LOCUS       DASPUL010000001         6181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1782360, whole genome shotgun sequence.
ACCESSION   DASPUL010000001 DASPUL010000000
VERSION     DASPUL010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1782360"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VEJ00_00005"
     CDS             <1..615
                     /locus_tag="VEJ00_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXW99564.1"
                     /translation="TFVRVNLALLIGAAWTIPAGVAIGFNPRMARIAQPLAQIAASVP
                     ATALFPVVLLLLIRVGGGLGIGSIVLLLLGTQWYILFNVIAGAIAIPTDLKECCSVFR
                     LRGLQRWRKLILPGIFPYLVTGLVTASGGAWNASIVAEYFHFKGQIYTTAGLGATISQ
                     ATDAGNFDLLLAATMMMAATVVTINRLVWRRLYGLAETRFRLET"
     gene            788..2536
                     /locus_tag="VEJ00_00010"
     CDS             788..2536
                     /locus_tag="VEJ00_00010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HXW99565.1"
                     /translation="MSRVVDFPGLAARLAPVGKVRDLYRRVQQSAEEFGFEALLAEMR
                     VELRVEAADQSRIPATGPVVAVANHPYGILDGAILAALLTRVRPDVKVLTNFLLADVP
                     ELERHCIFVDPFQTDRSIESNRQALKQSLAWLQQGGMLAIFPSGEVSSWQMPSAEIAD
                     PEWNDTAARLMRETGASALPVYFCGYNSVGFQLLGMIHPQLRTVFLLQEFLRQEGRTV
                     EVRVGSEISNEAISAIPSNQEATHYLRSRTYLLARRTKPDPWREFASRSKLALKPQQP
                     VAAPLPPELLEKEIASLPKDRCLAQNGDLAVYLGTAAEMPQVLREVGRLREVTFRIAG
                     EGTGKSLDLDRFDDYYWHMVLWHRTRRELVGGYRAGNTAEILAQHGIEGLYTSTLFRY
                     DERIFQKLGPALELGRSFVRAEYQRQYAPLLLLWKGIARLIVSRPQTPVLFGAVSISN
                     DYTEASREMIYRFFEARMQEDELAGMIEPRRPFRSARLRQWDCRSMCRALRDLDELSQ
                     PITDVEADGKGLPVLLRQYAKIGGKMLGFNVDHKFSNVLDGLVAVDLRQTDPFVLERY
                     MGRDGAAAFCRFHSLG"
     gene            complement(2562..3053)
                     /locus_tag="VEJ00_00015"
     CDS             complement(2562..3053)
                     /locus_tag="VEJ00_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXW99566.1"
                     /translation="MLRDKSLIPLSRQHQHALALCVRIDRAAPIPDADLETWQAEIAQ
                     HFQAEIGIHFAAEEKVLFPAARRFKGLSKMVSELLADHVVLRERFAQAENRSLSAAEM
                     SAFGKQLTAHIRKEERQLFEDLQKQMSPEELERLGQKLDDALRDAAQACALPETARLR
                     PTK"
     gene            3261..3449
                     /locus_tag="VEJ00_00020"
     CDS             3261..3449
                     /locus_tag="VEJ00_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99567.1"
                     /translation="MVATGELIRSMNYVDDITATLRRICVAIPSMTAEERQRLAEHLR
                     AAGASITAAIAELEKAGH"
     gene            3450..4319
                     /locus_tag="VEJ00_00025"
     CDS             3450..4319
                     /locus_tag="VEJ00_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXW99568.1"
                     /translation="MAAVNTIAVIGAGIMGRGIAHAAALGGYRTILEDLLPAALRKAE
                     SEIRANLDNAVELGKVTAPDADSAFRRLEYAGSVEEAAREADLVIEAVPEELESKVEI
                     FTLLDKICRPTTILASNTSSLSVTEIASVTYRAKKCLGMHFFSPVHKMKLLEVVRALE
                     TDEETLSTAVEVGRRMGKETIVIRESPGFITSRINAMIGNEAFYMLQEGIASAADIDK
                     ALKLGLNHPMGPFELVDLVGLDTRLSILEYLHKSLGEKYRPAPLLVQYVKAGRLGRKS
                     GRGVFDYPEAASK"
     gene            complement(4381..>6181)
                     /locus_tag="VEJ00_00030"
     CDS             complement(4381..>6181)
                     /locus_tag="VEJ00_00030"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HXW99569.1"
                     /translation="KNVSNYCNIGLCRDNFRETFQWGFPRENKPMTPLQPRLLYGFVL
                     SGLAALSLVVGSATAQTVTTPSGTFPSQTYQGVVAGGTTDFLGIRYAAAPTGALRFAA
                     PTPPTGVTGTINAVQFGSSCPQTPTPFGQGSIDEDCLFLNIFIPNPPVSSKNRFPVMV
                     FIHGGAFVTGEGSDYNATDLANQGKVVVVTINYRLGILGYMANAALDAESVSGASGNY
                     GLADQQFALQWVRQNIAAFGGNPDQVTIFGESAGGFSVCANVVSPVAAGLFQRAISES
                     GPCATNFPTKAAAETEGAGIAAALGCAQATSAATVACLRALPVSDILAVQNGITSQSS
                     LASLTAFFPNVDGVLIPEEPLQALATGEFNHLPLLMGTNHDEGRLFIALDFDLNPEAG
                     PLTAAEYPTAVAGIAAAAVAEGGSNNPALVAQISEEILQRYPLSAFPSPDLALSAIFT
                     DSAFSCPARLTRQLTSLQVATFGYEFNDENAPMIFLPPVSFPYGATHTDELQFLFPFG
                     GTLTANEQTLATTMKSYWTNFAAHGNPNQLGQPFWPEFGILFNDDQSLTAPTPAVEFN
                     FAKVHQCNFWTAVLLQTSLPDLAEQLRSSSIAH"
BASE COUNT         1209 a         1798 c         1903 g         1271 t
ORIGIN      
        1 acgtttgtgc gcgtcaatct cgccttgttg atcggcgccg cttggacaat tcccgccggg
       61 gtcgcgatcg gattcaaccc acgcatggca cgtatcgcgc agccgctggc gcagatcgcg
      121 gcctcagtgc cggcgacggc gttgtttccc gtcgtgctgc tgcttctaat tcgtgtgggc
      181 ggagggttgg gcatcggctc catcgtgctt ctgctgctcg gaacgcagtg gtacatcttg
      241 ttcaacgtga ttgcgggtgc gatcgccatt cccacagatt tgaaagagtg ctgctcggtc
      301 ttccggctta gagggctgca gcggtggagg aaactaatcc tgcccgggat ctttccctac
      361 cttgtgaccg gattggtcac ggcatcgggc ggcgcgtgga atgcgagcat cgtcgccgag
      421 tactttcact tcaagggaca gatatacacc acggctggcc tgggggcgac tatctcgcaa
      481 gcgaccgatg ccggcaattt cgacttgctg ctggcggcga ccatgatgat ggcggcaacc
      541 gtcgtcacca tcaaccgtct ggtgtggcgc aggctttatg gcttggctga aacgcgtttc
      601 cggcttgaga cgtagatctg gttttcagtc gggtcaaaat gcagtggtta ccacaggtta
      661 cttccgagct tactcagcac tacatttcat caatctttaa cctgagcttc atcctccagc
      721 aaactgattg cactatttct gtagcatgcc cttagaccag gcgcgcgcag agcagacgca
      781 agatgtattg tcgcgggtcg ttgattttcc cgggcttgcg gcccggctgg cgcccgtggg
      841 caaggtgcgc gatctctacc gccgcgtgca gcagtcagcg gaggaattcg gttttgaagc
      901 gttgcttgct gaaatgcggg ttgagctgcg cgtcgaggct gccgatcagt cgcgcattcc
      961 cgccaccggt ccggtcgtgg cggtggccaa tcatccctat ggcattctcg atggcgcgat
     1021 cctcgctgcg ctgcttacgc gtgtccgtcc ggacgtgaaa gtgctcacca acttcctgct
     1081 ggctgacgtg ccggaactgg agcgtcactg catttttgtg gaccccttcc agacggaccg
     1141 ctcgattgaa tccaaccgcc aggcgctgaa gcagtccctg gcctggctgc agcagggggg
     1201 catgctggcc atatttccat ccggcgaagt ctcttcgtgg cagatgccat cagcggagat
     1261 cgccgatccg gagtggaacg acacggcggc gcgtctcatg cgagaaacgg gagcttccgc
     1321 cctgccggtc tacttctgcg gatacaacag cgtgggattc cagcttctgg gcatgattca
     1381 tccgcagctg cggaccgtgt ttctgctgca ggagttcctt cgccaggaag gcagaaccgt
     1441 ggaagtccgc gtggggtcgg agatttcgaa cgaagcgatc tcggcaatcc ccagtaatca
     1501 ggaagccacc cactacttgc gctcgcggac ctacctgctg gcgcggcgga ccaagcctga
     1561 cccctggcgc gagttcgcta gtcgctccaa gctcgctttg aaaccccagc agccggtggc
     1621 ggcgccgctg ccgccggagt tgctcgagaa agaaattgcg agccttccca aggaccgttg
     1681 cttggcccag aacggcgatc tggcggtcta tctgggaacg gctgctgaaa tgccgcaggt
     1741 gctgcgcgag gtgggccgat tgcgggaagt gacctttcgc attgccggcg aaggaacggg
     1801 caagagcctc gaccttgacc gcttcgacga ctactactgg catatggtcc tatggcaccg
     1861 gaccaggcgc gagcttgtgg gagggtaccg cgccgggaat acggccgaga tcctcgcaca
     1921 gcatggaatc gagggcttgt acacgagcac gctcttccgc tacgacgaac gaatcttcca
     1981 gaagctggga ccggccctgg aactgggccg ctccttcgtg cgcgccgagt atcagcgcca
     2041 gtacgcgccg ctgctgctgc tgtggaaggg catcgcccgg ctgatcgtgt cacggccgca
     2101 gacgccggta ttgttcggcg cagtcagcat cagcaatgac tacaccgagg cgtcgcgcga
     2161 gatgatctac cgtttcttcg aagcgcgcat gcaagaagac gaactggcgg gcatgatcga
     2221 gccgcggcgt ccatttcgct ccgcgcggct gcgccagtgg gactgccgca gcatgtgccg
     2281 cgccctgcgc gatctcgatg agctctcgca gcccatcacc gacgtcgagg ctgacggcaa
     2341 gggccttcct gttctgctgc ggcaatatgc caagattggc ggcaagatgc tggggttcaa
     2401 cgtcgaccat aagttctcga acgtgctcga cggcctggtc gcggtggatt tgcggcaaac
     2461 cgatccattt gtcttggaac gatacatggg gcgggacggc gcggcggcat tttgccggtt
     2521 ccactcactc ggctaacacg cgtctctgaa cactccacag actacttggt cggtcgcaat
     2581 cttgcagttt caggcagagc acaggcttgt gcggcatctc ttaacgcgtc gtccaacttc
     2641 tggcccaggc gttctagctc ttccgggctc atctgctttt gcaagtcttc gaacaattga
     2701 cgttcctctt tgcggatgtg agcggtgagc tgttttccga aggccgacat ttctgctgcc
     2761 gacaggctgc ggttctccgc ctgcgcgaag cgttcccgca gaacgacatg gtcagccaga
     2821 agctctgaca ccatcttgga caatcccttg aatcttcttg ccgccggaaa taacaccttc
     2881 tcctccgcgg caaaatgaat tccaatttca gcctgaaagt gctgggcgat ttccgcttgc
     2941 caggtctcga gatcggcatc tgggattgga gcggcgcggt cgatacggac acaaagagct
     3001 agcgcatgct ggtgttgccg cgagagtgga atcaggctct tgtcccgtag catgaggcaa
     3061 aaaatccggc agaacgactg ccaacagatc aacctatgcg cgcgatcgat ttctggatta
     3121 tatccggccc aatccgctcc gttgtcttta ctaaatccgt gcccacgtcc aaaataggag
     3181 gttcggcttg ggcgcgagat aggccggagg actcgtcgaa cctacacgcc gccggctgag
     3241 caataaagac aggagccctc atggtagcga ctggtgaact gatccgttcg atgaattatg
     3301 tagacgacat taccgcgacc ctgcgccgga tctgcgtcgc aattcccagc atgactgcgg
     3361 aggagaggca acggctggca gaacatctgc gagccgccgg agcctcaatt acggcggcca
     3421 tcgcggagct cgaaaaggcg ggccactaag tggctgcggt caacacgatc gcggtgatcg
     3481 gggctggaat catggggcga ggcatcgccc acgccgccgc gctcggcgga tatcgcacta
     3541 ttctcgaaga cctcttgcct gcggctttgc gcaaggccga atcggaaatc cgcgctaatc
     3601 ttgataacgc cgtcgaactc ggcaaggtaa ccgcacccga cgccgattct gctttccgac
     3661 ggctggaata cgccggttcg gttgaagaag ctgcgcgtga ggccgatctg gtgatcgagg
     3721 cggtccccga ggagttggag tccaaagtag agattttcac cttgctcgac aaaatctgcc
     3781 ggcccacgac gattctggcg tcgaacacat cctcactcag tgtgactgag atcgcgagcg
     3841 tcacctaccg cgcgaaaaag tgcctcggga tgcacttttt cagtcccgtg cacaagatga
     3901 agctgctcga ggtggtgcgc gcgctcgaaa ccgacgaaga gaccctgagc acggccgtcg
     3961 aagtcggccg gcgcatgggc aaggaaacga tcgtcatcag agagtcgccc ggcttcatca
     4021 ccagccgcat caacgccatg atcggcaacg aggcgttcta catgctgcag gaaggcatcg
     4081 cgtcggcggc ggacatcgac aaggcactca aattggggct gaaccatcct atgggaccct
     4141 tcgagctggt cgatctggtc ggcctggata cccgcctgag cattctcgag tacctgcaca
     4201 agtcgctggg agagaagtac cgtcccgcgc cgttgctggt gcaatacgtg aaggccgggc
     4261 ggctggggcg gaagtcagga cgcggcgtgt tcgactatcc ggaagcggca tcaaagtgag
     4321 cgggtccggc ggcatctcgc agcgttgcaa gatgccgcca atcttgattc cagcccgcga
     4381 tcaatgagcg atgctgctgc tccggagctg ctcggcgagg tccggcaggc tggtttgcag
     4441 cagaactgcc gtccagaagt tgcattggtg gaccttcgcg aaattaaact caactgcagg
     4501 cgtcggtgcc gtcaatgact gatcgtcatt gaagagaatg ccgaactcgg gccaaaaggg
     4561 ttggcccagc tggttcggat tgccgtgggc cgcgaagttt gtccagtagc tcttcatcgt
     4621 agtcgccagc gtttgctcgt tcgcggtcaa tgtgccacca aatggaaaga gaaactgaag
     4681 ctcgtcagtg tgagttgctc cgtaaggaaa gctcacgggt ggcagaaata tcatcggtgc
     4741 attctcgtcg ttgaactcat acccgaaagt cgctacctgc aacgacgtta actggcgcgt
     4801 gagacgcgct gggcaactga atgcgctgtc cgtgaagatg gcggacaacg ccaggtcggg
     4861 actcggaaaa gcgctgagcg gatacctctg caggatctcc tcgctgattt gtgcgaccag
     4921 tgccggattg ttgctcccgc cttccgcgac cgcagcggca gcgattcctg cgaccgccgt
     4981 ggggtattcc gcggccgtga gcggtccagc ctccggattc aggtcgaaat ccagggcgat
     5041 gaagagccgg ccctcgtcgt ggttcgttcc catcagcagg ggcaggtggt tgaattcacc
     5101 tgtcgcaagg gcctgcaggg gttcttctgg aatcaatacg ccatccacat tcgggaagaa
     5161 cgccgtgagt gaggcaagac tgctctggga cgtgattccg ttctgcactg ccaggatgtc
     5221 cgacacgggc agcgcgcgca ggcaggctac agtcgcggcg ctggtggctt gcgcgcagcc
     5281 gagggcggcc gcgattccag ccccttcggt ttctgcggct gccttggtcg gaaagttcgt
     5341 agcgcaaggg ccgctctccg aaatcgcacg ctggaacaat cctgccgcaa cgggcgagac
     5401 aacgtttgcg cacacgctga agccgccggc ggattcgcca aagatggtta cctgatcagg
     5461 attgcccccg aatgctgcga tattctgacg tacccactgc agggcgaatt gttgatcggc
     5521 aaggccgtag ttgcccgagg cgccgctcac cgattcggca tccagcgcgg catttgccat
     5581 gtagccgaga attcccagcc gataattgat ggtcacgacg acgaccttgc cctggttcgc
     5641 cagatccgtg gcgttgtagt cgctgccttc ccctgtcacg aacgctccgc cgtggatgaa
     5701 gaccattacc gggaagcggt ttttcgatga aaccggggga tttggtatga agatgtttag
     5761 gaacaggcag tcctcgtcaa tactcccctg cccgaacggc gtgggagttt gcgggcaact
     5821 gctgccaaat tgcacggcgt tgatcgtccc tgtcaccccg gtcggagggg tgggggccgc
     5881 aaagcgaagc gcgcccgtcg gggccgcggc atagcggatc ccgaggaagt ctgtggtgcc
     5941 gccggcgact acaccttgat aggtttgaga cggaaaagtt cctgagggtg tggtgaccgt
     6001 ctgggcggtt gcactgccca ccacgaggga caacgctgcc aatccagaca aaacgaatcc
     6061 gtacaagagt cgaggctgaa ggggcgtcat cggtttgttc tcccgcggga atccccactg
     6121 aaaagtctcg cggaaattat cacggcaaag tccgatattg caatagttac taacattttt
     6181 t
//
LOCUS       DASPUL010000002         3306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1371109, whole genome shotgun sequence.
ACCESSION   DASPUL010000002 DASPUL010000000
VERSION     DASPUL010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1371109"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VEJ00_00035"
     CDS             <1..816
                     /locus_tag="VEJ00_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW99570.1"
                     /translation="NGAGKTTTIEILEGLLQPTSGEVSILGHTWNDNEREMREWLGIS
                     LQETRLSEKLSVSETIELFASFYREPRSSDEVLEQLQLTEKADSWVGKLSGGQRQRLA
                     VATALVCNPKILFLDEPTTGLDPQSRRQLWDIIRGFQRDGGTVLLTTHYMDEAERLCD
                     RLAIVDHGQIIAEGTPPDLIDRLGGHHVVEFAVSGGPDGAGLEAWRRLPSVESVREDE
                     DIVALNVKEPHLTIPALLDAVDRQGKQLEHLTTRQASLEDVFVRLTGRHLREE"
     gene            997..1494
                     /locus_tag="VEJ00_00040"
     CDS             997..1494
                     /locus_tag="VEJ00_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXW99571.1"
                     /translation="MRQIMSFATISLVLLVVFSGPARAHAPARASQEMNLYPANVDAR
                     AEIKEAEERAAKDHKRVLLVFGANWCGDCHVLDRAFHRPDFASAIAGYEVVHVDIGDD
                     GKKNNDLARQFEVPLEKGIPALAVLESDGKLVVSQKNGEFENARAMNPQAFLEFLNKW
                     KLPAR"
     gene            1671..2783
                     /locus_tag="VEJ00_00045"
     CDS             1671..2783
                     /locus_tag="VEJ00_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW99572.1"
                     /translation="MSTTQEIPVSSARPPRVTSHRNGRWSGYRHLLMARMLELKREPE
                     VVFWVFAFPLLLALGLGIAFRNKPADVVSVAIVAGTGSPDAAAMLARSPQHEMFKPQI
                     LGAAEAQTGFRLGKFDLVIEPDGKGGFAYRYDPARPESVLAKAEVNDALQGAAGRKDA
                     VASTAVASSEPGSRYIDFLIPGLLGMNLMNSGMWGIGFALVDMRQRKLLKRFVATPMR
                     RGDFLLALMSSRLVLMVIEIGLLLVFGVLFFHMRVLGSVFAIALIGGIGALTFGGVGL
                     LTASRAQKIESISGLINLVMMPMWIFSGVFFSYERFPAVIHPLIKALPLTALNDALRA
                     CILEGTPILHQWPRLLVMGLWGGISFVLALKWFRWT"
     gene            complement(2824..>3306)
                     /locus_tag="VEJ00_00050"
     CDS             complement(2824..>3306)
                     /locus_tag="VEJ00_00050"
                     /inference="COORDINATES: protein motif:HMM:NF022431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HXW99573.1"
                     /translation="GLLNDALAYLNQERDLRGYDAKLGWIHATAHTADLLRELAENSL
                     LTKADQTSILRAISERLSTARSVYTHGEQDRLALAVAAIIARADFDADALQAWIQQMQ
                     DTDNAMWSHTPIPLDEMARYQNDTYMLEALTARLGPAHAATESAARAREVVFAAVRER
                     "
BASE COUNT          603 a         1034 c         1010 g          659 t
ORIGIN      
        1 aacggagcag ggaagaccac gaccatcgaa attctcgaag gcttgctgca gccgacttcg
       61 ggagaagtct cgatcctcgg ccacacctgg aacgacaacg agcgcgaaat gcgcgagtgg
      121 ctgggcattt cgctgcagga aacgcgcctg tcggaaaagc taagcgtaag cgagaccatc
      181 gaactgttcg ccagtttcta tcgcgagccg cgctcgtcgg acgaagtgct tgagcagttg
      241 caactcaccg aaaaagctga ttcctgggtc ggcaagctct ccggtggaca gcgccagcga
      301 ctggcggtgg cgacagcgct tgtctgcaat ccgaagattc tgtttctcga cgagcctacg
      361 accggcctcg atccgcagag ccgccgccag ctctgggaca tcatccgcgg atttcagcgc
      421 gatggcggca ccgtgctgct cacgacccat tacatggacg aagccgagcg cctctgcgac
      481 cggttggcca tcgtcgatca cggccagatc atcgccgagg gcacgccccc cgatctgatc
      541 gatcgcctgg gcgggcatca cgtggtggag ttcgccgtaa gcggcggccc cgacggcgcc
      601 gggctcgagg cttggcgccg cctgccgagc gtcgagtcgg tacgcgagga cgaggacatc
      661 gtcgccctga atgtgaaaga accgcacctg accatccccg cgctcctcga cgccgtcgac
      721 cggcagggga aacaactgga acacctcacc acgcggcaag caagcctgga agacgtcttt
      781 gtacgcctga ccggccgcca cctgcgggag gagtgagaga atcgatggag ttttttgctt
      841 tctcagcgaa ctctgcggcc tttcttagcg atctttgcgg taaacgtttt tagatcgcaa
      901 agcgccacgg taggcccgga aatcacaacc ggcgggcagt ttcagttatc gtgcaacccc
      961 gggtcgcgga aacctgcaca aagaaggaag gtcgagatgc gacagatcat gagcttcgca
     1021 acgatttctt tagttttgct ggtggtcttt tctggtccgg cgcgggcgca tgctccagct
     1081 cgcgccagcc aggagatgaa tctctatccc gcgaacgttg acgcccgcgc cgagatcaaa
     1141 gaagccgagg agcgcgcggc gaaagaccac aagcgggtcc tgctcgtctt cggcgcgaac
     1201 tggtgcggag actgccacgt cctcgaccgc gcctttcatc gccccgactt cgcttctgcc
     1261 atcgcgggct acgaggtcgt tcatgtcgac atcggcgacg acggcaaaaa gaacaacgat
     1321 ctggcccggc agttcgaggt gccgctggag aaaggcatcc cggcgttggc agtgctggaa
     1381 agcgacggga aactcgtggt cagccagaag aacggcgagt ttgaaaacgc ccgcgccatg
     1441 aatccgcagg cattcctgga gtttctgaac aagtggaagc tgccggcgcg gtaattgttt
     1501 cggttgacag ccggcttcgt gtttgagctt ttcttcgcga tctttgcggc ctttcctctg
     1561 cgcactttgc ggtcaaagct ctttaccgcc aagaccgcaa agaaaccgcc gcaagggtcg
     1621 cgaagacgag cgaaaactca agtgcatgag ctgcaggatg gggatcttcg atgagcacta
     1681 cccaggaaat cccggtttcg tcggccaggc ctccgcgcgt gacttcgcac cgcaacggcc
     1741 gctggtccgg ctatcgccat ttgctgatgg cgcgcatgct cgaactgaag cgcgagccgg
     1801 aggtcgtgtt ctgggtgttt gctttcccgc tgctgctggc tctgggcctg ggcattgcct
     1861 tccgcaacaa gcccgccgat gtggtctcgg tcgcaatcgt tgccggaacc ggatcgccag
     1921 acgccgcggc catgctcgcc cgctcacccc agcacgaaat gtttaagccg cagatcctgg
     1981 gcgcagccga agcccagacc ggattccggc tgggcaagtt cgatctggtc atcgagcctg
     2041 acggcaaggg cggattcgcc tatcgttacg atcctgcgcg tcccgaaagc gtgctggcca
     2101 aagccgaggt caacgacgcg ctgcaaggcg cggctggccg caaagacgcg gttgcctcaa
     2161 ctgcggtcgc ctcctccgag cccggctcgc gctacatcga tttcctcatt cccggcctgc
     2221 tgggcatgaa cctgatgaac tccggcatgt ggggcatcgg cttcgcgctg gtcgacatgc
     2281 gccagcgcaa gctgctcaag cgcttcgtcg cgacgcctat gcgccgcggc gatttcctgc
     2341 tcgcgctcat gtcgagccgc ctcgtcctga tggtcatcga gatcggcctg ctgctggtct
     2401 tcggtgtgct gttctttcac atgcgcgtgc tgggatcggt attcgccatt gccctaatcg
     2461 gcggtattgg cgcgctcacc tttggcggcg tcggcctcct gaccgccagc cgcgcccaga
     2521 aaatcgagag catcagcggt ctgatcaacc tcgtgatgat gccgatgtgg attttttccg
     2581 gcgtcttttt ttcttacgag cgcttcccgg cggtgatcca tccgctgatt aaagccctgc
     2641 ccttgaccgc gctgaatgat gccctgcgcg cctgcattct tgagggcact cccatcctgc
     2701 accaatggcc gcgcctgctg gtgatggggc tgtggggtgg aatctcgttc gtgctcgcgc
     2761 tgaagtggtt ccgctggacg tagcgcggtg aggtttcaga gtccaatccg gccgagcatc
     2821 attttatcgc tcacgtacgg cggcaaaaac gacctcgcgc gcccgtgcag ccgactccgt
     2881 tgcggcatgc gccggtccta ggcgcgccgt cagcgcttcc agcatatagg tatcgttctg
     2941 atatcgcgcc atctcgtcga gcggaatcgg cgtgtggctc cacatggcgt tatccgtgtc
     3001 ctgcatttgc tggatccagg cctggagggc atccgcatca aagtcggccc gtgcgatgat
     3061 cgccgccaca gccaacgcca accggtcctg ttcgccgtgc gtgtagacgg accgggcggt
     3121 agacagccgt tcagaaatcg cgcgcaggat gcttgtctga tctgccttgg tcagcagcga
     3181 attctccgcc agctcccgca gcaggtccgc ggtgtgcgcc gtcgcgtgga tccatcccag
     3241 tttcgcgtca tagccgcgca gatcacgctc ctggttcaga taggccagtg catcgttgag
     3301 caggcc
//
LOCUS       DASPUL010000003         3414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_548653, whole genome shotgun sequence.
ACCESSION   DASPUL010000003 DASPUL010000000
VERSION     DASPUL010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548653"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..460
                     /gene="rpmH"
                     /locus_tag="VEJ00_00055"
     CDS             305..460
                     /gene="rpmH"
                     /locus_tag="VEJ00_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXW99574.1"
                     /translation="MPKRTFQPNRRKRSKKHGFRVRMRTQGGQKVLSRRRAKGRKRVS
                     VKPGFRE"
     gene            523..1014
                     /gene="rnpA"
                     /locus_tag="VEJ00_00060"
     CDS             523..1014
                     /gene="rnpA"
                     /locus_tag="VEJ00_00060"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXW99575.1"
                     /translation="MSAVAPESNKRELHQGVPTRDSGSGFPRSLRLLRHADFDRVYES
                     GRRHFSASLTVFYLPRPAEQAAAAKSAPPSGPRVGFTVGRALGGAVQRNRMKRRLREA
                     VRLSLPKTRLSVDIVINPKKSLLTTDFEAVLNEVRRAFVVIEQRLGDKAESKSTGPQI
                     NRR"
     gene            1165..1479
                     /gene="yidD"
                     /locus_tag="VEJ00_00065"
     CDS             1165..1479
                     /gene="yidD"
                     /locus_tag="VEJ00_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXW99576.1"
                     /translation="MKMLDIPKLVALQILRAYKWAISPLFPPACRYVPTCSEYAMEAV
                     ERYGVLRGGLMAIVRLLRCHPFARGGYDPVGQNSEGHEFRACGENPAARIVVEERPFR
                     AA"
     gene            1567..3300
                     /gene="yidC"
                     /locus_tag="VEJ00_00070"
     CDS             1567..3300
                     /gene="yidC"
                     /locus_tag="VEJ00_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXW99577.1"
                     /translation="MADYQNPQHEPGSERRLLLIFLVTFVVMLAFQPLLKKYFPQPPA
                     QQPTQTAQSETASTQPAPAPPATPPPGVAKQAASETETVIENDLYRITFTNRGAQVKS
                     WILKKFDNDAQNGPLDLVNPAAAPKYGYPLSLWTYDETLRNRLSSALYLASEEGKQTS
                     PTTLTFEYSDQDLAVQKTFRFDQTYVISVESSVAYKGGTIAALPAWPSGFGSQATPAF
                     YASGLINYQFNKNIERLAIKKISGGATIPGPFHWAGPTDQYFAAVFIPDDPASATMVT
                     LRNPLSVLRDPQKRDSKETVNVEVLGSAVGSLRGPTSERLFAGPKELEVLQKISVPGV
                     ADNDLNGLVDFGWWGIIAKPLFLWLKWTYHHMVPNWGWAIVVQTLIITIALLPLRITQ
                     MKSMLKMQRIQPQIKSIQDKYKKYSLRDPRKAAMNEEVSALYKKEGVNPVGGCLPMLI
                     QFPFLIAYYRMLGVALDLRHAHWLWIHDLSAADPFYVLPIFMIVSMLVTQKMTPQAGM
                     DPAQQRMLTVMMPLMMGFIFFRLAAGLNLYYAESNLISIAQQAIMNRTSLGREMREMM
                     AKRARKKDKDK"
BASE COUNT          716 a         1107 c          930 g          661 t
ORIGIN      
        1 ggatcacctt cggtcccaat ccctccggca gttgctcgat caacgtcacg gcaaccgtga
       61 actccctgag cagttgcacg atcaccggca cagtgtgcgg gcaatcggtc agcggaaact
      121 gctaggctac ggtttcgacc cgtgtcacac gaaggccact cagcagggag tgccttcgtg
      181 tgacttcgcg tttttgggga tgcccgtttt gaccggagct caccattggc gcttccccgg
      241 acggtgattt atactttgag attgccctaa taccgtcgag cgaatcagga gcagctaact
      301 tttcatgccc aagcgtacat tccaacccaa ccggcgcaag cggtccaaga agcacggatt
      361 ccgcgtgcgc atgcggaccc agggcggcca aaaagttctt tctcgccgcc gcgccaaggg
      421 gcgcaagcgg gtctcagtga aaccaggctt tcgcgagtag ccgtgtggcg cgggcacttt
      481 tgcccgcgtt cccgtgcacc gcataacctt ctgaacatca ttgtgagcgc cgttgcccct
      541 gaaagtaaca agcgagaact gcaccaaggt gtcccgacgc gggattccgg gtcgggtttt
      601 cctcgcagcc ttcggctgtt gcgccatgcc gattttgatc gcgtgtacga atccggtcgc
      661 cgccacttct cggcttcgtt aacggtgttc tatttgcccc gtccggcaga gcaagcggca
      721 gccgcgaagt ccgcgccgcc ttcgggacct cgcgtgggtt tcaccgtagg ccgcgccctc
      781 ggaggagcag tgcaacgcaa ccgcatgaag cgccgcttgc gcgaggcggt tcgcctttcg
      841 ttgcccaaaa cgaggttgag cgtcgacatt gtcatcaacc cgaagaagtc cttgctcacg
      901 accgacttcg aagcggtcct gaacgaagtt cgccgcgcct tcgtagtaat agaacagaga
      961 cttggagaca aggcggaaag caaatcgacc ggcccgcaaa tcaaccggcg atgaaaagca
     1021 atcgggactg gcgatgaaaa agactcggct ccggacggct cagcacaaca gtcccgcagg
     1081 acggaattca atagccggca cgtaagtgcc gggaaagagc gaggaaacta ggaccgagtc
     1141 ccctcgggga cggcacacct gcccatgaag atgcttgaca tcccgaaact cgtcgccttg
     1201 caaatcctgc gggcctataa gtgggcgatt tcgccgctgt ttccgccggc ttgccgctac
     1261 gttccgactt gttcggaata cgcgatggag gccgtggaac gctacggtgt tctgcgcggt
     1321 ggcttgatgg ccatcgtgcg cctgctgcgt tgtcatcctt ttgcgcgcgg cggctacgac
     1381 ccggtggggc agaacagcga agggcacgaa ttccgggctt gtggggagaa tcctgccgcc
     1441 cgaattgtcg tggaagagcg gcccttcagg gccgcgtaga gtggaacgga atttcgccgg
     1501 gcttttagcc cccgtggcca gtgagccatg cgcatgacct tcgagatttg aaacggaacc
     1561 tttaacttgg ccgactatca aaatcctcag cacgaacccg gcagcgagcg ccgcctgctc
     1621 ctgatcttcc tggtgacgtt cgtcgtcatg ctcgccttcc agccactgct gaagaaatat
     1681 ttcccccagc cgccggccca gcaaccgacc cagaccgcac agagcgagac tgcctccact
     1741 cagccggccc cggcgccacc cgccactccg ccacccggag tcgccaaaca agccgccagc
     1801 gaaaccgaaa ccgtcatcga gaacgatctc taccgcatca ccttcaccaa ccgcggcgcc
     1861 caggtcaaat cctggatcct gaaaaagttc gacaacgacg cgcaaaacgg cccgctcgat
     1921 ctcgtcaatc ccgcagccgc ccccaaatat ggctacccgc tctcgctctg gacttatgac
     1981 gagaccctgc gcaaccgcct gagttcggct ctctatctag cctcggaaga aggcaagcaa
     2041 acttcgccca cgactctaac cttcgaatat tccgaccagg atctcgccgt ccagaagact
     2101 ttccgtttcg accaaaccta cgtgatcagc gtcgaatcct ccgttgccta caaaggcggt
     2161 accatcgccg cgctgcccgc ctggccctcc ggcttcggca gtcaggccac tccggcgttc
     2221 tatgcctccg gcctcatcaa ctaccagttc aacaagaaca tcgagcgcct ggcgatcaag
     2281 aagatcagcg gcggtgctac catccccggc ccattccact gggccggccc taccgaccag
     2341 tacttcgccg ccgtcttcat acccgacgat cccgcctcgg ctacgatggt caccttgcgc
     2401 aacccgctct cggtcctccg cgatccgcag aagcgcgact cgaaagagac cgtcaatgtc
     2461 gaggtcctcg gctccgctgt cggcagcctg cgcgggccca catctgaacg cctgttcgcc
     2521 ggcccgaaag aactcgaggt tctccagaaa atttccgtcc ccggcgtcgc cgacaacgac
     2581 ctcaacggtc tggtcgattt cggctggtgg ggcatcatcg ccaagccgct cttcctctgg
     2641 ctcaagtgga cttaccacca catggtcccc aactggggat gggccatcgt ggtgcagact
     2701 ctgatcatca ccatcgcgct gctgcctctg cgcatcacgc agatgaaatc catgctcaag
     2761 atgcagcgca tccagccgca gatcaagtcc atccaggaca agtacaaaaa atacagcctg
     2821 cgcgatcccc gcaaggcggc catgaacgag gaagtcagcg ccctctacaa gaaggaaggc
     2881 gtcaatcctg tcggcggctg cctgccgatg ctcatccagt tcccgtttct catcgcttat
     2941 tatcggatgc tcggtgtggc tctcgacctg cgccacgccc actggctctg gattcacgat
     3001 ctgtcagcgg ccgatccctt ctatgtgttg ccgatattta tgatcgtcag catgctcgtc
     3061 acgcagaaga tgactccgca ggccgggatg gaccccgcgc agcaaagaat gttgaccgta
     3121 atgatgcccc tgatgatggg tttcatcttc ttccgcctgg ccgcaggctt gaacctgtac
     3181 tacgcggaaa gcaatttgat ctcgattgcg caacaggcca tcatgaatcg caccagcctc
     3241 ggccgcgaga tgcgcgagat gatggccaag cgcgcgagga agaaagataa agataagtaa
     3301 gctcctagct gctagcttct agctactagc ttggatcagc agatgctttg ggcaactttg
     3361 gggtcttggt cgcaactgca agagcgtgct gacagaaggc ccggctagaa gcta
//
LOCUS       DASPUL010000004         3556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1645564, whole genome shotgun sequence.
ACCESSION   DASPUL010000004 DASPUL010000000
VERSION     DASPUL010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645564"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VEJ00_00075"
     CDS             <1..1055
                     /locus_tag="VEJ00_00075"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXW99578.1"
                     /translation="LRVTKSDGSAESLEFDRIVLATGSRPAIIPALKLDSPRLMDSTG
                     ALNLEDIPASLLVVGGGYIGLELGSVYAALGTRVTVVEMLPGLLPGADRDLVAPLHKR
                     LEKMFEAILLNTTVAAVKDEGTGIRATLTGKDGNTQEKVFDRVLVSVGRKPNSEIPGL
                     EKTRVQIGARGFIQVNKQLQTDDPDIYAIGDVVGEPMLAHKAMHEGRTAVEAIAGHKV
                     AFEPNAIPAVVFTDPEVAWCGLTETQAQNENREVKVAKFPWAASGRAITLDRPEGLTK
                     LLIDPQTERVLGVGIVGVGAGEMIAEGVLAVEMAALAKDVALTIHPHPTLSETIMESA
                     EVFFGTSTDIYRPKRG"
     gene            1241..3037
                     /locus_tag="VEJ00_00080"
     CDS             1241..3037
                     /locus_tag="VEJ00_00080"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXW99579.1"
                     /translation="MISLPGRSSFLKAGFIVLLCLSAGLSPAQSTLQQLLQPASSTPS
                     SPASPTDPLGRTTPQSCVLGFLKAAQSGDYSIASQYLQMTAARRQAEGEQLAEKLNYV
                     LNHAFTGSAKGWSTQPEGTPQEGVPLGRQKLGTMSSGDVEADLEVVRMTDPSAGKIWL
                     ISSDTLTKVPELYDQAEARQVEHKLPGLLVKHQLAGMPLWQWLALLLAAPVAAAIGWL
                     VIAVLEIPVRWWARRRGHIDVANWREVSGPAWLLAGTLAHQSFARYLGMPLLPRHYYF
                     QLTSVAMIVGATWIFWRVVRWSLRRVRNRALAYGHVGTGSLMLLGERLVKAIIFVTGV
                     LAVLGSLGFNMTTALAGLGIGGLAIGFGAQQTIANLFGGVSVLGDEVIRVGDVCTFGD
                     RTGTVEDIGLRSTRIRTAERTLVAIPNGTVASINVENLSRRDKILFKTNLGLRHESKA
                     DHVRYVVSEIRRLLYSHPKVETKTARARLTDISTGSGSVEVFAYILTQDFNEFAAIRE
                     DLLLRMLDLVEESGTGFALPSQTLYIGRDAGVQKGKAETAVRKIDELRDGKQLPFPDY
                     HGEQISSFKGTIEYPPKESAVREPEEGKSKTG"
     gene            complement(3146..>3556)
                     /locus_tag="VEJ00_00085"
     CDS             complement(3146..>3556)
                     /locus_tag="VEJ00_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99580.1"
                     /translation="FQSLYIVDLVGALLSGHHIIGGTEYMFDPGLPLLIRLLSLFHVA
                     TPPLLLWAIRLLGYDRRGWKLQTLAAWIVLPLNYFWHPEMDVNWARGPFFHEQHVLPG
                     LVYLLGYLIVVPLTVYFPTHLFLNWLSRWSKPRA"
BASE COUNT          706 a         1034 c         1157 g          659 t
ORIGIN      
        1 cgctgcgggt cacgaagtcg gacggatcgg ccgaaagtct cgagttcgat cgcatcgtcc
       61 tcgcgaccgg ctcgcggcct gcgatcatac cggcgttgaa gctcgattcg ccgcgcctga
      121 tggattcgac cggggcgctg aatctcgaag atattcctgc gagcctgctg gtggtcggtg
      181 gcggatacat cggactcgaa ctcggctcgg tctatgctgc gctgggcacg cgcgtgaccg
      241 tcgtcgagat gctccccggg ttgcttccag gagcggatcg cgatctggtg gcgccgctgc
      301 acaagcggct ggagaaaatg ttcgaagcaa ttctgctcaa caccaccgtc gcggcggtca
      361 aggacgaagg tacgggaatt cgcgccacgc tgaccgggaa agatggcaat actcaagaaa
      421 aagtcttcga ccgcgtgctg gtgtcggtag gacgcaaacc gaattcggaa atccccggcc
      481 tggaaaaaac gcgcgtgcag atcggcgcgc gcggattcat tcaggtgaac aagcagttgc
      541 agaccgacga tccagacatc tacgcgatcg gcgatgtcgt tggcgagccc atgctggcgc
      601 acaaggccat gcacgagggc cgcaccgccg tcgaggccat cgccggacac aaagtcgcgt
      661 ttgaacccaa cgctattcct gcggttgtgt tcaccgatcc ggaagtggcg tggtgcggcc
      721 tgacggagac acaggcgcag aatgagaacc gcgaagtgaa ggtcgcgaaa tttccctggg
      781 ccgcttcggg gcgcgcgatc acgctcgatc gtcctgaggg tctgaccaag ctgctgatcg
      841 atccgcagac tgaacgcgtc ctgggcgtgg gcatcgtggg cgttggcgcg ggcgagatga
      901 tcgccgaagg cgtcctggct gtcgaaatgg cggcgctggc caaggatgta gcgctgacga
      961 ttcacccgca tcccacgctg tcggagacga tcatggagtc ggcggaagtc ttcttcggga
     1021 ccagcacgga tatctatcgg cccaagcggg gatgattccc gcagcagcta atgccggttc
     1081 tggctgtcga ctgcggtggc acacctgaag gcatgcgctg atacgaatct tttcttcgcc
     1141 cgagccagca atatccggcc gcagggtttc gttacaataa ttctcactcg cgattctgca
     1201 cgttctgcgt cgcccgcaca gagccgggcg ggagaatgcc atgatatctc ttcctggaag
     1261 gtcttctttt ctgaaagcgg gattcatcgt tctgctttgc ctgtccgcgg gtctgtcgcc
     1321 agcccagtcc acactgcaac agttgttgca gccggcttcg tccacaccaa gctcacctgc
     1381 ttcgcccacc gatccgctgg ggcgcacgac gccacagagc tgtgtgctgg gatttctgaa
     1441 agcggcgcag tcgggtgact acagcatcgc gtcgcaatat ctgcaaatga cggcggcccg
     1501 tcgccaggcc gagggcgagc agctcgccga gaagctgaac tatgtcttga accacgcctt
     1561 caccggtagt gccaagggct ggagcacgca gcccgagggg accccgcagg aaggtgtgcc
     1621 tctggggcgg cagaaactgg gaacgatgtc gtcgggcgat gtggaggcgg atctggaagt
     1681 ggtacgcatg accgatccga gcgcgggcaa gatctggctg atctcgtccg acaccctgac
     1741 gaaagttccc gaactctacg atcaggcgga agcgcggcag gtggaacaca agctgccggg
     1801 cttgctggtg aagcatcagc tggccggaat gcctttgtgg cagtggctgg cgctgctgct
     1861 ggcggcgccg gtggcagccg ccatcggatg gctggtgatc gctgtcttgg aaattcccgt
     1921 ccgctggtgg gcgcggcggc gcggccacat cgatgtcgcc aactggcgcg aggtttccgg
     1981 gccggcgtgg ctgctggcag gcacgctcgc ccatcaatcg ttcgcccgct acctgggcat
     2041 gccgctgctc ccgcggcatt actactttca gctcacgtcg gtggcgatga tcgtcggcgc
     2101 cacctggatc ttctggcgag tggtgcgctg gtcgctgcgc cgcgtgcgca atcgcgcgct
     2161 ggcctacggg cacgtgggca cgggctcgct catgctgctg ggcgagcggc tggtgaaggc
     2221 gatcattttc gtgacaggtg ttctcgcggt gctgggcagc ctgggcttca acatgacgac
     2281 tgcgctggcc ggtctgggca tcggagggct ggccatcggc ttcggcgcgc agcagacgat
     2341 cgccaatctc tttggcgggg tttcagtgtt gggggacgag gtcattcgcg tgggcgatgt
     2401 ctgcacgttt ggcgatcgca caggtacagt cgaagacatc ggcctgcgct cgacgcgcat
     2461 ccgcaccgca gagcggacgc tggttgcgat tcccaatggc accgtggctt ccatcaacgt
     2521 ggagaatctg agccgccggg acaagattct cttcaagacg aatctcgggt tgcgccacga
     2581 gtccaaagcc gatcacgtgc ggtacgtggt gtcggaaatt cggcgattgc tgtacagcca
     2641 tcctaaagtg gagaccaaga cggcacgggc acggctgacc gatatttcta ccggctcggg
     2701 tagtgtggaa gtcttcgctt atatcctgac gcaggacttc aacgaatttg cggcgattcg
     2761 cgaagatttg ctgctgcgga tgctggatct ggtggaggaa tcaggcaccg gctttgcgct
     2821 gccctcacag actctataca tcgggcgcga cgcaggcgtg cagaaaggaa aggcggaaac
     2881 cgccgtcagg aaaatcgacg aattgcgcga cgggaagcaa ttgccattcc cggattatca
     2941 cggcgaacag atatcgtcgt tcaaaggcac gattgagtat ccgccgaaag agtcagcagt
     3001 gcgcgagcca gaggagggaa agagcaagac aggatagatc ctatcgagaa tgctgccacc
     3061 gcggctaaag cccacactca cccgactttg tacgcggccc tgaagggccg ctctcccacc
     3121 atccagcgcg gggttcagca ccctcctaag cgcgcggctt cgaccaccgg ctcagccaat
     3181 tgagaaaaag atgggtgggg aaatacaccg ttagcggcac cacaatcaga tatccgagca
     3241 gatataccag gcccggcaac acgtgctgct cgtggaagaa cggtccgcgg gcccagttca
     3301 catccatctc cggatgccag aaatagttga ggggcagcac gatccacgcg gccagggtct
     3361 ggagcttcca tccccggcgg tcgtagccaa ggagccggat cgcccacagc agcaacggtg
     3421 gtgttgccac gtgaaacagg ctcaaaagcc ggatcaggag cggcaatccg gggtcgaaca
     3481 tgtattccgt gccgccgatg atgtggtggc cgctgagcaa agcgccaacg agatcgacga
     3541 tgtagagcga ctgaaa
//
LOCUS       DASPUL010000005         5883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1508981, whole genome shotgun sequence.
ACCESSION   DASPUL010000005 DASPUL010000000
VERSION     DASPUL010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1508981"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VEJ00_00090"
     CDS             complement(<1..596)
                     /locus_tag="VEJ00_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99581.1"
                     /translation="MPKRLSSAVSLALALAVSASTWFYMNRILRAQQVADATLHNRPR
                     GNLSDLYPRWLGARELLLHGRNPYSAEITREIQQGYYGRVLDPARPDDPKDQQGFTYP
                     AYVVFPLAPTVGFPFHAVQTGFRWLLIALAPLSVLLWVRVLRWKASCATIAILAILMV
                     GWLPMVQGIKLQQLSLLVSGLLAACGACLTGGWLFCAGA"
     gene            694..1605
                     /locus_tag="VEJ00_00095"
     CDS             694..1605
                     /locus_tag="VEJ00_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99582.1"
                     /translation="MRSRIAVSAAIGVASGAFCWFLMKHFHQDAADFRWAMHLAQRLL
                     AKQNPYDTPLEQYPITAAILALPFVRLQPELAAGLFWGVSSFLLAFGLTRHGYTRLLI
                     FFAYPYWAGILTAQWPPIIAASAFFPLLLPITMAKPQVGLPVFLTRLSRNGMLACAVL
                     LAASLAWMPHWPWLWLRQARNYEHFIPLLVLPGPLLVLALLRHRDRDAWLLFLAALMP
                     QRWFFDSFILWLIPCSRRQIVWTVFFSWGAGILRWYHTPHSFTEVGRWAVIFLYLPML
                     AVVLLRSPMNEARDNDRTTGDICPAPV"
     gene            1653..2780
                     /locus_tag="VEJ00_00100"
     CDS             1653..2780
                     /locus_tag="VEJ00_00100"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXW99583.1"
                     /translation="MRRRYWHYWLSSLLLVGLIPVLRSQNLPLKFDWITLGVAFWLIL
                     AAQSIFVAVLLALIGLHGAETWQPFITRCRENPVHAVVILVFFAVLVWTTTILRGFVL
                     TVDAVALLEMVQQRQRQDLKRTLSAILIPAAYLFFGFLLVLAYNCAIVSARFNFAYDP
                     ALSKIDRWILFGHSVSDLTHWAVQAFPLFFFRALEFIYFGMFPQIGAAIILVALGDGK
                     MRALQLIGTILLSYYLALAIFYIAPAQGPYYLCPAHFSRFPSSLQSWNVQHTLIPHAL
                     ALWRHQPIRRISTDYFIAFPCMHIVQPIIVLWFLRRWKRMVYALAAYDVLLVVAILLL
                     EMHYIVDFIAGIVVALLAIAITDGPLRRRGSGILSGAESAG"
     gene            2777..4060
                     /locus_tag="VEJ00_00105"
     CDS             2777..4060
                     /locus_tag="VEJ00_00105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HXW99584.1"
                     /translation="MTRSAAKELIWLVLATLAAASMAHFTFAIWSSSQPVDFSDLYAP
                     WWGSHELLLHSRNPYSPEIAHEIQAVIYGARLHPTPEDPSGIGGGFAYPPWAALLLWP
                     SLYLPFPVAQQLCFGLSVAATLLSLGLWMRALRLRVSTFEALTFALFVLGSFPTLQGL
                     ELHNLSLLAAALIAAALFLIYSDHLILAGIFLAASTFKPQFVVALIPWLALWTLAAWR
                     QRRPLAWSFLISMTLLIGTSEWLLPGWLLSFLHVIRAYRHYTYGHTLFDVWLTPRWGI
                     VASAVALAAAWAFCWSSRSEPADSPRFLLTASLLLAVTVVVIPTLAPHAQLLLLPGVL
                     CLWRERAAPGSPGLLARCLLFAVWALLAWPWVAALGLLLARLWLPAAALHQFWPIPVY
                     TSPVLPLAVLAALSGLARHTLGRVQPLAEHSSRTP"
     gene            4322..4483
                     /locus_tag="VEJ00_00110"
     CDS             4322..4483
                     /locus_tag="VEJ00_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99585.1"
                     /translation="MELLRTLWREQEGQDIAEYAVMLAVILVIVVGTIRLIGSNANNV
                     FSSVASSIQ"
     gene            4549..5409
                     /gene="cpaB"
                     /locus_tag="VEJ00_00115"
     CDS             4549..5409
                     /gene="cpaB"
                     /locus_tag="VEJ00_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HXW99586.1"
                     /translation="MNRTRLLMIGVVALGIGFVVSVYVFKNLQSKSGPVEPGVEVIVA
                     ADDLQVGAKVSEHDIKIIRVPASDLPAGAPRKKSDVLDHGVILPITKGDFILSTKLAG
                     DNAGAGLPALIPPGMRAVAVRVNDVVSVSGFVTPGSRVDVLLTGTPGGSGEEQTTTVL
                     QNIEVVASGSRLERTSTGEAQNTAVITLLVSPDDAQRLTLASSQGHIQLALRNPLDTR
                     AQDVMPASARGLYKGAPAPPAQPVVHRTVIKKPEAAPPPASTGVSVEIYQGDKKNEVN
                     CTEEKGCASK"
     gene            5480..>5883
                     /locus_tag="VEJ00_00120"
     CDS             5480..>5883
                     /locus_tag="VEJ00_00120"
                     /inference="COORDINATES: protein motif:HMM:NF025014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HXW99587.1"
                     /translation="MMVRSLSSVALLFVAALGLPLPAETAQQTDPGAAAQSSTPTETQ
                     TTTLPPQSQPVQGSAPLRVMVGKSLLINTTEQLKRISITDPAIATAQVVTPTQILVHG
                     KTPGEVSLLIWDELERSRSFDLRVDVDVSACA"
BASE COUNT          949 a         2018 c         1659 g         1257 t
ORIGIN      
        1 gcgcccgcgc agaaaagcca cccgcctgtg aggcacgccc cgcacgccgc cagcagcccg
       61 gaaaccagca ggcttagttg ctgcagcttg atgccctgca ccatcggcag ccagccgacc
      121 atcaggatcg caagaatcgc gatcgtcgcg cacgacgcct tccagcgaag cactcgcacc
      181 caaagcaaaa cgcttagcgg cgccagcgca atcagcagcc agcgaaatcc ggtttgcact
      241 gcgtgaaatg gaaaaccgac cgtcggcgcc agcggaaaca caacgtaagc gggatacgtg
      301 aacccttgct gatccttcgg atcgtccggc cgcgccggat cgagcacacg accgtagtat
      361 ccctgctgaa tctcccgcgt gatctctgcg ctgtagggat tgcggccatg cagtagcagt
      421 tcccgcgctc ccagccaccg agggtaaaga tccgaaagat tgccgcgcgg ccggttgtgc
      481 agggttgcgt cggcgacctg ctgcgcccgc agaatgcggt tcatgtaaaa ccacgtactg
      541 gccgaaactg caagggctag agcgaggctg actgcggatg acaagcgctt tggcaagatg
      601 gagatggctt cttcctttac gagtgaacgt cacgagcaat cccgcccgcg gcaaaccgcc
      661 tcatgttaca actgtgaggg aaaaaatccc ttcatgcgat cgcgcatcgc agtctcggcc
      721 gccatcggcg tggcgagcgg cgcattctgc tggttcctga tgaagcactt ccatcaggac
      781 gccgccgatt tccgctgggc aatgcatctg gcgcagcgct tgctcgcgaa gcaaaaccct
      841 tacgatacgc cgctcgagca ataccccatc accgccgcaa tcttggccct tccctttgtg
      901 cgcctgcagc cggaactcgc cgccggactg ttctggggcg tcagttcgtt tcttctcgcg
      961 tttggcctca cgcgccacgg ttacacccgg ctgctgatct tcttcgccta cccgtattgg
     1021 gcgggaattc taacggcgca gtggccgccg atcatcgccg ccagcgcgtt cttccctttg
     1081 ttgctcccga taaccatggc caagccccaa gtgggattgc ccgtcttcct cacgcgcctc
     1141 agccgcaacg ggatgctggc gtgcgctgtg ttgctggcgg caagccttgc atggatgccg
     1201 cactggccct ggctgtggtt gcgccaggcc aggaactacg agcacttcat cccgctgctc
     1261 gttcttccgg gacctttgct cgtgctcgcg ctgctccgtc accgcgaccg cgacgcctgg
     1321 ctgctgtttc tcgccgcgct catgccgcag cgctggttct tcgacagctt catcctctgg
     1381 ctcattccct gctcgcgccg ccagatcgtc tggactgtct ttttcagctg gggcgccgga
     1441 atcctgcgct ggtatcacac gccgcacagc ttcaccgaag tcggccgctg ggcggtgatc
     1501 ttcctctatt tgcccatgct ggcggtggtt ttgctgcgca gcccgatgaa cgaagctcgg
     1561 gacaacgatc gcacgaccgg cgacatttgt cccgcgcccg tttgattgtt taaactccga
     1621 ccttcctcgt gcatccccaa ctccgcagcc cgatgcgacg ccggtactgg cactactggc
     1681 tcagttcgct tctgctcgtc gggctcatcc ccgtgctgcg cagccagaat cttccgctca
     1741 aattcgactg gatcacactg ggcgtcgcct tctggctcat cctcgcggcg cagtccatct
     1801 tcgtggccgt gctgctcgct ctcatcggac tgcatggcgc tgagacctgg cagccgttca
     1861 tcacgcgttg ccgcgagaat ccggtgcacg ccgtcgtcat ccttgtcttc ttcgcggttc
     1921 tggtttggac gacgacgata ctgcgagggt tcgttctcac cgtcgacgcc gtcgccctgc
     1981 tcgaaatggt ccaacagcgc caacggcagg atcttaaacg gacgctctcc gcaatcctta
     2041 tcccggcggc ttacctcttc ttcggattcc tgctggtgct cgcctacaac tgcgccatcg
     2101 tctcggcgcg tttcaatttt gcctacgatc cggcgctctc caagatcgac cgctggatcc
     2161 tgttcggcca ctccgtctct gaccttaccc actgggccgt tcaggccttc ccgctctttt
     2221 tcttccgggc actcgaattc atctatttcg gaatgttccc ccagatcgga gcagccatca
     2281 tcctggtggc gctcggcgac gggaaaatgc gcgccttgca actcatcggg acgattcttc
     2341 tgtcttacta tctcgccctg gccatcttct atatcgcgcc cgcgcaggga ccgtactacc
     2401 tctgccccgc gcacttttcc cgtttcccat ccagcctgca gtcctggaac gtgcagcaca
     2461 cgctgatccc gcacgcgctc gcgctctggc gccaccagcc catccggcgc atctcgacgg
     2521 actatttcat cgcctttccc tgcatgcaca tcgtgcagcc gatcatcgtg ctctggtttt
     2581 tgcgccgctg gaagcgcatg gtgtacgcgc tggccgcgta tgatgtgctt ctcgttgtgg
     2641 cgattctgct gctcgaaatg cactacatcg tcgatttcat tgccggcatt gtggtcgccc
     2701 tgctggccat cgccatcacc gacggtccgc ttcgccgccg gggcagcggc attctctcgg
     2761 gcgcggagtc ggcaggatga cgcgctccgc ggccaaggaa ctcatctggc tcgtcctcgc
     2821 gactctggcc gcggcgagca tggcccactt caccttcgcc atctggagct ccagccagcc
     2881 agtcgatttt tccgatctct acgcaccctg gtggggatcg cacgagctcc tgcttcacag
     2941 ccgcaatccc tactcgccag aaatcgccca cgaaattcag gcggtgatct acggcgcgcg
     3001 acttcacccg acgcccgaag acccttccgg catcggcggc ggattcgcct atccgccctg
     3061 ggccgcgctc ctgctctggc cgtcgctcta cctgccgttt cccgtcgcgc agcagttgtg
     3121 tttcggcctc tcggtcgcgg caacgctgtt gagcctcggg ctttggatgc gcgcgctgcg
     3181 gcttcgcgtc tcgacgttcg aggcgctgac cttcgcactg ttcgtcctcg gaagcttccc
     3241 cacgctgcag ggtctggaac tgcacaatct cagcctgctc gccgcggcgc tcatcgccgc
     3301 cgctctattc ctgatctatt ccgaccacct catcctcgcc ggaatctttc tggcggcgtc
     3361 gaccttcaag ccacaattcg tggttgctct gatcccctgg ctcgccctct ggactcttgc
     3421 tgcctggcgc cagcgccgcc cgctggcctg gagtttcctc atctccatga cgctgctgat
     3481 cggcacgagc gaatggctgc tgccgggatg gctcctcagc ttcctgcacg tcattcgtgc
     3541 ctatcgccac tacacctacg gccatacgtt gttcgatgtc tggctgaccc cacgctgggg
     3601 aatcgtagcc tccgccgtgg cgctcgcggc ggcatgggcc ttttgctggt ctagtcggtc
     3661 ggaaccagcc gactcgccgc gcttcctgct gaccgccagc ctgctgctgg ctgtgacggt
     3721 cgtcgtgatt ccaacccttg ctccccacgc ccaattgctt ttgctcccgg gagttctgtg
     3781 cctgtggcgc gaacgcgccg cgcccggctc gcccggcttg ctggctcggt gcctgctgtt
     3841 tgccgtctgg gctctgctgg cctggccctg ggtcgccgcg cttggactgc ttttggcgcg
     3901 cctctggctt ccggcggccg cattgcacca gttctggcca attcctgtct acaccagccc
     3961 cgtcctgccg ctagcggtcc tggcggccct ctccggactg gctcgccaca ctcttggacg
     4021 tgtccaaccg ctagcagagc actcaagccg cactccatag ccttcctctc gggcgggccc
     4081 gtagccaccc cagctcaggc ctcaccgggt cgctctgaca cggcggtaat ctaattttct
     4141 ccccgtatgc aagaaattaa gtcctttctt tggtagtgca aggtatgatt acgctaggcg
     4201 aggaggcggt gcctcgtcct cttcgaccgt tgagtcgcga cgctgccgcg ctctcgcgtt
     4261 cgtgccagat cgagccggct actctactcg acgtaagcaa gtggtacaaa agggggtctc
     4321 tatggagctc ttacgcaccc tgtggcggga gcaggaaggc caggatattg ccgagtacgc
     4381 ggtgatgctc gccgtgattc tcgttattgt ggttggaacc atccgcttga tcggttccaa
     4441 cgccaacaat gtcttctcct ccgtcgccag ttcgattcag tagtgatgca gggccggcag
     4501 gaccgcaacg tcggagccgg ctgaggtttt cgcttttcgg ggagtgccat gaaccgcacg
     4561 cgtttgttga tgatcggagt cgtcgcgtta ggcattggct tcgttgtaag tgtgtacgtc
     4621 ttcaagaatt tgcaatccaa gtccggcccg gtcgagcccg gcgtggaagt cattgtcgcg
     4681 gctgacgacc tgcaggtcgg cgccaaagtg agcgagcacg acatcaagat tattcgcgtt
     4741 cccgccagcg accttcccgc gggcgccccg cgcaagaaaa gcgacgtgct cgatcacggc
     4801 gtgatccttc ccattaccaa gggcgacttc attctgtcca ccaagctggc cggcgacaat
     4861 gccggagccg gactgcccgc cctgattcct cccggcatgc gcgcagttgc ggttcgtgtc
     4921 aacgatgtcg tttccgtttc cgggttcgtg accccgggaa gccgcgtcga tgtgctgctc
     4981 accgggacgc cgggtggaag tggcgaagaa cagacgacga ccgtactgca aaacatagag
     5041 gttgttgcat ccggctcacg gctggaacgc acttccactg gcgaagccca gaacaccgcg
     5101 gtcatcacgc ttctggtttc ccccgacgat gcgcagcggc tgacgctggc cagttcgcag
     5161 ggccatattc aactggcatt gcgcaatcct ctcgacaccc gggcgcagga tgtaatgccc
     5221 gcgagcgcgc gcggcctgta caagggtgct cccgctccgc cggctcaacc tgtcgttcat
     5281 cgcaccgtca tcaagaagcc ggaagccgcc ccgcccccgg cttctaccgg cgtgagcgtt
     5341 gagatctacc agggagataa gaagaacgag gtcaattgca ccgaggagaa gggttgcgcg
     5401 tccaagtgat cgagagactt tgcagcttgc gagtggggtc cccagccgag aaatccccta
     5461 tcgggagaga aatcgaaaaa tgatggtccg ctcgttgtcg tccgtggccc tgctgttcgt
     5521 cgcagccctc ggccttcccc tgcccgcgga gaccgctcag cagacggacc ccggagcggc
     5581 ggcgcagtcc tccaccccga ccgaaaccca gaccaccacc cttccgccac aatcgcagcc
     5641 ggtgcagggg tcggcgccgt tgcgcgtgat ggtaggcaag tcgctgctca tcaacaccac
     5701 cgagcagctg aagcgcatct ccatcactga cccggccatc gccacggccc aggtcgtcac
     5761 gcccacgcag attctggtcc acggcaagac gccgggcgaa gtttcgctct tgatctggga
     5821 cgaactggaa cgctcgcgca gcttcgacct gcgcgtcgat gtcgatgtca gcgcctgcgc
     5881 cga
//
LOCUS       DASPUL010000006         5350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_686461, whole genome shotgun sequence.
ACCESSION   DASPUL010000006 DASPUL010000000
VERSION     DASPUL010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686461"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VEJ00_00125"
     CDS             complement(<1..393)
                     /locus_tag="VEJ00_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99588.1"
                     /translation="MKPHRGLIGTFAAFAGLIVAALAPGLGGSAAKASQPNVDAAKGA
                     SADGQKIRVAFVLTEGATMIDFAGPWEVFQDVGGVQGPNPFELFTVSESRSPIHTSGG
                     MTVTPDYTFADAPVPKIVVIGAQRGAPKL"
     gene            complement(663..1439)
                     /locus_tag="VEJ00_00130"
     CDS             complement(663..1439)
                     /locus_tag="VEJ00_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXW99589.1"
                     /translation="MIVPTIRTIIADDEGLARTKLRVLLNSESGVNVVAECRDGKQTV
                     AAVQSHKPDLLLLDVQMPDVDGFEVLKQIPAHEMPLVIFTTAFDHYAIRAFEAHALDY
                     LLKPFNQERLHHALERVKTELLKSHEQTLRTRILDLLGEVKPDSPQLRRLVIRTAGRV
                     VFLDLNEVDWIEAAANYVKLHVGKDSYLLREGIGHISSRLDPDRFVRIHRSSIVNVSR
                     IRELQPCDSGEYIAVLRDGKELSCSRGCRPQLLRLIEGGL"
     gene            complement(1441..2583)
                     /locus_tag="VEJ00_00135"
     CDS             complement(1441..2583)
                     /locus_tag="VEJ00_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXW99590.1"
                     /translation="MWTFVSLAATASVYAMYRISSQNVRLGTIAGMEFGQILSYAPLT
                     PFAYALAMRYPFRRSNWARRTLLYLAAGFVFTSCHTLLAAATPYDTWDPVNREWSSAF
                     WNPHTHSFRDPRAALKRSFLYNVVDDFSGEFVPIVLIGHAVAYYRRLRERELRTTQLE
                     GQLAKARLQTLKSQLQPHFLFNTLHSISALMLTDVVAADRMMTALSDLLRMNLESNGT
                     QLTTLSREMEFLNVYLDIEKARFEERLQVVFDIAPECLDAHVPHLLLQPLVENAVRHG
                     VSKRSSPGEIRVAARREGSGLQLWIRDNGPGLASPADEKSSHGLGLRLTRERLQTLYG
                     AEQSCDIRNLPEGGAEVSVRIPFRAVVHPAVEGDDPRLMSWGLERV"
     gene            3208..3435
                     /locus_tag="VEJ00_00140"
     CDS             3208..3435
                     /locus_tag="VEJ00_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99591.1"
                     /translation="MARSGVDSDNTFEDFFVRLHEAHDVSVYEGIERLVQAAEEVGYD
                     AKDLLRKLDQGMSFEELLELIESRMESLRAA"
     gene            3537..3872
                     /locus_tag="VEJ00_00145"
     CDS             3537..3872
                     /locus_tag="VEJ00_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99592.1"
                     /translation="MNSAVEGARTGEDEFVKEVPLAPYLSESARQAFGPALVGACLGA
                     LGGYLGSERRSAAKVLAGGLLGGAIGFGLGMIWDTREFTARVASGAWNRIRKTRDEHW
                     FEKNPIDYA"
     gene            3977..>5350
                     /locus_tag="VEJ00_00150"
     CDS             3977..>5350
                     /locus_tag="VEJ00_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXW99593.1"
                     /translation="MVGIHLSAQVASWLVPGRNLYLAIHVLGIATFLYILYRRLVPLA
                     RAQRDLRFDRPVARLGRVWKFWLGQWKHPRYRFAGTLHILIFAGFILLALRALSLLMV
                     GVSENFVIPGLSGETGRIYNFVTDYAATVVFLCMIVAAVRRLVFKPARYAVPARFGKS
                     HTPDAVFLLGLIAVLMVADSVYAASHAVRLTQQGQEAETLAFVSMPWLLQAAFGSMSV
                     ATLWGLNVGAYLVHEITFYFLLCYRPFGIQFHVETSLFSIYFAKLDRGTVKPVRWGVA
                     EENLDQVKSFGVKTFEDFTWKHMLDFYSCADCGRCADNCPANTAGRPLTPRFITIKAR
                     DYAFQHYPVFSKANNSQPLIGTIYSEDEIWSCTTCGACEEECPLLVEYIDKIVDLRRG
                     MIDDGNVPQSLQKPLKALESRGNPYGKLEKKRADWTKVAESERKIKVLDGKNGADTLY
                     FVDSITSY"
BASE COUNT         1081 a         1574 c         1612 g         1083 t
ORIGIN      
        1 cagcttggga gctccgcgtt gggctccgat aacaacgatc ttgggcacag gagcgtcggc
       61 gaaagtgtaa tcgggggtca cggtcatgcc gcccgacgta tgaatgggac tcctggattc
      121 gcttacggta aacagttcga acgggttcgg tccctgcact ccgcccacgt cctgaaagac
      181 ttcccatggt ccggcaaagt cgatcatggt ggcgccctcg gtcagaacga aggcgactcg
      241 gatcttttga ccgtctgcgg aagcaccctt ggcggcgtcc acgttcggtt gagaagcctt
      301 tgcggcacta cccccaagac cgggtgcaag cgcggcaacg atcaggcccg cgaacgctgc
      361 gaatgtcccg atcaacccgc ggtgtggctt catgctttct cctcggcgga gccaacgcta
      421 gcacgacacc ttgggcgatc cgccgcggtt gggaccagcg ccactaccta agggaccgat
      481 agcattcaag actctcccgc cggctttccg cgcgcgggag gcacccgctg agtgaagaac
      541 ccatatggca tcggcccggt ttccgtatcc gcagagccca tgctcatcgc gcttctcagc
      601 cgagcgaacc cgggctagct gaagagacaa gaccgggccc ggcaccaaat agaaagctcg
      661 acctagagtc cgccttcgat caaccggagc aattggggac ggcagccgcg gctgcaggag
      721 agttccttgc catcgcgcaa caccgcaatg tactcaccgc tgtcgcaggg ctgcaactcg
      781 cgaatccggc tgacattgac gatggaggag cggtggatgc gcacaaagcg gtccggatcc
      841 agcctggaag agatatgccc aatcccttcc cgcagtaaat aggaatcctt gcccacgtgc
      901 agctttacgt agttggccgc agcctcaatc caatccactt cattcaggtc gaggaatacc
      961 acgcgccccg cggtccggat gaccaggcgg cgcaactgcg gcgagtcagg cttgacctca
     1021 cccagcaagt cgaggatgcg cgtgcgcagg gtctgctcgt gggacttcag caattctgtc
     1081 ttgacccgct caagcgcatg gtgcaggcgc tcctgattga acggtttcag caggtaatcg
     1141 agagcatgcg cctcaaaagc ccggatggca tagtggtcga aggcggtagt gaatatgacg
     1201 aggggcattt catgggcggg gatctgcttc agcacctcga agccatccac atctggcatc
     1261 tgaacgtcca gcagcagcaa gtcgggcttg tgcgactgca ctgccgcgac cgtctgcttc
     1321 ccgtcgcgac actcggctac gacgttcact ccgctctcgg agttcagcag cacgcgcagt
     1381 ttcgtacgtg ccaatccctc gtcgtcggca atgatggtgc gtatggtcgg aacgatcatg
     1441 tcagactctc tccagccccc agctcatcag tcgggggtca tcaccttcaa ctgcagggtg
     1501 cacaacggcg cgaaatggga ttcgaaccga cacctcggcg cctccttcgg gcaggttgcg
     1561 gatatcgcag ctctgttcag caccgtaaag cgtctggagc cgctcccggg tgagtctcaa
     1621 gcccagcccg tggctggatt tctcgtcggc aggactggcc aggccaggac cgttgtcgcg
     1681 aatccagagt tgcaggccgg aaccttcgcg acgggcggcg acgcggatct cccctggcga
     1741 ggagcgcttg gacacgccat ggcgcactgc attctcgacc agcggctgca gaagaagatg
     1801 cggcacatgg gcgtcgaggc attccggggc gatatcgaat acgacttgca gccgctcctc
     1861 aaagcgcgcc ttctcgatgt ccaggtaaac gttcagaaac tccatctcgc ggctgagcgt
     1921 cgtcagctga gtaccgttgc tctccaggtt cattcgcagc aaatcgctga gggcggtcat
     1981 catacgatcg gcggcaacta cgtccgtcaa catgagggcg gagatcgagt gcagcgtgtt
     2041 gaaaaggaaa tgtggctgca gttgactctt caaggtctga agccgggcct tcgccagctg
     2101 cccctccagt tgcgtggtcc gcagttccct ttcccgcagc cgccggtaat acgccacggc
     2161 gtggccgatg agcacgatag gcacgaactc cccgctaaaa tcgtccacca cgttgtagag
     2221 gaaactgcgt ttgagcgcgg ctctcggatc ccggaacgaa tgtgtatgcg ggttccagaa
     2281 tgccgagctc cattcgcggt tcacggggtc ccaggtatcg tatggggtgg ccgccgccag
     2341 aagtgtgtga cagctggtga agacaaaccc ggcggccaga tacaacaagg tgcgtcttgc
     2401 ccagttgctc cgtcggaacg gataccgcat cgccagggcg taggcaaatg gagtaagggg
     2461 agcgtagctg agtatctgcc caaactccat tccagcgatc gtccccagcc gtacattctg
     2521 gctggatatg cgatacatcg cgtagacgct ggccgtggcc gccagtgaga caaatgtcca
     2581 cacgccgaag ctgatggccc acaggagcgg tgtgtgaatc gcgactggcg ccgacgcggt
     2641 ctcgcttcgg gtcgaatctt ccggagtcgc gacagaaacg ctccgtcgcg ggcgcggaac
     2701 aagatcccct ccgcagtgag gacacacctg cttctggctc gacgcgcatc tagggcagaa
     2761 tgtgcactca aacgaacaaa tgtatgcctc cccatcggca ggcagcgaag cctgacactt
     2821 ctggcagcgc ggtttcatct caagatgcat ggaaaaacct gatccgcaaa cggcccggcc
     2881 ggcataactc agcctgcacc ggcaaagagg caaccgggat tggagtttgt ctgctctgta
     2941 tttgacacac gagctcgtgt tttgtcatcc gttttgatcc ctcgttgcct gcaactcgta
     3001 gtcgaatgac cgccgttggt gacctgtcca gctccctccc cggtcacgat cacatcccga
     3061 aatgcgtggg attcgtcagg gaggatcaac attcccgtca cctcgaccta gatccgtggc
     3121 tgttgtgacc gcagtcacaa tatcccgtga caggcgttgc tgaccgactc cccatctgga
     3181 gttctacttt ggtaacgagg ttgggacatg gcgcgttcgg gtgtggatag tgataacacc
     3241 tttgaggact tcttcgtccg tttgcacgaa gctcacgacg tttcggtgta cgaaggcatc
     3301 gagagactgg tccaggcagc agaagaggtc ggctacgacg cgaaggatct gcttcgcaaa
     3361 ctcgatcagg gcatgagctt cgaagagttg ctcgaattga tcgagtcgag gatggaaagc
     3421 ttgagggcag cctagtcgct gcgaaggtga gtgtgcgctg cagttgatgg cgagatgggg
     3481 cgtttatgac attggcagag tggtccagat ccagcgctga ctatggtctg aagcttatga
     3541 actcagccgt cgagggggcg cgcacggggg aagacgaatt cgtgaaggaa gtgcctctcg
     3601 ctccttacct cagcgagtct gcgcggcaag cgttcgggcc ggcattggtt ggcgcctgtt
     3661 tgggcgcgtt gggtggctat ctcgggagcg agcgccggtc agcggccaag gtgttggcgg
     3721 gcggccttct ggggggagcg atcggattcg gtctcggcat gatttgggac acccgggaat
     3781 ttaccgcgcg cgtggcatcg ggcgcttgga accgcatccg caagactcgc gatgagcact
     3841 ggtttgagaa gaatccgatc gactacgcgt gagaaacgca aaggtcgttg aagtaccaca
     3901 cgcgcgccac tgactgaaca ggataagacc cctgacagct tcgtcgctga aggcaatgcg
     3961 ggaattcggg gataccgtgg taggtattca tttatccgcg caggtggcaa gctggctggt
     4021 tcccggccgg aatttgtatc tcgcgattca tgttctggga attgccactt ttctctacat
     4081 cctctacagg cgcctggtgc cgctcgctcg cgcgcagcgc gatctgcgct ttgatcggcc
     4141 ggtcgcccgt ctggggcgag tctggaaatt ctggctggga cagtggaagc atccacggta
     4201 caggttcgcc ggcacccttc acattcttat ttttgctggc ttcattctgc tggcgctgcg
     4261 cgccctctcg ctgctcatgg tgggggtgtc ggagaacttc gtgataccgg gtctctccgg
     4321 cgaaaccgga cgtatctata acttcgtcac cgactatgcg gctacggtgg tattcctctg
     4381 catgatagtc gccgcagttc gacgtctcgt cttcaagcct gcccggtacg ccgtgccagc
     4441 aaggttcggc aagagccaca cgccggatgc agtcttcctt cttggcttga tcgcggtgct
     4501 catggtagcc gacagcgttt atgcggccag ccatgctgtc aggctcacac aacaaggcca
     4561 ggaggccgag acgctggcct tcgtgtcgat gccatggctg ctccaggctg cattcgggtc
     4621 gatgtccgtc gcgacgcttt ggggcttgaa tgtcggagca tacctggtcc acgagatcac
     4681 cttctatttc ctgctctgct accggccctt tggcattcag ttccacgtgg agacttcgct
     4741 gttcagcatt tacttcgcaa agctggatcg tggcactgtc aaacccgtgc gctggggtgt
     4801 ggccgaagaa aatctcgatc aggtgaagtc cttcggcgtg aagacgttcg aagacttcac
     4861 ctggaagcac atgctcgatt tctactcctg cgccgattgc gggcgctgcg ccgacaactg
     4921 cccggcaaac acggccggac gcccactgac accccggttc atcacgatca aggctcgcga
     4981 ctacgcattc cagcactatc ccgtgttcag caaggcgaac aacagccagc cgctgatcgg
     5041 gacgatttac tcggaagacg aaatctggtc gtgcacgacc tgcggcgcct gcgaagagga
     5101 gtgcccgctg ctggtggaat acatcgacaa gatcgtcgac ctgcggcgcg gcatgatcga
     5161 cgacggcaac gtgccgcaat ccttgcagaa gccactcaaa gccctggaga gccgcggcaa
     5221 tccctacggc aagttggaaa agaaaagagc tgattggacc aaggttgccg aatcggaacg
     5281 caagatcaag gtgctcgatg gcaagaacgg tgccgacact ctctactttg tcgacagcat
     5341 cacttcctat
//
LOCUS       DASPUL010000007        10086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_412377, whole genome shotgun sequence.
ACCESSION   DASPUL010000007 DASPUL010000000
VERSION     DASPUL010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412377"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3268
                     /locus_tag="VEJ00_00155"
     CDS             <1..3268
                     /locus_tag="VEJ00_00155"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXW99594.1"
                     /translation="LPISPVASAQSDPVKERILALVVEKTGYPQDMLDLDLDLEADLG
                     VDTVKQAEMFAAIREIYTIPRDESRKLRDYPTLAHVIRFVYEKRPDLAGTVAPPMQVS
                     AAPAKEEVAVVASATALPAQPAESPASDDSITKKILQIVAEKTGYPEDMLDLDLDLEA
                     DLGIDTVKQAEMFAAVRAAYNIPRDEKLQLRNFPTLAHVIKFAHDRIGTVAPAAKTPV
                     TGRQAETKAQRPAVVSSARPVPTSFEAANRVPRRAARPILRPPLDCCKATGVTLDRKS
                     RVIVMPDKNGVAGALTQRLREEGVEVLCIDGTPDADTLTALLKNWAASGAISGVFWLP
                     ALDIEGSLAELEPAAWHEAVRVRVKLLYASMRALYEQIAGAGTFLVSGTRLGGHHGYD
                     ETGAVAPLGGGVSGFCKAYKRERPESLVKVVDFEANRSAAEIAALLIDETLRDPGAIE
                     IGYTAQNRWTIGMEEQPADDGRPGMVLDKKTVFVVTGAAGSIVSAITADLAAASGGTF
                     YLLDLVPEPDPANPDLKRFVTDKENLKRDLFTRIQARGERATPALVEKELLKLERSVA
                     AQAAIDAVHAAGGTPHYFSVDLVDHAAVAGVIEQVRLRSGRIDVLLHAAGLERSHALP
                     DKDPKEFDLVFDVKADGFFNLLHGIGDMPLGVTVAFSSIAGRFGNAGQTDYASANDLL
                     CKITSSFRTTRPATRGIAIDWTAWGGIGMASRGSIPKIMELAGIDVLPPEAGTPVVRR
                     ELTAGATHGEVVIGQRFGVLMQELDETGGLDVSAAERVLKSSTTGGPMVGKVASLCLH
                     DGYTIETTLDPRVQPFLHDHQIDGNPVLPGVMGIEAFAEAAVCLLPGWQVEAIEDVNF
                     LAPFKFYRGEPRVVRIETRIHPAGEELVADCRLVGERILSGQNEAQLKTHFTARVRLA
                     KHAAEGPQQAESVKPAGAMVGRSEIYQLYFHGPAYRVVEKAWWDGKRVVGLMARNLPP
                     NHSPAELPLAIAPRLIELCFQTAGIWEMGIQSRMGLPLRIAEVSLFRTPSPDDRALYA
                     VVTPDADRTSFNVDVLDAKGNCCLRLTDYQTVALPDAPNAERLKTLHRLMSREALLVA
                     "
     gene            3314..8902
                     /locus_tag="VEJ00_00160"
     CDS             3314..8902
                     /locus_tag="VEJ00_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXW99595.1"
                     /translation="MHNEFQRVAIVNRGEAAMRFIHAAREFRHEHDVPLRTIALFTEP
                     DRHAMFVREADEAICLGPAHVVDSDTHRPKSTYLDYKRVEQALTAARADAAWVGWGFV
                     AEHAAFADLCREMGIVFIGPSGEVMRRLGDKIAAKRLAEQSHISVAPWSNGPVETLSD
                     AFSQAERVGFPLLIKATAGGGGHGIRRVQSASQLPQAFESARSEAFKAFGDPTVFLEQ
                     LVAGSRHVEVQVIADHYGTTWAVGVRDCTIQRRHQKVLEEAPSPALTLEQDQELRDSS
                     VRLSNAAGYHNAGTVEFLYEPASRRLYFMEVNTRLQVEHPVTECTTGLDLVKLQIHVA
                     RGGRLEGDPPRTTGHAIEVRLNAEDPDNNFAAAPGTIERFRIMTGPGVRVDTGVSEGD
                     NIPSEFDSMVAKIIGYGRTRKEALSRLQRALGESVVIVKGGASNKAFLLEMLGRAEVQ
                     NADVNVGWLDRITAEDGHLSRQHADVALLIAAIDGYDAELAVEQTQFYASAARGRPQV
                     RSEIGRTLALRLRGQLYTTRTHRLSPQDYGVEVDGTRIEAHIDRRDQFESWLTVFGRR
                     FHIVSINQRISSRIEVDGVAHQVDRDDGGMVHAPAPAVVVSIAVKPGDGVEVGDRLVV
                     LEAMKMETVVVAPFPGRVRKVMTMPNVQVDVGAPLLQIEPDSGVATVGVQKRVTFGDS
                     QGSRANREATCTDSLKQLRQVMLGFDLDPALVARQFGTWSESCPASSDQITQHEDEIL
                     RIFVDICSLFHREPDVNRASAEAPSAESHLFSYLRTVENNGEGLPPAFLSALRRTLAY
                     YGVRDLHVCPELRESLLWICKSHQRMQRQANSVIDILERRLRRAKAQEPPAASSFRAL
                     LDRIVAMPIELYPSVSDLAREVRYRYFEQPAFEHERNRIYDEAENHLNYLEAHPDSAE
                     RRARLHALIECPQLLAGVFARRFPQADSSLCLTMLEALTWRYYRNCNLQNPRSQVADR
                     HSCFSVEYDEEDRHIHVFATYAEYQDMPAAARALFSLIAHVPADHDIAIDFHVCHPGK
                     LTEPEATQREVQGMINQIEFPRPIHRIVVSAASASQGARASGMQHLTFKPTEQGYEED
                     HFYRGVHPMMGKRLHLWRLSNFTIERLPSVEDVYFLHAVANDNPKDERLFAAAEVRDV
                     TPVRDESGRIVQLPHLERMLMDAFAAIRVFQSRRRPNERLCWNRVFLYVWPPLNLKPD
                     ELRDIVRRLAPAADGLGLEQVVVRARIPNPATGELRDLVVRISSPVGSGLLITFRPAD
                     RIAPLKPLTGYDQKVVRVRQRGMVYPYEIIRMLTPSPEDARTEFPPGDFVEYDLDAQG
                     KLVPVARPYGQNRANIVVGVIRNFTAEYPEGLTRVLLLGDPSKDLGALAEPECRLIIA
                     GLDLAEEKSVPVEWFPISAGAKISMDSGVENMDWIARVLRRLVEFTQGGGEVNLIVNG
                     INVGAQPYWNAEATMLMHTKGILVMTPKAAMVLTGKRALDYSGGISAEDNQGIGGYDR
                     IMGVNGQAQYWARDLNEACHILFRHYDHTYVLPGERFPRRAETSDPIGRDVQAYPHSS
                     NGEEGFASVGEILSDETNPGRKKPFDIRRVMMATVDQDHEPLERWAGMRAAETAVVWD
                     AHLGGYPICLIGIESRSVPRLGFVPADGPDQWSAGTLFPQSSKKVARAINAASSNRPV
                     VVLANLSGFDGSPESMRRLQLEYGAEIGRAVVNFKGPMVFCVISRYHGGAYVVFSQTL
                     NQQLEVAALEGTYASVIGGAPAAAVVFAGEVDARTRKNERLQAITAAMLQADAAEKGR
                     LRAQWNELYKVVYSETLGEMANEFDRVHSVFRALKVGALNHIIAPAKLRPYLIEAVER
                     GIRKEEESWRQAEERVQEVA"
     gene            8966..9685
                     /locus_tag="VEJ00_00165"
     CDS             8966..9685
                     /locus_tag="VEJ00_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HXW99596.1"
                     /translation="MNIHWLEQVEADVPMENQWLGPAETACLERLRFPKRRADWRLGR
                     WTAKQAVIAYLGLPAGPGALANIEIRAADSGAPEVFLSDQPAPVAISLSHRAGLALCV
                     LGPREATIGCDLETIESRSDAFVDDYFTPDEKTLVARAETQDRHQLVTLLWSAKESTL
                     KALRTGLRLDTTSVSVNLGQLESAGTISDYPFPLQKQLRSRGINAEGWHPLHGRYGEQ
                     VFCGWWRVELHVVRTIVSAPA"
     gene            complement(9717..>10086)
                     /locus_tag="VEJ00_00170"
     CDS             complement(9717..>10086)
                     /locus_tag="VEJ00_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99597.1"
                     /translation="AFTSKTYTFAAGPQIYIRRWQKVTFLIRPGLGGIHENANITFPA
                     GLAPLFTALQIVPPNPHQTDTEFFVGLGGGFDVNVSRKVGLRFATDWVNTHLFSNLLV
                     PRQNYVRFSVGPTFRWGHLK"
BASE COUNT         2017 a         3032 c         3098 g         1939 t
ORIGIN      
        1 cctccccatc tcacccgtgg ccagcgctca aagcgacccg gtgaaggagc gcatcctcgc
       61 gctggtggtc gagaaaacgg gatacccgca ggacatgttg gatctggacc ttgacctgga
      121 ggccgatctc ggcgtcgaca ccgtcaagca ggcagagatg tttgcggcca ttcgcgagat
      181 ctacacaata cctcgcgacg agagccgcaa gctgcgcgac tatccgaccc tcgcgcatgt
      241 gatccggttc gtctatgaga agcggcctga ccttgcgggg acggtggccc cgccaatgca
      301 ggtatcggcg gctccagcca aagaagaggt cgcggtcgta gcgtccgcga ctgctcttcc
      361 agctcaaccg gcggaatctc cggcttccga tgactcgatc acgaaaaaaa tccttcagat
      421 cgtagcggag aaaaccggct acccggagga catgctcgac ctggatcttg acctcgaggc
      481 ggacctcggc atcgacacag tgaagcaggc cgagatgttc gcggcggtgc gggccgctta
      541 caacattccg cgcgacgaga agttgcagtt acgcaacttc cctaccctgg cccacgtgat
      601 caagttcgcg cacgaccgaa taggcactgt tgcgcctgct gcgaaaacac ctgtgacggg
      661 acgccaggcc gaaacgaaag cgcagcgacc agccgtagtc tcctcagcac gtccggttcc
      721 gacaagcttt gaagcggcga atcgtgttcc ccgccgtgcg gctcgaccga tcctgcgtcc
      781 gccgctcgac tgttgtaagg caaccggcgt gacgctggat cgcaagagtc gtgtcatcgt
      841 catgcccgac aaaaatgggg tggcaggagc cttgacgcaa cgccttcgcg aggagggagt
      901 cgaagtgctc tgcattgacg gcactcccga cgcagatacg ttaaccgctc ttctcaagaa
      961 ctgggctgca tcgggtgcaa tcagcggagt cttctggctg ccggcgctcg atattgaagg
     1021 cagcctggct gaactcgagc ccgcagcctg gcacgaggcg gtgcgtgtgc gcgtgaagct
     1081 gctgtatgcg agcatgcgcg ctttatacga gcaaatcgcc ggtgcgggaa catttctggt
     1141 atctgggacg cggctcggcg ggcatcacgg ctacgacgaa actggagctg tggcaccgct
     1201 cggcggcggg gtctccggtt tttgcaaggc gtacaagcgg gaacggcctg aatcgctggt
     1261 caaagtcgtc gatttcgagg caaaccgcag cgcggccgag atcgcggcgc ttctgatcga
     1321 cgagaccctc cgtgatccgg gtgccattga gatcgggtac acggcacaaa accgctggac
     1381 tatcggcatg gaagaacagc ccgccgacga cggtcggccc ggcatggtgc tggacaagaa
     1441 aaccgtcttc gtcgtcaccg gcgctgcggg aagcattgtt tcagcgatta ctgccgatct
     1501 ggccgctgcc tcgggaggaa cgttctacct gctcgatctc gtgccggagc cagatccggc
     1561 gaaccccgat ctcaaacgct ttgtaaccga caaagagaac ttgaagcgtg acctgttcac
     1621 gcgcatacag gcccgaggcg agcgagccac tccggctctg gtggagaagg agcttttgaa
     1681 gctcgagcgc tcggttgccg ctcaggctgc cattgacgcc gtgcacgccg ccggcggaac
     1741 gccccactac tttagcgtcg acttggtcga tcacgcagcg gtggccgggg tgattgagca
     1801 agtccgcctg cgcagcggcc gcatcgatgt gctgctgcac gcggcgggtc tggaacgcag
     1861 tcatgctttg cccgacaaag atccgaaaga gttcgatctc gtatttgacg tcaaggccga
     1921 cggattcttt aacctgctgc atgggattgg cgacatgccg ctgggggtaa ccgtggcgtt
     1981 cagctcgatc gccgggcggt tcggcaacgc agggcagact gactacgcgt cggccaacga
     2041 cctgctctgc aagatcactt catcgttccg cacaacacga cctgcaacgc ggggcatcgc
     2101 gattgactgg accgcatggg gcggcatcgg catggcctcc cgcggttcga ttccgaagat
     2161 catggagctc gctggaattg acgtgctgcc gcccgaagcg ggcactcccg tggtccggcg
     2221 tgaactgact gctggagcaa ctcatggtga agttgtgatc ggccagcggt tcggcgtttt
     2281 gatgcaagaa ctggatgaga caggcggcct tgacgtatcc gccgcagaaa gggtcttgaa
     2341 gtcttcgacg accgggggac cgatggtcgg gaaggtcgcc tcgctatgct tgcatgatgg
     2401 ctacacgatc gagaccacgc ttgacccgcg ggttcagcca ttcctgcacg atcaccagat
     2461 cgacggcaac ccggtgctgc cgggggtgat gggcatcgag gcattcgctg aagcggcagt
     2521 ctgcctgctt cccggatggc aggtcgaagc aatcgaggac gtcaacttcc ttgcgccgtt
     2581 caagttctat cgcggcgagc cgcgcgtcgt gaggatcgaa acgagaattc atcctgccgg
     2641 agaggagctg gtcgcggact gccgtctggt cggcgaacgc atcctgtccg ggcaaaatga
     2701 agctcaactg aagacgcact tcaccgcccg cgttcggctg gcgaagcacg ctgccgaagg
     2761 gccgcagcag gcagagtcgg taaaacctgc cggagcaatg gtcggccgct cggagatcta
     2821 ccagctctac ttccacgggc cagcgtaccg ggtggtggag aaagcctggt gggatggaaa
     2881 acgcgtcgtg ggtttgatgg caagaaacct gccgccgaat cattctccgg cagagttgcc
     2941 gctggcgatt gctccacgac tcatcgaact gtgcttccag accgccggga tctgggagat
     3001 gggtatacag agccgtatgg gcctgccgct gcgcatcgcc gaagtttcat tatttcgtac
     3061 acccagcccg gatgacaggg cgctgtatgc cgtcgtcacg cctgatgcag accgaacaag
     3121 cttcaacgtc gatgtattgg acgcgaaagg caactgctgc ttgcgtctca cggactatca
     3181 gacggttgcg ctcccggatg ctccaaacgc ggagcgactg aagaccctac atcgcctcat
     3241 gtcaagagag gctcttctcg tggcctaacg aattgcaggc cactttgtca ccgactgttt
     3301 ctacggggca tttatgcaca atgaattcca acgcgttgcg attgtcaatc gcggcgaagc
     3361 tgccatgcgg ttcatccatg cggctcggga attccgccac gagcacgatg ttcccctccg
     3421 cacgatcgcc ctgttcacag aaccggaccg ccatgccatg tttgtccgtg aggccgacga
     3481 ggctatttgt ctcggccctg cccacgtcgt ggactcagac acgcatcgtc ccaagagcac
     3541 ttacctcgac tacaagcggg ttgaacaggc gctgactgcc gcgcgggcag acgcagcatg
     3601 ggtcggatgg ggcttcgttg cggagcatgc ggcattcgcc gatctctgtc gtgagatggg
     3661 gatcgtcttc atcggtccca gcggcgaggt catgcggcgg ctcggtgaca agatcgccgc
     3721 caaacgtctt gcggaacaat cacatatctc cgtcgcaccc tggagcaacg ggcccgtgga
     3781 gactctctcg gacgctttct cccaggcgga gcgtgtggga tttccgcttc tgatcaaggc
     3841 gacggcggga ggcggcggcc atggaattcg ccgtgtgcaa tccgccagcc agctgccgca
     3901 agcttttgag agcgcccgct ccgaggcctt caaggctttc ggagacccta ccgtctttct
     3961 tgagcagcta gtggccggtt ctcgtcacgt ggaggtccag gtcatcgccg accactacgg
     4021 aaccacgtgg gcggtgggtg tgcgtgattg caccatccag cggagacatc agaaagttct
     4081 cgaagaagct ccgtctccgg ctctcactct cgagcaagat caagaactgc gagattcctc
     4141 agttcgcctg agcaacgccg cgggctacca caacgccggt acggtggaat ttctttatga
     4201 gccagcaagc cggcgcctct acttcatgga agtgaacacc cgcttgcagg tcgagcatcc
     4261 ggttaccgaa tgcactaccg gactcgatct ggtgaagctt cagattcacg tagcccgcgg
     4321 aggacggctg gaaggagatc ctccgcgcac cacgggtcac gcgatcgaag tgcgcctgaa
     4381 tgcggaggat cctgacaaca acttcgcggc ggcgcccggc accatcgagc gcttccggat
     4441 catgaccggt ccgggcgtgc gcgtcgacac cggcgttagc gagggcgaca acattccctc
     4501 ggaattcgac tccatggtcg ccaaaatcat cggctacggc cgaacgcgga aggaagctct
     4561 gtcgcggctc cagcgggcgc tgggcgagag cgtcgtaatc gtcaagggag gagcgagcaa
     4621 taaggcgttc ttgctcgaga tgctgggccg cgccgaggta cagaacgcag acgtgaatgt
     4681 cggctggctc gaccgcatca cggccgagga tggccatctt tcccgtcagc acgcggacgt
     4741 agcccttctg atagccgcca tcgacggata cgatgccgag ttggccgtgg aacaaactca
     4801 gttctacgct tcagccgcgc gcggccggcc gcaggttcgc agcgagatcg gccgaacgct
     4861 ggcactgcgt ctccgcggcc agctctacac cacgagaacg caccgcctga gcccgcaaga
     4921 ctacggggtg gaagtggacg gaacgcggat tgaggctcac atcgaccgcc gggatcagtt
     4981 cgagtcctgg ctcactgtct tcggccgccg ctttcatatc gtctcaatta accagagaat
     5041 tagttcccgg attgaagtag acggcgtggc gcaccaggtc gaccgagatg acggcgggat
     5101 ggtacacgct ccggctcctg ctgtggttgt ctcgatcgcc gtgaagccgg gtgacggggt
     5161 ggaagtcggc gatcgcctgg tggtactcga agcaatgaag atggagaccg tggtggtcgc
     5221 accttttccg ggacgagtgc ggaaggtaat gaccatgccg aacgttcagg tcgacgttgg
     5281 cgctcccctt cttcagattg agcctgatag cggtgtagca acagtcggag tgcaaaagcg
     5341 cgtcactttc ggcgactcgc agggctcccg cgcgaaccgc gaggcgacgt gcacggacag
     5401 cctcaagcag ctgcggcagg tgatgctcgg tttcgacctc gatccggcac tggttgcgag
     5461 gcagtttggc acatggagcg agagttgccc ggctagcagt gatcaaatca cgcagcatga
     5521 ggacgaaatc ctccgtatct tcgtggatat atgctccctg ttccatcgcg agccggatgt
     5581 caaccgcgct tccgcggaag cgccgagcgc tgagtctcac cttttttcgt atctgcggac
     5641 ggtcgagaac aatggggaag gtctgccccc ggcctttctg agtgcgctgc ggagaactct
     5701 cgcttactac ggcgtacgcg atttgcacgt ctgccccgaa ttacgggaaa gcttgctctg
     5761 gatctgcaag tcgcatcagc gcatgcaacg gcaggcgaac tctgttatcg acatccttga
     5821 gcgccgccta cgccgcgcca aggcccagga accgcctgct gccagttcat tccgtgctct
     5881 cctggatcga atcgttgcca tgccgatcga gctctaccct tcggtgagcg accttgccag
     5941 ggaagtacgg tatcgctatt ttgagcagcc ggcgttcgaa cacgaacgga accgcatcta
     6001 cgacgaagcg gagaatcatc tcaactatct cgaggcccat cctgactcgg cggagcgccg
     6061 ggcccggctg cacgcgttga tcgaatgtcc tcaattgctc gccggtgtat ttgcccggcg
     6121 cttcccacaa gcggattcct ctctttgcct gaccatgctg gaggctctca cctggaggta
     6181 ctatcgcaat tgcaatttgc agaacccacg ctcccaggtc gcagaccggc attcttgctt
     6241 ttcggtggaa tatgacgaag aagacaggca catccatgtg tttgccacat acgctgagta
     6301 tcaggacatg ccggcagctg cgcgggcttt attctccctg atcgcgcacg tgccggctga
     6361 tcacgacatc gcgatcgatt ttcatgtttg ccacccggga aaactcaccg agccggaggc
     6421 tacacaacga gaagtgcagg ggatgatcaa ccagattgag tttccccgtc cgatccaccg
     6481 catcgtggtg tctgctgctt ctgcgagcca aggggcgcgc gccagcggga tgcagcacct
     6541 cacgttcaag cctaccgagc aagggtatga ggaggaccat ttttatcgcg gtgtgcatcc
     6601 gatgatgggc aagcgccttc atctgtggcg gctcagcaat ttcacaatcg agcgcctgcc
     6661 ttcggtcgag gacgtctatt tcctgcatgc cgttgccaac gacaacccca aggacgagcg
     6721 cctcttcgcc gctgccgagg tgcgcgatgt caccccggtg cgcgatgaat cgggacggat
     6781 tgtgcagttg ccgcatctgg aacgcatgct catggatgcg ttcgctgcga ttcgcgtgtt
     6841 ccagtcgcga cgacgcccga acgagcgcct atgctggaat cgggtctttc tatatgtctg
     6901 gcccccgctt aatctgaagc cggacgaatt gcgtgatatc gttcgccgcc tggcgccagc
     6961 cgcggacggc ctgggcctgg aacaggtcgt agtacgggct cgcatcccaa atcccgctac
     7021 tggagaattg cgcgacctgg tcgtccgcat ctcgagcccg gtgggaagcg gtttgctgat
     7081 caccttccgc cccgccgacc gaatcgcgcc tctcaaaccg ctgactgggt acgatcaaaa
     7141 agtggttcgc gttcggcagc ggggcatggt ctatccctac gaaatcatcc ggatgctcac
     7201 tccatcgccg gaagacgccc gaaccgaatt cccgccgggc gatttcgtcg aatacgatct
     7261 cgacgcccag ggaaaactag tcccggtcgc gcgcccctat ggacagaatc gcgccaacat
     7321 cgtcgtcggg gtcatccgca acttcaccgc ggaatatccc gaaggcctga cccgcgtgtt
     7381 gctgctggga gatcccagca aagatcttgg agcgctcgcc gaaccggagt gccggctcat
     7441 catcgccggg ctggatctgg cggaggagaa gtccgtccca gtcgagtggt tcccgatttc
     7501 ggccggcgcc aagatttcaa tggacagcgg cgtcgaaaac atggactgga tcgctcgcgt
     7561 attgcgccgg ctggtggaat tcacgcaggg cgggggcgag gtgaatctga tcgtcaacgg
     7621 cattaacgtc ggagcccagc cgtactggaa cgctgaggcc acaatgctga tgcacaccaa
     7681 aggcattctg gtcatgaccc cgaaagcagc gatggtgctt acgggcaagc gtgcgctgga
     7741 ctattccggc ggtatttccg cagaagacaa tcagggaatt ggtggctatg accgcatcat
     7801 gggagttaac ggccaggcgc aatactgggc gcgcgacctg aatgaagcct gccacattct
     7861 tttccgccat tacgaccata cttacgtcct gcccggagag cgcttccccc gccgcgccga
     7921 gacttccgat ccgatcgggc gcgatgtgca ggcctatcct cacagtagca atggtgaaga
     7981 agggtttgcg agtgtgggcg aaatcttgtc agacgaaacc aaccccggac ggaagaagcc
     8041 attcgacatt cgccgggtga tgatggcaac ggtcgatcag gatcacgagc ctctggagcg
     8101 ctgggcggga atgcgcgcgg ccgagactgc cgtagtgtgg gatgcgcatc ttggcggcta
     8161 ccccatctgc ctcatcggca tcgaatcccg ttcggtgccc cgtctgggat tcgtgcccgc
     8221 cgacggcccc gatcaatggt ccgccggcac gctcttcccg cagtcttcca agaaagtggc
     8281 gcgcgccatc aatgccgcga gcagcaatcg cccggtggtg gtgctcgcca atctctccgg
     8341 tttcgatggt tcgccggaat cgatgcgcag gctgcagctg gaatatggcg ccgagatcgg
     8401 aagagcggtc gtgaacttca aaggccccat ggtattttgt gttatatctc gctaccatgg
     8461 cggcgcctat gtggttttct cgcagacgct gaaccagcag ctggaggtag cggccctgga
     8521 aggaacctat gcttccgtaa ttggcggggc cccggccgcg gccgtcgtgt ttgccgggga
     8581 ggtcgatgcg cgtacccgca aaaacgagcg cctgcaggca atcacggcgg cgatgctgca
     8641 ggccgacgcc gccgagaaag gccggctgcg ggcgcagtgg aacgaactgt ataaggtcgt
     8701 ctattccgag acgctgggcg aaatggccaa tgagtttgac cgcgtacaca gcgtgttccg
     8761 ggcactcaag gtgggagcgt tgaaccacat cattgcgccg gcgaagcttc gcccctatct
     8821 catcgaagcc gtggagcggg gtatccgaaa agaggaagaa tcctggaggc aggcagaaga
     8881 gcgagtccag gaggtcgctt aagatcctgg tcgtcccgat ccgcggttcg tatgtgggtc
     8941 aactgatcct ctgtcgaaga gcgggatgaa catccactgg ctggaacaag tcgaagccga
     9001 tgtgcccatg gaaaatcaat ggctcggtcc ggccgagacg gcgtgtctgg agcgcctgcg
     9061 ctttcccaag cggcgcgcgg actggcgcct cggccgctgg acggcgaagc aagcggtaat
     9121 cgcctacctc ggcctgcctg ccggccccgg cgcgcttgcg aacatcgaga tacgggccgc
     9181 cgattccggg gcgccagaag tgtttctctc cgatcaaccc gcgcctgtcg cgatctccct
     9241 cagccaccgc gccggcctcg cgctgtgcgt tctcggtccc cgagaggcca cgatcggctg
     9301 cgatctggag acgatcgaat cccgcagcga tgcttttgta gacgattact tcacgcccga
     9361 cgaaaagact ctcgtcgcac gggcagaaac gcaggatcgg catcaacttg tgaccctgct
     9421 atggagcgca aaagagagca cgctcaaggc tctccgcacc ggacttcgac tcgacactac
     9481 cagcgtgtcg gtgaatcttg gccagctcga gtccgctgga acgatcagcg attatccttt
     9541 tccactgcaa aagcaacttc gttcccgagg cattaacgcc gagggttggc accccttaca
     9601 cggtcgctat ggtgaacagg ttttctgcgg ctggtggcgc gtcgagctcc acgttgtcag
     9661 gaccatcgtt tctgccccgg cttagccgag aacccagtct gctctgcact ccagcgctat
     9721 ttcaagtgcc cccagcgaaa agtcgggcct accgagaaac ggacatagtt ctgccgtgga
     9781 accagtaagt tcgagaacag gtgagtgttc acccagtcgg tcgcaaagcg caggcccact
     9841 ttccgcgata cgttgacatc gaagccgccg cccaaaccaa cgaagaattc cgtatctgtc
     9901 tgatgcggat tgggcggaac aatctgtaga gccgtgaaca ggggtgccag acccgcgggg
     9961 aaagtgatgt tggcgttttc gtgaatgccg cctagacccg gacgaataag aaacgttacc
    10021 ttttgccacc tacgaatgta gatctgcggg ccagcagcaa acgtgtatgt tttcgacgta
    10081 aacgca
//
LOCUS       DASPUL010000008         7013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1783242, whole genome shotgun sequence.
ACCESSION   DASPUL010000008 DASPUL010000000
VERSION     DASPUL010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783242"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VEJ00_00175"
     CDS             complement(<1..327)
                     /locus_tag="VEJ00_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXW99598.1"
                     /translation="MRKIMLLLVCVLLALPVIGQTTLNGAGATFPYPMYSKWFSEYHK
                     LHSDVQINYQSIGSGGGIRQVTEGTVDFGASDMPMTDDQLREAEGKVKTKVLNIPTVL
                     GAVVPAY"
     gene            complement(636..1427)
                     /locus_tag="VEJ00_00180"
     CDS             complement(636..1427)
                     /locus_tag="VEJ00_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99599.1"
                     /translation="MARAEDFARVAALSPLDRGFSGLYNLDFAGAQKDFAYWQNEHPD
                     DPVGPVSEAAGYLFSEFNRLGVLESQFYADDGAFSARPKLTPDPAVRERFQKALGRAE
                     ALAHARLAKDPKDRDGLFALTMSSGLRADYAALIEKKNMASLHFTREATASAQQLLEV
                     CHDCYDALLATGFSKYIIGSMSAPVRWILRMGGLPADKQGGIADLETTAEHGHYLAPF
                     ARILLAIAYVREKDKTRALQLLTDLQREFPGNTLFPREISHLSTH"
     gene            1674..4085
                     /locus_tag="VEJ00_00185"
     CDS             1674..4085
                     /locus_tag="VEJ00_00185"
                     /EC_number="3.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004339 - glucan 1,4-alpha-glucosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009251 - glucan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan 1,4-alpha-glucosidase"
                     /protein_id="HXW99600.1"
                     /translation="MTDSPVRYAPGWPGIPPRWTSSAKSGAGTALNQHSKVWFTVSHG
                     ILNEVYFPRVDQACTRDMGFIVTNGSDFFSEEKRHCSFENMPLEPGIPALELINTCDS
                     GRYRIHKEVLTDPYRNVVLQKVRFEPLQGKLSDYHLYALLSPHLANCGYGNTGWIGDY
                     KGYPMFFAEHDGVTLSIAASCPWKKMSVGFVGASDGWQDLNQHYQMEWEYDRAENGNI
                     AFTGEIDLAASNGEFVLALGFGGIWTEAGQQARSALFEDYAEIREHFVKQWSDWQSKL
                     LTLDKPQRQYDLYRTSTAVLRTHESKDFLGGIIASLSIPWGFNKGDEDLGGYHLVWPR
                     DLVETAGALLAAGATNDAVRVLRYLEATQEAAGNWAQNLWLDGRPYWSGVQMDETSFP
                     ILLIDLLRREAPGALGRLDRWWPMVRNAASFILRNGPVTQQDRWEEDGGYSPFTLAAE
                     ISALLAAADIADLTGHSEQAQTMRDTADAWNANIERWTYATGGDLAQQLGIEGYYVRI
                     APPDTDGAASPTQGFVPIKNRPPGQDRERAYHIISPDALALVRFGLRAPDDRRILNTL
                     RAIDALLSVELPQGPCWYRYNGDGYGEHKDGSPFDGTGIGRAWPLLAGERAHYALAGG
                     HRDEAEALLTVMENSTAGASRLLPEQVWDAADIPALELFRGKPSGSACPLVWAHSEYV
                     KLRRSIRDGKVFDQPPQTVQRYLVEKHGRQHYGWRINNKARTVPYKETLRINLLSPAL
                     VHWSIDGWKTTNDTKTHDTGLGLYTVNLPTASLPVGGQVVFTIYWQEEQRWEGKDYTV
                     TIEPS"
     gene            complement(4152..6791)
                     /locus_tag="VEJ00_00190"
     CDS             complement(4152..6791)
                     /locus_tag="VEJ00_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXW99601.1"
                     /translation="MIGQTISHYRIVEKLGGGGMGVVYKAEDTELGRFVALKFLPEDL
                     ARDPQALERFRREARAASALNHPNICTIYEIGKHGEQMFIAMEFLDGVTLKQRILGRP
                     LDLDLLLPAAIEIADALDAAHAAGIIHRDIKPANLFLTTRGHTKILDFGLAKLHVAST
                     ASGSESAVTLDDNLTSPGTALGTVAYMSPEQVRGKDLDRRTDLFSFGAVLYEMATGLL
                     AFRGDTSGIVFDEILNREPAAIVRLNPSVPPELERIIHKALEKDREIRYQSAAEMRVD
                     LKRLRRDTESGRALVTSSGSEVVTSTAATAAVSARRPRRVWAIPAALVCALLAWAVIS
                     AFLPPTVPHITRTTQITNDGLAKTGFVTDGNRLYFNEMVAGKSVLAQASTAGGETSQT
                     PMPWPESWIIDISPRGNELLIGKEGRAPGQFALWILPLPAGSPRRVGALETSGSASWL
                     PDGEHIAFVQKRSEVYRARTDGTEVQRIFSRPDIGNVVFSPDGRHICFNVSDPQTDST
                     TIWEARTDGSDAHRLLSAGWNKPEREYVGNWTADGKNILFLSRRDRLTEIWLLPESRG
                     WLFKRTPEPVRLTSGPISYLSPVPSKDGKQLFVLGSQARSELVRYDSKSGQFLPFLSG
                     ISAGHVEFSRDGKWVTYVSYPENTLWRRRADGSEAQQLTYPPLLVVQPHWSPDGTRIA
                     FTGIEPDKPWRIYIVPAGGGAIEPLLVENRSQLDPVWSPDGNSIIFGRIESREEKLNL
                     QLIDLKTRKLSDIPDSEGRWVPSWSPDGNKLVAVDRTDEILSVFDFKTQKWSELARST
                     EQITDVHFSHDGKAIYYQDFGDNAVHRVPLTGGKVETVLSLKDLRRPDLPYWPPWMGL
                     GPDDSILVMRDTGSQEIYALDWE"
BASE COUNT         1369 a         2213 c         2085 g         1346 t
ORIGIN      
        1 gtaggcaggc acgaccgcgc ccagcacggt aggaatgttc agaaccttcg tcttgacctt
       61 gccttcagcc tcgcgtaact gatcgtcggt catgggcatg tcgctggccc cgaagtcgac
      121 cgtcccttca gtcacctggc gaatgccgcc gccgctgcca atcgactggt agttgatttg
      181 aacatcggaa tgcagcttgt ggtactcgct gaaccacttc gagtacatcg gataggggaa
      241 cgtagcgcct gctccgttca gcgtggtctg cccgatcact ggcagggcca gtaggacgca
      301 caccaacagc agcatgattt tgcgcatcag cgaatgactc ctccttgtaa ttgatgtccc
      361 cgttgtatcg gcgcagtgtt acaggatggt tacaaacggg taaatttctg atgaacaccc
      421 ccccgacgct ctaaaccaat ctgctggccc agatgaaacg ttttcaacct gcctttgacg
      481 acgacactcg tgactgctca gcagagcgtc agggtgctcg cgcttttatc gtgagagcgg
      541 agtttgcagc ctgttgcggc cgcgaacgga aaagtcataa ttaggagtcg acgtcggacc
      601 gcagtcaggc aaggtagaac cgaaggcgag gcggactagt gtgtggacag atgcgaaatt
      661 tcgcgtggga acagagtgtt tcccggaaac tcacgctgca ggtcggtcag caattgcagg
      721 gcccgcgtct tgtctttctc ccgaacgtag gcgatggcga gcaaaatgcg cgcaaacggc
      781 gccaggtagt gcccgtgctc tgcagtggtc tcgagatcgg cgattccgcc ctgcttgtcc
      841 gccggcaacc cgcccatacg caaaatccat cgcaccggcg ccgacatgct gccgatgatg
      901 tacttgctga agccggtggc cagcagcgcg tcgtagcaat cgtggcagac ctcgagaagc
      961 tgctgcgcag aggcggtagc ctcccgggta aagtgcagcg aggccatgtt cttcttttcg
     1021 atcagcgccg cgtaatcggc ccgcaagcct gaagacatcg tcagcgcaaa caggccgtcg
     1081 cgatccttcg ggtctttcgc aaggcgtgcg tgcgcgagag cctccgcccg cccgagcgcc
     1141 ttctggaaac gttcccgcac ggcaggatcg ggcgtcagct tcggccgcgc cgaaaacgcg
     1201 ccatcatcgg cgtaaaactg cgattccagc actcctagcc ggttgaactc ggagaacagg
     1261 tatcctgctg cctcactgac cggacccacg ggatcatccg gatgctcatt ctgccagtag
     1321 gcaaagtctt tctgcgcgcc cgcgaaatca aggttgtaga gtccggagaa tccgcgatcc
     1381 agcggagaca gggcagcgac gcgggcaaaa tcctccgctc tcgccaaccc tcccgcgaca
     1441 cacaccaccc acaagactgc tactattttc agcgctcgtg catcagagag tcttgagtgg
     1501 gccatgtttc cgaagcgagc ttgttcatta tcgccaatct gctcgcaggt gcaagtggtc
     1561 cctggcattg tgtccgccta ttttgatgcc atcagtcaag gaagcagcat gcaatcctgt
     1621 caaacttttg taaattccag cgccggcagc ctccgccgag ggacgatccg gatatgaccg
     1681 attcgcctgt tcgttacgct ccgggctggc ccggcattcc cccgcgctgg acctcgagcg
     1741 ccaagagcgg cgccggtacg gcgctcaacc agcacagcaa ggtgtggttc acggtgagcc
     1801 acggcatttt gaacgaggtc tactttcccc gtgtcgacca ggcctgcacc cgcgatatgg
     1861 gatttatcgt caccaacggc agtgactttt tctccgaaga gaaacgccat tgctcgtttg
     1921 agaacatgcc cctggagccc ggcattccgg ctcttgaatt gatcaacacc tgcgacagcg
     1981 gccgttatcg catccacaaa gaagtcctca ccgatcccta ccgaaacgtc gtcttgcaga
     2041 aagtccgctt cgaaccgttg cagggcaaac tttccgacta ccacctttac gccctgctct
     2101 ctccccacct cgccaactgc ggatacggca acaccggatg gattggcgac tacaagggct
     2161 atcccatgtt cttcgccgaa catgacggcg tcactctgtc catagctgct tcttgcccgt
     2221 ggaagaagat gtcggtgggt ttcgtcggcg cctccgacgg ctggcaggat ctcaaccagc
     2281 attatcagat ggaatgggaa tacgaccgcg ccgaaaacgg caacatcgct ttcaccggcg
     2341 agattgatct tgcggcctcg aacggcgagt ttgtgctggc tctcggtttc ggcggcatct
     2401 ggaccgaggc cgggcaacag gcccgctcgg ctctgttcga agactacgcc gagattcgcg
     2461 agcacttcgt caagcagtgg agcgactggc agagcaagct gcttacgctt gacaagccgc
     2521 agcgccagta cgatctctac cgcacgtcga ctgcggtttt gcgcacccac gaatcgaagg
     2581 attttctcgg cggcatcatc gccagcctct ccattccctg gggattcaac aaaggcgacg
     2641 aggatctcgg aggctaccac ctggtctggc cgcgcgatct ggtcgagacc gccggcgcgc
     2701 tgctcgcggc tggagcaacc aacgacgcgg ttcgcgttct tcgctacctt gaagccacgc
     2761 aggaagccgc cggcaactgg gcgcagaatc tctggctcga tggccgcccc tactggagcg
     2821 gcgtgcagat ggatgaaact tccttcccca tcctgctcat cgacctgctc cgccgcgagg
     2881 cccccggcgc gctgggcagg ctcgatcgct ggtggccgat ggtgcgtaac gccgccagtt
     2941 tcattctgcg taacggcccc gtcacccagc aggaccgatg ggaggaagac ggcgggtatt
     3001 cgccgttcac tctggctgcc gaaatttccg ccctgttagc ggcggccgac attgccgatc
     3061 tcacgggcca cagcgagcag gcgcaaacca tgcgtgacac cgccgacgcc tggaatgcca
     3121 acatcgagcg ctggacctac gccaccggag gcgacctggc gcagcagctt ggaatcgagg
     3181 gctactatgt tcgcatcgcc ccgcccgaca ccgacggcgc cgcctctccc acacagggct
     3241 tcgtgcccat caagaatcgt cctccgggcc aggatcgcga gcgcgcctat cacatcatca
     3301 gccccgacgc gctggccctg gtgcgcttcg gcctgcgcgc acccgacgac cgccgcattc
     3361 tgaatacgct gcgcgccatc gatgccctgc tcagcgtcga gttgccgcag gggccgtgct
     3421 ggtatcgcta taacggggac ggctacggcg agcacaagga cggctctccg ttcgacggca
     3481 ccggcattgg ccgcgcctgg cccctgctgg cgggagaacg cgcccactat gccctcgccg
     3541 gcggacaccg cgacgaagcc gaagccctgc tcaccgtcat ggaaaattcc accgccgggg
     3601 ccagccgcct gctaccggag caggtttggg acgccgctga catccccgct ctggagcttt
     3661 ttcgcggcaa gccttcgggc tcggcctgcc cgctggtgtg ggcgcactct gaatacgtca
     3721 aacttcgccg ctccatccgc gatggcaagg tctttgacca gccgccgcag accgtgcagc
     3781 gctacttggt agagaagcat ggccggcaac attacggctg gagaatcaat aacaaggcgc
     3841 gcaccgtccc ctacaaggag accttgcgca tcaacctgct ctcgccggcg ctggtgcact
     3901 ggagcatcga cggctggaaa accaccaacg acaccaagac ccacgacacc ggcctcgggc
     3961 tctatacggt caatcttccg accgcctcgc tcccggtcgg cggtcaggtg gtctttacga
     4021 tctactggca ggaagagcag cgctgggagg gcaaggacta cacggtcact atcgaaccat
     4081 cgtgacgacg cgatcgcccg cggctcttcg accccactaa aaggttgcgc agcgactggc
     4141 gcttggcgcg cctactccca gtcgagggcg tagatttcct gactgccggt gtcacgcata
     4201 acgagaatgg aatcgtcagg gccaagaccc atccagggtg gccagtacgg caggtcgggg
     4261 cgccggaggt ccttgagaga caggaccgtt tccacctttc ccccggtgag aggcacgcgg
     4321 tggactgcat tgtcgccgaa atcctgatag tagatggcct tgccgtcgtg cgagaagtga
     4381 acgtcggtga tctgctccgt gctgcgggcg agttcggacc atttctgcgt tttgaaatcg
     4441 aaaacggaga ggatttcgtc ggtccgatcc acggcgacta gcttgttgcc atcaggcgac
     4501 catgagggaa cccatcgtcc ctctgaatcg ggaatgtctg acagttttct tgtcttcagg
     4561 tcgatgagtt gcagattgag tttttcttcc cggctctcga tccggccgaa gatgatggaa
     4621 ttgccgtccg gtgaccagac agggtcgagc tggctgcggt tttccaccag caggggctcg
     4681 atcgctccac ccccggccgg aacgatatag atgcgccagg gcttgtcggg ctcgatgccg
     4741 gtgaacgcaa tccttgtacc gtcaggagac cagtgcggct gcaccaccag caggggaggg
     4801 taagtcaact gctgggcttc gctgccgtcg gcgcgccgcc tccacaaggt gttttcaggg
     4861 tagctaacat aggtcaccca ttttccgtcc cgcgagaatt ccacgtgccc ggcggagatg
     4921 ccggagagaa acgggagaaa ctgtcccgac ttgctgtcat aacgcaccag ttcagagcga
     4981 gcctgcgaac ccagtacaaa cagctgcttg ccgtccttgc tgggaaccgg agagaggtac
     5041 gagatcgggc cgctggtcag gcgcaccggc tccggcgtgc gtttgaagag ccatccccgg
     5101 ctttcgggca gcaaccatat ctcggtcagg cgatcgcgcc ggctgaggaa gagaatgttc
     5161 ttcccgtccg cggtccagtt gcccacgtac tcgcgctccg gcttgttcca tcccgcggaa
     5221 agcaaccgat gcgcgtcgct gccgtcggtc cgggcctccc agatcgtcgt actgtcggtc
     5281 tggggatcag aaacattgaa gcaaatgtgg cgcccatccg gcgagaacac gacgttgccg
     5341 atgtcgggcc gggaaaagat tctctggact tcggtgccgt cggttcttgc ccggtagacc
     5401 tcggaccttt tttgcacaaa ggcgatgtgt tctccatcag gcaaccaact ggcactgccc
     5461 gacgtttcca gcgccccgac gcggcgtggc gacccggccg gtaagggaag aatccacagc
     5521 gcaaactgcc cgggtgctct gccttccttg cctatcagga gctcgtttcc gcgaggagag
     5581 atgtcgatga tccaggactc tggccacggc atcggagtct gggaagtctc gcccccagcg
     5641 gtcgaggcct gtgccagtac actcttaccc gctaccattt cgttgaaata caaacggttg
     5701 ccgtctgtga caaaaccggt tttagcgagg ccatcgttcg taatctgcgt ggtccgcgta
     5761 atgtgcggga ccgttggcgg cagaaacgcg ctgatcactg cccacgccag caatgcgcat
     5821 accagagcgg cagggatcgc ccagacccgg cggggacggc gcgcggacac cgcagcagtt
     5881 gcggcggtgg atgtcacaac ctccgagccg ctgctggtga ccagcgcgcg gcctgactcc
     5941 gtgtcgcgac gcaggcgctt caggtccacg cgcatctcgg cggcactctg atagcggatc
     6001 tcacgatcct tttcgagcgc cttgtggata atgcgctcca gctccggcgg caccgagggg
     6061 ttcagacgca cgatcgcggc cggttcacgg ttcagaatct cgtcgaagac gattcccgac
     6121 gtgtccccgc gaaaggccaa aagacccgtc gccatctcat agaggaccgc cccgaatgag
     6181 aacagatcgg tgcgccggtc cagatccttt ccccgcacct gctcgggcga catgtaggcg
     6241 accgtgccca acgctgtgcc cgggctggtg aggttgtcgt cgagcgtgac ggcagactct
     6301 gagcccgacg cagtcgacgc aacatgcagc ttggccaggc cgaagtcgag gatttttgtg
     6361 tgaccccggg tggtgagaaa cagatttgcg ggcttgatgt cgcggtgaat gatgccggcg
     6421 gcgtgggccg cgtcgagcgc atcggcgatt tcgatggccg ccggcaacaa aaggtccaga
     6481 tccagcggac gccccaaaat gcgctgcttc agcgtaacgc catcaagaaa ctccatcgcg
     6541 atgaacatct gctcgccatg cttgccaatt tcgtagatgg tgcagatgtt gggatggttc
     6601 agggcagatg ctgcacgggc ttcgcggcga aaccgctcca gcgcctgcgg gtcgcgggcg
     6661 aggtcctccg gcaggaactt cagggcaacg aagcgcccca gttcggtgtc ctctgccttg
     6721 tagacgactc ccataccacc gccgccgagt ttttccacga tgcggtagtg cgagatggtc
     6781 tggccgatca ttggcgggaa tcttacgatc acccaaaagg cgagtcaatg aaacacgcga
     6841 cgatccgcgg ccgcgacgga accgggcgaa gtcaaattct ccgcccgtcg tgcgtcctga
     6901 tcattcgccc aacggtgcat tgcgcagcct tcctctctgg gcgacgcctg cacattcaga
     6961 cagcccgcac ggtcaaggag tatgctctat gtcgcacttt caattgtgag ggg
//
LOCUS       DASPUL010000009         3872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_549584, whole genome shotgun sequence.
ACCESSION   DASPUL010000009 DASPUL010000000
VERSION     DASPUL010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549584"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /gene="rsmG"
                     /locus_tag="VEJ00_00195"
     CDS             complement(<1..524)
                     /gene="rsmG"
                     /locus_tag="VEJ00_00195"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HXW99602.1"
                     /translation="MDPARIAALLEPFLSAKLSPSCRSERASAREKPDVLSPAQLRGI
                     SIYIDLILRWNARINLTAIRDPEEIVTRHFGESFFAARILFPAFSANSAAKGVHVADV
                     GSGAGFPGLPIKLWAPHVSLTLIEPNHKKATFLRETVRALSLTNVNVVTYRAEELLKH
                     SPPTSFDVVTLRAV"
     gene            complement(803..2437)
                     /locus_tag="VEJ00_00200"
     CDS             complement(803..2437)
                     /locus_tag="VEJ00_00200"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXW99603.1"
                     /translation="MAKTRPYLFYDIAISICSTCYRRVDAKIVFQDTNVYLLKRCPDH
                     GPERILIADDVDYYRRCREVFIKPPEMPLVYNTPVKWGCPYDCGLCTDHEQHSCLTLV
                     EICDYCNLRCPVCYASSGPERQQFRDLKQIEKMLDAVVRNEGHPDVVQLSGGEPTVHP
                     QFFEIVELAKARPIKHLMVNTNGVRIAQEEDFVQRLAEIKEDFEVYLQFDSFERDPLM
                     ELRGADLRRIREDAIEKLNRYNISTNLVVTLKKGLNDHEIGKTIDYALQQPCVRGVTF
                     QPIQDAGRLETWAPNRVGTDAFVRPPDVLTDSGAPLLPDVGRSGGFDGNGGYVHNFNP
                     ATDRLTLTEVRRKILEQTQVFRPEDVIPVPCHPDSLAMAYALKLNGRVTPLTSMIPPE
                     VLINGAKNTILYEQEPSVRDNLFKLFATNHSPQSSAGTLRELLCCLPPVWIPNSPQQS
                     CHSEPARSGGEEPAVRSLTYENIFRVIIMQFLDAHSFDVRSVKKTCVHIVHPDGRLIP
                     FDTYNLFYRDDLEQTRLDPLKRVADPALAPAYEIAR"
     gene            complement(2802..3698)
                     /locus_tag="VEJ00_00205"
     CDS             complement(2802..3698)
                     /locus_tag="VEJ00_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99604.1"
                     /translation="MDRIAANTLRTLGIVLISAFVIGASLILLLLALCLGAMSNGGGS
                     SSDSQMFLACILIIALLIVLGVFSIARLAKGIVRGEPEYPAPLTIFPKPATTVAVPSP
                     AFPPSPGPPAAAAPRTIDVAAHFSPASRAAIHQVVLAISTKLAVETLIVLIGWTHVYG
                     RPAPFPVYRYSFLAWGLAAAAPLIVLIYALLRHPGPRAFAYSLVIPALHIFFGTFGHS
                     ATIFILFRANPAIPPVISLFALLPWLLDFLILYLAWKAIRLTGIQPSPTRLIVATVVI
                     FFYTSVLPIFVVIMNQFWRLAR"
BASE COUNT          774 a          944 c         1429 g          725 t
ORIGIN      
        1 tccacggcgc ggagggtgac aacgtcgaac gaggtgggcg gcgagtgctt caggagctct
       61 tctgcacgat aggtcactac attgacgttc gtcaatgata gcgcgcgaac agtctcgcga
      121 aggaaggtgg ccttcttgtg gttgggctcg atcagggtga gggaaacgtg gggcgcccag
      181 agcttgatcg ggagaccggg gaagccggcg cctgaaccaa catcggcgac gtggacaccc
      241 ttcgccgcag agttcgcgga gaacgcgggg aacagaatgc gggcggcgaa gaagctctct
      301 ccgaagtggc gagtgacaat ttcttccggg tcgcggatcg cggtaaggtt gatgcgcgcg
      361 ttccagcgca ggattagatc gatgtatatc gatatacctc gaagttgagc cggggacagg
      421 acatcaggct tctcgcgggc cgaggcccgc tcggaacgac aagagggtga gagtttggcc
      481 gaaaggaagg gttcgagtag ggcggcgatg cgggccggat ccatgagggc ctcccgattg
      541 tagattgggg aatggaaaga gtgatcacaa atcgcctccg cgcggttcga ccacgggggc
      601 taaagccccc ttttcagcgg ctgtcctaac gcggcgctga agcgccgctc ttccacgtgg
      661 tggcaagcga acgaggtcgc ctcgctcctc cgaatgccaa cttttttgat atgaggtgtg
      721 tatcgcgttg cgcaaccgcc cactcaagcc aaagtcgggc ttgagtgggc ccacccgatg
      781 agtttttgtt tccccttttt cctcatcttg cgatctcgta cgccggggcc aacgctggat
      841 ccgcgactct tttcagtgga tcgaggcggg tttgctccag gtcatcgcgg tagaagaggt
      901 tgtaagtgtc gaaggggatc aggcggccgt cggggtggac gatgtggacg caggtttttt
      961 tgacgctgcg gacgtcgaag gagtgggcgt cgaggaattg catgatgatg acgcggaaga
     1021 tgttttcgta ggtgagggag cgcacagcag gttcctcacc gccgctgcgc gccggttcgg
     1081 aatgacagga ctgttgcggg ctatttggaa tccaaacagg ggggaggcag cagaggagct
     1141 cgcggagggt gccggcgctg gattgcggcg agtgatttgt agcaaatagc ttaaagagat
     1201 tgtcacgtac ggacggttcc tgctcgtaaa ggatcgtgtt cttggcgccg ttgatcagca
     1261 cttcgggcgg gatcatgctg gtgagaggag tgactcttcc gtttagcttt aaggcatagg
     1321 ccatggcgag tgagtccggg tgacagggca cgggtatgac gtcctcgggg cggaagacct
     1381 gggtttgttc gagaatcttg cggcggactt cggtcagagt caggcggtcg gtggctggat
     1441 tgaagttgtg gacatagcct ccgttgccgt caaacccccc acttctccca acatcgggga
     1501 gaagtggggc acccgagtca gtgagcacat ctgggggccg cacgaatgcg tccgtcccta
     1561 cccggttcgg ggcccaggtc tccaggcggc cggcgtcctg gatgggctgg aaggtgacgc
     1621 ctctcacgca cggctgctgg agggcgtagt cgatggtctt gccgatttcg tgatcgttca
     1681 gtcccttctt cagcgtgacg acgaggttcg tagagatgtt gtagcggttc agcttttcga
     1741 tggcgtcttc gcggatgcgg cggaggtcgg cgccgcggag ttccatcagc gggtcgcgct
     1801 cgaaggagtc gaattgcagg tagacttcga agtcttcttt gatctccgcg aggcgttgga
     1861 cgaagtcttc ctcctgggcg atgcggacgc cgttggtgtt gaccataagg tgcttgatgg
     1921 ggcgggcttt ggcgagttct acgatttcga agaactgagg atgaacggtg ggctcgccgc
     1981 cggagagctg gaccacgtcg ggatggcctt cgttgcggac gacggcgtcg agcatttttt
     2041 caatttgctt gaggtcgcgg aactgctggc gctcggggcc gctcgaggcg taacagacgg
     2101 ggcagcggag gttgcagtag tcgcagattt cgacgagggt gaggcaggaa tgctgctcgt
     2161 ggtcggtgca gaggccgcag tcgtaggggc atccccattt gacgggggtg ttgtagacga
     2221 ggggcatttc ggggggcttg atgaagactt cgcggcagcg gcggtagtag tcgacgtcgt
     2281 cggcgatgag gatgcgctcg gggccgtggt cggggcagcg cttgaggagg tagacgttcg
     2341 tgtcctggaa gacgattttg gcgtcgacgc gacgatagca ggtggagcag atggagatgg
     2401 cgatgtcgta gaagaggtag gggcgggttt tggccatggg acctcggggc gctcttgggg
     2461 caggcttccc tgctcaaggg cagaagagtc ttttaccaca gagagcacgg ggggacacag
     2521 gggccatcgg cgttttcagg cctgctgagg aaggcgacca caggggctga agcccctttt
     2581 tcccggaacc gtaacgcggc gctgaagcgc cgctcttcca cggtgtggtt aggtccggtg
     2641 tgcgaggccg aggggcccgt tcttccacgt cgcatcgaag gcttgccaca aagggtactg
     2701 aaggcagggg aagtcaaaat ccccacccta acgtcgcaca ggacgcgacg ttaggatggg
     2761 gcacccgcgt attccaagcg ccgggcgttt tgcttcgggg ctcagcgagc taggcgccag
     2821 aattgattca tgatgacgac gaagatgggg agcacggagg tatagaagaa gatcacgacc
     2881 gtggcgacga tcaggcgcgt tgggctcggc tggatgccgg tgaggcggat ggctttccag
     2941 gcaagataca gaatgaggaa gtcgaggagc caggggagca gagcgaacag cgaaatcacc
     3001 gggggaatcg cggggttggc gcggaacagg atgaagatgg tggccgagtg gccgaaggtc
     3061 ccaaagaaaa tgtgaagggc tggaatgacc agagagtatg cgaaggcgcg agggccgggg
     3121 tgacgcagca gagcgtagat cagcacgatg agcggcgcgg cggcagcgag gccccaggcc
     3181 aggaagctgt agcggtagac ggggaatgga gcgggacggc cgtagacgtg cgtccatccg
     3241 ataaggacga tgagggtttc gacggcgagt ttggtggaga tggccaggac cacttgatga
     3301 atcgcggcgc ggctggcggg cgagaagtga gcggcgacgt cgatggttct gggcgcggct
     3361 gcagcaggcg gtcccgggga aggcggaaaa gccggagacg gcacagcgac ggtcgtagcc
     3421 ggcttcggga agatggtcag gggagcggga tactcgggct ctccccggac gattcccttg
     3481 gccagtctgg cgatgctaaa cacgccgaga acgataagca gcgcaatgat gaggatgcag
     3541 gcgaggaaca tctgggaatc cgaggaactg cctccgccgt tgctcatcgc gcccaggcag
     3601 agggcgagca ggagaaggat cagcgaggcg ccgatgacaa aggcggaaat gaggacgatg
     3661 ccgagagtgc gaagcgtatt ggcggcgatg cggtccactt tcgagcggaa tcatacaaga
     3721 atcatgtaaa gaagccgagg cggaaatctg cgccggccct caggcgggct tgaacagagt
     3781 ggaatggaga gatgacgacc gcagggggct gaagcccccg ctatctcgcg gcttaacgcg
     3841 gcgctaaagc gccgctcttc cacccgtagc cg
//
LOCUS       DASPUL010000010         2592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_412480, whole genome shotgun sequence.
ACCESSION   DASPUL010000010 DASPUL010000000
VERSION     DASPUL010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412480"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VEJ00_00210"
     CDS             <1..332
                     /locus_tag="VEJ00_00210"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXW99605.1"
                     /translation="LAASVAVGWMLFHYALPISSALLRAGLIARRDGLFALEPPAMGP
                     VILLTILLNVAAQLGDLVESLIKRGAGAKDSGSILPGHGGMLDRIDALLFAAPVLWFY
                     VAWRVMQ"
     gene            600..1097
                     /locus_tag="VEJ00_00215"
     CDS             600..1097
                     /locus_tag="VEJ00_00215"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HXW99606.1"
                     /translation="MSTRLYWLDERWPGKLAISARPRGGDWLQDEIADWKRSGIDVVL
                     SLLERAEEKEMNLENEAEMAQAQGIEFSSLPIPDRQPPNSEAHVTSTLDRMTKALKAG
                     KNVLVHCRQGIGRSGLIATCLLMRKGMSPGAAREKVTAARGVEVPETKEQREWIDHYA
                     PALAK"
     gene            1111..2322
                     /locus_tag="VEJ00_00220"
     CDS             1111..2322
                     /locus_tag="VEJ00_00220"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF009114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXW99607.1"
                     /translation="MKRIAILGSTGSIGRSTLSVAESYPDRFEIVALAAGNNLDVALE
                     QAKRWRPRVVSMAAEKDADTLRSRLKKEGFNKIEVVHGPAGTVRVATHSEVDFVVSAI
                     VGVAGLEATYEAVKAGKTVGLANKECLVAAGELITAEARKQARPLLPIDSEHNAVHQC
                     LRGGRMDEVERIWLTASGGPFLNTPRSEFASITVQQALNHPTWKMGRRITIDSATLMN
                     KGFEVIEACRLFHIPPQKVQVIVHPQSTIHSMVEFVDGSILAQVSVTDMRLPILYALT
                     YPERIASDMRFAVSDLRHLDFCPPDLKKFPCLRLAYEAAEAGGAKTVALNAADEVAVA
                     AFLDGSIRFDEIPRIIEDVLDETNSGKLESIASVLKADAEARRSAQERVAQTSKTRGK
                     DVAHPPVTSFA"
     gene            2363..>2592
                     /locus_tag="VEJ00_00225"
     CDS             2363..>2592
                     /locus_tag="VEJ00_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXW99608.1"
                     /translation="MSDFSNWLYYFYIIPVLGFMILIHEFGHYAVAKWLGVRVEVFSI
                     GFGKRLFGFRRGETDYRIAALPLGGYVKMSGE"
BASE COUNT          551 a          784 c          750 g          507 t
ORIGIN      
        1 cattggcggc cagtgttgcc gtcggatgga tgcttttcca ctatgcgctg ccgatcagct
       61 ccgccctgct ccgcgccggt ctgatcgcgc gccgcgacgg cctattcgcc ttggagccac
      121 cggcgatggg gcccgtgatc ctgctgacga tcttgctcaa cgtcgctgcc caacttggcg
      181 atctggtcga gtctctgatc aagcgcggcg ccggtgcaaa agactcgggc tcgatcctcc
      241 ccggccacgg cggcatgctc gaccgcattg atgccctgct cttcgccgct cccgtgctgt
      301 ggttctacgt agcctggcgc gtgatgcagt gacaagatga ccacggattc gccaaaacct
      361 ccgcctttga agccactcca tcctgatagc tctttgaaag cagataagct caccaggatg
      421 gacaaagcct ctaccgagag cctgatagcg tcccttaagc cggggttgaa ggactgtctg
      481 aaaacccgac cggatggcac tatactggat ggacaccacc gcatttatgt tctgcgccgg
      541 cggggtgtag acgtagatcg gttgccgcga gaagaagtcg tcaaggatga agcgtaagaa
      601 tgtcgaccag actctattgg cttgacgaac gctggccagg aaaactcgcc atatccgctc
      661 gtccgcgggg gggagactgg ctgcaagatg aaattgccga ctggaagcgg tccggcatag
      721 acgttgtgct gtcgcttctg gaaagggcgg aagagaaaga gatgaatctg gagaacgagg
      781 ccgagatggc gcaagcgcaa ggaatagagt tctcgtccct cccgatcccg gatcggcaac
      841 ctccgaactc tgaggcgcac gtaacctcaa cactggatcg aatgacaaag gctttgaagg
      901 ctggaaagaa tgtgctggtg cattgtcgcc aaggcattgg ccgcagcggc ctgattgcca
      961 cgtgcctgct aatgagaaaa ggcatgagcc caggtgccgc gcgggaaaag gtcacggcgg
     1021 caagaggagt tgaggttccc gagacgaaag aacaacgcga atggatcgac cactacgcgc
     1081 cagctctcgc caagtagagc cgctcaattc atgaaacgca tcgccatcct cggttctacc
     1141 ggctccatcg gccgcagcac gctcagcgtc gccgagtcct atccggaccg cttcgagatc
     1201 gtggcgctgg ccgccggtaa caatctcgat gtcgcgttgg agcaggccaa acgttggcgt
     1261 ccccgggtgg tctccatggc agcggaaaag gacgccgaca cccttcgttc acgtctcaag
     1321 aaagaaggct tcaacaaaat cgaagtcgtc cacggtcctg ccggaactgt gcgcgtggcc
     1381 acgcattctg aggtcgactt cgtagtcagc gccatcgtag gcgtggctgg tctcgaagcc
     1441 acctacgagg ccgtcaaagc aggaaaaacc gtcggcctcg ccaacaagga atgcctggtt
     1501 gccgccggcg aactgatcac cgccgaggcc cgcaagcaag cgaggcccct gctgcccatc
     1561 gacagcgagc acaacgccgt ccaccaatgc ctgcgcggag gccgcatgga cgaagttgag
     1621 cgcatctggc tgacggcctc cggtggcccg ttcctgaaca cgccgcgctc ggaatttgcc
     1681 tcaattacgg tgcaacaggc gctgaaccat cccacctgga agatgggccg ccgcatcacc
     1741 atcgactccg cgactctcat gaacaagggc ttcgaagtca tcgaggcgtg cagactgttt
     1801 cacattcctc cccaaaaagt gcaagtcatt gtccatcccc agtcgacaat tcactccatg
     1861 gtcgagttcg tggatggcag catacttgct caggtctccg tgaccgatat gcgcctgccg
     1921 attctctacg ccctgactta tcccgagcgg atcgcctccg atatgcgctt tgcggtcagc
     1981 gacttgcgtc atctggactt ctgccccccg gacctgaaaa aatttccttg tctgcggctc
     2041 gcctacgagg ccgccgaggc cggcggagcg aaaaccgtag ccctgaacgc agccgatgaa
     2101 gtcgcggtgg ccgccttcct cgatggcagc atccgcttcg atgagattcc caggatcatt
     2161 gaagacgttc tcgatgaaac gaattccggc aaactggagt ctattgcaag tgtgcttaaa
     2221 gccgacgccg aagcccgccg aagtgctcag gagcgggttg cgcaaacgag caaaacacgc
     2281 gggaaggatg ttgcacatcc gcccgtaaca tctttcgcct agtttcctga atctaagccg
     2341 tgggagtgaa cgtatctcga ctatgtctga tttctcgaac tggctgtact acttctacat
     2401 catccccgtc ctgggattca tgatcctgat ccacgagttc ggccactatg ccgtggccaa
     2461 gtggctgggg gtgagggtcg aggtgttttc catcggcttc ggcaagcgtc tgttcgggtt
     2521 ccgccgcggc gagaccgact accgcatcgc cgctctccca cttggtggct acgtcaagat
     2581 gagcggcgaa aa
//
LOCUS       DASPUL010000011         5406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1235218, whole genome shotgun sequence.
ACCESSION   DASPUL010000011 DASPUL010000000
VERSION     DASPUL010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235218"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VEJ00_00230"
     CDS             complement(<1..981)
                     /locus_tag="VEJ00_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HXW99609.1"
                     /translation="MTSLAQETQEPLRQVGLSTEVSDRERYSRQILFRGIGEQGQQKL
                     AAARVAIVGCGATGSALAGLLARAGVGTLRIVDRDYVESSNLQRQSLFDESDAAESLP
                     KAIAAARKISAFNSRIVVEPKVDDLVPDNIETLLEGLDLILDGTDNFETRYLINDYAV
                     ARSRSWIYSAALGSYAVTMNVLPGQTACLACVFPDSPRGMVETCETSGILNSAVNLVA
                     SIAATEALKLLVGSVQAPDLRQTLLSFDLWTNEHAEVSTAKPRPGCRACGERDFIHLA
                     GKGRPHITLCGRNSVQIHERHRPIDFEEMDRRLRPHGSVRHNDFVLKFWHE"
     gene            1382..4378
                     /locus_tag="VEJ00_00235"
     CDS             1382..4378
                     /locus_tag="VEJ00_00235"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HXW99610.1"
                     /translation="MQSIQLPGVEERDRGHLLDLLPQKSGKPLDRGQVADSIRVLYGT
                     GRFADIQAEIVPAGDGVVLTFSTSANFFVGAVNVEGAPDHPNANQIVNASKFQLGERY
                     TRNKLDRALENLRQLLQEDGYYRAAVTAESTANAATQQTDILFHVTPGEPAHVGDVTV
                     TGTSGLSSAVVMSIAHMGRGDRVSSAHFNSWLQRLRKKFQKENRVLAQVSVAEQKYLP
                     VTNAVDYTFQIDPGPVVEIRADGYHISRSVLKKEIPVYEENAVDDDLLNEGQRNLLDY
                     LQTRGHFDASVDIEKDTSDPKTFRVTYQIDPGPVHKLVLVEITGNKEFLDTAKLKSYL
                     QIQPATRFLSHGRYSSSLLKNDVATLEQLYRSNGFRQVRIETKVDDDYNGVDNQLAVH
                     IQIDEGLRTRVGEVEIVGNHEVAINDLPERNTQSGQPYSEQNLADDRERILNYYFNNG
                     FPNASLEITTKPSASQPDVEDVTFTIQEGERFTVNRVMVAGTESTRDYVVDREIQVRE
                     GEPLSQQDLLNTQTRLYNLGIFSQVDTAVQNPEGQDPQKNVLVQLQEAKRYTFTYGAG
                     LEFQTGQPAGTTAPAGTTGVSPRVGFDVTRLNFGGRDQTITFQSHVGRLQQRGLVSYT
                     VPKLLDSDKWKVIYTVFYDNSLDVATFTSQRLEGKIDLRQQIGNSGNEPGTRAGPSSL
                     TYRLEFRRVKASNFAEGINPGEIALFSLPATVGGPGLTFIRDKRDNPLESTKGNYFTL
                     DVFTASKYFRSEADFGRVLAQNSTYQAFGGKGKAEHQFVFARSTTIGLQQPFRNTRVV
                     APGACPTNPITNESVCEGFSLIPLPEQFFAGGGNSHRGFGLNQAGPRDPGSGFPLGGT
                     AVFVNSLELRFPPLTLPYLGEGFGLAVFHDMGNVFTAPHDMLNGLLRWHQSNPQQCLS
                     GGTTNSQCMTQFDNSGYDYTSHAAGIGVRYKTPIGPLRFDFGYNLNPTRYFQLVPIPN
                     TKPTQSVQEALRLRHFNVFFSIGQPF"
     gene            4378..5079
                     /locus_tag="VEJ00_00240"
     CDS             4378..5079
                     /locus_tag="VEJ00_00240"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXW99611.1"
                     /translation="MKRTRAKTTAAVPALALLLSLFVGLPEARADVIDRILATVNGHV
                     ILQSDLDEALCYEAVLNGRQLDQFSGEDRRAVLDQLIDQELLGEQMKSSFFQHASESE
                     AAARIADARKQYPDAATDEGWQALLARFQLTEKDVVAHVQQEIDLMRLVDAHLRPTIQ
                     IDSKTIETYYREKFVPQLKQSGADDVPLADVSAKIRELLTQQRVNELLVSWLQTLRSE
                     SRVSVPGTTQGVETQ"
     gene            5076..>5406
                     /locus_tag="VEJ00_00245"
     CDS             5076..>5406
                     /locus_tag="VEJ00_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99612.1"
                     /translation="MTEPAPRPRRRWKFWLIAMGAVGVALLAGLWYTTTDSFQAYARA
                     KIVEELERITGGRAEIGSFHVVPFHTQIEVRDITVHGSESPSDVPLAHADHLVAKMKV
                     TSFLRAEF"
BASE COUNT         1154 a         1645 c         1588 g         1019 t
ORIGIN      
        1 ctcatgccag aacttgagca cgaaatcgtt gtggcgcacg ctgccgtgcg ggcgcaatcg
       61 ccggtccatt tcctcgaaat cgattggccg gtggcgctcg tggatttgca ccgagttcct
      121 gccgcacagc gtgatatggg gccgcccctt gcccgccaaa tgaatgaagt cgcgctcccc
      181 gcaagcccgg catcccggac gcggcttggc cgtggagact tccgcgtgct cgttggtcca
      241 gagatcgaac gagagcagag tctggcgcaa gtcgggcgct tgaacgctgc cgaccagcag
      301 cttgagcgct tctgtagccg cgattgacgc caccaggttg acggccgaat tcagaatccc
      361 ggaagtctca caggtttcga ccataccgcg tggagagtcg gggaaaacgc aggccaggca
      421 ggcggtttgg ccaggcaata cattcatggt gacggcatag cttcccagcg cggctgaata
      481 gatccacgag cgcgaacggg caactgcata gtcgttgatc aggtagcgag tctcgaagtt
      541 gtcggtgccg tccaggatga ggtcaagtcc ttccagcaga gtttcaatgt tatccggaac
      601 cagatcgtcg accttcggct ctactacgat ccgggaattg aaggcggaga tcttgcgagc
      661 tgccgcgatc gctttgggca gggactcagc cgcatcgctt tcatcaaata gagactggcg
      721 ctgcaggttg ctggactcga catagtcgcg gtcgacgata cgaagcgttc ccacgcccgc
      781 gcgggccaac aaccccgcca gcgctgaccc ggttgcgcca cagccaacga tcgccacgcg
      841 agcagcggcc agcttttgct gaccttgctc tccgataccg cgaaacagaa tctggcgaga
      901 atagcgctcg cggtcggaaa cctcagtcga aagcccgact tgccgaagtg gttcttgtgt
      961 ttcctgtgcc aaagaggtca ccagtgggct gccgcaaaac ctttattata agtggctggc
     1021 tttgcaatgc tgctcgcggg ttgaatatca atggacggca acccgcacca tggcaatacg
     1081 cggcggaaga actcgtacaa caactgccgg gcaggctcca tcgaacgcaa taacgcccgg
     1141 actcaaggac acctttttgt cgagacggca cgcgacatcc ctgaccgcga tgatcctctt
     1201 gtggattgcg gttcagccgt ccgccgcggc ttcgcgggat ttcagcgcgg ggagtcaaga
     1261 tgcgggctcc agtcagcagg ggccctcaaa accagcttcc gaaagctccg cccaggcgcc
     1321 agtgcctcgg caagagcctt cccattccgc ttcaggattc tctggctacg aaggaaagct
     1381 ggtgcaatcg atccagctgc ccggcgtcga agagcgcgac cgcgggcacc tgcttgatct
     1441 gcttccccag aaatcgggga agccgctcga ccgcggccag gttgctgaca gcattcgcgt
     1501 tctgtacggg accggccgtt ttgccgacat ccaggcggag atcgttcctg ccggcgacgg
     1561 tgttgtgctg accttcagta cgtcggctaa cttctttgtc ggcgcagtaa acgtggaggg
     1621 agctcccgac catcccaatg cgaaccagat tgtgaatgct tcgaaatttc aattgggcga
     1681 acgctacacg cgcaacaagc tggatcgggc gctcgaaaac ctccgccagc tcctgcagga
     1741 ggatggctac taccgcgcgg cagtgacggc tgagagcacc gccaacgcag ccacgcagca
     1801 gaccgatatt ctttttcacg tcactccggg ggagcccgcg cacgtgggcg acgtgacggt
     1861 gaccgggacc tcgggacttt caagtgctgt ggtcatgagc atagcgcaca tgggacgggg
     1921 tgatcgtgtc agttccgcac acttcaacag ctggctgcag cgtttgcgta agaagtttca
     1981 gaaagaaaat cgcgtgctgg cgcaggtatc ggtagcagaa cagaaatacc ttccggtaac
     2041 caacgccgtg gactacacct tccagatcga cccgggtcca gtcgtcgaga tccgtgccga
     2101 tggttaccac atcagccgca gcgtgctgaa gaaggagatt ccggtctacg aagagaacgc
     2161 ggtcgatgac gacctgctca acgaaggcca gcgcaatctg ctcgactact tgcagacgcg
     2221 aggccacttc gacgcttccg tagacatcga gaaggatacc agcgatccca agacttttag
     2281 agtgacatac cagatcgacc ccggacccgt gcacaagctc gtactggtag agatcacggg
     2341 caacaaggag ttccttgaca cggccaagct gaagtcctac ctgcagatcc agccggccac
     2401 acgctttttg agccacgggc gctacagcag ttcgctactc aagaatgacg tcgcgacgct
     2461 cgagcagctg tatcgctcca acgggttccg ccaggttcgc attgagacca aagtcgacga
     2521 cgactacaac ggcgtagaca accaactggc cgtgcatatt cagattgatg aagggctgcg
     2581 cacccgggtc ggcgaagtag agattgtggg caaccacgag gtggcgatca acgatcttcc
     2641 cgagcgcaac acgcagtcgg ggcagcccta ttccgagcag aatctggcgg atgaccggga
     2701 gagaatcctg aactactact tcaataacgg ctttcccaac gccagcctcg agatcaccac
     2761 caagccgtcc gcaagccagc ctgacgtcga agacgttacc ttcaccattc aggaaggcga
     2821 gcgcttcacc gtaaaccgag taatggtcgc cgggaccgag agcacccgcg actacgtggt
     2881 ggatcgcgag atccaggtgc gcgagggaga gccgctcagc cagcaggatc tcctgaacac
     2941 tcagacccgt ctctacaatc tcggaatctt cagccaggtc gacactgcgg tgcagaatcc
     3001 ggaaggccag gatccgcaaa agaacgtttt ggtgcaactg caggaggcca agcgctacac
     3061 cttcacctat ggagccggac tcgaattcca aaccggccag cccgccggaa ccaccgctcc
     3121 ggcaggaacg accggggtca gccccagggt gggcttcgac gtgacccgcc tgaactttgg
     3181 cgggcgcgac cagaccatca cctttcagtc tcacgtaggc cgcctgcagc agcgggggct
     3241 ggtcagctac accgtcccga aactgctcga cagcgacaag tggaaagtca tctacaccgt
     3301 cttctatgac aacagcctcg atgtggccac gttcacctcg cagcggctgg aaggcaagat
     3361 cgatctgcgg cagcagatcg gaaactccgg caatgaaccg ggaacccggg cggggccgag
     3421 ttcgctcacg tatcgcctgg agttcagaag ggtgaaggcc agcaacttcg cggaagggat
     3481 caacccgggc gaaattgcgc tgttttcgct tcccgccacg gtgggcggtc cggggctgac
     3541 ctttattcgc gacaagcggg acaatccgct ggagagcacc aagggaaact acttcacgct
     3601 cgatgtcttc accgcttcca agtatttccg ctcggaagcc gacttcgggc gcgtgctggc
     3661 gcagaattcg acctatcagg cgtttggcgg caaggggaaa gccgaacatc agttcgtatt
     3721 tgccaggtcc accaccatcg gcctgcagca acccttccgc aatacccggg tggtagcgcc
     3781 gggagcctgc ccgaccaatc cgattaccaa cgagagcgtg tgcgagggct tctccctgat
     3841 tcccctgccg gaacagtttt ttgccggcgg cggcaactcc catcgcgggt tcgggttaaa
     3901 ccaggcaggc ccccgtgacc ccggctctgg ctttcccctg ggcggtaccg cggttttcgt
     3961 caacagcctg gaactgcgct ttcctcctct gacgctgccg tacctgggcg agggtttcgg
     4021 cttggccgtt tttcacgata tgggaaacgt tttcacagct ccgcacgaca tgctgaacgg
     4081 gctcttgcgc tggcatcaga gcaaccccca acagtgcttg tccggaggta caacgaactc
     4141 acagtgcatg acccagtttg ataactccgg ttatgattac acttcgcacg ctgctggaat
     4201 tggcgtacga tacaagacac cgattgggcc gctgcgtttt gactttgggt ataacctgaa
     4261 tccgaccagg tatttccagc tggtcccgat tccaaacacg aaaccgaccc aaagcgtgca
     4321 ggaggcgttg cgccttcgcc atttcaacgt ctttttcagc ataggccagc cattctgatg
     4381 aagagaaccc gcgcgaaaac gactgccgct gtgccggcgc tggcacttct gctgtctctg
     4441 ttcgtcggcc tgccggaggc acgggctgac gtgatcgacc gcatcctggc caccgtcaac
     4501 ggccacgtca ttctgcaaag cgatctggac gaggccctgt gctatgaggc cgtgctgaac
     4561 ggccgccaac tggaccaatt cagtggcgaa gaccggcggg cggtgctgga tcaattgatc
     4621 gatcaggaac ttttgggcga gcagatgaag tcctcctttt tccagcacgc ctccgagtcc
     4681 gaggctgccg cgcggatcgc cgacgcgcgc aaacagtatc ccgatgctgc gacggacgag
     4741 ggctggcagg ctttgttggc cagattccag ttgacggaaa aagacgtggt ggcccacgtg
     4801 cagcaggaaa tcgacctgat gcggctggtc gatgctcacc tgcggcccac gattcagatc
     4861 gactccaaga ccatcgaaac atactaccgc gagaagtttg tcccccagct caaacagtcc
     4921 ggtgcggacg atgttcctct ggctgacgtc tcggccaaga tccgcgaatt gctgacgcag
     4981 cagagagtca acgaactgct ggtctcctgg ctgcagaccc tgcgctcgga gagcagggtg
     5041 agcgttcccg gcacaaccca gggagtagag acgcagtgac cgagcctgcc ccccggcctc
     5101 ggcgcaggtg gaagttttgg ctgatcgcga tgggggcggt cggcgttgct ctgctggcag
     5161 gactctggta taccaccaca gattcctttc aagcctacgc ccgggcgaag attgtcgaag
     5221 agttggagcg aatcaccggt ggccgcgctg aaatcggcag cttccacgtc gttcctttcc
     5281 acacccagat cgaagtgcgc gacatcacag tgcatggcag cgagtctccg tccgacgttc
     5341 cgttggccca cgccgatcat ctcgtggcaa agatgaaggt gacctcgttt ctgcgcgccg
     5401 agttcg
//
LOCUS       DASPUL010000012        19069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1646373, whole genome shotgun sequence.
ACCESSION   DASPUL010000012 DASPUL010000000
VERSION     DASPUL010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 19069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19069)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19069
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1646373"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VEJ00_00250"
     CDS             complement(<1..689)
                     /locus_tag="VEJ00_00250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXW99613.1"
                     /translation="MTIALSLPGKVALITGGSRGIGAATVRSFCAAGAKVMFNYQRAK
                     SAAEALVKQCGAERCAAVACDLAGLSTAKDLVEETVKRFGRIDILVANHGIWPPEDMP
                     IDQMTDAQWSGTVAVNLDAVFALIRQSVAHMKRQAKSGGAAGHIVLVSSTAGQRGEAF
                     HADYAATKGALISLVKGLSTELAGHGIYVNCVAPGWVATDMSEPALNDPATRQAIFAT
                     IPLGRVGTPEE"
     gene            complement(854..4240)
                     /locus_tag="VEJ00_00255"
     CDS             complement(854..4240)
                     /locus_tag="VEJ00_00255"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXW99614.1"
                     /translation="MNSIATSVVIIPGVVALLLFLVFTYLYEQSRQAYFRAWQLGWAA
                     YSLHFAMDAWNSYHPPSALVYLAGSMLVVVMALCIFVSTRLMRERFRLRWYDLALGMA
                     GVGLALWNLSAWKAGGPYRVEAAQPHNRLEIGVGLILLYCSFHFYRYAHRRNSVAFRM
                     LALSLALWAALMALGQLRGPFVDLFGNAGHMLGPLPQMLLGIAMVMVLFENERNAVQE
                     NALAFSTLGVDPRRLLTAEDLVPSMTSVLDRLVSPLPTERAVICIAPSWRSVLPSVQR
                     GFSAEFLESLETSGAGEYSCELAYRRGGFFSFRDVPQMTEPLPAFPGARFQDFQQVLA
                     EANIRHLTAVSLQTREHNFGVILFPQAERRLFGASNLRLLIGLALQIGLTLENYVVMH
                     DAQRRTKEYELLTEIGQAISSRLDQEEVLRTIQKELGQIFDTRDFYIAFQEGDEIRFE
                     LEVEKNKVEPKRSRRAINGLTEYIIRTGQPLLIRADLEKERARLGLERAGAGKPAKCF
                     CAAPILLGGKPAGVMAAMSTEREYVFEQRDLEVMKTAAGQVSVAVENARLFAEEQRRS
                     RQLSFLNNISKTAISSEDSEQMLADIVAEIQKNFRFDHIGIGILDYVTKEIEIRAEAG
                     TTAHGKGKRVALGVGILGRVARTGERAVVPSAAEGQLLPESRSVLCIPIAYGESLLGV
                     LNVESQEEKAFSPQDLLIMNTLADLLATALHNAFVFQKLQQQSITDGLTGVKTRRFFW
                     ESLSSEWKRASRSGRPFSVVLVDLDKFKEVNDTLGHLEGDLVLARVGRLLEQRCRQSN
                     VVARYGGDEFIILMPETGMEQAQTLAERLRQWLAQDPTLSEHHITGSFGVASFPMHGF
                     SVEDIIRVADACMYESKHAGGNRVSTAEEIVDGETFATQRQKISGYIEGFLQRERTGP
                     EHLAELVNTLKKLCGSDADCNVQLLRESIEALTRAAESREYHSSGHGDMVARHTEIIA
                     RALHLPPDELAELVYAARVHDVGKIFVPERILARTGPLTEDEYERIKMHAQAGAAIIS
                     TIPGCQMLQVAVAHHHEAFDGSGYPNGLRGEQIPLWARIVGLADAYVNLTTDRSFAPA
                     MSSDQALGELEKMSGTRFDGMLVRILIRELKAERAPRLGD"
     gene            4597..6081
                     /gene="gltX"
                     /locus_tag="VEJ00_00260"
     CDS             4597..6081
                     /gene="gltX"
                     /locus_tag="VEJ00_00260"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXW99615.1"
                     /translation="MRFAPSPTGFLHVGSARTFIFNWLYARRNNGTMILRLDDTDVER
                     NSDASVASIFDGLNWLDLGWDEEYKQSERLALHQKTAQTIFERGLAYRDFTPTHGPEA
                     EKSGTQGTWLFNPGMRELSRSESDRRAAAGEPFALRFRVPRGTGEEVRFLDAVYGDQR
                     KQADAVEDFALLRSDGMPTYHLASCADDADLSITHVIRGQDHLTNTFKHILIFQATGF
                     TPPRFAHLPLLVAPDGTKLSKRRHGPVVSVTTYRDAGFLPEAFINFLCLLGWSPKDNR
                     ERMSREELIDAFSLEGINRANAVVNFTETTAALEAKATAGLEKTVYPEETFDPKAVWL
                     NAEYIRALSIEDLSARLLSVVEGAGYRVDPARMLRITPLIRERIKFLCDVLTAADFFF
                     VDQLPPYDPAELIPQKGDAAMAFKVLEKARAVLAATDFTHDALDHALRSAAQELGLKA
                     GQMFQPIRVAVCGRKNAPPLFETLAVLGSDTTLARIDQAMKKLQ"
     gene            complement(6367..7791)
                     /locus_tag="VEJ00_00265"
     CDS             complement(6367..7791)
                     /locus_tag="VEJ00_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXW99616.1"
                     /translation="MACIRAHMDFLASDALRGRGSGTHDELVAATYVASALEQYDIAP
                     AGDNGSYIQRAVTVRPVLTAPPTMHFMTPGDGIPAQTIVWTHGKEMLVEQISDTEFRG
                     RLKRIILGPEPALREEGRALGAAQEPEAREHEGQQLAPGSGQEPSKDAVVLINGGGAE
                     RIRDTAIGALAAGALAVLLPASAETLRDWHEKGSELPRLPARLQGESETGLGSSFNVF
                     ALSQQALIDLRGIPDGTYIYLETKTGAPEMGSTWNAVGKITGGDPELRKSSILLSAHL
                     DHLGVGEPVKGDSIYNGADDDASGTTAVLELARVLGSGPKPRHTVIFALFGSEEAGGL
                     GSLWFLAHSPVPLAEIAANLEFEMIGRRDPKYPGDTLWLTGWDRTNLGPALAAHGARL
                     AADARPDQHFFMRSDNYLLAKKGVVAQTISSFGLHADYHKPSDDLAHIDFTHMTAAIR
                     SLIPPVEWLVNAAFQPEWRPGGKP"
     gene            8141..8824
                     /locus_tag="VEJ00_00270"
     CDS             8141..8824
                     /locus_tag="VEJ00_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99617.1"
                     /translation="MVTWKLQLVLSFTALFLLALALGCNGFFVDPVLVSIVVAPPTPT
                     LVVGGSGQQMTAFGTFDDGSQKNINSSVAWTMNPPGYATITAGGLLTGTSPSPSTVTL
                     QASDQAVVGSTTFQVFSNITSITVSPATQSVSVSSGTPFCLQAIASTSAGSQDISATA
                     TWTFTDPNNQTVGGITKTTGTCTGQAFQIGTLTPSETLPVTLSVTASAPASGGGTVTS
                     NSVKVGLTQ"
     gene            9585..10586
                     /locus_tag="VEJ00_00275"
     CDS             9585..10586
                     /locus_tag="VEJ00_00275"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXW99618.1"
                     /translation="MRLLSALMLVGLMGAAGCGRGKGRSREAMYVSANQASLRDRVAA
                     VYSRTGEVKNGDRVEVLDRERRFVKVRTTTGAEGWIEQRYLVGQQVYDGFQKLALAHQ
                     SDTVQAPGITRNETNLHIEPGRDSEHLYQLPQGTKISLLERGTAERPNVAAPASATPR
                     KDTKPTGPLLEDWWLARDAEGHVGWLLARMLDVDVPLEIAQYAEGQRFVAFFTLDQVK
                     DGDKQVPQYLAVLTEAKDGLPYDFDQVRVFTWNLKKHRYETAYREHGLNGVLPVTLTQ
                     ESFDREGDLPVFLLRVRDDAGTVVERKYKLNTPIVRRVLAPGEVATPSAGKKKKKRR"
     gene            complement(10653..11030)
                     /locus_tag="VEJ00_00280"
     CDS             complement(10653..11030)
                     /locus_tag="VEJ00_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99619.1"
                     /translation="MNVYFAVAGALLILLALVHSFLGERALLQRLFRRTGLPKLFGDE
                     FYTRRILRFTWHLTSIVWIGAGTAFLLIAVRKPDRTANAVADIFAVVFLLSGMFSVVE
                     VHGRHPSWAVFLLVALLLWIGAR"
     gene            11395..13059
                     /locus_tag="VEJ00_00285"
     CDS             11395..13059
                     /locus_tag="VEJ00_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99620.1"
                     /translation="MRLLSVPLIFPLVLCSGSFAAQSSSTPTSDMSKSKKSKTEAAPA
                     PQTTAPNPPAPAAPEARPESAKPAAGGKEGEEHYDMTEVAPVVTHHQVTLDGKVLKYT
                     ATAGRLPIKRDDGNIEAEMFFVAYTLDGQEAAKRPLTFAFNGGPGSATVWLHMGALGP
                     KHVVLQPNGFMPAAPYRMEDNPNTLLDRTDLVFVDAIDTGFSRAANAEMARKFLGVKG
                     DVEAFGEFVRLYLTRHERWPSPLFLFGESYGTTRAAGLSGYLAERGIAFNGIVLLSMA
                     VDFQTLEWAKNNDEPYILLVPSFASIAAYHKRLAPDLIEDMKRTREEARRWAESDYAL
                     ALAKGDGLTPEERQKIIDQYARYTGLSKQLIDDANLRVDVGQFTHNLLLDQKLRVGRL
                     DGRFTGPDPEGLLDTRFYDPTASAILPPYTAVFNHYVRSELGYQADTPYKVFAWNEEA
                     FHKWEWGDAEKGFPNTAPSLRAAMVKNPYLKILVMEGHYDLATPFAAADYTMDHLNLD
                     PAYRKNISYATYDAGHMVYIDSVSHVKMKKDAVEFIERCLETAASR"
     gene            complement(13089..14333)
                     /locus_tag="VEJ00_00290"
     CDS             complement(13089..14333)
                     /locus_tag="VEJ00_00290"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXW99621.1"
                     /translation="MQLQAWKLDAEACDLPAKIGIDVTQPWTSKTARRLDAVALLDLS
                     RQYAAIREEILAAVERVCASGQFILGEEAAALEHEFSVYTGAAGSVGCASGTEALWLA
                     LAAAGVRPGSSVITTAFSFFASASAIVRAGARPLFVDVDPRTLNLDPAQVEAQLRAGK
                     PNNLRALLPVHLYGQCADMDALNRLSDAFHVPLIEDAAQAVGAAWRGRRAGSLGLAAA
                     FSFYPTKNLSAMGDAGLVTTNSPELAERMRSLRNHGSRRRYLHQDMGWNSRLDAIQAA
                     ILRVKLPHLEDWNRCRRERAANYDRLFTQAGLLSPGDDAPVRILSVAPHAHHVFHQYV
                     IRARRRDALRGFLNERKIGAEIYYPIPLHLQPVFAWLGYREGDLPESERAAREVLALP
                     IFPELTDEEQQWVVESVAEFYS"
     gene            complement(14482..14865)
                     /locus_tag="VEJ00_00295"
     CDS             complement(14482..14865)
                     /locus_tag="VEJ00_00295"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HXW99622.1"
                     /translation="MFNDCESFWPHYEHGIALFNRADFFAAHEELEQVWRAAPPAERR
                     FLQGLIQVAVALHHHSTGNLAGCRSVLGRACRNLAAYPSKHGGLDLAALLAALAHWRL
                     ALDENRTPPPPPVLSLHKQEGSAPG"
     gene            15125..18592
                     /locus_tag="VEJ00_00300"
     CDS             15125..18592
                     /locus_tag="VEJ00_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXW99623.1"
                     /translation="MIQANTSSKLLVLAVAVLAALSLVPGAFGQAISGSLTGSVTDPT
                     GAAVNAATVEVTNIATGFKASTKTQAYGSYLFTELPVGTYTLTVTAANFKTSTLSGIP
                     VDLNKSNTANVKLELGSSTTTIEVSGVAPPVDTTTAQLQSNFDSRFAQDLPITSNGQG
                     AGVLNLSMLAPGVSNANAMGLGVGPSVGGQRPRDNNFTVEGVDNNNKTVTGSLITIPN
                     DAVDEFSLISNQFNSEFGHSSGGQFNTTVKSGTNSFHGSAYEYFRNRNLNAVDQTYVN
                     QGLTSNPRFDSNRFGGTFGGPIIKDKLFFFSNFEYQPTGLTAASSGGASTPTAAGLAA
                     IGADPNLNATNFGIFKQYVPVAATGSACATFNGTSNGVPFGAGFKAPANGICPAGSVE
                     LGAVTITPPAYENYTNFVQSVDFNLSAKDQIRGRYVLNNQTGPDIAAQLPVFFVSLPI
                     KYRLFTVSEYHTFSPTLLNEFRFGFNRFDQTYAVGPQKYPGLDAFPNIVLADLGSAGL
                     NIGPDPNAPQYTIQNFYGFVDNASWTVGKHTLKFGAEYRWYISPQNFTQRSRGDYEYN
                     STQLYLEDFTPDIIGQRSTGSTTYYGNQNAVYWYVNDSWKLKPNLTLNLGVRYEWTSI
                     PLGEQRQSLNEVSNSPNVIIPQVNQPLIFSAPHAPRDQFAPRVGIAYSPGSKGNMSIR
                     AGFGMAYDTLYDNIGILAVPPQVGSTNNVNTNLPPAADFLANGGLKGGGSGITVLDRA
                     DAMASTSSWIPPNVKYPYSINWNLGVQRSFGKNYTADVNYVGTRGNHLDVQNILNFQS
                     VITPQTALPTFFSMPTQAALNALTNSNLNALEQLNIIPDNLNNVDGPGIGFNQSVLTG
                     FIPSGWSTYNGLQAHVTRRFSNGLTLDGAYTWSHTIDNSTADFHSTDLTPRRQQDFFA
                     FTPEKATSALSRTQRLTVAAVYNLPFFKNGNWFMKNIVGNWVFSPIYTYESPEFATVQ
                     AARDANLNGDSAGDRGIVNPVGFKGQGGNVMPLSAVSGPNAGQVVAYVATQDPAGTVP
                     CSSAAQCPEFIRTGLGSISNMGRNTLATYHTNNFDLGTYKDLHLTERMYFRLGAQFGN
                     LFNHPQYIPGVNPGFGLGVNDVYSYSSCCTTSTYRNFATPDKANFNDPKLTFASSART
                     MGLVVKFIF"
BASE COUNT         3710 a         6129 c         5652 g         3578 t
ORIGIN      
        1 atctcctcgg gagtgcccac ccggcctagc ggaatcgtgg caaagatcgc ctgccgggtt
       61 gcgggatcgt tcagcgcagg ttcggacatg tccgtcgcca cccatcccgg agcaacgcag
      121 ttcacataga tgccatggcc agccagttcg gtcgagagac ccttcaccag gctgatgagc
      181 gctcctttgg tggcggcata gtcggcatga aaggcctccc cacgctgccc ggccgtcgaa
      241 ctgacaagca cgatgtggcc ggccgcccca cccgatttgg cctgcctctt catgtgcgcc
      301 accgactgcc ggattagcgc gaacaccgca tccaggttca ccgcgaccgt gccactccac
      361 tgtgcgtccg tcatctggtc aatgggcatg tcctcgggcg gccagatgcc gtgattggct
      421 accagaatgt cgattcttcc gaagcgcttg acggtctcct ccaccaggtc cttggccgta
      481 ctgagcccgg ccagatcgca ggccacggcg gcgcaccgtt cggcaccgca ttgcttcacc
      541 agtgcctctg ccgccgattt cgcgcgctga tagttgaaca tcaccttggc cccggcggcg
      601 caaaacgagc gcacggtcgc ggctccaata ccgcgtgagc ctccagtaat cagggcaact
      661 ttgccgggaa gcgagagagc gatggtcatg ctcctagtct ttcagttccg gtttctctct
      721 ggcaagcgag caggtgttcg gccgggcctc cgcatttctt ccatttcgtc cgacggagaa
      781 cccggggagg gaagcgacgg tgacccccgc cggcgcgggc tttttcgcgg ggcgagggcg
      841 ccccgcgcca ccttcagtct cccagtcgcg gggctctctc cgccttgagt tcgcggatca
      901 ggatgcgcac cagcatgccg tcgaagcggg tgccgctcat cttctccagt tcgcccaggg
      961 cctggtcgct gctcatcgcg ggcgcgaagg atcggtctgt ggtgaggttg acgtaggcgt
     1021 cggccagccc cacgatgcga gcccacagcg gaatctgttc gccgcgcagg ccgttagggt
     1081 agccactccc gtcgaaggcc tcatgatgat gcgctacggc gacctgcagc atctggcacc
     1141 cgggaatcgt gctgatgatg gcggctcccg cctgggcatg catcttgatt cgctcatact
     1201 cgtcctcggt gagcggcccg gtccgggcca ggatgcgctc ggggacgaaa atcttgccca
     1261 cgtcgtgcac ccgtgcggcg taaaccaatt ccgccaattc gtccggcggc aggtgcaggg
     1321 cgcgggcgat gatttccgtg tggcgtgcca ccatgtcgcc atgcccggag gagtggtatt
     1381 ctcgcgactc agccgcccga gtcagcgcct cgatggattc acggagcagt tgcacattgc
     1441 agtcggcatc gctgccgcac agtttcttca gcgtgtttac cagctcagcc aggtgctcgg
     1501 ggccggtgcg ctcccgctgc aggaaaccct cgatgtatcc ggaaattttc tggcgctgcg
     1561 tggcgaaggt ttcaccgtcg acgatttctt ctgctgtcga cacccggttc ccgccggcgt
     1621 gtttcgattc atacatgcac gcgtccgcca cccggataat gtcttcgacc gagaacccgt
     1681 gcatgggaaa gctggccact ccgaagctcc ccgtgatgtg atgttcactc aaggtcgggt
     1741 cctgtgccag ccattgccgc agccgttcgg ccagcgtctg cgcctgctcc atgccggtct
     1801 ccggcatcag gatgatgaac tcatcacctc cgtagcgcgc caccacgttg gattgccggc
     1861 aacgttgctc cagcaatctc cccacccgcg ccagcaccag gtcaccttcc aggtgcccga
     1921 gcgtgtcatt cacctccttg aacttgtcga gatccaccag cacgaccgag aaaggccgcc
     1981 ccgagcgcga cgcccgcttc cactccgacg acagcgactc ccagaagaag cgtctcgtct
     2041 tgaccccggt caggccgtcg gtaatcgact gctgctgcaa cttctggaac acgaaggcgt
     2101 tgtgcagcgc cgtggccagc aggtcggcca gggtgttcat gatcagcaga tcctggggcg
     2161 agaacgcctt ctcctcctgg ctctcgacgt tcagcactcc cagcagcgac tcaccgtagg
     2221 caatcgggat gcatagtacc gagcgcgatt ccggcaacag ctgcccttcg gcggcactcg
     2281 gcaccactgc ccgctcgccg gtgcgcgcca cccggcccag gatccccacg cccagggcca
     2341 cccgcttgcc cttgccgtgc gcggtggttc ccgcctcggc acgaatctcg atctccttgg
     2401 tcacgtaatc cagaatcccg atgccgatgt ggtcgaaacg gaagtttttc tggatttcgg
     2461 ccacgatgtc ggccagcatc tgctcagagt cctcgctgga gatcgccgtc ttggagatgt
     2521 tgttcaggaa actcagctgc cgtgaccgcc gctgctcctc ggcaaacaag cgggcattct
     2581 ccaccgccac ggagacctgt cccgcagccg tcttcatcac ctccaggtcg cgctgctcga
     2641 acacgtactc ccgctcggta ctcatggccg ccatcacgcc ggcaggtttg cccccgagca
     2701 agatgggcgc tgcgcagaag cacttggccg gtttgcccgc tcctgccctc tccaggccca
     2761 ggcgcgcgcg ctctttttcc aggtctgcgc ggatcagcag cggctgcccg gtacggatga
     2821 tgtactcggt caacccgttg atggcccggc ggctgcgctt gggctccacc ttgttcttct
     2881 ccacctcgag ctcaaagcgg atttcgtcgc cttcctgaaa ggcgatgtag aaatcccgcg
     2941 tgtcgaagat ctgccccagc tccttctgga tggtacgcag tacctcctcc tggtcgagcc
     3001 gcgagctgat cgcctgcccg atttcggtca gcagttcgta ctccttggtc cgccgttgcg
     3061 cgtcgtgcat gaccacgtag ttctccagcg tcaacccgat ctgcagcgcc agcccgatca
     3121 gcagccgcag gttggacgcc ccgaacagcc gccgctcggc ctggggaaac aggatgaccc
     3181 cgaaattgtg ctcacgcgtc tgcaggctca ccgccgtaag gtgacggatg ttggcctccg
     3241 ccagcacctg ctggaaatcc tggaagcgcg cccccggaaa cgccggcaag ggctccgtca
     3301 tctgcggcac atcgcggaag gagaagaatc cgccccggcg gtaggccagt tcgcagctgt
     3361 actccccggc gccgctcgtt tccaggcttt ccaggaactc cgcggaaaat cctcgctgca
     3421 ccgaaggcaa cacgctgcgc cagctcgggg caatgcagat aacggcgcgc tcggtgggca
     3481 gggggctgac caggcggtcg agcaccgagg tcatgctcgg caccaggtcc tccgccgtga
     3541 gcagccggcg cgggtctacc cccagggtgg aaaatgccag cgcgttctcc tgcaccgcgt
     3601 tgcgctcgtt ctcgaacagc accatcacca ttgcgatgcc cagcagcatc tggggcagag
     3661 gccccagcat atggcccgca ttcccgaaga ggtcgacgaa cggcccacgt agctgtccca
     3721 aggccatcag cgccgcccac agcgccagcg aaagcgcgag catccggaac gccaccgaat
     3781 tacgccggtg cgcgtagcga tagaaatgga acgagcagta caagaggatg agacctaccc
     3841 cgatctcgag ccggttatgg ggctgcgccg cttccacccg gtagggcccg cccgccttcc
     3901 aggccgacag gttccaaagc gccagcccga ccccggccat gcccagcgcc aggtcgtacc
     3961 agcgcaagcg gaaacgctcc cgcatcagcc gggtcgaaac aaaaatgcac aaggccatca
     4021 ccacgaccag catcgatccc gcgaggtaca ccagcgccga gggcgggtgg taggagttcc
     4081 aagcgtccat ggcaaagtgc agcgagtacg ccgcccagcc caactgccag gcgcgaaagt
     4141 atgcctgccg gctttgctcg tacagataag tgaagacgag aaacagcagg agggcgacca
     4201 ccccgggaat gatgaccacg ctggtggcga tgctgttcat ggcgccagaa tcagcactcg
     4261 atctttccgc aagaccacga tcctcactga gagttgcagt ggcacaatcg tttcacagcc
     4321 gcaaccccaa aacaatacgt ttgaagtaca ggggtgtggg aaactcgccg gcaaccgcgc
     4381 gaatctctga cagcgctttt tcccagcgcc agtccggcag cctgccgaag tggaacggtt
     4441 ctcccaatgc cggatttctc ggtcggtcga acccaggcgg tctggttccg ccacgcttga
     4501 cgctcgattt ctcctgcccg taccatccag acggaccatc tccccatgct tgcctcttct
     4561 actcctttca aagcacgcga cttatccggt ccccgcgtgc gttttgctcc ctccccaacc
     4621 ggcttcctcc acgtgggcag cgcccgcacc tttattttca actggctcta cgcccgccga
     4681 aacaacggca ccatgatcct gcgcctcgac gacaccgacg tggaacgcaa cagcgatgcg
     4741 tccgtcgcct ccatcttcga cggactcaac tggctcgacc tgggctggga cgaggagtac
     4801 aagcagtcgg agcgcctagc gcttcaccaa aagactgccc agaccatctt cgagcgtggc
     4861 ctcgcctacc gcgacttcac ccccacccac ggcccggaag ccgaaaagtc cggcacccag
     4921 ggaacctggc tcttcaatcc cggcatgcgc gaactgtctc gatcggaaag cgaccggcgc
     4981 gccgcagcgg gagaaccgtt tgccctgcgc tttcgcgttc cccgcggaac tggagaggag
     5041 gttcgcttcc tcgatgcggt ctacggggac cagcgcaagc aggccgatgc cgtcgaggac
     5101 tttgccctgc tccgctccga cgggatgcct acttaccacc tggcctcctg cgccgacgac
     5161 gccgatctca gcatcaccca cgtcattcgc ggtcaggacc atctcaccaa caccttcaaa
     5221 catatcttga tctttcaggc aaccggcttc accccgcccc ggttcgcgca cctgccgctg
     5281 ctggtcgcgc ccgacggcac gaaactctcc aagcgccgcc acggccccgt cgtcagcgtt
     5341 accacctatc gcgacgccgg cttcctgccc gaggccttta tcaatttcct gtgcctgtta
     5401 ggctggtccc ccaaggacaa ccgcgaacgc atgagccgcg aggagctgat cgacgccttc
     5461 tccctcgaag gcatcaaccg cgccaacgct gtcgtgaact tcactgaaac gacggcggca
     5521 ctcgaggcca aagcgaccgc gggactggaa aaaaccgtct acccggaaga aaccttcgac
     5581 cccaaggccg tctggctcaa cgccgaatac atccgcgcct tgtctataga agacctcagc
     5641 gctcgcctgc tttcggtggt cgagggcgca ggctaccgcg tcgatccggc ccggatgctg
     5701 cgcatcacgc ccttgatccg cgagcgcatc aagtttctgt gcgacgtctt gaccgccgcc
     5761 gacttcttct tcgttgacca gctccccccc tacgatccgg cggagctcat cccgcaaaag
     5821 ggcgacgctg ccatggcgtt caaggttctg gagaaagccc gtgccgtcct cgccgccacg
     5881 gattttactc acgacgccct cgatcacgcc ctccgctccg cagcccagga gttgggcctc
     5941 aaggccggac agatgtttca gcccatccgc gtcgccgtct gtggccgcaa gaacgccccg
     6001 ccgctgtttg agacgttagc cgtcttgggc agtgacacga ccctggctcg catcgaccag
     6061 gccatgaaga aactgcagta gacgtcttca ggaaccatcc gggctggaca cctcgtgccc
     6121 cccccacggt gtgtgcaggc ccaagggcgc gaaggaaaat cggggaaggc tgaaccccgt
     6181 gtgcgcacag ctcgcgcgca cgtctctccc gcttcatcgg cctgcccgtc gggttgactt
     6241 catcttccct acctttccct acggctccgg tggcaacgct gtggccgccg ctggcttttc
     6301 gcggctgagg gcgccgcgcc acactcgatc agagtttcct aaaagagtcg agaccggaat
     6361 gactgcctac ggctttccgc ctggcctcca ctccggctgg aacgccgcgt tcaccagcca
     6421 ctcgaccgga gggatcagcg aacggatggc cgcggtcatg tgcgtgaagt cgatgtgggc
     6481 caggtcgtcg ctcggcttgt gataatcggc gtgcaacccg aaactcgaaa tcgtctgtgc
     6541 caccacgcct tttttcgcga gcagatagtt gtcggaacgc atgaaaaaat gctggtccgg
     6601 ccgcgcgtcg gccgcgagcc tggcgccgtg cgccgccagc gccgggccca ggttcgtccg
     6661 atcccacccg gtgagccaaa gcgtgtcacc agggtacttc gggtcgcgcc ggccgatcat
     6721 ctcgaactcc aggttggcgg cgatctccgc gagcgggacc ggcgaatgtg cgagaaacca
     6781 aagcgagccc agcccgcccg cttcttcgct gccgaatagc gcgaagatca ccgtgtgccg
     6841 tggtttcggt ccggaaccga gcacgcgcgc cagttccagc acggccgtgg ttcccgaggc
     6901 gtcgtcgtcg gccccgttgt agatgctgtc gcctttcacc ggttcgccca cgccgaggtg
     6961 atcgaggtgc gccgaaagca ggatcgaact tttgcgcagc tcggggtcgc ccccggtaat
     7021 cttgcccacg gcattccacg tcgagcccat ctccggcgct cccgtcttcg tctcgaggta
     7081 gatgtaagtg ccgtcgggaa taccccgcag gtcgatcagg gcctgctggc tgagcgcaaa
     7141 cacgttgaaa ctggatccca gccccgtctc cgattccccc tggagccggg cgggaagcct
     7201 gggcagttcg ctcccctttt cgtgccagtc ccggagcgtc tcggccgacg cgggcagcag
     7261 aaccgccagc gctccggcag cgagggcgcc gatcgccgtg tcgcgaatcc gctcggcgcc
     7321 gcctccgttg atgagcacga ccgcgtcttt gctaggctct tgcccgctcc cgggcgcgag
     7381 ctgttgaccc tcatgttccc gtgcttctgg ttcctgcgcc gcgcctagcg cccgcccctc
     7441 ctctctcaag gccggctcag gtccaaggat gatccgcttc aggcgtcctc gaaattcagt
     7501 gtccgatatt tgctcgacca gcatttcctt gccatgcgtc cagacgatgg tctgcgccgg
     7561 gatgccatcc ccgggcgtca tgaagtgcat ggtcggaggc gcagtcagga ccggtcggac
     7621 ggtgaccgcc cgctggatat aactgccgtt gtctcccgcc ggagcaatgt cgtactgctc
     7681 cagtgcagaa gccacgtagg ttgccgccac cagttcgtcg tgggtcccgc tgccccgtcc
     7741 ccgcagcgcg tcagaggcca ggaagtccat gtgcgcccgg atacacgcca cgcagactcc
     7801 ggaccgtgcc ggagtgggcc gcgccaacgg gccggaagcg gagggttgcc cggcggcccc
     7861 tgaggtggct ggagctgggg ggccttgcgc ccaggtcccc agggccaaca gaaagaacaa
     7921 ccaggggcgc aaggaattgc gaacgctgcg ttctgtcatg agagtgggga acgattatag
     7981 cggccatcgg cggcaaatcg cgcttggccg ctaggctgcg cgagcgccag acggtgtggc
     8041 agagtcgacc attgctgctc gatggctggc gacctctgct acaatcaagc tagctgactc
     8101 tcctctccga gaagggaccg tttcgcgagg ctgtgaagtc atggtgacgt ggaaactcca
     8161 actggttctg tcatttactg cactttttct gctggctttg gccttgggtt gtaacgggtt
     8221 tttcgtggac ccagtcctgg tttctatcgt ggttgcgcct cccacgccca ccttggtggt
     8281 gggcgggagc gggcagcaga tgaccgcctt tgggaccttc gatgatggtt cgcagaagaa
     8341 catcaattcc agcgtcgcct ggaccatgaa tcctcccggc tatgcgacca tcacggcggg
     8401 cggcctgctc accggtacct cacccagtcc ttcgacggtg acgctccagg catccgatca
     8461 ggccgtcgtt ggatcgacca ccttccaggt tttcagcaac atcacttcga tcaccgtaag
     8521 cccggccacg cagagcgtgt cggtaagcag cgggacgccc ttctgcctgc aagccatagc
     8581 ttcgacgagc gcggggtcgc aggacatcag cgcgactgcc acctggacgt ttacggatcc
     8641 caacaaccag acggtgggcg ggatcactaa gaccacgggg acttgcaccg gtcaggcttt
     8701 ccagatcgga accctcactc catccgagac actgccggtg accttgagcg tgaccgcgtc
     8761 cgcgcccgct tccggcgggg gaaccgtcac ctccaactca gtcaaggtcg gtttgacgca
     8821 gtaaggaaac tccgatcaag cgagctgctg gcaggggaga agcgcgcttc gcgaagtgtt
     8881 gcttgaacga tggcacccgt tttacagcta tcgcatctcc gtcccaacgg ggtgcatcga
     8941 aagtcgccgt catcgcatca gccgcatagc atgccccttg cggcgatccg gtccgattgc
     9001 aatctactct ggtgaaaggc ctcagactta atttgcgacc tatgccacgg ttcattcacc
     9061 tacgggctgc ttccataact gccgataagt tgtcttttag ataagttact ggaaactcgg
     9121 gtgagcccat tttcccaacg ggaaaacctg gtttgagcta tcgcatagaa tccctgccag
     9181 tggactcagc aagcgcatag atggccgccc ggaaccaagg cgtatatctg gcgcaatccc
     9241 gaaaccttgc tgacgctgcc atggaatcag agccgctatg tattatctct aagatattaa
     9301 atagcctaag gtcgcagctt tcaggtttca gatcgcgagg tcgggagtgc ggagggagcc
     9361 agctacgagt cttgagcccg gagctcgcag cgagcaacct gaaacggtgg gtgcaagctc
     9421 gaggtcgtga tctctgctct gatgctgggt cggtggcctt gggagctgct ccccgggacc
     9481 acgtcctttc gtgcgcgtct gacccagccc atcaccgccc actctctcag tagcacgtta
     9541 tagtgaatag aactccggtg aaaatctcga ggcaagcgcg ttgggtgagg ctcctgtccg
     9601 ccctgatgct ggtggggctt atgggcgcgg ccggttgtgg gcgaggcaaa ggccggtcgc
     9661 gggaagcgat gtatgtttcg gccaaccagg cctctctgcg agatcgtgta gccgcggtgt
     9721 acagccgaac cggtgaggtg aagaacgggg accgggtcga ggtcctcgac cgcgagcggc
     9781 ggttcgtcaa agtgcgtaca actaccggtg ctgaaggctg gatcgagcag cgctatttgg
     9841 tcggccagca ggtctacgat ggcttccaga aactcgccct tgcccaccag tccgacacgg
     9901 tacaggcccc aggcatcacc cgcaacgaga ccaatcttca catcgaaccg gggcgcgata
     9961 gcgaacatct ctaccagctg ccgcagggca cgaaaatttc cctgctcgag cggggcaccg
    10021 ccgaaagacc gaacgtcgcc gcgcccgcca gcgcgactcc gcgcaaggac accaaaccca
    10081 ccgggccctt gctggaggac tggtggctgg cacgggacgc cgaaggccac gtgggttggc
    10141 tgcttgcccg catgctggat gtcgatgtgc cgctcgaaat cgcgcagtac gccgagggcc
    10201 agcgctttgt cgctttcttc acgctcgacc aggtgaagga cggcgacaag caggtgccac
    10261 aatacctggc ggtgctcacc gaggccaagg atgggctgcc ttacgacttc gaccaggtga
    10321 gggtgttcac ctggaacctg aagaagcacc gttacgagac cgcttaccgg gagcatggtc
    10381 ttaacggtgt cctgccggtc accctgaccc aagagagctt cgacagggaa ggcgatctgc
    10441 cggtattcct gctgcgcgta agagacgacg ccggcaccgt agttgagcgc aagtacaagc
    10501 tcaacacgcc catcgtacgg cgtgtgctgg cgccgggcga ggtcgcgacc ccgtcggcag
    10561 ggaaaaagaa gaagaaaagg cggtagggag gggtgggcga caggtaggag ggatcgggtc
    10621 agcgctgacc ctattccccg ctggctcgat cgtcaccgcg cgcctatcca caaaagcagg
    10681 gctaccagca ggaagactgc ccaggacggg tggcggccgt gcacttccac taccgagaac
    10741 atgccgctca gcaggaacac taccgcgaag atatcagcca ctgcattggc cgttctgtca
    10801 ggcttgcgca cagcgatcag caggaacgcc gttccggcgc cgatccagac aatcgaagtc
    10861 agatgccagg taaagcgcag aatgcgccgg gtgtaaaact cgtcaccaaa cagcttggga
    10921 aggcctgtcc ggcggaagag tcgctgcagc aaggcgcgtt cgcccaggaa ggaatggacg
    10981 agagccaata aaatcagcag tgcgccggca actgcgaagt aaacattcat gtgagacgaa
    11041 cttcggactc cagatctcag gtcttacggt ggcgggtgcc cgtcacgcca agacttggag
    11101 ctcagaccgc agagttcgac accggatgac ctgattaaga gccatccgac ttcagcaaac
    11161 taccacgagt ttcggccggc gaaggccctg gcatgaaatc gcacgctgcc tggcagccgt
    11221 gtaccgatcc gtgaatacga aggcggcatg cctgttgcca gttgccgtgc tgaccaccac
    11281 ggacggacag ccagccactg tttccggctg catgcctggt tcccctgccg cgtctataat
    11341 gccgcactgc atttcctcgt aatgcgacac aacgatcggt ctatcggaga agtcatgcgc
    11401 ctcttgtccg ttccgctgat cttcccgctc gttctctgct cgggttcctt cgccgcgcag
    11461 tcgagttcca ccccgacttc tgatatgtcg aaatccaaga aaagcaaaac cgaagcggcg
    11521 cccgctccgc aaaccacagc tccgaaccca cccgccccgg cagcgcccga ggccagaccc
    11581 gagtccgcga aacccgccgc cggcggcaag gagggggaag aacactatga catgaccgag
    11641 gtcgcgccgg tggtcacgca tcaccaagta acgctcgacg gcaaggtgct gaagtatacg
    11701 gcaacggcgg ggcgtttgcc catcaaaagg gacgacggca acattgaggc cgagatgttt
    11761 ttcgtagcct acacgctcga cgggcaggag gctgcgaagc gcccgttgac cttcgccttc
    11821 aacggcgggc cgggttcggc cacggtgtgg ctgcacatgg gggcgctggg gccgaagcat
    11881 gtcgtgctcc agcccaacgg attcatgccc gccgctcctt accggatgga ggacaacccc
    11941 aacacgctgc tggaccgcac cgacctggtg tttgtcgatg ccattgacac cggcttcagc
    12001 cgggcagcca acgccgagat ggccaggaaa tttctgggcg tgaaaggcga cgtcgaggcc
    12061 ttcggggagt tcgtccggct ctacctcacc cgccacgagc gctggccgtc gccgctgttc
    12121 ctgtttggcg agagctacgg aaccacccgg gccgccggcc tctccgggta cctcgccgag
    12181 cggggcatcg ctttcaacgg catcgtgctg ctttcgatgg cggtagattt ccagaccctg
    12241 gagtgggcga aaaacaacga cgagccttac atcctgctgg tgccgagctt cgcctcgatc
    12301 gcggcttatc acaagcggct ggcgcccgac ctgatcgagg acatgaagcg gacccgcgag
    12361 gaggcccggc gctgggcgga gagcgactac gcgctggccc tggctaaggg cgacgggctc
    12421 acgcccgagg agcggcagaa gatcatcgac cagtacgcgc gttacaccgg cctgagcaag
    12481 cagctgatcg acgacgctaa cctacgcgtt gacgtcggcc agttcaccca caacctgctg
    12541 cttgaccaga agttgcgggt gggccggctg gacggccgct ttaccgggcc tgatccggaa
    12601 gggctgctgg acacccgctt ctacgacccg acagcttcgg cgatcctccc gccctatacg
    12661 gcggtcttta accactacgt acgcagcgag ctgggctacc aggcggatac gccatacaag
    12721 gtctttgcct ggaacgagga ggcctttcat aagtgggagt ggggagatgc ggagaaaggc
    12781 tttcccaata ccgcgccgtc gctgcgggcg gcgatggtaa aaaacccgta tctcaagatc
    12841 ctggtgatgg aaggccatta cgatctggcg actccctttg ccgcggccga ctacaccatg
    12901 gatcacctga acctggaccc cgcctaccgc aagaacattt cctatgcgac gtacgacgcg
    12961 ggacacatgg tgtacatcga cagcgtgtcg catgtaaaga tgaagaagga cgcggtcgaa
    13021 tttatagaga ggtgcctgga gacggccgcc agccgttagt gggcgcacga ctaccccggg
    13081 acctttgctt agctatagaa ttctgccacg ctctccacca cccattgctg ttcctcgtcg
    13141 gtgagttctg gaaatatggg cagggcaagc acttcgcgcg cggcgcgctc ggattcgggc
    13201 agatcgcctt cgcggtagcc cagccatgcg aacacgggct gcagatgtag cgggatgggg
    13261 taatagatct cggcgccgat tttgcgttcg ttcaggaaac cgcgaagcgc gtcgcgccgc
    13321 cgggcgcgaa tcacgtactg gtggaagacg tgatgggcgt gcggggcaac ggagaggatg
    13381 cggaccggag cgtcgtcgcc cggggaaagg agaccagcct gggtaaacag gcggtcgtag
    13441 ttggcggcgc gctcgcggcg gcagcggttc cagtcttcga ggtggggtag cttcacgcgc
    13501 agaatcgccg cctggatggc gtccagccgg ctgttccaac ccatgtcttg gtgcaggtag
    13561 cggcgccggc tgccgtggtt gcgcaggctg cgcatgcgtt cggccagctc ggggctgttc
    13621 gtggtcacca ggccggcgtc gcccatcgcg ctcaagttct tggtgggata gaagctgaat
    13681 gctgccgcca gccccagtga gccagcgcgg cgaccccgcc aggcggcgcc aaccgcctga
    13741 gccgcatctt cgatcagggg cacgtggaag gcgtcactga ggcggttgag agcatccatg
    13801 tcggcgcatt gcccatagag atgcacggga agcaacgccc gcaagttgtt tggcttaccg
    13861 gcgcgcagtt gggcctcaac ctgcgcagga tcgaggttca aggtgcgcgg atcgacgtcc
    13921 acgaataaag gccttgcccc ggcgcgtacg atcgccgacg cggaggcgaa aaagctgaag
    13981 gcggtggtga tcacgctgga gccgggccgg actccggcgg cggccagcgc gagccagagc
    14041 gcctcggtgc ctgaggcgca tcccacgctg ccggcggcgc cggtgtagac cgagaactcg
    14101 tgctccaggg ccgccgcttc ttcgcccaga atgaactgcc ccgaggcaca cacgcgctcc
    14161 accgcggcca gaatctcctc gcggatggcg gcgtactggc ggctcaggtc gagcaacgcg
    14221 accgcgtcga gcctacgcgc ggtctttgag gtccagggct gggtcacgtc aatgccaatc
    14281 ttagcaggca ggtcgcaggc ttcggcgtca agcttccagg cttgaagctg caccgttcaa
    14341 ggctcggcca gcacttgtgc actcggagaa ctctgccctg agcagccaaa tcagctgaag
    14401 gctgagaact aatggctttg agcttgatgg catggcctga tgagctaatt cgcaacccgc
    14461 tgaagcccaa agcccggagc ctcaacccgg cgcagacccc tcctgcttat gaagcgatag
    14521 gacaggtggt gggggtggtg tacggttttc atccagagcc aggcgccagt gggcgagggc
    14581 ggcaagcagg gcggcgaggt ccagaccgcc gtgtttgctg ggataggcgg cgagattgcg
    14641 gcaggcccgg ccgagcactg agcggcatcc tgcgaggttg ccggtggagt ggtggtgcag
    14701 cgccaccgcg acttggatca gcccctgcag gaacctgcgc tcggcgggcg gagcagcacg
    14761 ccagacctgc tcgagctcct cgtgggcggc gaagaaatca gcgcggttga acagagcgat
    14821 tccatgctca tagtgcggcc agaaactctc gcagtcgttg aacacaggtc attcgttcga
    14881 acggttcata cagtaaatca tagaatttga cctgcaaatc ttcaaatttc gggagctccc
    14941 ccctgccaga caagacttaa ctgtttataa acagatgact taacagcgat ctgttttggc
    15001 acaacagttg cgctcgtgta cgcggcgtgt tttgtattcg tgtcgccatt tttctaatgg
    15061 ctaattcccc ggaaggacgc ccccgaaaag tctggggata tcaagatttg caggagagag
    15121 aattatgatt caggccaata caagctccaa gctgctcgta ctcgcggttg cggtgttggc
    15181 ggcccttagc ctggtgccag gtgccttcgg gcaggcgatt agcggcagct tgacaggatc
    15241 ggtcaccgat cccaccgggg cggctgtgaa cgcggcgacg gtggaagtga ccaatatcgc
    15301 caccggcttc aaggccagca ccaagaccca ggcctacggc tcgtatcttt tcaccgagtt
    15361 gccggtcggc acctacaccc tgacggttac ggcggctaat ttcaaaactt cgaccctgtc
    15421 cggcattccg gtcgacctca acaaatcgaa caccgccaac gtgaaactgg aactgggcag
    15481 cagcaccacg acgatcgagg tgtccggcgt ggcaccgccg gttgatacga cgaccgccca
    15541 gctgcaatcc aacttcgatt cccgattcgc gcaggacttg cccattacca gcaatggcca
    15601 aggcgccggt gttttgaatt tgtcgatgct ggcaccgggc gtgtcgaatg cgaatgccat
    15661 gggtctcggc gtgggacctt cggtcggcgg tcagcgtccc cgggacaaca acttcaccgt
    15721 ggaaggtgtg gacaacaata acaagacggt taccgggtcc cttatcacga ttcccaacga
    15781 cgcggtggat gagttctcgt tgatctccaa ccagttcaac tccgaattcg gtcactcgtc
    15841 gggcggccaa ttcaacacca ccgtcaagag cgggacgaac tcctttcacg gttcggcgta
    15901 cgagtatttc aggaaccgca acctgaatgc cgtggaccag acgtacgtga accaggggtt
    15961 aacgtcgaac ccacgctttg acagcaaccg cttcggcggc actttcggcg gaccgatcat
    16021 caaggacaag ttgtttttct tcagcaactt tgagtaccag cctacggggc tgacggcggc
    16081 atcctcggga ggcgcatcca cgcctacggc ggcgggtctg gcggcaatcg gagcggaccc
    16141 taatctcaat gcgaccaatt ttggaatctt caagcaatac gtgccagttg ccgctacggg
    16201 aagtgcttgt gccacgttca acggcaccag taatggcgtt cctttcggtg ccggcttcaa
    16261 agctccggcc aacgggattt gcccggcagg ttcggtcgaa ctcggtgcgg tgacgatcac
    16321 gccgccggca tatgaaaact acacaaactt cgtgcagagc gtggatttca acttgtcggc
    16381 gaaagaccag atccgggggc gctatgtttt gaataatcaa accggaccgg acatcgctgc
    16441 gcaactgccg gttttcttcg tgagtctgcc catcaaatat cgcctcttca cggtgagcga
    16501 gtaccatacg ttctctccca ccctgttgaa cgaatttcgc ttcgggttca atcgcttcga
    16561 tcagacctac gcggtcgggc cacagaaata cccgggcctg gatgcattcc ctaacatcgt
    16621 gctggctgat ttgggctcgg ctggtcttaa catcggaccg gaccccaacg ccccgcagta
    16681 cacgattcag aatttctatg gtttcgtgga caacgccagc tggaccgtgg gcaagcacac
    16741 cctgaaattt ggcgctgagt accggtggta catttcaccg cagaacttca cccagcgctc
    16801 gcgcggggat tacgaataca actcgaccca gctttacctg gaagacttta ccccggacat
    16861 catcggccag cgcagcaccg ggagcaccac ctactatggg aaccagaatg cggtctattg
    16921 gtacgtcaac gattcctgga aactcaagcc caatctcacc ctcaatctgg gagtgcgcta
    16981 cgagtggaca tcaattcccc tgggcgaaca gcggcagtct ttgaacgagg tgtccaattc
    17041 gccgaatgta atcattccgc aggtgaacca gcccctcata ttctctgcac cccacgcgcc
    17101 cagggatcag tttgcgccgc gcgtgggaat cgcttattca cccggatcca agggaaatat
    17161 gtccatccgg gccgggttcg gcatggcgta cgataccctg tacgataaca tcggcattct
    17221 tgccgtaccg ccgcaggtag gatccacgaa caatgtcaac accaacctgc caccagcggc
    17281 cgacttcctg gcgaacggtg gtttgaaagg cgggggcagc ggcatcacgg tactggaccg
    17341 ggcggatgcg atggcgtcca cgtcttcttg gataccgccc aatgtcaagt atccctactc
    17401 gattaactgg aacctcggcg tgcagcgatc cttcgggaag aactacacgg cggacgtaaa
    17461 ctacgtcggt acacgcggca accacctgga tgtgcagaac attctgaact tccaatccgt
    17521 gattaccccg cagaccgcac tgccgacctt tttctcgatg ccgacgcagg ccgccctgaa
    17581 tgcgctgacc aacagcaatc tgaacgccct ggaacaactc aacatcattc ctgacaatct
    17641 gaacaacgtc gatgggccag gcatcgggtt caaccaaagc gtgctcaccg gtttcatccc
    17701 ctccggatgg tccacctaca acggattgca ggcccatgtc acgcgccgat tcagcaacgg
    17761 tttgaccctg gacggtgctt acacctggag tcacaccatc gacaacagca ccgcggactt
    17821 tcacagcacg gatctgactc cacgccgcca gcaggatttc tttgccttca cgccggaaaa
    17881 ggccacgtct gccctttcgc gcacgcagcg gctcacggtc gcagccgtct acaacttgcc
    17941 gttcttcaag aatggcaact ggttcatgaa gaacattgtg ggcaactggg tatttagccc
    18001 gatttacacc tatgagtcac cagagttcgc cacggtgcag gccgcccgcg acgcgaacct
    18061 caacggcgat tccgcgggcg atcgcggcat cgtcaatccg gtgggcttca aagggcaggg
    18121 cggcaatgta atgccattga gcgccgttag tggacccaac gcgggacaag ttgtagccta
    18181 cgtggcgact caagacccgg ctggcaccgt accgtgctcg tcagcggcac agtgtcccga
    18241 gttcattcgt accggcctgg gatcaatttc caacatgggc cggaacacgc tggcgaccta
    18301 tcacaccaac aactttgacc tcggtaccta caaggacctg cacctgacag aaagaatgta
    18361 tttccggctg ggggcgcagt ttgggaacct gttcaaccat ccgcagtaca ttccgggggt
    18421 caatcctggc ttcgggctgg gggtgaatga cgtgtactcg tacagctcgt gctgcacgac
    18481 ttccacgtac cggaactttg ccactccgga caaagcaaac ttcaacgatc ccaaactcac
    18541 ctttgccagc agcgcgcgga cgatgggtct ggttgtgaaa ttcatcttct aattgttctc
    18601 gacagcgaaa ctccaaggga ggcccgcaag ggcctccctt tcctgtctca ccaggtaagt
    18661 cgagcctcag tgcgagtcag gtccaaggct aagtgatccc tcttccggac gggcctccat
    18721 tttgtgaccg tccatgctgc gattcggagg ctttcaccgg accttctacc ggtgtgtgca
    18781 atcgtctcag gttgagactc ggtatctcac tgcgagcgga ttccagctct gccccttctg
    18841 cgattcactt ttgacacgct gtaagtgatt gagcaagaag gtcgtgtgcg attggcggat
    18901 ttggaagacg agttgcaccg ttttcctaca catccgagtg tctccgaaga aattgcagca
    18961 gtagcgttag ggagccaatt tcactgggag ttggtggagg ggcgatgaca cgagccaaaa
    19021 tctttcgaat cgtattgctg gtcgccgggc tgacggccac agccgcagg
//
LOCUS       DASPUL010000013         3484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1339, whole genome shotgun sequence.
ACCESSION   DASPUL010000013 DASPUL010000000
VERSION     DASPUL010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..202)
                     /locus_tag="VEJ00_00305"
     CDS             complement(20..202)
                     /locus_tag="VEJ00_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99624.1"
                     /translation="MTRKMLDVSLAGRAGTPPEVGTVGALLMGPDGVFIIGSDFLMDG
                     GVTAACWFGELAPRTA"
     gene            265..630
                     /locus_tag="VEJ00_00310"
     CDS             265..630
                     /locus_tag="VEJ00_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXW99625.1"
                     /translation="MVQYSETHLDASFAALSDATRRAVLEQLGRSDASITDLAGKFHM
                     TLTGMKKHVGVLERAALVTTEKAGRVRTCRLAMRALEEEAAWIERHRQLWSARFDELD
                     KIVEHLKTKEKTDARRKRR"
     gene            608..1111
                     /locus_tag="VEJ00_00315"
     CDS             608..1111
                     /locus_tag="VEJ00_00315"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXW99626.1"
                     /translation="MPEGKEGSSAATKRSTVVERKGDREFVVRRTFNAPAHVVFEAWS
                     KADLFKRWWVPKSVGMTLLSCDMDVRTGGAYRLVFRHPNFDQPMAFFGTYREVTPNKR
                     IVWSNEESDQGAVTTVTFEENAGKTLVTFHELYPTKAALDEALASSAEALPEQFAQLD
                     ELLAGRS"
     gene            complement(1374..1895)
                     /locus_tag="VEJ00_00320"
     CDS             complement(1374..1895)
                     /locus_tag="VEJ00_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXW99627.1"
                     /translation="MPLGLKRYYGQHDLHFITFTCHRRAALLRSVRARNLFVKVLGEV
                     REAYGFALVGFVVMPEHVHLLIGEPAKGTPSTVLQVLKQRVSRRLRQKRPSAAGQFQL
                     PFGAEHFVPQFWQRRFYDFNVWSRKKRIEKLGYMHMNPVQRGLVASPRNWPWSSYVWY
                     EGKDDGLIRIDPV"
     gene            2052..3098
                     /locus_tag="VEJ00_00325"
     CDS             2052..3098
                     /locus_tag="VEJ00_00325"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXW99628.1"
                     /translation="MVLIGCDFHPSWQQVCWLDVETGETGEHEVVHTPGEVEKFYRQF
                     AAPALIGLESTGNCQWFVELAEQLGHEVWIGDAAKIRASEVRQQKHDRRDAALLLRLL
                     REGRFPRIWTPSSEQRDARQLLIHRHKLVRLRAQVKNGLQHLAMNRGVQKKRKLWSAA
                     GQQALRELPLRPWAGRRREDLLKLLAMLDGQVAALDLAVKAAAEQDANARLLMSQPGV
                     GPITSLAFVLTMGDVRRFPRGKQVASYLGLIPREYSSGGHQRLGSISKQGNRFMRMLL
                     VEAAQVAVQCDPGFRNEYLHRRHSKPSGVAKVAAARKLAIRLYWMLRTQKRYPEIVRV
                     ESSPRVALVVRHGG"
BASE COUNT          703 a         1007 c         1130 g          644 t
ORIGIN      
        1 gcccctgccc ttcgaagtgc tatgcagttc tcggtgcaag ctctccaaac cagcaggcgg
       61 cagtcactcc gccatccatc aggaaatcgc tgccgataat gaaaacgcca tccggcccca
      121 tcaaaagtgc accgaccgtt ccgacttcgg gcggcgttcc cgcccggccg gccagcgaca
      181 cgtcgagcat tttcctggtc attccgccta acactttgtt ttcctcttgc acgccgcaag
      241 tggatctgct ataaactgaa ccatatggtt cagtatagcg aaacccacct cgacgcttcg
      301 ttcgccgcgc tgtcggacgc cacccggcgc gccgtgctgg agcagctcgg ccgctccgac
      361 gcttcgatca ccgacctcgc cggaaagttc catatgaccc tcacgggcat gaagaagcat
      421 gttggcgtgt tggagcgggc cgcgctggtc actacggaaa aggccgggcg cgtcagaacc
      481 tgccggcttg ccatgcgcgc gctggaagaa gaggccgcct ggatcgagcg gcaccgccaa
      541 ctctggagcg cacgcttcga cgagctggac aagattgtcg agcacttgaa aaccaaggag
      601 aaaacagatg cccgaaggaa aagaaggtag ttccgccgcc acgaagcgca gcaccgtcgt
      661 ggaacgcaag ggcgaccgcg aattcgtggt gaggcgaacg ttcaatgccc cggcgcacgt
      721 cgtcttcgaa gcgtggagca aagccgactt gttcaagcgt tggtgggttc cgaagtcggt
      781 gggcatgacg ctgctctcgt gcgacatgga tgtgcgcacc gggggcgcgt atcgcctggt
      841 gttccgccat ccgaacttcg atcaaccgat ggcgttcttc gggacgtaca gagaagtgac
      901 gcccaacaag cgcatcgtct ggtcgaacga agaaagcgat cagggcgccg tgaccacggt
      961 gacgttcgaa gaaaatgccg gcaagacgct ggtcaccttt cacgaactct accccacaaa
     1021 agcggccctc gacgaagccc tcgccagttc ggcggaagcc ctgcccgaac agttcgcgca
     1081 gctggatgag ttgctcgccg gcagatcgtg accgcaccgc ggccaccggc gtatggaagg
     1141 gatgaccatg acacacacga tcaccgccgc gctggccagt tcggcgcggc gtgaggacga
     1201 tatgctcaga cggcacgtgg cgcagccttt cccaaaggtt tttggtttca ggtggcccgc
     1261 gcttttcgac ggtgttgcct ttaggggtgg cccatccttc gcgcgggttg cgaagggtgg
     1321 ggaatttcgg ccccgcagtt cggccccgtt ttttgttcgg cttgctttcc ccttcatacg
     1381 ggatcgatgc gaattaaccc atcatccttc ccctcatacc acacatagga actccacggc
     1441 caattcctcg gcgacgcgac caacccgcgc tgcacgggat tcatgtgcat gtagcccagc
     1501 ttctcgatcc tcttcttgcg gctccagacg ttgaaatcgt agaagcgccg ctgccagaac
     1561 tggggcacga aatgctcggc gccaaaggga agttgaaatt gtcctgccgc ggacggtctc
     1621 ttctggcgca gccttcgcga cacgcgctgc ttgagcactt gcagcaccgt cgaaggcgtg
     1681 cccttggctg gctcgccgat cagcaaatgg acgtgctcgg gcatcaccac gaagcccacc
     1741 aaggcgaagc cgtaggcctc gcggacttcg ccgagaactt tcacgaacaa attgcgggcg
     1801 cgcacggatc gcaacagtgc cgcgcggcga tgacacgtga acgtaatgaa atgcaggtcg
     1861 tgctggccgt agtagcgctt gagtcctaga ggcataaagg cggggccgca aggcaaagct
     1921 gaaagcgggg ccgaaaagcc ccacccttcg cacaacccgc gaaggattga ttggataaga
     1981 acgaaggctc ccttcggaat ccagtattct tggtttggag gacaaagaaa ctgggaagaa
     2041 aggagccctc gatggtgctt attggatgcg atttccatcc cagttggcaa caggtttgtt
     2101 ggttggatgt ggagaccggg gaaacgggag agcacgaggt ggtgcatacg ccgggagaag
     2161 tggagaagtt ctaccggcag tttgcagcgc cggcgttgat cggcctggag tccacgggga
     2221 attgccagtg gttcgtggag ctggccgagc agttggggca cgaggtgtgg atcggagatg
     2281 cggcgaagat tcgcgcgagc gaagtgcggc agcagaagca tgaccggcgg gatgccgcgt
     2341 tgctgttgcg gctgctgcgc gaagggcgct ttccgcggat ctggacgccc tcgagcgagc
     2401 agcgcgacgc gcggcagttg ctgattcacc ggcacaagct ggtgcggctg cgggcgcagg
     2461 tgaagaacgg actgcagcat ctggcgatga accggggggt gcagaagaag cggaagttgt
     2521 ggagcgccgc gggacaacag gcgctgcgcg aacttccctt gcggccgtgg gccggtcggc
     2581 ggcgggaaga tctgttgaag ctgctggcca tgctcgatgg gcaggtggcg gcgttggacc
     2641 tggcggtgaa agcagcggcc gagcaggatg ccaatgcgcg gctgctgatg agccagccgg
     2701 gcgtggggcc gattacttca ctggcgttcg tgctgaccat gggagatgtg cgccgttttc
     2761 cccggggcaa gcaggtggcc agttatctgg ggctgattcc gcgggagtac agctcgggcg
     2821 gacatcagcg gctgggctcg atcagcaagc aagggaaccg gtttatgagg atgctgctgg
     2881 tggaggcggc gcaggtagcg gtgcaatgcg atccgggatt tcgtaacgag tatttgcatc
     2941 gccgccacag caagccgagc ggagtggcga aagtggcggc ggcgcggaag ttggcgattc
     3001 gactctactg gatgctgcgc acgcaaaagc ggtatccgga gatcgttcgt gtcgagagca
     3061 gcccgcgggt ggccctggtc gtccgccacg gcggatagac cgaaggattg attgggcgct
     3121 ctcgtcccgg cacgggccgg gattcgcata gaagaatcat ggtcgaagtg gcaggccgta
     3181 tcgatggttg gtggaacggt ttcgccggga agtgattcac cgtgagattt ccagagttct
     3241 ttgtcctcca actctctggc aaccacgcgg tgcgaaaaac accgtgcagg acaagtgttg
     3301 cttgacaacg ccttcgttct tatcgacggc cacccgccga catcgtcatc gctcggatag
     3361 ttaggccacc catccgcaag cagaagctac gccaccttat ccttcccttt tacaagtcca
     3421 ggaagttcgc tgccctcttc cagcccgggc catgcggcgg ccacgactcc ctcagcagct
     3481 gatg
//
LOCUS       DASPUL010000014         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1235618, whole genome shotgun sequence.
ACCESSION   DASPUL010000014 DASPUL010000000
VERSION     DASPUL010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1235618"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VEJ00_00330"
     CDS             complement(<1..1087)
                     /locus_tag="VEJ00_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99629.1"
                     /translation="MGRSDITANTSAGLRGVDRRVIGWFTLLVVAGGLAVLYPQTLAV
                     GFAVLVALGLGWLGIKQARRLGLELWQILLVIALSGYMILNYGFENIAIHLGGFPIIV
                     SYGLMYASLALALISKRQLIAKALQEPAILCMFGLLGLTFLHFVVDLPSYGLWAIRDA
                     TMCLDGLFMLLGLAWAMERNSIGFINKWLLVVFGVNMLYTFTLPWAEKFWSWSPESGV
                     FLQVPILGNFNGAGDLLLAGAVFCICVGSYVIKRPAWLISGLALGQFLGIAITQVRRM
                     YIATVVVLVILVLLGEAKKFAKLLVLLPAAIAVIVVATSLGGLEISGRIGPVNLSFFK
                     EHIRSITGAEDTPGSDPQSRIIMGNEAM"
     gene            complement(1124..2014)
                     /locus_tag="VEJ00_00335"
     CDS             complement(1124..2014)
                     /locus_tag="VEJ00_00335"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HXW99630.1"
                     /translation="MMKTYRLIHAKAARTKIRVPMVWSWHVGLKPQDVFMASYPRSGS
                     TWLRFMLFEILSGEEPGFRKLEDRLPEIQHQRGKAPILPNGGRLIKTHEQYRSDYKRA
                     IFLVRDVRDVLLSGYARAVEVGIADLVSKGDLDSFLMAFLQGSALQMGSWQEHSRSYL
                     DSPLAKNGNLLVVKYEELRKNPEPRLRQILEFVGITPNMQIIRKAIEDNTLAQMRKKE
                     DNARKAGETSILLSPHKSAGEDGRFVRKGSVGGWRSKLSDAQIRLVDEYAGEIMDRLG
                     YERGVSEEQPAESAVSAVSV"
BASE COUNT          479 a          692 c          646 g          435 t
ORIGIN      
        1 ccatggcttc gttgcccatg atgatgcggc tctgcgggtc gctgcccggt gtatcttcgg
       61 cgccggtgat gctgcgaatg tgctccttga agaacgagag gttgacgggg ccgatgcgac
      121 cggagatttc caggcctccc aacgaagtcg ccaccacgat aactgcgatg gccgcaggaa
      181 gcaggacgag gagcttcgcg aattttttgg cctcgcccag cagaaccaga atgacgagaa
      241 ccacgacggt cgcaatgtac atccgtcgga cctgggtgat tgcgatgccc aggaactgac
      301 ccagagccag accggagatc agccaggccg gccgcttgat cacgtagctg cctacgcaaa
      361 tgcaaaacac agcccccgcc agcaggaggt cgcccgcgcc gttaaagttt ccgagaatcg
      421 gcacctgcag gaacacgccg ctttccggag accatgacca gaacttctct gcccagggca
      481 aagtgaaggt gtacaacatg ttcacgccga acacgaccag cagccacttg ttgataaagc
      541 cgatgctgtt ccgctccatc gcccacgcca gccccaggag catgaacagg ccgtccagac
      601 acatcgtggc atcgcggatc gcccacagcc cgtaagatgg aagatcgacc acgaaatgca
      661 ggaacgtaag accgagcagt ccaaacatgc acaggatggc cggttcctgt aatgccttgg
      721 cgatcagctg ccgcttggaa atgagggcca gcgccagaga ggcatacatg agcccataac
      781 tgacaatgat cgggaacccg ccaaggtgga tggcgatgtt ctcaaatccg tagttcagga
      841 tcatgtaacc gctcaacgcg attactagca gaatctgcca gagttccagg ccgaggcgcc
      901 gcgcctgctt gatccccagc cagccaaggc ccaatgccac aagcactgca aagccaaccg
      961 ccagcgtctg cggatagaga accgccaggc caccggccac gaccagcagc gtgaaccagc
     1021 cgatgacgcg gcgatcaact ccgcgcaggc ctgcgctcgt gttagccgtg atgtcagacc
     1081 ttcccatgca attctctatc ggctctgtat tctggttcgt aaactaaacg cttacggcag
     1141 ataccgccga ttccgcgggt tgctcttctg aaactccacg ttcgtagccc agccggtcca
     1201 tgatctcgcc ggcgtattcg tcgacgagcc tgatctgggc gtcgctcagc ttgctgcgcc
     1261 aacctccgac gctgcccttg cgcacaaagc gtccgtcttc gccggcgctc ttgtgcggtg
     1321 agagcaggat cgaagtctca cccgccttcc gggcgttgtc ttctttcttc cgcatctggg
     1381 ccagcgtgtt gtcttcgatc gctttgcgga taatctgcat gttgggcgtg atgccgacga
     1441 attccagtat ctgccgcagc cgtggttcgg ggttcttgcg cagctcctcg tacttcacga
     1501 ccagcagatt gccgttcttg gccagcggag aatccaggta cgagcgcgag tgctcttgcc
     1561 aggagcccat ctggagggcg ctaccctgca ggaacgccat aaggaagctg tcgaggtcgc
     1621 ccttcgagac gaggtcggca atgcccacct ccaccgctcg ggcatatccg gagagcagaa
     1681 catctctgac atcgcgcacc agaaaaatgg cccgcttata gtcgctgcgg tattgctcgt
     1741 gggttttgat caggcggccg ccgttgggca aaatcggcgc cttgccccgc tgatgctgga
     1801 tctcaggcag gcgatcttcg agcttgcgaa accccggctc ttccccggac aggatctcga
     1861 acagcatgaa gcgcagccaa gtgctccccg aacgaggata tgaggccata aagacgtcct
     1921 ggggcttgag gccaacgtgc caggaccaca ccatcgggac gcgaatcttc gttcttgctg
     1981 ccttcgcgtg aattagtcgg taagtcttca tcatagttgt gtttccctca ctgtgaattc
     2041 tgaaaagatg attggatctg atcttgatgg gggatccagc tagtcattgg ctgagagtgc
     2101 gaacaggggc gcggcagcct cggaggtctt tcggcctttg cggcggacca taacgacggc
     2161 cgtcacgaaa gcgagcgttt ccgacagcgc gatgctccac acggctccgc tcaagcccag
     2221 ggcccaggtg agcggaatgc cgactgctac cg
//
LOCUS       DASPUL010000015         3334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_824377, whole genome shotgun sequence.
ACCESSION   DASPUL010000015 DASPUL010000000
VERSION     DASPUL010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824377"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="VEJ00_00340"
     CDS             complement(<1..1407)
                     /locus_tag="VEJ00_00340"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HXW99631.1"
                     /translation="MTVALNHETTATRHRDGAIVLDGHSLTIEDVVAIARDRQRVDLH
                     AGAISRINQCRSLLERKIRAREIMYGVNTGIGELADVVLTSEQVEKYQKYLLYSHAAG
                     CGEPASEENVRAAMLSRLNTHCWGHSGIRLEIVRTQVEMLNRGVTPVVCQKGSVGACG
                     DLSPMAQMAITLMGEGEVFYQGRRLPAKEGMAEAGIPTIVFRERDGLAAINGSNFISG
                     MGCLELYDAETWLRTQEVALAMTLEALNANMQAYDPRIHSVRGYPGAQECAENIRRLT
                     EGSELLKLPGKKVQDAYSLRSSPQVIGAAREAFKWIRYMLEIELNHAADNPTFFPDEE
                     LVLTGANFQGTPMAFALELLGMSVTTVAALSERRLNRLMNSHLSMGLPPFLTKGAGMF
                     SGLMLTQYTAGALVCENRVLSHPAATGSIPAAADQEDFVSMGMTTAIKTRQIIENAWH
                     VIAIELMAAAQAFDFRFPV"
     gene            complement(1454..2818)
                     /locus_tag="VEJ00_00345"
     CDS             complement(1454..2818)
                     /locus_tag="VEJ00_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99632.1"
                     /translation="MDKAGKFAADAILAIDTLWGGDVMCPSGTGRFIADSWFSDEPLP
                     TAYTLPAAARIRESGGVAGKNVDRAALDDYLAKVDVPAAIHGVRQEGSTIVGLRGQYL
                     SGLALCLETMWDLAREILGKGDPVPYERSVKASTGHAPEPSQPDAKRRRVEELLGRAG
                     YSSSGSSGILGAVDAWRRDRMVPMASVRALSTAVIAHFDRLSEQNLLPHLPRELARVP
                     RANIEFLPIKDAWFSGSMNYIGRDRTGDRRPKYEATYEINASLQISVPEFEQLISHEV
                     VPGHVTTFAYLQDMYVRGQMGFEATVLTMNTRAATLFEGIANNAILIAHGVTEVDQLP
                     DEDLQIGVLLALLQDDAKNEASYLTWGEKMPKEEVATILRRDYLVTEERADKLSGAWG
                     RHPLLGRMYLPAYRSGTERVAQLRRKYRSGEILPVLYGCAGLVDCETVEAACTQPAYT
                     GQPV"
     gene            complement(2818..>3334)
                     /locus_tag="VEJ00_00350"
     CDS             complement(2818..>3334)
                     /locus_tag="VEJ00_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HXW99633.1"
                     /translation="FGEAVPGDLRQVLVAPQASALKAVVSGFMSGQPAPYILFGLGAM
                     VTVVLEMLGLSSMVFALGIYLPLQLTTPILAGGFLSHLVNKRADKTGGERGKTIRERG
                     IILAGGLMAGGALGGVIGAALRVLPHFKEEWIQTPFYDNVPVSQTISALFFVGLCLYV
                     WFTSLKREKEM"
BASE COUNT          702 a         1018 c          927 g          687 t
ORIGIN      
        1 aaccgggaaa cggaaatcga aggcttgcgc cgccgccatt agctcaatcg cgatcacatg
       61 ccacgcattc tcgatgatct gtctggtctt gatcgcagtt gtcatcccca tcgagacgaa
      121 atcctcctga tcggcggcag cagggatgga cccggtcgcc gccggatggc ttagcacgcg
      181 attctcgcag accaacgccc cagccgtgta ttgcgtcaac atcaggccgg agaacattcc
      241 ggcgcccttg gtcagaaatg gaggcagccc catggacagg tgagagttca tcagccggtt
      301 caatctccgc tctgaaagcg ctgcaaccgt ggtcactgac atgcccagca gctccaaggc
      361 aaaggccatc ggcgttccct ggaagttggc accggtcagc acaagttcct cgtcgggaaa
      421 gaatgttgga ttgtctgcgg cgtgattgag ttcgatctca agcatgtagc gaatccactt
      481 gaatgcctca cgagcagcgc cgatcacttg cgggctcgat cgcagtgaat acgcatcctg
      541 aactttcttg cctggcaatt taaggagttc ggatccctcg gtcagcctgc gaatgttttc
      601 ggcgcattcc tgtgcaccgg gatacccacg cacagaatgg atacgaggat cgtatgcttg
      661 catgttggca ttcaaagcct cgagcgtcat ggcgagagca acttcctggg ttcttaacca
      721 cgtttccgca tcgtaaagct cgagacatcc cattccgctg atgaagttcg agccgttgat
      781 ggcagctagg ccgtcccttt cgcggaaaac gatcgtggga attccagctt cagccatgcc
      841 ttcctttgcc ggcagtcttc tgccctgata gaagacctcg ccctcaccca tgagagtgat
      901 cgccatctgc gccatgggtg aaaggtcccc gcacgcgcca accgatccct tctggcacac
      961 cactggcgtc acgcctcggt tgagcatctc cacttgcgtg cgaacgattt cgagtcgtat
     1021 gcccgaatgg ccccagcaat gcgtgttcag ccgggagagc atcgcggcgc gcacgttctc
     1081 ttcacttgca ggttcgccgc aacccgcggc atgggagtag agcaagtact tttggtactt
     1141 ctcgacctgc tcagaagtga gaaccacatc cgcaagttct ccaattccgg tgtttacgcc
     1201 atacatgatt tcgcgggcgc gaatcttgcg ttcgagaagg ctacggcact ggttgatacg
     1261 gctgatcgct cctgcgtgta agtccacccg ctggcggtcg cgagcgatgg ccacgacgtc
     1321 ctcaatggtc aaagaatgtc cgtcgagcac aatggcacca tctcgatgcc tggtagcagt
     1381 cgtctcatga ttcaatgcga cggtcataga gtcctcgttc gtgctagttt tgccgcactt
     1441 cgcaaacctt tgttcatact ggctgaccgg tataagccgg ctgtgtgcat gccgcctcca
     1501 ccgtctcgca gtcgaccaat cccgcgcagc cataaaggac gggaagtatt tcaccggaac
     1561 gatatttgcg tcgtagttgt gcgactctct ctgttccgga acggtacgca ggcaggtaca
     1621 tgcggccgag caggggatgc cgtccccagg cgccagagag cttgtcggcg cgctcctcag
     1681 tgaccaggta gtctcggcgc agaatggtgg ccacttcttc tttcggcatc ttttcgcccc
     1741 aggtgagata cgaggcttcg ttcttggcat catcctgcag gagcgcgagc agcacaccaa
     1801 tctgcaaatc ttcatcggga agctggtcca cttccgtaac cccgtgggcg atcaggattg
     1861 cgttgtttgc gattccctca aaaagtgtgg cggcgcgtgt gttcatggtc aacaccgtcg
     1921 cctcgaaacc catctggccg cggacgtaca tgtcctgcaa ataggcaaat gtggtgacgt
     1981 gtccgggcac aacttcgtga ctgatcaatt gctcgaattc cgggacggaa atctgcagag
     2041 atgcattgat ctcgtaggtt gcctcatatt tcggccttct gtctcctgtg cggtcccggc
     2101 caatgtaatt catggagccg gaaaaccagg catctttgat cggaaggaac tcgatgttgg
     2161 cgcgcggcac gcgagccaac tcgcggggaa gatgaggcaa aagattctgc tcgctcagac
     2221 ggtcgaagtg ggcaatcact gcagtactaa gcgcgcgtac tgaagccatc gggaccatgc
     2281 ggtcgcgccg ccaagcatcc accgcaccga gaattccgct cgagcctgac gacgaatagc
     2341 ctgctcgccc caacagctct tccacccgcc gccgcttcgc atccggctgg gacggctccg
     2401 gcgcgtggcc ggtcgaagct ttgacgcttc gttcataggg cacgggatcg cccttgccga
     2461 ggatctcccg tgccaggtcc cacatggttt ccaggcaaag tgccaaaccc gacaaatact
     2521 gaccgcgcaa gccgacaatc gtcgagcctt cctggcgaac gccatgaatg gcggctggaa
     2581 catccacctt cgccaaatag tcatccaggg cggcgcgatc cacattcttg cccgcaactc
     2641 caccggattc tcttatgcga gccgcggctg gaagagtata agcggtcggc agcggctcgt
     2701 ccgaaaacca ggaatcggcg atgaaccggc cggttccgga ggggcacatc acatcgccgc
     2761 cccagagggt gtcgatcgcg agaattgcat ccgcagcgaa ttttcctgcc ttgtccatca
     2821 catctccttt tctcttttca gtgaagtgaa ccagacgtac agacacaagc cgacgaagaa
     2881 caatgccgaa atcgtctgcg agaccggcac gttgtcgtag aacggcgtct gaatccactc
     2941 ttctttgaag tgcggcagca cccgcagggc agctccaatc acgccgccaa gtgcaccgcc
     3001 agccatcagg ccaccggcaa ggatgatacc tcgctcacgg attgttttac cccgctcgcc
     3061 gccggtctta tcggcgcgct tgttcaccag gtgcgagagg aatccgccgg ccaggatcgg
     3121 cgtggtcaac tgcagcggca aatagattcc cagcgcgaag accatcgagg aaaggccgag
     3181 catctcaagc actaccgtga ccatggcccc cagaccgaac aagatgtaag gagccggttg
     3241 gccactcatg aatcccgaga cgacggcttt gagcgcagaa gcctggggcg cgaccaggac
     3301 ctgccgcagg tcgccgggca cggcctcgcc gaac
//
LOCUS       DASPUL010000016         6162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_550326, whole genome shotgun sequence.
ACCESSION   DASPUL010000016 DASPUL010000000
VERSION     DASPUL010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550326"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VEJ00_00355"
     CDS             <1..625
                     /locus_tag="VEJ00_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HXW99634.1"
                     /translation="RKSLCVVVMLAAGCLLAQDLKPILLPQPQTDGGRPLMQALKERK
                     TTRDFSAEKLPSQILSNLLWAAFGVNRPDGKRTAPSAMNWQEIDIYVATSDGLFVYDA
                     RGNRLEPLLAQDVRPATGSQSFAATAPMDLVYVADLSKVGSTQDSELFTYADAGFIAQ
                     NVYLFCASEGLATVVRGSIDRVALAKMMKLRPEQKIILAQTVGYPKK"
     gene            1089..2453
                     /locus_tag="VEJ00_00360"
     CDS             1089..2453
                     /locus_tag="VEJ00_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HXW99635.1"
                     /translation="MAIATINPATGEVVKTFQPLTEAEIEKKLQLAVSTFKAERKTPF
                     AVRAKRMLKAAGILERDKDKFAHLMTLEMGKTYKSAVAEAVKCTTACRYYAENSERFL
                     ADEVVETGANKSFVRYLPIGPILAVMPWNFPFWQVIRFAAPALMAGNVGLLKHASNVP
                     QCALALESIFLEAGFPEGAFQTLLIGSAQVDALLNDPRVVAATLTGSEQAGIQVGVGA
                     AKRIKKVVLELGGSDPFIVMPSADLDAAVATAVLARVQNNGQSCIAAKRFIVAEPIAD
                     EFERKFVARMESLKIGDPFDEKTELGPLATPDGVKDLDADVQRTVKAGAKVLTGGHPL
                     KQPGNYYAPTVLTHVPKNSPAYAEELFGPVASIFRVKDQDEAIRLANDVRFGLGASAW
                     TNDDKERERFINELEAGMVFINKMVASDPRIPFGGVKASGHGRELGMVGMREFVNAKT
                     VWIG"
     gene            complement(2498..2863)
                     /locus_tag="VEJ00_00365"
     CDS             complement(2498..2863)
                     /locus_tag="VEJ00_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXW99636.1"
                     /translation="MGIMRNVERRREARIEIDLGLLVWGVDVKGERFLQEARARDISS
                     RGAMLSGLDVDLRAGDVVGVLYAGKKARFRVVWIRYDETGDKMQVALHRVATDECPWH
                     DLVSEDSIPCRLPNRPEAR"
     gene            complement(3176..3913)
                     /gene="rph"
                     /locus_tag="VEJ00_00370"
     CDS             complement(3176..3913)
                     /gene="rph"
                     /locus_tag="VEJ00_00370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HXW99637.1"
                     /translation="MFYRSDNRAPDQLRSVSFTPDFITTAEGSALIEVGNTRVICTAS
                     IEEGVPQFLRNTGKGWISSEYSMIPRSTLTRTPREVSKGRPSGRTHEIQRLIGRSLRA
                     VTDLTRLGERTIWIDCDVIQADGGTRTASITGAFVALGLALEKLVAADTLSAVPLRDF
                     VAAVSVGIVDGEILLDLSYEEDSRADVDMNFVMTAGYKMVEVQATAEHRVFDEQQLQK
                     MLSLARQGVQQLIARQQAVLSALTLRQ"
     gene            4094..4648
                     /locus_tag="VEJ00_00375"
     CDS             4094..4648
                     /locus_tag="VEJ00_00375"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXW99638.1"
                     /translation="MYGRCGSWWRGGIAAPFMGGGFPWGRGGRWGRFFGPGEIRLALL
                     SMLESGPKHGYELMKELEAKSGGIYKASAGAIYPALQQLEDEGMVTSDQTAGKRTYTL
                     TNAGRAELARESEPVRRIWARAHEAGTWVFGPEGVEMARPAAEVMKVAIRVAGRNWND
                     SAKIAKIREILERAKHEIEGLEKS"
     gene            complement(4752..5378)
                     /locus_tag="VEJ00_00380"
     CDS             complement(4752..5378)
                     /locus_tag="VEJ00_00380"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HXW99639.1"
                     /translation="MKWEVHDIDTVVPRHLVIALAVLIVAVLGLTLYALHMRKIATTP
                     AVSVDTRSLAPPVTGPMERVVLFVAHDEDGSLRPSSAQIPMPSGRQQRAEELLRALLS
                     IYLDKTSPHVLGAGADVRSVFLVDPGVAVVDVNSAFADTHRSGVLIEELTVASLIHTI
                     SANTPDILKVKILVDGKERDTLAGHADLSSYYDVSAVNQLATQLQAGQ"
     gene            complement(5375..>6162)
                     /locus_tag="VEJ00_00385"
     CDS             complement(5375..>6162)
                     /locus_tag="VEJ00_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXW99640.1"
                     /translation="AVVTVNSTTALMATFSNDGRTITLSPPSVAAQTAAQSPSAASAP
                     GALPASAQTTAPPAGAAPTAYRRYFAVVDASHGGDDHGETLTSTLLEKDVTVSLARSL
                     RQELESRGITTLVLRDSDANLSLDQRAVFANGDHAAIYIAVHAASAGRGVRVYTALLP
                     FGEDDRGPFRSWSIAQHAALPLSRTAAGAVAAELQRRQVPVRTLEAPLRPLNNVIGAA
                     IAVEVAPQGTDVSQITAPDYQQLVTSAVATAIAATRDQLGAAP"
BASE COUNT         1249 a         1778 c         1976 g         1159 t
ORIGIN      
        1 gaggaaatcc ctatgcgttg ttgtcatgtt ggctgccggc tgtcttttgg cgcaggattt
       61 gaaaccgatt cttttgccgc agccgcagac cgacggaggc cggccgctga tgcaggcgct
      121 caaagaacgc aagacaacgc gagacttctc cgcagagaag cttccttcgc agatactttc
      181 caatctcctg tgggccgctt tcggcgtgaa tcgaccggac ggaaaacgca ctgccccctc
      241 cgcaatgaac tggcaggaga tcgacatcta cgtagcgacc tcggacgggc tgtttgttta
      301 tgacgcaagg ggaaatcggc ttgaaccgct tctggcgcag gatgttcgtc cggccaccgg
      361 ttcgcagtca ttcgcggcta cggcgcccat ggatctggtc tacgtagccg atctctccaa
      421 ggtcggctca actcaggaca gcgagttgtt tacctacgct gatgccggtt tcatcgccca
      481 aaacgtatat ttgttttgcg cctcagaggg gctcgcgaca gtagtccgag gctcgatcga
      541 tcgcgtcgcc cttgccaaaa tgatgaagtt gcgcccagag cagaaaatca ttctggctca
      601 gaccgtcggc tatccgaaga agtagcgaaa tttcgctgat aggaatggcg ggatgagtgg
      661 cgcctgggga accacgttgt agtacgaatg gtatagtggt atgaccgctc gggttgcacc
      721 ttcgctgcga tcagcactat gagactcagg tgaccgacga ggaactattt cgccgcgaca
      781 caacatgcgt ccggcgtctt caccttgcgc tgggagaagc gatgccctgg catcgcgatc
      841 cgtatcggcg ggttgcagtg attctgaagg gtgatgttct ggccatcgaa taccgcgagg
      901 ggagccaacg gaacgggttg cggtacagcc gggtcaggtg gagtgggagg aaccgagcgc
      961 tcacattcac cgcgccgtaa acgccgggca acagccatac gaacaggtca cgatctttct
     1021 ccttgaccgg cccgacgccg tgccccagcc tttcgaagac taagcaagga gaagagggaa
     1081 gcgtcatcat ggcaattgca acgattaatc cagccacggg cgaagtcgtc aagacatttc
     1141 agccgctgac ggaagccgaa atcgaaaaga agctgcagct tgccgtaagc acttttaagg
     1201 ctgagcgcaa gactccgttc gcggtgcgcg ctaaacgcat gctgaaggcg gccgggattc
     1261 tcgagcgcga caaagacaag ttcgctcacc tgatgaccct cgaaatgggc aagacctaca
     1321 agtcggccgt cgccgaggcc gtgaagtgca ccaccgcttg ccgctactac gccgagaaca
     1381 gcgagcgttt tctggcggac gaagtggtgg agaccggggc caataagagc ttcgttcgct
     1441 atctcccaat cgggccgatt ctggccgtca tgccctggaa tttccccttc tggcaggtca
     1501 tccgctttgc cgctccggcg ttgatggccg gcaacgtcgg cctgttaaag cacgcctcga
     1561 acgtgccgca gtgcgcgctc gctctggaaa gcatctttct cgaggccggg tttcctgagg
     1621 gcgcgtttca gacgctgctg atcggctcgg cccaggtcga tgcgctcttg aacgaccctc
     1681 gcgtggtggc ggcaacgctg acgggcagcg agcaggcagg gattcaggtt ggcgtgggcg
     1741 cggccaagcg cattaagaaa gttgttctcg aactcggcgg cagcgatccg ttcatcgtca
     1801 tgccgagcgc cgacctcgac gcggccgtcg ctacagcggt gctggcccgc gtccagaaca
     1861 acggccagtc gtgcatcgcc gccaagcgat tcatcgtggc cgagcccatc gccgacgagt
     1921 tcgaacgcaa gttcgtcgcc cgcatggaga gcttgaagat tggcgatccg tttgacgaga
     1981 aaaccgaact gggaccgctg gccacgccgg atggcgtgaa ggatctcgat gctgatgtgc
     2041 agagaacggt caaggcgggc gcgaaggtgc tgaccggcgg acatccgctg aaacagccgg
     2101 gaaactacta cgcgccgacc gtgctgaccc acgtgcccaa gaattctccg gcgtatgcag
     2161 aagagttgtt tggccccgtg gcctcgatct ttcgggtgaa ggatcaggat gaggcgatcc
     2221 gtctggcgaa cgacgtccgc tttggcctgg gcgcgagtgc ctggaccaac gatgacaagg
     2281 agcgcgagcg cttcatcaac gaactggaag ccggcatggt gtttatcaac aagatggtgg
     2341 cttccgaccc acgtattccg ttcggcgggg tgaaggcgtc cggacacggg cgggaactgg
     2401 gaatggtggg catgcgcgaa ttcgtcaacg ccaagacggt gtggatcgga taacgcggca
     2461 gccaggcccg agcggctgtt tctttggccg gcctcgctta tcgcgcctcg ggacggtttg
     2521 gcagccggca gggtatcgaa tcctccgata ccagatcatg ccaggggcat tcatcggtgg
     2581 cgacgcggtg cagcgcgacc tgcattttgt caccggtttc atcgtagcgg atccacacca
     2641 cgcggaagcg cgctttcttg ccggcgtaga gcactcccac cacatccccg gcccggagat
     2701 cgacgtcgag accagacaac attgctccgc gtgagctaat gtcgcgcgcg cgggcttcct
     2761 gcaggaagcg ttcacccttc acgtccacgc cccagaccaa cagtccaagg tcaatttcga
     2821 tgcgggcttc gcgtcggcgc tcgacgttgc gcatgattcc catgaattgc gtatgaacca
     2881 gccaactgct tatagagcgg gcccgactgc gacgggacgg gccgaaaagc cttcactgtg
     2941 gcgacattgg cgagtgccag gccgtggttt gggaagcggc gctcgaggcc agctcgttga
     3001 ggtgcttgga ctcgcaatgc ccatcgctag ctccaaagta gcggggatga agacgggagt
     3061 aaaggttcct tagcaagact taacgagcaa tgcagctgcg gatttccgat tcgggagcga
     3121 tctacagcgg gaatgctgcg tccttctgtg cgtcaagacc cgcaacaggc gattctcact
     3181 gccgcagcgt cagagcgctc aagaccgcct gctgccgggc gatcaactgc tgcacgccct
     3241 ggcgggctaa ggacagcatc ttctgcagtt gctgctcgtc gaagacccga tgctcagccg
     3301 tggcctgcac ctcgaccatc ttgtagcccg cagtcatcac gaaattcatg tcgacatcag
     3361 cgcgcgagtc ttcttcgtag ctcagatcca gcagaatctc tccgtccacg atgcccacgc
     3421 tcacggcggc cacgaaatcc ctgagcggaa ccgccgacaa cgtgtccgct gctaccaact
     3481 tttccagcgc caggccgagc gcgacgaagg cgccggtaat cgaggcagtg cgcgtgcccc
     3541 cgtcagcttg aattacgtcg cagtcgatcc agatggtgcg ctcgcccagg cgcgtgagat
     3601 cagtgacggc gcgcaggctc cggccgatca ggcgctggat ttcgtgcgtg cgcccgctgg
     3661 gtcggccctt cgagacttcg cgtggcgtgc gcgtcaaggt tgaccgggga atcatgctgt
     3721 actcgctcga aatccatccc ttacccgtgt tgcgcagaaa ctgtggcacc ccttcttcga
     3781 tcgaagccgt gcagatcacg cgtgtgttgc ccacttcgat cagagctgat ccctcggcag
     3841 tggtgatgaa gtcgggagtg aaggagaccg aacggagctg gtcgggggca cgattatcag
     3901 agcggtagaa catggcgcga ttgtaaatca ttgaacggga acggaaaggc tatgattgac
     3961 acaagatata tctatagata ttattaacga cagatcgaac gatctgatct agactggaag
     4021 tcttgaaatc ctcaattccc aaagtcgaga tccaccggcg atctacaacc gagatttgaa
     4081 ggagttttga tttatgtacg gaagatgtgg aagttggtgg cggggcggca ttgcggctcc
     4141 cttcatgggt ggcggtttcc cctggggtcg cgggggccgt tggggacgtt tcttcggccc
     4201 cggcgaaatc cgtctggcgc tgctttcgat gctggaaagc ggacccaagc acggttacga
     4261 actgatgaaa gaactggagg caaaatcggg cggcatttac aaggcgagtg ccggggcgat
     4321 ctatccggcg ctgcaacagc tggaagacga gggtatggtg acctccgacc agacggcggg
     4381 caagcgcacc tacactctga ccaatgcggg ccgggcggaa ctcgcacgcg aatccgagcc
     4441 ggtgcgccga atctgggcgc gcgcgcacga ggcgggcaca tgggttttcg ggccggaagg
     4501 tgtcgagatg gcacgcccgg ccgccgaagt gatgaaggtg gcaatccggg tggcgggtcg
     4561 aaactggaat gattcggcga agatcgccaa gattcgcgaa atcctggagc gagccaagca
     4621 cgaaatcgaa gggctggaga aatcctagcc acgttccgac cggctgccag ggattacacg
     4681 tttctcgcgg atccggcacc aagactaccg tttcctgatc gtgagtatca ctgcgggccc
     4741 gtggcgtgat tttattgtcc ggcctgcaac tgggttgcga gctgattgac cgcgcttacg
     4801 tcgtagtacg aagacaagtc ggcgtggcct gccaaggtgt cacgctcttt cccgtcaaca
     4861 agaatcttca ctttcaggat gtcgggcgtg ttggccgaga tggtgtgaat cagcgacgca
     4921 acggtcaatt cctcgatcag cacgccggac cgatgggtat cggcgaaagc ggaattcaca
     4981 tcgacgaccg caactcccgg atcgaccagg aagacgctgc gcacgtcggc gccggctccg
     5041 agcacatggg gcgaggtctt gtcgaggtag atcgaaagca gggcgcgcag cagttcttcg
     5101 gcgcgctgct ggcgtccgga cggcatagga atctgagccg acgaaggacg caggctgccg
     5161 tcttcatcat gggcaacgaa gagcaccact cgctccatcg gcccggtcac aggcggcgct
     5221 agcgagcggg tgtcaaccga cactgcggga gtggtggcga tcttccgcat gtgcaacgcg
     5281 tacagggtca ggcccagcac agcgacaatc agcacggcca aagcgatcac caggtggcga
     5341 ggaaccacgg tatcgatgtc atggacttcc cacttcatgg ggccgccccc agctgatcgc
     5401 gtgtggcggc gatcgcggtt gccacagcgc tcgtcaccag ctgctggtaa tcgggcgccg
     5461 ttatctgcga aacgtcggtt ccctggggcg cgacttccac cgcgatcgca gcgccgatga
     5521 cgttgtttag agggcgaagc ggcgcctcca gagttcgaac cggaacctgc cgcctctgca
     5581 gttctgccgc gacggcgcca gcggcggttc ggctcagcgg cagcgcggcg tgctgggcga
     5641 tcgaccagga gcggaaggga ccgcgatcgt cctcgccgaa aggaagtagc gcagtgtaaa
     5701 cccgcacgcc tcgaccggcc gaggccgcgt gcacggcgat gtagatggca gcatggtcgc
     5761 cgttggcaaa gacagcgcgc tgatcgaggg aaagattggc atcggagtcg cgcagcacca
     5821 gggtggtgat gccgcggctc tcgagttcct ggcgcaggct gcgggcgagc gagactgtaa
     5881 cgtctttctc cagcaacgtg gaggtaagag tttcgccgtg gtcgtcgcct ccgtggctgg
     5941 cgtcaacgac ggcaaaatag cggcggtagg ccgtgggtgc ggcgccggcg ggaggtgcgg
     6001 tcgtctgtgc tgaagctggc aacgcgcccg gcgcagatgc tgccgacggc gactgagctg
     6061 cggtttgcgc agccacagag ggcggtgaca gcgtaatcgt gcggccgtcg ttgctgaacg
     6121 tcgccatcaa tgcggttgtc gagttcaccg ttaccactgc cg
//
LOCUS       DASPUL010000017         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_413699, whole genome shotgun sequence.
ACCESSION   DASPUL010000017 DASPUL010000000
VERSION     DASPUL010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413699"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VEJ00_00390"
     CDS             <1..503
                     /locus_tag="VEJ00_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXW99641.1"
                     /translation="PGAHGSNRTGRPFTGDASGNFMYPILYETGFANQPTATDRNDGL
                     KLKNLYITAAVRCAPPDNKPLPEELAACSHFLDRELAGLERVRVVVALGKIGFDAYLN
                     YLKRHGLLSLKKGFDFKHGASYQLPDGRVLLASYHPSNQNTQTGKLTQKMFLKIFEEA
                     AKLADR"
     gene            complement(635..1690)
                     /locus_tag="VEJ00_00395"
     CDS             complement(635..1690)
                     /locus_tag="VEJ00_00395"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HXW99642.1"
                     /translation="MLLQENVPLAPLTTLGVGGPARFFVEARSRTEVEEAIRLATARD
                     LALFVLGGGSNLVVADSGWPGLVLKVAILGIERLPGNENEGRILFDVGAGESWDRFVS
                     QAVVAQCAGVECLSGIPGSVGGTPVQNVGAYGQEVSETIESVEVFDLRDGQIRELCGE
                     ACGFAYRSSVFNTTERGRFIILKVTYALAPGGKPRLAYADLQRHFAGRESAPNLAEVR
                     EAVRHIRAVKGMLLVAGDPDCHSAGSFFKNPILSEAQHADLNKRAAARGLTIPSYPAL
                     EKSQKVSAAWLVEKSGFARGYGFGHVGLSSKHALAIVNRGAASAAEILALKDQLQQRV
                     EEIWGVHLEPEPVMVGF"
     gene            complement(1730..>2578)
                     /locus_tag="VEJ00_00400"
     CDS             complement(1730..>2578)
                     /locus_tag="VEJ00_00400"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HXW99643.1"
                     /translation="VSEDGKRAYVANSGSSNISVIDLEKRLVLGNVRVGAAPGLARVS
                     PDGSTVVVSNRGDNTVSLIDAKQLRVRATLAVCRQPEDIAILPDSSKAFVSCSGSSQV
                     ASIQLKKEHSQSGAQNDDHLLALLDVGRTPVSLALKPDGGEMIVCNFDSDSISIIETG
                     NDEVGSSFEIGQHPSHGVVTLDNSRLYVTNFGSNSVAVYDIDMGRRIATLTVGSRPDS
                     LALTPDQNYLLVLDTQSGDVTVIQKRTPRKLEPSEYSLLTLIPVGMQPNGIVVKAFMM
                     GTTKKK"
BASE COUNT          518 a          801 c          757 g          502 t
ORIGIN      
        1 ctcccggagc gcacggctcg aaccgcacag gacgcccgtt caccggcgac gccagcggca
       61 acttcatgta tcccattttg tatgagacag gatttgccaa ccagcctacg gccaccgacc
      121 gcaacgacgg actgaagcta aagaatctct atatcaccgc tgcagttcgc tgcgctccgc
      181 ccgacaacaa gcctcttccc gaggagctgg ctgcttgctc gcactttctc gatcgcgaat
      241 tggccggact ggagcgcgta agagtcgtgg tcgccctggg gaaaattgga ttcgatgctt
      301 atctcaacta tttgaagcgg cacgggctgc tctctttgaa aaaggggttt gacttcaaac
      361 acggcgccag ttaccagctt cccgacggca gggtgttact ggcgtcctat cacccctcga
      421 accagaacac tcagaccggg aagctgacgc aaaaaatgtt cctcaaaatc tttgaagagg
      481 cggcaaaact tgccgataga taagtcaagg cgccgctctg gatcgtcgca cccgcccctt
      541 ctcaggggtc acatcgaact ccggggcacg aaccggggag cggcgcctcc ttgtggccaa
      601 actgatgaca aagaaggaag ccgcagcgag ggttctagaa cccaaccatg accggctcgg
      661 gttccaggtg cacgccccag atttcttcga cacgctgctg gagctgatct ttcaaggcga
      721 ggatctcggc ggcactggct gcaccacgat tcacaatggc aagtgcatgc ttgctcgaaa
      781 ggcccacgtg gccgaagccg tatccgcggg cgaagcccga cttctcgacc agccacgccg
      841 ccgaaacctt ctggctcttc tcgagcgcgg gatagctggg aatcgtcagt cccctcgcgg
      901 cagctcgctt gttcaggtcc gcatgctggg cctcgctcag gattgggttc ttgaaaaatg
      961 atcctgcact gtggcagtcg ggatcgcccg ccaccagcaa catgcccttc acggcgcgaa
     1021 tatgccgaac ggcttcgcgg acttcagcca gatttggtgc cgactcgcgt ccggcgaagt
     1081 ggcgttgcaa atcggcgtag gccagccgag gcttgcctcc gggggcgaga gcataagtga
     1141 ctttcaaaat gatgaaccgc ccacgctccg tcgtgttgaa aacgctgcta cggtaggcga
     1201 acccgcaggc ttcgccgcac aattcgcgga tctgaccatc tctgaggtcg aagacttcaa
     1261 ccgactcgat cgtctccgac acttcctgcc cgtaagcgcc gacgttctgc accggcgtgc
     1321 cccccacgct gcccgggata ccactcaggc attcgacgcc cgcacattgc gccacgaccg
     1381 cctgcgagac aaagcggtcc catgattctc ccgcgcccac gtcgaaaaga atcctgccct
     1441 cgttttcgtt ccccggcagc cgctcgatgc ctaggattgc gaccttcaga acgagcccgg
     1501 gccagccgga atcggccacg acgaggttgc tgccaccgcc gagaacaaag agagcaaggt
     1561 ctcgcgccgt cgcgagccgg attgcttctt cgacctcggt ccgggatctg gcctcgacga
     1621 agaagcgggc ggggcctccg acgccaaggg tggtcagcgg cgccagcgga acgttttcct
     1681 gcagcagcat gggatcacta taccgagaga ctgttgccga gggcgaaacc tactttttct
     1741 tcgtcgtgcc catcatgaac gccttgacca caatgccgtt cggctgcatg ccgaccggaa
     1801 tcaaggtcag cagggagtat tcgctgggtt ccagtttccg cggggtgcgt ttttggatga
     1861 cggtcacatc gcccgattgc gtgtcgagta ccaaaagata gttctggtcg ggtgtcaggg
     1921 ccaggctgtc cgggcggctg cccacggtca gcgtggcgat gcggcgcccc atgtcgatgt
     1981 catagacagc aacgctgttg gagccgaagt tggtcacgta aagccgcgaa ttatcgagcg
     2041 taaccacccc gtgcgacgga tgctgtccaa tctcgaagct gctgccgacc tcgtcgttgc
     2101 cagtttcgat gatcgagata ctgtcggaat cgaagttgca cacgatcatc tcgccgccgt
     2161 ctggctttag agcgagactc acgggcgtgc ggccaacgtc gagcagggcg agcagatggt
     2221 cgtcgttctg cgcaccgctc tgggagtgtt cttttttcag ttggatggag gctacctggg
     2281 aggatcccga gcacgagacg aaagccttgc tcgaatcggg caggatcgcg atgtcttcgg
     2341 gctgccggca aacggcgagc gtggcccgta cgcgcagctg tttggcatcg atgagcgaca
     2401 cggtgttgtc gccgcggttc gaaaccacca cggttgatcc atcgggcgaa acccgtgcca
     2461 ggccgggcgc tgcgccgacg cgaacgttcc caagtaccag cctcttctcg agatcaatca
     2521 cgctgatgtt cgatgaccct gaattggcca cgtaggcgcg tttgccgtct tcagagac
//
LOCUS       DASPUL010000018         4210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1510769, whole genome shotgun sequence.
ACCESSION   DASPUL010000018 DASPUL010000000
VERSION     DASPUL010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510769"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VEJ00_00405"
     CDS             <1..548
                     /locus_tag="VEJ00_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXW99644.1"
                     /translation="PAVLSAQDTPETVLRPPKGSQVAIVIFEDLECPMCRRTSPLLEQ
                     ASKTYNIPVIRHDFPLPMHPWSYQAAVMARYFDTHSKAVGNEFRDYIFENQLEINVQN
                     LRSYAEKFAAAHKVDLPYVIDPQGKFAALVNADKSLGQAIRIDHTPTVYVVSSRNPNR
                     PYVEVKDNNQLYSTIDAVMKE"
     gene            complement(620..1594)
                     /locus_tag="VEJ00_00410"
     CDS             complement(620..1594)
                     /locus_tag="VEJ00_00410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXW99645.1"
                     /translation="MSGPSPNPESQRDRQELGAARFAMRLSLAVGVVMLLGKSAAYFV
                     TGSAAILSDFAESVVHVIAVAFAAFSLRLSAKPAAPTFLYGYERITFFSAGFEGAMIV
                     VAAIWILFEAITKWLAGLPLEHLGSGTLLLLGAGVLNAWLGWYLLRVGRRTHSLILEA
                     DGKHVLTDSLTSFGVVAGLGLVMLTGWKPFDPMVAIVVAANILWSGGWLAWRSAVGLL
                     DYSDPAAGEQIREKLDEICTELGVHYHGVRYRNTGYRQIIEVHLLFPQATSVGEAHRL
                     ATALEEGLPERLRAPAEVITHLESIEDHENVHSREHYTGRPGVGTADR"
     gene            1798..2490
                     /locus_tag="VEJ00_00415"
     CDS             1798..2490
                     /locus_tag="VEJ00_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXW99646.1"
                     /translation="MKPSIFVVEDDPDISRLVRHHLENEGFAVRVYPTGVNVLSDAER
                     QRPALFVLDIMVPGKDGLEICRTIRQTAGLASVPVIFLTAKSSEADRVLGLELGADDY
                     IAKPFSPRELVARVKAVLRRFERPLPPSPVKVGDIEIDPSAMTLTVRGNPVPTTATEF
                     RLLDYFARHKGRVFSRDHLLDSVWRDTSYVTSRSVDVYVRRIREKIEPDPENPRYLKT
                     VRGAGYRFEAPK"
     gene            2582..>4210
                     /locus_tag="VEJ00_00420"
     CDS             2582..>4210
                     /locus_tag="VEJ00_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXW99647.1"
                     /translation="MKNRIFFKLLTAFVIVIAATAVTLDFVIGNAWETSLRAEIERSL
                     TQKTLLFAHRVENDRNHTPLSEIAAQEGLAAGARATIIDASGKALADSEANPSTMENH
                     AARKEFAAALAGQTGSNERRSATLGIPFLYVAAPISGGAVRLAYPLSDVEAVQAQVRR
                     RLFWGAALALCVAMLVAALASVWTARRLDHIVDVAVRIERGDLRARVDDASLDEIGRV
                     AVAIDRTAGQVERSFAAVRSSQRQLETLLNSMQDAVIAVSSDGLVRWANQPMDRLVPQ
                     GTRLNAPVVETVRDPDFLSAVKAATSTKEVKTTRATSIIPGRAYDVTAAPLPDGGAVA
                     VLRDLTETERMEKTRRDFIANVSHELRTPLTSIQGYSETLLDSTSDNAGPTREFLEII
                     RKNAARMSRLTEDLLTLARVESGETRFETEPVPPVELLHDAEESFREIARGQGVELQI
                     IGLRNGGSGGSGSPPSGAAPTPSAPSGNGTVESLPRVLADREAIHQVFANLIDNAMKY
                     GRSGGRIVLGARRAPRGIEFYVKDFGAGISSEHLP"
BASE COUNT          810 a         1482 c         1167 g          751 t
ORIGIN      
        1 cgccggccgt gctttcagca caggacacac cggagaccgt cttgcgtccc cccaagggat
       61 cgcaggtcgc catcgtgatc ttcgaggacc tggagtgccc catgtgccgg cgcacctcac
      121 ccctgctgga gcaagcctcg aagacctaca acattccggt catacgccac gatttccccc
      181 tcccgatgca tccgtggtcg taccaggcgg ccgtcatggc gcgctatttc gacacccact
      241 ccaaggccgt gggcaacgag ttccgcgatt acatttttga aaaccagctg gaaatcaacg
      301 tccagaatct gcgctcctat gctgagaaat tcgccgcggc gcacaaggtg gatttgccct
      361 acgtgattga cccccagggc aagttcgcag ctctggtcaa cgccgacaag agtctcggtc
      421 aggccatcag gatcgatcac acgcccacgg tgtacgtggt gagcagccgg aatcccaacc
      481 gcccctacgt cgaggtcaag gacaacaacc agctttactc caccatcgac gcagtgatga
      541 aggagtagca atctttgatt gttgattggg agcgttgttg accgcggcat tgcgggagcc
      601 acgagctttt catcaagaat catcgatcag cggttccaac ccccggcctt ccggtgtagt
      661 gctcccgcga gtgcacattc tcgtgatcct cgatcgactc caggtgcgtg atcacttccg
      721 cgggcgcgcg cagccgctcc ggtagaccct cttccagcgc cgttgccagc cgatgcgctt
      781 ctccgacgga cgtggcctgc ggaaacagca gatgcacttc aatgatctgc cggtagccgg
      841 tgttgcggta gcgcacaccg tggtagtgca cgcccagttc ggtgcagatt tcatccagct
      901 tctcgcggat ctgctctccg gctgccggat ccgagtagtc gagcagccct accgctgaac
      961 gccacgccag ccacccgccc gaccacaaaa tgttcgccgc gaccacgatt gcaaccatcg
     1021 gatcgaaggg cttccacccg gtaagcatca ccagccccag ccccgcgacc acgccaaagc
     1081 tggtgagact gtcggtaagc acgtgcttgc cgtcagcctc gaggatcagc gagtgcgtgc
     1141 gccttcccac gcgcagcaag taccagccaa gccaggcgtt cagcactccc gccccgagca
     1201 ggagcaacgt ccctgacccc aaatgttcca gcggcagtcc cgccagccat ttcgtgatcg
     1261 cctcaaacag aatccagatc gccgccacca cgatcattgc gccttcaaag ccggctgaga
     1321 aaaacgtgat gcgctcgtag ccgtacaaaa atgtcggcgc cgccggtttc gcgctcagcc
     1381 gcaggctgaa tgcagcaaag gcaaccgcga tcacgtgcac caccgattca gcaaagtccg
     1441 acaggatcgc cgccgatccg gtcacaaagt aggccgcgct cttacccagc agcatcacca
     1501 cgccaacggc cagcgacagg cgcatggcaa aacgcgccgc gcccagttcc tggcggtcgc
     1561 gctgggattc cggattcggc gatgggccag acatcctggt accgctcatg ctgctgccga
     1621 tcatgctcac acgaagtgct cgagctggaa gtgactgcga tcacctctac gcgtcccagc
     1681 acctgattta gtgctgacaa tccggcattg ttacaatccg gtgaaaaacg cggcgtccgc
     1741 ggtaagacgc gccactcggc actaggtact cggtactccc ttcgctacaa tactgtcgtg
     1801 aaacccagca tcttcgtggt tgaagacgac cctgatattt cgcgcctcgt ccgccatcat
     1861 ctcgaaaacg aaggcttcgc cgtgcgtgtc tatccgaccg gggtcaacgt gctctccgat
     1921 gctgagcgcc agcgcccggc gctgtttgtg ctcgacatta tggtgcccgg caaggacggc
     1981 ctcgaaatct gccgcaccat ccgccagacc gccggcctcg cctcggttcc cgtcattttc
     2041 ctgaccgcca aaagcagcga ggccgatcgc gtgctcggtc ttgaactcgg cgccgacgat
     2101 tacattgcca agcccttcag cccgcgcgaa ctcgtcgccc gcgtcaaagc cgtgctgcgc
     2161 cgcttcgagc gcccgcttcc gcccagcccg gtcaaagtcg gcgatattga aatcgatcct
     2221 tcggccatga ctctgaccgt gcgcggcaat cccgttccca cgacggccac cgagtttcgc
     2281 ctgctcgatt atttcgcgcg ccacaagggc cgcgtcttca gccgcgacca cttgctcgac
     2341 tccgtatggc gcgatacttc gtacgtgact tcgcgctccg tcgacgtcta cgtccgccgc
     2401 attcgcgaga agatcgagcc cgatccggag aatccgcgat acttgaagac cgtacgcggc
     2461 gccggctacc gcttcgaagc gccaaaatga cgagagctac tgatcagtga aaagtggcca
     2521 gtgatcagag atggctcgcc aatcactgac cactaaccac tggtcactga ccacttagac
     2581 aatgaagaac cgcatcttct tcaagctgct gaccgcattc gtgatcgtca tcgctgccac
     2641 ggcggtcacg cttgactttg tcatcggcaa cgcctgggaa acctcgctgc gcgccgagat
     2701 cgagcgcagc ctgacgcaga agacgctcct cttcgcgcac cgcgtcgaaa acgatcgcaa
     2761 tcacactccc ttgagcgaga tcgccgccca ggaaggactc gccgccggag cccgcgccac
     2821 tatcatcgat gcttccggca aagcgctggc cgactcggag gccaatccct ccactatgga
     2881 aaaccacgcc gcgcggaaag aatttgccgc cgctctcgcc ggtcaaaccg gcagcaacga
     2941 gcgccgtagc gcgacgctcg gtattccctt cctctacgtc gccgctccca tctccggggg
     3001 cgcggtgcgt ctggcgtatc cgctctccga tgtggaagcg gttcaggcgc aggtccgccg
     3061 ccgcttgttc tggggcgctg cgctcgccct ctgtgtcgcc atgctggtcg cagcgctggc
     3121 ttcggtatgg accgcccggc gcctcgacca catcgtggat gtcgcggtgc gcatcgaacg
     3181 cggcgatctg cgagcccgcg ttgacgacgc ttccctcgac gagatcggac gcgtcgccgt
     3241 cgccatcgac cgcactgccg gccaggtcga gcgcagtttc gccgctgtgc gctccagcca
     3301 gcgccagctc gaaaccctgc tcaacagcat gcaggatgcc gtcatcgccg tcagttccga
     3361 tggcctggtg cgctgggcca accagcccat ggaccgcctc gttccccagg gcacgcgcct
     3421 caacgcgcct gtggtcgaaa ccgtgcgcga ccccgatttt ctctccgctg tgaaggccgc
     3481 cacttcgact aaggaagtca agaccacccg cgccacctcc atcattccgg gtcgcgccta
     3541 cgacgtcacc gccgcacccc tgcccgatgg cggtgccgtg gccgtgctgc gcgacctgac
     3601 cgaaaccgag cgcatggaaa aaacccgccg cgacttcatc gccaacgtct cccacgaact
     3661 gcgcaccccg ctcacttcca ttcagggata ttccgagacc ttgctcgaca gcacgtccga
     3721 caacgcgggc cctacccgcg agttcctgga aatcattcgc aagaacgcgg cccgcatgtc
     3781 gcgcctcacc gaggacctgc tcactctggc gcgggtggaa tccggcgaga cccgcttcga
     3841 gaccgaaccc gtcccgccgg tcgaacttct gcatgacgcc gaggaaagct tccgtgaaat
     3901 cgcgcgcggc cagggcgtag aactgcagat catcggattg cggaatgggg gatcaggggg
     3961 cagcgggtcg cctccgtctg gcgcggcgcc aacccccagc gcgccaagcg gcaatggcac
     4021 ggtcgaatcc ctgccgcgcg tgctcgcgga ccgcgaggcc attcaccagg tctttgccaa
     4081 cctcatcgac aacgccatga agtacggccg ctccggcgga cgcatcgtgc tgggagcacg
     4141 ccgcgccccc cgcgggatcg aattctatgt gaaggatttc ggcgccggca tctcttctga
     4201 gcatctgccc
//
LOCUS       DASPUL010000019         3829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_414052, whole genome shotgun sequence.
ACCESSION   DASPUL010000019 DASPUL010000000
VERSION     DASPUL010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414052"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VEJ00_00425"
     CDS             <1..538
                     /locus_tag="VEJ00_00425"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HXW99648.1"
                     /translation="SGNRATVVIHLAQLMATLVVKSDPPGANIYIDGRDMGAKTPAQV
                     SVDKGQHVVLVRKMGYIDETASMQFALGQTFNFSPTLRELANVDNIRTVGKMSKLFGG
                     KGVEAGQAKVTIHTQPKGAQVAINQHVLDKNSPVDVALDPGNYVVDITLTGYAPIHKV
                     ITVEKGGRIVIDEAMQHQ"
     gene            910..2505
                     /locus_tag="VEJ00_00430"
     CDS             910..2505
                     /locus_tag="VEJ00_00430"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXW99649.1"
                     /translation="MALALALVTALGPTTIPAFAAPARTFAPTATPIKHLVVIFQENV
                     SFDHYFGTYPIATNPSGEPFFKAASGTPTVNGLTNALLSFNPNLNPANGTGAANPFRL
                     DRSQAVTNDQDHDYTAEQLAFDAGLMDLFPLNTGTAGPPPSGSGVTQTNGLVMGYFDG
                     NTVTAEWNYAQHFAMSDNSYGTTFGPSSPGVINLVSGQTNGVIKTLNGTGDEVSGGPD
                     GSLTLIGDADPLGDVCSNPARAQAQLGSKNIGDLLSAAGITWGSFMGGFNLGIINPNG
                     TTGCHRSSANAVNGIATGDYIPHHSFFNYYASTANFAHTRPASLAEIGNGGPANHQYD
                     LEDFFIAAAAGNLPAVSFLKAQAFQDGHAGYSDPLDEQTLLVNTINFLELLPTWKETA
                     VVILYDDSDGWYDHQMGPIVNTSTGPADALSGPGACGTAATALPGLNSVTNPHALGRC
                     GYGPRLPLLVVSPWAKRNFVDHAVTDQSSVIHFIEDNWLGGQRIGQGSYDALSGPLNN
                     MFDFSHARTDGKLFLDPNTGERE"
     gene            2639..>3829
                     /locus_tag="VEJ00_00435"
     CDS             2639..>3829
                     /locus_tag="VEJ00_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXW99650.1"
                     /translation="MAAMALALRQAAPHGQVTKFRTQLDVSSLAPFVDPLPVPEVARA
                     TASRPSPADSAVQLPYYRIPMRQFETKFHRDLKPTRVWGYSSSSPGPTFETRSGQGLL
                     VEWTNELPDTHLFAIDHSLHGAEASKPQVRSVVHLHGGKAPPESDGYPENWYASGKSA
                     IYYYPNRQEAAMLWYHDHALGINRLNVFAGLLGTFFVRDDFEDALNLPRGKYEVPLVF
                     YDRFLDLDAQLNYPVSGDPEKPWVPEVFGNAILINGKLFPYLEVEPRKYRFRVLNGAN
                     GRFFHLSLSNGMVVQQIGTDQGLLPSPARLERITLAPGERADLVVDFAGQAGSRVMLS
                     NDDFAVMQFRVAREGPRDTSSLPVSLRPVPKIAEAAAVKTRTLSLVEVDDKLQRPVTM
                     LLNNA"
BASE COUNT          777 a         1224 c         1055 g          773 t
ORIGIN      
        1 ttccggcaac cgagcgacgg tcgtcattca tctggcccag ctgatggcca ctctcgtcgt
       61 caagagtgat cctcccggcg ccaacatcta tatagacggc cgcgacatgg gtgcgaaaac
      121 tccggctcag gtaagcgtcg acaaaggcca gcacgtggtg ctggtccgca agatggggta
      181 tatcgacgag actgcgagca tgcagttcgc gctgggccag acctttaatt tctccccgac
      241 cttgcgcgag ctcgccaatg tcgacaacat ccgaactgtc ggcaagatgt caaaactgtt
      301 cggcggaaag ggtgtggagg cgggacaggc gaaggtcacc attcacacgc aaccaaaagg
      361 cgcgcaagtg gcgatcaacc agcatgtgct tgacaaaaat tcgcccgtcg acgtggcgct
      421 cgaccccggc aactatgtcg ttgacataac gctcaccggc tatgcgccca ttcacaaggt
      481 catcaccgtc gaaaaaggcg gcaggatcgt cattgatgaa gcgatgcagc accaatagag
      541 tgactttctt atcacagcgc taagtctgct gcccgtctgc aagtgttctc ccgcccagac
      601 ctgctgactc ctgaccgcga gtttcacgat ctcgcactcg ccacctcacg tcatgcggtc
      661 gttatgtcag gcgaaaacct gtggataatc agtgcgaatg tcatgctgct gtaaagttca
      721 tttcaccttc ctttcattct cgtgcaactc ctgcgaaaca ttgtgacgct atcgttctct
      781 agaaccccgg ccgggaaacc attgtgaaaa gctgcgtcgt gtgtggcccc ctgctttcga
      841 gcactggccc gcggggacag gaggacgtta atgatggaac tgctgaagtc acacgccccc
      901 agaaaaacaa tggctcttgc gctcgctttg gttacagccc tgggaccgac cactattccg
      961 gcattcgcgg cgccggcccg cacgtttgct ccgacggcta cgccgatcaa gcacctggtt
     1021 gtgattttcc aggaaaacgt ctccttcgat cactacttcg gaacctaccc gatcgcaacc
     1081 aacccgtcgg gcgagccctt cttcaaggca gcctccggca cgcctacggt caacggcctg
     1141 accaacgcgc tcttgagctt caaccccaat ctcaatcccg ccaacggcac gggagctgcc
     1201 aatccgttcc ggctggatcg ctcgcaggcc gtcaccaacg atcaggatca cgactacaca
     1261 gccgagcaac tggcctttga tgccggactg atggatttgt ttccgcttaa cacggggacg
     1321 gccggaccgc cgccctcggg gtcgggcgtg acccagacca acgggctggt catgggttac
     1381 ttcgacggaa acacggtaac cgctgagtgg aactacgccc agcacttcgc catgagcgac
     1441 aactcttatg gcacgacctt tggaccgtcg agcccgggtg tcatcaatct ggtttccggc
     1501 cagaccaacg gcgtgatcaa gaccctcaac ggcaccggcg acgaagtgag cggcggtccc
     1561 gacggctctc tcaccctgat tggcgatgcc gatccgctgg gcgatgtctg ctccaacccg
     1621 gcgcgagctc aggcccagtt gggaagcaag aacattggcg acctgctgag cgccgctggc
     1681 ataacctggg gatcgttcat gggtggattc aacctgggga tcatcaaccc caacggcact
     1741 accggttgcc accgcagcag cgcgaacgcc gtgaacggca tcgccaccgg cgactatatt
     1801 ccccaccact ccttcttcaa ctactatgcc tcaaccgcga actttgctca tactcggccc
     1861 gcttcgctcg ctgagatcgg caatggcggc ccagccaacc accagtacga tcttgaggat
     1921 ttttttattg ccgcggctgc gggcaatctg cccgccgtca gcttcctgaa ggcgcaggcc
     1981 ttccaggatg gccatgccgg ttactctgat cctctggacg agcagacgct cctggtcaac
     2041 accatcaact tcctcgaact ccttcccacc tggaaggaga ccgcggtcgt gatcctctat
     2101 gacgactccg acggatggta cgaccaccag atggggccca tcgtcaacac ttccacaggc
     2161 cctgctgatg ccctcagcgg tcccggcgcc tgcggaacag ccgccacggc cttgcctggt
     2221 ctcaactcgg tgaccaaccc gcatgccctc ggccgttgcg gctacggtcc tcggctcccg
     2281 ttgcttgtcg tctctccctg ggcgaagcgc aacttcgtgg accacgcggt gactgatcaa
     2341 agctcggtga ttcactttat cgaggacaac tggctcggag gacagcgcat cgggcagggc
     2401 tcgtacgatg ctctctccgg ccctcttaac aacatgttcg actttagcca cgcccgcacc
     2461 gacgggaagc tgttcctgga tcccaatacc ggcgaaaggg aatagcagaa aactgtagaa
     2521 tcgtctgaac gactgacgcg ggcatgcgaa gaccagtgct ttgcatgccc gtccgattgt
     2581 tagctccggg agaatacctc tgtcccccac aagacgatcg tttcttcagc atggcggcat
     2641 ggcggcgatg gctttggccc tgcgacaggc cgcccctcac ggacaagtga ccaagtttcg
     2701 gacccagctg gacgtgagtt ccctggctcc gtttgttgat cctctcccgg ttcccgaagt
     2761 cgcccgcgcc actgcatcac ggcccagccc ggctgacagc gccgtgcagc ttccttacta
     2821 ccgaatcccg atgcgacagt tcgagacgaa gtttcaccgc gacctgaaac caactcgcgt
     2881 gtggggatac agttcgtctt cgcccggtcc cacgttcgaa acgcgcagcg gccagggatt
     2941 gctggtggag tggaccaacg agctcccaga cactcacttg tttgcgattg atcacagcct
     3001 ccatggtgca gaggcaagca agccacaagt ccggagtgtg gttcacctgc acggcggcaa
     3061 agctccgccg gaaagcgacg gttatccgga aaactggtat gcgtccggca agtcggccat
     3121 ctactactac cccaaccgcc aggaagcggc catgttgtgg tatcacgacc acgctctcgg
     3181 cattaatcgc ctgaatgttt tcgctggttt gcttggcacg tttttcgtgc gggacgattt
     3241 cgaggacgcc ctcaacctgc cccgtggcaa gtacgaagtc cctttggtgt tctacgatcg
     3301 tttcctcgac ctcgacgcgc agcttaacta tcccgtgtcc ggcgacccgg aaaaaccctg
     3361 ggtgccggaa gtgttcggta acgcaatcct gatcaacggc aagctgttcc cttatctgga
     3421 agtggagccc cgaaagtatc gctttcgcgt gctcaatggg gccaatggcc gcttctttca
     3481 cctgtcgctg tccaacggaa tggtggtcca gcaaatcggc acagaccagg ggctgctgcc
     3541 ttcgcctgcg cggcttgaac gcatcaccct tgcgccgggc gagcgcgccg acctggtcgt
     3601 tgattttgcc ggacaggcag gcagccgggt catgctgagc aacgacgatt ttgcggtgat
     3661 gcaatttcgc gtggcgcgcg agggtcctcg cgacaccagt tccctgcctg tgagcttgcg
     3721 gcccgttccc aaaatcgcag aagcggcggc ggtaaagact cggacgctga gtctggtgga
     3781 agtggacgac aagctgcagc ggcctgtgac catgctgctt aacaacgct
//
LOCUS       DASPUL010000020         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2833, whole genome shotgun sequence.
ACCESSION   DASPUL010000020 DASPUL010000000
VERSION     DASPUL010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2833"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..2000
                     /locus_tag="VEJ00_00440"
     CDS             66..2000
                     /locus_tag="VEJ00_00440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXW99651.1"
                     /translation="MRRSRSTLVLSVLLLFVIPTLVPAQTALIPRSVLFGNPARSSPE
                     ISPDGTMLAYLAPDEGVLNVWVRTIGKNDDRVITHDRKRGIRNFSWQFDSKNILYGQD
                     QGGDENWRIYQTSVATKQTKDLTPFEKSRADIVALEPSRPDTLLIQSNKRDPKVFDVY
                     RVNLKTGEINLDTQNPGDVAGWQPDHDLQVRAAQVTTDDGGTIIRVRDDSKSPWRELM
                     KWGPDETLGNIVDFSPDNKSLWIMTSLDANAARLLSVDIASGKHTVVAEDPQFDVSFA
                     VQQPKTNKLQAVVFLRERRDFQILDPDIQTDFDALRKVRDADISNISRDLKDDKWVVS
                     FEGDDAPVYYYLYDRASKQATMLFSNRPELEKYKLAKVKPVQYKARDGMTIYGYLTTP
                     AGSDAKNLPMVVFPHGGPWGRDLWGYDPYAQWLANRGYAVLQPNFRASTGYGKQYLNA
                     GDRQWAGAMHTDLLDGKDWVVQQGIADPKKVCIMGGSYGGYATLAGVAFSPDAFACGV
                     DIVGPSNLNTLLKTIPPYWSTLLAVFHKRMGDNEDFLKSQSPLFKADQIKVPLLIGQG
                     ANDPRVNKAESDQIVAAMRKNNKPVEYYVFPDEGHGFARPENRTAFNAASEEFLAKYL
                     GGRFEPSTDAEKKLLESVKQ"
     gene            complement(2102..2398)
                     /locus_tag="VEJ00_00445"
     CDS             complement(2102..2398)
                     /locus_tag="VEJ00_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXW99652.1"
                     /translation="MIRVVLPPHLRTLAHVTGEVTVEVESPVTQRSVLDALEARYPML
                     RGAIRDHVTLQRRAFLRFFACEEDWTHESPDAPLPEAVASGKEPFLVIGAIAGG"
     gene            complement(2395..2907)
                     /locus_tag="VEJ00_00450"
     CDS             complement(2395..2907)
                     /locus_tag="VEJ00_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99653.1"
                     /translation="MLQGGDRRSIGKSNQVVARVLRKQGEFEELIECLWSDDPIVCMR
                     AADAAEKISVRKPALLAAFKAELLGLADETTQAELRWHLALMLPRLRLTSRERARATL
                     RLQDYLNDRSSIVKTCALQGLAELAMRHAGREEEMIELLERVCRSGTPAMKARGRKLL
                     KHFQKQRKEP"
     gene            complement(2952..>3357)
                     /locus_tag="VEJ00_00455"
     CDS             complement(2952..>3357)
                     /locus_tag="VEJ00_00455"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXW99654.1"
                     /translation="NHYISIFKSGKILSVHRVEGKVLALTFELEGQQFMALNGGPLYK
                     FTEAISLFVDCDEQQEVDELWDKLSAGGEPGRCGWLKDRYGLSWQIIPKALMKLMGDA
                     DPAKSQAVLQAMMKMNKIVIAELQKAYDGKAA"
BASE COUNT          702 a         1098 c          868 g          689 t
ORIGIN      
        1 gaactccctc gacttgtttc tcgtatcgac ccctaggtag aatttgtctc ccacggaggt
       61 tgcccatgcg gcgttcccgt tcgaccctcg tcttgtccgt tctccttctg tttgtcattc
      121 ccacactcgt tccggcgcag accgccttga tcccccgtag cgtattgttc ggcaaccccg
      181 cccgcagttc tccggaaatt tctcccgacg gcacaatgct ggcctacctc gcgcccgacg
      241 agggcgttct gaacgtctgg gtgcgcacga tcggcaagaa cgacgaccgc gtcatcaccc
      301 atgaccgcaa gcgaggcatc cgcaattttt cctggcagtt cgattcgaag aacattcttt
      361 atggccagga tcagggcggc gacgagaact ggcgcatcta tcaaaccagc gtagccacga
      421 aacaaaccaa agatctcacg ccgttcgaga agtcccgcgc agacattgtg gcgctcgaac
      481 caagtcgtcc cgatacgctc ttgatccaga gcaacaagcg cgaccccaaa gtcttcgacg
      541 tctaccgtgt caatctgaag accggtgaga tcaacctcga tacccagaat cccggcgacg
      601 ttgccggctg gcagccagac catgacctcc aggtgcgcgc cgcccaggtc acgaccgatg
      661 acggcggcac catcatccgt gtccgggacg actccaagtc tccgtggcgg gaattgatga
      721 agtggggccc ggatgagact ctgggtaaca ttgtcgactt ctctcccgat aacaagtctc
      781 tctggatcat gacttcactc gatgccaatg cagcgcgtct gctctccgtg gacatcgcaa
      841 gcggcaagca caccgtggtc gccgaagatc cccaattcga tgtctcgttc gccgtgcagc
      901 agccgaagac taacaagctg caggcagtcg tctttctccg cgagcgccgc gacttccaga
      961 tcctcgaccc cgatatccag actgacttcg acgccctgcg caaggttcgc gatgccgaca
     1021 tcagcaacat tagccgcgat ctgaaggacg acaagtgggt cgtctccttc gaaggcgatg
     1081 acgctccggt ctattactac ctctacgacc gcgcgtccaa gcaggcgacg atgctgttca
     1141 gcaaccggcc cgaacttgag aaatacaaac tcgccaaagt gaagcccgtt caatacaagg
     1201 cgcgcgatgg catgacgatt tacggttatc tgaccacccc cgccggcagt gacgcgaaga
     1261 acttgccgat ggtcgtcttc ccccatggtg gtccctgggg acgcgatctg tggggatacg
     1321 atccctatgc gcaatggctg gccaaccgcg gctatgccgt gctgcagccc aatttccgcg
     1381 cttccaccgg atacggcaaa caatatctga acgccggcga tcggcaatgg gccggcgcca
     1441 tgcacaccga tctgctcgat ggcaaagact gggtcgtcca gcaaggtatt gccgatccga
     1501 aaaaagtttg catcatgggc ggaagctacg gcggttacgc cacactggcc ggagttgcct
     1561 tctcacccga tgcatttgcc tgcggcgtcg acatcgtcgg cccgtccaac ctcaacaccc
     1621 tgctcaagac gattccgccg tattggtcaa ctctgctggc agtcttccac aagcgcatgg
     1681 gcgacaacga ggatttcctg aagtcgcaat cgccgctgtt caaggcggac cagatcaaag
     1741 tcccgctctt gattggccag ggagcgaacg atccacgcgt caacaaagcc gagagcgatc
     1801 agatcgtcgc cgccatgcga aagaacaaca agccggtgga atactatgtc tttcctgacg
     1861 aaggccacgg cttcgcccgt cccgagaatc gtacggcctt caacgccgcc tccgaggaat
     1921 ttctggcgaa gtacctcggc ggcaggttcg aaccttccac ggacgcggag aagaagctgc
     1981 tggagagtgt gaagcagtaa gtggcttgag aaccagaaga aacgggtgcc ccatttctcg
     2041 cgcgttcttt gcgcgagaag cggggttttg cctgtcggga atctcgcggt ctagctaccc
     2101 actatccccc tgcgatcgcc ccgatcacca gaaacggctc tttccccgaa gccacagcct
     2161 cgggcaaagg cgcatcgggc gattcgtggg tccaatcctc ttcgcaggca aaaaatcgca
     2221 aaaacgcccg ccgctgcagc gtcacgtgat cccggatcgc gccgcgcagc atgggatagc
     2281 gcgcttccag cgcatccagc accgaccgct gcgtgaccgg actttcaacc tctaccgtca
     2341 cctctccggt tacgtgggcc agcgtgcgaa gatgaggcgg caatacaacg cggatcatgg
     2401 ttcctttcgt tgcttctgaa aatgtttcag gagtttccgg ccgcgtgcct tcatcgccgg
     2461 tgtgcccgat cggcatactc tctccagaag ttcgatcatc tcttcttccc ggcccgcatg
     2521 gcgcatcgcc agttctgcca gaccctgcag cgcacacgtt ttcacaatgg aactgcgatc
     2581 attgagatag tcctgcaacc gaagggtagc gcgcgctcgt tcccgcgaag tgagccgcag
     2641 ccgtggcaac atcaacgcca gatgccagcg caattccgct tgcgtcgttt cgtcagccag
     2701 tccgagcagt tccgccttga atgccgccag cagcgctggt ttccgcacag agattttctc
     2761 cgccgcgtcg gccgcccgca tgcagacgat agggtcgtcg ctccacaggc actcgatcaa
     2821 ctcctcaaac tcgccctgct ttcgcaggac cctcgctacg acctgattcg attttccaat
     2881 cgatcgccgg tcccctccct gcaacatccg acttaagcca gcgccgctca tgcaggtgcg
     2941 agcccgctcg cttacgctgc cttcccgtcg tacgccttct gcaactctgc gatgacaatc
     3001 ttgttcattt tcatcatcgc ctggagcaca gcctgcgact tcgctggatc ggcatcgccc
     3061 atcaatttca tcagcgcctt gggaatgatc tgccacgaca gaccatatct gtctttcagc
     3121 catccgcacc ggccgggctc tccgccggct gaaagtttgt cccatagttc atcgacttcc
     3181 tgctgctcgt cacagtcgac aaaaagcgag atcgcttccg tgaacttgta cagcgggccg
     3241 ccgttcaatg ccatgaactg ttgaccctcg agttcaaacg tcaaagccaa cacctttccc
     3301 tcgacacgat gaacgctcag aatcttcccg ctcttgaaaa tcgaaatgta gtggttc
//
LOCUS       DASPUL010000021         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_551766, whole genome shotgun sequence.
ACCESSION   DASPUL010000021 DASPUL010000000
VERSION     DASPUL010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551766"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..879
                     /locus_tag="VEJ00_00460"
     CDS             244..879
                     /locus_tag="VEJ00_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99655.1"
                     /translation="MATIDPEHERGRLAEFYTHQADGELERAAAQAYELTEAAREVLG
                     AEMTKRGLNAPMVPYPPGDEVELRNMVAIRRFRDLPEALVAKASLDSAGIDCALVDDN
                     VVRLDWFWSNLLGGVKLTVDAENVSAAEEMLGQPIPEHLDVAGVGEYEQPRCPKCGSL
                     DVNFQEIAPVAYVSAWLISMPIPVHRRAWRCHSCQVEWEDDGNPGPTESTA"
     gene            complement(868..1314)
                     /locus_tag="VEJ00_00465"
     CDS             complement(868..1314)
                     /locus_tag="VEJ00_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXW99656.1"
                     /translation="MQREFPTQPLIGVGAIIVEPGRTKDARVLLVKRAHPPIQGQWSI
                     PGGALEVGELVRDAAIREAREETGLIVEPGELLGVYDRVLRHTDGRVQYHYVLVDFLC
                     RVVGGKLHAAGDAAEARWFTRKELPALKLAEDTQEVIRKGFEKLGC"
     gene            1529..>2025
                     /locus_tag="VEJ00_00470"
     CDS             1529..>2025
                     /locus_tag="VEJ00_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HXW99657.1"
                     /translation="MALEEYKRKRRFEETPEPPPELAAKPGHRFVVQMHDATRLHYDF
                     RLEMEGVLKSWAVPRGPSLDPADKRLAMQVEDHPVSYFDFEGNIPEGNYGAGTVIVWD
                     VGTWEPLSPVAVQGKYVPGTEKEAAAMIAKGDLKFRLNGKRLQGDFALVKMKGRRPGS
                     KGNEWL"
BASE COUNT          400 a          556 c          661 g          408 t
ORIGIN      
        1 accggtgagt aaaggattga ttccgcctga cgcaggtcgg tcaggatcgt cgaaatgacg
       61 aaacccggag ttgccgtaga ggccacgtag agtgaccacc tcttggtcaa tcccaaacag
      121 tccaccgggc ggcccgcagg cgcaagcttg cgggccgttc tgttttcgga gaggctattc
      181 gccacggatt tgcgcggatt ttcacggatc acctttcgat cttcaggcgt atgatctctt
      241 gccatggcaa cgattgaccc tgagcatgag cggggacgac tagccgagtt ttacactcat
      301 caagcggacg gagaactgga acgggctgcg gcgcaggcat atgagctgac cgaggccgcg
      361 cgcgaagtgc tgggtgcgga aatgacaaaa cgaggcctaa atgctccgat ggtgccctat
      421 cctccgggcg atgaagtcga attgcggaac atggttgcta tccgccggtt ccgcgacttg
      481 ccggaagcgc tggtagcaaa ggccagcctg gattcagcag gaattgactg cgcgctggtt
      541 gacgataacg tagtgcggct ggattggttt tggtcgaatc ttctgggcgg agtcaagctg
      601 acggtggacg cagagaacgt ctcagcggct gaggagatgc tagggcagcc tattccggaa
      661 caccttgatg tcgcgggcgt cggagaatac gagcagcctc gttgcccgaa gtgtggatct
      721 ctggacgtga acttccagga gatcgcgcca gttgcctacg ttagcgcatg gttgatcagt
      781 atgccgattc cagttcaccg ccgagcgtgg cgctgccact catgccaggt cgagtgggaa
      841 gacgacggta atcctggtcc cacagaatca acagcctagc ttttcaaaac ctttgcggat
      901 tacctcctgc gtgtcctccg ccagcttcag cgccggtaat tctttgcgcg tgaaccagcg
      961 cgcttcggcg gcatcgccgg cggcgtgcag ttttcctcca accacgcgac acaggaaatc
     1021 aaccaagaca tagtgatatt gcacgcggcc gtcggtgtgg cgcaggactc ggtcataaac
     1081 gcccagcagt tcgccgggtt cgacgatcag tccggtttcc tcgcgggctt cgcggatggc
     1141 ggcatcgcgc accagttctc cgacttcgag ggcgccgccg ggaatcgacc attggccctg
     1201 aatcggaggg tgggcgcgct tgaccagcag tacgcgagcg tcttttgtgc gacccggctc
     1261 gacgatgata gcgccgacgc cgatgagcgg ctgggtggga aattcgcgtt gcatgctgca
     1321 cgcattctaa agtacgaatc tctgtgcata accttttcga tggcgtcatg ctgagcgcag
     1381 ccgttcttca ggcggagcga ggcatcggtg tttgagagcc gttttgacga ctgccgagct
     1441 tcgctctggc tggacgggcg aggcgcccgt ccctacctgg cctgattcgc tccgtgactc
     1501 cgtgtctccg tggttaaata gttcgcttat ggcgctcgaa gaatacaaac gaaagcggcg
     1561 gtttgaagag acgccggagc cgccccccga acttgcggcc aagcccgggc atcgcttcgt
     1621 ggtacagatg cacgacgcca cgcgcctgca ttacgacttc cggctggaga tggaaggagt
     1681 gctgaagtcg tgggcggtgc cgagaggtcc gtcgctcgat cccgctgaca agcggctcgc
     1741 catgcaggtt gaggatcatc ccgtttcgta tttcgatttt gagggcaaca ttcccgaggg
     1801 caactacggc gcgggcactg tgatcgtgtg ggacgtgggc acgtgggagc cgctttcgcc
     1861 cgtggccgtg caaggaaagt acgtgccggg aacagagaaa gaagcggcgg cgatgatcgc
     1921 caagggcgat ctgaagtttc gcttgaacgg gaagcggctg cagggcgact tcgcgctggt
     1981 caagatgaag gggcggcgtc cggggagcaa gggcaacgag tggct
//
LOCUS       DASPUL010000022         4956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_552096, whole genome shotgun sequence.
ACCESSION   DASPUL010000022 DASPUL010000000
VERSION     DASPUL010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552096"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /gene="trpS"
                     /locus_tag="VEJ00_00475"
     CDS             complement(<1..644)
                     /gene="trpS"
                     /locus_tag="VEJ00_00475"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXW99658.1"
                     /translation="MTASSKSRKRVLSGMRPTGKLHLGNYVGALQNWVRMQDDYECFF
                     CVVDWHALTTDYADTSSVKANSLEVVFDWLAAGLDPKKSVLFLQSHVPAHAELHLLLS
                     MITPLGWLERVPTYKEQRDNIKDKDLGTYGFLGYPVLQAADILVYKADTVPVGEDQVA
                     HVELTREIARRFNGFYGGEKGVFPEPQSLLTPAAKLPGTDGRKMSKSYGNSILMA"
     gene            complement(882..1091)
                     /locus_tag="VEJ00_00480"
     CDS             complement(882..1091)
                     /locus_tag="VEJ00_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99659.1"
                     /translation="MIGLLADVGSVSFPDLLSIIGSNVGLSGPVGLGDSSGGDSVGLS
                     GGIGMDYNAGTEYSVGLNGGVGLFA"
     gene            1277..2629
                     /locus_tag="VEJ00_00485"
     CDS             1277..2629
                     /locus_tag="VEJ00_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXW99660.1"
                     /translation="MTTLARKLRTIDYFTLGWGTMVGVGWLVVMDDWLLRGGSIGALL
                     GFVIGGALLLPIGWVYGKLVVAMPDAAGEIAYTSAVFSRPVSYATGWMMTLAYFIVCP
                     WEAVAVGRIAGYILPGLDSWELYRVAGRPIYLPHLVIGLALTALLTVLNYRGVRLSAT
                     FQNWTSFGTLALFIVFVALGVSRGSPRNLAPLFTHAPLVSILLVIQIVPYFMTGFESV
                     GKAAEEASSDFRPQGFYRAIWMAIFVGILFYACVVAAVGIVAPWRQLTAEKFMTAVAF
                     QQAVGARWIVNIILAAALLSLFKCFNGNFVAASRLVFALGRRGLVASGAGMIHERYQT
                     PSTAVLCVGLATAVCMLLGDAILVPITEVGSVACAIGWSAACAAYLALDGRSNTYVTP
                     SLIERGIAVVALLVGIAMALMKVIPLVPGHFTVYEWIALGVWIALGVISYRRPAIGRS
                     "
     gene            2718..4418
                     /locus_tag="VEJ00_00490"
     CDS             2718..4418
                     /locus_tag="VEJ00_00490"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HXW99661.1"
                     /translation="MKRNHALDRALFALFLLCALGRAVAQAGPAPFDLVITNGHIIDG
                     TGSPWYSGDIGIRDGKIAAIGNLAPAARKCTIDAAGKVVAPGFIDMLGQSELTILVDP
                     RLPSKIYQGITSEITGEGNSAAPLNDAIIQSDHTGYDHYKITPDWRTFREYFARLEKQ
                     GIGINVADYVGATQVRRMVLGDNDVQPTPVQLEQMKALVDEAMKDGAVGVSTSLEYAP
                     APYARTDELIALAAEAGKFGGIYATHMRNESDSVLEAIDEALRIGREAHLPVEIWHIK
                     VAGKDNWGRMPEVVAKINAARAAGADVTADTYAYTAWFNDFSAFIPPWAHDGGTAKLV
                     ERLKDPATRERIRKDLLTPSGDWDNEWQEIPGPDAIMIGAVQNPALLPLEGKRLSEIA
                     KLWNKEPMDALFNFLIQDPSTGVAVFGMSQPDVTLALQQPWVSIDNDSEGTSPDGILG
                     EAHPHPRAYGTFPRILSKYVRDEKVLALEDAIRKFSALPAQRMRLTERGVLKTGMWAD
                     IVIFDPATVHDRATFDNPNQLSEGMEYVLVNGVPVIDQGKMTGALPGKVLRGPGNVQQ
                     "
     gene            complement(4423..>4956)
                     /locus_tag="VEJ00_00495"
     CDS             complement(4423..>4956)
                     /locus_tag="VEJ00_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXW99662.1"
                     /translation="EVLGGKHQGEWAADFSATPAPCHGSGTLTGIALSQAADLMKDSW
                     ISGTTKAEYELSGACGAEFWQSAEGMVQFDVTSGSLPRISLGEGEGPLRFVHLAGLAH
                     LQVGKFEVKDARLDSTSGRYELSGTASLKRELALKLAAVPAGSRAGYAITGTLAQPRV
                     VPLPGAEQARLKPEPAK"
BASE COUNT          943 a         1587 c         1386 g         1040 t
ORIGIN      
        1 gccatcagga tcgaattgcc gtatgacttc gacatcttgc gcccgtcggt gcccggtaac
       61 ttcgcagctg gcgtaagcag tgattgcggc tcgggaaaaa cgcctttttc cccgccgtag
      121 aacccgttga acctgcgtgc aatctcccgc gtcagctcga cgtgcgccac ctgatcctca
      181 ccgaccggca ccgtgtcggc cttgtacaca aggatatcgg cggcctgcag cacgggatat
      241 cccagaaatc cgtacgttcc caaatctttg tccttgatgt tgtcgcgctg ttctttgtag
      301 gttggaacgc gctccaacca gcccagcggc gtgatcatcg acaacagcag gtgcagttcg
      361 gcatgcgccg gcacgtgcga ctgcagaaaa agcaccgact ttttcgggtc caatcccgcg
      421 gccagccagt caaacacaac ttcgagtgag ttcgccttaa cgctcgacgt atcggcgtaa
      481 tcggtggtaa gcgcatgcca gtcgaccacg cagaaaaagc actcgtaatc gtcctgcatg
      541 cgcacccaat tctgcagcgc gcccacatag ttgccaagat gcagcttgcc ggtgggacgc
      601 ataccgctga gcacgcgctt gcgcgatttc gaggaagccg tcattttctt atgaggccag
      661 gagatcggaa acacgagaag tgagaacgcg acctgggatg cagataagtt gtcgcagtgt
      721 catgtcaggc tggattcgat actaacacgc gaaacgccta tctgtgcggc gagatgggac
      781 gtgcattgca cgtgcgatta tgccacggta cgagagcatt gcagcgagat ctacatcacg
      841 ctctaagatt ccgccggccg gcagctcacg gggacaacct tctacgcgaa cagtcccacc
      901 cccccattca accccacgga gtattccgtt ccggcgttgt agtccatccc aattccaccg
      961 ctcagcccca cggaatcccc gccactcgaa tcgcccagcc ccaccggtcc gctcaacccc
     1021 acgttcgatc ctatgatgct gagcagatcc ggaaaactga cagagccaac atccgctaga
     1081 agaccgatca tacatttccc tcccggcgga tcacatcggc acagcccgca caggtgctta
     1141 ggtcatatcc gcaggattac agcagccatt tgcactccga tggctacgtc cctccggtgt
     1201 catccgccag ctaaagaaga aatgctttat aattcgcgcg cccaacggaa cagcccagcc
     1261 aggcccacca atcctcgtga ctaccctcgc ccgcaagctt cgtacgatcg actacttcac
     1321 gctgggctgg ggaacgatgg taggtgtcgg ctggcttgtc gtgatggacg actggctcct
     1381 ccgcggtggc tccatcggtg ctcttcttgg attcgttatc ggtggcgcgc tgctgcttcc
     1441 catcggatgg gtctacggca aactggttgt cgccatgccc gacgctgcgg gcgagatcgc
     1501 gtatacctcc gctgtatttt cccgccccgt gagctacgcc accggctgga tgatgacgct
     1561 cgcctacttc atcgtctgcc cctgggaggc ggtggccgtg ggccgcatcg ccggctacat
     1621 ccttcccgga ctcgactcgt gggaacttta ccgcgtcgcc ggtcggccca tctatcttcc
     1681 ccatctcgtc atcggactgg ctctgaccgc gcttcttaca gtgttgaact accgcggcgt
     1741 gcgcttgagc gccaccttcc agaactggac cagcttcggc accctcgcgc ttttcatcgt
     1801 attcgtcgcg ctgggcgtga gccgcggatc gccgcgcaat ctggcccctc tgttcaccca
     1861 cgctcccttg gtctcgattc tgctggtgat tcaaatcgtg ccatacttca tgaccggctt
     1921 cgagtccgta ggcaaagccg cggaggaagc cagctccgac ttccgccctc agggcttcta
     1981 ccgagctatc tggatggcca tcttcgtcgg cattctgttc tacgcctgtg tagtcgccgc
     2041 cgtgggaata gttgccccct ggcgccagtt gacggccgaa aaattcatga ccgcagttgc
     2101 gtttcagcag gcggtgggcg cgcgttggat cgtgaacatc attcttgcgg ctgcgttgct
     2161 atcgctgttc aaatgcttca acggaaactt cgttgccgcc agccgcctgg ttttcgcgct
     2221 tggacgccgc ggcctcgttg cctccggtgc gggaatgatt cacgagcgct atcaaactcc
     2281 gtccacagcg gtgctctgcg tcggtctggc gaccgcggtg tgcatgcttc tgggtgacgc
     2341 catcctggtt ccgatcacgg aagtcggctc ggtggcgtgt gcaattggat ggtccgcagc
     2401 ctgcgccgcg tacctcgcac tcgacggcag atccaatacc tatgtgacgc catccctgat
     2461 cgagcgcggt atagcggtgg tcgcattgct ggtcgggatc gcgatggcac tgatgaaagt
     2521 aatcccgctc gtccccggtc atttcaccgt ttacgagtgg attgctttgg gagtctggat
     2581 tgcgctgggc gtgatctcgt atcgccgacc ggcgattggc cggtcgtgaa gctttttttt
     2641 tcgcgctctt tgaggtattt ctcagcgagc tttgcggtta ttcttgcagt cagaagcatc
     2701 acccaggcgg gagaatcatg aaacgcaatc acgcactcga tcgagctctc ttcgcactct
     2761 ttttgctttg tgccctcggc cgcgcagttg cccaggctgg acccgcgcca ttcgacctcg
     2821 tcatcaccaa cggccacatc atcgacggca ccggctcacc ctggtactcc ggcgacatcg
     2881 ggattcgtga cggaaaaatt gcggcgatcg gcaatctcgc cccggctgcg cgcaagtgca
     2941 cgatcgacgc tgcaggaaag gtggtcgcgc ccggcttcat cgacatgctc ggccaatcgg
     3001 aactgaccat actcgtcgat ccgcgccttc cgtcaaaaat ctaccagggc atcacgtcgg
     3061 agattacggg tgagggcaat tcagccgctc cgctgaacga tgccatcatc cagtccgacc
     3121 acaccggcta cgatcactac aaaatcaccc ccgactggcg cacgttccgc gaatattttg
     3181 cgcggctgga aaagcagggt atcggcatca atgtcgccga ctatgtcggc gcgacccagg
     3241 tgcgccgaat ggttctcggc gacaacgacg ttcagcccac tccggtgcag ctcgagcaga
     3301 tgaaggcgct ggtcgacgag gccatgaaag atggagctgt cggcgtctct acgtcgctcg
     3361 aatatgctcc cgctccttat gcgaggaccg acgaattgat cgctcttgcc gcggaggccg
     3421 gcaagttcgg cggcatctac gctacccaca tgcgcaacga aagcgactcc gtgctcgaag
     3481 ccatcgatga agccctccgc atcggccgcg aggcgcatct gccggttgaa atctggcata
     3541 tcaaggtcgc cggcaaagac aactgggggc gcatgcccga agtcgtcgcc aagatcaacg
     3601 cagcacgcgc cgcgggcgcc gacgtcacag ccgacactta tgcctacacg gcttggttca
     3661 acgatttctc cgcgttcatt ccgccctggg cgcacgacgg gggtaccgct aagctggtcg
     3721 agcgcttgaa agatcccgcc acgcgcgagc gcatccgcaa ggatctgttg acgccttccg
     3781 gagactggga caacgagtgg caggagatcc ccggtcccga cgccatcatg atcggcgcgg
     3841 tgcagaatcc ggcgctgctt ccgcttgagg gcaagcgcct ctctgagatc gcaaagctgt
     3901 ggaacaaaga gcctatggat gctcttttca attttctgat ccaggatccg tccacggggg
     3961 ttgccgtctt cggtatgtcg cagcccgatg taacgctggc cctgcaacag ccctgggtct
     4021 cgatcgacaa cgattcggag ggcacgtcgc ccgacggcat ccttggcgag gcacatcctc
     4081 acccccgtgc ctacggaaca tttcctcgca tcctgagtaa atatgttcgc gacgaaaaag
     4141 tcctggcact tgaagacgct atccgcaaat tctctgccct gcctgcgcag cgcatgcgct
     4201 tgaccgaacg cggagtgctg aagacgggca tgtgggctga catcgtaatc ttcgatccgg
     4261 cgaccgtgca cgatcgcgct acgttcgaca atcctaatca gctttccgag ggaatggagt
     4321 acgttctggt caacggcgtg ccggtgatcg atcaggggaa gatgacgggg gcgttacccg
     4381 gcaaagtcct gcgcgggccg gggaacgtgc agcaatgagt attcacttcg cgggttcagg
     4441 tttcagccgc gcctgctccg cgcccggcag cggtacgact cgcggttgcg ccagcgtgcc
     4501 agtgatggcg tagccggctc gagatccggc tggaacagcg gctaacttca acgcgagttc
     4561 gcgcttcaac gaggctgttc cactcaattc atatcttccg cttgtggagt ccagtcgcgc
     4621 gtcctttacc tcgaacttgc ccacttgcag gtgcgccagt cctgcaagat gtacgaaccg
     4681 gagcggtccc tctccctccc cgagcgaaat ccgcggcagc gaccctgagg tgacatcaaa
     4741 ctgcaccata ccttccgccg attgccagaa ctctgcgccg caggcaccgc tcagttcata
     4801 ctccgctttc gtcgttcccg agatccacga gtctttcatc aggtctgcag cctgtgacag
     4861 cgctatcccg gtcagggtcc cgctgccatg acagggcgct ggcgtcgcgc tgaagtcagc
     4921 agcccactct ccctgatgct taccgccgag aacctc
//
LOCUS       DASPUL010000023         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1100598, whole genome shotgun sequence.
ACCESSION   DASPUL010000023 DASPUL010000000
VERSION     DASPUL010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1100598"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VEJ00_00500"
     CDS             <1..849
                     /locus_tag="VEJ00_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HXW99663.1"
                     /translation="TYGDSFIHVSDIDAFVESSRPLCALKKPIVTDLQVAIARNVASL
                     IDDGAVLQTGIGGIPDAVLPLLMDRKDLAVHSELVSDGVIPLIEAGVLTGARKNFKPR
                     KIILGFALGTKHLFDFVDNNPIFEFHPTAYTNDPGLIGRNDNMVAINSALQVDLTGQV
                     CSDSIGNQFYSGIGGQVDFLRGASRSKGGKPIIAISSTAKQGAISRIVPMLDPGAGVV
                     TSRGLVRYVVTEYGVAYLHGKSIRERAKALIEIAHPQFRDALYQYCEKTKWLQHPQPA
                     QFVAAK"
     gene            876..1172
                     /locus_tag="VEJ00_00505"
     CDS             876..1172
                     /locus_tag="VEJ00_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXW99664.1"
                     /translation="MSGPARGNVVIDVEECKGCGLCVESCPPKCLELAPELSAYGVHP
                     AHYTGHDCTGCGICFYCCPEPGAITVYRRLAPPKAVVDAKEQAVHAGGEHAPAL"
     gene            1120..2208
                     /locus_tag="VEJ00_00510"
     CDS             1120..2208
                     /locus_tag="VEJ00_00510"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HXW99665.1"
                     /translation="MRRSKLFMREANMRQLCKGNVAVIKGAVLAGCRAYYGYPITPAS
                     EIAEAAALYLPEVGGTFIQAESETASINMVYGAASAGVRVMSASSGPGLSLMQEGISY
                     LAGAELPCVIVDVMRGGPGLGNIAPEQSDYFAMVKGGGHGNYRNFVVAPASVQEMADL
                     TILAFELADKYRNPAIVLTDGFVGQMMEPLELEYSEIVPPEKPWAVKGTAETRKNMIS
                     SIFLEPDELEEHQRRMEAKYIRAQQTEARWEEHRTEDADLIVVGFGIVSRVLRSAVDE
                     ARKQGIRVGLFRPITLWPFPSKALEAVALRAGKVLVVELSNGQMVEDVRLALNGQRPV
                     EFYGRVGGNVPTVEELLAKVEEQVTANV"
     gene            2331..>2614
                     /locus_tag="VEJ00_00515"
     CDS             2331..>2614
                     /locus_tag="VEJ00_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99666.1"
                     /translation="MATHDTIPEEAKVSSNDYEVVHAKSPLFYPEYERKSDLKHQTHY
                     CPGCGHGVVHKLIAEALEDLGVQDQTILVSPVGCSVFAYYYFDVGNVQVA"
BASE COUNT          522 a          782 c          841 g          469 t
ORIGIN      
        1 acctacggcg acagcttcat tcacgtcagc gacattgatg cgttcgtcga gtcgtcccga
       61 ccgctgtgcg ccctgaagaa gccgatcgtg acggatctgc aggttgcgat tgcccgcaac
      121 gttgccagcc tgatcgacga cggcgcggtg ctgcagaccg gcatcggcgg aattcccgac
      181 gcggtcctgc ccctgctcat ggaccgcaag gatctggccg tccacagcga gctggtctcc
      241 gacggcgtca ttccgctgat cgaggccggc gtactcaccg gagcgcgcaa gaacttcaag
      301 ccgcgcaaga tcatcctggg attcgcgctc ggcaccaagc acctgttcga ctttgtcgac
      361 aacaatccga ttttcgagtt tcatcccacg gcctacacca acgatccggg gctgatcggc
      421 cgcaatgaca acatggtggc gatcaactcg gccctgcagg tcgaccttac gggtcaggtt
      481 tgttcggact cgattggcaa ccagttctac tcgggcattg gcgggcaggt ggactttttg
      541 cgcggagcgt cgcgctcgaa gggcggcaaa ccgatcatcg cgatttcttc gaccgcgaag
      601 cagggagcga tttcgcgcat cgtgcccatg ctcgatccgg gcgccggtgt agtgacttca
      661 cgcggtctgg tccgctacgt cgtcaccgag tacggcgtgg cctacctgca cggcaagtcg
      721 attcgcgagc gcgccaaggc gctgatcgag atcgcgcatc cccagttccg ggacgcgttg
      781 tatcagtatt gcgagaagac gaaatggttg cagcatccgc aaccggcgca gtttgtagcg
      841 gccaagtaag aagcagggaa gcgaggagag cgaacatgtc cggcccagca cgcggcaacg
      901 tggttatcga tgtcgaggag tgcaaaggat gcgggctttg cgtggaatcc tgcccgccga
      961 aatgcctgga actggctccc gagctgagcg cctacggagt gcacccggcg cactacaccg
     1021 gccatgactg cacgggctgc ggcatctgct tctattgctg tccggagccg ggagccatca
     1081 cagtctaccg gcgcctggcg ccgccgaagg cggtcgtgga tgcgaaggag caagctgttc
     1141 atgcgggagg cgaacatgcg ccagctctgt aaaggcaacg tggcagtgat caagggagcg
     1201 gtgctggccg gatgccgggc ttattacggt taccccataa cgcctgcgag cgagatcgcc
     1261 gaagctgccg cgctctatct tcccgaagtc ggcggcacct tcattcaggc cgagagtgag
     1321 actgcatcca tcaacatggt ttacggcgcg gcctcagccg gtgtgcgcgt aatgtccgcc
     1381 tcctccggcc cgggactcag cctgatgcag gaaggcatct cgtacctcgc cggcgcggaa
     1441 ctgccttgcg tgatcgtcga tgtcatgcgc ggcggccccg gcctgggcaa catcgcgccc
     1501 gagcagagtg actacttcgc catggtcaag ggcggaggcc acggcaacta ccgcaacttc
     1561 gtcgtagcgc cagcttcggt gcaggagatg gccgacctta cgatccttgc cttcgaactt
     1621 gctgacaagt accgtaaccc ggccatcgtc ctgaccgatg gtttcgtcgg ccagatgatg
     1681 gagcctctcg aactcgagta cagcgaaatc gtcccgccgg aaaagccctg ggcggtgaaa
     1741 ggcacggcgg agacgcgcaa aaacatgatc agctccatct tcctcgagcc cgacgaactg
     1801 gaagagcacc agcggcgcat ggaagcgaaa tacatccggg cgcaacagac cgaagcacgc
     1861 tgggaggagc accgcaccga agacgccgac ctgatcgtgg tgggcttcgg cattgtgtcg
     1921 cgcgtgctgc gctcggcggt cgacgaagcg cgcaagcagg gaattagggt cggcctattc
     1981 cggcccatca cgctgtggcc gttcccgtcg aaagcgctgg aagcggtcgc attgcgggcc
     2041 gggaaagttc tcgtcgtcga actgagcaat gggcagatgg tggaagatgt ccgcctggcg
     2101 ttgaacggcc agcgtccggt cgagttctac ggccgcgtgg gcggcaacgt ccccacggtc
     2161 gaagaactac tggcaaaggt tgaagagcag gtgacagcga acgtgtagtc ccggacgcat
     2221 tcgtccgggg cggttcttga aaggctgtgg tttgaaaggg cgcggcttca agccgcgccg
     2281 cagagaggtg ggtagagatg ggcggcttta gccgctgagg gagtaggtca atggcaacgc
     2341 acgacaccat tccggaggaa gcgaaggtga gcagcaacga ctacgaagtc gtacacgcaa
     2401 aatcaccgtt gttctatccg gagtatgagc gtaaatccga cctgaagcac cagacccact
     2461 actgccccgg ctgcggccac ggcgtggtcc acaagctgat cgccgaagct ctcgaagatc
     2521 tgggcgtgca ggatcagacc attctggtga gcccggtcgg ctgctcggtg ttcgcttact
     2581 actacttcga cgtgggcaac gtgcaggtcg cgca
//
LOCUS       DASPUL010000024         3934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1374893, whole genome shotgun sequence.
ACCESSION   DASPUL010000024 DASPUL010000000
VERSION     DASPUL010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1374893"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1546)
                     /locus_tag="VEJ00_00520"
     CDS             complement(<1..1546)
                     /locus_tag="VEJ00_00520"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HXW99667.1"
                     /translation="MADRNEIPTANSSAPGNLIATKIDPTSARFEANMRFLADLMSQV
                     HNEEETIREGGGAKAIERQHANGRLTARERIELLADAETFFELGLYAAHGMYEEWGGA
                     PAAGVITGMARVQTRLVMIIANDATVKAGAFFPMTAKKVIRAQNIAIENRIPTIYLVD
                     SAGVFLPLQEDVFPDTDDFGRVFRNNAVMSAMGIPQIAAIMGMCVAGGGYLPVMCDHV
                     LMTDGSGLFLAGPALVQAAIGQKVSAEELGGAAMHAGISGTVDFREPNDEACLARIRS
                     IVEKWGYRRLSPWDRKKPVDPELAAEEIYGIYDSSPARPYDMKEVLARVLDGSRFDEY
                     KPEYGKTLVCGYGRIGGFAVGIVANQKLHAQQTDHEGHKRIEFGGVIYTESAEKAARF
                     IMDCNQNLIPLIFFHDVNGFMVGRDAEWSGIIKAGAKMVNAVSNSVVPKIAVIVGGSF
                     GAGHYAMCGKAYDPRFVFAWPTARYAVMSGDSAAGTLVEIKVKQLERGGKKLSEDERK
                     ELYESVK"
     gene            complement(1631..2212)
                     /locus_tag="VEJ00_00525"
     CDS             complement(1631..2212)
                     /locus_tag="VEJ00_00525"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXW99668.1"
                     /translation="MTSSSFHLLREGDGILLRLQSRDGTNRLSRALLQDLHQAIIDLA
                     RENRPLVVTGSAHSFSAGAELGEIAALDGPAAYEFAKMGQTLMQLVQDFPAPVYAAIS
                     GYCMGGGLDLALACHRRIASPHAVFGHRGAALGLITGWGGTQRLPRLIGKGRALEMLV
                     AAERVSARRALQRGLVDAIAEDPVAEAIRRIRR"
     gene            complement(2214..2942)
                     /locus_tag="VEJ00_00530"
     CDS             complement(2214..2942)
                     /locus_tag="VEJ00_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99669.1"
                     /translation="MAAINTSRLREMLLNTVGPAPWYWQTFPSVHSRTGQRFAWTHHG
                     EQGPIGHLVTLGREERPERALLALNTYCRPFPAAPQLLGIWCPEGRKIRLTYFDPDQL
                     KEFDLIEVAGWFKQSSDRIYATTSPLADFEVPLNLGPGTHKIKVPAELQTLDELIVPT
                     SYKAMSPDDPAFALFVFYLQAGLVEVLPQKWFTSAQYQVGKQWITRAARDPESHRIFG
                     ECFGAGMFLLEDDGCRLAEWMGGV"
     gene            complement(2942..3361)
                     /locus_tag="VEJ00_00535"
     CDS             complement(2942..3361)
                     /locus_tag="VEJ00_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HXW99670.1"
                     /translation="MADFKIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQT
                     PEMIVNAALQEDVQVIGLSILSGAHNAIVPRVMDLLKQHKMDDVIVLVGGIIPDQDVD
                     GLKKAGVAAIFQPGTAMDEIVTFIRSHVKGPGVPAAG"
     gene            complement(3431..>3934)
                     /locus_tag="VEJ00_00540"
     CDS             complement(3431..>3934)
                     /locus_tag="VEJ00_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HXW99671.1"
                     /translation="EFHSLAECILYQQLNGKAAATIFQRFTGLAGDPLTPEGILKLTD
                     EELRSVGLSKQKSAYLRDLSEKTAAGLLDFARLADLPDQEVIQHLTQVKGVGVWTAQM
                     FLMFTLKRPDVLPTGDYGVQAAMKKHYNKRKLPKPHIMEKIAKPWAPYRSVACWYLWK
                     SLDIKTV"
BASE COUNT          771 a         1280 c         1076 g          807 t
ORIGIN      
        1 gcttcaccga ttcatagagt tcttttcttt cgtcctcgct caacttcttg ccaccgcgct
       61 ccagttgctt cactttgatc tcaaccaggg ttcccgccgc cgaatcgccg ctcattaccg
      121 cataacgtgc tgtcggccac gcaaacacaa agcgcggatc gtaagccttg ccgcacatcg
      181 catagtgtcc ggcgccgaac gatcctccga caatcacggc aatcttcggc accaccgaat
      241 tcgataccgc attcaccatc ttggcgccgg ccttgatgat tccgctccac tccgcgtccc
      301 gcccgaccat gaagccgttc acatcgtgaa agaaaatcag cgggatcagg ttctgattgc
      361 aatccatgat aaagcgcgcc gctttttccg ccgattcggt gtaaatcact ccgccaaact
      421 cgattcgctt gtggccctcg tggtcggtct gctgcgcgtg cagtttctga ttggccacga
      481 tgcccacggc aaatcctccg attctcccat agccgcagac aagcgtcttg ccgtactcgg
      541 gcttgtactc gtcgaagcgg ctgccgtcga gcactcgcgc cagcacctcc ttcatgtcgt
      601 acggacgtgc cggggatgag tcgtagatgc catatatctc ctccgccgcc agctcggggt
      661 cgaccggctt cttccggtcc cagggcgaca ggcgccggta tccccacttc tcgacgatgg
      721 atcgaatgcg ggccaggcag gcttcgtcgt tcggttcgcg gaagtcaacg gttccgctga
      781 tgccggcatg catcgccgcc ccgcccagtt cttccgcgga aaccttttgc ccgatcgctg
      841 cctgcaccag cgccggtcca gcgagaaaca acccgctgcc gtcagtcatc agcacgtgat
      901 cgcacatcac gggcagatat ccgcctcccg cgacgcacat gcccataatc gctgcgattt
      961 gcggaattcc catggccgac atcacggcgt tgttccggaa cacacgcccg aaatcatcgg
     1021 tgtcgggaaa cacatcttcc tgcagcggca ggaagactcc ggccgaatcc accagataga
     1081 tcgtcggaat gcgattctcg atggcgatat tctgggcgcg aatcactttc ttcgccgtca
     1141 tcgggaaaaa cgctcccgcc ttgaccgtgg cgtcgttggc gatgatcatc accaggcgcg
     1201 tctgcacgcg cgccatcccg gtaatcacgc ccgccgccgg cgctccgccc cactcctcgt
     1261 acatgccgtg tgccgcgtac agaccgagtt cgaaaaacgt ctcagcatcc gccagaagtt
     1321 caatgcgctc ccgcgcagtc aggcgcccgt tggcgtgctg cctctcgatg gccttggcgc
     1381 ctccgccctc gcgaatggtc tcttcctcgt tgtgcacttg cgacatcagg tccgccagga
     1441 agcgcatgtt ggcctcaaag cgcgccgagg taggatcgat tttcgtcgcg atcaagttgc
     1501 ccggcgccga ggaattcgcc gtgggaattt cgttgcggtc ggccatgtcg aaaatgaaag
     1561 tggtaagaaa aagcgaagca gcggactgtt tacggtatac cacgcaggca gaaccgcgca
     1621 aacgactgca tcagcgcctg attcgacgta tggcctcggc gaccggatcc tcggcgatgg
     1681 catccaccag gcccctctgc aacgctcgtc gtgcactgac cctctcggca gctaccaaca
     1741 tttcgagggc gcgccccttg ccaatcaggc gcggcagtcg ctgggttcct ccccatcccg
     1801 tgatcaaccc caacgccgcg ccgcgatgcc caaagacagc gtgcggcgag gcaatccggc
     1861 gatggcaggc cagtgccagg tcaagacctc cgcccatgca atatcctgaa atcgccgcat
     1921 acaccggagc agggaagtcc tgcacgagct gcatcagggt ttgtcccatt ttcgcgaatt
     1981 catacgccgc tgggccgtcg agcgccgcga tctctcccaa ctccgcgcct gccgaaaacg
     2041 aatgcgcact cccggtcact accagaggcc gattctctcg cgccagatcg atgatcgcct
     2101 gatgcaggtc ctgcagcagc gcgcgactca atcggttcgt tccatccctg gactgaagcc
     2161 gcagtaggat gccgtcccct tcgcgcaaca ggtggaacga tgaggatgtc aacctagact
     2221 ccgcccatcc actccgccag ccggcagccg tcgtcttcga gcagaaacat ccctgcgccg
     2281 aagcattcgc cgaagatgcg atgcgactcg gggtcgcggg cggccctcgt gatccattgc
     2341 tttccaacct ggtactgcgc ggaggtgaac cacttttgcg gcaatacctc gactagtcca
     2401 gcctgcaggt agaagacgaa gagcgcaaac gccggatcat cgggtgacat cgccttgtag
     2461 ctggtcggta cgatcagttc atcgagcgtc tgcagttctg caggaacttt gatcttgtgc
     2521 gttcccggcc ccagatttaa tgggacttca aagtcggcaa ggggagaggt tgtcgcgtaa
     2581 atgcgatcgg aggattgctt gaaccagccc gcgacctcaa tgagatcgaa ctccttcagt
     2641 tgatcaggat caaagtaggt cagccgaatc ttccgtcctt ccggacacca gattcccagc
     2701 aactgcggtg ctgccgggaa tgggcggcag tatgtattga gtgcaagaag agcccgctct
     2761 ggccgctcct cgcggccaag cgttaccagg tggccgatcg ggccttgttc tccatgatga
     2821 gtccaggcaa agcgttgtcc agtgcgagaa tggacggacg gaaatgtctg ccaataccag
     2881 ggcgccgggc ctacggtgtt caggagcatc tcccgcaggc gggaggtgtt gatcgcagcc
     2941 attacccggc cgccggcacg ccgggtcctt tcacatgcga acggatgaac gtcacgatct
     3001 catccatcgc cgtgcctggc tgaaaaatcg cagccacgcc cgccttcttg agaccgtcca
     3061 catcttgatc gggaatgatt ccgcccacca gaacgatcac gtcgtccatc ttgtgctgct
     3121 tgagcaagtc catgacgcgc ggcacgatcg cgttgtgcgc tccggaaaga atcgagagcc
     3181 cgatcacctg cacgtcttcc tgcagcgccg cgttgacgat catctccggc gtttgtcgca
     3241 gacccgtgta gatgacctcc atccctgcat cgcgaagggc gcgggcaatt actttcgccc
     3301 cgcgatcatg cccgtccagc ccgggcttgg ctaccagcac acgaatcttg aagtcagcca
     3361 ttgccaacat ttaaccacag agggcaaggg gaacatagaa ggacatcggc ttcggtcagt
     3421 gtccacgtgg ctaaacggtc ttgatatcca ggctcttcca cagataccag caggccacgc
     3481 tgcggtatgg cgcccagggc ttggcgatct tctccataat gtgcggcttg ggcagcttgc
     3541 gtttgttgta gtgtttcttc attgcagcct gcacgccgta gtcacccgtc ggcagcacgt
     3601 ccggacgctt cagtgtgaac atcaggaaca tctgcgcggt ccacactccg acgcccttaa
     3661 cctgcgtgag gtgctgaatc acctcctggt ctggaaggtc ggccagacga gcaaaatcca
     3721 gcagcccagc cgccgtcttt tccgacaagt cccgcaggta tgccgatttc tgctttgaca
     3781 agccgacgct gcgcagttcc tcgtctgtaa gcttcagaat cccttccggc gtcagcgggt
     3841 ctccggccag cccggtaaag cgttggaaga ttgtagctgc cgccttgcca ttgagctgtt
     3901 gatacaggat gcattctgcc aggctgtgga attc
//
LOCUS       DASPUL010000025        10139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_552343, whole genome shotgun sequence.
ACCESSION   DASPUL010000025 DASPUL010000000
VERSION     DASPUL010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10139)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10139
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552343"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="VEJ00_00545"
     CDS             complement(<1..990)
                     /locus_tag="VEJ00_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HXW99672.1"
                     /translation="MKKIPVFLLPVLCAVVLLAAVQPKFPPLPAAVTNNAVASLKGGL
                     ELYSLMGIGPKKTWDDVTSRVYVFHLSGAKWTEGRPVPGVAGRLGASAIGVRGQVFVF
                     GGYVVDPQGMEITVGDVNSYLADEHRWYRAEDIPVPVDSAVIGVDRDRYIYLVGGRSK
                     NGPVNNVQVYDAQKNTWSQATPFPGTPVYGAAGGLADGAIIYVDGAKKNTAFGAPYVE
                     SDECWTGKIDHKDPDKVEWAKLPPHPGPAHFGIVAGARGSKVIFSGGSPAVHNFKGLD
                     HEGKPVELSPVTFDFDVHGNRWETLDEETYDPRSDTRGMLSTPVGAVILGGMVS"
     gene            complement(1042..1512)
                     /locus_tag="VEJ00_00550"
     CDS             complement(1042..1512)
                     /locus_tag="VEJ00_00550"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXW99673.1"
                     /translation="MNLLDHLRRQFVYNAWANREVLTTIKGSGSDNPRALQLTAHIIA
                     AERLWLERIMQKPQSLPVWPDFDFERCEAESTEQARRWREFFDQTSAEDLSRLISYKN
                     SKGEPWDSTVEDILTHVAMHSAYHRGQIASHMRENGKIPAYTDYIHAVRQGLAR"
     gene            1681..2430
                     /locus_tag="VEJ00_00555"
     CDS             1681..2430
                     /locus_tag="VEJ00_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
                     /protein_id="HXW99674.1"
                     /translation="MPRKALTTSQTPDVETNAVEQPSRGTAGKTPAATRAGGPDDLAR
                     LALKACLIARDAAFNVSDLLTHSSRMAFLAIKDCEKELDLIERQIDDRLPAAITRVNE
                     HRARQLLSSLKSTTDLERIGDLVMSVAMRVQARTTKIPASDVRQLVEMSSVLRDMLDL
                     IHKGFSTLDLECARKVLQMDKDIDRVCHALFQKHLAETVAQGDTSGFEVLLMAQALER
                     AGDHTTNLAEDLYSLIEGRTLRHAPKKKAAT"
     gene            2593..3345
                     /locus_tag="VEJ00_00560"
     CDS             2593..3345
                     /locus_tag="VEJ00_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXW99675.1"
                     /translation="MQLPAEFCETTDRSSNVAALRPVVFVVEDDEDIARLISHNLQQA
                     GYHVQSFVSSASVLSEAVRQVPALFLLDIMLPGTDGFELCRHIRQTSVLSAIPIIFLT
                     AKTAEADRVKGLELGGDDYITKPFSPRELVARVRSVLRGLGQTPQDSEVMRLGDLQID
                     IASMTVTVQGRNVVTTVREFRLLEYLATHRGRVLTRDQLLDAVWKETPFVTPRSIDVY
                     IRRLREKIESDPRRPEYLKTLRGIGYRFEVPR"
     gene            3448..6210
                     /locus_tag="VEJ00_00565"
     CDS             3448..6210
                     /locus_tag="VEJ00_00565"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HXW99676.1"
                     /translation="MASKKTKPLWKAEDQGALLTELTASSGDPAKDNPLRRDVRSLGA
                     VLGQVLVEQAGASLFELVEELRRLLIEYRGSSRQRTDRESASALMARAQKIVSEMDLP
                     RAYQVTKAFAIYFELTNLAETNHRKRRRRAGKLDRKHPPLAGSFRGTLLRLKQSGISA
                     EAAMAGLREIEVTPVFTAHPTEVARQTVLLKRRRIAQQLERLDRIPLTDEEAEDAENS
                     IRAEITSLWQTDEVRLAKPSVEDEIRMGLRYLRLSLFAALPRIYAEVAEAFRDVYGVD
                     LDEAALPNLAQFGSWIGGDRDGNPLVKPDCIRDALDTARDLILREYLRDVEALSDRLS
                     TSRRQTAAAEELRERLAYYERTIPGVHLAWGPHNTLESYRRFLSYMFHRLQQSRERAD
                     SAAAYHGAPEFLSDLLLVQSSLQSNRGERLARTYVDSLLRKVRTFGFHLHGLDIRQHA
                     ALHARATEELGPQLNGEAASAETRELLETFRAIAQSKRTHPPQSIRHYIISGAESEDD
                     VLSVIRLARAGGVQLEGSDNDPGLMPVPLFESISSLRAAGPVMRRLWRNPEYEALLDS
                     WGRWQEVMLGYSDSNKDGGMLTSTWELYKAHRDLHRAAQELKVKLRLFHGRGGTVGRG
                     GGPTHAAILAQPPGCFSGHIRVTEQGEVLNWKYSDPVLAEWNLELMLAASLEALLRPA
                     EKKLERTDRWEEAVEEMSQEAYRIYRRRIADNPEVMEYFEQATPVNELDAARIGSRPA
                     RRARGRRLEDLRAIPWVFGWMQSRHAVPAWFGVGHALEQFSKTGPGHERLLRQIARDF
                     PLFSDLIRNVELGMAKADLGIARVYSGLVKNAALGQRVFAMLEDEFLRTRSMILRITG
                     QRELLSRNRVLARSIRLRNPYVDPMSLIQVELLRRKQKGQEGSELEYPLGATINGIAA
                     GLHNTG"
     gene            6468..8579
                     /locus_tag="VEJ00_00570"
     CDS             6468..8579
                     /locus_tag="VEJ00_00570"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HXW99677.1"
                     /translation="MSIFRFSATLSLTLWIAAGLPHNGLAQTGSAEQEGSQLQHFDPN
                     LVDRTLDPCDDFYKYSCNKWMTANPIPPDQVFWGSGSGLQLWNENLLRETLEAAAAAD
                     DPRRSPSQQKIGDYWNACMDERGIEVAGVKPLKPELERIEALKSKNQIALEIARLHQL
                     YTGAWEQSDNQTNAPFFGFTGQQDYDDASKVVGQFDQGGLSLPNRDYYLNTDAKSLEV
                     LKKYRVHVQKMFALAGEPEAQADSDAEIVIELETAMAKAQKDNVWRRDPKNMNNKMSL
                     ADVRKLAPAIDWDIYLKAVNAPASEHYIVTSPDFFRAESKLLEEHPLSQWQTYMRWQV
                     IHRSAPFLNKALVDEDFDFFNHTLAGAEQLPPRWRRCVNAADHDLGEALGQAYVDRAF
                     PPESKARTLQMVHAIEKALDADIHSLDWMSPATKEQAIVKLKGIEDIIGYPSRWRDYS
                     SVKITRDSYLENASQASLFEFERWVAKVGKAVDRTEWGMTPPTINAYYSPQLNTINFP
                     AGILQPPYFDPSMDDAVNYGAIGMIIGHEIIHGFDDEGRKFDAHGNLRDWWTADDGRK
                     YDERGKCISEEYTQDVPEAGPGVRQNGLLTQGEDTADNGGVRLALFALGDTLKQQGKT
                     LDDKGTDGWTYRQRFFLSFANAWCENIRPEIARLAVTTDPHSLSSFRINNVVSNLPEF
                     QQAFGCKAGQRMVRANACRVW"
     gene            complement(8641..9717)
                     /locus_tag="VEJ00_00575"
     CDS             complement(8641..9717)
                     /locus_tag="VEJ00_00575"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HXW99678.1"
                     /translation="MQRFRLFPLLAAVSFLASFCQAQTATTETLMLDLPRASQHAMVL
                     QRIGITDISIKYHRPLANGRQIWGKLVPYGDVWRAGANENTTITFTDPVTVEGQPLDK
                     GTYGLHMIPGETRWTVIFSKNSSAWGSFSYKQEEDALRVNVKPQPADLHDALAYDFDD
                     VKPDSAVVTMRWDKVAVPFKVAVNVNDIVTASIHRQIRGFSQYYWEGWDDAAGYFLAN
                     KINLDEALKDEDQSITIEERYDNLMSKSRILEAMGRGNDAVPVRAKALDLGNAGQLYT
                     YARQLQGEKKQDDAFAIYRSLAKKFPNDWITHAGMARVYSGQGDYENAVKEMKLSLAG
                     APDNNKTIVENYIKRLQAKEDINK"
BASE COUNT         2112 a         2985 c         2998 g         2044 t
ORIGIN      
        1 actgaccatc ccccccagaa tgaccgcacc cactggagtt gaaagcatcc cgcgagtatc
       61 tgagcggggg tcataggttt cttcgtcgag cgtttcccag cggttgccgt gcacatcgaa
      121 gtcgaaggta acgggggaaa gttccacggg cttcccttcg tggtccagtc ctttgaagtt
      181 gtgaacggcg ggggaacctc cggaaaagat cactttgctg ccccttgcgc cggccacaat
      241 gccaaagtgt gctggcccgg gatgtggtgg cagtttggcc cactcgacct tgtcggggtc
      301 cttgtggtcg attttgccgg tccagcactc gtcggattcc acgtagggag cgccgaaagc
      361 ggtgttcttt ttcgcaccat ccacgtagat gatggctccg tcggctaacc cgccggccgc
      421 gccatacact ggcgtgcccg ggaagggagt ggcctggctc caggtattct tctgggcgtc
      481 ataaacctgc acgttgttga ccggtccatt cttggaccgt cccccgacca gataaatgta
      541 acggtcgcga tcgactccga tcaccgcact atcgaccggg accggaatat cctctgctcg
      601 ataccagcgg tgttcgtcgg ccaggtagga attgacgtct ccgacggtaa tttccattcc
      661 ctgcggatcc acgacgtacc ctccaaacac aaatacctgg cctcggactc cgatggcaga
      721 ggcacccagg cggcctgcca cccccgggac gggacgcccc tcagtccatt tcgccccgga
      781 gaggtgaaac acataaacgc ggctggtgac gtcatcccag gttttcttgg ggcctatgcc
      841 catcagggaa taaagctcga ggccgccctt gagggaagcg accgcattgt tggtaaccgc
      901 ggcaggcagg ggaggaaact tgggctgaac ggccgccagc aggacgaccg cgcagagcac
      961 agggaggagg aatacaggga ttttcttcat tgctgaaata ttacgtcttc acgccagcgg
     1021 cggcaaagcg acggcgaggg cttacctggc gaggccctgc cgcaccgcgt gaatatagtc
     1081 ggtataggcc gggattttcc cgttctcgcg catgtgactg gcaatctgcc cacgatggta
     1141 ggccgagtgc atggcgacgt gcgtcaggat atcctcaacc gtgctgtccc acggctcgcc
     1201 cttgctgttc ttgtaggaga tcaagcgcga gagatcctcg gctgacgtct ggtcgaagaa
     1261 ctcgcgccac cgccgcgctt gctccgtgga ttccgcttcg cagcgctcaa aatcgaagtc
     1321 cggccatact ggaaggcttt gcggtttctg cattatgcgc tcgagccaga gacgctcggc
     1381 agcgatgatg tgagccgtca actggagggc acgagggttg tctgagccgc tccccttgat
     1441 tgtggtcagc acttcccggt tggcccacgc gttgtagaca aactggcggc gcagatggtc
     1501 gagaagattc acaaggtcct catactctgc tggtcttcat tggcacgcgt actcgtaagg
     1561 cacggcgtca gtccttcgta ccaggcgctg taaccatttt ttcacaatcc aggactagac
     1621 tgttggtcgg cgccgtatcc ggcagtcccg ggcggcgcca gacaggactt ccagacctca
     1681 atgccgcgca aagcgctgac gacgagccaa acgcccgacg tagaaacgaa cgccgtcgag
     1741 cagccgagcc gaggaactgc tggcaaaacg ccggcggcga ctagagctgg cggccccgat
     1801 gatttagcgc gcctcgcgct gaaagcctgt ctcattgcgc gtgacgcggc gttcaacgtg
     1861 agcgacctgc tgacacattc ttcccggatg gccttcctag ccattaagga ctgcgagaaa
     1921 gaactggacc tgatcgagcg tcagatcgac gaccgcttgc ccgcagccat cacgcgggtc
     1981 aacgaacacc gcgcccggca gttgctttcc agcttgaaga gcacgactga cctggagcga
     2041 atcggggatc ttgtcatgag cgtggccatg cgagtgcagg ccagaactac aaagattccc
     2101 gcttctgacg tgcgccagct ggtggaaatg tcttccgtgc tgcgcgacat gctcgatctg
     2161 attcacaaag gcttctcgac tctcgatctg gagtgcgccc gaaaggtgct ccagatggat
     2221 aaggacatcg accgcgtttg tcatgccctc ttccagaagc acctggcgga gacggttgcc
     2281 cagggtgaca cgtccgggtt tgaggtactg ctgatggctc aggccctcga aagggccggg
     2341 gaccacacta cgaatcttgc cgaagatctc tatagtttga tcgaagggcg cactctgcgg
     2401 catgctccca aaaagaaggc tgcgacctga ggttagaccc agtttgtgct tcgctacaaa
     2461 gtcccgcgac aaattcatcg tgcgttaaca atcgagcaac attcgcgcaa catttcctca
     2521 atacagtctt aatggccttc ccctcttgct tgcaatgcca gtcctgtgac ttcgtggggc
     2581 agcctgaatg cgatgcaact gccagctgaa ttctgcgaaa ccacggacag gagcagcaac
     2641 gtagctgccc ttcgtccagt cgtctttgta gtggaggacg acgaggacat agcccggctg
     2701 atctcccaca atttgcaaca agccgggtac cacgtgcaga gttttgtcag cagtgcttcc
     2761 gtgctgagcg aagccgtgcg ccaggtgccg gcactgtttt tgctggatat catgttgccg
     2821 ggcaccgacg gcttcgaact ctgccgccat attcgccaga cgtcggtgct gtccgccatc
     2881 cccatcatct tcctgaccgc gaagacagca gaagctgacc gggtcaaggg tctggaactg
     2941 ggcggcgacg actacattac caagccgttc agcccccgcg aactggtggc acgcgtccgc
     3001 agcgtgctgc gtggactggg tcagacgccc caggacagcg aagtgatgcg tctcggcgat
     3061 ctgcagattg atatcgcttc catgacggtc acggtgcagg gtcgcaacgt cgtcactaca
     3121 gtgcgcgaat tccgcctgct cgagtacctg gcaacgcatc gaggccgggt tctgacgcgg
     3181 gatcaactgc ttgacgcggt gtggaaggaa acgccttttg taactccgcg gtcgatcgat
     3241 gtttacatcc gccgactccg cgaaaagatc gagtccgacc cccggcggcc ggagtacctg
     3301 aagacgctgc gcggcattgg ctaccggttc gaagtaccca ggtaaggaga cggcaagccg
     3361 tcttctgtac aactccgctg agcgtcatcc actcttttca ttaccggccc gccttgtgtc
     3421 gtttattctg tcgtacatcc ttctgcgatg gcctcaaaga aaaccaaacc gctgtggaaa
     3481 gccgaagatc agggtgcgct cctgacggag ctcacggcga gttcaggcga tcctgccaaa
     3541 gacaatcccc tgcggcgtga tgtgcgttcg ctcggcgcag tgctcggtca ggtgctggtc
     3601 gagcaggcgg gagcaagcct gttcgaattg gtggaagaat tgaggcggct gctgatcgaa
     3661 taccggggat cgtcgcgcca gcgcacggat cgggaatctg cttccgcgct gatggctagg
     3721 gcgcaaaaga ttgtgtccga aatggatcta ccccgcgcct atcaggtcac caaagccttc
     3781 gcgatctact tcgaacttac gaatctggcc gaaacgaatc atcgcaagcg ccggcgccgc
     3841 gcaggaaagc tggaccgcaa gcatcctccc ctcgccggtt catttcgcgg aacgctgctc
     3901 cgcctgaaac agtcagggat ctcggcggaa gcagcaatgg ccgggcttcg cgagattgag
     3961 gtgacgccgg tttttacggc acatcccacc gaagtggcgc gccagactgt cttgctgaag
     4021 cggcgccgca tagcccagca actggagcgt cttgaccgca ttccgctgac ggatgaggaa
     4081 gcagaagacg ccgagaacag cattcgagcg gaaatcacct ctttgtggca gacggacgaa
     4141 gtccggctgg ccaagccttc ggtagaggat gaaatccgca tgggcctgcg gtatttacgc
     4201 ctctcgcttt tcgccgcgct tcccaggata tacgccgagg ttgcggaagc cttccgcgac
     4261 gtttacggcg tcgacctcga cgaggctgct ctccccaacc tcgcgcagtt cggctcctgg
     4321 attggcggcg accgcgacgg caacccgctg gtcaagccag actgcatccg cgatgcactg
     4381 gatacggcgc gggatctcat cctccgggaa tatctccgcg atgtggaggc gttgagcgac
     4441 cggctcagta cttcccgacg acagactgca gctgcggagg agctgcggga gcgactggcc
     4501 tactacgaac gcacgatccc gggtgtgcat ctggcgtggg gaccgcacaa tacccttgag
     4561 agctatcgcc gttttctctc gtacatgttc cacaggctgc agcaaagccg cgaacgggca
     4621 gattcagccg cggcctatca cggtgctccg gaattcctga gcgatcttct gctggtacag
     4681 agcagcctcc aatccaaccg cggcgaacgg ttggccagga cctacgttga ctcgctgtta
     4741 cgcaaggtcc gcacgttcgg ttttcatctg catggtttgg atattcgcca gcatgctgcg
     4801 ctgcatgcgc gtgcgacgga ggaacttggc cctcagctca atggcgaagc cgcgtcggcc
     4861 gagacgcgcg aactgctcga aacctttcgt gcgatcgcgc aatcaaagcg cacgcaccct
     4921 ccgcagtcga ttcgccatta catcatcagt ggtgctgaat cagaagatga tgtgctcagc
     4981 gtaattcgcc tggcgcgagc aggcggggtt cagctggagg gatctgacaa tgacccgggg
     5041 ctcatgccgg tgcccttgtt cgaatcgatc tcgtcgctgc gagctgcagg gcctgtcatg
     5101 cggcgtttgt ggcgcaatcc cgaatacgaa gccctcctcg attcctgggg acgctggcag
     5161 gaggtcatgc tgggctactc cgactccaac aaagatggtg gaatgctgac aagcacctgg
     5221 gaactttaca aggcgcatcg cgacctgcat cgcgcagcgc aggaattgaa agtgaagctg
     5281 cgactgtttc atggccgggg aggaaccgtc ggccggggag ggggaccgac tcacgccgcg
     5341 attctcgccc aaccgcccgg ctgcttctcc gggcatattc gcgtcaccga acagggtgaa
     5401 gttctcaact ggaaatactc cgatccagtc cttgcggaat ggaatctgga actgatgctc
     5461 gcggccagcc tggaggcgct cctgaggccc gccgaaaaga agttggagcg caccgaccgg
     5521 tgggaagagg ccgtggaaga aatgtcgcag gaggcgtacc gcatttatcg ccgccggatc
     5581 gccgacaatc ctgaagtgat ggaatatttt gagcaggcaa ctccggtgaa cgaactcgac
     5641 gcggcccgaa tcggatcccg tcctgcacgc cgggccagag gccgccggct cgaagatctg
     5701 cgcgccatcc cctgggtttt tggctggatg cagagccgcc acgccgtacc tgcctggttt
     5761 ggcgtcggac acgcgctgga gcagttttcg aaaaccgggc cgggtcacga gcggctcttg
     5821 cgccagatcg cccgtgactt cccgcttttc tcagatctga tccgcaatgt ggaattggga
     5881 atggcgaagg ctgatctggg aatcgcgcgc gtttattcgg gactggtcaa gaatgcggcg
     5941 ctgggccagc gagtcttcgc catgctggaa gacgagttcc tgcggacacg cagcatgatt
     6001 ctccgcatca ccggacagcg cgaactgctc agccgcaatc gcgtgctggc tcgatcgatc
     6061 cgcctgcgta atccctatgt cgatcccatg agcctgattc aggtagaact gttgcgccgc
     6121 aagcagaagg gtcaggaagg atctgagctg gaatatccct tgggcgcgac gatcaatggg
     6181 atagccgccg gcctgcacaa tacaggctaa cgaagtttga ggatgaacgc aggttgctgc
     6241 gagatggcgc caggaatcaa gaaccggcga atctgcgcag gccagtccag ccgctaacgt
     6301 cgcgtctgcc aagccgtctg atattctgct cgtttcgtgc tgaagtgcat tctacaagcc
     6361 cctcggtacg gctgggttcc tgttctaatg cacacgttct atcgaacagg tcaccttatc
     6421 tgactcgtga cttccacatt tcgatgactt ctatcaaaag agggttcatg tccatcttcc
     6481 gtttctcagc taccctgagc ttaactttat ggatcgcggc aggcttacct cataacggac
     6541 tggcgcagac cggatcggca gagcaggaag gatcccagtt gcagcatttc gatccgaatc
     6601 tggtcgacag aactctggac ccctgcgatg acttctacaa gtactcctgc aacaagtgga
     6661 tgacggccaa tccgatcccc cccgaccagg tattttgggg cagtggcagc ggcctgcagt
     6721 tgtggaatga gaatctgctc cgtgaaacgc tcgaagcggc cgccgccgcg gacgatccaa
     6781 ggcgtagtcc cagccagcag aagatcggtg actactggaa tgcatgcatg gacgagaggg
     6841 gcatcgaagt cgcaggtgtc aaaccgctga aaccggaact ggagcgcatc gaagctctca
     6901 aatccaaaaa ccagattgct cttgagatcg cgcgcttgca ccagctgtac accggagcgt
     6961 gggagcagtc tgacaaccag acgaacgccc cgtttttcgg cttcaccggg cagcaagatt
     7021 atgacgacgc atcgaaggtg gtggggcagt tcgatcaggg tggtctgagc cttcccaatc
     7081 gcgactacta tctgaacacg gacgccaagt ccctcgaagt gctcaagaag taccgcgtgc
     7141 acgtacagaa gatgtttgcg ttagcggggg aaccagaagc acaggccgac agcgacgccg
     7201 aaatcgtgat tgagctcgaa accgccatgg ccaaggcgca gaaggacaat gtctggcgtc
     7261 gcgatcccaa gaacatgaac aacaagatga gcctggccga cgtccgcaag ctcgcgcctg
     7321 ccatcgactg ggatatctac ctcaaggcgg taaatgcgcc agccagcgag cactatatcg
     7381 tcacgtcgcc agacttcttc cgtgcggaga gcaagctgct tgaggaacat ccgctgagcc
     7441 agtggcaaac atacatgcgc tggcaggtga ttcatcgctc ggcgccattt ctgaataagg
     7501 ccctggtgga tgaagacttt gatttcttca accacaccct ggcaggcgcc gagcaactgc
     7561 cgccgcgctg gcggcgctgt gtgaacgccg cggaccatga cctgggcgag gccctcggcc
     7621 aggcctacgt cgaccgcgcg ttcccgcccg agagcaaggc tcgcacactg cagatggtcc
     7681 acgcgatcga aaaggcgctg gacgcggaca ttcacagctt ggactggatg tcgccggcca
     7741 ccaaggaaca ggcaatcgtc aaactgaagg ggatcgaaga catcatcggg tatccctcgc
     7801 gctggcgtga ctattcctca gtcaaaatca cccgcgacag ctatctggaa aacgcgtcgc
     7861 aggcttcgtt gttcgagttc gagcgttggg tggcgaaggt cggcaaggcg gtggaccgca
     7921 ccgagtgggg aatgactccg ccgaccatca acgcctatta ctcaccgcaa ctcaacacca
     7981 tcaatttccc cgccggcatt ctgcagccgc cgtacttcga tccctccatg gacgacgcgg
     8041 tgaactatgg cgccatcggc atgatcattg ggcacgagat catacatggc tttgacgacg
     8101 aaggccgcaa gttcgacgct cacggcaacc tgcgcgattg gtggacagcg gacgacggca
     8161 gaaagtacga cgaacgcggc aagtgcatct ccgaggaata cacccaggac gtgcccgagg
     8221 cgggaccggg ggtgcggcaa aacggactcc tgacgcaggg agaagatacc gctgacaatg
     8281 gcggagttcg gctcgccctg ttcgcgctcg gagacaccct gaagcagcaa ggcaagacgc
     8341 tcgatgacaa agggacggac ggatggacct atcgccagcg gttctttctg tccttcgcca
     8401 acgcctggtg tgagaacata cgtccggaga tcgcgcgcct ggccgtgacc acggacccgc
     8461 attccctgtc cagtttccgc atcaacaatg tggtttcgaa cctgccagaa ttccagcagg
     8521 ccttcggatg caaagccgga cagcgcatgg tacgggccaa tgcttgccgc gtctggtagg
     8581 tagtttcgcg ggggataaga agggccagca gatggccctt ctttcggcag catgaggcgc
     8641 ttacttgttg atatcctctt tggcctgcag gcgcttgatg tagttctcca caatggtttt
     8701 gttgttgtct ggcgcacccg ccagcgaaag cttcatttcc ttcaccgcgt tctcgtaatc
     8761 gccctggccg ctgtagacac gcgccattcc cgcatgcgta atccagtcgt tgggaaattt
     8821 cttggccagc gaacggtaaa tggcgaacgc gtcgtcctgc tttttttcgc cctgtagctg
     8881 tcgtgcatac gtgtaaagct gccccgcatt tcccaagtcc agcgctttgg cccggaccgg
     8941 cacagcgtcg ttgccgcggc ccatcgcctc aagaatgcgc gacttgctca tcaggttgtc
     9001 gtaccgctcc tcaattgtta ttgactgatc ttcatctttc agcgcctcgt caagattgat
     9061 cttgttggcg aggaagtagc cagccgcgtc gtcccatccc tcccagtagt actggctgaa
     9121 gccgcgaatc tgccgatgaa tgctggcggt aacgatgtcg ttgacgttga cggctacttt
     9181 gaagggaacg gcgactttat cccagcgcat cgtgacgact gcagaatccg gcttgacgtc
     9241 gtcgaagtcg taggctagcg cgtcgtggag gtcagccggc tgcggcttga cgtttacgcg
     9301 cagcgcgtcc tcttcctgct tgtagctaaa gctgccccag gcgctggaat ttttcgagaa
     9361 gatcacggtc caccgggtct cgcccggaat catgtgcagc ccgtaggttc ctttgtccag
     9421 cggctggcct tcgacggtga caggatcggt aaacgtaatg gtcgtgttct cgttcgctcc
     9481 cgcccgccag acgtcgccgt aaggcacgag tttgccccag atctggcggc cgttggctaa
     9541 aggccggtga tatttgatgc tgatgtcggt gattccaatg cgctgcagca ccatggcatg
     9601 ctggctcgca cgtggcagat cgagcatcag ggtttcagtg gtggcggttt gcgcttggca
     9661 gaaactggca aggaacgaaa ctgccgcaag caggggaaac agtcgaaatc gttgcatgga
     9721 agctccttgg cgatgctcgg atcgagcgct gtttgggaat ggagagatga ttgggcacgc
     9781 gcaccccagc gctgtgccac gttagcctta gacgcttgtc agaaacggaa gtaagcactg
     9841 cttgacgcgt gttcagaaac ggacgccgaa tcccgatgga taggaatgcg gcagtctgca
     9901 aatcttgcat tcgaaaattc tgacttgaca aatcagcccc caaagcacag aatttcttta
     9961 ggcgaacaaa aagcgaaaac gcctccgacg gcacgcttat ccagagcacc agccccggcg
    10021 ttccctgaaa tcattccgag gtatctatgt cttccgcggt ggtttccact tcggcggccc
    10081 tgagccgcct tttcgtcttt gatgaagaaa cagagcttga ggcctgctcg agcgtaaac
//
LOCUS       DASPUL010000026         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1375161, whole genome shotgun sequence.
ACCESSION   DASPUL010000026 DASPUL010000000
VERSION     DASPUL010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1375161"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VEJ00_00580"
     CDS             <1..206
                     /locus_tag="VEJ00_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99679.1"
                     /translation="AEAFSPFPLLPERLKALTNEIAEILPKRSGAIVLTLEFNQFQEH
                     LRGLEPPKPEPKKPMLLRDSPPI"
     gene            420..1265
                     /locus_tag="VEJ00_00585"
     CDS             420..1265
                     /locus_tag="VEJ00_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99680.1"
                     /translation="MAVKKLAPSKVNTRQSVALRSDPTRIDPRKLKLGKHPARRDPRT
                     LLLASYITGALPAPPASGDLTTKVGSAWGMMDNDQIGDCTCAAAGHLIMEWTANASKK
                     MVTPSDKQIVAAYSAITGYNPTTGANDNGAVEIDVLNYWRQSGIAGHKIGAYAALEPS
                     NHTNVMDAVYIFEGCYIGLQLPTSAQAQVQNHQPWSVPPGGTSGDGAPGSWGGHAVPV
                     VAYDARTLTVVTWGALQVMTWSFWDAYCDESYAIISNDYINNKKESPDGFNMQQLQAD
                     LADLK"
     gene            complement(1335..2042)
                     /locus_tag="VEJ00_00590"
     CDS             complement(1335..2042)
                     /locus_tag="VEJ00_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99681.1"
                     /translation="MRLPILVIHIIGGTLGMLSGYVAIFLRKGSRRHAIAGKVFVVSM
                     LIMAACGVVLAALKSDPSNMIGGTFTFYLVGTAWLAGRRKDREAGTSLLDWAGLLVTL
                     GVSALEITWGSQAALSPTGMKFNYPPGPFFFMGSVAVLATVGDVRLLLRGGIAGTQRV
                     ARHLWRMSFGLFIAAASIFLARAHLFPRFMRKTGMLAVLSFLPLALLVFWQIRVRLVS
                     AYKERRQSRASLIHSPA"
BASE COUNT          475 a          748 c          603 g          368 t
ORIGIN      
        1 tcgccgaggc attcagccca tttccactgt tgcccgagcg cctcaaggct ctcacaaacg
       61 aaatcgccga aatcctgccc aagcgcagtg gggcgatcgt tcttaccctc gaattcaacc
      121 aattccagga gcaccttcgc ggtctggagc cacccaagcc agaaccgaaa aaaccaatgc
      181 tgctgcgaga ttcgccgccc atctgaagga ccaatttgaa gtataggtcg atcgatccaa
      241 tattccctgt ccggttttcg taacaacttc ctcttccccg ttgacgcagc cgccgtctcg
      301 gtctaagatt ccggcctcct tccccccaag cccgatccgc gcgcgcagct gaggctggtg
      361 cgcggagggc ctctttcgcc atggtctaaa tttctggtgg ctcccaagga gactgctcta
      421 tggccgtgaa aaaactcgca ccgtccaaag tcaatacccg ccaaagcgtc gccctgcgct
      481 ccgatcccac gcgcatcgat cctcgcaaac tcaaactcgg gaagcatccg gcgcggcgcg
      541 atccgcgcac gctgctgctc gcctcgtaca tcaccggcgc cctacctgcg ccgcccgcaa
      601 gcggcgatct caccaccaaa gtcggctccg cgtgggggat gatggacaac gaccagatcg
      661 gcgactgcac ctgcgccgcc gccggccacc tcatcatgga gtggaccgcc aacgccagca
      721 agaaaatggt cacgccctcc gacaagcaga tcgtcgccgc ctactcggcc atcaccggct
      781 acaacccgac caccggcgcc aacgacaatg gcgccgtcga aatcgatgtg ctcaactact
      841 ggcgccagtc tggcatcgcc ggccacaaga tcggcgccta cgcggcgctc gagccttcca
      901 accacaccaa cgtcatggac gccgtctaca tcttcgaggg ttgctacatc ggcctgcagt
      961 tgcccaccag cgcgcaggcc caggtgcaga atcatcagcc gtggtcggtg ccgccgggcg
     1021 gcaccagcgg cgacggcgct cccggctcct ggggaggcca cgccgttccc gtcgtcgcct
     1081 acgacgcgcg cacgctcacc gtcgtgacgt ggggagcgct gcaggtgatg acctggtcgt
     1141 tctgggacgc ctactgcgac gagtcctatg cgatcatcag caacgattac atcaacaaca
     1201 agaaggaatc gcccgacgga ttcaacatgc agcagctgca ggccgatctc gccgacttga
     1261 agtgagcgga tatgggcgcg ctcaggactg tggcccacca cctctaaata ttttcccggg
     1321 tgggtgtttc ttcttcacgc gggtgaatga atcaggctcg cacgcgactg gcggcgttcc
     1381 ttgtaggcgc tgaccaggcg aacccggatc tgccagaaga ccagcagcgc cagcggcagg
     1441 aaactgagca cagccaacat gccggtcttg cgcatgaacc tggggaacaa gtgagcccgc
     1501 gccagaaaga tcgacgcggc cgcgatgaac agcccgaagc tcatgcgcca gagatgccgc
     1561 gccacccgct gtgtacccgc tatgccgccg cgcaacagca gtcgcacatc accgaccgtc
     1621 gccagcaccg ccactgatcc cataaaaaag aaaggtccgg gtggatagtt gaatttcatt
     1681 cccgttgggc tcagcgcggc ctgcgatccc caggtgatct cgagggcaga gactcccagc
     1741 gtcaccagaa gcccggccca gtcgagcagg ctggtccccg cctcgcggtc tttgcgccgt
     1801 ccggccagcc atgcagttcc tacaaggtag aaggtgaacg tgccgccgat catgttgctc
     1861 gggtccgatt tcagggctgc cagaaccaca ccgcacgccg ccatgatcag catcgacacg
     1921 acaaacacct ttcccgctat agcatgccgc cgcgagcctt tgcgcaggaa aatcgccacg
     1981 tatcccgaca gcattcccag cgtgccgccg atgatgtgaa tcacgagaat gggcaatcgc
     2041 atggcttcgc actccttggc tcacttcgac agtacgtaga agagagcggt gacaccgatg
     2101 tagaagacgc tcacgatcag atcgacggcg agcgccaccc tgccgtgaaa aaagagaacg
     2161 aatgcgccgg tcagaatcgt gagcagatag taga
//
LOCUS       DASPUL010000027         4067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1512038, whole genome shotgun sequence.
ACCESSION   DASPUL010000027 DASPUL010000000
VERSION     DASPUL010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512038"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..1657)
                     /locus_tag="VEJ00_00595"
     CDS             complement(245..1657)
                     /locus_tag="VEJ00_00595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXW99682.1"
                     /translation="MRNLLREKARFFLGAGLAALALRLVFVLRFPGVVDDSRLYANIA
                     ENWLRHGIYGITDSGQIIPTLSRLPGYPAFLAAIFSIFGIGNFRAVLLVQVVFDLATC
                     LLIADLARRFFGARAAQAAFLLAGLCPFLADYAAAALTETLEIFFTALALDLACRGLG
                     VGADRPIQWPHLVWLGCGLAVGAAILLRPDGGILLATIAGYLIYRLARSVWAGQSTSP
                     VDRRAWLGAIVLVIVGVAVPLIPWTLRNLHTLHRFEPLAPRYANDSDELVMKGFNRWV
                     KTWIADYVSVQEIYWNVPGDAIDVSRLPRRAFDSEQQRKVTAELFADYNVDHDMTPEL
                     DARFAALAAERIQAAPVRYYVRLPALRVADMWLRPRTELLASDPRWWEFNDEAAWLAV
                     SIAFGVINLLYVMLAGIGLFRGGGMTGVGLLILFVVLRSAFLGTLENPEPRYTLECYP
                     ALIVVASACFVELSQRQMFR"
     gene            1785..>4067
                     /locus_tag="VEJ00_00600"
     CDS             1785..>4067
                     /locus_tag="VEJ00_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXW99683.1"
                     /translation="MTQPVFYDPRRARWKRVRRLFDVVAVVVSALIIFFVFTALRDEP
                     LPELLFSPQKRAFKALKESEKEKARDRQKKLAARSHRKSKLAASQVKLNQEEGIRAAF
                     YVPWDAASFSSLRSYARQIDILYPDWLHVLTADGRLQGVDDQTNKFFDVVQGATVRSV
                     DDRVMPFLKLEDPGMEVFPMVNNFDNADWVDISSFLNNPDSRSLFRGQVSQFLSSGHF
                     RGLMIDFEAFPAAGQPGYVALLNELASDLHARGLKLYVAVPPHNGEYDYPAVAAAADG
                     VVLMNYDEHYPGAASGPVASQDWFVQNLTFARTVIPKDKIICAIANYGYDWVLKPKKG
                     KLPPSIHDTTVSVQEAWLAARDSDEDVNFDDDAMNPHFSYLDEQSLRHDIWFLDAVTA
                     LNHIRAAQGLGIQTFALWRLGSEDRSLWKVWDVPGDPGAADKLKDVPPGADVDMEGQG
                     EILRIEDKPAHGIRDLTIDADTKQITDEVFQNLPEPYRVGRYGYSPNKIALTFDDGPD
                     PEWTPKILDVLRDKHATATFFLIGIQTDKFSGIAKRVYREGNTIGNHTFTHPDVSNIS
                     TTYMKVELNLTERLFASLVGVRATLMRPPYAIDEEPDTADQVRPLEIPQEMGYITVGN
                     RIDPNDWSSNPRRSAEQITSYVLSHLPPCRVDDLRCGNIVLLHDGGGDRSETVRALPM
                     IIDGIRARDYEIAPVYDLIGKTRADVMAPLPTGELWAARLDSLGFWIFDAGVVGITWI
                     FLLGDLLMTGRLIFIGAAAVY"
BASE COUNT          864 a         1327 c         1127 g          749 t
ORIGIN      
        1 gcataggagc tcctctaccc acgggggcat tacaattttc aattgtgttt cgtcgaggac
       61 ctggaagaca gatgagaccc tgagacccga cgtcgggcta ttgtgcaact acgagaccat
      121 atctgcaagc cttaattttc aatgggttgc atcaagatcg acaatcctcg cagaggttgc
      181 catacacctt tccagagatg acgtcccagt ttttggaaat tgacatgctg tatgccgatg
      241 tttgttatct aaacatctgg cgctgcgaaa gttcaacgaa acaggctgaa gctacaacaa
      301 tcagggctgg ataacactcg agcgtatagc gcggctccgg attttccagt gttcccagaa
      361 acgcggagcg caaaacaacg aacaggatca gcagccccac gcccgtcatg cctccacctc
      421 gaaacagtcc aattccggcg agcatgacgt acagcaggtt gatcacgcca aaggcaatgc
      481 tgactgcgag ccatgccgct tcgtcgttga actcccacca gcgcggatcc gaggccagaa
      541 gctcggtccg gggccgcagc cacatgtcgg cgacgcgcag cgcgggcagc cgcacgtaat
      601 agcgaactgg cgctgcctgg atgcgctccg cggccagtgc tgcgaagcgc gcgtcgagtt
      661 ccggggtcat atcgtggtcg acgttatagt cagcgaacag ttcggccgtc actttccgct
      721 gctgctcgga atcgaaggcc cggcggggca agcgcgaaac gtcaatcgca tcgccgggca
      781 cgttccagta gatctcctgt accgaaacat agtcggcgat ccatgtcttc acccagcggt
      841 tgaagccctt catcaccagt tcgtcggaat cgttcgcata ccggggagcc agaggttcaa
      901 accggtgcag cgtatgcaga ttgcgtagag tccagggaat cagcggaacc gcaacgccca
      961 cgatgaccaa aacgatcgcg ccgagccagg cacgcctatc gaccggcgac gtgctctgcc
     1021 cagcccacac ggagcgcgcc agccgataga tcaggtatcc cgcgatcgtt gcgagcagaa
     1081 tcccgccatc cggtcgcagc aggatggccg ccccgaccgc aagcccgcat ccgagccaaa
     1141 ccaggtgcgg ccactgaatg ggccgatccg cgccaacccc gagcccgcgg caagccagat
     1201 cgagagcgag cgccgtgaaa aagatctcca gggtctcggt gagggctgcg gctgcgtagt
     1261 cggccagaaa aggacacagc ccggccagca gaaacgccgc ctgcgccgcc cgagctccga
     1321 agaaacgccg cgccagatcc gcgatcaaca agcacgtggc cagatcgaaa accacctgca
     1381 cgagcaaaac ggctcgaaaa ttgccaatac cgaagatcga gaagatcgcc gccagaaacg
     1441 caggatatcc gggcagccgg gagagcgtcg gaatgatctg cccggagtcg gtgatgccgt
     1501 agatgccgtg ccgcagccag ttttctgcga tgttggcata caggcgggaa tcgtccacaa
     1561 ccccgggaaa acgcaggaca aacaccagcc gcagcgccag tgccgccagg cccgccccaa
     1621 gaaagaaccg cgccttctcc cgcagcaggt ttcgcacgca gcgaatcata gcgcgtttgc
     1681 cggggacccg cgcgttggaa cgccttcaga gcgttactca ctcagcccca ccggagcatt
     1741 gaccctgctc cccccgcggc ttacgatgag aatgccgttt ccccatgaca caacccgtct
     1801 tttatgatcc ccgacgcgcg cgctggaagc gcgtacggcg actgttcgat gtcgtggctg
     1861 ttgtcgtctc ggcgctgatc attttctttg tcttcaccgc cctgcgcgat gagcccctgc
     1921 cggaattgct cttctctccc cagaagcgtg ccttcaaggc cctgaaggaa agcgagaagg
     1981 agaaggctcg cgaccgccag aagaaacttg ccgctcgctc tcatcgcaaa tccaagctgg
     2041 ctgcctcgca ggtcaagctg aatcaggaag aaggcatccg cgccgccttc tacgtgcctt
     2101 gggacgccgc cagtttctcc tcgctgcgca gttacgcccg ccagatcgac atcctttatc
     2161 ccgactggct ccacgtgctg actgctgacg gacgcttgca gggtgtcgat gaccagacca
     2221 acaagttttt cgacgttgtc cagggagcca ccgttcgctc cgtcgacgac cgcgtcatgc
     2281 ccttcctcaa actggaagac cccggcatgg aagtcttccc catggtcaac aactttgaca
     2341 atgcggactg ggtcgacatc tcctcctttc tgaacaaccc ggattctcgc tcgcttttcc
     2401 gtggccaggt ttcgcagttt ctctcgtcag gtcactttcg cggcctgatg atagatttcg
     2461 aagctttccc tgccgccggc cagcctgggt acgtcgcgct gctgaacgaa ctcgcgtctg
     2521 atctgcacgc ccgcggcctg aaactctacg tcgccgtgcc gccgcacaac ggcgagtatg
     2581 actacccggc ggtcgcggcc gccgccgacg gcgtcgtgct catgaactac gacgagcact
     2641 atcccggtgc cgcctccggc ccggtcgcct cgcaggactg gtttgtgcag aacctgacct
     2701 tcgcccggac cgtgatcccc aaagacaaga tcatctgcgc tattgccaac tacggctacg
     2761 actgggtgct caagccaaaa aaaggaaaat tacctcccag cattcacgac acaaccgtca
     2821 gtgtgcagga ggcatggctg gccgcgcgtg attctgacga agacgtcaat tttgacgacg
     2881 atgccatgaa cccgcacttt tcctatctcg acgagcagag cctgcgtcac gatatctggt
     2941 tcctcgatgc cgtcacggcc ctgaatcaca tacgcgctgc gcaggggctg ggcatccaga
     3001 cgtttgcctt gtggcggctc ggcagcgagg accgttcgct ttggaaggtg tgggatgtgc
     3061 ccggcgatcc cggggcggcc gacaaactaa aggacgttcc gcccggcgcc gacgttgaca
     3121 tggaaggcca gggcgagatc ctccgcatcg aagacaagcc cgcccacggc attcgcgact
     3181 tgaccatcga cgccgacacc aagcagatca ccgacgaagt cttccagaat ctccccgagc
     3241 cttaccgcgt cggccgctat ggctacagcc cgaacaaaat tgcgctcacc ttcgacgacg
     3301 gccccgaccc cgagtggaca ccaaagattc tcgacgtgct tcgagacaag cacgccacgg
     3361 cgaccttctt cctgatcggc attcaaaccg acaaattctc gggaatcgcg aaacgcgttt
     3421 atcgcgaagg caacaccatc ggcaatcaca ctttcacgca tcccgacgtg agtaacatat
     3481 cgaccaccta catgaaggtg gaactgaatc tcaccgagcg cttgttcgcg agtttggtcg
     3541 gagtccgcgc tacgctgatg cggccgccct acgccatcga cgaggaaccc gataccgccg
     3601 accaggttcg cccgctcgag attcctcagg agatgggcta catcaccgtc ggcaaccgca
     3661 tcgatcccaa tgactggagc agcaatcccc ggcgttccgc cgaacagatt acttcttacg
     3721 tgctgtcaca cttgccgccc tgccgcgtgg atgatctgcg ctgcggcaac atcgtgctct
     3781 tgcacgatgg cggcggcgac cgctctgaaa ccgtgcgcgc cttgcctatg atcatcgacg
     3841 gcatccgcgc tcgcgactat gagatcgctc ccgtctacga tctgatcggc aagacgcgag
     3901 ccgacgtaat ggcgcccttg ccaacgggcg aactctgggc ggcgcggctc gacagtctcg
     3961 gtttctggat tttcgacgcc ggcgtggtgg gcatcacctg gatctttttg ctcggcgacc
     4021 tgctcatgac cggccgcctg attttcatcg gagccgccgc cgtctat
//
LOCUS       DASPUL010000028         8595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1923503, whole genome shotgun sequence.
ACCESSION   DASPUL010000028 DASPUL010000000
VERSION     DASPUL010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8595)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8595
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1923503"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1285
                     /locus_tag="VEJ00_00605"
     CDS             92..1285
                     /locus_tag="VEJ00_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HXW99684.1"
                     /translation="MLRKNRLFTPGPTPLLPAAQTAMAAFTAHHRTADFRALFQRVLG
                     DMKEFIGTKNDVLVLACSGTGVMEAAVTNLTSPGDKVLVLTAGKFGERWTGLAKAFGC
                     NVDVFSVPYGETFSLDDVRARVKPDVRAVFVQATESSTGARHDVQGIAKIVRGQGDQT
                     LLVVDAITGLGTTHLDVDGWGVDVTIGGSQKALMMPPGLAYCAVSERAWKRMDSTTSP
                     RYYFDLRKERKSAAKGETAYTPATSLFAAMGAALEFVRGMGNGDLAKGREELVNNAEL
                     CAEMTRAGAKALGLKLYASAPAAALTAISSPDGLDSGKIVKEFRESFDAVVANGQGEM
                     KGKIFRIAHIGYYDYLDTVGILGALEHVLAKTAGRSVEYGAAVKAAQEVYARSLAGTR
                     QLVGA"
     gene            complement(1328..1732)
                     /locus_tag="VEJ00_00610"
     CDS             complement(1328..1732)
                     /locus_tag="VEJ00_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HXW99685.1"
                     /translation="MKAVNTLKTILLAAGIALMMACGYSHSMTPPTAGTMPVIKTLNP
                     TDVNHGTAAFSLEVDGTGFNSTAVVNFNQAAMATTWTNSGKVTAMIPASAIMNAGMVN
                     VTVTNPAVTGTGPYGGGGMTAQTSTPIAFTIN"
     gene            1905..3506
                     /gene="serA"
                     /locus_tag="VEJ00_00615"
     CDS             1905..3506
                     /gene="serA"
                     /locus_tag="VEJ00_00615"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXW99686.1"
                     /translation="MKIVVAEKISPSAVAELQEPGWTVLTADQLDGKLAEHLESADAL
                     IVRSAVQADAALLQHAKKLRVIGRAGVGVDNIDLEAATHKGIAVMNTPGANAVAVAEQ
                     TLGMMLAMARHLCRADALMHSGKWEKKSLQGTELRGKTLGIVGLGRIGMEVARRAKAF
                     GMKLVAHDPFVSVAVAKDQEIRLAGLDELYAAADYITLHVGLTPQTAGMINRDSIAKM
                     KKGVRLVNCARGELVNETDLAAALKSGHIAAAALDVFADEPLKNSPLQALENVVLTPH
                     VGGSTFEAQEAVGVQIAQQVKEYLKHGVIQNAVNVPSVSAEEYAAMQPYIVLAERMGA
                     FLAQVSEGTIEEISIRYSGHIAEGKTELIRNGAIKGILNQALEEKANLVNAATIADAR
                     GLRVLEVRKEKASIGGAGSVISIFLKSSTEEHLVKGAVLHETAPRLLHVDGIDVEAPL
                     ERNLIYLRNRDVPGVIGKVGTILGAESINIADFSLGRRSAEKDSAQPREAIAVVHVDG
                     HVPDRVLFKLRQIPAVQKAKAVRLF"
     gene            complement(3598..4722)
                     /locus_tag="VEJ00_00620"
     CDS             complement(3598..4722)
                     /locus_tag="VEJ00_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXW99687.1"
                     /translation="MRSWSIPIGRLFGVDVRLHLTFFLLPLFIFWTDYAAHQGDANGS
                     RDLILTGIILACVAAHESGHMLAARRFGLIPKAVILLPLTGVTLYDESRSVKQPSTLA
                     WKREIRLTVVGPLVSLALASLAAAIIAASGRNIELFKWPFLTARNLPRSVVWANLYLA
                     LLNFMPAYPLDGGRILRAFFSRTLDASAASRRAVSISNAIAMVLMVAGLFSDNWLTMV
                     GLIVFSAAQLEERALVFQSVLDNVRLEEVMLTDFATLSPADTLEDALEKAVHSLQDDF
                     PVVRGSDMVGVISRQRILDALRAEGNGYVQAVMNKIFDVSVRQDSLGSAFRKLTSRSA
                     SIIPVVEDQRLVGIVTLQNLMHSMSLLAESRKLRRDETEQ"
     gene            4789..5376
                     /gene="rsmD"
                     /locus_tag="VEJ00_00625"
     CDS             4789..5376
                     /gene="rsmD"
                     /locus_tag="VEJ00_00625"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HXW99688.1"
                     /translation="MRVIAGKYRSRPLRSKRGMDIRPTSDRLRETLFNVLTAGNPAAL
                     EGSVWLDLYAGTGAVGIEALSRGARQVYFVESSAEAAEVIRANLKSLNISSGFKLLHD
                     ELPRAFWRMEREHVAADVVFVDPPYRMKEAYRDTLRALAESPVIWAMSMVIAEHEKKF
                     DPGEEFGPLRRFRKLVQGSVALSFYRRASAADVRT"
     gene            complement(5433..5984)
                     /locus_tag="VEJ00_00630"
     CDS             complement(5433..5984)
                     /locus_tag="VEJ00_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99689.1"
                     /translation="MIPESHPLQQLFQDLVGRHYAGEIGIRDPQIVAYVSHLLAEFCE
                     ADQLYKIRDAANRPLADVGEMLLESDPVYGPAPSFDRERQVRKHIGDYTLFFAGMFPE
                     SINHFRLRRHRLENLVEWIKAGKESYYIVSKFEHFEYAKVAPLFATLSRNFEGCVFGL
                     NMVKNELAVMQHPIARAPDDLMM"
     gene            6265..6663
                     /locus_tag="VEJ00_00635"
     CDS             6265..6663
                     /locus_tag="VEJ00_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXW99690.1"
                     /translation="MERQNISSGAPWEPVVGYSRAVKVGQYVHVSGSTAIGADGKIVG
                     VGDVYAQAVQTLKNIESALKKAGASMKDVVRTRMYVLNIAEWEKVGKAHGEFFRDVRP
                     ATTMVQVSALISPDMLVEIEADAVLPGTRA"
     gene            complement(6664..7131)
                     /locus_tag="VEJ00_00640"
     CDS             complement(6664..7131)
                     /locus_tag="VEJ00_00640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXW99691.1"
                     /translation="MQERVAEVKDAPAIIAVINAAFRRAESFLIDRDRVDLEMVRSLL
                     ERGKFLVAEDGGVLAGCVYIELRGDRAYLGLLSVDPDRQKAGLGSQLMNAAENYCARA
                     GCRFMDLRIVNVRRELPAFYRHRGYVETGTAPFTPGLNPKVPCHFVEMSKPLS"
     gene            7264..8571
                     /locus_tag="VEJ00_00645"
     CDS             7264..8571
                     /locus_tag="VEJ00_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXW99692.1"
                     /translation="MKISRCLFVLALAVPVIAQQTRPAAGQQVTVIRAGTLIDGKSQQ
                     PKRDQVIVVRGNSIESVGDAASAKIPTGATVIDLSRQTVLPGLIDSHTHIFLWGEDPE
                     KGGYDVNILMAPFGLRAARATVAVRRALEQGFTTLRDVETEGAGYGDIGIKQAIEDGI
                     IPGPRLFVATRAISTTGGYMLEGYAPELVMPKGAQIADGPVEARKAAREQLDHGADWI
                     KVYMTHRSWVGKNGELVSQPTFTVDELKAIVDETHGWGKKVACHAYGGIGLHRALDGG
                     CDSIEHGLDLDDAAIAQMLKQGTWYVPTLSPYYYDWAPADTPNGKRDRLRAAVHEPSF
                     KKALKAGVKIVFGTDMGGIPWTDPIAQEFSRMVSFGMSPMDAIQSATSRAAVLLDMEG
                     KIGVVAPGAFADIIAMSGDPLRDIKVLESVQFVMKDGQVFRGK"
BASE COUNT         1732 a         2376 c         2871 g         1616 t
ORIGIN      
        1 aactcgtgct aaacgactgc agttcctggg tctgcgccgt gcgcgctttc ctataaactg
       61 gtaggcgatt cccgttcaaa aggactcttc catgctgcgc aagaatcgtc tctttactcc
      121 cgggccgacg ccgctgctgc ccgcggcgca gacggccatg gctgccttca ctgcgcatca
      181 ccgcaccgcc gattttcgtg cgctgtttca gcgcgtgctc ggcgacatga aggagttcat
      241 cggcacaaaa aatgacgtgc tggtgttggc ttgctcgggc acgggcgtga tggaggcggc
      301 ggtcacgaat ctgacgtcgc ctggcgacaa ggtgttggtg ctgacagccg gcaagttcgg
      361 cgagcgctgg accgggctgg ccaaggcgtt cggctgcaat gtcgacgtgt tcagtgttcc
      421 ctatggcgag acgttttcgc tggacgatgt gcgcgctcgc gtaaagcctg acgtgcgcgc
      481 tgtgttcgtg caggcgaccg aaagctcgac cggggcgcgg catgatgtgc aaggcatcgc
      541 caagatcgtg cgggggcagg gcgatcagac gctactggtt gtcgatgcca tcaccgggct
      601 gggcacgacg cacctcgatg tcgatggctg gggcgtggac gtgacaatcg gcggatcaca
      661 gaaggccctg atgatgcctc cggggctggc ttactgcgcc gtgagcgagc gggcgtggaa
      721 gcgcatggac tcgaccactt cgccgcgtta ctacttcgat ttgcgcaagg aacgaaagtc
      781 ggcggccaag ggcgagacgg cttacactcc ggcgacttcg ttattcgcgg ccatgggagc
      841 ggcgctcgaa ttcgtacgcg gtatgggcaa cggcgatctg gccaaaggac gcgaggaact
      901 ggtcaacaat gccgagcttt gcgccgagat gacgcgggcg ggagcaaagg ctctaggcct
      961 gaagctgtat gcctcagcgc cggcggcggc gctgaccgcg atctcctcgc ccgacgggct
     1021 cgactccggg aagatcgtga aagagttccg cgagtcgttc gatgccgtgg tggccaacgg
     1081 ccagggagag atgaagggaa aaatcttccg catcgcgcac atcggctact acgactacct
     1141 cgatacagtt ggcattctgg gcgcgctcga acacgtgctg gctaaaactg cgggccggag
     1201 cgtcgaatat ggtgcagcgg tcaaggctgc gcaggaagtc tacgcgcgca gccttgcggg
     1261 tacacggcag ctggtcgggg cgtgaggact gcgaagcggg cagtcctcaa ttaggtgcgc
     1321 gtgcggctca gttgatcgtg aaggcgatcg gcgtcgaagt ctgggccgtc ataccgccgc
     1381 cgccgtatgg gcccgtaccc gtgacggcgg gattggtcac cgtgacgttc accataccgg
     1441 cattcatgat tgcggaagcc gggatcattg ctgtgacttt gcctgagttc gtccaagttg
     1501 tagccatggc cgcctggtta aagttgacga cggcggtgct gttgaaaccc gtgccgtcaa
     1561 cctccagcga gaaggcagcc gtcccgtggt tcacgtcggt tgggttcagt gttttaatca
     1621 caggcatggt gccggcggta ggcggggtca tggagtgcga ataaccgcac gccatcatca
     1681 gcgcgattcc ggccgcgagc aggatggtct tcagggtgtt tactgctttc atgattgatc
     1741 tccgaagggg tgatacccaa tggggagagt ttagaaatcc gggggcgtag aagtgtcagc
     1801 gcagtgtcaa aatcctgtca aatcgttgtt gcgctactgg gctcttatcc cgctgtaaaa
     1861 taatgcttta gctggtacat ccttatgcaa gaggaaactt atttgtgaag atcgtcgtgg
     1921 cagagaagat atcgccgtcg gcagtggcag aactgcagga accgggttgg acggtgctga
     1981 cggccgatca gctcgatggg aagctggcgg agcacctgga gtcggccgac gcgctgattg
     2041 tgcgctcggc ggtgcaggcg gatgcggcgc ttctgcagca tgctaagaaa ttgcgggtaa
     2101 tcgggcgcgc gggcgtggga gtggacaaca tcgacctcga ggctgcgacg cacaaaggca
     2161 tcgcggtgat gaatacgccg ggcgcaaatg ccgtggccgt cgccgaacag acgctgggga
     2221 tgatgctggc gatggcccgg cacttgtgcc gcgccgacgc gctcatgcac tccggcaaat
     2281 gggagaagaa atcgctgcaa gggaccgagc tgcgcgggaa gactttgggc atcgtcggcc
     2341 tgggaagaat cggcatggaa gtggcccggc gcgcgaaggc gtttggcatg aagctggtgg
     2401 cacacgatcc gtttgtgtcg gtcgctgtgg ccaaggatca ggaaatccgg ctggccgggc
     2461 ttgatgagct ctatgccgcg gcggattaca tcacgctgca cgtgggtctg acgccacaga
     2521 cggcgggcat gatcaaccgg gactccatcg cgaagatgaa gaaaggtgtg cggctggtga
     2581 attgcgctcg cggcgagctg gtgaacgaga cagacctggc ggccgcgctg aagagcgggc
     2641 acatcgcggc agcggcgctt gacgtttttg cggacgagcc gctgaagaac tcgccgctgc
     2701 aggcgctcga aaatgttgtc ctcaccccac acgtcggtgg ttcgaccttc gaagcgcagg
     2761 aagctgtggg cgtgcagatc gcgcagcagg tcaaggaata tctgaagcat ggcgtgatcc
     2821 agaacgcggt gaacgtgccc tcggtctcgg ccgaagagta cgccgccatg cagccctaca
     2881 tcgtgctggc cgagcgtatg ggtgcgtttc tggcgcaggt ttccgaagga acaatcgaag
     2941 aaatctcgat tcgctatagc ggacatattg ccgagggcaa gacggaactg attcgcaacg
     3001 gggccatcaa gggcattttg aaccaggcgc tcgaggagaa ggcgaacctg gtgaatgcgg
     3061 cgaccatcgc cgatgcgcgc ggcctgcgcg ttctcgaagt gcgcaaagag aaggcgtcga
     3121 tcggcggagc gggcagcgtg atctcgatct tcctgaagag ttcgaccgaa gagcacctgg
     3181 tcaagggcgc cgtgctgcat gagacggcgc cccggcttct gcatgtggat ggcatcgacg
     3241 tggaggcgcc tctcgagcgc aatctgatct acctgcgcaa ccgcgacgtg ccgggtgtga
     3301 tcggcaaagt gggcacgatt ctgggcgcag aatcaatcaa catcgccgat ttttcactgg
     3361 ggaggcgcag tgcggagaaa gactcggccc aaccgcgcga ggcgattgcc gtggtccacg
     3421 tggacggcca tgtgccggac cgcgtgctat tcaagctgcg gcagattccg gcggtgcaga
     3481 aagcgaaggc ggtgaggttg ttctgaaatc cccacttctc gcgcggagaa cgcgagagaa
     3541 atggggcacc cgcactcatc aactcggcgt gctcggcgac ctcggcggtg agtggatcta
     3601 ctgctcggtt tcgtcccgcc gcagtttcct gctctcggcc agcaacgaca tggagtgcat
     3661 caggttctgc agggtgacga tgccgaccag gcgctggtct tccaccacag gaatgatgct
     3721 ggcgctgcgc gacgtgagtt tgcggaaggc ggagccgagc gaatcctgcc gcacggacac
     3781 atcgaaaatc ttgttcatga ccgcctgcac gtagccgttg ccttcggcgc gcagagcgtc
     3841 gagaatgcgc tgccgcgaga tcacgcccac catgtcgctg ccgcgcacca cgggaaaatc
     3901 gtcctgcagg gaatgaaccg ccttttccag tgcgtcctcc agcgtgtcgg cgggtgagag
     3961 cgtggcgaag tcggtgagca tgacttcttc caggcgaacg ttgtcgagta ccgactggaa
     4021 cacgagcgcg cgctcttcca gttgtgcggc agagaacacg atgaggccaa ccatggtcag
     4081 ccagttgtcg ctgaacaggc ctgcaaccat cagcaccatg gcgatcgcat tgctgatgga
     4141 aacggcgcga cggctggcgg cggaagcgtc gagggtgcgg gagaagaacg cgcgcaggat
     4201 gcggcctccg tcgagtggat aagccggcat gaagttcaga agggcgagat aaaggttggc
     4261 ccagaccacg ctgcgtggca agttccgcgc ggtaagaaag ggccatttga agagctcgat
     4321 attgcggccg gaggcagcta tgatcgcggc ggccagcgat gccagcgcca ggctcaccaa
     4381 tgggccaacc acggtgaggc gaatttcgcg tttccacgcg agcgttgacg gctgcttgac
     4441 ggagcgggat tcgtcataca gcgtaacgcc ggtcagcggc agcagaatca cggcttttgg
     4501 aatcaatccg aagcgccggg cggccagcat gtgtccggat tcgtgcgccg ctacacaggc
     4561 gagaataatg ccggtcagta tcagatcgcg cgatccgttg gcgtcgcctt gatgcgcagc
     4621 gtagtcggtc cagaagatga acaggggaag aagaaagaac gtcaggtgca gacgcacgtc
     4681 taccccgaac aggcgtccga tcggaatcga ccagcttcgc atgggtgtta gctactttta
     4741 ttctacatgg atggttatat tgggttagac gacagaaggt cgatgtccat gcgggtcatc
     4801 gcgggcaaat accggagtcg gccccttcgc tcgaagcggg gcatggacat tcgtcccacc
     4861 tcggaccgcc tgcgagaaac gctgttcaac gtgctcacgg cgggaaatcc agcggccctg
     4921 gaaggaagcg tttggctcga tctgtacgcc gggacgggag cggtcgggat cgaggccctg
     4981 agccgcgggg cacgccaggt ctactttgtc gagtcgtcgg ccgaggcggc ggaggtgatt
     5041 cgcgccaacc tgaagagcct caacatcagc agcggattca agctgctgca cgatgaactg
     5101 ccgcgtgcgt tctggcgcat ggagcgggag cacgtggcgg ccgacgtggt gtttgtcgac
     5161 cctccgtacc gcatgaagga ggcttaccgg gacacgctgc gcgcattggc cgagtcgccg
     5221 gtgatctggg caatgtcgat ggtgatcgcc gagcacgaga aaaagtttga tccgggagag
     5281 gaattcgggc cgctgcgcag atttcgcaag ctcgtgcagg gcagcgtggc gttgagtttc
     5341 tatcgcaggg ccagtgcggc agatgtgcgg acctgacgct cggaatcgat cttcgggttg
     5401 atgttgggga acggaccggc gtgccagcac cgctacatca tcaggtcatc aggcgcacga
     5461 gcgatgggat gctgcatcac tgcgagttcg tttttgacca tgttcagccc gaaaacgcag
     5521 ccttcaaagt tgcgtgacag agtggcgaac agcggcgcta ccttggcata ttcgaagtgc
     5581 tcgaacttgg agacgatgta gtagctttct ttcccggcct tgatccactc gacgaggttc
     5641 tccagacgat gacggcgcag gcggaagtgg ttgatgcttt cgggaaacat cccggcgaaa
     5701 aacagggtgt agtcgccgat gtgcttgcgc acctgtcgct cgcgatcgaa tgatggggcc
     5761 gggccgtaga caggatcgga ctcgagcagc atttcgccga cgtcggcgag tggacgattg
     5821 gccgcatcac gaatcttata gagctgatca gcttcgcaga actccgccag caggtgcgag
     5881 acgtaagcca cgatctgagg atcgcgaatg ccgatctccc cggcgtagtg ccgtcccacc
     5941 agatcctgaa acagctgctg cagcggatgc gactccggga tcatgccgtt gggctccctg
     6001 aacgccgtgc ctggcacgga gtttcagcta cttgcgtgag taaatatccc agattcattt
     6061 ccagcgcaag tgacttttgt ttatcgtcac aattcgtttt gctgatcagt tagaacccgc
     6121 gacttgcggg cgagttgccg catctatttg ttggcactgt ggcgaggagg ctgctaaaac
     6181 agcgctaaca gcgggctgtt gagggatctc aggcaagccc gccaaccatt cgggtccacg
     6241 aaatcaccaa ccacggagga gcagatggaa aggcagaaca tatcgagcgg ggcgccctgg
     6301 gagccggtgg tcggctactc ccgggcagtg aaggttggac aatacgtcca cgtgtcgggt
     6361 tcgacggcga ttggagccga cgggaaaatc gtcggcgtgg gcgatgtcta tgcccaggcg
     6421 gtgcagacgt tgaagaacat tgaaagcgcg ttgaaaaagg cgggagcgag catgaaggat
     6481 gtagtgcgca cgcgcatgta cgtgctcaac attgccgaat gggagaaggt cgggaaggcg
     6541 cacggcgaat tctttcgtga tgttcgcccg gcgacgacga tggttcaggt gagcgcgctc
     6601 atctcgcccg atatgctggt cgagattgag gccgacgcag tgttgcccgg aacgcgtgct
     6661 taatcagctc agcggttttg acatctccac gaagtgacaa ggcaccttcg ggttcaagcc
     6721 cggagtgaac ggggctgtac cggtctcaac atatccacga tgccgataga aggccgggag
     6781 ctcgcggcgc acgttgacga tgcgcagatc catgaagcgg cagccggccc gggcgcagta
     6841 attctcggcc gcgttcatca gttgcgagcc cagcccagcc ttctggcggt ccggatcgac
     6901 cgacagcaga ccaagatagg ctctatcgcc gcgcaactcg atgtaaacac atcctgcaag
     6961 caccccgccg tcctcggcga caaggaactt tcccctttcg agcagtgagc ggaccatctc
     7021 cagatcgacg cgatcgcggt cgataagaaa cgactcggcg cggcggaaag cagcgttgat
     7081 cacagcgatg atggccggtg catccttcac ttctgcgacc cgctcctgca cgatgccgtc
     7141 ctttggttgg caaagttacc cgccagctct tctcccaagg gtatagaatc cttgccgcca
     7201 tttcacctgc tgaccttcga cgtaggactg gaaattggcg agttctttcg ggggaggccc
     7261 aaattgaaga tctctcgctg tctgtttgtt cttgcccttg ctgttcctgt cattgctcag
     7321 caaacgcgcc cggcggcggg gcaacaagtg actgtcattc gggcgggaac gctgatcgac
     7381 ggcaaatccc agcagccgaa gcgcgatcag gtcatcgtgg tgcggggaaa cagcattgaa
     7441 agcgtcggcg atgccgcgag cgcgaagatc ccgaccggcg cgacggtcat cgatctttcc
     7501 cgtcaaaccg tgctgcccgg gctgatcgat tcccacaccc acatttttct gtggggtgag
     7561 gatccggaga agggcggata cgacgtcaac atcctgatgg cgccgttcgg gctgcgcgca
     7621 gctcgcgcca ccgtggccgt gcgacgggcg ctggagcagg gattcaccac gttgcgcgac
     7681 gtcgagaccg aaggggcggg atacggcgat atcggaatca agcaagcgat cgaagatgga
     7741 atcatcccgg ggccacggct gttcgtggcc acgcgcgcca tttcgacgac cggcggctac
     7801 atgctggaag gctatgctcc tgagctggtg atgccgaagg gcgcgcaaat cgccgatggg
     7861 ccggtcgaag cccgcaaagc ggcgcgcgag cagcttgatc acggcgccga ctggatcaag
     7921 gtttatatga cgcaccgctc ctgggtgggc aagaatggcg aactcgtgtc gcagccgacg
     7981 ttcaccgtcg acgagctgaa ggcaatcgtc gacgagaccc atggctgggg caagaaagtg
     8041 gcgtgtcatg cctatggcgg gattggcctg caccgcgcgc tcgacggcgg atgcgactcc
     8101 atcgagcacg ggcttgatct cgacgatgcc gccatcgcgc agatgctgaa gcaaggcacc
     8161 tggtacgtgc caaccctcag cccgtactac tacgattggg ctccggccga tactcccaac
     8221 ggaaagcggg accggctgcg cgctgcggtg catgagcctt ctttcaaaaa ggcgctgaag
     8281 gcgggggtaa agatcgtgtt cggcaccgat atgggcggaa ttccctggac cgaccccatc
     8341 gctcaggaat tttcgcgcat ggtgagcttc ggcatgtcgc cgatggatgc gattcagagc
     8401 gcgacgtcgc gcgcggccgt cttgctcgac atggagggca agatcggcgt ggtggcgcca
     8461 ggagcgttcg ctgacattat cgcgatgagc ggcgatccgc tgcgcgacat caaggtgctc
     8521 gagagcgtgc agtttgtgat gaaggacggg caagtgttcc gcgggaagta aggcgattcg
     8581 taccctgcgc ggcct
//
LOCUS       DASPUL010000029        10687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_689702, whole genome shotgun sequence.
ACCESSION   DASPUL010000029 DASPUL010000000
VERSION     DASPUL010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689702"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            587..2596
                     /locus_tag="VEJ00_00650"
     CDS             587..2596
                     /locus_tag="VEJ00_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW99693.1"
                     /translation="MVVAAASMATGTQTALSSAHLRVLLVGRQEEDFFLIREILQRTG
                     NVVAAELDHAASLEEATAMLRNGAYDLVLFEHETADAEAVRLLVEFLQGGVSVPFLLL
                     TEDADERRIAEIIGAGEWNYLSRSQLDGATLIRTIRSTVALHSMRQRKQHAEESLRKL
                     SRAVEQSADAVMVTDRRGIIEYVNPGFESLTGYTSAEVCGLTPRFLKSCEQGPEVYQE
                     LWKTILAGNVYRGIFVNRKKTGELFRVEESISPVRDGAGNITHFISNTRDLTERLRLE
                     AQLQQAQKMDAIGHLAGGVAHDFNNLLTIITSYAELSLDAILGNSDLQGKIEEILRAA
                     RRAAELTRQLLAFSRKQPQSLRVVDLNEVIAGIARTLPRLIGEDIELSFVPGCGMGRV
                     RVDPVQIEQILLNLAVNARDAMPEGGHFRIETSAVELDEDYVDRKSAAIPLGRYALIT
                     ASDSGAGIAREHLPHIFEPFYTTKSSGQGTGLGLATVYGIVKQHQGYVWAYSESGMGT
                     VFKIYLPCWTAPDQAERPEAEKAEAIVRGAETVLLVEDELAVRRATAEFLGRQGYTVL
                     EAKDGADALALVKNFGHTIHLLVTDVVMPNMSGGQLAKELLQLRPETRIVFVSGYAGK
                     TVLDHKVFDVATNFLQKPYTLKQLSSKVREALDNSREAARTPADD"
     gene            2808..4613
                     /locus_tag="VEJ00_00655"
     CDS             2808..4613
                     /locus_tag="VEJ00_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXW99694.1"
                     /translation="MPFSNLADSIPKPNRASLVILVVLWLAIYAAGMFTPPLLDDVDT
                     IHAEAAREMLLRHDWVTLYTNGLRYLEKAPLMYWGLAASYEVFGISDWSTRLPLMLGV
                     LALILATYGLGNYTLGERGGFYSGLALVTSVGPYVFTRFLIPDVLVGLWLVISYFFFL
                     RSLEEETPSRLTCWGFAAACALNVLTKGLIGLVFPIATIGLYLVLTRNLRHLAKLRLL
                     SSTIIFLVLAAPWHILAALRNPPQGQARGFLWFYFVNEHINRFLGTRMPPGYDTVPLV
                     LFWAFLVLWLVPWAIFLPQALKGVPWSKLSAPDRRGRANLLFLLWALVILLFFSFSTR
                     QEYYTIPALPGVALLVGGWLAQESAPEASDADRRAGRISSLVFMIVGIVGATIGLALF
                     SRVHQPPAGVDLADLLKRNPQDYDFSLGHVLDFTADALGVFRGPLLGASLSFLFGTSL
                     NWWLRRRGRPAWGNAALSLMMVGLLTCVHVSFARFSPILSSHDLAVAIQKEYRPGDLI
                     VIDGEYHQASTLNFYTHVPVRVLHAPSGNLWYGERFPDAPHVFETQQSLAALWAGPKR
                     VFLWTDQDDPKELAGMPHFLLARSGGKAIFTNRPR"
     gene            4835..6061
                     /locus_tag="VEJ00_00660"
     CDS             4835..6061
                     /locus_tag="VEJ00_00660"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXW99695.1"
                     /translation="MNPGVLNAHGAELVWRALLLLAIAGSFTSSIYLIMTLVATARHL
                     RLARGAEAAADSTPPSSLPPVTIFKPIHGMEEQLAADLESFLQQDYPDYEVIFGVRDA
                     DNPAARIAEEIRARHSRIPSQVIVSGPPAWPNAKVFSLDKMIAASSRSFFIISDSDVR
                     VTPDFLRNVIPPLMDSKVGLVTCMYRGVPASDLWSRLEALGLSIEMSSGVMVADMVEG
                     MRFALGPAMAVRRDAIDAIGGIRAVADYYSDDFELGNRIWTKKYRVVLSHHIVENVLT
                     PRSPLRTLGDQLRWMKSTRYSRPLGHAGTGLTYAMPFGILGLIAGGVSGHWTWAIGLF
                     LLACANRIIQSVAVGWGVTRDPRALAFCWLYPLRDLFGFVAWMLSYTSRKFYWRGETY
                     CFGKGGRITPLERSST"
     gene            complement(6105..7277)
                     /gene="hpnI"
                     /locus_tag="VEJ00_00665"
     CDS             complement(6105..7277)
                     /gene="hpnI"
                     /locus_tag="VEJ00_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HXW99696.1"
                     /translation="MIQVTIRLLEIITLVGCLTSSIYYLVCLGSAASFLQERRAGKSA
                     PASRNLPPVSILKPLKGVDPKIYESFRSHCLQDYPEYEILFGVNDAADPAVACVRKLQ
                     QEFPERAIRLVVAPNILGANVKVSNLAQMLPEARYQHLIVNDSDIQVDRDYLRRVITP
                     LGDKNVGMVTCLYRGVAAPTLGSRLESLGISSDFCPGVLVARGLEGGLRFGLGSTLAF
                     RRSDLERAGGFASIVDYLADDYELGRRIADLGLRVVLSDVVVETYLPAYGVREFLAHQ
                     LRWARGVRDARAGGYIGLASTFGLMWGLLHLIAGHGAPWAWIVLASIALLRITMALAV
                     GKAVLHDGQALRYLWLLPLRDAIAVGVWIASFAGHTVTWRGDRFRLQRGKLVRIAS"
     gene            complement(7347..8609)
                     /locus_tag="VEJ00_00670"
     CDS             complement(7347..8609)
                     /locus_tag="VEJ00_00670"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW99697.1"
                     /translation="MMRKSEFGEIVQMASDTIRSNKLRSGLTVLGIVIGVAVVIGVSS
                     IGRGLDENVRQIVSSVGSDTVFAFHMEPINFGRPSEEMRKRRELTIDDAIAMQSLPHI
                     KAVSAALQYVHPELGVGAFAVKYRDRKAKNVILEGDQASASKIFDLPISSGRWIDESD
                     EEHHSPVVILCADTREELFGSEDPIGKEVNIEGQLFTVVGIAEKEKSVFGGGKNPDDN
                     KVIFPLSTLHKLHPELKQYWIMARATSHDDVPKAIDEIRELLRRRRKVPFDKPDNFAV
                     FTSDSISDVWNQLTGALFIGMFAISSVGLIVGGVGVMNIMLVSVTERTREIGVRKAIG
                     ARKRDILLQFTLEAIILTAAGGAIGILTGAILVWVIPVIWPSLPARLSMFWVSFGFSS
                     AAGVGLVFGIYPAWKAANLDPIESLRYE"
     gene            complement(8755..9987)
                     /locus_tag="VEJ00_00675"
     CDS             complement(8755..9987)
                     /locus_tag="VEJ00_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW99698.1"
                     /translation="MHLIESIRIALQSLWANKLRSVLTLLGVVIGVAAVIAVVTFVSG
                     INDYVAKKVFNLGADVFLIFKVSPVAIDVDHYLEGEKRKDLTIDDYRAVAEACRHCEL
                     VGASTRNESGHVKYGGQSISDTLVRGLTPGMATILDTDLSAGRMLNDTDLNNRSPVVV
                     VGNDIVEHLMPGMDPLGKEVLVDGWTYQVIGVAKKKGKTLGQSADNFVMMPITSCLKQ
                     YGSHDTSLRIAGKALSAGVSLSEAVDEARVALRAKRHDPLGGDDSFDIETNASLLSIW
                     SGLSNTFFMATIGIAAVSLVVGGIVIMNIMLVAVTERTREIGIRKALGARREDVLLQF
                     LIESVTLALFGGILGVLCGVGVAESVTALIGMPSSIKLWAVAAGLTVAAGVGVFFGVY
                     PARKAARLDPIVALRFEM"
BASE COUNT         2134 a         3178 c         3211 g         2164 t
ORIGIN      
        1 ccactttcca taacttcccc cagtgttgac gtaccgttga gaacaacatc ctcgccggca
       61 gcgagccgac gcgcgactaa accgcccgga tgcaggggta cggcagttta cccacagacc
      121 gaatcacacg ccttcagcaa ggagcaggga ccccgctggg gaatctatac gtatatctac
      181 tggacgcagt ttccctgaca gcggttgtct ggggaagtgc ggccttattg ctcacttaga
      241 cgcaaaaaat caggaatggt taacaaatca gggaccaaca ccagcaagag ggtgactaag
      301 cgtgggcctg gaggcgtaac cgcaatcccg acgcgggcaa ctgcggaaaa gtccaaatcc
      361 atcaattttc aagtcttacg gggcggagaa acgcccacac cgtagacgta cccccgggga
      421 acgacatcaa cctctttgtt ttgttgacct ttgcgggaat catgccaatg tcagcaactc
      481 gcctgattag tagggtccga tccgaaacgg tcttttgtgc ccatgtcgga atcccagcaa
      541 accaaaggga ggaacggtcg tcccgacgcc gggcgaaaac ctgccgatgg tagtggcagc
      601 ggcctcaatg gcaaccggca cacaaaccgc gctgtcctcg gctcacttac gggttctgct
      661 ggtcggccgt caggaagaag actttttcct gatccgcgag attctgcagc ggaccggcaa
      721 cgtcgtcgcg gccgaactgg accacgccgc ctcgctcgaa gaagccaccg ccatgctgcg
      781 caatggagcc tatgatctgg ttctgttcga gcatgaaacc gcggatgccg aggcggtgcg
      841 tcttcttgtg gaatttctgc agggcggagt gtcagtaccg tttctgctgt taaccgagga
      901 tgcggatgag aggcgcattg cggagatcat cggcgcggga gagtggaact acctgagccg
      961 ctcgcagctg gacggagcca cgctgatccg gaccatccgc agcacggtgg cgctgcacag
     1021 catgcgccag cgcaagcaac acgccgagga atccttgcgc aagctgtcgc gcgcggtgga
     1081 gcagtcggcc gatgcggtca tggtaaccga ccgcagagga atcatcgagt acgtaaatcc
     1141 cggttttgag tctctgaccg gatataccag cgccgaagtc tgcggcctga cgccacgctt
     1201 cctgaagtca tgcgagcaag gaccggaggt ctatcaggag ctgtggaaaa ccattctggc
     1261 gggcaacgtc tatcgcggca ttttcgtgaa ccggaagaag accggcgaac ttttccgcgt
     1321 ggaagagagc atcagtccgg tgcgggatgg cgcagggaac atcacccact tcatctccaa
     1381 cacccgggat ctaaccgagc ggctgcgcct ggaagcccag ctgcagcagg cgcagaaaat
     1441 ggatgccatc gggcacctcg ccggcggcgt cgcccacgac ttcaacaatc ttctgaccat
     1501 cattaccagc tatgccgaac tttccctgga cgcgattctg gggaattccg atctgcaggg
     1561 caagattgag gagatcctgc gggcggcgcg ccgggcggcg gagttgaccc ggcagttgct
     1621 cgctttcagc cgcaaacaac cgcagtcttt gcgggttgtg gacctcaatg aagttatcgc
     1681 cggcatcgcc cggaccctcc cccggttgat cggtgaagat attgagctaa gcttcgtgcc
     1741 tggatgcgga atggggcgag tgcgagtaga cccggtacag atcgaacaga ttctgctgaa
     1801 cctagcggtc aatgcgcgcg atgccatgcc cgagggaggc cacttccgca tcgagacctc
     1861 cgctgtcgaa ctcgacgagg attatgtcga tcgcaaatcg gctgcgatcc cattgggacg
     1921 ctatgctctc atcacggcga gcgattccgg agccggcatc gctcgcgagc atttgcctca
     1981 catctttgag cccttctaca cgaccaagtc gtccgggcag ggcactgggc tgggcctggc
     2041 gaccgtctat ggcatcgtga agcagcatca gggatatgtg tgggcttata gcgaatccgg
     2101 catgggcacg gtgttcaaga tctacctgcc gtgctggacg gctccggatc aagccgaacg
     2161 acccgaagcg gaaaaggcag aggccatcgt tcgtggcgcg gaaaccgtgc tgctggtgga
     2221 agatgagctg gcagtgcggc gtgcgactgc ggaatttctc ggcaggcagg gatacaccgt
     2281 cctggaagcg aaagacggtg ccgacgcgct cgctctggtc aagaactttg ggcataccat
     2341 ccatttgctg gtgaccgacg tggttatgcc gaacatgagc ggcgggcaac tggcgaagga
     2401 actgctccag ttgcggccgg aaaccagaat tgtgtttgta tcgggatatg ccggcaagac
     2461 cgtgctcgat cacaaggtgt tcgacgtcgc taccaacttc ctgcagaaac cctataccct
     2521 gaagcaactg tcatccaaag ttcgggaagc tctcgacaat agccgagaag cggcacgcac
     2581 cccggctgac gattgacgcg tctttcagaa ctttctccgc gatctccgcg gtatttctcg
     2641 gcgcgctctg ccatgaccag gattagatcg cagcgggcgc tgcgggctcg cagcgagcga
     2701 agggaaaacc tggacgcaag tttgatccgc tttttgtcag gccctataat cagcccaact
     2761 gcgcatgaat tctcgtgggc cccgcgacgg gggagcccgg aaccgcagtg cccttctcga
     2821 acttggccga ttccattcca aaaccgaatc gagcgtccct ggtgatcctg gttgtacttt
     2881 ggcttgcgat ctacgcggcc ggaatgttca ccccgccttt gctcgacgac gtggacacca
     2941 tccacgccga agcggctcgc gagatgctgc tacgtcatga ttgggtgacg ctctacacca
     3001 acggcctccg ctatctcgag aaagcgccgc tcatgtactg ggggctggcg gccagctatg
     3061 aagttttcgg catcagtgac tggagcacgc gcctgccgct catgctcggg gttctcgctc
     3121 tgattctcgc gacgtatggc ctgggaaact acacgttggg cgaacggggt ggattctatt
     3181 cgggcctcgc gctggtgact tcggtcggac cctatgtttt tacgcgattt ctgattcctg
     3241 acgtgctggt gggtttgtgg ctggtgattt cgtatttctt tttcctgcgc tcgctggagg
     3301 aggaaacacc atcgcggttg acctgctggg ggtttgccgc ggcctgcgcc ctgaacgtgc
     3361 tgaccaaggg actgatcggg ctggtattcc cgatcgcgac catcggtctg tatctggtgc
     3421 tcacgcggaa tttgcggcac ctggctaagc tgcggctgct ttcgagcacg attatttttc
     3481 tcgtactggc ggcgccgtgg cacatactgg cggccctgcg caatccgccg caggggcaag
     3541 cgcgtggatt cctctggttc tacttcgtca atgaacacat caaccgtttt ctggggacgc
     3601 gtatgcctcc gggctacgac accgtgccgc tcgtgttgtt ctgggcattc ctcgtgctgt
     3661 ggctggtgcc atgggcaatc tttctgcctc aggcgttgaa gggcgtgccg tggtcgaagc
     3721 tgtctgcgcc cgatcgtcgt ggcagagcaa acctgctctt cctgctgtgg gcgctggtga
     3781 tcctgctctt ctttagtttt tccacgcggc aggagtacta cacgattccc gcgctcccgg
     3841 gagtcgcgct gctggtggga ggctggctgg cgcaggagtc ggcgccggag gcgagcgatg
     3901 ccgaccgccg ggcgggacgg atctcttctc ttgtgttcat gatcgtcggc atcgtcgggg
     3961 cgaccattgg actggcattg ttctcgcgcg tgcatcagcc gccggccggg gtagatctgg
     4021 cggatctgct caagaggaat cctcaggact acgatttctc actgggccat gtgctcgact
     4081 ttacggccga tgccctggga gtgtttcgcg ggccgctgct gggggcgtcg ttatcctttc
     4141 tgttcgggac ttccctgaac tggtggctgc gccggcgcgg ccggccggcg tgggggaatg
     4201 ctgcactttc gttaatgatg gtcggtttgc tgacttgcgt gcacgtttcc ttcgcgcgct
     4261 tctcgccgat tctgtcgtcg cacgatctgg cggtggcgat tcagaaggaa tatcgccccg
     4321 gcgatctcat tgtcatcgac ggcgagtatc accaggcatc gacactgaat ttttatacgc
     4381 acgttccggt ccgcgtattg cacgcaccca gcggcaacct ctggtatggc gagagatttc
     4441 ccgacgcgcc gcatgtgttc gagacgcagc agtcgttggc ggcgctgtgg gcggggccca
     4501 agcgcgtttt tctgtggacc gatcaggacg accccaagga gttggcaggg atgccgcatt
     4561 tcctgctggc acgcagcgga gggaaagcga tcttcacgaa ccgcccgcgg tgaagaattc
     4621 cctggggcat cgaaaacaac ccggtttcct ttgcggcccc tgcggctttt cctcggcgat
     4681 ctttgcggta aaaagctttt gtcgcaaagt tcgcaaagga aaatccgcag agcacgcaaa
     4741 ggaaatctct aacaacgctc tgccctggct acgggctggg ccgcagagcg tgtctccccg
     4801 gttataatcg tgcttccttg acgactctct tctgatgaac cccggcgtcc tcaacgctca
     4861 tggagctgag ttggtttggc gggcgctcct gcttctggcg attgccggtt ccttcacttc
     4921 ctctatctac ttgattatga ccctggtggc gaccgcgcgc catttgcgcc tggcgcgcgg
     4981 agcagaggca gcagcggact cgaccccgcc gtcgtctctg ccgcccgtca cgatcttcaa
     5041 gccgatccac ggtatggaag aacagctagc cgctgatctg gagagctttc ttcagcaaga
     5101 ctatccggac tacgaagtca tcttcggcgt gcgtgatgcc gacaatcctg ccgcgagaat
     5161 cgcggaagag attcgcgccc gccattcgcg gattcccagc caggtgatcg tgtcgggtcc
     5221 gccggcctgg cccaacgcga aggtgttttc gctcgacaag atgatcgcgg cttcgtcgcg
     5281 ctcgttcttc atcatcagcg acagcgacgt ccgcgtgacg ccggattttc tgcgaaacgt
     5341 gattccgccg ttgatggatt ccaaagttgg actggtcacc tgcatgtacc ggggcgttcc
     5401 ggcgtcggat ctctggtcac gccttgaggc gctcgggctg tcgatcgaaa tgtcctcggg
     5461 agtgatggtc gccgacatgg tcgagggcat gcgcttcgcc ctcggtccgg ccatggcggt
     5521 tcggcgcgat gcgatcgatg cgatcggagg catccgagca gtggccgact actattctga
     5581 cgatttcgag ttgggaaatc gcatctggac gaagaagtac agagtggttt tgtcgcacca
     5641 tatcgtcgaa aacgtgctga ctccccgctc tccgctgcgc acgctgggcg atcaactgag
     5701 gtggatgaag agcacgcgct actcgcgccc gttgggacat gcgggcacag gtctcacgta
     5761 cgccatgcca tttggcattc tggggctgat tgcaggtggg gtatcggggc attggacatg
     5821 ggcaattggt ttgttcctgc tggcctgcgc caatcgaata attcagtcgg tggcagtggg
     5881 atggggcgta acgcgcgatc cgcgggcgct ggcattctgc tggctctatc ctctgcggga
     5941 cctgttcggc tttgtggcgt ggatgttgag ctacaccagc cggaaattct attggcgcgg
     6001 cgaaacgtat tgttttggca agggaggaag gattactcca ctcgaaaggt cgagcacttg
     6061 aagtccgtcg ccctgagcag cgctcagggt caccatggcc gtggtcagga tgcaatgcgg
     6121 accagcttgc ccctctgcaa gcgaaagcga tccccacgcc aggtgacggt gtgaccggca
     6181 aagctggcga tccacactcc gacggcgatc gcgtcgcgga ggggaagaag ccatagatac
     6241 cgcagcgcct gcccgtcgtg taacactgcc ttgcccaccg ccagcgccat cgtaatgcgc
     6301 aacagcgcta ttgatgccag cacaatccac gcccacggag ccccatgtcc cgcgatcaga
     6361 tggagcaaac cccacatcaa cccgaaggtg gatgccagac cgatatatcc tccggcgcgc
     6421 gcgtcacgca cgccgcgggc ccaccgcaac tgatgcgcca ggaactcgcg cacgccgtag
     6481 gcgggaaggt acgtttcgac cacgacgtcg gaaagcacga ccctcaagcc gagatcagcg
     6541 atgcgccggc ccagttcgta gtcgtcggcc agatagtcga cgatggaggc gaacccaccg
     6601 gcgcgctcca gatcggaacg gcggaaggcc agcgtggaac caagtccgaa gcgaaggccg
     6661 ccctccaggc cacgcgccac cagcacgccg gggcagaagt cgctgctgat ccccagcgat
     6721 tccaggcgtg aacccaaagt cggcgcggcc acaccgcggt agaggcaagt gaccatccca
     6781 acgttcttgt cgccaagcgg cgtgatcacc cggcgcagat agtcgcgatc gacctgaatg
     6841 tcgctgtcgt tcacgatcag atgttgatag cgagcctcgg gcagcatctg cgcgagattg
     6901 ctgactttca catttgcccc gaggatgttg ggggcgacta ccaggcgaat ggcacgctcg
     6961 ggaaactcct gttgcagttt gcgcacgcag gcaacggcgg ggtcagcggc gtcgtttacg
     7021 ccgaaaagga tctcgtactc agggtagtcc tgcaggcaat ggctgcgaaa gctttcgtag
     7081 atcttagggt ccacgccttt gagcggtttg agaatcgaga caggaggaag gttgcgcgag
     7141 gcaggggcgc tcttgcccgc acggcgttct tgcagaaacg aagcggcgct cccgaggcag
     7201 accagatagt aaatggaact ggtcaggcag ccgacaagcg tgatgatctc taaaagtctg
     7261 atcgtgacct ggatcatgcg ggatcgcagc cgccggagtt cacgcggatc gagctgagag
     7321 ccgtcggcta tttaccgaag gctgcattac tcgtagcgca acgactcaat cggatcgaga
     7381 ttcgccgctt tccacgccgg gtaaatgccg aagaccagcc cgaccccggc cgcagaactg
     7441 aagccgaacg aaacccagaa catggagagg cgcgccggca gcgacggcca tattaccgga
     7501 atcacccaga ccagaatcgc tcccgtgaga atgccgattg cgccacccgc agcggtcaga
     7561 atgatggctt cgagggtgaa ctgcaggaga atgtcccgct tgcgcgcgcc aatcgctttg
     7621 cgtacgccga tctcgcgcgt gcgctcggtt acgctgacca gcatgatgtt catgactccg
     7681 acgcccccga cgatcaagcc tacgctcgag atcgcaaaca tgccgataaa cagcgctccg
     7741 gttagctggt tccagacgtc ggagatggag tccgaggtaa agaccgcgaa gttgtcgggc
     7801 ttgtcgaacg gtaccttgcg ccggcgccgc agaagttccc gaatctcgtc gatggctttg
     7861 ggcacatcat cgtgcgacgt ggcccgggcc ataatccagt attgcttaag ctcgggatga
     7921 agcttgtgca gcgtgctcag cggaaaaatc actttgttgt cgtcgggatt cttgccgcca
     7981 ccgaacacgg acttttcttt ctcagcaatt cccaccacgg taaaaagctg gccctcgatg
     8041 ttgacttcct tgccgatcgg atcttcgctg ccaaacaatt cctcccgggt gtcggcacac
     8101 aaaatcacca ccggagagtg atgctcttcg tcggactcgt cgatccatcg cccggaagag
     8161 attggcaggt cgaatatctt gctggccgag gcctgatcgc cttcgaggat tacattcttc
     8221 gccttgcgat cccggtattt caccgcgaac gcgccgacac ccagttcggg gtggacatac
     8281 tgcagcgcag cactcactgc cttgatatga ggcagggact gcatggcgat ggcgtcatcg
     8341 atggtgagct ccctgcgttt gcgcatttcc tcggagggcc ggccgaagtt gatcggctcc
     8401 atgtgaaagg cgaaaacggt gtcggaaccc acgctgctca cgatctggcg aacgttttcg
     8461 tcgaggccgc ggccgatgga agaaacgcca ataacgaccg ctaccccgat cacgataccc
     8521 agcacggtca ggcccgatcg cagcttgttg ctgcggatgg tgtcggaggc catctggaca
     8581 atttcgccga attcgctttt gcgcatcatg actgctctgc ccatcgcttg tcattccgag
     8641 gagcgcagcg acgaggaacc tgttctcctg ccggcgacgg caaacagcag gttcctcgcg
     8701 ccgagagctg gcgctcggaa cgacaacatt ctggatatga caaacgcacc ccccctacat
     8761 ttcaaaccgc aatgccacga tggggtcaag ccgcgccgcc ttgcgcgcag gatagacgcc
     8821 aaagaacacg ccgacgccgg ccgcgaccgt caaccccgcg gccacagccc acagtttgat
     8881 ggacgatggc atgccgatca acgcggtgac gctttctgcg acgccgactc cgcagagaac
     8941 tcccaggatt ccgccgaaca gagccagggt cacggattcg atcaggaatt gcagcagcac
     9001 gtcttcgcgg cgcgcgccca gggctttgcg gatgccgatc tcgcgggtgc gttcggtgac
     9061 ggccaccagc atgatgttca tgatgacgat gccgccgacc accagcgaaa ccgcagcgat
     9121 gccgatggtc gccatgaaga atgtgttgct caggccggac cagatgctga gcaggctggc
     9181 gttcgtctcg atgtcgaaac tgtcgtcgcc gcccagcgga tcgtggcgct tggctcgcag
     9241 cgcaacccgc gcttcatcca cagcctcgct cagcgaaacg ccggcgctca gggctttccc
     9301 ggcaatccgc aagctggtgt catgggagcc atattgcttc aggcaactcg tgatcggcat
     9361 catcacaaaa ttgtcagcgc tctgaccgag cgtcttgccc ttcttcttgg ccacgccgat
     9421 gacctggtag gtccagccgt cgaccagcac ttccttaccc agaggatcca ttccgggcat
     9481 cagatgctcg acgatatcgt tgccgaccac cacgaccggc gaccgattgt tcaggtcggt
     9541 gtcgttcaac atgcggccgg cgctgagatc ggtgtccaag atcgtggcca tgcccggagt
     9601 gaggccgcgc accagggtgt cgctgatgga ctgcccgccg tacttgacat gaccgctttc
     9661 attgcgcgta gaagctccca cgagttcgca gtgccggcag gcttcggcca cggcgcggta
     9721 atcgtctatc gtgagatctt tgcgcttttc gccttccaga tagtggtcga cgtcaatcgc
     9781 cacgggcgaa accttaaaga tgaggaaaac gtccgcgccg aggttgaaca ccttcttggc
     9841 cacataatcg ttgatgccag agacaaaggt caccacggcg atcaccgccg ccactccgat
     9901 caccacgccc agcagcgtca gaaccgagcg caacttgttt gcccacagcg actgcagcgc
     9961 gatgcgaatg gattcaatga gatgcatacc gcagagtctt aaatcattag gggctgatat
    10021 cggccccgaa gcttgatgcc tcgacgcaag attaagtcta acagggcagc ctgggttgcg
    10081 gcgggacaaa gctgtgccag gctgccggtt tctccaatgc tgccggaacc ttgttcggga
    10141 gcggaccatt gcggcgcaat cagcgtcgct ccgcaggaat tcgagcacag gcttctcggc
    10201 tggtttcaga atcgaggcgc gaattcagtt cactgatcac agacaactgc cccctgacta
    10261 ctaagcgtgt tccctgcttt ttgcatttcc tgtgcacgct ctgtggagat ttagcctcca
    10321 gctcgggcgg gaatgttctg cggcgagatc gtcatcgcca aaacctgcaa cgccgcacac
    10381 cttcgcacgc acaaactgga agtctctctg atcggtgaca atccgggaag attggcggcc
    10441 gggtttttcc tcaactgcac caaaaatatt gaattgaccc gcagccgatt cgttcgtaga
    10501 gtctgattcg ctccacacaa aaaaccagct gaaaagttgc ccccggcggc gtgcaccgga
    10561 gggcaattga acgaaagcac gcatgcggta aaacatcgta ctcgggtcgc cgcgctatga
    10621 aaatggggga gtggaagacg taccgcacac gctttctggt caaggccaag cgcctcaatt
    10681 ccagcct
//
LOCUS       DASPUL010000030         3408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1238473, whole genome shotgun sequence.
ACCESSION   DASPUL010000030 DASPUL010000000
VERSION     DASPUL010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238473"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..720
                     /locus_tag="VEJ00_00680"
     CDS             85..720
                     /locus_tag="VEJ00_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HXW99699.1"
                     /translation="MRALLSSKWTKFAAFLSALAPLAVLSWRFYRRDLTANPLEFITH
                     TTGDWTLRFLVLTLAITPLRQLLRLPELIRFRRMLGLFAFTYGCLHFATYLTLDKFFD
                     FAEIWKDVAKRRFITVGFAAFLLMIPLAITSTAGWIRRLGGRRWRALHRAVYASAICG
                     VVHYYWLVKSDVRRPLVYGALVALLLVWRAVAWSKERGKNRPLETAEDTAP"
     gene            complement(756..1511)
                     /locus_tag="VEJ00_00685"
     CDS             complement(756..1511)
                     /locus_tag="VEJ00_00685"
                     /inference="COORDINATES: protein motif:HMM:NF022748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HXW99700.1"
                     /translation="MKGGKVTVEMKPRLQLVRWLALILGAAVACAPAFAAQRRGPSFH
                     PAAQKRKEQRQQRQQQKSEAKKGGNPGNNNRPPASPPSTVSPQGRDSMSGGNRNPGPP
                     FSGGAQASLERLRNMTPAERERFLQNNQNFQSKSPEEQARIRQNFQRWDRMMPQQQQA
                     MRDNERVWRNMTPEQRQHIRNDVMPKWRALPPERRQAMMQRLNILQNMPESARQQRLN
                     DPNFMRGMNAEDQSMLRDLSHLRGGATEPPPEL"
     gene            complement(1513..1986)
                     /locus_tag="VEJ00_00690"
     CDS             complement(1513..1986)
                     /locus_tag="VEJ00_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXW99701.1"
                     /translation="MSCNRMEHWMLEYLDGSLSENHRREADAHLETCDICRLRVKEFR
                     AVSGILDELPPVEPSAAFDIRMQARIAAEPKRSSFLAWLSPGPRVAFAASLLLLLVMW
                     SANRPTDVDDYIGHPPTVEEQFRMIKDLPVLEDYDVLANFEPLGSLPPAAQADQN"
     gene            complement(2004..2609)
                     /locus_tag="VEJ00_00695"
     CDS             complement(2004..2609)
                     /locus_tag="VEJ00_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXW99702.1"
                     /translation="MARSDVQLMLDVKAGDETSFALLLQRYRTPLVSFLYRMVRNREQ
                     AEDLAQEVFLRVYRARKDYVPSAKFTTWLFRIATNLALNSVRDTRYQQMEISIDQPVT
                     ADPEDGDERTVDVADKRPDIEQHLVEEARRKMIRHAIEKLPEKQRAAVLLHKYQELDY
                     GEIAKILQCSESALKSLLFRAYEALRVELAPLVALPGKQHG"
     gene            complement(2691..>3408)
                     /locus_tag="VEJ00_00700"
     CDS             complement(2691..>3408)
                     /locus_tag="VEJ00_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HXW99703.1"
                     /translation="VLATSLGLVAAGSNLLGGYFVFRRDWPHQFLRYFLALGAGYMLG
                     VALLDIIPESLRLAGPNALLYVVCGYFLVHFFEHTLAPHFHFGEETHGEAVRRAHAGT
                     TALIGLAIHTFFDGVAIASGFLVSTWLGAAIFIAIFLHKLPEGFAVASLMLATGRSRR
                     GALLASSLLGVATLAGVFVTDALRAQVGYALPLSGGVTLYVAASDLIPEVNRELSWRM
                     AVLVFAGPVILLILKRLFAA"
BASE COUNT          656 a         1065 c         1013 g          674 t
ORIGIN      
        1 ttcaacggct acgccgacca ggtcgcgaac ctctacaccg gaatggaccc gaaacgaatc
       61 tactgatcgg gagtcgaaac ctcggtgcgc gctctcctct cgagtaagtg gaccaagttc
      121 gcagccttcc tatcggctct cgctcccttg gccgtcctca gctggcgctt ttaccggcgt
      181 gacctgactg ccaatccgct cgaattcatc acccacacaa ccggtgactg gacgctccgg
      241 ttcctcgttc tcaccctggc gataaccccg ctgcggcagc ttttgcgcct tccggaactg
      301 attcgctttc gcaggatgct gggcctgttt gcctttactt acggatgcct gcacttcgcg
      361 acctacctca cgctggacaa gtttttcgac tttgcggaga tatggaaaga cgttgccaag
      421 cgccggttca tcaccgtggg ctttgcagcg tttcttttga tgattccgct cgcgattacg
      481 tcgaccgccg ggtggattcg gcgtctcggc ggaaggcgtt ggcgcgcgtt gcaccgcgcc
      541 gtgtatgcga gcgcgatctg cggagtggtt cattactatt ggctggtcaa gtccgatgtg
      601 cgtcggccgc tcgtctacgg cgcgctggtc gctctactgc tcgtctggcg tgctgtcgcc
      661 tggtcgaagg aacgcggaaa aaacaggccc ctggaaacgg cggaagacac cgcgccctag
      721 cgctttccgc tccagctatt gaagaacaaa acggactaaa gctcgggcgg aggttcggtg
      781 gctcccccgc gcaggtgaga caaatcccga agcatggatt gatcttccgc gttcatgccg
      841 cgcatgaaat tcgggtcgtt caggcgctgc tggcgcgcgg attccggcat gttctgcaga
      901 atgttcagcc gctgcatcat tgcctggcgg cgttccggag gcaaggcgcg ccacttcggc
      961 atgacgtcgt tgcgaatatg ttggcgctgc tcgggtgtca tgttgcgcca gacgcgctcg
     1021 ttgtcccgca tggcctgctg ctgttgaggc atcattcggt cccagcgctg gaagttctgg
     1081 cggatgcggg cctgctcctc gggcgactta ctttggaagt tctggttgtt ttgcaggaac
     1141 cgttcccgct ccgcgggcgt catgttgcgc aaccgctcga gcgatgcctg ggctccgccg
     1201 ctaaaaggcg gtcccggatt ccggttgccg ccgctcatag aatcgcggcc ctgcggagaa
     1261 acagtgctcg gtggtgaggc tggcggccgg ttgttgttgc ccgggttccc gccctttttt
     1321 gcctcgctct tctgttgttg ccgctgttgg cgctgctcct tgcgcttctg tgccgctgga
     1381 tggaagctcg gacccctgcg ttgcgcggcg aacgccggag cacacgccac cgccgctccc
     1441 aagatgagag ccagccatcg cacgagttgg agccggggct tcatttcaac cgtgaccttg
     1501 ccgcctttca cttcaattct gatccgcctg ggccgcagga ggcaggctcc cgaggggctc
     1561 gaaattcgca agcacatcgt agtcttccag caccgggaga tccttgatca tacggaactg
     1621 ctcctcgacc gttgggggat ggcctatgta atcatccaca tccgtcggcc ggttcgccga
     1681 ccacataacc agcaagagaa gcagcgaagc cgcgaaggct acgcgcggcc cgggcgaaag
     1741 ccaggccaga aaactgctgc gcttcggctc ggccgcgatg cgcgcctgca tccgaatgtc
     1801 aaaagcggct gaaggttcga ccggcggcaa ctcgtcgaga atgcccgaaa ccgcgcgaaa
     1861 ttccttcacc cgcaggcggc aaatgtcaca cgtctcgaga tgcgcgtccg cttcgcgccg
     1921 gtggttctcc gaaagactgc cgtcgagata ttccagcatc cagtgttcca tgcgattaca
     1981 gctcatgttc ctgttccctt ctctcatcca tgttgcttgc ccggcagcgc cacgagcgga
     2041 gccagctcca cccggagcgc ctcgtaagcg cgaaagagca aggacttcag cgcgctttcc
     2101 gagcactgca gaatcttggc aatctcgcca tagtcgagtt cctgatactt gtgcaacaga
     2161 accgcggcgc gctgcttctc aggcagtttc tcaatggcgt gtcgaatcat cttgcgccgc
     2221 gcttcctcga ccaggtgttg ctcgatgtcg ggccgcttgt cggccacgtc caccgtgcgc
     2281 tcgtcgccgt cttcgggatc cgcggtcacc ggctggtcga tggaaatctc catctgctgg
     2341 taccgcgtat cgcgaaccga attgagcgcc agattggtgg caatccggaa gagccaggtg
     2401 gtgaacttcg cgctgggcac ataatccttc cgcgcacgat agacgcggag gaaaacctcc
     2461 tgcgcgaggt cttccgcctg ctcgcggttc cgcaccatcc ggtacaggaa gctgaccagg
     2521 ggcgtccggt agcgctggag cagaagcgcg aacgaggtct cgtcgcccgc cttcacgtcc
     2581 agcatcagtt ggacgtcgga cctggccatg acctgcacat tctatcgaac ccattgaaaa
     2641 cgaaaaagtt gctggaattc cggatttcga ggggagcaag gtgcaaaggc ctaggcggca
     2701 aacaagcgct ttagaatcaa tagaatgacc ggtccggcga ataccagaac cgccatgcgc
     2761 cagctcaact cgcggttcac ttccggtatg aggtcggacg ctgcaacgta cagcgtcacc
     2821 cctccggaaa gaggcagcgc gtagcctact tgtgcccgaa gagcatcggt gacaaagaca
     2881 cccgcgagcg tcgcaacccc cagcagggag gaggcaagca gtgccccgcg gcgagaacgc
     2941 cccgtggcca gcatcaatga ggcgactgca aatccctccg ggagtttgtg cagaaagatc
     3001 gcaatgaaaa tggccgcgcc gagccacgtg gacaccagga acccggaagc aatcgccacc
     3061 ccgtcgaaaa acgtgtgaat cgccaggcct atcaacgctg tcgtccccgc atgtgctcgc
     3121 cgcacagcct cgccgtgagt ctcttcgccg aaatggaagt gcggggccag agtgtgctca
     3181 aaaaagtgca ccagaaaata gccgcaaacg acgtacagca gggcgtttgg cccggccagg
     3241 cggagagatt ccggaatgat gtccaggagt gcgaccccaa gcatgtagcc cgctcccagc
     3301 gccaggaagt agcgcagaaa ttgatgaggc cagtcgcgcc ggaaaacaaa gtacccgccc
     3361 agcagattgc tgccggcggc aaccaggccc aacgatgtgg ccaggaca
//
LOCUS       DASPUL010000031         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_4556, whole genome shotgun sequence.
ACCESSION   DASPUL010000031 DASPUL010000000
VERSION     DASPUL010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4556"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /gene="chrA"
                     /locus_tag="VEJ00_00705"
     CDS             complement(<1..1098)
                     /gene="chrA"
                     /locus_tag="VEJ00_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HXW99704.1"
                     /translation="MVRLFLRLGFTAFGGPAAHIALMEDETVTRRGWLTRQQFLDLLG
                     MANLLPGPTSTEMSIFLAFRRASWAGLLLGGACFILPAVLLTGLLAWAYLRFGALPKI
                     ASILYGIRPVVVAIVLQALWRLGRSALKSGFLLAIGAGALALGLLEVGPVWILLLSAV
                     LAASAEWGHRWGRSAVAVAAGPWIAGASGAVTLTGVFLVFLKMGFVVFGSGYTLLAFL
                     RADLVGRLHWLTERQLIDAVAVGQFTPGPVFTTATFIGYLLAGPKGAGVATAAVFAPA
                     FLFVAATGGLLPRLRKSATVGAVLNGVNAGSLGLIASVGFFLGRSAIIDLPTALIGVA
                     SAVLLLALRLNPTWLILAGAAAGALLGRGVPI"
     gene            1527..>2633
                     /locus_tag="VEJ00_00710"
     CDS             1527..>2633
                     /locus_tag="VEJ00_00710"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXW99705.1"
                     /translation="MSKIGTQVLRAVDAVVCKTAGITFNTVQFFNKYRPNASFTPKWS
                     DKPLLKSWEKTKPTLGWPRQTDSLCPDCVKEAREAIIKGQKDWRDLMSEKVGEIKAQI
                     IERDGQVWMVKDCPLHGHYEDLMAVDAKFLQWIESQFPGRDIPAHNDEDLHKHGSSTV
                     RYGRGSVLTVDLTNRCNMMCDPCFMDANQVGYVHELAWDEIQEILDNALRIKPRRQMS
                     VQFSGGEPTMSPYFLDAVAYARKIGYNSVQAATNGIEFAKSKEFSRKAFEAGMRYAYL
                     QFDGIGNDANSHRQVGNLFDVKLRAIENMHEAGIEIILVVTIVNNVNNDQVGSIVKFA
                     MENPRKISFVAFQPVSFTGRDEDITPERRLRQRYT"
BASE COUNT          584 a          832 c          824 g          393 t
ORIGIN      
        1 aatagggacg ccgcgcccga gcagcgctcc tgcggctgcc ccggcaagaa tcagccacgt
       61 cgggttgagc cgcagcgcga gcagcagcac cgcgctggcg accccaatca gcgcggtagg
      121 cagatcgatg atcgccgacc ggccgagaaa gaaccccacg ctcgcgatca gccccagcga
      181 gccggcattt actccgttga gaaccgcgcc gaccgtggcc gacttgcgca aacgcggaag
      241 caggccgccg gtggccgcga cgaacaggaa tgccggggcg aagacggctg cggtggcgac
      301 gcccgcgcct ttcggtccgg ccaggaggta gccgatgaac gtcgctgtcg tgaatacggg
      361 acccggagtg aattgcccca cagctactgc gtcaatcaac tgccgctccg tgagccaatg
      421 cagccgcccg acgaggtcgg ctcgcagaaa cgcgagaagc gtgtacccgc tgccaaaaac
      481 gacaaagccc atcttgagga agaccaggaa caccccggtc agcgtgacgg cccccgacgc
      541 cccggcgatc cagggccccg ccgcaaccgc aaccgcgctg cgcccccagc gatggcccca
      601 ctccgcactg gccgcaagca cggcgctgag cagcagaatc cacaccggcc cgacttccag
      661 gaggcccagg gccagcgcgc cggcgccaat ggccagcaag aacccactct tcaaagctga
      721 gcggcccaag cgccaaaggg cctgcagtac aatagcgacc acgacgggac gaatcccgta
      781 aagaatactc gcgatttttg gcagcgcccc gaagcggaga taggcccagg cgagcaggcc
      841 cgtcagaaga acggcgggca aaatgaagca ggccccgccc agaaggaggc cggcccagct
      901 cgcgcgccga aaagccagaa agatggacat ttcggtcgaa gtcgggccgg gcagcaggtt
      961 cgccatcccc agaagatcca agaactgctg cctcgtgagc caaccgcggc gggtcacagt
     1021 ttcgtcttcc atcagggcaa tgtgcgcggc cggccctccg aacgccgtga agccaaggcg
     1081 caagaaaagg cgaaccactt cgccgagatt cccgcgggtc cgtcccggcg cgggcgcgac
     1141 tggttcgcct ggagttgtag tggacaagcg gcctaggata agccgcgaag ccccggtctg
     1201 caaacggctg gcggaagtgc cgccgcttcc ccaaattcgg ccggccgaag caataaaatt
     1261 gaagatcggc ggcgggccgg cgcctgccgt tcattgccta gaatcgcttt tcactgccgg
     1321 ttttgcgcca ctcgccgcat agggctggat tctggtgttg taaggtttaa gctatacatg
     1381 ggaaaagact tataggtccg agcgccgggc cgtgctaagg tagcggaagg taagaaggtg
     1441 ctcgcggggg cattaaggcg agccgggtcc tggtttttgg ggcgtaatcc agcaaggcta
     1501 acttaacttt gggggcgatt ggtgtcatgt ctaaaatcgg cacacaggtt ctgagggctg
     1561 tggatgctgt ggtctgcaag accgcgggaa tcacattcaa cacggtccag ttcttcaaca
     1621 agtacaggcc gaacgcttcg ttcacgccga aatggtcgga taaaccgctc ttgaagtcgt
     1681 gggaaaagac caagccgacg ctcggctggc cgcgccagac ggactcgctt tgcccggact
     1741 gcgtcaagga agcccgcgaa gccatcatca agggccagaa ggactggcgc gacctgatga
     1801 gcgagaaggt gggcgagatc aaagctcaaa tcatcgagcg cgacggccag gtctggatgg
     1861 tcaaggactg cccccttcac ggccactacg aggacctgat ggccgtggac gcgaaattcc
     1921 tgcagtggat cgagtcgcag ttccccggcc gcgatatccc cgcgcacaac gacgaggacc
     1981 tccacaagca cggctcgagc accgtgcgct acgggcgcgg ttcggttctc accgtggacc
     2041 ttaccaaccg ctgcaacatg atgtgcgatc cctgcttcat ggacgccaac caggtcgggt
     2101 acgtgcacga actggcgtgg gacgaaatcc aggagatcct ggacaacgct ctgcgcatca
     2161 agccccgccg ccagatgtcc gtgcagttct cgggcggcga gcccacgatg agcccctatt
     2221 tcctggacgc ggtcgcctac gcgcgcaaga tcggctacaa cagcgtgcag gccgccacca
     2281 acggcatcga gttcgccaag agcaaggaat tctcccgcaa ggcgtttgaa gcgggcatgc
     2341 gctacgccta cctgcagttc gacggcatcg gtaacgacgc caacagccac cggcaggtgg
     2401 gcaacctgtt cgacgtgaag ctgcgggcca tcgagaacat gcatgaggca gggatcgaga
     2461 tcatcctggt agtgacaatc gtcaacaacg tcaacaacga ccaggtaggc tcgatcgtga
     2521 agttcgcgat ggagaatccc aggaaaatct ctttcgtggc cttccagccg gtttcattta
     2581 ccggccgcga cgaagacatc acgccggagc ggcgtttgcg ccagcggtac acg
//
LOCUS       DASPUL010000032         3483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_416162, whole genome shotgun sequence.
ACCESSION   DASPUL010000032 DASPUL010000000
VERSION     DASPUL010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416162"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VEJ00_00715"
     CDS             complement(<1..554)
                     /locus_tag="VEJ00_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXW99706.1"
                     /translation="MIRTIIMLTFWSLAAPFAALIGFPWTFLTGDIRLLYRMFTWGAY
                     VGVQLAGVKIEIIGRDQFDHSRSYIFMTNHVSNLDPPIQIPLIPRRTSVMVKKELFKT
                     PILGPAMRLASLVPVDRGNRDAGIEAVAAAKEVVQQGINMTIYVEGKRSFDGKLLPFK
                     KGPFYLAMECGVPIIPMTIAGTHYA"
     gene            complement(583..2412)
                     /locus_tag="VEJ00_00720"
     CDS             complement(583..2412)
                     /locus_tag="VEJ00_00720"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99707.1"
                     /translation="MTVQPPVRQWLRRALCAVALAASIAYAQAPVGYNISLASPSQHL
                     VEVQIALPAGATQRELQLPVWNALYQVRNFAQYVNWVHARDRSGKVLAVRELNQSRWQ
                     IQGAENGAVVEYQIFVNTPGPFAAELTPHHAFFNLAQILMYPVDARNSPLHVHLDHIP
                     AGWRAATPLMSVSGVDFDAAGYDRLVDSPVEISDFRESDFDESGGHYRVIVDADPSDY
                     DMEKIVAALHKIVAAATTWMSDRPFDTYMFLYHFPHGPAGGGMEHAFSTAIDINADVM
                     RQTLQGLTSITAHEFFHLWNVKRIRPETLEPVDYTRENFTRALWFCEGVTSTAESIIQ
                     LRAGLLDEGFYLYRLGAQITELERRPAHLTQSAEESSLDAWLEGSSYYRRPDRSISYY
                     NKGELLGVMLDLAVRDASHGQASLREVLQWMNANYAKQGRFFNDSEGVRQAAEAVTHA
                     DLGWFFAKYVAGKEEIPWNDFFRSVGLRVATETTTVADLGFSASRDFDAPMTVDAITP
                     GSEGERAGLKVGDSVLQIQGKPPGQELRPDFLRLKPGDALTLRIRGENGERELKWKVG
                     GRQEVSYEVKDLDHVTPEQRARRTAWLNGEAQAAAQTSSGPIR"
     gene            complement(2409..>3483)
                     /locus_tag="VEJ00_00725"
     CDS             complement(2409..>3483)
                     /locus_tag="VEJ00_00725"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HXW99708.1"
                     /translation="SMLSWIFHSAGLEPSFLIGGIAENFGSSFHLGKGKYFILEGDEY
                     DTAFFDKGPKFMHYFPDAVIVTSVEFDHADIYKDLDAVETAYRRLVNLVPRRGRIIAF
                     DGAVGEATESPSLERCLAKAFCPVERYGAGSRAHWKVTNLSLDPGRTSWSVMHDGQPW
                     SEFEFALAGEYNVWNATAAAALATSCGVPKDAIAEALRTFKSVKRRLEVKAEVNGITI
                     IDDFAHHPTAIAGTLKALRARYAGSRLWAILEPRSNTLRRRVLQNDLARSLALADEIV
                     VANVFRSEAVPENERLELPTLAAEIERNGRRARLLADADAIVQTVAPEMRAGDVVAIL
                     SNGGFGGIYEKLPAQLKKLVHTR"
BASE COUNT          675 a          974 c         1155 g          679 t
ORIGIN      
        1 gcgtagtgcg tgccggcgat ggtcatggga atgatcggaa cgccgcactc catcgcgaga
       61 tagaacgggc ctttcttgaa gggcaggagc ttgccgtcga aggagcgctt gccttccacg
      121 tagatggtca tgttgatgcc ctgctgcacg acttccttgg cggcggctac ggcttcgatg
      181 ccggcgtcgc gattgccgcg atccaccggg acgagtgaag ccaggcgcat ggcaggaccg
      241 agaatgggag ttttgaagag ctcctttttc accatcaccg acgtgcggcg gggaatcagg
      301 ggaatctgga tgggcggatc gaggttcgaa acatgattgg tcatgaagat gtagctacgc
      361 gagtgatcga actgatcgcg cccgatgatc tcgatcttta cgccggcgag ttgtaccccg
      421 acgtaggctc cccaggtgaa catgcgatag agaaggcgaa tgtcgccggt gagaaatgtc
      481 caggggaagc cgatcaatgc cgcgaaggga gcagccaggc tccagaacgt cagcatgatg
      541 atagtgcgga tcatgggagg ctattgaatc ataaaagcgt agtcatctga tcggcccact
      601 cgaagtctgc gcggcggcct gtgcttcgcc gttcagccac gcggtacggc gggcgcgctg
      661 ctcgggggta acatgatcga ggtcttttac ttcatacgac acttcctggc ggccgccgac
      721 tttccacttc agctcgcgct cgccgttctc gccgcggatt ctcaaagtga gcgcgtcacc
      781 gggtttcagg cgcaggaaat cagggcgtag ttcctgtccc ggcggcttgc cctggatttg
      841 caggacggaa tcgcctacct tcaagccggc tcgctcgcct tcgctgccgg gggtgatggc
      901 gtcgaccgtc attggcgcgt caaaatcgcg ggaggcgctg aagccgagat cggcgacggt
      961 agtggtctcg gttgccaccc gaaggccgac agaacggaaa aaatcgttcc aggggatctc
     1021 ttccttcccg gctacgtatt tggcaaagaa ccatcccagg tcggcgtgag tgacggcctc
     1081 agctgcctgg cgcactcctt cggagtcgtt gaagaaacgg ccctgcttgg cgtagttcgc
     1141 gttcatccac tgcaagactt cgcgcagtga cgcctggcca tggctggcgt cgcgcacagc
     1201 gagatcgagc atcacgccaa gcagttcgcc cttgttgtag taggaaatgc tgcgatcggg
     1261 gcggcggtaa taggaactgc cttcgagcca ggcatcgagg ctcgactctt cggcggattg
     1321 cgtgaggtgc gcgggacggc gctccagttc ggtgatctgc gcgcccaggc ggtacaggta
     1381 aaacccttcg tcgagcaggc ccgcgcgaag ttgaatgatg ctctccgcag tgctggtgac
     1441 gccttcgcag aaccacaagg cacgggtgaa attttcgcgg gtatagtcca ccggctcgag
     1501 cgtttccggg cggatgcgct tgacgttcca aagatgaaag aactcgtggg cggtgatgct
     1561 ggtcagccct tgcaaggtct gccgcatcac gtccgcattg atgtcgatgg ccgtcgaaaa
     1621 cgcgtgctcc ataccgcctc cggcggggcc gtgcgggaag tggtagagga acatgtaggt
     1681 gtcgaagggg cggtcgctca tccaggtagt ggctgcggct acgatcttgt gcagcgcggc
     1741 gacgatcttc tccatgtcgt agtcggaggg gtcggcgtcg acgataacgc ggtagtgtcc
     1801 gccggattcg tcgaaatcgg attcgcggaa atcgctgatc tcgaccgggg agtcgaccag
     1861 acggtcgtat ccggcggcgt cgaagtcaac gccggacacg gacataagcg gagtggccgc
     1921 gcgccatccg gcgggaatgt gatcgaggtg aacgtgcagt ggggagttgc gggcgtcgac
     1981 gggatacatc aggatttgcg cgagattgaa gaaggcgtgg tgaggggtca actcggctgc
     2041 gaaagggccg ggcgtgttta cgaagatctg atattcgacc actgcgccgt tctcggcgcc
     2101 ttgaatttgc cagcggcttt ggttgagttc gcggacggcg agtaccttgc cagagcgatc
     2161 gcgcgcgtgg acccaattca cgtattgcgc gaaattgcga acctgataga gggcgttcca
     2221 gacggggagt tgcaactcgc gctgggttgc gccggcaggc agggcaattt gcacttccac
     2281 aagatgctgg ctgggtgaag caagggagat gttgtatccg acgggcgcct gcgcataggc
     2341 aatcgatgcc gcgagcgcaa cggcgcagag ggcacgccgc agccattgcc ggactggagg
     2401 ctggacggtc acctggtgtg caccagcttc ttgagctgtg ccggcagttt ctcgtagatt
     2461 ccgccgaagc cgccgttgga gaggatggcc acgacgtctc ccgcacgcat ttcgggggct
     2521 acagtctgga caatggcatc ggcatcggcc aaaaggcgcg cgcggcgccc gttgcgttcg
     2581 atctccgccg ccagcgtagg cagttcgagg cgttcgttct cgggaacggc ttcggagcgg
     2641 aacacattag ccacgacaat ttcatccgcc agcgccaggc tgcgggctaa atcgttttgc
     2701 agcacgcgac ggcgcagcgt gttggaacgc ggctcgagaa tggcccacag gcgcgaaccg
     2761 gcatagcgag cacgaagtgc cttcagggtt ccggcaatcg ccgtgggatg atgggcgaag
     2821 tcatcgatga tggtgatgcc gttcacctcg gctttcactt cgagacggcg cttcacactt
     2881 ttgaaggtgc gcagcgcttc tgcgatagca tctttcggca cgccgcagga tgtagccagc
     2941 gccgcggcgg cggtcgcgtt ccagacgtta tactcgccgg ccagggcaaa ctcgaattcc
     3001 gaccacggct ggccgtcatg catgacgctc cacgatgtgc gccctggatc caggctaaga
     3061 ttcgtgactt tccaatgcgc gcgcgacccc gcgccgtagc gctcgaccgg gcagaaggcc
     3121 ttagccagac accgctccag gctgggactt tcggtagctt ccccgacggc gccatcgaag
     3181 gcgatgatcc gtccccggcg tggaaccagg ttcacgagac gcctgtatgc ggtctccacg
     3241 gcgtcgagat ctttgtaaat gtcggcatga tcgaactcga ccgaagtcac aatcaccgcg
     3301 tcaggaaagt agtgcatgaa cttggggccc ttgtcgaaga aggcggtgtc gtattcgtcc
     3361 ccttccagga tgaagtactt gcccttcccg agatgaaagc tggagccgaa gttttcggcg
     3421 atgccgccga tcaagaacga cggttcgagc ccagcggaat ggaagatcca ggagagcatg
     3481 gag
//
LOCUS       DASPUL010000033         3450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_4646, whole genome shotgun sequence.
ACCESSION   DASPUL010000033 DASPUL010000000
VERSION     DASPUL010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4646"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2324)
                     /locus_tag="VEJ00_00730"
     CDS             complement(<1..2324)
                     /locus_tag="VEJ00_00730"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HXW99709.1"
                     /translation="MSTLVDIGTPAARRSPVLRLLLWLSLALVLLLAGVAGYAWFVAE
                     SALPQLDGRLQISGLSAPVTVTRDPHGVPTIDAASLDDLFFAQGYVTAQDRLWQMDVM
                     RRFAGGELSEILGEQTLKVDREQRILGLRAATEKPLAMASVRDRSFLEAYARGVNAYI
                     SSHGTRLPIEFRILGYEPKPWRPEDSLVIANQMVKDLNYHYFFDALAREKILAKLGPE
                     LTADLYVNRSWHDRPPTVMRQDLAEPPNHTGDSDEDDDDDDDDSGPDNAVTQRAVPSS
                     MQEGMPVNGSNDWVISGAHTVTGKPLLSNDMHLGHQMPNLWYEAHLHAGALDVAGVTL
                     PGMPFVVVGHNQRIAWGFTNVGPTVTDVYIENCNAQGACQTPTAWVQPEHRTETIHVK
                     GKPDVTIDVKITRHGPIITALVPGETRPLALRWTLYDGLRIPFFDVNAAQNWEEFRKA
                     FSLLDAPGQNVVYADVDGNIGYQTTGKIPIRAAGDGSLPVSGADNAHEWVSYIPFDKL
                     PSIYNPSSGIIATANARIAPDDYPQSISMEWEAPWRTERIYHLLESGRKFSPSDMLTL
                     QNDIHSEAELFVAERLVYAVDHASKPSPRARQAADLMRNWDGRMLAESAAPTIAESSM
                     RELRRLLLEPKLGRAPADPDNEEEILSWKTYSWEMRSVWLENVLLHHPKRWLPERYPN
                     YDELLAAAVEAAVTGPAAPKDLAQWRWGSFNAVDIEHPILGRIPLLSHWSGPGVQEQS
                     GSAYTVKAVTRHHGPSERFTANMADLDQSTLNVVT"
     gene            2400..2921
                     /locus_tag="VEJ00_00735"
     CDS             2400..2921
                     /locus_tag="VEJ00_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HXW99710.1"
                     /translation="MLCWLRFQSAMLQKRIDDQPTLSRQMVREAQGILHQHLKRVGLK
                     QTAQRDAILRTFLETRDHLSTDELHRLVQKKDARIGYTTVYRTLKLLAECGLASAVAF
                     HDGIARYEHQYQRRSHHHMVCTECGSSVEFFSPEVSQLEQEIGRRYHYLTTRHTFQIY
                     GLCEDCRKAREAS"
     gene            complement(2940..3365)
                     /locus_tag="VEJ00_00740"
     CDS             complement(2940..3365)
                     /locus_tag="VEJ00_00740"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HXW99711.1"
                     /translation="MRYTHWQRLKLALLGAGCLLLALLIIFDPATSGIFPPCPVRYLT
                     GWYCPGCGSLRAIHQLLHGNVQAAWAMNPLTVIFLPFVTYGLASHALMEFRGRGLPQF
                     FIPAIWIRALCILVVLFGVARNLPLHPFSLLAPGVMLRP"
BASE COUNT          672 a         1047 c         1090 g          641 t
ORIGIN      
        1 gtcacgacgt tgagtgtgga ctggtcgagg tctgccatat tcgccgtgaa ccgttccgac
       61 ggtccgtggt gccgggtaac ggccttcacg gtatacgcgc tgcccgactg ctcctgcact
      121 cccggccctg accagtggct gagcagcggg attctcccca ggattgggtg ttcgatgtcg
      181 acggcgttga atgaccccca gcgccattgc gccaggtcct tgggagcggc cgggccggtc
      241 accgcagctt ccactgcggc ggcaagtaat tcgtcgtagt tgggatacct ttcgggcagc
      301 cagcgcttgg gatggtgcag cagaacgttc tccagccaca ccgagcgcat ctcccacgag
      361 taagtcttcc agctcaggat ttcttcttcg ttgtcgggat cggccggtgc cctgcccagt
      421 ttcggctcga gcaggagccg ccgcaactcg cgcatcgaac tctccgcgat ggttggggcc
      481 gccgattcgg ccagcatccg gccgtcccag ttccgcatca ggtccgcagc ctgccgcgcg
      541 cgcggcgaag gtttgctggc gtggtcgaca gcgtagacca ggcgttcggc cacaaacaat
      601 tccgcttctg agtgaatgtc gttttgcaga gtgagcatgt cggagggtga gaactttcgc
      661 cccgactcga gcaggtgata gatgcgctcc gtgcgccagg gagcttccca ctccatgctg
      721 atcgactgcg gatagtcgtc cggtgcaatg cgggcattgg cggttgcgat gattcccgac
      781 gacgggttgt agatgctcgg cagcttatcg aaagggatgt atgagaccca ttcgtgggcg
      841 ttgtccgcgc cgctgaccgg aaggctgccg tcgccggccg cgcggatggg aatcttgccc
      901 gtcgtctgat agccgatgtt tccgtcaaca tcggcgtaga ccacattttg gccgggtgcg
      961 tcgagcagcg aaaaggcctt gcggaattct tcccagttct gggccgcgtt gacatcgaag
     1021 aacggaatgc gcagtccgtc gtagagcgtc cagcgcagcg ccagcggccg ggtttcgccc
     1081 ggaaccaggg cggtgatgat cggcccatgt cgcgtgatct tcacgtcgat ggtcacatcc
     1141 ggcttgcctt tgacgtgaat tgtttccgtg cgatgctctg gctgaaccca cgcggtgggc
     1201 gtctggcagg caccttgcgc gttgcagttt tcgatgtaga catcggtcac cgtcggtccc
     1261 acgttggtga atccccaggc gatgcgctga ttgtggccca cgacaacaaa tggcatgccg
     1321 ggcagcgtga cacccgcaac gtcgagcgcg ccggcgtgca gatgggcctc ataccacagg
     1381 ttcggcatct gatggccgag atgcatgtcg ttcgaaagca gcggcttgcc ggtcacggtg
     1441 tgcgcgccgg aaattaccca gtcgttggaa ccgtttaccg gcatgccctc ctgcatcgaa
     1501 gaagggactg cacgctgcgt gaccgcgttg tccgggccgg aatcgtcgtc atcgtcatcg
     1561 tcgtcctcgt cggaatcgcc cgtgtgattc ggcggctcgg cgagatcctg gcgcatcacg
     1621 gttggcggac gatcgtgcca ggagcggttg acatagaggt ctgcggtcaa ctcgggcccc
     1681 agcttggcca ggattttctc gcgtgccagc gcgtcgaaga agtagtgata gttcagatcc
     1741 ttgaccatct ggttagcgat gaccagcgaa tcctcgggcc gccacggctt gggctcgtag
     1801 cccaggatgc gaaactcgat gggaagcctc gtgccgtggc tgctgatgta ggcattcacg
     1861 ccgcgtgcgt aggcttccaa gaagctgcgg tcgcgcacgc tggccatggc aagtggcttc
     1921 tccgtcgccg ctcgtaggcc caggattctc tgctcgcggt cgaccttgag cgtctgctcg
     1981 ccgagaatct ccgacagctc tccgccggcg aaacgccgca tcacgtccat ctgccacagc
     2041 cggtcttgcg cggtgacgta gccttgggca aagaaaagat cgtccaggct cgcggcgtcg
     2101 atggtgggca ccccgtgggg gtcgcgcgtc acggtgacgg gagccgagag gccggagatc
     2161 tgcaggcggc cgtcaagttg aggcagcgcg gattccgcca cgaaccaggc gtaacccgcc
     2221 acgcccgcga gaagcaacac cagggcaagt gacagccaaa gaagcaggcg gagaactgga
     2281 gatcgtcgtg ccgcgggagt gccgatgtct acgagcgtgc tcatcgagaa tcttcgattg
     2341 taagccaggg caggcagccc gggacgcact gaaaatgaat ttcattttca agatggccga
     2401 tgctatgctg gctgcggttc cagtccgcca tgctgcagaa gcgcatcgat gaccagccca
     2461 cactctcgcg ccagatggtg cgcgaagccc agggcatcct gcaccagcac ctgaagcgcg
     2521 tcggcttgaa gcagacggcg cagcgcgacg ccatcctgcg tacgtttctg gaaacccgcg
     2581 atcatctttc caccgacgaa ctccatcgcc tggtgcagaa aaaagatgcg cgcatcggct
     2641 acaccaccgt ctatcgcacg ttgaaactgc tggctgagtg cggtttggct agcgccgtgg
     2701 cctttcacga cggcatcgcg cgctacgaac accagtacca gcgccgcagc catcaccaca
     2761 tggtttgcac cgagtgcggg agctcggtcg aattcttctc gccggaagtc agccagctcg
     2821 agcaggagat cggccgccgc tatcactacc tcaccacgcg ccacacgttc cagatctacg
     2881 ggctgtgcga agactgccgg aaagcacgcg aagccagcta gtcccggcgt tcatttccgc
     2941 tacggacgca gcatcacgcc cggcgccagc agactgaagg ggtgcaaggg aagatttcgc
     3001 gcaaccccaa acagaaccac gagaatgcag agtgcgcgaa tccaaatggc agggataaag
     3061 aactgcggca atccccgacc gcgaaattcc atcagggcgt gggaggcaag accataggtg
     3121 acgaatggca ggaaaatcac ggtcagcgga ttcatcgccc atgccgcctg cacgtttccg
     3181 tgcagaagct gatgaatggc ccgcagcgag ccgcatcctg ggcagtacca gccggtcaag
     3241 taccgcacgg ggcaaggagg aaaaatcccc gacgtggcgg gatcaaatat gatcagcagg
     3301 gccagcaaga ggcagccggc cccgagcaat gctagcttga gtcgctgcca gtgcgtgtac
     3361 cgcataggtc gaaactccta atggtgggca aggctgccca tgatgcccag tatgacgagc
     3421 agaccccaga gcaggaatcc ggcaaaaaat
//
LOCUS       DASPUL010000034         4346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_279235, whole genome shotgun sequence.
ACCESSION   DASPUL010000034 DASPUL010000000
VERSION     DASPUL010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279235"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1496)
                     /locus_tag="VEJ00_00745"
     CDS             complement(24..1496)
                     /locus_tag="VEJ00_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99712.1"
                     /translation="MSSKSHSTVCNYSAICAWAMATCLLSIPLTTSAQSGQGGSLADT
                     ARQVRAQKAGASDGASRAQQVAAELSDDQNDSAPGGFKTFNAGDYALWVPAPYKVEGN
                     DSAGVVLSGPMMGVKHALVLVGTPMAARWQNDDAAFEETVTKFAHLYSESAHCAKVTI
                     ANHTAYQCGLAAANLLGSQVSGNAVFMMAAGNIYPVFCVSPTDSRSRDLLNNARASSS
                     VKDWSRENLDREEQDTRRVWQQCDSVFQSFRLKPTAQPVAEQASKSSGDPSGTAALTG
                     PGQTAKLVTTTGGAAADGNARAAVVEATARTNQGPVPAAEAQSVSGNVPAPATAPAGF
                     KSQPFKYCKGQSQCWDASFNIPAEAQLISSECGQYTFQIKVQGEPFLLMAGSNGGSCG
                     RAANDANLVRWKDLADPETMRAPGTSSTISSLQGTLSGVPAIITTIGFRKGLVEWMGK
                     RVEVESNGVPLVVGCTATRAHFYDGDAACSALIESLRLQQ"
     gene            complement(1513..2223)
                     /locus_tag="VEJ00_00750"
     CDS             complement(1513..2223)
                     /locus_tag="VEJ00_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99713.1"
                     /translation="MGDRYWLWVGARLLVAVAIVGWAASYIGTGSGEKEFQRTLDAMK
                     QIHSFRAATVASPLTQHNEFLWEVDCGRNIAHYQEHITNQCGDDNTHCSNVDMSREEL
                     DWAVYEYDRQSDGSWQASRHPAGNRTRYLCGHLSDGTDSNLLPPIATMIRRGIIQKGD
                     KKTVNGVRCREYLVTMKGAPGGLEHDTVCLGLEDRLPYEWTVDWEKARTSFSDYNTAI
                     QFDLPAEVVKETSASTSN"
     gene            complement(2468..3292)
                     /gene="amrB"
                     /locus_tag="VEJ00_00755"
     CDS             complement(2468..3292)
                     /gene="amrB"
                     /locus_tag="VEJ00_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HXW99714.1"
                     /translation="MATTTLRHPAVAGRFYPRHAEELRTEALAYLQLAPGQKPAVRAI
                     GCIAPHAGYMYSGHVAGAVFARVEIPRRCVVMCPNHTGMGRALAIISDGAWETPLGEV
                     PVDADLAAALKRHCALLHEDAAAHRAEHAAEVELPFLQLRQPDLKFVPIALGTGQFEL
                     LEPLGKALADVIAKQDDPVLIVASSDMNHYESDAVTRVKDHRAIERILSLDPRGLYDV
                     VTQQEISMCGFGPAVAMLTAARQLGAKSAELVKYATSGDVSGDRDMVVGYAGVVVL"
     gene            complement(3405..4268)
                     /gene="rsmA"
                     /locus_tag="VEJ00_00760"
     CDS             complement(3405..4268)
                     /gene="rsmA"
                     /locus_tag="VEJ00_00760"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXW99715.1"
                     /translation="MPKVASARRPQAKPKLGQHFLASDDWAARIVGALGDISQSAVLE
                     IGPGRGVLTSLLAKRARRLIAVEVDRVLAAQLRLKFGMARNVEILEADILTIDFDSLF
                     GPKPGLGRPGIDIKPEPVRVVGNLPYYITSDILLRLFVFAKYFDTIVIMVQREVADRI
                     AASPGGRDYGMLSATTQLYAHVEKLFTLPPEAFVPPPKVHSTVLRLTMEPRQQELGVA
                     GDGFIDFLRLSFGQKRKTLWNNLKAKYAEPALRRALGDAGVKATARAETLSLEQSAAV
                     YRALREGGERS"
BASE COUNT          761 a         1425 c         1285 g          875 t
ORIGIN      
        1 acgaactgga aacttgctcg tcatcattgc tgcagtcgta gagattcgat cagcgcagaa
       61 caggctgcat cgccgtcgta gaaatgcgcc cgtgtcgccg tgcagcccac gaccagcggc
      121 actccgttgc tttccacctc gacccgcttg cccatccatt cgaccaggcc ttttctgaag
      181 ccgatggtcg taatgatagc cggcacgccg gatagcgttc cctgcaggct gctgattgtg
      241 gaagacgttc ccggggcgcg catcgtttcc gggtccgcca gatctttcca ccggaccagg
      301 ttggcgtcat tcgcagcgcg cccgcagctg cctccgttcg atcctgccat caacaggaat
      361 ggttcaccct ggaccttgat ctggaacgtg tactgcccgc actcggaact gatcagctga
      421 gcttccgcgg gaatgttgaa cgacgcatcc cagcattggc tctggccttt gcagtatttg
      481 aacggttggg acttgaatcc ggccggcgcc gtagccggcg ctggcacatt gccggaaacc
      541 gattgggctt ccgccgccgg caccgggccc tggttggtcc gcgccgtggc ttccaccacc
      601 gcggcgcgcg cgtttccgtc cgccgccgcg ccgccggtag tcgtcaccag tttcgccgtc
      661 tggcccgggc cggtcagcgc tgccgttccc gacggatcgc cggagctctt gctggcttgt
      721 tccgccaccg gttgagccgt cggcttcaga cgaaacgact ggaacaccga atcgcactgc
      781 tgccacaccc tcctcgtatc ctgctcctct cgatccaggt tttctcgcga ccagtctttg
      841 acactcgacg acgcccgcgc attattgagc agatcgcggg agcgactgtc ggtggggctg
      901 acgcagaaca cgggatagat gttgcccgcg gccatcataa acaccgcatt gccgctcacc
      961 tggcttccca acaggttcgc cgccgccagt ccgcactggt aggccgtgtg attggcgatg
     1021 gtgacctttg cgcaatgcgc cgactccgaa tacaagtggg caaacttggt gacggtctcc
     1081 tcaaacgcgg catcgtcatt ctgccagcgc gccgccatag gagttcctac cagtactaag
     1141 gcatgcttga cccccatcat cggaccggaa agcacgacgc ccgcgctgtc gtttccttcc
     1201 accttgtacg gagcggggac ccaaagcgcg tagtcgccgg cattgaaagt cttgaagcct
     1261 ccgggtgcgc tgtcgttctg atcgtccgac aattcggcgg caacctgctg tgcgcggctg
     1321 gcaccgtcgg aggcccctgc cttctgggcc cgcacctgtc gcgccgtgtc tgccagcgac
     1381 ccgccctgcc cggactgggc cgaggttgta agcgggatcg aaagcaggca ggtagccatc
     1441 gcccacgcgc aaatcgcaga ataattgcaa acggttgaat gtgacttcga gctcatgggt
     1501 ttcgggtctc cttcagttcg atgtgcttgc gctcgtttcc ttgaccactt ctgcgggcag
     1561 gtcgaattga atcgcggtgt tgtagtctga gaacgaggtg cgcgcctttt cccagtccac
     1621 ggtccactcg tagggaagac gatcctcaag gcccagacag accgtgtcgt gctccagtcc
     1681 acctggagcg cccttcatgg taacgaggta ttcccggcag cgaactccat tcacggtctt
     1741 cttgtcgccc ttctgaatga tgccgcggcg gatcatggtg gcgatcggcg gcagcaggtt
     1801 gctgtccgtg ccgtcggaga ggtgtccgca gagataccgg gtgcgattgc ctgccggatg
     1861 ccggctggcc tgccaggagc cgtcggattg gcgatcgtat tcgtagaccg cccagtccag
     1921 ttcttcccgg ctcatgtcga cgttcgaaca gtgcgtgttg tcgtcgccgc actggttggt
     1981 gatgtgttcc tggtagtgcg cgatgttacg gccgcagtcc acttcccaga gaaattcgtt
     2041 gtgttgggtc agggggctgg cgacggttgc ggcgcggaag ctgtggatct gcttcatcgc
     2101 gtcaagagtt ctttggaatt ccttctcgcc gctacccgtt cctatatagc tggccgccca
     2161 acccacgatg gcgacagcca cgagcaggcg agcccccacc cacagccagt accggtcgcc
     2221 catagacagt cctccccaag cacggtctct cggttaggtt agccgccgga ccggggtgca
     2281 gaaagttacc tccgtacttg aacacatgcc gcggctagca cgggatttcc gttagttgca
     2341 ggtgttttct ggaatcggct ggaggtttcc tgagagagat tcgtcctcgc gctctccgcg
     2401 agttcccgtg gcgtacttga atagcttttt tcgagctgat gatcgaatcc cgacgccggc
     2461 gcccggctca aagcacgact acacccgcat atcccaccac catgtcccgg tcgccggaga
     2521 cgtcgcctga ggtagcgtac ttcaccagtt ccgccgattt cgcacccaat tgccgagccg
     2581 cagtcagcat cgccactgcc ggcccgaagc cgcacatgct gatctcctgc tgcgtgacga
     2641 catcgtagag tccgcgcgga tcgagcgaca gaatgcgctc aatcgcgcga tgatctttca
     2701 cccgggtcac ggcgtccgac tcatagtgat tcatatcgct cgaggcgacg atgagcaccg
     2761 gatcatcctg cttcgcgatg acatccgcca acgccttccc cagcggttcc agaagctcaa
     2821 actgcccggt ccccagcgcg atgggcacga acttaagatc gggctggcgc agttgcagga
     2881 agggaagctc gacctcggca gcgtgttcgg cacggtgggc tgctgcatcc tcgtgcagga
     2941 gcgcgcagtg ccgtttcaaa gctgcagcca gatcggcatc gaccggaacc tcgcccagcg
     3001 gcgtctccca ggccccgtca ctgatgatcg ccagcgcgcg ccccatgccc gtgtggttgg
     3061 gacacatcac cacgcagcgg cggggaatct ccacccgcgc gaagacggct ccggccacgt
     3121 ggcccgagta catgtagccg gcatggggag cgatgcagcc gatggcccgc accgcgggct
     3181 tctgcccggg cgcaagttgg agataggcga gtgcttctgt gcgcaactct tcagcatgac
     3241 gtggataaaa tcgtcccgcg accgcgggat gacgaagggt ggtggtagcc ataggccctc
     3301 ctttcggtta gacccatgta gggacagccg ccctcggctg tcccggtcga gcgcagctcg
     3361 acatcgtcgt gctaacgctg actcattacc tctttgacat gatgctacga gcgctccccg
     3421 ccttcgcgca aggcgcgata gactgccgcg ctctgttcca gactcagggt ctcggcccgc
     3481 gccgtcgcct ttaccccggc gtcacctagc gcgcgtcgca aggcaggctc cgcatatttt
     3541 gctttaaggt tattccacag cgtcttgcgc ttctgtccaa acgaaaggcg caggaagtcg
     3601 atgaacccgt cgcctgccac gcccagctcc tgttgcctgg gctccatggt aagccgaagg
     3661 accgtcgagt gcactttggg cggaggcacg aacgcctccg gcggcagcgt gaacagcttc
     3721 tcgacgtggg cgtacagttg cgtcgtcgcc gagagcattc cgtagtcgcg cccgcccggc
     3781 gaggccgcga tccggtccgc cacttcccgc tgcaccatga tcacgatcgt gtcgaagtac
     3841 ttcgcgaaca caaacaaccg caacaagata tcggacgtaa tgtagtaggg aagattgccc
     3901 acaaccctga ccggctcggg cttgatgtct atgccgggcc gccccagccc gggcttcggg
     3961 ccgaagagtg aatcgaaatc gatggtcaga atgtcggcct cgagaatctc cacattccgc
     4021 gccatgccga acttcaagcg cagctgcgcc gccagcaccc ggtccacttc cactgcgatc
     4081 agccgtcgcg cccgtttggc tagcagggaa gttagaacgc cccggcctgg tccgatttcg
     4141 agcaccgcac tctgtgaaat gtcgcccagc gcgccgacga tgcgcgcggc ccagtcgtcg
     4201 ctggccagaa aatgctgtcc caacttgggc tttgcctgcg gacgccgggc agacgctacc
     4261 tttggcacgt tgaccctcac gcactcggga agtagacttg ccagtcggtc cccggctttt
     4321 tgcctgaggg actccggccc cgagac
//
LOCUS       DASPUL010000035         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_827827, whole genome shotgun sequence.
ACCESSION   DASPUL010000035 DASPUL010000000
VERSION     DASPUL010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827827"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VEJ00_00765"
     CDS             <1..628
                     /locus_tag="VEJ00_00765"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99716.1"
                     /translation="PLMDGPLNPHDILAVLGEKELQAYLVNEIQEVYRLQGVNISDKH
                     IEVIVRQMMRWVKVEDVGDTQFLLEQQLDKFRFREENERVIAQGGKPATGRPLLLGIT
                     KASLSTDSFISAASFQETTRVLTEASIQGAVDHLRGLKENVIVGRLIPAGTGMEYYRN
                     VRLSPEMEEAAAKVQAEVSAAYEEAERALELMRHEGENEELPAEIPAE"
     gene            850..1725
                     /locus_tag="VEJ00_00770"
     CDS             850..1725
                     /locus_tag="VEJ00_00770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXW99717.1"
                     /translation="MLRFLRLFRLSVWRAFVHDAFAIAKASAYSMILTFFPVLLIVGS
                     GLANWRKGAPYLREITYALGSILPAGSNTALTYLKGAAQHPVGLLTTTSLITLWTASG
                     VMISWMDGFRRCYELPKTWGLVKERLIAFGLVIFALVPMTFATLLVALGSKIETRILF
                     YVDPDFSAYIFLLWGAGRWLISTLTSISVIALIYHNAVPRTQPWHSVIPGATLATVLW
                     FSVTVGFRSYLQHFGNFATIYGSLGVAVALLVWMYLISLVVLVGAEFNALLFPRAMLG
                     KELKELTAINMTKAD"
     gene            1736..1960
                     /locus_tag="VEJ00_00775"
     CDS             1736..1960
                     /locus_tag="VEJ00_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99718.1"
                     /translation="MTIAQLRGLQKCTVVLRLTGGEVLMSRINFVDLEYEDIVIDVLE
                     TNRPEQYKGPKNSAYAIKAEDIASIERIMA"
     gene            2229..>2543
                     /locus_tag="VEJ00_00780"
     CDS             2229..>2543
                     /locus_tag="VEJ00_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HXW99719.1"
                     /translation="MPSKLPTSDLEPSPRRAKIICTIGPACHSEALMRDLLRLGMDVA
                     RLNFSHGTHEDHAHNIQRLRRAAEREHRTVCILQDLQGPKIRTGTLEGHEPVLLKTGS
                     VVT"
BASE COUNT          507 a          845 c          705 g          486 t
ORIGIN      
        1 gcctctaatg gacggtccgc ttaacccgca cgacatcctg gccgtcctcg gcgagaaaga
       61 gctgcaggct tacctggtca acgaaatcca ggaagtctac cgcctgcagg gcgtcaacat
      121 ctccgataag cacatcgagg tgattgtccg ccagatgatg cgctgggtga aggtcgaaga
      181 cgtcggcgat acacagttct tgctcgaaca gcaactcgac aagttccgct tccgcgaaga
      241 gaacgagcgc gtgattgcgc aggggggcaa gccggctacc ggccgtccgc tgctgctggg
      301 catcacgaaa gcgtcgctct ccaccgactc gttcatctcc gcagcgtcgt tccaggagac
      361 cacgcgcgtg ctcaccgaag cctccatcca gggcgcggtc gatcacctgc gcggcctgaa
      421 ggagaacgtc atcgtcggtc gcctcatccc tgccggcacc ggcatggagt actaccgcaa
      481 cgtgcgcctc tcgcccgaaa tggaagaggc cgcggccaaa gtccaggccg aagtttcagc
      541 tgcttacgaa gaagctgaac gcgccctgga gctcatgcgg cacgaaggcg agaacgagga
      601 actgccggca gaaatacccg cggaataaga aaacgaaaac aaacctcggg ggcgaagcga
      661 ttcgcccccg aagctgcttt tggagagaaa aaaccaggtt tctgggtgcc ccattctaag
      721 cgcgcttttt gcgcttagag tggggacttt caaaaatcca tggccgcccc ccagcgagtg
      781 cgtatcgagg aacctgcctc acttccttgt ttgcgtccgg tgcctcccgg ccctaatcta
      841 gggcaacgca tgctgcgctt cttacgcctg ttccggcttt cggtctggcg tgccttcgtg
      901 cacgatgcct tcgctatcgc caaggcctcg gcgtattcca tgattctgac gttcttcccg
      961 gttctgctga tcgtcggctc cggcctggcg aactggcgta aaggtgcgcc ctatctgcgg
     1021 gagattacct atgccctggg cagcattctc cccgccggca gcaacacggc gctgacctac
     1081 ctgaaaggag cagcgcagca tcccgtcggc ctgctgacga caacctcgct catcacgctc
     1141 tggacggctt ccggcgtcat gatctcctgg atggatggct tccgccgctg ctacgagctg
     1201 cccaagacct ggggcctggt gaaagagcgc ctgatcgcat ttgggcttgt gattttcgcg
     1261 ctcgtgccca tgacgttcgc cacgctcctc gtcgctctcg gcagcaagat cgagacgcgc
     1321 atcctgttct acgtcgatcc cgacttcagc gcctatatct ttttgctatg gggagcaggc
     1381 cgctggctga tctccacgct caccagcatc tcggtcatcg cgctgatcta tcacaacgcc
     1441 gtgccgcgca cccagccctg gcatagcgtg atccccggcg caacactcgc tactgttctg
     1501 tggttctccg tcacggtcgg attccgctct tatctgcagc atttcggcaa cttcgccacc
     1561 atctacggct cgctgggagt cgccgtcgcc ctgctggtct ggatgtacct gatctcgctc
     1621 gtcgtcctgg tcggcgccga attcaacgct ctgctgttcc cccgcgccat gctggggaaa
     1681 gaactcaaag aactgaccgc gatcaacatg acgaaagccg attagaggaa ttgtggtgac
     1741 aatcgcccaa ctccgggggc ttcagaaatg taccgtggtt ctgcgcctga ctggcggcga
     1801 agtcctgatg tccaggatca actttgttga cctggaatac gaggacatcg tcatcgacgt
     1861 gttggaaaca aataggccgg agcaatacaa gggacccaag aactccgcct acgcgataaa
     1921 ggcggaggac atcgcctcaa ttgagagaat aatggcctga aagcgggccg ccccacgtct
     1981 ctcccctcgg atcaaccgtg gaagagcggc gctttagcgc cgcgcaagcg gtcggtgaga
     2041 atgggcttta gccctggtgg acgcttttcg cgacaacggc caccagccct gaagggcatc
     2101 gtgccctcga tcctaacgcg gccctgaagg gccgctcttc cacgccgacg aacggaaaag
     2161 gacgacccag accgcaaaac gaagccaacg ccccaacccc caccgcattt gctactctgt
     2221 ttggacctat gccgtcgaag ctccccactt ccgatctaga accgtcgccc cgccgcgcca
     2281 agatcatctg caccatcggc ccggcctgcc actccgaggc cctcatgcgc gacctgcttc
     2341 gcctgggcat ggatgtcgcc cgcctcaact tctcccatgg cacacacgaa gaccacgccc
     2401 ataacatcca gcgcctgcgc cgtgcggccg agcgcgaaca ccgcaccgtc tgcatcttgc
     2461 aggacctgca gggcccgaaa atccgcaccg gcacgctcga gggccacgag ccggtcctgc
     2521 tgaaaaccgg ctcggtcgtc acc
//
LOCUS       DASPUL010000036         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_142356, whole genome shotgun sequence.
ACCESSION   DASPUL010000036 DASPUL010000000
VERSION     DASPUL010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142356"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..858
                     /locus_tag="VEJ00_00785"
     CDS             133..858
                     /locus_tag="VEJ00_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HXW99720.1"
                     /translation="MNPIFTSVLLGLTAAAANVFGGAIIVQRHWERRYLRYFVALGAG
                     FMLATATVEMIPESLELRGRSAAFLVLLGYLIIHFFEHTVTPHFHFGEETHHDEFTHA
                     RKSYSVLLGLIIHTFFDGIAIASGFLVSNWLGWLIFLAIFLHKIPEGFTVSSVMLASG
                     RSRGTAWGASMILGGSTLAGVLTMALFRHQVSAGLPLSAGVTIYVAASDLVPEVNKEP
                     AMKMALLVFVGAGVFFLLDRLFH"
     gene            complement(877..2085)
                     /locus_tag="VEJ00_00790"
     CDS             complement(877..2085)
                     /locus_tag="VEJ00_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXW99721.1"
                     /translation="MLCLALICGFLLCSSPTWAQDQQNQQAPPANQTPATAQAPDNGK
                     PKQDVPAEAGGPGDNVGPYVIPKKTANEAPPPPPPPAPKKVADMPDYSLKVNVPLVNV
                     DVLVTTKDGQFVPGLKEENFRVSEDGVPQKVSKFAVSEAPVTAVLLVEFASTSWPILV
                     DTLNASYAFANTLKKDDWVAVAYYDMQPHILTDFTQDKRAVFGALNQLRLPGFAETNE
                     FDALYDTLDRLDRVEGKKYVILVSTGVDTFSKLTLDKITKKIKDTKDVTIFPVSIGWA
                     LREYCEVNHCTGISHGMASYGIHQIDYLQADNEMKTFAAMTGGRAYFPRFQAEFGEIF
                     RDIGDDIRHQYTLAYSPTNTKLDGTYRKLKVQVVAADGGPLKVKDQKGKDVKIEVVAR
                     EGYTAKHTVD"
     gene            2318..>2832
                     /locus_tag="VEJ00_00795"
     CDS             2318..>2832
                     /locus_tag="VEJ00_00795"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HXW99722.1"
                     /translation="MRKFLLALVFVLSPYIFVAARSKPSQPTISVDQIRAGMRGVAYT
                     VFEGTKPEPMEVEVLGVLHNVNGPKGDIILIRLHGPKVEYTGVVAGMSGSPVYLDGKL
                     AGALAFRIGEFSKEPIAGVTPIADMLEINALDKSPAEEAAATKAHPANATGKTAAPGD
                     SSSLPSSTQDFA"
BASE COUNT          512 a          834 c          823 g          663 t
ORIGIN      
        1 cactccggct gatacatccg ccacggaggc agggagacac cgagacaaca atgcatccga
       61 tttctccgtg tccccgtgac tccgtgtctc cgtggttagt ttcggtgtgg ggctgatatc
      121 atcccgttgc tcatgaaccc aatcttcacc agcgtcctgc tgggtctaac cgcggccgcg
      181 gccaatgttt tcggcggagc catcatcgta cagcggcatt gggagcggcg gtatctgcgc
      241 tattttgtcg cgctgggtgc gggattcatg cttgcgaccg ccacagtcga gatgatcccg
      301 gaaagcctcg agttacgcgg ccgttcggct gcatttctcg tgctgctcgg gtatctgatc
      361 attcatttct tcgaacatac agttacgcca cactttcact tcggcgagga gacgcatcac
      421 gacgaattca cgcacgcgcg caagagctac tccgtgctgc tcgggctgat tatccacact
      481 tttttcgacg gcatcgctat cgcttccgga ttcctggttt caaactggct gggctggctc
      541 atttttctgg ccatctttct gcacaagatt cccgagggct tcaccgtaag ttcggtcatg
      601 ctggccagcg ggcgcagccg tggcaccgcc tggggcgctt ccatgattct cggtgggtcg
      661 acgctggctg gagttttgac gatggcactg ttccgtcatc aggtgagcgc gggcctgccg
      721 ctgtcggcgg gggtgacgat ctatgtcgcc gcctccgatc tggtcccgga ggtcaacaag
      781 gaacccgcca tgaagatggc tctgctggtg tttgtcggcg caggggtctt ttttctgctg
      841 gaccgcctgt ttcactgacc gttgtccgtc tgccggtcag tcaaccgtgt gctttgccgt
      901 gtacccttcg cgcgcgacca cttcgatctt gacgtccttg cccttctggt ctttgacctt
      961 cagcgggccg ccgtcggccg cgactacttg caccttcagc ttgcggtaag tgccgtcgag
     1021 cttggtgttc gtggggctgt aggccagcgt gtactggtgc cggatgtcgt cgccgatgtc
     1081 acggaaaatc tcgccaaact ccgcctggaa ccgtgggaaa taggcccgcc ctccggtcat
     1141 tgccgcaaac gtcttcattt cgttgtcagc ctgcagataa tcaatctgat ggatgccgta
     1201 gctggccatt ccatggctta tgcctgtgca gtggttcacc tcgcagtact cgcgcagggc
     1261 ccagccgatg ctgaccggaa aaatcgtgac atccttcgtg tctttgattt tcttggtgat
     1321 cttgtccagg gtcagcttgc tgaaggtgtc aacgccggtg gaaaccagaa tcacgtactt
     1381 ctttccctcg acccgatcga gccgatcgag cgtatcgtac agcgcgtcga attcattggt
     1441 ttcggcaaac ccgggaagac gcagctgatt gagggcgcca aacacggccc gcttgtcctg
     1501 cgtaaaatcc gtcaggatgt ggggctgcat atcgtagtaa gccaccgcaa cccaatcgtc
     1561 cttcttcaac gtattggcaa acgcatagga tgcattcaga gtgtccacaa gaatcggcca
     1621 gctggtcgac gcaaattcga ccaacaagac tgctgtgacg ggagcttccg atacggcgaa
     1681 cttgctgacc ttctggggaa cgccgtcctc cgaaacccgg aagttttctt ccttcagccc
     1741 gggaacgaac tgcccgtctt tggtcgtgac cagcacgtcc acattcacca gcggcacatt
     1801 caccttgagt gaatagtcgg gcatgtccgc gactttcttc ggggcaggcg gtggaggcgg
     1861 gggaggagct tcgttggccg ttttcttcgg gatgacataa ggccctacgt tgtcgccggg
     1921 gccgccggct tcggccggca cgtcctgttt gggcttacca ttgtccggcg cctgggcggt
     1981 ggcgggagtc tggtttgcag gcggagcctg ctggttttgc tggtcttgcg cccatgtcgg
     2041 cgaactgcac agcagaaatc cgcatatcag ggccaggcag agcatcaggg ggagcaaact
     2101 cccgggccga gtcacagaat tgcgctgcaa gatcttattg ttctgtcgat tacacatagg
     2161 ggaacccctg aaaaagcctc tatgctagta tagacgctag gattcgcaat tgggatgttt
     2221 tgcccggttt ctcggctccc ctcctggcaa caagtcacag tttgtacgag tctaaggtag
     2281 taatgggacc tgttggcggg tcctcccggg gctttacatg aggaaattcc ttctcgcgct
     2341 cgtcttcgtc ctgtctccct acatttttgt ggctgcgcga agcaagccgt cacagcccac
     2401 catttccgtg gatcaaatcc gcgccggcat gcgcggtgtg gcttatacgg tctttgaggg
     2461 aacgaagccc gagcccatgg aggttgaagt tctcggcgtg ctccacaacg tgaacggacc
     2521 caagggcgac atcattctga tccggctgca cgggccgaag gtggagtaca ccggcgtcgt
     2581 cgccggcatg agcggaagtc ccgtctatct cgacggcaaa ctggcgggcg cgctcgcgtt
     2641 ccgcattgga gagttttcca aagagccgat cgccggagtc acgcccattg ccgacatgct
     2701 cgagatcaac gcgctcgata agtcgccggc ggaagaagct gctgccacga aggctcaccc
     2761 agcgaacgcc accggcaaga ccgcggctcc cggtgactca agttcgctcc caagttcgac
     2821 tcaagacttc gc
//
LOCUS       DASPUL010000037         5201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1924524, whole genome shotgun sequence.
ACCESSION   DASPUL010000037 DASPUL010000000
VERSION     DASPUL010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1924524"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..249)
                     /locus_tag="VEJ00_00800"
     CDS             complement(19..249)
                     /locus_tag="VEJ00_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99723.1"
                     /translation="MSKMIQLRNVPDALHRNLKARAAMAGMSLSDYLLGEIKEIAERP
                     TLAELRERLHRREAVSVPLDSVRLVREERDAR"
     gene            complement(462..1523)
                     /locus_tag="VEJ00_00805"
     CDS             complement(462..1523)
                     /locus_tag="VEJ00_00805"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXW99724.1"
                     /translation="MTTSSMNTLVQPLGEMPPESIVFGRTEGMQAVRDRLVKLASANV
                     PVLIQGESGTGKDIIARMIHAASPWRSGPWVKVNCPAIPGTLLESELFGYEKGAFTGA
                     YGMKPGRVEMAHRGTLFLDEISELDMPLQSKLLQLLQDGQFCRIGAQEDKKVEVRVVC
                     ATNRKLEQEIENGTFRSDLFYRINVVNLHLPPLRDRVIDIPELVTYFLDYHNRKYNCR
                     ARMLSNEMMSVLRKYHWPGNIRELENLIKRYVILGNEEVISADLAPRNADFFNAEIPL
                     DGQISLKKLTRQAVRELERKVILKVLQNHHWNRKQAARALSISYRALLYKIRDAGLPS
                     NRTVKQREPEPAKQEVAAD"
     gene            complement(1720..2100)
                     /locus_tag="VEJ00_00810"
     CDS             complement(1720..2100)
                     /locus_tag="VEJ00_00810"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXW99725.1"
                     /translation="MHVARPGTERRKWERLPLAIPVFVRSQDEKGKEFLEFATALNVS
                     AGGMLVAVRRVLPVVAELQLEIPSPPVAALALRPRASRNLRAKALRSTPAEGYYLLGI
                     KFSRPLLRSGRSNSRPKRKILSAV"
     gene            2455..3387
                     /locus_tag="VEJ00_00815"
     CDS             2455..3387
                     /locus_tag="VEJ00_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXW99726.1"
                     /translation="MATAQKNTCVPDALGAASGPDPAADAGGHSAADVPSPENLLQEY
                     TELRERFRRTTNALASAAHDLKTPLSILNGYVELLQSEKLGPVTDRQREVLHDMQANG
                     KRLQQYIQDFLTFSALETGGLRMHFDQGNINDCLSGVCKLWSGRFQEKGIALYFLAND
                     KLPVFPFDAPKLERVISNLLENSFKFTPQGGTVWLHAEPHMWDRRTAAVPSAAERRRQ
                     NLSHPNAVKVSVSDSGPGIPAEFHLEVFDDFFRLPGTESQEGMGLGLAIARRLVQGMG
                     GKIWVESETGAGCKFSFLVPYKPAAAAASKGKNR"
     gene            3384..4790
                     /locus_tag="VEJ00_00820"
     CDS             3384..4790
                     /locus_tag="VEJ00_00820"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXW99727.1"
                     /translation="MKPTAKILLVDDEPGMLRYIKTLLEVDDYKVETATTGEEALERV
                     EKGLQPDLVLLDVIMPGIDGLQTLERLRQMHAGVKVVMLSCVNDTRKVVQAMKLGAQD
                     YLTKPFQKAELDAVIDLSIGQGQKNLGGEVEELSEDTFFVAASPAMRKIRSQAALVAN
                     VDIPVLLLGESGTGKEVLARLIHKLSPRAHRTFLKVNCAAVPADLLESELFGYEPGAF
                     TGATHAKPGKFELCNKGTILLDEIGEMPPLLQAKLLHVLQDQQFSRLGSRSVIKVDVR
                     ILAATNINIPEALATKRLREDLYYRLNAFTLHIPPLRERKEEIPILLKHSMSRMAERY
                     ARPPLPLSPSLLQACQDHSWPGNLRELNNFLKRYLILGDESLALTELQPKDDGGGGMR
                     SNSAARGSEPGGLKSLARSAKDEAEAQAITQALEQTNWNRKQAAAILQISYKALLYKI
                     RQYGIAEAKSHHKLSAGA"
     gene            complement(4986..>5201)
                     /locus_tag="VEJ00_00825"
     CDS             complement(4986..>5201)
                     /locus_tag="VEJ00_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99728.1"
                     /translation="LGLGTQDRRRRRLMGIIALCALSFLLLLLPACSHQTTQIPPSGT
                     PPGTYTITITATSGSDTKSTSVTLIVP"
BASE COUNT         1060 a         1542 c         1616 g          983 t
ORIGIN      
        1 ccgaggcgtc cagcacaatc accgcgcgtc gcgctcctcg cgcacgagtc ggaccgaatc
       61 gagcgggacc gacacagcct cgcgccggtg cagccgctcc cgcagctccg ccagagtggg
      121 ccgctccgca atctctttga tctcccccaa cagatagtcc gacagcgaca tgcccgccat
      181 cgcagcgcgc gccttcagat tgcgatgcag ggcatctggc acattgcgca gctggatcat
      241 tttggacatg ttctaagcat actttcatgt gttacacatg tcaattagcc gcacgacacc
      301 cctcgtggcc tgaaagaagg tcgaccgatc gataacctac acgtcctcag gaacttgcgg
      361 cccgcaaaac tcctgaactg tcttttgtcg gttagtgttg cggacaggaa ccagcactag
      421 cttccaataa ctcggttaac ggttagaaat gcgccaattc tttagtcggc cgccacttcc
      481 tgctttgccg gttccggctc tcgctgtttg accgtgcggt tcgagggcag gccggcgtcc
      541 cgaatcttgt aaagcagcgc gcggtagctg atgctcaacg cgcgtgccgc ctgcttgcgg
      601 ttccagtggt ggttctgcag aactttcagt atgactttcc gttccagttc gcgtaccgcc
      661 tggcgggtaa gcttcttcag cgaaatctgt ccgtcgaggg gaatctcggc gttgaagaag
      721 tccgcatttc tcggagcgag atcggccgag attacttcct cgttgcccag aatcacgtag
      781 cgcttgatca ggttctcgag ctcccggata ttgcccggcc agtggtactt gcgcagcaca
      841 ctcatcatct cgttcgaaag catgcgggcg cggcagttgt acttgcggtt gtgatagtcg
      901 aggaaatagg tgaccagttc gggaatatcg atcacgcgat cgcgcagcgg cggcaggtgc
      961 aagtttacga cgttgatgcg gtagaacaaa tcgctgcgga aagtgccgtt ttcaatctcc
     1021 tgctcgagct tgcggttggt ggcgcagacc acgcgcactt cgactttctt gtcttcctgg
     1081 gcgccaatgc ggcagaactg gccgtcctgc agaagctgca gcagcttcga ctgcaagggc
     1141 atgtcgagct ctgaaatctc atcgaggaac agcgtgccgc ggtgcgccat ctccacccgg
     1201 ccgggcttca tgccgtaggc cccggtgaag gctccttttt cgtagccgaa gagctcactt
     1261 tcgagcagcg tgccggggat ggccggacag ttgaccttca cccaggggcc gctgcgccag
     1321 ggcgaggcgg cgtggatcat gcgggcgatg atgtctttgc cagttccgct ttcaccttga
     1381 atgaggacag ggacgttggc gctggccagt ttgaccagcc gatcgcgaac ggcttgcatg
     1441 ccttcggtgc gcccaaagac gatcgattcg ggcggcatct cccccagagg ctgcacgaga
     1501 gtgttcatgc tgctggtggt catggacggt ttccggtggg gggatagcct tgagctagca
     1561 cccggagaac caaaacgggg caagctcatc gcggtccctg caaatctaac ctatgtgatt
     1621 gatgagtagc aacttaccaa agagacacca aagcgcaccg gggtcatccg gaggcgggaa
     1681 caacagtaac accgacgggc taagcgaggg aaaacatttt cacacagcag aaagtatttt
     1741 ccgcttgggc ctgctattgg agcggccgga gcgcagtaag ggacgtgaga atttgatccc
     1801 cagaaggtag taaccctcgg cgggagtact acgaagggcc ttggcgcgca ggtttcgcga
     1861 agcgcgcggc cgcagcgcca gagcggcgac gggcgggctg ggaatctcca actggagctc
     1921 ggctacgacg ggcaggacgc ggcgaacggc gaccagcata cctccggcgc tgacgttgag
     1981 ggcagtggca aactccagga attccttgcc cttctcgtcc tggctgcgga caaagaccgg
     2041 gatggccagc ggcagccttt cccacttgcg ccgctcggtt ccgggccttg ccacgtgcat
     2101 gcagagctct cccagtacct cagaagatta gatttcaagc accttagggg ccaaatctcg
     2161 ggcaaatgcc ggaacaagcc gggtcagaac cgccgctctc gggcactcca gaggcaagaa
     2221 ggggcattgg aaccggagtt tggcccggtt ctgcctaagt tggaggctgc gcgggacggg
     2281 cgcggcgaag cccgcaggca tactcggaag tgcaagattt tgccacactt gggtaattcg
     2341 cttgcatttt tcgttgacaa ccccataggg ctgcaatagg atttttctat tcggccctga
     2401 ctaatttgcg gtggggctgt ttcccttccc cagctgtcac ctgtggtttt cccaatggcc
     2461 actgctcaga agaatacgtg cgttccagac gccctcggag ccgcctcagg accagatccg
     2521 gcggcagatg cagggggcca ctcggcagcg gacgttccga gcccggaaaa tctgttgcag
     2581 gagtacaccg aattgcgcga gcgctttcgc cgcaccacca acgcgctggc atcggcagcc
     2641 cacgatctca agacgccgct ttcgattctc aatggctatg tggaacttct gcagagcgaa
     2701 aagctgggtc cggtcaccga tcgtcagcgt gaagtactgc acgacatgca ggcgaacggg
     2761 aaacgtctgc agcagtacat tcaggacttt ctgaccttca gcgcgctcga aaccggcggg
     2821 ctgcgcatgc acttcgacca gggaaacatc aacgactgtc tttcgggcgt gtgcaaattg
     2881 tggtcgggcc gctttcagga aaaaggcatc gcgctctatt tcctggccaa cgacaagctt
     2941 cctgtgtttc ccttcgacgc tcccaagctg gagcgcgtga tttcgaacct gctggaaaat
     3001 tctttcaagt tcacgccgca gggtggaacg gtatggctgc acgccgagcc gcacatgtgg
     3061 gaccgtcgca cggctgccgt gccgtcggcg gccgagcgcc ggcgccagaa cctgagccat
     3121 cccaacgcgg tcaaggtgag cgtttccgac tccggcccgg gaatcccggc ggagtttcat
     3181 ctggaagtgt tcgacgactt tttccgcctg cccggcaccg aatcccagga aggcatggga
     3241 ttgggactgg ccatcgcgcg ccgcctggtg cagggcatgg gcggcaagat ctgggtggag
     3301 agcgaaacag gcgcgggctg caagttctcg tttctggtgc catacaaacc ggccgctgcc
     3361 gccgccagca aaggaaagaa tcgatgaaac caaccgccaa gatcctgctg gttgacgatg
     3421 agccggggat gctccgctac atcaagacgc tgctcgaggt cgacgactac aaagtcgaga
     3481 ctgcgaccac gggcgaggaa gcgctggagc gcgtagaaaa gggcttgcag cctgatctgg
     3541 tgctactgga cgtgatcatg cccggcatcg acggcctgca gacgctggaa cggctgcgcc
     3601 agatgcacgc gggcgtgaag gtggtcatgc tgtcgtgcgt caacgatacg cgcaaagtcg
     3661 tgcaggcgat gaaactgggc gcacaggact acctgaccaa gccctttcaa aaggccgaac
     3721 tcgacgcggt gatcgatctc agcatcggtc agggacaaaa gaatctcggc ggcgaagtgg
     3781 aagagttgtc cgaagacaca ttcttcgtgg ccgccagccc ggcgatgcgc aagatccgct
     3841 cgcaagcggc gctggtggcc aatgtcgata tcccggtgct gctgctgggc gagagcggaa
     3901 ccggcaagga agtgctggcg cggctgattc acaagctctc gccgcgggcg catcggactt
     3961 tcctgaaagt gaattgcgcc gccgttcccg ccgacttgct ggagagcgaa ctgttcggct
     4021 acgaacccgg agcgttcacc ggcgcaaccc acgccaagcc gggcaagttc gagctgtgca
     4081 ataagggcac aatcctgctc gatgagatcg gcgagatgcc gccgctgctt caggcaaaat
     4141 tgctccacgt cttgcaagat cagcagttct cgcggctggg aagccgctcg gtgatcaagg
     4201 tggatgtgcg catcctggcg gccaccaaca tcaacattcc ggaagcgctg gcgaccaagc
     4261 ggctgcgcga ggatctttac taccggctga atgcgttcac gttgcacatt ccgccgctgc
     4321 gcgagcgcaa ggaagagatt ccgattctgc tgaagcactc gatgtcgcgc atggcggagc
     4381 gttatgcgcg tccgccgctg cccctgtccc cgtcgttgct gcaggcctgc caggatcact
     4441 cctggccggg gaacctgcgc gagttgaaca attttctgaa gcgctacctg attctcggcg
     4501 acgagagcct ggcgctcacc gaactgcagc cgaaagacga cggcgggggc gggatgcgaa
     4561 gcaacagcgc agcccggggc tcggagccgg gaggcttgaa gtctctggcg cgctccgcca
     4621 aagacgaagc cgaggcgcag gccatcacgc aggccctgga acaaacgaac tggaaccgca
     4681 aacaggccgc cgcgattctg cagatcagct acaaggcgct gctctacaag atccggcagt
     4741 acgggatcgc cgaggccaag agccaccaca agctctccgc cggagcgtag agacgggggc
     4801 gagagctcag ggaccagcgc accttcaatc ctgcaatcgt gccattgtca tgttgagcgg
     4861 agggtgaaga tcgcgtcagc gatcgtcgcc ccgagtggaa gtatccctgc ctcccaccaa
     4921 ctttctccgg gactaacgaa tctcatggcc accgcgtgcg cctgacggcg cagtgcgcga
     4981 cgttcctacg gcacgatcag cgtcacgctc gtgctcttgg tgtcgctgcc cgaggtggcg
     5041 gtgatggtga tcgtgtaggt accgggaggc gttcccgatg gcggtatctg ggtcgtttgg
     5101 tggctgcagg caggcagcag cagaagcagg aaagacaaag cgcagagcgc aatgatcccc
     5161 atcagacggc gccggcgacg gtcttgagtg cccagaccca g
//
LOCUS       DASPUL010000038         5198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1513397, whole genome shotgun sequence.
ACCESSION   DASPUL010000038 DASPUL010000000
VERSION     DASPUL010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513397"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VEJ00_00830"
     CDS             <1..1263
                     /locus_tag="VEJ00_00830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HXW99729.1"
                     /translation="RARRSFELKAVVRLPPTQTTMAFSVVMPALEMAQETGKLIAWRK
                     KEGDRVTKGEPLLEIETDKAVVEVEAPADGVLAGVKAAEGADIPVGQTIAWIVAPGEP
                     PPTDGASSAAAPAARAVSQTRVETPRARASESSSAAPSARISPKARRLAKELGIDIAT
                     VRGSGPGGEILASDIQTAAAAPARSGAVVEKKSAQLEVPTSIGRIMAERTTQSWTTVP
                     HFFVSRDIDATALNEYRERHVSQSAAHHVRITHTDLLVALVARVLLKHPRLNASWSAE
                     GIRLHAYVNMGVAIAVNDGVVAAVIPNAHRATVAEIAAQRQAVAERARAGKLRPADIA
                     DATFTISNLGMYKVDAFSAIITPPQAAILAVGSIADRVVAINGKAVVRPMMTLTLSCD
                     HRVADGARAAMFLNDLAEAAREPANLLI"
     gene            1511..2485
                     /locus_tag="VEJ00_00835"
     CDS             1511..2485
                     /locus_tag="VEJ00_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99730.1"
                     /translation="MNSGTGNSGNFAGTAVAASGAGIASVTLNPATVTGGASTQITVH
                     LTAAAPAGGATVDLSNSDNAAVITPASVKVAAGLTTATTSASTKTVSAVTAVAITALY
                     NDTVAGAALTVDPAAATPSFTVADQPSSLTIAQGKSGSTKVTTKITTGYDHALKLTAS
                     GIPSGVSLSFSPSTIAAPGAGSSTAEIKVASSTQPGTYSIHLTATGTTSHSATLSLKV
                     PAPNPGPGATFQGCWYQQNGDKYQGVLISVENPGTYPFNATLYHGATCDPNNFADEFG
                     FGTDLNFGGFDYIFWFSDFHDQTDTSAIWQVGSNKSACVNYTVAPDCP"
     gene            2660..>5198
                     /locus_tag="VEJ00_00840"
     CDS             2660..>5198
                     /locus_tag="VEJ00_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXW99731.1"
                     /translation="MALATGTKLGPYEIQSLLGAGGMGEVYRAHDSRLDRTVAVKVLP
                     ASFSADRERLQRFAQEARAAAALNHPNILSIFDIGEAQGSPYVVSELLEGETLREKLR
                     NGPLPIRRVIDYALQVAKGLAAAHDKGIVHRDLKPENLFLTNDGRVKILDFGLAKLTR
                     PEAQSGSGDAPTVQVATEAGLVMGTAGYMSPEQVRGKPADNRSDIFTFGAILYEMITG
                     KRAFHGETAADTMSAILKEETPELAETTRNVPPGLERIVRHCLEKNPIQRFQSAGDLA
                     FDLDALTEISSSTKSGAQAAVQQVRAESSRRTIVAVACVVTLVAGMLALGWWLGRGGS
                     AASPPEYQQITFRTGYIGNARFTPDGSVVYSASWDGGDNQLYLARTDENGARELGLKD
                     AELLSISKSGELAIRLNTAFYGGYARSGTLARVPLSGGTPREVLENVQDAEWTGNGDS
                     MAVVKLTPENRHWRLEYPTGHVLLDTINWISDPHLSPDGKLIAFADHENPGGDDEGSV
                     AVIDMAGHEKKLASGFVSIEGIQWSPDGNEVWFTATRTGSATNLRGVTLAGKERTITN
                     VPGGMWLQDMRGGAALMITHRARLGIRGISPGGKEEKELGWFGWSVARDISRDGKKVL
                     FEEEGEGGGPNYTVFLRDTDGSPPVRMGEGLSVAISPDAKWVITRPAKGGPLNVVPTG
                     AGEARQLTHDNVAYDGARYVPDGKQLVAVGIEPGHGARDYLIDLSNGNSKPLTPEGYV
                     GRLLSPDGRAVAVIGPDGTRGIWPLDGGGFHAIPGLDRKYGVAGWTPDGASLLVVLNQ
                     ANGKTIKVSRVNVTTGKIDFWREFGDSLPTGANAGGPLFSADGNGYAY"
BASE COUNT         1053 a         1619 c         1617 g          909 t
ORIGIN      
        1 cgagcgcgta ggagtttcga gctgaaggcc gtagtccgcc ttcccccgac acaaacgact
       61 atggccttca gcgtagtaat gcccgccctc gagatggcgc aggagaccgg caagctcatc
      121 gcctggcgaa agaaagaagg cgaccgcgtc accaaaggcg agccacttct cgaaatcgaa
      181 actgacaaag ctgtggtgga agtcgaggcc ccagccgacg gagtgctggc aggagtcaaa
      241 gcggcggaag gtgccgacat tccagtcgga cagacgatcg cgtggatcgt ggcgcccgga
      301 gagccgcctc cgacagatgg ggcatcgtca gcggccgcac cggcagccag agctgtttca
      361 cagacaagag tcgagacacc gcgcgcaaga gcttcggagt cgagctccgc ggctccatcc
      421 gcccggattt cacccaaggc ccgccgtctt gccaaagagc tcggaatcga catcgcgacc
      481 gtgcgcggtt cgggtcccgg cggcgaaatc ctggcctcgg acattcagac cgcggccgcc
      541 gccccggcta gatccggcgc ggtggtggag aaaaagtcgg ctcaactcga agtgcccacc
      601 tccatcggcc gcatcatggc cgagcgaacc acacaaagct ggacgaccgt tccgcacttc
      661 ttcgtcagcc gcgacatcga cgccacggcg ctcaatgagt accgcgagcg ccacgtgtcg
      721 caaagcgcgg cccaccacgt gcgcattacg cataccgatc tgttggttgc gctggtggcg
      781 cgtgtgctgc tgaagcatcc gcgtctaaat gcgagttgga gcgccgaagg tatccgcctg
      841 cacgcttacg tcaacatggg cgtggccata gcggtgaatg acggggttgt ggcagctgtc
      901 atcccaaatg cccacagggc aactgtcgcg gagattgcgg cccagcgtca ggccgtcgcc
      961 gaacgcgccc gtgcgggcaa gttgcggcct gccgatatcg ccgatgcgac gttcacgatc
     1021 agcaacctgg gcatgtacaa ggttgacgcg ttcagcgcga tcatcacgcc gccgcaagct
     1081 gcgattctcg cggtcggcag tatcgccgat cgagtcgtcg cgatcaacgg aaaggccgtt
     1141 gttcgtccga tgatgactct caccctctcg tgcgatcatc gcgttgccga tggcgcacgc
     1201 gccgcgatgt tcctgaatga cctggccgaa gcagcccgag aacctgcgaa cttgctcatc
     1261 tagcgacagg taatcagcct taccacgtaa gaaagcacat gcggctgcct gcacttccga
     1321 ctgcgcggcg gtgtacttct gattcttgtg gcccgtcgtc tccgtgggaa aatcagagca
     1381 tcgggtctgc ttccgatttc cagcttcgag gtatttctcc gtgctcctcc tccaaaggaa
     1441 attcaaactc tcgcacctac tcccagccgg ggccttgctg gcgctcaccg cctgcggcgc
     1501 accgtcgaca atgaattccg ggacagggaa ctccgggaat ttcgccggca cagccgttgc
     1561 agccagcggc gccggaatag ccagcgtgac cctgaatccc gccacggtca caggcggcgc
     1621 ttccacgcag atcacggtcc atctgactgc ggccgcacct gccggcggag ccacggttga
     1681 cctgagcaac tctgataatg ctgctgtaat cacaccggca agcgtcaagg ttgcagcagg
     1741 actgaccact gctacgacct ccgcttcgac caagacagtt agcgcagtca ctgccgttgc
     1801 cattaccgcg ctctacaacg atacggtggc tggagccgcg ctcacggtcg atccggcggc
     1861 ggcgacgcct tcctttaccg tcgcagacca gccgtcatct ctgaccatcg cgcaaggcaa
     1921 atcgggctcg acaaaagtca ccacgaaaat cacgacggga tacgatcacg cgctcaagct
     1981 gaccgcttcc ggcattccct ccggcgtttc cctgagcttc agcccttcga cgatcgccgc
     2041 gcccggagcg ggctcttcca cagccgagat caaagtggca tccagcaccc agccgggaac
     2101 atattccatt cacctgacgg ccaccggcac gacctcgcac agcgccactt tgagcctgaa
     2161 agtccccgcc ccgaatcccg gccccggcgc gacatttcag ggatgctggt accagcagaa
     2221 cggtgacaag tatcaggggg tactcatctc ggtcgaaaat cctggaacct atccgttcaa
     2281 cgctactctc tatcacggtg cgacgtgcga tcccaacaac ttcgccgacg aatttggctt
     2341 tggcacagac ctgaactttg gcggattcga ctacatcttc tggttcagcg acttccacga
     2401 tcaaaccgac acgtcggcga tctggcaggt gggcagcaat aagtcggctt gcgtgaacta
     2461 caccgtcgca ccggactgcc cgtagcgaac gcgtccggtc cgtgatcctc cgggcgagct
     2521 ctgcggcagt tctttaacta cccgcgagca agagatattt caacgcagag gccgctgaag
     2581 ccgcgctgag aacgcagtga acagcgaatc agttcacctc cacgaaagtt tgcggctgca
     2641 ggtataatcg ccgcgacaga tggcgctagc caccgggacg aaactcgggc cgtacgaaat
     2701 ccaatccctg ctgggtgcgg gcggcatggg tgaggtgtat cgtgcccacg actcccggct
     2761 cgaccgcacc gttgcggtca aagtacttcc ggcctctttc tctgccgatc gcgaacgcct
     2821 gcagcgcttc gcccaggaag cccgggctgc ggcggcgctg aatcatccca acattctttc
     2881 gatcttcgac atcggagagg cgcagggctc gccctatgtg gtttcggaat tgctcgaggg
     2941 cgaaacgctg cgcgagaagt tgaggaacgg acccttaccc atccgccgcg tgattgacta
     3001 cgctttgcag gtggcgaaag gcctggcggc ggctcatgac aagggcatcg tgcaccgcga
     3061 tttaaagcct gagaatttgt ttcttactaa tgacggtcgc gtgaaaatcc tggatttcgg
     3121 cctggccaaa ttgacgcggc cggaggcgca aagcggcagc ggcgatgcgc ctacggtgca
     3181 ggtcgcaacc gaggcgggcc tggtgatggg aacggccggc tacatgtcgc cggagcaagt
     3241 gcggggaaag ccagccgaca atcgctctga cattttcacc ttcggagcga ttctgtacga
     3301 aatgatcacg ggcaagcgcg ccttccacgg cgagaccgca gctgacacga tgagcgccat
     3361 cctgaaggag gagacaccgg agcttgccga aaccacgcgc aacgttcccc cgggtctcga
     3421 gcggattgtg cggcactgcc tggagaaaaa tccgatccag cgctttcagt cggctggcga
     3481 cctggcgttc gatctcgacg cgctgaccga aatctccagc agcacgaaat ctggcgcgca
     3541 ggcggcggtg cagcaagtgc gtgccgaatc ttcgcgccgc acgatcgtgg ccgtagcttg
     3601 cgtggtgaca ctggtggcgg gcatgctggc cttgggctgg tggcttggcc ggggcggtag
     3661 tgcggcgtcg ccccccgaat accagcaaat cacgtttcgc accggctaca tcgggaatgc
     3721 ccgcttcacg cccgacggca gcgtcgtgta cagcgcgtcc tgggatggcg gggacaatca
     3781 actttatctc gcgcgaaccg atgagaacgg cgcgcgtgag ctggggttga aagatgccga
     3841 gttgctgtca atttcgaaga gcggtgagct ggcgatccgg ctcaacacgg cgttctacgg
     3901 cggctacgcg cgatctggga cgctggcccg cgtcccgttg agcggcggaa caccgcgcga
     3961 ggtgctcgaa aacgtgcagg acgcggagtg gacgggcaac ggcgacagca tggccgtcgt
     4021 caaactcacg cccgaaaacc gccattggcg gttggagtat ccgaccggcc acgtgttgct
     4081 cgacaccatc aactggatca gcgacccgca cctttctccg gacggaaagc tgatcgcatt
     4141 tgccgaccac gaaaacccgg gaggcgacga cgagggatcg gtcgccgtga tcgacatggc
     4201 ggggcacgag aagaaacttg cctccggatt tgtttccatc gaaggcatcc agtggtcgcc
     4261 cgacggcaac gaagtctggt tcaccgcgac ccgcaccggc agtgcgacca acctgcgcgg
     4321 ggtcacactt gcgggcaagg aacgcaccat cactaacgtg ccgggaggga tgtggctgca
     4381 ggatatgcgt ggcggcgcgg cgctgatgat tactcaccgg gcccgcctgg gcattcgcgg
     4441 catatcccca ggcggaaaag aagaaaagga actgggatgg ttcgggtggt ccgtggcgag
     4501 agatatcagc cgggacggca agaaagttct gttcgaggag gaaggcgagg gtggcggacc
     4561 gaattacacc gtcttcttgc gcgacaccga cggctcgcct ccggtgcgaa tgggagaagg
     4621 gttgtccgta gccatttcgc cggacgcgaa gtgggtgatc accaggccgg ccaaaggcgg
     4681 cccactgaac gtggttccga ccggcgcggg cgaggcgcga cagttaacgc atgacaacgt
     4741 cgcttacgat ggagcccgct atgtccccga cggtaaacag ttggttgcgg ttggcatcga
     4801 gcctgggcac ggagcacgcg actatctgat cgatttgagc aatggcaact ccaagccgct
     4861 caccccggag ggttacgtcg ggaggttgct ttcccctgat ggccgcgcgg tcgccgtgat
     4921 tggcccggac ggcacacgcg ggatctggcc gctcgacggc ggcggattcc atgccatccc
     4981 cgggctggac cgcaagtatg gcgtggcggg ctggacgccg gacggggcgt ccctgctggt
     5041 agttttgaac caggcaaacg ggaagaccat caaggtctct cgcgtgaatg tcacgaccgg
     5101 caagatagat ttctggaggg aattcggcga tagtcttccg acgggagcga atgccggcgg
     5161 tccactcttt tccgccgacg gcaacggcta cgcatacg
//
LOCUS       DASPUL010000039         7345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_280081, whole genome shotgun sequence.
ACCESSION   DASPUL010000039 DASPUL010000000
VERSION     DASPUL010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7345)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7345
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280081"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VEJ00_00845"
     CDS             complement(<1..434)
                     /locus_tag="VEJ00_00845"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXW99732.1"
                     /translation="MQLGMVGLGRMGANMTRRLMRGGHQLVVSDLSADAVKQLTSDGA
                     TGSVSLDDLVSKLTPPRAVWIMVPSGGPTEQTVQKLASRMQSGDAIIDGGNSYFKDDV
                     RRSRDLKGKGIHYIDVGTSGGVWGLERGYCMMIGGPKEAVQRL"
     gene            complement(616..2349)
                     /locus_tag="VEJ00_00850"
     CDS             complement(616..2349)
                     /locus_tag="VEJ00_00850"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HXW99733.1"
                     /translation="MNAPTIVSPLKTTLPEPLASSVGATAADWQSGGKMQRLWQRDAS
                     LWTGSDEANWLGWLDIVDEQIAQQDQLQKLAKEVQARGFQHVLLLGMGGSSLCPEVLR
                     MTFGRITHFATLHVLDSTDPAQVKAFEHQIDIAKTLFIVSSKSGSTLEPNIFKQYFFE
                     RTRQAVGATKAGSHFIAITDPKSHMQEVAESDRFLHVFFGRSSIGGRYSALSNFGMVP
                     AAVMGLDTKKFLSRAADMVRSCGASVPVEDNPAATLGVILGTAAKIGRDKVTIITSPG
                     ISDLGAWLEQLLAESTGKIGKGIIPVDREHLGASEVYGNDRVFAYLRLESGADAEQDA
                     KVAALEKAGHPVVRIAINDIYDLGAEFFRWEIATAVAGSIIGINAFNQPDVEASKVAT
                     RSLTAEYEKTGSLPAEKPVVEDSGIKLFTNEKNAAELSKAAGAPSLAGYLKAHLARIH
                     PGDYFAVLGYIQMNAEHEQALQAIRHAVRDKKRVATCLGFGPRFLHSTGQAYKGGPNS
                     GVFLQVTCDDSVELPVPGQKYTFGVVKAAQARGDFQVLAERGRRALRVHLGSNVKAGL
                     ATLRAAIEKAL"
     gene            complement(2353..3519)
                     /gene="tal"
                     /locus_tag="VEJ00_00855"
     CDS             complement(2353..3519)
                     /gene="tal"
                     /locus_tag="VEJ00_00855"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HXW99734.1"
                     /translation="MQPTGVLETSKATKNPLRELLNYGQSMWLDYIRRDLFTTGKLKQ
                     MIEEDGLRGMTSNPSIFEKAIAESSLYEDILKSLASRPDLDTTARFEQIAIRDIQDAA
                     DALRAVYEDSNFRDGYVSLEVSPYLARKTKETIDEARRLWKTVNRPNVMIKVPGTAEG
                     LPAIRQLIGEGLNINITLLFAQEVYEEVAEAYLAGLEDFARSGGSLKKIASVASFFIS
                     RIDTLVDSMLTDKLKSVSNATEEAFLKSLMGKVAIANGKLTYQRYQRIFSGPRWQALA
                     AKGAQTQRVLWASTSTKNPAYRDVIYVEELIGPDTVNTMPPATIDAFRDHGRLRNSLT
                     EDVAGAQKVMDDLTRAGISIRQVTDKLTDDGVKLFADAFDKLLAAVAKSTQKKG"
     gene            complement(3529..5610)
                     /gene="tkt"
                     /locus_tag="VEJ00_00860"
     CDS             complement(3529..5610)
                     /gene="tkt"
                     /locus_tag="VEJ00_00860"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXW99735.1"
                     /translation="MVASEQVLVTGKQLDTLCINTIRTLAIDAVQQANSGHPGAPMGL
                     APVAYCLWQQFLRYDPADPAWMNRDRFVLSNGHASMLLYAMLHLAEVRQLDAQGQATR
                     ELAVPMEEIKRFRQLGSRTPGHPESHLTSGIETTTGPLGQGVGNSLGMAIASKWYAAN
                     FNRPGFEIFDYNIYAMCSDGDLMEGIGGEAASLAGHLQLSNLCWIYDHNSVTLDGPAA
                     WSFSEDVATRFVGYGWNVTRVVDANDLEMLARAFEVFHKTTDRPTLIIVDSHIGYGSP
                     HKQDTNAAHGEPLGEEEVRLVKKFYGWPEDAKFLVPDGVRENFREGIGKRGRDLHARW
                     SELFAEYSQKFPDLAGRLHSMQHRELPAGWDKSLPTFPADAKGIATRESSGKVLNALA
                     QNIPWLMGGSADLATSNKTTLKFEGAGDLQAGSYAGRNLHFGVREHVMGASVNGMIVS
                     GIRAFGATFFNFSDYMRASIRLSALMEIPSIFIFTHDSIGVGEDGPTHQPIEQLASLR
                     AMPNLIVLRPGDANEVVEAWKIIAQLKHSPVALVLTRQAMPTLDRSKYSPTSGVARGA
                     YVLADAPGGKPDVILMGTGSELSLCVDAYEKLKAEGVNARVVSMPSWEIFEQQDATYK
                     ESVLPSSVTARVSAEMAATLGWERYVGLKGRMVGMHRFGASAPLKDLLKFFGFTVDRV
                     VAEARAAMGKS"
     gene            complement(5774..6418)
                     /locus_tag="VEJ00_00865"
     CDS             complement(5774..6418)
                     /locus_tag="VEJ00_00865"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXW99736.1"
                     /translation="MSTATGTDWTHILSDPDLVRHVGKLLQTYREASPEKREAALLAA
                     MREIKAGVSAAGKSSVPSAAAPAPQPMPTPASATPPFEPDLFAPNWSTDRRRHPRIKC
                     FVAVELRVDDIPTPIWGNLSNTSVGGCLVETATPVQPGANVDIGLWLPNGKIWVKGFA
                     LSGVVMRSGPARGVRVRFDALNPSERDSLRQFLKFVQETTKTSQGENGYLQLLK"
     gene            complement(6531..6896)
                     /locus_tag="VEJ00_00870"
     CDS             complement(6531..6896)
                     /locus_tag="VEJ00_00870"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXW99737.1"
                     /translation="MSSQSARTAFTSTERRKRRHPRYRSEFPVTLTLLAEEEHRRLDG
                     HCRDLSVAGIGILVASELNLGEVVSLAFSLQAQPWNVRAVLRYRRGYHYGLEFLSLSD
                     QQSAELMAHLSGLERADSD"
     gene            complement(6945..7277)
                     /locus_tag="VEJ00_00875"
     CDS             complement(6945..7277)
                     /locus_tag="VEJ00_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXW99738.1"
                     /translation="MRIRVALLALAFMPLSSFMAAQNTSATARAGRRVMIRTAPTYPE
                     LAKRMHVQGTVKIEATVRPNGTVKSTRVIGGSPVLVGAAIDAVSRWKFEAAQTESLEI
                     VQVNFDDQ"
BASE COUNT         1420 a         2145 c         2246 g         1534 t
ORIGIN      
        1 agacgctgca ctgcctcttt cgggccgccg atcatcatgc agtaaccgcg ctcgagtccc
       61 cagacgccgc cgctggttcc gacgtcaata tagtgaatgc ccttgccctt caaatctctg
      121 gagcggcgga cgtcatcctt gaaataggaa ttgccaccgt cgatgatggc gtcgccggac
      181 tgcatgcggg acgcaagctt ctggacagtc tgttccgtcg gaccgccgga agggaccatg
      241 atccagaccg cgcgcggggg cgtgagcttg gagaccagat cgtcgagcga tacagagccg
      301 gtcgcgccgt cgctggtcag ttgcttgacg gcgtcagcgc tgagatcgga tacaacgagt
      361 tgatgtccgc cgcgcatgag ccggcgcgtc atgttcgcgc ccatgcggcc cagacctacc
      421 attcccaatt gcatgtgtgc tcctcgtgcg cttacccggg ggctaaagcc ccgcaccggt
      481 ggggccgctg gacggcgcgg ctacaagccg cgccctttca aagcctcttc agggaaatcg
      541 ctttgaaaca aatctcttta tgacaaatct ctccataaca agtcttctcg acgcaggtct
      601 cttaacagag acctctcaca gtgccttctc gatggctgcc cgcagtgtgg ccaggccggc
      661 cttcacgttg cttccaagat gcacgcgcaa ggctcttctt ccacgctccg ccagcacctg
      721 gaagtcgccg cgcgcctgcg ctgccttcac gacgccaaag gtgtatttct gccccggcac
      781 cggcaactcg accgaatcat cgcaagtcac ctgcagaaaa actccggagt tggggccgcc
      841 cttgtaggct tgcccggtcg aatgcaggaa acgggggccg aatcccagac aggtggccac
      901 acgcttcttg tcgcgcaccg cgtggcgaat cgcctgcagg gcctgttcgt gctcggcgtt
      961 catctggatg tagccgagca cggcgaaata atcgccggga tggatgcgcg ccagatgggc
     1021 tttgaggtat cccgccagcg atggagcgcc ggcggccttc gacaactctg ccgcgttttt
     1081 ttcatttgtg aaaagcttga taccggaatc ttcgacgacc ggtttttccg cgggcaagga
     1141 accggtcttt tcgtattcgg cggtcaggct gcgggttgca accttgctgg cttcgacatc
     1201 gggctgattg aaggcgttga taccgatgat cgagccggcc acggcggtgg cgatttccca
     1261 gcggaagaat tcggcaccga ggtcgtagat atcgttgatg gcgatgcgca cgacgggatg
     1321 gccggccttt tcaagggccg ctacctttgc gtcctgctcg gcatcggctc cggattccag
     1381 gcgaaggtag gcaaacacgc ggtcgtttcc atagacctcg gaagcgccga ggtgctcgcg
     1441 gtcgaccggg atgattccct tgccgatttt cccagtggac tcggcaagca gctgttcgag
     1501 ccaagcgccc aggtcagaaa tgcccggaga agtgatgatc gtgactttat cgcgcccgat
     1561 cttggcggcg gttcccagaa ttacgccgag cgtggcagcc gggttgtctt cgacggggac
     1621 gctggcgccg caggagcgca ccatatccgc ggcgcgcgag aggaatttct tcgtatccag
     1681 gcccatgaca gcagcgggaa ccatgccgaa gttcgagagc gcggagtaac gaccaccgat
     1741 cgaggaacgc ccgaagaaaa cgtgcaggaa gcgatcggat tcggcaacct cctgcatgtg
     1801 ggacttggga tcggtgatgg cgatgaagtg gctgccggcc ttggtcgctc cgactgcctg
     1861 tcttgtgcgc tcgaaaaaat attgcttgaa gatattcggt tccagcgtgc tgccggattt
     1921 gctggagacg atgaagaggg ttttcgcaat gtcgatctga tgttcgaacg ccttgacctg
     1981 ggcaggatcg gtggagtcga ggacgtgcag cgtggcgaag tgcgtgatcc ggccgaaggt
     2041 catccgcagc acttcgggac agaggctgga gccgcccatg cccagaagca gcacgtgctg
     2101 aaagccgcgc gcctggactt ccttggcaag cttctgtagc tggtcttgtt gggcgatctg
     2161 ctcgtcaacg atgtcgagcc atccgagcca attggcttcg tcggaaccgg tccagaggga
     2221 ggcatcgcgc tgccacaaac gttgcatctt gccgcccgat tgccagtctg cggccgttgc
     2281 tccaaccgat gatgccaaag gttcgggcag ggtagtcttg agtgggctca cgatcgttgg
     2341 tgcgttcaca actcacccct ttttctgcgt gctcttggct actgcggcaa ggagtttgtc
     2401 gaaggcgtcg gcgaacagtt tgacgccatc atcggtcagc ttgtcggtga cttgcctgat
     2461 ggaaatgccg gcgcgtgtca gatcgtccat tactttctgc gcgcccgcga cgtcttcggt
     2521 gaggctgttg cgcagtcgtc catgatcgcg gaatgcgtcg atggtggccg gcggcatggt
     2581 gttcacggtg tcggggccga tcaactcttc cacgtagatg acgtcgcgat aggccggatt
     2641 cttggtgctg gtgctggccc agagcacacg ctgggtctga gcgcccttgg cggcgagagc
     2701 ctgccagcgt ggtccgctga aaatgcgctg gtagcgctga tatgtgagtt tgccgttggc
     2761 aatcgcgacc ttgcccatga ggctcttgag gaaagcttcc tcggtcgcat tggacacgct
     2821 cttcaacttg tcggtcagca tggagtcgac cagggtgtcg atccggctga tgaagaaact
     2881 ggcgacgctg gcaattttct tcaggctgcc gccgctgcgc gcgaaatctt caaggccggc
     2941 aaggtacgcc tcagcgactt cctcgtagac ctcctgcgcg aacagcagcg tgatattgat
     3001 gttgaggccc tcgccgatca actgccggat ggcgggaagt ccctcggccg tccccggcac
     3061 cttgatcatg acgttcgggc ggttcaccgt tttccatagg cggcgagcct cgtcgatggt
     3121 ctccttggtc ttgcgcgcga gataaggtga gacctcgagg ctgacgtagc cgtcgcggaa
     3181 attcgagtct tcgtacaccg cgcgcagggc gtcggccgca tcctggatgt cgcgaatcgc
     3241 gatctgctcg aagcgggcgg tcgtgtcgag atcgggacgc gaggccaacg actttaggat
     3301 gtcctcgtag agcgaacttt cggcgatcgc cttctcgaag atcgaaggat tcgacgtcat
     3361 gccgcgcaag ccgtcttctt caatcatctg tttcagcttg ccggtggtga acagatcgcg
     3421 ccggatgtag tcgagccaca tcgactggcc gtagttcagc aattctcgta gcggattttt
     3481 tgtcgctttc gaagtttcca gaaccccagt tggctgcatg atgacctcct atgatttccc
     3541 catggctgcg cgtgcttctg cgacgacacg gtccacagta aacccgaaaa acttcagaag
     3601 atccttcaaa ggggcggagg cgccaaagcg atgcatgccc accatgcgcc ccttaaggcc
     3661 gacataacgt tcccagccga gggtcgcggc catttctgcc gagactcgcg cggttacgct
     3721 ggagggcaga acgctttcct tgtaggtggc gtcctgctgc tcgaagatct cccacgacgg
     3781 catgctgacc acgcgggcgt tgacgccctc cgctttgagt ttctcgtaag cgtcgacgca
     3841 gagagagagt tcgctgccgg tccccatcag aatcacgtca ggctttcccc ctggtgcatc
     3901 ggcgagcacg taggcgcccc tggcaacacc ggaggtcggt gagtatttcg agcgatcgag
     3961 agtcggcatc gcctgccgtg tcagcaccag cgccaccgga gaatgcttca actgcgcgat
     4021 gatcttccag gcttcgacga cttcattggc gtctccaggg cgaagcacga tgagattcgg
     4081 catcgcccgc aatgacgcca actgctcgat cggctgatgc gtaggcccgt cttcgccgac
     4141 gccgatcgag tcatgcgtga agatgaagat ggaagggatc tccatcagag ccgatagccg
     4201 gatgctggcc cgcatgtagt cgctgaagtt gaagaaggtc gcaccgaagg cccgaatacc
     4261 ggaaacaatc atgccgttca cgctggcgcc catgacatgc tcgcgcacgc caaaatgcag
     4321 gttgcgtccg gcgtagctgc cggcttgaag gtcgccggca ccctcgaact tcagtgtggt
     4381 tttgttggaa gtcgcaagat cggcggaccc gcccattaac cagggaatgt tctgcgccag
     4441 agcgttcagc actttgcctg agctttcgcg ggtagcgatg cctttcgcgt cggcggggaa
     4501 ggtgggcaaa ctcttgtccc agccagcggg cagctcgcga tgctgcatcg agtgcagccg
     4561 tccggccaga tcggggaatt tttgcgagta ctcggcgaat agctccgacc agcgcgcatg
     4621 cagatcgcgc ccgcgctttc cgatgccttc gcgaaagttc tcgcgcaccc catcgggcac
     4681 caggaatttc gcgtcttccg gccagccgta gaacttcttc accagccgta cttcttcttc
     4741 acccagaggc tcgccatgag cggcgttggt gtcctgcttg tgcggcgatc cgtatccgat
     4801 gtggctgtct acaataatca gcgtcggccg gtcagtggtt ttgtggaaga cctcgaaggc
     4861 tcgcgccagc atttcaagat cgttagcatc gaccacgcgc gttacgttcc agccgtagcc
     4921 gacaaaccgc gttgcaacat cttcactgaa cgaccatgcg gcaggtccgt cgagcgtgac
     4981 ggaattgtga tcatagatcc agcagagatt cgaaagctgc agatgtccag ccagcgaagc
     5041 cgcttcacca ccaatacctt ccatcagatc gccatcgctg cacatcgcgt agatgttgta
     5101 gtcgaagatc tcgaaaccag gacggttgaa gttggccgca taccacttcg atgcgatcgc
     5161 catgcccagg ctgttgccaa cgccctgacc cagggggccg gtcgtggtct caattccaga
     5221 agtaagatgc gattcggggt ggcccggcgt acggcttccc agctggcgga accgtttaat
     5281 ttcctccatg gggacggcta gttccctggt ggcctggcct tgtgcgtcaa gctgccgtac
     5341 ctccgccagg tgcagcatgg cgtagagcag catggaagcg tgaccgttgg agagtacgaa
     5401 acggtcgcga ttcatccagg cgggatcggc gggatcgtag cgcaggaatt gctgccataa
     5461 acagtacgca accggcgcca atcccatggg agcgccggga tggcccgagt tggcctgttg
     5521 tacggcgtca atcgccagag tccgaatcgt attgatgcac agtgtgtcga gttgcttccc
     5581 tgtaactaaa acctgctcac tggctaccat gcggctctgc tcctccagcc gaaacttgac
     5641 tccggcgtgc gcccagaaag tctcaaccga ttagagttac gaggtacgga aagggttcaa
     5701 tagacagaaa gtgtatcaat ttccgggagt gtgtggattg acctgctgta aaaagcgaag
     5761 gcgggcgggg atgctacttc agcagttgca gatagccgtt ttcaccctgt gaagttttgg
     5821 tggtttcctg cacgaacttc aggaactggc gcaagctgtc gcgctcggac ggattcaagg
     5881 cgtcgaagcg aacgcgcacc ccgcgggcgg ggccgctccg cataaccact ccactgaggg
     5941 cgaagccttt cacccagatt tttccgttgg ggagccagag cccgatatcg acattagccc
     6001 ccggttgaac gggggttgcg gtttccacca ggcaacctcc cacgctggtg ttggagagat
     6061 tgccccagat gggagtcggg atatcgtcca cgcgcagttc taccgcgacg aagcacttga
     6121 tgcgcggatg acggcgccga tccgtgctcc agttgggagc gaaaagatcc ggctcaaaag
     6181 gaggcgtggc tgaagccggg gttggcatcg gttgcggggc cggggccgcg gccgatggga
     6241 cagacgattt tccagccgca gagacacccg ccttgatctc gcgcatggct gccagtagtg
     6301 cggcttcgcg tttttccggc gacgcctcgc ggtaagtctg gagcagtttt cccacgtgcc
     6361 gaaccagatc cggatcagac agtatgtgag tccagtctgt gcctgtcgca gtgctcattg
     6421 aatacgcctt cacctgcccg cgtggggcct cagttcaagg ttgaactggc gggaactcgg
     6481 agacaagatg accaaagtca taggcgttcc gtggggatgt cctccccgag ctagtcggag
     6541 tcggcccgct ccaggcccga gagatgcgcc atgagctctg ccgactgctg atcagaaaga
     6601 gaaagaaatt caaggccgta gtgatatccg cggcgataac gcagtacggc ccgcacgttc
     6661 caaggctgag cttgcagaga aaaggcaagc gacacgactt cacccaggtt aagttcggaa
     6721 gcaaccaaaa tcccgattcc ggcaaccgac aggtcccggc aatgcccgtc gaggcgccgg
     6781 tgttcttcct ccgccaagag agtcagcgtc accggaaatt ctgagcggta gcggggatgc
     6841 cgccgcttcc ggcgctcggt gctggtgaag gctgttcttg ctgattggga tgacatcgtt
     6901 cgtgttcgcg aggcttgttc aatcttgcag gcagaccgcg agctctactg gtcgtcgaag
     6961 ttgacctgga ctatttcgag gctctcggtt tgtgcagctt caaacttcca ccggctaacg
     7021 gcatcgatcg ccgcgcccac caggaccgga cttccaccaa taactcgagt agacttgacg
     7081 gtgccgttag gccgcactgt ggcttcgatt ttgaccgtac cctgaacatg catccgcttt
     7141 gcaagttcag gataggtggg ggccgtgcgg atcataaccc tgcgtcccgc ccttgccgtc
     7201 gcggaagtgt tctgtgccgc catgaaactg ctgagcggca taaaggcgag ggccagaagc
     7261 gcgacacgaa tccgcatgca agacggtcct tggtttccac attaccgggc gcgtttgccg
     7321 cgagcaaagt actgaagtca ctgca
//
LOCUS       DASPUL010000040        13933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_691275, whole genome shotgun sequence.
ACCESSION   DASPUL010000040 DASPUL010000000
VERSION     DASPUL010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 13933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13933)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13933
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691275"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VEJ00_00880"
     CDS             <1..1151
                     /locus_tag="VEJ00_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXW99739.1"
                     /translation="LSSSGGAAALLADHGGEMGVPLAEFSAGTAERINKLLPGFARKE
                     NPIDLTGQINSVANLFRDTCLTVAADPRTEAVVVQHANSGRRYLKEDGEVYKTVARDM
                     PVVVSFVGDLLPPETRREYLEAGVLLAPEPSAAMSGLSVLYKLRNYRPATVKPKPTGP
                     ARQVPQGWADTMALCSEAGATPANWVILRPSDRAEAACAALKFPVVVKVLPSEADHKT
                     ELGLVRLGVKTPAEVDGLATDFRARMGKPGAGILVQEMVEGGGVEVVLSFLRNTDFGP
                     VMSIGTGGVAIELYRDITHLALPVDADQVRAVLPKLKLWTLLQGFRGKPPADIDALVD
                     AAVRLGDIFLATPGLQELELNPVIVTRKGTGLRVVDALVVGSRADKPK"
     gene            complement(1157..2704)
                     /locus_tag="VEJ00_00885"
     CDS             complement(1157..2704)
                     /locus_tag="VEJ00_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXW99740.1"
                     /translation="MSLIAASALTTPRDRNASALSGDSNTASPGFLSNPDKQHWIDGK
                     WVASLAGERIETFNPTTGRLLATLARGREADVDAAVKSARRAFQGPWSSFTAGQRQRL
                     LMRFAELFEKHFDELTLLESLDMGAPLITRLRPAKNGALQTILYFAAQARNLGGELPG
                     HSLPGAFTTMLVRAPVGVVGGIVPWNGPLFGQMHILGPALATGCTVVLKPAEDASLAI
                     LRTAELLVEAGVPPGVVNVVTGLGSEAGAALASHMDVDRVAFTGSTATGRAIIKASAG
                     NMKRLQLELGGKSPDIVFADADLDKAVPGVAMGVFSNSGQICFAGTRVFVQRSIQDEF
                     VERLGAYSKTLKVGDPLAADTNLGPLISRRQLDRVLQYVGIGSQEGAQLVAGGKRLTG
                     ELSDGYFIEPTLFSNVSNSMTIAREEIFGPVASVIGFNTADEALALGNATDYGLASGV
                     WTRNINTALKMANGIQAGTVWVNCYGVVDPSVGFGGYKQSGYGWKGGRAQIDGYLYQK
                     AVYIDGN"
     gene            complement(2716..3786)
                     /locus_tag="VEJ00_00890"
     CDS             complement(2716..3786)
                     /locus_tag="VEJ00_00890"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXW99741.1"
                     /translation="MRGVVFTGHRNLELVAFEDPVPGDDEVIIEMKASGFCGSDLHHY
                     RGVRGASLRAKSPEFLAERKLNVEDPIIAGHEPCGIVAEVGKNVDQRSFRKGDRVMVY
                     HYEGCQYCDPCRTGWVQMCDRGATVFGQTAHGGHAPYMKAPARSLIHLPSDISFSAGA
                     AISCGTGTAFAALERVQLSGRDTLAVFGLGPVGQSAVQLASAMAVRTFAVDVSDERVS
                     QAKTFGAVDGVNSKTADPVEAILQWTHGVGATVAIDCAGVAAARQAAVRCTSNWGRIA
                     FVGVGGDVTLDAWSDLMVKQRTVIGHWTFSDTGMARCVRFVADHGIDVDKQFSDRWKL
                     DDADVAYQKFDNQTAGKAVFEF"
     gene            4115..4888
                     /locus_tag="VEJ00_00895"
     CDS             4115..4888
                     /locus_tag="VEJ00_00895"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXW99742.1"
                     /translation="MGKLDGKVALVTGAARGIGREISLTLAREGADIVANALNAPNLE
                     TLKAEVEKLGREAITAIADVSKKAAVEAMVDAAIREFGKIDILVCNAGITRFAPFLEM
                     TEEDWDIVVDVDLKGTFLCGQAVAKHMVKRKYGKIVNISSASGVGARNHTMANYAASK
                     AGAINLSRVMALALGDYGINVNVVAPGVIKTEMGLSRRTPEEWAAYLELYKQQTALHR
                     VGEAKDIANAVLFLVSDDSSFITGQVILSDGGLKDGLLR"
     gene            5075..6055
                     /locus_tag="VEJ00_00900"
     CDS             5075..6055
                     /locus_tag="VEJ00_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXW99743.1"
                     /translation="MNPIRRWLFAIFIASLIFPAVTSAQTWPDKPVHMTMPFPPGTGP
                     DIVMRTVSERLAKMWKQPVLVENKPGGNGFIAMSAAKRAPADGYTLVMVDYGIMTVLP
                     YLFPKTIPYDPVKDFDAVAPMYWAYWFVAVAGDSRFTKIPDLVAEANAKNGTMTYGSS
                     GVGSPMHLQAAMLENATGTHMSHIPYKETPQVLIDISRHDIGWAFTTGATGGGMAKGG
                     KIKFLAVASPKRHPGFPDVPTMAEAGGPKDFDLRTWVGLYAPHGVPKPILDKISADVA
                     NVLSDPEVKDLLVANGLEAWPGPASELVKQQAEDSKKYGELTSKLKISLD"
     gene            6137..6976
                     /locus_tag="VEJ00_00905"
     CDS             6137..6976
                     /locus_tag="VEJ00_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXW99744.1"
                     /translation="MDHLNPSGPGWTPPRRVDGKVAVVFGAGQSPGEGLGNGRAAAIR
                     YAREGAKVLAANRSIESAEATAELIRAEGFDCVAVVADVRRESDIASAIDEAQRRWGT
                     VDILHNNVGVSLGGGDTDVLNITEEAFDNIYQSNLRGTAFACKYALPIMRKQRSGAIV
                     NISSAAAVGLYPYVAYKATKAGVVALTEQLALQNAPYGIRVNCILPGLIDTPMAVETR
                     SKKWNQPRDQVLAERVAKIPLGRQGTGWDVANLSLFLASDEAAFITGVSILIDGGRVL
                     NRI"
     gene            7000..8700
                     /locus_tag="VEJ00_00910"
     CDS             7000..8700
                     /locus_tag="VEJ00_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXW99745.1"
                     /translation="MGPQHDTYDVIVVGAGAAGLCAASTAAALGKSALVLEHTAKVGG
                     TTAISGGMVWIPVNRKMAEAGIPDSLDAAWRYLGSTVPGCEQDPLMSAFIARGNEAID
                     FLDSRTSVKLRPVRRYPDYYPDLPGATAGGRVLEPVPFDGRALGKDFSLLRDPIPEFL
                     LFGGMMVSREDVPVFRRVGSSVRALWHVSKLVGRYAGQRLRAHRGTSLVLGNALAARL
                     LKSARDLGVEIIVGASVRQLEASENRVTGVSLEIAGRATKLQARCGVILATGGISHYP
                     ELRRAYVPASAGNLSATVSSGAASGGVQLAKELGADVSPPSANLERALAWWVPVSRFQ
                     RSNGATAVFPHVVTDRAKPGLIAVNKEARRFVNEAVSYHEFVRAQLRDPERSIPAWLV
                     CDSRFMWKYGLGRVRPFTLSLRAEVGSGYLKRARSLDELAKSIGLPVAEFRNTVQRYN
                     ESARIGKDPEYGRGSNIYQRHLGDADHHPNPCVAPIEKPPFYAVEVFPADLGMAAGIS
                     TDEYGRVLRANEPVHGLYACGNDMHSVMNGAYPGPGITLGPALVFGYVAAFHACAERT
                     "
     gene            complement(8633..9706)
                     /locus_tag="VEJ00_00915"
     CDS             complement(8633..9706)
                     /locus_tag="VEJ00_00915"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXW99746.1"
                     /translation="MDLRQLRYFVQIVESGSLTKASRRLFIVQPALSQQMARLEEEVG
                     RPLLVRSSRGVAPTSNGRALYHHAKFMLRQLDEAVVIARQDRSDIKGRVTLGLAPTTA
                     AALGMPLIRQLRARHPGIDLSIVSLHSGYLEEMARAGQLDVAIMFSHSAASEMTLEPL
                     LEEELFVLVSADSDLVPRDKTSLSLNEIARMPLALPRAGHGLRRRVDLELDRAQLTVR
                     EPVAEIDSLLLLMQYVAEGAGVTIQPMSAMLVLEFPGRWRCLSISQTRMLRTNYLYAL
                     PPDKISTTASTVRAELKNITNDLVSSGIWRGVRLVNTESLDTGTDSIPKSIAEADTSG
                     LIRFARRTREMPQHNRRRERAQV"
     gene            complement(9754..11262)
                     /locus_tag="VEJ00_00920"
     CDS             complement(9754..11262)
                     /locus_tag="VEJ00_00920"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HXW99747.1"
                     /translation="MDILHNLAFGFEHALTLQNLLFCAIGCVVGTLVGLLPGLGPLAT
                     ISLLLPLTYSIPVTGALIMLAGIYYGAQYGDSVSAITMKIPHASSIVACIDGYQMTLK
                     GRTGLALFTAGVSSFIGGTVAIVVLAALAPALGEVALMFGPADYTALMLFGFFCVSFV
                     TTGSLLNGLAMCFIGVLLGQIGTDVNSGVLRYTFGLPFLADGVGVVSVALGCFGIAEI
                     AKNLDGHEDRSPFKGKIKLMPTWPEFKRIIPSALRGSVVGSALGILPGGGPVIAQFAA
                     YALDKKVSKYKDEIGKGSIEGVAGQAAADEAAARTSFIPLMSIGIPENAVMALMMAAF
                     IIKGIQPGPNMISGHPELFWGLVASMWIGNVFLLVLNVPLVRYWLSVFKIPYQVLFPA
                     ILFFCCIGTYSINNNLEDVYITAAFGLAGYLFLRLELDPAPLMLGFILGPMMEENFRR
                     SLLLSRGSFSTFVTRPISGTLLALIALLIAWQLLVFFKRTTKDVKVPVAVEQ"
     gene            complement(11262..11681)
                     /locus_tag="VEJ00_00925"
     CDS             complement(11262..11681)
                     /locus_tag="VEJ00_00925"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HXW99748.1"
                     /translation="MNQNLARGVALTAIALGFGLGAFRYPIGQFERAGPGLFPLVVSS
                     FLLLIGITTVIRSLLIERQPMQFQFKNIAIILLSLCGFALLSEFVNMILGIVFLVFFA
                     MLADPPYSIKRNLVVAAVLVAIALGFQQLLGLQLPLF"
     gene            complement(11766..11903)
                     /locus_tag="VEJ00_00930"
     CDS             complement(11766..11903)
                     /locus_tag="VEJ00_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99749.1"
                     /translation="MQITAMLYGSRLLNFVGFPSSEVLGPDASEEAIKALLDRHGELF
                     V"
     gene            complement(11914..13125)
                     /gene="tcuB"
                     /locus_tag="VEJ00_00935"
     CDS             complement(11914..13125)
                     /gene="tcuB"
                     /locus_tag="VEJ00_00935"
                     /inference="COORDINATES: protein motif:HMM:NF011607.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HXW99750.1"
                     /translation="MPPLESQPPVQSLDGLTRQAAALARGSARVIPIASQSLNGDETE
                     AARVLQICNACRYCEGFCAVFPAMTRRLTFGKGDVNFLANLCHNCGACLHACQYAPPH
                     EFAVNVPQAMAKVRLGTYTEYAWPPALGALYRRNGLTIALALAGALAAFLILAVAIHG
                     PLWQSVPAGNFYAVFPHNLMVAMFGPVFLFAALALAIGAWRFWQDERPGEPTQGAVGE
                     AIGNILTLKYLDGGHGDGCNDENDRFTERRRLFHHFTFYGFLLCFAATSVATLYHYVL
                     GWSAPYAYTALPKLLGLSGGVALLIGTAGLFWLNLRRHPLQQDSAQRPMDRGFIALLF
                     LTALSGIVLMLVKATPALPLALSVHLGTVMALFLTLPYGKFAHGVYRAAALLKWSVER
                     RKPSDLQLSEE"
     gene            complement(13112..>13933)
                     /gene="tcuA"
                     /locus_tag="VEJ00_00940"
     CDS             complement(13112..>13933)
                     /gene="tcuA"
                     /locus_tag="VEJ00_00940"
                     /inference="COORDINATES: protein motif:HMM:NF006130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HXW99751.1"
                     /translation="GVNERGEYPADNFLIRGTRFNTGELLRFMIDAGADTIGDPTQAH
                     MVAIDARAPLYDGGICTRIDCVSLGVVVNREAKRFYDEGEDFWPKRYAIWGRLVAQQP
                     GQVAYSIIDAKAVGRFMPPVFPGTRTGTLPELARALGLDEGRFVQTLNEYNAACRPGT
                     FDHTALDDCRTAGLAIQKTHWARPIDTPPFYGYAVKPGVTFTYLGLRVDETAAVRFSG
                     RASTNLFVAGEMMAGNVLGKGYTAGVGMSIGTAFGRIAGTQAANSALKETSRAAA"
BASE COUNT         2892 a         4138 c         4200 g         2703 t
ORIGIN      
        1 ttctcagttc gtcgggcggc gccgcagcgt tgctggcgga ccacggtggc gagatgggtg
       61 tcccgctggc cgaattcagc gccggcaccg ccgagcgcat taacaagctt ctgccaggat
      121 ttgctcgaaa ggaaaatccg atcgatctaa cggggcagat caacagcgtg gctaatttgt
      181 tcagggatac gtgcctcacg gtagcggcgg atccgcgaac tgaggcggtg gtcgtgcagc
      241 acgcaaacag cgggcgacgg tacctcaagg aagacggcga agtctacaaa accgtggcgc
      301 gcgacatgcc tgtggtggtg agctttgtcg gggacctctt gcccccggaa acccgacggg
      361 aatacctgga agcaggagtc cttctcgcgc cggaaccttc ggcggcgatg agcggcctgt
      421 cggtgctgta caagcttcga aattatcgtc cagcgacggt caagccaaaa cccaccggtc
      481 ccgctcgcca ggtgccgcag ggctgggccg atacgatggc gctttgcagc gaagcaggcg
      541 ccacgccggc gaactgggtc atcttgaggc cgtcggatcg cgcggaggct gcctgcgctg
      601 cattgaagtt ccctgtcgtg gtcaaggtgc ttccttcgga agccgaccat aaaaccgaac
      661 tcggcctcgt ccggctgggt gtcaagacac ctgccgaggt cgacggctta gctaccgact
      721 ttcgtgcacg gatgggcaaa ccgggcgcag gcattctcgt gcaggagatg gttgaagggg
      781 gaggcgtcga ggtagtcctg tcattcctga ggaacaccga ctttggtccc gtcatgtcga
      841 tcggaaccgg tggggtggcg atcgagcttt atcgcgacat tacgcatctt gcgctcccgg
      901 tagacgctga tcaggtccgc gcggtactcc cgaagctcaa gctttggacg ctattgcagg
      961 gattcagagg caagccaccc gccgatatcg atgccttggt ggacgccgcg gtacgccttg
     1021 gcgatatctt cctggcgacg cctgggcttc aggaattgga gctcaatccc gtcatcgtga
     1081 cccgcaaggg gacgggcctg cgagtggtcg atgcgcttgt cgtaggctca cgagccgaca
     1141 agcctaagta agcggactag ttgccgtcga tgtaaacggc cttctggtac aggtagccgt
     1201 cgatttgcgc gcggccgccc ttccagccgt agccgctttg cttgtaaccg ccgaatccca
     1261 ccgaaggatc caccacgccg taacagttca cccagaccgt acctgcctgg atgccattgg
     1321 ccatcttgag cgctgtattg atattccgcg tccacacccc gctggccagt ccatagtcgg
     1381 tggcattgcc cagtgccaac gcttcgtctg ccgtattaaa tccaatcacc gaagcgaccg
     1441 gcccgaagat ttcttcccga gcgatggtca tcgaattgga tacattgctg aagagtgttg
     1501 gctcaatgaa gtagccgtcg ctcagctctc cagtcagacg cttgccgccc gcgaccagct
     1561 gggcgccttc ctgcgagcca ataccgacgt actgaaggac gcggtcgagc tgacgcctcg
     1621 atatcagagg gcccaggttg gtgtcagccg caagggggtc accaaccttc agcgtcttgc
     1681 tgtaggcacc cagccgctcg acgaattcat cttgtatcga ccgctgaacg aatactcggg
     1741 tacctgcgaa gcagatctgc ccgctattgc tgaatactcc catggcgaca cctgggaccg
     1801 ctttgtcgag gtctgcatcc gcaaacacga tatccggcga cttaccgccg agctcgagct
     1861 gtagacgctt catattgcct gccgatgcct tgatgatcgc gcggcccgtg gccgtggagc
     1921 cggtaaaggc gacgcgatca acatccatat ggctcgcaag cgctgcgcca gcctcgctgc
     1981 ccagacccgt cacaacgttg acgaccccgg gcgggacgcc ggcctcgacc agcagttccg
     2041 ccgtgcgcag tatcgcgagc gaagcgtcct cggccggctt cagtaccacg gtgcagcccg
     2101 tggccagcgc cggaccgaga atgtgcatct gcccaaatag cgggccattc caagggacaa
     2161 tgccgccaac gacgccgacg ggtgccctga cgagcatggt tgtgaaagcc cccggaagcg
     2221 agtgccccgg cagttcgcca cccaagtttc gcgcctgggc cgcgaagtac agaatcgtct
     2281 gtagggcacc gttcttggcc gggcgcagac gcgtgatcag tggcgcaccc atgtcgagcg
     2341 attcgagcag cgtcaactcg tcgaaatgct tctcgaaaag ctcggcaaag cgcatgagca
     2401 ggcgttggcg ctgtccggcc gtaaaactgc tccatgggcc ctgaaaggca cgccgggctg
     2461 actttacggc agcatcgaca tctgcctctc tgccgcgtgc cagcgtcgcg agcaacctac
     2521 ccgtggtcgg gttgaaggtc tcaattcgct cgcccgccag ggaagcgacc catttgccat
     2581 cgatccagtg ctgcttgtca gggttcgaca ggaatccagg gctggcggta ttcgaatcgc
     2641 cactcaatgc acttgcgttg cggtcgcgcg gcgtcgtgag ggctgaagcg gctatgaggg
     2701 acacggttga gcctttcaaa actcaaatac cgctttgccg gcggtctggt tgtcgaattt
     2761 ctggtacgcg acgtcggcat catcaagctt ccagcggtcg ctgaactgct tgtcgacgtc
     2821 aatgccgtgg tccgcaacga aacgcacaca cctggccatt ccggtatcag agaaggtcca
     2881 atggccgatc accgttcgtt gcttgaccat caggtctgac cacgcatcca aggtgacatc
     2941 cccgccaact cctacaaacg caattcggcc ccagttggaa gtgcagcgta ccgctgcttg
     3001 ccgggcagcc gccacgcccg cgcagtcgat ggcgacagta gcgcctaccc catgtgtcca
     3061 ctgcaggatg gcttcaaccg gatccgcagt cttcgagttg actccgtcca ctgcgccgaa
     3121 cgtcttggcc tggctaactc tctcgtccga aacgtcaaca gcaaaggtgc gaacggccat
     3181 ggcgcttgcc agctgaaccg ccgattgccc gaccgggcca agcccgaaga cagccagagt
     3241 gtcgcgcccg ctgagctgaa cccgttccag tgctgcaaac gctgtcccag ttccgcatga
     3301 aatggccgcg ccagcagaaa aactgatatc tgaggggaga tgaatcagcg atcgggccgg
     3361 cgccttcata tacggtgcat gcccaccgtg cgccgtttgg ccaaataccg tcgcgcctct
     3421 gtcgcacatc tggacccagc cagtcctgca tggatcgcaa tactggcaac cttcgtagtg
     3481 gtagaccatc acgcgatcgc cctttcggaa cgagcgctgg tcaacgttct tgcccacttc
     3541 agcgacgatc ccgcacggtt cgtgacctgc gatgatcgga tcttcgacgt tcagtttccg
     3601 ttctgccaga aactcggggc tcttggccct caacgatgcg ccgcgcaccc cgcggtaatg
     3661 atggagatcg cttccacaga atcccgacgc cttcatctcg atgatgacct catcatcgcc
     3721 tggaacaggg tcttcaaagg ccaccagctc gagatttcgg tgtccggtga atacgacgcc
     3781 gcgcatggga gtgtccttgt cctcgaagcg tgatgggcta attcagggaa atcgcagtcg
     3841 cgtcatacca gcgcataccc atcgcgcctg ttgaacacgg tacgtaatcg tccgtcgctt
     3901 cgagcgcgat gtacagttgg tttttctttt ggccccggta agaagcgcta aatagatcgt
     3961 cctgcaggca ccggtctcgt tgaccgaaac gaggcactcg cgccgaacga gtgtcgcggg
     4021 tcagttgcgc ttacccggcg ctaagaaaaa gcgtctgtac atcgttggtc cgttcgaaaa
     4081 aaatgcacaa aacacaagac agagggtgat ctcgatggga aagctggatg gcaaggtggc
     4141 tttggttact ggtgcggcga ggggtatcgg gagagaaatc tcgctgaccc tggctcggga
     4201 gggggccgat atcgtcgcca atgccttgaa tgcgccgaac ctggaaacgc tgaaggcgga
     4261 agtcgaaaaa cttggccgag aggcgatcac tgcaattgca gacgtttcca agaaagcggc
     4321 agtcgaggcg atggtcgacg ctgcgatccg cgagttcggg aagatcgata tcctcgtatg
     4381 caacgccggt atcacccgct ttgcgccatt cctggaaatg actgaagaag attgggacat
     4441 cgtcgtcgac gttgatctca agggcacctt cttgtgcggg caggccgtcg ccaagcacat
     4501 ggtgaagcgg aaatacggga agatcgtcaa catctcctcg gcgtctgggg tcggtgcgcg
     4561 aaaccacacc atggccaact atgcggccag caaggcaggt gcgatcaatc tctcaagagt
     4621 catggcgctg gcgctcggcg attatgggat caacgtaaac gtcgtggctc ctggcgttat
     4681 caaaaccgag atgggccttt cgcggagaac gccggaggaa tgggcggctt atctggagct
     4741 ttacaaacaa caaacggcgc tgcatcgggt gggggaggct aaggacatcg ccaatgccgt
     4801 actgttcctg gtctcggatg attcatcctt tattacaggt caggtgatct tgagcgacgg
     4861 cggcctgaaa gacggtcttc tgcgctgaac ggagctgagc actgcggatt gtgggttttc
     4921 gagttcagct tagcttacgc tgcgctaaga aaagtcgtct ggacctgccg gtggagtctc
     4981 ggcacagtct tgccaagtag caggcggcag tttcgccgcc aataaatacg aagcgcgcct
     5041 aggcgttccg catttcaacc ctaaagagga ttaaatgaac ccgattcgca ggtggctgtt
     5101 tgccatcttt attgcgagcc tgatatttcc ggcggttacg tccgcgcaga cctggcccga
     5161 caagccggtg cacatgacga tgccgttccc gccgggaaca ggccccgata ttgtcatgcg
     5221 aactgtctcg gaacgtctgg ccaaaatgtg gaagcagcca gtgctcgtcg aaaacaagcc
     5281 tggcggaaac gggttcatcg ccatgtcggc cgctaagcgg gcgcctgcgg acggctacac
     5341 gctggtcatg gtcgattatg gaatcatgac cgtactgcca tacctgttcc ccaagacgat
     5401 tccatacgac ccggtgaagg acttcgacgc ggtggcgccc atgtactggg cgtattggtt
     5461 tgtggcagtc gccggcgact cgaggtttac aaagatcccc gatcttgttg ctgaggcgaa
     5521 cgccaagaac ggcaccatga cttatggatc gtcgggtgtt ggcagcccaa tgcatcttca
     5581 ggccgcgatg ctggagaacg ccacgggtac gcacatgagc cacatcccgt acaaggaaac
     5641 gcctcaggtc cttatcgata tcagccgtca cgacattggc tgggcgttta cgacgggcgc
     5701 aacgggagga gggatggcca aagggggcaa gatcaaattc ctcgccgtcg cctcgcccaa
     5761 gcgtcacccg ggatttcctg atgttccgac gatggccgaa gccggtggtc cgaaagactt
     5821 tgacctgcgt acttgggtag gactttacgc gccacatggt gtccccaagc cgatcctgga
     5881 caagatcagt gccgacgttg ccaatgttct atcggatccc gaggttaagg acttgcttgt
     5941 tgctaacgga ctcgaggcgt ggccgggccc agcatccgaa ctggtcaagc agcaagccga
     6001 ggattccaag aagtacggtg aactgaccag caagttgaag atttcgctcg attgaggcat
     6061 gactcaaggg gatgggcggt tcgcggatcg cgcgacttgc ccgtcgcccc ttgaatgagc
     6121 tgcctattgc ttgtcaatgg atcatttgaa ccctagcggt cccggctgga ctccgcctag
     6181 gcgggtcgac ggaaaagtcg ccgtcgtctt cggcgcgggg caaagcccgg gcgaagggct
     6241 tggtaacggc agagccgccg cgattcgata tgcgcgagag ggcgccaagg tactcgccgc
     6301 taatcggtcg attgaatcgg cagaagcgac agcggagctt atccgtgcgg aaggctttga
     6361 ctgcgttgct gttgtggccg acgttcgccg ggagtcggac attgccagcg ctatcgatga
     6421 agcgcagcgc cgctggggca ccgtcgacat actgcacaac aatgtagggg tgagtctcgg
     6481 tggaggtgac accgacgttc tcaacatcac cgaggaagcg ttcgacaata tctaccaaag
     6541 caatctgcgc ggtaccgctt tcgcatgcaa gtacgcactg cccattatgc gcaagcagcg
     6601 atcaggcgcc atcgtcaaca tatcgtccgc cgcggcggtc ggtctctacc cgtacgtggc
     6661 ctacaaggca acaaaagcag gcgttgttgc gctgaccgag cagctcgcgc ttcaaaacgc
     6721 gccgtacggc atcagggtca actgtatcct tcccgggctg atcgatacgc cgatggcggt
     6781 agagacaagg tccaaaaaat ggaatcaacc gcgtgatcag gtgcttgccg agcgagttgc
     6841 gaagataccg ctcggccgcc aaggcaccgg ctgggacgtg gcaaacctgt cgctcttcct
     6901 ggcgtccgat gaggccgctt tcatcaccgg ggtttcgatt ctcatcgatg gcggacgggt
     6961 gctgaaccgc atctaggtct gtcctggctg tgcgacgcta tgggccccca gcatgatacc
     7021 tatgatgtca tcgtagtcgg cgccggagca gccgggcttt gcgcggcgtc gacggccgct
     7081 gccctcggca agagtgcgct ggtgctggag cacaccgcga aggtcggggg cacgaccgcg
     7141 atatccgggg gtatggtctg gattccggtc aaccggaaga tggcggaggc gggtatcccc
     7201 gactcgctgg atgcggcctg gcgctacttg ggatcgaccg tacccggttg cgagcaggat
     7261 ccgctaatgt cggcgttcat tgcgcgtgga aacgaagcga tcgacttcct cgattcgcgc
     7321 acatcggtaa agctccgacc ggtacgacgc tatcctgact attaccccga cttgcctggc
     7381 gcaaccgcgg gtggtcgagt gctcgaaccg gtgccgttcg acgggcgggc actgggcaaa
     7441 gatttcagtc ttttgcgcga tccgattcct gagttcctgc ttttcggcgg gatgatggtc
     7501 agtcgtgaag acgtgccggt attccggcgc gtgggcagtt ctgtccgggc actttggcac
     7561 gtctcgaagc ttgtgggtcg ctacgcagga cagcgtttgc gggctcatcg gggtacgagc
     7621 ctggtcctag gcaatgcact ggcggcgagg ttgctcaagt ccgcgcgcga cctcggcgtc
     7681 gagattattg tcggcgcctc cgttcgccag cttgaggcca gcgagaaccg tgtcaccggg
     7741 gtcagtctag agatcgcggg ccgggccacg aaactacaag cacgctgcgg cgtcatcctg
     7801 gcgaccggcg gtatctccca ctacccggaa ttgcggcgtg cctatgttcc cgccagcgct
     7861 ggcaatctct ccgccacggt ctcatcaggg gcggcgtccg gcggggtcca gcttgcaaag
     7921 gaactaggag cggatgttag tccgccctcc gcaaatcttg agcgcgcctt ggcatggtgg
     7981 gtccccgtat cccgattcca acgctccaat ggggcgaccg cggtattccc ccatgtcgtt
     8041 actgaccgcg caaaaccggg ccttattgcg gtgaacaagg aggcccgacg cttcgtcaac
     8101 gaagctgtct cctaccacga gttcgttcgc gctcagctac gtgaccccga gcgctcaatt
     8161 cctgcctggc tcgtatgcga tagtcgattc atgtggaagt atgggctcgg gcgagttcgc
     8221 ccgtttaccc tttccctgcg cgccgaggtc ggcagcggat atctcaaacg agccaggtcg
     8281 ctcgacgagc tggcgaagtc catcggcctg ccggtcgccg aattccgcaa tacggtccag
     8341 aggtacaacg agagcgccag gattggtaaa gacccggagt acggtcgcgg ctcgaacatc
     8401 taccagcgtc acctaggtga tgctgatcac cacccgaacc cgtgtgtggc tccgatcgag
     8461 aagccacctt tctatgcggt tgaagtattt cccgcggacc tcggcatggc cgcaggcatt
     8521 tcaaccgacg agtacggccg agtgcttcgt gctaatgagc ctgtgcatgg actgtatgcg
     8581 tgtggcaacg acatgcattc cgttatgaac ggagcctatc ccggccccgg aatcacactt
     8641 gggcccgctc tcgtcttcgg ttatgttgcg gcatttcacg cgtgcgccga gcgaacctga
     8701 taagtccgct tgtatccgct tctgcgatcg acttcggaat cgaatccgtc cccgtatcca
     8761 gcgactcggt gtttaccagg cgcactcctc tccaaattcc ggaggagacc agatcgttgg
     8821 tgatgttctt cagctcggct cgtacggtcg atgcggtggt cgaaattttg tctggcggca
     8881 gcgcatacag ataattcgtc cggagcatcc gcgtttgaga aatcgaaagg cagcgccagc
     8941 gccccgggaa ctccagtacc aacattgcgg acatgggttg aatggtcacc ccagcgccct
     9001 ccgcgacgta ttgcatcagt agtaacaaag agtcaatctc ggcaaccggt tctcgcaccg
     9061 tgagctgcgc ccggtcgagc tccagatcaa cccggcggcg cagcccgtgg ccggccctcg
     9121 gcaacgcgag cggcatacgt gcaatctcat tgagcgacag cgatgtcttg tcccgtggga
     9181 ccaggtcgct gtccgcggat acgagtacga acagctcctc ttccagcaag ggctccagag
     9241 tcatttcgct cgccgcgctg tggctgaaca tgatcgctac atcaagctgg ccggcgcgtg
     9301 ccatctcctc gaggtacccg gagtggagcg atacgatact gaggtcaatg cctggatgcc
     9361 tcgcgcggag ttgtcgaatc aggggcatgc caagtgccgc cgccgtcgtg ggagcgagcc
     9421 ccagcgttac acgccctttg atgtccgagc ggtcctggcg cgcgataaca acggcttcgt
     9481 caagctgacg tagcatgaac ttggcatggt gatacagggc ccttccgttg ctggtagggg
     9541 ccacaccacg tgaggagcgc accagcagcg gcctgccgac ttcctcctcc aatcgggcca
     9601 tctgctggct caaggcaggt tgcacgatga atagtcgacg agacgccttc gtcaggctgc
     9661 cgctctcgac gatttgcaca aaatatcgca gttggcggag gtccatgacc tataacctgt
     9721 aatcagccac ctggctcata acgaagatcg agatcactgc tcgacggcga cgggtacctt
     9781 aacgtccttg gtggtgcgtt taaaaaatac caggagctgc cacgcgatca ggagggcaat
     9841 cagcgcaagc agggtcccgc tgatggggcg agtaacgaag gtagaaaagc tcccacggct
     9901 cagcagcagc gaccgacgga agttttcttc catcatgggt cccaagataa agcccagcat
     9961 caatggcgcc gggtcgagct ccaggcggag gaacaggtag ccggcgagac cgaatgccgc
    10021 ggtgatgtac acgtcttcca gattattgtt gatgctatag gtaccgatgc agcagaagaa
    10081 aagtattgct gggaacagta cctggtaggg aatcttgaag accgagagcc aataccgcac
    10141 caatggcacg ttgagcacca ataggaaaac gttgccgatc cacatgctgg cgaccagacc
    10201 ccagaacagc tcggggtggc cagagatcat gttgggcccg ggctggatgc ctttgatgat
    10261 gaacgccgcc atcatgagtg ccatgacggc attttcggga atcccgatgc tcatgagcgg
    10321 tatgaagctc gtccgcgccg ccgcttcatc tgcggcagct tgcccggcta cgccttcgat
    10381 cgagcctttg ccgatctcgt ccttgtactt gctgaccttt ttgtctagcg cgtaggccgc
    10441 gaactgggca atcaccggtc cgccacccgg aaggataccc agcgccgatc ccacgacact
    10501 gccgcgcagc gcgctgggga tgatgcgctt gaactccggc caggtcggca tcaatttgat
    10561 cttgcccttg aacggtgacc gatcttcatg gccgtccaga ttttttgcaa tttccgcaat
    10621 gccgaagcag cctaacgcaa cgcttaccac gcctacgccg tccgcaagga aagggaggcc
    10681 aaacgtgtag cgcagcacgc cgctgttgac gtccgtgccg atctgcccaa gaaggacgcc
    10741 gatgaagcac atcgccaggc cgttgagcag gctcccagtc gtcacgaagc tgacacagaa
    10801 gaagccgaag agcatcagcg ccgtatagtc agccggcccg aacatcaacg cgacttcgcc
    10861 cagcgccggt gccagcgcag caagcacgac gatggcgacc gtcccgccga tgaaactcga
    10921 tacacccgcc gtgaatagtg ccagaccggt cctgcccttc agagtcatct gatacccgtc
    10981 gatgcaagcg acgatactgc tcgcgtgcgg gatcttcatc gtgatggcgc ttacactgtc
    11041 gccgtactgt gcaccgtagt agataccggc cagcatgatt agtgcgccag ttactggaat
    11101 tgagtaggtc aatggaagga gcaggctgat cgttgccaag gggccgaggc cgggcaatag
    11161 accaacaagc gtgccgacga cgcaaccaat cgcacagaac aacaggttct gcaacgtcag
    11221 tgcgtgctcg aagccaaatg ccaggttgtg gaggatgtcc atcagaagag gggcaactgc
    11281 agcccgagca gttgttggaa gcccaaggcg atcgccacga ggacggcggc gaccacgagg
    11341 ttgcgtttga tcgaataggg gggatcagcg agcatcgcga agaagacgag gaacacgatg
    11401 ccaaggatca tgttcacgaa ttccgagagc agcgcgaagc cgcacagact gagcaggatg
    11461 attgcgatgt tcttgaattg aaactgcatc ggttggcgtt cgatgagcaa cgatcgaatc
    11521 acggtcgtaa tgccgatcag cagtaagaac gaactgacga ccagtgggaa cagtcccgga
    11581 ccggctcgct cgaattgccc gatcgggtat cggaaagcgc ccaatccaaa ccccagtgcg
    11641 atcgctgtga gtgccactcc tctcgcaaga ttctggttca taagactcct ccagttatct
    11701 ccggcaattg gccgggaatc ctctctatat caagcaacaa attagcattg cgatgtcgcc
    11761 acgggctata cgaacaactc tccgtgtcga tccagaagtg ccttgatcgc ttcttcactt
    11821 gcgtccgggc cgagcacctc gctggagggg aatccaacga agttgagaag gcgcgagccg
    11881 taaagcatcg ctgttatctg catggcagtc ttctcactct tcgctcaact ggagatcact
    11941 tggtttgcgg cgttcgaccg accacttgag caaggctgcg gcccggtaca cgccgtgggc
    12001 aaactttccg tacggaaggg tcaggaacaa cgccatgaca gtgccgaggt gcacgctgag
    12061 cgccagcggc aaggcaggcg ttgccttaac cagcataagg acgatgccgc tgagcgcggt
    12121 cagaaagagc aacgcgatga agccgcgatc catgggccgc tgcgccgagt cctgctgcag
    12181 cggatgtcgg cggagattca gccagaacaa tcccgcggtc ccgatcagga gcgccactcc
    12241 cccgcttagg ccaagaagtt ttggaagggc tgtatatgca tatggagcgg accagccaag
    12301 cacgtagtga tagagggttg ccacgcttgt tgccgcgaag cagaggagaa atccatagaa
    12361 ggtgaagtga tgaaacaatc gccgtcgttc ggtaaagcgg tcgttctcgt cgttgcaccc
    12421 atctccgtgc cccccgtcca agtatttcag cgtcaatatg ttgccaatgg cttcgccgac
    12481 ggcaccctgg gtcggctcac cagggcgttc gtcctgccag aaccgccagg ctccaatcgc
    12541 cagcgcaagc gcggcgaaca ggaacaccgg gccgaacatc gcaaccatga gattgtgtgg
    12601 gaataccgcg tagaagttgc cagcgggcac cgattgccag agcgggccgt ggatcgctac
    12661 agcgaggatc agaaaggcag ccagtgctcc tgcgagcgcc agggcgatcg tgagtccgtt
    12721 tctcctgtag agcgcgccaa gcgccggagg ccaggcgtac tccgtataag tgcccagccg
    12781 taccttggcc atggcctgcg gaacattgac ggcgaactca tgcgggggcg catattggca
    12841 agcgtgcagg cacgcaccgc agttgtgaca aagatttgca aggaaattca catcgccttt
    12901 gccaaaggtc aggcgccgag tcatcgccgg aaaaacggcg cagaagccct cgcagtagcg
    12961 gcacgcattg cagatctgaa ggacgcgcgc cgcttccgtt tcatcgccat tgagcgactg
    13021 actggcgatc ggaattacac gcgcactccc gcgcgccaag gcagccgcct ggcgcgtgag
    13081 tccgtcgagc gactgcacgg ggggttggct ttcaagcggc ggcacgcgac gtctccttga
    13141 gcgctgaatt ggccgcctgg gtccccgcga ttcggccaaa agccgttccg atgctcatgc
    13201 cgacaccggc ggtgtagccc tttccgagga cgttgccggc catcatttcg ccagccacga
    13261 agagattagt gctggcacgg ccggagaagc gaaccgcagc cgtttcgtcg acgcgaagcc
    13321 ccagataagt gaaggtaacc cccggcttca ccgcgtatcc gtaaaacggc ggcgtatcaa
    13381 tcgggcgtgc ccagtgcgtt ttctggatag ccagaccggc ggtccggcaa tcgtcaagag
    13441 cggtgtgatc aaatgtcccg ggacggcagg cggcgttgta ttcgttcagc gtctgaacga
    13501 accgaccttc gtcaaggccc agcgcgcgcg ctagctcagg aagcgtcccc gtcctggtac
    13561 cggggaagac cggaggcatg aaacggccga ccgccttggc gtcgataatc gagtacgcaa
    13621 cttgtcccgg ttgttgcgcg accaagcgcc cccagatcgc gtaccgcttc ggccaaaagt
    13681 cctcgccctc atcgtagaac cgcttcgctt cccggttcac gactacaccc agcgacacgc
    13741 aatcgatacg ggtacaaatg ccgccgtcgt agagcggagc tcgcgcatca atggccacca
    13801 tgtgcgcttg cgtcgggtcg ccgattgtgt cggcgcccgc atcgatcatg aaccgcaaca
    13861 attcaccggt gttgaacctc gttccccgga taagaaagtt gtcggcaggg tattcgccgc
    13921 gctcattgac gcc
//
LOCUS       DASPUL010000041         4659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_965730, whole genome shotgun sequence.
ACCESSION   DASPUL010000041 DASPUL010000000
VERSION     DASPUL010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965730"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1285
                     /locus_tag="VEJ00_00945"
     CDS             131..1285
                     /locus_tag="VEJ00_00945"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXW99752.1"
                     /translation="MRKHFALILFALLALGLCVPPVFAQASGTVKGACKDAQGNPLAD
                     AIVLWANTENGQKYTLKTNKKGEYFSLGISPGKYNVTLYKNADDQKAGKEADHANGFQ
                     VQLDENNLDFDLKKEQENQAKGVGLTPEQLKQMQEQQAKQQKEQNTVKSLNDKLNAAK
                     TAAEAGDFDTAIAQLNDANQIDPSRDLIWFKLGDYYRLSATKQTDTAEKQKRYGSAID
                     AYQKAIQLKQAAPADKDPTATAKALAAYYNNLAEAYAKSNKVDDAVKTYALAAQQDPS
                     AASQYYFNTGAVLTNAGKVDDAIAAFDKVIAADPNKADAYYWKGVNMIGKATLQGDKM
                     VAPPGTAEAFQKYLELQPTGTYAEPAKQMLTSIGASVETSFGNKKKPPKK"
     gene            1485..2630
                     /locus_tag="VEJ00_00950"
     CDS             1485..2630
                     /locus_tag="VEJ00_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HXW99753.1"
                     /translation="MASPTIAPISVALENVSGSGLVATPALLGAMLRAAEKISDLIFS
                     PGRPPQVQVYGQMIPVQVPGLSSLTADDTRHIAADLIGDNKQAITTLREHGACDISYG
                     LPGLARFRVNIFIQRGSCAVVMRVIPTAIPDFDSLRLPKQLSEITKLRDGIVLVTGPA
                     GSGKSSTLAVLLDCINQEKYYHIITIEDPIEFLHNHKCSTIHQRELHSDTPSFAHALR
                     AAMRQAPKVILVGEMRDRETIEIVLEAAETGHLVFSSLNTMDASKTVERIVSSFSPAE
                     QHSVRDRFAKSFRYIICQRLIPKSDRSGRVPAFEVLKANARTRACIEKGEREDKTLLD
                     AMKGGSSEGMQYFDGEIAALVRDRVIDLETGLSFASNPAVLGQGLAR"
     gene            2758..4017
                     /gene="glp"
                     /locus_tag="VEJ00_00955"
     CDS             2758..4017
                     /gene="glp"
                     /locus_tag="VEJ00_00955"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HXW99754.1"
                     /translation="MSDASESTVLSFEDARQVVEEQAAKLRPGPTESADLLAAAGRVL
                     AEPVRADRDIPPFPRSTRDGYVVHSADLFRLPATLAVIAEIKAGEKLERIPENVARGQ
                     AAAIMTGAPVPAGPDAVVMVEYTARLGDRVEITRGVAPGENIVPRGAEARQGSLLLDS
                     GLRLNDAAIALAASVGKSRLEVYRRPKVAVLATGDEIVDVNKTPGPTEIRNSNSYSLA
                     VQIRHSGGEPVLLPIAPDEPQALRRLIEEGLKCELLLMTGGVSMGRYDLVEQVLSEMH
                     AEFLFTGAKIQPGRPVVFGRCGAGAPAREKPVTYFFGLPGNPVSTMVTFELFARPMLE
                     ALSGMPLREPQFVHVKLKTEVRVKPGLRRFLPAILSGEYENSQVELVPWQGSGDIAAT
                     ARANCYAVISPDVERISAGEWIAVMLR"
     gene            4081..4554
                     /gene="moaC"
                     /locus_tag="VEJ00_00960"
     CDS             4081..4554
                     /gene="moaC"
                     /locus_tag="VEJ00_00960"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HXW99755.1"
                     /translation="MPRKLSHYDGAGRARMVDVSGKSATRREAEASAFVAMKPEVLTA
                     LPKNPKGDPMEVARLAGIMAAKRTAELIPMCHPLPLALVDVDVRLCENGVAIASKVAT
                     TAETGVEMEALVAAAVSALTVYDMCKGADKGIEIREIVLERKSGGKGGGYRRAKK"
BASE COUNT          988 a         1464 c         1324 g          883 t
ORIGIN      
        1 agtcgcgacg atctggcctg cggggtgtgc gacagttgcg ttttacgcct gcgggcgttc
       61 gaggcggcag gagtaagaga tccaatccca tacgcggcgc gttaggagtc gaatcgcgcc
      121 gggaggcaat atgagaaaac acttcgcact cattcttttt gcgttgctgg cgctgggact
      181 gtgtgtgcct ccggtctttg cccaggcttc aggaacggtg aagggcgcgt gcaaagatgc
      241 gcagggcaac cccctcgcgg acgccattgt gctctgggcc aatacggaaa acggccagaa
      301 gtacacgctg aagaccaaca agaagggcga gtacttctcg ctcggaatct cgccgggcaa
      361 gtacaacgtc acgctctaca agaatgccga cgatcagaag gccggcaagg aagcggacca
      421 cgccaacggg ttccaggtac aactcgacga gaacaacctc gactttgatc taaagaagga
      481 gcaagagaac caggcgaaag gagtcggcct cactcccgag cagttgaagc agatgcaaga
      541 gcagcaggcc aaacagcaga aagagcagaa caccgtaaag tcgctgaatg acaaactgaa
      601 tgctgccaag accgcggctg aggcgggcga tttcgacact gcaatcgccc aactgaacga
      661 cgccaaccag atcgatccct cccgcgatct gatctggttc aagctgggcg actactatcg
      721 tctctctgcg accaagcaga ccgacaccgc cgagaagcaa aaacgctacg gctccgccat
      781 cgacgcctat cagaaggcga tccaactcaa gcaggccgca cccgccgaca aggatccgac
      841 cgccaccgcc aaagcgctcg ccgcttacta caacaacctg gccgaagcct acgccaagtc
      901 caacaaggta gatgacgcag taaaaaccta tgccctcgcg gcccagcagg acccgagtgc
      961 cgcgagccag tattacttca acaccggtgc ggttctgacg aacgccggca aagtcgacga
     1021 cgcgatcgcg gcgttcgaca aggtcattgc cgccgacccc aacaaggcgg acgcctacta
     1081 ttggaagggc gtgaatatga tcggcaaggc cacattgcag ggcgataaga tggtggcgcc
     1141 ccccggaaca gccgaggcgt ttcagaaata tctggaattg cagccgactg gaacttacgc
     1201 cgagcccgcc aagcaaatgc tgaccagcat cggagcgtcg gtcgagacca gcttcggcaa
     1261 taagaagaaa ccgccgaaga agtagatcgc cttctggtac aagcagttgc aacgttcagc
     1321 ctagggcggc ctaccggccg cccctacttt ttattcctgc gcacgttact aaagtctacg
     1381 tacttgctgc ccttcgcagt gtccgcgtga acaggttaca gttaatacca gcgcttcttc
     1441 ctcccttcct gtggtgtgtc caattcctga ccattgagga gcgaatggca tcacccacca
     1501 ttgcgccgat ttcggttgcg ctggaaaacg tttcgggaag cggtctcgtc gccacaccgg
     1561 cattgctggg agcgatgttg cgcgctgccg agaagatcag cgacttgatt ttttcccccg
     1621 gccgcccacc ccaggtgcag gtctacggcc agatgatccc agtgcaagtg cctggactgt
     1681 cgtccctgac cgccgatgac acacggcaca tcgccgctga cttgattggc gacaacaagc
     1741 aggcgattac cacgctgcgc gagcatggcg cgtgcgacat ttcctacgga ctgccggggc
     1801 tggcgcgctt ccgcgtgaac attttcattc agcgcggcag ctgcgccgtg gtcatgcgcg
     1861 tcatcccgac ggcaattccc gacttcgact ctttgcggct tcccaaacaa ctgtccgaaa
     1921 tcaccaaact gcgcgacgga attgtgctgg taactggtcc tgccggttcg ggcaaatcgt
     1981 ccacgctggc cgtccttctc gactgcatca accaggaaaa gtactaccac atcatcacca
     2041 tcgaagatcc cattgagttc ctgcacaatc acaaatgctc gaccattcat cagcgcgaac
     2101 tgcacagcga cactcccagt tttgctcacg cgctgcgcgc cgccatgcgc caggcgccaa
     2161 aagtaattct ggtcggcgaa atgcgcgacc gcgagacgat cgagatcgtc ctcgaggccg
     2221 ctgagaccgg ccacctcgtg ttctcgagcc tcaataccat ggacgcgtcg aagacggtcg
     2281 agcgcatcgt cagctcgttc tctcccgctg agcagcactc ggtacgcgac cggttcgcga
     2341 agtccttccg ctatatcatc tgccagaggc tgattccgaa gtctgaccgc agtggccgcg
     2401 ttcccgcctt cgaagtcctg aaagctaatg cccgcacccg cgcctgcatc gaaaaaggcg
     2461 agcgcgaaga taagactctt cttgatgcca tgaagggcgg ctcgtctgag gggatgcagt
     2521 acttcgatgg cgagatcgcc gccctggttc gcgatcgcgt gatcgacctc gaaaccggct
     2581 tgtccttcgc cagcaatccc gccgtcttgg gccagggact ggctagatag cgtcctctgg
     2641 aaccgtttcc cgagttgtca ttcctcgcga agcgaggaac atcgtcttcg tatcccctga
     2701 cggacacgtt tttccaccat agttcgaccc agctctctcc tgtcgtatca tctccccatg
     2761 tccgatgcct ccgagtcgac cgttctgagc tttgaggatg ctcgccaggt agtcgaagaa
     2821 caagccgcaa agcttcgtcc cggccccacg gagtccgccg acctccttgc cgccgccggc
     2881 cgtgtcttag ccgagccggt acgcgccgat cgcgacatcc ctcccttccc acgatccacg
     2941 cgcgacggct atgtcgtgca ctccgcagat ctgttcaggc ttccggcaac gctcgcggtg
     3001 atcgccgaaa ttaaggcagg agaaaagctc gaaagaattc ctgaaaacgt cgcccggggt
     3061 caggcagccg cgatcatgac tggcgcgccc gttcccgcgg gtccggatgc ggtcgtgatg
     3121 gtcgaataca ccgcgagact tggcgatcgg gttgaaatca ccaggggagt ggcaccaggc
     3181 gaaaacatcg tcccccgcgg cgctgaggcg cgacaaggga gccttctcct cgattccggc
     3241 ctccgcttga atgacgctgc catcgcgctg gctgcctccg tcggcaaatc gcgcctcgaa
     3301 gtgtatcgtc ggccaaaagt tgcagtgctc gccactggtg acgagattgt ggatgtcaac
     3361 aaaactcccg gcccaaccga gattcgcaac tccaacagct attcgctcgc tgtccagatt
     3421 cgccactccg gcggcgagcc agtcttgctg cctatcgcgc cggacgaacc ccaggccttg
     3481 cgcagactta ttgaggaagg gttgaagtgc gagctgttac tcatgacagg cggtgtttcc
     3541 atgggccgct acgacctggt cgagcaggtt ctgtcggaga tgcacgccga gttcttgttc
     3601 accggcgcaa aaatccagcc cggacgcccc gtcgtattcg gtaggtgtgg cgcgggcgct
     3661 cccgcccgcg aaaagccggt cacctatttc tttggcctgc ccggaaaccc cgtttcgacg
     3721 atggtgacat tcgaactctt cgcccgcccc atgctcgaag ccttgtccgg gatgcctcta
     3781 cgcgaaccgc agttcgtcca cgtcaaattg aagaccgaag tccgggtcaa gcctggtctt
     3841 cgtcgctttt tgcctgcgat cctctctggc gagtacgaga actctcaggt cgaactcgtg
     3901 ccctggcagg gctcgggaga cattgccgcc acggcccggg caaactgcta tgcggtgatc
     3961 tcgccggatg tggagcgcat ctccgcaggc gagtggatcg ccgtcatgtt gcggtagtcg
     4021 cctgcgcgga cggccttagc gagtcctcgc atccactctc ctgtaagata gagctacttc
     4081 atgcccagga aactctccca ctacgacggc gcagggcgtg cccgcatggt ggatgtttcc
     4141 ggcaaatctg cgaccagacg tgaggccgaa gccagcgcgt ttgtcgccat gaaacccgag
     4201 gttctcacgg cgttgccgaa gaaccccaag ggtgatccca tggaagtggc gcgcttagcc
     4261 ggcatcatgg ccgcaaaacg cactgcagag ctgattccga tgtgccaccc tctgccgctc
     4321 gccctcgttg atgtcgatgt gcggctgtgc gagaatggcg tcgcgattgc ctccaaagtc
     4381 gcaaccacgg ccgaaaccgg cgtggaaatg gaagctctcg tcgccgccgc cgtctctgcc
     4441 ctgaccgttt acgatatgtg taagggagcc gacaagggca tcgagattcg cgagattgtg
     4501 ctcgagcgga agagcggcgg caagggcggc gggtatcgcc gtgcgaagaa atagcttcac
     4561 ctaagttcat gagcaacaac gtcaagctgc tggtggtcga cgacaatccc atggttctcg
     4621 ggatgctgca gcaagctctt gcgcccctcg gccaggtgg
//
LOCUS       DASPUL010000042         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1513792, whole genome shotgun sequence.
ACCESSION   DASPUL010000042 DASPUL010000000
VERSION     DASPUL010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513792"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VEJ00_00965"
     CDS             complement(<1..820)
                     /locus_tag="VEJ00_00965"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXW99756.1"
                     /translation="MILLSFVSALSQSDDAGQTMRPVIRGRHAAVASMKAEATEAARR
                     ILDAGGNAFDAAVGGQAALGVTDFSLNGMGSDAVLLVYDAREKKVYSINAEPRAPKLA
                     TIEWYEKNNGGKIPESDGLLSGGLPGVVDAWYTLLDRWGTMSFEQVLQPAIDLAENGF
                     PLSERGASYIAGSTKILKYPTTVKVYLPNGRAPHAGEILRNPDLANTLKKLVEAEKAN
                     QSKGRHEALKAARDRFYKGDIARDLAAFSEANGGLFRYVDFAEYTAEVETPVSIN"
     gene            complement(923..1048)
                     /locus_tag="VEJ00_00970"
     CDS             complement(923..1048)
                     /locus_tag="VEJ00_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99757.1"
                     /translation="MAREDGGSRTKIAQSLEDRSSRTKIAAVARGTVEERRFSAA"
     gene            complement(1202..1378)
                     /locus_tag="VEJ00_00975"
     CDS             complement(1202..1378)
                     /locus_tag="VEJ00_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99758.1"
                     /translation="MNRLIQAVRIVLAALREIFDEAAYTRFLRRRQMQSSRRAYAEFW
                     REREAAQARRPRCC"
     gene            complement(1375..>3174)
                     /locus_tag="VEJ00_00980"
     CDS             complement(1375..>3174)
                     /locus_tag="VEJ00_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HXW99759.1"
                     /translation="VTNKWVVFGHHFAAIAGPGPLVGPVLAAQFGYLPGTLWVLAGAV
                     FAGCVQDFVILLFSVRRDGKSLTEMAKEEIGRVGGFVAYAAVIAIIIILLGVAALIVV
                     NALKDSPWGTFTIAMTIPIALLMGLYLRRIRPGKVLEVSILGFLLVVLAIWGGQWCSL
                     HPAMARIFTLRAPTLAILIIIYGFAASALPVWLLLAPRDYLSTFVKLGTIALLAVGIV
                     ALHPTLHMPALTRFIDGTGPVFAGKIFPFAFITIACGAVSGFHSLISSGTTPKLIARE
                     PETRMVGYGAMMAESAVAIMAITAACVLQPGVYFAINSPAGVVGQTAAAATATISSWG
                     FPVTAADMQALAHAVGEQTLFNRTGGAPAFAVGMAHIFSQSLGGETVMALWYHFAVMF
                     EALFILTILDAGTRVARFMVQDALGHIYAPLGRTSWYPSILATSALIVGAWGYFLWQG
                     VKDPLGGINSLWPLFGIANQLLATVALCVATTIIIKMHRARYAAVTLIPLIWLVAVTF
                     TASWHKVFDPNPRVGFLSQAQALAAGPATSATARLIFNNRLDAAVTGILVVMVALVLI
                     ESIRQWMGVLSGSREAKVKEAPFVMTRLAEGEV"
BASE COUNT          618 a          957 c         1052 g          547 t
ORIGIN      
        1 agttgatgga aaccggcgtt tcgacttcgg cggtgtattc ggcgaagtcg acatagcgga
       61 acaggccgcc gttggcttcg gaaaaggctg ccaggtcgcg ggcgatgtcg cccttgtaga
      121 agcggtcgcg cgcggccttc agggcttcgt gccggccttt ggactgattc gctttctctg
      181 cttcgaccag tttcttgagt gtgttggcca ggtcaggatt cctgagaatc tcaccggcgt
      241 gcggagcgcg gccgttcggc aaatagacct tcacggtcgt agggtacttg agaatcttcg
      301 tcgaaccggc aatgtacgac gcgccgcgct cgctgagcgg gaagccgttt tcggccagat
      361 cgatggcagg ctgcagcacc tgttcgaagc tcatggtgcc ccagcggtcg agcagcgtgt
      421 accaggcatc gaccacgccg ggcaggcctc cggagagcag gccgtcgctc tcgggaatct
      481 tgccgccgtt gtttttctcg taccactcga tcgtcgccag cttgggcgcg cgcggctcgg
      541 cgttgatgga atagaccttc ttttcgcgcg cgtcgtagac gaggaggacc gcgtcgctgc
      601 ccatgccatt gagggaaaag tcggtgactc ccaatgcggc ctggccgccg acggcggcgt
      661 cgaaggcgtt gccgcccgca tcgagaatgc ggcgggcggc ttcggtggcc tcggctttca
      721 tggaggcgac ggcggcgtga cgcccgcgaa tgacggggcg catggtctgg ccggcgtcat
      781 cgctctggct cagggctgag acgaatgaga gaaggatcat ggaggcgaca cctgagaagg
      841 atttgaatga catggctcga gtcctcggag ataagaaaca tcgtgcgacc accagggcta
      901 aagccctggc tcgtttgcgg acttacgcgg cgctgaagcg ccgctcttcc acagtgccac
      961 gggcgactgc ggcgatcttc gtgcgactgg agcgatcttc gagcgactgc gcgatcttgg
     1021 tccgactgcc gccatcttca cgcgccatgg gcgatctccg tgcgacagta gcgaccttcg
     1081 tgcgattgcc gccatcttct cgcggcagcg gcgctctcag acagcggcag tcaacctctc
     1141 aggcgctcta cgcaaagtgc cggcgccctg taacacaatc tggcaacgcg gtaacttcac
     1201 tctagcagca ccggggccgg cgggcttgcg ccgcttcccg ttcgcgccag aattccgcat
     1261 aggccctgcg cgaggactgc atctgacggc ggcgcaggaa gcgggtgtac gccgcttcgt
     1321 cgaaaatctc tcgcagggct gcgagcacaa ttcgcacggc ctggatcaag cggttcatac
     1381 ttctccctcc gccagacgcg tcatcacaaa cggcgcttct ttcacctttg cctctcgact
     1441 gccgcttaac acccccatcc actggcggat cgactcgatc agcaccagcg ccaccattac
     1501 aaccagaatc ccggtgaccg cggcgtccag gcggttgttg aagatcaggc gcgcggtcgc
     1561 cgaggttgcg ggtccggcag ccagtgcctg cgcttgtgac agaaaaccga cgcgcgggtt
     1621 gggatcaaag accttgtgcc acgaggcggt gaaagttacc gcaaccagcc agatcagagg
     1681 aatcaaggta accgcagcat agcgggcgcg gtgcatcttg atgatgatcg tcgtggccac
     1741 gcatagcgcc accgtcgcca gcaactgatt ggcgatgccg aacagcggcc acagcgaatt
     1801 gattccgccg agcggatcct tcacgccttg ccacagaaaa tatccccagg cgccgacgat
     1861 cagggcgctt gtcgccaaaa tggacgggta ccaactggtt cggccgagcg gcgcgtagat
     1921 gtggccgagg gcgtcctgga ccatgaagcg ggcgacgcgc gtcccggcgt cgagaatcgt
     1981 gaggatgaaa agcgcctcga acatgacggc gaagtggtac cagagcgcca tcacagtttc
     2041 cccgccgagg ctctgcgaga agatgtgcgc catgcccacg gcaaaggcgg gtgcgccgcc
     2101 ggtgcggttg aacagcgtct gttcgccaac ggcgtgagcc aacgcctgca tgtcggcggc
     2161 ggtgacaggg aatccccaac tggagatggt cgcagtggcg gcggccgcgg tctggccgac
     2221 gacgcccgcc ggactattga tcgcaaagta gacgccgggc tgaagcacgc acgcggcggt
     2281 gatcgccatg attgcgaccg cggactcggc catcatggcg ccgtagccga ccatgcgggt
     2341 ctccggttcg cgggcgatga gtttgggggt ggtgccgctg gaaattaacg aatggaatcc
     2401 gctcaccgca ccgcaggcga tggtgatgaa ggcgaagggg aatatctttc ccgcaaacac
     2461 cggtccggtg ccatcgatga agcgggtgag cgcgggcatg tgcagcgtgg gatggagcgc
     2521 gacgatgccg acagccagca gtgcgatcgt ccccagtttc acaaatgtgc tcaggtagtc
     2581 ccgcggagcc agcagcagcc acacgggcag agcggaggcg gcgaatccgt agatgatgat
     2641 gagaatggcc aacgtaggtg cgcgcagcgt gaagatgcgt gccatcgctg ggtgcagcga
     2701 gcaccactgg cctccccaga tcgccagcac tacaagaaga aagccgagga tcgagacttc
     2761 gagaactttg ccggggcgaa tgcggcgcag gtagagtccc atcagcagtg cgatgggaat
     2821 ggtcatcgcg atggtgaacg ttccccaggg gctgtccttc agggcgttga ccacgatcag
     2881 ggcggcgacg ccgagcagaa tgatgatgat cgcgatgacg gcggcatagg ctacgaagcc
     2941 gcccacgcgg ccgatttctt ctttcgccat ttcggtgagc gacttgccgt cgcgccggac
     3001 cgagaacagc aggatgacaa aatcctgcac gcagccggcg aagacggcgc ccgccagcac
     3061 ccacaacgtg ccgggaagat agccgaactg cgcggccagc acaggtccca cgagcgggcc
     3121 cggaccggcg atggcggcaa agtgatggcc gaagacgacc catttgttgg tgac
//
LOCUS       DASPUL010000043         3858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_828776, whole genome shotgun sequence.
ACCESSION   DASPUL010000043 DASPUL010000000
VERSION     DASPUL010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828776"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VEJ00_00985"
     CDS             <1..238
                     /locus_tag="VEJ00_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99760.1"
                     /translation="YKIREGVTIQPSAGFYNLFNFANFDLPGTALNGLLTGAAGQING
                     TIPAGHNVDRVGVGTGVYSLGSPRQLEFGLRLTF"
     gene            complement(625..1815)
                     /locus_tag="VEJ00_00990"
     CDS             complement(625..1815)
                     /locus_tag="VEJ00_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXW99761.1"
                     /translation="MAARSYDIIMVGGGIAASTLAKAMAGRGASVLVLERETQFKDRV
                     RGEGVVPWGVAEANKLEICQLLKEKCAHDVSYVEAGSGLRDLRATTLQRLPLFSFPHQ
                     VMQETLLAAAANAGAEVRRGVSVKHVEGGVEPVVVVDGHSPERISARLVVAADGRDSA
                     VRKWTGFSVREQSNGYYMAGVLLTDVHASAEIFYAVFNPELGTWTGLIPQAGGQFRAY
                     FTYPKTMGYRLQGESMLNQFIRESARSYAPMADYLGDAKSIGPLASFDASDKWVEHPY
                     RDGVVLVGDAAATTDPTYGQGLSLAMRAARMLGDELTNNSDWEAAGHRYAEQHQRSFH
                     ACHVVEGWLRTVFQDPSPEASALRKRTMPLIAEDPARVPDHIFSGPDLPLNEQIRARF
                     FGEC"
     gene            2176..2805
                     /locus_tag="VEJ00_00995"
     CDS             2176..2805
                     /locus_tag="VEJ00_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXW99762.1"
                     /translation="MPKLLDDTVAKNRRKIERAAQELFTRQGFHGTTVREIAQKARIS
                     MGKLYLYYPNKEDIFIGLVHHMEQRMEALRQKELLPLMQSPDPESLKELGMAVGRFVS
                     ENLDYWRLMYVDVVEFRHKHFINSYREVAGGLRNYSTALFQKKPIPFPEDVNPGFAYV
                     NLYLQFVTYFLVEELFGARRHLGVSDEEAIEQLVRLYTRGGKNRVSKQQ"
     gene            2826..>3858
                     /locus_tag="VEJ00_01000"
     CDS             2826..>3858
                     /locus_tag="VEJ00_01000"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXW99763.1"
                     /translation="MRTRTVATFLAAIAVFCFLTPFTHGQTATTGQIVGAVKDSSGAI
                     VVGANVALTSDAGVRRDVATDTGGRYAFPLLDPGLYRLEVTMSGFATVKLQNIAVRIT
                     ETAVADVVLKVATTPTTVSVTGESPLVQTESSSRGTVIDEQQIRELPLPTRNFQQLLT
                     LTTGTSGTLQNSSELGRGDVAVYVNGQRALSNDVVINGVDANSIGTGSNPNLAVPSID
                     SLQEFIVQTSMYDASAGRNAGGNVAAVTKSGTNSLHGDLYEFLRNTDLNANNFFLNGV
                     SGLRTPRPAYRRNQFGGTLGGPIMKDRAWFFISYQGTRETNGTSLTNSLATVFLPAFL
                     GPQRDYQVVG"
BASE COUNT          819 a         1144 c         1105 g          790 t
ORIGIN      
        1 ctacaagatc agagagggag tcaccataca accgagtgcc ggcttctata acctcttcaa
       61 ctttgccaat tttgacttgc cgggtacggc actcaacgga ttgctgacag gggccgcagg
      121 acagattaac ggaaccattc ccgcagggca caacgtcgat cgagtgggtg tgggaactgg
      181 cgtctactcg ctcggttcgc cacggcagtt ggagtttggg ctgcggttga ctttctagcg
      241 ttccgcagta actggcgtct caggtgtgcc gcagaatgtg ccgtacgctt cgagccccta
      301 tgcgccaata cgggcttatc gctacttcaa caaagtgctt ccgtgcgggc taggttgtgg
      361 gcaagtgatt gatttcagat ggtgttcgtt ggtgttcgac ctgctgaggt gcgacaactc
      421 ttaatcagta ggttgaaggt tcgattcctt ccgcgctcac catctttcat tgcaagcaca
      481 ggacttaagc tgcgctctcg gtgccttcag gctgctcaga actggctgct acgcttcacc
      541 aactgcgggg tggcgtcgga gtcgccgact catcaggagt tgaaccaaac cggcgttctt
      601 gatcgactca gaggggaaat ggaatcagca ttcgccaaag aaccgcgcac gtatttgttc
      661 gtttagaggc aagtcgggac cgctaaagat gtgatcagga acgcgcgcgg gatcctcggc
      721 aatcaggggc atagttctct tccgcaatgc tgatgcttcg ggtgaaggat cttgaaagac
      781 ggtgcgcaac catccctcga ccacgtggca tgcatgaaaa ctccgctgat gctgttcggc
      841 gtagcggtgt cctgcggctt cccagtcgga attgttcgta agttcgtcgc caagcattcg
      901 cgcagcacgc atagcaaggg aaagcccttg gccgtaggtg gggtcggtgg tggccgcagc
      961 atcgccgacc aacaccacgc catcgcgata agggtgttcc acccatttgt cgctcgcatc
     1021 aaaactggcc aaaggcccga tacttttcgc gtccccgagg taatcggcca tgggagcata
     1081 acttctcgcg gactcacgaa tgaactgatt gagcatggat tcgccctgca ggcggtagcc
     1141 catggtcttc ggataagtaa agtaggcccg gaattggccg ccggcctggg gaatgagacc
     1201 ggtccatgtg ccgagctcag ggttgaacac agcataaaaa atctccgcgg acgcgtgtac
     1261 gtcggtcagc aagacaccgg ccatgtagta gccattgctt tgttcgcgaa ccgaaaaccc
     1321 agtccatttt cgcaccgcag agtcgcggcc atcggcggca accacgagac gggctgagat
     1381 ccgctcaggg ctgtgcccat caacaaccac gacaggttcg accccgccct cgacgtgctt
     1441 aacgctcact ccgcggcgca cttctgcgcc ggcgtttgcc gcagccgcaa gcagggtttc
     1501 ctgcataacc tgatgtggga aggagaatag aggtaaccgc tgcagcgtcg tcgctctgag
     1561 gtctcgcaag cctgacccgg cctcgacgta agacacatca tgggcgcatt tttccttgag
     1621 cagctggcaa atttcgagtt tattcgcttc ggccacgccc catggcacaa caccctcgcc
     1681 gcgcacacgg tctttgaact gcgtttcccg ctccaagacc aggacgctgg cgcctcgccc
     1741 ggccatcgct ttggccaatg tggacgcggc aatcccgcca cctaccatga tgatgtcgta
     1801 gcttcgcgcc gccatcccga gctccttctg acgtgactgc ctgggtttga aatgtggaat
     1861 cgaagatcga attgttgacg catcttagtg aagctgtccc taagagtcaa tcgatccgcg
     1921 aacggaacgc cggggcgtga ttgacgggca agtagaggtt agctcccaat tgcggacaac
     1981 ccacgaagta gcatggccac cagggaccgg tcatggtagc aaatctccct cccgcttgcc
     2041 gagcaacgac cgaaggtcca tgagttgtct ggaccgagat gcaaacaagt ttgaaaactt
     2101 cttgacaagg gcaaagaggc ggtcttatgc ttgccaacga gagcgagcgc tcgtcttggt
     2161 aagggcgcac cggccatgcc caagcttctg gacgataccg tcgccaagaa ccgccgcaag
     2221 atcgaacggg ctgcccagga acttttcacg cggcagggat ttcacggcac gaccgtccgt
     2281 gagattgcgc aaaaggcccg catctccatg ggcaaactct acctgtacta ccccaacaag
     2341 gaagacatct tcatcggcct ggtccatcac atggagcaga ggatggaggc cctgcggcag
     2401 aaagagcttc ttcccctcat gcagtccccg gacccggaat cgctgaagga actgggaatg
     2461 gccgtcggcc gcttcgtctc ggagaatctg gactactggc gactcatgta cgtcgacgtc
     2521 gtcgaattcc gtcacaagca cttcatcaac agctatcggg aagttgccgg tggcttgcgt
     2581 aactactcga ctgcgctgtt ccaaaaaaag cccattccct ttcccgagga cgtgaatccc
     2641 ggatttgcct acgtcaattt gtacttacag ttcgtgacct actttctggt ggaggaactc
     2701 tttggagcca ggcgtcacct gggtgtgagt gatgaagagg ccatcgaaca gcttgtccgt
     2761 ctttataccc gcggcgggaa gaaccgagtc tcaaagcagc aatgagcgcg acacgaggag
     2821 gaactatgcg aacccgaaca gtggccacgt tcctggcggc aatcgccgtt ttttgtttct
     2881 tgacgccatt cacccacggg cagactgcca ccacggggca aatcgtgggg gcagtgaaag
     2941 attcgagtgg ggccatcgtg gttggcgcga atgtcgcctt gaccagcgat gccggcgtcc
     3001 ggcgcgatgt agcgaccgac accggtggtc gttacgcatt tcctctgctc gacccgggcc
     3061 tctaccgcct cgaagtaacg atgagcggat tcgcgacggt caaactgcag aacatcgccg
     3121 tcagaataac cgaaactgcg gtggccgacg ttgttctcaa ggtggcgacg acgccaacca
     3181 cagtttccgt caccggcgaa tcgccgctgg tccagaccga gagttcctct cggggcacgg
     3241 tcatcgacga gcagcagatc cgtgaactgc cgctgccgac gcgcaacttc cagcaactgc
     3301 tgacgctgac caccgggacg tcaggtacgc tgcagaactc ttccgagttg gggcgcggcg
     3361 acgttgcggt ttacgtcaac ggacagcgcg cgctttctaa cgacgtggtg atcaacggcg
     3421 tcgatgccaa ctctatcggc acgggttcga acccgaatct cgccgtgccc tcgatcgact
     3481 cgctgcagga attcattgtg cagaccagca tgtacgacgc ctcggccggc cgcaacgccg
     3541 gcggcaacgt ggctgcggtc accaaatcgg gcaccaacag ccttcatggc gatctgtatg
     3601 agttcctgcg caataccgat ctgaacgcca acaacttctt ccttaacggg gtcagtggcc
     3661 tgcgcacacc gcgccccgcc taccggcgca accagttcgg cggcacgctg ggcggtccca
     3721 tcatgaagga ccgcgcctgg ttcttcatct cctatcaggg cacccgcgaa accaacggta
     3781 cgtcgctgac caattcgctg gccacggtgt tcctgccggc atttctggga ccgcagcgcg
     3841 actatcaggt tgtgggtg
//
LOCUS       DASPUL010000044         7824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1925433, whole genome shotgun sequence.
ACCESSION   DASPUL010000044 DASPUL010000000
VERSION     DASPUL010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7824)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7824
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1925433"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VEJ00_01005"
     CDS             <1..198
                     /locus_tag="VEJ00_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99764.1"
                     /translation="FAFFLARKLPTSVDAPQVGQQVPDFTLTDSNGKAVSLDSLFAAE
                     SGEPQAPAPKAVLLIFYRGYW"
     gene            211..543
                     /locus_tag="VEJ00_01010"
                     /pseudo
     CDS             211..543
                     /locus_tag="VEJ00_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            653..1684
                     /gene="glpX"
                     /locus_tag="VEJ00_01015"
     CDS             653..1684
                     /gene="glpX"
                     /locus_tag="VEJ00_01015"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HXW99765.1"
                     /translation="MGTQTRSEGKARSFGENIESDLALEFLRVVEIAAIASARTMGQG
                     ERKLSDEVATEAMRKTMDTIPMRGTIVIGEGERDEAPMLYIGEQVGAEFHDGRNVPEV
                     DIAVDPLEGTNLCATGAPGSITVLAASERGGLLNAPDCYMDKIVVGPSCKDAVELDAP
                     VADNLKNIARRLERDVEDLVVIVLDRSRHEKLIADIRAAGARIRLIGDGDLSAGIAAA
                     VIGTGVHAVMGSGGAPEGVITAAAIRCLNGYMLGRLVVKPDQEERLARMGIKDKNRIY
                     EAEDLAPGKQMIFAATGVTDGALMRGVRFFGEGTRTSSVIMTLHTGKVRFVESIHLEK
                     GPDVKVRFS"
     gene            complement(1694..2905)
                     /locus_tag="VEJ00_01020"
     CDS             complement(1694..2905)
                     /locus_tag="VEJ00_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HXW99766.1"
                     /translation="MKTSVLGAVASGMGAHAASPHGSGSPPSDAPKRPIIICAHNGYV
                     YVEDAYAFLKGGGDTLDAAIRVVKGPEDDPNDDSVGLGGLPNEECVVELDACCMHGPT
                     RRAGSVGGVRNIKNVSMVSKAVMEHTGHVMLVGEGAERFAVAVGFPRENLLTERSRKI
                     WLLWKETQSDRDWWGPGMADPHWQPPLESKPQSELWRDRIEQLKQRAADLGIEPEFQL
                     AAVHRVLFPPTGTIHCSALNEKGEISGVTTTSGLAFKLPGRCGDSPIIGAGCYTDQDV
                     GSAGATGSGEENIKVAGAHTIVENMRRGMSPQEAGMDALKRIVRNYNSDMNKLRFMDM
                     TYYILRKDGAYACVCLWEGYSPGHPHKMAVHDGTLHWEVGTPLLKGYSQEWPPMPVIP
                     EELKKDSVYVK"
     gene            complement(3079..4326)
                     /locus_tag="VEJ00_01025"
     CDS             complement(3079..4326)
                     /locus_tag="VEJ00_01025"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HXW99767.1"
                     /translation="MSMTREQIRELAEFQDSQSGAVTFYFQPVTPRNKAHKEDTILVK
                     DLAREALQTLEGKSAAPKVKRDSVRADIERILRLSGELRANGTHAKAVFACSAQGFWR
                     EFDLPAALPGTQLFIDRYFHLKPVAQLLGAFPRLGVVLVDRHRARFFDLRLGELTERE
                     SLFQPLSRKGRSDGYAGYDGGHAQRRVDDEARQHFKAVAEVLRQSLEKGIFERWILGC
                     QDVHRSQLEPQLHPYVAQALLGRFPADVAHTTRDEIRSHAEQIVERWQSERRRELVSQ
                     ALSQARSHAKGVTGLRRVLRSLELGEVQTLLLGESFQGHAVECSGCGHLDAHIVTYCP
                     ACGRATHDVVDVGEAILPWVIRRDIETFYVKNDPEFDRVGNVAALLRFRSNTVQPIDA
                     GVANQLAKGGAAYPGRLRRFASR"
     gene            complement(4354..5406)
                     /locus_tag="VEJ00_01030"
     CDS             complement(4354..5406)
                     /locus_tag="VEJ00_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99768.1"
                     /translation="MNVHVSYRLHKTPAVEKDIQHQIEKLRKRLQVFRPELIHLKGVV
                     EEISAREGTSVSLNLRLPSGQLAVQTKAATAPAAVKSAFEDLLQQVNKHKEILRSSHK
                     WQRGKRGNSARPGASVREVPFEQTLAAVYPATISGDDVRSYVNVNLPRLERFVEREIY
                     FREAQEVIPPDTLTKEEVIDETIAAALAEGPEKPERLALEPWLYKLALQALDDLSRTD
                     ESNDHAVPLEGSARRPNVRASDEAELQFHQPDESITGETVIGDARVATPEQIMASDEM
                     LRLIASALRDLGSGPREAFILHAVEGFTVEEITAITSHPADRVLAFISAAREHLRRAP
                     ALAKQFNGRLAPTGAA"
     gene            5733..7073
                     /locus_tag="VEJ00_01035"
     CDS             5733..7073
                     /locus_tag="VEJ00_01035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXW99769.1"
                     /translation="MTRIELLFFFFLSIRALAVAQTIPSKEVETVYPDAHTLYLDLHQ
                     NPELSGHETQTATKLATRLRSAGYDVTEHMGGTGVVAILKNGAGPTIMLRTELDALPV
                     EEKTGLPYASKVHTKDDAGHDVSVMHACGHDVHMAALLATAEIMARSKNTWHGTLMLI
                     GQPAEETIGGADGMIRDGLFTRFPKPDVAVALHVGNEYPAGVVGVTPGTFDTNSDSVR
                     ITIYGKGGHGASPHKTVDPIVIAARTILSLQTIVSREVKPGEMAIVTVGYIRAGTKNN
                     IIPDDAEMGLTIRTFKSDVRKQVLAAIARIAKAEAAAANAPREPLIEHYESTDSVYND
                     PALAEHERAPLEAALGKEHVVTAEPHTGSEDFSYFVEQGVPGFYFSLGGADPAKFAES
                     KATGVPLPSNHSSLFAPDLDPALHTGIIAEVAVLRNLLNMSPEELRKLTATPQP"
     gene            complement(7057..>7824)
                     /locus_tag="VEJ00_01040"
     CDS             complement(7057..>7824)
                     /locus_tag="VEJ00_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HXW99770.1"
                     /translation="HPDLPFQYFLDLCSGLKQHFPQVHLKAFTMVEVAYLSRRAKLSI
                     RETLEQLKASGVDSLPGGGAEIFADRVRHIICDHKIDGDQWLETARLAHQIGLKSNAT
                     MLYGHVENDEDRVDHLLKLRALQDETLGFQTFIPLAFHPDNTPLQHLPKTTGMLDIKH
                     IAVSRLVLDNFLHIKSYWQMMTPKIAQISLRFGADDMDGTVIEEKIYHDAGATTPQGM
                     RREELERLIRAAGREPVERDTLYRPVTRTETSVTVAV"
BASE COUNT         1513 a         2411 c         2390 g         1510 t
ORIGIN      
        1 ttcgcctttt tcctggctcg caagctcccc acttcggtgg atgccccgca ggtcggtcag
       61 caagttccgg atttcaccct gaccgacagc aatggcaagg ccgtttccct cgacagcctc
      121 ttcgcagccg aatccggcga accccaagcc ccggcgccga aggcagtcct gttgatcttc
      181 tatcgcggct actggtgacc ttactgcaac ctcgagttac gaggtgtcga gaaacgactg
      241 gcggacttcc aggccgccgg agtgcgtccg gtcgcgatca gcgtagacgt gccacagatc
      301 tcactcgatc tcagcaggaa ggcgggctac accttcccga ttctctccga tcctgatgcc
      361 gctgtaatcc gcagctacca tctgctccac gcgggtggag ggcctgaggg ccacgacatc
      421 gcgcgcccgg cagaatttct ggtcggcaga aacaagaccg tacgctggag caacttcacc
      481 gaagatatcc gggtgcgagc gcgggctgac gaaatgctcg ccgcggccag gcagctgcgc
      541 tgatccttgt tgaccgccga tacagaacgc gggtgacttt ccctccgact tcgattgcgc
      601 tatagtctca acccggcgcg cgattcggta tccaggcgca cgggagattc ccatgggcac
      661 gcagactcgc tccgaaggca aggcccgcag tttcggcgaa aacatcgaaa gcgatctggc
      721 gctcgaattc ctgcgcgtgg tcgaaattgc cgccatcgct tcggcgcgca ccatgggcca
      781 gggcgagcgc aagctcagcg atgaagtcgc caccgaggcc atgcgcaaga ccatggacac
      841 catccccatg cgcggcacca tcgtcatcgg cgagggtgag cgtgacgagg cccccatgct
      901 ttatatcggc gagcaggtag gggccgagtt tcacgacgga agaaatgtgc ccgaagtcga
      961 catcgccgtc gatccactgg aaggcacgaa tctgtgtgct accggcgctc ccgggtcgat
     1021 cacggtgctg gccgcttctg aacggggagg gctgctcaac gcgcccgact gctacatgga
     1081 taaaatcgtc gtgggcccgt cgtgcaagga tgcggttgag cttgacgcgc cggtagccga
     1141 caatctgaag aacatcgcca ggcgtttgga gcgcgatgtg gaagatctcg tcgtgattgt
     1201 tcttgatcgt tcgcggcacg agaaactcat cgctgacatt cgtgcggccg gcgctcgcat
     1261 tcgcctcatc ggcgacggcg atctttccgc aggaattgcg gccgctgtca tcggtaccgg
     1321 agtgcatgcg gtcatgggct caggtggcgc tcccgagggc gtgatcactg ccgctgccat
     1381 ccgctgcctg aacggataca tgctcggccg cctcgttgtg aagcccgacc aggaagagcg
     1441 tctggcgcgc atgggcatca aggataagaa tcgaatctac gaagctgaag acctcgcccc
     1501 cggcaagcaa atgatcttcg ctgcgacagg cgtcaccgat ggcgcactga tgaggggagt
     1561 acgattcttc ggcgaaggca cgcgaacttc gtcggtgatt atgacgctgc acaccggcaa
     1621 agtgcggttt gtcgaaagca tccacctgga gaaggggccg gacgtgaagg taagattctc
     1681 gtaggctgat tccctacttc acgtacacgg aatccttctt gagctcctcc gggatgaccg
     1741 gcatgggcgg ccattcctgg gaatatccct ttagcagcgg cgtacccacc tcccagtgca
     1801 gcgttccgtc gtggaccgcc atcttgtgcg gatggcctgg ggagtatccc tcccacaagc
     1861 acacgcaggc ataggcaccg tccttgcgca gaatgtagta cgtcatatcc atgaagcgta
     1921 gcttgttcat gtcgctgttg tagttacgca cgatgcgctt cagcgcatcc atgcccgctt
     1981 cctggggaga catgccgcgg cgcatattct cgacgatcgt gtgcgcgccc gcaaccttga
     2041 tgttttcttc tccgctaccg gtcgcgccgg cggagccgac atcctgatcg gtgtagcagc
     2101 ctgcgccgat gatcggcgaa tcgccgcagc ggcctggcaa tttgaaggca aggccgctgg
     2161 tggtagtgac gccggatatt tcgcctttct cattcaacgc ggagcagtga atcgtgcctg
     2221 tggggggaaa cagaacgcga tgcacggcgg ccaactggaa ttccggctcg attccgagat
     2281 cggcggcgcg ttgcttcagt tgttcaatgc gatcgcgcca caactcagac tgcggcttcg
     2341 actcaagcgg cggctgccag tgcgggtcgg ccattcccgg cccccaccaa tcacgatcgg
     2401 attgagtttc cttccacagc agccagatct tgcgcgagcg ctcggtcagc aggttctcgc
     2461 gcggaaagcc tacggcgacg gcaaagcgtt cggcgccttc gccgaccagc atgacatgcc
     2521 cggtgtgctc catcacggct ttcgacacca tggagacatt cttgatgttg cggacaccgc
     2581 ccaccgaacc ggcgcgccgc gtaggcccat gcatgcagca ggcatcgagc tcgaccacgc
     2641 actcttcgtt gggcaagccg ccgaggccga ccgaatcgtc gttaggatcg tcctccgggc
     2701 ccttcaccac ccggatggcg gcgtcgaggg tgtcaccgcc gcccttgagg aaggcatacg
     2761 cgtcctcgac atacacatag ccgttgtgag cgcagatgat gatcggccgt ttcggcgcat
     2821 cggacggagg cgatcccgag ccatgcgggg acgccgcgtg cgctcccatc ccactggcga
     2881 cggcccccag gacagaggtc ttcacgaaat cacggcgcga aaaagtcaag gagtcctccc
     2941 cagaagatca tgccggcaga aggcgaagga tatcacggcc cggacagagg cggagtgtgt
     3001 cctccggaag aattgactgt aaatcgcgga gcaaccctgc acacctatgc tcgcgactca
     3061 ctgctcattg ctcacgactc aacggctggc gaaccggcgc aggcgcccag ggtaagctgc
     3121 gccgcctttt gctagctgat tggccacgcc ggcgtcgatg ggctgcacgg tatttgaccg
     3181 gaagcgcagc agtgcggcaa cgttcccgac cctgtcgaat tccgggtcgt tcttcacata
     3241 aaaggtctcg atatcgcgac ggataaccca gggcagtatg gcctcgccga cgtccacaac
     3301 gtcgtgcgtg gcccgtccgc acgcgggaca ataggtcacg atgtgcgcgt cgaggtggcc
     3361 gcacccggaa cattcgacgg cgtgtccttg aaaactctcg cccaggagca gggtctgcac
     3421 ctctcccagt tccagcgaac gcaggacgcg gcgcaatccg gttacaccct ttgcgtggct
     3481 gcgcgcctgg ctcagtgcct gactgaccag ctcgcggcgg cgctcgctct gccagcgctc
     3541 cacaatctgc tcggcatggc tgcggatctc gtcgcgagtg gtgtgagcga catcggcggg
     3601 aaagcgcccc agcagggcct gggccacgta gggatggagt tgcggttcaa gctgcgaacg
     3661 gtgcacgtcc tggcatccga gaatccacct ctcgaagatg cctttttcca gcgactgcct
     3721 cagcacctcg gccaccgcct tgaaatgctg gcgggcctcg tcgtcgacgc gacgctgggc
     3781 atggccgccg tcgtatccgg cgtagccgtc gctgcgtccc ttgcgcgaaa ggggttgaaa
     3841 caaactctcg cgctcggtta gctcgcccag acgaagatcg aagaaacgcg cccgatgccg
     3901 gtcgaccagc accacaccca ggcggggaaa cgctcccagc aattgagcca cgggcttgag
     3961 atggaaatag cggtcgatga atagctgggt gccaggcaag gcggcgggca ggtcgaattc
     4021 ccgccagaag ccttgagcgg aacatgcgaa cacggctttg gcgtgggtgc cgttggcgcg
     4081 cagttcgcct gacaatcgca ggatgcgctc aatgtcggct cgtacgctat cgcgctttac
     4141 tttgggggcc gcacttttac cctccagcgt ctgcaacgcc tcgcgtgcca ggtctttgac
     4201 aaggatcgtg tcctccttgt gcgccttgtt gcggggcgtg accggctgga agtagaaagt
     4261 aaccgcaccg gattgtgaat cctggaattc agccaactct cggatttgtt cgcgggtcat
     4321 gctcatggat tatctcccca agaaaagatc ggactaagca gcgccggtag gagccagccg
     4381 gccattgaac tgtttggcca gcgccggagc tctgcgcagg tgttcgcgag cggcagaaat
     4441 gaatgcgagc acccggtcgg cagggtggct ggtaatggcg gtgatctcct ccaccgtgaa
     4501 gccttcgacg gcatggagaa tgaacgcctc gcgcgggccg gagcccaggt cacgcagagc
     4561 ggacgcgatc aggcgcagca tctcatcgga ggccatgatc tgctccggtg tggccacgcg
     4621 tgcatccccg atgacggttt cgccagtgat ggactcgtcg ggctgatgga attgcagctc
     4681 cgcctcgtcg ctggccctca cgttgggccg tcgcgcggat ccctccagag gcacagcgtg
     4741 gtcattgctc tcgtccgtgc gtgagaggtc atcgagcgcc tgcagagcca gcttgtagag
     4801 ccagggctcc agcgccagcc gttcgggttt ctccggacct tcggccaggg cggcagcgat
     4861 ggtttcgtcg atcacttctt ccttggtcag cgtgtccgga gggatcacct cttgcgcttc
     4921 gcggaagtag atttcgcgct ccacaaagcg ctccagccgg ggcaggttca cattgacgta
     4981 ggaacgaaca tcgtcgccgg agatggtcgc cggataaacc gcggcgagcg tctgctcgaa
     5041 ggggacttcg cgcacagacg ccccaggccg ggcgctgttg ccgcgctttc cccgctgcca
     5101 cttgtgtgaa gaacgcaaaa tctctttgtg cttgttgacc tgctgcagca ggtcttcgaa
     5161 cgcgctcttc accgcggccg gcgcagtcgc cgccttggtc tgtaccgcca gctggccgga
     5221 cggcaaacgc aggttcagtg agacgctggt gccttcgcgg gcggaaatct cctcgacgac
     5281 tcctttgaga tggatcaatt ccggacggaa gacctgcaga cgtttccgca gcttttcgat
     5341 ttgatgttgg atgtcttttt ctacggcagg agttttatga agccggtaac taacgtggac
     5401 gttcataagc gcctcggcct tgccaccgaa gtttgcgcaa ccaggtgctt cctaaaccga
     5461 attgtacacc cggtgaaaac aatgcgaacg gcgaattcgc gagaatactc tctgccgagt
     5521 tcccaaaatc gtcgcgtaga cgtctttagc ccgccggcat attgtttgcg gatcagaatc
     5581 gagttatgtt tagaagatac gacccctctt gtgtgggggt ctcttggggc cgaatgacgc
     5641 ctcgggcata gagttcagtg cccctcactc tgctcgcatt gacgccaacc ctctcgttgc
     5701 aagccgatta ggctgccgct atcatccgag ccatgactcg gatagaactc ctgttctttt
     5761 tctttctgtc aatcagggcg ctggccgttg cccaaaccat cccatccaaa gaagtcgaga
     5821 ccgtctatcc cgacgcacac accttgtacc tcgatctgca ccagaatccc gaactgtcgg
     5881 gacacgagac gcagaccgcc accaagctgg ccacccgact gcgtagcgcc ggttacgacg
     5941 tgaccgaaca catgggaggc acgggcgtcg tcgccattct caagaacggt gccgggccga
     6001 ccatcatgct ccgcactgaa ctcgacgcgc tgccggtcga ggagaaaacc ggtcttccct
     6061 acgcgagcaa ggtgcacacc aaagacgatg ccggacacga cgtctccgtg atgcacgcct
     6121 gcggccacga tgtgcacatg gcggcgcttc tggcgaccgc cgaaatcatg gcgcgcagca
     6181 agaatacgtg gcacggcacg ctgatgctga tcggccagcc ggcagaagaa acgattggcg
     6241 gagcggatgg gatgatccga gacggcctct tcacccgctt ccccaagccc gacgttgccg
     6301 tggccctgca cgtggggaat gaatatccgg ccggggttgt gggagtcacg ccgggaacct
     6361 tcgacacaaa ctccgactcg gtacgcatca ccatctacgg taaaggcggt cacggcgcat
     6421 ccccgcacaa gacagtggac ccgatcgtga tcgcggcgcg aaccattttg tccttgcaga
     6481 caatcgtctc ccgtgaggta aagccgggcg agatggcaat tgtcaccgtg ggctacatcc
     6541 gggccggaac gaagaacaac atcatccctg acgatgcgga gatgggactg accattcgga
     6601 cgtttaagtc agacgtgcgc aagcaggtgc tggccgcgat tgcccgcatc gccaaagccg
     6661 aagctgcggc ggcgaatgcg ccgcgtgagc cccttattga gcactatgaa tcgaccgact
     6721 ccgtttacaa cgatccggca ctggccgagc acgaacgcgc gccgctggaa gctgcactgg
     6781 ggaaggaaca cgtggtcact gccgagcctc acaccggttc ggaggatttc tcctatttcg
     6841 tggagcaggg cgtgccgggc ttttacttca gcctgggcgg agccgatccg gccaagttcg
     6901 ccgaatcgaa ggccacgggc gttccgctgc cgtcgaatca ctcttccctg tttgcaccgg
     6961 atctggatcc agcactacac acggggatca ttgcggaagt agcagtgctg cggaacctgc
     7021 tgaatatgtc gccggaggaa ctgcggaagt taacggctac accgcaaccg tgaccgaagt
     7081 ctccgtcctc gtcaccggcc gatacagcgt gtcgcgctcg accggttcac gcccggcggc
     7141 gcggatcagg cgctccagtt cttcgcggcg catgccttgc ggcgtggtag cgccggcatc
     7201 gtggtagatc ttttcctcga tcaccgttcc gtccatgtcg tcggcaccga agcgcaagga
     7261 aatctgcgcg atcttcggcg tcatcatctg ccagtacgat ttgatgtgca ggaagttgtc
     7321 gagcacgagc cggctcaccg cgatatgctt gatgtcgagc atgccggtgg ttttcggcag
     7381 atgttgcagg ggcgtgttgt cgggatggaa ggccagggga atgaacgttt ggaagccgag
     7441 ggtttcgtct tgcagcgccc gcagcttgag caggtggtcg acgcggtctt catcattttc
     7501 gacgtggccg tagagcatgg tcgcgttcga cttgagcccg atctggtgcg ccaggcgcgc
     7561 ggtttcgagc cactggtcgc catcgatctt gtgatcgcag atgatatggc gcacgcggtc
     7621 agcaaaaatc tcggcgccgc cgccgggcag cgaatccact cctgaggctt tcagttgctc
     7681 cagcgtctcg cgaatcgaaa gcttcgcccg ccgcgacaga taagccactt ccaccatcgt
     7741 gaaggccttg aggtgcacct gcgggaagtg ctgcttcaac ccggaacaca ggtcgaggaa
     7801 atactgaaac ggcagatccg gatg
//
LOCUS       DASPUL010000045         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1651357, whole genome shotgun sequence.
ACCESSION   DASPUL010000045 DASPUL010000000
VERSION     DASPUL010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651357"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..972
                     /locus_tag="VEJ00_01045"
     CDS             361..972
                     /locus_tag="VEJ00_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HXW99771.1"
                     /translation="MVAFEAPAIPSADYAEMARRPLEPQQCALLVIDIQEKLLPPIFQ
                     KEQLVRNAQLLIRAATILKIPALLSTQYAKGLGATVPEIASLLPGTEPIDKTPFSCFG
                     SDAFCGQLKRLPGKRTTLLLCGMESHICVTQTALGALREGYLVHIASDAVSSRTEWNW
                     KIGLQRMRAAGAVISSTEMIIYELMRSSSSAAFKELLPHLRGS"
     gene            1045..2715
                     /locus_tag="VEJ00_01050"
     CDS             1045..2715
                     /locus_tag="VEJ00_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXW99772.1"
                     /translation="MSLRSRFQAVVALAMLGLLVLAAFWLGTERSRILQEKQEKVKNL
                     VEAAHSILGEYYQRQQHGLPQAEAQKQAMELLKTTRYQGDNYFWINDLRPFMVMHPTK
                     PQLNGTDLSGYKDPNGKALFVEMAEAVRRSGAGFVAYQWPRPGTDKPVPKISYVKGFE
                     PWGWIVGTGIYVDDVDALWRQSALKAAALMTLLLVLMAAGSIRSWRRMFGPLDRMVAC
                     MRDVAEGEGDLTRRLDVPPDREVAELARWFNTFMDKLQAILLSVADNIQHLAAAGEQI
                     SATSRQQTQGAAQQKDQTQQVAAATQEMSATVQQVMEISNRTAEAAQKAADAARHGGN
                     VVEESLARMQSIATSTSDAAKKIEYLGEQSKHIGRIVGVIDDIADQTNLLALNAAIEA
                     ARAGTQGRGFAVVADEVRKLAERTTAATKEIAQMIHTVQEGTHLAVAAMQSGTKEVEL
                     GVASTRQAGDSLREIIKISDQVGEMVTHIATAATEQASATDEMSRNTERIAGIAAATA
                     VGAQQTAKATEDLAALATELQGKVGQFRLASESEEERGLRSRAAAGGL"
     gene            complement(2727..3647)
                     /locus_tag="VEJ00_01055"
     CDS             complement(2727..3647)
                     /locus_tag="VEJ00_01055"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW99773.1"
                     /translation="MRERLQRRKKKPAEEKAAAPPPLQPAYFDEEQPEAPAPEASVQE
                     APVPEETQPEAQPARDASRSVEAAEAPRKQAAGGGERRGRSRRRRGGRGRGGGGGREQ
                     AVAQAFAPAAVKALTPELPVAAEAEEAPEREPEAPAPRAPVVPAAVPAAAARPPKGLV
                     VLAIGLPGSGKSSWFKRHNVVPLSSDMVRSLLFDDVREQRFQDLVFSNLRSMLKGRLI
                     AKRPMNYVDATNLTPQERAHWVKLAKDYNYEVHAVFFDVPLEVCIERHQRRDRVVPED
                     VMRRMAAKLKPPSFDEGFAKITVVRVKKKE"
BASE COUNT          730 a         1238 c         1121 g          663 t
ORIGIN      
        1 ccagggattc gaaccccgat atgctgatcc agagtcagct gtcctgccgt tgaacgaggg
       61 ggcagtcttt cgattggcgg gagccggcat gccccggctc ttctcgattc tatgggccgc
      121 ggcgaatgcg gtcaaccggc aggcccatac ccgcggtact tcagtttcaa acctgtcggt
      181 tcctcagcgg tctctgcgca ttctcagccc gctctgcgct ttaaaatctt ctttaaggca
      241 gagatcgcgg agaaatgccg cggagagcgc tgagactggc gcacccgtac acgcgccctt
      301 ttctccagga aattcgctcc gctcacgcta tgatttctga agagatcccc aggagccgtc
      361 atggtcgcct ttgaagcccc cgccattcct tccgccgatt atgccgaaat ggctcgccgc
      421 ccgctcgagc cccaacaatg cgcgctgctg gtcatcgaca tccaggaaaa gctgctgccg
      481 ccaatcttcc agaaagagca gttagtgcgc aatgcgcagc tgctgatccg cgccgccacg
      541 atccttaaga ttcccgctct cctcagcacg cagtacgcga agggcctggg cgcgaccgtg
      601 cccgagatcg cttccctgct ccccggcacc gagcccatcg acaaaacccc gttctcctgc
      661 ttcggcagcg acgcattttg cgggcagctg aagcgcttgc ccggcaagcg caccaccctg
      721 ctgctgtgcg gcatggagag ccacatctgc gtcactcaaa cggcgttggg tgcgctgcgc
      781 gaaggctatc tggtgcacat tgcctccgac gccgtcagct cgcgcacgga gtggaactgg
      841 aagatcggcc tccaacgcat gcgtgccgcc ggagccgtca tctcctccac agaaatgatc
      901 atctacgagt tgatgcggtc gtcgtcgtcg gcggcattca aggagttgtt gccccatttg
      961 cgaggatcgt aaagccgctc gccagacgtc ctcaagcttt tttctgccct gccgatctag
     1021 tctccgggga gtgcaacccc gcgcatgagt ctcagaagcc gatttcaagc cgtcgtcgcc
     1081 ctcgcgatgc tggggctgct ggttcttgcc gccttttggc tgggcaccga gcgatcgcgc
     1141 atcctgcagg aaaagcagga aaaggtaaaa aatctggtcg aggccgcgca ctcgatcctg
     1201 ggcgaatact accagcggca acagcatggc ctgccccagg ctgaggcgca aaaacaagcc
     1261 atggaactgc tgaagaccac gcgctaccag ggcgacaact atttctggat taacgacctt
     1321 cgtcccttca tggttatgca tccgaccaaa ccgcagctga atggcaccga cctttccggg
     1381 tacaaggacc cgaacgggaa agccttgttc gtggaaatgg cggaggcggt gcgccgcagc
     1441 ggcgcggggt tcgttgcgta ccagtggccc agacccggca ccgacaagcc ggtccccaag
     1501 atctcctacg tcaagggatt cgaaccctgg ggctggattg tcggcacagg aatttacgtc
     1561 gacgacgtgg acgcactctg gaggcagagt gcactgaagg ccgcggccct gatgacgctc
     1621 ctgctcgtgc tcatggccgc cggctccatc aggtcctggc gccgcatgtt tggcccgctc
     1681 gaccgcatgg tggcgtgcat gagagacgtc gccgagggcg aaggcgatct tacccgccgc
     1741 ctggatgttc ctcccgaccg cgaggtcgcc gaactggcgc gctggttcaa cactttcatg
     1801 gacaagctgc aggccatcct gctcagtgtc gccgacaaca tccagcatct ggccgcggct
     1861 ggcgagcaaa tctccgccac ctcgcgccag cagacgcagg gagcggccca gcagaaagac
     1921 cagacccagc aagtcgcggc cgctacccag gagatgtctg ccaccgtgca gcaggtcatg
     1981 gaaatctcaa atcgcacggc cgaagccgcc cagaaagccg ctgacgctgc ccgccacggt
     2041 ggcaacgttg tggaggagtc cctggctcgc atgcaatcca tcgccacctc caccagcgac
     2101 gcggccaaga aaatcgagta cctgggcgag caatcgaaac acatcggacg catcgtcgga
     2161 gtcatcgacg acatcgccga ccagaccaac ctgctggccc tgaatgccgc gatcgaggcg
     2221 gcgcgggccg gaacgcaggg ccgcggcttc gccgtcgtag ccgacgaagt ccgcaaactc
     2281 gccgagcgca ctaccgcggc taccaaggaa atcgctcaga tgattcatac ggtccaggaa
     2341 ggcacccatc tggcggtggc ggccatgcaa tccggtacga aggaagtaga actcggcgtc
     2401 gcctccaccc gccaggccgg cgattcgctg cgcgagatta tcaagatctc cgaccaggta
     2461 ggcgagatgg tcacgcacat tgcgaccgcc gccaccgagc aggccagcgc gaccgacgag
     2521 atgagtcgaa ataccgaacg gattgccggt atcgccgccg ctactgcggt cggtgcccag
     2581 caaaccgcca aagccaccga ggatctggct gctttagcga ccgagctcca ggggaaggtg
     2641 ggacagttcc gcctggcgtc ggagagtgag gaggaacggg gtctgcgctc ccgggccgcg
     2701 gccggcggac tctaacgcga gccgttctat tctttcttct tcacccgcac gaccgtgatc
     2761 ttggcaaacc cttcgtcgaa tgacgggggc ttcagcttgg ccgccatgcg ccgcatcacg
     2821 tcctcgggca ccacgcgatc gcggcgctga tgccgctcga tgcagacttc cagcgggacg
     2881 tcaaaaaaaa cggcgtgcac ctcgtagttg taatccttgg caagcttgac ccagtgtgcg
     2941 cgctcctgcg gagtgagatt ggtcgcgtcc acgtagttca tcgggcgctt ggcgatgaga
     3001 cgccctttga gcatggagcg caaattcgaa aacaccagat cctgaaagcg ttgttcgcgg
     3061 acatcgtcaa acaacaacga acgcaccatg tcactcgaca gcggaaccac attgtgacgc
     3121 ttgaaccacg aactcttgcc cgagcccggc aatccgatgg ccagaaccac aaggcccttg
     3181 ggtggccggg cagccgccgc gggtacagcg gcaggaacca ccggtgcgcg gggcgcagga
     3241 gcttccggct cgcgttccgg ggcttcttcc gcttccgcgg caaccggcaa ctcgggagtc
     3301 agggctttta ccgccgcggg agcgaatgcc tgggcgacgg cctgttcccg cccaccaccc
     3361 ccgccgcgtc cacgtccacc ccggcggcgc cgcgaacggc cgcgacgctc tccgcccccg
     3421 gcagcctgct tccggggcgc ttccgcagct tccacgctgc gcgatgcatc acgcgccggc
     3481 tgcgcttctg gttgtgtctc ttcgggcacc ggcgcttcct gaacgctggc ctcaggagct
     3541 ggggcttccg gctgctcctc atcgaaatag gcgggttgca gaggaggcgg cgccgccgct
     3601 ttttcctcgg ccggtttctt cttgcggcgc tgtaagcgct cgcgcatcca tttgcgcatg
     3661 ttcaggcttg taacacagat ggcaggaacg gagcaatcgc agacgaacca ctctgcaaac
     3721 gggaggagcg aagaaacagt tcaagattct tt
//
LOCUS       DASPUL010000046        10122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_692181, whole genome shotgun sequence.
ACCESSION   DASPUL010000046 DASPUL010000000
VERSION     DASPUL010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692181"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VEJ00_01060"
     CDS             <1..628
                     /locus_tag="VEJ00_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXW99774.1"
                     /translation="VCIVIYVLANVAYLRVLSIPEIAASQHVGAAAAEQVLGTRGGAL
                     VSLIILISIIGTLNGCFLTSPRVYFAQARDGLFFQKFAEVHPRYLTPGFAICAQGAWA
                     VVLVVTGSYETLIDYALFALWLSYGLMIAGVIILRRTQPDLPRPYRMWGYPFTAILFL
                     VITAWFLVNTALTKPRPALAGLLLIAAGIPVYFLWKHRSARALDQEEI"
     gene            complement(596..3196)
                     /locus_tag="VEJ00_01065"
     CDS             complement(596..3196)
                     /locus_tag="VEJ00_01065"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXW99775.1"
                     /translation="MALPAGTKLGPYEILAPLGAGGMGEVYRARDARLGREVAIKVLP
                     ESLARDAERLRRFETEARTIAALNHPNILSIHDIGSHEGAPYLVSECLEGQTLRQELG
                     SGALPLRRAVEYGTEIAQGLAAAHEKGIVHRDLKPENVFVTRDGRVKILDFGLAKLTR
                     PETSDQGATVEAVPTSAGVVLGTVGYMAPEQVRGEAADARSDIFALGTILYEMLTGQR
                     AFRRDTSAETMTAILKEDPPELSTSGKLISPGMERVVRRCLEKKPLQRFQSARDLAFN
                     LEGLSGISSTSAAAAVTAPKEPSRRIGLLALGAALLVLVGAGAWLFGRRSVPITSQTY
                     HQLTFDRGLVYAARFAADGRSIYYSAGWNGQPLQIYSTVPDSPESRPLNLINSTVFAI
                     SPSQLAISMGCTDRYVGICMGTLAQVPVSGGSPREVAGDVVAADWTSDGAEMAVIRQA
                     GGKDRVEFPRGKVIYESDHTLGYLRISPRGDALAFLRFTTADGDAGWVIAIDRKGREL
                     LHSNMFVSTEGLAWSPSGEEVWAAAATGDSGWADAIHAFSLGGKERIVLRLPGILRLH
                     DVSRDGRILLSKESWRSGIQFRGPGDSKERDLDWLDYATLRDFSADGKEMAFDDWGAA
                     AGMNGLAYLRKTDGSPAIKLGQWNGPVLSPDGSQVLAVDAHASVLATELRFALLPTGV
                     GEIQKLAPSGVLQTESLGWMPDGRAVYFAGSDGQGWRMYVQDIVGGAARAVTPFISVK
                     RNHWESHLVSADGKLMFARDVNGKGMLYPLAGGEPREIPGWSSEDMWVNWSADGHSAY
                     VYRDEKTSAPVYRLDLATGKREQVATLAPGDPAGVTAVLNVRMTADGKTYGYSFSREL
                     SDLFLVEGAR"
     gene            3334..4587
                     /gene="ispG"
                     /locus_tag="VEJ00_01070"
                     /gene_synonym="gcpE"
     CDS             3334..4587
                     /gene="ispG"
                     /locus_tag="VEJ00_01070"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HXW99776.1"
                     /translation="MATIPRRKTVTAVVGGVRVGSDAPVVVQSMTNTDTADVPSTVQQ
                     VAALARAGSELVRVTVNNDDAAAAVPHIVEQLDKQGVRVPIIGDFHYNGHVLLTKYPE
                     CARDLAKYRINPGNASIGAKGDNNFRTMIEVAVKHNKPVRIGVNWGSLDSVLLTRMMD
                     ENSKLAEPKDAREVTMEAMVVSALNSAELAEKYGLRPDHIILSAKVSGVQDLIDVYRA
                     LAARCNYPLHLGLTEAGMGAKGVVASSAAMGVVLQEGIGDTVRVSLTPAPGGDRTEEV
                     RVAQQILQSMGIRSFTPQVTACPGCGRTTSTFFQEMAEQIQSYLREQMPAWKGRYVGV
                     EEMKLAVMGCVVNGPGESKHANIGISLPGTFEEPKAPVYVDGRLFTTLKGDRIVAEFI
                     EILDEYVDSHYAAKESLREAAPAKV"
     gene            complement(4605..5168)
                     /locus_tag="VEJ00_01075"
     CDS             complement(4605..5168)
                     /locus_tag="VEJ00_01075"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HXW99777.1"
                     /translation="MALIVFLRGVNVGGHRTFRPSVLARELSDYDVVNVGAAGTFVVR
                     KPGSRAEFRAALLRKLPFEAEVALCDGRDLKRLEAENPFGTKPSRPDIVRFVSILARA
                     GRLRARLPVTFPPDGEWLVRVIARNKQFVFGMYRRHMKTIGYLGQIDKLFGMPATTRN
                     WNTILAVLRILKEGKKKGLPHSSQKRA"
     gene            complement(5425..7254)
                     /locus_tag="VEJ00_01080"
     CDS             complement(5425..7254)
                     /locus_tag="VEJ00_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99778.1"
                     /translation="MSFRRVGRLAIVALATLLWASCGQVYRPVVIPVNPVPPNPANFH
                     EVFGVSTNVPANPGTAVQIDVSGDIFIGAANMGVNPNHAAVLPNNSRVFVASAGSVNQ
                     DGTLNLFDADVIMAFTPASVAGGLGPPTTFPLPTAGTGQSSGVVSISEDQAGNVTATL
                     SSPLANAVAGVSVQISGVQVTGYDGSFVISSVNAAQTTIHYSDSTLGLAQSSGGTAMV
                     PLICSYQPDFVATAQNNAVFVANYGVEGGANCNVPNTDSVALLNPASNSVTNIGYLPP
                     GAHPVAMAETPDGQNLYVLNQGLNSISDLSPVDLTPLISSPIPVGSRPEWIAMRPDGR
                     RIYVLTQGDGALGTIDTTTNVQIAGSPQSVGGPGVNFVLYDQSRNRLYVTNPTAGLVF
                     VFDATTDPPTPLPLGTKTGGAITIPAPPACAAQGVTCGPVTPVSVAALIDGSRFYVAS
                     YVTVANTSPCPDPNVTANGCVIPQVTVFDAANFSVGGASVVKTTVFPLLPPIATPVNG
                     VQPYALAPSTFCAPVFPAPVTPARARFRMFAAASVDGSRVYASMCDGGVVAIINTTTS
                     TIATGQNNSEDTLVTDLTAPFSATPVTSGVPPPQSPVFLVTGQ"
     gene            complement(7507..8352)
                     /locus_tag="VEJ00_01085"
     CDS             complement(7507..8352)
                     /locus_tag="VEJ00_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXW99779.1"
                     /translation="MPSSVWSGHLTFGLISMPVRLFSGARSQGISFNMLHRTDKSRLK
                     QQYVCQADGQVVDRADIVKGYEFRKDEYIIIEPDEIKKIEPQTAKTMEILEFVKESEV
                     DPVYFESSYYMMPEEAGRRPYALLTKALEESEYVAIAKITMHNREYTVFLRPTQGGMM
                     LHTMYYADEVKKVEGFGAPEGVELKDAEVKVAHQLIEALAGEWDPEKFKDNFQENLKK
                     LIETKLEGGEVAAVEKPKKLAPVVDLMAALKQSLAQMEGKKKPAATAEAEPERAAAGK
                     SRRGR"
     gene            complement(8539..9915)
                     /locus_tag="VEJ00_01090"
     CDS             complement(8539..9915)
                     /locus_tag="VEJ00_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99780.1"
                     /translation="MSPTSTTTYTLKATNASGSTTATAKVTVTSSGGALTITTTSCPG
                     GTQGAAYAGCTIAATGGSPPYTYSVSTSASISPLPEGMSLNASTGDVASPLIGGQGTY
                     TPEFVVKDAANTQATQSINFAINGNNAFLASVFPSTSIFHHRVDAATTSLPVDTSPAA
                     PIYSGYLSATIKPFFGGSSYLTFPNGIPAIEVPFNQADVAVSTTVYQSYFTSGPIPPY
                     APVEGTGRSTGDRHVLVYLEAGGGNKPALYEMWQGIYENGPWTDSSNALWPDVSGNAL
                     TQQGNGTSDAAGLPVGPLLVNADEVIGVGTPDSPAGTIQHPIRFTVNHMVNYWVWPAT
                     QSAGTGSCSQGGTTIPTESEISQSSPPSSCTMSGPAGEIYRLKASVATPSCAATSPQA
                     NVIITAFRDYGIILADNGSSGGLIGTPDARWNDNDLSCLRSLTLADFEPVNVSSLMVS
                     NDSGATTH"
     gene            9896..>10122
                     /locus_tag="VEJ00_01095"
     CDS             9896..>10122
                     /locus_tag="VEJ00_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99781.1"
                     /translation="MVEVGLTLVEPLAELDVNVPGVMAIVVAPLVAQLSVLLEPETIL
                     AGLAVKELIVGLLAALTVTVMVDLIEPAELVA"
BASE COUNT         1935 a         3245 c         2993 g         1949 t
ORIGIN      
        1 ggtctgcatc gtcatctacg tgctcgccaa cgtcgcctac ttgcgcgtcc tgtcgattcc
       61 ggaaatcgcc gccagccagc acgtcggcgc cgctgccgcc gagcaagtcc tcggcacccg
      121 cggcggcgca ctcgtaagcc tgatcatcct gatctcaatc atcggcacgc tcaacggctg
      181 cttcctcacc agcccccgcg tctacttcgc gcaggcacgc gacggtctct tcttccagaa
      241 atttgccgaa gttcatcccc gttatctaac cccaggcttc gcgatctgcg cccaaggcgc
      301 ctgggcggtg gtcctggtcg tgactggctc ttacgaaact ctgatcgact atgccctctt
      361 tgccctctgg ctctcctacg gcctgatgat cgccggtgtc atcatcctcc gccgcacgca
      421 gcccgacctg ccccgcccct accgcatgtg gggatatccc ttcactgcca tcctgtttct
      481 cgtgatcacc gcatggtttc tggtgaacac ggctttaacc aagccgcgtc cggcgctggc
      541 gggccttttg ttgattgctg ccggaatccc ggtctatttc ctgtggaaac accgctcagc
      601 gcgcgccctc gaccaggaag agatctgaca gctcgcgcga gaaagaatag ccatacgtct
      661 tgccgtcggc ggtcatgcgg acattcagga ccgccgtcac accggcggga tctcccgggg
      721 caagagtcgc aacctgctct cgcttccctg tggcgaggtc caggcggtac accggtgcag
      781 acgtcttctc atctcgatac acgtacgccg agtgcccgtc ggccgaccag ttgacccaca
      841 tatcttcaga cgaccagcca ggaatctcgc ggggttctcc tcccgccagc ggatagagca
      901 tccctttgcc attcacatcc cgggcaaaca tgagtttccc atctgcggac acaagatggg
      961 actcccagtg attccgcttg accgagatga acggcgtaac ggcgcgtgcc gcgccgccga
     1021 cgatgtcctg cacgtacatt cgccaccctt gaccatccga gccggcgaaa tacaccgccc
     1081 taccgtcagg catccaccct agagattccg tttgcagaac cccgctaggt gcaagcttct
     1141 ggatctctcc cacccccgtg ggcaacagcg caaaacggag ctcggtggcc agcaccgacg
     1201 cgtgggcgtc caccgccagc acttgactgc catccggcga gagaacgggt ccattccatt
     1261 ggccaagttt gatcgcgggc gatccgtccg ttttccgcag gtacgcgaga ccgttcatcc
     1321 ccgcggccgc tccccagtcg tcaaatgcca tctctttccc gtccgccgag aaatcgcgca
     1381 gagtcgcgta gtcgagccag tccaggtcgc gttccttgct gtcgcccggc cctcggaact
     1441 gaatcccgct tctccacgac tctttcgaca gcaggatccg cccgtctctc gatacatcgt
     1501 gaaggcgcag gatgccgggc aaacgcagca caatccgttc tttgccgccg agactgaagg
     1561 catgaatcgc atcagcccat cccgaatcac ctgtagctgc cgcggcccac acttcttcgc
     1621 ccgacggcga ccacgccaac ccctccgtgc tgacaaacat gtttgagtga agcaattctc
     1681 tccccttgcg atcaatcgcg atgacccatc ccgcatcgcc gtcggcagta gtaaatcgga
     1741 ggaaggccag ggcgtcgccg cgcggcgaaa tccgcaagta gccgagcgtg tggtcgctct
     1801 cgtagatgac ttttccgcgg ggaaactcca cgcggtcctt ccctcccgct tgacggatca
     1861 cggccatctc cgccccatcc gacgtccagt ccgccgcgac tacatcgccg gcaacttccc
     1921 gcggcgagcc tcccgacacc ggcacttgtg ccagtgtccc catgcagatg ccgacgtagc
     1981 ggtccgtaca acccatcgag atcgccagct gtgacggcga aatcgcgaac accgtggagt
     2041 tgatcaggtt cagcgggcgc gattccgggc tgtccggcac tgtggaatag atctgcagcg
     2101 gctggccgtt ccagcccgcg ctgtagtaaa tcgagcgccc atccgccgca aaacgcgccg
     2161 catagaccag gccgcgatcg aatgtgagct gatggtacgt ctgagaagtg atcggcaccg
     2221 agcggcggcc aaacagccag gcgccggcgc cgaccagtac gagcagcgca gcgcccagcg
     2281 cgagaagccc gattcgcctg ctgggctctt tcggcgcggt aaccgccgca gccgcacttg
     2341 tcgacgaaat gcccgagagc ccttccagat taaacgccag atcccgcgcc gactgaaacc
     2401 gctgcagcgg cttcttttcc aaacatcgcc ggacgacccg ctccatcccg ggagaaatca
     2461 atttccccga agtgctcagc tccggcggat cctctttcag gatcgcggtc atcgtctcgg
     2521 ccgaggtgtc gcgccggaac gcgcgctggc cggtgagcat ttcgtacagg atcgttccca
     2581 gggcaaaaat gtcggagcgt gcatccgccg cctcgccgcg tacctgttcc ggagccatat
     2641 accccacggt ccccagaacc accccggccg aagtcggcac cgcctccaca gtagcgccct
     2701 gatcagaagt ttccggccga gtcagcttcg ccaggccaaa atccagaatc ttcactcgcc
     2761 cgtcgcgcgt gacaaaaacg ttctccggct tcaggtcacg atgaacgatt cccttctcgt
     2821 gcgctgccgc cagcccctgc gcgatctccg tgccatactc caccgcgcgc cgcagcggca
     2881 gtgccccgct gcccagttcc tgtcgcagcg tctgcccttc caggcactcc gagaccaggt
     2941 aaggcgcgcc ctcgtgcgag ccaatgtcgt gaatcgacag aatgttggga tggttcagcg
     3001 ccgcgatcgt gcgggcttca gtctcgaatc gccgcagccg ttccgcgtct cgtgccagcg
     3061 actccggcag aactttgatc gcgacctcgc gccccagccg tgcgtcccgc gcccggtaca
     3121 cttcccccat cccgcccgcc ccgagcggag cgagaatctc atacggacca agcttggtgc
     3181 cggcaggaag tgccatcagt ggccgccatt atagacgcag atctagtcta gtcaacgcgg
     3241 aagccagcct cctgagaatc gcgggtgccc catttctcgc gcgttcgctg cgcgagaagt
     3301 ggggattttc gcgtgctaaa ataagaattc cacatggcca cgatcccacg aagaaagacc
     3361 gtcacggccg ttgtcggggg tgtgcgcgtc ggctcggatg caccagtagt tgtgcaatcc
     3421 atgaccaaca ccgacaccgc cgatgtcccc tccaccgtgc agcaggtcgc cgccctcgcc
     3481 cgcgccggct ccgaactcgt ccgtgtcacc gtcaacaacg acgatgccgc cgctgccgtc
     3541 ccgcacatcg tcgagcagct cgacaagcag ggcgtgcgcg tgcccatcat cggcgacttt
     3601 cattacaacg gacacgttct gctcaccaag tatcccgagt gcgcccgcga tctcgccaag
     3661 taccgcatca atcccgggaa cgcctcgatc ggtgccaagg gcgacaacaa cttccgcacc
     3721 atgatcgaag tcgccgtcaa acacaacaag ccggtgcgca tcggggtgaa ctggggatcg
     3781 ctcgactctg tcctcctcac ccgcatgatg gacgagaatt ccaagcttgc cgagcccaaa
     3841 gacgcccgcg aagtcaccat ggaagcgatg gtcgtcagcg ccctcaactc cgccgagctc
     3901 gccgaaaagt acggcctgcg ccccgaccac atcatcctca gtgcgaaagt tagcggcgtg
     3961 caggatttga tcgacgtcta ccgcgctctc gccgcgcgct gcaattatcc gctgcatctt
     4021 ggcctcaccg aagccggcat gggagccaaa ggcgtggtcg cctcgagcgc agccatgggc
     4081 gtcgtcttac aagaaggcat cggagacacc gtccgcgtct cgctcacgcc cgctcccggc
     4141 ggcgatcgca ccgaagaagt ccgcgtcgcg cagcagattc tgcagtccat gggcattcgc
     4201 agcttcaccc cgcaggtcac cgcctgcccc ggctgcggac gcaccaccag caccttcttc
     4261 caggaaatgg ccgagcagat tcagtcctac ctgcgcgagc agatgcccgc ctggaaaggc
     4321 cgctacgtcg gtgttgaaga aatgaagctc gccgtcatgg gatgcgtggt caacggcccc
     4381 ggcgaatcga aacacgccaa tatcggcatc tcgctcccgg gcacgttcga agagcccaag
     4441 gcccccgtct acgtcgacgg acgcctgttc accacgctca agggagatcg catcgtcgcc
     4501 gaattcatcg aaatcctcga cgaatacgtc gactcgcact acgccgcgaa agaatcttta
     4561 cgcgaagcag cccccgccaa agtgtaagcc gacgggtggc ccactcaagc ccgcttttgg
     4621 cttgagtggg gcagtccctt tttcttcccc tctttcaaaa tccgcaggac cgcgaggatc
     4681 gtgttccagt tgcgggtcgt agccggcatc ccgaatagct tatcgatctg gccaaggtag
     4741 ccgattgtct tcatgtgccg gcggtacatt ccgaacacga attgcttgtt gcgggcgatc
     4801 actcgcacca gccattcccc gtcgggagga aatgtaactg gcagtcgagc gcgcaggcgt
     4861 ccggctctcg ccaggatgct cacgaatcga acaatgtcgg ggcgtgacgg cttggttcca
     4921 aaaggattct ccgcctccag gcgcttaaga tcgcgcccgt cgcaaagcgc aacttccgct
     4981 tcgaatggca gcttccgcag cagcgcagcg cggaactctg ctcgcgaccc gggtttccga
     5041 acgacaaacg tgccggcagc gcctacgttg acgacatcat agtcgctcaa ctctctcgcc
     5101 aggacgctcg gccgaaatgt cctgtggccg ccaacgttga cgccacgcag gaaaacaatc
     5161 agagccatgc ttatcaccaa gtcgccatgg tgcgagcccg gcagctatcg tggtggattc
     5221 cttcgcgaac tttgcggccg ttccttcgcg aactttgcgg tctagctttc aacgcaaaga
     5281 ttgcccgaac ctaaccaatt gcaaacatag gcatttacgt caaagatcat accactgtac
     5341 gtacaatccc gtacataaaa gtgttcctgc aggaacaccg tggaaaacta ccctaaccct
     5401 tggattggca ccagcttaga ggccctactg cccggtcacc aggaacaccg ggctctgcgg
     5461 cggaggcacg ccgctcgtga ctggcgtcgc gctgaacggt gccgtcaggt cggtcaccag
     5521 cgtgtcctcg ctgttgttct ggccggtagc gatggtgctg gtcgtcgtgt tgatgatcgc
     5581 cactacaccg ccatcgcaca tgctcgcgta aacgcggctg ccgtccaccg aagcggcggc
     5641 aaacatgcgg aagcgcgccc tcgccggcgt caccggcgcc ggaaacaccg gcgcgcagaa
     5701 cgtcgaagga gccagtgcgt aaggctgcac cccattgact ggcgtcgcga tcggcggcaa
     5761 gagcgggaag accgtcgtct tcaccaccga cgctccgccc acagaaaaat tcgccgcgtc
     5821 gaaaaccgtc acctgcggaa tcacgcatcc gttcgccgtc acgttcggat cggggcacgg
     5881 agacgtgttc gctacggtga catagctggc gacgtaaaac cggctgccgt cgatgagagc
     5941 cgccacagaa accggcgtca ccggcccgca ggtcacacct tgcgcggcgc acgctggcgg
     6001 tgcagggatc gtgatcgccc cgccggtctt ggtccccagc ggcaatggtg tcggcggatc
     6061 ggtcgtggca tcgaacacga acaccagtcc cgccgtcggg ttggtcacat acagccggtt
     6121 gcggctctgg tcatagagaa cgaagttgac gcccgggccg cctaccgact gaggcgatcc
     6181 cgcgatctgg acgttcgtcg ttgtgtcgat ggtgccgagt gccccgtcgc cttgcgtgag
     6241 cacgtaaatg cgtcgcccgt ccgggcgcat cgcaatccac tccggccggc tgcccaccgg
     6301 gatcggcgaa gaaatcagag gagtcaggtc gacaggcgac agatcgctga tggaattcaa
     6361 tccttggttg agcacgtaca ggttctgccc gtcgggagtt tcggccatcg ccacgggatg
     6421 cgcgcccggg ggcaggtatc caatgttcgt gacgctattg cttgccggat tgagcagcgc
     6481 gaccgagtcg gtgttgggca cgttgcagtt ggcgccgcct tcgaccccat agttcgcgac
     6541 aaagacggcg ttgttctgcg cagtggctac gaaatcgggc tggtaggagc agatgagcgg
     6601 aaccattgcc gtcccgccgg aggactgggc cagtccgagc gtcgaatcgg agtaatgaat
     6661 tgtggtctgt gcggcattga cgctcgagat tacgaagctt ccgtcgtagc cggtgacctg
     6721 cacgcccgag atctggaccg agactcccgc cacggcattc gccagaggac tactaagcgt
     6781 tgccgtgacg ttacccgcct gatcctcaga gatagaaaca acgcctgaag actggcctgt
     6841 gccggccgtc ggcaacggaa acgtggtggg cgggcccagc ccgcccgcga cactggcagg
     6901 agtgaaggcc atgatgacgt cggcatcgaa cagattcaag gtgccgtcct ggttcacgct
     6961 gcccgcgctg gccacgaaaa cgcgcgagtt gttgggaagg acggcggcgt gattcggatt
     7021 cacgcccatg ttggcggctc cgatgaagat gtcgccagag acgtcaatct gcaccgccgt
     7081 gccgggattc gccggcacat tcgtgctgac gccaaaaacc tcgtggaagt tcgcggggtt
     7141 aggaggaacc gggttcaccg ggatcacgac cgggcggtac acctggccgc atgatgccca
     7201 cagcaaagta gccagcgcca cgatcgccag ccggcctaca cgtcgaaaac tcatcggcac
     7261 tccgagaaaa gttcgtttga taagaatcga taattgtaac gattgcccgg catcggcgca
     7321 aaacggcttt cggcttccgc cttccggcgg ccggcttccg gcttccggat ttctggtgat
     7381 gaggagaagg atgcgacgcc atcggactgg ggatgcctgg gttgcttgct cggccgggca
     7441 agggccgagg gaatcagcag aaaactaaat agagacgttc gggaagccga gcgccgggag
     7501 ccgaactcac cttccccgtc gcgactttcc tgccgcagca cgctcgggct cggcttcggc
     7561 cgtggcggcg ggctttttct tcccttccat ctgggcgagg ctctgcttga gcgccgccat
     7621 gaggtcgaca accggtgcca gcttcttcgg cttctcgacg gccgcaactt cgccgccttc
     7681 gagtttggtt tcgatgagct tcttcaggtt ttcctggaag ttgtctttga acttctctgg
     7741 gtcccattcg ccggcgaggg cttcgatcag ttgatgcgcg actttcacct cggcgtcttt
     7801 cagttccacg ccctcgggcg cgccgaagcc ttcgactttt tttacctcgt cggcgtagta
     7861 catggtgtgg agcatcatgc cgccctgcgt ggggcgcagg aatacggtgt attcgcggtt
     7921 gtgcatggtg atcttggcga tggcgacgta ctcgctctct tcgagcgcct tggtgagcag
     7981 cgcatacggt cgccggccgg cttcttccgg catcatgtag tacgacgact cgaagtagac
     8041 gggatcgact tcgctctcct tgacgaattc gaggatctcc atggtcttgg cggtctgggg
     8101 ctcgatcttc ttgatctcgt cgggctcgat gatgatgtat tcgtccttgc ggaactcata
     8161 gcctttgacg atgtcggcgc ggtcgacgac ctgcccgtcg gcctggcaga cgtactgctg
     8221 cttgagccgc gacttgtcgg tgcgatgcag catgttgaag gagatgccct gactgcgcgc
     8281 gccggaaaac aggcgcacgg gcatggagat gagaccaaac gtcaggtgac cggaccagac
     8341 ggaagatggc ataggtgtga cctgccccag aaatggtgca accacttcgc accgagagtt
     8401 tagcgtataa gatgcgattc gcgccgccaa gttggcggca gaatcccaaa ggccaatcac
     8461 cacagagtgg gccgggagga tattagaccc aagggatggt agttgcaaag gcgagtcatt
     8521 cactttccgt cggcctgtct agtgggtggt ggctccgctg tcgttgctga ccatcaggct
     8581 ggagacattc accggctcga agtcggccag ggtcaggctc ctgaggcaag agagatcgtt
     8641 atcgttccag cgggcgtcgg gcgtgccgat gaggcctccg ctcgatccgt tgtcggccag
     8701 gatgatgccg taatcgcgaa atgcggtgat gatgacgttc gcctgcgggc tcgtggcagc
     8761 gcaggacgga gtcgccaccg atgccttcag gcgatagatc tcaccggcag gtccggacat
     8821 agtgcaactt gacgggggag aagactgcga gatttctgat tctgttggaa tggtggtccc
     8881 gccctgcgag caactccccg tacccgctga ttgcgtcgcc ggccacaccc agtaattcac
     8941 catgtggttg accgtgaagc ggatgggatg ctggatggta ccggccggtg aatcgggcgt
     9001 gcccacgccg atgacctcgt cggcgttgac cagcaacggc ccgacaggaa ggccggctgc
     9061 gtccgaggtt ccattgcctt gctgggtcag agcgtttcca gaaacgtcag gccagagtgc
     9121 gttcgaggaa tcggtccagg gaccattctc gtaaattccc tgccacatct cgtagagagc
     9181 gggcttgttt ccgccgccgg cctcgaggta gacgagcacg tgccgatcac ctgtggaacg
     9241 acctgtacct tccaccggcg catacggtgg aattggaccg gaagtgaaat aagactgata
     9301 tacggtagtt gaaaccgcta cgtccgcctg gttgaaagga acttcgatcg caggaattcc
     9361 gttgggaaac gtgaggtagg aactgcctcc aaaaaatggc ttgatggtcg ctgacaagta
     9421 gccgctgtag atcggtgctg cgggagaggt atccacgggc aggctcgtcg tcgcggcgtc
     9481 tacacgatgg tgaaagatgg atgtcgaggg aaagacgctg gccagaaatg cgttgttgcc
     9541 gttgatcgca aagttgatgc tttgcgtggc ctgcgtattg gcggcgtctt tcacgacaaa
     9601 ttcaggagtg tacgtaccct ggccgccaat cagggggctg gcgacatctc cagtggacgc
     9661 attgagcgac atcccttcgg gcaggggaga aatgctggca ctcgtgctta cggagtacgt
     9721 gtaaggcgga gatcctccgg tagcagcaat ggtgcaacct gcataggccg ccccctgcgt
     9781 tccgcccggg cacgaagttg tggtgatcgt caacgcgccg cccgacgatg tcactgtgac
     9841 ttttgccgtc gccgtggtcg aacccgaagc gtttgttgcc ttcaatgtgt aggtggtggt
     9901 tgaggtcggg ctcacgctcg tcgaaccgct cgcagagctc gatgtaaacg ttcccggggt
     9961 gatggcgatc gttgtggcgc cacttgttgc ccagctcagc gtgctgctgg agcccgagac
    10021 aatactggca gggctggccg tgaaggagct gatcgtcggt ttgctggcgg cgcttaccgt
    10081 aacggtgatg gtcgatttga ttgagcctgc ggagttggtg gc
//
LOCUS       DASPUL010000047         5338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_829692, whole genome shotgun sequence.
ACCESSION   DASPUL010000047 DASPUL010000000
VERSION     DASPUL010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829692"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="VEJ00_01100"
     CDS             <1..962
                     /locus_tag="VEJ00_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXW99782.1"
                     /translation="LTYLDGKGLVHSRIKPSNILAVHDQLKLSADQILSATDTNSIRR
                     RDVYDAPETAAGIFSPAGDVWSIGVTLVAVLTQNEDFAREDSQRDPDLPGSIPEPFRG
                     IARECLHLDPKRRCSLAEIKARLQPPARSVPAELEPVPAVPYRSNRAAVTIAMLVAAV
                     VVGLVVFYSRGKNVTAPPAGATNPPATETAQPARQAAQAPPQAPKPEPAPARPVARAT
                     AAGGAVVHQVLPDVSKGARNTITGHVRVSVRVEVDSSGKVTAAKLASPGPSQYFANLA
                     WKAAQRWEFSPPQVDGKPTGSAWLLHFRFGRSGTDASAEHLAR"
     gene            complement(1126..1866)
                     /locus_tag="VEJ00_01105"
     CDS             complement(1126..1866)
                     /locus_tag="VEJ00_01105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmJ/YjtD family RNA methyltransferase"
                     /protein_id="HXW99783.1"
                     /translation="MPALSELDDLVVVLVRPRNPLNIGAAARAMSNFGIHRLRLVNPY
                     AVAFREARSAVGAFELLKKAEGFKTVAEAVADCALVVGTTAVRNRALQHPLRRLNPET
                     GSVIRAQLQSGRVALLFGSEKIGLTNADFSHCHWLLNIPTHEKHVSMNLGQAVAVCVY
                     EIARAEASAPTPHAPSPASAGELEQIVSSLLEALHRSGYVKPGNDAMCEKKVRQLVLR
                     LNLEALDAKVLLGMTRQIVWKLRQPAKP"
     gene            2117..2860
                     /locus_tag="VEJ00_01110"
     CDS             2117..2860
                     /locus_tag="VEJ00_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXW99784.1"
                     /translation="MGNLSTPVSTMKAINPATSVRPAGRILIVEDDPAVQKALRRLFE
                     SEGYTVETQSDGKSALESFQSSAPSAVVLDLRLPKISGRDVCKEIKAQAPACPIVVLS
                     AASDVSDKVLLLELGADDYVTKPFSPRELLARVRAALRHTSRVPEEKLISFDDITADF
                     KKMEVKRGGQPVVLTAQEFKTFQFLVQNADRVISRDELLNEVWGYQNYPSTRTVDNHI
                     LKLRQKLEKDPSSPVHFRTVHGMGYKFVR"
     gene            2887..4761
                     /locus_tag="VEJ00_01115"
     CDS             2887..4761
                     /locus_tag="VEJ00_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXW99785.1"
                     /translation="MKSGGSRPAGEMLPRLRLRTRFLLSMVLVTVGLTTTSLLLVWRS
                     IESNVRQSIAVNLRSSVSAFRDFQHERETLLTSYVALLADLPLTRAIMTGADAPTIQD
                     AAPQIWRDVRSNSDLFALVDRNGKVVALHTKTPGFTQEDAERYFQQALEEDEGSHWWF
                     GAHHLYQAFVEPIYLGSRTEGTLLGFLVIGYEINDRLASEFSKVAGGKVAFSYGNDVV
                     ATTLTPEQVQGESARSLISGSVGDEPQDVRIGDEHFLATSLVLSGSHPTPVRLSVLGS
                     YDQATKFIDPLNRYLVLLGFAAILVGSGLVFFLSHTFTRPLASLVDGVRALEAGDFLH
                     PIDSSGGDEVVELTRAFDRMRASLLKTQRDLLESEQLATIGRMASSISHDLRHSLAAI
                     VANSEFLCDSHLTPAQREELYQEVRTGVNLMTDLIDSLLEFARTRESLSPSYSNASDT
                     IQRAVQAVRLHPRHHARSIDVLCNGPVAGWFDPRKLERVLYNLLLNACEAAPPIGGQI
                     EVTAAEIGGTITISVSDNGSGIPESIRDRLFHPFVSYGKENGTGLGLTVVQKIVQDHG
                     GEVSVERTAEGRTVFRIILPGRPTEAFDNASEPGEHLDALMPAQRDSASQKSISHPAP
                     "
     gene            4819..>5338
                     /locus_tag="VEJ00_01120"
     CDS             4819..>5338
                     /locus_tag="VEJ00_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99786.1"
                     /translation="MKSPGTFAVRAAAFAAATFIMFSLRTQAQEAQVTAASPPGGTTT
                     GAVSELQDQVRQLRLMVEEMRTENAQSRAEMQKLRADLQATRALLEEPGMEQPGRVQP
                     SLDQPETAAVNRQRSNAGDSTPGAPPRSTATPDLAETNLQTAALADRVEKLEDSTALL
                     GAKIDEQYQTKVE"
BASE COUNT         1040 a         1618 c         1665 g         1015 t
ORIGIN      
        1 cacttactta tttggatgga aaagggttgg tgcacagccg catcaagccc tcgaatattc
       61 tggcggtgca cgatcagttg aagctttccg ccgatcagat tctctccgcc acagacacga
      121 actcgatccg gaggcgggac gtgtacgacg cgcccgaaac cgcagccggg attttctcgc
      181 ctgccggaga cgtgtggtcg attggcgtca cgctggtcgc cgtgctgacg cagaatgagg
      241 atttcgcccg cgaggactcg cagcgcgatc cggatctgcc ggggagcatt cccgaacctt
      301 tccgcggcat cgcgcgggaa tgtttgcatc tcgatcccaa acggcgatgt tcgctggcgg
      361 agatcaaggc ccggctgcag cctccggcgc ggtcggtgcc ggcggaactg gaaccggttc
      421 ccgctgtacc ataccgctcg aaccgagcgg ccgtcacgat tgcgatgctg gtggcggcgg
      481 tggtggttgg gctggtcgtt ttttattcgc gcggcaaaaa tgtcacggca ccacccgcgg
      541 gcgccacgaa cccacctgcg acggagaccg ctcagcccgc acggcaagct gctcaggctc
      601 cgccgcaggc ccccaagccg gagcctgcac ccgcgcgtcc ggtcgcaagg gcaacagcgg
      661 ccggtggggc ggtcgtacac caggttctcc ccgacgtttc gaaaggcgct cggaatacga
      721 ttacggggca tgtgcgggtc agcgtgagag tggaagtgga ttcgtcggga aaagtcacgg
      781 cggcgaagct ggcgtcacca ggaccgagcc agtattttgc caacctggcg tggaaagcag
      841 cgcagcgctg ggagttttcg cctccgcaag tggacgggaa gccgacaggc agtgcgtggc
      901 tcttgcattt tcgcttcggg cgcagcggga cagacgcatc agccgagcat ctcgctcgct
      961 agacgtatct gatcgagact cgattccctc aggggctgaa gcccgggttt tgtggcggtt
     1021 ctggacggcg cgcctgaaag gcgcgccctt tcaaagccgg gcagtgagat aagtctattc
     1081 gtttatcagt ttgtcactca tcattcgtct tcgtcctccc tcagttcagg gcttggcggg
     1141 ctgacgcagc ttccagacaa tctgccgggt cattcccagc agaaccttgg cgtccagcgc
     1201 ctcaaggttg aggcgcagca cgagctggcg gacttttttc tcgcacatgg catcattgcc
     1261 cggcttcacg tagccgctgc ggtgcagcgc ttcgagcagc gacgagacga tctgttccag
     1321 ttcccctgcg gaagccggag atggcgcgtg tggcgtcggc gcagaggctt ctgcccgtgc
     1381 gatctcgtag acgcagacag ctactgcctg ccccaggttc atggagacgt gcttttcgtg
     1441 agtggggatg ttgagcagcc agtgacagtg gctgaaatct gcgttggtga gcccgatctt
     1501 ctccgagccg aacaggagcg cgacgcgccc gctctgaagc tgcgcccgga tgacgcttcc
     1561 ggtctccggg ttgagccgtc gcagggggtg ctgcaaggcc cgattgcgca ccgccgtagt
     1621 ccccacaacc agagcgcagt cggcgacggc ttcggcaacc gtcttgaagc cctcggcttt
     1681 cttcagcagc tcgaaggcgc cgaccgcgga tcgtgcctcg cggaacgcga ccgcataggg
     1741 gtttacgagg cgcagacggt gaatgccgaa gttgctcatg gcgcgggctg ccgcgccaat
     1801 attgagggga ttgcgcgggc gcaccagcac caccaccagg tcgtccagtt ctgagagcgc
     1861 gggcaagggc tcaattctac gacagcaaag ccgccgcacc gagtgcgagc gcttcgtttg
     1921 ccgcgaattc acgcagagca agtgcagttc cgtaagctgt cttcacaatg tctggaagtt
     1981 gtaaaaaaaa tgtcaaaaac tgcgccgttt cagggtcttg gccgtcaaac agttgtaagc
     2041 ttcagatgtt gagctacttc cgggggcgta ttccatgggg ccggaagtcc tctctcaaag
     2101 aataggaaca cttgacatgg gcaatctctc cacaccagtc agcactatga aagccataaa
     2161 tcctgccacg tcggtccggc cggcgggccg cattctgata gtagaagacg acccggctgt
     2221 gcagaaggct ctgcggcggc tgtttgaatc agaaggttac acggtagaaa cgcaatcgga
     2281 cggcaagtcg gctctggaga gttttcaatc atcggcaccg tccgccgttg tcctggatct
     2341 gcgtctgccc aagatttccg gacgcgatgt ctgcaaggag atcaaagctc aggccccggc
     2401 gtgtccgatc gtggtgctca gcgccgccag cgacgtttcc gacaaggtgc tgctgcttga
     2461 actgggcgcg gatgactacg tgaccaagcc gttcagtccg cgcgaactgt tggctcgagt
     2521 acgggccgcg ctgcggcaca cctctcgagt tcccgaggaa aaactgatca gcttcgacga
     2581 catcaccgcc gacttcaaaa aaatggaagt caagcgcggt ggccagcccg tggttttgac
     2641 ggcgcaggaa ttcaagacgt tccagttcct ggtgcagaat gccgatcgcg tgatcagccg
     2701 cgacgaattg ctgaacgaag tgtggggcta ccagaactat ccctccacgc gcaccgtgga
     2761 caaccacatc ctcaagctcc ggcaaaaact ggagaaggac ccatcgagcc cggtgcactt
     2821 tcgtacggta cacgggatgg gatataagtt cgtccgttga ctctcgtgaa ttcaccgcag
     2881 gagtccgtga aatccggcgg cagcaggcct gccggagaaa tgctgccgcg tctgcggttg
     2941 cgcactcgtt tcctgctttc catggtgctg gtcaccgtgg gtctgactac gaccagcctg
     3001 ttgctggtgt ggcggagtat tgaaagcaac gtgcggcaaa gcatcgcggt caacctgcga
     3061 agttccgtga gcgctttcag ggacttccag cacgaacgcg agaccttgct gaccagctac
     3121 gtggcgctgc tagcggacct tcctctcacg cgcgccatca tgacgggagc ggacgccccc
     3181 accattcagg acgcagcgcc gcagatctgg cgcgacgtgc gcagcaacag cgatctgttc
     3241 gccctggtcg accgcaatgg caaggttgtg gccctgcaca cgaaaacccc gggattcacg
     3301 caggaagacg cggaacggta cttccagcag gctctcgagg aagatgaggg cagtcactgg
     3361 tggttcggcg cgcaccatct ttaccaggcc ttcgtcgagc ccatttatct cggctcacga
     3421 accgaaggca ctctgctcgg gtttctggtg atcggatacg agatcaacga ccgcctggcc
     3481 tcagaattca gcaaggtcgc cggaggcaag gtggcctttt cctacggcaa cgacgtcgtg
     3541 gctacgacct tgacaccgga gcaggtgcag ggggaaagcg cccggtcctt gatctccggg
     3601 tcggtcggcg acgaaccgca ggatgtacgc atcggcgacg aacacttcct ggcgacctcg
     3661 ctcgtgctgt ccgggtctca tcccacgccg gtgcgactga gcgttctagg atcgtacgat
     3721 caggcgacga agttcatcga cccattgaat cgttacctgg tgctgctggg gtttgctgcg
     3781 atcctggtgg gaagcgggct tgtctttttc ctttcccaca ccttcactcg gccgctggcg
     3841 agcctggttg acggggtgcg cgccctggaa gctggggact tccttcatcc gatcgactcg
     3901 agcggtggcg atgaggtggt agaactcact cgcgccttcg atcgcatgcg cgccagcctg
     3961 ctgaaaacgc agcgcgacct gctggaatca gagcagctcg cgaccatcgg gcgcatggcg
     4021 agttcgatat cgcatgacct gcggcattcg ctggctgcca tcgtggccaa ttcggaattc
     4081 ctgtgtgaca gtcacctgac acccgcgcag cgcgaggaac tctatcagga agtccgaacc
     4141 ggcgtgaatc tgatgacgga cctgatcgat tccctgctgg agtttgcgcg cacgcgcgaa
     4201 tcactcagcc cctcctacag caacgcctcg gacaccatcc agcgggcggt gcaggcagtg
     4261 cggctgcatc cgcggcatca tgcccggtcc attgacgtgc tgtgcaacgg cccggtagcg
     4321 ggctggttcg atccccgcaa actggagcgc gtgctctaca acctgctttt gaacgcctgt
     4381 gaggccgctc cccccatcgg tggccagatc gaagtcactg ccgccgaaat cgggggcacg
     4441 atcaccattt cggtctccga caacgggtcg ggcattcccg aatccattcg cgaccggctc
     4501 tttcatccct ttgtcagcta tgggaaggag aacggaaccg ggctgggatt gacggtagtg
     4561 cagaagatcg tccaggatca cgggggcgag gtatctgtgg agcgtacggc tgagggcaga
     4621 accgttttcc gcatcattct gcccgggcgt ccgacggagg ccttcgacaa cgcgagtgaa
     4681 ccaggcgagc atctagacgc tctaatgccc gcgcagcggg atagtgccag ccagaagtcg
     4741 atttcgcatc cggcccctta ggcacatttt ccaatctctt cgtcacgtgc ccaaccccgg
     4801 atgtggtagg aggacggtgt gaagagccca ggaacctttg cggtccgcgc agccgcattt
     4861 gctgccgcta cgttcatcat gttcagccta cgcacccagg cgcaggaggc gcaggttacc
     4921 gcggcttcgc cgcccggcgg gactaccacc ggcgctgtaa gcgaactcca ggaccaggtg
     4981 cgccaattgc gcctcatggt ggaagagatg cgcacggaaa atgcccagtc gcgtgctgaa
     5041 atgcagaagc tgcgcgcgga tctgcaggcg acgcgggcct tgctggaaga acctggaatg
     5101 gagcagcccg ggcgggtcca acctagcctc gatcagcccg agacagcggc ggttaaccga
     5161 caacgatcga acgcgggcga ttcgactccc ggggcgccac cgcggagtac cgctaccccc
     5221 gatctggctg agacgaatct gcagactgca gcgctcgcag atcgcgtcga gaagctcgag
     5281 gactccaccg cactgcttgg cgccaagatt gacgaacagt accagaccaa ggttgaga
//
LOCUS       DASPUL010000048        12322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1651853, whole genome shotgun sequence.
ACCESSION   DASPUL010000048 DASPUL010000000
VERSION     DASPUL010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 12322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12322)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12322
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651853"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1689)
                     /locus_tag="VEJ00_01125"
     CDS             complement(<1..1689)
                     /locus_tag="VEJ00_01125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXW99787.1"
                     /translation="MGNTVATLETLTLADCKVAQDHAPVDRCEIVTDIVRLEQLSSDW
                     QRLWESDRRAEIFQTPEWSRAWWRAYGESYRLCSIVVFAGQEVAGIVPLVERDGKLQF
                     LGTPEADYADIICEERRAAEVLTLAVQTLLESLPGWKECAFHHLSRHSRIYRYHRELP
                     WTLRAWLRCVPAARQHTIILRNQREEVFKALLGKHHTRRIKNKLQKAGELRFRHLESA
                     QEAAAYIPAFVRQHVRRHAVLGRRSVCSTPEFAEFVRALMREVGLSGRMRFGVLELNG
                     QPLAFALGFEVNGKFMLYQHTFDLDAWHYTPGELLLWSVLDYAKEHISREFDFGKGDE
                     TYKDRFANYSRETFSLYLEPRGPAGMVRSLVRSGQVHLQPVVSRVEKLTKSQPVMLRA
                     FRELRMWATGARSRLQQAHIDGVMVQSAGRLTAELFGKFIWSRRATEVFARETSRWDG
                     DALIGALDTSDRDVSEAQFGDLVDMAWEHSEILTLNELPQCRQRLKNGDRLYVVREKG
                     SVVLLCWVSGWSGAAADSATPRMEATPGTPAVLVDEYWSARARDLSLSYRLLLSI"
     gene            complement(1928..3364)
                     /locus_tag="VEJ00_01130"
     CDS             complement(1928..3364)
                     /locus_tag="VEJ00_01130"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXW99788.1"
                     /translation="MSSIRRRILLDALQLFDTVIMVLAFGAATVALGVGRTITMHDFF
                     SMRVKLVNFLLFFGLLLLWHFLFSGMGLYASHRMSSRASESVDVVKATALASLVLWSA
                     AWVFHIHMASARFLIMFFGLSTGLLVSSRLVLRLVLAKTRVHGRNLRFVLIVGTSPRA
                     IEFAKKIQGNAALGYRVIGFADRTWHGSEQFEKSGYPLACDLDGLPEYLRNNVVDEVV
                     IALPIRSFHDDASRVAALCEQQGIVVRVLSNIFNLQLSQSRAEELAGDSCVMHYRGVM
                     DGWPVVGKRVLDFCIALIALLAVSPVLLLAAALIKLTSPGPVFFKQKRIGQNKRRFTI
                     YKFRSMYVNAEEKLRELEHLNEVSGPVFKIKKDPRITPVGAFLRKTSIDELPQLLNVL
                     KGDMSLVGPRPLPIRDYEGFSEDWHRRRFSVRPGITCLWQVNGRSSIPFEQWMELDMQ
                     YIDKWSLWLDLKILARTIPAVLKGFGAA"
     gene            complement(3552..4322)
                     /locus_tag="VEJ00_01135"
     CDS             complement(3552..4322)
                     /locus_tag="VEJ00_01135"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HXW99789.1"
                     /translation="MSRNFELLHEAGKVQDMLSQPVEMRKLIDESPLPPPAPILPSTP
                     AIQTEGIARDEVMKLVQRLFLSGGQGHRQVVFAGTEAGNGCSWVCARAADLLASQAAG
                     SVCVVDCNMVSPTLHLEYHVENHHGLADALRTDESIRLYTQKLSRSNLWLLSAGSIDK
                     NSEQQLTLEAMRRRLSELRAVFDYVLLDVAPLATADHASTLGKWCDGVALVLKANASS
                     RKGARKVLEGLKTAQIPVLGAVLNQRTFPIPESIYNRL"
     gene            complement(4369..5415)
                     /locus_tag="VEJ00_01140"
     CDS             complement(4369..5415)
                     /locus_tag="VEJ00_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXW99790.1"
                     /translation="MFLDFYGLREEPFGVTPDSRFLYLSPEHREALASLYYGIEAGRG
                     FLALIAVPGMGKTTLLFHLLERFRSTARTAFLFQTQCNSREFMRFLLTELGCESGVGQ
                     DPVLMHDEFNRLLLQEARAGRRCIVIVDEAQNLDFSVLETIRLLSDFETPRAKLLQIV
                     LAGQPGLADKLAHPALAQLRQRISLIGRLNTLPPHEIRNYMQYRLKMAGLKQQTVFTD
                     EAYAMISELSLGIPRNINNLCFNALSLGFALSQKTIGGDIMREVAADLDLSSLVSHPG
                     ERSCPPDPRFDGASRESGDWAQPERPYTQVPPIMASTLGADPRTGETVTPLEAKAYMQ
                     QVVQLLRNGTRNPN"
     gene            complement(5416..7035)
                     /locus_tag="VEJ00_01145"
     CDS             complement(5416..7035)
                     /locus_tag="VEJ00_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99791.1"
                     /translation="MNNFDFDLSSEKANLPITMKDLLDAAFRYKRTALLCFLGILTGA
                     ILAAVLQPAQYSASIKFLVGGERVDPVVSAEPNAPQSAKLPVSEEELNSELELLRGPD
                     VLRQVVVTCGLDKRLGLLDRLLSTTPDRRLAKATARLAKDLQMELVKKSDLIEVRYAA
                     PDPQLAVQVLKVLSDAYIQKHVAVHSPPGQLEFFSQETERYQKDLSDAEAQLKQFSQQ
                     EDGVAPQLNRDITLNKLSEFHASLQQTRAQIAGTEERIKTLEKQAGATPERLTSSTRQ
                     LDDAQVLQPLKATLMNLELKRTELLTKYQPSYPLVEEVDKQISETQTAIAAEEAKPIK
                     EETTDRNPTYAWISEEMAKAKAELSELQARAVATQAIVTTYETKARDLTQKSLVEQDL
                     QRTIKADEENYLLYLHKREQARMSEALDRTRILNVAIAEQPVAPSLPSNSPWPTVFAG
                     LFLGVVVSAGAVGARHFLDPSFRSPAEVSAELKIPVLAAVPVGSSILNSNGNGRNSNH
                     DGNGNYTGLQQDPLVESHDALFSSITKSGRH"
     gene            complement(7050..7775)
                     /locus_tag="VEJ00_01150"
     CDS             complement(7050..7775)
                     /locus_tag="VEJ00_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HXW99792.1"
                     /translation="MSSGLKQIIILTATLAWTCQGGAAQNSAHAVVPVASATAASSNA
                     SNSGGESTFRDRYPRYKVRSGDVFDVSFELSPEFNQTVSVQPDGFITLRGVGDIHVAG
                     QTVPELAATIRAAYGQILHEPLISITLKDFEKPYFIADGQVGRPGKYELRGDTTVTEA
                     VAMAGGFLDSAKHSQVLLFRRIDDQWTSAKIINVKKMQSEGKLQEDLLLHPGDMLFVP
                     KNRLSKLRPILPTASLGTLTKTY"
     gene            complement(7846..8127)
                     /locus_tag="VEJ00_01155"
     CDS             complement(7846..8127)
                     /locus_tag="VEJ00_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXW99793.1"
                     /translation="MTRCLSIEIPFKEQAMDNEQNQTKPADLAGSIFTEAEDQPIASL
                     RSVRAQVECKAILAALKRTGWNRKQAARMLKISYRGLLYKIRQHNITAA"
     gene            complement(8212..8769)
                     /locus_tag="VEJ00_01160"
     CDS             complement(8212..8769)
                     /locus_tag="VEJ00_01160"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HXW99794.1"
                     /translation="MSASGVTMAQYPLVSLPQGQAEMNWFAIRTRSRHEKVAERQLQG
                     QGIDVFYPVVSQIHQWSDRRKVVECPLFAGYAFVRIAPSAEERVRVLRTHGVVELVGG
                     QGYGSPIPAEQIEAVRALVAGNVPFAQRPFLKVGQRLRVRGGALDGVVGILVAQNGSR
                     NLVISVEPIQRSLSIQIDGYQFEAI"
     gene            complement(9124..10527)
                     /locus_tag="VEJ00_01165"
     CDS             complement(9124..10527)
                     /locus_tag="VEJ00_01165"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXW99795.1"
                     /translation="MTRRKALNLRQFQVVIPLMGLGSAAGVLLSLNGCSGVGNHSVSP
                     PPTQHIQHVVVIFQENRSPDNLFQDPVLYQQRGADIAQSGLNSLGQSVTLTGTTLVNV
                     YDLNHSHTAFEQMYDGGKMDGADKIHVSCPKKDPDCAPANPQYMFVNPADVQPYFQLA
                     EQYTFGDRMFQTNQGPSFPAHQFIISGTSAPTATSDLFASENMNAGAINAGCIAAPGT
                     FTRLIDPSGDESQTIYPCFEHPTLTDLLHAKGISWRYYAPSAGSIWTGPDAIQHMCVP
                     QTQNGQLACTGGPWQNVMIPETQVLTDIAKHQLAQVTWVIPNGATSDHAGATDGSGPS
                     WVASIVNAIGNSSYWGNTAIFITWDDWGGWFDHVAPKVINSYEYGFRVPLIVVSPYAK
                     AAHISHVTHDFGSILKFIEETYGLPSLGYADAPADDFVDCFDFNQTPLTFQTINAPLG
                     AEHFLNDTRPPSDPDDD"
     gene            11032..11760
                     /locus_tag="VEJ00_01170"
     CDS             11032..11760
                     /locus_tag="VEJ00_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXW99796.1"
                     /translation="MLAADSTAMGTQLLVDALAREPQFDVIESASNAAAVLQLIKREQ
                     AEVALVSARMGKHPGAGYEVVRHIRAESPATRIIMLLDASERGTVVEAFRAGAHGVFC
                     RTEPFRLLAKCIECVHQGQVWASSTELQFLLEALAQPDLGGLRDSPLSARETDVVRCV
                     TEGLTNREIAHRLKLTEHTVKNYLFRIFDKLGVSSRVEVVLYALGNGEHRNSTLPHAS
                     KPHTDASGRKIVRPAPIAIRKTAT"
     gene            complement(11807..>12322)
                     /locus_tag="VEJ00_01175"
     CDS             complement(11807..>12322)
                     /locus_tag="VEJ00_01175"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXW99797.1"
                     /translation="PATQAPVKEARNEPSARPTRSTPSEPSVPPGGLSIYENGKEIFR
                     MLPNKSANNTAATDRAAGVQTAAALEPEAGTVVELPEAEVEKSLVHRVEPDYPEAALQ
                     QRIQGLVLLDVHIGTDGSVQVVRIAGGPPQLAQASSDAVKQWRFQPHTVKGRAVAVHT
                     TVTLSFRLPQS"
BASE COUNT         2465 a         3748 c         3625 g         2484 t
ORIGIN      
        1 aatcgacaaa agcaaccggt acgacaggga aagatcccga gcgcgcgcgc tccagtactc
       61 atcgaccaga acagccggag ttccgggtgt agcttccata cgcggcgtgg cggaatcggc
      121 tgctgcgcca ctccacccgg acacccagca gagaagcacc acactgccct tctcccggac
      181 gacatagagg cggtcgccgt tcttcagccg ctggcggcat tgcggcagct cgttcagggt
      241 gaggatctca gaatgttccc aggccatatc caccaggtcg ccaaattgcg cctcgctcac
      301 gtcgcggtca ctggtatcga gcgcgccgat cagcgcatcc ccatcccacc gcgatgtctc
      361 gcgggcaaat acttcggtag cccgcctgct ccaaatgaac ttgccaaata gttccgcagt
      421 caggcggcca gcggactgca ccatcactcc atctatgtgg gcctgttgca gacggctccg
      481 cgctccggtc gcccacatgc ggagttcgcg gaatgcccgc aacattacgg gctggctctt
      541 ggtcagtttc tcgaccctgc tgacgaccgg ctgaagatga acctgcccgc ttcgcaccag
      601 gctccgcacc atcccagccg ggccgcgcgg ctcaaggtac agcgagaacg tctcgcgcga
      661 gtagttggca aagcggtcct tgtacgtctc gtcgcccttg ccaaagtcga actcgcggct
      721 gatgtgctcc ttggcgtaat ccagcacact ccacagcaga agctcgcccg gcgtgtaatg
      781 ccaggcgtcc aggtcaaagg tgtgctggta gagcatgaac ttgccgttca cctcaaaacc
      841 gagagcgaaa gccagcggct ggccgttgag ctccagcact ccgaagcgca ttctgccgct
      901 cagccccacc tcgcgcatta acgcacgaac aaattctgca aactccggcg tcgagcaaac
      961 gctacgccgg cccagcaccg catgccgccg cacatgctgg cggacgaaag cgggaatgta
     1021 ggcggccgct tcctgcgcgc tttccagatg gcgaaatcgc aactcgccgg ctttttgcag
     1081 cttgtttttg atgcggcgcg tgtgatgctt ccccagcagc gccttgaaca cctcctcgcg
     1141 ctgattccgc aggatgatgg tgtgctggcg cgccgccgga acacaacgca accacgcccg
     1201 caacgtccag gggagctcgc gatgataacg atagatgcga gaatgccgcg aaagatggtg
     1261 aaaggcgcac tccttccagc cgggaagcga ttccaggagc gtctgcacgg ccagcgtgag
     1321 cacttcagcc gcgcggcgct cttcgcaaat aatgtcggcg tagtcggctt ccggagtgcc
     1381 cagaaactgc agcttgccat cgcgctcgac cagcggcaca atcccggcga cttcttggcc
     1441 ggcgaaaacc acgatcgagc acaggcgata gctctctccg taggcacgcc accaggccct
     1501 ggaccattcc ggagtctgga agatctccgc gcgacgatcg ctttcccaca atcgctgcca
     1561 gtccgacgac agctgctcca ggcgcacgat gtcggtcacg atctcgcaac gatccactgg
     1621 agcgtgatcc tgcgctacct tgcagtcggc aagggttagc gtctccagcg tagctacggt
     1681 attacccata agagaatttc gaactcggag ccgcagtcgt gttcgcggaa aaacgaaccg
     1741 cgacggctca agacacactg tgtgtaaggg agagcacgga aagagaattt caggcgtaac
     1801 gccctcttga actgtcaacg gtgggctgcc aaaagtccgg cgcctcactg taacgggcgg
     1861 tacgtgggga tggtagggtc gagacccaag ttttctgcgc agagtatggc gggtgatggc
     1921 gccgtggtca ggcggcgcca aagcctttca gaactgcagg aatggttctg gccaggatct
     1981 tcagatcgag ccaaagcgac catttgtcga tgtactgcat gtccagctcc atccattgct
     2041 cgaagggaat ggagctccgg ccgttgacct gccacaggca ggtaattccc gggcgcacgc
     2101 tgaagcgccg gcgatgccag tcttcgctga acccttcata gtcccgaatg ggcagcggac
     2161 gcggcccaac caggctcata tcgcccttca gaacattaag cagctgcggc agttcgtcga
     2221 tgctggtctt gcgcaggaag gctcccacgg gtgtgatccg gggatccttc ttgatcttga
     2281 acaccggacc gctgacctcg ttgaggtgct ccagttcccg cagcttctct tctgcattca
     2341 catacatgct gcggaacttg tagatggtaa accgtctctt gttctgtccg attctcttct
     2401 gcttgaagaa gacggggcct ggcgaagtca gcttgatcag cgccgcggcc agcagcaaga
     2461 ccggggatac ggcgaggagc gcgatcaggg cgatgcaaaa atcaaggacg cgtttcccca
     2521 ccaccggcca gccatccatc actccgcggt agtgcatgac gcaggagtcg cccgccagct
     2581 cctccgcccg cgactgcgac agctgcaggt tgaagatgtt ggagagcacc cgcaccacga
     2641 tgccctgctg ttcacagaga gcggcaaccc tggatgcgtc gtcgtggaac gagcgaatgg
     2701 gtaacgcaat caccacttcg tcaacgacgt tgttgcggag gtactcgggc aggccatcca
     2761 gatcacaggc cagcgggtag cccgactttt caaactgctc gctgccgtgc caggtccgat
     2821 ccgcgaagcc gatgacgcga tagcccagcg cggcattgcc ctggatcttc ttggcgaatt
     2881 caatcgcgcg cgggctggtt ccgacaatca gcacgaagcg cagattacgt ccgtgtacgc
     2941 gggttttcgc cagcacaagc cgaagcacca gccggctgct caccagcagt ccggtgctta
     3001 ggccgaagaa catgatgagg aagcgggccg aggccatgtg gatgtggaac acccacgcgg
     3061 ccgaccacaa gacgaggctg gccagtgccg tggctttcac cacatccacc gactcgctgg
     3121 cccggctgga catgcggtgg gaagcataca gtcccattcc agagaacagg aaatgccaga
     3181 gcagcagcag tccgaaaaag agaaggaagt tcaccagctt cacgcgcatg gagaaaaaat
     3241 catgcatcgt gatcgttcgc ccaacgccca gggccacagt ggcggcaccg aaggccagca
     3301 ccatgatcac cgtgtcaaaa agttggagtg catccaacag aatcctgcgt cgaatagaag
     3361 acatagacct tttcccagcg ggggagtcag tctgggcgac catgagggcc ggaaattcca
     3421 aatccggaga gtcgccgcgt ttagttgttt aaagagggat gaagtatggg gaggttttat
     3481 tggttacggt tgctcatcca gcggcgccca cagattatgt atcgggcgag gtgccgggac
     3541 gacatcaaaa atcaaagccg gttgtaaatg ctctccggaa tcgggaatgt tctctggttc
     3601 aagaccgccc cgagaaccgg aatctgggcc gtcttcaacc cttccagaac tttgcgtgcg
     3661 cccttgcggc tggaagcatt ggccttcagg accagagcca cgccatcgca ccatttgccc
     3721 aaagtgctgg cgtgatcggc ggtggccaga ggcgcaacat ccagcaaaac gtagtcgaac
     3781 acggcacgca attccgagag gcgccggcgc atggcttcca gcgtcaactg ctgttccgag
     3841 ttcttgtcga tggagccggc gctcagcagc cacagattgg agcgcgacag cttctgcgtg
     3901 tacagccgga tcgattcatc tgtccgcagg gcgtctgcaa gtccgtggtg attctcgacg
     3961 tgatactcca gatggagcgt gggcgagacc atgttgcagt ccaccacgca aaccgaaccg
     4021 gcggcttgtg aggcgagcaa atcggctgcg cgcgcgcaaa cccaactgca tccgttgccg
     4081 gcttccgtac ccgcaaacac aacctggcga tgtccctgac cgcccgaaag gaaaaggcgc
     4141 tggaccagtt tcatcacctc gtctcgcgcg attccttccg tttgaattgc cggagtgctg
     4201 ggaagaatcg gagccggggg aggcagtggg ctctcgtcaa tgagcttgcg catctccacc
     4261 ggttggctga gcatatcctg cacttttcct gcctcgtgca agagctcgaa atttctgctc
     4321 atgaactaac tccttctcct cagactgggc gattccaccg agagcactct agttcgggtt
     4381 tcgtgtgccg ttccgcaaaa gctgcacgac ctgctgcatg tatgccttgg cttcgagagg
     4441 ggtcacggtc tcaccggttc tgggatcggc tccaagggtg ctggccatga tgggtggaac
     4501 ttgcgtgtat ggccgttccg gctgggccca gtcaccgctc tcccgcgagg caccgtcaaa
     4561 tctcggatcg ggcgggcagc ttctctcgcc cgggtgcgaa accaggctgg agagatccag
     4621 atccgccgcc acttcccgca tgatgtcgcc tccgatggtc ttctgactga gcgcgaaacc
     4681 gagcgacagg gcattgaagc aaagattgtt gatgttgcgc gggattccca ggctcagctc
     4741 cgagatcatc gcgtaagctt catccgtgaa aacggtctgc tgcttcagac ctgccatctt
     4801 cagccggtat tgcatgtagt tccggatctc gtgcggcggc aaggtgttca ggcggccgat
     4861 gagggaaatg cgttgccgaa gttgtgccag cgccggatgc gcgagcttgt cggccaaccc
     4921 cggctgcccg gcgaggacga tctgcagcaa cttggcacgg ggagtctcaa agtcggagag
     4981 caggcgaatg gtttccagca ccgagaaatc caggttctgc gcctcgtcca cgatcacgat
     5041 gcaccgccgc cccgcccgtg cctcctgtaa cagcaggcga ttgaactcgt cgtgcatgag
     5101 cacgggatcc tgccccacgc cgctctcaca cccgagttcc gtaagcagga agcgcatgaa
     5161 ctcgcgtgag ttgcactgcg tctggaacag gaacgccgtg cgggcagtgg agcggaatcg
     5221 ttccagcagg tggaacagca gggtcgtctt ccccatgccg ggaaccgcga tgagggccag
     5281 aaacccgcgc ccggcttcga ttccgtaata cagggaagcg agcgcttcgc ggtgttcagg
     5341 gctgaggtag agaaagcggg agtcgggtgt gactccgaat ggttcttccc ttagaccgta
     5401 gaaatccaga aacatttagt gcctgcctga cttggtgatg gaggaaaaca atgcatcgtg
     5461 ggactcgacg agcggatcct gctgaagacc tgtgtaattc ccattgccgt cgtggttgct
     5521 gttcctgccg ttgccattgc tattgaggat actcgacccg actggcaccg ctgccaggac
     5581 gggaatcttc agctccgcag agacctcagc cggagaccgg aacgacgggt ctaggaaatg
     5641 ccgagccccc accgcaccag ctgacactac tacgccgaga aagagcccgg caaacacggt
     5701 gggccacggc gaattcgagg gcaggctggg cgcgaccggc tgttccgcga tggcgacgtt
     5761 cagaatccgg gtgcggtcca gggcctcgct catgcgcgcc tgttcgcgct tgtgcaggta
     5821 cagaaggtag ttctcttcgt cggcctttat cgtgcgctgc aggtcctgct cgaccagact
     5881 tttctgtgtc aggtcgcggg ccttggtctc ataagtcgtg acgatggcct gcgtggccac
     5941 ggcgcgcgcc tgcagttcgc tcagctctgc cttggccttt gccatttcct cgctgatcca
     6001 cgcgtaggtc gggttgcgat ccgtggtctc ttccttgatg ggcttggcct cctccgccgc
     6061 gatcgcagtc tgcgtctcgc tgatctgctt gtcgacctcc tccacaagag gatagcttgg
     6121 ctgatacttg gtgagcagct ccgtacgctt gagttccagg ttcatcagcg tagctttcag
     6181 cggctgcagc acctgagcgt cgtcgagttg tcgggtcgag gaggtcagcc gctcgggcgt
     6241 cgcccccgcc tgtttttcca gggtcttgat gcgttcttcg gtgccggcaa tctgcgcccg
     6301 cgtctgctgc aggctggcat ggaactcgct cagcttgttc agggtaatgt cgcggttgag
     6361 ctgcggggcg actccgtctt cctgctgcga aaactgtttc agctgggcct cggcgtcgct
     6421 caaatccttc tggtagcgct cggtttcctg cgagaaaaac tccagctgtc ccggcgggct
     6481 atgcacagcg acgtgcttct ggatgtaagc atccgacaac accttcagca cctgcaccgc
     6541 caactgcgga tcgggtgcgg catagcggac ctcgatcagg tcgcttttct tgaccagttc
     6601 catctgaagg tccttggcca ggcgggcggt cgctttggcg aggcgccggt ccggggtcgt
     6661 gctgagcagg cgatcgagca gccccaaacg cttgtcgagg ccacaggtta ccaccacctg
     6721 acgcagaaca tcaggcccgc gtaacagctc aagttcggag ttgagttctt cctcgctcac
     6781 cggcagcttg gccgattgcg gagcgtttgg ttccgcgctg accaccgggt ccacgcgctc
     6841 gcctccaacc aggaacttga tgctggcgct gtattgcgcg ggctggagca ccgccgccag
     6901 aattgcgccc gtcagaatgc caaggaagca aaggagcgcg gtccgcttgt accggaaagc
     6961 cgcgtccaga aggtccttca tggtgatggg aaggtttgcc ttctcactgc tcagatcgaa
     7021 atcaaagttg ttcatcgcgg ctgttccttc tagtaggttt tcgtaagcgt acccagcgat
     7081 gctgtcggca ggatcggtct cagcttggag agcctgttct tgggaacgaa cagcatgtct
     7141 ccggggtgca ggagaagatc ttcctgcagc tttccctccg actgcatttt cttcacgttg
     7201 atgatcttgg ctgacgtcca ctgatcgtcg atgcgccgga acagcagcac ctgggaatgc
     7261 ttggcgctgt ccagaaagcc gcccgccatg gctaccgctt cggtcacagt cgtgtcgcca
     7321 cgcagttcat acttgcccgg acggccaacc tgtccatcgg caatgaaata gggcttttca
     7381 aaatccttga gggtgatgct gatcagcggc tcgtgcagga tttgaccgta ggcggcgcga
     7441 atagtcgcag ccagctcggg tacggtctgg cctgcaacgt ggatgtcgcc caccccgcgc
     7501 agcgtaatga atccgtcggg ctgcacgctg accgtctgat tgaactcggg gctgagctca
     7561 aaggagacgt caaaaacgtc gcccgagcgc accttgtaac gcgggtagcg atcacgaaac
     7621 gtcgattcgc cccccgaatt gctggcgttc gaagatgccg ccgtcgccga cgcgacgggc
     7681 accaccgcat gggcgctgtt ctgagccgcg ccgccctggc aagtccaggc cagcgtcgcg
     7741 gtcagtatga tgatttgctt cagcccgctt gacatctctg gcttctcctc tcctgcacgt
     7801 ccggcgcgaa tcattgcacg ctggaccggt ccaggacacg acggctcagg ccgcggtgat
     7861 attgtgttga cgaatcttgt aaagcaggcc gcgatagctg atcttcagca tgcgcgccgc
     7921 ctgcttgcgg ttccatcccg tacgcttcag cgcggccagg atggctttgc actccacctg
     7981 tgcgcgcacg ctgcgcaatg atgcgattgg ctggtcttcc gcttcggtga aaatcgatcc
     8041 ggccaggtca gccggtttgg tctggttctg ctcgttgtcc atagcctgct ctttgaaagg
     8101 aatttcgatc gacaggcagc gtgtcatgcc atctcttcgg caatcatgca tgcccgacta
     8161 agcccggggg tcgagggaaa ggaaaccagg agaaataaaa atccgcaacg atcaaatcgc
     8221 ttcaaactgg tagccgtcga tctgaatcga caaagagcgc tggatcggct ccaccgagat
     8281 gaccagattc cggctgccgt tctgcgccac caggatccca acaactccgt ccagcgctcc
     8341 gccccgaacg cgaagccgct ggcccacttt caggaaggga cgctgggcaa aaggcacgtt
     8401 accggccacc agcgccctga ccgcttcgat ttgctcggca ggaatcgggc tcccgtaacc
     8461 ctgaccgccc accagttcaa ctacaccatg agtgcgcaaa actcgaaccc gttcttccgc
     8521 cgagggagca atgcggacga aggcgtaccc tgcgaacagc ggacattcca ccaccttgcg
     8581 ccgatcactc cattggtgaa tctggctgac cacgggatag aacacgtcga ttccctgccc
     8641 ttgcagctgc cgctccgcca ctttctcatg gcgggaccga gtccggattg caaaccagtt
     8701 catctccgcc tggccctgag gcaaactcac tagcggatat tgcgccatgg ttacgccact
     8761 tgcactcata gcttcgccct ctgagtcaca attcctgact ctggaaatac tgcgttcaaa
     8821 gacggttctg cgtcgtcagc gacagcctgg caatcactcg gagccagaat ccgtttttct
     8881 ctaagggggg agagctactg ctaagtaacg ccgataaagt cggccagaag agaccgcaaa
     8941 agtgggttca gattatcgac cgtcgcaaat ttgtgcaagt ggtagaaagt aatgctaact
     9001 aaagtctcta ttggccgaaa acccgtgctg gccgggccat ccgaccctat cgaggacagc
     9061 catcggacca cagttcagcc gttggctttg atagtttttc gggcctccga gcagcagcac
     9121 tcctcagtca tcgtccggat cgctcggcgg ccgggtatcg ttgagaaaat gttccgctcc
     9181 caacggcgcg ttgatggttt gaaatgtcaa cggtgtctgg ttgaagtcga agcaatcgac
     9241 gaaatcgtcg gctggagcgt cggcatagcc caggctgggc agcccgtagg tctcctcgat
     9301 gaacttcagg atgctgccaa aatcgtgggt cacgtgcgaa atgtgggccg ctttcgcgta
     9361 cggggaaaca acgatcagcg gcacacgaaa accatactcg taggaattga tcactttcgg
     9421 cgccacatga tcgaaccagc caccccagtc atcccatgtg atgaagatcg ccgtgttgcc
     9481 ccagtacgaa ctgttgccga tcgcattcac gatcgacgcc acccacgagg ggccggaacc
     9541 gtccgtagcg ccagcgtgat cggatgtcgc gccgttcgga atgacccagg tcacctgcgc
     9601 cagttgatgc ttggcgatgt ccgtcaagac ctgcgtttcc gggatcatca cgttctgcca
     9661 aggcccgccc gtgcacgcca gttgcccgtt ttgcgtttgc ggaacgcaca tgtgctggat
     9721 ggcgtcggga ccggtccaga tcgagccggc cgagggagcg tagtagcgcc aactgatgcc
     9781 cttggcatgc aaaaggtcgg tcagggtcgg atgctcaaag cacgggtaaa tagtctgcga
     9841 ttcgtctccc gagggatcaa tcagcctggt aaaagttccc ggcgccgcaa tgcaaccggc
     9901 gttgatggcg ccggcattca tgttctccga cgcgaaaaga tcgctcgtcg ctgtcggcgc
     9961 cgaagtaccg gaaatgatga actggtgcgc gggaaaactc ggtccctggt tggtctggaa
    10021 catccggtcg ccaaacgtgt actgttctgc cagctgaaaa tagggctgca cgtctgcggg
    10081 attgacgaac atgtattgcg gattggcggg cgcgcagtct ggatctttct ttgggcaact
    10141 aacatgaatc ttgtccgctc cgtccatttt tccgccatcg tacatctgct caaacgctgt
    10201 gtgactgtga ttcaggtcgt acacgttcac cagggtggtc ccggtcaacg tcaccgattg
    10261 acccagtgaa ttcagcccgg actgtgcgat gtcggcgccg cgctgctgat aaaggacggg
    10321 atcctgaaac agattgtcgg gactgcgatt ctcctgaaaa atcaccacga cgtgctgaat
    10381 gtgttgggtc ggcggcgggg ataccgaatg attaccgacg ccggagcagc cgttcaggct
    10441 gagcaatact ccagccgcgc tccccaaccc catcagcggg atgacaacct ggaactgcct
    10501 taaattcaaa gccttccgtc gcgtcatgaa agctcacatt ttgctgcttt ggaataaaac
    10561 acgggagact gccactccac tcggcgattg ctcactaaat ctgagccgag gtagctgcca
    10621 tttttggatg gcgcagatga cctacgctgt tgcttttgga agtcatgtgc cccccagcag
    10681 tctcgcgcct ctccgactgt gcgagcttcc gccctgtaag tcgctcagga gtctaagtcg
    10741 cactcagtct tgggaatagc ggtgcctggt aaccatttgc gggttacgga cgtttccggt
    10801 aaccacccgt gagtgctatt tacccggagt agaacttctc ctagcatcga aacagtcctg
    10861 cgctgcacaa gctacaaccg tgcagctcct tcgtggctga ccgcagtccc ccactgagaa
    10921 cggtcagcgg cgagtggcca catcctccgt ttcggcccag gaatagtggc agtcgcttca
    10981 taacaactta tggctcattc agctccgctt gcaggtcagg atcgtctttg tgtgctggct
    11041 gccgacagca cagccatggg aacgcaacta ctggtggacg cgctggcgcg tgaaccgcag
    11101 ttcgatgtga tcgagtccgc ctcaaacgcg gcggccgtac ttcagctgat caagcgcgaa
    11161 caagccgagg tggccctggt cagcgctcgg atgggcaagc acccgggtgc gggctacgaa
    11221 gtggttcgcc acatccgcgc cgaatcgccc gcaacacgga tcatcatgct gctggacgct
    11281 tccgaacgcg gaacggtcgt tgaggctttc cgcgccggag cgcacggcgt gttctgccgc
    11341 accgaaccct tccgcctgct ggccaagtgc atcgagtgcg ttcatcaggg gcaagtctgg
    11401 gccagcagca ccgagttaca gttcctgctc gaagccctgg cccaaccgga tttaggcggg
    11461 ctgcgcgatt ccccgctctc ggcgcgcgag accgatgtcg tgcgctgcgt taccgaggga
    11521 ctcaccaacc gcgaaattgc gcaccgcctg aagctgaccg aacacactgt caagaactac
    11581 ctgttccgca tcttcgacaa actgggcgtc tccagccggg tggaagttgt gctttatgcg
    11641 ctgggaaacg gcgaacatcg caactcgaca ctgccccacg cgtcgaaacc gcacacagat
    11701 gcttccggcc ggaaaatcgt gcggccggcg ccgatcgcga tccgcaaaac cgcgacctag
    11761 ttcgtcttca gtgaaaagcg tggagagttt ctttttgcgc cgggatttat gactgcggca
    11821 ggcggaagct cagggtcacc gtggtgtgca cggctaccgc ccttcccttc acggtatgtg
    11881 gctggaagcg ccattgtttg accgcatcgc tggatgcctg agccagctgt ggcggcccgc
    11941 cggcgatccg cacaacctgc acggaaccat cggttccaat gtgcacgtcc agaagcacca
    12001 gcccctggat cctttgctgc agcgcggcct ccggatagtc tggttctaca cggtgcacca
    12061 gactcttttc cacttccgct tctggcagtt ccacgacagt gcccgcctcg ggctcgagag
    12121 cagcagcggt ctgcactcca gccgcccggt cggtggccgc cgtattgttg gcgcttttgt
    12181 tcggcaacat gcggaagatt tccttgccgt tctcgtagat cgagagcccg cccggaggaa
    12241 cggaaggttc ggatggcgtc gatcgggtcg gccgcgccga aggttcgtta cgagcttcct
    12301 tgactggggc ctgggtggcg gg
//
LOCUS       DASPUL010000049         5651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1378216, whole genome shotgun sequence.
ACCESSION   DASPUL010000049 DASPUL010000000
VERSION     DASPUL010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1378216"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VEJ00_01180"
     CDS             complement(<1..1264)
                     /locus_tag="VEJ00_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HXW99798.1"
                     /translation="MPPRKKSPDISAGPPAKITAAATLPAVSSSSGSKGRIFLMDTMS
                     FIFRAYHAMARQRPMSTKKGLPTAATYVFVNMLRKLRDDFAPEYLAAVFDVAGPTFRD
                     EQAEAVTRIRKFDIKTQTFTEVEYKGYKANRAAMPEDLAQQIPYIRRALEAYRIPILE
                     MERFEADDVIGTLARKAAEASHPVYVVSSDKDMMQLVNERVQILNPPKDNLICDAAKV
                     EEILGVPPERVVDVMALRGDSIDNIPGAPGIGDKGSVEIIKRFGTVEQALEHAAHVEK
                     KTYRESLQNNRETILFSKSMATIDTNVPVELDLESMRVGEPDVDALRGLFTELEFTSL
                     LKELLPVVGVKEAHYSEAKSAADVETVAKAVGVGGALAVAFEPGEAAGVAEEDDEEAV
                     DTAEKPQEAQLSLMSRLDHAVESGSEPIV"
     gene            complement(1293..1796)
                     /locus_tag="VEJ00_01185"
     CDS             complement(1293..1796)
                     /locus_tag="VEJ00_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99799.1"
                     /translation="MSRRRYLAPVLAALLTAGAACNKEAPEPEPTTVPSDHVAPSPVG
                     TSETILQKTFRLKDLNVVPFEIPAHAALPHLHGIFASFVGQAHGESDDTANIDFLILN
                     EEQYSQFAGGHPGDALFSVEGSHNQAVNFDLPPSMSQSVKYYLVFRNPAGSKTNKVVE
                     ASFRVDF"
     gene            complement(1803..2399)
                     /locus_tag="VEJ00_01190"
     CDS             complement(1803..2399)
                     /locus_tag="VEJ00_01190"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HXW99800.1"
                     /translation="MITVAKTVRVETFAEALRQLPEHVFDQTEPVRRFLQETPVDADS
                     LVRYLTWDRQHYTRNLIDRTPFYELIAICWEPGQASSVHNHRDQNCWMAVPMGRLLVE
                     NFHLVHQDLEAGRCQLEPLNTVEMNISHPCAVDPADPVHRVVNPREFNQRAVSLHVYS
                     RPFDTCVVYSPEQGTCGEIELHFNTMFGNAPVGKTVVH"
     gene            complement(2473..4107)
                     /gene="murJ"
                     /locus_tag="VEJ00_01195"
     CDS             complement(2473..4107)
                     /gene="murJ"
                     /locus_tag="VEJ00_01195"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXW99801.1"
                     /translation="MTKLLRFFRPSHQHTAFSATVLLMGTVMLSRVIGYAREAYIAFA
                     FGAGSQTDAYVAAFTLPDFLNYIVAGGAASITFISIYTRFLAQKRDTDAQKTFSIIIT
                     VMTAVMIVGTVLTELFAPQFVRWFVKGFSPAQIELCVHLTRILLPAQIFFYVGGVVSA
                     VLLSHRLFLFPAFGPLIYNLFIILGGVLGGRQLGIASLAYGALAGSFVGPFLASVIGA
                     ARIGTGYRPSFDVMNPAFREWVRLSVPLMLGVSLVTADDWILRHYAAGGIGDIARLNY
                     AKRLFAVPIAVLGQATGQASLPFFARLFNENRMKDFADTVNDSVYRVAAASFLATGWM
                     MAAALPLIDLVYRRGKFLFGDAQTTTIYFFWFSLSLALWSAQGLYARAFYAAGDTLTP
                     MTAVSVVTAASLPVYSILFRTFGVVGLAWASDIGIGANLLVLAWLLHRRKFVSLGELR
                     WGELGKAALTAVVAGAVSFEVAKIVPLSASGRGNRVADLVQLALVTLTWAAAVAAGLW
                     LLRSELPRDLRRRRGTAYPAVAQGGSREILGSGREP"
     gene            complement(4216..5166)
                     /locus_tag="VEJ00_01200"
     CDS             complement(4216..5166)
                     /locus_tag="VEJ00_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99802.1"
                     /translation="MSKHSFVSEAFLGLALCMFAAGNAWSQQGPPTAGGAPAHLLVTV
                     EARHGSSIPVINREDVMVYEGHDRDTVTDWVPAQGEHAALELWILIDDGSSSSLGTQL
                     EDLRQFINGQPDTSKVGVAYMQNGIARVAQNLTSDHGLAAKSLRLPLGMGGADASPYF
                     SLSDLIKRWPASTARREVLMVTDGIDRYYGAGDPEDPYLTEAIDDAARAGVLVSAIYS
                     PGAGHFAHSYWQNYWGQMYLSRLAEETGGEAYYIGFTGAPVSFSPFLDDLSHRLTHQY
                     FLTFLAKPPKKAGWTHVRLSTEVSNADLVSAGKVWVSPGQ"
     gene            5458..>5651
                     /locus_tag="VEJ00_01205"
     CDS             5458..>5651
                     /locus_tag="VEJ00_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99803.1"
                     /translation="MAGTQTMTEFCEQVNPRVLLEMRRKRPFPDMLRVQESWTAAPEK
                     RALLWLAARMPERIGPDHLT"
BASE COUNT         1204 a         1832 c         1591 g         1024 t
ORIGIN      
        1 gaacgatcgg ctcggagcct gattcaacgg catgatccaa ccggctcatg agggaaagtt
       61 gcgcttcctg cggcttctcg gccgtgtcga ccgcctcttc gtcatcctcc tcggccactc
      121 cggcggcttc ccccggctcg aaagccacgg ccagcgcgcc gcccacaccg acggctttcg
      181 ccacggtctc cacatccgcc gccgacttcg cttcgctgta gtgcgcctcc tttaccccga
      241 ctaccggcag cagctccttc agcaacgacg tgaactccag ttcggtgaag agcccgcgca
      301 gggcgtcaac atcgggctcg cccacccgca tggactcaag gtctaactct accggcacgt
      361 tcgtatcgat cgtcgccatg ctcttgctga aaaggatggt ttcgcggttg ttctgcaatg
      421 actcgcgata agtcttcttt tcgacatgag cagcgtgctc cagcgcttgc tctactgtgc
      481 cgaagcgctt gatgatttcc accgacccct tgtcgccgat tcccggcgcg cccggaatgt
      541 tgtcgatcga gtcgccccgc agcgccatca cgtcgaccac gcgctcggga ggcactccca
      601 agatctcttc caccttcgcc gcatcgcaaa tcaggttgtc cttgggcgga ttcagtatct
      661 gcacgcgctc gttgaccagc tgcatcatgt ccttgtcgct ggaaacgaca tacaccggat
      721 gcgacgcctc cgccgccttg cgcgccagcg tcccgatcac gtcatcggct tcgaaccgct
      781 ccatttcgag gatgggaatg cggtaggcct cgagcgcccg gcgaatgtag ggaatctgct
      841 gcgccaggtc ctccggcatg gccgcacgat tggccttgta gcccttgtac tcgacttcgg
      901 taaacgtctg cgtcttgatg tcgaacttcc gaatcctggt caccgcctcg gcctgctcgt
      961 cgcggaaggt cggccccgcc acgtcaaaca ccgccgccag atactcaggg gcaaaatcgt
     1021 cgcgcagctt gcgcagcatg ttgacgaaga cgtaagtcgc agcagtaggg agacctttct
     1081 tggtcgacat cggccgctgc cgcgccatgg cgtgataggc gcggaagatg aacgacatgg
     1141 tgtccatcag aaagatgcgg cccttggaac cgcttgagga cgacaccgcg ggcagcgtcg
     1201 cagccgcagt gatcttggcg ggagggccag cggaaatgtc cggtgatttc ttgcgcgggg
     1261 gcaacccgcc tagtgtagca gcaggatcgc ggctagaagt ccacgcgaaa gctcgcttcg
     1321 accaccttgt tcgtcttact gccagcggga ttgcgaaaga caaggtagta ctttaccgac
     1381 tggctcatcg acggcggaag atcaaaattc accgcctggt tgtgggaacc ttctacggaa
     1441 aacagagcat cgccggggtg accgccggcg aactggctgt actgctcttc attcagaatc
     1501 aggaagtcga tgttcgccgt gtcgtcggac tcaccgtgag cctgcccgac aaatgaggcg
     1561 aatatcccat gcagatgcgg cagcgccgcg tgcgccggga tttcgaaggg aaccacattt
     1621 agatctttca gacggaacgt cttctgcaga atggtctcgc tcgtccccac cggagacggg
     1681 gcaacgtgat ctgacggcac ggtagtgggt tccggctcgg gtgcttcctt attgcaagcg
     1741 gcacccgccg ttagcaaagc cgccagaacc ggggcgagat acctgcgtcg cgacatcacg
     1801 cgtcaatgca ccacagtctt tcccaccggg gcgttgccga acattgtgtt gaagtggagc
     1861 tcaatctcgc cgcaagtgcc ctgctcaggg gaatagacaa cgcaggtgtc gaagggccgg
     1921 gaatacacat gcaggctgac cgcgcgttgg ttgaattcgc gcggattgac cacgcggtgc
     1981 accggatcgg ccggatcgac ggcgcaagga tgagaaatgt tcatctcgac cgtattgagc
     2041 ggttcaagct ggcagcggcc ggcctctagg tcctgatgca cgagatgaaa gttctcgacc
     2101 agcaaccgtc ccatcggaac cgccatccag cagttctggt cgcggtgatt gtggacggaa
     2161 ctcgcctggc caggttccca gcagatcgca atcaactcgt agaatggggt gcggtcgatc
     2221 aggttgcgcg tgtagtgctg ccggtcccag gtcagatacc ggaccaaaga gtccgcatcg
     2281 accggcgtct cctgcaggaa acggcggacc ggttcggtct ggtcgaaaac gtgttccgga
     2341 agctgccgca aggcttccgc aaacgtttcg acacgcaccg tcttggccac ggtaatcatg
     2401 ttgagctcag ggatggagta tagcgcagcg ggataccctg atgtcatcgc cagacgcaaa
     2461 aatctggccg gactagggct cccttcccga tcctaaaatc tcccggctcc ctccctgcgc
     2521 caccgccgga tacgcagttc cccgtcggcg ccgcagatcc cgcggcaact cggaacgcag
     2581 cagccagagc cccgccgcca cggccgccgc ccacgtcagc gttaccagcg cgagctgcac
     2641 caggtccgcc acccggttgc ctcgccctga tgccgaaagc ggaacgatct tcgccacctc
     2701 gaagctgacc gcgcctgcca caaccgccgt caaggccgcc ttgcccagtt cgccccagcg
     2761 cagctcgccc agcgatacga acttccggcg gtgcagcagc caggccagaa ccagcaggtt
     2821 ggctccaatc ccgatatccg aggcccaagc caacccgaca acaccaaacg tacggaacaa
     2881 aatcgagtac accggcagcg aagccgctgt aaccacgctc accgccgtca tcggcgtcag
     2941 ggtgtcgccg gccgcgtaaa atgcccgcgc gtagagcccc tgcgccgacc acagcgccag
     3001 tgagagcgag aaccagaaga aataaatcgt ggtggtctgc gcgtcaccaa agagaaactt
     3061 tccccgccgg tacaccagat cgatcagcgg cagtgctgcg gccatcatcc acccggtcgc
     3121 caggaacgac gcagccgcga cccggtacac cgaatcgttc accgtgtcgg cgaagtcctt
     3181 catccggttc tcattgaaca atcgcgcgaa aaacggcaac gaggcctgtc ccgtcgcctg
     3241 ccctagtacc gcgatgggca cggcaaacag ccgcttggcg tagttgagcc gagcaatgtc
     3301 gccgatcccg cccgccgcgt agtggcgcag aatccagtcg tcggccgtga ccagcgatac
     3361 gcccagcatc agcggcacag acagccgcac ccattcgcga aaagccggat tcatcacgtc
     3421 gaacgaaggc cggtagccag tcccgatccg cgccgcgcca attacgctcg ccaaaaacgg
     3481 accgacaaaa ctccccgcca gtgccccgta ggccagcgac gcaatgccaa gctgccgccc
     3541 gcccagcact cctcccagaa tgatgaacag gttgtagatc agcggcccaa aagccggaaa
     3601 cagaaacagc cggtgcgaca acagaactgc cgaaaccacg cctccgacgt aaaagaatat
     3661 ctgcgccggc agcagaatcc gcgtgaggtg aacgcacaac tcgatttgcg ccggagagaa
     3721 tcccttcacg aaccagcgca caaactgggg cgcgaacaac tcggtcaaga ccgtccccac
     3781 gatcatgacc gcagtcatca cggtgatgat gatggagaac gtcttctgcg catcggtatc
     3841 gcgcttctgg gccagaaacc gcgtgtaaat ggaaataaac gtgatcgaag ccgctcctcc
     3901 ggccacgatg taattgagaa agtcaggcag ggtaaaagca gcgacgtaag cgtccgtctg
     3961 tgatccggct ccgaaggcga aagcgatgta cgcctcgcgc gcatagccga tcacgcgcga
     4021 caacatcacc gtccccatca gcaacacggt cgccgaaaag gccgtgtgct gatgcgaagg
     4081 gcggaagaag cgcagcagtt ttgtcatcga gacccgatca gaagttcaat cttatcggaa
     4141 tctcaaatca cgagcgcgaa actgcgcgcg tccgttcccg aagtggcaat gctgccgacg
     4201 aatcagcggc gatggtcact gccccggcga gacccagacc ttgcctgctg aaaccagatc
     4261 ggcgttcgat acttcggtcg agagccgcac gtgcgtccaa cctgcttttt tcggcggctt
     4321 ggccaaaaaa gtcaggaaat actgatgcgt cagccgatgt gacaaatcat ccagaaacgg
     4381 cgaaaacgag actggcgcgc ctgtgaatcc gatgtaatag gcctcgccgc cggtttcctc
     4441 cgccagtcgt gacaggtaca tctgccccca ataattctgc cagtaactgt gggcaaaatg
     4501 gccagcaccg gggctataaa tcgccgacac cagaacaccg gctcgcgcgg catcgtcgat
     4561 ggcttcggtc aagtaaggat cctccgggtc acctgcgccg taatagcgat cgattccatc
     4621 ggtgaccatc aacacctccc gccgcgccgt gctcgcaggc cagcgtttga tcagatcgct
     4681 taaagaaaaa tacgggctcg cgtcggcgcc gcccatcccc aggggcagac gcagtgactt
     4741 tgctgccagg ccatggtcgc tggtcagatt ctgcgccacc ctggcgatgc cgttctgcat
     4801 ataagccacg ccaaccttcg atgtgtctgg ctgcccgtta atgaactgcc ggagatcttc
     4861 caactgcgtg cccaggctgc tgctggaccc gtcgtcgatc aggatccaga gctccagggc
     4921 cgcatgctcc ccttgcgccg gcacccaatc ggtcactgtg tcccgatcgt gcccttcata
     4981 caccatcaca tcttcgcggt tgatgaccgg aatgctcgaa ccatgacgag cctcaaccgt
     5041 taccagcagg tgcgcgggag ctcctcccgc tgttggcgga ccttgctgcg accaggcatt
     5101 cccggccgca aacatgcaca aggccaaccc aagaaaggct tcggatacaa aactgtgttt
     5161 gctcatcaag acctccttgc aacgacgctt cgccccaggc gccctgaacc acaatacttg
     5221 tcccatcttg ttagatgtac cagcacgtcg aagcgctcag ctttcgacgc ccttctgatc
     5281 acgcacgacc tcctgcgacg ccgaggcaac ccagcaagca attcattttg aagtacttat
     5341 cggttgatct tcgtttgatc ctctcttgag cagccgtgca ttgccgtacc ctgcgttttc
     5401 gccgaatgtt gcagcgaagc ggtgcagtgc atgaggcgga gactctcgga ggcgaacatg
     5461 gcaggaacgc aaacgatgac cgaattctgt gagcaggtga atcctcgcgt gttgctggag
     5521 atgcgccgca agcgcccgtt tcctgacatg ctgcgcgtgc aggaaagctg gaccgctgcg
     5581 ccagaaaagc gtgcgctcct atggctggca gcgcgtatgc cggaacgcat cggcccggat
     5641 cacctgacga t
//
LOCUS       DASPUL010000050         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1515382, whole genome shotgun sequence.
ACCESSION   DASPUL010000050 DASPUL010000000
VERSION     DASPUL010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515382"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VEJ00_01210"
     CDS             <1..784
                     /locus_tag="VEJ00_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HXW99804.1"
                     /translation="VCKDYDLRELFRYINETALFKNQWQLKTASQADYARLVEEKFRP
                     ILRKLEDEVAASGWFEPAVVHGYFPAQSEGNDVIVYQVPSPELQVPSAQRELLRFTFP
                     RQKEGRRLSISDFFAPKSSGGYDVLGLSLVTIGPKATAETQRLFEGGEYTKYLYLHGL
                     SVETAEALAELHHKRMREELGIADEDAPEIRDLFHQKYRGSRYSFGYPACPNLEDQTK
                     LFALLKPEENVGVRLTSGFLLEPEQSTSAIVVHHPAAKYFVV"
     gene            1236..1697
                     /gene="nrdR"
                     /locus_tag="VEJ00_01215"
     CDS             1236..1697
                     /gene="nrdR"
                     /locus_tag="VEJ00_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXW99805.1"
                     /translation="MKCPFCGFANDKVVDSRESKEAESIRRRRECLKCGKRFTTYERI
                     DEIPYMVVKKDGRREKFDRQKVLNGLLRACEKRPVPISKLEQIVNEAESFVIESPERE
                     RKTSELGELIMNRLRRYDKVAYVRFASVYMDFKDVKEFMNELKDLVKTKGA"
     gene            1791..2819
                     /locus_tag="VEJ00_01220"
     CDS             1791..2819
                     /locus_tag="VEJ00_01220"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005962 - mitochondrial isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_component: GO:0045243 - cytosolic isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NAD(+))"
                     /protein_id="HXW99806.1"
                     /translation="MSHKVTLIPGDGIGPEVTQATVRILEATGVKFEWETFAVGSDAY
                     EKYGEYIPKEVLESIERTRVVLKGPVTTPVGGGFASINVALRKKFDLYANFRPIRNLP
                     HIPTRYPDVDLIIVRENTEGLYSGLEHEVVPGVVESLKIMTEKACTRIARFAFEYARK
                     NQRKKIHAIHKANIMKMSDGLFLKCCRSVAKDYSEITYGEHIVDNTCMQLVMNPYQYD
                     MLVMENLYGDILSDLCAAFVGGLGFVPSANLGDDCAMFEAVHGSAPDIAGKNLANPTA
                     LLRSALLMVRHLGEHAASTQIRNALERVYRHREKLTRDIGGQAGTSQFADAVIEEMEK
                     GRAEPARA"
BASE COUNT          671 a          801 c          880 g          597 t
ORIGIN      
        1 tgtatgcaag gactacgatc tgcgcgaact gtttcgctac atcaacgaaa ctgcgctgtt
       61 caagaatcaa tggcagctaa agacggcctc gcaagcggac tacgctcgtc tggtggagga
      121 gaagttccgc ccgatcctgc gcaagctcga agatgaggtg gcagcatccg ggtggttcga
      181 gccggcggtc gtgcacgggt acttcccggc ccagagcgaa gggaatgatg tcatcgtgta
      241 ccaagtccca agtcccgagt tgcaagtccc gagcgcacag cgtgaacttt tgcggttcac
      301 ctttccacgg cagaaggaag gtcgccgtct ctccatctcc gacttcttcg ctcctaagtc
      361 ttctggcggg tacgacgtgc tggggctgtc gctggtcacg atcgggccca aagccacagc
      421 cgaaacgcag cgtctgttcg aaggcggaga gtacacgaaa tacctgtacc tgcacggcct
      481 gagcgtggag acggccgaag cgctcgccga gttgcaccac aagagaatgc gcgaggagct
      541 ggggatagcg gacgaggatg cgcccgaaat tcgcgatctg tttcatcaga agtatcgcgg
      601 gtcgaggtat tcgtttgggt atccagcgtg ccccaacctc gaggatcaaa ccaagctgtt
      661 cgcactcctg aagccggagg agaatgtcgg ggtacggctg acttcggggt tcctgctgga
      721 gcccgagcag agcacttcgg cgatcgtggt gcaccatcct gcggccaagt atttcgtggt
      781 ctgaggcgca gctgaccgtg ggacgtatgc ttaggctgag tcggcgcgct tgccctctct
      841 gaaaatcttt ttacaaaacc cagacaactt tttgacaact caccccaccg ggtcaggcaa
      901 tttatacgca gatcctcatg ctgggcctga cttgcgatag gccccgattc aggggatcga
      961 gagcttcacg tcctccgtca ccgggagggc atggggaaaa acaggcgctc gtaccgcagg
     1021 gggtgcgggc gccgttttgt ttttgtccca gcctgtggga aacggtctcc cgagcagccc
     1081 gcttacctga ggttcctaac ctaactgtta caatctcaag caatcggtca gaaagcagat
     1141 tcccaggtgc ctcccggaca ttgcactccg gacgcggcaa gctggctgcg gtttcgtctg
     1201 gccggcaggc aggaaccgtc gcacgaatca gcgccatgaa atgtcctttt tgcggatttg
     1261 ccaacgacaa ggtagtcgac tctcgcgaga gcaaagaagc ggaatccatc cggcggcggc
     1321 gcgagtgcct caaatgtggc aagcgcttta ccacctacga acgcatcgat gaaattccct
     1381 atatggtggt caaaaaagac ggcaggcggg agaagtttga ccggcaaaag gtactcaacg
     1441 gcctgctccg ggcgtgcgaa aagcggccgg tcccgatcag caaactggag cagatcgtga
     1501 acgaagccga gtcgttcgtc atcgagtcgc cggagcgcga gcgcaagacg agtgagctag
     1561 gcgaactcat catgaaccgg ctgcggcgct acgacaaggt cgcgtacgtg cgcttcgcct
     1621 ccgtgtacat ggatttcaag gatgtgaaag agttcatgaa tgagttgaaa gacctggtga
     1681 agacgaaggg agcataaaca tagtgctcag tgaccagtgg tcagtagcac ccggattctg
     1741 atcactgatc atcagccact gaccacttca attcgcaagc tgagaggctc atgagtcaca
     1801 aagtcacact gatccctggc gacggcatcg gccccgaagt tacgcaggcg actgtgcgca
     1861 tcctggaagc gacgggcgtg aagtttgagt gggagacgtt tgccgtcggc agtgatgctt
     1921 acgaaaagta cggggagtac attccgaaag aggtcctcga gtcgatcgaa cggacgcggg
     1981 tggtgctgaa ggggccggtt acaacgccgg tgggcggagg ctttgccagc atcaacgtgg
     2041 cgctgcgcaa gaagtttgat ctatatgcaa acttcaggcc catccgcaat cttccccata
     2101 ttcccactcg ctatcccgac gtggatctca tcattgtacg ggagaacacc gagggccttt
     2161 attccggact ggagcacgag gttgtgcccg gcgtggtcga gagtctgaag atcatgaccg
     2221 agaaggcctg cacccgcatt gcgcgctttg ccttcgaata cgcacgcaag aatcaacgga
     2281 agaagattca tgccatccac aaggcaaaca tcatgaagat gtcggatggg ctgtttctga
     2341 aatgctgccg cagcgtggcc aaggactatt ccgaaatcac ctatggcgaa cacatcgtgg
     2401 acaacacttg catgcaactt gtgatgaatc cttatcagta cgacatgctg gtgatggaga
     2461 acctgtacgg cgacattctc tccgatctgt gcgcggcgtt cgtgggcggg cttggctttg
     2521 ttcccagcgc gaacctggga gacgactgcg ccatgtttga ggcagtgcat ggctcggcac
     2581 cggacatcgc cgggaagaat ctcgccaacc cgaccgcgct gctgcgctcg gcgctgctca
     2641 tggtgcggca tctcggggag catgccgctt cgacccagat tcgcaacgct ctggagcgtg
     2701 tctatcgcca tcgtgagaag ctgacccgcg acatcggcgg gcaggctgga acttcgcagt
     2761 tcgccgacgc cgtgattgag gagatggaaa aaggcagggc cgagccggcg agagcctaag
     2821 aaatattgct gattgatgat ttttgattgt tgaatgaaaa gctcacgcgg cggggtttca
     2881 ttcaataatc aaaaatcaat aatccacaat agtctgtatc agcgtctctt gagcgcttcc
     2941 aactcctcc
//
LOCUS       DASPUL010000051         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1104095, whole genome shotgun sequence.
ACCESSION   DASPUL010000051 DASPUL010000000
VERSION     DASPUL010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104095"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VEJ00_01225"
     CDS             complement(<1..692)
                     /locus_tag="VEJ00_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXW99807.1"
                     /translation="MPSGVQTEVKTYQMFINGEWVGSKSAKTFPVYDPSTEEVIAQVP
                     DANSDDVNRAVAAAKAAFEDGPWAMTTAQERGRVLFRLADKVRQNLPALAELECRNTG
                     KPIVEAEFDLNDVATCFEYYGGLATKVVGYVNPVPDNAMSLSLKEPVGIAGQIIPWNY
                     PLLMAAWKLAPALAAGCTCVLKPAEQTPLTALEFAKYLEACGVPPGVVNIVTGFGETC
                     GAPIVQHPDVNK"
     gene            complement(744..2207)
                     /locus_tag="VEJ00_01230"
     CDS             complement(744..2207)
                     /locus_tag="VEJ00_01230"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXW99808.1"
                     /translation="MSTIAQPASQPHLIRALGRRDLVLLFVVAVFNLNVVPSIAANGG
                     VTVWLWVISLLLFFWPQGIAVIELAHRYPGEGGVYLWAKEVFGDFHGFLSGWCYWTNN
                     MLYVPTVMLYFVGVSVYVLGPGHQGLADSRVFACITSLTLLAALVYFNILGLGVGKWI
                     NNVGAIGTFIAAAVLIGLGVVIYTRFGTAITAADFKIPADPRFVLNSFGVICFGLVGL
                     ELASVMGDEIQDPRRTLPGAVAWGGVLSGALYVGATLTLLIAVGKSVNVLQGVVQAVT
                     HMAGQVGIDWLSIPFALMLSISIAGIGSAWTGGCARIPFVAGLDSYMPSWLGNIHSRY
                     ATPYAALIVQGIVSAVLVILNFAVAGVQETFQKLLSLAVVLQLIPFVYAFGALVKFAV
                     TESSPKGQYGRATLFLAGLSGLLTTCLGIALVFFPAQQITSLWSYELWMVGGTIFFIG
                     LAAFFFFVYGRRKAPQGRHSLAQDVSPGLAEGVNRVP"
     gene            complement(2284..>3513)
                     /locus_tag="VEJ00_01235"
     CDS             complement(2284..>3513)
                     /locus_tag="VEJ00_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXW99809.1"
                     /translation="SGSKFRDLDGNEYIDHNLTFGALMAGHCHPAVMKAVEKRLSTGT
                     MFGMPHDMEWELAEEICNRFPVEMCRFGNSGTEATMHAVRLARAATGRDKIVKFEGAY
                     HGLHDSALVSVKPHAPDFGDIHAPTSVPGGLGVPKAAVENVKIATFNDLATVERRFKE
                     NPGEIAAIILEPILMNVGLCMPQPGFLKGLREIATKNGALLIFDEVKTGAKLCWGGAS
                     EYFGVTPDMVCLAKSIGGGLPLAAFGTHKSVMDLISQHKVFHGGTYNTNPVSMAAGLA
                     TFRHVLTRENYTHVDKLSKKLADGYRKTIAKVGLQAYIAQAGANGALMLYPKEIVNYR
                     DWTTIDVDLWRHYWFGMVNRGVMAQPYWWDEQWTISVQHTEADIDKHLAAFDEIAASL
                     AKAQQERTERVPAEVAH"
BASE COUNT          748 a         1073 c         1025 g          667 t
ORIGIN      
        1 atcttgttga cgtcgggatg ctgcacaatc ggcgcgccgc aggtctcgcc gaagcccgtc
       61 acgatgttga ctacgcccgg tggaacgccg caggcctcca agtacttcgc gaattcaagg
      121 gcggtcagcg gtgtttgctc cgcgggcttg aggacgcacg tgcaccccgc cgccagcgcc
      181 ggcgccagct tccacgcggc catgagcagc ggatagttcc agggaataat ctgcccggcg
      241 atgccgaccg gttccttcag agaaaggctc atggcgttat cgggaacagg attcacgtag
      301 ccgacaactt tggtagcgag cccgccgtag tattcaaagc acgtggcgac atcgttaaga
      361 tcgaattccg cttccacgat cggcttgccg gtgttgcggc attcgagttc agcgagcgca
      421 ggcaagttct ggcgaacttt gtcggccagg cggaagagga cgcgtccacg ttcctgcgcg
      481 gtggtcatag cccatggtcc gtcttcaaag gcagccttgg ctgccgcgac ggcccgattc
      541 acatcgtcgg aattcgcgtc agggacctgc gcaatgactt cctcagtcga cggatcgtag
      601 acaggaaagg tcttcgccga cttgctgccg acccactcac cgttgatgaa catctggtag
      661 gtcttcactt cggtctgaac tccgctgggc atcttggccc tcctgtaatg cagatcttga
      721 tctccgcgcg agtggtgccg tccctacggg actcggttaa ctccctccgc caacccagga
      781 ctcacgtcct gggctaatga atgccgcccc tgcggggctt tgcgtcgtcc gtacacaaag
      841 aagaaaaatg cagcgaggcc gataaaaaag atcgtgccac caaccatcca cagctcatac
      901 gaccacagcg aggtgatctg ctgcgccgga aagaacacca gcgcgatacc gagacaggtc
      961 gtgagcaatc cgctcaatcc ggccaggaac agcgttgccc tgccgtattg tcctttcggc
     1021 gaagactcgg taaccgcaaa cttcacgagc gccccaaacg catagacaaa agggatcaac
     1081 tgcagcacta cggccaggga aagaagtttt tgaaaggtct cctgaacgcc ggcgacggca
     1141 aaattcagaa tcaccagcac ggcggataca atgccctgca cgatcagcgc cgcgtaaggc
     1201 gtggcgtatc gagaatgaat attgccgagc cacgacggca tgtaggaatc cagtccggcc
     1261 acaaacggga tgcgggcgca accgccggtc caggccgatc cgatgccggc tatggagatg
     1321 ctgagcatca gcgcgaaggg gatcgagagc cagtcgatac caacttggcc ggccatatga
     1381 gtcaccgcct gcacgacgcc ttgcagcaca ttcacgctct tgccgacggc gatcagcagc
     1441 gtaagcgttg ctcccacgta gagcgctcca gacagcaccc ctccccatgc caccgcacct
     1501 ggcagcgtgc gccggggatc ctgaatttcg tcgcccatga ccgaggccag ttccaaaccg
     1561 accaggccga agcaaattac gccgaacgaa ttcagcacga acctggggtc ggcagggatc
     1621 ttgaagtcag ccgcggtgat ggccgttcca aagcgcgtgt aaatcacaac accaagtccg
     1681 atcagtaccg ctgcggcgat gaaggtcccg atcgcgccga cgttgttaat ccatttgcct
     1741 accccgaggc cgaggatatt gaaataaacg agcgcagcca acaaggtgag cgacgtaatg
     1801 caagcaaata ctcggctgtc ggcaaggcct tgatgtccgg ggccaagcac gtacaccgac
     1861 acgcccacga aatacagcat gaccgtcggc acgtagagca tgttgttggt ccagtagcac
     1921 caaccggaga ggaacccgtg gaagtcgccg aaaacttcct tggcccagag atagacgccg
     1981 ccttcaccgg ggtaacgatg cgccaattcg attacggcaa tgccttgcgg ccagaagaat
     2041 aaaagaagag aaataaccca cagccagacg gtaacgccac cgttcgccgc gatcgacgga
     2101 acgacgttca gattgaacac cgcaacgacg aacagcagca cgagatcgcg gcgtccgaga
     2161 gctcggatca ggtgcggctg ggaagcgggt tgtgcgatgg tggacaaggc cgtaatcacg
     2221 ggttagagac tggaggctag ggacgagtgc tggcctctaa cccctagccc ctagtctccg
     2281 gttctagtgc gctacttccg ccgggacgcg ctccgtacgc tcctgctgcg ccttggccag
     2341 cgatgccgct atttcgtcga acgcggccag gtgtttgtcg atgtcggcct cggtgtgctg
     2401 caccgagatc gtccattgct cgtcccacca gtacggctgc gccatcacgc cgcggttgac
     2461 catgccgaac cagtagtgcc gccagagatc cacgtcgatc gtggtccagt cgcggtagtt
     2521 cacgatttcc tttgggtaga gcatcaacgc gccgttggcg ccagcctgcg caatgtatgc
     2581 ctgcaggccg actttggcga tcgtcttgcg ataaccgtcg gccagtttct tgctcagctt
     2641 gtcgacgtgc gtgtaattct cccgcgtcaa cacgtggcgg aaggtggcca gacctgccgc
     2701 catcgaaact ggattcgtgt tgtaggtgcc tccgtgaaat accttgtgct gcgaaatcag
     2761 gtccatcaca gacttgtgcg tgccgaacgc cgccagcggc aggccgccgc caatcgactt
     2821 ggccaggcag accatgtcgg gcgtgacgcc gaagtattca ctcgcgcctc cccagcagag
     2881 cttagcgccg gtcttgactt cgtcaaagat caacagcgcg ccgttcttgg tggcgatctc
     2941 gcgcaggcct ttgaggaagc caggttgcgg catgcagagg ccgacgttca ttaagatcgg
     3001 ttccagaatg atcgcggcaa tctcgccggg attttcctta aacctacgct caacggttgc
     3061 gaggtcgttg aacgtcgcaa tcttcacgtt ctcaaccgct gccttgggga caccgagtcc
     3121 gccggggacc gaagtcggcg cgtggatgtc accaaaatcc ggtgcgtggg gcttcacgct
     3181 gaccagagca ctgtcgtgca agccgtggta cgcgccttcg aatttcacga tcttgtcgcg
     3241 tccggtagcg gcgcgggcca ggcgcaccgc atgcatcgtc gcctccgttc cgctgttgcc
     3301 gaaacggcac atctcgacgg gaaagcggtt gcagatttct tccgcaagtt cccactccat
     3361 gtcgtgcggc atgccgaaca tggttccagt ggaaaggcgc ttttcaacgg ccttcatgac
     3421 tgccggatgg caatgccctg ccatcagcgc tccgaaggtc agattgtggt cgatgtattc
     3481 gttgccgtcg agatcgcgga acttgctgcc gct
//
LOCUS       DASPUL010000052         6492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_144655, whole genome shotgun sequence.
ACCESSION   DASPUL010000052 DASPUL010000000
VERSION     DASPUL010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6492)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6492
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144655"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VEJ00_01240"
     CDS             complement(<1..459)
                     /locus_tag="VEJ00_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99810.1"
                     /translation="MTKRSLLWFLAVFVVSAARAQEPTPPQPVVATVDAGKIGDPISN
                     YDYGMFIEHLGNIINHGLWSEMLDDRKFYFPVNSAEEKEPTGNPMRARFMKLKKWRPV
                     GPDDFVVMDHDHAYVGEQSPAIQLEASTAHGIQQSGLALRKGKSYSGRIVL"
     gene            complement(565..2397)
                     /locus_tag="VEJ00_01245"
     CDS             complement(565..2397)
                     /locus_tag="VEJ00_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99811.1"
                     /translation="MEKKLSRRDLLKSAAGASLALAGIPALADTSSVETPNVTAGGEA
                     APAPAPSRILPLTSTSEVIIPPRGASFMKFSYDFPEPGVEFSGLRLSFRLHTFENTYG
                     LDRGAMTVQEVPGGLDITCTQLVWAGGQQTAPGKLTARIRKNGDQIEWSASAEMERPI
                     KSIVAIVRGVPRGSISAGGLPFRDHKDDEVLLGYPFGAGALFLERGMATMFNTPLAVI
                     QSGGADFFYLSAHCDSVRAHRFYFQPGDSGYRVELVYEQAGWVKSPKLESPLWCAGRA
                     GSMEGACRPHFAHVEKAFQIPDWETRTDVAPWCREAAMAINLHGMHWTGYIFNDYAKM
                     LTILQWVATQIPPRNVFVLLPAWDGRFYWNYPLYKTDPRMGGDEGFRRLIHEGQRLGY
                     HFMPMFGTNCANMLHPEFSQYADATMSQIDGDHFDLNWVDWDNDRHMEGWSPYMNVGV
                     DSWRSWLFHRISYVIDNFGLDAYFLDIVGGWENNTKADSHEGTRRLVADLKEKYPKVM
                     VFGEMHYDALLSFIPVFHVQRESAYWPAYTKYARAFQHLSHPAPGRGSSGLHESGFAP
                     FDPKIVSAESQIIPTVTVVDDTFDRYRDVMADLILQAKQRAKIV"
     gene            complement(2492..4351)
                     /locus_tag="VEJ00_01250"
     CDS             complement(2492..4351)
                     /locus_tag="VEJ00_01250"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HXW99812.1"
                     /translation="MAGVSRRTFLQTATATAAAVYAAHATPSWAAAGRGSVAISPPLA
                     TFAYSEVELLDGPMKRQFEENHARFLNLDDDRLLKVYRQVAGLSAPGDDMGGWYDLTG
                     FSLERNDFHGFIAGHSFGQYLSGLARAYAVTGSKETQAKINRLVKGYAETLDPQAKFF
                     AGYRLPAYTYDKTSCGLIDAYEFAQDPIAMDAHEKLTHAIVQYLPEKALSRQEQRSRA
                     HKDTSFTWDETYTLPENLFLAYQRSGKAFYRDMAKQFLEDDTYFNPLSEGNNVLPFEH
                     AYSHVNAFSSAMQAYITLGSEKHLRAATNGFDMLVKTQSFATGGWGPDETFGEPGRGQ
                     LGDSLAQTHASFETPCGAYGHFKITRYLLRVTKDAKYGDSMERVLYNTILGAWPIQAD
                     GTSFYYSDYATTGKKVWYRDKWPCCSGTFPQLAADYHISTYLRSADGVYVNLFTPSRV
                     QWSDASGRYGLKQETQYPFDNKIQIQVSGSQAKDYAIYLRIPSWATSDSVLSVNGKRV
                     SDPVQPGSFAAIRRTWKDGDRIELELPMPLRLETVDANHPNLVALVRGPLVLFAVADS
                     QPAFDRNALLQAKAANNSKGDWLATAADGSSVSMRPFMNIDKESYSTYLLLKA"
     gene            4698..6317
                     /locus_tag="VEJ00_01255"
     CDS             4698..6317
                     /locus_tag="VEJ00_01255"
                     /inference="COORDINATES: protein motif:HMM:NF018647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HXW99813.1"
                     /translation="MSSISRRSFLRQAGAGVVCCANFAAKHSLLAQSVPASQSARVYV
                     DFRRTIAALDRNLFGSFLEHLGRAIYEGIYDPGSKISDGNGFRKDVLNEIHQLGVPII
                     RYPGGNFVSGYNWLDGVGPKEKRPRVLDKAWNTIESNQFGTNEFLAWCKAVGTQPLMG
                     LNLGTGTPEEAAALVEYCNVEKGTRWSDLRRQHGVADPWKVQHWCLGNEMDGPWQIGH
                     MSATEYGQKAQDAARQMRDVDPSLELIACGSSGPGMRTYLEWDREVLEQCYDYVDGLS
                     LHRYFGNWERDSGGDTAKYLALNLSMEKQIAETVAVCDLVRGHKRSPKKLWLSFDEWN
                     VWYRTTGGDDVDGHRKEAPHLLEEVYNLEDALLVGGLINSLIRNADRVKLACLAQLVN
                     VIAPIMTNADGLLRQTIYYPYSWALQFAKGTVLELLVESSTYEVSGMDPVAHVDVAGS
                     LDRSAGQLSLFLLNRDLSKSHDVEVLWEDAAPARVISASVLTGDDLKAVNSFSAPQRV
                     VPQPFPVPASSGGRTKFELPARSYTVVRWAL"
BASE COUNT         1270 a         1830 c         2038 g         1354 t
ORIGIN      
        1 cagcacgatc ctgccgctgt atgacttgcc cttgcgcagg gcgaggccgg actgttgaat
       61 gccgtgggcg gtgctggcct ccagctggat tgccgggctc tgctcgccaa cataagcatg
      121 gtcgtggtcc atcacaacga aatcgtccgg cccgacgggg cgccactttt tcagcttcat
      181 gaatcgcgcg cgcatcgggt ttccggttgg ctccttttct tccgccgaat tcaccggaaa
      241 gtagaacttg cggtcgtcga gcatctcgct ccacaacccg tggttgatga tgttgcctag
      301 gtgctcgatg aacatgccgt agtcgtagtt cgagatcggg tcgccgattt tgccggcgtc
      361 gacggtagca acaacgggtt gcgggggagt cggctcttgc gcgcgtgctg ctgaaacgac
      421 gaacactgca agaaaccaca gcagacttcg cttggtcata agagacgctc ctttcggcgg
      481 cttacgattt gacgctagat atctttcgac tgcgccgcct tgcgctctgc gcaaggcaac
      541 ttcgctcagg atgacacaca cacatcagac gatcttcgcc cgctgtttcg cctgcaggat
      601 gagatcggcc atcacgtcgc gataacggtc aaacgtgtcg tcgacgaccg taaccgtcgg
      661 gatgatctga ctctcagccg aaacgatctt cgggtcgaag ggcgcaaagc cggactcgtg
      721 caagccgctg cttccgcggc cgggcgcggg atggctcagg tgctggaagg cgcgggcata
      781 cttggtatac gcgggccaat atgccgactc gcgctgcacg tgaaacaccg ggatgaacga
      841 aagcagcgcg tcgtagtgca tctcgccgaa gaccatcacc ttgggatatt tctctttcaa
      901 atcggcgacc aggcggcgcg tgccttcgtg ggaatcggcc ttggtgttgt tctcccagcc
      961 gccgacgatg tcgaggaagt aggcgtcgag accgaagttg tcgatcacgt aggaaatgcg
     1021 atggaagagc cagctgcgcc acgagtcaac gccaacgttc atgtaagggc tccagccctc
     1081 catgtggcgg tcgttgtccc aatcgaccca gttcaggtcg aagtgatcgc cgtcgatctg
     1141 cgacatggtc gcgtcggcgt actgcgagaa ctccggatgc agcatgttcg cgcaattggt
     1201 gccgaacatg ggcatgaagt ggtagccgag cctttggcct tcgtgaatca ggcggcggaa
     1261 tccttcgtcg ccgcccatgc gcggatcggt cttgtagagc gggtagttcc agtagaagcg
     1321 gccatcccac gcgggcagca gcacgaagac atttcggggc ggaatctggg tcgcgaccca
     1381 ctgcagaatc gtaagcatct tggcatagtc attgaagatg tagccggtcc agtgcatgcc
     1441 atgaagattg attgccatcg ctgcttcgcg gcaccaggga gcgacgtcgg ttcgcgtctc
     1501 ccagtcggga atctggaaag ccttttccac gtgggcgaaa tgcgggcgac aagcgccctc
     1561 catgctgccg gcgcggcccg cgcaccagag cggactctcc agctttgggc tcttcaccca
     1621 gcctgcctgc tcgtacacca gttcgacgcg atagccgctg tcgcccggct gaaagtaaaa
     1681 gcgatgggcg cggacgctgt cacaatgcgc agaaagatag aagaaatcag cgccgccgct
     1741 ctgaatgacg gcgagcggcg tgttgaacat ggtcgccatg ccgcgttcga ggaacagcgc
     1801 gcccgcgccg aagggatagc caagcaggac ttcatcgtct ttgtgatctc tgaaaggcag
     1861 tcctccggcg gagatgctgc cgcgtggaac gccgcgaacg attgcgacga ttgatttgat
     1921 gggcctctcc atctcggctg aggcgctcca ctcgatctga tcgccgttct tgcgaatgcg
     1981 tgccgtcagc tttcccggag cagtctgctg tccgccggcc caaaccagct gcgtgcaagt
     2041 aatatcaagg ccgccgggga cctcctgcac cgtcattgcc ccgcgatcca ggccataggt
     2101 gttttcgaac gtatgcaagc gaaaggagag gcggagcccg gaaaattcga cccccggctc
     2161 ggggaagtcg tagctgaact tcatgaagct ggcgccgcgc ggcggaatga ttacctcgct
     2221 ggtagaagta aggggaagga tccggctggg agcgggtgca ggtgccgctt cgcctccggc
     2281 ggtcacgttc ggcgtttcga cacttgatgt gtcggcaagc gcgggtattc cggcaagcgc
     2341 gagcgacgcg ccagccgcac ttttcagcag gtcacggcgg gagagcttct tttccatgat
     2401 cttgagcctc tttgaggaaa cggttcccac ggcccacagc caagccgggg tttgaaacag
     2461 aggggtcact tcaacaacga aaaatcactg ctcacgcctt caacaacaaa tacgtgctgt
     2521 agctttcctt gtcgatgttc atgaacggac gcatcgatac gctgctgccg tcggccgcgg
     2581 tcgcgagcca gtcgcctttc gagttgtttg ccgctttcgc ctgcaacaga gcgtttcggt
     2641 cgaacgcggg ctgggaatca gctacggcaa agagcacgag cggaccccgc accaacgcca
     2701 ccaggttggg gtgattggcg tccacggttt caaggcgcag cggcatcgga agttcgagtt
     2761 cgatgcgatc gccatctttc caggtacgac gaatggctgc gaaactcccg ggttgaacgg
     2821 gatcagacac tcgttttccg ttgacggaga gtacggaatc ggacgtagcc cagctgggga
     2881 tgcgcagata gatcgcatag tccttggcct gagagccgga cacctgtatc tggatcttgt
     2941 tgtcaaacgg atattgcgtt tcctgcttca gtccgtagcg tccgctggca tcgctccact
     3001 gcacgcgcga aggcgtgaag agattcacat agacgccatc tgccgatcgc agatacgtgc
     3061 tgatgtggta gtcggcggca agctgcggga aagttccgga gcagcacggc cacttgtctc
     3121 tgtaccagac tttcttgccg gtggtggcgt aatcggagta gtagaacgat gtgccgtcgg
     3181 cctgaatcgg ccaggcgccg agaattgtgt tgtagaggac gcgctccata ctgtcgccgt
     3241 atttggcgtc tttagtgacg cgcaacaggt agcgagtgat cttgaaatgt ccgtaagcgc
     3301 cgcacggcgt ttcaaagctg gcgtgggttt gcgcgaggct gtcgcccagc tggccgcggc
     3361 cgggttcgcc gaaggtttca tccggacccc agccgcccgt cgcgaaactc tgggtcttga
     3421 ccagcatgtc gaatccattc gtggccgcac gcaggtgctt ctcgctaccc agcgtgatgt
     3481 aggcctgcat cgcagaactg aacgcattga catggctgta ggcgtgttcg aacgggagca
     3541 cgttgttgcc ttcagaaagc gggttgaaat aggtgtcgtc ctctagaaac tgcttggcca
     3601 tgtcgcgata gaaagccttg ccgctgcgct gataggcgag gaacagattc tctggcagcg
     3661 tgtaggtttc gtcccaggta aacgacgtgt ctttgtgggc gcgggagcgt tgctcctggc
     3721 gtgaaagggc tttttccggc agatactgca cgatggcatg ggtcagcttt tcgtgagcgt
     3781 ccatcgctat ggggtcctgc gcgaactcgt aagcgtcgat caggccgcag gaagtcttgt
     3841 catacgtgta cgccggaagg cgatagccgg cgaagaattt tgcctgggga tcaagcgttt
     3901 ccgcatatcc cttgaccagc cgattgatct tggcctgcgt ctctttcgat cccgtaaccg
     3961 cgtacgcgcg ggccaggccg gacaaatatt gtccgaagct gtggccggcg atgaagccgt
     4021 ggaaatcgtt gcgctccagg ctgaacccgg tcaggtcgta ccaccctccc atgtcgtcgc
     4081 ccggcgcgga caagccggcc acctggcgat agaccttgag cagacgatca tcgtcgagat
     4141 tgaggaagcg ggcatgattt tcttcaaatt gccgtttcat gggaccgtca agcagctcta
     4201 cctctgagta ggcgaatgtc gcgaggggag gactgatggc gacgctgccg cggccggcgg
     4261 cggcccagga gggtgtggca tgtgcggcat aaactgcggc ggcggttgcg gtagcggtct
     4321 ggagaaacgt tcggcggctg actccggcca tgggggagcc tcctgaaagg tgggtagcaa
     4381 acgtttacta cggctagctt cctccgcccg tcccactttg tcaactgctt cctccgagca
     4441 gcctgtgaac ttctcgagaa accgttggtc cgcgtgtggg cggggtgcga aaaactgctt
     4501 gacactcgtt gatcagcgaa ctataagctg gtctggctca tgggagcaat cgtttacttt
     4561 gtccacgagc gacgtttcgg gcgcccattc tgttcaacac caactgcgga atttcagtac
     4621 tcttcggcaa ttgcggcaca ccgctgcaca cgcgcgggga ttgaccttca acaagccact
     4681 ttggggaaac aacgtctatg tcttcaattt cacgccgaag tttccttcgt caggcgggtg
     4741 ctggtgtggt gtgctgcgct aacttcgccg ccaagcattc tctgctggcg cagtctgtgc
     4801 ccgcgtcaca gtccgcgcgc gtttacgttg atttccgccg caccatcgct gcgctggatc
     4861 gcaatctctt cggctctttt cttgagcacc tcggacgcgc catctacgag ggcatctacg
     4921 acccgggatc gaaaatctcc gatggcaacg gattccgcaa ggacgtcctc aacgagattc
     4981 accagctcgg agttcccatc attcgctatc ccggcggcaa ttttgtctcg ggctacaact
     5041 ggctcgacgg cgttggtcca aaagaaaaac ggccgcgggt tctcgacaag gcgtggaaca
     5101 cgatcgaatc gaaccagttc gggaccaacg agttcctggc ctggtgcaag gcggtcggca
     5161 cccagccgct gatgggtctg aatcttggaa ccggcacacc ggaagaagca gcggcgctgg
     5221 tggagtattg caacgtggag aagggaacgc gctggagcga cctgcgacgc cagcatggag
     5281 tggccgatcc gtggaaagtc cagcattggt gtttgggcaa cgaaatggac ggaccctggc
     5341 aaatcggcca tatgtcagcg accgaatatg gccagaaggc gcaggacgcc gcgcggcaga
     5401 tgcgcgacgt cgatccgtcc ctcgagctga tcgcctgcgg atcgagcggc cccggcatgc
     5461 gcacgtatct ggagtgggac cgcgaggtgc tcgagcagtg ctacgactat gtcgacggcc
     5521 tttcgctgca ccggtatttc ggcaactggg agcgcgacag cggcggcgat acggccaagt
     5581 atctggctct caatctcagc atggagaagc aaattgcaga gacggttgct gtttgcgatc
     5641 tcgtgcgcgg gcacaaacgg tcgccgaaaa agctgtggct gtccttcgac gagtggaatg
     5701 tgtggtatcg cacgacgggc ggcgacgacg tcgacggcca ccggaaggaa gcgccgcact
     5761 tgctggagga ggtctacaac ctcgaggatg cgttgctggt cggagggttg atcaattctc
     5821 tgattcgcaa tgctgatcga gtgaagctgg cttgcttggc gcagttggtc aacgtcattg
     5881 cgcccatcat gacgaacgca gacggactcc ttcgccagac aatctactat ccctatagct
     5941 gggcgcttca atttgcgaaa gggacggtgc tcgagttgct ggtcgagtcc tcaacttatg
     6001 aagtgtccgg tatggatccc gtggcgcatg tcgatgtcgc cggatcgctc gatcgtagtg
     6061 cagggcagct ctcgctgttt cttctgaacc gtgatctgtc gaaatcccac gacgtcgaag
     6121 tgctgtggga agatgccgcg ccagcgcgtg tgatctcggc gtcggtgctg acaggagatg
     6181 atttgaaggc agtaaacagt ttttctgctc cgcaacgagt ggtaccgcag ccgtttccgg
     6241 tgcccgccag cagtggcggt cgaacgaagt ttgagctgcc ggcgcgttct tacaccgttg
     6301 tgcggtgggc actatagaag ggcgttgtag aagggaagac acatgaggaa aaagggaagc
     6361 atcacccgaa gggagttcgt cgccgcggcg gtctcgaccg cggctgtgtc gtctctggcc
     6421 cacccgcgct gcgtcgctga agtgattggc ggcaaggcgg gcgtggccca ggcgtcggtt
     6481 ccgcttacgg ac
//
LOCUS       DASPUL010000053         7775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_830563, whole genome shotgun sequence.
ACCESSION   DASPUL010000053 DASPUL010000000
VERSION     DASPUL010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830563"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VEJ00_01260"
     CDS             <1..963
                     /locus_tag="VEJ00_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXW99814.1"
                     /translation="QLDDADIRTQATRAQAQVKAAQADETALTTGGTQEEVLTLNAQL
                     VKARSARDTAQRNLDALRRLAQQGAASPGEVRQAEDTLQRAQADLTLLEQKRSERYSH
                     PEVAKIEAQAAEAQAAYDAAEDALSKSSVRAPLDGIVYSLPVKEGAFVQAGDLLLQEA
                     DLSHVLVRAFVDEPDVGRLQAGQKVEVTWDAVPGRTWTGTVSTVPSTVKLRGNRNIGE
                     ATCDVDNHDLRLLPNINVGVTIVTAEHNNVLTVQRDAVHSDDSKPYVFRVVDNQLQRQ
                     PVEISLQNLTRVEITSGLSEGSLVALSAEESKPLYDGAAVRVVP"
     gene            1002..1958
                     /locus_tag="VEJ00_01265"
     CDS             1002..1958
                     /locus_tag="VEJ00_01265"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXW99815.1"
                     /translation="MKLRWLSSLFWFSLLLQPVFAAPSPKELLASGRVDDAIQTLQRQ
                     IEQSPTDAESYNLLCRAYFMLDEWDRGIAACERARNLDPQKSLYYLWLGRTYGEKADR
                     AGFLSAAGLAKKVRTSFERAVELDPKSWEARTDLAEFYLEAPGIVGGGRDKARAQIDA
                     IAPLNPGMAHWVAGRIAEKNKDAAAAEREYRASIAATHSGARAWLDLAIFLRHANRLD
                     EMEYAVANLESCPVDRRESLMDGASVLLRGNRNYPLGIRLLRRYLESPVEEGPAFKAH
                     ELLGQLLEKQGDRTAAAEEYRAALAIAHSYTRAQEDLKRVER"
     gene            2112..3479
                     /locus_tag="VEJ00_01270"
     CDS             2112..3479
                     /locus_tag="VEJ00_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXW99816.1"
                     /translation="MKTPRHAVCSKKWILAAFLLPTSLLAEPVPLKRVVELALSHATG
                     GAIASSDEQKAAAAYRELHDSYIPQLTTGAGLGPPSYGFPLALEGQAPSLFNINMQSA
                     LFNPSLREFVRAAKVDSAVATLKSKDQRNQIIQDAALSYSELAKWEQRLAHLQEVEAE
                     ANKMQAAVAQRVKEGVDTEIDGTRARLSAARVHLRVAEAQGAADVLREHLSKLTGLPA
                     AGIQTDPDSIPAAPAISNDEAVKQATDVSPAVEAAVEHARAQYLRAEAEHKALWPSFD
                     LAGQYALLSKFNNFQNYYIPEKQCAVATPTGSTVTALCSAGGFRQNNATIGVSIRFPI
                     FNASQRARAQAADADALKATKQAEAARNQVSEETLRLQRAVTQMQAARDVAELESEIA
                     EKNMAAAQTRVDAGTATLHELDDARSQASERFIALQDVTFELERAELGLLRSTGDLEK
                     WALGQ"
     gene            complement(3661..4002)
                     /locus_tag="VEJ00_01275"
     CDS             complement(3661..4002)
                     /locus_tag="VEJ00_01275"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXW99817.1"
                     /translation="MTVIDFGGIPIERMPSAEPLLDRALHAIADPTRRRILQALKEGA
                     AKPEGKMRPCLCAGDVEERVQLSQPTISHHMAILTKAGLVEATKQGQWRWYRRNERAI
                     RQVVKMLRGRL"
     gene            complement(4024..4620)
                     /locus_tag="VEJ00_01280"
     CDS             complement(4024..4620)
                     /locus_tag="VEJ00_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXW99818.1"
                     /translation="MTSSQDTEITLGTGKLLVLFFFLVGICALFFALGYSLGRKTESP
                     MAAVSAPTPQPAAPVGKANTAPATQQPMTFYKNVEQTQANPQLTPATETKAETTAAPA
                     NTAPAQTAPTAPAGPPDSMTTLPSSGYFVQVAAVTKQEDAEALVDALKKKDYPAFVAP
                     QTGDKLFRVQLGPYADIKDAEAMRTRLIGDGYSPIVKK"
     gene            complement(4767..5564)
                     /locus_tag="VEJ00_01285"
     CDS             complement(4767..5564)
                     /locus_tag="VEJ00_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HXW99819.1"
                     /translation="MLEMLGGLTTIIIVIFVLYLLSCIKILAEYERGVIFRLGRLQPN
                     ARGPGITFVLAPVERMVRISLRQEALEVPPQDIITRDNVTLKVNAVIFLRVIDPNKAV
                     VEVSNYVYQTSQFAQTTLRSVLGEQELDELLAHRDKINLRLQSILDTHTSPWGVKVSN
                     VEVKQVDLPESMLRAMAKQAEAEREKRSKIIHAEGEYSAAQRLVDAAHLLSGEPVSVQ
                     LRYLQTLTEIGVEKNTTVVFPVPVDFFSGIQKLLATAGGGAAPGKTT"
     gene            complement(5784..6557)
                     /locus_tag="VEJ00_01290"
     CDS             complement(5784..6557)
                     /locus_tag="VEJ00_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99820.1"
                     /translation="MRVLLTALLVSGLSFGQAADVSKPAQGPAAAGSPQSGTAQPAAP
                     ATAAQPPSGTADSKAAVIPAGSKIPLSLAQAISTKNAREGDAVYAQTTFPFVLNDRIL
                     VPAGTYIQGNIRHVEKPGRSKKRAELLIHFTTMIYPSGYTIMLPGAIENTPGADNKSV
                     KDKEGTIQQDKDTGKRIEDAARDAAVAGTVGSIGGAAAGGWNGARYGGLAGIAGGVAW
                     ALLKHGPDVKLEVGTSLEMEIQRDVPVDASRIQVAKAAS"
     gene            6726..>7775
                     /locus_tag="VEJ00_01295"
     CDS             6726..>7775
                     /locus_tag="VEJ00_01295"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HXW99821.1"
                     /translation="MKHFSAVLAAAVLSAASLAVAGLAQDVSGPQTHGIVVANMDRSV
                     KPGDDFFRYANGDWIKRAEIPPDRPSLNVFARLTDLSNKRTADLIEELAKSNSPVGSG
                     TRKVTDLYNSYMNEAAIEAKGLAPIRPHLDAIAAIRNQHQLARALGETLRADVDALNN
                     TNFRTANLFGLWVAPGFNDPDHYTAYLMQGGLELPNREYYLTESEHMRNLRAQYQTHV
                     AAMLRLAGFSDTEARARRIFELEHAIAQTHRTLAENEDIHKANNTWTAADFTSKAPGL
                     DWPEYFRSAGLSKIESFIVWQPEAFTGESALVASTPLDTWKDWLAFHLIEQYADVLPK
                     PVAEERFAFFGKELSG"
BASE COUNT         1548 a         2519 c         2214 g         1494 t
ORIGIN      
        1 cagctcgacg atgccgatat ccgcactcag gcgacccgtg cgcaggccca ggtcaaggca
       61 gcgcaagccg atgagacagc cctgaccacc gggggcacgc aggaagaagt gctcaccttg
      121 aatgcgcaac tggtaaaggc acgcagcgca cgcgacacgg cgcagcgcaa cctcgatgcc
      181 ctccgtcggc tggcgcagca gggtgcggcc tcgcccggtg aagtccgcca ggctgaggac
      241 acgctgcaac gcgcccaggc cgacctcacc ctgctggagc agaaaagaag cgagcgttat
      301 tcccatcctg aagtggccaa gatcgaggcg caagccgcgg aagcgcaggc cgcttacgac
      361 gctgccgagg acgctctcag caagtccagc gttcgcgctc cgctcgatgg cattgtctat
      421 tcgctgccgg tcaaggaggg agcttttgtc caggccggcg acctgcttct gcaggaagca
      481 gatctctcgc acgttctcgt gcgtgctttc gtggacgagc cggacgtcgg ccgcctgcag
      541 gcaggccaga aggtggaagt aacctgggac gcggtccccg gccgcacctg gaccggaacc
      601 gtcagcacag ttccctccac ggtgaaactt cgcggaaacc gcaatatcgg cgaggctaca
      661 tgcgacgtcg acaaccacga tctccgcttg cttcccaaca tcaacgtcgg ggtcacgatc
      721 gtgaccgcgg aacacaataa cgtacttaca gtgcagcgcg acgccgtcca cagcgatgac
      781 agcaagcctt acgttttccg cgtcgtcgac aatcagttgc agcgtcagcc ggtggagatt
      841 tccctgcaga acctcacgcg cgtcgagatt acatcgggcc tttctgaagg atcgcttgta
      901 gcgctgtcag ccgaagaatc caagccgctc tatgatggag cggccgtaag ggtcgtgccg
      961 tagctgctcg ggaaacggca caatccaatt cgtctctgtt catgaaacta cgctggctct
     1021 cttcgctgtt ctggttctcc ctgctcctgc agccggtctt tgccgcgcct tctcccaaag
     1081 agctgctcgc ttcagggcgc gtcgacgatg ccattcagac gctgcaacgc cagatcgaac
     1141 agtctccgac agacgctgaa tcctacaacc tgctctgccg cgcctacttc atgctcgacg
     1201 agtgggatcg cggaatcgcc gcctgtgaac gtgcccgaaa tctcgacccg caaaaaagtc
     1261 tctactatct ctggctgggc cgaacctatg gagaaaaggc tgaccgcgcc ggatttctct
     1321 cagccgctgg gctagcaaag aaagtccgca cgtcctttga acgcgcggtc gaactcgatc
     1381 ccaagagctg ggaagcccgc accgatcttg ccgagttcta tctcgaggcc cccgggattg
     1441 tgggcggagg cagggacaag gcacgggcgc agatcgacgc catcgccccg cttaatccag
     1501 gcatggctca ttgggtagcc gggcgcattg cggaaaaaaa caaagatgca gccgccgccg
     1561 agcgcgaata tcgcgcttcc atcgcggcta cgcactccgg agcccgcgcc tggctggatc
     1621 tcgccatctt cctgcgccat gccaatcggc tggacgaaat ggaatatgcc gtggccaacc
     1681 tcgagtcctg ccccgttgac cgacgcgagt ccttgatgga cggagccagt gtccttttgc
     1741 gtggtaaccg caactatcct ttagggatac gcctcctgcg ccgctatctg gaatcccctg
     1801 tcgaagaggg tcccgcgttc aaggcacatg aactactggg tcaacttctg gagaaacagg
     1861 gagaccgcac ggctgccgcc gaggagtatc gcgccgcgct cgccatcgct cacagctata
     1921 cccgcgccca ggaagacctg aagcgcgtcg agcgttgagc cctgagcctg gctgcggatc
     1981 tccgtgccct tggtggttct cgcaaagctc gatgagccat aaatgtctcc accccttctt
     2041 gcaggcattt agaatttcgc tgaatccgaa tcatgtttga actgatttcc acattgtttg
     2101 agagggacgc aatgaaaacc ccacggcacg cggtatgcag caagaaatgg attctggcgg
     2161 cctttctact ccccacttcc ctgctggcgg agcccgtccc gctcaaacga gttgttgagc
     2221 ttgccctctc tcacgcgacc ggcggcgcta ttgcttcgtc cgacgagcag aaagctgccg
     2281 ccgcctatcg cgagctccat gacagctata tcccccagct caccaccggc gccggcctcg
     2341 gcccgccttc atatggcttt cccctggctc tcgaaggaca ggctccatct ctgttcaaca
     2401 tcaacatgca atcggcgctg ttcaacccct cgttgcgcga attcgtccgg gcggcaaaag
     2461 ttgactctgc cgttgcaacg ctgaaaagca aagaccagcg caatcagatc attcaggatg
     2521 cagcgctcag ctactcagaa ctggcgaagt gggaacagcg cctcgcccat ctgcaggaag
     2581 tggaagcaga agcaaacaaa atgcaagctg cagttgccca gcgggtgaag gagggcgtgg
     2641 acactgagat cgacggaacc cgggcccgtc tttcggccgc gcgcgttcac ctgcgcgtcg
     2701 ccgaggcgca gggagctgcc gacgttctgc gcgaacatct ttccaagctg acgggacttc
     2761 ccgcagccgg cattcagacc gatcccgatt cgatcccggc ggcaccggcc atttcgaacg
     2821 atgaggccgt caaacaagct acggacgtca gccccgcggt agaagccgcc gtggaacatg
     2881 cccgcgcgca gtaccttcgg gctgaggccg agcacaaggc gctctggccc agcttcgatc
     2941 tggccggaca atatgccttg ctctcgaagt tcaacaactt ccagaactac tacatccccg
     3001 agaaacaatg cgccgtggcc acgccaaccg gctccacagt caccgcgctc tgctctgccg
     3061 gcggctttcg tcagaacaat gccacgattg gcgtgagcat ccgttttccg atcttcaacg
     3121 cctcgcagcg agcccgtgcc caggccgccg atgccgatgc cctgaaagct accaagcagg
     3181 cagaggccgc tcgcaaccag gtttctgagg agacactccg cctgcagcgc gccgtcacac
     3241 aaatgcaagc agcgcgcgat gtagctgaac tggagtccga aatcgccgaa aagaatatgg
     3301 ccgcagctca gactcgtgtg gatgccggca cagcaacgct gcacgagctc gacgacgccc
     3361 ggtcccaggc cagcgagcgc ttcatcgcgc tgcaggatgt cacgttcgaa ctcgagcgcg
     3421 ccgagctcgg tctgctgcgc tcaacgggag acttggagaa atgggcgctg ggacaataga
     3481 gcgcagggat gagttccata gcagcggcga ttcgagattt tcgaagtaag attgcagatt
     3541 tgttggacgt gttgtcgatc gcagcggtgt cgacgtaacg tggaagagcg gccctttagg
     3601 gccgcgaaaa gtgccgattc aatggggagc tttagccccg gtggtcgcct cccgctgggc
     3661 ttacagtctc ccccgcagca tcttcaccac ttgccgaatc gccctctcat tgcgccggta
     3721 ccagcgccat tgcccctgtt tggtcgcttc gaccagcccc gccttcgtga ggatcgccat
     3781 gtgatgagaa atggtgggct gcgagagctg gacgcgctcc tcgacgtcgc cggcgcagag
     3841 gcacggccgc atctttcctt cgggtttcgc tgcgccctcc ttgagcgcct gcaggatacg
     3901 gcggcgcgtc ggatcggcaa tggcgtgaag ggctcggtcc agcaatggct cggcggacgg
     3961 catacgctcg ataggaatac cgccgaaatc gatgactgtc aatattgaac ggcgaaaaac
     4021 ccgctatttc ttcacaatcg ggctgtaccc gtccccgatc agacgagtac gcatggcctc
     4081 ggcgtccttg atgtcggcat acggcccgag ttgcacgcgg aacagcttgt cgcccgtctg
     4141 cggcgcaaca aaggccggat agtccttctt tttgagcgcg tctaccaggg cctcggcgtc
     4201 ttcctgtttg gtcacggccg ccacctgcac gaagtatccg gaactcggca gcgtggtcat
     4261 ggagtcgggt gggcctgctg gagccgtcgg tgccgtttgc gcgggagctg tatttgcagg
     4321 cgccgcagtc gtctcggcct tcgtctcagt tgccggggtc agttgagggt tcgcctgggt
     4381 ctgctcaacg ttcttgtaga acgtcatcgg ttgctgggtc gcgggtgccg tgttcgcctt
     4441 gcccaccggc gcagcgggct gcggtgtggg ggcgctgacg gcggccatcg gcgattccgt
     4501 cttgcgcccc agcgaatatc ccaaagcgaa gaacagcgcg cagattccca cgagaaagaa
     4561 gaagaggacc agcagctttc ccgttcccag cgtgatttcc gtatcttgtg acgaagtcat
     4621 tgattcactc ccatgcggcg cacaactagc gccgctcgcc cgaagcgtct ttcatctccg
     4681 ggcacagcga gacctccttt tccgcaagcc gctcgtcgct tcaacaaacg ctgccagaga
     4741 tgatgagacc ctcttttgca ccctggctat gttgtcttcc ccggcgcggc gcctccgcct
     4801 gcagtggcca gcagcttctg gataccggag aagaagtcca ccggcaccgg aaacaccaca
     4861 gtcgtattct tctcgacgcc gatctcggtg agcgtctgca gatagcgcaa ctgcacgctg
     4921 accggctcgc ccgagagcag atgcgcggca tcgaccagcc gttgcgccgc cgaatattcg
     4981 ccctcggcat gaatgatctt cgaccgcttc tcgcgctcgg cctcggcctg cttggccatg
     5041 gcgcgcagca tcgactccgg cagatcgacc tgcttcactt cgacattcga taccttcaca
     5101 ccccacggcg aagtgtgcgt gtcaagaatg ctctgcagcc gcaggttgat cttgtcgcga
     5161 tgcgccagca actcgtcgag ttcctgctcg cccagcactg accgcagcgt ggtttgcgcg
     5221 aactgcgaag tctggtagac gtaattcgaa acttcgacga ccgccttgtt cgggtcaatc
     5281 acgcgcagaa agatcacggc attcaccttc agcgtgacgt tgtcgcgggt aatgatgtcc
     5341 tgcgggggca cctccagcgc ctcctgccgc agcgagatgc gcaccatgcg ctcgaccggc
     5401 gccagaacaa acgtgattcc cggtcctctg gcgttgggct gcaagcgccc caaccgaaag
     5461 atgacgccgc gctcgtactc cgccagaatc ttgatgcagc tcaacagata caggacaaaa
     5521 atgacaataa tgatcgtagt cagtccgccc agcatttcca gcatggacac ctccactggc
     5581 gagcgccgca agcgctccta ccgcggattg tatagcagtc aggtagcctc ggcgcggaat
     5641 tcgccagcgg atggttgctt ggcttgatgc tgcgcagaaa aggcgccgag gcccgatgag
     5701 atagcccggg ctgtttcagc gccgagaaag aacgatcaag gaggaatctc gtccggcgac
     5761 tgaggcgaac cgctcaatcc aacctacgac gctgccttcg ccacctgaat ccggctagcg
     5821 tccaccggca cgtcgcgttg aatctccatt tcgagcgaag tgccgacctc gagcttgacg
     5881 tcgggtccat gtttcagcag cgcccaggcg acacctcccg cgattccggc caggccgcca
     5941 taacgggccc cgttccatcc acccgcggct gcaccgccga tgctgcccac cgtgcccgcg
     6001 actgccgcat cccgtgcggc atcttcgatg cgcttcccgg tgtctttatc ttgctgaatc
     6061 gtaccttcct tatccttgac gctcttgtta tcggcgcccg gcgtgttctc gatcgcaccg
     6121 ggcagcatga tcgtatagcc actggggtag atcatcgtcg tgaaatggat caggagctct
     6181 gcccgcttct tcgagcgccc aggcttctcg acgtgccgta tgttgccttg aatgtaggtg
     6241 cctgccggaa ccaggatgcg gtcattcagc acgaagggaa acgtggtctg ggcatacact
     6301 gcgtcgccct cccgggcatt cttggtggaa atcgcctgcg ccagcgacag cggaatcttc
     6361 gaccccgcgg gaatcacggc cgctttcgag tctgccgttc cagaaggcgg ctgtgccgcc
     6421 gttgccggag ccgccggctg ggctgtgcca gactgcggcg aacctgccgc ggcaggaccc
     6481 tgggccggct tcgaaacatc ggccgcctgt ccaaacgaca gtcctgaaac caggagtgct
     6541 gtcaaaagta cgcgcatccc gtacctccta agtacaccac tgatcccaaa gcttactacg
     6601 gttagtccct aggatgcagt cgtcccaggg ggagttatcc cgccgatcac gcatgctaga
     6661 cttttgcaga ttggcgctac tggtggatcg tttctcaact catcttcccg ctcacggagt
     6721 catacatgaa gcatttctct gcagtcctgg cagctgctgt tctctccgcc gcgagccttg
     6781 cggtggctgg tctcgcgcag gatgtctccg ggccccagac ccacggcatc gttgtcgcca
     6841 acatggatcg ctcggtcaag ccgggcgatg actttttccg atacgccaac ggtgactgga
     6901 tcaagcgcgc ggaaattccc ccggatcgcc ctagccttaa cgtcttcgcg cggctgaccg
     6961 atctcagcaa caagcgcact gccgatctca ttgaggaact cgccaaatcg aattcacccg
     7021 tgggctccgg cacccgcaag gtaactgatc tctacaactc ctacatgaat gaagctgcca
     7081 ttgaggcgaa aggcctggcg ccgatccgtc cgcatctcga tgcgatcgct gccattcgca
     7141 accagcacca actagcccgc gcccttggtg agaccctccg cgccgatgtc gacgccctca
     7201 acaacaccaa cttccgcacc gcgaatcttt tcggactctg ggtggccccc ggctttaacg
     7261 atcccgatca ctacaccgca tacctcatgc aaggcgggct cgagttgccc aatcgcgagt
     7321 actacctcac cgaaagcgag catatgcgca acctccgggc tcaatatcag actcacgtcg
     7381 cggccatgct cagattggcc gggttctcgg atacggaagc gcgcgccagg cgtattttcg
     7441 agctcgaaca cgccattgca cagactcatc gcaccctggc ggaaaacgaa gacattcata
     7501 aagccaacaa cacatggaca gcagccgact tcaccagcaa ggctcccggc ctcgattggc
     7561 ctgaatattt ccgcagcgca ggtctcagca aaatagagag ctttatcgtc tggcaaccgg
     7621 aagccttcac gggcgagtcg gcactggtcg cctccacgcc gcttgacaca tggaaagact
     7681 ggctggcctt tcacctgatc gaacagtacg ccgacgtgct gccaaagccc gtagccgagg
     7741 agcggttcgc gtttttcggc aaggaattgt cgggt
//
LOCUS       DASPUL010000054         3190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1652563, whole genome shotgun sequence.
ACCESSION   DASPUL010000054 DASPUL010000000
VERSION     DASPUL010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1652563"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..485)
                     /locus_tag="VEJ00_01300"
     CDS             complement(225..485)
                     /locus_tag="VEJ00_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HXW99822.1"
                     /translation="MTPRERLTPKEIQVAILVWQGLTNREIGKIIGTSEQVIKNHLRN
                     TFDKLGVWSRLELAMYVASHGGKNWPGASEPADIARKQAIGA"
     gene            complement(482..706)
                     /locus_tag="VEJ00_01305"
     CDS             complement(482..706)
                     /locus_tag="VEJ00_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXW99823.1"
                     /translation="MIDLDQVKVTPRDQEVLNLLVQGCSNKEIAGQLNISPRTVKQHL
                     RTLFLRAGIREGRKRVKLAIAVFAKEEADS"
     gene            complement(703..924)
                     /locus_tag="VEJ00_01310"
     CDS             complement(703..924)
                     /locus_tag="VEJ00_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99824.1"
                     /translation="MLKRRFSVKQLARFETGPAYHGDPEIHMKATAAVQKRNVMKQLA
                     RGLEPARRNGETRAIRKVDCASTKGGRHP"
     gene            1105..2520
                     /locus_tag="VEJ00_01315"
     CDS             1105..2520
                     /locus_tag="VEJ00_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXW99825.1"
                     /translation="MLSHIGQVALASIFILILLAGGPASFAQARSLALSQSGGQRDDQ
                     SVGQTPATGAPLTITLQDALQRARLNDPQYRSAVTDLGLAREDRVQARAGLLPNVNYN
                     NSFIYTQGTGPLPASCTSLTAGCPTSRFIANNGVHEYISQADVHQALSLTTFADYRRS
                     AAALAQARAKAEIATRGLVVTVTQAYYGLVVAQRKYATAQRADAEAQRFLDISVKLEN
                     GGEVAHADVIKARIQAQQQQRDLQESQLDMERSRLDLAVLLFPDFNQDFTVVDDLQAL
                     EPLPTFEEVEAAGKKNNPELRSALAGFEAANHEVTAAWGGLLPALSVDYFYGIDSNEF
                     AIRSHGIRNLGYSTVATLQIPVFSWGADRSKLKQAELRRDQAQVELTHTQKELLSHLR
                     QFYLEAKTARSEMESLASSAEIAAESLRLTTMRYQAGESTVLEVVDAQNTLTQARNSY
                     DDGQARFRVAVANLQTLTGTF"
     gene            2526..>3190
                     /locus_tag="VEJ00_01320"
     CDS             2526..>3190
                     /locus_tag="VEJ00_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HXW99826.1"
                     /translation="MNSFSLIKTSTFNRTARGSLWLTLVAAAGLLLLTSCSKESTNEP
                     VVTVHVAPVEKTTIERTIKAEAILSPRAQAAIVPKISAPVQKFLVKRGSPVHAGELLA
                     VLENRDLAGAAQDTQGAYKQAEAAYETTTAASLPEEIRKAEADAQQAQQVLDAQEKVF
                     QSRQQLFDQGALPRKELDQSRVDITQARNQYDIAKKHLDALMAIGKQQELKAAAGQLE
                     SAKG"
BASE COUNT          634 a         1017 c          878 g          661 t
ORIGIN      
        1 ccagatatag agttaatcgg atcggctccg ggttccgcag gccgcggaca tgaaaccttt
       61 tgcagaattt agctggctct gcaatacacc cccgcttcat ttcctcccca actgcccctc
      121 tctactgcgt attgccctcg agttggttcc tgcgtcggtc aagacacaac atcgcatcgt
      181 accccggcac cgcacaaacc cgagtgcgcg agacaccccc tccgctatgc accgatcgct
      241 tgctttcgag caatatcagc aggttccgat gcccccggcc aattcttccc gccgtggctg
      301 gctacgtaca tggccagctc caagcggctc cagaccccga gtttgtcgaa cgtgttgcgc
      361 agatgattct tgatcacctg ctcgctggtg ccgatgatct tgccaatctc gcggttggtc
      421 agaccctgcc acaccagaat tgccacctga atctcttttg gcgtgaggcg ttctctgggc
      481 gtcatgagtc tgcctcctcc ttcgcaaaca ctgcgatggc cagtttcacg cgcttgcggc
      541 cctcgcggat gccggcgcgc aggaacaggg tgcgcaaatg ctgcttcacg gtgcgcgggc
      601 tgatgttgag ctgacctgcg atctccttgt tgctgcatcc ctgcaccagc agattcaaaa
      661 cctcctggtc ccggggtgtt accttgactt gatccagatc aatcacggat gccttcctcc
      721 ttttgttgaa gcgcaatcga cctttcgaat agcacgggtt tcgccatttc ttcgtgctgg
      781 ctccagcccg cgagctaact gcttcatcac gttgcgcttt tgcacggcag ctgtggcttt
      841 catgtggatt tcaggatcgc cgtggtaggc tgggccagtt tcaaatcggg ccagctgctt
      901 gaccgagaag cgcctcttca gcatcgcctt ttcgcttttt tgtttcctgc gcggattgct
      961 gacgatgggg ctgcgaagcg tatcaggtcg tcacgctccg gttttgttct ttgccgtcat
     1021 acactttcgt ctttgcccaa tcgttctttt tctggaggtg tttgcttgaa ggcgtgtcga
     1081 accggatcat ccagtccccg aagtgtgctt tcccatatcg gtcaagttgc gctcgccagc
     1141 attttcatct tgattctgct ggcgggcggg cctgcgagtt ttgcacaggc tcggtctctg
     1201 gctctgtctc agagcggagg ccagagggac gaccaaagcg tcggccagac ccccgccacg
     1261 ggggctcccc tgacgatcac ccttcaggac gcgctgcagc gggcccggct gaacgatccg
     1321 caataccgat cggcggtcac cgatctcggt ctcgcccgcg aggatcgggt ccaagcccgc
     1381 gccggactgc tgcccaacgt gaactacaac aattctttca tctacaccca gggcacaggt
     1441 ccgcttcccg ccagctgcac ttcgctcaca gctggctgcc ccacttcgag gttcatcgcc
     1501 aacaacggcg tacacgaata catcagccag gccgacgtac atcaggcgct ctctctgacc
     1561 accttcgccg actatcgccg ctccgccgcc gctctcgctc aggcccgtgc caaagctgaa
     1621 atcgccacgc gcgggctggt ggtcaccgta acccaggcct actacgggct cgtggtcgcc
     1681 cagcgcaaat acgcgaccgc tcagcgggcc gacgccgagg cgcagcgttt tctcgatatc
     1741 agtgtgaagc tggaaaatgg aggcgaagtc gcacacgccg atgtcatcaa agcgcggatt
     1801 caggcacagc aacagcagcg cgatcttcag gaatcgcagc ttgacatgga acgcagccgc
     1861 ctcgatctgg cagttctctt gttccccgac ttcaaccagg acttcaccgt cgtcgacgac
     1921 ttacaggccc ttgagccgct gcctacattt gaggaagtcg aagcggcggg gaaaaaaaac
     1981 aacccggaac tgcgttccgc gctggcggga ttcgaagcgg ccaatcacga ggtcactgct
     2041 gcgtggggcg gcctgctgcc tgcgctcagc gtggattact tctacggcat cgattccaac
     2101 gaattcgcca tccgcagcca cggcatccgc aacctgggat actccaccgt ggccaccctt
     2161 cagattcccg tcttcagctg gggtgcggat cgcagcaagc tcaagcaggc tgagttgcgc
     2221 cgcgatcagg cgcaggtcga acttacccac acccagaagg aattgctcag ccatctgcgg
     2281 cagttttatc tcgaagcgaa gacagcgcgc tcggaaatgg agtcgctggc cagttcggcg
     2341 gagatcgccg ccgagagctt gcgcttgacc accatgcgtt atcaggcggg cgaatcgacg
     2401 gtgctcgagg tcgtcgacgc gcaaaacact cttactcaag cgcgtaattc gtatgatgac
     2461 ggccaggcgc gctttcgcgt cgccgtggca aacttgcaga ctctgacagg gactttctga
     2521 ccggaatgaa ctctttctca ctcatcaaaa cctcaacctt taatcgcacg gcccgtggct
     2581 cgttgtggct gacgctggta gctgcggctg gcctgcttct tcttaccagt tgctcgaagg
     2641 aaagtacgaa cgaacccgtg gttaccgtgc acgtcgctcc tgtggaaaag accacaatcg
     2701 agcggacgat aaaggcggaa gcgatcctgt cccccagggc tcaggccgcg attgtgccca
     2761 agatcagcgc cccggtgcag aagtttcttg tgaagcgcgg cagcccggtc cacgccggag
     2821 agttgcttgc ggttctcgag aatcgcgacc ttgcgggtgc cgcacaagac acccagggcg
     2881 cctacaaaca agccgaagcc gcttatgaaa ccaccaccgc agccagcctg ccggaggaaa
     2941 tccgcaaggc agaagccgac gcccagcaag cccagcaggt gctcgatgca caggaaaaag
     3001 tcttccagag ccggcagcag ctctttgatc agggtgcgct gccccggaaa gaactcgatc
     3061 agtcgcgcgt ggacatcacg caggcgcgca atcaatacga cattgccaaa aagcacctcg
     3121 acgcactgat ggccattgga aaacagcagg agctcaaagc ggctgccggg caactggaat
     3181 cagcgaaagg
//
LOCUS       DASPUL010000055         5184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_419336, whole genome shotgun sequence.
ACCESSION   DASPUL010000055 DASPUL010000000
VERSION     DASPUL010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5184)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5184
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419336"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VEJ00_01325"
     CDS             <1..286
                     /locus_tag="VEJ00_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXW99827.1"
                     /translation="AIAHDPATLKRTWEDIKQIMAPGALDPLTKEMIYIAVSVTNQCG
                     YCIASHTVSAQKRGMTDAMFKELMAVVGMANETNKMVTGYQVEIDEQFRK"
     gene            complement(293..808)
                     /locus_tag="VEJ00_01330"
     CDS             complement(293..808)
                     /locus_tag="VEJ00_01330"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXW99828.1"
                     /translation="MKNFFLGSLFTVVIFAVAVLFYLRLGLAEVRGDVPPSRLESSLM
                     RMAVHASVRRNAPEIANPIPPSDENLIAGGKMYLNECAGCHGTPGKARKSDDSLNPPA
                     PQLPTMGTEYSEAQIFWVAKHGIRRTGMFANGVWDSDEKLWTLAAYIYRIRSLPPKVS
                     AEVEKAANTGN"
     gene            complement(850..1233)
                     /locus_tag="VEJ00_01335"
     CDS             complement(850..1233)
                     /locus_tag="VEJ00_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HXW99829.1"
                     /translation="MNRTRRRVARRVDPEKAGSMLLRIERTIRSIPRGKVSTYGGVAR
                     AAGYPGAARQVAKVLRRSFGLPWQRVLGAGGAIKLTGDSAIEQRLRLEAERVGFRGRR
                     VDMKKYEYKFRLSTTTGSLRRKKPR"
     gene            complement(1339..1692)
                     /locus_tag="VEJ00_01340"
     CDS             complement(1339..1692)
                     /locus_tag="VEJ00_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXW99830.1"
                     /translation="MQLKITKRTVDGILAVGLSGRIVFGDESSLLRDEVKKALQDGVK
                     RIVLNLGEVNYIDSGGLGTLVALHTTAHNAGGTIKLANLTKRVGDLLQVTKLLTVFEV
                     HNSEYEALEAFRQVA"
     gene            1883..3736
                     /gene="dnaX"
                     /locus_tag="VEJ00_01345"
     CDS             1883..3736
                     /gene="dnaX"
                     /locus_tag="VEJ00_01345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXW99831.1"
                     /translation="MSYTVLARKYRPQKFSEVIGQEHVTRTLQNAIEQGRTAHGYIFS
                     GHRGIGKTTVARILAMALNCRSKDRPVPEPCGVCESCLEIRNGNSVDVIEIDAATNRG
                     IDEIRELREAARYRPARDRFKIYILDEAHQITDAAFNALLKTLEEPPEHIVFMLATTQ
                     PEDIPQTIRSRCQHFSFRAVKFDDIVGQLRDLVQREKIEADDAALALLAEAGDGSMRD
                     ALSILDQAIASSSGKLTAESVRDLVGAAPAHILEEVMQAVAAGKSDEILRQVDHLISE
                     GHSPTHFARQMVRFLRNATVAKIAGKESALLQISGEERERVARVAELFGEEDLTRHLQ
                     IMLRTHGELGYKQEQRFHLELGLLKMAHAQRLLPIEQLLSEAAGPAQRPSAKPSIVGG
                     APAAEVRRPEAPAARQGFVSPFAADSARKGTPRQEGTVDPLLTPGPRIVAAASQAEPV
                     ILGSAAPDARRDVVTYAREREPQPQAGPQASAVAEDQIEKVQSAVLQALSDGNQRVLA
                     SMLSEGEWSVQAGELIIKVSGSQTAVDMTLGADAKRLIIASASGVMGRAIKLKVVPGA
                     SVAPQENKSNGASVRPTAGGRGRAEQDPVVRRMQEKFGAQIRTVIDYREKR"
     gene            3848..4153
                     /locus_tag="VEJ00_01350"
     CDS             3848..4153
                     /locus_tag="VEJ00_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXW99832.1"
                     /translation="MGSFNLQELMSKAKAQYDSLQKKMQETVVEAASGGGAVTVKMDG
                     RKQVLSLRIDPEAVKAGDVEMLQDMILAAVNEAARKVDAQMQSTVGGMLGGLGIPGL"
     gene            4272..4871
                     /gene="recR"
                     /locus_tag="VEJ00_01355"
     CDS             4272..4871
                     /gene="recR"
                     /locus_tag="VEJ00_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXW99833.1"
                     /translation="MSKFAEPMSRLIDELKKLPGVGAKSAQRLAFHILRSSDEDAEAL
                     AAAVRDVKEKLRLCSVCNNITDVDPCVYCISPTRNQRLVCVVEEPTNIAAIEKTKHFN
                     GVFHVLHGAISPLHGIGPEQLRISNLISRVDAGNVDEVILATNPTVEGEATAVYLSQQ
                     LKGTGVKVTRIAMGIPVGSDIEYTDEITMLKAMEGRREV"
BASE COUNT         1104 a         1546 c         1557 g          977 t
ORIGIN      
        1 ggcaatcgcg catgatcctg ccacgctgaa gcgcacgtgg gaggacatca agcagatcat
       61 ggcgcccggg gcgctcgacc cgttgaccaa ggaaatgatc tacatcgccg tcagcgtcac
      121 caaccagtgc ggctactgca tcgcctcgca tacagtgtcg gcacagaaga ggggcatgac
      181 cgacgccatg ttcaaggagc tgatggcggt ggtggggatg gcgaatgaga ccaataagat
      241 ggtcacggga tatcaggtgg agatcgatga gcagttcagg aagtgacgag cctcagttcc
      301 ctgtattcgc agccttttcg acctcggccg agactttcgg cggcaggctc ctgatacggt
      361 agatgtacgc ggccagcgtc cacagcttct cgtcggaatc ccagactcca ttggcaaaca
      421 tccccgtgcg gcgaataccg tgcttggcaa cccagaaaat ctgggcttcg gaatattccg
      481 tccccatggt cggcagttgc ggagccggtg ggtttagcga gtcgtccgat ttccgcgctt
      541 ttcccggcgt tccgtgacat cccgcacact cgttaagata cattttgccg ccagcgatca
      601 ggttctcgtc ggatggcggg atgggattgg cgatttcggg agcattccgc ctcaccgaag
      661 cgtgcacagc catgcgcata agagacgact ccaaccgcga cggcgggaca tcgccgcgaa
      721 cttcagccag accaagccgg aggtagaaaa gaacggccac agcgaatatc accaccgtga
      781 acaacgagcc aaggaagaag tttttcaagg ggcatcacca agcataggca gggcggaatc
      841 ttacaacgtt cagcgcggtt tttttcttct caagctgcca gtcgtggtgg ataaccggaa
      901 cttgtactcg tatttcttca tgtcgaccct gcgaccgcgg aatccgaccc tctcagcttc
      961 gagccgcaag cgctgttcga tggccgaatc gccggtcagc ttgatggcgc cacccgcgcc
     1021 cagcacgcgt tgccagggca ggccgaagct gcggcggagc accttggcga cctggcgcgc
     1081 cgcgccgggg tagccggcgg cccgggctac gccgccgtag gtcgagactt ttcctcttgg
     1141 aatcgatcgg atggtccgct cgatccgcaa cagcatcgag ccggcctttt cgggatccac
     1201 ccttctcgcg acgcgacgac gggttcgatt cacgtttgaa cgcattccaa gagtcagaac
     1261 ggtcaagaat ccccacccta acgttgcaaa gagcgcaacg ttaggatggg gcaccctttg
     1321 agatgtggct taccgcggct acgctacctg gcggaaggcc tccagtgctt cgtactccga
     1381 gttatgcact tcgaagacgg tcagcagctt ggtgacctgc agcagatcgc cgacgcgctt
     1441 ggtcaggttg gccagcttga tcgtgccgcc ggcgttgtgc gccgtggtgt gcagagccac
     1501 cagcgtgccc agcccgcccg aatcgatata gttcacttcg cccagattga gcacaatgcg
     1561 cttgacgccg tcctgcagtg ctttcttgac ctcatcgcgc agcaatgacg attcgtcgcc
     1621 gaacacgata cggccgctga gaccaacggc gagaatcccg tctactgtcc tcttcgtgat
     1681 cttcaattgc ataacaacgc tcctttgcgg ccgccgattg cccccgcccc agccgcaccc
     1741 gcacttactc tcttaacgat ccagcgtagc gcttgcgacg ccggttcgca accccgagtc
     1801 agcccaactc tccttctgcg aagctgccag catacgcaca gcagttgcga gcttccatcc
     1861 cttataatcg tgtttctcgc tcatgagcta taccgtcctg gcgcgcaaat accgaccgca
     1921 gaaattctcc gaagtcatcg gacaagagca cgttacccgt accctgcaga atgccatcga
     1981 gcagggacgc acggctcacg gctacatatt cagcggccat cggggaatcg gcaagactac
     2041 ggtcgcccgc attctggcca tggcgctgaa ctgccgctcg aaggacaggc ctgtgcccga
     2101 gccctgcggc gtgtgcgaat cctgcttaga aattcgcaac ggaaactccg tggacgtgat
     2161 cgaaatcgat gccgccacga accgcggcat cgacgaaatc cgcgaactgc gcgaggcggc
     2221 gcgctaccgg cctgcccgcg accgtttcaa gatttacatc ctcgacgaag ctcaccagat
     2281 taccgacgcc gccttcaacg ctttgcttaa gacattggag gagccgcccg agcatatcgt
     2341 gttcatgctg gcgaccacgc agccggaaga tattccgcag accatccgct cgcgctgcca
     2401 gcacttcagc tttcgggcgg tcaagttcga cgacattgtg ggccaactgc gggaccttgt
     2461 tcagcgggag aagatcgaag ccgacgacgc cgcgctcgcg ctgctggccg aggccggcga
     2521 cggctctatg cgagacgctt tgtcgattct cgatcaggca attgcgtcgt cgagtggcaa
     2581 gcttacggcc gagtcggttc gcgatctagt cggcgcagca cccgcgcata ttctcgagga
     2641 agtgatgcaa gccgtagcgg cgggcaagag cgacgagatt ctgcgtcagg tcgatcacct
     2701 tatcagcgaa ggccacagcc cgacgcactt cgcccgccag atggtccgtt tcctgcgcaa
     2761 tgctaccgtg gcgaagatcg ccggcaagga gtcagctctt cttcagatct ccggtgagga
     2821 acgggagcgg gttgcacgcg ttgctgaact ttttggcgaa gaggatctga cccgtcatct
     2881 gcaaatcatg ctgcgcacgc atggcgagct ggggtacaag caggagcagc gttttcatct
     2941 ggaactcggg ctgttgaaga tggctcacgc gcagaggttg ctgccgatcg agcaactgct
     3001 cagcgaagcg gccggtcccg cacagagacc ttcagcgaag ccgtcgattg ttggcggcgc
     3061 tccggctgcg gaagtacggc ggcccgaggc gcccgcggct cggcagggct tcgtttctcc
     3121 attcgcggcc gactctgcgc gaaaaggaac gccacggcag gaaggaaccg tcgatccact
     3181 tcttaccccc gggccgcgca tcgttgcggc agccagccag gcggaaccgg tgatattggg
     3241 atccgccgca cccgatgcgc ggagagacgt ggtcacttat gcccgcgagc gcgagccaca
     3301 accgcaagct gggccgcaag cttctgcggt cgcggaagac cagattgaga aagtgcagtc
     3361 ggccgtgctt caggccctga gcgacggcaa tcagcgagtt ctggcctcca tgctttcgga
     3421 gggtgaatgg agcgtgcagg ctggcgaact gatcatcaag gtcagcgggt cacagacggc
     3481 ggtcgatatg acgctggggg cggacgcgaa gcgcctcatc atcgcgtcgg ccagcggggt
     3541 tatgggacgc gcgatcaagc tgaaggtggt tccgggcgca agcgtggctc cgcaagagaa
     3601 caaaagcaat ggggccagcg tgcggccgac cgcgggcggc cgtggccgcg ccgagcagga
     3661 tccggtggtg cgccgcatgc aagaaaaatt tggcgcacag attcgtacgg tgatcgacta
     3721 ccgcgaaaaa cgttgaacca agatactctg tccggtgatc tttgccgcga aggatctatg
     3781 taatttggcg atgcgcgcag actgcctagg ttcttcgctt cgctccgaat gacaacggaa
     3841 aaatcctatg ggtagcttta atctgcaaga gctgatgtcg aaagccaagg cgcagtacga
     3901 ctcgctgcag aagaaaatgc aggaaacggt cgtggaagct gcgtccggcg gaggcgcggt
     3961 cactgtcaaa atggacggcc gcaagcaagt gctcagcctg cgcattgatc ccgaggcggt
     4021 gaaagccggg gacgtggaga tgctgcagga tatgattctc gccgcggtca acgaagctgc
     4081 gcgcaaagtc gacgcgcaga tgcagtcgac agtgggcggt atgctgggcg ggctggggat
     4141 tccgggactg tgaagaattg agaaattggg taatttggta attgggaaat tgaaaatcgt
     4201 ttcctttcgc gcgggcaatc acctgacatt ctcgattaca caattactca atttcccgat
     4261 tacgcaatct tatgtcgaaa ttcgccgaac ccatgtcgcg gttgattgac gagctgaaga
     4321 agctcccggg cgttggcgcg aaaagcgcac agcggctggc gtttcacatc ctgcgctcga
     4381 gtgacgaaga cgccgaagcg ctggccgcgg ccgtgcgcga cgtcaaggag aagctgcggc
     4441 tgtgctcggt gtgcaacaac atcaccgacg tcgatccttg cgtttattgc atcagcccca
     4501 cgcgcaacca gcggctggtg tgcgtcgtgg aagaaccgac caacatcgcc gccatcgaga
     4561 agaccaagca cttcaacggt gtctttcacg ttctgcatgg cgcgatttcc ccgctgcacg
     4621 gcattggccc ggaacagctg cgcatttcga atctcatctc gcgagtcgac gcgggcaacg
     4681 tcgacgaagt catccttgcc accaatccca ccgtcgaagg cgaagcaacg gcggtgtacc
     4741 tctcgcagca actcaaaggc accggcgtga aggttacgcg catcgcaatg ggcattccgg
     4801 ttggcagcga catcgaatac accgacgaga tcaccatgct gaaggccatg gaaggacggc
     4861 gcgaggtgta ggcactcagg ccctgcttcc cactgcacaa gtctgtgtgc aactatgcaa
     4921 gattttgcgg acgaccaatc gccagcattt ccccgagtgt aacgccgaag cacctgcgga
     4981 tcgaatatct acaatcttgt caatgcgtta cggctcgtct cgccaactac agcttgaggc
     5041 taaaaattgg cagaatgcgt gcgagacagg gcttcgagat ttgccagcga tttacgactg
     5101 gccagcgaaa acagcggtca gtcgcgccgg gtgttgatgt aaccgcatcc gcccggcgca
     5161 caagaagccg ggccaaccag cccg
//
LOCUS       DASPUL010000056         4171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_145411, whole genome shotgun sequence.
ACCESSION   DASPUL010000056 DASPUL010000000
VERSION     DASPUL010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145411"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2739)
                     /locus_tag="VEJ00_01360"
     CDS             complement(<1..2739)
                     /locus_tag="VEJ00_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXW99834.1"
                     /translation="MMGQTISHYRITGQLGSGGMGVVYEAQDLTLGRKVALKFLPPQL
                     AQDQNALDRFLLEARAASALNHPNICTIYAVENATNESGEKQSFISMELLEGQSLDQK
                     LRLGPLPTGQLLEIAIQLADALDAAHAKGIIHRDIKPANILLTQRGPVKILDFGLAKL
                     TRREMEMETIGATQDSPAPAHLTSPGATVGTIAYMSPEQARGEELDPRSDLFSLGTVL
                     YQTATGKLPFAGATSAVIFHALLELNPPSVLQLNPALPPKLGEIIEKLFEKDRDLRYQ
                     SAADLRSDLKRLKRDVDSGQKAGSQPPQPAQASAHATAADSALTAAQTPPSSSAVVVA
                     ARQHKFSTALTATIALLVLGAAGYGIYSLVTRSRPAPFQNFSVTKVTDSGNAVSAAIS
                     PDGKYVLILMRSNGLASLWLRNVPTNSNTQVQPPADVYYNGLTFSPDGNYFYFVRSDP
                     GNPELKFLYRAPLLGGAPQKLASDVDSNITLSPDGKLFAFVRMDNPDPGKYRLIVQPA
                     DGGAGDERVLASGPNSQAMYTPAWSPDGKMIVCSRQHATDVWTSMVAIDIASGQQKDF
                     FTSNVQFFKSPGWLPDGSGLLGLARGPETYYHGDQIASVSYPGGKISPVTRDTSSYSD
                     LSIAATGQALAAIQNQERWNLFVMPASGSGAQARSVTAAEAATNFTWTTDGQIVDDQS
                     SLITRIDLTSGNKTVLATEEGKPNGNPAACADGRSIVFELILHGNTESDNIWRIDPAG
                     GNMRQITKGRQDQYPVCSPDSRWVYYIAHGDEGKLARTSIDGGPSQTISKLSITDSVF
                     DISPDGKLAAFGTLAHSGEHKEKLALVSTDTEQAKLLDFERLRFGLLRFSRDGKAVVY
                     PTRDNGVDNLWLQPLDGSKGHTITDFTSERIRDFHWSFDGKQLAMIRGHTDSDVVLL"
     gene            3016..>4171
                     /locus_tag="VEJ00_01365"
     CDS             3016..>4171
                     /locus_tag="VEJ00_01365"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99835.1"
                     /translation="MIGPFRGGRTRAVAGVPSQPNVFYIGQVNGGVWKSDDYGRTWNP
                     IFDSEPTQSIGAIAVAPSDPNIVYVGTGEGLHRPDLSVGNGIYKSTDAGKTWTHLGLN
                     DAQQIPALAVDPRDPNRVFAAVLGHPYGPSEERGIYRSTDGGQSWQKVISRDENTGGS
                     DVEIDPSNPDVVYASMWEAREGPWEDNNDYNGTGGGLFKSTDGGTTWHQLSNGLPRDL
                     SQIYLAIAPSDSRRLYATVAVASGKLGVYRSDDAGDTWAQITTDPRPSGRIGGGDLAI
                     PRVDPKNPDIVTVASTVTMRSTDGGKTWYGFRGAPGGDDYQNLWISPNNGNIILLVSD
                     QGAIITVNGGESWSSWYNQPTAQLYHAIADNSFPYRVCAGQQESGSVCISS"
BASE COUNT          795 a         1238 c         1287 g          851 t
ORIGIN      
        1 aagcagcacg acgtctgaat ccgtatgccc gcggatcatc gccagctgct tgccgtcgaa
       61 cgaccagtgg aagtcgcgga tgcgttccga ggtgaaatcg gtgatggtgt ggcctttcga
      121 cccgtcgagc ggctgcagcc aaaggttgtc cacgccattg tcgcgcgtcg ggtagacaac
      181 ggctttcccg tcgcggctga aacgcagcaa gccaaagcgc agacgctcaa aatcgagcag
      241 tttggcctgc tcggtgtcgg tagagaccag cgctaatttt tctttgtgtt ccccggaatg
      301 cgccagcgtg ccgaaagcgg ccagcttgcc gtcaggggag atgtcgaaca cgctgtcagt
      361 aatcgacaat ttcgaaatgg tctgcgatgg tccgccatcg atggaagtcc gagcgagttt
      421 gccttcgtct ccgtgcgcga tgtagtacac ccagcgactg tcgggcgagc acacgggata
      481 ctggtcctgg cggcctttcg tgatctgccg catattcccg cccgcgggat cgattcgcca
      541 gatgttgtcg ctctccgtgt tgccgtggag gatgagttca aacacgatcg aacggccgtc
      601 ggcgcatgcc gcaggattgc cgttgggctt cccttcctct gttgccaaca ccgtcttgtt
      661 gccgcttgtc aggtcgatcc gggtgatcag gctggactgg tcgtcaacga tctggccgtc
      721 ggtggtccag gtgaagttgg tagcggcttc tgcagcggtg acggaacggg cctgagcgcc
      781 cgatcccgat gcgggcatca caaaaagatt ccagcgctcc tgattttgaa tggccgccag
      841 cgcctgcccg gtcgccgcga tgctgagatc cgagtaactg ctcgtatcgc gggtgaccgg
      901 ggatattttt ccgccagggt acgatacgct cgcgatctgg tctccgtgat agtaggtttc
      961 tggtccgcgt gccaagccaa gcaggccgct cccatccggc aaccatccgg gagacttgaa
     1021 gaactgcacg ttgctggtga agaagtcctt ctgctgcccg cttgcgatat cgatggcaac
     1081 catactcgtc cagacatcgg tcgcatgctg ccggctacag acaatcattt tcccgtcggg
     1141 agaccacgcg ggagtgtaca tggcttggct gttgggcccg ctcgccagaa ctctttcgtc
     1201 acccgcgccg ccatcagccg gctgaacgat cagccggtac ttcccgggat caggattgtc
     1261 catccgcacg aaggcgaaca gctttccgtc gggagaaagg gtgatgttgg agtcgacatc
     1321 ggatgccagt ttctgcggag ccccaccgag cagaggagca cggtagagga acttgagctc
     1381 gggattcccc ggatcgctgc gcacaaagta gaaatagttt ccgtcaggag aaaatgtcag
     1441 gccgttgtag tacacatcgg ccgggggctg cacctgcgta ttgctgtttg tgggtacgtt
     1501 gcgcagccac aggctcgcca ggccgttgct tctcatcaga ataagaacgt acttgccgtc
     1561 gggggagatc gctgccgaca cagcgtttcc agaatcggtc accttggtga cggagaaatt
     1621 ctgaaagggc gccgggcggc tgcgcgtaac cagggaataa attccatacc cagccgctcc
     1681 cagtaccaga agggcaattg tcgcggtcag cgctgtgctg aatttgtgct gacgcgctgc
     1741 cacaaccaca gcggaactgc tgggaggtgt ctgggcagcg gtgagcgcac tatcagccgc
     1801 agtcgcgtgg gcggaggctt gcgcaggctg cggcggctgg gatcccgcct tctgcccgga
     1861 gtcgacgtcg cgcttgagcc gcttcagatc gctgcgcagg tccgccgccg actggtagcg
     1921 caggtcgcga tctttttcga acagcttctc gatgatctcc cccagtttgg gcgggagcgc
     1981 aggattcagc tgaagcacgg aaggcggatt cagctcaaga agagcgtgga aaatcaccgc
     2041 tgaggtcgcg cccgcgaagg gcagtttgcc ggtcgccgtc tggtagagca ccgtgcccag
     2101 cgaaaacaag tcggagcgcg ggtcgagttc ctcgccgcgc gcctgctccg gcgacatgta
     2161 agcgatcgtt ccaaccgtgg cgccggggct ggtcaaatgg gcaggggcag gagagtcctg
     2221 ggttgcgccg atcgtctcca tttccatttc gcggcgagtc agcttcgcca ggccgaagtc
     2281 aagaatcttt accggcccgc gctgtgtgag aagaatattt gccggcttga tgtcacggtg
     2341 aatgatgccc ttggcgtgcg ccgcatcaag cgcatccgcg agctgaatcg caatctccag
     2401 cagttggccg gtcggaagcg ggcccaggcg gagtttctgg tcgaggctct ggccctccag
     2461 caactccatg gagatgaacg attgtttttc gccgctctcg tttgtggcgt tctcgaccgc
     2521 atagatcgtg cagatgttgg gatggttcag ggctgacgcc gcgcgagcct ccagcagaaa
     2581 ccggtcgagg gcattctgat cttgcgccag ctgcggcggc aggaatttca gcgcaacctt
     2641 acggcccaaa gtgaggtcct gggcctcgta taccacgccc atcccgccac tgccgagctg
     2701 gccggtgatg cggtagtgcg agatggtctg gcccatcatt caattgcgga aagcgggagt
     2761 ctcatcttag gtcggtggaa acggtccgtc aaaccgcatt cgtggtccat gacatggcct
     2821 gcagagctgt ggaacgagcc ccggtcactg cggacttggt ttatgctatc tcgtttgccg
     2881 gttcttgctg gggggctcaa attgatgcga acgcatgcag tgcgtcgtct ccttgtttcc
     2941 ttccttctta tcatttctgc cacgccaagt tgggcgcagg ttcctgacaa cgtttatcag
     3001 gagttacact ggcgcatgat cggcccgttt cgcggaggcc gaacccgcgc cgtggctggc
     3061 gtgccaagcc agcccaacgt cttctatatc ggccaggtca acggcggcgt ttggaaatcg
     3121 gacgactacg gccgcacgtg gaatcccatc ttcgatagtg agcccacgca atcgatcggc
     3181 gccatcgccg tcgcgccctc ggatcccaac atcgtctatg tcgggactgg cgaaggattg
     3241 catcgccccg acctgtcggt cggcaacggc atctacaaat caaccgacgc gggcaagacc
     3301 tggacgcatc ttggcctcaa cgacgcgcaa caaattcccg ccctggccgt cgatccccgc
     3361 gatccgaacc gcgtttttgc ggctgtgctc gggcatccct acggccccag cgaagagcgc
     3421 gggatctatc gctcgaccga cggcgggcag agttggcaga aagtcatttc tagagacgaa
     3481 aataccggcg gctctgatgt tgagattgac ccctcgaatc ccgatgttgt ctatgcctcg
     3541 atgtgggaag cccgtgaagg tccgtgggag gacaacaacg attacaacgg cacgggcggc
     3601 ggattgttca aatccaccga cggcggcacc acgtggcacc aactgagcaa tggtctgccc
     3661 agggatctct cgcaaatcta tctggccatc gcgcccagtg actcgcggcg gctctatgcc
     3721 acggtcgcag tggcttccgg aaagctcggc gtatatcgtt ccgacgatgc cggcgacacc
     3781 tgggcgcaga ttaccaccga tccacggcct tcaggccgca ttggcggcgg cgatctcgcg
     3841 atccccagag tcgacccgaa gaatccggat atcgtcacgg ttgccagcac agtcacgatg
     3901 cgttccacag acggtggcaa aacgtggtac ggcttccggg gcgcgcccgg cggcgatgac
     3961 taccagaatc tctggatcag tcccaacaat ggcaacatca ttctgctggt cagcgaccag
     4021 ggcgcgatta tcacagtgaa tggcggcgaa agctggagct cgtggtacaa ccaacccacg
     4081 gcgcagcttt accacgccat tgccgacaat tccttcccct atcgcgtttg tgccgggcag
     4141 caggagagcg gctcggtctg catttcgagc c
//
LOCUS       DASPUL010000057         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1105214, whole genome shotgun sequence.
ACCESSION   DASPUL010000057 DASPUL010000000
VERSION     DASPUL010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105214"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..629)
                     /locus_tag="VEJ00_01370"
     CDS             complement(297..629)
                     /locus_tag="VEJ00_01370"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXW99836.1"
                     /translation="MRTRVVFLALALLSLAGVLAAQNSSAPARPGRKVMLRTAPTYPE
                     LAKRMHVQGAVKIEATVRPNGTVKSTRVIGGSPVLVGAAVDAVSRWKFEAAQTESLEI
                     VQVNFDDQ"
     gene            996..2351
                     /locus_tag="VEJ00_01375"
     CDS             996..2351
                     /locus_tag="VEJ00_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXW99837.1"
                     /translation="MSPSISLPGSQSPFLGSAPQGQAINEVLQIDFLDAINRGLRNNL
                     GLLLASDQTESARGEKWKQLAELLPNVSARVQENVETESLAALGFGKLHFQGTSGFPA
                     VIPAFNYFDARASLGQSLFNFKDIEKERAAAESLTAARYSYKDAREIVVLAVGNSYLE
                     AIAAAARVETAEAQVKNAQALYDKAADQQKAGLSPAIDTLRAQVELQTRQQQLIVARN
                     DLAKQKLSVARIIGLAPGQQFVLTEKAPYQQLAPQPLDTYLQRAYAGRADYQAALAQV
                     RAAELSRRGAAAGRYPSLDFSANLGDIGVTPAHSNGTWQVNGGLNIPIFAGNRVHADV
                     LEADAQLKQARDQLGDLRGRIDYEVRTALLDLNAAAQQVEVARSSVDLAEQALAQSQD
                     RFSAGVADNLEVVQAQEALASAHETYIQSLYAHNLSKVELAYAIGDAEAGVKQYLKGN
                     P"
     gene            2355..>3447
                     /locus_tag="VEJ00_01380"
     CDS             2355..>3447
                     /locus_tag="VEJ00_01380"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXW99838.1"
                     /translation="MAQPAQMPSAEADRPETVSKPLPATEKDFHSLTSRSRSSRFRIV
                     LVVGLVILLVVGVFVYHYVTSYESTDDAQVDGHINSISARISGHVIKLNVEDNQYVQA
                     GTVLVEIDPADYQVAYDRAKADFEDAQAAAVAAGVSVPITSVNTSSQVSATEADVNSA
                     RAGIQVAQQQFEAAKAQLQEAEANNVKAQNDLVRYKQLVDKQEISQQQYDQAVAAAKA
                     SAATVEAARASADAAQQQVTQAQGRLVQAQANWRYANTAPKQIQVTRARAASALAEAQ
                     RKKADLEQAALNLQYTKIIAPVNGIVSDRTVEVGQNVSPGQELMKVINLDDVWITANF
                     KETQLRKMKVGQRVDVEVDANGKTYHGKVD"
BASE COUNT          750 a         1024 c         1069 g          604 t
ORIGIN      
        1 gcccgcacgt tccaaggctg agcttgcagg gaaaaggcaa gcgacacgac ttcacccagg
       61 ttaagttcgg aagcaaccaa aatcccgatt ccggcaaccg acaggtcccg gcaatgcccg
      121 tcgaggcgcc ggggttcttc atccgccaaa agagtcagcg tcacaggaaa ttctgagcgg
      181 tagcggggat gccgccgctt ccggcgctcg gtgctggtga aggctgtttt tgctgattgg
      241 gatgacatcg tcaatgttcg cgaggcttgt tcaatcttgc aggcagaccg cgagctctac
      301 tggtcgtcga agttgacctg gactatttcg aggctctcgg tttgtgcagc ttcaaacttc
      361 caccggctta cggcatcgac cgcggcgccc accaggaccg gacttccgcc aatgactcga
      421 gtagacttga cagtgccgtt gggccgcacg gtggcttcga tttttaccgc accctggacg
      481 tgcattcgct ttgccaattc aggataggtg ggggccgtgc ggagcatgac cttgcgcccg
      541 ggccttgccg gcgcggaact gttctgcgcc gccaggacgc cagcgagcga gagcaacgca
      601 agggcaagaa acacgacacg agtccgcatg caagacggtc cttggttccc acattaccgg
      661 gcgcgtttgc cgcgagcaaa gtactgaagt cactgcacct tggaggaagc taagggttgg
      721 cgcgatcctg gcttgttttc gggtcttcga attctgaacc gtgggccggg catggttttg
      781 catcgcctcc gatcgcgacc gaatcaaatg ctgaaagtta tgctattgac gagaaaggac
      841 gttcccgctt tccgagccgg agttggccgc gggggaacgt ttccgaccag ggaatgaatc
      901 gatccttaaa cccctctcga atggcgttac tctgcctcct catcgcgact ctgtttccag
      961 cgcgagagga ggcgcagacg ccggaacaag gatccgtgtc gcccagcatc tcactgcccg
     1021 gctcgcaaag tccgtttctg ggaagcgcgc cgcaaggaca ggcgataaat gaagttctac
     1081 agatcgattt cctggacgcg atcaatcgtg gtctgcgcaa caatctcggg ctcttgctgg
     1141 caagcgatca aaccgagtcg gctcgaggcg agaaatggaa gcagctcgcc gagttgcttc
     1201 ccaatgtctc cgcacgcgtg caggaaaacg tggaaaccga gagcctcgcg gctcttggct
     1261 ttggcaagct tcattttcag gggacgagcg gcttccccgc ggtgattccc gcgttcaact
     1321 atttcgacgc tcgcgcatcc ctcgggcagt cgctgttcaa cttcaaggac atcgagaagg
     1381 agcgggcggc ggcagaaagc ctgaccgcgg cgcgctacag ctacaaagat gcacgggaga
     1441 tcgttgttct cgcggtaggc aattcgtatc tggaagccat tgctgccgcc gcccgtgtgg
     1501 agactgccga agcccaggtc aagaatgcgc aggccctcta tgacaaggcg gcggatcagc
     1561 agaaagcggg cctgagtccg gccatcgaca cgctacgcgc gcaggtggag ctgcaaacac
     1621 ggcagcagca gctcatcgtt gcgcgcaacg atctcgctaa acaaaagctg agcgtcgcgc
     1681 gcatcatcgg tctcgcgccc gggcagcaat tcgtgctcac cgaaaaagcg ccgtaccagc
     1741 agttggctcc acaaccgctc gatacgtacc tgcagcgcgc ctatgcgggc cgcgccgact
     1801 atcaggcggc cctggcacaa gtgcgagcgg cggaactgtc gcggcggggg gcagcggccg
     1861 ggcgttaccc ttcgctcgat ttcagtgcca atctgggaga catcggcgta acgccggccc
     1921 actcgaacgg aacctggcag gtgaacggag ggttgaacat tcccatcttt gccggcaacc
     1981 gggtgcacgc cgacgtgctt gaggctgatg cccaactgaa gcaggcgcgg gaccagctgg
     2041 gagacctgcg gggccggatc gactacgagg ttcgcacggc gctgctcgac ctgaacgctg
     2101 ccgcccagca agtggaggtg gcacgcagtt ccgtcgacct ggcagaacag gccctggctc
     2161 agtcgcaaga ccgattcagt gccggggtag ccgacaacct ggaggtggtg caggcgcagg
     2221 aagccctggc cagcgcgcac gagacctata ttcagagcct ttatgcccac aatctgtcga
     2281 aggtggagtt ggcctatgcc atcggcgacg ccgaggcggg cgtcaaacaa tacttgaaag
     2341 gcaatcctta atttatggca caacccgctc aaatgccttc cgctgaagcc gaccggccgg
     2401 aaaccgtgtc caagccgttg ccggccacgg aaaaggactt tcacagcctg acctcacgca
     2461 gccggagttc ccggtttcgg atcgtattgg tggttgggct ggtgatcctg ctcgtcgtcg
     2521 gagttttcgt ctatcactac gtcaccagct atgaatcgac cgacgatgcg caagtcgacg
     2581 ggcatatcaa ttccatcagc gcgcgcatct ccggccacgt catcaagctc aacgtcgaag
     2641 acaaccagta tgtgcaggct ggaacggtgc tggtggagat tgatcccgcc gactatcagg
     2701 tggcctatga ccgggccaaa gcggatttcg aagatgccca ggcggccgct gtggccgccg
     2761 gagtgagcgt gccaatcacg tcagtgaaca cgagcagcca ggtttcggcg actgaggccg
     2821 acgtgaacag cgcccgggcc ggaatccagg tggcacagca gcagttcgaa gcagccaaag
     2881 cacaactgca ggaggccgaa gccaacaacg taaaagcgca gaatgacctg gtccgctaca
     2941 agcaactggt cgataaacaa gagatctcgc agcagcagta cgaccaggcc gtggccgccg
     3001 ccaaggccag cgcggctaca gtggaggcgg cgcgggccag cgccgacgct gcacagcagc
     3061 aggtcacgca ggctcagggc agactcgtgc aggcgcaggc aaactggcgt tacgccaaca
     3121 ctgcacccaa gcagattcaa gtgacgcgcg ccagggcggc ctcggcgctt gcggaggcgc
     3181 agcgtaagaa agccgatctg gagcaggctg cgttgaacct ccagtacacg aagatcatcg
     3241 cgccggtgaa cggtattgtg agcgaccgca ccgtagaagt cggacagaat gtttcgccgg
     3301 ggcaggagtt gatgaaggtc atcaatctcg acgacgtctg gattaccgcc aacttcaaag
     3361 agacccagct ccgcaaaatg aaagtcggcc agcgcgtcga tgtcgaggtc gacgcgaacg
     3421 gcaagacgta ccacggcaag gtcgaca
//
LOCUS       DASPUL010000058         4177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1653494, whole genome shotgun sequence.
ACCESSION   DASPUL010000058 DASPUL010000000
VERSION     DASPUL010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653494"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2669)
                     /locus_tag="VEJ00_01385"
     CDS             complement(<1..2669)
                     /locus_tag="VEJ00_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXW99839.1"
                     /translation="MSFLDQLNPQQREAVETTEGPVLILAGAGSGKTRVITYRIAHLI
                     ENLGVMPESILAMTFTNKAAAEMGERVDKLVGGLSIAKPVISTFHSFCVRVLRRDIEA
                     MRIPSTVPGQPPIGHTKNFVIYDEADQLSVVKSIMKRLGLDDKQLTPRTVLGRISWAK
                     NHMLDPQELYLQSADPKTERIAHLYEEYRKELRKANALDFDDLLLEAMRLLKVAAPVR
                     DYYNRRFQYLLIDEYQDTNRPQYELMRLLAGTRHNVCAVGDEDQSIYSWRGADIRNIL
                     EFEQDFPEAKIIRLEQNYRSTQNILQAASSVVANNIRRKGKNLWTSRQGGAKLGYYEA
                     PDGENEALFAADWIARYVREAVEGGQNPRAAVLYRTNSQSRLFEEAMRRYGLKYHVVG
                     GFSFYERAEIKDMISYLKVIQNPDDSISLLRVINTPARGIGKGTIDTLEQLALETGLS
                     LWGAIGEAVRRQLVPPRALSSLKSFQHLIEDARAMRAGTFVEQLEQSADGAEAERKPE
                     AASAVWGRAPSPVQRSGAPQPSFEAEDLAAPDDATAFDPAEFGNFSFDFGAAGPANPA
                     EAGSLRDSHRDAGAATDGADVASNPDAFPTPTVSTADLLKFLIDRTGYIKLLEEEDTP
                     EAWSRIENLRELVNAAMDSRDRGETLDQFLDHAALIADADDYDERAQITLMSLHAAKG
                     LEFPLVFLSGLEEGLFPHSRTMLVPEDIEEERRLCYVGMTRAMDQLILTRAVYRRRYG
                     TDLPEASVPSRFLEEVPGQLIEELGTRRGSLRSLDGRGARPHTAFAGSATESTHYSYE
                     DEDQSAAWHAQTDGRTASGASKVQRATPASRYNSIENIAEFFASRGRKFTVPKVPVEE
                     PTGKRGFRPGQKVRHPKYGEGTVYQREGEGEEA"
     gene            complement(2770..3618)
                     /locus_tag="VEJ00_01390"
     CDS             complement(2770..3618)
                     /locus_tag="VEJ00_01390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXW99840.1"
                     /translation="MQHGQFSKTAHGVAVRRAAHQLLDQPRVHDDPLALAMIGREAAL
                     SLQRELHKQEDALARALRAFLAARGRYAEDQLKQARSRGVTQYVVLGAGLDTFAFRNP
                     HQDLRVFEVDHPATQEWKQERLREAGIPVPPSVSFVRVDFEEQSLRAALELAGFIAEQ
                     AAFFSWLGVTPYIKRESCMATLGFIATMPRGSGVVFDFAVHSDLLEHEQRVALSALSA
                     RVAAAGEPFQLFFHPEELSGALRAQGYREVELLAGEQINALYFKDRSDGLQVSGNIGR
                     LLCAWV"
     gene            complement(3622..>4177)
                     /locus_tag="VEJ00_01395"
     CDS             complement(3622..>4177)
                     /locus_tag="VEJ00_01395"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HXW99841.1"
                     /translation="GSPKLRDRGGGRFLAPLVKARGFGMTPFDWSSRGEPPSWRSCDT
                     ESMQSPLQSPTAATDPRSVKVNLTTGTGMDIEWGDGHVSHYNFVYLRDACPCAMCEEE
                     RAKTGRRPGDAPKPAPGTLPMFKPAAKPISAEGVGKYAIRFAWNDNHDLGIYSWRFLR
                     EVCPCDACKAAREPQTENSVPSVQ"
BASE COUNT          747 a         1295 c         1308 g          827 t
ORIGIN      
        1 ttggcttctt cgccttctcc ttcgcgctga tacaccgttc cctcgccgta tttggggtgg
       61 cgaaccttct gcccgggacg gaatccgcgc ttgccggtgg gctcttcgac gggaactttc
      121 ggcacagtga acttcctgcc gcggctggcg aaaaactcgg cgatgttctc gatcgagttg
      181 tagcggctcg caggagtcgc cctctgcacc ttcgacgcgc cggacgcggt ccggccgtcc
      241 gtttgtgcgt gccaggccgc actctggtct tcgtcttcgt aggaatagtg cgtcgattcc
      301 gtcgcggagc cggcaaaggc cgtgtgggga cgggcgcctc gcccgtccag cgagcgcagc
      361 gagccccgtc gcgtcccgag ctcttcgatc agttgtcccg gcacttcttc gagaaaccgc
      421 gaaggcacgc tggcttcggg caaatcagtc ccgtagcgcc gccggtaaac cgcgcgcgtg
      481 agaatgagct gatccatggc gcgagtcatg cccacgtagc aaagccgccg ctcttcctcg
      541 atgtcttccg ggacgagcat ggtgcgcgag tggggaaaaa gtccctcctc cagcccgctt
      601 aggaagacca gtggaaactc cagccctttg gcggcgtgca ggctcatcag cgtgatctgc
      661 gctcgctcgt cgtagtcgtc ggcgtcggcg atcagcgccg cgtggtcgag gaactggtcg
      721 agcgtctcgc cgcggtcgcg cgagtccatg gcggcgttga ccaattcgcg cagattttcg
      781 atgcgcgacc acgcttccgg cgtgtcttct tcttcgagca gcttgatgta acccgtgcga
      841 tcgatcagaa atttgagcag gtcggcagta gaaacggtgg gtgtggggaa cgcatcgggg
      901 ttggacgcaa catcagcacc gtcggttgca gcgccggcgt cccggtggct gtcgcggagg
      961 ctgccagcct ccgcagggtt ggcaggaccg gctgcgccaa agtcgaacga gaagttgccg
     1021 aactcggcgg gatcgaacgc agtcgcgtcg tcgggagcag cgagatcctc ggcttcgaag
     1081 gatggctgag gagctccgct ccgctggacg ggcgagggcg cgcgtcccca cacggccgat
     1141 gccgcttcag gttttcgctc ggcttccgct ccgtctgcag actgttccaa ctgctccacg
     1201 aacgttcccg cccgcatcgc ccgcgcgtcc tcgatgaggt gctgaaagct ttttagcgac
     1261 gacaacgccc ggggcggcac aagctggcgg cggacggcct cgccaatcgc gccccatagc
     1321 gacaatcccg tttcgagagc gagttgctcc agcgtgtcga tcgtgccctt gccgatgccg
     1381 cgcgcgggag tgttgatcac gcgcagcagc gaaatcgagt cgtcgggatt ctggatcact
     1441 tttagatatg agatcatgtc cttgatctcg gctcgctcgt aaaaagaaaa tcctccgacc
     1501 acgtgatatt tcagcccgta gcgccgcatc gcctcttcaa acaggcgcga ctgcgaattg
     1561 gtacggtaga gcacggccgc cctcggattc tgaccgccct cgactgcctc gcgcacgtag
     1621 cgggcaatcc agtccgcggc aaacagcgct tcgttctcgc cgtccggggc ctcgtagtag
     1681 ccgagcttcg ctccgccctg gcgcgatgtc cagaggttct tacctttgcg ccgaatgttg
     1741 tttgccacca ctgaagatgc cgcctgcaga atgttctgcg tcgagcggta gttctgttcc
     1801 agccggatga tcttcgcctc cgggaaatcc tgttcgaatt cgagaatgtt gcggatgtcg
     1861 gcgccgcgcc aggaataaat cgactgatcc tcgtcaccca ctgcgcacac gttatggcgc
     1921 gtgcccgcca gcaggcgcat gagttcgtac tgtggccggt tcgtgtcctg atattcgtcg
     1981 atcaagagat attgaaagcg ccggttgtag taatcccgca ccggcgcggc gaccttcagc
     2041 agacgcatcg cttcgagcag cagatcgtcg aagtcgagcg cgttcgcctt gcgcagttcc
     2101 ttccgatatt cctcgtacag gtgcgcgatc cgctcggtct tgggatcggc cgactgcagg
     2161 taaagttcct ggggatcgag catgtgattc tttgcccacg aaattcgccc cagcacggta
     2221 cgcggcgtga gctgcttgtc atcgagcccg agccgcttca tgatcgactt gacgacagag
     2281 agctggtcgg cttcgtcgta gatgacgaaa ttcttggtgt ggccgattgg cggctgtccc
     2341 gggacggtcg acggaatccg catagcttca atgtcgcgtc gcagcacgcg cacgcagaag
     2401 gaatggaacg tcgagatcac cggcttggcg atgctcagcc cgccgacaag cttgtcaacg
     2461 cgctccccca tttccgcggc ggctttgttg gtgaaggtca tcgccaggat ggattcgggc
     2521 atcacaccca gattctcgat cagatgcgcg atgcggtagg tgattacccg cgtcttgccg
     2581 ctgcctgcgc ctgccaggat cagcaccgga ccttccgtgg tctcaacggc ctcgcgctgc
     2641 tgcgggttga gttggtcaag aaaagacaat ggggaaacgg ctccggatgt gctgattaaa
     2701 ttttacaggg gaatcgacgg acacggattt ccgattggcg acggcccaag gtgaaccgcg
     2761 gcgaacggcc tacacccagg cgcacaacag ccgtccaatg ttgccgctga cctgcaagcc
     2821 atcgctgcgg tctttgaagt agagggcgtt gatttgttcg ccggccaata gttcgacttc
     2881 gcggtatccc tgggcccgga gggccccgct aagttcctcc ggatgaaaga aaagctgaaa
     2941 gggctcaccg gctgcggcca cgcgtgccga aagggccgag agcgcgaccc gttgctcgtg
     3001 ctcgagcaag tccgagtgca cggcaaagtc aaacaccacg ccgctgccgc ggggcatggt
     3061 cgcgatgaat cccagtgtag ccatgcagga ctcacgcttg atgtaaggtg tgactccaag
     3121 ccaggagaaa aaggccgcct gctctgcgat aaaacccgcc aactccagag cggcgcgcag
     3181 ggattgctct tcgaaatcaa ccctcacaaa gctcacagac ggtggcaccg gaatgccggc
     3241 ttccctgagc cgctcctgct tccactcctg cgtcgcgggg tggtcgactt caaaaacccg
     3301 caggtcctgg tgcggattgc ggaacgcgaa tgtatcgaga ccggcgccca gcactacgta
     3361 ttgcgttaca ccacgtgagc gtgcctgctt gagctgatcc tcggcatagc ggccacgcgc
     3421 ggccagaaac gcgcgcaggg cgcgcgccag cgcatcttcc tgcttgtgca gctcgcgttg
     3481 cagagacagg gctgcctccc ggccgatcat ggccaatgcc agcggatcgt cgtggactcg
     3541 tggctgatcg agcaactggt gggcggcgcg gcgaaccgcg actccgtggg cagtcttgct
     3601 gaattggccg tgttgcataa gtcactgaac ggaaggcacg gaattctcgg tttgtggttc
     3661 cctggcggct ttgcacgcgt cgcaggggca aacttcgcgc aaaaatcgcc aagagtatat
     3721 cccaagatcg tggttgtcgt tccaggcaaa ccggatggcg tacttgccca cgccctcagc
     3781 cgatatcggc tttgcggccg gcttgaacat cggcagagtc ccgggcgcag gcttcggcgc
     3841 atcgccggga cgccgtcccg tcttggcgcg ttcttcttcg cacatggcgc aagggcaggc
     3901 gtcgcgcaga tagacgaagt tgtagtgcga aacgtgcccg tcgccccact cgatgtccat
     3961 gcccgtgccg gtggtgagat tcaccttcac cgacctaggg tcggtggcgg ccgtgggcga
     4021 ttgcagcggg gactgcatgg attcagtatc gcacgagcgc caggaaggcg gttccccgcg
     4081 tgaactccag tcgaagggcg tcattccgaa gccccgcgct ttcaccagcg gggcgaggaa
     4141 tctacccccg ccccggtccc gcagcttagg agatcct
//
LOCUS       DASPUL010000059         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1516730, whole genome shotgun sequence.
ACCESSION   DASPUL010000059 DASPUL010000000
VERSION     DASPUL010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516730"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VEJ00_01400"
     CDS             <1..219
                     /locus_tag="VEJ00_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99842.1"
                     /translation="SRLPGIFDVAESVLRRAVRLSWPAPLAKMPATLSEPEFATVLGM
                     VNYGQRARVARGLQESRWGTKLKAMLVG"
     gene            524..1840
                     /gene="ftsZ"
                     /locus_tag="VEJ00_01405"
     CDS             524..1840
                     /gene="ftsZ"
                     /locus_tag="VEJ00_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXW99843.1"
                     /translation="MSNKSNIRISFNEEPKSDAKIKVIGVGGGGGNAVNRMIDAGVEG
                     IEFITANTDLQALRMSQAPVKLQLGVKLTNGLGAGANPEVGRKAALEDSDKIIEALEG
                     ADMVFVTTGLGGGTGTGAAPIIASLASEMGALTVAVVTKPFSFEGKRRMQQAERGISE
                     LMESVDTTIVIPNEKLLAVAEDAGFFESFRIADDILRQGVQGISDIITIPGIINRDFA
                     DVKTIMSGMGYAVMGTASASGAKRTIEAAQKAIASPLLEAGAIDGARGILINITGSSS
                     LKLAEVQQACSIIQSAAHEDANIIFGAVMDEKMKDSVKITVIATGFREVPSGKRRHQE
                     MHTSFSSSHAAALEDPMDFPDAPGPRETPPEPQRIMSEPVMERSVTDERVYDDGAAAM
                     AVHDRPANPIPLDAVSLDSVRNTMVTNFEHDDLDVPAFLRKRNEVM"
     gene            2265..3485
                     /locus_tag="VEJ00_01410"
     CDS             2265..3485
                     /locus_tag="VEJ00_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXW99844.1"
                     /translation="MPRGTSLFRLLVGILVWLPLASLSWAAHITTHAPNKSKSARKIS
                     ETQHTRRRLHHLARSRAAVTPVSVSTRRHRYHERFHMSSFADDIAAGDLTAGEDPIVR
                     QAAIDALGNMNGTVVAIEPTSGRVLAMVNQKLALSSGAQPCSTIKLSVALAALNEGLV
                     SKETEVALGRRYHLNLTEALAHSNNAYFEALGRELGFEKVAYYAHEYGLGELAGYDIP
                     GEQTGTYPEEVIPSKLGGVGKMCSFGEGISMTPLQLGAMVSAIANGGTLYYLQHPTTA
                     EEVANFQPRVKRQLDIAPLIPEISDGMAGAVEYGTARSLRLNFNEEEILGKTGTCSHA
                     GTRYGWFASYANTNVGRIVVVVFLEGGRPTFGPKAAEIAGRMYRSMYDHNFFVAGPGA
                     PADQAAAKPDTATP"
BASE COUNT          789 a         1053 c         1230 g          717 t
ORIGIN      
        1 tcgcggctcc cgggaatctt cgacgtggcc gaatcggtgc tgcggcgtgc ggtgcggctt
       61 tcgtggccgg ctccgctggc gaagatgccg gcgaccttgt cggagccgga gtttgcgact
      121 gtgcttggga tggtgaatta cggtcagcgc gcccgcgtgg cgcgcgggct tcaggaatcc
      181 cgatggggaa cgaaattgaa ggcgatgctg gtggggtgaa ggagcagggg ctaggggtca
      241 ggggctagtg atcagtggtc agtgcaaagt tgtttgacgg ttttcttggc gaactttgcg
      301 gccgttcttc gcgggctttg cggttcaggg ttctgcagga gccggcagaa aagctaaacc
      361 gcaaagtgcg ctaagaactt cggcggagaa aggctacgga gattgggatt ggcagtgatc
      421 aatacgtggg cggggcttgc tgaccctggc ctctggtccc tgaccccttc tggccaaggt
      481 tattcacgta agaaggaaga gaatccaatt caggtggaca caaatgagca acaaatcgaa
      541 cattcgcatc agcttcaatg aagagccgaa gagcgacgcc aagatcaagg tcatcggcgt
      601 gggcgggggc ggcgggaatg cagtcaaccg aatgattgat gccggagtcg agggcatcga
      661 gttcattacc gccaacacgg acctgcaggc gttgcgcatg tcgcaggcgc cggtgaagct
      721 gcagctcggc gtgaagctca ccaacgggct gggcgcgggg gcgaatccgg aagtcggacg
      781 caaggccgcg ctcgaggatt ctgacaagat catcgaagcg ctcgaaggcg ccgacatggt
      841 gttcgtcacc acgggcctgg gcggcggcac gggcacgggc gcggcgccga tcattgcctc
      901 gctggcaagt gaaatgggcg cgctcacggt cgcggtcgtc accaagccgt tttcgttcga
      961 gggcaagcgg cgcatgcagc aggccgagcg cggcatctcg gagctgatgg aatcggtcga
     1021 cacgaccatc gtcattccca acgaaaagct gctggccgtg gccgaagatg cgggcttctt
     1081 cgaatcgttc cgcatcgccg acgatattct gcggcagggc gtgcagggga tctccgacat
     1141 catcacgatt cccggcatca tcaaccgcga cttcgccgac gtaaagacga tcatgtcggg
     1201 catggggtat gcggtgatgg gcacggcgtc ggcaagtggg gccaagcgca cgattgaggc
     1261 cgcgcagaag gccatcgcgt caccgctact cgaagcggga gcaatcgacg gagcgcgcgg
     1321 gattctgatc aacatcacgg gttcgtcgtc gctgaaactg gcggaggtgc agcaggcgtg
     1381 ctcgatcatc cagagcgcgg cgcacgagga tgccaacatt attttcggcg cggtgatgga
     1441 cgagaagatg aaagacagcg tgaagatcac tgtgatcgcc acgggattcc gcgaagttcc
     1501 atcggggaag cgccgccatc aggagatgca cacgtcgttc tcatcgagcc acgccgcggc
     1561 gctggaggat cccatggatt ttcccgatgc gcctggtccg cgcgaaactc cgccggagcc
     1621 acagcgcatc atgtccgagc ccgtgatgga gcgcagcgtc accgacgagc gagtctatga
     1681 cgacggcgca gcggcgatgg cggtgcacga ccgcccggcc aatccgattc cgctcgacgc
     1741 cgtttcgctc gacagcgtgc gcaacaccat ggtcacgaac ttcgagcatg acgatctcga
     1801 cgtgccggcg ttcctgcgca agcgcaatga agtaatgtaa gtagtcccaa gctgccagtc
     1861 ccaagtccca aggttgggat cattggtgcg ggatcattga attgatgagc agcgaattgc
     1921 gtgcgatgtg caacggcttg aagctggctt gcgacttggg acttgcaact tgcgactaga
     1981 gggctcgcga ctaggaaaga attttcaccc gggggaagta tctttccggt aacggggtaa
     2041 tgggttaccg aaggcgctcg aaccgtagta atctcacgat atgtgcttgt ttcataatgg
     2101 gttgaagcga aattaatgtt tgcagaaatc gaaactttac cattgccaga ttcatgctaa
     2161 gtctcatact atcgcagaga taagccataa tcggtggggt tactgccggg ctggcacgct
     2221 tcgtgcatcg aagtaaacag atttggggga gtggaatcag aaagatgcct aggggaacga
     2281 gcctttttcg tctgctggtt ggaattctcg tatggctgcc gctggccagt ttgagctggg
     2341 cggcgcacat cacgacgcac gctcccaaca aatcgaagtc ggcgcgaaag atcagcgaaa
     2401 cgcagcacac gcggcgtcgc ctgcatcatc tggcgcgcag ccgggcggcg gttacacccg
     2461 tctcggtgtc gacacgccgt caccgctatc acgaacgttt tcacatgagc tcgttcgccg
     2521 atgacatcgc agcgggcgac ctcacggccg gcgaagatcc gattgtgcgc caggccgcga
     2581 tcgatgcgct gggcaacatg aacggcaccg tggtcgcgat cgagccgacc agcgggcgcg
     2641 tgctggccat ggtcaatcag aagctggcgt tgtcgagcgg agcgcaaccc tgctcgacca
     2701 tcaagctctc ggtcgcgctg gccgcactga acgaaggact ggtctccaag gagaccgaag
     2761 tcgcactcgg ccgccgctat cacctgaacc tgaccgaagc gctggcgcac tccaacaacg
     2821 cttacttcga ggcgctgggc cgcgagctgg gatttgaaaa agtcgcctat tatgcccacg
     2881 aatacgggct gggcgaactc gcgggctatg acattccggg cgaacagacg gggacgtatc
     2941 ccgaagaggt gattcccagc aagctgggcg gcgtaggcaa gatgtgctcg ttcggcgagg
     3001 gcatctcgat gaccccgctg cagctgggcg cgatggtctc ggcgatcgcc aacgggggca
     3061 cgctctacta cctgcagcat ccgacgactg cagaagaagt cgcgaatttt cagccgcggg
     3121 tgaagcggca gctcgacatc gctccgctga ttccggaaat ttccgacggc atggcgggcg
     3181 cggtggaata tggcaccgcg cgcagcctgc gcctgaactt caacgaagaa gagatcctgg
     3241 gcaagacggg aacctgttcg cacgccggga cgcgttacgg ctggttcgct tcctacgcca
     3301 acacgaacgt gggacgcatc gtggtcgtgg tcttcctcga aggcggacgg cctactttcg
     3361 gccccaaagc ggctgagatt gccgggcgca tgtaccgcag catgtacgat cacaacttct
     3421 tcgtcgccgg acccggggcg ccggcggatc aggccgcggc caagccagac accgctaccc
     3481 cgtaattcga tcacaacttt tcctgaagag gcgcggcctg gctgcgcctt cttcgttttg
     3541 ggcacgatct tcgtagggtc acgttaacgc tcgctcactc gaatacggct ttagcgacct
     3601 ctaatgcagg agtcagagtc gagcgagctt ttcgcttccg acgtcacagc gattcctcac
     3661 cagaatcgac tttctttttt tcctgccgat aaggaatgaa gtgggagaac tacgtcagca
     3721 acgatgatgc cgcaggaagt tttcgactgg tggttgatga tttcggcctg gctgggaatg
     3781 agctcggcg
//
LOCUS       DASPUL010000060         4724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_968760, whole genome shotgun sequence.
ACCESSION   DASPUL010000060 DASPUL010000000
VERSION     DASPUL010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4724)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4724
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968760"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4724
                     /locus_tag="VEJ00_01415"
     CDS             <1..>4724
                     /locus_tag="VEJ00_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HXW99845.1"
                     /translation="GASTFVKFCKIGALSATGTRPYADGADGFVMGEGAAIFLLKWLA
                     DAERDGDKIYAVLRGIGASSDGKGKGITAPNPIGQKLCFERAWENAGLPAATATLIEG
                     HGTSTRVGDVVEAQSLAEVLSVHHLPAHSVALGSVKSNIGHLKGAAGAAGLLKTALAL
                     RDKLLPPSVHCEHPSADIDFAHSPLYVNTELKPWAAPKDFPRRAGVSAFGFGGTNFHM
                     VLEEHIPHRLTGNGRRSVAVATIPETAIPEVTMKAKESPGASEYAAISSHAPCLVGAR
                     KAPLRGAVVIGADSEAALAERLRTVWKDAQAGRTPGLGAPAEADLRAPYRLAIDYIDA
                     ADLADKSSKALKALTTNQPAVWKALRAQGVFRGQGPAAKVAFLYTGQGSQYVNMLAGL
                     GAAEPIVGDTFAEADRVMTPLLGKPLSNFIFVDPSNLNAVAKAEDDLRQTAITQPAVL
                     AADLALTRLLAAYGVEPDFTMGHSLGEYGALVASGALPFADALEAVSARGREMTRFSL
                     ADNGRMAAVFAPLPEIERLLKTIDGYLVIANINSDHQAVIGGASTAVERAIEVFQKAG
                     YEVAALPVSHAFHTSIVAPASEPLRRTLERLHLQSPRVPIVANVNGEFYPQGPDVVPQ
                     MLDILAKQVAAPVQFVKGLRTLYEAGVRVFVEVGPKKALQGFAEDVLGERGDVVSFFT
                     NHPKFGDVPSFNQALCGLYAAGLGRGTGDAVAASARGETNVLTPALPASFEAPRQSAR
                     LASAPGIAPAGVKASGDHFEELGRFFADVLERGWKIQGGREHVSSTEPVVITGAALGL
                     PGTQQIFDDDNLARILRGDQFIDLIPARFRHAMLDKHINRLVKTDSGGPTFETITNVA
                     DVIKLAGRAGAFDLEKEFGVPAERIAALDRVSQLAIAVGIDALRDAGIPLVMRYKTTS
                     KGTKLPDRWTLPDSLRDDTGVIFASAFPGYDSFADEMRRYYTDHSRREQLSGLESLMA
                     RAKGNGDSGLEQEIERRIEDLRDAIEKEPYVFDRRFLLRTLAMGHSQFAEFIGARGPN
                     TQINAACASTTQAVGLAEDWIRAGRCRRVIVISADDITSDHLMDWFGAGFLASGAAAT
                     DEVVEEAATPFDRRRHGMIVGMGAAALVVESAQAARQRGLQPICEVLSAVTANSAFHG
                     TRLDVQHIGEVMEELVSRAESRGISRHQIAPHTVFVSHETYTPARGGSASAEINALRR
                     VFGKAADQIVIANTKGLTGHAMATGIEDVLAVKTLETGVVPPVANFKEVDPELGTLNL
                     SKGGVYPVEYALRLGAGFGSQISMTLLRWLATKDGVHRRPNALGYAYRIVDEKCWRAW
                     LSQTAGRPSADLEVAHRTLRVRDRRPAMAGVTELAPRVEAASAASPVQTIKPLAEVPA
                     THRPAVLAVSKVDIANVVTEPPTPISAPMSPSVVRPSAPPLQKPAAEGDPVKERILAL
                     VAEKTGYPVDMLDLDLDLEADLGVDTVKQAEVFATIREAYSIVRDDKIRLRDFPTLAH
                     VIRFVHERRSDLGPEPAGLPVAKPETAFAAAVPEPPLVKEAQIALPISPVASAQSDPV
                     KERILALVVEKTGYPQDMLDLD"
BASE COUNT          909 a         1458 c         1477 g          880 t
ORIGIN      
        1 gggcgcatcg acgttcgtca agttctgcaa gatcggagcg ctgtccgcca ctggcactcg
       61 cccctacgcc gacggggcag acggatttgt catgggcgag ggcgccgcga tcttcctgct
      121 aaagtggctg gcagatgctg aacgcgacgg agacaagatc tacgctgtgc tgcgcggcat
      181 tggcgcttcc agcgacggca aggggaaagg aattacagcg ccgaacccca tcgggcaaaa
      241 actgtgcttt gagcgcgcat gggaaaacgc aggtctgcct gcggccaccg caaccttgat
      301 cgagggccac ggaacgtcca ctcgcgtggg cgacgtagtc gaagcgcaga gcctggccga
      361 agtgctgagc gttcatcatc ttccagcaca ttccgtggcg ctgggctcgg tcaaatcgaa
      421 catcgggcat ctcaaaggcg cggcgggcgc agccggcttg ctgaagaccg cactggcact
      481 gcgcgacaag ctcttgccgc ccagcgtaca ttgcgaacac cccagcgcgg atattgattt
      541 tgcgcactct cctctctatg tgaataccga actgaaaccc tgggctgcgc ccaaggactt
      601 tcctcgccgt gcaggagtga gcgcattcgg atttggcggg accaactttc acatggtgct
      661 ggaggagcat attcctcaca ggctgactgg aaacgggagg cgatccgtag ccgtcgcgac
      721 gattcctgaa actgcaatcc ccgaggtaac catgaaggct aaagaatcgc ccggcgcttc
      781 tgagtatgcc gcaatttcat ctcatgcacc ttgtcttgtc ggcgcccgca aggctccgtt
      841 gcggggggcc gtggtgatcg gggcagattc agaagccgca ctcgccgaac gcctgcgtac
      901 ggtatggaag gatgcgcagg cggggcgcac acccggtctc ggagcgcctg cagaagccga
      961 tttgcgtgct ccttaccgcc tggcaatcga ctacatcgac gcggccgatc tcgcagacaa
     1021 gtcttccaag gccctcaagg cccttactac gaaccagccc gctgtgtgga aggcgctgcg
     1081 cgcgcaaggc gtgtttcgcg gccagggtcc agcggcgaag gtcgcattcc tttatacggg
     1141 tcagggttcg cagtatgtga atatgctggc cggtctcggc gcggccgagc cgatcgttgg
     1201 cgataccttt gccgaagccg accgcgtgat gactccgcta ttaggcaagc cgctcagcaa
     1261 cttcatcttc gtggatccgt cgaacctgaa cgcagtcgcg aaggcagaag acgacctgcg
     1321 ccagaccgcc atcactcagc ccgctgtgct ggcagccgac ttggctctaa cccgcctgct
     1381 cgccgcctac ggcgtggagc cggacttcac catgggtcac agccttgggg aatacggggc
     1441 gctggtcgca tcaggcgctc ttcccttcgc tgatgctctt gaggcagtca gcgcccgtgg
     1501 gcgcgaaatg acgcgatttt ccctggctga caacgggcgg atggccgcgg tcttcgctcc
     1561 attgcctgag atcgaacgcc tgctgaagac gatcgacggc tatctggtga ttgcgaacat
     1621 caacagcgac catcaggcgg tgattggcgg cgcgagcaca gcggtcgagc gcgccatcga
     1681 ggtcttccag aaagccgggt acgaagttgc ggcgctgccg gtgagccatg ccttccacac
     1741 ttcgattgtc gcgcccgcaa gcgaaccgct gcgccgcacg ctggagcggc tgcacctgca
     1801 gtcgccgcgc gtgcccatcg tagccaacgt gaatggcgag ttctatcccc aggggccgga
     1861 tgtcgtgccg caaatgttgg acattctcgc caagcaggtt gcggctccgg tgcagttcgt
     1921 caaaggcctg cgcacgctct atgaggcggg cgttcgcgtc ttcgtggaag tgggaccgaa
     1981 gaaggccttg cagggattcg ccgaggatgt tctgggcgag cgcggcgacg tggtgtcgtt
     2041 cttcaccaat catccgaagt tcggcgatgt cccgtcattc aatcaggcgc tctgcggact
     2101 gtacgcggcc ggacttggcc gcgggacggg cgacgccgtg gctgcctccg ctcgcggtga
     2161 gacaaatgta ctaactcctg ccctgcccgc gtcgttcgag gcgccacgac aatctgctcg
     2221 actcgcctct gcccccggga tcgcgccagc tggagttaag gcttccgggg atcactttga
     2281 agaacttggc cgcttcttcg ccgatgttct cgagcgagga tggaagattc aaggtggccg
     2341 cgagcacgtt tcgagtaccg agcccgtcgt catcaccggc gcggctctgg gcctgccggg
     2401 aacccaacaa atttttgatg acgacaatct cgcacgcatt ctccgtgggg atcagttcat
     2461 tgatctcatt cctgcgcggt tccggcacgc catgctcgac aagcacatca atcggctggt
     2521 gaaaaccgac agtggggggc cgacgttcga aaccatcacc aatgtcgccg atgtgatcaa
     2581 gctggcgggc cgcgccggag catttgatct cgagaaggaa ttcggcgttc ccgccgagcg
     2641 catcgcagct cttgaccgcg tatctcagct ggctattgca gtgggcattg atgcattgcg
     2701 cgatgccggc attccgctgg tcatgcgtta taagaccacc agcaagggca cgaaactgcc
     2761 ggatcgctgg accctgcctg actcgctgcg cgacgacacc ggagtgatct tcgcctcggc
     2821 attcccgggc tacgactcct ttgccgacga aatgcgtcgt tactacaccg accactcccg
     2881 ccgcgaacaa ttgagcggac tggagagttt gatggcccgg gcgaagggca acggcgattc
     2941 gggcctcgaa caggaaatcg agcgccgcat tgaggatctg cgggatgcca tcgagaaaga
     3001 accctatgtg ttcgatcggc ggttcctgct tcgaacgctg gcgatggggc attcgcaatt
     3061 tgccgaattc atcggtgcac gtggtcctaa cacgcaaatc aacgctgcct gcgcgagcac
     3121 gacacaggcg gtaggcctgg ccgaagattg gatccgtgcc ggacgttgcc gtcgggtcat
     3181 cgtcatttct gcagacgaca tcacctccga ccacctgatg gactggtttg gcgcgggatt
     3241 cctcgcaagc ggcgcagcag ctaccgacga agtcgtggaa gaagccgcca ctccgttcga
     3301 ccggcggcgg cacggcatga tcgtaggcat gggcgccgcg gcgcttgtgg ttgagagcgc
     3361 acaggccgcg cgccaacgcg gactccagcc gatctgtgaa gttctcagcg ccgtaaccgc
     3421 caacagcgcc ttccatggca ctcgtctcga cgtgcagcat atcggcgagg tcatggaaga
     3481 actggtctcc cgggctgagt cccgcggaat ttcccggcat cagatcgcgc cgcacacggt
     3541 cttcgtctcg catgagactt acacgccggc gcgaggcggc agcgcttcgg ctgagatcaa
     3601 cgcgctgcga agagtattcg gcaaggcggc cgatcagatt gtcatcgcga acaccaaggg
     3661 ccttactggt catgccatgg caacagggat cgaagacgtg ctcgcggtca aaacactcga
     3721 aaccggggtg gtaccaccgg tcgccaactt caaggaggtc gatcccgagt tgggcacgtt
     3781 gaacttgtca aagggcgggg tgtatccggt cgaatacgcc ctgcggctcg gcgccggctt
     3841 tggctcgcag atcagcatga cactgctgcg ctggctggcc acgaaagacg gcgtgcaccg
     3901 ccgtcccaat gctctgggct atgcgtaccg catcgtggat gagaaatgct ggagggcgtg
     3961 gctcagccag accgcgggac gtccatcggc cgatctggaa gtggcgcatc gcacgctgcg
     4021 ggttcgggat cggcgaccgg caatggcggg ggttaccgaa cttgcgccac gggttgaagc
     4081 cgcgtctgcc gctagtcccg tgcagacgat caaaccgctt gccgaggtgc ctgcgacaca
     4141 tcgaccggcc gttctggccg tgtcaaaggt agacattgcg aacgttgtta ctgagccgcc
     4201 aacgccgata tcggcaccca tgtccccgtc cgtcgtcagg ccttcggctc ccccgctgca
     4261 gaagccggca gcggagggcg atccagtaaa ggagcgcatc ctcgctctcg ttgcagagaa
     4321 gacggggtac ccggtagaca tgctcgatct ggatctcgac ctcgaagctg atctgggcgt
     4381 ggatacggtg aaacaggccg aagtgttcgc cacgattcgc gaagcctaca gcatcgtgcg
     4441 cgatgacaag atcaggttgc gcgacttccc aaccctcgct catgtaatcc gcttcgtgca
     4501 tgagcggcgg tctgatctcg gccctgaacc ggccggattg cccgtggcca agccagaaac
     4561 cgcatttgca gccgctgtac cagagcctcc gctcgtaaaa gaggctcaaa tcgccctccc
     4621 catctcaccc gtggccagcg ctcaaagcga cccggtgaag gagcgcatcc tcgcgctggt
     4681 ggtcgagaaa acgggatacc cgcaggacat gctggatctg gacc
//
LOCUS       DASPUL010000061         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1105454, whole genome shotgun sequence.
ACCESSION   DASPUL010000061 DASPUL010000000
VERSION     DASPUL010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105454"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(453..537)
                     /locus_tag="VEJ00_01420"
     tRNA            complement(453..537)
                     /locus_tag="VEJ00_01420"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(501..503),aa:Leu,seq:tag)
     gene            complement(658..1206)
                     /locus_tag="VEJ00_01425"
     CDS             complement(658..1206)
                     /locus_tag="VEJ00_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99846.1"
                     /translation="MNTFALFGVSILGSFVSSVIAAWLFAWPRLKNTEPNRALTALVA
                     PHMFLRFIGLSFLVPGVVSPLLPAGFAIPAAYGDLIAGLLAIVATIALVNRKSWATRS
                     VWLFNIWGAADLLFAFYQARQVGIQPGMFGAAFFLVTAFVPPLLVTHGMIFRLLTQRP
                     VAATDDRNPRPERLTVQGQPVR"
     gene            1309..1779
                     /locus_tag="VEJ00_01430"
     CDS             1309..1779
                     /locus_tag="VEJ00_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXW99847.1"
                     /translation="MYIHTMKQPGVNYRDQIVVLPCACANLRRAARIVTQRYEHELRP
                     AGIKATQFTLLQALARAGNISQGKLGDLLGLDSTTLTRTLALLRSNGWIRAKPGDDRR
                     EVRLTLTPEGKRKYQSALHYWQSAQIQLRRSLGEAGWKQIMESTVRTAALAQQS"
     gene            complement(1844..2116)
                     /locus_tag="VEJ00_01435"
     CDS             complement(1844..2116)
                     /locus_tag="VEJ00_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99848.1"
                     /translation="MSLIEIVGSLFGIFAVVLIILLIYKSTLTMHEDDQLFLDDANSH
                     LQEEQTELLTKVNRLTIPVRVASIGSGIFLLAFLAMLIYQKLNEVQ"
     gene            2406..2768
                     /locus_tag="VEJ00_01440"
     CDS             2406..2768
                     /locus_tag="VEJ00_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HXW99849.1"
                     /translation="MATTTVPEVKKAAPITLTANAIAKVKEIMAQQNPVPAGLRIGVV
                     GGGCSGFSYSMQFENGAGMMDKTFDMEGLKVFVDATSVMYLNGCIVDYVETLEGAGFK
                     FENPNVKSTCGCGSSFSV"
     gene            complement(2982..>3714)
                     /locus_tag="VEJ00_01445"
     CDS             complement(2982..>3714)
                     /locus_tag="VEJ00_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99850.1"
                     /translation="TGGDFRLLHYGEVQMSTGGGIQGATAVSAMNGAPGSSGSAFGQS
                     STLGGSNTFGQSSFGQSSTFGGNSAFGNSNSTFGQNQPTNNQATGSEPSPAAPGNSDA
                     GNPEQNVAPVPGTSGNNDTTLASQQVQAVGGMPIVGVASLSKKETIREFNHKRKYNEW
                     QFIYDPETDRGGLLMTPNQPPLMPQAAPVNGQNGTNGTGSSFGNGVGNQPFGQSGFQN
                     NPQNNPVQPVTPQAPNDQNNPPPQQ"
BASE COUNT          799 a         1131 c         1070 g          714 t
ORIGIN      
        1 gctaggcgaa tgttgccaat gctcccaacc gtttatgtcg cgctgaaagc gaggcacgcg
       61 caagccggac accccgaaac gggttgggcg tttccttcgt cgtcgaggga aggtcacttt
      121 aacaaggaca cggccaagga ccagcacgca aaagccattc agcaagctaa agcgaaagcg
      181 aagaaggccg gtacgcgaga attggagcag ttccagccct acgtgctgcg acacacggcg
      241 ctcactcaac ttgcgcaggc cggatgcgac gtattcacac tagcgcgaat cgcagggcat
      301 agctccatca cgatcacaca gcgatatata catccgcagg cagacgcgat tgagcgagcc
      361 ttcgcgccgc ttgctgtcag tgagctagct caagtgggca caaatctagg cacagggaag
      421 aaagacgatc aagcgagagc actgcaaatg attggtgcga aaggggggac tcgaaccccc
      481 atgggtttcc ccgccagatc ctaagtctgg tgcgtctgcc aattccgcca ctttcgcacg
      541 gcgtaacccg attattgcac aagacccagc ccaggcccgg cagttgtcca acaatcgcgc
      601 ttaccctttc gcccgcccgc aaatctcgcg gttttgaggc ttttcagctg gcgttctcta
      661 tcgcactggc tggccctgga ccgtaagccg ctccggtcgc gggttgcgat cgtcggtcgc
      721 agcgactggc cgctgagtca gcaagcggaa gatcatcccg tgcgtgacca ggagcggcgg
      781 cacgaacgcg gtgacgagga aaaatgccgc gccgaacatt cccggttgaa tcccgacctg
      841 cctggcctga tagaaggcga acagcaagtc agcggctccc cagatgttga acagccacac
      901 gctccgggtg gcccacgatt tgcggttcac cagggcgatc gtggccacga tcgccaacag
      961 gccggcaatc aggtcgccgt acgccgcggg gatggcaaat ccagccggca gcaacggaga
     1021 aacgactccc ggcaccagga aactcaagcc gatgaagcgc aggaacatgt gaggcgccac
     1081 cagcgccgtc agcgcgcgat tcggttcggt gttcttaagc cgcggccacg caaagagcca
     1141 ggctgcgatc accgagctca caaagctccc cagaatgctg actccgaata gtgcgaaagt
     1201 gttcattagt tctcctcctt gcacttcagt tgttcgagcc gggactgtgc taatagatgt
     1261 atatgcacat aaagcggacg caaaaagttt gctgacggat gaaggtagat gtatatacat
     1321 actatgaaac agccgggagt gaactaccga gaccaaatcg ttgtcttgcc gtgcgcttgc
     1381 gcaaatctgc gccgcgctgc ccgtattgtg acccagcgat acgagcacga actgcggccg
     1441 gctgggataa aggcgacgca gtttacgctg ttgcaggctc tggcgcgcgc gggaaacatc
     1501 tcacagggca agttgggcga cctgctgggg ctggacagca cgaccctgac ccggacgctg
     1561 gcgctgttgc gaagcaacgg ctggatccgc gcgaaacctg gcgacgaccg gcgagaggtc
     1621 cgtttgactc tgaccccgga agggaaacgc aaataccaga gcgcgctgca ctattggcag
     1681 tcagctcaaa ttcaattgcg aagatcactc ggcgaagcag gctggaagca gattatggag
     1741 tcgacggtgc gcacagccgc gctggctcag caatcctgag cgtgcaccta tggcgaggcc
     1801 gcaccatcac cggcgatcga cgactctgcg tcggcaatgc cggctactgc acttcgttca
     1861 gcttctgata gatcaacatc gccaggaagg cgagcaggaa aattcccgaa ccaatgctgg
     1921 ccacgcgcac cggaatcgtc aggcgattga ccttggtcag cagctctgtc tgctcttctt
     1981 gcaggtgcga attcgcgtcg tccaggaaca gttgatcatc ttcgtgcatg gtcagcgtgc
     2041 tcttgtagat cagcaggatg atcaagacga ccgcgaagat cccgaacaga cttcccacga
     2101 tttcaatcag tgacataagc cacctcggca gattccatcg cagtgcagcg gggttccgct
     2161 accgctgcgg accatttcgg tccccacgcg gctcggggaa gaccctggcg gaatcgatta
     2221 tatccccgtt ttaaagatgc gccaccaaga aatccctgta ttcggaacct gttatgcacc
     2281 agggctcgat agagcccccg ccctctttgc cgcatctaat aaccgtgact ggcagtcccc
     2341 gaagccatgg ctccgggaaa tgacatctgc tataatcaaa cagctttaaa gccaaggaga
     2401 tacgaatggc cactacaacc gtcccggaag tcaagaaggc ggcgcccatt accctgaccg
     2461 cgaacgccat cgccaaagtc aaggagatca tggcccagca gaaccccgtt cccgccgggc
     2521 tccgcattgg agtcgtaggg ggcggatgct ctgggttctc ctattccatg cagtttgaaa
     2581 acggcgcggg catgatggac aagaccttcg acatggaggg cctcaaggtc ttcgtcgacg
     2641 ccacctcggt catgtatctc aacggctgca tcgtcgacta cgtcgaaact ctcgaaggcg
     2701 cgggcttcaa gttcgagaac ccgaacgtca agagcacctg cggctgcggc tcttccttca
     2761 gcgtgtagtc ggccatgtag ggacagccgc cttcggctgt ccgcgcgcgc agcgcggcac
     2821 gcagtctcag aaatgccaat ggcctcaccg tcgggtgggg ccattttcac ctcagagttt
     2881 tcaccacgga attcatcacc cagcaggctg tcattccaat gcgagcgaag caagcggagg
     2941 aggaacctgc ttcttaggca ccaaggaaga agacagcgag cctactgctg cggcggagga
     3001 ttgttctgat cgttgggcgc ttgcggcgtg acaggttgta cgggattgtt ctgcgggttg
     3061 ttctggaatc cgctctggcc aaaaggctgg tttccaactc cgttcccgaa gctgctgcca
     3121 gtaccgtttg tgccgttctg cccgttcacc ggtgctgcct gcggcatcag cggcggctgg
     3181 tttggcgtca tcagcagccc gccccggtcc gtttccggat cgtagatgaa ctgccactcg
     3241 ttgtactttc tcttgtgatt gaactcgcgg atcgtttcct tcttgctcag gctcgccact
     3301 ccaacgatcg gcatcccacc aacggcctgc acctgctggc tcgccaaggt ggtatcgttg
     3361 ttgccgctgg ttccggggac cggcgccacg ttctgctccg ggttgcccgc gtcactattg
     3421 cccggagccg ccggcgatgg ctccgatccc gtcgcctggt tattcgtggg ctgattctgc
     3481 ccgaacgtcg aattggaatt tccaaacgcg ctgttcccgc caaaagtcga cgattgcccg
     3541 aacgaggact gtccgaaggt gttgctcccg ccgagcgtag aggattgccc gaatgcagaa
     3601 ccgctcgatc ccggcgcacc gttcatcgcg ctgaccgccg ttgcgccctg aatcccgccg
     3661 ccagtcgaca tctgcacctc gccgtagtgc agcagccgga agtccccgcc tgta
//
LOCUS       DASPUL010000062         3655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1516815, whole genome shotgun sequence.
ACCESSION   DASPUL010000062 DASPUL010000000
VERSION     DASPUL010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1516815"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VEJ00_01450"
     CDS             complement(<1..1166)
                     /locus_tag="VEJ00_01450"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXW99851.1"
                     /translation="MPLKAHLLIVDDEANTLASLSRAFRLAGHEATVCDNAGKALELA
                     KAQNFDLILSDVVMPGKDGLTLLEELKAQGVTAQVVMMSGQAHIEMAVKATRLGALDF
                     LEKPISSDKLLLTVENALKLQRLENENRQLRQRLGKHEIVWKGEAMRRVMAQLERVAA
                     SESRVCILGETGTGKELVARTIHERSARAAGPFVTVNCAAVPAELIESELFGHEKGSF
                     TGASGRHIGKFEQADMGTIFLDEIGDMPLNMQAKLLRVLEEGEVERIGGDKPVAVNVR
                     VVVATHRDLEARVREEKFRQDLFHRVYVFPLTLPPLRERREDIPALIEHFAGQVCAQN
                     GWKPVPFTPEAMQTLQSHSWPGNVRELRNMVERLMLLATEGQIDMATVQLALPRA"
     gene            complement(1193..3046)
                     /locus_tag="VEJ00_01455"
     CDS             complement(1193..3046)
                     /locus_tag="VEJ00_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW99852.1"
                     /translation="MTFRRKLLAVFAFTVFVSVAAVAGLVLALTRRAFERMEDQQTGA
                     LMAQFQREFNRQGEDVVRRVEAISAAEPVIRMATALNGPSADSAGYFDLARSLAESQQ
                     LDFLEFLDGRGAIISSAQWPAKFGYPENGFESLSASTEEGAFLKQEELQDSTALGLFA
                     VRTTRAGDHPIYIIGGRRLDKNFLSTLELPTGMRALLYQNRGDRFSPDLLLDPLGAGN
                     TYHGSQSADRFATLIDAVRQYKQEMTGVVHWSSDQADDEVFHAIPLNGVGKDRPLLGI
                     LLIGNSRRPYVELKRHIRASALLVGGGGIVLAILLSSWAAARVTRPVEQLARAAQDVA
                     AGNWNTRVEVPGRDEVSQLADSFNRMTMELLTQKERLVQAERVAAWRELARRLAHELK
                     NPLFPLQLTVENLVRARLQNPDQFDEVFREGSRTLLAEISNLKTIIGRFSEFSKMPHP
                     KLQPVPINEMLRGIAQLFQAQLQAPGRAPISCKLELDEALQPVAADPDLLHRAVSNLV
                     LNAMDAMPNGGTLTLRSRRNAGKVILEVADTGSGLTPEECERIFTPYYTSKQHGTGLG
                     LAIVQSVVSDHKGRITVQSEPQHGTTFVIELPVNLETGEKLGAAGEVTSAG"
     gene            complement(3043..>3655)
                     /locus_tag="VEJ00_01460"
     CDS             complement(3043..>3655)
                     /locus_tag="VEJ00_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99853.1"
                     /translation="DGDAGSPDEKALREVLALSVERASIHNVVFQGSGQPAGGLLPDW
                     MSGYGFVFPTEPDLKRARHDREAVHNAPAWTLGYDDSDPLARLIAERVALNAHDAGLA
                     VQPTATAKTGLRLVRIPLVSADPWVALAEAEGAAGLPAAKSKGGSAEDLYAAEQAELA
                     TQRIIPLLHLPVSYAAASSVKNWSLRPDGSWNLGDAWLGSGKP"
BASE COUNT          693 a         1193 c         1014 g          755 t
ORIGIN      
        1 gctctgggca aagccagctg cacggtcgcc atatcaatct gaccctcggt cgcgagcagc
       61 atcagccgct cgaccatgtt gcgcagctcg cgcacgttcc ccggccagga gtgcgattgc
      121 agcgtctgca tcgcttccgg agtgaatgga accggcttcc agccgttctg tgcgcagact
      181 tgtccggcga agtgctcgat cagggctgga atatcttcac gcctctcgcg cagaggcggc
      241 agagtcaggg ggaaaacgta gacgcgatga aacaggtctt gccggaattt ttcctcgcgg
      301 acgcgagctt ccagatcccg gtgtgtcgcc acaaccaccc ggacattcac ggcaaccggc
      361 ttgtcccctc caatgcgctc cacttctcct tcttcaagca cgcgcaacag cttggcctgc
      421 atgttcagtg gcatgtctcc gatctcgtcg agaaagatcg ttcccatatc ggcctgttcg
      481 aactttccga tgtggcggcc ggaggcgccc gtgaacgagc ccttctcgtg tccgaacagt
      541 tccgattcaa tcagttctgc cggcacggcc gcgcagttca ccgtcacgaa agggccggct
      601 gcccgcgcac tccgctcgtg aatcgtacgc gccaccagtt ctttgcctgt accggtctcg
      661 cccaaaatgc acacccgcga ttcgctggcc gcaacccgct ccagttgcgc catgacacgg
      721 cgcatggcct cacctttcca cacaatttcg tgtttcccta accgctggcg gagttgccgg
      781 ttttcgtttt ccagccgctg taacttgaga gcgttctcca ccgtgagcaa cagtttgtcg
      841 ctggaaatcg gtttttccag aaagtcaagc gccccgagcc gagtggcctt caccgccatt
      901 tcgatgtgtg cctgccctga catcatgacg acctgcgcgg tcactccctg cgctttcagt
      961 tcctcgagca aggtgaggcc gtctttgccg ggcatgacga cgtccgagag aatcaggtcg
     1021 aagttctgtg ctttggccag ttccagcgct ttgccggcgt tgtcacagac ggtggcttcg
     1081 tgtcctgcaa gacggaaagc acgcgacaat gacgccagtg tgttggcttc atcatcaacg
     1141 atcaaaagat gggctttcag tggcaaccct cctcctcatt cgcaactgga gatcagccgg
     1201 cgcttgtgac ttcgccagca gcaccgagtt tctcacctgt ttccaaattc acgggtaatt
     1261 cgattacaaa agtcgtccca tgttgaggtt cactctgaac cgtgattctt cccttatggt
     1321 cactgactac ggactgcaca atcgcgaggc cgagaccggt cccatgctgt ttgctggtgt
     1381 agtacggagt aaagatgcgc tcgcactctt ccggcgtcag cccggagccg gtgtcggcga
     1441 cttcaaggat cactttccca gcgttgcggc ggctgcgcaa tgtcagcgta ccgccgttgg
     1501 gcattgcgtc catcgcgtta agcaccagat tcgagacggc gcggtgcagc agatcgggat
     1561 cggcggcgac tggctgaagg gcctcatcaa gctcaagctt gcagctgatc ggtgcccggc
     1621 ctggcgcttg cagttgcgcc tgaaagagct gtgcaatccc gcgcagcatt tcattgatcg
     1681 gaaccggctg caacttggga tgcggcatct tcgagaattc gctgaaacgc ccgataatcg
     1741 ttttcagatt cgagatctcg gccagcagcg tgcgcgagcc ctcgcggaac acttcgtcga
     1801 actggtcggg attctgcagc cgtgcccgca ccagattttc gaccgtaagt tgcagcggga
     1861 acagcggatt cttcagctca tgcgccagcc gccgcgccag ttctcgccag gccgcgactc
     1921 gctccgcctg cactaggcgt tctttctggg tcagcaattc catcgtcatg cggttgaacg
     1981 aatcggcgag ctgcgagacc tcgtctcggc cgggcacctc aacccgtgtg ttccagttgc
     2041 cggccgcgac atcctgtgct gcccgggcca gctgctcgac tggccgtgtc accctggctg
     2101 cagcccagct gctgagcaag atcgccagaa cgatcccgcc gccgcccacc agcaatgccg
     2161 acgcacgaat atgccgcttc agctccacgt acgggcgccg tgagtttccg atgagcagaa
     2221 tccccagcaa aggacgatct ttccccacac cattcagggg aatcgcatga aacacctcgt
     2281 catccgcctg atctgacgac caatgcacaa cccccgtcat ctcctgcttg tattgccgaa
     2341 ccgcatcgat cagtgtggca aaccggtcgg cagactgcga accatggtac gtattcccgg
     2401 cccctaacgg gtccagcagc agatccggcg agaaacgatc ccctcgattc tgatacaaca
     2461 aggctcgcat tcctgtaggc agctcaaggg tcgagaggaa attcttgtcg agccggcgtc
     2521 ctccgatgat gtaaatcgga tggtcgcccg cgcgagtagt tcgcactgcg aacaggccga
     2581 gcgccgttga atcctgcagt tcctcctgct tcaggaatgc tccctcctcg gtcgatgctg
     2641 aaagactctc gaagccgttt tcgggatatc caaacttggc cggccactgc gcggaagaaa
     2701 tgatcgcgcc gcgcccgtct agaaattcga gaaagtcgag ctgctggctc tctgccaatg
     2761 atctggcaag atcgaaatat ccggcagagt ctgccgacgg gccgttcagc gctgtcgcca
     2821 tccggatgac cggttccgcc gcggaaatcg cctcgactcg ccgcactacg tcttcaccct
     2881 gacggttaaa ctcgcgctgg aattgtgcca tgagcgcgcc cgtctgctga tcttccattc
     2941 gctcgaatgc ccgccgagtc agcgccaaaa ccaatccggc aaccgccgcc actgatacga
     3001 aaacggtgaa tgcaaagacg gccagcagtt tgcggcggaa ggtcacggct ttccgctccc
     3061 cagccacgcg tcgccgagat tccagcttcc gtcgggcctt agagaccagt tcttgacgga
     3121 gctagccgcg gcataggaca caggcagatg aaggagcgga ataattctct gcgtcgcgag
     3181 ttcggcttgt tctgctgcgt aaaggtcctc ggcagacccg cccttgcttt ttgccgcagg
     3241 caggccggcc gctccctcag cctccgccaa agcgacccac ggatcggccg ataccagcgg
     3301 gatgcgcacc agccgcaggc cggtcttggc cgtcgcggtc ggctgcacgg ccaatccagc
     3361 gtcgtgcgcg ttcagcgcta cgcgttcggc gatcagtcgc gccagcgggt cgctgtcgtc
     3421 gtatcccaac gtccacgccg gggcattatg cactgcctcg cgatcgtggc gcgcacgctt
     3481 caaatccggt tccgtgggaa acacgaatcc gtagccactc atccagtccg gcagaaggcc
     3541 gcctgcaggt tgtccgctgc cttggaacac tacattgtga atcgaggccc gctcaacgct
     3601 cagcgccaac acctctcgta gcgctttctc atccggcgac cccgcgtcgc catca
//
LOCUS       DASPUL010000063         6762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_831900, whole genome shotgun sequence.
ACCESSION   DASPUL010000063 DASPUL010000000
VERSION     DASPUL010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831900"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VEJ00_01465"
     CDS             complement(<1..483)
                     /locus_tag="VEJ00_01465"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HXW99854.1"
                     /translation="MRKLLFIAVLIPVLGGIAWWYLREQGLPDYGPAYREYAYVANGK
                     SNTVSVIDLRTFALAKTIQVGSEPTGIAANSRKNEIYVVNSGSNNVSVIDAETNSVVT
                     TIGVHGRPYFIDVSEDGKRAYVANSGSSNISVIDLEKRLVLGNVRVGAAPGLARVSPD
                     G"
     gene            complement(567..974)
                     /locus_tag="VEJ00_01470"
     CDS             complement(567..974)
                     /locus_tag="VEJ00_01470"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXW99855.1"
                     /translation="MSSICVRLLVVLGATLVVVSSTHLLAQDSSPRNGEVIYEFHKAG
                     ENGITAPKGIFMPQPEYTDQARRKKINGTVLLSLVVNADGTVRDPAVTKSLDKGLDKQ
                     ALETVKKWKFQPATKDDQPVAVRIDVEVSFRIR"
     gene            complement(1017..2075)
                     /gene="aroF"
                     /locus_tag="VEJ00_01475"
     CDS             complement(1017..2075)
                     /gene="aroF"
                     /locus_tag="VEJ00_01475"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXW99856.1"
                     /translation="MLVVMKAHATEEQVRDVCQKIEKLGYRAHPMPGATRTAIGITGN
                     KGEVEQGTLEEMPGVQEVIAVSKPYKLVSRDIKEENTVIRFQGTNATMGGPALAIVAG
                     PCAIESREQAFTVAERVYRAGAQFFRGGAYKPRTSPYSFQGLGEDGLRIMAEIRQQFG
                     MKIVTEAIDHESLDLVEEYADVIQIGARNMQNFSLLKRAGRARKPVLLKRGMSATLEE
                     FLMAAEYILSEGNYNVALCERGVRTFADHTRNTLDLSIVPAIQRLSHLPILVDPSHGT
                     GKRNKVTPLSRAAVAVGADGLLVEVHHKPDEALSDGPQSLFPDQFDELMVQVRQIAAV
                     VGRTVPAIGVPEAVAVSK"
     gene            2296..3282
                     /locus_tag="VEJ00_01480"
     CDS             2296..3282
                     /locus_tag="VEJ00_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXW99857.1"
                     /translation="MKAAVVPSVNGKWEVKEWQTPKAGPNQVVIKIHASGLCYTDVHI
                     THGVIPTKFPRVLGHEPVGEIVEAGVGVTSRKVGDRVGVGWHQKGCGRCEWCLRGKKE
                     LCPYAICTGIGLEGSHGEYMLAYADATMLIPDGLSYEQAAPIFCAGYTVWSGLRLADP
                     KPHERIAVVGIGGLGHLALQYSKAAGFETIAVTQSKDKEKMIRDMGADEVVANGEKLL
                     ASGGADVLLATSNSWAATAEAAKGMRQYGRVILMGVGPEPLNYPPELMFRHVAVIGSS
                     QNGPEYLYEALDYAAKGKVKVVAETYKLDDIGQAYDRVADGKVRFRAVITMN"
     gene            3338..4258
                     /locus_tag="VEJ00_01485"
     CDS             3338..4258
                     /locus_tag="VEJ00_01485"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HXW99858.1"
                     /translation="MPRSIAQEQDIFKRPPRRPPRLTARRIGIHTSTAGGPHNAAERA
                     YRLGCNTLQIFSSSPRQWAPFELSGPQCDEMSRLRARYDLKPLVIHTNYLVNLASSNE
                     LFLQKSMEAFRGELERALTLGAEYLVLHPGSFRGADREQGLLRTAAAIAAATRGLDLV
                     QGGLTILIENTAGSEFSLGGSFEQVSEILERLRGIVPVGACIDTCHTHVAGYDIATEG
                     GLYQTLQHLEATIGLNNVKVWHCNDAKAPLGSKLDRHQHIGKGMIGKETFRILLNDLR
                     LGHAAFIAETPIDKPGDDRRNVDALKRLVA"
     gene            4346..>6762
                     /gene="leuS"
                     /locus_tag="VEJ00_01490"
     CDS             4346..>6762
                     /gene="leuS"
                     /locus_tag="VEJ00_01490"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXW99859.1"
                     /translation="MSPEQKSQATMTRADRPKNDRPDSRENDERYDAQRVEMKWSERW
                     LQDAELYAAERDSAKKKYYVLEMLPYPSGALHMGHVRNYSIGDALARYMWMNGYNVLH
                     PMGWDSFGLPAENAAISANVPPREWTLRNIANMKTQMKRIGFAYDWSREVTTCFPEYY
                     RWNQWFFLKLYEHGLAYRKKSKVNWCPKCATVLANEQVVNGCCWRHEDTPVEQRELEQ
                     WFLRITKYADELLRDLDKLSGWPEKVRTMQRNWIGRSEGTLVDFKLDGTAGPAGSTIT
                     VFTTRVDTIFGATSVQLAPEHPLVADFTRYNQPLGEAVQQLIAEQRKAKEEGDIGLIE
                     KHGVNTHHYAINPYNGEKVPIWIANYILMDYGTGAIMSVPAHDERDYEFAKKYKLEIR
                     LVILPVSDDPEETITEPALPFTTLYGSLVNSGRFSGLMCEDAIKKMSVYAEENKFGKA
                     TVTYRIKDWGISRQRYWGTPIPMLYCEKDGIVPVPEKDLPVILPEKVEITLTGGSPLE
                     SVPAFVRATCPKCGGPARRETDTMDTFVDSSWYFYRYTDARNDNAPFDGKVAQYWFGD
                     CGIDQYIGGVEHAILHLIYSRFWTKFMRDLGLITNDEPVERLFTQGMVIKEGRKMSKN
                     LGNVVSPDEMVARYGADAARMYSLFAAPPDRDLDWQDSGIEGIQRFLGRVYRFVVRNS
                     QPEHPDWRKPVPADLLPQARTIQRKLHQTIKRISDDFQGRWHFNTCISAVMELVNVLY
                     GAEEAIAQNAVPTPFLAEVQRKLVLLLAPFAPYLAHELWEMLGEKEGLLRAPWPRYDA
                     ELAKEEE"
BASE COUNT         1386 a         1998 c         2039 g         1339 t
ORIGIN      
        1 tccatcgggc gaaacccgtg ccaggccggg cgctgcgccg acgcgaacgt tcccaagtac
       61 cagcctcttc tcgagatcaa tcacgctgat gttcgatgac cctgaattgg ccacgtaggc
      121 gcgtttgccg tcttcagaga cgtcgatgaa atagggccga ccgtgtacgc cgattgtcgt
      181 tacgactgag ttcgtctcgg cgtcgatcac gctgacgttg ttcgatccgc tgttgactac
      241 atagatttcg ttcttcctgc tgtttgcggc aatgccggtc ggttcgctgc cgacctggat
      301 cgtcttcgcc agtgcaaacg tgcgtaagtc aatcacgctg acggtattgc tcttgccatt
      361 ggcgacgtaa gcatattcac ggtaggccgg gccgtagtcg ggcagtccct gctcgcggag
      421 ataccaccag gcaattccgc ccaacacggg gatcaacact gcgatgaaga ggagctttct
      481 caaagttgcg cgtctgccgg agaagatgcc gaggagacaa agactagcag aaattgaaag
      541 cgatgaccgt gacgccggga agcaacctat cgaatcctga atgaaacttc tacgtcgatg
      601 cgcaccgcca caggttggtc gtctttggtg gccggctgga acttccactt cttcaccgtt
      661 tccagcgcct gcttgtcgag ccctttgtca aggcttttgg tcactgcggg atcacgcact
      721 gttccgtcgg cattcacgac caacgagagc agcacggtcc cattgatttt ctttcgccgc
      781 gcttggtcgg tgtattcggg ttggggcatg aagatgccct tgggggctgt gataccgttc
      841 tcgcccgctt tgtggaactc gtaaatgacc tcaccgtttc ttggcgagga gtcttgcgcg
      901 agcaggtggg tgctcgaaac cactacaagc gtggcgccca gcacaaccaa aaggcggaca
      961 catatgctcg acataagttc tccccaactg cagagtttgg gtcccccaaa gcgagcctac
     1021 ttgctcactg caacggcttc cggcacgccg atcgccggca cagtccttcc caccacggct
     1081 gcaatctgac ggacctgcac catcaattca tcgaactgat ccgggaacag cgactgcgga
     1141 ccgtctgaaa gtgcctcatc gggcttgtga tgtacttcca cgagtaagcc gtcggcaccc
     1201 actgcaacgg ccgcgcgcga cagcggcgtg actttgttgc gcttgccggt gccgtggctg
     1261 ggatcgacga ggatcggcaa gtggctgagt cgctgaatcg caggcacgat gctgaggtcg
     1321 agcgtgttgc gggtatggtc ggcaaacgta cgcacgccgc gctcacacag cgccacgttg
     1381 tagtttccct cgctcaggat gtattcggcg gccatcagaa attcttcgag cgtggccgac
     1441 atgccgcgct tcagcagcac cggcttgcgg gcgcgtcccg cgcgcttaag cagggaaaaa
     1501 ttctgcatgt tgcgggcgcc gatctggatc acgtcggcgt actcttccac cagatcgagc
     1561 gactcgtgat cgatggcctc ggtcacaatc ttcatgccga attgctggcg gatctctgcc
     1621 atgatgcgca ggccgtcctc gccaaggccc tgaaacgaat atggagacgt gcgcggcttg
     1681 taagcgccgc cacggaaaaa ctgcgctccg gcgcgataga ctcgctcagc cacggtaaag
     1741 gcctgctcac ggctctcgat ggcgcacggc ccggcaacaa tcgcaagggc cggcccgccc
     1801 atcgtggcgt tcgtgccttg aaaacgaata accgtgtttt cttccttgat gtcgcggctg
     1861 accagtttgt agggcttcga gacggcgatg acttcctgca cgcccggcat ttcttccagc
     1921 gtgccctgtt cgacttcgcc cttgttgccg gtaatgccaa tcgcggtgcg cgtcgcgccc
     1981 ggcatgggat gcgcgcgata acccagcttc tcgattttct ggcagacgtc gcgtacctgc
     2041 tcttcggtgg cgtgcgcctt catgacgact aacataggtt caccttgggg taaattgtct
     2101 gagacagcca tcccggctgt cgaatcgagt gacgctcgat tggcccactg cggcgcagga
     2161 gaaaccgccc gtcaagtcta ccgttgccgc gccctgcacc gcaaacgggg cattgtcccg
     2221 tgaatccacg agactcaggt agcatccaaa gggcattcgt gaccggcgaa tccgaattct
     2281 tccaggagcg ttctcatgaa agctgcagtg gttccctccg ttaacggcaa atgggaagtc
     2341 aaggagtggc agacacccaa ggcagggcca aaccaggtcg tcatcaagat tcacgccagc
     2401 ggcctgtgct acaccgacgt gcacatcacg cacggcgtca tccctacgaa atttccacgc
     2461 gtgctcggtc acgagcctgt gggagagatc gtggaggctg gtgtgggcgt gacctcccgc
     2521 aaggtcggag accgtgtggg cgtgggctgg caccagaagg gctgcggtcg ctgcgagtgg
     2581 tgcctgcgcg gcaagaagga actttgcccc tacgccatct gcaccggcat tggcctcgaa
     2641 ggcagccacg gcgagtacat gctcgcctac gccgacgcga ccatgttgat tcccgacggg
     2701 ctcagctatg aacaggcggc gccgatcttt tgcgccggct acacggtgtg gagcgggtta
     2761 cggctggccg atcccaagcc acatgagcgt atcgctgtcg tgggaatcgg cggacttggt
     2821 cacctggcgc tgcaatattc gaaggctgcc gggtttgaga ccatcgcggt cacgcagtcc
     2881 aaggacaagg aaaagatgat ccgcgacatg ggtgcggatg aggtcgtcgc gaacggcgaa
     2941 aagctgctgg cttcaggagg cgccgatgtg ctgctggcga cgtcgaattc gtgggctgca
     3001 actgccgagg ccgccaaggg catgcgccag tatggacgtg tcattctcat gggcgtcggt
     3061 cccgagccgc tgaactatcc tccggagctg atgttccggc atgtggcggt aattggttcg
     3121 agccagaacg gccccgagta tctctacgag gctctcgact atgccgccaa aggcaaggtt
     3181 aaagttgtgg ccgaaaccta caagctggat gacatcggcc aggcgtatga ccgtgtcgca
     3241 gatggaaagg tgcggttccg ggctgtgatc acgatgaact gaagccggct cgaactgcag
     3301 ccaaaggacc ggtccggacg cctttatact gggcagcatg ccgcgttcca tcgcgcagga
     3361 gcaggacatt ttcaagcggc cgccgagacg tccgccgcgc ctgactgcgc gccgcatcgg
     3421 cattcacacc tcgactgccg gaggaccgca caacgcagcc gaacgcgcct accgcctggg
     3481 ctgcaataca ttgcagattt tttcttccag cccgcgacag tgggcaccgt ttgaactcag
     3541 tggtccgcag tgcgacgaaa tgagccgcct gcgcgccagg tatgatctga agccgctcgt
     3601 cattcacacg aattatctgg tgaacctcgc cagctcgaat gagctgtttc tgcaaaagtc
     3661 gatggaagca ttccgcggcg agctggaacg tgcgctcacg ctgggcgccg aatacctggt
     3721 gcttcatccc gggtcgtttc gcggtgccga ccgagagcag ggattgctcc gtaccgccgc
     3781 ggccatcgca gcggcaacgc gcggccttga tctcgttcag ggcggtctga cgattctgat
     3841 cgagaacacg gcggggtcag agttctccct tgggggcagc tttgaacagg tttccgagat
     3901 cctggaacgc ctgcgcggga tagtccccgt cggcgcctgc atcgacacct gccacacgca
     3961 cgttgcggga tacgacatcg ctacggaagg cggcctttac cagacgctgc agcatcttga
     4021 ggctaccatt ggcctgaaca acgtcaaagt ctggcactgc aatgacgcca aagccccgct
     4081 tggatcgaag ctcgaccgtc atcagcacat cgggaaggga atgatcggca aggagacatt
     4141 tcgcattttg ttgaacgacc tgcggctggg ccatgcagca ttcatcgcgg aaacgccgat
     4201 cgacaaaccg ggagatgatc gcaggaatgt agatgcgttg aagcggctgg ttgcctgatc
     4261 cgccggagaa tcgagttgcc gaaggctaca tttcccacac tccgttgtag aatgaacgat
     4321 tatcctgtag cggttgctcg cgcctatgtc gcccgaacaa aagtcgcaag ccacgatgac
     4381 ccgcgctgat cgcccaaaga acgatcgtcc tgattctcgc gagaatgacg agcgctacga
     4441 cgcgcagcgc gtcgagatga agtggtcgga gcgctggctg caggacgctg agctctacgc
     4501 cgcagagcgc gactccgcca aaaagaagta ttacgtcctg gagatgctgc cctatccctc
     4561 gggcgctctg cacatggggc atgtgcgcaa ctactccatc ggcgacgcgc tggcccgcta
     4621 catgtggatg aacggctata acgttctcca tcccatgggg tgggattcct tcggcttgcc
     4681 ggccgaaaat gcggccatct ccgctaatgt accgccccgg gagtggacgc tccgcaacat
     4741 cgcgaacatg aagacgcaga tgaagcggat cggcttcgcc tacgactggt cacgtgaagt
     4801 tactacgtgc ttcccggagt actaccgctg gaaccagtgg ttcttcctca agctctacga
     4861 gcacggcttg gcgtatcgca agaagagcaa agtcaactgg tgcccgaagt gcgcgaccgt
     4921 gctggcgaat gagcaggtgg taaacggctg ctgctggcgc cacgaagaca cgcccgtcga
     4981 gcaacgtgaa ctcgagcaat ggttcctgcg catcacgaag tatgccgatg agttgttgcg
     5041 cgatctcgac aagctcagcg gctggccgga gaaagtgcgc accatgcagc gcaactggat
     5101 cggccgcagc gaaggaacgc tggtcgattt caagctcgac ggtacggccg gtccggcagg
     5161 ttcgaccatc acggtattca ctacgcgtgt ggatacgatt tttggcgcga cctcggtgca
     5221 gcttgcgccc gagcacccgc tggtcgccga cttcacgcgc tacaaccagc ccctgggcga
     5281 agccgtgcag cagttgatcg ccgagcagcg caaggccaaa gaagagggcg acatcggcct
     5341 gatcgagaag cacggcgtca acacgcacca ttacgccatt aatccttaca acggcgagaa
     5401 ggtgccgatc tggatcgcga actacatcct gatggactac ggcaccggag ccatcatgag
     5461 cgtgcccgcg cacgacgagc gcgattacga gtttgcgaag aaatacaagc tcgaaatcag
     5521 acttgtgatt cttcctgtga gcgatgaccc ggaagaaacc attaccgagc cggcgcttcc
     5581 gttcaccacc ctctacggct cgctggtcaa ctcggggcgc ttcagcggcc tgatgtgcga
     5641 agacgccatc aagaagatgt ccgtgtacgc cgaagagaac aagttcggca aggcgacggt
     5701 tacttatcgc atcaaggatt ggggtatctc gcgccagcgt tattggggta cgccgatccc
     5761 gatgctttac tgcgaaaagg acggcatcgt acccgtgccg gaaaaagact tgccggtgat
     5821 cttgccggag aaggtcgaga tcacgctgac gggcggctcg ccacttgaga gcgtgccggc
     5881 gttcgtcagg gcgacctgtc cgaagtgcgg cggccctgcg cggcgcgaga ccgacaccat
     5941 ggacacattc gtggattcgt cctggtattt ttatcgctat accgacgcgc gcaacgacaa
     6001 cgcgcctttc gatgggaaag tcgctcagta ctggttcggc gattgcggca ttgatcaata
     6061 tatcggcggt gtcgagcacg cgatcttgca tttgatctac tcgcgcttct ggacgaaatt
     6121 catgcgcgat ctcggcctta tcaccaacga cgaaccggtc gaacgcctgt tcacccaggg
     6181 catggtgatc aaagagggtc gtaagatgtc caagaacttg ggaaacgtgg tgtcgcccga
     6241 cgaaatggtc gcgcgctacg gggccgacgc cgcccgcatg tacagccttt tcgctgcgcc
     6301 acccgatcgc gatctcgact ggcaggattc ggggattgaa ggcattcaga gattcctcgg
     6361 gcgtgtttat cgcttcgtgg tacgcaattc gcagccggag catcccgact ggcgcaagcc
     6421 cgtgccagcc gatttactgc cccaggcgcg caccatccag cgcaagctgc accagacgat
     6481 caaacgcatc agcgacgact ttcagggccg gtggcatttc aatacctgca tctcggcggt
     6541 catggagttg gtaaacgttc tgtacggcgc ggaagaggcc attgcgcaga atgcggtgcc
     6601 gacgccgttc ctggccgagg tgcagcgcaa gcttgtgttg ttgcttgcgc cgtttgcgcc
     6661 ctatttggcc cacgaactct gggaaatgct gggcgaaaag gaaggcttgc ttcgcgcacc
     6721 ctggccaagg tacgacgcgg agctggcgaa agaagaagag at
//
LOCUS       DASPUL010000064         7491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_420468, whole genome shotgun sequence.
ACCESSION   DASPUL010000064 DASPUL010000000
VERSION     DASPUL010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420468"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="VEJ00_01495"
     CDS             complement(<1..1069)
                     /locus_tag="VEJ00_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99860.1"
                     /translation="MAKSPILVSNGPPLRTFNAVAAAKILGQLRISFVHGCLALMLFA
                     SGIFSLARAQQWDDCSPSSEVKAGLDALPEPSHELTEWEFHRKELEAVQALRRQHPDN
                     VFIERRYMKSMHDRVDRPGVIEEYKARYIASPGSPLAAYLYGVVLFGRDSPQSIKLLD
                     SALEKDPQFPWPHIPLFWIYSAPVFRDRSKAEEHVRAFLAQCPASFEGYEQLARSDED
                     QAAVGKRAAQLRALIEKREDIDAIAAYRTLWTLEFKATPSSEYAGQRAQTARDIQRIR
                     ALNMQSKRQWYDVLEEGYKLTNDEKNATWAKEERASRDPSSWAPPSFIKWFEDHPYPA
                     EDAPGEIRQTYNKDLLAQSSTW"
     gene            1250..2872
                     /locus_tag="VEJ00_01500"
     CDS             1250..2872
                     /locus_tag="VEJ00_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99861.1"
                     /translation="MKKAACLLLLSVFFFTLAAFADDDHDMHHHEALTETQLGTVHFP
                     VSCAESVQKSFARGVALLHSFWYEEAEKEFLDISKDDPHCAMAHWGVAMSLWHQLWNE
                     PDAKVIARGLSEAKAGEKLARKATPREQAYLAAIAAFYSHSDKLDHAARAKAYSEAMR
                     KVYESYPDDHEAATFYALSLLASEPHNDETFANRKQAAAILEKLFAIEPDHPGVAHYL
                     IHSYDKPQLAQLGIPAARRYAQIAPAAPHALHMPSHIFARVGLWQDDINSNLASVAAT
                     RKTAAMGMGGEGHQFHAMDFLVYAYLQSGREAEAAKIIDEVKTMPPMHDMYGMGFDPR
                     TYALSEFPAIYDLELRHWSDAAALVPVEGAHGSRSVTFWARAIGAARSGNVVQAKQDL
                     EQIEAVRKQMLADKNTDFAEAAEQDYQEASAWIAHAEGKDDEAIATLRTVADKNDKLG
                     DEPRTIPAREMLADLLLETKRPQQALTEYQTDLKINPNRFNGLYGAARAAEEAGKQSD
                     ANEYYALLLKTCEGGSSTRPELSRAKELVAQK"
     gene            complement(2875..3438)
                     /locus_tag="VEJ00_01505"
     CDS             complement(2875..3438)
                     /locus_tag="VEJ00_01505"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXW99862.1"
                     /translation="MAEWSRAKWAKIARRIRVPLGFAFAVFYFWLARPTWRFLAFGAV
                     LIVPGLLIRALASGHVRKNEALATSGPYAYTRNPLYLGSLLIGMGFAVAARSWWIGGA
                     LVVMLVVIYLPVIRDEEAFLREKFPEFEVYARQVPRMLPRIVPARIGGEGGGFSMALY
                     LKHREWNALLGSALLTGALIAKMTWLS"
     gene            complement(3431..4561)
                     /locus_tag="VEJ00_01510"
     CDS             complement(3431..4561)
                     /locus_tag="VEJ00_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXW99863.1"
                     /translation="MSANSDDGGVVERLLIVRLSAMGDVIHTLPAAQALRSAFPRAMI
                     GWLIEERWAELLCAPGTPRRGPRSARRPLVDWVHTVNLRKWRSSLAGLPTLQEIAKTW
                     NDVRSARYDVAVDLQGAIRSAVLARWSGARAVYGAAEARESPASLWYTSRAGGGGAHV
                     IEQNLAVVEAVAKKKLTVQRVELPHDAEAERRIDQQLAELGVRDFIILSPGAGWGAKR
                     WPVERYGQVARGLAADGIQSFLNYGPGEEALARDAEAASGGAARALECSVSELIALTR
                     RARLFVGGDTGPMHLAAALQVPVVAIFGPTDPTRNGPYATRSIVLRNPASPTTHARRA
                     QPDEGLLEISVSAVVEAARKLLSSSGHEGESTHATLKDGAHG"
     gene            complement(4558..5058)
                     /locus_tag="VEJ00_01515"
     CDS             complement(4558..5058)
                     /locus_tag="VEJ00_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HXW99864.1"
                     /translation="MRTPGQSHDKILTREELRRRVEEWRGAGERVTLANGGFDLLHVG
                     HVRYLRGAKALGGKLVVAVNSDESVRALKGEGRPIMPAEERAEIVAALADVDAVVIFS
                     ELDVRPLIREIRPDIHAKGTDYTEESVPERDAVAEYGGRVAIVGDPKNHSTSEIIRAQ
                     RSPRKS"
     gene            complement(5058..6137)
                     /locus_tag="VEJ00_01520"
     CDS             complement(5058..6137)
                     /locus_tag="VEJ00_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXW99865.1"
                     /translation="MRKSARSFGAATPLNMTKHPKEGERLRKIVEAFPNVTVTVLGDL
                     VADEFIFGEISRVSREAPVLILKHRERTVVPGGGANAANNLADLGVNVLPVGIVGDDE
                     PGRLLLKYFRHKRIAVSGVLKDKSYSTITKTRILAGMTHTARQQVVRVDREPHEAPNA
                     HLTRELYLAARNYAHASDALLVSDYGYGAATPALVDALQQKGKLGALPLVLDSRYRLL
                     EYSGITAATPNEPEVEEALGLRIGQDWAKLLAAGEQLMARMKLQSLVITRGRDGMAAF
                     DHKHKPVDIPIYGSDQVADVTGAGDTVIAAFTAALAAGATTEEAAQLANYAGGIVVMK
                     RGTATVSMDELLHALEQAPPATRPH"
     gene            complement(6134..7078)
                     /gene="lpxK"
                     /locus_tag="VEJ00_01525"
     CDS             complement(6134..7078)
                     /gene="lpxK"
                     /locus_tag="VEJ00_01525"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HXW99866.1"
                     /translation="MNPLSTIYGGMVGARNALYDRGFSHAHRLQGPVVSVGNLSAGGS
                     GKTPFVMLLGELLKARGVRFDVLSRGYGRKSRGALLVDPGGLPQHFGDEPLLIARKLR
                     VSVIVGEERYEAGRLAESKFGPQMHLLDDGFQHRMLARDFDIVLVTPQDASDQLLPAG
                     RLREPLASLSRADSIVLASGATAESFPLVGKLVWRVRRGIATQNAPPRPVVFCGIARP
                     QTFVLQLRAAQIEPAAEAFYRDHHAYSEKDVRELLALKQKSEAGGFVTTEKDAINLGP
                     YLSALEPLSVIPVRMELADATNALDTMLRVIEERKRRA"
     gene            complement(7075..>7491)
                     /locus_tag="VEJ00_01530"
     CDS             complement(7075..>7491)
                     /locus_tag="VEJ00_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99867.1"
                     /translation="LAGSVFLVDSIGELAALYALADVAFVGGSLVPRGGHNIVEPAQH
                     GVAIVVGNHTENFRDIVGLFQSRDALRIAGFAELPLTLMHVLANEGERKALGRRALET
                     MRSQAGATSRTLAMLQTLVAEDRDAQAVGAQAAHTD"
BASE COUNT         1454 a         2506 c         2168 g         1363 t
ORIGIN      
        1 tccatgtgct ggattgggcg aggaggtcct tgttgtacgt ctgcctgatc tccccgggag
       61 catcctcggc tgggtacggg tgatcttcaa accacttgat gaacgaaggc ggagcccaag
      121 aactggggtc acgactcgcc cgttcctctt ttgcccaagt ggcgttcttc tcatcattgg
      181 taagcttgta accctcttct aggacgtcgt accactgccg ctttgactgc atgttcagcg
      241 cccggattcg ctggatgtcg cgcgccgttt gtgcgcgctg ccccgcgtat tccgaagagg
      301 gagtcgcttt gaactccagc gtccagaggg ttcggtaggc ggcgatcgca tcgatgtctt
      361 cgcgtttctc gatcagagca cgcaactggg cggctctttt gcccactgct gcctgatcct
      421 catcggatcg cgccagctgt tcgtacccct cgaaagacgc cgggcattga gcaagaaacg
      481 ctctcacgtg ctcctccgcc ttgcttctgt cgcgaaatac gggagccgaa taaatccaga
      541 acagcggaat gtgtggccag gggaactggg gatctttttc cagcgcgcta tccagcagtt
      601 tgatcgattg cggagaatca cggccgaaga gcacgactcc gtagaggtaa gcggccagtg
      661 gactgccggg actggcgatg tacctggctt tatattcttc aattactcct gggcgatcga
      721 ctctgtcgtg catggacttc atatagcgcc gctcaataaa aacgttgtcc ggatgctggc
      781 ggcgaagcgc ctgaacagcc tcaagttctt tccggtggaa ctcccactcg gttagctcgt
      841 gggacggctc cgggagcgcg tcaagccccg ccttcacttc cgatgaagga ctgcagtcgt
      901 cccactgttg tgcgcgcgct agggaaaaga taccgctcgc aaacaacatc agagcgaggc
      961 agccatggac aaaactgatt cgcagctggc ccaggatctt tgcagccgcg acggcgttga
     1021 atgttcttag cgggggcccg ttggagacga gaatcggact tttcgccacg gtggctcctg
     1081 ggagactgac tggcccagac ctgccggtcc agcgttcagc cttgtgaaac agatgctaac
     1141 atgcaaccag ggaatgtctt gcggtcgaac ctccaactgt tcttagccat gtttgacccg
     1201 tccgccgcca cgttactaga atcgcttcac agaccactgt ggggtccgta tgaaaaaagc
     1261 cgcctgcctg ctgttgctct ccgtcttctt cttcacgctc gctgccttcg ccgatgacga
     1321 tcacgatatg catcaccacg aagccctgac cgaaactcag ctgggcacgg tccatttccc
     1381 cgtctcctgc gcggagtcgg tgcaaaagtc cttcgctcgc ggcgtcgcgc tgctgcactc
     1441 attctggtac gaggaagccg agaaggaatt cctcgacatc tccaaggacg atcctcactg
     1501 cgccatggct cactggggag tcgctatgag cctgtggcac cagctctgga acgagcctga
     1561 cgccaaggtc atcgcccgcg gtctcagtga agccaaggcc ggcgagaagc tggcaagaaa
     1621 agccacccca cgcgagcagg cctatctcgc cgccatcgcc gctttctaca gccactccga
     1681 caagctcgac cacgctgccc gtgccaaggc ctactccgag gcgatgagga aggtgtacga
     1741 atcctatccc gacgatcacg aagcggcgac cttctatgcg ctctcgttgt tggcgtcgga
     1801 gccgcacaac gatgagacgt tcgccaatcg gaagcaagcg gctgcgattc tggaaaagct
     1861 gttcgccatc gaacccgacc accccggagt ggcgcactac ctgatccaca gctacgacaa
     1921 gccgcagttg gctcagcttg gcatccccgc cgcccgccgc tacgcgcaga ttgccccagc
     1981 cgcgccccac gcgctgcaca tgccttcgca catcttcgct cgcgtcggcc tctggcagga
     2041 cgacatcaac tccaacctgg cctccgtcgc cgccacgcgc aagaccgcgg ccatgggcat
     2101 gggcggcgaa ggccaccagt tccacgccat ggacttcctc gtctatgcct acctgcagag
     2161 cgggcgggaa gccgaagcgg ccaagatcat cgacgaagtc aagaccatgc ccccgatgca
     2221 cgacatgtac ggtatgggct tcgacccgcg cacctacgcg ctctccgagt tcccggcgat
     2281 ttacgacctc gagctccgtc attggagtga tgccgccgcg ctcgtccccg tcgaaggagc
     2341 ccatggcagc agaagcgtca ccttctgggc gcgtgcgatt ggagccgcgc gcagcggcaa
     2401 tgtcgtgcag gccaagcaag acctggaaca gatcgaagcc gtccgcaagc agatgctggc
     2461 cgacaagaat acagacttcg ccgaagccgc ggagcaggac tatcaggaag cctcggcatg
     2521 gatcgcccat gccgaaggca aagacgatga agccatcgcc acccttcgca cggtggctga
     2581 caagaacgac aagttgggcg acgagccgcg aaccatccct gcacgcgaaa tgctggccga
     2641 cttgttgctc gaaaccaagc gcccgcagca ggcgctgacc gagtatcaga ctgacttgaa
     2701 gatcaatccc aaccgcttca acggcctcta cggcgccgcc cgcgccgctg aagaagccgg
     2761 aaagcagagt gacgcaaacg agtactacgc gcttctgctg aagacctgcg aaggcggaag
     2821 ctctacgcgg cccgaactga gccgggcaaa ggagttggtg gcgcagaaat aacctcaaga
     2881 aagccaagtc atcttggcga tgagcgcacc cgtcagcagg gcactcccca gcagcgcgtt
     2941 ccactcccgg tgcttcaggt acagcgccat cgaaaatccg ccgccctcgc caccaattcg
     3001 tgctggcacg atccgcggca gcatcctcgg cacctgccgc gcatatacct caaactcagg
     3061 aaacttttct cgcaggaacg cttcttcgtc gcgaatgacg ggaaggtaaa tcacaacaag
     3121 catcacgacc agggcaccgc cgatccacca gctgcgcgcc gctacggcga atcccatgcc
     3181 gatcagaagc gagccaaggt aaagcggatt gcgcgtgtaa gcgtacggtc ccgatgtcgc
     3241 cagcgcctcg ttcttccgca catgccccga agccagcgcg cgaatcagaa gccccggcac
     3301 aatcagcact gcaccgaacg caaggaaccg ccacgtcggc cgcgcaagcc agaagtagaa
     3361 cacggcaaac gcgaacccca gcggcacgcg aatgcggcga gcgatctttg cccatttagc
     3421 gcgcgaccac tcagccatgc gcaccgtctt tcagtgtcgc gtgagtactc tcgccttcgt
     3481 ggcccgaact cgatagcaac ttgcgcgccg cttccacaac cgcgctgacg ctgatctcta
     3541 gcaaaccctc atcaggctgg gcacgccgcg cgtgcgtggt cggactcgcg ggattgcgca
     3601 acacaatgct tctcgtggcg taaggcccgt tgcgcgtggg atccgtcggc ccgaagatcg
     3661 ccactaccgg aacctgcagc gccgccgcaa ggtgcatcgg cccagtgtct cccccgacga
     3721 acaacctggc ccggcgtgta agggcgatca attcgctgac ggagcactcg agggccctgg
     3781 cagcacctcc gctggcagct tcggcatcgc gagccaatgc ttcctcgccc ggaccatagt
     3841 tgagaaacga ctggattccg tctgcggcca aacctcttgc cacttgaccg tatctctcca
     3901 ccggccagcg cttcgccccc cagcccgccc cggggctcag gatgatgaag tcgcgaactc
     3961 ccagttccgc caattgctga tcaatccgac gctccgcctc ggcatcatgc ggcaactcca
     4021 cgcgctgcac cgtcaatttc ttcttcgcaa ccgcctccac aacagccaga ttttgctcga
     4081 ttacgtgtgc gccgccgcct cctgcccggc ttgtgtacca gaggctggcc ggagactccc
     4141 gcgcttcagc ggcaccatac accgcgcgcg cccccgacca gcgcgccagc accgccgatc
     4201 gtatggcgcc ctgcaggtca acggcaacgt catagcgggc gctgcgaacg tcgttccagg
     4261 ttttcgcaat ctcctgcagg gtcggaagcc cggccaggga actccgccat ttgcgcagat
     4321 tgacggtgtg cacccagtcc accaggggcc gccgcgccga acgcggcccg cggcgcgggg
     4381 tccccggtgc gcacagcaat tcagcccagc gctcttcaat cagccagccg atcatcgccc
     4441 gcgggaaggc tgagcgcagc gcttgcgccg ccggcagagt atgaatcaca tctcccatgg
     4501 cgctcaggcg tacgatcagc aggcgctcga ccacaccacc atcatcgctg tttgcgctca
     4561 agatttcctc ggcgaccgct gggcgcgaat gatttcgctg gtggagtgat tcttcggatc
     4621 gccgacgatc gctacacggc ctccgtactc ggcgaccgca tcgcgttccg gcacagattc
     4681 ttccgtatag tcggtgccct tggcgtgtat gtcagggcgg atctcgcgga tgagcgggcg
     4741 cacatcgagc tcggagaaga tcacaacggc atcgacgtcg gccagcgcgg ccacgatttc
     4801 ggctcgttcc tcggccggca tgatcgggcg cccctcaccc ttgagcgcgc gcaccgattc
     4861 gtcggaattg acggcaacca ccaatttccc tcccagcgcc ttcgctcccc gcaagtagcg
     4921 cacatgaccg acgtgcagca agtcgaagcc gccgttggcc agcgtcacgc gctcgcccgc
     4981 gccgcgccac tcttccacgc gtcggcggag ttcttcgcgt gtcaggattt tgtcgtggga
     5041 ttgccctggg gtacgcatca atgaggccgt gtcgctggcg gcgcctgctc cagcgcgtgc
     5101 agcagttcgt ccatcgaaac cgtagccgtg ccgcgcttca tcaccacgat cccgcccgca
     5161 tagttggcca gttgagccgc ctcttccgtc gtcgctccgg cggcgagcgc ggccgtgaag
     5221 gccgcgatca cggtgtcgcc cgctccggtc acgtcggcaa cctggtcaga accgtagatc
     5281 ggaatatcca ccggcttgtg cttgtgatcg aatgccgcca tgccatcgcg gccccgcgtg
     5341 atcaccagcg actgcagctt catgcgcgcc atcaactgct cgcccgcggc cagaagcttg
     5401 gcccagtctt ggccgatgcg gaggccgagc gcttcctcca cttccggttc gttcggcgtt
     5461 gccgccgtga ttccagaata ttcgagcagg cgatagcgcg agtcgagaac cagcggcagc
     5521 gctcccagtt ttcctttctg ctgcagagca tcgacgagag ccggggtcgc ggcgccgtag
     5581 ccatagtcgg aaacgagcaa cgcatccgaa gcatgagcat agttgcgcgc ggcaagatac
     5641 aattcgcgcg tcaggtgggc atttggcgct tcgtgcggct cgcgatcgac acgcaccacc
     5701 tgttggcggg cagtatgcgt catccccgcg agaatgcgcg tcttggtgat cgtggagtag
     5761 ctcttgtcct tgaggacgcc gctgacggcg attcgcttgt ggcgaaaata tttcagcagc
     5821 agccgacctg gctcgtcgtc gccgacaatt cccacgggaa gaacgttgac tcccaggtcg
     5881 gccagattgt tcgcggcatt ggctcctccc cccggcacta ccgtgcgttc gcggtgcttg
     5941 aggatcagca cgggagcttc acgcgacaca cgcgaaatct cgccaaaaat aaattcgtcg
     6001 gccaccagat cgcccagcac tgtcaccgtg acgttgggaa acgcctcgac gatcttcctg
     6061 agccgctcgc cctctttggg atgcttggtc atgttcagcg gtgtagccgc gccgaagcta
     6121 cgcgcagatt ttctcatgcc cggcgcttcc gttcctcaat cacgcgcagc atggtatcga
     6181 gagcgttagt ggcgtcggca agttccatgc ggaccgggat gaccgagagt ggctccagcg
     6241 ccgagagata gggcccgagg ttaattgcat ccttctcggt cgtcacgaac ccgccggcct
     6301 cgcttttctg tttgagcgcc agaagctccc gcacgtcttt ctcgctatag gcgtgatgat
     6361 cgcggtaaaa cgcttcggcc gcgggctcga tctgcgccgc tcgcagttgc aacacgaaag
     6421 tctgcggccg cgcgatgccg caaaagacca caggcctcgg cggagcgttc tgggtcgcaa
     6481 ttccccggcg cactcgccat accagctttc ccacgagagg gaacgattcg gcagttgcgc
     6541 cgctggcaag aacgatcgag tcagcccggc tcagggaagc aagcggctca cgcagccggc
     6601 cggcaggaag cagctgatcg cttgcatcct gcggcgtaac cagcacaatg tcgaaatcac
     6661 gcgccagcat gcgatgctga aagccgtcat ccagcagatg catctgcggg ccgaacttcg
     6721 actcggccag gcggccggcc tcgtagcgct cctctccgac aatgaccgaa acccgaagct
     6781 tgcgcgcgat cagcagcggc tcgtcgccga aatgctgcgg caggccgccg gggtcgacca
     6841 gcagcgcgcc gcgggacttg cgcccgtagc cgcgggagag tacgtcgaat cgcacaccac
     6901 gagctttcag cagttccccc agcagcatca cgaagggagt cttccctgac cctccggccg
     6961 agagattccc gacgctgact acgggaccct gcaggcggtg cgcgtgggag aagccccggt
     7021 catagagggc gtttcgagcc ccgaccatgc cgccgtagat ggttgagagc ggattcaatc
     7081 agtatgtgcg gcttgtgccc caactgcctg ggcatcgcgg tcctccgcga cgagcgtttg
     7141 cagcattgcc agagtcctgg acgtcgcgcc ggcctgagat cgcatggtct ccagcgctct
     7201 tctgccaagt gccttccgct cgccttcgtt cgccagcacg tgcatgagag taagaggcaa
     7261 ctcggcaaag cccgcgatgc gcagcgcgtc ccggctctga aacagtccca caatgtcgcg
     7321 gaaattctcc gtatggttgc cgaccacgat ggcaacgccg tgctgcgccg gttcgacaat
     7381 gttgtgcccg ccacgcggca ccaggcttcc accgacgaac gcaacatcgg ccaaagcata
     7441 gagtgctgcc agttccccaa tggaatcgac cagaaacacg ctccctgcca g
//
LOCUS       DASPUL010000065         7121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_832147, whole genome shotgun sequence.
ACCESSION   DASPUL010000065 DASPUL010000000
VERSION     DASPUL010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832147"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..651
                     /locus_tag="VEJ00_01535"
     CDS             223..651
                     /locus_tag="VEJ00_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99868.1"
                     /translation="MTSTGKTRSMVGLVALAGALVAFLPAARALSRIIELTADSDSRY
                     KMAGMRVPEITVKAGEPVVLRVMARKGKSWNRDGSIHGFTLLRAKERTKVEGWDLLFK
                     PGLQEFHLTAPLEPGEYEVVCTVICSEDHEGMHMKFTVLP"
     gene            673..1044
                     /locus_tag="VEJ00_01540"
     CDS             673..1044
                     /locus_tag="VEJ00_01540"
                     /inference="COORDINATES: protein motif:HMM:NF039244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HXW99869.1"
                     /translation="MNRAIIWYVLGVLVLAGTGLGQSPRVFHGEISDSQCALNVHSLT
                     RSHQEMLKSKSMGGTANTCTLYCIEHMGGYLVLAAGKDVYRLDRADLVHGFEGQRVKV
                     TGTLDTKLNLIHVLKIDLDEQ"
     gene            complement(1070..2371)
                     /locus_tag="VEJ00_01545"
     CDS             complement(1070..2371)
                     /locus_tag="VEJ00_01545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXW99870.1"
                     /translation="MESTSPAGAGVRDHGSPAGLWARTRSSPFSIFAIAFVLRVSWIL
                     IGHTYKFKSTDNSFGFGWEMGRIAAALASGQGFSNPFGPPTGPTAWEPPFYPYLTAGV
                     FHIFGTYSRASAFAVLALNSLFSALTCIPIYRIARRMFSEPVAIGSAWAWALLPNVIF
                     WCTRAVWETSLSALLFATLIWLALILEERDGLQPWLEFGLLWGIAALNSPSLLSFLPA
                     SGLWVWYRRVRSGKRSLAGIALASIVFLACITPWLVRNYHTFGKFIFIRDNFGAELRL
                     GNGPGADGTWMEYLHPTQDAYAMRQYQTMGELAYIVMRKQQAVDYIKADYARFAGLCV
                     KRFIYYWAGPAKNLQSAGLGMVKNSLFLAWSVLTFWGLARALRQRKPGAWLLFWLILL
                     YPAVYYVVFPAPRYRHPIEPVMAILGVFLLTEAGKPRRAGL"
     gene            2374..3000
                     /locus_tag="VEJ00_01550"
     CDS             2374..3000
                     /locus_tag="VEJ00_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99871.1"
                     /translation="MALFVNDGISVSKLARTGVQCPATARRCVMILGMSTSTYTMLHV
                     LISFVGIGSGLAVMYGFLRAKRLDAITAIFLVTTALTSVTGFGFKIEHLTPGLVVGAL
                     SLVLLAIAIAARYRLHLRGAWRPIYVVTAAIALYLNVFVLVVQSFEKVPALNALAPTQ
                     KEPPFAVAQLTVLAVFIAATIVAVRKFHPDIAVNPASLSDGRREKGAA"
     gene            3091..3456
                     /locus_tag="VEJ00_01555"
     CDS             3091..3456
                     /locus_tag="VEJ00_01555"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXW99872.1"
                     /translation="MVNRRADVSSVFAALADPTRRRILERLAGSGECRVTALAKPFRM
                     SLPAVSRHLRVLEQARLIRRQRHGREHLIRANVPGLEGAHKWIMQCALHWDSRFDKLD
                     ELLRNEPISAAQRKENHEI"
     gene            3446..3892
                     /locus_tag="VEJ00_01560"
     CDS             3446..3892
                     /locus_tag="VEJ00_01560"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXW99873.1"
                     /translation="MKSDVRITGNRLQIARVFDAPRERVFAAWTRPELLQQWSGCSDT
                     TKVEFQIDFRVGGSFVQKMHISGVGEYTITGAYDEIIEPEKISYHVDLGPATTRVTVE
                     FIAQGQQTKVVLTQEGFPDPNLCKTVSQGTVEALDKLDQLLMAQAA"
     gene            3950..4366
                     /locus_tag="VEJ00_01565"
     CDS             3950..4366
                     /locus_tag="VEJ00_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99874.1"
                     /translation="MNYGRILLAAVGGMVVFFILGALIQATPLAKMYGAYPGVYREKE
                     AIMSRMPIGMSGMFVAILAVTLIYAMSYSGGSGIAAGARLGLLVGIFAACAFEAHSFT
                     ILNIGGKLALGLAAAAIVQWTVIIIAIGLIYKPFPN"
     gene            4559..5296
                     /locus_tag="VEJ00_01570"
     CDS             4559..5296
                     /locus_tag="VEJ00_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXW99875.1"
                     /translation="MGVKERREREKSETRDKILDAARELFFTEGYEGVSMRKVAEKIE
                     YSPTAIYVHFADKEELFRELCHQDYAALAQVFQSSVISTDPVERLKQIGAIYIDFGTR
                     YPNHYKFMFMTPHPAQELDDADCDVHGNPEKDAYAFLRWAVQQAIDTGSFREGLRDAE
                     LISQTLWASVHGVISLEIAKGRDAWVNWRPVGQRAELMLDVTLRGLVREQAAGRSPLP
                     TQNAGNGAPGQSAAESPIVPDIPTEEK"
     gene            5299..6423
                     /locus_tag="VEJ00_01575"
     CDS             5299..6423
                     /locus_tag="VEJ00_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW99876.1"
                     /translation="MPPLAQRNLFHDKVRLTVTLTGIVFSVVLIVVQLGLFIGFTTAT
                     SGLIDHSGADLWITSKNVPYVEQGVAFSERKLNQVRAVPGVADAQKIIAHWTQWKRHD
                     GGEESVQIVGINVDDPLERPWNLVEGRVEDLKSPDAVILDELYKQKLGVSHVGEVFEI
                     GGYRARVVGFTRGIRSFTTSPYVFTSFKNAQDFTKLREDQTLFILVRVAPGADVQQVR
                     RDLLAHVKDVDVLTSAEFSHMTTFYWMFTTGAGVAVLIAAALGLVVGFVVVAQTIYAT
                     TMDHIREFGTLKAMGAPNSYVYKVIMKQAAISAVIGYVLGMIVSVFVVQASQKGGAAI
                     LMPAPMAVGMFFLTLAMCVGAALVSINKVTRIDPAMVFKG"
     gene            6500..>7121
                     /locus_tag="VEJ00_01580"
     CDS             6500..>7121
                     /locus_tag="VEJ00_01580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW99877.1"
                     /translation="MQPAIEVRQLTKKYEEGTSGTLALRGVDLDVQAGELLMLMGPSG
                     SGKTTLLSIMGCILTATSGSVRVAGREVVGLREKDLPALRLEHIGFVFQGFNLFPTLT
                     AGENVELMLDLKGISTAKAKKRAEELLEQVGLGEKYGSFPSDLSGGQKQRIAIARALA
                     GDPGIILADEPTAALDSLTGRSVMEMMSDLAHKRGRSVVVVTHDSRV"
BASE COUNT         1440 a         2020 c         2282 g         1379 t
ORIGIN      
        1 agacagcatg cgggtgcagg gtgagcagcg ttttcacgcc aactgcgggc gctgtcacgc
       61 ggcgccgcat aagtttccgc cgcgtgtgat ggcgactgtg atccgccaca tgcgcgtccg
      121 ggccacgatt actgacgaag atatgcgctt gatcctcttc tacatggcgc agtgaccgcc
      181 gtttgggtat gcagtaacca gggagataag caggaagaaa ctatgacaag cacgggcaag
      241 actcgttcaa tggttggttt ggtggcgttg gcgggcgcgc tggtcgcgtt cctgccggcg
      301 gctcgcgcgc tatcccgcat catcgaactc acggccgaca gcgacagccg ctacaagatg
      361 gccggaatgc gcgtccccga aattacggtc aaggccggcg aaccggtcgt tctacgggtc
      421 atggcgcgca agggcaagtc gtggaatcgc gacggctcta tccacggctt caccctgctg
      481 cgggccaagg agcgaaccaa ggtggagggc tgggacctgt tgttcaagcc cgggctgcag
      541 gagttccacc tcaccgctcc tctcgaaccg ggggaatacg aggtcgtttg cacggttata
      601 tgcagcgaag accatgaggg catgcatatg aagtttacgg tgctgcctta aggcttcaga
      661 atctgaggtt gtatgaatcg agcaatcatc tggtacgtgc ttggagttct ggtgctggca
      721 gggacaggcc tggggcagag cccgcgcgtt tttcacggag agatttccga cagccagtgc
      781 gcgttgaacg tccactcgct cacccgctcc caccaggaga tgctgaagtc gaaatccatg
      841 ggcggcaccg cgaacacctg cacgctgtac tgcattgaac atatgggcgg gtatctggtt
      901 ctggctgccg ggaaagacgt ctaccggctc gaccgcgccg acctggtgca cggtttcgaa
      961 ggacaacggg tcaaagtgac cggcactctc gacacgaagc tcaacctgat tcacgtgctg
     1021 aaaatcgatc tcgacgagca gtgacaggct gttggtgttc agtcctggct cacaggcctg
     1081 ctcgccgcgg tttgccggct tcggtcagca ggaagacgcc gaggatcgcc atcaccggtt
     1141 cgatcggatg acggtagcgc ggtgcaggga agacgacgta atacaccgct ggatataaca
     1201 gaatcagcca gaacagcagc caggcgcccg gtttgcgctg ccgcaacgct cgcgccagac
     1261 cccagaacgt cagaacggac caagccagaa acagcgagtt cttcaccata ccgagtcctg
     1321 ccgactggag atttttcgcc ggtcccgccc aatagtagat aaatctcttc acgcataggc
     1381 cggcgaaccg ggcatagtcc gccttaatgt agtccacggc ctgctgcttc cgcatcacaa
     1441 tgtaggccag ctctcccatg gtctggtatt gccgcatggc ataggcgtcc tgggtcgggt
     1501 gcagatactc catccaggtt ccgtcagcgc cggggccgtt ccccagccgg agttcggcgc
     1561 cgaaattgtc acgaatgaag atgaacttcc cgaaggtgtg gtaattgcgg accagccacg
     1621 gtgtgatgca ggccaggaaa acgatcgagg cgagagcaat cccggccagc gatcgcttgc
     1681 cgctccggac gcggcggtac caaacccaca atcccgaagc cggcagaaac gataagagcg
     1741 aggggctgtt cagggctgcg atgccccaga gtaatccgaa ctcgagccac ggctgcaggc
     1801 cgtcgcgttc ttcgaggata agagccagcc agatcagcgt ggcgaacaac agcgccgaca
     1861 ggctcgtctc ccacaccgcc cgcgtgcacc agaaaatgac gttgggcagg agcgcccagg
     1921 cccacgcgga gccgatcgcg accggctcgg agaacatgcg gcgggcgatt cgatagatcg
     1981 ggatgcaggt cagcgccgag aagagactgt tcagcgcgag cacggcgaac gccgaggccc
     2041 tggaataagt cccgaaaatg tgaaacacgc cggctgtcaa gtagggataa aacggtggtt
     2101 cccatgctgt cggtcccgtc ggagggccaa agggattgct gaagccctga ccggaggcaa
     2161 gcgcggccgc gatgcggccc atttcccagc caaacccaaa actattatcg gtgctcttga
     2221 acttgtaagt gtggccgatg aggatccagc tcacgcgcag gacgaaggcg atcgcgaaga
     2281 tagagaatgg ggagctgcga gtgcgcgccc acaggcccgc tggcgagccg tgatcgcgta
     2341 cgccggctcc ggccggggag gttgattcca tgagtggctc tatttgtaaa tgacggcatc
     2401 agcgtgagca agctggcgcg aaccggcgta caatgcccgg caactgcacg gaggtgcgtc
     2461 atgattctgg ggatgtccac ttcgacttac acgatgctgc acgttttgat cagctttgtg
     2521 gggatcggtt ccggccttgc cgtgatgtac gggttcctga gggccaagcg gctcgacgcc
     2581 attacggcga ttttcctggt gactacggcg ctcaccagcg tcacgggctt tggcttcaag
     2641 atcgagcacc tgacgcccgg tctggtcgtc ggcgccctgt cgctggtgtt gctggccatc
     2701 gcgattgcgg cacgctaccg gctgcatcta cgcggtgcct ggcggccgat ctatgtcgtg
     2761 accgctgcga tcgcgctgta cttaaacgtc tttgtgctgg tggtgcagtc gtttgagaaa
     2821 gtgccggcgc tgaacgcgct ggctccgacg cagaaggaac ctccctttgc cgtggcgcag
     2881 ctgacggtgc tggcggtttt catcgcggcc accatcgtag ccgtgcggaa gttccatccc
     2941 gacatcgcag tgaacccggc gtctctgtcg gatggccggc gggagaaggg cgcggcctga
     3001 atccctctgc gcctgggcga ttgtaaaatt taacgcaatc gttaactatt tccttgacac
     3061 gcgtcaccgt gcggcccaat atttaacggc ttggttaacc gtcgagctga tgtctcttcc
     3121 gtatttgcgg ctttggccga tcccacccgg cgccggatcc tggagcgcct ggcgggcagc
     3181 ggcgaatgcc gggttacggc cctggccaag cctttccgca tgtcgctgcc ggccgtttcc
     3241 cggcacctgc gggtgcttga acaggcccgg ctgatccggc gccagcgcca cgggcgcgaa
     3301 cacctcatcc gcgccaacgt gcccggtctg gaaggcgccc acaagtggat catgcagtgc
     3361 gccctgcact gggactcgcg cttcgacaag ctggacgagt tgctgaggaa cgaaccgata
     3421 tccgccgccc aacgaaagga aaaccatgaa atctgacgtc aggatcaccg gcaaccggct
     3481 gcagatcgcc cgcgtgttcg atgctccccg cgaacgtgtt ttcgctgcct ggacgcggcc
     3541 cgagctgttg cagcagtggt ccggttgcag tgacacgacc aaagtcgagt tccagatcga
     3601 ttttcgtgta ggcggctcct tcgttcagaa gatgcacatc agcggagtcg gcgagtacac
     3661 gataaccggc gcgtacgacg agattatcga gccggagaag atctcctatc acgtcgatct
     3721 tggccccgcc acgacgcgcg tgacggtgga gttcatcgcg cagggccaac agaccaaggt
     3781 tgttctgacg caggaaggct tccccgatcc gaacctgtgc aagacggttt cgcagggcac
     3841 agtggaagcg ctcgacaaac tggatcagtt gcttatggcg caagcggctt aggcgtgtcg
     3901 tggcggcagg ggggcacacg gtaatccaat ctgctttcag ggagcgcaca tgaactacgg
     3961 acgaatactt ctggctgccg ttggcggtat ggtcgtgttc ttcattttgg gagccctgat
     4021 tcaagccacg ccgctggcga agatgtatgg cgcctatccc ggcgtatatc gggagaaaga
     4081 ggcgatcatg agccgcatgc cgatcggcat gagcggtatg ttcgtcgcga ttctcgcggt
     4141 gactctgatt tatgcgatga gttattcggg cggaagcggc attgcagccg gcgcacgcct
     4201 gggcttgctc gtcggaatct ttgccgcctg cgcatttgag gcgcacagtt tcacgatcct
     4261 caacattggc ggaaagctgg cgctggggct ggcagcagcc gccatcgtgc aatggaccgt
     4321 gatcatcatc gccatcggac tgatctacaa accctttcca aactaatctg cctgtagaac
     4381 caccgctgcg cctccccgct tccatatagg atattatcaa ctttatttac aatcggttac
     4441 aaggatttcc acaaatcacg cgaaattttc gcttgacata gatgaacgat gcgcagtaac
     4501 ttaacgttgt taactaaaca ccgttaacca aaggatctgc gttacgaggt acccggaaat
     4561 gggagtcaaa gagagacgcg agcgcgagaa gtcggagacg cgagacaaga ttctcgacgc
     4621 ggcgcgcgag ctgtttttca ccgagggcta tgagggcgtc tccatgcgca aggtggcgga
     4681 gaagatcgag tactctccga ccgccattta cgtgcacttc gccgacaagg aagaactgtt
     4741 ccgcgagctt tgccaccagg attatgcggc tctggcccag gtgttccaga gttcggtgat
     4801 ttcgaccgat ccggttgagc ggctcaagca gatcggcgcg atctacatcg atttcggcac
     4861 gcgctatccc aatcactaca agttcatgtt catgacgccg catccggctc aggagctgga
     4921 tgacgctgac tgtgacgtac acggcaatcc ggagaaggat gcgtacgcct ttttgaggtg
     4981 ggcggtacag caggcgattg acacggggtc gtttcgcgaa gggctgcgcg atgcggagtt
     5041 gatctcgcaa acgttgtggg cctcggtgca cggcgtgatc tcgctcgaaa tcgccaaagg
     5101 gcgcgacgcg tgggtgaatt ggcgtccggt aggtcagcgg gctgagctga tgctggatgt
     5161 gaccttgcga gggctggttc gggagcaagc cgctggccga tccccacttc ccacgcagaa
     5221 cgcgggaaat ggggcacccg ggcagtccgc tgccgagtcg cccatcgtgc ccgacattcc
     5281 cacggaggag aaataatcat gcctcccctg gcacaacgga atctgtttca cgacaaggtg
     5341 cggttgacgg tcacgctgac gggaatcgtc ttctcggtcg tgctcatcgt cgtgcagttg
     5401 ggcctgttca tcggcttcac gacagctacg tccggattga tcgatcactc gggcgcggac
     5461 ttgtggatca cctcgaagaa tgtgccctac gtcgagcagg gcgttgcctt cagtgagcgc
     5521 aagctgaacc aggtgcgggc ggtgccgggc gtggccgacg cacagaagat catcgcgcac
     5581 tggacgcagt ggaagcgcca cgacgggggc gaggaatcgg tgcagattgt cggcatcaat
     5641 gtcgacgatc cgctggagcg gccgtggaat ctggtcgaag gacgcgtcga ggatttgaaa
     5701 tccccggacg cggtcatcct cgacgaactc tacaagcaga agttgggcgt aagccacgtg
     5761 ggcgaggtgt tcgagatcgg cgggtaccgt gcccgggtag tgggcttcac gcgcggcatt
     5821 cgctcgttca caacctcgcc ctacgtattc acctcgttca agaacgcaca ggacttcacc
     5881 aagctgcgcg aggaccagac gctgttcatc ctggtgagag tggcgccggg cgcggacgtg
     5941 cagcaggtgc ggcgcgatct gctggcccat gtgaaggatg tcgatgtgct cacctccgca
     6001 gagttcagcc acatgaccac tttttactgg atgttcacca ccggcgcggg cgtggccgtg
     6061 ctgatcgcgg cggcgctggg gttggttgtg ggattcgtgg tcgtagcgca gacgatttac
     6121 gccaccacca tggatcacat ccgcgagttc ggcacgctga aggcgatggg cgcgcccaat
     6181 agctacgtct acaaagtcat catgaagcaa gcggcgatca gcgcggtgat cggctatgtg
     6241 ctagggatga tcgtcagcgt gttcgtggtg caggccagcc agaaaggcgg cgcggcgatc
     6301 ctgatgccgg cgccgatggc cgtcgggatg ttctttctga cgctggccat gtgcgtgggc
     6361 gcagcgctgg tttcgatcaa caaagtgacg cggatcgatc cggcgatggt gttcaaaggg
     6421 tgagattctc gctgcgggat cgagggccgg attcctcgct tcgctcggaa tgacaacgat
     6481 ctgaaggaga gagtaaagca tgcaacccgc aattgaagtc cgacagctca ccaagaagta
     6541 tgaagaaggc acgagcggca ctttggcact gcgcggcgtg gatctcgatg tgcaggctgg
     6601 cgagttgctc atgctgatgg ggccgtcggg cagcggcaag actacgctgc tgtcgatcat
     6661 gggctgcatc ctgacagcaa cgtcgggcag cgtgcgcgtg gcgggcaggg aagtggtcgg
     6721 actgcgcgag aaagatcttc cggcgctccg cctggagcac atcggcttcg tgtttcaggg
     6781 attcaatctc ttccccacgc tcactgcggg cgagaacgtg gaactgatgc tcgacctgaa
     6841 gggcatttcg accgcgaagg cgaagaagcg ggccgaggaa ctgctcgagc aggttgggct
     6901 gggagagaag tacggcagct ttccatctga cctgagcggc gggcagaagc agcgcatagc
     6961 gattgcgcgg gcgctggcgg gagatccggg aatcattctt gccgacgaac cgacagcggc
     7021 gctcgattcg ctcaccggtc gcagcgtcat ggagatgatg agcgacctgg cgcacaagcg
     7081 tggacggtcg gtggtggtcg tgacccatga ttcgcgagtg c
//
LOCUS       DASPUL010000066        16523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_420674, whole genome shotgun sequence.
ACCESSION   DASPUL010000066 DASPUL010000000
VERSION     DASPUL010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 16523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16523)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16523
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420674"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VEJ00_01585"
     CDS             <1..667
                     /locus_tag="VEJ00_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXW99878.1"
                     /translation="DHTLGLLLLRELQPLRVYATASVRRILTEDNSMFAMLQRVRGQV
                     TWTDYDPGSTVALLNAAGEYSGLRFTSFSLGTRFPAYVSQERRWELRSEEASLGLTVR
                     SPSGKQLAYLPAVPEFASGAGSALDDADVSLFDGTFWSDDELIRTQGSGQTATQMGHV
                     PVFSDDYGDDGLSGFSAFGRSRKIYVHINNTNPMLNEAGPEYRQVREAGWEIAEDGWQ
                     FDL"
     gene            649..1404
                     /gene="pqqC"
                     /locus_tag="VEJ00_01590"
     CDS             649..1404
                     /gene="pqqC"
                     /locus_tag="VEJ00_01590"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HXW99879.1"
                     /translation="MAVRSVANTTDLLSFDELRAQLRAVGEERYHHRHPFHLMMHEGQ
                     LSRGQLQAWALNRYYYQSTIPIKDSIILSRGPDPAFRRAWRKRVVDHDGDATSDGGIK
                     RWLKLAEATGLDPEQVRTGTGILPATRFAVNEYLNLVRSRGLLEAVASSLTELFSRDL
                     ISLRMEKLRKHYPWLSGGLDYFEARLTQAPEDAQFAINYVYENAKTRPEQELAIQALR
                     DKCDILWAQLDALYFAYVQPGWPPPGAFQIGEE"
     gene            1407..1682
                     /gene="pqqD"
                     /locus_tag="VEJ00_01595"
     CDS             1407..1682
                     /gene="pqqD"
                     /locus_tag="VEJ00_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HXW99880.1"
                     /translation="MAPPSESSQPKLATGCRWGGSEENRVILYPEGAIKLQGTGRQVL
                     ERCDGTRTFSEIIAELQNEFGKTDPQKIRSDISGFLEQLQKKRIVDY"
     gene            1689..2822
                     /gene="pqqE"
                     /locus_tag="VEJ00_01600"
     CDS             1689..2822
                     /gene="pqqE"
                     /locus_tag="VEJ00_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HXW99881.1"
                     /translation="MLSNPLALIAEITHRCPLHCVYCSNPLELAGSRSELSTPEWINI
                     FKQSGQLGMLHVHFTGGEPLARADLTELIAAARAARLYTNLITSGIGLNEQRLQEIVG
                     AGLDHIQISFQDSREEAANWIAGAKSHAHKIELAQVIRRHKIAFTVNLVVHRQNLDHL
                     EEMIAFIERLNPERVEIAHAQYYGWALANRAALMPTRAQVETAQAVVSEAEKRLAGKI
                     RIDSVVPDYYAKYPKACMGGWGRKLMLVNPAGKVLPCHAAEVLPGMSFESVRERTLEW
                     IWNESSSFRRFRGEEWMPEPCHSCERRTEDFGGCRCQAFLLAGNASVTDPVCSLAPAH
                     RLVESALNEANLSAAVAQAVSASSFVQLQKQTTDWWSYRTNPE"
     gene            complement(2877..3671)
                     /locus_tag="VEJ00_01605"
     CDS             complement(2877..3671)
                     /locus_tag="VEJ00_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99882.1"
                     /translation="MIRKLATVPGLRVVLIPITLVTILAGTATVRAQQSAAPQTSAQP
                     AAQQQPENQQSSSQEASPEETTRRKKPQEYKNWNFNVGGGAATYSGTTRQFVRGGGAV
                     GAAGVARNLSKYFGLRADFQFDNLPLRASALEAAQAPGANSHVYTLMADPIINLPVTK
                     VWSGYIVFGASYYHRSGKLDSSTAIPGAACNGFFTWWGRCFANSLPLNGNFLTSVQNE
                     FGENFGGGVARKVNSRIEIYGEYRLLHGRNGGITTDLRPITIGVRW"
     gene            3925..5235
                     /locus_tag="VEJ00_01610"
     CDS             3925..5235
                     /locus_tag="VEJ00_01610"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HXW99883.1"
                     /translation="MQDRSSKPAVKTTPPGRAEPAVCRVGLIGFGTVGSAVARILCER
                     PNGFLRLTHICNRNVQRKKQPWVPKDVIWTDDVQSVLNSDIQIVVELIGGLSPAEQIV
                     RSALEAGKSVVTANKQLIARHGPDLLQLAASRGCRLEFGASVAGGVPVLPALRTGLSG
                     DRLHGIAGILNGTCNYILSRIESARIPFSEALEEAQARGYAEADASEDLDGGDARAKL
                     TILALAGLHSRVTPETVRARSIRPIDAVDFDYAAELDCTIRQISRADLKDDTLFAEVG
                     PCLVPATSPFGRVQKNLNLVLTSGQYGGDTAFLGAGAGGDPTAVAVVSDLMSVAESLS
                     AGARNAVTSVSAPNISSDFETPWYLRFFVRDRPGIVARLARILAEHHLNIDSVLQKPG
                     FDKAALPFVITLEPCRDSLLHPALEKMSELDFAIRPCLCLPILR"
     gene            complement(5312..5479)
                     /locus_tag="VEJ00_01615"
     CDS             complement(5312..5479)
                     /locus_tag="VEJ00_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99884.1"
                     /translation="MKSNNPFSPGKTEKGNEMSAPPSRFLDEKISDETDTEDKTDSAL
                     RPAGLKNPFWN"
     gene            5709..6680
                     /locus_tag="VEJ00_01620"
     CDS             5709..6680
                     /locus_tag="VEJ00_01620"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXW99885.1"
                     /translation="MKYRKFGGTGIEVSEMAQGLWGMGGWTGSSDQESIAAMQLAVDL
                     GCNFFDTAWAYGEGKSDSLLGEIMARNSGKRLYAASKIPPANDRWPALPEYKYQDVFS
                     AKHVFKYADLIRKKLGTDTIDVLQFHVWDDSWTDEPEFRSTVERLKRDKWIRWFGLSI
                     NRWEPENCIKALRTGLVDVVQVIYNLFDQSPEDELFPVCQELNIGVIARVPLDEGSLG
                     GKMTLETRFPKDDWRALYFGPENLAPTIERVEKLKKVVPTGMTLPEMAMRFVLSHPAV
                     TTTIPGMRKLQHVRQNIAASDMGPLDRSLLAELKKHRWDRRPAHWSD"
     gene            6812..9955
                     /locus_tag="VEJ00_01625"
     CDS             6812..9955
                     /locus_tag="VEJ00_01625"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXW99886.1"
                     /translation="MFKSTFLSFLTIIATLSPLYGQNPRGSLRGSVQDSTGARIPSAP
                     IVAQSVDSSLRREATSEDRGEFRIDDLPPGNYHITVNAAGFAQAEADVAIAVSFVREV
                     TVTLIPAIPAETVSVQAASSSITTEISDTSSAVRGGVVGSQDLRTLPLPARSFANIAY
                     LVPGTEPVEPSDPTKARITAVSTGGSSGLNNELSVDGADNSDDWIGGFLQNFSPDGVQ
                     EFAVRTSNEDADTGSTTAGSVVITTKRGTNVWHGSGAFYDRQAALNARFPIENPAPNP
                     KQPFSRQNYVGTVGGPIAGNKVWIFASFENVHENASIAYSPASTKQFDALRDLASEES
                     TTGIPSIAVPATVPIPFRDYISSLRFDWAQSEKSNWFLRTSQDSYLTHNNLVQQATLP
                     STGLTTHNNYWNVALGNTYAFNSNWLGNLVVDASLLHLTQTRNSNLGFAIQFPFSVTA
                     LTVSGFETFGDNQFATPITLFPDLRNQDKYQFRYDVTHATGDHSIKFGVDFIHEPVLG
                     GAFASTAEELIQYPSNPDCYISPTGADCGGITNPLPFYFTPPNPATACTGTIPIGVNC
                     TFTPVGNGSFSQNVQRLAFYAEDSWRVSHQLTVNYGLRYQTTWGLFIGSGRSQADNAA
                     FATLQALRIPVVPSVPQDYRKQIAPRLGVAYSPGSSGNTVIRAGFGLFYDDLAQNGWG
                     TAFQAVNAPPVPCVDPVQNPGGSENAGCIPGSALAGAGNLIGSSYKTPYAIHATGGVQ
                     HAFNERWLISADFVHEQGNHGYRAFPYAGGVNLLTPLFPASDTTDQQNVVPNVNVFES
                     DNRSSYNALMLHLQGNMHRFNLVANYTFSKAQTWGCLLGELFDYVDGVCPNEPNTPNA
                     GFLNAFGQGDYGPSGEDVRHRFVLAGTLHVPGGFELSTVTQVESARPITIVNAKNSDR
                     IYVNGAYTSLDEFRGTPYIQSDLRVTRPFKINERWRVDPFIEFFNLFNRNNPGANYAV
                     NVAQLPVPAAQMQPDPVTGITNVTSICTVADCSQTVPITSLKHLEIPEGALGDFFGPG
                     TTVGIPFAAQLGVRLTF"
     gene            complement(9984..10934)
                     /locus_tag="VEJ00_01630"
     CDS             complement(9984..10934)
                     /locus_tag="VEJ00_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99887.1"
                     /translation="MFALRGISVSFSVFVIVYGVLSLAVAVSWSKIRLRIGGFATRRL
                     ADSLFALRVFPLVGAATVTLALVVPSFLLLEPRAIDEPLGTIPLALGSCGVLLGILGA
                     VTAATALRRAAHGIAACTRGAQLVELPASVPVLRIPRPVPAMTVAGICRPKVLLSEAA
                     EFLLTAGELRIALNHEVAHVARQDNLKKLVLRLVPFPAMRGLEAAWLEAIEMAADDAA
                     VSDAGEALDLAAALIKLARLGPPETPSDLTAALVHNPVSAVNARVGRLIAWSTERREA
                     PCKRSPLYPLAAALVTLSVLAITYSQLLVQVHTATEWLVR"
     gene            complement(10934..11380)
                     /locus_tag="VEJ00_01635"
     CDS             complement(10934..11380)
                     /locus_tag="VEJ00_01635"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HXW99888.1"
                     /translation="MKGILGFRDSLPGLGPLEERLLEALWARGNATVRELLQNGYHDL
                     AYTTVMTTLDRLFKKNLLSRQTEGRAFRYSPRFSREELHREVALEAFRQLLGSSPGSL
                     PLSHLVEVLTERDARLLEELLQLVETKRRQLRRGDPRRAETRKEND"
     gene            complement(11553..11629)
                     /locus_tag="VEJ00_01640"
     tRNA            complement(11553..11629)
                     /locus_tag="VEJ00_01640"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11593..11595),aa:Arg,seq:tcg)
     gene            complement(11681..11756)
                     /locus_tag="VEJ00_01645"
     tRNA            complement(11681..11756)
                     /locus_tag="VEJ00_01645"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11721..11723),aa:His,seq:gtg)
     gene            complement(11874..13142)
                     /locus_tag="VEJ00_01650"
     CDS             complement(11874..13142)
                     /locus_tag="VEJ00_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HXW99889.1"
                     /translation="MQIGVYGSGYLGTVISACLADFGTPVSCCHPDRSRMVEMAQGNI
                     PFFEKNLKEIIRRNVRAGRLAYSTDIESFARKTQVIFLAEDKVEHLKDVAMRIARLAS
                     KPPILTIVTPAPVGTATALEKNIKDAGLQATIVTQPMFFTDGCAVEDFNWPDRLILGT
                     SSNEAVQVLKQIFHPLVMRGVPVIVTNASTAELVREAATAFVATKISFINEVAALCER
                     VNADAVNLALALGLDKKIAPRCLQPGAGMGGVFAESDMDSLVELAQSHGVSLKVLNAA
                     RDVNHLLAERLVEKITQALSSVENKEVGILGLAFKPNTNSVAGSSSLRLAETLVSKGA
                     HVRAYDPVAIPEAKSRLNGSVRYCDSAYAAAEGSDALVVGTGWPEFRALDFDRIKHLL
                     RNPVIVDTKNLLDSTRLRALGFEYVGVGRV"
     gene            complement(13323..13868)
                     /locus_tag="VEJ00_01655"
     CDS             complement(13323..13868)
                     /locus_tag="VEJ00_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HXW99890.1"
                     /translation="MQNSRYALVAYVRGPVGEFVESLRRELHPEQPHLAAHLTILPPR
                     SLQGSENSALQLLERICGQEEPFEVHLGGVETFIPATPTVYIRVDGAASRMCALHQRL
                     NAEALASQEEWPYIPHLTIVKMSGEQVAQNAFGIARERWLRYSGSRRILLEKLTFVRE
                     DTQNCWVDVAPVPLGRTLVSR"
     gene            14001..14077
                     /locus_tag="VEJ00_01660"
     tRNA            14001..14077
                     /locus_tag="VEJ00_01660"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14035..14037,aa:Pro,seq:tgg)
     gene            14307..16097
                     /locus_tag="VEJ00_01665"
     CDS             14307..16097
                     /locus_tag="VEJ00_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HXW99891.1"
                     /translation="MDSLAARKKAQLETVGQFKVFYQFHLTDQVKESGITFVNHVVDD
                     AGIDYKPVHYDHGNGIAVADVDGDGLYDIYFVNQVGGNELWKNLGGGKFKNITKEAGV
                     GLPGRISVSASFADIDNDGAQDLFVTTVLGGNVLFKNDGHGHFRDITKEAGLDLVSHS
                     SGAVFFDYNNDGLLDLLVCNVGKYTSDKKGAQGQYVGLPDAFGGHLHPQRFEYPVLYK
                     NLGHDRFKDETAAVGLRPTGWCGDATFTDPDGKGFPSLFILNMQGHDHYLENEGGKSF
                     VDKTEQYFPKTPWGAMGVKFFDFDNDGRMDLIVTDMHSDMSESVGVEREKLKSIMRWP
                     DSFTQAASHEDFIWGNAFYHRLENGQYEEISDRIGVENYWPWGVSVGDINADGWDDIF
                     IASGMSFPFRYGINSMLLNNRGERFLDSEFLLGIEPRKNIYVPWFDLDCSQKNLTDLN
                     QKVCAGRSGKIRVLSPRSSRSSVMFDLDNDGDLDIVTNEFNSAPQVLISDLAQQKAIH
                     WLKVVLVGTRSNRNGFGATVRLQAGGQTYTKYNDGKSGYLSQSVLPLYFGLGDAEKID
                     KVEVDWPSGQKQVVTTGLGENQVLRITESK"
BASE COUNT         3442 a         4967 c         4851 g         3263 t
ORIGIN      
        1 ggaccacaca ctcggcctgc tcttgctgcg cgaattgcag ccgctgcgtg tctatgctac
       61 ggcatcggtt cgccgcatcc ttacggaaga taattccatg ttcgcgatgc tgcagcgcgt
      121 tcgcgggcag gtgacatgga ccgactacga tcctgggagc accgtggctc tgctcaatgc
      181 cgctggcgag tattccggcc tccgcttcac ctcattctcg ctcggtactc gctttcccgc
      241 ctatgtctcc caagagcgcc gctgggagct cagatccgaa gaagcgtcgc tggggctaac
      301 tgtcagatct ccttcgggca agcaactcgc gtacctgccc gccgtcccgg aattcgcctc
      361 cggggcgggg agtgccctgg acgatgcaga cgtatccctt ttcgacggca cattctggag
      421 cgacgacgaa ttgattcgca ctcagggttc tggacaaaca gccacgcaga tgggacatgt
      481 tccggtcttt tccgatgact atggagacga tgggcttagc ggatttagcg cattcgggcg
      541 atcgcgtaaa atctacgtgc acatcaacaa cacgaatccc atgctgaatg aggccggccc
      601 cgagtatcgc caggtgcgcg aggcaggctg ggaaatcgcg gaggatggat ggcagttcga
      661 tctgtagcca atacgacgga cctgctgtcg ttcgacgagt tgcgcgctca actgcgcgcg
      721 gtgggtgagg agcgctacca ccaccggcat ccctttcacc tgatgatgca cgaaggacag
      781 ctaagccgcg ggcaacttca ggcctgggca ctgaatcgct actactacca gagcacgatc
      841 ccgatcaagg actcgatcat tctctcgcgc ggtcctgatc ccgcgtttcg tcgcgcctgg
      901 cgcaaacgag ttgtcgatca cgacggcgat gcgaccagcg acggcggaat caagcgctgg
      961 ctgaaacttg cggaagcgac cgggctcgat ccggaacagg ttcgcactgg aaccggaatc
     1021 cttcctgcta cacgtttcgc ggtaaacgag tatctcaatc tcgtgcgcag tcgcggcctg
     1081 ctggaggcag tagcgtcgtc gttgactgag ctattctcgc gagacctgat ttccctccgc
     1141 atggagaagc tgcgcaagca ttatccgtgg ctgtcgggcg gtctggatta cttcgaggca
     1201 cgcctgacgc aagcgccaga agacgcgcag ttcgccatca actacgtcta cgagaatgcc
     1261 aagacgcgtc ccgaacagga actcgccatc caggctttgc gcgataaatg cgacattttg
     1321 tgggcgcagc tcgacgcgct ctattttgcc tacgtacagc ccggttggcc tccgccgggc
     1381 gcttttcaga ttggagagga gtaaacatgg cgcctccttc tgagtcgagt cagccgaaac
     1441 tcgcgaccgg atgccgctgg ggcgggagcg aagagaaccg cgtgatcctc tacccggaag
     1501 gtgcgatcaa gctgcagggc acggggcggc aagtgctgga gcgctgcgac ggcacgcgca
     1561 ccttcagcga gatcatcgca gagttgcaga acgagttcgg caaaactgat cctcagaaga
     1621 ttcgcagcga tatcagcggc tttctggagc aactgcagaa gaagcgaatc gtggactact
     1681 agagcccgat gctttctaat cctctcgccc tgatcgccga gatcacgcat cgttgcccgc
     1741 tgcactgcgt ctactgctcg aatcctctgg aattggctgg ctcgagatcg gagctgtcga
     1801 cccccgagtg gatcaacatt ttcaagcagt ctggccagct cggaatgctg cacgtacact
     1861 tcacaggcgg cgaaccgctg gctcgagctg atctgactga actgatcgct gccgcccgag
     1921 cagcgcggct ctacacgaat ctcatcacct ccggcatcgg cctcaacgag cagcggctgc
     1981 aggaaatcgt tggcgccggg ctggatcaca ttcagatcag cttccaggat tcgcgcgaag
     2041 aggctgccaa ctggattgcc ggcgcaaagt cacatgcgca caaaatcgaa ctcgcccagg
     2101 tgattcgtcg gcacaagatc gcgttcaccg taaatctggt cgtgcaccgg cagaatctag
     2161 accatctcga ggaaatgatc gctttcatcg agcggctcaa tcccgagcgg gtcgagatcg
     2221 cccacgcgca atattatggg tgggcgctgg cgaatcgcgc cgccctgatg cccacgcgcg
     2281 cgcaagtaga aacggcgcaa gctgttgtga gcgaagccga aaagcgtctc gcaggaaaga
     2341 ttcgcatcga ctcggttgtc cccgattact atgcgaagta tcctaaggcc tgcatgggag
     2401 gatggggtcg aaagctcatg ctcgtcaacc ctgctggcaa ggtgcttccc tgtcatgccg
     2461 ctgaagtgct tcccggaatg tcattcgaga gcgttcgcga gagaacgtta gagtggatct
     2521 ggaatgagtc ctcttccttt cgtcggttcc gcggcgaaga gtggatgccg gagccctgcc
     2581 acagctgcga acgccggacg gaagatttcg ggggctgtcg atgtcaggct ttcttgctcg
     2641 cgggaaatgc atcagtaact gaccccgtct gctcgctcgc tcctgcccac cgccttgtgg
     2701 aatcagccct caacgaagct aatctgtctg cagctgtcgc acaggcggta tctgcaagtt
     2761 cgtttgttca actgcagaaa cagacgacag attggtggag ttaccggacg aatcctgagt
     2821 agggcctgag gccttcagat ctaaagtttg cagtctgact gagtgcaact ttcccctcac
     2881 cacctcacgc caatcgtgat cgggcgcaag tcagtggtga tgccgccgtt tctcccatgg
     2941 agcaagcggt actcgccata gatttctatg cgggaattga ccttgcgcgc gacacctccg
     3001 ccgaagttct caccaaactc gttctgaacc gaggtaagga agttcccgtt gagaggcagg
     3061 ctgttggcga agcatcggcc ccaccacgtg aagaagccgt tacacgccgc tcccggaatt
     3121 gccgtggaag aatccagctt ccctgagcgg tgatagtagg aggcgccgaa aacgatgtag
     3181 ccgctccaca ccttagttac gggcagattg atgatcggat ccgccataag ggtgtacacg
     3241 tggctgttcg caccgggggc ctgtgcggct tcgagcgctg aagcgcgcag gggaagattg
     3301 tcaaactgaa aatcggcccg aaggccgaag tacttgctga ggttgcgagc cacgccggcg
     3361 gcgccaaccg cgcctccgcc gcgcacgaat tgtctggtcg tgccgctgta ggtggccgcg
     3421 ccgccgccca cgttgaagtt ccagttcttg tattcctggg gcttcttgcg gcgagtcgtc
     3481 tcctcaggcg aagcctcctg gctgctggac tgctgattct ccggttgctg ctgggctgca
     3541 ggttgagccg aggtctgggg cgctgcgctc tgctgggcac ggaccgtagc cgttcccgcc
     3601 aggatcgtca cgagagtgat gggaatcagt acgaccctca atccgggaac cgtcgcgagt
     3661 tttcgaatca ttcattctcc tcccgctaat gggcatacct ggcggaacgc agcgctcgac
     3721 ccacagcctg tcaactgcag gcgactgtaa accagcatgt ccgcgatgct cccgccgaag
     3781 attccactcg cagcaggaag ccaacatatt accaactttg atgctgattc cgcgcgtcca
     3841 gccgcccgcg agaaccgctg cccagttcac ggaaaccggg cgcccacgta tgataagaag
     3901 tcatttctga gcaggaacgt cagcgtgcag gatcgctcct caaaacccgc ggtgaagacg
     3961 accccgcctg gcagggcaga gcccgccgtc tgccgtgtcg ggctgatcgg gttcggaacc
     4021 gttggcagcg ccgttgcccg gattctctgc gaacgcccca acggattctt gcgtctcacc
     4081 cacatctgca atcgcaatgt gcagaggaag aaacagccat gggtccccaa agacgttatc
     4141 tggaccgatg atgtgcaatc ggtgctcaac tccgatattc agattgtcgt ggagctcatt
     4201 ggcgggctca gcccagccga acagatcgtg cgcagtgctt tggaggcagg gaagtctgtg
     4261 gtcactgcga ataagcagct gatcgcacgc catggccctg atcttttgca actcgccgcc
     4321 agccgaggat gccggctcga gttcggcgcc tccgtcgcag gcggggtccc ggttctgccc
     4381 gccctgcgga ccgggctttc gggagaccgt ctgcacggca tcgccgggat cctgaatggt
     4441 acgtgcaact acatcctgag ccggatcgag agcgcgagaa tcccgttcag cgaagcgttg
     4501 gaggaggccc aggcgcgtgg ctatgccgaa gccgatgcct ccgaggatct ggatggcggc
     4561 gatgcccgag ccaagctcac cattctcgcg ctcgccggac tgcacagccg ggtcactcct
     4621 gagacggtac gggcccgcag cattcggccc atcgatgctg tcgattttga ctatgcggcg
     4681 gaactcgact gtaccatccg ccagatttcg cgcgctgatt tgaaagacga cacattgttt
     4741 gccgaggttg gtccatgcct ggtacccgca acctcacctt tcgggcgtgt gcagaaaaac
     4801 ctcaatctgg tgctgacatc ggggcagtac ggcggtgaca cggcgtttct gggcgcgggt
     4861 gccggcgggg atcccacggc agtggcggtc gtttcggact tgatgtctgt ggcggaaagc
     4921 ctttctgcgg gagcaaggaa cgccgtaact tcagtctcag cgcccaacat cagcagtgac
     4981 ttcgagacac cctggtatct gcgcttcttt gtacgcgacc ggccgggaat cgtggcccgg
     5041 ctggcgcgga ttctggccga acatcacctg aacattgatt cggtgctgca aaagccgggc
     5101 ttcgacaagg ctgctctgcc ttttgtcatc acgctggagc cctgccgcga ttcgttgctg
     5161 caccctgcgc tggagaaaat gtcagagctc gattttgcga tccggccgtg tctctgcctg
     5221 ccgattctgc gttaataccc attcgaggga aaacacaaca gcgtcagccg tggcttacgc
     5281 agctgccaag ctgacgctga ttcagtgtcg actagttcca gaagggattt ttcaaaccgg
     5341 cagggcggag cgcgctgtca gtcttgtctt ccgtgtccgt ctcgtccgaa atcttttcgt
     5401 cgagaaagcg cgacggcggt gccgacattt cgttcccctt ctcggtcttg ccgggagaaa
     5461 atggattatt agatttcacg gggcaaagtc ctccatttct aagatctaga cttattaaac
     5521 acgtgcaagc gcgtccccag ggtgcaaaag tttgaatcgg ttccatcgaa aaaccggcat
     5581 gctctctaac tcacaaatcc gcgtcagagg gcagatcagg gcgtgaatcc ggcgttcgag
     5641 atattcttgt ctggtccagt ttggacgtca tttccctaat cgaagtctcc cctgcaaagg
     5701 acgccatcat gaaataccgc aaattcggag gcacgggtat cgaagtcagc gagatggccc
     5761 aaggcctttg gggcatgggc ggttggaccg gttcgagcga tcaggaatca atcgctgcga
     5821 tgcaactcgc ggtcgacctc ggctgcaact tcttcgacac agcctgggcg tacggcgagg
     5881 gcaagagcga cagcctgttg ggcgaaatca tggcgcggaa cagcgggaag cgcctctacg
     5941 ctgcatcgaa gattcctccc gcgaacgacc gctggccggc gcttccggag tacaagtacc
     6001 aggatgtctt ctcggcgaag catgttttca agtacgctga cctgattcgc aagaagttgg
     6061 gcaccgacac gatcgacgtg ctccagtttc acgtctggga tgacagttgg accgacgagc
     6121 ccgaatttcg ctccacggtg gagaggctga agcgcgacaa gtggattcgc tggtttggcc
     6181 tgagcatcaa ccgctgggag ccggaaaact gcatcaaggc gctgcgcacc gggctcgtgg
     6241 acgtcgtgca ggtgatctac aacctcttcg atcagtcccc ggaagacgag ctttttcccg
     6301 tgtgccagga actcaacatc ggcgtcatcg cgcgcgtgcc tctggatgag ggcagcctgg
     6361 gtggcaaaat gacgctcgag acgcgctttc cgaaggacga ctggcgggcg ctgtatttcg
     6421 gaccggagaa ccttgctccg acgatcgagc gcgtggaaaa gctcaagaaa gtggtgccga
     6481 ccgggatgac gcttcccgag atggccatgc gattcgttct ctctcacccc gcagtcacta
     6541 ccaccatccc gggcatgcgc aagctgcaac acgtgcgtca aaacatcgcg gctagcgata
     6601 tgggaccgct cgaccggtcg ttgctcgcgg aattgaagaa gcatcgctgg gaccgcaggc
     6661 cagcccattg gtcggactag aagccaaagc agatgccgca cagcccccgg aatcctacga
     6721 gcgctggtag tagtccgcgc tcggggacta ttctcgcttg cggctgggtc cgccccagtc
     6781 atttcaggca caaaaccgcg gcgaggcagc cttgttcaag agcacgtttc tttccttcct
     6841 gacaatcatc gccactttat ccccgctcta tggccagaac ccccgcggat cgctgcgcgg
     6901 gagcgtgcag gactcgaccg gagcacgcat cccttccgcc ccgatcgtgg cgcagtcggt
     6961 ggattcgtcg ctgcgccgcg aagccaccag cgaggatcgc ggcgaattcc gcatcgacga
     7021 cctgccgccc ggcaactatc acattacggt aaacgcagcg ggcttcgcgc aagccgaggc
     7081 ggacgtcgcc atcgccgtca gtttcgtacg cgaagttacg gtcaccctca taccggcaat
     7141 tccagcagag acggtcagcg tgcaggcggc ttcttcctcg attacaactg aaatcagtga
     7201 tacatcgagc gctgtgcgcg gaggtgtggt cggcagccag gatctgagga ctttgccgct
     7261 gccggcccgg agttttgcca acatcgcgta tcttgtgccc gggaccgagc cagtcgaacc
     7321 gtccgatccc accaaggcgc ggatcacggc ggtctcgacc ggcggaagct ccggcttgaa
     7381 caacgagttg tcggtcgacg gtgccgacaa ctccgacgac tggattggcg ggttcctgca
     7441 aaatttctca cccgatggcg tccaggaatt tgccgtgcgc acatcgaacg aagacgcgga
     7501 cacgggctcg actacggccg gctcggtcgt cattactacc aagcgcggga ccaatgtgtg
     7561 gcacgggagc ggcgcgttct atgaccggca agcagcgctg aatgcgcgtt tccccatcga
     7621 aaatccggcg cccaatccca agcagccctt ctctcgccag aactatgtcg ggacggtcgg
     7681 cggacccatc gcaggcaaca aagtctggat ctttgcatcg ttcgaaaacg tgcacgaaaa
     7741 cgccagcatc gcctacagcc cggcgagcac taagcagttc gacgctctga gggatctggc
     7801 gtcggaagaa tcgaccaccg gcattccgtc gattgctgtg cccgccacgg ttcccattcc
     7861 cttccgcgac tacatcagct cgctccgctt cgactgggcg caatcggaaa aatcgaactg
     7921 gttcttgcgc acgtcgcaag acagctacct gacgcacaac aacctggtcc agcaggccac
     7981 gctgccgtcg accggtttga ccacgcacaa caattactgg aacgtcgcgc tcggcaacac
     8041 ctatgcgttc aattcgaact ggctgggaaa tctggtggtt gacgccagcc tgttgcatct
     8101 cacgcagacg cgaaattcca atctcggatt tgcgatccaa tttcccttca gcgtcacggc
     8161 gctcactgtc tccggcttcg agaccttcgg cgacaatcag ttcgccacgc ccatcactct
     8221 gttccccgat ctgcgcaatc aggataagta ccagttccgc tatgacgtga ctcacgccac
     8281 cggcgaccat tcaatcaagt tcggtgtgga ttttatccac gagccggtgc tgggcggagc
     8341 gttcgcctcc acagcggaag aactcatcca atatccatcg aatccagact gctacatcag
     8401 tccgaccgga gcggactgcg gcggaattac aaatccattg cctttttact tcactccccc
     8461 taatcccgcg actgcttgta ccgggaccat cccaatcggt gtgaattgca ctttcacgcc
     8521 tgtcggcaac gggagctttt cgcagaacgt gcagcgtctt gccttctacg cggaagattc
     8581 gtggcgcgtt tcgcatcagc tcacagtcaa ctatgggttg cggtaccaga cgacctgggg
     8641 gctgttcatt ggctccggac gcagtcaagc agacaacgcc gcctttgcga cgctgcaggc
     8701 gttgcggatt ccggtcgtgc ccagcgtgcc gcaggactac cgcaaacaga tcgcgccccg
     8761 gctcggtgtg gcgtactccc ccggtagcag cggaaatacg gtgattcgcg ccggctttgg
     8821 tctgttctat gacgatctcg cgcagaacgg atggggcacc gcctttcagg cggtcaatgc
     8881 tccgccggtc ccgtgtgtcg atcccgtgca gaatcctggt ggctccgaaa acgccggctg
     8941 cattccgggc agtgccctcg ccggggcggg aaatctgatt ggctcgtcct acaagacgcc
     9001 ttacgcgatt cacgcgaccg gaggcgtgca gcatgccttc aacgaacgct ggctcatcag
     9061 cgccgatttt gtccacgagc agggcaatca cggataccga gctttccctt acgctggcgg
     9121 cgtcaacttg ttgactccgc tttttcccgc tagcgatacg accgatcagc agaacgttgt
     9181 gcccaatgtc aatgtgttcg aatcagataa ccgatccagc tacaacgccc tgatgctgca
     9241 cctgcagggc aacatgcatc gctttaacct agtggcgaac tatacgtttt cgaaggcaca
     9301 gacctgggga tgcctgctgg gcgaactctt cgactatgtg gacggggttt gcccgaatga
     9361 gcccaatacg ccgaatgcag gcttcctgaa tgccttcgga caaggcgact atggcccgtc
     9421 gggcgaagac gtccgccacc gcttcgtcct cgccgggacc ctgcacgtgc cgggcggatt
     9481 cgagttgagc acagttaccc aggtggaaag cgcgcgcccc atcaccattg tgaacgcgaa
     9541 gaactccgat cggatctacg tcaacggcgc ctacaccagc ctcgacgaat ttcgcggcac
     9601 gccctacatt caatcggacc tgcgcgtcac gcgcccgttc aaaatcaacg agcgctggcg
     9661 ggtcgatccc tttatcgagt tcttcaacct cttcaaccgc aacaatcccg gagcgaacta
     9721 tgctgtgaat gtcgcgcaac tgccggtgcc ggcagcccag atgcagcccg atcccgtcac
     9781 cggaatcacc aacgtgacca gcatttgcac ggtcgcggac tgctcccaga ccgttcctat
     9841 tacaagcctg aagcatctgg aaattccaga aggcgctctg ggagattttt tcggcccggg
     9901 aaccaccgtc ggaattccct tcgccgccca gttgggagtg cggctcacgt tctgaaattc
     9961 gtccggattt cagagacaag gtctcagcgc acgagccact cagtcgctgt gtgcacctgt
    10021 accagcaact ggctgtaggt tatggcgagt acggaaaggg tgaccaaggc cgcagccagc
    10081 gggtaaagcg gcgaacgctt gcacggagct tcccgccgct ccgtgctcca ggcaatcagc
    10141 cttcccacgc gtgcattcac cgcagagacc ggattgtgca caagtgccgc agtcaggtcc
    10201 gagggagtct ccggcggtcc aagacgcgca agcttgatca atgctgccgc cagatcgagt
    10261 gcctcgccgg cgtcgctcac cgctgcgtca tcggctgcca tctcgatggc ctcaagccag
    10321 gcggcttcaa ggccgcgcat ggctggaaaa ggcaccaggc gcagtacgag ctttttgaga
    10381 ttgtcctggc gtgccacatg ggcgacctcg tgattcaatg cgatccgtag ctcgcccgcg
    10441 gttaacaaga actccgccgc ctccgatagc aacactttgg gccggcatat tccggcaacg
    10501 gtcattgcgg gaaccgggcg aggaattctt agaaccggaa ccgaggccgg caactcgacc
    10561 agctgcgcgc ctcgcgtgca agctgcaatc ccgtgcgcgg cgcggcgcag ggcagtcgct
    10621 gcggtcactg cacctaagat tcccagcaac actccgcacg aaccgagcgc gagcggaatc
    10681 gtccccagcg gctcgtcgat cgcgcgcggc tccagcagca gaaacgaagg aaccacaagc
    10741 gccagcgtga ccgtggcagc gcccactagc ggaaaaactc gcaaggcaaa cagcgagtct
    10801 gcgagccggc gcgtggcaaa tccgccgatg cgcagcctga tcttgctcca gctcacggcc
    10861 accgccagcg acaaaactcc gtacacgatt acgaacacag agaacgagac ggagataccc
    10921 ctgagggcga acatcaatcg ttttcctttc ttgtttcggc cctgcgtgga tcgcccctcc
    10981 gcagctgacg gcgcttggtc tcgaccaact gaagcaactc ctccagcagg cgggcatcgc
    11041 gctcagtgag gacctccacc aggtgcgaca gcggcagcga tcccggactc gaaccaagca
    11101 gctgacggaa cgcttcgaga gcaacttcgc ggtgcaattc ttcccgggag aatcgcgggc
    11161 tataacggaa agctcgtcct tcagtctggc gcgacagcag gttcttcttg aagagccggt
    11221 cgagcgtggt cataactgtc gtatacgcca ggtcgtgata accgttctgc agaagctcgc
    11281 ggactgtggc gttcccccgc gcccagagcg cctccagaag gcgctcctcg agtggaccaa
    11341 gcccgggcaa ggaatctctg aacccaagaa tgcctttcat gttaggagat aaatcctacc
    11401 ccactgcgaa atccggcgcc ataggaagga cgagagcgtt ccgtgaattc atggcgaccc
    11461 cggagggagt cgaacttgag gcgttttgca ggcgtgagcg aagcgcgagc ctgctgccga
    11521 aatcctgagc ggagcgaagg acctgtgcct gatggcgacc ccggagggag tcgaaccccc
    11581 aaccctcaga tccgaagtct gatgctctat ccagttgagc tacggggccg cgaaagtcac
    11641 accagcaccg gttccagaga ttgggagtgc cacgaagact tggggtggga gacgggaatc
    11701 gaacccgcaa caaccggagc cacagtccgg tgctctgcca gttgagctac tcccaccgcg
    11761 acgttctgat tatagcaact ggggtttctc tctgtcagag gcagcgacgg aaagcagaac
    11821 gacgttgtag gtcgatatgg cgatgaagct acaactcccg gtgggagtcg aagctagacg
    11881 cggccaacgc cgacgtattc aaaccccagc gcgcgcagac gggtcgaatc gagcaggttc
    11941 ttcgtgtcaa cgatgaccgg gtttcttagc aagtgcttga tgcggtcgaa gtcgagtgcc
    12001 cggaactctg gccagccggt gccgaccacg agtgcatccg agccttcggc agcggcgtag
    12061 gcagaatcgc agtagcgtac cgatccattg agccgagact tcgcttccgg aatggccact
    12121 ggatcgtaag cccggacatg cgcgcccttg gagaccagcg tctcggcgag gcgcaacgac
    12181 gacgacccgg ccactgaatt ggtgtttggc ttgaaagcca agcccagaat gccgacttcc
    12241 ttgttctcga cggagctcag cgcctgcgtg attttctcaa ccagccgctc ggcaagcagg
    12301 tggttcacgt cgcgcgcggc gttcaggact ttcagcgaca cgccatgcga ctgcgcgagc
    12361 tcgaccagcg agtccatatc ggactcggcg aacacgcctc ccatgcccgc tcccggctgc
    12421 aaacaacgag gagcaatttt cttgtccagg ccgagggcaa gggcgaggtt cacggcatcg
    12481 gcgttgacgc gctcgcagag cgcggcgact tcattgatga aggagatctt tgtcgcaacg
    12541 aaggcggtag cggcttcccg caccagttcg gccgtcgacg cgttcgtgac gatgacaggc
    12601 acgccgcgca taaccagcgg atgaaaaatc tgtttcagca cctgcactgc ctcattcgag
    12661 cttgttccca ggatcagccg atccggccag ttgaagtctt ccactgcgca cccgtcggtg
    12721 aaaaacatcg gctgggtgac aatggtcgcc tgtaaccccg cgtctttgat gtttttctcc
    12781 aacgcggtcg cggtacctac cggcgcgggc gtgacgatcg tcagaatcgg aggtttggag
    12841 gcgagccgcg caatgcgcat ggctacatct ttcagatgct cgaccttgtc ttcagcgaga
    12901 aaaattacct gcgtcttgcg cgcgaaggac tcgatgtcgg tcgagtaagc cagtcgtcct
    12961 gcgcgtacgt tgcgtcgaat gatctctttc agattctttt caaagaaggg aatattgcct
    13021 tgagccatct cgaccatgcg ggagcggtca ggatggcagc aggaaaccgg ggttccaaaa
    13081 tctgcgagac aggccgaaat cactgttccc aggtatcccg aaccgtaaac cccgatctgc
    13141 atgcgctttc tcccgttgct tggcggaaac ccgcgtcaga gtgggcttcc aggggcggaa
    13201 tcccgacact cgccctgtcc tttatctacc gccccggcag agccttttca acgcacggga
    13261 ggatgatgta cctagggcca tgtacaacca agactagtaa ccttgtcggg gattactgcg
    13321 ggctagcggg agacgagagt gcgtccgagg ggaaccggcg ccacatcgac ccagcaattc
    13381 tgggtgtcct cacggacgaa tgtcagcttt tccagcaaga tacgcctgct gccagagtac
    13441 cggagccaac gttcgcgagc gatcccgaag gcattctgcg cgacctgctc cccgctcatt
    13501 ttcacgatgg tgaggtgcgg aatgtacggc cattcttcct gagacgcgag tgcctcagca
    13561 ttaagccgct ggtgcaatgc gcacatgcgc gaagccgccc cgtcgacacg aatgtacaca
    13621 gtgggcgtcg ccgggatgaa ggtttccact ccgcccaggt gcacttcaaa gggctcctct
    13681 tggccgcaaa ttcgctccag gagttgcaga gccgaatttt cggagccttg cagagagcgt
    13741 gggggaagga tcgtgagatg tgccgccaga tggggttgtt ccggatgcag ctcccgccgc
    13801 aggctttcta caaactctcc caccggccca cgaacatagg caaccagggc gtaacgcgaa
    13861 ttctgcatga tcgcactgcg attatactcc gagggccgca acggccctgc cccctaaacg
    13921 gatcagcggc gcaggccacg ttcagcgact gcgagcgcgg tggtgcggtt cagcaaaagt
    13981 aatttagaat cagtacgagt cggggcgtag cgcagcctgg tagcgcacct gccttgggcg
    14041 cagggggtcg taggttcaaa tcctatcgcc ccgaccaaca actctactcg tactcgacag
    14101 ggtcgcggac gaacctgggg gttcccggct ggcctctcgc cgcaacgctg gggacgcagt
    14161 tccgtcgagg ttatctgtct atggcgcttc acgggaagcc gtcgggaggt cgcgtgcttt
    14221 ctctgcttgt ttcttgcttt ctcctcctcg gcactgcctc gaacaactcg cttcctgagg
    14281 ttcggtaccc tgagccggat atcggtgtgg actcgcttgc cgcgaggaaa aaggcacagt
    14341 tggagacggt gggtcagttc aaagtcttct atcagtttca tctcaccgat caagtgaagg
    14401 agagcggtat cacatttgtg aaccacgtcg tggacgatgc cggcatcgac tacaaaccag
    14461 tgcactacga tcacggcaac ggaattgccg tggcagacgt agatggcgac gggctgtacg
    14521 acatttattt cgtaaaccag gtcggaggga atgagctgtg gaagaatctg ggaggcggca
    14581 agttcaagaa cattaccaaa gaggctggag tcgggctgcc ggggcggatc agcgtcagcg
    14641 cgtcgttcgc ggacatcgat aacgacggcg cccaggattt gtttgttacg accgtgcttg
    14701 gtggcaatgt gctgttcaag aatgacgggc acggacactt ccgggacatc acgaaagagg
    14761 cgggcctcga tctcgtgagc cattcttccg gcgcagtttt cttcgattac aacaacgacg
    14821 gcttgctcga cctgctcgtt tgcaacgtgg gcaagtacac gagcgacaaa aaaggcgcgc
    14881 agggtcagta tgtcgggctg ccggacgctt tcggcgggca cttgcatcca cagcgatttg
    14941 agtatccggt cctctacaaa aatcttggcc acgaccgctt caaggatgag actgccgcgg
    15001 ttggcctgcg gccgacagga tggtgcggcg acgcaacctt caccgacccc gacggcaagg
    15061 gcttccccag cctgttcatt ctcaacatgc agggccacga ccactacctg gaaaacgagg
    15121 gcggcaagag cttcgtggac aagaccgaac aatattttcc caagacacca tggggggcga
    15181 tgggagtgaa gttctttgat ttcgacaacg acgggcgcat ggatctgatc gtcaccgaca
    15241 tgcactcgga catgagcgaa tcggtggggg tagagcggga aaagctgaaa tcaatcatgc
    15301 gctggcccga ttcgttcacg caggccgcct cgcacgaaga cttcatatgg ggtaatgcct
    15361 tttatcaccg cctggagaac gggcagtacg aggagatctc ggaccggatc ggagtggaga
    15421 actattggcc gtggggcgtg agcgtgggcg acatcaacgc cgacgggtgg gatgacatct
    15481 ttatcgcgtc gggcatgagc tttccgtttc gctacggaat caactcgatg ttgctaaaca
    15541 accggggaga aagattcctg gattcagaat ttctgctggg aattgagcca cgcaaaaata
    15601 tttatgtccc gtggtttgac ctggactgct cgcaaaagaa tctgactgac ctgaaccaga
    15661 aagtctgcgc cgggcggtcc ggcaagattc gcgtcctgtc gccgcgttca agccggtcct
    15721 cggtgatgtt cgatctcgat aacgacggag acctcgacat cgtcaccaac gaattcaatt
    15781 cggcgccgca ggtgctgatc agcgacctgg cgcagcagaa ggcgatccac tggctcaagg
    15841 tcgttctggt tggcactcga tccaatcgta acggattcgg cgcgacagtc cgcctgcaag
    15901 ccggcggaca gacctatacg aaatacaacg acggaaaatc tggttatttg tcgcagagcg
    15961 tgttgcccct ttacttcggc ttgggcgacg cggagaaaat cgacaaggtt gaagtggatt
    16021 ggccatccgg ccagaaacag gtggtaacca ccgggcttgg cgaaaaccag gtcctccgca
    16081 ttacggagtc gaaataacgc accctgtgcc cctccacccg gctcggtcca agaccaacaa
    16141 aaactgctgg acttggccga aaaccggaat acgatatagg tccatgaagg cttcgtagag
    16201 cggatggatg cccctgacta gaccgctgct ctggggagga ggctatgcaa caagtcgggt
    16261 ctctgatccg cttcgccagc gagctggtga gatcgtcatg cgctggcgcg ttacttttca
    16321 gcgcagtgtg tcttttcgcc cagcatggag gtggcggcgc gcgtccgcaa tcacctccac
    16381 cccaggctcc cacggcaccg ccatccgcga ccgatttcga acagaaccga ctctttctcg
    16441 agagcaacgg caagccagtc tccgccaaga acccgcaggg agagagctgc ttgcttcccc
    16501 cgcttagtag cgttgtgcct gcc
//
LOCUS       DASPUL010000067         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1928387, whole genome shotgun sequence.
ACCESSION   DASPUL010000067 DASPUL010000000
VERSION     DASPUL010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928387"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            456..>3177
                     /locus_tag="VEJ00_01670"
     CDS             456..>3177
                     /locus_tag="VEJ00_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXW99892.1"
                     /translation="MIRLELAPKTDSSPLHAGAPHSSTQNDRWGLAVTSSARPFAVDE
                     QVGDYKILGTAGVGGMGIVYKALDLKLNRTVALKFLPNDLNLNSKDKERFLQEARTAS
                     GLDHTNIGVIHGLEQTSDGHTFIVMAYYEGLTLAERIRRGPIAPKEALGIMVQLAHGL
                     IEAHEHSIIHRDVKPSNVIITRQGVAKIVDFGLARVAGGAATTLSAGPVGTLAYMSPE
                     QVRSEPLDRRTDIWSLGVILQEMLTGKHPFERDNMSAVLLAILEQPPPVEGLDPALAA
                     IVCRALAKDRVHRYNDCREMLADIEACKPVFDAHPTAPVPRDSRSSALNLRKALGHAS
                     STRPLATPMDRAWRWAVELIAAILVVIVLTLLIPSARQRVATLLSRGGGEEKHIAVLP
                     FDNIGHDPANEEISEGLMDSLTSKLSNLESSENGGQQSLWVVPASEVRRKKVEDPEAA
                     LREFGATLVVKGSVMRDASGVRLTVNLIRTKDVRQEGSLSLQNQSGDFAALQDQAVSG
                     LARLMHVEVPATALRAAEGPAAPAAYESYLKALGYMQRYDKPGNLDRAIASLTSAVES
                     DAQFALGYAGMAEAYRLKYQLDKNPRWTDMALADGNKATALNDSLPSAYVTLGRIHDA
                     LGKNDLALAEFDRALKLDSRNADALTGEAHSYEHAGRLADAEAAYKKAIALRPDYWDG
                     YNSLGLFYDDQGRYDDAVKELQHAIELTPDNATAYSNLGAAYVDSSDPKNYPQAEAAL
                     KKSLELAPSYGAYANLGDLYLLQNRYAESAGVTEKALQMNDKDFIVWANLVAAYDGLK
                     DEAKANTVVDREQVLLEQAAKSSPRDAAVQYRLGIVYARKKQGEKALACAQTALALSP
                     DDAYTLTSVAEIYERLGDRKRALQYVEQGLQKGYPLQALQNDQFFKNLLAD"
BASE COUNT          638 a          916 c         1053 g          570 t
ORIGIN      
        1 gcggcggagt ttgcccggaa gttgggggta aagaatgcgg cgctgtcgct ttcgcataca
       61 gcggagcagg cgatcgcgca ggtgattctg gagggatagt cgacctgacg tggaaagtag
      121 agattccagc ctgtcaaaaa cgaccacaag ggctaaagcc cggtcggggt cgatgctgaa
      181 cgcggcgctg aagcaccgct cttccacggt gctccaaaca ccatctcaac catttggcca
      241 tttccacacg gtggtcgagt caccgtctcg acgatcttcc accaaacacg ctgtggcgtc
      301 atcccgacgc cccgggatcg aattgtcagc gccgcgattt tcgcctgggg catcgctcgc
      361 tgttgttatt ggaacccggt tccacaatgg gcccgaaagc ggcgcggggc ctgagattcg
      421 ccctagctgg cttgcaacgg ctgagtggcg gcgctatgat tcgcctcgaa ctggcgccga
      481 agacagattc ctctccgctc catgccggag cgccgcactc ctcgacgcag aacgatcgct
      541 gggggctggc agtgacatct tccgcgcggc ctttcgccgt tgacgagcag gtcggtgact
      601 acaagattct gggcacggcc ggcgtcgggg ggatgggcat tgtctacaag gccctcgacc
      661 tcaagctgaa ccgcacggtt gccctcaagt tcctgcctaa cgatctgaac ctcaattcca
      721 aagacaagga gcgcttcctg caggaagcgc gaactgcctc agggctcgac cacacgaaca
      781 tcggcgtaat ccacgggctg gaacagacct cggatggcca cacgttcatt gtgatggcct
      841 actacgaggg cctgacgctg gcggagcgga tccggcgcgg gccgatcgcc ccgaaagaag
      901 cgctggggat catggtgcag ctggcgcacg ggctgatcga ggcgcatgag cacagcatta
      961 ttcatcgcga cgtcaagccg tcgaacgtga tcatcacgcg gcagggcgtg gcgaagatcg
     1021 tggacttcgg gctggcgcgc gtggccggag gcgcggcaac gactttgagc gcgggaccgg
     1081 taggaacgct ggcgtatatg tctcccgagc aggtgcgctc ggagccgctc gaccggcgga
     1141 ccgacatctg gtctctgggc gtgattctgc aggagatgct gacgggaaag catccctttg
     1201 agcgcgacaa catgtcggcg gtgctgctgg cgattctgga gcagccaccg ccggtcgagg
     1261 gactcgaccc ggcgctggcg gcgattgtgt gccgggcgct ggccaaggat cgcgtgcatc
     1321 gctacaacga ctgccgcgag atgctcgccg atatcgaggc gtgcaagccg gtgtttgacg
     1381 ctcatcctac cgcaccggta ccccgtgaca gccgaagctc cgcgctcaat ctccggaagg
     1441 cgctggggca cgcctcgtcg acccggccgc tggctacgcc gatggatcga gcctggcgct
     1501 gggccgtgga gctgattgcg gcgattctgg tggtgattgt cctaacgctg ctgattccct
     1561 cagcgcggca gcgggtcgca accctgctgt cgcgcggcgg gggagaagag aaacacattg
     1621 ccgttctccc gttcgataac atcggccacg atccggcgaa cgaggagatt tctgaagggt
     1681 tgatggacag cctgaccagc aagctgtcga atctcgagag ttccgagaac ggcgggcagc
     1741 aatcgctctg ggtggtgccc gccagcgaag tacggcgaaa gaaggtggag gatccggagg
     1801 cggcgctgcg cgagtttggg gcaacgctcg tcgtgaaagg cagcgtgatg cgtgacgcct
     1861 cgggcgtgcg cctgacggtg aatctgattc gcaccaagga tgtgcggcag gaggggtctt
     1921 tatctttgca gaaccagtct ggcgattttg ccgcgctgca ggatcaggct gtttcggggc
     1981 tggcgcgact catgcacgtg gaagttccag ctacggcgct gcgagcggcg gaagggccgg
     2041 cggcgccggc ggcgtatgag tcctatctga aggcgctcgg ttacatgcag cgctacgaca
     2101 agcccgggaa tctggatcgg gcgattgcgt cgctcacgag cgcggtggaa tctgacgcgc
     2161 agtttgccct cggatatgcc gggatggccg aggcgtaccg tctgaagtat cagttggaca
     2221 aaaatccccg gtggaccgac atggcgctgg ccgacggcaa caaggcgacc gcgctgaacg
     2281 attcgctgcc gtcggcatac gtgacgctgg ggcggattca tgacgcgctg ggcaagaacg
     2341 atctggcgct ggcggagttc gaccgcgcgt tgaaactcga ttcgcgcaac gccgacgccc
     2401 tgaccggcga ggcacactcc tacgagcacg ccgggcgtct tgccgatgcc gaagctgctt
     2461 acaagaaggc aatcgcgctc cgcccggact actgggacgg atataacagc ctcggacttt
     2521 tctatgacga ccagggccga tacgacgacg cggtcaaaga attgcagcat gcgatcgaac
     2581 taacccccga caatgcaact gcgtattcga accttggggc agcctatgtg gattcgagcg
     2641 atcccaagaa ctatccgcaa gccgaagcgg cactcaagaa gtctcttgaa cttgcgccca
     2701 gttatggcgc gtatgccaac ctcggagatc tgtatctcct gcagaaccgc tatgcggaat
     2761 cggcaggggt gaccgagaag gcgctgcaaa tgaacgacaa ggacttcatt gtctgggcga
     2821 atcttgtggc agcgtacgat gggttgaagg atgaggccaa agcgaacaca gttgtcgacc
     2881 gggagcaggt ccttctggag caagcggcga aatccagccc gcgagacgcg gcggtgcagt
     2941 atcggctggg cattgtgtac gcgagaaaga agcagggtga aaaggctctg gcatgtgctc
     3001 aaaccgcgct ggcgctttcc ccggacgacg cctacaccct gacctcggtg gccgagattt
     3061 atgagagact aggggaccgc aagcgcgccc tgcaatacgt cgagcagggt ttgcagaagg
     3121 ggtatcccct gcaggcgctg cagaatgacc agtttttcaa gaatctgtta gcggatc
//
LOCUS       DASPUL010000068         3831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_283991, whole genome shotgun sequence.
ACCESSION   DASPUL010000068 DASPUL010000000
VERSION     DASPUL010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283991"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VEJ00_01675"
     CDS             complement(<1..978)
                     /locus_tag="VEJ00_01675"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXW99893.1"
                     /translation="MKRISSLVFVLLLITQALIAQDAKPEKAPDSERHEFVIANFKTE
                     SGVTLPQARIEYGTYGHLDAAKDNAILLPSHYMADFHGYEWLIGPDKALDPAKYFLVA
                     TELFGNGHSSSPSNTPEPLHGPRFPVTTIRDNVEAVHRLLTEELKITHLRAIIGFSMG
                     AQQAFQWAVSYPQFADRIVATCGTAKTWPHGFVRLEGQIAALTADAAFQNGDYTSQPK
                     KGLEAFGVVWTAWLFSQEWWRKELWREGAPPDTTFEKVLYDFRTNFIPGADANDLILQ
                     MRTWEKHDVGTTPGFAGDVAKALGSIKVPFLYMPGETDLYFPVGDARYEA"
     gene            1391..2404
                     /locus_tag="VEJ00_01680"
     CDS             1391..2404
                     /locus_tag="VEJ00_01680"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HXW99894.1"
                     /translation="MSTASGSKPIRAKISALGTYVPPRLLTNADLEKMVDTSNDWIMA
                     RVGIRERHIVDKGVATSDLAVEAAKRALAQRGLTPNDIEAIIVGTVTPDMFFPSTACI
                     VQHKLGAKGIWGFDLSGACSAFVYSLQAGAQFIASGAHKRVMVIGSDVMSSIIDYTDR
                     ATCVIFGDGAGAVILEPAEDESVGLIDFIHEVDGSGGQYLYMPGGGSLNPSSKETVER
                     KMHYVHQDGQQVFKFAVRKQTEACEKLLARNGLKGSDIDAFIPHQANLRIISATADRL
                     GLRPEAVIVNIDRYGNTTAGTIPLAMGTAVEEGKLKKGSLVLLASVGAGFTVGATLLR
                     WAF"
     gene            2553..2993
                     /locus_tag="VEJ00_01685"
     CDS             2553..2993
                     /locus_tag="VEJ00_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXW99895.1"
                     /translation="MRHTIFCCLLLTLVTIGMAAQQADPREGKILVLERLWNEAQVNR
                     DAPALDAMVAERFVNTEYDGEVSDKTKFLSDIRDPQFKPSSATIQDLKVTFFGDTAIV
                     IGTYHTKGTYQGKPYDHFGRFTDTWIQQLGKWQCVASHTSLVKK"
     gene            3173..>3831
                     /locus_tag="VEJ00_01690"
     CDS             3173..>3831
                     /locus_tag="VEJ00_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXW99896.1"
                     /translation="METTQPRAWKVLLAFAMIYFVWGSTYLAIRVGVREVPPFLLAGI
                     RFLIAGVVLFVWMRFKGTASPTLREWGGVTAMGLLIFVFDYGLVFWAEKRVPSGIAAV
                     MMATIPVFMSLAEIVVLRTQRLTARLGIALLVGLAGVAILVGHAMSFGEDPIDTAGGC
                     ALIVGAISWSVASIISRKLPLPADKVMSSGAQMLAGGVLLTLTAASLGEFRGFHVQAV
                     SR"
BASE COUNT          746 a         1110 c         1185 g          790 t
ORIGIN      
        1 ggcctcgtag cgcgcgtcgc cgacggggaa atacaggtcg gtttcccctg gcatatagag
       61 gaacggaacc ttgatcgaac cgagcgcctt cgcgacatcg ccggcgaatc cgggcgtggt
      121 tccgacgtcg tgcttttccc atgtgcgcat ctgaagaatt aagtcgttgg cgtcggctcc
      181 agggatgaag ttggtgcgga aatcgtacag tactttttcg aatgtcgtgt cgggaggcgc
      241 tccctcgcgc catagttctt tgcgccacca ttcctgggag aacagccatg cggtccacac
      301 gaccccaaag gcttccaggc ctttcttggg ttgtgaagtg tagtccccgt tctggaaggc
      361 ggcgtcggcg gtgagcgccg cgatctgccc ttcgagccgg acgaatccgt gcggccaagt
      421 cttggcggtg ccgcaggtgg ccacaatgcg atcggcgaat tgcggatagc tgaccgccca
      481 ctgaaacgcc tgctgcgcgc ccatagagaa gccgatgatg gcgcgcaggt gcgtgatctt
      541 cagttcttcg gtgagcaggc gatgaaccgc ttccacattg tcgcggatcg tggtgaccgg
      601 gaagcgcggg ccatgcagtg gctcaggcgt attgctgggc gacgacgaat gcccgttgcc
      661 gaacagttcg gtcgctacga gaaaatattt cgcgggatcg agcgccttat ccgggccaat
      721 cagccattcg tatccgtgga agtcagccat gtagtgcgaa ggcagcagga tcgcgttgtc
      781 tttggcggcg tccagatggc cgtaagttcc atattcgatt cgagcctgcg ggagggtgac
      841 gccgctctcg gtcttgaagt tcgcgatcac gaactcgtgg cgctcggagt cgggcgcttt
      901 ctcgggcttg gcgtcctgcg cgatcagggc ttgcgtgatg agaagaagaa cgaagacaag
      961 ggaggagatt ctcttcattg tgtcaggtcc tcaggatggc gggctgtatc ttatcacccg
     1021 gagtcttcgg agtcggtctt cctggcgcgc ttcgctggcg tttccgcgtg aagttcgcgc
     1081 aagaaacctt aaatcgcaaa gaacggagaa aaggccgcag agaccgcgaa gaaaaatgag
     1141 atcgcgttca ccggctcatc ggaaggcgag agcctacatc gactcgtggc cctccagcgt
     1201 cccggtctgg accgggttgg tttgcgctct ttccggcatt tccttggcga actccgcggt
     1261 aagaagctct tgaacgcaag gatcgcgaag ccgcagagga cgcgaagaag agccagtcgg
     1321 gttagacttt cttcttccat tccttcaaaa tactgacttt catactcaag gaagatccca
     1381 ggagccctca ttgtccacag cgagcgggag caaaccgatc cgcgcgaaaa tcagcgccct
     1441 cggcacttac gtgccgcctc gtcttctcac caacgccgac ctcgaaaaaa tggtcgatac
     1501 ctcaaacgat tggatcatgg cgcgggtggg catccgcgag cggcacatcg tcgacaaggg
     1561 cgtggctacc agcgaccttg ccgtggaagc cgccaagcgg gccctggctc agcgcggact
     1621 gactcccaac gatatcgagg ccatcatcgt gggcaccgta actcccgaca tgttctttcc
     1681 atcgacggcc tgtatcgtgc agcacaaact cggcgccaaa ggcatttggg gattcgatct
     1741 ctcgggggca tgttcggcat ttgtttattc tctgcaagcg ggggcgcagt ttatcgccag
     1801 cggcgcgcac aagcgggtga tggtgatcgg ttccgacgtg atgtcgtcga ttatcgacta
     1861 cacggatcgc gccacctgcg tgatttttgg cgacggcgcg ggcgcggtga ttctcgagcc
     1921 ggctgaggac gagtcggtcg ggctcatcga ctttatccac gaagtcgacg gctccggggg
     1981 gcagtatctc tacatgccgg gcggcggcag cctgaatccg tcgagcaagg agacggtcga
     2041 gagaaaaatg cactacgtcc atcaggacgg gcagcaggtg ttcaagttcg ccgtacgcaa
     2101 gcagaccgaa gcctgcgaga agctgctagc acgaaacggg ctgaagggct cggacatcga
     2161 cgctttcatt ccgcaccagg cgaatctccg catcatcagc gccacggccg accggctggg
     2221 actgcgcccg gaggccgtca tcgtcaacat cgaccgctac ggcaatacca ccgctggaac
     2281 gattccgctg gccatgggca cggccgttga agagggcaag ctgaagaagg gaagcctggt
     2341 gctgctggcc tcggtgggag caggattcac ggtgggagcg accttgctgc gctgggcgtt
     2401 ctaaggacgg ggtgatccaa tcccaggacg gacccgccgc cgtgacgttc gtaaccgcct
     2461 ccgacccatt tctctcgcaa cgactacgcg caaactgcta tgttctcgca gcgtccgccc
     2521 tcacgtcggg atgacggcgg acatgtgcga ccatgaggca cactatcttc tgctgtctgc
     2581 tgctgaccct cgtcacgatc ggaatggccg cccagcaggc cgatccgcgg gaagggaaga
     2641 ttctggttct ggagcgcctg tggaatgagg cccaggtgaa tcgcgacgcg cctgcgctgg
     2701 atgccatggt ggccgaacgc ttcgtcaaca ccgagtacga cggcgaagtc agcgacaaga
     2761 ccaagtttct gtccgacatc cgggacccgc agttcaaacc ctccagcgct acgattcagg
     2821 acctcaaagt cacgttcttc ggcgacactg cgatcgtcat cggtacctac cacaccaaag
     2881 gaacctatca gggcaagccc tacgaccatt tcggccgctt taccgacacc tggattcagc
     2941 agctcggaaa gtggcagtgc gtcgccagcc acaccagcct ggtgaaaaag tagttctttc
     3001 tcgcttcatc acagcgacaa ctacagtggt agtctcgaaa tgtttttctc gggagccatc
     3061 ggccaaatgg ccttatcaat tcttcttata tctctttatg atcgaaagag aaccgcagct
     3121 gcgggaggtg atctaaccca ctgcggataa agtcgccgct gcaagcatcc caatggaaac
     3181 gacacagcca cgcgcgtgga aggtcctgct cgccttcgcc atgatctatt tcgtctgggg
     3241 ctcgacgtac ctggcgatcc gagtcggggt gcgggaagtg ccaccgtttc tgctggccgg
     3301 tatccgcttc ctcatcgctg gtgtcgtctt gttcgtctgg atgcggttca agggcacagc
     3361 ctccccgacg ctacgggagt ggggaggcgt gaccgcgatg ggccttctga tttttgtgtt
     3421 cgactacggc ctggtgttct gggccgagaa acgagtgccg tccggcatcg ccgccgtcat
     3481 gatggcaacg attccggttt tcatgagtct ggcggagatc gtcgtgctgc gaactcagag
     3541 gctcactgct cggctgggga tcgctctgct ggtaggcctg gcgggcgtag caatccttgt
     3601 gggacatgcg atgagttttg gcgaggaccc gatcgatacg gctggcggtt gcgcgctgat
     3661 cgttggggcg atcagttggt ccgtcgcgtc gatcatcagc cgcaaactgc cgttgcccgc
     3721 tgacaaggtg atgagttccg gagcgcagat gctcgccggc ggcgttctgc tcacactcac
     3781 tgcggcttcg ctgggcgagt tccgcggctt tcacgtacag gccgtttccc g
//
LOCUS       DASPUL010000069         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1380948, whole genome shotgun sequence.
ACCESSION   DASPUL010000069 DASPUL010000000
VERSION     DASPUL010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380948"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VEJ00_01695"
     CDS             complement(<1..768)
                     /locus_tag="VEJ00_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXW99897.1"
                     /translation="MIQIKDVTKLYPAKEAGNGSGKDNGAIHALDHISLHVTKGEWLS
                     IMGPSGSGKSTLVNLIGCLDRPTAGEIWLDGQNVAGISSADLNRVRAEKIGFIFQQFH
                     LIPFLTAVENVMLAQYFHSMTDEKEAIEALARVGLGDRAHHLPSQLSGGEQQRVCIAR
                     ALINDPRIILADEPTGNLDAQNEEIVLRLLREFHQQGRTIVMVTHDPVVARLADRRIE
                     LHHGKIAAQEVFSMADEEQLDEVLEELWVLHEHGEIAE"
     gene            complement(770..1951)
                     /locus_tag="VEJ00_01700"
     CDS             complement(770..1951)
                     /locus_tag="VEJ00_01700"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW99898.1"
                     /translation="MSHSAQDLAASRAKAPHTSMFLRMLVRAAILRRGRAVSALFAMV
                     VAAAVATAMLNLYVDVQAKLQHEFRNYGANIILVGKDGASLPTDSLSHVDSLLAGRGT
                     AAPFAMIVARTGDGQPIVVAGTDFERVRQLDRWWSVSHWPSQVPASRAQDAREMGHPA
                     LVGVRALPVVSPRNQAFDLSFQGHLLHLTPTGTVQTGAAEDSRIYISLVDFVHWSGVQ
                     PSTIEVAANGSPEEVSGIMNALKQAIPAADVRPVRQIMEGEARVLGKTRATLLAAAAL
                     IILTAALCVLSTLMGWVFDRRRDFAIMKALGASTRLLNGFFAAEAAALGATGALIGFA
                     IGIGLAAWIGRVNFHAPVVPRFSVLPTVLAGSMAVTLLSAILPISLLRRVQPAVILRG
                     E"
BASE COUNT          410 a          630 c          652 g          365 t
ORIGIN      
        1 ttcagcgatt tccccgtgct cgtgaagaac ccagagctcc tccagaacct cgtcaagttg
       61 ctcctcgtcg gccatggaga atacttcctg cgcggcgatc ttgccgtggt gcaactcgat
      121 acggcggtcg gccaggcgcg cgaccacggg atcgtgcgtg accatcacga tagtgcgtcc
      181 ttgctgatgg aactctcgca gaaggcgcag gacgatttct tcgttctgtg cgtcgagatt
      241 gccggtcggc tcgtcggcca gaatgatgcg gggatcgttg atcagcgcgc gcgcgatgca
      301 cacgcgctgc tgttcaccgc cggaaagctg cgagggcaga tgatgcgcgc gatcgccgag
      361 tccgacgcgc gcaagggctt cgatggcttc cttctcgtcg gtcatgctgt ggaagtactg
      421 cgccagcatg acgttctcga cagcggttaa gaaaggaatc aaatggaatt gctggaagat
      481 gaatccgatt ttctcggcgc gcacgcggtt cagatcagcc gaggaaatcc cggcgacgtt
      541 ttgtccatcg agccagatct cgcccgcggt tggccggtcg aggcagccga tcagattgac
      601 cagagtcgat ttccccgagc ccgagggtcc catgatggag agccactcac ccttggtgac
      661 gtgcagggag atgtgatcga gcgcgtgaat cgcgccgttg tctttcccgc tcccgttgcc
      721 ggcttccttg gcggggtaga gcttggtcac gtctttgatc tgaatcattt cactctcccc
      781 tcaggatcac cgccggctgg acgcgccgca gcagcgagat cgggaggatc gccgacagca
      841 gcgtcactgc catgctacca gccagaaccg tcggcagcac gctgaaacgc ggcaccacgg
      901 gagcatgaaa gttaacccgg ccgatccagg ccgcgaggcc aatgccgatc gcaaatccga
      961 tgagagcgcc ggtggcgccc agcgcggcgg cctcggcggc aaagaacccg ttgagcaggc
     1021 gggtcgaagc gcccagcgcc ttcatgatgg cgaaatcgcg gcgccggtcg aacacccatc
     1081 ccataagagt ggaaagaacg cacaatgcgg cggtcagaat aatcagcgcc gcggccgcca
     1141 gcagcgtggc gcgcgtcttg ccgaggacac gggcctcgcc ctccatgatc tgccgtacag
     1201 ggcgaacatc ggcggcggga atggcttgct tcagtgcatt cataatgccg gacacttctt
     1261 cgggagatcc gttggcggcg acttcgattg tggagggctg aactccggac caatgcacga
     1321 aatccaccaa cgagatgtaa atgcggctgt cttccgcggc gccggtctgc accgtgcctg
     1381 tgggggtgag atgcagcaga tgtccctgaa agctgagatc gaaggcttgg ttcctgggcg
     1441 aaaccaccgg cagagcgcgt acgccgacca gagcagggtg ccccatttct cgcgcgtctt
     1501 gtgcgcgaga agcgggaact tgcgacggcc agtggctcac ggaccaccag cgatcgagtt
     1561 gccgcacgcg ctcgaaatcc gtgcccgcaa ccacgatcgg ctgaccatcg cccgtgcggg
     1621 caacgatcat ggcaaatggc gcagcagtac cgcgtccggc aagaagcgaa tcgacgtgcg
     1681 acagactatc cgtgggcagg gaagcgccgt ctttgccgac caggatgatg ttcgcgccat
     1741 agttgcggaa ctcgtgctgc agcttggctt gcacgtcaac gtagagattc agcattgcgg
     1801 tggcgactgc ggccgccacc accatggcaa acagggcgga caccgcgcgt ccacgtcgca
     1861 ggatcgccgc gcgcaccagc atgcgcagaa acatcgacgt atgtggtgcc ttggcacggc
     1921 tggccgcgag atcctgtgcc gaatgagaca tattctgcga tccaggcgtg ctcacagttc
     1981 acctcgcaat gcgaatacgg gatcgagccc gacggctcgc cgaatcgcgg ccgcgcttcc
     2041 ggcaaaggtc acaatca
//
LOCUS       DASPUL010000070         4038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_695489, whole genome shotgun sequence.
ACCESSION   DASPUL010000070 DASPUL010000000
VERSION     DASPUL010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695489"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..426
                     /locus_tag="VEJ00_01705"
     CDS             262..426
                     /locus_tag="VEJ00_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99899.1"
                     /translation="MSTLVRMLWTEDAGQDIAEYAVMLAVILVLVVGTVRLIGSNANN
                     AFSQVASSIQ"
     gene            665..1687
                     /gene="aroF"
                     /locus_tag="VEJ00_01710"
     CDS             665..1687
                     /gene="aroF"
                     /locus_tag="VEJ00_01710"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXW99900.1"
                     /translation="MIVNMSEKATEQEINHVIERIREAGYQPHVTRGTERTIVAAVGS
                     GRRHEIEALQVAPGVDNVVPIAQPFKLVSRQVRPERSVVKVDGVAIGGPEVVVIAGPC
                     SVESREQLFDTALAVKRAGAAMLRGGAYKPRTSPYDFQGLGLEALKILREAKERTGLP
                     VVTEVMSTEDVDVISEYVDMLQVGARNMQNFALLRRLATAKKPILLKRGPSATVKEWL
                     LAAEYLLTGGNSQVVLCERGIRTFETETRNTIDLAAVALARDLSHLPVIADPSHGTGK
                     QSLIAAVSRAAVAVGADGLIIEVHPCPERALSDGAQSLDFAGFARVMQALKEPLRRTS
                     GLPAVS"
     gene            complement(1714..>4038)
                     /locus_tag="VEJ00_01715"
     CDS             complement(1714..>4038)
                     /locus_tag="VEJ00_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HXW99901.1"
                     /translation="PVAGASYWLEDIDVNGVRTLHGPVSLETSMQTGSEVSAAETHMF
                     SQLAQVKVETTDSAESHALEAVRLQSEPTPSQLQKQFELAAKPAVKIWVKREGWYRVP
                     QPALLKAGLDPNVDPAKLHLYAEATEQPMQITGATAGPGGFGPQAAINFYGTAIDTVF
                     SGTRVYWLVAGDGAGARIVTLPASSGSNQPPASYPATVELRQHTTYFSALLTKDGQNF
                     FGALVSPAAADQILHVPHLDPKSTQLAQLEVALQGVVAFPHDVKVAVNGTSVGDVTFF
                     GQNKGSLKIALPPGVLRDWDNTVTLSSQNGDFDISLVESIRITYPHTYVADSDQLEFT
                     GRAGDELHVSGFSGAPMVLDVTDPNEPVRLTPAVSSTNGEYEVALQVPWTTTNPASPV
                     RHTLLALADDRIAAAAGVLANHPSHWHSPQAGADIVMVTAGAFEGMLGPLVRAHQAEG
                     KSSAVVPVAELYDEFNFGERSPFAIRQFLQSATTNWKKTPAYLLLNGRASFDPRNYLG
                     LGSEDLVPTRLLPVTGLMTASDDWFSDFTDSGKSTLATGRLPVGTADEEKTVVGKIAA
                     YESQSNSGVWTSQALMVADRDDTESFTQDTKTVQANLPATMQVTDIFTGTVGTAAAQQ
                     DIVNGINAGQLLVNYLGHGSEEQWSGSDIFNTGSVSSLSNGSQLPVFLIMNCLNGFFQ
                     DVYAQPLGVTLLLAPNGGGVAVLASTGLNQPAPQSQLDGFVLQNALSAARPTLGESIL
                     KAKSQITDVDVRRTYVLFGDPAMRVQPDAAAQAH"
BASE COUNT          792 a         1238 c         1208 g          800 t
ORIGIN      
        1 ctgccctcgc catcaccatc gctgctacga tctacctcgg cgtcctcccc aatcgggttc
       61 tccagtacgc ccgggaatcc gcccagacgc tgctcacggc gccgacccct gccggcactg
      121 tccaggctca ggccaaggcg gttggccagc cgtgatttct tgtggaaatc ctcaaaacgc
      181 acatttgaca agggccttga gccggtgcat catgtcacca cgagtggatg ctgcgaagtg
      241 actctggctg gaggagaaga aatgtccaca ttggttcgca tgctttggac agaggatgcg
      301 ggacaggaca tcgccgaata tgccgtgatg ttggcggtga ttctggttct ggtcgtcggg
      361 accgtccgac tgattgggtc gaatgccaac aatgcttttt cgcaggtcgc cagttcgatc
      421 cagtagccgg aacagcccag agcgctgctc gtcatattga aacagacaga agggtttcat
      481 gactcgattt accccaagct tcgcgtttac cgcctcctat tggtaccagg aatggcggct
      541 gcgacccggc ttggggtgag catgaactga aaatcacccg aagtaacaag cttcagtcgc
      601 aagccgcgat caaagatcgc ggcttttgtt ttttgggcaa accacacaag gagcatcgac
      661 ctcgatgatc gtcaacatgt cagaaaaagc taccgagcag gaaatcaacc acgtgatcga
      721 gcgaatccgg gaggctggat accagcctca cgtcacgcgc ggcaccgaac gcacgattgt
      781 ggctgccgtg ggcagcggac gtcgccatga gatcgaagct ctgcaggttg cccccggcgt
      841 ggacaacgtc gtcccaatcg cgcaaccctt caagctggtc agccgccagg tccggcccga
      901 acgctcggtt gtgaaggtcg acggagtggc gatcggcggt ccggaggtcg tcgtcatcgc
      961 cggaccctgc tcggttgaat cgcgcgagca gctgttcgat accgccctcg cggtcaagcg
     1021 cgcgggcgcg gccatgctcc ggggcggcgc ctacaagccg cgcacttccc cgtacgactt
     1081 tcagggtctc ggactcgaag cgctcaagat tctgcgcgag gcgaaagaac gaactggcct
     1141 cccggtggtc accgaagtga tgagcactga agatgtcgac gtcatctccg agtatgtcga
     1201 catgctgcag gtgggtgcgc ggaacatgca gaatttcgct ctcttgcgcc ggctcgccac
     1261 cgcaaaaaag ccgatcctgt tgaaacgcgg cccttctgcg acggtcaagg agtggctcct
     1321 ggccgccgaa tatcttctca ccggaggcaa ctctcaggtg gtgctgtgcg agcgcggaat
     1381 tcgcacattt gagactgaaa cccgcaacac catcgacctg gcggccgtag ctctggcgcg
     1441 tgacctttca cacctgcccg tgattgccga cccatcccac ggaaccggta agcagagcct
     1501 gattgcagcg gtttcacggg cagcggttgc ggtgggagcc gatggcctga tcatcgaggt
     1561 gcatccctgc ccggaacgcg cgctttccga tggcgctcaa tcgctggatt ttgcgggatt
     1621 cgccagagtg atgcaggcac tgaaagaacc ccttcggcga acgtcaggac tgcccgcggt
     1681 gtcgtagggc acgacgaagt agcgagagga tagtcagtgc gcctgcgcag ccgcatccgg
     1741 ctgcaccctc atcgccggat cgccgaagag aacatacgtc cggcgcacgt ctacgtcagt
     1801 aatttgcgac ttcgccttca gaatcgactc gcccagcgta ggccgtgctg cactcagggc
     1861 gttctgcagg acgaatccgt cgagctgcga ctgaggtgcc ggctgattca gcccggtcga
     1921 tgccaatacg gcaacgccgc ccccgttggg cgccagcaac agcgtcacgc ccagaggctg
     1981 ggcgtacaca tcctggaaga atccattcag gcaattcatg atcaggaaga cgggaagctg
     2041 cgatccgttg ctcagcgaac tcacggaccc cgtgttaaag atgtccgatc ccgaccattg
     2101 ctcttctgac ccatgcccca ggtagttcac aagcagctga cctgcgttga tcccgttcac
     2161 gatgtcctgc tgagcagcag ccgtccccac ggtcccggtg aaaatatcag tgacttgcat
     2221 ggtcgcaggg agattcgcct gaaccgtttt tgtgtcctgg gtgaagctct ccgtatcgtc
     2281 cctgtcggcc accatgagag cctgcgaagt ccacacaccg gagttggatt ggctttcgta
     2341 ggcggcgatc ttgcccacta cggtcttctc ttcgtcggct gtgccaactg gcaatcggcc
     2401 tgtagcaagc gtcgactttc cgctgtcggt gaaatcggag aaccagtcat cggaggccgt
     2461 catcaatccg gtcaccggga gaagccgtgt tgggaccaga tcctcggatc ctaagccgag
     2521 atagtttcgc ggatcgaacg atgcgcggcc gttcaacagc agatacgccg gggtcttctt
     2581 ccagttcgtc gtcgcagact gcaggaactg ccggatggca aacggactgc gctcgccgaa
     2641 gttgaactcg tcgtagagtt cggcgacggg cacgacggca gaggacttcc cttccgcctg
     2701 gtgagcacgg accaatggac cgagcatccc ctcaaatgcc cccgccgtga ccatcacgat
     2761 gtcggctccc gcctgcggac tatgccagtg cgacggatgg ttggccagca ctccggccgc
     2821 ggcagctatg cggtcgtcgg ccaacgccag cagggtatgc cgaacaggag acgcaggatt
     2881 ggtagtcgtc cagggcacct gcagcgcgac ttcatattcg ccattggtcg agctgactgc
     2941 tggcgtcaaa cgcaccggtt cgttcggatc ggtaacatcg agtaccattg gcgcgccgct
     3001 gaaaccgctt acgtgcaatt cgtcgcctgc gcgcccggtg aattccagtt ggtcagaatc
     3061 cgccacatac gtgtgcgggt aggtaatccg gatggattcg accagactga tgtcgaagtc
     3121 gccgttctgc gagctcagcg tgacggtatt gtcccagtct cgcaacactc ccggtggaag
     3181 agcgatcttc agacttcctt tgttctgacc gaagaacgta acgtcgccaa ccgaagttcc
     3241 gttcaccgca accttgacat cgtgcgggaa ggcaacgaca ccctgtagcg cgacttccag
     3301 ctgcgccagt tgggtcgatt tcgggtcgag atgcggcacg tgcaagatct gatcggcggc
     3361 tgccggggac accagggcgc caaagaagtt ttgcccgtcc ttcgtcagca atgccgaaaa
     3421 gtaggtggtg tgttgtctta gttcgacagt cgcgggatag ctggctgggg gctgattcga
     3481 ccccgaagag gccggcaacg tgacgatccg cgcccccgct ccgtctcccg ccaccagcca
     3541 gtagactcgc gtacccgaga acacggtgtc gatcgcggtg ccgtagaaat tgatcgcggc
     3601 ttgcggaccg aagccgccgg gccccgccgt cgcgcctgta atctgcatgg gttgttcggt
     3661 ggcctcggca tacaagtgta acttcgcagg atcgacgttg ggatcgagcc cggccttcag
     3721 cagcgcaggc tgcggcacgc gataccatcc ctcgcgcttc acccaaatct tcaccgctgg
     3781 ttttgccgcg agttcgaact gcttctgaag ctgactcggc gtcggctccg attgcagccg
     3841 tacggcctca agcgcgtggc tctccgcgct atcggtcgtt tcaaccttta cctgggcaag
     3901 ctggctgaac atgtgcgtct cggcggccga gacttcactg ccagtctgca tgctcgtttc
     3961 gagcgacacc ggtccgtgca gggtgcgcac gccgttgaca tcgatgtctt ccagccaata
     4021 ggaggcaccg gcgactgg
//
LOCUS       DASPUL010000071         5026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_558537, whole genome shotgun sequence.
ACCESSION   DASPUL010000071 DASPUL010000000
VERSION     DASPUL010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558537"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VEJ00_01720"
     CDS             <1..569
                     /locus_tag="VEJ00_01720"
                     /inference="COORDINATES: protein motif:HMM:NF016138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HXW99902.1"
                     /translation="YILDGQTFDLDGVRSIANNPRHVATSPFTSYNYHGKGNFASMVD
                     AVVLGATEVDTNFNANVVTHSDGRLLHGIGGWQNCLAAGCTILAVPSFRDRIPVIVDE
                     VTTLCGPGELIDVVATERGIAINPRRQDLIDAVKGSKLPIRPLEDIKKEVERICGGKP
                     QRPKHGERPVAIVKWVDGTVLDTVWQTG"
     gene            complement(588..2747)
                     /locus_tag="VEJ00_01725"
     CDS             complement(588..2747)
                     /locus_tag="VEJ00_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HXW99903.1"
                     /translation="MNRPLAISCPSLLAIVVFALCFGISAQTVSVDFTPSHVVNKFSP
                     IRALGSTVDRVPSNATDVFFRPDQIQKVLSAGWGVMSYRQNTDLFVQAWHWNPKGTWS
                     DPSGKGYFTGDATPTSEMIRHSYGYSLPHRGFTRNGGSEFDGYSRLDDGDPNSYWKSN
                     PYLTKEFTGEDDARHPQWVVISLDKPESVNAIRIHWGDPYARDFEVQYWMGDGDAMDE
                     QDKGAWKTFPSGSKTDAKGGITTYGFGGAVTTKFVRVLMTRSSNTCDTHGSSDRRNCV
                     GFAIRELYLGTYDKSGFTDLLHHSPDQKQTLTLCSSVDPWHQPSDLYIAPDRMESGDQ
                     PGFDLFFTSGITRGLPAIIPVALLYSTPEAAAAQMSYIKKRGYPVSYIEMGEEADGQY
                     MLPEDYGALYLQFAAALHRVDADFKLGGPAFQGVTEDVKAWPDDQGRTSWFGRFLDYL
                     KARGRLQDLAFMSFEHYPYDGCETPWTQLYREPELITNIMKVWREDGLPPGVPLLDTE
                     TNAHGGEASVDIFGALWLADSFGGFLGAGGQSTHYYHDLSYSPPHPACANSWGTYHLF
                     MVDDHYQITQPTSQFFAAQLITQQWAEPKDAEHQQYLAASDVKDSAGHVLVTAYALHR
                     PDDQWSVMLINKDHDHAHEVRIAFHDADGKRDSAFSGQVTVITFGKEQYQWHPARKKG
                     YAEPDGPPLTSQVNGEESTRYTLPAASVTVIRGRLSQ"
     gene            2941..3726
                     /locus_tag="VEJ00_01730"
     CDS             2941..3726
                     /locus_tag="VEJ00_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HXW99904.1"
                     /translation="MVLRVFPDKSSLGRAAAEQAANAIRRAIAVRGHARIIAATAASQ
                     LEFLDALTKTPGIDWSKVEAFHLDEYVGLPITHPGSFRKMLLAQLVNKTGITNYHLLD
                     GDAPDPDAVVREASKALAVAPIDIAFLGIGENGHIAFNDPPADFNTEEPYIIVELDQA
                     CRRQQVGEAWFSDISQVPKRALSMSARQILKAKEILAVVPDKRKAEAIKACFEGEISP
                     MAPASILRRHPNATVYLDVNSASLLGPRLLTPLMEKSQVTVSP"
     gene            complement(3716..>5026)
                     /locus_tag="VEJ00_01735"
     CDS             complement(3716..>5026)
                     /locus_tag="VEJ00_01735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXW99905.1"
                     /translation="VGHTTRVRWLLVFCLFVLSAVSYLDRVNISIAGGSIADAYHLND
                     VQLGKVFSAMLVGYALFQTVGGRLADRFGPRRVLTLGVVWWGIFTALTALVPARLEGA
                     LLLFMAVRFLLGAGEAVIYPSANQFIARWIPVSERGLANGWIFAGVGAGAGLTPPFIT
                     YLMFHYGWRSSFWACAVVGLIAGGLWYIAARDTPAEHPDVSASELVTIQSGLSIAKSG
                     SDPFDPKPDKAPIPWTQVVQNREVLAVTLSYFCYGYVGWLFFSWFYRYLAKVRGLDLK
                     ASAFYSMLPFLAMLVGCLVGGIVNDRLTKWHGPRMGRCGVAAFALVLAAVFIACGAQV
                     HSARLASVVLAGGAGALYLSQSSFWSVTADIAGQSSGFVSGFMNTGNQIGAAITATLT
                     PWIAAQFNWTASFAVAAVLCLLGAVCWLIVDPTRMMVPSNAATD"
BASE COUNT         1083 a         1482 c         1537 g          924 t
ORIGIN      
        1 actacatcct cgacgggcag acgttcgacc ttgacggcgt tcgatccatc gccaacaacc
       61 cgcgccatgt cgcgacctcg cccttcactt cctacaacta ccacggcaag ggaaattttg
      121 catcgatggt cgacgcggtg gtgctcgggg cgaccgaagt cgacacaaac ttcaatgcca
      181 atgtcgtcac tcattcggac gggcggcttc ttcacggcat cggcggatgg cagaactgtc
      241 tggcggcggg atgcacgatc ctggctgtgc cgtcgttccg tgacaggatt ccggtgattg
      301 ttgacgaagt gacgacgctg tgcggtccgg gggagctgat tgatgtggtg gccaccgaac
      361 gcgggatcgc gatcaatcca cggcggcagg atctgatcga cgcggtcaag ggctcaaagc
      421 tgcccatccg tccgctcgag gacatcaaga aggaagtcga gcgcatctgt ggcggcaagc
      481 cgcagcggcc gaagcacggc gagcggccgg tggccatcgt aaagtgggtc gacggcacgg
      541 tgctcgatac ggtgtggcag accggctaag gtcattcctt cacattgtca ctgtgacaag
      601 cggccgcgaa tcactgtcac agaagccgct ggcagcgtgt agcgtgtact ctcttctccg
      661 ttaacctggg acgtcaacgg gggcccgtcc ggctccgcat atcccttctt gcgcgcagga
      721 tgccactgat actgctcctt gccaaacgtg atcaccgtga cctgcccgct gaaagccgag
      781 tctcgcttgc cgtccgcatc atgaaaggcg atgcggacct cgtgtgcatg gtcgtgatcc
      841 ttgttgatca gcatcacgga ccactgatcg tcggggcggt gcagagcgta ggccgtgacc
      901 agcacgtgcc ctgcagagtc cttcacgtcg ctcgcggcaa ggtactgctg atgctcagcg
      961 tccttgggtt cagcccactg ctgggtaatc agctgcgcag cgaagaattg cgacgtcggc
     1021 tgcgtgatct gatagtgatc gtccaccatg aaaaggtgat acgtgcccca actgttggcg
     1081 catgccgggt gcggcgggga atacgacaaa tcgtgatagt agtgcgtgga ttggccgccg
     1141 gcgccgagaa agcccccgaa ggaatcggcc agccacaggg cgccaaaaat gtcgacagaa
     1201 gcctcgccgc cgtgggcatt ggtttcggta tcgagcagcg gaacaccggg cggcaagcca
     1261 tcctcgcgcc agactttcat gatgttcgtg atcaactccg gctcgcgata gagctgggtc
     1321 cacggcgttt cgcatccatc gtacggataa tgctcgaacg acatgaacgc caagtcctgc
     1381 aggcgcccgc gggctttcag gtagtcgagg aaacggccga accaagacgt gcgcccctga
     1441 tcgtcgggcc aggctttgac gtcttccgtg acgccctgaa aggctggccc gcccagcttg
     1501 aaatccgcgt ccacgcggtg cagtgcagcc gcgaactgca gatagagcgc gccatagtct
     1561 tcgggcagca tgtactgacc gtcggcctct tcgcccatct cgatgtaaga gaccggataa
     1621 ccacgcttct tgatgtagga catctgcgca gcggcggctt ccggcgtgct gtaaagcaag
     1681 gcgactggga tgatggcggg caggccgcgc gtgattccgc tggtgaagaa cagatcgaag
     1741 ccgggctgat ctccggactc catgcgatcg ggcgcgatgt agagatcgga gggctggtgc
     1801 cagggatcga ccgaggagca caacgtcaag gtctgcttct ggtccggaga atggtgaagc
     1861 agatcagtga acccgctctt gtcataggtc cccagataaa gctcgcggat agcgaatccg
     1921 acgcagttgc ggcgatcgga ggaaccgtga gtgtcgcagg tgttggagga gcgggtcatg
     1981 agcacgcgga cgaactttgt agtcaccgcg ccgccgaagc cgtaggtggt gatgccgcct
     2041 tttgcgtcgg tcttgcttcc tgaggggaaa gtcttccacg cgcctttgtc ctgctcgtcc
     2101 atggcgtcgc cgtcgcccat ccagtactga acctcgaagt cgcgagcata cggatcgccc
     2161 cagtggatgc ggattgcatt cacgctctcg ggcttgtcca gcgagatgac gacccactgc
     2221 gggtgccgcg cgtcgtcctc tccggtgaac tctttggtca gatagggatt gctcttccag
     2281 taggaattcg gatcgccgtc gtcgagtcgc gagtaaccgt cgaattcgct gcccccattc
     2341 cgggtaaagc cgcgatgggg gagcgagtat ccgtaagaat ggcggatcat ttcgctggtc
     2401 ggagtcgcat cgccggtgaa atatcccttg ccggaggggt cactccatgt gcccttcgga
     2461 ttccagtgcc aggcctgcac gaaaaggtcg gtgttctgac gatagctcat gactccccag
     2521 cccgccgaaa gaaccttctg gatctgatcg ggacggaaga atacgtcggt ggcgttgctg
     2581 gggacgcggt caacggtcga acccagggcc cgaattggac tgaacttgtt cacgacgtgg
     2641 ctaggcgtga agtcgacgga gaccgtctgg gcgctgatac cgaaacacaa ggcgaacaca
     2701 acgatggcca aaagagatgg acatgagatc gcaagagggc ggttcatagg agtgtcctga
     2761 tctgcagatg ttccgcgtag aagtgataca acaacctgcg cgcttctttc cacgcggcgg
     2821 tacggcagtt tttcatggaa tgaaattccg gtcccctgcg agcctaagcg cctgacacac
     2881 gtctgtataa ttcttcgtct taccaaggtt cgattcctgc agtcgctcga agggcgctct
     2941 atggttctgc gagttttccc cgacaaatcc tcgttaggcc gcgccgccgc ggagcaggcc
     3001 gccaacgcaa ttcggcgcgc gattgcagta cgcggccacg ctcgcatcat cgccgcgacg
     3061 gcagcatcgc agctcgagtt tcttgacgcg ctcaccaaga ccccgggaat tgattggtcg
     3121 aaagtggaag cgtttcacct tgacgagtac gtcgggcttc cgatcacgca tcccgggagt
     3181 ttccgcaaga tgctgctcgc gcaactggtg aacaagaccg ggatcacgaa ttaccacctg
     3241 ctcgacggcg acgcgcccga cccagatgct gtcgtgcgcg aggcatcgaa ggcactcgca
     3301 gtggcgccga ttgacatcgc cttcctcggt atcggggaga acggacacat tgcgttcaac
     3361 gatccgccgg cagacttcaa tacggaagag ccctacatca tcgtcgaact tgaccaggcg
     3421 tgccggcggc aacaggtcgg ggaagcgtgg ttctccgaca tctcgcaggt tcccaagcga
     3481 gctctctcca tgtcggcgcg gcagattctc aaagccaagg agatactggc tgtggtcccc
     3541 gacaaacgca aggcagaggc gatcaaggcg tgtttcgagg gagaaatcag cccgatggcg
     3601 ccggcgtcga ttctgcgacg gcatccgaac gcgaccgttt atctcgacgt caattccgct
     3661 tccctgcttg gtccgcggct gctgactccg cttatggaaa aatcgcaggt tacggtcagt
     3721 ccgtagccgc gttcgacgga accatcatcc gagtcggatc gacgatcaac cagcacaccg
     3781 cgcccaacag gcacaagacc gccgctacag cgaaagatgc ggtccagttg aattgcgcgg
     3841 caatccacgg cgtgagcgtc gcggttattg cggcaccaat ctgattgccg gtattcatga
     3901 atccggaaac gaaaccggat gattgccccg caatgtcagc agtaaccgac caaaacgagc
     3961 tttgcgacag atagagagcg ccggcgcccc cggcgagcac aacgctggcc agacgggcgc
     4021 tgtgcacttg cgcgccgcag gcaatgaaga cggcagccaa cacgagcgcg aacgccgcca
     4081 cgccgcaacg tcccattcgc ggaccatgcc atttcgtcag gcggtcgttg acgatcccgc
     4141 cgacaagaca gcccaccagc atggccagaa acggcagcat ggagtagaac gcgctggcct
     4201 tgagatccag cccgcgcact ttggccagat agcgataaaa ccagctgaag aagagccatc
     4261 cgacatagcc gtagcagaaa taactcagcg tgactgccag gacttccctg ttctgcacga
     4321 cctgcgtcca aggtatggga gctttgtctg gtttgggatc aaacggatcg cttcctgatt
     4381 ttgcgatgct aagtccggac tgaatcgtca caagctcgga tgcggaaaca tccggatgct
     4441 ctgcgggagt gtcgcgggcg gctatgtacc agagccctcc tgcaataaga cctacgacag
     4501 cgcacgccca aaacgacgac cgccaaccgt agtggaacat caggtacgtg atgaaaggag
     4561 gggtgaggcc agcgcccgcg ccaaccccgg cgaaaatcca gccgttggcc aagccacgct
     4621 cgcttaccgg aatccagcgc gcgatgaact gattggccga ggggtagatg actgcctcac
     4681 cggctccaag cagaaagcgc acagccatga agaggagtag ggcaccttcg aggcgcgcgg
     4741 gaacgagcgc ggtgagtgca gtgaagattc cccaccagac aacccccaag gtgagcacgc
     4801 gacgtggacc gaagcggtcg gccaaacggc cgccgacggt ttgaaacaag gcatagccga
     4861 cgagcatcgc actgaaaacc ttgcccagct gcacatcatt gaggtggtaa gcgtcggcga
     4921 tcgatccgcc ggcaatggaa atgtttacgc gatcgaggta agacacggcg ctgaggacaa
     4981 aaaggcagaa aaccagcagc catcgcacgc gagttgtatg accaac
//
LOCUS       DASPUL010000072         4180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1791911, whole genome shotgun sequence.
ACCESSION   DASPUL010000072 DASPUL010000000
VERSION     DASPUL010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791911"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VEJ00_01740"
     CDS             complement(<1..690)
                     /locus_tag="VEJ00_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HXW99906.1"
                     /translation="MRILIAEDDEALARFVRQGLESENYSVDIFADGEQARAAAIDSE
                     YDVLILDLNLPKLDGVHVLRQVRLKKPNLPVLILTQRTRVEDRVQCLDTGADDYVPKQ
                     LSGTSLDILHIQPDLLAKIRAAAAWRRSHRINPSARKPPRSTLIESVDTGSATPAIVV
                     IGTSTGGPKALQETLPLLPRELPVPVLVVQHMPAGFTSAFAQRLNALCSVTVREAVDR
                     DVLQPGVVYIAP"
     gene            884..2221
                     /locus_tag="VEJ00_01745"
     CDS             884..2221
                     /locus_tag="VEJ00_01745"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXW99907.1"
                     /translation="MKEISGWSLGLQLWSLTTLKSIVVPGGILLLGVVLLAYSGWLTL
                     PLPALTFLYYCALSGGLLLAWRFHSSRVFLALLVLFLAQQAIELSGLGHGGAGASAST
                     AFGAVAVLVPLNFVLIALMTERGFTLSATAPVGLLLFVECAIVAVVARGAQGAPVPGH
                     SHHLAAAISLPGYAQIAGAGAGAILLLRSIFTRKPAESALFWSLASYFLALHFLGSAR
                     LATLYSATAACTLAASIIENSYLLAYHDELTALPSRRAFNDALARLQDPYSIAVVDID
                     HFKRFNDTYGHDTGDQVLRLVAANLSRVTGGGHAYRCGGEEFAILFPGKTTVEVSEHL
                     ERLRAQIESSEFHMRRGERRQVPRGPDRRTRPARSRRTGHAIRRLAHGQSPAPLSVTV
                     SIGVASSGKNRLHPEHVLQAADKALYRAKANGRNRIETASDRRRAGLKTAGIA"
     gene            complement(2681..>4180)
                     /locus_tag="VEJ00_01750"
     CDS             complement(2681..>4180)
                     /locus_tag="VEJ00_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HXW99908.1"
                     /translation="IATHFVQVRLSGDLAFFQGLCKELLEEEARRPGEVIHHDFVQQK
                     TTGVEEYRAALAGVSWDDIVEQSGIARAQIRQIAEVVMRSKNMIACWAMGLTQQRQAV
                     ATIQEIVNLTLLGGHIGRPGNGVCPVRGHSNVQGDRTMGIWERMPDSFLDKLRDVFGF
                     EPSRKHGWDVVDSIKAMHRGDAKIFFAMGGNFLSATPDTEFTAEALSRCDLTVHVSTK
                     LNRAHVISGRQVLILPCLGRTDRDRQTSGGQFVTVENSMGIVHMSRGSLDPPSDQLLS
                     EPAIVARLAVATLGDHARVQWMHLVEDYDRIRDLIAQIVPGCEDMNRRVRQRGGFYLP
                     NAARNNDFKTRSGKAHFTVSVMEPIPLGPGQFLLTTLRTHDQYNTTIYGLDDRYRGVF
                     HGRRVVLINPEDVREHGWKAGDRLDITSHFWNEGREELRTAERFVVVPYEIPRRCVAT
                     YFPEANVLVPIGSVALRSNTPTSKAVVVSFCLSNANDAAACVDESHVVG"
BASE COUNT          803 a         1272 c         1257 g          848 t
ORIGIN      
        1 tggtgcgatg tatacgacgc caggctgaag cacgtcgcgg tcaacggctt cgcgcaccgt
       61 tacggaacac aaggcgttga gacgttgcgc gaaggccgac gtgaatcccg ccggcatatg
      121 ttgcacgacg aggactggca caggaagctc gcgcggaaga aggggcaggg tctcctgcag
      181 agctttgggg ccgccggtcg aagttccgat gacaacaatg gccggggtgg cggaaccggt
      241 gtcaaccgat tcgatcaggg ttgatcgagg tggttttcga gccgatggat tgatcctgtg
      301 agaacggcgc caggctgcgg cggctcgaat cttggccagc aggtccggct ggatgtgaag
      361 aatgtcgagc gatgtgccgg agagctgctt gggaacatag tcgtcggcgc ccgtgtccag
      421 gcactgcaca cggtcttcga cccgggtgcg ctgggtcagg attagtaccg gcagattcgg
      481 ttttttcagc cgcacctggc gcaggacatg gactccgtcc agcttgggga ggttcagatc
      541 gagaatgaga acatcgtatt cggagtcgat cgcggccgct cgagcctgct cgccgtcggc
      601 aaagatgtcg acgctgtaat tctcgctctc gagcccttgg cgaacaaacc tggctaaagc
      661 ttcatcatct tccgcaataa gaattcgcat aacgtaaacc gtccttaatc caaagccagt
      721 ctagttagaa acggctcaag tgcagccaga acagcgctcg cacgaaggta tctaagtgca
      781 gaagtggagc tcgatttcga atcgggagag gagcgttcag ggcgccgcct gacgccgaac
      841 cttggtggcc aattcctcgc aacctgctcg aagattgata attttgaagg aaatttccgg
      901 ctggagtctt gggttgcagc tttggagtct caccaccctg aagtcaatcg tggttcccgg
      961 cggaatcctg ctgctcggag tcgtcctgct cgcatattca ggatggctca ctcttcccct
     1021 gcccgctctg accttccttt actattgcgc tctttccggg ggacttctgc tggcgtggag
     1081 atttcactcc agccgcgtct ttcttgccct gctggttctt tttctcgccc aacaggcgat
     1141 tgagctgtcc ggattggggc acggcggagc cggagcttcc gcgtcgacgg ccttcggtgc
     1201 ggtggccgta ctcgttccgc tgaattttgt gctgattgcg ctcatgaccg agcgtggctt
     1261 cacgctttcc gcgaccgcgc ccgtcgggct gctgctgttc gtcgagtgtg cgatcgtggc
     1321 ggtggtcgcc cgcggcgccc agggcgcgcc ggtgcccgga cattcgcatc acttggcagc
     1381 agcgatttct ttgcccggct atgcacagat cgccggagcc ggggcaggcg caatcctctt
     1441 gcttcgatcc atctttacgc gcaagcccgc cgagagcgca cttttttggt cgctggcctc
     1501 ttacttcctg gcgctgcatt ttctgggatc ggcgcgcctc gcaaccttgt actcggccac
     1561 cgcagcctgc accctggcgg cttcgattat cgagaattct tacctgctgg cttatcacga
     1621 cgaactgacg gcgctgccct cccggcgcgc cttcaacgat gccctggcac ggctgcagga
     1681 tccgtattcc atcgccgttg tcgacatcga ccacttcaag cgattcaacg acacctatgg
     1741 tcatgacacc ggagaccagg tgctgcgcct ggtggcggcc aacctttcgc gagtcactgg
     1801 cggcggacac gcgtaccgct gcggcggaga ggagttcgca atcctgtttc ctggcaagac
     1861 gaccgtcgaa gtttcggaac accttgagcg attgcgcgcg cagatcgagt cctcggaatt
     1921 ccacatgcgc cgcggggaaa ggcggcaagt tccacgcgga ccggaccggc gaaccaggcc
     1981 agctcgcagc cgccggactg ggcacgcgat acggcggctc gcccacggac aatctccggc
     2041 ccctctctcc gtcactgtga gcatcggcgt cgcgagctca ggaaagaaca ggcttcatcc
     2101 cgaacacgtg ttgcaggcag cagacaaggc gctgtatcgc gccaaggcca acggaagaaa
     2161 tcgaattgaa accgcgtctg accggcgccg cgccggcttg aagactgcgg gaattgcctg
     2221 attggcacga ctggcgtgaa tcgaggcgaa aatcggctca ggtggaacgt ctggcccgga
     2281 ccgtcaactg caggacggga agtgtgaggg cccgggcccg tccgttttcg caaacaccgc
     2341 aggccgtgcg gtcagcccgc agtcgagggt gaggttgggc gtttccggcc agtgccttgg
     2401 cacaccgggc atttggagcg gataaacgcg acaccgtctg acttcccgtt caagtcaacc
     2461 ggctttgcga catatccggt accgccgcag ctcgggcagt cgtccgggct cacccctctt
     2521 ccccctcatt aagtaagcgt attctccaac acttctgcct cgccttcgca gaagacgcat
     2581 ttgcgttacg aatcacgaac aaaagccggg aggcaaacag agcgaaccct atcagcgctt
     2641 gcggaggttt ggcgggaaga ctggaacacc gccggattcg tcagccaacg acgtggctct
     2701 cgtctacgca cgcagccgca tcattggcgt ttgacaagca gaaactcacg actacagctt
     2761 tcgacgtggg ggtattgctg cgcagcgcca cgctgccaat cggcaccagt acattagcct
     2821 cgggaaaata agtggcgacg cagcgccggg gaatctcgta aggtactacg acaaaccgtt
     2881 cggccgtgcg caactcttca cggccttcgt tccaaaagtg gctggtaata tccagacggt
     2941 ctcccgcctt ccacccgtgt tccctcacat cctccgggtt gatcaggacg accctgcggc
     3001 catgaaagac gcctcgatag cgatcgtcga gaccatagat agttgtgttg tactggtcgt
     3061 gcgtacggag ggttgtcagc aagaactgcc ccggcccgag gggaatgggc tccatcaccg
     3121 ataccgtaaa gtgggccttc cctgaccggg tcttgaaatc attgttgcga gccgcattgg
     3181 gcagatagaa tccgcctcgt tgccgcacgc gccggttcat atcctcacaa ccgggcacga
     3241 tctgcgcgat caggtcgcgg atgcggtcat agtcttcgac caggtgcatc cactgaaccc
     3301 tcgcgtggtc gccgagcgtt gcaaccgcca ggcgagccac aatcgcgggc tcgctgagca
     3361 attgatccga aggcggatcc aagctgcccc gcgacatgtg tacgatgccc attgaattct
     3421 cgacggtgac gaactgccca cccgacgttt gccgatcccg atcggtgcgc ccaagacacg
     3481 gcaggatcag cacttgtctt cccgaaatga catgggcccg gttcagcttg gtcgaaacgt
     3541 gcaccgtcag gtcgcaccgt gacaaggcct cagcggtgaa ttcagtgtca ggtgtggcgg
     3601 agagaaaatt tccgcccatg gcgaagaaaa tcttcgcgtc gccacgatgc atcgctttga
     3661 tcgaatcgac aacgtcccag ccgtgcttgc gcgaaggctc gaagccgaag acatcgcgca
     3721 gcttgtccag gaaactgtcg ggcatccgct cccagatgcc catggtgcgg tcgccttgga
     3781 cattggagtg gccacgcacc gggcagaccc cgttgcccgg tcgcccgatg tgaccgccca
     3841 gcagcgtaag gttgacgatc tcctggatgg tggcgacggc ctgccgttgc tgcgtcagcc
     3901 ccatagccca gcaggcgatc atgttcttcg agcgcatcac gacctcggca atttgccgga
     3961 tctgtgcgcg cgcgatgccg ctctgttcga cgatgtcgtc ccacgaaact ccggcgagcg
     4021 cggcgcgata ttcctcaacg cctgtggttt tttgctggac gaagtcgtga tggatcactt
     4081 cccctggccg ccgcgcttct tcttccagaa gttccttgca gaggccttgg aagaacgcca
     4141 ggtcaccgct gaggcgaact tgaacgaaat gggtcgcgat
//
LOCUS       DASPUL010000073         4662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1107166, whole genome shotgun sequence.
ACCESSION   DASPUL010000073 DASPUL010000000
VERSION     DASPUL010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107166"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VEJ00_01755"
     CDS             complement(<1..268)
                     /locus_tag="VEJ00_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HXW99909.1"
                     /translation="MHELSIAMSIVDTALEESKRRGVQVSAVHLRLGALSGVVKEALF
                     FSYEMACQDTPLQGSRLIVEDVPVVVFCPQCNEPRTLPSVQSFAC"
     gene            complement(388..666)
                     /locus_tag="VEJ00_01760"
     CDS             complement(388..666)
                     /locus_tag="VEJ00_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003215863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXW99910.1"
                     /translation="MNKADLVDRIAGACEISKAQATTAIDTAVESVTSALRKGDRVAL
                     IGFGTFSVSQRKARNGRNPQTGATIKIAARRVAKFTPGAELKKAVNKK"
     gene            1064..1801
                     /locus_tag="VEJ00_01765"
     CDS             1064..1801
                     /locus_tag="VEJ00_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXW99911.1"
                     /translation="MKSASDMGRSPAERRVTRCDCQLRARAQSLLAAARIPRRYEHCE
                     LANYDTDFPGAHPSLAEAHFIASNFAKKCDPRGDKGLLIIGKIGTGKTHLAVGILKEL
                     ILTRGIPCLFYDYRELLKEIQNSYNTTVQTTELDVLRPVFETDVLVLDELGAVKPTEW
                     VWDTVSLILNTRYNDNRTTIITTNFEDHPAAGAAGSISPARAAARGETLGDRIGERMR
                     SRLHEMCRIIPLDGADFRQKFRSASFR"
     gene            1988..3145
                     /locus_tag="VEJ00_01770"
     CDS             1988..3145
                     /locus_tag="VEJ00_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99912.1"
                     /translation="MLSTSLEFAPERDAEVFAAVPAAPAVFMLRGDDPQAEPYISKTA
                     NLRRRLQRLLGPVEERTKKLNLRHRISSIEYTPTGSDFESGFLLYTALRTAFPKTYSR
                     RLRFRFTPFVKLHLENEYARASVTTRLGRLNGPALYYGPFVSRVAAEKFMNDSLDFFK
                     MRRCVDDLHPDPKFPGCIYSEMKMCLAPCFKGCTDDEYAAEVGRVGAYFDSSGDSLVR
                     EIAAEREAASARLAFEEAAAIHVRAEKLKPIVSQLPEIVQRLDRLSALMVQPSHITDS
                     VAFFRIESGRILGPIQFCIRPTEHTKSRSMEARVQETLQLFPAAKSKSVLETMEHVAL
                     LKRWYYRGTRLGEIFFADAKGNLPLRRVVLGISRALRGEKEEQVAAPPGNC"
     gene            complement(3313..3834)
                     /locus_tag="VEJ00_01775"
     CDS             complement(3313..3834)
                     /locus_tag="VEJ00_01775"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXW99913.1"
                     /translation="MRKLLLLLVCLISPAIAQTVPEGIGQGYDGELKHVTSQLLALAE
                     ATPADKFAWRPSPGVRSTSEVYMHIAIANFYFLSVVGKQTMPTEIKPGFEKSVTAKAD
                     VISWLKRSLDAARDARAGETPKELERKVEFLKRSTTADAVYLRLIIHCNEHMGQLVAY
                     ARVTGVTPPWSKD"
     gene            4133..>4662
                     /locus_tag="VEJ00_01780"
     CDS             4133..>4662
                     /locus_tag="VEJ00_01780"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXW99914.1"
                     /translation="MIGQTVSHYRIVEMLGGGGMGVVYKAEDTLLGRFVALKFLPDDS
                     SQDPQALERFRREARAASALNHPNICTIHEIGQHDGHLFIVMECLSGQTLKHLINGRP
                     MEMERLLTLSTEVADALDAAHSKGIVHRDIKPANIFVTSQGYVKLLDFGLAKLTGDTA
                     KAAAAAASTVPNADVIV"
BASE COUNT          954 a         1532 c         1242 g          934 t
ORIGIN      
        1 gacaggcgaa cgactgcacc gaaggcagcg tgcgcggctc attacactgc gggcagaaga
       61 ccaccactgg cacatcctcc acaatcaggc gtgagccctg caaaggcgta tcttggcagg
      121 ccatctcata ggaaaagaac agcgcttcct tcactacccc ggagagtgcc cccagcctga
      181 ggtgcacggc gctgacctgc actccccgac gctttgactc ctcgagcgcc gtatccacga
      241 tgctcattgc aatggacagt tcgtgcatca gggtaccgag acaattctaa actgactccc
      301 gacgcttctg tcctgatctc cgctggcaaa agaaaaacgg cagggccgac cccctgccgc
      361 ttcttggaat ctcgaatctt gtacgcccta cttcttattc acagccttct tgagctcggc
      421 cccaggggta aacttcgcga ctcggcgggc agcgatcttg atagtcgcgc cggtttgcgg
      481 gttgcgcccg ttccgcgctt ttctctgcga cactgaaaaa gtgccgaagc ctatcagagc
      541 tacccggtct cctttgcgga gcgcggacgt aacgctttcg acggccgtat ctatcgcagt
      601 cgtggcctgc gctttgctga tctcacacgc cccagcgatc ctgtcaacca gatcggcttt
      661 gttcatagtc tctcctcgca ggtctcgttg ctgcccagcc tacaccggga cgacaaaatg
      721 gctatggctg agttgggaaa cctgaccgtt tcgtattgtg ctccctcttt ggcgaatgtc
      781 aactgtgaaa atccgcgccg gaagccggtt ttgcacctcc ataacccgaa ttggggctag
      841 cacggcggtt tttgctctca aggtgcgagt tttcctacac gttccacagc ccgactcccc
      901 gcttcacagg ttgttaacag gccctccccc attcccgctt gcgcgaaact gcctcccggg
      961 tgcaaagtcg aaaggtaaca atcccataca gctggaaggg cccgaagtga tcaaagctcc
     1021 cgcggatgtc tgcccgttgt gcgagggaac aggatggaaa accgtgaaat cggcgtccga
     1081 tatgggtcgt agcccagccg agcgccgggt gacccggtgt gattgtcagt tacgtgcccg
     1141 tgcccagtcc ctgctggctg ccgcgcgtat cccgcgccgc tatgagcact gcgagctcgc
     1201 caactacgac accgacttcc ccggcgccca cccctccttg gctgaggccc actttatcgc
     1261 ctcgaacttc gccaaaaaat gcgacccgcg cggcgacaag gggctgctga taatcggcaa
     1321 gatcggaacg ggcaagaccc acctggccgt cggtatcctt aaggaactga tcctcacccg
     1381 cggcattcca tgtctgtttt acgactatcg cgaactactg aaggagatcc agaactccta
     1441 caatacgacc gtccagacca ccgaactgga cgtcctccgc ccggtcttcg aaaccgacgt
     1501 ccttgtcctc gacgaactgg gcgccgtcaa gccaaccgag tgggtctggg acacggtcag
     1561 cctgatcctg aacacccgct ataacgacaa ccgcacaacg atcatcacca cgaatttcga
     1621 agaccatccg gctgccggag cggccggttc aatttcgccc gcgagggccg cagcccgcgg
     1681 cgagaccttg ggcgaccgca tcggcgagcg catgcgctcc cgcctgcacg agatgtgccg
     1741 catcatcccc ctcgacggcg ccgatttccg ccagaagttc cgcagcgcca gcttccggta
     1801 agccgcgcca cggatttgcg cggattcacg cggaccaaga attacctatc attctgagct
     1861 aaagcgcaga acctctgcac ctggctgccc tctctacaac tctctggggc aaaagcgcga
     1921 ttagctgaca gagactcttc tccgtagtct ctgtgcctcc gtggtgaaaa cgtgcttaga
     1981 atgaactgtg ctcagcacaa gcctagaatt cgcgcctgaa cgcgatgccg aagtctttgc
     2041 tgccgttccc gcggctccgg ccgttttcat gctgcgtggc gatgaccccc aagccgagcc
     2101 ttacatcagc aagaccgcca atcttcgccg acgcctgcag cgcctgctag gcccggtcga
     2161 agagcgaacg aaaaagctca atttgcgcca tcgcatcagc tccatcgaat acaccccgac
     2221 cggctccgac ttcgaatccg gattcctcct ctacaccgcg ctgcgcacgg cattccccaa
     2281 aacctactct cggcgcctgc gcttccgctt cacccccttt gtcaagctcc acctcgaaaa
     2341 cgaatacgct cgcgcctccg taaccacccg tctcggccgc ctgaacggcc ccgcgctcta
     2401 ctacggcccg ttcgtttcgc gagtcgcggc cgagaaattc atgaatgact ccctcgactt
     2461 tttcaaaatg cgccgctgcg ttgacgacct gcatcccgac cccaaattcc ccggctgcat
     2521 ctattccgag atgaaaatgt gccttgcccc ctgcttcaag ggatgcaccg acgatgaata
     2581 cgcagcggaa gtgggccgag tgggggccta tttcgattct tccggagact ctctggttcg
     2641 cgagattgcg gccgagcgcg aggctgcgtc agcccggctg gcctttgaag aggcagcggc
     2701 aattcatgtc cgcgcggaaa agctgaagcc catcgtcagc cagctacccg aaatcgtgca
     2761 gcgcctcgac cgcctctcgg cgctcatggt ccagcccagc cacatcaccg attcagttgc
     2821 gttcttccgc atcgaatctg gacgaatcct gggtcccatc cagttctgca tccgtccaac
     2881 ggagcacacc aagtcgcgat cgatggaagc ccgggtgcaa gaaaccctgc agttgttccc
     2941 ggcggcaaaa tcaaaatccg tgctggagac tatggagcac gtagcgctgc tgaagcgctg
     3001 gtactatcgc ggtacccgtc ttggcgaaat cttcttcgcc gacgccaaag gcaatctgcc
     3061 cttgcgccgt gtcgtcctgg gcatctcgcg tgccctgcgc ggcgaaaagg aggaacaagt
     3121 agccgcgcct cccggaaact gctagctgcg atcgattcgg gtgcaagatc acccttgcac
     3181 ggctgcctgt gtagactctg ctgtgaacca ccgccgccca acccagcgcg tgactcgcca
     3241 tgcagcaaga actccggcga gaatggttcc tacccacgtg acgaggcgcc gagattcttg
     3301 gccagggcgg ccctagtctt tcgaccacgg cggggttacg cctgttacgc gcgcataagc
     3361 gacaagctgc cccatgtgct cgttgcagtg aatgatcagg cggagataga cggcgtcggc
     3421 tgtagtgctt cgcttcagaa actcaacctt tcgctccagt tctttgggcg tttccccggc
     3481 ccgcgcgtcg cgcgcggcat ctaacgaacg cttcagccag gagatcacgt cggccttagc
     3541 ggttactgat ttctcaaatc ccggtttgat ctcagttggc attgtctgtt ttccgacaac
     3601 gctcagaaag tagaaattgg cgatggcgat gtgcatgtac acctcactcg tagagcgcac
     3661 accgggtgat ggccgccacg cgaacttatc tgcgggcgtg gcctccgcca aggccagcaa
     3721 ttgactggtt acatgcttta actcgccatc gtagccctgt ccaattccct ctggaacggt
     3781 ctgggcaatt gctggtgaaa tcagacaaac cagtaacagc aacaacttcc tcatacaagc
     3841 tcctcgctgc gtcaggctcg aattgtactt gaatggccaa gtagtgcgca tcagactcga
     3901 gactcaggga tcgttaacag gcgattttca ccctgaacga aacacgccca gtatcccgac
     3961 gtacatggga tgagttggtg acgacgaggt gcctgagacc catgtccaac acgattttca
     4021 gagggggcgc tctgggacct tctcccgcgt tgccattgac gcgaaccccg attggcccta
     4081 agatggctcg attccaccgg ctgcccggtg accggcggcg cccctgcgag ccatgatcgg
     4141 ccaaactgtc tcccactacc gaatcgtcga aatgctgggc gggggtggaa tgggagtggt
     4201 ctacaaagcc gaagacacac tcctcggccg atttgtcgcc ttgaagtttc tgccggacga
     4261 ttcctcccag gatccacagg cgcttgagcg cttccgccgc gaagcgcgtg ctgcttccgc
     4321 cctcaatcat cccaacatct gcaccatcca cgagatcggc cagcacgacg gccatctttt
     4381 catcgtgatg gagtgcctga gcggccagac gctgaagcac ctgatcaacg gccggccgat
     4441 ggaaatggaa cgcctgctca cgctgtcgac cgaagtggct gacgcgctcg acgcagcgca
     4501 ttcgaagggc atcgttcacc gcgacatcaa gccggccaac atcttcgtca cctcgcaggg
     4561 atacgtcaag cttcttgact tcggcctcgc caagcttacc ggggacactg caaaagcggc
     4621 ggccgcagcg gccagtacgg tgccgaacgc cgacgtcatc gt
//
LOCUS       DASPUL010000074         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_696113, whole genome shotgun sequence.
ACCESSION   DASPUL010000074 DASPUL010000000
VERSION     DASPUL010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696113"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VEJ00_01785"
     CDS             <1..1161
                     /locus_tag="VEJ00_01785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXW99915.1"
                     /translation="KTADIVIVGAGVMGTSIAFHLARRKAGRIVVLDKDHVGRGSSGR
                     SSALIRMHYSFAPEVQLALVSLRMFENWREVAGQPGDFRRTGFIRIVHPNETDRLTRN
                     VEMQRRMGARVDLIDKNTLHELEPDWNLDEVELAAYEPDSGYGDGAGVAGDFLSAARD
                     LGVVYLSRMRSNALKLEGGRVRGVVTDAGIISAATVICATGPWTRPLFRQLGYDPPIE
                     CEYHEVAILRNAPGMKGGGSACIDSVTATYFRSDGQDKFLVGDFYGTRPVDPDNFPQR
                     ASDEGLEKIIERTCRRVPKLAGAEVMRGVTGVYDMTPDSRPLLGEIPGIEGLYLCAGF
                     SGMGFKISPAIGLVMSELVLDGEGRTIDISPFRPDRFARNQPIKAEYEYVDD"
     gene            1180..2181
                     /locus_tag="VEJ00_01790"
     CDS             1180..2181
                     /locus_tag="VEJ00_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HXW99916.1"
                     /translation="MKKKGILERLKEGPVLGDGGYLLELEKRGWVRAGPFTPEVALLY
                     PDALRELHVEFREAGAEVLQALTFYASRDKLATVGLENRLEELNRAAVRVAREVAGEQ
                     CLVAGNLSLTWMYEPGSPSAADRVRKTFDEQLAVQVDEGVDFIIGETFSWLGEALIAV
                     ERAKKTGLPTMVTICFENKDETAEGKSAADAAQALFDAGADIVGMNCLRPPQHILGPM
                     EQMRKAVSGYLACQPVAYRTPEDKPDFTSLPEFPYALDPLQLTRQEMADYAVQARDLG
                     INYIGACCGAVAMHVREMARVLNKLPEDSRIWKKGGAKPMSAYEYYDHDNLKVDTTK"
BASE COUNT          468 a          625 c          691 g          425 t
ORIGIN      
        1 aagaccgccg acatcgtcat tgtcggagcg ggtgtcatgg ggacctccat cgcgttccat
       61 ctggccagga ggaaagctgg acgaattgtt gtgctggaca aagaccacgt cgggcgcggg
      121 agcagcgggc gctcgtcagc attaatccgc atgcactaca gctttgcccc tgaagttcag
      181 ctcgctctcg taagtctgcg gatgtttgag aactggcgtg aagtcgccgg ccagcccgga
      241 gattttcgcc gcaccggatt catccgcatc gtgcatccca atgaaaccga tcgcctcaca
      301 cgcaatgtgg aaatgcaacg cagaatggga gcccgcgttg acttgatcga caagaacacg
      361 ctgcacgaac tggagccaga ctggaaccta gatgaggtgg aattggccgc atacgagcca
      421 gactccggct atggtgacgg ggccggggtt gcgggagact ttctttcggc cgctcgcgat
      481 ctcggagtag tttacctgtc gcgcatgcgc tcaaacgcct tgaaactcga aggcgggcgc
      541 gtccgcggag tggtcacgga tgcgggcatc atctcggctg ctaccgtgat ctgcgcaacc
      601 gggccgtgga caaggccgct tttccggcag ttgggttacg atccgcccat tgaatgcgag
      661 tatcacgagg ttgctatcct gcgcaatgct ccgggcatga aaggcggagg atcggcctgc
      721 atcgactccg tgacggccac ctatttccgc tcggatgggc aggacaagtt tctggtgggc
      781 gatttctacg gaacccggcc ggtcgatccc gacaactttc cccaacgcgc ctctgacgaa
      841 ggattggaga aaatcattga gcgcacttgc cgccgcgttc cgaagctcgc cggtgcggaa
      901 gtaatgcgcg gtgtcaccgg tgtgtacgat atgacgcccg attcgcggcc gctgctgggt
      961 gagattccgg ggattgaggg gctttatctg tgcgccgggt tctccggcat ggggttcaag
     1021 atttctccgg ccattgggtt ggtgatgagc gaacttgtgc tcgacggcga gggcagaacc
     1081 attgatatca gcccatttcg ccccgaccgc ttcgcccgca accagccaat caaggcggag
     1141 tacgaatacg tggatgacta ggaacattca ggagccgtca tgaaaaagaa aggcattctc
     1201 gaacgcttga aagaaggtcc cgtcctgggc gacggcggtt atttgctgga actggaaaaa
     1261 cgcggatggg tgcgcgcagg cccgtttact cccgaggtcg cactattgta tcccgatgca
     1321 ctccgagagt tgcatgtaga gttccgcgag gctggagcag aggtgttgca ggcgctcact
     1381 ttctacgcca gccgcgacaa gctggctacc gttggcctgg agaaccgtct ggaggaacta
     1441 aatcgcgcgg cggttcgcgt cgcccgcgaa gtagcgggcg agcagtgcct ggtcgccggc
     1501 aatctcagcc tgacgtggat gtatgagccg ggcagtccat cagccgccga ccgtgttcgc
     1561 aagactttcg acgagcaact ggcggtgcag gtcgacgagg gagtcgactt catcatcggc
     1621 gaaacattct cgtggctagg cgaggcgctg atcgcggttg agcgagccaa gaaaacaggc
     1681 ctgccgacga tggtgacaat ctgcttcgag aacaaagacg aaacggcgga ggggaagagc
     1741 gccgccgacg ccgcgcaagc attgttcgat gctggagccg atatcgtcgg catgaattgc
     1801 ctgcgtcccc cgcaacacat cctgggcccg atggagcaga tgcgcaaggc agtttccgga
     1861 tatctcgcct gccaaccggt ggcctatcgc actccggagg acaagcctga cttcaccagt
     1921 cttccggaat tcccctatgc gctggatcct cttcagctca cacgccagga gatggcggac
     1981 tacgcagtgc aggcccgcga tttgggaatc aactacatcg gagcatgttg cggcgccgta
     2041 gccatgcatg tccgcgagat ggcgcgcgtc ctgaacaagc tgcccgaaga ttcccgcatc
     2101 tggaaaaaag gcggcgcaaa gccgatgtcg gcctacgaat attacgacca cgataatctg
     2161 aaggtcgaca cgacgaagta gaaaactgca accttcgtga cgcgggtcc
//
LOCUS       DASPUL010000075         5788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_970952, whole genome shotgun sequence.
ACCESSION   DASPUL010000075 DASPUL010000000
VERSION     DASPUL010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970952"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..1319
                     /locus_tag="VEJ00_01795"
     CDS             339..1319
                     /locus_tag="VEJ00_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HXW99917.1"
                     /translation="MLRKTAVFSLLVLVAVSAVAQESRHFTFHYTFTVKNVPTGKKVR
                     IWIPAAQSDAFQQVKVISAKGDLPLMKTGDRKYGDGIYFGETRSAVQHDLHFDVEYDV
                     VRHERIALNAEPHVVAASLTSSERKDDLKPDALVPITGLPAQLAVKVTQGKTAPIDKA
                     RAIYDYVFTTMKYDKTGTGWGRGDVLYACDAKKGNCTDFHSLFIAMARSQGIPARFEI
                     GFPLPADKHEGEIAGYHCWSDFYVEGKGWIPVDISEAWKHPEKRDYFFGSHDVNRVQF
                     SMGRDLRLSPPQKGKALNYFVYPYVEVDNKEYSNVSLAFSFADAATVASR"
     gene            complement(1359..2285)
                     /locus_tag="VEJ00_01800"
     CDS             complement(1359..2285)
                     /locus_tag="VEJ00_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99918.1"
                     /translation="MSKQTRLEIIRDFETQIFYSRTAFPMGAKGLALKDGVTTPNGQE
                     LQQALALWGPAVRPGDPAAITFVQIKDNHIHFDINGGPVHRKKWYQHIEVAGTGGQSV
                     PLSPTQSQPNPHGTYLEVYFDKYVPEMTAQQLRDLLDPVLDFSARNKEEAYLNTVPPK
                     VKEAIQQHRVLVGMNSEMVLHSKGKPPKKVREKEGETQYEEWIYGEPPADVDFVRIIG
                     DEVVRVETMKVDGQKIVRTEKEVVLAKPEEVEAKKQEEDRQPNAPSLRRPGENPDNAP
                     KPSDVGSPTPPMPPPDIPQPPGGGPGVVSGRG"
     gene            complement(2703..3683)
                     /locus_tag="VEJ00_01805"
     CDS             complement(2703..3683)
                     /locus_tag="VEJ00_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99919.1"
                     /translation="MDELAAGRNFKLIAAGAAVGLVYGLAVRLGSRLYPASDLFGVMT
                     VGFMLFLPFAIGFTTIFVIERRQAQPVWIWFLLPWIPVVAGELATLAFFIEGFICVVM
                     FTPIALGASSVGGAAAGLIARLVRSRRSNNATLACVLCLPLLLSSIEPHFPTQHELRM
                     VESVIDIQAPPDTVWRNIERVPRIDPAELQPSWSHTIGFPNPVEATLSAEGVGGVRHA
                     SFEGGVLFIETIDEWEPLRRLGFSIHAQPIPPTTLDEHVTVGGRFFDVLYGEYVLEPL
                     PGGTTRLHLLSRHRVSTDFNWYAHLWTDAVMRDLQKRILQVVKNRAQTGG"
     gene            complement(3790..4350)
                     /locus_tag="VEJ00_01810"
     CDS             complement(3790..4350)
                     /locus_tag="VEJ00_01810"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HXW99920.1"
                     /translation="MARELNFGPYIDIEDGQKVVKDLTYGNPTPEGLILTKLDSAVNW
                     MRKGSIWPMTFGLACCAIEMMSMGASRFDIARFGAEVFRPSPRQSDLMIIAGRVSNKM
                     APVIRQLWEQMPEPKWVISMGACATSGGVFQNYALVQSVNQVIPVDVYVPGCPPRPEQ
                     LIYAITLLQEKIQKERGTIRKALNLV"
     gene            complement(4371..4610)
                     /locus_tag="VEJ00_01815"
     CDS             complement(4371..4610)
                     /locus_tag="VEJ00_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99921.1"
                     /translation="MTKKTETQFVVCVKNKGYSASLELRKLYQAISDERAAKLHQLRI
                     VDESGEDYLYPEGYFVPVQLPQAVERAVRRTTSRG"
     gene            complement(4607..4846)
                     /locus_tag="VEJ00_01820"
     CDS             complement(4607..4846)
                     /locus_tag="VEJ00_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99922.1"
                     /translation="MFFRIVGHISDVETIASGKGIREARRLRRAYGGRRWKKLKGTAV
                     IELADGTMCHAEVHWYEAHGVGAREFKLKRILEAQ"
     gene            5046..>5788
                     /locus_tag="VEJ00_01825"
     CDS             5046..>5788
                     /locus_tag="VEJ00_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXW99923.1"
                     /translation="MPVPTRVNLPGITGPYGHTYADPKPSPDETAFQVDNTSDAYYNS
                     PYYKAHQYQVQPIPRPRMGVPPYLELGDFLPQNLIDTIDQAGEITFHSVGDTGAAKTG
                     PKQSPLVAIQQEAAVADAMTTDVESGGDTGPAFFFHLGDVIYNFGEGQYYYDQFYEPY
                     RDYDRPIFAIPGNHDGMVFGQGSTAPQVPTLDAFLTNFCAGSPGPAPDAPSLARSVMT
                     QPGVYFTLDAPFVSIIGLYSNVLDTGGGI"
BASE COUNT         1160 a         1745 c         1744 g         1139 t
ORIGIN      
        1 gtaggttttg cgcaggtcgc ggcactggat ggcggggggc atgaggagat tccaccggag
       61 caagattgca cgcgtcgtct cgtggaagag cggcgcttca gcgccgcgga cgcggtcggt
      121 cgaacagggc ttcagccccg acaagccgca gggcctaaag gccatttcga gccgtcacaa
      181 aacgcggccc taaagggccg ctcttccacg gtgcggcaga ggttgcccaa cgccgagcgt
      241 ctgcgatgct gctaaaatac cgcaacgccg gaaatcctac cggcagcgcc tgcaaacagg
      301 caccgagagc gctcagcctt ttcgaaagga agccccctat gctgcgtaag actgccgtat
      361 tttctcttct tgtcctcgta gctgtctccg ccgtcgccca ggagtcgcgg catttcacat
      421 ttcactacac gttcacggta aagaacgttc ctacggggaa gaaggtccgc atctggatcc
      481 cggcggcgca gtcggatgcg ttccagcaag tgaaagttat ttcggccaag ggcgatcttc
      541 cactgatgaa aaccggcgat cggaaatatg gcgacggaat ttacttcggc gagaccagga
      601 gcgcggtcca gcacgatctg cactttgatg tggagtacga cgtcgtgcgc cacgaacgga
      661 tcgcgctgaa tgcggagccg cacgtcgtcg cggcctcgct caccagcagc gagcgcaaag
      721 acgacctcaa gcccgatgcc ctggttccga tcaccggcct gccggcccaa ctggcggtca
      781 aagtgacgca gggcaagact gcgcccatcg ataaagcccg cgccatctac gactacgttt
      841 tcacaaccat gaagtacgac aagaccggca cgggatgggg ccgcggcgac gtgctctatg
      901 cctgcgacgc gaagaagggc aactgcaccg acttccattc tctgtttatt gccatggcgc
      961 ggtcgcaggg catcccggcg cggttcgaga tcggcttccc gctcccggcc gacaagcatg
     1021 agggcgaaat tgcgggctac cactgctggt cggacttcta cgtcgagggt aaaggatgga
     1081 ttccggttga tatctccgag gcgtggaagc atccggagaa gcgcgactat ttcttcggat
     1141 cacacgacgt gaaccgcgtg cagttcagca tggggcgcga tctgcggcta agccctccgc
     1201 aaaagggcaa ggccctgaac tactttgtgt atccctatgt ggaagtcgat aacaaggagt
     1261 attcgaacgt gtcgctggcc ttctcgtttg cggacgcggc gactgtcgcg tcgaggtgac
     1321 agcaattgat gtgccttttc tcaggggaag cagcgccctc acccgcggcc gctcacaacg
     1381 cccgggccgc cacctggagg ctgcggaata tcgggcggag gcatgggcgg ggtgggtgat
     1441 ccgacatcgc tgggcttggg cgcgttgtcc ggattctccc cgggacggcg gaggctgggg
     1501 gcattcggtt gccgatcttc ctcctgcttt ttcgcctcca cctcctccgg tttggccagg
     1561 acgacttcct tctcggtgcg gacaattttc tggccgtcga ccttcatggt ctcgacgcgc
     1621 accacttcat cgccaatgat gcggacgaaa tccacatcgg cgggcggttc gccgtagatc
     1681 cactcttcgt attgcgtctc gccctctttt tcgcgcactt tcttcggagg cttgcccttc
     1741 gagtgcagca ccatctccga attcattccc accagcacgc gatgctgctg gatcgcctcc
     1801 ttcaccttgg gcgggacggt gttaaggtag gcttcttcct tgttgcgcgc cgaaaagtcg
     1861 agcacgggat cgagcaagtc gcgcagttgc tgcgcggtca tctcgggaac atacttgtca
     1921 aagtacactt cgagataggt gccatgcgga ttgggctggg actgggtcgg ggaaagcggc
     1981 accgactggc cgccggtgcc ggcaacctcg atgtgctgat accacttctt gcgatggacc
     2041 ggcccgccgt tgatgtcgaa gtgaatgtgg ttgtccttga tctggacaaa cgtgatcgca
     2101 gccggatccc ccgggcgtac tgccggtccc cagagcgcca gcgcctgttg caattcctgc
     2161 ccgttgggcg tggtcacgcc atctttcagc gcgagcccct ttgcgcccat ggggaacgcc
     2221 gtgcgcgagt aaaaaatctg ggtttcgaag tcgcggatga tctcaaggcg cgtttgcttc
     2281 gacatgtgcg cagcattgcg tggaatggga ggagcaacag gagcgtgagg aggcgccggc
     2341 gaattagcct ggcccggtga attgcctagc ggggactgcg tgcgagaagg tttgtccttg
     2401 tcttgccccg tgagcagcag cccggatccc tgaagcgccg cgagcaggac aaccgagcga
     2461 agaagacatc tcataaaccc accccgggcc cgacttgctt attatagcgc cgcattccgc
     2521 gcgctcactc ggggcaatcc agtactttgg gcagtatcgt gcacgaagtc ccaccttgaa
     2581 tttctttgcg tcctttgcgg cagttctttg cgcactttgc gttaaggcct ttcaccgcag
     2641 gggacgcaaa ggacaggccg caaagagtgc agagaaaaac aggcccggca gatgcggggg
     2701 ctttatccgc ctgtctgggc gcggttcttt acaacctgca ggattctctt ctggagatcg
     2761 cgcatcaccg cgtcggtcca caggtgcgcg taccagttga aatcggtgga cacgcggtgg
     2821 cggctgagga gatgaagccg ggtcgtgcct ccgggaagcg gctcgagaac gtattcgccg
     2881 tagagcacgt cgaagaaccg gccgcccacc gtgacatgct cgtcgagcgt ggtcggcggg
     2941 atcggctggg cgtggatgga gaaaccgagg cggcgtagcg gttcccactc gtcgatggtc
     3001 tcaatgaaaa gcacgcctcc ttcgaaactg gcgtgccgga cgccgccgac tccttccgcc
     3061 gacagagttg cctctacagg gttggggaaa ccgatggtat ggctccagga gggttgcagt
     3121 tcggcggggt cgatgcgcgg aacacgctcg atattgcgcc agaccgtgtc aggaggcgcc
     3181 tgaatgtcga tgacgctctc gaccatccgc agctcatgct gggtaggaaa atgcggttcg
     3241 atcgaactga gcagcagagg caggcacagc acgcaggcca gggttgcatt gttcgatcgc
     3301 cgcgaacgca cgaggcgcgc gatcagaccg gcggcggcgc cacccacgct ggatgcgccc
     3361 agcgcgatcg gggtgaacat caccacgcaa atgaagcctt cgatgaaaaa ggccagtgtt
     3421 gccagttcgc cggcgactac ggggatccag ggtaagagaa accaaatcca gaccggttgc
     3481 gcctggcgtc gttcgatgac gaagatcgtc gtaaagccga tggcgaatgg caggaaaagc
     3541 atgaacccga cagtcatcac cccgaacaga tcgctggccg gatacaggcg ggagcccagg
     3601 cgcacggcaa gcccgtagac gagacccacg gccgcgccgg cggcaatcag cttgaagttg
     3661 cggcctgcag ccagctcgtc catgcggcca ctgtacaaca gaaaaccgcc attcgggatt
     3721 taatcgggtt cagccgtttt gaacggaatg aaaaataacg accggcggac gaggcgtccg
     3781 ccgctctact tagaccaggt tgagggcctt cctgatcgtt ccgcgctctt tctggatttt
     3841 ttcctgcagc agtgtgatcg cgtagatcag ctgctcgggg cgcggcgggc agccgggcac
     3901 atagacatcg acgggaatca cctggttcac gctttgcacc agcgcataat tctggaatac
     3961 gcctcccgaa gtcgcgcacg cgcccatcga gatgacccat ttgggctcgg gcatctgctc
     4021 ccagagctgg cggatcacgg gcgccatttt gttcgacacg cggccggcga tgatcatcag
     4081 atcgctctgg cgcggggagg ggcggaagac ttcggcgccg aaacgggcaa tgtcgaagcg
     4141 cgaggcgccc atcgacatca tttcgatggc gcagcaggcc aagccgaaag tcatgggcca
     4201 gatcgaccct ttgcgcatcc agttgacagc cgagtccagc ttagtcagga tgagcccctc
     4261 aggcgttgga ttgccgtacg tcagatcctt gacgaccttc tggccgtctt cgatgtcaat
     4321 gtacgggccg aagttcagtt cgcgggccat agaaatattt taaaccagga ttagcctcgg
     4381 ctggtggtgc gccgaactgc cctttcgaca gcttgaggaa gttgaacggg gacaaaatag
     4441 ccctcgggat agagatagtc ttcgcccgat tcgtcgacga ttcggagttg gtgcaatttg
     4501 gcggcgcgct cgtcagaaat ggcctgatag agcttgcgca gctcaagcga cgccgagtat
     4561 cccttgttct taacgcaaac cacgaactgc gtctcggttt ttttcgtcat tgagcttcca
     4621 gaatgcgttt gagcttgaac tccctagcgc cgacgccgtg ggcttcgtac cagtgcacct
     4681 cggcatgaca catggtacca tccgccagtt cgatgacagc ggttcccttt agcttcttcc
     4741 aacggcgacc gccataggct ctgcggagcc ggcgcgcttc acgaatgcct ttgccagaag
     4801 cgattgtctc tacgtctgaa atatgaccaa cgatcctgaa gaacacgagg cgattgtatc
     4861 ctcaatgaag aacgatctct cttgacaatc gtttcctatg cgctaagctg tcacgcatct
     4921 tcccctcctg aaactaggcc ctgttcgtga ccctgtaact ctatctggga gccccgaaat
     4981 caacccgcgc cgatgcggga gaaagcatca tgggagcagt ctctgctccc tgagaggatc
     5041 agcctatgcc agttccaacc agagtcaatt tgcccggtat cacgggccct tatggccaca
     5101 cctatgccga tcccaaaccc agtccggacg aaactgcatt tcaggtcgac aacaccagcg
     5161 acgcgtacta caactcgccg tactacaagg cgcaccagta ccaggtgcaa ccgattccgc
     5221 gcccgcggat gggagtgccg ccttacctcg agctcggcga cttccttccg caaaacctga
     5281 tcgacaccat cgaccaggcg ggagaaatta cgtttcactc cgtgggagac acgggcgcgg
     5341 ccaagaccgg ccccaagcaa tcgccgctgg tcgccattca acaggaggct gccgtcgccg
     5401 atgccatgac cactgacgtt gagtccggcg gcgataccgg gccggcgttt ttctttcacc
     5461 tgggcgacgt gatctacaac ttcggcgagg gccagtacta ttacgaccag ttctacgagc
     5521 cgtacagaga ttacgaccgg ccgatcttcg ccatccccgg caatcacgac ggcatggtgt
     5581 tcgggcaggg atcgaccgcc ccgcaggtcc ccacgctcga cgcgtttctc acgaacttct
     5641 gcgcaggctc gcccgggccg gcgccggacg cgccttcgct cgcgcgcagc gtgatgaccc
     5701 agcctggcgt ttacttcacg ctcgatgccc cgttcgtctc gattatcggc ctgtacagca
     5761 acgtgctcga caccggtggc ggcatcat
//
LOCUS       DASPUL010000076         4475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_971067, whole genome shotgun sequence.
ACCESSION   DASPUL010000076 DASPUL010000000
VERSION     DASPUL010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971067"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            486..3791
                     /locus_tag="VEJ00_01830"
     CDS             486..3791
                     /locus_tag="VEJ00_01830"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXW99924.1"
                     /translation="MKVRVILTCVVLMFIVAAAVSLSAQTFRGTILGTVTDASGAVVA
                     GAKVTVRNSGTGLERSTETSAEGTYSVPELPIGTYTVTITQPGFQTYVAKDVVVDVSA
                     EHRVDAKLITGQVSQSVEVNAEIPLIETTSNTLGGTIGAADIANLPINGRDFVKLATL
                     VPGAGADANGDSDSPGALGTFSVNGNRGRANNYLIDGTDMNDGFRNDPAINEGGVFGT
                     PATLLPTDAVAEAGLLSNMEAEYGRNSGGVMNIVTKSGTNFLHGSVFEYFRNSGLDAR
                     NYFNCAVSSAFCTAEPQDAFHNNQFGGSLGGAIVKDKTFFFLSYEGWRERGGIPTTAN
                     VPTQAEVSAFGVNNVNPVITSLLARNPWSIPLPAGTDAVTPATVQVTDPFSNRVDSLI
                     VKLDQHLGTGDLLTGRYYFGDSDQSFPLGLGGGTTVPGYNSTTPTRVQVVSLSYTHVW
                     SPKMVMEARGGWVRFAEGFFPQDGKFDPNTIGMDTGVTGHPVDYGLPQIAFSDGTSNL
                     GGSGSFPRQRYDSNWQYFTNLSYNTGKHSIKFGYEFRRSTINQIYDLGFRGKLKFTSF
                     DAFLAGTPDNGGSQFGGDSHRITHENNDGFYIQDNYRITPRLTLNLGMRWDYFGVIYA
                     QNHDFSLFDAATDSLQQVGTSGAPSSLYPKDLNNFAPRFSAAYDVFGSGKTVVRAGWG
                     LFYDAFAQDLFEGHAPFNTFNAGPAYNGIGPQGIQSAGSVAGAIQSGVPIFTDFAPTG
                     DVWTVDQRLRTPYVYNYNVNVEQSVGRNMAFTVGYVGSQGVKLFRFVDLNQHDPATGA
                     FPFANFNIINQTQTTAISHYNSLQATWNLRNLHGINSQLVYTWSHSIDTASDEDDNVP
                     NAAQPDNSLNPAAEKANSSFDARNRLIWTLNYALPNSSRVRILTNGWTVDGLLHITSG
                     EPYNIVSFSNYNNTNEFLERPDVVGNPFAGVHAPYQYLNLTALAAPCDWDPVAAACIA
                     GTQHFGNLRRNAFTGPAFHDFDFSLAKNTKLGERVNMELRADFFNIFNHPNFGNPLMP
                     NFSVNLENNGTVTPLSFTGPCSTAPGTDHTGCRAVGPGFLPIAGTPDVVAGEPFIGGG
                     GPRNIQLAVKFTF"
     gene            3999..>4475
                     /locus_tag="VEJ00_01835"
     CDS             3999..>4475
                     /locus_tag="VEJ00_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99925.1"
                     /translation="MRRSNCFQAAVLILGLCLCVGMAAPAFAHHLKKGETATLPITTS
                     SAKARQLYQKGMADYENLYLERCNDDWRAAVKEDPNLAVAWAWIAFNSSNPQEVSAAR
                     AKAKELAPKLTPGEQLMVAWISKVQEGDYLGGITAMNDMLEMYPRDKHLLYLAGNWL"
BASE COUNT          932 a         1304 c         1344 g          895 t
ORIGIN      
        1 tcctcgatgt gcgcaacctg ctggcccagg gctatgttcc ggtcctcggg caggatggac
       61 agacagtgta cctggtgcaa tcggcgcgag ccgtgcgcgg cggactggcg ttcacgttct
      121 agctcgccag ccgcactctc gtgctcgatc ccgacgttgt aaacctcagc ttccattcgc
      181 ttatcttttg tccggcgcga cagccgctgc gaatgtcgcg gacgatggcc gtgcggcgag
      241 gtgcgcgacg gcagactgta attgtgagcg ctctcattca cgaaatcgat cacaacgatt
      301 gtcaattccg tggttgactc tggcgagttg gtttcgataa tgttgctcgc taagcaatag
      361 accctcggcg gcgcatcttg ttcgcaggga agaaattgcg cacgctccac gagatgatcg
      421 aaaaccgtct ggccagtccg ctacgaacca ccccgaaccc agcacatcac cctgaaggag
      481 agcccatgaa agtacgcgtg atcctgactt gcgtcgtttt gatgtttatc gttgcggccg
      541 cggtgagcct gtcggcgcaa acattccggg gaaccattct ggggacagtg accgatgcct
      601 cgggggcagt ggtggccggg gcaaaggtga cggtgaggaa cagcggcacc ggacttgagc
      661 ggagcaccga aaccagcgct gaaggtacgt attctgtgcc ggaactgccc atcgggacgt
      721 acacggtgac gattacgcag cctggatttc agacctatgt ggcgaaggat gtagtcgtgg
      781 atgtctccgc cgaacaccgg gtggatgcga agctgatcac tggacaggtg tcgcagagcg
      841 tggaagtgaa tgcagagatt cccctgattg agacaaccag caacacgctt ggagggacaa
      901 tcggggccgc tgacatagcc aatcttccga tcaacgggcg tgatttcgtc aaactggcga
      961 ctctcgtccc gggagcgggc gctgacgcta atggcgacag cgactctccg ggtgctctgg
     1021 gaacgttcag tgtgaacggc aatcggggcc gcgcgaacaa ttacctgatc gacggcaccg
     1081 acatgaatga cggattccgc aacgacccag cgatcaatga gggtggcgtc ttcggaacgc
     1141 ccgccacgct gttgcctact gacgctgtgg cggaggcggg cctgctctcc aacatggagg
     1201 cggaatacgg gcggaactcg ggcggcgtga tgaatatcgt gaccaagtcg gggacgaact
     1261 ttctacacgg ttcagtcttc gagtatttcc gaaacagcgg cctcgatgcc aggaactatt
     1321 tcaactgtgc ggttagttct gcattctgta cggcggagcc gcaagacgcc ttccacaaca
     1381 accagttcgg aggatcatta ggcggggcga tcgtcaaaga caagacgttc ttctttctct
     1441 cctatgaggg ctggcgcgag cgcgggggga ttccgactac cgcaaatgtt ccaactcagg
     1501 cagaagtgag tgcatttggg gtaaataacg tgaacccggt cattacgagc ctgctggccc
     1561 gcaacccgtg gagcatccct ctgccggcgg gaacggatgc tgtgactccg gccacggtgc
     1621 aggtcacgga tcccttcagc aaccgtgtag acagcctcat cgtcaagctg gatcagcacc
     1681 tggggaccgg ggatcttctg accgggcggt actacttcgg ggacagtgac cagagcttcc
     1741 cgctgggctt gggcggtgga accaccgtcc ccggatacaa cagcacgacg ccgacgcgcg
     1801 tgcaggtggt ttctctgtcg tacacccacg tgtggtctcc caagatggtc atggaggcgc
     1861 gcggcggctg ggtgaggttt gccgagggct tctttcccca ggacggcaag ttcgacccga
     1921 acacaatcgg gatggatacc ggtgtgacgg ggcatccggt ggactacggc ctaccgcaga
     1981 ttgctttctc ggacggtacc agcaacctgg gcgggagcgg atcgttcccc cgccagcgct
     2041 atgattcgaa ctggcagtat ttcacaaatc tctcctacaa cacgggcaag cacagcatca
     2101 agttcgggta cgagttccgg cggtcaacga tcaaccagat ctatgacttg ggattccgcg
     2161 gaaagctgaa gttcactagc ttcgatgctt ttctcgcagg tacaccggac aacggcggga
     2221 gccagttcgg gggagactct caccgcatca cgcatgagaa taacgatggc ttctacatcc
     2281 aggacaacta ccgtatcacc ccgagattga ctctgaatct gggaatgcgc tgggactact
     2341 ttggcgtgat ctatgcgcag aaccacgact tcagcctgtt cgacgctgcg accgactcgc
     2401 tgcagcaggt gggaacgtcg ggtgccccat catcgctcta cccgaaggac ctgaacaact
     2461 ttgcgccacg tttcagcgct gcctacgacg tgttcgggtc cggcaagacg gttgtccggg
     2521 ccgggtgggg actgttctac gacgcttttg cgcaagatct gtttgagggg catgcgccat
     2581 tcaatacctt caatgcgggt cctgcataca acggtatcgg gccccagggg atccagtccg
     2641 cgggctccgt tgccggcgcc atccagagtg gcgtgccgat ctttacggac ttcgcgccga
     2701 cgggcgacgt ttggacggtc gaccagcgat tgcgaacgcc ctacgtctac aactacaacg
     2761 taaacgtcga acagtcggta ggcaggaaca tggcgttcac cgtgggctac gtgggctcgc
     2821 aaggcgtcaa actgtttcgc tttgtcgatc tcaatcagca tgatccggcg acgggagcct
     2881 tcccgtttgc caacttcaac atcatcaatc agacccagac gacggcgatc tcccactaca
     2941 acagcctgca ggccacatgg aacctgcgca acctgcacgg catcaactcg cagttggtgt
     3001 atacatggtc gcactcgatc gacacagcga gcgacgagga cgacaatgtg ccgaacgccg
     3061 cgcagccaga caacagcttg aacccggcgg ccgagaaggc aaactccagc tttgatgcgc
     3121 gcaatcgcct gatctggacg ctgaactacg cgctgccgaa ctcatcccga gtccggatac
     3181 tgaccaatgg ctggacggtc gatgggctgt tgcacatcac gagcggcgaa ccatacaaca
     3241 tcgtgtcgtt ttccaactac aacaacacta acgagttcct ggagcggcca gatgtcgtgg
     3301 ggaatccatt tgcaggagta catgcgccgt accaatatct caatctgacg gccctggccg
     3361 cgccttgcga ctgggatccg gtggctgctg cctgcatcgc tggaacgcag cacttcggga
     3421 acctgcggcg gaacgccttt accggacctg ctttccatga cttcgacttc tccctcgcca
     3481 agaacacgaa actgggagag agggtcaaca tggagttgcg cgcggacttc ttcaacatct
     3541 ttaatcaccc gaattttggg aatcccctta tgcccaactt ctccgtgaac ctggagaaca
     3601 atggcacggt gaccccacta agctttacgg gtccgtgcag cacggctccc gggacagatc
     3661 acaccggatg ccgcgccgtc ggtcctggct tcctcccgat cgcgggcacg ccggacgtgg
     3721 tggcgggtga gccgttcatc ggaggcggag gaccacggaa catccaactg gcggtgaagt
     3781 tcactttcta attcccagca cctgtgacga acgcccctgc cggcttaggg caggggcttt
     3841 tgttttccgc cacgagccgg ggcgaggggt gcgaggtcgg catcgattcc gcatgcgcac
     3901 tcggccactg cctttcgtca cctcgtgccg cgattactgc gatgttacgc tgtgcgggta
     3961 agcctcccca taaagctctg ttccggagaa aatcccgcat gcgtcgctcc aattgctttc
     4021 aggctgcagt cctgattctt ggtctttgtc tttgtgttgg catggctgcg ccggctttcg
     4081 cgcaccatct caagaaaggt gaaactgcca cgctgccgat cacgacctcg tcggccaaag
     4141 cgcgccagct ctatcagaag ggcatggccg actacgagaa tctctatctg gagcgctgca
     4201 acgacgattg gcgggcggca gtgaaagaag atccgaatct tgctgtagcc tgggcctgga
     4261 ttgcgttcaa cagcagcaat ccgcaggaag tcagtgcggc tcgggccaag gccaaggaac
     4321 tggcgcccaa gctgacgccg ggcgagcagt tgatggtggc ctggatttcg aaggtgcagg
     4381 aaggcgacta cctgggcggc atcacggcga tgaacgacat gctggagatg tatccgcgcg
     4441 acaagcacct gttgtatctg gccgggaact ggctg
//
LOCUS       DASPUL010000077         3144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1108441, whole genome shotgun sequence.
ACCESSION   DASPUL010000077 DASPUL010000000
VERSION     DASPUL010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108441"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VEJ00_01840"
     CDS             <1..586
                     /locus_tag="VEJ00_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99926.1"
                     /translation="LVVYPPQSSTFAPEENIIIGNVALYGATSGEAFFNGVAGERFAV
                     RNSGATAVVEGVGDHGCEYMTNGLVVVLGSCGRNFAAGMSGGIAYVFDERGGFTEKRC
                     NLESVDLEPLLDDRDMQIVRDLVARHAELTRSPRAKFVLDNWDGVLPRFIKVFPHEYK
                     RVQGISRSQQAYSPSQMAFMPDTEPLDAEQVQHG"
     gene            579..2051
                     /locus_tag="VEJ00_01845"
     CDS             579..2051
                     /locus_tag="VEJ00_01845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HXW99927.1"
                     /translation="MGKTTGFMDYSRELAPRRPVAERVNDWFEIYQDFPEDKLRQQGA
                     RCMDCGVPFCQTGCPVNNLIPDWNDLVFRGRWKEALRQLHATNNFPEFTGRICPAPCE
                     AACVLGINQPPVTIKQNEKNIVERGFLEGWIRPEPAKVRTGKKVAIVGSGPAGLAAAQ
                     QLCRAGHSVVVYEKADRIGGLLRYGIPEFKLEKHIIDRRLEQMSSEGVKFVTNAEVGK
                     NVAVEELRRDFDAIVLAGGSEQPRDLKVPGRELRGIHFAMEFLPQQNKRCLGDVIDPA
                     AEILATGKRVVIIGGGDTGADCLGTCHRQKPLSVRQFEIMPKPPEERSPQTPWPLWPM
                     QLRTEAAHEEGGMRDWGIATTKFTGDDNRQVRRLYAVRVGPPPKFAPIAGSEFTMDAD
                     LVLLAMGFAGPVRNGMIEQLGVQLDDRGNVATDQNYMTSVPGVFAAGDMRRGQSLVVW
                     AISEGRKAAACVDTYLARERAQHLINQATQGASVSTPATI"
     gene            2104..>3144
                     /locus_tag="VEJ00_01850"
     CDS             2104..>3144
                     /locus_tag="VEJ00_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXW99928.1"
                     /translation="MGKADHPSLAIGTDRQSNGNIAGAQPPIETDSIQITPPARVAAG
                     LKSVLKAAEVTLKEPGLVRGVAALSRLNQFDGIDCPGCAWPDPDHERSLNEYCENGVK
                     AIAEEATAKRCSPDFFRSWSVAELAAQSDYWLGHQGRLTDPMVLREGRDHYEPISWDD
                     AFTMIAGELNSLSSPNQAIFYTSGRTSNEAAFLYQLFVRQFGTNNLPDCSNMCHESSG
                     LALKEVIGVGKGTVKLEDFDLADTILIIGQNPGTNHPRMLATLQKAARRGAAIVAVNP
                     LAEVGLTRFKHPKDVLGLFGAGTKIATHFVQVRLSGDLAFFQGLCKELLEEEARRPGE
                     VIHHDFVQQKTTG"
BASE COUNT          631 a          915 c          988 g          610 t
ORIGIN      
        1 gctggtggtg tatccgccgc agagttcgac tttcgctccc gaagaaaaca tcatcatcgg
       61 caatgttgcg ctttacggcg ccacgagcgg cgaagcgttc ttcaacgggg tagccggaga
      121 gcgctttgcc gttcgcaatt ccggcgcgac ggcagtggtc gagggggtcg gcgaccatgg
      181 ctgcgaatac atgaccaacg gcctggtggt cgtgctcggc tcctgtggca gaaacttcgc
      241 ggcgggcatg agcggtggca ttgcatacgt ctttgacgag cgcggaggct tcaccgaaaa
      301 acgctgcaac ctggaatccg tagatctgga gcccctgctt gatgaccgcg acatgcagat
      361 tgttcgcgat ctcgtcgcgc gccacgcgga gctgacccgc agtccgcgcg cgaagttcgt
      421 cctcgacaac tgggacgggg tgcttccacg ctttatcaaa gttttcccgc acgaatacaa
      481 gcgggtccag ggaatcagcc gaagccaaca agcgtacagt cccagccaaa tggccttcat
      541 gcccgatacc gaaccgttgg atgctgagca ggtgcagcat gggtaagacc acaggcttca
      601 tggattattc gcgcgaactg gccccgcgtc ggccggttgc tgagcgcgtc aacgactggt
      661 tcgagatcta tcaggatttt cccgaggaca agctgcgcca gcagggggcg cgctgtatgg
      721 attgcggcgt gcctttctgc cagacgggat gccccgtgaa caatctcatt cccgattgga
      781 acgatcttgt ttttcgcggg cggtggaagg aagcgcttcg ccagctgcac gccacgaata
      841 actttccgga gtttaccgga cgcatttgcc cggcgccttg cgaagccgcg tgcgtcctgg
      901 ggatcaacca gccgccggtg acgatcaagc agaacgagaa gaatattgtg gagcgtgggt
      961 tcctcgaagg ctggatccgc cctgagcctg caaaggttcg cacgggcaag aaagtcgcga
     1021 tcgtgggctc ggggccggca ggactggccg ctgctcagca actctgccgc gccggacatt
     1081 cggttgtggt ctatgaaaaa gccgaccgca tcggcgggct tcttcgctac ggcatcccgg
     1141 agttcaagct ggagaagcac attatcgacc ggcggctcga gcagatgtcc agcgaaggtg
     1201 tgaaattcgt taccaatgcc gaggtaggca agaacgtcgc tgtggaagag ttgcggcgcg
     1261 atttcgatgc catcgtgctg gcgggcggtt cagagcagcc gcgggatttg aaagttccgg
     1321 ggcgcgagct gaggggaatt catttcgcga tggagtttct cccgcagcag aacaagcgct
     1381 gcctcggcga tgtgattgat ccggcggctg agattctcgc gaccggcaag cgcgtggtga
     1441 tcattggcgg aggcgacacg ggagccgact gcctgggcac gtgtcatcgc cagaaaccgc
     1501 tttccgttcg ccagttcgaa atcatgccga agcctcccga ggagcgttcc ccgcagacgc
     1561 cgtggccgct ttggccgatg cagcttcgca ccgaggccgc tcacgaggaa ggtggcatgc
     1621 gcgattgggg cattgccaca acaaaattca ccggcgacga caaccgacag gtcagacgac
     1681 tttacgccgt aagggtcggc cctcccccga agtttgcgcc gatcgcgggc agcgagttca
     1741 ctatggacgc cgatctcgtt ttgctcgcca tgggtttcgc gggcccggtg cgcaacggga
     1801 tgatcgaaca gctgggcgtc cagctcgacg accggggcaa cgtcgcgacg gatcagaatt
     1861 acatgacgtc agttccggga gtgtttgcgg cgggcgacat gcgacgcggg cagtcgctgg
     1921 tggtctgggc catctccgag ggtcgcaagg cggctgcttg tgtcgacacc tatcttgcgc
     1981 gtgagcgcgc tcagcatctc atcaaccaag ccactcaagg ggcctctgtt tccactccag
     2041 ctacgatctg aatacctccg ttcttgggat cctccattgt cgtagactgg tctgggggat
     2101 tctgtgggga aagcggatca tcccagcctg gcgatcggaa ctgatcgtca gagcaacggg
     2161 aacatcgcgg gggcccagcc tccgatcgaa accgactcca tccaaatcac tcctcccgca
     2221 cgcgtcgcag cgggactgaa gtccgtcctc aaagcagctg aggtcaccct aaaagagcca
     2281 gggctggtga gaggcgttgc cgcgctgtcc cgtctcaatc agtttgacgg aatcgactgt
     2341 ccgggctgtg cctggcccga tcctgatcac gagcgatcgc ttaatgaata ctgcgagaac
     2401 ggcgtcaagg cgattgcgga ggaggccacg gccaagcgct gctcgccgga tttctttcgc
     2461 tcgtggagtg tggctgaact ggccgcgcag tccgactact ggctggggca tcagggcagg
     2521 ctgactgatc ccatggtttt gcgggaaggc cgcgaccact atgaaccgat ttcctgggac
     2581 gatgccttca ccatgatcgc gggtgaactg aattcgctga gcagtccgaa tcaggccatc
     2641 ttctacactt ccgggcgcac cagcaacgag gcggcattcc tctatcaact gtttgtgcga
     2701 cagttcggca ccaacaacct gccggactgc tccaacatgt gccatgagtc gtccggtctg
     2761 gcgttaaagg aggtcatcgg cgtcggcaag ggaacggtca agctggagga cttcgatctc
     2821 gcggatacca tccttattat tggtcagaat cccgggacca atcatccacg catgctggct
     2881 acgctgcaaa aagcggcgcg gcggggcgcg gccatcgtcg ccgtgaaccc cctggcagaa
     2941 gtggggttaa cgcgattcaa acaccccaag gatgtgctcg gcctgtttgg agcgggcacc
     3001 aaaatcgcga cccatttcgt tcaagttcgc ctcagcggtg acctggcgtt cttccaaggc
     3061 ctctgcaagg aacttctgga agaagaagcg cggcggccag gggaagtgat ccatcacgac
     3121 ttcgtccagc aaaaaaccac aggc
//
LOCUS       DASPUL010000078         4663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1793160, whole genome shotgun sequence.
ACCESSION   DASPUL010000078 DASPUL010000000
VERSION     DASPUL010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793160"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..870
                     /locus_tag="VEJ00_01855"
     CDS             235..870
                     /locus_tag="VEJ00_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXW99929.1"
                     /translation="MSGKRVFIATVAALALLTTLAAAQDEKNEVGGTVGRTFVSDQGI
                     KLNIADPTIHSGKGVTLEGFFAHRFLVTPIYSIAGEVPVAYNVDEDLVAGQIGNSVPA
                     DYKALFVTPAARVNLFPTTAVSPWVSFGAGFGHFSEGKTLAFGGANPGKSTTNFVLEG
                     GLGLDVRVWSRLSIRGQVRDFWSGEPDFPQAPTGKSRQHNFFVSGGAFWRF"
     gene            902..1726
                     /locus_tag="VEJ00_01860"
     CDS             902..1726
                     /locus_tag="VEJ00_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXW99930.1"
                     /translation="MKIFLSGLLLAVVLASSLCPGQSQSPPPSHPQEVVFPSGGLQLH
                     GFLWKPDGAGPFPAIVWNHGSEKLPGSQPGLGAYYTAHGYVFFVPHRRGQGRSPGSYI
                     QDLVAEAPPRARAERMVELQQAEVVDVVAAVNYIKAQPFVDGGRIAISGCSFGGIQTL
                     LAGEQDLGVKALIPFAPGAMSWEQNLPLHDLLLRAIDSARAPVFLIQAENDYDLAPSH
                     ALSKEASKKGKDLQSKIYPVFGKGHQEGHWGFCTAGMDVWGNDVLTFLEARMKAGQ"
     gene            complement(1754..3973)
                     /locus_tag="VEJ00_01865"
     CDS             complement(1754..3973)
                     /locus_tag="VEJ00_01865"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HXW99931.1"
                     /translation="MSAASAQTPAQVAASGAMPLARAIDHYFEVALYLLVLAGFGTLA
                     STGGLDLPSMLLTGAALAVRGYLLARRRQLVISEGWTTPLTFFYFIFFAVDYLVFSRS
                     FLPATVHLALFAVVVRTFSLRRDRDHVMLAILAFLMVLASAVLTVDSVFLLSFAGFML
                     MAVGTFVLMEMRRSGRDASVLARHSHDPQEHRHLAFSLARVMPALMILILVAGAALFF
                     LLPRMSAGYLGGYSFGTDFSSGFSDHVQLGRIGEIQQSNAVVMHIQIDGDTVGSYDLH
                     WRGISLASFDGRTWSNPRELGVRLQPQSANSFAVPRVGNQVERFDATGTPPRPNLIHY
                     RVLMEPIGTNVFFLAPWPRAITGEYRRMDADSGGAIYDLDPRHAISRYEADSDISRPS
                     AGELRAAGSDYPPKIANVYLSLPPLDQRVPRLAQLIAASATNNFDKAAVLENYLRTRY
                     AYTLQLPQTSPDDPIANFLFVRKQGHCEYFAASMAVMLRTLGIPSRVVNGFRTDEFND
                     LTGNYVVRAKDAHAWVEAYFPGYGWQTFDPTPGGSANTPQGWGRVGLYVDAMASFWRE
                     WIVSYDSSHQYVLGQAAVSGTRNTWEEARAWARRHYESMLQWARRSQERLEQSPGKGI
                     SLGAAIILGLLLFGNLGRIARLLHEKWLQAHPERSPDQAAAMWYEKMARALAKRGVAK
                     AAAQTPQEFVNRIENPRLREPVARFTHVYESARFGNSADDVQKLPELYELVENATRE"
     gene            4171..4267
                     /gene="ffs"
                     /locus_tag="VEJ00_01870"
     ncRNA           4171..4267
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VEJ00_01870"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(4465..>4663)
                     /locus_tag="VEJ00_01875"
     CDS             complement(4465..>4663)
                     /locus_tag="VEJ00_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99932.1"
                     /translation="PFLYMPGETDLYFPVGDARYEAGFMSTVTLKPIPSLWGHTAGAA
                     GSAADGKFLNENISAFLMEKR"
BASE COUNT          904 a         1357 c         1530 g          872 t
ORIGIN      
        1 ccggggtcgg gttcgagaat tgtcctgagg cgaagcggtg atcgtggagc ggtccaactt
       61 catggggtgt tcccatctta gcatccaacg gtccgcgatc ctgcatcgca gcgcgtggcc
      121 ccggccccgg ccaggatcat ataaagatat acttgctggg ttcaaaaccc cggctggcgt
      181 gctaggctat gccttaccgc cgtaatgaca accaggtgcc tcgcaggagg agaaatgtct
      241 gggaaacgag tgttcattgc gaccgtcgcc gcactggcgc tgttaactac cttggcggcc
      301 gcccaggatg agaagaacga ggtcggcggc accgtcggtc ggacttttgt cagcgaccaa
      361 ggcatcaagc tgaatatcgc cgaccccacc atccattccg gcaagggtgt caccctggag
      421 gggtttttcg cgcatcggtt cctggtcacg ccgatttatt cgatagcggg agaagtgccg
      481 gtggcgtata acgtcgacga agacctggtt gccggtcaga tcggcaattc ggtacccgcc
      541 gactacaagg ctctgtttgt cactcctgca gctcgcgtga acctgtttcc caccacggcg
      601 gtttccccct gggtaagttt cggcgccggc ttcgggcact tcagcgaggg caagacgctc
      661 gcgttcggag gcgcgaatcc cggcaagtcg accacgaact tcgtcctcga agggggtttg
      721 ggcctggatg tgagggtctg gagtcgccta agcatccgcg gccaggtgcg cgacttctgg
      781 tccggcgagc cggactttcc ccaggctcca accgggaaat cccgccagca caacttcttc
      841 gtgagtggcg gagctttctg gcggttctag gctgcctgca ggcccgcgag cacaatcggg
      901 catgaagata ttcctcagcg ggttgctgct ggccgtggtg cttgcgtcga gtctctgccc
      961 cggtcaatcg caatccccac ccccatccca tccccaagag gtagtttttc ccagcggcgg
     1021 actgcagtta cacggatttc tgtggaagcc cgacggcgcc ggaccgtttc cggcgattgt
     1081 ctggaaccac ggcagcgaga agctgccggg gtcgcagccc gggctgggag cgtactatac
     1141 agcgcacggc tatgtgtttt tcgttccgca ccggcgcggc caggggcgct ctccgggcag
     1201 ctatattcaa gacctggtcg ccgaggcgcc accgcgtgcg cgcgctgagc gcatggtcga
     1261 actgcagcag gctgaagtcg tggacgtcgt tgccgccgtg aactatatca aggcgcagcc
     1321 gttcgtagat ggcggccgca tcgccatctc aggatgttcg tttggaggaa tccagaccct
     1381 gcttgccggt gagcaggatc tcggagtcaa agcgctgatt ccgttcgctc ccggcgccat
     1441 gtcgtgggag cagaacctgc cgctgcacga cctgcttctt cgcgccatag attccgctcg
     1501 cgcaccggtc ttcttgattc aggcggagaa cgactacgac ctggctccga gccatgcctt
     1561 gagcaaagag gcgagcaaga aggggaagga tctgcaatcg aagatctatc ctgtgttcgg
     1621 caagggccat caggaaggac attggggatt ctgtaccgcg ggaatggatg tttggggcaa
     1681 cgacgtgctg acgtttctgg aagcgagaat gaaggctggc cagtagggtt gtggaagcga
     1741 gcgaggccga cggctattcc cgcgtcgcgt tttccacgag ttcgtagagt tcaggcaatt
     1801 tctggacgtc gtcggcggag tttccgaagc gcgccgattc atagacatgg gtaaaacgcg
     1861 cgaccggctc gcgcaggcgc gggttttcga tccggttgac gaattcctga ggcgtttgcg
     1921 cggcggcctt ggctacgcca cgcttcgcca gcgcgcgggc catcttctca taccacatcg
     1981 cggcggcctg gtccggggag cgctcggggt gcgcctgcaa ccatttctcg tgcagcaggc
     2041 gggcgatccg gcccagattt ccaaataaca ggagccccag gatgatggcg gcgccgaggg
     2101 atattccttt gccgggggat tgttcgagtc gctcctggct gcgccgcgcc cattgcagca
     2161 tggattcgta gtgacggcga gcccaggcac gcgcctcttc ccaggtgttg cgggttccgc
     2221 tgaccgcggc ctggccgagc acgtactggt gcgagctgtc gtagctgaca atccactcgc
     2281 gccagaacga ggccatggca tcgacgtaaa gaccgacccg gccccagccc tgcggcgtgt
     2341 tggcgctgcc gccgggtgtg ggatcgaacg tctgccagcc gtagccgggg aagtaggctt
     2401 ccacccaggc atgggcgtcc tttgcccgga cgacgtagtt tccggtgagg tcgttgaact
     2461 catcggtgcg gaagccattc accacgcgcg acggaattcc cagggtgcgc aacatcacgg
     2521 ccatggaagc ggcgaaatat tcgcagtgtc cctgcttccg gacaaacagg aagttggcga
     2581 tgggatcgtc cggagatgtt tgcgggagct gcagcgtata ggcgtagcgg gtacgcaggt
     2641 agttctcgag tacggctgcc ttatcgaaat tgttcgtggc cgatgcagcg atcagctgcg
     2701 ccagtcgcgg cacgcgctgg tccagaggag gaaggctcag atagacgttt gcgatcttcg
     2761 ggggatagtc gcttccggcg gcgcgcaatt cgcccgcgga aggcctcgag atgtcggaat
     2821 cggcttcgta gcggctgatg gcgtggcgcg gatcgaggtc gtagatagcg ccaccggaat
     2881 cggcgtccat gcggcggtac tcgccggtga tagcgcgagg ccacggcgcc agaaaaaaga
     2941 cgttggtgcc gatgggctcc atcagcacgc gatagtgaat gaggttaggg cgggggggcg
     3001 tgccggtcgc atcaaagcgc tcgacctggt tgcccacgcg cgggacggca aagctgttgg
     3061 ccgattgcgg ctgcaacctc acaccgagct ctcgcgggtt ggaccacgtg cggccgtcaa
     3121 agcttgccag cgagatgccg cgccaatgca gatcgtagct gccgaccgtg tcgccatcga
     3181 tctggatgtg catgaccaca gcgttcgact gctgaatctc gccgatgcga ccgagctgga
     3241 cgtggtcgct gaagccggag gaaaaatcgg tgccgaagga atatccgccg aggtagccgg
     3301 ccgacatgcg cggcagcagg aagaacagtg cggcgccggc gaccagaatg agaatcatca
     3361 aggcgggcat gacgcgcgcc agcgaaaacg cgagatgccg gtgctcctgc gggtcgtgcg
     3421 agtggcgcgc gagcacgctg gcatcgcgtc cggagcggcg catttccatc agcacgaacg
     3481 tgccgacggc catcagcatg aagccggcaa aggaaagcaa gaacacgctg tcgacggtga
     3541 gcacggccga ggccagcacc atcaggaagg cgaggatggc cagcatgacg tgatcgcggt
     3601 ctcggcgtag agagaaagtg cgtacgacta cggcaaacaa cgccaggtga actgtggccg
     3661 gcagaaagct gcgggagaag acaaggtagt cgacggcgaa gaagatgaag tagaagaagg
     3721 tgagcggcgt agtccagccc tcggagatca caagctggcg gcgcctggcc agcagatatc
     3781 cgcgcacggc cagcgcggcg ccggtcagaa gcatggaggg gaggtccagt cctccggtgc
     3841 tggctagtgt gccaaagcca gccagtacaa gcagatacag tgcaacctcg aagtagtggt
     3901 cgatggcgcg ggcgaggggc atcgcgcccg acgccgcgac ctgggccgga gtttgtgcac
     3961 tggcagcaga cataagagcg gacttcttcg tcagatcacc attgttcgac aacagcgaca
     4021 ggaagggaag ggttgagcac gagaatcttg caacgaaagt cacatgtctc agcctttttg
     4081 aaaaccccca cttctcgcaa gaagcgcgag aagtggggca cccgggtctg ggaaaggctg
     4141 ccggctccgg atgctagaat gatcggtcag gcatcggtct cgcgcgacgt actcccgcca
     4201 accccgccag gtccggaagg aagcaacggt aacgggctct tgcgtgtgca gcgagtcccc
     4261 ggtgcctggc tattctccgt tccttctttt tccacgaagc tccggccccg ttaccgattt
     4321 tcggctcacg cgtccaacca ctcgttgtcc gactattcag gaaggctctg atgcaccacg
     4381 accggcacca cggagtcacg gagagattca ttcctaccta ttttctccgt gtctctgtgc
     4441 cgtcgtggtg attaccagtc tcgcctaccg cttttccatg agaaatgccg aaatattctc
     4501 gttcaggaac tttccatccg ctgcgctgcc ggccgcccca gcggtgtgtc cccacaggga
     4561 tggaatgggt ttgagcgtga ccgtggacat gaatccggcc tcgtagcgcg cgtcgccgac
     4621 ggggaaatac aggtcggttt cccctggcat atagaggaac gga
//
LOCUS       DASPUL010000079         4155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_286102, whole genome shotgun sequence.
ACCESSION   DASPUL010000079 DASPUL010000000
VERSION     DASPUL010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286102"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VEJ00_01880"
     CDS             complement(<1..679)
                     /locus_tag="VEJ00_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXW99933.1"
                     /translation="MGFIFLRYIWLIPLLPAFGATVMFFFGRRLQKSTVSAVCVGVVV
                     LAFLLACGCVWQYTNWAAANGHQPYESIGYTWLGSGTGHLTYTTQGGSHAPFNLDARL
                     LLDPLSSIWLLFVTGVGMLIHIYSIGYMGHEGGYYRFFGYLNLFMFSMLTLVLANNYA
                     LMFVGWEGVGLCSYLLIGFYFQRKSASDAANKAFIVNRIGDAGFILGMFFIAWICGSL
                     RFVDVTGF"
     gene            complement(768..1103)
                     /gene="nuoK"
                     /locus_tag="VEJ00_01885"
     CDS             complement(768..1103)
                     /gene="nuoK"
                     /locus_tag="VEJ00_01885"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HXW99934.1"
                     /translation="MQAMLDISTLHYLVVGAALFLLGVIGVLTRRNVVIVLMSIELIL
                     NAVNLNLVALSRYWQGMHGPGSLHGQVFAIFVITDAAAEAAVGLGILIAFFRNKETVN
                     VDEVNLLKW"
     gene            complement(1178..1741)
                     /locus_tag="VEJ00_01890"
     CDS             complement(1178..1741)
                     /locus_tag="VEJ00_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HXW99935.1"
                     /translation="MNPIAPPNPPVATPFFFYLLSGTMILGGILVLTRKNAVHSALAL
                     ITTLLAQAGLYLMLYAPFVAGVQIILYAGGIMVLFLFVIMLINIERNMKERQFNKQWL
                     VGIVAAAALGGLFIAVYTKGTTLFSSARFAYRDPASLYAESGNTQGVAALLFDQHTGM
                     YMFAFEIASLLLLVAIIGAVVMAKKRI"
     gene            complement(1738..3033)
                     /gene="nuoH"
                     /locus_tag="VEJ00_01895"
     CDS             complement(1738..3033)
                     /gene="nuoH"
                     /locus_tag="VEJ00_01895"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HXW99936.1"
                     /translation="MHELINYIESYLGSGQTPGAAGNIFWATVYILLVFVGLSVAVIA
                     MNWLERKILAHMQVRLGPMRVGPHGLLQPIADAIKLLLKEDIIPDGADKLVFWVAPFI
                     VVLAAFTVFVVVPFGPTHAITDMNIGILFMLGVSSLSVLGIVTAGWASNSHYPLIGAL
                     RSSAQMVSYEVAMGLSVVSVIMMTSLSAQGGTLSMIEIVRRQQEQGIWYVFNFFPLGL
                     VGFAIFAIAMVAETNRAPFDLPEAESELTAGFHTEYSGFRWSLFFLAEYSAMIAVSSI
                     AVTLWLGGWLRPFPNWLSGERWDQVFSFFPGFTFLFLAGMTFFNVVRMPKHPFFRIQT
                     IGLAGFGAVLGLIGLILFVPPVRDRVGDIFWFSAKVAAFMYLYIWYRGTFPRYRFDQL
                     MKVGWKVLLPTGLALLILTAGVGMRTELWAAMEDAVHRL"
     gene            complement(3219..>4155)
                     /locus_tag="VEJ00_01900"
     CDS             complement(3219..>4155)
                     /locus_tag="VEJ00_01900"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HXW99937.1"
                     /translation="TYTMFNPYVDRMDYVAAVSNGLGYCEAVEKLLSVEAPPRAQYIR
                     VILTELNRIASHQLWLGTHALDIGAITPLFYTFRDREEILKIFEKYCGARLTTHAFRI
                     GGLQYEAYDGFEKDVKNFIAFVKPKIDEYEELLTTNRIGLERTRGVGVISGKDCIALG
                     VTGPVLRASGVKWDIRKAQPYANYRNFDFEIPTGLNGDTYDRYLVRMVEMRQALRIIE
                     QAVDGIPEGPVMAKVPKVMKPPVGEIYHSIEAPKGELGYFIVSDGSTQPYRVRVRPPS
                     FVNLQALDMMCRGQLVADVIAVIGTLDIVLGEVDR"
BASE COUNT          927 a         1206 c         1270 g          752 t
ORIGIN      
        1 caaatcctgt cacgtcgacg aagcgcagcg acccgcaaat ccaggcgatg aaaaacatgc
       61 cgaggatgaa tccggcgtcg ccgatgcggt tgacgatgaa cgccttattg gctgcatcgc
      121 tggccgactt gcgttgaaaa tagaaaccga tcagcagata cgagcagagc ccgacgcctt
      181 cccagcccac aaacatgagc gcgtagttgt tggcgaggac cagcgtcagc atggagaaca
      241 tgaagaggtt caggtatccg aagaagcggt agtagccgcc ttcatgcccc atgtagccga
      301 tggaataaat atggatcagc atgccgacgc cggtgacaaa cagcagccag atgctggata
      361 gcgggtcgag gagaagtctg gcgtcgagat tgaacggggc gtgggaaccg ccctgggtcg
      421 tatacgtcag atggccagtg ccgctgccga gccaagtgta gccgatactt tcgtagggct
      481 ggtggccatt agccgcagcc caattcgtgt actgccacac gcagccgcag gcgaggagaa
      541 acgccagaac caccacgccc acgcagacgg cactcaccgt cgacttctga agccggcggc
      601 cgaagaagaa catcaccgtg gcgccgaagg ccggcagcag cgggatcagc catatgtatc
      661 ttaaaaagat gaaacccatg tcgttcgtcg tcatcggtac gcggccctga agggccgctc
      721 ttccacggag tacccaggac atctagggca cggctgaagc cgtgccctta ccactttaat
      781 aagttcactt cgtcgacatt gaccgtctcc ttatttctaa agaaagcgat cagaatgcct
      841 agcccaactg cagcttctgc ggcagcatcg gtgattacaa aaatcgcgaa tacctggccg
      901 tgcagtgaac ccggcccgtg cattccctgc cagtagcggg agagcgcgac caggttgaga
      961 ttcacggcgt tcagaatcag ctcgatcgac atcagcacga ttactacgtt ccggcgcgtg
     1021 agcacgccga tcacgcccag caggaacagg gccgcgccaa ccaccagata gtgcagtgtc
     1081 gagatgtcga gcatggcttg catcggctgt tttccggggc taaggccccc cggaatcctg
     1141 aaacctctga tgcggccctg aagggccgct cttccactca aatccttttc ttcgccatca
     1201 cgacggcgcc gatgatcgct accagcagca acagcgacgc gatttcgaag gcgaacatgt
     1261 acatcccggt gtgctggtca aaaagcaagg cggccacgcc ctgcgtattt cctgattctg
     1321 cgtagagcga cgccggatcg cggtaggcga agcgcgcgga ggagaacagc gttgtcccct
     1381 tggtatacac cgcaatgaac aggccgccca gcgccgcggc cgcaacgatc cctaccagcc
     1441 attgtttgtt gaactggcgc tccttcatgt tgcgctcgat gttgatcagc atgatcacaa
     1501 acagaaacag caccatgatg ccaccggcat agagaatgat ttgcacgccg gcgacgaacg
     1561 gggcgtagag catcaggtaa agcccggcct gggcgagcaa ggtcgtgatc agggcaaggg
     1621 cagaatgcac ggcattcttg cgcgtgagca ccaggatccc gccgaggatc atcgtcccgg
     1681 agagcaggta aaagaagaac ggcgttgcga ctggcggatt tggtggcgct atcggattca
     1741 taggcggtgc accgcgtctt ccatcgctgc ccagagttca gttcgcatgc ccactccggc
     1801 ggtcaggatc aacagcgcca gccccgtggg caggagcacc ttccacccta ccttcatgag
     1861 ctggtcaaag cggtaccgcg ggaacgtgcc acgataccag atatagaggt acatgaacgc
     1921 ggcgactttg gccgagaacc agaaaatgtc gcccacgcgg tcgcgcaccg gcggaacgaa
     1981 cagaatcaga ccgatcaggc cgagcacggc gccaaaaccg gccaggccga tggtttgaat
     2041 ccggaagaaa ggatgcttgg gcatgcgaac gacgttgaag aacgtcatgc ccgccagaaa
     2101 caaaaacgtg aacccaggaa agaacgagaa gacttggtcc cagcgctcgc cgctcagcca
     2161 gttggggaac ggccgcagcc agccgccgag ccacagcgtc accgcgatcg aggagacggc
     2221 gatcatggcc gagtattcgg caaggaagaa cagggaccag cggaatccgc tgtactcggt
     2281 atggaagccg gcggtcagtt cggactcagc ttcgggcaga tcgaagggcg cgcggttggt
     2341 ctcggcgacc atcgcgattg caaagatggc aaagcccaca aggccgagag gaaagaaatt
     2401 gaagacgtac cagattccct gctcctgctg gcgcctgaca atttcaatca tgctcagcgt
     2461 gccgccctgc gcgctgaggc tggtcatcat gatgaccgaa accacggaga ggcccatagc
     2521 gacttcatag gaaaccatct gggcgctgga gcggagcgcg ccgatcagcg ggtagtgcga
     2581 gttcgaagcc catcccgcgg tgacgatacc gagcacgctg agcgacgaca cgcccagcat
     2641 aaagaggatg ccgatgttca tgtcggtgat ggcgtgcgtg ggaccgaaag gcacgaccac
     2701 gaagacggtg aacgcggcga gcacgacgat gaacggcgcc acccagaaaa ccagcttgtc
     2761 ggcgccgtcg ggaataatgt cttccttgag cagcagcttg atggcatcgg cgatgggctg
     2821 cagcagaccg tgcggaccca cgcgcatggg accgaggcgc acctgcatat gcgcgagaat
     2881 tttgcgctcc agccagttca tcgcgatgac cgcgacggac aggcctacga agaccaggag
     2941 gatgtagacc gtagcccaga agatgtttcc ggcggcgccc ggcgtctggc cgcttccgag
     3001 ataggactcg atgtaattaa tcagctcgtg catttagcgt gatctggggc taaagccccg
     3061 gttcttctgt attcctcgca cgcggcccta aagggccgct cttccacgat ggttcatgcg
     3121 tcggtgttgg ctcgttggct ccttgcggct aaagccgcaa atcctttgcg actctttcgg
     3181 cgcggcttga agccgcgccc tttcaaaaca gatcctggtc agcgatccac ctctcccaga
     3241 acgatgtcga gggtgccgat taccgcgatg acgtccgcaa ccagctggcc gcggcacatc
     3301 atgtcgagag cttgcaagtt cacgaaactc ggcgggcgga cacgcacgcg atagggctgc
     3361 gtcgagccgt cactcacgat gaagtatccc agttcgcctt tgggcgcttc gatcgagtgg
     3421 tagatttcgc cgacgggagg tttcatcacc ttgggaactt tagccatgac cgggccttcc
     3481 gggatgccgt cgaccgcctg ctcgatgatg cgcagcgcct gccgcatttc gaccatgcgc
     3541 acgagatacc ggtcgtatgt gtcgccgttc aggcccgtcg gaatttcgaa atcgaaattt
     3601 ctgtagttgg catagggctg ggctttgcgg atgtcccact tcacgccgct ggcgcggagc
     3661 acgggaccgg tcaccccgag ggcgatgcaa tccttgcccg agatgacgcc aacccccctg
     3721 gttcgttcga gcccgatgcg gttggtggta agcagctctt cgtattcgtc gatcttcggc
     3781 ttcacgaagg cgatgaagtt cttgacgtct ttctcgaagc cgtcgtaggc ctcgtactgc
     3841 aggccgccga tacggaaggc gtgggtggtg aggcgcgcgc cgcagtattt ctcgaagatc
     3901 ttgagaattt cttcgcggtc gcggaatgtg tagaagagcg gcgtgatcgc gccgatgtcg
     3961 agagcgtgcg tgcccagcca cagctgatgg ctggcgatgc ggttcagttc ggtcaggatg
     4021 acgcgaatgt actgggcacg gggcggggct tccacgctga gaagtttttc gaccgcctcg
     4081 cagtatccca ggccgttcga gaccgcggcg acgtagtcca tgcggtcgac gtagggattg
     4141 aacatcgtgt aggtg
//
LOCUS       DASPUL010000080         4312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_834730, whole genome shotgun sequence.
ACCESSION   DASPUL010000080 DASPUL010000000
VERSION     DASPUL010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834730"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="VEJ00_01905"
     CDS             <1..1321
                     /locus_tag="VEJ00_01905"
                     /inference="COORDINATES: protein motif:HMM:NF021504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HXW99938.1"
                     /translation="KEVFRPTNAKSVADPLPTKGWAGWRKGDKERCALMENRKKVRIG
                     IPRVLNIYTYAPLFNAYFESVGVQPENIIYSDYTSSELYRAGASRGAIDPCFPAKIGI
                     SHVYNLIQEKHRKKPLNAIFFPMYDVLHSPLVKIVGANACPTVTATPETVKAAFTKEN
                     DVFAEHNVKYIDPVLNFTDRKLFAHQMLQAWQPILGLSLEENDRAVESGFQALKEYET
                     NIRRRARQVLDQLEREDRIGIVMLGRPYHHDPGLNHEILDEFQKLGYPIFSQNTLPLD
                     EDLLERLFGEEVRAGVVSSPLDITDAWKNSYSCSTNHKVWAAKFTARHPNLVALEISS
                     FKCGHDAPIYGVIEGIIEQSGTPYFCFKDLDENKPSGSIRIRVETIDYFLRRYREEVI
                     RKRKAEQEIENQLALLEMQLRGGNMQDEIAPPGIEQLEPEAQGMAAD"
     gene            complement(1368..1823)
                     /locus_tag="VEJ00_01910"
     CDS             complement(1368..1823)
                     /locus_tag="VEJ00_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXW99939.1"
                     /translation="MPRTAKVSQDRLTFNHAMVYSRDVQRALGFYRDLLGFKLVDEFR
                     HENRPVYARLRAPGGDGTLALHLADAGTPIVSDGVRLYFEIRELEDFCRKLQAKGFYI
                     TQMPRMMPWGWLHAYLNDPDGHEISLYWAGENRMKKTVMRTAKQIEERK"
     gene            1996..2766
                     /locus_tag="VEJ00_01915"
     CDS             1996..2766
                     /locus_tag="VEJ00_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXW99940.1"
                     /translation="MIATIDHGDVRELRLNRPPVNALSPELITALLDAVRTAPHEGKR
                     ALLLSGLPGMFSAGLDVPRLLKLDRPAMDALWRDFYALFSALACSPIPIAAAITGHAP
                     AGGTVLALFCDHRVAAEGDWRMGLSEVQVGLPLPPVILTALRRLVGARHAERLAVRGL
                     LITTAEAAVCGLVDELAPADQVVDRGLKWCETLLALPRTAMEITRQQARADLVAAFAP
                     QTAQELAEVTRWWWSEETQKALHRMVEQLAMKKKSARD"
     gene            3002..>4312
                     /locus_tag="VEJ00_01920"
     CDS             3002..>4312
                     /locus_tag="VEJ00_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99941.1"
                     /translation="MEKTRDQQSRAESKSRLLEQINILLHSGDYSRALDLLQDAAREL
                     PHDAELSELEKRAQDGVKRNAEAQRLITESQELFAQQKSTEAIQLLREAYELDKTNAL
                     ARAILANALVEHAHSILETDWLGAETLVNQAVALNPAHPTAKTILNAIVEQKETSSVE
                     DWVSQARKLQSSGDLMAALAWVAEGLAVHPHNPRLLQIQDEIQRDQGARRRQARRSDL
                     EDLRRMELEIDKAPDVAAKQALAERIQAMAARYWTDGEILSIANGLLQRLGLVPESST
                     ASPHGAGAPVIFHVPRPGPPKPASADTRQIPAARIPPAEVTPSSVPSGNIPGGDVPAA
                     AIPPTTVPPSTLPDQVLTSRQEPQFPAPQSASVSVLEPSAPAAGTSPSQPKQPTRSNS
                     ITLVLVCSSAVTIILLAAIFFFARRHHTPPVGKTPVLAPTVSGPA"
BASE COUNT          908 a         1330 c         1220 g          854 t
ORIGIN      
        1 aaaagaagtc ttccgcccca cgaacgcgaa gagcgtagct gatcccctgc cgacgaaagg
       61 atgggccggc tggagaaagg gcgacaaaga gcgctgcgcg ctgatggaga accgcaagaa
      121 ggtccgcatc ggcattccgc gcgtgctgaa catctatacc tacgctccgc tgttcaacgc
      181 gtattttgaa agcgtgggcg tgcagccgga aaacatcatt tattcggatt acacaagctc
      241 ggaactttat cgagctggag ccagccgggg cgccatcgac ccctgcttcc cggcgaagat
      301 cggtatctcg cacgtgtaca atctgattca agagaaacac cgcaagaagc cgctaaacgc
      361 gattttcttc cccatgtacg acgtgctgca ttcgcccctt gtgaaaattg ttggcgcgaa
      421 tgcatgtccg acggtgaccg caactcccga aaccgtgaag gcagcattta ccaaagagaa
      481 cgacgtcttc gccgagcaca atgtgaagta catcgacccg gtgctcaact ttacagaccg
      541 gaagctgttc gctcaccaga tgctgcaagc ctggcagcca attctgggct tgtcgctgga
      601 ggaaaacgat cgcgccgtcg aatctggatt tcaggcattg aaagaatacg agacaaacat
      661 tcgccggcgt gcgcggcagg tgctcgatca actggagcgc gaagaccgca tcggtatcgt
      721 catgctgggg cggccgtacc atcatgatcc cgggctcaac cacgagattc tcgatgagtt
      781 ccagaagctc ggctatccga tcttttctca gaacacgctt ccgctcgatg aggaccttct
      841 ggagcggctt tttggcgaag aagtgcgcgc cggagtcgtc agcagcccgc tcgacatcac
      901 cgacgcctgg aagaactcgt actcctgcag caccaaccat aaggtctggg cggcgaagtt
      961 tacggcgcgg catcccaacc tggtggcgct cgaaatttcg agcttcaaat gcggtcacga
     1021 tgcgcccatt tatggcgtga tcgaaggaat catcgagcag tcgggcacac catacttctg
     1081 cttcaaggat ctcgacgaga acaagccgtc cggctcgatc cggattcgcg tggaaaccat
     1141 cgactatttc ttgcgtcgct accgcgagga ggtcattcgc aagcgcaagg ccgagcagga
     1201 gatcgaaaat caactcgcat tgcttgaaat gcagttgcgt ggcgggaata tgcaggatga
     1261 aatagccccg ccagggatcg agcagctgga accggaagcg cagggaatgg ctgccgacta
     1321 gaaaaagcga ggaatcatcc cgaaccggcc agcactgaat cttgcgtcta ctttcgttct
     1381 tcgatctgtt tggccgttcg catgaccgtt ttcttcatgc ggttctcgcc tgcccaataa
     1441 aggctgatct cgtggccatc ggggtcattc agataggcgt gtagccagcc ccacggcatc
     1501 atgcgcggca tttgcgtgat gtaaaagcct ttcgcttgca gcttgcggca aaagtcttcg
     1561 agctcgcgaa tttcgaagta tagccgtact ccatcgctga cgataggagt cccggcgtct
     1621 gccaggtgca atgcgagagt gccatcgccg ccgggtgcgc gcaatcgcgc atacactggc
     1681 ctgttctcgt ggcggaactc gtccaccagc ttgaatccga gcagatcgcg ataaaaaccg
     1741 agggcgcgct gcacatcccg cgagtacacc atcgcatggt tgaaggtcag tctatcctga
     1801 ctgaccttcg ctgttcgagg catgcttttc tttgactggg cggtattgcg cggcttcttt
     1861 ggggtcgatt tccgagcttg acgtgcctgg ggtgtctttt tcttcttcgg actgactctc
     1921 gccatggcgc cctcgcaatt gccttgacat tctatacttg ctgcgtacag gtcgcgttcc
     1981 tgatatttat ccaacatgat tgcaaccatc gaccacgggg acgtccggga actcaggctg
     2041 aaccggccgc ccgtaaatgc gctctctcca gaactgatta ctgcccttct ggacgctgta
     2101 cgaaccgccc cgcacgaagg caagcgagcc ctcctgctct cgggcttgcc gggaatgttc
     2161 tcggccggac tggacgtgcc tcggctcctt aagctcgacc ggccggcgat ggatgccctt
     2221 tggcgtgact tttatgcgct cttcagcgcg cttgcttgct cccctattcc cattgcggcc
     2281 gcaatcaccg gccacgcgcc cgctggtgga accgtgctcg cgctcttttg cgaccaccgg
     2341 gtcgcggctg agggggactg gagaatgggc ctcagcgaag tgcaggtcgg attgccgctg
     2401 ccaccggtga tcctcaccgc cttacgccgc ctcgtcggcg cgcgccatgc cgagcgcctg
     2461 gctgtgcgcg ggctcctcat aaccacagcc gaagccgcgg tctgcgggct tgtggacgaa
     2521 ctggctcctg cggaccaggt tgtagaccgc gggctaaagt ggtgcgagac tttgcttgcg
     2581 cttccccgga ccgctatgga gatcactcgc cagcaagccc gcgcagatct cgtcgcggcg
     2641 tttgcgccac aaaccgccca ggaactggcc gaggtcacgc gctggtggtg gagcgaggag
     2701 acgcaaaagg cgctccatcg gatggtggag caactcgcca tgaagaagaa atcggcgcgc
     2761 gattagaccc atgcataaat ggacatgccc cgcgacgcaa ctacccggac actagccttt
     2821 tccccccaat tgagtaagat ttttgaagcc acgcagaacg gggtgggcga tgaagatccc
     2881 cgcttcacca gagaattgac atgcgatgac gaggtcccgc aaccttggcc aaaacatgcg
     2941 cccgctctgt tgcgccgacg aaacggtcgt gcgccatgtc tgaccttcga ggttagagcg
     3001 cttggagaaa acacgcgacc agcaaagccg cgccgaatcc aaatcgcggc tgcttgagca
     3061 aatcaacatt cttctgcatt cgggcgatta ctcacgggct ctggatttgt tgcaagacgc
     3121 ggcccgcgaa ctcccccatg atgcggagct gtccgaactg gaaaagcgtg cccaggatgg
     3181 cgtcaagcgc aatgccgaag cccagcgtct tatcaccgaa agccaggaac tgttcgcaca
     3241 gcagaaatcc accgaagcga ttcagttgct gcgcgaagcg tatgagctgg ataagaccaa
     3301 tgctctcgcc cgcgccattt tggcgaatgc cctggtcgag catgcacatt ccattctaga
     3361 aaccgattgg ctgggagccg agacattggt caatcaggcg gtcgccttga accctgcgca
     3421 tcccacggcc aaaacgatcc tcaacgcgat cgtggagcag aaggaaacga gctcggtcga
     3481 ggactgggtt tcgcaagcac gcaagctgca gtcctctggc gatctaatgg cagccttggc
     3541 ttgggttgca gaaggattgg ccgtccatcc tcacaatccc aggttgcttc agattcagga
     3601 tgaaatccag cgcgaccagg gtgctcggcg acgccaggct cgccgtagtg atttggaaga
     3661 cctgcggcgc atggagcttg aaatcgataa ggccccggac gttgctgcga aacaagcact
     3721 agcggagcgc atacaagcaa tggccgccag gtattggacg gatggagaaa tcctgtccat
     3781 cgccaacggc cttctacagc ggctgggatt ggtccccgag agttcaaccg cgtcgccgca
     3841 cggcgcgggc gcccccgtca tttttcacgt cccgcgccct ggtcctccga agcctgcatc
     3901 ggccgatacc aggcagattc cggcagcccg gattccgcca gccgaggtta cgccaagctc
     3961 ggttccatcc ggaaacatcc cgggcggaga tgtcccggcc gccgctattc ccccgaccac
     4021 ggttccgccc agcaccctgc cggaccaagt gctgacttct cggcaagagc cgcagttccc
     4081 cgcaccgcaa tccgcctccg tttcagtcct cgagccctct gcgccagcgg ccggaacgtc
     4141 cccatcgcag ccaaaacagc ctacgaggtc gaactcgatc acgctggtac tcgtctgctc
     4201 ctccgccgtc acgataatcc tgcttgcagc gatcttcttc tttgcccgaa ggcaccacac
     4261 cccgcccgta ggcaagactc ccgtactcgc tcccacggtc tcgggacccg cc
//
LOCUS       DASPUL010000081         2081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1793390, whole genome shotgun sequence.
ACCESSION   DASPUL010000081 DASPUL010000000
VERSION     DASPUL010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793390"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1515)
                     /locus_tag="VEJ00_01925"
     CDS             complement(214..1515)
                     /locus_tag="VEJ00_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99942.1"
                     /translation="MQRGQLRGARSLLAPLLILTAASSLQAQQPRFETGSRTQQRVLA
                     DAMRQNSYPLSLQDGVLRGPGFDFLARRSANAQFVVVGEEHFVKEIPEFLSALFAFLH
                     EKHGFNYLALENDPVSAHLASLPPLRGDLQALGRYAQQYPNAFTFPTDQELRLIADAG
                     RWSQGKIDPVWGLDQSFGVLHALDRLQALPGFRDTPMFQQLHQEALAADTRRPPSGDH
                     PDQLDYMQSVKLADLEELRRQIDAPEGSEARFILDNLVASSQIYGYYLRSEAGELTRY
                     ANGFEREQMMKRLFMRQYHAAEAHGEHNPKVLLKFGGWHVFRGLGPSQLQTLGNFVTE
                     LATANGTDGLTVGVYLRGSWRDIGKQKGMEPIAAATDPSSWTVVDFAALRPAIRAEKF
                     GALDPTLLSHISGFDVALVIGGASEATESLRSQPKPSPPKP"
     gene            1704..>2081
                     /locus_tag="VEJ00_01930"
     CDS             1704..>2081
                     /locus_tag="VEJ00_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXW99943.1"
                     /translation="MKLGEKIRYLREVEGSLRGLGRPMTQLELVRAIRQELRGAGKGA
                     EKTTISQSYLSQIENGARPHMTQSSRALLAKFFKVHPGFLVDDPDGYHAELTSDLRTT
                     EGQLDVWLLQGSERFASDPEVSNV"
BASE COUNT          437 a          607 c          655 g          382 t
ORIGIN      
        1 gagaaggcgc aggtcaaccg gcagcagaac aagatgtcgc gccagatcta ccgcgacaaa
       61 cacaacggcc gccatcaata agttgcattc agaagcaccg gatgcccgcg gctcaaaccg
      121 cgggccttct tattggagag agacttctta cgaacaactg aacttaaggc ggcgcgatgg
      181 gcaggagtgc cgcagcgatc gggctatggc gcttcacggt ttaggcggcg aaggtttggg
      241 ctgcgagcgc agcgattcgg ttgcctcgga ggctcctcca atcaccagtg cgacgtcgaa
      301 gccggaaatg tgactgagga gagtcgggtc cagggctcca aacttctccg cccgaatcgc
      361 cggccgcagg gcggcaaaat cgacaaccgt ccacgacgac gggtccgtgg ccgcagcgat
      421 cggctccatc cccttttgct tgccaatatc tcgccaggag ccgcgcaagt acacgcctac
      481 cgtgagcccg tccgtcccgt tggcggtcgc gagttcggtg acgaaatttc ccagggtttg
      541 cagttggctt gggcccagtc cgcggaaaac gtgccagccc ccgaacttca aaagcacctt
      601 cgggttgtgc tcgccgtgag cttcggcagc gtgatattgc cgcataaaaa ggcgcttcat
      661 catctgttcg cgctcgaatc cgtttgcgta acgggtaagc tctccagctt ctgagcggag
      721 atagtagccg tagatctggg acgacgcgac aagattatcc aggatgaatc gggcctccga
      781 tccctcggga gcgtcaatct ggcggcgcag ctcttcgagg tcggcaagct tgacgctctg
      841 catgtaatcc aactgatcgg ggtgatcgcc gctcgggggg cgtctcgtat ccgctgccag
      901 cgcttcctga tggagttgct gaaacatggg cgtatcgcgg aatccgggaa gagcctgcag
      961 ccgatcgagc gcatgcagga cgccaaaaga ctgatccagc ccccagaccg gatcgatttt
     1021 tccctggctc cagcgcccgg cgtcggcgat caggcgtagc tcctgatcgg taggaaacgt
     1081 gaacgcgttt ggatattgct gagcgtagcg accgagggcc tgcaaatcac cccgcagcgg
     1141 cggcagggaa gcaaggtgag cggagacggg atcgttttcc agggcaaggt agttgaatcc
     1201 gtgcttttcg tgcaggaagg caaacaacgc gctcaggaac tccgggatct ctttgacgaa
     1261 gtgctcttcg cccaccacaa cgaactgcgc gttggccgag cgccgcgcca gaaagtcgaa
     1321 tcccggcccg cgcagcacgc catcctgcag ggagagaggg tagctgttct gccgcatcgc
     1381 atcggccaga actcgttgtt gtgtgcggga accggtttcg aatcgcggct gctgcgcctg
     1441 cagggaagac gctgccgtca ggatgagcag tggcgcgagc agcgaacgcg caccccgcaa
     1501 ctggcctcgt tgcattgccg ttcccccgta gtgtgcgcta aaggcactcg tgccaccagg
     1561 gctttagcga atgccggctg accccaaggg ctgagcgcaa ctggttgaga atacgccaga
     1621 tcctttcctt gacaataatt gcatattgca atataaatat ctcgatttga aatactaaat
     1681 acagcgacag tggggcgtgg ccggtgaagc tgggcgagaa aatccgctac ctgcgggaag
     1741 tcgaaggatc gctgcgcggg ctgggaaggc cgatgacgca gttggaactg gtgcgggcca
     1801 tccgccagga gctgcgcggc gccggcaagg gcgcggaaaa aacgaccatc agccagtcgt
     1861 acctgtcgca gatcgagaac ggggcgcggc cccacatgac gcagtcctcg cgggcactgc
     1921 tggccaagtt cttcaaggtg catcccgggt ttctggtgga tgatcccgac ggctatcacg
     1981 ctgaactgac ttccgatctg cgcacaaccg agggccagct cgacgtctgg ctgttgcagg
     2041 gctcggaacg atttgccagc gatccggaag tcagcaacgt g
//
LOCUS       DASPUL010000082         5064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1246026, whole genome shotgun sequence.
ACCESSION   DASPUL010000082 DASPUL010000000
VERSION     DASPUL010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246026"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VEJ00_01935"
     CDS             complement(<1..261)
                     /locus_tag="VEJ00_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99944.1"
                     /translation="MFSDRTNWRLAQNRLTLALQEARARGSKILDLTVSNPTRIGLEY
                     DSQAILAALGSARGLDYDPQAKGLPAAREAVAAYYRRDHGVHD"
     gene            complement(436..1011)
                     /locus_tag="VEJ00_01940"
     CDS             complement(436..1011)
                     /locus_tag="VEJ00_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HXW99945.1"
                     /translation="MRKLNVILFISVLCALVLPGRAVPDQTSQPEQQLKLVPAPQEVR
                     LYGPGGFRVGARTKILVQLGHQEEDRIAAETLAETVSDQSGLQLDILGMKAAGKAEGG
                     AIVLARLQDARVRRFLARKGLRADELVGEQGYLLFSDKSHLIVAANSGQGLFSGVQTL
                     RQLLRPTRKELLCPAVTIRDWPRLTESAMKN"
     gene            1226..2581
                     /locus_tag="VEJ00_01945"
     CDS             1226..2581
                     /locus_tag="VEJ00_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXW99946.1"
                     /translation="MEPFRFHLFVCTQQKPEGVPSCPASGSFAVLEALDHAIQERNLD
                     QDLQLTTCGCMGLCDEGPMMVVYPGGVWYRRVRPADVPEIVDTHMHAGKPVDRLVWND
                     APAMQAMAVEHTEKFRAAMAAREKAGTLPDRLDQMSRGYMPSRCLLTALELDIFTAVG
                     AGANAERIGAKINANPRAAGLLLNALVSLGLLSKTGDEYRNTPESARFFTQGSKDNHR
                     NGLLHIANIWHRWSTLTDAVRAGTRIPIDRDRTPEWTRSFIAAMQRNAGERAPLVVKA
                     LGTAGVRRILDLGGGSGAYSIAFAKASPDLQCEILDLPEVVPLAADYVSKAGVSAQVK
                     LRPGDMLHDNFGSGYDLVMLNAICHMFSEEQNQDIFRRARQALAPHGRLAVQDFILNP
                     DKTGPQQAALFSLNMLVSTDSGASYSEAEYSRWMKAAGFSEVQRINLPGPSALILGRA
                     S"
     gene            2646..5063
                     /locus_tag="VEJ00_01950"
     CDS             2646..5063
                     /locus_tag="VEJ00_01950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXW99947.1"
                     /translation="MQAFSRNLRFGFRLLTRNPGFTAVTLLTLALGIGANTAIFTVTS
                     ALLLRPFPYRDPQQLVGVTPRDSNGDRPSTLLRYELVCGANQSFQSVAVWANDNLNLT
                     GSGEPIQVPIARVSPSFFSMLGIRPQLGRIFTEDEGRPEGKPVVMLTDSIWRSRFHAD
                     PNIVNQPLTLDGTPQTIVGVLPRDVQFPFVGEADIWTPRYFEFSLMSPQRLRMGVGYL
                     NIMARLRPGTTLAQANAELALLNQRYREQNPSAPDSDPAMKMLSDPLRDVVVGDVRGK
                     VLMLSAAVGLVLLIACANVASLLLSRALARKREIAVRTALGASRATLVWQLLTESMML
                     ALVAGILGIGLSWMATRALLAWGASQLPPGIPIGMDYRVLLFTLVISIFAGIIFGTIP
                     ALQLARSDPNSSLRDQGRGLSVSRSHAQMKNLLVVSQVALSLLLVIGSGLLLRSFVRL
                     LRVDPGFDARNVLTMDISLPTVKYSKPDQQIAFFDEVLRRVSALPGVRNAATSATLPL
                     HFIRIAPVLPEGQPDVPLSQRPFVDVEAVSPQWFQTLRVPLRGGRDFTAADNAQAPKV
                     VVINETFARQFWPNDNPIGKHMIVGRWTTPAEVVGVSADIKNNGLEPDTKPQIYLPFP
                     QLPWGNMNLLIRTALPPRSITSAVRAQIAAVDPDQPVMKIQTVDDLMDASRSEPRFTM
                     LLLSVFSATALVLAVIGIYGVLSYSVAQRRQEFGIRLALGAEHADILRLVVRHGLMLA
                     AAGIVLGLIAALFLTRLMSSMLYKVGARDLTTFVLTPLLFASIAALASYLPARRATKV
                     SPIEALR"
BASE COUNT          918 a         1710 c         1417 g         1019 t
ORIGIN      
        1 atcatgaaca ccatggtccc gccgatagta ggccgccacc gcctcccgcg ccgccggtaa
       61 tcctttcgcc tgcggatcgt aatccagccc ccgtgctgaa ccgagtgccg ctaggatcgc
      121 ctgcgagtca tactccaacc cgatccgcgt cggattcgat acggtcaagt cgagaatctt
      181 cgagccccgg gcgcgcgcct cctgtaatgc gagggtcaaa cggttctgag ccagcctcca
      241 gttggtgcga tcagaaaaca taccttcgat atccgggatt ctatgggatg gtgggtactt
      301 ctgacaggca ggaacgaaag aaaaagaata agtgcgtgaa cggtctcgac gctgacttcg
      361 cgctcaacgc gtctcacgct cctacaaaca agccgtgcca aaaggacggc tgatctcttc
      421 cttctacgcc gctgttcaat tcttcattgc gctttcggta agccggggcc agtcccgaat
      481 cgttaccgct ggacagagaa gctccttcct tgtcggacgc agcaactgcc gcagggtctg
      541 cacaccggaa aacagtccct gcccgctgtt ggcggccaca atcaggtgcg atttgtccga
      601 gaacaataga tagccctgct cgcctacgag ttcatccgct cgcaagccct ttctcgccag
      661 gaatcgccgc acgcgagcgt cttgtagccg cgctaacaca atcgcgccgc cttccgcctt
      721 ccccgcggcc ttcatgccca gaatgtcgag ctgcaggccc gattgatcgc tgacggtttc
      781 ggccaaggtc tcggcggcaa tccggtcttc ttcctggtgc ccgagctgca caaggatctt
      841 cgtccgcgcc cccacgcgaa aaccgccggg gccgtagagc cgcacttcct gcggcgcggg
      901 caccagcttc agttgttgct cgggttgcga cgtctggtcc ggcaccgccc ggcccggaag
      961 aaccagcgca cacaagacgg agatgaacag aataacgttg agttttctca ttcagtcctg
     1021 aaaaaccttc caacgaaccc cactattgta ccggtgtgtg aagggatctg tgtctcaact
     1081 ttgtcaacat cccgataact ccaaccaaaa tcatggttta tctacccccg ctgttcgccc
     1141 ttcccgcccc gcaaagcgct aacattgacg tgtagacctg atccgaggag caattctaca
     1201 aaactcatat cgaccggaga ctctcatgga accattccgc tttcatcttt tcgtttgcac
     1261 acagcaaaaa cccgaaggcg taccctcttg cccggccagc ggatcattcg ctgtgctcga
     1321 agcgctcgat catgccatac aggaacgcaa cctcgaccag gacttgcagc tcactacctg
     1381 cggctgtatg gggctgtgcg atgaaggccc catgatggtc gtgtatccgg gaggagtgtg
     1441 gtaccggcgc gtgcggccgg ccgatgtgcc tgaaatcgtc gacacgcaca tgcacgccgg
     1501 caagcctgtc gatcgcctcg tgtggaacga cgccccggca atgcaagcca tggccgtcga
     1561 gcacactgaa aagttccgcg cggcgatggc tgcgcgcgag aaagccggca cattgccgga
     1621 ccgcctcgat cagatgagtc gcggctacat gcccagtcgt tgtcttctca ctgcgctcga
     1681 actcgacatc ttcaccgccg taggtgctgg agccaacgcc gagcggatcg gagcaaagat
     1741 caacgcgaac ccgcgtgcgg ctggcctgtt gctcaatgct ctggtctccc tcggactgct
     1801 ctcaaaaact ggcgacgagt acaggaacac gcccgagtca gcccgcttct tcacccaggg
     1861 gtccaaagac aatcatcgca acggcctgct gcacatcgcc aacatctggc atcgctggag
     1921 caccctgacc gacgccgtgc gtgccggcac gcgcattccg attgatcgtg accgcacgcc
     1981 tgagtggaca cgcagcttta tagctgccat gcaacgaaac gccggggaac gcgccccgct
     2041 tgtggtaaag gccctcggta cggcgggtgt tcgccgcatt cttgatctgg gcggtgggtc
     2101 gggagcctac tctatcgcct ttgcgaaggc gtctccggac ctgcaatgcg aaattctgga
     2161 cctgcctgag gttgtgccgc tcgccgcgga ctatgtcagc aaagctggag tctcagccca
     2221 agtcaagctt cgacccggcg acatgctcca tgacaacttc ggctctggct acgaccttgt
     2281 catgctgaac gcgatctgcc acatgttttc cgaggagcaa aatcaggaca tcttccgccg
     2341 cgcccgccag gctctcgcgc cccacggccg cctggccgtg caggatttca tcctcaatcc
     2401 tgacaaaaca gggcctcaac aagcggcgtt gttctcgctc aacatgcttg tctccaccga
     2461 ctctggtgcc agctacagcg aagccgaata ctcgcgctgg atgaaggccg cggggttcag
     2521 tgaggtgcag cggatcaacc tgcccggccc gagcgctctg attctaggtc gagcgagcta
     2581 gcgcggattt ttggatccag cgcggggcac agtttgacct gttccgccgc tataatttca
     2641 caaccatgca agctttttcg cgcaacctgc gcttcggctt ccgcctgctc accaggaatc
     2701 ccggattcac cgccgtcacg ctgctcacgc tggctttggg aatcggcgcg aacaccgcca
     2761 tcttcacggt gaccagcgcg ctgcttctgc gcccatttcc ctatcgcgac ccgcagcaac
     2821 ttgtgggcgt cacgccgcgc gacagcaacg gagaccgccc cagcacgctc ctgcgctacg
     2881 aattggtgtg cggcgccaat cagtcgtttc aatcggtggc cgtatgggcg aatgacaatc
     2941 tcaatctcac aggttcgggc gaaccgattc aagtgccgat cgcgcgcgtc tcgcccagct
     3001 ttttctcgat gctcgggata aggcctcagt tgggccgaat cttcaccgaa gacgaaggcc
     3061 gccccgaagg caagccggtg gtcatgctca ccgattcgat ctggcgcagc cgcttccatg
     3121 ccgatccgaa catcgtcaac cagcccctga cgctcgacgg caccccgcag accatcgtcg
     3181 gagtgctccc gcgcgacgtg cagtttcctt tcgtcggcga agctgacatc tggacgccgc
     3241 gttacttcga gttctctttg atgagtccgc agcgcctgcg catgggcgtg ggatatctca
     3301 atatcatggc gcgcctgcgc ccgggaacca cgctggcgca agccaatgcc gagcttgccc
     3361 tgctcaacca gcgctatcgc gagcagaatc cgagcgcgcc tgactccgat cccgccatga
     3421 agatgctttc cgacccgctg cgcgatgttg tagtgggcga cgtccgtggg aaggtgctca
     3481 tgctctcggc cgccgtcggc cttgtcctgc tgatagcctg cgccaacgtg gccagcctgc
     3541 tgctctcccg cgccctcgct cgcaaacggg agattgccgt acgcaccgca ctcggagcca
     3601 gccgcgccac gctggtctgg cagcttctga ccgagagcat gatgctggcg ctcgtggccg
     3661 gcatccttgg catcgggctg agctggatgg cgacccgcgc cctccttgcc tggggtgcaa
     3721 gccagcttcc gccgggcatc cccatcggca tggactaccg cgtgcttctg ttcacgctgg
     3781 tgatctccat cttcgccgga atcatcttcg ggactattcc cgcgctgcaa ctcgcccgct
     3841 ccgatccgaa ctcttccctg cgcgatcagg ggcgcgggct gtcggttagc cgctctcacg
     3901 cgcagatgaa gaacctcctt gtcgtcagcc aggtggcgct ttcgctgctt cttgtcatcg
     3961 gctccggact cctgttgcgc agtttcgtgc gcttgctccg cgttgatccc ggctttgacg
     4021 cccgcaacgt gctcaccatg gacatctcgc tgccaacggt gaagtactcc aagcccgacc
     4081 agcaaatcgc cttttttgac gaagtcctgc gccgcgtctc cgccctgccc ggcgtgcgaa
     4141 acgctgcgac ttcggcaacc ctgccgttgc acttcatccg cattgccccg gtgctgcccg
     4201 agggtcagcc ggacgtccct ctctcccagc gtccgttcgt cgacgtggag gctgtgagcc
     4261 cgcagtggtt ccagaccctg cgcgtcccct tgcgcggcgg ccgcgacttt accgccgccg
     4321 acaacgccca ggcgccaaag gttgtggtca tcaatgagac gttcgcccgc cagttctggc
     4381 ccaatgacaa tcccatcggc aagcacatga tcgtcggccg ctggaccacg cccgcggagg
     4441 tggttggcgt atctgccgac atcaagaaca acggcctcga gcccgacacg aagccgcaga
     4501 tctatcttcc ctttccgcag ttgccctggg gaaacatgaa cctgctgatt cgcacggccc
     4561 tcccgccgcg cagcatcacg tcagccgtgc gcgcgcaaat cgcagcggtc gatcccgacc
     4621 agcccgtcat gaagatccag actgtcgacg atctgatgga tgcttcacgc tccgagccgc
     4681 gcttcaccat gctgctgctg agcgtgttct ctgcgaccgc cctggtcctg gcggtaatag
     4741 ggatttatgg ggtgttgtct tattccgtgg cgcagcgccg ccaggagttc ggcatccgtc
     4801 tcgcgctcgg cgccgagcat gctgacatcc tgcggctcgt cgtccgccac ggactcatgc
     4861 tggctgcggc gggcatcgtg cttggcctga tcgcggcgct cttcctgacg cggctgatgt
     4921 cgagcatgtt gtacaaagtc ggggcgcgcg acctgacgac gttcgtgctc acgccgctgc
     4981 tcttcgcctc aattgcggcc ctggccagct atctcccggc gcgccgggcg acgaaggtca
     5041 gtcccatcga agcgcttcgt tgaa
//
LOCUS       DASPUL010000083         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_972241, whole genome shotgun sequence.
ACCESSION   DASPUL010000083 DASPUL010000000
VERSION     DASPUL010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972241"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /locus_tag="VEJ00_01955"
     CDS             <1..1785
                     /locus_tag="VEJ00_01955"
                     /inference="COORDINATES: protein motif:HMM:NF019880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HXW99948.1"
                     /translation="PSFSVHATRQFYHCFGCGVSGDVFSFVQKIENITFPEAVRLVAQ
                     KLGIPLPKASYSTPGEAKEARLRAQLLDVHERAVAFFQECLRRPEGARAREYLLGRGL
                     NEETTAKFRIGYAPDSGFLLRDRLRGEFSEEVLRESGLFSWKDASPESQVPSPKNPEV
                     GSQLATRDSGLTMYSKFRNRVMFPIASEAGKVIAFTGRTLAADEKSGPKYLNSPETPI
                     YSKSKVLFNLDLAKEWIRKFDYAILVEGQMDCISVFAAGFHNVIASSGTAFTELQAKL
                     LGRFSKNVVVNFDPDTAGAKATERTLGLLVEEEFTIKVLTLEQGFDPDLYIRRKGKDA
                     YAEALRHSQRYFDYLIERARTQFPVRSGEGKDKAVKYLLPHIQRVPSRIVRDELAQEI
                     AQKLGIDSPVLRQELRHVAVTRSAATVKAPAELQITDAEKILIRALASARQMQSAEEH
                     LSMRDGAEEEFDPARQAAFVLQKEQLHRGLATESLAESLLNAGPEVADVMEVPAEEAD
                     RRLLATILLKEDEDLTPDLLDSAVRALRRIHLRRRQEEVQRELKKPGLAANLAANKDR
                     LRDLLAELERISLALRDPGLADAAKRTA"
     gene            2460..>3112
                     /locus_tag="VEJ00_01960"
     CDS             2460..>3112
                     /locus_tag="VEJ00_01960"
                     /inference="COORDINATES: protein motif:HMM:NF015912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HXW99949.1"
                     /translation="MALDDKFDDIKKLIDTGKEKGYLTYDQVNDLIPQDVHSPEDLDD
                     LLTTIGTQGIDVLEGPKPPSSALDRKFEEGEEGEDVELDLSPGALEKTNDPVRMYLRE
                     MGTVPLLTREGEVEIAKRIERGQLRVLKALSRSPIVIRELLTLGEDLKKNVRSIKEVV
                     TFDEEEITDEILQNRLNDFTDKIDDMAKLYKKTNVLEEKFIAVSQKKRPKDHRRARR"
BASE COUNT          690 a         1034 c          891 g          497 t
ORIGIN      
        1 ccgtcgtttt ccgtgcacgc cacgcgccag ttctatcact gcttcggctg cggcgtgtcc
       61 ggagacgtct tcagtttcgt gcagaagatc gaaaacatca ccttccctga agccgtccgc
      121 ctcgtcgcac agaagctcgg catcccgctg cccaaggcga gctactcgac tcccggagaa
      181 gccaaagaag cccgtctgcg cgcccagctg ctcgacgtgc acgagcgtgc cgtagcgttc
      241 tttcaggaat gcctgcgccg tcccgagggc gcccgggccc gcgaatatct cttggggaga
      301 gggctaaacg aagaaacgac cgccaaattc cggatcggct acgcccccga ttccggcttc
      361 ctgctccgcg accgcctccg cggcgaattc agcgaagaag tcctccgcga aagcggtctg
      421 ttttcgtgga aagatgcgag tcccgaatcc caagtcccaa gtcccaagaa ccccgaggtt
      481 ggctcgcaac tcgcgactcg cgactcggga ctaacgatgt actccaaatt ccgcaaccgc
      541 gtcatgttcc ccatcgccag cgaggcgggc aaagtcatcg ccttcaccgg ccgcaccctc
      601 gccgccgacg aaaaatctgg ccccaaatac ctgaactcgc ccgaaacccc catctactcg
      661 aagagcaaag tcctcttcaa tctcgatctg gccaaagaat ggattcgcaa gttcgactat
      721 gccatcctgg tcgagggcca gatggattgc atctccgtct tcgccgccgg attccacaac
      781 gtgatcgcca gctccggcac agccttcacc gagctgcagg ccaagctgct cggtcgcttc
      841 agcaagaacg tggtcgtgaa cttcgatccc gacacggccg gtgcgaaagc caccgagcgc
      901 actctgggac tgctcgtcga agaggaattc accatcaagg tgctgacgct cgagcagggc
      961 ttcgatcccg atctctacat ccgccgcaaa ggcaaggacg cctacgccga ggccctgcgc
     1021 cattcgcagc gctacttcga ttacctgatc gagcgcgccc gcacgcagtt ccccgtgcgt
     1081 agcggcgagg gcaaagacaa agccgtcaag tatctgctgc cccacatcca gcgcgtgccc
     1141 agccgcatcg ttcgcgacga actcgcccag gagatcgccc agaaactcgg catcgattcc
     1201 cccgtcctcc gtcaggaatt gcgtcacgtc gccgtcaccc gctctgcggc caccgtcaaa
     1261 gcccccgccg aactccagat caccgacgcc gaaaagatcc tgatccgcgc cctggcctcg
     1321 gcccgccaga tgcaatccgc ggaagagcat ctgtcgatgc gcgatggcgc cgaagaggaa
     1381 ttcgaccccg ctcgtcaggc cgccttcgtg ctgcagaagg agcagctgca tcgcggcctg
     1441 gctactgaat cgctggccga gtccctcctc aacgccggcc ccgaggttgc cgatgtgatg
     1501 gaggtaccgg cggaagaagc cgatcgccgc ctgctggcga caatcctgct gaaggaggac
     1561 gaagacctga cgcccgacct gctggactca gccgtccgtg ccctgcgccg cattcatctg
     1621 cgccgccggc aggaggaggt ccagcgcgag ctgaaaaagc ccggcctggc cgcaaacctc
     1681 gccgcaaaca aagatcgttt gcgcgatctg ttggcagagc tcgagcgcat cagcctcgcc
     1741 ctgcgcgatc ccggcctggc tgacgcggcg aagagaacgg catgaggaag aatcgtggag
     1801 cgccggacgc ctcgtccccc gtgcgacatt gtgagcaggg cggggcggga ttcaccgtag
     1861 ccaggctgtc ccacctgcag cctgcctgga gcgaggtcga agggcggttt ttacgcgggc
     1921 gacccaccga aactgaggct gccccgcccc ttcgactgcg ctcagggcag cctcttcgcg
     1981 gttttcgaag gatgggagtg atgctgccgg cggcgttcga tgttcagggt cccggaggga
     2041 tccccgacaa tagcccgcca gtccactggc gggaagacag cacagcgacg gatcccgtgc
     2101 cggaggcacg gatgaaacgc gtagggaaac tggccagggc atcgggcgat tggaaagggc
     2161 gcgactttga accgcgtcgc cgagacaggg ggcacgacaa cgggctctag ccccttaagg
     2221 tctgaaacaa aaagacccga aaaggcatct aagtatctaa caggaagaga gataaggtcg
     2281 gggacgggcg gatcgggcgg gtaccggata gacgggtcca cgggaatatg ctaaactcta
     2341 taagtttctg ctggatgcag aatcccgcaa caccccagat tcccaccttt caggaaactt
     2401 tattgcacgc tccgcagcct ggatccgcgg cgcggcaaca accaatttaa ggaaacttct
     2461 tggctctaga cgacaaattc gacgacatta agaagctgat cgacaccggc aaagaaaagg
     2521 gatacctcac ctacgaccag gtgaacgatc tcatccctca ggacgtgcac agtcccgaag
     2581 acctggacga tctgctcacc accatcggta cgcagggcat cgacgtgctg gaaggcccca
     2641 agccgccgtc ctccgcgctc gatcgcaagt tcgaagaggg cgaagagggc gaggacgtcg
     2701 aactcgacct tagcccgggc gcgctcgaga aaaccaacga ccccgtccgc atgtatctgc
     2761 gcgagatggg caccgtcccg ctgctcacgc gcgaaggcga agtcgaaatc gccaaacgca
     2821 tcgaacgcgg ccagctgcgc gtgctcaagg cgctctcgcg ctcgcccatc gtcatccgcg
     2881 agctgcttac gctcggcgaa gacctcaaga agaacgtacg ctcgatcaag gaagtggtca
     2941 ccttcgacga agaagagatc accgacgaga tcctgcagaa ccgcctgaac gacttcaccg
     3001 acaagatcga cgacatggcc aagctgtaca aaaagaccaa tgtcctcgaa gaaaaattca
     3061 tcgcggtctc gcagaaaaag cgccccaagg atcaccgccg cgcccgccgc ag
//
LOCUS       DASPUL010000084         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_12999, whole genome shotgun sequence.
ACCESSION   DASPUL010000084 DASPUL010000000
VERSION     DASPUL010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12999"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VEJ00_01965"
     CDS             <1..248
                     /locus_tag="VEJ00_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HXW99950.1"
                     /translation="THLGMDLEIGEILVTGKSVFAGKTIESSRIRQERGVIVLAIKRR
                     DSMRFNPPPDERIEADDCLIAMGEPAQLRQLEQSASS"
     gene            481..2049
                     /locus_tag="VEJ00_01970"
     CDS             481..2049
                     /locus_tag="VEJ00_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HXW99951.1"
                     /translation="MKIVTAAEMRAIDSATSERFGVPSLTLMENAGAAVAEHVLTHHS
                     AARRIVVFCGKGNNGGDGFVAARRLHQKGKTVQVILLADPTTLRGDAAVMYGKLPVAA
                     IIVHSSEELKSDRVRLSLPADLYIDAILGTGFTPPVSGLYADAIAVLNAAQAPVIAVD
                     IPSGADADAMAPQKSVIARADSIVTFTAARPAHVFSLLTNGPTYVAGIGSPAEAVVSS
                     LQLNVITARDFAPLIDRRPMESNKGNYGHVLVVGGSVGKAGAAAMAGMSALRAGAGLA
                     TVATPKSVLPTVAGFHAEVMTEPLPETEAGTIAANAMERIDALGKGKSVLAIGPGISR
                     FPQTAGLVRALVAKCELPVVLDADGLNAFEGHTDELNGKGRLLVITPHPGEMARLAGC
                     SVADVQKDRLGVARRFSREHEVVVVLKGHRTLVVRPDGEAWANTTGNPGMSTGGTGDI
                     LTGMVAAMIGQHANDTLLAILAAVHLHGLAGDVMRESVGEHSMVATDLLRGLPEAFRR
                     ARGTAREKFVRWDG"
BASE COUNT          469 a          682 c          820 g          481 t
ORIGIN      
        1 ccacacacct gggaatggat ctggaaatcg gggagattct ggtgacgggc aaatcggtgt
       61 tcgccggcaa gaccattgaa agctcgcgga tccggcagga gcggggagtg atcgtattgg
      121 ccatcaagcg gcgcgacagt atgcgcttca atcctccgcc tgacgagcgc attgaagcgg
      181 acgactgcct gatcgccatg ggcgagccgg cgcagctgcg gcaactggag cagagtgcga
      241 gttcatgaaa ctctcagcgc gattgaccag gcggaaatct gatctcgttc gagtcatttc
      301 gttggcgtca tcccgagcgc agccgttttt caggcggagc gagggatctc ccaaaccggc
      361 ccggttgcag ctgagatccc tcggcccgct tgcaaaagcg cgggccttgg ggatgacgcc
      421 ctcaaagaat gatttcacac caatccatac cagctcagga tctgggacct tggcttcgct
      481 atgaagatcg ttaccgcggc ggaaatgcgc gccatcgaca gcgccaccag cgagcgcttc
      541 ggcgtgcctt ccttgacgct gatggaaaac gccggggcgg cggtggccga gcacgtgctc
      601 acgcatcata gtgccgcccg gcgcatcgtc gttttctgcg gcaagggcaa caacggcggc
      661 gacggcttcg tggcggcgcg gcggctgcac cagaagggga agacggtgca ggtcatcctg
      721 ctggctgatc cgacaaccct gcgtggagat gccgccgtca tgtatggcaa actgccggtg
      781 gcggcgatca tcgttcactc cagcgaagag ttgaagtcgg accgcgtgcg gctctcgctg
      841 cccgcggatt tgtatatcga cgcgattctg ggcaccggct tcactccgcc ggtcagcggg
      901 ttatacgccg acgccatcgc tgttctgaat gcggctcagg cgccggtgat cgcggtcgac
      961 attccctcgg gtgccgatgc tgacgcaatg gcacctcaga agagtgtcat cgcgcgcgcg
     1021 gattcgatcg tcaccttcac ggccgcgcga cctgcgcatg ttttcagctt gctgacgaac
     1081 gggccgactt atgttgcagg aatcggctcg cccgcggagg ccgtcgtttc gtcgctgcaa
     1141 ctgaacgtga tcaccgctcg cgactttgcg ccactgatcg accgtcgtcc gatggaatcg
     1201 aacaaaggca actacgggca tgtgctggtg gtcggagggt cggtaggcaa ggcgggagca
     1261 gcggcgatgg ctggaatgtc ggcattgcgc gccggggccg ggctggccac agttgccaca
     1321 ccgaagtccg tgttgccgac ggtggctggg tttcatgccg aggtcatgac cgagccgttg
     1381 ccggaaaccg aggcaggcac gattgccgca aacgcaatgg aacggattga tgcgttgggc
     1441 aaagggaaaa gcgttctggc gatcgggccg gggatttcgc gctttcccca gacggccggc
     1501 ctggttcggg cactggtcgc caagtgcgag cttcctgtcg tcctggacgc cgacggcctg
     1561 aatgcattcg agggacatac tgatgaactg aacggcaaag ggcggctgct ggtgatcacg
     1621 ccgcatcccg gtgagatggc gcggctggcg ggctgctccg tcgccgatgt gcaaaaggat
     1681 cggcttggcg tggcgcggag gttctcgcgc gagcatgagg tggtcgtggt gctgaaggga
     1741 catcgaacgc tggttgttcg accggacggg gaagcatggg ctaatacgac cggaaatccc
     1801 ggcatgtcga cgggcggaac gggcgacatt ctgaccggca tggttgctgc catgatcggc
     1861 cagcacgcga acgacactct gctcgcgatt ctggcggcgg ttcatctgca tggactggcg
     1921 ggcgacgtga tgcgcgagag cgtgggcgag cattccatgg ttgcgaccga tctgctgcgt
     1981 gggctgccgg aggcttttcg ccgggcacgg ggcacggcgc gggaaaaatt cgtgcgctgg
     2041 gatggatgat tgctccgcgt ctgtcatcct gagcggaaga tgtcctgcgc ggaagcagga
     2101 catgcggagt cgaaggatct cgtctttgta tgcagtggca tcggcctcat aaggggttct
     2161 ctcggcgccg cgcgtatcgt gagaattccc tgcaacggct ggttagctga aaaggcaaat
     2221 agggatcctt cgacttagcc aacacttcgc tgcgcgaagc attggctgcg ctcaggatga
     2281 cattcgaaag ggacaggttg tatttcccaa gaagatatgt cctgccgtac attaactaat
     2341 tagtaattag tcgctaaatg cttcttggca ttggatctgc aacccatcag ttcaggcctc
     2401 ccatgcggaa ttcggacgat gggcctcagt aatctattac gcaacagggg at
//
LOCUS       DASPUL010000085         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_697671, whole genome shotgun sequence.
ACCESSION   DASPUL010000085 DASPUL010000000
VERSION     DASPUL010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697671"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VEJ00_01975"
     CDS             complement(<1..713)
                     /locus_tag="VEJ00_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXW99952.1"
                     /translation="MADTILVVVEQREGKLNRVSWETITAGQAISAATGWGLEAAVVG
                     SGIASVGAEVAAKKVAKVYAVESPKLEPYTPDAFAAGLKQFLSTKQPRLVLMPHTYQV
                     RDFIPKLVTAMGRTVISDCIGFKYEGGKLLFTRQMFQGKLAADVSFGSDGPWFATFQN
                     GAFRGDQVQEGANASPVETVTVEIGDNVVRSKPQPPFKEAKQAVDLTQAEIIVSVGRG
                     IKEQKNIELAKQLADALGG"
     gene            complement(865..1635)
                     /locus_tag="VEJ00_01980"
     CDS             complement(865..1635)
                     /locus_tag="VEJ00_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HXW99953.1"
                     /translation="MKILVCLKQVPQKDAPLKLNDAATWIREDISYEVNEPDAYALEE
                     ALRQKEKHTGEVVVITSGPARAQQVLREALAKGADRAIHLEDDKFVGLDAFNTARALA
                     AAIKDEKFDLIFTGLQSDDYGYAQTGVILAELLGWPHATIIMQIEKTDSGIRVKRELE
                     AGYFQFVDMPTPAVLTIQSGINKLRYATLIGIKQAKNKPMRKVGLAEVQSAVGENLQR
                     IERLYVPQKTKKTEVIEGPPAEVAKKVVDKLRNELRVL"
     gene            1785..2489
                     /locus_tag="VEJ00_01985"
     CDS             1785..2489
                     /locus_tag="VEJ00_01985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HXW99954.1"
                     /translation="MPDDTFSAMRQSIVDGAPDTASGLAQQAVAAGVAPLDAINHGYV
                     PGMHDVGEQFARGQMFLPDMMASAEAMRSAMAILDPELRKAGTERPMAGVVVLGTTKG
                     DIHEIGKILVGTLLTAHGFRVHDLGVDVPGEAFASNARELNADIVGVSALLTTTMRNQ
                     KGVVEALERAGLRSQVKVMVGGAPVTRRWAEEIGADGYAKDAMSAVALAQELMQQKLQ
                     IAVPEVCSAQPWKSGP"
     gene            2544..>2893
                     /locus_tag="VEJ00_01990"
     CDS             2544..>2893
                     /locus_tag="VEJ00_01990"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HXW99955.1"
                     /translation="MRPKLELLDKPFIERILDEAFQLIEDPGVRVAPYVVDLLRGAGI
                     TVKDGVAHIPEPLARRLLDLAPRGFCLYNRSGAPTVRYGGDDVHFDPGSSCLNILDPE
                     TQQARPALSADLVRL"
BASE COUNT          514 a          965 c          803 g          611 t
ORIGIN      
        1 tctccaccca gcgcgtcggc cagttgcttc gcaagttcga tgtttttctg ctccttgatc
       61 ccgcggccca ccgaaacaat gatctcggcc tgcgtgaggt cgaccgcctg cttggcctct
      121 ttgaacggcg gttgcggctt cgagcgcacc acgttgtcgc caatttcgac cgtcacggtc
      181 tcaaccggcg aggcgttggc cccttcctgc acctgatcgc cgcggaacgc cccattctga
      241 aaggtcgcga accatggccc gtcgctgcca aaacttacgt ccgcagccag cttgccctgg
      301 aacatctgcc gcgtgaacag caacttcccg ccctcgtact tgaagccgat gcaatcgctg
      361 ataacagtcc gccccatggc tgtcactagt ttcgggatga agtcacgcac ctggtaggtg
      421 tgcggcatca gaaccagtcg gggctgcttc gtgctcagga actgcttgag tcccgccgcg
      481 aaagcgtccg gtgtgtaagg ctcgagcttc ggtgattcga ccgcgtacac cttcgcgacc
      541 ttcttcgccg caacttcagc gccgacagac gcgatcccgc tgcccacaac cgctgcttcc
      601 aatccccagc ccgtcgcggc agaaattgcc tgccccgcag taatggtttc ccaggaaacg
      661 cgattcagct tgccttcgcg ctgttcgact actaccagaa tcgtatctgc cataagattt
      721 tcagtccccg aactctagag gcatgttgtc attccgaacc ggcgtaagcc ggtgaggaac
      781 ctgcttttct gcctgtgccg ccaaaaattc gccagctccc gctcggcccg gacgggcgag
      841 gtgcccgtcc cacacggcct gtgcttatag cacccgtaac tcgttccgca gcttgtccac
      901 cactttcttg gcgacctctg ccggtggtcc ttcgatcact tccgttttct tcgtcttctg
      961 cggcacgtag agccgttcga tcctctgcag gttctcgccc accgccgact gcacttcggc
     1021 gagccctact ttccgcatcg gcttgttctt ggcctgcttg atgccgatca gcgtcgcata
     1081 gcgcagcttg ttaatgcctg actgaatcgt cagcacagcc ggcgtcggca tgtcgacgaa
     1141 ctgaaagtat cccgcttcga gttcgcgctt cacgcgaatc ccgctgtcgg tcttctcgat
     1201 ctgcatgatg atggttgcgt gcggccaccc cagcaattca gccagaatca ctccggtctg
     1261 cgcgtaaccg tagtcgtcgg actgcagccc ggtgaagatc agatcgaact tttcgtcctt
     1321 gatcgccgcg gccaaagccc tggccgtatt gaaggcatcc agcccgacaa acttgtcatc
     1381 ctcgaggtga atggcccggt ccgcgccctt ggccagcgcc tcgcgcaaga cctgctgggc
     1441 gcgcgccggc ccgctggtga tcaccaccac ttctccggtg tgcttttcct tctggcgcag
     1501 cgcctcttcc agcgcatacg cgtcgggctc gttcacttcg taggagatgt cttcgcggat
     1561 ccaggtggcc gcgtcgttca gcttgagcgg agcatccttc tgcggcacct gcttcaggca
     1621 aaccaggatt ttcaagatca ggactcctcg cctgtaacct cagtacggtt acagcaaaac
     1681 tggtaagtat aaatgagcca gtcctgtgct tggaaacgcc cgcggtgtga tcccaatcac
     1741 tgaccggtcc cacgcgcaca agtcagcatg gcccaaggcc atttatgcct gacgacactt
     1801 tctccgccat gcgccaaagc attgtcgatg gcgcgcctga caccgcctcc ggccttgccc
     1861 agcaggctgt tgccgcaggc gttgccccgc ttgacgccat caaccacggc tacgttcccg
     1921 gcatgcacga cgtcggcgag caatttgccc gcgggcagat gttcttgccc gacatgatgg
     1981 cttcggccga ggccatgcgc tcagccatgg ccatactcga tcctgaactg cgcaaggcag
     2041 gaaccgagcg ccccatggcc ggagtcgtcg tgctgggaac gacgaagggc gacatccacg
     2101 aaatcggcaa gattctcgtc ggaacgctcc tcaccgcgca tggctttcgc gtgcatgatc
     2161 tgggggtgga cgtcccaggc gaggctttcg ccagcaacgc gcgcgaactg aatgccgata
     2221 tcgtcggcgt ctccgcgctg ctgaccacca ccatgcgtaa ccaaaaaggc gtggtagagg
     2281 cgctcgaacg ggccggcctg cgctcccagg tcaaggtcat ggtcggtgga gcgcctgtga
     2341 cgcgccggtg ggcggaagaa atcggtgccg acggctacgc caaagacgcc atgagcgccg
     2401 tcgcgctggc gcaggaactg atgcagcaga aattgcagat cgcagttcct gaggtttgct
     2461 ctgcgcaacc gtggaagagc ggcccttgag ggccgcgtaa agcgtcttga agaatggggg
     2521 ctttagcccc tgagtcacac tgcatgagac ccaagctcga actgctcgac aagccgttca
     2581 ttgaacgtat cctcgacgag gcttttcagc tcatcgagga tcccggtgtg cgtgtcgcgc
     2641 cctacgttgt cgatctgctg cgcggcgcgg gcatcacggt taaagatggt gttgcgcaca
     2701 tccccgagcc tctggcgcgc cgcttgctcg acctcgcccc ccgcggcttc tgcctctaca
     2761 atcgcagcgg tgcccccacc gtccgttatg gcggagacga tgttcatttc gatcccggct
     2821 catcgtgcct gaacatcctc gatcctgaaa cccagcaagc acgtcccgcg ctgtcggctg
     2881 atctcgtgcg cct
//
LOCUS       DASPUL010000086         3821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1520753, whole genome shotgun sequence.
ACCESSION   DASPUL010000086 DASPUL010000000
VERSION     DASPUL010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520753"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VEJ00_01995"
     CDS             <1..188
                     /locus_tag="VEJ00_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99956.1"
                     /translation="EVTVPELLSGGERSRQEEISELMKDQFIAELLPFVTQLNGMQMS
                     SILTQVRDLTVRPRRTA"
     gene            771..1838
                     /locus_tag="VEJ00_02000"
     CDS             771..1838
                     /locus_tag="VEJ00_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXW99957.1"
                     /translation="MNLEQTTPAFLGNTGSLRHFDDSTPERSSTGSTTDAPLLDAYSQ
                     AVTGAVERISPSVVNIEVHQSGGRSRSGQPRERSGGGSGFVFTPDGLILTNSHVVHDA
                     ARIAVTLADGCRMPASLIGDDPASDLAVVRIEKPRVDEPGLIAALLGDSQRLRVGQIV
                     IAIGAPYGFQSTVTAGVVSALGRSLRSYSGRLIDDVIQTDAALNPGNSGGPLVDSAGR
                     VVGVNTATILPAQGICFAIGINTAKFVASRLLRDGRLRRSYIGVSAQTVPLHRRVVRF
                     YDLPKESGALVLLVEDASPARRAGLREGDVIVALEGQPVAGVDDLHRLLTEVRVGLSS
                     TLTVVRYTEKLELKIVPEEAK"
     gene            2024..2830
                     /locus_tag="VEJ00_02005"
     CDS             2024..2830
                     /locus_tag="VEJ00_02005"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HXW99958.1"
                     /translation="MPTLHDKVDKVPAGLDRKETEVYRHLDPARLPRHIAIIMDGNGR
                     WARRRHMPRIAGHRAGVAAVRSTVETAARIHIPALTLYAFSEENWKKRPTSEVGFLMR
                     LLSRYLKAEVPTLNENNIRLEYIGRRHELPADVQERMAWAQENTARNSGMVLTLALNY
                     SARSELVDAFCSIVDAASQNGGLHHLKIDEDAISRNLYTRHLPDPDLVVRTSGEMRLS
                     NFLLWQLAYAEIYVTPTLWPDFRGVHLLEGIAEYQKRERRYGGLNGGQTS"
     gene            3016..>3821
                     /locus_tag="VEJ00_02010"
     CDS             3016..>3821
                     /locus_tag="VEJ00_02010"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXW99959.1"
                     /translation="MLKRIVTAVVLIPIVLALILRAPVYVLAIVAGIVALLTIHEFLK
                     LTEAYGVQPMAWPTHLFAALLFLTLAIDAAADTPLLSTGKFLVSLAFLTGIVPFVFLT
                     RAMRKDDLRTGYPAAAASVFAFTYIALPMAMLAQLRQQWAGGFYLLYLLLVVWAGDIF
                     AYFVGKAIGRHLMAPRISPKKTWEGAVASLAASVAVGWMLFHYALPISSALLRAGLIA
                     RRDGLFALEPPAIGPVILLTILLNLAAQLGDLVESLIKRGAGAKDSGSILP"
BASE COUNT          708 a         1307 c         1073 g          733 t
ORIGIN      
        1 tggaggtcac cgtgcccgaa ctgctcagcg gcggcgaacg cagccgccag gaagagatca
       61 gcgaactgat gaaagaccag ttcatcgccg agctgctccc gttcgtcacg cagctcaacg
      121 gcatgcagat gtcgagcatc ctgacccagg tgcgggattt gacggtgcgc ccgagacgga
      181 ccgcgtagct caagcttcct cttcgtgagc cgatggaagc attggtgccg agaaacgctg
      241 agtagaggct tgtatcagac ctctcgaacc gccgtggaag agcggcgctt cagcgccgcg
      301 taaggcggcc caaggcgggg gctttagccc ctggctcgaa caaacgattt ttatccgggc
      361 aaccagccaa cactctgccg ggaccatcct ctggtcccgg cgtttttatg ctccgtgttc
      421 tgtgcagcgc tcgttttggt ggaagagtgg cgcttcagcg cccgtgagca tcatcacttc
      481 ttttgtcatc ctgagcggag gaacaggttc gcgaatgcaa gcctgttccg cagtcgaagg
      541 atcccttgca cttccgcact gcccgagccg aagagaggaa tctccaagca actctccgcc
      601 ccgcggctcc gtggccgaaa ctcctgagaa ggcgcaagta gtgccgccta cagggatcct
      661 tcgactgcgt agttgtccgc cgctgcggac aactactttg ctcaggatga caaaaccagg
      721 agagtccaag ccgtctgaat ccaaccgata tcgaccaaca tctatcctcc atgaatctgg
      781 aacaaaccac accggccttc cttgggaata ccggttccct ccgccatttt gatgactcca
      841 caccggaacg ctccagcact ggaagcacca ccgacgcccc gctcctcgac gcctactcgc
      901 aagccgtcac gggcgcggtc gagcgcatca gtccatccgt cgtcaatatc gaagtccacc
      961 agagtggcgg ccgcagccgt tccggtcagc cccgcgagcg gagtggcgga ggctcaggct
     1021 tcgtcttcac ccccgacggc ctaatcctca ccaacagcca cgtcgtgcac gatgctgccc
     1081 gcattgccgt cacgctggcc gacggatgcc gcatgccggc ttcgctgatc ggcgatgatc
     1141 ccgccagcga tctggcggta gtccgtatcg aaaagccgcg cgtggacgag cccggcctga
     1201 tcgcggctct ccttggcgat tcccagcgcc tgcgcgtggg ccagatcgtc atcgccattg
     1261 gcgcgcccta cggatttcag tccaccgtta cggcgggcgt ggttagcgcc ctgggccggt
     1321 cgctgcgctc ctactccggc cgcctgatcg acgacgtcat tcaaactgac gccgcgctca
     1381 accccggcaa ctcgggcggg ccgctggtcg attccgccgg ccgcgtggtc ggcgtgaaca
     1441 cagcaacgat tctgcccgct cagggcatct gcttcgccat cggcatcaac accgccaaat
     1501 tcgtggccag ccgactgctg cgcgatggac gcctgcgccg cagctacatc ggcgtctcag
     1561 cccagaccgt acctcttcat cgccgcgtcg tccgcttcta cgatctgccg aaggagagcg
     1621 gcgcactggt gttgttggtc gaagacgcca gcccggccag gcgcgccggc ctgcgcgaag
     1681 gagacgtcat cgtcgcactc gaaggccagc cagtagccgg cgtagatgac ctgcatcgcc
     1741 tcctgacaga ggtccgcgta ggattaagca gcacgctgac cgtggtgcgg tatacggaga
     1801 agctggaact gaaaatcgtg ccagaggaag caaagtaagc cggtgtggcg cgggcgccct
     1861 cgcccgcgtg ccggcagcgc tccaatccga gtcgttcaaa tttagcctaa gtcctttgca
     1921 aagatcggag tgccagacac tttcggcagc cgcgggcagg agtgcccgcg ccacacaacc
     1981 ctcccaattg catccccgtc attactgtgt tagcctctat ctcgtgccca ctttgcacga
     2041 caaagtagac aaagtccccg cgggtctcga ccgcaaggaa actgaagtct accgccatct
     2101 cgatcccgcc cgtctccctc ggcacatcgc catcatcatg gacggcaacg gacgctgggc
     2161 caggcgtcgc cacatgcccc gcatcgccgg acatcgcgcc ggagtcgccg ccgttcgctc
     2221 caccgttgaa accgctgccc gcatccacat tcccgcgctc acgctctacg ccttttccga
     2281 agagaactgg aagaagcgcc cgaccagcga agtcggcttc ctcatgcgcc tgctcagccg
     2341 ctacctgaaa gccgaagtcc ccacgctcaa cgagaacaac atccgcctgg aatacatcgg
     2401 ccgccgccac gaactgcccg ccgacgtaca ggaaagaatg gcctgggcac aggaaaacac
     2461 agcccgcaac tccggcatgg tgctgaccct ggcgttgaac tacagcgccc gcagcgaact
     2521 ggtcgacgcc ttctgctcca tcgtcgatgc tgcctcacag aacggcggcc tccaccatct
     2581 gaagatcgac gaagacgcta tcagccgcaa tctttacacg cgtcacctgc ctgacccaga
     2641 cctggtcgtc cgcacctccg gcgagatgcg cctgagcaac tttctgctgt ggcagctggc
     2701 ctacgctgag atctatgtga cgccgacgct atggcccgat ttccgcgggg tccatctcct
     2761 cgaaggcatt gccgagtacc agaagcgcga acgccgctac ggcgggttga acggcgggca
     2821 gacgtcctaa gcgcgctcag tcgtagcttt gaggggtcgc gcctttcagg cgcgacgtgg
     2881 tgttcttctt tgaaatcacc ttgtggcgcg gctggaagcc gcgacctttc aaaacgtcag
     2941 tccacggtgg tcgaaacgct tcaattcgtg gcaagatcgt aggcgcagtt tcgactccat
     3001 acccatcgat atcccttgct caaacgcatc gtgacggccg ttgtactcat tcccatcgtg
     3061 ctggcgctca tcctgcgcgc gccggtatat gtgctggcaa tcgtcgccgg catcgtcgcg
     3121 ctgctgacca ttcacgaatt cctgaagctg actgaagcct acggagtcca gccgatggcc
     3181 tggccgactc accttttcgc ggcgctactc ttcctcaccc tggcgatcga cgcagccgcc
     3241 gatactcctc tgctctcgac cggaaagttt ctggtttctc tcgctttcct taccggcatc
     3301 gttccttttg tcttcctgac ccgcgccatg cgtaaggacg atctgcgaac cggctacccc
     3361 gccgccgcag cctcggtctt cgcgtttacc tacatcgcgc ttccgatggc catgctcgcc
     3421 caactccggc agcaatgggc gggtgggttc tatctccttt atttgctgct ggtggtttgg
     3481 gccggcgaca tcttcgccta cttcgtcggc aaagcgatcg gccgacatct catggccccg
     3541 cgcatcagtc cgaagaagac ctgggaaggc gcagtcgcct cattggcggc cagtgttgcc
     3601 gtcggatgga tgcttttcca ctatgccctg ccgatcagct ccgccctgct ccgcgcgggg
     3661 ctgatcgcgc gccgcgacgg cctattcgcc ttggagccac cggcgatagg gcccgtgatc
     3721 ctgctgacga tcttgctcaa cctcgccgcc caacttggcg atctggtcga gtcgctgatc
     3781 aagcgcggcg ccggtgcaaa agactcgggc tcgatcctcc c
//
LOCUS       DASPUL010000087         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_698248, whole genome shotgun sequence.
ACCESSION   DASPUL010000087 DASPUL010000000
VERSION     DASPUL010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698248"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VEJ00_02015"
     CDS             <1..441
                     /locus_tag="VEJ00_02015"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HXW99960.1"
                     /translation="PLPSDSEIGPCTLNIGQIEGGRAPNVIPDYAHADLLYRLVGASD
                     ELRRQIVSTVGNQVEVTFPLEIPFQRLRTVDGPPTMIAAFTTDIPFLGNWGEPLLIGP
                     GSIHVAHTEREHIEKRQLAEAIDLYCRIAKALVARTTSEAAANG"
     gene            604..1563
                     /locus_tag="VEJ00_02020"
     CDS             604..1563
                     /locus_tag="VEJ00_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99961.1"
                     /translation="MLDQAKIAEILQAARDKAETILDLRDKARAQRAQALEANQKKMA
                     LQIRLAAPGVESAATVETAGFLAAAGDSWFDYPLHDVLKLLDDRYGYNVESSAHKGDP
                     IEKMAYPGGQMEDFARKLDKIKDRGAVPKAVLVSGGGDDVAGKEFGMLLNNAFSRIAG
                     WDPEVVDGVVNVRIKTAYLDMLMTITHMCESRFDGKLIPILVHGYDYPVPDGRGFLGG
                     WPFPGPWLDPGFREKNFPNDSPADLQKRIAMMKTTMDQFNTMVGSLPSQPELAHVHYI
                     DLRGTLSTQLSDYKDWWANELHPTEKGFNAVTDKFAAVLAKLP"
     gene            complement(1696..4425)
                     /locus_tag="VEJ00_02025"
     CDS             complement(1696..4425)
                     /locus_tag="VEJ00_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXW99962.1"
                     /translation="MLGQTISHYRILEKLGGGGMGVVYKAEDTELGRFVALKFLPDDL
                     AADPQALERFRREARAASALNHPNICTIHEIGRHEGHSFIVMEFLEGVTLKHRIAGRP
                     LDLETLLGLAIEIADALDAAHAKGIVHRDIKPANIFVTAHGHAKVLDFGLAKFSPVAG
                     PGSPGQGETRSVDEQHLTSPGSAVGTVAYMSPEQIRGKELDPRTDLFSFGAVLYEMST
                     GALPFRGDTTGVIFDSILNRPFASPARLNPDLPADVERIIDKALEKDRELRYQHAADM
                     RSDLKRVKRDSGSARDHSRPAAAAAPAEPAASSQARVSDAVPVAPSGTTQSSSSAVLV
                     AAASRNKGKVFALGAVLILLVLAAGYGAYHLLSGRRAPAGQGTITQISHWHKPVSNAL
                     LSPDGHTIAFTSYLQGYEQIFIMLTSGGDPLQLTSDEGSKTLYNFSTDGTQIYYGRNL
                     GAYEVWAIPTLGGTPARVVEGEFALPVSDGKTLYYANPQTRKVMQAGVDGSDPRAAIG
                     FDEMSLVPQQVLVYPEGSDFLVAGATDKTPAGTLQVNRLNLATHKATDLGQIPAAQEL
                     PQWGEPGKTLLFNRDLNGIVNLWEYNLSDRSLTQLTFGPGPDSFPMPDPAGKGIYFVN
                     GRQSGYLSVYDVRGKTSSDIVPEITFQPTLSHNGKRVMYITAPETGHYEMWVSDVDGG
                     NKTRLATSTDPMATGDWSRDDSQLIFWKGPQSGVEIFAVNGDGTHLREFPRTIAFSNG
                     VTWAPAADAFYISGHLKASDTVTTIWKLPAGSSSLETLAEGCGSVRDVSPDGKYVLTS
                     ELSGNNGIFELSVADKKCTALLPGAMSFFPRFSPDGKSIVYTVSSRGEVIVNRLAWHD
                     GQTAGNPQPVFKLPFAFPQDYSGGNAYDIAPDLSRIVYVRPGGQFDFYLLSRR"
BASE COUNT          898 a         1458 c         1409 g          870 t
ORIGIN      
        1 cccctgccat ccgattctga gatcgggcca tgcacgctga acatcggaca gatcgaaggc
       61 ggccgagctc ccaacgtgat tcccgactac gcccatgccg acttgctgta ccgtcttgtc
      121 ggcgcctccg acgaactgcg ccggcagatt gtctctaccg tcggcaacca ggtggaagtc
      181 acgttcccgc tcgagattcc ttttcaaagg ctgcgcaccg tcgatggtcc gcccacgatg
      241 atcgccgctt tcactacgga catccccttc ctcggcaatt ggggcgaacc cctgctcatc
      301 ggtccgggat cgattcacgt cgcccacacc gagcgtgagc acatcgaaaa acggcaactc
      361 gccgaggcga tcgatctcta ctgtcggatc gcaaaggctc tcgtcgcgcg cacaacgtca
      421 gaagctgcgg caaacgggta acacgcttcc ctctgtcgcc cctgaacctg atttacgttc
      481 tccggacaaa ctacgactcc ctctcccgct ctgctggacc gggcgtatac ttcggccccg
      541 cgcgaactcg gggacgcgcg cccgtcccca caaccgcacg agttccgcga tcaggaggac
      601 accatgctag accaggccaa aatcgcagaa attctgcagg cggcgcgaga caaagcggaa
      661 accatcctgg atttgcgcga caaagcccgc gcccagcgcg cgcaggccct cgaggccaat
      721 caaaagaaga tggcgctgca gattcggctg gcagcgcccg gcgtggagtc ggctgcgacg
      781 gtcgagactg ccggttttct ggcggctgcc ggcgattcct ggttcgacta tccgctgcac
      841 gacgttttga agctgctcga cgaccgttat ggctacaacg tggagtcgtc ggcccacaag
      901 ggcgatccca tagaaaaaat ggcctacccc ggcgggcaga tggaggactt tgcgcgcaag
      961 ctggacaaga tcaaagatcg cggcgccgtc cccaaagcgg ttctggtctc gggcgggggc
     1021 gacgatgttg ccggaaaaga gttcggcatg ctgctcaaca atgccttctc cagaattgcg
     1081 ggctgggacc ccgaggtggt cgatggcgtg gtcaacgtca ggatcaaaac cgcctatctc
     1141 gacatgctca tgaccatcac gcacatgtgc gagagccggt ttgacggcaa gctcataccg
     1201 atcctggtgc acgggtacga ctatcccgtc cccgacggcc gtggattcct cggaggctgg
     1261 ccgtttcccg gaccctggct cgaccccgga tttcgcgaaa agaattttcc caacgacagc
     1321 cccgccgatc tgcagaagcg catcgccatg atgaagacca cgatggacca gttcaacacg
     1381 atggttgggt ctctcccgtc acagcccgaa ctggcgcatg tccattacat cgatctgcgg
     1441 ggcaccctgt cgacgcagct atccgattac aaagactggt gggcgaacga acttcatccg
     1501 acggaaaagg gattcaatgc cgtgaccgac aagtttgccg ccgtgctggc gaagcttccc
     1561 tagcagtgtg gcgaaatact cgcttcaggt tgcgttttgc gttttgaaag ggcgcggctt
     1621 ctagctgcgc cctggatacc gcaaactcaa gcacggcttt agccgctgag ggcgattcct
     1681 gccgccagca accttctacc tacgcgacag caggtagaag tcaaactgcc cgccggggcg
     1741 gacgtagacg atcctggaaa ggtcgggggc gatgtcgtac gcgttgccgc cgctgtagtc
     1801 ctgcggaaac gcgaacggca gtttgaagac cggttgcggg ttgcccgcag tttggccgtc
     1861 gtgccaggcc aggcgattga cgatgacctc gccgcgcgag gaaaccgtat acacaatgga
     1921 tttgccgtcg gggctgaagc gcgggaaaaa actcatcgcg cccggcagca gggcggtgca
     1981 tttcttgtcg gcaaccgaca gttcgaagat gccgttgttc cccgagagtt cggaggtcag
     2041 cacgtacttg ccgtcgggcg agacatcccg cacgctgccg catccctcgg ccaatgtttc
     2101 cagcgaagag ctgccggcag ggagcttcca gatggtcgtg acggtgtcag atgccttgag
     2161 atgaccgctg atatagaacg cgtctgccgc gggagcccag gtcactccat tggaaaaggc
     2221 aatagttcgc gggaactctc gcagatgggt cccgtcgcca ttcaccgcaa aaatctccac
     2281 gccgctctgc ggacccttcc agaagatgag ttgtgaatcg tcccgcgacc aatccccggt
     2341 ggccatcgga tcggtagacg tggccagccg ggtcttgttt ccgccatcca catccgagac
     2401 ccacatctca tagtggccgg tctcgggcgc ggtgatgtac atcacgcgct tgccgttgtg
     2461 cgagagcgtc ggctgaaacg taatctccgg tacgatgtcg ctcgaggtct tgccccgcac
     2521 atcgtagaca gagagatatc cggactgcct gccattcacg aagtaaatgc ccttgccggc
     2581 aggatccggc atggggaagg aatccgggcc ggggccgaag gtgagctggg tcaggcttcg
     2641 gtccgagagg ttatattccc acaggttgac gatgccgttc aggtcacggt taaagagcag
     2701 agtcttgccc ggctcgcccc attgcggtag ctcctgagcc gcaggtattt ggccaaggtc
     2761 ggtggccttg tgcgtggcga ggttgaggcg gttgacctgc agcgtgccgg cgggagtctt
     2821 gtcggtagcc ccggctacga gaaagtcgct gccttcgggg taaaccagca cctgctgcgg
     2881 cacaaggctc atctcatcaa atccgatcgc ggctctcggg tcgctgccgt cgacgcccgc
     2941 ttgcatcacc tttcgggtct ggggattcgc gtagtacagg gtcttgccgt cgctcacggg
     3001 cagcgcaaac tccccctcca ccacgcgcgc gggagttcca cctagggtcg ggatcgccca
     3061 cacttcatag gcgcccagat tgcggccata gtagatctgc gtgccatcgg tggagaagtt
     3121 gtacagcgtc ttgctgcctt catcgctggt gagctgcagc gggtccccgc ctgaagtcag
     3181 catgatgaaa atctgttcat agccctgcaa gtaggatgtg aacgcgatcg tgtgcccgtc
     3241 gggcgacagc agggcgttgc tgaccggctt gtgccagtgg ctgatctgcg tgatggttcc
     3301 ctgaccggcg ggagcgcggc ggccggacag aagatgatac gcgccatatc ccgccgccag
     3361 aaccagcagg atcaacaccg cacccagagc aaagactttc cccttgttgc gcgaagccgc
     3421 tgcgaccagc accgcgctgc ttgaggactg agttgtgccg gatggagcga ccggcacggc
     3481 gtctgaaaca cgagcctgtg aagatgccgc cggttcggca ggtgccgctg ccgccgccgg
     3541 tcgcgagtga tcccgagccg agcccgaatc ccgtttcacc cgcttcagat cgctgcgcat
     3601 gtcggccgcg tgctggtagc gcagctcgcg gtctttttcc agcgctttgt cgatgatgcg
     3661 ctccacatcc gccggcaaat ccgggttcag ccgcgccggc gacgcaaacg gtcgattgag
     3721 aatggaatca aaaatgacgc ccgtcgtgtc gccgcggaac ggcagcgcgc cggtcgacat
     3781 ttcataaagc accgcgccaa acgagaacag gtcggtccgt ggatcgagct ccttgcctcg
     3841 aatctgctcg ggcgacatgt aagccacggt gcccacggcg ctgcccgggc tggtcaggtg
     3901 ctgctcgtcg acgctccgcg tctcgccctg ccccgggctg cccgggcccg caaccggcga
     3961 gaatttcgcc agcccgaaat ccagcacctt ggcatgaccg tgcgcagtca cgaaaatgtt
     4021 cgccggcttg atgtcgcggt gcacgatgcc cttggcgtgc gccgcatcca gcgcgtcggc
     4081 gatttcgatg gccagcccca gtagggtctc cagatcgagc ggccgcccgg cgatgcgatg
     4141 cttcagagtg acgccctcga ggaactccat cacgataaag ctgtggcctt catgccgccc
     4201 gatctcgtga atcgtgcaga tgttgggatg attcagcgcc gaagccgccc gcgcctcgcg
     4261 gcgaaaccgt tcgagcgcct gcggatcggc tgccagatcg tcaggcagga acttgagcgc
     4321 cacaaagcgg ccgagttcgg tatcctcggc cttgtagacc acacccatcc cacccccgcc
     4381 cagcttctcg aggatgcgat agtgcgaaat ggtctgcccc agcatgggga gaatggtatg
     4441 tgcggcggag gggcaagtca acgaaaggga aagtgcaagc tattctaaaa ttgaacttct
     4501 cttcgtttcc gatggtgtca tcccgaaggc gcgcactttc accagcgcgc cgagggatct
     4561 cccgtggaat gcacgttgcg cgggagatcc ctcactccgc ctgaaaaccg gctccgctcg
     4621 ggatgacgcc gttcg
//
LOCUS       DASPUL010000088         4528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_425124, whole genome shotgun sequence.
ACCESSION   DASPUL010000088 DASPUL010000000
VERSION     DASPUL010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425124"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VEJ00_02030"
     CDS             <1..544
                     /locus_tag="VEJ00_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HXW99963.1"
                     /translation="AIKFDYKPSTLKIINNGHTIQINYDPGSTITVGGKQYELVQFHF
                     HRPSEEKIDGKSFDMVAHLVHKDSSGNLAVVAVLVRSGQSNSLIATLWSHLPQEKGVE
                     QLVANTQIDAAKLLPSDRAYYTFTGSLTTPPCSEDVTWFVLRDPMQFSKQEVARFAKL
                     YPMNARPVQPLNGRLVETSP"
     gene            complement(1130..2797)
                     /locus_tag="VEJ00_02035"
     CDS             complement(1130..2797)
                     /locus_tag="VEJ00_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXW99964.1"
                     /translation="MTRIPRSFSMVLAGFAVALAAAIPCAGLAQAPVQKPNIILIVSD
                     DFGYGDAGVYGGGPNRGAPTPSIDRMADEGMTFFTFYAQPSCTPGRAAMQTGRIPNRS
                     GMTTVAFQGQGGGLPAAEWTLASVLKTAGYQTFFTGKWHLGEADYALPNAQGYDEMKY
                     AGLYHLNAYTYGDPTWFPAMPEELRAMFNKVTKGSMSGNAGQKAVEEFKVNGQYVNTP
                     VVDGKEGVVGIPFYDEYVEKAAMDYLDRNGKAAKPFFMSINFMKVHQPNMPAPEFIGK
                     SMAKSKFADSVVENDTRIGRIMDKVRALGLDKNTYVFWTTDNGAWQDVYPDAGYTPFR
                     GTKGTDREGGNRVPAIAWGPGIKAGSKNYDILGGLDFMATFAKLGGATLPVKDREGQP
                     IIFDSIDFSPVLFGTGKSARETWFYFTENELLPGAVRYHQFKFEFNLRGDNGQATGGL
                     AVDTNLGWKGPEKYVAVVPQVFDLWQDPQERYDIFMTNFTETTWMGVVMGQELNKLAK
                     TYVEYPPRKLQSEGYTGPLTISGYQKFEFLREQLKKEGINIPLPTGN"
     gene            complement(3052..4044)
                     /locus_tag="VEJ00_02040"
     CDS             complement(3052..4044)
                     /locus_tag="VEJ00_02040"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXW99965.1"
                     /translation="MSSRTLVGLVLLLAIATAGPPIDAQQPTRVSKIGFLFTGDQVRT
                     APQIAAFRQELRELGYVEGKTVRLELRFAEGAAERVPQLARELVALKPDVIVATTDPA
                     IAAVRRETQTIPIVMVFSSDPAGTGFVASLARPGGNVTGLSTQSPELAGKRLELLREV
                     VPGLARVALLWNPDSRGNLLEYKETEVVARSLHVELQSVELSRAEDLERAFSAVTNGR
                     AQALIVSPGNPVAIARRDQVASFAQRSRLPSIYGVREDVDDDGGLMSYGPSRTDMYRR
                     AAIFVDKILKGAKPADLAVEQPTKFELVVNLRTAKALGLTISPSILRRADDVIR"
     gene            complement(4041..4205)
                     /locus_tag="VEJ00_02045"
     CDS             complement(4041..4205)
                     /locus_tag="VEJ00_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99966.1"
                     /translation="MAAIVSADAAGYFHLMVHDESRTLGALKAHRRELIDPKHVDRAT
                     YRVNHAPGIS"
BASE COUNT          875 a         1379 c         1353 g          921 t
ORIGIN      
        1 tgccatcaag ttcgactaca aaccgtccac gctcaagata atcaacaacg gccataccat
       61 ccagatcaac tatgacccag gtagcaccat tacggtcggc ggaaagcagt acgagctcgt
      121 gcagttccat ttccacaggc cgagcgagga gaagatcgac ggcaagtcat tcgatatggt
      181 ggcgcacctg gtgcacaagg acagcagcgg gaatcttgcg gtggtcgctg tgctggtccg
      241 ttccggccag tcaaattccc tgatcgcaac gctgtggagc catctgccgc aagagaaggg
      301 cgtggaacag ctcgtcgcga acacccagat cgatgcggcg aagctgctcc cttccgatcg
      361 agcgtactac acgtttaccg ggtcactcac gacgcctccc tgcagcgagg atgtgacttg
      421 gtttgtcctc cgcgacccga tgcagttctc caaacaagag gtcgcacgct ttgcgaagct
      481 ttacccgatg aacgcccgtc cggtgcagcc tttgaatgga cggctggttg aaacgagccc
      541 gtaggtgccc gctctcggaa gcggacatgg gcaccgaatt cataggctac aacttgctgc
      601 catccattgc ccgtttagac gacatagggc cgactgcttg aaaccggtcg gagctcggcg
      661 tacgctaaaa cggttcggag ggcggactcg atgtcagtgt gacctgtggg attgccgctt
      721 cgttgtagcc aggagggggt agtctatcga tgacattcac ttcaggtgga cctcaaagcc
      781 ggtgatcgga aacggcacac cgttgcggtg acgcaacgaa atgaagccac agcgatgtgg
      841 cgatgctgcg aagccccgtg ttgatcgcgg ggctttgcat tactacctct gggttgcacc
      901 cctcgtcaga caccgtccgg gtatcgatca acaggtgatc gagcaattca acccgtttcg
      961 gtccccccgc cttagtcaca atgtgcgcta tcgggtcgat tcgcgaccgt cgctgatcgg
     1021 ccaaatcccg tcataacgaa atgcgctcga tacctgacat tcgcaggtcc cggctgcaca
     1081 gaaagaacgg gcgatccctt cggaccgccc atcgtttctc gctcgaagat cagttccccg
     1141 tgggcagcgg aatattgatg ccctccttct tgagctgctc ccgtagaaac tcgaacttct
     1201 ggtagcccga gatcgtgagc ggacccgtgt agccttcact ctgcagcttg cgtggcgggt
     1261 attcgacgta ggtcttcgcc agcttgttca gctcctggcc catgacgaca cccatccacg
     1321 tcgtctcggt aaagttggtc atgaaaatgt cgtagcgttc ctgggggtcc tgccagaggt
     1381 cgaaaacctg cgggacgacg gcgacatact tttccggacc cttccatccc agattggtgt
     1441 ccaccgccag gccgcctgta gcctgcccgt tgtcgccacg caaattgaac tcgaacttga
     1501 actggtgata gcgaacggcg ccgggcaata gctcgttctc ggtgaagtag aaccaggttt
     1561 cccgggctga cttgccggtg ccgaacagca ccggcgagaa gtcgatacta tcgaagatga
     1621 tcggctgacc ttcgcggtcc ttcacgggca gcgtcgcccc gccaagcttc gcgaacgtgg
     1681 ccatgaaatc gaggcccccc agaatgtcgt aattcttcga gccggccttg atcccaggac
     1741 cccaggcaat cgccggaacg cgattgccgc cttcccgatc agtgcctttg gtgccgcgga
     1801 acggcgtata gcctgcatcc gggtagacgt cctgccacgc gccattgtcc gtggtccaga
     1861 agacataggt attcttgtcg agaccgagcg cgcggacctt gtccatgatt ctgccgatcc
     1921 gcgtatcgtt ctccacgacc gagtcggcga atttgctttt ggccatcgat ttgccgatga
     1981 actccggggc gggcatgttc ggctggtgca ctttcatgaa gttaatgctc atgaagaacg
     2041 gcttggcggc cttgccgttg cggtcgagat aatccatggc tgccttctcg acgtactcgt
     2101 cgtagaacgg gatgccaacc acgccttctt tgccgtcgac caccggcgta ttcacgtatt
     2161 gaccgttgac cttgaactcc tcgactgcct tctgacccgc attgccggac atggaaccct
     2221 tggttacttt gttgaacatc gcccgcagct cttcgggcat ggcgggaaac caggtcgggt
     2281 cgccgtaggt gtacgcgttg aggtggtaca ggcccgcgta cttcatctcg tcgtagcctt
     2341 gggcgttcgg cagcgcatag tccgcctcac caaggtgcca tttgccggtg aagaaggtct
     2401 gatagccagc cgtcttgagc accgacgcca gtgtccattc agccgcgggt aggccgccgc
     2461 cctggccctg gaacgccacg gtggtcatgc cgctgcgatt gggtatgcgg ccggtctgca
     2521 tcgcggcgcg gccaggggta cagctcggct gggcgtagaa cgtgaagaaa gtcatgcctt
     2581 catccgccat gcggtcgatg ctgggtgtcg gtgcaccgcg gttcgggcct ccaccgtaga
     2641 caccggcgtc tccgtaaccg aagtcatcgg acacgatcag gatgatgttg ggtttctgga
     2701 ccggtgcctg cgcgagccca gcgcatggta ttgcagccgc caacgctacc gcaaaccctg
     2761 cgagcaccat tgaaaatgat cgcggaatcc tagtcatagg gtggtctcct tgtggataaa
     2821 cacggccgga aaccctagcg aggcgccatg cgaccagcgc ggttaggggc accaatcatt
     2881 cactcaaata cacgccttgt aaactcgctg gaaatctgcc ggcgtgatgc tgtcaaaact
     2941 gaaggattgc tccacagtgc catgttgcga acgtctggtt cttgagatgc cgaaagaccg
     3001 cagcgggtcg gctgccgacc gtcgctgacc ggccacaaac ggaccttgcg gctaccgaat
     3061 cacgtcgtcc gcgcggcgga ggattgacgg cgaaatggtg agaccgagcg ctttggcggt
     3121 cctgaggttg accaccagct cgaacttggt cggctgttct accgcaagat ctgcgggctt
     3181 ggcgcctttc aggatcttgt cgacgaagat ggccgcgcgc cggtacatgt cggtcctgct
     3241 gggcccgtag gacatgaggc cgccatcgtc gtccacgtcc tccctcactc cgtaaatcga
     3301 tgggagccga ctccgctgcg cgaagcttgc gacttgatca cgtctcgcaa tggcgacggg
     3361 attccccgga gacacgatca gcgcctgcgc acgcccgttc gtgaccgctg agaaggcgcg
     3421 ctcgagatcc tccgcacgag acagctcgac ggactgaagt tccacgtgca gcgagcgggc
     3481 caccacctcg gtttctttgt attcgagcag gtttccccgg gagtcgggat tccagaggag
     3541 cgcaacgcgc gcaagcccgg ggacgacctc ccgcagcagc tccagcctct tcccggctag
     3601 ttccggagac tgggtgctga gcccggtgac gtttcccccc gggcgcgcga gactcgccac
     3661 gaatccggtg ccggcagggt ccgagctgaa taccatcacg atcgggatcg tctgtgtctc
     3721 ccgtctgacc gccgcaatgg cgggatcggt ggtcgccacg atcacgtccg gcttgagggc
     3781 taccagctcc cgggcgagtt ggggaactcg ttcggctgcg ccctcggcga agcggagctc
     3841 gaggcgcacc gttttccctt cgacgtaacc gagctcccgc agctcctgcc tgaacgcggc
     3901 gatctgaggc gcggtcctca cctgatcgcc cgtgaagaga aacccgatct tcgagacccg
     3961 ggttggctgt tgggcgtcga tcgggggacc ggccgtggca atggcgagca gaagcacgag
     4021 gccgacgagc gttcgcgagc tcatgaaatg cctggagcgt ggttgacgcg gtacgttgcg
     4081 cggtcgacgt gcttcgggtc gatcagctcg cgccgatgag ccttcagcgc gccaagtgtc
     4141 cgactctcat cgtgcaccat gagatggaaa taacccgccg catcggctga gacgatcgcg
     4201 gccaagcgac gctggtcccg taccctccac tcccctcggc agacgttggg tgtgagcgga
     4261 ctatagtggc gacaccttta ctccgcaact acgactcgat gtccgttatg gggtcgcgag
     4321 ttgcccgttg aacccgaaac tgcgtagaca gatagcggtc gtcggcgcca cgcattgaac
     4381 cggacgaagc tggcgatcgt tgcaaagtcg gatctccttc aaccgaaagg agatcacgat
     4441 ggaatctccc cgtggtactc gactgtcgag aacggtctgg acttcagtca tcacttgaac
     4501 tggtagaact gctcgttcag gtattgct
//
LOCUS       DASPUL010000089         5807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_14248, whole genome shotgun sequence.
ACCESSION   DASPUL010000089 DASPUL010000000
VERSION     DASPUL010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14248"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1121
                     /locus_tag="VEJ00_02050"
     CDS             213..1121
                     /locus_tag="VEJ00_02050"
                     /inference="COORDINATES: protein motif:HMM:NF025185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HXW99967.1"
                     /translation="MSTASQSPTMATGRSARWPGWFPFLLLPAAVIFFRNRMPAWLFM
                     WSLSFAIFAALKWMTWWTAREQAIPRTGCSIAYLLLWPGMDAESFLDSSLHPERPRLR
                     EWAWAAVKTALGVVLIWVLARRIPAGQPLLRGWDGLFGLIFLLHFGSFHLIAMFWQAM
                     GVDAEPIMSKPILSKTLSEFWGTRWNLGFRQLAHEFVFRPLCKRIGVASAGMAVFVVS
                     GLIHDLVISVPARGGYGLPTAYFVLQGLGVTLERSNLGAKLGLQAGPRARVFTVALTA
                     LPAFWLFHPPFVLRVIIPFMHAIHAL"
     gene            1250..1570
                     /locus_tag="VEJ00_02055"
     CDS             1250..1570
                     /locus_tag="VEJ00_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99968.1"
                     /translation="MSFNRKLLWVQSGFTVLTIIAFGTLTLALHAELLRGDRAAMGLA
                     CFIGCYWTARILVDAFYFSHEDWPKGRQFVIGHILLTSLFVALAASYWSLFVWQVWLR
                     AKSQ"
     gene            complement(1584..2726)
                     /locus_tag="VEJ00_02060"
     CDS             complement(1584..2726)
                     /locus_tag="VEJ00_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXW99969.1"
                     /translation="MDFQLNDEQLHLKKSVREFAEREIKPHVMKWDEAGDFPLCAIKE
                     IGKLGLLGSIFPPEYGGAGMGYVEYVSIIEELSRVDGSVGIIVAAHTSLCSNHIFLAG
                     NESQKKKYVSKLATGEFIGAWGLTEPASGSDAGSARMTAVRRGNNWVLNGTKTFCTNG
                     HYADVIVVLAVTDRTAHTHGLSAFLVEKDTKGFRPGKKENKLGLRASDTAELIFEDCV
                     IPAENLLGAEGQGFPDAMRVLDGGRISIAALSLGMAEGAYECALKYSKQRKQFGKAIS
                     DFQAIQWKLADMATEIDAALLLTMRAASMKDAGFKTTQESSMAKLYASEVAVRCANES
                     VQIFGGYGFTKDYPAEKFYRDVKLCTIGEGTSEIQRLVIARQLLRD"
     gene            complement(2978..4168)
                     /gene="hemW"
                     /locus_tag="VEJ00_02065"
     CDS             complement(2978..4168)
                     /gene="hemW"
                     /locus_tag="VEJ00_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HXW99970.1"
                     /translation="MPLGIYISVPFCRTKCSYCNFASDVFSRTVFGRYGDRVCADIAA
                     APETARGMGGTIEEKVDSIYLGGGTPTVLDAAQLKRIFDAVRGQFRVLPDAEITIECA
                     PDTLAPNVLDALLRCGVNRVSLGVQSFVDGEAAAVGRLHKRVTVVEDIVLLRAAGIAN
                     INIDLIAGLPHQTNESWQVSLREGIATGVPHLSVYMLEVDEDSRLGRELIAGGTRYHA
                     HFVPDENATADFYLTACEALDAAGVAQYEISNFARPGFKSRHNLKYWTRQPYLGFGVD
                     AHSMLACRSPQAEAVRFATADVLEKYIAGAPLQKTLVSPGMALEESFFLGLRLNRGVD
                     LSEITARFGDESLVNVRPAIAELISDELVEEQRGHIRLTSRGRLLSNEVFQRFLTEDI
                     LNSK"
     gene            4305..>5807
                     /locus_tag="VEJ00_02070"
     CDS             4305..>5807
                     /locus_tag="VEJ00_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HXW99971.1"
                     /translation="MAQEEEVLGKAYDSRLMRRLLTYLRPYRWQVTIAIASIVLKSFG
                     DVLGPYLIKVAVDRYIAPVPSAASGLWGWLSPRPLVGIAQISTIYLSLLVLTFLLEFL
                     QTYFMQWTGQKVMFDLRSQIFRHLQRMHVAFYDKNPVGRLVTRVTTDVDALNDMFTSG
                     VVSIFDDIFTMLGIMGIMLCMNWKLALIAFAVLPLIILATKLFRDRVRDSYRRIRTAI
                     ARINAYLQEHVSGMVVLQLFNRERKAYNRFSEINRSHMDAFKDAIMAYAVYYPVVDFF
                     SAIAIACVIWYGGQDIIRNIVSTSVAVDFSRHTLWPFHWVATAASLGVLIAFTQYAQR
                     FFRPIMDFSEKYNILQSAMAASERIFKLLDTPVDVVSPAAVKKPEGAGRIEFDHVWFA
                     YREVAEEGKNRVATGASPVQPEQSPAELETDHGGAAARSDGYGPTPDWVLRDVTFTIE
                     PGETVAVVGHTGAGKTTMISLLLRFYDVQKGAVRIDGVDVKDMDLADLRSR"
BASE COUNT         1093 a         1745 c         1691 g         1278 t
ORIGIN      
        1 cgtggttctc gaccgtcgag atggcacggg ccagagcgcg cgcatcgccc gcacgcacag
       61 actcgatcac ggactgaagg tcgtgattca agctgccagt gtaaagcagc ggctaacaaa
      121 ctgaaactgc cgaaacttcc accgccgccg aattaccagc gtttattgtg cgcacacggg
      181 ccgcgcgata cagtgaaatc aggagacatg gcgtgagcac ggcttcacag agcccgacga
      241 tggcgaccgg gagaagcgcg cgttggcctg gatggtttcc ctttctgctc ctgcctgcgg
      301 cagtaatttt ctttcgcaac cgcatgccgg cctggttatt catgtggagt ttgtcgtttg
      361 caattttcgc cgccttgaaa tggatgacct ggtggacggc gcgcgagcag gcaattccta
      421 gaaccgggtg ctcgattgcc tatctcttgc tctggccagg catggatgcg gagagcttcc
      481 tcgattcaag cctacacccg gaacggccgc gacttcggga atgggcttgg gcagcggtaa
      541 agacagctct gggagttgtg ctgatctggg tcctggctcg gcgaattccc gccgggcaac
      601 ccttgctgcg agggtgggac ggactcttcg gcctgatttt cctgctgcac tttggcagct
      661 tccacttgat cgccatgttc tggcaggcaa tgggagtaga cgccgaaccg atcatgtcaa
      721 aaccgatcct ctcgaagaca ctgagtgagt tctggggaac gcgttggaat ctggggttcc
      781 gccagctcgc gcacgagttc gtctttcggc cgctgtgcaa gcgcattggc gtggccagtg
      841 ctgggatggc agtcttcgta gtctctggcc tgatccatga tctggtcatt tcggttccag
      901 cgcgtggcgg ttatggcctc cccacggctt atttcgttct tcaggggctg ggagtcacgc
      961 tggagcgctc caatctcgga gcgaagctgg gactgcaggc aggcccccgg gcgcgggttt
     1021 ttaccgtcgc gctcaccgct cttccggcct tctggctctt tcatccgccc tttgtgctac
     1081 gtgtcatcat ccctttcatg cacgctatcc acgccctatg acgcattctc ttactggacg
     1141 ctgctttgat cttgttctct ggctggcggg agctgggcac ttcgtgattc tggcggccag
     1201 ttttcaggtc ccgtttcgct tacggtggaa gcaagacttg gcgcagctga tgtctttcaa
     1261 ccgcaagctg ctgtgggtgc agagcggctt cacggttctg acgatcattg cattcggcac
     1321 cctcacttta gcgctgcacg cggaattgtt gcgcggagat cgcgctgcga tgggcctggc
     1381 ttgttttatc ggatgttatt ggacggcacg catcctggtg gacgcgttct atttttccca
     1441 cgaggattgg ccgaaaggaa ggcagtttgt gatcggccac attctgctga cgagcctgtt
     1501 cgtggctttg gccgcgagtt attggagcct gttcgtgtgg caggtttggc tgagagccaa
     1561 aagccaatag ccaacggcgg gtgttagtcc ctcagcaact gtctcgcaat caccaggcgc
     1621 tggatttcgc tggtgccttc gccaattgtg catagcttca cgtcccggta aaacttctcg
     1681 gcgggatagt ctttggtgaa gccgtatccg ccgaagatct gcacactctc gttggcacac
     1741 cgcaccgcga cctcgctggc ataaagcttg gccatagacg attcctgcgt agtcttgaac
     1801 ccggcgtctt tcatcgaggc ggcgcgcatg gtcagcagca aagcggcgtc gatttcagtg
     1861 gccatgtcgg cgagcttcca ctggatcgcc tgaaagtcgc taatggcctt gccgaactgc
     1921 ttgcgctgct tcgaatattt cagtgcgcac tcgtaggcgc cttcggccat gcccagggac
     1981 aacgctgcaa tcgagatgcg gccgccatcg agtacgcgca tggcgtcggg gaagccctga
     2041 ccctcggcac cgagcaggtt ttccgcagga attacacagt cttcgaagat cagttcggcc
     2101 gtgtcactgg ccctcagtcc gagcttgttc tctttcttgc cggggcgaaa tcccttggtg
     2161 tctttttcca ccaggaacgc cgacaagccg tgcgtgtgcg cggtgcgatc ggtcacggcg
     2221 agcaccacaa tcacatcagc gtaatgcccg tttgtgcaaa aggtcttggt gccgttcagc
     2281 acccagttgt tccccctgcg cacggcggtc atgcgcgcgc tgcccgcatc ggagccagac
     2341 gctggttccg tcagccccca ggcgccgata aactcgccgg tggccaactt gctgacgtat
     2401 ttcttcttct gggattcatt gccggcgagg aaaatgtggt tcgagcacag cgaggtgtgg
     2461 gcggcgacga tgatccccac cgagccgtcg acgcgcgaca gttcttcgat gatcgagacg
     2521 tactccacgt agcccatgcc cgcgccgcca tattccggcg ggaagatcga gcccaacagg
     2581 ccgagcttgc cgatctcctt gatcgcgcac agcggaaaat cgccggcttc gtcccatttc
     2641 atgacatgcg gcttgatctc gcgctcggca aattcgcgga cactcttctt caggtgcaac
     2701 tgttcgtcgt tcagctggaa atccaaggtt cctccggcag gaaggcgctt ttctctggta
     2761 caagttccgt tacgcccgaa ttttgattag aacatagcgc cggacaggga agaagttacc
     2821 gccggcgggg aaaggaaagg gtggaaacaa ccgcatgaac actggcgttt ctgcgtgtcg
     2881 tcgtcggcca cccaaccgac cggatggttt gggaatgagc accgctacgc tcttcgtgag
     2941 ccagcgctga cgatccgtga aaaattatac gctcgtttca cttcgagttg agaatgtcct
     3001 cggtgaggaa tcgctggaat acttcgttcg aaagcaatcg cccgcgcgaa gtcaggcgaa
     3061 tatggccgcg ttgctcctcg accaattcat ctgagatcaa ttccgcgatc gcagggcgca
     3121 cgttcaccag cgactcgtca ccgaaacgcg ctgtgatttc agacaaatcg actccccggt
     3181 tcaaacgtaa tcccaggaag aagctttcct caagagccat ccctggcgag accagggtct
     3241 tctgcaaagg agccccggca atatatttct cgaggacatc tgcggttgcg aaacgcaccg
     3301 cttccgcctg cggggagcgg cacgccagca tcgagtgcgc gtctacgccg aagccgagat
     3361 aaggctgtcg cgtccaatac ttcaggttgt gacgtgattt gaagccaggg cgcgcgaaat
     3421 tggaaatctc gtactgcgcg acgccggctg cgtccagtgc ttcgcaggca gtgagataga
     3481 aatcagccgt ggcgttctca tcgggtacga agtgagcgtg atagcgtgtc cctcccgcga
     3541 tcaattccct gcccagccgc gaatcttcgt cgacctccaa catgtagacg ctgaggtgag
     3601 gcactccggt cgcgatacct tcccggagcg acacttgcca gctttcattt gtctggtgcg
     3661 gcaagccggc gatcaaatcg atattgatgt tggcgattcc cgctgcccgc agcaggacaa
     3721 tgtcttcaac cacagtcaca cgcttatgca gacgcccgac cgccgcggcc tcaccgtcga
     3781 cgaaggactg cactcccaaa ctgacgcggt tcaccccaca gcgcagcagc gcgtccaaca
     3841 cgttcggagc tagcgtgtcc ggagcgcatt caatggtaat ttccgcgtct ggcagaacgc
     3901 ggaactggcc gcgcacagcg tcgaagattc tcttgagttg agcagcgtcg agcacggttg
     3961 gcgtgccgcc acccaggtag atcgagtcaa ccttttcctc gatcgtacct cccattcccc
     4021 tcgcagtctc gggtgcagcc gcgatgtcgg cgcacacgcg atccccatag cgcccgaaga
     4081 ccgtgcgcga gaacacgtcg gacgcgaagt tgcagtagct gcacttcgtc cggcaaaagg
     4141 ggacggaaat gtatatcccg agcggcattg agccattctc ccgaagtaat tatcccacca
     4201 aaaccttttt catcctggcg gctgctatgg ccatctattt gaggtaccct gtttgggagc
     4261 gcctgcagcg aggcggtatc cgacggggcg tattttcgta acccatggcc caggaagagg
     4321 aggtattagg caaagcctac gacagccggc tcatgcgccg gttgctcacc tacctgcgtc
     4381 cctatcgctg gcaggtgacg attgcgattg cctcgatcgt cctcaaatcg ttcggtgatg
     4441 tgctcgggcc ttatctgatc aaggttgcgg tcgaccgcta catcgccccc gttccgtcgg
     4501 ccgcctcggg cttatggggt tggctgagcc cgcggccgct ggtgggtatc gcacagattt
     4561 ccaccatcta tctcagcctg ctggtgctga ccttcctgct tgagttcctg caaacctatt
     4621 tcatgcagtg gaccggccag aaggtcatgt ttgatctgcg cagccagatt ttccgccacc
     4681 tgcagcgcat gcacgttgcc ttttatgaca agaacccggt cggacgcctg gtgacgcgtg
     4741 tcaccaccga cgtcgatgcg ctgaacgata tgtttacctc gggcgtcgtc tcgatcttcg
     4801 atgacatctt cacgatgctc gggattatgg gcatcatgct gtgcatgaac tggaagctgg
     4861 cgctgatcgc gttcgccgtg ctgcccctga tcattctggc gaccaagctg ttccgcgacc
     4921 gggtgcggga ttcctatcgc cgcattcgca ccgccatcgc ccgcatcaac gcctacctgc
     4981 aggaacatgt cagcggcatg gtggtgctcc agctattcaa ccgcgagcgc aaggcctaca
     5041 accgcttctc cgaaatcaat cgcagccaca tggacgcatt caaggacgcg atcatggcct
     5101 acgcggtcta ctatcccgtg gtcgacttct tctcggcgat cgcgattgcc tgcgtcatct
     5161 ggtacggcgg ccaggacatt attcgtaaca tcgtctccac gagcgttgcc gttgacttca
     5221 gccggcacac gctctggcct ttccactggg tcgctacggc ggcatcattg ggcgtgctga
     5281 tcgcgttcac ccagtatgcg cagcgcttct tccgcccaat catggacttc agcgagaagt
     5341 acaacatcct gcagtcggcg atggcggcca gcgaacgcat cttcaagctc ctggacactc
     5401 cggtagacgt tgtctcgccg gcagcggtga aaaaacctga gggcgcaggc cgcatcgaat
     5461 tcgatcacgt ctggttcgcg tatcgggagg tggcggagga gggcaagaat cgtgtggcga
     5521 cgggcgcctc gcccgtccag ccagagcaaa gcccggctga gctagagaca gaccatggag
     5581 gggcggccgc ccggagcgat ggctacgggc ctacacccga ctgggtgctt cgcgacgtca
     5641 ctttcaccat cgaacccgga gagaccgtcg ccgtggtcgg ccatacgggc gcgggcaaga
     5701 ccacgatgat ttcgctgctg ctgcgctttt atgacgtcca gaagggcgcg gtccgcatcg
     5761 acggtgtcga cgtcaaagac atggacctcg ccgacctgcg cagccgc
//
LOCUS       DASPUL010000090        11324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_425378, whole genome shotgun sequence.
ACCESSION   DASPUL010000090 DASPUL010000000
VERSION     DASPUL010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 11324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11324)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11324
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425378"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VEJ00_02075"
     CDS             complement(<1..455)
                     /locus_tag="VEJ00_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99972.1"
                     /translation="MSRHQPGFTRDLIEVILGYGLIVGVIWMPDLPQRIASPIVLIFT
                     LAVVVARWPTRNELGLGGRGLVPSLWILPTAIVLAGVSMLVARRLGTLHPLFNGDLQH
                     ITGYILWTLYQQFLLNDYFLPRLIRLIGNENIAVGLTGVLFALAHLPNLA"
     gene            complement(517..1413)
                     /locus_tag="VEJ00_02080"
     CDS             complement(517..1413)
                     /locus_tag="VEJ00_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXW99973.1"
                     /translation="MALVEIRNLTKVFAHGASALGASVRTARDVRAVDDVSLDIHSGE
                     TLGLVGESGSGKSTLGRLILRLIEPTAGSIRFEGHDLLSAGKNEMRRLRRDLQIIFQD
                     PFASLDPRFRVEDVIAEPLIIHGREGATNERSARVHDREGRDFTRAETSAEELSASAA
                     EGKAARRRRVVELLCAVGLDESALRRYPHEFSGGQRQRIGIARALALRPKFIVCDEPV
                     SALDVSVGAQIVNLLAQLQRDFGLTYLFISHSMPVVRYLSTRVAVMYRGKIVEIGDAA
                     QITESPQHAYTRSLLEATPEMV"
     gene            complement(1649..2557)
                     /locus_tag="VEJ00_02085"
     CDS             complement(1649..2557)
                     /locus_tag="VEJ00_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXW99974.1"
                     /translation="MVQGFTVLLSSDVLSPTQFAPAVYSVAAVCLWGAADFAGGYGSR
                     RANAFVLTAFSHVCAFALMFGIALAQPGAFPSRTSILWAVVAGAVGGFSLAIFYRALS
                     LGQMGLTAPIAALIGAAIPTLADIAMEGAPSHWTVAGFGLAIVAIWLITRPEPSDGEA
                     MAKDDTGRPAGVGMAALAGVGFAGFYLCIHQASGSPIWIAAISRIGSFTSTAIAVAVT
                     RAPLAMDRPRAALGMLAGCLDISASALFIFASQRGRLDEAVVITSLYPAMTVLLARLL
                     LKEHFSRWRFVGLLAALAAVPLIAAG"
     gene            2815..5742
                     /locus_tag="VEJ00_02090"
     CDS             2815..5742
                     /locus_tag="VEJ00_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXW99975.1"
                     /translation="MIGQTISHYRIVEKLGGGGMGVVYKAEDTRLHRFVALKFLPEDV
                     TRDPTALSRFQREAQAASALNHPNICTIHDIGEADGQAFIVMEYLDGETLKHVINNRP
                     LDLETLLNVAIDVTDALDAAHAKGIVHRDIKPANLFLTSRGAAKILDFGLAKLETAAE
                     PSDATQTKTDAHRLTSPGSTVGTVAYMSPEQARGKPLDARTDLFSFGAVMYEMATGTL
                     PFRGDTTALLFESILHKAPVAPVRLNPDLPPELERVINKALEKDRDLRYQHASDMRAD
                     LKRLKREVDSSKSSTAATEVEPAAVVAGSSSTVKKESSGKAAPASSGAAAAPVAPRSS
                     KTALWVAVASVLVVVVAAGYYLTHRPAAKLTEKDTIVVADFANSTGDPVFDDALKQAL
                     SVSLRQSPFLNVLSDDKVASTLKLMTKPVNTPLTPDVARELCQRADSKAYVAGSIANL
                     GSQFVVGLKAVNCLNGDVLAQEQSSASGKEKVLDALGEAASKLRSQLGESLASVQKFD
                     APLGQETTPSLEALKAQALARKTEMEKGSAAALPFFQRAIDLDPNFATAIEGVGIMYG
                     NVGQATRGGEYLTKAFALRDRASEREKLHITSMYYMSVTGELDKAVETFREWEENYPR
                     DDIPYNNLSTVYSAQGKVEQGLEQALASSRLNPDNVITAENVMGFLVTLQRYDEAQQI
                     YAQTLARGMDDDALHQVRYGLAFIQRDSKTMAEQVAWYDTRPQLQNEILGLQSDTEAY
                     AGHEAKAREMTRAATESAVRTDNKESAALWLVTAAWREALVGNFEEARRDVEKSLELA
                     PDSRDARVLGALTLARAADVAHARSMAQKVGQQYQAHTLVQSYWLPLIDAQSSLSTGQ
                     AQNALDQMQRIVNLDFSLAFSSANSSCAYPNYLRGEAFLAAGQSSAAAAEFQKIIDHP
                     GLVWNCVTGALAHLELGRADALAGDKAKARAAYQDFFNLWKDADPDIPVLKEAKAEFT
                     KLQ"
     gene            5914..6588
                     /gene="rpe"
                     /locus_tag="VEJ00_02095"
     CDS             5914..6588
                     /gene="rpe"
                     /locus_tag="VEJ00_02095"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXW99976.1"
                     /translation="MIELAPSILAADFARLGEQVRAAGEGGGSVIHVDIMDGHFVPNL
                     TIGPPVVTSLRKATDLPLDCHLMIENPDEFIPAFAEAGADWISVHQEAVRHLDRTLHL
                     IKSHDCLAGVVINPATPVDTLSEVLEIVDYVLVMSVNPGFGAQKFIPSTLHKMRALAE
                     IRSHRGLNYRIEVDGGVALDTVADVVRAGAEILVAGNAVFGHGDPRKNAQELLKAATE
                     AAMQKV"
     gene            6688..8166
                     /gene="bamD"
                     /locus_tag="VEJ00_02100"
     CDS             6688..8166
                     /gene="bamD"
                     /locus_tag="VEJ00_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HXW99977.1"
                     /translation="MSRRISLIVVLALLLALTAACTNKKSQNPLANLGSKQPDKVLFD
                     KAMDAMHHNRFDVARMTLQVLINTYPDSEFIARAKLAVGDSWYAEGGTAALAQAEQEY
                     DDFETFFPNMPEASEAQLKIANIHFQQMEKADRDYTHAKRAEEEYRKMIMTYPDSKLV
                     PEAKQRLLEVQEVLAEREFDIGRFYYLRDSYPASMARLESLIERYPLYSRADEALYLL
                     GQNYEAQIARLRQTPTCDAHGMPKGCTLEKYKAEYSEELTKGAAAAYDKILTRYPVMD
                     RSDDAKKRLVALHQPVPRPTRAAVAQNKAEEASRTETTGLKKLLAVVKKGPDTSKAAK
                     VGEPALVDPTPVAAPEIVRNEALAVVNASRSDLGLKIVNKSGPSAGTETAPSQGETAS
                     GGSPFGQGGAATPAQAATPDPNELKPNVADSNELKPLDTSVDPTLPPPPQVNEIGQGQ
                     GTAALASSSSAAVSADNSLASDKEISSSKKKKKKGLKKLNPF"
     gene            8360..10558
                     /locus_tag="VEJ00_02105"
     CDS             8360..10558
                     /locus_tag="VEJ00_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXW99978.1"
                     /translation="MARKILLADDSVTAQNMGRKILTDAGYEVITVNNGSAALKRISE
                     SKPDLIVLDVYMPGYSGLEVCQRLKETHETARIPVLLTVGKLEPFKPEEAKRARAEAF
                     IVKPFEASELLSTLTKLEDKIVPRAEGSKPGRFARANAALDEGRYDKTVAIDEDSGWK
                     DRIAFPSKKKKEKEREKAVEADDGDDPALYNAVNRDLRTTVERHGDQRPQDTKANQDR
                     AEEARVDLGALAPEGLPKDVTAEEIAALAAAAAQVKAKFGDDVEGKIEESAPQPAVAA
                     GETNAEAATGEKVEVKTDSKQEPQAQEVAASEAAVATFAEQKSESGQKAEEEKPEEKV
                     ETPVAARAEAEVEAVGLAAPDTASPSEPAPAFSTTNAGDEAGSQRLAAQPAGEPFTMA
                     AAAGAGNGASSSRWTAVAVALAPEEAAISLELEMQKAHAAFAAAESAKTGSVVIIPEP
                     ATAPEPPVVESATVTTSAVAELAAEPGAASQAVPEHAASDGGSYSPEAIAQVREFESV
                     ASSRSAEAPAEAAESPFTPAQFVEPAIPSAPERETFVEEIPAAQRVAAEPEIAQEATQ
                     EPPQREQETPQPAEKQEAIEEARPEEPMRAEEVVAAASEVVAGVSASTQAGPEATGHA
                     EKPETDVAETTTAAAWATWRRIRESGDSTGQSHAAQGNGGTDQSGASAPSQAEAATAM
                     AAAAGAEGPAQDAAQDSDSPEIASIVDSVLADLRPKIVAEISKKLGKKKQ"
     gene            10849..>11324
                     /locus_tag="VEJ00_02110"
     CDS             10849..>11324
                     /locus_tag="VEJ00_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXW99979.1"
                     /translation="MPHDLPKSYEPGAIEARWADYWVKERLFSVPTPPEGETRPVFTL
                     LLPPPNVTGRLHMGHMLNQTQMDIVVRWHRMRGFITLWLPGTDHAGIATQMMVEKQLE
                     KEGKKRRDLGREKFIERVWDWKQHYGGAILDQMKRLGASVDWDREYFTMDENLSHAV"
BASE COUNT         2401 a         3397 c         3651 g         1875 t
ORIGIN      
        1 gcaagattcg ggaggtgagc cagagcgaaa aggacgcccg ttaaacctac ggcgatgttt
       61 tcgttcccaa tcagacggat gagccgcggc aagaaataat cgttcagcag gaactgctgg
      121 tagagcgtcc acaggatgta acccgtgatg tgctgaaggt cgccgttgaa cagcggatgc
      181 agcgttccga gccgccgggc aaccagcatg ctcacgccgg cgagaacgat cgcggtgggt
      241 aggatccaga gagaagggac caggccgcgg ccgcccaggc ccagctcgtt ccgtgtgggc
      301 cagcgcgcga ccactaccgc cagcgtgaag atcagcacaa tcggagacgc gatgcgctgg
      361 gggaggtcgg gcatccagat gacgcctacg atcagcccgt aaccgagaat cacctcgatc
      421 aagtcacgcg taaaaccggg ctggtggcgg gacattgggc cagtatggca caggcgccgc
      481 ctcttcgagc gcgctccgct gctgcgagga gacgtcttag accatttccg gagttgcttc
      541 gagaaggctg cgggtatagg cgtgctgcgg gctttcggtg atctgggccg cgtcgccgat
      601 ctcgacgatt tttccgcgat acatgacggc gacgcgcgtt gagagatagc ggaccaccgg
      661 catggagtgc gaaatgaaaa gataggtcag gccgaagtcg cgctgcagtt gcgcgagcag
      721 gttgacgatc tgggccccca cgctgacgtc gagcgcagag acgggctcgt cgcagacgat
      781 gaatttcgga cgcaacgcga gcgcccgggc gatgccgatg cgctggcgtt gccctccaga
      841 gaattcgtga gggtagcggc gcagggcgga ttcatcgagg ccgacggcgc acagaagttc
      901 gacgacgcgc cttcggcgag cagcctttcc ctcagcggct gaagccgaga gttcttctgc
      961 ggaggtttcg gcgcgagtga agtcgcgccc ttcccggtca tgcacgcgag cagaccgctc
     1021 attagtagca ccctctcgcc cgtggatgat cagcggctcg gcgatcacgt cttccacccg
     1081 gaatcttggg tcgagcgatg cgaagggatc ctggaagatg atttgcaggt cgcgccgcag
     1141 gcggcgcatc tcatttttcc cggcggagag caggtcgtga ccttcgaagc ggatgctccc
     1201 cgcagtgggc tcgatcaggc ggagaatcag ccttcccagc gtgcttttcc ccgagccgga
     1261 ttcgccgacg aggccaagcg tttcgccgga atggatgtcg agcgagacgt cgtcgaccgc
     1321 gcggacgtct cttgccgtcc ggactgaggc cccaagtgca gatgcgccgt gggcaaagac
     1381 cttcgtcagg ttgcggattt cgacgagcgc cacggtgcga tgctaacagc acggagtacc
     1441 gagcgcgagt ggcttgctgg gcggttggtt tgaatcctgc atgggggctt ttcgatggcg
     1501 tcatcccgag cggagccgtt tttcaggcgg agcgagtgac ctcccccgcg gtaccactct
     1561 ggggagatcc ctcggcccgc tggtgaaagc gcgggccttc gggatgacgc cacgggaacg
     1621 tttagaactg ggtaaccgcc cagatccgct aacccgccgc aatcagcgga accgctgcca
     1681 gggccgccag caagccgacg aaccgccagc ggctgaagtg ttctttcagc aggaggcgcg
     1741 ccagcaagac ggtcatggcg ggatagagag aagtgatcac gacggcttcg tcgagccgtc
     1801 cccgctggct ggcgaagata aacaaagcgc tggcggagat gtcgaggcag ccggccagca
     1861 tgccgagggc ggctcggggc cgatccatcg ccagcggcgc gcgcgttacg gcaaccgcga
     1921 tagcggttga agtgaaggac ccgatgcggg agatggccgc gatccagata ggcgagccgc
     1981 tcgcctggtg gatgcagagg tagaagccgg cgaaccccac tccggccagg gcggccatgc
     2041 ccacgccggc gggccggccg gtgtcatctt tcgccattgc ctcgccgtcg gacggttcgg
     2101 gccgcgtaat cagccagata gcgacgatgg ccagcccgaa accggcaacg gtccagtggc
     2161 tcggcgcgcc ttccatggcg atgtcggcca acgtcgggat cgccgccccg atcagtgcag
     2221 cgatcggtgc cgttaagccc atttgtccca gggacagggc gcgatagaag atggccagcg
     2281 agaagccgcc taccgcgccg gcgaccaccg cccagaggat gctcgtgcgc gacgggaaag
     2341 cgccgggttg cgccaacgca atgccgaaca tgagcgcaaa ggcgcagacg tgcgagaacg
     2401 cggtcagcac aaacgcgttg gcgcggcggg agccgtagcc tccggcgaag tcggccgcgc
     2461 cccagagaca caccgcggca acgctataga ccgccggggc aaattgagtc ggtgagagca
     2521 cgtccgacga gagtagcacg gtgaagccct gcaccacgtg cagcggttcg ccgttatccg
     2581 gaagcttaat gccggaagag gatgcaatcc gaattcagtt tttggggcgg agtcatcccg
     2641 agggcccgcg ttttcaccag agggccgagg gatctcgcgc ggagccatag cgcagcccca
     2701 attgactctc agccgtgccg cctctaacat gggggccaat ttgcaagctg cgccgcgaac
     2761 cggtttgatc ggcgatggtc gcgccgccac ttcgcaaacc gggttcactc ccatatgatc
     2821 ggccagacca tctctcacta ccggattgtg gagaagttgg gcggcggtgg catgggagtg
     2881 gtgtacaagg ccgaagacac gcgcctgcat cgtttcgtgg cgctcaagtt tctccccgaa
     2941 gacgtgactc gcgatcccac ggcgctgagc cgctttcagc gcgaagccca ggccgcatcg
     3001 gcactgaacc atcccaatat ctgcacgatc cacgacatcg gcgaagcgga cgggcaagcg
     3061 ttcatcgtga tggagtacct cgatggcgag acgctcaaac acgtcatcaa caatcgcccg
     3121 ctcgatctgg aaactctgct caacgtcgcg attgatgtga ccgatgcgct cgacgccgcg
     3181 cacgccaagg gcattgtgca tcgcgatatc aagccggcca atttgtttct gaccagccgg
     3241 ggtgcggcga agattctgga cttcggcctg gccaagctgg agaccgccgc tgagccgagc
     3301 gacgccacgc aaaccaagac cgatgcccat cgcctgacaa gcccgggatc gaccgtaggc
     3361 actgtggctt atatgtctcc ggagcaggcg cggggaaagc cgctcgatgc tcgcactgat
     3421 cttttttcgt tcggggccgt gatgtacgaa atggccaccg gcacgttgcc gtttcgcggg
     3481 gatacgacgg cgctgctgtt cgagtcgatc ctgcacaagg ctccggtcgc ccccgtgcgg
     3541 ctcaatcccg acctgccgcc ggagctggag agggtcatca acaaggctct tgaaaaggac
     3601 cgcgacctgc gctaccagca tgcttccgac atgcgtgcgg acctgaagcg gctgaagcgg
     3661 gaagttgact cgagcaagag ctccaccgca gccacagaag tcgagcctgc cgccgttgtg
     3721 gcgggttcga gctccaccgt aaaaaaggaa tcgagcggga aagcggcccc agcctcctcc
     3781 ggtgctgcag cggcccccgt cgcgccacgg tcctcgaaaa ctgcgctctg ggttgctgtc
     3841 gcaagtgtgc tggttgtagt ggtggctgcc ggctactacc tcacacatcg gcctgcagcc
     3901 aagctcacgg agaaagacac gattgtagtc gcggacttcg ccaattccac cggcgacccc
     3961 gtcttcgacg acgcgctaaa gcaggcgctg agtgtgtcgc tgcggcagtc gcccttcctg
     4021 aacgtgctct ccgacgacaa ggtcgcatcc acgctgaagc tgatgaccaa gccggtcaac
     4081 acgcccctga ctcctgacgt cgcgcgcgag ttgtgccagc gcgccgacag caaggcctat
     4141 gtcgcgggct cgatcgccaa tctgggcagc cagtttgtgg ttgggctgaa ggccgtaaat
     4201 tgcctcaacg gcgacgttct ggcccaggag caatcgtcgg cttcgggcaa agagaaggtg
     4261 ctggatgccc tgggcgaggc tgccagcaag ctgcgcagtc agttgggtga atctctcgcc
     4321 tcagtgcaga agttcgatgc tccactgggt caggaaacga ctccctcgct ggaggcgctg
     4381 aaagcccagg ctcttgcccg gaaaacggag atggaaaaag gctcggctgc cgccctgccg
     4441 ttctttcaac gcgccattga cctcgatccg aactttgcca cggccataga aggcgttgga
     4501 atcatgtatg gcaacgtcgg ccaggcaacg cgcggaggtg agtacctgac caaggcattc
     4561 gcgcttcggg accgcgccag cgagcgagag aaactgcaca tcacttcgat gtactacatg
     4621 tctgtcacgg gggaactgga caaggcggtc gagaccttcc gggaatggga ggagaactat
     4681 ccccgcgacg acattccata caacaacctg tccaccgtct attctgcgca ggggaaggtg
     4741 gagcaggggt tggagcaggc gctggcctcg tcacggctga atcccgacaa cgtgatcacg
     4801 gccgagaacg tgatgggctt cctagtcacc cttcagcgct acgacgaggc ccagcagatt
     4861 tacgcccaga cgctggcgcg cggcatggac gatgacgctc tgcaccaggt cagatacggc
     4921 ctggccttca tccaaaggga cagcaaaacg atggccgagc aggtggcgtg gtacgacacg
     4981 cgccctcaat tgcaaaacga gattctggga ttgcagtccg acactgaagc ctatgcggga
     5041 cacgaggcga aggcgcgcga gatgacgcgg gcagcaaccg aatccgcggt gcggaccgat
     5101 aacaaggaat ccgccgcttt atggctggtc acggccgcct ggcgagaggc gctcgtggga
     5161 aatttcgagg aagcgcgccg cgatgtcgag aaaagccttg aactggcgcc ggacagtcgc
     5221 gatgcgcggg ttttgggagc tctgactctg gcgcgcgcgg cagacgtggc ccacgcccgg
     5281 tcgatggcac aaaaagtcgg ccagcaatac caggcgcaca cactggtaca gtcgtattgg
     5341 ctgcctttga ttgatgctca gtcctcgtta tcaaccgggc aagcccagaa cgcgctcgat
     5401 caaatgcaga ggatcgtcaa tctggacttt tccctggctt tttcctctgc aaacagctcc
     5461 tgcgcctatc caaactatct gcggggtgaa gcatttctcg ccgccggaca aagcagcgcc
     5521 gctgcggctg aattccagaa gatcatcgac catcccggcc tggtctggaa ttgcgtcacg
     5581 ggcgcgctgg cgcaccttga actgggccgg gcagacgcgc tggcggggga caaagccaaa
     5641 gctcgagccg cttatcagga tttcttcaat ctgtggaagg atgccgaccc ggacattccc
     5701 gtcctgaagg aagccaaagc ggaattcacg aagctgcaat aaagctgcaa ttcaaagaat
     5761 acgcggtcgg gcagcaggtg gcccctcaag ccgcctttgg cttgagtggg gtagaggctt
     5821 ttctgcgtgt tagaatttcc gcagaagctt tctcggcaag agcacgattt ctgaggagac
     5881 cggttcccag ggcctggttt ctgagagcta cctttgatcg aactggcacc gtcgattctt
     5941 gcggctgatt tcgcacggct tggcgagcag gtgcgcgcgg ccggcgaagg gggcgggagt
     6001 gtcattcatg tggacatcat ggacggacac ttcgtgccga accttacgat cgggccgccg
     6061 gtcgtcacct cgctgcgcaa ggcgaccgat ctgccgctcg attgccacct gatgattgaa
     6121 aatcccgacg agttcatccc ggcgtttgcc gaggcgggag cggattggat ttcggtgcat
     6181 caggaggcgg tccggcacct ggatcggacg ttgcatttga tcaagagcca cgactgcctg
     6241 gccggggtgg tcatcaaccc ggcaacgccg gtggacacgc tctcggaagt gctggagatt
     6301 gtggactatg tgctggtgat gtcggtaaat ccgggctttg gagcgcagaa gttcattccg
     6361 tccaccttgc acaaaatgcg agctttagcc gagattcgga gccaccgcgg gctgaactac
     6421 cgcatcgaag tcgatggcgg cgtggcgctg gacactgttg cagatgtggt ccgcgcgggc
     6481 gctgaaattc tggtggcggg caacgccgtg ttcggccacg gcgatccaag aaagaatgcg
     6541 caggaactgc tgaaagcggc gaccgaggcc gcaatgcaga aagtctaatc ggatttgcga
     6601 ttgccgattg ccgatttgtg actgcgagtc gagaggtttt cgatcggcaa tcgaaaatcg
     6661 gcaatcgaaa atgtcccccg aggtcttatg tcacgtcgaa tttcgctgat cgtcgtcctt
     6721 gcccttttgc tggcccttac agccgcctgc accaacaaga aatcgcagaa cccgctggcc
     6781 aacctggggt caaagcagcc ggataaagtc ttgtttgaca aggccatgga cgccatgcac
     6841 cacaaccgct tcgatgtggc ccgcatgacc ctgcaggtgc tgatcaatac ctatcctgac
     6901 tcggaattca tcgcccgcgc caagctggcg gttggcgatt cctggtacgc cgagggtgga
     6961 accgccgctc tggcccaggc ggagcaggaa tacgacgact tcgaaacctt cttccccaac
     7021 atgccggaag cgtccgaggc gcagctgaag atcgccaaca tccacttcca gcagatggaa
     7081 aaggcagacc gggactacac gcacgccaag cgcgcggaag aagaataccg caagatgatc
     7141 atgacttatc ccgacagcaa gctggtgccc gaggccaagc agcggctgct tgaagtgcag
     7201 gaagtgctgg ccgagcggga atttgatatc gggcggttct actacctgcg ggattcgtat
     7261 ccagcgtcga tggcgcgcct ggaatcgctg atcgaaaggt atccgctata cagccgcgcc
     7321 gacgaggccc tctatcttct gggccagaac tacgaagccc agattgcgcg gttgcggcag
     7381 accccgacgt gcgacgccca cggtatgcct aaaggttgca ccctcgaaaa atataaggcc
     7441 gaatacagcg aggagttgac caagggcgcg gcggcggcct atgacaagat cctgacgcgc
     7501 tatccggtga tggatcgctc cgatgatgcc aagaagcggc tggtcgcact gcaccagccg
     7561 gttccgcgtc ccacccgagc tgcggttgca cagaacaagg ctgaggaggc gagccggacc
     7621 gagaccacgg gtctcaagaa gcttctggcg gtcgtaaaaa aaggaccgga cacttccaag
     7681 gcagcgaagg tcggcgagcc tgcgctggtc gatcctacgc cggttgcagc gcctgaaatc
     7741 gtgcgcaatg aagccctggc ggtcgtcaac gcgagccgga gcgatctggg cctcaagatc
     7801 gtgaacaagt caggcccgtc cgccggaacg gaaaccgccc ccagtcaagg ggagacagcg
     7861 tcgggagggt cgcccttcgg gcagggcgga gccgccacgc ccgcgcaggc ggcaaccccg
     7921 gatccgaacg aactgaagcc gaacgtggcg gattccaacg agttgaaacc actggatacc
     7981 agtgtggatc ccacgctgcc accgccgccg caagtgaatg agatcggtca aggccagggg
     8041 accgctgcgt tggcctcgtc ttcttcggct gccgtgagcg ccgacaacag ccttgccagc
     8101 gacaaggaaa tctccagcag caagaaaaag aaaaagaagg gcttgaagaa gctgaatccg
     8161 ttctaggaga agcgtttagc atagtgctca ccccgggcga cccgaaagcc cggggcggaa
     8221 gtgtcgttcg acgcctgtct ccccggtctc actccggtag gtgctgacca ctagtaacca
     8281 gttacttccc ttagggatca ttgacttacg ttgagacgca ggttaccttc gcggtatctc
     8341 ccctcatcag aggatatcct tggcccggaa gatactgctt gccgatgaca gcgtgaccgc
     8401 ccagaacatg gggcgcaaaa tccttacgga tgcgggttac gaggtcatca cggtcaacaa
     8461 cgggtccgcc gcgctgaagc ggatcagcga gagcaagccc gacctcatcg tgctcgacgt
     8521 gtacatgccc ggctatagcg ggctggaggt gtgccagcgc ctcaaggaaa cgcatgagac
     8581 cgcacgcatt ccggtcctgc tgacggtggg aaagctggag cctttcaagc cggaagaggc
     8641 caaacgcgcc cgcgccgaag ctttcatcgt aaagcccttt gaagccagcg agctgctgag
     8701 caccctgacc aagctggaag acaagatcgt gcctcgtgcg gaaggctcca agccgggccg
     8761 ctttgcccgc gccaatgccg cgctggacga aggccgctac gacaagacgg tcgccatcga
     8821 cgaggattcg ggctggaagg atcgcattgc cttccccagc aagaaaaaga aggaaaagga
     8881 gagggaaaag gcggttgagg cggacgacgg cgacgatccc gcgctttaca acgcggtgaa
     8941 ccgcgacctg cgcacgaccg tcgagcgcca cggggatcag cggccgcagg acacaaaagc
     9001 aaaccaggac agggcggagg aagcgcgcgt cgatctgggg gcgctggcgc cggaagggct
     9061 gccgaaggat gtaaccgcgg aagagattgc agcactggcg gcagctgccg cgcaagtcaa
     9121 ggccaagttc ggcgacgacg tcgaaggcaa aatcgaggaa tcagcaccgc agccggctgt
     9181 ggccgccggg gaaacaaacg cggaagcggc gaccggcgaa aaggtcgaag tcaagacgga
     9241 ctcaaagcaa gaaccgcagg cccaggaagt ggcagcctcc gaagcggccg tggcgacctt
     9301 cgccgagcag aaatccgaaa gcgggcaaaa ggccgaagaa gagaagccgg aagagaaggt
     9361 tgagacgcca gtggcggcgc gggctgaggc tgaggtggaa gcagttggtc tcgcggcccc
     9421 cgacacagct tcgccatctg agcccgcacc tgccttctcc accaccaatg cgggtgatga
     9481 agcggggtct cagagattag cggcgcagcc ggctggtgaa ccgttcacca tggcggccgc
     9541 tgccggggct ggaaacggcg cttcctcttc gcgctggacg gcggtggccg tggctctcgc
     9601 gcccgaagaa gctgcgattt cgctcgaact cgagatgcag aaggcccatg cggcattcgc
     9661 cgcggcagag tccgcgaaga cggggtcggt ggtgatcatc ccggagccag cgacagcgcc
     9721 tgagccaccg gtggtcgaat ccgccacggt aacaacttcc gcggtcgcag aactggcagc
     9781 ggaaccaggc gcggcgtcgc aggcggttcc ggagcacgct gcgagcgacg gcggcagcta
     9841 ctcgccagaa gcgatcgccc aggtgaggga attcgagagc gtagcttcat ctcgctccgc
     9901 ggaagcacct gcagaggcgg ctgaatcgcc tttcactcct gcacagtttg tcgagcctgc
     9961 aatcccatct gcgccggaga gggagacctt cgtggaggag attcctgccg cgcaaagagt
    10021 cgcggcggaa ccggaaatcg cgcaggaagc cacgcaggag ccgccgcaaa gagagcagga
    10081 aacgccacaa ccggcagaaa agcaggaagc gatcgaagaa gcgagaccag aagagccgat
    10141 gcgggcggaa gaagtcgttg cggcggcgag cgaagttgtc gctggtgtga gcgcatccac
    10201 ccaagccggc cccgaagcca ccgggcatgc ggagaagccg gaaaccgatg tggctgaaac
    10261 cactactgcc gctgcctggg cgacctggcg gcgcattcgc gagtcgggcg actctacagg
    10321 ccaatcccac gccgcacaag gcaatggcgg gacggaccag agcggcgcgt cggctccctc
    10381 ccaggccgaa gctgcaactg ccatggccgc cgccgccggc gccgagggac ccgcgcagga
    10441 tgcagcgcag gattccgatt ctcccgagat cgccagcatc gtcgacagcg tgctggccga
    10501 cctgcgtcca aagattgtcg ccgaaatctc caagaagctg ggcaagaaga agcagtaacc
    10561 gctcagactt agcttgaacg ttcttcctaa cgtcgctgtg gcacgccttt cgcacggtac
    10621 aacttcaact ggactttaat caagggtcga gaggccgcga gaaaatggcc gccttgccct
    10681 tatcgatcgg ggaccgcact tcgtatccca atgaggcgcc gtttgaccag tcttttcttg
    10741 cgcctgtaac atcagaattt ggcactcagc aaacgggtat tcgagcttgg cgtgcggaac
    10801 tgatcgctgc ctccggacga ctgcggtttg ctgaatgttg attgcgccat gcctcacgac
    10861 cttcccaaat cctatgagcc cggcgcgatt gaagcgcgct gggccgacta ctgggtcaaa
    10921 gaacggctgt tctccgtgcc cacgccgccc gagggcgaaa cgcggccggt gttcaccctg
    10981 cttctgcctc cgcccaacgt gaccggacgc ctgcacatgg ggcacatgct caaccagacg
    11041 cagatggaca ttgtcgtgcg ctggcaccgc atgcgcggct tcattacgct gtggctgccg
    11101 ggcaccgacc acgccggcat cgccacgcag atgatggtcg agaagcaact cgagaaggaa
    11161 ggcaagaagc gtcgcgacct gggccgggag aaattcatcg agcgcgtgtg ggactggaag
    11221 cagcactatg gcggcgccat cctcgaccag atgaagcgcc tgggcgcctc ggtcgactgg
    11281 gaccgcgagt acttcaccat ggacgagaac ctctcgcacg cggt
//
LOCUS       DASPUL010000091         3686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_15075, whole genome shotgun sequence.
ACCESSION   DASPUL010000091 DASPUL010000000
VERSION     DASPUL010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15075"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            445..1932
                     /locus_tag="VEJ00_02115"
     CDS             445..1932
                     /locus_tag="VEJ00_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXW99980.1"
                     /translation="MGIAVSPQIHPQVAEFIDKPRKMLINGKWVNSASGKTFPTYNPA
                     TGEVLAQIAEGDRDDIEQAVRAARKAFDGGPWRRMTPSERGRLLWKLADLLEQHTEEF
                     AYIESLDNGKPLSIAKVADVPLSVDMFRYMAGWATKIEGNTIPISAGKYFAYTLREPI
                     GVVGQIIPWNFPLLMAAWKLGPALATGCTIVLKPAEQTPLSALRLGELVMEAGFPEGV
                     VNIIPGYGETAGAALVAHPDVDKVAFTGSTEVGKLIVHAAAGNLKKVTLELGGKSPNV
                     VFKDADLNTAIPGAASAIFFNHGQCCCAGSRLYVEKAVYDQVVEGVAKEAEKITVGSG
                     LDPQTQMGPLVSEEQLKRVCGYLEAGFSEGAKAMVGGHKAGDRGYFVEPTVLVDTKED
                     MKVVREEIFGPVVAAMPFTDPEEIIPRANNSEYGLAAAVWTRDISKAHRMAEQLRAGT
                     VWINCYNVFDAALPFGGYKQSGWGREMGHEVLNNYTQTKAVCAKI"
     gene            complement(2221..2790)
                     /gene="pyrR"
                     /locus_tag="VEJ00_02120"
     CDS             complement(2221..2790)
                     /gene="pyrR"
                     /locus_tag="VEJ00_02120"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HXW99981.1"
                     /translation="MNAKNSVRKTGLRQKAQLMSASEIERTLVRLAYEIVEKNGGVDA
                     LGLVGIRRRGVPLAERLGKIITRIENAKVPVGTLDITFYRDDLSTLGPKPVVQEKDVG
                     FSIEDKNIVLVDDVLFTGRTTRAALDALFSHGRPRRVQLCVLIDRGHREIPVEATFVG
                     RNVQTTMNEVIEVKLTEIDEQEKVLLMEK"
     gene            complement(2894..>3686)
                     /locus_tag="VEJ00_02125"
     CDS             complement(2894..>3686)
                     /locus_tag="VEJ00_02125"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXW99982.1"
                     /translation="KDELESKEHSLRWHSLELQRKNLELQEISFTDVLTGVWNRRYLE
                     EILAAEAGQVLRNYQRARGGDIRKMDHRDLVFIMVDVDFFKEVNDLHGHPAGDRLLQL
                     VAERLSTVVRKSDVLVRWGGEEFLIMSRSTDPAGTPAFCSRVLEVMSAEPFDLGYGIS
                     VRKTCSVGWAAYPWCRGAYEALCAEESIALADAALYRAKSLGRNQGVGFVPKDGASMA
                     PEAINLSAVQVPNSALARIIQTECPVSFRMPEAEAETQPVDDRRV"
BASE COUNT          706 a         1055 c         1200 g          725 t
ORIGIN      
        1 aggtttggac gaacgaaggg cgggtcatcg actggtttga tgccaaacag ggcgaggggc
       61 gctggtttct ggagcatgcc acgcaggtga agaatatctg ggtaccctac ggggagtagg
      121 gtttcgggca tcgccaagca tcagcgcagg ggctaaagcc ccgggaattt ccgctccgta
      181 acgcggcgct aaagcgccgc tcttccacac tattgccgac tgccgtgcct tcatgcagcc
      241 gctgaaaagc cgctcttcca cggcaggcgt caacttcaga acaaaacctc caggtcgatt
      301 tctcttggcg gacagccgag ggtgcctgtc cctccatacc aggcgtttgc atttgtttgc
      361 ccacagcgct agcatttgtc ccggcgtttt ccttcgtctg ggattcttct cttctgcaag
      421 cgcgtgactg accaaggagg tcctatgggc atcgcagttt ccccgcaaat ccatccgcaa
      481 gtggcggagt ttatcgacaa accacgcaag atgttgatca acggaaagtg ggtgaattcc
      541 gcttccggca agacttttcc tacgtacaat ccggcgacgg gcgaggtgct ggcccagatc
      601 gcggaaggcg atcgcgacga tatcgaacag gccgtgcgcg cggcgcgcaa ggcatttgac
      661 ggcggcccgt ggcgccgcat gacgccgtcg gagcgcggac ggctgctgtg gaagctggcc
      721 gatttgctcg agcagcacac ggaagaattt gcctacatcg agagcctcga caacggcaag
      781 ccgctaagca tcgccaaggt ggccgacgtg ccgctctcgg tcgatatgtt ccgctacatg
      841 gcgggttggg ccacaaagat tgaaggcaat acgattccga tttcggccgg caagtatttc
      901 gcatacacgc tgcgcgagcc cattggagtt gtggggcaga tcatcccgtg gaacttcccg
      961 ctgctgatgg cggcgtggaa gctaggtccg gcgctggcca cgggctgcac gattgtgctg
     1021 aaaccggcgg agcaaacgcc gctctcggcg ctgcgcctgg gtgaactggt gatggaagcc
     1081 gggttccccg agggcgtggt caacatcatc cccggatacg gcgagacggc aggggcggct
     1141 ttggtggcgc atccggacgt ggataaggtc gcgtttaccg gatcgaccga agtgggcaag
     1201 ctgatcgtgc atgctgcggc gggcaacctg aagaaagtca cgctggaact gggcggaaag
     1261 tcgccgaacg tagtgttcaa agatgccgat ctcaacactg ccatccccgg ggcggcgagc
     1321 gcgatcttct tcaaccacgg gcagtgctgc tgcgccgggt cgcgcctcta cgtcgagaag
     1381 gcggtttacg accaggttgt ggaaggagtc gccaaggaag cggagaagat cacggtgggc
     1441 tccgggcttg atcctcaaac ccagatgggg ccgctagttt ctgaagagca gctgaagcgc
     1501 gtctgcggat acctggaagc gggattctcc gagggggcga aggctatggt cggcgggcac
     1561 aaggccggcg accgcggtta cttcgttgaa ccgaccgtgc tggtggacac gaaggaagac
     1621 atgaaggtcg tgagggagga aatcttcggg ccggtggtgg cggcgatgcc gttcaccgat
     1681 cctgaggaga tcattccgcg cgccaacaac agcgagtacg ggctggcggc ggccgtgtgg
     1741 acgcgcgaca tcagcaaggc gcatcgcatg gcggagcagc tgcgcgcggg cacggtctgg
     1801 attaactgct acaacgtctt cgacgcggcg ctgccgttcg gaggctataa gcagtccggc
     1861 tggggccgcg agatgggaca cgaagtgctc aacaactaca cgcagaccaa ggcggtgtgc
     1921 gcgaagatct aggctcctgg ttacaggcga ggacggcggc gcgagccgcc gttctttttt
     1981 cgcgcgcaga ttcgcagggc ggtgtgggaa gaccggtgcc gtccctggtg ggactccggc
     2041 cttgccctca tcctttcccg gctcctacgt gcagggctat tacataccgc cccttcgggg
     2101 ctgttgtcag gtggcggttg caccagtggt ttctatcaga aactttcagt tgctcacaga
     2161 acgccgtgcg ccaacgcgcg gatcttaccg agtgggtcca gctttctgac tagaggttct
     2221 ttacttttcc atcagcagaa ctttttcctg ctcgtcgatc tcggtgagct tcacctcgat
     2281 cacttcattc atcgtggtct gcacgttacg acccacgaag gtggcttcga ccgggatctc
     2341 gcggtggcca cggtcgatca gcacgcagag ctgcacgcgg cgggggcggc catgggagaa
     2401 cagcgcgtcg agggcggcgc gggttgtgcg tccggtgaac aggacgtcgt cgacgagcac
     2461 gatgttcttg tcctcgatcg aaaacccaac gtctttctcc tgcaccactg gcttggggcc
     2521 gagtgtcgaa aggtcgtcac ggtaaaaggt gatgtcgaga gttcctacgg gaaccttggc
     2581 gttctcgata cgggtgatga tcttgcccag gcgctcggcg agcggtacgc cgcggcggcg
     2641 aataccgacg aggcccaggg catcgacgcc gccatttttt tccacgattt cataggccag
     2701 gcgcaccaag gtgcgctcga tttctgatgc ggacatgagt tgcgccttct ggcgaaggcc
     2761 agtcttgcgt acagaatttt ttgcattcat gagatgagga cgcctcgcga gccgaagccg
     2821 gcttctcact gtagtcaagg caactgcgga ggtcaagccg cggattgggc gcgcccctgg
     2881 ccagactgaa aacctatact cggcggtcgt cgacgggctg agtttcggct tctgcttctg
     2941 gcattctgaa gctgacggga cactcggttt ggatgatgcg ggcgagcgcg gagttgggta
     3001 cctgtacggc agaaaggttg atggcctccg gagccatgct cgctccgtct ttcgggacga
     3061 accccacgcc ctgattcctg cccagggact tggctcgata cagcgctgcg tcggcgagag
     3121 cgattgactc ttccgcgcac agggcctcat atgcgccacg gcaccaggga tacgcggccc
     3181 aacctacgga acacgtcttg cgcacgctga tgccgtagcc caggtcgaag ggctcggcgg
     3241 acatgacttc aagaacgcgg ctgcagaatg cgggcgtgcc ggcaggatcg gtggagcggc
     3301 tcatgatcag aaattcctcg ccaccccatc gcaccagcac gtcggacttg cgcaccaccg
     3361 tagagagcct ttccgcgacg agttgcagca ggcggtcgcc ggcgggatgg ccgtgcaggt
     3421 cgttcacttc cttgaagaag tcgacgtcga ccatgatgaa gacgagatca cggtggtcca
     3481 tcttgcggat atcgccaccg cgggcgcgct ggtagttgcg caggacctgt ccggcctcgg
     3541 ccgcgagaat ctcttcgagg tagcggcggt tccagacacc agtcagaacg tcggtaaagg
     3601 aaatctcctg cagttcgaga ttcttgcgct gcagctcgag actatgccaa cggagagagt
     3661 gctccttgct ctccagttcg tctttc
//
LOCUS       DASPUL010000092         6003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1932489, whole genome shotgun sequence.
ACCESSION   DASPUL010000092 DASPUL010000000
VERSION     DASPUL010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932489"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VEJ00_02130"
     CDS             complement(<1..452)
                     /locus_tag="VEJ00_02130"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HXW99983.1"
                     /translation="MRKQQLTMLVSLLVFGVAALAQTPSRLDRVNVVRGTDDIRVEIS
                     SHGAVTPTLKSLDSPARVVVDLPATVMATGQSRIAVGTAGVKGVRIGMDGQTPPTTRI
                     VVDLDQACAYELTPDAAGKLVLTLHGNAVASAAQAEVKPVALNAPADQP"
     gene            complement(541..1836)
                     /locus_tag="VEJ00_02135"
     CDS             complement(541..1836)
                     /locus_tag="VEJ00_02135"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HXW99984.1"
                     /translation="MKLTTGILAITITMAAGAGLVTKATAQNTDAIDNARSVAKSLQQ
                     KQTNDTNAALDAAGVAAPSKPAPGAPAPAVKPAVIPGSTPAAAPASAAKPATAAPKPA
                     AAAKPAAKAAPSSSQSNQLQGVKVAQGSSEVQIQIQSSAAVTPRVSKLSSPARVLVEL
                     PETAIATSQTKIPVNSAGVKGVRIGMDGKTPPTTSVVVDLEKACAYEMGSGPGGQLIL
                     TLHTDGVTQETATSSPAPAKTATPAKTAAAKPAAAQVKTVAATPAAIPAPKAAVAKAP
                     VAKPAAEAKSQAKAAPAAKSAAPAKAVAAAVDSGKSAEPAKPKQEEKKWAMNGKRDPF
                     FSPVVQQASGSGCSTGKKCLEIGNINLRGVVKSDSGFIAVVTNNLNKAYFLRENDPVF
                     NGYVVKITGDSVVFQETVQDKLGKPFTREVVKRIYTPAV"
     gene            complement(1845..2489)
                     /gene="pilO"
                     /locus_tag="VEJ00_02140"
     CDS             complement(1845..2489)
                     /gene="pilO"
                     /locus_tag="VEJ00_02140"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HXW99985.1"
                     /translation="MATNFGELSGIKQWGVVIAGGLVVTAALYFGVFKSQNEKNATAQ
                     HALEDKIRENNELESYRPKLKQIEQQLAELKQQLEIEARIVPDEKQIDQFMTMLDGEA
                     QKAGVELRRYTAKDVKQQQYYTEVPFEMELDGPYYSVLNFFDRVSKLERIVNISGVLV
                     GTTKAPTDAKAKHTYQYAPNESVIATFMATTYFSHDSQPSAAGAGAKPATPAAK"
     gene            complement(2500..3117)
                     /locus_tag="VEJ00_02145"
     CDS             complement(2500..3117)
                     /locus_tag="VEJ00_02145"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXW99986.1"
                     /translation="MIKINLLETAKGKNKRGGGGPSMPSMEMGDMGSPKLKVLIVVVV
                     AGLFNLGYWYRLDHQAKAIAAQMKVEEERNRELADVKARFLERQTQANNYKRRVDVID
                     GLRQSQTGPVTLLAMMGETVNNTEAVWLTKMDDTGPTVNLVGTALSTDAVANLIANLQ
                     KTGFFKTVEIKETYQDETIKDMQAFQFTLSCDKAAGDTQPGKGKS"
     gene            complement(3114..4169)
                     /gene="pilM"
                     /locus_tag="VEJ00_02150"
     CDS             complement(3114..4169)
                     /gene="pilM"
                     /locus_tag="VEJ00_02150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HXW99987.1"
                     /translation="MFGFGSKSIVGLDVGSSSIKAVELKKKGGQIEVAHIGLEPLSSD
                     IVVDSMIVDSGTVSSAISKLFLDNQIKTKAVATAVSGHSVIVKKISLPSMSDQELAET
                     IEKEAAQHIPFDLADVSLDYQILSDEVGSPQMDVLLVAVKKDKILNYTNVLSMAGRTP
                     AIVDIDALALQNCYEYNYQPAPGQVVALLNLGASVMNINIVKGTTPLFPRDVSVGGHQ
                     YTDSLQKELDLSFDDAEALKLGRKVGTVSEDAKQPILQQVTEIIVLEIQKTFDFFRAS
                     AAGEHIEKIYLAGGSSKVPGLVEALRQEFSMPVELFNPFQRVTPPTEGLGAELVEQNP
                     GQMAVAVGLALRSFESL"
     gene            complement(4212..4448)
                     /locus_tag="VEJ00_02155"
     CDS             complement(4212..4448)
                     /locus_tag="VEJ00_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXW99988.1"
                     /translation="MADSREVMNIRQASQYLGVSPDTLYKYVGEQKIPAFKLGNRWRF
                     KKSRLDQWMEEKSSQMEPAMKRKPKAAGKAAGAQ"
     gene            complement(4852..5694)
                     /locus_tag="VEJ00_02160"
     CDS             complement(4852..5694)
                     /locus_tag="VEJ00_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99989.1"
                     /translation="MSQRFAAVVATVALFLGVVVGGSLAWAQAPTLAEQLAAQYKLAK
                     LGSDSSGYSITEEGTLLAVQKGGIIGVPYKNTLTRTATFQDGTVHASDVSNNSAAQKV
                     GKLFCGLHKCPTTPDAANDERTTKLFKVGDKVYPTKIDVNLDKDQVVMAIVACDTCNK
                     TDPPTYNKANVVFQFAKGTLAKTAAGPVEDTIGQLLTISEDTGQQDQSGQQQGQAQGQ
                     GQGQSQQQQGGEPQTIQMGMTMDQVQGALGQPQKIVNLGPKQIYVYKDLKVTFFGGKV
                     VDVQ"
     gene            complement(5820..>6003)
                     /locus_tag="VEJ00_02165"
     CDS             complement(5820..>6003)
                     /locus_tag="VEJ00_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99990.1"
                     /translation="KQGTAGLPKAPDTSTTDGDVPAPPPDKSAEKNLQDQQSAADQAE
                     SQAKQEAGSGSGGGLD"
BASE COUNT          948 a         1958 c         1719 g         1378 t
ORIGIN      
        1 ggctggtcgg ccggcgcatt cagcgccacc ggcttcactt cagcctgggc agccgacgca
       61 actgcgtttc cgtgcagggt cagcaccagc ttgcccgccg catctggcgt caattcatag
      121 gcgcaggcct ggtcgaggtc cacgacaatg cgagtggtcg gaggcgtctg cccatccatg
      181 ccaatgcgca cgcccttcac cccggctgtc cctaccgcga tgcggctctg cccggtcgcc
      241 atgaccgtgg ccggcaggtc caccaccacg cgggcaggcg agtccaggct cttgagcgtg
      301 ggcgtcaccg cgccgtgcga gctgatctcg acccggatgt cgtccgtgcc ccggactacg
      361 ttgactcgat cgagccggct cggtgtctgg gccagtgccg ccaccccgaa gaccagcaac
      421 gataccaaca tggtgagttg ttgctttcgc atttcgcctc tccccaaccc gcgggccggg
      481 cccgcgtgtc cttcgtcaag cttgaattca gcgccccggt ttttccggtt cgccgtcccc
      541 ctaaactgcc ggcgtgtaga ttctcttgac cacttcccgc gtgaacggct tgcccagctt
      601 gtcttgcacc gtctcctgga acaccaccga atcacccgtg attttcacta cgtagccgtt
      661 gaataccgga tcgttttccc gcagaaaata cgccttgttc aggttgttgg tgacgaccgc
      721 gataaaaccg ctgtcgcttt tcaccacgcc ccgcaggttg atgtttccga tctccaggca
      781 cttcttgccg gtggagcaac cggaaccgct cgcctgctgc accacggggc tgaagaaggg
      841 atcgcgtttc ccgttcatcg cccacttttt ctcttcctgc ttgggcttgg cgggctctgc
      901 actcttgccg gaatcaaccg ccgcagctac tgccttggcc ggtgcagcag acttggctgc
      961 aggggcagcc ttggcttgcg atttcgcttc tgccgccggc ttcgccaccg gagccttcgc
     1021 aaccgccgcc ttgggcgcag gaattgcagc cggtgtagcc gctacggtct tgacctgcgc
     1081 tgctgccggc ttggccgcag ccgtcttggc cggtgtcgcc gtctttgccg gggccggaga
     1141 gctcgtcgct gtttcctggg ttacgccgtc ggtgtgcagg gtcaggatca gttgtccgcc
     1201 agggcccgaa cccatctcat aagcgcacgc cttctccaga tccaccacca cgctcgtggt
     1261 cggaggagtc ttgccgtcca tgccgatgcg gacgcccttc actccggcgc tgttcaccgg
     1321 aatcttcgtc tgggaagtcg caatcgcggt ctcaggcagc tccaccagca cgcgagccgg
     1381 cgaactcagt ttgctgactc tcggcgttac cgctgcgctc gactggatct ggatctgaac
     1441 ttcgctcgac ccttgcgcca ccttgacgcc ctggagctga ttgctctggc tggagctcgg
     1501 ggcagctttc gccgccggct tggccgcagc tgccggtttc ggcgccgccg tcgcgggctt
     1561 cgccgcagac gcgggagccg ccgccggcgt cgatcccgga atcaccgcgg gcttgacagc
     1621 cggagcggga gcgcccggcg caggcttgga cggagccgca actccagccg catccagcgc
     1681 cgcgttggtg tcgttggtct gcttctgctg cagcgatttc gccacgctgc gcgcgttgtc
     1741 gatggcgtcg gtattctggg ccgtcgcctt ggtaacaagg cccgcgcccg ccgccatcgt
     1801 gatagtgatt gccagaatgc ctgtcgtcag cttcatagtc atccctactt cgccgccggc
     1861 gtcgcgggtt tcgccccggc tccggccgct gaaggttgcg aatcgtggct gaagtacgtc
     1921 gtcgccatga acgtcgcgat cacactctcg ttgggcgcgt actgataggt atgtttggct
     1981 ttcgcatcgg tcggggcctt ggtcgtgccg accagcacgc cgctgatatt gacgatccgc
     2041 tccagcttgc tgacccgatc gaagaaattc agcaccgagt agtacggccc gtccagttcc
     2101 atttcaaacg gaacctcggt gtagtactgt tgctgtttca cgtccttggc ggtgtagcgc
     2161 cgcagctcca cgcccgcttt ctgcgcttcc ccgtcgagca tggtcatgaa ctggtcgatc
     2221 tgcttctcat cgggaacgat gcgcgcctcg atctccagct gctgtttcaa ctcggccagc
     2281 tgctgttcga tctgcttcag cttgggccgg taggattcga gctcgttgtt ctcccgaatc
     2341 ttgtcttcga gggcgtgctg cgcggtggcg ttcttctcgt tctggctctt gaacacgccg
     2401 aaatacagag ccgccgttac caccagtccg ccggcaatca ccactcccca ctgcttgatg
     2461 cccgacaatt cgccaaaatt cgtcgccata ggtccgcctt tacgactttc ccttcccggg
     2521 ttgggtatcg cccgcggctt tatcgcaact cagggtgaac tggaacgcct gcatgtcctt
     2581 gatcgtttcg tcctgatacg tctccttgat ttcgacggtc ttgaagaatc ccgtcttctg
     2641 caggttggcg atcaggttgg ccacggcgtc ggtcgacagg gccgtgccca ccaggttcac
     2701 ggtcgggccg gtgtcgtcca tcttggtcag ccacaccgcc tcggtgttgt tcaccgtttc
     2761 gcccatcatg gccagcagcg tgaccgggcc agtctggctc tgccgcagcc cgtcaatcac
     2821 atcgacccgg cgcttatagt tgttggcctg cgtctgccgc tccaggaagc gcgccttcac
     2881 gtcggccagt tcgcggttcc tctcttcctc gaccttcatc tgggccgcaa tggctttggc
     2941 ctgatgatcc agccggtacc agtagcccag gttgaacaat cccgcgacca ccacgacgat
     3001 cagaaccttc agcttgggcg accccatgtc ccccatttcc atggagggca tcgacggacc
     3061 gccgccgccg cgcttgtttt tccctttcgc tgtttcgagc agattaattt tgatcatagg
     3121 gattcaaagc tcctcagggc caagcccacc gcgactgcca tctggcccgg gttctgctcc
     3181 accagttccg cgcccagccc ttccgtcggc ggggtgaccc gctggaacgg gttgaacagc
     3241 tcgaccggca tggagaactc ctgccgcaac gcttccacca gacctggaac cttcgacgac
     3301 cctccggcca gataaatctt ctcgatgtgc tcgcccgccg cgctggcccg gaagaagtcg
     3361 aacgtcttct ggatttccag cacgatgatc tcggtcacct gctgcaggat gggctgcttg
     3421 gcgtcttcgc tcaccgtgcc caccttgcgg cccagcttga gcgcttccgc gtcgtcaaag
     3481 ctgaggtcca gttccttctg cagcgaatcg gtgtactgat gcccgcccac gctgacgtcg
     3541 cgcgggaaca gcggggtcgt gcccttcacg atgttgatgt tcatcacgct ggcgcccagg
     3601 ttcagcagcg ccacaacctg gccgggcgcg ggctggtagt tgtattcgta gcagttctgc
     3661 agcgccaacg catcgatgtc gacgatagcc ggggtgcgcc ccgccatcga gagcacgttg
     3721 gtgtaattca gaatcttgtc cttcttgacc gccaccagca ggacgtccat ctgcgggctg
     3781 ccgacttcat cagagagaat ctggtagtcg aggctcacgt cggccagatc gaacgggatg
     3841 tgctgggccg cttccttctc aatggtctcg gccagctcct ggtcgctcat cgagggcagt
     3901 gagatcttct tcacgatcac cgagtgaccg ctcaccgcgg tggccaccgc tttggtcttg
     3961 atctggttgt cgaggaacag cttcgagatc gcgcttgaaa ccgtgcccga gtccacgatc
     4021 atcgaatcga ccacgatgtc tgaggacagc ggctccagac cgatatgcgc cacttcaatc
     4081 tgtccgccct ttttcttcag ctccaccgcc ttgatgctcg aggagcctac atccaggcca
     4141 acgatcgact tcgatccgaa tccaaacatg tgcccacctt tgtagcgccg gcgtctcgcc
     4201 ggccaatttg actactgcgc tccggcagct ttgccggctg ccttgggttt ccgcttcatc
     4261 gccggttcca tctggctcga cttctcttcc atccactggt ccagccgcga tttcttgaag
     4321 cgccagcggt tgcccagctt gaaagcggga atcttctgct cccccacgta tttgtaaagc
     4381 gtgtccggac tcacgcccag gtattgcgac gcttggcgaa tgttcatcac ttctcgcgag
     4441 tcggccatag ttcctttgcg cccaccgcag aatcgatcgc ccgcccggtt tctccggccc
     4501 gcaggcattt ccttcaggag acagcctcgc cctctatatc ggctccgagg gctttcggtg
     4561 tcctgagcat atatcagccc ggaaaagccc cggctcgaag cgaatctccg cgttttatag
     4621 ggtttttggc gggtttcctg gaatggccgt agaccgcggc caaggggtgt gtctatatat
     4681 tgggtttttg cgaaattcag atacaacagg cagtgggcgc tatgcaactc gccccaagtg
     4741 accgcaggaa cggtaactga agtactagag acggtacgga ggtactatgc cgcccagcta
     4801 agcctcggtt ttgcaaggct tagcccgagc actggtagcc tgcgcacccg attactggac
     4861 gtccacaacc ttgccgccga agaaggtcac cttgagatct ttgtagacgt agatctgctt
     4921 ggggcccagg ttcacaattt tctgcggttg acccagtgcg ccttgcactt gatccatcgt
     4981 catgcccatc tggatggtct ggggttcgcc gccctgttgc tgctggcttt ggccttgccc
     5041 ttggccctga gcttgaccct gctgctggcc gctctgatcc tgttgcccgg tgtcttcgct
     5101 gatggtcaac agttgtccga tggtgtcttc caccgggccc gccgcggtct tcgccagagt
     5161 tcctttggca aactggaaga ccacgttggc cttgttgtag gtggggggat cggtcttgtt
     5221 gcaggtgtcg caggcgacga tggccatgac gacctgatct ttgtcgaggt tcacatcgat
     5281 cttggtcgga tataccttgt caccgacctt gaacagcttg gtggttcgct cgtcgttggc
     5341 ggcatcgggg gtggttggac atttgtggag cccgcaaaag agtttgccca ccttctgagc
     5401 cgcggagttg ttgctgacat ccgaggcatg gacggttcca tcctgaaacg tggccgtacg
     5461 cgtcagcgtg ttcttgtacg gcacgccgat gattccgccc ttttggacgg caagcagcgt
     5521 accctcttcg gtgatggaat atccgctgga atcggaaccc agtttggcca gcttgtattg
     5581 cgcggccagc tgttcggcca gcgtaggcgc ctgcgcccac gcgagggaac cgccaaccac
     5641 tactccgagg aagagtgcca ctgtggcaac gaccgctgcg aatctctgcg acatcgaaaa
     5701 gcctcctgcc atctttggag aagaacgttc agcctactaa tgtttcaaga tcctgaatgc
     5761 gtcagcggag cgccggcaag ccagcgctca ttaatcgttc gacgaactcc cttcagctct
     5821 caatccagcc caccgccgga accggatccc gcctcctgct tagcctggga ttcggcttga
     5881 tcggcagcgg attgctgatc ctgcagattc ttctctgcgc tcttgtccgg cggaggcgcg
     5941 ggcacgtcgc catccgtcgt gctggtgtcg ggcgccttgg gaagaccggc ggttccctgc
     6001 ttc
//
LOCUS       DASPUL010000093         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1932708, whole genome shotgun sequence.
ACCESSION   DASPUL010000093 DASPUL010000000
VERSION     DASPUL010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932708"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VEJ00_02170"
     CDS             <1..553
                     /locus_tag="VEJ00_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99991.1"
                     /translation="LVILGLCVLGVSASAQDIFKINYFANNGVSGAPDATVRIDNPGT
                     ASTENLCAMIYVFDNDQQLTECCGCLVTANGLRTLSVGKNLTNNPLTGVVSNNGVIKI
                     VSSAPNNSPCEPASGVEPTPTVRAWASHIQNKVGSAYPVTETEFSDAALSTQEFSFNL
                     EEQCFFAERLGSGHGICSCGTGD"
     gene            669..1709
                     /locus_tag="VEJ00_02175"
     CDS             669..1709
                     /locus_tag="VEJ00_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXW99992.1"
                     /translation="MKISAFILLFVFLACSFLSAQVTPASITAPSVIADVDGHRLTES
                     DLDRKMSGSLLQARYQYYLTERKALDKLVDDELIALAAEHQHMTTDQFLEKEVYKDIK
                     DPTEDQLQVYYEGMESNESYESLHDRLIEHIRDLRRAKARAAYVKTLRTQANLQILLA
                     PPVADVNVEGAELHGAKDAPVVLLEFADYECPYCQKVNPLLVKLREEFGDKVTIAYKD
                     FPLPMHRNAQKASEAARCAGEQGKFWEYHDLLYSTKALEVNDLKKHAQTLKLDEAQFN
                     KCLDSGAEAEIVKKNLEEGKTLGLSGTPSFFANNHFFSGGVDYNTLREMVEQQIRART
                     TPQSLTQTASTK"
     gene            1800..>2829
                     /locus_tag="VEJ00_02180"
     CDS             1800..>2829
                     /locus_tag="VEJ00_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXW99993.1"
                     /translation="MSRRQTKGRENTAHGKNSFGLLLAPVVFALLAVAVLAAPPLAGA
                     QAVTVTVSPGAAQVVTGSSLQLTSTVNGVAPGTQNQLVTWSLSGPGCSGLSCGLINAA
                     GQYTAPASAPNPPTVFATATSIFNPGASGSATLTIAAPVPVTVTVTPSTFSIKPGAQQ
                     QFSATVTGTSSNQVVWGASGAGCFGIQCGTIDFNGVYTAPTQVPNPAVVSVTATLLSD
                     QSKSGSAKVTIGSGIAVSVTPPTPQILLGQQQQFAATVTGSNNTAVNWTVSGAGCAGA
                     TCGNITAGGLYTAPAAMPGAPTVTITATSAADVNTNGSATLTLFATPSVSVQPHSVHV
                     NPGAKQVFT"
BASE COUNT          614 a          847 c          810 g          558 t
ORIGIN      
        1 cctggttatt ctggggcttt gcgtgctggg agtcagcgcg tcagctcagg atatcttcaa
       61 gatcaactac ttcgcgaaca acggcgtatc gggtgctccg gatgccacag ttcgcatcga
      121 caatccgggc acggccagca ccgagaacct gtgtgcgatg atctatgtgt tcgacaacga
      181 tcagcagttg accgagtgct gcggctgcct cgtcacggcc aacggtctgc gcaccctgtc
      241 ggtgggcaag aatctgacca acaacccgct gaccggcgtc gtttccaaca acggcgtgat
      301 caagatcgtg tcctcggccc cgaacaactc cccttgcgag ccagcgtcgg gtgttgaacc
      361 cacgcctaca gttcgcgcct gggcgagcca cattcagaac aaggtcggca gcgcgtatcc
      421 agtcactgaa accgagttct ccgacgcggc tctgagcacg caggaattca gcttcaacct
      481 ggaagagcag tgcttcttcg ctgagcgtct gggcagcggg cacggcatct gcagctgcgg
      541 gactggtgac taacctcgac tcacccgccc tctgggcggc gtaacacagt cgtgccaggc
      601 ggttcgtaag gatcgcctgg cacacttcta aattaatcgt tctgaaacaa ttccttgggg
      661 gaggatttgt gaagatttct gcgttcattt tgctctttgt ttttctcgcc tgttcgttcc
      721 tttctgcgca agttactcca gcttcgatta ctgctccttc cgtaatcgcc gacgtagatg
      781 gccatcggct cacggaatcc gatctcgacc ggaaaatgtc gggcagcctg ttgcaggccc
      841 gttatcagta ctacctgacc gaacgcaagg cgctggacaa gctcgtcgac gatgaactga
      901 tcgctctggc cgccgagcac cagcatatga ctacggacca gttcctggaa aaggaagtct
      961 acaaggacat caaagacccg acggaagacc agctgcaggt ctactacgag gggatggaga
     1021 gcaacgaatc gtacgaatcg ctgcacgacc ggctgatcga gcacatccgc gacctgcggc
     1081 gtgcgaaggc acgcgctgcc tatgtcaaga ctctgcgcac ccaggcaaac ctgcagatcc
     1141 ttctggcgcc ccccgtggca gacgtgaatg tggaaggcgc ggagctacat ggcgccaagg
     1201 acgctccagt tgtcctgttg gagtttgccg attatgagtg cccgtactgc cagaaggtga
     1261 atccactgct ggtgaagctc agggaagagt tcggcgacaa agtgaccatc gcctacaagg
     1321 acttccctct gcccatgcat cgcaacgccc agaaggcgtc agaagcggct cgttgcgccg
     1381 gagaacaagg caagttttgg gagtatcacg acctgcttta ttcaaccaag gcgctggaag
     1441 tcaatgacct gaagaagcac gcgcagactc tcaagctcga tgaggcacag ttcaacaagt
     1501 gcctcgacag cggcgcggaa gcagaaattg tcaagaaaaa cctggaagag ggcaagactc
     1561 tcggcctttc aggtactccc agtttcttcg ccaataatca ctttttcagt ggcggagtcg
     1621 actacaacac gttgcgcgag atggtggagc aacaaatcag ggcaagaaca accccgcagt
     1681 ctctgaccca aacggcgtcc accaagtagt ttgcagtcgg cgaggtacag agcgagcggt
     1741 gaaagatcgg ggcagctatg cgaacggacg gcttagtcgt aggcagacct tctggcactt
     1801 tgtctagaag gcagacgaag ggcagggaaa acactgccca cggcaagaat tccttcgggc
     1861 tgctcctggc acccgtggtt ttcgctctct tggcagtggc ggttctggca gcaccgccac
     1921 tcgccggcgc ccaggcggtc acggtgaccg ttagcccggg tgcggcgcag gtagtcaccg
     1981 ggagttcgct gcagcttacc agcaccgtga acggtgttgc gcccggtaca caaaaccagc
     2041 ttgtcacctg gtcgctctct ggtccgggct gcagcggatt gagttgcggc ctgatcaatg
     2101 ccgccggaca gtacaccgcg cccgccagcg ctccgaatcc gcccaccgtg tttgcgactg
     2161 cgacctcgat cttcaatcct ggcgcgagcg gaagtgcaac tctcacgatc gcggcgcctg
     2221 tcccagtcac cgtcaccgtg acgccgtcga cgttttccat caaacctgga gcccagcagc
     2281 aattcagcgc taccgtgacc ggtacgagca gcaaccaggt agtctggggt gcgagcggcg
     2341 ctgggtgctt tggtattcag tgcggaacta tcgactttaa cggtgtctac actgcgccga
     2401 ctcaggtgcc aaaccccgca gtggtgtctg taaccgcaac cttgctctct gatcagtcaa
     2461 agtcgggttc agcaaaagtc actatcggaa gcggcatcgc cgtatcggtg acgcctccca
     2521 ctcctcagat tctcttgggg caacagcagc agtttgccgc gaccgtaacc gggagcaaca
     2581 acacggccgt caactggacg gtttcgggtg cgggttgcgc gggggcaact tgtggaaaca
     2641 taacggcggg aggtctctac acggcacctg cggcgatgcc cggagcgccg accgtgacca
     2701 ttaccgcaac ttcagctgcc gacgtcaaca cgaatggcag cgctacactc acgctgttcg
     2761 ctaccccttc agtcagcgtt caaccgcact cggtccatgt gaaccctgga gcgaaacaag
     2821 tctttacag
//
LOCUS       DASPUL010000094         8829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1384688, whole genome shotgun sequence.
ACCESSION   DASPUL010000094 DASPUL010000000
VERSION     DASPUL010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8829)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384688"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VEJ00_02185"
     CDS             complement(<1..726)
                     /locus_tag="VEJ00_02185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXW99994.1"
                     /translation="MEKVEEIVVPQLATQTGKLPVSVIVAARNEEKNLPRCLASLREV
                     GEVYVIDSQSTDATPEIARSFGAKVAQFHYQGGWPKKRQWAMENLSFEYDWILLIDAD
                     EALTPELAEEIRSSIRIPDVNGYFISLQMYFLGRRLRHGDASFWKLALFRRGKGRYEC
                     RLRDQNPSMADMEVHEHIVVEGRTLRLKRPLIHHNVESLSHYIQKHDEYSNWEARVLL
                     QGEQGGTELPPAVFGTQAQRRRWL"
     gene            complement(739..2655)
                     /gene="asnB"
                     /locus_tag="VEJ00_02190"
     CDS             complement(739..2655)
                     /gene="asnB"
                     /locus_tag="VEJ00_02190"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXW99995.1"
                     /translation="MCGIFGIVAQNAQIPAGVLESGTESLAHRGPDDSGTILLRDSVP
                     DPTEVGLGNRRLAILDLSPLAHQPMHDAETGNWITYNGEIYNFREVRAELEQLGTTFI
                     SHSDTEVVLKAYARWGVECLTKFRGMFAFALWDAARRRLVLARDPMGIKPLYYAQSGS
                     YFLFASEVRTLLGTGLVPRSIDQAGLVNFLTFGSAYDPLTLIEGVRALPPGHTLIWER
                     GTLQQSSFWDLVGGREDSASLTEPILAANEESAEKELRPMLEEAVRSQLVSDVPVGVF
                     LSGGIDSSALVSIISRGGVTPATFSIVFREGDFSEAQHSRAVAAKFRTDHHEISVSQF
                     GVLDAIPGALRAMDLPTMDGINTYFVSQQARRAGVKVALSGLGGDEVFAGYSSFRTVP
                     RMEHLSQIWSRVPNAIRSPLAAAFFALRPENDQKRKLVTLARRNGRLVHPYFLSRMLF
                     TPGQRDLLFPAADAVAVDEASRSQYLSLDRARGLDPINRVSYLESRCYMLNTLLRDAD
                     FMSMSQGLEVRVPLIDHRLAKAILSLPGKWKLNGTPKKLLVAALAGSLPDEIVHRPKR
                     GFTLPFEHWMRQELREEIEPVLEAKRINNGLLGGLLDGVQVQNVWKDFLRGTVSWSRP
                     WSLYVLQRWCEQHL"
     gene            complement(2693..3853)
                     /gene="rffA"
                     /locus_tag="VEJ00_02195"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(2693..3853)
                     /gene="rffA"
                     /locus_tag="VEJ00_02195"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF008687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HXW99996.1"
                     /translation="MSYRIPFNRPCLAGNEYRYISEAIANGHASGDGPFTRRCHEFLE
                     KELGVARALLTTSCTHALEMAALLLDCGPEDEVIVPSFTFVSTANAFALRGARLVYAD
                     VRPDTLNLDESRLEDLITPRTKAIVVVHYAGVPCEMDAILAIAARHGVRVVEDNAHAL
                     FSRYKGGYTGTLGCLATQSFHETKNFTCGEGGALMINDPDLVERALVIREKGTNRSRF
                     FRGEVDKYTWVDLGSSYLPSDLLAAFLFAQFEAREQIQAKRQRIWHRYYQSLSDWAEG
                     NDVKLPVIPADCEQAYHMFYMILPSLEHRDSLITHLKSESILSVFHYTPLHLSEAGRK
                     FAARPSSCPVTEKISDRLVRLPFYNDLTGEEQDHVTNAVAKFGFYSVSRRAS"
     gene            complement(3850..4590)
                     /locus_tag="VEJ00_02200"
     CDS             complement(3850..4590)
                     /locus_tag="VEJ00_02200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXW99997.1"
                     /translation="MIGELETISISEIDSARFGINVARAVVGPENLTQILNCCAMENV
                     DLLIARCPAGDLATAQNMEMHGFRLMDTLVYYSFDLSRRAVPEDTGQFPVRDLRPGDQ
                     AQVRSVACAAFSGYMGHYHADPRLDRRQCDEAYSSWAERSCIPGTAADEVLVAEHDGQ
                     VLGFGTLRRNSPQEAEGLLYAVTPAWQGKGICRSLMIGSLRWCCAQGARRMIISTQVS
                     NVAMQKVWCRVGFEPSHSFHTFHKWFRP"
     gene            complement(4615..5661)
                     /locus_tag="VEJ00_02205"
     CDS             complement(4615..5661)
                     /locus_tag="VEJ00_02205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXW99998.1"
                     /translation="MKPEGSVLKGRVAGGAQPRQRLSVVIPVYRSEKILPELVSRLES
                     LLTAIADSFEVILVSDCSPDRSWDVIAGLAREHPWIRAINLMRNYGQHNALLCGIREA
                     RYDVIVTMDDDLQHPPEEIPKLLAMLAAGYDVVYGTPEHEQHGFGRDFASWISKIALQ
                     NVMGAEIARKVSAFRAFRAEVATAFTHYAGAFVSIDVLLTWGTNRFCLTRVRHEPRKE
                     GVSGYTFLKLVTHAMNMMTGFTTLPLQFASLVGFAFTLFGIGVLAYVVGRFLWLGGSV
                     PGFPFLASIIAIFSGAQLFALGIMGEYLARMHFRMMDRPSYVVRESIGLKDTAAATAD
                     LAVIPGGRHSQLGS"
     gene            complement(5755..6570)
                     /locus_tag="VEJ00_02210"
     CDS             complement(5755..6570)
                     /locus_tag="VEJ00_02210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXW99999.1"
                     /translation="MERLAEKTVGELPRAGGYDPAFFDQLTQVEDRHFWFRARNRLIF
                     ELARELGGSLNTCNRILEVGCGTGNVLRVLERACPQSLVVGLELWSDGLRHARHRSGA
                     ALVQADIRNLPFGGLFGLVGMFDVLEHIPDDRETLAAVWKSLAPGGRLLLTVPAHQSL
                     WSYFDEAAHHCRRYSQQDIREKLRAANFQIEFLSQFMACILPMVWIYRKMRDLRGHGG
                     AGGARSETTDEFRIVPVVNPILTMLLTLEAAWVGRGGRLPMGTSLVVVARKPA"
     gene            6767..8200
                     /locus_tag="VEJ00_02215"
     CDS             6767..8200
                     /locus_tag="VEJ00_02215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HXX00001.1"
                     /translation="MPTAFNPRASDSSDPAGSASRLAPGEAEHKANPEERKGQLSRHV
                     NVLLAVLLIAAASEFILRGPVRFLAQAPDWNDFILLYIPARAWLQGANLYSPEVYTQI
                     YTQMFAKPWSAAALRSHPAYPLNTFVLISPLAPFPWPIAQRAELALVLVLIGAMIFAI
                     SRLANLEGKAACLFGAAVLGLAPIHTGVATGNISITAAALACIAVWAVDAKKDVLAGV
                     LLAIVISLKPQIGILFLAYYLVRRKWLTFSLAVVGTGLIFLVGAVRMYALGLSWVTDF
                     VHNAHVWVRSNPTDDFASAGPMRYTLIDLQVPFYDLLGSKTAAVVLALAAAVTLGLIW
                     LILFLRARAPDDLLNLSILATLSLLPIYHRFYDASLLVLPIAWTLRANAPAYSRLRKA
                     CWLLFSLFLIPGSSILERMVSDGHVALRVLSSWWWQHVLMPHEAWALLLIVIALLAAM
                     SIRPGTAAERRPDASPTTKVVPVAKIG"
     gene            8380..>8829
                     /locus_tag="VEJ00_02220"
     CDS             8380..>8829
                     /locus_tag="VEJ00_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00002.1"
                     /translation="MALSVIVARIALLGVFPVPTPAVHDEFSYLLAADTFLHGRLANP
                     PHPMWVYLDTFHVLGHPTYASIYPPAQGGVLALGRLLGNPWIGVLFSMAVMCGAFTWM
                     LQGWFAPEWALLGGVLAIVRFGLFSYWVNSYWGGAVAATGAALVMGAL"
BASE COUNT         1828 a         2652 c         2565 g         1784 t
ORIGIN      
        1 gagccagcgg cggcgctggg cctgagtacc gaagactgcg ggaggtaact ctgttccgcc
       61 ctgctcgccc tggagcaaga cacgcgcttc ccagttcgag tattcatcgt gcttctggat
      121 atagtgcgac aacgattcca cattgtgatg aatcagcgga cgcttcagcc gcagtgtgcg
      181 gccttccact acgatgtgct cgtggacttc catatctgcc atcgacggat tctgatccct
      241 gagacgacat tcgtagcggc ccttgccacg gcggaataga gcaagtttcc agaagctggc
      301 gtcgccgtgc cttaacctcc gcccgaggaa gtacatctgc agcgagatga aataaccgtt
      361 tacgtcggga atccgaatgg agcttcgaat ctcttcggcc agttcaggcg ttaatgcttc
      421 gtcggcatcg attagcagaa tccagtcgta ctcgaacgaa agattctcca tggcccactg
      481 ccgcttcttg ggccagccgc cctgatagtg gaactgcgcc actttcgccc cgaatgagcg
      541 tgcgatttcg ggggtagcgt cggtgctctg ggagtcgatc acgtagacct cgcctacctc
      601 acgcagtgac gccaggcagc gaggcagatt cttctcctca ttgcgcgcag ctacgatcac
      661 gctcaccggc aacttgccgg tctgcgttgc caactgtggc actacgattt cttcgacctt
      721 ctccatacct aagacaacct acaaatgctg ttcgcaccag cgctgcaaca cgtagagaga
      781 ccacggtcgc gaccatgaaa cggttccgcg cagaaaatcc ttccagacgt tctgcacctg
      841 gacgccgtcg aggagtccgc cgagcaggcc attgttgatt cgtttcgctt ccagcaccgg
      901 ctcgatttct tcccgcagtt cctgccgcat ccagtgctca aagggcagag tgaagccgcg
      961 cttgggacga tgaacgatct catccggcaa cgacccggct agcgcggcca ccagcagctt
     1021 tttcggcgtg ccgttcagct tccactttcc gggaagagaa agtatcgctt tcgccagacg
     1081 atgatcgatg agaggaacgc ggacttccag gccctgggac atgctcatga aatcggcgtc
     1141 gcgcaacagc gtattgagca tataacagcg cgactcgaga taggaaacgc ggttgatggg
     1201 atccaggccg cgggcgcggt ccaggcttaa atactgggac ctggatgcct cgtctaccgc
     1261 aaccgcgtcg gcagccggga acaggagatc gcgctggccg ggcgtgaaca gcatgcgcga
     1321 gaggaagtag gggtgcacga gccgaccgtt gcggcgggcc agcgtgacca gtttgcgttt
     1381 ctggtcgttc tcgggacgca gtgcaaaaaa ggccgctgcc agcggacttc gaatcgcgtt
     1441 gggaacacga ctccagatct gagaaaggtg ctccatgcgc ggaacggtcc ggaagctcga
     1501 gtagcctgcg aaaacctcgt cgccacccaa acctgacagc gcaactttca caccggcgcg
     1561 gcgagcctgt tgcgacacga aatacgtgtt gattccgtcc atggtaggca aatccatggc
     1621 ccgcaaggcg ccggggatgg cgtccaggac accgaactgc gagacgctga tctcgtggtg
     1681 atcggtgcga aacttcgcag ctacagcacg ggaatgttgt gcctcggaga aatcgccctc
     1741 gcggaagaca atcgagaacg tagccggcgt aacgccccct cggctgatga tgctgaccaa
     1801 tgcgctggaa tcgatacctc cggagagaaa aacgccaacg ggaacgtcgc tgaccaactg
     1861 cgagcgcacc gcctcctcga gcatcggacg gagttccttt tccgcgctct cctcgttcgc
     1921 ggccaaaatc ggctctgtaa gcgacgcgct atcttcgcgg ccgccaacca gatcccaaaa
     1981 cgaggattgt tggagcgttc cgcgttccca aatcagcgtg tgaccaggag ggagcgcgcg
     2041 aacgccttca atcaatgtga gcggatcgta ggcggagccg aaagtcagga agttaaccaa
     2101 tccggcctgg tcaatgctgc gaggaacaag tccggttccg agcagcgttc ggacttctga
     2161 agcgaagagg aaatacgaac cggactgagc atagtagagc ggcttaatgc ccatgggatc
     2221 gcgcgccagt actagccggc gtcgggccgc atcccagagg gcaaaggcaa acatcccgcg
     2281 gaatttcgtg agacactcga cgccccaacg cgcgtacgcc tttaagacaa cctcagtgtc
     2341 ggaatggctg atgaaagtgg tcccgagctg ctcgagttcc gcgcgcactt cacgaaagtt
     2401 gtagatctcg ccgttgtagg tgatccagtt gccggtttcc gcgtcgtgca tgggctgatg
     2461 ggcgagaggg gagagatcga gaattgcaag acgccggtta cccaggccga cttccgtcgg
     2521 gtcaggcacg gagtcgcgca gaagaatcgt gcccgaatca tctggcccac gatgcgcgag
     2581 cgattccgtc ccgctctcga gcacacccgc cgggatctgc gcgttctgtg cgacaatgcc
     2641 gaaaattccg cacatggaaa aaggctaact catgagccgg ctcaggtgag gcctaactgg
     2701 ctcgccggct gaccgaatag aacccaaact tcgccaccgc attcgtgacg tggtcttgct
     2761 cctccccggt cagatcattg tagaagggca agcgaaccag ccggtcactg atcttttccg
     2821 tcaccgggca actcgatgga cgagcggcga acttgcgccc cgcctccgaa agatggagcg
     2881 gcgtgtagtg gaacacgctc aggatgcttt cggacttgag atgcgtgatc aacgagtcgc
     2941 ggtgctcgag tgaaggcagg atcatgtaga acatgtggta ggcctgctcg caatcggcag
     3001 gaatgacggg cagtttcacg tcgttgccct cggcccaatc ggacagtgac tggtaatagc
     3061 gatgccagat gcgttggcgc tttgcctgaa tctgttccct ggcttcgaac tgcgcaaaca
     3121 gaaaggccgc cagcaaatcc gaaggcagat agctggagcc gagatcgacc caggtgtatt
     3181 tgtccacttc gccgcggaag aagcgactgc ggttggttcc cttttcacga atcacgagcg
     3241 cacgctcaac caagtcagga tcgttgatca tcaacgcccc accctcgccg caggtgaaat
     3301 tcttggtctc gtgaaagctc tgtgtggcaa ggcacccgag cgttccggta tagccaccct
     3361 tgtagcggga gaacaacgcg tgggcattat cttccacgac tcgaactccg tggcgcgccg
     3421 caatcgcgag aatcgcgtcc atttcgcatg gcaccccggc gtaatgaacc acgacgatcg
     3481 ccttggttcg cggcgtaatc aggtcctcga ggcggctttc atccagattt aacgtgtcag
     3541 gccggacgtc agcgtagacg aggcgcgcgc cccgtaaagc gaaagcattg gcagtagata
     3601 caaacgtaaa cgatggaacg atgacctcgt cttcggggcc gcagtcgagc agaagcgcgg
     3661 ccatctccag ggcatgcgta catgaggtcg tcagcaaggc tcgggcaacc cccaactctt
     3721 tttccagaaa ctcgtggcac cgcctggtga acgggccatc gcccgaggca tgtccgttcg
     3781 cgatcgcctc cgagatatac ctgtactcgt tccctgcaag gcagggccgg ttgaatggaa
     3841 tgcgatagct catgggcgaa accacttgtg aaacgtatgg aaggaatggc tcggctcgaa
     3901 gcccactcga caccacactt tctgcatggc gacattactc acctgagttg aaatgatcat
     3961 gcgtcgcgcg ccctgagcgc agcaccatcg caggctcccg atcatgaggg aacggcaaat
     4021 tcccttcccc tgccacgctg gcgtaaccgc gtagagcaga ccttcagcct cctggggcga
     4081 attccggcgc aacgtgccaa aaccgagcac ctgaccgtca tgttcagcta ccagaacctc
     4141 gtcggcggct gtaccgggaa tgcacgaacg ctcggcccaa gagctgtacg cctcatcgca
     4201 ctggcgccgg tccaggcgag gatcggcgtg ataatgtccc atataacctg agaatgcagc
     4261 gcaggcaacg cttcgaactt gggcctggtc ccccggccgg agatcgcgca cgggaaactg
     4321 gccggtgtct tcaggaaccg cccggcggga caagtcgaaa ctgtaataga ccagcgtgtc
     4381 catcagtcgg aagccgtgca tttccatgtt ctgcgccgtc gcgaggtcgc cagcagggca
     4441 gcgggcaatc agcaggtcca cgttctccat tgcgcagcag ttcaggatct gggtgagatt
     4501 ctcaggtcca acgacagccc gcgcgacatt gataccaaag cgcgcgctgt ctatctccga
     4561 aatcgagatg gtttccaact caccgatcat ctggcagtgc cctgtctccg ttcatcaaga
     4621 acccagttgc gaatgccggc ctccgggaat cacggctaaa tcggctgtgg cggctgccgt
     4681 gtccttcaac ccgatgcttt cccggacgac gtaactgggg cgatccatca tgcgaaagtg
     4741 catgcgtgcc aggtattctc ccatgattcc caatgcgaac aactgagcac ccgagaaaat
     4801 ggcaatgatc gacgcaagaa acgggaatcc tggtacgctg cctccgagcc acagaaatcg
     4861 gcccaccacg taagccagca cacctatgcc aaagagcgta aaagcaaacc ccaccaagct
     4921 ggcaaactgc aggggcagcg tggtgaatcc cgtcatcatg ttcatggcat gcgtaacgag
     4981 cttgaggaag gtataaccgg acacaccctc tttccggggc tcgtgccgca cccgcgtcag
     5041 gcaaaagcga ttcgtccccc aggtgagcaa aacgtcaatg gaaacgaagg ctccggcgta
     5101 atgggtgaag gccgttgcca cctcggcccg aaaagcgcga aacgcgctca ccttgcgggc
     5161 gatttcagct cccattacat tctgcaaggc gatcttggag atccaggaag caaagtcgcg
     5221 gccgaagccg tgctgctcat gttccggcgt gccgtaaacg acgtcgtaac cggccgccag
     5281 catcgccaga agttttggga tctcctcggg aggatgttgc agatcgtcgt ccatggtgac
     5341 gatcacgtcg taacgggcct cccggattcc gcacaggagc gcattgtgct ggccgtaatt
     5401 gcgcatgaga ttgatggcac gaatccaggg atgttcccgc gccagcccgg cgatgacgtc
     5461 ccagctgcgg tcagggctgc aatcgctgac gaggatgacc tcaaaactgt cggcgattgc
     5521 ggtgagcagc gactccagcc ggcttaccaa ttccggcagg attttctcgc ttcggtagac
     5581 cggaatgacc acggacaatc gctgccgcgg ttgcgcaccg ccggcgactc ttcccttcag
     5641 cactgaccct tccggcttca atcggaatat tctcctcaat cgaggaactg cccaccaggc
     5701 ttagtgtgca tgatacagac tattcttgat ccagtcatgg ctcctgtggc cggttcaggc
     5761 cggtttgcga gcgacgacaa ccagcgacgt ccccataggc aaacggcctc cacggcccac
     5821 ccaggcagct tcgagggtca gcagcatagt caggatcgga ttcaccaccg ggacaatccg
     5881 gaattcgtct gttgtctcgg accgcgcccc tcctgccccc ccgtgcccac ggaggtcacg
     5941 cattttgcgg tagatccaca ccatcggtaa gatgcaggcc atgaattggc tgagaaattc
     6001 gatctggaag ttggccgccc taagtttctc gcgaatatcc tgctgggaat agcgccggca
     6061 gtgatgagcg gcttcatcaa agtagctcca caaggactgg tgagctggaa cggtaagcaa
     6121 gagccgtccg ccgggcgcca acgatttcca caccgccgct agtgtctcgc ggtcatcggg
     6181 gatgtgttcg agcacgtcga acatgcccac aagaccgaac agtccgccaa agggcaggtt
     6241 acggatatcg gcctgaacca gcgccgcacc ggatcggtgg cgagcatgtc gcaacccgtc
     6301 agaccagagt tccaggccga ccacgaggga ctgcgggcac gctctctcca gtactcgcag
     6361 cacgtttccc gtgccacatc cgacctccaa tatccgattg caggtgttga gcgacccacc
     6421 cagttcgcgc gccagctcaa aaatcaggcg gtttcgagca cgaaaccaga agtggcgatc
     6481 ttcgacctgc gtcaattgat cgaagaaggc cgggtcataa cccccggctc ggggcagctc
     6541 cccgactgtc ttttctgcga gtctctccaa cccttcggcg acttttggct ctaactctga
     6601 agtcaggcgc atcacagcac gggaagatgc aattctgttt caacaactga tccgtgacga
     6661 aaaacgtacc gctcaaacga tgtgctattg tactggcagt tcgcggctaa ccaaagtaaa
     6721 tgctctcatt gccgcgcttc acacggtcct gcggggcagt ccagaaatgc caactgcatt
     6781 caatcccaga gcgtcggatt ccagcgatcc ggcgggctcg gcgagtcgcc tcgcaccggg
     6841 cgaggcagag cacaaggcaa atccagaaga gcgaaaaggg cagcttagtc ggcacgtcaa
     6901 tgttctgctt gcagtgttgc ttatcgccgc cgcgagcgaa ttcattctcc gcggcccagt
     6961 ccggtttctt gcccaggcgc ctgactggaa cgacttcatt cttctctaca ttccggctcg
     7021 cgcctggcta cagggagcaa acctctatag ccccgaagtg tacacgcaga tctacacgca
     7081 gatgttcgca aaaccgtgga gcgcggctgc gctcaggagc catccggcat atccactgaa
     7141 taccttcgta ttgatctcgc cgctggcgcc gtttccctgg cccattgccc aaagagcaga
     7201 gttggcgctg gtgttggttc tgatcggagc gatgatcttc gccataagtc gattggcaaa
     7261 cctggaaggc aaggcagctt gtcttttcgg ggccgccgtg ctggggctgg cgccgatcca
     7321 caccggagtg gccacgggca acatcagcat aacggcggca gcccttgcct gcatcgcggt
     7381 ctgggcagtt gacgccaaga aagatgtctt agccggagtg ctgctggcga tcgtgatcag
     7441 tctgaagccc cagatcggaa ttctgttcct ggcttactac ctcgtgcgcc gcaagtggct
     7501 cactttcagt ttggccgtgg tcgggacagg actgatcttt ctggtcggcg ctgtccggat
     7561 gtacgcactg ggcctgagtt gggtgaccga cttcgtccac aatgcacacg tctgggtccg
     7621 ctcgaacccg accgacgact tcgcatcggc tggcccgatg cgctacacgc tcattgatct
     7681 gcaggtgcct ttctatgatc tgttaggctc gaagactgca gccgtcgtcc tcgcacttgc
     7741 cgctgccgtg acactgggtc tgatctggct cattctcttc ctgagagctc gcgcgccgga
     7801 tgacttgctg aacctcagca tactggcgac tctgagcctc ctgcccattt accaccggtt
     7861 ctatgacgct tctctgctgg tgctgcccat tgcctggacg ctgcgtgcaa acgctcctgc
     7921 atattcacgg ctgaggaaag cgtgctggct gctgttctcg ctcttcctca ttcccggttc
     7981 cagcattctg gagcgcatgg tctcagacgg tcacgttgcc cttcgcgtgc tgagttcctg
     8041 gtggtggcag catgtgctga tgccgcacga ggcctgggcg ttgctgctga tcgttattgc
     8101 gctcctcgca gcgatgtcga ttcggccggg gactgcggcg gaacgccgtc cggatgcatc
     8161 ccctacgaca aaagtcgtcc cagttgctaa gatcggctga atctttgcaa tgaaatccct
     8221 tgtcctcgtg gggtctattt gtctcatttc caggatctga ttctgattgg gatattcgtg
     8281 ctggcggtgg cggcgcctcg attggcggat cgcccgttca gttcggcgga aaaattcgtc
     8341 tctgcctttg cgcggtgtag acacagagcg gtcctgatag tggcgctatc ggtaatcgtc
     8401 gcccgtatcg cgcttcttgg cgtcttcccg gtgccaactc ctgcggtcca cgatgagttc
     8461 agctatctgt tggctgcgga cacttttctg catgggaggc tggcgaaccc gccgcacccc
     8521 atgtgggtct acctcgacac gtttcacgtg ctcggacacc caacgtacgc gtcgatctat
     8581 ccgcccgctc agggcggagt gctagcgctg gggcgtttgc tgggtaatcc gtggattggc
     8641 gttctgttca gcatggccgt catgtgcggc gcgttcacct ggatgctgca aggctggttc
     8701 gcgcccgaat gggcgctgct gggaggagtt cttgcgatcg tgcgcttcgg gctcttcagc
     8761 tattgggtga acagctactg gggtggcgca gttgcggcga caggagcggc gttggtgatg
     8821 ggcgccttg
//
LOCUS       DASPUL010000095         3800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_16016, whole genome shotgun sequence.
ACCESSION   DASPUL010000095 DASPUL010000000
VERSION     DASPUL010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16016"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..2386
                     /locus_tag="VEJ00_02225"
     CDS             179..2386
                     /locus_tag="VEJ00_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00003.1"
                     /translation="MNKFLNKTAEATAWIYNPWLDLIVGCGAWSTPLLLISYFAVASS
                     ARTWSVVFYILAFFFNYPHYMATIYRAYHRAEDFHKYRVFTVHITALMLLTLVLSHSW
                     LRILPWIFTIYLTWSPWHYSGQNYGLFMMFARRAGADPDKATRRALYGAFIVSYLILF
                     LGFHTGPSADPLFLSLGIPALVSRWEQMILGIAFVALSGFGLSRLARATGWRKLTPSL
                     TLFSSQCLWFLVPTAIAFIKGLEIPQNRYSTGVLAVMHSAQYLWITSYYARREARSGA
                     AEAAPRSWRPLAYFGVLIVGGIALFVPGPWLASRAFHHDFTASFLIFTALLNIHHFIL
                     DGAIWKLRDGRIASLLLNSREGIADAASQAGGRIAAACRWLAGSTSAARALRVSAAAL
                     LLIWGTIDQMRYYLGLHSDDLRSLQRAAALDSTDSALETRLGRKALDVGDAQQAEAAW
                     RRAIAVNPADPAPRQELLRLLIDEKRLDEALKLTDASLQYASRDANLLFDRGLLAQQL
                     GQNDAARFAWQQALTVDPNHSLARLYLANELDREGKAREAATDYEMFLQGLAHQSRQA
                     RPEPEKVIAIALRMADCQARSSQTDRAVKSYHLAEKLAVQTKLPKLESVADVNEAALQ
                     AQTGKLDDALQLYQHALQLDKADGDPSAAAQDWFAYGSFLNDAGFSARLAYACLLKSE
                     SVAQSLPDPAVQDSHSAARGQLQKRLEKQLGTESAKVRRDLNSAIEQALALRR"
     gene            2511..3326
                     /locus_tag="VEJ00_02230"
     CDS             2511..3326
                     /locus_tag="VEJ00_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00004.1"
                     /translation="MADEGEHLHMEHQSNAGKWILTILAVLVVAGFAYAHYTTYSSVQ
                     KLTKDLDASQAQVKELQNRMQTAEAQEETLARQAGMTKKELAQRAAALQAEERATASR
                     LEEEQKQQIGQVTGDIAGVKTDVGGVKTDVAATKADLEATKARLQTAVGDLTGQSELI
                     ATTRADLEVLKHKGDRNYYEFTLLRGAKPQPVSTVSLQLKKADAKRGKFTMNVTSDDK
                     TIEKKDRNVAEPIQFYSGREHMLFELVVWTVDKNKATGYLSTPKNAPVPVTAN"
BASE COUNT          749 a         1253 c         1103 g          695 t
ORIGIN      
        1 acccctaggg gcgtacgaga tgcattctgc gcgcccatta ctttcgttac ccacgccgca
       61 cccatccggt tacgcggcca cgcattttaa gcccctataa tcgaaccaag ccacacagat
      121 cagcgatacg aacgagcctc gccggctcac cggtcgcaga ctgtagccaa agcccggaat
      181 gaacaaattc cttaacaaaa cggccgaagc aaccgcctgg atctacaatc cgtggctcga
      241 tctgatcgtc ggctgtgggg cctggtcgac gcccctgctg ctgatttcgt attttgcagt
      301 ggcctcgagc gcccgcacgt ggtcggtcgt cttctacatc ctcgcgttct tcttcaacta
      361 cccgcactac atggcgacga tctaccgcgc ctaccatcgc gccgaagact tccacaaata
      421 ccgcgtcttc accgttcaca ttacggcgct gatgctgctg acgctggtgc tctcgcattc
      481 ctggctgcgc attcttccgt ggattttcac catctatctg acctggagcc cgtggcacta
      541 cagcggccag aactatggct tgttcatgat gtttgcccgc cgcgccggcg ccgatcccga
      601 taaagcaacg cgccgggctt tatatggcgc cttcatcgtc tcgtacctga ttctgtttct
      661 cggcttccat accgggccct cggccgatcc actctttctg tcgctcggaa ttccagcgct
      721 ggtgagccgc tgggaacaga tgatcctggg aatcgccttc gtcgcgctgt cgggcttcgg
      781 tctgtcacgg ctcgcgcgtg ctacgggatg gcgcaagctg acgccctcgc tcaccctgtt
      841 ctcctcgcaa tgcctctggt ttctggtgcc cacggcgatt gcgttcatca agggcttgga
      901 gattccgcag aatcgctaca gcacgggcgt gctcgccgtg atgcattccg cccaatatct
      961 ctggatcacc agctactacg cacgccgcga ggcacgctcg ggcgccgcag aagcggctcc
     1021 gcgcagctgg cggccgctgg cttacttcgg cgtgctgatc gtaggcggca tcgccttgtt
     1081 cgttcccgga ccatggctgg ccagccgcgc cttccatcat gacttcaccg ccagcttcct
     1141 gatcttcacc gctctgctga acattcatca cttcatcctc gacggcgcga tctggaagct
     1201 gcgtgacggc cgcatcgcat cgttgctcct caattcacgg gagggcatcg cggacgcagc
     1261 atcccaagcc gggggccgaa tcgctgccgc ctgtcgctgg ctcgcgggga gcacgtccgc
     1321 cgcgcgtgcg ctgcgtgtga gcgcggccgc cctgcttctg atctggggaa ccatcgacca
     1381 gatgcgctac tacctcgggc tgcactccga cgatctgcgt tcgctacagc gcgccgccgc
     1441 actcgattcg accgacagtg ctcttgagac caggctgggc cgcaaggcgc tcgacgtcgg
     1501 cgacgcgcag caggccgaag ccgcctggcg gcgggcgatc gcagtcaacc ccgccgatcc
     1561 cgcgccgcgc caggaattgc tgcgcctgct catcgatgag aaacggctcg acgaggcgct
     1621 taagctgacc gacgcatcgc tccaatatgc gtcccgcgat gccaaccttc tgttcgatcg
     1681 cggcctgctc gcgcagcaac tcggccagaa cgatgccgcc cgcttcgcct ggcagcaggc
     1741 cttgaccgtc gatcccaacc actcgctcgc ccgcctgtac ctggcaaacg aactcgatcg
     1801 cgaaggcaag gctcgcgaag ccgccaccga ctacgaaatg ttccttcaag gtctggccca
     1861 ccagtccagg caagcccggc cggaaccgga aaaggtgatc gccatcgcgc tgcgcatggc
     1921 cgactgccag gcgcgctcgt cgcaaaccga tcgcgccgtg aagtcctatc acctggccga
     1981 gaagctggcc gtgcagacga agctgcccaa actcgaaagc gttgccgacg tgaacgaggc
     2041 agcgctccag gcgcaaaccg gcaagctgga cgacgcgttg cagctctacc agcacgctct
     2101 gcagctcgac aaggctgacg gcgacccgag cgccgctgcg caggattggt tcgcctacgg
     2161 cagttttctg aatgatgccg gattttctgc gcggctcgcc tacgcctgcc tgctcaagtc
     2221 ggagagtgtc gcgcaatcgc tgcccgatcc ggcggttcag gattcgcatt ccgccgcgcg
     2281 cgggcagttg cagaaacggc tggagaagca gctcggcacc gagagcgcga aggtacgccg
     2341 cgatctcaac tcggccatcg agcaggccct ggccttgcgg cgctagagca ttggatggga
     2401 catctgctgc ggaaaacgcg gcatcccatg agctaattga caccgttgga accgtactga
     2461 acaattgggt attcccacag ttttgttagc ttcccgagga ggaaggcact atggccgatg
     2521 aaggcgaaca tctacacatg gaacatcaat cgaacgccgg caagtggatc ctgacgatcc
     2581 tggccgtcct ggtggtcgcg ggctttgcct acgcgcacta caccacctat tcgtcggtgc
     2641 agaaactcac caaggatctt gacgccagcc aggcgcaggt caaggaattg cagaatcgca
     2701 tgcagaccgc cgaggcgcag gaagagacgc tggcccgcca ggccggcatg accaagaagg
     2761 aactggccca gcgcgccgcc gcactacagg cggaagagcg cgccaccgcc agccgcctgg
     2821 aggaggagca gaagcagcag atcggccagg tgacgggaga catcgccggc gtgaagaccg
     2881 atgtgggcgg agtcaagacc gacgtggccg ccacgaaagc ggatctcgag gcgacgaaag
     2941 ccagactgca gactgccgtc ggcgatctga ccggccagtc cgagctcatc gccacgacgc
     3001 gtgccgatct cgaagtcctc aagcacaaag gcgaccgcaa ctactacgag ttcacgctgc
     3061 ttaggggcgc caagccgcaa ccggtttcga ccgtcagcct ccagctcaag aaggccgacg
     3121 ccaagcgcgg caagttcacc atgaacgtga cctccgacga caagacgatc gagaagaaag
     3181 accgcaacgt cgccgaaccc atccagttct actccggacg cgagcacatg ctctttgaac
     3241 tggtggtatg gaccgttgac aagaacaagg ccacgggata cctgagcaca cccaagaacg
     3301 cgcccgtgcc ggtgacagcg aactgattgt cgattggcga ttgacgattg ccgatttgag
     3361 ggcggctgat tagcaagtga aaaacggaat cctgcatcag gcctcgcttg cgaggaaaac
     3421 gggaagggca cgagttcact cgtgccgcca ggtcggcgga aacgcgtcgc gcttcagcgc
     3481 ctgagggtcg ctttgacaag cgtacgggtg cgggctgcgg aagaagcgtt ccccagcggc
     3541 taaagccgaa tcgttttgtg caacgtaccg acgactgaaa gtcgtgccct tcccaaatca
     3601 gcagaactcg gactctgttt tttgggaacg cgcatgatca acccagctct tttccccggc
     3661 agccatattc tgttgcagat cgtgctgcgc accggcgtga tctacctgct ggtgctgatc
     3721 ggcgtgcgcc tcagtggcaa gcgcgaagtc gggcagatga ccccgttcga cctcacgctg
     3781 cttctgctcc tatccaactc
//
LOCUS       DASPUL010000096         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1384989, whole genome shotgun sequence.
ACCESSION   DASPUL010000096 DASPUL010000000
VERSION     DASPUL010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384989"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VEJ00_02235"
     CDS             <1..315
                     /locus_tag="VEJ00_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXX00005.1"
                     /translation="VFANLIDNAMKYGRSGGRIVLGARRAPRGIEFYVKDFGAGISSE
                     HLPRLFERFYRVDKARSRESGGTGLGLAIAKHIMLAHGGSIRAESELNHGSTFLFTLP
                     TA"
     gene            493..3021
                     /locus_tag="VEJ00_02240"
     CDS             493..3021
                     /locus_tag="VEJ00_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXX00006.1"
                     /translation="MSPNRVVGIVVFALCLAGIAWAQGGATGAISGTVQDASGAAVAG
                     AKVDVISDATNLSVRQVTSDSAGLFTVPLLPVGSYTIQVTATGFATTRFPGILVSITE
                     TTRMTASLKPATIKEVVEVQYEVPPVETTAATTGESLNNTTITELPLATRNFQQLLTL
                     SAGASSDLNGASQLGRGGVVMHVNGGREDNNNYLIEGISAADYAFGELTYTPLPNPDS
                     IQEFKVSTSLYDASQGRNGGGNINATMKSGTSGFHGDLWEYFRNTSLDAHDYFIGKFD
                     LKQNIFGGDFGGPVGKEAKFGFFYVNYQGTRQRSGDSAGTFINTQIPVLPTARDQQTL
                     INTFFCGAACGGVTPPICTGGNPCLDTVAVNLLQFPGTQFGGGGTGCGASGTQSCLIP
                     TVPGTPGFTLVNGVPTLNFGPLILTDPGKFRDDQFTANWDRDFRGGKDHIAERFFWSD
                     SDTFEPYGADSFGIQTGGQPGTNNLNFPLDIPLHSRFGSITETHTFTNSLINEFRFGV
                     NIISDRLSNQAPVTNAQVGIDLPTATGVNGQAGDPNIYRLQFATWGFGAYPTQLQSAL
                     SDNLTWIDTLSWTRGAHQLRLGGEFDHVSLRRSLPIADNGLVFFTPPNVNTDFQSFLA
                     GSPFFGEGGGGLGNHDYRIPSYSWFAQDDYRVRKDLTVNLGFRNEFVGAPYDLLCHTG
                     NTNPELAITTGQPYVWPKCVNKFGLTGVTGTLNSAGLSNEYATVWEPRIGFAYDLGGR
                     HTTSIRGGYGIYSVREDLGAADNLAITPPAYPFGVNFGPAIFGPCQQTASCLATLFAA
                     SNNNPAFALPGVCASRRSSAGRDTNATVCAPCLVPSEFRLPLQH"
     gene            3098..>3702
                     /locus_tag="VEJ00_02245"
     CDS             3098..>3702
                     /locus_tag="VEJ00_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00007.1"
                     /translation="MPLHWVVGTTQQWNFTVQRALGHEWFAELGYVGTKGSRLRSTYD
                     PDQATLATAANPITLTGQACANLTTAGQSCTIVDSTGENVSARAPFIGIAPSDFEDFA
                     PNSDSHYNALQATLAHHFSKGLYFQSAYTYAKSIDDVSTASVAFVTRVNDQTNGSASR
                     GLSDFDRRQRFVASAVYQIPFFDSATGVEKAALSGWEASGV"
BASE COUNT          711 a         1256 c         1035 g          700 t
ORIGIN      
        1 gtctttgcca acctcatcga caacgccatg aagtacggcc gctccggcgg acgcatcgtg
       61 ctgggagcac gccgcgcccc ccgcgggatc gaattctatg tgaaggattt cggcgccggc
      121 atctcttctg agcatctgcc ccggctattc gagcgcttct atcgcgtcga caaagcgcgc
      181 tcccgcgagt ctggcggaac cggtctcggc ctcgccatcg ccaagcacat catgctggcg
      241 cacggcggct ccatccgcgc cgaaagcgaa ctcaaccatg gcagcacgtt tttgttcacc
      301 ctccccacgg cctgagctcg cggtccaatc gcctcgcggc ctgtggaaaa agaggttgac
      361 agtcgcttac aagaggcggc aaactcgcgg aatccgtcgg ttctaaaagc tcgcatgcgg
      421 cgtgggccgc tggcgagttc ccgttgtcac cgacacggat tctttcttaa agccaaactg
      481 gggagcaaga ccatgtcgcc gaatcgggtg gtaggaattg ttgtctttgc gttgtgcctg
      541 gctggcatcg catgggcgca gggcggagcc acgggggcca tcagcggtac cgtccaggac
      601 gcctccggcg ccgccgtcgc cggcgccaaa gtcgacgtca ttagtgacgc cacaaacctg
      661 tctgttcgcc aggtgacgtc tgattccgcc ggtttgttca ccgtgccgct gttgcccgta
      721 ggcagctata cgatccaggt gaccgcgacc ggattcgcca ccacccggtt cccgggaatt
      781 ctcgtcagca tcaccgaaac cacccgcatg acggcctcgc tgaagcccgc cacgatcaag
      841 gaagtcgtcg aggtgcaata cgaggttcct ccggtcgaga ccactgcggc gaccactggc
      901 gaatcgctga acaacacaac gatcaccgag ctcccgctgg ccacacgcaa cttccagcag
      961 ttgctcacac tttcggccgg cgcatcgtcc gacctcaatg gcgcttcgca gcttggccgc
     1021 ggcggggtcg tcatgcatgt taacggcggc cgcgaagaca acaacaacta cctgatcgaa
     1081 ggcatctcgg ccgctgacta cgctttcggc gaactgacct acactccgct gcccaaccct
     1141 gactcaattc aggagttcaa ggtcagcacc agcctctacg acgccagcca gggacgcaac
     1201 ggcgggggca acatcaacgc caccatgaag agcggcacct cgggctttca cggcgacctg
     1261 tgggaatact tccgcaacac ctcgctcgac gcccacgact atttcatcgg caaatttgat
     1321 ctcaagcaga acatctttgg cggcgacttc ggcgggcccg tgggcaagga ggcgaagttc
     1381 ggtttcttct acgtgaacta ccagggcacg cgccagcgca gcggcgactc ggcgggaacg
     1441 ttcatcaaca cgcagatccc ggtgctgccg acagcgcgcg accagcagac gttgatcaac
     1501 accttcttct gcggcgcggc ctgcggcgga gtaacaccgc cgatctgcac cggcggcaac
     1561 ccgtgtcttg atacggtggc cgtgaacctg ctgcagttcc cgggcacgca gttcggcggg
     1621 ggcggcaccg gctgcggggc ttcggggaca caatcgtgtc tcattccgac agttccaggc
     1681 acaccgggtt tcaccctagt aaatggagtg cccacactca atttcgggcc gctcatcctg
     1741 accgatcccg gcaagttccg ggacgaccaa ttcaccgcca actgggatcg cgacttccgc
     1801 ggtggaaaag accacatcgc cgagcgcttc ttctggtccg attcggacac gttcgagccc
     1861 tacggcgcgg attcgttcgg catccagacc ggaggccaac cgggcaccaa caacctgaac
     1921 ttcccgctcg acatcccgct gcacagccgc ttcggcagca ttaccgagac ccacaccttc
     1981 actaattcgc tgatcaacga attccgcttc ggcgtcaaca tcatcagcga ccgcctgagc
     2041 aaccaggcgc cggtcacaaa tgcgcaggtg ggaatcgatc tgcctaccgc gacaggcgtt
     2101 aacggccagg ccggggatcc caacatttac cgccttcagt tcgcaacctg gggattcgga
     2161 gcctatccca cacagctgca gtcggctctc tccgacaacc tgacctggat cgacacactc
     2221 agctggacca ggggtgcaca ccagcttcgc ttgggcggcg agttcgatca tgtctcgctc
     2281 cgccgcagcc tgcccattgc cgacaatggc ctggtgttct tcaccccgcc gaacgtgaat
     2341 acagatttcc agagcttcct ggctggttcg cccttctttg gcgagggcgg cggcggactc
     2401 ggcaatcacg attaccgtat ccccagttac tcctggttcg cccaggacga ctatcgcgtg
     2461 cgcaaggact tgaccgtcaa tctgggattc cgcaacgaat tcgtaggcgc gccctatgat
     2521 ctgctctgcc acaccggcaa cacgaatcca gagctcgcga tcacgaccgg ccagccctac
     2581 gtctggccga agtgcgtgaa caagttcgga ttgactggcg ttaccggcac tctgaattcc
     2641 gccggcctca gcaacgagta tgcgacggtt tgggaacccc gcatcggctt cgcctatgat
     2701 ctgggcggcc gccacacgac gtcgatccgt ggcggatacg gcatttactc cgtccgcgaa
     2761 gatttgggcg ccgccgacaa tctcgcgatc actccgcccg catatccgtt tggagtgaat
     2821 ttcgggcctg ccatcttcgg tccatgccag caaaccgcaa gctgcctggc taccttgttt
     2881 gccgccagca acaacaaccc ggcgtttgcg cttcccggcg tttgcgcttc ccggcgttcc
     2941 agcgctgggc gcgacaccaa cgcaaccgta tgcgcccctt gcctcgttcc ttcagagttt
     3001 cgccttcccc tgcaacatta acggggctcc ggctgtcaac gccgacgagt gcggggcgaa
     3061 cttcaatggc acagtgaatt ctcttatcga gctgaccgtg cccctacact gggttgtcgg
     3121 aactacccag cagtggaact tcaccgttca gcgcgcgctc ggacacgagt ggttcgcgga
     3181 actcggttac gtcggcacga aggggtcgcg cctgcgctcc acgtacgacc ctgaccaagc
     3241 cacattggcc accgcggcca acccgatcac cctgaccggc caggcctgtg cgaatctcac
     3301 gaccgccggg cagtcgtgca cgatcgtgga ttcgaccggc gagaatgtga gcgcgcgagc
     3361 tccgttcatc ggcatcgcgc cctcggactt cgaagatttc gctcccaact ccgactctca
     3421 ctacaacgcc ctgcaggcaa cgttggcgca tcacttctct aagggcctgt atttccagag
     3481 tgcctacacc tacgcgaaat cgattgacga cgtttccact gcgtcagtcg ctttcgtgac
     3541 ccgcgtcaac gatcagacca acggcagcgc ctctcggggc ctttctgact tcgaccgccg
     3601 ccagcgtttc gtcgccagcg ccgtgtatca gatcccgttc ttcgacagcg ccaccggcgt
     3661 cgagaaggcg gctctgagcg gatgggaggc cagcggagtg at
//
LOCUS       DASPUL010000097         5543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1522426, whole genome shotgun sequence.
ACCESSION   DASPUL010000097 DASPUL010000000
VERSION     DASPUL010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522426"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..4238
                     /locus_tag="VEJ00_02250"
     CDS             189..4238
                     /locus_tag="VEJ00_02250"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HXX00008.1"
                     /translation="MGAVGVALLAGLWYTTTDSFQAYARAKIVEELERITGGRAEIGS
                     FHVVPFHTQIEVRDITVHGSESPSDVPLAHADHLVAKMKVTSFLRAEFGFHSLTLEHP
                     VVHVAIAPDGSTNIPAPKVSRPASGESPVEQLFALSIDHLSARNGELYWADRKIPVEF
                     SAQETNLQMDYSFLRGHYESHLALGKVDTTLADFRPFSWMAKADLSLGPTFAEIQSLQ
                     WNSGHSHVEAHGRVSDFRNPHLDMTYDARADLGEAAAIARRHDLREGVAEFKGSGNWS
                     VDQFATSGSVSLRDLGWQDDQVSLKKVSASSDYSLTDQQFKLSKLQGKLLGGSLTGEA
                     QIDNWLHGIPPPPPGRTGKKAGENLPVITAARPPAKKGEKVKAPGVQTGVVHLRVRDV
                     SAAETASALDMPAHPLHNFHPAGLASGTVDAAWKGSPKDAEIAFAFDVTPPERPSSGE
                     LPMTARIQGKYRAGRDELELSQFNLSTPASRIQATGTLAASSTLHLSVSTSNLEEWRP
                     LVAALGGPTNVPFRVDGNATFNGVVGGSVSAPTLSGTLVAEDFEFALPATSRTPAQSV
                     HWDSLAVNMQFSPHQLTVRGGSLRHGDTSADFDVSAMLQNGHFTEDSPYTARVNLHNV
                     DVASTAALAGFDYPVTGTADVSLQISGTRANPQVQGHIRAMGASAYGEPIESFDADLR
                     MAGGETALNNVRLTHQNATVEGSAGYTPSTRGFRLDLTGKDFDLSQIRQIHLDRLPVQ
                     GSADFTLKGSGTVDAPVLNADVHIRDLTLDRELSGSLELVGETKDGQLRVQGHSEFPH
                     GAFTLDGSVGMHGDYPANLTVRMDHLDLDALWRSYLGGQLTGHSAVGGIVTMQGPLRH
                     PDLWMLNGDLNDVFLDVEYAKLHNQGPVRFNYRRQTVRIDPAHLVGDATDITGGGSIG
                     FAGSRDLDLTAEGHADLKLLHGVDPDLTASGIVSMNLTVAGTLKEPYVQGRLQVKDAS
                     ANYAGLPSGLSEMNGTLTFSRDHIHIEQMSARTGGGTLDFKGDGTTYNGQFNFDLTAV
                     GKDVRLRYPPGVSSTANAELRWVGTRSSSTVSGNILVTKLAVMPGFDFGSYLERSRQS
                     AAVTAANSPLYNVKLDIAVRTAPELQMKTAVARLSGDANLRLRGSAARPSVLGRAEIV
                     EGDATFNGIKFRLERGDITFANPVAIEPQVNLQATTHVRNYDLDVTVTGTPDRLNVNY
                     RSEPPLPKSDIIALLALGRTTQESEQLQQQSGQGLFSDEATALIINQAINSTVTSRMQ
                     KLFGVSRIKIDPQGLATETNPTARGPQVTVEQQFANNFTLTYSTNVSQTAEQIIQGEY
                     YFTRNVSVVGTRDQNGVVSFDVRIRRRKK"
     gene            4210..4932
                     /locus_tag="VEJ00_02255"
     CDS             4210..4932
                     /locus_tag="VEJ00_02255"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXX00009.1"
                     /translation="MSGFAAGRNRIDHSEGVRSAKPMTSSAEAATFAVLRLRMVEYQL
                     RARGISDGRVLDAMSRVPRHEFTPERYRDQAYEDHPLPIAEGQTISQPYIVASMLEAL
                     SIAPEDRVLEVGTGSGYVTALLAELASHVVSVERHAVLAEAARNLLARLGYRNLEVIV
                     GDGSQGLVENAPYDAILVSAAAAEVPQPLVVQLAEGGRMIIPVGPADCQQLQLVQNQN
                     GKLRTTLREPCRFVPLISGDDD"
     gene            5025..>5543
                     /locus_tag="VEJ00_02260"
     CDS             5025..>5543
                     /locus_tag="VEJ00_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HXX00010.1"
                     /translation="MIPDRDYNRSVPILHNLRLTLEMIKWEHSIFALPFALCGAMLAA
                     GGWPSGHQLAWIVVAMVAARSAAMAFNRLADASIDAANPRTRTRALPAGHLTLAFVAT
                     FVMVSSTLFVVAAAQLNRLALWLSPLALAVLLLYSYTKRFTRWSHLVLGFALGIAPAA
                     AWIAVRGSLDPRI"
BASE COUNT         1039 a         1757 c         1693 g         1054 t
ORIGIN      
        1 gctgacgtct cggccaagat ccgcgaattg ctgacgcagc agagagtcaa cgaactgctg
       61 gtctcctggc tgcagaccct gcgttcggag agcagggtga gcgttcccgg cacaacccag
      121 ggagtagaga cgcagtgacc gagcctgccc cccggcctcg gagcaggtgg aagttttggc
      181 tgatcgcgat gggggcggtc ggcgttgctc tgctggcagg gctctggtat accaccacag
      241 attcctttca agcctacgcc cgggcgaaga ttgtcgaaga gttggagcga atcacaggtg
      301 gccgcgctga aatcggcagc ttccacgtcg ttcctttcca cacccagatc gaagtgcgcg
      361 acatcaccgt gcatggcagc gagtctccgt ccgacgttcc gttggcccac gccgatcatc
      421 tcgtggcaaa gatgaaggtg acctcgtttc tgcgcgccga gttcggattc cactcgctga
      481 ccctcgaaca tccggtggta cacgttgcca tcgctccgga tggctcgacc aacattccgg
      541 ccccgaaggt ctcgaggcca gcttcggggg aaagccccgt agagcaactc ttcgcgctct
      601 ccattgatca cctctcggct cgtaatggcg agctttactg ggcggatcgc aagattcccg
      661 tggagttttc cgcgcaagag acgaacttgc agatggacta ttcgttcctg cgcggacact
      721 atgagagcca cctggcgctg ggcaaggtcg ataccacgct ggccgatttt cgcccgtttt
      781 cctggatggc caaggccgac ctcagcctgg ggccgacctt cgctgaaatc cagtcactgc
      841 agtggaactc cgggcactcg cacgtcgagg cgcacggacg ggtcagcgac ttccgcaatc
      901 ctcatctcga tatgacctat gacgcgcgtg ccgacctggg tgaagccgcc gcgattgcgc
      961 gccgccatga tctgcgagaa ggcgtggccg agttcaaggg aagcggcaac tggtcggtcg
     1021 atcagttcgc gacttctggc agtgtgtccc tgcgcgacct gggctggcag gacgatcagg
     1081 tctccctcaa gaaggtttcc gccagctccg actactcgct gaccgatcag caattcaagc
     1141 tttccaagct gcaaggaaaa cttctcggcg ggagtcttac gggggaagcg cagatcgata
     1201 actggctgca cggcattccg cctccgccgc cgggcaggac cggcaagaag gcgggagaaa
     1261 acctgccagt cattaccgcc gcacggcctc cagctaagaa aggcgagaag gtcaaggcac
     1321 cgggagttca gaccggcgtc gtgcatctgc gagtgcgcga tgtctccgct gctgagacgg
     1381 cctcggccct ggatatgccc gcgcacccgc tgcacaattt ccatcccgct ggcttggcct
     1441 cgggcaccgt cgacgcggcc tggaaaggtt ccccgaagga tgccgagatc gcatttgcat
     1501 tcgacgtgac tccgcccgag cgtccctcgt cgggtgaatt gcccatgacg gcccgcattc
     1561 agggcaaata tcgtgccgga agagacgagc tcgagctgtc tcaattcaac ctgtcgacgc
     1621 cagcctcgcg gatccaggcc accggaacgc tggctgcttc ctcgactctg catctttccg
     1681 tctcaacctc gaacctcgag gagtggcggc cgttggtcgc tgcgctcggc gggccgacaa
     1741 atgtgccatt ccgagttgat ggcaacgcca cattcaacgg cgttgtcggc gggtcagttt
     1801 cggctccaac cctcagcggt accctggtgg cggaagattt tgagttcgct ctcccggcca
     1861 cgtcgcgcac gccggcgcag tctgttcatt gggactccct ggccgtcaac atgcaatttt
     1921 ctccgcacca gctcacggtg cgcgggggta gtctgcgtca tggggacacg agcgccgact
     1981 tcgacgtcag cgccatgttg caaaacgggc atttcacgga agacagccca tatacggcac
     2041 gcgtgaatct gcacaacgtc gacgtcgcct cgaccgcggc gctggcgggc ttcgactatc
     2101 cggtgacggg aacggccgac gtctcgctgc aaatctccgg aacccgcgcc aatcctcagg
     2161 tgcaggggca cattcgcgcg atgggcgctt cggcatatgg ggagcccatt gagagcttcg
     2221 atgccgatct gcgcatggct ggtggggaaa cggcgctcaa caacgttcgc ctcactcacc
     2281 agaacgcgac ggtggaagga agcgccggct atacgccttc gactcgcggc tttcggctcg
     2341 acttgacagg caaggatttc gatctctcac agattcgaca gatacatctt gaccgcctgc
     2401 ccgtgcaagg ctccgccgac ttcaccctga aaggttcagg cacagtggac gcgcccgtac
     2461 tcaacgccga cgttcacatc cgcgacctca cgctcgatcg cgaactctcc ggcagcctgg
     2521 agttggttgg ggaaacaaaa gacggtcaac tacgagttca ggggcattcc gagttcccgc
     2581 acggtgcgtt cactctcgac gggagcgtcg ggatgcacgg cgactatccg gcgaatctta
     2641 cagtccgtat ggaccacctg gatctcgacg ccctgtggcg ctcctatctg ggtgggcaac
     2701 tcacggggca ctcggcagtc ggcggcatcg tgaccatgca aggcccgctg cgtcaccccg
     2761 atctgtggat gctgaacggc gacttgaacg acgtcttcct ggatgtcgaa tacgcgaagc
     2821 ttcacaacca gggaccagtg cgcttcaact acaggcggca aacggttcgc atcgacccgg
     2881 cccatctggt gggcgacgcg accgacataa ccggaggagg ttcaatcggc tttgcgggat
     2941 cgcgcgatct cgacctcacc gccgagggac acgccgatct caagttgctg catggggtgg
     3001 atccggatct gactgcctcc ggtatcgtat ccatgaacct gaccgtagcg ggcacgctca
     3061 aagagccgta cgttcaggga cgtctccagg tcaaggacgc ctccgccaat tacgccggcc
     3121 tgcccagcgg gctcagcgaa atgaacggca cgctgacctt ttcccgcgac cacatccaca
     3181 ttgagcagat gtcggcccgc accggcggcg gaacgctcga tttcaaaggt gacggcacca
     3241 cctacaacgg ccagttcaat ttcgacctga cggcggtggg aaaggacgtt cgcctgcgct
     3301 atccgcctgg agtcagctcc acggcaaacg cggagttgcg ctgggtcgga acgcgctcgt
     3361 catcaacagt atccggcaat attctggtga caaagctcgc ggtcatgccc ggtttcgatt
     3421 tcggctcgta cctggagcga agccggcagt ccgccgcggt caccgcggcc aattctccgc
     3481 tgtacaacgt gaagctcgac atcgccgtcc gcaccgcgcc ggagttgcaa atgaagaccg
     3541 ccgtagcccg gctctcgggc gatgccaacc tgcgcctccg cggctcggcg gcacgcccca
     3601 gcgttcttgg ccgcgccgaa atcgtggaag gcgatgcgac cttcaacggc atcaaattcc
     3661 ggctggagcg cggcgacatc accttcgcca atcccgtcgc catcgaacct caggtgaacc
     3721 tgcaggccac aacccacgtg cgcaactacg atctcgacgt caccgtcact ggaaccccgg
     3781 accgcctcaa cgtgaactac cgctcggaac ctcctctgcc caagtccgac atcattgcgc
     3841 tgttggcgct cggccggacc acccaggagt cagagcaatt gcagcagcag tccgggcaag
     3901 gcctgttcag cgatgaggcc acggccctga tcatcaacca ggccatcaac tcgacagtga
     3961 ccagcaggat gcagaagcta tttggagtga gccgcatcaa gatcgatcca cagggcctgg
     4021 ccacggagac caaccccacc gcccgcggtc cgcaggtcac cgttgagcag cagtttgcca
     4081 acaacttcac gctcacctac agcaccaacg tttcgcagac tgcggaacag atcattcagg
     4141 gcgagtacta tttcactcgc aatgtctcgg tggtgggcac gcgcgaccag aacggcgtgg
     4201 tcagttttga tgtccggatt cgccgccgga agaaatagaa ttgaccacag cgagggcgtg
     4261 cgctccgcca agccgatgac ttcctctgcc gaagctgcca ctttcgcggt cctgcggttg
     4321 cgcatggtcg aataccagct gcgcgcgcgg gggatcagcg acgggcgcgt gctggatgcg
     4381 atgtcccgtg ttcccaggca cgagttcacc ccggagcgct atcgggatca ggcctacgaa
     4441 gatcaccctc tgccgatcgc cgaaggtcag accatctctc agccctacat tgtggcctcg
     4501 atgctggagg cattgtcgat tgcgccggag gaccgtgtgc ttgaggtggg gacgggatca
     4561 ggctacgtga ccgcgttgct ggccgaactg gcgtcacacg tggtctcggt cgagcgccac
     4621 gcagtgttgg cagaggcggc gagaaacctg cttgcgcgcc tgggctaccg aaacctggaa
     4681 gtgattgtgg gcgacggcag ccagggcctc gtggagaatg cgccgtacga cgcgattctg
     4741 gtctcggcag cggcggccga ggtgccgcaa ccgttggtgg tgcaattggc ggagggcggc
     4801 cggatgatca ttccggttgg ccctgcggac tgccagcagt tgcagctggt gcaaaatcag
     4861 aacggcaagc tgcggacaac cctgcgcgag ccctgccgct tcgtgccgct gatttcgggc
     4921 gatgacgact aggagcagtt tcctttgtgg tgctctgtgg ctgcctggtt cgaggtttgc
     4981 gacttaacca ttgagggcac agggatccca gaggacgcaa gacggtgatt cctgatcgcg
     5041 attacaatcg atcggtgccc atcctgcaca acctgcgcct gacactggaa atgatcaagt
     5101 gggaacactc gatttttgcg ctcccgtttg ccttgtgcgg ggccatgctg gcggcgggag
     5161 gatggcccag cgggcatcaa ctggcgtgga tcgtggtcgc gatggtcgcg gcgcggtctg
     5221 cggcgatggc gtttaatcgc ctggcggacg cctcgatcga cgctgctaac cctcgtacgc
     5281 ggacgcgggc gctgccggcg ggtcatctta cgctggcgtt tgtagcgact ttcgtcatgg
     5341 tttcgagcac actctttgtg gttgccgcag cgcagctgaa ccgcctcgct ctgtggcttt
     5401 ctccgctcgc tctggctgtc ctgctgcttt actcctacac caagcgcttc acgcgttggt
     5461 cgcatctcgt gctgggtttt gcgctcggaa ttgcacccgc agcggcctgg atcgctgtgc
     5521 gcggctcgct cgacccgcgc atc
//
LOCUS       DASPUL010000098         5875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_152003, whole genome shotgun sequence.
ACCESSION   DASPUL010000098 DASPUL010000000
VERSION     DASPUL010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152003"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..691
                     /locus_tag="VEJ00_02265"
     CDS             86..691
                     /locus_tag="VEJ00_02265"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX00011.1"
                     /translation="MRYAFLVETYATERVKVVSVWSEFRDEDLPFRPHAGDPRGRSVH
                     EQMVHQCVSEDLWLRNMLGIDVGVPPLPKTETRVEFMKRYAEDSGKRLAALRAKEEDW
                     WEGVTNFFDVKRSRTWVMVRRMTHTSHHRGQLMAMLRMLGREIHSNYGPTADTGGLMQ
                     NHAPTIYAYSSLDEMLAGESAGGSKARLPGAGGKAVTERPG"
     gene            complement(766..3282)
                     /locus_tag="VEJ00_02270"
     CDS             complement(766..3282)
                     /locus_tag="VEJ00_02270"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HXX00012.1"
                     /translation="MPVSKTSKSATTAVMESSNNGSNPERERVFSAYRQWGYLEGDLD
                     PLGFLRPRPTPELERDGKYARQAHAIYSSTIGVEINHIYAPERRRWIYDRMESPDQGY
                     EVTEENQKRILDLLIRADVFEQVMQQRYLGSKRFSLEGVTALIPLADEVLDTAARLGA
                     IELVMGMSHRGRLNVIAHVARRPPEEIFAGFEDVDPRSVLGSGDVKYHMGATGEYVTR
                     SGGKIHIHLVSNPSHLEAVDPVTVGRTRAKQDRTGEGGREKYLPLLVHGDAAFAGQGI
                     LAETMNYADLPGYTVGGTIHVVVNNLLGFTTSYTEEHSTRFAACIARRQSIPIFHVNG
                     EDVDAVVRVARMATEYRYRFGTDVVIDLIGYRRHGHSEVDDPTITQPLLYKKIKEHPP
                     LWEIYADDIGAADAQAQADAIKAEFEAVQKNAGSIKQKPTLRELPRYWDNYFGGLYKP
                     EFEVETGLTSAEIGELTERLTTYPDGFHIHPKVKKLLEQRAEMGAGKRLVDYGMAEAL
                     AFASLVKSGIHVRFSGQDSRRGTFNQRHSALVDIENENEYVPLENIAPGQARCGIYNS
                     TLSEAGVLGFEYGYSRDYPESLVLWEAQFGDFANVAQAIVDQFIAAGEAKWNLLSGLV
                     MLLPHGYEGQGPEHSSARIERFIQLAAKHNMQIAQPSNAAQYFHLLRRQALRHWRKPL
                     IVFTPKSMLRHPDASSPIEDFTHKNFLNVIPDTEVGEADRILICTGKIGHELQAERKR
                     RKDTNTAIVFLEQLYPFPEQEVVAEFDRHGRHADIVWVQEEPANMGALFNMLTRLKRI
                     AGERHVLSVKRSSSPSPATGSAKAHEVEQKTLLTLAFTTK"
     gene            3461..4252
                     /gene="speB"
                     /locus_tag="VEJ00_02275"
     CDS             3461..4252
                     /gene="speB"
                     /locus_tag="VEJ00_02275"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HXX00013.1"
                     /translation="MPTLVGVPFDANSSYLRGTGDAPPAIRRELHCDAYNRWTELGVD
                     LGAAGAYEDAGDLALTERDAFPVIETGVAKLLEQGRRPVSLGGDHSITFPLVKAFAGR
                     YPELAIIHFDAHPDLYDELDGNRLSHACPFARIMESRLAKRLVQVGVRTINGHQREQA
                     RRFGVEIIEMPALPAYDKLKADGPVYVSFDLDVLDPAFVPGVSHREPGGMTVREAIAH
                     LHAIKGEIVGADLVEYNPVRDLGGVTATVAAKILKEILGKMIAEG"
     gene            complement(4290..4763)
                     /locus_tag="VEJ00_02280"
     CDS             complement(4290..4763)
                     /locus_tag="VEJ00_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HXX00014.1"
                     /translation="MPLVVRPSPIHAVGVYTTTPIRKGARVVEYTGSRITPEEADRRY
                     DGHSRTYLYGLEDGKTVIDGEGLGAYLNHSCDPNCEVDEIKGRVWLFALRDIAAGEEL
                     LWDYNLYDDEAPAPCYCGSRKCRGTMYSKEWIAKMRRRETCRRRSRSADTSRAGA"
     gene            complement(4866..5498)
                     /locus_tag="VEJ00_02285"
     CDS             complement(4866..5498)
                     /locus_tag="VEJ00_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00015.1"
                     /translation="MLSAAVFLCACLAYGQDQDAPSLGDAARQNRLQKQQKDAQAKDA
                     PTKSAATGSPTKDVQDQDNEAQSKDAPSKDAADKSTPKTPHVITNDEIPEHTGAGAPS
                     TSGYQNPGVQYPSNSGSAEAAGEQWKSAIQGQKNAISSLQSQINSLQDSIKFAPGNCV
                     SGCVQWNERQRQKQEQVESMKSQLEQMQKQLEDMQEACRKQGFGSSVYDP"
     gene            complement(5639..>5875)
                     /locus_tag="VEJ00_02290"
     CDS             complement(5639..>5875)
                     /locus_tag="VEJ00_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HXX00016.1"
                     /translation="GKPYLAVPVRHQFEQIFNAYWLEKSGYGAYWDELNKERIEAFLY
                     NLPHYCEALAKYPRQGNGALFAKLDDLIAGYSLT"
BASE COUNT         1067 a         1637 c         1952 g         1219 t
ORIGIN      
        1 aaagtgccgc cagtgtcgat ggcaatgcgg agcggttggt cacggcgggg catgggcgcg
       61 gagaactagg gtaacatctg cggctatgag atacgcattc ctggtcgaaa cttacgcgac
      121 cgagcgcgtg aaggtggtga gcgtgtggag cgagtttcgg gacgaagacc taccgttccg
      181 cccgcacgca ggcgatccgc gcggacgcag cgtgcatgag cagatggtgc atcagtgcgt
      241 cagcgaggat ttgtggctca ggaacatgct gggaattgac gtgggagttc ctccgttgcc
      301 gaagacggaa acgcgtgtgg aattcatgaa gcgctacgcg gaggacagcg gcaaacggct
      361 ggccgcactg cgagcgaagg aggaagactg gtgggaaggc gtgaccaatt tctttgacgt
      421 gaagcgatcg cggacctggg taatggtgcg gcgcatgaca catacctcgc atcatcgcgg
      481 gcaattgatg gcgatgctgc gcatgctggg gcgggagatc catagcaact acgggccgac
      541 cgcggatacg ggcgggttga tgcagaatca tgcgccaacg atttatgcat attcgagctt
      601 ggacgagatg ctggccggag agagcgcggg cgggagcaag gcacgactgc cgggagcggg
      661 cgggaaagcg gtcacggaga ggccggggta gagccccact tctcgcgcaa agaacgcgcg
      721 agaaatgggg cacccgcaaa gtgcgcggcc ctattccaga aagttctact tcgtcgtgaa
      781 cgccagggtc agcaaggtct tctgttccac ctcgtgcgct ttggcgcttc ccgtcgccgg
      841 gctgggactc gagctgcgct tcacgctgag cacgtggcgc tctccggcga tgcgtttcaa
      901 gcgcgtcagc atgttgaaaa gcgcgcccat gttggcgggc tcttcctgca cccagacgat
      961 atcggcgtgc cggccgtggc gatcgaattc ggccacgact tcctgctcgg ggaagggata
     1021 gagttgttcc agaaatacaa tcgcggtgtt tgtgtccttt cttcttttcc gttcggcctg
     1081 gagctcgtga ccaatctttc ctgtgcagat caggatgcgg tcggcttcgc cgacttcggt
     1141 gtcagggatg acgttgagaa aattcttgtg ggtgaagtct tcaatcggtg aactcgcatc
     1201 gggatgtcgc agcatgctct tcggcgtgaa gacgatcagc ggcttgcgcc agtgccgcag
     1261 cgcctggcgg cgcagcaggt ggaagtactg agccgcgttc gacggctgcg cgatctgcat
     1321 gttgtgtttc gcggcgagct gaatgaagcg ctcgatgcgg gcgctggagt gctcgggtcc
     1381 ctggccttcg tagccgtgcg ggagcagcat cacgaggccg gagagcaggt tccacttggc
     1441 ttcgccggcg gcgatgaact ggtcaacaat agcctgtgcc acgttggcaa agtcaccgaa
     1501 ctgggcctcc caaaggacga gcgattcggg atagtcgcgg ctgtagccgt attcgaagcc
     1561 gagaactccg gcttcggaga gcgtggaatt gtagattcca caccgcgcct gaccgggcgc
     1621 gatgttttcg agcgggacgt attcattctc gttttcgatg tcgaccagag cggaatggcg
     1681 ctggttgaag gtgccgcggc gggagtcctg gccgctgaag cgcacgtgga tgccggactt
     1741 caccaggctg gcgaaagcaa gggcttcggc catgccgtag tcgacgaggc gcttgcccgc
     1801 gcccatttca gcgcgctgtt cgagcagctt cttgactttc gggtggatgt ggaaaccgtc
     1861 gggataggtc gtgaggcgtt cggtgagttc gccgatttct gcggacgtga ggccggtctc
     1921 gacttcgaat tccggcttgt acaggccgcc gaaatagttg tcccagtacc tgggaagttc
     1981 gcgcagcgta ggcttctgct tgatgctacc ggcgtttttc tgcaccgctt cgaactcggc
     2041 cttgatggca tcggcctgag cctgggcgtc ggctgcgccg atgtcgtcgg cgtagatttc
     2101 ccatagcggc ggatgctcct tgattttctt gtagagcaac ggctgcgtga ttgtcgggtc
     2161 atcgacttcg ctgtgcccgt ggcggcggta gccgatcaag tcgatgacga cgtcggtccc
     2221 aaatctgtag cgatattcgg tggccatgcg ggcaacgcga acgacggcat cgacgtcttc
     2281 cccgttgacg tgaaagatgg gaatcgactg ccggcgtgca atgcaggcgg cgaagcgcgt
     2341 ggaatgctct tcggtgtagc tggtggtgaa gccgagcagg ttgtttacga cgacgtgaat
     2401 tgtgccgccg acggtgtagc cggggagatc ggcgtagttc atggtttcgg caagaattcc
     2461 ctgcccggcg aacgcggcgt cgccgtgcac cagcaacggg aggtattttt cgcgtccgcc
     2521 ttcgccggtg cggtcctgct tggcgcgggt gcggccgacg gtgacggggt cgacggcttc
     2581 caggtggctg ggattcgaga ccaggtggat atggattttg ccgccgctgc gggtgacgta
     2641 ttccccggtc gcgcccatgt gatacttcac atcgccggag ccgagcacgc tgcgcggatc
     2701 aacgtcttcg aatcccgcaa aaatctcttc gggtgggcgc cgggcaacgt gggcgatgac
     2761 gttaaggcgc ccgcgatggc tcatgcccat cacgagctcg attgccccca ggcgcgcggc
     2821 cgtgtccaga acctcatccg ccaacgggat cagtgcggtc acgccttcga gcgagaagcg
     2881 cttgctgccc agatagcgct gctgcatcac ctgctcgaac acgtcggcgc gaatgagcag
     2941 atcgaggatg cgcttttgat tttcttccgt gacctcgtaa ccctgatcgg gcgattccat
     3001 gcggtcgtag atccagcggc ggcgctcggg agcgtagatg tggttgattt cgacgccgat
     3061 cgtgctggag tagatggcgt gggcctggcg ggcgtacttg ccgtcgcgct caagttcggg
     3121 tgtaggccga gggcgcagga agccgagagg atcgaggtcg ccctcgaggt atccccattg
     3181 gcgataggcg ctgaagacgc gctcacgctc ggggttggag ccgttgttcg acgattccat
     3241 caccgccgta gtggcggact tgctggtttt cgagacaggc ataagaatgc agttcctagg
     3301 ataacggatg cgggcggagg cgtgcgagat tcgaccgtga gacgaggaac tcgtaagtcg
     3361 caaatggacc caactgcagg cgtgtgtgca ccacagccgt ttgcaggata gacttcgtta
     3421 gattcgatcc cggaaaccat tgcttatgaa gaaacttggc atgccaactt tagtgggagt
     3481 tccgtttgac gcgaactctt cttacttgcg cgggacaggg gatgctcccc ccgcaattcg
     3541 ccgtgagttg cattgcgatg cctacaaccg gtggacggag cttggcgtgg atcttggcgc
     3601 cgccggcgcc tatgaagatg caggcgacct tgccctgact gagcgcgacg cattcccggt
     3661 gatcgaaacc ggcgtggcga agctgctgga gcaagggagg cgtccggttt cgctgggagg
     3721 ggatcactcg atcacgtttc cgctggtgaa ggcgtttgcg ggacggtatc ccgagctggc
     3781 catcattcac ttcgacgcgc atccggacct gtacgacgaa ctcgatggca accggctgtc
     3841 ccacgcctgc cccttcgccc gcatcatgga gtcgcgtctg gccaagcggc tggtgcaggt
     3901 gggcgttcga accatcaacg gacatcagcg cgagcaagcc aggagattcg gggtcgagat
     3961 catcgagatg cctgcgctgc cggcctacga caagctgaag gcagacgggc cggtgtacgt
     4021 ttcgttcgat ctggacgtgc tcgacccggc atttgtgccg ggagtttcgc accgcgagcc
     4081 tgggggaatg accgtgcgcg aagcgattgc gcacttgcat gcgatcaagg gggaaatcgt
     4141 aggagcggat ctggtcgaat acaacccggt gcgggacctg ggcggcgtga ctgcgacggt
     4201 ggcagcaaag atcctgaagg aaattctggg gaagatgatc gcggaaggtt agcggcacgg
     4261 accgggaaga aggcagcgga gcaagccgct caggctccgg ctctggaggt gtccgctgat
     4321 cttgatctcc gtctgcaggt ttcgcggcgg cgcatcttgg cgatccactc tttcgagtac
     4381 atagttccgc ggcacttgcg cgaaccgcag tagcagggag cgggagcttc gtcgtcgtac
     4441 aggttgtaat cccataggag ctcttccccg gcggcgatat ctcgcagcgc aaagagccag
     4501 acccggcctt tgatctcatc cacttcacag ttgggatcgc aggagtggtt cagataggcg
     4561 cccaggccct cgccgtcgat cacggtcttg ccatcctcca gcccgtacag gtaagtacgc
     4621 gagtggccgt cgtagcggcg atcggcttct tcaggagtaa tgcgcgagcc ggtgtactca
     4681 acgacacgcg cccctttgcg gatcggggtc gtcgtgtaga cgccgacggc atgaatgggc
     4741 gaaggacgga ctacgagggg caccgttcta atctcgcaca atctgcggct tgggaaaacc
     4801 cgaggcaaaa ccagcgccgg acgctgggtt tggggcgcaa cgtgtgagcg cgatgcaggc
     4861 ccgcgctacg gatcgtaaac cgagctgcca aagccttgct tgcggcacgc ttcctgcatg
     4921 tcttccagct gtttctgcat ctgctccagt tgagacttca tgctttcgac ctgttcctgc
     4981 ttctgacgct ggcgctcgtt ccactgcacg cagccggaaa cgcagttgcc gggagcaaac
     5041 ttgatggagt cctgcaggct gttgatctgg ctctgcaggg aggagatagc attcttctgc
     5101 ccctggatcg cgctcttcca ctgttcgcct gccgcttcgg cgcttccgga gttcgacgga
     5161 tactgcacgc ctgggttctg atagccggaa gtggagggcg ctccggctcc ggtatgctct
     5221 ggaatctcgt cgttggtgat cacatgcggc gtcttaggcg tgctcttgtc ggctgcgtcc
     5281 ttgctcggcg catccttgct ctgggcctca ttgtcttggt cttgcacgtc tttggtcgga
     5341 ctccccgtcg ccgcgctctt cgtcggcgca tctttcgcct gggcatcctt ctgctgcttt
     5401 tgcaggcgat tctgccgggc cgcgtcgccg agagacggcg cgtcctgatc ctggccgtag
     5461 gccaggcaag cgcaaagaaa aacggcagct gaaagcacga cgagacttct gcacatcgac
     5521 cgcatacgtg aaacctcccg gagccgtccg cacagggact ggcgacgccc cgcaagcatg
     5581 gtatcgcttg cggatgtacg gtcggaggtt acgtcagtac acgcgctcac gggttcatct
     5641 atgtgaggct atacccggcg atcaggtcgt caagcttcgc aaacagggcg ccgttgccct
     5701 gtcggggata ttttgccaga gcttcgcaat agtgcggcag attgtagaga aaagcctcga
     5761 tccgctcctt gtttagctcg tcccagtaag ccccatagcc ggacttctca agccagtaag
     5821 cattgaagat ctgctcgaac tgatggcgta ccggcaccgc cagatacggt ttgcc
//
LOCUS       DASPUL010000099         4811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_699887, whole genome shotgun sequence.
ACCESSION   DASPUL010000099 DASPUL010000000
VERSION     DASPUL010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699887"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="VEJ00_02295"
     CDS             <1..978
                     /locus_tag="VEJ00_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00017.1"
                     /translation="RIDAQQQEINRLSALVEKLQAALEPIASTQPVLPRVSSALLAAS
                     ALPAGSPAPSPTPAPAPVVATSSTPRELLPDIGHIGAELGFFVGGASNPYKDNNGFAT
                     GGFIDLPWKNVPAGKLSYEIMIGLQRSTTSQQTTSGVNVLVNSVLNSYLGNTAANNTS
                     LPQFLGATSTGMLPITSTVQERSKVLTVAPFELKYAITKLGKFRPYAVVGLGAYVWIG
                     SDNNSQSFNAVTSLNNFSAGLGTTYGALVNSLLQGSQIGGLVPTAPQLAARGVAHGQG
                     NLLFGGQVGGGFEYRLTPKLSWGADFRRNQVEGSFASFNTFTFKQGFHW"
     gene            996..1550
                     /locus_tag="VEJ00_02300"
     CDS             996..1550
                     /locus_tag="VEJ00_02300"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXX00018.1"
                     /translation="MSTKNDKDNPKRFSRRQALQAGGLSLAAGIVASAAPAVAAEARF
                     AGDLAILNVALGLEHQAIAAYDAGAKSKLLSADQLRIAVSFQTDHKRHRDALTHFIKR
                     FGGTPVAPRSSYDFGTITSAADIVKLAQSLEDGAEAAYLANAGKLENREILNAAVPIL
                     EDEVRHNTVFKQLLGMDVTERLKY"
     gene            1574..2137
                     /locus_tag="VEJ00_02305"
     CDS             1574..2137
                     /locus_tag="VEJ00_02305"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXX00019.1"
                     /translation="MNVEKPELNSVVPVLEERNVRREWLRLASTASAGAILFLLTGNP
                     KNLYGQSLEGDLGILTAALYLEQEAITAYQAGAESKLLSPGVLKAALAFQSDHKYHRD
                     GISGVLKSLGVEPKGPENKYDFGTLHSEKDILRLARDREHGAVLAYGTLASNILTKAV
                     LNFGANVLVDEVRHRTILNAALGIPNY"
     gene            2223..2417
                     /locus_tag="VEJ00_02310"
     CDS             2223..2417
                     /locus_tag="VEJ00_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00020.1"
                     /translation="MSTMSITSNRSGKKLAPLTYFLGFIFVLFIGILVFCYAVTRRTT
                     LIYLDEHGKPVAASSEHSHH"
     gene            2419..>4811
                     /locus_tag="VEJ00_02315"
     CDS             2419..>4811
                     /locus_tag="VEJ00_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXX00021.1"
                     /translation="MSAGRECDLCGLGCGKHPLRQSVGSADRFFCCSGCMNVYLILWE
                     SGAIKAGQDIRQTELFQRSLQLGLISNRSSEEASSDSVEPAPGTATEELLLHVTGMWC
                     TSCAWLIERALASVPGVTKAEASFASDLVKVQYLPQTTPPERIVARIGSLGYKAQKYV
                     DENDADNAEQRDLIIRTGLAAFLWANVMSFSLVLYAGYFEQISGSVRRGLPFLLMALA
                     TPVVFYCAQPVLGIAWRGLRNRTIRMEALLSLGILAAYGYSVVQTFRGQIHLYFDTAT
                     VIVTLVLAGKLIERGAKEKVSRWISMLHRMMPNKVRLRVQGSERFVNVDALEPGELFV
                     VKAGERIPSDGIVAEGNSHADESLLTGESAPVAKSPGDSVAAGSINLDGVLTARATTS
                     ARDSTLSRIIARVEQALSSRCEIERAVDHVSRVFVPVVVVIAILTFIVTWITGSASLA
                     QSLMRAVTVLVIACPCALGLATPLAITAALGSASRQGILVRDSRVVETLRKIDTVILD
                     KTGTVTEGKFSLLELMPCRHRQREAVFAGQAARSGAGISESPASEGSAGALDEALSLL
                     ASLERYSEHPLGAAVAEFARREGATLVDAASVEIHKGRGITGIVEGKHIFVGNRRLAK
                     ELAVLIDESIENQAARWESEGKTVAFAGWDGEIRALAAFGDRVKPEAFELVAELRRRG
                     IETRLISGDAQATTHWVAASLGVDSCEAEVLPENKTEAVRRLQASGAIVAMVGDGIND
                     APALAQADLGIAMGSGTDIAMKAAAVVLMGGSLHKILDVFDLSRKTIRVVRQNLFWA"
BASE COUNT          945 a         1473 c         1445 g          948 t
ORIGIN      
        1 cgcattgacg cccaacaaca ggagatcaac cggctgagcg cccttgtcga gaagctgcaa
       61 gccgcgctcg aaccgattgc ctcgactcaa cccgtgctcc ccagggtcag cagcgcattg
      121 ctggctgcga gcgctttgcc cgcgggttct cccgctccct cccccactcc cgctccagcg
      181 ccggtcgtgg ccaccagttc gactccacga gagctgctgc cggatatcgg ccacattggc
      241 gccgagctgg gcttttttgt gggtggcgcc agcaatccct acaaggacaa caatggcttc
      301 gccacaggtg gcttcatcga tctgccctgg aagaacgttc cggccggcaa gctgtcgtat
      361 gaaatcatga tcggcctgca acgctcgacc acgagccagc agacaacttc cggcgtgaac
      421 gtgctggtca attcagtgct gaactcctat ctggggaaca cagccgccaa caacacctcc
      481 cttccgcaat tcctgggggc tacttccacc ggcatgctgc ccatcaccag cacggtgcag
      541 gagcggtcga aggtcctgac cgtagctcca ttcgaactga aatatgcgat cacaaaactg
      601 gggaagttcc gcccttatgc cgttgtcggc ctgggggcct atgtgtggat tggctcggac
      661 aataacagcc agtcgttcaa tgcagtcact tcgctgaaca acttttcggc gggcctggga
      721 acaacctatg gcgccttggt gaattctctg cttcaaggct cgcagatcgg aggcctggtt
      781 ccgaccgcgc cacaactggc ggcgcgcggg gttgcgcatg gccagggcaa cctgctcttc
      841 gggggacagg tgggtggcgg ctttgagtat cgcctcacgc caaagctgtc gtggggagcc
      901 gatttccgcc gcaaccaggt ggaaggttcc ttcgccagct tcaacacctt caccttcaag
      961 caaggctttc actggtaagg aaccggaggc ggactatgtc gacaaagaat gacaaagaca
     1021 atccgaaacg gttttcacgg cggcaggcct tgcaggccgg cggactttca ctggcagcgg
     1081 gaatcgtggc gtcagccgca cccgcagtcg ccgccgaggc ccgcttcgcc ggcgatctgg
     1141 cgattctgaa tgtcgccctt ggcctggagc atcaggccat cgccgcttac gatgccggcg
     1201 ccaaaagtaa attgctgtct gccgatcaac tcaggatcgc agtcagcttt caaacggatc
     1261 acaaacgcca ccgcgacgcg ctgacgcatt tcattaaacg tttcggaggc acgccggtcg
     1321 cgcccaggag cagctatgac ttcggcacga tcacctcagc cgcagatatc gtcaagctcg
     1381 cacaaagcct ggaagacggc gccgaggccg cctatctcgc caacgcgggc aaactcgaga
     1441 accgcgaaat cctgaacgcc gctgttccca tccttgagga cgaagtgcgc cataacaccg
     1501 tcttcaaaca actgctcggc atggacgtga ccgagcggct gaagtactga agacggaaag
     1561 gaggaaccag tccatgaacg tcgaaaagcc ggaattgaac tccgtggtgc ccgtccttga
     1621 ggaaagaaat gtgcgtcgag agtggctccg tctggccagc acggcaagtg ccggagccat
     1681 cctctttctg ctgacgggta acccgaagaa cctgtatgga caatcgctgg aaggcgacct
     1741 gggaatcctc accgcggctc tctatttgga gcaggaagcc atcactgcgt accaagccgg
     1801 agccgaaagc aagctgctct ccccgggcgt gctgaaggcg gcgcttgcct tccaaagcga
     1861 tcacaaatat caccgggacg gaatcagcgg agttctgaag tcgctcggtg tggaacccaa
     1921 agggccggaa aacaagtatg acttcggaac tctgcacagc gagaaggaca ttcttcgtct
     1981 ggcccgggat cgggagcacg gcgccgtgct ggcttatggc acgctggcgt ccaacatcct
     2041 gaccaaggct gtactgaact tcggcgccaa tgtgctggtg gatgaagtcc gccaccgcac
     2101 gattttgaat gcggcgctgg gcataccgaa ctactgattg agaaacacgg aaggggccgg
     2161 acattccggc cccgctccga attccgggcc tgcagtcaat tggcccccaa tggagaggca
     2221 gcatgagcac catgtccatc acatccaatc gcagtggaaa gaagctcgcg ccactcacgt
     2281 attttctggg cttcatcttc gtgctcttca ttggcatctt ggtcttctgt tatgccgtga
     2341 ccaggcgtac gaccctcatc tacctcgacg agcacggcaa gcccgtggca gcgagttccg
     2401 agcactcgca tcactgaaat gagcgcgggc agagaatgcg acctgtgcgg cctgggatgc
     2461 ggcaaacatc ccttgcgcca gtctgtcggc tcggccgatc gcttcttctg ctgcagcggc
     2521 tgtatgaacg tgtatctgat cctgtgggag agcggggcca tcaaggccgg ccaggatatt
     2581 cgccagaccg agctcttcca gcgcagcctt cagctcgggc tgatctccaa ccgatcttct
     2641 gaagaagcgt cgtcagattc cgtcgagcct gctcctggta cggcgacaga ggaattgctc
     2701 ctccacgtga cggggatgtg gtgcacttct tgcgcgtggc tgatcgagcg cgcacttgct
     2761 tcggtcccgg gtgtcacaaa ggccgaggcc tctttcgcct ccgacctggt caaggtgcaa
     2821 taccttccgc agacgacgcc cccggaaaga atcgtggctc ggatcggcag tttagggtac
     2881 aaggctcaga agtatgtcga cgaaaacgat gccgacaatg ccgagcagcg cgacctgatc
     2941 attcgcaccg ggctggccgc ctttctgtgg gccaacgtga tgagcttcag tctcgttctc
     3001 tatgccgggt atttcgaaca gatttccggc agcgtgcgcc ggggcttgcc cttcctactc
     3061 atggccctgg ccactccggt ggtcttctat tgtgcgcagc ccgtcctcgg tatcgcctgg
     3121 cgaggcttgc gcaaccgcac catccgcatg gaagcgctgc tcagtctggg gattctcgcg
     3181 gcatacggtt acagcgtggt gcagacgttt cgcggccaga ttcatctgta tttcgatacc
     3241 gcaaccgtca tcgtgaccct ggtgctggca ggcaagctca tcgagcgcgg ggcgaaagag
     3301 aaagtcagcc gctggatttc gatgctccac cgcatgatgc ccaacaaggt gcgactgcgg
     3361 gtccagggct cggagcgttt tgtgaacgtt gacgcgctcg aaccggggga actgttcgtg
     3421 gtcaaggccg gcgagcgcat cccttccgac ggcatcgtgg cggaaggcaa ttcccacgca
     3481 gatgaatcgc tgctgacggg tgaatcggct cccgtcgcta agagccccgg agactccgtc
     3541 gccgcgggca gcatcaatct ggatggcgtc ctgactgcgc gcgccaccac gtccgctcgc
     3601 gactcgacct tgtcgcggat cattgcacgc gtcgagcagg cgctcagcag ccgttgcgaa
     3661 attgaacgtg ccgtcgacca tgtttcccgc gtttttgtcc ctgtcgtcgt cgtgatcgcc
     3721 attctgacct tcatcgtgac gtggataacc ggctcggcca gcctggctca gtcgctgatg
     3781 cgcgcggtca ccgttcttgt gattgcctgt ccctgtgcct tgggcctggc gactccgctg
     3841 gcgatcactg ccgccctggg ctcggcttcg cgccagggca ttctggttcg cgacagccga
     3901 gtcgtcgaga cgctacgaaa gatcgatacg gtcattctcg acaagaccgg taccgtcacc
     3961 gaaggaaaat tctcgcttct ggaacttatg ccttgcaggc accgacagcg cgaagccgtc
     4021 ttcgctgggc aagccgcgcg ctctggggca ggcatttccg agtcgccggc ttccgagggc
     4081 agtgccgggg cactcgacga agctctttca cttctggctt cgctcgagcg ctattccgag
     4141 catccgttgg gtgctgcggt cgcggagttc gctcggcggg aaggcgccac gctagtcgac
     4201 gcggcttcgg tcgagatcca taaagggcga ggcatcactg gcattgtcga ggggaaacac
     4261 atcttcgtcg gaaatcgccg gcttgcaaag gaattagcgg tcctgatcga cgaaagcatc
     4321 gagaaccagg ctgcccgctg ggagagtgaa ggcaagacag tcgcatttgc cggctgggac
     4381 ggcgaaattc gcgctctggc cgctttcggc gacagagtga agcctgaagc cttcgaactg
     4441 gtggcggagc tgcgacggcg tggcatcgaa acccggctca tctcgggaga cgcccaggcc
     4501 acgacgcatt gggtggctgc aagtcttggc gtcgattcct gcgaagccga agttcttcct
     4561 gagaacaaga ctgaagccgt ccgccgcctg caggcgagtg gcgcaatcgt ggctatggta
     4621 ggcgacggaa tcaatgacgc gcccgcgctc gcgcaggccg atctcggaat cgccatgggg
     4681 tctggcaccg atatcgccat gaaggccgcg gcggttgtgc tgatgggcgg ctctctgcac
     4741 aaaattcttg acgtcttcga cctctcgcgg aagacaatcc gggtggtccg ccagaatctc
     4801 ttctgggcgt t
//
LOCUS       DASPUL010000100         5051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_700234, whole genome shotgun sequence.
ACCESSION   DASPUL010000100 DASPUL010000000
VERSION     DASPUL010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700234"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VEJ00_02320"
     CDS             <1..258
                     /locus_tag="VEJ00_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HXX00022.1"
                     /translation="VFLDAPVEELWRRCCKQATESGADRPLLQNIEQFRKLHQNRRKS
                     YLEASLKMETGDRAVDAIAAEVAEVLGLKRIVVRTEQGDVE"
     gene            318..1040
                     /locus_tag="VEJ00_02325"
     CDS             318..1040
                     /locus_tag="VEJ00_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00023.1"
                     /translation="MGLAWTPAALAKRNDDKKDKAKSGGTQMVDSGSFGVFVKGQRVV
                     TETFSVEQRNGNSVVKSQLKQTTGTDSTSQKSDLEISSSGELLSYEWSQSSGGSLTVL
                     PNNEFLIERITAPGAKPAERPFLMPSSSTILDNNFFVQREVLAWKYLAADCKSEGGSL
                     KCQQGPVEFGALVPQDRTSMPVRMELVGKEKVTIRGSERELLRLNLKGENFEWALWVD
                     DHDQFKLMRVSIPADDTEVVRD"
     gene            1089..2174
                     /locus_tag="VEJ00_02330"
     CDS             1089..2174
                     /locus_tag="VEJ00_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXX00024.1"
                     /translation="MLLADARTARALVTVLLFALGLGFLYVARATLIAFLFAIFFAYL
                     MSPLVSNLEKLLKGRGRAIAVIYVLLLALIVLFFVVVGPKVTREGARLGKSLPDLLTR
                     LSSGQIAEQLGQEHGWNEKSTQLVQSFLASHGDDITQLAQRIGIRIADVAKQAWLLFV
                     VPLLSIFFLKDGRSFSDFLLSTIQSRPQREFLENVLNDLNQMLAHFIRAQLTLAALSF
                     VVYSAFLGLMRVPYALVLGTVGGLLEFVPVVGPLAAALIIVTVALLTSYPHWLVLVIF
                     LGIWRLIQDYVSSPRIMGESMELHPLAAIFGVMAGGEVAGILGIYLSIPVMASLRIVF
                     RRWRLYAEKKKFGPLTEYALGTQAHRK"
     gene            2366..3340
                     /locus_tag="VEJ00_02335"
     CDS             2366..3340
                     /locus_tag="VEJ00_02335"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXX00025.1"
                     /translation="MATLEVSFLHNQLEERKRRLEAAIAVAPRNASLAGLLREVDSAL
                     ERMDKGSYGICPECHEPVEQARLIADPLVRYCLDHLTQPQRDALQRDLDLASQVQRNL
                     LPQVGLRAGNWETSYHYAPVGPVSGDYCDLIPSDGQLFFVLGDVSGKGVAASMLMTQL
                     HALFRSLTGLALPLGQIVTQANRVFCESALAGQYATLVCGQAKPTGEVEIHNAGHWPA
                     IVVGRGGVLRIESTGLPMGMFHEGEFSATRLQLEVGDMLFLYTDGLSEARHADEEYGV
                     DRVTHLVRQHSARPPSELVAACLDDLRRFTNGGPAMDDLSLLAIRRSS"
     gene            complement(3373..4395)
                     /gene="galT"
                     /locus_tag="VEJ00_02340"
     CDS             complement(3373..4395)
                     /gene="galT"
                     /locus_tag="VEJ00_02340"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HXX00026.1"
                     /translation="MAELRQNFFTKEWVIIATERAKRPEELATHRTVASIPSFVETCP
                     FCPGNESKTPAEVMRFPANAGEPWAVRVIPNKFAALSSEVQPTRNLQHLRRRIDGFGF
                     HEVIIDTPDHSHCMALLPDSHVANILGVYRERYNALSVDRRVTHITIFKNHGADAGAS
                     LQHPHSQLIATPVIPSQVRHRLFEALRHYDDVGECMFCHMVEREVEDQTRIVLKGEHF
                     VAMQVFAAATPFATYIFPLRHMASFGDISDLEIADLARVLRTLLAKIYVGLENPDLNF
                     TVRSAPAEYAGARHFHWYVSVIPRLTRVAGFELGSGMFINTVLPEAAAEFLRKVSVEK
                     ALGAGA"
     gene            4537..>5051
                     /locus_tag="VEJ00_02345"
     CDS             4537..>5051
                     /locus_tag="VEJ00_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00027.1"
                     /translation="MIVMKFGGTSVEDAKAIDRVASIVEGRREQKPVVVVSAMAKVTD
                     TLLTMAKAAGAGERKTALKLCRSLQERHYNAASELLGTALFTEFHSELGAEFEALDEL
                     LRGISAVGEITPRTTDHVAAFGEMLSSKIVAAAFAARGLKGAHVDSRDVLITDSNYMQ
                     AVPQFEETNAKL"
BASE COUNT          960 a         1478 c         1605 g         1008 t
ORIGIN      
        1 gtttttctgg atgctccggt ggaggaactg tggcgccgct gttgcaagca ggctaccgaa
       61 tccggggccg atcggccgtt gctgcagaac atagagcagt tccgcaagct ccatcagaac
      121 cggcggaaga gctatctgga agcgtcactc aagatggaaa ccggcgaccg cgcggtagac
      181 gcgattgcag cggaggtcgc ggaggttctg ggtctgaaaa ggattgtggt acgtaccgaa
      241 caaggagatg tggagtgaag ctgcaagggg aacggcggcg gatcttgtcc gttgttgctg
      301 cggccctgat gcttatcatg gggctagcat ggacgcctgc ggctctggcc aagcgtaacg
      361 acgacaagaa ggacaaggcc aagtcggggg gcacgcaaat ggtcgactcc ggctcgttcg
      421 gggtcttcgt caagggccag cgggtcgtca ccgaaacctt cagcgttgaa cagcgcaacg
      481 gaaacagcgt ggtcaagtcg caactgaagc agacgaccgg cacggacagc acgagtcaga
      541 agtcggacct ggagatcagt tccagcggag aactgctgag ctatgagtgg agtcagtctt
      601 cgggcggctc tttgacggtt cttccgaaca acgaattcct gatcgagagg atcacggctc
      661 ccggcgccaa gcctgccgaa cgcccctttc tgatgcccag ctcgtcgacg attctcgaca
      721 ataatttctt cgtgcagcgc gaagtgctgg cctggaaata cctggccgcc gactgcaaat
      781 cggaaggcgg aagcttgaag tgccagcagg gtccggtgga gtttggcgcg ctcgtccccc
      841 aggaccgcac gtcgatgccg gtgcgcatgg aactggtggg caaagagaag gtgaccattc
      901 gtggctccga gcgcgaattg cttcgcctga acctgaaggg cgagaacttt gagtgggccc
      961 tgtgggtaga tgatcacgat cagttcaagt tgatgcgggt gtcgattcct gccgatgata
     1021 cggaagtagt ccgggactga gctttccctt cccggcggca gaatcgagaa catcccctgg
     1081 aacacctatt gctgctggcg gatgcacgta cggcgcgggc tctggtcacg gtgctgctgt
     1141 ttgcgctggg gctgggattt ctttatgtgg cgcgggctac cctgatcgcc ttcctgttcg
     1201 ccatcttctt cgcctacctg atgagcccgc tggtcagcaa cctggaaaag ctgctcaagg
     1261 gccgcggccg cgccatcgct gtgatctacg tactgctgct tgcactgatc gtgctgttct
     1321 tcgtggtcgt cggtccgaag gtgacgcgcg aaggagcgcg tctcgggaaa tcgctgccgg
     1381 acctgctgac acggctcagt tcgggccaga tcgcggagca gttggggcag gaacacggct
     1441 ggaatgaaaa gagtacgcag ctggtgcagt cgtttctggc cagccacggc gacgatatca
     1501 cgcaactggc ccagcgcatc ggaatacgca ttgccgatgt cgctaaacag gcctggcttc
     1561 tctttgtggt gcccctgctt tcgattttct tcctgaaaga cggccggtcc ttcagtgatt
     1621 tcctgctgtc gacgatccag tcccgcccgc agcgtgagtt tctggaaaac gtgctcaacg
     1681 acctgaacca gatgctggcg catttcattc gcgcgcagtt gaccctggcc gcgctctcgt
     1741 tcgtggtgta ttccgccttt cttggcttga tgcgcgtccc ctacgcgctg gttcttggca
     1801 cggttggcgg actgctcgag ttcgtcccgg ttgtgggccc gctggcggca gcattgatca
     1861 tcgtcacggt ggcgttgctg accagctatc cgcactggct ggtcctcgtg atttttcttg
     1921 gaatctggcg cctgatccag gactacgttt catccccgcg catcatgggc gagagcatgg
     1981 aactgcatcc tctggctgcc atttttggcg tgatggcggg cggtgaggtg gcgggcattc
     2041 tggggatcta cctttccatc ccggtcatgg ccagcctgcg catcgtcttt cggcgctggc
     2101 ggttatatgc cgagaagaag aaattcggtc cgctcaccga atacgctctg ggaactcagg
     2161 cgcataggaa ataggggcag cgcccgccag cttccagcgg cgccagtact ggcatggcgg
     2221 tctcaatccc cgcgagacgt tgctgcgcag gtgcctgacc caactggacg cgaggctatc
     2281 ctcggtaagt gccatcccag atccgcatcg aaaacacctc tggcagaatc taattggagg
     2341 atacttagga actaccgagg ttgctatggc cactttggaa gtgagtttcc tgcacaatca
     2401 actggaggag cgcaagcgtc gcctggaagc ggcgattgcg gttgcgccgc ggaacgcgag
     2461 cctggccggt ttgctgcgcg aggtggattc agcactggag cgcatggaca aaggcagcta
     2521 cgggatatgc ccggagtgcc acgagccagt ggaacaggct cgcctgatag ccgatccgct
     2581 ggtgcgttac tgcctggacc atctgactca gccgcaacgc gacgcgctgc agcgcgacct
     2641 cgatctggcc tcacaggtgc aacgcaactt gttgccacag gtgggtctgc gcgctggcaa
     2701 ttgggaaacc agctatcact acgcaccggt cgggccggtt agcggcgact actgcgactt
     2761 gatcccgtcc gatggccaac ttttctttgt gctgggcgat gtctcgggca agggcgtagc
     2821 cgcttccatg ctgatgacgc agctgcacgc tttgttccgc agcctgaccg gcctggccct
     2881 gccgctcggt cagattgtga ctcaagccaa ccgtgtcttc tgtgaaagtg cgctggccgg
     2941 acagtacgcc acgctggtct gcgggcaggc gaagcccacc ggggaggtgg aaattcacaa
     3001 tgccggccat tggcctgcga tcgtggtcgg gcggggcggc gtgttacgca ttgaatcgac
     3061 cggcttgccc atggggatgt tccacgaagg cgagttctcc gccacgcgcc tgcaactgga
     3121 agtcggcgac atgctgttcc tatacaccga cggcctgtcc gaagcccgcc atgccgatga
     3181 agagtatggc gtggatcggg tcacgcacct ggtgcgtcag cacagcgcac ggcccccgtc
     3241 cgagctggtt gcagcctgcc tcgatgactt gcggcggttt accaacggcg gtcctgccat
     3301 ggatgacctg tctctcctgg ccatccggcg cagtagctga gcagcgacgc gcggcggcga
     3361 ggaacagttg gactaagctc cagctccgag ggctttttcc accgagacct tacgcaggaa
     3421 ttccgccgcg gcttcgggca ggacggtgtt gatgaacatt cctgagccga gctcgaaccc
     3481 ggccacccgc gtcagccgcg gaatcacgct gacataccag tgaaagtgcc gtgcacccgc
     3541 atattcggcc ggggcgctgc ggacggtgaa gttgagatcc gggttctcca gcccgacgta
     3601 aatcttagcc agcagcgtgc gcaaaactct cgccaggtcg gcaatttcca ggtcgctgat
     3661 atctccaaag ctcgccatat ggcgtagggg aaagatgtag gtggcgaacg gcgtggctgc
     3721 ggcgaagacc tgcatggcga cgaagtgttc gcccttcagc acgatgcgcg tctggtcttc
     3781 gacctcgcgc tccaccatgt ggcagaacat gcactcgcct acgtcgtcgt agtggcggag
     3841 agcctcgaac aggcgatgcc gaacctggct cgggatcacc ggcgtggcaa tgagctggga
     3901 gtgcggatgc tgcaggctcg cgcccgcatc ggctccgtgg tttttgaaga tcgtaatgtg
     3961 ggtcacgcga cggtccacgc tcaaggcgtt gtagcgttcc cggtagacgc ccaaaatgtt
     4021 ggcgacatgc gaatcgggca gcagtgccat gcagtgggag tggtccggag tgtcgatgat
     4081 gacttcgtga aagccgaagc catcgatgcg gcgccgcagg tgctggagat tgcgcgtggg
     4141 ctggacttcg ctggacagcg cggcaaactt gttgggaatc acccgcaccg cccagggctc
     4201 gcccgcgttg gccgggaacc gcatcacctc ggccggcgtc ttgctctcat tgccgggaca
     4261 gaacgggcag gtttccacaa acgacggaat cgacgccaca gtacgatggg tcgcgagctc
     4321 ttccgggcgc ttcgcccgct ccgtggcgat gatgacccac tctttggtaa agaagttctg
     4381 acgcagctcg gccatgagag ttcctccgaa cgtttctatc ggcagaagcg ggaaaaatct
     4441 gaagtcacgc tgttatagaa ttcaaactat cgttatgagc cgcccaactg cgctcgaccc
     4501 gcgatttccg gccgcctccg caaggaagcc tcgcctatga tcgtcatgaa gtttggcggc
     4561 acctcggtag aagatgccaa ggcgatcgac cgcgtagcct cgatcgttga gggccggcgg
     4621 gagcagaaac ctgtggtcgt ggtcagtgcc atggccaagg tgaccgacac gctgctcact
     4681 atggccaagg ccgcgggcgc aggcgaacgc aagaccgcgc tcaaactttg ccgctccctg
     4741 caggaacgcc actacaacgc agccagcgaa ctgctcggca ccgcgctgtt cacagaattc
     4801 cattccgagc tgggcgcaga gtttgaggcg ctcgatgagc tactacgcgg aatttccgct
     4861 gtcggcgaga tcacgccccg cactaccgac catgtcgccg ccttcggcga gatgctctcg
     4921 agcaagatcg tggcggccgc atttgccgcc cggggattga agggcgcgca cgtcgattcc
     4981 cgcgacgtgc tgatcaccga cagcaactac atgcaggcgg ttccgcagtt cgaagagacc
     5041 aacgccaagc t
//
LOCUS       DASPUL010000101         7454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_17761, whole genome shotgun sequence.
ACCESSION   DASPUL010000101 DASPUL010000000
VERSION     DASPUL010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7454)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17761"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VEJ00_02350"
     CDS             complement(<1..256)
                     /locus_tag="VEJ00_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00028.1"
                     /translation="MAKEMTFGHPMFAESMLETSWSQRSRRGWTTLTSFGVQAIVIGG
                     VILMALLRSVVIPDARVVSTPVTMGRPEPEPIAQRPRGGSS"
     gene            complement(394..1116)
                     /locus_tag="VEJ00_02355"
     CDS             complement(394..1116)
                     /locus_tag="VEJ00_02355"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HXX00029.1"
                     /translation="MTSKFLRSFLMIAALCAISAFAQTSSAATPPNAPSAAAAQPSTT
                     SATGTKVGTININEAIFGTNEGQRDMDGLGKKFEPKQAELKNQNDELEGLKKQLTNTQ
                     DKLNPDALDTLKKQIETKQKTFDRAVQDAQEDFGNQRQEIAGKILKKLAPMIVKYSQD
                     NGFSLIVDTSNPWPQSPVLWWGESVDITKPVVDEYNKQSGVPAPAAAGAAVPKPAAPK
                     PATGTGTAPKPPAPKPTEPPKN"
     gene            complement(1400..4216)
                     /gene="bamA"
                     /locus_tag="VEJ00_02360"
     CDS             complement(1400..4216)
                     /gene="bamA"
                     /locus_tag="VEJ00_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXX00030.1"
                     /translation="MRAAHVLVAIGLTLTMLVAMAGSLWAQGELITGITVTGNRRIPQ
                     ETIKARIFSKAGDVYDPSALERDFNSLWNTGYFEDIKFLREQTPKGWDIIVQVKEKPT
                     IREINYVGLSSVSTSDVLDRFKQAKVGLSVESQYDPTRIKKAEVTIKELLSEHGRQFA
                     TIRSEVRQIPPAAVGITFVVKEGPKVKVGKIKFDGNKNIKSRLLRAAMKNLRPIGVPH
                     SIFLENIFAKTYDATKLEEDTERVRAEYQNRGYFKVVVNDPKTQIHDTGHKGFHIPLL
                     QPGAGKAVDLTMPIEEGDRYRLGKITFKNNKAITNTTALRALFPIKDGDIFSREKVAK
                     GLENLRTAYGQYGYINYTGVPSTTFDDEKKLAFLEIDVDEGKQFYVRRIEFAGNTTTR
                     DKVIRRELALEEGGIYNSKLWELSLLRLNQLSYFDQLKADDPNVTDKKLDEKNGYVDL
                     TLHVREKGKNSIGLNGGVSGLEGAFIGLNYSTNNFLGRGETLQVQLSLGNLARSALFG
                     YTQPYMFDRPLQFGFTVFGNKVSFNQARQLSIFSGQNLNLPSAVLQNLQNYSQSSVGF
                     STSLSYPLRRSFKRVGITYSLDRSTLLPLSTASKSLFDFIAFRGISGPNAVNGIITSK
                     IFPNFSFNTLDSGISPHHGHQFTAGAEFAGLGGTVRSIRPIVQYKRFTPMQKGRNAFG
                     FNVDGSFISGFAGLVAPPFQRFYMGGENDIRGFDIRSVSPVAFLPSANVITLTTPDRK
                     PVLRDPLNPSLGNYTIPIPVDQIVFPGGDLSVWTNFEYRITIAGPVAIAPFWDTGIDP
                     IIRRSQLQIANVQYDSVISTIFGCPQLDSSFNCIGGARLNPPPSQQLQVLGTTNWKPR
                     ASTGIELQMFLPVVNAPFRIYWAYNPLRLDEMTTPPIAITRAMFPPGGAGDFTYNLAK
                     QTFAPQFLLREPRKTFRFTVATTF"
     gene            complement(4526..6715)
                     /locus_tag="VEJ00_02365"
     CDS             complement(4526..6715)
                     /locus_tag="VEJ00_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXX00031.1"
                     /translation="MKRVVHAACPHDCPDACGVLITIEDGRATKIQGDPEHPVTRGFL
                     CAKVAKYLDRVYSPDRVLYPARRIAPKGPVTDQDPHSSRKERARNGAPDVASGVWQRI
                     SWDEALDEITTRFRATSSEFGSEAILPYSYGGTIATLNGSTMDHRFFHRLGASQLERT
                     ICSAAGEAGLKSVLGVKLGTEPEQFRHSKYIIAWASNIHGNNVHLWPFITEARRNGAK
                     LVVIDPYRTRTAECSDWYLPINPGSDAALALAMMNVIIGENLHDAGYVAKYTLGFDQL
                     REKVKEYAPERVAQWTGISAADIRKLTHEYATTRPSVIRLNYGVQRSERGGMATRAIA
                     MLPCILGSWKEVGGGLQMSLSGAFAFDDAALKRPDLMQTALGREARTINMVELGRVLN
                     TQNDPPVKALFVYNSNPAAVCPQHNDVVRGLMRPDLFTVVHEQFFTDTTDYADIVLPA
                     TTFFEHKDIQKSYGHYYLQVSDQAIDPLGECRSNVEVFRALALRMGFEEQCFRETVDE
                     MIDTALDSRDPWLKGISRERLERDHAVRLNLASSGSRHPASGDSPHAEPFLPFAQGNF
                     RTPSGKALLYNEELIAQGLDPVAEFTPPSESRHGVLQKAVPLELLARKADNFLNSTFS
                     NLPSVQEMEEPGLLEICAVDASARGIADGDAVRVFNRRGEILLKARVDGKVQPGVVSA
                     TLNWAKMTAGFQSINALTSEKLTDMGNSATFYSVLVEVELAKPKISG"
     gene            complement(6873..7448)
                     /gene="frr"
                     /locus_tag="VEJ00_02370"
     CDS             complement(6873..7448)
                     /gene="frr"
                     /locus_tag="VEJ00_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXX00032.1"
                     /translation="MASIAGLKDTYAQLKTRMEKAVEDFRKAMAATRTGRANVHMLDG
                     VTVDAYGSQMPLNQVAQVHAPEAQLITVQPFDPSQLGAIEKAIRGGDLGLNPMNDGKI
                     IRVPVPPLTQERRQEMVKHLHKALEDHRTAVRNIRRDGNDAIKRALKDKKITEDEEKR
                     SLDEIQKLTDDEIKKMEEMSKAKEKEVLELK"
BASE COUNT         1409 a         2142 c         2268 g         1635 t
ORIGIN      
        1 ctgaagatcc gccacgcggg cgctgggcaa taggctcggg ctcaggtcgt cccatcgtca
       61 ccggcgtgga aacaacccgt gcgtcgggga ttacgaccga ccttagcagg gccatcagaa
      121 tcaccccgcc gatgacgatc gcttgcactc cgaacgaggt caaagtggtc cagccccggc
      181 gcgagcgctg ggaccaggaa gtttccagca tgctttcggc aaacattggg tggccaaatg
      241 tcatttcctt agccacggcg tcctccagcg tggaatacct ttgctgctat ggacaccggg
      301 agggatcgct acggcggcgt tgctgcaaat aaaaaaggcc gcatttttac gcggcctgcg
      361 ggaacttttt cagcgcaaaa ggtgtcgagc tcctcagttc ttgggcggct cggtgggctt
      421 gggagcgggc ggcttcggag ctgttccggt tccggtagca ggcttgggag cagcaggctt
      481 gggaacagct gcgccggccg cagctggcgc aggcacaccc gactgcttgt tgtattcgtc
      541 cacaactggc ttggtgatgt cgaccgactc gccccaccac agcaccgggc tctgcggcca
      601 cgggttggat gtgtccacaa tcaggctgaa gccgttatcc tgcgaatact tcacgatcat
      661 gggcgccagc ttcttcagaa tcttgccggc aatttcctgg cgctgattgc cgaagtcctc
      721 ctgcgcgtcc tgcacggcgc ggtcgaaggt tttctgcttg gtctcgatct gcttcttcag
      781 cgtgtcaaga gcatcgggat tcagcttgtc ctgcgtattc gtcagctgtt tcttcaggcc
      841 ttccagctcg tcattctggt ttttcagctc ggcctgtttg ggctcgaatt tcttgccgag
      901 cccgtccata tcgcgctgac cttcgttcgt gccgaagatg gcttcgttga tgttgatcgt
      961 cccgaccttg gtgccagttg ccgaagtcgt cgaaggctgg gcagccgctg cggacggagc
     1021 attcggcggc gtcgcagcac tgctggtttg cgcgaaggcg gaaatcgcgc ataaagccgc
     1081 aatcatcagg aaagaacgca agaacttgct tgtcattgga tcgattgaac tccttggaac
     1141 acgtttcttc gcactatagt tccgggaatg aactctgatt tcgttctcaa gatcgcttat
     1201 cactcagcgc cgggccgcac tgaacgaggt ctccgctgcg gtagcggccg ccgtcggaat
     1261 tggcgcactg gattgccggc gtcacacgcg cgaaactaca ggcgtgccat ccaccactca
     1321 agctctgtcg attggtgtgg atcgcaagca ttatagagac ctcgcgcaca cgccgtcaaa
     1381 cctgttcact catgccaact tagaacgtgg tggccaccgt gaagcggaac gtcttccgcg
     1441 gctcgcgcag caggaactga ggagcaaagg tctgtttggc cagattgtag gtgaagtctc
     1501 ccgctcctcc cggggggaac atggcgcggg taattgcgat cggcggagtg gtcatctcat
     1561 ccagccgcag cgggttgtaa gcccagtaga tgcggaacgg ggcgttgacc acgggcagga
     1621 acatctgcaa ctcgatcccg gtcgaggcgc gaggtttcca gttcgtcgtt cccagcacct
     1681 gcagctgttg cgacggcgga gggttcaggc gagctccgcc aatgcagttg aaggacgaat
     1741 ccagctgtgg gcaaccgaaa atggtgctga tcacgctgtc atattgcacg ttggcgatct
     1801 gcaactgcga gcgccggatg atggggtcga tgccggtgtc ccagaacggc gcgatcgcca
     1861 ccgggccggc aatggtgatc cggtattcga agttcgtcca gacgctgaga tcgccgccag
     1921 ggaacacgat ctgatcgacc ggaatgggga tggtgtagtt gcccaggctt ggattcaacg
     1981 ggtcgcgcag gaccggcttg cggtcgggag tggtcagagt gatgacattg gcgctgggca
     2041 ggaacgccac cggtgacaca gagcggatat caaacccacg gatgtcgttt tcgccgccca
     2101 tatagaagcg ctggaagggc ggggccacga gccccgcgaa gccgctgatg aacgatccat
     2161 cgacattgaa cccgaatgca ttgcggcctt tctgcatcgg agtgaaccgc ttgtactgca
     2221 cgatcgggcg gatcgagcgg accgtgccgc ccagcccggc gaattcggct ccggcggtga
     2281 attggtggcc gtgatgcgga gaaataccgc tgtccagcgt gttgaacgag aaattgggga
     2341 agatcttgct ggtgataatt ccattcacgg cgttggggcc ggaaatgccg cggaaggcga
     2401 tgaagtcgaa cagactcttg gacgcggtgc tgagcgggag cagggtcgag cggtccagcg
     2461 agtaggtgat gccaacgcgc ttaaacgaac ggcgcaacgg atagctcaac gaggtggaga
     2521 atccgacgct cgactgcgag taattttgca ggttctgcag cacagcgctg ggcaggttca
     2581 gattctgtcc gctgaagatg gaaagctgcc gggcttggtt gaagctgacc ttgttcccga
     2641 acaccgtgaa gccgaattgc agcggccggt caaacatgta gggctgggtg tatccaaaca
     2701 gggcgctgcg cgccaggttt cccaggctga gctgtacctg cagggtctcg ccgcggccga
     2761 gaaagttgtt ggtggagtaa ttcagcccga tgaacgctcc ttccagcccg ctgacgccgc
     2821 cgttgagtcc gatgctgttc ttaccctttt ccctgacgtg gagcgtcagg tcgacatagc
     2881 cgttcttctc gtcgagcttc ttatctgtca cgttgggatc atcggcctta agctggtcga
     2941 aataggaaag ctgattcagg cgcagcaggc tgagttccca caatttcgag ttgtagatgc
     3001 cgccttcttc cagcgccagt tcgcggcgga tgaccttgtc gcgtgtagtt gtgttgccgg
     3061 caaactcgat ccggcgaacg tagaattgtt tgccttcgtc cacgtcgatc tcgagaaacg
     3121 cgagtttctt ttcgtcgtcg aaggtggtgc tcggaactcc cgtgtagttg atgtatccgt
     3181 attgaccgta agcggtgcgc aggttctcca ggcctttggc gaccttttcg cggctgaaga
     3241 tatcgccgtc tttgatgggg aacagcgcgc gcaaagctgt cgtgttggtg atggccttgt
     3301 tgttcttgaa ggtaatcttg cccagtcggt agcgatcgcc ttcttcgatc ggcatagtga
     3361 gatcgacggc ttttccagcg cccggctgca gcagggggat gtggaagccc ttgtgcccgg
     3421 tgtcgtgaat ctgggtcttg ggatcgttaa ccacaacttt gaaatagccg cggttctggt
     3481 actcggctcg cacccgctcc gtgtcttctt caagcttggt ggcgtcgtag gtcttggcga
     3541 agatgttctc caggaagatc gagtgcggaa caccgatggg ccgcaggttc ttcatggccg
     3601 ctcgcaggag gcgcgacttg atgttcttgt tcccgtcgaa cttaatcttg ccgactttga
     3661 cttttggccc ttccttgacc acaaacgtga tgccgaccgc ggcgggcggg atctggcgga
     3721 cttccgagcg gatggtggcg aactggcgac cgtgttccga gagcaattcc ttgatcgtaa
     3781 cctcggcctt cttgatgcgg gtgggatcat attgactttc aacactcagc ccgaccttcg
     3841 cctgtttgaa gcggtcgagc acatcgctgg tcgagacgga actaagacca acgtagttga
     3901 tctcgcggat ggtcggcttc tctttcacct gaacgatgat gtcccaccct ttcggggttt
     3961 gctcgcgcag gaacttgatg tcttcgaaat aaccggtgtt ccacagcgaa ttgaaatcgc
     4021 gctccagagc cgagggatcg tagacatctc cagccttcga aaaaatgcgc gccttgatgg
     4081 tttcctgcgg gatgcggcgg ttgccggtga ccgtaatgcc ggtgatgagc tcgccttgcg
     4141 cccacaggct gcccgccatc gccacgagca tggtcagcgt gagcccgatc gcaaccagga
     4201 cgtgcgccgc tcgcatgtcc aaaccggatc gcctcagttt agtccaagcc cgttggaatg
     4261 aaggagatgg cacggacatg cggctcccgg tacacgcatg atcgggcgca acaaaactct
     4321 ccggatgaaa gcgaaaagga atcgctacga cctcagcctc tgggatgtaa gaaaactgtc
     4381 attatacggt agagcaacgg aaagcgtcca agccgcaact ctcggtgttt agtcctttgg
     4441 ttgctttgac tgaccaggat tctgacggat gacgggcccc gatcaccgag caccagatcg
     4501 ttgcccccgg ccaccccgcg cgacactagc cactgatctt tggcttggct aactctacct
     4561 caaccaagac ggaataaaac gtggccgagt tgcccatgtc ggtaagtttt tctgaggtca
     4621 gggcgttgat gctctggaac cccgcggtca ttttggccca attaagtgtg gcagagacga
     4681 ccccaggctg aactttgcca tccacccgcg ccttgagcag gatttcccca cgccggttga
     4741 aaacgcggac agcgtcgccg tcggcgatgc cgcgagcgct tgcatcgaca gcacaaatct
     4801 caagcagacc gggctcttcc atttcttgca ccgatggcag gttggagaat gtggaattga
     4861 ggaaattgtc ggccttgcga gcgagcagtt ccagcggaac cgctttctgc agaactccat
     4921 gacgggattc ggacggcggg gtgaactccg ccaccgggtc gagcccctgc gctattaatt
     4981 cctcgttgta aagcagagcc ttgcccgaag gcgtgcggaa gttgccctga gcaaagggaa
     5041 gaaacggctc ggcgtgagga gagtctccgg aagccggatg ccgggagccg gacgacgcca
     5101 agttcaagcg tactgcatga tcgcgctcca gacgctcgcg actgatcccc ttcagccagg
     5161 gatcgcgcga atcgagcgcc gtgtcgatca tctcgtctac cgtctcgcgg aagcattgct
     5221 cctcgaaccc catgcgcagc gccagcgctc ggaagacttc cacgttcgag cggcactcgc
     5281 ccagcggatc aatcgcctga tccgaaactt gcagatagta gtgcccgtaa gatttttgta
     5341 tatctttgtg ctcgaagaac gtcgttgccg gcagaacgat gtcggcataa tcggtcgtat
     5401 ccgtgaaaaa ctgctcgtgc acgacggtga acaaatccgg ccgcatgagg ccgcgaacca
     5461 cgtcgttgtg ctgcggacag accgccgcgg gatttgagtt gtagacgaac aaggctttca
     5521 cgggcgggtc attctgcgtg ttcagcacgc ggcccagttc caccatgttg atggtacggg
     5581 cttcgcgtcc gagagccgtc tgcatcaaat cgggacgctt gagagcagcg tcgtcgaagg
     5641 caaacgcgcc gctcaaggac atttgcagtc cgccgccgac ttccttccac gatcccagga
     5701 tgcagggcag catggcgatc gcgcgagtag ccatgccgcc gcgctcggag cgctgcacgc
     5761 cgtaattgag ccggatcacc gatggccgag tcgtcgcgta ttcgtgggtg agtttgcgaa
     5821 tgtcggccgc gctgatgccg gtccattgcg ccacgcgctc tggagcgtat tccttcactt
     5881 tttcgcgcag ttggtcgaag ccgagcgtgt acttcgcgac gtagccggca tcgtgcaggt
     5941 tctcgccgat aatgacgttc atcatggcaa gcgccagcgc cgcgtcgctg ccgggattga
     6001 tcggcaaata ccagtcggag cattcggccg tgcgcgtgcg ataaggatcg atcacgacca
     6061 gcttcgcgcc gttgcgtcgc gcctcggtga tgaaaggcca caggtgcacg ttgttgccgt
     6121 gaatgttcga ggcccacgcg atgatgtact tggaatggcg gaactgctcg ggctccgttc
     6181 ccagcttcac gcccagcacc gacttcagcc ccgcttcacc ggccgcggaa caaattgtcc
     6241 gctcgagctg cgaggctccc aggcgatgga agaacctgtg atccatcgtc gaaccgttca
     6301 acgtggcgat cgtgcctccg taggaataag gcagaatggc ctcgctgccg aactcggacg
     6361 aagtggcgcg aaagcgcgtc gtgatctcgt caagagcttc gtcccaggaa atacgttgcc
     6421 agactccaga agcgacgtcg ggtgccccat ttctcgcgcg ctctttgcgc gaggagtggg
     6481 gatcttgatc tgtcaccggc cccttcggtg caatccgtcg tgcgggatag aggacccgat
     6541 ccggcgagta cacccggtcc agatacttcg cgaccttggc acacagaaac ccgcgcgtga
     6601 cggggtgttc ggggtcgccc tggatctttg tcgcgcgtcc gtcttcaatg gtgatcagca
     6661 caccacaggc atcgggacag tcatgcgggc acgcggcatg gacgacgcgc ttcatacagc
     6721 ttcaattata ggggagtacg ggctatgtag ggacagtcgc cctcggttgt cctcgggccg
     6781 aaggcccgat cttgtttttc atctcctggc gacaggagat cgccgtcgag cttcgctcga
     6841 ccggacagcc ggggcggctg tccctacatg aactacttca actccagaac ttctttttct
     6901 ttcgctttgc tcatctcttc catcttcttg atttcgtcgt cggtgagctt ctggatttcg
     6961 tcgagcgagc gcttctcttc gtcttcggtg attttcttgt ctttcagcgc cctcttgatg
     7021 gcgtcgttgc cgtcgcggcg gatgttgcga acggcagtgc ggtggtcttc gagtgccttg
     7081 tgcaggtgct tgaccatctc ctggcgacgc tcctgcgtca aaggcggaac aggtacgcgg
     7141 atgatcttgc cgtcattcat gggattcagg ccgaggtcgc cgccgcggat ggccttttcg
     7201 atcgcgccca actgcgatgg gtcgaacggc tgcaccgtga tcagctgcgc ctccggcgcg
     7261 tgcacttgcg ccacctgatt cagcggcatc tgcgagccgt aggcatccac ggtcacgccg
     7321 tcgagcatgt gcacgtttgc ccgccccgtc ctcgtagctg ccatcgcctt gcggaaatct
     7381 tccaccgctt tctccatgcg cgtcttcaac tgcgcatagg tgtctttcag cccggctatc
     7441 gatgccatgg tcgc
//
LOCUS       DASPUL010000102         4098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_837924, whole genome shotgun sequence.
ACCESSION   DASPUL010000102 DASPUL010000000
VERSION     DASPUL010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837924"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1734
                     /locus_tag="VEJ00_02375"
     CDS             <1..1734
                     /locus_tag="VEJ00_02375"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HXX00033.1"
                     /translation="ELIRGIHREYLQAGAEIIETNTFGANSFRLARHSMGDKVRDINR
                     AGARLAREAAKSFDVWVAGSVGPLGARIEPLGKTSFQEARDAFREQISTLVEGGVDLL
                     ILETFGYLEEIHQAMLAAREVNASIPLVAQVTIDEDGNCLDGSDPETFAPKLEEWGAD
                     AIGCNCSVGPVAMLDAIERVRAISSLPLAAQPNAGIPRSVEGRNIYLCSPEYMASYAR
                     KFVAAGVRIVGGCCGTTPDHIRTMKSALRVGEARSKTTSGPMAGSHATPAAVPAIPLD
                     GRSRLGAKLARGEFVTMVEIVPPKGTDIRKEVEGARFLKSVGVDAVNIPDSPRASARM
                     SNQALSLLIQREVGIDAILHYTCRDRNVLCIQSDLLGAAAVGIRNLICITGDPPKMGN
                     YPDATAVFDVDSIGLVNIVRNLNHGLDIGGNPIGTGTGFVIGVGANPGLTDLDEEIRR
                     FEYKVEAGAEYAVTQPVFDLRLLENFLRRIEHCRIPLIAGIWPLVSVRNAEFMKNELR
                     VSVPDSILERMARAQTPEAAREEGVAIAREMLMASRHIVQGAQISAPLSRYSSAVDVL
                     EALGSVRPASA"
     gene            1859..2098
                     /gene="xseB"
                     /locus_tag="VEJ00_02380"
     CDS             1859..2098
                     /gene="xseB"
                     /locus_tag="VEJ00_02380"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXX00034.1"
                     /translation="MAKFEECLQRLERIVQELEKGDVPLEKSLTLFEEGMQLSSACRK
                     ELEQAEGKVEILLKKNGKLQPEAFEPLTDKTSMRR"
     gene            2212..2577
                     /locus_tag="VEJ00_02385"
     CDS             2212..2577
                     /locus_tag="VEJ00_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00035.1"
                     /translation="MAQSVVVLEKDPGVARSLAGGLRPHFSVHIVQSRDELRERFAED
                     HPEALVLNIEHWRLNEVTNLHRDFPALPIVCTHRVPDEEMWMAALEAGASDVCAADDV
                     DNVLTSVLRSMALSRTAAA"
     gene            2734..3615
                     /locus_tag="VEJ00_02390"
     CDS             2734..3615
                     /locus_tag="VEJ00_02390"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HXX00036.1"
                     /translation="MLQETLEQGQRLTDAALDRLIPLETQHPVSIHKAMRHSVFAGGK
                     RLRPVLAMEAGRMVAGSLPAGIEELGAALEMLHTYSLIHDDLPALDNDDLRRGRPTCH
                     KAFGEALAILAGDALQTQAYEVLSRLKCPAEARVRIIEEIARGTGTVDGMIGGQVVDL
                     EAEHTKPTPEMLEYIHRSKTAALITSSLVSGGVYAGAKDSQVATLRAFGMRIGLAFQI
                     VDDVLDVTQTSEQLGKTAGKDTTSEKATYPALFGIEASLRKADALVSDAFNELDGFGE
                     RAATLKGLARFLVERKK"
     regulatory      3675..3740
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            3767..>4098
                     /gene="crcB"
                     /locus_tag="VEJ00_02395"
     CDS             3767..>4098
                     /gene="crcB"
                     /locus_tag="VEJ00_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HXX00037.1"
                     /translation="MKDFLAISVAAIVGANLRYLLSRFAARELGPVFPYGTLGINIVG
                     SFIVGFFVVWTTERALVDPRWRLLVVVGFCGSFTTFSSYAYESMSYFETGQWGLMLAN
                     IFSNNLLCL"
BASE COUNT          816 a         1203 c         1279 g          800 t
ORIGIN      
        1 gaactgatcc gcggcattca ccgcgagtat ctgcaggcgg gcgcagaaat cattgaaacc
       61 aatactttcg gcgccaattc cttccgcctc gcccgccata gtatgggcga caaggtgcgc
      121 gacatcaacc gtgccggcgc acgcctggct cgcgaagcgg ccaaaagctt cgatgtgtgg
      181 gtggcgggat cggttggccc gctgggcgcc agaattgagc ctctgggcaa gacatcgttc
      241 caggaagctc gcgatgcctt ccgcgagcag atctctacac ttgttgaagg cggagtcgac
      301 ctgctgattc tcgagacctt cggctatctg gaagaaatcc accaggccat gttggctgcc
      361 cgggaggtga atgcgagcat tcccctcgtt gcgcaggtga cgatcgatga agatggcaac
      421 tgcctggacg gctccgatcc ggaaacgttt gctcccaagc tggaggaatg gggagccgac
      481 gcgattggct gcaactgcag cgtcggtccc gtggccatgc tcgatgcgat cgagcgcgtg
      541 agggcgattt cgtctctgcc cctggccgca caacccaacg caggcattcc ccggtcagta
      601 gaaggcagaa acatttacct gtgctcgccg gagtatatgg cgagctacgc ccgcaagttc
      661 gtggccgctg gagttcgcat cgtaggcgga tgctgcggaa caacgcccga ccacatccgc
      721 accatgaagt ccgctctgcg agtcggcgag gctcgaagca aaacgacttc cgggccgatg
      781 gcaggcagtc acgccacgcc agccgcagtt ccagccatcc cgctggatgg tcgctcccgt
      841 ttaggggcca aactggcgcg cggcgaattc gtgaccatgg tcgagatcgt gccgccgaag
      901 ggaaccgaca tccgtaagga ggtcgaaggg gcgcgattcc tgaagtcggt gggggtggat
      961 gcagtcaaca tcccggacag cccgcgggcc tcggcgcgca tgagcaatca ggcgctctcc
     1021 ctgctcattc aacgtgaggt aggaatcgac gcgatcctgc actatacctg ccgcgaccgg
     1081 aatgtgctct gcatccagag tgatctgctg ggtgcggcgg ccgtgggaat caggaacctg
     1141 atctgcatca cgggcgatcc tcccaagatg gggaactatc ccgacgcgac ggcggtattc
     1201 gacgtggatt cgataggtct ggtcaacatc gtccgcaacc tgaaccatgg gctcgacatc
     1261 ggcggaaacc ccatcggcac cggcactggc tttgtcatcg gcgtaggggc gaacccgggc
     1321 ctgaccgatc tggatgagga gatccggcgc tttgaataca aggtcgaggc cggggcagaa
     1381 tatgcggtca cgcagcctgt gttcgacctg cgtctgctcg agaacttcct gcggcggatc
     1441 gagcactgca ggattccgct catcgcgggg atttggccac tggtgagcgt gcgcaacgcc
     1501 gaattcatga aaaatgagct gcgggtgtcc gtaccggact cgatcctgga gcgtatggcg
     1561 cgggcgcaga cgccggaagc tgcccgggag gaaggcgtgg ccattgcacg ggagatgctg
     1621 atggcctcac gccacatcgt tcaaggagcc cagatcagcg ctccactgag ccgatacagt
     1681 tcggccgtgg acgtgttgga agcattgggc agcgtccgtc ctgcctcggc gtgagtcgga
     1741 tccccaggtg tcccgattcg ggagtcgtcc ggtcggccac tgtggatatt gtcctaaaaa
     1801 gattggacac ttcgccggta tagttggatt aaaataacca gacaggagcg acgccccgtt
     1861 ggccaaattt gaagagtgcc tccagcgcct ggagagaatc gtccaggaac tggaaaaggg
     1921 agatgtcccg ctagagaaat ctctcacact gttcgaagag ggcatgcagc tttcctcggc
     1981 ctgccggaaa gaactggaac aggccgaagg caaggtggaa attctgctga aaaagaacgg
     2041 gaagctccag ccagaagcgt ttgagccgct gacggataag acgtctatgc gccggtaggt
     2101 gttaggccct cagcccggct cgcaatttga gatgtctgtg gagttggggt ctcccagccc
     2161 gccccgcagc ctgagtccgc cgttgacgac ggttgagaga tcgggggatg tatggctcaa
     2221 agtgtagtgg ttctggagaa agatcctggg gtggcccgct cactggccgg tggactgcgc
     2281 cctcactttt ccgttcatat tgtccagtcg cgcgatgaat tgcgggagag gtttgccgag
     2341 gatcatcccg aggctctggt actcaacata gagcactggc ggctcaacga ggtaacgaat
     2401 ctgcaccgcg attttcccgc gttgcctatc gtgtgcacac accgcgttcc ggatgaggag
     2461 atgtggatgg ccgcgctcga agccggcgcc agcgacgttt gcgctgccga cgatgtggat
     2521 aacgtcctga cctcggttct ccgaagcatg gccctgtcgc ggaccgccgc agcttaaagc
     2581 tagccgattc tccagaggac gcctgcgggc gtccttcttc ttttcctggc tcgctcggtc
     2641 atggctctga ccatcgagcc aagacccacg acgatcgcgg gcagactcgg gcgccacgcc
     2701 ccgccgctgc caccttgcta tactctctgc ttcatgttgc aggaaactct cgaacagggg
     2761 cagcggctga ccgatgccgc gctcgaccgc ctgattccgc tcgagacgca gcatccggtt
     2821 tcgattcaca aggcgatgcg ccatagcgtg ttcgcgggcg gcaagcgcct gcgccccgtg
     2881 ctggccatgg aggcgggacg catggtcgcc ggatcgttgc cggcaggcat tgaggaactc
     2941 ggcgccgcac tggaaatgct gcacacctac tcgctgatcc acgacgatct tccggcgctc
     3001 gacaatgacg atcttcgccg cgggcggcca acctgtcaca aggcttttgg tgaagccctc
     3061 gcgatcctgg ctggcgacgc gctgcagacc caggcgtatg aggtcctgtc gcggttgaaa
     3121 tgcccagcgg aggcaagggt acggatcatc gaagaaattg cccgcggcac cggcaccgtc
     3181 gacggcatga tcggcggaca agtcgtcgat ctcgaagccg aacacaccaa acccacgccc
     3241 gagatgctcg aatacattca tcgctcgaag accgccgccc tcatcacgtc gagcctggtg
     3301 agcggagggg tgtacgccgg agcgaaggat tcacaggtag cgacgctgcg cgctttcggc
     3361 atgagaatcg gtctcgcgtt ccagattgtg gatgacgtgc tcgacgtcac ccaaacttcc
     3421 gagcaattgg ggaaaacagc gggtaaagac acaacctcgg agaaggcgac gtaccccgcc
     3481 ctattcggga ttgaggcttc gctccggaag gcggatgctt tggtaagcga cgcattcaac
     3541 gaactggatg gtttcggcga acgggctgca acgctgaaag gactggcgag gttcctggtg
     3601 gaacggaaga agtaaccctg gcgccagact ggagcttttt aacctctgtg ctccactgtg
     3661 tcctgtgtgg taaaaaataa gacggcgatg gagttcgccc ataaccgcct gctgcaccgg
     3721 ctgatgactc ctacagacga aaattcaggt ttgtaggagc gctcgcgtga aagacttcct
     3781 cgccatctcc gtggctgcca tcgttggcgc caacctgcgt tacctgctca gccgcttcgc
     3841 tgcccgtgag ctgggtcccg tcttccccta cggaacgctc ggtatcaaca tcgtcggtag
     3901 cttcatcgtc ggattttttg tcgtctggac caccgaacgc gcgctggtcg acccgcgctg
     3961 gcgactgctg gtcgtcgtgg gcttttgcgg ttcgtttacc accttttcga gttacgccta
     4021 cgagagcatg tcgtactttg agactggcca atgggggctg atgctggcga acattttcag
     4081 caacaacctt ctctgtct
//
LOCUS       DASPUL010000103         5910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_427391, whole genome shotgun sequence.
ACCESSION   DASPUL010000103 DASPUL010000000
VERSION     DASPUL010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427391"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VEJ00_02400"
     CDS             complement(<1..894)
                     /locus_tag="VEJ00_02400"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXX00038.1"
                     /translation="MLKPGDIAIVSGARTPFGRYCGKLKDFTAQELGAIAAKGAIERS
                     GIDAKEFDHVVMGNAQQTSGDALYGARHVGLRAGIPIETPALTVNRLCGSGMQAIVSA
                     AQMIQTDEAKIVLAGGMEAMSQAPFVIRGRDAFTLAPGGKLEDSLMVALLDSYCGSYM
                     ANTAELYASEQGITRQMQDEFALRSQQNADAGYTEGRIREELVPVPLRNSKGEPTGEQ
                     LTEDDHRRPQTTMEGLAKLKPAFGKNGTVTAGNASGIVDGAAAVVVMAIEEARKRQIE
                     PLGRIVAWGIAGVEPKLMGRGP"
     gene            complement(1044..3506)
                     /locus_tag="VEJ00_02405"
     CDS             complement(1044..3506)
                     /locus_tag="VEJ00_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00039.1"
                     /translation="MRWLRSGSLSGLAIILLWIGFGCGGHTTPRFSPFPGTITLTPST
                     TTSIQLGSVLPFTASARNAGNQIVNTAFTFASSDTSILNISPVGLACAGHWDASFTIC
                     TAGGVGVVQVIAEALGTSSPPTLVFVHPPIDNIIVAGILPPNQTVQEPCLSQSQTMTV
                     QAQAFSQGQDVTATVGPFTFTANNPGVVTLTPLLNDTVINGTRYNIATNQATATAATP
                     GIAQIFASASGVSSSSFRQPQYQKTVNGSVQTSPLLDFFETCPIQSIQVELGAAGSKL
                     TSFEVAKGSAAQTAVATLTDVMGNSSLPNNTGAIVLSKIPLTWTSSQTAVVNPPASCG
                     QTCSLAAANPGAAAITASCSPPTCNIGFPKIPTSLSTPQLLAQCAAFFQLASCQQFIP
                     APVYSSPTTPLLPNSTGSISAVVTGTAPAATVLATSTGCATTSPTDCTTSLYSVSTSN
                     AAPSAPSASPTPPNSLLFDLAGDKAFVGSNFGPVTVTTSSIGSGTSSTFTPLGAFTGS
                     ALAVSRTGTMAVFTSPDQVFVTNSSGQSVTALNIFNASAAAFSPDGLKAFIFGFDSNN
                     NPNLYVYSTQQALQVINLPPKTTVNSIAFSANGAFAYVVEPSLGAGGAAVSVYNTCNN
                     LPAQNSGGINQVIPLSEPPIAFRALPDGVNFIALHSDGTFDRFSATVAPVTAATLSQP
                     ATSALCPLRVSHKPLPPMSLNQGPIHAMNFFVSPDGTLIYVIASDRASILIFNVTTQA
                     VSSAIPLVNDATPVSADMSVDTATVAVAGSDGFLHLITTANGGNDNNQVSFPILPNFS
                     NPFCNFTPQGGPCTLNFAAVKP"
     gene            4144..4521
                     /gene="rpsL"
                     /locus_tag="VEJ00_02410"
     CDS             4144..4521
                     /gene="rpsL"
                     /locus_tag="VEJ00_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HXX00040.1"
                     /translation="MPTFNQLVAQGRKRPRYKTASPALQGCPQKRGVCTRVYTQTPKK
                     PNSALRKVARVRLTNGIEVTTYIPGVGHNLQEHSIVLIRGGRVKDLPGVRYHVIRGTL
                     DAVGVANRQQGRSKYGAKRAKKG"
     gene            4532..5002
                     /gene="rpsG"
                     /locus_tag="VEJ00_02415"
     CDS             4532..5002
                     /gene="rpsG"
                     /locus_tag="VEJ00_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HXX00041.1"
                     /translation="MPRKGHIAKRTVPADAVYGSDLVTKFINSMMWQGKRSTAEGIFY
                     EALTKLESKGGDTALKLFTKAVENTKPLLEVKTRRVGGANYQVPVEVNADRRTSLAIR
                     WIITYARARGEKGMIDKLSNELLDAANSRGAAIKKKEDVHRMAEANKAFAHYRW"
     gene            5110..>5910
                     /locus_tag="VEJ00_02420"
     CDS             5110..>5910
                     /locus_tag="VEJ00_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXX00042.1"
                     /translation="MPRTVPLERCRNIGIMAHIDAGKTTTTERILFYTGRTHRIGEVH
                     EGTATMDWMEQEQERGITITSAATTCTWRDIRINIIDTPGHVDFTAEVERSLRVLDGA
                     VAVFDAVHGVQPQSEKVWRQADKYGVPRICFINKIDKMGADFEHAVDTIRKRLSAKPV
                     AIQIPIGQEDKFKGVVDLLAMKAIVWKDDTMGAEYVTEEIPADLKKKAEAFHAQLVET
                     VAENDDEILHKFLEGEEISADELRKSLRKSTIELKLFPVVVGTAFKNKG"
BASE COUNT         1238 a         1624 c         1961 g         1087 t
ORIGIN      
        1 cgggccgcgt cccattagct tgggctcgac gccggcgatg ccccaggcga cgatccgccc
       61 cagaggctcg atctggcgct tgcgggcttc ctcgattgcc atgaccacca cggccgctgc
      121 gccatccaca atgccgctcg catttcctgc cgtcacggtg ccgttcttgc cgaacgccgg
      181 cttcagcttc gccagcccct ccatcgtggt ctgtggacga cggtggtcat cttcggtcag
      241 ttgttcgccc gtcggctcgc ctttggaatt ccggagcggc acaggaacca gttcttcccg
      301 gatgcgccct tctgtgtacc ccgcgtcggc gttctgctgc gagcgcagtg cgaactcatc
      361 ctgcatctgg cgggtgatgc cttgttcgga ggcgtacagt tcggcggtgt tggccatgta
      421 agaaccacaa tacgagtcca gcagcgccac catcagcgag tcctcgagct tgccgccggg
      481 agcgagcgtg aacgcgtcgc gtccgcggat cacaaaaggt gcctgcgaca tggcctccat
      541 gccgccggcg agcacgatct tcgcctcatc ggtctggatc atctgcgcgg cgctcacaat
      601 cgcctgcatg cccgagccgc acaaccggtt cacggtcaat gccggagtct caataggtat
      661 ccccgcccgc aatcccacgt gccgcgcgcc atacaacgcg tcgcccgagg tctgctgagc
      721 gtttcccatt accacgtgat cgaattcctt cgcgtcgatc ccggagcgct cgatcgcgcc
      781 cttggcggca atcgcgccca actcctgcgc ggtgaagtct ttcagcttgc cgcaatagcg
      841 gccaaagggc gtgcgcgccc cgctgacaat cgcaatatct cctggtttca gcatgatttt
      901 ctcgcagaac tccgcgcaga atcggccaga gtcatttcgt caagctagtc agtttagcag
      961 cgcgtgagga gattgaccac aaagtccggc cacgctcttg ggttctgtcc ggcaagtcgc
     1021 tgtcgttggt cgcctcgtgg gagctacggt ttcaccgccg cgaaattcaa ggtgcatggg
     1081 ccgccctgcg gcgtgaagtt gcagaaggga ttgctgaaat tgggcaggat ggggaaagag
     1141 acctggttgt tgtcgttgcc gccgttggca gtcgtgatca ggtgcagaaa gccgtcactg
     1201 ccggcgacgg cgacagtggc ggtatcaacc gacatgtcag cagagacggg agtggcgtcg
     1261 ttgaccagag gaatcgcgga gcttacggcc tgcgtggtga cattgaagat caggatgctg
     1321 gcgcgatcgc tggcaatgac gtagatcagc gtcccgtccg gcgacacaaa gaaattcatg
     1381 gcatggatcg gcccctggtt cagactcatc gggggcaggg gtttgtggct tacccgcagc
     1441 gggcagaggg cggaggtggc cggctggctg agggtcgcgg cggtgaccgg ggcaacggtg
     1501 gccgagaagc gatcgaaggt tccgtcgctg tggagggcga tgaaattcac cccatcgggc
     1561 aaggcgcgga acgcaatcgg cggctcggat aaaggaatga cctgattgat gccgccggaa
     1621 ttttgcgcgg ggagattgtt gcaggtgttg taaacggaaa cggcggcgcc gccggcgccg
     1681 agcgacggct ccacgacata ggcgaaggcg ccgttcgccg agaaagcgat cgagttgacc
     1741 gtggtcttcg ggggaaggtt gatcacctgc agggcctgct gggtcgagta gacgtagaga
     1801 tttggattgt tgttgctgtc gaatccaaag atgaaggcct tcaatccgtc gggagagaaa
     1861 gcggctgccg acgcgttgaa gatgttcagc gcggtgaccg attgtccact ggaatttgta
     1921 acgaagacct ggtcgggcga ggtgaacacg gccatggttc cggttctgga gacggccagc
     1981 gcgctgccgg tgaaagctcc gagaggcgtg aaggtgctgc tggtaccgct gccaatgctc
     2041 gacgtggtga ctgtcacggg gccgaagttg ctgcccacga acgccttgtc tccggccagg
     2101 tcgaaaagca aagagttcgg gggagtgggg ctggcactgg gagcgctggg ggcagcattg
     2161 ctcgtcgaga cgctgtagag cgacgtcgtg caatccgtgg gagaggtcgt ggcgcaccct
     2221 gtactggtgg ccagaacggt tgcggcgggc gccgttccgg ttaccacggc tgagatcgat
     2281 cccgtcgagt tgggcagcaa gggagtggtg ggtgaggagt acaccggcgc cggaataaat
     2341 tgctggcaac tggcaagctg gaagaaggcc gcgcactgag ccagcaactg cggcgtcgac
     2401 agtgatgtgg gtattttcgg gaacccgatg ttgcaggtcg gtggcgagca cgaggcggtt
     2461 atggcagcgg cgccggggtt ggccgcggca agactgcagg tctgcccgca ggaagccgga
     2521 ggattcacga ccgccgtctg ggacgaagtc caggtaagcg ggattttgct gagcacgatc
     2581 gcgccagtgt tgttgggcag cgaactgttt cccatgacgt cggtgagagt ggcaactgcg
     2641 gtttgggcag cgctgccctt ggcgacttcg aagctggtca gctttgatcc ggccgcgcct
     2701 agttcgacct gaatgctctg aatggggcag gtttcgaaga aatcaaggag cggggaagtc
     2761 tggacgctcc cgttgacggt cttttgatat tgaggttgcc ggaaagaact gctggagacg
     2821 ccgctggccg acgcgaagat ctgggcgatt ccgggagtag ccgcagtcgc cgtcgcctgg
     2881 tttgtagcga tgttgtaacg cgtcccgttg atgacggtgt catttaagag aggagtgagg
     2941 gtcacgacgc ctgggttgtt cgcagtgaaa gtgaaagggc caaccgtagc tgtaacgtcc
     3001 tggccctggc tgaatgcctg ggcctgcacg gtcatggtct gcgactggga gaggcaaggt
     3061 tcctgaacgg tctggtttgg cggcagaata ccggcaacaa tgatgttgtc gatcggagga
     3121 tgcacgaata ccaaggtagg cgggctcgaa gtgccaaggg cctcggcgat gacctgaacg
     3181 acgcctactc cgccggcggt gcagatggtg aagctggcgt cccagtggcc ggcacaggcg
     3241 agaccgacgg gcgatatgtt gaggatggaa gtgtcgctcg atgcaaaagt gaatgccgtg
     3301 ttaacgatct ggttgccggc gtttcgagcc gacgctgtaa atggcagaac gctgcccagt
     3361 tggatcgacg tggtggtaga aggcgtcaga gttatcgtcc cggggaaagg cgagaagcga
     3421 ggcgtggtgt ggccgccgca tccaaaaccg atccacagga gaatgatggc caatcctgac
     3481 aagctaccgg aacgcaacca gcgcatgcac ccctcttaag cccgggtccg gcaccgcatc
     3541 ccgacaaggt gtggtgccaa gccgccgcat catgaagtgg aaaacctgta gtgtagaggc
     3601 tgaaggcgga atcagcaagg gtcgcggccg ggataaccgg aaccgtttcg ccaaaacgcc
     3661 agtagttggg atgcgaaaag acgggttgac gatgtttcga tgcgcttgct atagtatttt
     3721 ggttccgcga gttcatccag gcttgttgtg cgctgcgggt ttcttgcgcg ttgatgcgcg
     3781 cagcgaacca gtgcagtcga gcttcagaga aatacgtttc cggaaaggcg ttgcgaccgg
     3841 aagctttcct cgctgaaacg tgcctgcggt gccttccgcc ctattgtgga aggatatgca
     3901 gggcaaggct gacgaaacgg agtgcccccg gggtttggcc gggcgccacc gtcggcagcg
     3961 taagtctgaa tgggaatcca gaatctctct ggcgttggct tgccgtaact gagctgcgcg
     4021 gtgagaagta atggtcggca tcgcgccggt catgcccacg gatgcgcagg cagcgccgcg
     4081 ggatcaggcc cagcaggagc aagctgtgcg gcgcgaacaa gcgccgctaa ggagtggcga
     4141 agagtgccta cgtttaacca gttggttgct cagggccgca aacggccccg gtacaaaacg
     4201 gccagcccgg cgttacaggg atgtccgcag aagcgcgggg tgtgcacccg cgtgtacacg
     4261 caaacgccga agaagccaaa ctccgcgctg cgcaaagtgg cacgcgtacg cctgacgaat
     4321 ggaattgaag tcacgacgta tatccccggc gttggccaca acctgcagga gcactcgatt
     4381 gtgctgatcc gcggaggccg cgtgaaggac ctgccgggcg tgcgctatca cgtgattcgc
     4441 ggaaccctgg acgcggtagg cgtcgccaac cgtcagcagg ggcgctcgaa atacggcgcc
     4501 aagcgggcaa agaagggctg aggactgatt catgccacgc aaaggacata tcgcgaagcg
     4561 gaccgtgcca gccgatgccg tgtatggctc ggatctggtg acgaagttca tcaactcgat
     4621 gatgtggcag ggcaagcgct cgaccgccga gggcatcttc tatgaagcct tgaccaagct
     4681 ggaatcgaag ggcggcgaca cggcgctgaa gttattcacg aaggcggtgg agaacacaaa
     4741 gcccctgctc gaggtgaaga cgcgccgcgt tggtggcgcg aactatcagg tcccggtcga
     4801 ggtaaatgcg gatcggcgaa cttcgctggc gatccgctgg atcattactt atgcacgggc
     4861 ccgcggcgaa aagggaatga ttgacaagtt gagcaacgag ttgctggacg cggccaacag
     4921 ccgcggcgct gccatcaaga agaaagaaga tgtgcaccgc atggcggaag ccaacaaggc
     4981 gttcgcgcac tatcggtggt agagaaaccg gctcttggcc tttagctctt ggcttgaacc
     5041 agggccggtt gagccaatag ccaagagcca aaagcgaact aacggagccc cagaaagtag
     5101 aacaacgtcg tgcccagaac agtcccatta gaacggtgca ggaacatcgg catcatggcc
     5161 cacatcgatg ccggcaagac cactacgacc gagcgcatcc tgttttatac gggcaggacg
     5221 caccgcatcg gagaggtcca cgaaggcacc gccaccatgg actggatgga acaggagcag
     5281 gagcgcggca ttacgatcac ctccgccgcg acgacctgca cctggcgcga tatccgcatc
     5341 aacatcatcg atacacccgg ccacgttgat ttcacagccg aagtcgagcg ctcgctgcgc
     5401 gtgctcgacg gcgcagtcgc cgtatttgac gcggtgcacg gcgtgcagcc gcagtcggaa
     5461 aaagtatggc gacaggccga taagtacggc gttccgcgga tttgtttcat taataagatc
     5521 gacaagatgg gcgccgattt cgagcatgcc gtcgatacca tccgcaaacg cctcagtgcc
     5581 aagccggtcg cgattcaaat ccccatcgga caagaagaca aattcaaagg cgtcgtcgat
     5641 ctgctcgcca tgaaagcgat cgtgtggaaa gacgacacca tgggcgccga gtacgtgacc
     5701 gaagaaatcc ccgccgatct caagaagaag gccgaagcct tccacgctca gctggtcgaa
     5761 actgtagccg agaacgacga cgaaatcctg cacaagtttc tggaaggcga agagatctcg
     5821 gccgacgagc tgcgcaagtc gttgcgcaag tcaaccatcg agttgaagtt gtttccggtc
     5881 gtggtaggca cggcgttcaa gaacaaaggc
//
LOCUS       DASPUL010000104         3886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_975293, whole genome shotgun sequence.
ACCESSION   DASPUL010000104 DASPUL010000000
VERSION     DASPUL010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975293"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VEJ00_02425"
     CDS             <1..620
                     /locus_tag="VEJ00_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00043.1"
                     /translation="AIWYASRASAVAPPQAQAQIERYARSLYVRFHGGDDGWADVLAK
                     AKAGGPVPDLATLIKPAPTPAEQAHKMVQETAPDKMDFATWEFVLTNGSQEDQDAVWN
                     AIKGKAVQMVGTVISTQPDTFMVAGSSDDIDAKKADITLKFEDKIPPRMIPKEGTDNF
                     QFQGEPASYTPNPFMMVMEKGRLLNAKPAAPAHKPVTHHKPAASQ"
     gene            complement(823..1101)
                     /locus_tag="VEJ00_02430"
     CDS             complement(823..1101)
                     /locus_tag="VEJ00_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXX00044.1"
                     /translation="MKDQLEALVLQMYRSNILYAEGVREFKKRFILTVLQENKGNQCR
                     AARELGMHRNTLSRTINELKIDVRQLRDGGKRPPRSARPVTVEKKVVR"
     gene            complement(1140..1700)
                     /locus_tag="VEJ00_02435"
     CDS             complement(1140..1700)
                     /locus_tag="VEJ00_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HXX00045.1"
                     /translation="MSPADWMICALVLVNVVAAAIQGFFSEALTIAGLVVGYLVAAWQ
                     YRSVAEWLESFLKNPWLAEILGFLIIFFAIVLVFGIAARIARWIMKHAGLSGFDRFLG
                     GLLGLLKGGLMVAVILMGMTAFAPTSKFLANSQLAPYFLVVGRAAIWLAPSELRARFY
                     QGLDLLHQAPQKLTGAPSSPPKPSSK"
     gene            complement(1740..2651)
                     /locus_tag="VEJ00_02440"
     CDS             complement(1740..2651)
                     /locus_tag="VEJ00_02440"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXX00046.1"
                     /translation="MTPELLDPVLIQTDFPDLPIHASGKVRDVYSLGPEQLLFVATDR
                     ISAFDYVLATGIPHKGRVLTQLSLFWFDFLADIVPNHLITADVGQYPASVRKYAEQLR
                     GRSMLVRRAEMFPVECVVRGYISGSAWKEYKATGKVSGISLPAGLKESDAFPEPIFTP
                     STKATTGHDENISFDQMCDIVGVETASTLRDLTLRVYKKAAAYARQRGIIIADTKFEF
                     GRTAQGITLADEVLTPDSSRFWPADKYAPGRAQDSYDKQYVRDYLEQIRWNKQPPAPA
                     LPADVARRTSEKYLEAYFQLTGHRLDV"
     gene            complement(2897..>3886)
                     /locus_tag="VEJ00_02445"
     CDS             complement(2897..>3886)
                     /locus_tag="VEJ00_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00047.1"
                     /translation="AAEKLQRETENRQLQERATELEAERNAAQAREGLLQFETEQLRA
                     RLTEIDDLLRNSRLLLDQARDRRGELSATAAKLQSDAQYMSETCLNELGVESTALMSD
                     TTLAMLTGDELATEDQIYRDMRAKLEAMGPVNMMALEEYKETAERHTFLETQRQDLIA
                     SIENTTATIREIDQISRQKFEEAFNKINENFQATFKKLFGGGHAFMKLTDELNSAESG
                     IDVVASPPGKRLQNVLLLSGGEKALTALSLLVGIFQYAPSPFCILDEVDAPLDEANIG
                     RFTELVREMSVQTQFVLITHSKKTMSIAPVLYGVTMQEPGVSKLVSVRFGAQA"
BASE COUNT          813 a         1105 c         1187 g          781 t
ORIGIN      
        1 atgcgatatg gtacgcgtcc cgcgcctctg ccgttgcacc gccacaagct caggcccaga
       61 tcgaacgata tgcgcgttcg ctatacgtga gattccacgg aggagacgac ggctgggctg
      121 acgttctggc gaaagccaaa gcaggcggtc cggtgccgga cctggcgacc ctgatcaagc
      181 ccgcgcccac tccagccgag caggcgcaca agatggtgca ggagacggct cccgacaaga
      241 tggactttgc tacgtgggag ttcgtgctga ccaacggcag ccaggaggat caggacgcag
      301 tttggaatgc gatcaaaggc aaagcggtgc agatggtggg caccgtcatc agtacccagc
      361 cagatacatt catggttgcc ggcagttcgg atgatatcga tgccaagaaa gcggacatca
      421 ccttgaaatt cgaagacaag attcccccga ggatgattcc caaggaggga accgacaact
      481 tccagtttca gggtgagccg gcgtcctata ccccgaatcc cttcatgatg gtcatggaga
      541 aggggcggct gctgaatgcc aagccggcag ctccagcgca taaaccggtg acgcatcaca
      601 agccagcagc gtcgcaatag cgttcccgat cgttattacc tgagaaggcg gccctgagag
      661 gccgcctttg tttctcttga cctgaaaaac gtgggttcat ggcgggaact ctcttctgag
      721 gccgcctatc atgcagactc aaaggcatgc ttcgactggg ctgcgaaatt cacttcgtga
      781 attccgcagc ttgcccgata tgacaaggga caggggcgcg gtttagcgga cgactttctt
      841 ctccaccgtg acggggcggg cgctgcgggg cgggcgcttg ccgccgtcgc gcaactggcg
      901 cacatcgatc ttcaactcat taatagtccg gctgagcgtg ttgcggtgca tgcccagttc
      961 acgggcggcg cggcactgat tgcccttgtt ttcctgaagc acggtcagga tgaagcgctt
     1021 cttaaactcc cgcactccct cggcataaag aatgttgctt ctgtacatct gcaggacgag
     1081 tgcttctaat tgatctttca cggtggaatc tctctcgatg aattttcact gcacttcgcc
     1141 tacttcgagg acggcttcgg cggggaagat ggggcgccgg tcagcttctg cggagcttga
     1201 tggagcaggt ccaaaccctg atagaagcgg gcgcgcaact ccgaaggcgc cagccagatg
     1261 gcggctcggc ccaccaccag gaagtaaggc gccagttgcg aattggccag aaacttcgaa
     1321 gtgggcgcga acgccgtcat acccatcagg atcactgcca ccatcaatcc gcccttcagc
     1381 aaccccaaga gtccgccgag gaaacggtca aacccgctga gcccggcatg tttcataatc
     1441 cagcgtgcga tcctggcagc gatgccaaaa accagcacga tggcaaaaaa aataatcaga
     1501 aagccgagga tctcagccaa ccacgggttc ttcaaaaacg attccagcca ctctgccaca
     1561 ctgcgatact gccacgccgc cacgaggtag ccgacgacca gtcccgctat ggtcagggct
     1621 tcggaaaaaa aaccctgtat agccgcggcg accacgttca cgagcaccaa cgcgcaaatc
     1681 atccagtccg ccgggctcat acggaacacc tgcgggtgga gatggtacca gacgccgggt
     1741 cagacatcca gcctgtgccc ggtcaactga aaatacgcct cgaggtattt ctcgctggtg
     1801 cgccgggcga cgtccgcagg gagcgcgggc gccggcggct gcttgttcca gcggatttgc
     1861 tcgaggtagt cgcgaacata ctgcttgtcg tacgaatcct gcgcccggcc cggagcgtac
     1921 ttgtcggcgg gccagaagcg ggatgaatcg ggagtcagga cttcatcggc caaggtgatg
     1981 ccctgggcgg tgcgtccgaa ctcgaacttg gtgtcggcaa tgatgatgcc gcgctggcgg
     2041 gcataggccg cggccttctt gtacacgcgc agcgtcagat cgcgcagggt actggcagtt
     2101 tcgacgccga cgatatcgca catctggtcg aaggaaatgt tttcgtcgtg gccagtcgtg
     2161 gccttcgtcg aaggcgtaaa aatgggctcg ggaaaagcgt cggattcttt caagcccgcg
     2221 ggaaggctta tccctgagac tttgccggtc gctttatatt ccttccaggc cgaaccggag
     2281 atgtacccac gcaccacgca ttcgaccggg aacatctcgg cgcgtcgtac cagcatggag
     2341 cgtccgcgca actgttcggc atatttgcgg accgaagccg ggtattgacc gacgtcggcg
     2401 gtaatcagat ggttcgggac aatgtcggcc aggaaatcga accagaacag cgaaagctgc
     2461 gtgagcacgc gtcccttgtg agggatgccg gtggccagaa catagtcgaa ggccgagatg
     2521 cggtcggtgg cgacaaaaag aagttgttca ggaccgagcg agtagacgtc gcgtaccttg
     2581 ccgctggcgt gaatcgggag atcgggaaag tcagtttgga taagaacggg atcgaggagt
     2641 tcaggagtca tgatgaaatt ctacaggcga gcgaggcgta ttctagctgc tcagggaaaa
     2701 atgtcaacga ttctcgtcac tcgggacgga cgcagtttcc gaccgtccaa gggaaagaag
     2761 ttgacaagac gcgcaatggc cgggaacgta gacgggacaa ggacttccgg ctggtagagt
     2821 tgtggaaaag ctgaggatcg gagtggatat cagggaaagt tccccacatc ccatcttgag
     2881 cctcgcagcg tctatctcac gcctgcgcgc cgaaccgtac cgagaccagc ttggagactc
     2941 ccggctcctg catggtgact ccatagagca cgggcgcgat gctcatcgtc ttcttgctgt
     3001 gggtgatgag cacgaactgc gtctgcacac tcatctcgcg caccagttca gtgaagcggc
     3061 cgatgttggc ttcgtcgagc ggggcgtcga cttcgtcgag gatgcagaag gggctgggcg
     3121 cgtactggaa gatgccgacc agcagcgata gtgcggtcag cgccttttcg ccaccggaaa
     3181 gcagcagcac gttctgcagg cgcttgcccg gaggcgaggc cacgacgtcg atgccgcttt
     3241 cagcgctgtt cagttcgtcg gtcagcttca tgaaggcgtg gccgccgccg aaaagcttct
     3301 taaaagtagc ctggaagttc tcgttgatct tgttgaaggc ctcttcgaac ttctggcgtg
     3361 aaatctgatc gatctcccga atcgttgcgg tcgtgttctc gatcgaggcg atcaagtctt
     3421 gccgctgagt ttcgaggaaa gtgtggcgct cggccgtttc cttgtactcc tccagagcca
     3481 tcatgttcac cgggcccatg gcttcgagct tggcgcgcat gtctcggtag atctggtctt
     3541 cggtggcgag ttcgtcacct gtgagcatgg cgagcgtggt gtcgctcatg agtgccgtgc
     3601 tctcgacccc gagctcgttc aggcatgtct ccgacatgta ttgcgcgtcg gactgcagtt
     3661 tcgccgcggt agccgacaat tctcccctcc gatcgcgggc ctggtcgagc aacaggcggc
     3721 tgttgcgcag cagatcgtcg atttccgtga gccgggctcg cagttgctcg gtttcaaact
     3781 gcagtaatcc ttcgcgggcc tgagcggcat tgcgctcggc ctcgagctct gtggcgcgct
     3841 cctgcagctg cctattttcg gtctcgcgtt gcagtttctc agcggc
//
LOCUS       DASPUL010000105         5820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1660713, whole genome shotgun sequence.
ACCESSION   DASPUL010000105 DASPUL010000000
VERSION     DASPUL010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5820)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5820
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660713"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VEJ00_02450"
     CDS             complement(<1..332)
                     /locus_tag="VEJ00_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00048.1"
                     /translation="MTTRKLWAWIILLASIAFFLQASIVARAQDEDEDQSQDPPGRVA
                     RLDYSQGSVSFRPAGEDDWVTGVPNRPLVTGDDLWADENSRAEAHIGSTAIRLGQKTG
                     ITFLALDDR"
     gene            711..896
                     /locus_tag="VEJ00_02455"
     CDS             711..896
                     /locus_tag="VEJ00_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00049.1"
                     /translation="MRNYLRFFAVLVVGLAAYVVLLHSFHLMNEAGDHAFYGGIAEIL
                     GVILFVPLVVRAIWRNL"
     gene            complement(1106..1564)
                     /locus_tag="VEJ00_02460"
                     /pseudo
     CDS             complement(1106..1564)
                     /locus_tag="VEJ00_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            1865..3661
                     /locus_tag="VEJ00_02465"
     CDS             1865..3661
                     /locus_tag="VEJ00_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXX00050.1"
                     /translation="MSIATLNDIFFAAVERNLDRMMLYREEGKWQPISSRQFGQRVAR
                     LACALQDWGIRPGDRVAILSENRPEWPIADMASLLLGAVTVPLYATLTAEQSAFILND
                     AACRIMFVSSDQQLHKILSIVSETGIEKLIVMDPVALTGDLERSAGRWITLDKFCEQQ
                     ADDLTAAMEARARGIGPDDLATIVYTSGTTGTSKGAMLTHGNIASNIQCSLMGFNMRP
                     GLVSVSFLPLSHITARHVDFALLCHGVTLAYCPFLDRLPASLLEIQPTIFVGVPRVYE
                     KIYAQAEQKAKGFPKRAIFDWALRVGQRNKPEILAGKTPTSLSWKLADKLVFSKIREG
                     MGGQVGTFISGGAPLGRELAEWYATVGIRIHEGYGLTETSPVIAVNNPVHHRIGTVGK
                     ILANLEVRIAEDGEILVHGPSVFKGYWNRPEETRASLNDGWFKTGDIGNIDADGYLSV
                     TDRKKDLIKTSGGKFIAPQPIENALKLNSLIGVAAILGDKRKFPAVLISPNFPLLEDW
                     ARTNGIPFSSRTELVANPKVQALYEAVIEDINQNLARFEKLKRVLLVADEFTADNGVL
                     TPTLKLRRRVVEERYRRQINELYAQAEAAPVQ"
     gene            4137..4910
                     /locus_tag="VEJ00_02470"
     CDS             4137..4910
                     /locus_tag="VEJ00_02470"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HXX00051.1"
                     /translation="MTDSLIVAGRSFRSRLIVGTGKYKSFQETARALEASGAEMVTVA
                     VRRVNLDRSKESLLDYIDPKKYFLLPNTAGCYTADEAIRAARLGREVGLSDWVKIEVI
                     GDQATLYPDVQGTLEATRVLVKEGFTVLPYTSDDVVVAKRLVDAGAAAVMPLGAPIGS
                     GMGIQNEANIRILREMITGVPLIVDAGVGTASDAAIAMELGADAVLMNTGIAAAQEPV
                     LMAEAMKHAVIAGRQAYLAGRMAKKLYATASSPLEGVVR"
     gene            complement(5044..5373)
                     /locus_tag="VEJ00_02475"
     CDS             complement(5044..5373)
                     /locus_tag="VEJ00_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00052.1"
                     /translation="MRKVLLICLAVCFLLAFTAFAFDDMGKSATVNGWVVDDKCGVKG
                     AHAGAEECTKKCLAAGAKMVIVTDGDQKVLKVDNPDALKGHEGHHIAATGAVKGDSIH
                     VDSMKML"
     gene            complement(5570..>5820)
                     /locus_tag="VEJ00_02480"
     CDS             complement(5570..>5820)
                     /locus_tag="VEJ00_02480"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXX00053.1"
                     /translation="GANGMIVYKQKRAEYEKLRRQINQVQDDNDRYTQQIQGLKSDQT
                     AIEKEAREQLGYAKPGEYVYVAPAVVKPEPPSNHSARK"
BASE COUNT         1144 a         1695 c         1767 g         1214 t
ORIGIN      
        1 cgatcgtcga gagcgagaaa agtgattccg gttttctggc ccagacggat ggccgtcgat
       61 ccgatatgag cttcggcgcg cgaattctcg tcagcccaga ggtcatcgcc ggtgaccaaa
      121 ggccggttgg ggacgccagt cacccagtca tcttcgccgg ccgggcggaa cgaaaccgag
      181 ccctgcgagt agtccagccg cgcgacgcgg ccggggggat cctggctctg atcctcatct
      241 tcgtcctgcg ctctcgcgac aatcgacgcc tgaaggaaga atgcgatgct cgccagcaga
      301 atgatccatg cccacaactt gcgtgtcgtc ataaagtacg cccctgagcg tgtggatttg
      361 gtgtacttcg atcttaccct caggcgatta aacacggtag ggccggttta gtttccggcc
      421 agctctcccc cgaacgtaag taaccgttgc aaatcccagc gggaaaaggg ctttccggtt
      481 ttgaatccgc ccggtggaga gggcatcgaa ttaggtgagc agttcgaggg caggttccag
      541 ccaaacttgc tctgttcaca tgtaaagcac ctccaacgcg gcttcctttt gtagcggtat
      601 ctcgaatccc ctaatattca aacgagaatt ccgaacagga atattccgca gagtttgccc
      661 gggcggtttt gcgcgggcaa ggacgggctc cagcgccagg agtgtgtccc atgagaaatt
      721 acctgcgttt ctttgcagtg ctggtggtcg ggttggcggc ttacgttgta ttgctgcact
      781 cgttccactt gatgaatgag gccggcgacc acgcgttcta tggcgggatc gccgagattc
      841 taggcgtgat cttgtttgtt ccgctagtgg ttcgcgcgat ttggaggaac ctatgactat
      901 ctttgacaac gaccgggtca tttccagttt cctggtgcgc gcgacccgca tcgagggcga
      961 ggcgtcatcc cgaagccccg cgctattacc agcggggcga gggatctcct cttgacgggt
     1021 ctgtggatcg ggagatccct cgctccgcct gaaaagcggc tccgctcggg atgacgccgc
     1081 tactaagacc ttggggcggc tgcgcttaat tcttcagctt gggtctggca caggcatcga
     1141 cgatcttctt agggtcgatc gcgtcccggt cgacctgctc ttccatgatc ttgccatcct
     1201 tgccgatcag gaagttcacg cggcgcgggg cctggtattc gtctttgtag attccgtact
     1261 cgtgggtcac tttgccgccc cagtcactca acagtgggaa ggtcactccg atctggtcgg
     1321 cgaaagcttt gttggcaaag gcactgtcca tgctcacacc cagaacctgg gtgtcggcgg
     1381 cttccagggt tttcaggttc gcctggaagg cctgcatctg cttggttcaa ccaccggtga
     1441 aggcgaagat gtagaacgcc accacgacat ttttcttgcc gcggtagtcg ctcagcttta
     1501 catctttcag gtcattgccg tcgaagtact tcagcgtgaa atccggagcc atatcgccca
     1561 ccttgggcat ggccgaaccc tccttgctct gggcgtacag cgcgggtgat gggttcagca
     1621 tgccgctgaa cgtccccgcg agaagcaatc ctgccgccag aacccctgag cgtttcttta
     1681 gcattgaact ccccccatcg gagtggtacc cagcatatcg ccgcggggca tttggggtaa
     1741 acccggaagt acccaaggga tgtgattcta cgcattgccg aaaccgtggg cgagacatta
     1801 catttttgtt atccgtgagc tgcacggagc gagagctgcc acgaactcct ccccaaacac
     1861 cgtgatgagc attgccacgc tgaatgacat tttctttgct gccgtcgagc gcaatctcga
     1921 ccgcatgatg ctctatcgtg aggaagggaa gtggcagccg atcagctcgc gccagttcgg
     1981 acagcgcgtg gcgcgactgg cgtgcgcgct gcaggattgg ggtattcggc cgggggaccg
     2041 cgtggcgatc ctcagcgaaa accgtccgga gtggccgata gccgacatgg ccagcctgct
     2101 gctgggcgcg gtaaccgtgc cgctttatgc gactctcacc gccgagcaaa gcgcattcat
     2161 ccttaacgat gcggcctgca ggatcatgtt tgtttcatcg gaccagcagc tgcacaagat
     2221 actctcaatc gtctccgaga cggggatcga gaagttgatc gtgatggatc ccgtggcact
     2281 cacgggcgat ctggaacgct ctgccggacg ctggatcacg ctcgacaaat tctgcgagca
     2341 gcaagcggac gatcttactg cggcgatgga ggcaagggca cggggcatcg gtcccgacga
     2401 tctggcgacc atcgtttaca cctcgggaac gacaggaacc tccaagggtg cgatgctgac
     2461 ccacgggaac atcgcgtcca acattcagtg ctcgctgatg gggttcaaca tgcggccggg
     2521 actggtcagc gtctcgttcc ttccgctcag ccacatcacc gcgcgccacg tggatttcgc
     2581 gctgctctgc catggcgtga cgctggctta ctgccccttt ctcgaccggc tgccggccag
     2641 cctgctggag atccagccga ccatttttgt aggcgtgccc cgagtctatg aaaagatcta
     2701 cgcccaggcg gagcagaaag ctaaaggctt ccccaagcgt gcaatcttcg attgggcgct
     2761 gcgcgtaggg caacgtaaca agcccgagat tctcgcgggg aagactccca cgtcgctcag
     2821 ctggaagctg gctgacaagc tggtattctc gaagattcgc gagggcatgg gcggacaagt
     2881 gggtacgttt atttccggcg gggcgccgct agggcgcgaa ctggccgagt ggtacgcgac
     2941 ggtcggcatc cgcattcatg aaggctacgg actcaccgag acgtcgccgg tcatcgccgt
     3001 gaacaatccc gtgcatcatc gcatcgggac agtgggcaag atactggcga atctggaagt
     3061 gcgcattgcc gaggatggcg aaatcctggt gcacggaccg tcggtcttca aaggctattg
     3121 gaatcgtccg gaagagaccc gggcgtcttt gaacgacgga tggttcaaga ccggcgacat
     3181 cggcaatatt gacgccgatg gatatctctc ggttaccgat cggaagaagg atctgatcaa
     3241 gacatccgga ggaaagttca tcgcgccgca gccgattgaa aatgcgctga agctcaattc
     3301 cctgatcggg gtggcggcga tcctgggcga caagcggaag tttcccgcgg tactgatttc
     3361 gcccaacttc cccctgctgg aggattgggc acggacgaac ggcattccat tctcgtcgcg
     3421 cacggaactg gtggcgaatc cgaaggtaca ggcgctgtat gaagccgtga tcgaagacat
     3481 caaccagaac ctggcgcgct ttgagaagct gaagcgggta ctgctggtcg ccgacgaatt
     3541 taccgccgac aacggagtgc tgacgcccac cctcaagctg cgtcgccgcg tggtcgaaga
     3601 acgctaccgg cgacagatca acgagttgta cgcacaagcc gaagcggcgc cagtgcaata
     3661 gtcccaagac ccggatctcc tcatagtgtg tgcaagggtc gtgggaggat atgtgagcaa
     3721 gatgatatcc gcttgcggag tgctctgctc ggattgtcca gcctatctcg gcgccagcaa
     3781 gggatttgag caccagaagc ggaccgtggc cgcatggcga cggatctacg gagtcgagga
     3841 aacggccgag aacatctcct gtagtggttg tctcgggcca gatgatcagc tgtttcacac
     3901 cagccgcact tgcgaggcac gccggtgctg ccgatccaag ggtttcgcta cctgcgccga
     3961 ttgccccgtg gaagattgtc ccgctcttga aagagcgcag tccgtgtggg atggtgtgcc
     4021 gaaaataggc aaggccctgt ctagcaggga cttcgtgacc tacgctcgac cgtattgcga
     4081 tcaccgccga cgcctagccg aagcacgccg agcgcccaaa tgattacaat acctccatga
     4141 cggactcatt gatcgttgcc ggacgctcct tccgctcccg gctgattgtc ggcacgggga
     4201 aatacaaatc cttccaggaa accgctcgtg cgctcgaagc cagcggcgcg gagatggtca
     4261 cagttgcggt gcggcgagta aacctcgatc gcagcaagga atcgctgctc gattacattg
     4321 atcccaagaa gtattttctg ctcccgaaca ccgccggctg ttacacggcc gatgaggcga
     4381 ttcgcgccgc gcgtctgggc cgcgaggtgg ggctgtcgga ctgggtaaaa atcgaagtca
     4441 tcggagatca ggcgaccctg taccccgacg tccagggcac gctcgaggcg acgcgggtcc
     4501 tggtgaaaga gggctttacg gttctgccct atacatccga cgatgtcgtc gtcgccaagc
     4561 gtctggtcga tgcgggagcc gcggccgtga tgccgctggg cgcgccgatc ggcagcggca
     4621 tgggcattca gaatgaagcg aacatccgca ttcttcgcga gatgatcacg ggcgtgccgt
     4681 tgattgtgga tgccggagtg ggcacggcgt cggacgcagc gatcgcgatg gaactcggcg
     4741 ccgacgcggt gctgatgaac accggaatcg ccgcggcgca agaacccgtg ctcatggctg
     4801 aagcaatgaa acacgcggtg atcgccggac gccaggcgta tcttgccgga cgcatggcga
     4861 agaagttgta tgccacggcc agttcgccgc tggagggcgt ggtgcggtag gagtctgagg
     4921 cgccggtccc ggcgctttgc ggcgctgcgc gcctggacag ccgaggcggc tgtccctaca
     4981 tctgctgcta aaaatagggc ggcttttgac cgccctcgtc aggtccagct cgatcctaat
     5041 tcctcacagc atcttcatgc tgtccacgtg gatggaatcg cccttcaccg cacctgtcgc
     5101 ggcgatgtga tggccttcgt gtcccttgag cgcgtccggg ttgtcgacct tcaggacctt
     5161 ctgatcgcca tcggtcacga tgaccatctt cgcgccggcg gccaggcact tcttggtgca
     5221 ctcttcggcg ccggcatgcg cgcccttgac gccgcacttg tcgtcgacga cccacccgtt
     5281 gacggttgca ctcttgccca tgtcatcgaa ggcaaacgcg gtaaaggcaa gcaagaagca
     5341 gaccgcaaga cagatcagca gaacttttct catggccggt tattctcctt tttgaattcc
     5401 ggttgcggcc ccacgccgca acatagcctg tgacgatgac ggtccccgag caggaccaac
     5461 gcatctcgtc aattgggaat ttgtggcgac ggaactatac cacaggcgaa tgcagggttt
     5521 gtcagcgcac ccgggatcct tactcaaggc ttgccggaga aaattgcgtt cacttgcggg
     5581 cggaatgatt ggacggcggc tccggcttga ccacagcggg ggcgacatag acatactctc
     5641 ccggcttggc atagcccagt tgttcgcgcg cttctttctc gatggcggtc tgatcgctct
     5701 tcaggccctg gatttgctgg gtatagcgat cgttatcgtc ctgcacctga ttgatctggc
     5761 ggcgcagctt ctcgtattcg gctcgcttct gcttgtacac gatcatgcca ttcgcgccaa
//
LOCUS       DASPUL010000106         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1796818, whole genome shotgun sequence.
ACCESSION   DASPUL010000106 DASPUL010000000
VERSION     DASPUL010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1796818"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="VEJ00_02485"
     CDS             <1..1577
                     /locus_tag="VEJ00_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00054.1"
                     /translation="PLPLHRILHCRILYLAAWLVLLIAGVTGWAAVATAQEDLPQINP
                     GERKIPRKKDEGPRALALLKLTADGKASLLPITILINGKFWDATAYKADPVPMALDSG
                     TVYEAERTGSSLGLFTVATALHSKATNVLSPWIGTGAWAPAGSEKANTEHAAESVPVG
                     IDTSDQPPRLTKDPSRLRLPEETSPSSTKPASAPSSPGASSDEPPRLSKPSAPSSPPS
                     SPSPSSAPSGGSNPPGSSAPAPGAAPVGSSSPSASQSKAGDTKAEAKTPESDSGASDA
                     NRPRLRRGKPTAPLPDEEVAGYGKPGAEPAAGAAGASPAPANEGQFQLIPAISDAKGP
                     EPRPFTFEWLKDEEGERRQQLTTMAKDELRAYLDAQAKARIAPIATTTPKTTRHTPPA
                     KPPEPILENVRMNAYDLWVSNQPVIVFSAEAHVPATPTPSGQATPESNLQYSILLVTY
                     PDIYNNLRKLYVGVTDKYHLDITPKLQLVDAVDVDGDGRGELLFRETSDAGGGWVIYR
                     ASADKLWKMFDSLNPE"
     gene            1650..2582
                     /locus_tag="VEJ00_02490"
     CDS             1650..2582
                     /locus_tag="VEJ00_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXX00055.1"
                     /translation="MRKTSLLLALFLVSGLAAAQDQDFSKVQMKVTKVAGSVYMLEGA
                     GGNIGASVGDDGIVVVDDQYAPLAEKIQAALKGITDKPVRFVINTHYHEDHTGGNAYF
                     QKQAPIIAHDNVRKRLESGGTAGVGPGGSMHFEAKPQPKEALPIITFDHDVTVHLNGE
                     DIRALYFPAGHTDGDSVIFFPKSNVVHMGDDFVTYGFPFIDVDSGGSIDGMIDGVEKV
                     IAQVPADVKIIPGHGPLSNLDDVRAYLKMLKETRAAVAGALEKHQTLAQMKQAKLLDP
                     WKKYSGDFINEDAFLETLYNSLTGQPNGKFIKHN"
     gene            2633..>3746
                     /locus_tag="VEJ00_02495"
     CDS             2633..>3746
                     /locus_tag="VEJ00_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HXX00056.1"
                     /translation="MRLTRRRFVQSILGAGATAGLPALFSACTGSSRAGTSASGAVAA
                     RQFSGAVDAVFPRGFFWGAATAAYQIEGAWNEDGKGESIWDRFAHTPGKIKNGDTGDV
                     ACDSYHRWRDDIALMRAMHLNSYRFSISWPRIQPAGSGAANRKGVDYYSRLVDAVLEA
                     HIRPLVTLYHWDLPQALEEAGGWPNRDTAARFADYAQLVAQALGDRVSDWIIFNEPDA
                     FVDLGYLEGTHAPGHKSLLDFLRATHVVNLAQGAGFHALKAVRPSARVGTAFSMSPCE
                     PATESAEDKLAAERAHAISNIWFLEPALRGRYPEALTFLPETAMGVKSGDMEKTRAPL
                     DFIGVNLYYRTIASAPGTMERFAHAQDWLFPVKMDGG"
BASE COUNT          727 a         1164 c         1184 g          671 t
ORIGIN      
        1 accctctgcc gctccaccgg attctacact gccggattct atacctggcc gcgtggctgg
       61 tcttgttgat agccggggtc actggttggg cggcagtcgc gactgcgcaa gaggaccttc
      121 cgcagatcaa tcccggagag cgcaagatcc ctcggaagaa ggacgaggga ccgcgggcgc
      181 tggccctgct gaaactgacc gccgacggca aggcttcctt gctaccgatc acaattctga
      241 tcaacggcaa gttctgggac gcgaccgcct acaaggccga ccccgtgccc atggcgctgg
      301 attcgggtac ggtttatgag gcggagcgca ccggctcgtc gctgggcttg ttcacggtgg
      361 ccaccgcgct gcacagcaag gccacgaacg ttctcagccc gtggatcggg accggggcct
      421 gggctccggc ggggagcgag aaggcgaata ccgagcacgc ggccgagtcc gtgccggttg
      481 ggatcgatac cagcgaccag ccgccgcgcc tgaccaagga tccgtcgcgg ttgcgcctgc
      541 cagaggaaac ttcgccctcg agcacgaagc cggcttccgc gccgtcgtcg cctggcgcga
      601 gcagcgatga gcctccgcgc ttgtccaagc cttcagcgcc atcgtcacca ccctcctcac
      661 catcgccttc ttcggcacct tctgggggat cgaatccacc ggggtcgagt gcgcctgcgc
      721 cgggggcagc accggtaggg tcttcctcgc catctgcttc gcagtcgaaa gcaggcgata
      781 ccaaggcgga ggcaaaaact ccggaatccg acagtggcgc cagcgacgct aaccggccca
      841 ggctgcggcg cggcaagccc accgcgcctt tgcccgacga ggaggtagcg ggctacggca
      901 aacccggtgc ggagcccgct gccggcgcag caggggcatc cccggccccc gccaacgaag
      961 ggcagttcca gctgattccg gcgatctccg acgccaaagg gccagagccg cgtccgttta
     1021 ccttcgaatg gctaaaggac gaagaaggcg agcgccgtca gcagctgaca acgatggcca
     1081 aagatgagct tcgcgcctat ctcgacgcgc aagccaaggc gagaattgca cccatcgcga
     1141 ccacgacgcc gaagactacc cggcacacgc cgccggccaa gcctcctgag ccgatccttg
     1201 agaacgtgcg catgaacgcc tacgacctat gggtgagcaa tcagcccgtc atcgtgttca
     1261 gcgcggaagc gcatgtgcca gccacaccca cgcctagcgg gcaggccacg ccggaatcaa
     1321 acctgcagta ctcgatcctg ctggtgactt atcctgacat ttacaacaac ttgcgcaagc
     1381 tctatgttgg ggtgaccgac aagtatcacc tcgacatcac tcccaagctg cagttggtgg
     1441 atgccgtgga cgtcgacggc gacggccgcg gcgaactgct cttccgggag acctccgatg
     1501 ctgggggagg ctgggtgatt tatcgtgcaa gcgccgacaa actgtggaag atgtttgaca
     1561 gtttgaatcc ggagtagcag ctgcccgtct ccacaacccc gactttttcg ctaaaatgtc
     1621 ccggcgtctt tgaaaggggc aaggattcta tgcgcaagac ttcacttctc ttagctctgt
     1681 ttctcgtttc cggcttggcc gccgcccagg accaggattt cagcaaagta cagatgaagg
     1741 tgacgaaagt cgccggcagc gtttacatgc tggaaggcgc gggcggaaat atcggggcat
     1801 cggtgggaga cgacgggatc gtggttgtcg atgatcaata cgctccgctg gccgagaaga
     1861 tccaggctgc gctgaagggc ataactgaca agccggtgcg cttcgtcatc aatacccact
     1921 atcacgaaga ccacacgggc gggaacgcct atttccagaa gcaggcgccc atcatcgcgc
     1981 acgataacgt gcgcaagcgg ctggagagcg gcggcacggc gggcgtcgga ccgggtggat
     2041 cgatgcattt cgaggccaag ccgcaaccca aggaagcgct gcccatcatc actttcgacc
     2101 acgatgtgac cgtacatctc aacggcgagg acattcgcgc gctctacttc cctgccggcc
     2161 acaccgacgg cgattccgtg attttctttc ccaagtcgaa cgtcgtgcac atgggcgacg
     2221 acttcgtgac ctacggtttc ccgttcattg atgtcgacag cggaggcagc atcgacggca
     2281 tgatcgacgg cgtggagaag gtcatcgccc aggtgcctgc ggacgtgaag atcattcccg
     2341 ggcatgggcc gctttcgaat ctcgatgacg tacgggcgta cttgaagatg ctgaaggaaa
     2401 cgcgggccgc cgtcgccggc gcactggaga agcaccagac gctggcccag atgaagcagg
     2461 cgaagctgct cgatccatgg aagaagtact ccggcgactt catcaacgag gatgcgtttc
     2521 tggagacgct ctacaactcg ctgaccggac agccgaacgg gaagttcatc aagcacaact
     2581 agggccgccg ctgcgagcgg cccaagactt tcgaaggaga ttcaactctg tcttgcgact
     2641 gacacgccga aggtttgtcc aatcgattct gggggccggt gccacggctg gtctgccggc
     2701 tctgttttcg gcatgtaccg ggtcttcgag ggctggcacg tcggcctcgg gcgccgttgc
     2761 ggcgcggcag ttcagcggcg ctgtcgacgc cgtttttccc aggggatttt tctggggtgc
     2821 tgcgaccgct gcctatcaga tcgagggcgc ctggaacgag gacgggaagg gcgaatccat
     2881 ctgggaccgg ttcgcgcaca ctccgggcaa gatcaagaac ggcgacaccg gcgacgtggc
     2941 ttgcgattcg taccatcgct ggcgcgatga catcgcgctg atgcgggcga tgcatttgaa
     3001 tagttaccgc ttctcgattt cctggccgcg cattcagccc gctggatcgg gcgcggcgaa
     3061 tcgcaagggc gtcgactact acagccgcct cgtggatgcg gtgcttgagg cgcacattcg
     3121 cccgctggtt acgctctacc actgggatct gccgcaggcg ctcgaagaag ccggcggatg
     3181 gcccaatcgc gacaccgccg cccgcttcgc agactacgcg caactggtgg cgcaggcgct
     3241 gggagatcgc gtttccgact ggatcatttt caacgagccg gatgcttttg tggatcttgg
     3301 gtatctggaa ggcacgcacg cccccggcca caagagcctg ctcgactttc tgcgggctac
     3361 ccacgtggtc aatctggcgc agggagcggg gtttcatgcg cttaaagcag ttcggccgtc
     3421 tgcccgcgtg ggcacggctt tcagcatgtc gccctgcgag cctgcgacgg agtccgccga
     3481 ggacaaactc gcggcggagc gggcccatgc catcagcaac atctggtttc tggagccggc
     3541 gctgcgcggg cgctatccgg aggcactgac tttcctgccg gagacggcga tgggcgtgaa
     3601 atcgggcgat atggaaaaga cgcgcgcgcc gctcgatttc attggtgtca atctttacta
     3661 ccgcacgatc gcgtcggcgc caggcacgat ggaacgcttc gcccatgctc aggattggct
     3721 gtttcccgtg aagatggacg gagggc
//
LOCUS       DASPUL010000107         4874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1797045, whole genome shotgun sequence.
ACCESSION   DASPUL010000107 DASPUL010000000
VERSION     DASPUL010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1797045"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VEJ00_02500"
     CDS             <1..265
                     /locus_tag="VEJ00_02500"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXX00057.1"
                     /translation="TEARGERIRSRTRELTVLHQGQSLGMITVSVGVAVLPEHGTNPK
                     ELLEAADAALYRAKREGRDRVVVAEVAAGADALVASLVESKKS"
     gene            complement(278..1060)
                     /locus_tag="VEJ00_02505"
     CDS             complement(278..1060)
                     /locus_tag="VEJ00_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXX00058.1"
                     /translation="MPPDQTQEKNMATAEVISVPTPLENAPPDSCMIAAEDLWKTYDM
                     GSEQQVHALRGVNLRIRRNEYVAIMGPSGSGKSTLMNLIGCLDSPTKGRYWLNSQLVS
                     ELDDDELAHIRNKEIGFVFQTFNLLARATALHNVELPLIYAGIPTEERLERARASLTA
                     VGMEERMSHKPNELSGGQRQRVAIARALVNKPSIILADEPTGNLDSQTGLEIMALFDR
                     LHAEGNTIILVTHEHDIAEYAHRVIHIKDGVIASDESKSQQN"
     gene            complement(1211..2602)
                     /locus_tag="VEJ00_02510"
     CDS             complement(1211..2602)
                     /locus_tag="VEJ00_02510"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXX00059.1"
                     /translation="MKLWKKIAIGAGIALVLALVVGFTVHQSSKNVVTVQTGKVQRQD
                     LATIVSASGEIKPKTYVNIGANAFGKITHLYVKEGDRVKKGQMLAQLENVQSSADVHA
                     GEASLQAAQTDAIAADAALKTAQADLLRAQADYDRNKLDWNRAQNLYKDGLISKSDFD
                     SRQNAWATADAGLTQARARVAQAQAQKESADRHVSQNQANLTRVNDVLQKTSYAAPFD
                     GVVTNLPVREGESVVIGIQNAMGSTLMTIADMSVITAEVKVDETDIVNVRLGQPADVT
                     IDAIPNKTFHGNVSEIGDNAIVRSSGVSTSQQATASEEAKDFKVVVYLTDAPPDLRPG
                     LSTTAKITTATRSNALSLPIQALTLRTKEQIEQQNNPKGSVHAATPAASDPASKAKKG
                     DVQGVFVIRNKKAMFVPVTTGIIGTTDIEVLDGVKDGDEVITGSYKVLRTLRPGSSVK
                     IDNSAPKKEEETS"
     gene            3021..4856
                     /locus_tag="VEJ00_02515"
     CDS             3021..4856
                     /locus_tag="VEJ00_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HXX00060.1"
                     /translation="MFPVTSFQTFLRVALVSAGLLISGAVAQGQAQGQAKDQTKDQAT
                     TQAQSQPDQNSPDQAVTDKPAPDSASSTKAQPAVDAADPMKRPIDAKKKKQQMKDYSK
                     EIKGPYKKWLDEDVVWIITDEEKAAFKQLSNDEERDNFIEAFWQRRDPTPDTEENEYK
                     EEHYRRIAYANEHFAAGIPGWKTDRGRIYIMYGPPDEIDSHPSGGTYERPIEEGGGET
                     STFPFEDWRYRYIEGIGQEIIIEFVDDCQCGAYEMTMDRSKKDALLYTPNAGLTLYEQ
                     MGMASKTQRFTGGGLERLGAGPMASDLQTKEFDRLEQYAKLQAPPPVKFTDLLETVTS
                     KVILNPMPFDVRTDFVKVTSDTVLVPVTIQMKNRDITFVNKDGVQRGTVNILGRLTTL
                     TGKVAQTFEDTVQVDVPAELLPRTAENSSVYWKALPLRPGRYKLNVAIKDVNGDRKGL
                     WSRSIIVPEFAEDKLSTSSLIVADVMEPVATKDIGTGSFVIGTMKVRPRVPPADNSKP
                     AVFKKDRDQKLNFWMQVYNLGVDEKTHKPSATFEYDIVNVATNKPVVQKVESTETMGN
                     VGEQVTLQKSISSANLQPGVYKIEIKVNDNISKQTVDPSAMFAVE"
BASE COUNT          945 a         1408 c         1540 g          981 t
ORIGIN      
        1 tacggaggct cggggcgaac gaattcgctc ccgcacgcgc gagctgaccg tgttgcatca
       61 ggggcagtcg ctggggatga ttacggtctc ggtaggagtg gcggtgcttc ctgagcacgg
      121 caccaatccc aaggagctgc tggaagctgc ggacgcggcg ctttaccggg ccaaacggga
      181 aggccgggat cgcgtggtgg tagcggaggt tgcagcgggc gccgatgcgc tggtggcgag
      241 cctggtcgaa agcaagaagt cgtgacggcg tgagcgctca gttctgctgg ctcttgcttt
      301 cgtcgctggc gataacgcca tccttgatgt gaatcacgcg atgagcgtat tcggcgatgt
      361 cgtgctcgtg cgtgaccagg ataatggtgt tgccttcggc gtgcaggcgg tcgaacagcg
      421 ccatgatctc caagccagtt tgcgaatcga ggttgcccgt gggctcgtcc gccaggatga
      481 tggagggctt gttcaccaga gcgcgggcga tggcgacgcg ctggcgctgg ccgcccgaga
      541 gctcgttggg cttgtggctc atgcgctctt ccatgcctac tgcagtcagg gaagcgcggg
      601 cgcgctccag gcgttcttcg gttgggatgc cggcgtagat caagggcagc tccacattgt
      661 gcagggccgt ggcccgggcc agcaggttga aggtctggaa gacgaatccg atttctttgt
      721 tgcggatgtg cgccagttcg tcgtcgtcaa gctcgctgac cagctggctg ttgagccagt
      781 agcggccctt ggtcggagag tcgaggcagc cgatgaggtt catcaaagtc gacttgcccg
      841 aaccggacgg acccatgatg gcgacatact cgttgcgccg gatgcgcagg ttgacgccgc
      901 gcagggcgtg cacctgttgc tccgagccca tgtcgtaggt cttccagaga tcctcggccg
      961 cgatcatgca tgagtcgggc ggcgcattct caaggggtgt gggcacgctg atgacttcgg
     1021 cggttgccat gttcttctcc tgcgtttggt ccggtggcac tcagctctcc tcgttcaggg
     1081 gccaggggtc agaggtcagg gaccagcaaa ccctgcaccg cgcatctgac caccgaccac
     1141 tggtccctga cccctagccc ctgacctcta atccctggcc actgtttttg acgcgccacg
     1201 ctcgccaacg ttatgacgtt tcctcttcct tcttgggagc gctgttgtcg atcttgacgc
     1261 tgctgcccgg gcgcagcgtg cgcaagacct tgtaactgcc ggtgatgacc tcgtcgccgt
     1321 ctttcacgcc atcgagcact tcgatgtcgg tggtgccgat gatcccggtt gtgaccggca
     1381 cgaacatcgc ttttttgttg cggatgacaa agactccctg cacgtcgccc ttcttcgcct
     1441 tggatgcagg atcgctggcc gcgggtgtcg cagcgtgcac cgaccccttg ggattattct
     1501 gctgctcgat ctgttccttg gtgcgcaggg ttaaggcctg aatggggagc gagagcgcat
     1561 tgctgcgggt cgcggtagtg atcttggccg tggtggaaag tccggggcgc aggtccgggg
     1621 gcgcatcggt caggtagaca acgaccttga aatccttggc ttcttcgctg gcggtggctt
     1681 gctgggaagt cgagacgccg ctggagcgca cgatggcgtt gtcgccgatc tccgacacgt
     1741 tgccgtgaaa cgtcttgttg ggaatggcgt cgatggtgac atcggcaggc tggccgaggc
     1801 gcacattcac gatgtcggtc tcatcaacct tcacttcggc ggtgatgacc gacatgtcgg
     1861 cgatcgtcat cagcgtgctg cccatggcat tctggattcc gatgaccacg gactcgcctt
     1921 cgcgcaccgg caggttggtc acgacgccgt cgaagggcgc cgcgtagctg gttttctgca
     1981 gtacgtcgtt gacgcgggtg aggtttgcct gattctggct gacgtgccgg tcggcggatt
     2041 ccttctgagc ctgggcttgc gcgacgcgcg ctcgtgcctg ggtcaggccg gcatcggcgg
     2101 tggcccaggc gttctgccgg ctgtcgaagt ccgacttgga gatcaggccg tctttgtaca
     2161 ggttctgtgc ccgattccag tcgagtttgt tgcggtcata gtcggcctga gcgcgcagca
     2221 ggtcggcttg cgccgttttc agggcggcat cggccgcgat ggcgtcggtc tgcgccgcct
     2281 gcagcgacgc ttctccggcg tgcacatcgg ctgaagactg cacgttctcc agttgtgcca
     2341 gcatttgccc ctttttcacg cgatcaccct ctttcacgta caggtgggta atcttgccga
     2401 aggcgttggc gccgatgttg acgtaagtct tgggtttgat ttcacccgag gcgctcacga
     2461 tggtcgccaa atcctgccgc tgcaccttgc cggtttgtac cgtaacgaca ttcttgctgc
     2521 tctgatgaac ggtaaacccg acgacgagtg ccagcacgag cgcaatacct gcgccgatcg
     2581 cgattttctt ccagagtttc atgttgccgt cacagtctgg ggcgacgcct gcccttcaca
     2641 cctctaccat ggcaaccgct gcatgatggg ctgggccgga tttgtcttct gcaggttccg
     2701 cttgggtgac ggacacaacc acctcgtaca tcaggtagta cgaccctgcc gccgagaaag
     2761 ttcccaatcg ggaaggatga tggccgagac cctacgctgg aaattaccgc taccgccccg
     2821 gggacacttt attatagctt tccttcgctg gtccggcagc caccgaattg gcacaagctg
     2881 caggcaactc ggggcggggc cgggcgcgtc caagtaagac agccgttaat tggttcacgg
     2941 ggcgagactt aaccttgctt tcacacaacg aaacgaccta gaataggcac acgtacggtt
     3001 gtctggcagg agttcctctc atgttcccgg ttacttcgtt tcagactttc cttcgtgtgg
     3061 cgctggttag cgcggggttg ctgatatcgg gcgccgttgc ccagggccag gcccagggcc
     3121 aagccaaaga tcagaccaag gatcaagcca cgacccaagc tcagagccag ccggatcaga
     3181 actccccgga ccaagccgta accgacaagc ctgccccaga cagcgcgagt tcgacgaagg
     3241 cacagcccgc cgtcgatgcc gctgacccga tgaagcgccc catcgacgcc aaaaagaaaa
     3301 agcagcagat gaaggactac tccaaggaaa tcaaggggcc ttacaagaag tggctggacg
     3361 aggatgtggt ctggatcatc accgacgagg agaaagccgc cttcaaacag ctttcaaacg
     3421 acgaggagcg ggacaacttc atcgaagcat tttggcagcg ccgcgatcct acgccggata
     3481 cggaagagaa cgaatacaaa gaagaacact accggcgcat tgcctacgcc aacgagcatt
     3541 tcgccgccgg catcccggga tggaagaccg accggggcag gatctacatc atgtacggtc
     3601 cgccggatga gatcgattcg catccgtcgg gcggaaccta cgaacggccc atcgaggaag
     3661 gcggcggcga gacgtcgacg ttcccgtttg aagactggcg gtaccgctac atcgagggaa
     3721 ttggccagga gatcatcatc gagttcgtgg acgactgcca gtgcggcgcc tacgagatga
     3781 caatggaccg ctcgaagaaa gacgctctcc tctatacgcc gaatgccggc ttgaccttgt
     3841 atgagcagat gggcatggcg agcaaaacgc agcgctttac gggcggcggc ctggagcgac
     3901 tgggtgccgg gcccatggcc tccgatctgc aaaccaagga attcgaccgc ctggagcagt
     3961 atgccaagct gcaggcgccg cctccagtca aattcaccga cctgctggag accgtcacct
     4021 cgaaggtcat cctgaatccg atgccgtttg atgtgcgcac ggatttcgta aaggttacca
     4081 gcgacacagt gctggttccg gtgaccatcc agatgaagaa ccgcgacatc acgttcgtca
     4141 acaaggatgg cgtgcagcgc gggacggtaa acattctggg ccggctgacc accctgacgg
     4201 gcaaggtcgc gcagacgttc gaagatacgg tgcaggtgga cgtgcccgcc gaattgctgc
     4261 cgcggacggc cgagaactcg tcggtctact ggaaggcgct gccgctgcgg cccggccggt
     4321 acaagctgaa tgtcgcgatc aaggatgtga atggcgaccg caagggcctg tggagccgga
     4381 gcatcatcgt tccggaattc gcggaggaca agctctccac ctcgagcctg atcgtggcgg
     4441 atgtgatgga gccggtcgcg accaaggaca tcggcaccgg gagtttcgtg atcggtacca
     4501 tgaaggtgcg gccacgagtt cctcctgccg acaacagcaa gcctgcggta ttcaagaaag
     4561 acagggacca gaaactgaat ttctggatgc aggtgtacaa tctcggcgtg gacgagaaga
     4621 cgcacaagcc ttccgccacg ttcgaatacg acatcgtgaa cgtcgccacc aacaagccgg
     4681 tggtgcagaa agtcgaaagc acggaaacga tgggcaatgt aggcgagcag gtaacgctgc
     4741 agaaatcgat ttcgtcggcg aacctgcagc ccggcgtcta caagattgaa atcaaagtga
     4801 acgacaacat ctccaagcag acggttgatc cgtcggcgat gtttgccgta gagtaactcc
     4861 ctgcaaattc tccc
//
LOCUS       DASPUL010000108         3775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_975663, whole genome shotgun sequence.
ACCESSION   DASPUL010000108 DASPUL010000000
VERSION     DASPUL010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975663"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1330
                     /locus_tag="VEJ00_02520"
     CDS             308..1330
                     /locus_tag="VEJ00_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00061.1"
                     /translation="MAGKTFWWAIFMAILASTLALAASSGYRVVTTYKVGGDGGWDYL
                     TADSDARRLYISRATHVMVLDADSGKSVGDIPDTPGVHGIALAPELGRGFTSNGREGT
                     VSIFDIKTLAVSNKVKVGENPDAILYDPATKRVFTFNGRSQDSSAIDAASGKVLATIK
                     LDGKPEFAASDAKGEVFVNIEDKSELVAIDADKMEVKAKWPLAPCEEPSGLSIDRKNR
                     RLFVGCGNKMMAVVDADSGKVLATPPIGDGVDATTFDPETGLAFASCGEGVLTVVKED
                     SPGKFSAENVPTQQGARTLALDSKTHNVFVVTAKFGPPPAATTDNPHPRRSIMPDSFV
                     VLVVGK"
     gene            1357..2628
                     /locus_tag="VEJ00_02525"
     CDS             1357..2628
                     /locus_tag="VEJ00_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HXX00062.1"
                     /translation="MLRVAAAIYLVISLIEPSRAYSVLTHEQVVDLLWEDRIQPMVKE
                     HFPGLTDKQLREAHAYAYGGSLVQDMGYYPFGSRYFSDLTHYVRTGDFVTCLIQQART
                     ANEFSFALGALAHYAADNSGHPMVNHVVAMEFPNLQKKYGDIVTYADSPKAHIRAEFG
                     FDMTEVAKNHYTSDRYHDFIGFEISQGLLERVFPIVYGIKFDDVVDDEDLAIGSFRHA
                     VSKVIPEMTRVALLNRRKEIVANTPGVAEKKYLYYLSRTDYEQEWGSEYRRPGFWTKA
                     LAILLKLLPKKGPFSGLDFKIPSQQAEDLFVQSVDETVSSYERLLGDVERGALHLSNR
                     DCDTGRETQPGEYVLSDATYAHLLDDLSDSDFKQVSPELRQDILTFYGNPDAPLATKR
                     KKKDWKRVQDELAQLRNSSTELKSDRQPGAK"
     gene            complement(2669..3196)
                     /locus_tag="VEJ00_02530"
     CDS             complement(2669..3196)
                     /locus_tag="VEJ00_02530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX00063.1"
                     /translation="MDYSISPLLPQHWPAVRAIYAEGIATGNATFETDLPDWTKWDSG
                     HRKDSRLVAVAERSNNAESGAESQAVLGWAALSPVSGRCVYGGVAEVSVYVAAAARGR
                     GVGKALLQALVKESEAQGIWTLQAGIFIENVASIALHKSCGFREVGTRHRLGKLGTVW
                     RDVAQLERRSKVVGV"
     gene            complement(3296..3619)
                     /locus_tag="VEJ00_02535"
     CDS             complement(3296..3619)
                     /locus_tag="VEJ00_02535"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047869 - dimethylpropiothetin
                     dethiomethylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfonioproprionate lyase family
                     protein"
                     /protein_id="HXX00064.1"
                     /translation="MKALFARLAEIEGLRAQSGKFYREFLRVPAMSAGLYVLGPGATD
                     PQRPHHEDEMYYVVRGRGRFRAGDEDREISTGSVLFVAAEVEHRFHDITEELAILVFF
                     APAES"
BASE COUNT          799 a         1083 c         1156 g          737 t
ORIGIN      
        1 ttgatgattt ctttgcctgc gccgcgcctc ccatggtcag agcaaagaga cagagcaata
       61 ctgcaataag gcgcttcgtc attaggtttc cctcctagga atccggcgga attttcgcac
      121 aatgtgggag aaaccacaaa aagacgagaa cgtgggagga gcccgccgcc gacggctcca
      181 ggtgcagcct cgaaataggg taaagtactt ccggtcgaat ccggcccggc tattgccttc
      241 acgtagtcgt cggcctgaag aattcggaac gtagcgagtg ctgcaaccta accgcgagga
      301 ggaactcatg gccggaaaga ctttctggtg ggcgatcttt atggcaatac ttgcctcgac
      361 gctggctctg gctgccagtt cgggatatcg cgtggtcacg acttacaaag taggcggcga
      421 tgggggctgg gattatctca cggctgactc cgacgcgcgt cggctctaca tttcccgcgc
      481 cacccacgtg atggttctcg acgcggattc gggcaagagc gtgggcgata tccccgacac
      541 gcccggcgtg cacggcatcg cactggcccc cgagctcggg cgaggcttca ccagcaacgg
      601 tcgcgaaggg acggtcagca tttttgacat caaaacgctc gccgtgagca acaaagtgaa
      661 ggtcggcgag aaccccgatg cgattttgta tgacccggct acgaagcgag tcttcacttt
      721 caacgggcgc agccaggatt cttcggcgat tgacgccgcc agcggcaagg ttttggcaac
      781 gatcaaactc gacggaaagc cggagtttgc cgcgagcgac gccaaaggcg aagtcttcgt
      841 gaatatcgag gacaagagcg aactggtcgc catcgatgcc gacaagatgg aggtgaaagc
      901 aaagtggccg ctggcgccct gtgaagagcc gagcggactc tccatcgacc gcaagaatcg
      961 ccgcttgttt gtgggctgcg gcaacaagat gatggcagta gtcgacgccg acagcggcaa
     1021 agttctggcg acgccgccga tcggcgatgg cgtcgatgcg acgacattcg atcccgaaac
     1081 cgggttggcg tttgcctcct gcggcgaggg tgtacttacg gtggtgaagg aggactctcc
     1141 gggaaagttc agcgccgaga acgtcccgac ccagcagggc gcgcgcacgc tggcactcga
     1201 ttccaagacc cacaatgtgt ttgtggtgac ggcaaagttc ggaccgcccc cggcagctac
     1261 taccgacaat cctcatcccc ggcggtcgat tatgccggat agtttcgtgg ttctcgtggt
     1321 cggaaaatga gtcgatcttc tcatcgcgga gaagctttgc tccgtgtcgc ggcagcgatc
     1381 tatctggtca tatccttgat cgagccgagc agggcttatt ccgtattaac ccacgagcag
     1441 gtcgtcgacc tgttgtggga ggaccgcatt caacccatgg tgaaggaaca ctttccgggt
     1501 ttgacggata agcaactgcg cgaggctcac gcctatgcct acggcggatc gctggtgcag
     1561 gacatgggct actacccgtt cggcagccgc tattttagcg atctgacgca ctacgtgcgg
     1621 accggagact tcgtgacttg cctgattcag caggcgcgca cggccaatga attctcgttc
     1681 gcgctgggag cgctggccca ttacgcagcc gacaacagcg ggcatcccat ggtgaatcat
     1741 gtcgtggcca tggagtttcc gaacctgcag aagaagtatg gcgacatcgt gacctatgcg
     1801 gactcgccca aggcccacat ccgcgccgag ttcggctttg acatgaccga ggtggcgaag
     1861 aatcattaca cctcggaccg ctaccatgac ttcattggct tcgagatttc ccagggcttg
     1921 ctggagcggg tatttccgat cgtctacgga attaagttcg acgacgtggt cgacgatgag
     1981 gaccttgcca tcggaagttt tcgccacgcc gtcagcaagg tgattccgga gatgactcgc
     2041 gtcgccttgc tgaaccggcg aaaagagatt gttgccaata ctcccggcgt ggcggagaag
     2101 aagtaccttt actatttgtc gcgcacagac tacgagcagg aatggggcag cgagtaccgg
     2161 cgcccaggtt tttggacaaa ggcgctggca attctgctga agctgttacc gaagaaaggg
     2221 ccattcagcg ggctggattt caagattcca agccagcagg cggaggatct tttcgtgcag
     2281 agtgtcgacg agacggtcag cagctatgag cgactcttgg gggatgtgga gaggggggca
     2341 ctgcaccttt ccaatcggga ctgcgacacc ggaagggaaa cacagccagg agaatatgtg
     2401 ctgtccgacg cgacatatgc tcacctgctg gatgatctgt cggacagcga cttcaagcag
     2461 gtctcgcccg aactgcggca agatattctg acgttctacg gaaatccgga tgcgccactg
     2521 gcgaccaaac gaaagaagaa agactggaaa cgagttcagg atgaactggc gcaactgaga
     2581 aattcttcaa ctgagttgaa gtccgaccgc caacctggcg cgaaatagtc gtgcggggga
     2641 atcggcgagt ggcccgctcg accacgactt acactccgac aactttgctt cgacgttcga
     2701 gttgggctac atcgcgccat acggtgccga gtttcccgag gcggtgtctg gtcccgactt
     2761 cccggaaccc gcaggatttg tgcagcgcga tgctggcaac gttctcgatg aagatgcccg
     2821 cctgcagcgt ccagatgccc tgcgcttcgg actctttcac cagcgcctgc agcagtgcct
     2881 tgcccacgcc gcgtccgcgg gctgcggcgg ccacgtagac tgacacttcc gcgactcctc
     2941 cgtagacgca gcgccctgaa actgggctga gcgcggccca gcccagcacg gcttgcgatt
     3001 cggcgccgct ctctgcattg ttggacctct ctgcaactgc gaccagccgg gagtctttgc
     3061 gatgtccgct atcccatttc gtccagtcgg gaaggtcggt ttcgaacgtg gcattgccgg
     3121 tcgcaatgcc ctcggcgtag atcgcccgaa cggcgggcca gtgctggggc aataacgggg
     3181 aaatcgaata gtccataggt aagattgccc tcagcggcta aagccacagt tgagtgtgat
     3241 tcctggcggc gcggctgaaa gccgcaccct taaaaagcgc agcgctctcg acgatctagc
     3301 tttctgcagg ggcgaagaac accagtattg ccagctcctc tgtaatgtca tggaagcggt
     3361 gctccacttc ggcggcgacg aagagcacgc tgccggtgga gatctcacga tcttcatcac
     3421 cagcgcgaaa gcggccccgt ccgcgcacca cgtagtacat ctcgtcttca tggtgcgggc
     3481 gctgggggtc ggtggctccc gggccgagca cgtagagacc cgcactcatg gccggcactc
     3541 gaagaaactc tcggtagaac ttgccactct gggcgcgaag gccctcaatc tcagcgaggc
     3601 gggcgaagag cgctttcaca ggagcatgcc tccgaagacg agatcggtga actgcggcac
     3661 caggttctgc gcctgcaact cgccttccgg cttgtaccac tggtagatcc agttgatcat
     3721 gccgagcagg gcgaaggcgg cggcgcgcgg gctgaccgcg cccctggaat tgcgt
//
LOCUS       DASPUL010000109         4154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_18991, whole genome shotgun sequence.
ACCESSION   DASPUL010000109 DASPUL010000000
VERSION     DASPUL010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18991"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /locus_tag="VEJ00_02540"
     CDS             <1..1409
                     /locus_tag="VEJ00_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HXX00065.1"
                     /translation="ASKSYPASPSPIFDASVAAPDGLVITHEDIDRAHDRDRVWETRA
                     RRGLWSRARLLWLLVGPGILVMLGENDGPSMLSYAATGARFGIGFFLPFVVLTFLMAC
                     VVQEMTVRLGAATHRGHAELIFDRFGPFWGWFSMIDLMVGNFLTLITEFIAIRAGLGF
                     FGIPPVVSVSVAWLILFAALMAHRYWTWERVTLTAAMCNLIFVPVALLSHPHWGAIRS
                     SLFTWKPLPGGLTQETVLILLADIGATVTPWMLFFQQSAIADKGLTTRDIRFGRVDTV
                     LGAALASLAAVATIVATAPLFAHGMSTGNFEAAEFAQALRPFVGHWGAALFALGMFEA
                     GMVAAVTISTSSAYAFGEVARRPHSLNLPFRQGKSFYLVLILEAAAAAGLVLIPGIPL
                     VYIVLVVNVIAVLAMPPALLFLYMLVNDREIMGDLVSPPWANIMALGVVVLLIAAGVL
                     FGVSVAAPHALAFFGGNR"
     gene            1547..1765
                     /locus_tag="VEJ00_02545"
     CDS             1547..1765
                     /locus_tag="VEJ00_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00066.1"
                     /translation="MEHPTTNSEITSEALPSVVASLEPDQLISAKEKHHCPRRQLTGT
                     EKFLFWALRIYLVFMFGVVLYQVWTGAR"
     gene            complement(1923..2141)
                     /locus_tag="VEJ00_02550"
     CDS             complement(1923..2141)
                     /locus_tag="VEJ00_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00067.1"
                     /translation="MKKLRVIVLLALLGASAFAPIHAYARNNANSQARKAQKKQQKAM
                     KKYLKAQKKAQKKMIKQDRKNTHLPSHY"
     gene            2489..>4154
                     /gene="acnA"
                     /locus_tag="VEJ00_02555"
     CDS             2489..>4154
                     /gene="acnA"
                     /locus_tag="VEJ00_02555"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HXX00068.1"
                     /translation="MSVNSFNSRSSLKVGSKEYEIHRLDALDKQGISTKHLPFCLRIL
                     LENLLRTEDGRNVTKEEIRALASWNKNSKPDKEIAFTPSRVLLQDFTGVPCVVDLAAM
                     RDAMKQLGGDPALINPLQPVELVIDHSVQVDEFGTSNAFEMNALLEFQRNKERYSFLR
                     WGQTGFRNLAIVPPDTGIVHQVNLEYLARVVFVQETDGSRSAKSPAYPDTLVGTDSHT
                     TMVNGLGVLGWGVGGIEAEAAMLGQPVSMLIPLVVGVKLTGRLREGATATDLVLTITE
                     MLRKHGVVGKFVEYFGPGLRDLPLADRATIANMSPEYGATCGIFPIDKETLRYLKLTG
                     RSAAQIALVEAYSREQGLFHDEKTPEAEYSELLTLDLGTVEPSLAGPKRPQDRVVLSQ
                     AGESFSKALPSLIKPKSAAKTAPAATNGPRWEQEGGNPAAIGVEDPNDHEHVSASVKD
                     SLKHGSVVIAAITSCTNTSNPSVLIGAGLLAKKAVEKGLTVPAWVKTSLAPGSKTVTD
                     YLAKAGLTPYLEKLKFHLVGYGCTTCIGNSGPLPEEVSKAIDEKELV"
BASE COUNT          758 a         1417 c         1152 g          827 t
ORIGIN      
        1 cagcctcgaa atcgtacccg gcgtcgcctt cgcccatctt cgatgcctct gttgccgctc
       61 ccgacggcct cgtcatcacc cacgaagaca tcgaccgcgc ccacgatcgc gaccgggtct
      121 gggaaacccg cgcccgtcgc ggcctctggt cccgcgcccg cttgctctgg ctgctcgtcg
      181 gaccgggcat cctcgtcatg ctcggcgaaa atgacggccc cagcatgctc tcttacgccg
      241 ccactggcgc gcggttcgga attggttttt ttctcccctt cgtcgtcctc accttcctca
      301 tggcctgcgt cgtgcaggaa atgaccgtcc gccttggcgc cgccacccat cgcggccatg
      361 ccgaactcat cttcgaccgc ttcgggccgt tctggggatg gttctccatg atcgacctca
      421 tggtcggcaa cttcctcacg ctcatcaccg aattcatcgc tatccgcgca ggtttagggt
      481 tcttcggcat tcctccggtg gtctcagtgt cagtcgcgtg gctcatcctg ttcgccgccc
      541 tgatggctca ccgctactgg acctgggagc gcgtcaccct caccgccgcc atgtgcaacc
      601 tcatcttcgt cccggtggca ttgttgagcc atccgcactg gggcgcgatc aggagttcgc
      661 tgttcacctg gaaaccgctc cccggcggcc tgacccagga aacggtcctg atcctgctcg
      721 ccgacatcgg cgccaccgtc acgccctgga tgctcttctt ccagcaaagc gccatcgccg
      781 acaagggcct gaccacccgt gacatccgct tcggccgcgt cgacaccgtg ttaggcgcgg
      841 ctctggcgtc gctggccgcc gtggccacca tcgttgccac cgcgccactt ttcgctcacg
      901 gcatgtcgac gggcaacttc gaagcggccg agttcgccca ggccctgcga cctttcgtcg
      961 gccactgggg agccgcactt ttcgctctgg gaatgtttga agccggtatg gtcgccgccg
     1021 tcaccatctc aacctcctct gcctatgcgt tcggagaagt cgcccgccgc cctcacagcc
     1081 tgaatctccc cttccgccag gggaaatcgt tttatctggt gctgatcctc gaagccgccg
     1141 cggcggcggg cctggtcctg atccccggca tcccgcttgt ctacatcgtt ctcgtagtca
     1201 acgtgatcgc agtccttgcc atgccgcccg cgttgctttt tctgtacatg ctggtcaacg
     1261 accgcgaaat catgggcgat ctcgtcagcc cgccctgggc aaacatcatg gccctgggcg
     1321 tcgtcgtgct tctgatcgca gcgggcgtgc tgttcggcgt gagcgtggcc gctccccacg
     1381 ctctcgcatt cttcggaggc aaccgatgat ggcgttggtt tcccctgagc agttccagtt
     1441 caagggctac gtttgcaagc acggctttgg cggcgtcatc ccgaagcccc gcgctttcac
     1501 cagcaccggc cgtaagagca ccaccatgaa cttgaggcga ttctgaatgg aacaccccac
     1561 caccaactcc gagatcactt ccgaagccct gccctccgtc gtggcctccc tcgaacccga
     1621 ccagctcatc tccgccaagg agaaacacca ctgcccccgt cgccagctca cgggcacgga
     1681 aaaatttctc ttctgggctc tgcgaatcta tctggtgttc atgttcggag tcgtgctcta
     1741 tcaggtctgg acgggagcac gttagaaaca tcgcgcgctg caagaattgt ttgggaaggc
     1801 cgcgccttcc aggggcgccg acaggccgga actggtttga aagggcatgg ctttcagcca
     1861 cgccgttgaa gctagaagaa caggggcttc agcccctgag ggagcgcacg agagattgga
     1921 gcctagtaat gcgacggcag gtgagtattc ttccggtcct gcttgatcat cttcttttgc
     1981 gcctttttct gcgccttcaa atacttcttc atcgccttct gctgcttctt ctgtgccttc
     2041 ctcgcctgag agttggcgtt gttgcgggcg taggcatgga tcggcgcgaa ggcgctggcg
     2101 cccaacagtg caagcagaac gatgactcga agtttcttca tagattccta gcggtcgcga
     2161 gcactgcgga agggttctga tgctgcgacc ctgcaacatt catttaatca tacaggcggg
     2221 ccatcgcatc agtaccaaag tacttgcaag caaccgcttt tcgcatcccg aaaacgcatt
     2281 tcgacgcgac cgagcaccga cctcctctgc gtccttcgcg aaccctcagc gcactttgcg
     2341 gtaaagcact ccttggcccg tcctggaacg caacttcccc ccgaattgac cctgcgtccg
     2401 agcgcaattc cccacaaaac gcattacact agtaagttgc accccagcgc ggcgatccgc
     2461 ctgcgccccc ttactaagga aggccatcat gtccgtcaat agcttcaact cccgctcctc
     2521 cctcaaagtc ggcagcaaag aatacgaaat ccaccgcctc gacgccctcg acaagcaggg
     2581 catctcgacg aagcacctgc cattctgcct gcgcattctg ctcgagaatc tcctccgcac
     2641 cgaagacggc cgcaacgtca ccaaagagga aatccgcgcc ctcgccagct ggaacaagaa
     2701 ctccaagccc gacaaggaaa tcgccttcac ccccagccgc gtcctgctgc aggatttcac
     2761 cggcgtcccc tgcgtcgtcg atctcgccgc catgcgcgac gccatgaagc agctgggcgg
     2821 cgatcccgcg ctgatcaacc cgctgcagcc ggtcgagctg gtcatcgacc actccgttca
     2881 ggtcgacgag ttcggcacct ccaacgcctt cgagatgaac gccctgctcg aattccagcg
     2941 caacaaggag cgttactcgt tcttgcgctg gggccagacc ggtttccgca acctcgccat
     3001 cgtcccgccc gacaccggca tcgtccacca ggtcaatctc gaatacctcg cccgcgtcgt
     3061 cttcgtgcag gagacagacg gaagcagaag cgccaagagc cccgcctacc ccgacaccct
     3121 cgtcggtacc gacagtcata cgacgatggt gaacggtctt ggcgttcttg gttggggagt
     3181 gggcgggatt gaagccgagg ctgcgatgct ggggcaaccc gtgtccatgc tgattccgct
     3241 ggtcgtcggc gtgaagctga ccggacgcct gcgcgaaggc gcgaccgcga ctgaccttgt
     3301 gctgaccatc actgaaatgc tgcgcaagca cggcgtcgtc ggaaagttcg tcgagtactt
     3361 tgggcctggg ctgcgcgatc tgccgctggc cgaccgcgcc accatcgcca acatgagccc
     3421 ggaatatggc gcaacttgcg gcatttttcc catcgacaag gagacgctgc gctatctcaa
     3481 gctcacgggg cgcagtgccg cgcagattgc gctggtcgag gcctacagcc gcgagcaggg
     3541 acttttccac gacgagaaga cgcccgaggc ggaatactcc gaactgctca cgctcgatct
     3601 cggcacggtc gaaccgtcgc tggccgggcc caagcggccg caggatcgcg tcgtgctgtc
     3661 gcaggccggc gagtcgttca gcaaggcttt gccgtctctg atcaagccga agtctgcggc
     3721 caaaaccgcg cctgcggcga cgaatggtcc gcgctgggag caggaaggcg ggaatcctgc
     3781 cgccatcggc gtggaggatc cgaacgatca cgagcatgtt tccgcatcgg tgaaagactc
     3841 gctcaagcac ggcagcgtcg tcatcgcggc aatcacatcc tgcaccaaca cttcgaatcc
     3901 ttcggtgctg atcggcgcgg gactgctggc caagaaagcg gtcgagaaag gactgaccgt
     3961 tccggcatgg gtcaagacct cgctcgcgcc cggatcgaag accgtgaccg actatctcgc
     4021 caaggccggg ctgacgccgt atctcgaaaa actgaagttc catctcgtgg gctatggctg
     4081 cacgacctgc atcggcaact cgggaccgct gccggaagaa gtctcgaagg cgatcgacga
     4141 aaaagaactt gtgg
//
LOCUS       DASPUL010000110         3080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1661682, whole genome shotgun sequence.
ACCESSION   DASPUL010000110 DASPUL010000000
VERSION     DASPUL010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661682"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VEJ00_02560"
     CDS             complement(<1..182)
                     /locus_tag="VEJ00_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00069.1"
                     /translation="MPEVKTVTETTSATQSAPVPATTVKKSPGLTFRRLFTKPGVSPY
                     AEVDWELRTAQITDAHG"
     gene            complement(433..1242)
                     /locus_tag="VEJ00_02565"
     CDS             complement(433..1242)
                     /locus_tag="VEJ00_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00070.1"
                     /translation="MEAITRLVSESLARHGFDRPLDYRRLIWSRWFRCESTRSLLFVP
                     SKPGVFALAEEIIPTSSDVGADALVRPAERSSGNAPELGFSTSTSPAVEELRFSAAYR
                     PSIENAASAAEGRRMLAVTQFFEDDDMAYVLDRMLTRQHSRLASGRYFVRFVVIEDQL
                     QRRSICNALNQWIANSAERASGIGSHFASSLELAPATEFAINGQTSLFENSDAIGNAH
                     VGTAALGCPAERSSAASTGLRTVSTIATAPQPDSGAAANIQRPSSPFPAGF"
     gene            2442..2912
                     /locus_tag="VEJ00_02570"
     CDS             2442..2912
                     /locus_tag="VEJ00_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00071.1"
                     /translation="MFHRRILPVCLFLAASAALASARDLALVSNKTNSVTVITVPDLV
                     KICKAQTNRWPDGKPVTFIMRAPATPEMKVLLEKVYELPEAQVKDLIVNANHGRANHP
                     AILVVDSDEDLVNKVASIPGALGVVDVYAINSSVAVIKVAGKLPLEPGYLLHGN"
BASE COUNT          566 a          846 c         1042 g          626 t
ORIGIN      
        1 ccgtgggcat cggtgatctg ggcggtgcgg agctcccagt cgacttcggc gtaaggagag
       61 acgccgggct tggtgaagag gcggcgaaag gtgaggccag gggacttctt caccgtcgtg
      121 gcggggacgg gggcagactg agtggcggag gtggtttcgg ttacggtctt aacttcgggc
      181 atcaggctct ctccatatta gattctatgc gtgttgcgcc cgattcggcg ccgggcagag
      241 ggcggagaat cgggaatttc gtttgttttg taaaagatcg atctgttggc tcgggtgact
      301 tcaggcgtcc ctacgggacg cggatgtttc tcgcacgcct acccgccact gacgtggcgg
      361 gctattgtcg atcgtcccct tcgactgcgc tcagggcagg ctctaaggga cgagctgtgg
      421 cttcccatgg gcttaaaagc ctgcggggaa gggcgaggag ggacgttgaa tgttggcggc
      481 ggctccggaa tcgggctgcg gggccgttgc aatggtgctc acggttcgga ggccggttga
      541 agctgccgag ctgcgctcgg ccggacagcc gagggcggct gtccctacat gggcattgcc
      601 gatcgcgtcg ctgttctcga acagagaagt ctggccattg atggcgaatt cggtcgctgg
      661 cgccagttcc agcgaactgg cgaagtgcga accgatgccg ctggcccgtt cggcggaatt
      721 cgcgatccac tggttgagcg cgttgcagat ggagcggcgc tggagctgat cttcgatcac
      781 gacgaagcgg acgaaatagc ggcccgaggc caggcgcgag tgctgccggg tcagcatgcg
      841 gtcgagtacg taggccatgt cgtcgtcctc gaagaattgt gtgacggcga gcatacggcg
      901 tccctcagcg gctgaagccg cgttttcaat ggacggacgg tacgcggcgc tgaagcgcag
      961 ctcttccacg gcgggcgacg tgctcgtgga gaagcctagc tctggtgcat tgcccgagct
     1021 gcgctcggcc gggcggacga gcgcgtccgc ccctacatca gaagacgtag ggatgatttc
     1081 ctcggcgagg gcgaacacgc cgggtttgct ggggacgaac aggaggctgc gggttgattc
     1141 gcagcggaac cagcgcgacc agatcaggcg gcgatagtcg agcgggcgat cgaagccgtg
     1201 gcgcgccagc gattcggaaa cgagacgggt aatggcttcc attgcgggct cctaaagtac
     1261 tgtgctgcgt aattcattcg gcccacgccg acgacaagaa gtaccctcat ctgccgggcg
     1321 ggcttatttc catgtaactg tgctgctctg ctttcgtcgt cccctgggga ggaccggggc
     1381 cggccaccgc actttttccg gcttcgggct tcctgtgccg gaatgcctga gagccggttc
     1441 tgccttaggg ttgcgggcgc ccggtcacgg gtccaggagc cgaggcgccc aactgtttgt
     1501 tttgcctgcg gcgcagcacc gagtcacctc tggctcagag ccagcaccgc ggccgattgg
     1561 ttagttccct ttctctctca ctctttgtct cgccccattc ccgtgaggcg agattttgat
     1621 gccatgggat tgcaaagtta ctcccgtgga ttgtacctgt caagagggaa acaccatatg
     1681 ttgtatctcc agcaatgacg ggctagatgc ggacctgcgg acctgttttc cacacggagg
     1741 tcgcaagtcc gagtccgagt tcgagttcga gacggcgcag tttcacgaaa agaagactct
     1801 gggagtgtga gcgaagtcag acagcggcac aacgaaaaac gggctgaggt ctgttgatcg
     1861 gttgtggcaa agcggaaatt cctgtgcatg gctgtgggaa gcgggggatg ggagtgtacg
     1921 aatctcgtgg ttcggcgtga aagagcggag gtagcgccgc gggagggcgt ttctgagaat
     1981 tgcgccttag ccctggtgga cgattttgag tccatcgaac aacgctaccg gtcctacacg
     2041 gcgtcaatcg gtcgatcctg atgcggctct gaagcgcggc tcttccacgg ccgtgtcggc
     2101 ggcatcgcgt ttgaggaatg ggctcgcgcc tactcgctcc cccgattctt ggatgagtac
     2161 acgagcctaa tggtcggagt ggaagagcgc tcctttagcg ccgctcttcc acggagttcc
     2221 cggcaggcat ccgcgcgccc ggcgtttgaa agcagtcctc gcagaccgtg gcttcctgtg
     2281 atagggcgaa gtacaaaagc gcatgatcgg ggcgaggacg gccgctctcg atcgcggtga
     2341 aggatgttag ctccgaaggt acatgacctt cgtgaccgtt gagtggccgt tgaaattgca
     2401 agagaatcag cgcagggcga ctgtgccctg ctggagaggg cgtgttccat cgtcgaatct
     2461 tacctgtgtg tctgtttctt gcggccagcg ctgcgctggc ctccgcgcgc gatctcgcgc
     2521 tggtgtcgaa caagaccaac agcgtcacgg tcattactgt gcccgatctg gtaaagatct
     2581 gcaaagccca gaccaaccgc tggcccgatg gcaagccggt gacattcatc atgcgggcgc
     2641 ctgcgacacc ggagatgaag gtgcttctgg aaaaagtcta tgagttgccg gaagcgcagg
     2701 taaaagacct gatcgtcaat gccaaccacg gccgcgcgaa ccatcctgcg atcctcgtcg
     2761 tcgattccga tgaagacctg gtcaacaagg tggcgtcgat tccgggcgcg ctcggcgtgg
     2821 tggatgtata cgccatcaac agcagcgtcg cggtgatcaa agtggcgggc aagctgccgc
     2881 tggagccggg ctacctgttg catggcaact agccaatgcg gtgcgccgcg ggcgccgtcg
     2941 atctcttcgg tgacatggat cggtatggag acggagaaga aacccgagac gacttctgac
     3001 ctcaggccgt cacccggcaa atcaggaggc aaagtcacct tcagccggcg gtttccgcgc
     3061 ttcgtgctcg atgtgcggct
//
LOCUS       DASPUL010000111         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1116302, whole genome shotgun sequence.
ACCESSION   DASPUL010000111 DASPUL010000000
VERSION     DASPUL010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116302"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VEJ00_02575"
     CDS             complement(<1..367)
                     /locus_tag="VEJ00_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00072.1"
                     /translation="MKPYKTAALVIVMLVALASPNQSAAQHSRYRLVDLGALGGSGSF
                     SAWGSGPSSRGLVNQYGTTVGWADTSASDPFAAFCFYDCMVEHAFRSRHGGPLTDLGT
                     LSANTSSAANWISSNGLIAG"
     gene            590..2869
                     /locus_tag="VEJ00_02580"
     CDS             590..2869
                     /locus_tag="VEJ00_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXX00073.1"
                     /translation="MALDSRDSVSTSAILRFGTFEVDVRERELRKQGKLIRLQDQPFQ
                     VLFILLQRPGEVVGRDELRRQIWPQNTFVDFDNSLSAAVNKLREALGDSADRPRFIET
                     LPRRGYRFIAPVGADEPSKSRVSAASWQIPLFVSVVAVAAAGLAVAVLWRSREPRRLT
                     EKDTIVLGEFANTTNDPVFDGTLREGLSVQIAQSPFLSLASEDGIHQTLRLMGKAVDT
                     RLTPEVAREVCQRMNSVIALDGSIAQIGSRYDLILRAVDCANGDSLAGAEAQANDKSR
                     VLDALQSIAAEMRKKLGESSVTIQKYNVRLEQVTTPSLEALQAYSLGFQSLEDGNASA
                     AVGSLKRAVSLDPNFAIAYAALGTAYGPLGETSLAIENTRKAYDLRDRVSEREKFYIS
                     SHYEENVTGNTEKAGQVYELWAQTYPRDVVPVARLGFYYAALGQYDKSLAQGRRALEL
                     APDRGVAYEILAAGYLNLDRLDESETVIQQAKARGIDTPPLHEDGYALAFLKGDLAGM
                     ARETAWAAGRPGIEDRFIFVASETLACAGQLTQANAMTVRAAAMAREAGENETAADYL
                     AEAALREALVGNRAEARRRASAAWQASNGEDTGAALALALAGDRARPRKFADDLARRP
                     PQDAVDASTIRAAIALEEKDPARAIADLQGASPFELGDANELTLYPVYVRGQAYLAAH
                     HGAAAAAEFQKILDHPGLALNEIIFPLARLGLGRARVLAGDTPSARKAYEEFFTLWQH
                     ADPNLPVLLQAKAELARLH"
BASE COUNT          598 a          843 c          885 g          548 t
ORIGIN      
        1 ctccagcgat gaggccgttc gagctgatcc aattcgcggc gctgctggtg ttagccgata
       61 gcgtccccaa gtcagtcaaa ggaccgccgt ggcgcgaacg gaaggcgtgt tcgaccatgc
      121 agtcatagaa gcaaaacgcc gcgaaggggt ctgaagccga tgtgtcagcc cagccgacgg
      181 tggtgccgta ttgattaacg aggcctcggc tggaaggacc gcttccccac gcggaaaagc
      241 tcccggaacc tccaagtgcc cccaaatcga cgagtctgta ccggctgtgt tgtgcggcag
      301 attgatttgg gctcgccagc gcaaccagca tgacgatgac caatgcggca gttttgtatg
      361 gcttcatcgc gtcctttcaa tttcgaggcc ggttcgggcc ttgcgttgga gggggagcat
      421 ggatcacgct tgggggaata acaatggatt gcggcttgtt tggcggctaa ttggttctga
      481 accagcaaca cgttatttct gaatgaatta gggcgttgtc ttatctccag tttcaggctg
      541 cacccggaat ggtagacttc ccccggtaat cccaggggtg ggatgtaaca tggccctgga
      601 cagccgagac tccgtcagta cctccgcaat cctccgcttt ggaacctttg aagtggacgt
      661 gcgcgaacgc gaactgcgca agcaaggcaa gctcatcagg ctgcaggacc agccgtttca
      721 ggtcctgttc attctgctgc aacggcccgg cgaagtggta ggccgcgacg aactccgcag
      781 acagatctgg ccacaaaaca cattcgtcga tttcgacaac agcttaagtg cggcggtgaa
      841 caagctgcgc gaggcactgg gcgattccgc cgacaggccg cgcttcatcg agaccctgcc
      901 gcggcgcggc tatcgcttca tagccccggt cggcgccgac gaaccgtcga aatcgcgggt
      961 cagtgcagcg agctggcaga ttcctctttt cgtgtcggtg gtcgccgtgg cggcagccgg
     1021 gcttgcggtt gcggtgctgt ggcgctcacg ggagccacgc cggttgacgg agaaagacac
     1081 catcgtgctc ggtgaattcg cgaatacgac caacgacccc gtcttcgacg gaactctgcg
     1141 cgaaggactt tctgtgcaga tcgcacagtc gcccttcctg agtctggcgt cagaggatgg
     1201 aattcatcag actttgcgct tgatgggaaa ggcggtagat acccggctca ctcccgaggt
     1261 cgcgcgagaa gtctgccaga gaatgaacag cgtcattgcg cttgacggat cgattgcgca
     1321 gatcggtagc cgttacgatc tgattctcag ggcggtggac tgtgccaatg gggattcgct
     1381 ggccggcgcg gaagcgcagg cgaacgataa gagccgtgtt ctggacgcgc ttcaaagcat
     1441 agccgccgaa atgcggaaga agcttgggga atcctcggtc accattcaga aatacaatgt
     1501 acggctggag caggtgacga cgccgtctct cgaagccttg caggcgtata gtctcggctt
     1561 ccagtccctg gaagacggca acgcctccgc cgccgtcggc tcgttgaagc gcgcagtatc
     1621 tcttgatccg aacttcgcga tagcctatgc cgcgctcggc accgcctacg gccctctggg
     1681 cgaaaccagc ctggcgatcg aaaatacaag aaaggcctat gatctgcggg accgagtcag
     1741 cgagcgggag aagttctaca tttcatctca ctacgaagag aacgtaaccg gcaacacaga
     1801 aaaagcgggt caggtttatg aactctgggc ccagacctac ccccgggacg tggttccggt
     1861 tgcacgattg ggcttttact acgccgctct tggccagtac gacaagtcgc tggcgcaggg
     1921 tcgccgggca cttgaattag ctccagaccg cggagtagcc tatgaaatcc tcgccgccgg
     1981 atatttgaac ctcgatcgtc tggatgagtc ggaaaccgtc attcagcaag ccaaagcacg
     2041 tggcatcgat accccacctc tgcacgaaga cggctacgcc ctggctttcc tcaaaggtga
     2101 tctggcggga atggcgcgtg aaaccgcctg ggcggctgga aggccgggaa tcgaagaccg
     2161 gtttattttc gtcgcatccg agaccttagc ctgtgccggc caactcactc aggcaaacgc
     2221 tatgactgtg cgcgcggccg caatggccag ggaggcagga gaaaacgaga cggcggcaga
     2281 ctatctggca gaggctgcgc tgcgcgaggc gctggtcggg aaccgcgccg aagcccggcg
     2341 gcgcgcatcg gccgcgtggc aagcatcaaa cggcgaggat actggggctg cgctggcact
     2401 ggcccttgcc ggcgaccgcg cccgtccacg gaagttcgcc gatgatttgg caagaaggcc
     2461 tccgcaggat gctgtcgacg cgtccaccat ccgcgccgct atcgcgctcg aagagaaaga
     2521 tccggcgaga gccatcgcgg atcttcaggg ggcttcgcct ttcgaactcg gagatgccaa
     2581 cgagttaacg ctttatcccg tatacgttcg cggccaagcc tacctggcgg cgcatcacgg
     2641 cgcggcagcc gctgcggaat tccagaaaat cctcgaccac cccggcctcg cgttgaacga
     2701 gataatcttt ccgctggcgc gtctaggact gggccgggca cgagttctgg cgggcgacac
     2761 tcccagcgcg cgcaaggcct atgaggagtt ctttactctg tggcagcacg ctgatccgaa
     2821 cctgcccgtc cttctacagg ccaaagctga actcgcacga ttgcactaaa tacg
//
LOCUS       DASPUL010000112        62943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1523824, whole genome shotgun sequence.
ACCESSION   DASPUL010000112 DASPUL010000000
VERSION     DASPUL010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 62943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62943)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62943
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523824"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VEJ00_02585"
     CDS             <1..415
                     /locus_tag="VEJ00_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00074.1"
                     /translation="MRGAIEEEFLHWASPDAIAHHYSVGWRAVYRHAHATGLFEKRGR
                     NLRSALGHIIEHANHVPPTARDIIRSVRAYACLNENGKWTEPATRVIVSAGTKSAANR
                     RIAKATIQSGSSSRERAPRAEGPLLSSELPDNGVE"
     gene            549..1400
                     /locus_tag="VEJ00_02590"
     CDS             549..1400
                     /locus_tag="VEJ00_02590"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXX00075.1"
                     /translation="MREAFVLLLGLATGILVGLMGIGGGTVVVPAMVYLLGMDQHVAQ
                     GTSLLMQLLPLGLGAMWVYWKKGAVDLWAGIVCALGFLPGGYIGGRVALAISSHNLES
                     LFGLFLVFSAAMLVFEPGAKRAAARAAAGATDGSSGAAKRDSLASKAPRLTAFARDDN
                     PGRIVGVFAMSVFVGIASGLFGIGGGVLIVPLLVLLFGFEQHRAQGTSLIALVPPTGL
                     FACLTYYHAHQVDVKVAALLTPGFVLGGVLGGRLATRLASRTMRLVFAVFLLLVGAFA
                     AASGWMR"
     gene            1628..2413
                     /locus_tag="VEJ00_02595"
     CDS             1628..2413
                     /locus_tag="VEJ00_02595"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HXX00076.1"
                     /translation="MIDIHCHILPGIDDGADSLDTSCAMAEMAIADGITHVIGTPHAH
                     SSYKFDVERNLKLRDELQARFEGRIKFATGCDFHMSFENLQEIRHDAPRFTLNQKNYL
                     LVEFADYSIPATLDQALHELQLAGLRPIVTHPERNPLIRAKPERLYQWLRQGCYVQVT
                     AQSLLGKWGEAAQEMTNVWLDAGAIHFIASDAHNVTTRPLRLKETFDLVAQTRGEDVA
                     RALLVDNPLAAFEGRPLPWVPELPDDADMNGVAIHKKKRFWFF"
     gene            complement(2467..3384)
                     /locus_tag="VEJ00_02600"
     CDS             complement(2467..3384)
                     /locus_tag="VEJ00_02600"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HXX00077.1"
                     /translation="MAPAPITRIALVGFGEVGGIFGPDFAKQGIDVSVYDILFHSPES
                     REAMLAKARDCQVRACESLAECMHNADLVISAVTASSSLDVARDASKFLRVGQFFMDI
                     NSVSPGTKRADVAHVERSGAHFVESAVMAPVPPYRLKVQMLLGGRRAAELAPRLNAIG
                     MNATAVSETIGVASAVKMCRSVMIKGIEALAVECLFAARHYGAEEAVLASLDATYPNM
                     GWREKLPDYLVSRVAEHGRRRAEEMREVAQALRDVGVDPMLAEATAERQDWLVDEMAD
                     QNLNYKDLAPFSWIALAEALSKSASAKKK"
     gene            complement(3392..3700)
                     /locus_tag="VEJ00_02605"
                     /pseudo
     CDS             complement(3392..3700)
                     /locus_tag="VEJ00_02605"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
     gene            complement(3716..3838)
                     /locus_tag="VEJ00_02610"
     CDS             complement(3716..3838)
                     /locus_tag="VEJ00_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00078.1"
                     /translation="MSDLTITEILAAESACRALCVDYCEIVDAQDYSRLREIFA"
     gene            complement(4244..4963)
                     /locus_tag="VEJ00_02615"
     CDS             complement(4244..4963)
                     /locus_tag="VEJ00_02615"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HXX00079.1"
                     /translation="MSKSQEGLPGRRLVEPGKIVIRKVELPPADAVAALEQYGVSTVH
                     EAQGRIGLLASYMRPIYPGAAIAGQAVTVSLPPGDNLMIHVAVEVCTRGSILVVAPTS
                     PCTDGYFGELLATSLRAHGVKGLVIEAGVRDVKPLTDMKFPVWSRAISSQGTVKATLG
                     SVNVPIVCAGASIDPGDVIVADDDGVVVVKRADAAKVAAASKQRVDKEEGTRARLAKG
                     ELGLDIYSLRAKLKELGLEYQ"
     gene            5785..6771
                     /locus_tag="VEJ00_02620"
     CDS             5785..6771
                     /locus_tag="VEJ00_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00080.1"
                     /translation="MRERFGRRASVSAVVLCAIAMLLGQPGRLAAAQATSSGKACDYA
                     CLTSMVDQYLAALVAHDSSRIAVTANVRFTENTIPLSLGEALWRTMSGIGSYKLYFAD
                     VNGGSAGIEATIRENGTPAILLLRLKVVDHKISEIEQLVHRNADDALTVEKLGQPNPV
                     WTQPLDPAQRIPRAEMIKIANSYFEGILHSSGDSVPFDLRCNRILDGDQDTNNPTAKG
                     WFDKGSFRPDSMNIRDNMNTHIWTYIHSIDPRRFVLVDEKIGIVLGMLMFNHPGNVEF
                     ADVPGVGKIPMPPITRRPSSVAAGEFFKIEGGKIRQIEGVTIALPYGAKPGW"
     gene            complement(6883..7890)
                     /locus_tag="VEJ00_02625"
     CDS             complement(6883..7890)
                     /locus_tag="VEJ00_02625"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXX00081.1"
                     /translation="MIIDSHQHFVRLAKHPEVEGFRAAQLTGKNPDSLPKITDADVAK
                     SLEQVLSLMVARNIDVAFASPRAKAMATHEGTVEQNAVWADLNNGLILRACQLNPGRF
                     VPVGMLGQHPSCPSEQVAKQIERWANEGFVAFNFDPDGTGGRWQNPPFTGKHNYPVFE
                     AAQALDVPLVLHGSESINKNFPHTAVQYLVADITAFVQLMMAKDLFRDFPRMRFLIPH
                     AGGALPCQIGRWRAIAKYNSWTSPDEILGNGNVFVDTCVYNNLNMRMLFDAVPLDNIL
                     FGSECDGAFPGVVNEETGDHYDNTRRYVDDSKLSSADKDRVYSANARRVYPRTSRWLK
                     A"
     gene            complement(7955..8539)
                     /locus_tag="VEJ00_02630"
     CDS             complement(7955..8539)
                     /locus_tag="VEJ00_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXX00082.1"
                     /translation="MSRDKAGANRPTVTLHVVSSLDGVIARKDNSVSWLEGYGDVYEK
                     GVSEDSAHAEAFVKTIGCFVLGSRTYEHALQLGWPYGDTPTIVITSRELPSTRKSVEF
                     YSGNLKRLVNEILAPRYGNVWIVGGAMLCQSFLRLGLVDEIRLSIAPILLGDGLRLFG
                     DSGSERRWHLKDVVAYKNGFVELLYKIQDAPPVD"
     gene            complement(8526..9278)
                     /locus_tag="VEJ00_02635"
     CDS             complement(8526..9278)
                     /locus_tag="VEJ00_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXX00083.1"
                     /translation="MVGHAVALKHVLLLPGLACDKEVWRHQSRRLSESAIVQIADYGS
                     SDSIKKMAESVLRIAPARFALAGHSMGGRIAFEIFRLVPERVTGLALLDTAYRPFAGG
                     DHGARERAERLRLVDVARRQGMRAMAREWVRNMVHAARLSDKVLIDSIVEMFARKTPE
                     IFAGQINALLNRPDATPILATIACPTLVLCGRDDSWSTYATHKDIAARIPHSKLVAIE
                     NAGHMAPMERPKQVTAALVDWYSAVVREYVAR"
     gene            9675..9878
                     /locus_tag="VEJ00_02640"
     CDS             9675..9878
                     /locus_tag="VEJ00_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXX00084.1"
                     /translation="MREQGTVKWFNASKGFGFIQRMSGEDVFVHFSAIQSEGYKSLND
                     GQAVEFEVTKGPKGLQASNVSAL"
     gene            9947..10177
                     /locus_tag="VEJ00_02645"
     CDS             9947..10177
                     /locus_tag="VEJ00_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00085.1"
                     /translation="MKALQKGAYIKHFQYGLGVVTESDNDRTSIDFDLHGMKKFVTAL
                     MVVEFAEGTPPPRRKRARKKVAIVPAKPPTSK"
     gene            10192..10503
                     /locus_tag="VEJ00_02650"
     CDS             10192..10503
                     /locus_tag="VEJ00_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00086.1"
                     /translation="MQFVGFASTAGAREYRFTVREVSTEPREYVVTISNEAFSDHRLR
                     FQDAPDLCSIKLRHELAAHANHPVESHFHITDLELDDYRSSHSPRTPKTLYGRKPTDD
                     F"
     gene            complement(10669..11826)
                     /locus_tag="VEJ00_02655"
     CDS             complement(10669..11826)
                     /locus_tag="VEJ00_02655"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXX00087.1"
                     /translation="MFYRTDDLRIQSIEVVVAPAVLEQELPETDSASATIYQAREAIR
                     EILRGQEGLPGRRQGEPGRKEGLPGRRQGEPGRKDHRLLVVVGPCSIHDTKAARDYAQ
                     HLKAAIDELSQDLLIVMRVYFEKPRTTVGWKGLLNDPHLDQSFHINDGLRLARHLLLD
                     LAEMGVPAGTEFLDTFTPQYVAGLVSWGAIGARTTESQVHRQLVSGLSCPVGFKNGTS
                     GDTQIAVEAILSAAQPHTFLGHTKEGRSAIFVTTGNSDCHIILRGGRNTVNYTADAVA
                     RVCEQLKKAGLPERVMIDCSHANSNKDYRRQPIVCRDVAQQIATGGGHILGVMLESNL
                     VAGAQKLVPGQPLVYGQSITDACIGWDETLPLLRELASAVRSARSVRSRSF"
     gene            11973..12374
                     /locus_tag="VEJ00_02660"
     CDS             11973..12374
                     /locus_tag="VEJ00_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00088.1"
                     /translation="MNRRKMLSLLGASPAMLAGARIEAETSKKTNAKAGAAAGKIVAL
                     NPKGNPPPIQLVPMAKRLDTLDGKTIYLVSDGFPGADHFLNQIAIWFRKNMPSVTTIY
                     RLKAGQFADDDPKLNAEIKANGNAVIMAIGH"
     gene            12423..13700
                     /locus_tag="VEJ00_02665"
     CDS             12423..13700
                     /locus_tag="VEJ00_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HXX00089.1"
                     /translation="MGFPTAPMVTVAFKDLALSNAAQRGMPHERICFTPHPVWGKTDE
                     EMYAYLEGNDPVSGKPLMKEVIGALTNPLTAEESKTGEVSVSTGPETFADTEDNLQQY
                     YIDNGMTDFMQIVLPTEERVEAMLKGTSHKPDEVVGKMSPARGSYPDWSFTVRQVAIN
                     AVMAGAKPEYFPTVLAMAASGVTALSSSTNSFAVAAIINGPIRDKLNMNYGIGAMGPF
                     AQPNAAIGRAWTMLSRNLSNSGIPGQTYLGTLGNSVNYNNLIIPENEKASPWTPLSVQ
                     KGFKVDENVVSLFVGFSISQGQGGIGLGVKPVPQYHQAIAYNCTPFLPLFGALIVLDP
                     LVAQGLVELGFKTKEQLAEWLQKNQTISAKDARSTLTGGRWAPESIPDDQRVTYYQRP
                     DSYNFVVVGGQTNPYHMVANMSYRVSVSIDQWA"
     gene            13691..14293
                     /locus_tag="VEJ00_02670"
     CDS             13691..14293
                     /locus_tag="VEJ00_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00090.1"
                     /translation="MGVASARFFGRLATFAFLLQISTPVRGQLRPAAIDAHPLGTPVT
                     IIIEFGEQYLGSEQYDAKITVLEVVRGEKAWEMMKHASASNPAPKPGFEYLLARVRFE
                     FAARTSLSHHSYGLEPSQFAAAERDGTQFAAPSLAAPLVPALKGKLKSGDSVEGWLVF
                     LLVQGVSQPLMVFREDVGDVSHVGAGTWFELHKRAVAVKP"
     gene            14346..16226
                     /locus_tag="VEJ00_02675"
     CDS             14346..16226
                     /locus_tag="VEJ00_02675"
                     /inference="COORDINATES: protein motif:HMM:NF009232.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HXX00091.1"
                     /translation="MKHTSAVETTKPKTRVRAVRLRTSGASMERRADGSIIIRADEPL
                     AEYPRVLTERLIYWAGVVPDRPLAAKRDANGEWRYLTYSEALRKVRSLGQAFLSRGLS
                     ADRPVAILSENDLEHLLLMLAGQHVGVPTASIAPPYSLISKDFAKLRHVMKILAPGMV
                     FVSDADKYRRAIEAAVDPKIEVVCVQGEFSGRNWILFDELAATVPTDAVEAAHAAIQR
                     DGIAKFLFSSGSTGLPKAVINTHRMICSNQQMIRQVFPFLADEPPVMVDWMPWNHTAG
                     GNHNTGFPLYNGGTLYIDDGRPTHTGIAETVRNLREIAPTIYFNMPRGYDELLPHFRS
                     DAKLRENFFSRVKLLFYAGAALSQALWDAYRELMVEACGERIMMATGLGATETSPMAL
                     QCSWDTDQSGVIGIPISGVEAKLVPTGGKLECRVKGPNVTPGYWKEPELTRRAFDEEG
                     YYKFGDAVRFVDPEDVNKGLLFDGRLAEDFKLASATFVNVGPLRLKIIHWFAPYITDV
                     VIAGHDQNYIAVMLVPNLEACRTLAGLPKGSPASTILRRDTVTQKIGSLLESFAGQAT
                     GNSNRVERAMLLEEPPSLDAGEITDKGSLNQRVILDRRADLVEELYEESPSDRVLRIG
                     EN"
     gene            complement(16470..17555)
                     /locus_tag="VEJ00_02680"
     CDS             complement(16470..17555)
                     /locus_tag="VEJ00_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00092.1"
                     /translation="MRSGGGFRGGSFGGARSFGGGGYRGGTFGGARSFSGTGRGGAFS
                     GARSYSSRGWASRGFGRGFSAGRGATARPGIADGNFHSFGGSRGTTGGFAGARAGIAD
                     GAWHSFGSTRGTVGTGARGFNSGGFDRGFGGRGFGWGGRGWGGRGWGWGGWGWGLGFG
                     WGWPWGFGWWGPGWAWGPYWCDPYWWGGCPYYGYPVGYYEYGPPYAPPPEPAPDSDYG
                     DYYSYGPPYAAPPDNGDYYSNSAPTAAPQDQYSYDPGPDTGNVAESTPTVMLYLTDGT
                     MYPATDYWISGSTVHYVVAYGGEGTVPINQVDMRRTIDENAKHGVRFNLRPRSYSSPR
                     ASAPAAPASTPALKAQPIAQLQLQPAA"
     gene            complement(17846..18811)
                     /locus_tag="VEJ00_02685"
     CDS             complement(17846..18811)
                     /locus_tag="VEJ00_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXX00093.1"
                     /translation="MDAHAHRSTWKTLLAFAIIYLVWGSTYLAIRIGVREFPPLLMAA
                     LRFIVAGAVLYAWAIARGEQSPDRRQWSAIALLAVLFFVCDYGLLFWAEKCVPSGITA
                     VMMATIPLFIALCEITLLRTQTLTLRLALALLAGIAGVAVLASRSSFVGGAPVNPLGA
                     LALIFASLSWAVGTVLMRRLSLPSSKVMSSGTQMLAGGILLSIASAGFGEFGVFHPAA
                     ISRAAWFSLLYLIVAGSIVAFTAYLWLVHHESPTKVGTYAYVNPVVAVLIGYFFAGES
                     LGIRTILGTLLVLVSVVAITTARTAKPAASVSVEAGAKAATETVE"
     gene            complement(18936..19781)
                     /locus_tag="VEJ00_02690"
     CDS             complement(18936..19781)
                     /locus_tag="VEJ00_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXX00094.1"
                     /translation="MISSVESNGLRFDMFTGEEHFLAPNAVLISARHDAVLIDCGFVK
                     PDVEKLLGLVKQSGKHLRAICITHAHPDHYGGINDFAAAFPGAPILARQGVIDGMLEW
                     PAKRLHWQDMFGSLLPAGDIVYPKPLRGSEMYLEEREIIFIDSPIAETVHSTAFYVPS
                     ARVLIAGDLIFNRYHLYMSDTNNPDSWMNAIEQARGIGPIDTVFPGHGKFGGAEIFAD
                     MLRWLRDYRDVAKPGVHFTAIAKEMMRRYPNHGLPLLLWLSRGPGFGTAGAKELGVPA
                     ELLGG"
     gene            19933..20343
                     /locus_tag="VEJ00_02695"
     CDS             19933..20343
                     /locus_tag="VEJ00_02695"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HXX00095.1"
                     /translation="MRNRVLGGFIAAAIFLAAGTAWAHHSPSSIFDMTKPFTLSGTLT
                     QVEWVNPHIALYIDAKKADGSGVENWKWESAPPAFLRRVGVTSAQFKNAIGQTVSASG
                     FRAKDGSLYGFLTKITFADGTSLEMVAPGAADSR"
     gene            20370..21191
                     /locus_tag="VEJ00_02700"
     CDS             20370..21191
                     /locus_tag="VEJ00_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00096.1"
                     /translation="MRNQIGLAFAAAILLAIPSFAQQNATSTATGAVRMLNGHPDLSG
                     IWQYSVLVPGGGVKRSEASGTVDVKQIDLSGRMPAKVPVPGALPSQTAPSYKPEFQAK
                     VKYLFDNEAKLDKVFFCGKPGVPRLGPPRQIIQQPNEMIFLYEDMSGDTYRIVPTDGR
                     AHRADADPSYNGDSLGHWEGDTLVVDATNFVDDTWFGENGYFHTPAMHVIERFWRVGN
                     ELAYQVTIDDPNVLTQPWTEAPRLIKPSTEPLLESPACAEDDAKRMLNLDHHAQR"
     gene            complement(21218..22003)
                     /locus_tag="VEJ00_02705"
     CDS             complement(21218..22003)
                     /locus_tag="VEJ00_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00097.1"
                     /translation="MSKPHKEHRATPKQKGLDVGWYLAAVMAVGLSLAAPKIGQAATA
                     CGLVLMAAFLVHPIWTLSLVQRGRKRWLNFSSAMLVAACVLTTFGFWVWPPIRRHILS
                     EKERAWFDKALKETGSGDLEIQMACPPNDEQDCAYANQFISLIGRAGWNVNASVERIP
                     LTRPPIGVTVYRRGGNRDYSLKHWDAGGYFNINEPHLLAIQSAFRAIRIEIDGATDPD
                     VGEAVMMIYVGPEREDESQPTALTPATDWATGKRKGPFPFAQQ"
     gene            complement(22025..22798)
                     /locus_tag="VEJ00_02710"
     CDS             complement(22025..22798)
                     /locus_tag="VEJ00_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00098.1"
                     /translation="MKFFVTALLGLSVFGGASALAQGQAAQKTAGAPAAKSSDPTGHP
                     APYGPYPDWAGIWVLNFPKPGDPPPEVPSLTTSAAAQVKVFNEEDSKNVEPATDAANC
                     LPPGMPTIMSMPYDVEFLFTPGRVTIIQEAYMQVRRVFMDGRTHPAKLDPTFNGNSIG
                     HWEGGTLVIDTIGLGHNTPIGYSRLRHGPNLHIVERIRLVNPDLMEDRMTLTDPDVFT
                     QPWHTVHTFTRHREWDQLEYICEENNRNQIDKNGQVKPF"
     gene            22953..23351
                     /locus_tag="VEJ00_02715"
     CDS             22953..23351
                     /locus_tag="VEJ00_02715"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HXX00099.1"
                     /translation="MRKAVLSACMCALLLLAAGAAFAHHSFAMFDRSKETTLVGTVHE
                     FQWTNPHSWIELDVPNDSGGVDKWSIELNSPNNLSRQGWRSDSIKPGDKVSVVIWPLR
                     SGEKGGLFISLTLPDGSTLDEAAFRHRPKQ"
     gene            23452..23850
                     /locus_tag="VEJ00_02720"
     CDS             23452..23850
                     /locus_tag="VEJ00_02720"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HXX00100.1"
                     /translation="MRKISVVAWMGLVALVTAGTAAAHHSAAMFDRDKQVSLTGTIVQ
                     FEWTNPHSWIQIDVPNDSGGTDRWSVECNSPNNLVRQGWKSTSLKPGDKVTIVIHPLR
                     NGDKGGSFLSVTLPTGEVLTDPAYRAAPKY"
     gene            complement(23968..24771)
                     /locus_tag="VEJ00_02725"
     CDS             complement(23968..24771)
                     /locus_tag="VEJ00_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00101.1"
                     /translation="MIKSKWSIAGALVLAFSLGEFAPVARAQAQQSPQKKDIPEIPEG
                     ALAPNNLAKPRPKPPFNLTGTWTVDLSAGFSSFMFGPPYPKFKSEAQAAFDEAKKYAA
                     EHKPYRDDIGHCYPAGLPMLMTRVWPIAMIQLPTAIYMVSGFENGLRIIYLDGRPHTE
                     PDIVVRTFNGDSIGHWEGDTLVVDTTAMVSAHHWIDQGLPVSEDFHVIERMKLQDGGK
                     RLEIQYIMTDPKNWEGEWTSTKHWVRVDDTDITEVECTPDLNEHLLSTH"
     gene            24947..25432
                     /locus_tag="VEJ00_02730"
     CDS             24947..25432
                     /locus_tag="VEJ00_02730"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HXX00102.1"
                     /translation="MTEALQNFCDWLANTPLSLKIQTITWIIPAVQTIHILCVALVMS
                     CMAMMDLRLIGVAGTRRPVSVAVSRFVPWVWGALPVLLATGTILTIGEPSRELLNPYF
                     RTKMATLVVVILITFFVQRQNKKDANYWEARKGMAALAGVFSLLLWVGIVTCGRWIAY
                     Y"
     gene            25472..25963
                     /locus_tag="VEJ00_02735"
     CDS             25472..25963
                     /locus_tag="VEJ00_02735"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HXX00103.1"
                     /translation="MVIPEFCAWLENTALGTEIRQSTWMFPTIETLHVLATVMVVGSV
                     TMLDLRLLNLAYRDRSIREVHEDVLPWTWSSFAVAAVAGSLLFTSSATKYYHNFTFRM
                     KMLFLLLVGINAVFFELRTYPRVMDWDREARIPLAAKMAGAVGLLLWIGVVAFGRWTG
                     FTK"
     gene            complement(25993..26970)
                     /locus_tag="VEJ00_02740"
     CDS             complement(25993..26970)
                     /locus_tag="VEJ00_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00104.1"
                     /translation="MATPRNILAVAAILTFFSFAPAAARAQTTAPPADFDPNYNYDCD
                     RACLNVFVDELIAAMVAHDPSHLPLSREVKYTENGQLLKLGDGFWGTASGLPTYKIYA
                     DDPQAGQAMFMGVMPENGVPVIFCVRLKVELHRISEVEVIISRKEPGSLARPENLVTP
                     NPIFSETVPPAQRRSRQNMIAIANSYFNAIEKGHASFVPFDANCNRIESGVQTTNNAA
                     LATTPDSVLGMGCADQIKTRNFQPDTLIRDRRFAVIDEERGLVFVLGFFDHDATLRSY
                     TLADGRTIKQTRTAPWTWEIAEMFKIQDGKIMRVEALVNPAPYGMKAGW"
     gene            complement(27112..28101)
                     /locus_tag="VEJ00_02745"
     CDS             complement(27112..28101)
                     /locus_tag="VEJ00_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00105.1"
                     /translation="MIRRKIFAILVSALILGAVAVRSVGARPVPAKAADCDRACLNGF
                     VDQYMAAVAAHDPSKVPHSASVKYTENNVEMPIGEGLWQTSDGWGPYKVYVDDPQTGQ
                     VGFLGVANEDTHLSCFAARLKVVDNKVAEIEVIVARPDNPGSRGAGPIMGGPENLRDK
                     PLFSEDEPADQRVSRQKLVQLANGYFDTIQLNTGKIYTTFDPDCQRMENGFITANNPN
                     ATNPVAKMGCQAQLETGLLKIVTRCRDRRFVVDEQRQMVFVATFFDHNGTVRQNKLVD
                     GSVRTIGPPFDRPYTFLIFELFKVKDAKLRQIEAVLTTVPYYMPSPWVNYAKK"
     gene            complement(28253..29827)
                     /locus_tag="VEJ00_02750"
     CDS             complement(28253..29827)
                     /locus_tag="VEJ00_02750"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HXX00106.1"
                     /translation="MNRRNFMGTLAAAAGSTAAAGLPAVAGALSPFAPSANAETVHFG
                     NQSGFGGGSPFRCECEIRDCDIEGEIPSNIDGAFYRVGPDWQYPAPRGIPFDGEGHVS
                     MFRIQNGHVDFRSRYARTQRFKAQAEARRRLFNVYRNPFHDDPSVKGVSRGTANTHVV
                     YHHGLLFALKEDSPPVAMNPHTLETVDDYYTFGGGFHSLTHTAHPKFDRTTGEMISFG
                     YEAKGLATDDVYVFSANAKGQITWECWIKVPYVGMIHDFAVSQKYVAFLILPMATNVQ
                     RMQQGQVHFAWDSTLPTWFGVLERGGDGKDVRWFKGPERCATHVMGAFNDGNKIYVDM
                     DMALKNQFPFFPNLHGEPFDPKAASGTVHRLSVDLSDKTPRDYGMEELYPQSGALPRQ
                     DDRYQTIPYRIGFMPTTDPTKPVNPKLGNVAFRPTNSYTMFDHSTRKTREFWVGDDSS
                     LQECCFVPRSKDAPEGDGYLVGVANRLLEGGRSDLVIVDTAHLEDKPVATVKLPFRTY
                     SQVHGWWVSGDDLPKT"
     gene            complement(30386..31183)
                     /locus_tag="VEJ00_02755"
     CDS             complement(30386..31183)
                     /locus_tag="VEJ00_02755"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXX00107.1"
                     /translation="MPVTEGTKGSNARSDVRASEIMAAAGARELHDGEVVVVGLGLPE
                     VASVLARRTHAPRLTTMLETGVMNPSPKDTPVGLPDSRAFYRATCLSGFLDVMGMNLH
                     RGVVDVGFLGALELDRYGNINTTLVKDDSGKVRYVNGSAGGNDVASLAKRVIVIMRHE
                     KRKLPIAVTHLTSPGFAGGRSRQELNLRGGGPSRVITDMAVLGFDEKTHSMSVLSLHP
                     GIRVEELVENTGFPLHIPKDIPTTPLPTAEELRLLREEIDPKGVHLK"
     gene            complement(31223..32128)
                     /locus_tag="VEJ00_02760"
     CDS             complement(31223..32128)
                     /locus_tag="VEJ00_02760"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXX00108.1"
                     /translation="MADKIKTAAEVVRTIPSGSHIALGGFAIARNPIAVVHELIRQQK
                     QDLTLSQGVVGLDTDLLVGAGLVKHLIMGGGSLDRFGPAHCVNRARETKSLQCEDLTS
                     LTVCFRYLAGALGLSFIPIKSLFASEILERLEAGPGASDVKRSKCPFTGEEYVLLRAL
                     TPDVSFIHVQIADRDGNCQIDGARWTNEEQAKAARRLVVVAEELVPTEFIQRSPERTI
                     IPGHRVEAIIHQPFGAMPTSVFRCYDYDADHLRLYVEHAKDPRRVQEYLDKFVLGCRD
                     HWEYLEKLGGLRRMAELRAEPILGY"
     gene            complement(32315..33376)
                     /locus_tag="VEJ00_02765"
     CDS             complement(32315..33376)
                     /locus_tag="VEJ00_02765"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HXX00109.1"
                     /translation="MLRLPKFRYLQPKSLVEAVRMMSDGGPDALFVAGGTDLYPNMKR
                     RQQVPKVVIGLAQLKQLRTMKGKPAQGMVLGAGLTLTEVAEDRRIQRAYPAVSKAAEL
                     ISTPLLRNMGSIGGNLLLDTRCNYYDQNYEWRKGINFCMKKDGEICWVAPSSPRCWAV
                     QSSDSAPVMVAIGAQVKLVSTSEERLLPVAELYKDDGIHYLNKRPDELLTEIHLPPTN
                     GWRATYWKLRRRGSFDFPVLGVATCLRLAGDRTVEHARIVLGGVGSHPIEATLSEKAI
                     IGKKLTEETIHDAAEAAYSPVKPLDNTDFAMYWRKHMARYYVACTLRELGGLPQSLPH
                     PETENSVPALRVFSSGPFS"
     gene            complement(33451..35889)
                     /locus_tag="VEJ00_02770"
     CDS             complement(33451..35889)
                     /locus_tag="VEJ00_02770"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXX00110.1"
                     /translation="MGGANGAERKDLRVIGKPHRKVDAASKVTGQTKYADDLVFPRML
                     YTKILRSTVPHGRILKLDTSKAESYPGVKLVLLGKDLPIPYGALPVSEDEHPLAIGKV
                     RMMGDPVAAVAAVDEDVATEALDLIDVEYELLPAISSIQAALDTPEPRIHEYGEYGNI
                     HRRQVYEFGDVEVGFAEADFIREDITFYEGSTHLALEQHASVAQFGHDGKLTLWTCTQ
                     DPHYLHRSLAKVLGLPQNHIRVIATTNGGGFGGKCDPFAHEIIVSKLAMMTGRPVKIC
                     LTREEVFYCHRGRHPVLMSTKTGFKKDGTITAMHHRTFLDGGAYGGHGVASTFYTGVL
                     QPTTYKIKRYKFEAVRVFTNKAPCGPKRGHGTPQPRFAQEIQMDKIAEQLALDPAEMR
                     KKHLVPSNSLTVNWLRIGSMGLGKCIDKVVEGSKWRERFRKLPYGKGIGLACSGYMTG
                     AGTPIYWNSMPHSGVQLKLDRSGGVTVFCGSTEIGQGSDSILAYIVAEVLGIDPFDIR
                     VVTADTDLTPVDLGSYSSRVTLMTGNAAIQAAERAREMLIQAVSAKLEVPSDRLLFAD
                     RRVFDMENPEKGVTFVEAVWLAEAKFGTLGTVGSYKPAAAPGKFKGAGVGPSPAYSYS
                     AAVAEVEVNPETGVVVVPRIWMAHDIGQCINPVLAIGQIEGSIYMGLGEALMEEMTYR
                     GNRNVVHKFPSFLEYKSPTTMEMCEVITYLIEDRDSNGPFGAKEAGQGPQLPVPPAIA
                     NAIYDAVGVRIDEVPITPDKVLRAIRAKAKGQEGRYGPTVFPHCDMPASIFVPTPDEG
                     GDGKAVERPQPVLV"
     gene            complement(35876..36424)
                     /locus_tag="VEJ00_02775"
     CDS             complement(35876..36424)
                     /locus_tag="VEJ00_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXX00111.1"
                     /translation="MKPTKKRNNAKRAKAQICLRVNGEPVEVAFAPHKTLLEVLREDL
                     ALTGTKHGCELGECGCCAVLVDGRPVLSCLVLGVALEGHEVITVEGMAEGAKLHPLQE
                     AFAELGAAQCGYCTPGFLLTAKVLLEETPQPSRERIKKALAGNICRCTGYIKIFEAVE
                     LAAARMAGKPAEPSKESIYGWR"
     gene            complement(37500..38990)
                     /locus_tag="VEJ00_02780"
     CDS             complement(37500..38990)
                     /locus_tag="VEJ00_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HXX00112.1"
                     /translation="MSSSVVALTVISAIVAAGAFVGFLGGSHRKMDLEQWIVGGRGFG
                     LLLVWLLMAGEIYTTFTFLGASGWAYSRGAPVLYIIGYTCLAYVVSFYILPELWEAAR
                     RYRLQTQADFFQVRYNSKYLTAFVALVGMICIIPYLQLQITGLGIIVEVASFGVIHRT
                     PAMVISFAMVAGFVFTSGIRGVAWVSALKDLVLFFAAVFVGVAVPYIYFGGIGPMFAA
                     VARAQPGHLVMPGATTNLGHAWYVSTVLLCAFGFYMWPHFFGASFTAKSGDTLRRNAV
                     VMPLYIITMPLMFFVGLAALLVLPGLANGDLSLLMIVRKTFPSWFLGVIGGAGALTAM
                     VPAGILILTAASLFAKNLCRPILAPGMTDQQVGRLAKITVLAITCAALGSAIFGSTTL
                     VGLLLMGYAGVAQFFPGAVLGLYSKRVTISGVFAGMVTGVLIAVLLMLGKHDPIMGLN
                     AGFVALCFNFAVTGLLSWVTYTGESGFASESMSSPALQSRDNREPT"
     gene            40288..41463
                     /gene="cofG"
                     /locus_tag="VEJ00_02785"
     CDS             40288..41463
                     /gene="cofG"
                     /locus_tag="VEJ00_02785"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HXX00113.1"
                     /translation="MQGLVNTSQVMAALERGIEGAGVSREQALLLIEDAQLGSLLEAA
                     AAIRGRSKGRTVSYSKKIFIPLTHLCRDYCGYCTFRADPAVDGEPYMTSQEVLAVAEA
                     GRRAGCKEALFSLGDQPETIFPEARAFLRKQGFERTLEYLATMTQLVLDETGLLPHSN
                     PGIMDIGDLEWLKESNVSLGLMLESTSSRLRRPQGPHWKAPDKVPSLRLQTIERAGLL
                     SIAFTTGILIGIGETAEERIDSLLAIRDLHEKYGHIQEVIIQPFRAKPGTRMAREPEP
                     STEDLLRTLAIARLIFGSDMNIQSPPNLLSDDYPRLLGAGINDWGGISPLTKDFINPE
                     AAWPQISVLADKTAAAGFVLRERLAIYPEFSSRPRFVRAPLQSVLQKLQGEDGYARN"
     gene            41473..42720
                     /gene="cofH"
                     /locus_tag="VEJ00_02790"
     CDS             41473..42720
                     /gene="cofH"
                     /locus_tag="VEJ00_02790"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: protein motif:HMM:NF005609.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HXX00114.1"
                     /translation="MLNIQDPRWREISWEQAFKAANPTVADVLEKALAGRDLDASDGL
                     TLCGATDDDLLAVLKIANELRRRAVGDQVTYVVNRNLNFTNVCIVGCAFCGFGRGARS
                     PDAYFHSTESLVAKAVEAAERGATEVCIQGGLPRDLEGTYYADLLRTIKSHLPNLHLH
                     AYSPMEIVYGVEKTGVNLSEYLRTLKEAGLGSIPGTAAEILDDQVRKALSPNKLKVHQ
                     WINVIRTAHDLGIPSTSTMMYGHAELPEHWVRHLLLLREIQMDTGGFTEFVPLGFIHS
                     QTRLFRAGRARAGSSLAEHLLVHALARLLLNGHISNIQVSWVKLGFERALACLEAGAN
                     DFGGTLMEESISKSAGANFGESVLAGELRALIRTIDRTPAERTTTYKIRRVFKHADAE
                     ELLPLERMAAHREDPMLRDQVGY"
     gene            42720..43670
                     /gene="cofD"
                     /locus_tag="VEJ00_02795"
     CDS             42720..43670
                     /gene="cofD"
                     /locus_tag="VEJ00_02795"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HXX00115.1"
                     /translation="MDIVALAGGIGASKLLLGLHRVMDPRDLTVIVNTGDDIVLHGLS
                     ISPDLDIVTYTLAGLVNPDTGWGFRGETFRALEQLAVYGRPRWFRLGDRDLATHIHRS
                     DMLRAGARLTEVADSIREALGVQARILPMCEEPVPTIIDTGQGRLHFQEYLVERRAEP
                     IVRAISFDGIERAHPAPGVLEALESARSIVICPSNPLISIGPILAVPGVRDAMRKRRT
                     HVVAVCPIVGGKSLKGPSDQMLAQLGHDVSALGVARMYQDFCGTMVIDPIDSAQGPAI
                     ESLGVEVVIHPTVMRNLEDKELLARRIIQFTAKQKAGAAA"
     gene            43667..44341
                     /gene="cofC"
                     /locus_tag="VEJ00_02800"
     CDS             43667..44341
                     /gene="cofC"
                     /locus_tag="VEJ00_02800"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HXX00116.1"
                     /translation="MKAILIPVKEFHEAKKRLAPHFSPADRAALAEAMCEDFFSVVAA
                     TCCADRIFVITKEPGALSRARARGWETIVESHQTSESKSVDAGSRYCAEQGVRALLRL
                     PIDLPLVEPRDIEAVFEQLDVAPSVVMVPSSDGTGTNALLRSPAILFPSYFGPNSFPR
                     HLAEAKGCGARTRVFRNPRLELDIDELEDLRRLSNRLRPGSATARWLIERDVDLGAPR
                     ASAAGS"
     gene            complement(44338..45543)
                     /locus_tag="VEJ00_02805"
     CDS             complement(44338..45543)
                     /locus_tag="VEJ00_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXX00117.1"
                     /translation="MAEPAAKKAPLALDGVRVLALEVSVSGPHCSRILADMGAEVIKL
                     EKPGTGDLIRAWDSVVRGLSSGYVWLNGNKRSFAVDIKKPAGRQAVQRLAERVDVVLE
                     NFAPGVTDRLGLGAAELCARNPRLVYCSLSGYGQDGPYRDVKAYDLLIQGEAGIIATT
                     GYPDKPAKVGVPITDLASSMYATVGILLALYQRERTGRGQVIDISMFESALAWLGYFP
                     HHYWHRGEEPARVGMRHHYIVPYGPYMAGDGVYVSLAVATPQDWEVFCRKVARRPELL
                     EDARFRTAPDRRENRAMLEEMVEKLFLEQPHDEWMRRLGEAELPHGKLNGIGEVLAHP
                     QTIARKMIREMESPVGPVPVMGSPLRLSDSPARFDPIPALGQDTEPILRELGYTAAEI
                     AAFRRDQVI"
     gene            complement(45543..46043)
                     /locus_tag="VEJ00_02810"
     CDS             complement(45543..46043)
                     /locus_tag="VEJ00_02810"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXX00118.1"
                     /translation="MAVKPGWEGRFFEDFEVGDVYRCRLGRTVTENDNIWFTLLTNNT
                     NQIHFNNEYGKRTEFGKCLINSALTLAIVAGMGVADVSENGFALGWDEIKLPNPLFAG
                     DTLYSESEVLEKRESKSKPQWGIVKVRTRGIQQDGKVVIDYSRSVMVYKRAHAPKQNL
                     FPEVRP"
     gene            complement(46164..47063)
                     /locus_tag="VEJ00_02815"
     CDS             complement(46164..47063)
                     /locus_tag="VEJ00_02815"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HXX00119.1"
                     /translation="MRSWMFVPGNRQRMIEKSLGVAVDAIMMDIEDGVAPAEKDSARQ
                     QIATCLDQVAARLKAEPKLRTPARYVRINAVGHERSRDDLAAVVRKGLEGLVVPKVET
                     PEQIQSVAQVLDRREPEVGLQKGSVRLLCALESPKGLYNAYAIATASPRVIGLLFGAE
                     DFSRELSLPLRREGEARDLIYARSSLVTAAACAHVQSVDGVWPDLQDTEGLKTFSLQS
                     RRLGFSGMSLIHPAQIEIVNGAFTPAADEIDYARRVLKAFDEARTRGEGAVAFGGQLL
                     DLPIVDRARQTLELAESLGTRSS"
     gene            complement(47178..48335)
                     /locus_tag="VEJ00_02820"
     CDS             complement(47178..48335)
                     /locus_tag="VEJ00_02820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXX00120.1"
                     /translation="MGTTTEELRAIRENVAAICKDFPDTYWREVDKKEAYPQDFVTAL
                     TASGYLAALIPEQFGGAGLGIAEAGVILEEINRSGGNSGACHAQMYTMGTLLRHGSEE
                     QKQRYLPAIARGELRLQAFAVTEPNAGSETTRIETRAVRDGDRYVVNGQKIFISRAKQ
                     SDLMLLLARTTPYSDVQDKTQGISVFIVDMRNVKGNMQIRPLDMMVNHHTNTIFLEDV
                     EVPVENRIGEEGKGFRYIIDGWNAERILVASEAIGDGRWFIDRASTYARERVVFGKPI
                     GTNQGVQFPIAKAYAHVEAADLMRFDAAAKFDRKEKCGAEANLAKLLASEAAWEAADV
                     CLTTYGGYGFARDYDIERKFRETRLLSVAPVSNNLILAFLGQNVLKMPKSY"
     gene            complement(48373..48525)
                     /locus_tag="VEJ00_02825"
     CDS             complement(48373..48525)
                     /locus_tag="VEJ00_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00121.1"
                     /translation="MCDFAADAEAARQLLVGRRVLTVERARQLNAKDYFYQMLQDNPA
                     KLMWLD"
     gene            complement(48550..49551)
                     /locus_tag="VEJ00_02830"
     CDS             complement(48550..49551)
                     /locus_tag="VEJ00_02830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXX00122.1"
                     /translation="MLYDIELNSAAHYPVAGIVELSPLIEQAGFGAFWKGESNSTDPL
                     VLISGIAARTRTLKVGTAIYHIYGRSAVTLGIQSATLQDLSGGRLLLGLGVANKTIAA
                     WHGGTFDRPLRRAKEYIEIVRKTAAGERVEYQGEMYSTGQRFQLSWKPSHPTFPIYLA
                     GLGPQMTRLVGQISDGVFINMATPAKIREIADRVRAGAVEVGRDPSKIDIIAKVRVSL
                     HPDRAMARSRIRQVLTFYNIADHYKDMLRASGFEEEVNAVQEAFKAGGFKAASTKITD
                     AYMDKLPVIPATSIQEIKDRLKPFVEAGVTRLIVPYVPVTEPVIEDARRFVEAWGNS"
     gene            50188..51063
                     /locus_tag="VEJ00_02835"
     CDS             50188..51063
                     /locus_tag="VEJ00_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HXX00123.1"
                     /translation="MAREREEHVNTASRLLAVLNLFTIEKSEWTVEEAAKEIGVSVST
                     AYRYFRDLSKVGLLDSSPSAKYVLGPAVIENDRKIRLTDPLIKVGRPAMQRLVARSGS
                     AGVALLCRIYRNRVMCVHQEARVLPENTVGYERGRPMPMFRGASSKVIFANLPSRTAR
                     WFFEKYADEITAGGLGSDWETIKLNLRRIRRCGVYITRGEVDSGRVGIAAPVFGPDRS
                     VIGSITMAIPDGEATPQFVANISALVQAAAREIDAGLQFLSSGELVQDPGQPPVYHGL
                     SSSQDPGIADTSTGS"
     gene            complement(51225..52007)
                     /gene="cofE"
                     /locus_tag="VEJ00_02840"
     CDS             complement(51225..52007)
                     /gene="cofE"
                     /locus_tag="VEJ00_02840"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HXX00124.1"
                     /translation="MPDTETLKKPSPSLVQIIPLAGLPEVACGDDLARLLVEAAQRQR
                     LVIKQGDVLVVAQKVVSKAEGRIVSLESVKPSEQAEQWAAEYQKDPRVIEIVLREASS
                     IVRMERGVIIAKTRHGFVCANAGVDVSNVPSGTALLLPEDPDRSARTLQEQLARAFGV
                     HVAVIISDTFGRPWREGLVNVALGVAGMAPLVDYRGQRDTHGKTLQATVIALADELAA
                     AGGLIMGKINRVPAAVLQGLAISQGSGRGRDLIRPAERDLFR"
     gene            52230..52934
                     /gene="npdG"
                     /locus_tag="VEJ00_02845"
     CDS             52230..52934
                     /gene="npdG"
                     /locus_tag="VEJ00_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HXX00125.1"
                     /translation="MSPEKIAIIGGTGDLGFGLALRWARAGLQVMIGSRDETKAKEAA
                     QRIRDALDTHAPTESGRILISGLENAQATAQASVLVLAVPISAQVDILKSIRGSLGGA
                     ILVDATVALAAALGGKPTRTLRLWQGSAAAQARELVPATTPVLAAFHNVSADALRDLS
                     VTPECDILVCGDDAAAKQILFRLVNLIPGLRPIDAGPLEMSSIVESITALLISVNRRY
                     RVHHSGIRITGLELKT"
     gene            complement(52962..53225)
                     /locus_tag="VEJ00_02850"
     CDS             complement(52962..53225)
                     /locus_tag="VEJ00_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00126.1"
                     /translation="MFQFQTKSFACPESGPRKQGVENSVLPVCFVDNVSHCVEIKRGL
                     MSDNGQIDELVIPLARKHLLAALVQRGRHDKFDQTNVSADGFW"
     gene            complement(53764..53840)
                     /locus_tag="VEJ00_02855"
     tRNA            complement(53764..53840)
                     /locus_tag="VEJ00_02855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53804..53806),aa:Arg,seq:tcg)
     gene            complement(53985..54509)
                     /locus_tag="VEJ00_02860"
     CDS             complement(53985..54509)
                     /locus_tag="VEJ00_02860"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HXX00127.1"
                     /translation="MPSPPVIYGLFVDPESAERGMNALHRAEISPKKIVVMSAEPFDD
                     YSFSKMDHETPMPWIAAAGGLVGGMCGFLLARLTQVAYPIVTGGMPIFAAWPSGIVTY
                     ELTMLGAVLTTVVTLLITTKLPNWKPKLYDPEVSNGRILIGVLDPTDASRAEIENRLR
                     GAGAVKIKATSVHR"
     gene            complement(54511..55860)
                     /gene="nrfD"
                     /locus_tag="VEJ00_02865"
     CDS             complement(54511..55860)
                     /gene="nrfD"
                     /locus_tag="VEJ00_02865"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXX00128.1"
                     /translation="MATKSAEQITVDLLRPLSGLSGRYLLAATFFGGVVVAALCAFFY
                     QVYAGLGVSGLRTPVFWGFYITNFVFWIGLSHAGTLISAILRLANATWRRPVTRCAEV
                     ITVFALSIGATFPIIHLGRPWLAFWLFPYPSERGLWPNIRSPLLWDFFAINTYLMSSV
                     TFLLLPMVPDFALVRDRSEGLKKRIYGVVALGWTGTPKQWHRLETAMHIMAIAVIPVA
                     VSVHSIVSWDFAMAPVPMWHSTIFAPYFVAGAIFSGIAALILAMALLRKFLHLEEYLL
                     PIHFENLGKLLLVMSLLWFYFVFAERLTSWYGNESSEMAVFWTTQHGSYSPLFWTMVA
                     CNFLIPFPILAIKKLRTITGTAIASFTVVIGMWLERFLIIVPSLSHKYLTYSWGTYRP
                     TWVEITITAGTFCAMVLLYMIFSKIVPIISIWELKAGQHVKTHAVRLVVRSSESGAD"
     gene            complement(55841..56548)
                     /locus_tag="VEJ00_02870"
     CDS             complement(55841..56548)
                     /locus_tag="VEJ00_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXX00129.1"
                     /translation="MAHQWGMAIDLDRCTGCEACVVACHAENNISTVGEQQAAHSRAK
                     HWIRVERYYEGEFPDVTVKFRPVFCQQCDAAPCEPVCPTYASYHTSEGLNGQVYNRCI
                     GTRYCANACPYVVRYFNFFNPVWEKPLNLQLNPDVSVREVGVMEKCTFCVQRIKAGEI
                     AAEAEKRNLKDGEIQPACVQSCPTRAMVFGDLSDPESEVSRLAHSGRATKLLEEVGTR
                     PKVTYLPRDSWHGNEIG"
     gene            complement(56563..58812)
                     /locus_tag="VEJ00_02875"
     CDS             complement(56563..58812)
                     /locus_tag="VEJ00_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXX00130.1"
                     /translation="MERRDFLKISAVAGATAALEGCGNPDHQLIRFVPEEDLIPGIAA
                     WKPSICTLCPAGCGLLVRVMQGEAEVIRNGQLGLLKMGLAKKLEGNPAHPINQGKLCP
                     RGQAGLQVTYHPDRIKAPLARNGARGSGQFREVSWDDAIKQLVSQLSDLQASKSEGGL
                     AFVTSLLRGKRKTIVDGFTASFKKAIPVEFAFFDDAVIRAANSASFGHAQLPTVDLGQ
                     SNYLISFGADFLGTWNSPVAQSLGFGNMRKGRPGERGKFVQFEPRISQTGASADEWIP
                     VQLGAEGILALGLAHVVLAEKLRPASAAGHAGSLIEGWSQGLPDYAPEKIAAQTGIKA
                     ERIARIAREAAAHDPAVALIGDSAVAQSNGLFNALAVNALNALLGSVGKSGGISFAPA
                     PSPKESAVSLQTLASQITSGAAAVKVLLLYNANPVFAAPAAWGVRAALDKIPFIVSFG
                     SFIDDSSALADLILPDHSPLESWLDDVPSSGSAKAVVSVAPPAMNPLHNTRSMPDVLL
                     DVAHQLGGDSAKLLPWKTYDEAIQASFTALYKEKGSKAVKDADEFWIRAKESGGWWSA
                     EEAKAAPTGGASGISAPRTVAPKFDGDPSEYAFHFLPFASQLFYDGSVAHLPWMQEAP
                     DPLSTTMWGTWVEINPRTAEKLGIKQGDLVEVASQHGKLQAPALIAPGIAPDVLAMPV
                     GQGHENFTRYASGRGANPISILSPMAVEGTGSLAWAATRVKLTNVGRGKLILFGGSLT
                     EASPELKHR"
     gene            complement(58854..59423)
                     /locus_tag="VEJ00_02880"
     CDS             complement(58854..59423)
                     /locus_tag="VEJ00_02880"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXX00131.1"
                     /translation="MIGVLAVFAAILVKISSAQGGASPSGQSSDIFTPAHSTVSESIR
                     EFLGLRTKPVQPIAYTHKVHLGNGMQCTDCHVGVDQGPDARIPSVNFCMTCHQVIATD
                     KPEIRKLAAYRARGEDIPWERVYGFEPSAHVKFNHAPHIRAGVDCSACHGDMRQQTVA
                     ERKVDHTMGFCTNCHRQKNISVDCVTCHY"
     gene            complement(59728..61833)
                     /locus_tag="VEJ00_02885"
     CDS             complement(59728..61833)
                     /locus_tag="VEJ00_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00132.1"
                     /translation="MRALATCIVALGLAASPVLAKAGGTGANGTPSTTEAKSGAAPAQ
                     PGNTSLADPKANAAAKAEPSSSELESELQELRDLLESQSRQIQEQQQKMQLLEEQLTA
                     ATAAKEAVTADPADPESIGPKVALATKAVNAGAADDKKPETPNTINIKGISLTPIGFM
                     AAETVWRQKALAADVNTPLNSVPFNGSSNGHMSEFQASGRQSRFGMLVEGKLDKVKIG
                     GYYEADFLSAGTTSNNNQSNSYTLRQRQFWGQAAFNSGWTLTGGQQWSLLTETTKGMD
                     NRSEALPQVIDAQYVAGFSWARQFGFRVTKNFNNKVWLGVSVEEPQATLTVHGNPTVS
                     CAPANPFAGTPTTTGTCSASALNGTRVVTNVAAGAVSGNTVLLPNVSNDFLLGAFGAS
                     GGLYNPLGNYQYNPSPDFIVKAVFEPGFGHYEIFGVFAQFRDRVFPCAVTSTAAPVPG
                     CGTLPVSGGSVADTWSGAGAFNDSKTGGGVGGNARWALFEKHVDLGVHFLGGTGIGRY
                     GSVGLPDATIRWTTPVNPAVDGTLALLHNYQALGTVQLHPTPKLDINLYVGGEYSARA
                     GYIKSGATPNVGYGAQGFANWGCSAEIQPFAAQSTSASTGVPTSVAGGNGFIPGALQN
                     CTGDTRNLVEGSIQFWYRFYKGPKGTVQYGMQYSSYVRNTWRGVASGTGADGIAYSTN
                     GAPHANENMVFTSFRYVLP"
     gene            62219..62629
                     /locus_tag="VEJ00_02890"
     CDS             62219..62629
                     /locus_tag="VEJ00_02890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXX00133.1"
                     /translation="MRISQRGLYALQALTVLAQHPSGVVKIKDIAAEEQLPEKFLEAI
                     LLDLKRARIVTSLRGANGGYQLKRPAKKIALGEVIRRIDGPLAPFEDAESLRWRISSD
                     PKHRALFRILLDVRNATARILDHTSLADLCCSRN"
     gene            62756..62935
                     /locus_tag="VEJ00_02895"
     CDS             62756..62935
                     /locus_tag="VEJ00_02895"
                     /inference="COORDINATES: protein motif:HMM:NF021566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HXX00134.1"
                     /translation="MSAIATQDWQQDLSVTERELLREVALALRTIRYGSVTLTVHDGR
                     IVELNKTERVRKAPA"
BASE COUNT        12996 a        18216 c        19055 g        12676 t
ORIGIN      
        1 catgcgcggc gcgatcgaag aggaattcct ccattgggca agcccggacg cgatcgcgca
       61 tcactacagc gtcggatggc gcgccgtcta tcgccatgcc catgccaccg gactcttcga
      121 aaagcgcggc cgaaatctcc gttccgcgct tggtcatatc attgagcacg cgaaccacgt
      181 gcctcccacc gctcgcgaca tcatccgcag cgtccgcgcg tacgcttgcc tcaacgaaaa
      241 tggcaagtgg accgagcccg ccactcgcgt catcgtttct gctggcacga aatccgcggc
      301 caatcgccgc atcgccaaag cgacgatcca atcaggatcg tcgtcccgag agcgagcacc
      361 gcgagccgag ggacctctct tgagcagcga gctacccgac aatggtgtcg aataggatct
      421 actccaactt gctgaaagca aaggccggcc gcttcggcta cccgacaatt ttccgcgcct
      481 gcgctgaccc gctttccagc tgctcagccg gttgccgagg cgcgcgttct cggagaaact
      541 aatcggccat gcgagaagcg ttcgtactgc tgctggggct ggcgacgggg atactggtcg
      601 ggctgatggg gatcggcggc gggacggtgg tggtcccggc gatggtctac ctgctgggca
      661 tggaccagca cgtggcgcag ggaacgtcgc tgctgatgca actgctgccg ctggggctcg
      721 gcgcgatgtg ggtctactgg aagaaaggcg cggtggacct gtgggccgga atcgtctgcg
      781 cgctgggatt tctgccggga gggtacatcg gcggacgcgt cgcgctggcg atttcgtcac
      841 acaatttgga atcgctgttc gggctgtttc tggttttttc ggcggcgatg ctggtgttcg
      901 aaccgggggc caaacgcgcc gcggcgcgcg cggctgccgg cgcgacggat gggtcaagcg
      961 gcgcggcgaa acgcgattcg ctcgcgagca aggcccctcg gctcactgcg ttcgctcggg
     1021 acgacaatcc tggtcggatt gtcggggtct ttgcgatgtc ggtgttcgtc ggcatcgcga
     1081 gcgggctatt cggaatcggc ggaggcgtgc tgatcgtgcc gctgctggtg ctgctgtttg
     1141 gcttcgaaca gcatcgcgcg caggggacca gcctgatcgc gctggtgccg ccaacgggac
     1201 tcttcgcgtg cctcacgtat taccacgcgc atcaggtgga cgtgaaggtc gcggccctgc
     1261 tgacgccggg atttgtcctc ggcggagtgc tgggaggccg cttggccaca aggctcgcgt
     1321 cgcggacgat gcggctggtg ttcgcggtgt tcctgcttct ggtcggggcg ttcgcggcag
     1381 cctcgggctg gatgaggtag cgcgatgggc ggccgagctg ggcaagccaa gcgggatctt
     1441 cgggttaggg gcgaactggc gcattttccc gactgtcgaa gagcctgttg gaatcctgtg
     1501 gaaatacagc gcgcccaacg cgggagtaac tttcgcgcgg gcggggtcga tgcgcagcgg
     1561 ccgaagcgcg gggcccggct gcgttgtcat cgagtggtgc gtgcgatatc ctgaatcggc
     1621 cctcctgatg atcgacatcc actgccacat actgccgggg atcgacgacg gcgcggattc
     1681 gctcgacacg tcgtgcgcga tggccgaaat ggcgatcgcc gacggcatca cgcacgtgat
     1741 cggcacgccg cacgcgcatt cgagctacaa attcgacgtg gagcgcaatc tgaaattgcg
     1801 cgacgagctg caggcgcggt tcgaggggcg gatcaagttt gcgacgggct gcgacttcca
     1861 catgagcttc gagaatctgc aggagattcg tcacgacgcg ccgcgattca cgctgaatca
     1921 gaagaattat ctgctggtcg agtttgcgga ttattcgatt ccggcgacgc tcgatcaggc
     1981 gctgcacgaa ctgcaattgg cggggctgcg gccgatcgtc acgcatccgg agcgcaatcc
     2041 gctgatccgc gcgaagccgg agcggctgta tcaatggctg aggcagggat gctacgtgca
     2101 ggtgacggcg cagtcgctgc tcggcaaatg gggcgaggcg gcgcaggaga tgaccaacgt
     2161 gtggctcgac gcgggcgcga tccatttcat cgcgagcgac gcgcacaacg tgacgacgcg
     2221 gccgctgcgg ctgaaggaaa cgttcgatct ggtggcgcag acgcgcggag aagatgtggc
     2281 gcgggcgctg ctggtagata atccgctggc ggcgttcgaa gggcggccgc tgccgtgggt
     2341 gcccgagctg cccgacgacg cggacatgaa cggcgtcgcg atccacaaga aaaagcgatt
     2401 ctggtttttc tagattttct gcggcgacga gtcgtaatcc tcctcagctt cgtccgacgg
     2461 caagcgtcat ttctttttcg cagaagcgga tttgctgagc gcttcggcga gcgcgatcca
     2521 ggaaaacggc gcgagatctt tgtaattgag attctggtcg gccatttcgt cgacgagcca
     2581 atcctgccgc tctgcggtgg cctcggccaa catgggatcg acgccgacat cgcgcagcgc
     2641 ctgcgcgact tcgcgcattt cttcggcgcg gcggcggcca tgctcggcta cgcggctgac
     2701 caagtaatcg ggcagttttt cgcgccaacc catgttcgga tacgtggcgt cgagcgaggc
     2761 gaggacggcc tcttcggcac cgtagtgccg cgctgcgaaa agacattcga ccgcgagcgc
     2821 ctcgattcct ttgatcatca cgctgcggca catcttcacg gcggaggcga cgccgatagt
     2881 ttcgctgacg gcggtcgcgt tcatgccgat ggcgttgagg cgcggagcga gttcggctgc
     2941 gcgccggccg ccgagaagca tctggacttt aaggcgatac ggcggaacgg gcgccataac
     3001 ggcggactca acgaagtgcg ctccgctgcg ctcgacatgc gcgacgtcgg cgcgcttggt
     3061 gccgggcgag acggagttga tgtccatgaa gaactggccg acgcggagaa atttcgacgc
     3121 gtcgcgggcg acgtcgagcg atgaggatgc ggtgacggcg gagatgacga ggtcggcgtt
     3181 gtgcatgcac tcggcgaggc tttcgcacgc gcgcacctgg cagtcgcgcg cctttgcgag
     3241 catcgcttcg cgcgattcgg gcgaatggaa gaggatgtcg taaacggaga cgtcgattcc
     3301 ctgcttggca aaatcggggc cgaagatgcc gccgacttcg ccaaagccga ccagagcgat
     3361 tcgcgtgatg ggcgcaggag ccatcgcagc cctaagtatg aaacaaagtg cggccgcggc
     3421 gctcggcaat tctccaaccg gcggcggtgc ggatgtagcg gtcgacgtac attccgatca
     3481 actgcttggg cgcagcctgg cggccttcgg cggtttcggg ctcggatgca tcgcccatgt
     3541 agagaaggat gcggcaggag ccgctcgcgg cggtcggcga ttcgacgctc acgcaaatgt
     3601 tggtgacgaa gtgttgcgtc acgcgggtgc gaggccgcga ctcgaagctc gcgacaatgt
     3661 tttcgacgcc gcgaagcgga tcttgcggag ccgtggggcg ggcgaagacg gcgtcctagg
     3721 cgaagatttc gcggaggcgc gagtagtctt gcgcgtcgac gatctcgcag tagtcgacgc
     3781 agagagcgcg gcaggcggat tcggcggcga ggatttcggt gatggtcagg tcggacattt
     3841 gtcgccggct cggagaagga ttgtaactcg actacttact gccggtgagc agcgcatagg
     3901 tttggccctt cgggccagcc gtttttcgca gggctgaggc cctgcgcttc cacacaagag
     3961 gaaaccgtga ttcaaaaaca gattcaaccc atcctcacaa atcgagaatg gggcattcgt
     4021 cacccccagc taggcacagg caagagtgcc tgtgccacca aactcacttc ttgcacacga
     4081 gcaggcggct tgggccacga aattctagag cgggcgccct cgcgcgtttg agcgttaaga
     4141 gggattcttc gactgcgtaa ccggacgtcc ccaaagtcgg gacggtgaga cacgtccagt
     4201 tactccgctc agaatgacga tcaaatgggc cgccgcggca aggctattgg tattcgaggc
     4261 cgagctcttt gagtttggcg cggaggctgt agatgtcgag tccgagctcg cctttggcga
     4321 ggcgcgcgcg agtgccctct tctttgtcga cgcgctgctt ggacgcagcc gcgactttcg
     4381 cggcgtcggc gcgcttgacg acgacaacgc cgtcgtcgtc ggcgacgatc acgtcgcctg
     4441 gatcgatcga agcgccggca caaacgatcg ggacgttcac cgagccgagc gtcgctttca
     4501 ccgtgccctg cgaactgatg gcgcggctcc aaaccgggaa tttcatgtcc gtgagaggct
     4561 ttacgtcgcg gacgccagcc tcgatgacga ggcccttcac gccgtgggcg cgaagagatg
     4621 tggcgagcag ttcgccgaag tagccgtcgg tgcatggcga cgttggcgcg acgacaagga
     4681 tgctgccgcg cgtgcagact tcgacggcaa cgtgaatcat gagattgtcg ccgggaggca
     4741 gcgagaccgt gacggcttgg ccagcaatcg cagcgcccgg atagatcggg cgcatgtagg
     4801 acgccagcag gccgatgcgg ccttgcgctt cgtgaacagt cgagacgccg tattgctcga
     4861 gcgcggcgac ggcgtcggcc ggcggaagtt cgactttgcg gattactatt ttcccaggct
     4921 cgactaaccg tcgacctggg agcccttcct gtgacttgct catgaaatct cctcggtaat
     4981 gtgcggaaag gattctaggc agcgggaaag gttgcagtca actgaggatg caaagaggtg
     5041 gagaccaaaa tttcgattgg actgaaggtg gccttcgact cgactacttg ccgcccttcg
     5101 ccactgcgct caggataaac gggtgagaca gcgcattgga acggcccttc gggccggact
     5161 ttttcgcagg gctgcccttc gcttcgctca gggtaaaaag ccctgcgcct gtctgccgac
     5221 aggcaggctt ccaccgcgcg accgaccttt gtcggtcgca atgtcggagc taaagcttcg
     5281 accctctaaa agattcgatc gccgtgggct tcacgatgaa attcgcgcgc tgccggaagc
     5341 ccgggcctct aggctgtaag ccacgtaaag ttaagcttgc ctgcgctgag cggaaccaca
     5401 gggccacccc cgcattttgc gaagttgaat ttggcttagt cggtcgagac ttggatcaat
     5461 tgtgctgatc gctcatgccg ggagcacgcc ccgacttgtc ggggcggcaa acagcggcgc
     5521 tcgcgcgccg cactgcacat aggcctgtgc cgaagggcgc cggacggcca tgggcggcat
     5581 cgcgccaaga aagtgcgccg ggcgtagcgg tgctacgccc ctacagcagg agaacaattt
     5641 cgcggcgaga ggcccgttcg acatcactcg gggcaagccg gcgctacgac aacgaccgtc
     5701 ggcgcgcgct cgaggcgaat cttccgtggc acgtccgaaa cgggtacaat ccgttaaaaa
     5761 tttggcacgg aaggggaatc caaattgcgt gaaagattcg gcagacgtgc atccgtttcg
     5821 gcggtagtgc tgtgcgcgat tgccatgttg cttggccagc cagggcgcct cgcagccgca
     5881 caagcaacct cgtccggaaa agcctgcgac tacgcgtgcc tcacttcgat ggtcgatcag
     5941 tatctcgcgg ctcttgtggc ccacgattcg agccggatcg cggtgacggc gaacgtccga
     6001 ttcactgaaa acacgattcc gctaagtctc ggcgaagcgc tatggcgcac gatgagcggg
     6061 atcggcagct acaagcttta cttcgcggat gtgaatggag gctcggcagg aatcgaagca
     6121 acgattcgag agaacggcac accggcgatt ttgcttctgc ggctgaaagt tgtggaccac
     6181 aagatcagcg agatcgaaca gctggttcac cgaaatgccg acgacgctct gactgtcgaa
     6241 aaattgggcc agcccaatcc ggtttggact cagccgctcg accccgcaca gcgcattccg
     6301 cgcgccgaaa tgatcaagat cgcgaattcc tatttcgaag gcatccttca ttcctccggc
     6361 gattcggtgc cgttcgacct gagatgcaac cgcatcctcg acggagatca ggacacgaat
     6421 aacccgaccg cgaaaggctg gttcgacaag ggctcgttcc gtcccgactc catgaacatc
     6481 cgcgacaaca tgaacacgca catctggacc tacattcact cgatcgatcc gcggcgcttc
     6541 gtgctggtcg acgagaaaat cggaatcgtg ctgggcatgt tgatgttcaa tcatccggga
     6601 aacgtggagt ttgcggacgt gccgggcgtg gggaagattc ccatgccgcc cattacacgc
     6661 agaccgtcca gcgtcgcggc gggtgaattt ttcaagattg aaggcgggaa aatccgtcag
     6721 atcgaaggcg tcacaatcgc tctgccttac ggagcaaaac cgggctggta agctgcggcg
     6781 cgcgacaccg tgttcgcgcg gtgtcgttga actcgactag acgcactact ggagagccgg
     6841 acccagaatt cgcgccaggt ttcaatgctg ctcgcgaagc agctacgcct tgagccagcg
     6901 gctcgtgcgc ggatagacgc gccgggcgtt ggctgaatac acgcggtctt tgtctgcact
     6961 gctgagcttc gaatcgtcga cgtagcggcg cgtattgtcg tagtgatccc cggtctcctc
     7021 gttgaccacg cccgggaacg cgccgtcgca ttccgagccg aacagaatat tgtccagcgg
     7081 caccgcatcg aacagcatcc gcatgttcag attgttgtac acgcacgtat cgacgaacac
     7141 gttgccgttg ccgaggattt cgtcgggact ggtccagctg ttgtacttcg cgatcgctct
     7201 ccagcggccg atctggcacg gcagcgcacc accggcatgc gggatgagaa agcgcatacg
     7261 cgggaaatcg cggaagagat ccttcgccat catcagctgc acgaacgcgg tgatgtcggc
     7321 gaccagatac tgaacggccg tgtgcgggaa attcttgttg atcgactcgc tgccgtgcag
     7381 caccagcggc acatcaagcg cctgagctgc ttcgaagacc ggatagttgt gtttgccggt
     7441 gaagggagga ttttgccagc ggccgccggt gccgtcggga tcgaaattga acgcgacgaa
     7501 tccttcgttg gcccagcgct caatctgctt ggcgacctgc tctgaggggc aagaaggatg
     7561 ttgcccaagc atgccgaccg gcacgaagcg tccgggattc aactgacagg cgcgaaggat
     7621 cagaccgttg ttgagatcgg cccagacggc gttttgctcc accgtgcctt catgcgttgc
     7681 catggccttc gcgcggggag atgcgaacgc gacgtcgata ttgcgcgcga ccatcaagct
     7741 caagacctgc tcgaggctct tcgccacatc ggcatcggtg attttgggca gagaatcagg
     7801 attcttcccg gtgagctgcg ctgcgcgaaa gccttccact tccgggtgct ttgcgagacg
     7861 aacgaagtgc tggtgagagt cgatgatcat attcgccgaa agttgaaatc ctttctattg
     7921 ccggatgaaa ttctagaaaa gctacgggat tgcgtcaatc tacaggcggc gcgtcttgaa
     7981 tcttgtacag cagctcaaca aacccgttct tgtaagcgac cacgtccttc aaatgccatc
     8041 tcctctcgct gccggaatca ccgaacagac gaagcccgtc acccaagagg atcggcgcaa
     8101 tcgacaatcg gatttcatcc accaagccga gcctgagaaa actctggcat agcatcgccc
     8161 ctcccacgat ccagacgttt ccgtatctgg gcgcaaggat ctcattgacc aatcgtttga
     8221 gattgcccga atagaattcc acgctctttc tcgtggacgg cagttctctg cttgtgatca
     8281 cgatggtcgg agtatcgccg tagggccagc caagttgaag cgcgtgctcg taggtgcgag
     8341 aaccgagcac aaagcagccg atcgtcttga cgaacgcttc agcatgagcg ctgtcttcag
     8401 aaacaccctt ctcgtagacg tctccgtaac cctcgagcca agaaacgctg ttgtccttcc
     8461 tggcgatgac gccatccaga ctcgaaacca cgtgcagcgt tacggtgggc ctgtttgcgc
     8521 ctgccttatc gcgcgacata ttccctcacg actgcggaat accaatccac tagcgcggcg
     8581 gtcacctgct tggggcgctc catcggggcc atgtggccgg cgttttcgat cgcgacgagc
     8641 ttgctgtgcg ggatgcgagc ggcgatgtct ttgtgcgtgg cgtaagtgct ccaggaatcg
     8701 tcgcggccgc agaggacgag cgtggggcac gcgatcgtgg cgaggattgg agtggcgtcg
     8761 gggcgattga gcagggcgtt gatttggccg gcgaaaattt cgggagtttt gcgcgcgaac
     8821 atttcgacga tggagtcgat gagaactttg tcggacaggc gcgcggcgtg aaccatgttg
     8881 cgaacccatt ctcgagccat tgctcgcatg ccttgcctgc gggcaacgtc gacaaggcgc
     8941 agacgctcgg cgcgttcgcg ggcgccgtgg tcgccgccgg cgaagggacg gtacgcggtg
     9001 tcgagcagcg cgaggccggt gacgcgctcg ggaacgaggc ggaaaatttc gaaggcgatg
     9061 cgtccgccca tcgaatggcc ggccagtgcg aaacgcgcgg gcgcgatacg gagcacggat
     9121 tccgccattt tcttgatcga gtcgctcgag ccgtagtcag cgatttggac gattgcggat
     9181 tcggagaggc ggcgcgactg gtgtcgccag acttctttgt cgcaggcaag cccgggcagg
     9241 agcaggacgt gcttcagcgc cacagcgtgc ccgaccaatg ccgtcgcgcg acgcggacgt
     9301 tgaaatcgat tctggttcgg ataaagcctg gcacctgagc ttttctccgg tccgggtgcg
     9361 ccgggcggta cgtaaaatcg attcaaggtt attcggtgaa agggaggttg tcaacgcgcc
     9421 gcagtgtaac ggtgctgcgg attccgactg acaggcttgg ggagctacga atagtggcgc
     9481 agcctgtgcg caacggcgaa tatacaactg gacaatcggg cgcattcgtg tgaacatgta
     9541 gatgcgtttg ttgctcttgt aaaacctttg gaatcgttga aggggctgca agtccacact
     9601 tgcagcccct ctttttttgg ccggaggcgg tgggtaacgg caccaaatag ctcacacaga
     9661 aacgagatac acagttgaga gaacaaggaa cagttaagtg gttcaatgcc agcaaaggct
     9721 tcggattcat ccagcgcatg tccggcgagg acgttttcgt ccatttctcc gcgattcagt
     9781 cggagggata caagtcgctg aacgatgggc aagcggttga attcgaggtg accaagggcc
     9841 ccaagggcct gcaggcatcg aacgtctccg ccctttaacc ggcaaatgcg ggcccgccct
     9901 tcgggcgggc tcgcaagttt ttggcagtgc ggctcaagga gggttcgtga aggctcttca
     9961 gaagggcgcg tacatcaagc actttcagta tggtcttggc gtggttaccg aatccgacaa
    10021 cgatcgcacc tcgatcgact tcgatcttca tggcatgaag aaattcgtga ccgccctgat
    10081 ggtagtcgaa tttgcggagg gcacgccgcc gccacggcgg aagagagcgc gcaagaaggt
    10141 tgcgattgta cccgcgaaac caccaacctc caaatgagca aagagattct agtgcaattc
    10201 gttgggttcg cgtcgacagc gggcgcgcgc gaatacaggt tcaccgtgcg agaagtttct
    10261 accgagcccc gggaatatgt agtgacgatc tcgaacgaag ccttcagcga tcaccgattg
    10321 cgttttcaag acgcgccaga tctgtgctcg atcaagctgc gccacgaact ggcagctcac
    10381 gccaatcatc ctgtcgaaag ccactttcac attaccgacc tcgagcttga tgactatcgc
    10441 agcagccact cacctcgaac gcccaaaacg ttatacggcc gcaaacccac ggacgacttc
    10501 tagacgccag tgcgtgcggc gggaccgtac cgtcgccaga tcatgaatca gcgcggaact
    10561 tgcttgtggg caacttgtgt tttcagacca aggaaagttg cttgcgcgtt tctgctatgt
    10621 cccgctttgg tgtcacctaa agcgcgaagc cacccctgac ccggccgatc agaaactgcg
    10681 ggacctcacc gagcgggcgg agcggacggc agaggcgagc tcgcgaagaa gcggcagagt
    10741 ttcgtcccaa ccgatgcatg cgtcggtgat gctttggccg tagacgagcg gctggcccgg
    10801 gacgagcttc tgcgctccgg cgacgaggtt gctttcgagc atcacgccga ggatgtgtcc
    10861 gccgccggtt gcaatttgtt gcgcgacgtc gcggcagacg atgggctggc gtctgtagtc
    10921 cttgttgctg ttggcgtggc tgcagtcgat catgacgcgc tcgggaaggc cggccttctt
    10981 gagctgctcg cagacgcggg cgacggcgtc cgcggtgtag ttcacggtgt tgcgtccgcc
    11041 gcgcaggata atgtggcaat cggagttgcc ggtggtgacg aaaatagcgg agcgtccttc
    11101 tttcgtgtgg ccgaggaacg tgtgcggctg agcggcggag agaatcgctt cgacggcgat
    11161 ttgcgtgtcg ccggaagtgc cgttcttgaa tccgacggga cacgagaggc cggagacgag
    11221 ctggcgatgg acctggctct cggtggtgcg ggcgccgatg gctccccaac tgacgaggcc
    11281 ggcgacgtac tgcggagtga aggtgtcgag aaattccgtg ccggcgggga cgcccatttc
    11341 ggcgaggtcg agtaggagat ggcgcgcgag gcgcaggccg tcatttatgt ggaacgattg
    11401 atcgagatga ggatcgttga gcaggccctt ccagccgacc gtggtgcgag gcttttcgaa
    11461 atagacgcgc atgacgatga ggagatcttg cgaaagctcg tcgatggcgg ctttgaggtg
    11521 ctgggcgtag tcgcgggcgg ccttcgtatc gtgaatggag cacgggccga cgacgacgag
    11581 caggcgatgg tcttttctcc caggctcgcc ttgccggcgt cctgggagcc cttcctttct
    11641 cccaggctcg ccttgccggc gacctgggag cccttcctgg ccgcggagaa tttcgcgaat
    11701 cgcttcacgc gcttggtaga tggtcgccga agcggagtcc gtttcgggca gctcctgctc
    11761 gaggacggcg ggagcgacca cgacttcgat tgattgaatg cgaaggtcgt ctgtgcggta
    11821 gaacaaggcc gatacacatt actatagcag ggacgggcgc gagttgcggc ccagtatcat
    11881 tgacaaggct ggggcgcata actaacatcc agcgctgaaa gcacgcacgg gagttcgcgg
    11941 catttcatcg ggtttcgagg aaggggtgga cgatgaatcg acgcaagatg ctgtcgctgc
    12001 tcggcgcttc gccggcgatg ctggcgggcg cgcgaatcga ggcagaaact tcgaagaaaa
    12061 ccaacgcgaa agcgggcgcc gcggccggaa aaatcgtggc gctcaatccg aaaggcaacc
    12121 ctcccccgat ccagctcgtg ccgatggcca agcggctgga cacactcgac ggcaagacga
    12181 tttatctcgt gtccgacgga ttccccggcg ctgatcattt tttgaatcag atcgcaatct
    12241 ggttcaggaa aaacatgccg agcgtgacaa cgatctaccg gctgaaggcg ggccagttcg
    12301 cggacgacga tccgaagctc aatgcggaga tcaaggccaa cggcaacgcc gtgatcatgg
    12361 ccatcgggca ttgaagcacg tgcacaccag cgaccgtcgg tcactgcata aagtacgaac
    12421 agatgggatt tccgaccgca ccgatggtga cggtcgcgtt caaagacctc gcgctatcca
    12481 acgctgcgca acgcggcatg ccgcacgaaa gaatttgttt cactccgcat ccggtgtggg
    12541 gcaagacgga tgaggagatg tacgcctacc tcgaaggaaa cgatccggta agcggcaaac
    12601 cgctgatgaa ggaagtgatc ggcgcgctca ccaatcctct caccgcagaa gaatcgaaaa
    12661 cgggcgaagt gagcgtctcg acggggccgg agacgttcgc cgacaccgaa gacaatctgc
    12721 agcagtatta catcgacaac ggcatgaccg atttcatgca gatcgtgctg ccgaccgaag
    12781 aacgcgtcga ggcgatgctg aaaggcacga gccacaagcc agacgaagtg gtcggaaaga
    12841 tgtcgccggc aagagggtcg tatccggact ggtcgtttac agtgagacag gtggcgatca
    12901 acgcggtaat ggccggagcg aagccggaat attttccgac agtactcgcg atggcggcga
    12961 gcggagtgac ggcgctatcg agctcgacga attcgttcgc cgtggcggcg atcatcaacg
    13021 gaccgattcg cgacaagcta aacatgaatt acggaatcgg agcgatggga ccgttcgctc
    13081 agccgaacgc tgcaatcggg cgcgcgtgga cgatgctctc gcggaatttg agcaattcgg
    13141 gcatacccgg ccagacgtat ctcggcacgc ttgggaacag cgtgaactac aacaacctca
    13201 tcattcccga aaacgaaaag gcaagcccgt ggacgccact cagcgttcaa aagggattca
    13261 aagttgacga aaacgttgtg agcctttttg tcggcttcag catttcgcag gggcagggcg
    13321 gaatcgggct tggcgtgaag cccgtgccgc aataccacca ggcgattgcg tacaactgca
    13381 cgccgttcct gccgcttttc ggagcgctga tcgtgctcga tccgctggtt gcgcaggggt
    13441 tggtggagct gggcttcaag acgaaagaac agctcgccga gtggctgcaa aagaatcaga
    13501 cgatttcggc gaaggacgcg cgttcaactc tgaccggagg ccgatgggcg ccggaatcca
    13561 ttccggatga tcagcgtgtg acttactatc agcgacccga ttcgtacaac ttcgtcgtgg
    13621 tgggcgggca gacgaacccg tatcacatgg tcgccaacat gagttacaga gtgagcgtct
    13681 cgatcgatca atgggcgtag caagcgctcg atttttcggg cggctcgcta ctttcgcgtt
    13741 tctgcttcag ataagcactc ccgttcgcgg acaattgcgc ccagcggcga tcgacgcgca
    13801 tcctctcggc acacctgtaa cgatcatcat cgagttcggc gagcaatacc tcggcagcga
    13861 gcagtacgac gcgaagatta ctgtgctcga agttgtgcgc ggcgaaaagg catgggagat
    13921 gatgaagcac gcgagcgcgt cgaatccggc gccgaagccc ggattcgaat atttgctcgc
    13981 gcgggtgcga ttcgagtttg cggcgcgcac gtcgctttcg caccacagct acgggctcga
    14041 gccgagccag ttcgcggcgg cggagcgcga cggcacgcaa tttgcggcac cgtcacttgc
    14101 cgctccgctg gtgcctgctc tgaagggcaa gctgaaatcc ggcgattcgg tcgagggatg
    14161 gctcgttttt ctcttagtgc aaggagtttc gcagccgctg atggtctttc gggaagatgt
    14221 gggcgacgtg agccatgtag gcgcgggcac gtggttcgaa ctgcataagc gggccgtggc
    14281 cgtgaagccc tagcgcggcg aaagtttgcc gcgctacaat ttgatatcat ccaccggccc
    14341 gatccatgaa acacacctct gcggtcgaaa cgactaagcc gaaaacgcgc gtgcgagcgg
    14401 tgcgcctgcg aacatccggc gcatcgatgg agcggcgagc agacggctcg atcatcattc
    14461 gagcggacga gccgcttgcc gaatatccgc gcgttctcac ggagcggcta atttattggg
    14521 ctggtgttgt gcccgaccga ccgctggctg cgaagcggga cgcgaatggc gagtggcgat
    14581 atttgacgta cagcgaggcg ctgcgaaaag tgcgaagcct cggtcaggcg tttctgagcc
    14641 gaggcctttc ggcggaccgc ccggttgcaa tcctttccga gaacgacctc gaacatctgc
    14701 ttctgatgct ggccggtcag catgtggggg tgccgacggc gtcgattgca ccgccctatt
    14761 cgctgatctc gaaagatttc gcgaagctcc ggcacgtgat gaagatcttg gctcccggca
    14821 tggtgttcgt ttctgatgcc gacaaatacc ggcgcgcgat cgaagcagca gtggatccga
    14881 aaatcgaagt ggtctgcgtg cagggagaat tctcggggcg aaattggatt ttgttcgacg
    14941 agctcgccgc gacagttcct acagatgcgg tcgaggccgc gcacgctgca attcagcgcg
    15001 acggcatcgc aaaatttctg ttttcctctg gttcgacggg gctgccgaaa gccgtcatca
    15061 atacccatcg gatgatttgc agcaaccagc agatgatccg gcaggtgttt cctttcctcg
    15121 cggacgagcc gccggtgatg gtggactgga tgccgtggaa tcacaccgcc ggcggcaacc
    15181 acaacacggg gttcccgctc tataacggcg gcacgctcta tatcgacgat gggcgcccga
    15241 cccacacggg tatcgccgag acggtgagga atctccggga aatcgcgcct acgatctact
    15301 tcaacatgcc gcgcggttac gacgaactgc tgccgcattt ccgctcggac gcgaagctgc
    15361 gggagaattt tttcagccgc gtcaagctct tgttctacgc cggagccgcg ttgtcgcagg
    15421 cgctgtggga tgcctaccgc gaactaatgg tggaggcatg cggcgaacgg atcatgatgg
    15481 cgacggggct gggtgcgacg gaaacttcgc cgatggcgct gcagtgctcc tgggacacgg
    15541 accaatccgg cgtaattgga attccgatct cgggggtcga ggccaaactc gtgccgacgg
    15601 gggggaaact cgagtgccgc gtgaaaggcc cgaacgtcac gcccggttat tggaaggaac
    15661 cggaactcac gcgcagggcg ttcgacgagg agggctacta caaattcggc gacgcggtgc
    15721 ggttcgtcga cccggaggac gtcaacaagg gcctgctctt cgatggccgg ctggcggaag
    15781 atttcaagct cgcttcggcg acattcgtca acgtgggtcc gctgcggctg aaaatcattc
    15841 actggttcgc gccgtacatc accgacgtcg tgatcgcagg gcacgaccaa aactacatcg
    15901 ccgtgatgct cgtgccgaat ctggaggcat gccggaccct ggcgggcctg ccgaaaggaa
    15961 gtccggcgag cacgattctt cgccgcgata ccgtgacgca gaagattggc tcgctgcttg
    16021 aatcgtttgc cgggcaagcg actgggaatt cgaatcgcgt cgagagggcg atgttgcttg
    16081 aagagccgcc gtcgctcgac gcgggagaga ttaccgacaa gggctcgctc aatcagcgcg
    16141 tgatactgga tcggcgcgca gatctggtag aggaactgta cgaggagagt ccgtcagatc
    16201 gtgtgctgag gattggtgag aactagccgc gcctagacac cataggtcgt gtcctaaact
    16261 catttggatc gaaggaaaac accaacgcga ctacttgctg ccggtgaatc tgcgcatagg
    16321 atcggtgctt cgcgccgatc gttttgtcgg acctaaaagg tccgaccccc taaacgactc
    16381 ggccgaggtc gtcttgtagc gccccgagag aaaaagccca cccgatgcgg ttgacgcaaa
    16441 ggatgggcct tcgagattga gcttccagat taagcagcag gctgcagctg tagttgcgcg
    16501 atcggttgcg ctttcagcgc cggagtagat gcaggcgctg ccggcgcact tgctctcggg
    16561 ctgctgtaac ttctcggacg cagattaaac cgcacgccgt gcttcgcgtt ttcgtcgatc
    16621 gtgcgacgca tgtcgacctg attgatcggc accgttcctt cgccgccgta agcgaccacg
    16681 taatggaccg tgctacccga aatccagtag tccgtggccg gatacattgt gccatcggtc
    16741 aaatagagca tgacggtggg agtggactcg gcaacattgc cagtgtccgg gccgggatcg
    16801 taggaatact gatcctgcgg agcggccgtt ggcgctgagt tgctgtagta gtcgccgtta
    16861 tctggtggag ccgcgtacgg cgggccgtag gaatagtagt cgccatagtc cgagtcaggt
    16921 gcgggctcgg gcggcggagc gtatggtggg ccgtattcat agtagccgac cgggtagccg
    16981 tagtatgggc agcctcccca ccaatacgga tcacaccagt aagggcccca tgcccaaccg
    17041 ggtccccacc agccaaagcc ccaaggccag ccccagccga atccgagtcc ccaaccccaa
    17101 cctccccagc cccaaccgcg accgccccag ccgcgtccac cccatccgaa gccgcgaccg
    17161 ccgaagcctc gatcgaagcc gccgctattg aagccgcgtg ctccggtccc gactgtgccg
    17221 cgagtggatc cgaaggagtg ccaggcaccg tcggcgatac ccgcgcgcgc gccagcgaaa
    17281 ccgccggtcg tgcctcggga gccgccgaat gaatggaagt tgccgtccgc gattccggga
    17341 cgagcggtgg caccgcgccc ggcgctgaag ccgcgtccga atccgcgcga ggcccagccc
    17401 cgggatgagt agctgcgagc gccactgaac gcaccgcctc gacctgtgcc gctaaaggac
    17461 cgtgcgccac cgaaggtgcc gcctcggtag ccgcctccgc caaaggagcg ggcgccaccg
    17521 aagctgccac cgcggaaacc tccgccgctg cgcatagcgc taccgcctcg gaatccgcca
    17581 ccacctccgt ggaagccgcc gcctcccccg tgaaagccac cgccgccacc atgccctccg
    17641 ccgccatgtc tggctgctag agcaggattc ggtaggaaag ctagagtcag aattgcaatg
    17701 aaaggaatga ataccttaga aaggaaagat ttcagcatct gggtacacct ccatagaacc
    17761 aggccctgga gcggccccag cgattcacga cattctactc gattagataa ctggagggca
    17821 gcaaaggttt cccgaagggc ggcgctcact ccacggtctc agtggcagct ttcgccccgg
    17881 cttcgacgga aacagacgca gctggttttg cagtgcgcgc agttgtaatt gcgacgacgc
    17941 tgaccaagac cagcaacgtg ccaagaatcg tccgtatacc cagagattct cccgcgaaga
    18001 aatagccgat gagcacggcg acgacgggat tgacgtatgc gtaggtgccg actttcgtcg
    18061 gcgactcgtg gtggaccagc caaaggtacg ccgtgaacgc gacgatcgag ccggcgacga
    18121 tcagataaag cagcgagaac catgccgcgc gagaaatcgc cgcgggatga aaaacgccaa
    18181 attcgccgaa ccctgctgaa gcgatcgaca gcaagatgcc gccggccagc atttgcgttc
    18241 ccgagctcat gactttcgaa gacgggagcg aaagcctgcg catcagtacg gtcccgacgg
    18301 cccaactgag cgaggcaaag atcagcgcga gcgcaccgag tgggttgacg ggcgctccgc
    18361 ccacgaacga cgagcggctg gccaggacgg ctactcctgc gatgccagcc aagagcgcaa
    18421 gagcgaggcg gagcgtgagc gtctgcgtgc gcagaagcgt gatctcacac agcgcgatga
    18481 atagggggat cgtggccatc atgaccgcgg tgatacccga aggaacgcac ttctcggccc
    18541 agaaaagcag tccgtagtcg caaacgaaaa agaggacggc gagcagagcg atcgcgctcc
    18601 actgacggcg gtcgggcgat tgctcgccgc gcgcgatggc ccaggcgtaa agaacagcgc
    18661 cggcgacaat gaaccgcagt gccgccatga gaagcggcgg aaactcgcgg acgccgatgc
    18721 ggatggcgag atacgtcgag ccccagacga ggtagatgat ggcgaacgcg aggagcgtct
    18781 tccacgtgga gcgatgcgcg tgggcgtcca tgaccaatcc aggcgagcag tatgacccag
    18841 gatgaatccc cgccgataga gctacgccgg ggtcacaagc agccaatttg gaatgcgagc
    18901 ggactggctt ctgtgaagac gctcggcaga ttgtttcagc cgccaagaag ttcggcgggg
    18961 acgccgagct ccttcgctcc ggcggtgccg aagccggggc cacgagacag ccagaggagc
    19021 agcggcaaac cgtgattggg gtagcgccgc atcatttcct ttgcgatcgc ggtgaagtgc
    19081 acgccgggct tcgccacatc gcgatagtcc cgcagccagc ggagcatgtc ggcgaaaatt
    19141 tcggcgccgc cgaacttgcc gtggccgggg aaaacggtgt cgatcggacc gatgccgcgc
    19201 gcctgctcga tcgcgttcat ccacgaatcg gggttgttgg tgtccgacat gtagagatgg
    19261 tagcgattga agatgagatc gccggcgatc agtacgcgcg cggagggcac gtagaaagca
    19321 gtggagtgaa ccgtctccgc aatcggcgaa tcgatgaaaa tgatctcgcg ctcttcgaga
    19381 tacatttcgc tcccgcgaag cggcttggga tagacgatgt cgcctgcggg aagaaggctg
    19441 ccgaacatgt cctgccagtg aaggcgcttc gcaggccatt cgagcatgcc gtcgattacg
    19501 ccctgccggg caagaatcgg cgctccgggg aaagccgcag cgaaatcgtt gatgccgccg
    19561 tagtgatcag gatgcgcgtg cgtgatgcag atggcgcgga gatgtttgcc gctctgcttc
    19621 acgaggccga gaagcttttc gacgtcgggt ttcacgaagc cgcaatcgat gaggacggcg
    19681 tcgtggcgcg cagagatcag cacagcgttc ggcgcgagga aatgttcttc gccggtgaac
    19741 atatcgaaac gcaagccgtt ggattcgacc gaagagatca tgtgagcctc gggatgagca
    19801 agatcgcggc ggattctacc acgcgccgtg tccgcgaggg cggtctgcaa acgcgggagc
    19861 gccggcccgc agccagtgcg cttacaattc gggcttgtga tatgctcccg gaaatcggaa
    19921 aggaggccct cggtgcgaaa cagggtcttg ggtggattca tagcggctgc aatcttcttg
    19981 gcggcgggga ctgcatgggc acaccattcg ccgtcgtcca ttttcgacat gacgaagccg
    20041 tttacgctct cggggacgct gacgcaagtc gagtgggtga acccgcacat cgcgctttac
    20101 atcgacgcga agaaggccga tggaagcggc gtcgaaaact ggaaatggga gagtgcgcca
    20161 ccggcgttct tgcggcgagt cggagtgacc agtgcgcaat tcaagaatgc aatagggcag
    20221 acggtatccg cgagcgggtt ccgtgcgaag gatggatcgc tctacggatt cttgacgaaa
    20281 atcacttttg ccgatggaac gtcgctcgaa atggtcgcgc cgggcgcggc cgattctcgt
    20341 tagcttcgcg tgaggaagga ggccggaaca tgagaaacca aatcggcttg gcgttcgcgg
    20401 ctgcaattct tttggctatt ccctcattcg cccagcagaa tgcgacgagc acggcgacgg
    20461 gcgccgtgcg gatgttgaac ggtcatcctg atctgagcgg gatctggcag tactcggtgc
    20521 tcgtgcccgg cggcggcgtg aagagaagcg aagcgagcgg cacggtggac gtcaagcaga
    20581 tcgatctaag cggacgcatg ccggcgaagg tgccggtgcc cggcgcgcta ccatctcaga
    20641 cagcgccgtc ctacaagccc gaatttcaag cgaaggtgaa atatctcttc gacaacgaag
    20701 cgaagctcga caaagtgttt ttctgcggca agccgggcgt gccgcggctt ggccctcctc
    20761 gacaaatcat tcagcagcca aatgaaatga tctttcttta tgaggacatg tccggcgata
    20821 cgtaccgcat tgtgccgacc gacggacgtg cgcatcgagc tgacgccgat ccttcgtaca
    20881 acggagattc cctcgggcac tgggagggcg acacgctggt tgtggatgcc acgaattttg
    20941 tcgacgacac gtggttcggc gaaaacggct atttccatac gccggcgatg cacgtcatcg
    21001 agcgattctg gcgagtgggc aacgaactgg cgtatcaggt gaccatcgac gatccgaacg
    21061 tgctgacgca gccgtggacg gaagcgccgc gactgatcaa gccatccact gagccgttgc
    21121 tggaatcgcc cgcgtgcgcg gaagacgacg caaagcggat gctgaatctg gatcaccacg
    21181 cgcagaggta gggcatctcc aggagtgtgt ttgcagctca ctgctgcgcg aatgggaaag
    21241 gacctttacg ctttcccgtc gcccagtctg tcgcaggggt aagggctgta ggttgtgact
    21301 cgtcttctcg ttcgggtccg acgtaaatca tcatcacagc ttccccaaca tccggatcag
    21361 tggctccatc tatctcaatg cgtattgccc gaaaggcact ttgaatggca agcaagtgag
    21421 gttcgttaat gttgaagtaa ccgcccgcat cccagtgttt caacgagtag tctctgtttc
    21481 caccccgacg ataaactgtg actccgatcg gagggcgagt tagtgggatg cgctcaacac
    21541 tcgcattcac attccaccct gcgcgcccta tgagactgat gaactgattt gcgtacgcac
    21601 aatcttgttc gtcgtttgga ggacacgcca tctgaatttc tagatctccg cttcccgttt
    21661 ccttcaacgc cttgtcgaac cacgcgcgtt ctttttcgct cagaatatgt cgccttatgg
    21721 gaggccagac ccaaaagccg aacgtagtca gcacgcacgc ggcaacgagc atcgccgaag
    21781 aaaagttaag ccagcgttta cgaccgcgtt ggacgaggct cagcgtccaa attgggtgga
    21841 ccagaaatgc ggccattaag accaacccgc aagccgttgc ggcctgcccg atcttcggtg
    21901 ccgcaaggga gagaccgacg gccatgaccg cagcaagata ccagccgacg tccagccctt
    21961 tctgttttgg agtcgcgcga tgctctttgt gcggttttga catcaacaat agcgctagtt
    22021 tcgatcagaa tggtttgacc tggccgttct tgtcgatctg gttgcggttg ttttcttcgc
    22081 agatgtattc gagctgatcc cactcgcgat ggcgggtgaa cgtgtgaacg gtgtgccagg
    22141 gctgcgtgaa tacatcagga tcagtcaggg tcatgcggtc ttccatgaga tcgggattga
    22201 ccagacggat gcgttcaacg atgtgcagat tcgggccatg gcgcagacgg ctgtagccga
    22261 tcggcgtgtt gtggccgagt ccgatggtgt cgatcacgag cgtgccgcct tcccaatggc
    22321 cgatcgaatt gccgttgaaa gttggatcga gtttggccgg atgggttcgg ccgtccatga
    22381 agacgcggcg aacctgcatg taggcttcct ggatgatggt gacgcggccc ggcgtaaata
    22441 gaaattcgac gtcgtagggc atggacatga tcgtgggcat gccaggcgga aggcagttcg
    22501 cggcatcggt ggcaggctct acatttttcg agtcctcttc gttgaaaacc ttgacctgcg
    22561 ctgcggctga tgttgtgagc gaaggaactt cgggaggggg atcgcccggc ttgggaaagt
    22621 tgaggaccca gattcctgcc caatccgggt acggaccata cggggcagga tgtccggtcg
    22681 gatctgacga tttcgcggca ggcgcgccgg cagttttttg cgcagcctgc ccctgagcca
    22741 acgccgatgc gccaccgaaa acggacagac ccagcagtgc cgtaacgaaa aatttcatgt
    22801 tgtctccacc cagacgagaa gcgcggcgcc gaagttgccg cgaaagaagc gctacaatag
    22861 ttctgtcgta ttatagtact ttatgcgggc ggcgctaggc tgtgtcgggc cggccggagc
    22921 aaaatcgggt ctccgttgac gggggtggat cgatgaggaa ggccgttctt tcggcgtgca
    22981 tgtgcgcgct cttgctgctg gcagctgggg ccgcgtttgc gcaccactct ttcgcgatgt
    23041 tcgaccgttc gaaagagact accctcgttg gcacggttca cgaatttcag tggacgaatc
    23101 cgcacagctg gatcgagctc gatgtgccga acgacagcgg cggcgtcgac aagtggagca
    23161 tcgagctaaa cagccccaac aatctctcgc gccaaggatg gagatcggac agcatcaagc
    23221 cgggtgataa ggtctcggta gtcatctggc ctctccgaag cggtgaaaag ggcggactct
    23281 tcatatcgct gacgctaccg gacggctcga cgctggatga agcggctttc cgacaccggc
    23341 cgaagcaatg attggaacgt gaagttcggc ggattcgtct tatcagcact ggcaggaatt
    23401 ggggaacaga ttctcaaagc aacggaatcg ggtggagaac ggaggagtga gatgaggaag
    23461 atttcagtcg ttgcgtggat gggcttggtt gcgctggtga cggccgggac tgcggccgcg
    23521 catcactccg ccgcgatgtt cgaccgcgac aagcaggtct cgctcacggg cacgatcgtg
    23581 cagttcgagt ggaccaatcc gcacagctgg attcagatcg acgtgccgaa cgacagcggc
    23641 ggcacagatc gctggagcgt cgagtgcaac agccccaaca atctcgtgcg ccagggatgg
    23701 aaatccacga gcctcaagcc gggcgacaaa gtcacgatcg tgattcaccc gttgaggaac
    23761 ggcgataagg gcggaagctt tctctcggtg acgctcccaa ctggcgaggt gcttaccgat
    23821 cctgcctatc gcgcggcgcc gaaatactag attcgcgcga ccgtcaaagc ctagagcaga
    23881 ttcctcacct ccgtccccga gcgggatcgt cggggctggg ttcggaatga caccaagttt
    23941 cgcggaacgc tgtagttttt cctggcctta gtgcgtgctg agaagatgct cgttgaggtc
    24001 gggagtgcac tcgacctcgg tgatatcggt atcgtcgacg cgaacccaat gcttggtgct
    24061 ggtccactcc ccttcccagt tcttcgggtc ggtcatgatg tactgaattt cgagcctctt
    24121 gccgccatcc tgtagcttca tgcgctcgat tacgtgaaag tcctcgctga ccggcaggcc
    24181 ctgatcgatc cagtgatgcg cgctgaccat tgcagtcgtg tcgacgacga gtgtgtcgcc
    24241 ttcccagtga ccgatggagt cgccgttgaa cgtgcggact acgatatcgg gttcagtgtg
    24301 gggccggcca tcgagataga tgatgcgaag accgttttca aatccgctga ccatgtaaat
    24361 cgccgtggga agctgaatca tggcgatggg ccagacacgc gtcatcagca tcggcaggcc
    24421 ggcggggtag caatggccga tgtcgtcgcg atagggcttg tgctcggcgg cgtatttttt
    24481 cgcttcgtcg aaggccgctt gcgcctcgga cttgaacttc ggataaggcg ggccgaacat
    24541 gaacgagctg aatccggcgc taagatcgac ggtccaggtg ccggtgagat tgaacggcgg
    24601 cttcgggcgc ggcttcgcca ggttgttcgg cgcgagcgca ccttcgggaa tttccggaat
    24661 gtcctttttc tgcggcgatt gctgcgcctg ggctcgcgcg accggcgcga attcgccgag
    24721 agaaaacgca agcacgagtg cgcccgcgat gctccatttc gacttgatca tttgttccac
    24781 ccccaattga gagatcctta tcggaccgtc actttacgaa tcttcggcgc gcgcgtcaac
    24841 gccagactgg acgatttcag tcttttctgc gaacaaaagc atcgagcatt ccggtcgaca
    24901 gaggcggcgc aacagagtag tattttgggc gcaatcgggg gtggacttga ccgaagcgct
    24961 tcagaatttc tgcgactggc tggctaatac tccgcttagc ctgaagattc aaaccataac
    25021 gtggatcatt ccggcggtgc agactatcca tattctctgt gtggcgcttg tgatgtcgtg
    25081 catggcgatg atggatttgc gattgatcgg agtggcgggg acgcggaggc cggtgtcggt
    25141 ggcggttagc cggtttgtgc cgtgggtatg gggagcgctg ccagtgctgt tggcgacggg
    25201 caccatattg acgatcggcg agccgtcgcg cgagctgctg aatccatatt ttcggaccaa
    25261 gatggcgacc ctggtggtgg tgattctgat cacgtttttc gtgcagcggc aaaacaagaa
    25321 ggatgcgaat tactgggagg cgcgtaaggg gatggcagcg ctggccggcg tgttttcgtt
    25381 gctgctgtgg gtgggaatcg ttacctgcgg gcgctggatt gcttactact gattctgccg
    25441 cgttcgcggc taacggggga tcctcggttt gatggtcatt ccggaatttt gtgcgtggct
    25501 tgaaaacact gctctgggga cggagattcg acagtccacg tggatgtttc ccacaattga
    25561 gaccctgcat gtcctcgcga cggtgatggt ggtgggatcg gtgacgatgc tcgacctgcg
    25621 gctgctgaat cttgcgtatc gcgatcgcag cattcgcgaa gtgcacgagg acgtgctgcc
    25681 gtggacgtgg tcgagcttcg cggtcgccgc agttgcgggg tccctgctat ttacgtcgag
    25741 cgcgacgaag tactaccaca acttcacgtt tcggatgaag atgctgtttt tgctcctcgt
    25801 cggaattaat gcagtgttct tcgagttgcg gacgtacccc cgcgtcatgg attgggacag
    25861 ggaggcgcgc attcctctgg cggcgaaaat ggccggggcg gtgggcctgc tcctctggat
    25921 cggcgtagtg gcgtttggcc gatggaccgg cttcacgaag tagacagaac gccggtttcc
    25981 gcacctccgt cgctaccagc cggctttcat tccgtaaggc gcaggattga cgagcgcctc
    26041 aacgcgcatg atcttgccgt cctgaatctt gaacatctcg gcgatttccc aggtccaggg
    26101 agccgtgcgg gtctgcttga tggtgcggcc gtcggcgagc gtgtaagaac gaagcgtggc
    26161 gtcgtgatcg aaaaagccga gaacgaagac gaggccgcgc tcttcgtcaa tcacggcgaa
    26221 gcggcgatcg cggatcaaag tgtccggttg aaaattgcgc gtcttgatct ggtccgcgca
    26281 acccatgccg agaacggaat cgggggttgt ggcaagcgcg gcgttatttg tggtttggac
    26341 tccggattcg atgcgattgc agttggcatc aaacggaaca aatgacgcgt ggcccttttc
    26401 gatggcattg aagtagctgt tggcaatggc gatcatgttc tgacgcgagc gacgctgcgc
    26461 gggagggacg gtttcggaaa atatcggatt cggcgtgacg agattttcgg ggcgcgcaag
    26521 gctgccgggt tccttgcgcg aaatgatgac ttcgacttcg gagattcggt gtaattcgac
    26581 cttcaggcgt acgcagaaaa tcaccggcac gccgttttcc ggcatcacgc ccatgaacat
    26641 cgcctgtccg gcttgcggat cgtcggcgta gattttgtaa gtcggcaggc cgctcgcggt
    26701 gccccagaat ccatctccga gtttcagaag ctgcccgttt tcggtgtatt tcacttcccg
    26761 gctgagcggg agatgcgacg ggtcgtgcgc gaccatggcg gcgatcagtt cgtcgacgaa
    26821 gacattgagg catgcgcggt cgcaatcgta gttgtagttg ggatcaaaat cggctggagg
    26881 agcagtggtc tgcgcgcggg cggcggctgg cgcgaaggag aagaatgtca gaatcgcagc
    26941 gacggcgaga atgtttcgag gagttgccat agtgcctcac gtaagtggtt tttgttccgg
    27001 ggctagagcc caatctactt tgtcgcacag tttcgtgggg ctgaagcccc gcttccaccg
    27061 aaacgccaga aatagcctca ggtctcaaga cctgagctac atgaactcga actatttctt
    27121 ggcgtagttc acccacgggc tgggcatgta atacggaact gttgtcagaa ccgcttcgat
    27181 ctggcggagc tttgcgtcct tgaccttaaa cagctcgaag atgaggaatg tgtacgggcg
    27241 atcgaagggt ggaccgatgg tgcggacgct cccgtcgacc aacttgttct ggcggacggt
    27301 gccattgtga tcgaagaagg tggcgacgaa aaccatctgg cgctgctcgt cgacaacgaa
    27361 gcggcgatcg cggcagcgcg tgacgatttt gagcagcccg gtctcaagct gagcctggca
    27421 acccattttt gcgaccgggt tcgtcgcgtt gggattgttc gcggtgatga agccgttctc
    27481 catgcgctgg caatcgggat caaacgtcgt gtagatcttt cccgtgttga gttggatcgt
    27541 gtcgaaatag ccgttggcga gctgaactag cttttggcgg gagacccgct gatcggcggg
    27601 ctcgtcttcg ctgaacagcg gcttatcgcg gagattttcc ggaccgccca tgatgggccc
    27661 tgctccgcgc gagcccgggt tgtcgggacg cgcgacgatc acttcaattt ctgcgacttt
    27721 gttatcgaca actttgaggc gtgcggcaaa acaggatagg tgagtatcct cattcgcgac
    27781 gcccaggaag ccgacctggc ccgtttgagg atcgtcgacg tagactttgt atgggcccca
    27841 gccgtcggag gtctgccaca aaccctcgcc gatcggcatc tccacgttgt tctccgtgta
    27901 cttcacactc gcgctgtgcg ggaccttcga tggatcgtgc gcggccacgg cggccatgta
    27961 ttgatcgacg aatccgttga gacaagcgcg gtcgcagtct gcggctttgg ccggaactgg
    28021 cctcgcgcca actgagcgga cggcaacggc gccaaggatc agtgcagaca caagaattgc
    28081 gaaaattttc ctgcggatca tgctcccccc ggaatcccgt atcctagcgg ctcgacgcgg
    28141 cgaatctata ccagaacgca cgccgtcgca atcgccagtt cggccgagct gtattgccgt
    28201 cagcgcatca cgcgccgcgt cgacgaggag gcgcagtcga agccggaggg aatcaggttt
    28261 tcggcagatc gtcgccggag acccaccagc catggacctg gctgtaggtg cggaacggta
    28321 gcttcacagt cgcgaccggc ttatcttcga gatgcgccgt gtccacgatt accagatcgg
    28381 acctgccacc ttcgagcagg cggttcgcca cgccgacgag atagccgtca ccttcaggcg
    28441 cgtctttgct gcgcgggacg aagcagcatt cctgcaggct cgaatcgtcg ccgacccaga
    28501 actcgcgcgt cttgcgcgtg gagtggtcga acatcgtgta cgagtttgtc gggcggaatg
    28561 cgacgttgcc gagcttggga ttcacgggct tcgtcggatc ggtggtaggc atgaagccaa
    28621 tcctgtacgg aatcgtctga taacgatcat cctgacgcgg gagagcgccg ctctgcgggt
    28681 agagctcttc catgccgtag tcccgtggag tcttgtccga gagatcgacg gaaaggcgat
    28741 gcacggtccc cgacgccgct ttcgggtcga agggctcgcc gtggagattc gggaaaaacg
    28801 ggaactgatt cttcagagcc atatccatgt cgacgtaaat cttgttgcca tcgttgaacg
    28861 cacccatgac gtgcgtggcg cagcgttccg gtcctttgaa ccaacggaca tccttgccat
    28921 cgccgccgcg ctcgagcacg ccgaaccacg tgggcagcgt ggaatcccat gcgaaatgca
    28981 cctggccctg ctgcatgcgc tggacgttgg tggccatggg aaggatcagg aacgcgacgt
    29041 atttctgtga gaccgcgaag tcgtggatca tgccgacgta aggaactttg atccagcatt
    29101 cccaggtgat ctggcctttg gcgttcgcgg aaaaaacgta cacgtcgtcg gtggccaatc
    29161 ccttggcttc gtagccgaaa gagatcatct cgccggtcgt gcgatcaaat ttcggatggg
    29221 ccgtgtgcgt gaggctgtgg aagccgccgc cgaacgtgta gtaatcgtcc actgtttcga
    29281 gagtgtgagg attcatcgcg acgggagggc tatcttcctt gagcgcgaag agcaggccgt
    29341 ggtgatagac gacatgcgtg ttcgcagtgc cacggctgac gcccttgaca ctcggatcgt
    29401 cgtggaaagg attgcgatac acgttgaata gtcgcctgcg cgcctctgct tgcgctttga
    29461 atcgttgcgt gcgtgcgtag cgtgaacgga aatcgacgtg cccattctgg atgcgaaaca
    29521 tgctgacgtg tccctcgccg tcaaatggaa tgccgcgagg cgccggatac tgccagtcgg
    29581 ggccgacgcg ataaaacgcg ccgtcaatat tcgagggaat ttcgccttcg atgtcgcaat
    29641 cgcggatctc gcattcgcag cggaatgggc tgccgcctcc gaagccggac tgattgccga
    29701 agtgaactgt ttcggcattc gcagacggcg cgaacggaga gagagcgccc gccacggcgg
    29761 gcaggcccgc agcggccgtc gagccggccg cagccgccaa cgtacccata aaatttcggc
    29821 gattcatcgc cgctccactt tgagcttttt tcgattgctt ggatctcttt cgcatgtgtg
    29881 gtcacccccc ggaagcgtcg caagtcgcgc gatagtaggt agcacacccg cggcggaact
    29941 tacaagcaag atgcggcgtt gggctggaat tagacttgcc tcgagaacgg cacaagaata
    30001 ggcaggtctc acatcgctgg ttgggcgaga ctggcgcgcc ctaggttggg ggttgcagtc
    30061 gctccagctc ggcgcggtct atcgcgattc gctccgcgaa cgcagcaata aattctgtcc
    30121 ccctagcgat gttcacgtga tcgccgcgga agagcggcgc gaacggaggc tcgagcaggt
    30181 agctctctag aactccgtgt aggatattac tggactgttc gggactcaga cgggcgcgga
    30241 ttcgcaacgg ggacgtcgtc gggagattcc acaacgaaac agggatgtcg gtagcgaagc
    30301 gagttgccga ggatcagaca ggccgcgtgt tagtgggaat cttcgctttc ggtgcagtgg
    30361 cggcaggccg acaaagcctg ctcgctcact tcaaatgcac ccctttcggg tcgatttcct
    30421 ctcgcagcag acgcaactcc tcggcagtag gaagaggcgt cgtaggaatg tctttcggga
    30481 tgtgcaatgg gaatcccgta ttttccacca gctcttccac tcgaatcccc ggatgcaatg
    30541 agagcactga cattgagtgt gtcttctcat caaatccgag aacggccatg tcagtaatca
    30601 cgcgactggg cccgccgccg cgcaaattca attcctggcg gctgcgccca cctgcgaagc
    30661 cgggactcgt gaggtgagtc acggcgatgg gtaactttct tttctcatgg cgcatgatca
    30721 caatcacgcg cttggccagc gaggcaacat catttcctcc cgcgctgcca ttcacatacc
    30781 gcaccttccc ggaatcatct ttcacaagag tcgtattaat gttgccgtac cggtccagct
    30841 ccaaagcgcc cagaaaccct acgtcgacaa caccccggtg taaattcatc cccatcacat
    30901 cgagaaaacc gctcaagcac gtggctcggt aaaacgcccg ggagtccggc aaccccaccg
    30961 gtgtgtcctt cggactcgga ttcatcactc cggtctcaag catagtcgtg agcctgggcg
    31021 cgtgtgtacg tctggcaagc acgctggcaa cttcgggcaa tccaaggccc acgacgacca
    31081 cttcaccatc atgaagttcc cgcgccccgg cagcggccat gatttcgctg gcacggacat
    31141 cggaccgtgc attgctgcct ttcgttcctt cggtcaccgg catcgaacga atccgtcctt
    31201 tctacgctgc gctctatccc ggtcagtaac ccaagattgg ctcagctctc agttccgcca
    31261 tgcgccgtag tccgccaagc ttctccaggt attcccagtg atcgcggcag ccgagaacaa
    31321 atttgtccaa gtattcttga acgcgccggg ggtctttcgc gtgttccacg tacaggcgca
    31381 gatgatcggc atcgtaatcg tagcagcgaa aaaccgaggt cggcatcgca ccaaacggtt
    31441 gatgaatgat cgcctccact cggtgaccgg gtatgattgt ccgctcaggc gagcgctgga
    31501 taaactcggt gggaacaagc tcctccgcca ctacaaccaa ccggcgggca gccttagctt
    31561 gctcctcgtt agtccagcgg gctccgtcga tctgacaatt tccatcgcgg tccgcgatct
    31621 gcacatggat gaaggagacg tccggcgtaa gggccctcaa caagacgtac tcctccccgg
    31681 tgaagggaca ctttgatcgt ttcacatctg aagcacccgg ccccgcctct aatcgctcca
    31741 agatctcaga agcaaacaac gacttgatgg gaatgaagga gagacccagc gctcctgcca
    31801 aataccgaaa gcaaactgta aggctggtta agtcttcgca ttgtagcgac ttggtttcgc
    31861 gcgcgcggtt cacacagtgg gcggggccga atcggtccag cgatccccct cccatgatga
    31921 ggtgcttcac cagtcccgct cccacaagca agtcggtatc gagccccaca actccctggg
    31981 agagcgtcaa atcctgcttc tgctggcgga tcaattcgtg gacgacggcg attgggttgc
    32041 gggcaatcgc gaaaccgccc aatgcaatgt ggcttcccga aggaatcgtg cgaacgactt
    32101 cggcagcggt tttgatcttg tcagccatac tcgaggtgcc agtctgtctg tcggtgcaga
    32161 cgaatcgtgg tggtgcatcg tcgcgagagc gcagagctta ccaagcagaa tggctaatcg
    32221 tcaacaagca cggcccatgg ataccgccat cgccatcttg gcaaggaaac atccgaatct
    32281 cgtttgtgcg gtgacggggc tcgtgttgga aggctcagga gaaggggccg ctcgaaaata
    32341 cacgcagggc cggcacgcta ttttcggttt ccggatgcgg taagctctgc gggaggccgc
    32401 ccagttctcg aagcgtgcag gcgacgtaat agcgcgccat gtgcttgcgc cagtacatgg
    32461 caaaatcggt gttgtcgagg ggttttacag gagagtacgc cgcctcggcg gcgtcatgga
    32521 tcgtttcttc cgtcagcttt ttcccaataa tggccttctc cgagagcgtc gcctcaatgg
    32581 gatgggagcc aacccctccc aaaacgatcc tagcgtgctc caccgtccga tcgcccgcca
    32641 ggcgcaggca agtggccacg ccgaggaccg ggaaatcgaa cgaaccacgg cggcgaagct
    32701 tccaatacgt cgctcgccag ccgttcgtcg gcggcagatg gatttcggtc agcagttcgt
    32761 cgggccgttt attgaggtag tggattccgt catccttata aagttccgcc accggcagca
    32821 gtctttcttc cgaagtcgaa accagcttca cttgcgctcc gatggctacc atcacagggg
    32881 cggagtcgct cgactgcacg gcccagcagc gcgggctgga gggagccacc cagcagatct
    32941 cgccgtcctt tttcatgcaa aagttaattc ccttgcgcca ctcgtagttc tggtcgtaat
    33001 aattgcagcg cgtatcgagc agcaggtttc ctccaatgct acccatgttg cgcagcagcg
    33061 gcgtggagat caactcggca gccttcgaca cggccggata ggcacgctgg atgcggcggt
    33121 cctccgcaac ctcagtcaga gtcagacccg cccccaggac cattccctgc gctggcttgc
    33181 ccttcatcgt gcgcagttgc ttaagctgtg ccagcccgat gaccaccttc ggcacctgct
    33241 gccggcgctt catattggga tagagatccg taccgccggc tacaaacagg gcgtcaggcc
    33301 ctccgtccga catcatgcgg acggcctcga ccaaagactt gggttgcaga tagcgaaatt
    33361 tgggaagtct cagcattcta agtcccgctt agacagacat ctctgtggga acaatgacgc
    33421 acgcagcaat gcctgtctta cagcccgccg ttacaccaaa acaggctgcg gccgttctac
    33481 agccttgccg tcgccgcctt catccggcgt tggcacaaaa atcgaggcgg gcatgtcaca
    33541 gtgcgggaat acagtaggcc cgtatctgcc ttcctgcccc ttggccttcg cccgaatcgc
    33601 tctcagaact ttatccggtg tgatcggaac ctcgtcgatg cgaaccccta ccgcgtcata
    33661 aatcgcgttg gcgatcgcgg gcggaaccgg caactgcggt ccctgtccgg cttccttggc
    33721 cccgaacggg ccattggagt cccgatcctc gatcaaatag gtgataactt cacacatctc
    33781 cattgtggtg gggctcttgt attcgaggaa agaggggaac ttgtgaacca cgttgcggtt
    33841 cccgcggtag gtcatctcct ccatcaaggc ctcccccaag cccatgtaga tactgccttc
    33901 tatctgcccg atcgccagca ccggattgat gcactggccg atgtcgtgag ccatccagat
    33961 gcgggggacg acgacaacgc cagtttcggg attgacctct acttcagcca ccgccgcgga
    34021 atagctgtag gcaggtgaag gaccgactcc agctcccttg aactttccag gcgcggccgc
    34081 cggcttgtaa gaaccaaccg tgccgagcgt gccgaacttc gcttcggcaa gccacacggc
    34141 ctccacaaac gtcacgcctt tctccggatt ctccatgtcg aaaacgcggc gatctgcaaa
    34201 gagcagccgg tcggagggaa cctccaattt cgccgacaca gcctggatca acatctccct
    34261 ggcccgctcg gcggcctgga tagccgcgtt gccggtcata agggtgaccc ggctcgagta
    34321 actgccgagg tccacgggag ttagatcggt gtcggccgtg acgacccgaa tgtcaaaggg
    34381 atcgatccct aggacctcgg cgacgatata ggccaaaatc gaatcggaac cctgaccgat
    34441 ctctgttgag ccgcagaaga cggtcacgcc cccgctgcgg tccagtttca gctgcacgcc
    34501 ggagtgtggc atgctgttcc agtagatggg agtgcccgcc ccggtcatgt agccgctgca
    34561 cgccaacccg atgccttttc cgtaggggag tttgcggaag cgttccctcc acttggagcc
    34621 ttccacgacc ttgtcgatgc acttgcccaa gcccatgctg ccgatgcgaa gccaattcac
    34681 tgtcagcgaa ttggacggga ccaggtgctt cttgcgcatt tcggccggat cgagcgccaa
    34741 ctgctccgcg atcttgtcca tttgaatttc ctgggcgaag cgtggctgag gagttccgtg
    34801 tccccgtttc gggccgcaag gcgctttgtt ggtgaacact cggacggctt caaacttgta
    34861 gcgcttgatc ttataggtcg tcggttgcag caccccggtg tagaacgtgc tggccacgcc
    34921 gtggcctccg taggcgccgc cgtccaaaaa cgtgcgatgg tgcatggccg tgatggtgcc
    34981 gtccttctta aacccggtct tggtggacat gagaacggga tgccgaccgc gatggcaata
    35041 gaagacctcc tcgcgcgtga ggcagatctt gacggggcgc cccgtcatca tcgccagttt
    35101 cgagacgata atttcatgcg caaagggatc gcactttcct ccgaagccgc ctccgttcgt
    35161 agtggcgatg acgcggatat gattctgcgg tagccccagc actttggcga gcgaccggtg
    35221 caggtagtgc gggtcctggg tgcaagtcca gagcgtgagc ttaccgtcat gaccaaactg
    35281 ggccacactg gcgtgttgct ccaacgccag atgtgtgctg ccctcataaa aagtgatgtc
    35341 ttcccggata aagtcggcct cggcaaagcc cacttccacg tcgccgaatt cgtaaacctg
    35401 tctccgatgg atattgccgt attccccata ttcatggatg cgaggctcgg gggtgtcgag
    35461 tgcggcctgt atcgaggaga tggccgggag gagttcgtac tcgacgtcaa tcaaatccag
    35521 cgcttcggtc gccacatctt catccactgc tgcgacggcg gcgaccggat ctcccatcat
    35581 gcggactttg ccaatcgcca gcgggtgttc atcttcgctg acgggcagag ccccatatgg
    35641 gatcggcagg tcttttccga ggagcaccag cttgacgcct ggatagcttt cggccttgga
    35701 cgtatcgagc ttcaggatgc ggccgtgcgg aactgtgctg cgcaaaatct tggtgtacag
    35761 catgcggggg aagacgaggt cgtcggcata cttggtctgg ccggtgacct tcgatgctgc
    35821 gtctaccttg cgatggggct ttcctatgac gcgcaggtct tttcgctcgg ctccgttagc
    35881 gccacccata aatactctct ttgctgggct cggccggctt gccagccatc cgcgcggcag
    35941 ccagctccac tgcttcgaat atcttgatgt agccggtgca gcggcaaatg tttccggcga
    36001 gagccttctt gatccgttcg cgggaaggct gcggggtttc ctccagcagc acttttgccg
    36061 tcaggagaaa tcccggtgtg caatatccgc actgggcagc cccgagttca gcaaacgcct
    36121 cctgcaaggg atggagcttc gctccctctg ccatgccttc gaccgtgatg acttcatgtc
    36181 cttccaaggc gacccccagg accaggcagg aaagtaccgg acgaccatcc acgagcacgg
    36241 cgcaacatcc gcattccccc aactcgcagc cgtgcttggt gccggtaagc gccaaatctt
    36301 cgcgaaggac ttccagaagg gttttatgcg gggcaaacgc cacctcgact ggctcaccat
    36361 tcacccgcag gcaaatctgc gccttcgccc gtttggcatt gtttcttttc ttggttggtt
    36421 tcattccttg ctcttagatt ttattcagag attccgcaac caaaccagtt caacagaatt
    36481 tcagcagcat ggtgatcagt ccgatttcca ttatggagtt tgggttcgcc cagtaagata
    36541 ctcccggccg tcgctgactg caactgtcgg ccaggattgt acggaacgct ggccgcaggg
    36601 atcatcgccg aaatgggcct gtggttgaca ccaaacgacc acattctgct ttgctggttt
    36661 ccgaacagac cggcttggtt gcgagcaggc gatgaaaatc actccgagaa gaacccgaat
    36721 tcgttgggcc aaagggtccc gcaaccgagc accgggtgcg gggtcaggac agatcgtgct
    36781 cctccaagtg ggctcagaag cacaaaaacc ttcagccagc gcagaactgt tctttcttga
    36841 ctcctgccct tatagagctt atcagttggt cgtataattc ccggaaacga ggtatcaact
    36901 cttcatgacc caaccgaata gaatccttta ccgcggcgtt ccgatgatag agggctggcc
    36961 tgagaaaatt atggctgctc aacaaattgt ggcgcttacg caggagcggc gagccattcc
    37021 tcgcattcgt tacggcgacg agcacgatga ctggggagta gataaaaacg cgtgccctga
    37081 ttgccgagtt ctaagaggcg aatttcacgt gccgagttgc gatttcgaag agtgccctgt
    37141 gtgcggcggc gaactccttt gttgtgattg catcttcgat gaaccggtgg aagatgcctg
    37201 atgaactcaa gcggctgcca cgactgggcc tttccggaat ttctacgaag cttgtgctac
    37261 ccgaggcatg aagccgcaca tggcgcgact cacgttggca cgcgagatgg ccgcgatcac
    37321 tttgctcgtc tggaagaaag gagttcgttt cgacgccgaa catttgaaac aacaagcagc
    37381 ttgagcgcct ggtcgagaac ccttccattc ccggattcat ttctggcgat ggctggtgcg
    37441 ttcttcccag gctctggttc gagggtgccg aggcgatgcg gaccgaaaaa actttcgcgc
    37501 taagtgggct cccggttgtc tcgagactgc aaggcaggag atgacatact ctccgacgca
    37561 aacccgctct ctcctgtgta cgttacccaa ctcaagagtc cggttaccgc gaagttgaaa
    37621 cacaacgcaa cgaatccggc attcagtccc atgatgggat cgtgcttgcc gagcatgaga
    37681 agaacggcaa tcaggacgcc agtcaccatt ccggcgaaga cgccggagat ggtcactcgc
    37741 tttgaataca ggcccaacac cgcacctgga aaaaactgcg ccaccccggc atatcccatc
    37801 agcagaagac ctacgagagt cgtggaacca aaaatggcag aaccaagtgc ggcgcacgtg
    37861 attgccaaga cggtgatctt cgccaacctc ccaacctgtt ggtcggtcat gccgggcgcc
    37921 agaatcgggc ggcacaggtt ttttgcaaac aagcttgccg ccgtaagtat taggatcccc
    37981 gccggaacca tcgccgtgag ggcaccggct ccgccaatca ccccgaggaa ccaggacggg
    38041 aaggtcttgc gcacaatcat taacaacgag aggtcgccat tggcgagccc aggcaacacc
    38101 agaagcgcgg ccaggcctac aaagaacatg agcggcatgg taatgatgta gaggggcatt
    38161 acgaccgcgt tccggcgcaa cgtgtcgcca ctttttgcag taaagctcgc gccgaagaag
    38221 tgcggccaca tataaaatcc aaatgcgcag agcaggacgg ttgatacata ccaggcgtgt
    38281 ccgagattcg tggtcgcccc aggcattacc aaatggcccg gttgggcgcg cgcgaccgcc
    38341 gcaaacatcg gaccaatgcc tccaaagtaa atgtatggca ctgcgacgcc aacaaaaacc
    38401 gcggcaaaga atagaacaag gtccttcagt gcgctgaccc acgcgactcc ccggatcccg
    38461 ctcgtgaata caaagccggc gaccatggcg aaggagatca ccatggccgg cgtgcgatga
    38521 atcactccga aactcgccac ttccacaatg attccaagac ctgtgatctg aagctgcaaa
    38581 taggggatga tgcagatcat gcccacaagg gcaacaaagg cggtgagata cttgctgttg
    38641 tagcgtactt gaaagaaatc ggcttgtgtt tggaggcgat atcgccttgc ggcttcccaa
    38701 agttcgggga gaatatagaa agaaaccaca taggctaaac aggtgtatcc gataatgtac
    38761 agaacgggtg cgcctctgga gtacgcccag ccactcgccc caaggaacgt gaacgtggta
    38821 tagatctccc cggccatcag caaccagacc agcaacaggc cgaaacctcg accgcccacg
    38881 atccattgtt cgagatccat cttgcggtga gatccgccaa gaaaaccgac aaacgcgccc
    38941 gctgcgacga tggcggaaat tacggtcaga gcaacgacgc ttgaactcac agtgtgccgc
    39001 cgtctttgcg atgtggctgt gaagcgcgat gtgtttccaa ccgatatgcg ccccacatac
    39061 agagcggagt gaggagcagc cataagatca accagaaaaa gttgaacggc aaacccagaa
    39121 ctgtgggata cacacggtcc caaagtgaca ctgaaaaaca tgtggccgcg aaaggaatta
    39181 gccccagaag aattgacgcc ggagatggac gcttcgcgga gaacctctga tcatcgcggt
    39241 gttccatgtg cgcgggagtg taacagcatt ttgcaagaca ggttggtcga gatggaaaat
    39301 cgctcgacgc gcctggcggc gcagtcggaa aggatccaga ggcctgatta acgcactgag
    39361 cggacaagta cccgctgcgc cgagaatgtg tgtttcccgc acgccttaaa tctaagaatt
    39421 ccacccgctg aaaacaacca cttggcaacg ctgtttcaag aacctttttg gcgcacgctg
    39481 gcacagcagc ctttcaccgt gagatttcag ggggagctat cttctcgaag taggcggcga
    39541 caaccgatct tgcaacgggc ttgcgatact cacgaaaatc gccattcgca cgacagactc
    39601 caggcagttc ctatccatct ggttgggcga tatttgcaca actccctccg agagtgcctg
    39661 atgcggtaaa tgggtaaaat gcttagggct ccgcccgaat aactaggacg ggacacggcg
    39721 cgtgatgcaa gacctcttgg gagacgctgc ctagaaaaag gccgtgtatt tttccacgcc
    39781 cgtgactgcc catcacaatg aggtcggcgg accagttctt cgccacctca atcatctttg
    39841 aaacgggttt gccagactcc aggaactcgg ggacgtcgga gctcacagcg cgcgctgccg
    39901 aaatgtcgca agaagttgct tcgcttctcg ttcagacatc tccatcaact atctgccggt
    39961 tcaccccaca tcgttccagg agcgaccgac ggttcaaaca cgtggacgaa accaacttcg
    40021 gcttgaagtg actttgcaag cttgctaccc acgtcagcag cgtgcgccgc aagcgcgctg
    40081 tcgaggcacg ccttgtcggc aatcactaac tcgaagccct ggcgctccat gcataccccc
    40141 gcgtctcggc gatgcaggag cgtcttcatc gcgggctaag aacagtttgt tataatcctc
    40201 cccgtcatga agcttcgatc gctcatgttc cttgactccg ccgcggccca aaggccgaga
    40261 gactgattct gaaaggccaa gccttccatg caagggctgg taaatacatc tcaagtgatg
    40321 gccgccctcg aacgggggat tgaaggagct ggtgtatccc gcgagcaggc gctgttgttg
    40381 atcgaggatg cccagctagg ttccttgctc gaggcggccg ctgcgatccg gggccgatcc
    40441 aagggccgaa cagtcagcta ctcaaagaag attttcatcc cgctgaccca cctttgccga
    40501 gattattgcg gctattgcac gttccgagcg gaccctgcgg tcgatggcga gccatacatg
    40561 acatcgcaag aagttcttgc ggtggccgaa gccggccgtc gtgcgggatg caaagaggcg
    40621 ctgttcagcc tgggtgatca accggaaacg atattcccgg aggcccgagc gtttctgcga
    40681 aagcagggct tcgagcggac gctcgaatat ctcgcgacga tgacgcagct ggtcctcgat
    40741 gaaacgggac tgttgcccca ctcgaatccg gggatcatgg acatagggga cctggaatgg
    40801 ctgaaggaat ccaacgtcag cttggggctg atgctcgaga gcacgagctc caggcttcgc
    40861 cgcccgcaag gtccgcactg gaaggccccg gataaagtgc catcgcttcg attgcaaacg
    40921 atcgaacggg cgggactgct atcgatcgct tttacaactg gtattctcat cggcatcggg
    40981 gaaaccgcag aggagcgtat cgattcattg cttgccattc gcgatctcca cgagaagtac
    41041 ggtcacatac aggaagtgat tatccaacca ttccgagcca aaccggggac gagaatggca
    41101 cgcgagcctg aaccgtccac ggaagacctg ctgcggaccc tggccatcgc gcgattgatc
    41161 tttggcagtg acatgaacat ccagtctccg cccaacctac tctcggacga ctacccgcgg
    41221 ttgctcggag ccggaatcaa cgattggggc ggaatctctc cgctgacgaa ggatttcatc
    41281 aatccggagg cagcatggcc acagatctct gtactggccg ataagacagc cgcggccggg
    41341 ttcgtcctgc gcgagcgact ggcgatttat ccggaattct cgtcgcggcc acgattcgtg
    41401 cgtgcacccc tccaatctgt tttgcagaag ctccagggag aagacggtta tgcccgaaac
    41461 tgagcgtggc aaatgctgaa cattcaggac cctcggtggc gagagatctc ctgggagcag
    41521 gctttcaagg ctgcgaatcc gacggttgcc gatgtcctcg agaaggctct cgctggaaga
    41581 gatctcgatg cgagcgacgg actcactctg tgcggtgcga cagatgacga ccttctcgcg
    41641 gtgctgaaga ttgcgaatga gcttcgccgg cgcgccgtcg gcgatcaagt gacctacgtc
    41701 gtgaaccgga acctgaactt cacgaatgtt tgcattgtag gctgcgcgtt ctgtggattc
    41761 ggtcgaggcg cccgctcgcc ggatgcctat tttcattcga ccgagtcgct cgttgccaaa
    41821 gcagtcgaag cggccgagcg cggcgccacc gaagtgtgca tccaaggtgg attgccccgc
    41881 gacttggagg gcacctatta cgctgatctt ctgcgcacca tcaaatcgca tttgccgaat
    41941 ctgcatctgc acgcatattc gcccatggaa atcgtgtacg gtgtcgaaaa gaccggcgtg
    42001 aatctgtctg agtacctgcg aactctgaaa gaggcggggt taggaagtat cccgggaaca
    42061 gcagccgaaa tcctcgatga ccaggtgcgc aaggccctga gcccgaacaa gctcaaggtc
    42121 caccagtgga tcaacgtgat tcgaacggcg cacgatctcg gcattccgtc cacgagcacc
    42181 atgatgtacg gtcacgcgga actgcccgag cattgggtgc ggcatttgct cttgctccgc
    42241 gagattcaga tggacacggg cgggttcacc gaatttgtcc ccttgggctt catccactca
    42301 cagaccagat tgttccgcgc aggccgcgcg cgtgccggga gttcgctcgc cgagcacttg
    42361 ttggtgcatg cgctcgcccg ccttcttttg aacggacaca ttagcaacat ccaggtgtcc
    42421 tgggtgaaac ttggtttcga aagggcattg gcctgcctgg aagccggcgc caacgacttc
    42481 ggcggcacgt tgatggaaga aagcatctca aaatccgcag gagcgaattt cggagaatct
    42541 gtcttagccg gtgagcttcg cgcgctgatc cggacgattg atcggacgcc cgccgagcgc
    42601 accacaacct acaagatccg tcgcgtgttc aaacacgcag atgcagaaga acttctgccg
    42661 ctggaacgca tggccgcgca ccgagaagat cccatgctgc gcgaccaggt gggatactaa
    42721 tggatatcgt ggcactcgcg ggcgggattg gtgcctcaaa gctcttactg ggactccatc
    42781 gcgtgatgga tccgcgcgac ctgacggtca tcgtcaatac gggcgacgac atcgtcctgc
    42841 acggcctctc gatatcaccc gacctggata ttgtcacgta cacgttggcg ggcctggtga
    42901 atcccgacac ggggtggggg ttccggggcg aaacgttccg cgcactggag cagctcgccg
    42961 tttatggtcg cccccggtgg ttccgtcttg gagatcgcga cctagccacc cacatccacc
    43021 gctcggacat gctccgcgcc ggggcgcgcc tcaccgaagt ggcggattcc atccgagaag
    43081 cgctgggtgt ccaggccaga attctcccca tgtgtgagga gccggtgcca accatcattg
    43141 ataccggtca gggacgcctt cactttcaag aatacctggt cgagcgacgc gcagagccga
    43201 tcgtgcgggc catctcgttc gatggaatcg aacgagcgca cccggcccca ggcgtcctcg
    43261 aagcgctcga aagtgcccgt agtatcgtga tttgcccgag taatccgctg atcagcattg
    43321 ggccgattct tgcagtcccg ggtgtgcgag atgccatgcg aaagcgccgg acccacgtcg
    43381 tcgccgtgtg tcccatcgta ggaggaaaat cgctgaaggg gccgtccgat cagatgctcg
    43441 cgcagctggg gcacgacgta tcggccctcg gagtggcgcg aatgtatcaa gacttctgcg
    43501 gcacgatggt gatcgatccg atcgacagcg cccagggtcc ggcaatcgaa tcgcttggtg
    43561 tagaggtcgt catccaccca acggtcatgc ggaacctcga ggacaaggag ctcctcgcgc
    43621 gtagaatcat ccagttcacc gcgaaacaga aggcaggagc cgctgcatga aggcgatcct
    43681 gattccggta aaggaatttc acgaggcgaa gaaacgcctg gctccgcatt tctcccctgc
    43741 cgaccgcgcg gccttggcgg aagcgatgtg cgaggatttc ttcagcgtcg tggcagcaac
    43801 gtgctgtgcg gaccgcattt tcgtgatcac caaggaaccc ggggctcttt cgcgtgcccg
    43861 agcgcgcggc tgggaaacga ttgtcgaatc tcaccagacc tcggaaagca aatccgtgga
    43921 tgcagggtcg cgctactgcg ccgagcaggg tgttcgcgct ttgcttcgac tgcctatcga
    43981 tcttccgctg gtcgaaccgc gcgacatcga ggcagtcttc gaacaattgg acgtggcgcc
    44041 gtcggtggtt atggttccct cgagtgacgg cacgggcacc aatgcgctcc tgcgatcgcc
    44101 cgccattctc ttcccgtcgt acttcggccc caatagtttc ccgcgtcact tggccgaggc
    44161 gaaggggtgc ggcgcgcgta ctcgagtttt tcgaaatcct cgtttagaac tcgatattga
    44221 tgagttggag gatctgcgga ggctctcgaa tcgactgcgg cccggttccg ccacggcgcg
    44281 atggctgatc gaacgtgacg ttgatctcgg agcgccgcgg gctagcgcag ccggcagtta
    44341 gatgacctga tctcgccgga aagcggcgat ttcagcggcg gtgtaaccga gttcacgaag
    44401 aatcggctcg gtatcctgtc ccaaggccgg aatgggatcg aagcgcgctg gactatcgga
    44461 cagccgtagc gggcttccca tgacgggcac gggccctaca ggcgattcca tttcccgaat
    44521 catttttcgt gcgatcgtct gaggatgagc caaaacctcg ccaataccgt ttagcttccc
    44581 gtgcggcaac tcggcctctc caagacggcg catccattcg tcgtgcggct gttcgagaaa
    44641 cagcttctcg accatctcct ccaacatcgc acgattctcg cgacggtctg gggcggtgcg
    44701 gaaccgtgcg tcttcgagca actcgggacg ccgtgcgacc ttccggcaaa atacttccca
    44761 atcctgcggc gtggccacgg cgaggctgac gtagacaccg tcgccagcca tataggggcc
    44821 gtagggaacg atgtagtggt gacgcatgcc gacacgcgca ggctcctcgc cgcgatgcca
    44881 gtagtgatgc ggaaaatatc cgagccaggc gagcgccgat tcgaacatcg agatgtcgat
    44941 cacttgtccg cggccggttc gttccctttg ataaagagcc aggagaattc ccaccgtcgc
    45001 gtacatcgac gacgcgaggt cggtgatcgg cactccaacc ttggccggct tatccggata
    45061 gcccgtcgtg gcaatgatcc cggcttcgcc ctgaatcagc aggtcatacg ctttgacatc
    45121 ccggtagggg ccgtcttggc cgtaaccgga cagagaacag tagacgagcc gtggattgcg
    45181 ggcgcagagt tccgcggctc ccaagcccaa tcgatcggta actcccggcg cgaaattttc
    45241 gaggacgaca tccacgcgtt cggccaatcg ctgtacggct tgccggcctg ctggcttctt
    45301 gatgtccacg gcgaagctgc gcttgttgcc attgagccac acatagccgg aggaaaggcc
    45361 gcgcacgacg gagtcccacg cgcgaatcag atcgccggtt cctggctttt cgagcttgat
    45421 gacttcggca cccatgtccg cgagaatccg cgagcagtgc ggacccgaca cggacacttc
    45481 cagcgccagg acgcgaacgc catccagagc cagaggcgcc tttttggcgg caggttctgc
    45541 catcaggggc ggacctcggg gaacagattc tgcttcggcg cgtgggcccg tttatagacc
    45601 ataacgctgc ggctgtagtc gatgacgacc ttgccatcct gctggatccc gcgagtgcgg
    45661 accttgacga tcccccactg gggttttgac ttggactcgc gcttctccaa cacttcggat
    45721 tcggagtaga gcgtgtctcc cgcaaacagc gggttgggta gcttgatttc gtcccacccg
    45781 agcgcaaagc cgttttcgct gacgtccgcc acgcccattc cggcgacgat ggccagcgtg
    45841 agcgcgctat tgatcaggca tttgccgaat tcggtgcgtt taccgtattc gttgttgaag
    45901 tgaatctgat tcgtgttgtt ggtcagcagc gtgaaccaga tgttgtcgtt ctcggtcacc
    45961 gtccgcccga ggcggcagcg gtaaacgtct cccacttcga aatcttcaaa gaatcgcccc
    46021 tcccaaccag gcttgactgc cattttgatt cctccccgat cgatttctaa attgaactaa
    46081 cgtgggaatg aaatgtttaa gccccacaac tcctattggc gtcgtagcct cgttctgcgc
    46141 cactacggcg agagccaaac ccactacgag gaacgagtgc ccagcgattc cgccagctca
    46201 agagtttgcc gggccctgtc cacaattgga agatcaagga gttgcccgcc gaaggccaca
    46261 gcgccctcgc cgcgggtcct cgcctcatcg aaggccttca gcactcttcg cgcatagtcg
    46321 atttcgtccg ctgccggtgt gaacgccccg ttcacgattt caatctgcgc gggatgaatg
    46381 agtgacatcc ctgagaatcc aagacggcga gactggaggg aaaatgtctt cagaccttcg
    46441 gtgtcctgaa ggtctggcca cacgccgtcc acggattgga cgtgcgcgca tgccgcggcc
    46501 gtcacaagcg aggaacgagc atagatgagg tcacgagctt cgccttcacg ccgcaacggc
    46561 aggctcagct cgcgcgaaaa atcctccgcg ccaaagagca gtccgatgac tcgtggcgaa
    46621 gctgtcgcga tcgcgtatgc gttatacagt ccttttggac tctccaaagc gcacagcagc
    46681 cgcacgcttc ccttctgcag ccctacttcg ggttcacggc gatcaagaac ctgagcgacg
    46741 ctttggatct gctcaggagt ctcgaccttc ggcacgacga gcccttcgag ccccttgcgc
    46801 acaacggcgg caagatcgtc gcggctgcgc tcgtgcccga cggcgttgat gcgcacgtag
    46861 cgcgcgggcg tgcgcagttt cggctctgcc ttcagtcgcg ccgccacttg gtcgaggcag
    46921 gtggcgatct gctgacgcgc actgtccttc tccgccggcg ccacgccatc ttcgatgtcc
    46981 atcatgatgg cgtccacggc gacgcccagc gatttctcaa tcatgcgctg acgattcccg
    47041 gggacgaaca tccaggatcg cattagctca tttttccggt ccatggtttt gcaccacccc
    47101 tctccattga tgaattctga atcgattgct tgcaggcttg cgcctgccgc actccggctt
    47161 cttgccttcc ctctccttta atacgacttt ggcatcttga gcacattttg gccgagaaac
    47221 gccagaatca aattgttgct caccggcgcg acgctgagaa ggcgggtctc tcgaaacttt
    47281 ctctcaatat cgtaatcgcg tgcaaaaccg taaccgccgt aagtggttag gcatacatcg
    47341 gcagcttccc atgccgcctc ggacgctagc agcttggcca ggttggcctc agcgccgcat
    47401 ttctctttgc ggtcgaactt cgcagcagca tcgaagcgca tgagatcggc agcctccacg
    47461 tgggcgtaag ccttcgcgat ggggaattgg acaccctgat tcgtgccaat gggctttcca
    47521 aaaacgactc tttcacgtgc gtacgtcgat gcccggtcga tgaaccagcg gccgtctccg
    47581 attgcctcgg aggccaccag aatccgctcc gcattccacc cgtcgatgat gtaccgaaac
    47641 cccttgccct cttcgccgat gcggttctcc accgggacct ccacatcctc gaggaagatc
    47701 gtattcgtgt gatgattcac catcatgtcg agggggcgga tttgcatgtt ccccttgacg
    47761 ttccgcatgt ccacgatgaa cacggaaatc ccctgcgttt tgtcctgcac gtcgctgtac
    47821 ggtgtggtgc gagccaacag cagcatcaag tccgattgtt tcgcgcggga gatgaatatc
    47881 ttctgcccgt tgacgacgta acgatcccca tcgcgaacgg cgcgggtttc gatgcgggtg
    47941 gtttccgatc cggcattcgg ctcggtgacg gcaaacgctt ggaggcgcaa ctctccccgc
    48001 gcgattgcgg gcagatagcg ctgcttctgc tcctcgctcc catgacgaag caaggtcccc
    48061 atcgtgtaca tctgggcatg gcaggcgccc gagttgcctc ccgaacggtt gatctcctcc
    48121 aggatgacgc cggcttcagc gatcccgagt cccgcgccgc caaattgttc cggaatcaat
    48181 gcggccagat acccgctggc ggtcaatgcg gtgacaaagt cttgcggata cgcttccttc
    48241 ttgtcgacct cgcgccaata cgtgtcgggg aagtccttgc aaatcgcggc tacattctct
    48301 cggattgcac gtagttcctc cgtcgtcgtc cccatgtcgt gtcctccgtg ctcgggaacc
    48361 cgtattgcgg gcctagtcaa gccacatcag tttggccggg ttgtcctgga gcatctgata
    48421 gaaatagtcc ttggcgttga gctggcgagc acgctccaca gtcaacaccc gccgccccac
    48481 cagaagctgc cgggcggcct ctgcgtcagc agcaaaatcg cacatgatcg tctcctggta
    48541 agacttggtc tacgaattcc cccacgcttc tacgaatcgc cgggcatcct cgatcactgg
    48601 ctcggtcaca ggaacgtacg gaacaatcaa ccgtgtgacg cccgcctcca caaacggttt
    48661 caaccgatcc ttgatttcct ggatggaggt cgcgggaatc accggcaatt tgtccatgta
    48721 agcatccgtg atcttggtgc tggcagcttt gaatccgccc gccttgaagg cttcctggac
    48781 ggcgttcacc tcttcctcga atcccgatgc cctgagcatg tccttgtagt ggtcggcgat
    48841 gttgtagaag gtcagcacct gccggatgcg cgacctggcc atggctcggt ccggatgtag
    48901 cgaaacgcga actttcgcga tgatgtcaat cttgcttgga tcgcgtccca cctcaacggc
    48961 gcccgcacgg acgcggtccg cgatctcgcg aatcttggcc ggcgtcgcca tattgataaa
    49021 gactccgtca gaaatctgcc cgaccagccg cgtcatctgc ggtcccaatc ccgcaaggta
    49081 aatcggaaag gttggatgag acggtttcca ggaaagctgg aagcgctgtc cggtcgaata
    49141 catctcccct tggtattcca cgcgttcgcc ggcagcggtc ttccgaacga tttcgatgta
    49201 ttccttggca cggcgcaggg ggcgatcaaa cgtgcctccg tgccaagcgg cgatcgtttt
    49261 gttagccact cccaggccca acagtagcct tcctccggat agatcctgca aggtggccga
    49321 ttgaattcct agagtcaccg cactgcgtcc gtagatgtga tagatcgcgg tcccgacttt
    49381 cagtgtgcgc gtgcgcgcag cgatgcccga aatcaggacc agaggatccg tgctgttcga
    49441 ttcccctttc cagaaggcgc cgaaaccggc ttgttcgatc aagggggata attccacgat
    49501 ccccgcgacg ggataatgtg cggcagagtt caactcaatg tcataaagca cggtcgctcc
    49561 tcgctttccc cgcaactgcg cacagagaac ggctctcgct cacctgtgat acacctcccg
    49621 ttcgggtcaa tttcctcccg gaaccggcgc agcgcttcgg cagcgggaag tgtcgtggtt
    49681 ggcacgtctt tcgggatgtg caacggaaac cctgtatcct ctttcaactc ttaaagtccg
    49741 atccccggat gcaatggaac cacacaaaca gagtgcgtct cagcgtcgaa ccccagcaca
    49801 gccatgtcgg cgatggtgcg gttcggccct ccatcccgca ggcccaactt ctcccggcta
    49861 cgtccttcca cgaagcccgg actggtcagg tgggctaccg caagcggagg ttttcgtttt
    49921 tatggcgggt gtgaggcccg caacggacga gaggttacct atttggagga ccataagtca
    49981 agaaataatt gtcccagtta tcgcattgcg agaatatcga cctgttcagt tgactccgca
    50041 tgccagaatc tgatactcat gtcacatgtg ttgatgcaga tgtttgacgc tcggaagtgc
    50101 cttgacccct tggtcggcag ctagcactct ggctgctgtt tcgctggcca ggcgtgccag
    50161 ctgtaaccgc gcacagagaa tctcggaatg gcacgcgaga gagaggaaca cgtgaataca
    50221 gcgagtcgat tgctggcggt cctaaacctc ttcacgattg aaaagtctga atggaccgtt
    50281 gaggaggcag ccaaagaaat tggcgtctcc gtgagcacgg cgtacagata cttccgagac
    50341 ttgtctaagg tgggccttct tgactcgtct cccagcgcca aatatgtact ggggcccgca
    50401 gtcattgaga atgatcgcaa gattaggcta accgatccgc tgattaaggt cgggcggcct
    50461 gccatgcagc gtctcgtcgc tcgctcgggc agcgcgggcg tggctctgtt gtgccgtatc
    50521 tatcggaatc gcgtcatgtg cgtgcatcaa gaggccagag ttcttcctga aaataccgtc
    50581 ggctatgagc gcggccgacc gatgccgatg tttcgcggcg catcatccaa ggtcattttt
    50641 gcgaaccttc cgtcgagaac agcgcgatgg tttttcgaga agtacgctga cgaaatcact
    50701 gcaggagggc tgggctccga ctgggaaacg atcaagctca atctgcgccg catccggaga
    50761 tgtggcgttt atattacccg cggcgaagtt gacagcggcc gcgttggtat agcggcacct
    50821 gtatttggcc cagacaggag cgtgatcggt agtatcacca tggccattcc agacggtgag
    50881 gcgactcccc agttcgtggc caacatctct gcgcttgtgc aggcagccgc tcgcgagatc
    50941 gacgcgggtc tgcagttttt gtcatcgggt gagctcgttc aagatcctgg ccagccgccc
    51001 gtttatcacg gactgtccag ctctcaagat cctggcattg ccgacacgtc gacaggctct
    51061 tgattttggg atcgggcgga ttacctcggt actggattgc ggtgcgagat gaaacggttt
    51121 cgacagacat ccagccgtcc ctcatggcgg tcgggcccag agggtttgat tgatcttcgc
    51181 ggtattgggg cttggctgat atgacctctt ggggatgtaa accgctaccg aaacaagtct
    51241 ctctccgccg gccgaataag gtcgcgtccg cgtccgcttc cctgcgatat cgccaaaccc
    51301 tggagaaccg ctgccgggac gcggttgatc ttgcccatga taagtccgcc agcagccgcg
    51361 agttcatcgg ccaacgcaat cacagtcgct tgaagtgtct ttccgtgcgt gtcgcgttgc
    51421 ccccgataat cgaccaatgg tgccattccc gccacaccca gcgcgacgtt caccaagcct
    51481 tcccgccacg ggcgtccaaa cgtatcggaa atgatgacag ccacatgaac gccaaaagct
    51541 cgcgcgagtt gctcctgaag cgtgcgtgcg gagcgatccg gatcctctgg cagcagcagc
    51601 gccgttccac ttggaacgtt cgagacatcc acgccggcat tggcacagac aaaaccgtgc
    51661 cgcgtcttgg cgatgatcac gccgcgctcc atccgcacga tgctgctcgc ttcccgcaac
    51721 acaatttcga tcacgcgagg gtccttttgg tattccgcgg cccactgttc cgcttgctcg
    51781 gaaggtttga cggactcgag actgacgatt cttccttccg ccttggacac aaccttttgt
    51841 gccacgacga ggacgtctcc ttgcttgata acgagtcgtt gccgttgggc agcttccaca
    51901 aggagccgtg caagatcgtc accacacgcc acctccggca gtcccgctaa cggaataatc
    51961 tgcaccaggg agggagatgg tttcttgagt gtctcagtat ccggcatgtt tgctgaattt
    52021 agacagcgca cagactagca tccggagcgg aacgcaccaa gcggttcagg ggtgggctgc
    52081 gggcttctct cgcggggacc gcgattgaat ccgggcgcgc tgatcagccg cacgacaagt
    52141 gggcgcgcgg agcgatcgaa aagtgggtcg tgtctcgatg gaaagcttcg aattgggtct
    52201 cacgtccaga ggtatttcgc ggaggaaata tgagtccaga gaaaattgcc attatcggag
    52261 gaaccggtga cctaggattc ggcctcgcct tgcgatgggc acgggcgggg ctgcaggtca
    52321 tgatcggctc ccgcgatgaa accaaggcca aggaagctgc gcagcgcata cgggacgcgt
    52381 tggacacaca cgccccaact gagtcggggc gcattttgat cagcgggctc gagaacgcac
    52441 aggccaccgc acaggcttct gtcttagtgt tggccgttcc gatctcggcc caggtcgaca
    52501 tcctaaagag tattcgaggc tccctcggag gtgccatctt ggtggacgct accgttgcct
    52561 tggccgcagc cttgggcggt aaaccaacgc ggaccctcag actttggcaa ggatccgccg
    52621 ctgcacaggc tcgcgaactt gttccagcca caacgcctgt gctggcagcc ttccacaatg
    52681 tctcggcgga tgctttgcgc gacctgtcgg taacgcccga gtgcgacatc ctggtctgcg
    52741 gagatgatgc ggcagcgaaa caaattctct ttcggttggt gaacttaatc cctggcttgc
    52801 gcccgattga tgcgggcccg ttggaaatgt ccagcattgt cgagagcatc acagctcttt
    52861 tgatttcggt gaaccgccgc tatcgcgtcc atcacagcgg cattcgcatt acgggattgg
    52921 agctcaaaac atgatagagg taacgagtca aagcggggct gtcaccaaaa accatcagca
    52981 cttacattgg tttggtcaaa tttgtcgtgg cgtccgcgct ggacaagagc ggcgagcaaa
    53041 tgtttccgcg caagtggaat cacgagttca tcgatctgcc cgttgtcgga catcaaccct
    53101 cgtttgattt cgacacaatg actaacattg tccacaaagc aaacgggcag gacagagttc
    53161 tccacgcctt gcttgcgggg accggactca gggcaggcga agctcttggt ttggaattga
    53221 aacacctttc ctcagattgt cagacgatcg cgattgagca aagttgttag gaaggtgata
    53281 ttcaaactcc caagacgaag aatgcttatc ggcaagtgga cctctgcccg atgctggcgg
    53341 aactcctcag aactttcgta aaggatcgtt agtctgggtt gctgtttgcg aacaagccgg
    53401 gtaagccgtt gtcacaatcc aatttgcttc gccgctctct ccatcgaacc ctcacggaac
    53461 ttggtcgaga aaaggcgggc atccatgcaa tgcgtggatt cggtgccaca tggttgagga
    53521 agcagcgaac tcccgaagac ccaatccgat tctggctcgg tcatgccaat cagtctgtaa
    53581 cggatggctg ctccaagctg gcggataatg tggagtttcg caagcaggtt gctgagaaaa
    53641 tcgggtcggg atttgacgtt ccagcttacg agaaaactgt attggtacct atgagagcta
    53701 gaaaacaaaa gaaaattcaa aaggcagctg cggcttaact atcaaccact gcgcgggtta
    53761 gagtggcgcg cccggagaga ttcgaactcc cgaccctctg gttcgaagcc agatgctcta
    53821 tccagctgag ctacgggcgc gtgaggtcat tctaacgcga attgccgatg gtccgcacgc
    53881 gacagcattt tcggagatgc ggagggcaca ttcagtggga tcggcagcaa agttgtatcg
    53941 ctaagtcatt cgtcgagggt cgcagctgaa tgcgcgaacc aacactagcg gtgaacgctg
    54001 gttgccttga tcttcacggc acctgcgcca cgcagacgat tttcgatctc ggcgcgtgag
    54061 gcatcggtcg gatccaaaac tccgatcaga atgcgtccgt tggagacctc gggatcgtag
    54121 agtttgggtt tccaattcgg cagtttcgtc gtgatgagaa gcgtgacgac cgtagtgagt
    54181 accgcgccga gcatcgtcag ctcgtaggtg acaatgcctg aaggccaggc tgcgaaaatc
    54241 ggcattccac cagtgacgat cggatacgcg acctgggtca gccgcgcaag aaggaatccg
    54301 cacatacctc cgaccaagcc ccctgctgcg gcgatccaag gcatgggcgt ctcgtggtcc
    54361 attttgctga acgaatagtc gtcgaacggc tcggcggaca tgaccacgat tttctttggg
    54421 ctgatctccg cgcgatgcag cgcgttcatt ccgcgctcgg cagattcggg atcgacaaac
    54481 aatccgtaaa tgactggcgg acttggcata tcagtctgcc cccgattcgc tggaccgaac
    54541 gacaaggcgc acggcgtgag ttttcacgtg ctggccggct ttgagttccc aaatggagat
    54601 gatcggaacg atcttcgaaa aaatcatgta gagcaagacc atggcgcaga aggtgccggc
    54661 ggtaatcgtg atttcaaccc atgtcgggcg atacgtgccc cagctatagg tcaaatattt
    54721 gtgggatagc gacggcacga tgattaaaaa gcgctcaagc cacattccga tgaccaccgt
    54781 gaaggacgcg atcgctgtgc ctgtaatcgt ccggagcttc ttgattgcga ggatcggaaa
    54841 tgggatcagg aaattgcagg ccaccatggt ccagaagagc ggcgagtagg atccgtgttg
    54901 ggtggtccaa aataccgcca tctcggacga ttcgttgcca taccaggaag tgaggcgctc
    54961 ggcaaacacg aaatagaacc acaggaggct catcaccagc aacagtttgc cgagattctc
    55021 gaaatggatc ggcagcagat attcctcgag gtggagaaac tttcgcaaga gcgccatcgc
    55081 gagaatcagg gcagcaatgc cgctgaaaat cgcgccggcg acgaaatagg gcgcgaagat
    55141 cgtcgagtgc cacatcggca ccggcgccat cgcgaagtcc caggacacga tgctatgcac
    55201 tgaaacagcc acggggatca cggcgatcgc catgatatgc atggcggttt cgaggcggtg
    55261 ccattgcttc ggtgtgccgg tccagccaag agcgaccaca ccataaattc tcttcttcaa
    55321 tccttcggaa cggtcgcgga ccaaagcgaa atcgggaacc atgggaagca gaaggaaggt
    55381 gacgctgctc atcagatagg tgttgatggc gaaaaagtcc cagagcagtg gcgaacgaat
    55441 gttgggccag agaccgcgct cgctcggata cgggaagagc cagaaggcaa gccacggtcg
    55501 cccgaggtga atgatcggaa acgtcgcgcc gatcgagagt gcgaaaacgg tgatgacctc
    55561 ggcgcagcgc gttacgggcc ggcgccaagt tgcgttcgcg aggcgtagga tggctgagat
    55621 cagcgtaccg gcgtggctga gtccaatcca gaagacgaaa ttcgtgatgt agaagcccca
    55681 gaaaacgggc gtgcgcaatc cggatacgcc cagccctgcg tacacttgat agaaaaacgc
    55741 gcacagcgcg gcgacgacaa cgcccccgaa aaacgtggct gcaagcagat agcgccccga
    55801 cagcccagag agggggcgca gaaggtcgac ggtaatttgc tcagccgatt tcgttgccat
    55861 gccatgaatc ccgtgggagg taagtaactt tgggccgtgt gccgacttct tcaaggagct
    55921 tcgttgcgcg gcccgaatgc gccaggcgcg acacctcact ttccggatca ctcaagtcgc
    55981 cgaataccat tgcacgcgtg gggcaggact gcacgcaggc cggctgaatc tcgccgtctt
    56041 tcaagttccg cttttcagct tcggccgcga tttctcccgc cttaatgcgt tgaacgcaga
    56101 aggtgcattt ttccatcaca ccgacttcgc gaacggaaac gtccggattg agctgcaaat
    56161 tgagcggctt ttcccaaacc ggattgaaga aattgaagta gcgcacaacg taggggcacg
    56221 cgttcgcgca atagcgagtg ccgatgcagc ggttgtagac ctgcccgttc aggccttccg
    56281 atgtgtgata gctcgcgtac gtcggacaga ccggctcgca cggtgccgcg tcgcactgct
    56341 ggcagaaaac cggcctgaac ttcaccgtga catcaggaaa ttcgccctcg taatatcgct
    56401 cgacgcgaat ccagtgcttg gcgcggctgt gcgcagcctg ctgttcgcct accgtcgaaa
    56461 tattgttttc ggcgtggcac gctacgacgc aggcctcgca gcccgtgcag cgatccagat
    56521 caatcgccat gccccactga tgagccatat cctcgtacct tcctaacggt gcttgagctc
    56581 cggcgaagct tcggtcaagc tgccgccgaa caaaatcagt ttccccctgc ccacgttcgt
    56641 aagcttcacg cgcgtcgcgg cccaggcgag cgaaccggtg ccctcaaccg ccataggcga
    56701 aaggatcgaa atcggattcg cgccgcggcc gctcgcgtaa cgcgtaaagt tttcgtggcc
    56761 ctgcccgaca ggcattgcaa gcacgtcggg cgcaattccg ggagcgatca gcgcgggagc
    56821 ttgcaacttg ccgtgctgag acgcgacttc gaccaaatcg ccttgtttga tcccgagttt
    56881 ttccgctgtg cgaggattga tctcgaccca ggtgccccac atcgtggtcg aaagcggatc
    56941 gggcgcttcc tgcatccatg gcaggtgcgc gaccgagccg tcatagaaga gctgcgatgc
    57001 aaatggcagg aaatgaaacg cgtactcgct gggatcaccg tcgaatttcg gtgcaactgt
    57061 tctcggagcc gaaatgccgg atgctccacc ggtcggcgca gctttggctt cttccgcgct
    57121 ccaccagccg cccgattcct ttgctcttat ccagaattca tccgcgtctt tcaccgcctt
    57181 gcttcccttt tctttgtaga gcgcagtgaa cgacgcctga atcgcctcgt cgtaggtttt
    57241 ccacgggagc aacttcgctg aatcgccgcc gagctgatgc gcgacgtcta gcagtacgtc
    57301 cggcatcgag cgcgtgttat gcagcgggtt catcgcgggc ggcgcgacac ttacgaccgc
    57361 tttcgccgaa cctgatgacg gcacgtcgtc gagccacgac tcgagcggtg aatgatcggg
    57421 caagatcaaa tctgccagcg cgcttgaatc gtcgatgaaa ctcccaaagc tgacgatgaa
    57481 cgggattttg tcgagcgcgg cgcgcacgcc ccaggccgcg ggcgctgcga aaaccgggtt
    57541 cgcgttgtag agcaagagca ctttgacggc agccgccccg gatgtgatct gcgaggcgag
    57601 cgtctgaagc gaaacggccg attcctttgg actcggagca ggagcgaacg aaatgccacc
    57661 ggatttgccg acactgccaa gcagtgcatt gagcgcgttg accgcaagcg cgttgaaaag
    57721 accgttagac tgcgccacag ccgagtcgcc gattagcgca actgcaggat cgtgcgccgc
    57781 agcttctcgc gcaatgcgcg cgattctttc agccttgatg ccagtctgcg cggcgatttt
    57841 ctcgggcgcg tagtccggca atccctgcga ccagccttca atcagcgagc cggcatgacc
    57901 ggccgcgctc gcgggccgca gcttttcggc aagaaccaca tgcgcgagac cgagcgcgag
    57961 gatgccctcg gcgcctagct gcacgggaat ccattcgtcc gcgctcgcac cggtctgcga
    58021 aatgcgcggt tcgaattgaa cgaacttgcc gcgctcgcca ggacggccct tccgcatgtt
    58081 tccgaaccca agcgactgcg cgaccggcga gttccacgtg cccaagaaat ccgcgccgaa
    58141 agaaattagg tagttggatt ggccgagatc aacagttgga agctgcgcat ggccaaaact
    58201 tgcggagttc gcggcgcgga tgacagcgtc gtcaaagaaa gcgaactcaa caggtatcgc
    58261 tttcttgaag gacgctgtga agccatcgac aatggttttt cgcttgccgc gaagcagaga
    58321 cgtaacaaac gccagcccgc cttccgattt gctggcctgc aaatcggaga gctgagatac
    58381 aagctgcttg atcgcgtcgt cccagctgac ttctcgaaat tgtcccgagc ctcgcgcgcc
    58441 attgcgtgca agcggagctt tgatccggtc gggatgatac gtgacctgca gccctgcctg
    58501 gccgcgggga cagagcttgc cctgattgat cgggtgcgcg ggattacctt cgagcttctt
    58561 cgcaaggccc attttcagca ggccgagctg cccattgcgg atgacttcgg cttctccttg
    58621 catcacgcgc acgagcagac cgcaaccggc tggacagagc gtgcagatgc tcggcttcca
    58681 cgccgcgatg ccgggaatca aatcctcttc cggcacgaac cggattagct gatgatccgg
    58741 attgccgcac ccttccagcg ccgccgtcgc tccagccact gcggaaattt tcaagaagtc
    58801 acgacgttcc atgattttct attaccggcc ttcgttcatc gccaacacca cgactagtaa
    58861 tgacacgtaa cgcaatccac cgaaatgttt ttctgccggt ggcaattggt gcaaaagccc
    58921 atcgtgtgat ccactttgcg ctcggcgacc gtctgctgcc gcatgtcgcc gtggcaagcc
    58981 gagcaatcga cgcccgcgcg gatgtgcggc gcatgattga acttcacatg agccgagggt
    59041 tcgaatccgt agactcgctc ccagggaatg tcctcgccgc gcgcccgata tgccgcaagt
    59101 ttcctgatct cgggcttgtc cgtagcgatc acttgatgac aggtcataca gaaattgacg
    59161 ctcggaattc gcgcatccgg cccctgatcc acgccgacat ggcaatcagt gcactgcatt
    59221 ccgttgccga ggtggacttt atgggtgtag gcgatcggct ggacgggctt cgtacgtaga
    59281 cccagaaact cacgaatcga ctcggaaacg gttgaatgcg ccggagtgaa aatgtcggaa
    59341 gattgccccg aaggcgacgc gccgccctgc gccgacgaaa tcttcaccaa aatcgccgcg
    59401 aagaccgcga gcacaccgat cactgctgaa attcgaagat aattgctcaa acggaccacg
    59461 ggtcgcaaca aaagaccctc ttgattatta gaagtatgtc gccactacaa acgcggtatc
    59521 gtactctgac gcacttcaag tgtaaacgca atttcttcct tctctgtcat tggtgtcaca
    59581 cttgcgtccg ccgggaatct ctgcggaaaa ttccgtacgc gaccgcacag gtgagctgca
    59641 cgagaacgat caatgcggat ttgcgggaac ttgggaggag aaatgacgac gggaggcgcg
    59701 gaagcgcccc ccgtcgtttc taaagcgcta aggcaggacg taacggaacg aagtgaatac
    59761 catattctcg ttcgcgtgcg gcgcgccgtt ggtgctgtac gcaatgccgt cggcacccgt
    59821 tccgctcgca acgcctctcc atgtgttgcg gacgtagctg gagtactgca tgccgtactg
    59881 cactgttccc ttcggtcctt tgtagaagcg ataccagaac tgaatcgaac cctctacgag
    59941 gttgcgcgta tcgcccgtgc agttctgcaa cgcccccggg ataaaaccgt tgccgccggc
    60001 cacgctagtt gggacaccgg tactggccga agttgactgc gccgcaaacg gctgaatctc
    60061 cgcgctgcag ccccagttcg cgaagccctg cgcgccatag ccgacattgg gagtcgcgcc
    60121 agacttgatg tagcctgctc gtgccgaata ctcgccgcct acgtaaaggt tgatatcgag
    60181 cttcggcgtt gggtgcagtt gcaccgtgcc gagggcttgg tagttatgga gcagtgcgag
    60241 cgtcccgtcc accgcgggat tgaccggggt ggtccaacga atcgtcgcat ccggcagacc
    60301 aactgagcca tagcgcccaa tgccggtgcc gccgaggaag tggacgccaa gatcaacgtg
    60361 cttctcaaac aacgcccacc gcgcgttgcc gccgacgccg ccgccagtct tcgagtcgtt
    60421 gaatgcgccc gctccagacc aggtatctgc caccgaccct ccggaaacag ggagggtgcc
    60481 gcagccgggc actggggcgg ctgtggacgt tacggcgcac gggaaaacgc gatctcggaa
    60541 ctgagcaaat acgccgaaga tctcgtagtg tccaaatccc ggctcaaaca cggccttaac
    60601 gatgaaatcc ggcgacggat tgtactgata gttaccgagc ggattgtaga gtccaccgct
    60661 cgctccgaaa gcacccaaca ggaagtcgtt ggagacattc ggcagcagaa cggtgttgcc
    60721 actgacggcg ccagccgcca cattcgtgac gacgcgcgta ccgttaagtg ccgacgccga
    60781 gcacgtaccc gttgtggtag gcgttccggc aaacgggttg gcaggagcgc agctgaccgt
    60841 cggatttccg tgaaccgtga gggtcgcctg tggttcttcg acagagacgc ccaaccatac
    60901 tttgttgttg aaattcttgg tcacgcggaa accgaattgc cgagcccaac tgaagcccgc
    60961 cacgtactgc gcatcgatga cttggggcaa ggcttccgat cggttgtcca tgcccttggt
    61021 ggtttcagtg agcaagctcc actgctggcc gcctgtaagc gtccagccac tgttaaacgc
    61081 cgcctggccc cagaactgcc gttggcggag cgtatagctg ttgctttggt tgttgttcga
    61141 tgtggtgcca gcagagagga agtcggcttc atagtagccg ccaatcttca ctttgtcgag
    61201 tttgccttca accaacatgc cgaaccgcga ctgacgaccg ctcgcctgaa actccgacat
    61261 gtggccgttg gatgagccgt taaacggcac cgagttcaag ggcgtattga cgtcggccgc
    61321 gagcgccttc tgccgccaca ctgtttctgc cgccatgaag ccgatcgggg ttagagaaat
    61381 tcccttgatg ttgatggtgt tgggcgtctc cggcttcttg tcatcagcgg ctccggcatt
    61441 cacagccttg gtcgccaagg cgaccttggg accgatgctc tctggatcgg caggatcggc
    61501 ggtcacggct tccttcgccg ccgttgcagc ggtcagctgc tcctcgagca gctgcatctt
    61561 ctgctgctgt tcctgaatct gtcgggactg cgactccaag agatcgcgga gttcctgtaa
    61621 ttcactttcc agctccgagc tggaaggctc cgccttggcc gcggcgttcg ctttcggatc
    61681 ggccagcgac gtgttgcccg gctgagctgg cgcggcgccg ctcttcgcct ccgtcgttga
    61741 cggagttccg ttggcacccg tcccgcctgc cttcgccaga accggcgacg cagccaagcc
    61801 aagcgccaca atgcacgtag caagtgctcg cattccttcc tccttgaatt taactgcgaa
    61861 tctcccagca actacccgtt ttcaaaaaaa ggctttagat gaggccgtag gaacctagac
    61921 gccccgctcg tccccaggat cgatgcaaaa ctgggttgcg gggggaccca cccttgcaga
    61981 gccgccgggc gcggctacaa cgtaactctg cttccggata cacccgcgaa cgacaggcac
    62041 gttaagtggt gcgtgtgaac cttcggtgat tggattgtta attctgtgtt actgtcggcc
    62101 cgattctcgc gcgaaagcac gacgcgaacc tcgccgacac gcgctgcgtg gaagtagttg
    62161 acaaagtcta taaaataggt agactttatc gagtcactag ggtataagaa tagcagccat
    62221 gcgaatttca caacgagggc tttacgcgct gcaagcgctg acggttcttg cacagcatcc
    62281 gtccggcgtc gtcaagatca aggatattgc tgccgaggag caattgccgg agaagttcct
    62341 cgaggccatt ctcttggatc tcaaacgcgc gcgaatcgtc acgagtttgc gcggcgccaa
    62401 cggcggctac cagctcaaac ggccggcgaa gaaaatcgcg cttggggaag tcattcgccg
    62461 aatcgatggt ccgcttgcgc cgttcgaaga cgccgagtcg ctcagatggc gcatcagttc
    62521 cgatcccaaa catcgtgcgt tgttccgcat tctcctggat gttcgaaacg ctaccgcccg
    62581 catcctggat cacacctccc tcgctgattt gtgttgtagc cgcaactagt ttctcgccgc
    62641 agtccgctca gtaacgaaaa gcaacaagcc gtaggtgctt cacccataag tagtgcattg
    62701 cggagctcga aagttccgcg actcgtttcg agctagttcc gacggaggtg agcgaatgag
    62761 cgcaattgca acgcaggact ggcaacagga cttgtccgtg accgagcgcg aacttttgcg
    62821 cgaagtcgcg ctcgcgctga gaaccatccg ttacggctcg gtgacgctga ccgtccatga
    62881 cgggcgcatc gttgaattga acaaaacaga gcgggttcgc aaggctccgg catagctgcc
    62941 tac
//
LOCUS       DASPUL010000113         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1661846, whole genome shotgun sequence.
ACCESSION   DASPUL010000113 DASPUL010000000
VERSION     DASPUL010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661846"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1651
                     /locus_tag="VEJ00_02900"
     CDS             <1..1651
                     /locus_tag="VEJ00_02900"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HXX00135.1"
                     /translation="GSLILRVRHVDDDDSYEVDTPNIAFTLRQPGEYRLDVSADGTQT
                     IATVFHGRGRVTGGGFSYTVIANQSATFTGNDQHLDYDLAQLPQQDDFDSWAFDRDQR
                     EDQSDSANYVSREMTGYEDLDANGTWSYAAGYGEVWAPTAVAVGWAPYRFGHWIWVSP
                     WGWTWVEDEPWGFAPFHYGRWAFLGARWCWVPGPVVVRPVWAPALVAFVGGGPGFHFS
                     AGVGVGWFPLGPGEVFVPGYHVSRGYVNNVNITNTTVNVTKVTNVYNTVIVNKTTTIN
                     NITYVNQHVNNAVTVVSHDAFVNSRPVAQNVMKVEPHEIAAAPVSHVVAAEPVRTSVM
                     GAGRPVAVKPPAPVINRPVVAVRTPPPLPHPLDQRQTEAGGHLNQQLLVRPAGPARPA
                     QMTPATQQPAAQGGFKSFGQPSQPSSGNAQVKQMPQTQPRVYEQQGTPQPEPRPENRN
                     VEEQTNRNVQPENRNLQPQNRNMQPENQSRSFQPPPQEMTHPLVHPAPPVEEHNPQVE
                     QQKEQKFQQWQQQRPPASQPKSQPQPRSEPPKQSAPPKKGR"
     gene            1827..>2832
                     /locus_tag="VEJ00_02905"
     CDS             1827..>2832
                     /locus_tag="VEJ00_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXX00136.1"
                     /translation="MTDPKRSTINWMDGLWLMFLAGLALLPPVEEPHKQAILLAFGVL
                     QLAESWLVTRLPRRGPAYVVLLKIALATLLIDHTNEIAINSSYWPIYFLPAVTAAEYF
                     GPLATLLWTTLASAAYCSYLYQASFEFELTEEAYGLLAIRVLFFFLAAMVVNRYVVES
                     RKQTQRYQELAETLAETNRRLEQAQAEARRSERLAALGQLSAGLAHEIRNPLGVIKGS
                     AEMLNQKLGKADPLTAELAGYISSETNRLSALVSRFLDFARPLHADLALQDITAVLDR
                     ALSDVATIRKEAPIRIEKDYEPDLPAVPLDESLCQQAFVNIVQNAYDAMGEAGGLLRV
                     K"
BASE COUNT          549 a          928 c          860 g          495 t
ORIGIN      
        1 gggatcgctg atcctgcgcg tgcgccacgt cgacgacgac gacagctacg aagtggacac
       61 tccgaacatc gcttttaccc tgcgccagcc gggcgaatac cggctcgacg tcagcgccga
      121 tggcactcag accatcgcca ccgtctttca cggccgcggc cgggtcacgg gcggaggctt
      181 ctcctacacc gtcatcgcca atcaatcggc cacgtttacc ggaaatgacc agcatctcga
      241 ctacgatctg gcccagttgc ctcagcagga cgattttgac agctgggctt ttgatcgtga
      301 ccagcgcgaa gatcagtccg attccgccaa ctatgtatcc cgcgagatga ccggctacga
      361 agatctcgac gccaacggaa cctggagtta tgccgccggc tacggcgaag tttgggcgcc
      421 gaccgccgtc gcggtgggct gggcgcccta tcgcttcggt cactggatct gggtcagccc
      481 ctggggctgg acctgggtgg aagatgaacc ctggggcttc gctcccttcc actacggacg
      541 gtgggcattt ctcggcgcgc gctggtgctg ggtgccggga ccggtggtcg tgcgcccggt
      601 ctgggctcct gctctggtcg ccttcgtcgg cggtgggccg ggcttccact tctcggccgg
      661 cgtcggcgtc ggatggttcc cgctggggcc gggagaagtc ttcgtccccg gttaccacgt
      721 cagtcgtggc tatgtgaaca acgtcaatat caccaacacc acggtgaacg tcaccaaggt
      781 cacgaacgtc tacaacacgg tcatcgtgaa caaaacgacg accatcaaca acatcaccta
      841 cgtgaaccag cacgtaaaca acgccgtgac cgtggtgtcg catgacgcct tcgtgaactc
      901 gcggccggtc gcgcagaacg tgatgaaggt cgagccgcac gagatcgccg ctgcgcctgt
      961 cagccacgtc gttgccgccg aaccggtgcg caccagcgtc atgggcgcgg gacgtccggt
     1021 cgccgtcaag ccgccggccc cggtgatcaa ccgcccggtg gtcgcggtga gaacgccgcc
     1081 tccgctgccc catccgctcg atcagcgtca gaccgaggcc ggtgggcacc tgaatcagca
     1141 gttgctggtg cggccggcag gtcctgctcg cccagcgcag atgactccag cgacgcaaca
     1201 gcccgcggcg caaggaggat tcaagtcctt cgggcagccc agtcagccga gtagcggaaa
     1261 cgcgcaggtc aagcagatgc cgcagactca gccgcgcgtc tatgagcaac agggaactcc
     1321 gcagcccgaa ccccggcccg aaaatcggaa cgtggaggag cagaccaatc gcaacgtgca
     1381 gcctgaaaac cgcaaccttc agccgcagaa ccgcaacatg cagccggaaa accagagccg
     1441 ctcattccag cctccgccgc aggaaatgac gcatccgttg gtgcatccgg cgcctccggt
     1501 cgaagagcac aacccgcagg tggagcagca gaaggagcag aagttccagc agtggcagca
     1561 gcagcggccc ccggcctcgc agcccaagtc gcagccgcag ccaaggtcgg aaccgcccaa
     1621 gcaaagcgcc ccgccaaaga aaggacgctg agtctggctg aggttgtgcg ctgtgacggt
     1681 gcgctgtgat aaggttcgct ggagatgcgc ctggggaccc gataagctgc aacgatcacg
     1741 ccgggagcga caacctctcc cggcgtatcc tttttgcagc gttgtttttc gcgccagctc
     1801 gatgacaccg caatgacggc acgccaatga ccgacccgaa gcgctccacc atcaactgga
     1861 tggacggcct gtggctgatg ttcctggccg gcctggcctt gcttccgccg gtggaggagc
     1921 cccacaagca ggccatccta ctggcgttcg gagtcctcca gttggcggag agctggctgg
     1981 taacgcgcct gccccgccgc ggccctgcct atgtcgtgct gttgaaaatc gccctggcaa
     2041 ccctgctgat cgatcatacc aacgagatcg ccattaacag cagctactgg ccgatctact
     2101 ttctgcctgc agtcaccgct gccgagtatt tcggtccgct ggcgacactg ctctggacca
     2161 cactggcctc cgccgcttat tgttcgtacc tgtatcaggc ctctttcgaa ttcgaactca
     2221 ccgaggaagc ctacggtctt ctggcgattc gtgtcctctt cttctttctc gccgccatgg
     2281 tggtgaaccg ctacgtggtc gagagccgga agcagacaca gcgctatcag gagctggccg
     2341 agaccctggc agaaaccaac cgccgcctcg agcaggcgca ggccgaggcc cgacgctcgg
     2401 agcgtctggc cgccctgggc cagctctctg ccggccttgc gcacgagatt cgcaatcccc
     2461 tgggcgtgat caaaggctcg gcggaaatgc tgaaccagaa gctggggaag gccgatcccc
     2521 tgaccgcgga gttggccgga tacatttcca gcgagaccaa tcgtctgagc gcgcttgtga
     2581 gtcgcttcct cgatttcgcc cgccccctgc atgccgatct ggcgctacag gacataactg
     2641 cggttttaga tcgagccctg tccgatgttg cgactatcag aaaagaggcg cccattcgca
     2701 tcgagaaaga ctacgagccg gacttgccgg cggtgccgct ggatgaaagc ctctgccagc
     2761 aggctttcgt caacattgtg cagaatgcgt acgatgcgat gggagaagca ggcggtctgc
     2821 tgcgcgtaaa gg
//
LOCUS       DASPUL010000114         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_567387, whole genome shotgun sequence.
ACCESSION   DASPUL010000114 DASPUL010000000
VERSION     DASPUL010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567387"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1430)
                     /locus_tag="VEJ00_02910"
     CDS             complement(123..1430)
                     /locus_tag="VEJ00_02910"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HXX00137.1"
                     /translation="MNLKSGLVLALCLAFAGVSHAATELVIATVNNGHMIEMQKLTKF
                     FEQANPDIKVKWVTLEEGVLRQRVTTDIATKGGQFDIMTIGMYETPIWGKKGWLQELK
                     PDAAYDVDDLLPAMRQGLSVDGKLYAAPFYGESSMLMYRKDLADKAGVKLGDRPTWEQ
                     VKELAAKIHNPQAGVYGICLRGKPGWGDNMAFLTTLANTYGGQWFDMTWKPQLESKPW
                     KEAINFYVDLLNKYGPPGSSANSFNEILALFNEGKCGMWVDATIAASFISDPKQSKVA
                     DKVAFAQAPMAVTPKGANWLWAWALAIPSGSKNQEAAQKFISWATSKEYINLVGKEVG
                     WGSVPTGTRKSTYANPDFLKAAKFATAEKKAIDTANPNDSTLPKSPYVGVQFAAIPEF
                     QAIGIAVGQQMSAALAGKETVDQALKTSQTAADREMRKAGYYK"
     gene            1731..2540
                     /locus_tag="VEJ00_02915"
     CDS             1731..2540
                     /locus_tag="VEJ00_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HXX00138.1"
                     /translation="MTELVDRHNPSERQLKFREQYQARIHRLYSGPVHVGVMFAIGIA
                     AISWCVSRLEHATWEWLLVIPVFFFSNLFEWWIHRYVMHRLIDVWALRAIYDRHTRQH
                     HQYFTDNEMTVSSTREWRIIFFPWRALLTFMAFGTPFALALGWLLNPNAGYVLMITIV
                     GQYLIYETFHYCCHTRDNWFVRCMPFVNTIRRHHTAHHNYGIMMGYNLNLTFPIADWV
                     MGTSDLNRGLLGTIFNGYNEDHVKPELKPTIERFRRPDAPVTIDGPVQQAA"
BASE COUNT          490 a          866 c          833 g          575 t
ORIGIN      
        1 aggcgagtca gcggatcgac gcaccctccc cgttcgcgct gagcgcagca gcttacgctg
       61 ccagctcagt gtgaacagag agtgtttgtg cgatttcggt cggcgcgagc aagcgcgtca
      121 gcttatttgt agtaccccgc cttccgcatc tcgcggtctg ccgcagtctg ggaagtcttc
      181 agcgcctgat cgacggtctc tttgccggcc aacgccgcgc tcatctgctg ccccacggcg
      241 atgccgatag cctgaaactc cgggatcgcc gcgaactgga ctccgacgta aggtgacttc
      301 ggcagcgtgc tgtcgtttgg gttagcggta tcgatcgcct tcttttccgc cgtagcgaac
      361 ttggccgcct tcaggaaatc cggattcgcg tacgtgctct tgcgcgtacc cgtgggaacg
      421 ctgccccaac cgacctcctt gccgaccagg ttgatgtact ccttggaggt tgcccacgag
      481 atgaacttct gcgcggcctc ctgattcttg gaaccggagg gaatcgccag cgcccacgcc
      541 cacagccagt tcgcgccttt cggcgtcacg gccatcggcg cctgcgcgaa ggcgaccttg
      601 tccgccacct tgctctgctt cggatcgctg atgaacgaag ccgcgatggt ggcgtcaacc
      661 cacatgccgc acttgccttc gttgaacagc gccaggatct cgttgaagct gttggcagac
      721 gagcccggcg ggccgtactt gttcagcaga tccacgtaga aattgatggc ctccttccag
      781 ggtttggatt cgagctgcgg tttccaggtc atgtcgaacc actgtccgcc gtaggtgttc
      841 gcgagcgtgg tcaggaaggc catgttgtcg ccccagcccg gtttgccgcg caggcagatg
      901 ccgtagacgc ccgcttgcgg attgtggatc ttggcggcca gctccttgac ctgttcccag
      961 gtcgggcgat cgcccagctt cacgccggct ttgtcggcga gatccttgcg atacatgagc
     1021 atcgagctct cgccgtagaa cggcgccgca tacagcttgc cgtcgaccga caacccctgg
     1081 cgcatggcgg ggagcagatc atccacgtcg tatgcggcat cgggcttcaa ctcctgcagc
     1141 cagcctttct tgccccagat cggcgtctcg tacatgccga tggtcatgat gtcgaactgg
     1201 ccgcccttgg tcgcgatgtc ggtcgtcacg cgctggcgca gcaccccctc ttccagcgtg
     1261 acccacttca ccttgatgtc gggattggcc tgctcgaaga acttggtgag cttctgcatc
     1321 tcgatcatgt ggccgttgtt gacggtcgcg atgacgagct cggtggcagc gtgtgagaca
     1381 ccggcaaagg cgagacacaa tgccaggacg agacctgatt tcaggttcat gggaaccctc
     1441 ctccagatta atttttagtg gacccggtgc gaacgttggt cacatcggcg gtttttatga
     1501 taggcagcgt ggcgcgcgtg tctgatactc cgggaagggg ctttacatac tattttgcgg
     1561 gccggcagcc gccaaggcct gaaagaggtg aaaaatagta tgtgaggttc gcttcggccg
     1621 gatggcagtg ttttgcgtaa actagcgtca ctctcccacc accccagaat tcccctctcc
     1681 ccttgcggga gagggttagg gtgaggggta caagatatag ggaggcaggc atgaccgagc
     1741 tcgtcgatcg tcacaacccg tccgagcgcc agctcaagtt ccgcgagcag taccaggctc
     1801 ggatccatcg gctgtacagc ggaccggtgc acgtcggcgt catgttcgcg atcggcatcg
     1861 cagccatctc ctggtgcgtc tcacgcctcg agcatgcgac gtgggaatgg ctgctcgtga
     1921 ttcccgtgtt cttcttctcc aacctgttcg aatggtggat ccacaggtac gtcatgcatc
     1981 gcctgataga cgtatgggcg ctgcgcgcga tctacgaccg tcacacccgt caacaccacc
     2041 agtacttcac cgacaacgag atgacggtca gctcgacgcg ggagtggcgc atcatcttct
     2101 ttccgtggcg cgcgctactc accttcatgg cgttcggtac gccgttcgcg ctcgcgctcg
     2161 gctggctgct gaaccccaac gcgggctacg tcctgatgat caccatcgtg ggccagtacc
     2221 tgatctacga gaccttccac tattgctgcc acacgcgcga caactggttc gtgcgctgca
     2281 tgcctttcgt aaacaccatc cgccgccacc acaccgcgca ccacaactac gggatcatga
     2341 tggggtacaa cctgaacctc accttcccga tcgcggactg ggtgatgggc acgagcgacc
     2401 tgaaccgcgg cctgctgggc accatcttca acggctacaa cgaagatcac gtcaagcccg
     2461 agttgaagcc gaccatcgag cgcttccgcc ggccagacgc gccggtgacg atcgacgggc
     2521 cggtgcagca ggcggcgtga gataagcttt ttcccgtcct tttcaaggag gcggtaggcg
     2581 cgttggaggg cggaacaggg ctccctctgg gatgtgaccc cggaaacgcg ccgccaggcg
     2641 caggctggga tcacgagtgc ttggtgctgt cgcgacggaa tcgctgaaga acccgcggcc
     2701 tggcgcgaag cgacggggtg gttgctgagc tgcggcgcca tgctgacagc ttggtccaac
     2761 gccg
//
LOCUS       DASPUL010000115         6559 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_701225, whole genome shotgun sequence.
ACCESSION   DASPUL010000115 DASPUL010000000
VERSION     DASPUL010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701225"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3000
                     /locus_tag="VEJ00_02920"
     CDS             <1..3000
                     /locus_tag="VEJ00_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00139.1"
                     /translation="HLLVNEDARIDLTLKLGAVKAEVTVTGDAPMVSTTTRDISGLVE
                     ERQIKDLPLNGRSYDLLLTLNPGVVNFTSQKTGGTGISNSTTANNFAVSGNRPQQNLF
                     LLNGVEYTSAAENNMQPGGTSGMLLGVDAVREFNVQRDSYSAEYGKRPGGQVLIVTQS
                     GSNQWHGSIFGFLRNNALDAPNFFDQGDAPPFQRNQFGAALGGPLHHDKTFFFLNYEG
                     FRQNLHQTSAAFVPDLASRAKAVASVKPLLDLWPTPPAGAPDFNGIAQVFSSPLQTIR
                     EDFGTVRLDHIFSGKDTLAAVYTIDDGDDFTATPLNPYSADVVTLRQQVLSLEETHLF
                     SPSLLNTARFGYSRAGYFFTGEPTPGTPAEDVPGFLLGHQVGAVVVGGSAASNPQASI
                     GLAGSNNGSDLRIARNLFTYADQVSYTRGGHQLNFGLWFQQFQSNENIALSQFGQATF
                     SSLAQFLGGTVNTFLYDPAPTDMNWRSLFGAFYAEDVIRLLPQLTLSLGFRGEFSTGW
                     NEAHGRAANYTFDNNGVISSQPRIADSLFTENNATFLPQPRIGVAWSPFKSKSVLRAG
                     FGMYNDLQDALGYRADQNAPFNPVYSLSALPVKSLPIDPAAPVSPKAKLVPGGVQPDM
                     KTATLISWSLRFEQEVTPDLAFSVGYVGSHGYHELIGIDANEPKPVICPAAPCPAFFP
                     TWDPSKPATATNSPSIGFPIGSPLAGAPVPAGTFYIPAGSPRANASLANTWTWFSLGT
                     SSYNALQVDVRKRMSHGLSFRGVYTWSKVLDDGDSLNQTTAGNAPGLVSNPYDLRSDK
                     GPGTFDVRHLGVVNVIYELPFGRGKALAGDLQGLSNALLSGWSLASIITAQSSFPFTP
                     QLSYNPSNNGDTRNPVRPFLNPLFNGSVVTGNPNQWFDPAAFIAPPSLSGFYGNAARD
                     AYTGPSLTTWDLSVLKSTSLHERLSLQFRAEIFNLLDTANFNTPNLIVFTPPTAANPI
                     GSSGTAGVITATTTTSRQVQFGLKLVW"
     gene            3794..5281
                     /locus_tag="VEJ00_02925"
     CDS             3794..5281
                     /locus_tag="VEJ00_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00140.1"
                     /translation="MGSSVAESDTLLEASRIETSAFADLLNDRVDLVPGTKGSGKSAL
                     FRIFVDFLPDILLQQKKVVVAHGIQAPGDPVFHAYLDRFSELSENEFINFWCVYLVSL
                     AQEQFLKGTRYRKFLGAAKTQIEMFRQACAKARIPEIEARKSLKEILDWSLNVLSSWR
                     PKLKYKAPHGGGEIEVDLFGEKNEPVPQDIGREPSHSLPKYVNEIKEALEAVLKASGL
                     SLWLMVDRLDEIFPRRSEVEKTALRGLLHAMRYFSSASIRVKVFLRDDMLEQVVRAEG
                     GFTALTHVTARQADKLQWTEDQILSMVVKRFFANDRLAQRFGVDADQLDANVSSRRAA
                     FYKIFPPTVFKGSKQSPTIRWIFIRCADGRGVVTPRDVLDLLIRAIQKQQDIFAADPD
                     STSDHLISTAAIQYGFEQLSKRKRETYLEAEFPHLWKEMEKFVGGKTEYDEFALRSLL
                     GPNWKSTSDHLLAIGFFTRGKKGGEDLFSIPFLYRHGMNVTQGKA"
     gene            5278..6066
                     /locus_tag="VEJ00_02930"
     CDS             5278..6066
                     /locus_tag="VEJ00_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00141.1"
                     /translation="MISFSRRGLKCHFAALHRAYGAQHWWPAATPLEVAVGAILTQNT
                     AWVNVERALASLRRAGKLSLQGLRSTPRRELAQLVRSSGYFRQKARSLHNFARFVARQ
                     YGGSLARMMKPKARPVEPENTGHLRRQLLALKGIGPETADAILLYAGGHPLFVVDAYA
                     RRIFERHGLFPARTSYDDIRSAVEQALAAMKPAAARDKRMGHRPSRMSRARRSPLARK
                     LAEFHGLLVLAGKQHCFKNRPDCAHCPLKKFLPEDSRLRMQPGD"
     gene            complement(6043..6276)
                     /locus_tag="VEJ00_02935"
     CDS             complement(6043..6276)
                     /locus_tag="VEJ00_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00142.1"
                     /translation="MINQKRGHILPELVRFVSDPHLDAMTRSWIFDALRDISGERFGD
                     DPRQWREWYASISGQQQSAEDHGSLLSVAWLHP"
BASE COUNT         1292 a         2085 c         1869 g         1313 t
ORIGIN      
        1 catttgctgg tgaacgagga tgcccgcatc gatctcacgc tcaagctcgg cgccgtcaag
       61 gccgaggtca cggtcaccgg cgatgctccc atggtcagca ccaccacccg ggacatctcc
      121 gggctcgtcg aagagcgcca gatcaaagac ctgcctctga acggccgcag ctacgacctg
      181 ctgctcacgc tgaatccggg agtggtgaac ttcacgtcgc agaagaccgg cggtacgggc
      241 atctccaact ccaccaccgc caacaacttt gccgtctccg gcaaccggcc gcagcagaac
      301 ctgttcctgc taaacggcgt agagtacacc agcgccgccg aaaacaacat gcagcccggg
      361 ggcaccagcg gcatgctgct gggagtggat gccgtccgcg agttcaatgt gcagcgcgac
      421 tcctacagcg ccgagtacgg caagcggccc gggggccagg tgctcatcgt gacgcagtcg
      481 ggcagcaacc agtggcacgg cagcatcttc gggtttctcc gcaataacgc gctcgatgcc
      541 cccaacttct tcgaccaggg cgatgccccg cccttccagc gcaaccagtt cggcgcggcg
      601 ctcggcgggc cgctccatca cgacaagacc tttttcttcc tgaactacga gggattccgc
      661 cagaacctgc accagacatc ggcggcattc gttcccgacc tggcttcgcg cgccaaagcg
      721 gtggccagcg taaagccgct gctcgatctc tggcccacgc cgccggcggg cgcgccggat
      781 ttcaacggca tcgcgcaggt cttcagcagc ccgctgcaga ccatccgcga ggatttcggc
      841 accgtgcgcc tcgaccacat cttctccggc aaggatacgc tggccgccgt ctacaccatt
      901 gacgatggcg acgacttcac cgccacgccg ctcaaccctt acagcgcaga cgtggtcaca
      961 cttcgccagc aggtgctgag cctggaagag acgcatctct tctcgccttc gctgctcaat
     1021 accgcccgct tcggatactc ccgcgccgga tatttcttca ccggcgagcc cacgcccggc
     1081 acccccgccg aagatgtccc cggattcctg ctgggacatc aggtgggcgc ggtcgtggtc
     1141 ggcggcagcg ccgcctccaa tccccaggcc tccatcggcc tcgccggcag caacaacggc
     1201 agcgacctgc ggatcgcccg caatcttttc acctacgccg accaagtctc ctacacgcgc
     1261 ggaggccacc agctcaactt cggcctgtgg ttccagcaat tccagtcgaa tgagaacatc
     1321 gcgctcagcc agttcggcca ggcgaccttc tccagcctgg cgcagttcct gggcggcacc
     1381 gtcaacacat tcctctacga tcccgcgcct accgacatga actggcgctc gctcttcggc
     1441 gccttttacg ccgaagacgt catccgcctc ctgccgcagc tgacgctctc gctcggcttc
     1501 cgcggcgaat tttccacggg atggaacgag gcccacggcc gcgccgccaa ctacaccttc
     1561 gacaacaacg gcgtcatctc ctcccagccg cgcatcgctg actcgctgtt caccgagaac
     1621 aacgccacct tcctgcccca gccgcgcatc ggagtcgcct ggagcccatt caagtcgaag
     1681 agtgtcctgc gcgccggctt cggcatgtac aacgacctgc aggatgcgct cggctaccgc
     1741 gccgaccaga acgcgccctt caatcccgtc tacagcctct cagccctgcc cgtgaagtcg
     1801 ctgcccatcg atcccgccgc gcccgtctca cccaaggcaa agctggttcc cggaggcgtg
     1861 cagccggata tgaagacggc gacgctcatc tcctggtcgc tgcgcttcga gcaggaagtc
     1921 acgcccgacc tcgctttttc cgtgggctac gtaggctccc atggatacca cgagctcatc
     1981 ggcatcgatg ccaacgagcc caaaccggtc atctgcccgg cggcgccctg cccggccttc
     2041 ttccccacct gggacccgag caagccggcc acggccacca actcgccgag catcggcttc
     2101 cctatcggct cgccgctcgc cggagcgccc gtgccagccg gcacgttcta cattcctgcc
     2161 gggagcccgc gcgccaatgc cagcctggcc aacacctgga cctggttctc gctcggcacc
     2221 agctcctaca acgcgctgca ggtggatgtg cgcaagcgca tgagccacgg actttccttc
     2281 cgcggcgtct acacctggtc caaagtcctc gatgacggcg actcgctgaa ccagaccacg
     2341 gccggcaacg cgcccggcct ggtctcgaat ccttacgacc tgcgctctga caaaggcccg
     2401 ggcaccttcg acgtccgcca cctcggcgtg gtcaacgtca tctacgagct gcccttcggg
     2461 cggggcaagg cgctcgccgg cgatctgcag ggcctgagca acgcgctgct cagcggatgg
     2521 tcgctggcca gcatcatcac cgcgcaatcc agctttccct tcacccccca gctcagctac
     2581 aacccctcca acaacggcga cacgcgcaat ccggtgcggc ccttcctgaa cccgctcttc
     2641 aacggttcgg tggtcacggg gaacccgaac cagtggttcg atcctgcggc cttcatcgcc
     2701 ccgccctcgc tcagcgggtt ctacggaaac gccgcccgcg acgcctacac cggccccagc
     2761 ctcaccacgt gggatctctc agtgctcaag agcaccagtc tccacgagcg gctgagcctg
     2821 caattccgcg ccgagatctt caacctgctc gacactgcca acttcaacac gcccaacctc
     2881 atcgtgttca cgccgccgac ggcggcgaac ccgattggct cctccggcac cgccggcgtc
     2941 atcaccgcga cgactacgac ttcgcggcag gtgcagtttg ggctgaagtt ggtgtggtag
     3001 caggttgccc cactctttcg ccgtccattg gcacggattg caggactatc agccgtccct
     3061 tcgggactag ctcgttaaac catacctttc ccggcacttc gtgccgggct acattcacac
     3121 gtcccttcgg gactctcgca agagcaacga tgatctgtca tcccgagcga gagcgcggga
     3181 gcaatcgggc ttgggtttgg ttcgctagga atctctcctc aggtttggaa aatccccagc
     3241 ctatcgtcaa aaggccgacg ataaggctgg ggcaaccagc gattgtcgca ataaataatt
     3301 gggaatcgcg attccctttg ggagagggcc gtgtggcttg gactccggtc ttgatgggct
     3361 gtgggttttt taggccccgt ctacctggga cagtgtcctc ttccggagtg ggacgtcggg
     3421 cagaatgtct gcagccagtt ctgccaagtt tctcccaatg aagaggagaa aattggttct
     3481 tctcccctta agtctagaat atcaactctg caggggtaac tatgaaagac ggaggggagg
     3541 aaatttgtgt ttagaatcaa ggggttacgg atgagtttat agaatcgtta gcttgacagg
     3601 ctgattggcg taggttggga ggcgattttt tttttgaggt cttcatcgat gttaaccctt
     3661 tcgttatccc accttttgct acgcaaagga tggggcaccc ggcatcgcgt tgcgcgtgga
     3721 ggaggggccc ggtaacatcg acctagtgcc gcatagcaac aagagcctat ctaccctgct
     3781 tcagtcgatc gacttgggtt cctccgtcgc cgagtcggat acattactgg aggcctcgag
     3841 aatcgaaaca tctgcatttg ctgatctgtt aaacgaccgt gtggaccttg tgcccgggac
     3901 aaagggttcc ggcaagagtg ctttgtttag gattttcgtt gattttcttc ccgatatcct
     3961 tcttcaacaa aagaaggttg tggtggctca cggtatccaa gctcctggcg accctgtgtt
     4021 ccatgcctat ttggatcgct tttctgagct ttctgaaaac gagtttatca atttctggtg
     4081 cgtgtatctg gtctcgctgg cgcaggagca atttttaaaa ggcacacggt atcgaaaatt
     4141 tctcggtgcc gccaagacgc agattgagat gtttcgtcaa gcatgtgcga aggcgcggat
     4201 tcctgaaatc gaggcaagaa agtcgcttaa agaaatactt gattggtcgc taaacgtcct
     4261 ttcgtcttgg aggccaaaac tgaagtacaa ggcgccacat ggtggtggag aaatagaggt
     4321 cgatctgttc ggcgagaaga atgagccggt gcctcaggat atcgggaggg aacctagcca
     4381 ttccctgccg aagtacgtga acgaaatcaa ggaggcgctt gaggccgttc tgaaggcgtc
     4441 gggcttgtcc ttgtggctca tggtcgatcg ccttgatgag atttttccga gaaggtcgga
     4501 ggttgagaag acagcgcttc ggggccttct tcacgccatg cgctacttca gttcagcatc
     4561 catcagagtc aaagtgtttc tacgagatga catgcttgaa caggttgtga gagccgaggg
     4621 cggatttact gccctaaccc acgtgaccgc gcgacaagcc gacaagttgc agtggaccga
     4681 ggatcagatt ctttcgatgg tggttaaacg cttttttgcc aacgacagac tggctcaacg
     4741 gtttggagtt gatgcggacc aactagacgc taatgtctct agccgccgtg cggcctttta
     4801 taagatcttt cccccaactg ttttcaaagg ttcaaaacag agcccgacga ttcgctggat
     4861 ttttattcgc tgtgccgacg gacgtggtgt agtgactccc cgcgacgttc ttgatcttct
     4921 gatacgcgca attcagaaac agcaagacat tttcgcggct gacccggaca gcacttcgga
     4981 tcaccttatc agcacggctg caatccaata tggttttgaa cagctgtcca agcgtaagcg
     5041 ggagacctat ctagaggccg agttcccgca cctatggaag gaaatggaga aatttgtagg
     5101 gggcaagact gaatatgatg agtttgctct tcgaagcctc cttggtccca actggaagtc
     5161 cacaagcgat cacttgctgg ctataggctt ttttactagg gggaaaaagg gcggcgagga
     5221 cttgttttcc ataccgtttc tctatcgaca cggtatgaat gtcactcagg ggaaagcttg
     5281 atctcgttta gtcgacgggg gctgaaatgc cacttcgctg ccctgcatcg cgcctacggc
     5341 gcacagcact ggtggccggc ggcgacgccg ctggaggtcg ccgtaggcgc tatcctcacc
     5401 cagaacaccg cgtgggtgaa cgtggagcgc gcgctggcca gcctgaggcg cgccggcaag
     5461 ctctcgctgc aaggcttgcg cagcacgccc cggcgcgagc tggcgcagct ggtccgctct
     5521 tcggggtatt tccggcagaa ggcgcgcagc ctgcacaact tcgcccgctt tgttgcccgg
     5581 cagtacggcg gctcgctcgc gcgcatgatg aagccgaagg cgaggccggt cgagccggaa
     5641 aatacagggc atcttcgccg gcagctgctc gcgttgaaag gcatcggccc ggagacggct
     5701 gacgccatcc tgctctacgc cggcggccat ccgctgttcg tggtggatgc gtacgcgcgg
     5761 cgcatcttcg agcgccacgg attattcccc gcccgcacca gttacgacga tatccgcagc
     5821 gcggtggagc aggcgctggc agcgatgaag ccggctgcgg cgcgcgacaa acggatgggc
     5881 caccgcccct cacgcatgag ccgggcgcgg cgctctccgc tggcgcggaa gctggccgag
     5941 tttcacggat tgctggtgct ggcaggcaag cagcactgct tcaagaacag gccggattgc
     6001 gcccattgtc cgctgaagaa atttttgccg gaggattcga ggttacggat gcagccaggc
     6061 gactgagagc agcgagccgt ggtcttctgc gctctgctgc tggccgctga tggaggcata
     6121 ccactcgcgc cactggcgcg gatcgtcgcc aaagcgctcg ccgctgatgt cgcgcagcgc
     6181 gtcgaagatc caggagcggg tcatggcatc gaggtgcggg tcgctcacaa agcgcaccag
     6241 ctcgggcaga atgtgtccgc gcttttgatt tatcatttcg agggcctcat ccgagataaa
     6301 gacaaagaac gagactaacg ccggccgccc gcagcggcaa gttacttgag taatcctcgg
     6361 tattccccgg cgacttgcag attttgcagc ggttggcggc cggagaacat ctaagagtca
     6421 ggcgaaatca agctctgtta taatcgggct gtaagcgata gcaagcaagg agattgacta
     6481 atgtcttcaa caccaatgac cgcgaccgag acgccgcagg cgcggccgac aacgccggtt
     6541 tctctcactc ccgtcgcca
//
LOCUS       DASPUL010000116         6250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1934896, whole genome shotgun sequence.
ACCESSION   DASPUL010000116 DASPUL010000000
VERSION     DASPUL010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1934896"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            406..1914
                     /locus_tag="VEJ00_02940"
     CDS             406..1914
                     /locus_tag="VEJ00_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00143.1"
                     /translation="MAEDLRFELAILATVQGFYQKLMSGALGGDVPIPNGLDEDALRH
                     SERELPNTLTRMYRWLQLLDMAITPAMLRQALTPDTDSEVAEALLRYFVRRREPSDVN
                     RDKTDLIATFLYRHPRVPGQWEQSGYGLDGALPLSPFEIALIEILADTDAPSLPEEHV
                     QLLRRFDPLREESSHFRDFNALIDSGIVGRVRELKQWLDSSFYHPGVLATVAAYNAAF
                     GKKFDELFAKALTEIKTFGQALEEMGGTILTTVDGVEVTVEHVAAIEQNQMLAADYGS
                     TQEKFRRVSKLKKELDRRPPIRRSLLTPAVRASQGAGGAGAAPKAARAAAAAAKGRPA
                     AAPVFQPPTITPQQVSAEEGKILRVEESIRVFVRVADPKYRQVVPMRFFNLTLTAPEA
                     DAYGAAYLEEKSLRADVARNLIRLVSLSARIATELEELKRSQKMSSVWKLHADSVVVL
                     LDMAGTSTEEAGGVAKQAEQNGAGVAGKAIHQSVQNLRARTDIAAKTLANVS"
     gene            complement(2109..4937)
                     /locus_tag="VEJ00_02945"
     CDS             complement(2109..4937)
                     /locus_tag="VEJ00_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXX00144.1"
                     /translation="MWGSIATLNPPASCLIHAGEARLTAQQSRGTGVSMNKELFVSST
                     PHETKVGLVEDDLLAEIYLERENEYTLAGSIYKGKVTRVLPGMQSAFVDIGLERDAFL
                     YVSDFMDLEEQDEDLTDAVPANRGVQDMRPQEARPQQVTESAGGAAEASASTAAGEEL
                     EPAAAVVHSEPADAVSADVEGGTNESGNDGDHNRDRGGWRGRRRRRGRRGDGRERGGE
                     LRSARPADNAPSRPQSFRPAPSGPPAGYQPILLPGESISKYQRLAQPAPASEPENVPR
                     EQPSAEGVAPISATFPEDEPIFASAESVAEPLHEEHEDIREDVSESTPSASTSAEWGH
                     EFRRPEAAVAAAASWGGDGERATDHAMVEEVEEAVEERAATERPAVREEETPVFAAPG
                     TLEEETIDEEEVDTVRYESSSDEGYEEFEEETRAAGPAHFNGDARETVDEIHAASLVE
                     SGAAQAREEGAVETDEDSEELELEEAQAEAEAMLDAEARGNGTVDARAEVRAPAATAG
                     YTQRTERRRFDRSGGPRDRGRRGGRRFRRRESAPVPLISDLLKEGQEILVQIAKEPIG
                     KKGARITSHIALPGRFLVYMPTVNHTGVSRKIASEEERQRLKRIIQSERENGHGGFIV
                     RTAAQNASEDELRADIRFLKGLWNDIKTRAENSKPPALIYHDLNVVERVLRDQVTSDF
                     NVIWVDSEQEYERILRFFNRFQPALVKRVKLYTKETPLFEQFGLAEEMNKALKSKVWL
                     KSGGYIVINQTEALVAIDINTGKYVGKTQRLEDTIVKTNIDAVKEIVRQVRLRDLGGI
                     IIIDFIDMDERRNRQRVMQALEEELRHDRAPSKTLQFNDFGLVAITRKRVKQSLERTI
                     GAPCPYCQATGYVKSVNTVCNEIYVEMRKIAKHLEHEDVMLRVHPDVAKELKANNARL
                     LNEMEELTKRTIIVKSDPALHQEQFDIN"
     gene            complement(5293..>6250)
                     /gene="rodA"
                     /locus_tag="VEJ00_02950"
     CDS             complement(5293..>6250)
                     /gene="rodA"
                     /locus_tag="VEJ00_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HXX00145.1"
                     /translation="WILGGIVLMFVMSLVNYQALLDRVHWFYIGAIAALMAVLVFGTK
                     ALGAKRWIKMPGGNHFQPSEWVKLILILAVAKYFAEMRSRELTWGDFVKAGAIVGIPM
                     LLVLKQPDLGTALTYLPIAAMGMFLGGLQWRQGLAVVLVAVVGIGVVFYVPRVHILKS
                     YQKQRLTSYQDPDSDPQGSGYQVKQSMIAVGSGGLWGGKGSQTHGAFLPVPQTDFIFA
                     AFAEEHGFVGALGVLLLYFIVLMRLTQNAQTAPDRAGTFVVMGVVAVLSFHILVNVGM
                     VVGYMPVTGIPLPLMSYGGSSVLFMFLALGLVMNVRMRRFVN"
BASE COUNT         1136 a         2161 c         1716 g         1237 t
ORIGIN      
        1 catccgcaaa ggccgcgacc tcgagaagat tgtgctctcg cgcgccgtgc gctggcacat
       61 cgaagaccgc gtgctcctct acggcaacaa aaccgtggtt ttctgagccg agccgcaacc
      121 ccatcaccac ggaccaacgg agaacccctc tctagccacg gaccgtcatg tcggctgcat
      181 tggcgcatca gcgcaggctc acgacgtagt ccattgcttt ccccgtgcct ccgtgtctcc
      241 gtggtgaaaa atccttccac gcggaagtcc atgtacctcc gggaaatccc atctcacgcc
      301 gtgagaaatc ccgctcccac tagtaacttg caccgctcgt aaccttcgtt tttacttgaa
      361 cattagtaaa gacttagaag gaaactctcg ctccgggggc attttttggc cgaagatctg
      421 cgctttgaat tggccatcct ggcgaccgtc cagggctttt accagaagct catgtctggg
      481 gcactcggcg gcgacgtgcc gatcccgaac ggcctcgacg aagacgcttt gcgccactcc
      541 gaacgcgagc tacccaacac tctcacccgg atgtaccgct ggctgcagct gctcgacatg
      601 gccatcacgc ctgccatgct gcggcaggcg ctgacgcccg acaccgattc cgaggttgcc
      661 gaagccctgc tgcgttactt cgtgcgccgc cgcgagccca gcgacgtcaa ccgcgacaag
      721 accgacctga tcgccacttt cctttatcgc catccccgcg tccctggaca atgggagcag
      781 agcggttacg gcctcgacgg cgcgctgccg ctttcgccct ttgaaattgc cctgatcgaa
      841 atcctggccg acacagacgc gccctcgctg cccgaagagc acgtgcagct gctgcgccgt
      901 ttcgacccgc tgcgcgagga atcaagccat ttccgcgact tcaatgcgct catcgactcg
      961 ggtatcgtcg gccgcgtccg cgaactcaag cagtggctgg attcttcctt ctatcatccc
     1021 ggagtcctgg ccacggtcgc cgcctacaac gcggccttcg gcaagaagtt cgacgaactc
     1081 ttcgccaagg ccctcaccga aatcaagact ttcggacagg cgctcgaaga gatgggcggc
     1141 accatcctca ccacggtcga cggcgtggaa gtcaccgtcg aacatgtggc cgcgatcgag
     1201 cagaaccaga tgctggccgc cgattacggc agcacgcagg aaaaatttcg ccgcgtctcg
     1261 aagctcaaga aggaactcga ccggcgcccg cccattcggc gttcgctgtt aactccggcc
     1321 gtccgggcat cgcaaggtgc aggcggcgcc ggggccgcgc ccaaggcggc gcgggctgcc
     1381 gcggccgccg ccaaaggccg tcccgctgct gccccggtgt ttcagccgcc gaccatcacg
     1441 ccgcagcagg tttcggccga ggaaggaaag attctgcggg tcgaagaatc gattcgcgtt
     1501 ttcgtccgcg tcgccgaccc gaagtatcgc caggtcgttc ccatgcgctt cttcaacctg
     1561 accctgactg ccccggaagc cgatgcctac ggcgctgcgt acctcgaaga aaaaagcctg
     1621 cgcgctgacg tggcccgcaa tctgatccgc ctggtctcgc tgtcggcccg catcgccacg
     1681 gaacttgaag aattgaagcg cagccagaag atgtcatccg tttggaagct gcacgctgac
     1741 tcggtagtcg tcctgctcga tatggccggc acttccacag aagaggcagg cggggtcgcc
     1801 aagcaggccg agcagaacgg cgccggcgtg gccggcaaag ccatccatca gtcggtgcag
     1861 aacctccgcg cccggaccga catcgccgcc aaaaccctgg ccaacgtcag ctagactatt
     1921 tctcgaagca tcacgcggac aagcaacggg aagggcacga ctttagtcgt gccgcaaagc
     1981 gaccaactga tgcggcttta gccgctgagg gcgacgccct acccgaccct acctcggaga
     2041 tcgacaataa aaggcgggcg attttcgccc gcctcttcaa ctcgcaccga gtcacttgga
     2101 actcgcagct agttaatatc gaactgctcc tgatgcagtg ccggatcgct cttcacgatg
     2161 atggtccgct tggtcagttc ctccatctcg ttcagcagcc gtgcgttgtt ggctttcagt
     2221 tctttggcca cgtccgggtg cacccgcagc atcacgtcct cgtgctccag atgcttcgcg
     2281 atcttccgca tctccacgta gatttcgttg cacaccgtgt tcaccgactt cacgtacccg
     2341 gtcgcctggc aataggggca gggcgcgccg atggtgcgct ccagcgattg cttcacccgc
     2401 ttgcgggtga tcgccaccag cccgaaatcg ttgaactgca gcgtcttcga gggcgcccga
     2461 tcatgccgta actcctcttc cagcgcctgc atcacgcgct gccggttgcg ccgctcgtcc
     2521 atgtcgatga agtcgataat gatgatcccg cccagatcgc gcagccggac ctgccgcacg
     2581 atctctttca ccgcatcgat gttggtcttg acgatggtgt cttccagccg ctgtgtcttg
     2641 cccacatatt tgccggtatt gatgtcgatc gccaccagcg cctcggtctg gttaatgacg
     2701 atgtatccgc cgctcttcag ccagactttc gacttgagcg ccttgttcat ctcctcggcc
     2761 aggccgaact gttcgaacag cggcgtctct ttcgtgtaaa gcttgacgcg tttcaccagc
     2821 gcaggctgga agcggttgaa gaagcgcaga atcctctcat actcctgttc agagtcaacc
     2881 caaataacgt taaaatccga cgtgacctgg tcgcgcagca cccgctccac cacattgaga
     2941 tcgtgataaa tcagcgcggg cggcttcgag ttttccgcac gcgtcttgat gtcgttccac
     3001 aatcccttga ggaaccggat gtcggcgcgc aattcgtctt cgctggcgtt ctgcgccgcg
     3061 gtgcgaacga tgaatccgcc gtgcccgttc tcgcgctcgc tctggatgat tcgtttcagt
     3121 cgctgccgct cttcctcaga agcgatcttg cgcgacacgc cggtgtggtt cacggtcggc
     3181 atgtagacca ggaagcggcc gggcaaggcg atgtggctgg tgatgcgagc gcctttcttg
     3241 cctatcggct ccttggcgat ctgcaccagg atttcctgcc cttccttgag caggtcgctg
     3301 attagcggca ccggagctga ttcgcgcctc cggaagcgcc gcccgccgcg tcgtccgcgg
     3361 tcgcgcgggc cgcctgagcg gtcaaaacgc cggcgctcgg tccgttgggt gtaaccggcg
     3421 gtcgccgccg gagcgcgcac ttccgcccgc gcatcaaccg tgccgttgcc gcgagcttcc
     3481 gcatcgagca tagcctccgc ctcggcctgc gcttcttcga gttcaagctc ctcgctgtcc
     3541 tcgtcggtct cgaccgcgcc ctcttcccga gcctgcgcag cgcccgattc caccagcgat
     3601 gcggcatgaa tctcatcgac cgtctcccgc gcatcgccgt taaagtgcgc cgggccagcc
     3661 gcacgcgttt cttcctcgaa ctcttcgtag ccctcgtccg aagaggattc gtaccgcaca
     3721 gtgtccactt cttcttcgtc gatggtttct tcttccaggg ttcccggtgc ggcgaacacc
     3781 ggagtctctt cctcgcgtac tgcgggccgc tcagtcgctg ctctctcctc aaccgcctcc
     3841 tcgacctcct cgaccatcgc atggtcagtt gcgcgctcgc cgtccccgcc ccagctggcc
     3901 gccgcagcca cagctgcttc cggacggcgg aactcatggc cccactccgc cgaggtcgaa
     3961 gccgaaggcg tcgattctga aacgtcctcc cggatgtctt cgtgctcctc atgcaggggc
     4021 tctgccaccg attcggccga ggcaaagata ggctcatctt ccgggaaggt cgccgaaatt
     4081 ggcgctaccc cctccgcgga gggctgttcc ctcggcacgt tctccggctc actggccggc
     4141 gctggctgcg caaggcgctg atacttcgag atcgattctc ccggcaacag gatgggctga
     4201 tatccggcag gcggcccgga tggcgccggc ctgaagctct gcggacgaga cggtgcattg
     4261 tccgcgggtc gcgcggagcg cagttctccg ccccgctccc ggccatcccc ccgccgcccg
     4321 cgacggcggc gtcttccgcg ccagcctcca cggtcgcggt tgtgatcgcc gtcgttgccg
     4381 gactcattcg ttccaccctc cacgtcggcc gataccgcat ccgccggctc gctgtgcaca
     4441 actgctgccg ccggctccaa ttcctcgccc gcggcggtcg atgcggacgc ctctgcggcc
     4501 ccgccagcgc tctccgtgac ctgctgcggc cgcgcttcct gcgggcgcat atcctgcacg
     4561 ccgcgattcg ccggcaccgc atcggtcaaa tcctcgtcct gctcttccag atccatgaag
     4621 tcggagacat acaggaaagc gtcgcgttcg aggccaatat ccacgaatgc cgactgcatg
     4681 ccgggcagca cccgcgtcac ctttcctttg tagatggatc ccgctaaggt gtattcgttt
     4741 tcgcgctcga ggtagatttc cgcgagcaga tcgtcttcca ccagccccac cttggtttcg
     4801 tgaggcgtgg aggacacaaa cagttccttg ttcattgaca ctccggtccc gcgcgactgt
     4861 tgtgcagtca ggcgggcttc accggcgtga atcaggcaag aagcgggggg attgagcgtg
     4921 gcgatgctgc cccacatggg ggacgccctg ggccaaggag ttctcctcct ggcctagttc
     4981 gttgcacaag cttgggagtc ttttgcactt atcgcattca cttccgccgg gttttgtgac
     5041 cgcactctca gaccgtcatt tcggtcgggg ggccgtttcc ctgcccggct gttcgaagct
     5101 tcaatcccgc ccggcctcaa ctgctcattg gccggtgaat gccctcttta cgcgagggcg
     5161 atcgatattt ttgggctgcg ccctcggtct ttgccccaat cggaccggac cgaaccaggc
     5221 acagcaactg ctttcctgcc gcctcccccg ttcattgctc ctgcgactag ctcgaactgc
     5281 tggctcaccc agctagttaa cgaaacggcg catgcggacg ttcattacca gacccagcgc
     5341 caggaacatg aacaatacgg aagacccacc ataactcatt agcggcaggg gtattccggt
     5401 gaccggcata tagccgacca ccatgccgac attgaccaga atgtggaagc taagcaccgc
     5461 taccacaccc atcaccacga acgtgccagc gcggtcgggc gctgtttgcg cgttctgggt
     5521 caaacgcatc agcactatga agtatagcag cagaacccca agcgcgccta caaacccatg
     5581 ctcctcggca aacgccgcga agatgaagtc cgtctgcggc accggcagga aagccccgtg
     5641 tgtttgcgag cctttcccgc cccacagtcc cccggaacct accgctatca tggattgctt
     5701 aacttgatac cccgacccct gcggatcaga atcgggatcc tgataactcg tcagccgttg
     5761 cttctgatac gacttcagaa tgtgcaccct gggaacgtaa aagaccaccc caattcccac
     5821 caccgcaacc aaaacgacgg ccaaaccctg ccgccactgc agcccgccca ggaacatccc
     5881 catggctgcg atcggaagat aggtgagagc cgtccccaga tccggctgct tcagcaccag
     5941 cagcatcgga atgccgacga tcgcacccgc cttgacgaag tcgccccagg taagctcccg
     6001 cgaccgcatc tcagcaaaat atttggcgac ggccagaatc agaatcagct taacccactc
     6061 cgagggctgg aagtgattgc cccccggcat cttgatccag cgcttggccc ccagcgcctt
     6121 ggtgccgaat accagcaccg ccatcagcgc agcaatcgcc ccgatgtaga accagtgcac
     6181 ccggtccagc agcgcctggt agttcaccag gctcatcacg aacatgagga caatcccgcc
     6241 cagaatccag
//
LOCUS       DASPUL010000117         4090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_291299, whole genome shotgun sequence.
ACCESSION   DASPUL010000117 DASPUL010000000
VERSION     DASPUL010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291299"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VEJ00_02955"
     CDS             complement(<1..1239)
                     /locus_tag="VEJ00_02955"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXX00146.1"
                     /translation="MKRWVLLSGIVVLGIAAVVVSERQKVDVSASPAAVLYLVADTEQ
                     ELTRMPVHFTRMSDDEEIRIGDELARVYAGSRSDENKAPSTLAVERYLNQVGGPLALR
                     AHRRLPYRFHYIPGQYMVNAFALPGGHVYVGGGLLALMDSEDELANVIGHEIEHVDHY
                     HCAERAQQEEALRKIPLGGLVAIPIAVFEAGYSKDQELEADREGTRLAVEAGYSPNGA
                     IRMFEAFERLYRQYQSDQARAKTPQEEVSRLTLETLEGYFRSHPLPSERIAQIQKLIA
                     SEGWPVRSERELAVAYIFWTVRAQTALDSKKYQEAERLAQQSLKMHPDQPEAWDVLAQ
                     AQFAQAKFAEAATSYRKLLESNTRNDPKKLTSVAAYAMALAAADRNTGAAEFRSWLES
                     VQGARPRNAEVEQAGLSLLAG"
     gene            complement(1475..>4090)
                     /locus_tag="VEJ00_02960"
     CDS             complement(1475..>4090)
                     /locus_tag="VEJ00_02960"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HXX00147.1"
                     /translation="DRFRSTLDIEEMLGTNWLNKIGIALVVLGVAFFLQLALKSMGPA
                     GKVMVGFVTAAVMLGAGVFFERGDRYRILARAGIGGGWALLFFTTYAMYHVPAAQVLD
                     SQPTDLALMLVVAAAMVGHTLRYRSQVVTGLAFLLAFLTVTISHSNVYSLSAGGVLAA
                     GLVVIVGRMQWFELEVFGILASYLNHYLWLRPIIEPMHGKRHPFPEFAASAGILALYW
                     LVFRFSYVFRRPERDEQERISTAAALLNTALLLALFKYQSAHPEWAFWALLAIGGLEL
                     AFGQLPAARRRRTAVIVLCTLGVVLLIAAFPFRYSGTRLSVLWLAEAEALLLIGVWTK
                     EIVFRRLGVIASVLVAGQMLAYDAAAVVGRRMDGADLRPDFPLAILFVVAGTVFYLNT
                     HWVLRRWHELFTTQIDRTTMQRLSYAGAVMLLVAAWIAFPESWTAVAWCALALALTLA
                     GKRLAARDLAVQANLLAVTALVRVLAINLEATDKLHGLTLRLITITLVAALLYVTSRW
                     SGKPDRQEEGQWSFEGIVADGYVWAGSFLLALLAWYELRPVGVADAWVVGGLVLLELG
                     LTRKSVSLRLQAHVALAATFLRIFVVNLNAAGAPGEVSPRFYTVVPIALAFFYAYWRL
                     EAERESLSRLEQKLHSGSVCCWLGTISVGSLMRFELEADWVAAAWAALAFALVAIAWR
                     SGRKVFLHQAVLVAIGVLFRTVLHNFYERSYFPAPFWESRWVTVGATVALLLASLPIA
                     FQVRKKGEPSEKVGIPRLLQALARRPEQLFFFLAIGSLTVLLALEMRHGMVTLSWGLE
                     AVAVFLTALWLRERSFRLTGLGLLLLCVGKIVVADVWRLNPRDRYVTFIVLGCAVLLV
                     SFLYTRNREALRRYL"
BASE COUNT          866 a         1282 c         1268 g          674 t
ORIGIN      
        1 gccggccagt aaactcaggc cggcctgctc gacctcggca tttcgcggtc tagctccttg
       61 caccgattcc agccagctcc tgaattcggc cgcgccagtg ttccggtcgg cggcggccag
      121 agccatcgca taggccgcta cgctagttag cttcttgggg tcgttcctgg tgttgctctc
      181 cagcagcttg cgatatgaag tggcggcctc ggcgaatttg gcttgcgcga actgtgcctg
      241 cgccagcaca tcccaagcct cgggctggtc cggatgcatt ttcagcgact gctgcgccag
      301 gcgttcggct tcctgatact tcttgctgtc gagggccgtc tgggcgcgga cggtccagaa
      361 aatatatgcc accgccagtt cgcgctcgga gcgcactggc cagccctcgc tcgcgatcag
      421 cttctgaatc tgggcgatgc gctcggaggg cagcggatgc gagcggaaat atccttcgag
      481 cgtctcgagc gtgagtcgcg agacttcttc ctgcggagtt ttcgcgcggg cctgatcgct
      541 ctgatactgc cggtaaaggc gctcgaaggc ctcgaacatg cggatcgcgc cgttgggtga
      601 ataacctgcc tcgacggcga ggcgggtgcc ttcgcgatcg gcttccagtt cctgatcctt
      661 gctgtagccg gcttcaaaca cggcgatcgg gatggcgacc aggccgccga gcggaatctt
      721 gcgcagtgcc tcttcctgct gcgcgcgttc ggcgcagtga tagtggtcaa cgtgctcgat
      781 ctcgtggccg atcacattcg cgagttcgtc ttcgctgtcc atgagcgcga gcagtccgcc
      841 gccgacatag acgtggcctc cgggcagggc gaaggcgttg accatgtact ggccgggaat
      901 gtaatggaag cgatagggaa gacggcggtg cgcgcgcaag gccagaggcc cgcccacctg
      961 gttgagatag cgctcgacgg caagcgtgct gggggccttg ttctcgtcgg agcgacttcc
     1021 ggcgtaaacg cgagccagct cgtcgccgat gcggatctcc tcgtcatccg acatgcgggt
     1081 gaaatgcacg ggcatgcggg tgagttcctg ctcggtgtcg gcgacgagat agagcacggc
     1141 cgctgggctg gccgatacat cgaccttctg gcgctcgctc acgaccacgg cggcgatgcc
     1201 cagcacgacg attcccgaca acaagaccca gcgcttcatg attctggtac ctgaacctgg
     1261 ctgcgcgtcg cgcaatcgag cgactggcac agcgtttctc gcgatcgagc ccgttgcgtg
     1321 cgtagcggag aaagagcttc taaccgcaaa gatcgcgaag aaatgccgcg aaggtcgcag
     1381 agaaattcga tcttgccgtc tttcttggtc acctgccagc tacctgctct actttcctag
     1441 actcaaacta gttcagacat aagaaaccag cactctacaa atacctccgc agcgcctcgc
     1501 ggttgcgcgt atagaggaaa gaaaccagca acaccgcaca gcccagcaca atgaacgtca
     1561 catagcggtc gcgggggttc agccgccaca catcggcaac cacgatcttg ccgacgcaga
     1621 gcaggagcag tccaagcccg gttaaccgga agctgcgttc tcgcagccac aacgcggtga
     1681 gaaacacggc cacagcttcc agcccccatg acagcgtgac catgccgtgg cgcatttcaa
     1741 gggcgagcag cacggtcagc gatccgatcg cgaggaagaa gaaaagctgt tcgggacgtc
     1801 gtgccaaagc ctgcaacagg cgcgggattc cgaccttctc gctgggctcg ccctttttgc
     1861 gtacctgaaa cgcaatgggt agcgaggcca gcagcagggc gacggtcgcg cccaccgtga
     1921 cccagcggct ctcccaaaaa ggggcgggaa agtagctgcg ctcgtagaaa ttgtgcagca
     1981 ccgtgcggaa caaaacgccg atagcaacca gcactgcctg atgcaggaag acttttcgcc
     2041 cgctgcgcca ggcgattgcg acgagcgcga acgcgagcgc agcccaggct gcggcaaccc
     2101 agtccgcctc gagctcgaat cgcatgagcg atccgaccga gatcgtcccc agccagcaac
     2161 atacactccc tgagtgcaac ttctgctcaa ggcgggacag actttcccgc tccgcttcaa
     2221 gccgccagta ggcatagaaa aacgcgaggg cgattgggac gacggtgtag aagcgcggac
     2281 tgacttctcc cggtgcaccc gcggcgttca ggttcacaac gaaaatgcgc aggaaagtcg
     2341 cggccagcgc gacgtgagcc tggagccgca agctgacact ctttcgcgtg agccccaatt
     2401 ccagcagcac cagcccaccg acgacccagg cgtcggccac accgaccggg cgcagttcgt
     2461 accaggccag caacgccaac aggaaggacc cggcccaaac gtagccgtca gcgactatcc
     2521 cctcgaacga ccattggcct tcctcttgtc ggtccggttt cccgctccag cgcgaggtca
     2581 catacagtag cgctgcgacg agcgtgatcg tgatcagccg cagcgtcagg ccgtgcagct
     2641 tgtcggttgc ctcgagattg atggctagga cgcgaactaa cgccgttacg gccaggaggt
     2701 ttgcctgaac tgcgagatcc cgtgctgcca agcgctttcc ggccaatgtc agggcaagtg
     2761 ccagagcgca ccaggccacg gcggtccagg attcggggaa ggcgatccac gccgccacca
     2821 gtagcatgac agcgcccgcg taagaaagac gctgcatggt ggtgcggtcg atttgcgtag
     2881 tgaacaattc atgccagcgc cgcagaaccc aatgcgtgtt caggtagaaa accgttccgg
     2941 cgacgacaaa caaaatggcg agggggaaat cgggacgcag gtcggcgccg tccatgcggc
     3001 gtccgacaac cgcggcggcg tcgtaggcga gcatctggcc ggcaaccagc accgacgcga
     3061 tcactcccag gcggcggaag acgatttcct tcgtccagac cccgatcagc aggagtgcct
     3121 cagcctcggc cagccacagg accgacaggc gtgttccgga atagcgaaac gggaaggccg
     3181 caatgagcag gaccacgccg agcgtgcaca gaacgatcac agcagtgcgc cggcgacgag
     3241 cagcaggcag ttgcccgaac gccaattcca gtccgccgat cgccagcagc gcccagaagg
     3301 cccactcggg atgcgcagac tggtatttga acagggccag gagcagcgcg gtgttcagca
     3361 atgccgccgc ggtggagatg cgctcctgct cgtcacgctc cggccgacgg aacacgtaag
     3421 agaagcggaa gacgagccag tacaaggcca gaattccggc gctggccgcg aattcgggga
     3481 aagggtgacg cttgccgtgc atgggttcga tgatcggacg cagccacagg tagtgattca
     3541 aatagctggc caggattccg aagacttcga gttcgaacca ctgcatgcgg ccgacgatga
     3601 ccaccaggcc cgcggcgaga acgcctcccg cggacaagct gtagacattg gaatggctga
     3661 tcgtgacagt cagaaacgcc agcaggaaag ccagcccggt tacgacctgc gagcgataac
     3721 gcagcgtgtg cccgaccatg gccgcggcca caacaagcat cagcgccagg tcggttggct
     3781 gggaatcgag aacctgcgca gcgggcacgt gatacatcgc gtacgtggtg aagaacagga
     3841 gcgcccatcc gccgccaatt ccggcgcgcg cgaggatgcg atagcgatct ccgcgctcaa
     3901 aaaagacgcc cgctcccagc atgacggccg cagtgacgaa ccccaccatc accttgccgg
     3961 cgggacccat gctcttgagt gcgagttgca ggaaaaaggc gaccccgagg acaaccaggg
     4021 cgattccgat cttgttgagc caattggtgc ccaacatctc ttcgatatcg agggtggacc
     4081 tgaaacggtc
//
LOCUS       DASPUL010000118         6631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_428325, whole genome shotgun sequence.
ACCESSION   DASPUL010000118 DASPUL010000000
VERSION     DASPUL010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428325"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VEJ00_02965"
     CDS             complement(<1..326)
                     /locus_tag="VEJ00_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00148.1"
                     /translation="MSIHPTAIVDAQAKVPASCTVGPYCVIGADVELGERCHLLSHVA
                     VAGPTKIGSDNAFFPFCAIGMDPQDVTYKGERTRLEIGDHNEIRECVTINRGTLKGGG
                     LTKIGS"
     gene            complement(373..918)
                     /gene="fabZ"
                     /locus_tag="VEJ00_02970"
     CDS             complement(373..918)
                     /gene="fabZ"
                     /locus_tag="VEJ00_02970"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF000582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HXX00149.1"
                     /translation="MTESTSAVTETPAAGAKIVLDIHEILKILPHRYPFLLIDRVLEL
                     KRKERIVALKNVTINEPFFAGHFPGLPIMPGVLIVEAIAQAGGALLLTEVDDRHDKVM
                     VFTGIDRARFRKPVGPGDQLRLEVEVKGWRAVPGLTAARMQGYAYVGSVRVAEAGVSC
                     QLIESWRGRRSNHESGNGEGE"
     gene            1003..2184
                     /locus_tag="VEJ00_02975"
     CDS             1003..2184
                     /locus_tag="VEJ00_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXX00150.1"
                     /translation="MPRMKTQRAATQADKAWLERLRYCESREQALVKTIRQFVEIESP
                     SDDKPAGDRMSAFLAGAFEALGGRARIHAAADAGNNLQVDFAGREKTKPVLLLGHFDT
                     VYPLGTLATMPCREADGRLYGPGVLDMKSGIALMLYAIEALQAWHSGLPRPVTVFLVS
                     DEEIGSRSSRKITEALARESAAVLVLEPAGPRGAVKTARKGVGEYKLTVRGVASHAGL
                     DPGKGHSAVLELARQMLALAKLNNVKSGVSVNPGVIRGGTRTNVVAAEAVAEIDVRIV
                     HARQASEVDRKLRSLKPVDKRCKLTIEGGINRLPMERNGGVASLYQKAQGVAKHVGWH
                     LEEAAVGGGSDGNFTAGIGIPTLDGMGGMGDGAHAVHEHIVISELPRRMLLLAGMIET
                     I"
     gene            2201..2524
                     /locus_tag="VEJ00_02980"
     CDS             2201..2524
                     /locus_tag="VEJ00_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HXX00151.1"
                     /translation="MLKFMVVLYRRPELTPAEFRRHLEQVHGPLAKALPGLRRYIQNY
                     PCTDAKRKSPGWDAIVELYFDDWAAMEAAWASPQGAASDADLPAFTDLTRTTWSAVEE
                     VTVAP"
     gene            complement(2508..2882)
                     /locus_tag="VEJ00_02985"
     CDS             complement(2508..2882)
                     /locus_tag="VEJ00_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00152.1"
                     /translation="MTSIPKRSATSTSGSNKEQLGNVKTILSEPDDPLLPARSVDAVL
                     LLKTYHEVAQPVVLLRKLRGALRPGAKVGVIDRNGNGENHGVGRDVVIREAKEAGYDL
                     LAQYDFVKKDQMDYFLVFTAQP"
     gene            3426..3608
                     /gene="tatA"
                     /locus_tag="VEJ00_02990"
     CDS             3426..3608
                     /gene="tatA"
                     /locus_tag="VEJ00_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HXX00153.1"
                     /translation="MGEFSIWHWLLVIAIALLLFGPSKLGSLGKGLGEGIRNFRTAMK
                     EGAEEKKDEKPEEKKS"
     gene            complement(3659..4255)
                     /locus_tag="VEJ00_02995"
     CDS             complement(3659..4255)
                     /locus_tag="VEJ00_02995"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HXX00154.1"
                     /translation="MLVLASASPRRQELLRSAGIPFSVEPANVDETPFAGESPRDCAE
                     RLAREKALAVFAARPHDRVLGADTVVAVDETILGKPVDGEDAARMLRLLSGRVHRVIT
                     GVCLVEAGPGRQGSGGSHITTASETTLVTMNELSEEDIRGYVATGEPMDKAGAYAIQG
                     MAARFIPRIEGDYSNVVGLPVALVLRILRERSGDAEKA"
     gene            complement(4280..4651)
                     /locus_tag="VEJ00_03000"
     CDS             complement(4280..4651)
                     /locus_tag="VEJ00_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXX00155.1"
                     /translation="MPLPPFAAIDHVQLAMPPGGEDAARRFYCDLLGMNEIAKPPELA
                     KRGGCWFESGTVQIHLGVEPDFRPARKAHPALLCSDYDALTARLRAAGVETREDDSIP
                     GVRRCRIPDPFGNRIELIRSS"
     gene            complement(4729..6183)
                     /locus_tag="VEJ00_03005"
     CDS             complement(4729..6183)
                     /locus_tag="VEJ00_03005"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXX00156.1"
                     /translation="MRRIMISVLLGSSLAAAQTLSPEVRQFVKIDAPVVALTHVRVID
                     GTGAPAREDQTVILNKGKIESVSDASSASIPKDAQVLDLHGYSVIPGLVGMHEHMFYP
                     AGDAVFHEMAISFPRLYLAGGVTTIRTAGSIEPYMDLALKKQIDSGQSIGPRIHVTGP
                     YLEGPGSWAPQMTALNTPEEAVATVTFWADRGVDNFKAYNFITRAVLKAAIDAAHKRG
                     LKVTGHLCSIGFREAAALGIDDLEHGITVDTEFYSGKKPDQCPIDSMATMAGVAVETG
                     RMRVSDAPIQEMIRDLVAHHVAVTSTLPVFELFVPDKTPLQQRVLDAMSEEARNQFLV
                     NRVQADLPIKDPKTGEVRQAPWRRMFEQEEEFEHAFAQAGGLLIAGEDPTGMGGDLAG
                     FGDQREVELLVEAGFTPLEAIHIATFNGAQYLGELDRIGTIAAGKQADLVVIKGDPSK
                     KIDDIENVETVFKEGIGYDSAKLIESVRGLVGIR"
     gene            complement(6217..>6631)
                     /locus_tag="VEJ00_03010"
     CDS             complement(6217..>6631)
                     /locus_tag="VEJ00_03010"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXX00157.1"
                     /translation="IGPNVALTLIVGVALGAIIWKHPPAAWSPLQIVGACLLAAGFTL
                     WTVARFQLGRSLTVTAQAKQLVTGGLYSRIRNPIYTFGSCVIAGLILVLGRPIWLLVF
                     VLIIPLQLWRARKESQVLEAKFGDEYRKYRAATWF"
BASE COUNT         1311 a         2001 c         2001 g         1318 t
ORIGIN      
        1 tggctgccaa tcttcgtcag tcctccgcct ttcagcgtac cgcgattgat ggtcacgcac
       61 tcgcgaatct cgttgtgatc accgatttcg aggcgcgttc gttcgccctt gtaagtcaca
      121 tcctggggat ccatgccgat ggcgcaaaaa gggaagaacg cgttgtctga gccgattttc
      181 gtcggtccgg cgacggcgac gtgtgacaac aggtggcacc gttccccgag ctcgacgtcg
      241 gctccaatca cgcaatacgg tccgactgtg cacgacgcgg gcaccttcgc ctgcgcatcg
      301 acaatggccg ttggatgaat actcatggag gatcgattca ccgaatcgct ggcgttagag
      361 aaggtttgcc gattactcgc cttcgccgtt tccgctttcg tggtttgatc gccgccctcg
      421 ccagctctcg atgagttggc acgacacgcc ggcttcggcc acgcgtacgc tgccgacgta
      481 ggcataaccc tgcatccgtg ccgctgtcag gccaggcaca gcgcgccagc ccttcacttc
      541 cacttccagc cgcagctggt cgcccggacc caccggtttg cggaatcttg cccgatcgat
      601 gccggtaaac accatgactt tgtcgtgccg gtcgtcaacc tcggtcagaa gcagtgctcc
      661 gccggcctgg gcgatggcct cgacaatcaa gacgcccggc atgatcggca gaccgggaaa
      721 atgtccggcg aagaaaggtt cgttgatggt gacgttcttg agggcgacga tgcgctcctt
      781 gcgtttcagt tcgagaacgc ggtcaatcaa caggaaagga tacctgtgcg gcagaatctt
      841 caggatttcg tgaatatcca gcacaatctt cgccccggca gccggcgttt ccgtcacggc
      901 cgaagtgctc tcagtcatcg attcgtcccg aacggcttga agattatact gtacgcctta
      961 cgcccgagcc ggaccttccg gtctggaaca gcggaagaca ctatgcctcg catgaaaaca
     1021 caacgcgccg caactcaagc cgacaaggcg tggcttgaac gcctgcgata ctgcgaatcc
     1081 cgcgagcagg cgctggtcaa aaccattcgc cagttcgtcg aaatcgaatc gccaagcgac
     1141 gacaagccgg ctggggaccg gatgtcggcg tttctggccg gcgccttcga ggccctggga
     1201 gggcgtgcac gcattcacgc cgcagcagac gccggcaaca acctgcaggt ggatttcgcc
     1261 gggcgcgaga agacaaagcc tgttttgctg ctgggccact ttgacaccgt gtacccgttg
     1321 gggacgctgg cgaccatgcc ctgccgcgaa gccgacggac gcctctatgg gcccggtgtg
     1381 ctcgacatga agtcgggcat cgcgctgatg ctgtatgcga tcgaggcgct gcaagcgtgg
     1441 cactcgggcc tgccgcgacc ggttacggta ttcctggtct cggacgagga aattggcagc
     1501 cgttcctcgc gcaagattac ggaggcactg gcgagagaat cggccgcggt gcttgtattg
     1561 gaacccgcag gaccgcgcgg ggcagtgaag accgctcgca agggcgtcgg agagtacaaa
     1621 ctaacagtgc ggggagtagc ctcgcacgct ggcctcgatc cgggcaaggg acacagcgcg
     1681 gtcctcgaac tggcgcggca gatgcttgct ctagcgaaat tgaacaacgt gaaaagtggt
     1741 gtgtcggtga atcccggagt gattcgtggt ggaactcgca ccaacgtggt cgcggccgag
     1801 gccgttgcgg aaatcgacgt acgcatcgtt catgccaggc aggcgagtga ggtcgatcgc
     1861 aaactccgtt ccctcaagcc cgtggacaag cgttgcaagc tcaccatcga aggtggaatc
     1921 aaccgtttgc ccatggagcg gaatgggggc gtggcttcgc tctaccagaa agctcagggc
     1981 gtggccaaac acgtcgggtg gcatctcgaa gaggcggcgg taggcggcgg ctcagatgga
     2041 aacttcaccg ccggaattgg catcccgacg ctcgatggta tgggagggat gggcgacggg
     2101 gcgcacgccg ttcacgaaca catcgtgatc tcagaactgc cgcgccgcat gctgctgctg
     2161 gcgggtatga tcgaaaccat ctgacgcgag acacgatcgc atgctgaagt ttatggttgt
     2221 gctgtaccgg cggcctgaat tgactcccgc agagtttcgc cggcatctcg agcaagtcca
     2281 cggacccctg gcgaaagctc ttcccgggct gcgacgatac atccagaact atccctgcac
     2341 cgatgccaaa cgaaaatcgc ccggatggga tgccatcgtc gagctctact tcgacgactg
     2401 ggcggcaatg gaggcagcgt gggccagccc gcagggcgct gcttctgacg ctgatctgcc
     2461 tgccttcacg gatctgaccc gcaccacgtg gtctgccgtg gaagaagtca cggttgcgcc
     2521 gtgaagacca ggaagtaatc catctggtct ttcttgacga agtcgtactg ggccagcaga
     2581 tcgtagccgg cttcttttgc ctcgcgaatc acgacgtcgc ggccgacgcc gtgattctct
     2641 ccgttcccgt tgcgatcgat gactcccacc ttggccccgg ggcgcagcgc accccgcagc
     2701 ttccgcaaaa gcacgaccgg ttgcgcgacc tcgtgatagg tcttcagcag cagcaccgca
     2761 tccaccgagc gggcgggcag aagcgggtcg tcgggctcgc tcaggatcgt ctttacgttt
     2821 cccagctgtt ccttgtttga tccgcttgtc gatgtagcgg atcgcttcgg gattgatgtc
     2881 aacagcgtag acagaacctg ccgcgccgat gcgccgcgcc gcgcgcaccg agaaccagcc
     2941 cgaacccgct cctatgtcgg cgacactctt tcccggtgcg atgctcagga tgtccatgac
     3001 gcggctgatt tgcagacggt cgtcgcgccc gggagattcg aagaccgaga ggtctccggt
     3061 gtaaggctcg ctggttttgc gttctgtgcc agtcgctgag gtgggagctt cagtggtgct
     3121 ttgagcgcga gtcagggtga aaggatggcc aacgagcagg aggaggaccg gcaataggag
     3181 cagaagaaga acttttcgca ttacggggca cctccgggtc tgggagcacc tcttcgactg
     3241 accagcacct tcagcaatag acttgtccgg acggttcttg tgagcattcc cgagcgagtt
     3301 gaacacgaat gggtgagtag tgatgagttc gtgcccgcgc ccgtttttga ctcaccggcc
     3361 tgagtggcct agaattttac tggagtcgct gtcggagcgc ataacagccg ggttacggag
     3421 gagttgtggg tgagttttcc atctggcact ggttgctggt catagcgatc gcgctgctcc
     3481 tgtttggccc gtccaaactc ggatcgctgg gcaagggcct gggtgaaggc atccgcaatt
     3541 tcaggacggc aatgaaagag ggcgcagaag agaagaagga cgagaagccc gaagagaaga
     3601 agtcctagcg aaccgactcc ggtagttggc gacggtagct gtttccacaa actcctggct
     3661 acgccttttc cgcatcacct gaacgttcgc gcaggatgcg cagaaccagc gccaccggca
     3721 atcccaccac attggaatag tcgccttcga tgcggggaat gaagcgcgcc gccattccct
     3781 gaattgcgta ggctccggct ttatccatcg gctcgccggt cgccacatat ccgcggatgt
     3841 cctcttcaga gagctcgttc atggtcacca gcgtcgtttc ggatgccgtc gtaatgtgac
     3901 tcccgcctga tccttgtctt cctggcccgg cttccaccag gcataccccg gtgatcacgc
     3961 ggtgcacacg ccccgaaagc agtcgcagca tgcgggccgc gtcttcaccg tcgacaggct
     4021 tgccgaggat ggtttcatca accgctacga cggtgtctgc gcccagcacg cgatcatgcg
     4081 gcctggccgc gaacacggcg agagccttct cgcgggcgag acgctcggcg caatcccggg
     4141 gggattcgcc ggcaaacggg gtttcatcga cattcgccgg ttcgacgctg aatggaatgc
     4201 cggcgctgcg caagagttcc tgacgtcgcg gcgaggccga agcgagaacg agcatgccag
     4261 attctacagg gtgctccggt cacgacgaac ggatcagctc aatccggttc ccgaacggat
     4321 cggggatacg gcagcgccgc acgccgggaa tgctgtcgtc ttccctggtt tccacgcctg
     4381 ccgcccgcag gcgtgcagtc aaagcatcgt agtcgctgca gagcagggca ggatgggcct
     4441 tccgggctgg tcgaaaatcc ggctcaaccc ccagatgaat ttgcacggtc ccgctttcaa
     4501 accagcagcc gccacgcttg gccagttctg gtggcttcgc gatttcattc atgcccagca
     4561 ggtcgcaata gaaacgccgt gcggcgtcct ctccgccggg cggcatggcg agttgtacat
     4621 gatcgatggc ggcgaacgga ggaaggggca tggaacgatc cagcacagga gaattcgaca
     4681 gatggacatc ttaactgacc gacccggatc gcccattcct cgggcctttc aacggattcc
     4741 cactaacccc cgcaccgatt cgatcagctt ggcggaatcg tagccgatcc cttctttgaa
     4801 aacggtttcg acgttttcga tatcgtcgat tttctttgaa ggatcaccct tgatcacaac
     4861 cagatcagcc tgcttgccgg ccgcgatggt tccgatacgg tcgagttcgc ccaggtactg
     4921 cgcaccgttg aaagtcgcga tgtgaatcgc ttccagtggc gtgaatcctg cttcgaccag
     4981 cagttcgact tccctctggt cgccgaagcc ggccaaatct ccgcccatgc ccgtcggatc
     5041 ttctcccgcg atcagcaatc ctcccgcctg agcgaaggca tgttcaaatt cctcttcctg
     5101 ttcgaacatc ctgcgccacg gggcctgccg cacttctcct gtcttgggat ccttgatcgg
     5161 caagtctgcc tgtactcggt tgacgaggaa ctggttgcgc gcttcttccg acatggcgtc
     5221 caaaacgcgc tgctgcagcg gagtcttgtc gggcacgaaa agctcaaaga ccggcaaggt
     5281 tgacgttacc gccacatgat gagcgacgag atcgcgaatc atttcctgga tgggcgcatc
     5341 gctgacgcgc atccggcccg tttcaaccgc cactcctgcc atcgtagcca tggagtcgat
     5401 cgggcattgg tcgggtttct tcccggaata gaattccgta tccaccgtga tcccgtgctc
     5461 gagatcgtca atgcctagcg cggccgcctc gcgaaatccg atcgagcata gatgtcccgt
     5521 cactttgagt ccgcgtttgt gcgcggcatc gatcgcggcc ttgagcacgg cgcgcgtgat
     5581 gaagttgtag gctttgaagt tgtccacccc gcggtcggcc cagaaagtga cggtagcgac
     5641 cgcttcttcc ggagtattca gcgccgtcat ctgcggcgcc cagctccccg gcccttccag
     5701 gtaaggaccg gtcacgtgga tgcgcggccc aatcgattgg ccgctgtcaa tctgcttttt
     5761 gagcgcaagg tccatgtagg gttcaatact gcccgcggtc cgaatggtgg tcactccacc
     5821 ggccaggtac aagcgcggga aactgatcgc catttcgtga aacactgcgt cgcccgccgg
     5881 atagaacatg tgctcgtgca tgccgaccaa tccgggaatc acactgtacc cgtgcaggtc
     5941 gagcacctgc gcatccttgg gaatgctggc cgatgaagcg tcgctcaccg actcgatctt
     6001 tcccttgtta agaatcacgg tctgatcttc gcgcgcgggg gcgcccgttc cgtcgatcac
     6061 gcgcacatgg gttaaggcaa cgaccggagc gtcaatcttg acgaactggc gcacttcggg
     6121 agacagggtc tgcgcggcgg ccagagagga gcccagcagg accgaaatca ttattcgacg
     6181 catgattcct ccaaaaagct cgccggcatc ttcgtatcag aaccaggttg cagcgcggta
     6241 cttgcgatat tcgtcgccaa attttgcttc cagcacttgc gactccttgc gcgcacgcca
     6301 aagctgcaac ggaatgataa gaacaaatac cagcagccag atcggtcggc cgagcacaag
     6361 aataagcccc gcgatcacgc atgaaccgaa ggtgtagatc ggattccgta tcctggaata
     6421 gagtcccccg gtgacaagtt gtttcgcttg tgcggtgacg gtgagcgaac ggccaagctg
     6481 aaagcgggcg accgtccaga gagtgaaacc ggcggccagg aggcacgcgc caactatttg
     6541 cagcggagac caggccgcag gtgggtgctt ccagatgatt gcgccaaggg ctacaccgac
     6601 gatcagcgtg agcgcaacgt tgggtccgat c
//
LOCUS       DASPUL010000119         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_976496, whole genome shotgun sequence.
ACCESSION   DASPUL010000119 DASPUL010000000
VERSION     DASPUL010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976496"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VEJ00_03015"
     CDS             complement(<1..281)
                     /locus_tag="VEJ00_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXX00158.1"
                     /translation="MKILLVEDSKTIRRENESALHHAGYEVICAEDGESALKLARERK
                     PDLILLDLILPKISGPEVLKRLKREPATAGIPVVVLSSLTEKNKQKLIEA"
     gene            complement(408..1226)
                     /locus_tag="VEJ00_03020"
     CDS             complement(408..1226)
                     /locus_tag="VEJ00_03020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXX00159.1"
                     /translation="MQKSLKTLFLLSAGVMLASPALAQQTATTPASQPTTQSPAKTQA
                     GTAAKKAPATARSAPAVTLKTQKEKASYALGMKIGTDLHRQGVTEAVDPAIVARGLKD
                     ALVGGKTLMTEDEARTALAQLRTDVQQKTEAKAQQEGAANRKTGEAFLAANKSKPGVV
                     TLPSGLQYKILTQGNGPKPTASDTVSCNYRGTLIEGKEFDSSAKHGGPASFPVGGVIK
                     GWTEALQLMPVGSKWQLFIPPDLAYGERGAGGDIGPDETLIFEVELLSIEPPKK"
     gene            complement(1345..>3300)
                     /locus_tag="VEJ00_03025"
     CDS             complement(1345..>3300)
                     /locus_tag="VEJ00_03025"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00160.1"
                     /translation="SRASAPRTSLKWTIPGAVVLVLALVAGAIYLRRKPVVQLTDKDQ
                     LILADFNNQTGDPVFDSTLKEALAIQLEQSPLIQLVSDAELHDSLQYLGQPKNQHITP
                     ELAQQIGQRLGVKAYLAGTITPIGQAYVISINAVNCATGDVFGREQVTAPDKSAVLAA
                     VSSAATAMRARLGESLASIQKLSTPYLSSVTTASLQAFHAFSLGEDEHRMGRDFPEAA
                     SFYEQAVQLDPSFAMGWARLGVVYGNSGSSSKAQEYMKKAYDLRERVTERERMYIEAQ
                     HALQGYDLPKALESYELYASTYPRDANALNNLGSVLALEGNNEQAATAFVKTWEAAKW
                     DNTAATNAAGTYLALDRLADAERYQKEAAEQGGSENITYHINAALYGFMGEHSDWEKE
                     VQWAQPRPGGFQIEGQAANINFFLGKTHTAAQQWEHAAQRASQQHLPDTAGTYYALEA
                     THQALASDCTAARDASHRALALDRSEATVPDAALALALCGDTGPALQEMQRVASAEPT
                     NTLVNEVYLPEVKAAIALALHHPQQVAELLTPVAPYVMASSSPVILGRASLALGQWQQ
                     AVSDFAPGVKYRGVGLEEGSIGNLQGPDYSMCLLGTAQAQAQFDKAAAIKSYQQLLDI
                     WKNADADFIPAQEARRELTALTAPGKN"
BASE COUNT          567 a         1026 c          988 g          719 t
ORIGIN      
        1 gcctcgatca gcttctgctt gttcttttct gtgaggctgc tcaggactac aaccggaatt
       61 ccagccgtgg ccggctcgcg cttcaggcgt ttcagcactt cgggcccgct gatcttgggc
      121 aaaatcaggt cgagcaggat caggtccggc ttgcgctctc gggccagctt caaggccgac
      181 tctccgtctt cagcgcagat cacctcatat cccgcatggt gcagagccga ctcattttcg
      241 cggcggatcg tcttgctgtc ctcaactaag agaattttca ccttgccgtt ccctcgctgc
      301 ccttaacatc ggcaatcgag gggtcgcgca ttaattcagc acacaaacaa agaaaaccgc
      361 ttgcacgagg caagcggttt tgtcggaaac gcaggatcca gcgagtacta ttttttcggc
      421 ggctcaatcg aaagcagctc tacttcaaaa atcaaggtct cgtccgggcc gatatcgccg
      481 cctgcgccac gttccccata ggcgaggtcc ggcgggatga acaactgcca cttggatccg
      541 accggcatca actgcagcgc ctcggtccag cccttgatga ctccgcccac gggaaacgat
      601 gccgggccac cgtgcttggc ggaactgtcg aattctttgc cctcaatcag cgtcccgcga
      661 tagttgcacg agaccgtatc gctcgccgtg ggcttcggcc cgttgccctg cgtcagaatt
      721 ttgtattgca gaccgctggg cagtgtaacg actcccggct tcgatttgtt ggccgccagg
      781 aatgcctcgc cggtcttccg gttggccgct ccttcttgct gcgccttggc ttccgttttc
      841 tgctggacat cggttcgcag ctgggcgagc gcggttcgag cctcgtcttc cgtcatgagg
      901 gttttgccgc ccaccaacgc gtctttcaat cctcgagcca cgattgccgg atcgaccgcc
      961 tcggttacgc cctgccggtg cagatcggtg ccgatcttca tacccaaagc gtagctggct
     1021 ttttctttct gcgtcttgag cgtcaccgcg ggtgcgcttc tggcggtggc cggcgccttc
     1081 ttggctgccg ttcccgcttg cgttttggcc ggggactggg ttgttggctg ggaggctggg
     1141 gttgtcgcgg tttgctgcgc aagagcaggg ctggccaaca tcactcccgc tgacaacaga
     1201 aacagcgttt tgagggattt ctgcatagac ttgcattgtc ccattaggcg cgagaaatga
     1261 gcaaacggga gccgtcagaa ggagtggtta gtcggagggc cgagtactga tcactcgtca
     1321 ccgaccactg atcactgggc ctcttcagtt tttcccaggt gccgtaagcg ccgtcagttc
     1381 gcggcgggct tcctgggcag gaatgaaatc cgcgtcggcg ttcttccaga tatcgagcag
     1441 ttgctgatag ctcttgatcg cggcggcctt gtcgaactgc gcctgcgcct gcgctgttcc
     1501 cagcaggcac atggaatagt cgggtccctg caggttgccg atcgatccct cttcaaggcc
     1561 gacgccgcga tatttcaccc cgggtgcaaa gtcgctgact gcctgctgcc attggcccag
     1621 cgccagagaa gcccgcccca gaatcactgg cgaactggaa gccatcacgt agggcgcgac
     1681 gggtgtaagc aactcagcca cctgttgtgg gtgatgcagt gcgagcgcga tggcagcctt
     1741 cacttccggc aggtagacct cgttcacgag cgtgttggtg ggttccgccg aagcgacccg
     1801 ttgcatttcc tgcaaagccg gaccggtgtc gccgcagagc gccagggcaa gcgcagcgtc
     1861 cggcacagtc gcttccgagc ggtcgagcgc cagcgcgcga tgcgaagcat cgcgggctgc
     1921 cgtgcaatcg gaggccagcg cctgatgggt cgcctccagt gcgtagtagg tcccggccgt
     1981 atccggcaga tgctgctgag acgcccgttg cgcggcgtgc tcccattgct gcgctgccgt
     2041 gtgcgtcttc cccaggaaga agttgatgtt agccgcctgg ccctcaatct gaaagccgcc
     2101 cggccgaggt tgagcccatt gcacttcctt ttcccaatcg gaatgttcgc ccatgaagcc
     2161 atataaagcg gcgttgatgt gataggtgat gttctcgctt ccgccctgct ccgccgcttc
     2221 tttctgatag cgctccgcgt cagccagacg gtcgagcgcg aggtaggtcc cggccgcgtt
     2281 ggttgcggct gtgttgtccc acttggcagc ttcccaggtt ttcacgaacg cagtcgccgc
     2341 ctgctcgtta ttcccttcca gggcaagaac cgagcccaga ttgttcaacg cattggcgtc
     2401 gcggggataa gtcgaagcat agagctcgta ggattccagg gccttcggca aatcgtaccc
     2461 ctgcaaggca tgctgcgctt cgatgtacat ccgttcgcgc tccgtgacgc gctcgcgcag
     2521 gtcataggcc tttttcatat attcttgcgc cttggaggat gaacccgagt ttccgtagac
     2581 gacgcccagc cgcgcccagc ccatggcgaa agagggatcg agctgcacgg cttgttcgta
     2641 gaaagaagcc gcttccggaa aatcgcggcc cattcggtgt tcgtcttcgc ccaaggagaa
     2701 agcatgaaac gcctgcagcg acgccgtggt gacgctgctc aggtagggag tcgacagctt
     2761 ctgaatcgac gccagcgact cgcccagccg cgctcgcatc gccgttgcgg cgctggaaac
     2821 cgcagccagc accgccgact tgtcgggcgc ggtgacctgt tcgcgtccaa acacgtcgcc
     2881 ggtggcgcag ttcaccgcgt tgatggagat gacgtatgcc tgtccgatcg gtgtaatcgt
     2941 tcccgccagg taggccttca cgccaagccg ctgcccgatc tgctgcgcca gctcgggcgt
     3001 aatgtgctga ttcttgggct gtcccagata ctgcaggctg tcgtgcagct cggcgtcgct
     3061 gaccagctgg atcagcggcg actgctccag ctggatcgcc agcgcttcct tcagagtgga
     3121 gtcaaatacg gggtcgcccg tctgattatt gaagtcggcc aggatgagct ggtctttgtc
     3181 ggtcagctgc acgaccggtt tcctgcgcaa gtagatcgcg ccggcaacga gcgccaacac
     3241 cagcacaacc gccccgggaa tcgtccactt gagcgaggtg cgtggagcgc tcgccctcga
//
LOCUS       DASPUL010000120        10896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_20809, whole genome shotgun sequence.
ACCESSION   DASPUL010000120 DASPUL010000000
VERSION     DASPUL010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10896)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10896
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20809"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..2241
                     /locus_tag="VEJ00_03030"
     CDS             274..2241
                     /locus_tag="VEJ00_03030"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXX00161.1"
                     /translation="MTPRTQKITEVLLGPRLDPFSTQTRKHAALVAVLAWVGLGADAL
                     SSSCFGPEQAFLALGTHTHFGLYLAGATALTVFIIGVAYNQVIELFPSGGGGYKVATN
                     LIGPYTGLVSGAALIVDYMLTIATSMASGVDAMFSLLPLGAQPFKLATDVGLVLLLMV
                     LNLRGMKESIQVLLPIFLGFFVIHALLIAYGVAFHAEALPDLIPDTLKETRGLSREMG
                     WVFALSLFLRAYSLGGGTYTGIEAVSNNVQSLAEPRVKTGKWTMFYMAVSLSLTAGGF
                     ILLYLLWNVKPEEGQTLNAVTFRDIIHSWGLASPIVESGALLLVLALEGALLFVGANT
                     GFLGGPAVLANMAADSWVPHQYRYLSTRLVTQNGILLMGLASVAILLISGGQVSMLVV
                     LYSINVFLTFSLSLAGLCIYWWQARRQDKRWSHRIVLSLCGLTVTFSILVITLVEKFA
                     EGAWLTALITGLVIAACLVNHRHYLKIKRKIHAADQALSWLEYPDVDNPPLLDPAGHT
                     AVLVVGSSRSGGVHALQWVRREFPGSFKNFIFMNVRTVDSQCYSGDQELEKQRELANT
                     ALTYFVNFCHHEGLASKSYLAFGTDPIAELIRLAEKVKQDFPNSVFFTSKLIFERDNW
                     YIRQLHSEAALQMLRQLHLRNMPMVILPMRL"
     gene            2564..3886
                     /locus_tag="VEJ00_03035"
     CDS             2564..3886
                     /locus_tag="VEJ00_03035"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HXX00162.1"
                     /translation="MKRIPRLFRSLLYTLRGGFLIRPLVIAVLLGVVGAVLSEAEEAI
                     PAVGAWLPATLFPSRQDPQVAQAILSGIATSIMTVVSIVFAILLMTLTLASTQFSPRI
                     LVSFVRDRTTQWTLGVFLGTFSYCMAALPAARSLPHPFVPVATTTGAMLLALVCVGWL
                     IFFINHISQAISVNHIVDRIARETELIIDDFMPDPRAPFMRSEGIELHGSKDGLPVLS
                     QLSGYIRYLDIRQLFQLAKDYRVHIRVVRRVGHFVPAGVPLLTASPGERITAERSERL
                     LAAFDIGPTRTMQQDVEFGVIQIVDIALRAISPAVNDPSTAISCIDQLSRILIRWLSR
                     ASAASYFYSPPHVLRLIVPWITLDGLLDTAFEQIRHYAAGDVAVSLRLLRAFTDIAST
                     TDRLDVHHNLLERAQRVIAGCTGHLTETDLAALQQRIAPLKLRVSMNA"
     gene            complement(3978..4151)
                     /locus_tag="VEJ00_03040"
     CDS             complement(3978..4151)
                     /locus_tag="VEJ00_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00163.1"
                     /translation="MDKQMVQLDGDVRGYRVSTTAPAVARPALDLFYFMNSFGVLTQQ
                     WRAGLIAVGPPSL"
     gene            4303..4848
                     /locus_tag="VEJ00_03045"
     CDS             4303..4848
                     /locus_tag="VEJ00_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00164.1"
                     /translation="MQSAGEDVMTMSAAEFIGASATKQDRSRMRELTALAYIVVFDAL
                     KLDYGADEARSEKERKQCSARAGAAADYLFGREPSSRAVEALDLAREQEAARNWLLRN
                     ARMRELVLESLRVLNVLNEHDHTRTGLVGEGLVMAYGQGSFRAPDVATYDALVHDAIY
                     SLPLHAQQGIFYAWAKTQTCH"
     gene            5075..6892
                     /gene="kdpA"
                     /locus_tag="VEJ00_03050"
     CDS             5075..6892
                     /gene="kdpA"
                     /locus_tag="VEJ00_03050"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HXX00165.1"
                     /translation="MSANGYFQLLAYGVILLVLVRPLGAYMARIFDGQPAFLNRVAAP
                     LEQFLYRLCRVDPTREMRWTQYALALLAFNLLGAIILYALQRLQSFLPLNPQGFSGVP
                     QDLAFNTAAGFVTTADWQSYAGEASLSYLVQMAGLSVQNFMAAASGMAVLLPLARGFA
                     RQNADVVGNFWVDLTRGTVYILLPLALVFALVLVSQGVVQTFSAYRTTPLVESVEYDN
                     PKLDAAGQPIKDDKADPVTEKATKKEQVIALGPAASQVSIMELGSNGGGFFNANSAHP
                     FENPTPLSNFLELLAILLIPAALCYTFGKMVGDTRQGWAILAAMALVFVVLLALCVGA
                     EQSGNPTLTKLGVDQTASPLQPGGNMEGKEARFGIVSSTLWATATTASGNGSVNSMLD
                     SLTPVGGLVPMALIQLGEVIFGGVGSGLYGMLMFAIVTVFVAGLMIGRTPEYLGKTVE
                     AFEMKMASIAVLIPSAVALLATAIAVSVDAGKVGVANPGAHGFSEILYAFCSAANNNG
                     SAFAGLSTNTPFYNTALGLVILVGRFWVAIPVLALASSLAAKKKRAASAGTLPTHTPL
                     FIAALIGTVLIVGGLTFFPALALGPIVEHLSPLRAGLVR"
     gene            7098..7187
                     /locus_tag="VEJ00_03055"
     CDS             7098..7187
                     /locus_tag="VEJ00_03055"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF021144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HXX00166.1"
                     /translation="MTWLYALSGIVALVITIYLVIALLFPEKF"
     gene            7188..8993
                     /gene="kdpA"
                     /locus_tag="VEJ00_03060"
     CDS             7188..8993
                     /gene="kdpA"
                     /locus_tag="VEJ00_03060"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HXX00167.1"
                     /translation="MSANGYLQLIVYVVVLLALAKPVGSYMARIFEGRPAVLNTVGGP
                     LERFLYRVCGVDAAKEMRWTQYALAMLAFNLLGGLAVYGLQRLQAYLPLNPPNFPAVT
                     PDLSFNTAASFITNTNWQSYGGESTMSYLTQMAALTVQNFLSAATGIAVLLPLIRGFA
                     RQKMDTVGNFWVDLTRTTLYILLPLALVFALVLVSQGVVQTFSAYPTTPLVESVEYDN
                     PKLDAAGQPIKDDKGNPVTEKATQKEQSIAVGPAASQIAIKQLGTNGGGFFNVNSAHP
                     FENPTPLSNFLEVLAILLISAGLCYTFGKMVGDTRQGWAILAAMTLVFVVLLAVCVGF
                     EQAGNPALTKLGVDQTTTPMQSGANMEGKEVRFGIANSALWATATTSASNGSVNAMHD
                     SFTPLGGLVPMWLIQLGEVIFGGVGSGLYGMLMFAIVAVFVAGLMIGRTPEYLGKKIE
                     AFEMKMAAIAVLIPLIMALGLTALGVLTDAGKAGIANPGAHGFSEILYAFSSAAGNNG
                     SAFAGLNANTPFYNTALGFDMLFSRYWLTIPVLAIAGALVTKKKVPVSLGTLPTHTPL
                     FIAMLVGVVIIIGALTFFPALALGPIVEHLAMYGG"
     gene            9034..>10896
                     /gene="kdpB"
                     /locus_tag="VEJ00_03065"
     CDS             9034..>10896
                     /gene="kdpB"
                     /locus_tag="VEJ00_03065"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HXX00168.1"
                     /translation="MSTQVGAPALPPTPALPRTLVTKSIFQQALVDSVVKLLPQYQWR
                     NPVMFVVYVGSILTTFLYFQALGGKGEAPAGFILAVSLWLWFTVLFANFAEAVAEGRS
                     KAQAAALRAVKREVGAKKLTAAKRDAPFNWVPSTALRKGDAFLVEAGEVVPADGEVIE
                     GVASVDESAITGESAPVIRESGGDFCSVTGGTRVLSDWLVVRVTVDPGESFLDRMISM
                     VETARRQKTPNEIALWILLVKLTLVFLLATVTLLPFSLYSVEVAKAGNPITITVLVAL
                     LVCLIPTTIGGLLSAIGLAGIGRMLRANVIATSGRAVEAAGDVDVLLLDKTGTITLGN
                     RQATAFRPASGVTEQELADAAQLASLADETPEGRSIVVLAKQKFALRERDVASLGATF
                     VPFSAQTRMSGVDMGERQIRKGAADAIRTWVQSKGGSLPTEVVGFVETMARQGSTPLV
                     VADGAKVLGAIELKDVVKGGIKERFAQLRRMGIRTVMITGDNRLTAASIAAEAGVDDF
                     LAEATPEAKLKLIRDNQAGGRLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNM
                     VDLDSNPTKLLEIVEIGKQLLITRGALTTFSIANDVAKYFAIIPAAFATTYPQLI"
BASE COUNT         1994 a         3342 c         3259 g         2301 t
ORIGIN      
        1 gatcggagta ggaacgaccg actgctgagt agtagttcgc cccatagcaa aggcgagacg
       61 agcgagctta tctcatcgcg ctctgatcag agtccgacgt ggtcaacgtc caatctgacg
      121 aacatgatcg gaaaactgga agtcaatcgt tgagccgtac tagactagtc ggctcgtcca
      181 atcgacacgc aagctatcgg gacggccaac ccgaattggg ggtaatagaa tcattcgatc
      241 tgacgcactc gcccgaatca accgaggaag agagtgacgc ctcgcacgca aaaaatcaca
      301 gaagttctgc ttggcccgcg gcttgatccg ttcagcacgc aaacccgcaa gcacgccgcg
      361 ctagtggcgg ttctcgcctg ggtagggctt ggtgcggatg cgctctcctc gtcctgcttt
      421 ggcccggaac aggcctttct cgcgctcggc actcataccc atttcggcct gtacctggcc
      481 ggggcgaccg ctttgacggt gttcatcatc ggggttgcat acaaccaggt tatcgagctg
      541 tttccctcag gtgggggcgg ctacaaggtc gccaccaacc tgatcggccc ctataccggc
      601 ctcgtctccg gcgcagcgtt gatcgtcgac tacatgttga cgatcgccac gtcgatggcc
      661 agcggggtgg acgccatgtt cagcttgctt cccctgggcg cccagccatt caagctagcg
      721 acggacgtcg gcctggtgtt gctgctgatg gtgctcaatc tgcgcgggat gaaggagtcc
      781 attcaggtcc tgctgccgat tttcctcgga ttctttgtca tccacgcctt gctcattgct
      841 tacggcgtcg ctttccacgc cgaggccctt ccagacctga tcccagacac tctcaaggaa
      901 acccgcggcc tgtcccgaga aatgggatgg gtgttcgccc tttctctatt cctgcgcgcc
      961 tactctcttg gcggtggtac ctacacgggg atcgaggcgg tttccaacaa cgtgcagtcg
     1021 ctagccgagc cacgtgtcaa gaccggcaag tggacgatgt tctatatggc ggttagcttg
     1081 agtctcactg cgggaggatt catcctgctt tatctcctgt ggaacgtgaa accggaagaa
     1141 gggcagacgc tcaatgcagt gacctttcgc gacatcattc acagctgggg cttggctagt
     1201 cccatcgttg aaagtggagc acttctgctc gttctcgcgt tggaaggtgc gctgctgttc
     1261 gtaggcgcca acactggctt cctcggcggt ccggcggtgc tggccaacat ggccgcggac
     1321 tcctgggtgc cgcatcagta tcgctacctt tccacacgcc tggtcaccca gaacggcatc
     1381 ttgttgatgg ggctcgcttc ggtggccatt ctgcttataa gcggcggtca ggtctcgatg
     1441 ctcgtggtgc tctacagcat caacgtgttc ctgacgtttt ccctgtcgct cgcgggattg
     1501 tgcatttatt ggtggcaagc gcgccgccag gacaagagat ggtcgcatcg catcgtgctt
     1561 tccctgtgcg gcctgaccgt cacgttcagc atcctggtga ttaccctggt tgagaagttt
     1621 gccgagggcg catggctcac cgcgctgatc accggcttgg tcatagcagc ctgcctcgtc
     1681 aaccatcgac actacctcaa gatcaagcgg aaaatccacg cggcggacca ggcactctct
     1741 tggctggaat accccgacgt ggacaatcca cccctcctag atccggccgg acacacagcc
     1801 gtattggtgg tcggctccag ccgcagtggc ggggtgcacg ctttgcagtg ggtacggcgc
     1861 gagttcccgg ggagtttcaa gaacttcatt ttcatgaacg tgcgcacagt ggactcgcag
     1921 tgctacagcg gcgaccaaga gctggagaag cagcgagagc tggccaacac agcgctcacc
     1981 tacttcgtaa atttctgcca tcacgagggg ctggcctcga aatcctatct cgccttcggc
     2041 accgatccga tcgcagagct catcaggctt gcagagaagg tcaagcaaga ttttcccaac
     2101 agcgtcttct ttaccagtaa gctgatcttc gagcgcgaca actggtacat tcgccagctg
     2161 cacagcgagg cggccctgca aatgctgcgc caactccact tgcgcaacat gccgatggtg
     2221 attttgccga tgaggctgtg atacccctac tggcttccac gttgcgaccc tgaacacgcg
     2281 ggatgccgag ccggtcgaag cctcgcacat ggcagccacg tcctcacccc cgcgcgtctc
     2341 tacgtaaccg tcctggaccg taccgtttga tcacaggcgg gctcacgtgt cttcacccgc
     2401 ggcgcttggt cggatggtcg cgcaactggg ttctcacccc tggcgatagt ctgcaatgcc
     2461 acatgcaaga cgacgagcca tccattgcgc ctcgcagtct ggtgtactct ctcggcgggt
     2521 tctgatcatg gacccaagcc cctatgtatc tcgcccagct acgatgaagc gcataccccg
     2581 gctgttccga tcgttgctct atacgctgcg tggcggattc ctgatccggc ccctcgtcat
     2641 cgccgtgctc ctcggggttg taggtgcggt gctgtcggaa gcggaagaag cgatcccagc
     2701 agttggtgct tggcttccgg cgaccctgtt cccgtcacgg caagatcctc aggtagcgca
     2761 ggccattctg agcggcatcg cgacctcgat catgactgtc gtgtcgatcg tgttcgcgat
     2821 tctgctgatg acgctcactc ttgcatctac gcaattctct ccgagaatcc tggtgagctt
     2881 cgtgagggat cgcaccacgc agtggacgct gggagtgttc ttggggacat tttcttactg
     2941 catggccgcg ctgcccgcgg cccggtcgct tccccatccg ttcgtgccgg tcgcaaccac
     3001 taccggtgca atgcttttgg cgttggtgtg tgtcgggtgg ctaattttct tcattaatca
     3061 tatctctcag gcgattagcg tcaaccacat cgtcgaccgg atcgcgcgcg agaccgaact
     3121 gatcatcgat gacttcatgc cggatccgcg cgctccattc atgcgttcag agggcatcga
     3181 gctgcacggc agcaaggacg gactccccgt cctcagccag ctgtcaggct atatccgcta
     3241 cctggacatc aggcagctct ttcagctggc gaaggactac cgcgtccaca ttcgggtagt
     3301 gcgccgcgtt ggtcatttcg ttccagcggg ggtcccactg ctgacggcgt cgccgggtga
     3361 gcgcatcacc gcggagcgat cggagcgact tctagccgcc tttgacatcg ggcccacgcg
     3421 taccatgcag caggacgtcg agttcggcgt gatccagatt gtcgacatcg cgctcagggc
     3481 gatctcgccc gctgtgaacg atcccagcac cgcgattagc tgcatcgatc agctcagcag
     3541 aatattgatt cgatggttga gtcgcgcttc tgcggcttcc tatttttatt caccaccgca
     3601 cgtgctgcgg cttatcgtcc cctggatcac cctcgacggg ctgctcgaca ccgccttcga
     3661 acaaatccgt cattacgccg caggtgacgt ggcggtaagt ctcaggctgc tccgtgcatt
     3721 cactgacatt gcctcgacca cagatcggct cgatgttcac cacaatcttc tcgagcgcgc
     3781 ccagcgtgtc atcgctgggt gtacagggca tctcacagaa accgaccttg ccgctctgca
     3841 acagcggatc gcgccactca agttacgtgt ttcgatgaac gcatagcggc gcaatacaac
     3901 gaggatgctc cacacaagaa tgcctctgct tagacacgat tgatgcgctg ctgagtcctg
     3961 gtgcttggga tggaaggcta gagggagggc gggccgaccg cgatcagtcc ggcgcgccac
     4021 tgctgagtaa gtacgccaaa gctattcatg aaataaaaca ggtccagcgc tggccgggca
     4081 acagcaggtg ccgtagtcga aactcgatat cccctcacgt cgccatccaa ttgaaccatc
     4141 tgtttgtcca tcaaaaggcc tcgagtctgg ccggcctcgc gcatctttac agatttttta
     4201 tagctgccga cgaagtcttt gcaccattcc tgcgtgcgta agcgtaaatt gcatcttgtg
     4261 gtcgtgggtt acgattgagc agcctaggaa acagcgtagc gcatgcaaag cgcaggagag
     4321 gatgtcatga ccatgtcagc agcagagttc atcggtgcga gcgcgacgaa gcaggatcgc
     4381 tcgcgaatgc gagaactgac cgcactcgcc tacatcgtgg tgtttgacgc gctcaagttg
     4441 gattacggcg cggacgaagc tcggtctgag aaggaacgta agcagtgtag cgcgcgagcc
     4501 ggcgccgcag ccgactacct gtttggtaga gagccgagca gtcgcgcagt cgaagcgctt
     4561 gatcttgctc gagagcagga ggctgctaga aactggttgc tgcgcaacgc tcgcatgcgg
     4621 gagcttgttc tggagagcct tcgcgttctc aacgtgctga acgaacacga tcacacccgg
     4681 acaggcctcg tgggcgaagg ccttgtcatg gcctatggtc aaggctcttt ccgggcgcct
     4741 gacgtggcca cttatgatgc tttggttcat gacgccatct atagtctgcc actgcacgcc
     4801 caacagggca tcttctacgc atgggcgaaa acccaaacat gccattaggg ccgccccgta
     4861 ggacggcaga tcagtcgaag ttcaagtcac gaggttgacc ggcaaggttg gccgatcaaa
     4921 ctttggtctc cctcgggagc accgccaagg atgtgatcgc ggaactacct ctctcacgca
     4981 agctgcaggt gaagtgctct atcacatgct acgcaagcac ccggcacgat tagaggggta
     5041 ttgagatcga agcgcttgcc cggaaggatg cccgatgagc gctaatggct actttcagct
     5101 gctggcatac ggggtgatcc tgctggtgct ggtgagaccg ctcggcgcct acatggcccg
     5161 catcttcgac ggccagcccg cgttcctcaa tcgcgtcgca gcgccgctgg aacagttcct
     5221 gtatcgactc tgccgcgtcg atccaacgcg ggagatgcgc tggacccaat atgctctggc
     5281 gctgctggca ttcaatctcc tgggcgcgat catcctttac gcgctgcaac gtctgcagtc
     5341 gtttcttccc ctcaatccgc agggattctc gggagtcccc caggatcttg cattcaatac
     5401 cgcagccggc ttcgttacca ccgcggactg gcaaagctat gcgggtgagg catcactcag
     5461 ttacctagtg cagatggccg gattgtcggt acagaacttc atggcggcgg cgtcaggaat
     5521 ggcggtgttg ctgccgcttg cgcgcggttt cgctcggcaa aacgccgatg tggtcggcaa
     5581 cttctgggtc gatctcaccc gcggcacggt ctatatccta ttgccgttgg ccctggtgtt
     5641 tgccctagtc ttggtctcgc agggggtggt gcagaccttc tccgcctatc ggaccacacc
     5701 tctggtagag tcggtcgaat atgacaaccc caagctcgat gctgcggggc agcccatcaa
     5761 ggatgacaaa gccgaccccg taaccgagaa ggcgaccaag aaagaacagg tcatcgcact
     5821 gggtcctgca gcctcgcagg tctccatcat ggagctaggg agcaatggag gaggcttttt
     5881 caatgccaac tccgcgcatc cgttcgagaa ccctacgccg ctgtccaatt tcctcgagct
     5941 gctcgccatt ctgctgatcc ccgccgccct gtgttacacc ttcggcaaga tggtgggaga
     6001 cacgcgccag ggctgggcca tcctggcggc gatggcgctg gtgttcgtcg ttctgctcgc
     6061 actgtgcgtc ggagctgagc agtcgggtaa tcctaccctg accaagctcg gtgtcgatca
     6121 gacggccagc ccgctgcagc cgggtggaaa catggaaggg aaggaggcgc gcttcgggat
     6181 cgtgagctca acgctttggg caacggcgac aacggcctcc gggaacgggt ctgttaattc
     6241 catgctcgac tcgttgacgc ccgttggggg attagtgcca atggcgctga tacagctcgg
     6301 ggaagtcatc ttcggcggcg taggctccgg gctgtacggg atgctgatgt ttgccattgt
     6361 gacggtcttt gtagcggggt tgatgatcgg gcgcacgcct gagtatctgg gcaagactgt
     6421 cgaggccttc gagatgaaga tggcctcgat cgcagtcctg attccctcag cggttgcact
     6481 gcttgctacc gccatcgcag tctcggttga cgcgggcaag gtcggagtcg cgaacccagg
     6541 agcgcatggg ttctcggaga tcctatatgc cttctgctcg gccgcaaaca acaacggcag
     6601 tgcctttgcg ggtctgtcga ctaacacgcc cttttacaac actgcgttag ggctcgtgat
     6661 tctggttggc cggttctggg ttgccatccc tgtgctcgcc ctggccagct cgttggcggc
     6721 gaagaagaag cgtgcggcga gtgctggcac gctgcccacc cacacgcctt tgttcatcgc
     6781 cgcactgata ggtaccgtgt tgattgttgg cggccttacg ttcttcccgg cgcttgcgct
     6841 tggcccaatc gtcgagcatc tgagtccgtt gagagctgga ctagtacgct gagaggtaat
     6901 taagcggaaa gatttttatg ccatttttat acggtcggtc cggttctttg cactgttttt
     6961 attcgagctt gtctagcatg cactagttac gcagaggagg cgacaatgat ggacgcggtg
     7021 tacctattga tcgtgattgg gttcttcgta ctctcggccg cggtcgtcta tggctgcgag
     7081 cgcttgagga gaccctcatg acctggttat acgccttgag cggcatcgtg gcgctcgtca
     7141 tcacgatcta tctcgtgatc gctctgctgt ttccggagaa gttctaaatg agtgccaacg
     7201 gctatctgca actaatcgtg tatgtggtcg tcttgctggc gctcgcgaag cccgtgggct
     7261 catacatggc gcgcatcttc gagggccggc cagccgtgct caacaccgtg ggcgggccgc
     7321 tcgagcggtt cctttatcgg gtgtgcggtg ttgacgctgc caaggagatg cgctggacgc
     7381 agtatgcgtt ggccatgctc gctttcaacc tgctgggcgg gttggcagtt tatggtctgc
     7441 aaaggctgca ggcttatctg ccgctcaatc cgccgaactt ccctgcggtc acgcccgacc
     7501 tgtcgttcaa caccgctgcc agtttcatca ccaacaccaa ctggcagagc tacggcggcg
     7561 agagcacgat gagctatctt acgcagatgg ccgcgctcac ggtgcagaac ttcctgtctg
     7621 ccgcgacggg catcgccgtg ctgcttccgc tgattcgagg tttcgcgcgc cagaagatgg
     7681 atacggttgg caacttctgg gtcgacctca cccgcacgac gctctacatt ctcttgccgt
     7741 tggcgctggt gtttgccctg gtcctggtct cgcagggcgt ggtgcagacc ttctccgcct
     7801 atcccactac acctctggtc gaatcggtcg agtacgataa tcccaagctc gacgccgccg
     7861 ggcagccgat caaggacgac aagggcaacc cggtcacaga gaaagccacg caaaaggagc
     7921 agagcatcgc agtcgggccg gccgcatcgc agatcgcgat caagcagctc ggcaccaacg
     7981 gcggcgggtt cttcaacgtc aactcggcgc atccgttcga gaatccgact ccgctctcca
     8041 acttcctcga ggtgctcgct atcctgctga tctcggcggg cctgtgctac accttcggca
     8101 agatggtcgg cgacacgcgc cagggctggg cgatcctggc cgccatgacg ctcgtgttcg
     8161 tggtgctgct ggccgtgtgc gtgggcttcg agcaggccgg caatcccgcc ctcaccaaac
     8221 tgggcgtcga tcagaccacg accccgatgc agtcgggcgc caacatggaa ggcaaggagg
     8281 tgcgcttcgg catcgccaac tcggccctgt gggccaccgc aacgacctcg gcgtcgaacg
     8341 gctcggtcaa cgccatgcac gattcgttca ccccgctcgg gggtctggtg ccgatgtggc
     8401 tgatccagct gggcgaggtg atcttcgggg gcgtgggctc gggcctgtac ggcatgctga
     8461 tgttcgccat cgtggctgtg ttcgtagcgg ggcttatgat tgggcgcaca ccggagtacc
     8521 tgggcaagaa gatcgaggcc ttcgaaatga agatggccgc catcgccgtg ctgataccgc
     8581 tgatcatggc gctgggcttg accgcgctgg gcgtgctcac ggatgccggc aaggccggca
     8641 tcgccaaccc aggtgcgcac ggcttctccg agatcctgta cgccttttcc tcggccgcag
     8701 gcaacaacgg cagcgcattc gcaggtctga atgccaacac tccgttctac aacacggcgt
     8761 tgggcttcga catgctgttc tcgcgttact ggctgaccat cccggtgctc gccatcgccg
     8821 gcgcgctggt caccaagaag aaggtgccgg tgagcctggg cacgctcccg acgcacacac
     8881 ccctgttcat cgccatgctg gttggcgtcg tcatcatcat cggcgctctc acgttcttcc
     8941 cggcgttggc gctcggcccg attgtcgaac acctggcgat gtacggcgga tgacagcctt
     9001 gacatattgc gacagtttac ggagtcctct catatgagca cccaagttgg cgcacctgcg
     9061 ctgcctccta cccccgcgct gccgcgcacc ctggtgacca aaagcatctt ccagcaggcg
     9121 ctggtggact ccgtggtgaa gctgctgccg cagtatcagt ggcgcaatcc agtgatgttc
     9181 gtggtgtacg tcggcagcat cctcaccacg ttcctgtact tccaggcttt gggcggcaag
     9241 ggtgaagcac cggctgggtt catccttgcg gtcagcctct ggctgtggtt taccgtcctg
     9301 ttcgccaact tcgctgaggc ggtggccgaa ggccgcagca aggcgcaggc ggcagcgctg
     9361 cgcgcggtca agcgcgaagt cggggcgaag aagctcaccg cggccaagcg tgatgctcct
     9421 ttcaactggg tcccctccac ggcgcttcgc aagggcgacg cgttcctggt cgaagccggc
     9481 gaagtcgtcc cagccgacgg ggaagtaatc gagggcgtgg cctcggtcga cgaaagcgcg
     9541 atcaccggcg agtcggctcc agtgatccgc gagtcgggcg gagacttctg ctcggtcact
     9601 ggtggcactc gagtcttgtc cgactggctg gtcgtgcgcg tgaccgtcga tccgggcgag
     9661 tcttttctgg atcgcatgat ctcgatggtg gaaaccgccc gccggcagaa aacacccaac
     9721 gaaatcgcac tctggatcct cttggtcaag ctgacactgg tgttcctgct ggctacggtc
     9781 acgctgcttc cgttctcgct gtatagcgtg gaagtcgcca aagccggcaa cccgatcacc
     9841 atcaccgtgc tggtcgcatt gctggtctgc ctcatcccga ccacgattgg cgggctgctc
     9901 tcggccatcg ggttggccgg catcggccgc atgctgcgcg ccaacgtgat cgcaacgtca
     9961 gggcgcgcgg tcgaagcggc cggcgatgtc gatgtcctgc tgctggacaa gaccggcacc
    10021 atcacgctgg gcaaccggca ggcaacggcg tttcggcccg cctccggcgt cactgaacag
    10081 gagcttgccg acgcggcgca gctcgcgtca ctggccgatg agacgcccga aggccgcagc
    10141 atcgtagtgc tcgctaagca gaagttcgcg ctgcgcgaac gcgacgtggc ctcgcttggc
    10201 gccaccttcg tccccttctc ggcccagacc cgcatgagcg gcgtcgatat gggcgagcgg
    10261 cagatccgta aaggcgccgc cgacgccatc cgcacctggg ttcaatccaa agggggcagc
    10321 cttcccaccg aagtggtcgg gttcgtcgag accatggccc gacagggctc gactccgctc
    10381 gtggtcgccg acggcgccaa ggtgctcggc gcgatcgagc tgaaagacgt cgtcaagggc
    10441 ggcatcaagg agcgcttcgc gcagctgcgc cggatgggca tccgcaccgt catgatcacc
    10501 ggggacaatc gcttgaccgc cgcgtccatc gccgcagagg ccggcgtcga tgatttcctg
    10561 gcggaggcca cgccagaggc caagctcaag ctgatccgcg acaatcaggc cggcgggcgg
    10621 ctcgtcgcca tgaccggcga cgggaccaac gatgcgccag cactcgcgca ggcggatgtg
    10681 gccgtggcca tgaataccgg cacccaggca gcgaaggaag ccggcaacat ggtcgacctg
    10741 gattccaacc cgacgaagct gctcgagatc gtcgaaatcg gcaagcagct cctgatcacg
    10801 cgcggcgcgt tgaccacgtt ctcgatcgcc aacgacgtcg cgaagtactt cgccatcatt
    10861 cctgcggcat ttgccacgac gtaccctcag ctcatc
//
LOCUS       DASPUL010000121         3195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1387472, whole genome shotgun sequence.
ACCESSION   DASPUL010000121 DASPUL010000000
VERSION     DASPUL010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387472"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /gene="asnB"
                     /locus_tag="VEJ00_03070"
     CDS             complement(<1..1845)
                     /gene="asnB"
                     /locus_tag="VEJ00_03070"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXX00169.1"
                     /translation="MCGIAGLMNGGSAELLGRMNDIIAHRGPDDAGLKWFDDACSGMA
                     QRRLSIIDLSPGGHQPMRDDTGDLWIVFNGEIYNYKELGEEVRAQGVHLRSSSDTEIL
                     LYAYKIWGEDCLQKLNGMFAFAIYDVRKRKLFAARDRLGIKPFYYHFENGRLVFASEI
                     KAILNSTLVPKRPDLNALHTPARFQVSPLTGFEDILKLPPAHCLTLEDSQLSVRQYWG
                     LHPTEQEASEDVLIEQLDALLCDSVRLQMRADVPVGVFLSGGLDSSIISALMRVNTQQ
                     TIHSFTIKFSDEDQKFEQMTPDWDYARQVAKQFGFTYHEIEIHPQVEELLPKMVWHLD
                     EPLSDPAAINTYLISKAARDLDIVVLLNGMGGDEVFGGYRKHLACLTAESYQSIVPGM
                     VRNMVQYAADRIPVATSSRGLKQVRWGKRFLSIASLPRGDRFLMSDLSVPPNQFSQMF
                     ASRMSYYDSHFFKAQGPRFKSNGISYLTQMCLNDTLVFLPEHNLTYSDKASMAASIES
                     RPPLTDHRIVEFMFSVPPRERIHRTTQKYLLKKVAEKYLPENIIYRPKAPFASPLRSW
                     VRGPLSTMVADLLSEESLRARGLYDPAYVSGLIARDKQGLEDNAYQIWT"
     gene            complement(1889..3115)
                     /locus_tag="VEJ00_03075"
     CDS             complement(1889..3115)
                     /locus_tag="VEJ00_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXX00170.1"
                     /translation="MRTSRNILIIVQNLPVPFDRRVWMEATSLRRQGFGVAVVCPKKG
                     RCTASYERLEDVDIFRYPLVYEASKGVAGYFVEFVYCWLASLWLALKAYAHRPFHAIH
                     ACNPPDTFFALALLFRPLGVKFVFDHHDLCPEMYVAKGRPRTGLLYRGLVMLERLTLR
                     TADAVIAVNESHRMIARERGGISDDKISVVRSGPRRGWAEIHAPSPELKRGHANMVLY
                     LGEMCEQDGLVHLLHAIETYRAIAGDDTLFAFVGGGPDQPRMKAMAEEMGLGPVVHFT
                     GRIDDEQLWAYLSTADVCVDPDPLTEWSNMSTMNKIIEYMAFGRPIVAFDLAENRRSA
                     ESAAVYVQGNDDAALGRAIHNLLLNAEQREAMSQFGRARFREALAWENSEQRLIATYR
                     QLLDFPVPAFASASAD"
BASE COUNT          640 a          972 c          973 g          610 t
ORIGIN      
        1 tgtccagatc tggtaagcat tatcctccaa tccctgcttg tcgcgggcaa tcaatccgga
       61 aacgtaggcc ggatcgtaga ggccgcgggc ccgcagggat tcctcggaga gtagatcggc
      121 aaccatggtc gacagcggtc cccgcaccca ggagcgcaac ggcgaggcaa acggcgcctt
      181 ggggcggtag atgatgttct cgggcaggta cttttcggcc accttcttca gcagatactt
      241 ctgcgtcgtg cggtgaatgc gttcccgcgg aggaaccgag aacatgaact caacgatccg
      301 gtgatcggtg agcggcgggc ggctttcgat gctggctgcc atcgaggcct tgtcggaata
      361 cgtcaggttg tgctccggca gaaagaccag cgtgtcattc aggcacatct gcgtgagata
      421 ggagatcccg ttgctcttga agcgcgggcc ctgcgccttg aagaagtgcg agtcgtagta
      481 cgacatgcga ctcgcgaaca tctgggaaaa ctggttcggc ggaacggaaa ggtccgacat
      541 caggaaccgg tcgccccgcg gcagcgacgc tatggaaagg aagcgctttc cccagcgcac
      601 ctgcttcaag ccgcggcttg aagtcgccac cgggatgcga tccgcggcgt actgcaccat
      661 gttccgcacc atgccgggaa caatggactg ataactctcc gccgtcaggc aggcgagatg
      721 cttgcgatag ccgccaaaga cttcatcgcc gcccatgccg ttcagcagca ccacgatgtc
      781 gagatcgcgc gccgccttcg agatcagata ggtgttgatg gcggctgggt cggacagcgg
      841 ttcatcgagg tgccaaacca tcttgggcag cagctcctcg acctgcgggt ggatttcgat
      901 ctcgtgatag gtgaagccaa attgtttcgc gacctgccgg gcgtagtccc aatccggcgt
      961 catctgctcg aacttctggt cttcgtccga gaacttgatg gtgaaggaat ggattgtctg
     1021 ctgcgtgttg acgcgcatca gcgcgctgat gatggacgag tcgaggcctc cgctcaggaa
     1081 gacgccgacc ggcacatctg ccctcatctg cagccggacg gagtcgcaga gcagcgcgtc
     1141 gagctgctcg atgagcacat cttcgctggc ctcctgctcc gttgggtgca ggccccagta
     1201 ttggcgcacc gacagctggc tgtcttcgag ggtgaggcaa tgcgctggcg gaagtttcag
     1261 gatgtcctca aatccggtca agggcgacac ctggaagcgc gccggggtgt gcagtgcgtt
     1321 caagtcgggc cgcttcggga ccagcgtcga gttgaggatc gccttgattt ccgaggcgaa
     1381 caccaggcgc ccgttttcga agtggtagta gaacggtttg atgcccaggc ggtcgcgggc
     1441 agcgaaaagc ttccgcttcc gcacatcgta aatcgcgaag gcgaacatgc cgttcaattt
     1501 ctgcaggcag tcctcgcccc agatcttgta ggcatagagg aggatttccg tgtcgctgct
     1561 cgagcgcaag tgcacgccct gggcgcggac ttcctcgccc agttccttgt agttgtagat
     1621 ctctccgttg aagacgatcc acaagtcgcc ggtgtcgtcg cgcatgggct ggtggccgcc
     1681 cggcgagaga tcaataatgg acaatcgccg ctgcgccatg ccggagcagg cgtcgtcaaa
     1741 ccatttcaac ccggcatcgt ccggaccgcg gtgcgcaatg atgtcgttca tgcgccccag
     1801 cagttccgcg ctgccaccgt tcatcaatcc agctattccg cacatagtga gaagtcgtcg
     1861 ctatcgtgaa ttgagaatca aattggcctt agtctgcgct ggcgctcgcg aaggccggca
     1921 ccggaaaatc gagcaactgg cggtaggtcg caatcagccg ctgctcggaa ttttcccagg
     1981 ccagcgcttc ccgaaaacgc gcacgcccga actgagacat cgcctcccgc tgctcggcat
     2041 tgagcagcag gttgtggatc gcacgtccca gcgcggcgtc atcgttccct tggacgtaga
     2101 ctgcagccga ctccgcggag cgccggttct cggccagatc gaaggccacg atgggccggc
     2161 cgaacgccat gtactcgatg atcttgttca tcgtggacat gttgctccac tcggtgaggg
     2221 gatccggatc gacacagaca tcggcggtcg aaagatacgc ccacaactgc tcatcatcga
     2281 tgcggcctgt gaagtgtaca actggaccca ggcccatctc ctcagccatg gccttcatgc
     2341 gcggctggtc aggccctccg ccgacgaaag cgaaaagagt gtcatcgcct gcgatagcgc
     2401 ggtaggtctc gatcgcgtgc agcagatgaa ccaggccgtc ctgctcgcac atctcaccca
     2461 ggtacagcac catgttggcg tggccgcgct tgagttcagg agacggcgcg tgaatctcgg
     2521 cccatccccg gcgcgggccg cttcgcacca ccgagatttt gtcgtcggaa ataccgccac
     2581 gctcccgcgc gatcatgcgg tgagactcgt tgacggcaat gacggcatcc gccgtccgca
     2641 gggtgaggcg ttcaagcatc accaatcccc ggtagagcaa gcccgtacgc ggacggccct
     2701 tggcgacata catttccggg cagagatcgt gatggtcgaa gacaaacttc actcccagtg
     2761 gccggaacag caacgccagc gcgaaaaaag tgtcgggtgg attgcaggca tgaatggcat
     2821 ggaacgggcg atgggcgtac gccttcagcg ccagccacaa cgaggccagc cagcaataga
     2881 cgaattccac gaagtatccc gccacgcctt tactcgcttc gtacaccagc ggatagcgaa
     2941 aaatatccac atcttccaat cgctcgtagc tggcggtgca gcgacctttc ttggggcaga
     3001 cgacggcaac gccaaacccc tggcggcgca ggctcgtcgc ctccatccac acgcgccggt
     3061 caaacgggac cggcaggttc tgcacgataa tcaggatgtt gcggctcgtt ctcatatttc
     3121 aagtaccagt tcctgttcca ccactcccgc cgacgttgcc gcgcggcgcg ctgtgaggtc
     3181 gatgacggac gcccg
//
LOCUS       DASPUL010000122        14247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1663927, whole genome shotgun sequence.
ACCESSION   DASPUL010000122 DASPUL010000000
VERSION     DASPUL010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 14247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14247)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14247
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1663927"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2576
                     /locus_tag="VEJ00_03080"
     CDS             <1..2576
                     /locus_tag="VEJ00_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXX00171.1"
                     /translation="IVKDKTFYFVDYEGSRLKDGAPTLFTSETPSFRQLVTTNNPGSL
                     AAMFYHDFPGPACTGPAFGVDGQYCSAVSSQLERNQADQYLVRVDQHLGTHDQLYGRW
                     VNTLASGDVARQELEGAGTRGFEAPFDGFFADLSIGETHEFTARTMNDLRVAYSRNNS
                     SIDFALPPNTLTGAALKAAGAAPDAFGQLVFDDGVVPMGGEVFVPRKFIFNTYAFTDT
                     LSHIVGRHALKFGFQLRRVQENSDYQLTSKPFFEFSCIDSDTCFQSFGGATPSTAANP
                     AGDLPYLEAALVNRMNCTGSPSPTCGQFTDTPRHFRWSQYAAFFQDDWKVTPRLTMNL
                     GLRWEMFGSPTEANGILSNIILGPGSNLFQQIAGATVGRVSKMWNTDKKDFAPRVGMS
                     WDPTGKGETVVRSGFSIAYNEPYSNLYTNASRLDPPDAITTFVDPGAFIGTPGSVVYG
                     PGAFPFVPSPNFAGPTLPNGGIGPAINGVDITPSGVDPHLRMAYSMQWFLGIQHQFLH
                     DYGISVNYVGTRGVGGYTREDDNRFDGDICQTLANGFGPTCTLFNQRLNLGWGQLTYV
                     ANESQSIYHGLNAQLRRTYTHGLMFAANYTYGKVLDNVTEGGLGDYFNTNGYGGLYSG
                     VQDIENPDGDRGPSEFDARHRFAFSALWAIPSPTSNAALKNVLGGWKLNSIISLQSGR
                     PFDVYCGLAWFSGCDFNMDGLAYDRPNRPAGIKTAGFSNHEFESGLFGDPTLTFSATG
                     DSRTSPASQVFCPNGLNSILDTLFLNAALTPCLPVGTNGNLSRNAFRGPAYKTVDLAL
                     LKDVKAGDRFTVEFQAQAFNLFNRVNLYNPIGDLGSLNFGRSISAFPARQIQLGLKLS
                     F"
     gene            2781..4523
                     /locus_tag="VEJ00_03085"
     CDS             2781..4523
                     /locus_tag="VEJ00_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00172.1"
                     /translation="MLTRGTLMGASTPPAPVDDSAGFVIPAEAAEPKDHFEGTLTLNN
                     TGENGKFTLLSEIFGLVSTDGDSPWKHLPPMHIQFVQSGSQLIPVQQGLVISGSPVWN
                     YIIGPGRVWQERGDEGYTRAAFPFALIQRNQNCVHNGEMTFLFKNTKNPSISSVYYQI
                     TQETCYPMKFDMWGIVPATYSPVSIANAQAIANSQAREFSQRMPRKPFSDLEKDFNNS
                     GIDLSAFAAAYKRKQDMTTYGAVVGGVNYSTGCPTRYGEYAFCEEMRLPSYSIAKSAF
                     AGVALMRLGQLYGRSIYAELIRKYLPEYKDGGNWDTTTFNNVSDMATGNYNLPGYEAD
                     EDSSAMDVFLVAEDQEAKLKAAFAINKKFATPGTKWVYQSSATFVLTQAMNTYLQQKQ
                     GNGADLFNMVRDDLYIPLHVSQGGLTTIRTSNSPEGAPSGYYGLFFIKDDVARIGTFL
                     NNSYGVLDGVQMLEPSRLKEALFRNADPASSGVSIEDSNPASLLGKPDYGSGPAVPSS
                     RRYSHGFWGRYISKQEFPQFPCNFWSAFMSGYGGNIVALLPNGVTFYSFTDGNEFPWT
                     SAVSELAKLGPLCK"
     gene            complement(4626..5213)
                     /locus_tag="VEJ00_03090"
     CDS             complement(4626..5213)
                     /locus_tag="VEJ00_03090"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HXX00173.1"
                     /translation="MKALMCSALVVLTCLVLLSSRPEGKGPVPQYNQKGELLRPADYR
                     EWMFLSAGYGMNYSPGPDSHEMFTNVFVQRWAYDEFARSGKWPEQSMFVIDERDAASR
                     SSINQKGHYQTDLMGLAVEVKDSARNSDKWAYYVFTPDGQTAGAMPKGNPCWSCHDAH
                     AAVEHTFVQFYPTLKVVAKKYGTYNESREQVTDAK"
     gene            complement(5876..6133)
                     /locus_tag="VEJ00_03095"
     CDS             complement(5876..6133)
                     /locus_tag="VEJ00_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXX00174.1"
                     /translation="MTCSRYSVGLLLVVAFVLGFALTQPAYADNLYASIRGTVVDPTG
                     AVVPDAKLTATNVATGLSYSTTWRDSGQNRSKIADTKRQIA"
     gene            6717..9092
                     /locus_tag="VEJ00_03100"
     CDS             6717..9092
                     /locus_tag="VEJ00_03100"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXX00175.1"
                     /translation="MVFTRGKTRPLQRSSTKEGWWWQRIAPFVLLACATGVQAQHARG
                     ELRIEVHDPHGGAPASEAELVSNGNQFRRSFQITHGRYVVQELPFGIYRLTVKAEGFA
                     PWTDVLEVRSEVPVQIAVTLGLAPVTTQIEVTDSVTLVDPNGTGTQYSIGHQVLAENI
                     PAQPGRDLSDLVDELPGWLYEANGVLHPRGSEYDVQYVVNGVPVSENRSPAFAPSLDA
                     DDVESMRVLTADYPAEYGRKLGGVIEVTTEKDVPSGLHGRINLDGGSFSTAGGAGALS
                     YVLGGNRFALSGDGFHTERYLDPPVLENFTNTANASGVSGSYEREFSERDRMRITVTH
                     DVTRFLVPNYLVQQAAGQRQNAADAETSGQISLEHLISPDLLLRLSGSVRDAAADLSS
                     NSQSTPVIASQDRGYREGYVRGDLAGQHGQQNWKAGLDGIFDPVHEELQYRITDPTQF
                     DAGTQSQFQFSDRRWDVEPSAYVQDQIHLKNWNLSAGLRFDHYGFVVRESAWSPRVGV
                     SRYVQSPNLLIHASYDRVFQTPAIENLLLASSPQVDSLNPVVVRLPVRPGRANYYEVG
                     VSEALLGRLRLDANVFRRDFRNYSDDDVLLDTGISFPIAFARARILGEELRLEVPHWG
                     RFSGYLSYANQSGTGQGPITGGLFLGSDAANQLTDTSKFAVSQDQRNTARIRLRFQAA
                     HRMWLALGGEYGSGLPADTGSTDPSLLLAQYGAAVLDRVNLARERVRPNLSADAAAGT
                     EFYRKEHRTASFQIQAANLNNRVNVINFASLFSGTAVAPPRSVSARLQLTF"
     gene            complement(9292..11370)
                     /locus_tag="VEJ00_03105"
     CDS             complement(9292..11370)
                     /locus_tag="VEJ00_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXX00176.1"
                     /translation="MLAVMAELALLCSALPAQQEDYTFRVQTDLVLVNVTVRDKNGNI
                     VTNLKPDDFTILEDGKPQKVVSFDIENVDAVANQDVAQARPLAGPPSTSLPAVPAAPA
                     KTAEEFKDRRLIVLFFDLSAMEPDEIDRAVTSAEHYVDTQMAPADLVSIVSLGSALLV
                     NQDFTSDHDLLKKQLQAFGVGSGQGYEEGTTGTTEGTPDTGQPFTADDTEYNIFNTDR
                     RLEALRSVAEKLAHLQQKKSLIYFSSGMDRTGIENQSELRAAINAAVRSNLAIYTMDL
                     RGLQALVAGGEAQNASLRGTSAYSGQSMINALSSNFTTQETLVTLASDTGGRAFLDSN
                     DFSQVFKGVQQDTASYYLLGYRSTNPARDGRYRRIVVKVNVPGVKLDYRRGYYAPADY
                     QHSTKEDKELQLQQELASDLPTTDLPLYLSTAYFRLAANIFFVPISLVVPGSQIPFTR
                     ASDRDKATLDVIGLVLDDQHHPVTQIRDTVKLAVDSSSEVRKKNVQYDTGVSLPPGKY
                     HLKFVVRENQTGRMGSFETDLTVPDLKSQPLKISSIVVASQLQTAKKGTALNPLVRDG
                     EEIVPNVTHVFSAGQHLLLYYEVYDPGRSNTAQASQTGAANSAIRLLTSVAFFRGKAK
                     VFESSQVELTQLNARDRKAGVFQLDLPLSSLSPGFYTAQINVIDDAAGSFLFPRFALL
                     IRPEGKIGAH"
     gene            complement(11418..>14247)
                     /locus_tag="VEJ00_03110"
     CDS             complement(11418..>14247)
                     /locus_tag="VEJ00_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00177.1"
                     /translation="GGFGGGGPGRPMTITGRRGFDINHPHGSIYYGVGDGALNAAPYS
                     LTGQPVVKPAYLQNSFGGSVGGPLNIPRIYHGGTKTFFFANYNGRHGENPFDQFSTVP
                     TLAERQGNFNSVLYTSGPAAGQQVQIFNPATNAPFSGNVIPGFASQTCPWSAATGGGI
                     PGGLLQYIPCPNLPGNFQNFHFVTSTTNNSDDLNVRINHTIGAAAPARGRRGGGRGGP
                     RNDVNFGFHYHGSSANITNPFPSVGGSTSVRSFDVPLGYVRTFGKLTNNLRFDFNRSR
                     THTQNLYAFLNDVDAGLGITGVSTNPFDWGLPNLSFADLSGLQDTNPQLIRNQTYTIS
                     DYVVYTHGKNSWRWGGDFRRIELNTETSGNARGSFTFTGYNTSELAATAPGQPAAQVP
                     GTGFDFADFLLGLPQTTSVQFGQDNYHFHGNYWDLYAQDEWKVHANFTLNLGVRYEYV
                     SPLTEENNLIANLDLSPGVLNPFAGAASVAVVTPGQAGPFSGSLPASLVRPDHKGFAP
                     RTGFAWKPFSKTVVRGGYGINYNTGAYQSIALQMAFQPPFSTAETNIQPIVVTPSTGG
                     LVPTAGALTLGSGFPTPAAGEITNSYAVNPNYRWGYVQIRNLDIQQQIRPTLLLNIDY
                     TGTKGTNLDILEAPNRTISPTLGITTLLQGAQAFTYENSLGDSEANAASVRLRKRLSS
                     GFSVGGLYTFSKSIDDASSIGAGATSAAGSPGLGAGGTGAVGRGAGASAGSGANSVAQ
                     DPLNLSAERGLSSFNQTHRFTADYLWELPLGHDKRWLTGNTPWRAILGDWQWSGDWTI
                     ASGLPFTPRFVNNADEINGGTNGTLRPDIVPGQPISLPHPSIAEWFNKAAFAAPPENA
                     QGQVIAYGNARRNSIIGPGSKVFDMALTKVIPLNEGRTLEFRAQATNAFNIPNYSSID
                     TSVTSPTFGRVTAVGSMRQITMTARFRF"
BASE COUNT         2915 a         4060 c         4214 g         3058 t
ORIGIN      
        1 cgatcgtcaa agacaagact ttttactttg tggattacga aggctctcgg ctcaaggacg
       61 gcgcgccaac gctgtttacg agcgaaacac cctcgtttcg gcagttggtg accaccaaca
      121 atccgggaag cttggcggct atgttctacc acgactttcc cggccccgcc tgcactggtc
      181 cggcttttgg tgtagacggc cagtattgct ccgcggtttc gtctcagctc gagcgcaatc
      241 aggctgacca ataccttgtc cgtgtcgatc agcatctggg aacgcacgac cagctctatg
      301 gacgctgggt gaatacactg gcgtcaggcg atgtggcacg ccaggagctg gagggcgcag
      361 gtacgcgagg cttcgaggcg ccttttgacg gttttttcgc cgaccttagc atcggagaaa
      421 ctcacgaatt caccgcccgc acgatgaatg accttcgcgt ggcctactcc agaaacaaca
      481 gcagcatcga cttcgcgctt ccccccaaca ccttgactgg cgctgcgctg aaagcggctg
      541 gcgcggcgcc cgatgcgttc ggtcaactgg tctttgacga cggcgttgtt cccatgggtg
      601 gcgaggtttt cgtcccgcgg aaattcatct ttaacaccta tgcgttcacg gataccctta
      661 gtcacattgt gggccggcac gctctgaagt ttggctttca gcttcgccgg gtccaggaaa
      721 acagtgacta ccagctgacc tcgaagccct tcttcgaatt ctcctgcatc gacagcgaca
      781 cctgcttcca gagcttcggc ggcgcgactc cgtccaccgc tgcaaacccg gctggagatt
      841 tgccctatct ggaggctgcc ctcgtcaacc gcatgaattg cacgggctca ccgtcgccca
      901 cctgcggtca atttacagat acgccccgcc atttccgctg gagccaatac gcggcgttct
      961 tccaggacga ttggaaggta actccccggc tcaccatgaa cctgggattg cgctgggaga
     1021 tgttcggatc gcccaccgaa gccaacggga ttctctccaa tatcatcctt ggtcccggca
     1081 gcaatttgtt tcagcagatc gcaggcgcca ccgtcggccg ggtcagtaag atgtggaata
     1141 ccgacaagaa ggatttcgcg ccacgagtgg gaatgtcctg ggatccaacc ggcaaaggcg
     1201 agacggtcgt gcgcagcggc ttcagcattg cctacaacga accttattca aatctctaca
     1261 ccaacgcctc ccggcttgat cctcccgatg ccatcaccac ctttgtggat cctggagcct
     1321 ttattggaac tccgggctcg gtggtctacg ggcccggcgc atttcccttc gtaccgagtc
     1381 cgaactttgc gggtcccact ctccccaatg gaggcattgg acctgctatc aacggcgttg
     1441 acataactcc cagcggagtg gatccacatc tgcgcatggc ctattcgatg cagtggttcc
     1501 tggggattca gcaccagttt ctccacgact acggcatcag tgtgaattac gtcggcacgc
     1561 ggggagtcgg cggttacaca cgggaagacg acaaccgctt cgatggagat atctgtcaaa
     1621 ccctggccaa cggtttcggt ccaacctgca ctctcttcaa tcagcggctg aacctgggct
     1681 gggggcaact cacctacgtc gccaatgaaa gccaatccat ttaccacggc ttgaatgctc
     1741 aactgcgcag gacttacacc cacgggctta tgttcgccgc gaactatacg tacgggaagg
     1801 tgctcgacaa tgtgaccgag ggtggattag gcgactactt caacaccaac gggtatggcg
     1861 ggctctatag cggcgtgcag gacatcgaga atcctgacgg cgaccgtggt ccctccgagt
     1921 ttgatgcccg gcatcgtttc gcattctcgg cgttatgggc tataccttct ccgacttcca
     1981 acgccgcttt gaagaatgtt ctgggaggct ggaaattgaa ttccatcatc agcctgcaat
     2041 cggggcgtcc gtttgatgtg tactgtgggc tggcgtggtt cagcggttgc gacttcaaca
     2101 tggacgggct ggcgtacgat cgcccgaatc gcccggcagg aattaagacc gccgggttta
     2161 gcaatcacga gtttgagagc ggactttttg gagacccgac cctgacattt tctgcgaccg
     2221 gcgacagccg cacctctccg gcgagtcagg tgttctgtcc caatggtctc aactccatcc
     2281 tcgatactct gttcttgaac gcagctctga ctccgtgtct gcccgtcggc accaacggaa
     2341 atctgagccg gaacgcattc cgcggtcccg cctacaagac ggtcgacctg gcgctgttga
     2401 aggatgtgaa agccggcgat cgattcacgg tggagttcca ggcccaggct ttcaacctgt
     2461 ttaaccgggt aaatctgtac aacccgattg gcgacctcgg cagcctgaac tttggccgct
     2521 ctatatcggc gttcccggcg cggcagattc agttgggatt gaaactgtct ttctagttcc
     2581 tcgccaacgc agtggtacga atcgtgccgc aattgacatc aactcgccta aagacgaact
     2641 ctgtcccgtg cagagcactg aactgctgcg cggcattcct ggttgcgacc gcgttggtcg
     2701 gcggggcgcg cgcgcaaact actgagagca gatctccgtt cggcccaccg ctttcggggt
     2761 cgggcaaggt cgatcgagcc gtgctgactc gcggcacgct gatgggtgca agtacgccgc
     2821 cggcgcccgt cgatgacagt gccggtttcg tcattcccgc agaggctgcc gagcctaagg
     2881 atcactttga gggcacgctg accctgaaca acacgggaga gaacgggaag tttaccttac
     2941 tgtctgagat tttcgggttg gtgtcgaccg atggcgattc tccgtggaag caccttccac
     3001 cgatgcacat ccagttcgtg cagagcggca gtcagttgat tccggtgcag caggggctcg
     3061 tgatttccgg cagtccggtc tggaactaca tcatcgggcc gggacgcgtg tggcaggagc
     3121 gcggagacga aggttacacg cgtgcagcat ttccttttgc cctgattcag cgcaatcaga
     3181 attgtgtcca caacggcgag atgacgtttc tcttcaaaaa cacaaaaaac cccagcattt
     3241 cgagcgtcta ctatcagatc acgcaggaaa cttgttaccc aatgaagttt gacatgtggg
     3301 gcattgtgcc ggcgacttac tcgccggtct cgatcgcgaa cgctcaagcc attgcaaaca
     3361 gtcaagcgcg cgagttctca cagcggatgc cccggaagcc gttcagcgat ttggaaaagg
     3421 acttcaacaa ctccggtatt gatctgagcg ctttcgcagc cgcttacaag cgcaagcagg
     3481 atatgacgac gtatggagca gtcgttggcg gcgtgaatta ttcgacaggt tgcccgacgc
     3541 gctatggcga atatgccttc tgcgaggaaa tgaggctgcc ttcatattcc atcgccaagt
     3601 ctgcctttgc tggtgtggca ctgatgcggc ttggacagct ctatggtcgc agcatatatg
     3661 cggaactgat caggaagtac ttgccggaat acaaggatgg cgggaactgg gataccacca
     3721 cgttcaacaa cgtttctgac atggctacgg gcaactacaa cctgcccgga tacgaagccg
     3781 atgaagacag ttcagccatg gacgtatttc ttgtggccga ggaccaggaa gcgaaattga
     3841 aggccgcttt tgccatcaac aagaagttcg ctactcccgg cacgaagtgg gtctaccaaa
     3901 gttcggccac gtttgttctg acgcaagcga tgaacacgta tctgcagcag aaacaaggaa
     3961 acggcgcgga cctgttcaac atggtgcgtg atgacctgta catccctttg catgtgagtc
     4021 agggcgggtt gacgactatt agaacgagca acagcccgga gggcgcgccc agcggatact
     4081 atggcttgtt cttcatcaag gacgacgttg caagaatcgg taccttcttg aacaacagct
     4141 atggcgtgct ggacggcgtt caaatgctgg agccgtcgcg gctgaaggag gcgttatttc
     4201 gcaacgcgga cccggcgtcg tccggagttt ccatcgaaga cagcaatccc gcatcattgc
     4261 tgggcaaacc cgattatggc agcggccccg ccgtgcccag cagtcggcgg tattcccatg
     4321 gattctgggg tcgctacatc agcaagcagg agtttccgca gtttccatgt aatttttgga
     4381 gtgcattcat gagcggttac ggcggcaaca ttgtggcgct gcttccgaat ggcgtcacgt
     4441 tctacagttt caccgacggc aacgagttcc cgtggaccag cgcggtttcc gagttggcga
     4501 agctcgggcc actttgtaaa tgaaaactgc tcggggcgcc ggacgcttgg cttcgtcctc
     4561 ctccgatagt agtacagttt gaatctgggc gaagcgccag gcaaagtcca gtgtgtcaag
     4621 ggcccttact tcgcatccgt cacctgctcg cgcgactcat tgtaagtacc gtatttcttc
     4681 gcgacgactt tgagtgtggg atagaactgc acgaatgtgt gctcgaccgc ggcgtgggca
     4741 tcgtggcagg accagcacgg attgcctttg ggcatggcac cagcggtttg gccgtccggg
     4801 gtgaaaacgt aatacgccca cttgtcggag ttgcgcgccg aatctttcac ctcaacggcg
     4861 agccccatca agtcggtctg atagtggcct ttctggttga tggaactgcg gctggcggcg
     4921 tcgcgctcgt cgatcacgaa catgctctgc tccggccact tgccggatct cgcaaactcg
     4981 tcatacgccc agcgctgcac gaagacgttg gtgaacattt cgtgactgtc agggccggga
     5041 ctatagttca tgccgtagcc ggcggaaagg aacatccatt cgcgatagtc ggcagggcgc
     5101 aacagttcgc ctttctggtt atattgcggc acaggccctt ttccctcggg gcgcgaggaa
     5161 agcagcacca ggcaagtcag gacaacgagt gcagaacaca taagagcttt catagtaagt
     5221 tctccatcgg gggaatccat gagcgtcgcc ggatagttcc tgcttggcag caaagccctc
     5281 catgccgatc cacaacggcg ccagaagttg cagattcctc ctgcaagcac gaggggccgt
     5341 gaacgctctt gctccccgca cgaagcgggt tgtggtccgt gcatgaatct tctcaaccaa
     5401 agctgcgttc ttcctctact acaaaacctg gcagtcggga tccttctctt gatgtcccct
     5461 ccaggtccat ttccgtatgc caccgcgttc cacgggaaca gccagcgcaa ggttggcaag
     5521 ttgatgaagg ctcttgctga ccaggcctaa cgggtcgttg caaagcacaa tcaggtttgc
     5581 gttcgattgt agttcggcgc aaacgcttcg ttcgatctgg tttcgacgag cttacaagat
     5641 cagcgcctat tgcactatca actccggaaa cgagcttttc aatgatttgc aacttttcgg
     5701 ttgtgtataa gcggaattcg gcaagcaagg agagctcttt ggagagagaa aaccctgaga
     5761 ggcttccgga atgcttcatg tgatcagttc agttcttcga cagttcactc accaccggta
     5821 gaaatgcttc gtatcgctgc cggcgtgcac tttgtgcgtc cgccagcttc cccgatcaag
     5881 caatctgccg cttcgtatct gcgatcttgc tgcgattctg gccggaatct ctccaggtcg
     5941 tggagtagct gaggccagta gccacgttcg tggctgtcaa tttcgcgtcc ggcacaactg
     6001 cgccagtagg atcgaccacc gtcccgcgaa tactggcgta aaggttgtca gcataggccg
     6061 gctgcgtcag agcgaatccc aacacaaacg ccacgaccaa cagtaatccc accgagtaac
     6121 gactgcacgt catagaggct gcctcctggg caaacacgct gatagctgtc atcaacgtct
     6181 gtgcaggcgg agggccgaag gtctcccggc cgtaaccttt tcatgagcaa ttacctacgg
     6241 aaccggccag cagcggtcct ttccatttca cagaaaggcg ggacgaatca tgtcgtagga
     6301 attgttgttc ttgaattgca ccgacgtatg gtcagcgcga tcttgtgttt ggttacagca
     6361 tcgcggagaa gtagggcgca aaatcccaaa tcgtgaatgt gcgtgccagc aatccggcga
     6421 tggcgacggc ttcacggaat ccgaacgact atatgaagct agaaactatc tgagaaatgg
     6481 cactgggagg aacgccaccg cagagtttcg ctggacgggc tgtggcgaat gtgcccggta
     6541 gcacctgccc ccttgcagcc gggttcaagg ctcgcgaaaa tgtccgtcgt gaacgagggc
     6601 ggcaggtgat aggttccaac ttatgataag gaacgactcg ggatcgtcac cttgtagggt
     6661 aaacgcggag gagtgggata ttctgtaatt cttggggtgc gtcacgctca gagcgaatgg
     6721 tcttcacacg gggaaagaca cggccactgc agcggtcgtc gacaaaagag gggtggtggt
     6781 ggcagcggat tgctccgttc gtactcctgg cctgcgctac gggtgtgcaa gcgcagcacg
     6841 cgagagggga gctgcgcatc gaggtgcatg atccccacgg tggagcaccg gcctccgagg
     6901 cggagttggt gagcaacggg aatcaattcc gccgcagttt ccagatcact catggccgtt
     6961 atgttgtaca ggagcttccc tttggcatct accggctgac tgtgaaggcc gagggatttg
     7021 ccccgtggac cgatgtgctc gaagttcgat cggaggtgcc ggtacagatc gcagtgactc
     7081 tgggcctggc acccgttacc acgcagattg aagtcacaga ctcagtgaca ctggtcgacc
     7141 ccaatggcac aggcactcag tactcgatcg gacaccaggt tctggccgag aacattcctg
     7201 cacaaccggg aagggacctt tccgacctcg tcgacgaact gccgggatgg ttgtacgagg
     7261 ccaacggtgt gctgcatcca cgaggctcag agtacgacgt gcaatacgtc gtgaacggag
     7321 tgccggtcag cgagaaccgg tcgccagcct ttgcaccatc gctggatgcc gatgacgttg
     7381 aatcgatgcg ggtattgacc gcagactatc ccgccgagta tggcagaaaa ctcgggggcg
     7441 tgattgaagt aacgaccgag aaagacgttc cttccgggct ccacggaagg atcaacctgg
     7501 acggagggag cttttcgacg gcggggggcg caggcgcgct ttcctatgtg cttgggggga
     7561 accggttcgc gctcagcggt gatggcttcc atactgagcg ctatttggac ccgcccgtcc
     7621 tcgagaactt cacgaatacc gcaaatgcga gtggcgtttc cgggtcttat gagcgggagt
     7681 tctcggagcg cgaccggatg cgcatcacgg tcacgcacga cgtaacgcga tttcttgttc
     7741 cgaactacct cgtccagcaa gccgccggac agcgccagaa cgccgccgat gccgagacga
     7801 gcggtcagat ttcactcgag catctcattt ctcctgatct attgcttagg ctctccggca
     7861 gcgtgcggga tgcagcagcg gatttgtctt ccaattctca gtcgactccc gtcatcgctt
     7921 cgcaggaccg cggctatcgc gaaggttacg tgcgcggaga cctggccggg cagcatggcc
     7981 agcagaactg gaaagcaggc ctggacggaa tctttgaccc ggtccatgaa gaactgcagt
     8041 acaggatcac cgatccgacc cagtttgatg cgggcaccca gtctcaattt caattctccg
     8101 accggcggtg ggatgtggag ccttctgcct atgtgcagga ccagatacat ctgaagaact
     8161 ggaacctgtc ggcaggcttg cgcttcgacc actatggctt tgtcgtgcgc gagtcggcgt
     8221 ggagtccgag agtcggcgtt tcgcgctatg tgcagtcgcc gaatctattg atccatgcgt
     8281 cctatgaccg tgtgtttcag actccggcga tcgagaacct gctgctggcg agctcgcctc
     8341 aagtcgattc cttgaatcct gtcgtcgtgc gactgcccgt tcggccaggc cgtgccaact
     8401 actatgaggt cggcgtcagc gaggccctat tgggacgact gcgtctcgac gcgaacgtgt
     8461 ttcggcgtga ttttcgcaac tattccgacg atgatgtgct gctcgacacc ggcatcagtt
     8521 tcccgatcgc ctttgcccgc gctcggatcc tcggtgaaga gttgcggctg gaagtgcctc
     8581 actggggaag attctcgggt tacctgagct acgcgaatca gtccggcacc ggccagggcc
     8641 cgatcacggg cggtctgttt ctgggcagcg acgcggccaa ccagttgacg gatacgagca
     8701 agtttgcggt gtcgcaggac caacggaaca cggcgcgcat ccggctccgc ttccaggcgg
     8761 cgcatcgaat gtggctagct ctgggcggcg agtatggaag cggactgccc gccgacacag
     8821 gcagcacaga cccgagtctt ttgctggccc agtacggagc cgcggttctt gatcgggtga
     8881 acctggcgcg cgaacgtgtg cgtccgaatc tttccgcgga tgctgctgcc ggaacggaat
     8941 tctaccggaa ggaacaccgc actgcttcgt ttcaaatcca ggccgcgaat ctgaacaacc
     9001 gcgtgaatgt catcaatttc gccagcctgt tttccggaac ggcggttgcg cccccccgaa
     9061 gcgtttcggc gcgcctgcaa ttaacgttct agggcactac ctcaaccttg cctatttcga
     9121 ggcctcctag ctccggaaca ccacgtcagc agtgattggc ttcgtccttc tccgatagcg
     9181 gtacagtttg agtctgggcg aagtgccggg caaagtctca gcgtgtttgg gcaacagggt
     9241 caggctacaa cctgtcccat ctcattagtg cattcgggac gcggcatatc cctagtgggc
     9301 cccaatcttg ccttcgggac gaatcagaag agcaaacctt gggaagagga aagaaccggc
     9361 agcgtcgtcg atgacattga tctgggcagt gtagaatccg gggctaagcg agctaagcgg
     9421 caggtcgagc tggaagacgc ctgccttccg gtcgcgggca tttaattgcg tgagctcaac
     9481 ctgggagctc tcgaacactt tcgctttccc ccggaaaaag gcaacgctgg tcagcaggcg
     9541 gatcgcagaa ttggctgctc cggtttgcga ggcttgcgcc gtatttgacc gccccgggtc
     9601 gtacacttcg tagtagagga gcaggtgctg cccggcagaa aatacgtggg tgacgttggg
     9661 aacaatctct tcgccgtcgc gaaccagcgg gttgagagct gtaccctttt tggcggtctg
     9721 cagctggctg gcaacgacaa ttgaactgat cttgagcggc tgcgatttga ggtcggggac
     9781 ggtcaggtcg gtctcgaacg accccatgcg gccggtctgg ttctctcgca ccacaaactt
     9841 gaggtgatac ttgcccggag gcagactgac accagtgtcg tactgaacgt ttttcttgcg
     9901 gacctcggag gaactatcga ccgcgagctt caccgtgtcg cgaatctggg tgacggggtg
     9961 gtgttggtcg tcgagcacca gaccaatgac gtcgagagtc gctttgtcac ggtcgctggc
    10021 gcgcgtaaag ggaatctgag agcccggcac gaccaaggaa atcggaacaa aaaatatgtt
    10081 ggccgccagt cgaaagtagg ctgtgctcag atacaggggc agatctgtag tgggcaagtc
    10141 ggaagccagt tcctgttgaa gctggagctc cttgtcctcc ttcgtggagt gctgataatc
    10201 ggccggagcg tagtagccgc ggcggtagtc cagcttgaca ccaggcacgt tcaccttgac
    10261 gacgatgcgg cggtagcgcc cgtccctggc cgggttggtg ctgcgataac cgagcagata
    10321 gtagctcgct gtgtcctgct gcactccttt gaaaacctgg ctgaagtcgt tgctgtcgag
    10381 aaaggcgcgc ccgccggtat cgctggccag cgtaaccagc gtttcctgcg tggtgaagtt
    10441 agaactcaga gcgttgatca tcgactggcc ggaataagcg gaggttcccc gcaggcttgc
    10501 gttctgcgcc tctccgccgg cgaccagcgc ctgcaggccg cgcaggtcca tggtgtagat
    10561 agccaggttc gagcgcacgg cggcattgat ggcagctcgc aattccgact ggttttcgat
    10621 gccggtcctg tccatcccgc tggaaaagta aattaaagac ttcttctgct ggagatgcgc
    10681 cagcttctcc gcgactgagc gaagcgcttc cagccgtcga tcggtgttga agatgttgta
    10741 ttccgtatcg tctgcggtga atggctgtcc ggtgtcggga gtgccctctg tcgtgcctgt
    10801 cgtgccttct tcgtagccct gaccgctccc cactccgaaa gcctggagct gcttcttgag
    10861 caagtcgtgg tcggaagtga agtcctgatt caccagcagc gcgctcccca gcgacacaat
    10921 cgagaccagg tccgcaggag ccatctgggt gtcgacgtag tgctcggcag aagtgacggc
    10981 tcgatcgatt tcgtcaggtt ccatggcgga aagatcaaaa aacagaacga tgagccgtcg
    11041 atccttgaat tcttccgctg tcttcgcggg ggcggcaggg accgctggca ggctcgtgga
    11101 tggaggcccg gccaaaggcc gtgcctgagc aacatcctgg tttgccacag catcgacgtt
    11161 ttcgatatcg aaggaaacaa ccttctgagg cttgccgtct tcgagaatcg tgaagtcgtc
    11221 cggcttcaag tttgtaacga tatttccgtt cttgtctctg accgtgacgt tgaccagcac
    11281 aagatctgtc tgcacacgaa aggtatagtc ttcctgctgc gccggcagag ccgaacagag
    11341 aagcgcgagt tccgccatca ccgccagcat ctttctggct attcgtcgat ttggatggca
    11401 ggacacgttt gactctctca aaaacggaag cgggcagtca tcgtaatttg ccgcatagac
    11461 ccgaccgctg taacccgtcc aaaagtgggc gacgtcaccg acgtatcgat cgagctatag
    11521 ttcggaatgt tgaaggcgtt ggttgcctgt gcccggaact ccagcgtccg cccttcatta
    11581 agcgggatga ccttggtcag cgccatgtcg aacaccttgc tccccggccc gatgatgcta
    11641 ttgcggcggg cattgccata cgctatcacc tggccctgag cattctcggg aggcgctgca
    11701 aatgccgcct tattgaacca ctccgcgatc gagggatgcg gcagcgaaat cggctgtccg
    11761 ggcactatat ccgggcgcag cgtcccattc gttcctccgt tgatctcatc ggcattgttt
    11821 acgaagcggg gagtgaacgg aaggccggaa gcaatggtcc agtcgccgct ccattgccaa
    11881 tcgccaagaa ttgcgcgcca cggcgtgttg ccagtaagcc atcgcttgtc atgccccaag
    11941 ggcagttccc aaagataatc cgcggtgaat cgatgcgtct gattgaagct ggagagccct
    12001 cgttccgcgg agaggttcaa cgggtcctgg gccacgctgt tggccccact gccggctgat
    12061 gcgcccgcgc ctcttcctac agcgcctgtg cctccggcgc ccaagccggg cgagccggca
    12121 gccgaggttg ctccagctcc aatcgaggat gcatcatcga tcgacttcga gaacgtatac
    12181 agcccgccga cggagaaccc gctcgacagt cgcttgcgga gccgcaccga agcggcgttc
    12241 gcctcggaat cgcccagcga gttctcgtaa gtgaatgcct gcgcgccctg cagtaaagtc
    12301 gtaatgccca acgtgggcga gatggtgcgg ttcggcgcct ccagaatatc gagattcgtt
    12361 cccttcgttc ccgtgtaatc gatgttcagg agcaacgtcg ggcgaatctg ctgctgaatg
    12421 tcgagattgc ggatctgcac atatccccac cgatagttcg ggttcaccgc gtaactgttc
    12481 gtgatctcgc cggcggcagg cgtggggaag ccgctgccga gcgtcagggc tccggccgtg
    12541 ggtacgagcc ctcctgtcga gggcgtgacc acgatcggct ggatgttggt ctcggcggtg
    12601 gaaaacggag gctgaaacgc catttgcagc gcgatcgact gataggctcc ggtgttgtag
    12661 ttgatgccat agcctccgcg gaccactgtc tttgaaaatg gcttccacgc gaatcccgtg
    12721 cgtggggcaa accctttgtg gtctggccgc actaggctgg cgggcaggct gccgctgaag
    12781 ggtccggcct ggcccggcgt gactaccgcg acagacgccg cgcccgcaaa cggattcagc
    12841 acccccggag acagatcgag gttcgcgatg agattgtttt cttccgtcag cggcgacaca
    12901 tactcatagc gcacgcccag attgagcgtg aaattggcat gcaccttcca ttcgtcttgg
    12961 gcatacaaat cccagtagtt cccgtgaaag tggtagttgt cctgcccgaa ttggacggag
    13021 gtcgtctggg gcaacccgag gagaaagtcg gcgaagtcaa acccggttcc cgggacctgc
    13081 gcggccggtt gccccggcgc cgtcgcagcc aattccgagg tgttgtatcc ggtgaaggtg
    13141 aacgagcccc gcgcgttgcc actggtctcg gtattgagct caatgcggcg gaagtcacca
    13201 ccccagcgcc agctgttctt gccgtgagtg tagaccacgt agtcagaaat cgtgtaggtc
    13261 tggttgcgga tgagctgggg attcgtatct tgcaggccgg agagatcagc gaaggaaagg
    13321 ttcggcaatc cccagtcgaa tggatttgtc gaaacaccag tgattcccaa cccggcgtcg
    13381 acgtcattga gaaacgcata aagattctgg gtgtgcgtcc ggctgcgatt gaaatcgaaa
    13441 cgcaggttgt tggtcagctt gccgaaagtt cgaacatatc ctagaggcac gtcgaaactg
    13501 cgaacactgg tactgccacc gacgctggga aacgggttcg tgatgttggc gctggatccg
    13561 tgatagtgaa aaccgaaatt cacgtcgttg cgggggcctc cgcgcccgcc cccgcgccgc
    13621 ccgcgcgcgg gcgcggcagc tccgatggtg tggttgatgc gcacgttcag gtcgtcactg
    13681 ttgttggtcg tcgaggtcac gaagtgaaaa ttctgaaagt ttcccggcag attcggacac
    13741 ggaatgtatt gcagcagacc tccgggaatc ccccctcccg ttgccgccga ccacgggcac
    13801 gtttgcgaag cgaatccggg aatgacgttt cccgaaaacg gcgcgttggt tgcgggattg
    13861 aaaatctgta cctgctgtcc cgctgccgga cccgacgtat acaagacgct attaaaattc
    13921 ccctgccgct cggcgagagt cggcaccgtt gaaaactgat caaacggatt ttctccgtgg
    13981 cggccgttgt agttggcgaa gaaaaatgtc ttcgtgccgc cgtgatagat cctgggaatg
    14041 ttgagcggac cgccgaccga accgccgaag ctgttctgca gataggccgg cttgaccacc
    14101 ggctggcccg tcagcgaata aggagcggca ttgagagctc cgtcgcccac gccgtagtaa
    14161 atcgatccat gaggatgatt gatatcaaaa ccgcgccggc cggtgatggt catgggacgg
    14221 ccgggaccgc cgccaccaaa tcctccc
//
LOCUS       DASPUL010000123         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1525127, whole genome shotgun sequence.
ACCESSION   DASPUL010000123 DASPUL010000000
VERSION     DASPUL010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525127"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VEJ00_03115"
     CDS             <1..792
                     /locus_tag="VEJ00_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose
                     hydrolase/GNAT family N-acetyltransferase"
                     /protein_id="HXX00178.1"
                     /translation="ELMAQADLAISGAGSTCWELCLMGLPSLLLDVAPNQTRVAHDLD
                     RKGCAIYVGDHDVGVQRIAQQLQCLLRSQELRQSLSQRSRALVDGNGARRVVSAVRGK
                     DAIWLRRARKEDSRQLWEWANDPEVRAASFSSDPIPWDTHDQWFAGKLGEDTSLILIA
                     ENQESIPAGQIRFDGRTDGDWEVDVSIAPSLRGRRLASSLITLGIREIVDENPAARIH
                     AFVKQSNTASLRAFERACFTQIGVEQMRGHAAVHLVYGQPGCLNP"
     gene            789..1508
                     /locus_tag="VEJ00_03120"
     CDS             789..1508
                     /locus_tag="VEJ00_03120"
                     /inference="COORDINATES: protein motif:HMM:NF044825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00179.1"
                     /translation="MKHILTRKAKAYLVVGCKSWNRRIFDEMLRHLPGRWHYIGSPAE
                     LSLDAVEKIAPRYLFFLHWSWKVPDELVGRHECVCFHMTDVPFGRGGSPLQNLIVRGL
                     KTTKLTALRMSREFDAGPVYMKADLSLKGGAEEIYIRAARLSARMIQSMIQREPTPVP
                     QRGEPVNFKRRKPEDSELGAAKCLEELTDFIRMLDAEGYPRAFLQHSGFRFEFSRPAL
                     YDGRIVADVQITRADKKRRTK"
     gene            1505..2197
                     /locus_tag="VEJ00_03125"
     CDS             1505..2197
                     /locus_tag="VEJ00_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HXX00180.1"
                     /translation="MKVLVIAAHPDDEVLGCGATAARLVMEGHEVHFVILGEGMTSRR
                     PERGDTDAAQLATLHQQAQAAAAKVGVKSVVLHKLPDNRLDTLPLLEIVKMVEGHVDR
                     LKPEVLYTHHGGDLNIDHAVIYRAVLTATRPVAGQPVREIYAFEVPSSTEWAFQRLEP
                     PFRPNVFVDVTRTLEAKIAAMSCYESESRKFPHPRSAEALRAIAMRWGSVAGCGAAEA
                     FELVRCLRPQPE"
     gene            2200..>2436
                     /locus_tag="VEJ00_03130"
     CDS             2200..>2436
                     /locus_tag="VEJ00_03130"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HXX00181.1"
                     /translation="MPDQIKVGQRVIGAGHPVFVIAEASANHHQKFDEAVKIVHAAKD
                     AGADAVKLQTYTPDTMTIACNRPEFQIKGTIWEGR"
BASE COUNT          501 a          688 c          785 g          462 t
ORIGIN      
        1 gagctgatgg cacaggcgga tttggccata tctggagcgg ggtcgacttg ttgggaactg
       61 tgcctgatgg gactcccctc cctgttgctt gatgttgcgc cgaaccagac ccgggttgca
      121 cacgatctgg accgaaaagg atgcgccata tacgtgggtg atcacgacgt gggcgtgcag
      181 cgaatcgcgc agcaactgca gtgcctgctg cgatcccagg aactccgcca gtcgttgtca
      241 caacgatcgc gtgcgctcgt ggacggcaac ggcgcaaggc gggtggtatc ggctgttcgc
      301 ggcaaggatg cgatttggtt gcgccgcgct cgcaaggagg acagccgtca gctttgggag
      361 tgggcgaacg atccggaggt gcgcgcggcg tcgttttctt ctgatccgat cccctgggac
      421 acgcacgacc agtggttcgc ggggaaactt ggcgaggata caagccttat attgattgct
      481 gaaaaccagg aatcaatccc ggcgggacag atcaggtttg atggccggac agacggggat
      541 tgggaggtgg acgtcagcat cgcaccaagc ctgcgcggtc gtaggctggc aagcagtctg
      601 atcacgctcg gaatccggga gatcgtagac gagaatccgg cagcccgaat tcatgctttc
      661 gtgaagcagt cgaacacggc gtctctacga gccttcgaaa gggcctgctt cacccaaatc
      721 ggtgtggagc agatgcgtgg ccacgctgcc gtccatctgg tctatggcca gcctggctgc
      781 ctcaatccat gaagcacatc cttactcgga aagcgaaagc ctatctggtg gtcggttgca
      841 aatcctggaa ccgacgcatc ttcgatgaga tgttgcggca tctgccgggg cgttggcatt
      901 acatcggatc acccgccgag ctatctctcg acgccgtcga aaaaatcgct ccacgctacc
      961 tctttttcct ccactggtcg tggaaagtcc cggatgaact tgtcggtcgc catgaatgcg
     1021 tctgcttcca catgacggac gttccgttcg gcagaggcgg aagtccgctg cagaatttga
     1081 tcgtgcgggg cttgaagacg actaagttga cggctctccg gatgagccgc gaatttgatg
     1141 ccggtccggt ctacatgaaa gcggacctct cgctcaaggg aggcgccgag gagatctata
     1201 tccgggcggc gcgattgtcc gccaggatga tccaaagtat gatccagcgt gagccgaccc
     1261 cagtgcccca gcgaggcgag cccgtgaact tcaagcgcag gaagccggaa gacagcgagt
     1321 tgggagctgc caagtgtctc gaagagctaa ccgatttcat ccgcatgctc gacgccgaag
     1381 gatatccccg cgcattcctg cagcattcgg gattccgctt tgagttcagc cggccggcac
     1441 tgtacgacgg acggatcgtc gccgacgtcc agatcacgcg ggctgacaag aagaggagga
     1501 cgaagtgaag gtcctcgtga ttgcggccca tcccgatgac gaagtgctgg gttgcggcgc
     1561 aaccgctgcc cggctggtca tggaaggcca cgaggtgcac ttcgtgattc tgggggaagg
     1621 gatgacctcg cgacggccgg aacgtggcga tacagatgcg gcacagttgg cgactttgca
     1681 tcaacaggcg caggcggcag cggcgaaagt gggggtgaag agcgtggtgc tacacaagct
     1741 gccggacaat cgcctcgaca cgcttccact cctcgaaatc gtgaagatgg tcgaggggca
     1801 cgtggaccgt ttaaaaccag aggtcctgta cacccaccac ggcggtgatc tcaacatcga
     1861 tcatgcggtg atctatcgcg cagtcctgac tgcaactcgc cccgtggccg ggcagccggt
     1921 tcgcgagatc tacgcgttcg aagtgccgtc ttcgacggag tgggccttcc agcggctgga
     1981 acctccgttc cgcccgaatg ttttcgtgga tgtcacgcgc accctcgaag cgaagatcgc
     2041 tgccatgagt tgctatgaaa gcgagtcgcg gaaatttccg cacccccggt cggcagaggc
     2101 gttgcgagcg atcgcgatgc gatggggcag cgtggcggga tgcggggctg ctgaggcgtt
     2161 cgaactggtg cgctgccttc gcccccaacc ggagtaaaaa tgccagatca gatcaaagtc
     2221 ggccagcgcg tgatcggcgc cgggcatccg gtgtttgtga tcgccgaggc ttccgccaat
     2281 catcaccaga agttcgatga ggcggtcaag atcgttcacg ctgcgaaaga tgccggcgcg
     2341 gatgcggtca agctgcaaac ctacacgccc gacacgatga cgatcgcctg caatcgcccg
     2401 gagttccaga tcaaaggcac gatctgggag gggcgc
//
LOCUS       DASPUL010000124         4103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_977771, whole genome shotgun sequence.
ACCESSION   DASPUL010000124 DASPUL010000000
VERSION     DASPUL010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_977771"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4103
                     /locus_tag="VEJ00_03135"
     CDS             <1..>4103
                     /locus_tag="VEJ00_03135"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXX00182.1"
                     /translation="LALAGERGLLMPEIRTAVRTGDTLQPERRAMLKRPPHILVTTPE
                     SLYILLTADRSRAILRDVGTVIVDEIHAVADDKRGAHLALSLERLDALAHARPVRIGL
                     SATQKPIEEVAHFLTGSGRADPVIVNIGHKRKLDLAVEVPPMPLGPIATNEMWDTIYD
                     RLVALVGEHRSTLVFVNTRRMAERIAHQLGERMGEENVAAHHGSLSRKLRLAAEKKLK
                     EGQVRVLVATASLELGIDVGTVDLVVQISSCRAIAVALQRVGRSGHWRGAVPKGRVFA
                     TTRDDLIECAALVRAIKHGDLDRLMIPESPLDVLAQQIVAACSAEEWAEDDMFALARR
                     AYPYRNLERETFDAILEMLAEGIAARRGRYGAYLHRDRVNGKLRARRGARIAAITSGG
                     AIPDNSLYTVVAEPDQVVVGTVDEDFAVESNRGDIMLLGNTSWMIHRIETNAGRMLVQ
                     DAHGAPPTVPFWRGEAPARTEELSLHVGELREKISEMLPRTSPVGFSATQPEVAAAVS
                     WVKEECGLDDSGAEQAIEYVLQGRAVLGAVPTQTTVIAERFFDEGGGMQLVIHAPFGG
                     RINKAWGLSLRKRFCRGFNFELQAAATDNGLNIALAEQHSFPLGDVFHFLQADTVQPI
                     LEQAALDSPIFGTRWRWDANRALALLRFQSGKKVPAQIQRMRSDDLLASVFPDAAACF
                     ENIEGDRQIPDHPLVHEVMKDVLSEAMDVEGLKNLLRGMASGQIRVLAVDTPVPSQFS
                     HEILNANPYAYLDDAPLEERRARAVEMRRVLPESVLEEVGGLDQGAITQVREEAWPDV
                     RDADELHDVLHTLVALPVYEVRDGVYEIPTSRAKDAREMGHPVATLGWERYFERLVGE
                     GRAGIGVVDGHRYWVAAERARSFAALFPGAVFEQTLADVETGEISRDDALLTLVTGWM
                     SHSGPTTASELGRSLGLPASDIEKALLRMEASGTVLRGKFTGAVGARAGAPAPHDSVE
                     LRSTGQPGAAVPTQADEIEWCERRLLARIHRLTVGMLRKQIEPVTPAQFMRWLMRWQH
                     VAPGTQVAGERGTLEVLQQLQGFEIPANAWERHVLARRVADYDHKWLDQLCLTGAVGW
                     GRLSPHPATLDSGAGSGNGSSEGASAPRQRRVIPTSVAPITFFAREEADWLGLGKNPH
                     FSQNRGEVGHPGSETGSGLSYGAQLVHEFLRQRGASFFADIVRGTGKLKAEIETALWE
                     LVAAGLVTADGFDNLRSLIDPKRRAGQGSGRTIRPRHSTGRWALLHADVAVERPRAVE
                     AACWMLLRRYGIVIRDLLARESNLPPWRELLIALRRLEARGEIRGGRFVNGFIGEQYA
                     LPVAVESLRAIRHRAPTGETVTLAAADPLNLVGVVVPGDRVAAIAGR"
BASE COUNT          714 a         1153 c         1503 g          733 t
ORIGIN      
        1 cttgccctgg ctggtgagcg cgggttgctc atgccggaga ttcgcaccgc ggtgcgcacg
       61 ggtgacacgc tgcagcccga gcggcgggct atgctgaagc ggcctcctca cattctggtg
      121 accacgccgg agtcgctcta cattctgctg accgccgata gaagccgggc gattctgcgg
      181 gatgtgggga cagtgatagt cgacgaaatt cacgcggtgg cagatgacaa gcgcggggcg
      241 cacctggcgc tgtcgctcga gcggctggat gcgctggcgc atgcgcgtcc agtgcgcatc
      301 ggtctctcgg cgacacagaa gccaatcgaa gaagtggcgc atttcctgac cggtagcggc
      361 cgtgccgatc ccgtcatcgt caacatcgga cacaagcgga agctcgatct cgccgtcgaa
      421 gtgccgccga tgccgctggg gccgatcgcg acgaacgaga tgtgggacac gatttacgac
      481 cggctggtgg cgctggtggg cgagcatcgt tcgacgctgg tgttcgtaaa cacccggcgg
      541 atggccgagc gtatcgcgca tcagctgggc gagcgcatgg gtgaggaaaa cgtcgcggcg
      601 catcacggaa gtctgtcgcg caagctgcgt ctcgccgctg agaaaaaact caaggaaggg
      661 caggtgagag tgctggtcgc gacggcatcg ctcgagctgg gaattgatgt gggcacggtg
      721 gatctggtgg tgcagatcag ttcgtgccgg gccatcgcgg tcgcgctgca gcgagtggga
      781 aggtcgggtc actggcgtgg agcagttccc aaaggaaggg ttttcgctac tactcgcgac
      841 gatctgatcg agtgcgcggc gctggtgcgc gcgatcaagc atggcgatct tgaccggctg
      901 atgattccgg aatccccgct cgacgttctc gcgcagcaga ttgtggcggc atgttcggcg
      961 gaggaatggg cagaagacga catgttcgcc ctggcgcggc gggcgtatcc gtatcgcaac
     1021 ctggagcgtg agacgtttga cgcgatcctc gagatgctgg cggaggggat tgcggcgcgg
     1081 cgcggccgct atggcgcgta tttgcatcgc gaccgggtca acgggaagct gcgggcgcgg
     1141 cgtggagcgc gcattgctgc catcaccagt ggcggcgcga ttcccgacaa ttcgctctac
     1201 accgtcgtcg ccgaaccgga tcaggtcgtg gtgggaacgg tggacgaaga tttcgcggtg
     1261 gagagcaatc gcggcgacat catgctgttg ggcaacacgt catggatgat tcaccgcatc
     1321 gagaccaacg ccgggcggat gctggtgcag gatgcgcacg gggcgccgcc gacggttccg
     1381 ttctggcgtg gcgaggctcc ggcgcggacg gaggaactgt cgctgcacgt gggcgagctg
     1441 cgcgaaaaga tcagcgagat gcttccgcgt acatcgcctg tcggattctc ggcgacgcag
     1501 ccggaggtgg ccgcagccgt gagctgggtg aaagaggaat gcgggctgga tgattccggg
     1561 gcagagcagg cgattgagta tgttctgcag ggacgcgctg tgctcggcgc ggtgccgacg
     1621 cagacgaccg taatcgccga gagattcttc gatgaaggcg gcgggatgca gttggtgatc
     1681 catgcgccgt ttggcggacg gatcaacaag gcctgggggc tctcgctgcg caagcgcttc
     1741 tgccgcggat tcaatttcga attgcaggct gcggctacag acaacgggct gaatatcgct
     1801 ctcgccgagc agcacagctt tccgctgggc gacgtttttc acttcctgca ggccgatacg
     1861 gtgcagccga tcctggagca ggcggctctg gactccccga tctttggcac gcgctggcgc
     1921 tgggatgcga atcgggcgct ggccctgctg cgctttcaga gcggcaagaa ggtgccggcg
     1981 cagattcagc gcatgcggtc tgatgattta ctggcctcag tgttcccgga tgcggcggcg
     2041 tgtttcgaaa atatcgaggg cgaccggcag attcccgatc atccgctggt gcacgaagta
     2101 atgaaagacg tgctaagcga agcgatggac gtcgagggac tgaagaacct gctgcgcgga
     2161 atggcttccg gccagattcg cgtcctggcg gtcgatactc cggtgccgtc ccagttttcg
     2221 cacgagattc tgaatgcgaa tccgtatgcc tatctcgatg acgcgcccct cgaggagcgg
     2281 cgagcgcgcg cggtcgagat gcggcgggtg ctgcccgagt ctgtgctcga ggaagtgggc
     2341 gggctggacc agggggcaat tacgcaggtg cgggaagaag cttggcccga cgtgcgggac
     2401 gcggacgagt tgcatgatgt gctgcacacg ctggtggcgc tgccggttta cgaggttcgc
     2461 gacggcgttt acgaaatccc cacttctcgc gcaaaggacg cgcgagaaat ggggcaccca
     2521 gtcgcaacat tgggatggga gagatatttc gagaggctcg tcggtgaagg ccgggccggg
     2581 atcggtgtgg ttgacgggca tcgctattgg gtcgcggcgg aacgggcgcg gagttttgcg
     2641 gctttgtttc cgggggcggt gttcgagcaa acgctggcgg atgttgagac cggagagatt
     2701 tcgcgcgacg atgccttgct cacactggtg accggctgga tgtcacattc ggggccgacg
     2761 accgcttcgg aactcgggcg cagccttggg cttccagctt ccgacatcga aaaggccctc
     2821 cttcggatgg aggccagcgg gacagttctg cgcgggaagt ttacgggggc tgtgggcgcg
     2881 cgggcgggcg cgcccgcgcc acacgatagt gtggaacttc gctcgaccgg acagccgggg
     2941 gcggctgtcc ctacacaggc tgacgagatc gagtggtgcg agcgacggct gctggcgcgc
     3001 attcaccggc tgaccgtcgg gatgctgcgc aagcagatcg agccggtcac accggcgcaa
     3061 ttcatgcgat ggctgatgcg ctggcagcac gtcgcgccgg ggacgcaagt cgccggcgag
     3121 cgggggacgc tcgaggtcct gcagcagttg caagggttcg aaattccggc gaatgcgtgg
     3181 gagcgacacg tgctggcgcg gagagttgca gactacgatc acaaatggct ggaccaactg
     3241 tgcctgactg gggcggttgg gtggggacga ctgtcgccgc atccggcgac gctcgattcc
     3301 ggcgcgggga gcggaaatgg atcgtctgag ggggccagcg caccgcggca gcggcgtgtg
     3361 atccccacca gcgtagcgcc gatcactttc ttcgctcgcg aggaagcgga ctggctgggg
     3421 ctcggcaaaa atccccactt ctcccaaaac cggggagaag tggggcaccc gggctcggag
     3481 acgggtagtg gcctcagcta tggggcgcag ctggtgcatg agttcctgcg gcagcgggga
     3541 gcatcgtttt ttgctgacat cgtgcgcggc accggcaagc tgaaggcgga aattgaaact
     3601 gcgctgtggg agctggtcgc cgccggactg gtcaccgccg atggtttcga caacctgcga
     3661 tcgctgattg atcccaaacg ccgggcgggg cagggaagcg gtcgcacaat acggccccga
     3721 cacagcaccg gacgttgggc attgctgcac gctgatgtcg ccgtggagcg gccaagagca
     3781 gtcgaagctg cttgctggat gctgctgcgg cgctacggca ttgtgatccg cgatctgctg
     3841 gcgcgcgaat cgaacctgcc gccatggcgc gaactgttga ttgcgttgcg ccggctcgaa
     3901 gcccgcggcg agatccgcgg cggccgattc gtcaacgggt tcatcggcga gcaatatgcg
     3961 ctgccggtgg cggtcgaatc gctgcgcgcg attcggcatc gcgcgccgac tggcgagacc
     4021 gtgacgctcg cggccgccga cccgctgaat ctcgtcggcg tcgtcgtccc cggagaccgc
     4081 gtcgcggcga tcgcgggtcg att
//
LOCUS       DASPUL010000125         4089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_155830, whole genome shotgun sequence.
ACCESSION   DASPUL010000125 DASPUL010000000
VERSION     DASPUL010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155830"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VEJ00_03140"
     CDS             complement(<1..601)
                     /locus_tag="VEJ00_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXX00183.1"
                     /translation="MNLRIWLQQNNAKKLARRLAVPILAIALAVSFTTYEIAKPASAK
                     AAAAAAPSAAPLDPDSVSALLALDHAMETLAARVTPAVVNVTVTSHAKPDVANEQIPP
                     DMQRFFGQSSPFGFQFFGPRRGQPQIEHGNGSGVVISPDGYIVTNNHVVDSAVDIRVT
                     TSNRRVLKAKLVGTDPLTDLAVIKVDENNLASVPWGDSKA"
     gene            complement(686..2014)
                     /locus_tag="VEJ00_03145"
     CDS             complement(686..2014)
                     /locus_tag="VEJ00_03145"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HXX00184.1"
                     /translation="MVAVFAGLVLRVSPGKSAGARFALWFSALMAIAALPVLGGLAWS
                     SGAGLPAAEMGKTAITLPSSLALYFFGAWAAIAAWSLLRVGMGLWHMHTLRKSCVPID
                     PAELDACLRGTLESGRGARPVSLCTSDRVQTPTAIGLVAPLVVFPRWVLEELPADEVK
                     QILLHELAHLRRWDDWTNLLQKLVRAVLFFHPAVWWIESKVSLERELACDDAVLAVTE
                     QPRAYAECLAHLAEKTLVRRSLALAQAMLGRMRQTSRRVAQILDPNRPRETKHAWRWA
                     VPLVSGLVVACAMLAPSDSQLIGFRDVQPSAGAHAIASSSGSALRPIPAAFQRAAEPT
                     ISKTVARKRTPRIVLARSETINPKPVPRPVENLDAVMADEDPGVEPSLVHVAISDAAS
                     VVQTETVFLVVEHAETGASHQPLYEIQVWRLTVFHPSPHSTSKESPHKET"
     gene            complement(2100..2477)
                     /locus_tag="VEJ00_03150"
     CDS             complement(2100..2477)
                     /locus_tag="VEJ00_03150"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HXX00185.1"
                     /translation="MARNRSAILTEAELRIMNVLWQKGSATVHEVLDAMPAKPALAYN
                     SVLTIIRILEKKGYVRHAKDGRAHVYIPQVNREAATRSEVQRLVSRFFGNSHELLVLN
                     ILEDKSIDAEEMDRLRQLLERSK"
     gene            complement(2726..3289)
                     /locus_tag="VEJ00_03155"
     CDS             complement(2726..3289)
                     /locus_tag="VEJ00_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00186.1"
                     /translation="MSIALEKQTVESNGATAHVLEVVRSAEQELGGLLQRRLEIVARI
                     NALRQLMNDMVTLFGESILDEELRAEMDRRLGGGHRPGFTRACRAVLTGSCGPLRARE
                     ASAELRRRFPEMAGRHKDLTASVTTVFHRFVRYEEARCFLDGEGVRVWQWLVTNRGGA
                     SAQEVAVTFDGDRQPASESPAIAELAP"
     gene            complement(3343..3768)
                     /locus_tag="VEJ00_03160"
     CDS             complement(3343..3768)
                     /locus_tag="VEJ00_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00187.1"
                     /translation="MNRFARVLAGLSITIVLLSVSAAAQYARRVIRVQIPFEFTVSGR
                     SMPAGDYTIVCLAPDRLELRDTQSHAFAWALTLPGESPEKNVSTTLEFSTVDGEHALT
                     EVRVKNETTVYELAARPSPIAVALRQAHDLIHSAGAGNK"
BASE COUNT          818 a         1327 c         1126 g          818 t
ORIGIN      
        1 ccgctttgga atcgccccac ggcacgctcg ccaggttatt ctcgtccact ttgatcaccg
       61 ccaggtcggt cagaggatcg gtgccaacca gtttcgcttt caaaacccgg cgattgctgg
      121 tggtcacgcg gatatccacg gcgctatcga cgacgtgatt gttcgtgacg atgtatccgt
      181 ccggcgagat gacgacgccg cttccgttgc catgctcgat ctgcggctgc ccgcggcgcg
      241 gcccaaagaa ctggaatcca aatggcgagg actgcccgaa gaaccgttgc atgtccgggg
      301 gtatctgctc gttggccaca tcgggtttcg cgtgggaagt tacagtgacg ttgaccactg
      361 ccggagtcac gcgcgcggca agcgtctcca tcgcgtgatc gagcgcaagc agcgcgctga
      421 cactatccgg gtccagcggc gccgccgacg gagccgcagc ggccgccgcc ttcgccgacg
      481 caggtttggc gatttcgtac gtggtgaacg agaccgcaag ggcaattgcc agtatcggca
      541 ctgccagccg cctcgccagt ttctttgcat tgttttgttg caaccagatt ctcagattca
      601 tgatcgtgta tcctctcgaa gattgagcaa ctaattactt agttttcgcg atgaaacgaa
      661 ctcccgatct ctcttccctt cctttctaag tttccttgtg gggagattcc ttgctggtgg
      721 agtgcggcga aggatggaac acagtcagac gccacacttg gatttcataa agcggctggt
      781 gcgaagcgcc ggtctccgcg tgctccacaa ccagaaaaac tgtttctgtc tgcaccaccg
      841 aagcggcgtc ggaaatcgcc acgtgaacca ggctcggttc gacgccgggg tcctcgtctg
      901 ccattacagc atcgaggttt tcaaccggtc gcgggaccgg cttgggattg atggtctcgc
      961 ttctcgccaa cactatgcgg ggcgtgcgct tccgcgcaac ggttttcgat attgttggct
     1021 cggcagctct ttggaacgct gcaggaatcg gacgcagcgc cgaaccactg gacgaagcga
     1081 ttgcatgagc cccagcgcta ggttgcacat cccggaagcc gatcagctgc gaatcgctgg
     1141 gggcgagcat ggcacaggcg accacgagtc ccgacaccaa cggcacagcc catctccagg
     1201 catgcttcgt ctctcgcggc cgattcggat cgaggatctg cgccactcgc cttgacgttt
     1261 gccgcatacg tccgagcatg gcctgcgcca gcgcgaggct ccggcgtaca agagttttct
     1321 ccgccagatg cgccaggcac tccgcatagg cgcgaggctg ctccgtcacg gccagaacgg
     1381 catcgtcgca ggccaactca cgctccagag acaccttgct ttcgatccac cataccgccg
     1441 ggtgaaagaa aagtaccgcc cgcacgagct tctgcaagag gttcgtccaa tcatcccacc
     1501 gccgcagatg cgccagttcg tgcagcagaa tctgtttcac ctcgtccgcg ggcaactcct
     1561 cgaggaccca acggggaaaa accaccagag gcgcgaccaa tccgattgcc gtgggagtct
     1621 gcactcgatc cgatgtgcaa agcgacaccg gcctcgcacc gcggccgctc tccagcgtgc
     1681 cgcgcaggca tgcatccagt tcggcgggat cgatgggcac acaactcttg cgcagcgtgt
     1741 gcatatgcca caatcccata ccgactcgga gcagagacca cgccgcgatt gccgcccacg
     1801 cgccgaagaa atacagagcc agcgaactgg gcaaagtgat cgccgtcttg cccatctcgg
     1861 ccgcaggcaa acccgcaccg gatgaccacg ccaggccgcc caacacaggc aacgctgcga
     1921 tcgccatcag cgccgagaac cacagcgcga acctcgcgcc tgcactcttc ccgggcgaga
     1981 cccgcaacac caggcctgcg aacacagcga ccattgtgcc ttctaacaaa cagtccacca
     2041 ttcgcactgt ggatgtctga gcaatggcat gtagttcgaa cgccccagtc atcagtggct
     2101 cacttgctcc tttccagcag ctggcgcagg cgatccattt cctccgcgtc gatactcttg
     2161 tcttccagga tgttcagcac cagcaattcg tgggagttcc cgaagaaacg gcttaccagc
     2221 cgctgcacct ccgaccgggt cgcagcttcg cggttcacct gcggtatgta gacatgcgcg
     2281 cgcccgtctt tcgcatgtct gacgtatccc tttttctcca ggatgcgaat gatggtaagc
     2341 acggagttgt aggctagcgc cggcttcgcg ggcatagcgt ccagcacctc gtgcaccgtc
     2401 gccgagccct tctgccagag cacgttcatg atgcgcaact cggcttccgt gaggatggcc
     2461 gacctgttcc gagccaatct cgccctccct atccttgcct cccggtctcc tatagagacg
     2521 tgggagcaaa ggaaacgtca actaatttat tagttgccag caagagacac cgggttgccc
     2581 ttcccatccg gcagtacttc ggttggtctt aacctgctgt ccgcctgaga gataaccggt
     2641 gagaacacgg tcactcgcgc cggtctgaag cgtaaaggta gtcagcttct atcggcgaga
     2701 ggcgcatcta cgatccgagg agagattacg gggccagttc cgctatggcg gggctttccg
     2761 atgcaggttg ccggtcaccg tcgaacgtga cggcgacctc ctgagcactc gcgcccccac
     2821 gattcgtcac caaccactgc cagaccctga cgccctcgcc gtcgagaaag cagcgggctt
     2881 cttcgtacct gacgaaccga tgaaacaccg tggtcacaga cgccgtcaga tccttgtggc
     2941 ggccagccat ttccgggaat ctgcgccgca actcggcgct cgcctcccgc gcgcgcagcg
     3001 gaccgcaaga acctgtcagc acggcgcgac aggcccgagt aaaacccggg cgatgccccc
     3061 cgccgagtcg ccgatccatc tccgcccgca gttcctcgtc aagaatcgac tcgccgaaca
     3121 gcgtcaccat gtcgttcatg agttgccgga gggcattgat ccgcgccaca atctcgagcc
     3181 gccgctgaag aagcccgccc aactcctgtt ctgctgagcg gaccacttcc aggacatgcg
     3241 ccgtcgcgcc attgctctcc accgtctgtt tctccaaagc aatgctcatt gcgccctcct
     3301 tccccatacg tcctgcggac gttgcgcgca acccgtcgtc cactacttgt ttcccgcgcc
     3361 tgcggagtga atcaggtcat gcgcttgccg cagcgcgacg gcaattggcg acggtcgcgc
     3421 ggccaattcg taaaccgtcg tctcgttctt tacccgcacc tctgtcagcg cgtgctctcc
     3481 atccacggtc gagaactcaa gcgttgtcga aacgttcttt tccggggatt cgcccgggag
     3541 cgtaagcgcc cacgcaaacg catgcgactg cgtgtcgcgc aattccagcc gatcgggtgc
     3601 cagacatacg atggtgtagt ccccggctgg catgcttctg ccactgacgg tgaattcaaa
     3661 aggaatctgc acccggatga cgcgccgcgc atactgcgct gccgcggata ccgagagcaa
     3721 aacgatagtg attgataaac ctgcgagaac tcgggcaaag cgattcatat ctgtctcctg
     3781 taatgatgca ttctggattg agccccacgg cgccgccctt gcttgcatta ctctgcgcaa
     3841 actttttttg cgacgctatc cgcttcttgc gatcaatgtc acagagtgcg ctttcttcat
     3901 tggacgcagt tagtggcaca acgatcattg tttgttctca ttgcacgcat gaggcgattt
     3961 catatttacc gccatcaatg cagtccattg gcgaaatggc atgcagacga aacagtcagg
     4021 agaacagcgg gaaggagacc gttccgatgc cggcaagaaa cgacgtgagg ctcgaaatcc
     4081 gccacttgc
//
LOCUS       DASPUL010000126         4193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1525752, whole genome shotgun sequence.
ACCESSION   DASPUL010000126 DASPUL010000000
VERSION     DASPUL010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525752"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VEJ00_03165"
     CDS             complement(<1..225)
                     /locus_tag="VEJ00_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00188.1"
                     /translation="MTWVKICGMTNLEDALIAVEAGADAVGFVFYEKSPRCVSVASAK
                     RIVEKLPQQTDKVGVFVNEEEETICSVADEV"
     gene            complement(313..1119)
                     /gene="trpC"
                     /locus_tag="VEJ00_03170"
     CDS             complement(313..1119)
                     /gene="trpC"
                     /locus_tag="VEJ00_03170"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HXX00189.1"
                     /translation="MSTFLGQIVAATRTRVVEAKTRADRRRLEGMAETHVPRGFRRAL
                     QSKGSAGVAVIAELKKASPSKGLIRADFRAADLARELEEAGAAALSVLTDEEYFQGSL
                     ANLREASAAVRVPCLRKDFIVDEFQLLEARANSADAVLLIVAALSSQELQTLTSAARS
                     CGLDVLCEVHDAEELKRALDAGCNLIGVNARDLRTFEVNLETAFALAEKFPAGVVRVA
                     ESGIHSADDVARLRAAGYDAFLVGESLMRAEHPGEALRELLAGAEYRVAR"
     gene            complement(1223..2152)
                     /locus_tag="VEJ00_03175"
     CDS             complement(1223..2152)
                     /locus_tag="VEJ00_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00190.1"
                     /translation="MIERISYRGWENCWKLSNDAVELIVLADVGPRIVWYGFRGGENQ
                     FHEVEQDAGRTGGVEFRLYGGHRLWASPEVARTYFPDNVSVQVQQLGSVVRFTAPVED
                     APPGTGLQKEIEVELESAGSGVRLTHTIANRGSARTELAVWTPTMMRAGGRAILPLPE
                     RHAMDTDHVLPVGVFGVWSYTDFADPRWNLGTEFVQLQQTAEPAGRFREQMGGIFNSA
                     GWGAHYRQGTLFVKRAAVIAGARYPDGGCNFELFTNPDFLEVETLGPLVELNPGETAE
                     HVERWWLFGDVPEGQGEEWIRREIVPRAGRTRI"
     gene            2395..3423
                     /locus_tag="VEJ00_03180"
     CDS             2395..3423
                     /locus_tag="VEJ00_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00191.1"
                     /translation="MTRINALRNTKETRSSLSWGARVESWGKNAGALVFAAVLIVCSV
                     TVGCSSDKPKPVISENQMPITQAPSPATPTPAPAMQAENKPAPKKVVHRKPATVNYAD
                     KTYGVTFEYPRRYAIETGNAAQDLVASNPLSMNFVQPGGVALAAVELPETGFANTDFS
                     SAFFNVSVNKNLTAEQCNEFAVPLPKVVEKTITDASSSAQNSDAKAAAGSTETASATV
                     AGATTTPPVTSSDAQPIAKSDSDQPGSKLLLGDMELRATEAVSGEGSRQSDSKYFHVF
                     QNGACYEFALNVNTMASEDGLIKHVDRDKVFSRLEAILGTVKINPVTPEVTAEAPAPA
                     PAPETPAQ"
     gene            complement(3494..3952)
                     /locus_tag="VEJ00_03185"
     CDS             complement(3494..3952)
                     /locus_tag="VEJ00_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXX00192.1"
                     /translation="MNSLRNGALLLCLLAAFPLLGRAQDRDKPDAAAVRSLELKWTES
                     YKQRNIDILSSLLAEDFVITIEDGSVYSKAGYISHSADASTHVEVAELSDLKVRVHGP
                     VAIVTGAYHEKGESNRRPYEYHDRLTDVWMKAGGKWQVVASHYSVPFKNN"
BASE COUNT          831 a         1436 c         1131 g          795 t
ORIGIN      
        1 gacctcatcc gctactgagc agatcgtttc ttcctcttca ttcacgaaca ctcccacctt
       61 gtctgtctgc tgcggcagtt tctcgacaat tcttttcgcg gacgcgacac ttacacaccg
      121 cgggctcttc tcataaaaca cgaaccctac tgcatccgcg ccggcttcta cggcgatgag
      181 agcgtcctcc agattcgtca tcccgcaaat ctttacccaa gtcataagag cagggggcag
      241 ggatcagtgg tcagggccca gcgaaactcg tcgggtgccc cacttctcgc gctctttgcg
      301 agaagtgggg ggctaccgtg caacccgata ttcggcaccc gcgagcaact cgcgcagcgc
      361 ctctcccggg tgctccgccc gcatcagcga ctcgcccacc agaaacgcgt catagcccgc
      421 ggcgcgcagc cgcgccacgt cgtctgcgga atgaatgccg ctctcggcta cccgcaccac
      481 gccggcggga aacttctccg ccagagcgaa tgcggtctcc agatttacct cgaacgtccg
      541 caggtcgcgc gcattgactc cgatcaggtt gcatcccgca tccagcgccc gtttcaactc
      601 ctcagcatcg tgtacttcgc acagaacatc gagcccgcag gagcgcgccg ctgacgtgag
      661 tgtttgcaat tcctgcgaag acagcgccgc tacgatcagc aacacggcgt cggcggaatt
      721 cgcccgcgct tccagcaact ggaattcatc cacgatgaaa tccttgcgca ggcagggaac
      781 cctgaccgct gccgaagcct cccgcaggtt ggcgagcgat ccttgaaagt actcttcgtc
      841 ggtcaagact gagagcgctg ccgcacccgc ctcttccaat tcccgcgcca aatctgccgc
      901 acgaaaatcg gcccgaatta accctttcga cggcgaagcc ttcttcaatt ccgcgatcac
      961 cgcgacgccg gcgctgccct tcgactgcag agccctccga aacccccgcg ggacgtgtgt
     1021 ttccgccatc ccttcaagcc ggcgccgatc tgcgcgggtt ttggcttcca ccacccgtgt
     1081 ccgcgtcgcc gccacaatct ggccaagaaa agtcgacata gagaatcttt gattatacgg
     1141 gacatatcag gacgtctcgc catcccaggc ccccgtaagg cttcgcggga gccagctgcg
     1201 cgctcgctcc aggcacttcg gctcaaatcc tcgtgcgtcc tgcccgcggc acgatttccc
     1261 gccgaatcca ctcttcgccc tgaccttcgg gaacatctcc gaacagccac catcgttcga
     1321 cgtgctccgc cgtttctccc ggattcaact cgacgagcgg ccccagcgtc tccacttcca
     1381 ggaaatccgg attcgtgaac agctcaaagt tgcagccgcc gtccggataa cgtgcacccg
     1441 cgatgaccgc cgctcgcttt acgaacaacg tcccctgccg gtagtgcgct ccccaacccg
     1501 cggagttgaa gattccgccc atctgctcgc gaaatcgtcc cgccggctcc gctgtctgct
     1561 gcaactgcac gaattccgtc cccagattcc agcgcggatc ggcaaagtcg gtatacgacc
     1621 acacgccaaa gactccaacc ggcagcacgt ggtctgtgtc catcgcatga cgctccggca
     1681 gcggcaggat cgcccgcccg ccggcgcgca tcatggtagg agtccacact gccaactccg
     1741 tccgggcgct gccacggttg gctatggtgt gagtcagccg cacgcccgac cccgccgatt
     1801 cgagttcgac ctcgatctcc ttttgcaaac ccgtgcccgg cggcgcatcc tcaaccggcg
     1861 ccgtaaatct cacgacagac cccagctgtt gaacctgcac ggaaacattg tcggggaagt
     1921 aggttctggc cacttccggc gaggcccaca ggcgatgccc gccgtaaagg cggaactcca
     1981 cgccaccggt ccgacccgca tcctgctcga cctcatgaaa ctgattctcg cctcctcgaa
     2041 acccatacca gacgatccga ggccccacgt ccgccagaac gatcagttcg acggcgtcgt
     2101 tcgatagctt ccagcagttc tcccatcccc ggtaagagat gcgttcgatc atgcctcgcc
     2161 cctggcaggc cggccgttat ttcagtttcg cgtaccgccc tgcaaaagtt gtcaagactt
     2221 cgtcatgcgc caagcatacc tgtggtgctc cgcccgtgtc ccggcgccca catcgtgttt
     2281 cgcgggctac atcccgggtt cgtgtgagtc cgctaactgc cttcaaatca acaccgcacc
     2341 gctggcacag gccttgcatg gacgatggca gccgtatgtc ggcaggaggt gaccatgacc
     2401 cgcatcaacg cattacgcaa cactaaagag actcgcagtt ccctatcctg gggtgcgcga
     2461 gtcgagtcct ggggcaagaa cgccggcgcg ctcgttttcg ccgccgtcct gatcgtttgc
     2521 tccgtaaccg tcggctgttc cagcgacaag ccgaagcctg tgatttccga aaaccagatg
     2581 cctataacgc aggctccgag ccctgccacg cctacgccgg caccggccat gcaggccgag
     2641 aacaagccgg ctccgaagaa ggtcgtgcac cgaaaacccg cgaccgtgaa ctacgcggac
     2701 aagacctacg gcgtgacctt tgagtatccg cgccgctacg ccatcgagac cggcaatgct
     2761 gcccaggacc tcgtggcctc caatccgctt tccatgaact tcgtccagcc cggtggtgtt
     2821 gcgcttgccg ccgttgaact gccggagacc ggcttcgcca acaccgactt ctcgtcggca
     2881 ttcttcaacg tgagcgtcaa caagaatctc accgccgaac agtgcaacga gttcgccgtg
     2941 ccgctgccta aagtagtgga gaagaccatt accgacgcgt cctcgtcggc tcagaacagc
     3001 gatgccaaag ccgcggccgg gtccacggag accgcatccg cgacagtcgc cggtgcaacc
     3061 acgactccac cagtcacgtc gtctgacgcg caaccaatcg cgaagtccga ctccgaccag
     3121 ccgggctcga agctcctgct cggcgacatg gaactgcgcg ccaccgaagc cgtctccggc
     3181 gaaggctcgc gccagagcga ctcgaagtac ttccatgtct ttcagaatgg agcctgctac
     3241 gagtttgcgc tgaacgtcaa cacgatggcg tccgaagacg ggctgatcaa gcacgtcgac
     3301 cgcgacaagg tcttcagccg cctcgaggcg attctcggga ccgtgaagat taacccggtg
     3361 acgccagaag tcactgccga agcgcccgcc ccggcgcccg ctccggaaac cccggcgcag
     3421 taaggaggat gacgcagaac tgagtgaagg tgacaggagg ccctgtctcg ccacccggcg
     3481 ggatagggct tttctaattg ttcttgaaag gtacgctgta gtgcgaagcc accacctgcc
     3541 acttgccacc ggctttcatc catacgtcgg tcaggcggtc gtgatattcg tagggcctcc
     3601 ggttggattc acccttctcg tgatatgccc cggttacgat cgcgaccggg ccgtgtactc
     3661 gcaccttcag atcggaaagc tcagccacct ccacgtgggt agaagcgtcg gcgctgtggc
     3721 tgatgtagcc ggctttgctg tacacgctcc catcctcgat ggtgatgacg aagtcctcgg
     3781 ccaacagtga agagagaata tcaatgttgc gctgcttgta ggattcggtc cacttcagct
     3841 cgagggagcg caccgcggcc gcatctggct tgtctctatc ctgggcccgg ccaagcaagg
     3901 gaaaagcagc gagcaggcag aggagcaaag cgccgtttct gagagaattc atccctacta
     3961 tgtcgcacag tcagtcgcct gcggcaagtt ccttatgaaa tccgcgcatc cggtaaatcc
     4021 gggttgcgga tgttgcagtt tcggaactgt atctcaggac tgccgcctcc cagccgttct
     4081 actagacttg ggtagggagc ggtgcgattc gcaaatcgcg tcggggctat ggccgaatcg
     4141 ttagcagaat tctttgtaga gaactttcag gcgcgccgca acgagtgcgc cta
//
LOCUS       DASPUL010000127         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1252184, whole genome shotgun sequence.
ACCESSION   DASPUL010000127 DASPUL010000000
VERSION     DASPUL010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252184"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="VEJ00_03190"
     CDS             complement(<1..1506)
                     /locus_tag="VEJ00_03190"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX00193.1"
                     /translation="MEQQNHNSSAAPNVYWRVEGSLLDITVVEPVAFFTWNAQTFSER
                     FRRRGLIFVMALLRPFLYSTNRKFATRVVHTALRGISRDRLDLLGEEFFQYRLKPRLK
                     ERGVQKVRELVQAGAEVVLVSQGLEHVMRPLAQHLGVKRIVANRLDFRDGMATGRLLD
                     PVIRPRGAFARIREENPDGRRAPKTLARHLDISLETLRAAAVPAERQVTVPARPIVHF
                     DGQRQAPGFSVRRAFAGKHVMLIGVTGFIGKVWLVNTLLDLSGVERIYLLIRRQKTNP
                     AERRFEKMVEDSPVFDPLFERYGNGLANFLRKKIEVVEGDVTESGLGLNPETARYLQK
                     NLDLIINSSGLTDFNPDLRDALATNTDAAVNILEFVRSSDHAGLLHLSTCYVAGEQDG
                     RVSERLVPNYNPRGLIDFDAEQEWRSLHELIKDAEARAETEEVTSGLRMQARAKEHAA
                     KNLQGAALENQVRKNRVRWLKTFLTEAGTRRAKELGWPNTYTLTKSLAESLI"
     gene            complement(1644..>2778)
                     /locus_tag="VEJ00_03195"
     CDS             complement(1644..>2778)
                     /locus_tag="VEJ00_03195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX00194.1"
                     /translation="GRAAGMFFAGYFFFQVPSNLILDKVGVRRWISALMVAWGVISCL
                     MIFIRGPVSFYGMRFLLGAAEAGFFPGMILYMKRWFPARARARAVAWFMTANPLAGLF
                     GSPISGALLGIHSASLAGWQWMFLIEGTPAILLGLTVLVTLAERPDDARWLRDDERDW
                     LQARLAEEGTEESAPNWGSVWEVVFSLRIWMLALVYFAVPTTMYGVTLWLPSVIRALS
                     GLSYVWTGIVAALPFAVAVVLSVLGGIHSDRTGERRWHTAIPAFTGAAALLIAGYGSS
                     PALVIACFGLSMACAQTMSGPFWALATSRLEGLSAPAGIAVINSLANLGGYFGPDIIG
                     LFRTVNGGFRGGLLAIGATLIMSGSMALALGRASAPKGAAGKA"
BASE COUNT          571 a          884 c          857 g          466 t
ORIGIN      
        1 gatcagcgat tcggccagac tcttggtgag agtgtaggta ttcggccagc cgagttcctt
       61 ggcgcgccgg gttccggctt cggtcaaaaa cgtcttgagc cagcggactc ggttcttgcg
      121 gacctgattc tccagcgccg caccttgcag attctttgca gcgtgttcct tggcccgtgc
      181 ctgcatgcgt aggccagagg tcacttcttc ggtttccgct cgcgcctcgg cgtctttgat
      241 caactcgtgg agcgaacgcc attcctgctc ggcgtcgaag tcgatcaagc cgcgcgggtt
      301 gtagtttgga acgagccgtt cgctgacgcg cccatcctgc tcgcccgcca cgtagcaggt
      361 ggagaggtgc agaaggccgg cgtgatccga cgatcgcacg aactcgagga tgttgacggc
      421 tgcatcggtg ttggtagcga gagcgtcacg cagatcggga ttgaagtcgg tcaggccgga
      481 gctgttaatg atcaggtcga gattcttctg cagatagcgg gcggtctcgg ggttgaggcc
      541 aagtcctgac tcggtgacgt cgccctctac gacttcgatc ttcttccgca ggaagttggc
      601 cagtccattt ccgtagcgct cgaacagggg atcgaaaacg ggtgaatcct ccaccatctt
      661 ctcgaaacgg cgctccgcgg gattcgtctt ctggcgtcgg atgagcagat agatccgttc
      721 cacgcctgat agatcaagga gcgtattgac cagccagacc ttgccaatga atccggtgac
      781 gccgatcagc atcacgtgct ttccggcaaa agcccggcgc acggaaaagc cgggggcctg
      841 ccgctgcccg tcaaaatgta cgatgggccg cgccggcacc gtcacctggc gctcagccgg
      901 aactgcggcc gcccgcagcg tttccagcga aatgtcgaga tgccgtgcca gagtcttggg
      961 agcgcggcgg ccgtccggat tctcctcgcg gatgcgggca aaagcgccgc gaggccgaat
     1021 gacgggatca agcaatcgac ccgtcgccat accgtcgcgg aagtccagcc ggttggcaac
     1081 gatgcgcttc accccaagat gctgagccag cgggcgcata acgtgctcca ggccctggct
     1141 caccagcact acctcggccc ccgcctgcac cagttcccgc actttttgca cgccgcgctc
     1201 cttcaaacgc ggcttgaggc gatattgaaa gaactcttcg ccgagcaggt cgaggcgatc
     1261 gcggctgatt ccgcgcagcg cggtgtgcac cacccttgtt gcaaacttgc gattcgtgga
     1321 atagaggaac gggcgcaaga gcgccatcac gaagatcagc ccacggcggc ggaagcgctc
     1381 gctaaaggtc tgggcgttcc aggtaaagaa cgctaccggc tcgactaccg tgatgtcgag
     1441 caggctgccc tccacacgcc agtaaacgtt gggggccgca ctggaattgt ggttctgctg
     1501 ttccaagact cgccttcgcc tcgacggaag cctcccggga caggcaggca gacgaaactc
     1561 ttattgtaaa caatggaagg gagatcgcga agcagggtga ggccgaaagc acctttttga
     1621 cacacccccg agggccgagg ctcctaggcc ttcccggcgg cgcccttcgg tgcggatgcc
     1681 cgaccgagcg ccagcgccat gctgccgctc atgatcaggg tggcgccgat tgccaggagg
     1741 cctccacgaa atcctccatt gaccgtgcgg aacaacccga taatgtcggg cccaaaatag
     1801 ccccccagat ttgccaggga gttgatgacg gcgatgccag cgggcgccga aaggccttcc
     1861 aggcgcgaag ttgccagcgc ccagaaggga cccgacatcg tctgagcaca cgccatgctc
     1921 agtccaaaac aagcgatcac cagcgccggc gaggatccgt atccggctat cagcagcgcc
     1981 gcggctccag tgaaggcggg tatcgccgtg tgccagcgcc gctccccggt gcggtcggag
     2041 tggatgccgc ccaggacgga caagactacc gccacggcga atggaagggc ggccactata
     2101 ccggtccaca catagctcag cccggagagc gcccggatca cgctgggcag ccacagcgta
     2161 actccataca tcgtggtagg gacggcaaag taaaccagag ccagcatcca gatccgcagg
     2221 ctaaaaacca cctcccaaac acttccccaa ttcggcgccg attcctcggt gccttcctcg
     2281 gccagcctgg cctgcagcca gtctcgctca tcgtcgcgca gccacctggc gtcgtcgggg
     2341 cgctccgcca gggtcacgag caccgtcagt cccagcagga tcgccggggt accctcgatg
     2401 aggaacatcc actgccagcc cgcgagagaa gcagagtgga tgcccagaag agctcctgaa
     2461 attgggctgc cgaacaatcc ggcgagaggg ttagccgtca tgaaccaggc caccgcgcga
     2521 gcccgggcgc gggcaggaaa ccagcgcttc atgtacagga tcatgccggg gaagaatccc
     2581 gcctcagcgg cgcccagcag gaagcgcatg ccgtagaaac tgaccgggcc gcggatgaag
     2641 atcatgaggc acgagatcac gccccacgcc accatgagcg ccgaaatcca gcgccggacg
     2701 ccgaccttgt ccagaatcag gttgctggga acctgaaaga agaagtagcc ggcgaaaaac
     2761 atgccggctg cgcggcca
//
LOCUS       DASPUL010000128         8567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_430040, whole genome shotgun sequence.
ACCESSION   DASPUL010000128 DASPUL010000000
VERSION     DASPUL010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430040"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VEJ00_03200"
     CDS             <1..249
                     /locus_tag="VEJ00_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00195.1"
                     /translation="ASDPKRVARFMADRGLWSYDITLQGLRELPYGKWREIDVADSMR
                     FWAVRLHDVGAIKTSPQKIIDQGTDLRFLNELKKELKA"
     gene            664..1674
                     /locus_tag="VEJ00_03205"
     CDS             664..1674
                     /locus_tag="VEJ00_03205"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HXX00196.1"
                     /translation="MSKVFFPAIWMFLAASAFAASFDCTSTANSVEKFVCNNPTISSL
                     DSRLGTIYVNDMSKASDEQRERLVREQRHWLKFSRNSCRDETCFKHAYWSRLSELETF
                     FNRESLPKSEAERAETFKQTIASSPLFLTDWSSPQAFCNKIFGDLKQMKGVTFLEPVA
                     RAQSYEDEALDPWKRECHGKAPLHFSYRFCGRNISPDDNKRQGGLKNCTAGYGLPPFK
                     IFELPPLTGSTDKRYVFYSESDYGPMNGGNAKLNGGGASGYQQIDIKECRAQVGYSVP
                     TSRSEPDGTNESAIIEYEGRYYILTVARSFNAYWVTVNTGSPMSGQCVWSPVKPKPQQ
                     SQ"
     gene            1964..2386
                     /locus_tag="VEJ00_03210"
     CDS             1964..2386
                     /locus_tag="VEJ00_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXX00197.1"
                     /translation="MASIGLKVRDVMSRDVHTVKRNDELAIADALMKQERVRHLPVLG
                     EDGEVCAVVSQRDLFRGALLRALGYGARAEELMLKQVPVKDAMSSEIHTTAPDTPVAD
                     AARVMIERRIGCLPVVENGRLVGIVTETDFVRLVAEGR"
     gene            complement(2442..3275)
                     /locus_tag="VEJ00_03215"
     CDS             complement(2442..3275)
                     /locus_tag="VEJ00_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00198.1"
                     /translation="MLATDSSGLRSPLLLAVGIWLVGIVNGWADEAPTYGRLDSTREA
                     ASIFYGCQQKRDKFLDCELNLIIVSKKAKPEDLSTRLNQAREAFRSDGVEWSSEYCEW
                     FTDFVDILEGRKTPPRGYDSKAVAEAQKKLRETAESEKKDLLNWEKANVEFCKSKTED
                     NWLNLVRLEYENDSRSCIVSARTFIDRFRYMRDSGVGVWVTESSPQGPCGIVQLARFE
                     PELRGNFVFWKYVVRATVTNPEGTTLEGKSCKQEELGEYLYDWRTKAHSLGCEYIDFQ
                     W"
     gene            complement(3287..3613)
                     /locus_tag="VEJ00_03220"
     CDS             complement(3287..3613)
                     /locus_tag="VEJ00_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXX00199.1"
                     /translation="MRPFVWILIASTVPALAVAGPGEDFDRGYKLADAKGCLECHALG
                     YSYIGPSFSAIAQRYRRNPEYRERLPYVIRGGSAGHWGERFVMWPQASLSDAEVRQLV
                     DWVLSQ"
     gene            complement(3663..3899)
                     /locus_tag="VEJ00_03225"
     CDS             complement(3663..3899)
                     /locus_tag="VEJ00_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00200.1"
                     /translation="MRSWADLLYQVTRMEKNAGQDWSSVEIRDVAAYLNELFYKMPCP
                     EQGCEGPEASTGGSTLLAESKPANSRRSTPRIDP"
     gene            4509..4700
                     /locus_tag="VEJ00_03230"
     CDS             4509..4700
                     /locus_tag="VEJ00_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00201.1"
                     /translation="MAWFNMRNALIARVPLLVVNGQQDSRFLIHNPVLGAPNTRLAET
                     ATKYSYEVTSVVLVIDREP"
     gene            5046..5750
                     /locus_tag="VEJ00_03235"
     CDS             5046..5750
                     /locus_tag="VEJ00_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00202.1"
                     /translation="MFWEAIIRGLGVLIHWETYIAVVLYFVIFVGPLVLVGLAVQRSE
                     VGTGLGGCLMTFVMPVLQTFAIFVFVLTLFPIILGVGNGAAWTLPWVLGGQDPWLMAR
                     MVVVLFIAALLMAFVPLVGAWESLHTAVLGSIALTFVIRMLDSPGSVYSHIALWPGLW
                     FFLGLVAVGGLMAWVGRLVAVGLGVLVSAGLARVLKNSTEGIATLLMFPMAGIFGFLP
                     MFLYGAYLAAQIHSSG"
     gene            complement(5835..6491)
                     /locus_tag="VEJ00_03240"
     CDS             complement(5835..6491)
                     /locus_tag="VEJ00_03240"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXX00203.1"
                     /translation="MERILEPEIMGDEQQALAYAKADFSISNQWYVDHLLADYPDRLG
                     SVLDIGCGPGDVMLRLAAARLTTRITAIDGSHAMIELVRKAVQSAGFQERITPLQGYI
                     PGLPLPEHSFDAILSKDLLHHLPDPMVLWREAKRLGRPGAAIYVMDLMRPDSPQAARN
                     IVETVAPNELPILKEDFYASLCAAFTPEEVKKQVNRSGLPLQVTPVSDRHMVIQGVLA
                     "
     gene            6665..8038
                     /locus_tag="VEJ00_03245"
     CDS             6665..8038
                     /locus_tag="VEJ00_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012553159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HXX00204.1"
                     /translation="MMGQQSQVQHQLFYAFNLDSLVPPDHLLRRIDRFLDLGGLRRHL
                     ARFYSHTGRPSVDPELLIRMLIVGYCFGIRSERRLCEEVQLNLAYRWFCRLNLEDAVP
                     EHSTFCKNRHGRFRASGAFRRLFDEVVRSRMAAGVVKGEGFAVDASVVAADASGQRGV
                     PGNEPVNWKDPELSTRAVREYLEALDNESLAQTLPKNISLTDPQSRWTAAPGGPAFYA
                     YSTNYLVDTEHGVIVDVEATPANRTAEVESAKTMVDRVEERFKLVPERLIGDTAYGTA
                     SMLGWMVEDKGIEPHVPLWDKTQRNDNTLSSSEFQWNEQAYEYRCPQGHALRSQRRAF
                     KKPRTHVTKANTIIYRSSQADCATCPMKERCCPNTSVRKIARSIYESARDVARRVRTT
                     VQYRRSRYLRKKVEILFAHLKRILKLDRLRLRGLSGARDEFTLAAAVQNLRRLATLIP
                     QGPPAPA"
     gene            complement(8345..>8567)
                     /locus_tag="VEJ00_03250"
     CDS             complement(8345..>8567)
                     /locus_tag="VEJ00_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HXX00205.1"
                     /translation="ALRGINAAVVGLLLAALYDPVWTSAVKSRADFGLALAAFTLLMF
                     WKVSPVIVVAFAAAGGWLLALGFSLSALF"
BASE COUNT         1845 a         2451 c         2447 g         1824 t
ORIGIN      
        1 gccagtgacc cgaagcgcgt tgcacgcttc atggccgatc gagggctgtg gagctacgac
       61 atcacgctcc aaggactccg cgagctgccc tacggaaagt ggcgagagat tgacgtggcc
      121 gattcaatgc gcttctgggc ggtgcggttg catgacgttg gggcgatcaa gaccagtccg
      181 cagaagatca ttgatcaagg caccgacctt cggtttctta atgagctgaa gaaggaactt
      241 aaggcatagc gaggctgggc cgggagtggg gtgagttcca acgtccgctt ctctaaagtg
      301 ccaccgtccg caacgggtcg accgcgccag cgaacggccg ctctcgggaa ctaagccctc
      361 agctcggacc cccgagcaac ccccgctcgt cgcaacgcgc aaggaagtct tactgctgga
      421 ctcgatccca gggagtctcg gggctgccca tccccgtcgg ctcgaccggc agagggcgcg
      481 taagcggaca ttcgcggact tctctgatcg gcccaaaagc ggtactagcc aacattcccc
      541 taaagcggtc attgtcagtc cgatctcagc cacgcccacc ttaagtttgc ctctgagcgc
      601 gcgtaaatca ggtcacgatt ccagagttgc aggatcggca ttcaacctga ggcgttgtga
      661 gaaatgagca aggtgttctt ccctgccatc tggatgttcc ttgctgcctc ggcattcgct
      721 gcaagtttcg attgcaccag cacagcaaac tcggtagaaa agttcgtctg caataacccc
      781 acgatctctt cattagatag tcggctgggc acgatctatg taaatgatat gagcaaggct
      841 agtgatgagc agagggagcg tctggtcaga gagcaacgtc actggctaaa gttcagtcgc
      901 aatagctgcc gcgacgagac gtgcttcaag catgcatatt ggtcaaggct ctcggagcta
      961 gaaactttct tcaatcgcga atcgctgccg aagagtgagg cggagagagc cgagacgttc
     1021 aagcagacaa ttgcttcctc gcctttgttt ctcaccgatt ggtctagccc gcaagcattc
     1081 tgcaacaaga tctttggtga tcttaagcag atgaagggcg tcacctttct tgagccggtg
     1141 gcgcgggcgc agagctatga ggacgaggcg ctcgacccct ggaagcggga atgccacgga
     1201 aaggctccac tccacttcag ctaccgattc tgcggaagga atatttcacc ggatgacaac
     1261 aaacggcagg gcggcttgaa gaactgcacc gccggatacg gattaccgcc gtttaagata
     1321 ttcgaactcc cgcccttgac gggttcgacc gacaagcgct acgtgtttta ttcagaatct
     1381 gattacggcc cgatgaacgg cggcaacgcg aagctaaatg gcggaggggc ttcgggctat
     1441 caacaaatcg acattaagga atgccgagcg caggtggggt attcggtgcc aacgtcacga
     1501 agcgagcccg acggaacaaa cgagagcgcg attattgaat atgagggtcg gtactacata
     1561 ctaacagtgg cacgcagttt caatgcgtat tgggtaacgg tgaacacggg ttcccccatg
     1621 tcgggtcaat gcgtttggtc acccgtaaag ccgaaacccc aacagagtca atgaaccgca
     1681 tcgcggttct cacctgagtt tacttctgag cggcagctct gaagatctcc gaaggtccgt
     1741 tcagggtcga tagctccgtg gaacggctgt tattggagtg ccgctcgtga ccggcagcgg
     1801 ccttggctat ctcaccgctg aatcaccctg tccgaggtgc tccgccggca ccgagattcg
     1861 aacttgattg actgcgcaac ggaaaggccc cacttggcgc gcttgaggat cctgtccagc
     1921 ataaaatgtc agcaggtgat ttgacgggaa ggagcatttg agaatggcgt cgataggatt
     1981 gaaagtcagg gatgtcatga gccgcgatgt tcatactgta aaacggaacg acgagctggc
     2041 gatcgccgac gcgttgatga aacaggagcg ggtgcgccac ctcccggtac tcggcgaaga
     2101 cggcgaggtg tgtgctgtag taagccagcg cgacctattc cgcggcgcgc tgctccgggc
     2161 actcggctac ggggcgcggg cggaggagtt aatgctcaag caagtgccag tcaaggacgc
     2221 gatgtcgtcg gaaatccata cgacagcacc ggacacgccg gtcgcagatg cggcgcgcgt
     2281 gatgatcgaa cggaggatcg gatgcctgcc ggttgtcgag aacggcaggt tagttggtat
     2341 tgttacggaa actgacttcg taaggttggt cgccgaaggg cgttagcgtg ccccacctca
     2401 cagacaatca ggtgcggtaa gctgcgacct ctagttacgg cttaccattg gaagtcgata
     2461 tattcgcagc cgagcgaatg tgccttcgtt ctccaatcgt agaggtattc gcccagctcc
     2521 tcctgtttac acgatttccc ttccaaggtg gtgccttcgg gatttgtcac agtggccctt
     2581 accacatact tccagaaaac aaagttccct cttagttctg gttcgaatcg tgcaagctga
     2641 acaattccac atggtccttg tggcgacgac tcagttaccc aaactcccac ccccgagtcg
     2701 cgcatgtatc gaaaacggtc tatgaatgta cgagcactta ctatgcagct gcgcgaatcg
     2761 ttttcatatt caagccgaac caggttgagc caattgtctt ccgttttgga tttgcagaac
     2821 tccacattag ctttttccca gttaagaaga tcctttttct ccgactcggc cgtctccctc
     2881 agcttcttct gcgcttcggc gaccgccttc gagtcatagc ctctaggtgg tgtcttccgg
     2941 ccttcaagaa tatcgacaaa gtccgtgaac cattcgcagt actcggaaga ccactctacc
     3001 ccgtcgctac gaaaggcttc ccgcgcctga ttgagcctag tgctcaagtc ttctggcttt
     3061 gccttcttac tgacaatgat cagattaagt tcgcagtcga ggaacttgtc tcgtttctgc
     3121 tggcacccat agaagatgga agcagcttct ctcgtgctgt ccaagcgccc gtatgtcggt
     3181 gcctcatcgg cccaaccatt cactatccca accagccaga ttcctaccgc cagaagcagt
     3241 gggctacgca acccagaaga gtccgtggcg agcattgctg gaccggctac tgggaaagaa
     3301 cccaatcaac aagctggcgc acttccgcgt cgctcaggct ggcctgcggc cacatgacga
     3361 agcgctcgcc ccaatggcca gcgctgccgc cgcggatgac gtaaggcagc cgttcccgat
     3421 actcgggatt gcgtcggtag cgctgcgcga ttgcactgaa cgagggccca atgtacgaat
     3481 atcccagcgc gtgacattcc aggcagccct tggcgtcagc aagcttatag ccgcggtcga
     3541 aatcttcgcc agggcctgcc acggcgagtg ccggaacggt cgaagcaatc agtatccaaa
     3601 caaacggcct caatctttac cctcctgtct gtcaggaatc tgcgccagct cgtgaactac
     3661 cgctatgggt cgattcgcgg agtagagcgt cgactatttg cgggcttgct ctcggccaat
     3721 agcgtcgaac cacccgtgct ggcctcgggt ccttcgcagc cctgctccgg gcatggcatt
     3781 ttgtagaaga gctcgttcag ataagcagcc acatcacgga tttctacgga gctccagtcc
     3841 tgtcccgcat tcttctccat gcgggtcacc tgatacagca gatcggccca cgatcgcacg
     3901 atgtgcttgt cccgccaatg cgcctgtgtc gtatggcaag cgatacaatg ggtctcgtag
     3961 agtaaacggc cgcgtggcac gtccgcggcg gccaggactt cattctcttg taggcttccg
     4021 ccaacaacgt aggctggtcc gacgccaagc cccataagca acactaagcc aattacgacc
     4081 gcgagctttc gccaagacac gaacacgagc ttggaatagg agttggggat catgatttga
     4141 agaacttgga atcgagggaa taacgagttg ttgggcaaac acccatgtca agagggatga
     4201 tacgtgggaa cacgacgctg cgctgaagcc tagtctgatt cggccagctt cacccagcga
     4261 ggaacgcgaa atgaaagaac gaacgggtgg cgagctaatc caggacttct tgcagacctt
     4321 cgagctgccg tacgtgttcg gcaatccggg gaccaccgag acaacgttcc tggccgccat
     4381 atcggcgtcg aaggcaacgt atgtcctggc gctgcacgaa tcgagtgccg tcggaattgc
     4441 tgccggctac gcactcatga ccggcaagcc atcggtggtg agcctgcata cctatccggg
     4501 cttgccgaat ggcatggttc aatatgcgca atgccctcat agccagagta ccgctgctgg
     4561 tggtcaatgg tcagcaggat tcgcgcttcc tcattcacaa ccccgtcctg ggtgccccga
     4621 acacacggct ggcagagacc gcgacaaagt attcttacga agtcaccagc gtcgttcttg
     4681 tgatcgatcg cgagccgtag ccgattcgga cgcttccgcg gctttggcac gttcgcgcat
     4741 tccgtggaag caagattcag taggccattc gggaacgact cgaaaaggcc accgtccgct
     4801 tggtcggatt cgggcgtccg ctttccggca gcctcaatgt ctcaaacggt cgactgcaga
     4861 agttcacttg gccacgctag ggtgcccgat agcagacctt cgcggacctc ggctatcggc
     4921 cccgatcaga gaagtccggg aacgtccgct tcttgaattt ttggaagtgg cggcgcgagc
     4981 atcgctaaga ccgtttatgt cgcggaaatc gaactagacc tcgtactgca aaaagggagg
     5041 aggctatgtt ctgggaagca atcatccgtg ggctcggcgt actgatccac tgggaaactt
     5101 acatcgcggt agtcctgtac tttgtgatct tcgtcggccc gcttgttctc gtcggccttg
     5161 cggttcagag atcagaggtc ggaaccgggc tcggcggctg cctgatgacg tttgtcatgc
     5221 ccgtgctgca aacgttcgcc attttcgtct tcgtgttaac cctatttccc atcattcttg
     5281 gcgtaggtaa tggcgcagcc tggactcttc catgggttct tggaggtcaa gacccatggc
     5341 tcatggcaag gatggtcgtt gtccttttca tcgctgcgct gctaatggcc ttcgttccac
     5401 tggttggggc ctgggagtca ctgcatacag ctgtgctcgg atcgatcgcg ctaacttttg
     5461 ttattcggat gctagacagc cctgggagtg tgtacagcca tatcgctctt tggcccggct
     5521 tgtggttttt cttgggtctc gttgctgtgg gaggcctgat ggcctgggtc gggaggttgg
     5581 tcgccgttgg tcttggcgta ctagtaagtg ctggtctagc acgtgtactt aagaattcta
     5641 cggaaggaat cgctaccttg ttgatgtttc ctatggcagg catattcggc ttcctcccta
     5701 tgttcctgta cggcgcctac ctcgcagccc agattcattc gagtggttaa ccacgcttca
     5761 attcaaaaca cgtactggat tgggacgagc cagcatcaag tggtcagtga cgggagttgg
     5821 ccgaaccgag acgactaagc taagacacct tggatcacca tgtgccgatc gctcactggg
     5881 gtgacttgga gcgggagccc cgatcggttt acttgcttct tcacctcctc aggagtaaac
     5941 gcagcgcaca gcgaggcgta gaaatcttcc ttgaggatcg gcagctcatt gggtgcgacc
     6001 gtttcgacga tattgcgcgc cgcctgcggt gagtctgggc gcataaggtc cattacgtag
     6061 atcgcagccc cggggcgccc taagcgcttt gcttctctcc acagaaccat cgggtcgggc
     6121 aaatgatgca agagatcctt agaaagaatc gcgtcgaagc tgtgctccgg cagcggtaga
     6181 cccgggatgt agccctgtaa gggcgtgatt cgctcctgga atccggcgct ttgtaccgct
     6241 ttgcgcacga gctcgatcat cgcgtgggag ccgtcgatcg cggtaatacg cgtagtcagt
     6301 cgtgcagctg caaggcgtag catgacatcg ccggggccgc agccaatgtc gaggacgctg
     6361 cctagcctat ctgggtagtc ggcgagcagg tgatcgacat accattggtt ggagatcgag
     6421 aaatcggcct tagcataggc gagtgcctgt tgttcgtcgc ccatgatctc gggttccagg
     6481 attctttcca tgtgaacctc catgaatagc tgactgcaat catacgttca gcatgagcgt
     6541 cggctatagg gaagtttcca cgggatgcgt gaacggccgg tagtggccta ttctgttgaa
     6601 aaactcgtcg aactctcgct ttacacggat gtcataagtc tgggccaaca acgaggggat
     6661 cgagatgatg gggcaacaga gccaggttca acaccagctg ttctacgcct ttaatctcga
     6721 tagtctcgtt ccacccgatc atcttcttcg ccgcattgat cgcttcctag accttggcgg
     6781 cctgcgtcgc catctcgccc gcttctacag ccatacaggc cgcccctcag tggatccgga
     6841 gcttctaatc cgcatgctca tcgtgggcta ctgcttcggt attcgatccg agagacggct
     6901 ttgcgaagag gtacagctca atcttgccta tcgctggttc tgccggctca atttggagga
     6961 tgccgtaccg gagcattcaa cgttctgcaa gaatcgccac ggccgcttcc gagccagcgg
     7021 ggccttccgg cggctgttcg acgaagtcgt gcgcagccgc atggctgccg gagtggtgaa
     7081 gggcgaaggc ttcgccgtgg atgccagtgt ggtagcggcc gatgccagcg gtcaacgcgg
     7141 agtgcccggc aacgagccgg tcaactggaa ggaccccgag ctcagcaccc gggccgtgcg
     7201 cgagtatctg gaagcattgg acaatgagtc gctggctcag acgctgccca agaacatctc
     7261 gctgaccgat ccccagtcgc gctggaccgc cgccccaggc ggcccggcat tctacgctta
     7321 ctcgactaat tacctggtcg acaccgagca tggggtgatc gtggacgtgg aggccacccc
     7381 tgccaatcgc acagctgagg tagagtcggc caagacgatg gtcgatcggg tcgaggaacg
     7441 gtttaagctt gtgcccgagc gcctcatcgg cgataccgct tacgggaccg cttcaatgct
     7501 ggggtggatg gtcgaggaca agggtatcga gcctcatgtg ccgctgtggg acaagacgca
     7561 gcgcaacgac aacactctgt ccagtagcga gttccaatgg aacgaacagg cttacgaata
     7621 ccgctgccca cagggacacg ccttgcgcag ccagcggcgt gccttcaaga aacctcgcac
     7681 acacgtcacc aaggccaaca cgatcatcta tcggtccagt caggccgatt gtgcaacctg
     7741 cccgatgaaa gagcgctgct gtccaaatac atcggttcgc aagattgcgc gtagcatcta
     7801 tgaatcggcc cgtgacgtgg cgcggcgagt gcgaaccact gtgcaatatc ggcgctcacg
     7861 ctacctacgc aagaaggtcg agatcctgtt cgcgcacctc aaacgaatcc tcaaactgga
     7921 tcgcctacgc ctacgaggcc taagcggtgc cagagatgaa ttcacgctgg ctgctgctgt
     7981 tcaaaatcta cggcgtctcg ctacgctgat tccgcaagga ccaccagcac ccgcttgagg
     8041 cgaaaagcaa ccatcgaaat ccagcctggg gcccagaacc tagttgaatc gcccccgact
     8101 ggggacgaag cgctggacct gattctctct tggacgccgc cgccggccac cggagatccc
     8161 cgctccgtag ctgagttatt caacggaata ggccacgagc agtccttcga actcgtcgtc
     8221 cgattcgtgc gattgcagac gtcagccgct tctaacgtgg ttgtgaaccg cgcgccatga
     8281 tgagctacgc aagcccgcat ccgcgcctcg gcagaaaaca ctcgccgctt cgattatcga
     8341 agaatcagaa tagcgccgac aacgaaaacc ctaaggcaag taaccagccg ccagctgccg
     8401 cgaacgcgac cacgatgacg ggggagactt tccagaacat gagcagcgtg aatgcggcga
     8461 gcgccaagcc gaagtcggcg cgcgatttga ccgcgctggt ccaaaccgga tcgtacagcg
     8521 ctgccaacag caggccgacc acggcggcgt tgatgcccct caacgct
//
LOCUS       DASPUL010000129         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_571333, whole genome shotgun sequence.
ACCESSION   DASPUL010000129 DASPUL010000000
VERSION     DASPUL010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571333"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VEJ00_03255"
     CDS             <1..807
                     /locus_tag="VEJ00_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HXX00206.1"
                     /translation="SIGVLIVLFLLASFFGYLTALHPSFSAAGFTAALILVFGGTGEP
                     WHTAWLRVLYTVEGAFIAFAVGALIWPVHAREGLRQKLAKILEGTGTLYRAVTDAALS
                     GIDNEQEVRELDRRLHDLRRGITQQMDEARSELSFSRFNQSAYQRVIDLEDQVRRRLT
                     AMAEDSSLYVHAQVDLGVVPSLPRLAEKTAKNFTLLAEAARHPGRPLDSCGLDDAIRD
                     LDFDLAKLRAQRATAPFALDRMLPFWALVFNLREIAQDLKQLESALPQLA"
     gene            1104..2006
                     /locus_tag="VEJ00_03260"
     CDS             1104..2006
                     /locus_tag="VEJ00_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00207.1"
                     /translation="MKWRALGTAVVVVLLSALALSQAADQYLDVYIAQVKPEKRADFD
                     AIGKKMVAANRQNHGDNWITMETAYGPGNRITFVSTRNSYAETETGGDAFNAAVQKAF
                     GKAGTDKLFQDYSQCIASYRNELRRRRWDLSSNAPADAAAEAKMVGNTRWLRTTAVHV
                     RAGRADEFEALLKDVKAAREKASPSVATLVSQAVAGQEGTVYYITTLENSMAGFDSIP
                     SLQKALGDEGYAKFLKTSADVVERVETAINHFLPELSNPPEQVMAAAPDYWNPKPMVA
                     AKGKTKTGEMVNASEKGKTEEKNK"
     gene            complement(2209..2589)
                     /locus_tag="VEJ00_03265"
     CDS             complement(2209..2589)
                     /locus_tag="VEJ00_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00208.1"
                     /translation="MRYLVRARVRRGREGDLLDAIEQETLGRGSVAEGEYLRNMQEAR
                     WCADQTARWVEVCYCPTPLLEERPYWEEYFDLTRVQDAHDRGKCRDENGSEPWACGDC
                     DCTARLEEKLKQSGKPFLDVLRGK"
BASE COUNT          578 a          871 c          799 g          531 t
ORIGIN      
        1 tccataggcg tcctgatagt gctcttcttg ctggccagct tcttcggata cctgaccgcc
       61 ctgcatccca gcttcagcgc cgccggattc actgcagccc tcatcctggt ctttggcgga
      121 acaggagagc cctggcacac agcctggctt cgcgtgcttt acacggtcga aggcgcgttc
      181 atcgctttcg ccgtaggcgc gctgatctgg ccggtgcacg cacgcgaagg cctccgccag
      241 aagttggcca aaatcctgga gggaaccggc accctctacc gcgccgtcac cgatgccgcg
      301 ctctcgggca tcgacaacga gcaggaagtc cgcgaactcg accgccggct gcacgacctc
      361 cgccgtggga ttacgcagca aatggacgaa gcccgcagcg agctgtcctt ctcccgcttc
      421 aaccagagcg cttatcagcg agtcatcgat ctggaagatc aggttcgccg ccgcctcacc
      481 gccatggccg aagacagctc gctgtacgta cacgcgcagg tcgatctggg tgtggtgccc
      541 tcgttaccgc gactggcgga gaaaaccgcg aagaacttca ccctgctggc ggaggcagct
      601 cggcatcccg gcaggccact ggattcatgc ggactcgacg atgccattcg cgaccttgat
      661 ttcgatctgg ccaaactccg cgcccagcgc gcgacggcgc ccttcgctct cgatcgcatg
      721 ctgcctttct gggcgctggt gttcaacttg cgggagattg cgcaggactt gaagcagttg
      781 gaatcggccc tgccgcagct ggcctgagcg gcgagattct ctctgcggcc tcggcggcag
      841 ttctcggtag tctgcgtgct gagagtttca gatggctgag cacgcagaaa acgcgcagag
      901 atcgcggaaa gagtcctcgc gaaaggcaac gcttagtcgg cgcgcattat ccccaacagg
      961 cagagccgtg cccattcggg accggtttcc tggcctagcc caaaagcccg cccgttgcac
     1021 ccttgacggc gctcgcgcac gggagtacgt taggcccgct cgggaaaaac aacataacaa
     1081 tccatcctga aaaggaggct tctatgaagt ggagggcctt agggaccgca gttgtcgtgg
     1141 tgttgttgtc ggcgctggcg cttagtcagg ctgctgacca atatctcgat gtctatattg
     1201 cacaggtcaa accggaaaaa cgtgcagact ttgacgccat cggcaagaag atggtggccg
     1261 caaaccggca gaaccacggt gacaactgga tcacgatgga aaccgcctat ggtccaggga
     1321 atcggatcac attcgtcagc acgcgcaaca gctatgccga aaccgaaacg ggtggggacg
     1381 ccttcaatgc cgctgtccaa aaggctttcg gcaaagccgg tacggacaag ttgttccagg
     1441 actacagcca atgcatcgcg agttaccgca acgaactgcg ccgccgccgc tgggacctga
     1501 gcagcaatgc tccggcggac gccgcggccg aggctaaaat ggtcggcaac acgcgctggc
     1561 tgcgcaccac cgccgtgcac gttcgcgcgg gtcgtgccga tgaatttgag gcgctgttga
     1621 aggatgtgaa agcagcccgc gagaaggcat caccgtcggt cgccacgctc gtgtctcagg
     1681 ccgttgctgg ccaggaaggc acggtgtact acatcacgac gctcgagaac tccatggcgg
     1741 gattcgattc gattccatcc ctgcaaaaag ctctcgggga cgaaggctac gcgaagtttc
     1801 tcaagaccag cgcagatgtg gtcgagcgcg tcgaaaccgc tatcaaccac ttcctgcctg
     1861 aactgagcaa tccgccggag caggtgatgg cggccgcacc ggattactgg aatccgaagc
     1921 ccatggtagc cgcgaaaggc aaaaccaaaa ccggcgagat ggtcaatgcc tcggagaagg
     1981 gcaagaccga ggagaaaaac aagtagtcag aaacgagtat tccctcccca gaccagggag
     2041 cgtcgccggc cggttcagta ttcaagatcg gccggcggtt tttcttctgc aagcttttat
     2101 tccggggtcg tgaaagccgt ttgcctgctg ttctcagcgt cctttgcgga cgttccttgc
     2161 gaactttgcg gtaaagggtt ttcgttcctg cgaaaaggac aagcgaggtt acttccctct
     2221 gaggacgtcg aggaacggct tgcccgactg cttcaacttc tcttccagcc ttgccgtgca
     2281 atcgcaatcg ccgcaagccc agggctccga gccgttctcg tcgcggcatt tgccgcggtc
     2341 gtgcgcgtcc tggacgcgag tcagatcaaa gtattcttcc cagtacggcc gctcttccag
     2401 caatggcgta ggacagtaac aaacctccac ccagcgcgcg gtctggtcgg cgcaccaccg
     2461 ggcttcctgc atgttgcgca ggtattcgcc ctcagcgacg gagcctcgcc ccagagtctc
     2521 ctgctcgatg gcgtctaaca gatcaccttc ccgcccgcgt ctcactcgcg cccgcaccag
     2581 gtatcgcatg aggtcccggc acaaacaaaa gcattgttga tccttgattg acgattgtta
     2641 aatcaagcca cccgatcagc cctcattcaa caatcaaaag tcaacaatca acaatgcttt
     2701 tcagatgaac atctcgtcct gcggcaccgg acgccgttcc tcacgccggt cgccgttctt
     2761 gcggccgcgg ccgccgaac
//
LOCUS       DASPUL010000130         2504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_430547, whole genome shotgun sequence.
ACCESSION   DASPUL010000130 DASPUL010000000
VERSION     DASPUL010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430547"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /gene="lhgO"
                     /locus_tag="VEJ00_03270"
     CDS             <1..990
                     /gene="lhgO"
                     /locus_tag="VEJ00_03270"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HXX00209.1"
                     /translation="AMVEFCREHGIPHKICGKVIVATRPEEVPRLEDLRQRGEANGLT
                     GLRVIGPEELREIEPHAVGLQGLVVPSTGITDYAAVCEKYAELISAAGGSVVTSAAAT
                     AIRRSNDEIVVETSRGAFPTNALINCAGLFSDRVARMAGDNPRVMIVPFRGEYYDLTA
                     ERASLVRALIYPVPDPQFPFLGVHFTRRITGKVDAGPNAVLALAREGYRHTDINPRDL
                     VSSLTFPGFWRMARRHWRNGLGEWHRSLSKPAFVRALQTMLPEVTASDLVPGGSGVRA
                     QALKPDGALVDDFQFVPSGNILHVLNVPSPAATASLTIAKTIVDSAAAGLGLS"
     gene            complement(992..>2504)
                     /locus_tag="VEJ00_03275"
     CDS             complement(992..>2504)
                     /locus_tag="VEJ00_03275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXX00210.1"
                     /translation="SETNARWSPDGSTLAISYRPETSSTTNIALLDWKTRKVRKLTDE
                     QMRNREWGEALWSADGKTIYADRANAGHTDSDVYRIEVATGKLENLTPHQGDVLYSVS
                     SVSPDGQTLLITSNEKGGYDNVALLDVASKKRTWVTDLKWEASGGHFAPDGKSFTYTL
                     NADGRTDIYVVDRVSGRAVRIDFPVGISFPAGDPTAFSPSGDRLLVSHQSSTEPADIW
                     VYDMNAHMPRQLTFSALASLNPARLPASQLVHYKTFDGKTISAFLWMPFNLKRNGSNP
                     GIVLPHGGPTGQTVDSFSKTAAALASRGYVCIAPNVRGSTGYGMEFQRSNYQDLGGGD
                     LQDEVFAAHFLVDTGYVNARKIGITGGSYGGYMAMIAIGRTPDDWAAAVELFGITDWL
                     TEQEHEEPTLQQYDQSILGDPVKDRKAYEDASPIKYFKNAKAPLLILQGTNDIRDPKE
                     EAEQAETILKKEGKIVDSHYYADEGHGFAKRENQIDAMRRTVEWFDKYLKNKP"
BASE COUNT          445 a          845 c          669 g          545 t
ORIGIN      
        1 gccatggtcg agttttgccg cgagcacggc atcccgcaca agatttgcgg aaaagtgatt
       61 gtggccacgc gtcccgagga agtcccgcgc ctcgaagatc tgcgccagcg cggcgaagcc
      121 aacggactta cgggcctgcg cgtcatcggc ccggaagaac tccgcgagat cgagccgcac
      181 gctgtcggcc tgcagggcct ggtcgttcca tccaccggaa tcaccgatta cgccgcggtc
      241 tgcgaaaaat atgccgaact gatttccgcc gcgggcggca gcgtcgtcac ttctgcggct
      301 gccaccgcta tccgccgctc caacgatgag atcgtggtcg agacctcccg gggtgcattc
      361 ccgacgaatg ctctcatcaa ctgtgccggc ttgttcagcg accgcgttgc gcgtatggcc
      421 ggcgacaatc cccgagtcat gatcgtgccc ttccgcggcg aatactacga tctgacggca
      481 gagcgtgcct cgctggtccg cgctctgatc taccccgtcc ctgatcctca atttcccttc
      541 ctgggcgtgc atttcacgcg ccgcatcacc ggcaaagtcg acgccggtcc caacgccgtc
      601 cttgctctcg cccgtgaagg gtaccgtcac accgacatca atccgcgcga tctcgtttcg
      661 tcgctcacat tcccaggttt ctggcgcatg gcccgccgtc actggcgcaa cggcctcggt
      721 gagtggcatc gctcgctctc gaagcctgcc ttcgtgcgcg ccctccagac catgcttccg
      781 gaagtgaccg caagcgatct ggttcctggc ggttccggcg tccgtgccca ggccctcaaa
      841 cccgatggcg cgctcgtcga tgactttcag ttcgttccct cggggaatat cctccacgtg
      901 ctcaacgtgc cttcaccggc cgcgaccgcg tcgctgacca tagcgaaaac gatcgtggac
      961 tccgccgccg ctggtctggg attgtcctag ctcacggttt gttcttcagg tacttgtcaa
     1021 accactccac cgtccgccgc atcgcgtcga tctgattctc cctcttggca aagccgtggc
     1081 cttcatccgc ataatagtgg gagtccacga tcttgccttc ctttttcagg atcgtctccg
     1141 cctgctccgc ctcttccttc ggatcacgaa tgtcattcgt tccctgcagg atcagcagcg
     1201 gcgccttcgc gttcttgaag tacttgatcg gcgacgcatc ttcataggcc ttgcggtctt
     1261 tgaccgggtc acccaggatt gactggtcat attgctgcag cgtcggctct tcgtgctcct
     1321 gttcggtgag ccagtcggtg attccgaaca attccaccgc cgccgcccaa tcatccggcg
     1381 ttcttccaat ggcgatcatc gccatgtatc cgccgtacga gccgccggta attccaatct
     1441 tgcgcgcatt cacgtagccg gtgtcgacca gaaaatgcgc ggcaaacact tcgtcctgca
     1501 gatccccgcc tcccaggtcc tgataattcg aacgctgaaa ttccattccg tagccggtcg
     1561 aaccgcgaac attcggtgcg atgcagacgt agccccgcga agccagggcg gctgccgtct
     1621 tgctgaaact gtccactgtc tgccccgtcg gtccgccatg cggcaacacg atccccggat
     1681 tgctcccgtt ccgcttcaga ttaaacggca tccacagaaa cgcgctgatc gtctttccgt
     1741 cgaaggtctt gtaatgcacc agttgcgaag ccggcaaccg cgccggattc aaactggcca
     1801 aggccgaaaa cgtgagttgt cgtggcatgt gagcattcat gtcgtacacc cagatgtccg
     1861 ccggctcggt cgaactctgg tgcgaaacca gcagccggtc gccgctcggt gaaaaagccg
     1921 taggatcgcc agcagggaag ctgatgccca cagggaaatc gattcttacc gcgcggcccg
     1981 aaacccgatc gaccacataa atatccgtgc gcccatcggc attcaacgtg taggtaaagc
     2041 tcttgccgtc gggagcaaag tgcccgccgc tcgcttccca cttcaagtcc gtgacccagg
     2101 ttcgtttctt gctggcgaca tcgagcaagg caacgttgtc gtatccgcct ttttcattcg
     2161 aagtaatgag cagcgtctgc ccgtcgggcg aaaccgacga cacgctgtag agcacatcgc
     2221 cctggtgcgg cgtaagattc tcgagctttc ccgtagccac ctcgatgcgg tacacgtccg
     2281 aatccgtgtg ccccgcattt gcccggtccg cataaatcgt ctttccgtcc gcgctccaga
     2341 gtgcttctcc ccactcccgg ttacgcatct gctcatcggt cagcttccgc accttccgcg
     2401 tcttccagtc aagcagtgca atgttggtag tcgaggaagt ctccggccga taggaaattg
     2461 ccagggtgct tccatccggc gaccagcgcg cgttcgtttc cgat
//
LOCUS       DASPUL010000131         6508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1937322, whole genome shotgun sequence.
ACCESSION   DASPUL010000131 DASPUL010000000
VERSION     DASPUL010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937322"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VEJ00_03280"
     CDS             complement(<1..227)
                     /locus_tag="VEJ00_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00211.1"
                     /translation="MAEPERASSPAPSSEAPLPKVEASTAPAPTLVASLEAHPAVAGH
                     HPEARSHLRVPWLAALGATCGYFIGIGTYGE"
     gene            553..2661
                     /gene="fusA"
                     /locus_tag="VEJ00_03285"
     CDS             553..2661
                     /gene="fusA"
                     /locus_tag="VEJ00_03285"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXX00212.1"
                     /translation="MKVYSGENIRNVALVGHGHAGKTSLVSAMLYTSGATPRMGRVDD
                     GSTTTDYDDEEIARQMSITTGVAVVEWGKTKINLLDTPGFNMFVHEAKMALPVVDAAI
                     VVVDGVAGVEVVTQRVWNYCEEYKAPRLIVVNRMDRERADAARVLESVEKAFGRNVIP
                     IELPIGAEKSLSGVIDLVRMKAYTYELGGSGRGKEAEIPANLKEQAQEAHERLVELVA
                     EGDDKLMEKFFEAGTLGEEDLVPALHNAIREDKIFPVIFSSGLGNIGADRVMDFIVDY
                     TPAPSEHEWVQGEAAASGNGDAPKRHETDAEPVSLYVFKTVSDPFAGRISYFKVFSGV
                     AKNESTAQNFRTNSSEKLSHISVMQGKTAIPITELHAGDIGAVAKLKDTLTGDTLGDK
                     SAPIQYPRVKLPEPAITFAIEPKSRADEDKLGPGPHKLMEEDAMLRFFRDPQTKEFLI
                     AGTGQQHIEVTVSKLKKRYNTQVNLKAPKVPYRETIRGKADVQGRHKKQTGGHGQFGD
                     CKIKMEPLPRGGEFEFVNDIFGGAIPKNFIPAVEKGIKDAAARGYLAGYPVVDFKVVL
                     YDGSYHDVDSNDLSFQLAGRIAFKKAMEVAKPTLLEPIMNVEITVPDDFAGSIMGDLN
                     SRRGRIQGMDNKAGNTIVKAEVPMAEMLTYGVELTSMTQGRGSFNMEMHHYDIVPGQL
                     QEKIIEKAKSERGEQPAEEE"
     gene            complement(2846..3235)
                     /locus_tag="VEJ00_03290"
     CDS             complement(2846..3235)
                     /locus_tag="VEJ00_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXX00213.1"
                     /translation="MVIDTSAIAAIFFIEPERDTFLEAIASAGNRLMSAAAVLETGIV
                     LESRQGEAAGREFDLFAVRAGLEIVPVDAEQAEIARSAWRRYGKGRHAAALNFGDCFS
                     YALAKSTGEPLLSKGTDFALTDIQVWK"
     gene            3259..3381
                     /locus_tag="VEJ00_03295"
     CDS             3259..3381
                     /locus_tag="VEJ00_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00214.1"
                     /translation="MLVIPHDFLGAAGVEDWQRVHALKYVSQLAQQEVTFALFL"
     gene            3557..4609
                     /gene="ruvB"
                     /locus_tag="VEJ00_03300"
     CDS             3557..4609
                     /gene="ruvB"
                     /locus_tag="VEJ00_03300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HXX00215.1"
                     /translation="MATARNNPNLNYNRERLVSAAAVEDDSSFELKLRPQKLSEFIGQ
                     KKVKENLAVAIEAARSRGEAMDHVLLYGPPGLGKTTLANIIANELGVHFQPTAAPLLQ
                     IKGDLTAIITNLRDRQVLFIDEIHRLQPVLEELLYSALEDYKLDIIIGQGPSARTHTL
                     EVKPFTFVGATTRAGLISSPLRARFGIVLRLEFYTPEDLRVIVERSAEILGVEIDDAG
                     AREIASRSRGTPRIANRLLRRVRDYAQVRGAGKIDEPTAKAALQMLEVDQYGFDEVDR
                     RLLLTIIEKYQGGPVGLNTLAAVLAEEPDAIEEIYEPFLMQIGFLDRTQRGRVATQLA
                     YEHFGITPSRRQSTLF"
     gene            4971..>6508
                     /locus_tag="VEJ00_03305"
     CDS             4971..>6508
                     /locus_tag="VEJ00_03305"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HXX00216.1"
                     /translation="MQTSQAAPQLGPPALTIPRLQRPPQLADFLDMRPEGEIAQQMAR
                     VTGFTQRNPHDGENVSELTEAYLGYDQKNLYVVFVCFDDPKQVRARLSAREDVSDDDQ
                     VEIILDTFHDRRRAYMFQTTPLGVQWDAIWTEASREEETEGHFDTSFDTVWDSRGKVT
                     DRGFVVWMAIPFKSLRFPSTREQAWGIILYRGITRKTEDSFWPHVSYKVEGRLGQGAT
                     LYGLEGISPGRDIELIPYGVARGFRALDTRGAPFVQQARVQGQPGMDAKFVIHDHFTV
                     DMAANPDFSQVESEDPQITVNQRFAVYFPEKRPFFLENEDFFRTPMNLFFTRNIGDPS
                     AGIRLTGKDGPYSVGLMAADDRSPGLAVPNFSPLFGTRSYFTIARVSRDIFHQSSVGA
                     FYSDWECPTTGEFNRLGGLDTRLKFNANWTLDGQAVVSSSDLNLNCEANLNPFNSGNR
                     GNNNYYAGPADNLELHREGLHFTYDGTYNDITPGFVTVPGFINRVDIRETVQTADYRF
                     RPKSG"
BASE COUNT         1351 a         1844 c         2117 g         1196 t
ORIGIN      
        1 aactcgccgt atgtgccgat gccaatgaag tatccgcagg tggcgcccag ggcggcgagc
       61 cagggcactc tcagatggct tctggcttct ggatggtgcc ccgcgactgc agggtgtgct
      121 tcgagggaag cgaccagggt cggcgccggc gccgtcgaag cttcgacttt cggcagcggt
      181 gcttccgagc ttggggctgg tgaagaagcg cgttccggtt cggccataac atctcccgga
      241 gaagtacaga tggggcaata tcctacacca gagagccagc ggtgggggat ggcacgagag
      301 ggggaagaag cacacatttc aggaccgagg gtcaggcgcc ggaatactct gtgtccactc
      361 cagctgtggt gcgcaccctc gagcttcgct cgaccggaca gccgggggcg gctgtcccta
      421 cgcgatcggt tgagacgccc gcttcccgcg tggtagcatc ctaggttttg gcagcgatgt
      481 cgggcgggcc tgtattgtcc taatcctctt ccataggccg tttctttcct gcgcctgccg
      541 aaggagccca acttgaaagt ctattcggga gaaaacatca ggaatgtcgc attggtgggg
      601 cacgggcatg cggggaagac ctcgctggta tcggccatgc tctatacgtc gggggcgacg
      661 ccgcgcatgg ggcgcgtgga tgacggctcg acgacgaccg actacgacga cgaggaaatc
      721 gcgcgacaga tgtcgattac gaccggcgtg gccgtggtgg agtggggaaa gacgaagatc
      781 aatctgctcg acacgcccgg cttcaacatg tttgtgcatg aggccaagat ggcgctgccc
      841 gtggtggacg ccgccattgt ggtagtcgac ggagtggccg gcgtggaagt tgtgacgcag
      901 cgcgtgtgga actactgcga agaatataaa gcaccgcggc tgatcgtggt gaaccgcatg
      961 gatcgcgagc gggccgacgc ggcacgggtg ctggaatcgg tggagaaggc gtttgggcgg
     1021 aacgtgattc cgatcgagtt gccgattggg gcggagaaga gcctttccgg tgtgatcgac
     1081 ctggtgcgga tgaaggctta cacctacgag ttgggcggca gcggaagagg gaaagaggct
     1141 gagattccgg cgaatctgaa agagcaggcg caggaagcgc acgagcgtct ggtggaactg
     1201 gtggccgagg gcgacgacaa gctgatggag aagttcttcg aggccgggac gctcggagaa
     1261 gaagatctgg ttccggcgct gcacaatgcg atccgcgaag acaagatttt ccccgtgatc
     1321 ttcagctcgg ggctgggaaa cattggcgcc gacagggtga tggatttcat cgtggactac
     1381 acgccggcgc cttccgagca cgagtgggtg cagggcgagg ctgccgcttc cggcaacgga
     1441 gatgctccca agaggcatga gactgacgcg gagccggtgt cgctgtatgt gttcaagaca
     1501 gtgtcggatc cgttcgcagg aaggatctcc tacttcaagg tgttttcggg agtggcgaag
     1561 aacgagtcga ccgcgcagaa tttccgcacc aactcatcgg agaagctgtc gcatatctcc
     1621 gtaatgcagg ggaagacggc gattcccatc acagaactgc acgcgggcga cattggcgcg
     1681 gtggcgaagc tgaaggacac gctcaccggc gacacgctgg gcgacaagtc ggcgccgatc
     1741 cagtatccac gggtcaagct gccggagccg gcgatcacct tcgccatcga gccgaagagc
     1801 cgcgccgacg aagacaagct cggcccaggt ccgcacaagc tcatggaaga agatgccatg
     1861 ctgcgcttct tccgcgatcc acagaccaag gaatttctga tcgcgggcac ggggcagcag
     1921 cacattgaag tcacagtctc gaagctgaag aagcgctaca acacgcaggt gaatctgaag
     1981 gcgccgaagg tgccgtatcg cgagacgatt cgcggcaagg ccgacgtgca ggggcggcac
     2041 aagaaacaga ccggcggcca cggccagttc ggcgactgca agatcaagat ggagccgctg
     2101 ccgcgcggcg gagagttcga attcgtcaac gacattttcg gcggcgcgat cccgaagaat
     2161 ttcattccgg cggtggagaa gggcatcaag gacgcggcgg cgcgcgggta tctggcggga
     2221 tatccggtgg tggacttcaa agtggtgctc tatgacggct cgtatcacga cgtcgactcg
     2281 aacgacctgt catttcagtt ggcggggcgc atcgccttca agaaggcgat ggaagtggcc
     2341 aagcccacgc tgcttgagcc gatcatgaac gtcgagatca ccgtacccga cgacttcgcc
     2401 ggatcaatca tgggcgatct gaacagccgg cgcgggcgca tccagggcat ggacaacaag
     2461 gctggcaaca cgatcgtgaa ggcagaggtg cccatggccg aaatgttgac ctacggtgtg
     2521 gagttgacat cgatgaccca gggccgtggc tcgttcaaca tggagatgca ccactacgac
     2581 atcgtgcccg ggcagctgca ggagaagatc atcgagaagg caaagagcga gcgcggggaa
     2641 cagcccgcag aagaagaata gagatccttc gctgcgctca ggatttcggc agcaggctcc
     2701 cgcttcgctc acgcctgcta atcgcctcaa gctgcaggag aagattatcg agaaggcgaa
     2761 gagcgagcgc ggcgaggtga aggaagagga agagtagctg ctagcgggta ctaatggcga
     2821 ggcggcggcc tgtcgggccg gctcttcact tccacacttg aatgtcggta agagcaaagt
     2881 ccgtgccctt cgacagtaag ggctcaccgg ttgacttcgc caaggcgtac gagaagcaat
     2941 ctccaaagtt gagcgcggct gcatggcggc cctttccgta tctgcgccag gcggagcgcg
     3001 caatctcggc ctgctcggcg tccaccggta cgatctcgag accggcgcga accgcaaata
     3061 agtcaaactc tcttcccgct gcctctccct ggcggctctc aagaacaatt ccggtttcaa
     3121 gcaccgctgc ggcggacatc agacggtttc ccgcggacgc aatcgcctca agaaaggtat
     3181 cgcgctccgg ctcgataaaa aagatggcag cgattgccga ggtgtcgatt accatactgt
     3241 caatcagcga ggaagcccgt gctcgtcata ccccacgatt tcctcggggc tgcgggagtc
     3301 gaggactggc agcgcgtcca cgcgctgaag tatgtctcgc aactggctca acaggaggtc
     3361 acttttgcgc tgtttctgta atcgttccag gcgctcctcc agggaagtct tgattgcgcc
     3421 agtcagggac tcacctgttt tggaagcgac ctcacgagct aaccgctcga cttctatact
     3481 cttgatacta atgcccatac agcatcgatt ataccatact gcaatttagt atatacttat
     3541 tgaacggaac gaacctgtgg ccactgcccg aaacaatccc aacctcaact acaaccgcga
     3601 gcggctggtg tcggcggcgg cggtggagga cgattcttcg tttgagctga agctgcggcc
     3661 gcaaaaactc tcagagttca tcgggcagaa gaaggtcaaa gagaatttgg cggtggcgat
     3721 cgaggcggcg cgctcgcgcg gcgaggccat ggatcacgtg cttttgtatg ggcctccggg
     3781 gctgggtaag acgacgctag ccaacatcat tgccaatgaa ctgggtgtgc actttcagcc
     3841 tacggcggcg ccgctgctgc agatcaaggg cgacctgacg gcgatcatca ccaacctgcg
     3901 ggaccggcag gtgctcttca tcgacgaaat ccatcgcctg cagccggtgc tagaggagtt
     3961 gctctactcg gcgctcgaag actacaagct cgacatcatc attgggcaag gcccgtcagc
     4021 tcgcacgcac acgctcgaag tgaagccgtt cacctttgtg ggcgcgacga cgcgggcggg
     4081 gctgatttcg tcgccgctac gggctcggtt cgggatcgtg ctgcggctgg agttttatac
     4141 gccggaggat ttgcgggtga tcgtggagcg ctcggccgag attctgggag ttgagattga
     4201 cgacgccgga gcgagagaga ttgccagccg gtcacgcggg actccgagga ttgcgaatcg
     4261 cttattgcgg cgggtgcgcg attacgcaca ggtgcgcggt gcgggcaaga ttgacgagcc
     4321 tacggccaag gcggcgctgc agatgctgga agtcgatcag tacggattcg atgaggtcga
     4381 ccggcggctg ctgttgacga tcatcgagaa atatcaggga gggccggtgg ggttgaacac
     4441 gctggccgcg gtgctggccg aagaaccgga cgcgatcgaa gaaatctacg agccgttcct
     4501 gatgcagatc ggatttctcg accgcacgca gcgcgggcgt gtggccacgc agctggcgta
     4561 cgagcatttc gggatcactc cgagccgcag gcagagtacg ctgttctgaa ttggactccc
     4621 acgctcaggg gctaaagccc cctcccttta cccgacctct acgcggcgct gaagcgccgc
     4681 tcttccacgg cggcttcgac cgtcacgaat aactgttcgg ccactactgt gttcggcaaa
     4741 ccgcagacaa gtcttttccg gttgggcccg cgcggctgat ggtgtatctt gctcaatccc
     4801 gctcggacag ggacgggcga gggtctcgga gccggggcgt gatttttctt cttccaggga
     4861 aagggcggct gccatttcga tccgcggggg ctctgctgcc ggccattgcg ctattgtctg
     4921 gctgggccgc gagggcatca gatccttcag ccgcaggcac gaacccccac atgcaaacat
     4981 ctcaggcggc cccccagctt gggcctccgg cgctgaccat cccacggctg cagcgtccgc
     5041 cacagctcgc ggattttctc gacatgcggc cggaaggcga gatcgcgcag cagatggcgc
     5101 gggtgaccgg cttcacgcag cgcaatccgc atgacgggga gaacgtttcc gaacttaccg
     5161 aagcctacct gggctacgac cagaagaatc tctacgtcgt cttcgtctgc ttcgacgatc
     5221 ccaaacaggt gcgcgcccgc ttgtcggcga gggaagacgt gtccgacgat gatcaggtgg
     5281 agatcatcct cgacacgttt cacgaccggc ggcgcgcgta catgttccag acgacgccgc
     5341 tgggtgtgca gtgggacgcg atctggaccg aagcttcgcg cgaggaggaa accgaagggc
     5401 atttcgacac atcgttcgac accgtgtggg actcgcgcgg caaagtgacc gatcgcggct
     5461 tcgtggtgtg gatggcgatc cctttcaaga gcctgcgctt tccgtcgacc agggagcagg
     5521 cgtggggaat catcttgtac cgcggcatca cgcgcaagac cgaagactcg ttctggccgc
     5581 atgtttcata caaggttgag ggccggctgg ggcagggggc gaccctctac gggctggagg
     5641 gaatctcgcc ggggcgcgat atcgagctga ttccttatgg tgtggcgcgc gggtttcgcg
     5701 ccctggatac gcgtggcgcg ccgtttgttc agcaggcccg ggtgcagggc caacccggca
     5761 tggatgcgaa gttcgtgatc cacgatcact tcaccgtgga catggcggcg aatccggatt
     5821 tcagccaggt ggaatcagaa gatccgcaga tcacggtaaa ccagcggttc gccgtctact
     5881 tcccggagaa gcggccattt ttcctcgaga acgaagactt cttccgcacc ccgatgaacc
     5941 tgtttttcac gcgaaacatt ggcgacccgt cggcgggaat acggctaacg ggcaaagacg
     6001 ggccgtactc ggtgggactg atggcagccg acgaccgcag ccccggcctg gcggtgccca
     6061 acttcagccc gctcttcgga acgcgctctt atttcacgat tgcgcgggtg agccgcgaca
     6121 tctttcacca gtcgagtgtg ggagcgttct acagcgactg ggaatgcccg acgacgggcg
     6181 aattcaatcg cctgggcggc ctcgacacac gcctgaaatt caacgccaac tggacgctcg
     6241 acggccaggc ggtggtcagt tcgagcgatc tgaatctgaa ttgcgaagca aacctgaatc
     6301 cgttcaactc cggcaatcgc gggaacaaca actactatgc cgggcctgcc gacaatctcg
     6361 aactgcaccg agagggcctg cacttcacct acgacggaac gtacaacgac atcacgcccg
     6421 ggtttgtcac ggttcccggt ttcatcaatc gcgtcgacat ccgggagact gttcagacgg
     6481 cggactaccg ctttcggcca aagtcagg
//
LOCUS       DASPUL010000132         4711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1937480, whole genome shotgun sequence.
ACCESSION   DASPUL010000132 DASPUL010000000
VERSION     DASPUL010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1937480"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="VEJ00_03310"
     CDS             complement(<1..1103)
                     /locus_tag="VEJ00_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HXX00217.1"
                     /translation="MKRHLCLLLFSGWLLTFSVRSLASDAKVLTNHLGYEAAGPKHAV
                     ILGKATDSFSACALKDEASDQQVLSVPAKAAGPVEKWRDWYFWMLDFDSFSTEGQYYL
                     ECASEAGSVRSFPFLVQRNLLQRNTMQDVIYYFKAERSSGEYDKADSHLRFEAAKKGT
                     WDAHGGWWDATGDYGKHFSHLSFSTYFNPQQIPLVVYSLLKSFEQNKLHGPPELARYQ
                     AHLLDEAIFGADYLVRMKAPGGSFYRSVSNGGVDQVPEKRKIAGEMKRFGIEAAPGQG
                     AKGMVESAENEREYEVSYRSGGGIAIAALAMASMLPVSGQFTSADYLKSAEDAFDDLE
                     KDNLKLVNDGKENIVDDYCALAAATELYRATRR"
     gene            1207..3267
                     /locus_tag="VEJ00_03315"
     CDS             1207..3267
                     /locus_tag="VEJ00_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00218.1"
                     /translation="MPRSGSISPRLLPLFLLACSLSVLAAEQSPWLEVHSAHFTVITD
                     AGEKKGREVALRFEQMRAVFASLLGKERLNQPVPLTILAFKNDKMFYQCAPLRQGQPI
                     DVPGFFLPGEDQDFIVLNLFEEEPWRAVAHDFAHMLLNYNYPPAQGWFDEGLAEYFSS
                     IRVDDKGVEIGGDPELRPSVASDLLQNQIDTHPPKSLTELLGAQVWLSIPDLFTMKHD
                     TSGYNEGTHHTLYYAESWIVMHYLLHQKKLPETGTYFGLVLNQRLPVEEAIQKAYGMS
                     SAELEQAVRDYFHSQSSLLTALDNARQNAGHGENSAQAYRFPAPLGPEDSAITAAPLP
                     ESDARALYAGVQVRIPERREFGLKELQTLATTTTAADIKFAAKAEKAQEKADQDEKQL
                     PSTAVGNALAHRILAWDSIEHGDFEKGLTELGNAAALNRNDMWLRYYLSMLKYRMAQS
                     KHTEIQGLANMMLDLRAVLEWYPEMADAYDLLAVARNEGGTSANAMQAERAAMNLSPR
                     DERYVYHMAQIYISGKQWQAADDLLDRLRGSNNPQMAALARETQSQIANQRKYGMTLG
                     TAAPQTKLAPQKSPFDVLEQDAAKRAEEEKAAAQSGSVADRRPTKFFKGRLIAIDCSK
                     APAAILTVSSEGVVLKLRAADYKSLLLIGADDFSCAWQDRQVTVNYKPGGLADGDLVS
                     LEVR"
     gene            3408..3899
                     /locus_tag="VEJ00_03320"
     CDS             3408..3899
                     /locus_tag="VEJ00_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00219.1"
                     /translation="MNTEHQEVAQSEPSRWPTCAMLFWGTFGTKSPAERRYVMRSFFA
                     AAVVMLWMVLAFSDHFPPKPAVMKITLLATGVLITYIAWELYRYLSQLDELARRMQLE
                     AIAWTYLTGFVAAAWLGVLAPFSHFLMHWPYKPSLLTAIPFLYFLLEPVRAIWLYVLS
                     RRY"
     gene            3899..4105
                     /locus_tag="VEJ00_03325"
     CDS             3899..4105
                     /locus_tag="VEJ00_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXX00220.1"
                     /translation="MKNRLRVLRAEHNLSQGDLADRLEVSRQTVNAIETEKYDPSLPL
                     AFKIATLFGQTIEEIFTPNGDKGK"
     gene            4192..4536
                     /locus_tag="VEJ00_03330"
     CDS             4192..4536
                     /locus_tag="VEJ00_03330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXX00221.1"
                     /translation="MPLNIQTKHMQPDIVVLEMTGKITLGNDCQQVEWTTKKLVSEQQ
                     KKIIFDLSGISHVDSTGIGIIVMVAGQMKQAGGALRVAGANQHVEQMLKLTSVDQIVG
                     LHPTIAAAASTF"
BASE COUNT          999 a         1466 c         1304 g          942 t
ORIGIN      
        1 tcccggcgcg tagcgcgata aagttccgtg gctgcggcga gagcacagta gtcatcgact
       61 atgttctctt tgccgtcgtt aacgagcttc agattgtctt tttcgagatc gtcgaaggca
      121 tcttcggcag atttcaggta atcagcgctt gtaaactgtc ccgagaccgg cagcatgctg
      181 gccatggcca gcgccgcgat cgcaattcca ccgcccgagc gatagctcac ttcgtattca
      241 cgctcgttct ccgccgactc gaccatacct ttcgcgccct gacccggggc agcctcgatg
      301 ccgaaccgct tcatctcgcc ggcaatcttc cgcttctctg gaacttgatc aacacctccg
      361 ttcgataccg accgatagaa cgatccgccc ggagctttca tgcgcaccag gtagtcggcg
      421 ccgaatatgg cctcatcgag cagatgcgcc tgatagcgcg ccagctctgg cggcccatgc
      481 agcttgtttt gctcaaagct cttcagcagg ctgtacacca cgagcggaat ctgctgcgga
      541 ttgaaatacg tcgaaaacga aagatgcgaa aagtgcttgc cgtaatcgcc cgtcgcgtcc
      601 caccatccgc cgtgcgcgtc ccatgtgcct ttcttcgctg cctcgaatct cagatggctg
      661 tcggctttgt cgtattctcc cgagctgcgc tcggccttga agtagtagat gacatcctgc
      721 atcgtgttgc gctgaagcag gtttcgttgc accaggaacg gaaacgagcg cacacttccc
      781 gcttcagacg cgcactccag gtagtactga ccctcggtcg aaaacgagtc gaaatcgagc
      841 atccagaagt accagtcacg ccatttctcg accggacccg ccgctttcgc gggcacagag
      901 agaacctgct gatcgctggc ctcatccttc agcgcgcatg cggagaagct gtccgtggcc
      961 tttcccagga tcaccgcgtg cttgggtccg gccgcttcat atccgagatg gttggtgaga
     1021 actttcgcgt cactggcaag agatcgcacc gaaaaagtca gaagccagcc ggaaaatagc
     1081 agcaggcaaa gatggcgctt catgaggatc agctcccgta gcgcggttct ttcggccaac
     1141 ggcatagagt aaaccatcgt gatagcattt ttcttgaagg ccttcccttt tccagagcgg
     1201 tacgttatgc cccgcagcgg ctctatttcg ccacgacttc ttccgctttt tctgctggca
     1261 tgctcgttat cggttctggc cgccgagcaa tctccctggc tggaagttca ttccgcgcac
     1321 ttcacggtca tcaccgacgc cggcgaaaaa aaaggcaggg aagtcgcttt gcgcttcgag
     1381 cagatgcgcg ccgtctttgc ctcgctgctg ggcaaagagc gcctcaatca accggttccg
     1441 ctgaccatcc tcgccttcaa gaatgacaag atgttctatc agtgcgcgcc tctgcgtcag
     1501 ggccagccca tcgacgttcc cggcttcttt cttcccggcg aggatcagga cttcatcgtg
     1561 ctcaatctct ttgaagagga gccctggcgt gctgtcgcgc acgacttcgc gcacatgctg
     1621 ctgaactaca actatccccc cgcccaggga tggtttgacg aaggcttggc tgagtatttc
     1681 agctctattc gcgtcgatga caaaggagtc gagattggcg gcgaccccga gcttcgcccc
     1741 tcagtagcct cggatcttct tcagaatcag atcgacaccc atccgcccaa atcgctcacc
     1801 gaactgctgg gcgcgcaggt gtggctctcc attcccgacc tgttcacgat gaagcacgac
     1861 acgtctggct acaacgaggg cacgcatcac acgctgtact acgccgagtc gtggatagtc
     1921 atgcactacc tgctgcacca aaagaagctg cccgagaccg ggacttactt cgggctggtc
     1981 ctgaatcagc gcctgccggt cgaggaagcc attcagaagg cttacggtat gagttccgcc
     2041 gaactggagc aggccgtaag ggattacttc cactcccaat cttccttgct cacggcgctc
     2101 gacaatgccc gccaaaatgc cgggcacggg gagaattccg cacaggctta ccgcttccct
     2161 gctccgctcg ggcccgaaga ttcggcgatc actgcggcgc ccctccccga gtctgacgcc
     2221 cgcgctctct acgcgggagt ccaagtccgc atccctgaac gacgcgagtt cgggctgaaa
     2281 gaactgcaga cgctggcgac gaccaccaca gcagccgaca taaagttcgc agccaaggcc
     2341 gaaaaggcac aggagaaagc tgaccaggac gaaaagcagc tgccttcgac tgcggttgga
     2401 aacgccctcg cgcaccgcat ccttgcctgg gactcaatcg agcacggaga ctttgaaaag
     2461 ggcctcaccg aacttggcaa cgccgccgcc ctgaaccgga atgacatgtg gctgcgttac
     2521 tacctgtcga tgctcaaata ccgcatggca cagtcgaaac acaccgagat ccagggtctg
     2581 gccaacatga tgctcgacct gcgcgccgtg ctcgagtggt atccggaaat ggccgacgcc
     2641 tacgatctgc tggcggtcgc ccgcaacgaa ggaggcacgt cagccaacgc catgcaagcc
     2701 gagcgcgccg ccatgaacct gagtccgcgc gatgagcgct acgtctatca catggcccag
     2761 atctatattt cgggcaagca atggcaggcg gccgatgatc tcctggaccg cttgcgggga
     2821 agcaataatc cgcagatggc cgccctcgcc cgcgaaaccc agagccagat tgccaaccag
     2881 cgcaaatacg gcatgacttt gggaacagcc gctcctcaga ccaagctcgc gccgcaaaag
     2941 tcgccattcg atgtgcttga gcaggacgcc gccaagcgcg cagaggagga gaaggcggcg
     3001 gcacagtctg ggagcgtcgc agaccgaagg cctacgaagt tcttcaaggg ccgattgatc
     3061 gcaatcgatt gctcgaaggc tcccgccgcc atcctgaccg tctcttccga aggagtcgtg
     3121 ctgaaactgc gcgctgccga ttacaagtcg ctgctgctca tcggcgccga cgacttctca
     3181 tgcgcctggc aggaccgcca agtcacagtc aactacaaac ccggcggatt agctgatggc
     3241 gacctggtct cgctggaagt gcgatagggc gtcgcctact tctgtgtcgc gtcaggtgaa
     3301 tttgtcgtgc ctgccaggct cttcatgcac tctgagtctg ccgcggcatg tatattacac
     3361 ttgacaaata gtcaagcata ctttacatta ccgactgagg tgattccatg aatactgaac
     3421 atcaagaggt ggcgcagtcc gaaccgtcgc gctggcccac atgcgcgatg ctgttttggg
     3481 gcacgttcgg gacgaaaagc cccgccgagc gccgctacgt catgcggagt ttcttcgccg
     3541 ccgcggtcgt aatgctgtgg atggtccttg ctttctccga ccacttccca ccaaagccgg
     3601 cggtgatgaa aatcaccctg ctcgctaccg gtgtcctgat cacctacatc gcctgggagc
     3661 tataccgcta cctgagccag ctcgacgagc ttgcccggcg catgcagctc gaagccatcg
     3721 cctggaccta tctcaccggc tttgtcgccg cggcgtggct cggcgttctg gcgccgttca
     3781 gccatttcct gatgcactgg ccgtacaagc catcgttgct gacggcgatt ccttttctgt
     3841 acttcttgct ggaacccgtc cgcgccatat ggctctacgt cctctcgcgg agatattgat
     3901 gaagaaccga ctgcgagttt tgcgcgcgga gcataacctg tcgcagggcg atcttgcgga
     3961 caggctcgag gtatcgcgcc aaaccgtgaa cgcgatcgaa accgaaaagt acgatcccag
     4021 ccttcctctc gccttcaaga tcgccacact attcgggcag acgatcgaag aaatcttcac
     4081 accgaacggc gataagggaa agtagaggaa gctcggttac gtccgctgcc cagacgtgat
     4141 aatgttccgt gatccattcg ttggcggaga gcccaaaaag gagcttggcg catgcctctg
     4201 aacattcaga ccaagcacat gcagccggac attgtggtcc tggaaatgac ggggaaaatc
     4261 accctcggaa atgactgcca gcaggtagag tggaccacaa agaagctggt gtcggagcag
     4321 cagaagaaaa tcatctttga tctctccggc atcagtcatg tggatagcac cggaatcggc
     4381 atcatcgtga tggtggccgg ccagatgaaa caggcgggag gagcgctgcg ggtcgccggc
     4441 gcgaatcagc atgtggaaca gatgctgaag ctgaccagcg tggaccagat cgtgggtctg
     4501 catcccacga tcgcggccgc ggccagcact ttttgagcgc actgcggagc ggcattcagc
     4561 ggtccagagg cagcagataa ccgggattgg cggcaaacgc acccccgaac acctggcggt
     4621 cggcctcgcg catgtcccag gcaatcacgg atgccggcgc aatctcgatc gtgatgtcat
     4681 cccaggccgc gaacaccgga ccgatcctcg g
//
LOCUS       DASPUL010000133         2501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1389814, whole genome shotgun sequence.
ACCESSION   DASPUL010000133 DASPUL010000000
VERSION     DASPUL010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389814"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VEJ00_03335"
     CDS             complement(<1..581)
                     /locus_tag="VEJ00_03335"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00222.1"
                     /translation="MFVFHKLEDVPADFGPTLLSVGNFDGVHRAHGHVLQEIVARARA
                     RGAKAVAVTFEPHPVRILRPDSGLKLLTPTAEKLRLLEATGVDAVLLLPFGRDLSLMT
                     PRQFAERILKKKLHAREVHEGYNFRFGHKASGDVKLLTEFGRELGFDVKVYPEMTVRG
                     ERVSSSRIRQLLRDGRVNRARHLLGRPFCVLGAP"
     gene            complement(676..1449)
                     /locus_tag="VEJ00_03340"
     CDS             complement(676..1449)
                     /locus_tag="VEJ00_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXX00223.1"
                     /translation="MKATLTVLGSGTSMGVPTLGCDCAVCHSTDPRDRRTRPSIMIEY
                     GGKVVLIDTTPDFYTQAIRENITRVDAVLYTHTHADHILGIDDLRPLSYRHKPEKLPL
                     YARPDAAAFLRRMFTYIFDADYKFGGLPQLELRPIDGPVDVFGVQFEPVILIHGDAQI
                     YGYRFASAAYLTDHSEIPESSFEQLQGLDILFLDALRHKPHPTHSTVKNSLEIVDRLR
                     PKRAFFTHICHDLPHEATNASLPRHVRLAHDGLKLEFEI"
     gene            complement(1446..2042)
                     /locus_tag="VEJ00_03345"
     CDS             complement(1446..2042)
                     /locus_tag="VEJ00_03345"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HXX00224.1"
                     /translation="MAEKKKQEQSSFTVTDRRLFTADGELRDNVAEEVEPPQVATSTE
                     AKQTSPVADAAPGDAKDTFERMPPPPTSAEQEAQAAAYRQSSKDLDSEVELSGHSAKD
                     LEMTFERFLASLYMTAMLQLGLMRQQGEQPRVDLIGARQTIDTLSLIAEKTKGNLTRT
                     EENFLQNSLYELRMAYVEVTNALARPPKAGPATGTSGK"
     gene            complement(2126..>2501)
                     /locus_tag="VEJ00_03350"
     CDS             complement(2126..>2501)
                     /locus_tag="VEJ00_03350"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HXX00225.1"
                     /translation="PQETEQMQALNFNKGCYIGQEIVERIRSRGAVHRKFTGFLADSA
                     IEIAAGTKIIAGEKEIGEVTSAASLPFAGGDKTVALGYIRREIGVPGREVSIAETKAT
                     VISLPVEETLAVQPRELEHRSA"
BASE COUNT          470 a          755 c          751 g          525 t
ORIGIN      
        1 ggcgcgccca gaacacagaa gggccgcccc agcagatgcc gcgcgcggtt aacacgtccg
       61 tcgcgcagca actggcgaat ccggctgctg gaaacgcgtt cgccgcgaac cgtcatctcc
      121 gggtacacct tgacatcaaa ccccaactcc cggccgaatt cagtcagcaa tttcacgtcg
      181 ccggaggcct tgtgcccgaa gcgaaagttg taaccttcgt gcacctcgcg ggcgtggagc
      241 ttctttttga gaatccgttc ggcaaattgc cgcggcgtca tcagcgacag atcccggccg
      301 aagggaagca acagtacggc atcgactccg gtggcttcca gcaaacgcag tttctcggcg
      361 gtgggcgtca gcagcttcag gcctgaatcc gggcgcagaa ttcgcacggg atgcggttca
      421 aacgttaccg ctacggcttt cgcgcccctg gctcgtgccc gcgccacgat ctcctgcagc
      481 acatgtccat gggcccggtg cacgccgtca aaattgccga cgctaagcag ggtaggacca
      541 aaatccgcgg gcacatcttc gagtttgtgg aagacgaaca cggcagtggt cagttatcag
      601 tgatcagtgg ctggcattcg cgcagtagcg aaccaggatt gtattaactg gccactaacc
      661 actggccact gaacactaga tctcaaactc cagcttcaac ccgtcatgcg ccaaccgcac
      721 gtgcctcggc aacgaagcgt tggtggcctc gtgcggaaga tcgtggcaga tgtgcgtaaa
      781 aaaagcccgc ttgggccgca accggtctac gatctccagc gagttcttta cggtcgaatg
      841 tgtcggatgc ggcttgtgcc gcagggcgtc gagaaagaga atatcgagcc cctgcagttg
      901 ctcaaaagag gactcgggga tctcgctgtg gtcggtcagg taggcggccg aggcgaagcg
      961 atagccgtaa atctgggcgt cgccgtgaat caggatcacc ggctcgaatt gcactccgaa
     1021 gacgtcgacc ggcccgtcga tgggccgcag ctccagttgg ggaagcccgc cgaacttgta
     1081 gtctgcgtcg aaaatgtacg tgaacatcct gcgcaggaat gccgccgcgt cggggcgagc
     1141 gtacaacggg agtttttccg gcttgtggcg ataactgagc ggccgcagat cgtcgatgcc
     1201 cagaatgtgg tcggcatgag tgtgggtgta gagcaccgca tcgacgcgcg tgatgttctc
     1261 gcgaatggcc tgcgtgtaaa aatcgggagt cgtatcgatg agcacgacct tgccgccgta
     1321 ctcgatcatg atcgaaggcc gggtgcggcg gtcgcgagga tccgtcgagt ggcagacggc
     1381 acaatcacat cccagcgtag gcacgcccat cgaggtgccg ctgcccagca cggtcagggt
     1441 cgctttcatt ttccgctggt tccggtggct ggtcccgctt tcggcggccg cgccagagca
     1501 ttggtgactt cgacgtatgc catgcgcaat tcgtagagac tgttttgcag gaagttctct
     1561 tccgtccgtg tcagattgcc cttggttttt tcggctatga ggctgagcgt atcgatcgtt
     1621 tggcgcgcgc caatcagatc gacgcgcggc tgctcgccct gctgccgcat caaccccagt
     1681 tgcagcatcg ccgtcatgta cagcgaggcc aggaatctct caaaggtcat ctcgagatct
     1741 tttgcggaat gtccgctaag ctcaacctcg gagtcgagat ccttggacga ctgacggtaa
     1801 gccgccgcct gcgcctcctg ctccgccgag gtgggtggag gaggcatgcg ctcaaaggtg
     1861 tctttagcat cgccgggtgc cgcatccgcc accggtgacg tctgcttcgc ctcggtcgaa
     1921 gtcgcgacct ggggtggttc aacctcttcg gctacgttgt cgcgcagctc tccgtcggct
     1981 gtgaaaaggc ggcggtcggt tacggtgaag ctggattgct cttgtttctt cttctcagcc
     2041 ataagacttt cctaagaaaa ccgcaggttt cacccgacga tctcggcgag gcctctgcgg
     2101 tctctgcgtt caaaggttct tggcgtcatg cactacgatg ctcgagttcc cgcggctgca
     2161 ccgccagcgt ttcctcgacg ggcagcgaaa tcacagttgc cttggtttcg gcaatggata
     2221 cttcccgccc gggcacgccg atctcgcgtc ggatatatcc tagagccacg gtcttgtcgc
     2281 ctccggcaaa gggcagagaa gcagcgctgg tcacttcacc gatttctttc tcccccgcga
     2341 tgatctttgt tcccgcggcg atctcgatcg cactgtcggc caggaatccc gtgaacttgc
     2401 ggtgaaccgc cccccgcgag cgaatccgct ccacgatttc ctgcccgatg tagcatccct
     2461 tgttgaagtt cagggcctgc atctgctcgg tttcctgggg c
//
LOCUS       DASPUL010000134         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1667164, whole genome shotgun sequence.
ACCESSION   DASPUL010000134 DASPUL010000000
VERSION     DASPUL010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667164"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VEJ00_03355"
     CDS             <1..234
                     /locus_tag="VEJ00_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00226.1"
                     /translation="VSIYKTFKLTETKALRFDISSYNLFNKPQFGYPSMPDLSSLAND
                     IASGNVVNPNNFGTVTNTVNTPRQFQFGARFTF"
     gene            complement(328..1530)
                     /locus_tag="VEJ00_03360"
     CDS             complement(328..1530)
                     /locus_tag="VEJ00_03360"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXX00227.1"
                     /translation="MKRRNRRPETAAVHGASELEKKNGPVTPPIYQTSTFEVTDNEEQ
                     QRVTTTDRYYTRWGNPTITLAEQTVAAIEGTEAARTFASGMGAITTTTLALLKTGDHI
                     VAQRDSYGGVVKFLSQWLPRLGIDTTFVDTTDYEQYARAIRPNTRLLYLESPTNPALR
                     VVDFRKVAALARQHNLISMIDSTFGTPINQHPAEYGIDLVMHSGTKYLSGHADLTCGV
                     VCGRHELMEQIHETRTTLGNCMDPHAAWLLIRGMKTLAVRVARQNENAQRVAEFLQQH
                     AKVRRVHYPFLKSHPQYTTAREQMSGGGGMVTFEVEGTGEDARRVSEAMHLFTLATSL
                     GGVESLVSIPVLTSHAMISAEQRKEMGVTEQMVRLSVGIENADDLIADLEHALEAVGV
                     RQSVALSS"
     gene            1628..>2269
                     /locus_tag="VEJ00_03365"
     CDS             1628..>2269
                     /locus_tag="VEJ00_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXX00228.1"
                     /translation="MNLGLKNRVALVAAASQGIGLATAEAFAAEGCRVAICARNRQAL
                     EAAADKIRKQHGVEVFAETVDVTDAGAVSRFVAAVAARFGAIDICVTNAGGPPAKGFL
                     AASLDDWKRALEANFLSTVYLAQEVIPHMQRKHWGRIITITSITTKQPVGDLVLSNAV
                     RAAVVGLVKSLANEFGKDGILVNNVGPGFTATDRLKELAKSRSAASGKSQQEIF"
BASE COUNT          417 a          683 c          679 g          490 t
ORIGIN      
        1 gtctccatct acaagacgtt caaactgacg gaaaccaaag cgctgcgctt cgatatctcc
       61 tcctacaacc tgtttaacaa acctcagttt ggatatccga gcatgccgga tcttagttca
      121 cttgcgaacg acatagcgtc aggaaacgtc gttaacccca acaatttcgg aaccgtgacg
      181 aacaccgtca acacgccccg ccagttccag tttggcgcgc ggtttacctt ctgatagtgg
      241 acaagcagaa acaaaaccgg cctcgcaaga ggccggttct tttctttgtg agcgtccctg
      301 cattgctcac gtttccggtg atttctgcta agagctgagg gccacgctct gtcgcacgcc
      361 gaccgcctcc agcgcgtgtt ccaggtcggc gatgagatcg tcggcgttct cgattcccac
      421 ggacaaccgc accatctgct cggttacgcc catctctttg cgttgctcgg ctgagatcat
      481 ggcgtgcgac gtgaggaccg gaatcgacac cagcgattcg actccgccca ggctcgtggc
      541 cagcgtgaac aggtgcatgg cctcgctcac gcgtcgcgca tcttcgccgg tgccttccac
      601 ctcgaacgtc accatgcctc cgccgccgct catttgttct cgcgcggtgg tgtactgcgg
      661 atggcttttg aggaacgggt agtgcacgcg ccgcactttg gcgtgttgct gcaggaattc
      721 tgccacgcgc tgcgcattct cattctgtcg cgccacgcgg accgccagcg ttttcatccc
      781 gcgaatcaga agccacgcgg catgcgggtc catgcagttg cccaacgtcg tgcgggtttc
      841 gtggatttgc tccattagct cgtgccgtcc gcacacgacc ccgcaggtca aatcggcgtg
      901 cccgctcagg tatttcgtcc ctgaatgcat gaccaggtca atgccgtact cggccggatg
      961 ctgattaatg ggcgtaccga atgtgctgtc gatcatgctg atcaggttgt gctgccgggc
     1021 cagcgccgcc acttttcgaa agtcgaccac acgcaacgct ggatttgtgg gcgactccag
     1081 atagagcagc ctggtgttgg gacgaatcgc ccgcgcgtac tgctcgtagt cggtggtgtc
     1141 gacgaacgtc gtatcgatcc ccagcctcgg cagccactgt gagaggaact tgaccacgcc
     1201 accgtagctg tcgcgttgcg cgacgatgtg gtcccctgtc ttcagcaatg ccagggtcgt
     1261 ggtagtgatg gctcccatgc cggaagcgaa cgtgcgcgcg gcctctgttc cctcgatcgc
     1321 ggcaacggtc tgctcggcga gcgtgattgt cgggtttccc cagcgcgtgt aatagcggtc
     1381 ggtggtggtg acgcgctgct gttcttcgtt gtccgtcacc tcaaaggtgg aggtctggta
     1441 gatgggaggt gtgaccgggc cgttcttctt ttcgagttcg gacgcgccgt gcacggcggc
     1501 agtttccggg cgacgattgc ggcgtttcat gcttgcctcc actgggaaca gggtaattgc
     1561 cccgattcag cgcaggcaag cgaaacccgc ggcggcagcg tttataatgc gcagttcacg
     1621 ccacacgatg aacttaggac tcaaaaaccg ggtcgcgctg gtcgccgccg ccagtcaggg
     1681 gattggcttg gccacagccg aggcgtttgc cgcagaaggc tgccgcgtcg ccatttgtgc
     1741 gcgcaaccgg caggcgctgg aggctgctgc ggacaagatc cgcaagcaac atggcgtgga
     1801 ggtcttcgct gaaactgtcg acgtcacgga tgccggcgcc gtgtcccgtt ttgtggctgc
     1861 ggtcgcggca agatttggcg caattgacat ctgtgtcact aatgcaggcg gcccgccagc
     1921 caagggattt ctcgcagcca gcctggacga ctggaagcgg gcgctggaag cgaactttct
     1981 cagcacagtc tatcttgccc aggaagtgat cccgcacatg cagcgcaaac actgggggcg
     2041 cattatcacg atcacgtcga tcactaccaa gcagccggtt ggggatctcg tgctctcgaa
     2101 cgccgtccga gccgcggtgg tgggccttgt gaagagtctc gccaacgaat tcggaaagga
     2161 tggaatcctc gtcaacaacg ttggtcccgg ttttactgcc accgaccgat tgaaggagtt
     2221 ggcgaagtcg cgttcggccg cctcgggaaa atcgcagcaa gagattttc
//
LOCUS       DASPUL010000135         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1667378, whole genome shotgun sequence.
ACCESSION   DASPUL010000135 DASPUL010000000
VERSION     DASPUL010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667378"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VEJ00_03370"
     CDS             complement(<1..587)
                     /locus_tag="VEJ00_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXX00229.1"
                     /translation="MNFRGKALEFRGDVLLFSPPGIERLDRLPLLLLLHGVYGCQWNW
                     WLNGEIATIAMEMLRNGTTIPMMIAMPSDGLWGDGSGYVPHQHGNYEKWIVDDIPQCL
                     REQFPQLHPEKFLIAGLSMGGYGAMRLGMKYASRVQGISAHSSITQVAQLSRFIPFPV
                     QAFQYAGEADTDLLHWARTNLDHLPPLRFDCGTEDP"
     gene            complement(661..1605)
                     /locus_tag="VEJ00_03375"
     CDS             complement(661..1605)
                     /locus_tag="VEJ00_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXX00230.1"
                     /translation="MTRPAIGIDLGGSHASIAVVRDEKLLACQEVALDSAKPLRPALD
                     VFAAAIHKLLAELQMNTSECEGVALGFCGLADARIGRVVSTNKKYEDAPSIDFNAWCK
                     HELGMRFGIENDARMALLGERHAGAARGCDNVVMITLGTGIGGVAMIEGKLLRGKHAQ
                     AGCLGGHLPAKVGGRPCTCGAIGCVEAEASGWALPLMAREWKGFSASRLARHENINFQ
                     TLFELADAGDHVAAEIREYCLKVWATGAVGLIHAYDPERIVIGGGVMRSATVILPYIQ
                     AYVNKHAWTPWGKVQVVAAQLGNDAGLYGAIPLLTGNV"
     gene            1729..2289
                     /locus_tag="VEJ00_03380"
     CDS             1729..2289
                     /locus_tag="VEJ00_03380"
                     /inference="COORDINATES: protein motif:HMM:NF018288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HXX00231.1"
                     /translation="MNPTFAFVNWADGALQVPGLKRSEKKLGQLTGIFHDQQAWQQLD
                     PETVVYRVWWWEPVPSGTEGGLFWGITEIQPGRVGREYFMTRGHWHIIRNRAEIYGTV
                     KGTGKLVLMDRAGRTWLEDMSPGSLHYVAGEVAHRIVNTGDVPIRVTACWPSDAGHDY
                     EVADGRGFGARIIEEQGAPVLVDAKG"
     gene            2473..>2944
                     /locus_tag="VEJ00_03385"
     CDS             2473..>2944
                     /locus_tag="VEJ00_03385"
                     /inference="COORDINATES: protein motif:HMM:NF018288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HXX00232.1"
                     /translation="MYSIVMDVGRREHRAELQRRMLLFGVVVYPAGQLGNEPVRSQGH
                     IHAIAPHCGWSTPELFEIWEGHAIIYAQERADDNPGRCFAVKAGVGDKVVVPPAWAHS
                     IINADPGTSMVFGAWCDRQYGFVYDAIRAHHGLAWFPVLDVERNIHWEPNPFYRS"
BASE COUNT          619 a          872 c          841 g          612 t
ORIGIN      
        1 ggatcctcgg tgccgcagtc aaatcgcaag ggcggaaggt gatcgaggtt tgttctggcc
       61 cagtgcagca ggtcggtatc ggcctcaccc gcatactgaa atgcttgaac gggaaagggg
      121 atgaaccgcg ataactgcgc aacctgggtg atcgacgaat gagcagaaat cccttgcacc
      181 cgcgaggcat atttcatgcc taagcgcata gcgccgtaac cacccatcga cagccccgca
      241 atcaggaatt tttcggggtg caattgcgga aactgctcac gcaggcattg cgggatatca
      301 tcgacgatcc atttctcata gttgccatgc tggtgaggga cgtatccact tccgtcgccc
      361 cagagaccgt cggacggcat ggcaatcatc atcggaattg ttgttccgtt tcggagcatt
      421 tccatcgcga tggtggcgat ctcgccgttc agccaccagt tccactggca gccgtacact
      481 ccgtgcaaca gcaggagcag cggcaggcgg tcgagccgtt cgatgccggg cgggctgaac
      541 agcaagacgt cgccacgaaa ttccagtgcc ttgccgcgaa aattcaagaa gcgcaggctc
      601 tcgtttccgg ggatcgggcg cgaaagctgg gcattgggga attgttttgg catgcgcgac
      661 ttatacgttg ccggtcagca gcgggatggc gccgtagagc ccggcgtcgt ttcccagttg
      721 tgcagctaca acctgtactt ttccccaggg cgtccatgcg tgtttgttca cataagcttg
      781 aatgtaggga agtatgacag tggcgctacg catcaccccg cccccaatca cgatacgttc
      841 cggatcatag gcgtgaatca accccactgc tcccgttgcc caaaccttta aacagtactc
      901 acgaatttcg gcagcgacgt ggtcgcctgc atccgccaat tcaaagagtg tctggaaatt
      961 gatgttctca tgtcgcgcca gcctgctcgc cgaaaagccc ttccactcgc gcgccatgag
     1021 aggtagcgcc catccggagg cctcagcctc aacgcagcca atcgcaccac aagtgcacgg
     1081 gcggccgcca acctttgcgg gcaagtggcc acccaggcac ccggcttgcg catgcttgcc
     1141 gcgtagcagc ttgccctcga tcatcgctac gccgccgatc cccgtgccca gagtgatcat
     1201 caccacattg tcacaaccgc gcgcggctcc cgcatgacgt tcgccgagca acgccatgcg
     1261 tgcgtcgttc tcgatgccaa aacgcatccc aagttcgtgc ttacaccacg cattgaaatc
     1321 gattgagggc gcgtcttcat atttcttgtt ggtcgatacc acccggccga tgcgcgcatc
     1381 agccagcccg cagaatccaa gcgccacgcc ttcgcattcc gaggtgttca tctgcagttc
     1441 tgcaagtaac ttgtgaattg ccgctgcgaa aacatcgagc gctgggcgaa gtggcttagc
     1501 ggaatcgagg gcaacctctt ggcaggccag cagcttctcg tcccttacga cagcgatgct
     1561 ggcatggctg ccgcccaggt caattccaat cgcaggtcga gtcataagcg cgggaatcgc
     1621 ttcagaggat taaagaaccc catgatagtc ggtttttatt aataatgatc aatattttgt
     1681 tcttttcgtt gcattcagta actttcctca ttagcctata gaacaaatat gaaccccacc
     1741 tttgcgttcg tgaactgggc cgacggcgcg ttgcaggtcc ccggtctgaa gaggtcggaa
     1801 aagaaactcg gacaacttac cggaatcttt cacgaccagc aagcctggca acagcttgat
     1861 cccgaaaccg ttgtgtatcg agtctggtgg tgggagcccg tcccatccgg caccgagggc
     1921 ggcttgttct ggggaatcac cgagatccaa ccgggccggg tcgggagaga gtacttcatg
     1981 actcgcggac actggcacat aatccgcaat cgcgctgaaa tctacgggac agtaaagggc
     2041 accgggaaac tcgtcctgat ggaccgcgcg gggcgcacct ggcttgaaga catgagcccc
     2101 gggagcttgc actatgttgc aggtgaagtg gctcaccgca tcgtcaatac cggagacgtt
     2161 cccattcgag ttaccgcctg ctggcccagc gatgcggggc acgactacga agtcgccgat
     2221 ggccgaggat tcggcgcgcg gatcattgaa gagcagggtg ctcctgtcct tgtggatgcg
     2281 aagggctgac ccgtgaaccg cgccaaccaa gagcctgctt tccactttga tccacagcta
     2341 ggggtgcatt gccacactaa tgatctccga ttcagttacg acgaaggcgt tttcgggccg
     2401 cagccggaat tccgccgtct cgatgatatc cgccggagtt tacgagatcc cgcatgccag
     2461 ggcccggacc cggtgtactc gatcgtaatg gacgtcggcc gtcgcgagca ccgtgccgag
     2521 ctgcagcggc gcatgctact gtttggcgtt gtcgtatatc ccgccggcca acttggaaat
     2581 gagcccgtac gcagccaggg ccacatacat gccatcgctc cgcattgcgg ctggtccact
     2641 ccagagttgt tcgagatctg ggaagggcat gcgatcatct acgcgcaaga gcgcgctgac
     2701 gacaatccgg ggcgttgttt tgccgtgaag gccggcgtgg gggacaaagt agtcgtgccg
     2761 cctgcttggg cgcacagcat catcaatgca gatcccggaa ccagcatggt gttcggtgcc
     2821 tggtgcgacc ggcagtatgg attcgtgtat gacgcgatcc gggcgcacca cggcctcgcc
     2881 tggtttcctg tgctagacgt tgaacggaac attcactggg agccgaatcc tttctatcga
     2941 tccg
//
LOCUS       DASPUL010000136         9439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_704611, whole genome shotgun sequence.
ACCESSION   DASPUL010000136 DASPUL010000000
VERSION     DASPUL010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9439)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9439
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704611"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2168)
                     /locus_tag="VEJ00_03390"
     CDS             complement(<1..2168)
                     /locus_tag="VEJ00_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXX00233.1"
                     /translation="MGERVTPSGRGELARARRLKRALESGTPPAFVPPEDSSSAAQKP
                     CSATSCPIDPKAELPWHSGESTENRLQYLQQMFDISPDGLCIADLEHRARWVNETFAR
                     MFGYEAEEIAGQLLENLVVPAERVAESRWVSEAVSKGERITLETQRRKKDGNLVDVSL
                     SCAPLLLDGKIAGFYAGYHDISDRRRVEALSSALYRVAEQSSSAHDLQQFFAAVHSIV
                     DELMYARNFYLALYDPATELLSFPYFVDERDAAPAPKKLGRGLTEHLLRTGEPLLATP
                     EVLQAMEDRGEVARNGSRSLDWLGVPLKVGSHTFGALVVQTYSRNIRYGERDKEILTF
                     VARQVASAVEIKRNEQALRRSEARYRSLVQSSVYGIYRSSLEGRFLDVNPALIAMLGY
                     SSAEEVLLLDPEKHVFANPEEHTHLIEEFRRSGTLDGIEVKWKRKDGTTITVRISGRA
                     VSSADEPADVLEAIAEDVTDRRALEDQFRQAQKMEAVGRLAGGVAHDFNNLLMVISGY
                     AEVMLSKLEAEHHLRDKAQAIQQASDRATTLTRQLLAFSRKQLLELKVVDVNAIVADM
                     ERLLRPLIGENVHLVTKLAPEAGYTRADSGQLEQVLMNLVVNAKDAMPGGGKLTIQTQ
                     NTVIDESHRRGQTFIGPGRYVTLSVSDTGMGMDKETQSRIFEPFFTTKEKGKGTGLGL
                     STVYGIVKQSGGYVMVQSELGRGTTFQIYLPRTEGAAEKHA"
     gene            2384..2743
                     /locus_tag="VEJ00_03395"
     CDS             2384..2743
                     /locus_tag="VEJ00_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00234.1"
                     /translation="MASLVQRPRSATGRRGDPADQHWSMLLFESLSAGVMAVAVGFAI
                     TMAVVGVYVILIWPLTFWDLANLGLEKYGSWADTLLWSVFAGGSLAGYWFFSGAAFKA
                     KARRTNTQPTRAGGTRR"
     gene            2884..4122
                     /locus_tag="VEJ00_03400"
     CDS             2884..4122
                     /locus_tag="VEJ00_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXX00235.1"
                     /translation="MRHLGADMVRALRAWWLRFTPALLCVFAISTSSAPAQTPGVTDN
                     SLLIGSCSALDGPARFLGNQTVLGATTYLHSVNDDGGVFGRKIQLLAFDDGYDPDKAP
                     ACFKRMTKEGVFALGFFVGTPTAKVYLPMAQDEKIPVVGLFTGAQMLYEPLKHYIINV
                     RASYYDETREQVDKLWDVNLRKVGVLYQDDAFGKTVLDGVKLALQKHNATPVGLGTFA
                     RGSLEVDAGLKDVMTARPQAVVVVGPYSPVAAVVKKAHAAGWHPVFLTVSFVGTEEFI
                     KEAGQDAEGTIITQVVPPYDRLDYPTVALYRKSLTKYYPDAQPSFVSLEGFVDAMVLV
                     EGLKRAGKDLTRERFISAVESIHELNIGMGSKLVLDYGPTDHKGFDNVYPTIVSNGQP
                     VLLTDWSSVGVSAGKSGAAK"
     gene            4321..5112
                     /locus_tag="VEJ00_03405"
     CDS             4321..5112
                     /locus_tag="VEJ00_03405"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXX00236.1"
                     /translation="MTSRKSLVKLASSLLAVGLLSTVLVAETAAARYDNQIQTAVTQK
                     LAAKSQFKNVTASVEDGIVTLNGTVDLYQRKLDVAKTARKTANVQGVRNLVTVAGADV
                     PDAKLEQKLATKLRYVRVGYDSTFDYFALGVKDGVVTVEGQDRTGVGRDEAMADIANM
                     AGVKDVINNVKIEPVSTFDDAIRLRAARAIYRDSVLSKYATDPGRPIRIIVENGHVTL
                     YGSVDNATDKQIAGIRANQIFGAFSVENKLQVEGSNQTAGNQQGM"
     gene            complement(5170..5589)
                     /locus_tag="VEJ00_03410"
     CDS             complement(5170..5589)
                     /locus_tag="VEJ00_03410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HXX00237.1"
                     /translation="MPIQLSEEVKQLIDRPNFAHLATLMPDGSPNATPIWVGREGDRI
                     VVCTGEGTLKAKNMRRDPRVALSIVDFVNPYEEVQIRGRVVERRPDLDLRAMDPISHK
                     YTGKPFPFRKPEGRVALIIEVEKAKYTKLPLEHTPGK"
     gene            complement(5626..6375)
                     /gene="larB"
                     /locus_tag="VEJ00_03415"
     CDS             complement(5626..6375)
                     /gene="larB"
                     /locus_tag="VEJ00_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HXX00238.1"
                     /translation="MNAESIRKLFEQVRNGKLSPDAAVARLRHMPFEDLGFAKVDHHR
                     ALRAGMPEVILGEGKTPAQIAAIFARLAKHGGNILATRADEEQFAAVKKNVRVAEYRG
                     LARAIVLERDHTKYGKGVIAVVSAGTSDVPVAEEAVVTAELMGNEVEHFYDVGVAGIH
                     RLLANREALTRARVVIVCAGMEGALPSVVGGLVGVPVIAVPTSIGYGASFNGLAALLG
                     MLNSCASNVSVVNIDNGFGAGYVASLINRLP"
     gene            complement(6533..8125)
                     /locus_tag="VEJ00_03420"
     CDS             complement(6533..8125)
                     /locus_tag="VEJ00_03420"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HXX00239.1"
                     /translation="MSSTTNLRSRRSAAQLHALAGVEREIRAQDTHSRRKYLREFNRA
                     FRSYDSVLDSRQIQAALHEADIVLIGDYHALPAAQRYAAMLVEQRALAGDRRVVLGVE
                     TVFARDQHVLDEWWRREIDEHELRQRIRFDLDWGYDWAPFHELLVSARDHAEALYGLD
                     CMPREDLRKIGARDRHAALKIAEIRQHHPDAVIFVLFGESHLAPGHLPRALRKALPEA
                     KILTVLQNIDALYWHAAGERADKIEGVRVKNDVLCVFNATPLEKYENYRLFLDQWSRV
                     DEGPDFAPTIYNLIESLTKFLEINRYAPHNGTQPRFLVDMLPEVHGRASDAMLRRLLI
                     RKGVREAECESMLAQVEERGSAYLPQVNAFYVREFQMAHAAEDIARFLHQACQGLPHR
                     FDGPGEEIGANRAQRRSDIDTFYTGVIENAVAYFGARVLYPSHPDAPPHDSPLSRAAC
                     EKAAQAAARADKNRFDSTAQELGYRIGSQIYNAYLAGRVAPSGLRRLFLAHLDEPGLA
                     RKVCTAVIARLRSAERKAQAKP"
     gene            8229..8867
                     /locus_tag="VEJ00_03425"
     CDS             8229..8867
                     /locus_tag="VEJ00_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HXX00240.1"
                     /translation="MPNYPPQNLTVATTGASGAIFLRHFLLAVERDERVQNVNFIASD
                     SSLRVMAEELGLKGRSSLVAQILGTSSTRSGGRSASRSSRKIRPQSNADIGANVASGS
                     YPADAMVVIPCSVGTLARIANGDASHLIERAADVSLKEKRPLVLCVRETPLNKIHIRN
                     LYRAADAGATIFPLIPAYYYLPASLDEMAREFSYRVLAHLGLPQPDAFLWKG"
     gene            9091..>9439
                     /locus_tag="VEJ00_03430"
     CDS             9091..>9439
                     /locus_tag="VEJ00_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXX00241.1"
                     /translation="MPRRFFSYLIALVVLMALGAAADDRNPLAGDPKAAKAGEYEFRI
                     NCALCHGLGARGGGRGPDLTRAQKKYAHSDAEMFQIISNGIPGTAMPANGTNGQGVGM
                     TDEEIWQIITYIRS"
BASE COUNT         1765 a         2833 c         2978 g         1863 t
ORIGIN      
        1 gcgtgtttct cggcagcgcc ttcggtgcgc ggcaggtaga tctggaacgt ggtgccgcgg
       61 ccgagttcgc tctggaccat cacgtatccg ccgctctgct tgacgatgcc gtacacggtg
      121 gagagtccga ggccggttcc tttgcctttt tccttggtgg tgaagaacgg ctcaaagata
      181 cgcgactggg tctccttgtc cattcccatg ccggtgtcgc tcacggagag cgtcacatac
      241 cggccggggc cgatgaaggt ctggccgcgg cgatggctct cgtcgatgac cgtattctgc
      301 gtttgaatcg tcagcttgcc gccaccgggc attgcgtcct tggcgttcac gacgaggttc
      361 atcagcacct gctcgagctg gccggagtcg gcgcgggtat accctgcctc gggcgcgagc
      421 ttcgtgacca ggtgcacgtt ctcgccgatc agcgggcgca acaggcgctc catgtcggcc
      481 acgattgcgt tcacatccac cactttcagc tcgaggagtt gtttacggct gaaggccagg
      541 agttgccggg tcagtgtggt ggcgcggtcg gaggcctgct gaatggcctg ggccttgtcg
      601 cgcaggtgat gctcggcttc cagcttcgag agcataacct cggcgtatcc gctgatgacc
      661 atgagcaggt tgttgaagtc gtgagcgacg ccgccggcca ggcgtccgac ggcttccatt
      721 ttctgggcct ggcggaactg gtcttcaagc gcgcgccggt cggttacgtc ttcggcgatg
      781 gcttcgagca cgtcggctgg ttcatcggcg ctgctgaccg cgcggccgct gatgcgcacg
      841 gtgatggtgg tgccgtcctt gcgcttccac ttcacttcga tcccgtccag cgtgccggag
      901 cggcggaatt cttcgatcag atgtgtgtgc tcctcggggt tggcgaatac gtgcttttcc
      961 ggatccagca gaagcacttc ctcggcggag ctgtagccga gcatggcgat cagcgccgga
     1021 ttcacatcca gaaaccgacc ttccaggctc gaacgataga taccgtagac cgagctctgc
     1081 accagcgagc gatagcgcgc ctcggaacgg cgcagcgcct gctcgtttct cttgatctcg
     1141 accgcgctcg ccacctggcg cgcgacgaag gtcaggatct ccttgtcgcg ctcgccgtaa
     1201 cggatgttcc tggaatacgt ctgcaccacc agcgcgccga aggtatgact acccactttc
     1261 agggggacgc cgagccagtc gagcgatcgc gacccgttgc gggcaacttc gccgcgatct
     1321 tccatcgctt gcaggacttc cggcgtggcc agcaacggtt cgcccgtccg caacagatgc
     1381 tcggtcaatc cccggcccag cttcttgggc gcgggtgcag cgtcgcgctc atccacgaag
     1441 tacgggaagc tcagcagttc tgtagcagga tcgtagagcg cgaggtagaa attgcgggcg
     1501 tacataagct catcgacgat gctgtgcacg gcggcaaaga actgctgcag gtcatgggcg
     1561 ctgctgcttt gctcggcaac gcggtagagg gccgagctca gcgcttcaac gcggcggcga
     1621 tcggaaatgt cgtggtaccc ggcatagaat ccggcaatct tgccgtcgag cagaagcggg
     1681 gcgcaggata gggaaacgtc caccaggttg ccgtctttct tgcggcgctg cgtttccagt
     1741 gtgatgcgtt cgcccttgga cacggcttcg ctcacccatc gcgattccgc cacacgctcg
     1801 gccggcacca ccagattctc cagaagctgg cctgcgattt cctcagcttc gtaaccgaac
     1861 atgcgcgcga aggtttcgtt cacccaacgc gcgcggtgtt cgagatcggc gatgcagagc
     1921 ccgtcgggcg agatgtcaaa catctgctgc aaatactgca gacggttctc ggtactttcg
     1981 ccgctatgcc agggaagttc agctttggga tcaatcggac aggatgttgc ggagcacggc
     2041 ttctgagccg ctgaggaaga gtcttcaggg gggacaaacg cgggcggagt tccgctctcc
     2101 agcgcccgct tcaggcgccg ggcgcgcgcg agttcgcccc tgccggatgg ggtgacacgt
     2161 tcgcccattc gcggattccc ccttgaaatg ccccgtccag agggcatgtt agaacgctga
     2221 gtacctgctc gaaagttacc ccggtaatcc caaaaacaaa gcctcccaaa acggggcgca
     2281 gcccgtcggt ccttcgtaac ttggagggct cagggattgg tctttatgct ctacgctgga
     2341 ctcccgcgtg cgggctggca tgtagatttc gcgggagaat aatatggctt cgctcgtcca
     2401 aagaccgcgt tccgccacgg gccgccgggg cgatcccgcg gatcagcact ggagcatgct
     2461 gctgttcgag agcctctcgg caggagtcat ggcggttgcc gtcggctttg ccatcacgat
     2521 ggcagtcgtc ggcgtgtacg tgattctgat ctggccgctc accttctggg acctcgccaa
     2581 cctcggcctg gaaaaatacg gctcctgggc agacacgctc ctgtggtccg tgttcgccgg
     2641 aggatcgctc gcgggatact ggttcttcag cggtgcggct ttcaaggcca aggcccggcg
     2701 caccaacacc cagccgacgc gggccggcgg aacccgtcgc taggcaagcc cggagttact
     2761 ttggtgaggc gcgcaggtgc cttgcgcgtg gcacaatcac agcactttct ttcgttcgcg
     2821 aacaaactgc aaacccggcg aatcgagcct gggtgcggga aaaatcgaga ctgaggacat
     2881 gcaatgagac atcttggtgc tgacatggtc cgtgcgttgc gcgcctggtg gctccgcttc
     2941 acgcctgctc tgctgtgcgt attcgcgatt tcgacctctt ccgcgccagc ccagacgccc
     3001 ggcgttaccg acaacagcct cctgatcgga tcatgctcgg cactggacgg cccggcgcgc
     3061 ttcctgggaa atcagacggt cctgggagcc accacgtatc tccattccgt caatgacgac
     3121 ggcggggtct tcgggcgcaa gatccagttg ctggcgtttg atgacggcta cgatcccgac
     3181 aaggctcccg cctgtttcaa gcgcatgacc aaagaaggcg tgttcgcgct gggattcttc
     3241 gtgggcaccc cgacggccaa ggtgtacctg ccgatggcgc aggatgagaa aatcccggtg
     3301 gtcggcctgt ttaccggcgc gcaaatgctg tacgagccgc tgaagcacta catcatcaat
     3361 gtgcgcgcct cctattacga cgaaacgcga gagcaggtcg acaagctgtg ggatgtcaat
     3421 cttcgcaagg tcggcgttct ctaccaggac gacgcattcg gcaagacggt actcgacggc
     3481 gtgaagctgg cgctgcagaa gcacaacgcc acgcccgtcg gcctgggaac attcgcccgt
     3541 ggttctttgg aggtggatgc ggggctcaag gatgtgatga ccgctcgccc gcaggccgtg
     3601 gtggtggttg ggccctactc gccggtggcg gctgtggtca agaaggcgca tgcggcaggc
     3661 tggcatccag tgtttctgac ggtttcattt gtgggcactg aggagttcat caaggaggcg
     3721 gggcaggacg cggagggtac gatcatcacg caggtagttc cgccctacga tcggctcgac
     3781 tatccgacgg ttgctctgta tcgcaagagt ctgaccaaat actacccgga tgcccagccg
     3841 agcttcgtca gcctggaagg cttcgtcgac gcgatggtgc tggtggaggg gctgaagcgc
     3901 gccggcaagg atttgacgag ggaacgattc atctcggcgg ttgagtccat tcacgagctg
     3961 aacatcggca tgggatcgaa gctggttctc gactatggcc ccacagacca caagggattc
     4021 gacaatgtgt atcccaccat cgtgagcaac gggcaaccgg tgttgctgac ggactggtcg
     4081 agcgtcggag tcagcgcggg gaaatccggg gccgcaaagt agcgcgcgat caagacacac
     4141 gcattcccac tcttttgtag aaacccagcc tgaaacgaaa agttgtatcg acgggctgct
     4201 gatttggcaa ccgtctttct tttttctcgc cacggtgcat cctgtcccga aagcttgcat
     4261 ccaagcaatt aaccgtatgg ttaattcgta aagttaagta aacgggtcgg gaggtgacct
     4321 atgacaagca ggaagagctt agtgaaactg gcgagttcgc tcttggcggt ggggctgttg
     4381 agcacggtgc tcgtagccga gaccgcggcg gcgcgctacg acaatcagat tcagacggcg
     4441 gtgacacaga agctggcagc caagagccag ttcaagaatg tgacggcgag cgtggaagac
     4501 ggcatcgtga ctcttaacgg aacggtggat ctctaccagc gcaaactcga cgtcgcgaag
     4561 acagcgcgca aaacggcgaa cgtgcagggc gtacgcaacc tggtgacagt ggccggggct
     4621 gacgtgccgg atgcaaaact cgagcaaaag ctggcaacca agctgcgcta cgtccgcgtg
     4681 ggctatgaca gcacgttcga ctatttcgcc ctaggggtaa aggacggcgt ggtgaccgtg
     4741 gaaggccagg atcgcacagg cgtgggccgc gacgaagcga tggccgacat cgccaacatg
     4801 gcgggcgtga aggacgtcat caacaacgtt aaaatcgaac cggtttccac gtttgacgat
     4861 gcgatccgac tgcgggccgc gcgcgccatt tatcgcgact cggttttgag caagtacgcg
     4921 accgaccccg gccgcccgat tcgcatcatc gtagagaacg gccatgtcac gctgtatggc
     4981 tcggtcgaca acgcgaccga caagcagatt gccggaatcc gggccaacca gatcttcggc
     5041 gccttcagcg tggagaataa gctgcaggtc gaaggttcga accagaccgc ggggaaccag
     5101 caggggatgt aagggtggcg tacgagttga tgtagcggtg aaggaggcgc ggcgcaagcc
     5161 gcgcctttct tatttccctg gcgtgtgttc cagcggcagt ttcgtgtact tcgctttctc
     5221 gacctcgatg atcagcgcca cccggccttc gggtttgcga aacgggaacg gcttgccggt
     5281 gtacttgtgg gaaatcgggt ccatcgctct caggtccaag tcggggcggc gctccacgac
     5341 acgaccccgg atctgaactt cttcgtaggg attcacgaaa tcgacgatgg acaaagcaac
     5401 ccgcggatcc cggcgcatgt tcttcgcctt gagggtgcct tcgcccgtgc agacgacaat
     5461 gcggtcgccc tcgcggccta cccagatcgg cgtcgcattg ggtgaaccgt ccggcatgag
     5521 cgtggcgaga tgggcgaagt tcgggcggtc gatcagttgc ttcacttctt cggagagttg
     5581 gattggcatg gcagaccttt tgacgacgaa tccccgggca aagattcagg gaaggcgatt
     5641 gatcagcgag gcgacgtaac cggccccgaa gccattgtcg atattcacga cactgacgtt
     5701 ggaagcgcag gagttgagca ttccgagcag cgccgccaag ccgttgaacg acgcgccgta
     5761 gcctatgctg gtgggaacgg cgatgaccgg cacgccgacc aggccgccga cgacgctggg
     5821 cagcgcacct tccatgcccg cgcagacaat cactacgcgg gccctggtga gggcttcgcg
     5881 attggccagc aggcggtgaa tcccggccac gcccacgtcg tagaaatgct cgacttcatt
     5941 gcccatgagt tcggcagtga cgacggcttc ttcggcaacc ggaacatcgc tcgttcccgc
     6001 cgagaccacg gcaatcacgc ccttgccata ctttgtgtga tcgcgctcca gcacgatcgc
     6061 acgcgcgagt ccgcgatatt ctgcgacgcg gacgttcttc ttcactgcgg caaactgttc
     6121 ctcgtctgcg cgcgtggcca gaatgtttcc cccatgcttc gcaaggcggg cgaagatggc
     6181 tgcgatctgc gccggagtct tgccttcccc gagaatgacc tcaggcatgc ccgcgcgcag
     6241 ggcgcgatga tggtcgacct tggcgaaacc caggtcttca aaaggcatat gccgcaggcg
     6301 cgcgacggct gcgtcgggcg agagttttcc gttgcgtact tgctcaaaca gcttgcggat
     6361 ggattcagcg ttcaaaaggc gctcggcggt cggcaccgca ttcagccgtg agggttagat
     6421 tagcatttcg tggatcctga ctctgggcag cgcggggatg aacggcaaca agggagccag
     6481 cgccatgctc caggcagccg caagtcgcgg attcacgggc atccctctca ccttatggtt
     6541 tcgcctgcgc tttgcgctcg gcgctccgca atctcgcgat gacggcggtg cagactttgc
     6601 gagccaggcc gggctcatcg agatgcgcca gaaagaggcg gcgcagaccg ctcggggcga
     6661 ctcttcccgc cagataggcg ttgtagatct gacttccgat gcggtagccc agttcctgcg
     6721 ccgtggagtc gaaccggttc ttgtcggcac gcgctgccgc ctgcgcggct ttttcgcaag
     6781 ccgcccggga gagcggcgaa tcgtgcgggg gcgcatcggg atgcgacgga tacagcacgc
     6841 gcgcgccgaa gtaggcgacg gcgttctcga tcacgccggt gtagaaggtg tcgatgtccg
     6901 atctccgttg cgcccggttg gccccaattt cctcgccagg cccatcgaac cggtgcggca
     6961 atccctggca agcctgatgc agaaatcggg cgatgtcttc ggcggcatgc gccatctgaa
     7021 attcgcggac gtagaaggcg tttacctgcg gcaggtaggc actgccgcgc tcttcgacct
     7081 gcgccagcat ggattcgcac tcggcttcgc ggacaccctt gcggatgagc agccggcgca
     7141 gcattgcgtc ggaggcacgg ccgtgaactt cgggcagcat gtctacgaga aatctcggct
     7201 gcgtgccgtt gtgcggcgcg tagcggttga tctcgagaaa tttggtgaga ctctcgatca
     7261 gattgtagat agtgggcgca aagtcggggc cttcgtccac acggctccac tggtcgagaa
     7321 acaggcggta gttttcatac ttttccagcg gcgtggcgtt gaagacgcag agtacatcgt
     7381 tcttcactct caccccttcg atcttgtcgg cgcgttctcc ggctgcgtgc cagtaaaggg
     7441 catcgatgtt ctgcagaacc gtaagaattt tcgcctcggg caatgccttg cgcagggcac
     7501 gtggcaggtg tccgggggca aggtgcgatt cgccgaagag cacgaagatc acggcatccg
     7561 gatgatgctg gcggatttcg gcgatcttga gcgcggcgtg ccggtcgcgt gccccgatct
     7621 tgcgcagatc ctcacgcggc atacagtcga gaccgtagag cgcttcagca tggtcgcgcg
     7681 cgctgaccag cagctcatgg aacggagccc agtcgtagcc ccagtcgaga tcgaagcgga
     7741 tgcgctgacg aagttcgtgt tcgtcgatct cgcggcgcca ccattcgtcg aggacgtgct
     7801 ggtcacgcgc gaaaacggtc tcgactccca gaacgacgcg acggtcaccc gccagcgcgc
     7861 gctgctcgac gagcatggcg gcatagcgtt gcgcggcggg aagagcatgg tagtcgccga
     7921 tcagaacgat gtcggcctcg tgcagggcgg cctggatctg ccgcgagtcc aacacggaat
     7981 cgtagctgcg aaatgcccga ttgaactcgc gcagatactt gcggcggctg tgcgtgtcct
     8041 gggcgcgaat ctcgcgctcc acccccgcca gcgcgtgcag ttgcgcggcg ctgcggcggg
     8101 accggagatt cgtggtcgaa gacatggttt ggtcgttagt cgtgaactcc tagttcgcag
     8161 ctcatggctc gccttgactt ccgcggcgtg gcctccctat gctccaaact tccggtcctg
     8221 gaggcgtttt gccgaattac ccgccgcaaa atcttacggt cgcgaccact ggagctagcg
     8281 gagctatctt tctccggcat ttcctgctcg ccgtcgagcg cgatgaacgc gtccaaaacg
     8341 tgaacttcat agcctccgac agctcgttgc gcgtcatggc cgaggaactg ggcctcaaag
     8401 gccgttccag cctcgtcgcc cagattcttg ggacctcctc tacaaggtcc ggtggccgat
     8461 ctgcttcacg ctcgtcccgc aaaatccgac cgcaatcgaa cgccgacatc ggcgccaacg
     8521 tggccagcgg gtcctatccc gccgatgcca tggtcgtcat cccgtgcagc gtcggcaccc
     8581 tggctcgcat cgccaacgga gacgcttcgc atctgatcga acgcgccgcc gacgtttcgc
     8641 tcaaggagaa gcgtccgctg gtgctgtgcg tgcgcgaaac gccgcttaac aagatccaca
     8701 ttcgcaacct gtatcgcgcc gccgacgctg gggcgacgat ctttccgctc atcccggcgt
     8761 attactacct gcctgcttca ttagatgaga tggcgcgcga attttcttac cgcgtactgg
     8821 cccatctcgg ccttcctcag cccgatgcct ttctctggaa aggctgacgg ctcagtgact
     8881 tagcctcacc tgcagccggg aatggacccg tctactgtcg atatcggcac aacttagggg
     8941 aggattccag tgtagagagc caaatgtgcc tgaggcagat acctcacggt catgtacggc
     9001 gggatttcct ccccagcgtc ggggatttcc aaagcgcgca cagcaagggg gtagcgtaaa
     9061 atgcgggcac tttttcggaa aggcgatctc atgcccaggc gattcttctc ctatttgatt
     9121 gcgctcgtgg tgctgatggc tctgggggca gcagcagacg atcgcaaccc tctcgctggc
     9181 gatcccaaag cggcaaaggc gggcgagtac gaattccgca tcaactgcgc gctctgccat
     9241 ggcttgggag cgcgcggcgg aggccgtggt cccgacctga cgcgggcgca gaaaaagtac
     9301 gcgcactctg acgccgagat gtttcaaatc atcagcaatg gcattcccgg cactgccatg
     9361 cccgcgaacg gcacgaacgg ccagggcgtg ggcatgaccg acgaggaaat ctggcagatc
     9421 atcacctaca ttcgcagcc
//
LOCUS       DASPUL010000137         4947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1390728, whole genome shotgun sequence.
ACCESSION   DASPUL010000137 DASPUL010000000
VERSION     DASPUL010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390728"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(379..810)
                     /locus_tag="VEJ00_03435"
     CDS             complement(379..810)
                     /locus_tag="VEJ00_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXX00242.1"
                     /translation="MLICDLIKDQDTYQAELGDTVLETVRAMVERNIGAVPILHNGKL
                     VGIFSERDLMRRVVAEGRDPRSTCMAEVMTEDPLTIHTNEDIQNCLALMRRHNFRHLP
                     VCYDGHLVGMVSLRDILLHDLDEKDDEVRMMRAYIHSTPDA"
     gene            complement(1000..1428)
                     /locus_tag="VEJ00_03440"
     CDS             complement(1000..1428)
                     /locus_tag="VEJ00_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00243.1"
                     /translation="MKTILAAAIALTTVAGGLPAISGAAQQPDSASSARQESPFACNR
                     LALTPEQRKRHFDELGPVLRELKTGVHELPEGFEFEFPSDAKTYQLLAEWVGGERACC
                     PFFDIDLRSEREGGPLRLRLSGRPGTKQFIAADFSPWFQH"
     gene            1570..2019
                     /locus_tag="VEJ00_03445"
     CDS             1570..2019
                     /locus_tag="VEJ00_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXX00244.1"
                     /translation="MAQMTISAVARQVGLQASAIRYYEQIGLLPPAQRISGQRRYDAT
                     TLHRLAVVQRARQLGFTLEETRQLFFGFRRGARASERWQKLSRQKLAELESQLKAIRT
                     IQRLLREMMAKCRCDSLEQCGKGIFSSGCPDARSRTIPARRGAIPTR"
     gene            complement(2233..3732)
                     /locus_tag="VEJ00_03450"
     CDS             complement(2233..3732)
                     /locus_tag="VEJ00_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXX00245.1"
                     /translation="MSSRVAQWLQSSAAEVWFKFLLALVGLALAFGAALFSTVSRDAG
                     NVWATVILASTSLLLAALVGLITVPYLARRVAVERLRESFDYDVTRAGIVYVLVTLVI
                     GIAALNTGNNLLYIVVAAMLAAILVSGVVSAWVLRWLELDVRLPEHVFAGRAVLGRIV
                     LHNPRRFLPSFSIRVVSTRKKKKKPVKQWQWEAATFAFPFNRPPEEQWLRLPDRRLRR
                     ITLVPAPPGIFQGMAYFPYVPPRAELAADLELQFERRGRYREDSFGLATRFPFAFLTK
                     TRHVALQREVLVYPRIEPTDEFFEILPLVRGEWESFVRGRGSDLYRIREYMPEDSARH
                     VDWKATAKSGSLKVREFAREDERKLCIVFDNPEPGLISEATYEKAVDLAASLAWHFSE
                     QDAEVAFVASGRPRTRDLHEFLAWLAVIEAGKPSAGKDESGTAGSAGRGGAQGAGVLE
                     VLPEVNPNSDDYHIVITARPRGSLPGALWNSAYFVFVGDEPAVPAPGRS"
     gene            4006..4698
                     /gene="aqpZ"
                     /locus_tag="VEJ00_03455"
     CDS             4006..4698
                     /gene="aqpZ"
                     /locus_tag="VEJ00_03455"
                     /inference="COORDINATES: protein motif:HMM:NF003838.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HXX00246.1"
                     /translation="MPISKRALAEFFGTFWLVFGGCGAAVLAAGVPNVGIGYAGVALA
                     FGLTVLTMVYAVGHISGGHFNPAVSVGLVVGKRFPASELLHYIIAQVVGAIAAAGVLY
                     IIASGMTNFDAHAGFASNGYGANSPQGYSLLACFVAEVVLTAFFLIVIMGATDNRAPK
                     GFAPIAIGLCLTLIHLVGIPVTNTSVNPARSTGPAIFAGGWALQQLWMFWVAPLIGGA
                     LGGGIYKMLFGE"
BASE COUNT          939 a         1647 c         1476 g          885 t
ORIGIN      
        1 aagaccggga tgtacgttat cgcacatcga gtgacgttcg cctcaccagc tccggattac
       61 cttgccgaag ctcttggatc atgcgcaatc tttagttcag ggacttccag ctcgcctagc
      121 tacgttaact ttttccaacc cgacgttcaa tgcacccaag taaccgcgag taacgttacc
      181 gttccggtac cgggagggca aggttacttt cgtgctagaa cggccggcgt gtcggcttcc
      241 ggcgtccggc tttcggcttc cggatgggat tgcagaaggc ggagaccgca ctgcgaaatc
      301 aagacagcag caggatctac cggcttccgg gttggagttg cggacgccgg atgccggaag
      361 ccgactaagg aactcgccct atgcgtctgg ggtggagtgg atgtaggccc gcatcatgcg
      421 cacttcgtca tctttctcat ccagatcgtg cagcaggatg tcgcgcagcg agaccatgcc
      481 taccaggtga ccgtcatagc aaaccgggag atgccggaag ttatgccgcc gcatcagggc
      541 caggcaattc tggatgtctt cgttcgtgtg aatggtgagc gggtcctcgg tcatcacctc
      601 ggccatgcag gtgctgcggg gatcgcgccc ctccgccacg acgcgcctca tcaggtcgcg
      661 ttcggagaag attcccacca gcttgccgtt gtgcaggatt gggactgcgc cgatgttgcg
      721 ctccaccatc gcgcgtaccg tctcaagaac ggtgtcgccc agttccgcct gatacgtgtc
      781 ttgatccttg atcaaatcgc agatcagcat gggacacctc cggtcgaggt ccgagctgtc
      841 gaaaagcacc taccggggcg cgagtatatg ccgaaaagca agccgaaaga aagaagaaag
      901 tgcagccagt gccgtaggcg gcaccaattc gggagaatcc ccttaccggc cgcgtcttta
      961 gccctgtagc gtgggacatt gttaccccaa gtactgcgct cagtgctgga accatggtga
     1021 gaagtccgcc gcgatgaatt gcttggtgcc cgggcgcccg ctcagccgca gacgcaacgg
     1081 cccgccttcg cgctccgagc gcaggtcgat atcgaaaaac gggcagcagg cccgttcccc
     1141 gcccacccac tctgccagca attgataggt cttggcatcg gacgggaatt cgaattcaaa
     1201 gccctcgggc agttcgtgca cgccggtctt cagctctcgc agcacggggc ccagctcgtc
     1261 gaagtgccgc ttgcgctgct ccggagtgag cgccagccgg ttgcacgcga atgggctttc
     1321 ctgtcttgcg ctggaagcgg aatccggctg ctgcgcggct ccggagatag ctggtaggcc
     1381 tccagcgacg gtggtcaatg cgatggcagc ggcgagaatt gttttcatgt tcactcctgt
     1441 cggttcagtc tcgaatcggg cacaggcaat acgctacgac ttaaagtgga ctttaagaca
     1501 agcacgaaat ttgtaaaatg ttagtcatcc cggcttcccg tcgaccgtcg cacgctctag
     1561 gattccccca tggcacaaat gactatctcc gcggtggcgc ggcaggtcgg actgcaggct
     1621 tccgccatcc gctactacga gcagatcggc ctgcttcccc cggcgcagag aatcagcggc
     1681 cagcgccgct acgacgccac cacgctccat cgcctggcgg tcgtgcagcg ggcgcggcag
     1741 ctcggtttta cgctggagga aacccgccag ctcttcttcg gctttcgccg cggcgcgcgc
     1801 gcatcggagc gctggcagaa gctctcccgc caaaaactgg ccgaactcga gagccagctc
     1861 aaagccatcc ggacgattca gcggctgctg cgcgagatga tggcaaaatg ccgctgcgat
     1921 tccctcgagc agtgcggcaa ggggatcttc agcagcggat gcccagatgc ccgtagtcgc
     1981 acaatacccg cgcggcgtgg cgccattcca acccggtagt gcccagagac cttcgtcacg
     2041 gcgtcatcct gagcggagcc gtttttcagg cggagcgaag gatctcccga aacacggacg
     2101 cgaccggtcc acgggagatc cctcgccccg ctggagaaag cgcggggctt cgggatgacg
     2161 cccttgacga gggatgcagt tcagagggat caccgcaacg gaggggccac cctcgacgat
     2221 gctccgtgga gtctagctcc ggccgggcgc aggaactgcc ggctcgtcgc cgacgaagac
     2281 aaagtaggcc gagttccata gtgcgccagg caagctgccg cgcggccggg ccgtgatcac
     2341 gatgtggtag tcgtcggaat tgggattcac ctcgggcaag acttccaaaa cacccgcgcc
     2401 ctgcgcgccg ccccgcccag ccgacccggc cgtccccgac tcatccttcc ctgctgaggg
     2461 cttccctgcc tcgatcaccg ccagccacgc cagaaattcg tgcagatcgc gggtacgcgg
     2521 acgtccggaa gcaacgaagg ctacttctgc atcctgctcg gaaaagtgcc aggccaaaga
     2581 cgcggccagg tctacagctt tttcgtacgt cgcctcggaa atcagccccg gctcgggatt
     2641 gtcaaagacg atgcacagtt tgcgctcatc ttcccgtgcg aattcgcgca ctttcagcga
     2701 gcccgatttt gccgtcgcct tccagtccac gtgccgagcc gagtcctcgg gcatatattc
     2761 gcggatgcgg tacaagtccg agccgcgccc gcgcacgaag ctctcccact cgccgcgcac
     2821 cagcggcagg atctcgaaaa actcgtcggt cggctcgatc cgcggataca ccagcacttc
     2881 ccgctgcagc gccacatgcc gcgtcttggt cagaaaggcg aagggaaagc gcgtggccag
     2941 cccgaagctg tcttcgcggt agcgcccgcg ccgctcgaac tgcaactcca ggtcggcagc
     3001 cagctccgct ctgggcggga cataggggaa gtaggccatg ccctggaaaa ttcccggagg
     3061 ggcaggcacg agcgtgatcc gccgcaggcg ccggtcgggc aggcggagcc actgctcctc
     3121 cggcgggcgg ttgaagggaa acgcaaatgt ggccgcttcc cactgccact gcttgactgg
     3181 cttctttttc ttcttgcgcg tggacaccac gcgaatggaa aacgacggca gaaaccgtcg
     3241 cggattgtgc agcacgatcc ggcccagcac cgcgcgtccg gcaaacacgt gctcgggcag
     3301 gcgcacatcc agctccagcc agcgcagcac ccacgccgaa accacgcccg aaaccaggat
     3361 cgccgccagc atggccgcaa ccacgatgta gagcaggttg ttgcccgtgt tcagcgcggc
     3421 gatgccgatc accagcgtca cgagcacata gacgatcccc gcccgggtca cgtcgtaatc
     3481 aaacgattcg cgcagccgct ccaccgccac ccgccgcgcc aggtacggca cggtgatcaa
     3541 tcccaccagc gccgccaaca gcaacgaggt cgaggccaga atcaccgtcg cccagacgtt
     3601 tcccgcgtcc cgcgacacgg tcgaaaacag cgccgccccg aaggccagcg ccagcccgac
     3661 cagcgccagc aggaacttga accaaacctc cgccgccgac gattgcagcc attgcgccac
     3721 gcggctcgac atggaacggt ccgaacgcaa cgggttggcg tacgcggagc tcacagattt
     3781 cgagcctagc atcccccgct agaacggaca atcctaacca ccttgcccgg aaagcgatcg
     3841 taaccggatg ctgatccgga gagccccggg aacagtccgt gcgtcggcgg acgagacgtc
     3901 cgccgctcca cattcggggt tcgcgacctg ctttctcctg tgctaccctt tcgcgctgta
     3961 gttcactaac ccggttcaaa tctgaaacgc gcaaggagat cccccatgcc catcagcaag
     4021 cgtgccttag cggaattctt cggaaccttc tggctggtct tcggcggatg cggtgcggcc
     4081 gtgctggccg cgggcgtccc caacgtcggc atcggctacg ccggggttgc cctggccttc
     4141 gggctgaccg tgctgaccat ggtttatgcc gtcgggcaca tctccggcgg ccacttcaat
     4201 cccgcggtca gcgtgggact ggtcgtgggc aaacgcttcc ctgcttccga actgttgcac
     4261 tacatcatcg cgcaggtggt cggagctatc gcagccgcgg gtgtgctcta catcatcgcc
     4321 agcggcatga ccaacttcga cgcgcacgcc ggcttcgcct ccaacggcta cggcgccaat
     4381 tcgccccagg gatattccct gctcgcctgc ttcgtcgccg aggtcgtgct gaccgcgttc
     4441 ttcctgatcg tgatcatggg cgcgaccgac aaccgcgcgc ccaagggatt tgcgcccatc
     4501 gccatcggcc tttgcctgac cctgattcac ctggtcggca ttccggtgac gaatacctcc
     4561 gtaaacccgg cccgcagcac cggccccgcc atcttcgccg gtggatgggc cctgcagcaa
     4621 ctgtggatgt tctgggtcgc accgctcatc ggcggcgccc tcggcggcgg aatctataag
     4681 atgctgttcg gggagtgagc ctggaacgtg ccgtcatcct gagcgcagcg aaggatctca
     4741 tgtctgccga ctctgcatgc ggctcgtcag ggagttccct ccgcctgccg gggcagcgtc
     4801 gtgcgaatgg cgtgcgcttg aaattgatca gggacactcc gcgcgatccg cggcaaagaa
     4861 ctttactgat cactaaccac tgaccttagc gcctgcggtc caaccctgcc gccggaattc
     4921 ccaacgccgc gcgtgcctcg ccgacgg
//
LOCUS       DASPUL010000138         3342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1122653, whole genome shotgun sequence.
ACCESSION   DASPUL010000138 DASPUL010000000
VERSION     DASPUL010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122653"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VEJ00_03460"
     CDS             complement(<1..1180)
                     /locus_tag="VEJ00_03460"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXX00247.1"
                     /translation="MSCLRKMSRWSAVLILFWALHNSVLAQLTEGTIAGAVTDASGAA
                     VAGASVRITNLQTGSSSDTTTDNIGYYRQLHLPPGAYQVRIQARGFKTTVLENIAVDV
                     NTTTRADATLQVGAAQETVDVTEAVSLVQTEEARLSSTLTNQEITNLPLNGRQVYQLV
                     SLEPGVTQTNAPVISNVPSPTSSVTFDFGYIANGSTPRGNNFVLDGNSNNNEWLGGTP
                     LIYPSLDAIQEVQVQTLNFSAEYGRNNGTVVNVVTKSGTNNLHGSVFYTGRNTAFDTR
                     DFFNQGGKTPLQQNQFGFSVGGSIVKDKTFYFVDYEGSRLKDGAPTLFTSETPSFRQL
                     VTTNNPGSLAAMFYHDFPGPACTGPAFGVDGQYCSAVSSQLERNQADQYLVRVDQHLG
                     T"
     gene            complement(1482..>3342)
                     /locus_tag="VEJ00_03465"
     CDS             complement(1482..>3342)
                     /locus_tag="VEJ00_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00248.1"
                     /translation="IGRPTATLELVAFATRHTGEGRVDNVLLSIKSKTGSVAVVPKIH
                     IRTWEKIELESYTTPTATVSTHGSTSPLLVAAQLNNNSGTCMLWEEEGFTVYLPHGEA
                     TEEMEARYFVRLPQENQSAVTLQQHLPEADMLLAESREFWSKWKPSSATEWSYPGRHG
                     EFLTACARNIQQAREVKDGQLVFQVGPTIYRGLWIVDGNFLLEAARYLGYDQAADEGL
                     RSEWSRQVESGQIIAGSGGEHWKDTAIAMFTLVRQCELKQDWTFFRDLEPNLVRALDF
                     LISLRDQARSGQSTNGRYGLLAPGFADGGIGGVRSEFTNTVWTLAAVRAVAEAADQLK
                     MSSLAKADKFFRELYAAFQQMAKREMVRHPAGFEYLPMIAHDDPSLADPDPWNRPRPQ
                     TAQWALSHAIYPGEVFDKDDPIVRGHIALLQSCTQEDVPVETGWLWHDSLWTYNASFA
                     AHVYLWARLRDWAHRTFSGFLNHASPLYCWREEQPLQHAMVGQDWGDMPHNWATAECV
                     RYLRHMLALEDGKSLRLLNGITPAELTLSASYILRNSPTRFGRIDLELEPAGVRGLRL
                     RFARANGPSPVSVSLPQAIGALRVREVVAAGSKIAGEVVEVDPVVREWEAFLK"
BASE COUNT          716 a          960 c          963 g          703 t
ORIGIN      
        1 gcgttcccag atgctgatcg acacggacaa ggtattggtc agcctgattg cgctcgagct
       61 gagacgaaac cgcggagcaa tactggccgt ctaccccaaa agccggacca gtgcaggcgg
      121 ggccaggaaa gtcgtggtag aacatggctg ccaagcttcc cggattgttc gtggtcacta
      181 actgccgaaa cgagggtgtt tcgctcgtaa acagcgttgg cgcgccgtcc ttgagccgag
      241 agccctcata atccacaaag taaaaagtct tgtctttgac gatcgagccg cccacagaaa
      301 acccgaattg gttctgctgc aagggagtct tcccaccctg attaaagaag tctctggtgt
      361 caaaggctgt atttcttccc gtgtagaaca ctgaaccatg caggttgttg gtgcctgact
      421 tggtgacaac attaacgact gtcccattgt tgcggccgta ttcagcagaa aagttcagcg
      481 tctgaacctg gacttcctga attgcatcca gcgatggata gatgagcgga gtcccgccca
      541 gccactcgtt gttgttggaa ttgccgtcca gaacgaagtt gttgccgcgt ggggtcgatc
      601 cgttagcgat gtagccgaaa tcgaaggtga ccgacgaagt gggcgagggc acattcgaga
      661 tgaccggcgc attggtctga gttacgccgg gttccagtga caccaattga tagacctgcc
      721 gtccgttcag cggcaagttg gtgatttcct ggttcgtaag cgtactggac agccgcgctt
      781 cttcggtttg caccaggctc accgcttcag ttacgtcaac cgtctcttgg gcggcgccca
      841 cctgcagggt agcgtcggcc ctcgtcgtag tattgacatc gaccgcgatg ttctcgagaa
      901 cggtagtctt gaaaccgcgc gcctgaatgc gaacctgata cgctccgggc gggagatgca
      961 actgacggta gtagccgatg ttgtcggtcg tggtgtcaga gctggagccg gtctgaaggt
     1021 tggtgattct aacgctggct ccagcgacgg cggccccgct cgcgtcggtt accgctccgg
     1081 cgattgtacc ttccgtcaat tgcgccagca cagagttgtg gagtgcccag aacagaatga
     1141 ggactgcaga ccatctcgac atctttcgca agcaactcat gatcgcctcc agaactgagt
     1201 ttcgatcgag tgcacgacta aagatgactg caaccgccaa cgcgcgttcg cgcatgaaaa
     1261 cgaaatttcg catactttgc tggatttcag taagctttgt caaggaggtt tctcggggtc
     1321 aaatcgtcga aggaacccgc gaaacggatt gttgtcccga ccaggcaggg gcaaccaata
     1381 accacgcttg aactttgagt ctgcttctgc tgggatctga aggagagtgc cgtctcatgt
     1441 tcccttcaac ggcagaattc gtacggcacc ccggaaaaga tctacttgag aaacgcttcc
     1501 cattcccgga ctacaggatc tacctccacg acttcacccg cgatcttcga accggcggca
     1561 acgacttccc gcacgcgcaa tgcgccgatg gcttgtggca atgaaaccga aactggagaa
     1621 ggcccattgg cacgagcaaa ccgcaaccgc aaccctcgta cacccgcagg ttcgagttcc
     1681 agatcgatcc tgccaaaacg tgttggggaa ttccgcagga tgtaactagc ggacagcgtc
     1741 aattctgcag gagtaatgcc gttgagcagt cgaagcgatt tgccgtcttc cagggccagc
     1801 atatgtcgca ggtaccgaac gcattcggcg gtcgcccagt tatgcggcat atcgccccag
     1861 tcctgcccga ccatcgcgtg ttgcagcggt tgctcttcgc gccagcaata cagcggtgat
     1921 gcatggttaa ggaagccgct gaaggtgcga tgcgcccaat cgcgcagcct ggcccagaga
     1981 tagacatgcg ccgcaaatga ggcgttgtaa gtccacagac tgtcatgcca gagccagcca
     2041 gtttcaaccg ggacgtcttc ctgtgtgcac gattgcagca acgctatgtg tccccggacg
     2101 atgggatcgt ccttatcgaa cacctcgcca ggataaatgg catgggaaag cgcccattga
     2161 gcagtctgcg gacgaggacg gttccacgga tcagggtcgg cgagagatgg atcgtcgtgc
     2221 gcaatcatcg ggaggtattc gaatcccgcg ggatgccgca ccatctccct ctttgccatt
     2281 tgctgaaacg ctgcgtagag ctcgcggaag aatttgtcgg ccttcgcgag cgacgacatc
     2341 ttcagctgat cagcagcctc ggcgacggca cgcactgccg cgagtgtcca gacggtattg
     2401 gtgaactcgg agcggacgcc gccgatgccg ccgtcggcga aaccgggcgc cagaagacca
     2461 taacgtccgt tcgtactctg tcccgaacgg gcttggtccc gcaggctgat gaggaaatcc
     2521 agcgcgcgca caaggttcgg ttcgagatcg cgaaaaaatg tccaatcctg cttgagttcg
     2581 cattgccgca ccagcgtgaa catcgcaatc gccgtgtctt tccagtgctc gccaccgctc
     2641 cccgcaataa tctgtccgct ctccacctgc ctggaccatt cggaacgcaa gccttcgtcg
     2701 gcggcctgat cgtagccgag atagcgcgcc gcttcgagaa gaaagttgcc gtcgacgatc
     2761 cacaaaccgc ggtagatggt agggccaacc tgaaagacga gttggccatc cttgacttca
     2821 cgcgcttgtt gaatattgcg ggcgcaggcg gtgagaaatt ctccgtgccg gccggggtaa
     2881 ctccattctg tggcgctgga gggcttccac ttgctccaga actcgcgaga ctcggcaaga
     2941 agcatgtccg cctccggcag atgttgctgc agggtcacgg ctgactgatt ctcctgcggg
     3001 agccgcacga aataccgcgc ctccatttct tcggtggcct cgccatgcgg aaggtaaaca
     3061 gtgaatcctt cttcctccca caacatgcag gtgcccgaat tgttgttgag ctgcgcggca
     3121 acgagcaggg gcgacgtact cccatgcgtg ctaaccgtag cggtgggcgt ggtatagctt
     3181 tccagttcaa tcttttccca ggtgcggatg tggatcttcg gaaccacggc gacgctgccc
     3241 gtcttcgatt taatcgagag cagcacgttg tccacccggc cctcgccagt gtgacgagtt
     3301 gcgaaagcga cgagttccaa cgtcgccgtc gggcggccga tc
//
LOCUS       DASPUL010000139         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1801615, whole genome shotgun sequence.
ACCESSION   DASPUL010000139 DASPUL010000000
VERSION     DASPUL010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801615"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VEJ00_03470"
     CDS             complement(<1..791)
                     /locus_tag="VEJ00_03470"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXX00249.1"
                     /translation="MNMRKDEHQKHERRVIRDEIKTSAATQRAWEAWADPAKISQWFV
                     DRAEGKAEAGADITWFFDKFNMELHYKVLEAVPGERFAILWPGPMPPPGILEVIIERE
                     GGETLIRLVNSGFREGAQFDEEYEGVVSGWHTSLALLREYVENYFGEPRTNILVIQPA
                     QFEYSQVARYFREPELLGKWLTKSGSIGDTGEACKLVLRSGDALTGRVIARTEHEVAV
                     SWPEARAALELKSFSMGPQRILGIRGSGWSMDANRAQEIEKQMTEA"
     gene            complement(985..2208)
                     /locus_tag="VEJ00_03475"
     CDS             complement(985..2208)
                     /locus_tag="VEJ00_03475"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HXX00250.1"
                     /translation="MPWARTDVGSERIKFVVRAVARKERMTALCRAFGISRTTGYRWR
                     RRYLEAGNFQAVVERSRRPQRSPGQTTPALEARVVEWRQQSGWGAKKIAVLLREEGLE
                     LTVTTINRILKRRGLVDPRDGTSPALQRFARSTPNELWQMDGKGDYGARDGRCYPLSI
                     LDDHSRYAVGLYALPAFTAELIYPCLVHTFESYGVPQAMLMDRGSVWWATTNGYGLTW
                     LSVRLIEQGIQLCYGRPYHPQTQGKIERFHRTLDEAVRHRGKPQTLGEWPGALEEFRR
                     VYNERRPHEALEMQRPAQRYRPSARAYQPAPREWEYPSGSVMQQLDTGGSLSWEGRRW
                     FVCEALAGRWVRVEEVPSQSLLVSYRHMYVREIDRGRGRTRPLVMPRDRDGALRSPSG
                     LPPSPVPIATPGGEV"
BASE COUNT          401 a          871 c          598 g          432 t
ORIGIN      
        1 gcttcggtca tctgtttttc gatttcctgc gcccgattcg catccatgct ccatcccgag
       61 ccgcgaatcc cgagaatccg ctgtggaccc atcgaaaacg atttcaactc cagtgccgct
      121 cgcgcttccg gccaactcac ggccacctca tgttccgtcc gcgcaatcac ccggccggtt
      181 agcgcgtcgc cgctacgcag caccagcttg caggcctcgc ccgtatcgcc gatcgagccc
      241 gacttcgtca gccatttccc cagcaactcc ggctcgcgaa aatagcgtgc cacttgcgaa
      301 tattcaaact gcgccggctg tatcaccaga atattcgtgc gcggctcgcc gaaataattc
      361 tcaacatact cccgcaaaag cgcgagcgac gtgtgccagc ccgagaccac tccttcgtac
      421 tcctcatcga actgcgcccc ttcccggaat ccggaattga ccagccggat cagcgtctcg
      481 ccgccctcgc gttcgatgat cacctcaagg attcccggcg gcggcatcgg tcccggccac
      541 aggatcgcaa accgctcgcc tggaaccgcc tccagcactt tgtaatgcaa ctccatgttg
      601 aacttgtcga agaaccaggt aatgtccgcg cccgcctcgg ccttgccttc cgcgcgatcc
      661 acaaaccact gtgaaatctt cgccggatcg gcccaagctt cccaagccct ctgcgtcgca
      721 gcgctggtct tgatctcgtc gcgaatgacg cggcgttcat gcttctgatg ttcatccttg
      781 cgcatgttca tccctcgctc tccgtctctc aatcccgccc gctgccgtca aggcgtcttc
      841 gtaggggcgg ggcttgctcc gcccgccgct gtaatccata ccgcgaacca ggccaacttc
      901 tccgcaagcc gttttcgtcc ccgtaggggc ggggcttgct gttgggtgtc aagacatgcg
      961 ttacaccgtg tgtcaggaca tccgttacac ttcgcctcct ggagttgcga tcgggacggg
     1021 gctcggaggg agcccggagg gcgaccgcag agccccgtcc cgatcgcgcg gcatcaccag
     1081 cggccgcgtc ctgcctcgcc cccgatcgat ctcccgcacg tacatgtgcc ggtagctcac
     1141 caacagcgat tgcgagggca cttcttcaac gcgcacccag cgtcccgcca gcgcctcaca
     1201 cacgaaccag cgccgtcctt cccaacttaa actgcccccc gtatccagtt gctgcatcac
     1261 gctgccgctc ggatattccc actcgcgcgg cgcaggctgg taggcccgcg cgctcggacg
     1321 ataacgctgc gccggccgct gcatctccaa cgcttcgtgc ggccgccgct cattgtacac
     1381 gcggcgaaat tcctccagcg cccccggcca ttcccccaac gtctgtggct tcccgcgatg
     1441 ccgcaccgct tcgtccaagg tgcgatgaaa gcgctcgatc ttcccctgcg tctgcggatg
     1501 atagggccgc ccgtaacaca gctggatgcc ttgctcgatc aagcgcaccg acagccacgt
     1561 cagcccataa ccgttcgtcg tcgcccacca caccgacccg cgatccatca gcatggcctg
     1621 cggcaccccg tagctctcga aggtgtgcac caggcaggga taaatcagct ccgccgtgaa
     1681 cgccggcaaa gcatacagcc ccaccgcgta gcggctgtga tcatcgagaa tcgacaacgg
     1741 gtagcagcgc ccgtcgcgcg cgccgtagtc ccccttgcca tccatctgcc acaactcgtt
     1801 gggcgtcgag cgcgcgaagc gttgcagcgc cggagacgtc ccgtcgcgcg ggtccaccag
     1861 cccgcggcgt ttcaagatgc ggttgatggt cgtcaccgtc agctccaggc cttcctcgcg
     1921 cagcagcacg gcaatctttt tcgcacccca gccgctctgc tgccgccact ccaccacccg
     1981 tgcttccagc gctggcgtgg tctgccccgg actgcgctgc ggccgtcggc tgcgctccac
     2041 tacggcctgg aagttccccg cctccagata gcggcgccgc cagcgatacc cggtggtccg
     2101 cgaaatccca aacgcccggc ataacgcggt catgcgctcc ttccgcgcca ccgcgcgcac
     2161 cacaaacttg atccgctctg agcccacatc cgtcctcgcc cacggcatcg ttccgaccct
     2221 cctgggctcg gaactctagc gaatccgagg tgtaacggat gtcctgacac aacctgtaac
     2281 ggatgtcctg aaaccataca gc
//
LOCUS       DASPUL010000140         3829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1390962, whole genome shotgun sequence.
ACCESSION   DASPUL010000140 DASPUL010000000
VERSION     DASPUL010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390962"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VEJ00_03480"
     CDS             complement(<1..729)
                     /locus_tag="VEJ00_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00251.1"
                     /translation="MLRPLPPDRSPTLGLLLGLVITLTAVVAYSAYITWQVKGLRRLQ
                     TEMVDRNRKDSLQLLRIQNDLNLLAVAMRDMVSGDEPYPLTAWSAQFQRIRTDLDDAM
                     RQEASFAASRTPEQSAFLSSAFSQFWDAVDRVFALARDSKEREAREQIQLSLQERQAS
                     LSTAVARLLVQNSENEEQAAERIVQIYARVQRQVYIFLAATLLAILLSSLSMIRWNRR
                     LFAQLAEISERRSELAQKLIATQES"
     gene            927..2744
                     /locus_tag="VEJ00_03485"
     CDS             927..2744
                     /locus_tag="VEJ00_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXX00252.1"
                     /translation="MRPRRGLLALGFVLMVINRVSGFVLPASTKFLIDDVINKHHLAG
                     LRQLVGIVLLATVVQGITSFALTQLLSKAAQRLITELRQKVQKHISRLPVSFYDANKT
                     GSLVSRIMSDVEGIRNLLGTGLVDFAGGLLTSAIALWWLLRISPRMTLIALVSLGAFA
                     GFLSKAFSTIRPIFRERGKLTAEVTGRLTESLGGVRVIKGYHAEEREEAVFAAGVRRL
                     LDNVMRTLTATSVMSLSASVLLGLVGAATMYVGARQSLAGTLTPGQFFSFTITLPFLV
                     APMMQIVSIGTQLTEALAGLERTQEILRERPEDRDPKRVVVLSDVIGTVEFDRVSFSY
                     DGEHEVLHGISFLSEPGTVTALVGSSGSGKSTTIGLISAFYVPTMGTVRVDGVDLSTV
                     RLDSYRTRLGVVLQESFLFDGTIRENVIFSRPDASEEEFMRACRIARVDEFAEAFADK
                     YETIVGERGVKLSGGQRQRISIARAILAEPRILILDEATSSLDSESEEMIQQGLSYLM
                     QGRTTFVIAHRLSTIRRADQIMVMEQGQIVERGTHSQLYAARGRYYDLYTRQYGVEHN
                     LFLAPGEGDTVAANGDGADTKNGDMKAGDALRVLRGDIR"
     gene            2750..3514
                     /locus_tag="VEJ00_03490"
     CDS             2750..3514
                     /locus_tag="VEJ00_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXX00253.1"
                     /translation="MVTLPEAIPETRTVAIRSENLCRHYRMGEALIRAVDGISLEVRA
                     GEFVALLGSSGSGKSSALNLIAGLDRPTSGTVMVHDRDLAKLSREELAKYRLHVVGMV
                     FQSFNLIASMTIAENVELPLRFAEIERGRREDLVREALQRVGLGARMNHRPSELSGGE
                     QQRAALARALINRPQLLLADEPTGNLDSHTGSEIMDLVREFNKQLGMTVVMVTHERAL
                     AERYAERMIFLADGKLIDDRANVPGTAHSGPQRGRP"
     gene            3511..>3829
                     /locus_tag="VEJ00_03495"
     CDS             3511..>3829
                     /locus_tag="VEJ00_03495"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX00254.1"
                     /translation="MKIPDLTELALRNLRESLLRNSLTTVGISVGVASLVAMLSLGTG
                     LQQLFSRRLAKSGLFDTVLVSSRRELHGMSREEERNGPSPGASRILDEPARQEIERLP
                     NVAE"
BASE COUNT          735 a         1113 c         1212 g          769 t
ORIGIN      
        1 ggattcctgg gttgcgatca gtttttgcgc cagctcgctg cggcgctcgg agatttccgc
       61 cagctgcgcg aacaatcgcc ggttccagcg aatcatcgag aggctgctca gcaggatcgc
      121 aagcagggtt gctgcaagaa aaatgtagac ctgccgctgc acccgggcgt agatctgcac
      181 aattctctcc gcggcttgtt cttcgttctc gctgttctgc accagcaacc gggcgacggc
      241 cgtgctgagc gaagcctgcc gctcctgaag agagagctgg atctgttccc gggcttctct
      301 ttccttgctg tcacgagcca gagcgaagac ccggtcgacg gcgtcccaga actgggaaaa
      361 cgcgctcgag agaaaagcgc tctgctccgg tgtacgactg gcggcaaagc tcgcttcctg
      421 ccgcatcgcg tcatccaggt cggtccgaat gcgctgaaac tgcgccgacc aggcagtcag
      481 aggatagggc tcatcgccgc tgaccatgtc gcgcatggcc acggccagca ggttgagatc
      541 attctggata cgcagcaact gcagcgaatc cttgcggttg cggtcaacca tttcagtctg
      601 caaccggcgc agccctttca cttgccatgt gatgtatgca gaataagcga cgacggccgt
      661 tagcgtgatg accaggccta atagaaggcc caaagtcggc gaacgatcgg gtgggagcgg
      721 gcggagcacg cccccgagca tacaacgggc cgacggcgcc tgtcacaagc agcgctggaa
      781 gggcggatgt gtgacaatgc agtaataatg aagcgcaaac acgctccgcg gagtcaaaca
      841 gcagattgag tcctccagcg aaagaagccc cgaaaccccc gaagatccag cgtctgaaga
      901 ccattttgcc cgaggtctgg agtctgatgc gtccccgccg cggactgctc gcgctgggct
      961 tcgtgctcat ggtgatcaat cgcgtgtcag gatttgtgct tccggcgtct acgaagtttc
     1021 tgatcgacga cgtcatcaac aagcatcatc tggcgggatt gcggcagctg gtcggaatcg
     1081 tgctgctggc gaccgtggtg caagggatca cctcgttcgc gctgacgcag ttgctctcca
     1141 aggctgcgca acgcctgatc accgagctcc gccagaaggt gcagaagcac atttcgcgcc
     1201 tgccggtatc gttttacgac gcgaacaaga cgggcagcct ggtgtcgcgc atcatgagcg
     1261 acgtggaggg cattcgcaac cttctgggca cgggcctggt ggatttcgcc ggcgggttgc
     1321 tgacttcggc gatcgccctc tggtggctcc tgcgtatcag cccacgcatg acgctgattg
     1381 cgctggtatc gctgggggcg tttgccggat tcctgagcaa ggcattttcg accatccggc
     1441 cgatctttcg cgagcgcggc aaactcactg ccgaagtgac gggccgcctg acggaatcgc
     1501 taggcggcgt gcgtgtcatc aagggatacc acgccgagga gcgggaagaa gccgtctttg
     1561 ccgctggagt gcgcaggctg ctggacaatg tcatgcgtac cctgactgcg acgtctgtga
     1621 tgagcttgtc ggcgagcgtg ctgcttgggt tagtcggcgc ggcgacgatg tacgttggcg
     1681 cccggcagag ccttgccggc acacttactc ccggacagtt tttctcattc accatcactc
     1741 tgccgtttct ggtcgcgccc atgatgcaga tcgtatccat cggcacccag ctcaccgagg
     1801 cactggccgg gctcgaacgc acgcaggaga tcctccgtga acgaccggag gaccgggatc
     1861 caaagcgcgt cgtggttctc tccgatgtca tcgggacggt ggagttcgac agggtgagct
     1921 tcagctacga cggagagcac gaggttttgc acggcatctc cttcttgtca gagcccggga
     1981 cggtgaccgc cctggtggga tcttcggggt cgggaaaatc gacgacgatt ggcttgattt
     2041 ccgccttcta tgttccgacc atgggcacgg tgcgggtgga cggcgtggac ttgagcaccg
     2101 ttcgtctcga ttcttaccgc acgcggcttg gcgtcgtgct gcaggagtca tttctgtttg
     2161 acggaacgat ccgcgagaac gttatctttt ctcggccaga cgccagcgag gaggaattca
     2221 tgcgcgcctg ccgcattgcg cgtgtcgacg agttcgctga agcgtttgcc gacaagtatg
     2281 agaccatcgt cggagagcgc ggcgtgaagt tgtcgggcgg tcagcgccag cgcatctcga
     2341 ttgcccgcgc catcctcgcc gagccgcgga ttctcattct cgacgaggca acttccagtc
     2401 tggactcgga atccgaagag atgatccagc aaggtctctc ctacctgatg cagggccgca
     2461 ccacgttcgt catcgcgcac cggctttcga ccatccggcg cgcggatcaa atcatggtca
     2521 tggaacaagg acagattgtc gaacgtggga cacacagcca gctctatgcc gcccgcgggc
     2581 ggtactacga tttgtacacg cggcaatacg gagtggagca caacttgttc cttgcccccg
     2641 gcgagggtga cacggtcgcg gcgaatggcg atggggccga caccaaaaac ggggatatga
     2701 aggcgggcga cgccctgcgc gtcttacgag gagacatacg ctagccagta tggtgaccct
     2761 gcctgaggct attcctgaaa cccgaacggt tgcgattcgg agcgagaatc tctgccggca
     2821 ttaccgcatg ggcgaagcgt tgattcgcgc ggtcgacgga atttcgctcg aggttcgtgc
     2881 gggagaattt gtcgcgcttc taggcagttc gggatcgggc aaatcctcgg cgctgaacct
     2941 gatcgccggg ctcgatcggc ccacctcggg gaccgttatg gttcatgacc gtgatctggc
     3001 aaagctctcg cgcgaagaac tcgccaagta ccggcttcat gtcgtcggaa tggtctttca
     3061 gtcctttaat ctgatcgcaa gcatgacgat cgcagagaat gttgagcttc ccctgcgctt
     3121 cgcggagatc gagcgcggaa gacgcgagga tctggttcgc gaagcgctgc agcgcgtggg
     3181 gttgggagca agaatgaatc accggcccag cgaactttcc ggcggggagc agcaacgcgc
     3241 ggcgctggcg cgcgccctga tcaatcgtcc gcaactgctg ctggcggatg agcccaccgg
     3301 gaatctcgac agccataccg ggtccgagat catggacctg gttcgcgagt tcaacaaaca
     3361 actcgggatg accgtcgtga tggtgaccca cgagcgtgca ctggccgaac gctatgccga
     3421 gcgcatgatt tttctcgccg atggaaagct gatcgatgac cgggccaatg ttccgggaac
     3481 ggcgcattca ggtccgcagc gagggcgacc gtgaagattc ctgatcttac cgaactcgcc
     3541 ctgcgcaacc tgcgggagtc tttgctacgc aattcgctta ctacggttgg aatctcggtc
     3601 ggcgtggcct cgctggtggc aatgttgtcg ctaggtaccg gtttgcagca actattttcg
     3661 cgacgactgg cgaagtcggg tctgtttgat acggttctgg tttcttcccg gcgggagctc
     3721 cacggcatga gccgcgagga ggagcgtaat ggtccaagtc ccggcgcaag ccggattctg
     3781 gacgagcccg cgcgtcagga gatcgagcgc ctgccgaatg ttgccgaag
//
LOCUS       DASPUL010000141         3443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1254354, whole genome shotgun sequence.
ACCESSION   DASPUL010000141 DASPUL010000000
VERSION     DASPUL010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254354"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VEJ00_03500"
     CDS             <1..241
                     /locus_tag="VEJ00_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00255.1"
                     /translation="EAMFWVGVTHSRCQEVLSRDAPADWTQSFKENYQELVSDLGVPT
                     LAEVRRRCAEIGQTLPQVCDLAEGIIAANHEIDPD"
     gene            complement(401..1918)
                     /locus_tag="VEJ00_03505"
     CDS             complement(401..1918)
                     /locus_tag="VEJ00_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXX00256.1"
                     /translation="MRLVRVACAALNLATFEWTIASGLARSGSNPAAPAIEADAFAPG
                     GCRAGDVNDFAESAKALYDSREPAKMLANLEGISIEAAFVLKDLHRHMDDPVVVRRLR
                     DVGQKFATNRRTVILTAPKISIPPELASLVEFLQLPLPDRQRLRQIIDEVLVRISKNH
                     TLQRRLEPAGLDAMADNLRGLTEEEAERAISQALVTRYALCPETVTDVLDAKKTLLKR
                     SEMLDFVEASDTLASVGGLDNLKRFLAQRRGSWEDAARAFGLDPPRGVIILGVQGCGK
                     SLCARAIAGEWNLPLVKFDTAAIYDKYIGETEKRIQKVFQVAEGLAPCVLWIDELEKV
                     FAGSSPDSASVDAGVSSRLLASFLSWMQDRKAPVFVAATCNNVNALPPELIRKGRFDE
                     LFFVDLPNQAERKQIFSLQLSKRKRTPADFNLDRIAAAARGFSGAEIESVVQTALYAA
                     YSQKQQLASDHLLQAIQGTVPLSITRAEEIEALHDWAEKRAVPATAPQALSSQAN"
     gene            2550..>3443
                     /locus_tag="VEJ00_03510"
     CDS             2550..>3443
                     /locus_tag="VEJ00_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00257.1"
                     /translation="MARQWMVEEASGIRGFCGAYTAGSTNWLPDDADLSTASDLDIMV
                     VLADPNQAGRRGKFIYHDTLLEVSYLRDDQLQSPDRVLRDYHLAPSFRTTKIMFDPLG
                     HLTPLLAAVSRDYNKRRWVRQRCTNAKYKILGYLRSIHEKAALHEQVIACLFAAGITT
                     HILLVAGLRNPTVRARYVAVRELLADYGQVEFHERLLELLGSVRISRERVGRHLATLT
                     EIFDRAKGAIKSPFPFVSDVSDSARPVAIDGSLGLIGRGYHREAMFWVGVTHSRCQEV
                     LSRDAPADWTQSFKENYQELVS"
BASE COUNT          671 a          922 c         1147 g          703 t
ORIGIN      
        1 ggaagcgatg ttctgggttg gcgtcaccca ttccagatgt caggaggtcc tctcccgcga
       61 cgcaccggca gattggacgc agagtttcaa agagaactat caagagctgg tcagcgacct
      121 cggcgtgcct acactcgcgg aggttcggcg gcgctgtgca gagatcggac aaaccctccc
      181 ccaagtgtgc gacctggcgg aaggcattat cgcagcgaac cacgaaatcg accctgattg
      241 atgggcgcgt cccccttttt gtccccgtaa ccttcagcgg gtccctgtcg tcgtggtgaa
      301 ctcgcaatcg ccaccccagg gcagacactt gacttcgttc ggctcgcgcc tcaggtcgct
      361 cggggacagg ggtgattttc cttaaagacc ttgatttttc tcaattcgct tgcgacgaca
      421 aggcttgggg ggcggtcgca gggacggcgc gtttttcggc ccaatcgtga agggcttcga
      481 tttcttcggc gcgggtgatg gagagcggaa cggtgccctg gatggcctgg agcagatggt
      541 cgctggcgag ttgctgtttt tgcgagtagg cggcgtagag cgcggtctgg acaacggatt
      601 cgatttcggc accggagaag ccgcgggcgg cggcggcgat ccgatcgaga ttgaagtcgg
      661 cgggagtgcg ttttctcttg ctgagttgga gggagaagat ttgcttgcgt tcggcctggt
      721 tgggtaagtc cacgaagaaa agttcgtcga agcggccttt gcggatgagt tccggcggga
      781 gcgcgttgac gttgttgcag gtggcggcga cgaagacggg ggccttgcgg tcctgcatcc
      841 aagagaggaa cgaggccagc agacgggagg agacgccggc atcgaccgag gcggagtctg
      901 ggctgcttcc ggcgaagact ttttcgagtt cgtcgatcca caggacgcag ggggcaaggc
      961 cttctgcgac ctgaaagact ttttgaatgc gcttttcggt ttcgccgatg tacttgtcgt
     1021 agatggcggc ggtgtcgaat ttgaccagcg ggaggttcca ctcgccggcg atggcgcggg
     1081 cgcacaggct cttaccgcag ccttgaacgc caaggatgat cacgccgcga ggaggatcga
     1141 ggccgaaggc gcgggcggca tcttcccagg atccgcggcg ctgggcaaga aagcgcttga
     1201 ggttgtcgag tccaccgaca ctggcgaggg tgtcggaggc ttcgacgaaa tcgagcatct
     1261 cggaacgctt gaggagcgtt ttcttggcat cgaggacgtc ggtcacggtc tcggggcaga
     1321 gggcgtagcg ggtgaccagg gcttgcgaga tggcgcgctc ggcttcctct tcggtcagac
     1381 cgcggagatt atccgccatt gcgtcgagac cagcgggttc gaggcggcgt tgcagggtgt
     1441 ggtttttcga aatacgtact aaaacttcgt cgatgatctg gcggaggcgc tggcgatcgg
     1501 gcagggggag ttggaggaac tccaccaggc tagcgagttc gggcgggatg gaaattttcg
     1561 gggcggtgag gatcacagtg cggcggttgg tggcgaactt ctggcctacg tcgcggaggc
     1621 ggcgaacgac gacgggatcg tccatgtggc ggtggaggtc tttcaggacg aaggcggctt
     1681 cgatggagat gccttcgaga tttgcaagca tttttgcagg ttcacggctg tcatagagcg
     1741 ccttggcgct ctcggcgaag tcattaacgt cgccggcacg gcagcccccg ggagcaaagg
     1801 cgtcggcttc aatggcgggc gcggcggggt tggagccgga gcgggcgagg ccgctggcga
     1861 tggtccattc aaaggtcgcg aggttcaggg cggcgcaggc gacgcgcacc aggcgcatgg
     1921 cgcggacttc ttcgacggtc tccatgacca cgatgggcgt ggaggagtcg attaggacct
     1981 tgaggcgatc gaggaccttg tccatatccg ttatgaccgg tcagcagttg ctctcgtaca
     2041 ataacgtatc cggtacccac cagaacacct cgctcataga aacgaaggta atcagcgtca
     2101 tttatcggcg ctgaggcgcg cccgtcagac ggagaaacgc attgcgggcc gaaggcccca
     2161 gatcacagtt ttgaatgaca cacgtgcctt ttaggggctg tgtagggaat agaaagcccc
     2221 cgagaccccc gtatactgaa gactaaactc catcgtgggg tgatttaggt tgaggttgaa
     2281 ctagctctga aaggctggcg tcggcggttc caccccgccc tggcctccag gtttgaagcc
     2341 ctcattaacg aatcggcgtg cagtccttcg atgggagttc ggaaaaaggg gccgtgacgc
     2401 cgctatttga tatcgagttc cttgagcttt tctagccggc gcgccaaccg gctttgtccc
     2461 ctcgcactac gatcggcccc tccctggaca gcgccgggaa aaatcgggca cgctttaggt
     2521 gaactatgac ggtcaaggaa gccagagagg tggcccgaca gtggatggtt gaggaggcca
     2581 gcggtatacg gggattctgc ggagcttaca ctgccggctc aacgaactgg ctccccgatg
     2641 acgcagacct gagcacggca tcggatctcg atatcatggt cgtcctcgcc gacccgaacc
     2701 aggctggcag gcgtggcaag ttcatctatc acgacaccct tctggaagtc tcttacctgc
     2761 gggacgatca gctccagtca cctgaccggg tcttgaggga ttaccatcta gctccgagtt
     2821 ttcggacgac gaaaatcatg ttcgatccat taggccatct gactccgctc ctggccgccg
     2881 tgtctcggga ctataacaaa cgacggtggg tccgtcagcg ttgcaccaac gccaaataca
     2941 agattctggg atatctgcga tcgatccacg agaaagcggc ccttcatgaa caggtgatcg
     3001 cctgcctttt cgcggcaggc ataacaaccc acatcctgct ggtagcgggc ctgaggaatc
     3061 ccaccgtacg cgcgcggtac gtggcggtgc gagagctatt ggctgactat ggtcaggttg
     3121 aattccacga aaggctactg gagttgctgg ggtctgttcg aattagccga gagcgagttg
     3181 gccggcatct tgccaccctc acggagatct ttgaccgggc caagggagcc atcaagtcac
     3241 cgtttccttt cgtatccgac gtcagcgaca gcgcccgccc ggttgcgatc gatggaagct
     3301 tagggctgat cgggcggggc taccatcggg aagcgatgtt ctgggttggc gtcacccatt
     3361 ccagatgtca ggaggtcctc tcccgcgacg caccggcaga ttggacgcag agtttcaaag
     3421 agaactatca agagctggtc agc
//
LOCUS       DASPUL010000142         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_705583, whole genome shotgun sequence.
ACCESSION   DASPUL010000142 DASPUL010000000
VERSION     DASPUL010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705583"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VEJ00_03515"
     CDS             <1..429
                     /locus_tag="VEJ00_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00258.1"
                     /translation="IAIVYGLLWSKATWQGAMAGYIAGAIAGATLRFGFHFEVALVTL
                     ISGGVALVVCPVASLMTNAAGSTSSWFQTVKAENPIIAVGDRRSLIARRVGSWILAIG
                     FLTFLAGVLMGSQAAGHASAVAITGMLIFFAGGALRAKFV"
     gene            complement(451..1251)
                     /locus_tag="VEJ00_03520"
     CDS             complement(451..1251)
                     /locus_tag="VEJ00_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HXX00259.1"
                     /translation="MDPQKQKNGKRLMADERKRHILELLNAEGRITVQELVEKLQVTA
                     VTARADLDGLAGSGLLIRSRGGALKADGFVPEYPVSIRDRLHKPEKQRIAKAAVELIR
                     PNQTVLLDSGSTTMELARQIRASNIRPLTVITNSVQIATELAGLPEISIVLPGGILRP
                     NSYSMIGSYAERTLRELSADHAFLGVDGLDPSHGLCTTNLLGAQVDALMIQIAREVTI
                     VADSTKIGRRGLAVIAKISAIHRLITDRGADRRLIAEIEAQGVKVQLV"
     gene            1474..>2202
                     /locus_tag="VEJ00_03525"
     CDS             1474..>2202
                     /locus_tag="VEJ00_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HXX00260.1"
                     /translation="MAKHIERLSRRNRTRLPQKHGQDACAWLRDVLRNNRESGKGGTY
                     AVCSAHLAVIEAAIQQSVHDGSVLHVESTSSQVNQFGGYTGSTPSQFANLIRSLAEKA
                     GLPANRTLLGGDHLGPFPWRSEASASALSKACELVRDCVLAGYQKIHLDASMACADDP
                     RSGPPEKTIAERAAILCRAAEEALLKLPSTTPPLVYVIGTEVPAPGGESVDAHAPAVT
                     TAEHVHQTMETFRLAFVQQGQSSAW"
BASE COUNT          418 a          636 c          675 g          473 t
ORIGIN      
        1 atcgccatcg tatatggatt gctgtggagc aaagcgacct ggcaaggtgc gatggcaggg
       61 tacattgcgg gagcgattgc cggggctact ctgcggtttg gctttcactt cgaagtggcg
      121 ctcgtgacgc tgatcagcgg aggcgtcgcg ctggtggtgt gtcctgtcgc cagtctgatg
      181 accaacgccg ccgggagtac gtcttcctgg tttcaaaccg ttaaagcgga aaatcccatc
      241 atcgccgtcg gcgaccgacg aagtctgatc gcgcgacgcg tcggaagctg gatcctcgca
      301 attggttttc tgacgttcct ggctggagtc ctgatgggaa gccaagccgc cgggcacgct
      361 tccgcggtgg cgatcaccgg catgctgatc ttttttgcgg ggggcgcgtt gcgggcgaag
      421 ttcgtttgaa agccgcgagt cagcagactg tcaaaccagt tgcactttta cgccctgcgc
      481 ttcgatttcc gcaatcagcc gtcgatcggc accgcgatcg gtaatcagcc gatgaatggc
      541 gctgattttg gcaatgacag ccagaccgcg cctgccgatc ttggttgaat cggcgacgat
      601 cgtaacctca cgggcaatct gaatcatcag ggcatcgacc tgcgccccca gcagattggt
      661 ggtgcacaac ccatgcgacg gatccagccc gtcgacccca agaaatgcgt gatcggcgct
      721 gagttcgcgc agcgtgcgtt cggcgtaact gccgatcatg gaatacgaat tcggccggag
      781 aatccctccg ggaaggacaa tcgagatctc gggcaaccct gctaattccg tggcgatctg
      841 caccgaattg gtgatgacgg tgaggggacg aatgttcgag gcacggatct ggcgggccag
      901 ttccatggtg gtgctgcccg aatccagcag aacggtctgg ttcggccgaa ttagttcgac
      961 agcagctttg gcgattcgtt gcttttccgg tttgtgcagc cggtcgcgga tggagacagg
     1021 atattcgggc acaaacccat ccgccttcag agcgcctcct ctggatctga tcagcaggcc
     1081 tgagccggca aggccatcga ggtccgcgcg cgcagtgacc gcggttacct ggagcttctc
     1141 tacgagttct tgtaccgtga tccgcccttc ggcattcaga agttcaagga tgtgcctttt
     1201 cctttcatcg gccatcaggc gcttgccatt cttttgcttt tgcggatcca cttgggcagt
     1261 gctccgttcc ccggcgggtt cagtgtagcg ccgggtgctg ctatttgtct gctgttattg
     1321 gatttatgcc tgctccagga ggcaagggca gcgttgtttc gttatgctat gctttctttc
     1381 gattaatatc ctttgggggc ggcgagctgt cgaatctgaa cgcaaaaggt cgagaacctc
     1441 aagcagcgac aggtctgtag ggcgaccacg cagatggcca aacatatcga acggctctct
     1501 cgccgcaaca ggacgcgact cccgcagaag catggccagg acgcgtgtgc gtggcttcgc
     1561 gatgttctac gaaacaatcg cgagtcggga aagggtggaa cctatgcggt ctgctccgct
     1621 cacctggcgg tcatcgaggc ggccatccag cagtcggtgc acgatggttc ggttctgcat
     1681 gtcgagtcga cgtcaagcca ggtgaaccag tttggcggct acaccggctc cacaccaagt
     1741 cagtttgcga acctgatccg ctcccttgca gagaaggcag gactgccagc caacaggacc
     1801 ctgcttggcg gcgatcacct cgggcctttc ccatggcgaa gcgaagcatc ggcctccgcg
     1861 ctgagcaagg cctgcgagct ggtgcgcgac tgcgttctcg cgggctacca gaagattcat
     1921 ctggatgcga gtatggcttg cgccgacgac ccgaggagcg gtccgccgga aaagaccatt
     1981 gccgagcgcg ccgcgatttt gtgccgtgct gcagaagaag ctcttctgaa actaccctca
     2041 acgactccgc cacttgtcta cgtgatcggg accgaagttc ccgcgcccgg tggagaatca
     2101 gtcgacgcgc atgcccccgc cgtgaccacc gccgaacatg tgcaccagac aatggaaact
     2161 ttccggcttg ccttcgtgca gcaggggcaa tcgtccgcgt gg
//
LOCUS       DASPUL010000143         3981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1801758, whole genome shotgun sequence.
ACCESSION   DASPUL010000143 DASPUL010000000
VERSION     DASPUL010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1801758"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2162
                     /locus_tag="VEJ00_03530"
     CDS             <1..2162
                     /locus_tag="VEJ00_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HXX00261.1"
                     /translation="VGMIPPAWSEDDASQRPVELQVTAVSAHTFRLSILPVNNGEIGT
                     IPANGSLVQESWGSPETFRTAPKGEFAVAGCRLTISLNPVRIKIANERGNAIQQLDWD
                     ANSGQLSFLSGNQPLFGMGEGGPQFDRRGSVDRMKSGQGGYKLATHGGRVPIPWIIGT
                     SGWAMFIHQPFGTFDFTGPVCKFQPASPDSALPLDLFFIASADPATIMAEYARITGNP
                     EKPPLWSFGYQQSHRTLASREEVLAEADTFREKKLPCDTLIYLGTGFCPSGWNSENGS
                     FVWNSRVFPDPKDVLDQLHKKNFRAVAHVVILSDRLRGTVHDPCDVSRFDEELASCHW
                     DQHRKDFAMGIDGWWPDEGDPLDIASRLVRNRMYWEGPQIDRPNERPFALHRNGYAGM
                     QRYASFLWSGDVYSTWETLKTHLPIALNTGLSGIPYWGTDIGGFVPTREFTAELYVRW
                     FQFGAFCPLFRCHGRTWKLRLPWGWNTGDPGPVEINNYNGAAIPDPSQLHDARVEPIC
                     RKYLELRYRMLPYLYSAVHESTTTGIPIMRALWLHFPDDPKAVQCEDEYLWGKSVLVA
                     PVVEKGATSRRVYLPPSAWYDFWTGERLEGGREISRPVDLETMPLYVRAGSLLPLGPV
                     KQYTGEKVEGPLSISIYPGADASFLLYEDDGISFSYRSGEWMGIQMSWDEKRRMLSLQ
                     LAPGSRMLPPGPRPITAQLGEIRRSVAFEGKPIDLSF"
     gene            2199..3182
                     /locus_tag="VEJ00_03535"
     CDS             2199..3182
                     /locus_tag="VEJ00_03535"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXX00262.1"
                     /translation="MKYRPLGRTGWNVSEVSFGAWAIGGAWGKVSEKDALAALHQAVD
                     SGANFIDTADVYGNGRSERLIAQLRKQRTETIVVATKAGRRLPRQTVEGYSRKNLTSW
                     VEDSLRNLSADALDLLQLHCPPTELYYQPEVFAILDDFVKAGKIRYYGVSVEKIEEAL
                     QALQWPNVQSVQIIFNCFRQRPAEVFFARAKEKRVGILARVPLASGLLTGKLQATSTF
                     PADDHRNFNRHGEAFDVGETFSGVDYDLGLQAVEEIRRLLPAGVSMSQFALRWTLMFD
                     AVSCAIPGGKRPEQVAENCLASDLPALSAETMNQVRRIYDEKIRPRVHERW"
     gene            complement(3298..>3981)
                     /locus_tag="VEJ00_03540"
     CDS             complement(3298..>3981)
                     /locus_tag="VEJ00_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00263.1"
                     /translation="DAPTVQVWQAYQQLLTENKFKRLTVKPGDRLPIKGIEATVISSD
                     GAVINHALAGAGQENAKCKGAESFPADQTENRRSLGTLITLGKLRILDLGDLTRDVEM
                     DLVCPNNKLGAMDIYIVSHHGWYQSGSPAFLNAIAPRVAIMDNGAKKGGTPSAWDIIE
                     KSPRLQDLWQLHFSEEGGPGHNVAPEFIANPEGPDAANYLQLTAWPDGSFEIFNSRTS
                     KTKQYSAQH"
BASE COUNT          790 a         1175 c         1191 g          825 t
ORIGIN      
        1 ctgttggtat gatcccgccg gcctggagtg aggacgacgc ttcccagcgt cccgtcgagc
       61 tgcaagtgac tgcggtcagc gctcacactt ttcgcctcag catcctgcca gtgaacaacg
      121 gagaaattgg aacgattcct gccaacggat cgctggtgca ggaatcgtgg ggatcgcctg
      181 agacctttcg caccgcgccc aagggcgagt tcgctgtggc tggttgccgg ctgaccattt
      241 ctcttaaccc agtccgcatc aaaatcgcaa acgagcgagg caacgctatt caacagctcg
      301 attgggacgc gaactcgggc cagctctcgt tcctgagcgg aaaccagcca ctgttcggca
      361 tgggcgaggg cggaccgcaa ttcgaccgcc gcgggtccgt cgaccgcatg aagagcggac
      421 aaggcggcta caagctcgcc acccacggtg gacgtgtgcc cattccatgg atcatcggga
      481 cctcaggatg ggcgatgttc atccatcaac cattcggcac attcgacttc acaggtccgg
      541 tctgcaagtt tcaacctgca agccccgaca gcgctcttcc cctcgacctg ttctttattg
      601 cctctgcgga tccggctacg atcatggccg agtacgctcg catcacgggt aatcctgaaa
      661 aacctccgct atggagcttc ggctatcagc agtcgcaccg cactctggcc agcagggaag
      721 aggtgctggc cgaagctgac acgtttcgcg agaaaaaact cccgtgcgac acgctgatct
      781 acttgggaac gggcttttgt ccttcgggct ggaacagcga gaacggctca ttcgtgtgga
      841 attccagagt ctttcccgat cccaaggatg ttctcgacca gctgcacaag aaaaactttc
      901 gcgccgtcgc gcacgtggtg attctgtccg accgcctgcg cggcacggtt cacgatccgt
      961 gcgacgtttc gcgctttgac gaagaactag cgagctgcca ctgggatcaa catcgtaagg
     1021 actttgccat gggcatcgac ggctggtggc ccgacgaggg cgatccgttg gacatcgcgt
     1081 cgcggctggt tcgcaaccgc atgtactggg aaggtccgca gatagatcgg ccgaacgaac
     1141 gtccttttgc gttgcaccgc aacggatacg ccgggatgca gcgctacgcc tcgttcctct
     1201 ggtcgggcga cgtgtattcc acgtgggaga cgctgaagac gcatctcccg attgctctca
     1261 acaccggact cagcgggatt ccttattggg ggaccgacat cggaggtttt gtccccacgc
     1321 gggagttcac ggcggagttg tacgtccgct ggtttcagtt cggggcgttc tgcccgctgt
     1381 tccgctgcca cgggcgcaca tggaagttgc ggctcccctg gggctggaac acgggtgatc
     1441 ccgggccggt agagattaac aactacaatg gcgccgccat tcccgacccc agccaactgc
     1501 acgacgcccg cgtcgagccc atctgccgga aatatctgga actgcgctat cgcatgttgc
     1561 cctatctcta cagcgcagtg catgagagca cgacgactgg aataccgatc atgcgcgcac
     1621 tgtggctgca ttttcccgac gaccccaaag ccgtgcagtg cgaagatgag tacctgtggg
     1681 gcaagagcgt gctggtcgct ccagttgttg agaaaggagc gacctcgcgc cgcgtctatc
     1741 ttccgccgtc ggcctggtac gatttctgga caggagagcg actcgaaggc gggcgcgaga
     1801 tttcccgccc cgtcgacctt gagacgatgc cgttgtatgt tcgcgcagga tctctcctgc
     1861 cgctaggtcc ggtcaagcaa tacaccggcg aaaaggtcga ggggccgctg tccatttcga
     1921 tctatccggg tgccgatgca tcgttcctgc tctatgagga tgatggaata tcctttagct
     1981 atcgcagcgg agaatggatg ggaatccaga tgtcgtggga tgaaaagcgg cgcatgctga
     2041 gcctgcagct ggccccgggt tcgcgcatgc tgccgcccgg cccgagacca attactgcgc
     2101 aattgggcga aatcaggcga agcgtcgctt tcgagggaaa gccgattgat ctatcgtttt
     2161 agccaccggc atcatacatt ccaggaaagt gagccagcat gaaataccgg ccgctcggca
     2221 gaaccggttg gaatgtttcc gaggtcagct tcggcgcctg ggcgatcggc ggcgcctggg
     2281 ggaaggtttc ggagaaagac gcgctggccg cactccacca ggcggtcgac tcgggcgcaa
     2341 atttcattga cacggctgat gtgtacggca acggccgcag cgagcggctc atcgctcaac
     2401 tcaggaagca acggacggaa acgatcgtag ttgccaccaa ggcaggccgc cggctgccgc
     2461 ggcaaaccgt agaaggttac agtcggaaga atctaaccag ttgggtggaa gacagcctgc
     2521 gcaatctctc tgccgacgcg ctcgacctgc tgcaacttca ctgtccgccg acggagcttt
     2581 actaccagcc cgaggttttc gcaattctcg acgatttcgt gaaggcaggg aagattcgct
     2641 actacggcgt cagcgtcgag aaaatcgagg aggccctcca ggctctccag tggcccaatg
     2701 tccagagcgt gcagatcatc ttcaattgtt ttcgtcagcg gccggcagag gttttcttcg
     2761 cgcgcgcaaa ggaaaaacgg gttggcatcc tggctcgtgt gccgcttgcc agtggcctgc
     2821 tcacgggcaa gcttcaagca acatcgacct ttccggctga cgaccatcgc aacttcaatc
     2881 gccacggaga agcctttgat gttggcgaga cattctctgg cgtggattac gatcttggtc
     2941 tgcaagccgt ggaggaaatt cggcgactct tgccggcggg cgtctccatg agccagttcg
     3001 cactgcgctg gaccctgatg tttgacgcgg tgagctgtgc gatccctggt ggaaagcggc
     3061 ccgagcaggt tgcggaaaac tgcctggcgt cggatctgcc ggcgctatcg gccgaaacca
     3121 tgaaccaggt ccggcgcatt tacgacgaga agatccggcc acgcgttcat gagcggtggt
     3181 gagcaatcaa ggattgccga gatcgaacga tcacgcaacg cgatactcga ccgctattcc
     3241 cgcatgggcc attgcggtgc gcttccaagt gtgtgtgatc actcgaccga cgtggtttta
     3301 gtgctgtgct gaatactgct tggtcttcga cgttcgggaa ttgaagatct cgaaacttcc
     3361 gtccggccag gcggtcaatt gcaaatagtt ggccgcgtcc ggaccttcgg gattggcgat
     3421 gaattctggg gcaacgttgt gcccaggacc gccctcttcg gagaagtgaa gctgccacag
     3481 atcctgcagc cggggagact tctcaatgat gtcccatgcc gaaggagtgc cgcctttttt
     3541 cgcgccgttg tccatgatcg cgacgcgcgg agcgatcgcg ttcaggaatg cagggcttcc
     3601 gctctgatac cagccgtgat gcgaaacgat atagatatcc atcgcgccca gcttgttgtt
     3661 cgggcagacc aggtccattt ccacgtcgcg ggtcagatcg ccgagatcca gaatccgcag
     3721 cttcccgagc gtgatcagcg tcccaaggga tcgccgattc tcagtctggt cggccggaaa
     3781 cgattccgca cctttgcact tagcgttctc ctgcccggcg cctgcaagag catggttgat
     3841 cacggcgcca tcggagctta tgaccgtagc ctcaattccc ttgatgggca gcctgtctcc
     3901 gggcttcacc gtcaggcgct tgaacttgtt ctctgtcagt agctgctggt aggcctgcca
     3961 aacttgaacc gtgggcgcgt c
//
LOCUS       DASPUL010000144         7605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_843370, whole genome shotgun sequence.
ACCESSION   DASPUL010000144 DASPUL010000000
VERSION     DASPUL010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843370"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VEJ00_03545"
     CDS             complement(<1..965)
                     /locus_tag="VEJ00_03545"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HXX00264.1"
                     /translation="MNSAPAYTPRLLPLRLPRVCVAVVGSDPTEMVDKAEVLVRDNSF
                     LELRLDYLSKPGLAMARIKRFTESHPGTVVIATCRRAASGGKFRGSMASQLEILGKAA
                     AAGCQLVDVELQTAAKCRPEQLQKLRNRAAVVLSFHDFRGTKKLDETLQKMLAYPADF
                     YKVVSTATTLADNVTMIKFLAQHGDRHSLVSMCMGEQGIISRVLGVRAGSLFTFASAS
                     AGEETAPGQVTAQELRNVYRIEQVDAATRVYGVAGDPVAHSLSPAIMNAAFRRENVNA
                     VYLALHAKTLKDLLTCVREIPIHGISVTMPYKQPIVPHLDNTDSHT"
     gene            1220..2179
                     /locus_tag="VEJ00_03550"
     CDS             1220..2179
                     /locus_tag="VEJ00_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00265.1"
                     /translation="MKKTGLLVLLLATAAWAQSGADSSATDTPQGTAPTTDSFPSERV
                     QTPTYADLYCAGFINKQILPDANYIAGGLQTPTTTKFTRGDMVYLVGSGYAAGAEYEI
                     IRALRDVNEYEVFPGQTKLLKETGQPYEEVGRVKIIDTRSKTAIARIEYSCDGINPGD
                     TAIPFAEKQSVAFHSPIRFDRFLPGSSKLSGRIVMAKDFDSQLGTGQKVYINVGANQG
                     VKVGDFFRAVRSYDADLKDPVDSLSFRAAMAEDTQKKTPSFDPTLFDYKGNGPTIHVR
                     DLPRRAVGEIVIIGVTPTTATGMIVFSMEDVHAGDGVEADQVN"
     gene            2294..2500
                     /locus_tag="VEJ00_03555"
     CDS             2294..2500
                     /locus_tag="VEJ00_03555"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HXX00266.1"
                     /translation="MATNLAIDDKLIKEARKTGGHKTKKEAVTTALEEYIRYHKQQEI
                     LSDFGTVDFDPAYDYKAARRRKRS"
     gene            2500..2913
                     /locus_tag="VEJ00_03560"
     CDS             2500..2913
                     /locus_tag="VEJ00_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXX00267.1"
                     /translation="MVLVDTPLGSLSLRRRAVDLSLTERGLTRVLYELIQRREVQLLG
                     AIREEVLSGIREESQFQRVRDHLRGFANVPLDSIDYEEAARISNQCRQHGIADSSVDM
                     LLCAVSLRHGWEILTTDRDFVHYAQVIPLRLFSNP"
     gene            complement(2934..3500)
                     /gene="amrA"
                     /locus_tag="VEJ00_03565"
     CDS             complement(2934..3500)
                     /gene="amrA"
                     /locus_tag="VEJ00_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HXX00268.1"
                     /translation="MAVQSGEQSEYSQDERRLLLRLAHESILSGLEQRELSLDPPTVH
                     LAQPRGVFTSLHLHGELRGCVGYVLPVSSLYRAVAETARAAAFEDNRFYPVALDEAGE
                     LEVELSVLSPPQPIRPEEIEIGRHGLLISRGGHRGLLLPQVPVEHHWDRTTFLEQTCH
                     KAGLPADAWQKGATIEAFTAEIFGDTHA"
     gene            complement(3505..4185)
                     /gene="pgsA"
                     /locus_tag="VEJ00_03570"
     CDS             complement(3505..4185)
                     /gene="pgsA"
                     /locus_tag="VEJ00_03570"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HXX00269.1"
                     /translation="MNLPNSITLIRILSIPLLIWILSSPRFSSVNGEKELLASALFIA
                     AAMTDGIDGYLARKRGQITTMGILLDPLADKLLIAAAFITLVQLNPSLVPAWIAVVVI
                     GREFLVSGLRSIAASEGFTIEASELGKFKMVIQIVGVVAVILDHRWKEWPVYGYYVFP
                     IHWIAYLAIWFMVCVSLVSAADYFVAFWSKIDRRVVKRRRRAFILSRRRKPVAAPAQA
                     DRDVATST"
     gene            complement(4324..4737)
                     /locus_tag="VEJ00_03575"
     CDS             complement(4324..4737)
                     /locus_tag="VEJ00_03575"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HXX00270.1"
                     /translation="MGLLDNLENMAMGKVSGSNPEAAAVLEMIENHPGGVNGLVQAFH
                     SNGLGGVVNSWIGTGENQAVTPDQIQQVLGSGPLQAFAQKLGVSPEQAGSTLSQLLPT
                     IMDKLSPNGAVPEHSNLLQMGEGLLASLGKTGTEG"
     gene            complement(4811..5131)
                     /locus_tag="VEJ00_03580"
     CDS             complement(4811..5131)
                     /locus_tag="VEJ00_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXX00271.1"
                     /translation="MIPRNIIRNILVALVVLTLLSSLSVAQASSKSTPKAATAQAASG
                     DLLDINSATKDQLDALPGIGAVYSQKIIDGRPYKAKTDLVKKKIIPQATYNKIKDKII
                     AKQK"
     gene            complement(5154..5378)
                     /locus_tag="VEJ00_03585"
     CDS             complement(5154..5378)
                     /locus_tag="VEJ00_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00272.1"
                     /translation="MAAPAKTSTKSAPAPTQNASDADIAAAKSSGKVWVNLDSKVYHK
                     SGRWYGKTKNGKFMTEDEAKAAGYKASQSN"
     gene            5452..5775
                     /locus_tag="VEJ00_03590"
     CDS             5452..5775
                     /locus_tag="VEJ00_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00273.1"
                     /translation="MEAAAGAAVVFAAGADPESAFAEEPFLLFDDFLLEAVSAAGLAW
                     LLPEAEPAAVAGASVLALFFFDFDDVFVGDDALVAAADVAVELSAAAFFDFLDFLVVE
                     AEVEV"
     gene            complement(6090..7367)
                     /locus_tag="VEJ00_03595"
     CDS             complement(6090..7367)
                     /locus_tag="VEJ00_03595"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HXX00274.1"
                     /translation="MTTMTTAPHVDKETNPWESQRARFDLAAQKLNLDEGLWRVLRYP
                     NREITVHIPVQMDDGHLEVFTGFRVQHSIARGPAKGGIRYAPDVTLDEVRALASWMTW
                     KCAVVNIPFGGAKGGVICDPHKMSIGEVERMTRRYTAELIEFIGPEKDVPAPDVNTNE
                     QVMAWMMDTYSMHMRQTVTAVVTGKPINIGGSRGRREATGRGVMIVCDEACRKLGIDR
                     ARARVIVQGFGNVGSNAALLMHEEGYQVIGIAEVGGGLYNKNGIDVKALVDFRQKNGT
                     VHGFPGAEKYDPAELLITDCEILIPAATENVITTRNVDRVKCRILAEGANGPTTSAAD
                     DVLSDKKVFVIPDILANAGGVTTSYFEWVQDRQGYFWKESVVNEQLETIMKSSFDDVV
                     RYAETHHVNNRIAAYMLAIDRVAYTIRQRGIYA"
BASE COUNT         1515 a         2154 c         2393 g         1543 t
ORIGIN      
        1 gtatgggaat ccgtgttgtc gaggtggggc acgatgggct gcttgtacgg catggtcacg
       61 ctgatgccgt gaatgggaat ctcgcgcacg caggtgagca aatccttcaa ggtcttggca
      121 tgaagtgcaa ggtagacggc attcacgttc tcccggcgaa aggctgcatt cataatggca
      181 ggggacagcg agtgggcgac tggatctcct gccacgccat agacgcgggt ggccgcatcg
      241 acctgctcaa tgcggtagac gttacgcaat tcctgcgccg tcacctggcc gggagcggtt
      301 tcttcgccgg cactggcaga ggcgaaagta aacaagctgc cggcgcgtac tccaagcacg
      361 cggctgatga ttccctgctc gcccatgcac atgctgacca gggagtgacg gtcgccgtgc
      421 tgggccagga acttgatcat ggtgacgttg tcggcgaggg tcgttgccgt cgagacgact
      481 ttgtaaaagt cggcgggata ggccagcatt ttctgcaggg tttcgtcgag ttttttcgtg
      541 ccgcggaagt catggaatga gagtacgacc gcggcccgat tacgcagctt ctgcaattgc
      601 tcggggcggc acttggcggc ggtttgcagc tcgacgtcga ccaactggca accggctgcg
      661 gcggctttgc caaggatctc cagttgcgaa gccatcgagc cccggaactt gcctccactg
      721 gcggcgcgcc ggcaggttgc gatgaccacc gtgcccggat gcgattcggt gaagcgcttg
      781 atccgcgcca tcgccaggcc gggcttcgac agataatcta accttaactc aagaaaggag
      841 ttatcccgaa ccagcacctc ggccttatcg accatttcgg tgggatcgga gccgaccacg
      901 gccacgcaca cccggggcag acgcagcggt agaaggcggg gcgtgtaggc aggggcggag
      961 ttcatcgtgc tacgttcacg ctcggttcaa agctcatcgt ggaattgacg gcgcattatt
     1021 acaggggcgt aattgcagat gcaacaacgg attgatacag ggacgaaaaa ctttttgctg
     1081 ggcgttggcc tacaccctcg taaccttgac ccacccttac ccccttgtta ccttccggct
     1141 gaagcctttg tgtttcaggc atttgaatgc ttttctgact aggggtgggg acccggagcg
     1201 gttctatccc ggaggtaaca tgaagaaaac agggctgtta gtgttgttgc tcgcgaccgc
     1261 ggcatgggcg caatccggtg ccgacagtag cgccacagat actccgcagg ggactgcgcc
     1321 gacgaccgac agtttcccca gtgagcgcgt ccagacgccg acgtatgccg atctctattg
     1381 tgccggattc atcaacaaac agatcctgcc ggatgccaac tacatagcag ggggactgca
     1441 gacgccgacg accaccaagt tcacgcgcgg ggatatggtt tatctggtgg gctccggtta
     1501 tgcagcgggg gccgagtacg aaatcatccg cgcgctgcgc gacgtgaacg agtatgaggt
     1561 gttccccggg cagacgaagc ttctcaagga aacggggcag ccctacgagg aagtcgggcg
     1621 tgtgaagatc atcgacactc gcagcaaaac cgccatcgcc cgaatcgaat actcgtgcga
     1681 cgggatcaac ccaggcgaca cggcgattcc gttcgccgag aagcagtcgg ttgcttttca
     1741 ctcgccgatc cgcttcgacc gcttcctgcc gggcagcagc aagctatccg ggcgcatcgt
     1801 gatggcgaag gatttcgatt cgcagctcgg tacggggcag aaggtgtaca tcaacgtagg
     1861 cgccaaccag ggtgtaaagg tcggcgactt tttccgcgcg gtgcgcagct acgatgcgga
     1921 cctgaaggat ccggtcgatt ctttgtcttt cagagcagcg atggccgagg acacccagaa
     1981 gaaaacgcca tcgttcgatc cgaccctgtt cgactataag ggaaatggcc ccacgattca
     2041 cgtacgcgac ttgccgcggc gcgccgtggg cgagatcgtg atcatcggcg tgacgccgac
     2101 cacggcaacc ggcatgatcg tcttctctat ggaagatgtg catgcgggcg acggcgtgga
     2161 agcagatcaa gtcaactagc tggtttgagg cgagtttgac gaagtcagag accggggacg
     2221 cagaggcgtc accggtcttg tttttgcggg agcgaccagt tgtgttacac actcgatgtg
     2281 taacacttta cacatggcaa caaatctggc aatcgacgac aagctcatca aggaagcgcg
     2341 aaagactggt ggccacaaga cgaagaagga ggcggtcacc acagcgcttg aagaatacat
     2401 ccggtaccac aagcagcagg aaatcctgtc ggatttcggc actgtggact tcgatcccgc
     2461 ctatgactac aaagcggcac gtcgccggaa acgctcttaa tggtattggt cgatactccg
     2521 ctcgggtcgc tttccctccg ccgccgagcc gttgacctct cactcacgga gcgaggtctc
     2581 actcgggtgc tgtatgaact cattcagcga cgtgaggtcc agttgctggg tgctatacgg
     2641 gaagaggtgc tgtcggggat tcgcgaagag tcgcagtttc agcgcgtccg cgaccaccta
     2701 cggggattcg caaacgtgcc gctcgattcc atcgactacg aagaggcggc aagaatcagc
     2761 aatcaatgtc ggcaacatgg gattgcggat tcgtctgtag acatgctttt gtgcgccgta
     2821 tctcttcgcc acgggtggga gattctcacg accgaccgcg acttcgtcca ctacgcgcaa
     2881 gtgattccct tgcgtctgtt ctcaaacccc tgagggaggg actccctcct caatcaggcg
     2941 tgggtatccc cgaagatttc cgcggtaaag gcctcgatgg tcgcgccctt ctgccaggca
     3001 tccgcgggca ggccggcttt gtgacaggtt tgctcgagga aggtagtgcg gtcccagtga
     3061 tgctccacgg gcacctgggg caggagcagt ccccggtgtc cgccgcggct gatgagcagc
     3121 ccgtggcggc cgatttcgat ctcttcgggg cggatgggct gcggaggcga gaggacgctg
     3181 agttccacct caagttcacc agcttcatcc agagccaccg gataaaaccg gttatcttcg
     3241 aatgctgcag cacgggccgt ttcggcgacg gcgcggtaga gcgagctcac cggcagcaca
     3301 tatccgacac atccccgcag ctcgccgtgc agatgcagcg aggtgaatac accgcgcggc
     3361 tgggcgagat gaacggtggg aggatccagc gacagttcgc gctgctcgag gccagagaga
     3421 atggattcgt gggccaggcg gagcagcagt ctgcgctcat cttgcgagta ctcactttgc
     3481 tcgccggact gaactgccac ggggctaggt cgaggtagcg acatcgcggt cggcctgagc
     3541 gggggccgcg acgggtttgc ggcggcggct cagaatgaag gcgcggcggc ggcgcttgac
     3601 cacgcgccga tcaatcttcg accagaaagc gacaaagtag tcggccgccg acaccaggga
     3661 cacgcacacc atgaaccaga tggccaggta tgcgatccag tggatgggaa agacgtagta
     3721 gccgtaaacc ggccattcct tccagcgatg atcgagaatg acggccacca cgcccacgat
     3781 ctgaatgacc atcttgaact tgcccagctc actggcttcg atagtgaagc cctcggacgc
     3841 cgcgatcgag cgcaggccgc tgaccaggaa ttcgcggccg atcacgacaa ctgcaatcca
     3901 ggcgggaacc aggctggggt tcagctgcac cagagttatg aatgcggccg cgatcagcag
     3961 tttgtcagcc aggggatcga gcaggatgcc catcgttgta atctgcccac gcttccgcgc
     4021 gagatagcca tcgatgccgt cggtcatggc ggcggcgatg aacagggccg aagccagcag
     4081 ttctttttcg ccattcacgc tgctgaagcg gggactcgac agaatccaga tcagcagcgg
     4141 gatgctgagg atgcggatga gcgtgatgga gttgggcaga ttcacgctag gggcgggcgc
     4201 cggtgggcca aaccgacaac cagactgatt atcctccacg cacacgggga acgcaatgaa
     4261 cgatcaatac gaggcctctg ggcgggaacg ctagcgcgcc cccgccgcga tccgcgtacc
     4321 tgtctagcct tcggtccccg tcttgccgag cgaggcgaga aggccctctc ccatttgcaa
     4381 caggttggaa tgctcgggga cggcgccgtt gggcgatagt ttgtccatga tggtgggtag
     4441 caattgagac aacgtcgagc ccgcctgctc gggagacacg cccagcttct gggcgaacgc
     4501 ctgcaggggt cccgagccca gcacctgctg aatctgatcc ggcgttacgg cctggttctc
     4561 gccggtgccg atccaggaat tcaccactcc tcccagacca ttgctgtgga aggcctggac
     4621 cagcccattt actccgccgg ggtggttctc gatcatctcc agaaccgcgg ccgcctcagg
     4681 gttggagcca gacacctttc ccattgccat gttttcgaga ttgtcaagaa gacccataaa
     4741 gactccttgc gaaggcttga ttcgaggtta aagaagataa gtgccggcgc aagccgacag
     4801 cttcccagat ttatttttgt ttcgcgatga tcttgtcctt gatcttgttg taggtagcct
     4861 ggggaatgat tttcttcttc accaggtcgg ttttcgcctt gtatggacgg ccatcaataa
     4921 ttttttgcga gtaaaccgcg ccaataccgg gcagggcgtc gagctggtcc ttggtggcgg
     4981 agttgatgtc gagcaagtcg ccggaggcag cctgtgcggt cgccgctttc ggggtcgatt
     5041 tcgaggacgc ttgcgcaacg ctcagcgaac tgagcagtgt gagcacgacc agagctacca
     5101 gaatgttcct gatgatgttc ctgggtatca tgttccctcc tctaagaaat cagctagttg
     5161 gattgagaag ctttgtagcc cgcagccttg gcctcatctt cggtcatgaa cttgccgttc
     5221 ttggtctttc cataccagcg cccgcttttg tggtagacct tgctgtccag attcacccag
     5281 actttacccg aagacttggc ggccgcgatg tcggcatcgg aagcgttctg ggttggcgcc
     5341 ggagccgact tggtcgacgt cttggccggc gcagccatcg tcgaacccga agatgccgcc
     5401 ggggcggcgg ctgccgcggg agctgtggca gagacaggct tcggagccgg agtggaagcg
     5461 gctgcgggcg cggccgtggt ctttgcagcc ggtgcagatc cagagtcagc cttcgccgaa
     5521 gagcccttct tgctctttga cgatttcttg ctggaggctg tttctgcggc cgggctcgcc
     5581 tggctgctgc ccgaggcaga accggctgct gtcgccggcg cctcagtctt ggcgctcttc
     5641 ttcttcgatt tcgacgatgt cttcgtcggg gatgacgcac tcgttgcggc tgctgacgtg
     5701 gccgtcgaac tctcagcggc ggccttcttc gattttttgg atttcttggt ggtggaggca
     5761 gaggtggagg tctgatcgcc ggtggaggtg ctctgggact gcgcccagcc cattgacagg
     5821 cacagcgcca gaaacagaca caacgacccg ccccaagtga ttcgcttagc agttttgctc
     5881 acgggagtct ctcctgatcg agatggtttg gtagcagagc atggttcgca gagcggcgcg
     5941 gttcgatggc agctcagcga gtctaatcag atggaagtag cgcttgaccc gagtacggcc
     6001 caccatgatg ccagccgagt cgaaatcggt caatacacaa aagcagaaag gcctccaccg
     6061 gagtggagga cttcaggaaa ccgtccttct taggcgtaga ttccgcgctg acggatggtg
     6121 taggcgacgc gatcgatcgc tagcatgtac gcggcgatgc ggttgttgac gtggtgagtt
     6181 tctgcatagc ggaccacgtc gtcgaatgac gacttcatga tggtctcgag ctgctcgttg
     6241 actaccgact ccttccagaa gtatccctgg cggtcctgca cccattcgaa ataagaggtg
     6301 gtgacgccgc ccgcattggc cagaatgtcg gggatcacga agactttctt gtccgagaga
     6361 acatcgtcgg ccgcagaggt ggtgggacca ttggcgcctt cggccaaaat gcggcacttg
     6421 acgcgatcga cattgcgggt ggtgatgacg ttttcagtcg cggccgggat gaggatttcg
     6481 caatcggtga tgagtagctc ggccggatcg tacttctcgg cgccggggaa gccatggacg
     6541 gttccgttct tctgccggaa gtcgacaagc gccttcacgt cgatgccgtt tttgttgtac
     6601 aggccgccac ccacctcggc gataccaatc acttggtaac cttcctcatg cataagcagc
     6661 gccgcgttgg agccgacatt gccaaacccc tgcacgatga cacgggcgcg ggcacggtcg
     6721 atgcccagct ttctgcaggc ttcatcgcac acgatcatca cgccgcggcc ggtggcttcg
     6781 cggcggccgc gcgaaccgcc gatgttgatc ggcttaccgg tgactactgc ggtcaccgtc
     6841 tgccgcatgt gcatggaata ggtgtccatc atccaggcca tgacctgttc gttggtgttg
     6901 acgtcggggg cgggaacgtc tttctcagga ccgataaact cgatcagttc ggcggtgtag
     6961 cggcgggtca tgcgctcgac ctcgccgatc gacatcttgt gcggatcgca gatcacgcca
     7021 cctttggcgc cgccgaaagg gatgttgacc acggcgcact tccaggtcat ccagctggcg
     7081 agggcgcgga cttcgtcgag cgtaacgtcg ggggcgtagc ggatgccgcc cttggccggg
     7141 ccgcgggcaa tcgagtgctg cacgcggaaa ccggtaaaga cttcgagatg accgtcatcc
     7201 atctgcacag gaatgtgaac cgtgatctcg cggttgggat agcgcagaac ccgccaaagg
     7261 ccttcatcga gattgagctt ctgggcagcc agatcgaagc gggcgcgctg cgactcccac
     7321 gggttggttt ctttgtccac gtgcggggcg gtggtcatcg ttgtcataag aaattctccg
     7381 gaatgaatgc aggtggtggc cgcagaccgc gaatctagcg gcgggcaaac gttccgagta
     7441 tatgagggga aatggccggg aagcaagcag gagcgggtcg cacgagccgc tcagggcggg
     7501 gtcacgcttt cattgacttg gccttcacag caacgtaaga ttctgctccg atgatcggac
     7561 agacgatctc ccactaccgc atccttgaaa aactgggcgg cgggg
//
LOCUS       DASPUL010000145         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_298414, whole genome shotgun sequence.
ACCESSION   DASPUL010000145 DASPUL010000000
VERSION     DASPUL010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298414"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(563..1951)
                     /locus_tag="VEJ00_03600"
     CDS             complement(563..1951)
                     /locus_tag="VEJ00_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXX00275.1"
                     /translation="MVAFVLLRVFLILLLVAANAFFAAAEFALVSVRDTRIQQLIEAG
                     RIGARIVQRLHQHLDEVVNGVQLGITMVSLTLGWIGEAMVARLVESMQFVHRVPHAAI
                     YAHTIAIVIAFSLITYMHVILGELVPKTLALQRAEQIALAVAAPMEAFLTITRPILFF
                     MRRSGGLVLRLFGAQPTRRGGAVHSPDELKLIVTASRQFGQIPEFQEEMIHSAIELDS
                     ITVREVMVARPDIFSLPSDLTLDDALARVVEGQHSRIPVYDPQRGPEHIVGVLYYKDL
                     MRWMRLRLSNLGQTSSIRITRMQIGQIMHDVLVVPETKVLADLLAEFKTRRRHLAVVV
                     DEFGSTAGVITVEDILEQLVGNIEDEFDVAPSEPALEDESVLLLEGAVNIRDLEAEYG
                     LTLPRDAGFETLAGFILARLQRIPKLRDHFEYEGRRYTVEEIEGHRIARVRIEKTAKV
                     EGEAEAAKQSGD"
BASE COUNT          465 a          652 c          779 g          425 t
ORIGIN      
        1 ccttgccaat agcgcaggta ttgctcgccg agcggggcat cgagaccgaa ggcgtggcgc
       61 gtggcggcga tgtcggcggg cggcgcgccc tcgccgagca tctgctggat ggggtctccg
      121 gggacgaggt ggatgaggag gaagacgagg gagaccacga gccagatgac gggcacggtg
      181 tagaggacgc gttggaggat ggaagtcagc ggtcagtgtc cagtggtcgg catgcaggtt
      241 ctcatcggat tgtcatcctg agcggagcgg gcgcgaatgc gcccgcggag tcgaaggatc
      301 cctataagca ccacgctgcg tcgtttgggt atccgtcgac tgcggctcgc aaaacaaacc
      361 gcaacacttt gctcagggtg agcgctccct agaacgccac ccgtttcctt cagcggctaa
      421 agccgcccgg tagaatgccc catacgcggc cctaaagggc cgctcttcca cggagcggta
      481 aggtccggta cgaacccgcg cgacgagtgg cgcccttccc gttcgtacga tcggtgcggc
      541 tgaaagccgc acctttcaaa ccttagtccc cgctttgctt cgccgcctcg gcctcgcctt
      601 cgaccttcgc tgttttctcg atgcgcacac gggcgatgcg gtggccctct atctcttcga
      661 cggtgtagcg gcggccttcg tattcgaagt ggtcgcgcag cttggggatg cgttgcaggc
      721 gcgccagaat aaagccggcc agagtctcaa agccggcgtc tcggggcaac gtcaagccgt
      781 attctgcttc caggtctcga atgttcacgg cgccttccag cagcagcacg gattcgtcct
      841 cgagagcagg ctcgctgggc gccacatcga actcgtcttc gatgttgccg accagttgct
      901 cgaggatgtc ctcgacggtg atgacgccgg cggttgagcc gaattcgtcg accacaaccg
      961 cgagatgtcg gcggcgcgtc ttaaattccg caagcaagtc ggccagcacc ttggtttcgg
     1021 gcacgaccag cacgtcgtgc atgatctggc cgatctgcat gcgggtgatg cggatcgagc
     1081 tggtctggcc gagattgctc aggcggaggc gcatccagcg catgaggtcc ttgtagtaga
     1141 gcacgccgac gatgtgctcc ggaccgcgct gcggatcata gactggaatg cgagagtgct
     1201 ggccttcgac cacgcgggcg agggcgtcgt caagcgtgag gtcggagggc aaagaaaaaa
     1261 tgtcaggccg ggcgaccatg acttcgcgca cggtaatgct gtcgagttcg atagcgctgt
     1321 ggatcatttc ttcctgaaat tcgggaatct gcccgaactg ccggctcgcg gtgacgatca
     1381 gcttgagttc gtcgggggaa tgaacggcac cgccgcggcg cgtgggctgg gcgccgaaga
     1441 ggcgcaggac gaggccgccg gagcggcgca tgaagaagag aatcgggcgc gtgattgtaa
     1501 ggaaggcttc catgggcgcg gcgacggcca aggcgatctg ctccgcgcgc tgcagcgcca
     1561 gagtcttggg cacgagctcg cccagaatca cgtgcatata cgtgataagg ctgaaggcga
     1621 tgacgatagc gatggtgtgg gcgtagatgg cggcgtgcgg cacgcggtgc acgaattgca
     1681 tgctctcgac gagacgggcc accatggcct cgccgatcca gcccagggtg aggctgacca
     1741 tggtgatgcc gagctgaacg ccgttgacga cttcatcgag atgctggtgc aaccgctgga
     1801 caatgcgggc gccaatgcgt ccggcttcga tgagctgctg gatgcgggtg tcgcgcacgc
     1861 tgaccagggc gaactcggcg gcggcgaaga aggcattggc cgctaccagg agaaggatca
     1921 ggaagacccg gagcagcaca aaggcgacca tggaagggta attctaacat ttggaagcag
     1981 ttgcgcggtt ggtgaccagt tgtcggtgat cagtggtcag catcaggcgg agggtgctcg
     2041 attcggaacg aacccttctt cactggatga aaattccatt ctcaccgcag ctatcctttt
     2101 cggaggacct ggggtctaac tctcaggcgg aggcggattt ggcaaccgca cgtcttcttt
     2161 tgcctcttag ctcaggaccg cacgtctcaa aaaagcagga actaagacaa agctgaccgc
     2221 gtacataaga gtgagggcgc aatggctcct catctcagcc tggcaaatcc ccaaggaggc
     2281 gttgtgaacg ggaacggcaa tcataagaag aaacgccgca g
//
LOCUS       DASPUL010000146         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1939927, whole genome shotgun sequence.
ACCESSION   DASPUL010000146 DASPUL010000000
VERSION     DASPUL010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1939927"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(503..1900)
                     /gene="hydA"
                     /locus_tag="VEJ00_03605"
     CDS             complement(503..1900)
                     /gene="hydA"
                     /locus_tag="VEJ00_03605"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HXX00276.1"
                     /translation="MAFDTIIRNGSIITAADTYTADVAITSGKIAAIGTDLPTQNASK
                     VLDAKGKLVLPGGIDVHTHLDMPFGGTTSADDFETGTRAAAFGGTTTLIDFAIQYKGQ
                     ALRQAFDTWMGKAANKAVCDYAFHCIVTDVSGGQLGEMNDLVREGVTSFKLFMAYPGV
                     FMLDDASIFKALQRTSKNGSLICMHAENGSAIDVIVQQALAEGKKAPKYHALTRPTTA
                     EAEATARAIALAEMAGAPIYIVHLSCNDALEKVREARDRGLPVYAETCPQYLYLSIEN
                     FDVPGFEGAKYVFTPPLREKWHQEKLWQGLQRDHLQVVSTDHCPFCFKEQKELGKDDF
                     TKIPNGGPGVEHRMSLIYSGGVAQGRFSANRFVELVSTTPARLFGLYPRKGTIAVGSD
                     ADLVVFDPKRRHSISAKTHHMRVDYSMFEGIEVTGMPDVVLSRGRVVVEGDKFVGRAG
                     QGEFLRRATYAQVNG"
BASE COUNT          504 a          760 c          699 g          536 t
ORIGIN      
        1 taaaaaagtt cgaagcgata gttctttacg cccttgtagg tattggccca cgacccccag
       61 cagatggcgc tggtgatcat catcagcaac gctatggtca aagtcggcgg cgtgaacatg
      121 gtccctccca caggagtcgc acaggaaagt gtgtaactct actctcgttg gcaccgagcg
      181 acaagatcag gagttagctg tccgaatttt tccctgccga gtggacaacc atttcgacca
      241 ttgcaccagc cttggtgcta ccattcgtaa ggcaaagaat cgcggcccgt tagaaggatc
      301 gggcctgtgg aagagattcc tgctaagcga ttgatgacaa aaggcttaga tccagcaatt
      361 aaatcgattt agaaagccgc tgcaggcccc cgaaaagcgg ccgctctgaa cactcacctt
      421 gcgcagccgc acgtgttgga tgctgtcaga atcgcagcgc ggggcatctc atgacgcgac
      481 cggctcgcag cagacgcaac tctcaaccat tcacttgcgc gtaggtagca cgccgcagaa
      541 attctccctg cccggctcgc cccacgaact tatcgccttc cacgacaact cgtcctcgcg
      601 aaaggacgac gtcaggcatg ccggtgacct cgatgccttc gaacatcgag tagtcgaccc
      661 gcatgtggtg cgttttggca ctgatgctgt gcctgcgctt ggggtcgaag acgacgaggt
      721 cggcgtcgct gccgacggcg atcgtccctt tgcgcggata caggccgaac aatctcgcgg
      781 gagtggtcga gaccaattcc acaaaacgat tggcgctgaa acgtccttga gccacgccgc
      841 cggaatagat caggctcatg cgatgctcca caccagggcc gccgttgggg atcttggtga
      901 agtcgtcttt gccgagttct ttctgctctt tgaagcagaa cgggcagtgg tcggtcgaga
      961 ccacctgcaa atgatcgcgc tgcaggcctt gccataactt ctcctgatgc cacttttcgc
     1021 gcagcggcgg cgtgaatacg tacttcgcac cttcaaagcc gggcacatcg aagttctcga
     1081 tcgacaaata gagatactgc ggacaagtct cggcgtaaac cggcaggccg cggtcgcgcg
     1141 cctcgcgcac tttttcgagc gcgtcgttgc agctcaggtg cacgatgtaa attggcgcgc
     1201 ccgccatctc cgccagcgca atagcgcgag cggtggcttc ggcctcggcg gtcgtggggc
     1261 gcgtaagcgc gtggtacttc ggcgccttct ttccttccgc cagcgcctgc tgcacgatca
     1321 cgtcgatggc gctgccgttt tcggcgtgca tgcagatcag gctgccattc ttcgacgttc
     1381 tctgcagcgc tttgaaaatg ctcgcgtcat cgagcatgaa cacgccggga tacgccatga
     1441 acagcttgaa gctggttacg ccttctcgca ccagatcgtt catctcaccg agctgcccgc
     1501 ccgacacgtc ggtgacgatg cagtgaaacg cgtagtcgca aacggctttg ttcgcagctt
     1561 tgcccatcca cgtgtcgaag gcctggcgca gcgcctggcc tttgtattga atggcgaagt
     1621 cgatgagtgt ggtcgtcccg ccgaaggcgg cggcgcgcgt gccggtttca aaatcgtcgg
     1681 cgctggtggt gccgccgaag ggcatgtcga gatgcgtgtg cacgtcgatg ccgccgggca
     1741 gcacaagctt tcccttcgcg tcgagtacct tgctcgcgtt ttgcgtgggc agatcggttc
     1801 caatcgctgc gatcttgccg ctggtgatcg ccacatcagc ggtgtaagtg tcagctgcag
     1861 tgatgatgga accgttgcgg atgatggtat cgaaagccat gcgcttctcc tgtgatttcc
     1921 ctcgcgtcct tcgcgcaccc atggcgacct ttgcggtaac acttcttgcc ccgcgaagtt
     1981 cgcaagaatc gcgaagggac gcgaccctat gctcgcgaac ggtctatgtt gacgctttca
     2041 gtcatctttc ccataaagat gggtcacgac gagtgacgct accagccccg ccactaagag
     2101 cccggccgtc ctcacgcccg gattcccgaa caaaagaaca atgacacagc atccggcttc
     2161 caggattatg gctgatttgt gtttcatctc gctctcgcct cactccctgc ccaaacggcc
     2221 caacaacgga caggtgtcag acctcagaat cgcgcgcccg gaatgctcag gtccaacggg
     2281 aaaagaattc tatgctacac tgccgctcct cggcgctcat ttgttctctg agcaggaagc
     2341 cctaatgcat ttcggcatca ccatcaagcc cgatatctcc atcgaccgca tcgtcggtct
     2401 gacgcggcaa gccgaggccg caggcttcga atacagctgg atattcgatt cccacgtttt
     2461 gtggatggag ccctacccgc tgatgacgct catcgcggc
//
LOCUS       DASPUL010000147         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1392174, whole genome shotgun sequence.
ACCESSION   DASPUL010000147 DASPUL010000000
VERSION     DASPUL010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392174"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VEJ00_03610"
     CDS             complement(<1..1427)
                     /locus_tag="VEJ00_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXX00277.1"
                     /translation="MTTNIVSQEKGKPVFDEQALAKLLESAYVLQEHNRKMQKPVPPH
                     ETPAPLPRRQERPIHAAPAAPEVSPQERASGRDYTFILAQIVDAQRQIQVDHLGLEDA
                     MSKVASLLVEMTKAAGASIGIVEGQQIRYRTAIGHGALAVGAVMAAENTLSAECLRTG
                     QVVRCTNVDTESAFYAGECRQRGIFSLIAVPVYHDTAVEGSLELYFAHPDGFTEQDVH
                     TCQLMSGLVTEALTRDNELNWKKSLASERAAMLEALEKLKPNLAALRNTQALARESAA
                     HAAAPAPKSAEPAAVCARCGHPLVADELFCGKCGSPRSADHEPATTMQSKVATLMNLP
                     PSRKGAASARASAAVEMAPVDALQPEEPVWDEARGSDSPAAAALREHVPASADTSDAS
                     IADLEIEFQPEDDREQDASPETDTATTVPAASSLTPSSVKPVPAADWSSAAAAREFLE
                     QLEPVKRPDALSRFWKARRGDIYLAV"
     gene            complement(1731..>2190)
                     /locus_tag="VEJ00_03615"
     CDS             complement(1731..>2190)
                     /locus_tag="VEJ00_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXX00278.1"
                     /translation="FGSFAAPVAILSSALLSTSGVFIALLLTRVTFNISSFMGMIMVI
                     GIVAKNGILLLDADQKMRALGLSAEDAMLQAARRRLRPIVMTALATVAGMLPLAFALG
                     AGSQMLQPLAIAVIGGVLISMVLSLIITPAVHFYLAGRDEPVTEVLPRVS"
BASE COUNT          418 a          661 c          692 g          419 t
ORIGIN      
        1 actgcgaggt agatatcccc tcgccgggct ttccagaaac ggctcagagc gtccgggcgt
       61 ttgaccggct ccagttgttc gaggaattcg cgggcggccg cagccgaact ccaatcggca
      121 gccggcacgg gcttgacgga actcggtgtg agtgaagacg ccgccggcac ggtagttgcg
      181 gtatcggtct cgggcgaggc atcctgctca cggtcatctt caggctggaa ttcaatttcc
      241 agatctgcaa tcgaggcgtc tgaggtatcg gccgacgcag gaacgtgctc tcgaagcgct
      301 gccgccgcag gagaatccga gcctctcgcc tcgtcccaaa cgggctcttc aggttgcaga
      361 gcgtcaacgg gcgccatttc cacggctgcc gaagccctcg ctgaagccgc tccctttctt
      421 gacgggggca gattcatcag cgtggcgacc ttgctctgca tcgtcgtagc tggctcgtgg
      481 tcggcgctgc gaggcgatcc gcactttcca cagaagagtt cgtctgctac cagcggatga
      541 ccacagcggg cacagaccgc tgcaggctcc gcggatttcg gcgccggagc cgctgcatga
      601 gccgccgatt ccctggctaa cgcttgcgta ttccgcaacg ctgccaggtt gggtttcagc
      661 ttctccagcg cttcgagcat ggcggcgcgc tcgctggcga gcgacttctt ccagttcagt
      721 tcgttgtcgc gcgtgagcgc ctcggtcacc aacccgctca tgagttggca ggtatgaaca
      781 tcctgttcgg taaagccgtc agggtgcgcg aagtagagtt ccaggcttcc ctcgactgca
      841 gtgtcgtgat agacgggcac ggcgatcagc gagaaaatcc cgcgctggcg gcattcgccg
      901 gcatagaacg ccgactcggt gtcgacattg gtgcagcgca cgacctgacc tgtgcgcagg
      961 cactcggcgg acagcgtgtt ttccgccgcc atgaccgctc ccactgctaa ggcaccgtgt
     1021 ccgatggccg tgcggtagcg gatctgctgg ccctcgacaa tgccgataga agcgcctgct
     1081 gccttggtca tttcgaccag caggcttgcc accttcgaca tcgcgtcttc cagtcccaga
     1141 tgatcgacct gaatctgacg ctgggcgtcg acgatttgag ccaggatgaa ggtgtaatct
     1201 cgcccggatg cccgctcttg cggggaaact tccggcgccg cgggggcggc gtgaatgggc
     1261 cgttcctgcc gccgtggcag cggtgcagga gtctcgtgcg gcggcaccgg cttctgcatc
     1321 ttgcggttgt gctcctgcaa gacataggcg gactccagca gcttcgccag ggcctgctca
     1381 tcaaagaccg gtttgccttt ttcttgggac acgatgtttg tcgtcatggg gctgaagaaa
     1441 ctgcccgatc gacgccgctc cagactccgc aatgttccta ttctagagca agttccacgc
     1501 cacaaaagcg ggtgagtgcc gttccgaacg gagaaggata cgcattctcg agctaccttc
     1561 gcttcttggt aaggaagaca gccgctggtt ttccaatagc ttacaagcgg aagtaattgt
     1621 cggggcattc ttaccgcgac actggcgcaa ggcaggcagc gaatcctgaa gtagcggtta
     1681 ccggagtaac gcgcagaact gtgcacactg ttcccacgca gttccctttt tcaagacacc
     1741 ctaggcaaca cctcggtgac cggttcatct ctgcccgcga gatagaagtg gacggcggga
     1801 gtgatgatga gcgagagcac catcgagatc agaacgccgc cgatcacggc aatggccagc
     1861 ggttgcagca tctgcgagcc cgctcccagg gcaaacgcca gcggaagcat gccggccacg
     1921 gtcgcgagcg cggtcatgac gatggggcgc aagcggcggc gcgcggcctg cagcatggcg
     1981 tcctcagcgg acaggcccag ggcgcgcatc ttctgatcgg cgtcgagcaa caggatgccg
     2041 tttttggcaa cgatgccgat gaccatgatc atccccatga atgacgaaat gttgaaggtg
     2101 acgcgcgtga gcagcagggc gatgaacacg ccggaagtgg agagcagcgc cgaggagaga
     2161 atggcgacag gcgcggcgaa agatccgaac
//
LOCUS       DASPUL010000148         2760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_575090, whole genome shotgun sequence.
ACCESSION   DASPUL010000148 DASPUL010000000
VERSION     DASPUL010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575090"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VEJ00_03620"
     CDS             complement(<1..330)
                     /locus_tag="VEJ00_03620"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HXX00279.1"
                     /translation="MATSVVARPGRASRSGVALGVFLLLLLALAGKFIVHYAVPYFAF
                     NPAYFDYYWPHRFRLITHICGGILALLCGPFQFWTGLRNRALNIHRWTGILYLVGVAV
                     GASGAGMM"
     gene            complement(448..1098)
                     /locus_tag="VEJ00_03625"
     CDS             complement(448..1098)
                     /locus_tag="VEJ00_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXX00280.1"
                     /translation="MPTSRTRVVREAHRGVYDRETVYRILDEGFLCHVGFLLDGQPFV
                     IPTSYGRKDGSLFIHGSAASRMLKQMKEGIPVCVTVTLLDGLVLARSVFNHSMNYRSV
                     VILGKATLVEEPNEKLAALRVLSEHILPGRWDDSRQPNERELKQTSVLRVPIEEFSAK
                     VRTGPPTDDEEDYSFPTWAGVLPLEMKAGEPIRDPKLDPIRAVPPYVQGYSRQRSS"
     gene            complement(1257..>2760)
                     /locus_tag="VEJ00_03630"
     CDS             complement(1257..>2760)
                     /locus_tag="VEJ00_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00281.1"
                     /translation="SGAGQPGDLNSFAIFQGVPLGVPGNPCSPATSAATATAMDYSPS
                     QQRFSCAASGTSCGVTSQSIFINQGYLNPSTFLPVAFQPFGYPQSKNFVYAYAQQANF
                     SLERDLGGGFALNLAYNFNGGRHLNRPINANAIRGDLMTANFFAAVADATAAGTAVPA
                     NPLFVTGCSPGGSPTSPLPFVDSSLMGFFRPSGLNPSIIGAYQAIHDPQGCIGQAVSL
                     VHTLPGIDPNCDPATFNGCIPLGDMDANYSNGSSVYHGFSANLRKRFNNHYEFLASYT
                     WSHAIDDSTDLQATLTPQDSFYPALDRSSSLFDQRNRFVFSGVYQTGKLGSHGFAGKV
                     FSDWTFAPVIEVSSGRPYNIITGNGDNLQLSSLTGRPNTFVNPACTAQGNQPLNSKAS
                     PTGVFQEPCISGFTGTLLSLDGNLGRNAGITPWTLFDDMRVSKRINFSERINMDLIAD
                     MFNIANRYNVAAVTPQCCTANPANPTQSTTGQATAAWDPRQFQFAMKVNW"
BASE COUNT          586 a          756 c          915 g          503 t
ORIGIN      
        1 catcatgccg gcgccgctcg cgcccacagc gactccaacc aagtagagaa tgccggtcca
       61 gcgatggatg ttgagagcgc gatttctgag cccggtccag aactgaaatg gcccgcagag
      121 gagcgccaga atgcctccgc aaatatgggt gatcagacgg aagcggtgcg gccagtagta
      181 gtcgaaatag gcggggttga aagcaaaata gggaaccgca taatggacga tgaatttgcc
      241 cgccagcgcc agaagcagca gcaggaatac gcccagcgca actcccgagc gagatgcccg
      301 gccgggacgt gccacaactg aagttgccac gttgcaagct ccttttcgtg cctatgttgt
      361 ggaagacagg accccgacaa atgtcgagag tctgccccag cgagaacaca ttgtcaagga
      421 gcgttgcggg caagatcccg ggagaaatca ggatgaccgc tgacgcgaat aaccttgaac
      481 gtacggcgga acggcgcgga tcggatcgag tttaggatcg cggatcggct cgcctgcctt
      541 catctccagc ggcaggaccc ctgcccatgt cgggaaggag tagtcttcct cgtcgtcggt
      601 cggcgggccg gtgcgcactt tggccgagaa ctcctcgatc ggcacgcgca gcacggacgt
      661 ttgcttgagt tcgcgttcgt tgggctggcg cgagtcatcc cagcggccgg gcagaatgtg
      721 ttccgagagc acccgcagcg ccgccagttt ctcgttcggc tcctccacca gcgtggcctt
      781 ccccagaatc acgaccgagc ggtagttcat ggaatgattg aacaccgagc gcgccaatac
      841 caacccgtcg agcaacgtca cggtcacgca aaccggtatg ccctccttca tctgcttcag
      901 catgcggctg gcggctgagc cgtgaatgaa caagctccca tccttgcggc cgtacgaggt
      961 cggaatcacg aagggttggc cgtcgagcaa aaaaccgacg tggcagagga agccttcgtc
     1021 gagaatgcga tagaccgtct cgcggtcgta aacgccgcga tgggcttcgc ggaccacacg
     1081 cgtccgcgaa gtgggcattt ccgtttctgg cattcaggag gaactccggg caagcgccaa
     1141 ggatgggtaa aaacagagtg taacacttcg gacctcgggc gtcggacctc agccctgccg
     1201 cagtgtggag tctgcagtct gaggtctgag gtccccggtc tgaggtcggg cagtctctac
     1261 cagttcacct tcatcgcgaa ctggaactgg cgcgggtccc aggccgccgt ggcctgtccg
     1321 gttgtggatt gagtggggtt ggcggggttg gcggtgcagc actgcggggt gaccgcagcc
     1381 acgttgtacc ggttggcaat gttgaacata tccgcgatca aatccatgtt gatgcgctcg
     1441 ctgaagttga tgcgcttgga gacccgcatg tcgtcgaaca acgtccaggg agtgatcccg
     1501 gcattgcggc ccaggttgcc atcgagcgaa agcagagtac cggtgaagcc tgaaatgcaa
     1561 ggctcctgga agacgcccgt tggcgatgcc ttcgagttga gcggctggtt gccctgcgct
     1621 gtgcatgcgg gattcacgaa cgtattggga cgcccggtaa gcgaggacaa ctgcaggttg
     1681 tcgccgttac ccgtgatgat attgtacggt ctgccggagc taacttcgat gaccggagcg
     1741 aacgtccagt cgctgaacac ctttccggca aagccgtggc tgccaagctt acccgtctga
     1801 taaacgccgc tgaagacaaa acgattccgc tgatcaaaca aggagctgga gcggtccaga
     1861 gccgggtaaa aactgtcctg cggcgtgagt gtggcctgca ggtcggtgga gtcgtcaatg
     1921 gcgtgcgacc aggtgtagga agcgaggaat tcgtagtggt tgttgaagcg cttgcgcagg
     1981 ttggcactga agccgtggta gaccgaactg ccgttggagt agttggcatc catgtcgccg
     2041 agggggatgc atccgttgaa tgtcgccgga tcgcagttcg ggtctatgcc ggggagagtg
     2101 tgaaccaggc tcaccgcctg cccgatgcag ccttgcggat cgtgaatggc ttggtatgcg
     2161 ccaatgatgg acggattcaa gccgctgggc cggaagaagc ccatcagcga cgaatcgacg
     2221 aatgggagtg gcgacgttgg cgagccgcca gggctgcatc cggtcacaaa tagagggttg
     2281 gcgggcacag ctgtccccgc cgcagttgca tcggccaccg cagcgaagaa attggcggtc
     2341 atcaaatcgc cgcgaatggc gttggcgtta atggggcgat tcagatgccg gccaccgttg
     2401 aagttatagg ccaggttgag cgcgaagcca ccgccgagat cccgctccag actgaagttt
     2461 gcctgttggg catacgcgta gacgaaattc ttcgactgcg gataaccgaa gggttggaag
     2521 gccaccggca ggaaggtgga gggattgaga taaccctggt ttataaaaat cgactgggaa
     2581 gtaacgccgc acgaggtgcc ggaagcggcg cacgagaagc gttgctggga cggcgagtag
     2641 tccatggcgg tcgccgtggc cgcagaggtc gcgggcgagc acggattgcc ggggacaccg
     2701 agcggcacgc cctggaagat tgcgaaggag ttcaaatcgc cgggttggcc cgccccggag
//
LOCUS       DASPUL010000149         5208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_707105, whole genome shotgun sequence.
ACCESSION   DASPUL010000149 DASPUL010000000
VERSION     DASPUL010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707105"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VEJ00_03635"
     CDS             <1..537
                     /locus_tag="VEJ00_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HXX00282.1"
                     /translation="TPNAIQSLRKSGLHVVMLTGDQRVTAERIAKPLGIDEFEAEVLP
                     EQKSEVIKKLQGQGRVVAMAGDGINDAPALAQADVGIAMGTGTDVAMESGGITLVKGD
                     LTGIVRARKLSQATMRNIRQNLFFAFIYNAIGVPIAAGVLYPFFGLLLSPIFAAAAMS
                     FSSVSVITNALRLRHVKL"
     gene            complement(541..1278)
                     /locus_tag="VEJ00_03640"
     CDS             complement(541..1278)
                     /locus_tag="VEJ00_03640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX00283.1"
                     /translation="MPSRRAQLLPLQGKAALVTGATRGIGLAIAHALAAEGCDLILAG
                     RSEAGLRRINREFGRAKAGLLVQGCDVRDPHSVDDLFRQIRRQFQRLDILINNAGIAH
                     PNLPVEKLPFPVWRDVLETNLDGMFLVTQAALVMMKRGSTVVNNLSIAANRVFAGSAA
                     YNASKHGALGLTNTLREELRPRGIRVIGLLPGATDTDIWKTLWPAAPRRKMMSADTVA
                     RAVVQAILLPENATVETLEILPTAGTL"
     gene            complement(1286..1810)
                     /locus_tag="VEJ00_03645"
     CDS             complement(1286..1810)
                     /locus_tag="VEJ00_03645"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXX00284.1"
                     /translation="MIASVFIGTSVDGFIARPNGDLDFLPPDGGEPHGYNEFFASVDA
                     IVTGRGTFETVLAYPAWPYGKKPVVVLSSRPLDFSSVRDGVVEQMSGSPTEIVGKLAA
                     RGWQHIYVDGGVTIQRFLRQGLIQRLIITRVPVLIGGGIPLFGELPRDVQLRHVETTA
                     YASGLVKTEYQIVG"
     gene            complement(1877..2476)
                     /gene="folE"
                     /locus_tag="VEJ00_03650"
     CDS             complement(1877..2476)
                     /gene="folE"
                     /locus_tag="VEJ00_03650"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HXX00285.1"
                     /translation="MKPAIEPATLTSASFEELVREMLIRLGEDPEREGLTSTPKRVRK
                     AMQYLTRGYNEDPEALLKGALFTVTYDEMVIVKDVEMFSLCEHHMLPFFGKVHVAYIP
                     NGRVIGLSKIPRLIEVFSRRLQIQERLTTQIAETIQKVIEPQGVGVVIEARHLCMMMR
                     GVEKQHSAAVTSSMLGCFRNEEETRSEFLSLIRQRANGV"
     gene            complement(2532..3044)
                     /locus_tag="VEJ00_03655"
     CDS             complement(2532..3044)
                     /locus_tag="VEJ00_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HXX00286.1"
                     /translation="MRRPICLSTSTERSEHKSEDIEARGCEFQNMKAHLTRRYRFSAS
                     HRLNSAEMSADENRATYGKCNNPHGHGHNYALEVTVSGRVDDRTGMVCNLADLDGCVE
                     REIVSRYDLENLNTLEEFTRSVPTTENLCIEIFEILQRDFHLAHLERVRLDETMMNSF
                     EYAGGDEPKR"
     gene            complement(3001..3411)
                     /locus_tag="VEJ00_03660"
     CDS             complement(3001..3411)
                     /locus_tag="VEJ00_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HXX00287.1"
                     /translation="MVYLTRKAEFSASHYYHNPEFTAEENRRVFGKCNNPNGHGHNYT
                     LEVTVKGEVDPRSGFVVDLKQLKETMHGEVLDAMDHRFLNKEVPEFATRIPTTENLAI
                     AIWERLEPKLQKARLHRVRVYETPDLFVDFYGEE"
     gene            complement(3432..4484)
                     /locus_tag="VEJ00_03665"
     CDS             complement(3432..4484)
                     /locus_tag="VEJ00_03665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXX00288.1"
                     /translation="MAATRTGTLQTAAEPVVSVIVPARNEESCLGMCLESLLRQTEVP
                     FEVIVVNDHSADRTREIAASFADVRVIEAGPLPDGWTGKNNAVTCGARLARGEWLLFT
                     DADTVHLPGSLGRAVTEAKENGLELLSYSPEQIAVGFWEMATLPVIFADLARQYPPAR
                     VSDPASPIAAANGQYILIRRDAYDAIGGHVAIAEEILEDVALARAVKSSGRKIRFRYA
                     DDAVRTRMYRSYRQLREGWTKNLALLFPGPGWLAVKTLALWAVPWTALFLGLRHPIRP
                     SWLWSAIFAGSFVQLVIRIKRANFGFIMNLLGVAVGMPMFAYLLLQSQRAHAKGKVGW
                     KGRTYSDKSSLLATGH"
     gene            4510..5004
                     /locus_tag="VEJ00_03670"
     CDS             4510..5004
                     /locus_tag="VEJ00_03670"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXX00289.1"
                     /translation="MRRLASFISLFVLLVSLSACYSGTRPPRIGSTAPDFTVQDADAK
                     VTLTQFRGQVVVLNFWATWCPPCIEEMPSLVEMQRRMKAKGVTVVAISIDVDDNAYRR
                     FIKDHAANLLTVRDPDQKTANLYGTHGWPETYIIDRNGVMRRKFIGPVDWTDPEITNF
                     LNKL"
BASE COUNT         1046 a         1663 c         1427 g         1072 t
ORIGIN      
        1 actccaaatg caattcagag cctccgcaaa agcggtttgc atgtcgtgat gctgactggg
       61 gaccagcgcg tgaccgccga gagaatcgcg aaaccgctgg gcatcgacga attcgaagcc
      121 gaggtcctac ccgaacaaaa atctgaagtg atcaagaaac tgcaggggca gggcagagtc
      181 gtcgccatgg ccggcgacgg gatcaacgac gcccccgcgc tcgctcaagc ggacgtcggc
      241 atcgccatgg gcactggcac tgacgtcgcc atggaaagcg gcggcattac gctggtgaaa
      301 ggcgatctga ccggaatcgt ccgtgcccgc aagctgagcc aggccactat gcgcaacatc
      361 cggcaaaatc tcttcttcgc ctttatctac aacgcgatcg gtgtcccgat cgcggccggc
      421 gtgctttatc catttttcgg cctgttgctg agtcccatct tcgccgctgc ggccatgagc
      481 ttcagttccg tctcagtaat cacgaacgcc ctgcgcctgc ggcatgtgaa gctgtaagcg
      541 ctaaagcgtc cccgccgtcg gcaaaatctc cagggtctca accgttgcat tctccggcag
      601 caaaattgcc tgcacgactg cgcgtgccac cgtgtcggcc gacatcatct tgcgccgggg
      661 cgccgcgggc cacaaagtct tccatatgtc agtgtccgtc gcgcccggca gcagcccgat
      721 cacccgaatc cccctgggcc gcaactcctc gcgcagggtg ttggtcaggc ccagcgctcc
      781 gtgctttgac gcgttgtacg ctgccgatcc cgcaaagacg cgattggcgg cgatcgacag
      841 attgtttacg accgtgctcc cgcgcttcat catgaccagc gccgcctgcg tcaccagaaa
      901 catgccgtcg agattggttt cgaggacatc cctccagacg gggaagggga gcttctctac
      961 cggcagattc gggtgcgcga tgcccgcgtt gttgatgaga atgtcaagcc gctggaattg
     1021 ccgccggatc tggcggaaaa gatcgtcgac cgaatgcgga tcgcggacat cacatccttg
     1081 aacgagcaga cctgccttcg cacgcccaaa ctcccggttg attcgacgca gtcccgcttc
     1141 gctccgtccc gccagaatca gatcgcagcc ttcggccgcc agtgcgtgtg cgattgcgag
     1201 accaatcccg cgcgtcgcgc cggtgactag cgctgctttt ccctgaagcg gcagcaattg
     1261 agcgcggcgg gaaggcatga tagggtcagc caacgatctg atactcggtc ttcaccagcc
     1321 cgctggcgta ggccgtggtt tcaacgtggc gcagttgtac gtcgcgcggc agttcgccaa
     1381 acagcggaat gccgccgcca atcaacaccg gcactcgcgt aatgatcagc cgctgaatca
     1441 gcccttggcg cagaaagcgc tgaatggtca caccaccgtc gacgtagata tgctgccatc
     1501 cgcgagcggc cagtttgccc acgatctcag tgggcgagcc ggacatctgc tccaccacac
     1561 cgtcacgcac cgaagagaaa tccagcggcc ggctgcttaa taccacaacg ggttttttcc
     1621 cgtagggcca cgccgggtag gcgagaacgg tctcaaaagt cccgcgaccg gtaacgatcg
     1681 catccacgct ggcgaaaaac tcgttgtaac cgtggggctc accgccgtcg ggcggaagaa
     1741 agtcgagatc gccgtttggc cgggcgataa atccgtcaac actggtgccg ataaaaactg
     1801 aagcgatcat ggtcggagcc gaacccgtgg ccgcccatga tcgctttttc acctcgtcca
     1861 gtcaaaccct ggtgcgtcag actccattcg cgcgctgccg gatcagcgac aggaactcgg
     1921 agcgcgtctc ctcctcgttg cggaaacatc ccagcatcga cgacgtcacc gcggccgagt
     1981 gctgcttctc gactccgcgc atcatcatgc acaaatggcg cgcttcaatc accacgccca
     2041 ccccttgcgg ctcgatcacc ttctggatgg tttcggcaat ctgcgtggtc agccgctcct
     2101 gaatctgcag gcggcgggaa aagacctcga tcaaccgcgg aatcttgctc agcccgatta
     2161 cgcgcccgtt ggggatatat gccacatgca ctttgccgaa gaagggaagc atgtggtgct
     2221 cgcacaggct gaacatttcg acgtctttca caatgaccat ttcgtcatag gtgactgtga
     2281 acaatgctcc tttaagcagg gcttcgggat cctcgttata accgcgggtg aggtactgca
     2341 tggccttgcg cacccgcttg ggggttgacg tcaggccttc gcgctccgga tcttcgccca
     2401 ggcggatcag catttcgcgc acgagttctt caaaactcgc gctggtcagg gtggctggtt
     2461 caatcgctgg tttcatggtt gggcccatac tcccttgtgg ctgtccgcga gccgcgcccg
     2521 cgggttgccg gtcatcgttt cggttcgtct ccaccggcgt actcgaaaga gttcatcatg
     2581 gtttcgtcga gccgcactct ttccagatgc gccaaatgaa agtctcgctg caatatttcg
     2641 aagatctcga tgcaaagatt ctcagtcgtt ggcacggacc ttgtgaattc ttccagcgta
     2701 ttcagattct ccagatcata gcgggagacg atctcgcgtt ccacacagcc atcgagatcc
     2761 gccaggttgc agaccatgcc ggtgcgatca tcgacgcggc cactgaccgt aacttcgaga
     2821 gcatagttgt ggccgtgccc gtggggattg ttgcattttc cgtaggtggc acggttctca
     2881 tcggcggaca tctcggcact gttcagccga tgtgaggccg aaaaacgata tcgacgtgtg
     2941 agatgagctt tcatattctg aaactcgcaa ccccttgcct caatatcctc agacttgtgc
     3001 tcactcctct ccgtagaagt cgacaaacaa atcgggcgtc tcatacacgc gcacccggtg
     3061 cagccgagcc ttttgtagtt tcggctccaa acgttcccat atcgcgatgg cgaggttttc
     3121 cgtagtcggg atgcgggtgg cgaactccgg aacttccttg tttaggaagc gatgatccat
     3181 cgcgtccagc acttcgccgt gcatggtctc tttcaattgc tttagatcga ccacgaagcc
     3241 cgaccgcgga tcgacttctc ctttcaccgt gacctcgagc gtgtagttgt gtccgtgtcc
     3301 gttcgggttg ttgcacttcc cgaaaacgcg gcgattctct tccgcggtga attcggggtt
     3361 gtggtaatag tgcgacgctg aaaactctgc cttgcgcgtg agataaacca tagggaacca
     3421 gttgccagta atcagtggcc ggtagccagc aagctgcttt tgtccgagta tgtgcggccc
     3481 ttccagccga ccttgccctt ggcatgcgcg cgttgcgact gcagcagcag gtaagcgaac
     3541 atcggcatgc ccacggccac gccaagcaag ttcatgatga aaccgaagtt cgcccgcttg
     3601 atacggataa caagctgaac gaagctgcct gcgaaaatcg cgctccacag ccaggacgga
     3661 cgaatgggat gtctaaggcc aaggaacaga gctgtccacg gcacagccca aagcgctagc
     3721 gtcttcactg ccagccagcc ggggcctggg aatagcagtg ccaaattctt cgtccatccc
     3781 tcgcgcagct gacgatagct gcggtacatt cgcgttcgaa cagcatcgtc cgcatatcgg
     3841 aatcgaatct tccgccccga gctcttgact gctcgcgcca gcgcgacatc ctccaggatc
     3901 tcctcagcaa tcgcgacgtg cccgccgatt gcgtcgtatg cgtctcggcg aatcagaatg
     3961 tactgtccgt tcgccgccgc aatcggtgag gccggatcgc tcactcgtgc cggcggatac
     4021 tgccgcgcca ggtccgcaaa aatcaccggc aacgttgcca tctcccaaaa accaacagcg
     4081 atctgctccg gcgaatatga caacaactcg agcccgttct ctttcgcttc agtcaccgcg
     4141 cgccccagcg accccggcaa gtgcaccgta tcggcgtcgg tgaacagaag ccactcgccg
     4201 cgcgccaggc gcgctccaca ggtgaccgcg ttgttcttac cggtccaccc gtcaggcaga
     4261 ggccccgctt cgatcacacg cacgtcggca aacgacgccg caatttcccg cgtccggtcc
     4321 gccgagtggt cgttgaccac gatgacctca aacggcactt cggtctgcct cagcagcgat
     4381 tccaggcaca tgcccaggca gctttcctca ttccgggccg gcacaatcac cgaaaccacg
     4441 ggctccgccg ccgtctgcaa cgttcctgtg cgggtcgcag ccatacgccg aatccattat
     4501 tatagctggg tgcgacggct cgcctctttc atcagcctct tcgttctgct tgtgtccctg
     4561 tctgcttgtt acagcggcac ccggcctcct cgcattggtt cgacggcgcc cgatttcacc
     4621 gtccaggacg ccgacgccaa ggtcacgctg acccagttcc gcgggcaagt tgtcgtcctg
     4681 aatttctggg cgacctggtg ccctccgtgc attgaagaga tgccatcttt ggttgagatg
     4741 cagcggcgca tgaaagccaa aggcgtgacc gtggttgcaa tcagcattga cgtcgacgac
     4801 aacgcctatc gtcgcttcat caaggatcac gccgccaacc tgctcactgt gcgtgacccg
     4861 gatcaaaaaa ccgcaaatct ctacggcacc cacggctggc ccgaaaccta catcatcgac
     4921 cgcaatggcg tgatgcggcg caagttcatc gggccagtcg attggacgga ccccgagatc
     4981 acgaatttcc tgaacaagct ctagactcgc cactcgcaca ccccgccttt agagagagta
     5041 ctgtctatcc gacccgttac acacgtcgct cacctttcct cttccggcca tgtccaagta
     5101 caatggcgac cgccaatctg gaggaaatct tcaaatgagg aaaactttcg ccgtattcgc
     5161 cgctgctctg cttgccgctg ctttggcgct gcccgcctgc gcgcagga
//
LOCUS       DASPUL010000150         4191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_707229, whole genome shotgun sequence.
ACCESSION   DASPUL010000150 DASPUL010000000
VERSION     DASPUL010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707229"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VEJ00_03675"
     CDS             <1..859
                     /locus_tag="VEJ00_03675"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXX00290.1"
                     /translation="AMGVLYVDSRFASQDLTKGVGQDILHAIATEAASLIENARLVQA
                     EEESRRYQQELSIASGIQQRLMQVRIPDVPFAKMQGRNMQCKEIGGDFYDAVLTNEGL
                     NVILADVSGKGVSAALLATSLQGMIYSHLTSGMSLVDVVTAVNRLFTEKMMGEKYATL
                     LLARLRRDGELEYVNCGHVPPLLVCGGEVIRPPHGNVPVGLLPDATFESASCQMKSGD
                     RFILVTDGVTEAENSMGEFFDDSRLEAAVAKSPTWEGILSAVSEFCAGNPLGDDCTVV
                     ELRYNGAEA"
     gene            complement(976..3525)
                     /locus_tag="VEJ00_03680"
     CDS             complement(976..3525)
                     /locus_tag="VEJ00_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXX00291.1"
                     /translation="MNVSKSWKLLLAGALVGVAVCSPLQARSKSSAGKAAPAPAEASS
                     GYDKPPQYILDVMHAPSPPVPIVSPTYDTILLVSWQDYPPMSRVATPFLRLAGVRVET
                     KNHSKHDTPGGYGITPCARSFDLVRIAEGKQTHVALPAAACPGDPEWAADGKRFAFVN
                     LAADAVELWIGDASTGAAHRVPGARLNPMFDDAMQWMPDQKTLLVKLVPSGMGAPPPA
                     PVVPAGPSIQETEGKSGESSTYENRDTLNSKHDEDLFDYYGASQLALVNATTGAITGI
                     GKVRNYESVDPAPDGKHIFVTTIHKPYSYVTTYDRFPKEVEVWDASDPAHVSSHTIAS
                     LPLADRVPIHGVPTGPREFSWRATDPATLVWAEALDGGDWNVQVPNRDKIMLQKAPFD
                     SPPAEIGRTEQRYVDFSWSEQPSVALRTEYDNNRHWRKSFIVNIDDPQQRPRLLWDLS
                     TDERYKNPGSPVRRQLPNGSRVVRQDGDQIYLSGIGASPDGDRPFLDELNLKTLESKR
                     VFRCDKTSFERFLSFLGADTETFLTWHQSPAEPPNAFKRTLGRSTDAPAGEASFASSS
                     VALTHLPDPTPAVRAIKKRLVKYKRADGLDLSFTLYTPPGYQEGTRVPTILYAYPLDY
                     AEASTAGQVTGSQETFTQLRNYRLLLLAGYAIIDNAAFPIIGDPKKAYDTYLDQLVAD
                     AKAAVDEAVRLGVADPDRIGVTGHSHGALMTVNLVAHSDLFRAGVATSGSYNKTLTPF
                     GFQNERRSVWEATDVYLKVSPFFVADKLKTPLLIMHGAEDANPGTTPIQASKLYEAIR
                     GNGGTARLVLLPHEPHWYTAMESNEQLIYEMLRWFDRYVKNAPPRTQAAAR"
     gene            3761..>4191
                     /locus_tag="VEJ00_03685"
     CDS             3761..>4191
                     /locus_tag="VEJ00_03685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXX00292.1"
                     /translation="MIGQTISHYRIVEKLGGGGMGVVYKAEDTELGRFVALKFLPEDL
                     ARDPQALERFRREARAASALNHPNICTIYEIGKQGEQMFIAMEFLDGVTLKQRILGRP
                     LDLDLLLPAAIEIADALDAAHAAGIIHRDIKPANLFLTTRG"
BASE COUNT          762 a         1190 c         1399 g          840 t
ORIGIN      
        1 ggcgatgggg gttctgtacg tggattcgcg gtttgcctcg caggacctca cgaagggagt
       61 gggtcaggat attttgcacg cgatcgcgac agaagcggcg tcgttgatcg agaacgcccg
      121 gctggtgcag gccgaagaag agtcgcggcg ttatcagcag gaactgagta tcgcatcggg
      181 cattcagcag cggctgatgc aggtgagaat tccggacgtg cccttcgcca aaatgcaggg
      241 caggaacatg cagtgtaagg aaatcggcgg agacttctac gacgcggtgc tcaccaacga
      301 aggcctgaat gtgattctgg ccgatgtgag tggtaagggc gtgtctgccg cgctgctggc
      361 gacctcgctg caaggaatga tctattcgca cctgacttcg ggcatgtcgc tggtggacgt
      421 ggtgacagcg gtgaaccggc tcttcaccga gaaaatgatg ggcgaaaaat atgccacgct
      481 gctgctggcg cgcttgcggc gcgacggcga gctggaatac gtgaactgtg ggcatgtgcc
      541 gccgctgctg gtgtgcggcg gagaggtgat ccgccctccg catggaaatg ttcccgtggg
      601 actgcttccg gacgctactt ttgagagtgc cagttgtcag atgaagtccg gcgacaggtt
      661 cattctggtc accgacggcg tgaccgaggc ggagaattcc atgggcgaat tcttcgatga
      721 ttcgcggctc gaagccgccg tggccaagtc cccgacgtgg gaagggattc tttcggcggt
      781 ctcggaattc tgcgccggca accctctcgg cgacgattgc acggtcgtcg agttgcgtta
      841 caacggcgca gaagcctaga ggccatctca ccaagggctc gtcgcagggg ctaaagcccc
      901 atgtcattct ggggcgctta gcggcgcgct ttaaggcgcg cccttttaga tcagatcatt
      961 ccgtcggcgg cagtgttaac gtgccgcggc ctgtgtgcgc ggcggggcgt tcttcacgta
     1021 cctgtcgaac cagcgcagca tctcgtaaat cagctgttcg ttcgactcca tcgccgtata
     1081 ccagtgcggt tcgtgcggca gcagcacgag ccgagccgtc ccgccgttgc cgcgaatcgc
     1141 ctcgtagagc ttgctcgcct ggatgggcgt cgtaccggga ttcgcgtcct ctgcgccgtg
     1201 catgatgagc agcggcgtct tcaatttgtc ggcgacgaag aacggggaaa ctttcagata
     1261 gacatccgtc gcctcccaca ccgagcggcg ttcattctga aaaccgaagg gggtgagggt
     1321 tttgttgtaa gagccgctcg tggccactcc agcgcggaac aggtctgagt gcgcgaccag
     1381 attcacggtc atgagtgcgc cgtgactgtg accggtgact ccgatgcggt cggggtcggc
     1441 gacgcccagg cgcaccgctt catcaaccgc ggccttggcg tcggccacca gctggtccag
     1501 ataagtgtcg taggccttct tgggatctcc gatgatcgga aatgcggcgt tgtcgatgat
     1561 ggcgtagccg gcgagcagca ggaggcggta attccgcagc tgtgtgaagg tctcctgcga
     1621 gcccgtaacc tggcccgcgg ttgacgcctc ggcatagtcg agaggataag cgtagagaat
     1681 cgtgggaacg cgcgtgccct cctgatagcc gggcggagta tagagcgtga acgacaaatc
     1741 gagcccgtcg gcacgtttgt acttgaccag gcgctttttt atggcgcgca ctgccggcgt
     1801 gggatcgggg agatgggtaa gcgcgacgct gcttgaggcg aaactcgcct ctccggctgg
     1861 ggcatcggtg gatcgtccca gcgttcgctt aaacgcattc ggcggctcgg ccggcgattg
     1921 atgccaggtg agaaacgtct ccgtgtccgc gccgagaaaa gaaagaaagc gctcgaacga
     1981 agtcttgtcg cagcggaata cgcgtttgga ctcgagcgtt ttgagattca gctcatcgag
     2041 gaatggccgg tcgccgtccg gcgaagcgcc tatccctgaa aggtagatct ggtcgccgtc
     2101 ctggcgaacc acccggctgc cgttgggcaa ctggcgtctc acgggagagc cggggttctt
     2161 gtaccgctcg tcagtcgaga gatcccaaag caggcggggc ctttgctgcg gatcgtcgat
     2221 gttgacgatg aagctctttc tccaatgccg gttgttgtcg tattcggtcc ggagggcgac
     2281 gctcggttgc tcgctccacg agaagtcgac ataacgctgc tcggtgcgac cgatttcggc
     2341 gggcggcgag tcgaaaggcg ccttctgcag catgatcttg tcgcggttcg gaacctgcac
     2401 attccagtcg ccgccatcga gcgcttccgc ccaaactaac gttgcgggat cggtggcacg
     2461 ccaggagaac tcgcgcggac ctgtgggaac gccgtgaatg ggaacccgat ctgccagggg
     2521 aagcgaggcg atggtgtggc tggaaacgtg ggcgggatcg gacgcgtccc agacttctac
     2581 ttcttttggg aagcggtcgt aggtcgtgac gtaggaatag ggcttgtgga tcgtggtgac
     2641 gaagatgtgc ttgccgtccg gcgcgggatc gacagattca tagttcctta ccttcccgat
     2701 gcccgtgatt gctccagtcg tggcgttgac cagggcgagc tgggaggcgc cgtagtaatc
     2761 gaagagatct tcgtcatgct tgctgttgag cgtgtcgcgg ttctcgtagg tgctgctctc
     2821 gccggatttg ccttcggttt cctggatgct tggaccggcg ggcacgacag gagcaggcgg
     2881 cggtgcgccc atgccactgg gcaccagctt gaccagtagt gttttctggt cgggcatcca
     2941 ttgcatggca tcgtcaaaca taggattgag gcgtgcgccg ggcacgcggt gtgccgcgcc
     3001 ggtgctcgcg tcgccgatcc agagttcgac tgcgtcggcg gcaagattga cgaaagcaaa
     3061 tcgtttgcca tcggctgccc actccggatc tccagggcac gccgccgcag ggagcgcgac
     3121 gtgagtctgt ttgccctcgg cgatgcgcac caggtcgaag ctgcgggcac agggcgtgat
     3181 gccgtagccg cccggggtgt cgtgcttgct gtgattcttc gtctcgacgc gcacgccggc
     3241 caggcgcaag aagggagtgg ccacgcgcga catgggcgga tagtcctgcc atgacacgag
     3301 cagaattgtg tcgtacgtcg ggctcacgat gggaactgga ggcgacggcg cgtgcatcac
     3361 atccagaatg tactgcgggg gcttgtcata gccggacgag gcctcggccg gggctggagc
     3421 cgcctttccg gcggatgatt tcgaccgcgc ttgaagcggt gagcacaccg ccacgccaac
     3481 cagcgctcct gccaatagca gcttccagga tttgctcacg ttcataggct cttctgcccc
     3541 cctcacaacc gaaagtgcga catagagcat actccttgac cgtgcgggct gtctgaatgt
     3601 gcaggcgtcg cccagagagg aaggctgcgc aatgcgccgt cgggcgaatg atcaggacgc
     3661 acgacaggcg gagaatttga cttcgcccgg ttccgtcgcg gccgcggatc gtcgcgtgtt
     3721 tcattgactc gccgtttggg tgatcgtaag attcccgcca atgatcggcc agaccatctc
     3781 gcactaccgc atcgtggaga aactcggcgg cggtggcatg ggagtcgtct acaaggccga
     3841 ggacaccgaa ctggggcgct tcgtcgccct gaagttcctg ccggaggacc tcgcccgcga
     3901 cccgcaggcg ctggagcggt ttcgccgcga agcccgggca gcatcggcgc tgaaccatcc
     3961 caacatctgc accatctacg aaattggcaa gcaaggcgag cagatgttca tcgcgatgga
     4021 gtttcttgat ggcgttacgc tgaagcagcg catcttgggg cgtccgctgg atctggacct
     4081 gttgttgccg gcggccatcg aaatcgccga tgcgctcgac gcggcccacg ccgccggcat
     4141 cattcaccgc gacatcaagc ccgcaaatct gtttctcacc acacggggtc a
//
LOCUS       DASPUL010000151         6954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_844511, whole genome shotgun sequence.
ACCESSION   DASPUL010000151 DASPUL010000000
VERSION     DASPUL010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844511"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1463)
                     /locus_tag="VEJ00_03690"
     CDS             complement(117..1463)
                     /locus_tag="VEJ00_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXX00293.1"
                     /translation="MALKTYGTMAVDWEKRIDFDRLRRERLQKAKDLLAKSSMGALLC
                     FDMNNVRYLTATHIGTWAQDKANRFTLLPQNDEPILWDFGSAAKHHQLNCPWLDGRSR
                     PGISMLRGAMTPQMGRAEDVARKIRIELEMRGLHKEPVGIDIIELPVLFALQREGIKV
                     VDGQELMSEARVIKTQDEIALLNHSAMMVDAAYDELYRAMKPGLRENEAVGLVSKVLY
                     DLGSEYVEAVNAISGERCNPHPHVFSDRVLRPGDPVYYDILHSYMGYRTCYYRCFTVG
                     YASHAMVDAYKRCREYLDAAIELVRPGRTTAEIAAVWPKAEEFGFPNEEACFALQYGH
                     GVGLAIWEKPVISRLVSFEHPVEIKPGMVFALETFWPSTDGWSAARIEEEIVVTPTGH
                     EVITRFPAEQLLVAGAHYFTVNGPLPTTRESEAPPSDRVIEMIEASAKSERVGVSS"
     gene            complement(1536..2519)
                     /locus_tag="VEJ00_03695"
     CDS             complement(1536..2519)
                     /locus_tag="VEJ00_03695"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HXX00294.1"
                     /translation="MSERELTFAQAVREALAEEMRRDTRVCIFGEDVAEAGTPFKVLS
                     GLVEEFGAERVIDTPISEAGFTGIGVGAAMTGLRPVVDIMFGDFLTLTMDQMVNQAAK
                     VHYMSGGHWRVPMVLRTTLGATRRSAAQHSQSLQAWFSHVPGLKVVLPSTPYDAKGLL
                     KTAIRDENPVVFFEDKMMYKLKGPVPEDDYTIPLGVADVKRAGDDITLVATSSMVQVA
                     LGAANLLEKEGISAEVVDPRTTWPLDERTIIESVKKTSRAIVLDEGYGRYGVTSEIAA
                     VIAEGAFYDLDAPVKRMGAMHVPIPFSPPLEDVTVPTEESVREAARQMCRK"
     gene            complement(2528..3532)
                     /locus_tag="VEJ00_03700"
     CDS             complement(2528..3532)
                     /locus_tag="VEJ00_03700"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HXX00295.1"
                     /translation="MTTATSKAEADRQAATEKDKWLRAYRQMVRIRLFEEQVNELYTR
                     ALMPGLAHLYSGEEAVAVGICEALRIDDYITSTHRGHGHCLAKGASPDRMFAELLGKE
                     AGYCRGKGGSMHIADPATGNLGANAIVGGSVGIATGAALAAKRLGNGRVAVCFFGEGA
                     LGQGSLYEVMNLAQLWKLPVIYVCENNLYNEYTHYSETTAGTILGRAKAFDLEATIVD
                     GQDVRAVHEVATRLVQRARVGGGPAFLQADTYRYSGHHVGDINREYYRSKQEEQFWKT
                     ERDPIRLHAGWLVARGYADSPSLKTIDAEARGEMEAAVKFAIEAPYPSVDQVNQDVYA
                     "
     gene            complement(3529..4437)
                     /locus_tag="VEJ00_03705"
     CDS             complement(3529..4437)
                     /locus_tag="VEJ00_03705"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXX00296.1"
                     /translation="MAKIGFIGLGVMGSQMVNRLLGKGHSVTGYNRTRTKAQWLVEKG
                     MQWADSPRAVASASDFIFAMVTNSAAIHAVTEGPDGLLAGLSAGKVFIDMSTISPEVS
                     RALAAKVRSLGADMVDSPVSGSVITLQEGKLSVMVGGRKETFEKIKPLLLDIGPKVTH
                     VGDNGLALCMKIATNLNLAVQMLAFSEGVLLAEKSGIAREVAVDVLTHSAIASPMIQY
                     RGPFVLKQPEEAWFDVNMMQKDMVLAMDLGRQLDVPLPTTAASNEFLTAARGMGWTKY
                     DFACMFDVLATMAGVTTPVTAGGKKK"
     gene            complement(4483..5208)
                     /locus_tag="VEJ00_03710"
     CDS             complement(4483..5208)
                     /locus_tag="VEJ00_03710"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HXX00297.1"
                     /translation="MYKLVRTSRLYEQIVQQIEESIVKGDLKPGDQLPAERDLAQRFG
                     VSRTAVREAVKALREKGLVEAYSGRGTFVTDGTRNAVRQSLDLMSRMGQQEGSTHLAE
                     VRAILEPEIAALAAQRIQESELTTMREAVAVMDKAGQDPDAYIEADLDFHLALAEAAA
                     NPLILSLIDSIVGLLREQRLRIFKVSGGPERGQVHHKQILEAIEARDADKARDAMRAH
                     LEQVREDSQASAGKRGSGRQSAH"
     gene            5556..>6954
                     /locus_tag="VEJ00_03715"
     CDS             5556..>6954
                     /locus_tag="VEJ00_03715"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXX00298.1"
                     /translation="MLLRTVGATMSRCEKMLITRWLIALSTLVLLAVPSLGQSTAGRV
                     LGTITDQSGAAVAGATVVLTDVERGTVRSLSTDQSGTYVAADLQPGTYKIHVEAKGFK
                     TVERPSVPVEVATDVRADFTLTPGQVNETITISEDIPLVNTTSATLGGTLSNQEINDL
                     PLNGRNYQNLLQLRPGVMRYPGGGFSTTSANGLRAEDNAYLIDGLYNTEPFSGQGVIN
                     GSGVVGDSATILPIDAIQEFNLLQNPPAEYGWKPGAIVNVGLKSGTDSLHGTAFAFGR
                     DGAMDARNFFNTPPASKVDRSLEQFGGSLGGAIIKDKAFFFASYEGQRYNIGNTYFVT
                     TPSMVSFTSAGGAGCTSLTSGDCGGSIPDAVHDLLLNGFTIQATGPCTGPCISPESLK
                     LSGCAVSGSGATATVTCNGSGFPVNNNPTINFVQGFPNVANTDDVVAKVDFNVTQRHT
                     ISGTYFFGNNSGTAED"
BASE COUNT         1291 a         2277 c         1929 g         1457 t
ORIGIN      
        1 gccttctttc tttcgccagg cgatgagctt gccggtctcc tgcgccatct cgagggcggg
       61 cattactacg ctgaaggcca tagtcgtttg tgtcggggga aggcggacta cggccttcag
      121 ctcgaaactc ctacgcgctc gctcttggcg ctagcctcaa tcatctcgat tacgcgatcg
      181 ctgggcggag cttcgctttc tcgtgtggtg ggcagcggcc cgttcacggt gaaatagtgc
      241 gctccggcaa ctaagagctg ttctgccggg aagcgcgtga tgacttcgtg tcctgtcgga
      301 gtgaccacaa tttcttcctc gatgcgcgcc gcgctccacc cgtcggtcga gggccagaac
      361 gtttcaagcg caaacaccat gcccggcttg atctccaccg gatgctcgaa cgacacgagg
      421 cggctgatga ccggcttttc ccagatcgcc agccccacgc catgtccgta ttgcagcgcg
      481 aaacaggcct cttcattggg gaaaccgaac tcttcggctt tcggccacac tgccgcaatt
      541 tccgcggtgg tgcggccggg acgcaccagt tcgattgccg catcgagata ttcgcggcag
      601 cgcttgtagg cgtcgaccat ggcatgcgac gcgtagccaa ccgtgaagca gcggtagtag
      661 cacgtgcggt atcccatgta ggaatgcaga atgtcgtagt acacgggatc gccggggcgc
      721 aacactcggt cggagaacac gtgtggatgc ggattgcagc gctcgcccga aatcgcgttc
      781 acggcctcga catactctga gccgaggtcg tagagaacct tgctgaccag gccgactgct
      841 tcgttctcgc gcagcccggg tttcatcgcg cggtagagtt catcgtaagc cgcgtccacc
      901 atcatcgccg agtggtttag cagcgcgatt tcatcctgcg tcttgatcac tcgcgcctcg
      961 gacatcaatt cctgcccgtc gacgactttg attccttcgc gctgcaatgc aaaaagcacc
     1021 ggcaactcga tgatgtcgat gcccaccggc tctttgtgca gaccccgcat ttcaagctcg
     1081 atgcgaatct tcctggcgac gtcttcggcg cgacccatct gcggcgtcat cgccccacgc
     1141 agcatcgaaa tcccggggcg cgagcggcca tcgagccacg ggcaattcag ctgatgatgc
     1201 ttggcggccg agccaaaatc ccacaggatc ggctcatcgt tttgcggcag cagcgtgaag
     1261 cggttggcct tgtcctgcgc ccacgtgcca atgtgcgtcg ccgtcagata gcgcacattg
     1321 ttcatgtcga agcacagcag cgcgcccatc gacgacttgg ccagcaggtc tttcgctttt
     1381 tgtaggcgct cgcggcgcag ccggtcgaag tcgatccgct tttcccaatc caccgccata
     1441 gttccgtaag ttttgagggc catgacctac tccttagcta tgagccatta gcggcgagca
     1501 gccggttgcc tcgctcatag ctcacggctc atcgctcatt ttctgcacat ctggcgggca
     1561 gcttcgcgta cgctttcttc cgtcggtacc gtcacatcct ccagcggagg ggaaaaagga
     1621 atcggcacat gcattgcgcc catccgcttt accggcgcgt cgaggtcgta gaaggcacct
     1681 tcggcgatca ccgctgcaat ctccgacgtg actccgtagc gcccgtaacc ttcatcgagc
     1741 acaatggcgc gcgacgtctt cttcaccgat tcaatgatag tcctttcatc gagcggccaa
     1801 gtggtgcggg ggtctacgac ctccgcgctg atgccttcct tttccagcag atttgccgca
     1861 cccagcgcga cctgcaccat gctgctggtg gccacgagcg tgatgtcatc gccggcacgc
     1921 ttcacgtcag ccacgcccag ggggatcgtg taatcatcct ccggcacagg gcctttcagc
     1981 ttgtacatca ttttgtcttc gaagaagacg acggggttct catcgcggat cgcagtcttc
     2041 agcaggcctt tcgcgtcgta cggagtcgaa ggcaacacga ccttcagccc tggcacgtgg
     2101 ctgaaccatg cctgcagcga ctgcgaatgc tgcgctgcgg agcgccgggt cgcgccgagc
     2161 gtcgtacgca gtaccatcgg cacgcgccaa tgcccgcccg acatgtagtg gactttcgcg
     2221 gcttggttga ccatctggtc catagtcaac gtcaggaaat cgccgaacat gatgtcgacg
     2281 acgggccgca gaccggtcat ggcagcgccc accccgatcc ccgtgaagcc ggcttcggaa
     2341 atcggcgtgt cgattacgcg ctcggcgccg aattcttcta cgaggccgga aagcaccttg
     2401 aatggtgttc ccgcttcggc gacatcttcg ccaaaaatgc aaacgcgtgt atcacggcgc
     2461 atttcctcgg ccagcgcctc gcgcacggct tgcgcaaatg tgagttcacg ctccgacata
     2521 gaaactttca ggcatagacg tcctggttga cttgatcaac gctggggtac ggtgcctcaa
     2581 ttgcaaactt caccgcagct tccatctcgc cgcgtgcttc cgcgtcaatc gtcttcagtg
     2641 agggcgagtc cgcatagccc cgcgccacca gccatcccgc gtgcagcctg atgggatcgc
     2701 gctccgtctt ccagaactgc tcttcctgct tcgagcggta atattcgcga ttgatgtccc
     2761 cgacgtgatg accgctgtag cggtacgtgt cagcctgcag gaacgccggc cctccgccca
     2821 cccgcgcgcg ctgcaccagc cgcgtcgcga cttcatgcac tgcgcgcaca tcctgtccgt
     2881 cgacgatcgt tgcttcaagg tcgaaggcct tggcccgccc cagaatcgtc ccggcagtgg
     2941 tctcgctgta gtgcgtgtac tcgttgtaga gattgttctc gcagacgtag atgacgggaa
     3001 gcttccagag ttgggccagg ttcatcactt catacagcga tccttgaccg agcgcccctt
     3061 cgccaaaaaa gcaaaccgcc acgcgtccgt tccccagccg cttcgccgcg agagccgcgc
     3121 cggtcgcaat gcccacgctt cccccgacga tcgcattcgc accgagattg ccggttgcgg
     3181 gatcggcgat atgcatcgat ccgcccttac cacggcagta ccccgcttct ttgcctaaca
     3241 actcggcaaa catgcgatcg ggcgatgcgc ctttggccag gcagtgtccg tggccgcgat
     3301 gcgtgctggt gatgtagtcg tcgatgcgca gcgcctcgca gatcccaacc gcaaccgcct
     3361 cttcaccgct gtagaggtgg gcgagcccag gcatcagggc gcgagtatag agttcgttta
     3421 cctgctcttc gaacaatcga atacgcacca tttggcgata ggcgcggagc cacttgtctt
     3481 tttccgtggc tgcctgccga tcggcttccg ccttggacgt agccgttgtc acttcttctt
     3541 ccctccggcg gtcacaggcg tcgtcacgcc cgccatggtg gcgagcacgt cgaacatgca
     3601 ggcgaaatcg tatttcgtcc agcccatgcc gcgggctgcc gtcaagaact cgttgctcgc
     3661 ggcggtggtt ggtagcggca catcgagttg gcggcctaaa tccatggcca gcaccatgtc
     3721 tttctgcatc atgttgacgt cgaaccaggc ctcttccggc tgcttcagca cgaatggacc
     3781 acggtactga atcatcggcg aagcgatggc actgtgtgtg agcacatcga cggcgacctc
     3841 gcgcgcgatg ccgctttttt ccgccaggag cacgccttca gaaaacgcaa gcatctgcac
     3901 ggccaggttc aggttggtgg cgattttcat gcacagcgcc aggccgttgt cgccaacgtg
     3961 agtgactttc gggccgatgt caagcagcag cggcttgatc ttttcgaacg tttctttcct
     4021 tccaccgacc atcaccgaca gcttgccttc ctgcagcgtg atgacgctcc cggaaaccgg
     4081 agaatcaacc atgtcggccc cgagagaccg cactttcgcc gccagcgcac ggctcacctc
     4141 ggggctgatc gtgctcatgt cgatgaagac cttgccagca ctcagacctg ctagcagtcc
     4201 gtccggccct tcagtgaccg cgtggatcgc cgccgagttc gtgaccatgg cgaagatgaa
     4261 atctgacgcg ctggccactg cccgcggcga gtcggcccac tgcatgccct tctccaccag
     4321 ccactgcgcc ttggtccggg tgcggttgta gccggtcacg ctatggccct tgccgagcaa
     4381 ccggttcacc atctggctgc ccatcacccc caggccaatg aatccaattt ttgccatccg
     4441 ctaccgcctc cccatctccc gttcgctgcc gcagcactct ttctaatgcg cgctctgcct
     4501 gcccgagccg cgcttgcccg ccgaagcctg actgtcctca cgtacctgct ccaggtgcgc
     4561 gcgcatagcg tcgcgggctt tgtctgcgtc gcgggcttcg atcgcttcga gaatttgctt
     4621 gtgatgcact tggccgcgct cgggcccgcc ggacactttg aagatccgca gccgttgctc
     4681 ccgcaacagc ccgacgatgg aatcaatcag cgaaagaatc agcggatttg cagcagcttc
     4741 ggccagcgcc aggtgaaaat ccagatcggc ttcaatgtag gcatcgggat cctgcccggc
     4801 cttgtccatg accgccaccg cttcgcgcat cgtggtcaat tccgactcct gaatgcgctg
     4861 agcagccaat gcggcaatct caggttccag aatcgctcgc acttctgcca ggtgcgtcga
     4921 cccttcctgc tggcccatgc ggctcatcag gtcaagcgac tgtcggaccg cgttccgtgt
     4981 tccgtcggtg acgaatgtgc cgcgcccgga atacgcctcc accaggcctt tctcccgcag
     5041 cgctttcacg gcttctcgta ctgcggtgcg gctgacccca aagcgctgtg ccaggtcgcg
     5101 ttccgccggc agttggtcgc ctggtttcag atcgcctttg acgatcgatt cctcgatttg
     5161 ttgcacgatt tgttcgtaga gtcgcgacgt gcgaactagc ttgtacactg cccggccccc
     5221 tgcgcggtgc ggaaccacag caccgctggt gatggtccgg ctactatacc gcagatccac
     5281 cgggatcagg cgcgtttgtc cgaagctgac caaggcttgg tatatgggta tggtggtatg
     5341 accgcaaaca tcctactacg ccaccacgcc gccctgcaac tctgaccaca agactaaaaa
     5401 ctccagccac ttagacgggt tgccgcatta tggactcccc tttccttcgc cttgggagta
     5461 cgctcggctg gccgatccac gaattgaatt tgcttgacag cgtttctggg atagtggtat
     5521 ggctgtcgga ccagaaacgc cagagagggg ttcccatgct gcttcgcacc gtcggcgcaa
     5581 ctatgtcccg ctgcgagaag atgctcatca cccgctggtt gattgctctt tccacgcttg
     5641 tcctgctcgc cgttccgtct ttaggccagt ccacggccgg ccgcgttctc ggcacaataa
     5701 ccgaccagag cggcgccgcg gtcgcaggcg cgactgtcgt ccttaccgac gtcgagcgcg
     5761 gcacggtgcg cagtctgtca accgaccagt ctggaaccta cgtcgcagcc gacctccagc
     5821 ccgggaccta caagatccac gtagaagcga agggattcaa aaccgtggag cgccccagcg
     5881 ttcccgttga ggtcgctacc gacgtgcgcg ccgactttac cctgacgccc ggccaagtca
     5941 acgagaccat caccatctcc gaggacattc ctctggtaaa caccacttcg gcgacgctgg
     6001 gcggaactct gagtaatcag gagatcaacg atctcccgct caacggccgc aactaccaga
     6061 acctgctgca actgcggccg ggcgtcatgc gctaccccgg cggcggcttc tccaccacta
     6121 gcgccaacgg actgcgcgcc gaagacaatg cttacctgat cgacggcctc tacaacactg
     6181 aacccttctc cggccagggc gtgatcaacg gctctggagt ggtaggcgac tcggccacca
     6241 ttcttccgat tgacgccatt caggaattca acctgctgca gaaccctccc gcagagtacg
     6301 gctggaaacc cggtgcgatc gtcaacgtgg gattgaagtc cggcactgac tcgctgcacg
     6361 gaacggcatt tgcttttggc cgcgacggag ccatggatgc cagaaacttc ttcaatactc
     6421 ctccggcttc aaaggtcgac agaagcctgg aacaattcgg cggaagtttg ggcggagcca
     6481 tcatcaaaga caaggcattc ttcttcgcat cctacgaagg ccagcgctac aacatcggga
     6541 acacctattt cgtgaccact ccgtcgatgg tgtcgttcac atcggctggg ggagcaggct
     6601 gcactagcct aacaagcggt gattgcggcg gcagcatccc cgatgcggtt cacgacctct
     6661 tgctaaatgg cttcacgata caggcgaccg ggccttgcac tggaccgtgc atcagtccag
     6721 agagcctcaa gctttccggt tgcgccgtct ctggctctgg cgctacggcc accgtcacct
     6781 gcaatggatc cggcttcccg gtcaacaaca atcccacgat caactttgtt cagggatttc
     6841 ccaacgtagc caacaccgac gacgtggtcg cgaaagtaga tttcaacgtt actcagcgcc
     6901 ataccatcag tggcacatat ttcttcggaa acaactcggg cacggccgag gact
//
LOCUS       DASPUL010000152         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1393292, whole genome shotgun sequence.
ACCESSION   DASPUL010000152 DASPUL010000000
VERSION     DASPUL010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1393292"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VEJ00_03720"
     CDS             <1..274
                     /locus_tag="VEJ00_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00299.1"
                     /translation="KFAWIGAFSSGGLPGDFQTDFPALDAKANQQIRLLWIACGTEDH
                     LITANRNFREWLKSKGMEHKGIETPGEHTWMVWRRNLAEFAALLFR"
     gene            287..739
                     /locus_tag="VEJ00_03725"
     CDS             287..739
                     /locus_tag="VEJ00_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00300.1"
                     /translation="MTKRICAIGLLVSLCAAATSAALAQTHGCTVEKMKQAEKETENL
                     KSWELVYEFYQTYSRCDDGGVAEGVSDKIAKLLANHWSSVAEFVSIANNDKKFEKFVL
                     RHVDATIDWEHDGPKIHENAKMHCPVNLKQMCRELMARSTPPRDMGPK"
     gene            complement(749..>2365)
                     /locus_tag="VEJ00_03730"
     CDS             complement(749..>2365)
                     /locus_tag="VEJ00_03730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXX00301.1"
                     /translation="DWSSNPRRSAEQITSYVLNHLPPCRTDDLRCGNIVLLHDGGGDR
                     SETVRALPMIIDGIRARGYEIAPVYDLIGKTRADVMAPLPTGELWAARLDSLGFWLFD
                     AGVVGITWIFLLGDLLMTGRLIFIGAAAVYDRLHEKIFGRPAELASYKPKVAVLIPAY
                     NEEKVIERTVRAALNSNYPNLRVIVIDDGSRDRTLEVAREAFAREAASGRVLILTKLN
                     SGKAEALNYGIEHIGDAELFVGIDADTVIAPDAISRLVPHFINPRVGAIAGNAKVGNR
                     VNLWTRWQALEYITSQNFERRALDVLGAVSVVPGAIGAWRVSAVREAGGYHIDTVAED
                     ADLTMALLRLGYRVEYEDMALAYTEAPTNANGLMRQRFRWSFGILQAVYKHRRVVARK
                     GALGWVALPNIVIFQILLPLVSPLIDIMFAVGTIWYLIQKHFHPDSTDPASFHKLVAF
                     FFAFLVIDFLASVIAFALERRRPDGKEDVWLLSQVWLQRFAYRQLFSIVLFKTLKRAL
                     EGRKFAWDKLERTAAVKYVPAESPDHISVP"
BASE COUNT          499 a          638 c          802 g          426 t
ORIGIN      
        1 caagttcgcc tggatcgggg ccttcagttc gggcggtctg cccggagatt tccagaccga
       61 cttccccgcg ctggacgcga aggcgaacca gcagattcgg ctgctgtgga tcgcctgcgg
      121 gactgaagat catctgatca cggcgaatcg caacttccgc gagtggttga agtcgaaggg
      181 catggaacac aaaggcatcg aaacgccggg agagcacacg tggatggtgt ggcggcggaa
      241 tctggcggag tttgccgcgc ttctgttccg gtgaagggag gtcgcgatga caaaacgaat
      301 ttgcgccatt ggcctgctgg tgtccctttg cgccgccgcg accagcgcgg ccctcgcgca
      361 aacgcacggc tgcaccgtgg agaaaatgaa gcaggccgag aaggagacgg aaaatctcaa
      421 atcatgggag ttggtgtacg agttttacca gacctattcg cggtgtgacg acgggggcgt
      481 ggcggaggga gtgagcgaca agatcgccaa gctgctggcg aatcactgga gctcggttgc
      541 cgagtttgtg tcgatcgcga acaacgacaa gaagttcgag aagtttgtcc tgcggcatgt
      601 ggatgcgacg atcgactggg agcatgacgg ccccaagatc catgaaaatg ccaaaatgca
      661 ttgccccgtg aacctgaagc agatgtgcag ggaattgatg gcgagatcga cgccgccgcg
      721 cgacatgggg ccgaagtagc ttcgtaggtc agggcacgct gatgtggtcg gggctttccg
      781 ccggcacata ctttacggcg gcggtgcgct ccagcttgtc ccaggcgaac ttccttcctt
      841 cgagcgcgcg cttcagcgtc ttgaacagca caatcgagaa caactggcga taggcgaagc
      901 gctgcagcca gacctggctg agcagccaga cgtcttcttt gccgtcggga cgccggcgct
      961 cgagagcaaa cgcaatcacc gaggcgagga aatcaatcac cagaaacgcg aagaagaatg
     1021 cgaccagctt gtggaagctg gcgggatcag tggagtcggg gtggaaatgc ttctggatga
     1081 gataccagat cgtgcccacg gcgaacatga tgtcgatcag cggtgagacc agaggcagga
     1141 gaatctgaaa gatgacgatg ttgggcaaag cgacccatcc caaggcgcct ttgcgggcca
     1201 caacccggcg atgcttgtaa accgcctgca ggatgccgaa ggaccaccgg aaacgctggc
     1261 gcatcaggcc atttgcgttg gtgggggctt cggtgtaggc gagggccata tcttcgtact
     1321 cgacgcggta gccgaggcgc agcaatgcca tggtaaggtc ggcgtcctcg gcgacggtgt
     1381 cgatgtgata gccgccggct tcgcgaacgg cggaaacgcg ccacgcgccg atggctcccg
     1441 ggacaacgct gacggcgccg agaacatcga gagcgcggcg ctcgaagttc tggctggtga
     1501 tgtattcgag ggcctgccag cgcgtccaca ggttgacgcg gttgccgacc ttggcattgc
     1561 ccgcgatggc gccgactctt gggttgatga agtgcggcac caggcgcgag attgcgtcag
     1621 gggcgatgac cgtgtcggcg tcgatgccga cgaaaagctc cgcgtcaccg atgtgctcga
     1681 tgccgtagtt gagggcctcg gccttgcccg agttcagctt ggtgaggatg agaacccgtc
     1741 ctgaggcggc ctcgcgggca aaggcttcgc gcgcgacttc aagcgtgcgg tcgcgtgagc
     1801 cgtcatcgat gacgatgacg cgcagattgg gatagttgga attcagcgcg gcgcggacgg
     1861 ttcgctcgat gactttctct tcgttgtagg cggggatcag cactgcgact ttaggcttgt
     1921 aggaagctag ttccgcgggg cgtccgaaga ttttttcgtg caaacggtca tagacggcgg
     1981 cggctccgat gaaaatcagg cggccggtca tgagcaggtc gccgagcaaa aagatccagg
     2041 tgatgcccac tacaccggcg tcaaaaagcc agaaaccgag gctgtcgagc cgggctgccc
     2101 agagttcgcc cgttggcaaa ggcgccatga catcggctcg cgtcttgccg atcaaatcgt
     2161 agacgggagc gatctcatag ccgcgagcgc ggatgccgtc gatgatcatg ggcaaggcgc
     2221 gcacggtttc agagcgatcg ccgccgccat catgcagcag cacgatgttg ccgcagcgca
     2281 gatcatccgt tcggcaaggc ggcagatggt tgagtacgta agaagtgatc tgttcagcgg
     2341 aacgccgggg attgctgctc cagtc
//
LOCUS       DASPUL010000153         4820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_575930, whole genome shotgun sequence.
ACCESSION   DASPUL010000153 DASPUL010000000
VERSION     DASPUL010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575930"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..740)
                     /locus_tag="VEJ00_03735"
     CDS             complement(144..740)
                     /locus_tag="VEJ00_03735"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HXX00302.1"
                     /translation="MDGMAPDVTVFILAGGKSTRMGADKAFVALDGRTLLARALELAR
                     SVTGDVRIVGDAAKFGAFAPVVEDVFRDCGPLGGIHAALRASQEELNLVLAVDVPFVS
                     LALLQYLITRARGSLATVTVARAGGGWQPLCAVYRREFGDVAESALRAGRYKIDALFD
                     AQRTQVIGEEELEVAGFAPRIFRNLNTPEELAEASGGC"
     gene            1129..1653
                     /locus_tag="VEJ00_03740"
     CDS             1129..1653
                     /locus_tag="VEJ00_03740"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXX00303.1"
                     /translation="MPQKIMDVLMLGRCSHEFSWPRRGADGDYYQVCLLCAAEYKYDW
                     TTMRRTERVEHKPESGSRRGHTREKRPSWVPRARRLKLDTPLRYRVNRTSAWHEGIIE
                     NLSQSGVLFHGPQQLPVNALIEMVFEMPEEISGQKNSNVVCQGRVMRAKDQGETEEGH
                     VSLAASIVDYKFIH"
     gene            1797..2900
                     /locus_tag="VEJ00_03745"
     CDS             1797..2900
                     /locus_tag="VEJ00_03745"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXX00304.1"
                     /translation="MKRAPLIISSCVLAIILAITVGCAKKPDDAKISSDVQGRFSQDS
                     GLSTKQLTVQSANGVVTISGTVDNDAQRDAAARQAAAVAGVKEVVNNLQVAGAAPAPP
                     AAAPAPEAAPAQTATAPPAEKPKPAPGKKSHRSHASQNADNGDNSAASQMAGNQAPPP
                     TAQAAAPQDTAMAPPPPPPPPPKKFIIDQGTQITVRLIDPIDSEKNQTGDTFHATLNA
                     PLTSDGEEAVPAGVEVTGHLVEVKSAGKFAGQSTVAMQLDSIASGGQTYNVQSDIYRK
                     SGSSRGKNTAEKVGGGALVGTLIGALAGGGKGAAIGAAAGAGVGGGVQAATKAQQIKF
                     PSETVLTFTLQAPVTVIQAPKPDSNRQKLSTSQ"
     gene            3053..3532
                     /locus_tag="VEJ00_03750"
     CDS             3053..3532
                     /locus_tag="VEJ00_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00305.1"
                     /translation="MPSVTALLHTQNDALRLGRCLETLYPCDEIVIIDHASRDLTVRI
                     AREYGAQVVRPVPGASPNFYVPPSDSTWLLCLAPCESLSEGLAASLFEWKELPSLPSA
                     AAFSVLVRDEAVSGWADIAALQTRLVSSRWKRWEGTLPTNDPSAAVLEGELLHFVLP"
     gene            3669..4106
                     /locus_tag="VEJ00_03755"
     CDS             3669..4106
                     /locus_tag="VEJ00_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXX00306.1"
                     /translation="MKFISLVLVVMTISALAVAQQAPAQTPPEVKHVPITSTPSNSGK
                     EMFESYCAVCHGKDGKGNGPAASAMKTPPSDLTGLAKKEGGKYPSAHVAAVIRGQATT
                     PSHGSQEMPVWGPLFSSISQGHEAQVQQRVTNLVKYIETLQAK"
     gene            4534..>4820
                     /locus_tag="VEJ00_03760"
     CDS             4534..>4820
                     /locus_tag="VEJ00_03760"
                     /inference="COORDINATES: protein motif:HMM:NF018460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HXX00307.1"
                     /translation="MDPVFTAAIFVTGLAFGSFLNVCIYRLPLGISVVSPRSACPRCK
                     HGIALYDNIPVLSWLVLRGRCRHCKARITPRYLLIELLTGALFVACYAAFGL"
BASE COUNT          931 a         1647 c         1329 g          913 t
ORIGIN      
        1 aggcgttcct accccgactt cgcgcggcag attcctgcca caaaactcct tgccaagcca
       61 acggcggcgc aggcaaacaa gggattcttc gctacgctca gaatgacaat cgcaaaggga
      121 tcgtaacagg gtcgcaccag cgctcagcat cccccgcttg cctccgccaa ttcttccggt
      181 gtgttcaaat tccgaaagat cctcggcgca aacccggcca cttccagttc ctcttcccca
      241 atcacctgtg ttcgctgcgc atcgaacagc gcgtcgatct tgtatcgccc cgcgcgcagc
      301 gcgctctcgg cgacatcgcc aaactcccgc cggtaaacag cgcacagcgg ctgccatcct
      361 ccgccggctc gcgccacggt caccgtcgcc aacgaacccc gcgcccgcgt gatcaagtat
      421 tgcaacagcg ccagcgacac aaacggcaca tccacggcca gcaccaggtt cagctcctcc
      481 tgcgacgcgc gcagcgcggc atgaatcccc ccgagcggcc cgcagtcgcg aaacacatct
      541 tcgaccaccg gcgcgaatgc cccaaatttc gctgcatcac ccacaatgcg gacgtcgccg
      601 gtcaccgacc gcgccagctc cagcgcccgc gccagtaagg tccgcccgtc cagggcgacg
      661 aacgccttgt ccgcgcccat gcgcgtgctc ttgccgccgg ccaggataaa aaccgtcaca
      721 tccggcgcca tgccgtccat gcggcgatca tacacggacc gggtgtgatt ccactcaagc
      781 gcgctccctc ggtagtgccc gttgaacttc gatagcccgt gcccctcggt ggcgtcatcc
      841 tgagcgaagc cgcttttcag gcgcagcgaa ggatctcgcg aggagtgcct tggtggcccg
      901 ggagatccct cgcgccgctg gtgcgagcac ggcgcttcgg gatgacatcc gggggaagct
      961 tctcgaatgt cggacctact actcgcctcc ccgtgcccta accctctgca tccgttgaat
     1021 ttatttcctc gctcgtcttt ctactgcacg aaagtaacct acccaaaagc gaaccgtcgc
     1081 gcgatgatgt ctctgcgtcc ggctgaactg acccggaagt tacccaccat gccgcagaaa
     1141 attatggatg tgcttatgct tgggcgctgc tcccacgagt tctcctggcc gcgccgtggc
     1201 gccgacggcg actattacca ggtctgcctg ctttgcgccg cggaatacaa gtacgactgg
     1261 accaccatgc gccgcaccga gcgcgtggaa cacaagcccg aatcgggaag ccgccgcggc
     1321 catacccgcg agaagcggcc gtcctgggta ccacgtgcgc gccgtctgaa actcgatacg
     1381 ccattacgtt atcgcgtgaa ccgcaccagc gcctggcatg aaggcatcat cgaaaacctc
     1441 agccagagcg gcgtgctctt ccacggcccg cagcagctgc cggtcaacgc cctcattgag
     1501 atggtgtttg agatgccgga agaaatctcc ggccagaaga actcgaacgt cgtctgccag
     1561 ggccgagtga tgcgcgccaa agaccaggga gaaaccgaag aaggccacgt cagcctcgcc
     1621 gcctccatcg tggactacaa gttcatccac tgaacggccc gggttaccgt ttgtcggacg
     1681 cggccatctt ttttgattgc aaggaagcgc accttctgtg ttcctacctc agcagcccgc
     1741 ataggctaaa ctgtacccat acacccggct tttgcagccc gtgtcaggag tcccctatga
     1801 aaagagctcc cctcatcatc tcttcctgtg ttcttgcgat catcctcgct ataaccgtgg
     1861 gctgcgccaa aaagcccgac gacgccaaga tttcctccga cgtgcaaggc cggttcagcc
     1921 aggattccgg cctctccacc aagcagctca ccgtgcaatc ggccaacggc gtggtcacga
     1981 tcagcggcac cgtcgataac gatgcccagc gcgatgccgc cgcccggcag gcggccgccg
     2041 tcgccggtgt gaaagaagtc gtcaacaacc tgcaggtggc cggtgctgcg ccagcccctc
     2101 cggccgcagc gcctgccccg gaagcagccc cggctcaaac ggcgaccgcc ccccccgccg
     2161 agaagcctaa accggctccc ggcaagaagt ctcaccgatc gcacgcctcg cagaacgccg
     2221 acaatggaga caattccgcg gcttcgcaga tggccggcaa tcaggctccg cctcctacgg
     2281 ctcaggctgc ggctccgcaa gacacggcca tggccccgcc gcctcctccg cctccgccgc
     2341 caaaaaagtt catcatcgat cagggaaccc agattaccgt gcgcctgatc gatcctatcg
     2401 acagcgagaa gaaccagacc ggagacacct ttcacgccac gttgaacgca ccgctcacct
     2461 ctgatggaga agaagctgtg cccgcaggcg tggaagttac cggtcacttg gtggaagtga
     2521 agagtgccgg caagttcgcc gggcaatcca ccgtggcgat gcaactcgac agcatcgcct
     2581 cgggcggcca gacctacaac gtgcagagcg acatctaccg caagagcggc agctcgcgcg
     2641 gcaagaatac ggctgaaaaa gtcggcggag gcgcactggt tggcaccctt atcggagccc
     2701 tcgccggcgg cgggaaaggc gcggccatcg gcgcggccgc tggtgcgggc gttggtggtg
     2761 gcgtgcaggc ggcaaccaag gcccagcaga tcaagtttcc ttcggagacc gtgctgacct
     2821 tcaccctgca ggcgccggtg accgtgatcc aggcccccaa gccggattcc aaccgccaga
     2881 agctgagtac ctcgcagtag gcgctgggtg gggaacctgt ttcagggaga gccgcggacc
     2941 ggctctccct gatccctctg caaaacttca ttgccgtcgc aagatgcggg cggcctctcg
     3001 tctcgtagca cgcccaggtt tcgcgtacac tctcctccca ggcgtccatc tcatgccttc
     3061 ggtcaccgcc ttgctgcata cccaaaacga tgctttgcgc ctgggccgct gcctggaaac
     3121 gctctatccc tgcgacgaga tcgtgatcat cgaccacgct tcgcgcgatc ttaccgtccg
     3181 catcgcccgg gaatatgggg cgcaggtggt acggcctgtg ccaggggctt ctcccaattt
     3241 ctatgttccg ccttccgatt caacctggct gctctgcctc gctccctgcg aatcccttag
     3301 cgagggcctt gccgcaagcc tgttcgagtg gaaggagctt ccttctttgc cctcggctgc
     3361 ggcgttttca gtgctcgtgc gtgacgaggc cgtcagcgga tgggccgaca tcgccgccct
     3421 ccaaacccgg cttgtgtcct cccgctggaa gcgatgggag ggaacacttc ccacaaacga
     3481 cccttcggcc gccgtcctcg aaggagaact cttgcacttc gttctgcctt gagagtacgc
     3541 ttttgccgac taggagctcg acgacagctt gtgattttga tcacatccgt cgtttcgcct
     3601 cgcgcctaga ctcgcctgtt gtggtctttc gggatcggta acgccctagg aggaagacaa
     3661 tgcgtgtaat gaaattcatc agcctggttt tagtggtcat gacgatttcc gctcttgccg
     3721 tggcgcagca ggccccggct caaactccac ccgaagtaaa gcatgttccc attacgagca
     3781 ctccttcgaa ttccggcaag gagatgttcg aaagctactg cgcggtctgc catggcaagg
     3841 atggcaaggg taacggcccg gcagcctcgg ccatgaagac tccgcccagc gacctcaccg
     3901 gtttggcgaa gaaggaaggc gggaagtatc cctcagctca cgtggcggcc gtgattcgcg
     3961 gacaggccac cacaccgtcc cacggcagcc aggaaatgcc ggtctgggga cccctgttct
     4021 ccagcattag ccagggccac gaggcgcagg tgcagcagag agtcacaaac ctggtgaaat
     4081 acatcgagac tttgcaggcg aagtagagag caaccgtagt ctttgcgaaa tcgacagggg
     4141 acgcacccgt gtgcgtcccc tgaatcctgg agccggattg acctcaagcg gaagtccgcc
     4201 taactgtttt cctcggcgtt cttcgcggat ttccctttgc gtcctctgcg gtaatttgct
     4261 cttcgtgact ccttcagctc ccccgcaagt caacagctcg ccgggaacct cccccgccac
     4321 ccttgccctt caggcataac tacctccgtc tcaatgcgtt ccagctcatt ggccgcttgg
     4381 acagtgactt cagtacctta gtgctcgccc cgatgatcgc cttataatcc atccaagatc
     4441 agcaaataat tgcgttctcc agtgggacgt tcgggctgaa cccgcaggcg tccgcctgcg
     4501 agagcgctgc aggtcaagat catagaagat caagtggatc cagtttttac agccgcgatt
     4561 tttgtcactg ggctggcctt cggcagcttt ttgaacgtgt gtatctaccg cctcccgtta
     4621 gggatatcgg tcgtctcgcc gcgctcggcc tgccccaggt gcaagcacgg cattgccctc
     4681 tacgacaata ttccggtttt gagctggctc gttctccgcg gccgctgccg ccactgcaag
     4741 gccaggatca ccccgcgcta cctgctgatt gagctgctca ccggcgcgct cttcgtggcc
     4801 tgctatgccg ctttcggact
//
LOCUS       DASPUL010000154         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_161489, whole genome shotgun sequence.
ACCESSION   DASPUL010000154 DASPUL010000000
VERSION     DASPUL010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161489"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VEJ00_03765"
     CDS             complement(<1..720)
                     /locus_tag="VEJ00_03765"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX00308.1"
                     /translation="MRAILGQRPLRFVFAANMVSMFGSGMNSAAVAWYILQATHNEMA
                     LGMLVVLQTVPAMMLLPFSGVVIDREDRRRLVMWLDALRGLIILTVAILAFTHRVHVW
                     QLYLMNTLVAAGFWMFWPTITALIQELTAQGQFVQSNTFLMAGVQGGWLMAGAVVGFM
                     YNHVGLGGVLLIDVSTYVVSFLCYLAVRKGRHIVIHPDELRADIVAAENALQRFRRDM
                     REALHFLRDQRAVVLLGASWAL"
     gene            979..2124
                     /locus_tag="VEJ00_03770"
     CDS             979..2124
                     /locus_tag="VEJ00_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00309.1"
                     /translation="MRRCIGLLLVVLFLLCPLFTGKAGGQVDRIVIAAGTDEDHALQA
                     ISNEQDDKKKLAMYEDFVQKFASNPAAVAYGNWQISQAYQTAGDLQKALDYGDKALAG
                     SPHNLDIIVSQANVAQQAKNNAKLMDYAAKGGEVCNSIAKQPKPEGVSDEEFAKRIAE
                     EKSAAQNNCDFLESSGFNAITAENDPKARMAEIEKYTATFPDSKFQDQVSSYALYTLG
                     PGQLNDPARLVAFGEKTLAANPNSLPALLLLANYYSDKPGGAAKCVSYAQKAIEVAKP
                     DAPDADKSRKISAGVAHNSIGWSYLNQEKTSTAIPELKAAAALLKGQDDQQYARALYG
                     LGFAYGKLNKLTEAREVLTEAVKIQGPLQSVSQDLLNKVNSARAKGN"
     gene            complement(2181..2906)
                     /locus_tag="VEJ00_03775"
     CDS             complement(2181..2906)
                     /locus_tag="VEJ00_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00310.1"
                     /translation="MTLALTLLVVVSSVPAAPPVKAPPAQSAAPTTTTVPPQGATKAQ
                     DQGAVPAQNPSPASKSDQASSSAQTSSTQNQPAPPKRPRRKKKPKNVNCVLPPATADS
                     TTTPSDPSARPGAPPAATGSAPADGKSAAATPPFNCPPPKIVVRQGGTTEPSIQLAGG
                     AVGNQAAQQRETANRILKSAEDNLTRIAGRQLSPNQQEMVNQIHQFIEQSRSAIAAGE
                     LERARTLASKAQQLSQDLAQPPQ"
BASE COUNT          646 a          877 c         1015 g          586 t
ORIGIN      
        1 caacgcccag ctggctccga gaagcacgac ggcgcgctgg tcgcgaagga agtgcagggc
       61 ttcgcgcatg tcgcgccgga aacgctggag cgcgttctcg gcggcgacga tatcggcgcg
      121 cagttcgtca ggatgaatca cgatgtggcg gcccttgcgc acggcgagat agcagaggaa
      181 cgagacgacg taagtcgaga cgtcgatcag cagcaccccg ccgaggccaa cgtggttgta
      241 catgaagccg acgacggcgc cggccatgag ccatccgcct tgcacgccgg ccatcaggaa
      301 cgtgttcgat tgcacgaact ggccctgggc cgtgagctcc tgaatcagcg cggtgatcgt
      361 gggccagaac atccagaagc cggcagccac gagcgtgttc atcaggtaga gctgccagac
      421 atgcacgcga tgcgtgaaag cgaggatggc gacggtcagg ataatcaggc cgcgcagcgc
      481 gtcgagccac atgaccaggc ggcggcgatc ctcgcggtcg atgaccactc cggaaaacgg
      541 caacagcatc atcgcgggga cggtctgcag aaccacgagc atccccagcg ccatttcgtt
      601 atgtgtcgcc tgaaggatgt accaggcgac ggcggcagag ttcatcccac tgccgaacat
      661 ggaaaccatg ttggcggcaa agacgaatcg cagcggacgc tggcccagga tggcgcgcat
      721 cgttctactt tagccgagga gcctgtgaat tcaccacgga ggcaggagaa gcaatgcttg
      781 attgatgatt ttcgattgta ggtaggactg cggcaccggc cccgtgggat ttcaatcggc
      841 aatcttcttg ggcctctggg cctccgtgct aagaatgtct tcgcccagtg attccgccgc
      901 ttgcgtgcag ccgcgttcgg cgctaggatt cgcgtgctgc ctgaactaga ggtcgtttcc
      961 cggcaccgga ggtagcccat gagacgctgc atcggtttgc tgctggtggt tcttttcctg
     1021 ttgtgtccgc tgttcacggg aaaggcgggc ggacaggtgg accgcattgt gatcgcggcc
     1081 ggaacggacg aggatcacgc cttgcaggcc atctccaacg agcaggacga caaaaagaag
     1141 ctggccatgt acgaagactt cgtgcagaag ttcgcgtcga acccggctgc cgtagcctat
     1201 gggaactggc agatctcgca ggcctatcag accgccggcg atctgcagaa ggctttggac
     1261 tatggcgaca aggcgctggc cggttcgccc cacaatctcg acatcatcgt atcgcaggcc
     1321 aatgtggccc agcaggccaa gaataatgcc aagctcatgg actacgccgc taaaggcggc
     1381 gaagtgtgca actcgatcgc gaagcagccc aagcccgaag gggtcagcga cgaggagttt
     1441 gccaaacgca tcgccgaaga gaaatcagcc gcgcaaaaca actgcgactt tctggagagc
     1501 tcgggattca acgccattac cgcagaaaat gatcccaagg cgcgcatggc cgagatcgag
     1561 aagtacacgg ccaccttccc cgattccaaa ttccaggacc aggtttcgag ctatgccctg
     1621 tatacgctgg gcccgggaca actgaatgat cccgcgcggc tggtggcgtt tggcgagaag
     1681 acgttggcgg ccaatccgaa ctcgctgccg gcactactgc tgctggccaa ctattacagc
     1741 gacaagccag gcggtgcggc caagtgcgtc tcatacgcgc aaaaggcgat tgaggtcgct
     1801 aaacctgacg ctccggacgc cgacaaatcg cgcaagattt ctgcaggagt ggcgcacaac
     1861 agcatcggct ggtcttacct gaatcaggag aagacgtcca ctgccattcc ggagctcaaa
     1921 gccgcagccg ctttgctcaa agggcaggac gatcaacagt atgcccgagc attgtatggt
     1981 ctgggctttg cctacgggaa actgaacaaa ttgactgagg cgcgcgaagt gttgaccgag
     2041 gccgtcaaga ttcagggtcc gctgcagagc gtttcacaag atttgctgaa caaagtgaat
     2101 tcggcccgcg ccaagggaaa ctgagccggc agcagtttcg caatacgcgg cagacgaggg
     2161 tgcttctgtc aggactctga ctactgcgga ggctgggcga ggtcctggga gagctgttgc
     2221 gcctttgagg ccaaagtgcg tgcgcgctca agctctcccg cggcaattgc gcttctggac
     2281 tgctcgataa actggtgaat ctgattcacc atctcctgct ggttggggct gagctggcgt
     2341 cctgcgattc ttgtgagatt gtcttctgca gatttcagga ttcggttggc ggtctctcgc
     2401 tgctgagcgg cctggttgcc aactgcgcct ccggccaact gaatgcttgg ctcggtggtt
     2461 cctccctggc ggacaacaat cttcggtggc gggcagttga agggaggagt cgcggctgca
     2521 gactttccat ctgcgggcgc agagccggta gcggccggcg gcgctcccgg ccgggcagaa
     2581 ggatcagacg gcgtcgtcgt cgaatcggcc gttgctggag ggagcacgca gttgacgttt
     2641 tttggcttct tcttgcgacg tgggcgtttg ggaggcgcgg gctgattctg ggttgacgaa
     2701 gtctgggcag acgaagaagc ttgatcggac ttggaagcag gagagggatt ctgggcagga
     2761 actgctcctt gatcctgagc tttcgtggcg ccctgcggcg gaacagtcgt ggttgttggg
     2821 gctgcagatt gcgcgggagg cgccttcacc ggtggcgcag ccggtacgct ggaaaccacg
     2881 acgagtagcg ttagggccag ggtcatgcgc ggatctggag ttcagcgccc agaggttaat
     2941 cttacgcctt tcaagggcga agcaggagag ttgatggtct ccgaggccat ccccacgagc
     3001 gccccggggt tatgaggctg ccacgtcggc ggcataggcg tgaccagcct cccgcgccgg
     3061 tcctgccacc ggaatatgga agcggaaggt tgtgccgacg ccttcggttg attcaaaatc
     3121 cacc
//
LOCUS       DASPUL010000155         3598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_983743, whole genome shotgun sequence.
ACCESSION   DASPUL010000155 DASPUL010000000
VERSION     DASPUL010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983743"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VEJ00_03780"
     CDS             <1..1176
                     /locus_tag="VEJ00_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00311.1"
                     /translation="TRGLPAMVPVAMLYEIPENSAAEIAYIEKRGYPISYVEMGEEAD
                     GQYMLPEDYAALYLQWATALHKVDPKLKLGGPSFQGVNRDIEVWPDANGKASWLGRFV
                     DYLKQHGRMNDLAFFSFEHYPMEPCRIPWGMLYDEPELVSHIMHVWHDDGIPADVPMF
                     ITEGNLSSAASETYQDIFSGIWLADYVGSFLNAGGKGLYFFHYLPLPMEPGCNASPGT
                     FGMFTINNNYEIQQPLAQYFVAQLVNTEWVQPSGGEHQVYAAKSDISDGAGHDLITAY
                     ALKRPDGQWAVMLVNRDQQAAHKVRIAFEDHSTAGFAGPVEILTFGSAQYQWHPAATR
                     FMAHAEKAGERTIVATTKGWADPDGPIARSKQNAGNDTLFELPAASVSVIRGAIASR"
     gene            complement(1239..>3598)
                     /gene="priA"
                     /locus_tag="VEJ00_03785"
     CDS             complement(1239..>3598)
                     /gene="priA"
                     /locus_tag="VEJ00_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXX00312.1"
                     /translation="LVPFRQQRMTGIVVELHDRTPSVTAKNIVGVLDSAPVLDEQLLR
                     LGRWIADYYLAPLGEVFRTMLPLSAEFRRSIGYRITEQGQMALHLAGMAGSSARSQRT
                     PEEQAAEFRVLDYLAGHEADLGGAELVREQTLRAATQASRRVLTGMVRKKWVVREDVS
                     GPQVAARTVKIGVLNSADGKLNDNQRTLIDTLAASGGRVSVEALRSLAVPRTTLGTLV
                     RRGLVKIVEEPAEFTVSRTKPRPSPFDFDFNGAQQSALARLRDGVAARKFSGMLLHGV
                     TGSGKTAVYLAAMRAVLEAGRSAILLVPEIGLTPAVAADLHQIFGDEVAILHSALSDR
                     ERAEQWHRIKAGEARVVVGTRSAVFAPVSDLALIIVDEEHDSSYKQEETPRYHARDVA
                     VMRAKMAGAVVVLGSATPSLESYFNATKNKYALVELPDRVEQRPLPEVEIVDMRQEFQ
                     ETGHEQVISRKLAAEIKERLERKEQVMVLLNRRGYSPVVLCRTCGKKLECKNCAIALT
                     HHKREHKMVCHYCGYMAAVPKTCVYCGSEYVYFLGTGSEKLEELLHGIFPQARIARLD
                     RDTVRSHEDFERTLNALSERELDVVVGTQMIAKGHDIHGVTLVGVVGADVALGLPDFR
                     AAERTFQLLTQVAGRAGRGESPGKVILQTYFQDHYAVQYAARHDFVGFYEKELRFRSW
                     MHYPPYSALANVLVRSDKLDEALRWSGTLGKWFESTRHEGVRVLGPATAPIMRLKRDY
                     RYHFVLKSPSREKLNGILRAMLVYAAQQKIPRTQVIVDVDALWLM"
BASE COUNT          717 a         1185 c         1028 g          668 t
ORIGIN      
        1 acgcgcggac ttcccgcgat ggtcccggtc gccatgctct acgagatccc cgaaaattcc
       61 gccgccgaga tcgcctacat cgagaaacgc ggttatccga tctcctacgt cgagatgggc
      121 gaagaagccg acggccagta catgctcccg gaagactacg ccgcgctgta cctgcagtgg
      181 gccacggcgc tgcataaagt cgaccccaaa ctgaagctgg gcgggccgtc gtttcagggc
      241 gtcaaccgcg acatcgaagt ctggcccgac gcgaacggga aggcctcctg gctcggccgc
      301 tttgtcgact atctgaaaca acacgggcgc atgaacgacc tcgcgttctt ctccttcgaa
      361 cactatccca tggagccctg ccgcattcct tggggcatgc tttacgacga gcccgagctg
      421 gtcagccaca tcatgcatgt ctggcacgac gacggcatcc ccgccgacgt gcccatgttt
      481 atcaccgaag gaaatctgtc gtcagcagcc agcgagacct atcaggacat cttctccggc
      541 atctggttgg cagattatgt cggatcattc ctgaacgccg gcggcaaggg gctgtatttc
      601 ttccactacc tgccgctgcc gatggagccg ggctgcaacg cgtctccggg gacattcggc
      661 atgtttacca tcaacaacaa ctacgagatt cagcagccgc tggcgcagta cttcgtggcg
      721 cagctggtca acacagaatg ggtgcagccg agtgggggtg agcaccaggt gtacgcggcg
      781 aagagcgata tctcagatgg cgcgggtcat gatttgatca cagcctacgc gctgaagcgc
      841 ccggacggcc aatgggctgt gatgctggtg aaccgcgatc agcaggccgc acacaaagtg
      901 cgtatcgcat tcgaagacca ctcgacggcg ggtttcgccg gacctgtcga gattctgacc
      961 ttcggtagcg cccagtacca gtggcacccg gcggcgacgc gcttcatggc gcacgccgaa
     1021 aaggcaggcg agcgcaccat cgttgccacc accaagggtt gggccgatcc cgatggcccg
     1081 atcgcgcggt caaagcagaa tgcaggcaac gacacgttgt tcgaattgcc ggccgcgtcg
     1141 gtctctgtga taagaggcgc catcgcctcc cgctagttcg tggagcagat ggattttgga
     1201 aagctaagag cgaagagcca atcgccgaaa gccgcgtttc acatcagcca cagcgcatcc
     1261 acgtccacaa tgacctgcgt gcgcgggatc ttctgctgcg ccgcgtaaac gagcatcgcc
     1321 cgcagtatgc cgttgagttt ttcccggctc ggcgacttca ggacaaagtg ataccggtag
     1381 tcgcgcttca accgcatgat gggagccgtc gccggtccca gcacgcgcac gccttcgtgc
     1441 cgcgtcgact caaaccactt ccccagcgtt ccggaccatc gcaacgcctc gtccagcttg
     1501 tcactccgca ccagcacatt cgccagcgcg gaatacggcg gatagtgcat ccagctgcgg
     1561 aaccgaagtt ccttttcata gaaaccgacg aaatcatggc gcgcagcgta ctgcacggcg
     1621 tagtgatcct gaaagtaagt ctgaagaatg accttgcccg gcgattcgcc ccggccggcg
     1681 cggcccgcga cctgggtcag cagctggaaa gttcgctctg cggcgcgaaa gtccggcagg
     1741 cccagggcaa cgtctgcgcc caccacgcca accagcgtca cgccatggat gtcgtgccct
     1801 ttggcgatca tctgcgtgcc gacaaccaca tccagctcgc gctcgctcaa tgcgttcaag
     1861 gtgcgctcaa agtcctcatg gctgcgaacc gtgtcgcgat cgagccgcgc aatccgtgcc
     1921 tgcgggaata tcccatgcag aagttcctcc agcttttccg atcctgttcc cagaaaataa
     1981 acgtattcgc ttccgcaata gacgcaggtt ttcggcaccg cagccatgta gccgcaataa
     2041 tggcagacca tcttgtgctc gcgcttgtga tgcgtcaggg caatcgcgca gtttttgcat
     2101 tccagcttct tgccgcaagt gcggcagagc acgaccggcg aatatccacg ccggttgagt
     2161 agcaccatga cctgctcttt gcgctcgagc cgctccttga tctcggccgc cagcttgcgc
     2221 gagatcactt gctcatggcc ggtttcctga aactcctgcc gcatgtcgac gatctcgacc
     2281 tccggcagcg gccgctgctc cacgcgatcc ggcagttcga cgagcgcgta cttgttcttt
     2341 gtcgcgttga aataagactc gagagacggc gttgccgagc caagcacaac cacagcaccg
     2401 gccatcttgg cgcgcatcac ggccacatcg cgcgcgtgat accgcggcgt ttcttcctgt
     2461 ttataggatg agtcgtgctc ctcatcgacg atgatcagag ctaaatcgga taccggtgca
     2521 aacaccgccg atcgcgtgcc caccaccacc cgcgcttccc cggccttgat gcgatgccac
     2581 tgctcggcgc gctcgcggtc cgagagagca gagtgcaaaa tggcgacttc atcgccaaag
     2641 atctgatgca ggtcggcggc gacggccggt gtgagcccga tctccggaac gagcaggatt
     2701 gccgaccgtc cggcttcgag caccgcccgc attgcggcca gatacacggc agtcttcccc
     2761 gacccggtca cgccatgcag aagcatgccg gaaaattttc gcgccgcgac gccatcgcgc
     2821 agacgcgcaa gcgccgactg ctgcgcaccg ttgaaatcaa agtcgaatgg cgacggccgc
     2881 ggtttcgtac gcgaaaccgt aaattccgca ggttcctcga cgatcttgac cagcccacgc
     2941 cgcaccagtg tgcctagcgt cgtgcgcggc accgccagcg agcgtagcgc ttccacggag
     3001 actctgcccc cggacgcagc cagcgtgtcg atcagcgtcc gctggttgtc attcagtttg
     3061 ccgtcagccg aattgaggac accgattttc accgtccgcg cggcaacctg tggcccggaa
     3121 acgtcttcac gcaccaccca ttttttccgg accatccccg tgaggacgcg cctcgatgcc
     3181 tgggtggccg cacgcagtgt ctgctcccgc acaagttccg cgcctccgag gtcagcctcg
     3241 tgcccagcca gatagtcgag cacgcgaaac tccgcggcct gttcctccgg cgtacgctgc
     3301 gatcgcgcgg acgatcccgc catgcccgcc agatgcagcg ccatctgccc ttgctccgtg
     3361 atccgatagc cgatggagcg tctgaactcg gcgcttaacg gcagcatggt ccggaagact
     3421 tcacccaatg gagcaaggta gtagtcggcg atccagcgcc cgagccgcag cagttgttcg
     3481 tcgagcaccg gagcggaatc gagcactccg acgatgttct ttgccgtaac ggacggcgta
     3541 cggtcgtgca actcgacaac aatgcccgtc atcctttgct gtcgaaaggg aaccaaca
//
LOCUS       DASPUL010000156         6256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_301851, whole genome shotgun sequence.
ACCESSION   DASPUL010000156 DASPUL010000000
VERSION     DASPUL010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301851"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..427)
                     /locus_tag="VEJ00_03790"
     CDS             complement(23..427)
                     /locus_tag="VEJ00_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXX00313.1"
                     /translation="MTSAKVIWTDNERYVGEADSRHAIVMDTAAQKTASTPMELVLIA
                     LCGCTASDVVGILRKKREPFTHLEVRAEGERAKGYPAVYTEIKLVYRIGGKVSRKAVE
                     DAVRLSEEKYCSVSAMLQKTAKITFEIEILDA"
     gene            complement(433..1863)
                     /locus_tag="VEJ00_03795"
     CDS             complement(433..1863)
                     /locus_tag="VEJ00_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HXX00314.1"
                     /translation="MLGTPATLSPEAMRADKRAAAGNSVVAAVVITTLKVVVGISTGS
                     LGILSEAAHSGLDLIAALVTFFSVGVSDKPADADHQYGHGKVESFSAFLETGLLLLTC
                     AWIVYEAGVRLFLRRVEIEPSIAAFAVMLISMGVDWWRSRVLGRIAVKYDSQALEADA
                     LHFSTDIWSSGVVLLGLLLVMAGRAYRIDWLRDADPVAALFVAGVVVSVSWRLARRTV
                     DVLLDAAPPGVRSQIQDAVSRVDGVLEVDRVRIRRAGNRYFADVAVGLARTVTFQRSG
                     QLAAAVTEAVRRVLPDADVTVEPLPRAERSENTFDRIRAVATHRNLNVHDISVQDLAG
                     RLHVEQHIELDERMTLKDAHDRVTELETDLLREVPEISDILTHIESEPATIEQSEEVV
                     SDAQLEQRLKKVAAQVPEILDVHEFVVKRVRGRLYISCHCTLSDDLPLARVHDIQTDL
                     EIRFKQDAPDLFRVLIHPEPSTDNRR"
     gene            1978..3159
                     /locus_tag="VEJ00_03800"
     CDS             1978..3159
                     /locus_tag="VEJ00_03800"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXX00315.1"
                     /translation="MGKSWLLCALLGALAWGQGGPEPASPPKAAQSPSDASAALADDT
                     PVITVEGVCAPQPKATTAKGATTATKPATSAKPAASDCKTVITKAQFEKLADALSPTP
                     LNPQQKKALAARLAQIIPMANAATKKGLDKSPKYAETLKYAKMQILAGEMITSIQQEA
                     AKVPQEDIENYYKKNPEAFEQFVLERLYVPRTKQVEPETKEVELKDENSAKLTPEQEK
                     AKQAEAKEKAEQAEQDMTKLADSLRTRAAGGEDFVKLQKEAYDAAGIKIESPTVTLPK
                     MRRTGLPPAQASVFDLKVGEVSPVISDSGGHYIYKVDSKDAMPLDQAQTEIHNRLQGD
                     RSREMMEKVTGSVKTVQNEAYFGGAAQSMMGQRPMPPQRVPGNPPAAQQQTPPPAPKP
                     N"
     gene            3160..4236
                     /locus_tag="VEJ00_03805"
     CDS             3160..4236
                     /locus_tag="VEJ00_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXX00316.1"
                     /translation="MAKPVVVVTGVSGNLGSRLLSLLGSYSVIGVDVGAPRSDAELRF
                     EALDLGQESSTRTLYELLRDTHPYAVVHLAFVIDPVRTGVLDVERMWQINVAGTARVM
                     EAITEVNRASDDGIKQFIFPSSVSAYGPNLPTAATEESPLAAHTLPYAVHKKQSDEVV
                     QQRSPALRGCSVYILRPHIFAGASVENYLMGAFKGTPNGKGERARKMRERGTRLPCML
                     PTGQKYLDNKIQFVHVDDMARLIQHILQREPETQRLTVLNVAGRGEPLTFARCIEMAQ
                     AKLVRVPGKWAMRQILKFLWKRQISAIPPDALPYMTGEYVMNTDRLRRFLGPEYEHVM
                     RYTIADAFADSFKPASSSQSASAG"
     gene            complement(4310..4837)
                     /locus_tag="VEJ00_03810"
     CDS             complement(4310..4837)
                     /locus_tag="VEJ00_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXX00317.1"
                     /translation="MPLRLRRRAMAPRLAVVAAAGLLFAGCKVRTPGKFETGFMNGTK
                     RRLTVGGRSDVNPIPASAENIRMGQQNFTAYCMVCHGLDGQNTGVPFAEKMAPPVPPL
                     NSAAVQAYSDGQLHWIIKNGIFPSGMPAAKDLFHDDEIWQMVLYIRHLPPKGSLGEPQ
                     VYGGAPPEPAKKTVP"
     gene            complement(4840..5211)
                     /locus_tag="VEJ00_03815"
     CDS             complement(4840..5211)
                     /locus_tag="VEJ00_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HXX00318.1"
                     /translation="MTRIKLPRTIVATALCLIGLLSLTGARRAAADDLPVVEISAKRF
                     AFTPDKITLKKGQTVKLRLHSEDVTHGFFLRPLKIDEEIPAGQTVEVTVTPQVTGSFM
                     TICDHFCGANHGNMNMTIVVE"
     gene            complement(5258..>6256)
                     /locus_tag="VEJ00_03820"
     CDS             complement(5258..>6256)
                     /locus_tag="VEJ00_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXX00319.1"
                     /translation="MKQTDRRSFLKIAGTSLGIGVLYSAYPAAMAKGQVGDMFASLGR
                     ANGEKVEPFSFIQLSDAHVGFNGPPDPLGTKAFERAVDMINALPKRPDLVLFTGDLTH
                     DTEDKDVHSQRMKQFQEISKRINVPLIKCVPGEHDAGLDAGALYREFFGESHYSFDHR
                     GVHFVALDNVSRARPEVGTEQLEWLKKDLARFPKTAPIVVFTHRPLFDLRPEWEWFTS
                     DGDDVMNVLSAYDNLTVLYGHIHRDDEHETGKVHHYAARSLIFAFPDPNSGPGPRKPI
                     PFDKDHPFKNLGIRTVEFNGKPRPGSSPLSIEDVELTAQEFRGINGAHQFLKNPNS"
BASE COUNT         1278 a         1888 c         1930 g         1160 t
ORIGIN      
        1 gaggcgaact ccggttgcgc gactacgcgt ccaggatttc gatttcgaat gtaatcttcg
       61 cggtcttctg cagcatggcg gacacggaac aatatttctc ttccgagagc cggaccgcgt
      121 cctccacggc cttgcgagac actttaccac cgattctgta aaccagtttg atctcggtgt
      181 aaaccgcggg atatcccttg gcgcgctcac cctcggcgcg cacttcgaga tgagtaaagg
      241 gttcgcgttt cttgcgcaga atgccgacga cgtcggaggc tgtgcagccg cagagcgcga
      301 tcagcaccag ttccatggga gtagaggcgg tcttctgcgc ggccgtgtcc atgacgatgg
      361 cgtggcggga atcggcctct ccgacgtagc gctcgttatc cgtccagatg actttggcag
      421 atgtcatgtt gtctatctcc gattatccgt gctcggctcg ggatgaatta acacgcgaaa
      481 cagatcaggc gcatcctgct tgaagcggat ttccaggtca gtctgaatat cgtgcacacg
      541 cgcgagcggg agatcgtcgg acagagtgca gtggcaggat atgtacagcc tcccgcgcac
      601 tcgcttgacc acgaactcgt gtacgtcgag gatctcggga acctgcgccg ccactttttt
      661 caaacgctgc tccagctgcg cgtcgctcac cacctcttcc gattgctcga tcgtggcggg
      721 ctcgctctcg atgtgcgtca ggatgtcgga aatttcaggg acctcgcgca gcagatcggt
      781 ttcgagttca gtgactcgat cgtgagcgtc tttcagcgtc atgcgctcgt cgagttcgat
      841 gtgctgctcg acatgcaggc ggcctgcgag atcctgcacg ctgatgtcat gcacgttgag
      901 gtttctgtgc gtggccacgg cgcgaattcg gtcgaaggta ttctccgagc gctcggcccg
      961 aggcaacggc tcgacggtca cgtcggcatc gggcaaaacc cggcgaactg cttcggttac
     1021 agctgccgcg agctgccctg aacgctgaaa cgtcaccgtg cgcgccagac cgaccgccac
     1081 gtcggcgaaa tagcggttgc cggcgcggcg gatgcgcacc cggtcaacct caagaacgcc
     1141 gtcgacccgc gagacagcgt cctgaatctg agagcgcacg ccaggaggcg cggcatcgag
     1201 caggacgtca accgtccggc gggccaggcg ccagctcact gacacgacca cgccggcgac
     1261 gaagagggcc gcaaccggat cggcgtcgcg cagccagtcg atgcgatagg cgcgcccggc
     1321 catgaccaga agcaagccca gcagcaccac accggaggac cagatgtcgg tcgagaagtg
     1381 cagagcgtcg gcctcgaggg cctggctatc gtatttgaca gcgatgcggc ccagtacgcg
     1441 cgagcgccac cagtcgactc ccatggagat cagcatcacg gcaaaagccg cgatggaagg
     1501 ctcgatctcg acgcgccgca agaacaggcg cacaccggcc tcgtagacga tccaggcaca
     1561 ggtgagcaaa agcaggcctg tctcgaggaa cgcggaaaag ctctcgacct tgccgtggcc
     1621 gtactggtgg tcggcgtcgg ccggcttgtc ggaaacgccg acggagaaga acgtgaccag
     1681 cgcggcgatc agatcgaggc ccgaatgcgc cgcttccgag agaataccga gactgccggt
     1741 ggagatgccg actacgactt tcagggtggt gatgacgacc gcggcgacta cggagtttcc
     1801 cgcggcggcg cgcttgtcgg cgcgcatcgc ttccggtgaa agcgtggcag gagttcccag
     1861 cattgaggca attatcgccg ctgccgccgc aaagcgccaa gcagttcgcg cttgtgctag
     1921 gatttcttgt ttagcacttt ccaatttctg atgaaccaat cctggaggat gagggttatg
     1981 gggaagagtt ggctgctgtg tgctttgttg ggagcgttgg cctgggggca aggcgggcct
     2041 gagccagcct caccaccgaa agcggcacag tccccgtccg atgcgtcggc tgcccttgcc
     2101 gacgatacgc ccgtgattac cgtcgaagga gtctgtgcgc ctcagcccaa ggccaccacc
     2161 gccaagggag cgaccaccgc gaccaagccg gcaacgtcgg cgaagcccgc agcttccgat
     2221 tgcaagaccg tgatcaccaa ggcgcaattc gaaaaactgg ccgacgccct gtccccgact
     2281 ccgctcaacc cgcagcagaa gaaagcattg gcagccaggc tcgcccagat catcccgatg
     2341 gccaacgcgg ctacgaaaaa ggggctcgac aaaagtccga aatacgccga gaccctcaaa
     2401 tacgccaaga tgcagatcct ggcaggcgaa atgatcacca gcattcagca ggaggcggcc
     2461 aaagtcccac aagaagatat cgagaactat tacaagaaaa acccggaagc ctttgagcag
     2521 ttcgtgctcg aacgcctgta cgtgcctcgc accaagcagg ttgaacccga aaccaaagag
     2581 gtggaattga aggacgaaaa ctccgcgaag ctgactcctg agcaggagaa agccaagcag
     2641 gcagaagcca aagaaaaagc cgaacaggcc gagcaggaca tgaccaagct ggccgacagc
     2701 ctgcgcacgc gggctgcggg cggagaggat tttgtgaagt tgcagaagga ggcctacgat
     2761 gcggccggca tcaagatcga gtctccgacc gtcaccctgc ccaagatgcg gcgtaccggc
     2821 ttgcctccag ctcaggcctc cgtgttcgat ctgaaggtgg gcgaagtgtc cccggtcatc
     2881 agcgactccg gcgggcacta catctacaaa gtcgacagca aagatgcgat gcccctcgac
     2941 caggcgcaga ctgagatcca taaccggctg cagggcgatc gctcgcgcga gatgatggaa
     3001 aaggtgacgg gctcggtcaa gaccgtccaa aacgaagcat acttcggcgg agcggcccag
     3061 agcatgatgg ggcagcgtcc gatgccgcca cagcgggttc cgggcaatcc tcctgccgcg
     3121 caacagcaaa cgccgcctcc ggcgccgaaa ccgaactaaa tggccaaacc tgtcgtcgtc
     3181 gtaaccggtg tttcgggcaa cctgggatcc aggctcctgt cgttactggg gagttattcg
     3241 gttatcgggg tagacgtagg cgcgcctcgc agcgacgcgg agttgcggtt tgaggctctg
     3301 gatctggggc aggaatcgtc gacccggacc ctctacgaac tcctgcgcga tacccaccct
     3361 tacgccgtgg tccatctggc tttcgtgatt gatcccgtgc gcacgggggt gctcgacgtc
     3421 gaacgcatgt ggcagatcaa cgtcgccggc acagcgcgag ttatggaggc gatcaccgag
     3481 gtcaaccggg ccagcgacga cgggatcaag cagttcattt ttcccagcag cgtctccgcc
     3541 tacgggccga atctgcccac ggctgcaacc gaggaatcgc cactggccgc tcataccctg
     3601 ccctacgccg tccacaaaaa gcagtcagac gaggtcgtgc agcagcgctc gcccgcgctg
     3661 cgcggttgca gcgtctacat tctgcggccc cacatctttg ccggagccag cgtggagaac
     3721 tacctgatgg gagcgttcaa aggcacgccc aacggcaaag gcgagcgcgc gcggaagatg
     3781 cgcgagcgcg ggacacgcct tccctgcatg ttacccactg ggcaaaagta tctcgacaac
     3841 aagattcaat tcgttcacgt cgacgacatg gcgcggctga ttcagcacat tctgcagcgc
     3901 gagcccgaaa cccagcgcct gaccgtgctg aacgtcgccg gccggggcga gccgctcact
     3961 tttgcgcgct gtatcgagat ggcgcaggcg aagctggtac gcgtccccgg aaaatgggcg
     4021 atgcggcaga tattgaaatt cctctggaag cgccagattt cggccatccc ccccgatgcc
     4081 cttccctaca tgacgggcga atacgtcatg aacaccgacc gtctgcggcg gttccttggc
     4141 cccgaatacg agcacgtgat gcgctacacg atcgccgacg ctttcgccga cagcttcaaa
     4201 ccggcgagca gttcgcaaag cgcgagcgca ggataacgtt cctcccgaag cacgcaagac
     4261 tcttcgtcag cgcgatcatc cagaagaaga gaccgtagct tcactaacct cacgggacag
     4321 tctttttggc cggttcgggt ggcgcgccgc cgtagacttg cggctcgccc agacttccct
     4381 tgggcggcag atggcggatg tagagcacca tctgccagat ctcgtcatcg tggaacaagt
     4441 ctttcgcggc cggcatgcct gaggggaaga ttccattctt gatgatccag tgcagttggc
     4501 cgtcgctgta ggcctggacc gcagcggaat tcaacggcgg caccggcggc gccatcttct
     4561 cggcgaaggg cacgccagta ttctggccgt cgagaccgtg gcagaccatg caataggcgg
     4621 tgaagttctg ctggcccatg cggatgttct cggcgctggc tggaatcggg ttgacgtcag
     4681 agcgcccgcc gacagtcagg cggcgcttcg tgccgttcat gaacccggtc tcgaacttgc
     4741 ccggcgtgcg gactttgcat ccggcgaaca ggaggcccgc cgccgcaacc acagcgagtc
     4801 gcggcgccat ggcgcggcgg cggaggcgga gaggcatagc tactccacca cgatcgtcat
     4861 gttcatgttg ccgtgattgg ctccgcagaa gtgatcgcag atggtcatga acgatcctgt
     4921 cacctggggc gtcacggtta cttccactgt ttggccggcg gggatttctt catcgatctt
     4981 gagcggccgc aggaagaacc cgtgggtcac gtcctcgctg tgcaaacgaa gtttcacggt
     5041 ctgtcccttc tttaacgtga tcttgtccgg cgtaaaggca aagcgctttg cgctgatctc
     5101 caccacgggc agatcatcag ccgctgctcg gcgggcgccg gtcaggctca aaagcccgat
     5161 caggcagagg gcggttgcga ctatcgttct tggcaactta attctggtca taattcctcg
     5221 ttcttgagct ggatccttcc gtatctccat gtagtactta actgtttggg tttttcagaa
     5281 actgatgggc tccattgatg cctcggaact cttgggccgt tagctccacg tcctcgatcg
     5341 agagcggact ggaccctggt cgcggcttgc cattgaattc caccgtgcgg atccccaggt
     5401 tcttgaaggg atgatccttg tcgaagggga tgggcttgcg cgggccgggt ccggagttcg
     5461 ggtcggggaa agcgaaaatc agcgaccgcg ccgcgtagtg atgcaccttg ccggtctcgt
     5521 gctcgtcgtc gcggtggatg tggccgtaga gcacggtgag gttgtcgtag gcagagagga
     5581 cgttcatcac gtcgtcgccg tcgctggtga accattccca ctcggggcgt agatcgaaca
     5641 gcggccgatg ggtgaagacc acgatgggcg cggtcttcgg aaaacgcgcc agatctttct
     5701 tcagccattc cagttgctcg gtgccgactt cgggcctggc gcgcgacacg ttgtcgagag
     5761 cgacaaagtg tacgccccgg tggtcaaacg agtagtgact ctcaccgaag aattcgcgat
     5821 agagagcacc ggcgtcgagt ccggcgtcgt gctcgccggg aacgcactta atcagcggca
     5881 cgttgatcct cttcgaaatc tcctggaact gcttcatacg ctgcgagtgg acgtctttgt
     5941 cctcggtgtc gtgtgtcagg tcgccggtga acaggacaag gtcaggccgc ttcggcaggg
     6001 cgttaatcat gtcaaccgca cgctcgaacg ccttggtgcc gagcggatcc ggtggcccgt
     6061 taaagccgac atgggcgtca ctgagctgaa tgaaggagaa cggctcgact ttttcgccgt
     6121 tggcccggcc gagcgacgca aacatgtctc ccacctggcc tttggccatt gccgccgggt
     6181 aagccgagta gagcacgccg atgcccagcg acgtgccagc aattttcaaa aacgatctgc
     6241 gatcggtttg tttcat
//
LOCUS       DASPUL010000157         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1941983, whole genome shotgun sequence.
ACCESSION   DASPUL010000157 DASPUL010000000
VERSION     DASPUL010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1941983"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VEJ00_03825"
     CDS             complement(<1..413)
                     /locus_tag="VEJ00_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HXX00320.1"
                     /translation="MTRPRSLRPLLLGHRGARAFKSIPENTLASFDQALADGCDGFEF
                     DVRLTADSKAVVCHDPRLGGIEIAEAKARQLTALAKLADVLGRYQERAFLDIELKVAG
                     LEGITAGLLRRYPPHAFVVSSFLPEVLQAVHAVDA"
     gene            complement(421..729)
                     /locus_tag="VEJ00_03830"
     CDS             complement(421..729)
                     /locus_tag="VEJ00_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00321.1"
                     /translation="MPKSPANFEVTCPCCSAVLKIDTATRAVIAHTAAVKPKTFSDME
                     AAAKAMREQDSRRESIFQQSVEAQKHAADLLEKKFQEALKKAKETPDTGKPIRDFDLD
                     "
     gene            complement(838..1200)
                     /locus_tag="VEJ00_03835"
     CDS             complement(838..1200)
                     /locus_tag="VEJ00_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00322.1"
                     /translation="MALPAAMSMLSGFARTTRAALALYPVARIYAGLQAALFPSVAVA
                     LLYRGSRQDVYNILWAVVFGFATLNILSLAARRFEPARRGLTFGELLAVMVVLISMFL
                     LGWEMLNLFHVFPIKLKR"
     gene            1367..2035
                     /locus_tag="VEJ00_03840"
     CDS             1367..2035
                     /locus_tag="VEJ00_03840"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXX00323.1"
                     /translation="MARLRFIFFDVGNTLLFPNRARILAPLPQDRHPNLEHWQALERR
                     TKQEFDRGMMGGKVDHGFWWTFHTYLLDELNLPADGVRDLLVQNTQQSANWDQILPGT
                     RAVLERIGQQYATAVISNADGRIDAVLRRCAIADCFHSITDSGIVGQEKPHPAIFEAA
                     LREMKAEPAESLYVGDVYSVDYVGARNAGMEAVLFDVAGAYRERDLPRVESLDELGTW
                     LLKA"
     gene            2066..2704
                     /locus_tag="VEJ00_03845"
     CDS             2066..2704
                     /locus_tag="VEJ00_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HXX00324.1"
                     /translation="MFDSARFVLFLTAALLLAVAPGPGMLYVLARSLAGGKREGVLSA
                     LGTFLGGMVHVLAAALGISIILAKSAAAFAAVKYLGAAYLCFLGVRMILDVRKERAGV
                     EVIPDLGRAARNPLWQGVATEVLNPKTALFFLSFIPQFVVRGDGHVFLQFVALGTISV
                     VMNTTADLMVIALAGPLGEKIRSSATFRRRQRTVTGAIMIGLGTYLATSESK"
BASE COUNT          559 a          828 c          981 g          591 t
ORIGIN      
        1 ttggcgtcga ccgcatgaac cgcctgcaac acctccggca ggaacgagga aaccacaaat
       61 gcatgcggcg gataccgccg cagcaagcct gccgtgattc cttccaggcc ggcgactttc
      121 agctcaatgt cgagaaacgc ccgttcctga tagcggccga gcacgtccgc caatttcgcc
      181 agcgcagtca actgccgtgc cttcgcctcc gcgatctcga ttccgccgag gcgcggatcg
      241 tggcagacga cggccttcga gtcagcggtg aggcgaacgt caaattcaaa tccgtcgcag
      301 ccgtcggcca gcgcctgatc gaacgaagcc agagtgtttt ccgggattga cttgaatgcg
      361 cgcgcgccgc gatgaccgag gagaagaggg cgtaggctcc gcgggcgggt catcgtgcga
      421 tcaatcgaga tcgaaatcgc ggatcggctt gcctgtgtct ggagtttcct tcgccttctt
      481 cagcgcctcc tgaaacttct tctccaggag gtcggcggcg tgcttctggg cctcgaccga
      541 ctgctgaaag atcgactcgc ggcggctgtc ctgctcgcgc attgctttcg cggccgcttc
      601 catatcgctg aaagtcttgg gcttcacagc cgcggtatgg gcaatcacgg cgcgcgtggc
      661 tgtgtcgatt ttgagcacgg cgctgcaaca ggggcaggtc acttcgaaat tcgcgggact
      721 cttcggcatc tgagcgaatc ataccgcaaa agcagtggtc agtggtcgct ggcagtgaag
      781 tgattagtga aaaaccgggt catgctcagg tattgcttct gctgccagtg agggccacta
      841 ccgtttgagc ttaatgggaa agacgtggaa caggttcagc atctcccagc ccagcaggaa
      901 cattgaaatg aggaccacca tcacggccag caattcgccg aaggtgaggc cgcggcgagc
      961 cggttcgaaa cgccgggcgg ccaggctgag gatgttcagg gtagcaaagc cgaagacgac
     1021 cgcccagagg atgttgtaga cgtcctgccg cgatccgcga tagaggagcg ctacggccac
     1081 gctgggaaac agcgcagctt gaagaccagc gtagatgcgg gcgacgggat acagggcaag
     1141 cgccgcccgg gtagtgcggg cgaaaccgga aagcatcgac atggcggcag gtagcgccat
     1201 cggctggaat gacgaccaga ggccgcgctt cgcggcaggg ccggacctat ggatattaca
     1261 ttttttccgt cgcttggtca cgatggttgt gcctgcgccc cggcaagacg aggcctgatg
     1321 cggttgggaa ctgcacgtca ctcccggcgt tggttaatat ggatcgatgg ccaggctccg
     1381 cttcatcttc ttcgacgtgg gcaacacgct gctgtttccc aaccgtgccc ggatcctcgc
     1441 gccgcttccg caagaccgtc atcccaacct ggagcattgg caggcgctgg agcggcgcac
     1501 caagcaggaa ttcgaccggg gaatgatggg aggcaaggtc gatcacggct tctggtggac
     1561 gtttcatacc tatctgctgg atgagttgaa cctgccggcg gacggggtgc gcgacttact
     1621 ggttcaaaat acgcagcaat ccgctaactg ggaccagatt ctgccgggta cacgggccgt
     1681 tctggaacgg atcgggcagc agtatgcaac ggccgtgatc tcaaacgccg atggcaggat
     1741 cgacgccgtg ctgcggcgct gcgcgattgc cgattgcttt cacagcatca cggattcagg
     1801 catcgtgggg caggagaagc cgcatcccgc aattttcgag gcggcgctgc gcgagatgaa
     1861 ggccgagccg gcggagagtc tctacgtggg tgacgtgtac tcggtcgatt acgttggcgc
     1921 gcgcaatgcc ggcatggagg cggttctgtt cgacgtggcg ggagcgtacc gcgaacgcga
     1981 tttgccgcgg gtggagtcgt tggacgagtt ggggacctgg ctgctaaaag cgtaatgata
     2041 attcagacgt aggagtcatc ccactatgtt tgattcggca cgattcgttc tctttctgac
     2101 agcagcgctg ctgctggcag tcgcacccgg gcctgggatg ctctatgttc tggcgcgctc
     2161 tctcgctggc ggcaagcgcg agggcgtgct gtcggcgctg ggtacgttcc tgggcggaat
     2221 ggtgcatgtg ctggcggcag cgctggggat ttcgatcatc ttggcgaagt cggcggcggc
     2281 gttcgcggca gtgaagtacc tgggcgctgc ctacctgtgc tttctggggg tacggatgat
     2341 cctggatgtg cgaaaggagc gggctggggt ggaagtaatt cccgatcttg gtcgggccgc
     2401 gcggaatcca ctgtggcaag gcgtagcgac cgaagttctg aatcccaaga cagcgctgtt
     2461 cttcctctcg ttcattcctc agtttgtggt ccgtggcgat gggcacgtct tcctgcaatt
     2521 cgtagcgttg gggacgattt ccgtggtgat gaacacaacc gcagatttga tggtgattgc
     2581 gctcgccggg cctctgggag agaagattcg ttcgtcggca accttccgcc ggcgtcagag
     2641 aacggtcacg ggcgcgatca tgattggact gggaacctac ctggcgacga gcgagagcaa
     2701 gtgactcggg cggagcatgt cgcacggaga tgccccaaag ctgggcggca acctcggtgc
     2761 cgcccgcctc tggtgtagat gctagtctcc cgttccgcag gtgcagaacc ccctgccact
     2821 gcccgacagc tcgagaaatg aacattggag ctgcaggctt gagagttcgc ttgggttgag
     2881 cgtcgcatcc tgggaagccg tttcggtcag atggttgtcc tcctgcaggt gagaagccca
     2941 ggcccggacg cccgggatg
//
LOCUS       DASPUL010000158         6803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1672800, whole genome shotgun sequence.
ACCESSION   DASPUL010000158 DASPUL010000000
VERSION     DASPUL010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672800"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VEJ00_03850"
     CDS             <1..392
                     /locus_tag="VEJ00_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXX00325.1"
                     /translation="SYIELVVSSSKSLIDKSRSEIVDLALAEVREFFPAAREATLVKA
                     TVIKEVNATYSPRPGIDAHRPSANTEWPRVFLAGDWTATGWPATMEGAVRSGYLAAEA
                     LARGAGRKDAKFLSPDLPPNGFMRLFA"
     gene            complement(551..946)
                     /locus_tag="VEJ00_03855"
     CDS             complement(551..946)
                     /locus_tag="VEJ00_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00326.1"
                     /translation="MTPYGPVRKRWLALATLAVFAAPILAQSAPPKYDPATETKLKGV
                     VDQLKLVPPTGGKPLVYLLMKEKDSAEVFLCPKSFLDQMGVDFKAGDEIEIVGSKVKQ
                     DSADLILTREITKGGDTLTLRFKDGKPAW"
     gene            complement(1099..1818)
                     /locus_tag="VEJ00_03860"
     CDS             complement(1099..1818)
                     /locus_tag="VEJ00_03860"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HXX00327.1"
                     /translation="MHRMALLVLVALLSVSAGHSSSPAADWAINATAIEACSCPHFCI
                     CYFNAHPAAHHENGKTEHFCKFNNAYKVNKGHYGSVDLTNAKFWLSGDLGGDFSQGQM
                     ESGLVTFDKATTADQRQALGEILGHVFPVKWKNFATAEGDIDTWTFDRDQAHATLNGG
                     KTAEIKLKRFQGMTDEPAVLKNVRYWGTPRNDGFVMMPNEIETYREGPHPYEFKGTNG
                     FMLTFDMTSKDLAPAKGSSMY"
     gene            complement(1912..2292)
                     /locus_tag="VEJ00_03865"
     CDS             complement(1912..2292)
                     /locus_tag="VEJ00_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXX00328.1"
                     /translation="MKALYTWTAILAIVVASVIGDILLARAMKQVGDVHALWRRCSLF
                     AIVGRTLANPNFLLGVIAMAVAFFSLLFALSWGDVSLVAPAAASLTFIGNAFAAKVFL
                     HEKVDRRRWVAAVLVATGVALLAG"
     gene            complement(2490..3650)
                     /locus_tag="VEJ00_03870"
     CDS             complement(2490..3650)
                     /locus_tag="VEJ00_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXX00329.1"
                     /translation="MRRVYLDNNATTPLLPEVFEAMRPYFSEQFGNASSIHHHGQGTR
                     AAVESARESVAALLGSRPSEIVFTSGGTEADNLAIAGLVAPGDHVITSAIEHHAVLLT
                     CKHLEETGCDVTYLPVDGRCLVDPDDVRRALRSNTRLISVMMANNETGVLQPFAEIGK
                     IAAEAGILFHTDAVQAAGKVTVDVAKIGCHALSISAHKLHGPQGVGALYVRKGTQIRP
                     LFYGGRHERSRRAGTENVAGIVGLGKAAQLAKEALDRGDDRQTAAMRDRLQQGILAQV
                     GEAGVNGDTASRVPNTTNIHFDYIEGEAMVIAMDLKGLAASTGAACSSGAIEPSHVLL
                     AMGLQPDQARSSIRFSLGKQTVAEDIDFALALVLETVARLRELSPVHKTRGV"
     gene            complement(3764..3976)
                     /locus_tag="VEJ00_03875"
     CDS             complement(3764..3976)
                     /locus_tag="VEJ00_03875"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXX00330.1"
                     /translation="MMNYVVVYEKSSTGWAAYVPDLPGVVTTGGTKEETQQLIREAIQ
                     FHLEGLREDDLPIPEPSASVEIVTVP"
     gene            complement(3973..4158)
                     /locus_tag="VEJ00_03880"
     CDS             complement(3973..4158)
                     /locus_tag="VEJ00_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXX00331.1"
                     /translation="MKVRDIIRLLEQDGWVLKRTKGSHRQYKHPTKIGRVTLPGHPND
                     DLPPGTLNSVLKQAGLK"
     gene            complement(4180..5322)
                     /locus_tag="VEJ00_03885"
     CDS             complement(4180..5322)
                     /locus_tag="VEJ00_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HXX00332.1"
                     /translation="MSSVTSTHAPKGLEGIVATTSSICYIDGDRGVLAYRGIDIHELA
                     DHSSFEETCYLLWFGKLPTRAELRGLHERLAAERKLDPAIINFLREAPRSALPMDVLR
                     TAVSALSFYDPDNRKNDHEANVAKSIRLTSQIAMIVAAYDRIRKGQPVVESDPSLSHA
                     ANFLLLLNGKRPSSTAERALDIALILHADHELNASTFAARVTAATLSDMHSAITSAIG
                     ALKGPLHGGANEAVFKILETIAANGSDPIDHIKGMLAQKKKVPGFGHRVYHTEDPRAT
                     HLRAMSRDLGRSSGEPKWYEMSEKIEAFVKAEKKLNANVDFYSASTYHVLGIDMDLFT
                     PVFAVSRISGWTAHVIEQLDDNRLIRPRADYVGPEYPARYVPIDQR"
     gene            complement(5466..6308)
                     /locus_tag="VEJ00_03890"
     CDS             complement(5466..6308)
                     /locus_tag="VEJ00_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXX00333.1"
                     /translation="MITDCHIHIQPMEMFKPEALELMKKKRANFEQIAEFCRSPKAFL
                     KYLDASGIDRAMLINYVAPEVIGFTSGVNQFVAEYVKENPRRLLSCGSLHPRHTTNVL
                     ADVEQILRLGLQMIKIHPPHQLLFPNDYLSGAKELEIIYRAAEANGVPVMFHTGTSIF
                     PGARNKYGDPIYVDDVAVDFPKLKILLAHGGRPLWMHTAFFLIRRHPNVYLDISGIPP
                     KTLLKYFPRLEEIDHKTLFGTDWPGPGVPDIKKNLEEFRALPLSAAVQDQILSKTALS
                     IWPA"
     gene            complement(6321..>6803)
                     /locus_tag="VEJ00_03895"
     CDS             complement(6321..>6803)
                     /locus_tag="VEJ00_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00334.1"
                     /translation="RDPVELEWLIAQSVKLKAEVVSADEREEGLRRVLNLGHTIGHAL
                     EAETGYRRLLHGEAVAWGMIAATNIALNVGRTDSVTAGRIADAVLSLGRLPEVNVSPR
                     KIMARLQSDKKTQNGVVHFILPREIGKVEVASDVPESAVMQSLDELRRLSRGGWVWRK
                     "
BASE COUNT         1339 a         2006 c         2081 g         1377 t
ORIGIN      
        1 gcagctacat cgaactggtt gtaagttcgt cgaagtctct gatcgacaag tctcgctccg
       61 aaatcgtcga cctcgctctc gccgaagtgc gcgagttctt ccctgccgcg cgtgaggcga
      121 ctttggtgaa ggccaccgtg atcaaggaag tgaacgcaac ctactcgccg cgcccgggca
      181 tcgacgcgca ccgtccatcg gcaaacacag aatggccgcg cgtttttctc gccggagact
      241 ggaccgcgac cggctggcca gcaaccatgg aaggcgcggt ccgcagcgga tacctggcag
      301 ccgaagcgct ggcgcgcggc gccgggcgca aggatgccaa gttcttgtct cctgacctac
      361 ccccgaacgg attcatgcgg ctcttcgcct gaatccggac ttacaagatt ttgaccttca
      421 ccgcgcgctc ctgaaaatgc gttttctttg cgctcaccgc ggccattcct tcgcgactct
      481 gcggtgaaaa gcctttgagc gcagagatcg caaagaaaag ccgcaaagga cgcgaagaaa
      541 agcgaagatc ctaccaggcg ggcttgccgt ctttgaagcg cagggtgagc gtgtcgccgc
      601 cttttgtaat ctcgcgtgtc agaatcaagt ccgcgctatc ctgcttcacc tttgaaccga
      661 cgatctcaat ctcgtcgccg gctttgaaat caactcccat ctggtcgaga aagctcttcg
      721 ggcaaagaaa gacctcggcg gaatccttct ctttcatcag gagataaacg agcggcttgc
      781 ccccggtggg aggaacgagt ttcagttggt cgaccacacc cttgagcttg gtttcggtgg
      841 cggggtcata tttgggtggc gcggactgcg caagaatggg ggcggcaaac acggcgagcg
      901 tggcgagtgc gagccaacgt ttccgcactg ggccgtacgg agtcatcgca aggccctcca
      961 gaggcgaaat gttaggcccg gccgccatca aaaagcaacg gatttctgcg cgtaccccgg
     1021 tattcgccaa gctcgtcccc gtccaagaaa aaatgcgccg gaggaatccg gcgcatccgg
     1081 tctcttgaaa acattcattc agtacatcga ggagcccttg gccggggcga ggtctttcga
     1141 ggtcatgtca aaggtcagca tgaacccgtt cgtccctttg aattcgtatg ggtgcgggcc
     1201 ttcccggtag gtttcgatct cgttgggcat catgacgaag ccgtcgttgc gcggcgtgcc
     1261 ccagtacctg acgttcttca gcacggcggg ctcgtcggtc atgccctgaa atcgcttcag
     1321 tttgatctca gccgtcttgc cgccgttgag cgtggcatgg gcctggtccc tatcgaacgt
     1381 ccaggtgtcg atgtcgcctt cggctgtggc aaagttcttc cacttcacgg ggaagacatg
     1441 acccaggatt tcacccagcg cctgccgctg gtcggcggtc gtagccttgt cgaaggtcac
     1501 cagcccagac tccatctggc cctgcgaaaa gtcgccgccc agatcgccgc tgagccagaa
     1561 tttggcgttg gtcaggtcga cgctgccgta gtgtcccttg ttgaccttgt acgcgttgtt
     1621 gaacttgcaa aaatgctcag tcttgccgtt ctcgtgatga gcggcaggat gcgcgttgaa
     1681 ataacaaatg cagaagtgtg gacagctgca ggcttcgatc gcggtcgcgt tgatcgccca
     1741 gtctgcggcc ggtgaagagg aatgccctgc gctgacagac agcagggcaa caaggactag
     1801 caatgccatt ctgtgcatgg gagccgcacc cccagatgcg cgacagtatg cgcctgcgcg
     1861 ggtggcattg caacagttgt ggtctgtcag gtgtcaggag tgtacctgag cctagcctgc
     1921 gagcagggct acgccagttg cgaccagcac ggcggcgacc cagcggcgcc ggtcgacttt
     1981 ctcgtgcagg aagaccttgg cggcaaacgc gttgccgatg aaggtaagcg aagcagcggc
     2041 gggtgcgacc aggctaacgt ctccccagga gagggcaaac agcagactga agaaagcgac
     2101 cgccatcgcg atcacgccaa ggaggaagtt gggattggca agggtacgcc caacgatggc
     2161 gaagaggctg cagcgccgcc agagcgcgtg cacgtcgccg acttgcttca tcgcgcgcgc
     2221 aagcaggatg tcgccaataa ccgaagcgac cacgatagcg agtatcgcgg tccaggtgta
     2281 aagggctttc atgagcaggt aatctatagg ccgcttcgtg cggcctcaca ctttcaaatc
     2341 caatggtaat tcacttgtca tgctgagcga agaacgagat ctcgcagcga actcgttccg
     2401 aagcatccct accccaaaat gccgtgccga aagtagggat tcctcgctgc actcggaatg
     2461 acagcaccgg ctttggattg cagcatctcc taaacccctc gagtcttatg gacaggagac
     2521 agttccctca gtctggccac ggtttcgagc acgagtgcca aagcgaaatc gatgtcctcc
     2581 gcaaccgttt gcttgcccag gctaaaacga atgctggacc gcgcctgatc cggctgcagg
     2641 cccatcgcca gcagcacatg cgacggctcg atcgctcccg aagagcaagc agcgccagtg
     2701 gaggccgcca ggcctttcaa gtccatcgcg atcaccatcg cttcaccctc gatgtagtcg
     2761 aagtgaatat tagtagtgtt gggcacgcgc gaggcagtgt cgccgttcac gccggcctca
     2821 ccgacctgcg cgaggattcc ctgctgcagg cggtcgcgca tagcagccgt ctggcgatca
     2881 tcgccgcgat ccagagcctc cttcgccaac tgcgctgcct tccccaatcc gacgatcccg
     2941 gcaacattct cggtgccggc acgccgcgag cgctcatggc gtccgccgta gaacagcggc
     3001 cggatctgag ttcccttcct cacgtagagc gcgcccacac cttgcggccc gtgaagcttg
     3061 tgggctgaaa tcgaaagcgc gtggcagccg attttcgcca cgtcaaccgt gacctttccc
     3121 gcggcctgca ccgcgtcggt gtggaagagg atgccggctt ccgccgcgat cttgccgatc
     3181 tccgcgaacg gctgcaggac tccggtttcg ttgttcgcca tcatgacgga aatcaggcgc
     3241 gtgttcgagc gcagggcgcg gcggacgtcg tcggggtcga ccaggcatcg gccgtccacc
     3301 gggagatacg tcacgtcgca gccggtttcc tccaggtgct tgcaagttag caggactgca
     3361 tgatgctcga tcgccgaagt gatgacgtgg tcgccaggag caacgaggcc ggcgatcgcg
     3421 agattgtcgg cttcggtgcc accactggtg aaaacgatct cggacggtcg gctgccgagc
     3481 aaagcggcga ccgactcgcg tgcgctttcg accgcagctc gcgtcccctg cccgtgatga
     3541 tggatcgagg aggcgttgcc gaattgctca gaaaaatacg gacgcatcgc ttcgaacacc
     3601 tcgggcagaa gtggcgtagt ggcgttgttg tcgaggtaaa cgcggcgcat gcagaaccct
     3661 gagggtattc tagccgaggc gcgtgctggg gcgtcaaagc tgggaaggtg atctacaggc
     3721 taactattgc ggtcatgatc gtggagaatc gctccgagcg ggttcaagga acggtcacaa
     3781 tctccacaga ggctgagggc tcgggtatcg ggaggtcgtc ttcgcgcaga ccttcgagat
     3841 gaaactgaat ggcctcgcga atcagctgct gagtttcttc ctttgttccc cccgtggtca
     3901 ccacgcccgg caagtccggc acataggccg cccagccggt agaagatttc tcatagacga
     3961 cgacgtagtt catcatttca agcctgcctg tttcagcacg ctgttcaagg ttcccggcgg
     4021 aaggtcgtca ttgggatgac ccggcagagt cacacgccca atcttcgtcg gatgtttgta
     4081 ttgtcgatga ctgcccttgg ttcgctttag cacccagcca tcctgctcca acaatcgaat
     4141 gatatcgcgg accttcatga tttcaaaatc cgcaatccgc taccgctgat cgatgggcac
     4201 gtaccgggcg ggatactccg gcccgacata gtcggcgcgc ggacggatca ggcggttgtc
     4261 gtcgagctgc tcgatcacgt gtgcggtcca gccgctgatg cgggagaccg caaagacggg
     4321 agtgaagagg tccatgtcaa tgcccagcac gtggtaagtc gaggcggagt agaaatccac
     4381 gttggcgttc agcttctttt ctgccttaac gaaggcttca atcttttcgg acatctcgta
     4441 ccacttgggc tcgccgctgg agcggcccag atcgcgcgac atggcgcgca ggtgcgtggc
     4501 ccgcggatcc tctgtgtgat acacgcgatg gccgaagccg gggaccttct tcttttgcgc
     4561 gagcattccc ttgatgtggt cgatcggatc gctgccgttg gcggcgatgg tctcgagaat
     4621 cttgaagacg gcttcgttgg cgccgccgtg caacggccct ttcaaggctc caatcgccga
     4681 agtgatggca gagtgcatat ccgacaaggt ggctgccgtc acgcgcgcgg caaaggtcga
     4741 ggcgttgagt tcgtggtcgg catgaagaat cagggcgata tccagggcgc gctccgccgt
     4801 ggaggaagga cgcttgccgt tcagcaggag cagaaagttg gccgcatgcg aaagcgacgg
     4861 gtccgactcg accactggct gacctttgcg gatgcggtca taggccgcga cgatcatggc
     4921 gatttgcgag gtcagtcgga tggacttcgc cacgtttgcc tcgtgatcgt tcttccggtt
     4981 gtcgggatcg taaaacgaaa gcgcggagac ggccgtgcgc agcacgtcca tgggcaaggc
     5041 cgaacggggc gcttcgcgca ggaaattgat gatcgccggg tccagcttgc gttccgccgc
     5101 caggcgctca tgcagcccac gcagttcggc gcgcgtcggc agctttccga accagagcag
     5161 gtagcaggtt tcttcaaagc tggagtgatc agctagttcg tgaatgtcga tgccgcgata
     5221 ggcgagaacg ccgcggtcac cgtcgatgta gcaaatactg gaagtcgtgg caacgatgcc
     5281 ctcaagtcct ttcggggcat gggtgctggt tacgctggac attcgcctct catctaagct
     5341 cgctgaatct ttcgttgggg cccacagcag cgacgcggta gatacaacct tgtttgttta
     5401 tacctgaaac gcgcggaact ttccaagagc gggagggcat ggcccgccgg acagtcgaag
     5461 cggattcagg ccggccagat gctgagcgcg gtcttgctga ggatctggtc ctgaaccgcg
     5521 gcactcagcg gaagagcgcg aaattcctcc agatttttct tgatatccgg cacgcccggt
     5581 ccgggccagt ccgtgccgaa cagcgtcttg tggtcgattt cttccaggcg cgggaagtac
     5641 ttcagcagcg tcttgggcgg aatcccgctg atgtcgaggt agacgttggg atgccgccgg
     5701 atcagaaaaa acgcagtgtg catccagagc ggacgcccgc catgggccag caagatcttg
     5761 agtttaggga aatcgacggc cacgtcgtcg acataaatcg gatcgccgta cttgttgcgc
     5821 gcgccgggaa agattgaagt ccccgtgtgg aacattaccg gcacgccgtt ggcttcggcg
     5881 gcgcgataga taatctccaa ctccttcgct ccgctcaggt aatcgttggg aaacagcaac
     5941 tggtgcggcg gatgaatctt gatcatctgc agcccgagcc gcaggatctg ttcgacatcg
     6001 gcgagcacat tcgtggtgtg ccgcggatgc aggctgccgc aggacagcag gcgccgcgga
     6061 ttttctttca cgtactccgc gacgaactgg ttcacgccgc tggtaaaacc gatcacttcg
     6121 ggggcgacgt agttgatcag catggcgcgg tcgatgccgg acgcatccag atatttcagg
     6181 aaggccttcg gcgagcggca gaattcggcg atctgctcga agttggcccg cttcttcttc
     6241 atcagctcga gcgcctcggg cttaaacatt tccatcggct ggatgtggat atggcaatcg
     6301 gtaatcatca gtggataggc ttactttctc cagacccaac cgccgcgcga caggcggcgc
     6361 aactcgtcca gggattgcat gaccgcgctc tcgggaacgt cgctggcaac ttccactttg
     6421 ccgatttcgc gcggcaggat gaaatgtaca actccgtttt gcgttttctt gtcgctctgc
     6481 agccgggcca taatcttgcg cgggctgacg ttcacttccg gcaatctccc caggctgagc
     6541 acggcgtcgg cgatgcggcc ggccgtaacg ctgtcggtgc ggccgacatt gagcgcaatg
     6601 ttcgtggccg cgatcatgcc ccaggccaca gcttcgccat ggagcagccg ccggtatccc
     6661 gtctccgcct caagtgcgtg gccgatagtg tggccgagat tgagtacccg ccgcaggcct
     6721 tcctcgcgct cgtcggcaga aacgacctcg gctttcagct tcacggattg agcgatcagc
     6781 cactccagct ccacgggatc gcg
//
LOCUS       DASPUL010000159         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_984254, whole genome shotgun sequence.
ACCESSION   DASPUL010000159 DASPUL010000000
VERSION     DASPUL010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984254"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VEJ00_03900"
     CDS             complement(<1..831)
                     /locus_tag="VEJ00_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXX00335.1"
                     /translation="MLTRRHFLSAATTVATGLAFRRDLLAQLEKIPPLPERSLLDKDE
                     DAFWAAMRRQFLIPEDEVYLNNGTVGSSPAPVLRAIFDGYTTTERMDQEDPEDYPIWG
                     YAAWNEFRDPLAAFVGCNHDEIALLRNATEANSYIANGVDMKAGDEVLMTDQEHPGGE
                     HPWNLKAKRYGIVVRKVTLPRPVKDAAQVLNLFNDAIAPRTRVLFFSHITTFSGVVLP
                     AKELCALARSKGIMSAVDGAHVPGMMRLNVHDLGCDMYSSSPHKWLFAPKGSGFLYVR
                     D"
     gene            1050..>2210
                     /locus_tag="VEJ00_03905"
     CDS             1050..>2210
                     /locus_tag="VEJ00_03905"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXX00336.1"
                     /translation="MKLRWLAIAALAAFSVVTWAQTQTPNQNPGSTQTQTQQQPQSQP
                     PATDPDQGQPKAGDVQNAPPQKPATLPTQDDSQIKHDGGKTDVDAVGNRNVGCGRGVG
                     NWYSVEGQVARGRVYAQQIESQIKLVNDPVVTEYVNRIGQNLVRNSDAQVPFTIKVID
                     SDVVNAMALPGGFFYVNSGLILAADEEAEMAGVMAHEIAHVAACHYGREMTRANLLQI
                     ASLPAIFMGGALGYGIYEGMGLGIPLTFLHFSRGFEAEADYLGIEYMYRAGYDPSAFV
                     SFFEKIQAMEKKKPGTLSKAFDTHPQTPDRIEKSQEEIRKILPAKAQYVVTTSEFDEV
                     KARLAAIENKHKVLDQKDANKPSLRRTANSGDNTGDQKKDDDRPTLKRRDDSN"
BASE COUNT          467 a          637 c          723 g          383 t
ORIGIN      
        1 atcccgcacg taaagaaagc cggagccctt gggtgcaaac agccacttgt gcgggctgga
       61 ggaatacatg tcgcaaccca ggtcgtgcac attcagtcgc atcatgccgg ggacgtgagc
      121 gccgtccacc gctgacataa tccccttgga acgcgccaag gcgcacagtt cctttgccgg
      181 caacacgact cctgaaaaag tcgtgatatg gctgaaaaac aggacgcgcg tgcgcggcgc
      241 gatggcgtcg ttgaagagat tcagcacctg ggctgcgtcc ttgacggggc ggggaagcgt
      301 aactttcctg acaacgatgc catagcgctt ggccttcagg ttccacgggt gctcgccgcc
      361 gggatgttcc tggtcggtca tgagcacctc gtctccggcc ttcatgtcga cgccgttggc
      421 gatgtaactg ttggcttcgg tggcgttgcg cagcagggcg atttcgtcgt ggttgcagcc
      481 gacaaaggcc gccagaggat cgcggaactc gttccaggcg gcgtatcccc agatgggata
      541 gtcttccgga tcctcctggt ccatcctctc ggtggtcgta tagccgtcga aaatcgctcg
      601 cagcacgggc gcggggctgg aaccgaccgt gccgttgttc aggtagacct cgtcttccgg
      661 aatcaggaac tggcggcgca tggcagccca gaaggcgtct tcgtccttgt cgaggaggga
      721 ccgctcgggc aaagggggga tcttctccaa ctgagcgagc aaatctcgac gaaaggcgag
      781 tccggtggca acggtcgtcg ccgccgaaag gaaatgacgt cgggtgagca tggaagcctc
      841 cttggcaggg gcctagagga cgcgccatag tacgaccacg agccggaggc gggcaagggg
      901 ttgtgtggcc atttcgccca taggtttgta gaacttccgg tggggggcgc gggtaacccg
      961 gaggcggtat tgggcgtcta ataacaaggg ccaggttcag cgttgggtgt gcacaagtgg
     1021 ggcgtataat cggtctcaag gagtacccta tgaaactcag atggctggca atcgcggcgt
     1081 tggcagcgtt cagcgtggtg acctgggcgc agacacagac tccgaatcag aatcccggtt
     1141 cgacccaaac tcagacgcag cagcagcccc agtcgcagcc gcccgcgacg gacccggatc
     1201 agggccagcc caaggcaggc gatgtgcaga atgcgcctcc gcagaagccg gcgacgctgc
     1261 cgactcagga tgacagccag atcaagcatg acgggggcaa gaccgacgtc gatgccgtcg
     1321 ggaaccgcaa cgtgggctgc ggccgcggcg tgggcaactg gtactcggtc gaaggacagg
     1381 tagcgcgggg ccgggtctat gcgcagcaga tcgagtcgca gatcaagctg gtcaacgatc
     1441 ccgtggtcac cgagtatgtg aaccggatcg gccagaacct ggtgcgcaac tctgacgcgc
     1501 aagttccgtt cacgatcaag gtcatcgact ccgacgtggt caacgccatg gcgctgcccg
     1561 gcgggttctt ctacgtgaac tccggcctga ttttggccgc tgacgaggaa gccgaaatgg
     1621 ctggtgtgat ggcgcacgaa atcgcgcacg tggccgcctg ccactacggc cgcgaaatga
     1681 cgcgcgccaa cctgctgcag atcgcttcgc tgcccgcgat cttcatgggc ggcgcgctcg
     1741 gctacggaat ttatgaaggc atgggcctcg gcatcccgct gacgttcctg cacttctcgc
     1801 gcggatttga agctgaagct gactacctcg ggatcgaata catgtaccgt gcgggctacg
     1861 atccgtcggc cttcgtctcg ttcttcgaaa agattcaggc gatggagaaa aagaaaccgg
     1921 ggacgctgtc gaaagcgttc gatacgcacc cgcagactcc ggaccggatc gagaagtcgc
     1981 aggaagagat tcgcaagatt cttcccgcta aagcgcagta cgtcgtgacc acctcggagt
     2041 ttgacgaggt gaaggcgcgg ctggcggcaa tcgagaacaa gcacaaggtg ctggatcaga
     2101 aagacgcgaa caaacccagc ctgcgccgga cggcgaacag cggcgataac accggcgacc
     2161 agaagaagga cgacgatcgt ccgaccctga agcgccgcga cgattcgaac
//
LOCUS       DASPUL010000160         4422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1532073, whole genome shotgun sequence.
ACCESSION   DASPUL010000160 DASPUL010000000
VERSION     DASPUL010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532073"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VEJ00_03910"
     CDS             <1..190
                     /locus_tag="VEJ00_03910"
                     /inference="COORDINATES: protein motif:HMM:NF042701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM hydroxide adenosyltransferase"
                     /protein_id="HXX00337.1"
                     /translation="EITDIHAAYAEGEPGEIFGILGSMGYLEIAANRGAAAQITGAGK
                     GSEVSIVLGEGAAAGKGA"
     gene            complement(261..494)
                     /locus_tag="VEJ00_03915"
     CDS             complement(261..494)
                     /locus_tag="VEJ00_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00338.1"
                     /translation="MRHRLRFLWNATKGHHLAPWRSPYLLWRIETYTGVKMTQIGFLE
                     FWEFMWRERRELWRFLRWTGEMERYVHPKPKSS"
     gene            769..1134
                     /locus_tag="VEJ00_03920"
     CDS             769..1134
                     /locus_tag="VEJ00_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX00339.1"
                     /translation="MPDDPIPPSSARQKAEDHYYAALDLMADGNLEKAVAAYQESVAA
                     DPMFTEAMHGLARALQDLQRYDEAIAVAQRIAEIDSDDVLAHTSLSVLYMKRGMIAEA
                     EAEGAKARVLGWKQQLKKG"
     gene            complement(1131..1751)
                     /locus_tag="VEJ00_03925"
     CDS             complement(1131..1751)
                     /locus_tag="VEJ00_03925"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXX00340.1"
                     /translation="MKSPLRHLPFRRRPAAALLVTVLISFSSACHRSDADVTEILRAA
                     GNGDLSTTRLLLNRNPSLVFGKNSEGITALHYAASYGYKEIAELLLSNKANVDARDGV
                     GRTPLYDAAAAGRIEEVRILLAANAAVNMGTLRGYTPVSGAASHGREDIVRLLIDNGA
                     NVSAADLDGGTPLYWARIRGYDRIQDLLHQHGGLELPRAKPEWPYP"
     gene            2035..2490
                     /locus_tag="VEJ00_03930"
     CDS             2035..2490
                     /locus_tag="VEJ00_03930"
                     /inference="COORDINATES: protein motif:HMM:NF039408.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HXX00341.1"
                     /translation="MEDIRVRLSVLWLFATLNYIYADVFFCVDVLGSGKGGPGVLHFS
                     QGAWLGIAILMEIPMAMILLSRILKPTANRWANIVAGVIETAAVLLTSFILPAFHLTG
                     TSSYYMFFGAVEVVCTSLIVWYAWRWPREAARRIEPSGQPLVPAAIELP"
     gene            complement(2565..3959)
                     /locus_tag="VEJ00_03935"
     CDS             complement(2565..3959)
                     /locus_tag="VEJ00_03935"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXX00342.1"
                     /translation="MANILVCDDERSICEMLDISLRREGHRVETVQSGQTAKNKIDGA
                     LYDVIITDIKMPNVDGIEVLRHAHRVSPDSAVILITAVDDYEAAVEAVKAGGASDYIR
                     KSPGLVDEIKLALNRSLEKLVLSRQNFALRRDAATRNSLDNIIGSSAAIEKLKQTVRT
                     VASTASTVLIHGESGTGKELVARAVHVCSPRATEAFVSVNCGAFPETLLESELFGYVK
                     GAFTGANQNKRGLFEVADRGTIFLDEISEMTLAMQVKLLRVLQERTVRPVGSAAEISI
                     DVRVIAATNRDLDKAVAENLFREDLYYRLNVIPILVPPLRERPEDIPLLVNHFLKKYA
                     TAAGRSILRVHAGSLSSLCDYDWPGNVRQLENTVERAVALETSAELHVELPVERPKAR
                     AAAAAAGGGTLPDISGEITLPDGIGMENYVAGIERSLLQTALHQSGGVQTKAADVLGI
                     SYRSFRHLMKKYGL"
     gene            complement(3952..>4422)
                     /locus_tag="VEJ00_03940"
     CDS             complement(3952..>4422)
                     /locus_tag="VEJ00_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXX00343.1"
                     /translation="FTVRQAPTIADGDKIKQVFWNICENAVRAMTAGGTKAGGTLTVA
                     VEAFGDDWQIGFTDTGTGMTPQQIEKIFEPFQSNFEGGTGLGLALVYQIVQAHEGKVW
                     ARSRPGQGTTFVLRLHRLDAGHDAATEERALPAEAPAAIPAPPVAAAAAGRSHG"
BASE COUNT          826 a         1283 c         1413 g          900 t
ORIGIN      
        1 ggaaatcacg gatatccacg ctgcctacgc cgagggcgaa ccgggagaaa tctttggcat
       61 tctgggcagc atgggttatc tggagatcgc tgccaaccgc ggggcggccg cgcagattac
      121 cggcgccggc aagggcagcg aggtcagcat cgttctgggc gaaggcgctg ccgccggcaa
      181 gggcgcgtag gagcagtgag cagtggtctg ctgatcttgg gtgttgctgg ccactgacca
      241 ctagccactg accactgcct ctaggaactc ttcggcttgg ggtggacgta gcgctccatc
      301 tcgcccgtcc agcgcagaaa tcgccacaac tcccgccgct cgcgccacat gaattcccag
      361 aactccagaa atccgatctg ggtcattttg acgccggtgt aggtttcgat ccgccacagc
      421 agatagggac tccgccacgg agccaggtgg tggcccttgg tggcgttcca gagaaagcgc
      481 agccggtggc gcatgcaagc atgataacca cgaaggaccc ttgccgacta ggcgctgcat
      541 cggctcttgg ggtgtcatcc cgagcgaagc cgttcttgag gcgaagcgag ggatctcatc
      601 gcaggtcact ctcaggggag atccctcggc ccgctagtga aagcgcgggc cttcgggatg
      661 acgccctcgt ggagagtcgt cattcaaacc gagtgactac cagcaagcca cgaccggctg
      721 tcgctactcg ccgtgctaga atccaggctc cgccagggat ctccccgcat gcctgacgat
      781 ccgattcctc ccagcagcgc ccgccagaag gctgaagacc actactacgc tgcgcttgat
      841 ctgatggcgg acgggaatct ggaaaaggcc gtcgcggcct atcaggagtc agttgctgcc
      901 gatccaatgt tcacggaagc catgcacggc cttgcgcgag ctttgcagga tttgcagcgc
      961 tacgacgagg cgattgccgt ggcgcagcgg attgccgaga tcgattccga tgacgtcttg
     1021 gcgcatacca gcctgtcagt gctgtacatg aagagaggga tgatcgccga ggccgaagcc
     1081 gaaggcgcca aggctcgggt gctggggtgg aagcagcagt tgaagaaggg ctagggatag
     1141 ggccactccg gcttcgcacg cggcagttcc aatccaccgt gctggtgcaa taagtcctga
     1201 atgcggtcgt atccccttat tcgcgcccaa tacaagggcg tgccgccatc gaggtctgca
     1261 gcactcacgt tggccccatt gtcgatcagc agacggacga tatcttcacg accgtgagag
     1321 gcagccccgc tcaccggggt ataaccgcga agagtcccca tgttcacggc ggcattggcg
     1381 gctagaagta ttctcacttc ctcgattcga ccggctgctg cagcgtcata cagcggcgtg
     1441 cgcccgactc cgtcccgggc atcgacgttt gccttgttgg acagtagcag ctctgcaatt
     1501 tccttgtagc cgtatgacgc cgcgtagtga agagcggtga tgccttcact gttcttacca
     1561 aacactaggc tcggattgcg gttgagcaag agccgagtcg tcgacaaatc accgttgcca
     1621 gctgctcgga gaatttcggt aacgtccgcg tcagatcggt ggcatgccga ggagaagctg
     1681 atcaagactg tgacaagcag cgctgcggcg ggacggcggc gaaacggaag gtgacggagt
     1741 ggcgacttca tagcctcgcc tttcggaacc gttaagtata taggggcaca ccgagatcgt
     1801 ggatgctatc tctcagattc gttttgatgc gaccgatagc tacaacccat acttcttcat
     1861 caagtgccgg aaggagtggt aggaaatgcc gaggtcggta gttgagtctt tttccagagc
     1921 tcatccgtag tagaatttga ttccaacttt gggctccgca cgttgccaaa atcgccttag
     1981 ttcagtgcgg taggccgaac atcacggccc gcttgggcag cacaggaggc agccatggaa
     2041 gacattcgag tgagactttc ggtgttatgg ctgtttgcga cgctcaacta tatctatgca
     2101 gatgtgttct tctgcgttga cgtcttgggg tcgggtaaag gaggtccagg tgttcttcat
     2161 ttttcccagg gagcgtggtt aggaatcgca atactcatgg agatccccat ggcgatgatt
     2221 ctgctttcgc ggatcctgaa gcccacagca aatcgctggg cgaacatcgt cgcgggcgtg
     2281 atagaaactg ccgctgttct cttgaccagt ttcatccttc cagcctttca tctcacggga
     2341 acaagttctt actatatgtt cttcggagcg gtggaagtgg tttgtacttc gctgatcgtt
     2401 tggtacgcat ggagatggcc gagagaagcc gcccgccgaa ttgagccttc cggccagcct
     2461 cttgtccctg cagcaattga gctcccctaa taggggtaac tgtgggctac tcgggctttc
     2521 ggttctcgca ggccaaactg aaagctgaga ctgatagccg atagctacaa cccgtacttc
     2581 ttcatcaaat gccggaacga gcggtaggag atgccgagaa cgtcggcggc tttggtctgg
     2641 acgccgccgc tttggtgcag ggcagtctgc agcagcgagc gctcgatgcc ggccacgtag
     2701 ttttccatgc cgatgccgtc gggaagcgtg atttcgccag agatgtcggg cagcgtgccg
     2761 cctcccgcgg cggcagcggc ggctcgggcc ttcggtcgct ccaccggcag ttcgacgtgc
     2821 agctcggcgc tggtttcgag cgcgacggca cgctcgaccg tgttttccag ctggcggacg
     2881 ttgcccggcc aatcgtagtc gcagagagag gaaagcgatc cggcgtgcac gcggagaatg
     2941 ctgcggccgg cggcggtggc gtatttcttc aggaagtggt tgaccaacaa gggaatgtct
     3001 tccgggcgct cgcgcagcgg aggcaccaga atcgggatca cgttcagccg gtaatagaga
     3061 tcttcgcgga aaaggttttc ggcgacggcc ttgtcgagat cgcgattggt agcggcgatg
     3121 acgcgcacgt cgatcgagat ttcggcggcg ctgcccacgg gccgcacggt gcgctcctgc
     3181 agcacgcgca gcagcttgac ctgcatggcc agcgtcattt cgctgatctc gtcgaggaag
     3241 atcgtgcccc ggtcggcgac ttcgaaaagg ccgcgcttgt tctggttggc accggtgaac
     3301 gctcccttga cgtagccgaa cagctcgctt tcgagcaggg tttcagggaa ggctccgcag
     3361 ttgacggaaa cgaacgcttc ggtggcgcgg ggcgaacaca cgtgcacggc gcgggcgacg
     3421 agttccttgc cggtgccgct ttcgccgtga atgagcacgg tactggcggt cgaggcaacg
     3481 gtacgcacgg tctgcttcag tttctcgatg gcggcgctgg acccaatgat gttgtcgagc
     3541 gaattgcgcg tcgcggcgtc gcgccgcagg gcgaagttct gccggctcag gaccagcttt
     3601 tccagggagc ggttcagggc cagcttgatt tcgtcgacca gtcccggact cttgcggatg
     3661 tagtcggagg cgcccccggc cttgacggct tcgaccgcag cctcgtagtc gtcgacggcc
     3721 gtaatcagga tgacggcgga atccggcgaa acccggtgcg cgtgccgcag gacttcgatg
     3781 ccgtcgacat tcggcatctt gatgtcggtg atgatgacgt cgtagagcgc gccgtcgatc
     3841 ttgttcttgg cggtctgccc cgattgcact gtctccacac gatgcccttc gcggcgcagg
     3901 gagatatcga gcatttcgca gatggagcgc tcgtcgtcgc agacgagaat attagccatg
     3961 cgacctcccg gctgccgcgg cggccacggg cggggccgga atcgcggcgg gagcttcggc
     4021 gggcagggcg cgttcctcgg tggctgcgtc gtggccggcg tcgagccggt gcagcctcag
     4081 gacgaacgta gtcccctgcc cgggtcgaga ccgggcccat acctttccct cgtgcgcctg
     4141 cacgatctgg tagaccagcg ccagccccag gccggtgccg ccctcaaagt ttgactggaa
     4201 gggctcgaag attttctcga tctgctgcgg agtcatgccg gtcccggtgt cggtaaagcc
     4261 gatctgccag tcgtcaccga aagcctcgac cgcaacggtc agcgtgccgc cagccttggt
     4321 tcctccggcg gtcatggcgc ggaccgcgtt ctcgcagata ttccagaaca cctgcttgat
     4381 cttgtcgccg tcggcgatgg tcggcgcctg gcggacggta aa
//
LOCUS       DASPUL010000161         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_984379, whole genome shotgun sequence.
ACCESSION   DASPUL010000161 DASPUL010000000
VERSION     DASPUL010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984379"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VEJ00_03945"
     CDS             <1..397
                     /locus_tag="VEJ00_03945"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXX00344.1"
                     /translation="VFPPGHRDSGAGLSSQGDRDPPHVNRISVGSTEIAAHLVHRVQP
                     EYPEIARLEYLQGKVKLHALIATDGSVKYLQVLAGYCSLAEAAQRAVRQWQYGPTLLK
                     NEPVEVDTTIQVVFKLRRQWERHSSSPDY"
     gene            1023..2309
                     /gene="serS"
                     /locus_tag="VEJ00_03950"
     CDS             1023..2309
                     /gene="serS"
                     /locus_tag="VEJ00_03950"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXX00345.1"
                     /translation="MHDLSYFRDHLDIFGDMAQRRGLGLDLGAFRVLDQERRQLITRT
                     EQLKAQRNKASEEIGRLKKEKQDAGPLLAQMKQVSDQIKQADDRISQLDQQLRDFMLT
                     VPNLLHSSVPPGKTSAENVEVRRWGSPPKFDFVPRPHWEIGENAGILDLARAVKIAGA
                     RFALYRGAGARLERALANFFLDVHTKEHSYTEVLPPFLVNTASLVADGKLPKFASDMF
                     HIEGSDFWLTTTAETELTNLFRDETLDADALPVRVCAWTSCFRSEAGAAGKDTRGIKR
                     QHQFQKVELFKFTRPEQSYEELERLVGNAEAILQRLGIHYRVMQLCAGDTGFASSKTY
                     DIEAWLPSSSEFMEISSCSNCEAFQARRAGIRMKSKGTKSEFVHTLNGSGLPVGRTWI
                     AIVENCQRADGSVVIPEVLRPYMGMDRIPALAGSEA"
     gene            2533..2829
                     /locus_tag="VEJ00_03955"
     CDS             2533..2829
                     /locus_tag="VEJ00_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00346.1"
                     /translation="MTFFARLARFLFWLLIVSWGVALLRRIAAWALQRSAPHDVDEPA
                     QASVARRLHRDPVCGTHVSDQISVPLLEAGETLHFCSEECRTKYANLHRPAVNE"
     gene            2869..>3512
                     /locus_tag="VEJ00_03960"
     CDS             2869..>3512
                     /locus_tag="VEJ00_03960"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HXX00347.1"
                     /translation="MATVAKPTAPPMVPRIAPREDLNPFRIAQIQFDMAAEFLKLDPS
                     LRQILRTPKRVLEVSIPTKMDNGQVKVLTGYRVQHNVARGPGKGGIRYHPGVTVDEVK
                     ALAAWMTWKTATVNVPLGGAKGGVICDPKRMSKGELERMTRRYASEILPIIGPDQDIP
                     APDVYTDSQTMAWIMDTYSMTIGRNAHAVVTGKPLSLGGSAGRDEATARGLIYVV"
BASE COUNT          706 a         1076 c         1037 g          693 t
ORIGIN      
        1 cgttttcccg ccggggcacc gggattcggg agcgggacta tcctcgcagg gcgatcgcga
       61 tccgccgcat gtgaaccgca tcagcgtggg aagcaccgag atagccgccc acctcgttca
      121 tcgcgtccag ccggaatacc cggagatcgc ccggctggaa tacctccagg gcaaagtaaa
      181 actccacgcc ctgattgcga ccgacggttc ggtgaagtac ctccaggtgc tggcgggata
      241 ctgctcactg gcagaagccg ctcaacgggc ggtgcgccaa tggcaatatg ggcccactct
      301 gctgaaaaac gaaccggtcg aagtagacac aacgattcag gtcgtcttca agctgcgacg
      361 ccagtgggaa cggcacagct catcgcccga ctactaagcc tctagggcgt ggcgccgtta
      421 ttcctccaaa agaatggagt ggggacgcgg cctctctgtg agattctgta gagggaacta
      481 tccgattttg ggacgactga gaacggcgag aatctaaccc ttttatattc atgtttatgc
      541 ctgcatccat gaatctcggc aactgctggc aggcagcgtg cactgttctc tgatagacaa
      601 catagaattg gcttcccggg gtggcccgat ccaacctcga ctggatctgg ttctggggac
      661 caagtcaaac aaaagggggc acgcaccggc cccctttttt atttgagatg cgcggcgcgc
      721 tcgcatcgcg gtccacgact tttctgctag gcctcagggc ccaaaaaagt gagcggccgg
      781 tgggggtgcc ggccgctcgg gcgtggggtg atggagggta tcctgttacg agatcgagat
      841 caggaatcgc ctgactctcg accacgaaga gcattctccg tttgcgggtt gtgggcgtcg
      901 atagtcctac cggttcattc ggtccggtac ttcccagcgt tccgccagca gccctgggtg
      961 gtacttcggt acttgccggg ccgctatgct ctctcgtcgg actgagctat tctcctactc
     1021 cgatgcatga cctcagctac ttccgggacc acctggacat ttttggggat atggctcagc
     1081 ggcgcggact tggcctcgac ctgggcgctt ttcgcgtgct cgatcaagag cgccggcagt
     1141 tgattacccg gacggagcaa ctcaaggccc agcgcaataa ggccagcgag gagatcggcc
     1201 gcctcaagaa ggaaaaacag gacgccggcc cgttactggc ccaaatgaag caggtctccg
     1261 atcaaatcaa gcaggcggat gaccgcatct cgcagttgga ccagcaactg cgcgacttca
     1321 tgctgacggt cccaaacctg ctgcattcct cggtcccgcc gggcaaaacc tccgccgaaa
     1381 acgttgaagt gcgccgctgg ggttcgccgc cgaagttcga tttcgtgccc aggccgcact
     1441 gggagatcgg cgaaaacgca gggattctcg atctcgcaag agccgtgaaa atcgcaggag
     1501 cccgctttgc tttgtaccgc ggcgcgggcg cgcgcctgga acgtgccctg gcgaacttct
     1561 tcctggatgt acacacgaag gaacactcct acacggaggt tcttccgcct tttcttgtca
     1621 atacggcttc actcgtggcc gacggcaagc tgcccaagtt tgccagtgac atgttccata
     1681 tcgagggcag cgatttctgg ctcaccacca cagcggagac agaattgacc aacctcttcc
     1741 gggacgaaac tctggacgcc gacgccctgc cggtccgagt gtgcgcctgg acctcgtgct
     1801 tccgttccga agcgggcgcg gccggaaaag acacgcgcgg catcaagcgc cagcaccaat
     1861 ttcaaaaggt cgaactgttc aagttcacgc ggccggaaca gagctatgaa gagctggagc
     1921 gtcttgtggg caacgccgaa gccattctgc agaggctcgg cattcactac cgagtgatgc
     1981 agctttgcgc gggggacacg ggattcgcct cgtccaagac atacgacatc gaagcatggc
     2041 ttccttcatc ctccgaattc atggaaattt cgtcttgttc gaattgcgag gcgttccagg
     2101 cgcgccgggc cgggatccgg atgaaaagca agggaaccaa gagcgagttt gtgcatacgc
     2161 tgaacggctc cggcttgccg gttggacgca cctggatcgc gatcgtggag aactgccagc
     2221 gggccgacgg ctccgtcgtc atcccggaag tgctgcgccc gtacatgggg atggaccgaa
     2281 ttcccgccct tgccggttcc gaggcctgaa aatgccttga gcggcgtttt ttgggctaac
     2341 tctaatcaaa caaaacactt atcaaaccat cggcccggtc tgggtgctgc cgtgcttgcc
     2401 ccatacgtgc cgcaaccctt tgaatttgca cgttttcgtt tgacacccct ttttccgctt
     2461 gcatacactg cgctcacccg tggttcaagg gggatatcaa ggctagcctc tccacggaac
     2521 aacaacaatc gaatgacctt ttttgcgcgc ttggcgcggt tcctgttctg gttgctcatc
     2581 gtgtcctggg gcgttgcgct cctgcgccgc atcgcggcct gggcgctgca gcgctctgct
     2641 cctcacgatg tggatgagcc cgcgcaggcc tccgtggcgc ggaggctgca ccgcgacccg
     2701 gtttgtggaa cccacgtttc cgaccagatt tcggtcccgc tgctcgaggc gggggaaaca
     2761 ttgcattttt gttctgagga gtgccggacg aagtacgcga acttgcaccg ccccgccgtc
     2821 aacgaatagg cgggagcgac acccttttca cggaaaggaa tcaactagat ggctacggtt
     2881 gcaaaaccca ctgccccgcc gatggtgccg aggatcgccc cgcgcgagga tctcaatcct
     2941 ttccgcatcg ctcaaatcca gttcgacatg gcggcggaat tcctgaagct ggaccccagc
     3001 cttcgccaga tcctgcgaac gcccaagcgc gtgctcgagg tctccattcc gacgaagatg
     3061 gacaacggcc aagtgaaggt gctcaccggc taccgcgttc agcacaacgt ggcgcgcggg
     3121 ccgggcaaag gaggcatccg ctaccatccc ggcgtcaccg tagatgaagt caaggctctg
     3181 gctgcctgga tgacctggaa gaccgccacg gtcaatgttc cgttgggggg cgccaagggc
     3241 ggtgtgattt gcgaccccaa gcgcatgtcc aaaggcgaac tcgagcgcat gacccggcgg
     3301 tacgccagcg aaatcctccc catcatcggg cccgaccaag acatccccgc tccggacgtc
     3361 tacaccgact cccagaccat ggcttggatt atggacacct attccatgac catcggccgc
     3421 aatgcccatg cggtggtgac gggcaagccg ctttccctgg gcgggtcggc ggggcgcgac
     3481 gaagccactg cccgcgggct catctatgta gt
//
LOCUS       DASPUL010000162         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1533005, whole genome shotgun sequence.
ACCESSION   DASPUL010000162 DASPUL010000000
VERSION     DASPUL010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533005"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VEJ00_03965"
     CDS             complement(<1..273)
                     /locus_tag="VEJ00_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00348.1"
                     /translation="MPVTIADMQSPETALLAKESRLDSVLRQLGRVLVAYSGGVDSAY
                     LAWAAHRALGKNMLAVIADSASLARTQLADALAFAGEQGFAVEVIAT"
     gene            complement(302..1057)
                     /locus_tag="VEJ00_03970"
     CDS             complement(302..1057)
                     /locus_tag="VEJ00_03970"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXX00349.1"
                     /translation="MRSSAAVLCRDESSLQTLKAGLSECGMDLVPCPSREQALELAVA
                     RRCSTLIVDFDLPGAEEVIRMAALLPDTQKPVLIALASRAWPGTGEAFHSGASRILYR
                     PLDVAELKNALKTGKKNARKSQRKSPRYEMKTLVYLELDHLTLPSISINIGEHGLAVQ
                     ATEAIPMSSRLLFRCVLPGTDFTLQGYADVIWASDQGRAGLFFSELGPAARKHLRRWL
                     SRRSAFEKNHHKKSTHDVHDLLPPLDANVSFAE"
     gene            complement(1141..1746)
                     /gene="msrB"
                     /locus_tag="VEJ00_03975"
     CDS             complement(1141..1746)
                     /gene="msrB"
                     /locus_tag="VEJ00_03975"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXX00350.1"
                     /translation="MFDSELDRDASKVSRRVFLTGAGAAVAGFAFWQLRKPRVLEAAA
                     AAAASEPKDVTVVLFSDSGERLKKVQLPKVIKTEAEWRKQLSDNAFDIARRADTEIAF
                     TGKYWNVHDKGLYRCICCDNALFDSATKFDSGTGWPSFWQPIAAENVTETRDTTFGIV
                     RTAVSCTECDAHLGHVFDDGPQPTGLRYCMNSASLRFIPQS"
     gene            complement(1755..>2267)
                     /gene="msrA"
                     /locus_tag="VEJ00_03980"
     CDS             complement(1755..>2267)
                     /gene="msrA"
                     /locus_tag="VEJ00_03980"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXX00351.1"
                     /translation="GCFWGIQAVFQHVKGVISATSGYSGGSAKTAQYEVVSTGETGHA
                     ESVQIVYDPSQITYGELLRVFFSVTHDPTELNRQGPDDGTQYRSVIFYNGDEQKRIAE
                     AYIAQLDQAKVFKHKIVTQVVPLQTFYPAEAYHQNYAVLHPNQPYIVFNDAPKVEHLR
                     QQFPDFYVSK"
BASE COUNT          442 a          648 c          727 g          450 t
ORIGIN      
        1 ggtggcgatg acttcgacgg cgaatccctg ctcgccagcg aaagcgaggg cgtcggcgag
       61 ttgcgtgcgt gccaggctgg cagaatccgc gatgacggcg agcatgttct tcccgagtgc
      121 acgatgagcc gcccaggcca ggtacgccga atcgactccg ccggagtagg ccacgagcac
      181 gcggcccaac tggcggagaa ctgagtcgag ccgggattcc ttcgccaaaa gcgccgtctc
      241 aggcgattgc atgtccgcta tggtaacagg catggccgcc gggcgcagga cgacccgctc
      301 cttattctgc gaacgagacg ttggcgtcca gaggaggcag tagatcgtgc acgtcgtgcg
      361 tgctcttttt gtggtggttc ttttcgaaag cgctgcggcg actcagccag cggcgcagat
      421 gtttgcgggc cgcggggcca agttcagaga agaataaccc ggcgcggccc tggtcgctgg
      481 cccagatcac gtcggcgtat ccctgcaggg tgaagtcagt tcccggcagc acgcaacgaa
      541 aaagcaggcg agagctcatg ggaatagctt ccgtcgcctg cacggccaag ccatgttcgc
      601 cgatgttaat gctgatactg ggcaaggtga gatgatcaag ctcaagatag accagcgttt
      661 tcatttcgta gcgcggagac ttgcgctgac tcttgcgcgc gttcttcttg cccgtcttga
      721 gcgcattttt gagctctgcg acgtcgagag ggcgataaag gatgcggctg gcgccggagt
      781 ggaacgcctc gcccgtcccc ggccaggcgc ggctggccag ggcaatgagg acaggcttct
      841 gggtgtcagg caacagcgcc gccatgcgaa tcacttcttc cgctccggga agatcgaaat
      901 ccacgatgag tgtggagcag cggcgggcaa cggcgagttc cagcgcctgc tcgcgggatg
      961 gacacggaac caggtccatg ccgcactcgc tcaggccggc cttcagggtc tggagcgacg
     1021 actcatcgcg gcacagcact gcagccgagg aacgcatgaa cacaaactac gtgccagcac
     1081 gtaaccgcga aagtcacacg agtaaccgca gagttggtta cccgagacta cttgcgcgct
     1141 tcagctttgt gggatgaacc gaagcgacgc cgagttcatg cagtagcgga gtccggtcgg
     1201 ctgcgggccg tcatcgaaaa catggccaag atgagcgtcg cattcagtgc aggaaacggc
     1261 ggtgcgaacg atgccgaacg tggtgtcgcg cgtttcggtg acattctccg ccgcgatcgg
     1321 ctgccagaag ctgggccagc ccgtcccgga gtcgaatttc gtggcggagt cgaacaacgc
     1381 gttgtcgcag cagatgcagc ggtagagccc cttgtcgtgc acgttccagt atttgccggt
     1441 gaaggcgatt tctgtgtcgg cccggcgcgc aatgtcgaat gcgttgtcgg acagttgttt
     1501 gcgccactcg gcctcggtct tgatcacctt cggaagctgc actttcttca ggcgctcgcc
     1561 ggagtcggaa aacagaacta ctgtgacgtc cttcggttcg ctcgcggcag cggccgcagc
     1621 agcttccagt acgcgcggct ttctcagttg ccagaatgcg aatccggcaa ccgcggctcc
     1681 tgcgcccgtg aggaataccc tgcggcttac cttcgatgcg tctctgtcaa gctccgaatc
     1741 aaacatatcc cctcctactt cgagacgtaa aagtcgggaa actgctggcg caggtgctcg
     1801 actttaggag cgtcgttgaa aacaatgtag ggctgattgg gatgcagcac ggcgtagttc
     1861 tggtggtagg cctctgccgg atagaaagtc tgcagcggca cgacctgggt gacgatcttg
     1921 tgcttgaaaa ccttcgcctg atcaagctgg gcgatgtaag cctcggcaat gcgcttctgt
     1981 tcgtcgccgt tgtagaagat caccgagcgg tattgcgtgc catcgtcggg gccctggcga
     2041 ttgagttccg tcgggtcgtg ggttacggag aagaatacgc gtagcagctc tccgtaagtg
     2101 atctgcgacg gatcgtagac aatctgcact gattcggcat ggccggtttc tccggtgctg
     2161 acgacttcat attgggcggt cttggcggag ccgccggaat agcctgaagt cgcgctgatc
     2221 actcccttca cgtgctgaaa gacggcctgg atgccccaga agcatcc
//
LOCUS       DASPUL010000163         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1128062, whole genome shotgun sequence.
ACCESSION   DASPUL010000163 DASPUL010000000
VERSION     DASPUL010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128062"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..792
                     /locus_tag="VEJ00_03985"
     CDS             109..792
                     /locus_tag="VEJ00_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00352.1"
                     /translation="MKPRNAFLPLMLTLLAAASHAQTQTSKPEDAKATGGMPAITAAR
                     CAYTPNDGECAAPNHPSDAAREPAANTTVAQIPRRMPRRPTPRHHAAYMGPYPSNWMS
                     EGNTRGAVIGTLIGGGLGIALGIRVNTDNHAQAKVAAPLLIGGLGALLGAVAGGNWPA
                     APHWRHHHGWPDGDDEEAVASRSHALSQSAQNAPDRMAPQKPASPKLDAPELSGNARK
                     SVPVLTALP"
     gene            980..>2400
                     /locus_tag="VEJ00_03990"
     CDS             980..>2400
                     /locus_tag="VEJ00_03990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXX00353.1"
                     /translation="MARSSRRSLFLVVSFLLACGFLGILFAQRTGQQSSMASDSEVKD
                     SFKQFADVYSIVEENYAEPVNADKAIYNGAIPGMLHALDPHSNFFDPKNYALMREDQR
                     GKYYGVGMTVGPRNNKVIVIYPFVGTPAYKAGIHPGDVIIAVDGKSTENMSTGDVADM
                     LKGPKGTTVHISISREGVEKPMEFTLVRDEIPRYSVDVHFMIRPGVGYMHVNGFNETT
                     EHEVGEALDQFGDLKGLILDLRGNPGGLLSEGVGVADKFLKKGQLIVSHHGRASAEKR
                     YIAQHGNGGKDYPIVVLVNRLTASAAEIVSGAIQDHDRGLIAGEVTFGKGLVQTVYPL
                     SENTGLALTTAHYYTPSGRLIQRDYSNVSLYDYYYNRDSAETSNTANREVKLTDSGRT
                     VYGGGGITPDVNIPPVKTNHFQDTLLQHYAFFNFAKRYVVSHHPTKNFEVDDATMQEF
                     RKSLDEADIAYTQADLIDNNDWL"
BASE COUNT          523 a          793 c          659 g          425 t
ORIGIN      
        1 taacacttga agtttgaccg accgcactac gcgattctcc cctgtgacag tccagaaacc
       61 gtcccaaagc gcccgacgcc acttcagcct taaatctacg atgcactcat gaaacctaga
      121 aacgcatttc tccccttgat gttgacgctg ctggcggccg cctcgcacgc tcagacgcag
      181 accagcaagc ctgaagacgc aaaagccacc ggagggatgc ccgccattac cgccgcccgc
      241 tgcgcctaca caccgaacga cggcgaatgc gcggccccga atcaccccag cgatgccgca
      301 cgcgagccgg ctgccaacac gaccgtggct caaatacccc ggcgtatgcc tcggcggcca
      361 acgccgcgac accatgcggc ttacatgggg ccctatccca gcaactggat gagcgaggga
      421 aacactcgcg gtgccgtcat cggtaccttg atcggaggtg gattgggaat cgcattagga
      481 atccgagtaa acaccgacaa ccatgctcag gcgaaagtcg ctgcaccttt gcttatcggg
      541 ggtctgggcg ctctcctcgg agccgtagcc ggcggcaact ggcccgcagc gccacattgg
      601 aggcaccatc atggctggcc ggacggcgac gatgaggaag cagttgcctc cagatcgcac
      661 gctctctccc agtctgctca aaacgccccc gaccggatgg caccccagaa gcccgcgtca
      721 ccgaaactag acgctcccga gttgtcaggc aacgcgcgaa agtccgtccc tgttctgact
      781 gccctgccct gaatcctgcg agttaggcga tcctgaggaa cccctgccgc tcccgccgca
      841 tctcataaag agcgccctct tcggggtggt taccaacggt gaccttcccg gttcaggcta
      901 gtctaacggg gtggtatact gcctgtattc agcgcggtag ctggggtggc ggtaagccct
      961 caagcaaagg aaaacctaga tggctcgcag ttctcgccgg tctctcttcc tggtagtttc
     1021 ttttcttctc gcctgcggtt ttctgggcat cctgttcgcc cagcgcaccg gccagcagtc
     1081 gtccatggcg agcgactccg aggttaagga cagcttcaag cagttcgccg acgtttattc
     1141 catcgtcgag gagaactacg ccgagcccgt gaacgccgac aaggccattt acaacggcgc
     1201 catccccggc atgctgcacg cgcttgatcc gcactcgaac ttcttcgatc caaaaaacta
     1261 cgcgctcatg cgcgaggacc agcgcggcaa gtactacggc gtaggcatga cagtcggccc
     1321 gcgcaacaac aaagtaatcg tcatctatcc cttcgttggc actcccgcct ataaggccgg
     1381 cattcatccc ggcgacgtga tcatcgctgt tgatggcaag tccacggaga acatgagcac
     1441 gggcgacgtc gccgacatgt tgaaggggcc caaaggcacg acggtacaca tctccatatc
     1501 gcgcgaaggc gtcgagaagc cgatggagtt cacgctggtc cgcgacgaga ttccgcgcta
     1561 ctcggttgac gtgcatttca tgatccgtcc cggcgtcggc tacatgcacg tcaacggctt
     1621 caacgagacc accgaacacg aagtaggcga agcgctcgac cagttcggcg atctgaaggg
     1681 cctgatcctg gacctgcgcg gcaatcccgg cggcttgctc agcgaaggcg tgggcgtggc
     1741 cgacaagttc ctgaagaagg gacagctcat cgtttcgcat cacggccgcg cgtctgccga
     1801 gaagcgttac atcgcgcagc acggcaacgg cgggaaggat tatccgatcg tcgtattggt
     1861 caaccgcctg acggcgtcag cggcggaaat tgtttccggc gcaatccagg atcacgaccg
     1921 cggcctgatt gcgggtgaag tcacgttcgg caagggcctg gtgcagacgg tctatccgct
     1981 ctcggagaac accggcctgg ctctgaccac ggcgcactac tacacaccga gcggccgcct
     2041 catccagcgc gactactcga acgtttccct ctacgactac tactacaatc gcgacagcgc
     2101 cgaaacttcg aacacagcca accgcgaagt gaaactgacc gacagcggcc gcaccgtcta
     2161 cggcggcggc ggcattacgc ccgacgtcaa cattccgccg gtcaagacca atcacttcca
     2221 ggacacgctg ctgcagcact acgcgttctt caacttcgcc aagcgctacg tggtcagcca
     2281 ccatcccacg aagaactttg aagtcgacga cgcgacgatg caggaattcc gcaaatcgct
     2341 cgacgaagcc gacatcgcct atactcaggc cgatctgatc gacaacaacg attggctcaa
//
LOCUS       DASPUL010000164         9622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_304099, whole genome shotgun sequence.
ACCESSION   DASPUL010000164 DASPUL010000000
VERSION     DASPUL010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9622)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9622
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304099"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1503
                     /locus_tag="VEJ00_03995"
     CDS             238..1503
                     /locus_tag="VEJ00_03995"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF003705.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HXX00354.1"
                     /translation="MEAPPGFLAEALNAPDAQDGTLAPIVRNAPSTVEQLTARFYARF
                     GTSARIFRVPGRVNLIGEHTDYNDGFVMPAAIGFYTWVAGGCRPDRALTAYSELYDEE
                     TTVSLDDLSGPPRQHWSDFIRGLAATLLEAGYKLAGANLLIHGDVPVGAGLSSSASLE
                     VAVALALTQISSVTPSRLELVKICQRTEHQYAGTRCGIMDQFAVSFAAAQHALMLDCR
                     SLEYRLLPVPPDLRLVVCNSMVRRELASAEYNLRRSDCEAGVRLLQSHMPAIAALRDV
                     EMKDLDRCAELLPERVYRRCRHVVSENSRVLKAASALEANDADHFGELMYQSHASLRD
                     DYEVSCKELDLLVELASRVPGVYGARMTGGGFGGCTVNLVRAAEAASFQKQIAHEYSK
                     QTGIMPSVHVCEITQGAEALPAAGSPALK"
     gene            1500..2561
                     /locus_tag="VEJ00_04000"
     CDS             1500..2561
                     /locus_tag="VEJ00_04000"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:NF008724.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HXX00355.1"
                     /translation="MKPKTLYAEPHRRFNPLTREWILVSPHRTQRPWSGQLETAQASP
                     SLAYDPECYLCPGNQRAGGARNPDYGSTFVFINDFAALKPEVQPARLDESGRGLLLSE
                     SEAGICKVVCFSPRHDLTLARMTVAEIAAVVDLWANEFAAIASLPYINYIQIFENRGE
                     IMGCSNPHPHGQIWANQTLPDEPRKEQESQQAYARQHGACLLCDYLQLETQATSRMVA
                     ENAHFAALVPFWAVWPFEILLISKAHAPNLVSLDPAMRESLADILKQITTRYDNLFDV
                     PFPYSMGFHQAPTDDEAHPEWHLHAHFYPPLLRSATIRKFMVGYEMLASPQRDLTPEA
                     AAAILKNLPATHYADRKPR"
     gene            2585..3433
                     /locus_tag="VEJ00_04005"
     CDS             2585..3433
                     /locus_tag="VEJ00_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HXX00356.1"
                     /translation="MKVYLVDGTYELFRHYYALPSARDEDGREVAAVRGVLASVLGMV
                     KEGATHIGVATDHVIESFRNDLWPGYKTSDGIEPDLWAQFPLLEEILSAAGIVVWPMI
                     EYEADDALAAAAVAAARDARVEQVLICTPDKDLAQCVRASRIVQLDRRKRAIIDEAGV
                     VRKFGVSPESIPDYLALVGDSSDGYPGLPGWGAKSSAAVLARFRHLEGIPKDWHEWGI
                     KVASAGALADTLSREWDRAVLFRKLAILRTDIPLFQDVEELRWSGPTPSFDELAARLD
                     RARTVR"
     gene            3600..6206
                     /locus_tag="VEJ00_04010"
     CDS             3600..6206
                     /locus_tag="VEJ00_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00357.1"
                     /translation="MRQSPAAILALWFFTLLLAATTVAAQAQSCCTALNTLKGEGKFT
                     GQISTDPDGSSMQVSYSDQSVLVHYTSSVTVCQGGQPASVSALVRGASVSVFGPMRRN
                     GTSMEIDAARIFVAGRLRTNPAPSVPNAQPTQSGTTGQLPPPAAKPNPEPALPNAQQT
                     QPGTSGPRPTPNLAILRGGTHAETMQRLRVVRTYHLAELRANSKVTVGEAKLDFRPML
                     TNPKALFNIAQNLHAMPQHVQVTEETSEASEIEQGLVIHHVLSYHILPGKCGDANVKA
                     QLDRAGAACFTRGSASERIAEFSKPGSPRYVADPGKRQTAIAAYQRNSALQDADATKH
                     IAELRKALADPTQRAAIVAKIGQAEAARLPTLSDDQLKEEMINSAEQRFEETMYVPKL
                     DSANYLHGQHNLSIAASPAEMAAAKQLLREGVHGGSPSSFPKLLKIVPSKHAGLTKTP
                     EADKVADLDFGPYIYLTGFTLGRDHEWKWGASTTVNWCVVGCSATYGFDLWAGFGYGL
                     GLRFPIQTDLKYHVVVHANNSAEASLTPTFKPIQGTFNDFANSGLSVDQIFNAQELVA
                     EAQAHAGFDINLPGLNPSDDYKIGKDLTEDLPAPFTNGTFTPPAPGVPSPPGQTIFDQ
                     IDLLGGLLNFGVVGGQLFPALEITLFSNALRFTLDDEITNRSTTVASSGKPMSLGVGP
                     TNGGDQSHFSFGDPVYNIGFGMTPGVAPNVFVDIGIWSHNWNFPIWFPELTVDLPPGG
                     QDFSCHAGTTCVLDFQPVYNASTGQDKNMKQEMDVADRTLTSSGCQRVNNQEGNYLCP
                     VKGMLGLCKAMLSNGAVTSCGPLVPNVVDEILKRGHCTDGDANYVCPSGMMGLCNDYL
                     KNQEILSCTQAK"
     gene            complement(6388..8124)
                     /locus_tag="VEJ00_04015"
     CDS             complement(6388..8124)
                     /locus_tag="VEJ00_04015"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXX00358.1"
                     /translation="MKKRSRSRREFLGLTGASVAGLASGSFVAAKVLAQTENAEINPR
                     DADLVVFNAKVYTVDPRSPRAEAFAVKGGRFAFVGSSAEARAFIGKGTRTFDSQQMTI
                     VPGFIDCHNHAGGNTLLYDVIVGNPYEVEFVTIASIIEKLRARAQKTPAGFWVDGYFF
                     DDTKVKDNRQLNIHDLDQVSQDHPVCVHHRGGHTSYYNSKALELAGINKHTPNRMGGT
                     YDHDENGELTGRVTDRAREVFIDVGKRETFTEEQKEQRDRDGLAYISKQFVRYGLTSV
                     HHEGGDLFALQQVRARGELLHRVSFETSGEILEAMIKNGISTGFGDEWIRFGATSEHT
                     ADGSFSERTMALSKPYAGTNPPYSGNITETQEDLNIWAERVQRAGIQPNCHANGDVAI
                     DMVLKAYERAQQVAPRPDVRPKITHCTLINEDLLRRIKALGAVPAPFTTYAYYNTDKF
                     HFYGEERMKWSMAYRSFLDAGIRAAAGSDFSPGPFAPLMGIQGMVTRTGWNGETWGAN
                     QRISLDEALQVNTINGAYNSHEEAIKGSITPGKLADFVVLADDPHTVSADRIKDIAIV
                     QTVTGGMTVYQK"
     gene            complement(8154..>9622)
                     /locus_tag="VEJ00_04020"
     CDS             complement(8154..>9622)
                     /locus_tag="VEJ00_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00359.1"
                     /translation="GYGGTQSLEAPHHYSKVAFGLLQELGIEVDRFHKYFDQKLYKGM
                     GLKRATFFDKETFGIDRLLPGFGTHYLGLTYPPEVLAQIPISDAARKDLERLQETKVD
                     YLPGLSTGEKRRKLIKTSYKDYLLQYVKVHPDVIKIFQTAPHGLYAVGIDAVSAWDCF
                     EMDYPGFAGMKLEGREDSPEQAEPYIFHFPDGNASVARLLVRSLVPGSVPGTTMEDVV
                     TSRVNYGRLDDSEGTVRIRLNSTAVHAQHVGDPHDAKQVEVTYVRGGKAHKVRAAHCV
                     LACYNMVIPYLCPEMPEKQKEALRYCVKLPLVYTNVQIRNWESFQKLGVSQIYSPGGY
                     FNGATLDFPVSIGDYRFPSKPEDPCLLHLLRTPCKPGLPAKEQYRAGRYELMTTPFEV
                     FERNIRDQLGRMLAGGGFDSARDVQGITVNRWPHGYAYEYNPLFEPLDRPDSERPCVI
                     GRQAFGRIHIANSDADGHAYTNIAIDQGYRAVREITAG"
BASE COUNT         1934 a         3000 c         2729 g         1959 t
ORIGIN      
        1 caccgtggtg agttgcccat gctgcatggc cgggatgacc ttccgcactc cgatcgaagc
       61 cgtactgaga acaccccagc gaagttttcg atccacgtta ttggtattca aatcacccac
      121 ccccatggga acctgccaca aaaggaccac gaatttccgc aaagaaatcg tttgctgaaa
      181 tcgctcggcc cgtttcctgg tttgcttatt ttaacctgtg cctttcgcag attacccatg
      241 gaagccccgc cgggcttcct ggccgaagcg ctcaatgcgc ctgacgcgca agatggtaca
      301 ttagccccaa tcgtgcggaa tgccccgtcc accgtcgagc agctaactgc gcggttttac
      361 gcccggtttg gaaccagcgc ccgcatcttt cgcgtgccgg gacgggtcaa cctgatcggc
      421 gaacatacag attacaacga cggttttgtc atgccggcag caatcgggtt ttacacctgg
      481 gtagccggag gatgccgtcc ggatcgtgcc ttgaccgcat attccgaact gtatgacgaa
      541 gaaactaccg tatcgctcga cgatctttca ggaccgccgc gacagcattg gagcgatttc
      601 attcgtggcc tagccgcgac cctgctggaa gcgggctaca agctcgcagg agccaatctc
      661 ctgatccacg gagatgtccc ggtcggagca ggactcagct cttcggcgtc cctggaggtc
      721 gcggtggctc tggccctgac tcagatctcc agtgttacgc cgtcgcgcct ggaactggtg
      781 aagatctgcc agcgcaccga gcatcaatac gccgggacgc gctgtggaat catggaccag
      841 ttcgcggttt cattcgccgc cgcgcagcat gcgctgatgc tggactgccg ctccctcgag
      901 tatcggttgc ttccggtgcc gccggatctg cgtctcgttg tctgcaacag catggtccgg
      961 cgcgagctgg cttcggcaga atacaacctg aggcggagtg actgtgaagc tggagtgaga
     1021 ctgctgcaat cccatatgcc cgccattgcc gccttacggg atgtggagat gaaagatctt
     1081 gatcgttgcg ccgaattgtt gccggagcgc gtctatcgcc gttgccgcca cgtggtcagc
     1141 gagaactcca gagtgctgaa ggcagccagc gcgcttgagg cgaacgatgc cgaccatttt
     1201 ggagagttga tgtaccagtc tcatgccagc ctgcgcgacg actacgaagt cagctgcaag
     1261 gagctcgatc tgctggtcga gctggcttcc agggttcccg gcgtatacgg agctcgcatg
     1321 accggaggtg gatttggcgg atgtactgta aacctggtgc gcgccgctga ggccgcctcg
     1381 ttccagaaac agattgcgca cgagtactcg aagcaaaccg gcatcatgcc ttccgttcac
     1441 gtctgcgaga ttacgcaggg cgcggaagcc ttgccggctg cggggagtcc tgccctcaaa
     1501 tgaagccgaa gacgctttat gccgagcccc atcgccgctt taatccgctc acccgggaat
     1561 ggatcctggt gtcgccgcac cgcactcagc ggccgtggag cggacagctg gaaacggccc
     1621 aggcctcccc ctcgctggcc tacgatcccg agtgctacct ctgccccggc aatcagcgag
     1681 ctggcggcgc gcgcaatcct gattacggct cgaccttcgt cttcatcaat gactttgccg
     1741 cactcaaacc cgaggttcaa cccgcccgcc ttgatgagtc tggccgcggc ttgttgctgt
     1801 ccgagtccga agctggaatc tgcaaagtag tctgcttctc tccgcggcat gacctgactc
     1861 tggcgcggat gaccgtagcg gaaatagccg ccgtagtaga cctctgggcg aacgagttcg
     1921 ccgccatcgc atcccttccc tacatcaact atatccagat cttcgaaaac cgcggcgaga
     1981 tcatgggctg cagcaatcca catccccacg gacagatctg ggccaaccag acccttcccg
     2041 acgaacctcg gaaagaacag gagtcacagc aggcctacgc gcggcagcat ggcgcttgcc
     2101 tgctttgcga ctatctccaa ctggaaaccc aggctacttc ccgcatggtc gcggagaacg
     2161 cccacttcgc agctctcgtc cccttctggg cagtgtggcc gttcgagatt ctgctgatct
     2221 caaaggcgca cgccccgaac ctggtatccc tcgatccggc catgcgcgag agcctcgccg
     2281 atatcctcaa gcagatcacc actcgctacg acaatctttt tgacgtcccg tttccttact
     2341 caatgggctt ccaccaggcg ccgaccgacg atgaagcaca ccccgagtgg catctgcacg
     2401 cgcacttcta ccctcctctg ctccggtcag ctacaatccg caagttcatg gtcggatacg
     2461 agatgctggc ctctccgcag cgggacctta ccccggaagc agccgccgcg atcttgaaga
     2521 acctgccagc cactcactat gccgatcgca agccgcgctg agagccggcc actggagcgt
     2581 cagcttgaag gtttacttgg tcgacggcac ttacgaattg ttccgccact actacgcgtt
     2641 gccttccgcg cgtgacgagg atggacggga agtggctgcc gttcgcggcg tcctcgcctc
     2701 ggttcttggt atggtcaagg aaggcgcaac ccatatcggc gtcgccaccg atcatgtcat
     2761 cgaatccttc cgcaatgatc tgtggccggg ctacaagacc agcgacggca tcgagcccga
     2821 tctatgggcg cagttccccc tgctcgagga gatcctgtcc gcagcaggca ttgtcgtctg
     2881 gcccatgatt gaatacgagg ccgacgacgc gctggccgca gccgccgtgg cggccgcccg
     2941 cgatgcccgg gtcgagcaag tccttatctg cactcctgac aaggatctcg cccaatgcgt
     3001 gcgcgcgtcc cgcatcgtgc aattggatcg gcgcaaacgc gcaatcatcg atgaagcggg
     3061 cgtggtcagg aagttcggtg tttctcccga gtccattccg gactacctcg cgctggtggg
     3121 agattcgtcc gacggctatc ccgggctgcc gggctggggt gccaagtcct cagccgccgt
     3181 gctcgcccgg tttcgccacc tggaaggcat cccgaaagac tggcacgaat ggggcatcaa
     3241 agtggcgagc gccggcgccc tcgccgacac cctttctcgc gagtgggatc gagctgtgct
     3301 ctttcgcaag ctggctattc tgcgcaccga tattccgctg ttccaagatg tggaagaact
     3361 tcggtggagc ggtcccacac cctccttcga cgagctggcg gcacggctcg atcgagcgcg
     3421 caccgttcga taaaatccgg ggccccgcgc accacctgaa ccgagccaag acccgccacc
     3481 atggagaatt tccgaacagt ccactgcgga cttcgggccg aggtgtatcc gaaggtgccc
     3541 ccccgtcttc ttcggcatgt catttcgcaa tagaatcacg ctaaccatag gagaactccg
     3601 tgagacagtc ccctgcagcg atcctggcgt tatggttttt caccctcctg cttgctgcga
     3661 ccaccgtcgc agcccaggcg cagtcgtgct gcactgccct caacacgctc aaaggggaag
     3721 gaaagttcac gggacagatc tcgaccgatc ctgacggcag ctcgatgcag gttagctaca
     3781 gcgatcagag cgtgcttgtg cactacacca gctccgtcac ggtctgtcag ggcgggcagc
     3841 cggcctctgt tagcgctctt gtccggggcg cgagcgtttc cgtctttggt cccatgcggc
     3901 gcaacggcac aagcatggaa atcgacgcag cgcgaatttt tgtcgccggc cggctccgga
     3961 cgaatcccgc gccgagcgtg cccaatgctc aaccaacaca atcgggcacg accggccaac
     4021 tcccgccgcc agcagccaag ccgaatccag aacctgctct gcccaatgcc cagcagacac
     4081 aaccgggcac aagcggcccg cgcccgactc ccaacctggc gatcctccgc gggggcacgc
     4141 acgcggaaac catgcagcgc ctccgcgtcg tgcgcaccta ccacctggcc gaactgcgtg
     4201 ccaactcgaa agtgacggtg ggagaggcaa agctcgattt ccggccgatg ctgaccaatc
     4261 ccaaagcgct cttcaatatc gcgcaaaacc tgcacgcgat gccccagcat gtgcaggtca
     4321 ccgaggaaac gtcagaagcg agcgagattg agcagggact ggtgatccac cacgtcctca
     4381 gctaccacat ccttccggga aagtgcggcg acgccaatgt gaaggcgcag ctggatcggg
     4441 ccggagccgc gtgcttcacg cgtggttcgg cgagcgaacg catcgccgaa ttcagcaagc
     4501 cgggcagtcc gcggtatgtg gctgatcccg ggaagcggca aacggctatc gcggcctacc
     4561 aacgcaacag cgcgcttcag gatgccgacg caaccaagca cattgccgaa ctgcgcaaag
     4621 cactcgcgga tccgacgcag cgcgcggcga tcgtcgccaa gattggccag gccgaagctg
     4681 cgcgcctgcc cactctcagc gatgaccagc tcaaggaaga gatgatcaac tcagcggagc
     4741 agcggtttga agagacgatg tacgtgccga agctcgactc ggccaattac ttgcacggcc
     4801 aacacaacct ctcgatcgct gccagccccg cggaaatggc ggcggccaag cagttgctgc
     4861 gcgaaggcgt gcacggtggc agcccatcca gtttccccaa actcttgaag atcgttccga
     4921 gcaagcatgc aggccttacc aaaacccctg aggccgataa ggtcgccgac ctcgactttg
     4981 ggccctacat ctaccttacg ggattcacac tcgggcgcga tcatgaatgg aaatgggggg
     5041 ccagcaccac cgtcaactgg tgtgtcgtag gctgctcagc aacctacggc ttcgacctct
     5101 gggccggctt cggatacggc ttaggcttgc gctttcccat ccagaccgac cttaagtatc
     5161 acgttgtagt tcacgcgaac aattcggccg aggccagtct gacgccgacg ttcaagccga
     5221 ttcagggcac gttcaacgat ttcgccaact ccggactttc cgttgaccag atctttaacg
     5281 cgcaggaact ggtggcggag gcacaagctc acgccggctt tgacatcaat ctcccgggcc
     5341 tgaaccccag cgatgactac aaaataggca aggaccttac cgaggatttg cctgctccgt
     5401 ttacgaatgg cacattcacg cccccggctc ccggcgtgcc aagccctccg ggccaaacca
     5461 tctttgacca gatcgacctg ctcggcgggt tgctcaactt cggcgtggtc ggcgggcagc
     5521 ttttccccgc gctggagatt acgcttttct ccaacgctct ccgtttcacc ctcgacgacg
     5581 agatcacgaa ccggtcgacg acggtcgcct cgagcggaaa gccgatgtcg ctcggtgtcg
     5641 gcccgaccaa tgggggagac cagagccact tcagcttcgg ggacccggtc tacaacatcg
     5701 gctttggcat gacgccgggc gtggccccga acgtcttcgt cgacatcggg atatggtcgc
     5761 ataactggaa cttccccatc tggtttcctg agcttaccgt tgatcttccg cccggtggcc
     5821 aggatttcag ctgccatgcc ggaaccacgt gtgtgctcga tttccagcct gtatataacg
     5881 cgagcaccgg gcaggacaag aatatgaagc aagagatgga cgtggccgac cggaccctga
     5941 ccagcagcgg ctgccagcgc gtgaacaacc aggaaggaaa ctatctttgt ccggtcaaag
     6001 gcatgctcgg attgtgcaaa gccatgctga gcaatggcgc ggtgacaagt tgcggcccac
     6061 tcgtaccgaa cgttgtggac gagatcctca aacggggaca ctgcacggac ggcgatgcca
     6121 actatgtctg cccatccgga atgatgggcc tgtgcaacga ctatctcaag aatcaggaga
     6181 ttctctcttg cacgcaagca aaataacttg agtgcaatcg ggaattccag ccaagacgtg
     6241 cgggtcgact cgataatgat tcgccgtgaa ggccctgttc acgggaatca gtcgcggtgc
     6301 caaccttatt ctggacgtga gtaccccagc cgcggatcac ggtcgaagcc cacagcagcg
     6361 tcgtcggcca caacccaaca aacctggcta cttctggtag accgtcatgc caccggtaac
     6421 cgtctgcacg atcgcgatgt ctttgattct atccgcgctt acagtgtgag gatcgtcggc
     6481 cagaacgacg aagtctgcca gcttgccagg cgtgatcgat cccttaattg cttcttcgtg
     6541 tgagttgtac gcgccgttga tagtgttcac ttgcagggcc tcatctaggc ttatgcgctg
     6601 gttcgctccc caggtttccc cattccagcc cgtgcgcgtg accattcctt ggattcccat
     6661 cagcggggcg aacggcccgg gactgaagtc tgagccagcc gccgctcgaa tgccggcatc
     6721 gaggaaggaa cggtacgcca tgctccactt catgcgttct tccccataga agtgaaactt
     6781 gtcggtgttg tagtacgcat aggttgtaaa gggtgcagga actgcaccca gcgccttgat
     6841 acggcgcagc agatcctcat tgatgagagt gcagtgcgtg atcttggggc gcacatctgg
     6901 gcgcggcgcg acttgctgcg cccgctcata cgctttcaac accatatcga tcgcgacatc
     6961 cccgttagcg tggcagttag gctggattcc agcccgttgc acgcgctccg cccagatgtt
     7021 gagatcctcc tgcgtttcgg tgatgttgcc gctgtaagga ggattcgtgc cggcataggg
     7081 cttgctcaac gccattgtgc gctcggagaa ggagccatcg gccgtgtgct cggaagtggc
     7141 gccgaagcga atccattcgt ctccgaagcc ggtgctgatt ccattcttga tcatagcctc
     7201 cagaatttcc ccgctggtct cgaagcttac tcggtgcagg agttcgcccc gggctcgcac
     7261 ttgctgcagc gcaaacagat ctcctccttc atggtgaacg ctggtcagac cgtaacggac
     7321 gaactgcttg gagatgtatg ccagcccgtc gcgatcgcgc tgttccttct gctcttccgt
     7381 gaaagtctcc cgtttgccaa cgtcgatgaa tacttcgcgc gcgcggtcgg tcactcgtcc
     7441 cgtaagttct ccgttttcgt cgtggtcgta agttccgccc atgcggttcg gggtgtgctt
     7501 gttgattcca gccagctcca gcgctttgct gttgtagtaa gaagtgtggc cgccacgatg
     7561 atggacgcaa accggatggt cctgcgaaac ctggtccagg tcgtgtatgt tgagctggcg
     7621 attgtccttc accttggtgt cgtcgaagaa gtatccatcg acccaaaacc ccgcgggcgt
     7681 tttctgggct ctggcgcgta gtttctcaat gatgctggcg atggtgacaa actcgacctc
     7741 atagggattg ccgacaatga catcgtagag cagcgtgttg cctccagcgt ggttgtggca
     7801 atcgatgaat ccgggcacga tcgtcatctg ctggctgtcg aaggtccgcg ttcccttgcc
     7861 gatgaacgcc ctggcctctg cggaacttcc cacaaaagca aatcgcccgc ccttcaccgc
     7921 gaaagcctcc gccctcgggc ttcgcggatc gaccgtatac accttcgcgt tgaaaaccac
     7981 cagatcggca tcgcgcggat tgatctccgc attttccgtc tgcgccagca ctttggcggc
     8041 cacaaaacta ccgcttgcaa gaccggccac gctggctccg gtcaagccca gaaactcccg
     8101 acgacttcgg ctgcgcttct tcatccctct ccccacggcg cggcttcgtg cttctagcct
     8161 gccgtaatct cccgcacggc gcggtaaccc tggtcaatcg cgatattggt gtaagcgtga
     8221 ccgtcagcat cagaattcgc aatgtgaatg cggccgaaag cctgcctgcc gatgacgcaa
     8281 ggccgttcgg aatcgggacg gtctagaggc tcgaacagcg ggttgtactc ataagcatag
     8341 ccgtgcggcc atcggttcac agtaatcccc tgaacatcgc gagcggaatc gaatcctccc
     8401 ccggcaagca tgcgtccaag ttgatcacgg atgttgcgct cgaagacctc aaagggcgta
     8461 gtcatcagct catagcgtcc cgcgcgatat tgctccttgg caggtaagcc aggcttacaa
     8521 ggcgtacgca aaaggtgcaa caggcaggga tcttctggct tgctggggaa acgatagtca
     8581 ccgatggaaa cgggaaaatc cagagttgcg ccattgaaat atccgcccgg cgagtagatc
     8641 tggctcaccc ccaatttctg aaaagactcc caattcctga tctgcacgtt ggtatagacg
     8701 agaggaagtt tcacgcagta gcgcagagct tctttctgct tctccggcat ttcgggacag
     8761 aggtaaggaa tgaccatgtt gtagcaagct agaacgcagt gtgctgcccg taccttgtga
     8821 gcttttcccc cgcggacata ggtaacttca acctgcttcg cgtcgtgtgg atcaccgacg
     8881 tgctgcgcgt gcaccgccgt gctgttcagc cggattcgca ccgtaccttc ggaatcgtcg
     8941 agacgtccgt agttcacccg cgaggtgacc acatcttcca tcgtggtgcc ggggaccgaa
     9001 ccgggcacca gggagcgcac caggagccgg gcgacagacg cattaccgtc ggggaagtga
     9061 aaaatatagg gctcggcctg ttccgggctg tcttccctgc cttccagctt cattcccgcg
     9121 aacccgggat agtccatctc aaagcagtcc caggcggata ccgcatcgat acccacggca
     9181 tacaagccgt gcggtgccgt ttggaaaatc ttgatgacgt cggggtggac cttgacgtac
     9241 tggagcaggt aatccttgta gcttgttttg atgagcttgc gccgcttttc tccggtggaa
     9301 agtccgggca ggtagtcgac ttttgtttct tgcagccgct ccagatcctt ccgcgcagcg
     9361 tcggagatcg gaatctgcgc gagaacttcc gggggatatg tcaaaccgag gtagtgcgtc
     9421 ccgaaaccag gcaagagacg gtcgatgccg aaggtttctt tgtcgaagaa ggtcgcacgc
     9481 ttcagaccca tgcccttgta gagtttctga tcgaagtatt tgtggaagcg atcaacctcg
     9541 ataccgagtt cttgaagcag accgaaggct actttgctgt agtgatgggg agcctcgagt
     9601 gattgagttc ctccgtaccc ga
//
LOCUS       DASPUL010000165         3887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_304251, whole genome shotgun sequence.
ACCESSION   DASPUL010000165 DASPUL010000000
VERSION     DASPUL010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304251"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VEJ00_04025"
     CDS             complement(<1..581)
                     /locus_tag="VEJ00_04025"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00360.1"
                     /translation="MNKPLLWRAGIIVATLLIFLFGIFGIPKSFSPQGMIASMGEHIH
                     LGLDLRGGTHLILQVQVNDAVNVDAQNAMEVLREQMRNRKIDYADITQPDQQNNPDHI
                     VLKGVQASSRTDLLNIVSDRLPEYDITGGADNSWNMSMKPSLLTDLKNKAVTQAIETI
                     RNRVDQLGVSEPQIEEHGLGQYQILVQLPDVDDP"
     gene            complement(682..1008)
                     /gene="yajC"
                     /locus_tag="VEJ00_04030"
     CDS             complement(682..1008)
                     /gene="yajC"
                     /locus_tag="VEJ00_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXX00361.1"
                     /translation="MQSLLAIQLGGGGGGMGWLGIAPLIFIFAIFYFLLILPQQRRQK
                     KWQAMLDQLKTGDKVSTSGGLRGTIISMKDDAVQLRIPPDNLRVEVTKASIVHVTTTE
                     EEGKGK"
     gene            complement(1169..2365)
                     /locus_tag="VEJ00_04035"
     CDS             complement(1169..2365)
                     /locus_tag="VEJ00_04035"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXX00362.1"
                     /translation="MSLTFQVEKVSGTARAGRLLTPHGEIETPVFMPVGTVASVKGVP
                     QETLEELGAQIILGNTYHLYLRPGVETVRKLGGLHRFMSWPRAILTDSGGFQVFSLNE
                     LRKLTEEGVTFRSHLDGSSHFFSPESAMEAQIGLGADIIMAFDECTEYPADRERVRAS
                     MALTARWAARSKKYFEEHRNEVPWGGASKTPTLAAKSAARMGHPRAGDSTQSLFGIVQ
                     GGMDRELRRESAERTIEIGFPGYAIGGLSVGEPRELTREIVESTLEYLPADKPRYLMG
                     VGTPEEIVEYAKLGIDMMDCVLPTRAARHGLLFTSEGKISIKQARYAQDQGPLDPNCS
                     CRVCRRYSRAYLRHLYASNEVLAQVLNTIHNLSFYLDTMRGVRHSILLGEESRFLSRV
                     RSRPKP"
     gene            complement(2446..2901)
                     /locus_tag="VEJ00_04040"
     CDS             complement(2446..2901)
                     /locus_tag="VEJ00_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXX00363.1"
                     /translation="MIGVISDTHGLLRPAALGALRGAKHIIHAGDVGTPEILEQLAAI
                     APLTAVRGNVDKGAWARKLPETEAVEIAGISIYVLHDIAQLDLKPKAAGFDVVIYGHS
                     HVPKQETRDRVLYFNPGSAGPRRFRLPVTVGRLVLEQGKVRAEIVAIAD"
     gene            3035..3694
                     /locus_tag="VEJ00_04045"
     CDS             3035..3694
                     /locus_tag="VEJ00_04045"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HXX00364.1"
                     /translation="MNFVLNENEVRVLGALIEKDITTPDYYPLSLNALVNACNQKNNR
                     EPVMTLDENSVRDALASLQEKRLAGPASSADSRVAKYEHRLQEVFNFDRREIAVVCVL
                     LLRGAQTPGELRGRTERMYHFEALDDVVATLDRLAQRDPPLVRVLPRQPGTKESRYMH
                     LFAGESGLASAASGVSGTTVSSGAANVDRVTRLEEEVALLRQELSQVQEQLAAFRRQF
                     E"
BASE COUNT          735 a         1196 c         1127 g          829 t
ORIGIN      
        1 ggatcatcca cgtccggcaa ctgcaccaga atctggtact gccccaaccc gtgctcttca
       61 atctgcggct cgctgacgcc gagctggtcg acgcggttgc gtatggtctc tatggcctgc
      121 gtcactgcct tgtttttcaa gtcggtcagt aacgagggtt tcatcgacat gttccaggaa
      181 ttgtcggcgc ccccggtaat gtcgtattcc ggcagccggt cggagacgat gttcagcagg
      241 tccgtgcgcg aactggcctg cacgcccttc agcacaatgt gatcgggatt gttctgctgg
      301 tccggctggg tgatgtcggc gtagtcaatc ttgcggttgc gcatttgctc ccggagaact
      361 tccatggcgt tctgcgcatc gacgtttacc gcgtcgttga cctgcacctg caaaatcagg
      421 tgagttccgc cgcgcaggtc gagccccagg tggatgtgct cgcccatcga cgcgatcatc
      481 ccctgcgggg agaaactctt gggaatgccg aaaattccga acaggaagat gagcagcgtt
      541 gcaacgatga ttcccgctcg ccagagaaga ggcttgttca taaaaaatca ggggccaggg
      601 gccagggccc agtggccagt tgagagtcgt cgcttgcagc caagtgcaac tgacccctgc
      661 cccctgatcc ctgatccctg atcattttcc tttcccttct tcctcggtcg tcgtcacgtg
      721 cacgatcgag gctttggtta cttcgacgcg gagattatcg ggcgggatgc gcagctggac
      781 cgcgtcgtcc ttcattgaaa tgatcgtgcc ccgcagcccc ccgctggtgg agaccttgtc
      841 tcccgttttg agctggtcca gcatagcctg ccatttcttc tggcggcgct gctggggcag
      901 aatcaaaaga aagtaaaaaa tcgcaaaaat gaagatgagg ggcgcaatgc ccagccaccc
      961 catcccgccc ccgccaccac cgagctgtat tgccagcaac gattgcataa tgtgatgtct
     1021 gagcgtatgt atccgcggag tcgcccgcgc ctacctgcgc cgcgggagcc gtcctgtcac
     1081 cacggagttt ggatcgactt cggccgggtt ctgcggcctc gggactaaat ccggggaggt
     1141 cttcccatcg aggtcttgcc cttaacgtct agggtttcgg tcgagaccgg acacgggaga
     1201 gaaaacggga ctcttcccca agtaaaatag aatgccgtac ccctcgcata gtgtcaaggt
     1261 agaaactcag gttgtgaatc gtgttcagga cctgcgccag cacttcgttc gatgcataaa
     1321 gatgccgtaa ataggccctt gaatagcgcc ggcagacacg gcaggaacag tttgggtcga
     1381 gcggcccctg gtcctgggca tagcgcgcct gcttgatcga gattttgcct tctgacgtga
     1441 acaggagccc gtgccgggcg gcccgcgtag gcaatacgca gtccatcatg tcgatgccca
     1501 gtttggcgta ttcgacaatc tcttcgggcg tgcccacccc catcagatag cggggcttgt
     1561 cggcgggcaa atactcgagc gtggactcga cgatctcgcg cgtcagttcg cgcggctcgc
     1621 ctacgctgag tccgccgatg gcatagcccg ggaatccgat ttcgatcgtg cgctcggccg
     1681 actcccgccg cagttcgcga tccataccac cctgcacgat gccaaagaga gactgcgtag
     1741 agtcaccggc acgagggtgc cccatcctag ccgcgctttt tgcggctagg gtgggggttt
     1801 tcgatgctcc accccacggc acttcgttcc tgtgttcctc aaaatacttc ttgcttcgtg
     1861 ccgcccagcg tgccgtcaac gccatcgacg cacgcacacg ctcgcgatcc gccggatact
     1921 ccgtacactc gtcgaacgcc atgatgatgt cggcgcccag cccgatttgc gcctccatcg
     1981 cactctccgg actgaagaag tgcgaggagc cgtcgagatg cgaacgaaac gtcacccctt
     2041 cctcggtgag cttacgcagc tcgttcaggc tgaatacctg aaatccgccg gagtccgtca
     2101 ggatcgcccg cggccacgac atgaacctgt gcagcccgcc cagctttcgc acggtttcca
     2161 cccctggccg caggtagagg tgatacgtgt tgcccagaat aatttgtgcc cctaattcct
     2221 cgagtgtttc ctgcgggaca cctttcaccg acgcaaccgt gcccacgggc atgaagacgg
     2281 gcgtctcgat ttcgccgtgc ggagtgagca gccgcccggc gcgcgcagtg ccgctgactt
     2341 tttcaacctg aaaggtaaga gacactgcct gggattgtag caacgtggtc tggcttgcat
     2401 tcaggaatga cgcgcgcagg aggccaaatc tgcaatctga aatcgtcaat ctgcaatggc
     2461 aacgatctct gcacgaacct tcccctgctc gagcacgagt ctgccgacgg tcacgggcag
     2521 tctgaatcgc cgcggaccgg cgctgccggg gttgaaatag agcacacggt cgcgcgtctc
     2581 ctgcttgggg acgtggctat gcccatagat caccacgtcg aagccggcag ctttaggttt
     2641 cagatcgagc tgagcaatat cgtgcagaac gtaaatcgag atgccggcga tctcgacggc
     2701 ttccgtttcc ggtagcttgc gggcccaggc gcctttgtcg acgttgcccc ggacagccgt
     2761 cagtggagcg atggccgcga gctgctcaag gatctcgggc gtcccgacgt ccccggcgtg
     2821 aatgatgtgt ttcgcgccgc gaagcgctcc tagcgctgca ggccggagca ggccgtgcgt
     2881 gtcagaaata acgccgatca tagaaggact cccctgatcc acgtttcatc ctcgctctga
     2941 acttgctcgt ccggccacag cggaaagcaa ttctattaaa ctgactgggc tgacaaggcg
     3001 gttcgtcgcc ttttgcctga ttggatctgc agcgatgaat tttgtcctca acgaaaatga
     3061 agtccgcgtt ctgggtgcgt tgatcgagaa agacatcacc acgcccgact actatccgct
     3121 gtcgctcaat gcattggtca acgcctgcaa tcagaagaac aaccgtgagc cggtgatgac
     3181 actggacgag aactcggttc gcgatgctct tgcgagcctg caggagaaac ggctcgccgg
     3241 acccgccagc agcgccgaca gccgcgttgc caagtacgaa caccggctgc aagaagtttt
     3301 taatttcgac cgacgcgaga tcgccgtggt ctgcgtgctt cttttacgcg gggcgcagac
     3361 tcccggagaa ttgcgcggcc gcaccgaacg catgtaccac ttcgaagcgc tcgacgatgt
     3421 cgtggcgacg ctcgaccggc tcgcccagcg cgacccgccg ctggtgcgcg ttctgccgcg
     3481 gcagcccggc acgaaagagt cacgctatat gcacctgttc gccggcgaat ccggtctggc
     3541 atccgccgct tcgggggttt cgggcacaac ggtttcgtcc ggcgccgcga acgttgatcg
     3601 cgtcactcgc ctcgaggaag aagtcgcgct cctgcgacaa gaactctccc aggttcaaga
     3661 acaactcgcc gctttccgca ggcagttcga atgagaggga tattctcttc tgcacggggc
     3721 cgcggggaac atttcccggt cgccgaacgt accattccct agaaggagtt tcagccatgt
     3781 ttactttcct tctgtggtgt gtgctctttg tcctgtgctg gcctctggcg ctgctggcgc
     3841 tgatcgccta tccgttcgtg tggctgttgc tgctgccgtt tcgcctt
//
LOCUS       DASPUL010000166         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_848175, whole genome shotgun sequence.
ACCESSION   DASPUL010000166 DASPUL010000000
VERSION     DASPUL010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848175"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VEJ00_04050"
     CDS             complement(<1..219)
                     /locus_tag="VEJ00_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00365.1"
                     /translation="MRIPLWLKLGWTLWLAIWAPLYWRQYGPQNFLFFCDIGNVLIGI
                     GLWLESSLIFSWAACGLLLFQSLYIVDLV"
     gene            429..2135
                     /locus_tag="VEJ00_04055"
     CDS             429..2135
                     /locus_tag="VEJ00_04055"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXX00366.1"
                     /translation="MGYQALLFCPDEKTARTVTQVLSELEFAVEASVEPFAAVKKLMG
                     QHFDAIVVDCDNEQNATLLFKSARNSASNQAALAVAVVEGQAGVAKAFRIGANLVLTK
                     PINVEQAKGTLRVARGLLRKGEPAKSAAPSPVPVSAPAKGASPATSKPASAPAPRPAQ
                     PVAARPAASAAWPQTASAAAHADSPSEIRGEGATASSSGRMAPASPMPKTGASAESKP
                     AVASAALAHSGGAASAPAPAREVPASHLAAAAPSSAVHPAEAASSAAAAEPVAAPSFA
                     LGGSEASADSGVDRKKIFLGIAAVVLIVAGIVTAWSYFGRRSQSAANPASATPASAAT
                     APASKPGDIAPTAPAPVSTAPASAEQPVSKPTAASTVPRVTNDDEESAGDETGAASTS
                     GPAKSTNSATPAASKAANVAKPEAAPLVVKGGEVPKVQNKAAAAAADAPAPSMIGLAT
                     PGSGGSLPNIVNSGSSAPRPILQTLNISQGVSQGLLMKKVQPVYPRNALSMRVEGTVQ
                     LLATISKSGDISAVKVLSGPAQLTGAAADAVKQWKYKPYLLNGEPVEIQTQVTVVFRL
                     PR"
     gene            complement(2177..>2542)
                     /locus_tag="VEJ00_04060"
     CDS             complement(2177..>2542)
                     /locus_tag="VEJ00_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXX00367.1"
                     /translation="HWDAPVTESPTLDSIEIWELVNTTDDAHPIHLHLVRFQILDRRS
                     FDVGAYWNTGSLKYRAPAVVPDPTEAGWKDTVRADPGMVTRIIARFEGFPGRYVWHCH
                     ILEHEDNEMMRPYDVVAPK"
BASE COUNT          535 a          797 c          772 g          438 t
ORIGIN      
        1 aacgagatcg acgatgtaga gcgactgaaa gagcagtaat ccgcatgcgg cccaggagaa
       61 tatcagcgag ctctccagcc acaggccgat tccgatcaga acgttgccga tatcgcaaaa
      121 gaagaggaaa ttctgtggcc cgtattgccg ccagtagagc ggcgcccaga tcgcgagcca
      181 gagagtccag ccgagcttga gccagagtgg gatgcgcacg cggcgttatt gtatgcgacg
      241 agcccgggat cagtggtccg tgaacagcga tcagcgatca ggggggttat ttaaggaggg
      301 aacgggcccg caaccgatca caatcccctg cgggtctctg tcctgcaaga cacctctaaa
      361 aatcaacgta acccgaatga ccaacgtaac ttgccgctcc tgacgggcgt acctacgatg
      421 gttttagtat gggttaccaa gctcttctct tctgccctga tgaaaaaaca gcccgcacag
      481 tgactcaggt gctgagcgag cttgagttcg cggtcgaggc cagcgtggag ccgtttgcgg
      541 ccgttaagaa gttgatgggc cagcacttcg acgcgatcgt ggtcgattgc gacaacgaac
      601 agaacgctac acttttgttc aagagcgcgc gtaactcagc ctccaaccag gcggcgctgg
      661 cggttgcagt cgtggaaggg caggcgggcg ttgccaaggc gttccgcatc ggcgccaacc
      721 tggtcctgac aaaacccatc aatgtcgaac aggcgaaggg aacgttgcgc gtagcccgcg
      781 gattgttgcg caagggcgag ccggccaaat cggcggcgcc aagtccggtc ccagtttccg
      841 cgcccgcgaa gggtgcgtcg ccggcaacgt cgaagcccgc atcggcgcca gctccgcgcc
      901 cggcccagcc ggtggcggca aggcccgctg caagtgccgc ctggccacaa acagcaagcg
      961 ctgcggctca cgcggacagt ccatccgaaa tccgaggaga aggagctact gcttcgtcgt
     1021 ccggaaggat ggcaccggca tcgcccatgc cgaagaccgg agcttccgcc gagagcaaac
     1081 ccgcagtcgc atcggccgct cttgctcaca gcggcggagc tgcaagcgct cccgcgccgg
     1141 cccgcgaagt accagcttcc cacctcgcgg cagccgcgcc ctccagcgct gtccatccgg
     1201 cggaggccgc ctcaagtgcc gccgcagccg agcctgtagc cgctccctcg ttcgcacttg
     1261 gcggaagcga agcttctgcc gactcgggcg tggatcgcaa aaagattttt cttggcatcg
     1321 cagccgtggt gctgattgtc gccggcattg tgaccgcgtg gtcgtacttt ggacgtcggt
     1381 ctcaatcggc cgccaacccg gcatccgcaa cgcccgcgtc agccgcaact gctccggctt
     1441 caaagccggg cgacatcgcc cctactgctc ccgctcccgt gtcaacggcg cccgcatccg
     1501 ctgagcaacc cgtaagcaag ccgaccgcgg caagtactgt acccagggtg acgaacgatg
     1561 atgaggaatc agcaggcgac gagacaggcg cggccagtac ttcaggtccg gcaaagtcca
     1621 cgaactcggc cacgcccgcc gcgagcaagg cggccaatgt ggccaaacca gaagcggctc
     1681 ccctggtcgt caagggcgga gaagtcccca aggtgcaaaa caaggcagca gccgcagcgg
     1741 cggacgctcc tgctcccagc atgatcggac tcgctacccc gggttcaggc gggtcgctgc
     1801 ccaacatcgt caatagcgga tccagcgcac cccgcccgat cttgcagact ctgaacattt
     1861 cgcagggcgt gtcgcaaggt ttgttaatga agaaagtgca gcccgtctat ccgcgcaatg
     1921 cactgagcat gcgcgtggaa ggcacagtcc agttgttggc aacgatttcc aaatctggtg
     1981 acatcagtgc ggtgaaggtg ctgagcggac cggcgcagtt gaccggcgct gccgcagacg
     2041 ccgtcaagca gtggaagtac aagccgtatc tgctgaacgg cgagccggtc gagatccaga
     2101 cgcaggtcac ggtcgtcttc aggctgccac ggtagaccag accgtcactt catcaacgct
     2161 tcacttcatg gaagagttac ttcggtgcga ccacatcgta gggccgcatc atttcgttgt
     2221 cctcgtgctc caggatgtgg cagtgccaca cgtaacgtcc ggggaatcct tcgaagcgcg
     2281 caataatccg cgtcaccatc ccgggatcgg cacgcactgt gtctttccag ccagcctctg
     2341 tgggatcggg cacgacggct ggcgctctgt atttcaagct tcccgtgttc cagtaagcac
     2401 caacgtcgaa gctcctgcgg tcgaggatct ggaagcgaac caggtgcaga tggatgggat
     2461 gcgcatcgtc ggtggtattg accaattccc agatctcaat cgaatcgagt gtaggactct
     2521 ccgtgacggg tgcatcccaa tg
//
LOCUS       DASPUL010000167         3364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_710847, whole genome shotgun sequence.
ACCESSION   DASPUL010000167 DASPUL010000000
VERSION     DASPUL010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710847"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VEJ00_04065"
     CDS             complement(<1..1347)
                     /locus_tag="VEJ00_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXX00368.1"
                     /translation="MATDTLATPAAINYGGTKVFKNFIDGEWVGSSSGETFENRNPAD
                     TRDLVGVFQKSNKDDVNAAVDAAKRAFAKWRLVPAPRRAEIVFRAAEILIERKEDYAK
                     EMTREMGKVLAETRGDVQEAIDAAYYNAGEGRRLFGPTVPSEMPNKFAMAVRQPIGIC
                     GMITPWNFPMAISSWKLLPAVVCGNTCVIKPAQDTPLSTFNLVRALYDAGLPKGVINV
                     VAGFGSMVGTPLAEHPDVRAVSLTGSSEVGRIIGGIAAKSFKHCSLELGGKNPMIVLD
                     DANLDLAIEGGLWGAFGTTGQRCTATSRMIVQKGVYREFVERLVDRAKKLKVGNGLDE
                     TVQMGPAVNQKQLETDLSYVEIGKNEGAKMVCGGNRLDKGDYQYGWFMEPTVFTDVDP
                     KMRIAQEEIFGPVVGVIPCDDLNDAIEIANGIEYGLSSALYTKDVNKAFSAIRDLYA"
     gene            complement(1543..3135)
                     /locus_tag="VEJ00_04070"
     CDS             complement(1543..3135)
                     /locus_tag="VEJ00_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXX00369.1"
                     /translation="MATASANPSERQPQATNTMALIWAIALAKLLFHIYFNNRYGYFR
                     DEFNYMSCGDHLQWGYVDQPPLIPFLIHISRAVLGDSLRAIRFVPALASSLLVVQTAA
                     IARELGGRRYALILSAICAVISGQYLSNGSLLGTNCLEPNLWMGCAYFAILAIKRSDP
                     RYWLWFGVIAGIGMEEKYSIALFGFGMVVGLLLTEQRRVFLNKWIWLGGLAAFLIFLP
                     NLVWNIHYDWPFVQLMHNIRAEHRDVVLGPVDFLVQQLLLDNPLTAPIWLRGLFALLF
                     FARFKPYRALGWCYLVCYAVFYILHGKMYYLAPVYPMLMAAGSCVIEGAIDGPESAPR
                     RLQWLKPAIAVVLLISGAYVAPIVIPIFSPDHFLGYAKKLPFKLPVMEHSHARAALPQ
                     WYADQFGWQEIVDETAVAWNRLTPEERSSDPTKSCGIFAQDYGQAGAIDFLGRRYGLP
                     RSLSGHQTWFLWGPGPYSGNCMIVLDDSRENLEKLWEHVEFVGTSAPNPYALEQQIDV
                     FICRGKKFDSWPALWPKIKHWR"
BASE COUNT          639 a          919 c         1152 g          654 t
ORIGIN      
        1 ggcgtagaga tcgcgaatcg cgctgaatgc cttgttcacg tctttggtgt agagcgccga
       61 cgacaggccg tactcgatgc cgttggcgat ttcgatggca tcgttgagat cgtcgcaagg
      121 aatgacgccg acgaccgggc cgaagatttc ttcttgcgcg atgcgcatct tcggatcgac
      181 atcggtaaac accgtcggct ccatgaacca gccgtattga tagtcgccct tgtcgaggcg
      241 gttgccgccg caaaccatct tcgcgccttc gttcttgccg atctcgacgt aactcagatc
      301 agtctcaagc tgcttctgat tgacggcggg acccatctgc acggtttcgt cgaggccgtt
      361 gccgactttc agtttcttag cgcgatcgac gaggcgctcg acgaattcgc ggtacacgcc
      421 tttctgcacg atcatgcggc tggtcgcggt gcagcgctgg cctgtagtgc cgaaggcgcc
      481 ccacagtccg ccttcgatgg cgaggtcgag gttggcgtcg tcgaggacga tcatgggatt
      541 cttgccgccc agttcgagcg agcagtgctt gaagctcttc gcggcgatgc cgccgatgat
      601 gcgtccgact tcgctcgatc cggtgaggga gactgcgcgg acgtccgggt gctccgcgag
      661 cggcgtgccc accatggaac cgaatccggc gacgacgttg atgactcctt taggcaggcc
      721 cgcgtcgtag agggcgcgca cgagattgaa ggtggagagc ggcgtatcct gcgcgggctt
      781 gatgacgcag gtgttgccgc agaccaccgc gggcagcagc ttccacgacg agatggccat
      841 cgggaagttc cagggcgtga tcatgccgca gatgccgatg ggctggcgca cggccatggc
      901 aaacttgttg ggcatttccg acgggaccgt ggggccgaag aggcgccggc cttcgccagc
      961 gttgtagtag gcggcgtcga tggcttcctg cacgtcgccg cgggtttcgg cgaggacctt
     1021 gcccatctcg cgggtcattt ccttggcata gtcttccttg cgctcgatga ggatttcggc
     1081 ggcgcggaaa acaatctcag cccggcgggg cgccgggacg agacgccact tggcgaaggc
     1141 gcgcttggcg gcgtcgacgg cggcgttgac gtcgtcttta ttcgatttct ggaagacgcc
     1201 gaccagatcg cgggtgtcgg cgggattgcg gttttcgaag gtctcgccgc tcgaggaccc
     1261 gacccactcg ccgtcgatga aattcttgaa gaccttggtg ccgccgtagt tgatcgcggc
     1321 cggggttgca agggtatcgg ttgccatgat gtctccttaa gattgccgaa tgacgattgc
     1381 cgattgacga ttgcggccca gggaagggcc ccggcagtct cctgaactta ttgatgctac
     1441 aacctgcggg gaaggagcgg caaggcgacc accagggcta aagcccgaag cttgcctttg
     1501 ggggtcttta cgcggcccta aagggccgct cttccacaac gactaccgcc agtgcttgat
     1561 ctttggccac agggcgggcc aggagtcgaa cttcttgccg cggcaaatga atacgtcgat
     1621 ctgctgctcg agcgcgtagg ggttcggggc agaggtgccg acgaattcaa cgtgttccca
     1681 gagcttctcc agattctctc tgctgtcgtc cagcacgatc atgcagttgc ccgaatacgg
     1741 gccgggtccc cagagaaacc acgtctgatg gccgctgagc gagcggggca ggccgtagcg
     1801 gcggccgagg aagtcgatgg cgccggcctg gccgtagtcc tgggcgaaga taccgcagga
     1861 tttggtggga tcggaactgc gctcctcggg cgtgaggcgg ttccaggcga cggcagtttc
     1921 gtcgacgatc tcctgccagc cgaactggtc ggcgtaccac tgcggcaggg cggcgcgggc
     1981 gtgggagtgc tccatcacgg ggagtttgaa cggaagtttt ttcgcgtagc cgaggaagtg
     2041 gtcgggtgaa aagatgggga tgacgatcgg ggcgacgtag gcgccgctga taagcagcac
     2101 gacggcgatc gcgggcttga gccactgcag gcggcggggc gcgctttctg gaccatcgat
     2161 cgcgccttca atcacgcacg agccggcagc catcagcatg ggatagaccg gcgcgaggta
     2221 gtacatcttg ccgtgcagga tgtagaagac ggcatagcag accaggtagc accagcccag
     2281 cgcgcgatac ggcttgaagc gggcgaagaa gagcagtgcg aacaatcccc taagccagat
     2341 gggggcggtc aaagggttat cgaggagaag ctgctgcacc aggaaatcga ccgggccgag
     2401 gacaacgtcg cggtgttcgg cgcgtatgtt gtgcatgagc tggacgaagg gccagtcgta
     2461 gtggatgttc cagaccaggt tgggcaggaa gatcaggaag gcggcgaggc ctcccagcca
     2521 aatccattta tttagaaaaa cgcggcgctg ctcagtcagt agcaggccga cgaccatgcc
     2581 gaagccgaac agagcgatcg agtatttttc ttccatgccg atgccggcaa tcacgccgaa
     2641 ccagagccag tagcgcggat cgcttctctt gatcgccaga atcgcgaagt aggcgcagcc
     2701 catccagaga ttgggctcga ggcagttggt gccgagcagg ctgccgttgg aaagatactg
     2761 gccggagatg acggcgcaga tcgcgctgag gatgagggcg tagcggcggc cgccgagttc
     2821 acgggcgatg gcggcggttt ggacgacgag cagggaagaa gccagcgccg gcacgaagcg
     2881 gatggcgcgg agtgagtcgc cgagcacggc tcggctgatg tggatgagga acgggatgag
     2941 cgggggttgg tcgacgtatc cccattgcag gtggtcgccg catgacatgt agttgaactc
     3001 gtcgcggaag tagccatagc ggttgttgaa gtagatgtgg aagaggagtt tggccagggc
     3061 gatagcccag atcagggcca tggtgttcgt ggcttgcggt tgtcgctcgg aggggttcgc
     3121 ggatgccgtc gccatgggag agaaggatat ccgaatggta ggtacgtagc atccattgcc
     3181 aggttccctt ctttcggccc tggggtctcg gagtcgggcc ggtagcactc cggcaccaag
     3241 gtaacctccc aaagtcattc cctatgacct cagctgtttt gcctgtgcta ccgttttctt
     3301 ttaccgcata ttcgcaaagg atttagcagg atctaagacg agtgtcggac gatcgcaaac
     3361 cggg
//
LOCUS       DASPUL010000168         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1533926, whole genome shotgun sequence.
ACCESSION   DASPUL010000168 DASPUL010000000
VERSION     DASPUL010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533926"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..3532)
                     /locus_tag="VEJ00_04075"
     CDS             complement(281..3532)
                     /locus_tag="VEJ00_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXX00370.1"
                     /translation="MARARYATDSSPVLFLSVIFLSAVFLFAIGVVCPLPLEAQDSAT
                     GIVSGTVLDTSGSRIAKASVVIVNTATGRRYSVNSDEEGRFTIDLLPPGNYSARVVAD
                     GMSPQVTPQLKVDVGGMVQLEFRLSVAGANEKVTVSAAPLLVETKPSAVSTLLDDRAL
                     GDMPLNGRRFSDLALFSPGVTQDPRSLTSSTNGDLAFGGIRGFQNTFLVDGSDYNNAF
                     FAQARGRYRAPYQLSTEVVQEFRVSSNSYGAEQGRSGGAVVNVITKSGSNHVHGTAFY
                     YLRDSSFGAADAFLAFKPHNRQQQAGGTIGGPLVRNRAFFFAGFDQHMFHEPNVVEFL
                     DGSSKVVPQAGSGPYTPGDYEATDQALVFATAAQLTALAGEYPAAQIGNSSYGKLDFN
                     LSPRNQLGLRVNTTRYWGANNVFLDPASPVTYDSISDNGQEMVSTETANLSLMSGISA
                     RWISHLRAQFSRDLQQSSTNTSDPLVKISNVLDGIGRSNLLPRQTREHRLHLAETLSF
                     DGRRSSWKFGGDGLLTWIYDFFPSQQSGEFLFYPVKVNPFTFQPMEAGLELTPLRAYA
                     HEVPHYYLQNFGSATSRPDTNEYAAFAQDTIRVTDRLALSLGVRWDLQTFTTKGLISN
                     PLFPPSGKVPFQPFNFAPRAGFAYSFGSSRPLVVRGGYGIFFVRIPQIYNSVVATENG
                     VTDAQVFLNNTRFWDQQVFPAYPNPLVNCPTMAASCSLPAALAQGVANDPSAVTHEAS
                     AFATNFVTPRVQQSSITLEKEVADRTTVALSLLNVRGEHLIRALDVNLPQPTALTYPI
                     FDSTGSIFQGGYYTVDSFATWQFTRTLDCPFPPCINPLGRPIAQLGAIDEFQSAASSV
                     YNGATLSLNRRVARGTYLRIAYTYARAMDDGQDALVAGQPATVQNSYDPSAERGPSVT
                     DQRDRLVAAFSIDPRPFHREHQVLGRIFNDWKISSVVNYGSGRPVNATVAGDPNQDGN
                     DLNDRLPGYSRNAFTGPDYATADLRLTRTLHFFERYRLNLAGESFNLFNRDNQRVAIT
                     SNGLVASPSTFVQSSVTAGIAPYPGYYQLPKNFMRPNAAYAPRQVQLALKLIY"
BASE COUNT          788 a         1210 c         1022 g          673 t
ORIGIN      
        1 ctgtcagaat gcgaaggcca acggatgagg ctgagaccct gggcatgacg acctccttgg
       61 agactctctg gcgaaccgga tcgggcagag accgcgtacg gaaaccagct atattaaaac
      121 gttttattat ccccacgtca cggctatcgt caagtgcgtt ttttgtacta tgcaccgccc
      181 cacataacac tttcatgggc tgaaagtgtg ttctccgacg ccatccgcgc ggaccccgcc
      241 gcaccaggcc tcaatcctgt cctcacggca ggcctcaacc ctagtaaatc agtttaaggg
      301 caagctgcac ttgccggggc gcgtaggcgg cattcggcct catgaagttc ttgggaagtt
      361 gataataacc tgggtacggt gcaatccccg ccgtcaccga gctctgcaca aacgtgctcg
      421 ggctggccac caggccgttc gacgtaatag cgacccgctg gttgtcacgg ttgaagagat
      481 tgaacgactc cccggccagg ttgagtcgat accgttcgaa gaagtgcaac gtccgtgtca
      541 gccgcaggtc cgccgtcgcg tagtccggac cggtaaacgc attgcggctg tagcccggca
      601 ggcgatcgtt caagtcgttg ccatcctggt tgggatcgcc cgcgaccgtc gcattcaccg
      661 gcctcccgct gccgtagttg acgacgctgg aaatcttcca gtcgttgaag attcgcccca
      721 gaacctggtg ttcgcgatga aacggccgag gatcgatcga gaaagctgcg accaggcggt
      781 cacgctggtc cgtgacgctc ggtccgcgct cggccgacgg atcgtaggaa ttctgcactg
      841 tcgccggctg tcccgctacc agcgcgtcct ggccatcgtc catggctcgg gcataagtgt
      901 aagcaatccg cagatacgtt ccgcgcgcca cccgccggtt cagcgacagc gtcgccccgt
      961 tgtaaacgct cgatgccgca ctttgaaact cgtcaatcgc ccccagctgc gcaatcggcc
     1021 gccccagcgg attgatgcac ggcggaaacg gacagtccag cgtccgcgtg aactgccacg
     1081 tcgcgaacga atcgaccgtg taatacccgc cctgaaaaat cgacccggtc gaatcgaaaa
     1141 tcggataagt caacgccgtc ggctgcggca agttcacatc cagcgcgcgg atcaagtgct
     1201 cgccccgtac gttgagcaac gacagcgcaa ccgtggtccg gtccgcaacc tctttctcca
     1261 acgtaatgct cgactgttgc actcgcggcg tcacaaaatt cgtcgcgaat gcggaagcct
     1321 catgcgtcac cgccgacgga tcattggcca ctccctgggc gagcgccgca ggcagcgagc
     1381 acgaagccgc catggtcgga cagttaacca gaggattcgg atatgcggga aacacctgct
     1441 gatcccaaaa ccgcgtgttg ttcaggaaca cctgcgcgtc cgtgactccg ttttccgtag
     1501 ccaccaccga attgtagatc tgcggaatgc gcacaaagaa aatcccgtat cccccgcgca
     1561 ccaccagcgg ccgcgaactg ccgaaggaat acgcaaaccc cgcccggggc gcgaaattga
     1621 aaggctggaa cggaaccttg cccgacggcg gaaacaacgg gttcgaaatc agccccttcg
     1681 tcgtgaacgt ctgtaaatcc catcgcaccc caagactcaa agccagacgg tccgttactc
     1741 gaattgtgtc ctgcgcgaat gcggcgtatt cattcgtgtc tggtcgcgac gtcgcgctcc
     1801 cgaagttctg cagatagtaa tgcggcacct cgtgcgcata cgcccgcagc ggcgtcagtt
     1861 ccaggccggc ttccatcggc tggaacgtaa atggattcac cttgaccggg taaaacaaaa
     1921 attccccgct ctgctggctg gggaaaaaat cgtagatcca ggtcaaaagc ccgtcgcccc
     1981 cgaatttcca cgagctgcgc cgtccgtcaa agctcaaagt ctcggcaaga tgcaggcgat
     2041 gttcgcgagt ctgcctcggc agcaggttcg accgtcctat gccgtcgaga acattcgaga
     2101 tcttcaccag cggatcgctc gtgttggtcg acgattgctg caagtcgcgc gagaactgtg
     2161 cccgcagatg actgatccag cgcgcggaaa tgccgctcat cagcgaaagg ttcgccgtct
     2221 ctgtcgaaac catttcctgc ccgttatcgc tgatcgagtc gtacgtcacc ggactggcgg
     2281 gatcgagaaa cacattgttc gctccccagt aacgcgtggt attgacccgc aacccgagct
     2341 ggttgcgtgg gctgagattg aagtccagct tgccgtacga tgagttgccg atctgcgctg
     2401 ccggatattc ccccgccagc gctgtcagct gcgcggcggt tgcaaacacg agcgcctgat
     2461 cagtggcctc atagtctccc ggcgtataag gcccgcttcc cgcctgcggc acaaccttgg
     2521 aactgccgtc caggaactcc acgacattcg gctcatgaaa catgtgctga tcgaatcccg
     2581 cgaagaagaa cgctctgttc cgaaccagcg gacccccaat cgtccctccc gcttgttgct
     2641 gccgattgtg cggcttgaag gcaagaaacg catctgccgc tccgaacgag ctgtcgcgca
     2701 agtagtagaa agcagtaccg tgcacgtggt tcgatcccga tttcgtgatc acgttcacta
     2761 ccgctccacc cgaccgcccc tgctcggccc cgtacgaatt cgacgacacg cgaaactctt
     2821 gcacaacttc ggtcgataac tgataaggcg cccgataccg cccccgtgcc tgagcgaaaa
     2881 acgcgttgtt atagtcgctg ccgtcgacca gaaacgtatt ctgaaacccg cgaatcccgc
     2941 cgaacgccag atcgccgttc gtcgacgaag tcaagctccg cggatcttgc gtcacccccg
     3001 gcgagaacag ggccagatcc gaaaagcgcc gcccgttcag cggcatgtcc ccgagcgcgc
     3061 ggtcgtccag cagcgtcgac accgcgctgg gcttggtctc cacaagcaat ggcgccgccg
     3121 acaccgtcac cttctcattc gcacccgcca cgctgagccg gaactccagc tgcaccatgc
     3181 cgcccacgtc cacctttaat tgcggagtca cctgcggtga catcccgtcc gccaccaccc
     3241 gcgccgagta atttcccgga ggcagcaggt cgatcgtgaa gcgcccctcc tcgtcgctgt
     3301 tcacgctgta acgcctgccc gtagccgtgt tcacaatgac caccgaggcc ttcgcaatgc
     3361 ggctcccgct cgtatcgagc acggtgccgc tgacaatccc ggttgcggag tcctgcgctt
     3421 ccagcggaag tggacacacc accccgatcg caaagagaaa gacggcagaa agaaaaatca
     3481 ctgaaagaaa aagaaccgga gaggaatccg tagcgtagcg cgcccgagcc ataccgtcgc
     3541 ccctgcctgc gaaataggct cggaggggac cgcccggcgc acgaaccgga cgcgcggcgg
     3601 gaaagctccg cggagagtgc cggaagccgc atcccaagca tgcggggccg gaatcccctc
     3661 cgcggaatta tgagaaacca gcccctgtat acc
//
LOCUS       DASPUL010000169         3999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1675228, whole genome shotgun sequence.
ACCESSION   DASPUL010000169 DASPUL010000000
VERSION     DASPUL010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675228"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1353)
                     /locus_tag="VEJ00_04080"
     CDS             complement(<1..1353)
                     /locus_tag="VEJ00_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HXX00371.1"
                     /translation="MGQRMKAIDYATVAVFLIGIFWLGSWFYRWVGTPDDFYLADRQL
                     TPFILAASLTTANISLFSLIGVSGTAYQSGISIIWLTWTGNMALVFSGLYVIPLLRRL
                     RIRTIPEFLEIRYGPGVRSLVGVLWIFRLTFWISVVLYAGVIAAQQLTGIRSFVLWIL
                     ALSVIVIVYTTAGGMWSVVLTNNLGFLLVMVSVLTVLPISMRAVGWWPGLAAHLPSEH
                     LEFVLQSGKYNWKFIIAILLLGLEWACLDQGLLQSAFSARDPGVVSKGMVLSAVMITP
                     FAFLWITPGLAAKVLYPGLPKGEMAVPTLIVSLLPAGALGLVTCGFLASGLSTIGSNL
                     GAVATLMTNDIYGRFINRGASPRQLIVAARTATLAAGLLMMAITCLIPYLGGAVDAYL
                     TIISILDMPLFVIAIVYGLLWSKATWQGAMAGYIAGAIAGATLRFGFHFEVALVTLIS
                     G"
     gene            complement(1338..2297)
                     /locus_tag="VEJ00_04085"
     CDS             complement(1338..2297)
                     /locus_tag="VEJ00_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HXX00372.1"
                     /translation="MDIVVVGDINVDLILAGLPSLPAFRELKHAKSMRFTLGGSSAIF
                     AHNIARLGAATGFVGKVGKDHLGDFLIDTLKSAGVDTSRVVRDSALPTGICVSMTFPQ
                     EYAMVSYPGIRESFVLREVDLDYVRRARHLHLSSFYLQPGLQPGCLELFQCAKAAGLT
                     TSLDPDHDPKEKWNSGLQQLLQAVDLFLPNEVEATRITGVQDVDAAAKRLGAYGNTTI
                     IKRGVDGVTVVRGNRVTSAPSFSIEPVDTTGAGDSFNAGFIFQHLRGAPLPQCVVWGA
                     ACGALCTRALGGTEAFPTITEVEEFLATRKPEVAAMKGGWASA"
     gene            complement(2329..3339)
                     /locus_tag="VEJ00_04090"
     CDS             complement(2329..3339)
                     /locus_tag="VEJ00_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00373.1"
                     /translation="MTNYTLSEIRSQPEVWQQIVDLCEGHRHEKVTEWMSSGHPVLTG
                     SGSSFYVCLAAAALFTQLTGRRALAVSASEVCTFPKALFSEAEKYSLLAVSRNGKSVE
                     TVEAARWFKECRSAPAVAVSTVTGSPLLEICEPGLMLTSAAEQSRFMTRSFTGALLGI
                     HYLVASHAGNKELLSELRRLPEICRGVLERCLDSVKFLAEQKHFEDYVGLGQGPFYGL
                     AAESMLKVKEMVKAPAEAYPSLELMHGPNYLLSKTTLVTLLQARSAQKYEFGLLERLR
                     PLGACVFVICEKATAEIRETADFVFEIDSGLSELASLILAMPVMQLFAYYRAQAAGNQ
                     IE"
     gene            complement(3336..3485)
                     /locus_tag="VEJ00_04095"
     CDS             complement(3336..3485)
                     /locus_tag="VEJ00_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00374.1"
                     /translation="MPEKTKLMQIDWITWSVFAIGLILLVYWCVQTIREFADLFRKRF
                     KRTKQ"
     gene            3718..>3999
                     /locus_tag="VEJ00_04100"
     CDS             3718..>3999
                     /locus_tag="VEJ00_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00375.1"
                     /translation="MFNRREWLQGAVILAGVAAQSARGLASSGAAPQKTEPGSLPLSE
                     FAPKSMLQVHESHIERARFPAIDFHTHISTSAKSERGVALAAERKYLGAP"
BASE COUNT          933 a         1236 c         1066 g          764 t
ORIGIN      
        1 tccgctgatc agcgtcacga gcgccacttc gaagtgaaag ccaaaccgca gagtagcccc
       61 ggcaatcgct cccgcaatgt accctgccat cgcaccttgc caggtcgctt tgctccacag
      121 caatccatat acgatggcga ttacaaacaa cggcatgtcc agaatactga tgatggtgag
      181 gtacgcgtcg acggccccgc ctagatacgg aatcaggcag gtgatcgcca tcatcaagag
      241 tcccgccgcg agggttgccg tccgggcggc aacgatcaac tgacgcgggg acgctccccg
      301 attgatgaag cggccataaa tgtcgtttgt catgagagtc gccactgcgc caaggttcga
      361 gccgatcgtc gacagccccg aagcaaggaa gccacatgtc accaacccca gagcgccggc
      421 agggagcaaa ctcacaatca gcgtgggcac tgccatctcg ccttttggta gtccgggata
      481 gagcaccttc gcggccaggc cgggcgtgat ccacagaaaa gcaaaagggg tgatcatgac
      541 tgccgacagc accatgccct tcgaaaccac gcccggatcc cgcgccgaga acgctgactg
      601 caacagaccc tgatccaggc aggcccactc caatcccagc agcagaatcg cgatgatgaa
      661 cttccagttg tattttcccg actgcagcac gaactccaga tgttcgctgg gcagatgagc
      721 ggccagtccc ggccaccacc cgaccgcgcg catcgaaatc gggagcaccg tcagaacgct
      781 caccatcacc agcaggaaac cgagattgtt ggtcaataca accgaccaca ttccgccggc
      841 tgtcgtatac acgatcacga tgacggacag cgccagaatc cacaaaacga acgagcgaat
      901 tcccgtcagc tgctgcgccg cgatcactcc ggcgtacagg accacgctga tccagaacgt
      961 gagccgaaaa atccacagca ctccgaccag gctgcgcacg ccgggtccat agcggatctc
     1021 gagaaactcc gggatggtcc ggatccgcaa acgccgcaga agagggatca cgtagaggcc
     1081 gctgaaaacc agggccatgt tgccggtcca tgtcagccag ataatggaaa ttccgctctg
     1141 atatgcggtc ccgcttacgc caatcagact gaacaggctg atgttcgccg tcgtcaggct
     1201 cgcagcaagg atgaatgggg tcaattgccg gtcggcgagg tagaaatcgt ccggagtgcc
     1261 aacccaccga tagaaccacg aacccagcca gaagatcccg atcagaaaga cagcgacggt
     1321 cgcgtagtcg atcgccttca tgcgctggcc catccgcctt tcatggccgc gacctcgggc
     1381 ttcctcgttg caagaaactc ttcgacctca gtgatggttg gaaatgcctc ggttcctccc
     1441 agcgcacgcg tacaaagtgc gccacaagca gctccccata ccacgcattg cggcagagga
     1501 gctcctctca agtgttggaa gatgaatcca gcattaaagc tgtcgccagc tcccgtggtg
     1561 tcgactggct cgatgctgaa cgatggagca gaagtgacgc ggttgccacg aactaccgtt
     1621 acaccatcca ccccgcgctt gatgatcgtc gtgttcccat aagcgcccag ccgtttcgcc
     1681 gcagcatcga catcttgcac tcccgtgatt ctggttgctt ccacttcgtt cggcagaaac
     1741 agatccaccg cctggagcaa ctgctgcagt ccactgttcc acttttcttt gggatcatga
     1801 tcgggatcga gcgaggtcgt gaggcccgca gctttcgcgc actgaaacag ctcaagacag
     1861 ccaggctgga gtccgggctg caggtagaag ctcgagaggt gaagatggcg cgcccgtcga
     1921 acgtagtcaa gatcgacttc tctgagaaca aagctctccc gaatgccggg atagctgacc
     1981 attgcgtact cttgcggaaa ggtcatcgac acgcaaatcc cagtgggtag tgccgagtct
     2041 cggacaacgc gcgatgtatc tactcctgca cttttcaggg tgtcgatgag aaagtcgccg
     2101 aggtgatcct ttccgacctt gcccacaaat cctgttgccg caccgaggcg tgcgatgttg
     2161 tgagcaaaga tcgccgacga accgcccaga gtgaaccgca tgctcttcgc atgtttcagc
     2221 tctcggaatg ccggcaagga tggcaggccc gccaggatca gatccacatt gatatcgcca
     2281 accaccacaa tatccatgct gctcccgaac ggccgcctcg aatttcacct actcaatctg
     2341 attccccgcc gcttgagccc ggtaataggc gaagagctgc atcacgggca tggccaaaat
     2401 aaggctcgcg agctccgaga gccccgaatc gatctcaaac acgaaatcgg ccgtctcgcg
     2461 aatctcggct gtcgccttct cgcagatgac gaaaacgcaa gctcccaacg gccgcaacct
     2521 ttccaaaagg ccgaattcat acttctgcgc gctgcgggcc tgaagtaaag tcacaagtgt
     2581 cgttttgctt aacaggtagt tgggaccgtg cataagctcc agcgaaggat aagcttccgc
     2641 cggcgctttc accatctcct tcaccttcaa catggactca gccgcgagtc cgtaaaacgg
     2701 cccctggcca aggcccacgt agtcctcaaa atgcttttgc tcggcgagaa acttgaccga
     2761 atccaggcaa cgctccagga ctcccctgca aatttcaggc agcctgcgca actcgctcag
     2821 cagttccttg tttccggcat gggacgcgac gaggtagtga atccccagca gagctcctgt
     2881 aaacgagcgc gtcataaacc ggctctgctc ggcggcagaa gtcagcatta atcctggctc
     2941 gcagatttca agcagagggc tcccggttac cgtggaaact gcaactgccg gagccgatcg
     3001 acactcttta aaccagcgag cggcctcaac cgtctcgacc gatttcccat tccgcgagac
     3061 cgccagaagc gagtattttt ccgcctcaga gaacagggcc ttgggaaacg tgcacacttc
     3121 cgacgcggac acagcgagtg ctctgcgacc agtcaactgc gtgaataagg ctgcggcagc
     3181 caggcacacg tagaaggaac tgccggatcc ggtcagaacg ggatgtccac tgctcatcca
     3241 ttcggttacc ttctcgtgcc tgtgcccttc gcataagtcc acaatttgct gccacacctc
     3301 gggctgcgag cggatctcgc tgagagtgta gttcgtcatt gcttcgtccg tttgaatcgc
     3361 ttccggaaaa gatccgcgaa ctcgcgaatc gtctggacgc accaatacac cagcaggatc
     3421 aggccaatcg caaagacgct ccaggtgatc cagtcgatct gcatcagctt tgtcttttcg
     3481 ggcataagct tgtgatctta agccgtggcc ggaggacagg ccgaaatcaa gtagatgtaa
     3541 atgggccagt ccttcgaatc aagaagcgct taatatgggt cgttcctcgg ggttttcgtc
     3601 ctcgggaagt gaagaaacca gggcaagctg cttgcttttg gagcttacca tgactttctt
     3661 ttactatcga aaacttttct gatatggttt cacaccgaac cagcgacgga gggaactatg
     3721 ttcaaccggc gagagtggtt gcaaggagca gtcatcctgg ccggagtcgc tgcccaatcg
     3781 gctcgaggcc ttgcgtccag cggtgcagcc ccacagaaga ccgagcccgg ttctttgccg
     3841 ctttccgagt tcgcgccgaa gagcatgctg caagtgcacg agtcacacat cgaacgcgct
     3901 cgattccccg ctatcgactt tcacacccac atcagcactt cggccaagtc ggagcgcggc
     3961 gtcgcgctgg cagcggagcg caaatatctc ggagctccg
//
LOCUS       DASPUL010000170         6362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_986447, whole genome shotgun sequence.
ACCESSION   DASPUL010000170 DASPUL010000000
VERSION     DASPUL010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6362
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986447"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2611
                     /locus_tag="VEJ00_04105"
     CDS             <1..2611
                     /locus_tag="VEJ00_04105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXX00376.1"
                     /translation="AGFTALSFRMPEITKRLDRAEIGKRVERAEKLLQKGKTAEALDE
                     YVQVLRDDPDNDIVRQLAADLSLSLNRNGDAVRLLGELFERQVVAGDATRASLTYKKL
                     VRHGSPTWQQKFRFGQLLEGTNKKLAAGTYEAALTDLAKLGKKQETAEILDRLVAIEP
                     TQANLLRVAELASESGDRKKAASAFQRVAQLVSTEGGDAAEWYERAYQEDASDQAIAL
                     AYGKTLLAQGQVGAAIFILEPQLNSGQVTAELRDTYSSALVAAGRYTEAEPLIWALFE
                     QNPSRVQQVIALIGQLLDAEQDEPSVALARKLEQFQRRRGERRQFIAIMQDITATHRA
                     SPPMLEFLSELYNSSNRENDYCQTLLKLFDLYCSTGEFGKAADCLDRAAEVDPYEPGH
                     LKRLDMLKGKVEDGRFKVISSRFNTVSKPAEEPAHTESPTLGASTLQDLMLQAEILVQ
                     YGMRNKAVERLQRIQELFPREEERNEDLQRLYLAAGVTPRYSGSAPLPQSGSAAAPAT
                     AAPAAPAADPAADVRSFTRVAEITRKLYHQSTAPAVLSTAVKEIGSHWEATRCVAAMG
                     KPGLSPTSVDEYCAEGCKKGTASAIAELVSCLQQAVEGQEPLAIQDAPKAAELQSVKK
                     AVTELSAASILAIPLSDGEERVGILVLMHNRSRVWQQADMVVLKTVADQMVIALNNAG
                     LRRLVKNLSVTDEKSGLLKRASYIDLLLAESKRAIQNASALSVLLMQFGRSASMIKEY
                     GETEVQAAMEKVGQMFAANIRSNDLAFRYDTTAIAILLGETAEKEAMLAIEKLRKIIS
                     EVRLSAKDGKEHPVQFSAGLAEAVIRNEYDPVDVVTEVINRAEHALSQAMSQGPGKIV
                     ALGPTALAAGAVA"
     gene            complement(2642..3244)
                     /locus_tag="VEJ00_04110"
     CDS             complement(2642..3244)
                     /locus_tag="VEJ00_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX00377.1"
                     /translation="MKTTGILPLLVLAMTLRLSAAPMSDGDREHLLVHFEMTTQMVAE
                     EVRGLSPAQLEYKASPDRWSIREVVSHLAVAEPDYWRDLMKAVKAPPDMQAKKSSVTD
                     ADIMWYGIDRVVHTKTGGGHEKVETYKDLGEALGKFEALRATMIDYIKTTSDDLRAHS
                     FGEYGQVIDSWQWMLEISTHSERHIQQIREIKADPNFPKK"
     gene            3502..4446
                     /locus_tag="VEJ00_04115"
     CDS             3502..4446
                     /locus_tag="VEJ00_04115"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HXX00378.1"
                     /translation="MPIQKTDKIWHNGKLIAWEDATIHVMSHVIHYGSSVFEGIRCYE
                     VPSGPAIFRAQEHIQRLLDSAKVYRMDVPYTRDQIIKGMLDTVGQNGVWPCYVRPVIL
                     RGYGDAGVNPLNNPIEVYIVNYPWGKYLGHESDACDVCVSSWTRIAPNTLPAMAKSGA
                     NYMNSQLIKMEAIANGYVEGIALDVSGFVSEGSGENLFIVHRDRLITAPLGNSVLPGI
                     TRDAVLQIARDLNLPVIEQMIPREMLYIADEAFFTGTAAEITPIRSVDKVKVGKGTVG
                     PVTKALQKEFYGIVRGEKADRHGWLTPVPVRSKQPVGV"
     gene            4541..5077
                     /locus_tag="VEJ00_04120"
     CDS             4541..5077
                     /locus_tag="VEJ00_04120"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX00379.1"
                     /translation="MSHGLTPEFALGYRAMMLDGINREAQTTRRVIAAIPDDKSDYKP
                     DPHARTAKELAWHLANSDIQFLDGIADMSFSMDTPEHKPETAAEIAAWYDQNIQRGVA
                     RIAAMTPEQLLTPVEFFGVFNFPAVLYLSFLNNHSIHHRGELATYLRPMGSKVPSIYG
                     GSYDEPFVPPAASEETAA"
     gene            5186..6019
                     /locus_tag="VEJ00_04125"
     CDS             5186..6019
                     /locus_tag="VEJ00_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HXX00380.1"
                     /translation="MREPYARYLRLLGFDRAPSGLEGLRQIVRQHVIRVPFENISKLL
                     LIAREHKGRITALPEFLDGLDHYDLGGTCYTANPFFTELLREAGYDADLLGCDMSHRN
                     VHTAIRVRIEGKEYHVDVGYGGPFYEPMPLAELPYEIRHGSFRYVLERAGDGRFAMNV
                     FRDGERVHGYAVNEAPRHLEFFTDIILESFRPTATFMTLLRVVRYFDDHSVELKNSVI
                     TVEHRHNSKRIQLSSLKHLRQTFNEEFRMPRCPVERSVETLEQITGQDFFAMGEGDPF
                     A"
     gene            6147..>6362
                     /locus_tag="VEJ00_04130"
     CDS             6147..>6362
                     /locus_tag="VEJ00_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00381.1"
                     /translation="MNIDDLLRIATERRSSDLHLKVGNYPHLRIDGELVPLTDQPRIS
                     AEDMLNMAFSMMSARQKQKFKETTEIDM"
BASE COUNT         1317 a         2000 c         1849 g         1196 t
ORIGIN      
        1 agcagggttt actgcgctct ctttccgcat gcctgagatc acaaagcgac tggaccgggc
       61 cgaaatcggc aaacgcgtgg agcgcgccga aaagctcctg caaaagggca agactgcaga
      121 ggcgctcgac gaatatgtcc aggtcctgcg cgatgatccg gataacgaca tcgtgcggca
      181 gctggcggcg gacctttcgc tttctttgaa ccgcaacggc gatgccgtcc ggctgctggg
      241 cgaactgttt gagcggcaag tagttgccgg cgatgccacc cgcgccagcc tcacctacaa
      301 gaagctcgtt cgtcatggca gtccgacctg gcaacagaaa ttccgcttcg ggcagcttct
      361 cgaaggaacc aacaagaagc tcgcagcggg aacttacgaa gccgctctga ccgacctggc
      421 gaagctgggg aagaaacagg aaaccgcgga aatcctcgac cgcctggtgg cgatcgaacc
      481 gacgcaggcc aacctgctgc gcgtggccga actggcctcg gaaagcgggg atcgcaagaa
      541 agcagcctct gctttccagc gtgtagcgca gctggtctcc accgagggag gcgatgccgc
      601 ggaatggtat gagcgagcct atcaggagga cgcatccgat caggccatcg cgctggccta
      661 cggcaaaacg ctgcttgccc agggccaggt tggagcagcg atcttcattc tcgaaccgca
      721 gctgaattcc ggccaggtca ccgcggaact gcgcgatacc tactcctcgg cgctggtcgc
      781 agccggtcgc tacaccgagg cagagcctct gatatgggcg ctgtttgagc agaatccgtc
      841 gcgggtgcag caggtgatcg ccctgatcgg ccaattgctc gatgccgagc aggacgagcc
      901 ttccgtcgcc ctggcgcgaa aactggagca attccagcgc cgccgcggcg aacgccgcca
      961 gttcatcgcc atcatgcaag acatcacggc gacccatcgt gcttcgccgc cgatgctcga
     1021 attcctgagc gagctctaca actcgtccaa ccgcgaaaac gactattgcc agaccttgct
     1081 gaagctgttc gatctctatt gcagcaccgg cgagttcggc aaagctgccg attgcctcga
     1141 tcgcgccgcc gaagtcgatc cctacgaacc cggccacctg aaacgcctcg atatgctgaa
     1201 aggcaaagtg gaggacggcc gcttcaaggt catctcctcg cgcttcaata ccgtcagcaa
     1261 gccggcggag gagcccgccc atacggaaag cccgacgctg ggcgcttcca cgctgcaaga
     1321 cctgatgctg caggcggaaa tcctggtgca gtacggcatg cgcaacaagg cagtcgaacg
     1381 cttgcagaga attcaggagc tcttcccgcg ggaagaggag cgcaacgagg atctgcagcg
     1441 gctgtatctc gccgctggcg tcacgccgcg ttattccggt tcggcgcctc tgccgcagtc
     1501 aggctcggcg gcggctcccg caaccgctgc gcctgccgct cctgctgctg atcccgccgc
     1561 agacgtgcgc agctttaccc gcgtcgcgga gatcacgcga aagctttacc accagagcac
     1621 cgctccggcc gtgctgagca cggcggtaaa agaaatcggg agtcattggg aagccacgcg
     1681 ctgcgtagcc gccatgggga agccgggcct gtcgccgacc tcggtcgatg agtattgcgc
     1741 cgagggatgc aagaaaggca ccgcatcggc cattgccgaa ctcgtgtcct gcctgcagca
     1801 ggcggtcgaa ggccaggagc cgctcgccat ccaggacgcc ccgaaagctg cggaactgca
     1861 gtcggtgaag aaagccgtaa ccgaactgtc tgcggcttcc atactcgcca ttccgcttag
     1921 tgacggcgaa gagcgcgtcg ggatcctcgt gctcatgcac aaccgctcgc gggtctggca
     1981 gcaagccgac atggtcgtgc tcaagacggt ggccgaccag atggtgatcg cactcaacaa
     2041 tgccggtctc cgccgcctgg tgaagaatct ctcggttacc gacgaaaagt ccggcctgct
     2101 gaagcgcgca tcctacatcg accttctgct ggccgagagc aagcgcgcca tccagaacgc
     2161 ttccgcgctc tcggtcctgc tgatgcagtt cggccgaagc gcgtccatga tcaaggaata
     2221 cggcgagacg gaggtgcagg ctgcgatgga aaaagtcggg cagatgttcg ccgctaacat
     2281 ccgctccaac gatctggctt ttcgctacga cacgacggct atcgccatcc tgctcgggga
     2341 aaccgccgaa aaagaggcca tgctggcgat tgaaaagctg cgcaagatca tctccgaagt
     2401 gcgcctgtcg gccaaagacg gcaaggagca ccccgtgcaa ttctcggccg gactggccga
     2461 ggccgtaatc cgcaacgagt acgatcctgt cgacgtggtc acggaagtca tcaatcgcgc
     2521 cgaacacgcc ctgagccagg ccatgtcgca aggccccggc aagatcgttg ccctcggccc
     2581 caccgccctc gccgccggcg ccgtggcata gatcccgttt cttcgcgagt tcgataagga
     2641 ctcacttctt gggaaagtta ggatcggcct tgatctcgcg gatctgctga atatgccgct
     2701 cggagtgcgt tgagatctcc agcatccatt gccacgagtc aatcacctgt ccgtattcac
     2761 caaagctgtg agcccgcaag tcgtcgctgg tcgtcttgat gtaatcgatc atggtggcgc
     2821 gcagcgcctc gaacttcccc agggcttcgc cgaggtcttt gtaggtctcg actttctcgt
     2881 gtcccccgcc ggttttcgta tgcaccacac ggtcgatgcc gtaccacatg atgtcggcgt
     2941 cggtcaccga cgattttttg gcttgcatgt ccggaggcgc tttcaccgcc ttcatcaagt
     3001 cgcgccaata gtcgggctcg gcgacggcca gatgggaaac gacctcccgg atggaccagc
     3061 ggtcgggcga ggccttgtac tcgagctgag caggtgaaag cccgcgcact tcctcggcca
     3121 ccatttgtgt cgtcatctca aagtgcacca gcaggtgctc acgatcacca tcgctcatcg
     3181 gagcggcgga gaggcgcaga gtcatcgcca gaacaagcaa gggcaagatt ccggtggttt
     3241 tcatgacggc atcctagcaa ccggccgttc tgaaaaacac gccactgtga ggagcggcgg
     3301 tctatggggg accgtcacac gcatggagtt gacgaaccgg ggcattgccg ccgcgtgcag
     3361 tccaaatcgt ctttggctct ttccaggacg cctctggccc tcggctataa tgacttgtaa
     3421 cgctggtggg gtttgcgcct cgtccctgaa gtgccagagg agacttccgc ctcgttgcat
     3481 accgcgctcg aaggagtctt aatgcccatt cagaagactg acaagatctg gcacaacggc
     3541 aagctcatcg cctgggaaga cgcaaccatt cacgtgatgt cgcatgtgat ccactacggc
     3601 tcctcggtgt tcgaaggcat ccgctgctac gaagtgccct ccgggccggc gattttccgc
     3661 gcccaggaac atattcagcg gctgctcgat tcggccaagg tctaccgcat ggatgtgccc
     3721 tacacgcgcg atcagatcat caagggcatg ctcgacactg taggccagaa cggtgtttgg
     3781 ccctgctacg ttcggccggt cattctgcgc ggctacggcg acgccggcgt caacccgctc
     3841 aacaatccga tcgaggtcta catcgtcaac tatccgtggg ggaaatatct gggccacgag
     3901 agcgatgcct gcgacgtgtg cgtctcctcc tggacgcgca ttgcgcccaa cacgctgccc
     3961 gccatggcaa agtctggcgc gaactacatg aactcgcagc tcatcaagat ggaggccatc
     4021 gccaatggct acgtcgaagg cattgcgctg gatgtgagcg gattcgtcag cgaagggtcg
     4081 ggcgagaacc tgttcatcgt gcatcgcgac cggctgatca cggcaccgct ggggaattcg
     4141 gtgcttcccg gcatcacgcg cgacgcggtg ctgcagatcg cacgcgacct gaacctgcct
     4201 gtaatcgagc agatgattcc ccgcgagatg ctctacatcg ccgacgaggc tttcttcacc
     4261 ggcactgccg ccgagatcac gcccatccgt tccgtcgaca aggtcaaggt aggcaagggc
     4321 acagtgggac cggtcaccaa ggcgctgcaa aaggagttct acggcatcgt gcgcggcgag
     4381 aaggccgacc gtcacggatg gctcacgccc gtcccggtgc gcagcaagca gcccgtcggc
     4441 gtataatcgc ttccgcctcg tttccagaag gggcgcaggc cgcgcccctt ctggcttttc
     4501 ggtcgctgca acacacatca caccctcacc aggaggtcgt atgtcccacg gattgactcc
     4561 cgaattcgct ctcggctatc gcgccatgat gctcgacggc atcaatcgcg aagcgcaaac
     4621 cacccgtcgg gtgatcgccg ccatccccga cgacaagtcc gactacaaac ccgatcccca
     4681 tgcgcgaaca gcaaaagaac tggcctggca cctggccaac agcgacattc agttcctcga
     4741 cggcatcgcc gacatgagct tcagcatgga tacgcccgag cacaagcccg agaccgccgc
     4801 agaaattgcg gcctggtacg atcagaacat ccagcgcggc gtagcccgca tcgccgccat
     4861 gactcccgag cagctgctca cgcccgtgga attcttcggc gtgttcaact tccccgccgt
     4921 gctctatctc agtttcctta acaaccacag catccatcat cgcggcgagc tcgcgaccta
     4981 cttgcgtccc atggggtcga aagtgccctc gatctacggc ggcagctatg acgaaccgtt
     5041 cgtgccaccg gccgcgtcgg aagagacggc cgcttagacg cgttcaatct aaaacctcaa
     5101 agccgcccgg ggtgggcggc ttttcttttc tccactgtct gaccgccaga tttttcttcc
     5161 gcttccttcc catagaatgc gaaagatgcg cgaaccgtac gcacgatacc tgcggctgct
     5221 tggatttgat cgcgctccct ccggcctgga gggcttgcgg caaattgtcc ggcagcatgt
     5281 gatccgggtt ccgtttgaga atatctccaa gctcttgctc atcgcgcgcg agcataaagg
     5341 gcgcatcacc gctctcccgg aattcctcga cggcctcgat cactacgatc tgggtggcac
     5401 gtgctatacc gcaaatccgt ttttcactga actgctgcgg gaagcgggct acgacgccga
     5461 cctgctgggc tgcgacatga gccatcgcaa tgtgcacacc gcgatccggg tacggatcga
     5521 ggggaaggaa tatcacgtcg acgtaggtta tggcgggcca ttctacgagc ccatgcccct
     5581 ggccgagttg ccttatgaga ttcggcacgg gtctttccgc tatgtcctgg aacgagccgg
     5641 ggacgggcga ttcgccatga atgtgttccg agacggagaa cgggttcacg gatacgctgt
     5701 gaacgaagct ccgcgacacc tcgagttttt taccgacatc attctggaat cctttcgccc
     5761 gacagctact ttcatgactc tgctgcgagt ggtccgctac ttcgacgacc actcagtgga
     5821 gttgaagaac agcgtcatta ccgtcgagca ccgccataac agcaagagaa tccaactcag
     5881 ctcgctaaag catctccgcc agactttcaa tgaggaattc cgcatgccgc ggtgtccggt
     5941 ggaaaggtcc gtggagaccc tggagcaaat tactgggcaa gacttcttcg cgatggggga
     6001 aggtgacccg tttgcatagc tgacgccgca cttaagacgc tgtccgcccg ccagttcgag
     6061 gccattacct aggtcatctt ggggaaggtg acgcttcggg cgatcatact gagagcgtca
     6121 ggctaaagca gtccggacct tcttccatga atattgacga cctgctgcgc attgcgaccg
     6181 aacggcgatc ctccgacttg catttgaagg tgggaaacta tcctcacctg cgcatcgacg
     6241 gcgaactggt gccgttgact gaccagcccc gcatcagcgc cgaagatatg ctgaacatgg
     6301 ccttcagcat gatgtcggcg cggcagaagc agaaattcaa ggaaaccacg gaaattgata
     6361 tg
//
LOCUS       DASPUL010000171         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1129022, whole genome shotgun sequence.
ACCESSION   DASPUL010000171 DASPUL010000000
VERSION     DASPUL010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129022"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2303
                     /locus_tag="VEJ00_04135"
     CDS             <1..2303
                     /locus_tag="VEJ00_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX00382.1"
                     /translation="ILQGTSIGGNSRPRVTAPDVRAYTRDTHSFESLGGYSQSVNVEL
                     SGIGEPAQVYASRLTGSVFPTLRVAPLMGRVFTQQDDDQNQQVAVVSYGMWQNRLHGD
                     PHVLGTKILLDRKPYLVIGVMPRDFDFPLLPGRLDHSELWVPMSFEPQELSGAGQANW
                     TFEMVGRLKPGITAEQAQQDAQRVAEETMRAYPPFMANLHIQALVRSLRDETVSQARD
                     LVRILFLATVVVLLIVCANLAGLLLARAIRRRREIAMRMALGAGKIALLRQSVLESLV
                     LSVSGGMLGLVLATIGLQAGRSRLPETLPRVNEIGLDWQVAAFAVAISVLTGIGCGLA
                     PAFAAWHTSVNETLKEGGRTGSAGGHAWLRSALVIGEIAVALVLLAACGLLLRSFEKM
                     RAVDLGFRADHVLSASYALPEKQYPTQNSVNEFNRELVLHAQQLPGVKAAGLTSFLPT
                     SGAWSAGGFVVEGYTPNRGSAMRFSTEVQIEGDFFRAMGISLLAGRFFTVADNADSQL
                     VTIVNRKLAEHYWPDADPIGKRLRIGTPEASTPWLTVVGEVADVNEDSPDIPGKEQLY
                     QPIEQYAKSLGSLASPSILIGNAGFVTLRTETDPEQSSNLLRAAVRSIDPQLPLTQMQ
                     SMERVVSDSEAPRRFNTTLISFFAITAVLLAALGIYSVVAFSAALRVQEMAIRMALGS
                     QRSGILGLVFLSALKLALAGCALGLVAIAAAAHLLDSLLFSVNRFDPLVLAIAAAFVL
                     ALAVAASVLPAARAASIDPMQALRAE"
     gene            2494..2569
                     /locus_tag="VEJ00_04140"
     tRNA            2494..2569
                     /locus_tag="VEJ00_04140"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2527..2529,aa:Thr,seq:tgt)
     gene            2841..2929
                     /locus_tag="VEJ00_04145"
     tRNA            2841..2929
                     /locus_tag="VEJ00_04145"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2875..2877,aa:Tyr,seq:gta)
     gene            2939..3169
                     /locus_tag="VEJ00_04150"
     CDS             2939..3169
                     /locus_tag="VEJ00_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00383.1"
                     /translation="MNRLTAALIAYAVLGVLTWVTISDPRVKVFPLAILALFATKSVL
                     RRHDVMHPDRPEGEASRAEGRKPMADDGFEPM"
     gene            3160..3235
                     /locus_tag="VEJ00_04155"
     tRNA            3160..3235
                     /locus_tag="VEJ00_04155"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3193..3195,aa:Thr,seq:ggt)
BASE COUNT          603 a         1060 c         1093 g          740 t
ORIGIN      
        1 acattttgca gggcaccagc atcgggggta acagccgccc tcgcgttacc gctcccgatg
       61 tccgcgccta cacgcgcgac acccacagct tcgagagcct gggcggatat agtcagtcag
      121 tcaacgtcga actgtcgggc atcggcgagc cagcgcaggt atatgcatcc cgcttgactg
      181 gcagtgtgtt cccaacgttg cgggtggcgc cgctcatggg ccgagtcttc acccaacagg
      241 acgacgatca gaaccagcag gttgcggtag tcagttacgg gatgtggcag aaccgcctgc
      301 acggcgatcc gcatgtgctg ggaacgaaga ttctgctcga tcgcaaacca tatctggtca
      361 tcggcgtcat gccacgtgac ttcgatttcc cgctgctgcc cggacgcctg gatcacagcg
      421 aactctgggt tcccatgagc tttgagccgc aggaattgag cggcgccggg caggcgaact
      481 ggacctttga aatggtgggc cggctcaagc ccggcatcac cgccgaacag gcgcagcaag
      541 acgcgcagcg cgtggccgaa gagaccatgc gcgcgtatcc gccgttcatg gcgaacctgc
      601 acattcaagc cttggtccgc tcgctgcgcg acgaaaccgt ctcgcaggcg cgtgacctgg
      661 tgcgcattct gtttctcgct acggtagttg tgctgctgat tgtttgcgcc aacctcgccg
      721 gattgttgct ggcccgtgcg attcgccggc gccgcgaaat cgccatgcgt atggccttag
      781 gtgctggcaa aattgcactg ctccgccaat ccgtgctcga gagcctggtg ctaagcgtca
      841 gcggaggcat gctcggtctg gtcctcgcca cgataggctt gcaagcgggc cgtagccgcc
      901 ttcccgagac tctgcctcgc gtgaacgaaa tcggcctcga ttggcaggta gccgcattcg
      961 cagtggcgat atctgttctg accggcatcg gctgcggatt ggccccggcc ttcgccgcct
     1021 ggcacaccag cgtcaacgag actttgaaag agggcggccg cactggctcc gccggaggcc
     1081 atgcctggct gcgctccgcg ttggtcatcg gcgaaatcgc cgtcgcgctg gtgttgctgg
     1141 cggcctgcgg cctgctgctg cgcagcttcg agaagatgcg cgccgtcgat ctcggcttcc
     1201 gcgcggacca tgtactttcc gcatcctacg cgctgcccga gaagcaatat cccacgcaga
     1261 actcggtcaa tgaatttaat cgcgagttgg ttcttcacgc gcagcagttg cccggcgtga
     1321 aggctgccgg tttgacttca tttctcccca cttctggcgc ctggagcgca ggcggattcg
     1381 tcgtcgaggg atacaccccg aaccggggct ccgcgatgag gttctctacc gaggtgcaga
     1441 tcgagggcga tttctttcgc gcgatgggca tttcgctgct agcgggtagg ttcttcacag
     1501 tcgcggacaa tgccgacagc cagctcgtca ccatcgttaa ccgcaagctg gccgagcact
     1561 actggcccga tgccgatccc atcggcaaac gcctgcgcat cggtacgccc gaggcttcga
     1621 ctccctggct caccgtcgtc ggtgaagttg ccgacgtgaa tgaggactcg cccgatattc
     1681 ccggcaagga gcagctctat cagccgatcg agcaatacgc gaaatctctg ggctcgctcg
     1741 cctcaccgtc catattgatc ggcaacgcag gtttcgtcac gctgcgcaca gaaaccgatc
     1801 ctgagcagtc gtccaatctc ctgcgcgcgg cggtccgctc catcgatcca caactgccgc
     1861 tcacgcaaat gcaaagcatg gagcgcgtgg tctccgacag cgaagccccg cgccgtttta
     1921 acaccacgct gatctcgttc tttgccatca cggcggttct cctagccgcg ctgggtatct
     1981 atagcgtcgt cgccttctcg gctgcgctcc gggttcagga gatggcgatc cgcatggccc
     2041 tgggctcgca gcgctcgggg atcctcgggc tggtcttcct gtctgcgctc aagctggcgc
     2101 tcgccgggtg cgctctgggg ctggtcgcca tcgccgcggc ggctcacttg ctcgattcct
     2161 tgctgttctc ggtgaaccgt tttgacccgc tggttctggc gatcgctgcc gctttcgtgc
     2221 tcgcgctggc ggtggccgcg tccgtgttgc ccgcggctcg ggcggcgtcg atcgatccga
     2281 tgcaggccct ccgcgcggag taacatgact ttttccccac ctgggctatc ctgcaccctg
     2341 ttccggcggc ttcacgtcgt ccgcaatgac ttgtgacgct aaccctttct tgcacgcagg
     2401 attaggctta tgctacttta tataattgtc ccggtcatct gggcgggatc cgtgggataa
     2461 gtttgcgcgg aggcgcgcgc tgtccccaga cgcgctggcg tagctcagtc ggtagagcat
     2521 ctgatttgta atcagagggt cgggggttcg aatcccttcg ccagctccag agaacgcagg
     2581 aatttagcac gaagacgcaa gttttccggc aggcggcgga ctcggtagcg gcgcagcagg
     2641 attttccaaa cgcactccat tccgaacttt ttccggcttg gtggtcttcg cggcgggttg
     2701 gacgcgaacc ttggtttccc tgtttggcga taagtaaagt gacgtgctcg gcaggttgac
     2761 tgcgagtttt gttgtgcgct ccggcagccg gcagcgatgt ttgctgagcg gatggcttcc
     2821 gcatgggccg agagttttct gcacaggtgg gtgagtggtt aaagccgaca gactgtaaat
     2881 ctgttcctcc tagcgaggta cggaggttcg aatcctcccc tgtgcaccag gaactactgt
     2941 gaaccgcttg accgcagcgc tgatcgccta cgccgtgctg ggagtgctga cctgggtgac
     3001 gatcagcgat ccgcgggtga aggtattccc gctggcgatt ctggcgttgt tcgcgacgaa
     3061 gtcggtctta cgacgtcacg atgtcatgca tcctgacagg cccgaggggg aagccagtag
     3121 agctgagggc cgaaagccga tggccgacga cggttttgag ccgatgtagc tcagttggta
     3181 gagcactccc ttggtaaggg agaggtcacc agttcaatcc tggtcatcgg ctccagaaat
     3241 tgaggcggtt tgcgggcgtg agcgaagcgg gagcccgcag ccgaaatccc gagcgttttt
     3301 cgcgaaggat ctggtccggg aagggcacga cttaagtcgt gccgcctgga gcgaaattga
     3361 ctagcggctc tagccgctga ggttcaagca ggagtaactc agtggtagag tcacagcctt
     3421 ccaagttgag gcgcttgcgg gcgtgagcga agcgggagcc cgcagccgaa atcccgagcg
     3481 ttgtttgcga gggacc
//
LOCUS       DASPUL010000172         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_986656, whole genome shotgun sequence.
ACCESSION   DASPUL010000172 DASPUL010000000
VERSION     DASPUL010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986656"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VEJ00_04160"
     CDS             complement(<1..1430)
                     /locus_tag="VEJ00_04160"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HXX00384.1"
                     /translation="MAALGAAAFVGLLLGPANAQQTDPMDDLLDKLKAKGVLSDDEYQ
                     TLKKAREDEQIELRQERRRQAQQAAQQAEKEQKAADEAAKKPKFDASPGIRSIQLFGD
                     VRLRYESRVATSSFPVGSAAGVLPGAADTETLDRWRYALRIGIRGDLVDDWFYTLRFD
                     TGTNPRSSWVTFGNTGSNNVSTTGGGVAPFGRGSDGIVVGQAYIGWRATPWFTVQAGK
                     MANPLFTTPMVWDPDINPEGIAERFNYAVNDNLNLFANFGQYVYQSFSPNETSGGFGG
                     VDNNGYMFAWQGGVGYKFAPETNARVGLSFYNYAGFPGTVVTGTPSINTNPNGFGGPF
                     AFSTTGAPNTYPTNQAGLNFANGVNNLRYWELPVEVNFPIGGISAQVFGDMSYNIQAD
                     DRAQKGSFASLGSQAFAYQAGMSVGTNIGLTMNRVAAKKNTWEARAYWQQVDLNSLDP
                     NLIDSDFFEGRTNLQGVFLAFAYAPT"
     gene            complement(1811..>2551)
                     /locus_tag="VEJ00_04165"
     CDS             complement(1811..>2551)
                     /locus_tag="VEJ00_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HXX00385.1"
                     /translation="RSGVEIFFTQVFRDGFFHADMHPGNILVDAHGRYIALDFGIMGT
                     LTDTDKSYLARNFLAFFRRDYRRVAQAHLEAGWVPRATRVDEFESAIRAVCEPIFDKP
                     LKEISFGRVLLRLFQTSRRFNVEIQPQLVLLQKTLLNIEGLGRELDPDLDLWKTAKPY
                     LERWMSEQVGWRALTRHMREEAPHWGVALPQLPRLLYRRLLEGDSDAIAAQLAGAAAA
                     QKRQARWLIVLSAVLAVLLVAQLIQLFV"
BASE COUNT          482 a          801 c          786 g          482 t
ORIGIN      
        1 tcggtggggg cgtatgcaaa ggcgagaaag acaccttgca ggttggtgcg cccttcgaag
       61 aagtccgagt cgatgaggtt gggatcgaga gagttaagat cgacttgttg ccagtaagca
      121 cgcgcctccc aagtgttctt cttggccgca acccggttca tcgtcaaccc gatgttggta
      181 cccaccgaca tccccgcctg ataggcaaag gcctgtgagc ccaggcttgc aaagctaccc
      241 ttctgggccc gatcatcggc ttggatgttg tagctcatat cgccaaagac ctgcgcacta
      301 atcccaccga tcgggaagtt gacttcgacc ggcagttccc aatagcgcag attgtttacg
      361 ccgttggcaa aattgagtcc cgcctggtta gtcgggtagg tgttaggcgc accggtcgtg
      421 ctgaaagcga aggggccgcc gaacccattg gggttggtgt tgattgaggg ggtgcctgtc
      481 acgacggtgc ccggaaagcc ggcgtagttg tagaagctca gccccaccct ggcgttggtc
      541 tcgggagcga acttgtaacc gactccaccc tgccaggcga acatgtagcc gttgttgtca
      601 acgccaccaa agccgccgct ggtttcattc gggctgaagc tttgatagac gtactggccg
      661 aagttggcaa acaggttgag attgtcgttc accgcgtagt tgaagcgctc ggcgatgcct
      721 tctgggttga tgtccgggtc ccagaccatc ggcgtggtga acagcgggtt ggccatcttg
      781 cccgcctgca cggtgaacca cggggtggcg cgccagccga tataagcctg gcccacaacg
      841 atcccgtcgc tgcctctacc gaagggcgcc acccctccgc ccgtggtact gacgttgttg
      901 ctgccggtat tgccaaaggt cacccaggac gagcgcgggt tggtgccggt gtcaaagcgc
      961 agggtgtaga accagtcatc cactagatcg ccgcggatgc cgatgcgcag ggcatagcgc
     1021 cagcggtcca aggtctcggt atctgccgct ccaggcaaga cacccgctgc agacccgacg
     1081 gggaaactag acgtcgctac gcggctctcg tagcgcagcc gcacatcgcc gaacagctga
     1141 atgctcctaa tcccaggact tgcgtcgaac ttcggcttct tggccgcctc atcggctgcc
     1201 ttttgctcct tctccgcctg ctgcgccgcc tgctgtgcct ggcgccggcg ctcctgacgc
     1261 agctcgatct gctcatcttc ccgcgctttc ttgagcgtct ggtactcgtc gtcggatagc
     1321 acccccttcg ccttcagctt gtcgagcagg tcgtccatcg gatcggtctg ctgcgcattg
     1381 gccggtccca gcaacagccc gacgaaagct gcggcaccga gcgcggccac gagtctactt
     1441 tggcggttga ccttggatcg gaagatcatt tttttctctc ctgataaacc aaaccgggct
     1501 attcgcttcc gcccttcagc aggcaacaca ccggcgcggc gccaagtcgc gccagctcac
     1561 gccggggcga gcgccgagaa cccgcactcc agcgtcgggg attctgcgga accactatga
     1621 caatttgatg aaacccgcgc ggcctcagat gcctccgcga ggacggcggc accgtcaagt
     1681 cagccagcag cgaatgatcc gggcggccca tggctaaaag cgcgccatgc cctgttgcgc
     1741 ggcgaccttc tcctgagagg atggccgccc gtccgcagcg actggatcgc agggcagcgg
     1801 gtcctgcggg tcagacgaaa agctggatga gctgcgcgac gagcaagacg gcgagaacag
     1861 ccgacaaaac aatcagccac cgcgcctgcc gtttttgcgc agcggccgcg ccggcgagct
     1921 gcgcggcgat cgcgtcggag tcgccttcca gcagcctgcg gtacagcagc cgcgggagtt
     1981 ggggcaatgc cacaccccag tgcggcgctt cctcgcgcat gtgccgggtt aatgcccgcc
     2041 aacccacctg ctccgacatc cagcgctcga gatacggctt ggccgtcttc cataggtcga
     2101 gatcgggatc gagctcacgg cccaggcctt cgatgttgag caaagtcttc tgcagcagca
     2161 ccagctgcgg ctggatttcg acgttgaagc gccgggaggt ctgaaacagg cgcagcagaa
     2221 cgcgaccaaa ggaaatctct ttcaggggtt tgtcgaaaat cggctcgcac acggcccgga
     2281 tggcgctctc gaactcgtcc acgcgcgtgg ccctcgggac ccagcccgct tcgagatgcg
     2341 cctgggccac gcggcgatag tcgcggcgaa aaaacgcgag gaagttgcgt gcgaggtagc
     2401 tcttgtccgt atccgtcagg gtgcccatga tcccgaagtc gagtgcgatg taacgcccgt
     2461 gcgcgtccac gagaatgttg ccgggatgca tgtccgcatg aaagaagccg tcgcggaaca
     2521 cctgcgtgaa gaagatctcg acaccgctgc g
//
LOCUS       DASPUL010000173         7044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_439090, whole genome shotgun sequence.
ACCESSION   DASPUL010000173 DASPUL010000000
VERSION     DASPUL010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439090"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            446..1198
                     /locus_tag="VEJ00_04170"
     CDS             446..1198
                     /locus_tag="VEJ00_04170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXX00386.1"
                     /translation="MRKFDDYMKSYSHLEGWFQLPAIAIWDALLDFQQQQQIVGNILE
                     IGVWKGKSAALAALHCRAEEQCVFVDPLPLDGVRECISAVMPSTKCHYLTESSQFLPR
                     HPFLAQGVRSFRWIHIDGEHTAQAVSNDLAIAELLLGDRGMLVMDDFFNPGYPQVTQA
                     VFQFLNAQPGRLTMVLCGFGKAYLCRPRPAREYLSFMSNSLYSEMNDRQCGGVTIWKT
                     TEPADMNTFGVTERYMDYNYRGPDWNDRQIDI"
     gene            complement(1595..2281)
                     /locus_tag="VEJ00_04175"
     CDS             complement(1595..2281)
                     /locus_tag="VEJ00_04175"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX00387.1"
                     /translation="MGKRTLILAASALSLLGVARAQDDLNSLLPKATAGDPAAQVKVG
                     DIYAIGQGVHRDTTEAAKWFKKAADQKYAAGQYRLGILYELGRGVPREPQTALRFYKL
                     AADQGYADAEYDLAKTYERGQGVPKDDVAAAKWYLSAAQHNKKEAQYMIGSLYEQGRG
                     VKQSYTDALKWYTAAADQSDSEAQYRIGRLYEQGLGVPQSNTRALEWYHKSAEQGNVE
                     AQEALHNLEK"
     gene            complement(2328..5801)
                     /locus_tag="VEJ00_04180"
     CDS             complement(2328..5801)
                     /locus_tag="VEJ00_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00388.1"
                     /translation="MRTDDSVVSRPSGITSGRQTKGRGNTAHGKNSFGLLLAPVVFAL
                     LAVAVLAAPPLAGAQAVTVTVSPGSAQVVTGSSLQLTSTVNGVAPGTQNQLVTWSLSG
                     PGCSGLSCGTINAAGQYTAPASAPNPPTVLATATSIFNPGASGSATLTVVAPAPVIVT
                     VTPSTFSIKPGAQQQFSATVTGTSSNQVVWGASGAGCFGIQCGTIDFNGVYTAPTQVP
                     NPAVVSVTATLLSDQSKSGSAKVTIGSGIAVSVTPPTPQILLGQQQQFAATVTGSNNT
                     VVNWTVSGAGCAGATCGSITAGGLYTAPAAMPGAPTVTITATSVADVNTNGSATLTLF
                     ATPSVSVQPHSVHVNPGAKQVFTATVVGVTDQLVAWSLSGAGCVGIACGTIDPNGNYV
                     APGTIPNPNTVTVTAASLGQPNLSGTATVFVGSGSPVRVAVTPSPANVAAGTSQQFTA
                     KVTGTNNTAVTWSLAGLGCTGSVCGTITSTGLYTAPATLPKFPAVEVIATSQADPTQS
                     GNSFVSILPNIVVTVTPSPVQLKAAQQQQFTATVTGTLSTAVTWSLSGAGCSGAACGT
                     VTSSGLYTAPAQLPNPPVVTVTATSQADSTKSGSATVLLGTPIVVTVTPANVNVTTSG
                     QQKFVAAVTGTKNTAVTWSLTGPACPGSCGSIDSTGLYTAPATVPNSNVTVVATSQAN
                     GVSSGTATVTVISTNVGNFKGQFAFLFRGTDASGFFEAAGSFVADGQGHLLSGVEDIN
                     HPSGPATNVAFTGTYTVSGDNRGTLNWTNAHGTFTYAFALNGKGNLARMIEVDASGRR
                     GSGIMQLQDSTAFNNFGVAGGYAASLVGADSGGTRLGALMSIFPSGTGLVNGSSLDVN
                     DGGQNLATVTFFNGTYSVTGNGRGAMALSIPGFGGGKFNFVIYVISANQFFLISVDPV
                     SGSNPLFSGTALSETGTPFATSSFNGDSIFYETGWTNGAPDVSVGQMTFDGTGIIGVQ
                     YDENAGGQVQIDSGATGTYSVAGNGRSVLNLVNSATHTVSTVTVYAISPNSGFIMDMT
                     ASVRSGYFENQVVKPRFGDVDMLGNYTLGSTGSVGANTPVFTGALTFNANGNMGGNED
                     EDPAGSAELNQLVSGTYAITPVSTTGRGNVFFTLPQSDTVGVWVATFSRAYGIPTTAS
                     DTAPTVLVLEQ"
     gene            complement(5850..6890)
                     /locus_tag="VEJ00_04185"
     CDS             complement(5850..6890)
                     /locus_tag="VEJ00_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXX00389.1"
                     /translation="MKIPAFIVCFLFLTCSFLSAQVTPASITAPSVVADVDGHRLTES
                     DLDRKMSGSLLQARYQYYLTERKALDKLVDDELIALAAERKHMTTDQFLEKEVYKDIK
                     DPSEDQLQVYYEGMESNESYESLHDRLIEHIRDLRRAKARAAYVKTLRTQATLQILLA
                     PPVADVNVEGAELHGGKDAPVVLLEFADYECPYCQKVNPLLVKLREEFGDKITIAYKD
                     FPLPMHRNAQKASEAARCAGEQGKFWEYHDLLYSTKALEVNDLKKHAQTLKLDEAQFN
                     KCLDSGAEADIVKKNLEEGKSLGLSGTPSFFANNHFFSGGVDYNTLREMVEQQIKART
                     TPQSLAQTASTK"
BASE COUNT         1403 a         2079 c         2080 g         1482 t
ORIGIN      
        1 agtgcacgtg cgggtaggtt cgaccggata agatttcatc ggcgatcttc agcgacactt
       61 cattgttgaa gagtcgcatg gccaacttct ggccagcgac gtcgatcgtt ccgacttcta
      121 ttacgtcaga gtcgctcatc agcggttcca gttgcagggg gcttgattaa gacacaatcg
      181 cccgccagaa acctaagcaa ttcggtgccc tcatcttcct ctaatggaaa tcgacgtggc
      241 tcgatcctcg ccgcgtcgaa gagaaacggg tgaggaccaa ccactagaag gcatctctgg
      301 tcacctaagc tcaccataac gagcctcgca acagtcttgc attggacccc agcttgctca
      361 cgggagtggc acggacctgg gaagcggcgc cggcactaat cgtgtactca tgaaaagttc
      421 tgtgcagatg aggtaggagg acactttgcg gaaattcgac gactatatga agtcctactc
      481 acaccttgag ggatggtttc aactcccggc catcgcgatc tgggacgcgt tgcttgattt
      541 ccagcagcag cagcagattg tggggaacat cctggagatc ggggtctgga aagggaaatc
      601 ggccgcactt gctgccctgc actgccgtgc cgaagagcag tgcgtcttcg tcgatccttt
      661 gccgctcgac ggtgtgcggg agtgcatcag cgccgtgatg ccgtcgacca agtgccacta
      721 cctcaccgag tcatcgcaat tcctgccacg gcatccgttt cttgcccagg gcgtacgcag
      781 ctttcgctgg attcacattg acggagagca cacggctcag gccgtcagca atgatttggc
      841 aatcgccgag ctgctgctgg gggatcgcgg catgctggtc atggacgact ttttcaatcc
      901 cggctaccca caagttacgc aggcggtgtt ccagttcctg aacgcccagc ccggtcgatt
      961 gaccatggtg ctctgcggat ttggcaaagc ctatctctgc cggccgaggc cggcgcgcga
     1021 atacctgagt ttcatgagca attcgcttta cagcgagatg aacgatcgcc aatgcggagg
     1081 ggtgacgatc tggaaaacaa cggagccagc cgatatgaat accttcggcg tcacggaacg
     1141 atacatggac tacaactatc gcggcccgga ttggaatgac cgccagatcg acatctgagg
     1201 cgcagcttgc gcttggcgag tcgtctgggc gccagtggcc gggaatgacg gggaaacaag
     1261 aagtgagaag ttcagatcgc gaaagacgcc gccaccgagt caggagaatt acaaccggct
     1321 tacggggcgg atacgtggat gcgccgatac ccggtctgta catctacaga taggacagga
     1381 ttccagcgcc gcgtcgtagt acgcgccgca gtgaccgcac ggcagtccca gaagaccggc
     1441 ccgcggacga accgaacgat ccgagacgtt gggtggaggc tgggaaacgc ctgccagaga
     1501 ttcggtgcct gttacttgtg cagacataag gttgggcaga agtgcacccg ggggagggct
     1561 gatcagacat gcatgcggcc gtcgagcgtg cagcctactt ttcgagattg tgcagagctt
     1621 cctgcgcctc tacatttcct tgttccgccg acttgtggta ccactcgagc gcacgtgtat
     1681 tgctctgcgg cactccgaga ccctgctcgt agagccggcc gatcctgtac tgcgcttcgg
     1741 agtcgctctg gtcggcggcg gcggtgtacc acttcagcgc gtcagtatag ctctgcttca
     1801 cgccccgccc ctgctcatag agcgacccga tcatgtattg cgcttctttc ttgttatgct
     1861 gcgccgccga aagataccac tttgccgccg cgacatcgtc cttagggaca ccttggcccc
     1921 gctcgtaggt ctttgccaag tcgtactccg cgtcagcgta accctggtcg gcagccagtt
     1981 tgtagaaacg caaggcggtc tggggttccc gcggaacgcc gcggcctagt tcgtagagta
     2041 tccccaaccg atactggccg gccgcgtact tctgatcggc agccttcttg aaccacttgg
     2101 ctgcttcagt tgtatcccga tggacaccct gaccgatggc gtagatgtct ccaaccttga
     2161 cctgtgccgc cggatcacct gcggtggctt tcggcagcaa gctattgagg tcatcctggg
     2221 cgcgagcaac cccgagcaga ctcaaggcgc tcgcggccag aatcaacgtc cgtttaccca
     2281 tgcttatctc ccaacctctt cccggtcgga tcctcgaccg acgtacgtta ttgctcgaga
     2341 accagcacgg ttggcgcagt atcgctggca gtggtcggaa tgccgtatgc cctcgagaaa
     2401 gtcgccaccc aaacgccgac cgtgtccgat tgcggcaggg tgaagaacac gttgcctcgg
     2461 ccagtggtag aaaccggcgt aatcgcgtac gtgccgctca ccagctgatt cagctcggca
     2521 ctgcccgccg gatcctcatc ctcgttcccg cccatgttgc cattggcgtt gaaggtcaag
     2581 gcgccggtga aaacgggcgt gttggcaccc accgaaccag ttgatcccag cgtgtagttt
     2641 ccgagcatat ccacatcgcc gaaccgtggc tttacgacct ggttctcgaa atacccgctc
     2701 cggaccgatg ccgtcatatc catgatgaac ccggaattcg gcgagatagc gtagacggta
     2761 accgtggaaa cggtgtgcgt cgccgagttc accaggttga gcaccgagcg tccattgccc
     2821 gcgacgctgt aggttccggt agctccgctg tcaatctgaa cctggcctcc ggcattctcg
     2881 tcatattgca cgccgataat accggtcccg tcaaaagtca tctgaccgac tgagacgtca
     2941 ggcgcaccat tggtccaacc tgtttcgtag aagatcgagt caccgttgaa ggaactggtt
     3001 gcaaacggtg tacccgtctc cgacagcgcc gtgccgctga agagcggatt ggaaccgctc
     3061 accggatcga ccgagatcag gaagaactgg ttggctgaga tcacgtagat cacgaagttg
     3121 aacttcccgc cgccaaagcc cggaatgctg agggccatgg ctccgcggcc gttccccgta
     3181 acgctgtacg tgccattgaa gaatgtgacg gtcgccagat tctgaccgcc atcgttcaca
     3241 tcgagagagc tgccgttcac caagcccgtc ccgctcggga agatggacat cagcgctccc
     3301 aaccgggtac cgccggagtc tgcgcccacc agactcgcgg catatccgcc tgcgacgccg
     3361 aagttgttga acgccgtgga atcctgcaac tgcatgatgc cgctcccgcg tcttccagaa
     3421 gcgtccactt caatcatgcg cgcaaggttg ccctttccat tcaaggcaaa ggcatacgtg
     3481 aacgtgccat gggcattcgt ccagttcagg gttccccggt tgtcgcccga cacggtatag
     3541 gtgcctgtga aggcgacgtt cgtagccgga cccgagggat ggttaatatc ctcgactccg
     3601 gagagcaagt gaccttgtcc gtcggcgaca aaggagccgg ctgcctcgaa gaagccgctg
     3661 gcgtcagtgc cccggaacag gaaggcgaac tgtcccttga agttgccgac gtttgtggag
     3721 atcaccgtga cggtggccgt cccggaggac actccgttcg cctgcgaggt ggccactaca
     3781 gtgacgttcg agttcgggac tgtagcgggc gcggtgtaga gaccggtgct gtcgatgctg
     3841 ccgcaagaac cggggcaggc tggtcccgtc agactccagg tcaccgcggt gttcttcgta
     3901 ccggtcacgg ctgcgacaaa cttctgctgg ccggacgtgg tcacgttgac attcgctggc
     3961 gtcacggtta cgacgatcgg agtccccagc aagaccgttg cacttccgga cttggtggaa
     4021 tcggcctgag acgttgccgt tactgtcacg accggcgggt tcggaagttg ggccggcgcg
     4081 gtgtacaaac ccgagctggt aactgtgccg cacgccgcgc cgctgcagcc ggccccgctc
     4141 aagctccagg tgacagctgt gctcagggtt cctgtaaccg tcgcggtgaa ttgctgctgc
     4201 tgcgccgctt tgagctggac tggactcggc gtcaccgtca cgacgatgtt cggcagaatg
     4261 ctgacgaatg aatttccaga ctgcgtcggg tccgcctgtg aggttgctat cacttccact
     4321 gccgggaact tgggcagagt cgccggggcc gtgtacaggc ctgtcgacgt gatcgttcca
     4381 caaacgctgc cggtgcatcc taagccggcc agactccagg taaccgctgt gttgttcgtc
     4441 cccgttacct tggcagtaaa ctgctggctc gttccagcgg ctacattggc tggagacgga
     4501 gtcacagcaa cgcgcactgg agatcccgag cccacaaaca cggtggcagt tccagacaga
     4561 ttcggttggc ccagagatgc ggcggtcacg gtcaccgtgt tgggattggg aatcgtccct
     4621 ggcgccacgt aattaccgtt aggatcgatt gtgccgcagg caatacccac acatcctgct
     4681 cccgacaggc tccaggcgac taactgatcg gtcactccga ccactgtagc tgtaaagact
     4741 tgtttcgctc cagggttcac atggaccgag tgcggttgaa cgctgactga aggggtagcg
     4801 aacagcgtga gtgtagcgct gccatttgtg ttgacgtcgg caaccgaagt tgcagtaatg
     4861 gtcacggtcg gcgctccggg catcgccgca ggtgccgtgt agagacctcc cgccgttatg
     4921 cttccacaag ttgcgcccgc gcaacccgca cccgaaaccg tccagttgac gaccgtgttg
     4981 ttgctcccgg ttacggtcgc ggcaaactgc tgctgttgcc ccaataaaat ctgaggagtg
     5041 ggaggcgtca ccgatacggc gatgccgctt ccgatagtga cttttgctga acccgacttc
     5101 gactgatcag agagcaaggt tgcggttaca gacaccactg cggggtttgg cacttgagtc
     5161 ggcgcagtgt agacgccgtt aaagtcgatg gttccgcact gtataccaaa gcaccccgcg
     5221 ccgctcgcac cccagactac ctggttgctg ctcgtaccgg tcacggtagc gctgaattgc
     5281 tgctgcgctc caggcttgat ggaaaacgtc gacggcgtta cggtgacgat aaccggagcg
     5341 ggtgcgacga ctgtaagagt agcgctgccg ctcgcgccgg gattgaagat cgaggttgca
     5401 gtcgccaaca cggtgggcgg attcggagcg ctggcgggcg cggtgtactg tccggcggca
     5461 ttgatcgtgc cgcaactcaa tccgctgcag cccggacctg agagcgacca ggtgacaagc
     5521 tggttttgtg tgccgggcgc aacaccgttc actgtgctgg taagctgaag cgaactcccg
     5581 gtgacgacct gcgccgaacc cgggctgacg gtgacggtga ccgcctgggc gccggcgagt
     5641 ggcggtgctg ccagaaccgc cactgccagg agagcgaaaa ccacgggtgc caggagcagc
     5701 ccgaaggaat tcttgccgtg ggcggtgttt cccctgccct tcgtctgcct tccagacgtg
     5761 atgccagaag gtctgcttac gactgaatca tcggttcgca tagctgccct gatctttcgc
     5821 cgctcgctct gtacctcgcc gactgcaaac tacttggtgg acgccgtttg ggccagagac
     5881 tgcggggttg ttcttgcctt aatctgttgc tccaccatct cgcgcaacgt gttgtagtca
     5941 actccgccac tgaaaaagtg attgttggcg aagaaactgg gggtacctga aaggccgaga
     6001 ctcttgcctt cttccaggtt ctttttgaca atgtctgctt ccgcgccgct gtcgaggcac
     6061 ttgttgaact gtgcctcatc gagcttaaga gtctgcgcgt gcttcttcag gtcattgact
     6121 tccagcgcct tggttgaata aagcaggtcg tgatactccc aaaacttgcc ttgttcaccg
     6181 gcgcaacgag ccgcttctga tgctttctgg gcgttgcgat gcatgggcag agggaagtcc
     6241 ttgtaggcga tcgttatctt gtcgccgaac tcttctctga gcttcaccag cagcggattc
     6301 accttctggc aatacgggca ctcataatca gcaaactcca acaggactac cggagcgtcc
     6361 ttgcctccat gaagctccgc gccttccaca ttcacatccg ccacaggcgg cgccagcagg
     6421 atctgcaggg ttgcctgggt gcgcagcgtc ttgacgtagg cagcgcgtgc cttcgcacgc
     6481 cgcaggtcac ggatgtgctc gatgagccgg tcgtgcagcg actcgtacga ttcgttgctc
     6541 tccattccct cgtaatagac ctgcaattgg tcttcactcg gatccttgat gtccttgtag
     6601 acttcttttt ccaggaactg atcggtggtc atgtgcttgc gctcggcggc cagagcgatt
     6661 aactcgtcgt cgacgagctt gtccagagcc ttgcgttcag tcaggtagta ctggtaacgg
     6721 gcttgcaaga gactgcccga catcttgcgg tcaagatcgg actccgtaag ccggtggcca
     6781 tctacgtcgg caacgacgga aggagcggta atggaagctg gagtaacttg agcagaaagg
     6841 aacgaacagg tcagaaacag gaaacacaca ataaacgcag gaatcttcac aaatcctccc
     6901 ccaaggaatt gtttcagaac gattaattta gaagtgtgcc aggcgatcct tacgaatcgc
     6961 ctggcacgac tgtgttacgc cgcccagagg gcgggtgagt cgaggttagt caccagtccc
     7021 gcagctgcag atgccgtgcc cgct
//
LOCUS       DASPUL010000174         4071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1808208, whole genome shotgun sequence.
ACCESSION   DASPUL010000174 DASPUL010000000
VERSION     DASPUL010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808208"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /gene="hflX"
                     /locus_tag="VEJ00_04190"
     CDS             complement(<1..961)
                     /gene="hflX"
                     /locus_tag="VEJ00_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXX00390.1"
                     /translation="MGSSHSKKSREGERTGRGAPFGHDPGREEPSGERAFLVGLEVRS
                     RAARGAVTAQAAAARDAAQNASTQIPTPSASSGQALSQKARKDGAPDASRVNGKPSIP
                     EFDADESLAELRTLAESAGAGVVGQILQRRDRLDPATLIGAGKLEEIAGAAASVNADI
                     VLFDHDLSPSQQRNIEKTVHRRVIDRTQLILDIFARHARTREGQLQVELAQLQYLMPR
                     LAGRGVEMSQLGGGIGTRGPGETQLETDRRKIARRMRHVEQQLENVRRIRAQQRQRRE
                     SAPVATVALVGYTNAGKSTLFNALTRAGVLSSPKMFATLDPTIR"
     gene            complement(1108..1302)
                     /gene="hfq"
                     /locus_tag="VEJ00_04195"
                     /pseudo
     CDS             complement(1108..1302)
                     /gene="hfq"
                     /locus_tag="VEJ00_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003211269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
     gene            1709..2446
                     /locus_tag="VEJ00_04200"
     CDS             1709..2446
                     /locus_tag="VEJ00_04200"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HXX00391.1"
                     /translation="MHHFVDLASLVMPTISGKEALQLILRWFHFLAGITWIGLLYFFN
                     LINVPFMKQVDAAMKPKIFQNMTLPALNWFRWSALLTVFIGFWYWAQFYVGADADRQN
                     TSGLSTIGLFLLLWIGSWFILFLVIKKMTPSGYVLGVITAILTYAAGWLFVNRTPVGG
                     DDIHVLAIGVGGGLGFIMLFNVWGIIWPNNKKIIKGTLAGTPPADAAALARQAFLASR
                     TNFFLSVPLLFYMAISSHFASTVIFGK"
     gene            complement(2534..>4071)
                     /locus_tag="VEJ00_04205"
     CDS             complement(2534..>4071)
                     /locus_tag="VEJ00_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00392.1"
                     /translation="NGKTVVRGGFGLYYQQISNQAELQGSLGAPFFLSQITTIGKPAP
                     LQLANPLPNQVSGGGKVLPQYVPQKSIFTGMAPGTGCTGLLGQALLNCEVNDPTATAI
                     WTDPAGTVCGVSGGPALDCGIDLDSFTSADPNLHAPYTEQWNLTVQRDLGHNWALEVG
                     YVGSHNVGGIAIWVPTQALLASPTNTITVQDSSANSYAMTATTFANETLREQALGLSF
                     LTGSSYTSNIGNQIYHSFQTTLSHRFQHGLFFQTGYTWAKNIDNVSGSVKTDELNGTN
                     SSSQGGASIYNDKENISGNRALSDLDRRHRLTMSYGYEFPISKSGILGTQLFQGWGVS
                     GLTTFQSGQVFSVSDSFGGGAYGFGLSTPTAVCGNLVPTLPTCTLGAATDPRQAVTQG
                     STESRLNNWVNPNFFSNPGPANAFSDGISTGWGSPGMRNIYRGPFQQDWDFALLKKFH
                     IMERHQILFRADFFNIFNHPVFNIPTIATGTDINSVPGNVGKITTTVLPARIIQLGLK
                     YSF"
BASE COUNT          740 a         1320 c         1130 g          881 t
ORIGIN      
        1 tgcgaatcgt cggatcaagc gtggcaaaca tcttcggtga cgacaaaact cccgctcgcg
       61 tcagcgcgtt gaacagcgtc gacttccccg cgttcgtata tcccaccagg gccacggtcg
      121 ctaccggcgc cgactcccgc cgctgcctct gctgagcgcg gatgcgccgc acgttctcca
      181 gttgctgctc cacatgccgc atccgccggg caatcttgcg ccggtcagtc tcgagctgcg
      241 tttcgcccgg cccgcgcgtc ccgatgccgc cgcccagctg cgacatctcc acgccacgcc
      301 ccgccagccg cggcatcaga tattgcaact gcgccagctc cacctgcagc tggccctctc
      361 gcgtccgcgc gtgccgtgca aaaatgtcga ggatgagctg cgtgcggtcg atcacccgcc
      421 ggtgaaccgt tttctcaatg ttgcgctgct gcgaggggct gagatcgtga tcgaagagaa
      481 caatatccgc attcaccgaa gccgccgcgc ctgcaatttc ctcgagcttt cccgcgccga
      541 tcaaggttgc gggatcgagt cgatcgcgcc gctgcaggat ctgtccgacc actccggccc
      601 cggcgctctc ggccagcgtg cgcagttcag ccaacgattc gtcggcatca aactccggaa
      661 tacttggttt tccgttgacc ctggacgcat cgggtgcccc atcctttcgc gctttttgcg
      721 aaagagcctg ccctgagctt gccgaagggg tggggatttg tgttgacgca ttctgggccg
      781 cgtcccgcgc agccgctgcc tgcgccgtga ccgcacctcg ggccgcgcga ctgcgcacct
      841 ccagcccgac caggaacgcg cgctctccgc tcggctcttc gcgcccgggg tcgtgcccga
      901 acggcgcacc tctccccgtg cgctcccctt cacgggattt cttggagtga gaacttccca
      961 aacccaggcg ccccgtctct agccttcgct gcccgaagcg ctcgccgcat tcgccgccga
     1021 tgtggccgcc gtcccgccgc ccgccggcgc cttctccgca tgcgccacgt gcatggcgtg
     1081 gggtgtcgcc acccgcccca tcaccaccgt cgagatggcg tgcttgaaga tgagttgctc
     1141 ctgcgagttc gcctccagca ccacggagta cttgtcgaag gagcggattc ggcccgtcag
     1201 ttttaccccg ctcagcaaat aaatcgtgat gcttagtctc tctttgcggg ccgtgttgag
     1261 gaaggaatcc tgtatgtttt gcgccggctt attgctgtcc atcgccgatc tccttgccgc
     1321 tctagactcc cacaagccac ttgccagccc accggccagt catgggcaaa atctgcggca
     1381 tgctcccgaa acttccagca caccacgaaa ggacaatcct gcctcaccga tctactccca
     1441 acgagcgatt taagcaggca gatgtaagta ccatacggcg atcttcgccg cttttcaaca
     1501 cggaagttgc ccgggcttcc aatcttgcgg ttgacattgc ccgcagctaa agtgcagact
     1561 ttcctcgact ccgcgagcct ccggtttcga cgcggccgct cgctggtctc tctcattgca
     1621 ttctggcttt ttcttcgccg agtgcccctt cgtctccggg cattcggggg ccccacaacc
     1681 tcacatcgca gttggcggag gaaatccaat gcatcatttc gtcgatctgg catcgctcgt
     1741 catgcccacc atcagcggca aagaagccct gcagctgatc ctgcgctggt tccatttcct
     1801 cgccggaatc acctggatcg gcctcctcta cttcttcaac ctgatcaacg tccccttcat
     1861 gaagcaggtg gacgccgcca tgaagcccaa gatcttccag aacatgaccc tgcccgccct
     1921 taactggttc cgttggagcg ccctgcttac ggtcttcatc ggattctggt attgggcgca
     1981 gttctacgtc ggcgccgacg ccgatcgcca gaacaccagc ggcctcagca ccatcggcct
     2041 gttcctgctg ctctggatcg gctcctggtt catcctcttc ctggtgatca agaaaatgac
     2101 ccccagcggc tacgtcctgg gcgtgattac cgccatcctg acctacgccg ccggatggct
     2161 gttcgtgaac cgcaccccgg tcggcggtga cgatatccac gtcctcgcca tcggcgtcgg
     2221 cggagggctc ggtttcatca tgctgttcaa tgtctggggc atcatctggc ccaacaacaa
     2281 gaagatcatc aaggggacgc tcgccggtac gcccccggcc gatgccgccg ccctggcccg
     2341 gcaggccttc ctcgcttctc ggaccaattt cttcctgtcg gtgccgctgc tgttctatat
     2401 ggccatcagc tcccacttcg ccagcacggt catcttcggg aagtagccgg cccacgattc
     2461 tttctccttg tgcttgcagg aacgaaaaac gcccgcgctt aagagcgcgg gcgttgtagt
     2521 tttggcagtt cgctcagaag gagtacttca atcccagctg aataatgcga gctggaagca
     2581 ccgtcgtcgt gatcttgcca acgttgcccg ggaccgagtt tatgtccgtg ccggttgcta
     2641 tggtcggaat attgaacacc gggtggttga agatgttaaa gaagtcggcc cggaacagga
     2701 tctggtgcct ttccataatg tggaacttct tcaagagtgc gaagtcccag tcttgctgaa
     2761 aaggcccacg gtagatgttg cgcatccccg gacttcccca gcccgtcgag atgccgtccg
     2821 agaaagcatt tgccggaccg ggattgctga agaagttcgg gtttacccag ttattcaagc
     2881 gactctcagt tgacccctgc gttaccgcct gtcgcggatc agtggctgcc ccaagagtgc
     2941 aggtcggcag agtcggcacg agatttccgc agacggcggt cggcgtactt agtccaaaac
     3001 catacgcccc cccaccgaag ctgtctgaga ctgaaaacac ctggccgctt tggaaggtcg
     3061 tcaatccgct gacgccccaa ccttggaata gctgcgtgcc gagtattccg ctcttggaaa
     3121 tcgggaattc gtaaccgtaa ctcatggtca agcgatgccg gcgatccaaa tcgctgaggg
     3181 cacgattgcc tgagatgttc tctttgtcgt tgtagatact ggcaccgcct tgcgagctag
     3241 agttcgtgcc gttcagttcg tccgtcttca ccgaaccgct gacgttgtca atgtttttcg
     3301 cccaggtata acccgtctgg aagaacagac cgtgctggaa gcgatgcgac agggtagtct
     3361 ggaacgaatg gtagatctgg tttccaatgt tcgaggtgta gctggaaccg gtgaggaatg
     3421 acagccccag cgcctgctca cgcagggtct cgttggcgaa ggtagttgcg gtcatcgcat
     3481 aagaattcgc ggaactgtcc tggaccgtaa tggtgttggt aggactcgcc agcaaggcct
     3541 gggttgggac ccagatggcg ataccaccca cgttgtgcga accgacataa ccgacttcca
     3601 aagcccaatt gtgtcccaag tccctctgca ccgtaaggtt ccactgctcc gtataaggcg
     3661 cgtgcaggtt tggatctgcg gacgtgaagg aatctaggtc gatgccgcag tcgagagcag
     3721 gcccgccaga aacaccgcag acagtacccg ccggatcggt ccatatcgcc gttgctgtgg
     3781 gatcgttcac ctcgcagttc agcagcgctt gaccgaggag tccagtacaa ccggttcctg
     3841 gtgccatgcc cgtgaagatc gacttctgcg gtacgtactg gggcagtact ttgcctccgc
     3901 ccgacacctg attgggcagc gggttggcca actgcaaagg cgctggcttc ccgatggtag
     3961 taatttggga caggaagaac ggtgccccca gcgagccctg tagttcggcc tggttggaaa
     4021 tctgttggta gtagaggccg aaaccgccgc gcacgaccgt tttcccattt c
//
LOCUS       DASPUL010000175         4451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_987831, whole genome shotgun sequence.
ACCESSION   DASPUL010000175 DASPUL010000000
VERSION     DASPUL010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987831"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VEJ00_04210"
     CDS             complement(<1..410)
                     /locus_tag="VEJ00_04210"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXX00393.1"
                     /translation="MRNTILVTGGAGFIGSNFILQRMRDDTSAIVNLDKLTYAGNLRN
                     LESIANDARYEFVQCDICDRALVRRVLEKHLPACIVHFAAESHVDRSIRGPDDFIRTN
                     IDGTFALLEETRAYWSALPDREREQFRFLHVSTD"
     gene            complement(571..3093)
                     /locus_tag="VEJ00_04215"
     CDS             complement(571..3093)
                     /locus_tag="VEJ00_04215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXX00394.1"
                     /translation="MLYCNKYNYRFSGTEVYLFELMNLMRSQGHEVALFSMSDPRGEP
                     TPYDKHLVPHIDFKADAGPWQKARLAAHAIYSRDARRRIRALIRDFRPDVAHVRNIYH
                     HLSPSILWELKAQQVPVVYHVNDFKLLCPSYNLVSCGEPCEACKGGAFWHALRPECYP
                     GIGARITLSAEAYIHKLLGTYRKCVDLFLAPSQFVRDKFVEHGWDGGKFEILPHFQET
                     SSPPACQLASRDDRRPVLYFGRLSAEKGVDDLLRAMQNAPQMRLIIAGDGPQREPLQG
                     LASALGLTNVEFVGHVGPSRRDSLIAESQFTVLPSHAYETLGKTILESYAAGRAVVAS
                     DLGSRRELVREGETGLLYRTGDIGQLACQIEMLGSNPDLAQKMGRAGFDFVRLHHTPE
                     GHYRKLLSFYESVITAKACLVPGTQSQPVTPRRRIFALRRPRPLPVPGLRLRLAFIGG
                     RGLVSKYSGIESYYEEVGKRLVEMGHEVTVYCRNYFTPPIAQRNGMRVVRLPTVRSKH
                     LETLVHTLLSTAHAFTQRYDVVHYHALGPALFSFLPRLVGTRTAVTVQGLDWQRKKWG
                     RLASAVLRAGERASVKFPSGTMVVSRALQHRYREAHGVETFHVPNGGVLREWREARHI
                     LEWGLEPGNYVLFLGRFSPEKNCHLQVNAFEQIQAQLPNNVKLVLAGASSYCDDYSRR
                     LRTHASGRIRMLDWVSGETLDELLTNAMIFVLPSDLEGLSLALLDAMGAGLCVLASDV
                     PENREVVDGAGYTFHRGDVADLADRLCFLIANPAVREATGREARRRIEEQYQWPKIAA
                     DIEEAYFAVMGWSPADGLARKPSARAAAVAAGQSENAERRAG"
     gene            complement(2978..>4451)
                     /locus_tag="VEJ00_04220"
     CDS             complement(2978..>4451)
                     /locus_tag="VEJ00_04220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXX00395.1"
                     /translation="HACRDAGGAAAAGGVSSAMLTVAYLANRFPSPVEPYVVEEIGEL
                     RKRGVRVIAGSVREPATEEVLVAGNSPDITLFPLRAVVLLQALWLCLRRWPRVSDLLW
                     RVLCRGRESPVQRMKALLHTWLGACYAILLREHRVDHIHVHHGYFGSWIAMVAARLLD
                     IGFSMTLHGSDLLLHGSYLDVKLELCSFSLTVSEYNRRFILEQYPEVEPQKVVVSRLG
                     VEVEDHLNFLPRAAERPRAVLNLLSVGRLHAVKDHAFLIRACAQLHATDVQFECAIAG
                     EGPERKKLETLIRRYGLEDRVTLLGHVPREQAASLYDRADVVVLTSRSEGIPLVLMEA
                     MARGKIVLAPAITGVPELVIPGQTGFLYQPGSMGDFVARLLLIHSLMHASGRRAEGHG
                     GGLSANQVSLESVVGDQFDWMRHAARVQIRHNFNRSKNLRSFANTFLQRIFPQTGSLL
                     HENPVLQQVQLPLQRHRSLPVRADEPDAVAGPRSGAVFDV"
BASE COUNT          853 a         1360 c         1447 g          791 t
ORIGIN      
        1 tcgtcggtcg agacgtgcag gaagcggaac tgctcgcgct cccggtccgg cagtgcactc
       61 cagtaagccc gggtttcctc cagcaaggcg aatgtgccgt cgatgttggt gcggatgaaa
      121 tcgtccgggc cgcggataga acggtccacg tggctctccg ccgcgaaatg cacgatgcaa
      181 gcgggcaggt gcttttcgag aacgcggcga accagggcgc ggtcacaaat gtcgcattgt
      241 acgaactcgt agcgcgcgtc gttggcgatg ctctccaggt tgcgcaggtt tccggcgtag
      301 gtcagcttgt cgagattgac gatggcggac gtgtcgtctc tcatccgctg caggatgaag
      361 ttggaaccga tgaaaccagc gccgccggta acgagaatcg tatttcgcat ctgggatgcc
      421 gcgagcccgg agccgtggag gcgccgctgc gacaagtcct tgattgtaat aaaaaaggcg
      481 ggatcagaac ccgccttggc gcaagtatac agatttcctc agattgtggc gctgttaggc
      541 gccacccaca agggctgacg gcgcactcgt ctaacctgcc cgccgttccg cgttttcaga
      601 ttggccggca gcgacggccg cggcgcgggc gctcggcttt ctggccagtc cgtcggcagg
      661 cgaccatccc atcacggcaa agtaggcctc ttcgatgtcg gcggcaatct tgggccactg
      721 atattgctct tcgatccttc ttctcgcttc gcgccccgtg gcttcgcgca cggcgggatt
      781 ggcaatcagg aaacacaggc gatcggccag gtcggcgaca tcgccgcgat ggaacgtgta
      841 cccggcgccg tcgacgactt cacgattctc cggaacatca ctggcgagga cgcaaagacc
      901 tgcgcccatc gcgtcgagca gcgccagcga caggccttcg agatcggagg gcagaacgaa
      961 gatcatggcg ttggtcagca gttcatcgag cgtctcgcca ctgacccagt cgagcatgcg
     1021 gatgcgcccg ctcgcgtgag tgcgcaagcg gcggctgtag tcgtcgcagt aactcgaagc
     1081 tccggccagg accagcttca cgttgtttgg gagctgggcc tggatctgct cgaaggcgtt
     1141 cacctgcagg tggcagttct tttccggcga aaatctaccg aggaatagaa catagttccc
     1201 gggttcgaga ccccactcca ggatgtggcg ggcttcgcgc cattcgcgca gcactccgcc
     1261 attcggaacg tggaacgtct cgacaccatg cgcctcgcga tagcgatgct gcagcgcccg
     1321 ggaaacgacc atggttccgc tgggaaactt caccgacgct cgctcgcccg cgcgcagcac
     1381 cgccgaggcg agccgtcccc atttcttccg ctgccagtcg agtccctgca cggtgaccgc
     1441 ggttcgagtg cccaccagtc gcgggagaaa ggagaacagc gccgggccga gggcatgata
     1501 gtgcaccacg tcgtacctct gtgtgaaggc gtgggcggtg ctgagcagcg tgtgcaccaa
     1561 cgtttccaga tgcttggacc gaacagtagg caggcggacc acccgcatgc cattccgctg
     1621 ggcaatcggc ggagtgaagt aattacggca gtagactgtg acttcgtgtc ccatctcgac
     1681 cagacgtttg ccgacttcct cgtagtaact ttcgatcccg ctgtactttg agacgagccc
     1741 gcgcccgccg atgaaggcca gccgcagtcg gaggccgggc acgggaagcg ggcgcgggcg
     1801 ccgcagggcg aagatacgac gacgcggcgt aaccggttgg gactgagtgc ctgggaccag
     1861 gcacgctttc gcggtgatca cgctttcgta gaaactgagc agctttcggt agtggccctc
     1921 gggcgtgtgg tggagccgga cgaagtcgaa gccggctcgg cccatctttt gcgccagatc
     1981 ggggttcgat cccagcattt cgatctgaca cgcaagttgg ccgatgtccc ccgtgcgata
     2041 gagcagaccg gtctcgccct cgcgcaccag ttcccggcgc gatcccagat cggaggcgac
     2101 gacggcccgg cccgctgcgt aggactcgag gatcgtcttg cctaaggtct cgtaggcatg
     2161 ggagggcagg accgtgaatt gcgattcagc aatcagcgag tcgcggcgcg aagggccgac
     2221 gtggccgacg aattccacgt tggtcaaacc gagagcagaa gccaggccct gtaacggttc
     2281 gcgctgcggg ccgtcgccgg caataatcag ccgcatctgc ggcgcattct gcatggcgcg
     2341 cagcagatcg tccacgccct tctcggccga gagcctgccg aagtaaagca cggggcggcg
     2401 gtcatcccga gacgccagtt ggcatgcagg cggcgacgag gtctcctgga aatgaggcag
     2461 gatctcgaat ttgccgccgt cccagccgtg ctcgacgaac ttgtcgcgca cgaactggct
     2521 gggcgcgagg aagaggtcga cgcacttgcg gtaggtgccg agcaacttgt gaatgtaggc
     2581 ttctgccgac agcgtgatgc gtgcacctat tcccgggtaa cattcgggcc gcagggcatg
     2641 ccagaaggct ccgcccttgc aggcttcgca cggttcgccg cacgacacca ggttgtagct
     2701 ggggcagagc agcttgaagt cattcacgtg atagacgacc gggacctgct gcgccttcag
     2761 ttcccacagg atcgacggcg acaggtggtg gtagatgttg cggacgtgcg ccacgtcggg
     2821 gcggaagtcc cggatcagag cgcggattcg acggcgcgcg tcgcgcgaat agatcgcatg
     2881 cgccgccaga cgcgccttct gccacggtcc ggcgtctgcc ttgaaatcga tgtgcgggac
     2941 gaggtgtttg tcgtagggcg tcggctcgcc gcgcgggtca gacatcgaaa acagcgccac
     3001 ttcgtggccc tgcgaccgca tcaggttcat cagctcgaac aggtaaactt cggtgccgct
     3061 gaagcggtag ttgtacttgt tgcaatacag gattctcatg aagaaggctc ccggtctgcg
     3121 ggaagatccg ctgaaggaag gtgttggcga acgatcgcag gtttttgctg cggttgaagt
     3181 tgtggcgaat ctggacccgg gccgcgtgcc gcatccagtc gaactggtcg cccacaacgc
     3241 tttccagcga gacttggttc gcggaaagtc cgccgccatg gccctcggct cgccgacccg
     3301 acgcatgcat gagcgaatgg attaacagca ggcgggcgac gaaatcgccc atagagccgg
     3361 gctggtatag aaaaccagtc tgcccaggga tgacgagctc agggactccg gtgatggcgg
     3421 gggcgagcac gatcttgcca cgggccatgg cctccatcag caccaggggg atcccctcgc
     3481 tgcggctggt caataccacc acgtcggcgc ggtcgtagag cgaagccgct tgctcgcgcg
     3541 gcacatgtcc caggagcgtc acccggtctt ccaggccgta gcggcgaatc agggtctcca
     3601 gcttctttcg ctccgggcct tcgccggcaa tggcacattc gaactgcacg tccgtggcat
     3661 gcagttgggc gcaggcacgt atgaggaatg cgtgatcctt cactgcgtgc agacgcccca
     3721 ctgaaagcag gttgagaacg gcccgtggac gctcggctgc gcggggcagg aagttcaggt
     3781 ggtcctcgac ttccacgcca agccgcgaga cgactacttt ttgcggttcc acttcggggt
     3841 attgctcgag aatgaagcgc cggttgtact cggacactgt cagactgaag ctgcagagct
     3901 cgagcttcac gtcgagatag gaaccatgca gcagcaaatc cgagccgtgc agggtcatgc
     3961 tgaagccaat gtcgagcagg cgcgcggcca ccatagcgat ccaggaaccg aaatagccgt
     4021 ggtggacgtg gatgtggtcg acccggtgtt cccgcagcag aatcgcgtag caggcgccca
     4081 gccaggtatg cagcagagcc ttcatgcgct gcaccggcga ctcccgcccg cggcagagca
     4141 cgcgccagag caggtctgat actcgcggcc atctccgaag gcagagccaa agcgcctgga
     4201 gcagaacaac ggcgcgcagc gggaacaatg tgatgtcggg cgaattgccc gcaacgagaa
     4261 cctcctccgt ggccggctcg cgcacactcc ctgcaatcac gcggactcca cgcttccgca
     4321 gttcgccgat ctcctccacc acgtaaggct cgactggaga aggaaatcga ttcgcgagat
     4381 aggccacggt cagcatggct gagctgactc cgcccgccgc tgctgcgccg ccagcatccc
     4441 ggcaagcgtg a
//
LOCUS       DASPUL010000176         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_33193, whole genome shotgun sequence.
ACCESSION   DASPUL010000176 DASPUL010000000
VERSION     DASPUL010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33193"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..494
                     /locus_tag="VEJ00_04225"
     CDS             192..494
                     /locus_tag="VEJ00_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXX00396.1"
                     /translation="MPLYEYQCKSCHSLTERIQKFSDPPLTICPHCGGELEQLISAPA
                     VQFKGGGWYVTDYAKKSHAPAASDGAAKDSKESKKDDKKPESAAKESSAKKADKHK"
     gene            complement(650..>2812)
                     /locus_tag="VEJ00_04230"
     CDS             complement(650..>2812)
                     /locus_tag="VEJ00_04230"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXX00397.1"
                     /translation="TQTQPAPVKPLTIEAIYQPGGLTGRGPENVAWSPDGTKLSFVQR
                     NAAGNEGELWEVDAATGDKKILVNAAKLAALVPDVNNVKNERERERLTRYHVAAYQWA
                     PDSKRMIFDSFGQLWLYDLGSQTAVQFTSSPEASENPRFSPDGSHVAYVRKHNLYVHS
                     VDGKGERQLTHDTSDNLLNGEIDWVYAEELSVRSNYFWSPDSKEIVFLHMDETNVPTH
                     PLVDLLPTHATVDLQKYPQVGDPNPAVKLGVVDAEKGKVRWISLSSDEDIYIPRFGWA
                     GDGVIWAEVLNRAQDHMDLYFVDAKSGKSRIVLTESTPGAWIDFDHVEVRFLAAGRFL
                     WPSWRDGNMHLYLYSFDAKNPLAADAKLERQLTQGDFEVLGIEGVDEAAGTVFFAANK
                     DDPREVHIYSVQLDGSGFKVLTPEEGEHLADFSDDGKHYLHTFSGPTTEPTVSLCAVG
                     GSCSAVWRSPDLVAEYGLRAPQYLEFKAEDGTVLHGRLLLPPDTPAGGKIPLIVHIYG
                     GPAHQMVTKIPPDPFDEILARKGFAVFAVDNRGTPGRDRKFQTAIRHEFGAVELKDQL
                     TALDQLLAQYPQLDKDRIAIWGWSNGGSMTLYAMTHSDRFRAGVAVAPVVDSRNYDSI
                     YMERYLGLLKDNKEAYEQSNVALSAANLHGALLLAHGTGDDNVHFQNSVQMIEALIKA
                     GKQFRLMIYPNKTHSISGKDARVHLFEMITEHFERELK"
BASE COUNT          549 a          874 c          789 g          600 t
ORIGIN      
        1 cgtcacgcaa gtgacttttt ccgcggccgg taaagcccag gtctctcgcc tgctatggac
       61 ggcctgaagg ccgtgccctt ccatgccgaa actcgactag cgttcccaaa cgcccgttcg
      121 aattcgcatt gacctggccc aatggtgcct aatagaatca atcggttata cctacctccg
      181 ggaaaactgc tgtgccgcta tatgaatatc aatgcaagag ctgccatagc cttacggagc
      241 gcatacaaaa gttctccgat ccgcctctga cgatctgccc gcattgcgga ggcgagcttg
      301 agcagttgat ttccgctccc gccgtgcagt tcaaaggtgg cggctggtac gtgaccgatt
      361 acgccaagaa gtcgcacgcg cccgcggcgt cggacggcgc ggctaaggat tccaaagagt
      421 caaagaagga cgacaagaag ccagagagcg ccgcgaaaga aagttccgcc aagaaagccg
      481 acaagcacaa gtaggtgcca accatctctt gtcatctgag gcacgcattt gcgccggcgg
      541 atctatctgc ttgttggcac tactcgcgct gtcggcaggt tgcacagatc cttcgcttcg
      601 ctcaggatga caaggcaact cgattgcttc agctgacaag tcaactctgc tacttcagct
      661 cccgctcaaa atgctccgtg atcatttcga acagatgcac gcgcgcgtct ttgcccgaga
      721 tgctgtgcgt cttgttggga tagatcatca gccgaaactg tttgccggcc ttgatcagcg
      781 cctcaatcat ctgcacgctg ttctggaagt gtacgttgtc gtcgcccgtg ccgtgcgcca
      841 gcagcagcgc gccgtgcaag ttggcggcag acagtgctac gttcgattgt tcgtacgctt
      901 ccttgttgtc cttgagcaag cccaggtagc gctccatgta gatggagtcg tagttgcgcg
      961 aatccaccac cggagccacg gccacgccgg cgcgaaagcg gtcggaatgg gtcatcgcgt
     1021 acaaggtcat cgatccgccg ttcgaccatc cccagatcgc gatgcggtct ttgtcgagct
     1081 gcggatattg tgccagtaac tgatcgagcg ccgtgagctg atccttcaac tcgaccgcgc
     1141 cgaactcgtg ccggattgcg gtctggaatt tgcgatcgcg ccccggggtg ccgcgattgt
     1201 ccactgcaaa caccgcgaag cccttgcgcg cgagaatctc atcgaacgga tcgggcggga
     1261 tttttgtcac catctggtgc gccggaccgc cgtagatgtg gacgatcagc ggaatcttgc
     1321 cgcccgcggg ggtgtcggga ggaagcagca gccgtccgtg cagaacggtc ccatcttctg
     1381 ccttgaactc caaatactgg ggcgcacgca gtccgtactc ggctactagg tcgggcgagc
     1441 gccacactgc gctgcacgac ccgcccaccg cgcaaagcga aaccgtaggt tccgtcgtgg
     1501 gcccagagaa tgtgtgcagg tagtgcttgc cgtcatcgct gaagtctgcc aggtgttcgc
     1561 cttcctcggg cgtgagaact ttgaaccccg atccatccag ttgcaccgag tagatgtgca
     1621 cctcccgcgg gtcgtccttg ttcgcagcga aaaataccgt ccccgcagct tcatccacgc
     1681 cctcgatgcc cagcacttcg aagtcgccct gcgtgagttg cctttccagc ttcgcatcgg
     1741 ctgcgagcgg gttcttcgcg tcgaagctgt agaggtacag gtgcatgttg ccgtcgcgcc
     1801 agctcggcca caggaaacgt cccgcggcga gaaaacgaac ctccacatgg tcgaagtcga
     1861 tccacgcgcc gggcgtggac tcggtcagca cgattctgga tttgcccgat ttcgcatcca
     1921 cgaaatacaa gtccatgtga tcctgcgcgc gattcagcac ctcagcccag atcactccgt
     1981 cgcccgccca gccaaaacgc ggaatgtaga tatcctcgtc gctgctcaac gaaatccagc
     2041 gcactttgcc cttttccgca tcgaccaccc ccagcttcac cgctgggttc gggtcgccca
     2101 cctgcggata tttctgcaga tcgacggtcg catgggttgg cagcaagtcc acgagaggat
     2161 gcgtcggaac attggtctcg tccatgtgaa ggaacacaat ctctttgctg tcgggcgacc
     2221 agaagtagtt gctgcgcacc gacagctctt cggcgtacac ccagtcgatc tcgccattca
     2281 ggagattgtc gctggtatcg tgagtaagtt gtctttctcc cttgccatca acggagtgga
     2341 cgtaaagatt gtgcttgcgc acgtaggcca catggctgcc gtcgggcgag aacctgggat
     2401 tttcgctcgc ctccggcgac gaagtaaact gcaccgcagt ctgcgatcca aggtcgtaga
     2461 gccagagctg cccgaatgaa tcgaagatca tgcgcttcga atccggcgcc cactgatacg
     2521 ccgccacgtg atagcgggtc agccgctccc tctctcgctc gttcttcacg ttgttgacgt
     2581 cgggaacaag cgcggccagc ttcgccgcgt taacgaggat cttcttgtca ccggtggctg
     2641 cgtctacttc ccagagttcg ccctcgtttc ccgcggcgtt gcgctgcaca aacgacagtt
     2701 tggtgccatc cggactccac gccacattct ccggacctcg ccctgtcagc ccgcccggtt
     2761 gataaatcgc ttcgatggtc agcggcttga caggtgccgg ctgcgtttgt gt
//
LOCUS       DASPUL010000177         3200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_33249, whole genome shotgun sequence.
ACCESSION   DASPUL010000177 DASPUL010000000
VERSION     DASPUL010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33249"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VEJ00_04235"
     CDS             complement(<1..312)
                     /locus_tag="VEJ00_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00398.1"
                     /translation="MTLALTLLVVVSSVPAAPPLKAPPAQSAAQTTTTVPPQGATKAE
                     DQGAVPAQNPSPASKSDQASSSAQTSSTQNQPAPPKRPRRKKKPKNVNCVLPPATADS
                     TT"
     gene            complement(416..2287)
                     /locus_tag="VEJ00_04240"
     CDS             complement(416..2287)
                     /locus_tag="VEJ00_04240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXX00399.1"
                     /translation="MLRRKAIIVLVITFMVTVMVSAFSYLYVSQILRLRILNAYETAT
                     NLTHQLSYAAANAVPDFSSTRLDTSDPAAVRRAVADSLQVDIDLNNLMQSDPGDWSYI
                     YDVTIVDTSGKALLHTNAAMVGKVVPPRPDFQRLMGARFPDQVRMVFSPATVYEVSYP
                     LQVSGEPFGTIRVGVQTLFLKNEVATRLIKAAYFSVASIFLSLLLAAGISNLALGPLR
                     EISRNLDSVSGGETLSGDDSEHDEYGLVTLKIANLGRQIRDSKEIFSALKDNVDQLMS
                     KLQDGLMLFTRDSRVVLVSAPVEAFLGRPRAELLGRSVQEIFDRHSLLGTALLDAFER
                     RRPLSQREFEVAGGKTVQVSLDFVQEKNSQIGALLIMRDTESVRRIGDEIEMSRRLSA
                     SGRLTRGVAHEVKNPINAIVLHLQLLRNKLAHQEPDTRRHMDIIDSEIRRLDRVVQTL
                     VDFTRPRDLRLEEIDMRRVMEEVAQLAAPDAEQHGVKIERHMPAERLPIKVDMDLMKQ
                     AILNVVINGVQAMPRGGVLAISAHREDNLVVAEVRDQGGGIPPDMHDKIFELYFTTKK
                     DGSGIGLAQTYQILQWHYGTVDFESTEGVGTTFRFHIPVAGPAREAGHAYAADVAAS"
     gene            complement(2333..>3200)
                     /locus_tag="VEJ00_04245"
     CDS             complement(2333..>3200)
                     /locus_tag="VEJ00_04245"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXX00400.1"
                     /translation="ARTIHELSPRKERPFVAINCAAIPETLMESEIFGHEKGAFTGAL
                     ERRTGCFELAEGGTLLLDEIGEMPVGTQAKLLRVLEDRKLRRLGSKVETPVDVRVLAA
                     TNKIPDEAVSKGELRSDLYYRLNVFNIHMPPLREHKDDIVGLVQQLLTEMSSKHGRKV
                     VAVSEAVLNQFNNYSWPGNVRELRNTIERAVIVCDGAMIETRHLPPGFGQAPLRAAAN
                     DPDAVRLGVGTTVEEAEKMLILKTLEATSNNKTRAAEILGISLKTLHNKLKEYGAAVA
                     EASAGKEEVSSG"
BASE COUNT          600 a          928 c         1006 g          666 t
ORIGIN      
        1 cgtcgtcgaa tcggccgttg ctggagggag cacgcagttg acgttttttg gcttcttctt
       61 gcgacgtggg cgtttgggag gcgcgggctg attctgggtt gacgaagtct gggcagacga
      121 agaagcctga tcggacttgg aagcaggaga gggattctgg gcaggaactg ctccttgatc
      181 ctcagccttc gtggcgccct gcggcggaac agtcgtagtt gtttgggctg cagattgcgc
      241 gggaggcgcc ttcagcggtg gcgcagccgg tacgctggaa accacgacga gtagcgttaa
      301 ggccagggtc atgcgcggat ctggagttca gcgcccaaag gctaatctta cgcctttcaa
      361 gggcgaagca ggagagttga tggtctccga ggtcatcccc acgagcgccc cggggttatg
      421 aggccgccac gtcggcggca taggcgtgac cagcctcccg cgccggtcct gccaccggaa
      481 tatggaagcg gaaggttgtg ccgacgcctt cggttgattc aaaatccacc gtgccgtagt
      541 gccattgcag gatctggtaa gtctgcgcca ggccgatgcc actgccgtct ttcttggtcg
      601 tgaaataaag ctcgaaaatc ttgtcgtgca tatcgggcgg aatcccgccg ccctgatcgc
      661 ggacctcggc gaccaccagg ttgtcctcac ggtgtgcgga gatggcaagc acgccgcccc
      721 ggggcatggc ctgaacgccg ttgatgacga cgttgaggat ggcctgcttc atcagatcca
      781 tgtcaacctt gatcggcaaa cgctccgccg gcatgtggcg ctcgatcttg acgccgtgct
      841 gctcggcgtc gggagcggcg agttgcgcca cttcttccat cacgcggcgc atgtcgattt
      901 cttccaggcg caggtcgcgg ggccgggtga aatccaccag cgtctgcact acgcgatcca
      961 gtcgccggat ctcgctgtca atgatgtcca tgtggcggcg agtgtcgggc tcctgatgcg
     1021 ccagcttgtt ccgcagaagc tgtaagtgca gcacaatggc gttgatggga ttcttcacct
     1081 cgtgagcgac gccgcgcgtg agccggccgc tggccgagag gcgtcgcgac atctcgattt
     1141 cgtcgccgat gcggcgaacc gactccgtgt cgcgcatgat caggagcgcg ccgatctgcg
     1201 agtttttctc ctgaacgaag tcgagcgaga cctgtacggt tttccctccc gccacctcaa
     1261 actctcgttg cgaaaggggc cggcgacgct cgaaggcgtc gagcaaagcg gttccgagca
     1321 gcgagtgccg gtcgaagatc tcctggaccg agcgccccag cagttcggct cggggacgtc
     1381 cgaggaatgc ttccacgggg gcgctgacca gcaccacgcg cgagtcgcgc gtgaacagca
     1441 tcagaccatc ctgcaacttt gacatgagct ggtcgacgtt gtccttgagg gcggagaaga
     1501 tttccttgct gtcgcgaatc tggcgcccca ggttcgcgat cttaagagtc accaatccgt
     1561 attcgtcgtg ttcggaatcg tcgcccgaga gggtctcgcc gccgctcacg ctgtcgaggt
     1621 tgcggctgat ttccctcagc ggtcccagtg ccaggttgga gatgcccgcg gccaatagca
     1681 aagacaggaa aatagacgcg actgaaaaat aagccgcctt gatgagccgg gtcgctacct
     1741 cgttcttcag aaacagcgtt tgcacgccga cgcgaatcgt gccgaatggc tcgccgctca
     1801 cttgcaacgg atagctcact tcatagacgg tggccgggct gaaaaccatg cgaacctggt
     1861 ccgggaagcg ggcgcccatc aaacgctgga aatccggccg tggtggaacc accttcccca
     1921 ccatcgcggc attggtgtgg agcagagctt tgccgctggt gtcaacgatg gtgacgtcgt
     1981 agatgtagct ccaatccccc ggatcagact gcatcaggtt gttcaggtcg atatccacct
     2041 gcagtgagtc cgcgacagcc cgccggacag cggccggatc gctggtgtcg aggcgggtac
     2101 tgctgaagtc agggactgcg ttggctgcgg cataggacaa ctggtgagtc aggttcgtcg
     2161 cggtttcgta agcatttaag atacgcaagc ggagaatctg ggaaacgtaa aggtaggaaa
     2221 acgctgacac catcactgtc accatgaagg tgatgacgag aacgatgatg gcctttcgtc
     2281 gcagcatgac cggagccgga taaggcaggg cgcagaacag tgcaaagcgg tcctaaccgc
     2341 tggatacctc ctccttgccg gcagatgcct cggcgacggc ggcgccatat tctttcagct
     2401 tgttatgaag tgtcttcagg ctgatgccaa gaatctccgc cgctcgcgtt ttattgttgc
     2461 tggtggcctc cagcgtcttc agaatcagca tcttttcagc ctcttcgacg gtagtgccca
     2521 cgccgaggcg aacggcatcc gggtcgttag ctgccgcgcg caacggcgct tgtccgaatc
     2581 cgggcggcag atgcctggtc tcgatcatgg cgccatcgca gacgatgacc gcccgctcaa
     2641 tcgtattgcg cagttcgcga acgttgcccg gccaggagta gttgttgaac tggttcagca
     2701 ctgcctcact gaccgcaacc accttgcgcc cgtgtttgct gctcatttcg gtgagcaact
     2761 gttgtaccag gccgacgata tcgtctttgt gctcgcgcaa aggaggcatg tgaatgttga
     2821 aaacgttgag acggtaatac aggtcgctgc gcagctcacc tttggacaca gcttcgtcgg
     2881 ggatcttgtt ggtagccgcg agcacgcgaa cgtcaaccgg agtttcgacc ttgctgccca
     2941 gccgacggag tttccggtcc tccagcaccc gcagaagctt ggcctgggta ccgaccggca
     3001 tttccccgat ttcgtccagt aaaagggtcc ctccctcggc cagttcgaag cagccggtgc
     3061 ggcgttcgag cgctccggtg aaggcgcctt tttcgtggcc gaagatctcg ctttccatca
     3121 gtgtctcggg aatggcggcg cagttgatgg cgacgaaagg cctctctttt cgagggctga
     3181 gctcgtgaat cgtacgggcc
//
LOCUS       DASPUL010000178         3239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_33302, whole genome shotgun sequence.
ACCESSION   DASPUL010000178 DASPUL010000000
VERSION     DASPUL010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33302"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..792)
                     /locus_tag="VEJ00_04250"
     CDS             complement(28..792)
                     /locus_tag="VEJ00_04250"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXX00401.1"
                     /translation="MKLSVVMPVYNEQATLREVIARVLAVPLEIELICVDDGSSDQSR
                     EILAEQQAIRPQIRVLLQPKNMGKGAALRRGIHESTGDYVIIQDADLEYDPAEYSALL
                     RPLIEGKADVVYGSRFLGGGPHRVLYYWHSVANWGLTLISNTLTNLNMSDMETCYKVF
                     RRDVIQSIPIEEDRFGFEPEITVKVAKRGLRVYEVGISYWGRTYEEGKKIGWRDGFRA
                     LFCLLKYTLTEPRVSPLPDASRAVSASADWDEQRSR"
     gene            complement(923..1909)
                     /gene="truB"
                     /locus_tag="VEJ00_04255"
     CDS             complement(923..1909)
                     /gene="truB"
                     /locus_tag="VEJ00_04255"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXX00402.1"
                     /translation="MNGVVIIDKPAGFTSHDVVNRVRRILGQRSVGHLGTLDPMATGV
                     LPLVIGNFTRLAQFYTKAAKTYEGTIRFGFATDTYDAEGEAFGAAQEVSLRTEDVEAL
                     ATQFRGLIEQTPPPFSAKKIHGVPAYKLARRQKDVVLKPVQVEIKEFHILAVEGDRVR
                     FTARVASGTYLRSVAHEMGQRLGCGAHLESLRRTAVAEFTLAEAHTLDELAQRCESNR
                     RRAIPEGTGTGGPGIPATTTVAAPFTAESLFIHPRKLLPELPCVTTDELTAARIRSGR
                     TVNLPDLSRARQIKVFCGQTDLLAIATRVAGTLFHPAIVFSVSGHDEEKGRG"
     gene            1989..2687
                     /locus_tag="VEJ00_04260"
     CDS             1989..2687
                     /locus_tag="VEJ00_04260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXX00403.1"
                     /translation="MLPIKDDQPRYSTPYINSFLIGLNVLVFLFEATLGPSDLKTLIH
                     QFAVIPSHVGAFLAGSPRYPLPAILVPFFTSMFLHGSWMHVLGNMWFLYIFGDNVEDY
                     LGHFTYLIFYLLAGLFAMGVQTAVDLHSTIPTLGASGAIAGVMGAYLILYPKARVLTW
                     FPLIFFFYLPAWVMLGYWFVLQFFSGAATALTYEGKSTGGVAFWAHVAGFIAGAVMIK
                     IFPERGRRSPYAYR"
     gene            complement(2763..>3239)
                     /locus_tag="VEJ00_04265"
     CDS             complement(2763..>3239)
                     /locus_tag="VEJ00_04265"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX00404.1"
                     /translation="TPVKPADSPSMVVLDSWNDIGRKLIDMAEDFPEDKYEMKPTPAQ
                     RTFAQQLLHAAGANYYFTNLLTGSKSPVDENPKYTSKADVVAFVKKSFADGAAAIQAK
                     GDKGMNDLVVDPFAHQQTRVYDMAYGIIEHSGEHYGQLVGYYRLAGLVPPESRPKK"
BASE COUNT          598 a          948 c          995 g          698 t
ORIGIN      
        1 tgtggaccga tcgcggcttg aagcgagtta ccggctgcgc tgttcgtccc aatcagcgga
       61 agcggagact gctcttgaag catcaggaag agggctgacc cggggctcgg tgagggtgta
      121 cttcagcagg cagaacaggg cacggaagcc gtcgcgccaa ccgatcttct tcccttcctc
      181 gtaggtacgg ccccaatagc tgatgccgac ctcgtagacg cgcaggccgc gcttggcgac
      241 cttcactgtg atctccggct cgaagccaaa gcggtcctcc tcgatcggga tcgactggat
      301 cacgtcgcgg cggaacacct tgtaacaggt ttccatgtcg ctcatgttga ggttcgttag
      361 cgtgttcgag atcagagtca ggccccagtt ggccaccgaa tgccagtagt agagcacgcg
      421 gtgcgggccg ccgcccagaa agcgcgagcc gtaaactacg tctgcctttc cctcgataag
      481 cggccggagc agcgcagaat actcggcggg atcgtattcc agatcggcgt cctggatgat
      541 gacgtaatcg ccggtcgact cgtggatgcc gcgacgcagg gctgctccct tgcccatgtt
      601 cttgggctgc agcagcaccc ggatctgggg gcgtatggcc tgttgctcgg cgagaatttc
      661 gcgcgactga tcgcttgagc cgtcatccac gcagatcaac tcgatttcga gcggcacagc
      721 gaggacccgc gcgatcacct cgcgcagcgt ggcctgctca ttgtagaccg gcatgacaac
      781 cgacaacttc atgggctagg gccacagtaa aggccccggg aggtcgcgtc aagcgggaca
      841 gcccttttcg ggcgggcttt ttctcaaaac agaaccggcc tcgaaatcct gtggcctggc
      901 tagtcaagct agagcccggc gatcagcccc ttcccttctc ctcgtcgtgt cccgagaccg
      961 aaaaaactat ggccggatgg aaaagcgtgc cggcaacacg cgttgcgatc gccaggagat
     1021 cggtttgtcc acagaagact ttgatttggc gggcgcgaga gaggtcggga agattgaccg
     1081 tgcggccact gcggatgcgc gcagcagtga gctcgtcggt tgtgacgcag ggtagctcgg
     1141 gcaggagttt acggggatga ataaatagcg attccgctgt aaagggggcc gctacggttg
     1201 tagttgcggg aataccaggt ccgcccgtcc cagttccctc cgggatcgct cgtctcctgt
     1261 tggattcaca ccgctgggcg agctcatcca gagtgtgcgc ctcagcgagc gtgaactcgg
     1321 caacggcagt gcggcgcaac gactcgagat gtgccccgca gccgaggcgc tgccccattt
     1381 cgtgggccac cgagcgcaga taggtcccag aggcgacccg agccgtgaac cggactcggt
     1441 ctccttcgac tgccaggatg tggaattcct tgatctcgac ttgtacgggc ttgagcacaa
     1501 cgtctttctg cctgcgcgcc agcttgtagg cgggcacgcc gtggatcttc ttggccgaaa
     1561 acggcggcgg ggtctgctca atgagtccgc gaaattgcgt tgccagggct tcgacatcct
     1621 cggttcgcaa actgacctct tgcgcagcgc cgaaggcttc gccttcggcg tcgtaggtat
     1681 ctgtggcgaa gccgaaacgg atggtgcctt cgtaggtttt tgcggctttg gtgtagaact
     1741 gcgccaggcg cgtaaagttg ccgatgacca gcggtaggac cccggtggcc atcggatcga
     1801 gcgtgccgag atggccgacg gaacgctgcc ccaggatgcg ccggacacgg ttgaccacgt
     1861 cgtgcgaggt gaagccggcc ggcttgtcga tgataacgac gccgttcatg taggattggg
     1921 taactgcgta accgggcagc cggaaaattg cacagcgagt atccggttac caagttacca
     1981 aatctcgcat gctcccgatc aaagacgacc agccacgtta cagcacgccc tacatcaaca
     2041 gcttcctgat tgggctgaat gtcctggtct ttcttttcga ggccacactt ggtccctcgg
     2101 acctgaaaac cctgattcat caatttgcgg tcattccctc gcacgtaggg gcgtttcttg
     2161 cgggatcacc gcgctatccg ctgccggcca tcctagttcc cttcttcacc tccatgtttc
     2221 tgcatggctc gtggatgcac gtgctgggca acatgtggtt cctgtacatc ttcggggaca
     2281 acgtggaaga ttatctcggg cactttacct acctcatttt ctacctgctg gcggggctgt
     2341 tcgccatggg ggtgcaaacg gcggtggatc tgcactccac catacccacg cttggagcaa
     2401 gtggcgcgat tgcaggcgtg atgggagcgt atctgatcct ctatccgaag gctcgcgtgc
     2461 ttacctggtt ccccctgatt ttcttctttt acctgccggc ctgggtcatg ctgggttact
     2521 ggttcgtgct gcaatttttt agcggggcgg ccactgccct tacttacgag ggaaagagca
     2581 ccgggggagt tgcattttgg gcgcacgtag cggggttcat cgccggcgcg gtgatgatca
     2641 agatcttccc tgagcgtggg agaagatcac cctacgccta ccggtagcga tgatgaagtc
     2701 aaaatcccca ccctaccaaa atcgggtagg gtggggcacc ctccaggttc tcgattcggc
     2761 gctcacttct tcgggcgcga ctccggcggc accagtccgg ccagccggta gtagcctacc
     2821 agttggccgt aatgctctcc cgagtgctcg atgataccgt acgccatgtc gtaaacccgc
     2881 gtttgctgat gggcgaaggg atcgaccacc agatcgttca ttcccttatc gcccttggcc
     2941 tggatggcgg cagcgccgtc ggcgaaagac ttcttgacga acgcaaccac gtccgccttg
     3001 ctcgtgtatt tcggattctc gtcgaccggg ctcttgctgc cggtgaggag attcgtgaag
     3061 tagtagttgg caccggcggc gtgcaacaac tgctgggcga aagtgcgctg ggccggtgtg
     3121 ggcttcatct catacttgtc ttcggggaaa tcctcggcca tgtcgatcag ttttcgccca
     3181 atatcattcc agctgtcgag cacgaccatc gaagggctgt cggcgggttt gaccggggt
//
LOCUS       DASPUL010000179         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1261722, whole genome shotgun sequence.
ACCESSION   DASPUL010000179 DASPUL010000000
VERSION     DASPUL010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261722"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1859)
                     /locus_tag="VEJ00_04270"
     CDS             complement(<1..1859)
                     /locus_tag="VEJ00_04270"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HXX00405.1"
                     /translation="MPSLRVILLWHQHQPFYKDLVTGEYRLPWTRLHALKDYYGMVKL
                     LDEFPNVHQNFNLVPSLMVQIQDYVAGTAHDPFLSVAAKPARDLSVEERRFALQYLFQ
                     ANPQNVIGRYPRYRELWERFREHGDHPERAERYFQLQDFTDLQVLSQIAWFDEFFLEE
                     KDVAALVAKGRSYSLDEQKFVIARERELLGKVLPAYADAAKKGSIEISATPFYHPILP
                     LVCDTTAGAVSSPGLPLPQNRFRHPEDAREQLVRALDLHEKVFGLRPKGVWPSEGSLS
                     EEVLAIAHSLGIQWMATDEGVLGRTSGVFFARDGNGRLPAHLAERLYNIHRYESSSAA
                     MHLVFRDHTISDLIGFVYSGMPPNDAAQHLLGNIKEAAKPVLANGRDAVVSIILDGEN
                     AWEYYPKSGREFLRRFYDAVQREPGVEAVTISEAIARHKDFGKLTSLVPGSWINANFN
                     VWIGAPEDNRAWDYLHHAREFYAQNAARAGESQRKLAFDEILIAEGSDWNWWYGPEHH
                     SANDRDFDELYRKHLSNVYQALGATPPDYLAQPITGAEVRPSFVPQTAYIHPRVTGDK
                     VRYFEWLGAAVYTADHRAGAMHGKQFLLDSVYAGIDATHVYGRLDFKDKVPGA"
     gene            2082..2438
                     /locus_tag="VEJ00_04275"
     CDS             2082..2438
                     /locus_tag="VEJ00_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00406.1"
                     /translation="MRRYHHIGIPTAESKPGETHLKHLKVFVVSHQKSEFGVEWMRFE
                     ADAAVPDLVRRVPHVAFEVTDLSSELAGREILIPPNSPSDGVRVAFIVENGAPIELLE
                     FTDPKHPARLGDQMVE"
     gene            2552..>3546
                     /locus_tag="VEJ00_04280"
     CDS             2552..>3546
                     /locus_tag="VEJ00_04280"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXX00407.1"
                     /translation="MALTSGTKLGPYEILSPVGAGGMGEVYRARDTRLGRDVAIKVLP
                     EAAAHDADRLRRFEQEARTVAGLNHPNILGIHDIGTHDGAPFLVSEFLEGQTLHETLV
                     SGPLPLRRAIEYALGLAQGLAAAHEKGIVHRDLKPENVLITRDGRVKVLDFGLAKLVR
                     PEESREAVVTLTSPATLPGMVMGTVGYMSPEQVRGGASDARSDIFSFGAVLYEMLTGK
                     RAFKRDTSAETMTAILREEPPELIDTGWQAPLELQRILGRCLEKSAERRFQSASDLAF
                     AIESLSGTSAARSIEAPKARRAWVPWAAVALLVVAFGAAVWSVGRWSAAHPPLKFT"
BASE COUNT          712 a         1032 c         1131 g          671 t
ORIGIN      
        1 tccgctccgg ggactttgtc tttgaagtcc aggcggccgt acacgtgcgt ggcatctatg
       61 ccggcgtaaa ccgaatccag caggaattgc ttgccgtgca tggcgccggc gcggtggtcc
      121 gccgtgtaaa cggccgcgcc cagccactcg aagtagcgga ccttgtcacc tgtaacgcga
      181 ggatggatgt aagccgtctg tggcacaaac gaggggcgca cctcggcacc agtaatgggc
      241 tgcgcaagat aatccggagg agtggcaccc agcgcctgat acacattcga cagatgcttg
      301 cgatacagtt cgtcaaaatc ccggtcattg gctgagtgat gctcggggcc ataccaccag
      361 ttccagtcgc tgccctcggc gatgaggatt tcatcgaacg cgagcttgcg ttgggactcg
      421 ccggcccgcg cggcattctg ggcatagaac tcgcgcgcat gatgcaggta gtcccaggcg
      481 cgattgtcct ccggcgcacc aatccagacg ttgaagttgg cgttgatcca ggagccgggg
      541 acgagcgaag tcagcttgcc gaaatctttg tgccgggcga tcgcttcgga aatcgtgacc
      601 gcctcgactc ctggctcgcg ctgcacggca tcgtagaagc ggcgcaggaa ctcgcggccg
      661 gacttcgggt agtattccca ggcattttcg ccgtcgagga taatcgagac gacggcatcg
      721 cggccgtttg caagcaccgg cttggctgct tccttgatgt tgccgagcag atgctgcgcg
      781 gcgtcgttcg ggggcattcc ggagtagacg aatccgatga ggtcggaaat cgtgtgatcg
      841 cggaagacca ggtgcatggc cgccgagctg ctttcgtagc ggtggatgtt gtacagccgc
      901 tcggccagat gtgctggtag gcgtccattt ccgtcgcgag caaaaaatac gccggaagtg
      961 cggccaagca ccccttcgtc ggtcgccatc cactgaatgc ccagactgtg cgcgatggcg
     1021 agaacttcct cggagagact tccttccgac ggccacactc ccttcgggcg caagccgaag
     1081 actttttcgt gaaggtcaag ggctcgcacg agctgctcgc gcgcgtcttc gggatggcga
     1141 aagcggttct gcggcagcgg caggcccggc gacgacacgg cgcctgcagt tgtgtcgcag
     1201 accagtggca gtatcgggtg atagaacggc gttgctgaga tttcaatcga gccttttttc
     1261 gcggcgtcag cgtaggcggg cagcaccttg cccagcagtt ccctctcgcg cgcaatcacg
     1321 aacttttgct cgtcgagcga gtagctgcgt cccttcgcga ccaacgctgc cacgtctttc
     1381 tcttccagaa agaactcgtc gaaccacgcg atctgcgaca agacctgcag gtcggtgaaa
     1441 tcctgcagct ggaaatatcg ttccgcccgc tcggggtggt cgccgtgctc gcgaaagcgc
     1501 tcccacaatt cgcgatagcg ggggtagcgt ccgatgacat tctgcgggtt ggcctgaaac
     1561 aggtattgca gggcgaagcg gcgctcctct accgacaaat cccgggccgg cttggcggcg
     1621 acgctgagaa aggggtcgtg cgcggtacca gcgacgtaat cctgaatctg aaccatcagc
     1681 gacggcacga ggttaaagtt ctggtggacg ttggggaact cgtcgagcaa cttcaccatg
     1741 ccgtaatagt ctttaagggc gtgcaggcgg gtccaaggca ggcgatattc acccgtgacc
     1801 aggtctttgt aaaacggctg gtgctggtgc cacagcagga tgacacgcaa ggacggcatg
     1861 gctggaaaac gtcaggatag cacgaggatt cagcagtcgc ttccgcgaaa cacgaggtcc
     1921 agactcagct tccgtattgc cgactactcg tccaattggt ctcagcagtt cgggaagtgc
     1981 cactcaggcc ggcccagttt gtgtttcgga aggctcggtg cgattcaaac gcgataacga
     2041 atagagtaga attgtgcgcc cattctcccg gagagaaaac aatgcgccgg tatcaccaca
     2101 tcgggattcc cactgccgag tcgaagcctg gagagaccca cttgaaacac ctgaaagttt
     2161 ttgtcgtcag tcaccagaaa agcgaattcg gcgtggaatg gatgcgcttc gaagcggacg
     2221 cggcggttcc cgatcttgtc cggcgagtgc cgcacgtcgc gtttgaggtg accgacctct
     2281 cctccgagct tgctggccgc gaaatcctga ttcctccgaa cagcccgtcc gacggggtcc
     2341 gcgtcgcatt catcgtcgaa aacggagcgc ccatcgagct gctcgaattt accgacccca
     2401 aacacccagc ccgcttaggg gaccaaatgg tcgagtgacg ggcaaaccag aagtcatcgg
     2461 aacagatcgt cagtttcttg ttggccgacc aaccgtccgg agatcggtac gcatcgactg
     2521 caatgaatac gggctataat tttgcgcatc gatggcgctt acctcgggca ctaaactcgg
     2581 gccgtatgag atcctgtcgc cagtaggagc gggcggcatg ggcgaggtgt accgcgcccg
     2641 cgacacgcgg ctcgggcgtg atgtcgcgat caaggtgctg ccggaggccg cggctcatga
     2701 tgccgaccgc ctgcgccgtt tcgaacagga agcgcgcaca gttgccggcc tgaaccatcc
     2761 gaacatcctg ggaatccacg acattggtac gcacgacggc gcgccgtttc tggtcagcga
     2821 atttctcgaa ggacaaacac tgcacgagac gctcgtatcc ggtccgctgc cgctgcggcg
     2881 cgcgatcgaa tacgcgctcg gcttggcgca gggactcgcg gcggcgcacg agaaaggaat
     2941 cgtgcaccgg gatctgaaac cggaaaacgt gctgatcacg cgggacggac gggtcaaagt
     3001 gctggatttc gggttggcga agctggtgcg gccggaggaa agccgcgaag ccgtggtgac
     3061 actcaccagt cccgcaaccc tcccgggcat ggtgatgggc accgtcggct acatgtcgcc
     3121 ggaacaggtg cgaggcgggg caagcgacgc acgatcggat attttcagtt ttggcgcggt
     3181 gctgtacgaa atgctcaccg gcaaaagggc gttcaaacgg gatacgtctg cggaaacgat
     3241 gaccgcgatt ttgcgcgaag agccgccgga actgatcgac accggatggc aagccccgct
     3301 ggaactgcag cggattctcg gccgctgcct ggaaaagagt gccgagcggc gattccagtc
     3361 ggccagcgat cttgcgtttg ccattgaatc gctgagcgga acctccgcgg cacgcagcat
     3421 cgaggcacct aaggcgcggc gggcctgggt gccctgggca gcggtggcgc tgcttgtggt
     3481 ggctttcgga gcagccgtgt ggtcggtagg ccggtggtcc gctgcacatc caccgctgaa
     3541 attcac
//
LOCUS       DASPUL010000180         3581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_851560, whole genome shotgun sequence.
ACCESSION   DASPUL010000180 DASPUL010000000
VERSION     DASPUL010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851560"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..976)
                     /locus_tag="VEJ00_04285"
     CDS             complement(230..976)
                     /locus_tag="VEJ00_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HXX00408.1"
                     /translation="MSVEIDVLDPTHPKRVAIIVSNPATSKQTGWPIGFWWGEVTHPY
                     WEFTQHGYQVEIFSPDGGRLEGDKWSDPRDDSQYSADDLISMGFIHAPATARQIEQSK
                     PMKDLKVQDFDAVLFVGGQGPMYTFFDDERVHKLAMQFYESGKVLAVICHATCILLKL
                     KLSNGKLLVEGKTWTGFANSEEKYADDYVGKRIQPFWIEEEAGKIPNTNFVVGGRFRS
                     HAVRDGRLVTGQQQYSGAEAAKLVIEALGR"
     gene            1095..1463
                     /locus_tag="VEJ00_04290"
     CDS             1095..1463
                     /locus_tag="VEJ00_04290"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXX00409.1"
                     /translation="MKKVASLHDKALQSRDAVELLSDKWRIVVLHLLTGRPLRTSQLQ
                     RAIAGVSPKMLTQTLRGLERDGLVERHVGSVVPARVEYQLTSMGESVIPLLRDLCRWA
                     KAHRSMREAAQQRFNKQAAG"
     gene            complement(1493..>3581)
                     /locus_tag="VEJ00_04295"
     CDS             complement(1493..>3581)
                     /locus_tag="VEJ00_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXX00410.1"
                     /translation="TIVTTNTSGLPVGKIAEGFSDEFRWAWFGTHFFNPPRYMRLLEI
                     IPTPVADRAAIEAIAHFCDVRLGKGVVMAKDTPNFIGNRIGTFSVLNVMRLMQEMDLT
                     IEEVDALTGQAVGWPKSATFRTIDLVGLDILGAVVSNMTTNVHDERSDLRLPGFFAQM
                     LQRKWLGDKTKGGFYKKAKAADGKEDERLALDWKTLEYHPRQKPKFAALDMAKNIEES
                     GARLRTLLGLDGAGPQKGDKAGQFLWSALADLWTYAANRVPEISDSIVEIDRAMKLGF
                     NWELGPFELWDAAGVEATVARMKKEGKSVSPNVERLLSRGQKSWYIDDPKSTAGRKYW
                     DPGTESWEAIQVPQGVWSVTVAKKSNGVVKKNSGASLVDLGDGVACVEFHSKMNSLGA
                     DIISLILQTLKPGGPGDNFDAFVITNDATNFSVGANLMLLLMSVQEEEWDEVDMAIRQ
                     FQGMTQAIKFSPKPVVSAPFGLCLGGGTEISLHAAARQPHAELYTGLVEVGVGLLPGG
                     GGCKEMLLRAVDSASAARGKDSGDALARSVEMLQAMRHAFETIATAKVATSAHEARGL
                     GFLADSDRITMNRERVLSDAKVRALELFRAGYEPPVPRNDIPAPGENLLAALKMGVHL
                     MRQGGFVTDYEVKLGGKIAEVLCGGNVTPGTPVSEQYILDLEREGFKSLCGEKKTQER
                     IQYTLKTGKTLRN"
BASE COUNT          679 a         1138 c         1018 g          746 t
ORIGIN      
        1 cagccgaact tgccctctgc gcctgcctgg cacgtgtaga tctgtgatcc cgaagcgtgg
       61 gccataagca cgacttgcag gccggagggg gctgcgatcg cgtcgggcac gtcaggcgcg
      121 acctcggttt gggcagaagc agaaaggccc gccgggatca agatgcatag tgccatcacc
      181 ctggcaggcc aaaatctttg catgacacca tgaattctag caggtacggc tagcggccca
      241 gcgcctctat caccagcttc gcggcttctg cgccggagta ctgctgttgc ccggtgacga
      301 gccggccgtc gcggactgcg tgagatcgga agcggccgcc cacgacgaag ttcgtgttcg
      361 gaatctttcc cgcttcctcc tcgatccaga acggctggat gcgcttgccg acatagtcgt
      421 ccgcgtattt ctcctcggag ttggcaaagc ccgtccaggt cttgccttcc accagcagct
      481 ttccattgct cagcttcagt ttgagcagga tgcaggttgc gtggcaaatc accgccagaa
      541 ctttgccact ttcgtagaac tgcatcgcca gcttgtgcac ccgttcgtcg tcgaagaacg
      601 tgtacatcgg accctgcccg cccacaaaca ggactgcgtc aaaatcctga accttcagat
      661 ccttcatcgg cttggactgc tcgatctgcc tagcggtcgc cggcgcatgg atgaagccca
      721 tactgatcag atcgtcggcc gagtattggc tgtcgtcgcg cggatcgctc cacttgtcgc
      781 cttcgagccg cccgccgtcg gggctgaaaa tctcaacctg atagccgtgt tgggtaaact
      841 cccagtaggg atgcgtaact tcgccccacc agaagccgat gggccatccc gtctgcttgg
      901 atgtggctgg attcgaaacg ataattgcaa ctcgcttggg gtgagtggga tcgagaacat
      961 cgatttctac gctcatgcgt ctcctcggga aagattctgg gaacaagtct tggcaggggt
     1021 aggatgtatc tttatcagct cgacgcaacc gcttgcaacg cactcccagg taagcacgtt
     1081 accgcgaggt accgatgaaa aaggtggcca gccttcacga caaggctttg cagtcgcgag
     1141 atgctgtgga attgttgagc gacaagtggc gcatcgtcgt tttgcatctg ctgacaggca
     1201 ggccacttcg tacaagccag ttgcagcggg caatcgctgg cgtgtctccg aaaatgttga
     1261 cgcagactct gcggggattg gagcgcgacg ggctggtcga acgccatgtg ggaagcgtgg
     1321 tcccggcgcg tgtggaatac caactcacca gcatgggaga aagcgtaatc ccgctgctgc
     1381 gcgatttatg ccgctgggcc aaggcccaca ggagcatgcg cgaggccgcg cagcagcgct
     1441 tcaacaagca agccgcggga tgatccgagg gtcgctccac tcagaccaga attcagttcc
     1501 gcagcgtctt tcccgtcttc agcgtgtact gaatcctttc ctgcgttttt ttctcgccgc
     1561 acagcgattt gaagccttcg cgctccaggt cgaggatgta ctgttcgctc acgggcgtgc
     1621 cgggagtcac gttgcctcca cacagaacct ccgcaatctt ccctcccagc ttcacttcat
     1681 agtcggtgac gaaaccgccc tgccgcatga gatgaacacc catcttgagc gcagccagca
     1741 ggttctctcc gggagcggga atatcattgc gcggcaccgg cggttcgtat cccgcacgaa
     1801 acagttcaag cgcccgcacc ttggcatcgc tgagcacgcg ctcgcggttc atggtgatgc
     1861 ggtcggagtc cgcaagaaac cccagcccgc gcgcctcgtg tgccgaagtc gcgacttttg
     1921 ccgtcgcgat ggtctcgaac gcatgcctca tcgcttgcag catctcgacc gacctcgcca
     1981 gagcgtcgcc ggagtccttg ccccgcgccg ccgaagcgct gtccacggcg cgcagcagca
     2041 tttctttgca accgccgccg cccgggagca gtccaactcc cacttcgacc aacccggtgt
     2101 acagttccgc gtgcggctgg cgggccgcgg catgcagcga gatctccgtg ccgccgccca
     2161 ggcacagccc aaacggcgcc gaaacgacag gcttgggcga aaacttgatc gcctgcgtca
     2221 tcccctggaa ctgccggatc gccatatcca cctcatccca ttcttcttcc tgcacgctca
     2281 tgagcagcag catgaggttg gccccgaccg agaagttcgt cgcgtcgttg gtgatgacga
     2341 acgcgtcaaa gttgtctccg ggaccgcccg gcttcagcgt ttgcagaatc aggctgatga
     2401 tgtcggcgcc cagggaattc atcttcgagt gaaactcgac gcaggctacc ccatctccca
     2461 agtcaaccag cgaggcgccg gaattctttt tcacgacgcc attcgatttc ttcgccaccg
     2521 tcaccgacca gactccctgc ggaacctgaa tcgcttccca gctctccgtt cccggatccc
     2581 agtacttgcg gccggcagtc gatttcgggt cgtcgatgta ccacgacttt tgtccccgcg
     2641 acagcagccg ctcgacattc ggcgacacag acttgccttc tttcttcatg cgcgccacgg
     2701 tggcttcaac cccggccgca tcccatagtt cgaatgggcc aagttcccag ttgaacccca
     2761 gcttcatcgc gcgatcgatt tcaacgatgg agtcagaaat ctcgggcact cggttggcag
     2821 cgtaagtcca gagatccgcc agcgccgacc acaagaactg tcccgccttg tcgcctttct
     2881 gcggcccggc gccatccaga cccagcaagg tgcgcaggcg ggcgccggac tcttcgatgt
     2941 tcttggccat gtcgagcgct gcgaatttcg gcttctgccg cggatgatat tcgagcgtct
     3001 tccagtcgag agccagtcgc tcgtcttcct tgccgtcggc ggctttcgcc ttcttgtaga
     3061 agccgccttt ggttttgtcg cccagccact tgcgctgcag catttgcgcg aagaagccgg
     3121 ggaggcgcag atcgctgcgc tcgtcatgaa cgttagtcgt catgttgctg accacagcac
     3181 cgagaatgtc gagtccgacc aggtcaatgg tgcggaacgt ggccgacttc ggccagccca
     3241 cggcttgacc ggttagcgca tcgacttcct cgatggtcaa atccatttcc tgcatcagcc
     3301 gcatcacgtt caggaccgag aacgtcccaa tgcggttgcc gatgaagttg ggcgtatctt
     3361 tggccatcac cacgccctta cccagtcgca catcgcaaaa atgcgcgatg gcttcaatcg
     3421 ccgctcgatc ggccacgggc gtgggaatga tttccagcag gcgcatgtag cgtggcggat
     3481 tgaagaagtg cgtgccgaac caggcccagc gaaactcgtc tgagaacccc tcggcgatct
     3541 ttcccacggg aagcccgctg gtgttggtgg tgacgatggt t
//
LOCUS       DASPUL010000181         4511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_851677, whole genome shotgun sequence.
ACCESSION   DASPUL010000181 DASPUL010000000
VERSION     DASPUL010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851677"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VEJ00_04300"
     CDS             complement(<1..209)
                     /locus_tag="VEJ00_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00411.1"
                     /translation="MTLNSALQDLKQTTLSAISGMLGKLAYLASLRRAQGRYAHWGME
                     TVHGQESSHRAIKTAHVEVLACVLRT"
     gene            774..1313
                     /locus_tag="VEJ00_04305"
     CDS             774..1313
                     /locus_tag="VEJ00_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00412.1"
                     /translation="MLKFTLIAEIFSKVFDAYASLAKLGRISIRTVGVLLVLVAAWAA
                     AYSSEEGILGIIAGAHVLEQTIYLIECGLLVFIVLFSAYFHLAFSRPVFGIALGLGIS
                     ACVHLATWAVMANGGLSESPEKRALLDLVNMATYHVCVLIWFYYLLVPHKPANKPPVA
                     LPENNLDIWNRELERLLQR"
     gene            1310..1846
                     /locus_tag="VEJ00_04310"
     CDS             1310..1846
                     /locus_tag="VEJ00_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00413.1"
                     /translation="MNIAIILVVAAALALGIILRAAVTRRLTVSPQANLAAQIQPIDL
                     EAFRNLASPAEDDYLRRRLPPAAFRAVRRERLRAVAAYVHAAARNAEILVRIGQGALL
                     SSDARTVEAARQLVDQALLLRRNATFVLLRIYLALAWPYSGLGTTPILEDYVQLSGSA
                     MLLGRLQSPGSPVRISAS"
     gene            2095..>4511
                     /locus_tag="VEJ00_04315"
     CDS             2095..>4511
                     /locus_tag="VEJ00_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXX00414.1"
                     /translation="MLRISRSASVCFFLLLSTLAFGQGAATGDLHVTLKDPNGNAIAN
                     ATVTVRDVAKALERSATGDGQGGYSAQLLPPGTYSVTVEAAGFNKTEETGVGITVGGL
                     LELPISLSIATGKEIVEVSGQGELVETSRSSTTDTIGQRRIDNLPINGRDYINFTLTD
                     SQVVHDAAPNLGAAPTSGLNMSGQRARSNLVNVDGTDATDNSVNGVRSTVSQEAVQEF
                     QIITNNYAAEYGRASGGVVNIITRSGSNDFHGDVYGYLRNRNFQAVNPFSTVSNPAYT
                     RVQAGAAFGGAIKKDKTFYYFSYEITRRHETGFSSIGANNFELVNFDATNIFNPLAPA
                     GTFVMPATPQQVTFLTPFTQAGPAPQPVQLYTWLVGASTYLGLTGNQQSFAALGAVFA
                     PPGSGAQQFPTTCNVAVPFAPCAGLPTSFLSLKSQTGNFPVFEGTSLYSLRLDHNLTS
                     SNRLMLRANVSPSTVTGIEVSGQDQPFGQNAFSRTSEQTYRDVAGTVQDTWSIGNTKI
                     NEFRFQYARRGLSFFYNTQTGSGQAPAVNIPGFGYFGHEPYSYIQRTETRYQFTDNFS
                     WVIGRHNTKLGADLNVLPLDAIFTVNYGGVFDFGSFSLAPFGFPSSFPTLSAVQAYGA
                     GLPGDFIQGVGAPHDSFNNIPIGVFWQDSWRANKNITLNYGVRYDIEIPPKFKPITPA
                     AQAGYTVLGLQKGIQTQKDRVQPRVGIAWDPKGNGKTVLRSSYGIFYDHPLLALYFLG
                     DASDASSSVQLAFGPGLPCSGAGQPGDLNSFAIFQGVPLGVPGNPCSPATSAATATAM
                     DYSPSQQR"
BASE COUNT          849 a         1430 c         1250 g          982 t
ORIGIN      
        1 gtccgcagca cgcatgccag gacctcgaca tgcgctgtct tgatcgcgcg gtgggacgat
       61 tcctgcccgt gaaccgtctc catcccccaa tgcgcgtagc gtccctgcgc acggcgaagt
      121 gacgcaagat aggccaactt ccccagcatt cctgagatgg ccgacagcgt cgtttgcttc
      181 aagtcctgca gggctgaatt taaagtcatt ttgttacgaa ttccgaacgt tcctgcgatt
      241 ccgggcgtgc ttccacgagt ggttgggccg tgaaatcatc acgatccaat tttgcaatat
      301 caaggaggca acacccgtga aacgtatgct actcgtcctc gctgcagccg tagttctcct
      361 caacactttg gccatcccca ccatcgcaca cgccgacgga ggggcaggtg gtaccaattg
      421 tggcggaaaa atgtgcaaac cgtaacttca gcggcgcggt tcttacaaaa gccgaacaaa
      481 gatcccgagt gagttgccgc ctgcagcctg gcgcggtata gtgcgcgaga atcgccatgt
      541 tgcaggcgca ctcacttctt tggcactacc tttgggcggc accgaatatc ctgttgctcc
      601 tcctcgcttt tttgctctgg cgccgccact tgcatcgagc ttaccctttt ttcttcgcct
      661 tcgctttgct tggcgccacc gctcaactca cggtatatat caccgatgta ctccctttca
      721 ttgaaggacc aacctggtgg cgtatcttct gggcagctgt gcttgttgaa ggcgtgctga
      781 aatttacatt gattgcggag atcttttcca aggtctttga cgcctatgcg tcactggcga
      841 agttggggag gatctcgatc cgcacagtgg gagtgcttct ggtgctggta gctgcctggg
      901 ccgcagcgta ctcctcggag gagggaatcc ttgggatcat cgcgggagcg cacgtgctgg
      961 agcagacgat ttatctgatc gagtgcggac ttctcgtctt tatcgtcctt ttctcggcct
     1021 atttccacct cgccttcagc cgaccggtgt ttgggatcgc gctggggttg gggatatcgg
     1081 cctgtgtcca cctggctaca tgggccgtta tggcgaatgg agggctctca gagagtcctg
     1141 agaagcgtgc cctgcttgat ctcgtcaaca tggccaccta tcacgtctgc gtcttgatct
     1201 ggttctatta cctgctcgtt ccccacaaac ctgcaaacaa gcccccggtt gcattgccag
     1261 agaacaatct cgatatttgg aatcgcgaac tggagcgtct tctgcaaaga tgaatatcgc
     1321 cattattctc gtagtcgccg cagccctggc cttgggaatc attctccggg ccgcagtcac
     1381 cagaagattg acggtttctc cgcaggccaa cctggcggcg cagattcagc ccattgacct
     1441 cgaggctttc cgcaatctgg ccagtccggc tgaggacgac tacctgcgcc gtcgcttgcc
     1501 ccctgcggca ttccgcgcgg tgcggaggga acggctgcgc gccgtggctg cctacgttca
     1561 cgcggcggcg cgaaatgcgg agattctggt gcgcatcggg caaggtgcac tgctttcaag
     1621 cgatgcccgc accgtggaag ccgcccgcca actggtcgac caggcactcc tcctgcggcg
     1681 caacgcaacc ttcgtgctgc tccgcattta cctggccctg gcgtggccct attcgggctt
     1741 gggcacaacg cccatcctcg aagactatgt gcagctcagc ggctccgcca tgcttctcgg
     1801 gcggctgcag agtccggggt cgccggtacg tatctccgcc tcgtgagtga ttcggatcac
     1861 agacacttgc cgggggttct gccattctgc ccggccaggg cgcccgggtc gtgttcaagt
     1921 gcagttaact tggaaacgac cctcgtcgat tcctgacttg ttttcaggga ccaatctgtt
     1981 ataaaacctg cgggaatcag ctgcgcccaa gattaccccg tgaaaattgc gccatctgca
     2041 atgggctttg ctttcgccgt ctcctcgcct cggcgagttc gaagggagaa ccctatgctc
     2101 cgcatttcga gatccgcctc ggtgtgtttc ttcctgctgc tgtcaaccct ggcctttggg
     2161 caaggggctg ccaccggcga tctgcacgtc acgctgaaag atcccaacgg taatgccatt
     2221 gccaacgcca ccgtcacggt gcgcgacgtg gcaaaagctc tggagcgctc ggccacgggg
     2281 gatggccagg gcggctacag cgctcaactg ttgcctccgg gcacgtactc ggtcacggtc
     2341 gaagctgccg gattcaacaa gacggaagaa accggagtcg gcatcacggt cggtggcctg
     2401 ttggagctgc caatttccct ttcgatcgcg actggcaaag agatcgtcga agtcagtggg
     2461 cagggagaat tggtcgagac ctcgcgcagt tcgacgacag acacgattgg ccagcgccgc
     2521 atcgataatc tccccattaa cggccgcgac tatatcaact tcacgctgac cgattcgcag
     2581 gtggtgcatg acgctgctcc gaacctgggc gccgcaccga cctccggcct caacatgagc
     2641 ggccagcgcg cccggtccaa tctggtgaac gtcgacggaa cggacgccac cgacaattcg
     2701 gtgaacggcg tgcgctccac cgtctcgcag gaagcagtgc aggaattcca gatcatcacc
     2761 aacaactacg cggctgagta tgggcgggct tctggtggcg tggtgaacat catcacccgc
     2821 tcgggatcga acgactttca cggcgacgtc tacggctatc tgcgcaaccg aaacttccag
     2881 gcggtcaacc cgttcagcac agtttccaat cccgcttata cgcgcgtgca ggcgggagca
     2941 gcgtttggtg gcgccatcaa gaaagacaag acgttttact acttctcgta cgagatcacg
     3001 cggcggcacg agacgggatt ctccagcatc ggggcgaaca acttcgagct ggtgaacttc
     3061 gacgccacaa atatcttcaa tcctcttgcg cccgcgggga cgttcgtaat gccggcaact
     3121 cctcagcagg taactttcct cacaccgttt acacaagctg gtccagcgcc ccaacctgtt
     3181 caactttaca cgtggctagt gggtgcctcg acgtatctag gcctgaccgg aaaccagcag
     3241 tcgtttgccg cgttgggcgc tgtgttcgcg ccgcccggat ccggcgctca gcaattcccg
     3301 acaacatgca acgtggcggt tccctttgcc ccctgcgcgg gcttgcctac ctcctttttg
     3361 agtctcaagt cgcaaaccgg gaactttccc gtgtttgaag ggaccagcct ctactcgctg
     3421 cgtcttgacc acaacctcac gagcagcaac cgcctgatgc tgcgcgctaa cgtcagtccc
     3481 agcacggtaa ccggaatcga ggtcagcggg caggaccagc ccttcgggca gaatgcgttc
     3541 tcccgcacct ccgagcagac ttaccgcgat gtggcgggca cggtgcagga tacctggtca
     3601 atcggcaaca ccaaaatcaa tgaattccgc ttccagtacg ctcgccgcgg cctgtcattc
     3661 ttctacaaca cgcaaaccgg cagtgggcag gctccggccg tcaatatccc gggctttggc
     3721 tattttggcc acgagccgta ctcctacatt cagcgcaccg agacacgcta ccagttcacc
     3781 gacaattttt cctgggtgat cggccgccac aacaccaagc tcggtgccga cctcaacgtt
     3841 ttacctcttg acgccatctt cacggtcaac tacggcggtg tcttcgactt tggcagcttc
     3901 agcctggcgc ccttcggatt ccccagctca ttccccactc tttccgcggt gcaggcgtat
     3961 ggggccggcc tgcccggcga cttcatccag ggcgtgggcg ccccgcacga ttcgttcaac
     4021 aacattccca tcggcgtctt ctggcaggac tcctggcggg ccaataagaa cattactttg
     4081 aactacggtg tccgctacga catcgagatt ccgcccaaat tcaagccgat cactcctgcg
     4141 gctcaggccg gatacacggt gcttgggctg cagaaaggca tccagacgca gaaggatcgc
     4201 gtgcaacccc gcgtcggcat cgcctgggat cccaagggta acggtaagac cgtgctgcgc
     4261 agctcttacg gcatcttcta cgatcatcct ttgcttgcgc tgtatttcct gggggacgcc
     4321 tccgacgctt ccagcagcgt gcagctggca tttgggcccg gtttgccgtg ctccggggcg
     4381 ggccaacccg gcgatttgaa ctccttcgca atcttccagg gcgtgccgct cggtgtcccc
     4441 ggcaatccgt gctcgcccgc gacctctgcg gccacggcga ccgccatgga ctactcgccg
     4501 tcccagcaac g
//
LOCUS       DASPUL010000182         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1536205, whole genome shotgun sequence.
ACCESSION   DASPUL010000182 DASPUL010000000
VERSION     DASPUL010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536205"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VEJ00_04320"
     CDS             <1..1029
                     /locus_tag="VEJ00_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXX00415.1"
                     /translation="LPYEPVMKVVAGRTEAKVKEAAERLGWEEYATRWEDVIARKDID
                     IIDICTPGDSHMPIAIAAAQAKKHILCEKPLANTLGDSEKMWKAAAAAGVIHMLCHNY
                     RRCPAVALAKQLIDAGTLGKIYHYRGTYLQDWIVDPAFPRVWRLEKSRAGSGSLGDIL
                     SHTMDLSRYLVGEPVEVNGLLKTFITERPLPDNPAKKGPVDVDDAALSLVRYDNGAVG
                     YLEGTRFATGRKNYNRFEINGAKGSIVWDLEQLNDLQLYVEEGPNSGFRTITVTDGKH
                     PYVGAWWPPGHIIGYEHSFTHTVYDFLRAIGEQKLPAPSFEDGYKNQRVLDAIERSAR
                     EGKWMKIG"
     gene            complement(1183..1677)
                     /locus_tag="VEJ00_04325"
     CDS             complement(1183..1677)
                     /locus_tag="VEJ00_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00416.1"
                     /translation="MKKCQVIGSTVSLVFAACSLAVNAAPMKELDAYKASLGKWACVV
                     KDLGSGKTFKSVAEKTAEFDGNTYVERYYEIASADHPDAWKAIFIMSYDPQTNRWVRS
                     GVDNSGGRNAASSSGWNGDTWVWENDAVNIVITQKGAKAYMFGVDVKEGSGVKRVAEG
                     SCTR"
BASE COUNT          416 a          630 c          602 g          382 t
ORIGIN      
        1 cttccgtacg aaccggtgat gaaagttgtg gccggccgca cggaagcgaa ggtcaaagag
       61 gccgccgagc gtctgggctg ggaggagtac gcgacgcgct gggaggatgt catcgcgcgc
      121 aaggacatcg acatcatcga catctgcacg cccggcgatt cgcacatgcc gatcgccata
      181 gcggccgcgc aggcaaagaa acacatcttg tgcgaaaagc cgctcgccaa cacgctgggc
      241 gattccgaga agatgtggaa agccgcagcc gcggccggcg tgatccacat gctgtgccac
      301 aactaccggc gctgtcccgc ggtcgcgcta gccaagcagc tgatcgacgc gggcacgctc
      361 ggaaaaatct accactaccg cggcacctac ttgcaggact ggatcgtcga tccggcgttc
      421 ccgcgggtgt ggaggctgga gaaatccagg gcgggttcgg gatcgctggg cgatatcctc
      481 tcgcacacga tggatctgtc gcgctatctc gtcggcgaac ccgtcgaggt caacggcctg
      541 ctcaagactt tcatcaccga gcggcctctg cccgacaatc cggcgaagaa aggcccggtc
      601 gatgtcgacg acgcagcgct atcgctagtt cgctacgaca atggcgcggt cggctacctg
      661 gaggggacgc gcttcgcgac cggccgcaag aactacaacc gcttcgagat caacggcgcc
      721 aaaggcagca tcgtgtggga cctcgagcag ttgaacgacc tgcaactcta cgtggaagaa
      781 gggcccaaca gcggctttcg cacgatcacc gtcaccgacg gcaagcaccc ctacgttggg
      841 gcgtggtggc cgccggggca catcatcggc tacgagcatt cgttcacgca caccgtctac
      901 gattttctgc gcgcgatcgg tgagcagaag ctgcccgcac ccagcttcga ggacggctac
      961 aagaaccagc gggtgctcga cgccatcgaa cggtcggcac gggaagggaa gtggatgaag
     1021 atcggatagc ggaacggttg cgcgcggttt ctgaatacag gcactgaact ggcgcgttcg
     1081 atatgccgca ccgatagatg gtttcggcgg caactgtgcc gccgaaaccg ggagagcagg
     1141 cgctctacgg tcggatcgtg ccggccgcag ggcgaggcgc agctagcgcg tgcacgaacc
     1201 ttctgccact ctcttgaccc cagagccttc cttcacgtcg acgccaaaca tatacgcctt
     1261 ggcacctttt tgcgtgatca cgatgttcac cgcatcgttc tcccacaccc aggtatcgcc
     1321 gttccagccg ctcgagctgg ccgcgttgcg cccgcccgaa ttatccaccc cgctgcgtac
     1381 ccagcgattg gtctgtgggt cgtacgacat gatgaagatc gccttccacg cgtccgggtg
     1441 gtcggcactg gcgatctcat agtagcgttc cacatacgta ttgccgtcaa attcggcggt
     1501 cttctccgcc accgacttga aggtctttcc ggaacccaga tctttcacga cacaagccca
     1561 cttgccgagg ctcgctttat aggcatccaa ctccttcatg ggcgctgcgt tgacagcgag
     1621 cgagcaagct gcgaatacga gggatacggt cgagccgatt acctgacatt tcttcatttt
     1681 ggattctcct ttttctcgga cgtaacggac atctagacca gcacgaactt gcgggatcgc
     1741 acagcgcatc atccccatcg gctctgtggt tcgcccccgc aatcgggatt ggaatcttcg
     1801 gtgccttgcc gcgatgcctg tgcgaatccc acgatggtcc ggctcgtcgc aaagtttaga
     1861 gtggagcgca ctcggctggt ataacccaaa tgggctatgc ggcatctata acatggaatc
     1921 cgggaactcg atcagctgct ctcccgtaac agcccggcat gtcggaagtg tcattgcatc
     1981 tgccgccagt gtccagacgt cgagcgcatg gtaatactgc gcgtaaggca
//
LOCUS       DASPUL010000183         3748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_168147, whole genome shotgun sequence.
ACCESSION   DASPUL010000183 DASPUL010000000
VERSION     DASPUL010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168147"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VEJ00_04330"
     CDS             <1..1152
                     /locus_tag="VEJ00_04330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXX00417.1"
                     /translation="VLEGETIGWGASSRNGGMVLTGMKLGVNKLISMYGRERTQRMYA
                     ASLASIDCVEQIVREEAIACDFSRCGHLEVACKQKHFDDYARQAETIAREFNHTLRVV
                     QKNELSAEIGSNIYFGGMVDETSAGCNPARYVAGLARASVKAGAQVFEHARVQTIERG
                     SRNGDPGWKLSTSRGPLWAHEVFVGTSGYTGAATPSLQKKIIPIGSFIITTEILPEKL
                     AHELSPRNRMIYDSKNYLYYYRLTPDHRMLFGGRAAFFPENAQTIRRSAEILRRGMIG
                     VYPQLDDAKIDYVWGGTLDFAFDIMPHAGQLDGMYYAVGYAGHGVAMATYQGQKIAEL
                     MASERSGGAKPENPFVSIPFPGAPLGLYNGKPWFLPFAGAWYKFLDWVS"
     gene            complement(1254..1664)
                     /locus_tag="VEJ00_04335"
     CDS             complement(1254..1664)
                     /locus_tag="VEJ00_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXX00418.1"
                     /translation="MPMRTKAFFDTNVLIYAVAENDPRAARSEELLANGGVISVQVLN
                     EFTSVARRKLDMSWDEVREALTAIRILCPDAVAVTVATLDSGLKIAEDYGYGIYDALI
                     AASALEAKCATLYSEDLQNGQVIEGSLTVRNPFV"
     gene            complement(1648..1878)
                     /locus_tag="VEJ00_04340"
     CDS             complement(1648..1878)
                     /locus_tag="VEJ00_04340"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXX00419.1"
                     /translation="MQVAKWGNSLAVRLPATVVEALELQEGDQIEIRIAQGRVFEVSR
                     DRSRERALARLRELRRPLPAGFVFERDDADAH"
     gene            complement(2166..3527)
                     /locus_tag="VEJ00_04345"
     CDS             complement(2166..3527)
                     /locus_tag="VEJ00_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXX00420.1"
                     /translation="MNSNRIPSTSKPSALRKAGLFYLVFVMFSYTTGGPFGLEDMVTT
                     SGPGITLIYLLVLPFFWCIPVSLVSAELTTAMPVEGGFYRWTRAAFGDFWGFLAGWWN
                     WSASFLLGGIYAVLFTDYVVYYLPRMTWWEHYFISVSLIVVLTWVNVRGIQAVGRVAT
                     ALEIFIFVPVATMIVIGFMHWHYNPFTPWVVPNQPTFKVFGVGLAVGLWLYSGYEQLS
                     TVAEEVENPQRAYPRALALVVPLSMAAYFLPALAGLASAGEWEKWHSGFFSDAAAIIG
                     GTFHGPGWLSKSWLGTWMALAAMVASVALLNSTILTTTRMPFAMAQDGYLPSALTRKH
                     ARYGTPWLAIVASAAIYALLAWKSLAQLISVYIWLRSATTVLTVLSAWRLRKTRPDLK
                     RSFTIPGGRAGLLYVVGAPVVMALVALLGSDRFGLIGGAIGIVLGPVVYGVVKGKKAV
                     VGR"
BASE COUNT          803 a         1242 c         1065 g          638 t
ORIGIN      
        1 gtgctcgaag gggagaccat cggctggggc gccagctcgc gcaacggagg catggtcctc
       61 accggcatga agctcggcgt gaacaagctc atctccatgt acgggcgcga gcgcacgcag
      121 cgcatgtacg ccgcatcgct ggcttcgatc gattgcgtcg aacagatcgt ccgcgaagaa
      181 gccatcgcct gcgacttctc ccgctgcggc catctcgaag tcgcctgcaa gcagaagcat
      241 ttcgacgact atgcccgcca ggccgagacc atcgcccgcg aattcaacca caccctgcgc
      301 gtggtgcaga agaacgagct gagcgcggag atcggctcga acatttattt cggtggcatg
      361 gtcgatgaaa ccagcgccgg gtgcaatccc gcgcgctacg ttgccgggct ggcccgcgcc
      421 tccgtgaagg ccggggcaca ggttttcgag cacgctcgcg tacagaccat cgagcgcggt
      481 tcacgcaatg gagatccggg ctggaagctt tccacttcgc gcggcccgct ctgggcgcac
      541 gaagttttcg tcggcaccag cggatacacg ggcgcggcta cgccctcgct gcagaaaaag
      601 atcattccca tcggctcgtt catcatcacg accgaaattc tgccggaaaa gctggcgcac
      661 gagctcagcc cgcgcaaccg tatgatctac gattcgaaga actatctcta ctactaccgg
      721 ctcacgcccg accaccgcat gctgttcggc ggccgcgccg cgttcttccc tgaaaacgcg
      781 cagaccattc gccgcagcgc cgaaatcctg cgccgcggca tgatcggcgt ctatccccag
      841 ctggacgacg ccaaaatcga ctacgtctgg ggaggcacgc tcgacttcgc cttcgacatc
      901 atgccgcacg ccggccagct cgacggcatg tactacgctg tcggttatgc aggacacggc
      961 gtggccatgg cgacgtacca gggccagaaa atcgcagagc tcatggcgtc ggagcggtca
     1021 ggaggcgcca agccagagaa tcctttcgtt agcatcccct tccccggcgc accgctcggc
     1081 ctctacaacg gcaagccctg gttcctgccc ttcgccgggg cgtggtacaa gttcctcgat
     1141 tgggtgagtt gaggttgggt gtcgaagggc gtgagcgcgg aagaggggca cttcagcgcc
     1201 gtctaagtcg ccttaatttc aaaatgggct tcagccctgg cctagtcgcg agatcacaca
     1261 aacggattcc tcaccgtcag gctgccctca atgacctgcc cgttttgcaa gtcttccgaa
     1321 tacagcgttg cgcactttgc ttccagggct gaggccgcga tcagcgcgtc gtagattccg
     1381 tagccataat cttcggcaat cttcagcccg ctgtcgaggg ttgccacggt caccgcaacg
     1441 gcatcagggc aaagaattcg gatcgccgtg agcgcttccc tcacctcatc ccacgacata
     1501 tccagcttgc gccgagcgac cgaggtgaac tcgttcagca cttgcacgct gatcacgccc
     1561 ccgttcgcca gcaactcttc tgaccgcgcc gcgcgcggat cattctccgc cacggcataa
     1621 atcaaaacgt tcgtatcgaa gaacgcctta gtgcgcatcg gcatcgtcgc gctcgaacac
     1681 gaaaccggcg ggcagcggac gccgcagctc gcgcagccgc gccagcgccc gttcgcgact
     1741 acggtcgcgg ctcacttcaa acacccgccc ttgcgcaatg cgaatctcga tctgatcccc
     1801 ttcctgcagc tcgagcgcct cgaccacggt ggcaggcagt cgcactgcca gactgtttcc
     1861 ccacttcgcg acctgcatga ctaagcctct tggatataca tcataattgt atatcttttg
     1921 aagtcataaa acaactggct tgatgccaac gcatcaagga cggtgcaggc cctcccaaga
     1981 aatagttttc agggtactta agggcggact gggttgtgga aaatgattcc cataacctgt
     2041 tgaaaacagg gcacataaat ccggcagcta aatcgattta gctggccgat cagccagccc
     2101 gtgcactgcc gcgcgaggca tcccacgaag cggcctctgt cgaagcgtcc ccacacgacc
     2161 gttgcctacc gaccaacaac cgctttcttc cccttcacca ccccatacac caccggcccc
     2221 aagacgatcc cgatcgcgcc tccgatcagg ccgaagcggt cgctgcccag cagagccacc
     2281 agcgccatca ccaccggcgc gccaaccaca taaagcaatc ccgcgcgtcc gccggggatg
     2341 gtgaacgagc gcttcagatc cgggcgcgtt tttcgcagtc tccacgccga gagcacagtc
     2401 agcaccgtcg tcgccgagcg cagccagatg taaaccgaaa tcagctgcgc cagactcttc
     2461 caggcgagca gcgcgtagat cgccgccgac gccacgatcg ccagccacgg agtcccgtag
     2521 cgcgcgtgct tgcgggtaag cgcgctgggc agatatccgt cctgggccat ggcgaacggc
     2581 atgcgcgtgg tcgtgaggat tgtgctgttg agcaaggcca cgctcgccac catggcggcg
     2641 agcgccatcc atgtgcccag ccaggatttg ctgagccaac ctggaccgtg aaatgtccct
     2701 ccgatgatgg ccgcggcatc ggagaaaaaa cccgaatgcc acttctccca ctcgcccgcc
     2761 gacgccaggc cggccagtgc cggcagaaag tacgccgcca tcgacagcgg caccaccagc
     2821 gccaatgccc ggggatacgc tcgctgcgga ttctcgactt cttcggcaac ggtcgacagt
     2881 tgttcgtaac ctgagtacag ccacaacccc accgccagcc ccaccccgaa taccttgaac
     2941 gtcggctgat tgggcaccac ccagggcgtg aacgggttgt agtgccagtg catgaatccg
     3001 atgacgatca tggtcgccac cgggacgaaa atgaaaatct cgagcgcggt cgcgacgcgg
     3061 ccgaccgcct gaatgccgcg cacgttgacc caggtgagca ccacaatcag cgacacagaa
     3121 atgaagtagt gctcccacca cgtcatcctg gggaggtagt acaccacata atccgtgaac
     3181 agcacggcgt agatgccgcc cagaagaaac gatgccgacc agttccacca ccccgcgaga
     3241 aatccccaga agtcgccaaa ggcggcgcgc gtccagcgat agaagccgcc ctcgaccggc
     3301 atggccgtgg tcagctccgc cgaaaccagc gacaccggaa tgcaccagaa aaacggcagc
     3361 accagcaggt agatcagcgt gatccccggc cccgaagtcg tgaccatgtc ttcgagtccg
     3421 aatggcccgc ccgtggtgta agaaaacatc acgaagacga gatagaacag gccggccttg
     3481 cgcagcgcgg aaggcttgga ggtggacggg atgcggttcg agttcaaggc ggctttcgtg
     3541 gacggaattg tagacgatgg atcgagcctg tgctgaatcg atcagaatct gccgccaacc
     3601 ggagtcaagc ggcctgcggg cccgcacgtt caggctgctg ctccactctt gcgaacgagc
     3661 gggtcaggcg catggaattt cccacgactg acaaactgga aagcagcatg gcggatgccg
     3721 ccatgatcgg gttcaggact ccgatgac
//
LOCUS       DASPUL010000184         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1399773, whole genome shotgun sequence.
ACCESSION   DASPUL010000184 DASPUL010000000
VERSION     DASPUL010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399773"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VEJ00_04350"
     CDS             <1..250
                     /locus_tag="VEJ00_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00421.1"
                     /translation="TDAWFVGFSPTITCGVWVGYDEKKSLGAKETGAHAALPIWMNFM
                     TAAMAGKDAGDFQSPPATEHVGQKVDTPDTAPATEESH"
     gene            complement(255..1040)
                     /locus_tag="VEJ00_04355"
     CDS             complement(255..1040)
                     /locus_tag="VEJ00_04355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX00422.1"
                     /translation="MTGAASGIGRAIAISLAQRGCDLVLADLNEEKLSETARMASVHC
                     VRISRHLVDVADRQAVAAFPQAVMAQHPGVDILVNNAGVAIGGTFDEASEEDFEWLFD
                     INFWGVVRMTRAFLPLLKQSDDARVVNLSSLYGLISPPGQTAYSASKFAVRGFSNALR
                     HELAGSHIGITVVHPGGVATSIADSARVPKNATPEDIAQRKARIKKALRMPPAVAGEI
                     IVKGIERRQSRIIVGTDAKVLSLIERLAPVSYWKVLTRLMPRA"
     gene            complement(1086..>2270)
                     /locus_tag="VEJ00_04360"
     CDS             complement(1086..>2270)
                     /locus_tag="VEJ00_04360"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXX00423.1"
                     /translation="IRFNRRVVRASWSSPDARWTIEATGPDGPVEFTCNFLYMCSGYY
                     EYESGYMPGWLGMDRFTGRTVHPQQWPEDLDYAGKRVVVIGSGATAVTLVPAMTDKAA
                     HVTMLQRSPTYIVTRPSEDAIANWLHRRLPGRLGHGLSRWKNIFLQMYFYGVARLRPE
                     ATRNAILRFAQLQLGPDYEVGKHFNPRYNPWDQRLCLVPDGDLFAAIRAGKASVVTDE
                     IETFTEGGIRLRSGTQLEADIVVTATGLKMRLMGGMQVFVDGVPVDFAKTMTYKGMMS
                     SDVPNLASALGYTNASWTLKCDLTSEYVCRLLNFMDHHGYASCTPRRRDPSITEEPAL
                     SFTSGYVQRALPHLPRQGSKKPWRLNQNYALDLMAMRFCKLDDGTLEFTPRSRKPTPV
                     RA"
BASE COUNT          421 a          651 c          778 g          420 t
ORIGIN      
        1 cacggacgcg tggtttgtgg gattctctcc taccattact tgcggggtat gggtcggcta
       61 cgacgaaaag aaatcgctgg gcgcgaaaga aacgggcgcg cacgcggccc tgcccatctg
      121 gatgaacttc atgaccgcgg cgatggcggg gaaagacgcg ggggatttcc agtcaccgcc
      181 ggcgacggag cacgtggggc agaaggtcga cactcccgat acggcgccgg cgacggaaga
      241 gtcgcactga cggtctacgc gcggggcatc agccgggtaa gaaccttcca gtatgagacc
      301 ggggcgaggc gctcgatcaa tgaaagaacc ttggcgtctg tgccaacgat gatgcgcgac
      361 tggcggcgct caatgccctt gacgatgatc tcgccggcga cggcaggcgg catgcgcagt
      421 gcttttttga tgcgcgcctt cctttgcgcg atatcttccg gcgttgcgtt cttcggcaca
      481 cgggcgctgt cggcgatgga ggtcgccact ccgccggggt gcaccacggt gataccgatg
      541 tgcgagccgg cgagctcatg ccgcaaagca ttcgagaagc cgcgcacggc gaacttgctt
      601 gccgaatagg ccgtctgccc cggtggcgag atcaggccgt aaaggctgga gagattgacc
      661 acgcgggcgt cgtcgctttg tttcagtagc ggcaggaaag cgcgagtcat ccgcaccacg
      721 ccccagaaat taatgtcgaa cagccactcg aagtcttctt cactggcctc gtcgaacgtg
      781 ccgccgatgg cgactccggc gttgttgacc aaaatgtcga ctcccgggtg ctgcgccatc
      841 accgcttgcg ggaaggccgc cacggcctga cggtcggcaa cgtcgacgag atgccggctg
      901 atgcggacgc agtggaccga agccatccgg gcggtctcag agagcttctc ttcgttcagg
      961 tcggcgagga caaggtcgca accgcgctgc gcaagcgaga tcgcgatcgc acgtccgatg
     1021 ccgctggcgg caccggtcac gacagcggtt cgtttggcga gtctcatcat gttcgacatg
     1081 ggtgactatg ccctgacggg cgtgggtttc cggctgcgag gcgtgaattc cagagtgccg
     1141 tcatccagct tgcagaaacg catagccatc agatcgagag cgtagttctg gttcaggcgc
     1201 cagggtttct tcgatccctg ccggggaaga tgcggcagcg cacgctgcac gtatcccgaa
     1261 gtgaagctca gcgcgggctc ttcggtgatg gagggatcgc gccggcgcgg tgtgcaggag
     1321 gcgtagccgt ggtggtccat gaaattcagg aggcggcaga catattccga agtgagatcg
     1381 cacttcagcg tccacgaggc gttcgtatag ccgagtgctg aggccaggtt agggacgtcg
     1441 ctgctcatca tgcccttgta ggtcatggtt ttcgcaaagt cgaccggcac tccgtcgacg
     1501 aagacctgca ttcctcccat caacctcatc ttcaggccgg tggcggtgac gactatgtcg
     1561 gcctcgagtt gtgtgccgga gcgcaggcgg atgccgccct cggtgaaggt ctcgatctcg
     1621 tcggtcacga ccgaggcttt gccggcgcgg atcgcggcga acagatcgcc atcgggaacg
     1681 aggcagaggc gctggtccca ggggttgtag cgggggttga agtgcttgcc gacttcgtaa
     1741 tcgggcccga gctggagttg cgcgaacctg aggatggcgt ttcttgtggc ctcaggtctg
     1801 aggcgcgcca ctccgtagaa gtacatctgc aggaagatgt tcttccagcg cgagagccca
     1861 tgcccgagcc ggccgggaag acggcggtgc agccagttgg caatggcgtc ttcggagggg
     1921 cgggtgacga tgtacgtagg cgagcgctgc agcatggtca cgtgcgcagc tttgtcagtc
     1981 atcgccggaa ccagcgttac agcggtggcg ccgctgccga tgaccacgac gcgcttgccc
     2041 gcgtaatcga gatcctcagg ccattgctgc ggatgcacgg tgcggccggt gaagcgatcc
     2101 atgccgagcc agcccggcat gtatccggac tcgtattcgt aatagccgct gcacatgtag
     2161 aggaagttgc aggtgaactc gactggtcca tcggggccgg tggcttcaat ggtccagcgg
     2221 gcatcgggcg aagaccagga ggcgcgcaca acgcggcggt tgaagcgaat
//
LOCUS       DASPUL010000185         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_715322, whole genome shotgun sequence.
ACCESSION   DASPUL010000185 DASPUL010000000
VERSION     DASPUL010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715322"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VEJ00_04365"
     CDS             complement(<1..938)
                     /locus_tag="VEJ00_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HXX00424.1"
                     /translation="MKNNHHGNSRNPNPGQTPGGEGKERHLHNPIEDRLTPLEPLDLS
                     KIRSIDDLVRAMARTAFTGRQLGEAADVLEAMAADEDCFVVMTLAGAMTVAKQGLIIT
                     ELIDRGIVNAIVSTGALMAHGLVEATGRAHFRANPEVSDEELYDQGYNRVYDTLEPEQ
                     NLDDVEEVMSAVLEQWDHNEVMCSYKLNHAIGAHLAKHAQGQRGILKSAYEKGVPVFV
                     PAFTDSELGLDTALNNRIRESTGRHKIRYDPFEDLEHFAATLLRQKRLGIFTIGGGVP
                     RNWSQQFGPFIELRHRRLGEKIPLKRYHYGLRICPEP"
     gene            1103..2005
                     /locus_tag="VEJ00_04370"
     CDS             1103..2005
                     /locus_tag="VEJ00_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HXX00425.1"
                     /translation="MAPEKFRVALVQMSCSPEPEQNLEKALGRIADAAGRSCQVVCLP
                     ELFQTQYFCQREDHALFDLAESIPGPTSEKLAAAARANRVVLIASLFEKRAPGVYHNT
                     AAIFDSDGSLRGLYRKMHIPDDPLYYEKFYFTPGDLGFRALDTSVGRLGTLVCWDQWY
                     PEGARLTALQGAQVLFYPTAIGWHPAEKAEFGVAQHDAWRTIQRAHAIANGVYVAVVN
                     RVGFETGNVRGKSAPGQGLEFWGGSFFCDPFGRVLAEASHDREEILIGDVDLKALEDI
                     RRNWPFLRDRRIDAYAPITNRLLD"
     gene            2096..3154
                     /locus_tag="VEJ00_04375"
     CDS             2096..3154
                     /locus_tag="VEJ00_04375"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HXX00426.1"
                     /translation="MPAEWEPHAATWLAWPHYHGDWPGRFEPIPWVYAEIFRNLAKHE
                     CVELIVNDRSAARQARRVLERADAWSKNIRFHFWPTNRVWLRDSGCIFLKRGAGTHAR
                     ETAPPSLALKFRFNAWAKYSNWRHDDRVGCLMGYTKSPGRRHAHEIRPVSNGKHIVLE
                     GGSIDVNGAGTILTTEECLLSKIQERNPHMTRVQYETAFRDYLGAPHTIWLGRGIVGD
                     DTHGHVDDLSRFVAKDTVVTMVEPNTKDPNHAPLLANLRRLQAARDQNGRKFTIVELP
                     MPQPVVFENRRLPASYANFYIANGVVLAPVFNRPNDRIALNTLAQLFPKRDIVPIYSG
                     DFIWGLGAMHCMTQQQPA"
BASE COUNT          594 a         1020 c          919 g          633 t
ORIGIN      
        1 ggctcggggc agatgcgcaa gccgtagtga tagcgcttga gcggaatctt ctctcccaac
       61 cgtcggtgcc tgagctcgat gaacggcccg aactgctgcg accagttccg cggtacgccg
      121 ccgccgatcg tgaagatgcc taaccttttc tgccgcagca gggtcgcggc aaagtgttcc
      181 agatcttcga agggatcgta gcggattttg tgccggccgg ttgactcccg gatgcggttg
      241 ttcagggcgg tgtccaagcc cagctcggag tcggtaaacg ccggcacgaa cacgggaacg
      301 cctttctcgt acgcggactt caggattccg cgctgtcctt gggcatgctt cgccaggtgc
      361 gctccgatcg cgtggttcag cttgtaggaa cacatcacct cgttgtggtc ccattgttcg
      421 agcaccgcgg acatgacttc ctcgacgtca tccagattct gctccggctc gagcgtgtca
      481 tagacccggt tgtagccctg atcgtagagt tcttcgtcgg aaacctcagg attggcgcgg
      541 aagtgtgcac ggccggtggc ctcgacgagc ccgtgcgcca tgagcgcgcc ggtggaaacg
      601 atggcattga cgattccgcg atcgatcaac tcggtgatga tcaatccctg cttcgcaact
      661 gtcatggcgc ccgccagggt catcacgacg aaacagtctt cgtcggcggc catcgcttcg
      721 agcacatccg ccgcctcacc caattggcgg ccggtaaacg cggtcctggc catcgctcga
      781 accagatcgt cgatcgagcg gatcttgctg agatcgagcg gctcgagcgg cgtgagccgg
      841 tcctcgatcg gattgtgcag atgacgctcc ttgccttcac cgcccggcgt ctgccctggg
      901 ttcggattgc gcgagttgcc atgatgattg ttcttcacag ctgcctccca gccctctagc
      961 ccaagaggga ttcgtcacga caacatactt tacaggaatg ccctcgattg ccgaaagctg
     1021 acgggtacct cgttttgatt tccggcatga cgcctcgacc acagtctctc gaatgctata
     1081 ttgttcgcgg aggccggaac gcgtggcacc tgaaaaattt cgtgtcgccc tggtgcagat
     1141 gtcgtgcagc ccggagccgg agcagaatct cgaaaaggct ctcggccgca tcgccgacgc
     1201 cgctggccgc tcctgtcaag tcgtctgcct tccggaactc tttcagaccc agtacttctg
     1261 ccagcgcgag gatcacgcgc ttttcgatct ggctgaatct attcccggcc cgacttccga
     1321 gaaactcgct gcagccgccc gtgccaacag ggtcgtgctg atcgcatcct tgttcgaaaa
     1381 gcgcgcgccc ggggtttacc acaacactgc ggcgattttc gacagcgacg gctccctgcg
     1441 cggcctctac cgcaagatgc acatcccgga cgatcccctg tactacgaga agttctattt
     1501 cactcccggg gatctcggct tccgcgcact cgacaccagc gtcggacgcc tgggcactct
     1561 cgtgtgctgg gaccagtggt atcccgaggg cgcccggctg acggcgctgc agggtgcgca
     1621 ggtgcttttc tatccgacgg cgatcggctg gcatccagcc gagaaagccg aattcggcgt
     1681 tgcccagcac gatgcgtggc gtaccatcca gcgtgcgcac gccatcgcca acggcgtcta
     1741 cgtggcggtt gtaaaccgcg tcggcttcga aaccggtaac gtccgcggca agtcagcccc
     1801 cggacaggga ctcgaattct ggggtgggtc attcttctgc gatcccttcg ggcgcgtgct
     1861 ggccgaggcg tcgcatgatc gagaggaaat tcttatcggc gacgtcgacc tcaaagcgct
     1921 ggaagacatt cgccgcaact ggccgttcct gcgggaccgc cgcatcgatg cctatgcgcc
     1981 gattaccaac cgactgctgg actaggacag attgaaagtg gctaaagccc cccgccgatc
     2041 ttcaagtaat acttcaagag atcatcacgt cactcctgcc actctcggct atcgcatgcc
     2101 ggccgagtgg gagcctcacg ccgccacctg gctggcgtgg ccccactacc acggggattg
     2161 gccgggacga tttgaaccca tcccgtgggt ctatgctgag atctttcgca atctggcaaa
     2221 acatgagtgc gtcgagttga tcgttaacga caggtccgcg gcgagacagg cgcgccgagt
     2281 cctggagcgg gccgatgcgt ggtcgaagaa tatccggttt catttttggc ccacgaatcg
     2341 cgtctggttg cgcgattccg gctgcatctt cctgaagcgt ggcgcgggca ctcatgcccg
     2401 cgaaacggcg ccgccgtcgc tggccctgaa gtttcgcttc aacgcctggg ccaaatattc
     2461 gaactggcgg catgacgaca gagtcggctg cctgatggga tacaccaaga gtcccggccg
     2521 tcggcatgca cacgagattc gccccgtttc caacggaaag cacatcgtcc tcgaaggcgg
     2581 ctcgatcgac gtcaatggcg ccggaaccat tctcacgacc gaagagtgcc tgctgagcaa
     2641 gatccaggaa cgcaatccgc acatgacccg cgtgcagtac gagaccgcct tccgcgacta
     2701 cctcggggcc ccgcacacga tctggctggg acgaggcatc gtcggcgacg acactcacgg
     2761 gcacgttgac gacctctctc gcttcgtggc aaaggacacg gtcgtcacca tggtcgaacc
     2821 caacacgaag gatcccaatc acgctcctct gctcgccaac ctgcgccgcc tgcaggccgc
     2881 acgcgaccag aacggccgca agttcaccat cgttgagttg cctatgccgc aaccggtggt
     2941 attcgaaaac cgccggctgc ccgccagcta cgccaacttc tacattgcca acggagttgt
     3001 gctcgctccg gttttcaatc gccccaatga ccgcattgcg ctcaacacgc tggcgcagct
     3061 ttttcccaag cgcgacatcg tccctattta ttcgggagac ttcatctggg gcctgggcgc
     3121 gatgcactgc atgacccagc agcaaccggc atgagccacc atgagg
//
LOCUS       DASPUL010000186         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_853586, whole genome shotgun sequence.
ACCESSION   DASPUL010000186 DASPUL010000000
VERSION     DASPUL010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853586"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VEJ00_04380"
     CDS             complement(<1..587)
                     /locus_tag="VEJ00_04380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine dimethyltransferase family protein"
                     /protein_id="HXX00427.1"
                     /translation="MPKVASARRPQAKPKLGQHFLASDDSAAGIVGALGDISQSTVLE
                     IGPGRGILTSLLARRARRLIAVEVDRVLAAQLRLKFGMARNVEILEADILTIDFDSLF
                     GPKPGLGRPGIDIKPEPVRVVGNLPYYITSDILLRLFEFAKYFDTIVIMVQREVADRI
                     AASPGGRDYGMLSATTQLYAHVEKLFTLPPEAFVPP"
     gene            719..2200
                     /gene="glgA"
                     /locus_tag="VEJ00_04385"
     CDS             719..2200
                     /gene="glgA"
                     /locus_tag="VEJ00_04385"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001903.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HXX00428.1"
                     /translation="MHVAFVASEGVPFSKTGGLADVVGALPRALAALGHQVSVYLPRY
                     RQTKLADPPTVVRSITIPFDDKYRFASIVGGVNQGGVRFYFVEYPKYFDRDALYGTPA
                     GDYPDNAERFALFSRAVLEASKILGVPQIFHCHDWQSALVPVLLRTVYAEDPAFREAG
                     TVFTIHNMGYQGLFPPDTLPLLMLPWDLFTMSKMEFFGQVNFLKGALTYSDFITTVSR
                     KYSQEIQTAEYGFGLEGVLRDRAATVTGILNGVDYDEWSPQTDKFTAATYSPQDLSGK
                     AKDKQDLLAAFGATKADPKLPVIGIVSRFAAQKGFDLIAQIADRLAREEMTIVALGAG
                     DKAYEELFLRLNKQFPHKIAVKVAYDNALAHKIEAGADMFLMPSRYEPCGLNQIYSLK
                     YGTVPIVRATGGLDDTIEPWDARAGKGTGFKFTEYNGESLLLTIKQALQAFRDQTSWQ
                     ALMRNGMSKDFSWTTSAREYGKIYERVKQMRGAAQASTEKVLV"
     gene            complement(2268..2777)
                     /locus_tag="VEJ00_04390"
     CDS             complement(2268..2777)
                     /locus_tag="VEJ00_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00429.1"
                     /translation="MAGRATRIAARIVFFVSGAVSIAVGVVFWSMHGTELPYQAEWPL
                     FAGVPFLTGVVAVLSALLPRRWTGKVFGVAPDDPSLLTVPGKGWLVLAAAAYLIALVF
                     HLAPESWHPGQQWTFSLCIVCGITASMGPQDHSLVSILLMLAPINAAIYGSIGLTLGY
                     AWFFLRRRP"
BASE COUNT          601 a          964 c          840 g          528 t
ORIGIN      
        1 ggaggcacga acgcctccgg cggcagcgtg aacagcttct cgacgtgggc gtacaattgc
       61 gtcgtcgccg agagcattcc gtagtcgcgc ccgcccggcg aggccgcgat ccggtccgcc
      121 acctcccgct gcaccatgat cacgatcgtg tcgaagtact tcgcaaactc aaacagccgc
      181 agcagaatgt cggacgtaat gtagtaggga agattgccca caaccctgac cggctcgggc
      241 ttgatgtcta tgccgggccg ccccaggccg ggcttcggcc cgaagagtga atcaaaatcg
      301 atggtcagaa tgtcggcctc aaggatctcc acgttccgcg ccatgccgaa cttcaagcgc
      361 agctgcgctg ccagcacccg gtccacttcc acggcgatca gccgtcgcgc ccgtctggct
      421 agcagcgaag tcagaatgcc ccggcctggt ccgatttcga gcaccgtact ctgtgaaatg
      481 tcgcccagcg cgccgacgat gcccgcggct gagtcgtcgc tggccagaaa atgctgtccc
      541 aacttgggtt ttgcctgcgg acgccgggca gacgctacct ttggcacgtt gaccctcacg
      601 cactcgggaa gtagacttgc ccttcggtcc ccgggttttt gcccgatgga ccccggcccc
      661 gagactcatt catcatagcg tgcgcggcga acgggcgcac tctggaggtt ccgcgttcat
      721 gcacgtcgca ttcgttgctt ccgaaggcgt cccattttcc aagaccggcg gcctggctga
      781 tgtggttggc gccctgcccc gtgccctcgc ggccctgggc caccaggtca gcgtttacct
      841 gccgcgctat cgccagacca agctcgccga tccgcccacc gtcgtccgca gcatcaccat
      901 tccgtttgac gacaagtacc gcttcgcttc aattgttggt ggcgtgaatc agggcggcgt
      961 gcgcttctac tttgtcgagt acccgaaata cttcgaccgc gacgcactct acggcacgcc
     1021 cgccggcgac tatcccgata acgccgaacg cttcgccttg ttctcccgcg ctgtgctcga
     1081 agcttcgaag attctcggag tcccgcagat tttccactgc cacgactggc agtcggcgct
     1141 ggtgccggtt ctgctgcgca ccgtctacgc cgaggacccg gcgttccgcg aagcaggtac
     1201 ggttttcacc atacacaaca tgggatacca gggcctgttc ccgcccgata cgcttcccct
     1261 gctcatgttg ccctgggatc tgttcaccat gtcgaagatg gaattcttcg gccaggtgaa
     1321 cttcctcaag ggagctctga cgtactccga cttcatcacc acagtcagcc gcaagtacag
     1381 ccaggagatt cagaccgccg aatacggatt cggcctggaa ggcgtgttgc gcgaccgcgc
     1441 cgccacggtc acgggaatac tcaacggcgt cgactatgac gagtggagcc cgcagaccga
     1501 caagttcacg gcggcaacgt actcgccgca ggatttatcg ggcaaagcta aagacaagca
     1561 ggatctgctg gccgccttcg gtgcgaccaa agccgatccc aagctgcccg tgatcggaat
     1621 cgtctcgcgc tttgccgcgc agaaaggttt cgatctcatc gcccagatcg ccgaccgcct
     1681 ggcccgcgaa gagatgacca tcgtcgccct cggcgctggc gacaaagcct acgaagaact
     1741 tttcctccgc cttaacaagc agttcccgca caaaatcgcg gtcaaggtcg catacgacaa
     1801 cgcgctggcg cataagatcg aagccggcgc cgacatgttc ctcatgcctt cgcgctacga
     1861 gccctgcggc ctgaaccaga tctacagcct gaagtacggc acggtgccga tcgtccgcgc
     1921 cactggcggc ctcgatgaca ccatcgaacc ctgggacgcc cgcgccggca agggaaccgg
     1981 cttcaagttc accgaataca acggcgaatc gctgctgctc accatcaagc aggcgctgca
     2041 ggccttccgc gaccaaacgt cgtggcaggc gctcatgcgc aacggcatga gcaaggactt
     2101 ttcctggacc acctccgccc gcgagtacgg gaaaatttac gaacgggtga agcagatgcg
     2161 gggcgcggcg caggcctcaa cagagaaagt actggtctga tttcaaattg agttgatggt
     2221 cagcgactcc cccgtggcat gaccacgtca tcgagttgga tcgcttctca aggccgtcgg
     2281 cgaagaaaga accaggcgta ccccagcgta agcccgatcg agccgtagat tgccgcgttg
     2341 atcggcgcca gcatcagcag gatcgaaacc agcgagtgat cctgagggcc catcgaggct
     2401 gtgattccgc agacaatgca cagagagaac gtccactgct gcccgggatg ccagctttct
     2461 ggagcgaggt ggaaaaccag cgcgatcagg taagcggcgg ccgccaggac cagccacccc
     2521 ttgcccggta ccgtcagcag tgagggatca tcgggcgcga ctccgaacac ttttccggtc
     2581 catcggcgcg gcagcaaagc actgagcacc gcaaccacgc cggtaaggaa tgggaccccg
     2641 gcgaataggg gccactccgc ctgatacggc agctcggtcc catgcatcga ccagaacacg
     2701 actcccactg cgatcgagac ggctccgctg acgaagaaca cgatccgggc agcgattctt
     2761 gtagcgcggc cggccatagc gcagcattct agcagcgggc ttcggttcca aggttatctc
     2821 gcacttcttc acatcagttc agatcgcttc agaccgactc catggacatt cttcggcagg
     2881 caaggcaaga tcgcatgcag ttgtggggaa cgagagtaag caagaagcca atc
//
LOCUS       DASPUL010000187         5939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_853646, whole genome shotgun sequence.
ACCESSION   DASPUL010000187 DASPUL010000000
VERSION     DASPUL010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5939)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5939
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853646"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VEJ00_04395"
     CDS             <1..696
                     /locus_tag="VEJ00_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXX00430.1"
                     /translation="FNLQVQRGETLVLLGRSGSGKTSTLKLINHLLVPTGGQVCVKGV
                     STTESDVIHLRRSIGYVIQDVGLFPHFTVERNIGLVPRIEGWAVERIRARVDELMQLV
                     GLAPELLSRYPHQLSGGQRQRVGVARALAADPEILLMDEPFGALDPLTRDDLQREFSS
                     LQKRLCKTVVFVTHDLREAVRLGSRIALMEAAKLVAVLPPDEFLRSNDPWAAAYVRAF
                     GEGLEAARNRGTS"
     gene            693..1325
                     /locus_tag="VEJ00_04400"
     CDS             693..1325
                     /locus_tag="VEJ00_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX00431.1"
                     /translation="MNVFQFIQQNQSQVLELTLEHLWLVGISTLLAALIGIPLGVAIA
                     QRDAFSKPVLAGANIIQTIPSLALFGFLLPLPWLGTSADRLAILALTLYALLPIIRNT
                     YTGIRGVDPAVVEAGRGMGLTDSQLLFQVQLPLALSVILSGVRTALVIAVGLATIAAA
                     IGAGGLGEFIFRGLAMVDNRVILAGAIPAAILALLADFGVGWLEKRLRPR"
     gene            1322..2233
                     /locus_tag="VEJ00_04405"
     CDS             1322..2233
                     /locus_tag="VEJ00_04405"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HXX00432.1"
                     /translation="MTAHWHQPRLSTLCLLVVGFSILTVSSCSREHTDRVVIGSKNFT
                     ESLILGEIIAQELEAHTNLKVERRFYLAGTYICHQAIRAGRIDVYPEYTGTALNAILN
                     QSGGNEEQIYERVKREYEQNFDLTLGQPFGFDDTFAMEIRGEDARRLNLKTLSQAAAF
                     APHWRAGFGYEFMERPDGYRGLAATYGLHFAEPPRIMDLGLLARALRDHQIDMAAGNT
                     TDGLIPALDLFVLEDDKHYFPPYQAVPVVREQSLQQHPEISQVLALLSDKISDADMRD
                     LNYSVDGQHRDVPQVVRDFLRRKGLRP"
     gene            2402..3730
                     /locus_tag="VEJ00_04410"
     CDS             2402..3730
                     /locus_tag="VEJ00_04410"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXX00433.1"
                     /translation="MTTAPNAVSVGHDLLERMSDARRKSDELFRVVKADSLYERPIPE
                     RHRIIFYVGHLEAFDWNLLHERLFGLKSFHPEFDRLFAFGIDPVGGGLPSDQPSDWPS
                     LQSVRDYVAGIRSALDQRLADGIPESITLSDVDSNTLLNVAIEHRLMHVETLAYMLHQ
                     LPLDRKVRQVDAPLAASAPVAHRKVSIPSGQATLGLPGDGATFGWDNEFEIHTADVPE
                     FEIDRYMVTNAQYLEFMQSGGYETRDFWSDDDWNWKESNGIAHPVFWKKAKGQWLYRT
                     MFDEIPLPLDWPVYVSHAEASAYARWAGKALPTEQEWHRAAYGSNEGRERLYPWGREA
                     ADPNFGNFDFNRWDPAPVNAFPAGRSAFGVEGLLGNGWEWSSTVFAPFQGFAPFPFYR
                     GYSADFFDGKHFVMKGGSARTGACMLRPTFRNWFQSHYQYVYTGFRCVER"
     gene            3745..4713
                     /gene="egtD"
                     /locus_tag="VEJ00_04415"
     CDS             3745..4713
                     /gene="egtD"
                     /locus_tag="VEJ00_04415"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HXX00434.1"
                     /translation="MLVHAIAANATYEFATEVRAGLAQPDQKELPSKYLYDEVGSALF
                     EVISHLPEYGLTRADERLLRRHADDIVDRVAGPVAVAELGSGSGKKTRWILEALCRRQ
                     RTSYYPVEISRSALLMCERELRDIDCISIVGFEREYLDGLLEVAACRRQGQRLLVLFL
                     GSTIGNFDRPAGIRFLDEVRRILEPGDSLLLGTDLEKPRAQLLRAYDDSLGVTAAFNL
                     NLLARINRELDADFDLGQFTHVARINDDARSVEMHLLSKRRQTVCIPAAEVVIEFLEG
                     ETIWTESSHKYSAAEIPGMARAAGFDCAAQWIDEPWPFAENLLVAV"
     gene            complement(4715..4936)
                     /locus_tag="VEJ00_04420"
     CDS             complement(4715..4936)
                     /locus_tag="VEJ00_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00435.1"
                     /translation="MLRLDYLSCVLTIVSTVLVGRRCWEGWALATVNSVLMCIIGVRT
                     AQLGFVPANVFCIVVSAINLRAWRKTGPS"
     gene            5052..5324
                     /locus_tag="VEJ00_04425"
     CDS             5052..5324
                     /locus_tag="VEJ00_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HXX00436.1"
                     /translation="MPPKCTHLDQIKVRTTNKHVCEECVKMGDTWVHLRLCLICGHVG
                     CCDSSKNKHATKHFHKSQHPLIRSIEPGETWMWCYVDEMEPGELVA"
     gene            5466..>5939
                     /locus_tag="VEJ00_04430"
     CDS             5466..>5939
                     /locus_tag="VEJ00_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXX00437.1"
                     /translation="MAKPILLSVDDDSDVLRAIERDLRSQYGADYRVIGSDSPEAALE
                     MLRELKLRNDGVALLLADQRMPRMDGVGFLKEARQIFPDAKRALLTAYADTNAAISAI
                     NQADVDYFFLKPWDPPAERLYPQLDDLLGDWRANYRPAFEGIRVLGTRWSPKCYEL"
BASE COUNT         1177 a         1781 c         1743 g         1238 t
ORIGIN      
        1 ttcaatctgc aagtgcaacg tggcgaaacg ctcgtcctgc tcggacgcag cggctcgggg
       61 aaaacctcaa ctctcaagct gatcaatcac cttctggtgc ccactggcgg gcaggtttgc
      121 gtcaaaggcg tttccacgac cgagagcgat gtcattcacc tgcgccgcag catcggctac
      181 gtgattcagg acgtaggtct gtttccgcat ttcacggtgg agcggaacat cggccttgta
      241 ccgcgaatcg agggatgggc tgtcgagcga attcgcgcgc gggttgacga gttaatgcaa
      301 ctggttggac tcgcgcccga gctcctgtcg cggtatccgc accagctttc cggcggacag
      361 cggcaacgcg tcggcgtggc gcgggccttg gcagcggatc cggagattct tctcatggac
      421 gagcctttcg gcgcactcga tccgctcacc cgcgacgacc tgcagcgcga attctcatct
      481 ctccagaagc gcttgtgcaa gacagtggtc ttcgttaccc acgacctgcg cgaggccgtg
      541 aggttgggat cgcgcattgc cctgatggaa gcagcgaagc tggtcgccgt tctcccgccg
      601 gatgaattct tgcggtcgaa cgatccctgg gccgctgctt atgtccgagc cttcggagaa
      661 gggctggaag ctgcccgcaa tcgaggcacg tcatgaatgt ctttcaattc attcagcaga
      721 accaaagcca ggtgttggag ctcacgctcg aacacctctg gctggtgggg atttccacgc
      781 tgctcgcggc gttgattgga atacccctcg gtgtcgccat cgctcagcgg gacgctttca
      841 gcaagcctgt gctggcgggc gccaacatca tccagacgat tcccagcctc gcactcttcg
      901 ggtttctgct cccgctcccg tggctaggaa cgagcgccga ccgcctcgcg atcctggcgc
      961 tcacccttta tgcactgttg ccgatcattc gcaataccta caccggtatc cgcggagtcg
     1021 acccggccgt cgtcgaagcc gggcgcggca tgggcctcac cgactctcag cttttattcc
     1081 aggtacaact gccgctggct ctgagtgtga tcctctccgg cgttcgcacg gcgctggtca
     1141 ttgcggtcgg tctggccaca atcgcagctg cgatcggcgc cggaggcctg ggcgaattca
     1201 tttttcgcgg gctggcgatg gtcgacaacc gggtcattct ggccggagcg atcccggccg
     1261 cgatcctggc actgctggca gattttggcg tcggttggct cgagaaacgc ctgcgaccgc
     1321 gatgacagcg cactggcatc aaccgcggct ttcgactctc tgcctgctcg tcgtgggttt
     1381 cagcatcctg acggtgtcct cttgttcacg ggagcacacg gatcgagtcg taattggctc
     1441 caagaacttc accgagtccc tgattctggg cgaaatcatt gctcaggagc tggaagcgca
     1501 cacgaacctg aaagtagaac ggcgcttcta tctggcagga acgtacatct gccatcaggc
     1561 aatacgggcg ggacgaatcg acgtctatcc cgaatacact ggcaccgcgc tcaacgcgat
     1621 tctcaaccaa agcggcggca atgaagagca gatctatgag cgtgtgaagc gagagtacga
     1681 gcaaaacttc gatctgaccc tcggccagcc tttcggcttc gacgatacct ttgcgatgga
     1741 gatccgcggg gaagatgcgc gacgcctgaa cctcaaaacg ctgtcgcagg ccgccgcgtt
     1801 tgccccacac tggcgtgccg gattcggcta cgagttcatg gagcgtcccg atggatatcg
     1861 cggcctggcg gccacctacg ggctgcattt tgcggaaccg ccgcgcatca tggacctcgg
     1921 cctgctggct cgggcgttgc gcgatcatca gattgacatg gcagcgggta acacgactga
     1981 cggcctgatt cccgcgctcg atctattcgt cctcgaagac gacaagcact atttcccacc
     2041 ctaccaggct gtgccggtgg tgcgcgaaca atctctgcaa cagcatcccg aaatctccca
     2101 ggtccttgcc ttactgtcgg acaagatttc tgacgcagac atgcgcgatc tgaactactc
     2161 cgtcgacggc cagcatcgcg atgtgccaca agtggtgcgg gatttcctgc gaaggaaggg
     2221 actccggccc tgattgcgta gaagctctcg cggcctcata aaccggctct gaaaaggcag
     2281 ggttttcaac cccgccctgt gactggtcat ccaacccgga tgcctgaaaa attgtttgtt
     2341 tcctggtgct actttggccc gggacggaca tctaaggtga acgtggctgg ggaggctagc
     2401 aatgaccacc gcgcccaacg ctgtgtccgt cggtcacgac ctgcttgagc ggatgtcgga
     2461 cgctcgccgc aaaagcgacg agctctttcg tgtcgtaaaa gctgattctc tctacgaacg
     2521 gcccattccc gagcgccatc gcatcatctt ctatgtgggg cacttggaag cattcgattg
     2581 gaacctgctc cacgaacgcc ttttcggcct gaagagcttc catcctgaat ttgaccggtt
     2641 attcgccttt ggcattgatc cagtgggcgg cggtttgccc tcggatcagc cttccgactg
     2701 gccttccttg caaagtgtgc gtgactatgt ggctggaatc cgctctgcgc tcgatcaacg
     2761 gctggccgat gggattccag agtcgataac actatccgac gttgactcga acactcttct
     2821 caacgtcgcc atcgaacacc gtctcatgca tgtcgaaacg ctggcctaca tgctgcatca
     2881 gttgccgctg gaccgtaagg tccggcaggt cgatgcgccg ttggccgcgt ctgcgcctgt
     2941 agcgcaccgc aaggtctcaa ttccatccgg gcaggcgacg ctcggtctgc cgggcgatgg
     3001 tgcgaccttc ggctgggaca acgaattcga aattcacaca gccgacgttc ccgagtttga
     3061 gatcgatcgc tacatggtga cgaatgcgca gtatctcgaa ttcatgcagt ctggcggcta
     3121 cgagactcgc gatttctgga gcgacgacga ttggaactgg aaagagtcga acggaatcgc
     3181 tcatcctgtg ttctggaaga aagcaaaagg acaatggctg taccgcacca tgtttgacga
     3241 gattccgttg cccttggatt ggcccgtcta cgtgagtcac gccgaggcct cggcttatgc
     3301 gcgctgggca gggaaggctc ttcccacaga acaggagtgg catcgcgctg catacgggag
     3361 caacgaaggc cgggaacggc tgtacccttg gggcagggaa gctgccgatc cgaattttgg
     3421 caatttcgat ttcaatcgct gggatcccgc accagtcaat gcttttcccg cgggccggag
     3481 cgctttcggt gttgaaggcc tgttgggcaa cgggtgggaa tggtcctcca cggtcttcgc
     3541 accattccaa ggtttcgcac cttttccttt ctaccgcggg tactcggccg atttcttcga
     3601 cggcaagcac tttgtcatga aaggcggctc ggcacgcacg ggggcgtgca tgctgcggcc
     3661 tacgttccgc aactggttcc agagtcacta ccaatacgtg tatacgggct tccgctgcgt
     3721 cgaacgctga tcaaggagtc acaaatgtta gtccatgcca tcgcagccaa tgccacctac
     3781 gagttcgcaa ccgaagttcg cgccgggctt gcacagcctg accagaagga actgccctcg
     3841 aagtacctgt acgacgaagt aggctcagcg cttttcgagg tgatcagcca tctccccgag
     3901 tatggcctga cgcgagccga cgagcggctg ttgcgccggc atgcggatga catcgtggac
     3961 cgcgtcgccg gaccggttgc ggtcgctgag ttgggaagcg gcagcggcaa gaaaactcgc
     4021 tggatcctcg aggcgctctg tcgccgccag cgcacttcgt actacccggt ggagatttcg
     4081 cgctcggctc tgctcatgtg cgagcgcgaa ctccgggata tcgactgcat cagcattgtg
     4141 ggttttgagc gcgaatatct ggatggcttg ctggaagtgg cggcctgccg gcggcagggc
     4201 cagcgcttgc tggtcctgtt tctaggcagc accatcggga actttgatcg gcctgcgggc
     4261 ataaggtttt tggatgaagt ccgccggatc ctcgagcctg gcgattctct gctgcttggc
     4321 accgacctcg aaaaacccag agcccaactg ctgcgcgcct atgacgacag cctgggcgtg
     4381 acagcagcct tcaatctcaa tctgctggct cgcatcaatc gtgaacttga cgccgacttt
     4441 gacctgggcc agttcaccca tgtggctagg atcaacgacg acgcgcgcag tgtggagatg
     4501 cacctcctgt cgaagcggcg gcagaccgtc tgcattccgg ccgctgaagt cgtgatcgag
     4561 ttcctcgaag gagagaccat ctggacggaa agcagccaca agtattcagc tgcagaaatt
     4621 cctggcatgg ctcgcgcagc cggattcgac tgcgcggctc agtggatcga cgagccgtgg
     4681 ccctttgccg agaacctgct tgtggcggtg tgactcagga aggccccgtc ttgcgccagg
     4741 cgcgcagatt gatggcgctg accacgatgc agaacacgtt ggccgggacg aaccccagtt
     4801 gtgcggttcg gactccaatg atacacatga gtacactatt cacggtcgcc agcgcccagc
     4861 cttcccagca ccggcgaccg acaagaactg tggacacgat ggtcaacacg caggacagat
     4921 aatcaagccg caacacaaat catggtcctc cgcccaccat tctccggaat aaaaccgcgc
     4981 gccgtcagtg attgtgccct ttcgtcacat gacgcgagtc acttgacgaa tccatttctc
     5041 aggaggaact aatgccgccc aaatgcacgc atctcgacca gatcaaggtc cgcaccacca
     5101 acaagcacgt ttgcgaagag tgcgtcaaaa tgggagacac ctgggtccac ctgcgcctgt
     5161 gtctcatctg tggtcatgtg ggctgctgcg attcgtcgaa gaacaaacat gccaccaagc
     5221 acttccacaa gtcgcagcac ccgctgatcc ggtccatcga accgggagag acgtggatgt
     5281 ggtgctatgt tgacgagatg gagccgggcg aactggtagc gtgaaaacag ggcatgaggg
     5341 ctgcggaaac gaaagccgcg accaacaggt aagtgcgacc agaagagatc ttctgaatct
     5401 aactgttttc gtgcgcttct ggcagtttct tcaaaccttc ttcgatctgg gggcaggcag
     5461 cgatcatggc aaaaccgatt ctcttgagcg tggatgacga ctccgatgtc ttgcgcgcga
     5521 ttgagcgcga tctgcgctcg cagtacggcg ccgactaccg cgtcatcggc agcgattcgc
     5581 ctgaagcggc gctggagatg ctacgcgagc tgaagctccg caatgacggg gtcgcgctcc
     5641 tgctggcgga tcagcgcatg ccccgcatgg atggcgtggg attcttaaaa gaggcgaggc
     5701 agattttccc cgacgccaag cgggccctcc ttactgccta cgccgacacc aatgcggcca
     5761 ttagcgccat caaccaggcc gacgtggact acttttttct caaaccttgg gaccctcccg
     5821 cggagcgctt gtatccccag ctcgacgacc tgctcggtga ctggcgggcc aactaccggc
     5881 cggcatttga gggaattcga gtgctcggca cacgatggtc tcccaaatgt tatgagctg
//
LOCUS       DASPUL010000188         4012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1263301, whole genome shotgun sequence.
ACCESSION   DASPUL010000188 DASPUL010000000
VERSION     DASPUL010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263301"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VEJ00_04435"
     CDS             <1..855
                     /locus_tag="VEJ00_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HXX00438.1"
                     /translation="RGDIVMATAPASDVMVPQPYLVREVSKETSDTFTLRLAPEGANG
                     SSFQPGQFSMLWVFGVGELPISISGDPNVHDQLVYTVRSVGQATNALVNQSVGAGVGV
                     RGPFGKGWPMEAARGKDVVVAAGGIGLAPLRGVIYEVLHHREEYGRLVILYGARSPRD
                     LLYRKELAAWARQRETQVLVTVDYGGMSWRGHVGVVTTLFKYARLKPANSVAMVCGPE
                     IMMRFVARELESHGLGRNDIYLSMERNMKCAVGFCGHCQYGPHFICKDGPVFTYNQMK
                     PLLEKYEL"
     gene            845..1654
                     /locus_tag="VEJ00_04440"
     CDS             845..1654
                     /locus_tag="VEJ00_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00439.1"
                     /translation="MSSETKSKSSSPSKRPRIGVFKFASCDGCQLSLLDAEDELLSVV
                     GAVEIAYFPEATRTMLKGPYDIGLVEGSITTHHDAERIQEVRRKCKTLITIGACATAG
                     GIQALRNWKDVDEFVRVVYASPQFISTLKLSTPIAEHVPVDFELRGCPINKYQLIELI
                     TATLAGRKPNVPTYSVCMECKRKENVCISVARGVPCLGPVTQAGCGALCPSYDRGCYG
                     CYGPMETPNTASLTSQFRILGQSDEQVSRAFRSFNAWAWPFRKASEECERA"
     gene            1651..2112
                     /locus_tag="VEJ00_04445"
     CDS             1651..2112
                     /locus_tag="VEJ00_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HXX00440.1"
                     /translation="MNGSGSRTIKVDTLARVEGEGALYIKMAGERVTDVKLRIYEPPR
                     MFEAFLRGRHYSEAPDITARICGICPIAYQMSAVHAIERALGIRIDPAVRLLRRLFYC
                     GEWIESHALHVYMLHAPDFLGYPDVIEMAKDHRAVVEKALRLKKIGNSIVH"
     gene            2167..2949
                     /locus_tag="VEJ00_04450"
     CDS             2167..2949
                     /locus_tag="VEJ00_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HXX00441.1"
                     /translation="MPGRRKLRELVSDLEWGLEASLEAVRWTSSLQFPDFEQDYEFVA
                     LRHPEEYPFNEGRLVSNRGLDIDAAEYEDRFVELHVKHSNALHSVIRGRGSYLVGPLA
                     RFNLNFDKLPGVAQKVALEAGLKPPVKNLFRSIVVRAVELVFAFAEALRVIREYEPPS
                     APRVIVPDRAGVGQAITEAPRGILYHRYAVDEKGLIVTAKIIPPTSQNQRRIEDDLRE
                     YAAQLAAWPLEEATWKCEQAVRCYDPCISCATHFLKVEIDRE"
     gene            complement(2961..3443)
                     /locus_tag="VEJ00_04455"
     CDS             complement(2961..3443)
                     /locus_tag="VEJ00_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00442.1"
                     /translation="MRLFSSANFSRDSLYDVFDSALDFVRDSTAYFKLLAFNPMHSQL
                     RGQTTERTRELDRLGNADPVCGCRRPGAPHGMLPIPYVHNARWGTSHHGIDDDNVIRA
                     RPGIYQFGRFAGADFCRNAESAQALDGDDSGTVVPALAIAAADNQHVVDPVGVIGRVA
                     "
     gene            complement(3468..3815)
                     /locus_tag="VEJ00_04460"
     CDS             complement(3468..3815)
                     /locus_tag="VEJ00_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXX00443.1"
                     /translation="MPSEPMQIERLDRGVLSFRGPLTMENVPPFLNAVRREGAPTMIL
                     DFSGVPYLDSSGLGSLVSAYTSCAKAGRRIALTGVNQRVQKVFEITKVEQVFLIFPTL
                     SDALEAFTNAGTA"
BASE COUNT          779 a         1171 c         1284 g          778 t
ORIGIN      
        1 cgaggtgaca ttgtgatggc gacggcgccc gctagcgacg tgatggtccc gcagccttac
       61 ctggtgcgcg aggtgtcgaa agagacctcc gataccttca ctttgcggct cgcccctgag
      121 ggagcgaatg gcagttcttt ccagccggga caattcagca tgctgtgggt gtttggcgtc
      181 ggcgaactgc ccatctcgat cagcggcgat cccaacgttc acgatcagtt ggtgtacacg
      241 gtgcgctcgg taggccaggc aaccaatgca ctggtcaacc agagcgtcgg ggcgggcgtg
      301 ggcgttcgcg gccccttcgg caagggctgg ccgatggagg ccgcccgcgg caaggacgta
      361 gtggtcgcag cgggcggcat cgggctggcg ccgctgcgcg gggtgatcta cgaggtgctg
      421 catcatcgcg aggaatatgg ccggctggtc attctgtatg gggcgcgcag tccgcgagac
      481 ctcttgtacc gcaaagaact cgccgcctgg gcgcggcaac gcgaaaccca ggtgctggtt
      541 acggtggact acggcggcat gagctggcgc ggacatgtgg gcgtggtcac gacccttttc
      601 aagtacgcgc ggttgaaacc ggcgaactcg gtggcgatgg tgtgcggtcc ggagatcatg
      661 atgaggtttg tcgcccgcga actggaatcg catgggctcg gccgcaatga catctatctt
      721 tcgatggaac gcaacatgaa atgcgccgtc ggcttttgcg ggcactgcca gtacggaccg
      781 catttcattt gcaaagacgg ccccgttttt acttataacc agatgaagcc gctgctggag
      841 aagtatgagc tctgaaacca aatcgaaatc cagctcaccc tcaaaacgtc cgcggattgg
      901 cgtgttcaag ttcgcctcct gcgacggctg ccagttgagc ctgcttgatg ccgaagacga
      961 actgctgagt gtggtcggcg cggtcgagat cgcctacttc ccggaggcga cgcggaccat
     1021 gctgaagggg ccgtacgaca tcggtttggt ggaaggatcg atcaccacgc accacgatgc
     1081 cgagcgtatt caggaggttc ggcggaagtg taaaacgcta atcacgattg gagcctgtgc
     1141 cacggcgggt ggcattcagg cgctgcgcaa ctggaaggac gttgacgagt ttgtccgggt
     1201 cgtgtatgcc tcgcctcagt tcatcagcac gctgaagtta tcgactccga tcgccgagca
     1261 cgtcccagtg gatttcgagt tgcgcggctg ccccatcaat aaatatcagt taattgagct
     1321 catcacggcg acgctggccg gccgcaagcc gaacgtccct acctacagcg tgtgcatgga
     1381 gtgcaagcgg aaagaaaacg tctgcatttc ggtggctcgc ggcgtgccgt gcctcggccc
     1441 ggtcacgcaa gcgggatgcg gcgctctttg cccgagctat gaccgcggtt gctacggctg
     1501 ctacgggccg atggaaacgc caaataccgc ttccctgacc agccagttcc gcatcctggg
     1561 ccagagtgac gagcaggttt cccgggcgtt ccgcagtttc aacgcctggg cgtggccgtt
     1621 ccgcaaggcc agcgaggagt gtgagcgcgc atgaacggga gcggcagcag aacgatcaaa
     1681 gtcgacacgc tggcccgggt ggagggtgaa ggcgcgctgt acatcaagat ggcgggcgag
     1741 cgcgtgaccg acgtgaaact gaggatctac gagccgccgc ggatgttcga agccttcctg
     1801 cgcggccgtc actattccga agcccccgac attacggcac gcatctgcgg catctgtccg
     1861 atcgcctacc agatgagcgc ggtgcatgcg atcgagcggg cgctgggtat caggatcgac
     1921 cccgccgtcc ggctgctgcg ccgccttttc tactgcggcg agtggatcga aagccacgcc
     1981 ctccatgtct acatgctgca cgcgccggat tttctcggct atcccgacgt catcgagatg
     2041 gccaaggacc atcgcgcggt ggtcgagaag gcgctccgct tgaagaagat cgggaactca
     2101 atcgtgcact agctgggcgg gcgcgagatt catcctatct cggccgcggt cggaggtttc
     2161 cacaaggtgc ccggcaggcg caaactccgc gaattggtca gcgatctgga gtggggactt
     2221 gaggcctcgc tggaagccgt gcgatggaca tcgagcctgc agtttcccga cttcgagcag
     2281 gactacgagt tcgtggcact gcgccatccc gaggaatatc cgttcaacga aggtcgcctg
     2341 gtctcgaatc gcggcctcga cattgacgcc gcggaatatg aggaccgttt tgtcgagctg
     2401 cacgtcaagc attcgaacgc gttgcattcg gtgatccgtg ggcgaggctc gtacctggtc
     2461 ggcccgctgg cgcgcttcaa cttgaatttt gacaagctgc cgggagtggc ccagaaagtt
     2521 gcgctcgaag ccggcctgaa accgccggtc aaaaacctgt tccgcagcat cgtggttcgc
     2581 gctgtcgaac tggtctttgc ctttgccgag gcgctgcgtg taatccgcga atacgaacca
     2641 ccctctgcgc cgcgcgttat cgtccctgac cgggccggag tgggccaggc gatcaccgag
     2701 gccccgcgcg gcattctcta tcaccgctac gcggtcgacg agaaagggct catcgtcacc
     2761 gccaaaatca ttccgccgac ttcgcagaac cagcgccgga tcgaggacga tctgcgcgag
     2821 tacgccgcgc agctggcggc gtggcccctc gaagaggcaa cctggaagtg cgagcaggca
     2881 gtgcgctgct atgacccgtg catttcctgc gccacgcatt ttttgaaagt cgaaatcgac
     2941 cgggagtagg aatccgcgag tcacgcaacc cggccgatca cgccgacggg atccacaaca
     3001 tgttgattat cggctgcggc aatcgccagc gcggggacga cggtgccgga atcatcgcca
     3061 tcgagcgcct gcgcgctctc ggcattcctg caaaaatctg caccggcgaa tcttccgaac
     3121 tgatagatgc ctggtctggc gcggatgacg ttatcgtcat cgatgccgtg gtgactggtg
     3181 ccccaacggg cattgtgcac gtatgggatg ggcagcatcc catgcggcgc tccaggtctt
     3241 cggcatccac acacgggatc ggcgttgccg aggcgatcga gctcgcgcgt acgctcggtc
     3301 gtctgccccc taagttgcga gtgcatggga ttgaaggcaa gaagtttgaa ataggcagtg
     3361 ctgtctcgca cgaagtcgag cgcgctatcg aacacgtcgt agagcgaatc gcgagagaag
     3421 ttggcgctgc taaataggcg catgcgtctc cagaaacatg gatcgcatta cgccgtcccg
     3481 gcgttggtga aggcctcgag cgcgtcggaa agcgtgggga aaatcaggaa gacctgttcg
     3541 accttggtaa tttcgaagac cttttgcacg cgctggttca cgcctgtaag tgcgatgcgg
     3601 cgtccggcct tggcgcagga cgtataggcg ctcaccagcg atcccaggcc tgacgaatcc
     3661 agatacggca cgcccgaaaa atccagaatc atggtcggag ctccttcgcg ccggaccgcg
     3721 ttcaggaacg gcggcacgtt ttccatggtc agcgggccac ggaagctcaa taccccccgg
     3781 tccaggcgct cgatctgcat cggctctgac ggcatagcaa gtgacggaga ttctagggca
     3841 gaaatgacgc gggttcaacc gggcaatgag cactgggcgt gtggttgcca gctggcctca
     3901 tttcgggagt cgccccctta ccgattcggg ctgtgagaac acacatttgt aattgttttg
     3961 tattttctgt ttacaggcag accgggcgta gctcggccca gttacgattg ct
//
LOCUS       DASPUL010000189         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1537277, whole genome shotgun sequence.
ACCESSION   DASPUL010000189 DASPUL010000000
VERSION     DASPUL010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537277"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1283)
                     /locus_tag="VEJ00_04465"
     CDS             complement(213..1283)
                     /locus_tag="VEJ00_04465"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HXX00444.1"
                     /translation="MRIGMLTGGGDCPGLNAVIRAVVRKGVFRYRDELVGFMEGWRGL
                     IEDQTMPLGLDAVSGILPRGGTILRTSRTNPSKRPDGIDRCMATLDKHKIDAMIAIGG
                     DDTLSVAQKLHEKGIKVVGVPKTIDNDLGGTDYTFGFDTACNVVCEAIDRVHTTAEAH
                     NRVMVVEVMGRDAGWIALYSGIAGGADVILIPERPFDVDKVAESIRQRHERGRYFSIV
                     VVAEGAKFSSESGNGAPILQDMGKDEFGHAKLGGVANILAREIEKRTGYETRAVVLGH
                     IQRGGSPSAFDRVLATRYGLGAIDMVHRGEFGCMAALRNNKIVSIPLKEAIARNRTVD
                     NEMIQIVDGLFEEIGVKEVASS"
     gene            complement(1470..1820)
                     /locus_tag="VEJ00_04470"
     CDS             complement(1470..1820)
                     /locus_tag="VEJ00_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00445.1"
                     /translation="MKTLTIATLITVLLGVGTALGQDKTGPAKAENGATHSAKVLMVS
                     GRVSADGKTLLTDIDSEWSVSNAETLKGLEGSLVTVRCYVDSEKTTIRVLSAKRADTG
                     STYASKHGDSAFRR"
     gene            1947..>2378
                     /locus_tag="VEJ00_04475"
     CDS             1947..>2378
                     /locus_tag="VEJ00_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HXX00446.1"
                     /translation="MGIALRHRIRHNAPADMNRTLSVFLWGAVALLGAGAVGVVAVHR
                     GEPINALWLVAAATCCYALGYRFYSKFVAAKILALDARRATPAERLENGRDFLVTNKW
                     VVFGHHFAAIAGPGPLVGPVLAAQFGYLPGTLWVLAGAVFAG"
BASE COUNT          428 a          764 c          700 g          486 t
ORIGIN      
        1 gtcgaagccg gagcatgccc ccgtcgcttc gatgagaaca agatcagcac tccggctgcg
       61 gccatcagca acagttcgat cgcagggagc agcacaggcg ccagccgggg atagagattc
      121 tgcatcgtgt gggcaagtcc agcactactt tatgcccgtg gcgaaaagat cagaagcggc
      181 ttaccggaga atacagggtt tcaatgggga gcctagctgc tggcgacttc cttgactccg
      241 atctcctcaa atagaccatc cacgatctgg atcatttcat tgtccacggt tcggttgcgg
      301 gcgatggcct ctttcagtgg gatggacaca atcttgttat tgcgcaaggc ggccatgcag
      361 ccgaattcgc cgcgatgcac catgtcgatg gcaccgagtc cgtaacgcgt ggccaaaacg
      421 cgatcgaagg cgctgggcga tccgccacgc tgaatatgtc ccagcacgac ggctcgggtt
      481 tcgtagccgg tccgcttctc gatttcccgc gccagaatat tcgccacgcc gcccagcttg
      541 gcgtgcccga attcgtcctt ccccatgtcc tgcagaattg gagctccgtt accgctctcg
      601 gaggaaaact tcgcgccttc cgccaccacg acgatcgaga agtaccgtcc gcgctcgtgc
      661 ctctgccgaa tggattccgc aaccttgtcg acatcgaacg gccgctccgg gatcagaatc
      721 acatccgcgc cgccggcaat gccgctgtag agcgcgatcc agcccgcatc ccgtcccatg
      781 acttccacca ccatcacgcg attgtgggct tcggccgtgg tgtggactcg gtcgatcgcc
      841 tcgcatacca cgttgcacgc cgtatcaaat ccgaacgtgt agtcggtgcc gcccaggtcg
      901 ttgtcgatgg tcttaggaac gccgacaact ttgatgcctt tctcgtggag cttttgtgcg
      961 accgacagcg tatcgtcgcc tccgattgcg atcatggcat cgatcttgtg cttgtcgagc
     1021 gtggccatgc agcgatcgat tccgtccggt cgcttggagg gattcgtccg ggacgtgcgc
     1081 aggatggttc cgccgcgggg caggattccg ctcaccgcgt ccaggcccag cggcatcgtc
     1141 tggtcttcga tcagcccccg ccagccctcc atgaacccca ccagttcgtc gcggtagcga
     1201 aagaccccct tgcgcacgac tgcgcggatg accgcattca gcccggggca atcgccgccg
     1261 ccggttaaca tgccgattct catggtcagc tccccatatc aggtatgagt tcggaacggt
     1321 gtccttggaa tgcgccccgg ggcagggcat gacgaaatgg cccgaaacac aaggggagca
     1381 atcgttcgga aaaactagct taacatcaaa tcgaggcagg ctggaacccg agcgcgcgct
     1441 tgggcgctcc ggcggcgggc atggcaaatc tagcgccgga aggcggaatc cccgtgtttg
     1501 gaggcgtagg tcgagccagt gtcggcccgc ttcgccgaga gtacccgaat cgtcgttttt
     1561 tcgctgtcca cgtaacagcg aaccgtgacc aggctgcctt ccagaccttt cagagtttcg
     1621 gcgttgctca cgctccactc cgagtcgatg tcggtcagca gcgtcttccc gtcggcgctc
     1681 actcgcccgg aaaccatcaa cactttggca gaatgagtcg ccccgttctc cgcttttgcc
     1741 gggccggtct tgtcctggcc caacgcggtc cccactccca gcagaacggt gatcagcgtc
     1801 gctatggtca aagtcttcat tggatccccc cggggtgaaa ccccaccact tgcacagatc
     1861 ttagggcgag taccggaggc acggaaattc aaaattctta tggccggcat tcaaaaagcc
     1921 gacagctgcg gcgcttcgtc actggaatgg gcattgcgct ccgccaccgg attcgccaca
     1981 atgctccggc ggatatgaat cggacgctgt cagtttttct ctggggagcc gtagctctgc
     2041 tcggcgcagg cgcggtgggc gtggtcgcgg tgcaccgcgg cgagccgatc aacgccctct
     2101 ggctggtggc cgccgcgaca tgttgttatg ccttggggta ccggttctac agcaagttcg
     2161 tcgcggccaa gattctggcc ctcgatgcac gccgcgcgac gcctgccgag cgcctggaaa
     2221 acggtcgcga ctttctcgtc accaacaaat gggtcgtctt cggccatcac tttgccgcca
     2281 tcgccggtcc gggcccgctc gtgggacctg tgctggccgc gcagttcggc tatcttcccg
     2341 gcacgttgtg ggtgctggcg ggcgccgtct tcgccggc
//
LOCUS       DASPUL010000190         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1949006, whole genome shotgun sequence.
ACCESSION   DASPUL010000190 DASPUL010000000
VERSION     DASPUL010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1949006"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..2161)
                     /gene="shc"
                     /locus_tag="VEJ00_04480"
     CDS             complement(182..2161)
                     /gene="shc"
                     /locus_tag="VEJ00_04480"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HXX00447.1"
                     /translation="MDMNSPDFNTASTSLGSSPQLRFGKIEELASRVGAAIAAARKRL
                     FAQQHEEGYWCGELEADTTLESDYILLHSLLGTGNPERFQKAANWILRHQNEDGGWSI
                     YASGPSNISASVKAYFGLKLAGYSADHPALQRARKKILELGGVTEVNTFTKIYLCFFG
                     QYDYDAVPAVPPEIVLFPNWFWFNIYEISSWSRAILVPLSICYAKKPFKKIPREMGVE
                     ELFVGGMHKSRMHLHWAKKLVSWRNFFLVIDRVTHWFERVHVRPLRSIALRSAEKWML
                     ARFEMSDGLGAIYPSMMNAIIALRCLGYSLDDPQVIRALDEFEKLGIEEDDTFRMQPC
                     MSPVWDTAYALFALGEAGTPANDRRMVKCADWILQKQVRNVGDWKVKNRKGQPGGWYF
                     EFNNEFYPDVDDSAMVCLALSRVEHPNGRYQRESVQRAIDWIFSMQCKNGGWASFDKD
                     NDRMIFQYIPFADHNAMLDPATVDITGRILEMLATYGYDINHRAVKRAIQFIRKEQEP
                     DGSWFGRWGVNYIYGTALVLRGLEAIGMDNHEPCIQQGAEWLRMVQNPDGGWGETVGS
                     YDDPTLRGQGDSTPSQTAWAILGLLAAGDTRSDSVARGVAYLLRTQSKDGSWDEPFFT
                     GTGFPRVFYLKYHLYREYFPLLALTTYQKVMEKQS"
     gene            complement(2267..>2474)
                     /locus_tag="VEJ00_04485"
     CDS             complement(2267..>2474)
                     /locus_tag="VEJ00_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00448.1"
                     /translation="WLKGVKTIALTAGASAPEHLVEETVTFLRGKGFGNVQELEVMPE
                     NVRFGLPPEIVEAIAAAPASASAD"
BASE COUNT          481 a          753 c          734 g          506 t
ORIGIN      
        1 ctggtggggc ggacgagcag gcgcagatct gcaccctgtt cggcaaggat gctcgcgacg
       61 tgggagccga ggaacccggt agcgccagtg acgaaggctt tcaagagaag tacccagttg
      121 tcagtcaaac acaacaaagc cgatctcgaa aaactggcaa ctgagttccg ggtacttgca
      181 gctaactctg cttttccatc accttctgat acgtggtcag cgccagcagc ggaaaatact
      241 cgcggtatag gtgatacttc aggtagaaca cgcgggggaa gccggtgccg gtgaaaaacg
      301 gctcatccca tgaaccgtct ttgctctgcg tgcgcaacaa ataggcgacg ccgcgcgcaa
      361 ccgaatcgct gcgcgtgtcg ccggcggcaa gcaggccgag gatggcccat gcggtctgcg
      421 acggcgtgct gtcgccctgg ccgcgcagcg ttgggtcgtc gtaggagccg acggtctcac
      481 cccagccgcc gtccgggttc tgcaccatgc gcaaccattc ggcgccctgc tgaatgcaag
      541 gctcgtggtt gtccatgccg atcgcttcca gaccgcgcag caccagcgcg gtgccataga
      601 tgtagttgac gccccagcgg ccgaaccagc ttccgtccgg ctcctgctcc ttgcgaatga
      661 actggatcgc ccgcttcact gctcggtggt ttatgtcgta gccataagtc gcaagcatct
      721 ccaggatgcg gcccgtgata tcgactgtcg ccggatcgag catggcgttg tgatcggcaa
      781 acgggatgta ctggaagatc attcggtcgt tgtctttgtc gaacgaggcc cagccgccgt
      841 tcttgcactg catggagaag atccagtcaa tcgcccgctg caccgattct cgctgatagc
      901 gcccgttggg atgttccacg cggctcaggg ccaggcagac catggcgctg tcgtcgacgt
      961 cagggtaaaa ctcgttgttg aactcgaagt accagccgcc gggctggcct ttccggttct
     1021 tcaccttcca gtcgcccacg ttgcgcacct gcttttgcag gatccaatcg gcacacttca
     1081 ccatcctgcg atcgttggct ggtgtgcctg cctcgcccag cgcgaatagc gcgtacgccg
     1141 tatcccacac cggcgacatg cagggctgca tgcggaaagt gtcgtcctct tcgatgccca
     1201 gcttttcaaa ttcatcgagc gcacgaatga cctgcggatc gtccagcgag tatccgaggc
     1261 aacgcagcgc gatgatggcg ttcatcatcg agggataaat ggcgcccagg ccgtcgctca
     1321 tctcgaagcg cgccagcatc cacttctcgg cgcttctcaa agcgatcgag cgcagcggcc
     1381 gcacgtgcac gcgctcaaac cagtgcgtaa cgcggtcgat gacaaggaag aaattccgcc
     1441 aggaaaccag cttcttcgcc cagtgcagat gcatgcgtga cttgtgcatg ccgccgacga
     1501 aaagttcttc gacgcccatc tcgcgcggaa tcttcttgaa cggtttcttg gcgtagcaga
     1561 tcgacagggg cacgaggatg gcgcgcgacc aggaagaaat ctcgtagatg ttgaaccaga
     1621 accagttggg aaacagcacg atctccggcg gaaccgccgg gacggcgtcg tagtcgtatt
     1681 gcccgaaaaa gcagagatag atcttggtga acgtgttgac ttcggtcacg ccaccgagtt
     1741 cgagaatctt cttgcgggca cgctgcaggg caggatggtc agcactgtat ccggcgagtt
     1801 tcagaccgaa ataggccttc accgaagcgc tgatgttcga cggccctgag gcatagatgc
     1861 tccagcctcc gtcctcgttc tgatggcgca gaatccagtt ggcggctttc tggaagcgct
     1921 ccggattgcc ggttccaagg agcgagtgca gcagaatgta gtcggactcg agcgttgtat
     1981 cggcttcgag ttcgccgcac cagtaacctt cctcgtgctg ctgggcaaac aagcgcttcc
     2041 gtgccgccgc gatcgcagcc cccacgcggc tggcgagctc ttcgattttt ccgaagcgca
     2101 gttgcggact ggagcctagc gatgtggaag ccgtgttgaa gtcgggggag ttcatatcca
     2161 tcagttcact gctccatctg tcggacgcgg ccctcagggg ctaaagccct gattctcttt
     2221 gtcgtctttg cggtacgagt aaactcgtac ccttcccgtt cgtccattaa tccgcgctcg
     2281 cgctggccgg cgccgccgcg atcgcttcca cgatctcggg aggcaggccg aagcgcacgt
     2341 tttccggcat cacttcgagt tcctgcacgt tgccaaatcc cttgccgcgc aaaaaggtca
     2401 cggtttcttc gaccaggtgt tcgggcgcgg aagcgcccgc ggtcagcgcg atcgtcttga
     2461 cgcctttgag ccac
//
LOCUS       DASPUL010000191         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_34932, whole genome shotgun sequence.
ACCESSION   DASPUL010000191 DASPUL010000000
VERSION     DASPUL010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34932"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VEJ00_04490"
     CDS             <1..603
                     /locus_tag="VEJ00_04490"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HXX00449.1"
                     /translation="EVKRNWEALKVEERKQREDAQESGRKHDAGIGQRSSILANISSA
                     MPSLLEAYKLSSRAAQTGFDWPDIEGLFDKLHEETNELREHLKEFPAPGPRPQGRGVA
                     GAGRTVIPEDLQVRLEEEVGDLLFVLVNIARYLSLDPESALRKTNRKFRRRFQWMEAR
                     LQESGRSPEQVPMEELESLWQQAKAAEQKRDMQPVQEKQA"
     gene            600..1373
                     /locus_tag="VEJ00_04495"
     CDS             600..1373
                     /locus_tag="VEJ00_04495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX00450.1"
                     /translation="MSSDSIVLRRCHGLDEMRACVALQKEVWNFSDAELVPLRMFVVA
                     DKVGGQVMGAFDGTTMVGFALSVPGTRTGHVYLHSHMLAVRKDYRNSGLGRRLKLMQR
                     EDALARGIELIEWTFDPLEIKNAYLNLEKLGAIARRYNINQYGITSSPLQGGLPSDRL
                     IAEWWLKSKRVETLLATGKNPPFRRELEIVVPAKIYEWKATAETRGNAQQVQEHNREQ
                     FLRAFAQGLAVLGYDRDAAGNGKFLLGRWDEPCSYASQP"
     gene            complement(1458..>2472)
                     /locus_tag="VEJ00_04500"
     CDS             complement(1458..>2472)
                     /locus_tag="VEJ00_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXX00451.1"
                     /translation="VVKANGGTTAWTDKHPSYEWTNGPSGKGVDDFYGPEINSTPIAL
                     PQFPGCSPVNFADNTPDDGWTTDLRNVQCYDLLHVQAVLNQIAGLSHDGSKKLGVPSL
                     FGTNFQSVSVGEKLAGNGYTDVLGTPSAGLASELDFVDQQLGRMVQALEKNGLLDSTL
                     IIIGAKHGQSPIDVKKRVGIGGGQPAATIGPADAFDISDDGSLIWLTDQELTASVVAN
                     LSQPANQKKLGIQEIFAGNSLKNKWDDPGSDPRTPDIVLKVNTGVIFTGGSKIAEHGG
                     MNEDDVHVALLVSFAGLQPQSVKTAVLNQQVAPTIIKALGFDPTQLQAVQQEGIQPLP
                     FLF"
BASE COUNT          492 a          752 c          764 g          464 t
ORIGIN      
        1 gaagtcaagc gcaactggga agctctcaag gtcgaagaac gtaagcagcg agaggatgcc
       61 caagaaagcg gccgcaagca tgatgccggg attggacaga gaagttcgat ccttgcgaat
      121 atctcctccg ccatgccatc gctgctcgaa gcctacaagc tcagttcgag ggccgcgcag
      181 acaggcttcg actggcccga tatcgaaggc ttatttgaca agctccacga ggagaccaac
      241 gagctgcgcg agcacctgaa ggaatttccc gcgcccggtc cgcgcccgca aggcaggggc
      301 gtagccgggg ccgggcgaac ggtgatcccc gaagaccttc aagttcgact tgaagaagaa
      361 gtcggcgacc tgttgtttgt actcgtcaac atcgcccgct acctctcgct cgatccggaa
      421 tcggcattgc gcaaaacgaa ccgcaaattc cgccgccgct ttcagtggat ggaggcgcgg
      481 ctgcaggaat ccggccgctc gcccgagcag gttcccatgg aggagctgga atccctctgg
      541 cagcaggcga aggcggccga gcagaagcgc gacatgcagc ctgttcagga gaagcaggcg
      601 tgagctccga cagcattgtg ctccgccgat gtcacggcct cgacgaaatg cgggcgtgcg
      661 tcgctttgca aaaagaagtc tggaacttca gcgacgccga actggtgccg ttgcgcatgt
      721 tcgtggtggc cgacaaggtt ggaggccagg tcatgggagc ttttgacggc accaccatgg
      781 tcggcttcgc gctctcggtg cccggcaccc gcacgggcca cgtttacctg cactcgcaca
      841 tgctggcggt gcgcaaagat tatcgcaaca gcggtctcgg ccggcggctc aagctgatgc
      901 agcgcgaaga cgccctggcc cgcggcatcg agctgatcga atggaccttt gatccgctgg
      961 agatcaagaa cgcctacctc aatctcgaaa agctgggcgc gatcgcgcgc cgctacaaca
     1021 tcaaccagta cgggattacc tcatcccccc tgcaaggcgg attgccgagc gatcgtctga
     1081 tcgccgaatg gtggctgaaa tcgaaacgcg tcgaaacgct gctggcgacg gggaagaacc
     1141 cgccattccg acgcgaactc gagatcgtgg ttcccgccaa gatctacgag tggaaagcca
     1201 cggccgaaac tcgtggcaac gcgcaacagg tgcaagagca caatcgggag cagttcctgc
     1261 gcgcgttcgc gcaaggtctc gcggtcttgg gctatgatcg cgatgccgcg ggaaacggca
     1321 agttcctgct cggccgatgg gacgaaccct gctcctacgc atcgcagccc tgatcgctag
     1381 caacgtcctc cctccggaga aagagaaaag cggcggaccc ttcggatccg ccgcctgggt
     1441 tttcaacgtc caactgatca gaacaggaac ggtagcggct gaatcccctc ctgctgtaca
     1501 gcctgcaact gggtgggatc gaagcccagc gccttgatga tggtgggcgc gacctgctgg
     1561 ttcaggactg ccgtcttgac cgactgcggc tgcagtccgg cgaacgacac cagcagagcc
     1621 acgtgcacgt cgtcttcatt cataccgccg tgctcggcga ttttcgatcc gccggtgaaa
     1681 atgactcccg tgttgacctt gagcacgatg tcgggcgtgc ggggatcgct acccggatca
     1741 tcccatttgt tcttcagcga gtttccggcg aagatctcct ggatgccgag ctttttctga
     1801 ttggcgggct ggctaaggtt cgccaccacg ctggcggtga gctcctgatc cgtcagccag
     1861 atcagcgagc cgtcatcgga gatgtcaaat gcgtcggctg ggccaatcgt cgctgccggc
     1921 tgtccgccgc cgattccaac gcgcttcttt acgtcgatcg gagattggcc atgcttggcg
     1981 ccgatgatga tcagcgttga gtccagcagc ccgttcttct cgagcgcctg caccatgcgt
     2041 cccagttgct gatccacgaa gtccagttcc gaggccaacc cggcggaagg tgtgccgaga
     2101 acatcggtgt agccattgcc cgccagcttc tcgccaacgc tgaccgattg gaagttggtc
     2161 ccaaacagac tcggaacccc gagcttcttg ctgccgtcgt ggctcagtcc ggcaatctgg
     2221 ttgagcacgg cctgcacgtg cagcaggtcg tagcactgga cgttgcgcag gtcggtggtc
     2281 cagccgtcgt cgggcgtgtt gtccgcgaag ttcacgggag agcagccagg aaattgcggc
     2341 agcgcgatcg gagtggaatt gatctcggga ccatagaagt cgtccacgcc tttgccgctg
     2401 ggtccgttcg tccattcata ggaggggtgc ttgtcggtcc aggcggtcgt gccgccattg
     2461 gccttcacca ct
//
LOCUS       DASPUL010000192         4184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_308842, whole genome shotgun sequence.
ACCESSION   DASPUL010000192 DASPUL010000000
VERSION     DASPUL010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308842"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VEJ00_04505"
     CDS             complement(<1..482)
                     /locus_tag="VEJ00_04505"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="starvation-sensing protein; maybe involved in
                     homoserine lactone degradation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional D-altronate/D-mannonate
                     dehydratase"
                     /protein_id="HXX00452.1"
                     /translation="MKITDAKMLICSPGRNFVTLKVVTDQGIYGLGDATLNGRELAVA
                     SYLQDHLIPSLIGRDPFQTEDIWQYFYRGAYWRRGPVTMTAVGAIDTALWDIKGKALN
                     TPVYNLLGGKSRDRVLVYGHANGKDIEETTEEVGKYIAMGYRAVRAQTGIPGLPSTYG
                     V"
     gene            complement(543..2006)
                     /locus_tag="VEJ00_04510"
     CDS             complement(543..2006)
                     /locus_tag="VEJ00_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HXX00453.1"
                     /translation="MRQFGLTIALAASACLSGCAVGPRYQRPLVQSPPTWKTQGPWRE
                     AAPKDAFPKGSWWEVFHDEELNGYEQQLLKANQSLAAARDRLDQARSLARVASAGLFP
                     VASTDTNASRNRYSGNRPQIVTSGPALTQSLFEIPFALNYEVDLFGRARKTLQAANAS
                     LQATAADLGNVQLVLTAELAADYFSLRELDAEYAVVQESVKIQEEGLALVNHRHEGGI
                     ASGLDVAQQQTLLDSTVTQLSLLQQQRAQFEHAMAVLTGNPASAFSVAVAPLHAAPPP
                     VPLGMPSDLLERRPDIATAERTMAQQNALVGVAKTSFYPQLTISGGGGVESTALSSLI
                     SAPSAFWSLGGDLLQPIFNGGKNRATLAAQKSAYDESVANYRESVLTAFQQVEDGLSD
                     LNSLSQAAASQNAAVADARRALDIANNRYVGGLTNFLDVITAQSVLLTNERLSTQLLG
                     QQMVASVFLVKALGGGWDASAIQNEQVHPKPVQTVQP"
     gene            complement(2003..3232)
                     /locus_tag="VEJ00_04515"
     CDS             complement(2003..3232)
                     /locus_tag="VEJ00_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXX00454.1"
                     /translation="MPAETEIDSPREESYRSSPQITPPPELPAAPARKALFIVAIVVV
                     ILAVGGAASMLSRIRAGRALAKETDADSIATVVVVHPVAEKPDEELVLPASLQAYEES
                     PIYARTNGYLLKWYKDIGSRVTQGELLADIDTPEVDQELSQARATRQQSAAQLDIAKI
                     SADRWQNLRKTDSVSQQETDTQTNAYQQAVANLAAADANVRRLEQLESFKHVYAPFSG
                     ILIKRNVDPGALINAGSSGTELFIIAKVDPLRVFTNVPQAYSPAITTGMQAYITLPEI
                     AGEKFRGTVARTADAIDPATRTLLTEVDVPNKDGRLLRGSFGEVHFAPKINTAKVTVP
                     VNAMLFRQEGAQLAVVGPDNKVQLRPITIGRDYGTNLEILGGVSVEDRIIINPSDSLE
                     DGQKVNVAGENQEGQRP"
     gene            complement(3283..>4184)
                     /locus_tag="VEJ00_04520"
     CDS             complement(3283..>4184)
                     /locus_tag="VEJ00_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXX00455.1"
                     /translation="SGSFQTAPSFWLNPRNGVTYQMVVQSPQYRMTSLQDLMDMPVTD
                     PRVTNTQVLGNLAQLSPVTRPAVVSHYQIEPVMDVYASTQGRDLGAVAKDTNKVLEPF
                     MNHLPRGTRIVVRGQVETMRSSFFGLGIGLLGAILLVYFLIVVNFQSWLDPFVIITAL
                     PGALAGIAWILLLTHTTLSVPSLTGAVMCMGVATANSILMVSFARDRMQVGVPALQAA
                     LEAGYTRIRPVLMTALAMVIGMLPMSLGLGEGGEQNAPLGRAVIGGLMFATAATLFFV
                     PCVFTMVHGRRAAGDANREIVMD"
BASE COUNT          814 a         1291 c         1265 g          814 t
ORIGIN      
        1 acgccatagg tcgacggcag accgggaata cccgtctggg cgcgaacggc gcgataaccc
       61 atcgcgatgt acttgccaac ttcctcggtg gtctcctcga tgtccttccc gttcgcatgg
      121 ccgtagacga gaacgcggtc acggctcttg cctcccagaa ggttgtaaac aggagtattc
      181 agcgcttttc ctttgatatc ccagagcgcc gtgtcaatgg cgccgacggc cgtcatcgta
      241 accggccctc gccgccagta cgcgccgcgg tagaagtatt gccagatgtc ctcggtctga
      301 aacggatcgc ggccgatcag ggacggaatg agatgatcct gcaggtagga agccacggcc
      361 agttcgcgcc cgttcaacgt ggcatcgccc agtccgtaga tcccctggtc ggttacaacc
      421 ttcagcgtta cgaagttccg cccgggagaa cagatcagca ttttcgcatc tgtaatcttc
      481 aaaactcctc cagtaccggg ctggaaacgg gcttgccaaa tccttcttgc accctgaccg
      541 cgctacggct gcaccgtctg aacgggcttg ggatggacct gctcgttctg aatcgcggat
      601 gcatcccagc ctccccccag agctttcacc aggaagaccg aagcgaccat ctgctggccc
      661 agaagctggg ttgacagccg ttcgttggtc aacaatacag actgtgctgt gatgacgtcg
      721 agaaagttcg tcagaccgcc gacgtagcgg ttgttggcga tgtcgagcgc gcgacgcgcg
      781 tctgcgaccg ccgcattctg gctggccgcg gcctgcgaca aggagttgag gtccgacaaa
      841 ccgtcttcga cttgttgaaa tgccgtgagt accgattcgc ggtagttcgc tacggattcg
      901 tcataggcgg atttctgagc agcgagagtg gcgcgattct ttcctccgtt gaaaatcggt
      961 tgcaacagat cgcctcccag cgaccagaac gcgctgggag cgctgatcag cgaactgaga
     1021 gccgtgcttt caaccccgcc gccgcccgaa atcgtgagtt gggggtagaa cgaagtcttg
     1081 gcgacgccca cgagagcgtt ctgctgggcc atcgttcgtt ctgcggtggc aatatcggga
     1141 cgtcgttcga gaaggtcgga aggcatcccc agaggaaccg gcgggggagc ggcgtgcagc
     1201 ggcgcgaccg caacactgaa ggccgaagcc ggatttccag tgagcacggc catggcgtgc
     1261 tcaaactggg cacgctgctg ctgcagcagg gagagctgcg tcacggtcga gtccagcagc
     1321 gtctgctgtt gcgccacgtc caggcctgag gcaattcctc cctcatggcg atgattcacc
     1381 agagccagac cttcctcctg gatctttacc gattcctgca cgaccgcata ttcggcatcg
     1441 agctcacgca ggctgaagta gtcggcggcg agctccgccg taaggaccag ctgtacgttg
     1501 ccaaggtcgg ccgcagtggc ctgcaggctg gcgttggcgg cttgcagggt tttccgcgcc
     1561 cggccgaaca agtcgacttc atagttcaac gcgaacggaa tctcgaacaa gctttgtgta
     1621 agcgcggggc cggaagtcac gatctggggg cgattgccgg agtaccggtt gcgcgaggca
     1681 ttggtatcgg tgctggcaac gggaaagagc ccggcggaag cgactcgcgc cagcgagcgt
     1741 gcctgatcca ggcggtccct ggctgccgca agggactgat tcgcctttag cagttgctgc
     1801 tcgtaaccgt tgagctcctc gtcgtgaaaa acctcccacc agctgccctt gggaaaggca
     1861 tccttggggg cggcctcacg ccatgggcct tgcgtcttcc acgtgggggg actttgaacc
     1921 agcgggcgct gatagcgtgg cccgacggcg catcctgaca aacacgcgct tgcagccagc
     1981 gcaatggtca gcccgaactg cctcacggac gctgcccttc ctggttctcg ccagctacat
     2041 tcaccttctg gccatcttcg agcgagtcgg aggggttgat gatgatgcgg tcctcgacgc
     2101 tcacgccgcc caggatttcg aggtttgtgc cgtagtcgcg cccaatcgtg atggggcgga
     2161 gttgcacctt gttatcgggc ccaactaccg ccaactgcgc accttcctgc cgaaacagca
     2221 tcgcattcac gggcacggtg actttggccg tgttgatctt gggcgcgaag tgcacttcgc
     2281 cgaacgatcc gcggagcagg cggccgtcct tgttggggac gtcgacttcg gtcagcaagg
     2341 ttcgtgtcgc tggatcgatc gcgtcggcgg tgcgcgccac ggttccccgg aacttctccc
     2401 cggcaatctc tggcagtgtg atataggcct gcatccccgt cgtaatggcg ggcgagtagg
     2461 cttgcggaac gttggtgaac acgcgcagcg gatcgacctt ggcaatgatg aagagctctg
     2521 tgcccgacga cccggcgttg atcagcgcac ctggatctac attgcgcttg atcaggatgc
     2581 cggagaacgg agcatagacg tgtttgaacg attccagttg ctccagccgc cggacgttgg
     2641 cgtcggctgc ggccaggttg gccactgcct gctggtaagc attcgtctgg gtatcggttt
     2701 cttgctgaga aaccgagtcc gtcttgcgca ggttctgcca gcggtcagcg gaaatcttcg
     2761 caatgtccag ctgcgcggcc gactgctgcc gggtagcgcg cgcctgcgag agttcctgat
     2821 cgacctcggg agtatcgatg tccgccagca actcgccctg ggttacgcgg ctgccgatgt
     2881 ccttgtacca cttcagcaag tacccgttcg ttctggcata gatgggagac tcctcgtagg
     2941 cctgcaacga cgcaggcaga accaactctt catcgggctt ttcggcgaca ggatggacaa
     3001 cgacgaccgt ggcgatggag tcggcatccg tttccttggc cagggcgcgc ccggcgcgaa
     3061 tgcggctcaa catgctcgca gcgccgccca cagccaatat caccacaacg atcgccacga
     3121 tgaacaaagc cttgcgcgcc ggggccgcgg ggagttccgg cggcggcgtg atctgcggcg
     3181 aactccggta cgactcctcc ctgggtgaat cgatttctgt ctctgcaggc atgatccctc
     3241 gttatgaatg actacaaatg caactcccga aaatgttgcg aactagtcca tcacaatctc
     3301 cctgttcgcg tcccccgctg cgcgacggcc atgaaccata gtgaacacgc aagggacgaa
     3361 aaacagggtt gccgcggtcg caaacatcaa tccgccgatc accgcccgcc cgagcggggc
     3421 attctgctcg ccgccttcgc ccaaccccag agacatgggg agcatgccga tgaccatggc
     3481 cagagccgtc atcagcacgg ggcggatgcg ggtgtagccg gcctcgagcg cggcctgcaa
     3541 cgccggcacc cccacctgca tgcgatcgcg cgcgaacgaa accatcagga tggaattggc
     3601 agttgccact cccatgcaca tcacggcgcc cgtcagtgac ggaacgctca aggtcgtgtg
     3661 cgtcagcagc agaatccagg caattcccgc cagcgctccc ggaagagcgg tgatgatgac
     3721 gaacggatcc agccacgact gaaagttcac cacgatcagg aaatacacga gcaggatcgc
     3781 tcccagcagt ccgataccca ggccgaaaaa cgaagaccgc atcgtctcca cttgtcctcg
     3841 cacaacgatt cgcgtacccc gcggtagatg gttcatgaac ggttccagca ctttgttcgt
     3901 gtccttggct accgctccca gatcgcggcc ctgagtacta gcgtaaacgt ccatcaccgg
     3961 ctcgatctgg tagtgcgaaa ccactgccgg ccgggtaact ggcgacagct gcgccaggtt
     4021 gcccagcact tgtgtgttcg taacccgggg atccgtcacc ggcatgtcca tcaggtcctg
     4081 cagagacgtc atgcggtact ggggcgattg cacaaccatt tgataagtaa cgccattcct
     4141 tggattcagc cagaaactgg gagccgtctg aaagctgccg ctca
//
LOCUS       DASPUL010000193         3453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_990296, whole genome shotgun sequence.
ACCESSION   DASPUL010000193 DASPUL010000000
VERSION     DASPUL010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990296"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1596
                     /locus_tag="VEJ00_04525"
     CDS             <1..1596
                     /locus_tag="VEJ00_04525"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HXX00456.1"
                     /translation="EGLVGLITYMRTDSTRVAPEAIVEVREYVGKEYGPGYLPETPNT
                     YKEKKEAQAAHEAIRPTSAMRHPDQIKQYLKEDEFKVYKLIWQRFVASQINPAVFDQT
                     TVDIDAKNGNDSFWFRVTGSVLKFDGFLKVYKESTEGEDQEDEELKHKLPALEAGQKL
                     TLRELKPEQHFTEPPPRYNEASLVKELEERGIGRPSTYAAILSTIQERQYVQKLGGKF
                     VPTEIGLVVTDLLVENFRDIFDVQYTARLEEELDEIEEGKEKWQDALGEFYKKFQKDL
                     KYAEKHMENIKRMEKPTDEKCERCGAPLVIKWGKHGSFYACSTYDKEDPNTCTFTKEN
                     PINLPDLDSADMQETGAAEEYCENCGRVMVLKRGRFGQFMACTGYPDCKTTRRLDQGK
                     RVPDIPLDELCPKCGRNMMIRHGRYGEFTACSGYPDCKYVKQNFIGVKCPECKDGDLV
                     EKRARKGNTFYGCSSYPKCKFTSAHKPIAEKCPSCGSEYLVEKNLKAGPVIACPNKEC
                     DYERPAPPPPPDAAAGAAAPAHP"
     gene            1672..2133
                     /locus_tag="VEJ00_04530"
     CDS             1672..2133
                     /locus_tag="VEJ00_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00457.1"
                     /translation="MAKKPTAADAQLIIQLYDLRREAEMRKARHWWLVDFNPQSADDF
                     MKVAWAFGSQENAWLRQVSGYWGIVTSFVLQGVLNEDLFLQPGFSGELFFVYAKIHPF
                     VKEIRQKLDDPEFFLNIEKTVTRTKWGKNRLQFLMKRIALLREKTAERKAS"
     gene            2191..3090
                     /locus_tag="VEJ00_04535"
     CDS             2191..3090
                     /locus_tag="VEJ00_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXX00458.1"
                     /translation="MPEKTLPLRGHVALVTGGGRGIGRAIAQTFAAAGAAVAVLARSA
                     AELTETTGLIEGNGGRCLAFRADVTDSSGVRGAIQAIEKSLGPVDVLVNNAGTPKPFG
                     PLWETDPGEWWRGMEVNLLGPLLCTRFVLPGMISRRRGRIISVASGAGAMAMTHFSSY
                     VCSKTALVRFTECLALETKPHGIVAFAIAPGTVRTAMSEYSLNSPEGQKWLPWFRRIF
                     DEQFDVPAERPAHLVLELASGRADSLSGRFLSIHDDLDELIKNAAEIERRSLYLLKQE
                     RLPGGPTNSALASIMVAARDAAR"
BASE COUNT          776 a          998 c         1046 g          633 t
ORIGIN      
        1 gaaggcctgg tcggtctgat cacctacatg cgtaccgatt cgacccgggt agcgcccgag
       61 gcgatcgtcg aggttcgcga gtatgtgggc aaggaatatg ggcctgggta tttgcccgag
      121 acgccgaaca catataagga gaagaaagaa gcgcaggccg cccacgaagc gatacggccg
      181 acgtcggcca tgcgccatcc cgaccagatc aagcagtatt tgaaagaaga cgaattcaag
      241 gtctacaagc tgatctggca gcgcttcgtg gcctcgcaaa tcaatcctgc ggtgttcgat
      301 cagaccacgg tcgacattga tgccaagaat ggcaacgact ctttctggtt ccgcgtcacc
      361 ggttcggttc tcaaattcga cggcttcctc aaggtctaca aagagtcgac ggaaggcgaa
      421 gaccaggaag acgaagaact aaagcataag ctgcccgccc tcgaagccgg acaaaaactt
      481 acgctgcgcg aactgaaacc cgagcagcac ttcaccgagc cgccgccgcg ctacaacgag
      541 gcttcgctgg tgaaggaact cgaggaacgc ggcatcggcc ggccttctac ctacgctgcc
      601 attctttcaa cgattcagga gcggcagtac gtgcagaagc tgggcggaaa gtttgtgccg
      661 actgaaatcg gcctggttgt gaccgacctg ctggtcgaga acttccgcga cattttcgac
      721 gtgcaataca cggcgcgcct ggaagaggaa ctcgacgaaa tcgaagaggg caaggaaaaa
      781 tggcaggacg ccctcggcga gttctacaag aagttccaga aagatctcaa atatgccgaa
      841 aagcatatgg agaacatcaa gcggatggag aagcccacgg acgagaagtg cgagcgctgt
      901 ggcgcgccgc tggtgatcaa gtggggcaag cacggatcgt tctacgcctg cagcacctac
      961 gacaaagaag accccaatac ctgtaccttc acgaaagaaa atcccatcaa cctgcccgac
     1021 ctcgactcgg cggatatgca ggaaaccggc gcggccgagg aatactgcga aaactgcggc
     1081 cgcgtgatgg tgctcaaacg cggacgcttc ggtcagttca tggcgtgcac ggggtatccc
     1141 gattgcaaga ccacgcggcg gctggatcag ggtaagaggg ttcccgacat tccgctcgac
     1201 gaactttgcc cgaaatgcgg gcgcaacatg atgatccgcc acggccgcta tggcgaattc
     1261 accgcctgca gcggttatcc cgactgcaaa tatgtgaaac agaacttcat cggcgtgaag
     1321 tgccccgagt gcaaggacgg tgacctggtc gaaaagcgcg cacgcaaagg caacacgttc
     1381 tacggatgtt caagctatcc gaaatgcaag ttcacgtcag cgcacaagcc catcgccgag
     1441 aagtgcccaa gttgcggcag cgaatacctg gtcgagaaga atttgaaagc cggcccggtc
     1501 atcgcttgcc cgaacaagga atgcgactac gagcggccgg cgccaccacc tccgccggac
     1561 gcagcagccg gtgctgcggc gccggcccat ccctgaggat tttttgcctc ccagctgcgt
     1621 cttgggtcgt ctaagcttcg cgagtggtgt ccaactccaa cggaggaacc gatggccaag
     1681 aaaccgaccg cagccgatgc tcaactgatc attcaactct acgacctgcg ccgcgaagcc
     1741 gagatgcgca aggctcgtca ttggtggctg gtagacttca acccgcaaag cgccgacgac
     1801 ttcatgaaag tggcatgggc ttttgggtca caagagaacg cctggctacg gcaggtcagc
     1861 ggctattggg gcatcgtcac ttctttcgtg ctgcaaggcg tgctcaatga ggatctcttt
     1921 ctgcagcccg gattcagcgg cgagcttttc ttcgtctacg ccaagatcca tcccttcgtg
     1981 aaagagattc gccaaaaact cgacgaccct gagttcttcc tgaacattga aaaaactgtc
     2041 actcgcacga agtggggcaa aaaccggctg caattcctga tgaagcggat cgcccttttg
     2101 cgcgagaaga cggccgagcg caaggccagc tgagcccgtt aattttcgcg atttgttctc
     2161 ccatgctatg ctgccggcat gagtcaacag ttgcccgaaa aaaccttgcc cctgcgcggc
     2221 catgtcgctc tcgtcaccgg cggaggcaga ggcattggtc gcgcgattgc ccagaccttc
     2281 gcagccgccg gggctgcggt ggcggtattg gctcgttctg cagcggagct cacggagacg
     2341 accggcctga tcgagggcaa cggcggccga tgccttgcct ttcgtgccga tgtgaccgac
     2401 agcagcgggg ttcgcggcgc catccaggcg attgagaagt ctctgggccc ggtggatgtg
     2461 ctggtcaaca atgcaggcac gccaaagccc tttggccccc tctgggaaac ggaccccggc
     2521 gagtggtggc ggggcatgga agtgaacctg cttgggcccc tgctgtgtac gcgattcgtg
     2581 ctgcccggca tgatttctcg gcgcaggggc agaatcatca gcgtggcaag cggggccggg
     2641 gccatggcga tgacgcactt cagcagctac gtgtgcagca agaccgcgct ggtccggttt
     2701 accgaatgcc tggcgctgga aaccaaacca cacggcatcg tagctttcgc gattgctccg
     2761 ggcacagtca gaactgccat gagcgagtat tcgctgaact ctccggaggg gcagaaatgg
     2821 ctgccgtggt tccggcgaat tttcgacgaa cagttcgatg tgcccgcgga gcgcccggcg
     2881 caccttgttc tggagttggc ttccggccgg gcggacagcc tgtcgggccg atttttatcg
     2941 attcacgacg acctggacga gctcatcaag aacgcagccg aaatcgagcg acgcagcctc
     3001 tatttgttga aacaggaaag gcttccgggt ggaccaacga actccgcgct cgcgtccatc
     3061 atggtggcgg cgcgcgacgc tgcccggtag gcggccccta gcggcgacgt tgcgacgccc
     3121 cgacggaatg cagcctgctg aacctggatc atctgttatc ctaaaccgct gtgcggatgg
     3181 tctggtggtt cctgatcctg ggtgtaagta cgctggttgt ggtgtgcgtg gccatcgctc
     3241 tcgtcatgca tttgcgacga aacctccgca agggccatac ttcgcgcgac gacgccgggc
     3301 gtgaaaaaga tgagcgttga gggggacgat caggccctga cagggattcg acctccgcag
     3361 ataggggaaa cagccgtgtc aagaccgacg acccaggttc taagcactcc ctacggacgc
     3421 acgatggaag ctacgcgcca ggtggctttg gta
//
LOCUS       DASPUL010000194         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_169921, whole genome shotgun sequence.
ACCESSION   DASPUL010000194 DASPUL010000000
VERSION     DASPUL010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169921"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VEJ00_04540"
     CDS             complement(<1..1317)
                     /locus_tag="VEJ00_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00459.1"
                     /translation="MKPLASRGQSLPGLFSSVYALIFIAVAGLMPSCSKPAAPPQPAS
                     HPSDIKVEVRDGGPVVITTSTAEFQVLPSGFVQAALLKEGKRLTLDEPGVGSTGGSDS
                     IIHEGKELDFVPDFTQAKVLEASGKLGRGKRVEIPGRPLAAAGVPIERMLTVEAYDDF
                     PGIALVSAAYKNVGNDDFQIDQVWMQQHRFSSHDASAQPYDMWSFQGSSYDWGKDDVL
                     KLTRTSSQPNLMGEAVKGGYGGGIPVVAFWTRSMGEAIGHVETLPWTLSMPVKVEKAG
                     RVNASVTIPVNSALKPGETYSSPRSFVAVYTADFYEPLRMWSSVLQKEGWEIPKPSNE
                     AYNVSWCGWGYEFNVTPAQMLGTVPKLKEMGIKWATLDDRWFDTYGDWNPRKDTFPGD
                     SIKQMVDDFHKQGILAQLWWLPLGVEDGEGRWESHKYIVAQVVKDHP"
     gene            complement(1409..2413)
                     /locus_tag="VEJ00_04545"
     CDS             complement(1409..2413)
                     /locus_tag="VEJ00_04545"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HXX00460.1"
                     /translation="MRTTDLRVIFLLFFACLASFARSESYKYFRLGNKEDVQTKPTAG
                     VAMMGGGSDLDEAFRWLCKRGNGGDFLVLRARGDDDYNSYVNGLCKANSVATLIIPGR
                     EAAQDPAVAEIIRKAEVLFIAGGDQANYIRGWKGTPVQQAINDDVAEGKPIGGTSAGL
                     AVLGEFVYGALGDKPDDKDLASTDVLPNPYFDRVTLERDFLKIPLLDNLLTDSHFAKR
                     DRMGRTLGFLARIMKDGWSLSPREIAIDEKSAVLVEADGNATVVGTGKGAYFLRPAKA
                     PEVCEQSVPLTLRDVSVYRVSAGGHFDLPAWTGQGGTAYSLSVERGKIESTQEGSGVY
                     "
BASE COUNT          590 a          951 c          792 g          606 t
ORIGIN      
        1 gggatgatcc ttcaccacct gcgccacgat gtatttatgc gactcccacc gtccctcgcc
       61 atcctcgact cccagcggca accaccaaag ctgcgccagt atcccctgct tgtggaagtc
      121 atcgaccatc tgcttgatcg agtcgccggg gaatgtgtcc ttgcgcggat tccagtcgcc
      181 ataggtgtcg aaccagcgat catcgagcgt tgcccacttg atgcccatct ctttcagctt
      241 cggcaccgtg cccagcatct gcgccggagt cacgttgaac tcatatcccc acccgcacca
      301 gctgacgtta taagcctcgt tagacggctt gggaatttcc catccttctt tctgcagcac
      361 gctcgaccac atgcgcagcg gctcgtagaa gtcggccgtg tagacggcca caaaacttcg
      421 cggcgacgag taggtctcgc ctggcttgag cgccgaattg accgggattg tcacgctggc
      481 attcactcgt ccggcttttt ccaccttgac cggcatcgac agcgtccagg gcaaggtctc
      541 gacatgcccg atcgcctctc ccatcgaccg ggtccagaac gcgaccaccg gaatcccgcc
      601 accgtagcct cccttcacgg cttcgcccat caggttcggt tgcgacgagg tccgcgtcag
      661 cttgagcacg tcatccttgc cccagtcgta gcttgatccc tgaaatgacc acatgtcgta
      721 gggctgcgcc gacgcatcgt gggaactgaa ccggtgctgc tgcatccaca cctgatcgat
      781 ctggaagtca tcgttgccca cgttcttgta agccgcgctg accagcgcga taccgggaaa
      841 gtcgtcgtac gcttccaccg tgagcatccg ctcgatgggc acccctgccg cggccaacgg
      901 acgcccggga atctccacgc gcttccctcg gccgagtttg ccgctggcct cgaggacttt
      961 cgcctgcgtg aaatcgggca caaaatccag ttctttccct tcatgaatga tcgagtcgct
     1021 gccgcccgtc gatcccacgc ccggttcgtc gagcgtcagc cgcttgcctt ccttcagcag
     1081 tgccgcctgc acaaagccgg aaggaagaac ctgaaactca gccgtgctcg tcgtgatcac
     1141 gaccggcccg ccgtcacgca cttctacctt gatgtcggaa gggtgcgatg ccggctgggg
     1201 cggcgccgca ggtttggaac aactgggcat gagcccggcc accgcgatga agatcaatgc
     1261 atagacggac gagaacaacc caggaaggct ctgaccgcgg gatgccaagg gcttcatatt
     1321 cccccctctg aaacaccgta ttgtaacgaa agaaaaagaa atctttcgta actatacgcc
     1381 ggtcggcgcg atttccacca cttgatcgtc agtaaactcc gctgccctct tgtgtgcttt
     1441 caatcttgcc gcgctccacg gaaagcgaat acgctgtccc accctgtccg gtccacgcag
     1501 gcaaatcaaa atgccccccg gcgctcacgc ggtaaacgct gacgtcccgc aacgtgagcg
     1561 gcacactttg ttcgcagact tccggcgctt tcgccggccg cagaaaatac gctcctttcc
     1621 ctgtacccac cactgttgca ttgccgtcag cctccaccag caccgccgac ttctcgtcga
     1681 tggcaatctc ccgcggcgac aaactccagc catccttcat gatccgcgcc agaaagccta
     1741 gcgttcgccc catgcggtcg cgtttggcga agtgcgaatc cgtcagcagg ttgtccagca
     1801 gtggaatctt caggaaatcc cgctccagcg tgacgcggtc aaaatacgga ttcggcagca
     1861 catccgtcga cgccagatcc ttgtcatccg gcttgtcgcc cagagctccg tatacaaatt
     1921 cgcccagcac ggccaaaccc gcactggtcc cgccgatcgg cttgccctcc gccacatcgt
     1981 cgttgatcgc ctgctgcacg ggtgttccct tccagccgcg aatgtagttc gcctgatcac
     2041 cgcccgcaat gaataacacc tccgccttgc gaatgatctc ggccacggcc gggtcctgcg
     2101 ccgcttcgcg gccggggatg atcagcgtcg cgaccgagtt cgccttgcac aacccgttca
     2161 cgtaggagtt gtagtcatcg tctccccgcg ctcgcaggac gagaaaatcg cccccgttgc
     2221 cccgcttgca gagccagcgg aaagcttcat ccagatcgct gccgccgccc atcatcgcga
     2281 cgccggcggt gggcttcgtc tgaacgtctt ccttgtttcc gagccggaag tatttgtagg
     2341 actcggaccg agcgaaactt gccaggcaag cgaagaaaag gagaaatatc acccgaagat
     2401 cggtcgttct catgcaatct ctcctggggt tcaacagatt ccgggcttac ccaagtcgca
     2461 gggaccgcaa tgcgcggacg atggctgtag gcagccctga cagtaaagta aagaacgatc
     2521 tacaatagat tactcttggg gccggaaagc gtagtccgct cgccgtccat ggagaagtac
     2581 tctagctccc ctgtcgatct ttcccgttct cactcgtcta acaggtgatg accgataggg
     2641 gaaatccatc cggacccgac gtcggagcgc gcgggagacc ggagggctgc taagcgaggg
     2701 gggagaccaa gcgggggaga gattggagat ccggccggga actctgaatc ctttgctggt
     2761 tgggtaaaat cgatattgaa acgactgttt ttggccgcag aaccctccaa aatcaggtct
     2821 agacatcgcg tttgtgtttt gtttataccg gagaatgtat ggcgaagaga atgatcctga
     2881 tgctggcggt gatggccgcg gtgttgggtg ggctcggatt tttgaaatac cggcaggta
//
LOCUS       DASPUL010000195         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_169925, whole genome shotgun sequence.
ACCESSION   DASPUL010000195 DASPUL010000000
VERSION     DASPUL010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169925"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VEJ00_04550"
     CDS             complement(<1..413)
                     /locus_tag="VEJ00_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00461.1"
                     /translation="MKPLASRGQSLPGLFSSVCTLIFIAVAGLMPSCSKPAAPPQPAS
                     HPSDIKVEVRDGGPVVITTSTAEFQILPSGFLQAALLKDGKRLTLDEPGVGSTGGSDS
                     IIHEGKELDFVPDFTQAKVLEASGKLGRGKRVEIPG"
     gene            complement(505..1509)
                     /locus_tag="VEJ00_04555"
     CDS             complement(505..1509)
                     /locus_tag="VEJ00_04555"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HXX00462.1"
                     /translation="MRTTDLGVIFLLSFACLASSARAESYKYFRLGSKEDVQTKPTAG
                     VAMMGGGSDLDEAFRWLCKRGSGGDFLVLRARGDDDYNSYVNGLCKANSVATLIIPGR
                     EAAQDPAVAEIIRKAEVLFIAGGDQANYIRGWKGTPVQKAINDDVAEGKPIGGTSAGL
                     AVLGEFVYGALGDKPDDKDLASTDVLPNPYFDRVTLERDFLKIPQLDNLLTDSHFAKR
                     DRMGRTLGFLARIMKDGWSLSPREVAIDEKSALLVEADGKATVVGTGKGAYFLRPTQA
                     PEVCQQNVPLTLRDVSVYRVSAGGHFDLPAWTGQGGTAYSLSVERGKIESTQKGSGVY
                     "
BASE COUNT          417 a          640 c          559 g          414 t
ORIGIN      
        1 ccgggaatct ccacgcgctt ccctcggccg agtttgccgc tggcctcgag gactttcgcc
       61 tgcgtgaaat cgggcacaaa atccagttct ttcccttcat gaatgatcga gtcgctgccg
      121 ccggtagatc cgacgccggg ctcgtccagc gtcagccgct tgccgtcttt caacagagcc
      181 gcctggagaa acccggaagg aagaatctga aactcagccg tgctcgtcgt gatcacgacc
      241 ggtccgccgt ctcgcacttc gaccttgatg tcggaagggt gcgatgccgg ctggggcggc
      301 gccgccggtt tggaacaact gggcatgagc ccggccaccg cgatgaagat caatgtacag
      361 acggacgaga acaacccagg aaggctctga ccgcgggatg ccaagggctt catattcccc
      421 cctctaaaac accgtattgt aacgaaagaa aaagaaatct ttcgtaacta tacgccggtc
      481 ggcgcgattt ccaccacttg atcgtcagta aactccgctg cccttttgtg tgctttcaat
      541 cttgccgcgc tccacggaaa gcgaatacgc tgtcccaccc tgcccggtcc acgcaggcaa
      601 atcaaaatgc cccccggcgc tcacgcggta aacgctgacg tcccgcaacg tgagcggcac
      661 attctgctgg cagacctccg gcgcctgtgt cggccgcaga aagtacgctc cttttcctgt
      721 acccaccact gttgctttgc cgtctgcttc cacaagcagc gccgacttct catcgatagc
      781 cacttcccgc ggcgataaac tccagccatc cttcatgatc cgcgccagaa agcccagcgt
      841 tcgccccatg cggtcgcgtt tggcgaagtg cgaatccgtc agcaggttgt ccagctgggg
      901 aatcttcagg aaatcccgct ccagcgtgac gcggtcaaaa tacggattcg gcagcacatc
      961 cgtcgacgcc agatccttgt catccggctt gtcgcccagc gctccgtata caaattcgcc
     1021 cagcacggcc aaacccgcgc tggtcccgcc gatcggcttg ccctccgcca catcgtcgtt
     1081 gatcgccttc tgcacgggtg ttcccttcca gccgcgaatg tagttcgcct gatcaccgcc
     1141 cgcgatgaac aacacctccg ccttgcgaat gatctcggcc acggccgggt cctgcgccgc
     1201 ttcccggccg gggatgatca gcgtcgcgac cgagttcgcc ttgcacaacc cgttcacgta
     1261 ggagttgtag tcatcgtctc cgcgcgctcg caggacgaga aaatcgcccc cgctgccccg
     1321 cttgcagagc cagcggaaag cttcatccag atcgctgccg ccgcccatca tcgcgacgcc
     1381 ggcggtgggc ttcgtctgaa cgtcctcctt gctccccagc cggaagtatt tgtaggactc
     1441 ggcccgagcg ctacttgcca ggcaagcgaa ggaaaggaga aatatcaccc caagatcggt
     1501 cgttctcata caatctctcc tggcggtcca acagattccg ggcttaccca agtcgcaggg
     1561 accgcgcgga cgatggctgt aggcagccct gacagtaaag taaagaacga tctacaatag
     1621 actactcttg gggccggaag gcgtagtccg ctcgccgtcc atcgagaagt actctagctc
     1681 ccctgtatct ttcccgttct cactcgtcta acaggtgatg gccgataggg gaaatccatc
     1741 cggacccgac gtctgagcgc gcgggagacc ggagggctgc taagcgaggg gggagaccaa
     1801 gcgggggaga gattgaagat ccacgccggg aactgtgaat cctttgctac ttgggtaaaa
     1861 tcgatattga agagactgtt tttggccgta gaaccctcca aaatcaggtc tagacatcgc
     1921 gtttgtgttt tgtttatacc ggagaatgta tggcgaagag aatgatcctg atgctggcgg
     1981 tgatggccgc ggtgttgggt gggctcggat ttttgaaata ccggcaggta
//
LOCUS       DASPUL010000196         4691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1950324, whole genome shotgun sequence.
ACCESSION   DASPUL010000196 DASPUL010000000
VERSION     DASPUL010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950324"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VEJ00_04560"
     CDS             complement(<1..734)
                     /locus_tag="VEJ00_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HXX00463.1"
                     /translation="MESKTAEFQSLDRSARERGFRVVAIGGGTGLSTLLKGLKRFVLW
                     PAEEFSAAHPEAAVIRDLCAVVTVSDDGGSSGRLRKELNMLPPGDIRNCIVALSEDEA
                     LLSKLFRHRFDKGSGLEGHSFGNLFLAALTSLTSDFSEAVRLSSEILLTRGHIYPATT
                     SNIELEALMEDGTRVRGETKITASKGRIRELSLVPPDVEPMPQTLEAIANADLITIGP
                     GSLFTSLIPNLLVRGIADAIVASPAT"
     gene            complement(756..1331)
                     /locus_tag="VEJ00_04565"
     CDS             complement(756..1331)
                     /locus_tag="VEJ00_04565"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX00464.1"
                     /translation="MAQKPTAKKTVAEDPRYTQALQSYEAGLRAMQEHKFDKAKPHFQ
                     KVIAGPSKELADRANVHLNTCNQNLERAAATQFKTPEEHFDYAVSLMNIGDYVSAREH
                     LDKLLKQNPKGDYVIYGLAALDCLTGHVEDSLRRLDEALRLNPQLRFQARNDSDFQNL
                     AEDPRFTELLYPDPGAELGSANSDFPESEPA"
     gene            complement(1523..2002)
                     /locus_tag="VEJ00_04570"
     CDS             complement(1523..2002)
                     /locus_tag="VEJ00_04570"
                     /inference="COORDINATES: protein motif:HMM:NF041379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYCXC family (seleno)protein"
                     /protein_id="HXX00465.1"
                     /translation="MTGCSTIKMLYRSLALVVLFFVTLTMTAAWAAPPAEETGVPAYN
                     AGPPPKGTKLPPILDKTALWGADANPYQTHAYELAAKIPTVLHQQPCYCYCDRMGHNS
                     LHSCFESTHGAECATCLRELYYSYQQHKRGKTAAQIRAGIIRGEWKQIDLLQASTMN"
     gene            2167..2733
                     /gene="lepB"
                     /locus_tag="VEJ00_04575"
     CDS             2167..2733
                     /gene="lepB"
                     /locus_tag="VEJ00_04575"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXX00466.1"
                     /translation="MANASSENGRPGTENPPRAAAGFGVWVRDLIISVAIAAFVIIFI
                     YQPVKVEGVSMMPSLDDQERIFVNKFVYRLEPIERGDVVVFRYPPDPAKSYIKRVIGV
                     AGDRIRIDSGQVYVNDVPLQEDYVPPAYADTRSYPESVVPDGYYFVLGDHRSMSSDSR
                     EFGPVNQSYIYGKAAFGYWPMDKVGRVR"
     gene            2913..4043
                     /gene="carA"
                     /locus_tag="VEJ00_04580"
     CDS             2913..4043
                     /gene="carA"
                     /locus_tag="VEJ00_04580"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HXX00467.1"
                     /translation="MQAILALEDGRVFRGKGYGAKGECYGEVVFNTSITGYQEIFTDP
                     SYSGQIVVLTNPEIGNYGTNQEDNEATRPWIEGLIVREFSKVSSNWRSQQVAEEYLEQ
                     FKVPVLSDIDTRALVRHLRDHGVMRGVISTIESDPEKLVAKALSLPKMDGTDLAKEVS
                     TNRSYVWEVGERSHEPVAVVGVKDEPARFHVVAYDFGIKQNILRKLRGEGCKVTVVPA
                     QTLAEDVLALKPDGVFLSNGPGDPEPCTYAVDSIRRLMGRQPIFGICLGHQLTGLALG
                     GRTFKLKFGHHGGNHPVKQLKTGKIEITAHNHNFAVDPDSLAESEVELTHMDLNDQTL
                     EGLRHRNLPLFSVQYHPEAAPGPHDSHYLFKEFTKMMEEWKG"
     gene            4040..>4691
                     /locus_tag="VEJ00_04585"
     CDS             4040..>4691
                     /locus_tag="VEJ00_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00468.1"
                     /translation="MKHAGMAAVCWLLLIGFSNAQALTLYPGSDKTTKCPRNVAINVI
                     ADGKPLKDVKVEFCQTPQNQNCVSGTTGDDGKSKFLQLRPGNHSLHVTTKNGIIATEL
                     RFHTSHSSKASQFTVDMTGEIRFATQLIAYGDGLPIESRLQRFQGFTQDHAGSAVPEA
                     YIRVVRKADKQGENPILLYSDQDGHFSAHLEDGFYIAFFRRVGFVTQTVAFEISPQG"
BASE COUNT          971 a         1336 c         1424 g          960 t
ORIGIN      
        1 ttggtggcgg gcgaggcgac gatcgcatcg gcaatgcccc gcaccagcag atttggaatc
       61 aggctggtga agagcgaacc cgggccgatg gtgatcaggt cggcgttggc gatggcctcg
      121 agcgtctggg gcatgggctc cacgtcgggt ggaaccagcg acagctcgcg gatgcggccc
      181 ttgctcgccg tgatcttggt ttccccccgc acgcgcgtgc cgtcttccat cagcgcttcc
      241 agctcaatat tcgaagtcgt ggccgggtag atgtggccac gcgtgagaag aatctccgac
      301 gacaatcgca ccgcctcgga gaaatcagac gtgagcgagg tcagcgcggc cagaaacagg
      361 ttgccgaagc tgtggccttc cagtcccgag cctttgtcga agcggtgccg gaagagcttc
      421 gacaggagcg cctcgtcttc gctgagggcg acaatgcaat tgcgaatgtc gcccggcggg
      481 agcatgttga gctctttgcg aaggcgcccg ctggagccgc cgtcgtcgct gacggtcacc
      541 accgcgcata gatcgcgaat aacggccgcc tcgggatggg cagccgagaa ttcctccgcc
      601 ggccacagta caaagcgctt aagacccttc agcagggtgg aaagcccggt gccgccgccg
      661 atagcaacga cgcggaagcc gcgctcacga gcgctcctgt ccagggattg aaactcggcg
      721 gttttactct ccatcagccc gtcctcggag gcggtttacg cgggctcact ttcgggaaaa
      781 tcgctgttcg ccgagccaag ttctgcgccc ggatcaggat agagcagttc ggtgaagcgc
      841 gggtcctcgg ccaggttctg gaaatcggaa tcgtttcgtg cctggaaacg aagctgggga
      901 ttcagacgga gcgcttcgtc gagacgccgc agcgaatcct ccacgtggcc ggtgagacaa
      961 tcgagcgcag ccaggccgta gatgacgtag tcgcctttag gattctgctt caacagcttg
     1021 tccagatgct cgcgcgcgct gacgtagtcg ccgatgttca tcagcgacac ggcgtagtcg
     1081 aagtgctctt ctggcgtctt gaactgagtc gccgcggcac gctccaggtt ttggttgcag
     1141 gtgttgaggt gaacgttagc gcggtcggcc agttccttgc tcggtccggc aatcactttc
     1201 tggaagtgcg gcttggcctt gtcgaatttg tgttcttgca tggcgcgcag ccccgcttcg
     1261 tagctttgca gcgcctgcgt gtagcgcgga tcttccgcca cagtcttctt cgctgtaggt
     1321 ttctgagcca tgtgtaaaac tcttcctgtg ctcttggtaa tgggggactc cttatgtacc
     1381 aactatgaca acctgcttat gacagcctgc gacgccgagc aataaagtat cacctaaaga
     1441 gcgggtgcag ggcaatggcg gcagacgcct cccgggcaag cgagttcgct tctggtacct
     1501 gcgatttaga actggcggcg agctagttca tggttgaggc ctgcagcagg tcgatctgct
     1561 tccactcgcc gcggatgatc ccggcacgga tctgggctgc agtcttgcct cgcttatgtt
     1621 gctggtagga atagtaaagc tctctcaggc aggtggcgca ttccgcgcca tgggtgcttt
     1681 cgaagcagct gtgcaggctg ttgtgtccca tgcggtcgca atagcagtag caaggttgct
     1741 ggtggagcac agtgggaatc ttggccgcca gttcgtaggc gtgggtctga tagggattcg
     1801 cgtcggcgcc ccaaagtgcg gttttgtcga ggatcggggg cagcttcgtc ccttttggcg
     1861 gaggtccggc attataagcc ggaacgccgg tttcctcggc tggcggcgcc gcccaggcgg
     1921 ccgtcatggt caacgtgacg aaaaacagga ccaccagggc caagctgcgg tagagcatct
     1981 taattgtgga acaaccagtc atgaagtctc ctcgtgttcg ctcattgtaa cccgcaatca
     2041 ggacctatcc cgggatcgag cattgctccg ttcggtacaa cccgaagcgg aggttgcgcg
     2101 ttctgtatac aatggaaacg catgccagcg tcgggatcag agcaaaattc agccagccgg
     2161 gcagcaatgg cgaacgcctc cagcgagaat ggccgcccgg gcacggagaa tccgccgcgc
     2221 gcggcggcgg gcttcggcgt ctgggtgcgc gacctgatca tctcggtggc catcgccgcc
     2281 tttgtcatca tcttcatcta ccagccggta aaggtcgaag gcgtcagcat gatgccttcg
     2341 ttggacgatc aggaacgcat cttcgtcaac aagttcgtat atcggctgga accgattgag
     2401 cgcggggatg ttgtcgtgtt tcgctatccg cccgatccgg ccaagagcta catcaagcgc
     2461 gtgatcggag tggccggcga ccgcatccgc atcgacagcg gccaggtcta tgtgaacgac
     2521 gtgccgctgc aggaagacta cgtaccccct gcctatgccg atacccgctc ctaccctgaa
     2581 agtgttgtgc ccgacggata ctatttcgtt ctcggcgacc accggtccat gtcgagcgac
     2641 agccgtgagt tcggccccgt gaaccagagc tacatttacg gcaaggctgc gttcggatat
     2701 tggcccatgg ataaagtggg acgggttcgc tagttcgatc tcagcagcca agtcccagcc
     2761 tggttcgcag ttccagaaga accgtaagag ctgatttgag cggggctctg cgtggctcat
     2821 atccacagat ttctttgccg gcggtgcgtt ccggtctttc ccctgatctc tttccctgct
     2881 agacttctga aaatccactc tccggagcga gaatgcaggc tatccttgcg ctggaagacg
     2941 gccgcgtctt ccgaggcaaa ggctacggcg ccaaaggcga atgctacggc gaagtcgttt
     3001 ttaatacttc catcaccggc taccaggaaa ttttcaccga cccgtcgtac tcgggccaga
     3061 tcgtcgttct gaccaaccct gaaatcggca actacggcac gaaccaggaa gacaacgagg
     3121 ccacgcgccc ctggatcgaa ggcctgattg tgcgcgagtt ttcgaaggtc agctcgaact
     3181 ggcgctcgca gcaggtggct gaagaatatc tggagcagtt caaggtgccg gtgctgtcgg
     3241 atatcgacac acgcgcgctg gtgcggcatc tgcgcgatca cggcgtgatg cgcggcgtca
     3301 tctcgacgat tgaaagcgat cccgaaaaac ttgtggccaa ggcgctgtcg ctgcccaaga
     3361 tggacggcac cgaccttgct aaagaagtca gcaccaatcg ctcgtatgtg tgggaagtgg
     3421 gtgagcgctc gcacgagccg gttgcagtcg tcggagtgaa agacgagccg gcccgctttc
     3481 acgtggtcgc ctacgacttc ggcatcaagc agaacattct gcgcaaactg cgcggcgagg
     3541 gctgcaaggt gacggtggtc ccggcgcaga ctctggcgga agacgtgctt gctctgaagc
     3601 ccgacggagt tttcttgtcc aacgggcctg gtgatccgga gccgtgcacg tatgccgtcg
     3661 attcgatccg ccgcctgatg ggacggcagc cgatttttgg aatctgcctc ggacatcagc
     3721 tgacgggact cgcgctggga gggagaacct tcaagctaaa gttcggccac cacggcggga
     3781 atcatccggt gaagcagctg aagaccggta agatcgagat cacggcgcac aaccacaact
     3841 ttgccgtcga tcccgattcg ctagccgaaa gtgaagtcga gctgacgcac atggatctga
     3901 acgatcagac actggaaggc ctgcgccatc gcaacctccc gctgttcagc gtccagtatc
     3961 acccggaagc cgcccccggc ccgcacgatt cgcactatct gtttaaggag ttcacgaaga
     4021 tgatggagga gtggaagggg tgaagcacgc aggaatggcg gccgtatgct ggctgcttct
     4081 tatcggattc tccaatgcgc aagctcttac gctttacccg gggtcggata aaaccacaaa
     4141 atgccccaga aacgtcgcga ttaacgtgat tgccgatggc aagccgctga aggacgtaaa
     4201 ggtggaattc tgccagactc cacagaacca aaactgtgtc tccggcacca ctggcgacga
     4261 tggcaaatca aagttcctgc aactgcggcc aggaaaccat tctctgcacg taacgaccaa
     4321 gaatggcata atcgcgaccg agttgaggtt tcacacctcg cacagcagca aagcaagtca
     4381 gttcacggtg gatatgactg gggagattcg ctttgccacg cagctgatag catacggcga
     4441 tggacttcct atagagagtc gccttcagcg gtttcaaggc ttcacgcaag accatgcggg
     4501 atcagccgtt ccagaagcat acatccgagt ggtacgcaaa gccgacaaac aaggcgaaaa
     4561 cccaatactt ctgtactctg accaggacgg gcacttctcg gctcacctgg aagatggatt
     4621 ttacatcgca ttttttcgaa gagtcggttt cgtcacacag acagttgcat ttgagatcag
     4681 cccgcaaggg g
//
LOCUS       DASPUL010000197         4398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_309978, whole genome shotgun sequence.
ACCESSION   DASPUL010000197 DASPUL010000000
VERSION     DASPUL010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309978"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..485)
                     /locus_tag="VEJ00_04590"
     CDS             complement(111..485)
                     /locus_tag="VEJ00_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXX00469.1"
                     /translation="MLEVILKRFEQPDEVRNFEKGKFELIHIGGMTIGRATYAPGWKW
                     SVHVGKALGKTSCDVEHVGMVLSGRATAAMDDGRILEMKAGDVFYIAPGHDSWVIGDE
                     PYVSLHLLGASDYAAHKPAARH"
     gene            711..2687
                     /locus_tag="VEJ00_04595"
     CDS             711..2687
                     /locus_tag="VEJ00_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXX00470.1"
                     /translation="MVDPTSIRASGLPQTSAETILSPTILEAIPDAVVAVNQQGIIVH
                     INSQTELLFAYTRDELIGQKIEMLVPERQRPQHHQHREHFHQQPKIRRMGSGLDLCGR
                     RRDGSEFPVEISLSPIAAGEGTVVLSVIRDISDRKRIEEELRRANDELDRRKSRELRD
                     SQNRLALIVDSSQDAIIGKNLDGIVTHWNKGAEHIYGYTAQEMIGQSISKLCPRDRSD
                     EIPAILQKIRNGERVEYFETVRVTRDGRHLSVSVAVSPIFDAEGKVVGASTIARNVTA
                     QKKVEDQLRQSQKMEAVGRLAGGLAHDFNNLLGIVTACTELLRNRIDPSNMEYIDNIR
                     EATKRGASLTRQLLGFSRKQPVQVQVLDLNERLKELIKLLHPLMGEDVEIALLPREAT
                     AIVEGDPSQLDQIVVNLAVNARDAMPNGGKLVVETAVFDFDESFAKEHPLMLAGRYVM
                     LAISDNGIGMDEATRSRIFEPFFTTKEIGKGSGLGLATVYGIVKQNGGHVWVYSEPAH
                     GTTFKIYLPCADHKLGIAPAAEAETLPARRDGTTILLVEDEPTMRRLTRKMLEEHGYR
                     VLEAEDGKAALEVIGSHRETIGLTLTDVVMKGMSGPEMVLRLIDSHPTMKIVYMSGYT
                     GELVANHGLDGTIRLLEKPFTRAALLKTLDATLG"
     gene            complement(2741..3538)
                     /locus_tag="VEJ00_04600"
     CDS             complement(2741..3538)
                     /locus_tag="VEJ00_04600"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXX00471.1"
                     /translation="MDFREISGHAGKLLSLEGKVAIVTGAASGIGRGIAIQLARMGAM
                     VFVLDKDRAGGEQAVSTIQNAGGDASFVSCDIRSVEECRDAVKRAVGARGRTDILCNN
                     VGVILRKDVVSMKEEEWAAVLDVTLTGIYRLSREVIPHMVRGGGGSIINTGSGWSLKG
                     GPRAAAYCAAKGGVLNLTRAMAIDHGKDNIRVNCICPGDIDTPMLASECRQLGADPEL
                     FMKEAANRPLKRIGKPEDVANAVLFLASDMARWITGACIVVDGGGLA"
     gene            complement(3563..>4398)
                     /locus_tag="VEJ00_04605"
     CDS             complement(3563..>4398)
                     /locus_tag="VEJ00_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXX00472.1"
                     /translation="PRLAGDAIKIADSAGLNPHALDDTIVVPWNDLPALQFAIEAHPG
                     QIAAVITEGVMANMGVIPPASGYLQALQKLTQQHGILFLLDETVTGFRIAPGGCQEYY
                     KLKPDLVSFGKALGCGLPVAAFAGRADVMSALAGGTVLHYGTHNASRIGMFAARASLQ
                     KLMRNGGEAFRYIWDVADQLAAGLTELFHRKGVAAIVQRVGPMFQIMFTERESIGDYR
                     EFCKFVDRKKYQRLSWKLFEFGVYTSPAATLHSIVTVAHTTTDVEKTLAAVDRALDAL
                     A"
BASE COUNT          929 a         1323 c         1273 g          873 t
ORIGIN      
        1 ttgcggcgca gtgtgataca cgaaacccat tgcaccttga gcctgcgcga gcgaccgggg
       61 agttggcctt tggaccgaac cctcagtcca gaagctccgt gccagcgctc ttagtgcctt
      121 gcagctggct tgtgagctgc gtagtcgcta gcgcccagca ggtgaagaga aacataaggc
      181 tcatcgccga tgacccagct gtcgtggccc ggtgcaatgt agaagacgtc tcccgccttc
      241 atctcaagga ttctgccgtc atccatcgca gcggtcgcac gtccggaaag aaccatgccc
      301 acatgttcca cgtcgcagct cgtctttccc agcgctttgc cgacgtggac agaccatttc
      361 caacctggtg cgtaagtggc gcggccgatg gtcattccgc caatgtggat cagctcgaac
      421 ttgccttttt caaaattgcg aacctcatcc ggctgctcga acctcttcaa aatcacctca
      481 agcatgatct cccccagggt tttctaggat gcgccgcggc agtgtcacga ttcaaccagg
      541 gggaacgatt ttcaggagtc ccaaatcggc catggtcctg tcagaagccc ctcagaggcg
      601 ggaaacagct ccttgcgccc tactcaagtt agggttaacc ttttcatatt tcgggggagc
      661 ggggccggta tctgccggga cccgtttatg tggtgattat gagccagccg gtggtcgatc
      721 caacctcgat ccgcgcgtcc ggactgcccc agacgagtgc tgagacgatc ctgtctccaa
      781 ccattctgga ggccattccc gacgcagtgg ttgcagtaaa ccagcagggg atcatcgtcc
      841 acatcaattc tcagaccgaa ctgctgtttg cctacacgcg cgacgaattg atcgggcaaa
      901 aaatcgagat gctggtgccg gagcggcagc gtccgcagca ccatcagcac cgcgagcact
      961 ttcaccagca gcccaagatt cgtcgtatgg gatcggggct ggacttgtgc ggcaggcgcc
     1021 gcgacggctc ggaattcccc gtcgagatca gtctgagccc gattgccgcg ggcgagggta
     1081 cggtggtgct gagcgtcatc cgcgacatca gcgatcgcaa gcgtatcgag gaagaactgc
     1141 gccgcgccaa cgacgagctc gaccggagaa agagcaggga actgcgtgac tctcagaacc
     1201 ggctcgcatt gatcgtcgac tcctcccagg atgcgattat cggcaagaat ctggatggca
     1261 tcgtcacaca ctggaacaag ggcgccgagc atatctacgg ctatacggcg caggaaatga
     1321 tcggtcagtc catttcgaag ctttgcccgc gagaccgtag cgacgaaatt cctgcgatcc
     1381 tgcaaaagat ccgcaacggc gagcgggtgg agtatttcga gaccgttcgc gtcaccagag
     1441 acggacggca tctcagcgtt tctgtcgcag tttcccccat ttttgacgcg gaagggaagg
     1501 tggtcggggc gtcaactatc gcccgcaacg ttactgccca aaaaaaggtc gaggatcaac
     1561 tgcgccagtc gcaaaaaatg gaggcggtgg gccggctcgc gggcggcctg gctcatgact
     1621 tcaacaacct tctggggata gttactgcct gcacggaact tctgcggaac cggatcgacc
     1681 caagcaacat ggaatatatc gacaatatcc gggaagccac gaaacgggga gcgtctctca
     1741 ctcgccaact gcttggcttc agccgcaagc agccggttca ggtccaggtg ctggacctga
     1801 acgagcgcct gaaggaactg atcaagctcc tccatcccct catgggagag gacgtcgaga
     1861 tcgcgctgct gccccgggaa gccacggcaa ttgtcgaagg ggaccccagc cagctggatc
     1921 agatcgtcgt caatctcgcg gtcaacgctc gcgacgccat gccgaacggc gggaagctgg
     1981 ttgtggagac cgcggtgttc gattttgacg agtctttcgc gaaggaacat ccgctcatgc
     2041 ttgccggccg gtatgtgatg ctggcgatca gcgacaacgg aatcggtatg gatgaggcca
     2101 cgcgctctcg catcttcgag cccttcttta ccaccaagga gatcgggaag ggaagcggcc
     2161 tggggcttgc caccgtctac ggcatcgtca agcagaacgg gggacatgtc tgggtctaca
     2221 gcgaaccggc tcacgggacg acattcaaaa tctatcttcc gtgtgccgat cacaagttgg
     2281 ggatcgcgcc ggcggctgaa gctgagacgc tacccgcacg acgcgacggc accacaatcc
     2341 tgctcgtcga agatgagccc accatgcgcc gtctgacgcg caagatgctc gaagagcacg
     2401 gctatcgggt tctcgaagcc gaagatggga aggcagccct ggaggtcata ggctcccatc
     2461 gtgagacgat cggcctaacc ctgaccgacg tggtcatgaa gggcatgagc ggacccgaga
     2521 tggtcttgcg cttgatcgac tcccacccca cgatgaagat cgtgtatatg tcgggataca
     2581 ccggggagtt ggtggcgaac catggattgg acggtacgat tcgcttgctg gagaagccgt
     2641 ttacccgcgc ggccctgctt aagacgctcg acgcgacgct gggatagttg tcagtcgtgt
     2701 ggtctcagta ttcagtcctt ccgctcgtta agcacggcga tcaagccagc ccacctccat
     2761 ccaccacgat gcacgcacca gtgatccacc tcgccatgtc gctcgctagg aataaaaccg
     2821 cgttagccac atcttcaggt ttgccgatgc gtttcagcgg gcgattcgcg gcctccttca
     2881 tgaacagttc gggatcggcg ccgagttgcc ggcactccga cgccagcatg ggcgtgtcga
     2941 tgtcgccggg gcatatgcaa ttaacgcgaa tgttatcttt gccatggtcg atcgccatcg
     3001 ctcgagtcag attcaatacg ccacccttgg ccgcgcagta agccgcagct cttggacctc
     3061 ctttcagcga ccatccggaa ccggtgttga tgatgctgcc gccaccacca cggaccatgt
     3121 gcggaatcac ctcccgcgac aaccgataga tgccggtcag cgtcacgtcg aggactgccg
     3181 cccattcttc ttccttcatt gacaccacat cttttcgaag gatcacgccg acgttgttgc
     3241 acaagatgtc ggttcgaccg cgtgcgccaa cggcgcgctt cacggcgtct cgacattcct
     3301 cgacagaacg aatgtcgcac gaaacaaagc tggcatcgcc gcccgcattc tggatcgtcg
     3361 agaccgcttg ctcaccgcct gcacggtctt tgtcgagtac gaaaaccatc gctcccattc
     3421 tcgccagttg gatcgcaatg ccgcgtccga tgccggaggc cgccccggtc acgatcgcga
     3481 ccttcccttc aagagaaagc agcttcccgg cgtggcctga gatttcgcgg aaatccattt
     3541 cgtcctcgtt gcagaggtct gcttacgcca gagcgtccag agctctgtcc accgcggcca
     3601 gcgttttctc tacgtcggtg gtagtgtgcg caactgtcac gatcgagtgc agcgtggctg
     3661 cagggctggt ataaacaccg aactcgaaca gcttccacga taagcgctgg tatttcttgc
     3721 ggtcgacgaa cttgcagaat tcgcgatagt cgccgatcga ctcccgctcg gtaaacatga
     3781 tctggaacat ggggccgacg cgctgaacga tggcagccac gcccttgcgg tgaaacaatt
     3841 ctgttaatcc tgcggcaagt tgatccgcca catcccagat gtagcggaat gcctcgcctc
     3901 cattgcgcat cagcttttga aggctggcac gcgccgcaaa cattccaatg cgggaggcat
     3961 tgtgcgtgcc gtagtgcagg acggttccgc cggccagtgc agacatcaca tcggcgcggc
     4021 cagcgaacgc agcaacggga agcccgcagc ccagagcttt tccgaaactg accagatcgg
     4081 gtttcagctt gtaatactcc tgacagccgc cgggcgcgat gcgaaatcct gtaacagtct
     4141 catcaaggag gaaaagaatg ccgtgctgtt gcgtgagttt ctgcagcgcc tggagatatc
     4201 ccgaagcggg agggatcacg cccatgtttg ccatcacgcc ctcagtgatc actgccgcga
     4261 tctgaccggg gtgggcttca atcgcaaact gcaaagcggg cagatcgttc cagggaacca
     4321 cgattgtgtc atcgagcgca tgtgggttga ggccggcact gtcggcgatc ttgatggcgt
     4381 cacccgccag acgcgggt
//
LOCUS       DASPUL010000198         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_443673, whole genome shotgun sequence.
ACCESSION   DASPUL010000198 DASPUL010000000
VERSION     DASPUL010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443673"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..562)
                     /locus_tag="VEJ00_04610"
     CDS             complement(212..562)
                     /locus_tag="VEJ00_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXX00473.1"
                     /translation="MAAPRRGTTGHGCYFITSSTFQKRNILQSDRMAGLFVEVLSHYR
                     EQGKYLLHEFVVMPDHFHLLITPNLTLERAIQLIKGGFSYRAKKELGFGHEIWQSSYY
                     DRRVRDVNEYFAFR"
     gene            complement(707..1366)
                     /locus_tag="VEJ00_04615"
     CDS             complement(707..1366)
                     /locus_tag="VEJ00_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00474.1"
                     /translation="MMDKSTVRIAALSLLALFAAAGGYSKHHASRQASQGKPGVFDYY
                     LLTLSWSPEFCHSPAHADKPECQSGHYGFVVHGLWPQFAEGYPENCSNAPGLANPAEM
                     ADIMPDAGLVAHEWSTHGTCSGLGAEAYFKELRQAFGLVKVPGRFEAPRESFSIPPQE
                     VKNEFVQANPNFRAEDMTVSCGNNYLTAVSFCLTKDLRPTACQNLRDCRANVIQVPPV
                     R"
     gene            1611..2216
                     /gene="pdxT"
                     /locus_tag="VEJ00_04620"
     CDS             1611..2216
                     /gene="pdxT"
                     /locus_tag="VEJ00_04620"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HXX00475.1"
                     /translation="MNIGVLALQGDFDAHRRRLEELGAKVVLVRKAEQFDQIDGLIIP
                     GGESSTFLKLLGEAGFEKLRQFVRAKPTFGTCAGAILLASEIENPPQAGLGAIDMRIR
                     RNAYGRQIDSSIREGTLVSRHRSGDPHDPALPESSLEMVFIRAPKIVHVGDDVEVLAT
                     EGRDPVAVRQGRVMAATFHPELSDDTRVHRAFLDLVRNGVG"
     gene            2255..2524
                     /locus_tag="VEJ00_04625"
     CDS             2255..2524
                     /locus_tag="VEJ00_04625"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HXX00476.1"
                     /translation="MCRNIKTLFNFDPPVTEEEVRAASLQFVRKISGFNKPSKTNEAA
                     FLAAVDEVAAVSSRLLLSLETTAAKRNREEEAARAKARALARFGT"
     gene            2618..3022
                     /locus_tag="VEJ00_04630"
     CDS             2618..3022
                     /locus_tag="VEJ00_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00477.1"
                     /translation="MPASYVIDKRRRLVVTTGSGCVTFAETKAHQDQLLSDPDFDPDF
                     DQLVDCTAVTELELSAEEARIIIGRRVFSPASRRAFVSANPAIFGMGRLMEAYNDMAP
                     APPQVRVFRDLSSAWRWLGLEEAASSGATSGR"
     gene            3033..3488
                     /locus_tag="VEJ00_04635"
     CDS             3033..3488
                     /locus_tag="VEJ00_04635"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXX00478.1"
                     /translation="MERVTVQLRIMRHRSVTYSPETTFQALADPTRRAVLDLLRRGSQ
                     PAGQIASAFPVSRPAISKHLRLLRRAHLVREHREGRNRVYQLNPEPLRAVDSWIDQYR
                     RFWSVNLASLKSFVEAEYANESQAAPAQPASRKQKRKPVSQSHQGELPQ"
BASE COUNT          724 a         1123 c         1029 g          691 t
ORIGIN      
        1 cgccacccaa gactagacac cctgcaagca cagcactttt cggcccactc aagcccggtt
       61 ttgacttgag tgaggagtcc caagctcatg cagcgctttg ccttctgggt ggcgcagcgc
      121 tttcagcgct gcgatttagg cgagctgagc tataaggaaa tgtctcgggg ctagccgcca
      181 agccgcgctt cacagcattc cgccgaatgt attaccggaa ggcgaaatat tcatttacat
      241 cacgaacgcg gcgatcgtag tagctcgact gccagatttc gtgcccaaac cccagttcct
      301 ttttggcccg ataagagaaa ccacctttga tgagctgtat ggctcgttcg agagtcagat
      361 tcggtgtgat caacaaatgg aagtggtccg gcatgacgac aaattcatgc agcagatact
      421 tcccctgttc ccggtagtga gacagaactt caacaaacaa gccagccatt cgatccgatt
      481 gaagaatgtt tctcttctgg aacgtcgagg aggtgatgaa gtagcaaccg tgtccggtgg
      541 tgcctcgacg gggagcggcc atggagtaca cctcagcggc tggaagccgc tcgcactctg
      601 gcaactttat cgcagcgccg gaggcgctgc gccacccctg gtaccttgaa tgttcagccg
      661 catacaaatt tgggaacggc acagcccaag agagcgccat gctcgcttac cttaccggcg
      721 gcacctggat cacgttcgct ctgcaatcgc gcaggttctg gcaggcggtc ggcctcaggt
      781 cttttgtcag gcagaaagac actgcggtca ggtagttgtt accgcagctc acggtcatat
      841 cctcagcgcg gaaattcggg ttggcctgaa cgaactcgtt cttcacttcc tgcggcggaa
      901 tggagaagct ctcccgcgga gcctcgaacc tgccgggcac cttgaccaac ccgaacgcct
      961 ggcgcagctc cttgaagtaa gcttcggcgc cgagtccgct gcaggttccg tgtgtgctcc
     1021 attcgtgagc gaccaggccg gcgtccggca tgatgtcggc catctccgcc ggattggcga
     1081 gccccggcgc gttcgaacaa ttctcgggat agccctccgc gaactgcggc cagagcccgt
     1141 gcaccacgaa gccgtagtgc cccgactggc actcaggctt gtcggcgtgc gccggtgagt
     1201 ggcagaactc cggcgaccag gaaagcgtca gcaggtagta atcgaacacg cccggcttcc
     1261 cctggctagc ctgccttgag gcgtggtgtt tcgagtatcc cccagccgcg gcgaacagcg
     1321 ccagcagcga caaggcggcg atccggacag tggatttgtc catcatgtgc gtctccgtgg
     1381 cccgagtatc atagctcggt ttgagctctg ccggagcagt cgtcacctga acactgtgct
     1441 ggacaccacg cgaatgcatg gctaagcagc ctcctcagcc atctctccct tggctgttcc
     1501 accctgcaaa ccgtgggcgc tagaattgct ctggaatcag cgagacggct gagcctacaa
     1561 ctcgccaact cctctgtgct cccggcgcac tctgtggtaa aggttttgtt atgaacatcg
     1621 gggttctggc cttgcagggc gatttcgatg cgcaccgccg ccgactcgaa gagctgggag
     1681 ccaaagttgt actcgtcaga aaggccgagc agttcgacca gatcgacggg ctcatcatcc
     1741 cgggaggcga atccagcacg tttctcaaac ttctgggtga ggcgggattc gaaaagctga
     1801 ggcagttcgt ccgcgcgaag cctactttcg gcacgtgtgc cggagcgatt cttctcgcct
     1861 cggaaattga gaacccgccc caggcgggac tcggcgccat cgatatgcgg atccgccgca
     1921 acgcctacgg acgccagatt gacagctcca tccgcgaagg cacgctcgtg tcgcgccacc
     1981 ggtcgggcga tcctcatgat ccagccctgc cggagtcctc gctcgaaatg gtcttcatcc
     2041 gggccccgaa gattgtccac gtcggcgatg acgtcgaagt cctggccaca gaaggcaggg
     2101 atcccgtggc ggtgcggcag ggaagagtga tggccgcaac cttccatccg gaactaagcg
     2161 acgacacgcg cgttcaccgg gcatttctgg atctggtacg aaatggcgta ggctagttgc
     2221 tggcggaagc tttcaggcct cgaggcaggc aaccatgtgc cgcaacatca aaacgctttt
     2281 taacttcgat cctccggtga ccgaggaaga agtacgcgcc gcttcactcc agttcgtgcg
     2341 caagatcagc ggcttcaaca aaccgtccaa gacgaatgaa gccgccttcc tcgcagccgt
     2401 cgacgaggtc gcggcggtct cctcgcgcct gctgctttcg cttgaaacca ccgccgccaa
     2461 gcggaacagg gaagaagagg ccgcaagagc caaggcccgc gcccttgccc gctttggcac
     2521 ctaaggcggc ctcgcagtgg agagcgcaga actacaggca atgctgtatg ctcggaaagc
     2581 ggtaagcgcg atactgtaac cgtggggggc aacgcccatg cccgccagct acgtcatcga
     2641 caagagacga cgccttgtag tcaccacagg ctcgggttgc gtcaccttcg cggagacgaa
     2701 agcccaccag gatcaactgc tgagtgaccc tgacttcgac ccggactttg atcagcttgt
     2761 cgactgcacg gccgtaaccg agctggagct gtcggctgag gaagcccgca tcatcatcgg
     2821 gcgaagagtc ttctcgcccg cctcgcggcg cgccttcgtg tcagccaatc cggccatctt
     2881 cggcatggga cggctcatgg aggcatacaa cgacatggcc cctgcgcccc cgcaggtgcg
     2941 ggtcttccgc gatctttcct ctgcctggag atggctcgga ctggaggagg cagcttcgtc
     3001 cggtgcgaca tctggaagat aatccttgac agatggaacg cgtaactgta cagttacgta
     3061 ttatgcgtca ccgttcggtt acatattctc ccgagaccac cttccaggcc ctcgccgatc
     3121 ccacccgccg ggcggtgctc gatcttctgc gccggggcag ccagcccgcc ggacagatcg
     3181 ccagcgcctt tccggtgtcg cggccagcga tttccaagca tcttcgcctg ttgcgccggg
     3241 cccacctggt ccgcgaacat cgcgagggcc gaaaccgggt ttaccagctc aacccggaac
     3301 cgctccgtgc cgtggactcc tggatcgacc agtaccgccg cttctggagc gtgaatctcg
     3361 ccagtctgaa atcttttgtc gaggccgagt acgccaacga atcgcaagcc gcgccggccc
     3421 agccggcctc ccgcaaacaa aaaagaaagc cagtatctca atctcaccaa ggagaacttc
     3481 cgcaatgaag aggaaagcta cccatgcact gctcgcactt ctttttctgg gcgctcttgg
     3541 cgccgccccg gtgttggctc aaaccga
//
LOCUS       DASPUL010000199         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_717157, whole genome shotgun sequence.
ACCESSION   DASPUL010000199 DASPUL010000000
VERSION     DASPUL010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717157"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VEJ00_04640"
     CDS             complement(<1..728)
                     /locus_tag="VEJ00_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00479.1"
                     /translation="MARIPAVRRCPKSTETEDCSMRMAVYNSAIPDVILNSAKLVRRS
                     AFSLSISLLVSVFILTLTAAATDWSIPEQQIARKIVAVTGPGTVELTVENRSSLGRRD
                     REIIQNGLRSALEGVGIRFAKADQAAATVTVSLSQNPTAYVWVAEIRQAAGASAVVVV
                     STPRAEGSVVAHDSVPLSLRKTPVYEQEDPILDVAVLEENAAPTHIAVLDPEKISLYR
                     QQGGKWQQEQALEIVHTRPWPRDMR"
     gene            complement(798..2120)
                     /locus_tag="VEJ00_04645"
     CDS             complement(798..2120)
                     /locus_tag="VEJ00_04645"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXX00480.1"
                     /translation="MLALKYLLVAGGLGMILAAVSILGYDLYRELLYRRAQATPGAAV
                     PPVPQWRWRTSLALALLAWGPILLALSIVVVPSGMAGVRVSQTSGTQPGTLYPGMHFV
                     TPLVEDVALFDTRDQIFTTGLEGDGKSAASDVSAKSQLLNVQAKEGLTLGLAITVRYR
                     LDPKRLDYIQGNLPRPVEKEIVPPVVASVWRELVPNYTVRDVFSAKREEVRQRAAGMI
                     TQKLAADGIVVKEVMLRDIQLPAEYAKGLETLLLKEQENDRMGVETELKQKEVRIAEL
                     EAEATKVQQIKQAEGEAQVHVLQAKGEADAMQYTLPLKEKQIQQSKLEAEARKQATIQ
                     NAEAEAQAKVIDSKAETERRKLLAEAEADRIRVTAAADAERLQSEAAILKQNPLLINK
                     IVAERLSDKLQIMMVPADGKFFFANDVLRSMNMANQASQTEQSEEQHR"
     gene            complement(2154..2321)
                     /locus_tag="VEJ00_04650"
     CDS             complement(2154..2321)
                     /locus_tag="VEJ00_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00481.1"
                     /translation="MQMAITFMAVVAGLVFSLAVALLVEELIFGQVFRLLFAPARVTP
                     QTVAMKSGRKG"
     gene            complement(2384..>2716)
                     /locus_tag="VEJ00_04655"
     CDS             complement(2384..>2716)
                     /locus_tag="VEJ00_04655"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXX00482.1"
                     /translation="SRVSNPTYYHPGTVIPTGKDNPVGTRWVGISQKGYGIHGTNVPK
                     SVGHAASHGCIRLRNRDMERLFAMVRVGDAVEIRGERDEQVAQIFGDPADDATVASAE
                     TAAAAGGQ"
BASE COUNT          475 a          906 c          741 g          594 t
ORIGIN      
        1 cgcatgtctc gcggccacgg ccgggtatga acgatttcga gtgcctgttc ctgctgccac
       61 ttcccgccct gctggcggta gagcgagatc ttttccggat cgaggactgc gatgtgcgtc
      121 ggcgccgcgt tttcttcgag aaccgcgacg tcaagaatgg gatcttcctg ctcgtaaacc
      181 ggagtcttgc gcaggctgag cggcaccgaa tcgtgcgcaa ccaccgaacc ctcggcccgc
      241 ggcgttgaga cgacgactac agccgacgcg cctgcggcct gccggatctc cgccacccac
      301 acgtaggcgg taggattctg ggaaagcgaa actgtgactg tggccgcagc ctggtccgct
      361 ttcgcgaagc ggatcccgac cccctccagt gcgctccgca agccattttg aatgatctcg
      421 cggtcgcgcc ttcccagcga cgagcggttc tccaccgtca gttccaccgt gccggggcct
      481 gtgaccgcga caatttttcg cgctatctgt tgttcaggga tactccaatc tgtagccgcg
      541 gcggtgaggg tcaatatgaa cacggaaacc agcagggata ttgacaggct gaatgcagag
      601 cgtcggacca gctttgccga gttcaaaatg acatccggga tggccgaatt ataaaccgcc
      661 atgcgcatgg aacagtcctc ggtctctgta ctctttggac accgccgcac cgccgggatc
      721 ctcgccatcg tccgctcccg aacgccctcc tgacgccagg ttttctggca cgtcaggagg
      781 gccgtctgca gcgcggctta cctgtgctgc tcctcactct gttccgtctg gctcgcctgg
      841 ttcgccatat tcatgctgcg cagcacgtcg ttggcgaaga agaacttgcc gtcagccgga
      901 accatcatga tctgcaattt gtcggagagc cgctcggcaa cgatcttgtt gatcaacagc
      961 ggattctgtt tcagaatcgc cgcctcgctc tgcaggcgct cggcatcggc ggcagccgtc
     1021 acccggatgc ggtcggcctc cgcttccgcc agcagcttgc gccgctccgt ctccgcctta
     1081 ctgtcgatca ccttggcttg cgcttcggct tccgcgttct gaatggtggc ttgcttccgg
     1141 gcttctgcct ccagcttgct ttgctgaatc tgcttttcct tcagcggcag cgtgtactgc
     1201 atggcgtcgg cttcgccctt ggcttgcagg acgtgtacct gcgcttcgcc ttcggcttgc
     1261 ttgatttgtt gcaccttcgt ggcctcagcc tcgagttccg cgatgcgtac ttctttctgt
     1321 ttcagctccg tctccactcc catgcggtcg ttctcctgtt ccttgaggag caaggtctcc
     1381 agtcccttgg cgtactccgc cgggagctga atgtcccgca gcatcacctc tttgacgacg
     1441 atcccatccg ccgccagctt ctgggtaatc atgcccgcgg cccgctgccg cacttcctca
     1501 cgcttggctg agaaaacgtc gcgcaccgtg tagttcggca ccagttcgcg ccacacactg
     1561 gccacaaccg gcggaacaat ttccttctcc accggtcgcg gcaggttacc ctgaatgtag
     1621 tccaggcgct tgggatcgag ccggtagcgc acagtaatcg ccagcccaag cgtgagccct
     1681 tctttggcct gaacattcaa caactgggat ttggcggata cgtcactggc ggcactcttg
     1741 ccatccccct cgagaccggt ggtaaaaatc tggtcccggg tgtcaaagag cgctacgtcc
     1801 tcgaccagcg gtgtcacgaa atgcatcccg ggatagagcg tccctggctg cgtgccgctg
     1861 gtttggctca ctcgtactcc cgccatcccg ctcgggacca caacaatact caacgccaga
     1921 aggatcggtc cccaggctag aagtgccagc gccagggaag tgcgccaccg ccattgcgga
     1981 accggcggca cagccgcgcc gggagtcgcc tgtgcccgcc ggtacaacag ttcccgatac
     2041 aggtcataac ccaaaatgct cacagccgcg agaatcatcc cgaggccacc cgcaaccaac
     2101 aaatacttca acgccagcat ggtcgttctc cttgtttgaa gttgttttga aactcagccc
     2161 ttccggccgg acttcatcgc aaccgtttgc ggtgtcactc tggccggcgc aaacagcagc
     2221 cggaacactt gcccgaaaat cagctcttcc accagcaatg ccaccgccag cgagaagacc
     2281 agtcctgcaa ctaccgccat gaatgtgatt gccatctgca tatgtcctcc tgtcacgtcc
     2341 tcctgattcc gatctctttt gttcaagctc tttccgagga actctattgc cctcccgcag
     2401 ctgcagccgt ttccgcggat gcgacggtcg cgtcgtctgc agggtcgccg aaaatctgcg
     2461 caacttgctc gtcgcgctca ccgcgaattt ccaccgcatc gcccactcgg accatggcaa
     2521 acagacgctc catgtcgcga ttgcgcagcc ggatgcagcc atgcgacgca gcatgtccta
     2581 ccgatttcgg gacgttggtg ccgtggatcc cgtaaccctt ctggctgatt cccacccagc
     2641 gcgtgccgac gggattgtcc ttgccggtcg ggatcaccgt gcccgggtgg tagtaagtgg
     2701 gattggaaac gcggct
//
LOCUS       DASPUL010000200         3921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1681317, whole genome shotgun sequence.
ACCESSION   DASPUL010000200 DASPUL010000000
VERSION     DASPUL010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681317"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..424
                     /locus_tag="VEJ00_04660"
     CDS             173..424
                     /locus_tag="VEJ00_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00483.1"
                     /translation="MTFVDPLADVEVNVPGVMAMVVAPLVAQVSVLLAPELIPAGLAV
                     KEVIAGIVALFGAALDEPQPDKPAQINRMRASAQRLPQK"
     gene            764..1288
                     /gene="bstA"
                     /locus_tag="VEJ00_04665"
     CDS             764..1288
                     /gene="bstA"
                     /locus_tag="VEJ00_04665"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HXX00484.1"
                     /translation="MTDPCYPVGKFTYSGPLTETQRQEAISAIAETPANLRAAVKGLS
                     DAQLDTPYREGGWTVRQVVHHVPDSHLNSYIRFKLALTEDEPTIKPYFEDRWADLADT
                     KATPIEVSLTLLESLHDRWVRLLRSLKPEDWKRAFRHPELGAMTLEKTLALYQWHGRH
                     HVAHITELRKQRGW"
     gene            1407..1721
                     /locus_tag="VEJ00_04670"
     CDS             1407..1721
                     /locus_tag="VEJ00_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00485.1"
                     /translation="MIARVWHGYTKPEHADGYEALLKPELLPGISKKKGYKGSYLLRR
                     NLDAEVEFITILLFDSLDDIRAAAGHADYQTAIIPEERRRYLARWDAKAAHYEVAAMH
                     GR"
     gene            complement(1933..2553)
                     /locus_tag="VEJ00_04675"
     CDS             complement(1933..2553)
                     /locus_tag="VEJ00_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HXX00486.1"
                     /translation="MTETPSNQPPRRRWWLRLSVLLVLAGVLWLAIVAVRVVHTAGLE
                     EIHPADVIVVFGAAEYSGHPSPVLRARLDHAFDLYEKHIAPVVITTGGAAADPTFSEG
                     GVGKEYLRKRGIPEDALIAETQSSDTAQSAQRVAVIMRANGLHSCVAVSDAYHVFRIR
                     KLLEHEGVGPVYVAPRADSRPHSLRQRSEAALREATSYVLWRLGIT"
     gene            complement(2564..3211)
                     /locus_tag="VEJ00_04680"
     CDS             complement(2564..3211)
                     /locus_tag="VEJ00_04680"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXX00487.1"
                     /translation="MRAEIVKISCDNPESSLIKYAADQIRSGEVLGMPTDTFYGLAAD
                     PFNLRAVDRVYDIKTRSRHKPLSLLIESVDQAEELAKSLPEEFYALARKFWPGPLTII
                     VKAGSRLPLKVTANTGNVALRVPNARIPLAVVTAAAIPITATSANLSGASECTSAEQV
                     REQLQGRINIIVDGGASPRDVASTIVDLSDETARWRIIREGAIPADQISTFFAQG"
     gene            3527..>3921
                     /locus_tag="VEJ00_04685"
     CDS             3527..>3921
                     /locus_tag="VEJ00_04685"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HXX00488.1"
                     /translation="MKPASESESRFEHRLRRVEGRHNALVKELRQAFARGERTEHGDC
                     AIEGVRIVEEAIRSGLQFRALFFRESARERAERLLPQIGSHVETLLLPDKLFDGAVPS
                     ETPQGVAALVRLKEFTLDDVLERMQVGPIV"
BASE COUNT          749 a         1091 c         1327 g          754 t
ORIGIN      
        1 acttgttgcc cagctcagcg tgctgctgga gcccgagaca atactggcag ggctggccgt
       61 gaaggagctg atcgtcggtt tgctggcggc gcttaccgta acggtgatgg tcgatttgat
      121 tgagcctgcg gagttggtgg cggtcaacgt gtaggtggtg gttgcggatg gggtgacttt
      181 cgtcgatccg ttggcggatg tggaggtgaa tgtccccggc gtgatggcga tggtcgtagc
      241 gccgctggtc gcccaggtca gcgtgctgct tgcaccggag ttgattccag caggactcgc
      301 ggtaaaggaa gtgattgccg gcatcgtggc tttgttcggc gcagcgctcg atgaaccgca
      361 accagacaaa ccagcacaaa tcaatagaat gagagccagc gcgcaaagac ttccccagaa
      421 ataagacgag cgatagcttc gccgattgcc gccaaacgaa ttggtcacgt ccattccccg
      481 cccctttaag ctcgaccgca atgccgagca gtcgacgcaa acttccctct attcagcgta
      541 ttgccgagtg gattgggctt gtagctctct gtggccttag cccttttgac cgggttcgca
      601 ggcttacaat cggttcgtgt ctcgtacttt cgatctagca ccatggtttc gtgcgcagaa
      661 tgagcagtcc acccccaaaa tggctttcct ggaacgcgga ctcttgttga accctttgcc
      721 gctcaacggc catctgtaga tcgattgatt ctctggagga gcaatgacag acccgtgcta
      781 tcccgtcggc aaattcacct acagcggacc tctcacggaa actcagcgac aagaggcgat
      841 cagcgccatc gcagaaacac cggccaatct gcgagcggcg gtgaaggggc tctcggatgc
      901 gcaactggac acgccgtatc gcgagggagg atggacggtg cggcaggtgg ttcaccacgt
      961 gcccgacagc catctaaatt cttatattcg cttcaagctg gcgctgaccg aggatgagcc
     1021 aacgatcaag ccgtatttcg aggatcgctg ggccgacttg gcggacacga aggcgacgcc
     1081 gatcgaggtg tccttgaccc tgctcgagtc gctgcacgat cggtgggtgc ggctgctgcg
     1141 gtcgttgaag cccgaggact ggaagcgggc gttccggcat ccggagctgg gcgcgatgac
     1201 tttggagaag accttggcgc tataccagtg gcatggccga catcatgtcg cgcatatcac
     1261 ggagctgcgc aagcagagag ggtggtaggt cgcggcgccc ggcagcacgg ctctgtcttg
     1321 tggctggcgg taagagattt tcaccgcgaa gttcgctaag aacagccgca ggacgcaaag
     1381 aaaaacgggg caggaggact tccattgtga tcgcgagagt gtggcacgga tatacgaagc
     1441 ctgagcatgc cgacggttat gaggcgctgt tgaagcccga gctactgccg gggatcagca
     1501 agaagaaagg ctacaaagga agctatctgt tgcgccggaa tctggacgcg gaagttgaat
     1561 tcatcacgat cctgctcttt gattcgctcg acgacattcg cgcggcggcg ggacatgcgg
     1621 attaccagac ggcgatcatt cctgaggagc ggcggagata tctggcgcgg tgggacgcga
     1681 aggcggcgca ttacgaggtg gcggcaatgc acggccggtg agacagccga tgtcggagtc
     1741 ggcccattcg tggcacgatc acaagggcta aagcccttgc atttcggttc cgtgacgcgg
     1801 ccctgaaggg ccgctcttcc acggcaaccc acgtcgtcgg tagcgaccca gttttgtcca
     1861 gtgcttatcc ggcgacgagg ggcggtcgtg gtccttgtct ctacggcccc tttaccacag
     1921 ccacgtcggt ggctaggtga tccccagacg ccaaagcacg tagctggttg cctcgcggag
     1981 cgcggcttcg gaacgctgtc tcaggctgtg agggcgggag tcggcgcgcg gagcgacgta
     2041 gacggggccg acgccttcgt gctcgagcag tttgcggatg cggaagacgt ggtaggcgtc
     2101 gctgaccgcg acgcagctat gcagtccgtt ggcgcgcatg atgacggcga cacgctgcgc
     2161 ggattgcgcg gtgtcggagc tttgggtttc ggcgatgagc gcgtcttccg ggatgccgcg
     2221 cttgcgcagg tactccttgc cgactccgcc ttcgctgaaa gttgggtcgg ctgctgcccc
     2281 gccggtcgtt atcactacag gagctatgtg cttttcgtaa agatcgaagg catggtcgag
     2341 cctcgcgcgc aggacgggtg agggatggcc ggaatattcg gcggctccga atacgacgat
     2401 gacatcagcc ggatggattt cttcgagacc ggcggtgtgg accacccgca cagctacaat
     2461 cgccagccac agcactccgg ccaacacaag caggaccgac aagcgaagcc accagcgccg
     2521 ccggggaggc tggttagagg gtgtctcggt cattcgcgcg ggttcatcct tgagcgaaga
     2581 acgtggaaat ctgatcggcc gggatggcgc cttcgcgaat gatgcgccaa cgcgcggtct
     2641 cgtcgctgag gtcgacgatc gtggaggcca cgtcgcgggg agacgcgccg ccgtcgacga
     2701 tgatgttgat gcggccctgg agctgttcgc gcacctgctc ggcgctggtg cactcggagg
     2761 cgccgctcag gttggcagag gtcgcggtga tggggatggc agcggccgta actacggcta
     2821 acggaatcct ggcgttgggc acgcgcaggg caacgttgcc ggtgttggcg gtgactttca
     2881 agggcaggcg cgagccggcc tttacgatga tggtcagcgg gccaggccag aacttgcgcg
     2941 ccagggcata gaactcctcg ggcagcgatt tggcgagttc ctcggcctga tcgacactct
     3001 cgatcagcag cgacagcggc ttgtgacgcg agcgggtctt gatgtcgtag acgcgatcga
     3061 cggcgcgcag gttgaaggga tcggcggcaa gtccgtagaa cgtgtcggta ggcatgccta
     3121 acacttcgcc ggagcggatc tggtcggcgg cgtatttgat cagcgatgat tcagggttgt
     3181 cacaactgat tttgacgatc tcggcgcgca cagcgccccc ttcctgaaag gccgcaggat
     3241 tttgttgatc tggctgcgaa acctaccatc atacggcaaa acacggcacg atgagggggt
     3301 atgatgcggg ggtgcagttc aggccgagct ttctctcagg cgtcattccg gcgctccgcg
     3361 ccttcgccag cggggagcga ggaatccggc gcgaacgaag acgcagcgga tccgcgcgag
     3421 atccctcgct ccgcccgaaa ggcggcttcg ctcgggatga cgccgtcgaa gtggaggaga
     3481 gtttgaatca gcctggggcc ttcaccagcg cattttccgt caaggcatga agccagcatc
     3541 agaatccgag tcgcgtttcg agcaccggct gcgccgggtt gaggggcgcc acaacgcgct
     3601 ggtgaaggaa ttgcgccagg catttgcgcg cggcgaacgc acggagcacg gagactgcgc
     3661 gatcgaaggc gtgcgcattg tagaagaggc gatccgcagc ggattgcaat ttcgcgccct
     3721 gttctttcgc gagtcggcgc gggagcgggc ggagcggttg ctgccgcaga ttggctccca
     3781 cgtcgaaact ttgctgctgc ccgacaagct gttcgacgga gccgtgccca gcgagacacc
     3841 gcagggcgtg gcggcgctgg tgcggttgaa ggaattcacg ctcgacgatg tgctcgagcg
     3901 catgcaagtc ggcccgatcg t
//
LOCUS       DASPUL010000201         6837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_991598, whole genome shotgun sequence.
ACCESSION   DASPUL010000201 DASPUL010000000
VERSION     DASPUL010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6837)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6837
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991598"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1600)
                     /locus_tag="VEJ00_04690"
     CDS             complement(116..1600)
                     /locus_tag="VEJ00_04690"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HXX00489.1"
                     /translation="MDVLAKLFEQHFHAPVERVQPVQGELGGSGRKIIRLSGGQVSAI
                     GILYGVREENAAFVEFSRHFRRHGLPVPEIYAEDLDHGAYLEEDLGDTSLFEFLSGSR
                     TGERISPLAVEAYRKVIATLPRFQIEAGRDLNYKFCYPRGSFDRQSISWDLNYFKYYF
                     LRLAGIPFSEQALEDDFTQLTDFLLGAERDYFLYRDFQSRNIMLRDGEPFFLDYQGGR
                     KGALQYDVASLLYDAKADLPPELRQQLLDHYLDTLARFVNLKRDAFLEHYYAYVYIRI
                     MQALGAYGFRGFYERKAHFLQSVPYALKNLRWLLHNVRLPIALPTLMDAFHSMLASEK
                     LQGLASEADNLVLRVFSFSFHRGLPRDDSGNGGGFIFDGRSLPNPGREERFKQLTGKD
                     APVIDYLNQQPSVHQFLASVMSLVDASVSNYQQRGFKNLMVSFGCTGGQHRSVYLAEQ
                     LANHLRGRNGIEVVVRHLGLESLGEYYHSQLGDRRVGKPGAGAS"
     gene            1826..2254
                     /locus_tag="VEJ00_04695"
     CDS             1826..2254
                     /locus_tag="VEJ00_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXX00490.1"
                     /translation="MPRARATILCIDDHWNGLIGRKLLLEQQGYRVLEAIDGLEGLSL
                     FATHLVDAVILDYQMPGINGSVIAVEMKRLKPQVPILLLSAYGPLPRRKLQSVDQFLS
                     KSQPPAVLLSTLQDLLDCRSKPFFYRWLSHWKSRNQVVTR"
     gene            2514..2924
                     /locus_tag="VEJ00_04700"
     CDS             2514..2924
                     /locus_tag="VEJ00_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00491.1"
                     /translation="MPRFPQPRSVRAPRLRLHETVSVILQLENGRNLPARLHRISITG
                     GLLELSTYLEERTRVDLTFQLGFAFVHPKGEMLFPARGGDLGYLQPFRITAFAEEERH
                     RLQIEIADQLKQSMAPAKVGPGSGARPRFFMESF"
     gene            3083..3253
                     /locus_tag="VEJ00_04705"
     CDS             3083..3253
                     /locus_tag="VEJ00_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00492.1"
                     /translation="MNWKSRFVVGVLTALAMMLLTYALPRGGWIRETALVQFPMTVGI
                     WAIFVLLFVKLK"
     gene            complement(3320..3396)
                     /locus_tag="VEJ00_04710"
     tRNA            complement(3320..3396)
                     /locus_tag="VEJ00_04710"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3360..3362),aa:Met,seq:cat)
     gene            3604..4323
                     /gene="kdsB"
                     /locus_tag="VEJ00_04715"
     CDS             3604..4323
                     /gene="kdsB"
                     /locus_tag="VEJ00_04715"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HXX00493.1"
                     /translation="MKAIAVIPARLASTRLPRKMLREIAGTPLIGAVYEAVRSSPLLA
                     DVIVATDSEEIMAVCRKHSWNAQMTSTAHRSGTERVHEISQRVAADIYINVQGDEPLV
                     RPEHMAALIRVMENPAAEVGTVMTPAAEIDIANPNAVKVVTDLNGRALYFSRAIIPFD
                     RDGLQPRYFKHLGLYAYRKAALDRFVALPESSLEKSERLEQLRFLENGISIFVGETPF
                     DSVGVDTEEDLQRVIEILKRR"
     gene            complement(4328..5275)
                     /gene="bla"
                     /locus_tag="VEJ00_04720"
     CDS             complement(4328..5275)
                     /gene="bla"
                     /locus_tag="VEJ00_04720"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HXX00494.1"
                     /translation="MAANPGRPSSGVNVALLKSWLFAFLLASASVPAAGQSATPACSQ
                     CSEWNLPQTPFRIFANTYYVGTHGLSSILITSDAGHVLIDGGLPQSAQQIEANIRSLG
                     FRVEDVKIILNSHVHFDHAGGIAELQRRSGARVMASKWSAAVMRHGGVGRGDPQYGAV
                     TGIAPIKNVHEVSDSETLQVGAIAITAHLTPGHTPGGTSWTWRSCEGNFCRDIVYADS
                     LTPVSAGGFRFTSNREYPHALDDFEKSFVFLENTPCDVLITPHPEASDLWDHLAAREK
                     GGTPDPVATPGACRELAEHGRERLHQRIAEERSQPTGKR"
     gene            complement(5239..6396)
                     /locus_tag="VEJ00_04725"
     CDS             complement(5239..6396)
                     /locus_tag="VEJ00_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXX00495.1"
                     /translation="MTDDPTPVQADSPEAQRYNRIRRWLGIADFVVGFAFLVVLLVTG
                     WSSTLRDLAIRVGSQMYGLQLFVYVVLLLVISKALGVGFEYYGFRLERGFKLSNQRFR
                     SWAWDEVKGFLVGMVLATVVVELLYYTIREWPQHWWMLAWVLFMGLFILLAQLAPVLL
                     FPIFYKFEPLDNEDLRRRLVVLSERAGTRVRGVYRWKLSEKSKKANAALTGLGATRRI
                     ILADTLLDNYSPDEIEAVLAHELGHHVHRHILKSIFVQAAITFVGFWAANWALHFAIE
                     RHLFDFDGLHDFANLPLLALVSVVLSFLLMPALNAYSRYNERQADRYAFESIASLEPF
                     ISSMNKLAEQNLAERTPSKWVEWYFHSHPSVSKRLAAAAEWQRTQGAHPQV"
     gene            complement(6490..>6837)
                     /locus_tag="VEJ00_04730"
     CDS             complement(6490..>6837)
                     /locus_tag="VEJ00_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00496.1"
                     /translation="KPLGQLVADLQREYGSHYYGRRDLHIPEDVKQNAIQRARADSTQ
                     ALGRYRVLKKEHLDGVKFFLDAPTNGNGAEAWVLFRASGTEPLLRLYTEAASPDLVNE
                     LLGTGESFVRNNG"
BASE COUNT         1405 a         2029 c         2050 g         1353 t
ORIGIN      
        1 cggcgcaagg taatctccag gagcgtgtgt ccggcaactt ccaccagagc tttgggacga
       61 tcgtcggtaa gcggacgcag ccgcgtgccc aggccggcgg cgagaatcat ggccttcatg
      121 aagcgcccgc tccgggtttc cctactcgcc ggtctccgag ttgggaatgg taatactcac
      181 ccaaactctc caagcccagg tgccgcacga caacctcaat gccgtttctg ccacgcaggt
      241 ggttcgccag ttgctcggcc agatacaccg agcggtgctg cccgccggtg cagccgaacg
      301 acaccatcag attcttgaat ccgcgttgct gatagttgct gacgctggcg tcgaccagcg
      361 acatcacgct ggccagaaac tgatgcacgc tcggctgctg gttgaggtaa tcgatgaccg
      421 gggcgtcttt gccggtcagt tgtttgaagc gctcttcgcg gccgggattg ggcaggctgc
      481 ggccgtcgaa gatgaatccg ccgccgttgc cgctgtcgtc cctgggcagg ccgcggtgaa
      541 aggaaaagct gaaaacccgc agcaccaggt tgtcggcttc ggaagccagg ccttgcagtt
      601 tttccgaggc cagcatgctg tggaatgcgt ccatcagcgt cggcagcgcg atgggcagcc
      661 gtacgttgtg cagcagccag cgcaggtttt tcagcgcgta gggaacgctc tgaagaaagt
      721 gcgccttgcg ctcgtagaat ccacgaaatc cgtacgcgcc cagcgcctgc atgatgcgga
      781 tatagacata tgcgtagtag tgctccagaa acgcgtcgcg cttcaaattt acaaaacgag
      841 cgagcgtgtc gagatagtga tcgagcaact gctgccgcag ttccggaggc agatcggcct
      901 tggcgtcata caaaagagag gcaacatcat attgcagcgc gcctttgcgt ccgccctgat
      961 agtcgaggaa aaatggctcg ccgtcgcgca gcatgatgtt acgcgactgg aaatcgcgat
     1021 agagaaagta atcgcgctca gcgcccaaca ggaagtccgt cagctgcgtg aagtcgtcct
     1081 ccagcgcctg ctcgctgaag ggaatgcccg cgagccgcag aaagtagtac ttgaaatagt
     1141 tcaggtccca ggagatggac tggcgatcga aacttccgcg cgggtagcag aacttgtagt
     1201 tcaggtcgcg gccggcttcg atctgaaaac ggggcagcgt ggcgatgact ttgcgataag
     1261 cctcgactgc cagagggctg atgcgctcgc cggtgcggct cccggaaaga aactcgaaca
     1321 gcgaggtgtc gcccagatct tcttcgagat atgcgccgtg atcgaggtcc tcggcgtaga
     1381 tttccggaac cggcaggcca tgccgccgga aatggcgcga gaattcgacg aacgcggcgt
     1441 tttcctcgcg cacaccatac agaattccga tggcgctgac ctggcctccg gacaggcgaa
     1501 tgatcttgcg tcccgagccg ccgagttctc cctgcaccgg ctgcactcgc tcgactggcg
     1561 catggaaatg ctgctcgaaa agtttggcga gaacgtccat gaacagttga atgattcagt
     1621 gcgattgcac ccacaaagat accacccgca ccgttccaga tggggtaacc gccgcttgaa
     1681 tgcacgttcg taatttgtac gtgaagatga ttgcattcag aatcagcctg cccaccactt
     1741 cgaagttgag catcgggcag cctcgatcga agcaggctcc cgctgacagg aagcaaactc
     1801 tggccggatc ggaacccaag cggctatgcc tcgcgcaaga gcgacgattc tctgcatcga
     1861 cgatcactgg aatgggctca ttggccgcaa gctgctgctc gaacaacagg gatatcgggt
     1921 gctggaagca atcgatggcc ttgaagggct gagtctgttt gctacacacc tggtggatgc
     1981 cgtgattctc gattaccaga tgccggggat caacggttcg gtgattgcgg tggagatgaa
     2041 gcgcctgaaa ccgcaggtcc cgatcttgct gctctccgcg tatggaccgt tacccaggcg
     2101 caagctgcaa tcggtggacc agttcttgtc gaagtcccag cctcctgcgg ttctgttgtc
     2161 caccctgcag gaccttttgg actgccggtc aaaaccattc ttctaccgct ggctcagtca
     2221 ctggaagagc cggaatcagg tggtgacacg atgaactttc tggaaaaact cctcgggtgc
     2281 ggctgctcgc accggtttgc ctggccgcgt attgatgacg aagggcggca ctatcagatc
     2341 tgtctcatgt gcgggaccgc ttacgaatac gactggaggc agatgcggca gacggggcgg
     2401 ctgctggctg gagcgggggt aatgtacccg gggacccttt cttctcaggc ggaacacgct
     2461 ctttcgtcat ctcgcgctgc gctggggcgc taaataaaat cgttggaccg ctgatgccgc
     2521 gctttcccca accgcgctct gtacgcgctc cccgattgcg gctgcacgaa actgtttccg
     2581 tcatccttca gctcgaaaac ggccgcaacc ttcccgcccg gctgcaccga atctccatta
     2641 ccggtggctt gctcgagctc tccacctacc tggaagagcg cacccgcgtg gatctcactt
     2701 tccagctggg ttttgccttt gtccatccca agggcgaaat gctgtttcct gcgcggggtg
     2761 gcgatttggg ctacttgcag ccattccgga tcaccgcatt cgctgaagaa gaacgccacc
     2821 ggttgcagat cgaaatcgcc gatcagctga agcaatccat ggcgcccgcc aaagtcggcc
     2881 ccggatcagg cgcccgtccc cggtttttca tggagtcgtt ttagactccc cgccgaccga
     2941 accactcgac tgactctcgc tttagtgggc cgccgcaccc taaatcggtt tgtgcttaaa
     3001 ttacccggat cgggcatcaa acggctcaag taggtgtaca atcgccgcct gtcggtaaac
     3061 aggaaagcga ggtcgaaccg gaatgaactg gaaaagcaga ttcgttgtcg gcgtgctcac
     3121 cgctctcgcc atgatgctac tgacgtacgc tctgccccgc ggaggctgga tccgcgagac
     3181 ggccctggtg cagtttccca tgaccgtagg aatctgggcg atctttgtgc tgctgttcgt
     3241 gaaactgaaa tgatcgcctg ccgcggccgg gagcccactg gcgaaatccc gagcgcagcg
     3301 aggcaccttg cgtgaaatct ggtggcggcg gttggactcg aaccaacgac ctacggatta
     3361 tgagaccgtc gctctaacca cctgagctac accgccacgg tgtgaatcgg ggagaatgta
     3421 cgaaaggact tgggcaccgg cgtcgagcac ggcactcctt cgcacaaaga agcagcgccc
     3481 cgcggacgct gcctccaatt tcaattttag ggtccgctgc caaataggtc aaacctggcg
     3541 tgaccgcctg cacgctggcg actccgaccg cacgtagcct tgatttccaa tacactgacc
     3601 tgcatgaaag cgatcgctgt cattcccgcc cgtttggctt ccacgcgcct gccccgaaag
     3661 atgctgcgcg aaattgccgg aacgcctctg attggagcgg tgtacgaagc ggtgcgctcc
     3721 tcgccgctgc tcgccgacgt gatcgtggct acggactccg aagagatcat ggccgtatgc
     3781 cggaaacata gctggaatgc gcagatgact tcgacggctc atcgcagtgg cacggagcgg
     3841 gttcacgaga tctcgcaacg tgtggcagcc gatatctata tcaacgtcca gggggatgag
     3901 ccgctggtgc ggcccgagca catggcggca ttgatccgcg tgatggaaaa tccggcggcg
     3961 gaggtgggca cggtcatgac tccggcggca gagatcgaca ttgccaatcc gaatgcggtt
     4021 aaagtggtca ccgatcttaa cgggcgtgct ctgtattttt cccgtgccat cattcccttc
     4081 gatcgcgacg gcctgcagcc acgctatttc aagcacctgg ggttgtacgc ctatcgcaaa
     4141 gccgcgctcg atcgctttgt ggcgctgccg gagtcgtcgc ttgaaaagag cgagcggctg
     4201 gagcagttgc gctttctaga gaacggcatt tcgatctttg tgggcgagac cccttttgac
     4261 agcgtgggcg tggataccga ggaagatctg cagcgggtaa tcgagattct caagcggcgc
     4321 tgagggacta gcgcttgcct gttggctggc tgcgctcctc tgcgatccgc tggtggagtc
     4381 gctcgcgccc gtgttcggcc agctcccggc aggcgcccgg ggttgctacc ggatctggag
     4441 tacccccctt ttcccgcgcc gcaagatggt cccacagatc tgacgcttca ggatgcggcg
     4501 tgatcagaac gtcgcagggc gtgttctcca gaaagacgaa actcttttca aaatcgtcga
     4561 gcgcgtgcgg gtattcacgg ttgctggtaa accgaaatcc accggcggaa acgggggtca
     4621 ggctgtcggc atagacgatg tcccggcaga agttcccctc gcaagacctc caggtccagc
     4681 tcgtgccgcc gggcgtatgc ccgggagtca aatgcgccgt gatcgcgatg gcgccgacct
     4741 ggagcgtctc gctgtctgac acttcatgca cgttcttgat gggagcgatt ccggtaacgg
     4801 ctccgtactg cggatcgccc cggcccacgc cgccatgcct catgacggca gcgctccatt
     4861 tgctggccat cactctggcg ccgctccgcc gctgcagttc ggcaattccg ccggcgtggt
     4921 cgaaatgcac gtgcgaattc aggatgatct tcacgtcttc aacgcggaag cccagcgacc
     4981 gtatattggc ctcgatttgc tgagccgatt gcggcaagcc gccgtcgatc aggacatgcc
     5041 cggcatccga cgtgatcaga atcgaactca agccgtgagt cccgacgtaa tacgtgttag
     5101 cgaagatgcg gaagggcgtt tgtgggaggt tccattcgga gcactgactg caagcgggag
     5161 tcgcgctctg gcccgccgct gggacggatg cggatgctaa cagaaaagcg aagagccagc
     5221 tcttgagaag tgccacgttc acacctgagg atgggcgccc tgggttcgct gccattctgc
     5281 cgccgcggcc agccgtttcg atacggaggg gtgggaatgg aaataccact cgacccactt
     5341 tgatggagtg cgctcggcga ggttctgctc cgccagcttg ttcatggagg aaataaatgg
     5401 ctcaaggctg gcgatggatt cgaaagcata gcgatccgcc tgacgctcgt tgtaacgcga
     5461 gtaagcgttg agcgcaggca tgagcagaaa tgaaagtacg accgaaacca gcgccagcag
     5521 cggcaggttg gcgaagtcat ggaggccgtc gaagtcgaac aggtggcgct cgatcgcgaa
     5581 gtgcagggcc cagttcgccg cccagaagcc gacaaaggtg attgccgcct gcacgaaaat
     5641 gcttttcagg atgtggcggt gcacgtggtg cccgagctca tgggccagca ccgcttcgat
     5701 ctcgtccggg gagtagttgt cgagcagcgt atccgccaga atgatgcgcc gcgtcgctcc
     5761 cagcccggtc agagcggcgt tggccttctt gcttttttcc gaaagcttcc agcggtacac
     5821 gccgcgcacg cgcgtgccgg cgcgctcgct caagaccacc aggcggcgcc gcaggtcctc
     5881 gttgtccagc ggctcgaact tgtaaaagat ggggaagagc agcaccggcg ccagctgcgc
     5941 cagcagaatg aacaggccca taaacagaac ccaggccagc atccaccagt gctgcggcca
     6001 ttcccgaatg gtgtagtaga gcagttccac cacgacggtg gccaggacca tgcccaccag
     6061 aaaacccttc acttcatccc aggcccagga acgaaatctc tggttggaaa gcttgaatcc
     6121 gcgttccaga cggaacccgt aatactcaaa accgacgccc agcgctttgc tgatgacgag
     6181 cagaagcacg acatagacga agagctgcag gccgtacatt tgcgatccaa cgcggatcgc
     6241 gaggtcgcgc agcgtgctcg accacccggt gaccagcagc accaccagaa acgcgaagcc
     6301 gacgacaaaa tccgcaatgc ccagccatcg ccggatgcgg ttgtatcgct gtgcttcagg
     6361 cgaatcggcc tgaaccgggg tcggatcgtc tgtcatgagt ccagaaagaa gccctactta
     6421 gcagtatcgc attattgcca gcaggaactg gcgggattat tgcagattcc ggattacgca
     6481 ggtcagcgcc tagccattgt tcctcacgaa gctctctccc gtccccagca gctcgttcac
     6541 cagatctggc gatgccgctt cggtgtagag gcgcaacaac ggttccgttc ccgatgcgcg
     6601 gaacagcacc cacgcttcgg ctccattccc gttggtgggg gcatcaagaa agaatttcac
     6661 accgtcgaga tgttcttttt tgaggactcg ataacggccc agagcctgcg tggagtcagc
     6721 tcgcgcccgt tgaatcgcat tctgcttcac atcctcggga atatgcaggt cgcgccgtcc
     6781 gtagtagtgc gagccatact cacgctgcag atcggcaacc agttggccca gcggctt
//
LOCUS       DASPUL010000202         3991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_584850, whole genome shotgun sequence.
ACCESSION   DASPUL010000202 DASPUL010000000
VERSION     DASPUL010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584850"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="fabD"
                     /locus_tag="VEJ00_04735"
     CDS             complement(<1..842)
                     /gene="fabD"
                     /locus_tag="VEJ00_04735"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HXX00497.1"
                     /translation="MTDNAIAFLFPGQGSQAVGMGKDLAEKYPIARQTFEEADEALGY
                     KLSQVCFEGPEDQLRLTEITQPAILTASIAALRVLEGRAPKPSFVAGHSLGEYSAHVA
                     SGTISFADAVRTVRNRGKYMQEAVPVGVGAMAAILGMHLDKVIAVCHDAAQGEVCDAA
                     NINSPEQIVISGNSAAVERAAKLADERGAKRAKVLPVSAPFHCSLMKPAQDRLEVDLN
                     ALRMQKPVYAVACNVDAELVSDDLRARDTLVRQVTGSVRWEQCMHLLIGKGVQTFIEV
                     GPGKV"
     gene            complement(1009..2076)
                     /gene="plsX"
                     /locus_tag="VEJ00_04740"
     CDS             complement(1009..2076)
                     /gene="plsX"
                     /locus_tag="VEJ00_04740"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HXX00498.1"
                     /translation="MPSVIALDAMGSDRAPRPEIEGAILASRQYGTRVLLVGPETVVK
                     AELDRHRSAARLPISIVHASEYITMEDKVEAIRAKRDSSMRVGLRMVREGQAAGFVTA
                     GNTGAAMATAKVVLGAVPGVDRPALAAVFPTAPGTAAMLLDVGANVDCKAENLEQFAV
                     MGDVYFRAMFGQGMFGQGKRPGSLGPRVGLLSIGEEEGKGNDLTREAFQLLKQLPLNF
                     VGNVEGRDLYNGHVDVIVADGFVGNVALKISEGVANLVRTALKESLKATITRQVGALL
                     SRSAFEDFKKRLDHTEYGGAPLLGVKGVCIITHGSSNANAIKNAVRVAAEFAERGINQ
                     SIESGLAALRPNMAEEVTQPS"
     gene            complement(2325..2876)
                     /locus_tag="VEJ00_04745"
     CDS             complement(2325..2876)
                     /locus_tag="VEJ00_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HXX00499.1"
                     /translation="MFLDIKDLEVRPLEFQEDLQPGVIDFGGEVRQKTVLKTGGRAEL
                     VEEHHGKHQIIKDIRLRGRLTTGLELQCARCLDPVPQEITREFELLYRPLGADAGRDE
                     LSVTDAEAEIGYYQGEGMLLEDVLREQVLLALPLKITCREDCKGLCPHCGKNLNQEAC
                     SCNVPREDPRWAALKEVRTRLEH"
     gene            3045..3296
                     /locus_tag="VEJ00_04750"
     CDS             3045..3296
                     /locus_tag="VEJ00_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00500.1"
                     /translation="MATLYVENVPDDVYKALRKRAKANRSSIAAEVIALLQRNIPTAE
                     ELRERREFARRMREISSREPLSPGPFPSAEEMIREDRDR"
     gene            3293..3724
                     /locus_tag="VEJ00_04755"
     CDS             3293..3724
                     /locus_tag="VEJ00_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXX00501.1"
                     /translation="MTSFVLDASVAVKWMLPAKGELLRPQAFRLLDAYEAGDVDLIVP
                     DIFWAECGNVAWKAVRQQRLDRSSAELAIWSLTLRNIPTVSSAKLLQSAVSIALDFGR
                     SVYDSLYVALAAQSKTPLLTADERLANSLAARFPVKWLGAY"
     gene            complement(3753..>3991)
                     /locus_tag="VEJ00_04760"
     CDS             complement(3753..>3991)
                     /locus_tag="VEJ00_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00502.1"
                     /translation="AEWAKQRGVPVICNEFGVLRDYADPGDRAAWIRDVRTSLERHNV
                     GWAMWDYDDSFGVAAKKDGKAVLDENVVRALGLR"
BASE COUNT          723 a         1253 c         1201 g          814 t
ORIGIN      
        1 accttccccg gtccaacttc gatgaacgtc tgcacgcctt tcccgatcag caggtgcatg
       61 cactgctccc atcgtactga gccggtgacc tggcgaacga gcgtgtcgcg ggcgcgcaag
      121 tcgtctgaga ccagttcggc gtctacgttg caggcgacgg cgtagacggg cttctgcatc
      181 cttagcgcgt tgagatcgac ttccagccga tcctgtgctg gcttcatcag cgaacaatga
      241 aagggagcgc tcacgggcag caccttggcg cgcttggcgc cgcgctcgtc ggcaagcttg
      301 gcggcacgct caaccgcggc cgagttgccg gagatcacaa tctgctccgg agaattgatg
      361 ttggcggcgt cgcaaacctc gccctgcgcc gcgtcgtggc agacggcgat gactttgtcg
      421 agatgcatgc cgagaattgc ggccatggcg cccacgccca ccggcacggc ttcctgcatg
      481 tacttgccgc gattgcggac ggtgcgcacg gcatcggcga aactgatcgt gcccgaggcc
      541 acgtgcgcgg agtattctcc caggctgtga ccggcgacaa agctcggctt cggagctctt
      601 ccctctagca cgcgcagcgc cgcaatcgat gcggtgagga tcgccggttg cgtgatctcg
      661 gtcaggcgta gctggtcttc cggtccctcg aagcaaactt gcgagagctt gtagccgagg
      721 gcttcgtcag cctcttcaaa cgtctggcgg gcgatgggat acttctcggc gaggtccttc
      781 cccatgccga ccgcctgcga gccctggccg ggaaagagga atgcgattgc gttgtcagtc
      841 atgaattcga aaggcagttc gaccgcacga cttcaagtct ttcttggcga cctttgcggc
      901 agttcttcgc gatctttgcg atcaaaagct tttaccgcga agtgcgctga ggaacggccg
      961 caaagttcgc taagaacttc gcatcgatcc acgcatcggc ttcatcggct agctgggctg
     1021 cgtcacttct tcggccatgt taggccgcaa cgcggccagg ccgctctcga tcgactgatt
     1081 gatgccgcgc tcggcaaact cggccgcgac gcgcaccgcg ttcttgatgg cgttggcgtt
     1141 cgaggatccg tgcgtgatga tgcacacgcc cttcacgccc agcagaggcg cgccgccgta
     1201 ttcggtgtga tcgaggcgtt tcttgaaatc ttcaaaggcg ctgcgcgaga gtagcgcccc
     1261 cacctgacgg gtgatcgtag ccttcaacga ttctttcagc gcggtgcgca ccaggttggc
     1321 gactccttcg gaaatcttca gcgccacgtt gcccacgaag ccgtcggcga cgatcacgtc
     1381 cacgtgcccg ttgtacagat cgcggccttc cacgttgccg acaaaattca gaggaagctg
     1441 tttgagcagc tgaaaggcct cgcgtgtcag gtcgttgcct ttgccttcct cttcgccgat
     1501 cgaaagcagg cctacgcgcg gacccagcga tccgggccgc ttgccctggc cgaacatgcc
     1561 ttgtccaaac atggcccgaa agtagacatc gcccatgact gcgaactgtt cgagattctc
     1621 cgccttgcag tcgacgttgg cgccgacgtc gagcagcatc gccgcggtac cgggagccgt
     1681 cggaaagacc gccgccaatg cgggacggtc gacgccggga accgcgccca gcacgacctt
     1741 tgccgtggcc atagccgcgc cggtattgcc ggcggtgacg aatccggcgg cttggccctc
     1801 gcgcaccatg cgcagcccga cgcgcatgga ggagtcgcgc ttggcgcgaa tcgcctccac
     1861 cttgtcttcc atggtgatgt attcgctggc gtggacaatc gagataggca ggcgtgcggc
     1921 gctgcgatgg cggtccagtt cagccttgac cacggtctct ggaccgacca aaagcacccg
     1981 cgtgccgtac tggcgtgagg ccagtatcgc gccctcgatc tcgggcctgg gggcgcggtc
     2041 cgaacccatc gcatccagcg ctatcacgct aggcatggtg actagaagtt agctggcttc
     2101 ctcgacatcg agcacttcgc gccccttata gtacccgcac ttggggcagg cgcggtgagg
     2161 cattttgcgt tcgtggcagt tggggcattc agatagtgaa tggcctttaa gggcgtcatg
     2221 ggcccgccgc gttgaggtgc gcttctggga gtgtctccgt ttcggatttg gcatgttatt
     2281 cctctcgcgg agccctcggg ccggccgcgg aaattagtgt agtttcagtg ctcaagccgg
     2341 gtgcggactt ctttcaacgc cgcccagcgc gggtcttccc gcgggacgtt gcacgaacaa
     2401 gcttcctggt tcaaattctt gccgcaatgc gggcacaatc ccttgcagtc ttcgcggcag
     2461 gtgatcttca acggcaacgc cagcagcacc tgctcccgca ggacatcttc cagcagcatg
     2521 ccctcgccct gatagtagcc gatctcggcc tcggcgtcgg tcaccgacag ctcgtcgcgg
     2581 ccggcatcgg ctccgagcgg ccgataaagc agctcgaact cgcgcgtgat ctcttggggc
     2641 accgggtcaa ggcagcgcgc gcattgcagc tccaggccag tcgtcagcct tccccgcagg
     2701 cgtatgtcct tgatgatctg atgcttaccg tgatgctcct cgaccagttc agcgcgcccg
     2761 cccgttttga gaaccgtctt ttggcgaact tcgcccccaa aatcgatcac gcccggctgt
     2821 aaatcctctt gaaattccag aggacgaact tccaagtcct tgatgtcgag gaacatcgcg
     2881 aaacctccgc ggcaatgttg cccaagccgg cccgcggagc acgccaagag ggcgtaagta
     2941 ccgcaaggag caagacttag aataaagggc ggcgcgcaga agtgtcaaga aacggcacga
     3001 accatcggcc tatggatgtg ctaggatatc cctaggactc tcctatggct acactctacg
     3061 tcgagaacgt tccggacgat gtttacaaag cgctgcgcaa gcgggcgaag gccaatcgca
     3121 gctcgatcgc ggccgaggtc atcgcactgc tgcaacgaaa catccccacg gcggaggaac
     3181 taagagaaag gcgtgagttt gcacggcgga tgagggaaat ctcttcgcgt gaaccacttt
     3241 cacccggacc gttcccttcg gctgaggaaa tgattcgcga ggatcgcgac cggtgacctc
     3301 gttcgtccta gacgcgagtg ttgctgtcaa gtggatgctg cccgccaagg gagagcttct
     3361 caggccccag gcctttcgtc tgctggatgc ttatgaagct ggcgatgtgg acttgatcgt
     3421 gcctgacatc ttttgggcgg aatgcggtaa tgttgcctgg aaggcggtcc gacagcaaag
     3481 gctcgaccgc tcctcggccg aactggcgat ttggtcgctt actcttcgca atataccgac
     3541 tgtttcttcg gcaaagcttt tgcagagtgc cgtttcgata gctctcgact ttggccgctc
     3601 agtctatgac agtctctacg tcgcacttgc ggcccagagc aagacccccc tgcttaccgc
     3661 cgacgaacgc ctcgccaact ccctggccgc gcggtttccg gtgaaatggc tgggggcgta
     3721 ttgattggct tctcgggtac cgacagcagc gttcagcgca agccgagcgc ccttacgacg
     3781 ttttcgtcca gcacggcctt gccatctttc ttcgcagcca cgccgaagct gtcatcataa
     3841 tcccacatgg cccagccgac gttgtggcgc tcgagtgacg tgcgcacgtc gcggatccag
     3901 gcggcgcggt cgccggggtc ggcgtagtcg cgcagcacgc caaactcgtt gcagatgacc
     3961 ggaactccac gttgcttcgc ccactcggcc a
//
LOCUS       DASPUL010000203         3401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1265443, whole genome shotgun sequence.
ACCESSION   DASPUL010000203 DASPUL010000000
VERSION     DASPUL010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265443"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VEJ00_04765"
     CDS             complement(<1..447)
                     /locus_tag="VEJ00_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXX00503.1"
                     /translation="MFVRNSRVEPAISILVALATSVCFTDSVSAQTPAAAAKVVLPTD
                     RTVLPIPEPQYPHSTVFDVRNATPPPRFEVRAPADAPNVLIVLIDDMGFGQSSAFGGP
                     IHMPTVEALAANGLRYNEFHTTALCSPTRAALLTGRNHHMNNFGSIA"
     gene            1224..2675
                     /locus_tag="VEJ00_04770"
     CDS             1224..2675
                     /locus_tag="VEJ00_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase F"
                     /protein_id="HXX00504.1"
                     /translation="MVPPWTVAAGLLLCVANAMAADTTQEPPTPRALPTKVGAWKGDF
                     DGMVQRRLIRFLVPFSRTFYFNDKGRPHGLTADYAQDFERYINRKYAKELGKRPITVV
                     IIPITRDKLLRNVADGFGDIAAGDLTVTDERKKIVDFYVPEHEPALSEVVLTGPKSSP
                     IATADDLSGKTVHVRKSSSFYESLVALNDRFKKEGKPPAQLVPVPEALEDEDLMEMLN
                     AGLLQAIVVDDILAKPWAKVFPQIRVNEGAVLRTGGQLGWAIRKDSPKLGEALGDFYR
                     NAVWNIKLSATRTAQYARNIEQLKDPSKSADYKRFQDTIALFEKYGKEYEFDPLMLAA
                     QGYQESQLNQNAHSAVGAVGIMQVMPATGAELKVGDIHVAEANVHAGAKYMDQLMRRY
                     FPDAKFSEEDRTLFAFASYNAGAGSIARMRREAEKRGLDPNRWFNNVEIVTAEHIGSE
                     TTTYVRNIYKYYAAYKLMLEAQAEKERAHAVVH"
     gene            complement(2970..>3401)
                     /gene="msrA"
                     /locus_tag="VEJ00_04775"
     CDS             complement(2970..>3401)
                     /gene="msrA"
                     /locus_tag="VEJ00_04775"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXX00505.1"
                     /translation="GFTRNPSYQEVCSGDTGHAEVVRVIYDPRKIDYGQLLKVFWESH
                     DPTQGMRQGNDFGTQYRSAIYVSDPEQRAAAEESKRLYQARLTAAGRGTITTEIRDAP
                     PFYYAEDYHQQYLAKNPDGYCGIGGCGVPFKVAELQVGEKA"
BASE COUNT          718 a          990 c         1009 g          684 t
ORIGIN      
        1 cgcaatcgag ccgaagttgt tcatgtgatg gttgcggccg gtgagcagcg ccgcacgcgt
       61 cggcgagcac agcgcggtcg tgtggaactc gttgtagcgc aggccgttgg ctgccagggc
      121 ctctacggtc ggcatgtgaa tggggccacc aaaggcgctc gattggccaa atcccatgtc
      181 atcgatcaac acgatcagca cattgggtgc atcggcaggc gccctgacct cgaaacgcgg
      241 cggaggcgtg gcatttcgaa catcgaagac cgtgctgtgc gggtactgcg gttcggggat
      301 tggaagcacc gtgcggtcgg tgggcaatac gactttcgcg gcagcagcag gggtctgcgc
      361 actgaccgaa tctgtaaaac acactgaagt ggccagcgcg acgagtatcg agatcgcagg
      421 ctctactctt gaattacgta cgaacacggt gtccccctgg atgtttcggt taccagtctc
      481 gaaaaatcat gacttacgcc agcgatactc tcgcgttcac attttagacg gttatccatc
      541 gacggtcgct gcgaattccc gtgaaattgc ggcggtaccg gattctagat gccttgaggt
      601 gtgcgtcttc tctgaagtgg ccagccctcc aaagagaccc gtcgagaggt gacgcaaaat
      661 cgcttgcctc actggtaatt aactgggcga tcaacctcga tgctggccgc cttgctcgta
      721 tggcgcaagc acccgtgtcg acgagccagc gtgaagcgga gtaaccatgt tgaccgcgca
      781 taaggcaatg cagcctcgtc tggggcagct tgccctggct caagcagcga cccgacctta
      841 gggatcgatt cgccggccat atatcgtcgc cacagttctt cgcgttgcgc gttcgtaacg
      901 ctggagtccg tcccgcaccc atgtcgaaca tctcccaggc ttagattagc cgaggtgttt
      961 cgctgaccca ttgagaccgc ctgagtttat acgcgaattc ccgtattccg ctgattatga
     1021 gtgttgagca agctgactcg gaagcgccgc acgcaaatgt gggacagcga aaatgccatt
     1081 tgctcgagtt ggattccgat ccagagcaag cgtccgatcc cggtcatgcc catttacggc
     1141 ggggacgagc cgttctaggg caagttattc aagctgcccg atctggaatt agtgcagtga
     1201 atacctatgg gagaggaaga atcatggttc ctccgtggac cgtcgctgct ggcctgctgc
     1261 tatgcgttgc aaacgccatg gctgccgaca cgacccagga acctccgacg cctcgagcgt
     1321 tgccgacgaa ggtcggggca tggaagggtg acttcgatgg catggtgcag cgtcgactga
     1381 tccgttttct ggtgcccttc agtcgtacct tctatttcaa cgacaagggg aggccccacg
     1441 ggctgactgc cgattacgcc caggatttcg agcgctacat caatcgcaaa tatgcaaagg
     1501 aactcggcaa gcgcccgatc actgtcgtga tcattcccat aacccgcgac aagctactga
     1561 gaaacgtcgc ggatgggttt ggcgatatcg cggcaggcga tctgacggtc accgacgagc
     1621 gcaagaaaat cgttgacttc tacgttcccg agcatgagcc cgccctctcg gaagtggtcc
     1681 tcacgggacc gaagtcctcg ccgatcgcca ccgccgacga tttgtccgga aagactgttc
     1741 atgtgaggaa gtcctcgagc ttctacgaaa gcctggtggc gctgaatgat cgcttcaaga
     1801 aggagggaaa gcctccagcc caactcgttc ccgttccgga agcgctggag gatgaggacc
     1861 tgatggagat gctcaacgcc ggtctgttgc aggcaatcgt cgtcgacgac atcctggcca
     1921 agccgtgggc caaggtcttc ccgcagatcc gggtgaacga aggcgccgtc ctgcgtaccg
     1981 gtggccagct gggctgggca atccgcaagg acagtcccaa gttaggggaa gcgctaggtg
     2041 atttttaccg caacgcggtc tggaacataa agctttccgc cacccgcact gcccaatatg
     2101 ccaggaacat cgagcaactc aaggacccta gcaaatcggc cgattacaag cgcttccagg
     2161 atacgatcgc tcttttcgag aagtacggca aggagtacga gttcgatccg ttgatgctgg
     2221 cggctcaagg ctaccaggag tcgcagctga atcagaacgc gcacagcgcg gtcggcgccg
     2281 taggaatcat gcaagtgatg ccagccaccg gcgccgaact gaaggtcggc gacatccatg
     2341 tggccgaagc gaatgttcac gctggcgcga agtacatgga ccagctgatg agaagatact
     2401 ttcccgatgc gaagttcagc gaggaggacc gcacgctgtt tgcgtttgcc tcgtataacg
     2461 cgggggctgg gagtatcgcc cgtatgcgta gggaagccga gaagcgcggc ctcgatccga
     2521 accgctggtt caacaacgtc gagatcgtaa cggccgaaca catcggcagc gaaacgacga
     2581 cctacgtccg caatatctac aagtactacg ccgcctataa attgatgctc gaggcgcaag
     2641 ccgagaagga gcgggctcac gctgtcgtcc actaggcaat tcttggcgtc ggctggggtt
     2701 cagagcgcgt ggaaggatcg atgtcctctc tagagttctg cgaacgtccg ctcttcagca
     2761 agtagttgct acccagcggg tcgcaactgg tcagttctga ccccggccaa tcttcgcagc
     2821 ggattattct gatacgcggt cgacggtaga attccggtag acgtcagcgc cggacacggc
     2881 gcggattttc ccgcagaacc acgtatggcc ttggcttagg acgctgctaa cccgcggact
     2941 tcccgaagga accctctgtg aagctccgac tacgccttct cccccacctg taactccgcg
     3001 actttgaacg gcactccaca ccctccaatc ccgcaatacc catcgggatt cttcgccaga
     3061 tattgctggt gatagtcctc ggcgtagtag aacggcggcg catcgcggat ttccgtggtg
     3121 atcgtgccgc gcccggccgc agtgagccgg gcctgataga gacgcttcga ctcctcggcg
     3181 gcggcgcgct gctccggatc cgaaacgtag atcgcagagc ggtactgcgt accgaaatca
     3241 ttcccctggc gcatcccctg agtggggtcg tgcgattccc agaacacttt gagcagctgc
     3301 ccgtagtcga tcttgcgcgg atcgtagatg acgcgcacca cctcggcgtg cccggtgtcg
     3361 ccgctgcaga cctcctggta gctcggattg cgtgtgaagc c
//
LOCUS       DASPUL010000204         6861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_857070, whole genome shotgun sequence.
ACCESSION   DASPUL010000204 DASPUL010000000
VERSION     DASPUL010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6861
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857070"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VEJ00_04780"
     CDS             <1..273
                     /locus_tag="VEJ00_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00506.1"
                     /translation="GKLIDFGKQTEVPARDLVREYLEFIDDVVDELDSREELNYIHKI
                     VETGSGADRQLRVFQETGDLKKVVDYIIEETAAGLEEEPAAVRKVG"
     gene            333..1373
                     /locus_tag="VEJ00_04785"
     CDS             333..1373
                     /locus_tag="VEJ00_04785"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXX00507.1"
                     /translation="MGHPHSKTRLNELSFDPEYTPGARNAVNVCLRVQHGEQVCIITD
                     KATIEIAAAIAREVDKVGAPKRAWVLEELAERPLVDLPGEILDDLETAHVSIFAVQAQ
                     RNELRSRIQMTDVVNRRRIRHAHMVNINRQIMLEGMRADFRRVDRLSQKVVEMVRKAS
                     KIRAKTAAGTDLTADLNPNYKWLKTSGIISPEKWGNLPGGEIFTTPGEVNGTFVIDGV
                     VGDYLCAKFGNLEEYPLTVCMKANRLTEAHSENQELEDDFWRYTHTDANSDRVGEFAI
                     GTNIDLKNVIGQILQDEKYPGVHIAFGNPYGAHTGADWDSTTHIDVVGRKFNIWVDDQ
                     QIMQDGKFLVEA"
     gene            1435..2649
                     /locus_tag="VEJ00_04790"
     CDS             1435..2649
                     /locus_tag="VEJ00_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00508.1"
                     /translation="MISALRKQFNNNFTPEKYQKFLSRIDAICGTHVQFRLSETPCFF
                     PEELLDRMADDGRQLIRQLVDSPEYRARSNEAVPAEFKVPNEPDHPMFVQVDFGMVRD
                     AEGRIQPKLVELQAFPSLYAYQGPLAETYIEVYGLAAPGSGLPASGRLKYFLSGLEKN
                     SYHELLRRAIVGKQDPENVILMEIDPHHQKTFPDFALTGKMLGIRTVDITWIKKEGKR
                     LYYQLDGKRVPIKRIYNRTIVDELQRKNVKLNFDWRDDLEVEWAGHPNWYFRISKFSI
                     PYLKHESVPKTWFLDRLDAIPADLENYALKPLYSFAGLGVVIAPTKEDIAAIPPEKRP
                     YYILQERMHFEPVIETPFGGTKAEVRVMYIWLEELVPVLTIIRMGRGLQMGVDHNKNM
                     EWVGASAGLYVG"
     gene            complement(2678..3058)
                     /locus_tag="VEJ00_04795"
     CDS             complement(2678..3058)
                     /locus_tag="VEJ00_04795"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HXX00509.1"
                     /translation="MNALDSQAAHPWPTDPTDRREAIRRRAEEIYIRSGRIPGHDLEN
                     WMRAEQEILREAAETTARRKAIVVRVQGVEYVGEYDPASSDGYTPGELKKGMAVAVRF
                     SGDKMFVKRPDGRELETTVVRRTG"
     gene            3321..5720
                     /locus_tag="VEJ00_04800"
     CDS             3321..5720
                     /locus_tag="VEJ00_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXX00510.1"
                     /translation="MIGQQVSHYRILGKLGGGGMGVVYEAEDLKLGRHVALKFIPENL
                     AGDPKSLERFTREARSASQLNHPNICTIHGIEDNNGHPFIVMEKLDGESLKQHIAGHA
                     MEVEKVLEVGVQVAEALVASHAKGIVHRDIKPANIFLTPSGQVKVLDFGLAKLVHNVG
                     TDDDAGADNSLTAVGVIPGTAVYMSPEQARSETVDARSDLFSFGVVMYEMATGKKPFA
                     GANSLVTLDAVLHSKPVPPRQLNPKVPIELEGIIGKAMEKDRNHRYQSAAEMRSDIVL
                     LKRETESGTIKSGSNTARLRTVTHTFGHGSRVQTYLLLGMAGLLVTVLAAVGAWWFKH
                     REVANAEQRNAIAVLPLQNMNGDFNIDYLRFALADEVTSVLTYSRSLEVRPSSVTRRF
                     VALDLDPKKVGVELRVGRLLTGSFRRQGDQLMVTLEAIDVPSDRLLWQTTVVAKTDDM
                     VGLQEQLSSQVQHGLLPVLGGGGGAVEADASRPKNQQAYDFYLRSVAVPHDPKPNKEA
                     ITMLEWAVGIDPNYAPAWEALGLRYYYDSQYGGGGEAMFQRSNAAYERALALDPNRVM
                     AASSLITAHVERGELGPAYASAIDLVRRRPQSGDAHFALSYVLRYAGMLEQSAQECKE
                     ARTLDNGNYNFRSCAWTFVEMGKPDQAMDFVHLDPGSEFAAWVTPYVYLAGGNVAQAR
                     DAAKSMAKSPTYHRDLMEACTATQPPGDLAKIVRENEASVMTEPDAENWYRLGALMAA
                     CGQNEPALRLLKAAVQQNYCAYSALLDDPLLKNLRKETAFNQVLTAGSNCQAVLQQPS
                     Q"
     gene            6006..>6861
                     /locus_tag="VEJ00_04805"
     CDS             6006..>6861
                     /locus_tag="VEJ00_04805"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HXX00511.1"
                     /translation="MATASGERRPSSSTSARPAGTDVVADPPGLLYQGVPAEIPQDQG
                     AAGLHLELLRLGTTARLMQVVAHPDDEDGGMLTLEARGRGVSTLLMTMNRGEGGQNKV
                     GSNLSDVLGVLRALELLASDQYYGIQERFSHVADFGFSKSADETFAKWGGHDPALGDM
                     VRVIRTFRPDVLVARFSGTERDGHGNHQASSILAKEAFRAAADPRRFPEQIVEGLEPW
                     QVKKFYIGNVCGFGAMTCPDADWTLKLNSGERSTDLGMSYVQFAMEGLRHQLSQGAAN
                     WTVDPGDRF"
BASE COUNT         1423 a         1986 c         2164 g         1288 t
ORIGIN      
        1 ggcaagctga ttgacttcgg caagcagacc gaggttccgg cccgggattt ggtccgcgaa
       61 tacctggaat tcattgacga cgtggtggat gagctggact cgcgcgagga attgaactac
      121 atccacaaga tcgtcgagac cggcagcggc gccgaccggc aactgcgcgt cttccaggaa
      181 actggagatt tgaagaaagt tgtcgattac atcattgagg aaacggcagc gggactggaa
      241 gaagaaccgg cggccgtcag aaaagtaggg taatgaacac ttctggggag aaagtcaaaa
      301 ctcccacttc tcgcgcaaca agcgcgcgag aaatggggca ccctcactcg aagactcgcc
      361 tgaacgaact ttcatttgat ccggaataca cccccggtgc gcgaaatgcg gtgaatgtct
      421 gcctgcgcgt gcagcacggc gaacaggtgt gcatcatcac tgataaggct acgatcgaga
      481 tcgcggctgc gattgcgcgc gaagtagata aggtcggggc tccgaaacgt gcctgggtgc
      541 tggaggaact ggcggagcgg ccgctggtcg acctgcccgg ggagatcctc gatgacctgg
      601 agacagccca cgtctccatt ttcgccgtgc aggcgcagag aaacgaactg cgctcgcgca
      661 ttcaaatgac cgacgtggtg aaccggcgca ggatccggca cgcgcacatg gtcaacatca
      721 atcgccagat catgctggaa ggcatgcgcg cggattttcg gcgcgtcgac cggctgagcc
      781 agaaagtggt cgagatggtg cgcaaggcgt cgaagatccg cgcgaaaacc gcggctggaa
      841 ctgacctgac cgcggatttg aaccccaact ataagtggct gaagacgagc ggaattatct
      901 cgccggaaaa gtgggggaac ttgcccgggg gcgagatttt taccacgccc ggtgaagtaa
      961 acggaacgtt cgtgattgac ggggtggtgg gcgactacct gtgcgccaag ttcggcaatc
     1021 tggaggagta tcccctgacc gtgtgcatga aggccaaccg cctgacggaa gcgcactctg
     1081 agaatcagga actcgaggac gatttctggc gctacacgca caccgatgcc aacagcgatc
     1141 gcgtgggtga gttcgcgatc ggcacgaaca ttgatctcaa gaacgtgatc gggcagatcc
     1201 tgcaggatga gaagtatccc ggagtgcata tcgccttcgg caatccatac ggcgcccaca
     1261 cgggcgcaga ctgggactcg accacgcaca ttgacgtggt cggccgaaag ttcaacatct
     1321 gggtggatga ccagcagatc atgcaggatg ggaagttttt ggtagaagcg taaggccgac
     1381 ttgtctggag taacttcagg ttaaaataat cgccgcaggt tgaatccggc gattatgatt
     1441 tctgctctcc gtaagcaatt caacaacaac ttcactccag agaaatatca gaagtttctg
     1501 agtcggattg acgccatttg cgggacgcac gtccagtttc gcctgtccga gactccctgc
     1561 tttttccccg aagaacttct ggaccgcatg gctgatgacg gaaggcagct gattcgtcag
     1621 ctcgtcgata gtcctgaata tagagccagg tcaaacgagg ccgtgccggc agagttcaaa
     1681 gttcccaacg agccagacca tcccatgttc gtgcaagtcg atttcgggat ggtgcgggat
     1741 gcggagggcc gaatccagcc gaagctggtc gagttgcagg ccttcccttc cctatatgcg
     1801 taccagggcc cgctggccga gacgtacatc gaggtttacg gactggcggc tcccggctct
     1861 gggcttccgg cttccgggcg gctgaagtac tttttgagcg ggctcgagaa gaactcttat
     1921 cacgaactcc tgaggcgcgc gatcgtggga aagcaggatc ccgagaacgt gatcctgatg
     1981 gagatcgacc cgcatcatca gaagacattc cccgatttcg cgctgaccgg caagatgctg
     2041 ggcatccgca ctgtggacat cacctggatc aagaaagagg gcaaacgcct ctactatcag
     2101 ctcgacggca agcgtgtgcc catcaagcgc atctacaacc ggacaattgt cgatgaactg
     2161 cagcgcaaga acgtgaagct gaatttcgac tggcgggacg atttagaagt cgagtgggcg
     2221 ggccatccca actggtattt ccgcatcagc aagttctcga ttccctacct aaagcatgaa
     2281 tccgtgccca agacgtggtt tctggatcgg ctggatgcaa ttcccgccga tcttgagaac
     2341 tatgctttga agccgctcta ttccttcgcc gggctgggcg tggtgatcgc gccaacgaaa
     2401 gaagacatag ccgcgattcc gccggagaag cgtccttact acattttgca ggagcgcatg
     2461 cactttgagc cggtgatcga gactcccttc ggcgggacca aagctgaggt gcgcgtgatg
     2521 tacatctggc tggaagaact ggtgccggtc ctgacgatca tccgcatggg gcgcggcctg
     2581 cagatgggcg tggatcacaa caagaacatg gagtgggtgg gggcgtcggc gggattgtac
     2641 gtggggtagt gcgcgcgacc tggcacccag tgaacgctca ccccgtccgt cggacgacgg
     2701 tggtctccaa ctccctgccg tcggggcgtt tgacgaacat cttgtcgccg gagaagcgga
     2761 cagcaacggc catgcccttt ttcagctctc ccggcgtgta cccgtcagag gatgcgggat
     2821 cgtattcccc cacatattcc acgccttgaa ccctcaccac gatggccttc ctgcgcgctg
     2881 tcgtctcggc cgcttcgcgc aggatttcct gttccgcccg catccagttc tcaaggtcgt
     2941 gaccggggat tcggccgctg cggatgtaga tctcctcggc gcggcggcgt atagcctcac
     3001 gccgatcggt cggatcggtc ggccaggggt gggcagcctg cgaatcgagc gcgttcataa
     3061 gatgaggacc tccaaacccc tcgcgagcct cccattgcct atggtaatcg cgaaaaccgc
     3121 agaatgctcc gcggcatctc accaaatggg cgtagattgg ggccgctttg ggaagcgcca
     3181 cagacacgtt ccaaacggaa acccgtgctt gttgtgcagt ttaccctgcc ggccccccat
     3241 ccgtggccgc gcgagacaag gcagttgacc ggactctgcc gacagcctaa gatggccgcc
     3301 tacaggggaa ctgggcgctc atgatcggcc agcaagtatc ccattaccgc atccttggca
     3361 aattaggggg cggcggtatg ggtgttgtct acgaggccga ggatctcaaa ctcgggcgcc
     3421 acgtagccct aaagttcatc cccgagaatc tggccggcga tcccaaatcg ctggagcggt
     3481 tcacccgcga ggcgcggtcg gcctcgcagc tcaaccatcc gaatatctgc acgatccatg
     3541 gcatcgagga caacaacggc catcctttca tcgtcatgga gaagcttgac ggcgagagcc
     3601 tgaagcagca catcgccggg catgccatgg aggtcgagaa ggttcttgag gttggcgtgc
     3661 aggttgcgga ggccctggtg gcctctcatg ccaaaggaat cgtgcaccgc gacatcaagc
     3721 ccgccaacat cttcctgact ccgagcgggc aggtgaaggt gctcgacttc ggactcgcga
     3781 aactggtcca caacgtgggc accgacgatg acgcgggggc cgacaactcc ctgaccgcag
     3841 tgggggtgat tccgggcaca gcggtgtaca tgtctccgga gcaggcgcgc agcgaaaccg
     3901 tcgacgcgcg cagcgacctg ttttccttcg gcgtggtcat gtacgagatg gccaccggca
     3961 agaagccgtt tgccggggcc aactcgctgg tcacgctgga cgccgttctg cactccaagc
     4021 ccgtgccgcc ccggcagctg aacccgaagg ttccgatcga gctggaggga atcatcggca
     4081 aggcgatgga gaaggaccgc aatcatcgct accagagtgc ggccgagatg cgctcggata
     4141 ttgtcctgct caagcgcgaa accgagtccg gcaccatcaa gagcggcagt aacacggcgc
     4201 ggctgcggac cgtcacccac accttcggtc atggttcccg cgtgcagacc tacctgctcc
     4261 tgggcatggc gggactgctg gtcacggtgc tggctgcggt aggggcatgg tggttcaagc
     4321 atcgcgaagt ggcgaatgcg gagcagcgga acgccatcgc ggtgctgccg ctgcagaaca
     4381 tgaacggcga tttcaacatc gactacctgc gattcgcttt ggccgacgag gtgacgagcg
     4441 tcctgaccta ttcgcgatcg ctcgaggtgc ggccctcctc ggtgacgcgc aggtttgtgg
     4501 cgctcgatct tgatccgaag aaagtcggcg tcgaactgcg cgttgggcgc ctgttaacgg
     4561 ggtcgtttcg acgacagggc gatcagttga tggtcacgct ggaggccatt gacgttccaa
     4621 gcgaccgctt gctctggcag accacggtcg tcgccaaaac cgacgacatg gtggggttgc
     4681 aagagcagct cagttcgcag gtgcagcatg gattgctgcc ggttcttgga ggcggaggag
     4741 gagcagtcga agccgatgct tcccgcccca agaatcagca ggcttacgac ttctacctgc
     4801 gcagcgtggc cgtcccgcac gatcccaagc ccaacaagga agccatcacc atgctggaat
     4861 gggcggtggg catcgatccc aactacgcgc cggcgtggga agcgctgggg ttgcgctact
     4921 actacgattc gcaatacggc gggggcggcg aagcaatgtt ccaacgctcg aatgcggctt
     4981 acgagcgggc cctggcgctg gatcccaacc gtgtgatggc ggccagcagc ctgattacag
     5041 cccatgttga gcgcggagaa ctgggtccag cttatgccag cgccatcgac ctggtgcggc
     5101 gccgcccgca gagcggagac gctcacttcg cgctgagtta tgttctgcgc tacgcgggaa
     5161 tgctcgaaca gtcggctcag gaatgtaaag aagcgcggac gctcgataac gggaactaca
     5221 atttccgctc ctgcgcgtgg accttcgtgg agatgggcaa gcccgaccag gcgatggact
     5281 ttgtgcatct cgaccccggc tcggagtttg cggcgtgggt aactccgtat gtctatctgg
     5341 ccgggggcaa tgtggctcag gcgcgggacg ctgcgaagag catggccaag tctcccacgt
     5401 atcaccggga cttgatggag gcctgcacgg cgacgcagcc gcccggcgat ctggccaaga
     5461 tcgtgcgcga gaacgaagcg tcggtgatga cggaaccgga cgccgaaaac tggtatcgac
     5521 tcggagcgtt gatggcagcg tgcgggcaga atgaacccgc gctgcgcctg ctgaaggctg
     5581 cagtgcagca aaactactgc gcctattccg ccctcctcga tgatccactg ctgaaaaacc
     5641 tgcgtaaaga gaccgcattc aaccaggttc ttaccgcagg aagcaactgc caggctgtgc
     5701 tgcagcaacc cagccaatag gccgcggcgg actgggtccg cgcgcaggac tctggccggt
     5761 gacgaccgaa gtggtcccgg gctaacctga gcccttggct cgcgtctaat ccaagacagc
     5821 agcatacggc aaacaatttg cgtcaaaccg gggttaggcc ccatccttgt agcgtgagac
     5881 atcccgcacc tgccagcctg tcgcagcgcc gccgctgtgg cttccttcca atccagcatc
     5941 tacatttctc cggcttgagg cttttgtgtg tcgctttgtt gctggttctg gcaagcccgg
     6001 cgcgagtggc aacggcgagc ggcgagcggc gcccatcctc gtctacctcg gcgcgtccgg
     6061 ctggaactga cgttgttgcc gaccctcctg gcctgctgta tcagggagtt ccggcagaga
     6121 ttccgcagga ccagggagcg gctgggttgc acctcgaatt gctgcgcctg ggaacgacag
     6181 cgcgcctgat gcaagtggtg gcccatcccg atgatgagga cggcggcatg ctcacgctgg
     6241 aagcgcgcgg acgcggcgtc agcacgttgc tgatgacgat gaaccggggc gagggcgggc
     6301 aaaacaaggt cggcagcaat ctgtcggatg tgttgggcgt gctgcgcgcg ctcgaactgc
     6361 tcgcttctga tcagtactac ggtatccagg aacgtttttc gcacgtcgcc gactttggat
     6421 tctccaagag cgcggacgag accttcgcca agtggggcgg acatgacccg gcgctcggcg
     6481 acatggtgcg cgtgatacgc acctttcgtc ccgatgtgct ggtggcgcgt ttttcgggca
     6541 ccgagcgcga cgggcacgga aatcatcagg cttcgagcat cctggcgaag gaggcattcc
     6601 gcgcggcggc cgatcccagg cgcttccccg agcagatcgt cgaggggctc gaaccctggc
     6661 aggtgaaaaa gttctacatc ggcaacgtct gcggattcgg tgctatgact tgccccgacg
     6721 ctgactggac gctcaagctg aattccggcg agcgcagcac ggatcttggc atgtcgtacg
     6781 tgcagttcgc catggaagga ctgcggcatc agttgtcgca aggggcggcc aattggaccg
     6841 tcgacccggg cgatcgtttc a
//
LOCUS       DASPUL010000205        16579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1539555, whole genome shotgun sequence.
ACCESSION   DASPUL010000205 DASPUL010000000
VERSION     DASPUL010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 16579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16579)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16579
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539555"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            556..1494
                     /locus_tag="VEJ00_04810"
     CDS             556..1494
                     /locus_tag="VEJ00_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXX00512.1"
                     /translation="MERPAAAESSKSAPIVASLQGVKKSYGDVRALHDVDFEVRAGEV
                     VALLGPNGAGKTTAVKLLLGLMPPNSGQVLVFGCDPTNPENRMRTGAMLQVGRVPETL
                     RVREHIDLFSSYYRKPMAPAAVLAAAGLDQLGDRKFGDLSGGQRQRVLFALAICGDPD
                     LLFLDEPTVGLDVEARRIVWDEIRQLVRRGKTVLLTTHYLQEADALADRIAVINKGKI
                     IAEGTPTEIKAKTSGKRVRCLTSLGIDSVKQIPGVLGVRLDREAIEIDAVVAEPVVRE
                     LMMRDAQLSGLEISSAGLEEAFLALTQENTSGNERH"
     gene            1539..2357
                     /locus_tag="VEJ00_04815"
     CDS             1539..2357
                     /locus_tag="VEJ00_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX00513.1"
                     /translation="MSVATAVLTQPRIERSRSQTAEIYFKEAKYEFLKNLRLRVYSLS
                     VISFPLMFYVLFGLVLNPKNAPVGGTTITSYLMATYGTFGVMGASLFGTASALAAERG
                     LGWLQLKRATPMPPFAYFSAKVVMSMIFSATVVLLLMLLGVLFGGVRMAVLDGAKLLG
                     TLVAGCLPFAAMGLAIGYFAGPNSAPSTINLIYLPLSFCSGLWVPYMFLPRFVRQVAQ
                     VLPPYHLSQLALGVFHAGQHEPASRHWEVLLAFTLICLGIARLGFQREQEKMYG"
     gene            2420..2671
                     /locus_tag="VEJ00_04820"
     CDS             2420..2671
                     /locus_tag="VEJ00_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00514.1"
                     /translation="MACSAILVMLGVFAIYGGVRLLALLLPAAVLVWYEWSVSQRRGK
                     DRQLGPTAGMAGWIDHEWDNRTMRTEAGPKRMRTARRAE"
     gene            2668..3768
                     /locus_tag="VEJ00_04825"
     CDS             2668..3768
                     /locus_tag="VEJ00_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXX00515.1"
                     /translation="MRLLPKNSEYTWAPYSALYFGFFLLDPIESHAGARVWLFTMLGT
                     IAFLFLYFGMFWVSRRRAMLHVAAMVLLGAAYARYNGGATTFFIFAAASAPFILQTEA
                     DGFKALAGIEAAVLLTWWAFHLSGWFLLFGGGLSLVIGLSNIHFAQRNRTQKQLIHAQ
                     EEIEHLAKVAERERIARDLHDVLGHTLSVITLKSELARKLIDRDPQRAGKEIGEVEEI
                     SRQALAEVRDAIRGYRAQGLAAELTRAKSTLETAGLSVQCDAASSLQLPAMQESVLAL
                     AVREAVTNVVRHARASSCQLRLEQQNGSCRLEIRDDGVGCASLEGNGLRGMRERVEML
                     GGTLDRTTQSGTALTITLPLTEPVSKEEGGQK"
     gene            3765..4406
                     /locus_tag="VEJ00_04830"
     CDS             3765..4406
                     /locus_tag="VEJ00_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXX00516.1"
                     /translation="MNPPARDAKEGRKTIRVVLAEDQGMVLGALAALLEIEGDISVVA
                     QARNGKEALEAVLTHKPDVFITDIEMPHMTGLDVAAELKRRRTGTRVVIVTTFARAGY
                     LRRALEAGASGYLLKDMRAEELADAVRRVHQGLRVIHPELATEAWGELDPLTDRERQI
                     LRLAGEGMASGDIAAELRLSDGTVRNYLSEAISKLGASNRVEAARIARTKGWL"
     gene            complement(4419..4766)
                     /locus_tag="VEJ00_04835"
     CDS             complement(4419..4766)
                     /locus_tag="VEJ00_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00517.1"
                     /translation="MEGPSIRECLTAAIRYWEPRRIVYNLVLAAVVAVYYLICLPSSR
                     HALTTDSILVLFLLAVLANVAYCAAYVVDIFAQMSNFRDLWLSGRWIVFVVGMAFAAV
                     LTRVFSLGMFGVP"
     gene            complement(4808..5761)
                     /locus_tag="VEJ00_04840"
     CDS             complement(4808..5761)
                     /locus_tag="VEJ00_04840"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HXX00518.1"
                     /translation="MPIDPVLVVHGGAWAMPDDMVEAHIRGVKNALAAGWRVLDRGGS
                     ALDAVEEAVVIMEDDETFDAGRGSFLNRDGKVQLDALIMDGATLRAGGVGCVEHLRNP
                     VRAARKILSESPHVYFVGEGAEKFAAQHGIPLCRNEDLIIPREVERLRQYQAEAARRD
                     AKPDGNHLFAPSADDATISHDTVGAVALDRNSNIAAATSTGGTLNKAPGRLGDSSLIG
                     CGCYADNESAAASTTGWGEPIMKLVLAKWTADRIAAGNLPEWAAQEAMNYLKQRVNGH
                     GGIIVLNPEGHIGIAHNTPRMAWAYKTVKKQETGIERDGSK"
     gene            complement(5846..7954)
                     /locus_tag="VEJ00_04845"
     CDS             complement(5846..7954)
                     /locus_tag="VEJ00_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HXX00519.1"
                     /translation="MSSRRKFLSEVALAGAAFASADPELLSSETPAACAVAALVSLRG
                     KWRFQTDPENAGTKAGWHATDFSAPNWHEVVVPHTWQVDPAYSDFRGVAWYRRTFDAL
                     QDWRDAAVRLEFEAVFHTATVWVNGQLVGEHSRKGYTEFSFDITSALRWDQPNVLAVR
                     VDGAFNEHMLPRGRSSDWAHDGGIFRPVHLVVTPKTFIENIHVEAVPNLNTGIATITI
                     LAHCRNTGAAAFGGTAAYRIIDDDTGLTAQAGTHATKLIVKPAASELLTLRIELPGAK
                     LWHFDSPHLYRLECRITDGRQDHQFETTFGVRSFEVQDGKFHLNGEPVRLMGVERLAG
                     SNPQFGMAETDAWIARDHDDLKKLNCVFTRVHWPQDKRVLDYCDRHGILMQLEVPAWG
                     PDTFKGMTAQPDSDIMENGLEQLREMIQSDGNHPSVVAWGLCNEIDGQNPPAHEFAKR
                     LLEEAKRLDPRRLCSYASHSLRETPEKDVAGLMDFIEANEYFSTWHSGNIHDLDKHLD
                     SLHAVFPGKPIVISEYGYCACTPDRPEGDSHRIDILRTHDEVFRSKAFVSGAIFFCYN
                     DYRTHVGDRGTGALQQRVHGVVDLYGARKPSYDVLRHELSPIESLTIENRLNSFHLLL
                     KSRTTVPAYTLRGYKLRAVFYGQGDIPVEVQEASLPELPPGQTAETNLTFAQSEVPRC
                     VRFEVIRPTSFPAFSMEWNP"
     gene            complement(8030..8671)
                     /locus_tag="VEJ00_04850"
     CDS             complement(8030..8671)
                     /locus_tag="VEJ00_04850"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXX00520.1"
                     /translation="MTVSWTSAFGRSCGVLLDTIVRWLALSRINPNVLTFMGLVVNTW
                     AAILFGSANADNQKRMFIYAGLVIIFSGFFDLVDGQVARATNRVTRFGAFFDSVVDRY
                     SDASQFLGLLVFYARGERFFYVVLSAFVMISAIMVSYTRARAESLIGSCRVGFMERPE
                     RLVLVILGALFNRMAPVLWVIAVLSTITVIHRMRYTWTRTQDPPVALTPGQAA"
     gene            complement(8787..9287)
                     /gene="greA"
                     /locus_tag="VEJ00_04855"
     CDS             complement(8787..9287)
                     /gene="greA"
                     /locus_tag="VEJ00_04855"
                     /inference="COORDINATES: protein motif:HMM:NF001263.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HXX00521.1"
                     /translation="MTDQIRRKLQEEINALEYELVHELPKEIKKAAALGDLSENAEYH
                     MAKQRQEYVKARVRQLGKRLADLSLINMNNIPRDKVGLGSTVKVFDNTKNEEVEYKLV
                     TSEESDVAAGKISTTSPIGRALLHKKVGDTAMVVTPNGKRELEILSLSTIHDEAEDDE
                     PQEAGA"
     gene            complement(9387..10553)
                     /locus_tag="VEJ00_04860"
     CDS             complement(9387..10553)
                     /locus_tag="VEJ00_04860"
                     /inference="COORDINATES: protein motif:HMM:NF025433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HXX00522.1"
                     /translation="MSRFSRQYPLILLLVLAGTIAARAQQTSDSAPAASPAQTAAVQT
                     SYTVQVDGTKEWTDTKIDVRGGEKLKISADGTITYTDKNKSFGPTGIPRGFADLIHQY
                     AVPNGGHGELIGRLGSGNTGEAFEIGNGITFTAPVAERLFLGINQSLKDAATATGNFQ
                     VKIEVLDQGAVTTVGGPAESTIPGITLDLLESLPRRVADEQHNPGDMVNVLLIGSEDD
                     VVKTFTTGGWVKVDKSVGSTVMAGLLDTMEKKDYLTMPMSTLYLFDRPQDYGFAHAEP
                     VRVVMSRNHLRVWKSPYMVNGRQLWCVAATHDIGFERDQRNNGVTHKIDPAIDGEREY
                     VNDTLSSTGLVVARGHVTPKNALTTAKTATGGEFHSDGRILVLSLKSADSEAKN"
     gene            complement(10658..11194)
                     /locus_tag="VEJ00_04865"
     CDS             complement(10658..11194)
                     /locus_tag="VEJ00_04865"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HXX00523.1"
                     /translation="MSGPDLNFHVGDKVVYPNHGVGVIEQIGSRTLGANVERFYLLHI
                     KASSLKVMVPCHNAASVGLRRVVRNGDVQKVLDLLSVSDNGSNGDWKDRFKENSDRMR
                     TGSLLEVAGVLKSLIALHQAKPLSFREKKMLERARYLLVSELAMARNCEEAKVEELLT
                     ATLAKCNLRFPEASEFQA"
     gene            11338..11871
                     /gene="ruvX"
                     /locus_tag="VEJ00_04870"
     CDS             11338..11871
                     /gene="ruvX"
                     /locus_tag="VEJ00_04870"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HXX00524.1"
                     /translation="MSKSSDANSREPAMPHAEDVQARSPSQGRVLGLDVGSRRIGIAV
                     SDPLGITAQGLETLQRTNKRQDFEYLRRVIDQYEVREIVVGLPLRMSGAEGTQSEKMR
                     AFAEELAKTFKLPVHLWDERLTSAEANRFLRETELSIEKRGKAVDRMAAVLILQAWME
                     QRAASASGSRLPASGPD"
     gene            11946..12650
                     /locus_tag="VEJ00_04875"
     CDS             11946..12650
                     /locus_tag="VEJ00_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HXX00525.1"
                     /translation="MRSVIALIVLSLIALIAQGQVSCPNPKIVKTTGTAELKVTPDKA
                     LIQLGVERQSATAKNAKAAVATASRKILAELKGLGIEDKDIQTAYLYLQPMIDYRKGL
                     RITNFTAEQSLSVTLRDLSKLDSVMDAVISAGANHIEGIEYQSSDLRKYKDQARDEAA
                     KAAREKAEALAKALGNQIGKTYSIEEEPQREGYYGVGGGYAPNIALDKMESRAASTAP
                     GELTVKASVVVSFDLL"
     gene            complement(12706..13731)
                     /gene="mltG"
                     /locus_tag="VEJ00_04880"
     CDS             complement(12706..13731)
                     /gene="mltG"
                     /locus_tag="VEJ00_04880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXX00526.1"
                     /translation="MKKLVWLILVAVIGAGAWFAWAVMTPVEPAGQTFVLLRPGYSTR
                     RIAAELKNAGVIRSEEAFVLWHYVHRKRSLKAGEYLFDKSADIIDIQKRLRRGDVYFH
                     TVVVPEGYTMFDISRAIESAGLGPAEDFVKAAQSDSALVAGIDPGAPSLEGYLFPDTY
                     EFTRLMTMHDMAAAMVKQFRQVYTQIQQSGDPSADIAYEQAWRTRSPHEIVTIASIIE
                     KETAVPEERPMVASVYYNRLAKHMALDADPSIIYAELLAGTYQGALHHADMQFSSPYN
                     TYRNAGLPPGPIGNPGRSALEAAMHPAQSDFYYFVADAQGHHRFARTMEEHNKNVIAY
                     RRAVRGR"
     gene            13980..14906
                     /locus_tag="VEJ00_04885"
     CDS             13980..14906
                     /locus_tag="VEJ00_04885"
                     /inference="COORDINATES: protein motif:HMM:NF025490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HXX00527.1"
                     /translation="MRRSSPFWASIVLLGMLPTTGCLFRTRPVEDRYSKVPLKESSQQ
                     GLIDSINQQAETIHSLKATVDIDSSAGGVKKGHVTDYKEIRGYVLARKPASLHMIGLM
                     PIVRTTAFDMVSDGQDFKLWIPPKNRFVLGKNDVPTANTDQPMENIRPQNFYEALLIP
                     RIDPESEIAVLENGYETLHDGKGHRILQEDYELTVIQKRDKGWALERKIIFGRTDLKP
                     HRQFIYDQDGKVATDARYAEYKEYNGVSFPSRIEISRPQEEYDITLNMLKVDINKPLT
                     DDQFVLEQPAGAEVVHLDHPQSSLVRPVGNRE"
     gene            15092..16186
                     /locus_tag="VEJ00_04890"
     CDS             15092..16186
                     /locus_tag="VEJ00_04890"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX00528.1"
                     /translation="MVVANLVHRPIRSLISVIAIALEVTLILLIVGLCYGIMNDSKSR
                     TSGIGFDVIVQPPGSSFFSAISGAPVSVKVADVLRKMPHVKVVSPVVWQIATGGGVEV
                     IDGIDINSFEALGGPFRYVHGGPFHGPDDVLVDDFIARQRRVKVGDTMMILDHPFHVC
                     GVVENGRGARKFVPMGTMQDLIGANGKASVFYMKLDDPANTDQVVEAVKKQPGMENYS
                     VRSMQEYLSMMTPSNLPGFRPFIGVVIGVSLVIGFLVIFQSMYTAVMERTREIGILKS
                     MGASKYYIVSVVLRETVLLAITGILVGIAVSLMARIGIQHRWPLVRIDKSTEWIIRAT
                     IIAVLGATGGALYPAYKAAQKDPIDALAYE"
     gene            complement(16221..>16579)
                     /locus_tag="VEJ00_04895"
     CDS             complement(16221..>16579)
                     /locus_tag="VEJ00_04895"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HXX00529.1"
                     /translation="ARNRSVSDDREALYDKLIATNPEIERKGDANPYTAVNGNMFTLL
                     HESRLAIRLPEEEREKFLKKYKTKLYEAYGTVMKEYVAVPDEMLANTKELKKYLDLSY
                     EYARTLKPKPTTKKKR"
BASE COUNT         3318 a         4828 c         5023 g         3410 t
ORIGIN      
        1 ctggaattct ccacatcgcg catagtaagg ccaagcttct cgcgcagtgt gcgaagactt
       61 ttgccagcgg gcagcatcct gagccctcca agtggctgca tgataccaaa gccggaacac
      121 atggtgcgga ggaaatgtgg aaaactgtcg gaagaacgag cttgttacga attccggaca
      181 cgcgctgtgc cttgtgcagc gcccgtcccg gaagcggtcg ctcggcgatc taagcagatc
      241 ggcgtctact catgtctgtg gatttcgctg tgcgtgtcct gaattcgtaa cacaaccccc
      301 gaactgagct gggagagcaa ttaaggatgt cccatcctgc cccggagtgc cagctctgtc
      361 tcctttcttc ctaagctgac gatctcacac acgaaacccg agcatgacat ttgtcaccct
      421 cgctgagtga tggatggcac tggcacaagc ctctgccgcg agccatgatg cgagcgttgg
      481 atgatgaacc ggcggggcgc tcgcgcggcg cccaggcccg ttgaatacgc ttggaggacg
      541 cagatgattg ccaccatgga acgaccagcc gcggcagaaa gcagcaagtc agcgccgatc
      601 gtggccagcc ttcagggtgt gaagaagagc tacggtgatg ttcgtgccct gcacgatgtg
      661 gattttgagg tgcgtgcggg cgaagtggtc gctttgctgg gaccaaacgg cgcgggcaag
      721 accaccgcgg tcaagcttct gttgggtctg atgccgccta attccgggca agtgctggtt
      781 ttcggctgtg atcctacgaa tccggaaaac cggatgagaa cgggcgccat gttgcaggta
      841 ggacgagttc cggagacttt gcgcgtccgc gaacacatcg atttgttctc ctcctactat
      901 cggaaaccga tggcgccggc ggcggttctc gcggctgccg ggcttgacca gctgggcgat
      961 cgtaagttcg gcgacctctc gggaggccag cggcagcgag ttctttttgc gcttgctatc
     1021 tgcggcgatc ccgacctgct ctttctcgac gaacccacgg tcggcctcga cgttgaagct
     1081 cggcgaatcg tctgggacga gattcgccag ttggtccgcc gcggcaagac cgtgctgctc
     1141 accacgcact atctgcagga agccgatgcg ctggccgacc gcatcgcggt gatcaataag
     1201 gggaaaatca tcgcggaggg cacgccgaca gagatcaaag ccaaaaccag cggcaagcgg
     1261 gttcgctgcc tgaccagtct aggaatcgac agcgtaaagc aaattccggg cgtgctcgga
     1321 gtcaggctgg atcgcgaggc gatcgagatt gacgcagtcg tcgccgaacc agtcgtacgg
     1381 gaattgatga tgcgcgatgc gcagctttcg ggtctggaaa tttccagtgc cggcctggag
     1441 gaagccttcc tcgcgctgac ccaggagaac accagcggaa atgagcggca ttgaagtcat
     1501 ccggatccca aaaagagatc accaaggaga acaaggatat gagcgtagca acagcggtgt
     1561 tgactcagcc ccggatcgaa cgttcccgca gtcaaacggc tgaaatctat ttcaaggaag
     1621 caaaatacga gttcctcaag aatcttcgcc tgcgcgtcta ctcgctgtcg gtgatcagtt
     1681 tcccccttat gttctacgtg ttgttcgggc tggttttgaa tccgaagaac gcgccggtgg
     1741 gaggaacaac gatcacctct tatctcatgg cgacctacgg cacatttgga gtgatgggag
     1801 catcgctgtt cggtacggct tcggcgctgg ctgccgagcg ggggctgggc tggctccagc
     1861 tgaagcgcgc cactcccatg ccgccctttg cttatttctc ggcgaaggtg gtgatgagca
     1921 tgatcttcag cgcgacggtg gtgttgctgc tgatgctgct cggcgttctg ttcggaggcg
     1981 tccggatggc ggtgctcgat ggcgcaaaac tgctgggcac gctcgtggcc ggctgtctcc
     2041 cgtttgccgc tatggggcta gcgatcgggt actttgccgg gcccaactcc gcgccttcga
     2101 ccatcaacct catctacctt ccgctttcat tttgcagcgg gctttgggtg ccctacatgt
     2161 tcctgcccag gttcgtgcgc caggtggcgc aggttttgcc gccttatcat ttgtcgcagc
     2221 tcgcgctagg cgtgttccac gctggccagc acgaacctgc ctcccggcat tgggaagtcc
     2281 tgctggcctt cacgctgatt tgcctgggca tcgcccgcct cggattccag cgtgagcagg
     2341 agaagatgta tggctaggag caggcagaca atgctgttga atcgggagga tcagttcatg
     2401 aaccgaactc aagaactcga tggcatgcag cgcgatcctt gtgatgctag gcgtgttcgc
     2461 gatctatggc ggcgtccggc tgctggcgct actgcttccc gccgcagtgc tggtctggta
     2521 tgagtggtca gtttcgcagc gccgcgggaa ggatcgtcaa ctgggtccaa ccgccggtat
     2581 ggccggctgg attgaccacg aatgggacaa tagaaccatg cgtaccgaag cagggccgaa
     2641 acgaatgcgg acggcacgga gggccgagtg aggctcctcc ccaagaattc cgagtacacc
     2701 tgggcgccgt actccgccct ttatttcggt tttttccttc tggatcccat tgagagccac
     2761 gccggcgcgc gtgtgtggct ctttaccatg ctggggacga tcgcctttct ctttctctat
     2821 tttggaatgt tctgggtcag ccggcggcgt gcgatgctgc acgtggctgc catggtgctg
     2881 ctgggcgcgg cctacgcccg ttacaacggg ggcgcaacca catttttcat cttcgccgca
     2941 gcctcggcgc ctttcatcct ccagaccgag gcagacggtt tcaaagccct cgccgggata
     3001 gaagcggcgg tgcttcttac ctggtgggcc tttcatttga gcggatggtt cctgctgttt
     3061 ggcggcggcc tctcgctggt catcggcctc agcaacattc actttgccca gcgcaatcgc
     3121 acccagaagc agctgattca cgcccaggaa gaaatcgagc atctggcgaa agttgccgag
     3181 cgcgagcgga ttgcgcgcga tttgcatgac gtgctggggc acacattatc ggtgattacg
     3241 ctgaaatcgg agctggcgcg caagctcatc gatcgcgatc cgcaacgcgc gggaaaagag
     3301 atcggcgaag tcgaggagat ctcgcggcag gccctcgccg aggtgcgcga tgccattcgc
     3361 gggtatcgtg cgcagggtct ggctgccgaa ctgacgcgcg ccaagtccac gctcgaaacg
     3421 gctggcctgt ctgtgcaatg cgatgccgca agcagcctgc aactgcccgc aatgcaggaa
     3481 agcgtccttg cactggccgt gcgggaagct gtcacgaatg ttgtgcgaca cgcccgcgcc
     3541 agcagttgcc agttgcgcct ggagcagcag aacggctcct gccgtctcga gattcgcgac
     3601 gacggcgtcg gctgcgccag cctcgaaggc aacggactgc gcgggatgcg cgagcgcgtc
     3661 gaaatgctgg gcggaacgct cgaccgcacc actcaatctg gaacggctct gaccatcacg
     3721 ctgccgttaa cggaacccgt gtcgaaagaa gaaggtgggc agaagtgaat cctcctgcaa
     3781 gagacgcaaa ggaaggccgc aaaaccatcc gcgtggtgct ggctgaagat cagggcatgg
     3841 tgctgggcgc actcgctgcc ctgctcgaaa tcgagggcga catctccgtc gtcgcgcagg
     3901 cgcgcaatgg aaaagaggcg ctagaagcag tcctcacaca taaacccgat gttttcatta
     3961 ccgatattga gatgccgcac atgaccggcc ttgacgtagc ggcggaacta aagcgcagac
     4021 gcaccggaac gcgcgtggtc atcgttacaa cgtttgcccg cgccggatac ttgcgccgcg
     4081 ctctcgaagc cggagcttcg ggatatttac tgaaagacat gcgcgccgaa gaattggcgg
     4141 acgccgtccg ccgcgtacat caaggactgc gtgtgatcca tcccgaactc gccaccgaag
     4201 cctgggggga actcgatcct ctcacggacc gcgagcggca gatcctgcgc ctggccggag
     4261 aaggcatggc cagcggcgac atcgcggccg aactgagatt gtctgacggc accgtgcgca
     4321 actatctttc cgaagccatc agcaagctag gagcgagcaa tcgcgtggag gccgcgcgga
     4381 tcgcgcgaac taagggttgg ttgtaaaacc gggaatttct agggcactcc gaacattccc
     4441 agcgaaaaga cgcgcgtcaa taccgcggca aaggccatac ccacaacgaa gacgatccat
     4501 cggcccgaga gccagagatc ccggaaattc gacatctggg caaagatgtc gacgacatac
     4561 gccgcacagt aggcgacgtt cgccagcacg gcaagcagaa acaaaaccaa aatcgagtca
     4621 gtagttagcg cgtgtcgcga cgagggcagg caaatcaagt agtaaaccgc gaccactgcc
     4681 gccagcacga ggttgtacac gatgcgcctt ggctcccaat aacggatcgc cgcggtaaga
     4741 cattcgcgaa tggagggacc ttccatcacc gcttgtaatg cggaaccgat tctactgctg
     4801 taccgaccta ctttgaccca tcccgctcta taccagtttc ctgctttttc accgttttgt
     4861 atgcccaggc catgcgcggg gtgttgtggg cgatgccgat gtggccttcg ggattgagca
     4921 cgatgatgcc accgtgtccg ttgacgcgct gcttgagata gttcatcgcc tcctgcgcgg
     4981 cccattccgg cagattgccg gcagcgatac gatcggcggt ccacttcgcg agcaccagct
     5041 tcatgatcgg ctcgccccag cccgtggttg aagcggcagc gctctcgttg tcggcgtagc
     5101 agccgcaacc gatcagcgac gaatcgccga gacgtcccgg agccttgttc agcgtacccc
     5161 ccgtggaagt cgccgcggcg atgttgctgt tgcggtcgag tgcaaccgcg ccaaccgtgt
     5221 cgtgcgagat ggtcgcatca tcagccgacg gggcgaagag gtggttgcca tcgggctttg
     5281 catcacgcct ggcagcctct gcctgatact ggcgcaatcg ctcgacctca cgcggaataa
     5341 tcaggtcttc gttcctgcac agcgggatgc cgtgctgcgc ggcaaacttc tccgcgcctt
     5401 cgccgacaaa ataaacgtgc gggctttcac tcagaatctt gcgcgcggcg cgtaccggat
     5461 tgcgcaagtg ttcaacgcaa cccacgcccc cggcgcgcag cgtggcgccg tccatgatca
     5521 aggcgtcaag ctggactttt ccgtcgcgat tcaaaaagct gccgcgcccg gcgtcgaaag
     5581 tctcgtcgtc ctccatgatg acgaccgctt cctcgaccgc atcgagcgcc gagccgccgc
     5641 ggtcgagcac gcgccatccc gcggccagcg cgttcttcac gccgcggata tgcgcctcga
     5701 ccatgtcatc gggcatggcc caggcgccgc catgaacaac cagcacggga tcgatgggca
     5761 agagttgtcc ccagaagtgg tggtgcgact cgatagtcta gcaggtgcaa ggatccctgc
     5821 ggtgtggagg cccgcgaggg aaaccttacg ggttccactc cattgaaaac gccgggaacg
     5881 aggtggggcg gatcacttcg aagcggacgc agcgcggcac ttcgctctga gcaaacgtca
     5941 aattggtttc cgccgtctgt cccggcggca gctcgggcag ggacgcttct tgcacctcca
     6001 ccggaatatc cccttgccca taaaacactg cacgcagttt atagccgcga agcgtgtagg
     6061 cgggcacggt ggtgcgtgac ttcagcaaca gatgaaagga gttcagccgg ttctcgatgg
     6121 tgagcgattc aatcgggctt aactcgtgtc gcaagacgtc gtaggagggc tttctcgcgc
     6181 catacaggtc aaccactcca tgcacccgtt gctgcaatgc tcctgtacct cggtcaccga
     6241 catgggtgcg gtagtcgttg tagcagaaaa aaatcgctcc acttacaaag gctttcgatc
     6301 gaaagacttc atcatgggtg cgcaggatat cgatgcgatg gctgtcgcct tccggccggt
     6361 ctggcgtgca ggcacaatag ccgtactcgg agatcacgat tggcttgccg ggaaataccg
     6421 cgtggaggga gtcgagatgc ttatcgagat catgtatgtt tccgctgtgc caggtactga
     6481 agtattcgtt agcctcgatg aaatccatca atccggcaac atccttttcg ggagtttctc
     6541 tcagcgagtg tgaggcgtac gaacacaaac ggcgtggatc gagccgtttg gcttcttcca
     6601 ggagccgttt ggcaaattca tgcgccggag gattctggcc atcgatttcg ttgcaaagcc
     6661 cccaggccac aacggaggga tggttgccgt cgctctgaat catttcccgc aattgttcca
     6721 ggccattttc catgatgtcg gaatcgggct gtgcagtcat gcctttgaaa gtgtcgggcc
     6781 cccaggcggg aacctccaac tgcatcagga tgccgtgacg gtcgcagtag tccagaactc
     6841 gcttgtcctg cggccaatgg acgcgggtga aaacacaatt cagctttttg aggtcgtcat
     6901 gatcgcgcgc gatccaggcg tcggtttcgg ccatgccgaa ctgcggattg ctcccggcca
     6961 ggcgttcgac gcccatcaac cgcaccggct cgccgttcag atggaatttg ccatcctgga
     7021 cttcgaagct tcgcacgcca aaggtcgttt cgaactggtg gtcctggcgg ccgtcggtaa
     7081 tccggcattc cagccggtaa aggtgtggag aatcgaagtg ccacagcttg gcgccgggca
     7141 gttcaattct taatgtgagc agttcacttg ctgccggctt gacgatgagt ttcgtggcgt
     7201 gggtcccggc ttgtgccgtg agtcctgtgt cgtcatcaat gatgcggtaa gcggctgttc
     7261 cgccgaaggc cgctgcgccg gtatttcggc agtgagccaa gatagtgatc gtggcgatgc
     7321 ccgtgttgag attgggaacg gcctcgacgt gaatgttctc tatgaacgtc tttggcgtaa
     7381 cgacgaggtg aaccgggcgg aagatgccgc catcgtgcgc ccagtcgctg gaacgccccc
     7441 ggggcagcat gtgctcgttg aacgcgccat ccactcgtac ggcgagcaca ttgggctggt
     7501 cccagcgaag cgccgatgtg atgtcgaacg agaactccgt ataccctttg cgtgaatgct
     7561 ctccgaccaa ctggccgttg acccagaccg tggctgtatg aaacacggcc tcaaactcca
     7621 ggcggacagc ggcatcgcgc cagtcctgaa gcgcgtcgaa agtgcgccga taccacgcga
     7681 ctccccgaaa atcagaatag gcaggatcga cctgccaggt gtggggcacg accacttcat
     7741 gccagttggg tgctgagaag tccgtggcgt gccagcccgc tttcgtgcct gcgttttcgg
     7801 gatcagtctg aaatctccat tttccgcgca ggctgaccaa ggcagcgaca gcacatgctg
     7861 ctggcgtttc agaactcagc aactcgggat cggctgaagc gaaggcggcc ccagcgagag
     7921 ccacctcgct cagaaatttg cggcgggaag acatggctat gccggcagga aagtgccagt
     7981 taaccagatc gtcaggcgca tgtctacact cgcaccggaa aagtttggtc taggcggctt
     8041 ggcctggcgt cagggccacg ggcgggtctt gggtacgggt ccaggtgtag cgcatgcggt
     8101 gaatcaccgt aatcgtactt agcaccgcaa tcacccacag cactggcgcc atgcggttga
     8161 acaacgctcc taggatgacg aggacgaggc gttccggccg ctccatgaat ccgacgcggc
     8221 aggaaccgat gagggattcg gcgcgggcgc gggtgtagct gaccatgatg gcgctgatca
     8281 tcacgaatgc cgacagcacg acgtagaaga agcgctcgcc gcgggcgtaa aagaccagca
     8341 atcccaggaa ctgcgacgcg tcgctatagc ggtcgacgac cgagtcgaag aacgccccga
     8401 agcgggtgac gcggttggtg gcgcgggcga cctgcccgtc gaccagatcg aagaagccgg
     8461 agaagatgat caccagtccg gcatagatga acatgcgttt ctgattgtcg gcattggccg
     8521 agccgaaaag gatggcggcc caggtgttga ccaccagccc catgaaggtc agcacattgg
     8581 gattgatgcg cgacagcgcc agccagcgca cgattgtgtc gagaaggact ccgcaggaac
     8641 ggccaaaggc ggaggtccag gagaccgtca ttgcagacgc tcccgcttca cctcacgctt
     8701 aagtgtagta gcgatgcgcg cgagatccct cgccccgctg gtgaaaacgc ggggcttcgg
     8761 gatgacgcca tgtatcgaga aggccattac gcccccgcct cttgcggttc gtcgtcctcg
     8821 gcttcgtcgt gaattgtgct caggctgagg atttccagtt cgcgcttgcc attgggcgtg
     8881 acgaccattg cggtgtcgcc cactttctta tgcagcaggg cgcgtccgat cggcgaggta
     8941 gttgaaatct tgccggcggc gacgtcagac tcttcgctcg tgaccagttt gtattcgact
     9001 tcctcgtttt tcgtgttgtc gaagaccttg accgtcgaac ccagccccac cttgtcgcgg
     9061 gggatattgt tcatgttgat cagcgagagg tcggccaggc gcttgccgag ctggcgcacg
     9121 cgcgccttga cgtactcctg gcgctgcttg gccatgtgat actcggcatt ctcgctcagg
     9181 tcgcccagag cggcggcttt tttgatctct ttgggcagtt cgtggaccaa ctcgtattcg
     9241 agggcgttga tctcctcctg cagttttcgt ctgatttgat cggtcatgga tgactacacg
     9301 ccagatatgg caaggcttgg gacattataa acccgcctga gatgccacag gccgagagaa
     9361 tgccccgcag ttccattctg gttcgttcag ttcttggctt ccgagtctgc gctcttcagc
     9421 gacagcacca gaatccttcc gtcggagtga aactcgccgc ccgtagccgt cttggcggtg
     9481 gtcagagcgt tcttgggtgt cacatgtccg cgcgcaacga ccagtccggt cgatgagagg
     9541 gtgtcgttca cgtactcgcg ctcgccgtcg atcgccggat cgatcttgtg cgtcactcca
     9601 ttgtttctct gatcgcgctc gaacccgata tcatgcgtcg ccgcaacgca ccacaactgg
     9661 cgtccgttca ccatgtaggg cgacttccac acccgcaggt gattgcgcga catcacgacg
     9721 cgcaccggct cggcgtgggc gaaaccgtag tcctgtgggc ggtcgaacag gtacagcgtg
     9781 ctcatgggca tggtcaggta gtctttcttt tccatcgtgt ccagaagccc tgccatcact
     9841 gtgcttccta cagacttgtc gaccttgacc catccgccgg tggtgaaggt tttcacgacg
     9901 tcatcctcgc tgccgatcag caggacgttc accatatcgc cgggattgtg ctgctcgtcc
     9961 gcgaccctcc gtggcagcga ctccagcaag tccagagtaa tgcccggaat cgtgctctct
    10021 gccggacctc cgaccgtcgt cactgccccc tggtccagca cttcaatttt cacctggaag
    10081 ttgcccgtcg ccgttgcggc atccttcaga ctctggttga ttccaaggaa taaccgctcc
    10141 gccacgggag cggtgaaggt gatgccgtta ccgatctcaa aagcttcgcc ggtattgccc
    10201 gagcccagcc gtccaatcag ttcgccgtga cctccattcg ggactgcata ttggtgaatc
    10261 aggtcggcaa agccgcgcgg gatgccggtg gggccgaaac tcttgttctt gtcggtgtag
    10321 gtaatggttc cgtcggcgga gatcttcaac ttctcgccac cgcggacgtc gatctttgtg
    10381 tccgtccatt ccttcgttcc gtccacctgc acggtgtacg aggtttgcac ggcagccgtt
    10441 tgggccgggc tggcggcagg tgccgagtcg gatgtttgct gtgccctcgc cgcgattgtt
    10501 cccgcaagca cgagtaagag gatcagagga tactgacgac tgaaacgtga catatgcaat
    10561 ttcctcctct caaccgcgga tgctaccaca ggctccgctg ggtgttaaac gggcgaaggg
    10621 agtcaatggg ctgcggccaa gacatatcag gaaggtgcta tgcctggaat tctgaggctt
    10681 ccgggaagcg caggttgcac ttggccagcg tcgccgtgag gagttcctcc accttggcct
    10741 cctcgcagtt gcgggccata gccaactcgc tgaccagcag gtagcgtgcc cgctccaaca
    10801 ttttcttttc gcggaaggac agcggcttgg cctgatgcaa ggcgatcaga ctcttgagca
    10861 ctccggccac ttctagcaga gagccggtcc gcatccggtc ggagttctcc ttgaaacggt
    10921 ctttccagtc gccattgctc ccattgtcgc taactgaaag caggtccagc actttctgga
    10981 cgtcgccgtt ccgcacaacg cgccgcaaac cgacgctcgc ggcattgtgg cagggcacca
    11041 tgaccttcag gctgctggcc ttgatgtgca gcaggtagaa cctttccacg ttcgcgccga
    11101 gcgtgcggct gccgatctgc tcgataacac caacgccatg attgggatag acgaccttat
    11161 cgccgacatg gaaattgaga tctggaccgc tcattgtcag gcacccaggc aggtgacagg
    11221 tcagggggaa agagctacgt tactactgtt ttcatgctac tccccggact ggcggaacgc
    11281 aaggccgggg aacctcttgg taaagcccga cgcttaaccg gccctataat gaggcccgtg
    11341 tccaagagca gcgacgccaa ctcgcgggaa ccggccatgc cccacgccga agacgtccaa
    11401 gcccgcagcc catcccaggg gcgggttctg gggcttgacg tcggctcgcg gagaatcggc
    11461 atcgccgtct ctgaccctct cggaattacc gcccaaggcc tcgaaaccct tcagcgaacg
    11521 aacaagcggc aggacttcga atacctccgt cgtgtcatcg atcagtacga agttcgggag
    11581 atcgtagtcg ggcttcctct acgcatgagc ggggccgaag ggacgcaatc ggagaagatg
    11641 cgggcgttcg ccgaagagct ggcgaagaca ttcaaactcc ccgtccacct gtgggacgag
    11701 cgcctgacct cggccgaggc caaccggttc ctccgggaga cggaattgtc gatcgaaaag
    11761 cggggaaagg ccgtggaccg aatggccgcc gtgttgattc tgcaggcctg gatggaacaa
    11821 cgcgcggcat cggcctccgg ctcccggctt ccggcttccg gcccggattg aaggtcatgt
    11881 ctgccctctc ggtcgcaagg acgccacaaa agaacgttct cacaacagca caaagggggc
    11941 gaaggatgag atccgtcatc gcgttgatcg ttttgtctct tatagcgctg atcgcacaag
    12001 gccaagtcag ttgcccaaat ccaaagatcg tgaagacgac cggaactgcc gagttgaagg
    12061 tcacgcctga caaggcgctt atccagctcg gcgtcgagcg gcagagcgcc acagctaaga
    12121 acgcaaaagc cgcggtggcg acggcatcga ggaagatcct ggcggagttg aaggggctgg
    12181 gcattgaaga caaggacatc cagacggcgt acttatatct gcagccgatg atcgactatc
    12241 ggaagggctt gcgcatcacc aacttcaccg cggaacaatc cttgtcggtg acgctccgcg
    12301 atctttccaa actggatagc gttatggacg ccgtgatctc agccggagcc aatcacatcg
    12361 agggaatcga gtaccagtcg agtgatctcc ggaaatacaa agaccaggcc cgcgatgaag
    12421 ccgccaaagc cgcccgcgag aaagccgagg cgcttgccaa agcgttgggg aatcaaattg
    12481 gcaagacata ttcgattgaa gaagaaccgc agcgtgaggg gtactatgga gtgggtggcg
    12541 gttatgcccc aaacatcgca ttggacaaga tggaatcgcg ggcagcgtca acggctcccg
    12601 gggagctaac cgtcaaggct tcggtcgtcg tcagctttga tcttttgtaa gaggcgcgac
    12661 aaccagattc ccagcaactc ccggactccg gcagccatca gccggctacc gccctctgac
    12721 cgccctccga tacgcgatca cattcttgtt gtgctcttcc atggtgcgag cgaagcggtg
    12781 gtggccttgt gcgtcggcga cgaaataata aaagtcgctt tgcgctggat gcatggcagc
    12841 ctcgagggcg ctgcgtccgg gattgccgat gggccccggc ggcaggccgg cgttgcggta
    12901 ggtgttgtac ggagaactga actgcatgtc cgcgtgatgc aacgcgccct ggtaagttcc
    12961 ggcaagaagt tcggcgtaaa tgatgctggg atcggcgtcg agggccatgt gcttggccag
    13021 ccgattgtag tagacgctgg cgaccatcgg ccgctcttcg ggcacggcgg tttctttttc
    13081 gatgatggag gcgatggtga cgatctcgtg tggagagcgc gtgcgccagg cctgttcata
    13141 ggcgatgtcg gcggaaggat ccccagactg ctgaatttgc gtatacacct ggcggaactg
    13201 tttcaccatg gcggcagcca tgtcgtgcat ggtcatcaag cgggtaaatt cgtaggtatc
    13261 cgggaagaga tatccctcca gagagggtgc gccagggtcg atgcccgcta caagcgcaga
    13321 atcggattgt gccgccttca cgaagtcctc agcgggccca agcccggcac tctcgatcgc
    13381 gcgggatata tcgaacatcg tgtagccttc cggcactacc actgtgtgga agtacacgtc
    13441 gccacgccgc aggcgcttct gaatatcaat aatgtcggca gatttgtcga agagatattc
    13501 gccagctttc agcgagcgtt tgcggtgcac gtaatgccat agcacgaacg cttcttcgct
    13561 gcggatcacg ccggcgttct tgagttcggc ggcgatgcgc cgggtggagt agcccgggcg
    13621 cagcaggaca aacgtctgtc cagcgggttc gacgggcgtc atgaccgccc aggcaaacca
    13681 cgcgccagct ccgatcactg caaccaaaat gagccagacg agctttttca cgtctactcc
    13741 gcgcgctgat tccgcctcgc gctagttaca gtttagatga tgcccgcgtc cgccgtgacc
    13801 tgacgttagt gcgtctgcct ttcgtaagtg gttggttttc cttgggattg acacctacaa
    13861 gccaatcggg gaatccgtga ttcggggcag gacacttgca gaactgtcct gcccgtctcc
    13921 tgcatccttt ccggcgctgt atactcaaat ccggggagat ggatagttca aagattttca
    13981 tgagacgtag ctcgcctttt tgggcctcaa ttgtgcttct gggaatgctg cccacgaccg
    14041 ggtgcctttt ccgcacccgc cctgtcgaag accggtactc gaaggtgccg ctcaaggagt
    14101 cctcgcagca ggggctgatc gacagcatca accaacaggc ggagaccatt cattcgctca
    14161 aggccacggt ggacatcgac tcctcggcag gcggcgtcaa gaagggccac gtcaccgatt
    14221 acaaagagat tcgtggctat gttctggcgc ggaaaccggc ttcgctgcac atgatcggcc
    14281 tgatgccgat cgtgcgcacc acggcattcg acatggtgag cgacggccag gatttcaagc
    14341 tgtggattcc gccgaaaaat cgcttcgtgc tgggcaagaa cgatgtgccc accgcgaaca
    14401 ccgatcagcc gatggaaaat atcaggcccc agaactttta cgaggcgctg ctgatccctc
    14461 gcattgaccc cgagtctgaa attgcggtgc ttgaaaacgg ctacgaaact ctgcacgacg
    14521 gcaagggcca tcgcatcctg caggaagact atgagctgac cgtcatccag aagcgcgaca
    14581 agggctgggc cctcgagcgc aagatcatct ttggccgcac cgacctgaag ccgcatcggc
    14641 agttcattta cgaccaggat ggcaaggtcg ccaccgatgc ccgctatgcc gaatacaagg
    14701 aatacaacgg cgtgagcttc ccttcgcgga tcgaaatctc ccgtccgcag gaggagtacg
    14761 acatcacgct caacatgctc aaggtggaca tcaacaagcc gctgacggat gatcagttcg
    14821 tgctggagca gcccgcggga gcggaggtcg tacatctcga ccatccgcag tcgagcctgg
    14881 tgaggccggt gggcaatcga gagtagcgag cgccgacatg tagcgacagc cgccctcggc
    14941 tgtcgggcgg agcgatgctc caccgtttat caggagcggg gtttttgcgc tgtaagtcca
    15001 gctgcaaaca tcgctaggag ataccaggcg aactatcgct cgcccggaca gccgagggcg
    15061 gctgtcccta cacgttctca tgatgaataa aatggtggtc gccaacctgg tgcaccggcc
    15121 gatccggtcg ctgatcagcg tgattgccat cgcgctcgag gtcacgctga tcctgctgat
    15181 tgtcgggctg tgctacggca tcatgaacga ctccaagagc cgcacttccg gaatcggctt
    15241 cgatgtgatc gtgcagccgc cggggtcgtc gttcttttcg gcgatcagcg gcgcgcccgt
    15301 ttccgtcaag gttgccgacg tgctgcgcaa gatgccccac gtcaaggtgg tgagtccggt
    15361 ggtctggcaa attgctaccg gaggcggagt cgaggtgatt gatggcatcg acataaacag
    15421 cttcgaggct ctgggcggcc ccttccgcta tgttcacggc ggaccatttc atggcccgga
    15481 tgatgttttg gtggacgatt tcattgcgcg ccagaggcgc gtgaaagtcg gcgataccat
    15541 gatgattctc gatcacccgt tccacgtctg cggtgttgtc gagaatggcc ggggcgcacg
    15601 caaatttgtt cccatgggga cgatgcagga cttgatcgga gctaacggca aggcctccgt
    15661 tttctacatg aagctcgacg acccggccaa taccgatcag gtggtcgagg cagtgaagaa
    15721 gcagccgggg atggaaaact acagcgtacg gtccatgcag gaatatctga gcatgatgac
    15781 gccgagcaat ctgcccggct tccgtccgtt catcggcgtg gtgatcggcg tgagtctcgt
    15841 gatcggcttc ctcgtgattt tccagtcgat gtatacggcg gtcatggaac gcacgcgtga
    15901 aatcgggatt ctcaagtcga tgggggcatc aaagtactac atcgtcagcg tggtcctgcg
    15961 cgagaccgtg ctgctggcca tcaccgggat tctggtaggg atcgccgtca gcctgatggc
    16021 gcggatcgga atccagcatc gctggccgct ggtacgcatc gacaagagta cggaatggat
    16081 cattcgcgcg accatcatcg cggttttggg ggcgacgggc ggggctctct atcctgctta
    16141 caaagcggcg cagaaggatc cgattgatgc actggcctat gaatgatggc cttcgcttgt
    16201 tcgattccac gccaacccga tcagcgcttc ttcttcgttg ttggcttcgg tttcagcgtc
    16261 ctggcgtatt cgtagcttaa gtcgagatac ttcttcagct cttttgtatt ggcgagcatt
    16321 tcgtcgggaa cggccacgta ttccttcatg accgtgccgt aggcttcata cagtttcgtc
    16381 ttgtacttct ttagaaactt ctcccgctct tcttccggca accgtatcgc taatcgggat
    16441 tcgtgcagaa gcgtgaacat gttcccgttg accgcggtgt agggattcgc gtcgcccttg
    16501 cgctcgattt cgggattggt ggcgatcagc ttgtcgtaca gcgcttcccg atcgtcagag
    16561 acacttcgat ttcgtgccg
//
LOCUS       DASPUL010000206         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1813101, whole genome shotgun sequence.
ACCESSION   DASPUL010000206 DASPUL010000000
VERSION     DASPUL010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813101"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(407..1114)
                     /gene="phoU"
                     /locus_tag="VEJ00_04900"
     CDS             complement(407..1114)
                     /gene="phoU"
                     /locus_tag="VEJ00_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXX00530.1"
                     /translation="MRTRFQQGLDDLRQRLLRMGGLAEQAVDRARQAYVDRDLTRCQM
                     VLEGESMINMAEREIDEAAFDLLAMQQPMAVDLRFILAVTKINADLERVGDQAVNIAE
                     RVMDMVDLPPADLPVDIARMGAAVSAMVRRALESFIEGKVELAQAVLEMDSVIDRMRD
                     EAFIQLVRTMNERPEMVRQALDALLIARNLERVADHATNIAEDVIFWVRGADVRHNVH
                     PEDEGHETPTTTRASGV"
     gene            complement(1199..1957)
                     /gene="pstB"
                     /locus_tag="VEJ00_04905"
     CDS             complement(1199..1957)
                     /gene="pstB"
                     /locus_tag="VEJ00_04905"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HXX00531.1"
                     /translation="MGVGIHVDNLNAWYGKGQALYDINLKVAANHATALIGPSGCGKS
                     TFIRCLNRMHETIPEARVTGNVRIGEIDAYNGTSPTQLRRRVGMVFQKANPFPTMSIY
                     DNVASGLKLNGFRDRRKLQEIVERSLGAAALWEEVKDHVHKKSGASLSGGQQQRLCIA
                     RALAVEPEVLLMDEPCSALDPISTGKIEELIFQLKERYTIVIVTHNMQQAARVAEFTG
                     FFLLGKLIEFDKTEKIFTNPSDKRTEDYITGRFG"
     gene            complement(1960..2838)
                     /gene="pstA"
                     /locus_tag="VEJ00_04910"
     CDS             complement(1960..2838)
                     /gene="pstA"
                     /locus_tag="VEJ00_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXX00532.1"
                     /translation="MPETAPNTRPVLHISWYRRLTDYVMTGAAILTVILVLAPLVAIF
                     AYLLYKGIGSINWAFLTQIPKPVGEPGGGMANAIVGSVLILAIASVIGVPFGIGAGIY
                     LAEYGRNRLGGFIRFTADVLNGVPSIVVGIVAYAIVVLYQKHFSALAGGVALAIMMVP
                     TISRTTEEMLLLVPQALREASYGLGVPHWRTTLSITLRTATSGVITGIMLAFARVAGE
                     TAPLLFTSLGNEYWNLRIDQPTAAVPLQIFKYAISPYDEWHRQAWAAALVLIVLIVTS
                     IAAVRYVVRRGTFGAA"
     gene            complement(2831..>3196)
                     /locus_tag="VEJ00_04915"
     CDS             complement(2831..>3196)
                     /locus_tag="VEJ00_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXX00533.1"
                     /translation="REGVLALGATRWEMIRVGVLRNARAGIVGAIILGLGRALGETMA
                     VTMVIGNRPEIAKSLFASGYTMASVLANEFSEATGDIYLSALVEIGLALFLVTIVVNA
                     LARLLVWTVTRGQPARGHA"
BASE COUNT          644 a         1031 c          938 g          583 t
ORIGIN      
        1 gctcggaatg acaagccgtg gaagcgcagg aatgacgacg accgagcatt agggaagaca
       61 cgaatcacga ctgcctccga gaaccggtgg aatcagaatg aaaggaagtg accagcgtaa
      121 tgcaaggctg tgcaggcggt caaagcggac ctggctggag ggcgcccacc acgtcgggtt
      181 cgatggcagc ttctcccaag ccattagaca ttccggacgg cgtcatcccg aaggcccgcg
      241 ctttcaccag cgggccgagg gatcccgcga gggctgcatc tagtgcgcgc gagatccctt
      301 cgccttcagc tcagggcagg ctcttcgctc cgcctgaaaa acggctccgc tcaggatgac
      361 gcccccggga agtgctgtta tcaagattta agactggaaa gggcccctac accccggacg
      421 cgcgagtggt ggtcggagtc tcgtgaccct cgtcctccgg atgcacgtta tgccgcacat
      481 cggcgccgcg cacccagaag atgacgtcct cggcgatatt ggtggcgtgg tcggccacgc
      541 gctccaggtt gcgggcgatc agcagcgcat ccagcgcctg ccgtaccatc tcgggccgct
      601 cgttcatggt gcggaccagt tgaataaacg cttcgtcgcg catgcggtcg atgacgctgt
      661 ccatttcaag cacagcctgc gccagctcga ccttgccttc aatgaacgac tccagcgccc
      721 gccgtaccat ggcgctcacc gccgcgccca tgcgggcgat gtcgaccggc aaatcggccg
      781 gcggaaggtc caccatgtcc atgacgcgct cggcgatgtt gaccgcctga tcgcccacgc
      841 gctccaagtc cgcgttgatc ttggtcacgg ccaggatgaa tcgcaaatcg acggccatgg
      901 gttgctgcat ggccagcagg tcgaacgccg cctcgtcgat ctcgcgctcg gccatgttga
      961 tcatgctctc gccttccagc accatctggc agcgggtcag gtcgcggtcg acgtaggcct
     1021 ggcgggcgcg gtccaccgcc tgctccgcca gccctcccat gcgaagcagc cgttgccgca
     1081 gatcatcaag cccttgctga aagcgggtgc gcatgattca aacctttcgt cagggatcag
     1141 gggccaggga ccagaaacgc gcgtgatcta ctgatccctg gcccctggtc gctggtccct
     1201 agccaaacct tcccgtaatg taatcttcgg ttctcttgtc cgaaggattg gtgaaaatct
     1261 tctccgtctt gtcgaactcg atcagcttgc ccagcaggaa aaagcctgta aattcagcca
     1321 cgcgcgccgc ctgctgcatg ttgtgcgtga cgattacgat ggtataccgc tctttgagct
     1381 ggaagatcaa ctcctcgatc tttccggtcg agatcggatc gagcgccgag cagggctcat
     1441 ccatgagaag gacctccggt tcgaccgcca gcgcccgcgc gatgcacaac cgctgctgct
     1501 ggccaccgga gaggctcgcg ccagattttt tgtgcacgtg gtccttgact tcctcccaga
     1561 gcgcggccgc tcccagcgaa cgctcgacaa tctcctgcag cttgcgccgg tcgcggaagc
     1621 cgttcagctt caatcccgag gccacgttgt cgtaaatcga catcgtggga aacggattcg
     1681 ccttctgaaa caccatgccc acgcggcggc ggagttgggt aggtgaagtg ccgttgtagg
     1741 cgtcgatttc gccgatccgc acgttcccgg tgacccgcgc ctcaggaatg gtctcgtgca
     1801 tgcggttcag gcaacggata aatgtcgact ttccgcaccc cgagggcccg atcagcgccg
     1861 tggcatgatt ggcggccact ttcagattga tgtcgtacag cgcctggcct ttaccgtacc
     1921 aggcattcag attgtcgaca tggattccga cgcccatgat tatgccgctc caaatgtacc
     1981 gcgccgcacc acgtaacgta cagcggcgat tgacgtgaca atcaggacga tgagcaccag
     2041 cgctgcggcc cacgcctgac gatgccactc gtcatatggg gaaattgcgt atttaaagat
     2101 ctgcagcgga accgccgccg tgggctggtc aatgcgcagg ttccagtatt cgttgcccag
     2161 cgaagtaaac aagagcggcg ccgtttcacc ggcgacgcgg gcaaaggcca gcatgattcc
     2221 ggtaatcacg ccggaagttg ccgtacgcag cgtgatcgaa agcgtggtcc gccagtgcgg
     2281 caccccgagc ccgtaagacg cctcgcgtag agcctgcggc accagcagca gcatctcttc
     2341 cgtggtccgg ctgatggtcg gcaccatcat gatggccagc gccacgccgc cagccagtgc
     2401 ggaaaaatgt ttctgataca gcaccacgat cgcgtaggcg acgatgccga caacgatcga
     2461 cggcacgccg ttcagcacgt ccgcggtaaa gcggatgaaa ccgcccaggc gattgcggcc
     2521 atattctgcc aggtagatcc cggcgccgat cccgaacggg acaccgatca cgctggcgat
     2581 tgcaagaatc agcacagatc cgacgattgc attggccatg ccgccgcccg gttccccgac
     2641 cggtttggga atctgtgtaa gaaaggccca gttgatcgag ccaatgccct tgtagaggag
     2701 atatgcaaag atagccacca gtggcgccag aaccaagatc acagtcaaaa tggccgcccc
     2761 cgtcatcacg tagtcggtga gacggcggta ccagctgatg tgtagtactg gccgcgtgtt
     2821 cggtgctgtc tcaggcatgt cccctcgcgg gctggccacg cgtcacggtc cacaccagca
     2881 ggcgcgccag cgcattcacg acaattgtca ccaggaaaag cgccaggccg atctccacta
     2941 acgcggaaag atagatatca cccgtggcct cgctaaactc attcgccaga acgctggcca
     3001 tggtgtaccc ggaggcaaac agtgacttcg cgatctcagg gcggttgccg atcaccatgg
     3061 tcaccgccat ggtctcgccc agcgcgcgtc ccagccccag gatgatcgcg cccacgatgc
     3121 cggcgcgggc attgcgcaaa acccctaccc gaatcatctc ccagcgcgtc gcgcccagtg
     3181 ccagcacgcc ttcgcg
//
LOCUS       DASPUL010000207         5100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_857219, whole genome shotgun sequence.
ACCESSION   DASPUL010000207 DASPUL010000000
VERSION     DASPUL010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857219"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VEJ00_04920"
     CDS             complement(<1..308)
                     /locus_tag="VEJ00_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00534.1"
                     /translation="MDRAELRDRAWQEFAKRQDSVLAQLGYGFGREVRRSGARAGNFF
                     FAAEGVEQILTLLRERLPGQAASIVSQAEKICRHRFDLLGYADLDFGRPMDWHLDPVH
                     G"
     gene            752..3331
                     /locus_tag="VEJ00_04925"
     CDS             752..3331
                     /locus_tag="VEJ00_04925"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HXX00535.1"
                     /translation="MAALIAVGATAAHARSDAPGQPATPDSSSISVSVPQQQAKVGTA
                     YNVLPVVKGGSAPYFFGVLAGILPPGLTLNPRTGSISGTPTAAGEFSFTLFASDLRLL
                     EWAITPVRIVVIKGGSGTVSTIKISPTNATIASQGTGQFAATISGTSNTAVLWSSTAG
                     SISPSGRFTAPRVGSRTTITVTATSQQNSSLSASATVVVTPEAAALAITTSSVTGATA
                     GSPYSASFSANGGTLPYHWTIAAGTLPAGIQLQSASGILAGTTSLSGSFHFSVKVSDS
                     AGATAAHDFELVSSASSNQSGNFDGPAELPRTLIQTAMSDTPAPGSSIAVKSGSDLQS
                     ALDRASCGDTLELQAGATFTGQFTFPARSCDDNHWIIVRTSASDAALPPEGSRLTPCY
                     AGVASLPGRPALNCASTANVLAKLVMPTSAVGPIVFASGANHYRFVGLEITRTPGTGI
                     VYSLASLCKSGTINNVIFDRVWLHGSAQDETKRGVELAGASFVSVVDSSLTDFHCTAS
                     SGACTDAAAIAGGQGNPVGPFKIVNNFLEASGENILFGGAQSATTPSDIEIRQNHFFK
                     PLTWMRGQPGQVSATDGNAFIVKNFLELKNAQRVLVEGNIMENTWGGFSQKGYAIVLT
                     PKNQASGGGNICPNCLVSDVTIRFNTISHVGGGLQIANALSDNGGVALDGERYSIHDV
                     TIDDIDLKKYNGTGHFAEVETDRGAPLVRSVAINHVTAFPPSGLFTIGGDPQAKMSDF
                     SFDNSLVTVGSSPVWSIGGGTANCAFYDKPLTTFTACFSQYSFTNNALIATPPEYPLS
                     AWPRGNAFPASISAVGFVNYNGGNGGDYHLLPSSPFKNAGTDGKDLGADIDAIQAATA
                     GVY"
     gene            3516..4271
                     /locus_tag="VEJ00_04930"
     CDS             3516..4271
                     /locus_tag="VEJ00_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HXX00536.1"
                     /translation="MDIAHAAFRIEAPRQLAADQVHLWRVDLQAIAGEEPQWAAVLSP
                     DEQARAARFHFQEHRRYFTASRAILRHVLSAYLGCNPKNVAFVYSSKNKPALGGAHAG
                     ALAFNVSHSGDIALLAIARSRPVGVDIELIRHNFDTAAIAARFFSDAEQKQLAALPPD
                     QRHDAFFRCWTRKEAYIKALGEGLSLPLSQFDVSLTPDDGNALIATRPDALEARRWSL
                     REVRVKPGYAAALCVQGTDWTLIDWNATPGRTP"
     gene            4271..5068
                     /locus_tag="VEJ00_04935"
     CDS             4271..5068
                     /locus_tag="VEJ00_04935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXX00537.1"
                     /translation="MRASGVVFLMYHELELEGRSLCQTDPGYVRYVVRGSDFRTQMQL
                     LSSDGWSGSSVGEAIASFPERAVAITFDDGSETDLLVAAPILSEFGFTATFYITSGWL
                     GRPGFLAPSQLCELSSLGFEIGSHSMTHAYLTDLEEVDMRREISDSKLQLEQILGKPV
                     EHFSCPGGRFDARVARVAREAGYRTVSTSEPRINTPATNRFALGRVAVLRHNSPSELL
                     GVCMGRGLSRSRIGARLREATRSALGNSTYDRLRNTLLRRPKDDGQR"
BASE COUNT          956 a         1719 c         1406 g         1019 t
ORIGIN      
        1 ccatgaaccg ggtcgagatg ccagtccatc ggccggccga aatcgagatc ggcgtatccc
       61 agcagatcga aacgatgccg gcagatcttc tccgcctgcg acacgatcga cgccgcttgt
      121 cccggaagtc gctcgcgcag gagggtaaga atctgctcaa cgccctccgc cgcgaagaaa
      181 aagttacctg ctcttgcccc tgacctccgc acctcgcgcc caaacccgta ccctaactgc
      241 gcaaggaccg aatcctgccg cttggcaaac tcctgccacg cgcgatcgcg gagttccgcc
      301 cgatccatcc gagtcaattt ctggccgatt tgttgccatc tgccaagccg catggtgtca
      361 gtgcgtcact ctcgccaagc cgcgaaaagg gctacgatct tccatcttaa caatcctgta
      421 gcacctgtct cgtttcttca tgccacgaaa ccggacagtg cctctcgagc cgagggaaag
      481 ccccccggtt acccggttgc ccgtcagcgg tttccaccgc gcgaggtaca ttgaccttcc
      541 gacatgtact agtgtgacca gctagaccct gatcccacgt tatctaatca atctcgtggc
      601 ttctctttac agttctctca ggactcgaac gatggtcccc caactccgtt ccgaggttgt
      661 ccccttgacg cgtcgtgtcg cagcgcaaat ctcaatccgc actgttctta aggctctttc
      721 atcttcgcat ctgctgacta ccgcgggagc cttggcggct ctgattgccg ttggcgcaac
      781 cgcggcacac gcgcggagcg acgcgcccgg ccagcccgca accccggaca gcagctccat
      841 ttctgtttcc gtgccgcagc agcaggccaa ggtcggaacg gcttacaacg tcctgccggt
      901 ggtaaaagga gggagcgcac cctacttctt tggcgtgctg gctggtattt tgcctcctgg
      961 gctgacgctc aatccgcgca ccggttcgat cagcggcacc ccgacagcgg caggtgaatt
     1021 cagcttcacg cttttcgctt ccgatcttcg cctcctcgaa tgggccataa cgcctgtccg
     1081 gattgtggtc atcaagggag gctccggaac tgtttccacc atcaagatct cgcccacgaa
     1141 tgcgacgatc gcctcgcagg gaaccgggca attcgcggcc accatcagcg ggacttccaa
     1201 taccgcagtg ctctggagtt cgacagccgg cagcatctct ccctcgggca ggttcactgc
     1261 cccgcgtgtg ggtagccgga cgacgattac ggtcaccgcc acgagccagc agaacagcag
     1321 tcttagcgcg tcggcaacgg tcgtagtaac tcccgaagcc gcggcgctcg ccatcacaac
     1381 atcgtctgta accggagcca cagccggatc gccctacagc gcttccttct cggcgaatgg
     1441 cggcacactg ccctaccact ggaccatcgc ggcaggaacg ctgcccgcgg gaatccagct
     1501 gcagtccgcc agcggcatcc ttgccggaac caccagcctc agcggctcgt ttcacttctc
     1561 ggtaaaggta agcgactccg cgggcgcgac cgccgcacat gacttcgaac tggtgtcgtc
     1621 ggcttcttcg aaccagagcg ggaatttcga cggcccggca gagttgcctc ggactttgat
     1681 ccagaccgca atgtccgaca caccggctcc cgggtcgtcg atcgcagtga agtccggcag
     1741 cgatctgcag tctgcccttg accgcgccag ttgcggcgat accctggagt tgcaggccgg
     1801 tgcaaccttc accggacaat tcacgttccc ggcccgaagc tgcgacgaca atcactggat
     1861 tatcgtacgc accagcgcca gcgatgccgc gctgccgccc gagggcagcc gcctgactcc
     1921 gtgctacgcc ggggttgctt cccttcccgg tcgccctgcc ctgaattgcg cctcgacggc
     1981 gaacgttctg gccaagctgg tcatgcccac atctgcggtt ggcccgatcg tcttcgcatc
     2041 gggcgcgaat cactatcgtt tcgtgggcct cgagatcacg cgcaccccgg gcacgggaat
     2101 cgtctactct ctggcatctc tctgcaagag cggcacaatc aacaacgtga tttttgatcg
     2161 tgtctggctg catggctcgg ctcaagacga gaccaagcgc ggcgtcgaac ttgccggtgc
     2221 atccttcgtg agcgtagtgg actcctcctt aaccgatttc cactgcacgg cgagttcggg
     2281 cgcctgcact gatgccgcgg ccatcgccgg aggacaaggc aatcccgtcg gccccttcaa
     2341 gatcgtgaac aacttcctcg aagcttcggg agaaaacatc ctgttcggcg gtgcccagag
     2401 cgccaccacg ccgtccgaca tcgagatccg acagaaccac ttcttcaagc ctttgacctg
     2461 gatgaggggt caacccggcc aggtcagcgc taccgacgga aatgccttca tcgtgaagaa
     2521 ttttctggag ctcaaaaacg cccagcgcgt gctggtcgaa ggcaacatca tggagaacac
     2581 ctggggaggg ttctcgcaga aaggctatgc catcgtttta acgccaaaga accaggcgtc
     2641 gggcggcggc aacatttgcc ccaactgcct cgtctcggat gtgaccatcc gcttcaacac
     2701 gatcagccac gtaggcggcg ggctgcaaat cgccaacgcc ttgtctgaca acggaggagt
     2761 cgccctggat ggcgagcgtt acagcattca tgacgtcacg atcgacgaca tcgacctcaa
     2821 aaagtacaac ggcaccggcc atttcgcaga agtcgaaacc gaccgcggcg caccgctcgt
     2881 gcgcagcgtg gccatcaatc acgtcacggc tttccctccg tctgggttgt tcaccatcgg
     2941 tggcgacccg caggcaaaaa tgtcggattt cagcttcgat aacagcctgg tgacagtcgg
     3001 ctcctctccc gtatggtcga ttggcggtgg aaccgcaaac tgcgccttct atgacaagcc
     3061 gctgaccacg ttcacggcct gtttcagcca gtattccttc accaacaacg cattgattgc
     3121 cacccctccc gagtatccgc tcagcgcctg gccccgcggc aacgcgttcc ctgccagcat
     3181 cagcgcggtc ggattcgtga actacaacgg tggcaatgga ggcgattacc acctgcttcc
     3241 ctcgagcccg ttcaagaacg cgggtacgga tggcaaagac ctgggcgctg acatcgacgc
     3301 catccaggcc gcaacagccg gggtctactg atcccggcga agtcacccct ggagaacact
     3361 tcctcgcgcc agccgcgccc gtagccgaaa tttcctttgc taacgcgact ccccggcccc
     3421 tggcggtgtg agccgctgac gtctcactga tacactgtgt acaactggtc tggactgggg
     3481 cccatcgcgg ggacgaggat ccgcgtttct gtttcatgga catcgcacac gcagccttcc
     3541 gcatcgaggc cccgcgtcag ctggcggccg accaggtgca cctgtggcgt gtggatctgc
     3601 aggcaatcgc cggcgaggag ccgcaatggg cagcagtcct gtctcccgat gagcaggctc
     3661 gcgccgcccg ctttcatttt caggaacacc gtcggtattt cacggcttcc cgcgccatcc
     3721 tgcggcatgt gctttccgca tacctgggct gcaatcccaa aaatgtggct tttgtctatt
     3781 ccagcaagaa caagcctgcc ctgggtggcg ctcacgccgg tgcgctcgcc ttcaacgttt
     3841 cgcattcggg agatattgcc cttttggcca tcgcgcgctc tcggccggtt ggggttgata
     3901 ttgagctgat ccgccacaac ttcgataccg ccgctatcgc cgcccgattc ttctccgatg
     3961 ccgaacagaa gcaactcgca gcgcttccgc ccgatcaacg ccacgacgcc tttttccgct
     4021 gctggacgag aaaagaggcc tacatcaagg ccttgggaga aggcctgtcg ctcccgctca
     4081 gccagtttga cgtctcgctc acccccgacg acggcaacgc cttgatcgcg acccgtcccg
     4141 acgcgctcga agcccgccgc tggtctctgc gcgaggtgcg cgtcaagccc ggctatgccg
     4201 cggccctttg cgttcaggga actgattgga ccctcatcga ctggaatgca actcccgggc
     4261 ggacaccctg atgcgcgcat ccggcgtggt ctttctcatg taccacgagc ttgaattgga
     4321 aggccggtcc ttgtgccaga ccgacccagg ctacgtgcgc tatgtggttc gcggctccga
     4381 cttccggacg cagatgcaac tgctcagcag cgatggttgg agtggatcca gcgtgggaga
     4441 ggccatcgcc tcatttcctg agagagcagt tgccattacc tttgacgacg gatccgaaac
     4501 cgatcttctt gtcgccgcac ccatcctctc cgagttcggt ttcacggcga cgttttacat
     4561 caccagcgga tggctgggcc gccccggctt tcttgccccc tctcaactct gcgagctgtc
     4621 ttcgctcgga ttcgagatcg gctctcactc catgacccat gcctacctta ccgatctcga
     4681 agaggttgat atgcgccggg agatttccga ttcgaaactc cagttggagc agatcctcgg
     4741 caaaccggtc gagcacttct cctgccccgg aggccgcttc gatgcgcgcg tcgctcgagt
     4801 cgctcgcgaa gccgggtatc gcactgtttc gacgagcgag cctcgcatca atacccccgc
     4861 cacgaaccgt tttgcgctgg gccgcgtcgc cgtgttgcgg cacaactcgc cttcagaact
     4921 cttgggcgtc tgcatggggc gggggctctc gcgttccaga attggcgctc gcttgcgcga
     4981 ggctacgcgg agcgcgctgg ggaactcaac ctacgatcgc ctgcggaata ctctgctgcg
     5041 ccgccccaaa gacgacggcc agcgttagtg tcacaatatt cagcggacca gattctgcaa
//
LOCUS       DASPUL010000208         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_718170, whole genome shotgun sequence.
ACCESSION   DASPUL010000208 DASPUL010000000
VERSION     DASPUL010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718170"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..2259)
                     /locus_tag="VEJ00_04940"
     CDS             complement(31..2259)
                     /locus_tag="VEJ00_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXX00538.1"
                     /translation="MSGAKLSSSREPAVELRETGQPRAAVPTWPVEPERYELTAGAPY
                     RFDLDRREFFKFLGAGVLVVSVLKPAVVAQESGGTRHQHGDLPKEIDAWLHLGENGKV
                     TVYTGKVEVGQNIRTSLTQAVAEELHVTMEKIEMVMGDTQLTPYDAGTFGSRTTPTMN
                     LQLRRVAAAARDTLLELAAKQWSTKAGQLVAADGKVTDPQTKRSVEYAALTKGQQLTQ
                     MLPAEDPLIPASQWKVAGQSAPKVDGREFVTGKHRYPSDQKLPGMWYGKVLRPEAFGT
                     TLGYLDTTKAEQIGAVVVHDGNFVGVAAPTTAQAEAALGAIEAKWKLEPQVSNTVSNK
                     DLFESLKKNAEQGNDPSGDGDRFDAGDVDKALEAADHKLQATYTVAYIAHTPLEPRAA
                     LAKWDGDQLTVWTGTQRPFGVRGQLAEAFRIPEDHVRVLMPDTGSGYGGKHTGETAIE
                     AARLARAAKRPVKVVWTREEEFTWAYFRPAGVIDVTSGVRADGTITAWEFHNYNSGSA
                     GIRTYYEVPNQRIIFHETNSPLRQGSYRALAATANHFARESHMDEVAHAVKMDPLEFR
                     LKNLKNERLRAVFEAAAKQFGWGKSKGDGQGFGMGGGYEKLGNVATFAEVHVDHKSGD
                     VKVVRVVSAFECGAIVNPDNLRNQIAGSNVQGLGGALFEAIRFENGKILNGRLSQYRV
                     PRFSDVPVLETVLLDRKDIPSVGAGECPLIGLAPAIGNAIFDATGVRLRALPMVPNGL
                     KS"
     gene            complement(2256..2747)
                     /locus_tag="VEJ00_04945"
     CDS             complement(2256..2747)
                     /locus_tag="VEJ00_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXX00539.1"
                     /translation="MATIHLAINGQSYSVDADPQTSLLTVLREHLDLTGSKYGCGEGM
                     CGACTVLIDGRAQRSCITRVGSVAEKRITTIEGLAHGEELHPVQAAFLDAGAMQCAYC
                     TSGMIMSAVALLQKNSHPTQSEIVDFMDGNVCRCGTYPRIVSAIQQAATVMGKSAEKG
                     AGL"
BASE COUNT          529 a         1028 c          737 g          575 t
ORIGIN      
        1 cttggggctt tagcccccgt ggcgcgaatc tcaactcttc agtccattcg gaaccatcgg
       61 tagagctcgc aacctgaccc cggttgcgtc aaagatcgcg ttcccgatcg ccggcgccaa
      121 tccaatcagc ggacactccc ctgcccccac cgaaggaata tccttgcggt caagcaacac
      181 cgtctccaat accggaacat cactgaatcg cggcacgcga tactgcgata gccgtccgtt
      241 caggatcttg ccgttttcga accgaatcgc ctcaaacagg gccccgccca gcccctgcac
      301 gtttgatcct gcaatctgat tgcgcaggtt gtccgggttc acaatcgccc cgcactcgaa
      361 cgccgacacc acgcgcacca ccttcacatc gccggacttg tgatcgacat gaacctcagc
      421 aaatgtcgcg acattcccca atttctcgta gccgccaccc atcccgaagc cctggccgtc
      481 gcccttgctc ttaccccaac caaattgctt cgctgccgcc tcaaacaccg cgcgcaggcg
      541 ctcattcttc aaattcttca gccggaactc cagcggatcc atcttcaccg catgcgccac
      601 ctcgtccata tgcgattcgc gcgcgaaatg attcgccgtc gccgccagtg cccggtacga
      661 tccctgccgc agcggcgaat tcgtctcgtg aaaaatgatg cgctggttgg gaacctcgta
      721 gtacgtgcgg atccccgccg atcccgagtt gtagttatgg aactcccacg ccgtgatcgt
      781 cccgtcggcc cgcactccgc tcgtcacgtc aatgacgcct gccggacgaa aatacgccca
      841 ggtaaactcc tcttcccgcg tccacacgac tttcaccgga cgcttcgccg cccgcgccag
      901 ccgcgccgcc tcgatcgccg tttctccagt atgcttgccg ccatatccgg agcctgtatc
      961 cggcatcagc acgcgcacat gatcttcggg tatgcgaaac gcctcagcca gttgcccgcg
     1021 gacgccgaag ggtcgctgcg tgcccgtcca tacggtaagt tggtcgccgt cccacttcgc
     1081 cagcgccgcc cgcggttcga gcggcgtgtg cgcgatgtag gcaaccgtgt acgttgcctg
     1141 cagcttgtga tcggcagcct ccagcgcttt atcgacgtct ccggcatcga agcgatcgcc
     1201 gtcgcccgac ggatcgtttc cctgctccgc atttttcttt aacgactcaa acaggtcttt
     1261 attcgaaact gtgttcgaaa cctgcggctc cagcttccat ttcgcttcaa tcgcacccaa
     1321 cgctgcttcc gcctgcgcgg tcgtcggcgc cgccacgccg acaaagtttc cgtcgtgcac
     1381 gaccaccgcg cctatctgct cagcttttgt cgtatccaga taaccgagcg ttgtcccgaa
     1441 cgcctccgga cgcagcacct tgccgtacca catgccgggc agcttctggt ccgacggata
     1501 gcgatgcttc ccggtgacaa actcgcgccc atccaccttg ggcgccgact ggcccgccac
     1561 cttccactgc gacgcaggaa tcagcggatc ttccgccggc agcatctgcg tgagctgctg
     1621 tcccttcgtc agcgctgcat attccaccga acgcttcgtc tgcggatcag tgacctttcc
     1681 gtccgcagcc acaagctgac ctgcctttgt actccactgc ttcgccgcca actcgagcag
     1741 ggtatcgcgc gccgccgctg caacccggcg cagttgcaga ttcatcgtcg gcgtcgtgcg
     1801 gctgccgaac gtgcccgcat cataaggcgt gagctgcgtg tcgcccatga ccatctcgat
     1861 cttctccatg gtgacgtgca gttcttcggc cacggcctgc gtcagcgacg tccggatgtt
     1921 ctgccccacc tcgaccttgc ccgtgtagac ggtgaccttg ccgttctccc cgagatgcag
     1981 ccaggcatca atttccttcg gtagatcgcc atgttgatgc cttgtcccgc cagactcctg
     2041 ggcaacgacc gcgggcttca gcaccgacac caccagcacg cccgcaccca gaaacttaaa
     2101 aaactcacgc cggtcgagat caaaccgata aggagccccc gcagtgagct catagcgttc
     2161 aggttccaca ggccatgtag ggacagccgc cctcggctgt ccggtctcgc gcagctcgac
     2221 agccggttcg cgcgaagatg aaagtttcgc cccgctcata acccggctcc cttctcggcg
     2281 gacttcccca tcaccgtcgc cgcctgctga atcgcgctca cgatccgcgg atacgtcccg
     2341 caccggcaga cattgccgtc catgaaatcc acgatctcgc tctgcgtggg atgcgagttc
     2401 ttctggagca gcgcgaccgc cgacatgatc atccccgaag tgcagtaggc gcactgcatc
     2461 gcacccgcgt cgagaaacgc cgcctgcacc ggatgcagct cttccccgtg cgccaaccct
     2521 tcgatcgtag tgatcctttt ctctgctacc gacccgacac gcgtgatgca tgaacgctgc
     2581 gccctcccgt cgatcagcac ggtgcacgcg ccgcacatgc cttccccgca gccatacttg
     2641 ctgccggtca ggtccagatg ctcgcgcaag accgtcagca ggctggtctg cggatcggcg
     2701 tcaaccgagt aagactgtcc gttgattgcg agatgaatgg tagccatgcg caacctccat
     2761 cgacgaagag gaatccgagc ggagacgttg gcagatgctt ctgaaggaaa tgatataccc
     2821 gcagcgcgga ggtagtaacc tacgagagca gagggggttt cgccagttg
//
LOCUS       DASPUL010000209         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_718281, whole genome shotgun sequence.
ACCESSION   DASPUL010000209 DASPUL010000000
VERSION     DASPUL010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718281"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VEJ00_04950"
     CDS             <1..738
                     /locus_tag="VEJ00_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00540.1"
                     /translation="ELLHKGGKKMAFAGVFARPPKNSHEVLQPDAYINGEKLPPVAIP
                     DLKQLLGNDYDVYDSGGIGQLDVRALLKQFGERKIADDLASAWQGGAYVTFHRKDKPA
                     PAATTADLALLYVSRWKTPQAAEHFARFYTGAVSQRYRSATAQPAPACAGSSCPVASA
                     QIATEEGPVIVEQWADNSVVVSESFDSSMAAKIQSALRDGSAGAHAANYSQEEIGLGL
                     FDVPAFRAFAEGIGAKIAQEIVRNSGR"
     gene            860..1336
                     /locus_tag="VEJ00_04955"
     CDS             860..1336
                     /locus_tag="VEJ00_04955"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXX00541.1"
                     /translation="MADFTLRVNGSEKKISVSPETPLLWVLRDTLELTGTKFGCGAGL
                     CGACTVHVDGGAVRSCSTPVSQVAGKSVTTIEGLGANGLHVLQQAWIAEEVPQCGYCQ
                     TGQIMAAAALLAKMPNPTDEQITQAMNGNLCRCGTYERIRKAIHRAASSAGSGGTR"
     gene            1333..>2021
                     /locus_tag="VEJ00_04960"
     CDS             1333..>2021
                     /locus_tag="VEJ00_04960"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXX00542.1"
                     /translation="MSPLSRREFVAAGVAAGAGLVIGFYLPHGRSENELFSPNAYLRI
                     TPDNKVTIVVARSEMGQGVRTALPMILAEELEADWKQINIEQAGASTLFGDQTTGGSA
                     SVRTTWDPMRKAGAQAREMLISAAALTWGVPRSACSAESGHIKHAASGRSLSYGELVG
                     KAATLPIPTDVPLKQSKDYKIVGQRLPRVDSPAKVKGEAEFGIDVRMPGMKFAVLSRC
                     PVIGGKVASLD"
BASE COUNT          395 a          639 c          638 g          349 t
ORIGIN      
        1 gagctgttgc acaagggcgg caagaagatg gcatttgccg gggtcttcgc gcgcccgcct
       61 aaaaattcac atgaggtgct gcagcccgat gcctatatca acggcgaaaa gctgccgccg
      121 gtcgcgatcc ccgatttgaa gcagctactg ggcaacgact acgacgtcta cgactcgggc
      181 ggcatcggcc agctcgatgt gcgcgccctg ctgaagcagt tcggcgagcg gaagattgcg
      241 gacgatctcg cttccgcctg gcagggcggc gcttacgtga ccttccatcg caaagacaag
      301 cccgctccgg cagcgactac ggccgacctg gctcttctct acgtctcgcg ctggaagacg
      361 ccgcaggccg ccgaacattt tgcgcggttc tacaccggcg cggtcagcca gcggtatcgc
      421 agcgctacag cgcaaccggc tccggcttgt gccggatcta gttgcccggt agccagcgcc
      481 cagatcgcga ccgaagaagg cccggtgatc gtggagcagt gggcagacaa cagcgtggtg
      541 gtctccgaga gctttgacag cagcatggcc gccaagatcc agagcgcgct gcgcgacggc
      601 agcgcaggcg cgcacgctgc aaactattcc caggaagaaa tcgggctagg cctcttcgac
      661 gtcccggcct tccgcgcgtt tgccgaaggc atcggcgcga agatcgcaca ggaaatcgtg
      721 cggaactccg gccgctgacc ccccgctaca tccgatccga cggccagtgg gctggtccca
      781 acgcaacacc ctttaccatt cccctttttc ttggagttga gtacgatgtt ggtccagagc
      841 gaatggctct ggcaccgaca tggccgactt cacacttcgg gttaacggca gtgaaaagaa
      901 aatcagcgtc tcgcccgaga cgcccctgct ctgggttctg cgcgacaccc tggaattgac
      961 gggaaccaag ttcggctgcg gcgccggatt gtgcggcgcc tgcaccgttc atgtggacgg
     1021 cggggcagtg cgctcctgct cgaccccggt ttcacaagtc gccgggaaaa gcgtgaccac
     1081 gatcgaaggc ctcggcgcca acggcctgca cgtgctgcag caggcctgga tcgccgaaga
     1141 ggttccgcaa tgcggctact gtcagaccgg ccagatcatg gccgccgccg ctctgctggc
     1201 caaaatgccc aatccgaccg acgaacagat cacgcaggcc atgaacggca atctctgtcg
     1261 ctgtggtacg tatgagcgga tccgcaaggc gattcaccgc gctgcatctt cggccggaag
     1321 cggagggact cgatgagccc gctttcccgc cgtgaattcg ttgctgccgg agttgcggcc
     1381 ggcgccgggt tggtcatcgg tttctatctg ccgcacggcc gctcggagaa cgagttgttt
     1441 tcccccaacg cgtacctgcg catcacgccc gacaacaagg tcacgatcgt tgtggcgcgc
     1501 tcggagatgg gacagggcgt gcgcaccgcg ctgcccatga ttctggccga ggaactcgag
     1561 gccgattgga agcagatcaa tatcgaacag gcaggcgcga gcaccctctt cggcgatcag
     1621 acgacgggcg gaagcgccag cgtccgaacc acgtgggatc ccatgcgcaa ggccggagct
     1681 caggcacgcg agatgctgat ctcggcggcg gctctgacct ggggcgtgcc gcgatcagcc
     1741 tgcagtgcag aatccggcca catcaagcat gctgcatcag ggcgaagctt gagttatggc
     1801 gaacttgtcg gtaaagctgc gactttgccc atcccaaccg atgtcccgct caagcagagc
     1861 aaggactaca agatcgtcgg acagcgcctg ccgcgcgtgg actcgcccgc gaaagtcaaa
     1921 ggcgaagccg agtttggaat tgacgttcgc atgcccggca tgaagttcgc ggtgctgtcg
     1981 cgctgccctg taatcggcgg caaggttgcg agcttggacg a
//
LOCUS       DASPUL010000210         2755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_445100, whole genome shotgun sequence.
ACCESSION   DASPUL010000210 DASPUL010000000
VERSION     DASPUL010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445100"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VEJ00_04965"
     CDS             <1..270
                     /locus_tag="VEJ00_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00543.1"
                     /translation="TMDISAIPIIHKLSHLPMTADPSHGTGRRDKVAPMARASVAAGA
                     DALLIEVHCNPDKAWSDGAQSLFPEQFSKLMDELRIIAPAVGRKI"
     gene            375..1241
                     /locus_tag="VEJ00_04970"
     CDS             375..1241
                     /locus_tag="VEJ00_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HXX00544.1"
                     /translation="MPIRQITIIGTGLIGGSLALALRRKKFAGRIVGCDREGTLERAR
                     MRGAIDDGTVNPGDAVRGSQLVVLATPVLAIVDLIERVGPALPAKALLTDVGSTKAAV
                     VERAVKVFGKSAGKKFLAGHPMAGKELSGVDYADADLFQNAVWFLTPLPGQSLNEGLF
                     AEFAGWIDAIGARIAMLPPDEHDRLCAWISHVPQMISTALAAALVEEFGPEAPLLAAG
                     GRALQEMTRISASPYSMWRDVAISNKKNLEDALSKVEQRLAHLRENLATRQLAQEFEQ
                     AHQLRKAPSKKR"
     gene            complement(1264..1452)
                     /locus_tag="VEJ00_04975"
     CDS             complement(1264..1452)
                     /locus_tag="VEJ00_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00545.1"
                     /translation="MSPIAEFDQRLEQMIREFAIRILVPRWTAFDAVWETANRICDRY
                     ELGKHISEPRPANSPKLA"
     gene            1732..2433
                     /locus_tag="VEJ00_04980"
     CDS             1732..2433
                     /locus_tag="VEJ00_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HXX00546.1"
                     /translation="MNKVVANADEAIRDVFDGATIMIGGFGLCGMPENLIRALVRKGT
                     KNLRTISNNVGVDGVGNGLLLAAGQIVHHIGTYVGENKLLEEMVLQGRIKLDLVPQGT
                     FSERIRAGGAGIPAFYTPTGVGTVVAEGKETRDFEGRLFVMERGLTADFAFVKAWKGD
                     RAGNLIYRKTARNFNPMMATAAKITIAEVEHLVEVGELDGDSIHTPSIYVKRIFQGEL
                     YQRRIERRTVRKAAS"
     gene            2576..>2755
                     /locus_tag="VEJ00_04985"
     CDS             2576..>2755
                     /locus_tag="VEJ00_04985"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HXX00547.1"
                     /translation="MTRPAKDTDKRERIVKRIARELRDGFYVNLGIGMPTLIANHVPP
                     GMEVTLQSENGMLGIG"
BASE COUNT          571 a          761 c          868 g          555 t
ORIGIN      
        1 accatggaca tttctgcgat cccgatcatt cacaagctgt cgcacctgcc catgacagcg
       61 gatccttcgc acggaacggg ccgccgcgat aaggtcgccc ccatggcgcg ggcgtcggtc
      121 gccgccggag ccgatgcgct gctgatcgag gtgcactgca atcctgacaa agcatggtcg
      181 gatggcgcac agtcgctgtt tcccgaacag ttctcaaagc tgatggacga gctgcgaatt
      241 atcgcgccgg ccgtgggaag gaaaatctag cattttgtaa tttggtaatc gggtaatttt
      301 ccaatctaag ccctcttggg tattcgcttt ctagatttcc cgattacaaa attacccaac
      361 tacaaaatct ttccgtgccc attcgccaaa ttacgattat cggtaccggc ctgatcggcg
      421 ggtcgctggc tctggcgctg cgcagaaaga aattcgccgg ccgtattgtc ggatgcgacc
      481 gcgagggcac gctggaacgc gcccgcatgc gtggcgcgat tgacgatggc acggtcaatc
      541 ccggagacgc ggtgcgcgga agccagctcg tggtgctggc tacgccggtg ctggcgattg
      601 tcgatctcat cgaacgagtt ggcccggcac ttcccgcgaa ggcattgctc accgacgtgg
      661 gaagcacgaa ggctgctgtc gtggaacgag ccgtgaaggt gttcggcaag agcgccggga
      721 agaaatttct ggcgggacat ccgatggccg gcaaagagct gagcggagtt gactacgccg
      781 atgccgatct gtttcaaaat gcggtgtggt ttctaacgcc gctccccgga cagagcttga
      841 acgagggtct ctttgccgag tttgcgggat ggatcgatgc gatcggagca cgtatcgcga
      901 tgctgccgcc ggacgagcac gaccgcctgt gcgcctggat cagccacgtc ccgcaaatga
      961 tctcaacggc gctggccgcg gcgctggttg aggagtttgg tcccgaagct ccgctgcttg
     1021 ccgccggcgg ccgcgcgctg caggaaatga cccgcatctc cgccagcccg tattcgatgt
     1081 ggcgcgatgt cgccatcagc aacaagaaga acctggaaga cgcactgtcg aaggtggagc
     1141 agcgcctggc gcatctgcgg gagaacctgg caactcgtca actggcgcag gaatttgagc
     1201 aggcccacca gctgcggaag gccccgtcaa agaagcggta agcttcggcg gcgggagtcc
     1261 ggactacgca agtttcggag aattcgccgg tcggggctcg gatatgtgct ttcccagttc
     1321 gtaccggtcg cagatccggt tggcggtctc ccagacggcg tcaaaagcgg tccagcgcgg
     1381 aacgaggatg cgaatggcga actcgcggat catctgttcc agtctctgat cgaattcagc
     1441 gatgggggac atgttcgctc gccgtgcccg ggcttgcaac cgggctgccg cattgcacgg
     1501 gagaaggttc gctcccgtgt gctctgcgtc cagcatctcc gactactttt acaaccagag
     1561 caagctgcac tcgcgaaagc tgctttcttt cgttctgcat ggtttgacgc ggctgacatg
     1621 cgtttggatg cgccgccctg tcatcagatt gtcaaatgat tgtctgcacc atcgcacggg
     1681 aaatactgta gagagtgtct ccgctatgga tttcatttag aattctcccg catgaataag
     1741 gtcgtagcca atgccgacga ggccatccgc gacgtgttcg acggcgcgac catcatgatc
     1801 ggtggcttcg gactctgcgg catgcctgag aacctgatcc gggcactggt gcgcaagggc
     1861 acgaagaatt tgcgcaccat cagcaacaac gtcggcgtag acggagtggg caacggcctg
     1921 ctcttggcgg cggggcagat cgtgcatcac atcgggacct acgtcggcga gaacaagctt
     1981 ctcgaggaga tggtcctgca gggcaggatc aagcttgatc tcgttcccca gggcacgttt
     2041 tccgagcgca tccgtgccgg cggcgcgggg attcccgcgt tctacacgcc gactggagta
     2101 ggaaccgtgg tcgccgaggg aaaagagacc cgtgatttcg agggccgctt attcgttatg
     2161 gagcgcgggc ttacggctga ctttgctttt gtgaaggcgt ggaaaggcga ccgggccgga
     2221 aatcttatct atcgcaagac ggcgcggaat tttaatccga tgatggccac cgcggcgaaa
     2281 atcacgatcg cggaagtcga gcatctggta gaagtcggtg aactggatgg cgattcgatt
     2341 cacacaccgt cgatttatgt gaagagaatt tttcagggcg agttgtacca gcgaaggatc
     2401 gagcggagaa cggtgaggaa agcggcctcg taacgctgct gtcaagtaga gcaattgtgg
     2461 ctgtgcaggc tgttgtcatt ctgagcgaag cgaagaaccc atgcattttc ctggcgcccg
     2521 gcagagtgca tggatccttc gcttcgctca ggatgacaat gcactaaggg cgtaaatgac
     2581 caggccggca aaagacaccg acaagcgcga gcgaatcgtc aagcgcatcg cgcgcgaact
     2641 gcgcgacggt ttctatgtga acctgggcat cggcatgccg acgctgattg ccaatcacgt
     2701 accgccaggc atggaggtca cgctgcagtc ggagaacggc atgctgggga tcggc
//
LOCUS       DASPUL010000211         8340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_37783, whole genome shotgun sequence.
ACCESSION   DASPUL010000211 DASPUL010000000
VERSION     DASPUL010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8340)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37783"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /gene="rapZ"
                     /locus_tag="VEJ00_04990"
     CDS             <1..732
                     /gene="rapZ"
                     /locus_tag="VEJ00_04990"
                     /inference="COORDINATES: protein motif:HMM:NF003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HXX00548.1"
                     /translation="FAELSMQSSESRRTALVVDVREGAKLEQLPGILKSVRRMIPTKV
                     VFLEASDAVLLRRFSETRRPHPLGTDAPVKAALKAERRHLGAIRRLADYVIDTSKFNV
                     HELRAHITERFQEQSSEKSILVSIVSFGFREGVPEDADLVFDVRFLPNPHFVPEFRPL
                     TGRDPRVARYIRSFPQTREFIARISDLLVYLLPHYIHEGKAYLTIAFGCTGGQHRSVM
                     IAEEVAKHLRRADYRVKVVHRDSPK"
     gene            979..2811
                     /locus_tag="VEJ00_04995"
     CDS             979..2811
                     /locus_tag="VEJ00_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXX00549.1"
                     /translation="MRLVRYVLPFLLQLLPGVLLLAAVGFLEAFRLMLLKPVLDRVLN
                     PASGSENIILFTWPATGEPIYLQRFVPTHFHNAWTIVAYALVASTVLKGVFDYAGTYL
                     VNHAGFGMITNLRNDLYNSVLQRSAAFFQKHTTGTLISTIINDIERVQFAMSSILAEF
                     LQQVFTFAFIAVAVVILGRQLAWVLVLFVPFIIYSAVRIGRRVRTTTRRGQDQLADVQ
                     NILHETITGNRIVKAFGMESWEVARFRKAAQRLFRANLRSVAAAAISSPLMDVFGAIA
                     IALLLLLGRDRIAHNEMTLGAFVTFVAAVLSMYNPVRKFAVFNNSFQQALGASSELFK
                     FIDAEDVVRDKAGAKPLPRFSESIRFEDVFFKYEGDGDEAREVLRGIDLEVKRGEVLA
                     VVGSSGAGKTTLVHLIPRFFDVSGGRLLVDGHDVRDVTLASLRKQVGIVTQETVLFND
                     TVRNNIAYGQPHVAQREVEAAARAALAHDFIQALPAGYDTVIGERGVRLSGGERQRLA
                     IARALLKNAPILILDEATSALDSESEALVQSALHNLMVGRTVFVIAHRLSTVRRADRI
                     VVIENGAIADIGAHEELMQKLGTYRRLYELQFASVEPPKAVART"
     gene            2828..3748
                     /locus_tag="VEJ00_05000"
     CDS             2828..3748
                     /locus_tag="VEJ00_05000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXX00550.1"
                     /translation="MPIRSMTGYAQVKGEVQRTDHAGSADPHSRLAFVLSLKSVNHRF
                     LDLHFRLPSGTDSLEMQLRRLLKEKMARGHVEVSLSLERLADGSFSVNREIVGGYIAA
                     FRAVAAEFAVPADPDLNAILRIPGALGSASDCADGEIEGAVMAKVGEALERLNHMREE
                     EGWGIARELRERMAHLLQAGKSVQQYRRAVLQNYSERLQSRLQEMLGATVDKERVLQE
                     AALLVDRSDIQEEIVRLETHVQHFLGLLDEGGEVGKKLDFLLQEMNREANTLLSKTSG
                     LAGEALRITEAGLVMKAEIEKSREQVQNLE"
     gene            3745..4455
                     /gene="gmk"
                     /locus_tag="VEJ00_05005"
     CDS             3745..4455
                     /gene="gmk"
                     /locus_tag="VEJ00_05005"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXX00551.1"
                     /translation="MSETRQPIVYIISAPSGSGKSTLVNELLKLVPDLDFSISYTTRD
                     RRGSEQNGKEYNFVSREQFEEMIRADEFLEHATVFGKHYYGTAKRFLREAAAKGRDLL
                     LDIDVQGAAQIKQKLPDAVSIFVLPPDRKTLEWRLRKRSEDAEDVIQRRLVAASREIE
                     NYDKYDYVLINDKLEESVEGLQAIVRSVRLRRSGRALPGEEQRIVSLADGYRLANVRT
                     RVLPILASFGAPSPSARV"
     gene            4615..4848
                     /gene="rpoZ"
                     /locus_tag="VEJ00_05010"
     CDS             4615..4848
                     /gene="rpoZ"
                     /locus_tag="VEJ00_05010"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXX00552.1"
                     /translation="MKLIEGFDSNYRYILVAARRARQLQSGAPPVVDTNSRKPCRIAQ
                     DEIRAGKVKWEIPETRSAAEQAAETLDKGLGQE"
     gene            4941..6158
                     /gene="coaBC"
                     /locus_tag="VEJ00_05015"
     CDS             4941..6158
                     /gene="coaBC"
                     /locus_tag="VEJ00_05015"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HXX00553.1"
                     /translation="MKIALGVTGGIAAYKAAEIVRLLQDRGIRVQVVMTRAAQEFVRP
                     LTFAALSGEKVITSMFSSGEEQPANIDSAIEHIAVAQSIDALLVAPATADVIAHFAQG
                     IASDFLTTLYLATTAPVVVAPAMNVNMWNHPATQSNLQILRQRGVKIVEPGAGYLACG
                     MTGPGRLAENEAIVAAVLEALGASQDMAGETVLITAGPTREKIDPVRYLTNRSSGRMG
                     YALAEAALRRGARVLLVSGPTTLTPPGAAELTRVESAEEMRDAVLKLLPGASIVIKTA
                     AVSDYRPKAAADQKIKRKGPMTLEFEATPDILKELSARKSTQIVVGFAAETENVLENA
                     RQKLVAKNLDAIVVNDVSREGVGFDSDRNAVTIISREDVVEVPETTKWEVAQRVLDQV
                     VRLRQQRKSPVKA"
     gene            6201..7067
                     /locus_tag="VEJ00_05020"
     CDS             6201..7067
                     /locus_tag="VEJ00_05020"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXX00554.1"
                     /translation="MAYVLDPDVRRALAERVRYYNELGIYDFYQRTASMVEPETTVSS
                     SPQPEEREEMPVRKSAVVAAVAEENIFEVLTPKPEQGVADPASALKLIREDLGDCTRC
                     KLHKQGRKQIVFGVGNPRAELMFVGEGPGADEDAQGEPFVGRAGQLLNNMIKAMGLRR
                     EDVYIANIVKCRPPGNRTPERDECETCSPFLMRQIAVINPKVVVALGAVAAKNLLAIN
                     APMAELRGRFYDFMPAGVRRNDPAWPGAKLAVTYHPAFLLRDPRQKGEAWKDLQMVMK
                     YLGLKPQQKAES"
     gene            7178..>8340
                     /locus_tag="VEJ00_05025"
     CDS             7178..>8340
                     /locus_tag="VEJ00_05025"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXX00555.1"
                     /translation="MPEFSDVAVPVPLDMVFTYRVPSGSSPVVGGRVLVPFRQQRMTG
                     IVVELHDRTPSVTAKNIVGVVDSAPVLDEQLLRLGRWIADYYLAPLGEVFRTMLPLSA
                     EFRRSIGYRITEQGQMALHLAGMAGSSARSQRTPEEQAAEFRVLDYLAGHEAELGGAE
                     LVREQTLRAATQASRRILTGMVRKKWIVREDVSGPQVAVRIVKIAILRSAEGKLNDNQ
                     RVLVDTLAASGGRLSVEALRSLAVPRTTLGTLVRRGLVEVVEEPAEFTVSRTKPRASP
                     FDFDFNGAQQSALARLRDGVAARKFSGMLLHGVTGSGKTAVYLAAMRAVLEAGRSAIL
                     LVPEIGLTPAVAADLHQIFGDEVAILHSALSDRERAEQWHRIKAGEARVVVGTR"
BASE COUNT         1600 a         2347 c         2776 g         1617 t
ORIGIN      
        1 tttgccgagc tgtcgatgca gtcgagcgag agccgccgca cggcgctggt ggtggacgtg
       61 cgcgaggggg cgaagctcga gcagttgccg ggaattctaa agtcggtgcg gcgcatgatt
      121 ccgaccaagg tggtgttcct cgaggcctcg gatgcggtgc tgctgcggcg cttcagcgag
      181 acgcggaggc cgcatccgct gggcacggac gctccggtca aggctgcgct caaggcggag
      241 cggcggcatc tgggggcgat ccggcggctg gccgactatg tcatcgatac gtccaagttc
      301 aacgtgcatg agctgcgcgc gcacatcacc gagcgctttc aggagcaatc gtcggagaag
      361 agcattctgg tgtcgatcgt cagttttggt tttagggagg gcgtgccgga agatgccgac
      421 ctcgtcttcg atgtgcgctt cctgcccaac ccgcacttcg tgcccgagtt ccgtccgctg
      481 acgggacggg atccgcgggt ggcccgctat attcgatcgt ttccgcagac gcgcgaattt
      541 atcgcgcgca tctccgatct gctggtctac ctgctgccgc attacattca tgagggcaag
      601 gcgtacctga cgatcgcatt cggctgcacg ggaggacaac accgctccgt gatgatcgcc
      661 gaggaggtgg cgaagcacct gcggcgcgcg gactatcggg tgaaggtggt gcaccgggac
      721 agcccgaagt agggaatagg gacagaggta ggaggccctg gtatttccga tttgcgattg
      781 gcaattgggg atggcaaacg tcttcaagcc cgcccgcgaa gatcggagat ccccatgtgg
      841 tcgatcgaga ttcgttcgtc tggcagtcga gattaggatt cccgacccga ggtagaatac
      901 ggtcttgcat acgatacctc ttcgaccggc agccagcgct tgcggcctgc cccgcctggg
      961 agccccatgc ggcaactgat gcgccttgtc cgttacgttc ttccgtttct cctgcagtta
     1021 ttgccgggcg ttctcctgct ggccgcggtg ggatttctag aggcgttccg gttgatgctg
     1081 ctcaagccgg tactagaccg cgtgctgaat ccggcgtcgg gctcggagaa catcattctg
     1141 ttcacgtggc cggcgacggg cgagcctatc tacctgcagc gcttcgtgcc aacgcacttt
     1201 cacaacgcgt ggaccatcgt ggcgtacgcc ttggtggcat ctacggtgtt gaagggtgtg
     1261 ttcgactatg ccggcactta cctggtgaac catgccgggt tcggcatgat tacgaacctg
     1321 cgcaacgacc tgtacaactc ggtgctgcag cgctcggcgg cgttttttca gaagcacacg
     1381 acgggaactc tcatttcgac catcatcaac gacattgagc gtgtccagtt cgccatgtcg
     1441 agcattctgg ccgagtttct gcagcaggtg ttcacgttcg cgttcattgc ggtcgccgtg
     1501 gtgattctgg gccggcaatt ggcgtgggtg ctggtgctgt tcgttccgtt catcatctat
     1561 tcggcggtgc gaatcggccg gcgcgtgcgc acgaccacgc ggcgcgggca agaccaattg
     1621 gctgatgtgc agaacatttt gcacgagacc atcaccggca accgtatcgt caaggctttc
     1681 gggatggaat catgggaggt ggcgcgcttc cgcaaggcgg cgcagcggtt gttccgcgcc
     1741 aacctgcggt cggtggcggc ggctgcgatc agttcgccct tgatggacgt ctttggggca
     1801 atcgcgattg ccttgctgct gctgctgggt cgggaccgca tcgcgcacaa cgaaatgacc
     1861 ctgggcgcgt tcgtcacctt tgtggccgcg gtgttgagca tgtacaaccc ggtacgcaaa
     1921 tttgctgtgt ttaacaacag tttccagcag gcgctgggcg cgtcatcgga gttgttcaaa
     1981 ttcatcgacg cggaagatgt ggtgcgcgac aaagctggcg cgaagccgct gccgcggttt
     2041 tcagaaagca tccggtttga ggatgtgttc ttcaagtacg agggcgacgg cgacgaggcc
     2101 cgcgaagtgc tgcgcgggat cgacctcgaa gtcaagcggg gtgaggtgct ggcggtcgtg
     2161 ggctcgagcg gcgcggggaa aaccacgctg gtgcacctga tcccgaggtt tttcgacgtg
     2221 agcggaggtc gtctgctggt ggacggccac gacgtgcgcg atgtgacgct ggcgtcgttg
     2281 cgcaagcaag tcggcatcgt gacgcaggaa accgtgctct tcaacgacac ggtgcgcaac
     2341 aacatcgctt atggccagcc gcatgtcgcg caacgagagg tagaagcggc ggcgcgcgcg
     2401 gcgctggcgc acgacttcat tcaggcgctg ccggcgggat acgatactgt gatcggagag
     2461 cgcggggtgc ggctctccgg aggcgagcgg cagcggctgg cgattgctcg ggcgctgctg
     2521 aagaatgcgc ctatcctgat tctggacgaa gcgacgtccg cactggacag cgagtcggag
     2581 gccctggtgc aatcggcgct tcacaacctg atggttggcc gcaccgtgtt tgtgatcgcg
     2641 caccggctat ccaccgtgcg ccgcgccgat cgcattgtcg tgattgagaa cggcgccatc
     2701 gccgacatcg gcgcccacga ggaattgatg cagaagctcg gcacctaccg gcgtctctac
     2761 gagctgcaat tcgcgagtgt ggagccgccg aaggcagtag cgagaacctg agagtcggcc
     2821 acatcctatg cccatacgct ccatgacggg ttatgcgcag gtcaaaggtg aagtgcagcg
     2881 gacggatcac gctggatctg cggatcccca cagcagactg gcatttgtgc tgtcgctgaa
     2941 gtcggtgaac caccggttcc tcgatctgca tttccgcctg ccttcgggta cggactcgct
     3001 cgaaatgcag cttcggcggc tgctcaagga gaaaatggca cgcggtcatg tggaagtctc
     3061 gctgagcctg gagcgtctcg ccgacggcag cttctcggtg aaccgtgaga ttgttggggg
     3121 atacatcgcc gctttccgcg cggttgcggc cgaattcgcg gttccggcgg accctgacct
     3181 gaatgcgatt ctgcgtattc cgggcgcgct cgggtcggcg agcgactgtg ccgatggaga
     3241 gatcgagggc gccgtgatgg ccaaggtggg cgaagctctc gagcgattga accacatgcg
     3301 cgaggaagaa ggctggggca tcgcgcgcga actgcgcgag cgcatggcgc acctgctgca
     3361 ggcaggcaag agcgtgcagc agtatcgccg ggcggttctg cagaactaca gcgaacggct
     3421 gcagtcgcga ctgcaggaga tgctgggcgc tacggtggac aaggaacgtg tgctgcagga
     3481 ggctgcgctg ctggtcgatc gcagcgatat ccaggaagaa atcgtgcgcc tggagacgca
     3541 tgtgcagcat tttctgggat tgctcgacga aggtggcgag gtcgggaaga agctggactt
     3601 ccttctgcag gagatgaacc gcgaggccaa cactctactt tcgaagactt ccgggctggc
     3661 aggcgaggct cttaggatta ccgaagcggg tctggtgatg aaggcggaga tcgaaaaatc
     3721 gcgggagcag gtgcagaacc tggaatgagc gaaacgcgcc agccgatcgt ctacattatc
     3781 tccgcgccat cggggtcagg aaagtcgacc ctggtcaatg aacttctcaa actggttccc
     3841 gatctcgact tctccatctc ctataccacg cgtgatcgca gaggcagtga gcaaaacggc
     3901 aaggaataca atttcgtctc acgcgagcag tttgaggaaa tgattcgtgc cgacgagttc
     3961 ctggaacacg ctaccgtatt cgggaagcac tactacggca cggccaagcg tttcctgcgc
     4021 gaagccgccg cgaaggggcg agatctgctg ctcgatatcg acgtgcaggg cgctgcgcaa
     4081 atcaagcaaa agctgcccga cgcggtcagc atctttgtac tgccgcccga ccgcaaaacg
     4141 ctggagtggc gcctgcgcaa gcgcagcgaa gacgccgaag acgtcattca gcgccggctg
     4201 gtcgcggcca gccgcgagat cgagaactac gacaaatacg actacgtcct gatcaacgac
     4261 aagctggaag aatccgtcga gggcttgcag gccattgtgc gctcggtgcg cctgcggcgg
     4321 tcaggccggg cattgccggg cgaggagcag agaatcgtaa gcctggctga cggctaccgg
     4381 ttggcgaacg tccggacaag ggtgctgccc attctggcgt cctttggtgc tccctcgcct
     4441 tcggcacgcg tgtagttttc gcggtatcat ttcagggggc gtgaacgggg cgccgtagac
     4501 cggaaagaaa gtcataggcg ggacaagcct gatcgctggt gtgttctgcg gaacgcatag
     4561 ttcgacgttc ggcattgatt cacttcaaaa cctgttgttc aagggagagt cgcaatgaag
     4621 ttgatcgaag gcttcgacag caattaccgg tacattctgg tggcggcccg gcgggcgcgg
     4681 cagctgcagt ccggcgcgcc gccggtggtg gacacgaatt cccgcaagcc gtgccgcatc
     4741 gcacaggatg agatccgcgc gggcaaggtg aagtgggaga ttccggagac ccgcagcgct
     4801 gccgaacaag cggcggaaac cctcgacaag gggctggggc aggagtaggg cgaattttgt
     4861 gattgttgat tgttgaatgc ggccactgcc gcaggcaagc ggttttgaat caacaatcag
     4921 aaatcaacca tcaaaaattc atgaagatcg ctctgggcgt caccggcggg atcgcggcct
     4981 acaaggcggc cgagatcgtc cgactgctgc aggatcgcgg cattcgcgtg caggtggtca
     5041 tgacacgcgc cgcccaggag tttgtccggc ctcttacctt cgcggcgctt tccggcgaaa
     5101 aagttatcac cagcatgttc tcgtccggcg aggagcagcc ggccaatatc gactcggcga
     5161 tcgagcacat cgcggttgcg caatcgatcg atgcgctgct ggtcgccccg gctacagccg
     5221 acgttattgc gcacttcgca cagggcatcg ccagcgattt tctgacaacg ctctatctcg
     5281 ccaccaccgc tccggtcgtg gtggcgccgg cgatgaacgt caacatgtgg aatcatccag
     5341 cgacgcaatc caatctgcaa attcttcgtc agcggggcgt aaagatcgtc gagccgggcg
     5401 cgggttatct cgcgtgcggc atgaccggtc ccgggcgcct ggcggagaac gaggccatcg
     5461 tcgctgcggt gctggaagcg ctcggagcct cacaggatat ggccggcgag accgtgctga
     5521 ttacggctgg gccgacgcgg gaaaaaatag atccggtgcg gtatttaacg aaccgttcca
     5581 gcgggcgcat gggatatgcg ctggcggagg ctgcgttgcg ccggggagcg cgcgtgctac
     5641 tggtcagcgg gccaaccacg cttacgcctc cgggggcggc tgaactcaca cgcgtggagt
     5701 cggcggaaga gatgcgcgat gcggtgctca agcttctgcc cggggccagc atcgtgatca
     5761 agactgcggc cgtctccgac tatcggccca aggctgccgc tgatcagaag atcaagcgca
     5821 aaggcccgat gactctcgaa ttcgaggcga cgcctgacat cctgaaagag ctttccgcca
     5881 ggaagagcac gcagattgtg gtcgggtttg cggccgagac cgagaacgtc ctggagaacg
     5941 cgcgccagaa gctggttgcg aagaatctcg atgcaatcgt ggtcaacgat gtttcacgcg
     6001 agggcgtggg cttcgattcc gatcgcaatg cggtcaccat catttcccgc gaagatgtcg
     6061 tcgaagttcc cgagaccacc aaatgggaag tcgcccaacg cgtcctcgac caggttgtgc
     6121 ggctccgtca gcagcgcaaa tcgccggtga aagcctagtc cgacgacttc ggcaggttct
     6181 gcattacaat ctaaaaaccg atggcctacg ttcttgaccc ggatgtgcgg cgcgcgctcg
     6241 ccgagcgcgt gcgctattac aatgagctcg gcatctacga tttttaccag cgtacggcgt
     6301 cgatggttga acctgaaact accgtatcct caagtcccca gccagaagag cgagaagaaa
     6361 tgcctgtcag aaagtctgcg gttgtcgcgg ccgtcgctga agagaacatt ttcgaagtgc
     6421 tcacgcccaa gccggagcag ggcgtggccg atcctgcatc tgcgttgaag ctgatccgag
     6481 aagacctggg tgactgcaca cgctgcaaac tgcataagca gggacgcaag cagatcgtat
     6541 tcggagtagg gaatccgcgg gctgaattga tgttcgttgg cgaaggaccg ggagccgatg
     6601 aagatgccca aggcgagccg ttcgttggcc gagcggggca gttgctcaac aacatgatca
     6661 aggcgatggg cctgcggcgc gaggacgttt acatcgcgaa catcgttaaa tgccgtccgc
     6721 cgggcaatcg cacgccggag cgcgatgaat gcgaaacctg ctcgcccttt ttgatgcggc
     6781 agattgcggt gatcaatccg aaggtggtgg tggcgctggg cgcggtcgcg gccaagaatc
     6841 tgctggcgat caacgcgccc atggcggagc tgcgcgggcg cttctatgac ttcatgcccg
     6901 ccggcgtacg ccgtaacgac cccgcgtggc cgggagcaaa gctggccgtg acgtatcatc
     6961 ctgcatttct tttgcgcgat ccgcggcaga agggcgaggc gtggaaagat ttgcagatgg
     7021 tgatgaagta cctggggttg aagccacagc aaaaggctga atcgtaagtc tgccgcgcta
     7081 gcggggcgga gtatccgcaa gagatgatct gcggagtcga aacgtccctg cgcaaccttc
     7141 cgcgcaggca cagccttctg atttattctt ccacacaatg cctgaattct ccgatgtcgc
     7201 cgtgcccgtg ccgctggaca tggtattcac gtaccgcgtg ccttcgggta gctctccggt
     7261 ggtcggcggg cgggtgttgg ttccctttcg acagcaaagg atgacgggca ttgttgtcga
     7321 gttgcacgac cgtacgccgt ccgttacggc aaagaacatc gtcggagtgg tcgactccgc
     7381 tccggtgctc gacgaacaac tgttgcggct cgggcgctgg atcgccgatt actacctggc
     7441 tccattgggt gaagtcttcc gcaccatgct gccgttaagc gccgagttca gacgctccat
     7501 cggctatcgg atcacggagc aagggcagat ggcgctgcat ctggcgggca tggcgggatc
     7561 gtccgcgcga tcgcagcgta cgcccgagga acaggccgcg gagtttcgcg tactcgacta
     7621 tctggctggg cacgaggctg agctcggagg cgcggaactt gtgagggagc agacattgcg
     7681 tgcggccact caggcatcga ggcggatcct cacgggaatg gttcggaaaa aatggatagt
     7741 gcgtgaggac gtctcagggc cgcaagtcgc ggtgcgaatc gtaaagattg cgattctgag
     7801 atctgccgag ggcaaactca atgacaacca gcgcgtgctg gtcgacacgc tggctgcgtc
     7861 cgggggcaga ctctccgtgg aagcgctacg ctcgctggcg gtgccgcgca cgacgctagg
     7921 cacactggtg cggcgtgggc tggtcgaggt cgtcgaggaa cctgcggaat ttacggtctc
     7981 gcgtacgaaa ccgcgggcgt cgccgtttga ctttgatttc aacggtgcgc agcagtcggc
     8041 gctggcgcgt ctgcgcgatg gcgtcgcggc gcgaaaattt tccggcatgc ttctgcatgg
     8101 cgtgaccggg tcggggaaga ctgccgtcta tctggccgca atgcgggcgg tgctcgaagc
     8161 tggacggtcg gcaatcctgc tcgttccgga gatcgggctc acgccggccg tcgccgccga
     8221 cctgcatcag atctttggcg atgaagtcgc cattttgcac tctgctctct cggaccgcga
     8281 gcgcgccgag cagtggcatc gcatcaaggc cggggaagcg cgggtggtgg tgggcacgcg
//
LOCUS       DASPUL010000212         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_586318, whole genome shotgun sequence.
ACCESSION   DASPUL010000212 DASPUL010000000
VERSION     DASPUL010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586318"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..301
                     /locus_tag="VEJ00_05030"
     CDS             80..301
                     /locus_tag="VEJ00_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00556.1"
                     /translation="MNDFNRKVEDASARVGKSISDAAERLEKEIPEFIKYLNEEVVPA
                     VRTHSTKALRVASQKLAEFADYMEQHPQK"
     gene            364..1137
                     /locus_tag="VEJ00_05035"
     CDS             364..1137
                     /locus_tag="VEJ00_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXX00557.1"
                     /translation="MLLILLLTLLLSACSHPKQARVEVPPPPPPASTSTTAELSPPPA
                     ANIPARSTTRVPAEPRIDPDLAEPILPADTKPLVTETGLASWYGPPYHNRRGSNGEVY
                     NMHAMTAAHRTFPLGSIVRVTNLKTGHAALVRITDRGPFIPGRVVDLSLAAARKVDVV
                     QPGVAEVKVELMQTGAPLDSAGKWAVQIGGFPDEKSASKLADHLTHRYHTAKVLRFAS
                     PAGDWWIRVRVLDDDHERAQQLAAETSTPEGAVFLVRLD"
     gene            complement(1476..2525)
                     /locus_tag="VEJ00_05040"
     CDS             complement(1476..2525)
                     /locus_tag="VEJ00_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXX00558.1"
                     /translation="MSSLLEVRHLNIEFPALSSPAAAPRAAGQPGAAVPTNSLFAVRD
                     LSFSIAPGEVLGLVGESGSGKSLTSLAIMRLLPPQARASGEILLAEDRSGLCNLTERS
                     DEAMRRLRGSRIAMIFQEPMTALNPVMRVGDQIAEAVLAHHAVSKKEAWQRAVDAMNN
                     VAIPDAPLRARDYPHQLSGGMRQRIMIAMAIVNRPQLLIADEPTTALDVTIQAQILEL
                     LGELRARFGLAMLFISHDLAVVSQVADRVAVMYAGSVIEVGTKRDIFQAPAHPYTRGL
                     LHAVPDLKTDRARPLETIEGTVPALQAMPAGCAFEPRCGFRIAECARALPPLVEVAAG
                     HWARCPVVNSQSVAG"
     gene            complement(2611..>3061)
                     /locus_tag="VEJ00_05045"
     CDS             complement(2611..>3061)
                     /locus_tag="VEJ00_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXX00559.1"
                     /translation="PTLRSAIRESTASHTANALYTGEREMVAKQIFDQVSAQLSQRGI
                     TVENVLLRDIQLPATLKASIEAKQQAEQEALAMNFRLQKETQEAQRKRIEAAGVRDFQ
                     QIVAQGITPSLLEWKGIEATENLAKSANTKVVVIGNNKNGLPLILGQ"
BASE COUNT          585 a         1034 c          898 g          544 t
ORIGIN      
        1 cgccacgaaa taatagtggg gcctggcatc cgcaacatcc gcgtgagtcg attcacgccc
       61 gttcgtcgcg aggagcacca tgaacgactt caaccgcaag gtggaagacg cgtccgcgcg
      121 tgtcggcaag agcatcagcg acgccgccga gcgtctggaa aaggaaattc ccgaattcat
      181 caagtacctg aacgaagaag ttgtcccggc cgtgcgcacg cactccacca aggcgctgcg
      241 cgtggcttcg cagaagctcg ccgaattcgc cgactacatg gagcagcatc ctcagaagta
      301 gaaagcagca tctcacttgg gccccgccgt acccgtagtc gcagacactt cgccagcgtc
      361 gtcatgttgc tgatcctgct gcttacgttg ttgctctcgg cctgcagtca tcccaagcag
      421 gcgcgcgtgg aggttccgcc gcccccgccg cccgcgtcaa catcgacgac cgcagaactg
      481 tctccaccgc ccgccgcgaa tattccggcg cggtcaacaa ctcgtgtgcc cgcggaaccc
      541 aggattgacc ccgatctcgc cgagcctatt ctccccgccg acaccaagcc cctcgtcacc
      601 gaaaccgggc tggccagctg gtacggccct ccctatcaca atcgccgcgg atcgaacggc
      661 gaggtctaca acatgcacgc catgaccgcc gcgcaccgca cctttccgct ggggtcgatc
      721 gtgcgtgtga ccaatctcaa gaccggccac gcggcgctgg tgcgcatcac cgaccgcggg
      781 ccgttcattc ccggacgcgt ggtcgatctc tcgctcgccg ccgcgcgcaa ggtggacgtc
      841 gtgcagcccg gcgtcgccga agtcaaagtt gagttgatgc agactggcgc gccgcttgac
      901 tcggccggaa agtgggctgt gcagatcggc ggcttccccg acgagaagtc tgcttccaag
      961 ctcgccgatc acctcaccca ccgctaccac acggccaagg tccttcgttt tgccagtccc
     1021 gccggggatt ggtggatccg cgtccgcgtg ctcgacgatg accacgagcg cgcccagcaa
     1081 ctcgccgctg aaacctcgac tcctgagggc gcggtcttcc tggtgcggct ggactagcga
     1141 ttcgcgagcg cgaagttgtg cgtcgaaatc cagcctgtga agctggcatt gcgcttttct
     1201 cggcgtcctt tgcggccgtt ccttcgcgaa ccttgcggta aaaggactaa tcgcaaacat
     1261 cgcgaagagg atccgcaaag agcgcaaaga agagccgctg gggcataacc tggcggagat
     1321 tccgcgaaca ccgaggaaag cacagggctc caaccgagtt gccagaagcc aggaatcccg
     1381 tggaagagcg gccctttagg gtcgcgtaaa gggcaagaaa ccgacgccct tcagggcggg
     1441 tggtcgcgtt cgtcactatt gccagggacg cgtgcttagc cagcgacgga ttgagaattc
     1501 actaccgggc atcgcgccca atgtcccgcc gccacttcga ccaacggcgg caacgcccgc
     1561 gcgcactccg caatacggaa tccgcatcgc ggctcgaacg cgcatcccgc aggcatcgcc
     1621 tgcagcgctg gaacggttcc ttcaatcgtt tccagcggcc gcgcccgatc cgtcttgaga
     1681 tccggcacgg cgtgcagcaa tccccgcgta tacggatgcg ccggcgcctg aaaaatgtcc
     1741 cgcttcgtgc ccacttcgat cacgctgccc gcgtacatca ccgccacgcg atcggctacc
     1801 tgcgacacga ccgccaaatc gtgggagata aaaagcatgg ccagcccaaa ccgtgcccgc
     1861 aattcgccga gcaactccag aatctgcgcc tgaatggtca cgtcgagcgc cgtcgtcggc
     1921 tcatcggcaa tcagcagctg cggacggttc acgatggcca tcgcaatcat gatgcgctgc
     1981 cgcatccctc ccgaaagctg atgcggatag tcgcgcgccc gcagcggcgc atccggaatc
     2041 gccacgttat tcatcgcatc gacggcccgc tgccaggctt cttttttcga aaccgcatga
     2101 tgcgccagca cggcctcggc gatctgatcg cccacgcgca tcaccggatt cagcgcggtc
     2161 atgggctctt gaaaaatcat ggcgatgcgc gatccgcgca ggcggcgcat ggcctcatcg
     2221 gagcgttcgg tcagattgca caggccggat ctatcttctg ccagaagaat ctcccccgaa
     2281 gcccgcgcct ggggcggcaa cagccgcatg atggcaagag atgtaagcga cttgcccgaa
     2341 cccgattccc ccaccaaccc cagcacctcg cctggcgcaa tggaaaagct cagatcgcga
     2401 acggcgaaaa gagaatttgt agggacagcc gcccccggct gtccggcggc ccgcggggcc
     2461 gcggctggcg aagacagcgc cggaaactcg atgttcaaat gtcgaacttc gagcagcgaa
     2521 ctcacgctcg ctatggtagc agcggccagc gccgcccggc gggcggcgga cagccgaggg
     2581 cggctgtctc tacatggcta cttccccgat ctactgaccc agaatcagcg gcagcccgtt
     2641 cttgttgttg ccgatcacca ccactttggt gttggcgctt ttggccaggt tctccgtggc
     2701 ctcgattcct ttccattcca gcagcgaggg cgtgatgccc tgggcgacga tctgttggaa
     2761 atcgcgcacg ccggctgcct cgatgcgctt gcgctgcgct tcctgcgttt ccttctgcag
     2821 acggaagttc atggccagcg cttcctgttc ggcctgctgc ttggcttcga tggaagcttt
     2881 cagcgtggcg ggcagctgga tatcgcgcaa cagaacgttc tcgaccgtga tgccacgctg
     2941 gctcagctgc gcgctcacct ggtcaaaaat ctgcttggct accatttcgc gttcgccggt
     3001 gtagagcgca ttcgccgtgt gcgaggccgt ggattcccga atcgcggagc gcaaggtcgg
     3061 c
//
LOCUS       DASPUL010000213         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1952864, whole genome shotgun sequence.
ACCESSION   DASPUL010000213 DASPUL010000000
VERSION     DASPUL010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1952864"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VEJ00_05050"
     CDS             complement(<1..245)
                     /locus_tag="VEJ00_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00560.1"
                     /translation="MELDSTLSSVLIDRYSANAARVRELAAPLANAEFWRKPYPYGNS
                     FGHLVLHLSGNLNYYIGAQIAKTGYDRDRPREFNDANP"
     gene            complement(283..1362)
                     /gene="prfA"
                     /locus_tag="VEJ00_05055"
     CDS             complement(283..1362)
                     /gene="prfA"
                     /locus_tag="VEJ00_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXX00561.1"
                     /translation="MFERLDQIEARYEELTQALASPEIINDSARYQKTAKAHAEIAPV
                     VEKYREYKDLTKGIAESKAVLGDEKDPDMRAYAQEELDKLQARVASVEEELKVLLLPK
                     DPNDEKNVILEIRAGTGGDEATLFAAEVFRMYTRYAETQRWKVEVLSASESSVGGLKE
                     VIAIIEGNRVYSQLKYESGVHRVQRVPATEQQGRVHTSAITVAVLPEAEEVDIKIEPK
                     DLRIDTFCSSGPGGQSVNTTYSAVRITHIPTNTVVSCQDEKSQIKNREKGMRVLRARL
                     YEIEMEKQQEALAKERRAMVKSGDRSEKIRTYNFPQNRVTDHRIGFTLHQLDVVMDGK
                     IQPLIEALVTYYQAEKLKQETAGVV"
     gene            complement(1504..2601)
                     /locus_tag="VEJ00_05060"
     CDS             complement(1504..2601)
                     /locus_tag="VEJ00_05060"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HXX00562.1"
                     /translation="MRFLWNYLRFLAAVQLLPALESGEETLVGGQAVLEGVMMRSPHA
                     WAIACRKPSGEVVTMSEPLERPSERHKWMAWPLLRGVMTLGYAMNLGYRALRFSASVA
                     IEDVMQAENAEVATLGVVPRHSVRQGQITLPPTAVVAPPSAKAETRAKAAKLSNWLAI
                     GNLLLSLAFFIFMYKYIPLLAATELKRIDPALGGRIIFNLIDGGVRLGLFLLFIWGVS
                     LWKDIRRVYEYHGAEHKTVFAFENGDPLEVSAVQKYSTYHPRCGTSFLMTVMLISIGF
                     YMLIPFTTFWARFASRIVLLPIIAGVSYEIIRFAAKHRGSLFALMTAPGLWLQRITTK
                     PPSDDQAQCAITALDQAMALEKEHGGELVIA"
     gene            2791..>3492
                     /locus_tag="VEJ00_05065"
     CDS             2791..>3492
                     /locus_tag="VEJ00_05065"
                     /inference="COORDINATES: protein motif:HMM:NF016553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00563.1"
                     /translation="MQRLATACEAIAATTKKLQKTSIVAEYFRSRTVEEAAVSAVFLS
                     GRAFPAWAETTLQIGGRSIWQIVAELAGKGESDLTAAYRRLGDLGAVARDVLPQRPGQ
                     GIGLLEVSEGFRQIAAARGTAAKTALVCELLSRATPLEAKYIVKIMTGDLRIGLKESL
                     VEEAVARAYGAPAGELKDIQRANMLLGDIGETLRMAAGGKLADARLRMFHPLGFMLAS
                     PIDSAEEGLSYFSEAA"
BASE COUNT          712 a          981 c         1064 g          735 t
ORIGIN      
        1 ggattggcgt cgttaaattc gcgcggccga tcgcgatcat agccagtctt ggcgatctgt
       61 gcgccaatgt aatagttcag attgccggac aagtgcagca ccaagtggcc aaagctgttt
      121 ccgtagggat aaggctttcg ccagaattcc gcattagcca acggcgcggc cagctcgcgc
      181 acacgggcag cgttcgcgga gtaacggtcg ataaggacgg aggaaagtgt ggagtccagt
      241 tccatgtcgt caggttagca ttttttctta acctgacctt cgtcacacga ctcccgccgt
      301 ttcctgcttc agcttctctg cctggtagta agtcacgaga gcttcgatga gaggctgaat
      361 cttgccatcc ataacaacat cgagctggtg cagggtgaaa ccgatgcggt gatcggtgac
      421 gcggttttgg ggaaagttgt acgttcggat cttctcgctg cgatccccag atttgaccat
      481 ggcgcgccgt tctttcgcga gcgcctcttg ctgcttctcc atctcgattt cgtagagccg
      541 ggctcgcagt acacgcatgc ccttctcgcg attcttgatc tgcgactttt cgtcctggca
      601 gctcacgact gtgtttgtag gaatgtgggt aatgcgcaca gccgagtagg tcgtgttgac
      661 cgattggccg ccggggccgg aggagcagaa tgtgtcgatg cgcaagtctt taggttcgat
      721 cttgatgtcg acctcctctg cttccggcag cactgcaacg gtaatcgccg acgtgtgcac
      781 ccggccctgc tgttcggtcg ccggtacacg ctgcacccgg tgtacgccgc tctcgtattt
      841 cagctgtgaa tagacccgat tcccttcaat gatggcgatg acctctttca atccgccgac
      901 cgaagactcc gacgccgaaa gcacctcgac cttccagcgc tgcgtttccg cgtaccgggt
      961 atacatgcgg aagacctcgg ctgcaaaaag ggtggcttcg tctccaccag tgcccgcacg
     1021 aatctcgagg atgacgttct tttcatcatt cgggtccttg ggcaagagca gcaccttcaa
     1081 ctcttcctcg acggaagcga cccgagcctg gagtttgtcg agttcttcct gcgcataggc
     1141 acgcatgtcg gggtctttct cgtcgccgag aacagccttg ctctcggcga tgcccttggt
     1201 cagatctttg tactcgcggt acttctcgac gaccggagcg atttcggcgt gcgctttggc
     1261 tgtcttctga taccgcgccg aatcattgat gatctccggc gacgcaagag cctgggtgag
     1321 ctcttcgtag cgggcttcga tttggtctaa gcgttcaaac atgtcagatg gctttcctcg
     1381 acgtagcttc ctacatctca gtgagccgtg gacagtcctg gccggaatcc ggactcgtcg
     1441 atgacgtcct acggcattgt agtgatcttg ggagacggaa aggaagtatg cgcggcggag
     1501 ccgctaggcg atgactaatt ccccgccatg ctccttttca agcgccatgg cttgatcaag
     1561 ggcagtaatg gcgcattgcg cctgatcgtc agagggaggc ttcgtcgtga tgcgctgcag
     1621 ccacagaccg ggcgctgtca tcagcgcaaa gagcgatccc cgatgcttcg cggcgaaccg
     1681 gatgatctcg taggaaactc cggcgataat cggcagcaaa acgatacggg aggcgaaacg
     1741 ggcccagaag gtcgtgaatg gaatcagcat gtaaaagccg atggaaatta acatgaccgt
     1801 catcaggaaa ctggtcccgc agcgcggatg gtacgtcgaa tatttttgca cagccgaaac
     1861 ttccagtgga tcgccgttct caaaagcaaa aaccgtcttg tgctcggctc cgtgatattc
     1921 atagacgcgg cgaatgtcct tccacagtga aactccccag atgaagagca ggaaaagacc
     1981 gagccgaact cccccgtcga tcagattgaa aatgatccgg ccgcccagcg cgggatcgat
     2041 ccttttgagc tccgtcgcgg ccagcagggg aatgtatttg tacatgaaga tgaagaaagc
     2101 cagcgaaagc agcaggttgc caatcgccaa ccagttgctg agtttggctg ccttggcccg
     2161 agtctcagct ttcgcagaag gaggcgcaac cacggcggtt ggcggcagcg taatctgccc
     2221 ctggcgcacc gagtgccttg ggactacccc cagcgtcgcc acttcggcat tctcggcctg
     2281 catcacgtct tcgatggcaa cgctggctga gaagcgtaag gcgcggtacc cgaggttcat
     2341 ggcgtatccc agcgtcatta cgccccgcag gaggggccag gccatccatt tgtgcctttc
     2401 ggaggggcgc tcgagcggtt cgctcatggt aaccacttcg ccagagggct tgcggcaggc
     2461 gatggcccac gcgtgcgggg accgcatcat cacgccctca agcacagcct gaccgccaac
     2521 taaggtctct tcgccgctct cgagagcggg caggagttgc acggccgcaa gaaaacgcag
     2581 gtagttccac agaaaacgca caggttttag atgatccttc cgggggaagc gcaccaatgt
     2641 agtaggttat cacaggtcca gaaccggttc cgagattggc gggatccatt cgaactatat
     2701 gcattcgtga gacttaggcc ttgtcgaggt tgaagtttgc gagcgtcgat cgggcgtgtt
     2761 ggcccgggga ggcgccccac cagggaatct atgcaacgac tggcgacagc ttgtgaggcg
     2821 atcgcggcca cgacgaagaa actgcaaaag acatcgatcg tggccgaata tttcaggtcg
     2881 cgcacggtcg aggaggcggc tgtgtcggcg gtctttctct ccggacgagc gtttcccgcc
     2941 tgggcggaaa cgactctcca gatcggcggc aggtcgattt ggcagattgt ggcggagctg
     3001 gcaggaaagg gcgaaagtga cctgactgcc gcgtaccgac gactcggcga cttgggagcg
     3061 gtagcgcgcg atgttcttcc gcagcggccg ggccagggga ttggcctcct ggaggtaagc
     3121 gaaggttttc ggcagatcgc ggcggcccgc ggaacggcgg cgaagactgc actggtgtgc
     3181 gaactgctgt cccgggcgac cccgctcgaa gccaagtaca tcgtcaagat catgaccggc
     3241 gatctgcgaa tcgggctcaa agagagcctg gtcgaagagg cagtcgcaag agcatatggc
     3301 gcaccggcgg gtgaactgaa agacattcag cgcgccaaca tgttgctggg agacatcggc
     3361 gagactttgc ggatggcggc gggcggcaag ctggccgacg ccaggctgcg gatgtttcac
     3421 cctctcggat tcatgctggc cagcccgatt gactcggcgg aggaaggact gagttacttc
     3481 tccgaagcgg cc
//
LOCUS       DASPUL010000214         4339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1814565, whole genome shotgun sequence.
ACCESSION   DASPUL010000214 DASPUL010000000
VERSION     DASPUL010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814565"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..827
                     /locus_tag="VEJ00_05070"
     CDS             33..827
                     /locus_tag="VEJ00_05070"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXX00564.1"
                     /translation="MNKRTGWAAFSVSMLALCALLILAACNKKEEAPASNTMAEQPAS
                     APAATPIDPGTVATVSGTVKFDGTAPKAAKIDMSQDPGCKGTNEAENVAVSNGDLANV
                     FVYVKDGLGNRTFDVPKDAVVLDQQGCRYHPHVLGVMTGQTVQIKNDDPTTHNIHPTP
                     KDNREWNESQPPSSPALEKNFAREEVMLPVKCNQHPWMKMYINVVKSPFYAVTDKDGK
                     YEIRGLPPGDYTLAFVQEKLGEQDQKVTLAAKESKTVDQSFKPAAD"
     gene            927..1895
                     /locus_tag="VEJ00_05075"
     CDS             927..1895
                     /locus_tag="VEJ00_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HXX00565.1"
                     /translation="MHLPTQRNTPLKGIESPYHHGHHAFAVFTACATLVVITAGALVT
                     SNDAGLSVPDWPTSFGYLIKVPHFVGGIRYEWTHRMLAGSLLTLTLGIALWTVLVERR
                     RWMRWLAVGAFSTVIAQAILGGLTVLLLQPPAVSAAHATVAQTFFCIAVVIAVFSGRR
                     WVEEQPRLEFDQRHPSLFTLTLLSIFVLYVQLILGAMFRHHGMSWWPHLANAVIVSFV
                     LSWTAVRTLSVYSQIEAVRRPAVLMLSLMIAQLCLGFAAFLTRVEWGKDAVQPELPMV
                     MSTVAHVAVGALLLATAVVLAIQVWRHVPVAFEERLPQVQRDPSAA"
     gene            2047..3081
                     /locus_tag="VEJ00_05080"
     CDS             2047..3081
                     /locus_tag="VEJ00_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXX00566.1"
                     /translation="MVSKVSAFADADPEVEGSSAPPVISVQNLVHRYESRAALNGVSF
                     DVLPAELFGLLGPNGSGKTTLFRILSTLMIPTGGRAVVMGCDAAQDAASLRRQIGVVF
                     QAQSVDPKLTAYENLWHQGHLYGLRGTALKKRIGEILTRVGLADRSGERVETFSGGMQ
                     RRIELAKGLLHHPGVLLLDEPTTGLDPGARRDLWQYLQMLRDEEHVSVLVTTHLMEEA
                     ERCDRLAIMNEGNLVALGTPAELKSEIGGDVILLDAAGDAAVLAEHIRQRFDVDATAL
                     GKQVRIEREGGHRFVPDLVEAFPGEIQAISVSKPALEDVFIHRTGHKFWSEESEPDSS
                     ESKKAKKKRR"
     gene            3083..3340
                     /locus_tag="VEJ00_05085"
     CDS             3083..3340
                     /locus_tag="VEJ00_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00567.1"
                     /translation="MRSMILALSLILGVLPLAGIGWIFASGMITLWPFSATVDGLFMT
                     LILLTLSACFLLNAYWEMRDQGMIGKKKDTAPAKAPAPKAG"
     gene            3345..4172
                     /locus_tag="VEJ00_05090"
     CDS             3345..4172
                     /locus_tag="VEJ00_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX00568.1"
                     /translation="MATQATTLARPVAQASAGVLLPAFTLWWREIVRFYRQTTRVIGV
                     LASPLVFWLVIGSGFGTSFRSGGGPGQQHYLDYFYPGALIMIVLFTSIFTMMSVIEDR
                     KQGFLLSVLVAPVPRTAIVLGKVLGGTTLSAIQGMIFLIFAPFAGVHLDPLQVLLVAV
                     VVFLVSFALTALGFAIAWPMDSSQAFHGIVNLFLIPLWLVSGALFPMTGASGWIRAIM
                     RLNPLTYGVEALRGLLYPGMETQFPLSSAMATLVLFSLVMFGLAFLMANRRSTRPAA"
BASE COUNT          773 a         1265 c         1346 g          955 t
ORIGIN      
        1 gtcaactgaa cttctaacga aagggcgaca tcatgaataa gagaacgggt tgggcagcat
       61 tctcagtaag catgctggcc ttgtgtgctt tactcattct ggcggcctgc aacaagaagg
      121 aagaagctcc ggcctccaac acaatggcgg agcagcccgc gtcggcgccc gcggcaactc
      181 cgattgatcc agggaccgta gccacagtca gcggtacggt caagttcgat ggtacagccc
      241 cgaaagcagc caagatcgac atgagccagg atcccggctg caaagggacc aacgaagccg
      301 agaatgtcgc cgtgtcgaac ggcgacctgg ccaacgtttt cgtctacgtg aaagacggcc
      361 tgggcaatcg cactttcgac gtgcccaagg atgccgtcgt gctcgatcag cagggctgcc
      421 gctatcatcc ccatgtgttg ggcgtgatga cgggacagac cgtgcagatc aagaacgacg
      481 atccgaccac gcacaatatc catcccaccc cgaaggataa tcgcgagtgg aacgaatcgc
      541 agccgccgtc ttcgcctgca ctcgagaaga actttgctcg cgaggaagtc atgctgccgg
      601 tgaagtgtaa ccagcacccc tggatgaaga tgtacatcaa cgtggtgaag agcccgttct
      661 atgccgtcac tgacaaggac ggcaagtacg agatcagggg ccttccgccg ggagactaca
      721 cgctggcctt cgtgcaggag aaactcggcg agcaggacca gaaggtcacg ctcgcggcga
      781 aagagtcgaa gaccgtcgac cagagcttca aaccggctgc cgactagcgc tccgtctggt
      841 aacataaggg gttggacagg gctggctgca ccggctgtgc ggcttgctct gttctcctgt
      901 ttgcagcgcc ggagcgaact gcctctgtgc acctcccgac tcaaaggaac acacctctga
      961 agggcattga gtcgccctat caccacgggc accatgcctt cgctgtcttc accgcctgcg
     1021 ccacgctggt cgtgatcact gcgggcgcac tggtcaccag taacgatgcc ggcctgtcgg
     1081 tgccggactg gcctacgtcg tttggatatc tcattaaagt tcctcatttt gtcggtggca
     1141 ttcgctatga gtggacgcac cgcatgctgg cgggatccct tctgacgctc accctgggca
     1201 tcgctttgtg gacggtgttg gtcgagcggc ggcgctggat gcgctggctg gcggtggggg
     1261 ccttttctac ggtgattgct caggcgatcc tgggagggct caccgtgctg ttgttgcagc
     1321 cgccagcggt ttccgcggct catgccacgg tcgcccagac ctttttctgc attgccgttg
     1381 taatcgcggt gttcagcggg cgcagatggg tagaggaaca accgcgcctc gagttcgatc
     1441 agcggcatcc cagcctcttc acgctgacgt tgctgtccat ctttgtgctg tatgtgcagt
     1501 tgattctggg agccatgttc cgccatcacg gcatgagctg gtggccgcat ctggccaacg
     1561 ccgtaatcgt ttcgttcgtc ctgtcatgga cggcagtgcg gacactgagt gtctactcgc
     1621 agatcgaggc ggtgcgccgc cccgccgttc tgatgctgtc gctgatgatc gctcagctct
     1681 gccttggctt cgctgccttc ctgacgcggg tcgagtgggg gaaggacgcg gtgcaaccgg
     1741 agttgccgat ggtcatgtca accgtcgccc acgttgcggt gggagcgttg ttgctagcaa
     1801 cggccgtggt tctggcgatc caggtgtggc gccatgttcc cgtcgccttc gaggaacggc
     1861 ttccgcaagt gcagcgggat ccttcggctg cgtgagtacc gtccttgtgc aaccgacatt
     1921 gatggcggat tgctcgcttc agacccgcaa cttctcgcgg cggggggtgt tctacttgcc
     1981 aagcccgcct ctggcgcgga tagccgatcg tatgtcgtaa actggttcta gtcctgggga
     2041 tgggttttgg tctcaaaagt aagcgcattc gccgatgccg atccggaggt ggaaggttct
     2101 tccgccccgc ctgtcatttc cgtacagaat ctggtccatc gttatgagag tcgcgcggcg
     2161 ctgaacggag tatcgttcga cgtcctgccg gccgagttgt tcgggttgct tggcccgaat
     2221 ggaagcggga aaacgactct cttccgaatt ctctccaccc tgatgattcc gaccgggggc
     2281 cgcgctgtcg ttatgggatg cgatgcggcg caggacgcgg ccagcctgcg gcggcagatt
     2341 ggggtcgttt ttcaggcgca aagcgtcgat cccaagctca ctgcgtacga aaacctatgg
     2401 caccagggac atctttacgg actgcgggga acggcgctga agaagcgaat cggggagatt
     2461 ttgacgcgcg ttggcctggc tgatcgatcg ggcgagcggg ttgagacatt ctctggcggc
     2521 atgcaacgcc gcattgaact ggccaagggg cttctgcacc atcccggtgt tttgctgctc
     2581 gacgaaccga cgaccggtct cgatcccggc gcccgacgcg acctgtggca gtacttgcag
     2641 atgctgcggg atgaagaaca cgtctcggtt ctggtaacga cccatttgat ggaagaagcc
     2701 gagcgctgcg accggctggc catcatgaac gagggcaacc tggtggcgct gggaacgccc
     2761 gcggaattga agagcgagat cggcggcgac gtgattttgc tcgacgccgc gggcgacgcc
     2821 gcggtgctag ccgaacatat ccgccagcgc tttgacgtgg atgccactgc tctgggcaaa
     2881 caggtccgga ttgagcgcga aggcggccac cgcttcgttc ccgacctggt cgaggccttt
     2941 cccggcgaga tacaggcgat ttcggtgagc aagccggcgc tggaagatgt gttcatccac
     3001 cgtacgggac acaagttctg gagtgaagag agcgaaccgg attcttccga atccaaaaaa
     3061 gctaagaaga aaaggaggta gcttgcgttc gatgatcctt gcgctttccc tcatcctggg
     3121 agtgctgccc ctggctggaa ttgggtggat ttttgccagc gggatgatca ccctctggcc
     3181 gttctcagca actgtcgacg gcctgttcat gaccctcatt ttgctcactt tgtcggcctg
     3241 ctttctgctg aacgcgtatt gggagatgcg cgaccagggc atgatcggca agaagaaaga
     3301 cactgcgccg gcaaaggctc ccgcgccgaa ggcaggttaa gcccatggcg actcaggcaa
     3361 caacgttggc gcgacccgtc gctcaagcgt cggcgggcgt gctactgccg gctttcacct
     3421 tgtggtggcg ggaaattgtg cgcttttacc ggcaaaccac acgggtgatt ggtgtacttg
     3481 cgtcgccgct ggtgttctgg ctcgtcatcg gttcggggtt cggtacttcg tttcgctccg
     3541 gtggcggacc tggccagcag cactatctgg actactttta tcccggcgcg ctgatcatga
     3601 tcgtgctctt cacctcgatt ttcaccatga tgtcggtaat cgaagaccgc aagcaaggat
     3661 ttctgctctc ggtgctggtt gcgcccgtgc cgcgaaccgc gattgtgctg ggcaaggttc
     3721 tcggcgggac gacgctctcg gctattcagg gaatgatctt tctgatcttc gcccccttcg
     3781 ccggagtgca tcttgatccg ctgcaggtct tgctggtagc ggtggtggtc tttctggtct
     3841 ccttcgccct gaccgcgctg ggcttcgcca tcgcctggcc catggactcg tcgcaggcct
     3901 ttcacggcat cgtcaatctc tttctgattc ctctttggct ggtgtcggga gcgctatttc
     3961 ccatgaccgg cgcctctgga tggattagag ccatcatgcg actgaatccg ctaacctacg
     4021 gcgtggaagc gctgcgcggg ttgctgtatc cgggcatgga aacgcagttt ccgctctctt
     4081 ccgccatggc cacgttggtc ctgttttcgc tcgtcatgtt cggtctcgcg ttcctgatgg
     4141 ccaaccgccg cagcacgagg cccgccgctt gattcccgaa cagtacgcct tcttcccggc
     4201 cctgaacgcg ggcctgaacg gaaccagcgc cgtcctgctg ctgagcgggc gtgttctcat
     4261 tgcgaagaaa cgtatcgccg cgcaccgcgc ctgcatgatt gcggcggtgg ttgcgtcggc
     4321 tctgtttctg ggatgctat
//
LOCUS       DASPUL010000215         4460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_312638, whole genome shotgun sequence.
ACCESSION   DASPUL010000215 DASPUL010000000
VERSION     DASPUL010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312638"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..3185
                     /locus_tag="VEJ00_05095"
     CDS             3..3185
                     /locus_tag="VEJ00_05095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HXX00569.1"
                     /translation="MYGAPRFHLAAQKAKIKAHIGAEITCTPLNSVILSEDALARSAR
                     ASQSKNPYSQQSMRKVGIPRSVRNDKPFRLPLLVASRTGYQNLCSLITKMKLRAKKGE
                     GAVCAEELEEHAEGLICLTGGVEGPLATALRQGGLEEARWCVDRLLGIFGPGNVYVEL
                     QRHFQREEESRNRAAIDLARSLHLPLLTTNGVNYGTPKARELCDTFTAIRHHRTLSTA
                     GRLLSRNSERFLKSPQEMQELFADLPEALAQTLELSSRLEFTLNDLGYEFPRYSVPEG
                     ETMNSFLRERAWEGFHQRYGRKSQDLQARARLQIEKELKLIEKLKLAGYFLIVWDLVR
                     YCREQNILVQGRGSAANSAVCYSLGITAVDAVSMELLFERFLSEERGEWPDIDLDLPS
                     GDEREKVIQYVYKRYGERGAAMTANVITYRNRMAAREMGKALGFDPETLQKISAAVAT
                     WEFRDENDALDRRFRDAGLDLTHPRLRKYYELCLAVQDMPRHLGQHSGGMVICQGQLD
                     SVVPLEPASMPGRVVVQWDKEDCADMGIIKVDLLGLGMMAVLKDSIELIRDHYREEVD
                     LAHLPQDDSEVYSALQQADTIGLFQVESRAQMSCLPRLRPMRFYDIVVQVAIIRPGPI
                     VGRMVNPFLQRRQGREEVTYAHPSLEPVLKRTLGVPLFQEQLLRIAMIAANFTGGEAE
                     ELRRAMGFKRSQARMKEIEARLRAGMTQNGITPKAQEEIILSITSFALYGFPESHAAS
                     FALIAYASAYLKEHYLAAFTAALLNNQPMGFYSPATIVKDAQRHGLKVLPIDVTRSEW
                     KCTLEQVASRESRAPSASEVVALRMGLRYVRGLREEVGQALVQERLRVPFTSIHDLTR
                     RVPELRKDEVATLAGIGALNFIGLQIDSRSGLNRRDALWQVERAVRASGPLLEQQPEA
                     DSPSPLAPMNHEERLVADFHGTGLTVGPHPMAYRRAWLNAMGIRRASELRDIPSGKRL
                     RIGGCVITRQRPGTAKGFVFISLEDETGVANAIITPDLFHQNRLLLASERFLAIEGIL
                     QNQDNVISVRAERVQPLFVTKAETASHDFH"
     gene            complement(3254..>4460)
                     /locus_tag="VEJ00_05100"
     CDS             complement(3254..>4460)
                     /locus_tag="VEJ00_05100"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXX00570.1"
                     /translation="QAPFTDFRYESPGTTRKITVNDLPEPYVSKSADNGPDVVARPQD
                     AWPVALPGFKVELYAGGLDNPRLLRTAPNGDIFLAESDPGRIRVFRGMTGDGKPEQSA
                     IFASGLKQPYGIAFYPAGPDPQWVYIGNTNELIRFPYHNGDMKASGPSQHIAGLPSTS
                     FGHWTRAIEFSNDGKKLFVAVGSGSNVDDPDTHPSEKDRADILVCDPANCVLSVYASG
                     IRNAGGGIAVNPQTGELWCSVNERDALGDNLVPDYITHVQEGGFYGWPWWYIGPHQDP
                     RHQGKHPELKEKTIVPDVLLQPHNASLELTFYQADKFPAEYKGDIFASEHGSWNKATR
                     AGYEVIRVPLHQTGHATGEYEDFLTGFVTPGGQVWGRPVGITVAPDGSLVVSDDGSNS
                     IWRVSYVGK"
BASE COUNT          937 a         1401 c         1237 g          885 t
ORIGIN      
        1 gcgtgtacgg tgcgccgcgc tttcatttgg cagcacagaa ggcaaaaatc aaggcgcaca
       61 tcggagcgga aattacatgt acccctctca attctgtcat tctgagcgaa gatgctcttg
      121 cgcgaagcgc aagagcatca cagtcgaaga atccctactc ccagcagtcc atgcggaaag
      181 tagggattcc tcgctccgtt cggaatgaca aaccattccg ccttcccctt ctcgtcgctt
      241 cccgcaccgg ttaccagaac ctctgctccc ttatcacaaa gatgaagctg cgcgcaaaga
      301 aaggcgaagg agcggtctgc gcggaagaac tcgaagaaca cgccgaaggc ttgatctgcc
      361 tcaccggcgg cgtagaaggc ccgctggcca cagccttgcg gcagggtggt ttggaagaag
      421 cccggtggtg tgtcgatcga ctactcggga ttttcggccc cggcaatgtt tatgtcgaac
      481 tgcagcgcca cttccagcgc gaagaagaat cacgcaaccg tgctgcgatc gatctcgctc
      541 gctcgttaca tcttccgctg ctcacaacca atggggtgaa ctacggtact cccaaagctc
      601 gcgaactctg cgacacattc acggcgatcc gccaccatcg cacgctttcg accgccggac
      661 gcctgctctc ccgcaactct gaacgctttc tgaaatctcc gcaggaaatg caggaactat
      721 tcgctgatct gccagaagcg ctcgcccaaa cacttgaact ttcttcccgc ctggaattca
      781 cactgaacga cctcggctac gaatttccac gctattccgt gccggaaggc gagaccatga
      841 attcattcct gcgcgaacgc gcgtgggaag gctttcacca gcgctatggc cgcaagtcgc
      901 aagacttgca agcccgcgcc cgcctgcaaa tcgaaaaaga attaaagctc atcgagaaac
      961 tcaagctcgc cggatatttc ctcatcgtct gggacctggt gcgctactgt cgcgagcaga
     1021 acattctcgt ccaaggccgc ggatcggccg ccaacagcgc cgtctgttat tcgctcggca
     1081 tcaccgcggt cgacgccgtc agcatggagc tccttttcga acgcttcctt tccgaggagc
     1141 gcggcgagtg gcctgacatc gatcttgacc tccccagtgg cgacgagcgc gaaaaagtca
     1201 ttcagtatgt ctataagcga tacggcgagc gcggcgcggc catgaccgcc aacgtcatca
     1261 cttatcgcaa ccgcatggct gcgcgcgaaa tgggcaaggc gttgggattc gatccggaaa
     1321 cactccagaa aatttccgct gccgttgcca cctgggagtt ccgtgacgaa aatgacgcgc
     1381 tcgaccgccg cttccgcgac gccggactcg atcttactca tccccgcctg cgcaaatatt
     1441 acgagctatg cctcgccgtg caggacatgc cgcgccacct gggccagcat tccggcggca
     1501 tggtcatctg ccagggacaa ctcgattctg tcgtgccgct cgagcccgcc tccatgcccg
     1561 gccgggtcgt cgtgcagtgg gacaaagaag attgcgccga catgggcatc atcaaggtgg
     1621 atttgcttgg cctcggcatg atggcggtgc tgaaagattc catcgaactc attcgcgacc
     1681 actatcgcga agaagtcgac ctcgcacatc ttccgcagga tgattccgag gtgtactccg
     1741 cgctgcagca agccgacacg atcggactct tccaggtgga aagccgggca caaatgtcgt
     1801 gcttgccgcg cctgcgcccc atgcgctttt acgacatcgt ggtgcaggtc gcgattatcc
     1861 gccccgggcc gatcgtcgga cggatggtga acccgttcct gcagcgccgc cagggaagag
     1921 aagaagtcac gtatgcgcac ccttcgctcg agcccgtgct caagcgcacg ctcggcgtgc
     1981 cgctttttca ggagcaattg ctgcgcatcg ccatgatcgc ggctaatttc accggaggcg
     2041 aagccgaaga actccgccgc gccatgggtt tcaagcgctc gcaggcgcgc atgaaggaaa
     2101 tcgaagcccg gcttcgtgcc ggcatgacgc aaaatggaat cacgcctaaa gcgcaggaag
     2161 aaattattct ttctatcact tctttcgcgc tttacggatt ccccgagtcg catgccgcca
     2221 gtttcgctct catcgcctac gccagcgcct atctgaaaga gcactacctc gctgccttca
     2281 ccgccgcatt gctcaataac cagcctatgg gattttattc accggcgacc atcgtcaaag
     2341 acgcgcagcg acacgggctg aaagtgttgc cgatcgacgt gacgcgctca gagtggaagt
     2401 gcacgctgga gcaagtcgcg agtcgcgagt cccgagcccc gagtgcaagc gaagtcgtcg
     2461 ccctccgaat gggactgcgt tatgttcgtg gcttacgcga agaggtggga caagcactgg
     2521 tgcaggaacg gctgcgcgtt cccttcacct cgatccacga cctcacccgg cgagttcctg
     2581 aactgcgcaa agatgaagtc gcaactcttg ccggaattgg cgcactgaac ttcatcggat
     2641 tgcaaattga ttccaggtcg ggactaaatc gccgcgacgc gctctggcaa gtggaacgag
     2701 ccgtgcgcgc ctctggtccg ctgcttgaac agcaaccaga ggccgattct ccctcaccgc
     2761 tcgcgcccat gaatcacgaa gaacgcctgg tcgccgactt ccacggcact ggactcaccg
     2821 tcggcccgca tcccatggcc taccgccgcg catggctgaa tgctatgggc attcgccggg
     2881 ccagcgaact gcgcgatatt cccagcggca aacggctgcg catcggaggc tgcgtcatca
     2941 cccgccagcg tccgggcacg gcaaaaggtt ttgtctttat cagcctggaa gatgaaaccg
     3001 gcgtggccaa cgccatcatc acgcccgatc tttttcacca gaatcgtctg ctgctggcca
     3061 gcgagcgatt ccttgcgatc gagggaattc tgcagaatca agacaatgtc atttcggttc
     3121 gcgccgaacg cgtgcaacca ctgttcgtca cgaaagcgga aaccgcctcg catgattttc
     3181 actaaaagga ataggggccg gaggctggag aacagggatc agtcaactag tccctgacct
     3241 ctagccctga atctcacttc cccacgtaac tcaccctcca gatcgagttc gatccgtcgt
     3301 cgctcaccac cagcgagcca tcgggcgcga ccgtgatccc cacaggacgc ccccacacct
     3361 ggccgcccgg cgttacgaat cccgtcagga aatcttcata ctcaccggta gcatggcccg
     3421 tttgatgcag cggtacgcgg atcacttcat atcccgcccg tgttgccttg ttccacgatc
     3481 cgtgctcgga agcgaaaatg tctcctttgt attccgcggg aaacttgtcc gcctgataaa
     3541 acgtcagttc cagcgaagcg ttgtgcggct gcagcagcac gtcgggcacg atcgtctttt
     3601 ccttaagttc ggggtgtttg ccctggtgtc gcggatcctg gtgcggcccg atataccacc
     3661 agggccagcc atagaatcca ccctcctgta cgtgcgtgat gtagtcagga acgagattgt
     3721 cgccaagagc gtcacgctcg ttgactgagc accacagttc tccggtctgc ggattgaccg
     3781 cgattccccc gccggcgttg cgaatgccgg aggcatacac gctgagcacg cagttggcgg
     3841 gatcacacac cagaatgtcg gcacggtctt tctcggaggg atgcgtgtct ggatcgtcga
     3901 cattggaccc agaaccgacc gcgacaaata gcttcttacc atcattcgaa aattcgatcg
     3961 cgcgcgtcca atggccaaac gaagttgagg gcaggccggc gatgtgctgt gacggaccgc
     4021 tggccttcat gtcgccgttg tggtagggaa agcgaatcag ttcgttggtg ttgccgatgt
     4081 agacccactg cgggtctggc cccgcaggat agaacgcaat tccgtaaggc tgcttcaggc
     4141 cgctggcaaa aatcgcggac tgctcaggct tgccgtcgcc ggtcattcca cggaagacgc
     4201 ggatgcggcc cggatcgctc tccgccagga aaatgtctcc attcggagcg gtgcgcagca
     4261 agcgcggatt gtccagtccg ccggcataca gttcgacttt gaacccaggt agcgccacgg
     4321 gccaggcgtc ctgcggacgc gcgaccacgt cggggccatt gtccgccgac ttgctgacgt
     4381 agggctcggg caaatcgttc accgtaatct tgcgagtcgt ccccggcgac tcatagcgga
     4441 aatccgtaaa gggtgcttgc
//
LOCUS       DASPUL010000216         9215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_446293, whole genome shotgun sequence.
ACCESSION   DASPUL010000216 DASPUL010000000
VERSION     DASPUL010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9215
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446293"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VEJ00_05105"
     CDS             complement(<1..791)
                     /locus_tag="VEJ00_05105"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXX00571.1"
                     /translation="MRRRYRVWGDALHLAYALVTVAVTSATAYAAPQNDTLSAPRVPS
                     DREKSSFILETMRGGGIPGLQTVVIKNGKVVWHHSYGYSVLAPPGPRKSMRDDSLLFS
                     ASVAKMITVTAVMQQVEKGRISLDDDINKYLPFAIRNPKWPDVPITWRMLLTHTSSID
                     VDQSVEDSVYFYGDDNPITLEEYVEGMFIPGGRYFRADSFRAGRPGTERIYSDNAFDV
                     VGYALARVVKQPFAEYVHDAILVPLQMKESAYSLRKIPADKLSVG"
     gene            complement(1386..2594)
                     /locus_tag="VEJ00_05110"
     CDS             complement(1386..2594)
                     /locus_tag="VEJ00_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HXX00572.1"
                     /translation="MARAQFVWLHRWVGLLMAGFLLLEGITGSLLAYRRSLEELVAPQ
                     LFIAHRPDSPPLPLATLAERAEVLAPEARVGYFWVDDRQAVMRMLPRIKPSVAGSAPL
                     AYDHLFLDPWTGRELGRRRDGDLSQGRLNLVPFIYQLHMNLALGEAGTLVLGIVALAW
                     TLDCFTGFYLTLPLTVSGFLRRWKPAWSVKYPASAIRVNYDIHRASGLWLWPLLFVLG
                     WSSVMFNLPAVYEPVTGKLFDYRSDFELLKGLPLHPNPVPRLTWQDAERRGAKLMERV
                     AAHDRFKVLRPYGMAYIAEFGVYTYAVASDANIEANGWATSLWLDGDTGELVSVDTPR
                     QQPSGNLVGLWLRALHFADIRDSLFYRLLVCVLGLVVAALSITGVYIWLRKRSAKAFV
                     HGRDERAVTL"
     gene            complement(2604..4880)
                     /locus_tag="VEJ00_05115"
     CDS             complement(2604..4880)
                     /locus_tag="VEJ00_05115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HXX00573.1"
                     /translation="MNAFRYCASLAVFCCGVTPLVANADEPQSTTELPKLTVRETVEG
                     DYTVPNASTGTKTDTPILETPLNIQVVTQQVLVDQQVTRIDQALRNVSGVAFTSGGDT
                     SFGNAFDAVVLRGFATDSHLRNGVRIDSVGGDTEFYTQQMANVESVEVLKGPAAILYG
                     AVEPGGVVNIVTKQPQATPSYSIGQQFGSYELYRAVVDATGPLNSASTLLYRIDGSYD
                     TGNSIVDLGFHRDLFIAPTLKLLIGDAAQVTLEYEHKDSNFNGNYAIFPLIQAPSGQF
                     VPLFNDPSQNFGERSTMSEQTDLGGLNWSYQFSPGWTIKQQFLANLVHSSGPQVTTFD
                     GIGPGDPSNPASAPAVYRVEVPFNTHDDTYATYFDLTGHFSTGPVAHTLLFGGDWYRF
                     NSRYVLTTSNPSFVLDPTVDSLISLFDPVHPGTPFGAPQPLAAGTGPTVSWGAYLQDQ
                     VALPDDWFLLAGVRYQHVYASNYSGQTLDSLTSTPISGDKVTPRVGLLWRPLNWVSVY
                     ANYAENWGPSNGYPITGGGLAPPTGATQYELGVKFASADERISSTIAVYDLTKTNIPT
                     PDPNNPGFFLVTGKVRSKGVEFDLQGELARGWNVVVNFSDIDARILESNDPGNPPGTQ
                     WQETPRLIGNLWTTYDTAPTADRGFRFGVGANAQGPTPALNYTGVLAPQTSDYTQLPG
                     FVTLNAMAAYRFTTERIQWMLQLNAANLLDRRYFSYISLNNPQPGATYAYAGNVYGYD
                     RRLYGDPRTLLAQISAQF"
     gene            complement(4925..5518)
                     /locus_tag="VEJ00_05120"
     CDS             complement(4925..5518)
                     /locus_tag="VEJ00_05120"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXX00574.1"
                     /translation="MRYAFVFTIVVLLHLLALAAVWFERVKLPRQEESALTVALLMEP
                     TPLREEKPPEPMPVRLVAPMPAVLVDVPTIEIPLENEIPAPIAPNHPAMPVPVQATTE
                     SVSTELVVQCPERTPPRYPPLAKRQREQGEVRLRVELDENGRIDHVTIVSSSGSPRLD
                     EAARTAIESWRCRPARHDGKPVRAVAMQSLDFVLQPH"
     gene            complement(5609..6118)
                     /locus_tag="VEJ00_05125"
     CDS             complement(5609..6118)
                     /locus_tag="VEJ00_05125"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HXX00575.1"
                     /translation="MIPGIQFKRNGRMIGRFSAIALVFLGLAATLLPVLGAAQAITQV
                     PIQVSGAWARATPPGISVGAAYLLIRNSGPADTLVRIDCPVAESVEMHSSTLEGGVTH
                     MRPVAELALPAHGEIRFAPESLHVMLLGLKSPLVAGQHLRLGLTFKSGARVSADVVVR
                     GLSDDGASG"
     gene            complement(6296..6748)
                     /locus_tag="VEJ00_05130"
     CDS             complement(6296..6748)
                     /locus_tag="VEJ00_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00576.1"
                     /translation="MKNRTALRLAQNPALVGLLLVGFLSRALIPVGFMPGPGGLVVCD
                     GFIHASGAKATPTMEHDMSAMGMDMSGMDMAHRAGHSSKGGGSPEHEHSGICPFAAAA
                     TTMASSHAAVSIAVVPAVARQIDLPSLPFVPRDTIVPTRLPRGPPSLA"
     gene            complement(6952..7422)
                     /locus_tag="VEJ00_05135"
     CDS             complement(6952..7422)
                     /locus_tag="VEJ00_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXX00577.1"
                     /translation="MPQSGKKQSPAGQAATDLILNLFRVNNLLLTVGDRLVDGLGLTS
                     ARWQVLGAIAYAVRPQPVAWLARDMGANRQNVQRIINDLKEAGLVVFQTNPHHRRAQL
                     VVLTDKGKQTFESAMRLQGPWVNRLAQGLAVKDIEIAHRVLTTLRQRLDGKDVA"
     gene            7518..7751
                     /locus_tag="VEJ00_05140"
     CDS             7518..7751
                     /locus_tag="VEJ00_05140"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HXX00578.1"
                     /translation="MLSLRALDPSFPIERQIALETGPVVLVNLFTLDKADEPALLKAW
                     QADAAFMKQQPGFISTQLHRATEESPTYLNYAI"
     gene            7883..8338
                     /locus_tag="VEJ00_05145"
     CDS             7883..8338
                     /locus_tag="VEJ00_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00579.1"
                     /translation="MKRIIHPVAGAIAILTIATFWCSTVLTELSGSKELGAAVKTAIP
                     WGFLLLIPALVAVGASGFVLSKGRRVGLIATKLSRMPLIAANGLLVLIPSALFLASKA
                     RAAEFDSVFYDVQALELAAGAANITLLGLNMRDGLRIKGWLRRRPTGNL"
     gene            complement(8340..9170)
                     /locus_tag="VEJ00_05150"
     CDS             complement(8340..9170)
                     /locus_tag="VEJ00_05150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX00580.1"
                     /translation="MTTALVTGASSGIGTVYARRLAARGHNLVLVARATDRLVKLADE
                     LRDAYCVTVEVITADLTDAPQTDGVTQRLSAEPPIDILVNNAGASVSGGFSHADPAAL
                     QKLLRLNVLAPTLLTAAVLKGMIKRGSGSIINIASVLGLLPEYSHGIYAATKAYVLTM
                     SHSLNAEVASKGIYVQAVLPAATRTEIFQRSGGDISKVPNLMEVDDLVDAALVGFDRK
                     ELVTIPPVPQVADWDAFEHARIVLAQGFSNSKPAERYRRHDAAVRPVACVVSDADVHA
                     "
BASE COUNT         1853 a         2782 c         2758 g         1822 t
ORIGIN      
        1 taccctaccg aaagcttgtc agccggtatt tttcgcagcg agtacgccga ctccttcatt
       61 tgaagcggca cgagaatggc gtcgtgcacg tactccgcga acggctgttt gaccacccgg
      121 gcaagcgcgt acccgaccac atcgaaggcg ttgtcgctgt agatacgctc ggtgcctggc
      181 ctacccgccc taaatgaatc ggcacgaaaa tatcggccgc ccgggatgaa catcccctcc
      241 acatactctt ccaatgtgat cgggttgtcg tcgccgtaga agtacacgga gtcttcgacg
      301 ctctgatcga cgtcaatgct cgaggtatgg gtgagcagca ttcgccacgt aattgggacg
      361 tccggccatt tggggttccg gatcgcaaag ggcaagtact tgttgatgtc gtcatccagc
      421 gaaatgcggc ccttttcgac ctgctgcatc acggccgtta ccgtaatcat tttggcgacc
      481 gaggcactga atagcagcga gtcatcgcgc atcgatttgc gcggtccggg cggcgcaagg
      541 acggaatacc catagctgtg gtgccagacg actttgccgt tcttgattac gacggtctgg
      601 agacctggaa tcccgccgcc tcgcatcgtc tccagtatga acgagctctt ttccctgtcc
      661 gatggaactc gaggcgcaga gagcgtgtcg ttctgcggcg ccgcgtacgc cgttgcggag
      721 gtgaccgcga ctgtcacaag ggcatacgcc aggtgcagcg cgtctcccca aacccgatag
      781 cgcctacgca tgtggactcc ccttcgttcc cgtcttcaac cggcacggct cagcactgcg
      841 ccgagtgcgg gcatgaacgc gatcttacgg atttcgaagt actacgccgt tctagagcgc
      901 ggcggtcgcg aaacgtaaga ctctgctgcg acgatggtca cgatcgtgag cgcttcgccg
      961 cggactattc tacgaccagt tcaccggtag acttccggta gacgttgccg tcgagaacag
     1021 acgcagtttt tcttcagaga gacgcagctc ttcgaggttg tcagctggta accctctggt
     1081 tcccccgagg aaccaccggc taacgagcga ctacgccttc tcccccacct gtaactccgc
     1141 aattttgaag ggcaccccac accctcctat accgcaatac ccatccggat tcttcgcgag
     1201 atattgctgg tgatagtctc cccggaaggt ggaaatccca caagaacccc ggcgttacgg
     1261 tgccgctgat cgtctaccgg tcgactttca gtagacgttg acgtcgatcc agcggtagac
     1321 ctgctcagct tttcccaggc tgtccgctca acggttcaag aatggcaacg ctggcgcgtt
     1381 ccagctcaga gtgtcactgc tcgctcgtcg cgcccatgaa cgaaggcttt ggccgaccgt
     1441 ttgcgaagcc aaatgtaaac tcccgtgatg gacagcgccg ctactactaa ccccaaaacg
     1501 cagaccaaca gccggtagaa cagcgaatca cgaatatcgg caaaatgtag cgcccggagc
     1561 cagaggccca caagattgcc gctgggttgc tggcgagggg tatcgacgct tacgagctcg
     1621 ccggtgtcac cgtcgagcca aagcgaagtc gcccacccgt tggcctcgat gttggcatcg
     1681 ctggcgacgg cgtaggtgta gacaccaaat tccgcgatgt atgccatgcc atacggccga
     1741 aggaccttaa atctgtcgtg cgcagcaacg cgctccatca acttcgcgcc acgccgttca
     1801 gcatcctgcc acgtgagacg tggtaccgga tttggatgca gcggcaaccc cttcagcaat
     1861 tcgaagtccg atcggtagtc aaacagctta ccggtcaccg gttcgtacac ggctgggaga
     1921 ttaaacatca cgctcgacca acccaagacg aacaacaaag gccagagcca aagaccactg
     1981 gcgcggtgga tgtcgtaatt cacccgaatt gcgctcgccg gatacttcac tgaccatgca
     2041 ggcttccagc ggcgaaggaa accgctaacc gtgaggggca aggtcaagta gaaacccgtg
     2101 aaacagtcga gcgtccaggc aagcgcgacg atcccgagca ccaatgtccc tgcctcgccc
     2161 agtgcaagat tcatatggag ctgataaata aacggcacga gattgaggcg cccctgtgac
     2221 aaatctccgt cgcgccgacg tcccagttcc cggcccgtcc acgggtccag aaagagatgg
     2281 tcgtacgcga ggggcgccga gccggccacg cttggcttga tccgtggcag catcctcatc
     2341 acggcttggc gatcgtccac ccagaagtac cctacgcgcg cctcgggcgc cagaacctcg
     2401 gcgcgctcag caagcgtcgc cagcgggagg ggcggagagt ccgggcgatg cgcaataaat
     2461 agctgcggcg caaccagttc ttctagtgaa cgccgatacg cgagcaagct cccggtaatt
     2521 ccctccaaca gcagaaagcc ggccatcaga agaccaaccc accggtgtag ccacacgaat
     2581 tgcgcacgtg ccattgggca cacctagaat tgagcgctga tctgggccag caaggttcgc
     2641 ggatctccgt atagacggcg gtcgtagccg taaacgttgc cagcgtaggc atatgtcgcg
     2701 cctggctgtg ggttgttaag agatatgtaa ctaaagtagc ggcggtccag taaattcgcg
     2761 gcgttgagtt gcagcatcca ctggattcgc tctgttgtga accgatatgc cgccatggca
     2821 ttcagcgtca cgaaccccgg gagctgtgtg tagtcgctcg tctgcggggc cagcactccc
     2881 gtgtaattga gagctggtgt tggcccctgg gcattggcgc cgacgccgaa ccggaagcct
     2941 cggtcggccg taggcgcggt atcgtaggta gtccacaggt taccgatcag ccgcggtgtc
     3001 tcctgccact gcgtgcccgg ggggttcccg ggatcgttgc tctcgagaat ccgggcatcg
     3061 atgtcagaga agttgacaac cacgttccag ccacgggcga gctcgccctg caggtcgaac
     3121 tcgaccccct tgcttcggac tttgcccgtc acgaggaaga acccgggatt gttcgggtcc
     3181 ggcgtcggga tgttggtctt ggtaagatcg tagactgcga tggtcgagga gatccgctcg
     3241 tcggcactcg cgaacttgac gcccagctcg tactgcgtgg cgcccgttgg cggggcgaga
     3301 ccgccgccag tgatcgggta gccgttgctg ggtccccagt tctcagcgta gttggcgtag
     3361 acgctgaccc agttcaacgg gcgccagaga agcccgacgc gaggcgtcac tttgtcgccg
     3421 ctgattggtg tcgaagtgag cgagtccagc gtctggcccg agtagttgga cgcatagacg
     3481 tgctggtagc gaacgccagc gaggagaaac cagtcgtccg gcagggctac ctgatcctgc
     3541 aggtaggcac cccagctgac cgtcggaccg gttccggcag cgagcggttg cggtgccccg
     3601 aaaggcgtgc cagggtgaac cgggtcgaag agactgatta acgagtcaac cgtcggatcc
     3661 aagacaaagc tcggattgga cgtggttagc acgtatctcg aattgaagcg ataccagtcg
     3721 cctccgaaga ggagcgtatg tgccacagga cccgtcgaga agtgtccggt cagatcgaag
     3781 taagtcgcgt aagtatcgtc gtgtgtattg aacggtacct cgacgcggta caccgcgggg
     3841 gcactcgcag gattactggg gtcgccgggc cctatcccat caaaggtcgt cacctgtggc
     3901 ccgcttgagt ggaccagatt tgcgagaaat tgctgcttga tcgtccaacc gggactgaac
     3961 tggtatgacc agttcagccc gccgagatcc gtctgttcgc tcatcgttga gcgctcgccg
     4021 aagttctggc tcggatcgtt gaagagcggt acgaactgtc cacttggggc ttgaatgagg
     4081 ggaaagatcg cgtagttgcc gttaaaattc gaatccttgt gctcgtactc gagggtgacc
     4141 tgggccgcat cgccgatcag cagcttgagc gtcggcgcga tgaacaagtc gcggtggaag
     4201 ccgagatcta cgatcgagtt gccggtgtcg tacgaaccat cgatccggta cagcagcgtg
     4261 ctcgccgagt tgagcgggcc cgtcgcatca accaccgctc ggtatagctc ataggagccg
     4321 aactgctgcc cgatcgagta cgacggcgtc gcctgcggct gctttgtgac gatgttcact
     4381 actccaccgg gctcgacggc accatagagg atggccgcgg gtcccttcag cacctcgaca
     4441 ctttctacgt tcgccatttg ctgtgtgtag aactcggtat cgccaccgac gctgtcgatc
     4501 cgcacgccat ttcgcaggtg ggaatccgtc gcgaaacccc ggagcaccac ggcgtcaaag
     4561 gcattgccga agctcgtgtc gccaccactg gtgaaggcga caccgctcac gttacgaagt
     4621 gcctggtcaa tgcgcgtgac ctgctgatcg accaacacct gctgcgtcac tacttggatg
     4681 ttcagcggcg tctcgaggat tggggtatcg gtcttcgtcc cagtactcgc gttggggact
     4741 gtgtagtcac cctcaaccgt ctcgcgaacg gtcaatttag ggagttcggt cgtcgattgt
     4801 ggctcgtccg catttgcgac gaggggcgtc acgccgcagc aaaacaccgc gagcgaggcg
     4861 cagtaccgaa aagcgttcac gatcgggctc tcccaaatag ttgtgagttt cgttcggcgt
     4921 tgtctcagtg aggctgcagc acgaagtcga gactctgcat agccacagcg cgaacgggct
     4981 tgccatcgtg ccgcgcgggt cggcagcgcc acgactcgat cgccgtgcgc gccgcctcat
     5041 cgaggcgagg ggagccactg ctgctgacga tcgtgacgtg atcgatccgt ccgttctcgt
     5101 cgagctcgac acgaagccga acctctccct gctcgcgctg gcgttttgcc agtggcgggt
     5161 agcggggcgg agtgcgttca ggacactgca ctaccagttc tgtgctaacg ctttcagtgg
     5221 tcgcttggac cggcactggc atcgcgggat gattgggcgc aatcggtgct ggaatctcgt
     5281 tttcgagagg aatctcgatg gtaggaacgt ctacaagaac ggcgggcatg ggcgcgacga
     5341 gacgcacggg cattggttcc ggtggcttct cctcgcgcag gggcgtgggt tccattagca
     5401 gcgcgaccgt cagcgccgat tcctcctggc gcgggagctt tacgcgctca aaccagaccg
     5461 ccgccagcgc gagcagatga agcagcacga ctatggtgaa aacaaaggca tacctcacgt
     5521 ttacccattg gactgaattg cggctggggg gccgccagaa accttcccgc aatggctgac
     5581 gaggcacctt caggcgtcct tggatctgtc acccactcgc gccgtcatca ctgagacccc
     5641 ggaccaccac gtccgctgag acacgcgcgc ctgacttgaa cgtcagcccg agacgcagat
     5701 gctggcccgc aaccagcgga ctcttgaggc caagaagcat cacatgcagc gattcgggtg
     5761 cgaaccggat ctcgccatgc gccggcagcg caagctccgc aaccggtctc atatgcgtca
     5821 cgccgccctc gagcgtgctt gagtgcatct cgacgctctc cgcgaccggg cagtcgatgc
     5881 ggacgagagt gtcggcgggc ccggagtttc ggatcaggag gtaggccgca ccgacagaga
     5941 tgccaggcgg tgtggcacga gcccatgcgc ctgaaacctg gatcggcact tgcgtaatcg
     6001 cctgggctgc tccgagcacc ggtagcagcg ttgccgcgag ccccagaaac acgagagcga
     6061 tggccgagaa acggccgatc atccgtccgt tccgcttgaa ttgaatccca ggaatcatga
     6121 agcggtcctc ccagctcgct ggccttgagc gacggcagcg cccatcacgg ccacacgaac
     6181 aacgcacgtg ctttgtggcc acggtattga ggaaccgtgg aaccgcgacg gcaaaatcac
     6241 tgggcgcacc ttctgtgcgc cgctcaacta gtggttaggt caaccagttg cgtcgtcagg
     6301 cgagggaggg tggcccgcga gggagccgag tgggcacgat cgtgtcgcga gggacgaaag
     6361 gcagggatgg gagatcgatt tgccgagcaa cggcgggaac gacggcaata gagacggcag
     6421 cgtgactgct cgccatagtg gtcgccgcgg ccgcgaaggg acagatgcct gagtgctcgt
     6481 gctcgggtga accgccaccc tttgaagagt ggccggcgcg gtgcgccatg tccatgccgg
     6541 acatgtccat ccccatggcg gacatgtcgt gttccattgt gggggtagct ttggctccgg
     6601 atgcatggat gaagccatca cagaccacca agccgcccgg ccctggcata aatccaaccg
     6661 gaatcaaggc acgggataag aaccccacca gcagcaaccc caccagcgct ggattctggg
     6721 ctaacctgag tgccgtgcgg tttttcacgt cgccagctta atggagtgac ctgcgctttt
     6781 ccatgtaggt cgataacccc caggtcgcgc gtcccgtcgt ttactgtttc cctaccggcg
     6841 ccggtcgacc cgccggcaag ccgtctctgc ccgtgctttc ttctagccca gatatttgtc
     6901 tatacagtcg caggcgcaga gccttgccgt tccagactgc tgttcatgcg atcaggctac
     6961 gtccttgcca tccaagcgtt gacgcaacgt cgtcaacact cggtgcgcga tctcaatgtc
     7021 cttaaccgct aacccctgag cgagtcgatt cacccagggc ccctgtaagc gcatagccga
     7081 ctcaaatgtc tgcttgccct tgtcggtcaa gacgacgagt tgagcgcgtc ggtgatgcgg
     7141 attggtttga aacacgacga gtccagcctc cttcaagtcg ttgatgattc tctgcacgtt
     7201 ctggcgattg gcacccatgt cgcgtgccag ccaagcaacc ggctgcgggc gcaccgcgta
     7261 ggcgattgca ccaagcacct gccagcgggc actggtcaat ccaagcccgt cgaccaatcg
     7321 atcgcccaca gtcagcagca ggttgttcac ccggaaaaga ttgaggatga gatcagttgc
     7381 ggcctggcca gctgggctct gttttttgcc gctttgcggc atcgcggcgc cttacgtctt
     7441 acttgacacg atgatgtcaa tgcactacct atacatcgta ccatattgac atcatcaacc
     7501 catatcagga accaatcatg ctttcgcttc gcgcactcga tccctcattt ccgatcgagc
     7561 gccagatcgc actcgagacg ggccctgtcg tgctggtgaa cctctttacc ctggataagg
     7621 ccgacgagcc ggcccttctg aaggcatggc aggccgatgc agcgttcatg aagcagcagc
     7681 cgggattcat atcgacccag ctgcatcgcg caaccgagga aagcccgacc tacttgaact
     7741 atgcaatatg agaatcgacc gccgccttcc ggaccgcatt ctcccaccca cagttccggg
     7801 ccagccttgc tgcctatccg agctcgacca tcgcctcacc gcacttattt gagagggttg
     7861 ccgtcagcgg catctgcgtt gcatgaaaag gatcattcac ccggtcgcag gcgcgatcgc
     7921 cattctgacg atcgcgacct tctggtgctc gacggttctg acggagctat ccggttcgaa
     7981 agagctcggc gccgccgtca agacagcaat tccatgggga ttcctgttgc tgattccggc
     8041 gctggtcgcg gtaggagcct ccgggtttgt gttgtcgaaa ggacggcggg tcgggctgat
     8101 tgccacgaag ctaagccgca tgccgctgat agccgcgaat ggacttctgg ttctgatccc
     8161 atccgcactc tttcttgcgt ccaaagcgcg agcagccgag tttgacagtg tcttctacga
     8221 cgtgcaggcc ctggagctgg cggcgggcgc cgcaaacatc acccttctcg gcctcaacat
     8281 gcgagatggc ctccggatta agggctggct cagacgccgg ccgacgggaa atctctaaat
     8341 caagcatgta catccgcgtc ggagacgacg catgcaaccg gccttaccgc agcgtcgtgc
     8401 ctgcgatacc gctctgccgg cttagagttg ctaaatccct gcgccaggac tattcgcgca
     8461 tgctcaaaag cgtcccagtc ggcgacttgt ggtaccggcg gaatggtgac gagttccttg
     8521 cggtcaaatc ctaccagggc ggcatcgacg agatcgtcga cttccatcag atttggcacc
     8581 ttgctgatgt cgccgccgga acgctggaag atttcggttc gggttgctgc cggcagaacg
     8641 gcttgcacat agatgccctt ggaggccacc tcggcgttga ggctatgcga catcgtcaac
     8701 acgtacgcct tcgtcgcggc atagatacca tgcgaatatt ccggcaggag gccgagaacc
     8761 gaggcaatgt tgataataga tccgctgccg cgcttgatca ttcccttgag cacggcagcg
     8821 gtcagcaaag taggcgccag tacattgagt cgcaggagct tttgcagggc ggcgggatcg
     8881 gcatgggaaa agccaccgct cacgctggca ccggcattgt tgaccaggat gtcgatcggt
     8941 ggttcggcgc taagtctctg tgttacgccg tccgtttgcg gggcgtctgt caggtccgcg
     9001 gtgatgacct cgacggtgac gcagtacgcg tcccgcagct catcagcaag tttgactagc
     9061 cgatcggtgg cccgggcgac caaaaccaaa ttgtggccgc gtgcagcaag ccgacgtgcg
     9121 tagacagtgc caatgccgct ggaggcaccg gtgactaagg cggttgtcat ggcatgtatc
     9181 ctgatgtgtg aaggctctcg taggtttgct cgtca
//
LOCUS       DASPUL010000217         6222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_860535, whole genome shotgun sequence.
ACCESSION   DASPUL010000217 DASPUL010000000
VERSION     DASPUL010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860535"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VEJ00_05155"
     CDS             <1..370
                     /locus_tag="VEJ00_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00581.1"
                     /translation="TITINTFGPVTTGAVAKKRTRYYAAWLPLPLLAVVGLAAAAGGR
                     RGHKAWGLLALFVVSGSILLAPACGNNTGAQTTTPNGITPNNAYTFTVTGVDINGTTS
                     SNTSGTGATTGNPTVTLTVN"
     gene            388..1140
                     /locus_tag="VEJ00_05160"
     CDS             388..1140
                     /locus_tag="VEJ00_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00582.1"
                     /translation="MHQPMWRRLVVHEAIGLLALSFLFQPFALEPLLGSHKVKLPAVR
                     WNEDTPGCTFSRGDDGKFRYGLWSGDVGVTLAVDSQELEKVRRRHEPFFSVALDVRYR
                     GQQALEVTTDNISLEFSKHFHVVQPSLDPDAFAQKVQDDADAVDHEAAREVQKHPEKK
                     TEKEAYVRAFQKDSTELLEFVSRNSLRPTRLGPENPETHGWVLFNVDSKWISGWKKQE
                     EFILRIPLGDKIFEFPFTLPPTPGEVMLRHRE"
     gene            complement(1141..1551)
                     /locus_tag="VEJ00_05165"
     CDS             complement(1141..1551)
                     /locus_tag="VEJ00_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXX00583.1"
                     /translation="MGTLRELVKDRKVYSIDSARTVLEAARFMMEHNVGALPVLRNGD
                     LVGILSERDIMNRVVAVGRTPGTTAVSEVMTANPRAVPADETIEECLFIMREFGFRHL
                     PIVEGKDLKGLVSLRDVLMHRAAEIEGQTHRAAS"
     gene            complement(1734..3383)
                     /locus_tag="VEJ00_05170"
     CDS             complement(1734..3383)
                     /locus_tag="VEJ00_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HXX00584.1"
                     /translation="MDASPQRGVNPWVVTFSVMLATFMEVLDTTVVNVSIPHIAGNLA
                     STNEEGTWVVTSYLVSNAIVLPISGWLAKYMGRKRLLLTCVAGFTLTSLCCGLATSLP
                     QLIFFRVLQGLTGGGLQPLAQAILLETFPKERHGHAMAAFGIGILLAPILGPTLGGWI
                     TDNYSWRWIFYLNLPVGILSLFLMSRFVFDPYYIKHDGTRVDLWGIGFLALGIGSLQV
                     FLDTGERKDWFSSQYIRTFAVLCVIGLVALVIRELMTDHPVVDLRVLKNRSFSAGVFL
                     ISLLGFILYSSLVLLPLYLQTLMGYPAYNSGLALSPRGIGALLFTPLAGHLTTKTDPR
                     RLLVVGLILGSITMFQLSGLNLYAGFWDILWAQVLQGVALSFLFIPLMTLAMARIPPE
                     RMGNATSIFNLMRNIGGSVGIAIMTTFLARRTQLHQNHLVANVRAGDLQTLRYLAGLR
                     AHFHLLGADSATASRKALGAVYALLQQHAAMLAFVEAFWVMGVVFLLMLPFLPLLQYA
                     KRPPAEAVLEESGAELLKVIEVQPDALSTDIPEEETSVVFH"
     gene            complement(3607..4047)
                     /locus_tag="VEJ00_05175"
     CDS             complement(3607..4047)
                     /locus_tag="VEJ00_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00585.1"
                     /translation="MRSKLALLLILGLASAAYAKDPKHYQSGKLLEMDSVPCGTAEKD
                     AKSFAGEMLGTDSGNKKTQEVLCQEYVLQAEKVIYRIRPHDEKHPVLLPVGADAQFRL
                     QKDKMLLQVEDLDGKEREYSVVSMTPRSDSNSADASATRVNHLQ"
     gene            complement(4243..5547)
                     /gene="aroA"
                     /locus_tag="VEJ00_05180"
     CDS             complement(4243..5547)
                     /gene="aroA"
                     /locus_tag="VEJ00_05180"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HXX00586.1"
                     /translation="MKSESQQLVRPARNVRGSVHLPGDKSISHRYAMLAGIADGPSRL
                     ENYSTGADCASTLACLRALGVKWERDADNAIEVQGRGLSLQPPREPLDCGNSGSTMRM
                     LSGIVAGQRFTSEMIGDESLSRRPMERIITPLSAMGAQITSHQAKPPLRITGGNLKAI
                     EYRMPVASAQVKSCLLFAGLFADGETRVEEPVRTRDHGEVALRAFGAKLERASVGPGS
                     AVRIRGGQRLRGIEARIPGDLSTAAFFLCAAALFPGSQLALPNLLMNPTRARLLDILM
                     QIGLHISVTQLDEVHGEMVGSLQVQGGGLKGVTIAGVDSAAVIDEIPVLAAIAPFTEQ
                     GIEVRDARELRVKESDRIAALAKNLRLMGAQVDERDDGLKIPGGQTLHGAEIDSYGDH
                     RIAMAFAVAALRAEGETLIHGSESAAISYPAFFSTLNDVTER"
     gene            complement(5596..6015)
                     /locus_tag="VEJ00_05185"
     CDS             complement(5596..6015)
                     /locus_tag="VEJ00_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00587.1"
                     /translation="MTTATSEMVLVAAQTVVSAKGDGAAVDVSGASNRVFLVTLAITK
                     IIEQESLDVSIYGSADGAAWGAKSIAAFPQKFYCGESPLLLDLTAHPDVKFVRAHWDV
                     ARWGRGTETPMFEFGVTLRGVPPNILREAAAEAKAMA"
BASE COUNT         1311 a         1908 c         1866 g         1137 t
ORIGIN      
        1 gaccatcacc attaacactt tcggtcccgt caccaccggc gccgtggcga aaaagagaac
       61 tcgttactat gctgcgtggc tgccactgcc gctcctggca gtggtggggt tggctgcggc
      121 ggcgggcgga agacgcggcc acaaggcctg ggggctgttg gctctgtttg ttgtgagcgg
      181 gtccattctg ctggcgcccg cctgcggcaa taacacaggg gcgcagacca ccacgccgaa
      241 tggaatcacg cccaacaacg cttacacctt caccgtgacc ggcgtcgaca tcaacggcac
      301 cacatcgagc aacacgagtg gtactggtgc gacgaccggc aatcccaccg tcaccctgac
      361 ggtgaactag gactcgagct cgaaatcatg caccagccaa tgtggcgtcg gctggttgtg
      421 catgaggcaa tcgggctgct ggcgctgagc ttcctgttcc aaccgtttgc gcttgaaccc
      481 ttgctgggga gccacaaggt caagctcccc gccgtccgct ggaatgaaga cactcccggc
      541 tgcacattct cgcgcggcga cgacggcaag tttcgctatg ggttgtggtc gggggatgtc
      601 ggggttacgt tggccgttga ctcccaggaa ctggagaagg tccgccgccg tcacgaaccg
      661 ttcttcagcg tagctctcga cgtgcgttat cgcggccagc aagcgctcga ggtcactacc
      721 gacaacattt cccttgaatt ctcgaagcat tttcacgtgg tccagccttc tctggatcca
      781 gacgcattcg cccagaaggt gcaagacgat gcggacgcgg tcgaccatga ggcggcacgc
      841 gaggtgcaga aacaccccga gaagaaaacc gagaaggagg cctacgtgcg ggccttccag
      901 aaagattcca ccgagttgct cgagtttgta agcaggaata gtctgcgccc gacgcggctg
      961 ggaccggaga atcccgaaac ccacggctgg gttcttttca acgtcgacag caagtggatc
     1021 agcggctgga agaagcagga ggaatttatt ctccgcattc cgctcggcga caagattttc
     1081 gagtttccgt tcaccctgcc gcccacgccc ggcgaagtga tgctacggca ccgggaatag
     1141 ctaggaggcg gcccgatgcg tctggccctc gatttcagcc gcgcggtgca tgagcacatc
     1201 ccgcagggac accagaccct tcaagtcctt cccctcgacg atgggaaggt gtcggaagcc
     1261 aaactcgcgc atgatgaata ggcattcctc gatggtttcg tcggcgggga cggcgcgcgg
     1321 attcgcggtc atgacttccg acacggccgt ggtgcccggc gtccgcccca ccgccacgac
     1381 gcggttcatg atgtcgcgtt ctgacagaat gcccaccagg tccccgtttc gcagcacggg
     1441 caaggcgccg acgttgtgtt ccatcatgaa gcgggccgcc tccaggactg tgcgagcgga
     1501 gtcgatggaa tagactttgc ggtctttcac gagttcgcgg agagtaccca taaatcactc
     1561 cttttcgcga tggcgcaacc agagcggatt tcgaccagca cccacggcta gccgttacgg
     1621 tgggcaatac tgtatcacag gtttggcttg ctcacgggcc agatcgtacg agatcgcatg
     1681 ttagcgctcc gtggcgccgt gtcacgcaca cgtcgttttg agatagaagc aggtcagtga
     1741 aaaacgacac tcgtttcttc ttccggaatg tcagtcgaca gagcatcagg ctgaacttcg
     1801 atgaccttca agagttcagc accggactcc tcaagtaccg cttccgccgg cggacgtttg
     1861 gcatactgta gcagaggcag aaacggaagc atcagcagaa acaccacacc catcacccag
     1921 aacgcttcca caaacgcaag catggccgcg tgctgctgca acagggcgta gaccgcgccc
     1981 aaagccttgc gcgaagcggt tgcgctgtcg gcgccgagca ggtggaagtg cgcgcggagg
     2041 ccagccagat atctcaaggt ctgcagatcg ccagcccgga cgttggcgac gagatgattc
     2101 tggtggagtt gcgtgcggcg cgccaggaaa gtcgtcatga tggcgatgcc cacactgccg
     2161 ccgatgttgc gcatcaggtt gaagatgctg gtcgcattgc ccattctctc cggcgggatc
     2221 cgtgccatcg ccagcgtcat cagcggaatg aagagaaaac tgagcgcaac accttgaaga
     2281 acttgcgccc acaaaatgtc ccagaaacct gcataaagat tgagcccgga gagctggaac
     2341 atcgtaatcg aaccgagaat caggcccaca accagcaggc gccgcgggtc cgtctttgtg
     2401 gtcaaatggc ccgccagcgg agtgaaaagc aaggctccaa tgcctcgagg tgaaagggcc
     2461 aagccggagt tgtaagcggg ataacccatc agcgtctgca aatacagggg cagcaatacc
     2521 aggctcgagt agagaatgaa tcccagcaag ctgatgagaa acacccccgc gctgaaggaa
     2581 cggttcttga gcacgcgcaa atccaccaca gggtggtcgg tcatcaactc acgaatgacc
     2641 agggcgacca ggccaatgac gcagagcacc gcaaacgtcc ggatgtactg ggatgaaaac
     2701 cagtccttgc gttcgccagt gtcgaggaag acttgcaacg agccaatacc caaggccaga
     2761 aagccaattc cccacaaatc aactctcgtg ccgtcgtgct tgatgtagta cggatcgaag
     2821 acgaaccggc tcatcagaaa cagcgaaaga atccccacgg gaaggttgag atagaagatc
     2881 cagcgccagc tgtagttgtc cgtgatccat ccacccaacg tgggcccgag gatgggcgcc
     2941 agcaggatcc cgatgccgaa agccgccatg gcgtgaccgt ggcgctcctt ggggaaagtc
     3001 tccagcagga tcgcctgggc cagcggctgc aagcccccgc cagtaagccc ttgcaatacg
     3061 cggaagaaaa tcagctgcgg caaggaagta gccaggccgc aacagagtga agtcaaggta
     3121 aatccggcga cgcaggtcag cagcagccgc ttgcggccca tgtacttggc aagccagccc
     3181 gagatcggca gcacaatcgc attcgaaacc agatacgacg tgacaaccca ggtaccctct
     3241 tcattcgtgg atgcgagatt tccggcgatg tgcgggatgg agacgttgac gaccgtcgta
     3301 tccagaactt ccatgaaagt cgccagcatc acggagaatg tgacgaccca cgggttcacg
     3361 ccgcgttgtg gactggcatc cactcggact cgtctctgcc ggcggaggag atccgcgctt
     3421 cctgctcata actatgaatc gcagagcagg atggcgccgt gacaggggtc acaaggagct
     3481 gtcatgcggc ccggtacgag ccattgcaga acgaggcagc cggagacggg aaggcctgag
     3541 gaattgcaag cgaaaacggc gcgggttgta ttccgcgccg tcgcagagct gtgcccttgg
     3601 cttgggttat tgcagatgat tcacacgcgt ggctgaggca tcggcgctgt tgctgtcaga
     3661 acgcggcgtc atcgagacaa cgctatactc gcgctccttg ccgtccagat cttccacctg
     3721 cagcagcatc ttgtcctttt gcaggcgaaa ctgcgcatct gcgccgaccg gcagcagtac
     3781 gggatgcttc tcatcgtgcg gccggatgcg gtagatcacc ttctctgcct gcagtacgta
     3841 ctcctggcaa agaacctcct gcgtcttctt gttgcccgag tccgtcccca gcatctcgcc
     3901 ggcgaagctc ttggcatcct tctcggccgt gccgcaagga accgagtcca tctccaacaa
     3961 cttgcccgac tgatagtgct tgggatcctt ggcataagcc gcagaggcca gccccagaat
     4021 cagcaatagt gccagttttg aacgcatact caacccccga aggaaatagg cgcgaacccc
     4081 acgcgccgca gcggccgcga cacacgcagt cctcggcccg cagcgtattt ttctatggtt
     4141 tggctccatg aaacaggtca ccaatgtcat tggccggaag ttactgcaga agtcggctgg
     4201 aacgggtcgt gcgcctggga taggccttgc cccggaggcg ggctacctct ccgttacatc
     4261 gttcaacgtc gagaagaatg ccgggtagga aatggcggcg gattcagagc catggatgag
     4321 cgtctcaccc tccgcgcgca gggccgcgac agcaaaggcc atggcgatgc ggtggtcacc
     4381 gtaagagtcg atttccgcac cgtgaagcgt ctgccctccg ggaatcttca gcccgtcgtc
     4441 gcgttcgtcg acctgagcgc ccatgaggcg gagatttttc gcgagggcag cgatgcggtc
     4501 ggattctttc acccgcagtt cccgggcgtc ccgcacttca atcccctgct ccgtgaatgg
     4561 ggcgattgcg gccagcacgg gaatttcatc gatcacagcc gccgagtcga caccggcgat
     4621 ggtcacaccc ttcagtccgc cgccttgaac ctgcagcgac ccaaccatct ccccgtgaac
     4681 ttcgtcgagc tgcgtgaccg agatgtgcag cccgatctgc atgaggatat cgagcaggcg
     4741 ggcgcgcgtc gggttcatca gcagattggg gagcgcgagc tgtgaaccgg gaaagagcgc
     4801 ggcagcgcag agaaagaagg cggctgtcga aaggtccccc ggaatgcggg cctcaatgcc
     4861 ccgcaaccgc tgtccgccgc ggatgcgtac cgcgcttccc ggcccgacgc tcgcgcgttc
     4921 cagtttcgcg ccaaacgctc gcagcgccac ctctccgtgg tcgcgcgtgc gcaccggctc
     4981 ttcgacccgg gtctcgccgt cagcgaaaag cccggcaaag agcaggcacg acttcacctg
     5041 cgcgctggcg acgggcatcc tgtattcgat tgccttcagg ttcccgcctg taatgcgcag
     5101 gggtggtttt gcttggtgcg aagtgatctg cgcgcccatg gcggaaagcg gcgtgatgat
     5161 gcgctccatc ggacggcggg acagcgattc gtcgccaatc atctcgctgg taaatcgctg
     5221 tcctgccacg atgccgctca gcatgcgcat ggtcgagccc gagttgccgc aatcgagcgg
     5281 ttcccgcgga ggctgcagcg acaagcctcg cccctgcacc tcgattgcat tgtccgcgtc
     5341 tcgctcccac ttcacgccga gagcacgcag gcaagccagc gtactggcgc aatcggcccc
     5401 agtcgagtag ttctcgaggc gtgaaggacc gtcggcaatg cccgcaagca tcgcgtagcg
     5461 gtgcgagatg gacttgtccc ccggcaagtg cacactgccg cgcacatttc gtgccgggcg
     5521 cacgagctgc tgtgactcac ttttcatctg gaatcaatat aaaggaatgt tcgaatcggc
     5581 tgcggctggt ggtgatcagg ccatcgcctt ggcctcggcc gcggcctctc gcagaatgtt
     5641 ggggggcacg ccgcgcaacg tcacgccgaa ctcgaacatt ggcgtctcgg tgccgcgtcc
     5701 ccagcgcgcg acgtcccaat gcgcgcgcac gaacttcacg tcgggatgcg cggtcagatc
     5761 aagtagcagc ggagactccc cgcagtagaa tttttgagga aaggcggcga tggattttgc
     5821 gccccacgcc gctccgtccg ccgacccata gatgctgacg tcgagcgatt cctgctcgat
     5881 gatcttggtg atggcaagtg ttacaagaaa gacgcggttg gatgcgccgc tcacgtccac
     5941 ggcggcgcca tctcccttgg cactgactac ggtttgcgcg gcaaccagaa ccatttcgct
     6001 cgttgcagtg gtcatcgcga aggattctac caaaaccccc ggccttccac ctttgaagca
     6061 ccagtcgtct cgcggtcgaa aaatgaaaat accggcatcg gcccaatgac agtccgccaa
     6121 cgcggagcta tagtggtcgg cgatggcgag cgcgcaaacc ctgacacggc ctgctgtccg
     6181 agctggtgca tctgcggatg cgccctcgca gcgtctgact tc
//
LOCUS       DASPUL010000218         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_719674, whole genome shotgun sequence.
ACCESSION   DASPUL010000218 DASPUL010000000
VERSION     DASPUL010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719674"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..585)
                     /locus_tag="VEJ00_05190"
     CDS             complement(241..585)
                     /locus_tag="VEJ00_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00588.1"
                     /translation="MSKVSFLQTLGAVWRVAGRHARRSRVLNAAMGAARSTVRSFAHT
                     LHQLWLEVIGTVFLAMAAFGAMALVREYMKYHSGHTTPGRLGIVICFTAMFAWFGVSS
                     FWRVRKSSKQRS"
     gene            783..1079
                     /locus_tag="VEJ00_05195"
     CDS             783..1079
                     /locus_tag="VEJ00_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00589.1"
                     /translation="MDPQLKQLMKELGDAINESLSDSEQISEVVSRIKEGGYDIFLVL
                     EATIGVSKQGEKSPDKTSLVTTLSTNPEFKISDQDLKFLKSLRIKIDEDKQDKK"
     gene            1217..2467
                     /gene="glgC"
                     /locus_tag="VEJ00_05200"
     CDS             1217..2467
                     /gene="glgC"
                     /locus_tag="VEJ00_05200"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HXX00590.1"
                     /translation="MKDTLGVLLAGGAGERLYPLTRDRAKPAVTFGGIYRIIDITLSN
                     CLNSDLRRVYILTQYKALSLNRHIREGWNILGREVGEFIEVLPPMKRVSDQWYQGTAD
                     AVYQNIYSIGSEQPKHVLILSGDHIYKMDYGKMLKQHQDSAADITLATIQVGLEETSR
                     FGVVDVDKDGRINGFEEKPKVTALRSPLNPDKVAASMGIYLFNADVLIPVLLKDAENP
                     QSSHDFGKDILPRMVDDYRVYAYDFVDENKKEARYWRDVGTLEAYYDANMDIVSVSPI
                     FNLYDSEWPIRTHQRQYPPAKFVFAETGRMGTALDSIVSNGCIVSGGLVKNSVLSPDV
                     RVNSYSEVDQCILFSHVSVGRRCRIRKAILDRDVHIPEGTTIGYDAEADRQRYFVTDS
                     GITVVTRDYSLFENPVTVDYFTSE"
     gene            2722..3321
                     /locus_tag="VEJ00_05205"
     CDS             2722..3321
                     /locus_tag="VEJ00_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXX00591.1"
                     /translation="MEDVHVVAMLVRRCIAGDSAAWEEIVQTYNRRIYNVCYRFAGSS
                     DDAEDLTQEVFIKMYRTLSSYDPSKGAFITWVTTIARNLLVDHFRKTKQDRMTDSMDS
                     TASDHEDAQPLSEQIQDQSAPPDAHVRSREVSEAVHTALGKLSPELREAVILRDLQDM
                     DYKEIASVLRVPEGTVKSRINRGRVELARLLQRTYRQVM"
BASE COUNT          743 a         1020 c          981 g          656 t
ORIGIN      
        1 ccgggatatc ccacttcgct ctcgtagtca gaacctttga gatcttcggg agtgtagagg
       61 ggcttgaccg ggatgtgcga ggacgtctgt ggcgctagag gatcagcgcc ggacttgcct
      121 gcgctcggct tcattgccgg atcagcggtc tgcgcgcgat ggctcatgac aacatcccgt
      181 tccctgcaag cttattgcga cggtcgtcgc gaacgcccgc atcggctggc agagctgcga
      241 tcaactgcgc tgcttcgaac tctttctcac tctccagaat gagctgaccc caaaccaggc
      301 gaacatcgcc gtaaaacaga ttacgatgcc caggcggccg ggggtcgtgt gtccggagtg
      361 atacttcatg tactcgcgaa ccagggccat ggccccgaac gctgccatgg ccaggaatac
      421 cgtgccaata acctccagcc acaactgatg cagcgtgtgc gcgaaggagc gcaccgtgct
      481 gcgggccgct cccatcgccg cgttgagcac acggctgcgc cgggcgtgcc gaccggcgac
      541 gcgccagacc gcgcccaacg tctgcaggaa tgaaaccttg ctcacggcct cgattttagc
      601 agggggtggc gctccaatgg cccggtttgc gagaggttgc tctcagcaaa cagatggagc
      661 cgctccggat agggcactag atagggcgtc ggctgcacgg tgtacaccga cggcaaatac
      721 ttaaacgttc gggccattgg cgcctggccc gatgctggta gaatagtcgc gtagggggcc
      781 cagtggatcc gcaactcaag cagttgatga aagaactcgg cgacgccatc aacgaatctc
      841 tttccgattc cgagcagatc tctgaagtcg tctcccgcat caaggaaggc ggatacgata
      901 ttttcctggt gctggaagct acgattggcg tcagcaagca aggcgagaaa tcccccgaca
      961 agacttcttt ggtgaccacg ctctccacga atccggagtt caagatcagc gatcaggatc
     1021 tgaagttcct gaagtcgctc cgcatcaaga tcgacgaaga caagcaggac aagaagtaag
     1081 atccaaactc cgccaagccc tcaagcctga ttgccacctg ggctgtgcta ccatgttttc
     1141 tttttcgcat ctgccgtgcg aaaagcaaga ttccggcgaa agcggcgcgc acaggaccgg
     1201 aatctgaggc gcagtgatga aagatactct cggggtcctt ttggcaggcg gcgccgggga
     1261 gcggctgtat cctctcactc gcgaccgggc caaaccggca gtcaccttcg gcggcatcta
     1321 ccgcattatc gacatcacgc tttccaattg cctgaactcc gacctgcggc gggtctatat
     1381 cctcacgcaa tacaaagcgc tgtcgctgaa ccgccatatc cgcgaaggct ggaacattct
     1441 gggccgcgag gtgggcgagt tcatcgaggt gctgcccccc atgaagcggg tgagcgatca
     1501 gtggtatcag ggcacggccg atgcggtcta ccaaaacatc tattccatcg gatcggaaca
     1561 gcccaagcac gtgctcattc tgtcgggcga tcacatctac aagatggact acggcaagat
     1621 gttgaagcag catcaggact cggcggccga catcaccctg gccacgattc aggtgggcct
     1681 ggaggaaacc tcgcgcttcg gcgtcgtcga tgtcgacaaa gacggacgca tcaacggatt
     1741 cgaagagaag ccgaaagtta cggcgctgcg gtctccgctc aatcctgaca aggtcgccgc
     1801 gtcgatggga atttatcttt tcaacgccga tgtgctgatc ccggtgctgc tcaaagacgc
     1861 agaaaacccc cagtcgagcc acgatttcgg caaggacatc ctgccccgaa tggtggacga
     1921 ctatcgtgtc tatgcttacg actttgtcga cgagaacaag aaagaagcgc ggtactggcg
     1981 cgatgtcggc acgctcgaag cctactacga cgccaacatg gacatcgttt cggtgtcgcc
     2041 gatcttcaac ctgtacgact cggagtggcc cattcgcacc caccagcggc agtatccgcc
     2101 ggcgaagttc gtgtttgcgg aaacgggccg catgggaacg gcgctcgatt cgatcgtttc
     2161 caatggctgc atcgtctccg ggggcctagt gaagaactcc gtcctctcgc ccgacgtgcg
     2221 tgtcaactcc tactcggaag tcgaccagtg cattctgttt tcgcacgtca gtgtcggccg
     2281 gcggtgccgc atccgcaagg ctatcctcga ccgcgacgtg catatccccg agggaaccac
     2341 aatcggctac gacgccgaag ccgaccgcca gcgctacttc gtgaccgaca gcggcatcac
     2401 cgtggtgacg cgcgactatt cgctgttcga gaatccggtg acggtggact acttcaccag
     2461 cgagtagaca atagtctttc ttccgatatc gcccggaact cacacggcag tcccgtgacg
     2521 gtgcagccgc gctgctacaa ttccctgttt cacacgcatg ctcgggttgg ccgcgagctt
     2581 caccgcgaac ttcagggcct acagcgccgt actgtcatgt gggcgaagcc tggagtggtc
     2641 tctcgttgaa ccatcgccag gctgaagctg gtccggcgca agtctcgaag ttcaatacgc
     2701 cagtccttcc ggggagcaca ggtggaagac gtccacgtcg tcgcgatgct ggtcaggcgc
     2761 tgtatcgccg gcgactccgc cgcctgggag gagatcgtgc agacctacaa ccggcgcatc
     2821 tacaacgttt gctaccgttt tgcaggatcc tccgacgacg ccgaagacct cacccaggaa
     2881 gttttcatca agatgtaccg caccctttcg agttacgacc catcgaaagg agcgttcatc
     2941 acctgggtga cgaccatcgc gcgcaacctg ctggtcgacc attttcgcaa gaccaagcag
     3001 gaccggatga ccgactcgat ggactcgacg gcgtccgatc acgaggacgc ccagccgctc
     3061 agtgagcaaa ttcaggacca atcggcccca cccgacgcgc acgtgcgcag ccgcgaggtc
     3121 agcgaggccg tgcacacggc cttgggcaaa ctttcgccag aacttcggga agcagtgatt
     3181 ctaagggact tacaggatat ggactacaag gaaattgcct ctgttttgag ggttccggag
     3241 ggtaccgtga aatcacgaat aaatcgaggg cgggtggaac tagcccggct tctacaacgt
     3301 acctataggc aggtgatgtg atgcctgatc gaacaaataa cggtcagacc agccgtggaa
     3361 tgaactgcaa cgagttcgac ctgctgctta cggacatgct
//
LOCUS       DASPUL010000219         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_446535, whole genome shotgun sequence.
ACCESSION   DASPUL010000219 DASPUL010000000
VERSION     DASPUL010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446535"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VEJ00_05210"
     CDS             <1..778
                     /locus_tag="VEJ00_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HXX00592.1"
                     /translation="AFDFVTKPKDASAHMAETARELIAKVKAAAECKLKPRIRTVAAK
                     PEKVTALPGNPTKVVAIGVSTGGPQAMEYLLSQLPGDFPGAIVVVQHMPEGFTDMFAR
                     RLDEICALRVKEAQSGDVLQPGRVLIGPGSRHLKVKRLPMGDIVVLSDEPRVNGHRPS
                     ADVLLRSVAEEFKNNAVGVLMTGMGDDGAEGLGAVKREGGMTLAQSEESCVVFGMPKA
                     AIERGYALRVVGLEVMGATLQTICGRGMGTDGGRGARAGS"
     gene            840..1343
                     /locus_tag="VEJ00_05215"
     CDS             840..1343
                     /locus_tag="VEJ00_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXX00593.1"
                     /translation="MTGVIWPEGAEHDMQSKGEVGMEQFAPVKRSKDGQPVRYLIVDD
                     SVFARKNLARMIETFGGQVAAEAGDGLTAISEFDRTQPDIVLMDITMPQMEGIEAAER
                     IVQQHPDARVVMVSSVGYQENIVAALQRGARHFVQKPVKPEVLYEVIKYVLGEDGAVA
                     GAAGAGA"
     gene            1347..1844
                     /locus_tag="VEJ00_05220"
     CDS             1347..1844
                     /locus_tag="VEJ00_05220"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HXX00594.1"
                     /translation="MKMELIQPFINAADAVLSQGLQGETKVINLTMEEDAYRRKGVAG
                     MIALTGDIEGRIILDLEPQTAVKVASHYAGAELPESDALVKETIFELANQVVGNAISA
                     LNDQGFHFRVHPPLLLTASEGDKTSEDTEALMICFETPLGNVFMNVALRYNKRRMADH
                     AAVGA"
     gene            complement(2100..>2710)
                     /locus_tag="VEJ00_05225"
     CDS             complement(2100..>2710)
                     /locus_tag="VEJ00_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00595.1"
                     /translation="AIQSEQNTPNLWRFDVASGELKQLTFVKVAQDSSCTPDGKWMVY
                     LGEWVTDNLEHIWKLSTAGGEPVELARGRMNSFPSVSPDSSSVAYTQVEGQGASAKTK
                     FVVQRLEGGTPVQELDAVSDAGPVVGWTPDGRALTYLHTIGSARHLFMQPLTGGAPVQ
                     LTHFDTEPSSIIAWAWSRDGKKIAITRTRFNDTDVVMFSGFR"
BASE COUNT          560 a          768 c          899 g          483 t
ORIGIN      
        1 cgcgttcgac ttcgttacca agccgaaaga cgcttccgcg cacatggcgg agacggcgcg
       61 cgagttgatc gcaaaggtaa aggcggcagc ggagtgcaag ctgaaacctc gcatacgcac
      121 ggttgccgcc aagcccgaga aggtaactgc gcttccgggg aatccgacca aagttgtggc
      181 cattggcgtc tctaccggcg gaccgcaggc catggagtat ctgctgtcgc agctgccggg
      241 agattttccg ggggcgatcg tggtcgtgca gcacatgcca gaaggattta ccgacatgtt
      301 cgcgcggcgg ctggacgaaa tctgtgcgct gcgggtgaag gaagcgcagt ccggagacgt
      361 gctgcagcct ggacgcgtac tgatcggccc gggaagccgg cacttgaaag tcaagcggct
      421 gcccatgggg gacatcgtgg tgctcagcga tgagccgcga gtgaatggcc accgtcccag
      481 cgcggacgtg ctgctgcgca gcgtggccga ggagttcaag aacaatgccg tcggcgtgct
      541 gatgaccggc atgggcgacg acggcgccga aggtttgggc gcggtgaaac gggaaggcgg
      601 catgaccctg gcgcagagcg aggagtcgtg cgtcgtgttc ggcatgccga aagcagccat
      661 tgagcgcgga tacgcgctgc gcgtggtcgg gctggaagtg atgggcgcga cgctgcagac
      721 gatctgcggc cggggcatgg gtacagacgg gggccgggga gcgcgcgccg gcagctagag
      781 tttggaagat acagattagg cacggcttac gccggcgcag cacacgaagg tcggaagcta
      841 tgaccggggt gatctggccg gaaggggccg agcatgatat gcagtcgaaa ggggaggttg
      901 gtatggagca gtttgcgcca gtaaaacgca gcaaggacgg gcagccggtc cgctatctga
      961 tcgtggatga ctccgtgttt gcccgcaaga acctggcccg catgatcgaa acgttcggcg
     1021 ggcaggtggc ggccgaggcc ggcgacggct tgaccgccat cagcgagttc gaccgcacgc
     1081 agcccgacat cgtgctcatg gacatcacca tgccgcagat ggagggcatc gaggcggctg
     1141 aacggatcgt gcagcagcat ccggacgcac gcgtggtcat ggtgtcgtcg gtgggctacc
     1201 aggagaacat tgtggcggcg ctgcagcgcg gcgcgcgcca ttttgtgcag aagccggtga
     1261 agccggaagt gctctacgaa gtcatcaagt acgtgctcgg cgaagacggt gccgtagccg
     1321 gcgccgcggg agcgggagcg taagcaatga agatggaatt gatccagcct ttcatcaatg
     1381 cggccgacgc cgtgttgtcg caaggcctgc agggcgagac caaggtgatc aacctcacca
     1441 tggaagagga cgcctatcgc cgcaagggtg tggccggcat gattgccctg accggcgaca
     1501 tcgagggccg gatcattctc gatctcgagc cgcagaccgc ggtcaaggtt gccagccact
     1561 acgccggcgc agaattgccc gagtccgacg cgctggtgaa ggaaaccatc ttcgaactgg
     1621 ccaaccaggt cgtgggcaac gccatctcgg cgctgaacga ccagggcttc cacttccgcg
     1681 tgcatcctcc gctgctgctc accgcctccg aaggcgacaa gaccagtgaa gacaccgaag
     1741 cgctgatgat ctgcttcgag acccccctcg gcaatgtctt catgaacgtc gccctgcgct
     1801 acaacaagcg gagaatggcc gatcacgccg ctgtgggagc gtgacggcag gggtcagtag
     1861 caggagccag tttccatgcc tttcatcttg tctgtgtcca tgagacgcta gccggttgag
     1921 atatcggccc cttcgagctc accttccgaa tctctgttta ttggtgaaac agtcaatgag
     1981 cactattacc tgttaatggt ggagcgcaga ttttccgcac cagggatttt ggagccttgc
     2041 aacgcaaaaa tcggtagcgc ggattacggc acggaagaca ggagtgaggc gagcggcggt
     2101 caccggaaac cgctgaacat aaccacgtcg gtatcgttga aacgggtgcg ggtgatggcg
     2161 atctttttgc cgtcgcgcga ccaggcccac gcgatgatgg aagaaggctc ggtatcgaag
     2221 tgagtcaact ggaccggcgc gccaccggtc agcggctgca taaaaaggtg cctcgcgctg
     2281 ccgatcgtgt gcaaataagt cagcgcgcgg ccatcaggag tccatcccac gactggaccg
     2341 gcgtctgaca cagcatcgag ttcctgtacc ggagtgccac cctccagtct ttgcacaacg
     2401 aactttgttt tcgcacttgc tccttgtccc tcaacctgcg tgtaggccac ggatgaacta
     2461 tcgggcgaga cggacggaaa gctgttcatc ctcccacggg ccagctccac cggctcaccg
     2521 ccggcggtcg agagtttcca gatgtgctcc aggttgtcgg tcacccattc tcccagatac
     2581 accatccact tcccatctgg agtgcaggag gaatcttgcg cgactttcac aaaagtgagc
     2641 tgtttcagct cgccggatgc aacatcgaat cgccagagat tcggtgtgtt ctgctcggat
     2701 tggatggcga
//
LOCUS       DASPUL010000220         7353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1268015, whole genome shotgun sequence.
ACCESSION   DASPUL010000220 DASPUL010000000
VERSION     DASPUL010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268015"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VEJ00_05230"
     CDS             <1..500
                     /locus_tag="VEJ00_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00596.1"
                     /translation="LATRVFEIGGGKVEIFPGNYEDYLWRKEGGQQVTPTLDDVPGAT
                     AAKSQPLGAPLLPDFGRSGQQPGASDQPTNGNHSAERDLPEQTKKRLNPIKRKQMQDR
                     VHELEAEISRTEAAIANLEAALQNFVSAEESQRQSQELERHKVTNAALIKEWESLAAS
                     LQESE"
     gene            1026..1535
                     /locus_tag="VEJ00_05235"
     CDS             1026..1535
                     /locus_tag="VEJ00_05235"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXX00597.1"
                     /translation="MLQSSDNSYNLKSGSPASNPNAYNPVKTVTAPVDQATIGRTLFI
                     KGEISGSEALYIDGRIEGKITMPESRVTIGRNGKVDASITAREVVVMGKVTGNIECSD
                     RVDIRSEGSVHGDISTVRISVEDGASLKGGIQVRNEQKHKEIPAQAQSQTPAKQPDLP
                     KAMAATASA"
     gene            complement(1759..2175)
                     /gene="msrB"
                     /locus_tag="VEJ00_05240"
     CDS             complement(1759..2175)
                     /gene="msrB"
                     /locus_tag="VEJ00_05240"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXX00598.1"
                     /translation="MTEKTAEKVTKTEAEWRKQLSPEQYHVTREAGTECAFTGAYWNN
                     HEPGVYHCVCCGAPLFDSQTKFNSGTGWPSFYKPTSDGGVIEHRDSSYGMVRTEVRCA
                     KCDAHLGHVFEDGPAPTGLRYCMNSAALDFRAKEKK"
     gene            complement(2344..2844)
                     /locus_tag="VEJ00_05245"
     CDS             complement(2344..2844)
                     /locus_tag="VEJ00_05245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXX00599.1"
                     /translation="MSLNSAEFRQAMGCFATGVTIITVDLDGEVHGMTANAFASVSLD
                     PMLVLVCVDRDTRTHAHLHARKRFGINVLGEDQRVISEYYARVDRSHEHAETEAGARF
                     ERTRHGTPMLHGSLAYLECRLHSAQAAGDHTIFIAEVEDVAVRGGDPLLFFRGKYRKV
                     GEEVER"
     gene            2901..4103
                     /gene="cofG"
                     /locus_tag="VEJ00_05250"
     CDS             2901..4103
                     /gene="cofG"
                     /locus_tag="VEJ00_05250"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HXX00600.1"
                     /translation="MTLTATTPQLRSACAAALAAVRPGEPISREAALLLIHCPDADLA
                     ELLSAARAAKERFKPDVITYSRKVFIPLTNLCRDYCGYCTFRRDPGDPGAHTMTPEEV
                     LAVARAGEKLGCTEALFSLGDKPELLFFQMRETLRRLGYRSTLHYLEAMCELVLRETS
                     LLPHPNPGLLSPEWIARLAEVSPSMGLMLETTNESLLAPGAAHDNAPDKVPAKRLRTI
                     EDAGKQKVPFTTGLLVGIGESPEDRVDTLLAIRDLHDRYGHIQEVIVQNFRVKPSIPM
                     AHWAEPTRRDMLRAVAVARLLLPKVNIQAPPNLSAPYYEELADAGINDWGGISPVTPD
                     YINPEKPWPHVEELHLRTEAKGCKLRQRLPVYPEFLPALAAKSGLLSERLRTASDDHG
                     LAKLERAA"
     gene            4117..5103
                     /gene="cofD"
                     /locus_tag="VEJ00_05255"
     CDS             4117..5103
                     /gene="cofD"
                     /locus_tag="VEJ00_05255"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HXX00601.1"
                     /translation="MICVLSGGTGGAKFVDGLRQVMPAEDITIVVNTGDDLMWWGLYV
                     SPDIDSITYVLSGMLSRERGWGVKGDTFLCLQAMGQLGEPTWFHAGDRDLAVHLLRSR
                     LLAEGKTLSEATSIICGKLGVRARILPMSNSRVETRVDTPVGELSFEEYFVQRWYQDP
                     VKSVRFAGASDAEPAPGVIDAITSADAVLIAPSNPITSIGPILAVPGVREALQRARGK
                     IAAVSPIVGNAPVAGPAGILMAAQGLPCSIAGVAQAYDDFLDILICDTRDARAAESLR
                     QGGLRVQCTQTIMRSADDKAALAREVLSFACGGLLREEPAATTAFRRLDREP"
     gene            5268..5924
                     /gene="cofC"
                     /locus_tag="VEJ00_05260"
     CDS             5268..5924
                     /gene="cofC"
                     /locus_tag="VEJ00_05260"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:NF014085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HXX00602.1"
                     /translation="MILIPVKDLSLAKQRLASVLDQPSRTALAQAMLDDVLATLHGWK
                     NRPQVGIVTSDPHALELARKYEFEIIPDPDNPGETGAIEMATRIAVERGAEFTLVIPA
                     DIPLIQAWELEEIFKHAPAQGSVLVPAADGRGTNAAFRRPADLFALRFGNDSFKPHHA
                     TAEATGKPCVVLNLPGIAVDVDNPEDLHRLISLPGETRAQILVRKYARAGQSMEPQTT
                     "
     gene            6025..6807
                     /gene="cofE"
                     /locus_tag="VEJ00_05265"
     CDS             6025..6807
                     /gene="cofE"
                     /locus_tag="VEJ00_05265"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HXX00603.1"
                     /translation="MRRKVPTPSATAELRIIPIPLAAEIHPGDRLADKLLESLRRRRS
                     RLRSGDILLVKHKIVSKSEGRLVDLATIQPSAESEAWAKQYNLDARVIELALREARSV
                     IRRKNGVLITETHHGFLCANSGVDVSNVDGGRHALLLPKDPDRSAANLRRALKKRTGL
                     AIPVIITDSFGRPWREGLTDFAIGIAGMKPLRDDRGRRDLHGYKLQASVEASADELAS
                     AAGLVCGKLNRTPACIVRGFRYDAGSGGVRNLLRPAATDLFR"
     gene            6940..>7353
                     /locus_tag="VEJ00_05270"
     CDS             6940..>7353
                     /locus_tag="VEJ00_05270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXX00604.1"
                     /translation="MPRELTASELEAFPSLDWSDIAPRLTPPFRSSLEKVLETQNGAN
                     LSLDESYTLANAEGDDLLGLLVAANLLRAELAGNIVTYVVNRNINFTNICFVGCKFCA
                     FSRGPREADAYFHTLDDMARKAREAWQLGATEVCIQ"
BASE COUNT         1486 a         2550 c         1996 g         1321 t
ORIGIN      
        1 agctggccac gcgcgtcttc gaaatcggcg gcggcaaggt cgagatcttc cccggcaact
       61 acgaagacta tctctggcgc aaggaaggcg gacagcaggt gacgcccaca ctggatgacg
      121 tcccgggcgc aactgcggcg aaatcacagc cgttgggtgc cccacttctc cccgattttg
      181 ggagaagtgg gcaacagcca ggagcgtccg atcagccgac aaacggcaat cacagcgctg
      241 aaagggattt gccggagcaa accaaaaagc gcctgaaccc catcaagcgc aagcagatgc
      301 aagaccgcgt ccacgaactg gaagcagaaa tcagccgcac cgaagccgca atcgccaatc
      361 tcgaagccgc gttgcagaat ttcgtcagcg ccgaggaaag tcagcgccag tcgcaggaac
      421 tcgagcggca caaagtgaca aacgctgcct taatcaagga gtgggaaagc cttgccgcca
      481 gcctgcaaga atccgagtag gcatcgtgag cagaatcgaa gttgccgcag cccgatgatg
      541 acgtccctaa tttcattaaa agctggccca ccgctgtctt attccgttca gattctgcca
      601 agcgatattg cgttaccccg gagggaccgc tcacaatatc ccgccagttt actggcggga
      661 aacgtgtgct cttgattttc gtcccgtgcc ggaggctcgg ctgaaccatc tgtaccggcg
      721 tttcaggcgt ccttccggga cgcggttttt ccttcggtcc ccacccagca ttgaaaacgc
      781 tggactattt tcaggcgtcc ctaagggacg aacacatccc atcgactcac aggggttcta
      841 gacttgtcgt cgccaactcc agcccgccgc ccgtacacca tcttccgtaa tctcactctc
      901 cccttacctc tgtgcgatat tcaacttggc caagtggcca gcaactgccg aaggccacaa
      961 atttcacaga ccagccgctt ccgggcgccg gccgagggcg ccgcgcggac accaaggaag
     1021 gtcgcatgct tcagtcgtcg gacaactctt ataacctgaa gtccggctca ccggcttcca
     1081 accccaacgc ttacaacccc gtcaagactg tgaccgcgcc cgttgaccag gccaccatcg
     1141 gccgcacgct gttcatcaag ggcgaaatca gcggctccga agcgctctac atcgacggcc
     1201 gcatcgaagg caagatcacc atgcccgaaa gccgtgtcac cattggccgc aacggcaaag
     1261 tagacgccag catcaccgcc cgcgaagtcg tcgttatggg caaagtcacc ggcaacatcg
     1321 agtgcagcga tcgcgttgat atccgcagcg aaggctcggt ccatggcgac atttccacgg
     1381 tccgcatcag cgtggaagac ggcgcctcgc tcaagggcgg gatccaggtg cgcaacgagc
     1441 agaagcacaa agagattccg gcacaggctc agtcccagac cccggccaag cagcccgacc
     1501 tgcccaaggc catggcagcc acggccagcg catagttttt cctcgttggg tcgcaataaa
     1561 agacaaggca cgcggaagcg tgcctttgct ttttcgacaa cagaaagtga agatttccct
     1621 tacgtctctg atgccgacgc cgttcggcga aaaacctgat caatgccggg acaccaccgt
     1681 cacgcggaag agcggccctt cagggccgcg taaggggcca gtaagggacg gggctttagc
     1741 ccctgagggc tgccgcccct attttttctc cttcgccctg aaatccagag ccgccgaatt
     1801 catgcaatag cgcagccccg tgggcgcggg tccatcctca aacacgtgcc ccagatgcgc
     1861 gtcgcacttc gcgcagcgta cctcggtccg caccatgccg tagctcgaat cgcgatgctc
     1921 gatcacgccg ccgtcgctgg tcggcttgta gaaactcggc cagcccgtcc cggaattgaa
     1981 cttcgtctgc gagtcaaaca gcggagcccc gcagcacacg cagtgataca cgcccggttc
     2041 gtggttgttc caatacgccc cggtaaaagc gcactccgtg cccgcctcgc gcgtcacgtg
     2101 atattgctca ggcgaaagct gcttccgcca ctcggcctca gtcttggtga ctttctctgc
     2161 agtcttctca gtcatagata aaactcccat gagatcggta aatactgatt actagatgtt
     2221 cggttgggca aaaagtgaca tcgaacgctc acgctttcct ccatctggaa accggcgtta
     2281 tcccgaaggc ccgtgccttc accagcgggc cgagggatct cccagccccc agccgtccac
     2341 aaattacctc tccacttcct cccccacctt ccgatacttg ccccgaaaaa acagcagcgg
     2401 atcgcccccc cgcacggcca catcctcgac ctcggcgatg aagatcgtgt gatctccggc
     2461 cgcctgcgcg gagtgcagcc ggcactccag atacgccagc gatccgtgca gcatcggagt
     2521 cccatgcctg gtccgctcaa accgtgcccc ggcttccgtt tcagcgtgct cgtggctgcg
     2581 gtccacccgc gcgtagtatt ccgaaatcac tcgctgatcc tcgcccagca cgttgatgcc
     2641 gaaacgcttc ctggcgtgca gatgcgcgtg cgtgcgcgtg tcgcgatcca cgcacaccag
     2701 caccagcatg gggtcaagcg acaccgaggc aaaggcattt gcggtcattc cgtgcacttc
     2761 cccgtcgagg tcgacggtga tgatcgtcac gccagtggcg aagcatccca tcgcctggcg
     2821 aaattcggca gaattcaagc tcatcgaaaa accgcccgcc tacattacca cggcgccgcg
     2881 ttgtatcatt cgattcaacc gtgaccctga ccgccaccac gccgcaactg cgctctgcct
     2941 gcgccgcagc gctcgcagcc gtgcgccccg gcgagcccat ctcgcgcgaa gcggctctcc
     3001 tcctgatcca ctgccccgac gccgacctcg ccgaactcct ctccgccgcc cgtgcagcca
     3061 aagagcgctt caagcccgac gtcatcacct attcacgcaa ggtctttatc ccgctgacca
     3121 atctttgccg cgactactgc ggatactgca cctttcgccg cgaccccggc gacccaggcg
     3181 cgcacacgat gacgcccgag gaggtgctcg ccgtcgcccg agccggcgag aaactcggct
     3241 gcaccgaggc cctcttcagc ctcggcgaca agcccgagct gcttttcttc caaatgcgcg
     3301 aaaccctccg ccgcctcggc tacaggtcta cgctgcacta tctcgaagcc atgtgcgagc
     3361 tcgtgctgcg cgagaccagc ctgttgccgc atcccaatcc cggactgctc agccccgaat
     3421 ggatcgcacg tctggccgag gtctcgccca gcatgggcct tatgctcgaa accacgaacg
     3481 agagtcttct tgcacccggc gctgcccacg acaacgctcc cgacaaagtt ccggccaagc
     3541 gtctccgcac catcgaagac gccggcaagc agaaagtccc gttcaccacc ggcctgctcg
     3601 tcggcatcgg cgaatcgccc gaagaccgcg tcgacacgct gctcgcgatc cgcgatttgc
     3661 acgaccgcta cggccacatc caggaggtta tcgtccagaa cttccgcgtg aaaccgtcga
     3721 tccccatggc gcactgggcc gagcccaccc gccgcgacat gctgcgcgcc gtagcggtag
     3781 ctcgcttgtt actccccaag gtcaatattc aggcgcctcc gaacctttcc gcgccttact
     3841 atgaagagtt ggcggacgcg ggcatcaacg attggggagg gatttcaccg gtgacccccg
     3901 actacatcaa tcccgaaaag ccctggccgc acgtcgagga actgcacctt cgcaccgagg
     3961 ccaagggatg caaactgcgc cagcgcctgc ccgtctatcc cgagtttctt ccggcgctcg
     4021 ccgccaagtc tggactcctg tcagaaagat tgcgcaccgc ctccgacgat cacggcctgg
     4081 cgaaactgga gagagcggca tgatccggag gaagaaatga tctgcgtcct cagcggcggc
     4141 accggcggcg ccaagtttgt cgatggcctg cgccaggtca tgcccgcaga agacatcacc
     4201 atcgtcgtca acaccggcga tgacctgatg tggtggggcc tttatgtctc gcccgacatc
     4261 gactcgatca cctacgtcct atccggcatg ctcagccgcg agcgcggatg gggcgtgaaa
     4321 ggcgacacct tcctctgcct gcaggccatg ggacaactcg gcgagcctac ctggtttcac
     4381 gccggcgacc gcgacctggc cgtccatctg ctgcgctcgc gtttgctggc cgagggcaag
     4441 acgctctccg aagcgacctc catcatctgc ggcaaactcg gggtcagggc gcgcattctg
     4501 ccgatgagca actcgcgcgt cgaaacccgc gtggatacgc cggtcggcga gttgagcttt
     4561 gaagaatatt tcgtgcagcg ctggtaccag gatccggtca agtccgttcg cttcgccggc
     4621 gcttccgatg ccgagcccgc acctggcgtc atcgatgcga tcacttccgc tgatgccgtg
     4681 ttgatcgcgc ccagcaaccc catcaccagc attggaccca ttcttgccgt gcccggcgtg
     4741 cgcgaagcgc tgcagcgcgc tcgtgggaaa atcgctgccg tcagccccat cgtgggcaac
     4801 gcaccggtcg cggggccggc tgggattctt atggctgctc aaggcttgcc ctgctcaatc
     4861 gccggagttg cccaagccta cgacgacttt ctcgacatcc tgatctgtga cacgcgcgac
     4921 gcccgcgccg ccgagtctct tcgccaggga ggcctgcgcg tgcagtgcac gcaaacgatc
     4981 atgcgcagcg ctgacgacaa agccgcgctc gcgcgcgaag tgctctcttt cgcctgcggc
     5041 ggcctgttgc gggaggaacc cgcggccacc acggctttcc gccgcctgga ccgcgagccg
     5101 tgatcctcgt atggcgcgaa ggagtaagcc aggagcgaat gacgcgggaa gaaacgaacc
     5161 gggaagggca cgacttcagt catgccgata ggaatccacg tacaacaggg cttcagcccc
     5221 tgagggtcgc ggttccccgt gtgcgcagca tgcgataagc tcttcacgtg attctcatcc
     5281 cggttaaaga tctgtcctta gccaaacagc gcctggcctc ggtcctcgat caaccctcgc
     5341 gaactgccct ggctcaagcc atgctcgacg atgtcctcgc cacccttcac ggctggaaga
     5401 atcgtccgca ggtcggaatc gtgaccagcg atccccacgc actcgaactc gcgcggaaat
     5461 acgagttcga gatcatcccc gatcccgaca atcccggcga gaccggtgcc atcgagatgg
     5521 ccactcgcat tgccgtcgag cgcggcgccg aatttacgct tgtgatcccg gctgatatcc
     5581 cgttgattca agcgtgggaa ctcgaagaaa tcttcaagca cgcacccgcc caaggcagcg
     5641 ttctcgtgcc cgctgccgac ggacgcggca cgaatgccgc cttccgccgg cccgccgatc
     5701 tgttcgcgct gcgcttcggc aacgacagct tcaaaccgca tcacgccacc gccgaagcca
     5761 ccggcaagcc ttgcgtcgtc ctgaatctcc ccggcatcgc ggtcgacgtc gacaatcccg
     5821 aagatctcca ccgcctcatt tcgctccccg gcgaaacccg cgcgcagatc ctggtccgca
     5881 aatacgcgcg agcagggcaa tccatggaac cacagaccac gtaaggatag ccacacagtg
     5941 ggatttccag acagttcctc ggcctccagg cagatacact gttgattgat gggcaatcgc
     6001 gccacaacca ggagttcaac cgtcatgcgg cgcaaggtac cgacaccttc agcgacggct
     6061 gaacttcgaa tcatcccgat ccccctcgcc gccgaaatcc atcccggaga ccgcctcgcc
     6121 gacaagttgc tggaatcgct acgccgccgg cgcagccgcc tccgatcggg cgacatcctg
     6181 ctcgtcaaac acaaaatcgt ctccaagtcc gaaggtcgcc tcgtggacct ggccacaatc
     6241 cagccctccg ctgaatcgga agcctgggcg aagcaataca atctcgatgc ccgcgtcatc
     6301 gaactcgctc tgcgcgaggc ccgctcggtc atccgccgca agaatggcgt tctgattacc
     6361 gaaacgcatc acggatttct ctgcgccaac agcggtgtcg acgtttctaa cgtcgacggc
     6421 ggccgccacg ctctgctgct tcctaaagac cccgaccgct ccgccgccaa cctgcgccgc
     6481 gcgttgaaaa agcgcaccgg cctcgcgatt cccgtcatca ttaccgacag cttcggacgc
     6541 ccatggcggg aaggcctgac cgacttcgcc atcggcatcg ctggcatgaa gccgctgcgc
     6601 gacgaccgcg gccggcgcga cctccacggc tacaaactgc aggccagcgt cgaagccagc
     6661 gccgacgaac tcgccagcgc cgccgggctg gtgtgcggca aactcaatcg cacgccggcc
     6721 tgcatcgtcc gcggatttcg ctacgacgct ggctctggcg gagtgcggaa tcttctccgc
     6781 cctgcagcta ccgacctgtt ccggtagact gttttggaca gtcccgtgcc tggacaattt
     6841 tgcttgaatg attcttgttt ttgggtggcg cagcgctttt agcgctgcga taagaaaatt
     6901 gattgaagag ttgggcttta gcccttgagg tttgaggaca tgccccgcga acttacagct
     6961 tcggaattgg aagccttccc gtcgctcgac tggtccgaca tcgcgccgcg cctgactccg
     7021 cctttccgct cttcactcga aaaagttctg gagacccaga acggcgccaa cctctcactg
     7081 gacgagtctt acacgctggc caacgccgaa ggcgacgatc tcctgggtct tctcgtcgcc
     7141 gccaaccttc tccgcgctga actcgccggc aacatcgtga cctacgtagt caaccgcaac
     7201 atcaatttca ccaacatctg tttcgtcgga tgcaaattct gcgccttcag ccgtggcccg
     7261 cgcgaggccg acgcctactt tcacacgctc gacgacatgg cccgcaaagc ccgcgaagcc
     7321 tggcaactgg gcgccaccga agtctgcatt cag
//
LOCUS       DASPUL010000221         6201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1816221, whole genome shotgun sequence.
ACCESSION   DASPUL010000221 DASPUL010000000
VERSION     DASPUL010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816221"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1682)
                     /locus_tag="VEJ00_05275"
     CDS             complement(<1..1682)
                     /locus_tag="VEJ00_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXX00605.1"
                     /translation="MTISRREFVVRGAAAGTGLVIGVRLSVPFALAQENAKARKKPAP
                     NPFDAWIHVKPNGQISLIVAKSEMGQGIKTGLAMVLAEEAEVDFNSVSVEQAETRPDI
                     YPHLGTGGSSSTRENFTPLRQAGATVRELMITAAAAKWNVPRGQCQARKGAVLHAASS
                     RQAGYGELVDAAAKLPRPDPEKVPLKDPSKFELIGHATPRVDIPSKVNGRAQFGLDVR
                     VPDMLFAVVARCPTFGGKAAHFETAKAKAVSGVRDVFEIPAMGADMFTAGGVAVIADS
                     TWAAMKGRDALDITWDHGPAVSESNDSLRKSLLAAAQKPGKRVRNDGDVDAALAHAAK
                     PVEALYELPFLAHATMEPMNITVHARGGEAEVWAPTQSPDWVQGTVAKVLGITPQKVT
                     VHTTFMGGGFGRRYMADFPAEGAQIAKVVGKPVQLVWSREDDMTHDFYRPASCHRFRG
                     AIDAQGRPTAWFHTLVSTAIQGYWDPKKPPEDDEIGGAKQIPYAIPNIRLEYSPVASA
                     VPRAWWRSVENSFNGFAVESFIDELAAAAGQDPFEFRKSLLVKPPNWKPKDE"
     gene            complement(1685..2155)
                     /locus_tag="VEJ00_05280"
     CDS             complement(1685..2155)
                     /locus_tag="VEJ00_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXX00606.1"
                     /translation="MAAFHLTINNSPHDTDVSPDTPLLWVLRDKLNMTGTKYGCGLGL
                     CGACTVHLDGEAVRSCQIAVSDVGKKKITTIEGLSTDNSHPVQLAWIAEQVPQCGYCQ
                     CGQIMSAAALLAKTPHPSDDQIDDAMVGNLCRCGMYQRIRRAIQRASKSAAQGA"
     gene            complement(2269..2640)
                     /locus_tag="VEJ00_05285"
     CDS             complement(2269..2640)
                     /locus_tag="VEJ00_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00607.1"
                     /translation="MSTIGDVCGPVGEGSYGLMQIKNEDCSGTVIHGGYPATSQSTAL
                     NVDWYAAHIRSCYDGDFYDGGQWLYLGQTVDQIAAQHGWDYVFWACIGAHFSGNWQPG
                     QPYQLRVQQTLANRTWEQPGF"
     gene            complement(2858..3517)
                     /locus_tag="VEJ00_05290"
     CDS             complement(2858..3517)
                     /locus_tag="VEJ00_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00608.1"
                     /translation="MAIAVIHQMSLRCSRPKARSAAGALALLAILLGCGGQPTASNQP
                     PKTPPTKPGYFGTEPSHATFLPRSDAYCASAVTPNSYEPRPDNDQANHTVLSPPYNWS
                     VENYWTKWRDKRAQVTGNCTGTTTEIIQWAACKWGIDEDTIRADAVIESYWHMSTIGR
                     RRTGAASHSAGLRRPANDRQSASEDSSHQTRLLRYRACPLDVSSPLRCLLRFGGNAQL
                     V"
     gene            3738..4463
                     /locus_tag="VEJ00_05295"
     CDS             3738..4463
                     /locus_tag="VEJ00_05295"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX00609.1"
                     /translation="MRAETRRQLKQDAFSRVTLEAAEKTAHWTVEHRSTLIISGVAVA
                     VVLAAVIGGWFYLSAQDEKASLELSQAVRTLSTPLRPAGTPQQPDYPTFQSSQERSEA
                     AKKQFQAIADRYPHTRTADMAHYFLGVTAENLSDNATAERNFKEVASVGNRDLSAVAK
                     MALASLYGQTNRTKEAAALYQELINKPTQSVSKVTAQLQLAELYQNSNQPLDAKRIYE
                     QIKRENPSSEAGQLATQKLAELK"
     gene            4656..>6201
                     /locus_tag="VEJ00_05300"
     CDS             4656..>6201
                     /locus_tag="VEJ00_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00610.1"
                     /translation="MTCRRCVFPIAMCLMGVSILGFSQDDSSASSGQSLGDVARRLRK
                     DTTDEVRMTDADTKKLFESVDKIFTFASEDTDMPKHAPVKRQLVSKADVEKYASGRLA
                     KEEFTQRFAQGELSMKKLGFLPREFNLREFLVKSTGQQIAGYYDDETKTISLLNWVPF
                     DRQEPILAHELTHALQDQNYNLKKWMQAALKPGGDGANAASGNDDGATARKAVVEGQA
                     MVVYVDYVLKPLGRNLADTPGLIYQMEEPAVKAVADSQMLHDAPMILRESGTFAYNQG
                     LIFEGELLHKGGKKMAFAGVFARPPKNSHEVLQPDAYINGEKLPPVAIPDLKQLLGND
                     YDVYDSGGIGQLDVRALLKQFGERKIADDLSSAWQGGAYVTFHRKDKPAPAATTADLA
                     LLYVSRWKTPQAAEHFARFYTGAVAQRYQSATAEPAPPCAGSSCPVASAQIATEEGPV
                     IVEQWADNSVVVSESFDSSMAAKIQSAMRGGSAGAHAANYSQEEIGLGLFDVPAFRAF
                     AEGIGAK"
BASE COUNT         1203 a         1870 c         1954 g         1174 t
ORIGIN      
        1 tcttcatctt tgggcttcca gttcggcggc tttaccagca ggctcttgcg gaactcgaag
       61 gggtcttgcc cggccgcagc ggcaagctcg tcgatgaaac tttcgactgc gaatccgttg
      121 aaggaattct ccaccgagcg ccaccaggcc cggggtacag cgctggcgac cggactgtat
      181 tccaggcgaa tgttggggat ggcatagggg atctgtttcg ccccgccgat ctcgtcgtcc
      241 tctggaggct tcttcggatc ccaatatccc tgaatcgccg tagagaccag agtgtggaac
      301 cacgcggtcg ggcgcccctg ggcatcgatc gcgccacgaa agcgatggca cgatgccggc
      361 cggtagaagt cgtgagtcat gtcgtcctcg cgcgaccaca cgagttgcac gggcttgccg
      421 acgactttgg cgatctgtgc tccttcggcg gggaaatccg ccatgtagcg gcgcccgaat
      481 ccgccgccca tgaacgtggt atgcacagtc actttctgcg gcgtgatgcc cagcaccttg
      541 gccaccgttc cctgcaccca atcgggcgat tgcgtgggtg cccacacttc ggcttcgccg
      601 ccacgcgcat gcaccgtgat gttcataggt tccatggtcg cgtgcgccag aaagggaagc
      661 tcgtagagcg cttccaccgg cttggcggcg tgagcgagtg cagcatcgac gtcgccatcg
      721 ttgcggacgc gcttgcccgg tttctgagcg gctgccagca gggacttgcg caggctatcg
      781 ttcgactccg aaaccgccgg gccgtggtcc caggtgatgt cgagcgcgtc gcggcctttc
      841 atcgctgccc aggtggaatc agcgatgacc gcaactccgc cagcggtgaa catgtcagcg
      901 cccatcgccg gaatctcgaa aacgtcgcgc acgccggaga ccgctttcgc cttggcggtc
      961 tcgaagtgcg cggctttgcc gccgaaggtc gggcaccgcg caacgaccgc aaacagcatg
     1021 tcgggcacac gcacatcgag gccgaactgt gccctgccgt tcactttaga gggaatgtca
     1081 acccgcggcg tggcgtggcc gatgagttcg aacttcgatg gatctttgag cgggactttc
     1141 tcgggatcgg gacggggcaa ctttgctgca gcgtccacaa gctcaccgta gcccgcctgc
     1201 cgggatgatg ccgcatgcag aaccgcacct tttctggcct ggcactggcc ccgcggcacg
     1261 ttccacttgg ctgcggcggc ggtgatcatc agttcgcgaa ccgtcgcccc ggcctgtcgg
     1321 agcggcgtga agttctccct ggtgctgctg cttccgcccg tgccgaggtg gggataaata
     1381 tctggacgcg tctcggcctg ctcgacgctg acggagttga aatcgacctc ggcctcttcc
     1441 gcgagaacca tggcgagtcc ggtcttgatt ccctggccca tttccgactt ggccacgatc
     1501 agcgaaatct ggccgttcgg cttgacatga atccaggcat caaagggatt gggcgcgggc
     1561 ttcttgcgcg ccttcgcgtt ttcctgcgcg agggcaaaag gcacggagag gcgcacgccg
     1621 atgacaagtc ctgttcctgc agcggctcct cgaacgacaa attcgcggcg gctgatcgtc
     1681 atggctatgc tccttgtgca gcgctcttgc tggcgcgctg gatagcgcgg cgaatccgct
     1741 gatacatgcc gcagcggcac aggttgccga ccatggcgtc gtcaatctgg tcgtcggatg
     1801 ggtgaggcgt tttcgcgagc agtgcggccg cggacatgat ctggccgcac tggcagtatc
     1861 cgcactgtgg cacctgttcg gcaatccacg ccagttgcac gggatgcgaa ttgtccgtcg
     1921 acaggccttc gatcgtcgtg atcttcttct tcccaacgtc cgagacggcg atctgacagg
     1981 agcgcaccgc ctcgccatcg agatgaaccg tgcaggcgcc gcacaggccc aggccgcagc
     2041 cgtacttggt gcccgtcatg ttcagtttgt cgcgcagcac ccacaacagc ggggtgtcgg
     2101 gggaaacatc agtgtcatgt ggactgttgt tgatggtcag atgaaacgca gccataagaa
     2161 ttctccggca gggaagaatg tgaagtgaca cgaggctaac gcaacaggct ttgcctgagc
     2221 aaatcggaat tcgcaggata aaagtgcggt ctgggttaga gacgggtgtc agaagcccgg
     2281 ctgttcccag gtgcggtttg ccagagtctg ctggacgcgc agttggtacg gttgtccggg
     2341 ctgccagttg ccggaaaagt gcgccccgat gcaggcccag aaaacatagt cccagccgtg
     2401 ctgggcggcg atctggtcga cggtctggcc caggtagagc cactggccgc catcgtagaa
     2461 gtcgccgtcg tagcaggagc ggatgtgggc ggcgtaccag tcgacgttca gcgccgtcga
     2521 ctgcgacgtg gcgggatagc ctccatgaat tacggtgccg gaacagtctt cgttcttgat
     2581 ctgcattagg ccgtaggaac cctcgccgac cggcccgcac acgtcgccga tcgtgctcat
     2641 gtgccagtag gattcgatga cgccatcggc gcggatggtg tcctcgtcga tgccccactt
     2701 gcatgcggcc cactggatga tctcagtggt cgtgcctgta aagttgccgg tgacctgggc
     2761 gcgcttgtcg cgccacttcg tccagtagtt ttcaaccgac cagttgtagg gcggagacag
     2821 gacagtgtga ttggcctggt cgttgtcggg ccgcggttca tacgagttgg gcgttaccgc
     2881 cgaagcgcaa taggcatcgg agcggggaag aaacgtcgag tgggcaggct ctgtaccgaa
     2941 gtagccgggt ttggtgggag gagtcttcgg aggctgattg gcggtcgttg gctggcctcc
     3001 gcaacccagc agaatggcta gcagcgccag tgcgccggcg gccgatcgtg ctcatgtgcc
     3061 agtaggattc gatgacggcg tcggcgcgga tggtgtcctc atcgatgccc cacttgcacg
     3121 cggcccactg gatgatctcg gtggtcgtgc ctgtacagtt gccggtgacc tgggcgcgct
     3181 tgtcgcgcca cttcgtccag tagttttcga ccgaccagtt gtagggcgga gacaggaccg
     3241 tgtggttcgc ctggtcgttg tcaggccgcg gttcgtacga gttcggggtc acggccgagg
     3301 cgcagtaggc atcggaacgg ggaaggaacg tcgcatgaga aggctctgtg ccgaagtagc
     3361 caggtttggt gggaggtgtc ttgggaggct gattggaggc ggttggctgg cctccgcaac
     3421 caagcagaat ggctagcagc gccagtgcgc cggcggccga tcgtgccttc gggcggctgc
     3481 accggagcga catttgatga ataaccgcaa tagccacaac gccctccgtt aacgattagc
     3541 gtatcgcggg tgtaacgaga gacatatgtt ccggaagggc actggggagc aggactggtt
     3601 gcacgaacgg cacgggcgga ggtcgcaagg tgtgattgat cagaaggtca gtgcctggca
     3661 cgctttttga cgctccgttt tgtcgcctgt taaactgcta gttcgtccaa ccgcagttac
     3721 cacgagaagg gaagtctgtg cgcgcagaaa cccgccgaca actgaaacag gatgccttca
     3781 gccgcgtcac cctcgaagct gctgagaaaa cagcccattg gacggtggag caccgctcga
     3841 ccctgatcat ttccggagtt gcggttgcgg tcgttctggc cgccgtgatc ggcggctggt
     3901 tttatctgag cgcgcaggac gaaaaggcca gcctggagct ttcccaggcg gttcgcaccc
     3961 tcagcacccc tttgcgtccc gcgggcacac cgcagcagcc ggattacccc acctttcagt
     4021 cctcacagga gcgctcggag gcggcgaaga agcagttcca ggccatcgca gacaggtatc
     4081 cgcacacgcg cacggccgac atggctcatt actttctggg agtgacagcc gagaatcttt
     4141 cggacaacgc caccgccgag cgcaatttca aggaggtggc ttcggtcggc aaccgcgacc
     4201 tgtccgcagt ggccaagatg gcgctcgcct cgctctacgg ccagaccaac cgcacgaaag
     4261 aagccgccgc cctctatcag gaactgatca acaagcccac gcaatcggtc agcaaagtga
     4321 ccgcacaact gcagttggcc gaactttacc agaactccaa ccaacccctc gatgccaagc
     4381 gcatctacga acagatcaag cgcgagaacc ccagcagcga ggcaggacag ctggctacac
     4441 agaaactggc agagctgaag taagcagtgg tgagtgatca gtggccagca aatcccactc
     4501 ggaatcgatt tccccgctga gcactcatca ctgataactg gtcactgact tcgattacct
     4561 tcgtaagccc aagttctacc ttttgccgtg atcctcggcc agccctcaaa gacaatcgat
     4621 agaagcgcat ttttgtcgtt gaatgaggct cgacaatgac ctgccgccgc tgcgtcttcc
     4681 cgattgcaat gtgtctcatg ggggtgtcca tccttggctt cagccaggac gattcttccg
     4741 cctcctccgg acaaagcctc ggcgacgtcg cccgcaggct gcgcaaagac accacggacg
     4801 aagtccgcat gaccgacgcc gataccaaga agctgttcga gtcggtggac aagatcttca
     4861 ctttcgcctc tgaagacacc gacatgccca agcatgcgcc ggtgaagcga cagctggtca
     4921 gcaaggccga cgtggagaaa tatgccagcg gccgcctggc caaggaagaa ttcacgcagc
     4981 gttttgccca gggtgagttg agcatgaaga agctcgggtt cctgccgcgc gagttcaacc
     5041 tgcgcgaatt tctggtgaag tcgacagggc agcagatcgc cggctactac gacgatgaaa
     5101 ccaagaccat ctcgctgctg aactgggtgc cgttcgaccg gcaggaaccg atcctggccc
     5161 acgaactcac gcatgcgctg caggatcaga attacaacct gaaaaagtgg atgcaggccg
     5221 cgctcaagcc cgggggtgat ggcgccaacg ccgccagcgg caacgacgat ggcgcaaccg
     5281 cacgcaaggc ggtggtcgag ggccaggcca tggtcgtcta tgtcgactat gtgctgaagc
     5341 ctctggggcg caacctcgcc gacacgccgg gcctgatcta tcagatggaa gagcccgcgg
     5401 tgaaggccgt ggccgactca caaatgctac acgatgcgcc catgatcctg cgcgagtcgg
     5461 gaacgtttgc ctacaaccag ggcctgatct tcgaaggcga gctgttgcac aagggcggca
     5521 agaagatggc atttgccggg gtcttcgcgc gcccgcctaa aaattcacat gaggtgctgc
     5581 agcccgatgc ctatatcaac ggcgaaaagc tgccgccggt cgcgatcccc gatttaaagc
     5641 agctactggg caacgactac gacgtctacg actcgggcgg catcggccag ctcgatgtgc
     5701 gcgccctgtt gaagcagttc ggcgagcgga agattgcgga cgatctctct tccgcctggc
     5761 agggcggcgc ctatgtgacc ttccatcgta aagacaagcc cgctccggca gcgactacgg
     5821 ccgacctggc tcttctctac gtctcgcgct ggaagacgcc ccaggccgcc gaacactttg
     5881 cccgcttcta caccggagcg gttgcgcagc gctatcaaag cgcgacagcc gaaccagcgc
     5941 cgccgtgtgc ggggtctagt tgtccggtag ccagcgccca gatcgcgacc gaggaaggcc
     6001 cggtgatcgt ggagcagtgg gcagacaaca gcgtggtggt ctccgagagc tttgacagca
     6061 gcatggccgc caagatccag agcgcgatgc gcggcggcag cgcaggcgcg cacgctgcaa
     6121 actattccca ggaagaaatc gggctaggcc tcttcgacgt cccggccttc cgcgcgtttg
     6181 ccgaaggcat cggcgcgaag a
//
LOCUS       DASPUL010000222         4258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1954704, whole genome shotgun sequence.
ACCESSION   DASPUL010000222 DASPUL010000000
VERSION     DASPUL010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1954704"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VEJ00_05305"
     CDS             <1..327
                     /locus_tag="VEJ00_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00611.1"
                     /translation="VESLSHYIQKHDEYSNWEARVLLQGEQGGTELPPAVFGTQAQRR
                     RWLKKKLYALPGSPVLLFFYRYFLRLGFLDGIPGLIYCGFQAVQMFHTKAKIYELKKS
                     MQHRQG"
     gene            343..2334
                     /gene="asnB"
                     /locus_tag="VEJ00_05310"
     CDS             343..2334
                     /gene="asnB"
                     /locus_tag="VEJ00_05310"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXX00612.1"
                     /translation="MCGISGLVNCGDRETLARMTQVQAHRGPDDSGLWERRFPDGSYV
                     GLGSRRLAILDLSADGHMPMCNEDRTIWITYNGEIYNFADLRRELEGKGHRFLSRTDT
                     EVILHLYEQDGPDCVKRLNGMFAFAICDLRSATPILFIARDHFGVKPFYYFHDGKRFA
                     FASEIKGLLQVEGIPAELDVESLHQYLTFLWVPDPKTMFRRIFKLPAGHYATLRQGEF
                     RLTQYWDLTFPAADQTYARPEADLAGEVRERFRRSVQAQMVSDVPIGAFLSAGLDSSS
                     IVAMMQQAATQPVRTYTITFPPKYRVGETTLDDPEVATRLARHMGCENQRIVVEPDVT
                     ELLPRLVWHMDEPTADPAIITAYLVCREARKQATVLLSGVGGDELFAGYRKHVAHYWA
                     QAYRRMPSGVRRTAEAALGALPSLRGTALKGRLRLARKMARSATLGPVDRFITNCTYL
                     DEAQKSRIYTAEFEAKASGSDPARQHHAAFERVCEADFLHQMLYLDTKIFMTTLNLTY
                     NDKMSMASSVEVRVPFLDRELAEFVAWNVPPGLKLKGFLRPTTKYIFRRAMKDILPNE
                     VLRQPKAGFAAPIDYWLAHDLREMVDDLLSPDQLRKRGIFDPEVLRKYVSEHRSGRQD
                     WSMQVWQFLTLELWMQTFLDGGAQRFESESLPPRQVATA"
     gene            2400..4136
                     /locus_tag="VEJ00_05315"
     CDS             2400..4136
                     /locus_tag="VEJ00_05315"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HXX00613.1"
                     /translation="MLILGLNMFHADASAAIVIDGEVKFAIAEERLNRHKHFGGFPAL
                     AVKACLDAVGAKITDVDHVGVGQDSDANLRKKVQYALANPAKILNFIRLRQRKEAMRD
                     VRALLSKALEVDSAKLRFQEHHLEHHIAHIASAYYCSPWEKAAGFSYDGSGDFVSTMM
                     ARCEGNEIEVLDRVFLPHSLGSLYTMICEFIGYTKYGDEGKVMGLAPYGKDTYCPEIN
                     KIVALKNGAFQLDLGYFKPLGSNQGMQVLPDGTVRLARHYSARVEKLFGEPRQPHTEI
                     TRRDMDLAFALQHRFEEVFFHLLNELHRRVPLDDIAMAGGCALNSVANGKLFDRTPFR
                     RTYIQPAAGDEGLAIGAALHTYHSVLKQPRRHELRNAYLGPEFSDSYIQSALHRAGLK
                     SRKLERGPLLDAVAEQIAAGNVVGWFQGRMEWGPRALGNRSIVAHPGLPNMKDVLNAR
                     IKHREWFRPFAPSILADKQHEYFEHDHPSPFMLHVYKIRPEKRKALCAVNHVDDTGRL
                     QTVTREENPLYYDLISTFARKTGIPVILNTSFNENEPIVCTPEEAIDCFQRTRMDVLA
                     IGPFLVVKEAKS"
BASE COUNT          878 a         1307 c         1211 g          862 t
ORIGIN      
        1 gtggaatcgt tgtcgcacta tatccagaag cacgatgaat actcgaactg ggaagcgcgt
       61 gtcttgctcc agggcgagca gggcggaaca gagttacctc ccgcagtctt cggtactcag
      121 gcccagcgcc gccgctggct caagaagaag ctgtatgctc tacccgggtc gcctgttttg
      181 ctgttctttt atcgctattt tctgcggcta ggatttctgg atggtattcc cggcctcatc
      241 tactgtggat ttcaggcggt gcagatgttc cacaccaaag ccaagatcta cgaattgaag
      301 aaatccatgc agcacaggca aggctgacaa gggcgacctc aaatgtgcgg aatcagcggt
      361 ctggtgaatt gcggggatcg ggagactctg gcgcggatga cgcaggtcca ggcgcatcgc
      421 gggcccgatg actccggcct gtgggagcgg agattcccgg acggttccta cgtcggcctc
      481 ggcagccgac gcctcgccat tctcgacctg tcggctgacg gtcacatgcc aatgtgcaac
      541 gaagatcgca caatctggat cacctacaac ggtgaaatct acaacttcgc ggaccttcgg
      601 cgcgagctcg agggcaaagg ccaccggttt ctgtcgcgaa ccgacacgga ggtgatcctt
      661 cacctgtacg agcaggacgg tcctgactgc gtaaagcgat tgaatggcat gttcgccttc
      721 gcgatatgcg acctgcgctc ggcgacccca attctgttca tcgcccgcga tcacttcggg
      781 gtcaagcctt tctactattt ccacgatgga aagcgcttcg cctttgcctc ggagatcaag
      841 gggcttctgc aggtggaagg catcccggcg gaactcgatg ttgaatcgct ccaccagtat
      901 ctgaccttcc tgtgggttcc cgatccgaag acgatgttcc gtcgcatttt caagcttcct
      961 gcgggtcact atgcgacgct gcgacagggg gagttcagac ttacacagta ttgggatctg
     1021 acatttcctg ccgccgatca gacctacgca cgacctgagg ccgatctcgc cggcgaggtt
     1081 cgtgagcgtt tccgccgctc ggtgcaggct cagatggtca gcgatgttcc catcggcgcg
     1141 tttctaagcg ccgggctgga ctcttcgagc atcgtcgcca tgatgcaaca ggcagcgacc
     1201 cagccggtcc ggacttacac gatcactttt cccccgaaat atcgcgtcgg cgaaaccacg
     1261 cttgacgacc cggaagtggc cacgcgattg gcgcgccaca tgggatgcga aaaccagcgc
     1321 atcgtcgtcg agcctgatgt gactgaactc ctgccgcggc tggtatggca catggatgag
     1381 cccaccgctg atcctgccat catcaccgcc tatcttgtat gccgggaggc tcgcaaacag
     1441 gcgaccgtgc tgttgtctgg tgtcggtggc gacgaactct tcgctggata tcgcaagcac
     1501 gtcgcgcact actgggcgca agcttatcgc cgcatgccct cgggtgtccg ccgcacggct
     1561 gaagccgctc tgggtgcgct gcccagcctc cgcggcacgg ccttgaaggg gagattgcga
     1621 ctggccagaa agatggcacg cagcgctacg ctcggcccgg tggatcgctt catcacgaac
     1681 tgcacgtacc tcgacgaagc acagaaatcc agaatctaca ccgccgaatt cgaggcaaaa
     1741 gcctccgggt cagatcccgc acgccagcac catgctgcgt tcgagcgcgt gtgcgaggcc
     1801 gatttcctcc accagatgct gtatctcgat accaagatct tcatgacgac cctcaacctc
     1861 acctacaacg acaagatgag catggcgtca tcggttgagg tccgcgtgcc ctttcttgat
     1921 cgcgaactgg ccgagtttgt cgcctggaat gtgcctcccg gtttgaagct caaaggattt
     1981 ctccgtccga ccaccaaata catcttccgg cgggcgatga aggacattct gccgaacgaa
     2041 gtgctgcgac agcctaaggc cggcttcgcc gctcccatcg actattggct cgcgcacgat
     2101 ctcagggaga tggtggacga cttgctttcc ccggaccagc tgcggaagcg tggaatattt
     2161 gatcctgaag tgctcagaaa atatgtcagc gagcatcgca gtggacgcca ggattggtcg
     2221 atgcaggttt ggcagtttct gacgctggag ctctggatgc agacatttct cgatggggga
     2281 gcgcagaggt tcgagtccga atcacttccg cccagacaag tggccacggc atgaagcaag
     2341 gcccaccggg acaaatgacc cgatcccaga ccaaccattg caggagtcgc agctagctca
     2401 tgctcatttt gggcctcaac atgtttcacg ccgacgcctc ggcggcgatc gtcatcgacg
     2461 gcgaagtgaa gtttgcgatc gccgaggagc gattgaaccg ccacaagcac tttggcggct
     2521 ttcccgcgct tgccgtgaaa gcctgtctcg atgcagtcgg cgcaaagatt accgatgttg
     2581 accacgtcgg ggtgggccag gacagcgacg ccaatctcag gaagaaagtc caatacgcgc
     2641 tcgccaatcc cgccaagatt ctgaatttca tccgcctgcg ccagcgcaaa gaagccatgc
     2701 gcgacgtgcg cgcgctgctc tcaaaagcgc tcgaagtcga ttccgcaaag cttcgctttc
     2761 aggaacacca cctcgaacat cacattgccc acatcgccag cgcgtactac tgctcgccct
     2821 gggagaaggc cgcagggttc agctacgacg gttcaggaga cttcgtctcc accatgatgg
     2881 cccgctgtga gggcaatgaa attgaagtgc tcgaccgcgt cttcctgccg cactcgctgg
     2941 gcagcctcta caccatgatc tgcgaattca tcggctacac gaaatatgga gatgaaggca
     3001 aagtcatggg cctcgcgccc tacggcaagg atacctactg cccggagatc aacaagatcg
     3061 tcgccctcaa gaacggcgcc ttccaactcg acctggggta cttcaagccc ctcggaagca
     3121 atcagggaat gcaggtgctg cctgacggta cggtgcgcct ggcgcgccac tattccgccc
     3181 gcgtggaaaa actgttcggc gagccgcgcc agccgcacac cgagatcaca cggcgcgaca
     3241 tggacctggc tttcgccctg cagcatcgct ttgaagaagt gttcttccat ctcctgaacg
     3301 aactgcaccg gcgcgtcccg ctcgacgaca tcgcgatggc cggcggatgc gcgctcaaca
     3361 gcgtcgccaa cggaaagcta ttcgatcgaa cgcctttccg ccgaacctac attcagccgg
     3421 ctgcaggcga tgagggcctg gccatcggcg ctgcgttgca cacttatcac tccgttctga
     3481 agcaaccgcg ccgtcacgaa ctgaggaatg cctatctcgg gccggagttc tccgattcgt
     3541 atattcagtc ggcgctgcac cgtgcgggcc tgaagtctcg caagctggag cgcggcccct
     3601 tgctcgatgc ggtagccgag cagatcgccg ccggcaatgt cgttggctgg tttcaggggc
     3661 gcatggagtg gggcccgcgc gcattgggca atcgctcgat cgtggcccat cccggcctgc
     3721 caaacatgaa agatgttctc aacgcccgca tcaagcaccg ggagtggttt cggccattcg
     3781 ctccgtctat cctcgccgac aagcagcacg agtattttga gcacgatcat ccgtcgcctt
     3841 tcatgcttca cgtctacaaa atccgcccgg agaagcgtaa agcgctatgc gcggtcaatc
     3901 acgtcgatga cacgggccgc ctgcagaccg taacgcggga agagaatccg ctctactacg
     3961 acctgatctc aaccttcgcc cgcaagactg gaattccggt gatcctgaac accagcttca
     4021 atgagaacga gccgatcgtc tgtaccccgg aagaagccat cgactgcttt cagcgcacgc
     4081 gcatggacgt tctcgcgatc gggccctttt tggtggtgaa agaagcgaag tcatgagaga
     4141 agcgggatcc cgtcctccga gccttcaagg atacgatgtc gctccgcctg ttttttgacc
     4201 ggtgggtttc gtcctcagtg ccggagacct caaactgctt ggcgttttgc ctgcgttc
//
LOCUS       DASPUL010000223         4694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_447803, whole genome shotgun sequence.
ACCESSION   DASPUL010000223 DASPUL010000000
VERSION     DASPUL010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447803"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="VEJ00_05320"
     CDS             <1..1276
                     /locus_tag="VEJ00_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXX00614.1"
                     /translation="ARFARACVDAGVKFIGPTAAAMDAMGSKTQARQAMERAGVPIVP
                     GTSHGLGSLEEGQEVAGRIGYPVMLKAAAGGGGKGMRLVHAPQDLKSALEGARSEAER
                     SFGDDEVYIEKAIVNPRHIEMQILADEHGNTVYLGERECSIQRRHQKVVEEAPSPIVD
                     ADMRRRMGEVAVRVAQTAGYTNAGTVEFLVDQQKNFYFLEMNTRLQVEHPVTELITGL
                     DLVHLQIRIASGERLPFTQDQVTIRGHAIECRIYAEDPDNNYFPSPGKITLLLQPSGP
                     GIRRDSGMYEGWTVPMDYDPLLAKLIGYGTDRDQAISRLTRALNEYFVGGIKTNISLF
                     RHILRNPDFLAGKLDTGFLDRMLSRKEDSEVDSEVGAKTEEIAAIAAGLFAALGVAAA
                     GAGEQVPFNGAAGGNSKAPSNWKSVAHREALR"
     gene            1356..1850
                     /locus_tag="VEJ00_05325"
     CDS             1356..1850
                     /locus_tag="VEJ00_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HXX00615.1"
                     /translation="MTYDVTIDGKNYRLELARPDGRWSCRLDGQELEVDAVLARPNVL
                     SLRLGNQAYEVKCERVGGEMHVWVGSARFAAEVRDPRSLRARARAADDHGPRKLTAPM
                     PGKIVRVLASQGAEVEAGMGVLVVEAMKMQNEIKSPKKGTIQKILVSEGAAVNAGDVL
                     AIVE"
     gene            complement(1887..2954)
                     /locus_tag="VEJ00_05330"
     CDS             complement(1887..2954)
                     /locus_tag="VEJ00_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXX00616.1"
                     /translation="MRKHVLLAQAAATLAASILLALLAACSSPAGGDVMATVDGRKIF
                     RSDVDKYYENYQASSAQQAAGGEQATALRLQILNQMIEDEMLMRRAEKLGLLATDEEV
                     DRKLNEIKAPFTQEEFDQRLKDKKITVADFKRDIRRSITREKVLNKEVSSKINITDQD
                     ISDYYNAHKGEFNLIEPQYHLAQIMVTPLPNPQAHNQNDKAQNEADAHKKIQMIANRL
                     DSGDDFATLAMKYSEDPETSGNGGDLGTVPESSLRSTDPLTRDAVMRLKPGQYSAIIS
                     VVNPQTKQEVGYRIVKLVAKEPAGQRELNDPRVQQAIRSQLHDRREQLLKAAYYEVLR
                     DSAKVENFYAKKVLDSNGMEK"
     gene            complement(3045..3974)
                     /locus_tag="VEJ00_05335"
     CDS             complement(3045..3974)
                     /locus_tag="VEJ00_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXX00617.1"
                     /translation="MTIVRRSFVVSLLICLGCIAQSAPSELTRKIERQVRVHYTIPAD
                     VPVVVGAITPSDDLPGYDTVAVTIGGGDTKKQDYKFLLSKDRNTLLRLTKFDLTKDPY
                     ADLMSKINLGGRPSRGAKDAKVVVVNFDDFECPFCSRMHQELFPEIFKEYGDRVSFVY
                     KDYPLSEIHPWAIHAAVDANCLAAQNADAYWDFADYIHANKREVDTEKTPGARFEAID
                     KIAVLQGQKHNADIPRLQACVKAQNDSEVRASMKEGDDIGVSATPTLFINGQKIDGAV
                     PISELRAALDNALREAGQPVPAHSPSAATPASR"
     gene            complement(4052..>4694)
                     /gene="larE"
                     /locus_tag="VEJ00_05340"
     CDS             complement(4052..>4694)
                     /gene="larE"
                     /locus_tag="VEJ00_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HXX00618.1"
                     /translation="LSFAGEQGFAVEVIATAELDRPEYARNDGQRCFHCKDELFTVME
                     QIRAARGFDAIAYGVNVDDQGDFRPGQQAAKLHQVAAPLLDAGLTKQDVRSLARHAGL
                     RIWDKPASACLSSRIEYGRPVTREALDVVERGEEALRALGFRQFRVRHHGDIVRIEIA
                     REELDRALDPAMAAEFTAIFKALGFQFVTLDLEGFRSGSMNSLLPADQLRRTG"
BASE COUNT          887 a         1357 c         1489 g          961 t
ORIGIN      
        1 tgccaggttc gcccgcgcct gcgtggatgc aggggtgaag ttcattggcc cgaccgcggc
       61 ggcgatggat gcgatgggat ccaagactca ggcccgccag gcgatggagc gagccggagt
      121 tcccattgtg cccggtacct cgcacggcct gggctcgctg gaagaaggtc aggaagtggc
      181 cgggcgcatc ggctatccgg tgatgctgaa ggcggcagcc gggggcggtg gcaagggcat
      241 gcggctggtg cacgcccctc aggatctcaa atcggcactc gaaggcgcgc ggagcgaggc
      301 ggagcgctcg tttggcgacg acgaagtcta catcgagaag gcgatcgtca atccgcgtca
      361 catcgaaatg cagattctcg cggacgaaca tggaaatacc gtgtatctgg gtgagcgcga
      421 atgttcgatc cagcggcggc atcaaaaagt ggtggaggag gcaccttctc cgattgtcga
      481 tgccgatatg cggcgccgca tgggcgaagt ggcggtccgc gtggcgcaga ccgccggtta
      541 cacgaacgcc ggcacggtcg aatttctggt ggaccagcag aagaatttct attttctcga
      601 aatgaatacg cggctgcagg tggagcatcc cgtgaccgag ttgatcacgg ggctcgatct
      661 ggtgcatctg cagattcgca tcgcttcggg agagaggctg cccttcacgc aagaccaggt
      721 tacgattcgt ggccatgcga tcgaatgccg tatctatgcc gaagatcccg acaacaacta
      781 cttccccagc ccaggaaaaa tcacgctgct gttgcagcca tccgggccgg gcatccggcg
      841 cgacagcgga atgtacgagg gctggaccgt gcccatggat tacgatccgc tgctggccaa
      901 actgatcggg tatgggacag accgagatca ggccatcagc cggctcacgc gcgccctgaa
      961 tgaatacttc gtgggcggga tcaagaccaa catttcgctg ttccgacaca ttctgcgcaa
     1021 cccggatttc ctggccggca agctggacac gggttttctc gatcgcatgc tgagccggaa
     1081 ggaggactct gaggttgatt ccgaggtcgg ggcgaagact gaagagatcg cggcaatcgc
     1141 cgcaggtctg ttcgccgcgc tcggggtggc cgcagccggg gcgggcgagc aggtaccgtt
     1201 taacggagct gcgggaggga attcgaaagc tccctccaat tggaagagcg tggcgcatcg
     1261 cgaggcgctg cgatgaatcg tccaaaaaat gcagggccga attgtccact tgatcgccgc
     1321 ttagtgtcat tccgccagac aggcgtggtg tactcatgac ctacgacgtg accattgacg
     1381 ggaagaacta ccggctggag ctcgctcgtc ccgacggccg ctggtcgtgc cgtcttgatg
     1441 gccaggaact cgaggtggat gcggtgctgg cgcgccccaa cgtgctctcg cttcgcctcg
     1501 gcaaccaagc ctacgaggtg aagtgcgagc gtgtgggcgg cgagatgcat gtctgggtcg
     1561 gaagcgcccg ctttgccgca gaggttcgcg atccacggtc gctgcgggcc cgcgcccgtg
     1621 ctgcggatga tcacggtccg cggaaactca ctgcgcccat gccgggcaag atcgtgcggg
     1681 ttctggcgag tcagggcgcc gaggtcgagg caggcatggg cgtgctggtc gtcgaagcca
     1741 tgaagatgca gaacgagatc aagtcgccga aaaaaggaac gattcagaaa atcctggtca
     1801 gcgaaggcgc cgccgtgaac gccggagacg tgctggcgat tgtggagtaa cggcttccgg
     1861 ctcccggctt ttgggccgga agctgatcac ttctccatcc cgttgctgtc gagcaccttc
     1921 ttggcgtaga agttctcgac cttggccgaa tcccgcagaa cctcgtaata ggcggccttg
     1981 agtaactgtt cgcggcggtc gtgaagctgc gagcggatcg cctgctgcac tcgcggatca
     2041 ttcaattcgc gctgcccggc tggttctttc gccacgagtt tgacgatccg gtacccaacc
     2101 tcctgcttcg tctggggatt gaccacgctg atgatcgcgc tgtattggcc gggcttcagc
     2161 ctcatcacgg cgtcgcgcgt gagcgggtcc gtgctgcgca gcgacgattc cggtaccgtg
     2221 cccaagtcac cgccgttccc ggatgtttct ggatcctccg agtacttcat cgccagggtg
     2281 gcgaaatcat cgccgctgtc gagacgattg gcgatcatct ggatcttctt gtgcgcgtcg
     2341 gcttcgttct gcgccttgtc attctggttg tgagcctgcg ggttgggcaa aggagtgacc
     2401 atgatctgcg ccaggtgata ctgcggctcg atcagattga actcgccctt gtgggcgttg
     2461 tagtagtcac tgatgtcctg atcggtgatg ttgatcttgg aggagacttc cttgttcaac
     2521 actttctcgc gggtgatgga gcgccggatg tcgcgcttga agtcggccac cgtgatcttc
     2581 ttgtccttca ggcgttggtc aaattcctct tgggtgaaag gagccttgat ttcattcaac
     2641 ttgcggtcga cttcctcgtc ggtggccaga agccccagct tttcggcccg ccgcatgagc
     2701 atctcgtctt cgatcatctg attcagaatc tggagccgta gggcagtggc ttgttcaccg
     2761 cccgccgcct gctgggccga ggaagcctga tagttttcgt agtatttgtc tacatccgag
     2821 cggaaaatct tccggccgtc gacggtggcc atgacatcgc cgcccgccgg ggaactgcat
     2881 gccgcgagca gcgccagcaa gatagaagca gcgagcgtcg cagctgcctg agcaagcagt
     2941 acatgttttc gcaagagttt ttctcccgtt ccagccaaga cccgagctcc ttcaagcaaa
     3001 aagccgccgg ggagcggccg gcagggcctg cctgaaatga ttccttatct ggatgccggc
     3061 gttgcagccg atggcgagtg tgccggcacg ggctgccccg cttcccttaa tgcgttatcc
     3121 agggcggcgc gaagctcgct gattggaact gctccgtcga tcttctgccc gttgatgaac
     3181 agagtcggcg tcgcgctgac cccgatgtcg tcgccctctt tcatggaggc tcgcacctcg
     3241 ctgtcattct gcgctttgac gcaggcctgc agcctgggga tgtctgcgtt atgcttctgg
     3301 ccttgcagca cggctatctt gtcgatcgct tcgaagcgag ccccgggcgt cttctcggtg
     3361 tctacttcac gcttattggc atggatatag tccgcgaaat cccagtaggc gtcggcattc
     3421 tgcgccgcca gacagttcgc gtctaccgca gcgtggattg cccagggatg gatctccgac
     3481 agcgggtagt ccttgtacac gaaactgacc cggtcgccgt attccttgaa tatttccgga
     3541 aacagttcct ggtgcatgcg cgaacagaag gggcactcga agtcatcgaa gttgaccacg
     3601 acgacctttg cgtctttcgc gccccgcgag ggccggccgc cgagattgat cttgctcatc
     3661 aaatcggcgt aagggtcctt ggtcagatcg aacttggtca gccgcagcaa ggtgttgcga
     3721 tctttcgata acagaaattt gtaatcctgt ttcttggtgt cgccgccgcc aatcgtcaca
     3781 gcaaccgtgt catagccggg cagatcgtcg ctcggagtga tggcgccaac tacgacgggc
     3841 acgtcggccg ggatcgtgta gtggacgcga acctgacgtt cgatcttgcg agtcagttcg
     3901 gaaggcgcgg actgagcaat gcaacccagg caaatcagca gcgaaaccac gaatgaacgt
     3961 cgaactatgg tcaacaggaa ccgcctcaac atcgcaagag tgaccctttg attatctcac
     4021 aggggcgcgt accagccgcc agcagctggt ctcagcccgt gcggcggagt tgatcggcgg
     4081 ggagcagcga attcatcgat cccgagcgaa atccttccag atcgagagtg acgaactgga
     4141 agcccagggc cttgaatatc gcagtaaact cggctgccat ggcgggatcc agcgcccggt
     4201 ccagttcctc ccgcgcaatc tcgatgcgaa cgatatcgcc gtgatgccgc acccggaatt
     4261 ggcggaagcc cagcgcgcga agggcctctt ccccgcgttc gacgacgtcg agcgcctcgc
     4321 gtgtcactgg acgcccgtac tcgatgcggg atgaaaggca ggcggacgcg ggcttgtccc
     4381 agatgcgaag gccggcatgg cgcgcaagcg agcggacgtc ctgtttggta agtccagcgt
     4441 cgagtagcgg ggcagccact tgatgcagtt tcgctgcttg ttgtcccgga cggaagtctc
     4501 cctgatcatc cacgttcacg ccgtacgcaa tcgcgtcgaa gccgcgggcg gcgcgaattt
     4561 gttccatgac ggtgaaaagc tcgtccttgc aatgaaagca gcgctggccg tcattgcgcg
     4621 catattccgg ccggtcgagc tcggcggtgg cgatgacttc gacggcgaat ccctgctcgc
     4681 cagcaaaaga gagg
//
LOCUS       DASPUL010000224         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_447805, whole genome shotgun sequence.
ACCESSION   DASPUL010000224 DASPUL010000000
VERSION     DASPUL010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447805"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1033)
                     /locus_tag="VEJ00_05345"
     CDS             complement(167..1033)
                     /locus_tag="VEJ00_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00619.1"
                     /translation="MGLSLSLPTNVAGRTGVYLSDTDQIIARANEELQLLDERKGDSQ
                     EGVWRILTPCALRWLIEQSPSRHTLLLHTAEADPVMVIRLSQQPAVQQCREAPEFTKS
                     TEDKRQNWPTSITDEFAYFLGGEKSYRWPLCDYGHRVEIPEHLHGRWAVLNDKTFASA
                     TYNARKDQWRLKVISSEGQTKFEPDMVKHESASSFWVPIRSSERGDRIAVDIETRQGG
                     NRTLDISSHVTARRIAVYDIEAGKELGSVPVNPEHHYRFEFDLGPDGHRLAILEDEMV
                     RVVDLGGIAPRN"
     gene            complement(1327..>2602)
                     /locus_tag="VEJ00_05350"
     CDS             complement(1327..>2602)
                     /locus_tag="VEJ00_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXX00620.1"
                     /translation="ARFARACVDAGVKFIGPTAAAMDAMGSKTQARQAMERAGVPIVP
                     GTSRGLGSLEEAQEVAGRIGYPVMLKAAAGGGGKGMRLVQTPQDLRSALEGARSEAER
                     SFGDGEVYIEKAIVNPRHIEMQILADEHGNIVYLGERECSIQRRHQKVVEEAPSPIVD
                     ADMRRRMGEVAVRVAQAAGYTNAGTVEFLVDEQKNFYFLEMNTRLQVEHPVTELITGL
                     DLVHLQIHIAAGEKLPFKQDQVTIRGHAIECRIYAEDPDNNYFPSPGKITLLLQPSGP
                     GIRRDSGMYEGWTVPMDYDPLLAKLIGYGTDRNQAISRLTRALNEYFVGGIKTNISLF
                     RRILRNPDFLTGKLDTGFLDRMLSRKEYSEVDSEAGAKTDEIAAIAAGLFTALGVAAA
                     GAGEHVPSNGAAGGNAKASSNWKSAAHREALR"
BASE COUNT          491 a          824 c          725 g          562 t
ORIGIN      
        1 tcctggccat caagacggca cgaccagcgg ccgtcgggac gagcgagctc cagccggtag
       61 ttcttcccgt caatggtcac gtcgtaggtc atgatagtca gtggtcaggg atccgtggcc
      121 agtggtcagt tgccggtcgg actgccgcgt tcctggaaag tggccctcag ttgcgtgggg
      181 caatcccccc taggtcgacc actcttacca tctcgtcttc cagaatcgcc aagcggtgtc
      241 cgtcgggacc gagatcgaac tcgaagcgat aatggtgttc agggtttacg ggaaccgagc
      301 ccagttcctt tcctgcttcg atatcgtaca cagcaatacg gcgcgcagtt acgtggcttg
      361 aaatatccag ggttcggttg ccgccctgtc tggtttcaat atcgacggca attctgtctc
      421 cgcgttcact acttcgaatt ggtacccaaa agctactggc tgattcgtgc tttaccatat
      481 ccggctcaaa cttcgtctga ccctccgatg agatcacctt cagcctccac tgatccttgc
      541 gggcgttata cgtggccgaa gcgaaagtct tgtcattgag gacggcccag cgaccgtgca
      601 aatgctccgg gatctcgaca cgatgtccgt aatcgcaaag gggccagcgg taggatttct
      661 ctccccccaa gaagtaggcg aattcatcag tgatcgaagt gggccagttt tgcctcttgt
      721 cttcggtgga ctttgtgaat tctggtgcct cgcgacactg ctgcaccgcc ggttgctgtg
      781 aaagacggat aaccatcaca ggatcagctt ctgccgtgtg cagaagcagt gtatggcggc
      841 tcggagactg ctcaataagc caccgcaacg cgcatggtgt caggattctc cagacgcctt
      901 cctgggagtc cccttttctt tcatccagta gctgaagctc ctcattcgcc ctggcaatga
      961 tctggtcggt gtcggagaga tacacgccgg ttcttcctgc aacgttagtc ggcagcgaca
     1021 gagacaggcc cactttgcca tcagacgaga accgcaatac gcggaagcta agactgggct
     1081 ggcttcgggt cacgaggccg gcgcgttccc gagccgtgaa tccgactagg actcgaccct
     1141 cgtgatctac tgcgatcgaa ggcccgttgg cttcgcgccg gtccggtggt tcccagccat
     1201 acctccgaag gttcagcacg aaagctgttg ctgtcggaga ctgtgcaggg gcgaactgac
     1261 cggtccaaac gatcatcgcc tccagtatcc aagcgacgct atgcttcaga cgagccgatg
     1321 agggtctcat cgcagcgcct cgcgatgcgc tgcgctcttc cagttggagg aagccttcgc
     1381 attcccccct gcagcgccgt tggacggcac atgctctccc gccccggctg cggccacccc
     1441 gagcgcggtg aacagacctg cggcgattgc cgcgatctca tcagtcttcg ccccggcctc
     1501 ggaatcaacc tctgagtatt ccttccggct cagcatgcga tcgagaaaac ccgtgtccag
     1561 cttgccggtc aggaaatccg ggttgcgcaa aatgcgacgg aacagcgaaa tgttggtctt
     1621 gattccgccc acgaagtatt cattcagggc gcgcgtgagc cggctgatgg cctgatttcg
     1681 gtccgtccca tacccaatca atttcgccag cagcggatcg taatccatgg gcacggtcca
     1741 gccttcgtac attccgctgt cgcgccggat gccgggcccg gatggttgca gcagcagcgt
     1801 gatctttccc gggctgggga agtagttgtt atcgggatct tccgcataga tgcggcactc
     1861 gatcgcatgc ccacgaatcg taacctggtc ttgcttgaac ggcagcttct ctcccgcagc
     1921 gatgtgaatc tgcagatgca ccagatcgag ccccgtgatc aactcggtca cgggatgctc
     1981 cacctgcagc cgcgtattca tttcgagaaa ataaaaattc ttctgctcgt ccaccagaaa
     2041 ttcgaccgtg cccgcgttcg tgtagccggc ggcctgcgcc acgcggaccg ccacttcgcc
     2101 catgcggcgc cgcatatcgg catcgacaat cggcgagggt gcttcctcca ccactttctg
     2161 atgccgtcgc tgaatggaac attcgcgctc acccagatac acgatatttc catgttcgtc
     2221 cgcgagaatc tgcatttcga tgtgacgcgg gttgacgatc gccttctcga tgtaaacttc
     2281 gccgtcgcca aacgagcgct ccgcctcgct ccgcgcgcct tcgagtgcag acctgagatc
     2341 ctgaggggtc tgcaccagcc gcatgccctt gccgccgccc ccggctgccg ccttcagcat
     2401 tacggggtag ccgatgcgcc cagccacttc ctgagcttct tccagcgagc ccaggccgcg
     2461 tgaggtaccg ggcacaatgg gaactccggc tcgctccatc gcctggcggg cctgagtctt
     2521 ggatcccatc gcatccatcg ccgccgcggt cgggccaatg aacttcaccc ctgcatccac
     2581 gcaggcgcgg gcgaacctgg ca
//
LOCUS       DASPUL010000225         5853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1268852, whole genome shotgun sequence.
ACCESSION   DASPUL010000225 DASPUL010000000
VERSION     DASPUL010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268852"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2384)
                     /locus_tag="VEJ00_05355"
     CDS             complement(<1..2384)
                     /locus_tag="VEJ00_05355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXX00621.1"
                     /translation="MIGQTISHYRIVEKLGGGGMGVVYKAQDIKLDRFVALKFLPDDV
                     AQDPQALSRFEREAKAASALNHPNICTIHEIDDEHGQVFIAMEFLDGTTLKHTISGRP
                     LDTDLILSLAIEMADALDAAHAEGIVHRDIKPANIFVTKRGHAKILDFGLAKVLSTAA
                     ATVDGATQTGSLDQQHLTNPGTALGTVAYMSPEQVRGKELDARTDLFSFGAVLYEMAT
                     GNLPFRGETSGVISHAILERAPIPAVRFNPDLPPKLEDIIYKAVEKDRNLRYQSAAEM
                     RADLHRLKRDTETGRAVASGTVAAAQDSGLSASATTPAPAPASAGGGSGSGSAVRSAS
                     TLAAAPSSASASAQVTAIPGAAPGKIWKIAVPAAVVLVALVAAGLYVRSRLATPTKTT
                     PLTERDTVVLADFDNKTGDAVFDDALKQALSVELGQSPFLNVLSDRKVSETLGMMGRP
                     ANQRITADIGRELCLRTGSKAVLGGAISSLGTHYLIDLNAVACNTGDTLAKEQAEATG
                     KEDVLKALGQASSRLRTKLGESLPSVQKFDVPIEATTSSLEALKNYSMGITVGREKGD
                     APSIPFLKRAIELDPNFPMAYAGLAVSYINLQQPSLAAEYAAKAYQLRDRVTEKEKLR
                     ITADYFRTTGEVEKEVQTYELWTANYPRDSVPHNNLGVNASFMGQYDKALAEYQETVR
                     LEPDDSISRSNLGGAYVELDRLDEAKATFDQAFTQKLDGGALRINFYSWAFLRGDTAT
                     MEQQLASAAGKPAVEDAMLSVHSDTEAYYGRFSKARDFSRRAVDSAVRADSKETA"
     gene            complement(2518..3852)
                     /locus_tag="VEJ00_05360"
     CDS             complement(2518..3852)
                     /locus_tag="VEJ00_05360"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HXX00622.1"
                     /translation="MVVSVETSHFQVVCRTVQNLALLILAALVAAPAALAAPGFSTPV
                     RLGYHVGDQWEPAMAADVRGHIYVLYPQYGAVPDCASCAIPTMALLTSNDNGVSWEAS
                     RPLLPFSTGQFDPQVVVDPFDHQTVYASWLQNNKRDIIVARSLDFGRTWSFSVAERSS
                     EDADRPVLAVRGPNVYVGFNHEGEFFVAASHDAGQTFSRVRVNPESAFGWALAGGATV
                     DPAGNIYFGWTSYARHEVPELSVTIFVSRSTDGGRTWNTTLMDVSSASPECGEQSCET
                     GYLGAQITLASDAAGALYALWNAGNTKGEAERIYFSSSTTAGASWSARKSVSGAAAGV
                     EHSFPAVVTGASGDVRIAWMDARASDGAHPSQPLWNTFYRSSTNGGATWGPELQLSSP
                     TRGYDYILPTGFRFPFGDYFSLAIDNEGTTHAVWGEGLNYKSPGSIWYTRGR"
     gene            4021..4497
                     /gene="msrA"
                     /locus_tag="VEJ00_05365"
     CDS             4021..4497
                     /gene="msrA"
                     /locus_tag="VEJ00_05365"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXX00623.1"
                     /translation="MEKATFAAGCFWGVEATFRQIPGVISTRVGYTGGNTENPTYKDV
                     CTDGTGHAEAVEVDFDPAKVRYADLLKVFWENHDPTQVNRQGPDWGTQYRSAIFFHSP
                     EQAAEAEASKEALGKAHRYSKPIATQIVPAVTFYPAEEYHQQYLEKRGLASCHVKS"
     gene            complement(4494..4985)
                     /locus_tag="VEJ00_05370"
     CDS             complement(4494..4985)
                     /locus_tag="VEJ00_05370"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HXX00624.1"
                     /translation="MPPESNKQIVRRFIDELWNGRKLEVADELFAPDCVTHQLRSGEP
                     DSSTPRPPQHMKEHVAGWLVAFPDLQMTAEGMLAEGDMVATSMVFQGTHLGTWSGVPP
                     TGRRVSIRMVVVHRIADGKICEDWALVESLGFLQQLGLAPSTADMIAKAASRGNSAAQ
                     QVS"
     gene            complement(5037..5780)
                     /gene="map"
                     /locus_tag="VEJ00_05375"
     CDS             complement(5037..5780)
                     /gene="map"
                     /locus_tag="VEJ00_05375"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXX00625.1"
                     /translation="MSIETPEELDGMRAAGEVVRRVLEAMQREVRPGVSTAELDEVGE
                     RTMRRHGAQSAPALVYKFPGANCISLNDEAVHGIPGKRELREGDLVKLDVTIEKDGFM
                     ADAAVTVPVGQVSEECQRLVACAERAFEKAMLVARAGFRVSEIGRVIEREVRRSGFAV
                     IRELAGHGIGRTIHEEPRVPNYADPTANQILSEGLVITVEPIIAAGSGRSVVAKDGWA
                     VVTADHRPSAHYEHTLVITKGQPILLTAA"
BASE COUNT         1111 a         1738 c         1800 g         1204 t
ORIGIN      
        1 gcggtttctt tcgagtccgc acgcaccgcc gagtccaccg ctcgccggga gaaatcccgc
       61 gccttgctga acctcccata ataggcttcc gtgtccgagt ggactgacag catggcgtct
      121 tcaaccgcgg gcttgcccgc ggccgaggct agctgctgtt ccatcgttgc ggtgtcaccg
      181 cggagaaaag cccaggagta gaaatttatg cgcagcgctc ctccgtccag cttctgggtg
      241 aatgcctgat cgaacgtcgc cttagcttca tccaaacggt ccaattcgac gtacgcaccg
      301 cccagattgg aacgactaat tgagtcatcc ggctccagtc gcaccgtttc ctgatattcc
      361 gccagagctt tgtcatactg ccccatgaac gaagcgttca caccgaggtt gttgtgcggc
      421 acggagtccc tgggataatt cgcggtccag agttcatagg tttgaacttc cttctccacc
      481 tctccggtgg tgcgaaaata gtcggcggtg atgcgcagct tttctttttc cgtaacccgg
      541 tcccggagct gataggcctt ggccgcatac tccgccgcca gcgacggctg ctggagattg
      601 atgtaggaga cggccaaacc agcataggcc atggggaagt tgggatcgag ttcgatcgca
      661 cgcttcagga acggaatact cggtgcgtcc cctttttccc gtcctacggt gatgcccatg
      721 ctgtagttct tcagcgcttc gagtgatgag gtcgtcgctt caatgggaac gtcgaatttc
      781 tgcaccgaag gcagcgactc tcccagcttc gtgcgcaggc gtgaggaagc ctggcccaga
      841 gccttcagaa cgtcttcttt gccggtcgcc tcggcttgtt ctttcgccag cgtgtcgcca
      901 gtgttgcacg cgaccgcatt cagatcgatg aggtaatgcg tgcccaggct ggagatcgct
      961 cctcccagca cagccttgct gccagtacgc aggcaaagct cgcgtccgat atccgcggta
     1021 atgcgctggt tcgccggacg gcccatcatg cccagcgtct cgctcacctt tctgtcggaa
     1081 agcacgttga gaaacggcga ttgcccgagt tccaccgaaa gcgcctgctt caaagcgtcg
     1141 tcgaagacgg cgtccccagt cttgttgtcg aagtcggcca gcactacggt atctctctcg
     1201 gtcagcgggg tggtcttggt cggagttgca aggcgcgacc gcacatacag tcctgcggct
     1261 actaacgcta ctaagacgac ggcggcaggt acggcaattt tccagatttt gcctggcgcg
     1321 gcgccaggaa tggcggtcac ctgcgccgaa gccgatgccg acgaaggcgc ggctgcgagc
     1381 gtagacgccg accgcaccgc agaaccggaa cccgagccac ctccggcaga tgccggggcc
     1441 ggtgcgggcg tcgttgccga tgcggataag ccgctgtctt gcgctgcggc cacggttcct
     1501 gaagccaccg ctcgccccgt ctccgtgtcg cgcttcaacc ggtgcaggtc ggcgcgcatc
     1561 tccgcagcgc tctgatagcg caggttgcgg tccttctcca ccgccttgta gatgatgtct
     1621 tccagtttcg gcggcaggtc gggattgaaa cgaaccgcgg gaatcggcgc gcgctcgaga
     1681 atcgcgtggg agatcacgcc ggaagtctcg ccccggaacg ggagatttcc ggtcgccatc
     1741 tcatacaaca ccgctccgaa cgaaaacaga tcggtgcgcg catccagttc cttacctctt
     1801 acttgctccg gcgacatgta agcaaccgtg ccgagcgccg tgcccgggtt ggtgaggtgc
     1861 tgttggtcga gcgatccagt ttgcgtggcg ccgtcaaccg tcgcagctgc cgtcgacagg
     1921 accttggcca gcccgaagtc cagaatcttg gcgtgtccgc gtttagtgac aaagatgttt
     1981 gccggtttga tgtcgcggtg gacgattcct tcggcatgtg ccgcatcgag cgcgtcggcc
     2041 atttcaatgg ccagcgacag aatcaggtcc gtgtccagcg gacggccgct gattgtatgc
     2101 ttcagggtcg tgccatccaa aaactccatg gcgatgaaca cttgcccatg ctcgtcgtca
     2161 atttcgtgaa ttgtgcagat attgggatgg ttgagcgcgg aggcagcttt ggcctcgcgc
     2221 tcaaaccggc tcagggcctg cgggtcttgc gccacatcat cgggcagaaa tttcagcgcg
     2281 acgaagcggt cgagcttgat gtcctgcgcc ttgtaaacga ctcccatccc gcccccgccc
     2341 aacttctcga caatgcggta gtgcgaaatg gtctggccga tcattgtgcg acgacatggt
     2401 atgcgtgatg cggtggcaag tcaatgagag ctaacgagtt gacggtgcgc agatctcaat
     2461 agggtgaatc gtcattaaat tccttgatcg agtcgcggga taaatgtcag aggtggacta
     2521 ccggccgcgg gtgtaccaga tcgagccggg agacttgtag ttgaggcctt cgccccagac
     2581 cgcgtgggtg gtgccctcgt tgtcgatggc gagcgagaaa tagtcaccga aggggaagcg
     2641 gaagccggtg ggcaggatgt aatcgtagcc gcgggtcggg ctggagagct gtaattccgg
     2701 gccccaggta gcgccgccgt tggtcgaaga gcggtagaag gtgttccaca acggttggct
     2761 gggatgggcg ccgtccgaag ctctggcatc catccaggcg atgcgcacgt cacccgaggc
     2821 tccggtcaca acagccggga agctatgctc tacgccagct gccgctcccg agacgctttt
     2881 gcgcgccgac cagctcgctc ctgcggtagt ggatgacgag aagtagattc gctcggcttc
     2941 acctttggtg ttgccggcat tccacagtgc gtagagggct cctgcagcgt cggaggccag
     3001 tgtgatctgc gctcccagat agccggtctc gcagctttgc tcgccgcact ccggagacgc
     3061 gcttgaaaca tccatcagcg tcgtattcca ggtacgtcct ccgtcggtcg agcggctcac
     3121 gaaaatggtc accgacaact cgggcacttc gtggcgtgcg tatgaagtcc agccgaagta
     3181 gatgtttccc gccgggtcga cggttgcgcc gccagccagc gcccacccga aagcagactc
     3241 gggattcaca cgcactcgcg agaaggtctg tcccgcatcg tgggaagcgg caacgaaaaa
     3301 ttcgccttcg tgattgaatc ctacgtagac gttaggtccg cgaaccgcca gcacaggacg
     3361 gtcggcgtct tccgaactac gctcggccac cgagaacgac caggtgcggc cgaaatcgag
     3421 cgaacgggcg acaatgatgt cgcgcttgtt gttctgcagc caggaggcat aaaccgtctg
     3481 atgatcgaag ggatcgacca caacttgcgg atcaaactgg ccggtggaga agggaagcag
     3541 tggtcgcgac gcctcccagg aaacgccgtt gtcgttgctt gtcaggagcg ccattgtagg
     3601 aatagcgcaa ctggcgcagt cgggtacggc gccgtactgc ggatagagaa cgtagatgtg
     3661 cccccgaacg tcggccgcca tggcgggctc ccattggtcg ccgacgtgat atccgagtcg
     3721 gaccggggtc gagaatccgg gcgccgccaa cgcagccggt gctgccacaa gcgctgccag
     3781 gatcagaagt gcaagattct gcacagtcct gcaaacgacc tggaaatggc tggtttccac
     3841 agacactacc acctaatccg gagggggacc gcccttgact ttatctgcgt tgcagagcgg
     3901 gcgtcaattg cccataggga taatatgcgg gggatttgca agggcgtgac ttttgcctga
     3961 ctttgtcatc taatactggg tctgaagtac tacgcaatca tcgggttaag ggggatctag
     4021 atggaaaagg ccacgtttgc agcaggctgt ttctggggag tggaagctac gttccggcag
     4081 attccggggg tgatctcgac ccgcgtgggt tataccggcg gcaacacgga gaaccccacg
     4141 tacaaggacg tttgcaccga cgggaccggc cacgccgagg cggtagaagt cgatttcgac
     4201 cctgccaaag tgcgctacgc cgacctgctc aaagtcttct gggagaacca tgatcccaca
     4261 caggtaaacc ggcaggggcc ggactggggt acgcagtatc gctcggcgat cttctttcat
     4321 tcgcccgagc aggccgccga ggccgaggct tcgaaggagg cgctcgggaa ggcgcaccgc
     4381 tattccaagc ccattgcgac gcagattgtt ccggcggtga cgttctatcc cgcggaagag
     4441 taccaccagc agtatctgga gaagcgcggg ctggccagtt gtcatgtcaa aagttagctg
     4501 acctgctggg ccgcactatt tccgcgcgaa gccgccttgg cgatcatgtc ggcggtggat
     4561 ggcgcaagtc ccagttgctg caggaagccc aggctttcca ccagcgccca gtcttcgcag
     4621 atttttccgt ctgcgatacg gtgaacgacc accatgcgaa tgctgacacg cctcccggtt
     4681 ggagggacgc cagaccatgt gcctagatgt gtcccttgga agaccatgct tgtagccacc
     4741 atatcgcctt cggccaacat gccttcggcc gtcatctgca agtcggggaa ggcgacgagc
     4801 catccggcta catgttcttt catgtgctgc gggggacgcg gggtagacga gtccggctcg
     4861 cctgaccgca gttgatgggt aacgcagtct ggggcaaaca actcatccgc gacttccagc
     4921 ttgcgtccgt tccaaagctc atcgataaag cgtcggacaa tttgtttatt ggattccggc
     4981 ggcattcggc ttcccgtggg tatcttcctt ttctaagagt actgtttttt cggagcttat
     5041 gccgccgtca gcagaatcgg ctggcccttg gtgatgacca gcgtgtgctc gtagtgggcg
     5101 gaggggcgat ggtcggccgt gacgacagcc catccatctt ttgcgacaac ggatctgccg
     5161 gagccggcag cgatgatggg ttcgacggtt atgaccaggc cttcggaaag gatctgattc
     5221 gcggttgggt cggcgtagtt cgggacgcgc ggctcttcgt ggatggtgcg tccgatgcca
     5281 tgcccagcca gttcgcgaat gacggcaaag ccgctgcggc gcacttcgcg ctcgatcacc
     5341 cggccgatct ctgaaacacg aaagccggcc cgggccacca gcatcgcctt ctcaaaggcg
     5401 cgttcggcac aagccaccag gcgctggcat tcttctgaga cctggcccac aggaacggtt
     5461 acggcggcgt ccgccatgaa tccatctttc tcgatcgtta cgtcgagttt taccaagtcg
     5521 ccttcgcgca actcacgttt gccgggaatg ccgtgtaccg cttcgtcatt gaggctgatg
     5581 cagttcgctc cgggaaactt gtagaccagt gccggagccg attgcgcccc gtgccggcgc
     5641 atggttcttt cgcccacctc gtccagttcg gcggtgctga cgccgggccg aacctcgcgc
     5701 tgcatggctt cgagcacccg gcgcacgact tcgccggccg ctcgcattcc gtccagttct
     5761 tccggagttt caatggacat gaatgcctct catgaaaagg tctcaggctt ccggggcctt
     5821 cgccggcagg cgtgggccga agaaggcgat gag
//
LOCUS       DASPUL010000226        72762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_997524, whole genome shotgun sequence.
ACCESSION   DASPUL010000226 DASPUL010000000
VERSION     DASPUL010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 72762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72762)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72762
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997524"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /gene="secE"
                     /locus_tag="VEJ00_05380"
     CDS             <1..322
                     /gene="secE"
                     /locus_tag="VEJ00_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HXX00626.1"
                     /translation="ASAGEARMAEALKMDEKEIRGGNGGGAGSGLTTGLAKFGEYPRR
                     LRTFLHDVRVEMKQVNWPSRQDVWSTTIVVTVTVAFFGIYFWITDGIFGRLYILVNNY
                     FHHH"
     gene            336..869
                     /gene="nusG"
                     /locus_tag="VEJ00_05385"
     CDS             336..869
                     /gene="nusG"
                     /locus_tag="VEJ00_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HXX00627.1"
                     /translation="MAKQWYIVHTYSGFEKKVKESLEGRVQAFGLQDKIGQVIIPTES
                     VVEVRGGKKVISPRMCYPGYVLVEMDMDDYTWHVVRSTPKVTGFVGSGQYPSPLTDEE
                     VQNILTRATTTAEKPKPKLSFERGEKVRIGEGPFANFTGVVEEVNSDRSTLKVSVTIF
                     GRSTPVELDFAQVEKVA"
     gene            872..1048
                     /locus_tag="VEJ00_05390"
     CDS             872..1048
                     /locus_tag="VEJ00_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00628.1"
                     /translation="MTSFQPGDRVRINDGVFMHFIGVVTEVNVERKMLKASVTVFDRP
                     LAIEFRFSQVKKAA"
     gene            1062..1490
                     /gene="rplK"
                     /locus_tag="VEJ00_05395"
     CDS             1062..1490
                     /gene="rplK"
                     /locus_tag="VEJ00_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HXX00629.1"
                     /translation="MAKKVQAMVKLQLPAAKATPAPPVGTALGPHGLNIMDFCKQFNA
                     KTSKEPDGMIIPVLVTVYSDRTFTFILKTPPASELLKRAAGIVKGSPEPNRNKVAKVT
                     KKQVQEIAKTKLPDLNTTNLEAAMRSVMGTARNMGIEVTD"
     gene            1502..2224
                     /gene="rplA"
                     /locus_tag="VEJ00_05400"
     CDS             1502..2224
                     /gene="rplA"
                     /locus_tag="VEJ00_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HXX00630.1"
                     /translation="MGRSAKVGGKNIDRARKLVEARPYALEEAAELLRKAHFVKFDET
                     VELVLNLGVDPKQSDQMVRGTVVLPHGLGKSKRVLVIASGEKVREAQEAGADFVGGED
                     MVQKITEGWTDYEAVIATPDMMKSAGKLGKVLGPRGLMPNPKTGTVTFEVARAVKEIK
                     AGKVEFRLDKTGLIHSPVGKISFPAKNLAENAQALLGAVVKAKPAAAKGKYIKKITLT
                     STMGPGIPINIEEAEAATAVAA"
     gene            2251..2823
                     /gene="rplJ"
                     /locus_tag="VEJ00_05405"
     CDS             2251..2823
                     /gene="rplJ"
                     /locus_tag="VEJ00_05405"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HXX00631.1"
                     /translation="MKKKAEKKTQAEELRAQLATVSTVILSTFQGITVEDDTRLRRAV
                     EAAGGHYEVVKNTIAERAAAGTPTEGLLKNLKGTNSIAYTKTDPVSLAKVLTKIAKDV
                     PAFQFRAGWVEGRVISIQEIGQLATLPSKEELVSKIMFMLNAPAQRVATALAALPRNM
                     AVVTSEAVKANKFGGGGAAPEEAPAPQPAQ"
     gene            2874..3263
                     /gene="rplL"
                     /locus_tag="VEJ00_05410"
     CDS             2874..3263
                     /gene="rplL"
                     /locus_tag="VEJ00_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HXX00632.1"
                     /translation="MASKVETILEEIKGLTLLEASELVKKMEEAFGVSAAAAAPVVMA
                     GGAAAGAAAGAPAEEKTEFTVVLSEVGANKINVIKAVREVTSLGLKEAKDLVDGAPKT
                     VKEGVNKDEAATIKKKFEEAGAKVEIK"
     gene            3421..7866
                     /gene="rpoB"
                     /locus_tag="VEJ00_05415"
     CDS             3421..7866
                     /gene="rpoB"
                     /locus_tag="VEJ00_05415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HXX00633.1"
                     /translation="MTQRNGNRVVRERERLDFAKIKTTVQIPNLIDVQKKSYQRFLQM
                     DLLPSEREDSGLQSVFTSVFPIQDFRGLSQLDFVDYAIGNWECKCGHLRGLHHLRATC
                     RNCGASVVTNPYKTGDVICAKCGTFNKNTPTFCNKCGDPVTLQLKYDVNECQERGMTY
                     SAPLKVTIRLTIYDKDPDTGAKSIRDIKEQEVFFGDIPLMTENGTFIINGTERVIVSQ
                     LHRSPGVFFETANNRTYFLGKIIPYRGSWVEFEYDTKNVLYVRIDRKRKFLGSIFLRA
                     LGMKSNEDILRTFYQVERINLREGDLFWNVSEGLIDRKLSKEIKHPKSNEVLVGAHKR
                     ISESLYKEMVKARVNQVEVGIADLEGAVTVSELVNRETGEVLLEANKPLTAEMWATIA
                     EAGITEVDVCFPERDDVGIIVSRTLEKDTIHTPKEALIEIYRKLRPGDPPTLETATAL
                     FNGMFFDARKYDFSKVGRLKFNIKLGLETPLDKRTLDPADFVAAIKYLLKLRKNIGAV
                     DDIDHLGNRRVRAVGELLENQFRIGLVRMERAIKEKMSVYQEMSTAMPHDLVNAKPVM
                     AAIREFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLSRERAGFEVRDVHPTHYGR
                     ICPIETPEGPNIGLISSLSCYARINEFGFIESPYRKVKGGRVIDYVQIVNAGDSEFKA
                     GEIVEKDKVDEMNEELKRRRIVVEPHCFYLSAWEEDRYVVAQANASLDEKLRITTDLV
                     NCRQAGNFVLKSREEVDYIDVSPKQLVSVAASLIPFLENDDANRALMGSNMQRQAVPL
                     IKGEAPLVGTGMERVTARDSGAVIVCRRDGIVDQVDSERVIVRVESDQSGVLSREVGS
                     DIYLLTKFKRSNQNTCINQKPLVRAGDRVKQGQVLADGPCTDRGELALGRNVLVAFLP
                     WRGYNFEDAILVSEKLVKEDYYTSIHIEEYEIEARDTKLGPEEITRDIPNINESFLRN
                     LDESGVIRIGATVKPGDILVGKVTPKGETTLTPEEKLLRAIFGEKAGDVRDASLYCPP
                     GIEGTIVDVKIFCRKGAEKDERHKAIEGAQVAKLEKNLADEIRILTDERLKRLSDLLG
                     GQKLQADLHDERTNKRLLTKGTELTRDILEKISTRNLKRLRLSDKDPLLIEKIDEIEE
                     MTSRQIDVLRKITDERIGKLKKGDELPPGVIKLVKVYIAMKRKLSVGDKMAGRHGNKG
                     VIARIVPAEDMPYMPDGTPVEIVLNPLGVPSRMNVGQILETHLGWASKELGERIGKLI
                     DGDSQVEAVRKWFKEAFGPTSVWRMISELDDDALLDLAHSFREGVPVATPVFDGAREA
                     DIRHLLEFTELPESGKITLYDGMTGDEFAQNVTVGYIYMLKLSHLVDDKIHARSIGPY
                     SLITQQPLGGKAQFGGQRFGEMEVWALESYGAAHILQELLTAKSDDVYGRAKIYEAIV
                     KGEPGIEPGVPESFNVLVRELQSLCLDVELMKRQKPQAEQSEAAD"
     gene            7968..12179
                     /gene="rpoC"
                     /locus_tag="VEJ00_05420"
     CDS             7968..12179
                     /gene="rpoC"
                     /locus_tag="VEJ00_05420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXX00634.1"
                     /translation="MYRSSPYDRASQIADFDSIRISLASPEKIRSWSHGEVTKPETIN
                     YRTFKPERDGLFCAKIFGPVTDWECLCGKYKRMKHRGVICDKCGVEVTLSKVRRERLG
                     HIELASPCSHVWFFKGLPSRIGYLLDITMRDLEHILYFETFVCVDPGEVPGIKEKDIL
                     PEEKFRELQRDNPGKFDARMGAEAIKELLRRVDVDKLSGELREKMKVDPSLQKRIKYA
                     KRLKVLEAFRKSGNKPEWMILDVIPVLPPELRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKKLMELHAPDVIVRNEKRMLQEAVDALFDNGRRGRVLRGTNNRPLKSLSDTLKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIYHRLEAAGHCT
                     TIKQAKELVEAQESTVWDILEDVIREHPVLLNRAPTLHRLGIQAFEPTLVEGKAIRIH
                     PLVCTAFNADFDGDQMAVHIPLSPEAQVEASVLMLSSHNILSPANGYPLAVPTQDMVL
                     GIYYMTKAKPKAKGEGRAFGTVEEVIMALEAGEVELLTPIRLRYTGEVIDLTNAYDDQ
                     DVSHTEPVQLNKQFLHTTAGRVIFNDHLPSDMPFINGLLKKKGIQALVQYGYLRYGLE
                     KTVQMLDRLKELGFLYATKAGVSIGIVDMVIPGVKSKLVDTAEHEVVKVQQQYLDGAI
                     TNGERYNKVIAIWSDVTEKVADEMFKALEQQDKEGVINPIYVMADSGARGSKQQIRQL
                     SGMRGLMAKPSGEVIETPITSNFREGLTVLQYFISTHGARKGLADTALKTADSGYLTR
                     RLVDVAQDVIINEFDCGTADGIYVEPILEAGVEVEPLRDRVVGRVSLEKIKDYEGNVI
                     VEINQEITEELANAIQAAGIERVKIRSVLTCESRRGVCARCYGRNLATGRFVEMGEAV
                     GVIAAQSIGEPGTQLTMRTFHIGGTATRVTEQSKVEARNNGFVKFIDLKAVEGRGKTL
                     IAMNRSGLIAVVDEKGREKERHHVVYGARLRVEDGQPVTLGQTMCEWDPYTFSILTET
                     GGTIEFKDLQPGLTIEEQVDEVTGLSQLVVTLPPGDEKHQPTIQVRDSAGRIRKRYLM
                     PSRAHLMVADGDDVQPGDVLAKIPRETTKTKDITGGLPRVVELFETRKPRDPAVISEI
                     DGVVKYGEIAKGQRKIYVESEDGRTQKEYSIPRGVHINVQEGEHVRAGEPLIDGPLNP
                     HDLLAVLGEKYTQAYLVNSIQEVYRLQGVNINDKHIETIVRQMMRWVKVEDVGDTSFL
                     LEEQVDKFRFREENDHVIADGGRPATGRPLLLGITKASLSTDSFISAASFQETTRVLT
                     EAAISGKVDYLRGLKENVIMGRLIPAGTGLERYRNIQLLTQLPPPEEAEAGFGAGLPG
                     GPELSPEEAAALDFLRKDTDAAAEG"
     gene            12447..14789
                     /locus_tag="VEJ00_05425"
     CDS             12447..14789
                     /locus_tag="VEJ00_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXX00635.1"
                     /translation="MSTDSSQRHPTEPEPPAPDESLAEVIARSQQNYRELIDHLDQAL
                     FTLSLTGEIRVANLRLAEILGVRFPELIGHSLGEFVESPSLADAERALPALMERGTWS
                     GTVAVRLKNEKEDRHFSCWLQTVTENGQVVSVTGWARDVSKEYEAQARFSELFESLRE
                     GIVFSTPEGKLVDANPALVRLLGYESKEELKRLGMRNLYADPAGREALMKELTEKGEV
                     HDYEIVLRRKDGKKLQCLTSGFAIRDASGRPVRMHGTVVDITERREIERRLHEEQEFA
                     RKLVECFPHLIVVLDRKGRFTFVSDRIRDVLGVSPEEYMGKSVGQRTDEGDQRKLAQM
                     HQDIVSGRKSQVQIEIRARHADGTWRSVQVNASPLFDEHGQISGVVSSGQDVTESRRI
                     EQQLAQKEKFTAMGQMMVGAAHELNNPLTAILGVTDLLRERATDDTTRRQVELVLKQA
                     RRAATIVQNLLAFSRPVTLGRTRLNLGDVVKEVLLLEAATLDQKKIRVTFDAPRDLPP
                     IEGDRKLLKEVFLNIIANAEQAMPPDRDHGTLAIKLSRVGNQVRVSFTDDGIGIPPES
                     MGKIFDPFFTTKRPGGGSGLGLTICLAVVKEHGGRIEAESAPGSGAAFHVFFPVLAES
                     SPEAPDSGSAAAGGAVRQKPLPAGSEVVQGHSVLVVDDEESIREIIQEGLVARGMKVD
                     AAANSEDALAYLAKNSCDVVLCDFNLPGMSGEKFFESLRSQGAEKLPRFVFMTGELLD
                     PDALERYRSRGAAVLQKPFHVSALVQLLGEILKAQPAQVG"
     gene            complement(14803..15705)
                     /locus_tag="VEJ00_05430"
     CDS             complement(14803..15705)
                     /locus_tag="VEJ00_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00636.1"
                     /translation="MFFLPLSIVLSMVPLRAQQAPSSPADSSSASQTSNGSSSRVLPR
                     GRKLLLKDGSYQLVREYRIDGDRVRYYSLDSSSWEAMPVDLVDWDATKKAEADQARRD
                     ATLAAKVEAQEAARVIPPLDIDASLEVAPGVFLPPGVNLFAFDGKSILPLPQAETSSK
                     ISKGRLLEQVMVPIPIVPSRQNVSIQGARAKFRLQVSQPEFYIRTADAREPRLELIRA
                     KAHGDSRLIEHIDQLFGEKAELRDSLPMQKWPVAQGVYRFTLDKPLEPGEYAFAEVLD
                     EEGMSLYVWDFGVDAGAVSGHKAN"
     gene            complement(15930..16139)
                     /locus_tag="VEJ00_05435"
     CDS             complement(15930..16139)
                     /locus_tag="VEJ00_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00637.1"
                     /translation="MVRIKSSQKFFTEEEVISLTGICAEHLRGLVQTRHLGSMAAAAG
                     SNVQKWLFTNSDLMIMAVLHPRCEH"
     gene            complement(16446..17891)
                     /gene="gatA"
                     /locus_tag="VEJ00_05440"
     CDS             complement(16446..17891)
                     /gene="gatA"
                     /locus_tag="VEJ00_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HXX00638.1"
                     /translation="MSALASWTVASVRVALEARKISARELATEFLGRIERRNRELNAF
                     LTVSPERAYAQADRIDAAIARGETLPPLAGVPIAIKDVISTRGLRTTCASKMLEHYVP
                     PYDATSVERLDKAGAVFLGKTNCDEFAMGGSNENSAYGPVRNPAALDRVPGGSSGGSA
                     AAVAAGLAVAALGTDTGGSIRQPASFCGIAGLMPTYGRVSRYGLVAFASSLDKIGPFA
                     ANVADAATVLGVIAGHDASDSTSADVLVPNYAAEVEKPVQGLRIGVPEDYFGAGLDSE
                     VKERIQAGIALLERLGCRRIPLQMPHTDYAIATYYILATAEASSNLARYDGVRYGLRV
                     PGGTLTGMYRNTREGGFGPEVKRRIMLGTYVLSSGYYDAYYLRAQRVRSLIAGDFTDA
                     FQKVDVIVTPTAPTPAFKIGEKSADPLQMYLADIYTVTGSLAGVPGISVPCGKTKAGL
                     PVGMQIFAPHFSEGSLFRIARAFESAGGFSV"
     gene            complement(17895..18230)
                     /gene="gatC"
                     /locus_tag="VEJ00_05445"
     CDS             complement(17895..18230)
                     /gene="gatC"
                     /locus_tag="VEJ00_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HXX00639.1"
                     /translation="MKISREDVIKVAGLAHLGLSSAEVELYREQLDAILTYVDKLKEL
                     DTSNVEPMAQVLHPAATSGGEEKHPELREDVLVPCDVAGHVLKGAPEAQAPFFRVPRV
                     IATGEVEGE"
     gene            complement(18695..19042)
                     /locus_tag="VEJ00_05450"
     CDS             complement(18695..19042)
                     /locus_tag="VEJ00_05450"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HXX00640.1"
                     /translation="MERAGDILGKALRRLDRPEAGLAWLSSAWPSIVGPALAAHTRPT
                     RCIAGRLDLAVDGKAWRQQLEPMQRELCGRINRAWGGNLVHEVKFLAARTAAKRLLHE
                     ADNDHTPFIRRRS"
     gene            19236..19310
                     /locus_tag="VEJ00_05455"
     tRNA            19236..19310
                     /locus_tag="VEJ00_05455"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19269..19271,aa:Glu,seq:ctc)
     gene            19669..19986
                     /locus_tag="VEJ00_05460"
     CDS             19669..19986
                     /locus_tag="VEJ00_05460"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXX00641.1"
                     /translation="MRAEFPKWTKHFQRSYPKVWKAFQKLGEECHSAGPLDARTRRLV
                     KVGIAAGYGSEGAVHSAVRNALAEGVSEDEIRHVILLSITTIGFPNGQAALSWAEDIV
                     RAK"
     gene            complement(20028..20480)
                     /locus_tag="VEJ00_05465"
     CDS             complement(20028..20480)
                     /locus_tag="VEJ00_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXX00642.1"
                     /translation="MIYSKPCEYAVRALVFLTRFPDRRAVQGAEIAEGEGVPAPILGK
                     VLQELVRKGLLESRRGPGGGFRLARDPQLITLRDVVAAIDGLDQFAECAVGLEHCSDE
                     SLCPLHETWKALRTHVMNYLQDTTLADMAAAVARKSNVRLRSKRARAR"
     gene            21269..22129
                     /locus_tag="VEJ00_05470"
     CDS             21269..22129
                     /locus_tag="VEJ00_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXX00643.1"
                     /translation="MTSVRIVRIGILLAGMCACLARPSVADVQDAGSFFRQNCSACHT
                     IGGGHIIGPDLQGVTKRKDRAWLVKFLQSPKAMIDSGDPYAGQLVQDAPGHMVMPTVP
                     GMNPDLANALLDYIEGGTASAKPQAAGPAISDRPFTVADVALGQQLFLGERPFENGGP
                     ACVSCHTLGTISGLGGGRLGPDLTNVFDRLGGRQGLGAWLSTPPTPTMQSVFGKHALQ
                     PEEIFPLLAVIDDANARSQPANQSSILKFFAFGFGGTLVGLALLQIAWRGRIQSVRRS
                     VVRTHARGAQ"
     gene            22126..25770
                     /locus_tag="VEJ00_05475"
     CDS             22126..25770
                     /locus_tag="VEJ00_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HXX00644.1"
                     /translation="MKHKDALSWIRDESTPQLRGWEEFYRNRWQHDKIVRSTHGVNCT
                     GGCTWQIYVKDGIVTWEMQGLDYPSLEAGLPPYEPRGCQRGISYSWYLYSPLRVKHPY
                     MRGALIDLWREARASYGDPVEAWASLVENPEARKRWQQARGKGGFRRADWATALELIA
                     ASCVYTAKKHGPDRINGFSPIPAMSMISYAAGSRFLQLLGGVSLSFYDWYCDLPSASP
                     ETWGEQTDVHESADWYNAKLLAVMGSNLNMTRTPDCHFAAESRHNGTKMWVFSPDFSQ
                     VSKYADEWVAINAGQDGAWWMAVNHVLLKEAHHQKQTPYFLDYAKRYTDSPYLVELHE
                     KGGVFQPGQLLRANRLGAYSGVENGDWKFLMWDAKDSRPKLPMGSSGFRWGKEKGKWN
                     LLLKDGVDGSEIDPVLTFLQSNDGVSQVRFDDFGEGRAVQRGVPTKKIRTADGRTATV
                     ATVYDLLMAQYGVSRGLPGDYPRDYNDDSQPYTPAWSEKYTGMSRDMVIRFAREWATT
                     AELTKGKCTVIIGAGVNHWYHANLMYRAAIHALMFCGCVGVNGGGLAHYVGQEKLAPM
                     EPWSAIAFARDWFPASRLQNAPSWHYIHTDQWRYEKSFTDYHTVPANQQEGSLASGHT
                     ADTQVRAVRNGWLPFYPQFNANPLDVVKQAESAGAKNEAEIYSHVVRQLRDGKLKFSV
                     EDPDAPENWPRVWFIWRGNALMASAKGHEFFLKHYLGTHTNAIAQDIAADSVQEIAWH
                     ETAPVGKMDLIVDLNFRMDTSALYSDIVLPAATWYEKADLNSTDMHSFIHPLSAAVPP
                     CWESKSDWQIFEAVAKSFSELAQQHFPKPVWDLVASPLAHDTPAEIAQPTIADWKEGG
                     IEPVPGKSMPGLKLVARDYSNLYNQFISLGPSVRANGLGAHGTHYPVDDTYDEALRTL
                     PTEEWGGKRYPSLAADEDACNLILRFATVTNGELAYRSYKDIEEKVGLPLTRLAEKSR
                     SVRVDYKGLQARPQRLLNSPMWSGLVEDGRAYSPFTYNVECLVPWRTLTGRQHFYLDH
                     EGYLQFGEHLPTYKPKPSPAQYADLRLSQEAGPTKMLNYLTPHGKWHIHSTYGDNHRM
                     TTLSRGVEPLWMNDKDAADINIADNDWVEVHNDNGVVVTRAAVSARIPRGICIQYHSP
                     ERTLSVPKSPLRKGRRAGGHNSLTRVRLKPNLMVGGYGQFTYHFNYWGPTGCNRDTHV
                     FVRKLPELQW"
     gene            25787..27277
                     /gene="narH"
                     /locus_tag="VEJ00_05480"
     CDS             25787..27277
                     /gene="narH"
                     /locus_tag="VEJ00_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HXX00645.1"
                     /translation="MNVRSQVSMVFHLDKCIGCHTCSIACKNIWTDRKGTEYMWWNNV
                     ETKPGTGFPTDWEDQDRYHGGWELHDGKLRLKSTGKAKTLFNIYHNPSMPTMDDYYEP
                     WTYKYEELFTAPAGTDQPTARPVSMVTGELINIEAGPNWDDDLGGSPIYAASDPNLKG
                     LNHDTQQQLFAIERLVFFYFPRICNHCLNPSCVASCPSGALYKRGEDGIVLVDQTRCR
                     GWRACVAACPYKKMFYNWNTGKSEKCILCFPRLETGQAPACFHSCVGRIRYLGVLLYD
                     ASRIEEVASLPSEKLIEGQRSLILDPNDPEVISAARRNGVHESVLESAKRSPVYRFVK
                     DWKLALPPHLEYRTMPMLFYVPPMSPVMSNRSNGSLDHVSDDLFHDIEEARVPMKFLA
                     NLFGAGEEGPLRYALRKQKAVRWYRRFKTVGDVDEQTAMRMLREADCSVEEAEAIYRL
                     TSLCTFDDRFVIPPMHREEAIQMMEDPLEHKQGVGFGFLSGPKRGM"
     gene            27277..27852
                     /gene="narJ"
                     /locus_tag="VEJ00_05485"
     CDS             27277..27852
                     /gene="narJ"
                     /locus_tag="VEJ00_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HXX00646.1"
                     /translation="MADANEFESLAALLRYPTEDYAREVVRSCKVLANDSPEIDLLLQ
                     HFVEQTRSLSLEDLQVLYTTTFDLDPVCSLEIGWHLFGENYERGEFLVRMRGELRRLG
                     VKESTELPDHLSHALEALGRMEPDEAADFAAACLFPALDKMAAGIKGKSNPFENVLSA
                     ATRLLEHRYPRPEPAVVLAEPAFRILDSKGW"
     gene            27861..28550
                     /gene="narI"
                     /locus_tag="VEJ00_05490"
     CDS             27861..28550
                     /gene="narI"
                     /locus_tag="VEJ00_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HXX00647.1"
                     /translation="MLPFANGLFFMVLPYLAMFVFFLGTIMRYRKAPFTYSSFSSQFL
                     ENKQHFWGLVPFHYGIVAVLLGHIVAFLIPREILAWNSRPLRLYVLEASALVFGLLTL
                     VGLCGAIHRRLTFSKVRAVTTPMDWIVFTLLLVQVASGVYVAVFHPWGSSWFAAAVAP
                     YLWSLVKLNPDLGFVTAMPLVVKFHIVMAYTLIGVAPFTRLVHILVAPNPYFWRKNQV
                     VRWYRAPEEIS"
     gene            28613..30106
                     /locus_tag="VEJ00_05495"
     CDS             28613..30106
                     /locus_tag="VEJ00_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HXX00648.1"
                     /translation="MAAATVLLCSALGWSSAVQGQSQSQSQSDPAQASGYKLGPLDIS
                     GNWRVRVEGWDWFEGNSGENSYAFPHSLLRVAIGQQTERFDWQVEAAQDAILFLPRHA
                     SVAAPAVQGQLGPGGTYYAANGNGRNNVNAFVKQAYFQINRLGEGKLKLGRFEYFDGS
                     EVKPNDATLASLVQTRISQRLIGNFAFSAVQRSYDGAQFSYNFDKKNLTLFGARTTRG
                     VYQIDGMGELDVDVYYGALTLPTSTEHSAGELRLFGLGYIDHRTGVLKTDNRSPDDRR
                     ADHGEIQIATYGGDYLHVFNTDASGKFDFLVWGAVQTGSWGRLTQRAGAFVGEFGWQP
                     RVKTLKLWLSAGYSYGSGDGNGSSNNSVHGTFFQVLPTPRIYARMPFYNMENNEDFYG
                     SLNLRPHSKLPMRSELHALRLASRNDLWYLGGGAFQPQSFGYTGRPSGGNRSFANVWD
                     LSADYQVTRNFSLGLYYAHAWGKAVIASIYPKDPNGQFAYVETNLRF"
     gene            30149..31501
                     /locus_tag="VEJ00_05500"
     CDS             30149..31501
                     /locus_tag="VEJ00_05500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX00649.1"
                     /translation="MDSIVGSPRKALWSATLGFFVGFAAVALFGPSANRFQQVMGLGP
                     LALGFLVAVPSLSGSLLRIPFSAWVDTTGGRKPFLWLLSLAVVGMVGLALVARFLYPT
                     HLTYKSYPLLMTLGTLSGCGIATFSVGISQVSYWFPQREQGRALAVFAGIGNVAPGIF
                     LMLVPFALTRFGLAVSYLLWLCILVAGTILYALLGKNSPYFQLLKKGLVPADARARAH
                     ELGQDLFPTGNLKDSLRISARVWRTWALVWIYFTTFGGFMALTAWLPIYWTRFFGVSV
                     EHALMLTAVFSIVASLIRVAGGVLSDQLQEGGENTAILALLITLVGALVMTSSRQFEL
                     SVPGEVLMAFGMGICNAAVFKIVPQAVPKAVGGAAGWVGGFGAFGGFVIPPVMGFAVR
                     NLGQSGYAIGFITFIFLALFSLSMAWILKYAREDVSPRDSTVRAKDAAVAPETARVTR
                     "
     gene            32347..34641
                     /gene="aceA"
                     /locus_tag="VEJ00_05505"
     CDS             32347..34641
                     /gene="aceA"
                     /locus_tag="VEJ00_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase ICL2"
                     /protein_id="HXX00650.1"
                     /translation="MSTLDQRVEATSRWIASPRFAGIVRLYSAREVAEQQGTISSDYT
                     VARQAAEELYTRLRELFKQRKQITTFGPYSPGQAVTLKRMGIEAIYLGGWATSAKGSS
                     SEDPGPDLASYPLSQVPDEAAGLVRALLAADKNQHFARARMTKEQRRATPTVDYRPYI
                     IADADTGHGGEAHVRNLIRRFVEAGVAGYHIEDQKPGAKKCGHQSGKVLVAEDEQIRR
                     LNAARLQLDIMRVPGIIVARTDAEAATFLDNRSDERDQPFILGAANTELPSYRTGYLA
                     ILRKLFELGVELRGHLLFNVSETEYQAAFAWLDRVGLASLIAKVGPGLVHASGIELDE
                     ALDKMDTRYMEIWQVEAGLKTYGEAVADVIKSRTDEGEPFETTVDKWLEFAKRASLYE
                     AHERARSMGIEVTWDCELPKTPEGFYQVQGGIDYAIAKSLAAAPFADILWMETKTADL
                     DDARTFAEAIHAKHPDKMLAYNLSPSFNWDTTGMSEEEMRRFPEELGKLGFVFNFITY
                     GGHQIDGMAAEEFATALKEDGMLALARLQRKFRLLESPYRTPQTLVGGLRLDGALMAS
                     SGRTAATKAMGKGSTHYQHLVQTEVPTRVLEEWLSKWTKHWKYPARIRVELRPHTAGS
                     ELLELNVVDAVSKEKLASIIFANAQDRRGRNFLLVRDQQTVARLRQKRVMTLVQLFLI
                     HRYKASAVHYLTPTQDNLFQTQGMKTIGIFSDVHTEVGQIIVAQVDKLRVSELLKPDG
                     TALSELICQKSPSVVKLEGKAHHR"
     gene            complement(34828..35736)
                     /locus_tag="VEJ00_05510"
     CDS             complement(34828..35736)
                     /locus_tag="VEJ00_05510"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXX00651.1"
                     /translation="MGIPGVSVIGDRINPGFKATKALVEAEDIKGLQALAVQQVQAGA
                     WCLDVTVGPRGYKDFKFLTEVINALQAAVDVPLCFDYPSAEVQAVCLKAYDPAKARGR
                     KPLVNSLAETRLEMLELVKIRPFQVILMASERLEDGAPKSNKLTIEVVGVARRLKDKL
                     TRDCGFSPQDLFVDVTIHSLVSDLEGLTKMALDAIRTIRQDPDLQGIHIIGGITNIGN
                     MLPPLKFDGIALRQLVENAFLTLAVPLGFDTIMGTPWNDFRTLPDDHVVLQTLKDFTE
                     LKGLNAMRRLRQLWAQGQESKSAAQK"
     gene            complement(35749..37656)
                     /locus_tag="VEJ00_05515"
     CDS             complement(35749..37656)
                     /locus_tag="VEJ00_05515"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HXX00652.1"
                     /translation="MRGPGAMPSPLAEDVWGRGMRSAQESSRTSHSITFEPDGQQIWS
                     LPTETLLDTAHRAGVRIASVCGGRGLCQSCVVRVTEGPVEPPSLQDIEFFSPEAIANN
                     WRRACQTFASGDCRVEVSERARAVPLRTEVESEDVWVRPDPAVRLYPVTVRPPTLTHP
                     IADDIRLMNALNEKWPGTCSRVDLDVLRLLPAVLRANGGQVVALIRFGEVIGVTPPVK
                     DPLPGLAVDIGTTNIGVLLVDLRNGRTLASRATENPQTKYGADVITRIGKARASSENQ
                     KKLHDLVVAEINAVAKELCESQSLNPERIADVVVAGNTVMSHLLLQLPVDYLGAVPFV
                     AAAASAADVKARDIGIKAAPGAYAHVIPNIAGFVGGDHTAMLLAIGADHEQKTIVALD
                     IGTNTEISLVHKGQLLTISCPSGPALEGRHILCGMRSAPGAIESVAISGDDVRIQTIG
                     NAPPIGICGSGVIDAAAQIYFAGIVSQTGRMTKEDPRVRVRDGQSEFILVDEGATKGK
                     AVVFTQRDVRAVQLAKGAIRAGIQVLLEDAGLREDEIDQVIIAGAFGNYIDLASALAI
                     GMLPDLPLDRFAQVGNAAGIGAKLALVSYPHRATAQALAASSQYFELAGTERFNKAFM
                     RAMRFPDRRKN"
     gene            complement(37596..38303)
                     /locus_tag="VEJ00_05520"
     CDS             complement(37596..38303)
                     /locus_tag="VEJ00_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00653.1"
                     /translation="MPLITYPRLSLEVEDIAASWGSRRERLASARVTAMLSELLAKID
                     AEGWVQPKASFRVWKVVDSARDWLGLEGGSRISSPALGHHLPGATHVAAGVCTIGSAI
                     EEHVRQMFASSDRLRGVMLDEIGTLMLFCLGDRLEEAMQAEAARYGLDASAALSPGDD
                     GFAIPQQAVVLELAAGADIGVSLTSGGMLMPRKSLSAVVGFGLRMPKESRAERCERCA
                     ARERCPHRWPRTSGVAA"
     gene            complement(38312..39571)
                     /locus_tag="VEJ00_05525"
     CDS             complement(38312..39571)
                     /locus_tag="VEJ00_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXX00654.1"
                     /translation="MSAGGAAASASALYQQRLKRLTDAIELREPDRLPAIFFSHFWAA
                     RYCGITCREAMCDYERLAAAVRKAVVDLEPDAYQPVHPLVAFGRTMEILDYKQLEWPG
                     HKGPAENVAYQYLDSQYMAPEEYEEYILDSTGFMLHKYMPHVAGALEPLAQSRMYPGV
                     LHTRIVQSVEAYARPEVRRALETLIRAGEEMPKMRAVHNRVAQELKDLGFPMAAYGTT
                     HPPFDVAADYMRGSMGAMLDLFRNKERLLILMVTLVTLIPRAGIEAVKAAGGNMVFIP
                     LHWGLDGFMSPKQFTTFFWPQFQKVLLALIEAGIYPQVLWEGDCTSRLDVIKDVPPGK
                     CVYFFERTDLAKAKEALRGAVLRGNVPASMLITGTPNDVREHCKKLFDVVGDGSGFIL
                     DAGVGIPNEAKPENVLAMFQCAKECVY"
     gene            complement(39568..40209)
                     /locus_tag="VEJ00_05530"
     CDS             complement(39568..40209)
                     /locus_tag="VEJ00_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HXX00655.1"
                     /translation="MDDRSKQLVQQISDMQEEDAVKLARAMLEGGYDAVKLLGHCREA
                     MEIVGKRYEQGEYFLPELMLAGEMLKAIGDMTKPLIKQDASGAKTAGTVIIGTVEGDL
                     HDIGKNIVTFMLEINGFKVIDLGIDVPVSNFIDSIKEHKPQVVGLSGFLTLAFDAMKK
                     TVEAIRAAGLRENIKIMIGGGQVDETVKVYTGADAFGLNAMDAVSLCKQWVPA"
     gene            40479..41822
                     /locus_tag="VEJ00_05535"
     CDS             40479..41822
                     /locus_tag="VEJ00_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXX00656.1"
                     /translation="MEGQPQGRAPAPQFEERAKAWKSAEGIDFISPAVRDSYERRVQM
                     FIDVITLKKPERVPVFPGMGFYPFVYAGVTAEEAMHDYGKLAYALRKFQTDFLPDTVS
                     GTALYGSGKILEILDYKLYRWPGHGVPPNEPYQCIEAEYMRADEYPRLINDPSDFFLR
                     SYLPRVLGSLAPLASIGPITDILELPSVGSALIPFGLPQVQELLRKLLEAGRVALEWI
                     EVCRAIDVEAMSTLGLAGLIGGFTKAPFDIIGDTMRGTRAIMLDKFRQPKAVLEAVER
                     FVPLAIDASVHSAKRSRSPVVFIPLHKGADSFMSTRDFQTFYWPTLKAVILGLIKEGL
                     IPYMFVEGAYAKRLDTITDPDIPPGRTIWVFDQTDLREVKKRFSGWACFGGNVPSSLL
                     VAGTADEVSEHVKRLIDDVGRDGGYILSTGATVDHAKAENLHAMIDTGKEYGVLR"
     gene            42194..44593
                     /locus_tag="VEJ00_05540"
     CDS             42194..44593
                     /locus_tag="VEJ00_05540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HXX00657.1"
                     /translation="MGAIVEVNREGAPWLQFAEPFNIAEYCVDRHVAEGRGDRVAIKT
                     ADQTVSYAQLLENVNRFGNALASLSIGRGDRVLMVVKDCPEFFYLFWGAVKIGAIPIP
                     LNGLAPTGDFEFVIRQSRCGAIFHSPDFAQTIEAALGASTSPPKTVLLVEGGERSISA
                     LARKSSSDLKAVPAKAEDDCFFLYSSGTTGVPKGVIHAHADIAVTSQLYTVETVGANE
                     DDAFFSVARLCFSYGMNVGMTGPLFVGGTAILDEHRPTPQSVIDLFRRFQPTVFGAVP
                     TFYGQFLASGLLSRADVPRLRRCISAAEALPPELHRQWLEVTGVPIMEGIGSTEVGHI
                     YISNHVDDIRPGITGKPVRGYQVRLIDEDGNDVAEDVPGRLLVKGQSVMKRYWNDPER
                     TAKAFMEGWYNTGDTFRRDAQGYYIYCGRSDDVFKVSGRWVSPFEVESVLIQNPRVLE
                     AAIIGRRDEHGLIRADAWVVLKDPSAGSTQAEEELRHFCEANLPRYKLPARIHIVEKL
                     PKTATGKIQRYKLRVTSEHEDRTPPQKREDALVSIERAGRVVTVTLSRHPVNALNDEF
                     IDRLEAVLDQVIKDEEVMVLHIRSDQKVFCAGADLALMQSCFGTLEGPDVMLDLVVRM
                     QRLYAKIEAAPLITLAEINGAAMGGGLEFALACDLRVAANEAKLGLPEVSLGLLPGAG
                     GTQRLTRLCGHGVASRLILSGEVIDGVMAERLGVVQWSQPQSQLGEWTRELSARLAKA
                     PRPALAASKRCLAAAGDPTRDGFAEELAATRKLYQHPETRHRVSEFLERSAKRHQTTK
                     A"
     gene            44629..45375
                     /locus_tag="VEJ00_05545"
     CDS             44629..45375
                     /locus_tag="VEJ00_05545"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXX00658.1"
                     /translation="MKLNGKSAIVTGAASGIGLATADTLAEAGALVVIADIARDKGEA
                     AAVGIQKKGHEAEYVHLDITDDKSIAAFVETVQSKVGAIDIIVNGAGWGQIKPFWEMP
                     LDVWSKIVELNLVGPMKLIKALLPKMMERNGGKIVNVGSDAGRVGSSGETVYAGAKGG
                     LIAFTKSLAREVARYNINVNCVCPGPTETPLLSAVPEKHLEAFKRAIPFRRFGKPQEV
                     ADAILFFASPRSDYITGQVLSVSGGLTMVG"
     gene            45398..46216
                     /locus_tag="VEJ00_05550"
     CDS             45398..46216
                     /locus_tag="VEJ00_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006217226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HXX00659.1"
                     /translation="MKQIQLANYKASHFGWSVSGKTATITLNRPERKNPITFESYGEL
                     RDLFRELVYATDIKAVVFTGAGGNFCSGGDVHDIIGKLVKMDMPELLAFTRMTGDLVK
                     AMRSCPQPIVSAIDGICAGAGAMIACGSDLRFGTARSKVAFLFVRVGLAAADMGACTL
                     LPRLIGMSRAADLLYTGRAMSGEEAERFGFYNRLVEPEKLLEEATATASSLADGPTFG
                     HGMTKTMLYQEWSQGLDECIEAEAQAQAICMQTKDFERAYVAFASKKTPVFEGN"
     gene            46216..47421
                     /locus_tag="VEJ00_05555"
     CDS             46216..47421
                     /locus_tag="VEJ00_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXX00660.1"
                     /translation="MADKSFLTWPFFDHGHRILADELELWAGESLPPLLKGSEDNLDS
                     VYACVRRIAAELGKAGLLRVCVPLAYGGARDDLDVRSLCLGREILGRASGLADFALAM
                     QGLGSAPITLFGREKPKREILPRVSAGTALAAFALSEPDAGSDVSAISTTAKRDGRFW
                     RLDGVKTWISNAGLAAFYVVFARTGEGPGTKGLSAFIVDAESAGLDASEHIDVIAPHP
                     LGTLRLSGCQVTDDSLLGQPGEGFKIAMATLDVFRTTVGAAALGFARRALDEAAGRAL
                     SRRMFGHLQSEFQLTQEKLADMAVSIDAAALLIYRSAWTKDVLGGRVTREASMAKLFA
                     TESAQQVIDTAVQIFGGLGVVRGVTVERLYREIRALRIYEGASEVLKLIIGSKVLEAA
                     AGGRGGASS"
     gene            47442..48239
                     /locus_tag="VEJ00_05560"
     CDS             47442..48239
                     /locus_tag="VEJ00_05560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX00661.1"
                     /translation="MDVDIKRPLARHHAVITGGGRGIGAAIAEALARSGASISLVGRN
                     SEVLLATAERIKNSYGVKVATASADVADEAAVRRALAALGNALGAPTILINNAGVAVS
                     APFLKADAEFWRKVLDTDLMGAVYCTQAVLPAMLEAKWGRIINIASTAGLAGSAYITA
                     YCAAKHGLIGLTRALAVETARTGVTVNAVCPGYVDTEMTAQNVANIMKKTGRSRDEVV
                     ASLVGGNPQGRLIQPAEVADAVIWLCGDNALSVTGQSIVIAGGEIQQ"
     gene            complement(48296..49366)
                     /locus_tag="VEJ00_05565"
     CDS             complement(48296..49366)
                     /locus_tag="VEJ00_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXX00662.1"
                     /translation="MLPIVEKHYRDATFPVELVPQLGELGFFGSNLNGYGCAEMSNVA
                     YGLVMQELERGDSGLRSFVSVQSALVMYPIHSYGSPPQKDKWLPRLQSGQAIGCFGLT
                     EPQFGPNPGGMLTRAVRRGDRYVLNGEKMWITSGSIADVCVVWAKCEDDRVRGFLVEK
                     GMKGFKAHDVHGKLSMRASVTSGLSFSDCEVPAENMLPGVDGLRGPLSCLTQARYGIG
                     WGAIGAAMACYDTALRYAKSRKQFANKPIASHQLVQDKLAWMITEISIAQLLALHVGR
                     LEDWARMHPSHVSMLKMNNVWMARETARMARDILGANGTVEDYCVMRDMNNLESVYTY
                     EGTHDIQRLIIGERITGIAAYS"
     gene            49657..51204
                     /locus_tag="VEJ00_05570"
     CDS             49657..51204
                     /locus_tag="VEJ00_05570"
                     /inference="COORDINATES: protein motif:HMM:NF006124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HXX00663.1"
                     /translation="MTSGALLQSSLVAVIGSGTMGAGIAQVAAASGHPVLLYDTRTEA
                     VSTAIQSVRQTFQKLCSKGNHSAESAEKASARLRAATNLQEVSGAGIVIEFVVEDLEV
                     KQQLFREVEKIVDEDCLLATNTSSLSVSAIAAALRAPQRLVGMHFFNPAPLMELVEVI
                     GGVATDQQFLAAVYATAAAWGKTPVYAKSTPGVIVNRVARPFYGEALLLLTEQAASPA
                     TIDAVMRESGGFRMGPFELMDLVGLDVGFAVSQSIFNAYFGDPRYRPSPIQQEMVRAG
                     YLGRKAGRGFYEHGEDARLAQPSTEPPAPKPNRVVVPSEGRLAGALTKRLAGTGAEIV
                     SGRPRNDCADWAEVGDTMIAVTDGRSATQRARETEHRNLVLADLALDYTKATRIAVTA
                     ASTCDEHAYLSAAGLLQAAGFAVSHLKDVPGIGVMRTVAMLANEAADAVNQGVATAAD
                     VDMAMRKGVNYPLGPLEWADELGLEIVQTVLMHIGAHYGGERYRISPLIRQLVWSGNK
                     FHGPHLQ"
     gene            51217..52422
                     /gene="pcaF"
                     /locus_tag="VEJ00_05575"
     CDS             51217..52422
                     /gene="pcaF"
                     /locus_tag="VEJ00_05575"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: protein motif:HMM:NF006551.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HXX00664.1"
                     /translation="MNEAIICDAIRTPFGRYGGSLSSIRTDDLAAIPIRTLIDRNPKV
                     DWQAVDDVIFGCANQAGEDNRNVARMASLLAGLPQDVPGTTVNRLCGSSMDAIGIAAR
                     AIKCGEADLIIAGGVESMSRAPFAMGKAETPFSRSAEIFDTTIGWRFINPTMKNKYAT
                     DSMAETAENVANDFHIARFDQDAFALRTQQKWAKAQAEGFYRSEILPIPIAQKKGEPK
                     TFDVDEHPRPDTTPDNLAKLKPIVKADGTITAGNASGINDGSCALLLASEAAVRKFKL
                     TPRVRVLATSVAGVAPRIMGMGPVPATRKILAKTGLSLSQMDVIELNEAFAAQALAVL
                     RALGLPDDDENVNPNGGAIAIGHPLGASGARLVTTAMYQLHRGKGRYALCTMCIGVGQ
                     GIATVLERC"
     gene            52544..53299
                     /gene="ric"
                     /locus_tag="VEJ00_05580"
     CDS             52544..53299
                     /gene="ric"
                     /locus_tag="VEJ00_05580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HXX00665.1"
                     /translation="MSSPTRITDNSGNTGRTVSDLVLEKPASARVFESLGIDYCCGGS
                     QTLAEACNAANLSIEEVTAALEKCEPTSAEKDWRGEPLADLARHIVEKHHTFTQAEIS
                     RLSALIAQVVAAHGKNHPELPRLQTVFAGLSEELREHMRKEEELLFPYVVEMEEAART
                     RRRLPDSMFGTVQNPVAAMIMEHEASGQALEKMQEIAHNYALPPDSCASYRALYQALP
                     AFAADLHQHIHLENNILFPRAVELENSLSGSAR"
     gene            53347..53814
                     /locus_tag="VEJ00_05585"
     CDS             53347..53814
                     /locus_tag="VEJ00_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXX00666.1"
                     /translation="MKTAAAKGKTAKPEIAGPNVDAVLRIMDAMEKAWSDKDLPQLFD
                     HYWHNDGFVLFTSRGAYYGWEAAKQMLEQYFDNLEEVSLKFGPRKARVFGDVATIVYE
                     WRTDGRSTLNGSRLLREGYGTDVFLRDHETWKLYHNHVSLGRAGTSHMQDHAW"
     gene            53913..54308
                     /locus_tag="VEJ00_05590"
     CDS             53913..54308
                     /locus_tag="VEJ00_05590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX00667.1"
                     /translation="MIALDEEVTGIAKRKYWKEAFARYGARRRLPRFFLIAEQNGRIE
                     GFTIGEVRAWEFGEPPCGWVFAIQVRPGSRLNGVGTRLFEALCNEFRKAGVKRVRTMM
                     ARDNHLVLSFFRSQGMMAGPFIELESALD"
     gene            54330..54794
                     /locus_tag="VEJ00_05595"
     CDS             54330..54794
                     /locus_tag="VEJ00_05595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXX00668.1"
                     /translation="MKLQKASAFAIYAVLELAANPLRQLAGIDIAREYGISAHHLAKV
                     LRDLGRAGIVEAVRGVGGGYRLSVNPKRLTLLDVIQVFEPVGPQNDTTEDPELATDAG
                     QALRMVLDELDDHALATFQSITISTMLKLMARRPWAGSARVSKQGPRAASRH"
     gene            54826..56007
                     /locus_tag="VEJ00_05600"
     CDS             54826..56007
                     /locus_tag="VEJ00_05600"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HXX00669.1"
                     /translation="MASTSVQEISTLDSTLASCREIAQDPEFSTAKRWLEEHPGKKAI
                     GCFPIYCPIELIHASGMLPLGVIGAGNQIEITHADSRFQSFVCSIVKSTLELGLTQRL
                     KFLDGMLFHSICDPARNLASVFQRNFPEMFVEYIHFPQNFASVLARQYLETEYRRILQ
                     GLQQRSGHTPTTEDFNHSIGLYNRIRAALRKIYQIRTDTPHLISAAESFVLMRAGTLM
                     APEDFLKILEQVLAEIPQRPSRPKDRIRVVLEGSFCELPPVGLIETLEQAGCYIVDDD
                     FLRGWRWFTDDVPIQDSPLAALAESYSERAYYSGTKHDTRETKAAHLIRLAKEKKADA
                     VVFQAAKFCEPALFDYALYRKALEDAQIPHLLLEFEEKTWSFDRARSEAETFVESLLF
                     D"
     gene            56037..57317
                     /locus_tag="VEJ00_05605"
     CDS             56037..57317
                     /locus_tag="VEJ00_05605"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HXX00670.1"
                     /translation="MAQAPAAAKTDYQGRIHNLQKDMIGRYYSRLTAAAENKSEKAIY
                     MLISGNPVELTEAFGLIPVYPEVNALQLAVKKQSLPLILAAEEMGYSTDNCAYVKADV
                     GMYFAGRKTPFATIPEPDLILCNYVGCNVYLQWFEHLAEYSKAPIYNLDVPFFRTTSD
                     DPSEEDVRYVVKQLEELVEILERMTGQKLDYKKLQRIVELSGETGALWSEIKRLTMER
                     PAPYDAYFDSVMMMAPLYCLRGTEDGLWFFQETYKEMKKRAESHEGPLPEEKFRLVME
                     GPPPWPYLRQFRDMFSRWGAVAVASTYSTVGGIWEFGFRHDPLRPLESIARHMLQWNM
                     TNRNFRQRYQQIKKYVEDWKADALVIHSVKSCRLFSAGQGDMREYFSKQLGVPTLLIE
                     SDLEDPRYFSEAQLRNRVDAFFEALEHQKMLAAK"
     gene            57372..58232
                     /locus_tag="VEJ00_05610"
     CDS             57372..58232
                     /locus_tag="VEJ00_05610"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HXX00671.1"
                     /translation="MTYGGGVDVGSTQTKAIILSDDRRIVGRALINTGANVSQAAERS
                     FREAAASAGIAPEKVAYVVGTGYGRYKVNFGNAQITEISCHARGANFLFPNTRTVIDM
                     GGQDAKGIRVGENGDVKDFVMNDKCAAGTGRFLSNAAETVGLSLDKIGPISLEAKKPV
                     RLSTVCAVFVESDIMSYLAEGKTVPDILGGVHSAIAARTVALVRRVGIEPEIAFTGGV
                     SRNIGMVKALEEKLGLPVNVCDDSHFIGAIGAAIFALERALGGETGAGSAKANPVAAS
                     QASQPSAGAH"
     gene            58272..59102
                     /locus_tag="VEJ00_05615"
     CDS             58272..59102
                     /locus_tag="VEJ00_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HXX00672.1"
                     /translation="MAYVVGIDIGSRSTKAVILDESRHVCGTGLVKTRPDFPGVAKEA
                     MEQALAKAGAQKEDVKYVATTGLGRYNIPFRDLQITDITCAARGAEFLFPNTSCVLDV
                     GGQSTRAIRLRAGGKVKEFKSNDKCAAGSGGFLERAAHYLEIPLTQLGPLSTKAEKPT
                     TISSVCAVLAESEIINHVSEGQSVENIIRGIHNSLASRSKALLSRVGLEDEVTFVGGV
                     ARQSGMVEALKQTIGRDVNVAEEPDTVAALGAALLAHQRLQKLGGISKEKGTDPYVYG
                     "
     gene            59446..60987
                     /locus_tag="VEJ00_05620"
     CDS             59446..60987
                     /locus_tag="VEJ00_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00673.1"
                     /translation="MNTAPAGFDPATDLPKGFVDFLLPLVQPFTRRQRELVQKRAAAL
                     TAAHEGKLPDYLPPSPATKDDWQIELPDWCRDQRNQMTGPADDAELVVKMLNSGAPGV
                     MLDLEDSMANTWPHLMQGVANVLAALGGTLSYFDRKRNRTVSIQPSKSAIFVRPRGLH
                     IHQAGVLPGEILPASLFDVALIAFQVNPGEMKHPLTFYIPKTESAEEGLWWRDLFQVV
                     AKARGWRKDEIRCMALVESHPLAYQMEEFAYNLREHMLALNLGRWDYMASLIHFNLRD
                     PRWVLPDRNTIPHDVSFFQNIRNRMPEICHKRGMLAIGGMTALYPSREDAELNARALA
                     MLEKDKKNEALCLMDGAWTGHPDQNEIAVKQFPFPNQLHARPSDPEPRPDLRPIPEGV
                     GKRTLAGTRAAVRTVIRYRQGVLAGRGASLLDGYMEDLATDRIYRLMIAQRIRHRDSV
                     PIVDDGGQPVPHTPQRITQLFDEELSRILRELPGDASPEIGEQFRQARALSEKLIVSG
                     EFTPP"
     gene            complement(61094..61561)
                     /locus_tag="VEJ00_05625"
     CDS             complement(61094..61561)
                     /locus_tag="VEJ00_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXX00674.1"
                     /translation="MSPLVIEAPQALSDFVGREIAVTEWFAVTQEMIRQFAEGTDDRQ
                     WIQVDPDRAQRESPYGTTIAHGFLTLSLLSRFLREAIQVQGGVRMRVNYGLNRVRFPS
                     PVRAESQIHARVSVQSTKQLPDAIEATYSVVLECRGAEKPCCVAECIVRYYFG"
     gene            complement(63098..63601)
                     /locus_tag="VEJ00_05630"
     CDS             complement(63098..63601)
                     /locus_tag="VEJ00_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXX00675.1"
                     /translation="MIVREMSGEECRGLLARAEYGRLATVHQDQPYIVPIYFAYDRDY
                     LYGFTTLGRKVEWMRANPLVCVEVDDVVSHFHWSSVVVLGHYEELPDTPVYRDVRLQT
                     QVRLERRVLWWQTAYAAGQVRGGFQPADALFYRIHIDQITGRRATPDPVESAVGLSSA
                     ARPAREK"
     gene            63738..63896
                     /locus_tag="VEJ00_05635"
     CDS             63738..63896
                     /locus_tag="VEJ00_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00676.1"
                     /translation="MSFKILRELENGELLEVANHGDFEKAEALVRALNEHWPGVYLIR
                     EIENEESE"
     gene            complement(64602..64832)
                     /locus_tag="VEJ00_05640"
     CDS             complement(64602..64832)
                     /locus_tag="VEJ00_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00677.1"
                     /translation="MTETCESSLRNDAFVPLLPPPARSVVMRPWESAPEKLTKKRSLA
                     AAAFLAVYLAAYIGVGFAGVALLEIFWSAITQ"
     gene            65076..67292
                     /locus_tag="VEJ00_05645"
     CDS             65076..67292
                     /locus_tag="VEJ00_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXX00678.1"
                     /translation="MRPVAKVLVATTAMLAFISYWRAAAIVLNDLGSSAFYAGAIAEH
                     FIGKTAPWFVLAIMLFAFAVQALYFESCGMFVRGGVYRVVKEGMGSTLAKFSVSALMF
                     DYILTGPISGVSAGLYLVGLLNELFQYAHLKIALPVNAGAAIFAIIVTLYFWWENVKG
                     IPESSEKALRIMYVTTVMVIMMIAWCGYTLMVRGGHVPPFPHISNLVYAPDSLGWLKH
                     TSLPYTIGFIGIMIGLGHSVLAMSGEETMAQVYREIEHPKLPNLKKSGLVIFLYSLIF
                     TAGVAFFAVMIIPDSVRARFSDNPISGLAMYLVGPLSARLAFRTFVVVVGVLMLAGAV
                     NTAIVGSNGVLNRVSEDGILPDWFRHPHRRFGTSYRILNLVVGLQLLTIILSRGNIFV
                     LGEAYAFGVMWSFAMKGLAVVVLRYKQPGQREFKVPLNPKIAGVEIPIGVGLITLALL
                     ALCLINLFTKQVATVSGIVFTIAFFAVFEISEKMTKKQATSHAELDKFNLAQSGDLTL
                     ENVGVKPGNVLVPVSNYYALYPLEAALKRAKRREAEIVVLHVRLLGRAASGESDLAPD
                     QLFSTIEQSLFTKVLAMAEKEGKPVRLAVAAANDLWEGILRTAANLESSTVVAGSSAK
                     ASVAEQAREIGLAWERMPEPRPRVSLEIFAPSGTEQVFYLGPHAPRLTPNEIDLLHRI
                     WLDLSDRLPGEEIHHHDIVHFALSEVEREIGEKERDGVLRRLREHLSGIQGRRQNP"
     gene            67464..67540
                     /locus_tag="VEJ00_05650"
     tRNA            67464..67540
                     /locus_tag="VEJ00_05650"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:67498..67500,aa:Arg,seq:tct)
     gene            67925..68455
                     /locus_tag="VEJ00_05655"
     CDS             67925..68455
                     /locus_tag="VEJ00_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00679.1"
                     /translation="MISRLAILPLVLAAAFVLGCSKESQPSQMPASSAAAPDASSATM
                     PSAPGSSAPASGSDADQIRQAVIDHVRNDRGVNMSAMDMSVDLVNVNGDQAQANATFR
                     VKQGGATMTMVYSLQRHGNGWLVVNGQPSDGQFVHPPMDRTHSGVSASPGAPEMPDVR
                     EFLKTHPSTTGQPEAH"
     gene            68621..69220
                     /locus_tag="VEJ00_05660"
     CDS             68621..69220
                     /locus_tag="VEJ00_05660"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HXX00680.1"
                     /translation="MTSPVKKALRPLLRTGILCCVVATMCKPALSITLESRKPAEHRL
                     RMFNTHTGEQIDIVYRRGNDYVVDALAQLDYFLRDHRTGDVHHFDPRLYDVLERLTAA
                     VGRLGAEIDVICGYRTPWSNNFLRTHSPGVAEHSLHMQAEAIDIRMPGVNSERLREAA
                     LHLHEGGVGYYPHSNFVHVDVGRVRQWCFGCTGEQMHAD"
     gene            complement(69217..70788)
                     /locus_tag="VEJ00_05665"
     CDS             complement(69217..70788)
                     /locus_tag="VEJ00_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXX00681.1"
                     /translation="MRSIIESGRLDDLRWPEFRGYQADIATFYQSYRYALPWIRDMQA
                     TNQARAVIEALSRADEKGLSPDDYDGPRWADRLARLQPNTSQTDEAGTVRFDVALTVS
                     LMHYVSDLHTGRIDPHLFGIDVNIAKRKCDLPKFLSEQIVPAADVRAALLRVEPSYPG
                     YERTIRALETYRRLAKEKTGQPFPATNRAIAPGQSYSSAPRLRQFLQLVGDLAADAPI
                     PSDETKYDSALADAVRRFQERHGLTPDGRIGPKTLEELNVPLESRVRQIELTLERWRW
                     LPAQYGEAPIVVNIPGFMLRAYDDAFHVAVTMKVVVGRAYDHETPIFMSRLQSVVFRP
                     YWDIPVSIAREEIVPAIEDDPEYLAKQNMEIVNGRGEVVATDTVNRHLLNQIGAGSLS
                     VRQKPGPNNALGLIKFQFPNEYSVYMHDTPARLLFSRSQRDFSHGCIRLEKPEELAGW
                     VLRDNPGWDPDRILAAMNGEATLEVKLVHPIPVLVVYGTAIDLEDGIVRFYDDIYGQD
                     AKLEEALRSEYPYPH"
     gene            complement(71368..72708)
                     /locus_tag="VEJ00_05670"
     CDS             complement(71368..72708)
                     /locus_tag="VEJ00_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HXX00682.1"
                     /translation="MGLPTAPLVTIAFKDLAKTNAANRGMPGERICFLPHPMTNKTDE
                     EMYAVLGGNDPVTNKPLMPEIMDALTKPLTADEQKSGQVTPDIGPATYVGTPNDLQRI
                     YLDNNMTDFMPIILPTEEKVQAMLKGTSHKPDEVIGTLSAARGAQPSWSFTVRHVAIN
                     AVMAGATPEYLPVILAIASTEQPALFSSTSSFSRMLLLNGPISDQIKMNYGIGAMGPF
                     AEPNAVIGRAWTILSKNLGGCGMPGLSYMGTLGSNFNYNNICIAETENKLPDGWKPFH
                     VQKGFKPDDSVVSIFSGWSLNNVAWFPNMPYHELIKNWLTHFFSFGTSAATVLLDPTI
                     AREAKGKGFNSKEELSNYWSKESKTPAWLYWDTHKNELKQAQAGVEPYASYLKLGDGE
                     IPFQRFYRRGRPGGPPDPNPKSSIEVIVLGGETNTYYAGGDFSYVTSASIDKWR"
BASE COUNT        15379 a        20781 c        22036 g        14566 t
ORIGIN      
        1 agcgagcgcg ggcgaagcga gaatggcgga agcactcaag atggacgaga aggaaatccg
       61 cggcgggaac ggcggaggcg cgggaagcgg ccttaccacc ggcctggcga agttcgggga
      121 gtacccgcgg aggctgcgga cctttctcca cgacgtgcgc gtggagatga agcaggtgaa
      181 ctggccgtcg cggcaagacg tctggtccac gacgatcgtg gtcaccgtca cggtagcgtt
      241 tttcggcatc tatttctgga tcacggacgg gattttcggc cggctctaca ttctggtgaa
      301 taattacttt caccatcact gagatgacag acgttatggc aaagcagtgg tacatcgtgc
      361 acacgtactc cggcttcgag aagaaagtga aggaaagcct cgaaggccgc gtgcaggcgt
      421 tcggcctgca ggacaaaatc ggtcaggtca tcatcccgac cgagtccgtg gtcgaagtgc
      481 gcggcggcaa gaaagtgatc tcgccgcgga tgtgctatcc gggctacgtg ctggtcgaaa
      541 tggatatgga cgactacacg tggcacgtgg tgcggtcgac tccgaaggtg accggcttcg
      601 tcggatcggg acaatatcct tcgccgctca ccgacgagga agtgcagaac attttgactc
      661 gcgcgacgac gaccgcggag aagcccaagc cgaagttgtc gttcgagcga ggcgagaagg
      721 tgcgaatcgg cgaggggccg tttgcgaatt ttaccggcgt ggttgaagaa gtgaacagcg
      781 accgcagcac gctgaaagtt tcggtgacga ttttcgggcg atcgacgccg gtggagctgg
      841 atttcgcgca agtcgagaag gtcgcttagt gatgacgtcc ttccaacccg gcgacagagt
      901 tcgaatcaac gacggcgtgt tcatgcattt cattggcgtc gtcacggaag taaacgtcga
      961 gcggaagatg ctaaaggctt cggtcacagt tttcgaccgc ccgcttgcaa tcgagtttcg
     1021 gttttcacag gtgaagaagg ctgcgtagca ggggatagag gatggcgaaa aaagtacaag
     1081 caatggtgaa gctgcagttg ccggctgcga aggccacgcc ggcgccgccc gtcggcacgg
     1141 cgctcggccc gcacgggctg aacatcatgg atttctgcaa gcagttcaac gccaagacgt
     1201 cgaaagagcc ggacggcatg atcattcccg tgctcgttac ggtctacagc gaccgcacat
     1261 tcacgttcat tttgaagacg ccgccggcgt cggagctttt gaagcgcgcg gcgggaatcg
     1321 tgaagggttc gcccgagccg aaccgcaaca aagtggcgaa ggtgacgaag aagcaggtcc
     1381 aggaaatcgc caagacgaag ctgcccgatc tgaacacgac aaatctggaa gcggcgatgc
     1441 ggtcggtgat gggcaccgcg cgcaacatgg gaatcgaagt tacggactaa ggggagaagc
     1501 gatgggccgc agcgcaaagg taggcggaaa gaatatcgat cgcgcccgga agctcgtcga
     1561 ggcgcggccg tatgccttgg aagaagcggc ggaactgctt cgcaaggcgc atttcgtgaa
     1621 gttcgatgag acggtcgagc tggtgctcaa tctcggcgtc gatccgaagc agtcggacca
     1681 aatggttcgg ggaacggtcg tgctgccgca tgggcttgga aaatcgaagc gcgtgctggt
     1741 gatcgcgagc ggcgaaaaag tccgcgaggc gcaggaagcg ggcgccgatt tcgtcggcgg
     1801 cgaagacatg gtccaaaaga ttaccgaggg ctggaccgat tacgaagcgg tgatcgccac
     1861 gccggacatg atgaagtccg ccggaaagct cgggaaagtg ctcggaccgc gcggattgat
     1921 gccgaatccg aaaaccggca cggtcacgtt tgaagtcgcg agggccgtga aggaaatcaa
     1981 ggccggaaaa gtggaattcc gtctcgacaa gacgggattg attcactcgc cggtcggcaa
     2041 gatcagcttc ccggcgaaaa atctggcgga aaacgcacag gcgctgctgg gcgcggtggt
     2101 gaaggcaaag cccgccgcgg cgaagggcaa atacatcaag aagattacgc tcacatcgac
     2161 gatgggaccg ggaattccga tcaacatcga agaggcagaa gccgccacag cggtggctgc
     2221 gtagttagtt tgaatgaggg agagttcggc atgaagaaga aggccgaaaa aaagacgcag
     2281 gccgaagaac tgagggcgca actggcgaca gtttccaccg tgattttgtc gacgtttcag
     2341 ggaatcacgg ttgaggacga caccagactg cgccgcgcgg tggaagcagc gggcggccac
     2401 tacgaagtgg tgaagaacac gatcgccgag cgcgcggcgg caggaactcc gaccgaaggg
     2461 ctgctcaaga acctcaaggg cacgaactcg atcgcataca cgaagaccga tccggtttcg
     2521 ctcgcgaaag tgctgacgaa gatcgcaaag gacgtgcccg cgtttcagtt ccgcgcaggc
     2581 tgggtcgaag gtcgcgtcat ctcgattcag gagatcggac agctcgctac gcttccgagc
     2641 aaggaggagc tggtcagcaa gatcatgttc atgctgaacg ctccggcgca gcgcgtggcg
     2701 acggcgctgg ctgcgctgcc gcggaatatg gcggtggtga cgagcgaagc cgtgaaggcg
     2761 aacaagtttg gaggaggcgg cgcggcaccg gaagaggcgc ctgcaccgca acccgcgcag
     2821 taaaacgaga ttgatcgaaa cgattttaac tattgaggag gatagggact gcaatggcta
     2881 gcaaagtgga aacgattctt gaggaaatca agggcctgac gctgctggag gcttccgaac
     2941 tggttaagaa gatggaagag gccttcggtg tgtcggcagc cgcggcggcg ccggtggtga
     3001 tggcgggcgg cgcggcggca ggagcagcgg caggcgcgcc ggcggaagag aagacggaat
     3061 tcacagtggt tctctccgaa gtgggcgcca acaaaatcaa cgtgatcaag gcggtccgcg
     3121 aagtcaccag cctgggcttg aaggaagcca aggatttggt cgacggcgcg ccgaagacgg
     3181 tgaaggaagg cgtcaacaag gacgaagccg ccacgatcaa gaaaaagttc gaagaggccg
     3241 gagccaaggt cgagatcaag tagtggcaga ccgcgttcgg ccacgcggga ctgcaaagga
     3301 acacgcgtac gcacgcgatc ctttgaaaaa gttccacaaa acacgtccat cccggaccgg
     3361 cagaacgggt gtatgcccgc gcactgccgt attcggcttt gccctgaatg aggattgtcc
     3421 atgactcaac ggaatggtaa tcgcgtcgta cgggaacggg agcgtttgga cttcgccaag
     3481 atcaagacga ccgtccagat cccgaacctg atcgacgtgc agaagaagtc gtaccagcgg
     3541 ttcctgcaga tggatctgct gccgagcgag cgcgaggact cgggtttgca gtcggtgttc
     3601 acgtccgtct tcccgatcca ggatttccgc gggctttcgc agctggattt cgtcgactac
     3661 gcgatcggca actgggagtg caagtgcggc cacctgcgcg gcctgcatca cttgcgcgcc
     3721 acctgccgga attgcggcgc gtcagtggtg acgaacccgt acaaaacggg cgacgtgatc
     3781 tgcgcgaagt gcggcacgtt caacaagaac acgccgacgt tctgcaacaa gtgcggcgac
     3841 ccggtgacgc tgcaactgaa atacgacgtg aacgagtgcc aggaacgcgg catgacctac
     3901 tccgcgccgc tcaaggttac tatccggctc acgatttacg acaaggatcc tgacacgggc
     3961 gcgaagtcga ttcgcgacat caaggagcag gaagttttct tcggcgacat tccgctgatg
     4021 acggagaacg gcacgttcat catcaacgga accgagcgcg tgatcgtgag ccagctgcac
     4081 cgctcgcccg gagtgttttt cgagacagcc aacaaccgga catatttcct cggcaagatc
     4141 atcccctacc gtggctcgtg ggtcgaattc gaatacgaca cgaagaacgt tctctacgtg
     4201 cgtatcgacc ggaagcggaa attcctcggc tcaattttcc tgcgcgcgct gggcatgaag
     4261 tcgaatgaag acatcctgcg cacgttctat caggtggagc gcatcaacct gcgcgaaggc
     4321 gatctcttct ggaacgtctc ggaaggtttg atcgaccgca agctgtcgaa ggaaatcaag
     4381 catccaaagt cgaacgaagt gctggtcggc gcgcacaagc gcatttccga atcgctctac
     4441 aaggagatgg tgaaggcgcg cgtcaatcag gtggaagtcg gcatcgccga tctcgaaggt
     4501 gcggtgacgg tcagcgagct cgtgaaccgt gaaaccggcg aagtgctcct cgaggcgaac
     4561 aagccgctca ctgccgaaat gtgggccacg atcgcggagg cgggaatcac ggaagtagac
     4621 gtttgcttcc cggagcgcga cgacgtcggc atcatcgttt cgcgtacgct ggaaaaggac
     4681 acgatccaca cgccgaagga agcgttgatt gaaatttacc gcaagctgcg tcccggcgat
     4741 ccaccgacct tggaaacggc aaccgcgctc ttcaacggga tgttcttcga cgcgcggaag
     4801 tacgatttca gcaaagtcgg ccggctgaaa ttcaacatca agctcggtct cgagacgccg
     4861 ctcgacaagc gcacactcga tccggctgat tttgtcgctg caatcaagta cctgttgaag
     4921 ctgcgtaaga acatcggcgc ggtggacgac atcgaccatc tcggcaaccg gcgcgtccgc
     4981 gcggtcggcg agctgctcga aaatcagttc cgcatcggct tggtccgcat ggagcgcgcg
     5041 atcaaggaaa agatgtccgt gtaccaggaa atgtcgacgg cgatgccgca cgatttggtg
     5101 aacgcaaagc ccgtgatggc cgcgatccgc gaattcttcg ggagctcgca gctctcgcag
     5161 ttcatggacc agaccaaccc gctgagcgaa atcacgcaca agcggcgtct ttcggcgctg
     5221 ggccccggtg gactttcgcg cgagcgtgca ggattcgaag tccgcgacgt tcacccgact
     5281 cactacggcc gcatctgccc gatcgagacg ccggaaggcc cgaacatcgg tctgatcagc
     5341 tcgctatcgt gctacgcgcg gatcaacgaa ttcgggttca tcgaatcgcc ctaccgcaag
     5401 gtgaagggcg ggcgcgtgat cgactacgtg cagatcgtca acgcgggcga cagcgaattc
     5461 aaggcgggcg agatcgtcga gaaagacaaa gtcgacgaaa tgaacgaaga gctgaagagg
     5521 cggcggatcg tggtcgagcc gcactgcttc tacctctcgg cgtgggaaga agatcgctac
     5581 gtggtcgcgc aagcgaacgc atcgctggat gaaaagctgc gcatcaccac cgacctcgtc
     5641 aactgccggc aggccggcaa cttcgtgctg aagagccgcg aggaagtgga ctacatcgac
     5701 gtgtcgccga agcagcttgt gtcggtggcc gcgagcctga ttccgttcct cgaaaacgac
     5761 gacgcaaacc gcgcgctgat gggatcgaac atgcagcgtc aggcggtgcc gctgatcaag
     5821 ggcgaagcgc cgctcgtggg aacgggcatg gaacgcgtga ccgcacgcga ttccggcgcg
     5881 gtgattgttt gccgccgcga cggcatcgtc gaccaggtgg attccgagcg cgtgatcgtg
     5941 cgcgtcgaat ccgaccagtc cggcgtgctg agccgcgaag tcggatccga catctatctg
     6001 ctcacgaagt tcaagcggtc gaaccagaac acctgcatca accagaagcc gctggtccgc
     6061 gcgggcgacc gcgtgaagca ggggcaggta ttggccgacg ggccgtgcac cgaccgcggc
     6121 gagctggctc tcgggcggaa cgtgctcgtc gcgtttctgc cgtggcgcgg gtacaacttc
     6181 gaagacgcga ttctcgtcag cgagaagctg gtgaaggagg attactacac ctcgattcac
     6241 atcgaggagt acgaaatcga ggcgcgcgat accaagctcg gtcctgaaga aatcacgcgc
     6301 gacatcccca acatcaacga aagcttcctg cgcaatctcg acgagagcgg cgtgattcgt
     6361 atcggcgcga ccgtcaagcc gggcgacatt ctggtcggca aggtgacgcc gaagggcgaa
     6421 accacgctca cgccggaaga aaagctgctg cgcgcgatct tcggcgagaa ggccggcgac
     6481 gttcgcgacg cttcgcttta ttgcccgccg ggcatcgagg gaacgatcgt cgacgtgaaa
     6541 atcttctgcc gtaagggcgc ggagaaggat gagcgtcaca aggcaatcga aggcgcgcag
     6601 gtggccaagc tcgaaaagaa cctcgccgat gaaatccgca ttctcactga cgagcggctg
     6661 aagcgcctct cggatctgct cggcggccag aaactgcaag ccgacctcca cgatgagcgc
     6721 accaacaagc gcctgctcac caagggcact gagttaacgc gtgacatcct ggagaaaatc
     6781 tccacgcgca acctgaagcg cctgcggctg agcgacaagg acccgctgct gatcgagaag
     6841 atcgacgaaa tcgaagagat gacgtcgcgt cagatcgacg tgctgcggaa gatcaccgac
     6901 gagcgcatcg gaaagctcaa gaagggcgac gagctgccgc cgggcgtgat caagctggtg
     6961 aaggtgtaca tcgcgatgaa gcggaagctc tccgtcggcg acaagatggc aggccgacac
     7021 ggaaacaagg gcgtcatcgc gcggattgtt ccggcggaag acatgccgta catgccggat
     7081 ggaacgccgg ttgagatcgt gctcaatccg ctgggcgtgc cctcgcgtat gaacgtcggc
     7141 cagattctcg agacgcacct cggctgggct tcgaaagagc tcggcgagcg catcgggaag
     7201 ttgatcgacg gcgacagtca agtagaagcc gtccgcaagt ggtttaagga ggcgttcggc
     7261 ccgacttcgg tgtggaggat gatctcggaa ctcgacgatg atgcgctgct cgatttggcg
     7321 cacagcttcc gcgagggtgt gcccgtggcg acgccggtgt tcgacggcgc gcgcgaagcc
     7381 gatatccggc acctgctcga attcaccgag ctgcccgaga gcggcaagat cacgctctat
     7441 gacggcatga ccggcgacga attcgcgcag aacgtgacgg tcggctacat ctacatgctc
     7501 aagctctcgc acctagtgga cgacaagatt cacgctcgct ccatcgggcc gtattcgctg
     7561 attacgcagc agccgctcgg cggaaaagcg cagttcggcg gtcagcgctt cggcgaaatg
     7621 gaagtctggg cgctggaatc ctacggcgcg gcgcacattc tgcaggagct gctcaccgcg
     7681 aaatcggacg acgtgtatgg ccgcgcgaag atttacgaag ccatcgtgaa gggcgaaccg
     7741 ggaatcgagc ccggcgtgcc cgaatcgttc aacgtgctgg tccgcgagct gcagtcgctc
     7801 tgcctggacg tggaactgat gaagcggcag aagccgcagg ccgagcagtc cgaggcggct
     7861 gactaatatc gcgtcccggt gagtagccgc tgcaactcac cgcggacgcc acgagattct
     7921 ggaaggacag tttttgctcc ggcgtagccg gagaggagga accgcccttg tatcgatcga
     7981 gcccgtacga ccgagccagt cagatcgcgg attttgattc gattcgcatc agcctggcga
     8041 gtcctgaaaa aatccggtcc tggtcgcatg gagaagtcac caagccggag acgatcaact
     8101 atcgcacctt taagccggag cgcgacggcc tgttctgcgc caagattttc ggccccgtga
     8161 ccgactggga atgcctctgc ggaaagtaca agcggatgaa gcaccgcgga gtgatctgcg
     8221 ataaatgcgg tgtggaagtg acgctgagca aggtccggcg cgagcggctc gggcacatcg
     8281 agctcgcttc tccgtgttcg cacgtctggt ttttcaaggg gctgccgagc cgcatcggct
     8341 acctgctgga catcacgatg cgcgatctcg agcacattct ctatttcgag acgttcgtgt
     8401 gcgtcgatcc cggtgaagtt ccgggcatta aggaaaagga catcctgccg gaagaaaagt
     8461 tccgcgaatt gcagcgggac aaccccggaa aattcgacgc gcgcatgggc gccgaagcga
     8521 tcaaggaact cctgcggcgc gtggacgtcg acaagctttc gggcgaactc cgcgagaaga
     8581 tgaaggtcga tccgagcctg cagaagcgga tcaagtatgc gaagcgcctg aaagtgctcg
     8641 aagcgttccg caagagcggc aataaacccg agtggatgat tctggacgtg attccggtgc
     8701 ttccgccgga gctgcgcccg ctggtgccgc tggacggcgg ccgcttcgcc acatccgact
     8761 tgaacgatct ctaccgccgc gtgatcaacc gcaacaaccg gttgaagaag ttgatggaac
     8821 tgcacgcgcc ggacgtgatc gtccgtaacg aaaagcgcat gctgcaggaa gccgtcgacg
     8881 cgctgttcga caacggccgc cgcggccgcg tgctgcgcgg aaccaacaac cgcccgctca
     8941 agtcgctttc cgacacgctc aagggcaagc agggccgctt ccgccagaac ctgctcggca
     9001 agcgtgtgga ctactcgggc cgctccgtta tcgtcgtcgg tcccgaactg aaactgcacc
     9061 agtgcggctt gccgaagaag atggcgctcg agcttttcaa gccgttcatc tatcaccgtc
     9121 ttgaagccgc gggccactgc accacgatca agcaggcgaa ggagctggtc gaggcgcagg
     9181 aatcgaccgt gtgggacatt ctcgaggacg tgatccgcga gcatccggta ctgctcaacc
     9241 gcgctcccac gcttcaccgc ctcggcattc aggcgtttga gccgacgctg gtggaaggca
     9301 aagcgatccg catccatccg ctggtctgca cggcgttcaa cgccgatttc gacggcgacc
     9361 agatggccgt gcacattccg ctttcgccgg aagcgcaggt cgaagcgtcg gtgctgatgc
     9421 tcagctcgca caacatcctc tcgccggcga acggctaccc gctcgcagtt ccgacgcagg
     9481 atatggtgct cggcatctac tacatgacca aggcaaagcc gaaggcgaag ggcgagggcc
     9541 gcgcgttcgg tacggttgaa gaagtgatca tggcgctgga ggcaggcgag gtagagctgc
     9601 tcacaccgat ccgcctgcgc tacacgggcg aagtgatcga tctcacgaac gcctacgacg
     9661 atcaggacgt gtcgcacacg gagccggtgc agctcaacaa gcagtttctg cacacgaccg
     9721 cgggccgcgt gatcttcaac gatcacctgc cgtcggacat gccgttcatc aacggcttgc
     9781 tcaaaaagaa gggcattcag gccctcgtgc agtacggcta tctgcgctac ggcctcgaga
     9841 agaccgtcca gatgctcgac cggctgaagg agctcggttt cctttacgcc accaaggccg
     9901 gcgtctcgat cggcatcgtc gacatggtca tcccgggtgt gaaatccaag ctcgtggata
     9961 cggccgagca cgaggtcgtc aaggtgcagc aacagtacct cgacggcgca atcaccaacg
    10021 gcgagcgcta caacaaggtc atcgcgatct ggagcgacgt caccgaaaag gtcgcagatg
    10081 aaatgttcaa ggccctcgag cagcaggata aagagggcgt gatcaacccg atttacgtga
    10141 tggccgactc gggcgcgcgc ggatcgaagc agcagatccg tcagctttcg ggaatgcgcg
    10201 gattgatggc caagccgtca ggcgaagtca tcgagacgcc gatcacgtcg aacttccgcg
    10261 aagggttgac cgtgttgcag tacttcatct cgacgcacgg cgcccgcaag ggtctcgcgg
    10321 atacggcgct caagaccgct gattccggct acctgacgcg gcgattagtc gacgtggcgc
    10381 aggacgtgat catcaatgag ttcgactgcg gcacggccga cggcatctac gtcgaaccga
    10441 ttctcgaagc cggcgtcgaa gtcgagccgt tgcgcgaccg cgtcgtcggc cgcgtttcgc
    10501 tcgagaagat caaggactac gaaggcaacg tgatcgtcga gatcaaccag gagatcacgg
    10561 aagagctggc gaacgcgatt caggcggcgg gcatcgagcg ggtgaagatc cgctccgtgc
    10621 tgacgtgcga atcgcggcgc ggcgtgtgcg cgcgctgcta cggccgcaac ctggccaccg
    10681 ggcgcttcgt cgagatgggc gaggcagtcg gcgtgatcgc cgcgcagtcg atcggcgagc
    10741 cgggcacgca gctgacgatg cggacgttcc acatcggcgg cacggccact cgtgtcacgg
    10801 aacagtcgaa ggtcgaagcc cgcaacaacg gcttcgtgaa attcatcgac ttgaaggcgg
    10861 tcgagggccg cggcaagacg ctcatcgcca tgaaccggtc cggcttgatc gcggtcgtcg
    10921 atgaaaaggg ccgcgagaag gaacgccacc acgtggtgta cggtgctcgg ttgcgcgtcg
    10981 aagacgggca gccggtcacg ctcggacaga cgatgtgtga gtgggatccg tacacgttct
    11041 cgattctcac ggaaaccggc ggcacgatcg aattcaagga tttgcagccg ggcctcacga
    11101 tcgaggagca ggtggacgaa gtcaccggcc tttcgcagct cgtggtgacg ctgccgccgg
    11161 gcgacgaaaa gcaccagccg acgattcagg tccgcgactc ggcgggacgc atccgcaagc
    11221 gctacctgat gccctcgcgc gcccacttga tggtcgccga tggcgatgat gtgcaacccg
    11281 gagacgtgct cgcgaagatt cctcgcgaaa ccacgaagac gaaagacatc acgggcggtc
    11341 tgccgcgcgt ggtcgaactg tttgaaactc gcaagccgcg cgatccggcg gttatcagcg
    11401 agatcgacgg tgtcgtaaag tacggcgaga tcgccaaggg gcagcgcaag atctacgtcg
    11461 aaagcgagga tggcaggacg caaaaggaat actccatccc gcgcggcgtt cacatcaacg
    11521 tgcaggaagg cgagcacgtc cgggcgggcg agccgctcat cgacgggccg ctcaatccgc
    11581 acgacctgct cgccgtgctc ggtgaaaagt acacgcaggc ctacctggtg aattcgatcc
    11641 aggaggtcta ccggctccag ggcgtcaaca tcaacgacaa gcacatcgag acgatcgtcc
    11701 ggcagatgat gcgctgggtg aaagtcgaag atgtgggcga tacgtcgttc ctccttgagg
    11761 agcaggtgga caagttccgc ttccgcgagg aaaacgacca cgtgattgcc gatggcggca
    11821 ggcctgcgac cggtcgtccg cttctcctcg gaatcacgaa ggcgtcgctc tccaccgatt
    11881 ccttcatctc ggcggcgagc ttccaggaga ccacgcgcgt cctcaccgag gcggcgattt
    11941 ccgggaaggt cgattacctg cgcggtctga aggaaaacgt gatcatggga cgcctgattc
    12001 cggcgggcac gggcctggag cgttatcgca acatccaact cctcacccaa ttgccgccgc
    12061 cggaagaggc cgaagccgga ttcggcgccg gcttgccggg cggtccggag ctatcgccgg
    12121 aagaagctgc ggcgctcgat ttcctgcgca aggacacgga cgcggcggcc gaaggatagc
    12181 cacgtttcag acttatgggg cgggcagtcg cgaaagcgag cccgcccttt tttcttttct
    12241 gtcacggaag tatgcccgcc gtcggctcgc gtggttcccg gctaaggctc acgaatcgcc
    12301 tgctttgccc agtaagcagt ttggcatcaa ggcacgtttt ccttctctgt ggctaccgtt
    12361 gcgccgggta gaattttaca ggtgtttggg acgcaacccg ggtcaatacg aagccttccg
    12421 tactgcgagc aggggcagac gtagtcatgt ccaccgattc gtcgcaacgt cacccgaccg
    12481 aaccagagcc gccggcgcca gatgaaagct tggcggaggt aattgcccgc tctcagcaaa
    12541 actaccgcga gctgatcgat cacctcgacc aggcactctt cacgctttcg ctcacgggtg
    12601 aaattcgcgt tgccaacctt cgactcgccg aaattctcgg cgtcaggttt cccgagctga
    12661 tcggccattc gctcggtgag tttgtcgagt cgccgagcct cgcggacgcg gaacgcgccc
    12721 ttccggcgct gatggagcgc ggtacatggt cgggcacggt cgcggtccga ctgaaaaacg
    12781 aaaaggaaga ccgccatttt tcgtgctggc tgcagaccgt tacggagaat gggcaggtcg
    12841 tctccgttac aggctgggcg cgcgatgtta gcaaggagta cgaagcgcaa gcccgtttct
    12901 cggagctgtt cgaatcgctg cgcgaaggaa ttgtgttttc gacgcccgaa gggaagttgg
    12961 tcgacgcaaa tccggcgctg gtgcgactcc tcggctatga aagtaaggaa gaactgaaac
    13021 gcctcggcat gcgcaatctc tacgctgacc ccgccgggcg cgaagctctc atgaaggagc
    13081 tgactgagaa aggcgaggtg cacgactacg aaatcgtgtt gcgccgtaag gacgggaaga
    13141 agctgcaatg cctcacgtcg ggctttgcga ttcgcgatgc gtccgggcgc ccggtgcgga
    13201 tgcatggaac ggtcgtcgac atcaccgaac gcagggaaat cgagaggcgc ctgcatgagg
    13261 agcaggagtt cgcccgcaag ctcgtggagt gctttccgca cctcatcgtc gtgctcgatc
    13321 gcaaggggcg ctttacgttc gtgagcgacc gcatacggga cgttctcggc gtgtctccgg
    13381 aagaatacat ggggaagtcg gtcggtcagc gaaccgatga gggcgaccaa cggaaactgg
    13441 ctcagatgca ccaggacatc gtttccgggc ggaagagcca ggttcaaatt gaaattcgcg
    13501 cccgccacgc ggacgggacc tggcggtcgg tgcaggtcaa tgcgagcccg ctcttcgacg
    13561 aacatggcca gatcagcggc gtggtttcgt ccggccagga tgtgacggag tcgaggagga
    13621 tcgagcagca gctcgcgcag aaggaaaagt tcacggcaat ggggcagatg atggtcggcg
    13681 ctgcccacga actgaataat ccgctgacgg ccattctcgg cgtaaccgat ctgcttcgcg
    13741 agcgtgccac cgacgacacg acccgccggc aagtggagct tgtcttgaag caggcgcgga
    13801 gggccgcaac gatcgtacag aatcttttgg cgttctcgcg cccggtcacg ctcggcagga
    13861 cccgactgaa cctgggtgac gtggtgaaag aagtgctgct gcttgaagcg gccacgcttg
    13921 accagaaaaa gatccgcgta acgtttgacg cgccgagaga tcttccgccg atcgagggag
    13981 accgcaaact tttgaaggag gttttcctca acatcatcgc gaacgcggag caggccatgc
    14041 ctcccgaccg cgaccatggg acgctggcaa tcaagttatc gcgtgtggga aatcaggtcc
    14101 gcgtgagttt taccgacgat ggcatcggca ttccgcctga aagcatgggc aagatcttcg
    14161 atccattctt cacgaccaag cgcccgggtg gcggaagcgg tctcggcctt acgatttgcc
    14221 tggcggtggt caaggaacac ggaggaagga tcgaggcgga atctgctccg ggatcgggcg
    14281 cggcgttcca cgtctttttt ccggtcctcg ccgaaagctc tccagaggcg ccggactcgg
    14341 gttcggccgc cgcgggcggc gccgttcgtc aaaagccgct tccagccggc tcagaagtcg
    14401 tgcagggcca ttccgttctc gtcgtggacg atgaagagag catccgcgaa atcattcaag
    14461 aggggcttgt tgcgcgggga atgaaagtgg atgctgctgc gaactcggaa gacgcacttg
    14521 cttacctggc gaaaaattcg tgcgacgtgg ttctgtgcga tttcaatctt ccgggtatga
    14581 gcggcgaaaa gtttttcgaa agtttgcgct cgcagggcgc ggaaaagctg ccgcggtttg
    14641 tcttcatgac aggcgaactg ctcgaccccg atgccctcga gcgctaccgc agcaggggtg
    14701 cagccgtcct tcaaaagccg tttcacgtat ctgcgctggt ccagcttttg ggcgaaattc
    14761 tgaaggcgca gcccgcgcag gtcggctagt tcggcactgg tgttagttgg ctttgtgtcc
    14821 ggagactgca cctgcatcta cgccgaaatc ccagacgtag aggctcatac cttcttcgtc
    14881 gagcacttca gcaaacgcgt actcgcccgg ctcaagtggc ttatcgagcg tgaatcggta
    14941 gacgccttga gccacgggcc atttctgcat cggcagggaa tcgcgaagtt ctgccttttc
    15001 gccgaacagt tgatcgatgt gttcaatcag acggctatct ccatgcgcct tggcgcggat
    15061 cagctcgaga cgcggttcgc gggcgtctgc ggtgcggatg taaaattccg gctggctgac
    15121 ctgcagccgg aatttggcgc gcgcgccctg aatcgatacg ttttgccgcg aaggaacgat
    15181 gggaatcggc accatcacct gctcgagcag acgtcctttc gagatcttcg agcttgtttc
    15241 cgcctgagga agcgggagga tggatttgcc gtcgaaagcg aaaagattga cgcctggcgg
    15301 caagaacacg ccgggcgcca cttcgaggct cgcatcgata tcgagcggtg gtatgacgcg
    15361 agcggcttcc tgcgcttcca ccttggcggc gagtgtggca tcccgccgag cctggtcggc
    15421 ctcggccttc ttggtcgcgt cccaatcgac gagatcaact ggcatcgcct cccaggacga
    15481 gctgtcgagg ctgtagtagc ggacgcgatc gccgtcgatc ctgtattcgc gtacgagctg
    15541 gtagctgccg tccttcagca gaagcttcct accgcgcggg agaacgcggg acgaggaccc
    15601 gtttgaagtc tgactagcgg aagaggaatc tgccgggcta gacggtgcct gctgggcgcg
    15661 caagggcacc attgaaagga cgatcgagag gggaagaaag aacaccttca gacccgatcg
    15721 gcctagggaa tatcttgggc tggggcgccg gcgcgccatg ctttttccgt cctgatatag
    15781 tgcgatgccc gacgagggca tttggttgag ccaatctcag catacgccgg ggtacacggg
    15841 tccacaaccc gtgccacgga ttcagtaagt gaaaccctct gtcagagtga attgggacga
    15901 cggctgatga atcgcccggg ggtgcaggcc tagtgctcgc agcgagggtg caggacggcc
    15961 atgatcatca ggtccgaatt cgtgaagagc cacttctgaa cgttcgagcc ggccgcggcg
    16021 gccattgatc cgaggtgcct ggtttgtacg aggccgcgaa ggtgctcggc gcaaatgccg
    16081 gtcaggctga tgacctcttc ctcagtgaag aatttctggc tcgacttgat tcggaccatt
    16141 tttccggcct cacgaaatct cggatgctga agtcagacta atcgctgcct aattccccag
    16201 caatagtcgt tccgtaccag gcggatagaa ctaaatgctt ccgtgaaagc cgaaaatcgc
    16261 gaaaaacccc gcatttaggg gcggctttac acctctcgga ctttcgcacg gcgaacctgg
    16321 ggaacaccag agccggcaaa aagattctat cttgggaata ttgctaccac ttcacggagg
    16381 tgtttcgcgt gtcgaaatac tgtttcgacg cgcggtgcga actctgactg gcgcggttaa
    16441 ttggatcaga cggaaaagcc accggcggat tcgaaggctc gagcgatccg gaagagggag
    16501 ccctcgctga agtggggcgc gaaaatctgc attccgacag ggagtcccgc cttggttttg
    16561 ccgcagggaa cggagatgcc cggaacgccc gcgagcgatc ccgtcacggt gtagatgtcg
    16621 gccaaataca tctgcagcgg atcggccgat ttttccccta tcttaaaggc gggagtgggt
    16681 gcagtcggcg tgacaatcac gtcgactttt tggaatgcgt cggtgaaatc gccggcgatc
    16741 agcgaccgaa cacgctgtgc gcgcaggtag taagcgtcgt agtagccgga ggagagcaca
    16801 tacgttccga gcatgatccg gcgcttaacc tcgggcccga aaccgccttc gcgtgtattg
    16861 cggtacattc cggtaagcgt gccgccgggc acgcgcagac cgtagcgcac cccgtcgtag
    16921 cgtgcgaggt ttgagctggc ttccgcggtg gccaggatgt agtaagtggc gatggcataa
    16981 tcggtgtgcg gcatctgcag ggggatcctg cggcaaccga gtcgctcgag cagcgcaatc
    17041 cccgcctgga ttctttcctt cacctcggag tccaagcctg caccgaagta atcctcggga
    17101 acaccgatcc gaagcccttg gaccggcttt tccacttctg ccgcataatt cggcacgagc
    17161 acatccgccg aggtcgagtc gctggcatcg tggccggcga tcacgccgag gaccgttgcg
    17221 gcgtcggcga cattggctgc gaacggaccg atcttatcga gcgaggaggc aaaggccacg
    17281 agcccatagc gcgagacgcg gccataggtc ggcattaatc ccgcaattcc gcaaaacgac
    17341 gcgggttggc gaatggagcc gccggtatcc gttccgagag ccgctacggc cagacccgct
    17401 gcgacggctg cggccgagcc gccgctggag ccgccgggaa cgcggtcgag cgcggccgga
    17461 ttccggaccg gcccgtaggc ggaattttcg ttcgagccac ccatcgcgaa ctcgtcgcaa
    17521 ttggttttgc cgaggaacac ggcaccagcc ttgtcgagcc gctcgacaga ggttgcgtcg
    17581 tacggcggga cgtaatgctc gagcattttg gaggcgcacg tggtgcggag accgcgcgtg
    17641 ctgatgacgt ccttgatcgc aattggcact cccgccaggg gaggcaaagt ttctcccctc
    17701 gcgatcgcgg catcgattcg atcggcctga gcgtaggcgc gctccggcga aacggttagg
    17761 aacgcgttca gctcgcgatt gcggcgctca atccggccga ggaattccgt ggcgagttcg
    17821 cgcgcggaga ttttcctggc ctcgagcgcg acgcgcacgc tcgcaaccgt ccacgaagcc
    17881 agcgcgctca tcggctattc accttccacc tcgccggtcg caatcaccct tgggacgcga
    17941 aagaagggag cttgcgcctc gggcgcgccc ttgagcacgt ggccagcgac atcgcaggga
    18001 acaagcacgt cttcgcgaag ttcgggatgt ttttcttcgc cgccggacgt cgctgcggga
    18061 tgtagaacct gcgccatcgg ctcgacgttc gaggtatcga gttccttgag tttgtcgaca
    18121 tacgtgagaa tcgcatcgag ctgctcgcga tagagctcga cctcggcgga cgaaagaccg
    18181 agatgcgcca atcccgcgac tttgatcaca tcttcgcggc taattttcat tcaacgccag
    18241 tctcccgtct ggtccacttt ttggcgatta ggaaattctt tggcctgtat tttcattggc
    18301 ttagctctgc tcctacttgc ggactttggc gattagaaaa gcagtgacaa gcgtcaagtg
    18361 gtgagtggct agtgaagggc ctcaccgaaa ggcctcgcgc gagtgccgag tagctgacgc
    18421 agcggtgctt cctttcgatt agattgcggc gatttctact ccgcggaggt gacgagcaga
    18481 attgcggccc gtcgacgcgg gctggccggc gcaattcgat gaaccggctt aggccaatcg
    18541 gcgacttcgg cacttcgttt cccatttacc gcgtcgtccc tttcgcgcgc cgcattcgtc
    18601 tttggcgctt cagtctaaac gctagcacga tgtcaagcga ttttgcgcgg ggaagtctgt
    18661 ggacttcctt cagtctcgcc ttatcgagga tacgctagga gcggcgacgg atgaaggggg
    18721 tgtggtcgtt gtcggcttcg tgcagaaggc gcttcgcggc ggttcttgct gccagaaatt
    18781 tcacctcgtg gacgaggttt ccgccccagg cgcggttgat tcgcccgcag agttcgcgct
    18841 gcatcggttc gagctgctgc cgccacgctt tgccgtcgac cgcgagatcg agacgacctg
    18901 caatgcaacg cgtcggccgt gtgtgggccg cgagcgctgg accgacgatc gaaggccaag
    18961 cactcgacag ccacgcgagt ccggcttcag gacggtcgag gcgccgcaat gctttgccca
    19021 aaatgtcgcc cgctctctcc atggcgtggc gattctagca tgaacgcaga ttggcatttt
    19081 cacttgtgag aggagcaggg acctatcgtc gctcctcaca gaaagcgagt gcacgagcga
    19141 cgcggcgggc ttcttgcgcg ggcaaatcaa gcatgcgaaa atcatgactg ctgattggtg
    19201 gaatccgcgc aatcattttc tttttctctt caagcggcga tatcgtctaa gggttaggac
    19261 gtggccctct caaggccaaa atccgggttc gaatcccggt atcgctacca agctttctta
    19321 agcctctatt cctctcaatg ccgtaaacta gcccaagatc aacgatgttg atcccgatct
    19381 cgggatcatc cacttctcgg agtgtgtcgc gaacttgctc ttccgtcgga agggccatga
    19441 tggcacctcc tgtcaatcgc ccgatttgtt taccgagccc tttctcacat gcccaagaat
    19501 cctacttcgg agaacagagg tggcaagtga tcaccgtcac gccaagacca aatcacggcg
    19561 gattatagtc gcttcctcgt ggtcacgcac aaccgggcac tctcgccaaa tgatcccgaa
    19621 ataatgtact ttgctcgcgg gtcacatcaa gaacggaggg agagttccgt gagagccgaa
    19681 ttccccaagt ggacaaagca ttttcaaaga agctatccga aggtctggaa ggcctttcaa
    19741 aagttgggcg aggaatgcca cagcgcgggt ccgttggacg cgcgtacgcg gcgcttggta
    19801 aaagtcggaa ttgccgcggg gtatggctcc gaaggcgctg tgcactccgc ggttcggaac
    19861 gctttggctg aaggagtctc ggaagacgag atacgacacg tgatcttgct gtccattacc
    19921 accattggat ttcccaatgg ccaggcggcg ctgagttggg cggaagacat cgtgcgcgcg
    19981 aaatagctga cggttgatag gagtgcccat agtctcctac ctgtaagtca tctggcacga
    20041 gcgcgcttcg atcgaaggcg aacgttgctt ttccgagcca ccgcggcggc catatcggcc
    20101 agcgtggtat cttgcaggta gttcatcacg tgtgttcgca gcgccttcca ggtctcgtgc
    20161 agcgggcaaa gggattcgtc cgagcagtgc tccagaccca ctgcgcattc ggcgaactga
    20221 tccaggccgt cgatcgctgc gacgacatcg cgcaatgtga ttagctgcgg gtctcgcgcc
    20281 aatcgaaatc caccgccggg tccgcggcgc gactccagca gccccttacg aaccaactcc
    20341 tgcaatacct tgcccaagat gggcgccggg acgccttctc cctcggcgat ttccgcccct
    20401 tgtaccgccc gacggtcggg gaatcgagtc aggaagacga gggcgcgcac cgcgtattca
    20461 cagggctttg aatagatcat agtggaatac ggactctatt ttccttatat cgttctggaa
    20521 cgtcaaggct tgcgatcaat ttgggcagtg gaggtgtgac ctgtatcact ccgcagcgtg
    20581 acggccttca cagctagcct tgccaggcga agggtatgcg atatggggaa gtcgagcgtc
    20641 cgccgtggtt gggcctagtc tggagagaca ctccgaaggg aagggcaatc aggctgtgga
    20701 aagttctatt gacaagcgag cgaaatacga aataaaaggt atttaattcc tgtatagagg
    20761 attcgatgct atacggacac gcggccgaga ttgcaattcg agcgggcctt tatctcgcgg
    20821 gccggccgcc gggggaactc tccccggttc acgaagttgc caaagctgcc aagctcccga
    20881 gtccatatct ctccaaggta atccagaaac tggcccaagc gggtctcgtc cggacgtttc
    20941 ggggaccggg cggcggtatc gaactcggaa aggctcccga ggcgatcact ctgtggtcct
    21001 tggtgtgtgc aacagagggt ccagctgatg tgaatcggtg cgcactgggt attggcgact
    21061 gctcaccgag taagccctgc cctctgcacg gcaggtgggc gccgttgcga gacgagttcc
    21121 aaagactgct ggaagggacg actcttgcgg cgctcctgcg agaccttgca gagacgcaag
    21181 gccaacaagg atcgattcgg agactcatag ccgagaaaag cggcccaacg ccttcccgcg
    21241 aaatcgcccc tgaaaggcag gtacgccgat gacatccgtt cgtatcgtac gaattgggat
    21301 tctgctggcc ggcatgtgcg cgtgcttggc caggccatcg gtggccgatg tccaagacgc
    21361 aggcagtttt ttccgacaga actgctcggc gtgccacacg atcggaggag gccacatcat
    21421 cggccccgat ctacaaggtg taacgaaacg caaggatcgc gcctggctgg tcaagttcct
    21481 tcagagcccc aaggccatga tcgacagcgg cgacccgtac gccgggcagc tggtgcagga
    21541 cgcgcccggc cacatggtga tgccgacggt ccccggtatg aacccggatt tggccaatgc
    21601 tttgctcgac tacatcgaag gagggactgc ttcggcaaaa cctcaggctg ccggccccgc
    21661 tatcagcgat aggccattta ctgttgccga cgttgcgctc ggacagcagc tattccttgg
    21721 cgaacgaccc ttcgaaaatg gcggtccggc ctgcgtctcc tgccacacgc ttgggacgat
    21781 cagcggattg ggaggcgggc gacttggtcc agatctgacc aacgtgtttg accggctcgg
    21841 cgggcgtcag ggtctggggg catggctgtc tacaccgccg acgccgacga tgcagtccgt
    21901 cttcggcaaa catgcccttc aaccggaaga gatttttcct ctgcttgccg tgatcgatga
    21961 tgcaaatgct cgaagtcagc cagcgaatca gtcgtcgatt ctgaagtttt tcgcctttgg
    22021 tttcggcgga acgcttgttg gtctcgctct gcttcagatt gcttggcgag gacgaatcca
    22081 gtctgttcgt cgatccgttg tccgaacaca cgcgcgaggt gcgcaatgaa acacaaagac
    22141 gctctctcgt ggattcgcga cgagtccacg ccgcaactca gaggatggga ggagttctat
    22201 cgcaatcgct ggcagcacga caagatcgtg cgcagcacac acggcgtgaa ctgcaccggc
    22261 ggctgcacct ggcagatcta cgtgaaagac ggaatcgtga cctgggagat gcagggcctc
    22321 gattatccgt cgctcgaagc cggcttgcct ccttatgaac cgcggggctg ccagcgtggc
    22381 atttcctact cctggtatct ctacagccct ttgcgcgtga agcacccgta catgcgtggc
    22441 gcgcttatcg atctgtggag agaagcgcgc gcgagctacg gagatccggt ggaagcgtgg
    22501 gcgtcgctcg tcgaaaatcc cgaggcgcgc aagcgctggc aacaggcgcg aggcaaggga
    22561 ggctttcggc gcgctgattg ggctacggct ctcgaattga tcgcggcgtc ctgcgtttac
    22621 acggccaaga aacacggccc tgaccgcatc aacggatttt caccgattcc ggccatgtcg
    22681 atgatcagct acgccgcagg ttcccgcttt ctgcaattgc tgggcggcgt ttcgctctcc
    22741 ttttacgact ggtattgcga cctccccagc gcttcaccgg aaacctgggg agaacagacc
    22801 gacgttcacg agtcggccga ctggtacaac gcaaagctgc ttgcggtgat gggttcgaac
    22861 ctcaacatga cgcggacccc cgattgccac ttcgccgccg agtcgcgtca caacggcacg
    22921 aagatgtggg tcttctcacc agacttcagc caggtatcga agtacgccga cgaatgggtg
    22981 gccatcaatg ctggacagga cggtgcgtgg tggatggccg tgaatcacgt cctgctgaaa
    23041 gaagctcacc accagaagca gactccctac tttctcgact acgcgaagag gtacacggat
    23101 tctccgtacc ttgtcgaact gcatgagaag ggcggtgttt tccagcccgg ccagcttctc
    23161 cgcgccaatc ggctcggggc atattcgggc gtcgaaaacg gcgactggaa attcctgatg
    23221 tgggatgcga aggactcgcg ccccaaattg ccgatgggta gctccggatt caggtggggc
    23281 aaggaaaaag gaaagtggaa cttactgctg aaggacggtg tggatggcag cgagatcgat
    23341 ccggttctca ctttcctgca gtcgaacgac ggagtctccc aagtacgctt cgatgatttc
    23401 ggggaaggcc gtgcggttca gcgcggcgtc cccactaaga agattcggac ggccgatggc
    23461 aggaccgcga cggtcgcaac cgtatacgac ctgctgatgg cgcagtacgg cgtctcacgc
    23521 ggattgcccg gcgattaccc gagagactac aacgacgatt cgcagcccta cacacctgca
    23581 tggtcggaga agtacacggg catgagccgc gacatggtga tccgcttcgc gcgcgaatgg
    23641 gccacgacag ccgaactcac gaagggcaag tgcacggtca tcatcggcgc gggagtgaat
    23701 cactggtatc acgcgaacct gatgtaccgc gcggcgattc acgcgctgat gttctgcggt
    23761 tgcgttggcg tgaacggcgg cggcctggct cattacgtcg gtcaggaaaa gctcgcgccg
    23821 atggagccgt ggtcggcgat cgctttcgca cgagactggt tcccggcctc ccgccttcag
    23881 aacgccccga gctggcatta catccacacg gaccagtggc gctacgaaaa gagcttcacc
    23941 gattaccaca cggtgccagc gaatcagcaa gagggttctc ttgccagcgg gcacacggcc
    24001 gatacgcagg tgcgtgccgt gcgcaacggc tggctgccct tctatccgca attcaatgcg
    24061 aaccctctcg acgttgtgaa gcaggctgaa agcgccggag caaagaatga agctgagatc
    24121 tactcgcacg tcgtacgcca gcttcgtgat ggcaaattga agttttccgt cgaggatccc
    24181 gatgcaccag agaactggcc gcgtgtgtgg ttcatctggc gcggcaatgc tctgatggcc
    24241 agcgccaagg ggcacgagtt ttttctgaag cactacctcg gcacacacac caacgccatc
    24301 gcccaggaca tcgccgccga ttcagtccaa gagatcgcgt ggcacgagac agcgcctgtc
    24361 ggaaagatgg acctgatcgt tgatttgaac ttccggatgg acacctccgc tctgtattcg
    24421 gacatcgtgc ttcctgccgc gacctggtac gaaaaggccg acctcaactc caccgacatg
    24481 cacagcttca ttcatccgct ttcggcggcg gtgccgccgt gctgggaatc gaagagcgat
    24541 tggcaaatct tcgaagccgt cgcgaaaagt ttctcagagc tcgcgcagca gcacttcccg
    24601 aagccggttt gggacttggt ggcttcaccg ctcgcgcacg atacgcctgc cgagatcgcg
    24661 cagccaacga ttgcggattg gaaagaggga ggtatcgagc cggttcctgg caagtcgatg
    24721 ccggggctga agctcgtggc gcgcgactat tcgaatctgt acaaccagtt catttcgctt
    24781 ggcccgtctg tgcgcgcaaa tggcttgggc gcccatggca cgcattatcc cgttgacgac
    24841 acatacgatg aggccctgcg aacgcttccg acggaggaat ggggcggcaa acgctacccg
    24901 tcgctggccg cggatgagga cgcctgcaat ctgattctgc ggttcgctac ggtgacgaat
    24961 ggcgaactgg cttaccgctc gtacaaggac atcgaagaga aggttggcct tcccctcacg
    25021 cgtctggcgg aaaagagccg gtcggttcgc gtggattaca aagggttgca ggcccgcccg
    25081 cagcgcctgt tgaacagccc gatgtggtcc ggtctggttg aggacggccg cgcgtattcg
    25141 cccttcacat ataacgtcga gtgtctcgtg ccatggcgga cgctcacggg ccgacagcac
    25201 ttctacctcg atcacgaagg ctatctgcag ttcggcgagc acctgcccac gtacaaaccg
    25261 aaaccgtcgc ccgcgcaata cgcggacctg cgcctgagtc aggaagcagg accgacaaag
    25321 atgttgaatt acctgacccc gcatgggaag tggcacatcc actcgaccta cggcgacaac
    25381 caccgcatga ccacgctttc gcgcggggtt gagccgctgt ggatgaacga caaggacgcc
    25441 gctgatatca acatcgcgga caacgattgg gtcgaggtac acaacgataa cggcgtggtg
    25501 gtgactcgcg ccgccgtcag cgcgcgcatc ccgcgcggaa tctgcatcca gtaccactcg
    25561 ccggagcgca cgctttccgt ccctaaatcg ccgctgcgga aggggcgccg tgccgggggc
    25621 cacaacagcc tgacgcgcgt acgtctaaaa ccgaacttga tggtcggcgg ctatggccag
    25681 tttacgtacc acttcaacta ttgggggccg accggctgca accgcgacac tcatgttttc
    25741 gtgcgcaagc ttcccgaatt gcagtggtag gcaaaggagc catgcgatga acgtccgctc
    25801 tcaagtttcg atggtctttc atctcgacaa atgcattggc tgccacacgt gcagcatcgc
    25861 ttgcaagaac atctggaccg atcgcaaggg caccgaatac atgtggtgga acaatgttga
    25921 gacgaagccg ggaaccggct ttcccaccga ttgggaggat caggacagat accacggcgg
    25981 ctgggaactc catgacggca agcttcggct gaagtccacc ggcaaggcca agaccctatt
    26041 caacatctac cacaacccgt ctatgcccac gatggacgac tactacgagc cgtggaccta
    26101 caaatacgag gaattattca ccgctccggc gggaaccgac cagcccacag cgcggccggt
    26161 ctcgatggtg accggtgaac tgatcaatat cgaggccggg cccaactggg acgatgacct
    26221 gggcggctct ccgatctacg ccgcgagcga tcccaacctg aagggcctca accacgatac
    26281 gcagcaacag ctcttcgcga tcgagcggct ggtgtttttc tactttccgc gcatctgcaa
    26341 tcattgcctg aatccttcct gtgttgcgtc gtgcccttcg ggagcgctct ataagcgcgg
    26401 cgaagacggc atcgtgcttg tagaccagac gcgctgccgg ggctggcgcg cctgcgtggc
    26461 ggcatgcccg tacaagaaga tgttctacaa ctggaacacg ggcaagtcgg agaagtgcat
    26521 cctttgcttc ccgcggctgg agaccggtca ggctcccgcc tgcttccatt cctgcgtggg
    26581 ccgcattcgc tacttgggcg tcttgctcta tgacgcctcg cggatcgaag aagtggccag
    26641 cctgccgagc gaaaagctga tcgaaggcca gcgctcactc attcttgacc cgaacgatcc
    26701 cgaggtgatc tcggccgcac gcagaaacgg cgtacacgaa tcggtcctcg aatcggcgaa
    26761 gcgttcgcct gtctatcggt tcgtcaaaga ctggaagctc gcgcttccac cgcatttgga
    26821 ataccgcacc atgccgatgc tgttctacgt tccgccgatg tctccggtga tgtcgaaccg
    26881 atcgaacggc tcgctcgatc acgtttcgga tgatctcttt cacgacatcg aagaggcgcg
    26941 cgtgccgatg aagttcctcg cgaatctatt tggcgcgggt gaggaaggcc ctctgcgcta
    27001 tgcgttacgc aaacagaaag ccgtccgctg gtaccggcga ttcaagaccg ttggcgacgt
    27061 ggacgagcag accgccatgc gcatgctgcg cgaggcggat tgttcagtgg aagaggccga
    27121 ggccatctac cgactcacct ccctctgcac gtttgacgac cgcttcgtga tcccgccgat
    27181 gcaccgggaa gaagccattc agatgatgga agacccgctc gagcacaaac aaggcgtggg
    27241 attcggattc ctttctggcc cgaagagagg aatgtgatgg cggacgcgaa cgaattcgag
    27301 tcattggcag cgcttttgcg ctatccgacc gaagactacg cgcgcgaagt ggtccgctcc
    27361 tgcaaagtcc tggcaaacga cagtccggaa atcgacttgc tgctccaaca cttcgtcgaa
    27421 cagacacgca gtctctctct tgaggatttg caggtgcttt acacaacgac ttttgatctc
    27481 gatccggttt gttcgctcga aatcggctgg catctgttcg gggagaacta cgaacgcgga
    27541 gaattcctgg tgaggatgcg aggagagttg cggcggctgg gcgtgaagga gtccaccgag
    27601 ctaccggatc atctttcgca tgcgcttgaa gcgctcggcc ggatggaacc ggacgaagct
    27661 gccgattttg cggcggcatg cctgttccct gcgcttgaca agatggccgc gggaatcaag
    27721 gggaaatcga acccattcga aaacgtcctc agcgctgcta cccggttgct ggagcaccgt
    27781 tacccaaggc cggaacccgc agtggtcttg gcagaacctg catttcgcat tttggattcg
    27841 aaaggatggt gatgacatcc atgctgcctt tcgcaaacgg cctattcttc atggtgcttc
    27901 cgtatctcgc gatgttcgtc ttcttccttg gaaccatcat gcgctaccgc aaggctcctt
    27961 tcacgtattc gagtttctcg tcacagtttc tagaaaataa gcaacatttc tggggactcg
    28021 tgccgttcca ctacggcatt gtcgccgttc tgctcgggca tattgttgca ttcctgattc
    28081 cgcgcgagat tctggcgtgg aacagccggc cactgcgtct ttacgttctg gaagcgtccg
    28141 cgctggtgtt cggactgctg acgctggtcg gcctttgtgg cgcgattcat cgaagactga
    28201 ctttctcgaa ggtccgcgcg gttacaaccc cgatggactg gatcgtcttc acgctgctgc
    28261 ttgtgcaggt ggcgagcggg gtgtatgtcg cggtcttcca tccttggggt tcttcctggt
    28321 tcgctgcggc ggttgcaccg tatctctggt cgctcgtcaa gctcaaccca gatctcggct
    28381 tcgtcaccgc gatgccattg gtcgtgaaat ttcatatcgt gatggcatac accttgatcg
    28441 gggtggcgcc attcactcga ctcgtgcaca ttctcgtcgc gccgaacccg tacttctggc
    28501 ggaagaacca agtggtgcgc tggtatcgcg ctccagagga gatctcgtga gcggtctgcg
    28561 gcgcttatcg agaaggcttt ggagcgaagg tcggggccgc ggtgggatca gtgtggccgc
    28621 cgcaacggtt ctgttgtgct ctgcattagg ttggtcttcg gcggtgcaag ggcagtcaca
    28681 atctcagtcg caatccgacc cggcgcaggc gagcggttac aagctcggtc cgctggacat
    28741 ctccgggaat tggcgcgtgc gcgtggaggg gtgggactgg ttcgagggca actcgggaga
    28801 gaactcctac gcttttcctc attcgctgct gcgagttgcg atcgggcaac aaacggaacg
    28861 ctttgactgg caggtagagg ccgcccagga cgccatactt ttcctgccca ggcatgccag
    28921 cgtcgccgcg ccggcagtgc aggggcagct tgggccgggc ggcacttatt atgccgcgaa
    28981 cggaaacggt cgcaacaacg tcaacgcatt cgtaaaacag gcttacttcc agatcaatcg
    29041 tttgggcgag ggcaaactga aactggggcg tttcgagtat ttcgatggtt ccgaagtcaa
    29101 gccgaacgac gctacgctcg cctcgctcgt gcaaacgagg atctctcagc ggctgattgg
    29161 caacttcgct ttttcggccg tccagcgaag ctacgatggc gcacagttca gctacaactt
    29221 cgacaagaaa aacctgactc tgttcggcgc ccgaacaacg aggggtgtct atcagatcga
    29281 cggcatgggc gagctagacg tagatgtcta ctacggcgcc ctgaccttgc cgacatcgac
    29341 cgaacacagc gcgggcgagc tgcggttatt tggcttgggc tacatcgatc accgcacggg
    29401 cgtgctcaag acggacaacc gttcgccaga cgaccgcagg gcagaccacg gcgagattca
    29461 aatcgcgacc tacggcggag attacctcca cgtcttcaac acagacgcgt ccggcaagtt
    29521 cgacttcctt gtctggggcg cagttcagac cggctcgtgg gggcggctca cgcagcgcgc
    29581 gggcgctttc gtcggggaat tcggctggca gccgcgggtg aaaacactga agctatggct
    29641 gagtgctggc tattcgtacg gaagcggcga cggcaacggc agctccaaca actcagtcca
    29701 cgggacattc ttccaagtgc ttccaactcc gcggatctat gcgcgcatgc cgttctataa
    29761 catggagaac aacgaggact tctacggctc gctcaatctc cgaccccact cgaaacttcc
    29821 aatgcgcagc gagttgcacg cgttgcgcct cgccagccga aacgatctct ggtatctcgg
    29881 cggtggcgcc ttccagccgc aatcgttcgg ttacacggga cggcccagcg gcggcaatcg
    29941 gagtttcgcg aacgtttggg atctcagcgc ggactatcaa gtaacgcgga acttctctct
    30001 cggactctat tacgcgcacg cctggggtaa ggcggtgatc gcaagcattt atcccaaaga
    30061 tcccaacggc caatttgcgt acgtcgagac gaatcttcgc ttctgaagcg gtgggatcgt
    30121 ggtgggaggc agtacggagg agcaaaaaat ggactccata gtaggctcac ccaggaaggc
    30181 actctggagc gcgacgctgg ggttcttcgt tggttttgcc gcagtcgcac ttttcggacc
    30241 gtccgcgaac cgattccagc aagttatggg actcggcccc ctggctcttg gatttctcgt
    30301 ggccgtaccg tctctttcgg gttcgctttt gcggattccg ttctccgcgt gggtggacac
    30361 gaccggagga cgaaagccgt ttctctggct gctgagtctt gccgtagtcg gcatggtcgg
    30421 cctggcattg gtcgctcgtt ttctctatcc cacacacctc acgtacaaga gttacccgct
    30481 cctgatgacc ctcggcacgc taagcggctg cggaatcgca acgttctctg taggtatcag
    30541 ccaggtttcc tattggttcc cgcaacgaga acaggggcgc gccctcgctg tcttcgccgg
    30601 aataggcaac gtggctccgg gaatattcct gatgcttgtg ccctttgctt tgacccgatt
    30661 cggcctcgca gtctcctacc ttctctggct gtgcatcctt gtcgccggaa caattctcta
    30721 cgcgctcctc ggcaagaata gtccttattt tcagttgctg aagaagggcc tggtgcctgc
    30781 agatgccaga gctcgcgcac acgagcttgg acaggatctg ttcccgaccg gaaatctgaa
    30841 ggacagtctg cgaatctccg ctcgagtgtg gcggacctgg gcgctcgttt ggatctactt
    30901 cacaaccttc ggtggtttca tggcattgac cgcctggcta cccatctatt ggactcgctt
    30961 tttcggtgtg agcgttgagc acgccttgat gctgaccgca gtgttttcga tcgtcgcgtc
    31021 gttgattcgc gttgcgggcg gcgtactgtc cgatcaattg caggagggag gagagaacac
    31081 ggcgatcctg gcgctgttga tcactctcgt cggggcgctt gtgatgacaa gttcacgcca
    31141 gtttgagctt tcggttcctg gcgaggtcct aatggctttc ggcatgggca tctgtaacgc
    31201 ggccgtcttc aaaatcgtcc ctcaggccgt accaaaagca gtgggcggcg cagcgggatg
    31261 ggtcggcgga ttcggagcgt ttggcggttt cgtgattccg cccgtcatgg gatttgcggt
    31321 tcgcaatctt ggtcagagcg gctacgccat cggcttcatt acgttcatct ttctcgcgct
    31381 gttttctctt tccatggctt ggatactcaa atacgcgcgc gaggacgtgt cgccgcgcga
    31441 ttcaaccgtg cgggctaaag acgcggcggt cgccccggag actgcgcgag tgacccgata
    31501 aggagcggcg cggatgggtt ccgcgaaatc aaggtcggaa cgcggtaagg gcctctcgat
    31561 gggcaggtgt gtctgggcgg tgatcgccct gttcaccgtg cttttcggat gtagaagatc
    31621 gatcgaacaa agtgtctcgc agtatcgggt gcaggaactg ccgggaagtc taatcgagga
    31681 cacggttcga ttggagggaa cggcgccttc tcctaagaag attcatgtcg cgcaggacgt
    31741 cgatgtttgc ggggaaacgc gcgatgtgtt tctggtgcgc gtggataacg gcggtgtaga
    31801 cgatgccgtc gtctggatag acgatacgat cgtgactaca tcctttccgt cacactcaag
    31861 cgtcagaaca aggcaagaca gtacaggttc gccgtccaga aggactgtgc acgcgccaca
    31921 cccgcctagc tcgcatccgt gcttcgtccc catgaggttt agatcttcgc gaagcacctc
    31981 cagcaatgtc ttgtaaggag caaaggacac atgaacttct tcacggttta gcgtgaagcg
    32041 gatgtgggct gtctttcggc actcttcagg tattctctgc gtggtcatga ggtgctgcct
    32101 tcggtcgttc gtttacgact gaagcattaa aataggcttg aaaatgcaaa tagtgatagc
    32161 tgtcacaaat gattgtgacc gctatcacgc caagaaacag cccgatctcc ttgcgaaacg
    32221 ttctatttcg gtaggcggag gctgcgcggg tgtcgagagg aagcatgaag gtgtgacaga
    32281 agtcaccgat tcgatgctcc ttctaatcta tctttcggaa cgcgtatcgc tcttggaggc
    32341 cagggaatga gcacacttga ccagcgagta gaggctacct cgcggtggat tgcgagtccc
    32401 cggttcgccg gtatcgtccg cctatactcg gcgcgtgaag tggcagagca gcaaggcacg
    32461 atttccagcg actacaccgt cgcaagacaa gctgccgagg agctatacac gcgtctgcgc
    32521 gagctgttca agcagcgcaa acagatcacc accttcggcc cctactcccc aggacaggcc
    32581 gtgacgctca agcggatggg gatcgaagcc atctaccttg gcggctgggc gacttcagcc
    32641 aagggttcgt cgtccgaaga tccgggacct gacctcgcaa gctacccgct cagccaggta
    32701 cccgacgagg cagccgggct ggtcagagcg cttctcgcgg cggacaagaa ccaacacttc
    32761 gcacgcgcgc gcatgaccaa agagcagcgc agggcgactc cgacggttga ctatcggccg
    32821 tatatcattg cggacgccga cacggggcat ggtggggagg cgcacgtacg caacttgatc
    32881 cgtcgtttcg tggaagccgg tgttgcaggt taccacatcg aggaccagaa acccggagcg
    32941 aagaagtgcg gtcaccaaag cggaaaggtt ctggtcgcgg aggacgaaca gatcaggcgt
    33001 ctaaacgcag cacgcctgca gctggatatc atgcgggtgc cgggcatcat tgtggcccga
    33061 acggatgcag aagcggcgac atttctcgac aatcggagcg atgagcgaga ccagcccttc
    33121 atactgggcg cagccaatac cgaactgccc agctatcgga cgggctatct cgccattctg
    33181 aggaagctat ttgaactggg agtcgagcta cggggacacc ttcttttcaa tgtgtcggaa
    33241 accgagtatc aggcagcgtt tgcttggctg gatcgagtag ggctcgcttc gctaatcgcg
    33301 aaagttggcc cgggtcttgt gcatgcctca ggaatcgaac tcgacgaagc actcgacaag
    33361 atggacacac gctacatgga gatctggcaa gtagaagctg gactgaagac ctatggagag
    33421 gctgtcgccg acgtcatcaa gtctcggacg gacgaaggcg agcctttcga aacgaccgtg
    33481 gataagtggc ttgagtttgc gaaacgcgct tctttgtacg aggcgcacga acgggcaagg
    33541 tcaatgggca ttgaggttac ctgggattgt gagttgccca agacgccgga gggcttttat
    33601 caggttcagg gtggcattga ctatgccatc gccaagtcgc ttgccgcggc tcctttcgca
    33661 gacatcctgt ggatggaaac caagacagcg gatttggacg atgcccggac cttcgcagaa
    33721 gcgatccacg cgaaacatcc cgacaaaatg ctggcgtaca atttgtcccc ctcgttcaac
    33781 tgggacacca ctggcatgag tgaagaagag atgagaaggt tcccggagga actcggaaag
    33841 ctcgggtttg tattcaactt cattacctat ggtggccatc agatcgacgg catggctgcg
    33901 gaagagtttg cgaccgcgct gaaagaagac gggatgctgg cgctcgcgcg cttgcagcgc
    33961 aagttcagac tactggaatc cccctatagg acaccgcaga ctctagtcgg agggctgcga
    34021 cttgacggcg cactgatggc ttcgtcgggc cgcacggcgg caacgaaggc gatgggcaag
    34081 ggctcgaccc actaccagca cctcgttcaa acggaggttc ctactagggt gcttgaggag
    34141 tggctatcga agtggacgaa gcactggaag tatccggcga ggattcgagt cgagctgcgt
    34201 ccccacacgg ctggctcgga actgttggaa ctgaatgtag tcgacgccgt cagcaaggag
    34261 aagctcgcga gcatcatctt cgccaatgcc caggatcgtc gtggccggaa tttccttctg
    34321 gtccgcgatc agcagacagt ggcgcggcta cgtcaaaagc gggtcatgac gctcgtccag
    34381 cttttcctga tccatcgcta caaagctagc gcggtccact acctgacccc aacccaagat
    34441 aacctgttcc agacgcaggg aatgaagacc atcgggatct tctcagacgt gcataccgaa
    34501 gtcggtcaaa tcatcgttgc gcaagtggac aagctgcgtg tctccgaact gcttaaaccc
    34561 gatggaacag cgctttctga gctgatctgc cagaagtcgc cgtcggtggt caagcttgaa
    34621 gggaaagctc atcatcgctg atgaagaccc gcgacgagag ctccatgaac ggcattgtaa
    34681 ggtcgtgcgc gttccggaca aatttccggt ttgggctcaa ccgaatagac gcaagtcaat
    34741 ggtttgtgga tcgcggcaca gcgctacgct tcccactctc gatgagtgag tgtgtccggc
    34801 aaatgcgcta atcgggcgtc tatgaattca cttctgtgcc gcagatttcg attcctggcc
    34861 ttgggcccag agctggcgaa gccggcgcat ggcatttagt cccttcagct ccgtaaaatc
    34921 cttgagagtc tgaagcacga catggtcgtc cggaagggtt cgaaagtcat tccatggcgt
    34981 ccccatgatg gtgtcgaacc cgagaggcac tgcgagagtc aagaatgcgt tctctacaag
    35041 ctgccgcaga gcgatgccgt cgaacttcaa aggcggcagc atgttcccga tgttcgtaat
    35101 tccaccgatg atgtgaatac cctgcaagtc tggatcctgt cggatggtcc ggatggcgtc
    35161 cagcgccatt ttcgtgagcc cctccaggtc ggacacgaga ctatgaatgg tgacgtctac
    35221 gaaaaggtcc tgcggcgaga acccgcaatc cctcgtcagc ttgtccttca ggcggcgagc
    35281 cacgccgacc acttcgattg tcaacttgtt cgactttggc gctccgtctt caagtcgctc
    35341 cgaagccatc aggatgacct ggaatggtcg aatcttgacg agttcaagca tttccaggcg
    35401 agtctcagcg agtgagttca ccagcggctt ccgccctctg gccttggccg gatcgtaggc
    35461 tttcaggcaa accgcctgca cctccgcgct gggataatcg aagcaaagag gcacatccac
    35521 cgctgcctgc aatgcattga tcacttccgt caggaacttg aaatctttgt agcctcgtgg
    35581 cccgaccgtc acgtcaaggc accatgcccc cgcctgcacc tgctgcacgg ccaaagcctg
    35641 caagcccttg atgtcttcag cttcgacgag cgctttcgta gccttgaagc caggattgat
    35701 tcggtcgccg ataacgctga cacctggaat ccccatagtc gcctcctcct aattctttct
    35761 gcggtcggga aagcgcattg cgcgcatgaa cgctttgttg aagcgttccg ttcccgcaag
    35821 ctcgaaatac tggctgctcg ctgcgagggc ctgagcggtg gcgcggtgcg ggtacgacac
    35881 gagcgcgagc tttgctccga ttcccgcggc gttgcccacc tgcgcgaagc ggtccagcgg
    35941 caaatccggc aacatgccga ttgccagggc gctcgcaaga tcgatgtagt tgccaaaagc
    36001 cccggcgatg attacctgat cgatttcgtc ctcgcgcagt ccggcgtcct cgagcagcac
    36061 ctgaattccc gcgcgaatcg cgccctttgc gagctggacc gcgcgaacgt cgcgttgcgt
    36121 gaacaccacg gccttgccct tggtcgcgcc ttcgtcaacg agaatgaact ctgattgccc
    36181 atcgcgaacg cgcactcgcg ggtcttcttt tgtcattcga cccgtctgac tcacgatgcc
    36241 cgcgaaatag atttgcgctg ccgcgtcgat cactcccgat ccgcaaatcc caatcggggg
    36301 cgcgtttccg atcgtttgta tgcgcacgtc atcgccggat atcgcaaccg attcgatcgc
    36361 cccaggcgcc gacctcatgc cgcagagaat gtggcgccct tccagcgccg gtcccgaagg
    36421 gcaggatatc gtcagaagct gccccttgtg gacgagcgat atttcggtgt tcgttccaat
    36481 gtccaaagcc acgatggtct tttgctcgtg gtccgctccg atcgcgagca acatcgctgt
    36541 gtggtcgccg ccaacgaatc ccgcgatgtt cggaatcacg tgcgcgtacg cgccaggcgc
    36601 ggctttgatc ccgatgtcgc gggctttgac gtctgccgcg cttgcagccg ccgcgacaaa
    36661 gggaactgcg cccagataat ccacgggaag ctgcaacaac aggtgactca taaccgtatt
    36721 gcctgctacc acgacgtcgg cgatccgctc aggattgagg gattgcgact cgcacagctc
    36781 tttggccaca gcgttgattt cggctacgac cagatcgtgc agtttcttct ggttttccga
    36841 ggaagcccgc gccttgccaa tgcgggtaat cacatcggcg ccgtacttag tctgagggtt
    36901 ctcagtcgcg cggctcgcaa gggtccgacc gttgcgcaag tcaaccagga gcacgccgat
    36961 gttggtcgta ccgatatcca cggcgagccc cgggagcgga tctttcactg gcggagtcac
    37021 accgattact tcaccaaagc gaatcagcgc gacaacttgt ccgccattgg ctcgcaatac
    37081 tgcgggcagg aggcgcaaca cgtccaggtc gactctcgaa cacgtacccg gccacttctc
    37141 gttcagagca ttcatcagcc ggatgtcatc agcgatagga tgcgtcagcg ttggaggcct
    37201 aaccgtaacc gggtacagcc gaacggctgg atcgggacgg acccacacgt cctcgctttc
    37261 gacctccgtg cgcagcggca cggcgcgggc gcgctccgaa acctccacgc ggcaatcgcc
    37321 gctggcaaac gtctggcagg cccggcgcca attgttcgcg attgcctccg gcgaaaagaa
    37381 ttcgatatct tgcagggaag gcggctccac ggggccttcc gtcacccgaa cgacacatga
    37441 ttggcaaagt ccgcggcccc cgcacacgct ggcgatccgc acgccggcgc gatgcgcggt
    37501 atccaataga gtctcggtag ggagactcca aatctgctgt ccgtcgggtt cgaatgtgat
    37561 ggagtgactt gtccgagacg attcctgcgc ggatctcatg ccgcgacccc agacgtcctc
    37621 ggccagcggt gagggcatcg ctcccgggcc gcgcatcgct cgcaacgctc ggcgcgactt
    37681 tccttcggca ttcgaagccc gaaaccgacc acagccgaca ggctcttgcg cggcatgagc
    37741 atgccgccgc tcgtcagcga aacgccaatg tcggcgcccg ccgcgagctc caggactacg
    37801 gcttgttgcg gaatggcgaa gccgtcgtcg cccggactca gtgcggcgct ggcgtccaaa
    37861 ccgtagcgcg ctgcctcggc ctgcatcgct tcttcgagtc ggtcgcccag gcaaaagagc
    37921 atgagcgtcc caatctcatc gagcatcaca ccccgcagac ggtcgctcga cgcgaacatt
    37981 tgcctgacat gttcctcgat cgcgcttcca atcgtgcaga ctccagctgc cacgtgagta
    38041 gcgccgggca gatgatgccc gagcgcgggc gaagaaatcc gagaaccgcc ttcgagtcca
    38101 agccagtccc gcgcgctgtc cacgaccttc cagactcgga aactagcctt cggctgaacc
    38161 cacccctcag catcgatctt cgcgagcaat tcggagagca tagccgtcac acgcgcggaa
    38221 gccagccttt cgcgccgtga accccaggag gcagctatgt cctcgacctc tagtgaaagc
    38281 cgcgggtagg ttatgagcgg catgcggtcc ttcaatacac gcactctttg gcgcactgga
    38341 acatcgccag aacgttttca ggcttcgctt cgttaggaat ccccacaccc gcgtccagga
    38401 tgaagccgct tccgtcgccc acaacgtcga agagtttctt gcagtgctcc cgcacatcat
    38461 ttggcgttcc ggtgatcagc attgaggcag gcacgtttcc tcgcaagacc gccccgcgca
    38521 acgcctcctt ggccttggcc aggtccgtgc gttcgaagaa gtagacgcac ttgccaggcg
    38581 gcacatcctt gatcacgtcc agccggctcg tgcagtcccc ttcccagagc acctgcgggt
    38641 agattcccgc ttcaataagg gccaacagaa ccttctggaa ttgcggccag aagaatgtag
    38701 tgaattgctt cggcgacatg aacccgtcca gcccccagtg cagcggaatg aaaaccatgt
    38761 ttccgccggc cgccttcacg gcctcgattc cggcgcgcgg gatcaacgtc accagcgtga
    38821 ccatcaggat gagcagcctc tccttattgc ggaacaagtc cagcatcgcg cccatggagc
    38881 ctcgcatgta atccgcggct acatcgaagg gcggatgagt cgtgccgtag gccgccatag
    38941 ggaatccaag atccttcagt tcctgtgcga cgcggttgtg gaccgcacgc atcttcggca
    39001 tctcttctcc ggcgcgaatc agcgtttcga gcgcgcggcg cacttcgggg cgggcatacg
    39061 cttccacgga ctgaacgatc cgcgtgtgga gaacgcccgg atacatccgc gactgcgcga
    39121 gaggctccag agcgcccgcg acatgcggca tgtacttgtg aagcatgaaa ccggttgaat
    39181 cgaggatgta ctcctcgtat tcctccggcg ccatgtattg agaatccaag tattggtagg
    39241 cgacattctc ggcgggccct ttgtggcccg gccattcgag ctgcttgtag tcgaggattt
    39301 ccattgtgcg gccgaaagcc acgaggggat gcacgggctg gtaggcatcg ggctcaaggt
    39361 caacgacggc cttgcgaacg gctgcggcga ggcgctcata gtcgcacatc gcctcgcggc
    39421 acgtgattcc gcagtagcgc gctgcccaga aatgagagaa gaaaatcgcg ggcagccggt
    39481 cgggctctcg aagctcgatc gcgtccgtca ggcgcttcag ccgctgctgg taaagagccg
    39541 aggccgacgc agcagcgccg cccgcgctca cgcagggacc cactgcttgc agagggatac
    39601 agcgtccatg gcgttcaggc caaaagcgtc cgcacccgtg taaactttca cggtctcgtc
    39661 cacctgtccg ccgccgatca tgatcttgat gttttcacgc aggccggcgg ctcgaattgc
    39721 ctcgaccgtc ttcttcatcg cgtcgaacgc aagggtcagg aagccgctga ggcccacgac
    39781 ttggggtttg tgttccttga tgctgtcgat gaagttcgaa accggcacgt caatgccaag
    39841 gtcgatcacc ttgaacccgt tgatctcgag catgaacgta acgatgttct tgccgatgtc
    39901 gtgcaaatcg ccttctacag ttccgatgat gaccgtaccc gccgtcttgg ctccggacgc
    39961 gtcctgctta atgagaggct tggtcatatc cccgatcgcc ttcagcattt cgccagcgag
    40021 cataagctca ggcagaaagt attcgccttg ctcgtaacgc ttgcccacga tttccatcgc
    40081 ttcccggcag tggcccaaca gcttcacggc gtcgtacccg ccctccagca tggcgcgtgc
    40141 cagctttacg gcatcttcct cttgcatgtc cgaaatctgt tggaccagtt gcttgctcct
    40201 gtcatccacg atgagcctcc tcaattccct ttcggccgca ccctgccgct agaaatagca
    40261 ggcagccata gggtgcccgt aacgagagcg ggagcatacc tctgttatta agagtgcaat
    40321 atatagcttt cgggggctgt gatatccgtc actaaagatc gtgccacgag tcacgcaaac
    40381 tctcggcctg ggcagccatt attgagtgga ctggagggca agcgtgttga tcagcggccg
    40441 cctggcggcc gcgtgaacgt tcacggaggc atcgggggat ggaaggccaa ccacaaggac
    40501 gggcgcccgc gccgcaattc gaggaacgcg ccaaggcgtg gaagtcagca gaggggatcg
    40561 acttcattag ccccgccgta cgggattcct acgagcgccg cgtacagatg ttcattgacg
    40621 tcatcaccct caagaagccc gaacgcgtgc ctgtttttcc cggaatgggc ttttatccgt
    40681 tcgtgtatgc gggcgtcact gccgaagaag cgatgcacga ctatgggaag ctcgcctatg
    40741 cgcttcgtaa gtttcaaacc gactttctgc cggataccgt gtcgggcact gcgctctacg
    40801 gttcgggtaa gatcctcgaa atcctcgatt acaaactgta tcgctggccg ggccatggag
    40861 ttccgccaaa cgagccctac cagtgcatcg aagccgaata catgcgggcg gacgagtatc
    40921 cacggctaat caacgatccg tccgacttct tcctccgcag ctatcttcct cgagtcctcg
    40981 gctctctggc gccgctggcg tcgattggac ccatcaccga catccttgaa ctgccatcgg
    41041 tggggtcggc cctgattcca ttcgggctcc cgcaggtgca ggaactgctt cggaaactcc
    41101 tcgaggccgg ccgagtcgcg ctcgaatgga ttgaggtctg ccgcgcaatc gacgtcgaag
    41161 cgatgtcaac gctgggcctg gctgggctga tcggcggctt cacaaaggct ccgttcgaca
    41221 tcatcggcga cacgatgcgt ggcacgcgcg cgatcatgct tgacaagttc cgtcagccca
    41281 aggccgtgct ggaggcggtg gaacgatttg tgccgcttgc gatcgacgcc agtgtgcact
    41341 cggcgaagcg ctcgcgcagc ccggtagttt tcataccgct gcataaagga gcggacagtt
    41401 tcatgtcaac cagggatttt cagacgtttt actggccgac gctcaaagcg gtaatcctcg
    41461 ggctcatcaa agagggtctg attccttaca tgtttgtcga aggcgcctac gcgaagcgac
    41521 tcgacaccat cacagatccc gacattccgc ccggccgcac gatttgggtg ttcgaccaga
    41581 ccgatctgcg cgaggtgaag aaacggtttt cgggctgggc ctgtttcggc ggaaacgtcc
    41641 cgtcctccct tctcgtcgcc ggcactgcag acgaggtttc ggagcatgtc aaacggctca
    41701 tcgatgacgt ggggcgcgac ggcggctaca ttctttcgac cggcgcgacc gtcgaccatg
    41761 cgaaagcgga aaacctgcac gccatgatcg acaccggaaa ggaatacgga gttctccgat
    41821 aacgttgaag cgatcgcaac cgttcccgta gcgattgcgc tcccctgcca gcgagggtgt
    41881 actcggtctt gacagttcca cgcatcatct cgacatcctc ggcagtgcat ggctgcccga
    41941 tcgccgccag tgttcgcacg cccagtttgg ctttatcttc tcaacgcctg aaagagtgta
    42001 tgcaagtcct cccgtccttt gttcaagtca ggggctctct gcgacagact gaacacgatc
    42061 cggcgtcgga gaatctctga tttgaacggc tttcagagcg gtgacttacg tcactacggc
    42121 aagtgactaa aatcactgtg gttgcgcgaa cgcctcgaat aggctggggt cgcctcgctt
    42181 caggagccgg tggatgggtg cgattgtcga ggtcaaccgt gaaggcgccc catggctgca
    42241 gttcgccgag ccattcaaca tcgctgaata ctgtgttgac cgccatgtcg ctgaagggcg
    42301 tggcgatcga gttgcgatca agacagccga tcaaactgta tcctacgcac agcttctgga
    42361 gaatgtgaac cgattcggga acgctctcgc gagtctaagc atcggccggg gcgaccgcgt
    42421 tctcatggtc gtcaaggact gccccgagtt cttctatctc ttctgggggg ctgtaaagat
    42481 tggggcgata ccaattcccc tgaatggtct ggcgcccaca ggcgattttg agttcgtcat
    42541 ccggcagtct cggtgcggag cgatcttcca ttccccggat ttcgcccaaa ctattgaggc
    42601 tgccctcgga gcctccacct cgccgccaaa gaccgtgctg cttgtcgaag gtggcgagcg
    42661 atcgatttcg gctctggcac gcaagtcttc ctcggacctc aaagctgttc ccgcaaaggc
    42721 tgaggacgat tgcttttttc tgtattcatc gggaaccacc ggagtgccga agggcgttat
    42781 ccatgcgcac gccgatatcg ccgtcaccag ccagctttat actgttgaaa cggttggcgc
    42841 aaacgaggac gacgcctttt tcagcgtggc gcgcttatgc ttctcctatg gtatgaacgt
    42901 cggcatgacc gggccgctgt ttgtcggggg aacggcaatt ctcgatgagc accggcccac
    42961 gccacagagt gtgatcgact tgtttcgccg gtttcagcca actgtcttcg gggctgtccc
    43021 gacattctat ggacagttcc tggcctccgg cttgttgagc cgggcggacg ttcctcgcct
    43081 ccgaagatgc atctcagcgg ccgaagcctt accacccgaa ctacaccgcc aatggctgga
    43141 agttaccgga gtgccgatca tggagggcat cggttcgacg gaagtgggcc atatttacat
    43201 ctcgaatcac gtagacgaca ttcggccggg gattacgggc aagccagtgc ggggctacca
    43261 agtcaggctg atcgacgagg acgggaacga cgtcgccgaa gatgtgcccg gtcgcctgct
    43321 ggtgaagggc caatccgtga tgaaacgcta ctggaacgat cccgagagga cggcgaaggc
    43381 gtttatggag ggctggtaca acacgggcga cacattccgc cgcgacgcac aaggttatta
    43441 catctactgt gggcgcagcg atgacgtatt taaggtcagc ggccgatggg tttctccttt
    43501 cgaagtcgaa tcggtcctga ttcagaatcc tcgcgtcctc gaagccgcca tcatcggcag
    43561 gagggatgag cacggcctga ttcgcgcgga cgcctgggtc gtcttgaaag acccttcggc
    43621 tggcagcacg caggcggaag aggagctgcg ccatttttgc gaagccaatc tcccacgcta
    43681 caagctgcca gcgagaattc acatcgtcga gaagctcccg aaaacggcaa ccggaaagat
    43741 tcagcgatat aaactgcgcg tcacttctga acacgaagat cggacgcccc cccaaaagag
    43801 agaggatgcc ttggtctcta tcgagcgcgc cgggcgggtc gtcacggtaa ccctctctcg
    43861 ccatccagtc aacgcgctca atgacgagtt catcgatcgc cttgaagctg tcctcgatca
    43921 ggtcatcaag gatgaagaag taatggtgtt gcatatccgc agcgaccaga aagtcttctg
    43981 tgccggagcg gatctcgcgt tgatgcagtc gtgcttcggc acgctcgaag ggccggacgt
    44041 catgctggat ttggtcgttc gcatgcagcg tctgtacgca aagatcgaag cggctcccct
    44101 gattacgctt gccgaaatca acggcgcggc gatggggggt ggactggagt tcgccttggc
    44161 atgcgatctc cgcgtcgcgg cgaacgaagc gaaactcggc ttgccggaag taagtctcgg
    44221 ccttctgccc ggcgccggtg gtacccaacg tcttacgcgc ctgtgtggtc acggtgtcgc
    44281 gagccgcctt atcctcagcg gcgaggtaat tgacggcgtc atggcggagc ggcttggcgt
    44341 cgttcagtgg tctcaaccgc aatcccagct aggcgagtgg acgcgagaac tctccgcccg
    44401 gctcgcgaag gctcccaggc cggcgctcgc tgccagcaag cgctgtcttg cagccgcggg
    44461 cgacccgact cgcgacggct ttgccgagga actcgctgct acgcggaagc tctatcaaca
    44521 tccggagaca cggcacaggg tttcggagtt tcttgagaga agcgcaaaga ggcaccagac
    44581 aacgaaggcc tagaagagcc gatccgtgca gatttctttg gaggaaccat gaagctcaac
    44641 ggcaaaagtg caatcgtcac gggggctgct tccggcattg gcctggccac ggcggatact
    44701 ttggcagagg cgggcgccct cgttgtcatc gccgacatcg ctcgagacaa gggtgaagcc
    44761 gccgcggtcg ggatccagaa gaagggccac gaagccgagt acgtccacct cgatatcacg
    44821 gacgataaat ccatcgcggc gtttgtcgaa accgtccaga gcaaagttgg cgctattgac
    44881 atcattgtga acggcgcggg ctggggccag ataaaaccgt tctgggaaat gccgctcgat
    44941 gtttggagca agatcgtgga actgaatctg gtcggtccga tgaagcttat caaagctctt
    45001 ttgccgaaaa tgatggagcg caatggcgga aaaatcgtaa acgtcggcag cgacgccggg
    45061 cgagtcggca gctcaggcga gacagtctat gccggcgcta aaggtggcct gattgccttc
    45121 acgaagtcgc tggcccgcga agtggcgcgc tacaacatca acgtcaattg cgtttgcccg
    45181 gggcctacgg agacgccgct cttgtctgcg gtacctgaaa agcacctgga ggcgttcaag
    45241 cgggcgatcc cgtttcgccg attcgggaag ccgcaagaag tcgcggacgc gattctgttt
    45301 ttcgcgagcc ctcgctccga ctacatcacg ggtcaagtgc tgagcgtcag cggcggtctg
    45361 acgatggttg gctaatctag atacagggag cagaaacatg aaacagatac aattggcaaa
    45421 ctacaaggcc agccatttcg gttggagcgt ctcgggcaag accgccacca ttacgcttaa
    45481 tcgccccgag cgaaagaacc cgattacgtt tgaatcctac ggagaattgc gggacctttt
    45541 ccgcgaactc gtgtatgcca ccgacataaa ggccgtggtg ttcacaggcg caggcggcaa
    45601 tttctgttcc ggtggcgacg ttcacgacat cattgggaag ctcgtgaaaa tggacatgcc
    45661 cgaactgctc gcgttcacgc gcatgaccgg cgatcttgtc aaagcaatgc gctcgtgccc
    45721 gcagccgatc gtttccgcca tcgacggcat ttgcgcgggc gctggggcga tgatcgcgtg
    45781 tggttccgat cttcgatttg gcacggcgcg cagcaaagtc gcgttccttt tcgtgcgcgt
    45841 cggacttgcg gcagccgaca tgggtgcatg caccttgctg cctcgtctga ttggcatgtc
    45901 gcgggctgcg gacctgctct acacgggccg cgccatgagc ggagaagaag ccgagcgttt
    45961 cggcttttac aaccgccttg tggaaccgga gaaactgctc gaggaggcaa cagccacggc
    46021 cagttctctg gcggatggcc ccacattcgg ccacggcatg accaaaacca tgctgtacca
    46081 ggagtggagc caaggcctcg atgagtgcat cgaggcggaa gcgcaagccc aagccatttg
    46141 catgcagacg aaggacttcg agcgcgccta cgttgcgttc gcgtccaaga agacacccgt
    46201 attcgaaggc aactgatggc agacaagagc tttctcacgt ggcccttctt tgaccacggc
    46261 catcgaattc tggccgacga actggagctg tgggcgggag aatctctccc accgctgctc
    46321 aagggctcag aggacaattt ggattccgtc tatgcctgtg ttcgcaggat cgctgctgag
    46381 ttggggaaag cagggttgct gcgcgtttgc gtccctctag cctatggagg ggcgcgcgac
    46441 gatctcgatg tgcgcagctt gtgcctgggg cgcgagatcc tgggcagggc ttcagggctg
    46501 gcagactttg cgcttgcgat gcagggattg ggcagcgcgc caatcacact ttttggccgc
    46561 gagaagccaa agcgcgagat tctgccgcgc gtgtccgctg gcacagcact cgcggccttt
    46621 gcgctttcgg aacccgatgc cggttccgat gtcagcgcca tctccacaac cgcgaaacgc
    46681 gacggccgat tctggcgcct tgatggcgtc aagacgtgga tttcgaatgc ggggcttgct
    46741 gcgttctatg tcgtctttgc tcggacgggc gaagggcccg gcaccaaagg tttgtccgcg
    46801 ttcatcgtgg atgccgaatc tgcgggtctc gatgcctccg aacacatcga cgttatcgcg
    46861 ccccatcctc ttggcactct gcgtctgagc ggttgtcagg ttaccgatga ctcgctgctg
    46921 ggtcagccgg gcgaaggctt caagattgcg atggccacgc tcgacgtctt ccgcacgaca
    46981 gtaggcgcgg cagctttggg cttcgctcgc cgcgctctgg atgaggcagc aggccgggcg
    47041 ctctcgcggc gcatgttcgg ccatttgcaa tcggaattcc agctcacgca agaaaagcta
    47101 gcagacatgg ccgtgtccat tgacgccgca gccctgctca tatatcggtc agcatggacg
    47161 aaggacgtct tgggagggcg cgtcacgcgc gaagcttcga tggccaagtt gtttgctacc
    47221 gaatcggctc agcaagttat cgacacagcc gtacaaatct tcggcggcct tggagttgtt
    47281 cgaggggtga ccgtggagcg gctctaccgc gaaatccgcg cgctgcgcat ctacgaaggg
    47341 gcgagcgagg tcctcaagct gatcatcggt tcaaaggtgc ttgaggcagc cgcgggaggc
    47401 cgtgggggcg cctcttcctg agacgttcac gaggtatttt catggacgtg gatatcaaac
    47461 gaccgctggc gcgccaccac gcggttatca cgggcggagg gcgcggaatt ggggctgcga
    47521 ttgcggaagc cttggcgcgt tcgggagcgt ccatttcctt ggtcggccgt aactccgaag
    47581 tgctactggc aactgctgag cgcatcaaga atagctatgg cgtgaaagtg gcgacagcct
    47641 cggcggacgt ggccgacgaa gccgccgtgc gaagggctct tgcggccctg ggaaatgcac
    47701 tcggcgctcc aacgattttg atcaacaatg caggtgtcgc cgtttcagcc ccgttcctca
    47761 aggcagatgc ggagttctgg cgcaaggttc ttgacacgga cctcatgggc gcagtttact
    47821 gcacgcaggc tgtccttcct gcgatgttgg aagctaagtg ggggcgcatc atcaatatcg
    47881 cgtccaccgc aggtctcgcc ggatccgcat atatcactgc gtactgcgcg gcgaagcacg
    47941 gtttgatcgg cctcactcgc gcccttgcag tcgaaacagc gcgtacgggg gtaaccgtca
    48001 acgccgtgtg cccagggtat gtcgacacgg aaatgaccgc ccagaacgta gcgaacatca
    48061 tgaagaagac tggccgaagc cgagacgaag tagttgccag cctcgtgggg ggcaacccgc
    48121 aggggcgtct cattcagccg gcggaagtgg cggatgccgt catctggttg tgtggcgaca
    48181 acgctttgtc tgtcacggga cagagcattg ttatcgcggg cggggaaatc caacaatagg
    48241 atgcattcgc aagtctccgt gacttccgtc gcggaatcct ctacgaaggc ctcggctagg
    48301 aataagcggc tatccctgta atgcgctcac cgatgatcaa tcgctgaatg tcgtgcgtgc
    48361 cctcgtacgt gtagacggac tcaaggttgt tcatgtcgcg catgacgcag taatcctcca
    48421 ctgtgccgtt ggcgcccaga atgtcgcgcg ccatccgagc cgtctccctt gccatccaca
    48481 cattgttcat cttgagcatg gatacatggg aaggatgcat ccgagcccaa tcctccagcc
    48541 ttcccacatg cagcgcaagc agttgcgcta tggagatctc cgtaatcatc catgcaagct
    48601 tgtcctgtac gagctggtgc gacgcgatcg gtttgttcgc aaactgcttg cgtgacttcg
    48661 cgtatcgcag cgccgtgtcg taacacgcca ttgcagcgcc aattgcaccc catccaatcc
    48721 cgtatcgcgc ctgcgtaaga caagaaagcg gaccccgcag gccgtccacg cctggcaaca
    48781 tgttttcggc aggcacttcg caatcgctga atgaaagtcc cgaagtaacg gaggcgcgca
    48841 tcgacagctt gccgtggaca tcgtgcgcct taaatccctt catgcccttt tccaccagga
    48901 agccgcgcac acggtcgtct tcgcacttgg cccacactac gcacacgtcc gcaatggaac
    48961 cgctggtgat ccacatcttt tcgccgttga ggacgtaccg atctccgcgg cgcacggcac
    49021 gggtaagcat ccctccgggg ttcgggccga attgcggctc tgtcaaaccg aaacagccga
    49081 tcgcctggcc actttgcaag cgcggcagcc atttgtcttt ttgcgggggc gaaccgtacg
    49141 aatgaatcgg gtacatcacg agggcgctct gcacggaaac aaaactgcgg agaccagagt
    49201 cgccgcgctc aagctcttgc atcacaaggc cgtaggccac gttggacatc tccgcacaac
    49261 cgtagccgtt cagattcgaa ccgaagaatc caagctcccc caactgcggc accaattcca
    49321 ccgggaaagt cgcgtcccgg tagtgcttct caacgatggg aagcaattac tcatcgacga
    49381 actggcgcgc tgtctgggcg cgcgaggagc tcgtcgtcgc tgaagatgga atcacaatcc
    49441 aagtaatcca cgccggaaaa cgcgggcatt acgcctcccg taattgcaga actgagtgcc
    49501 tgatcaacct cgccgaagca gggtaccgcg cgaactcgac aaccagcctt atcgtgctga
    49561 aagccgagtg ctgtgatgaa ggtcactgat gttgggcatg aacgcgtcca gacttaggcg
    49621 cggttcgatt gcaaaacaat cagcggaacg acattgatga cttcgggcgc gctacttcaa
    49681 agcagtctcg tcgcagtgat cgggagcgga accatgggag caggcatcgc acaagtggct
    49741 gccgcttcgg gccatcccgt gctcctgtat gacacgcgaa ccgaggccgt ttcgacggcg
    49801 attcaaagcg tacgccagac ctttcaaaag ctttgctcca aagggaatca ttccgcggag
    49861 tcggcagaga aagcatcagc gcgattgcgc gccgcgacga acctgcagga agttagcggg
    49921 gcgggaatcg tcatcgaatt cgtcgtggaa gatctagagg tcaagcaaca gctgtttagg
    49981 gaagtcgaga agatcgtgga tgaagattgt ctgctggcaa cgaacacgtc ctcactttct
    50041 gttagcgcca tcgcagccgc gctcagggct cctcagcgac tggtgggcat gcacttcttc
    50101 aatccggcgc cgctgatgga actcgtcgaa gtgattggcg gcgtagcgac cgatcagcag
    50161 ttccttgcgg ccgtctatgc aacagctgca gcgtggggca agacaccggt ctacgcgaag
    50221 tcaactccgg gggtcattgt gaaccgtgtc gcacgacctt tctatggaga ggctctgctc
    50281 ctgctcaccg agcaagcggc tagcccggcc acaatagatg ctgttatgcg ggagtccggc
    50341 gggtttcgaa tgggtccctt cgaactgatg gaccttgtgg gcctggatgt cggctttgct
    50401 gtgagccagt ctatattcaa cgcttacttt ggcgaccctc gataccggcc gtctccgatt
    50461 caacaggaaa tggttcgcgc gggatatctg gggagaaaag ccggccgcgg gttttatgag
    50521 catggcgaag acgcgcgcct tgcgcagcca agtacggaac cgcctgctcc taaacctaat
    50581 cgcgttgtag tcccatcgga gggtcgtctg gctggggcat tgacgaaacg gttggcaggc
    50641 acaggcgcag aaatcgtcag cggccgcccg cgaaatgact gcgcggattg ggctgaagta
    50701 ggcgacacaa tgattgctgt gacagacggg cgtagcgcga ctcagcgagc gcgtgaaacc
    50761 gaacatcgaa accttgtgct tgccgacttg gcgctggact acacaaaagc cacgcgcatt
    50821 gcagtcacgg cagcgagcac atgcgatgaa catgcatatc tttcggctgc aggactgttg
    50881 caggccgccg gattcgcagt ctcgcatttg aaggatgttc ccgggatagg tgtgatgcgg
    50941 acggttgcca tgctggccaa tgaagcagct gatgccgtga atcaaggcgt ggccacggcc
    51001 gccgatgtgg atatggccat gcggaagggc gtgaactatc cgctgggtcc gttggaatgg
    51061 gctgatgaat tgggactcga aattgttcaa accgtgctca tgcatatcgg cgcccactac
    51121 ggtggggagc gctatcgcat ctctcctctg atccggcagc ttgtatggag cggcaacaag
    51181 tttcatggcc cgcatcttca gtgacgggag ccatccatga acgaggcgat tatttgtgac
    51241 gcaatccgca ccccctttgg ccgttatggc ggaagtttat cttctatccg cacagatgat
    51301 ctcgctgcca tcccgattcg gacattaatc gaccgcaatc cgaaagtgga ttggcaggca
    51361 gtagacgatg tgatcttcgg ctgtgccaat caggccggtg aggacaatcg caatgtcgcg
    51421 cgcatggcat ctttgctggc cggtctgccg caggatgtac cggggacgac agtcaaccgt
    51481 ttgtgcggat ccagcatgga tgcgattgga atcgcagccc gcgcgatcaa gtgcggcgag
    51541 gcggacctga taatcgcggg cggcgtggag agcatgtccc gggctccctt cgccatgggc
    51601 aaggcggaga cgccattcag tcgcagtgca gagattttcg atacgacgat cggatggcgt
    51661 ttcatcaatc caacgatgaa gaacaagtac gcaaccgatt ccatggccga aaccgctgag
    51721 aatgtggcga atgattttca catcgcgcgt ttcgatcaag atgcctttgc cttacggacg
    51781 cagcagaaat gggcgaaggc tcaggcggaa ggtttctatc ggtccgagat tctacccatt
    51841 ccaattgccc aaaagaaagg cgagccgaag acattcgacg tggacgagca tccacgcccg
    51901 gatacaacac ccgacaatct cgcgaagcta aagcccatcg tgaaggcgga tggaacgatc
    51961 acagcaggta acgcgtccgg aatcaacgac ggttcctgtg ccctgcttct cgcctcggag
    52021 gccgcggttc gcaaattcaa gctcactccc cgcgttcgag ttctggccac atccgtcgcc
    52081 ggagttgcgc cccgtatcat gggcatgggg cccgtgcccg ccactcgcaa gatccttgcg
    52141 aagacaggac tatcgctctc gcagatggat gtgattgagc taaacgaggc atttgctgcg
    52201 caagcactgg ccgtgctacg cgccctcggc ctcccggacg acgacgagaa cgtaaatccc
    52261 aacggcgggg ccattgcgat cggccatccg ctgggagcga gcggagcccg gctggtcacg
    52321 acggcaatgt accagttgca cagagggaaa ggccgatacg cactttgcac catgtgtatt
    52381 ggagtgggac aggggattgc tacggtcctg gagcgctgct gaaagaagga taatgggcga
    52441 agggtcgttt gcgccgggag acgccagact cgcgtgagcg gataattgtt cttgactcac
    52501 aaagaggtat tatggtactt gaatgcgtat taaggagcca tcgatgagta gcccaacgcg
    52561 cattaccgat aattcaggaa atacagggag aacggtgagc gacctcgtcc tggagaagcc
    52621 cgcttctgcg cgagtattcg aatcgcttgg gatcgactac tgttgcggcg gcagtcaaac
    52681 gcttgcggag gcgtgcaacg cagccaacct ctcaatcgag gaagtcacgg ctgccctcga
    52741 gaagtgcgag cccacctccg cggagaaaga ctggcgcggc gagccgctgg ccgatctcgc
    52801 gcgccatatt gtggagaagc atcacacgtt cacgcaggct gagatcagtc ggctgagcgc
    52861 cttgattgca caggtagtcg ctgcgcacgg caagaaccat cccgagctgc cgcgattaca
    52921 aacggtcttt gcagggctct cggaggaact gcgcgagcac atgagaaagg aagaggagct
    52981 gctttttccc tatgtcgtcg agatggagga ggcggcccga actcgacgcc gcctgccgga
    53041 ctccatgttt ggaacggtgc agaatccggt agcggcgatg atcatggagc atgaagcctc
    53101 cgggcaggcg ctcgaaaaga tgcaggaaat cgcccataac tatgcgctgc cgcccgatag
    53161 ctgcgccagt tatcgagcgc tatatcaagc gttgcctgca tttgccgcag atctgcatca
    53221 gcatattcat cttgaaaaca acatactgtt ccctcgcgcg gttgaacttg agaatagcct
    53281 gtccggatct gcgcgatagg gacactgtgc agaagacttg caaagaaaca gttgaggaga
    53341 aatgaaatga aaacggcagc cgcgaaagga aaaaccgcaa aaccggaaat cgccggaccg
    53401 aatgtggacg cagtcctgcg catcatggac gcgatggaaa aggcatggag cgacaaagat
    53461 ctgccacagc ttttcgatca ctactggcat aacgacggct ttgtcctgtt tacatcccgc
    53521 ggcgcttatt acggctggga agcagcgaag cagatgctag agcaatattt cgataatctg
    53581 gaagaagtca gcttgaaatt cggcccacgc aaggcaaggg tgtttggcga cgtcgcaaca
    53641 atcgtctacg aatggcgtac tgatggccgg tcgacattga atggctcacg tcttctacga
    53701 gaaggttacg gaacagacgt ctttctgcgc gaccatgaaa cgtggaagct ctatcacaat
    53761 catgtttccc tcggacgcgc tggaactagc catatgcagg atcatgcttg gtagggacta
    53821 tgaatcacat ttctgttgtg agagctcgat tagatagagg aacagcggat gagatacgca
    53881 ctcggccagc cgtcagttcc gatctggcgg cattgatcgc cttagatgag gaagtcaccg
    53941 gcattgccaa acgaaagtat tggaaagagg cgtttgcgcg ctacggggcc agacgccgtt
    54001 tgccgcgatt cttcctgatt gccgaacaga atggtcgtat agaggggttt acgatcggcg
    54061 aggtgcgcgc ctgggaattc ggcgaacctc cttgcggttg ggtctttgcg attcaggtgc
    54121 gtccgggtag ccgtttgaat ggagtcggca cacgactctt cgaggctctt tgcaatgaat
    54181 ttcgaaaggc cggagtcaag agagttcgga caatgatggc ccgggataat cacctggttc
    54241 tttctttctt ccgcagccag gggatgatgg cgggaccgtt catcgagctc gagagcgcac
    54301 ttgattagcg catataaagg aagcggggca tgaaactaca gaaggcaagc gcatttgcca
    54361 tctacgctgt gctcgagctc gccgcaaatc cgctgcggca attggctggg attgatatcg
    54421 cgcgcgagta tggcatttct gcgcatcatc ttgcgaaggt gctgcgtgac ctcggacggg
    54481 ctggaatcgt cgaagcggtt cgcggtgtcg gtggaggcta ccggctctcg gtgaatccga
    54541 agcggcttac gctgctcgat gttatccaag ttttcgagcc cgttggcccg caaaacgata
    54601 caaccgaaga cccggaactt gcgactgatg caggacaagc tctgcgcatg gtgctcgatg
    54661 aactggatga ccacgcgctt gcaacgtttc agtcgatcac aatctcgacc atgctgaaac
    54721 tgatggcgcg ccgcccgtgg gcgggctcag cgcgcgtatc gaagcagggg cccagagcgg
    54781 catcccgtca ctgacgccgc cggtgcgttt agggaggagg ggaatatggc ttcaacttcg
    54841 gtccaggaga tttcgacgct cgattcgact ctggcttcgt gtcgggagat tgcccaggat
    54901 cctgagttct caactgccaa gcgctggctc gaggagcacc ccggcaagaa ggccatcggc
    54961 tgtttcccga tctactgtcc catagagctc atccatgcgt ccggaatgct gccgctgggc
    55021 gtcatcggag caggcaatca aatcgaaatt acgcatgccg attcccggtt tcagtcgttc
    55081 gtctgctcga ttgtgaaaag cacgctcgaa ttgggactga cgcaaagact caagtttctc
    55141 gacgggatgc tttttcattc catctgcgat ccagcaagga atcttgcgag cgtcttccag
    55201 aggaacttcc ccgaaatgtt tgtggagtat attcactttc cacagaattt cgcatcagtg
    55261 ctggcaaggc agtatctgga aacggaatac cggcgaattc tccaaggttt gcagcaaaga
    55321 tcgggacaca cgccgactac tgaagatttc aatcatagca tcggcctgta caaccgcata
    55381 cgcgccgcct tgcggaagat ctatcagatc aggaccgaca caccgcattt gatctcagcg
    55441 gccgagtcgt tcgtgctcat gcgtgccggg acgttaatgg ctccggaaga ctttctgaag
    55501 attctcgaac aggttcttgc ggaaatcccc caacgcccct ctcggccaaa ggatcgaatt
    55561 cgagtcgttt tggaagggtc attctgtgaa ctacctccag ttggcctcat cgagacctta
    55621 gaacaggccg gttgctacat cgttgacgat gatttcctgc ggggctggag atggttcact
    55681 gacgatgttc ccattcagga cagccccctg gctgcgcttg cggagagcta ttcagagcgc
    55741 gcgtattact ccggtaccaa acatgacacg cgagaaacga aggcagccca cctgattcgc
    55801 ttggccaaag agaagaaagc agatgcggtc gtattccagg ctgcgaagtt ttgtgagccg
    55861 gctctattcg actatgccct ttaccgcaag gcgctcgaag acgcgcagat tccgcacctg
    55921 ctgttggaat tcgaagagaa aacgtggagt ttcgatcgcg cgcgctcgga agcggaaaca
    55981 ttcgtggaat cgctgttgtt tgattgacga agattggttg gcgaaggagt ttgatcatgg
    56041 cacaagcacc agccgcagca aagaccgact atcaagggcg catccataat cttcagaagg
    56101 acatgatcgg tcgctattac tcgcgattga cggcggcagc ggaaaacaag agcgagaaag
    56161 cgatctacat gcttatcagt ggcaatcccg tggagttgac cgaagccttc ggtctgattc
    56221 ccgtgtaccc agaggtgaat gccctacaac tcgccgtgaa gaagcagtcg ctgccgctca
    56281 tcctggccgc ggaagaaatg ggctactcga cggataactg cgcttatgtg aaagcggatg
    56341 taggcatgta cttcgccggg cgcaagacgc ctttcgccac tatcccggaa cccgatctca
    56401 ttctttgcaa ctatgtcggc tgcaatgtgt acctgcaatg gttcgagcat ttggctgagt
    56461 attcgaaagc tccaatctac aacttggacg tcccgttctt tcgcaccacc agtgacgatc
    56521 cctccgagga agacgtgcgt tacgtagtga agcaactgga ggagctcgtc gaaatcctgg
    56581 agcgcatgac cggacagaaa ctcgactaca agaagctgca gcgaatcgtc gagctatcgg
    56641 gcgagacggg agcgctctgg tcggaaatca agaggctcac catggagcgt ccggctccct
    56701 atgatgccta cttcgattcc gtcatgatga tggccccgct gtactgcttg cgtggcaccg
    56761 aagatggatt gtggttcttc caggaaacct acaaggagat gaagaagcga gcggagagtc
    56821 acgaagggcc gctcccggaa gagaagttcc gcttggtcat ggaaggacct ccgccgtggc
    56881 catatctgcg ccagttccga gacatgttca gccgctgggg cgctgtggcc gtggcctcaa
    56941 catactcgac ggttggaggc atctgggaat tcgggttccg gcacgacccg ctgcggccgc
    57001 ttgagagcat cgcccgacac atgctccagt ggaatatgac gaaccgcaac ttcaggcagc
    57061 gataccagca gatcaagaag tacgtggaag actggaaggc tgacgccctg gtcatccatt
    57121 ccgtaaagag ttgcaggctc ttctcagcgg gccagggcga catgcgagag tacttcagca
    57181 agcagctcgg cgtgcccact ctcctgattg aatcggatct ggaagacccg cggtatttct
    57241 ccgaagccca gttgcggaac cgcgtggacg cgttctttga ggcactagag caccagaaga
    57301 tgctagccgc gaagtagcag gcagttatcg aggcattttg ttgtgagaag tgcaaagaaa
    57361 ggcggggctg tatgacttac ggaggcggag tagacgtagg atcgacgcaa acgaaagcga
    57421 tcatcctttc ggacgaccgc aggattgtgg ggcgcgctct gatcaataca ggcgcaaacg
    57481 tttcacaggc ggccgagcgc tcatttcgag aggcggcggc ctccgcggga attgctcccg
    57541 aaaaagtcgc ctacgtcgtg gggacgggct acgggcgcta caaggtgaat ttcggaaacg
    57601 cccagatcac cgaaatctct tgtcacgctc gcggcgcaaa tttcttgttt ccaaacacgc
    57661 gtaccgtcat cgacatgggt ggtcaggatg ctaagggaat tcgcgtcggg gaaaacggcg
    57721 acgtcaaaga cttcgtgatg aacgacaagt gcgctgccgg gaccggccga tttctttcga
    57781 atgctgcaga aactgtcgga ttaagcttgg acaagatcgg cccgatttcg cttgaggcta
    57841 agaagcccgt acggctcagc acggtctgcg ccgtctttgt ggagtcggac atcatgtcct
    57901 atctggcgga gggcaagact gtgcccgaca ttctaggcgg cgtgcacagc gctatcgccg
    57961 ctcggaccgt tgcactggtg cgtcgcgtag gaatcgagcc ggaaatcgcc tttacgggcg
    58021 gcgtttcacg caacatcggt atggtgaagg cgcttgaaga gaagttgggc ctgccggtta
    58081 acgtctgcga cgattcacat ttcatcggcg cgattggcgc ggcgatcttc gcgctggagc
    58141 gtgcgctcgg cggagagact ggcgccggtt ccgctaaggc gaacccagtt gcggcatcgc
    58201 aggcgtcaca gccgagtgct ggagcgcact aaggacgggc acgacttaga agaggacaac
    58261 tgaggtgctt tatggcatac gtagttggaa tcgacatcgg ctctagaagc acaaaggccg
    58321 tgatattgga tgaatcgcgg cacgtctgcg gcaccggact cgtgaaaacg cgcccggact
    58381 tccctggggt cgccaaggaa gcgatggaac aagctctcgc caaagctggc gcccagaagg
    58441 aagacgtgaa gtatgtcgct acgacaggcc tgggccgata caacattccg ttccgggatc
    58501 ttcaaattac cgatatcacc tgcgcggcaa ggggtgcaga attccttttc ccgaatacct
    58561 cttgcgtcct cgatgtcggc gggcaaagca cacgggccat ccggctgcgc gcgggcggca
    58621 aggtaaagga gttcaagtcc aacgacaagt gcgccgcagg ttctggcgga ttcttggaaa
    58681 gggcagcgca ctaccttgaa atcccgctta cgcaactcgg ccccctttct acgaaagctg
    58741 agaaacccac gacgatcagc agcgtttgcg ctgtgctggc agaatcggag atcatcaacc
    58801 acgtcagcga ggggcagagc gtcgagaaca tcatccgcgg catccacaac tcgcttgctt
    58861 cgcgttccaa ggcgttgctt tcccgcgtcg gactggaaga cgaagtcact ttcgtcggtg
    58921 gcgtggcgcg ccaaagtgga atggtggagg cattgaagca gacgatcggt cgggatgtaa
    58981 atgtcgctga ggaacccgat acagtcgctg cactcggcgc cgctctgctg gcacatcaac
    59041 gtctgcaaaa acttggtggg atcagcaagg aaaagggcac tgacccgtat gtctatggtt
    59101 agtgagaagg aacgctgcgg gacggcaagt gtagtcgatc ttgtcttgca gtgaatctcc
    59161 ttgggaggcg cttctacgtc gcattagaag cggcaacatg tccaagtcct tgtatcgcgc
    59221 caaacggttt ggaattcatc ctgggcgtgc ccccgcacaa tgtgccatga gccgcctcac
    59281 catgttaggt gacatatgtc acaacccacg ccacaataat ctgctatcaa tcgtcgcgct
    59341 tctaacggca gcagcatgag gtgaataccg caacatatga ctcgggtggc tccaagtcga
    59401 agaacggtaa acgctagcat gaattcccca gcccgcgttc aatcgatgaa tacagctccc
    59461 gcgggcttcg atcccgccac agacctgcca aagggtttcg ttgacttcct ccttcctctg
    59521 gttcaaccgt tcacacgccg ccagcgggaa ttggttcaga agagagctgc ggcgctaacg
    59581 gcggctcacg aagggaaact gccggactat ctcccgccct cgccggcgac aaaggacgac
    59641 tggcagatcg agctccccga ctggtgccgc gaccagcgaa atcagatgac cggccccgcg
    59701 gacgacgctg aactcgtggt taagatgctg aattccggcg cgcctggcgt gatgctcgac
    59761 ctcgaagatt ccatggccaa cacgtggcca catctgatgc agggcgtcgc caatgtactg
    59821 gctgcgctgg gcggcactct cagctatttc gatcgcaagc gaaaccgcac cgtctccatt
    59881 cagccaagta agtccgcgat attcgttcgt ccgcgcgggt tgcacatcca ccaggcggga
    59941 gtgttgcctg gagaaatctt gccggcgtcc ctcttcgacg tggcgctgat cgcattccaa
    60001 gtgaaccctg gagagatgaa gcatccgtta acgttctaca tcccgaagac ggagtccgcg
    60061 gaggaggggc tctggtggcg cgacctattc caggtggtgg ccaaggccag gggatggcgg
    60121 aaggacgaaa ttaggtgcat ggcgcttgtc gaatcccatc cgcttgcata tcagatggag
    60181 gaattcgcct acaaccttcg cgagcacatg ttggctctga accttggcag gtgggactac
    60241 atggcgagcc tcatccactt taatcttcgt gatccgcgct gggttctgcc agatcgcaat
    60301 acgatcccgc acgacgtatc cttcttccag aacatccgca atcgcatgcc cgagatttgt
    60361 cacaagcgcg ggatgcttgc cattggaggg atgaccgcgc tgtatcccag ccgcgaagac
    60421 gcggaactga atgcgcgcgc tctcgccatg cttgagaagg acaagaagaa cgaggcgttg
    60481 tgtctgatgg acggcgcgtg gaccggccat cccgaccaga acgagatcgc agtgaagcag
    60541 ttccctttcc cgaaccagtt gcacgcccgg ccttccgatc ctgagccccg gccagacctg
    60601 cgccctattc cggaaggagt aggcaagcga acgcttgccg gtacccgcgc ggctgttcgc
    60661 actgtgatcc gttaccgcca gggcgtgctc gccggccgtg gcgcgagtct tcttgacggc
    60721 tatatggagg atctggcaac cgaccgcatc taccgcctga tgatcgccca gaggatccgc
    60781 catcgcgatt ccgttcccat tgtggacgat ggcggccagc ccgtccccca cacacctcag
    60841 agaattacgc aactgttcga cgaagaactc tcccgcattc tgcgcgaatt gccgggagac
    60901 gcgtctccgg agatcgggga gcaatttagg caggcccgcg cgctcagcga gaagttgatt
    60961 gtgtccgggg agttcacgcc gccatgaagc gggaggttgc tgatactaat cctggtttat
    61021 cggataagcg tgcgtgtgct gtgatgtcgc tcgtttcagg tattctcgct cgaatcggaa
    61081 aagacgcctt ccatcagcca aagtagtaac gaacaatgca ttccgccacg caacatggtt
    61141 tctctgcccc gcggcattcc agcacaactg aataggttgc ttctatggcg tctggaagct
    61201 gttttgttga ctgaacgctg acgcgagcat gaatctgaga ctcggcgcgg acgggcgatg
    61261 ggaaccgcac tcgattaagg ccataattca cccgcatccg gaccccgcct tggacctgga
    61321 tcgcctctct caagaagcgg ctgagaagcg aaagagtcaa gaatccgtgg gcaatagtcg
    61381 tgccataagg tgattctctc tgtgctcggt ccgggtctac ttgaatccac tgtcgatcgt
    61441 ccgtgccctc agcaaactgg cgaatcattt cttgcgttac cgcgaaccat tccgttacag
    61501 cgatctcccg gccgacaaag tcgctcaagg cctgcggtgc ttcaatgaca agcggagaca
    61561 taagatcgtc ttagtgcggg cgattcacct ttctgcacat agggcggcaa ccacgaagca
    61621 ttcccgtctt tagggagtca gacggccgct ggctcaggag atgacctggc ccaggcccat
    61681 ctgtcctact ttttgcgctc tcgcgccttc cctccacaaa ggactgctcc ttcgctgccg
    61741 aacagaagca cttctacacg tgagcaagcg tcaggcaaat cgaattattc cttcctttga
    61801 ccgtgcggct taagcattac gagtttctta cgctcgacag gtgacgacgg tcccaatatg
    61861 gcgagcgcaa tagattcgaa cctcgagccc gtttttgcaa accgcgagat tcgcttctaa
    61921 cagctgcgtc gctcaatttg acaactagtg attaggacga atctcggaat ctgctttcgc
    61981 ccagaagcga acgccaatag accgaaaaca ggggcttcta tcgcgcctcg attttcttgc
    62041 ggagttggac tatttgtgac taatttcccg aaaaaaaaac gggtgaaaat ttgccaataa
    62101 cactgatttt tcgaaaatca gaaatcggcc gaaaactcat tacagcacac gatttttcca
    62161 ataatgttgc gaaaaatctt gcgagcatcg agggactccc ggtatcgcta ccaacccttc
    62221 ctaagtgatt catttgacca cggttgatag cgtgtctagg gcttgtccca aatttgtccc
    62281 aaacaatcga ggctttgccc tgaaagtgag aattgcgggg catggcaaac acgactgtaa
    62341 gcatccgagc aaaagtcaag atcgaggggc gctggcgttg gagttgcagt ctccgggccc
    62401 cggaaggcaa tctcacgcca tctgaggcgg agcgacaggg caagttctac ttggtgtgga
    62461 cggagggagg gcaagaagcg cgagcagagg gtcaagggga atttcgaggc tgcggcaaag
    62521 gttgcaaggg cgaaagcgcg gcacctcgaa gatgccgctg atgccttcga acgcaaagcg
    62581 agaactatac gcctcatcca gtttgaaacc tgggcgttcg ttgagtgctc gggacgtcca
    62641 attggcggta aatcatgact tccgagatgc ggacagcact atcgtggccc gaccgctacc
    62701 cctcgccgcc gatgtcgcct tctccgagta cgcacgcatg ccactgatca tccgctgcgt
    62761 gcgttgcgca ataccactga ccgcagtttt cgcagcgcat ggtcgctgga gcgtcgcagg
    62821 gctcatccaa atgagattgt gcctggcaca ccggtgccga ggcttcgctg tttttctcct
    62881 tcgcgggttg tggatgcatc gcgacacctc atagcgggga ttgtactccc gcaggccagc
    62941 gctggcgaca actctgggta agctggccgc ccgaagaaat tcggcaaatt cgctatcgct
    63001 ccgggggact tctccggcta cgccgttaat cggaaattga gcctgcgtgc gtggacggtt
    63061 cacagtgcgc tcagctcgag ttggggggcc gtagagccta tttctcgcgc gctgggcgcg
    63121 ctgcactcga aagtccgacg gccgactcca caggatctgg agtggccctg cggcccgtga
    63181 tctggtcgat gtgtatgcga tagaaaagcg catcggctgg ctggaaaccg ccccgcacct
    63241 gaccagccgc gtacgccgtt tgccaccaca acacccttct ttcgagccgg acttgggtct
    63301 gcagtcgcac gtctcggtac accggcgtgt cgggaagctc ctcgtagtgg ccgagcacga
    63361 cgacgctgga ccaatgaaaa tggctcacga cgtcgtcgac ctccacgcac accagcggat
    63421 ttgcacgcat ccactcgacc ttgcgtccca aagtggtgaa cccgtagaga tagtcgcggt
    63481 cataggcgaa atagatcgga acgatataag gctgatcctg gtgcacggtt gcgagcctgc
    63541 cgtactccgc ccgcgcaagc aggccacggc actcttcgcc gctcatctcc cgtactatca
    63601 tcgttgccct ccacgcttca taagacttgc cttgcacaaa ctctagcgga aaccttactg
    63661 gacgcacgtg atcggtgcga catgaggccg tgatcgcggt cacagaaagc tttcgcacat
    63721 cgggctgaac atgaactatg tcgttcaaga tactgcgcga acttgagaac ggagaattgc
    63781 tggaggtcgc caaccacggc gatttcgaaa aagccgaagc gctcgtgcga gcgttgaacg
    63841 aacactggcc cggagtctat ctgatcaggg aaatcgagaa cgaggaatct gaatgaggtt
    63901 cgcaggaatc gccgccgtat ggctcggcga tccttgcagt ggaatggcgc tatgacccga
    63961 ccgaaacccc cgaaggtcgc tcccgagcat gtcgaaatcg tcgcgcagtt ttcctaccat
    64021 gccttcgggt gtttcattag ccgttctttt gacgaagtcg taaggcaggt catacgagcg
    64081 ggctatagga cagcactaac cgccaagagt ttgagggacg aggtagaaga agggttgaac
    64141 agcgaggggg ccgagtagtc gtagtcctag ttccgtacaa gtcagggtgg cagacacgat
    64201 cccgctattg ttgatgcgtt tgcaattagc gggaacgtgc gaatttgaac ccctcagtgc
    64261 cagaatgccg aggactgaca tttgagaaaa aggtgggccg tttgaaccag cgaagacacc
    64321 tctcgcagta attccatcct tccgttagcg tcggtcgttt ctgagaactt cacgagggag
    64381 aagtgggacc gggacgattt tcggtatggg gagctcacac agctcgagac caccgcatga
    64441 gtggcaatgc aggagcaact gttgcaggtt gttcagagcg atgcgaagga agttgacggc
    64501 cgctgtccgc taaaggccaa aatccgggtt tccctcggct tcgctcggga tgatgtcctc
    64561 ccggtatggc tacctagtct gtttcgccaa ggatcttgtt ctcactgggt gattgcggac
    64621 cagaagattt cgaggagcgc aactccggcg aagcccactc cgatgtacgc cgccaaatag
    64681 accgccagaa aggcagcggc cgcgagcgaa cgtttcttgg ttagtttctc aggtgccgat
    64741 tcccagggcc gcatcactac gctgcgcgcg ggcggcggaa gaagcggaac aaaagcatcg
    64801 ttgcgaaggc tggactcgca ggtttcagtc atggatgcag tgtagaagct acatacgaaa
    64861 agaatccaat tcaaatttgc gttccaagca atcgcgatcg aagataggtc tcgcggaaat
    64921 ccagcgcggg cacttattca ataaatcgat tcaccgcata tatgaaacaa atatagtcca
    64981 gctttccttt cactcgtttt ccacttatag ttggtacccg cgcctacaaa ttccgtatgc
    65041 catccggttc gacgaacact tcactgagtc aagctgtgcg cccggtcgcc aaagtcctcg
    65101 tggcgacgac ggcgatgctc gcgttcatct cctattggcg agccgcagcc atcgtcctta
    65161 atgacctagg gtcctccgca ttctacgcgg gtgcgatagc cgagcacttc atcggcaaga
    65221 cggcgccctg gtttgtcctc gccattatgc tgttcgcctt tgccgtgcag gcgctctact
    65281 tcgaaagctg cgggatgttc gtgcgcggcg gcgtgtaccg cgtggtgaag gaggggatgg
    65341 gctcgacgct agcgaagttt tccgtctcgg cgctgatgtt cgactacatc ctgacggggc
    65401 cgatcagcgg cgtgtcggct gggctgtatt tggtcgggct gttgaacgaa cttttccagt
    65461 acgcgcactt gaagatcgcg ctgcccgtca acgccggggc ggccatcttc gccattatcg
    65521 tcacactcta cttttggtgg gagaacgtca aaggcattcc cgaatcgagc gagaaggcgc
    65581 tgcggatcat gtatgtcacc acggtgatgg tgatcatgat gatcgcgtgg tgcggttaca
    65641 cgctcatggt gcgcggcggg cacgttcctc cgtttcctca tatttcaaat ctggtctacg
    65701 cgccggattc gctgggatgg ctgaaacata cgtcgttgcc ttacacgatc ggattcatcg
    65761 gaatcatgat cggcctcggc cactcggtgc tggcgatgag cggcgaggag acgatggcgc
    65821 aggtctaccg cgagatcgag catcccaagc tccccaattt gaaaaaatcc ggattggtga
    65881 tctttcttta cagcctgatt tttacggccg gagtcgcgtt tttcgcggtg atgattattc
    65941 cggattcggt gcgagccaga ttctcggata acccgatcag cggcttggcg atgtatctcg
    66001 ttggaccgct ttcggcgcgc ctcgcattcc gcacgtttgt cgtggtggtt ggcgtgctga
    66061 tgctggcggg cgcggtgaac acggcgatcg ttggatcgaa cggcgtccta aatcgggtct
    66121 cggaagatgg cattctgccc gactggttcc ggcacccgca ccgccggttc ggcacttcgt
    66181 accgcatttt gaacctcgtc gtcgggcttc aactacttac tatcatcctc agccgcggaa
    66241 acattttcgt gcttggcgag gcctacgcat tcggcgtgat gtggagcttc gcgatgaagg
    66301 ggctggctgt ggttgtgctg cggtacaaac agcccggcca gcgggaattc aaggtcccgc
    66361 taaatccgaa aatcgccggc gtagaaatcc cgatcggcgt tgggctgatc acgctcgcgc
    66421 tcctggcgct gtgcttgatc aatttgttta cgaagcaggt ggctaccgtt tcgggaatcg
    66481 tatttacgat cgcatttttc gccgtcttcg agatttccga gaaaatgacg aagaagcagg
    66541 cgacgtcgca cgccgagtta gataagttta accttgcgca gtcgggagat ttgacgctgg
    66601 aaaatgttgg cgtcaagccc ggcaacgtcc tcgtaccggt gagcaactat tacgcgctgt
    66661 atccgctgga ggcggcgctg aagagagcga aacgcagaga ggccgaaatc gttgtgctgc
    66721 atgtccggct tctggggcgc gcggcttcgg gcgaatcgga tctcgcgccg gaccagctct
    66781 tcagcacgat tgaacaatcg ctcttcacga aagtgctggc gatggcagag aaagaaggga
    66841 agcccgtgcg gctcgccgta gccgccgcca atgacctgtg ggagggcatc ctgcgcacgg
    66901 ctgccaacct ggaatcgagt acggtggtcg ccggaagctc tgctaaagcg tcggttgcag
    66961 agcaggctcg agaaatcgga cttgcgtggg agcgaatgcc cgagccgcga ccccgagtgt
    67021 cgctcgaaat ttttgcgcca tccggaacgg agcaagtctt ttatctcggg ccgcacgcgc
    67081 cgcgattgac tcccaacgaa atcgacctgc tgcaccgaat ctggctcgat ctgagcgacc
    67141 ggttgccagg cgaagagatt caccaccacg atatcgttca tttcgcgctc agcgaagtcg
    67201 agcgcgagat cggcgagaag gagcgggacg gcgtgctgag gcgattgcgc gaacacctta
    67261 gcggcatcca gggacgccgg caaaacccct agtgttgctc cgatcgagcg cggcaacgat
    67321 gccgtagttt tcgctccagc ctcatctaga tgaaattcct cgtaaagaca agaagtggtg
    67381 catttttcct gcggtgcagc cagatcaaag aaagaggctg ctgaggcggc tgcaagtagt
    67441 taagatagaa ggagaaaagc ggcgcgcccg tagctcagtt ggaaagagcg tctgccttct
    67501 aagcagaggg tcgggggttc gactccctcc gggcgcgcca ctctaatccc tgccccgcct
    67561 tggatctaag ctctcggctc cgcaaagtgc gcaagtcgca gagaattgcc aagtgcatgc
    67621 ggtccggcga ggtgaaggac cttttcaact cgatgccggt cgagttcaag cccaactccg
    67681 tgatactcga cttcggcgga gcaccgcaag aaatcgcaaa cgatcacgcc tgagttttca
    67741 cctgcggcga accgcctacg gcgttcttga agaaaatcgg cgtcggatgc ggaatgagcg
    67801 acatcacgaa gcaagcagcg aagcgaaaca agcgatgact gcgagactcg gttcgcctgt
    67861 gctcgcaggc tcaccgatga acgcgtaaaa tgagaactcg gcaagactgg gagagaatat
    67921 tgacgtgatc tcgcgcttag cgattctgcc gttggttctc gcggcagcct ttgtgctcgg
    67981 ttgcagcaaa gagagccagc cttcgcagat gcctgcatca tcggccgccg ctcccgatgc
    68041 cagcagcgca accatgccat ccgctccggg ttcgagcgca cccgcttcag gcagcgatgc
    68101 tgaccagatt cgtcaggcgg tcatcgacca cgtgcgcaac gaccgcggcg tcaacatgtc
    68161 cgcgatggat atgagcgttg atttggtgaa cgtcaacgga gatcaagcac aagccaatgc
    68221 cacgtttcga gtcaagcagg gcggcgctac aatgaccatg gtctattctc tccaacgtca
    68281 cggaaatggc tggctcgtcg tcaatggcca gccaagtgat ggccaattcg tccatcctcc
    68341 aatggacagg acacactccg gagtgtcggc gagccccggc gctcccgaga tgcccgacgt
    68401 tcgagaattc ctaaaaactc acccgtcgac aaccggacag cccgaggcgc actgagcccg
    68461 gcgcaagggc ccggttccgc gatgggcgca ctcgtggcgg ttctcctcga cagcatttgg
    68521 ttacgctgag ataatcgcga caaacgcgca agagccaagc tgggctggtc tcggcggcgg
    68581 ctcttgcaag aatgaggata agacaagaat ggattcgtta atgacctcac cggttaagaa
    68641 ggcattgcgg ccgctcttgc ggacgggcat tttgtgctgt gtcgtggcga ccatgtgcaa
    68701 gccggctctc tcgatcacgc tggagagtcg caaacccgcg gaacatcggc tgcgcatgtt
    68761 taacacgcat accggcgagc aaattgatat cgtttaccgc cgaggaaatg actacgtcgt
    68821 agacgctctt gcgcaattgg actatttcct gcgagatcat cggactggcg atgtccacca
    68881 tttcgatccc cggctttatg acgttcttga acgactgacc gccgctgtcg gccgactggg
    68941 cgccgagatc gatgttattt gcggatatcg gacgccatgg agcaataact ttctccgtac
    69001 gcacagcccc ggagttgccg agcacagcct gcacatgcaa gccgaggcaa tcgacattcg
    69061 gatgcccggc gtcaacagcg aacggctgcg cgaggccgcc ctccatcttc atgagggagg
    69121 tgtcggctac tatccgcact ccaactttgt tcatgtggat gtgggccgcg tgcggcagtg
    69181 gtgcttcggc tgtaccggag agcagatgca cgccgactaa tgcggatagg gatactcgct
    69241 ccgcaaggct tcttccaatt tcgcgtcttg cccgtagata tcatcgtaga agcgcacaat
    69301 cccgtcttct agatctatcg ccgtaccgta aacaaccagc accggaatcg gatgcacgag
    69361 ttttacctca agtgtcgctt cgccattcat cgcggcgaga atgcggtccg ggtcccaacc
    69421 gggattgtcg cgcagtaccc aaccggccaa ttcttcaggc ttctccaacc ggatgcatcc
    69481 atgactgaag tctcgttggg atcgcgagaa tagtagtcgg gcgggcgtgt catgcatgta
    69541 gacgctgtac tcgttcggaa actgaaactt gatgagtccc agcgcgttgt tcgggccggg
    69601 cttttgccgc actgacaggc tcccggcgcc aatctgattc aggaggtgcc tgttgactgt
    69661 atccgtcgca acgacctccc cgcggccgtt cacaatctcc atgttttgtt ttgcgagata
    69721 ttcaggatcg tcctcgatcg cgggaacaat ctcctctcgc gcgatcgata cgggaatatc
    69781 ccaatacggt cggaagacca cggattgcaa gcggctcatg aaaattggag tttcatgatc
    69841 gtaggcgcgg cccacgacaa ccttcattgt gacggcgacg tggaacgcgt cgtcataagc
    69901 gcgcaacatg aatcctggga tgttgactac gattggcgct tcgccatact gcgccggcaa
    69961 ccaccgccaa cgttcaaggg tcaattcgat ttgcctcacg cgactttcca gcggaacatt
    70021 tagctcctcc agggtcttgg ggccgatgcg accgtccggc gtcaacccat ggcgctcctg
    70081 aaatctcctt actgcatccg cgagagcgga gtcgtatttt gtctcgtcgg aaggtatcgg
    70141 cgcatccgca gcaagatctc caactagctg cagaaactgc ctgaggcgcg gcgcgcttga
    70201 atacgattgg cctggtgcga tggctcgatt ggtagcaggg aagggttggc ccgtcttttc
    70261 ctttgccaat cggcggtacg tttccaaggc gcgtatcgtt cgttcatagc cagggtagga
    70321 tggttcgact cgcaagaggg ctgctcgcac gtctgctgcc ggaacaatct gttcgctgag
    70381 aaatttcggc aaatcgcatt ttctcttggc gatatttaca tcgatcccga acagatgcgg
    70441 atcgattcgg ccagtgtgca ggtcggagac atagtgcatc agagagacag taagcgcgac
    70501 gtcgaatcgc acggtgccgg cctcgtcggt ttggctggta ttcggctgca gccgggcgag
    70561 ccggtcggcc catcgcggcc cgtcatagtc gtctggggag aggcctttct cgtccgctcg
    70621 cgacaaagcc tcaatgacag cccgagcctg attagtagcc tgcatgtctc gaatccaagg
    70681 cagggcgtat cggtaagatt gatagaaggt tgcgatgtcc gcctgatagc cgcgaaattc
    70741 gggccacctg aggtcgtcga gccgcccgct ttcgatgatg gaacgcaaat acgcctcgcc
    70801 tgcggggctt agcggttgag caatgttgga gcgcgccacc ggggattgct gcgaccgcga
    70861 tatttcagcg cgtccaaggc tgcttgcaac gctgaaaagc aagaaaagga gcgctaccag
    70921 ggaccgccga aatcgtgttc ttgaacgtat gagcatcttg agacaactaa ttggtcttac
    70981 tccttcgctg aaccgtcaca tatagagcag gtgtcaaacc acatctgtga cacgaatcac
    71041 caatccgtgg cgtgcaaagg cgggtgcctc tcgtttcgct tgtctaccga cttatgacac
    71101 aactcacagt gtttggcagc gcgtttaaga aagatggggc gcggcggtat ggcagcaggt
    71161 gcccggtgac gatcaggagc gccgattgtt cagacggctc actgattccc tgttagcgaa
    71221 ggaggtcaaa cgttcgggga ccagcacatt gtgcgctcta ggttcgcgga ttatctggac
    71281 gcaacttccc ctgcgcggtg tgattctcgg aggcgagaat cgcgccctcc aagtagcagt
    71341 tcaggtcagg actttagaac ctgcccgcta gcgccactta tcgatcgacg cgctggtgac
    71401 gtagctgaag tcgccgccgg catagtacgt gttcgtctcg cctccaagca cgatgacctc
    71461 aatcgaagat ttcgggttgg gatcgggcgg cccgcccgga cggccgcggc ggtagaagcg
    71521 ctggaacgga atctcgccat cgcccagctt cagatacgaa gcgtaaggct cgacgcccgc
    71581 ctgcgcctgc ttcaattcat tcttgtgcgt gtcccagtag agccacgccg gcgtcttcga
    71641 ttccttcgac caatagttgc ttaattcttc cttcgagttg aatccctttc cctttgcttc
    71701 gcgggcgatt gttggatcga gcagcacggt ggcagcgctg gtgccgaacg agaagaaatg
    71761 cgtcagccag ttcttgatga gttcgtggta gggcatgttg gggaaccagg cgacgttgtt
    71821 caggctccag ccgctgaaga tgctgaccac gctatcgtca ggcttgaagc ccttttgcac
    71881 gtggaagggt ttccagccgt ccggaagctt gttttccgtt tccgcgatgc agatattgtt
    71941 gtagttgaag ttggatccca gagtgcccat gtagctgagc ccaggcattc cgcagccgcc
    72001 gaggtttttc gaaaggatcg tccatgcgcg gccaatcaca gcattgggct cggcgaacgg
    72061 acccatcgcg ccgatgccgt agttcatctt gatctggtcg gagatggggc cattcagcag
    72121 cagcatgcgg gagaatgagg acgtggaact gaacagggcg ggctgttcgg tcgacgcaat
    72181 ggccaaaatg acgggcagat actccggggt cgcgccggcc atgacggcat tgatggccac
    72241 gtgcctgacc gtgaaggacc acgacggctg agctccgcgg gcggcgctca acgtgccgat
    72301 aacctcgtca ggcttatggc tcgtgccctt gagcatcgct tgcacctttt cctcggtggg
    72361 aaggatgatg ggcatgaagt cagtcatgtt gttatcgagg tagatccgct ggagatcgtt
    72421 cggcgtgccc acgtaggtag ccgggccgat gtccggggta acctggccgc tcttttgctc
    72481 gtcggccgtg agcggcttgg tgagcgcgtc catgatttcc ggcatgagcg gcttattcgt
    72541 gacggggtcg ttgccaccga gaacggcata catttcctcg tccgtcttgt tggtcatggg
    72601 gtgagggagg aagcagatgc gctcgccggg catgccgcgg ttcgcggcgt tggtcttggc
    72661 gagatccttg aaggcgatcg taaccagggg ggccgtggga aggcccattt tctcaacttg
    72721 tgcgcagtga ccgacggtcg ctggtgtgca ggtgcttcaa tg
//
LOCUS       DASPUL010000227         3847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_41036, whole genome shotgun sequence.
ACCESSION   DASPUL010000227 DASPUL010000000
VERSION     DASPUL010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41036"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VEJ00_05675"
     CDS             complement(<1..697)
                     /locus_tag="VEJ00_05675"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HXX00683.1"
                     /translation="MSAGTQQIVAPWTPAPPQLVMAYSVCRGITRSAAKNFYYAFLVL
                     PRRKREALCAVYAFMRRCDDICDDTSLSLPERRQKLESWLEALHRVQQGQPTDDPILL
                     ALTDAQRRFTIPAGLLDELAHGTAMDVEQFEAAAQASATPGLTVEYKTFEDLRLYCYR
                     VASVVGLVCIHVFGYRDPAAEPLAESCGLAFQLTNIIRDVKEDASMGRVYLPEEDLVK
                     FGLSAAELLAKPDP"
     gene            complement(694..1665)
                     /gene="hpnC"
                     /locus_tag="VEJ00_05680"
     CDS             complement(694..1665)
                     /gene="hpnC"
                     /locus_tag="VEJ00_05680"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HXX00684.1"
                     /translation="MSVNNDLIHVSQLATQTAPPGWSRLPADYAIPAAPPTLEEARAF
                     CAHLARTHYENFSVATWFLPRRLRQHFYNVYAYCRISDDLGDEVGDAAASLELLDQWQ
                     AELDASYGGAPRHPVFVALAETVREFDIPKHEFSDLLTAFRQDQTVTRFETFDDVLGY
                     CRYSANPVGHLVLYLCGYRDAERQQLSDFTCTALQLANFWQDVSVDFAKGRIYLPLED
                     LRRFGVAEDDLVCSRNTPAFCLMMKFEVGRAHDWFRRGLPLIGKVDRELAIDLELFSR
                     GGQEILRAIARQGYAVLGNRPAISKRRKLLLVARAAMGKLLRPGAPR"
     gene            complement(1775..2824)
                     /locus_tag="VEJ00_05685"
     CDS             complement(1775..2824)
                     /locus_tag="VEJ00_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXX00685.1"
                     /translation="MNGKMTAAVLYGKEDLKIEKVPIPRVGDGEVLVKVQVALTCGTD
                     LKVFQRGYHARMIVPPALFGHELAGVIEEVGADVTGFKKGQRVVALNSAPCQMCFYCS
                     KHQENLCEDLLFNNGAYAEYIRVPRRIVEANMLVIPPNVSFEDAAMTEPLACVLRGLH
                     ETGVEIGDTVAILGGGPIGLMFVQVARAIGCNVISVVKRDGQVEAARQMGAHEIVQTS
                     KVPNVVEAVRALSPQKRGCDVVIEAVGKPEAWEWAVDMVRKGGTVNFFGGCASGTKVQ
                     LDTNRLHYSEITLKATFHHTPETVRRAFGLIAAKKIRPTDYITGEAPLSRLQQVLRSM
                     LNRGSDIKTAIIPGH"
     gene            complement(2865..>3847)
                     /locus_tag="VEJ00_05690"
     CDS             complement(2865..>3847)
                     /locus_tag="VEJ00_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXX00686.1"
                     /translation="EMLVRVHSCGVCGTDLKKISTGSHSAPRIFGHETSGVVAAVGAG
                     VLDYRPGDRVVVFHHIPCRECFYCRSKTFAQCATYKKVGCTAGFEPSGGGFAEYVRVM
                     DWIVEHGTVRIPDGVSYEQACFVEPINTCMKGVEALRLEQGETVLTIGQGPIGIMLSV
                     LAKRTGVRLITSDLYAERLRIGTRFDLELTIDASRTKVVERVRELTEGRGADAVILAV
                     GGNSLIRTAMDAARPGGRILLFAQTQHGEATIDPAAICVDEKTLVGSYSASVDLQEES
                     IRFVMNREMDLEQLISHRFPLAQSTAALDLAAHPQPSSMKIMIQPGSAWA"
BASE COUNT          746 a         1213 c         1166 g          722 t
ORIGIN      
        1 ccggatccgg cttggccagc agctccgcgg ccgagaggcc gaacttcacg agatcctctt
       61 cgggaagata tacccggccc atgctcgcgt cttccttcac atcgcggatg atgttggtga
      121 gctggaaggc cagcccgcag ctttctgcca aaggctcagc cgccgggtcg cgatagccga
      181 aaacgtggat gcacaccaga ccgaccaccg acgctacgcg atagcaatac aggcgcaggt
      241 cttcaaaggt cttgtactcg acggtaagtc cgggggtcgc gctggcttgc gcggcagcct
      301 cgaactgctc cacatccatg gcggtgccgt gagcgagttc gtcgagcaat ccggcgggga
      361 tggtgaatcg tcgctgcgcg tcggtcagcg ccaggagaat cggatcgtcc gtcggctggc
      421 cctgctgcac gcgatgcaga gcctcgagcc aggactccag tttctggcgg cgttccggca
      481 ggctcagcga ggtgtcgtcg caaatatcgt cgcagcgccg catgaaggcg tacaccgcgc
      541 acagtgcctc gcgcttgcgg cgcggcagca cgaggaaggc atagtagaaa ttcttggccg
      601 ccgagcgcgt gatgccccgg cacacggaat aggccatcac aagctgcggc ggagccggag
      661 tccagggggc aacgatttgc tgtgtgcctg cgctcatcgc ggtgctcccg gacgcagcag
      721 ctttcccatg gctgcgcgcg ccaccagcag caacttgcgc cgcttcgaaa tggcgggacg
      781 atttcccaac accgcatagc cctggcgcgc gatcgcgcgc agaatctcct ggccgccccg
      841 gctgaacagt tcgagatcga ttgccagttc gcggtcaacc ttgccaatga gcggcagccc
      901 acgacggaac cagtcgtgcg cacgcccgac ctcgaatttc atcatgaggc aaaaagcggg
      961 agtgttccgg ctgcagacca ggtcatcttc cgcgacccca aagcggcgca ggtcttcgag
     1021 cggcagataa atgcgccctt tggcgaagtc gacggaaacg tcctgccaga agttggcgag
     1081 ttgcagagcc gtgcaggtga agtcggaaag ctgctgccgc tcggcatcgc gatagccgca
     1141 gagataaagc acgagatggc cgactggatt cgccgaatag cggcagtagc ccagcacatc
     1201 gtcgaaagtt tcaaagcggg tgacagtctg atcctggcga aaggcggtca gcaggtcgga
     1261 gaattcgtgc ttcggaatat caaactcgcg cacggtctcg gccagcgcca cgaacaccgg
     1321 atgtcgggga gcgccgccgt agctggcatc gagttcggcc tgccactggt cgagaagctc
     1381 cagcgaagcc gcggcgtcgc cgacctcgtc gccaagatcg tcggagatac ggcaatacgc
     1441 gtagacatta taaaaatgct ggcgcagccg ccggggaagg aaccacgttg ctaccgagaa
     1501 gttctcgtag tgggtgcgcg cgaggtgggc gcagaatgcg cgggcttctt ccagcgtggg
     1561 aggcgctgcc gggatggcgt agtcggcggg gagacggctc cagccgggcg gcgcggtttg
     1621 ggtggcgagt tggctcacgt gaatcaggtc attgttgacg gacaattttt gattgttgag
     1681 tgagaatcct gcagttcacc ctcgaggact tttcaatcaa caatcagcaa tcaagaatca
     1741 acaatatgct ttcggatgcg ggctcgttgc gccttcagtg tcccggaata attgcggtct
     1801 tgatgtcgct gccgcggttc agcatggagc gcagaacctg ctgcaaccgg gagagcggcg
     1861 cctcgccggt aatgtagtcg gtgggacgga tctttttggc ggcgatcagg ccaaaggcgc
     1921 ggcgcacggt ctccggagtg tggtggaacg tcgccttgag cgtgatctcg gaatagtgca
     1981 ggcgattggt atccaactgc accttggtcc cgctggcaca tccgccgaag aagttcacgg
     2041 tgccgccctt gcgtaccatg tcgacggccc actcccaggc ttcgggcttg cccaccgcct
     2101 cgatgaccac atcgcaaccg cgtttctgcg gagagagcgc tcgcaccgct tcgaccacat
     2161 tgggcacctt ggaggtctgc acgatctcat gggcgcccat ctgccgggcg gcttcgactt
     2221 gtccgtcgcg cttgaccacc gatatcacat tgcagccgat ggcgcgcgcg acttgcacga
     2281 acatcagccc gatcggcccg ccgcccagga tggcgaccgt atccccgatt tccactccgg
     2341 tttcatgcag gccgcgcagc acgcacgcca ggggttcggt catggcggcg tcttcaaagc
     2401 tcacgttggg cgggatcacc agcatgttcg cttctacgat gcgccgcggc acgcggatgt
     2461 attccgcgta ggctccgtta ttgaacagca gatcttcgca gagattctcc tgatgcttgg
     2521 agcagtagaa gcacatctgg cacggcgccg agttcagagc gaccacccgc tggcccttct
     2581 tgaagccggt gacgtcggct ccgacctctt cgatgactcc ggccagttcg tggccgaaca
     2641 gagccggggg gacaatcatg cgcgcgtggt agccgcgctg aaatactttc aggtccgtgc
     2701 cgcaggtgag ggccacctgc accttgacca gcacttcgcc atcgcccacg cgagggatgg
     2761 gaactttttc gatcttgaga tcttctttgc cgtacaggac ggccgctgtc atttttccgt
     2821 tcactgccag ttccttcccc ttactccccg aactgcgttt ttcctcaggc ccatgcgctt
     2881 ccgggctgga tcatgatctt cattgaggag ggctgcggat gagccgccaa atccagtgcc
     2941 gccgtactct gggcaagcgg aaagcggtgg ctgatcagct gttccaggtc catctcccgg
     3001 ttcataacaa aacgaatcga ctcctcctgc aaatccaccg acgcgctgta ggaccccacc
     3061 agggttttct cgtcgacgca gatggcagcg ggatcaatcg tcgcctcccc gtgctgagtt
     3121 tgcgcaaaca gcaatatccg cccaccggga cgggccgcat ccattgcggt gcgaatcagg
     3181 gagttgccgc cgaccgccag aatcacggcg tcggccccgc gcccctcggt cagttcgcgg
     3241 acacgttcca ccaccttcgt ccgcgaagca tcaatcgtga gctctaagtc gaaacgtgtc
     3301 cctattctaa gcctctcggc gtataaatcg gaagtaatta agcggacccc cgtccttttg
     3361 gccaatacgc ttaacatgat accgatgggg ccctgcccga tcgtcaacac agtttcgccc
     3421 tgctccagcc gcagagcctc gactcccttc atacacgtat tgatcggctc gacgaagcag
     3481 gcctgctcgt aggaaacgcc gtccgggatg cggactgtgc cgtgttccac gatccaatcc
     3541 atcacccgca cgtactcggc gaagcccccg cccgagggct cgaatccagc cgtgcagccg
     3601 actttcttgt aggtggcgca ctgggcgaag gtcttgctcc ggcagtaaaa gcactcgcgg
     3661 cagggaatgt gatgaaagac caccacgcgg tcaccaggcc gatagtccag cacaccggct
     3721 cccaccgcgg ccacaacacc cgacgtctcg tgcccgaaga tgcgcggcgc cgagtgcgag
     3781 cccgtcgaga tcttcttgag atccgtcccg cagacgccgc aggaatgaac ccgcaccagc
     3841 atctccc
//
LOCUS       DASPUL010000228         6511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_998400, whole genome shotgun sequence.
ACCESSION   DASPUL010000228 DASPUL010000000
VERSION     DASPUL010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6511)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6511
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998400"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VEJ00_05695"
     CDS             complement(<1..181)
                     /locus_tag="VEJ00_05695"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXX00687.1"
                     /translation="MADVRRGLIIVNTGPGKGKTTAAMGTALRAVGQGMRVLMLQFLK
                     GSWHYGELDAVKAFGD"
     gene            485..961
                     /locus_tag="VEJ00_05700"
     CDS             485..961
                     /locus_tag="VEJ00_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXX00688.1"
                     /translation="MLNKCAALLLLACAALPAFSAQHVAVHPRPPIDSAEQATIREIV
                     DMERQAREASLRRDADFSQRTLAEDYVAITPLGQVTTKKDTLSARKSGQLRYESIDIS
                     DMVVRVYGDTAVVTARADVKGHQLGEDFSGPYRYTRVWVRHGGHWQAVSYQATVTQ"
     gene            complement(1033..1485)
                     /locus_tag="VEJ00_05705"
     CDS             complement(1033..1485)
                     /locus_tag="VEJ00_05705"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HXX00689.1"
                     /translation="MKIKIVWIGKTKEPAIQALTEEYLKRISRYTQVEGQALRDEAPL
                     LESCGRAASGKVAGKATLVLMDAQGKEYTSVQFAKFLGDYQERNPLPLIFAVGGADGF
                     SPEARAAAHHVVSLGKMTLAHELARVVLLEQLYRAFTILKGHPYHSGH"
     gene            complement(1549..1980)
                     /gene="rsfS"
                     /locus_tag="VEJ00_05710"
     CDS             complement(1549..1980)
                     /gene="rsfS"
                     /locus_tag="VEJ00_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXX00690.1"
                     /translation="MKKSELKKQVSAAIQACLEKKAEELSILEMEKGSGAFTDYFVLC
                     SGTNPRQIQAIADEVEMRLKAASLRPAHIEGYNQAEWVLIDYIDFVVHIFSEKARRFY
                     DLERLWKTAKHLQPADLKAAPRTRKATTARADTRKRKKKTA"
     gene            complement(2068..2199)
                     /locus_tag="VEJ00_05715"
     CDS             complement(2068..2199)
                     /locus_tag="VEJ00_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00691.1"
                     /translation="MSQNHYVTKPKRRAKSGKRKDPSAATMAKLRQRAAAEAKPPGK"
     gene            complement(2299..2991)
                     /gene="nadD"
                     /locus_tag="VEJ00_05720"
     CDS             complement(2299..2991)
                     /gene="nadD"
                     /locus_tag="VEJ00_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HXX00692.1"
                     /translation="MNIGLFGGTFDPVHRGHLALARAAQQQYKLHRVYLVPANIPPHK
                     QKHPLTRFLHRFAMLVLATAEEKSFVPSLLEAPDDSEFGAYKNRQPKPNYTIDTVRRL
                     KESFKASDKLFLLIGMDAFADIAKWHQAEALFRECEFVVASRPGYSLADVANALPESL
                     RPRPEVTKPFQKQKASGELVLPGATLHLLGDLNQPASATAIREAAAAGKPLGRFVEAT
                     VAEYIRKMGLYK"
     gene            3354..5624
                     /locus_tag="VEJ00_05725"
     CDS             3354..5624
                     /locus_tag="VEJ00_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXX00693.1"
                     /translation="MRVTLPLLRIPPARAPILRYGVAVLSTALALIPTVMLADVSESR
                     LALFGVAVMMSAWYGGWKPGLVATAFALTVSAYFSLAGEHSPAQFRTAIVRLSLFFFI
                     AMLICWLNGALRAAQENLRRSELNFRSLVTNAPYGICRCDSTGKILDVNPALIETLGY
                     ASAAELIGQHIYSLYADTDRWFDLADFFRSSTPFKGLTAEWKRKNGTTTVVRVSGRSV
                     SNGRETGLVFELFTEDVTERRVLEQQLRQSQKMEAVGRLAGGIAHDFNNLLMVISGYS
                     EFLLERLGAEPQLRGPAQEIASAAERASGLTRQLLAFSRKQMLAPRIVNLNDVASENL
                     KMLTRMIGEDIDLVLVPGTNLWPVRADAGQIDQVIMNLAVNARDAMPSGGKLTIETSN
                     VTLDEDYARFHAPLRPGEYVMIAISDTGAGMDAETQSHIFEPFFTTKGTKGTGLGLST
                     VYGIIKQSGGYIWVYSELGKGTTFKIYLPRVVAAGEPVVQVAAPVEVSKVEPGTETIL
                     VVEDETNLRYLARQFLEKQGYKVIEAADGAVAMQIAVAHEGVIHLLLTDVIMPGMNGR
                     ELAQRISEIRPNIKILYMSGYTENVVGHNGMLDAGVRLLQKPFNLRDLRSKVREVLDA
                     TPTPPEVAMSTQFAEPSRTVTSRENPPSRAQRFHLHLPLRYRRLGEKQWHEGKTENIS
                     RSGLLFQGDEMLQPSSQLEINLVLPTEIAGVSSTEVVCRGEVVRTVEPKGTTLNPALA
                     ARILQYHFQHGPLPRA"
     gene            complement(5726..>6511)
                     /gene="obgE"
                     /locus_tag="VEJ00_05730"
     CDS             complement(5726..>6511)
                     /gene="obgE"
                     /locus_tag="VEJ00_05730"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXX00694.1"
                     /translation="LKVPVGTILYDADSGERVHDFARPDERVVIARGGRGGRGNARFA
                     TSVHQAPREHEAGRPGEERVYRLELKLLADVGLVGYPNVGKSTLISRISAARPKIADY
                     PFTTLEPNLGVVAVGDAKDEISFVVADIPGLIEGAHTGAGLGTQFLRHIERTRLLVHL
                     VDVSDSSGRDDVVKDVEVILGELGSFGAHLEEKPMIMAASKMDAVNKDKLAALKKYCR
                     KKRLKLFEISAVTGKGIEDLKYAMAEEVEEMRAKDLQAVEGKP"
BASE COUNT         1215 a         2064 c         1885 g         1347 t
ORIGIN      
        1 tgtcgccgaa ggcctttacg gcgtcgagtt cgccatagtg ccaggagccc ttcaggaact
       61 gcagcatcag cacccgcatc ccctggccca ccgcgcgaag ggcagtaccc attgccgcag
      121 tggtcttgcc cttccccggc ccggtattca cgatgatcag accgcgacgg acgtcagcca
      181 tgccgatcgg aggtttgatt ctactagaag cggcccggag cagtcccaca atgattcaat
      241 ctgagactcg agctcgcctc catgaaggac gagacgggat gcgagaaaaa aggctgaaca
      301 cggcacgttc tttcccgttc ataaccttcg aggggatgct ttccagtcac ttgtggcatt
      361 ctgttacgaa tcacagaggg aaatgttaag cttggggaac ttttccacgg aactcttgca
      421 tccaagactg cgtagctcgg tcaggcggat ctctgtcccc tcgcagaatg cggaggtctc
      481 actggtgctc aacaagtgtg cagcgttatt gttgctggca tgcgcggcct tgcctgcctt
      541 ctcggcccaa catgtagcgg ttcatccccg gcctccaatc gattccgcgg agcaggcaac
      601 catccgggaa atcgtagata tggagcgtca ggcccgcgag gcgagccttc gccgcgatgc
      661 cgacttttcg caacgcacct tggccgagga ctatgttgcc atcaccccgc tgggccaggt
      721 caccacgaag aaggacacgc tttccgcccg caaaagcggc caattgcggt atgagtcgat
      781 cgacatcagc gatatggtcg ttcgcgttta cggcgacact gcggtggtaa ctgcccgggc
      841 cgacgtgaaa ggccaccagt taggcgagga cttcagcggc ccttaccgct atacccgggt
      901 ctgggtgcgc cacggcgggc actggcaagc ggtgagctac caggcaaccg tgacccaata
      961 gccggtcttt tcggattctc attcaacaaa ctgcatgtaa caaatctgga atgcagaggc
     1021 gcaggtaggc agtcagtgcc cggagtgata cgggtgccct ttgagaatcg tgaacgcgcg
     1081 atacagttgt tccagcagca ccacgcgcgc cagttcgtgt gcaagcgtca tcttccccag
     1141 tgagactacg tggtgcgccg cagccctggc ctcggggctg aatccatccg ccccgcccac
     1201 ggcgaagatc agcggcaggg gattgcgctc ctgataatct cccaggaact tggcgaactg
     1261 cacggaggtg tactccttgc cttgtgcatc catgagcacg agcgttgcct tcccggcgac
     1321 cttccccgaa gccgcccggc cgcaggattc aagcagcggc gcttcgtcac ggagtgcttg
     1381 gccttccacc tgcgtgtagc gtgaaattcg ctttagatac tcctcggtca gcgcctggat
     1441 cgcaggctcc ttcgtctttc cgatccacac gatcttgatc ttcacggaga gctaatgtga
     1501 tatcgcttgc cggaattgga gccgtctgaa cccgcgccga tcggagcctc aggccgtttt
     1561 cttctttctt ttcctggtgt cggcccgcgc ggttgtcgcc ttccgcgttc ggggcgccgc
     1621 tttcaggtcg gctggctgca gatgcttcgc cgtcttccac agccgttcca gatcatagaa
     1681 ccggcgcgcc ttctcggaga agatgtgcac cacgaagtcg atgtagtcga tcagcaccca
     1741 ttccgcctgg ttatatccct cgatgtgcgc cgggcgcagg ctggcggctt tgaggcgcat
     1801 ttccacctcg tcggcaatcg cctggatttg acgcggattg gtgccgctgc acaggacaaa
     1861 gtaatcggta aacgctcccg atcctttctc catctcaagg atgctgagtt cttcggcctt
     1921 tttttccaga caggcttgaa tggcggcaga aacttgtttc ttgagttcgc tcttcttcat
     1981 tcagacttgg gggtggaggt caatcttcct tgtcagttcg attgtcaatt gtagccgacc
     2041 tggtttccgc cggctcgcgg gttgctgtca cttaccgggt ggtttcgcct cggccgcggc
     2101 acgttgccgg agctttgcca tggtggcagc agagggatct ttgcgcttgc cgctttttgc
     2161 ccgccgctta ggcttggtga cgtagtggtt ttgagacatg ctgaatctcc ttcttatctt
     2221 tcgatgtcag ggggcgttac gtgaaacagg cagtcaccat gataaccgga ggcacgcgat
     2281 ttgtgggttg tggcaggact atttgtacag ccccattttc cggatgtact cagcgaccgt
     2341 cgcctccaca aaacgcccga gcggcttgcc ggcggcggca gcctcgcgaa tcgccgttgc
     2401 cgaggcgggt tgattcagat ccccgagcaa gtggagggtc gccccgggca ggaccagctc
     2461 ccccgaagcc ttctgcttct ggaagggctt tgtgacctcc gggcgcggtc gcaggctctc
     2521 gggcagcgcg ttggccacgt ccgccaggga atatccgggc cggctggcca cgacgaattc
     2581 gcactcgcga aacagagcct cggcctgatg ccacttggca atgtcggcga acgcgtccat
     2641 tccgatcagc aaaaaaagct tgtcgctggc cttgaacgac tccttcagcc gtctcaccgt
     2701 atcgatcgtg tagttcggct tgggctggcg gtttttgtag gcaccgaact cgctgtcgtc
     2761 gggagcttcc agcagcgaag gcacgaatga cttctcctcg gccgtggcca gaaccaacat
     2821 ggcaaagcgg tggagaaagc gcgtgagcgg atgtttttgc ttgtgcggcg gaatgttcgc
     2881 cggcacaagg tacacgcggt gcagcttgta ctgctgctgt gccgcccgcg ccagcgccag
     2941 atgcccgcgg tgcactgggt cgaaggttcc accgaaaagc ccaatgttca tggatgttgt
     3001 cagaagcgtg agcgaacgag cagtttactc tctccgctcc caacagacat agcctcagca
     3061 agctgcgcct tgctttgcgc ccctttgcgg attttcttcg cgacctttgc gatcaaaggc
     3121 ttttgccgca aagttcgcaa aggaaatgcc gcaaagacgc caagggcggc ggaagtctcc
     3181 aacagattca cgacccgatt cgcactgcca ttgtcctgcc ggacactgtc ctgaaggccg
     3241 aggagacggt cagtgacgtg gaaccagtct gatcaccccc tcgccccgct gcacgctcca
     3301 aaatgaactc ctgtaactaa cgggctttcc cttttcggaa ctaggatcgt tacatgcgag
     3361 taaccctccc cctactccgc ataccgcccg ctcgcgcccc cattctccgc tatggggtgg
     3421 cggtgctgag caccgccctg gccctgatcc ccacggtcat gctggccgac gtgtcggaga
     3481 gccgcctggc tctgttcggc gtggcggtga tgatgagcgc ctggtacggg ggatggaagc
     3541 ccggcctggt cgccaccgca ttcgctctga ccgtaagcgc ctatttttcg ctggccgggg
     3601 aacacagccc ggcgcaattt cgcaccgcca tcgttcgcct gtcgctgttc ttcttcatcg
     3661 ccatgctcat ctgctggctc aacggcgcct tgcgcgccgc ccaggagaac ctgcgccggt
     3721 ccgagctcaa cttccgttcg ctggtgacca acgcccccta tggcatctgc cgctgcgatt
     3781 ccaccggcaa aatcctcgat gtgaaccctg ccctcatcga aaccctgggt tatgcctcgg
     3841 ccgcggaact gatcggacag cacatctaca gcctctacgc cgatacggac cgctggttcg
     3901 atctcgccga cttcttccgt tcgtccacgc ccttcaaagg cctgaccgca gagtggaagc
     3961 gcaagaacgg cacgaccacg gtggttcgcg tttccggccg ttcggtgagc aacggccgcg
     4021 agaccggcct ggtgttcgag ctgtttaccg aagatgtaac cgaacgccgc gtcctcgagc
     4081 agcaactgcg ccagtcccag aagatggagg ccgtcggccg cctcgccgga ggcatcgccc
     4141 acgacttcaa caacctgctc atggtgatct ccggctattc ggaattcctg cttgaacgcc
     4201 tcggggcgga gccgcaactg cgcggccccg cacaggagat tgccagcgcc gccgagcgcg
     4261 cgtcagggct gacccggcaa ctgctggcct ttagccgcaa gcagatgctc gcgccccgca
     4321 tcgtcaacct taacgatgtg gccagcgaga atctgaaaat gctgacccgc atgatcggcg
     4381 aggacatcga cctggtcctg gtgcccggca ccaatctctg gccggtgcgc gccgacgccg
     4441 gacaaatcga ccaggtcatt atgaacctgg cagtgaatgc ccgggacgcc atgccttcgg
     4501 gaggaaagct gacgatcgag acttcaaacg taaccctcga cgaagactat gcccgcttcc
     4561 atgctccgct gcgccccgga gagtacgtga tgatcgcgat cagcgacacc ggcgccggca
     4621 tggacgccga aacccaatcg catatctttg agcccttctt taccacgaag ggaacgaaag
     4681 gcacgggtct cgggctgtcg acggtttacg gcattatcaa acagagcggg ggttacatct
     4741 gggtttacag cgaactaggt aagggcacca cgttcaagat ctatctgcct cgggtggtcg
     4801 ccgccggcga gccggtggtg caagtcgccg cgccggtcga ggtttccaag gttgaacccg
     4861 gcacagaaac gattctcgtg gtcgaggacg agaccaacct gcgctacctc gcccgccagt
     4921 tcctggagaa gcagggctac aaggtgatcg aagccgccga cggcgccgtg gccatgcaga
     4981 tcgccgtggc gcacgagggc gtcatccacc tgctgctcac cgacgtcatc atgcccggca
     5041 tgaatggacg cgaactggcg cagcggattt ccgagattcg acccaacata aagattctgt
     5101 acatgtccgg ctacaccgaa aacgtagtcg gccacaacgg catgctggac gcaggcgtgc
     5161 gcctgctcca gaagcccttc aaccttcgcg acctgagaag caaggtgcgc gaagtgctcg
     5221 atgcaactcc aactcccccg gaggtcgcca tgtccacgca gttcgccgaa cccagccgca
     5281 ccgtgacgtc gcgggaaaac ccgccttcgc gcgcgcaacg attccacctg cacttgcctc
     5341 tgcgctatcg ccggctcggc gaaaagcagt ggcatgaagg aaaaaccgaa aacatcagcc
     5401 gctcgggatt gctattccag ggcgatgaaa tgctgcagcc cagttcgcag ctggagatca
     5461 acctcgtgct gcccacggag atcgccgggg tttcttctac tgaagtggtc tgccgcggag
     5521 aggttgtgcg gacggtcgag ccgaagggaa ccacgctgaa tccggcgctg gccgcgcgca
     5581 ttctgcaata tcactttcag cacgggccgt tgccgcgggc gtagctttca taacccggtt
     5641 ctgtggatca ggggttgatc tttcctggca gtggaaaagg cggcctgatc aagccgcctt
     5701 tggttttctt agcgtccttt gcggtctaag gttttccttc gaccgcctgc aagtccttcg
     5761 cgcgcatctc ttcgacctct tcggccatgg cgtatttcag atcctcgatc cccttgccgg
     5821 tcacggctga gatctcaaac agcttcagtc ttttctttct gcagtatttt ttgagtgcag
     5881 ccagcttgtc cttgttgacc gcgtccatct ttgacgcggc catgatcatc ggcttctctt
     5941 ccagatgcgc tccgaagctg cccaactcgc ccaggatcac ttcaacgtcc ttgaccacgt
     6001 cgtcgcgccc gctcgagtcg gagacgtcta caagatgcac cagcagccgc gtgcgctcga
     6061 tgtgccgcag aaactgggtg cccagcccgg ccccggtgtg cgccccttcg atcagcccgg
     6121 gaatatcggc cacgacgaag ctgatttcat ctttggcgtc gccgaccgcc accacaccga
     6181 gatttggctc gagcgtggta aacggatagt cggcaatttt gggccgcgcc gccgaaatgc
     6241 gcgagatcag cgtcgacttg cccacattcg gatatcccac cagcccgaca tcggcgagca
     6301 gcttgagttc cagccggtag acgcgttctt cgcccggccg tcccgcctcg tgctcgcgcg
     6361 gggcctggtg cacggaggtc gcgaagcggg cattgccgcg tccgccgcgg ccgccgcgcg
     6421 cgatcaccac gcgctcatcc ggccgcgcaa aatcgtgtac gcgctcgccg gaatcggcat
     6481 cgtagaggat cgtccccacc ggaactttca g
//
LOCUS       DASPUL010000229         3794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1686097, whole genome shotgun sequence.
ACCESSION   DASPUL010000229 DASPUL010000000
VERSION     DASPUL010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686097"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VEJ00_05735"
     CDS             complement(<1..184)
                     /locus_tag="VEJ00_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00695.1"
                     /translation="MRKSTLRYALLAGIASFALACLPVSAVAQRGGGGHGGGGGGFHG
                     GGGWGFHGGGGGGYGGS"
     gene            complement(378..1043)
                     /locus_tag="VEJ00_05740"
     CDS             complement(378..1043)
                     /locus_tag="VEJ00_05740"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HXX00696.1"
                     /translation="MANLFELPRYIAVEGPIRVGKSTLAQILGERLNAQRVMEPDSNP
                     FLDAFYEGERGAAFQTQFAFLVRRFEALQALDVGPRSRRVVVADFLFEKDKLFACLNL
                     SDQELDTYNRYYNYFREQLPTPDLVIYLQATPAVLKKRLKKKNAPGERAVSDEYLEEV
                     VKAYEHFFFHYTSSDLLIVNTSEIDFVDRNEDLRELLRKVSEPIKGTQYFLPLGGQEA
                     VGA"
     gene            1216..1938
                     /locus_tag="VEJ00_05745"
     CDS             1216..1938
                     /locus_tag="VEJ00_05745"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXX00697.1"
                     /translation="MLGALLAGTTAVAAVAAGYQSMSPTGQWYGRTFIGLAPGTKQLA
                     LTYDDGPNDPHTLRLLELLARHNVRATFFLIGRYVQQCPEIAREIAKAGHVIGNHTFT
                     HPLLTLNSNAEIRRQLLDGQSALHDAIGEHSNLFRPPFGGRRPAVLRIARELGLEPVM
                     WNVTGYDWNAPPAAVIERKVARQIRGGDVILLHDGGHKQMGADRSQTVLASEHMIARY
                     KASGYEFVTIPEMMGISASRRG"
     gene            complement(1966..3273)
                     /gene="hemL"
                     /locus_tag="VEJ00_05750"
     CDS             complement(1966..3273)
                     /gene="hemL"
                     /locus_tag="VEJ00_05750"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HXX00698.1"
                     /translation="MPSTTSRKIEQSRRMQQRAESMIPGGVNSPVRAFRSVGGDPLFV
                     VRGKGSHIWDADENEYIDYIGSWGPLILGHADAGVLDAIVGAACNGTSFGASTPSEAD
                     LAELVLAAFPHMQKVRFVSSGTEATMSAIRLARAYTKRKYIVKFEGCYHGHADALLVK
                     AGSGVATLGIPGSAGVPEEFIQFTVALPFNNLSAVEHAFQKFKHQIACVIVEPVVGNM
                     GCVPAADDYLVGLRLITERERAVLIFDEVMTGFRVAFGGAQELYNIKPDLTTLGKIIG
                     GGLPVGAYGGPNEIMDLVAPLGPMYQAGTLSGNPLAMAAGCAMLKQLRDRKGEIYPRL
                     EKLSGQLVEGVAAAAKDAGVQLCHNRVGSMFTWFFQAGPVTDWESASKSDTEAFGKFF
                     RGMLDRGVYLPPSQYEAAFLGAAHTEEDVQKTVTAAKEAFAQK"
     gene            complement(3550..3675)
                     /locus_tag="VEJ00_05755"
     CDS             complement(3550..3675)
                     /locus_tag="VEJ00_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00699.1"
                     /translation="MRRVGILEDHIRLRIMHPNQGVESPEELTEDSISLVRAYLK"
BASE COUNT          794 a         1226 c         1025 g          749 t
ORIGIN      
        1 gagagccgcc gtacccgccc ccaccgccgc cgtggaagcc ccagccacca ccaccatgaa
       61 acccgccgcc accaccacca tggcctccgc cgccgcgctg ggcgacagcc gacacgggca
      121 ggcaagcgag cgcaaaggaa gcgattccag caagaagagc gtaccgcaag gtggatttcc
      181 gcatgtcagt actccttcca cccgatggcg gttttgctgc ccgggagggc aaaccgcctc
      241 taccccaatc ttactttatt tggatgtgtt tcgattgtga aagttgccca aactggccgg
      301 ttttacaagc cgtctaggtc gctatagagc cgaggtttgg gcggcgggaa atgacaaaac
      361 ctggggcacc ccggcggcta cgcccccacc gcctcctgcc cgcccagcgg caggaagtat
      421 tgtgtgcctt tgatcggctc gctcaccttg cgcaacagct cccgcaggtc ttcgttgcgg
      481 tcgacgaaat cgatctccga cgtgtttaca atcagcaggt ccgacgatgt gtagtgaaaa
      541 aagaagtgct cgtaagcctt gaccacctct tccagatact catcgctcac cgctcgctcg
      601 cctggcgcgt tcttcttctt cagccgcttc ttgagtaccg ccggtgtcgc ctgcagatag
      661 atcaccaggt caggcgtggg caactgctcg cggaagtaat tgtagtagcg gttgtaggtg
      721 tccagctcct ggtcgctgag gttcaggcag gcaaacagct tgtctttttc gaacagaaaa
      781 tcagcgacga caacccgccg cgaacgcggc cccacatcca gcgcctgcaa tgcttcgaag
      841 cgccgcacca ggaaagcaaa ctgcgtttga aacgccgccc cgcgttcgcc ttcataaaag
      901 gcatcgagaa agggattcga atccggctcc atcacccgct gcgcgttcag ccgttcgccc
      961 agaatctgcg ccagcgtgct cttgccgact cggattgggc cctccactgc gatgtaccgg
     1021 ggcagctcaa agagattggc catgttttcg agaggacgat gtttcggaag ttgcagaata
     1081 gctcggatag agcccagtga caattgaggg aagagttaac agtaatttct cgatttggcg
     1141 atttgagatt gccaattttt cagcgcacca ggtcaatcgg caatcgataa tcgcaaatca
     1201 atttacaatc ctcccatgct cggagccctg ctggctggaa ccaccgcggt cgctgctgtc
     1261 gcggccggct accagtcgat gtcccccacc ggtcagtggt acggccgcac cttcatcggc
     1321 ctggccccgg ggaccaagca actcgctctc acctacgacg acggtccgaa cgatccgcat
     1381 acgctgcgcc tgcttgagct cttggccagg cataacgtgc gggcgacttt ctttctgatc
     1441 ggccgctacg tacagcagtg tcccgaaatc gcgcgtgaga tcgcaaaagc cggacatgtc
     1501 atcggcaacc acaccttcac gcacccgctg ttgactttga acagtaacgc cgagatccgc
     1561 cgccagctcc tagatggcca gtcggcgctg cacgatgcga tcggagaaca ctccaacctc
     1621 ttccgccctc ccttcggcgg acgccgccct gcggtcctgc gcatcgcgcg cgaactgggg
     1681 cttgagcctg tgatgtggaa tgtgaccggt tacgactgga acgccccgcc cgccgccgtg
     1741 atcgagcgga aggtcgcaag gcaaattcgc ggcggcgacg tcatcctgct ccacgacgga
     1801 ggccataaac agatgggtgc cgaccgcagc caaaccgtgc tggcctcgga gcacatgatc
     1861 gcgcgataca aagcaagcgg ctacgagttc gtgacgattc cggagatgat gggaatctcg
     1921 gcttccagac gagggtgatc aactttccgc taggacggtc tgcttctatt tctgagcgaa
     1981 cgcttccttc gccgccgtaa ctgtcttttg cacatcttct tccgtatgcg cagcgcccag
     2041 aaacgccgct tcatattgcg acggcggaag gtacacccct cgatccagca tgccgcgaaa
     2101 gaatttcccg aacgcttccg tgtccgactt cgaagccgac tcccaatccg tcaccggccc
     2161 cgcctggaaa aaccaggtaa acatcgatcc cacgcggttg tggcacagct gcacgccggc
     2221 gtccttcgcc gctgcggcca cgccttccac cagctgtccg ctcagctttt caagccgcgg
     2281 atagatttcg cccttgcgat cacgcagctg tttcagcatc gcgcagcccg ccgccatcgc
     2341 cagcggattt cccgagagcg tacctgcctg gtacatcggc cccagcggag cgaccagatc
     2401 catgatctcg ttcggccccc cgtaggcgcc tacgggcaaa cctcctccga taatcttccc
     2461 aagtgtcgtc aggtccggct tgatgttgta caactcctgc gccccgccaa aagccacgcg
     2521 aaatccggtc atcacctcat caaaaatcag cacggcgcgc tcgcgttccg taatcaggcg
     2581 caaaccaacc agatagtcgt ccgctgccgg aacgcaaccc atgttcccga ccaccggctc
     2641 gacgatcacg caggcaatct gatgtttgaa cttctgaaac gcgtgctcca cggcagaaag
     2701 attgttgaag ggcaaggcca ccgtgaattg gatgaactcc tcgggaacgc ccgcggatcc
     2761 cggaattccc agcgtcgcga ctcctgaacc ggccttgacc agcaaggcgt cggcgtggcc
     2821 gtgatagcag ccttcgaact tcacaatgta tttgcgtttc gtgtaggccc gcgccaggcg
     2881 gatcgccgac atcgttgctt cggtccccga actgacgaag cgtacttttt gcatgtgcgg
     2941 gaaagcagcc agtaccagct ccgcaagatc cgcctccgaa ggcgtcgacg ctccaaagct
     3001 cgtgccgttg caagcggcac ccacaatggc gtcgagcacg ccagcatcgg cgtggcccag
     3061 gatcagcggc ccccacgatc cgatgtagtc gatgtattcg ttctcgtcgg cgtcccagat
     3121 atgggaacct ttgccgcgca ccacgaacag aggatcgccg cccaccgagc gaaacgcccg
     3181 gaccggagaa ttcactccgc cggggatcat gctctccgcg cgttgctgca tgcggcggga
     3241 ctgctcgatc ttgcgcgatg tggtcgaggg catcgaattc tgatgaccgc acaaacttca
     3301 atgtgtcggg gtctgcaacc gaactactat agcagcgccc tgaattaatc agatattcat
     3361 cgttctgaaa tcagtctgca atgttccgaa ggtatcgtaa ccctatcaag ttgcccttct
     3421 aaccgggagg gtttgaggga cagtctcggg ggctccggac tgtccttttt gttttgccca
     3481 tggtgggcgc gggcactcct gcccgcgaat gtcgcaatcg tgacagaact caggagttcc
     3541 ctaggtcact tacttcagat acgcccgcac cagcgagatc gaatcctcgg tcagttcctc
     3601 cggcgactcg accccctggt tcggatgcat gatacgcagc cggatgtgat cctccaggat
     3661 tcccaccctt cgcacaaccc gacattaact acggttaatg ctgaatggta taatcgcgtt
     3721 aactcagctc aagcaaaggc taacgagaat ccggcccgac cggtatcagg ggcaagggaa
     3781 agcaacgcat accg
//
LOCUS       DASPUL010000230         5945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_448621, whole genome shotgun sequence.
ACCESSION   DASPUL010000230 DASPUL010000000
VERSION     DASPUL010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448621"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..2036
                     /locus_tag="VEJ00_05760"
     CDS             129..2036
                     /locus_tag="VEJ00_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00700.1"
                     /translation="MFAAAAPIHAQAPKHPLDGLTAPELWTIYQALQASGKVDEKTRY
                     PMIQLHEPPKEEVLAWKPGQPMRREAFVVVKKGAQTFEAVVDLKGQKLVSWTEVKGVQ
                     PPSPEEEQDDFKDEIKENAEVKAALKKRGITDLGTVECGGYGTGYFGTAEEQGRRLFR
                     VSCGQQFGALENWGPIEGLTILWDANEKKVLRVIDTGVVPVPHAEANYDVASVGKLRD
                     VPTPISVQQPLGPSYRLDGQSVSWQNWNFHFRIDRRVGLIVTNVSYQDGDKLRSILYE
                     GSLSEMFVPYMDPSEGWFARSFFDVGEFGGFSSSLEPGDDCPDNATYFEQMYSDWKGR
                     PHLKPRAACLFEQPSGYMAWRHDGDGIVESRKMRELVLRTIGTFGNYDYVVDWIFQQN
                     GTIRVRLGATGFDEIKAVKARTAAEDADGEANRYGRFIAENTVAVDHDHYFSFRLDFD
                     IDGTANSFVRDKLRVKRLPADSLRKSLWVAEPETAKTENEAKTHMDMAQPENWRIVNP
                     SVKSALGYPVGYELMPGDNAMSLLAPEDYPQKRAGFTDYQVWVTPYRENERYPAGDYP
                     MQSRGGDGLPDWTKANRPIENTDIVLWYTMGFHHVPHAEDWPVLPTVWHEFELKPVNF
                     FARNPALDLPK"
     gene            2110..3156
                     /gene="mqnC"
                     /locus_tag="VEJ00_05765"
     CDS             2110..3156
                     /gene="mqnC"
                     /locus_tag="VEJ00_05765"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HXX00701.1"
                     /translation="MSLTKAQAIEMFESDDLIGIGMEADAVRRRLHPESVVTYIIDRN
                     INYTNFCTEYCTFCAFYRPLKGPAAKEGYILDFDTIYEKIRETVELGGTGVLMQGGLH
                     PDLKIDWHERMLRGIKQRFPQVHLHCYSASEIIAIAEYSGLAIRDTILRLRDAGLDSI
                     PGGGAEILDDEVRYKIARLKCLTDDWINVHRTAHQIGMRTTATMMFGVGEKYEHRVNH
                     FQRLYDLQEETGGFTAFIPWSFQPANTALGGRHWDEATAVEYLKVLAISRLYLSNFLN
                     VQSSWVTQGLKVCQMGLRFGGNDVGSVMLEENVVRAAGVTNCTTEEELRRIIRDAGFR
                     PAQRDTLYRQYFLN"
     gene            complement(3368..3438)
                     /locus_tag="VEJ00_05770"
     tRNA            complement(3368..3438)
                     /locus_tag="VEJ00_05770"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3404..3406),aa:Gly,seq:gcc)
     gene            3554..3766
                     /locus_tag="VEJ00_05775"
     CDS             3554..3766
                     /locus_tag="VEJ00_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00702.1"
                     /translation="MPSYPIKTCVLCGEEFELKPGKPSFANRCPSCSEEVDTKTKHDA
                     DVLKTDKQANAARREAMRNLLYRKDN"
     gene            3962..5662
                     /locus_tag="VEJ00_05780"
     CDS             3962..5662
                     /locus_tag="VEJ00_05780"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXX00703.1"
                     /translation="MKRIATFLLIALPGAFAAAQTPSPAAKPAARAVIEGIVTKDPGS
                     EPVKKALIELIAENQAEGGDYTAVTGADGAFHIDGILAGRYHLFVERTGLLEVDKHRL
                     RTDGRVLNLAAGQEVKDLAIRLQAAAVVRGRVTDEDGDPMPNAQVAVLRQTYVSGRSR
                     WEQAGSERTNDLGEYRIANLAAGSYYVSVSPPPDFKSLIETAGAAALEPRSSAAAGKA
                     DVAYQQTYYPGTVDRGQSAPIQLRAGDDFPVNFSLAPSPSLSIRGSVVNLPPRASPSI
                     MVRSRDFALVQGTDLRPDGSFVLRDVSPGAYTVMATAESGAVAMMARQSVQVTGRSIE
                     GLRLALQPGASIRGRLRLETGGSGGRLDPSRIFLTLHAADGDDDDDGVLPFGVAEASA
                     HLAQVAPDGSFEFTGVPPGNYYVQLEESAGNSDWFLKSAVTAGGEASEAGINVNGGTI
                     SLDLLASTGGVVEGTGSSKDGSPVANAVVVAVPDPHLRGRADRYRKTVSDQAGRFTLR
                     GVPPGEYTLFAWEGVDGEAYLNAEFLKSYEAQGSALRLGAGERKSLQLVVIPEAEEQP
                     "
BASE COUNT         1244 a         1714 c         1877 g         1110 t
ORIGIN      
        1 gcctttcaca ccatcaaccg ggccataagt cttccgaaga aagggaattc gctatggcaa
       61 atgctcgtgt tgtcctctgc aggccagctt tcacgataca aagcattgtg gcagcagtag
      121 ctctggcaat gtttgcagcc gctgcgccga ttcacgcgca ggctccgaaa cacccactcg
      181 acggcctcac cgcccccgag ctctggacta tctatcaggc cttgcaggcc tcgggcaaag
      241 ttgacgaaaa gacgcgctat ccgatgattc agcttcacga gccgccgaag gaagaagtcc
      301 tggcgtggaa gcccgggcag cccatgagga gggaagcgtt tgtcgtggtc aagaagggag
      361 cgcagacgtt cgaggccgtc gtggatttga agggccagaa gctcgtttcc tggaccgaag
      421 tgaagggcgt gcagccgccc tcgccggaag aagagcagga cgacttcaaa gacgagataa
      481 aggaaaacgc ggaggtcaaa gccgctctca agaaacgagg catcaccgat ctggggactg
      541 tggagtgcgg cggttacggg acgggctact tcggcacggc agaggagcaa ggtcgacgct
      601 tgtttcgcgt cagctgtggg cagcagtttg gggcactgga aaattggggg ccgatcgaag
      661 ggctcacgat tctgtgggat gcaaatgaga agaaggtcct ccgcgtcatc gacaccggag
      721 ttgttcctgt accgcacgcg gaagcgaact acgacgtggc atccgtcgga aaactgcgcg
      781 atgttcccac gccgatcagc gtgcagcagc cgctggggcc cagctaccgg ctcgatggtc
      841 agtcggtcag ctggcagaac tggaactttc atttccgcat cgaccggcgc gtcgggttga
      901 tcgtcaccaa cgtgagctat caggatggcg acaagctccg ctccattctg tatgaaggat
      961 ccctctcaga gatgtttgtg ccctatatgg atccgagcga aggctggttt gccaggtcgt
     1021 tttttgacgt cggcgaattt ggcggctttt cgagctcgct cgaacccggc gacgattgtc
     1081 cggacaacgc cacctacttc gagcagatgt actcggactg gaagggaagg ccgcatctga
     1141 agccgcgagc ggcgtgcctt ttcgaacagc cgtcaggcta tatggcctgg aggcatgacg
     1201 gcgacggcat cgtcgaaagc agaaagatgc gcgaactggt gctgcggacg atcggaactt
     1261 tcggcaacta cgactacgtc gtcgactgga tttttcagca gaatggaacc atccgagtgc
     1321 gtctgggagc gaccgggttc gacgaaataa aggcggtcaa ggcccggacc gcagctgaag
     1381 atgccgatgg agaggccaac cggtacggcc ggttcatcgc ggaaaacact gtggccgtcg
     1441 accacgacca ctacttttcc ttccgcctcg acttcgacat cgatggaact gcgaacagct
     1501 tcgtgcgcga caagctgcgt gtgaaacgcc tgccggccga cagcctgcgc aagagtctgt
     1561 gggtagccga gccggagacg gcgaagactg agaacgaagc caagacccac atggacatgg
     1621 cgcaaccgga gaactggcgg atcgtcaatc cgagcgtgaa gagcgccttg gggtatcccg
     1681 tgggatatga gttgatgcct ggagacaacg ccatgtcgct gctggccccg gaagattatc
     1741 cgcaaaagcg cgcgggcttc accgactatc aggtctgggt cactccttac cgggagaatg
     1801 agcgctaccc ggcgggcgat tatcccatgc agagccgggg cggagacggc ttgccggact
     1861 ggaccaaagc caatcgcccg atcgagaaca cggatattgt tttgtggtac accatgggat
     1921 ttcaccacgt gccacacgcg gaagactggc cggtgctccc cacggtatgg catgagttcg
     1981 aactcaagcc ggtgaatttc tttgcccgga atccggcgct ggatctgcct aagtaacggc
     2041 ccaaggccga tggcccggct tggaagcctc gcgcccggtc cgataaaata gacggttctg
     2101 tggatccgta tgtccctgac caaagcccaa gcgatcgaaa tgttcgaatc cgatgacttg
     2161 atcggcatcg gcatggaagc cgacgccgtg cgtcgccgcc tgcatcccga gagcgtggtc
     2221 acctacatca tcgaccgcaa catcaactac accaacttct gcaccgaata ctgcacgttt
     2281 tgcgcctttt accggcccct gaagggcccg gcagcgaaag aaggctacat tctcgacttc
     2341 gacaccatct acgagaagat tcgcgagacc gtcgaactcg ggggcacggg cgtgctcatg
     2401 cagggcgggc tgcatcccga tctgaagatc gactggcacg agcgcatgct gcgcggcatc
     2461 aagcagcgct ttccgcaggt gcatctgcat tgctattcgg cgtcggagat tatcgcgatc
     2521 gccgagtaca gcgggcttgc gatccgcgac acgatcctcc gcctgcgcga tgccgggctc
     2581 gattccatcc ccggcggtgg ggccgagatt ctcgacgacg aagtgcgcta caagatcgcc
     2641 cgcctgaaat gcctgaccga cgactggatc aacgtgcatc gcaccgccca ccagatcggt
     2701 atgcggacca ccgcgaccat gatgttcggg gtgggcgaga aatacgagca ccgcgtcaat
     2761 catttccagc ggctttacga cctgcaggaa gagaccggcg ggttcaccgc ctttatcccg
     2821 tggagttttc agccggcgaa tacggcgctg ggcgggcggc attgggatga ggccacggcg
     2881 gtcgagtact tgaaggtgct ggcgatctcg cggttgtatc tgtcgaattt cttgaacgtg
     2941 cagtcgagtt gggtcacaca gggactgaaa gtctgccaga tgggcctgcg cttcggcggc
     3001 aacgacgtcg gctcggtaat gctcgaggaa aatgtcgtgc gagccgcggg cgtgaccaac
     3061 tgcacgacag aagaagagct gcgcagaatt atccgcgacg ccggcttcag gcccgcgcag
     3121 cgggatacac tgtatcggca gtatttcttg aactagaacg tcaaccgaac cagaatcgca
     3181 aaatacgaac ggccccggaa gcccgaggcc gttcgcgaac agccaataac tggttcctcg
     3241 ccaaggtaac gatttcatta tcgaagcgat gacgcttcaa atcaagttac tttaaggagg
     3301 gtcataccct gccctacgaa ccctccattt gtccctgaga gacaatcctt ccttcagcaa
     3361 gaaggaatgg acctggaggg actcgaaccc tcacctgtac cttggcaagg aaccgtgctg
     3421 ccattacact acaggcccac gccgaagagc ataggctccg gcgagcgcgg actgtggttc
     3481 tgaagtgata ttttcccgca cccagtttcg ttgcgggaaa tgcccaccat gcacacgata
     3541 aaataaaaac agcatgccct cctatccaat caagacctgc gtcctctgcg gcgaagagtt
     3601 tgagctgaag ccgggcaagc ccagctttgc caaccgctgt ccctcatgca gcgaggaggt
     3661 cgatacgaag acgaagcacg acgccgacgt gttgaagacc gacaagcagg ccaacgccgc
     3721 ccgccgggaa gccatgcgca atctgctcta ccgcaaagat aactagcagg cctccattcg
     3781 ccacaaaggg cccgaaacgc acgaaggaaa gccaacaata cgcacgcctt cgtgaaacct
     3841 cgtgtccttg tcggtaaatg aatctcaatc gctggcggca aaccgcccgc ttgcctcgtg
     3901 cgataaaata ttgtttacgc ggccgaccct cacggctgta agcctcccgt tccctggatt
     3961 catgaagaga atcgcgacat tcctgctgat cgccttgccg ggagcatttg ccgccgccca
     4021 gactccgtcg cctgccgcaa aacccgcggc ccgcgcggtg attgaaggca tcgtgaccaa
     4081 ggatccgggc agcgagcccg taaagaaggc gctgatcgag ctgatcgccg agaatcaggc
     4141 ggagggcggg gactatacgg cagtcaccgg ggccgatggc gctttccaca ttgacggcat
     4201 tctggctggg cgatatcact tgttcgttga acgcacgggg ctgctggagg tcgacaaaca
     4261 ccgcctgcga actgatggcc gcgtgctgaa cctggcggcc gggcaggagg tcaaggatct
     4321 agcgattcgt ctgcaggctg cggcggtggt gcgcggccgg gtgaccgacg aagacggcga
     4381 tcccatgccc aacgcccagg tcgcggtgct gcggcagacc tatgtttcgg ggcgaagccg
     4441 ctgggagcag gccggctcgg agcggacgaa cgatctcggc gaatatcgca tcgccaacct
     4501 ggcagcgggg agctactacg tgtcggtgag tccgccgccg gacttcaaga gtctgatcga
     4561 aaccgcgggg gcggcagcgt tggagccgcg tagttcggct gcggcgggca aggcggacgt
     4621 ggcatatcag cagacctact acccaggcac cgtggatcgc gggcagtcag ccccgatcca
     4681 gctgcgtgca ggcgacgatt ttccagtgaa tttctcgctg gcgccgagtc cgagccttag
     4741 catacgcggg tcggtggtca atttgccgcc gcgagcctcg ccttccatca tggtgcgatc
     4801 gcgcgatttc gcgctggtgc agggaacaga tttgcgcccc gacgggagct ttgtgctgcg
     4861 tgatgtctcg ccgggggcgt acacggtcat ggcgacggcg gagagcggag ccgtcgcgat
     4921 gatggctcga cagtcggtgc aggtgacggg gcgcagcatt gagggcctgc gtcttgcact
     4981 gcagccaggc gcatcgatcc gcggccggct caggctggaa accgggggca gtggagggcg
     5041 gctcgatccc agccggattt ttctcacctt gcacgcggcc gatggcgatg acgacgatga
     5101 cggcgtgttg ccattcggcg tggcggaagc atcggcgcac ctggcgcaag tcgccccgga
     5161 tggaagcttc gagttcacgg gcgtcccgcc ggggaactac tacgtgcaac ttgaggagag
     5221 cgccgggaat tccgactggt ttttgaagtc ggcagtcacc gcaggcggcg aggcctcaga
     5281 ggcgggaatc aacgtgaacg ggggaacgat ctcgcttgat cttctggcga gtaccggcgg
     5341 ggttgtcgag ggcacaggtt cctccaagga cggttcaccg gttgcaaatg cggtggtcgt
     5401 tgctgtgcct gatccgcacc tgcgcggtcg cgccgatcgc taccgcaaga cggtcagcga
     5461 tcaggccggt agattcacgc tgcgaggagt tccgccgggc gagtacacgc tgttcgcgtg
     5521 ggagggcgta gacggtgagg cttacctcaa tgccgaattc ctgaaaagct acgaggcgca
     5581 gggaagcgcc ctgcgcctgg gcgcaggtga acggaagagc ctgcagctgg tggtgatccc
     5641 tgaggcggag gaacagccgt agaggctgca tttgaaatcg gatctctttc gcatgcaagc
     5701 gagtgccccg tcctaagcac ggtttttgcg cccagggtgg cgcttttgac tttggtccct
     5761 ttcgttgcag tttaccagcg atgtgtctcg accaccggcg agctcgcgtc cgcggggttg
     5821 gcatcgtcgg cgtggatctt gccgtcatct tcggcgtaaa acgagcggtg ctgcgcatcc
     5881 atgttctttg gcgtcatggt gaggatgtag ctgtctttct ttcctttgta gccgaccgtg
     5941 tagtc
//
LOCUS       DASPUL010000231         4314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_448986, whole genome shotgun sequence.
ACCESSION   DASPUL010000231 DASPUL010000000
VERSION     DASPUL010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4314)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4314
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448986"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..444)
                     /locus_tag="VEJ00_05785"
     CDS             complement(13..444)
                     /locus_tag="VEJ00_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXX00704.1"
                     /translation="MRLTHAEFRDLAWKHGLAATHQRQVVYEAVVASHGHRSPEQIYS
                     AVRKRVPHISLATVYNNLRLFVEHGLLREVTPHSSTLRVDGNLKPHHHLVCSRCKSVQ
                     DIEGDFIDFKRLSPHVPSGFDLTQPVVEVFGLCARCRTKTL"
     gene            complement(655..2364)
                     /locus_tag="VEJ00_05790"
     CDS             complement(655..2364)
                     /locus_tag="VEJ00_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HXX00705.1"
                     /translation="MSQSSPSSSSKPRVRFAPSPTGFLHVGSARTFIFNWLYARRNHG
                     TMILRLDDTDVERNTDASVTSIFEGLKWLDLGWDEEYKQSERLDLHRKMAWSIFEKGL
                     AYRDFTPAHTGDSEKSGPLKIDPKDLRMDTFSSPPSVGVRITHVPTGTVVVSKDEHSE
                     TKNREQALLMLREQLSEGGRGTWLFNPGMRELSRAESDRRAAGEPFALRFRVPREAGQ
                     VVRFTDAVYGEQMKAGADIEDFALLRSDGMPTYHLASCADDADLRISHIIRGQDHLTN
                     TFKHVLIFEAARYTPPQFAHLPLLVAPDGTKLSKRRHGPVVSVTTYRDAGFLPEAFIN
                     FLCLLGWSPKDDRTKLSRQELIDAFSLEGINRANAVVNFKESPLSNNVVIPSGLEPAR
                     NLLSAPDDTFDPKALWLNAEHIRGLAIEGLSAILLPIVQERFPRVTAEKMLQITPLIR
                     ERITLLRDVLTAADFFFVDELPPYDPAELIPQKGDAAMALKALQKAQEVLAQTEFKHD
                     PLDHALRAAAQELGLKAGQMFQPIRVAVCGRKNAPPLFETLEVLGRETTLARIMQATA
                     KVG"
     gene            2641..>4314
                     /locus_tag="VEJ00_05795"
     CDS             2641..>4314
                     /locus_tag="VEJ00_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXX00706.1"
                     /translation="MNSVVITTVVIPGIASVLLFMLFAYLYEQSRQDYFRAWKWAWAF
                     YSLHYVLDAFPASPTAFFSSELFLIAMAMCIFVSTRLMRAPYRFRWYDAVVAAASVLL
                     ALLALRGHIVSGVFRPDAQPVIRLGLGLAAILLYSSAVFYVNGHKRGSLAFQVLAVSL
                     ALWAVLMGVGQLQNPWVEKFGSASRLFGPVPQMLLGIAMVMVLFENQRNAVQENTLAL
                     STLGVDPRRLLFAEDLIPSMQAALERLRSALQMDRAAIVVTERWRGLLPSVQQGFPAG
                     FLESLETSGSGEYICELAYRQSGIFTVQDVAELTEPLPMASVGTFAEFKRVLAEAGVE
                     NLTAVNLQTREHNFGVILFPHAERKAFGSSGPRLMVGLALQLGLTLENYVVTHDAHRR
                     TKEYELLTEIGKAISSRLDQDEILRTIHVELGQIFDNSHFYIAFQEGEEIRFELEVRD
                     NRVQPKRTRELRDAFTEYVIRSGEPLLIRSELEATRKRLGISHVPERPAKCLIAAPIF
                     MGKKAAGVMVAMNPDREFVFEQRDLDVLVTAAGQVSVAMENARLFAEEQR"
BASE COUNT          806 a         1151 c         1448 g          909 t
ORIGIN      
        1 ggtacggcgc ggttataaag tttttgtgcg gcagcgcgcg cagaggccaa agacctcaac
       61 tacgggctgc gtcagatcaa atccactggg gacgtgcggc gacaagcgtt tgaaatcgat
      121 aaaatcacct tcgatgtctt gcacagactt gcagcgtgag cagaccaggt gatgatgcgg
      181 tttgagattg ccgtccacgc gcaaggtgga cgagtgcgga gtgacctcgc gcagaaggcc
      241 gtgctccacg aagagacgga ggttgttata aacggtggcc agagaaatgt gcggaacacg
      301 cttgcgcacg gcggagtaaa tttgctcagg ggaacggtgg ccgtgggaag caacgaccgc
      361 ttcgtagact acctggcgct gatgcgtggc ggcgaggcca tgcttccagg cgaggtcccg
      421 gaattctgcg tgcgtgaggc gcataattat tgaataagaa tgattataaa acagggtccc
      481 gggatgtcaa ccgtccgcag caaccctcgg ttcggtaggg aagctggcgg agggcctcgg
      541 gacagggagg ctctccacaa gtaatgttga cttcgcgttc ccggtttccg ataatcgact
      601 tgtaactgga caggttgaga gccccgaact ggtaacggtt cggggctttg ctttttagcc
      661 tactttggct gtcgcttgca tgatcctagc gagcgtcgtt tctctgccca ggacttcaag
      721 tgtttcgaat aaaggcgggg cgtttttgcg gccgcagacg gcgacgcgga tgggctggaa
      781 catctgtccg gccttgaggc cgagttcctg ggcggcggcg cgcagagcgt ggtcgagcgg
      841 gtcgtgtttg aactccgtct gcgccagcac ttcttgcgcc ttctgcaacg ccttcagcgc
      901 catggccgcg tctccctttt gagggatgag ttcggcggga tcgtagggcg gcagctcatc
      961 gacgaagaaa aaatcggctg cggtgaggac gtcgcgcagc agcgtgatgc gctcgcggat
     1021 gagcggtgtg atctgcagca ttttttctgc ggtgacgcgg ggaaaccttt cctggacgat
     1081 cgggagcagg atcgcgctga gaccttcgat cgctaagccg cggatatgtt ctgcattgag
     1141 ccagagggct ttggggtcga aggtgtcgtc cggtgcggaa agcaggttcc tcgcgggctc
     1201 aagcccgctc ggaatgacaa cattattgga gagtggagac tctttgaagt tcaccactgc
     1261 gttggcgcgg ttgatgcctt cgagcgagaa ggcgtcgatc aactcctgcc gagacagttt
     1321 tgtacggtcg tctttcggag accagcccag caggcacagg aaattgatga aggcttcggg
     1381 caagaatcct gcgtcgcggt aggtggtgac gctcacgacc gggccgtggc ggcgcttgga
     1441 aagcttcgtt ccgtcgggag caaccagaag cggaagatgc gcgaactgcg gaggggtgta
     1501 ccgggcggct tcgaaaatca ggacgtgttt gaacgtgttg gtcaggtggt cctggccgcg
     1561 gatgatgtgg ctgatgcgca ggtcggcatc gtcggcgcag gatgcaaggt ggtaagtcgg
     1621 catgccgtcg gagcgcagca gggcgaagtc ttcgatgtcg gctccagcct tcatctgctc
     1681 gccgtacaca gcgtcggtga agcggaccac ttgtcctgct tcgcgtggca cgcgaaagcg
     1741 aagggcgaac ggttctccgg cggcgcggcg atcgctttct gcgcgggaaa gctcgcgcat
     1801 gccggggttg aatagccagg tgccgcgtcc cccctcgctc agctgctcgc gaagcataag
     1861 caaggcttgt tcgcggttct tcgtctcgga gtgttcgtct ttcgagacca cgactgtgcc
     1921 ggttgggacg tgcgtgattc ggacaccgac cgatggcgga gagctgaacg tatccatgcg
     1981 caggtccttg ggatcgatct taagtggacc cgatttctcg ctatcgcctg tgtgggcagg
     2041 cgtgaagtcg cgatacgcca ggcctttctc gaaaatcgac catgccatct tgcgatgaag
     2101 atcaagcctt tcggactgct tgtactcttc gtcccagccg agatcgagcc acttcagccc
     2161 ttcaaaaatc gaagtgaccg aagcatcggt gttgcgctcg acgtcggtgt catcgaggcg
     2221 aagaatcatg gtgccgtggt ttcggcgggc gtagagccag ttgaagatga acgtgcgggc
     2281 gctgcccacg tgaagaaatc cggttggcga gggcgcgaag cggacgcgcg gcttactcga
     2341 cgatgagggc gaagattgcg acatgcttaa ttcggtggaa agtgagatcg gttcgtcgct
     2401 cgatcaacaa ggctcttccc gcagctacgg cgatcggccg aaatccgatg gtacgggccc
     2461 tccaaatcac cgtcaagcgc aagcgccgta atgacttggc aaagtctccc tggcgccggc
     2521 gccgttgctg cagacaggta cttaaatcct gttgttttcg cgaattcggt gtgaaaacat
     2581 tgagttactg tccccccgga aactgagagc gaattaccca ggggcgcctt ctcggatcca
     2641 atgaacagcg tggtcatcac gacggtcgtg atcccgggca ttgcctcggt gctgctgttc
     2701 atgctctttg cctacctgta cgagcaaagc cggcaggact acttccgcgc gtggaaatgg
     2761 gcctgggcgt tttattcgct gcactacgtg ctggatgcct ttcctgcgtc tccgaccgca
     2821 ttcttctcga gcgagctgtt cctgatcgcg atggcgatgt gcatcttcgt ttcgacgcgg
     2881 ctgatgcgcg caccctatcg cttccgctgg tacgacgccg tggtggcggc agccagcgta
     2941 ctgctggcct tgcttgcgtt gcgcgggcac attgtgagcg gggtgttccg gccggacgcg
     3001 cagccggtga ttcggttggg gctgggactg gcggcgatcc tgctgtattc ctcggcagtt
     3061 ttttatgtca acgggcacaa gcgcgggtcg ctggctttcc aggtgctcgc ggtttcgctg
     3121 gccttgtggg ccgtgctgat gggtgtgggg caattgcaga atccgtgggt ggaaaagttc
     3181 ggcagcgcca gccggttgtt cgggccggtg ccgcagatgt tgctgggcat cgcgatggtg
     3241 atggtgctgt tcgagaacca gcgcaatgcc gtgcaggaga atacgctggc gttgtcgacg
     3301 ctgggcgtcg atccgcggcg gctgctgttt gccgaggacc tgattccgag catgcaggct
     3361 gcgctcgagc ggctgcggag cgcgctgcag atggatcggg ccgccattgt ggtcaccgaa
     3421 cgctggcgcg gccttctgcc ttcggtgcag cagggatttc ctgcgggatt cctggaatcc
     3481 ctggagacga gcggctccgg agagtacatc tgcgaactgg cctaccggca gagcggaatc
     3541 ttcaccgtgc aggatgtggc tgaattgaca gagccgctgc cgatggcgtc ggtgggaacg
     3601 ttcgcagaat tcaagcgcgt cctggccgaa gcaggcgtcg agaacctgac cgcggtgaac
     3661 ctgcagacac gcgagcataa cttcggggtg atcctgtttc ctcacgccga gcgcaaggcc
     3721 ttcggttcat ccggcccgcg gttgatggtg ggcctggcgc tgcaactggg cctcacgctc
     3781 gaaaactatg tcgtcacgca cgatgcgcat cggcgcacca aagaatacga gctgctgacc
     3841 gagatcggga aggcgatcag ttcccggctc gaccaggacg agattctgcg aacgatccac
     3901 gtggagctgg ggcaaatttt cgacaacagc catttctata ttgcgttcca ggaaggcgaa
     3961 gaaatccgct ttgaactgga agtgcgcgac aaccgggtcc agcccaagcg cacgcgggag
     4021 ttgcgggatg ctttcacgga atatgtgatc cgcagcggcg agcccttgct gattcgctcc
     4081 gagctggaag cgacgcgcaa gcggctgggg atcagccatg tcccagagcg ccctgccaag
     4141 tgcttgattg cggccccgat tttcatgggc aagaaggcgg ccggggtcat ggtggcgatg
     4201 aatcccgacc gcgaatttgt tttcgagcag cgcgacctcg atgtgctggt gacggcggcg
     4261 gggcaagtgt cggtagcgat ggagaatgcg cgcctgtttg cagaagagca gcgc
//
LOCUS       DASPUL010000232         3598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_589574, whole genome shotgun sequence.
ACCESSION   DASPUL010000232 DASPUL010000000
VERSION     DASPUL010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589574"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(390..968)
                     /locus_tag="VEJ00_05800"
     CDS             complement(390..968)
                     /locus_tag="VEJ00_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00707.1"
                     /translation="MKRHVLTISLIVLGLCVLGVSASAQDIFKINYFANNGVSGAPDA
                     TVRIDNPGTDSTYNLCAMIYVFDNDQQLTECCGCLVTANGLRTLSVGKNLTNNPLTGV
                     VSNNGVIKIVSSTVNNSPCEPASGVEPTPAVRAWASHIQNKVGSAYPVTETEFSDATL
                     SEQEFSFNLEEQCYFAERLGSGHGICSCGTGD"
     gene            complement(1684..1760)
                     /locus_tag="VEJ00_05805"
     tRNA            complement(1684..1760)
                     /locus_tag="VEJ00_05805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1724..1726),aa:Arg,seq:acg)
     gene            complement(2244..2471)
                     /locus_tag="VEJ00_05810"
     CDS             complement(2244..2471)
                     /locus_tag="VEJ00_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00708.1"
                     /translation="MTYLEVLFNYGATPGENQLRAIDSMREVYGVRRVQFNPKEHTVR
                     VEYDASRLKQDAVAKLLRQAGIDVREPVALA"
     gene            2601..>3598
                     /locus_tag="VEJ00_05815"
     CDS             2601..>3598
                     /locus_tag="VEJ00_05815"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HXX00709.1"
                     /translation="MTASNPAAAPERDPVCGMNVNPATAKHIHEHGGKIYYFCCGSCA
                     EKFKANPAAFLNKTPAPGLVTLGMPAKAASSAGSHAHGGLTSIGATASSPKAAPAAKG
                     APAYVCPMCPEVRETKPGACPSCGMALEAEVPVAATRTEYTCPMHPEVVRAEPGSCPI
                     CGMALEPRTVAAVAEENPELRDMTRRFWVGLVLTVPLLGIAMGNMLWPEVFMRLMGGI
                     AITRGGHVVPLSAIATWVEFLLATPVVLWCGLPFFRRFWASLVNRSPNMFTLIGLGTG
                     VSYVYSVIATVAPQLFPPSLRSMPHYLGVASGSAVPTNTMSGYPDVYFEAAAAITTL"
BASE COUNT          730 a         1099 c         1009 g          760 t
ORIGIN      
        1 cctttaaatt ttgctgcgtc gcgtttattc ccgcgccagc aaaagctgtg aactttggac
       61 tagctgcgtc ctgagcgtat aaagcgggag cattcgtccc cagaatgacc gacaccgcga
      121 atcggatcgc cacacgccgc agtcgaatga tcccgccttt catttcacac tctcaatggc
      181 gcaagaaaca acgacttgtt cagcttgacg cctctctgtg acccgtcaac ctcattgata
      241 gacaagagat taatagggtt tcttttggca ggttgacgca ctctcgcaca aatacgcaaa
      301 cgtcaagttt gagggtgggg ggaagaaaaa agaaggcagc gggcctttat aggagcccgc
      361 cgccttcagg aaccgaggag gtcagttcgt tagtcaccag taccgcagct gcagatgccg
      421 tgtccgctgc ccaggcgctc agcgaagtag cactgctctt ccaggttgaa gctgaattcc
      481 tgttcgctca gagtggcgtc cgagaactcg gtttcagtga ctggatacgc gctgccgacc
      541 ttgttctgaa tgtggctcgc ccaggcgcga actgcgggcg tgggttccac acccgacgct
      601 ggctcgcagg gggagttgtt gacggtcgag gacacaatct tgatgacgcc gttgttggaa
      661 acgacgccgg tcagcggatt gttggtgagg ttcttgccta ccgacagcgt gcgcagcccg
      721 ttggctgtaa ccaggcagcc gcagcactcg gtcagttgct gatcgttgtc aaacacataa
      781 atcatcgcgc acaggttgta cgtgctgtct gtgcccggat tgtcgatgcg gactgtggcg
      841 tccggagcac ccgacacgcc gttgttcgcg aagtagttga tcttgaagat atcctgagct
      901 gacgcgctga ctcccagtac gcaaagcccc aggacgatca gggaaatcgt tagtacgtgg
      961 cgcttcatct aacacctcct attgtagtgg acctgctgtg ctggagggag ggagcagccc
     1021 gtagcacggc cccagcaaac ggtactccat ccacgattga gtgcaagtac ccgtgttaac
     1081 tagcaccgat cttctaggcg ttcaagctaa tccaaatggc taagccagaa atggggcagg
     1141 cgggcaggct tcaagatgac cctttccagt accacgaatt gactcgttcg cccggaagct
     1201 tcttgctttc ggtgccggcc ttccttcgca cctgtccggc gttcagcttg tcgaggtcat
     1261 agccctccgc cttgagcagt tctgagtatc ccctagccgg tccgcttggc cccctgcagc
     1321 aacttgcgaa gaatcttggc ggcctcgtct ttcttgtgct ttgtctttga gagaagtagg
     1381 gtcaacactg tcttcggttg tggcttcaga cttgccagtc caaactacgt gaatgatcgc
     1441 agtggaggag ctgtcgggac tttgatccgc tacaagttcc gtgcgctacc ggaaggccga
     1501 gccctccccg atcgaaaagc gccttttccc ggttcgaggc ctacaacgcc acgaaagagg
     1561 cggaactgga cagagcggaa cggaggatgg tgcagtgagg agtccctaca cttttcacta
     1621 cagtgaccac gcggttatcc cacctgacga gcaccaaaca tcccgtaagt tgttgggaaa
     1681 gaatggtgcg ccctgagaga ttcgaactcc cgaccttctg gttcgtagcc agacgctcta
     1741 tccaactgag ctaagggcgc ggatcggatt ggcaggaaga agcaggaccg attatagcgg
     1801 aaaacatcgc aggccgaaaa cggcggcggc gtgtgcctgc gaaaatccgc cagagctgca
     1861 cgcgacctag ggcaaaaccc ttgcccgtcg cggcttttca acagcttctg gccctgtccg
     1921 tgcctcgcgc cgcctgaagt aaatctttct caggggcggg caaatcctac aatgtcgaaa
     1981 aacccttcgg cctttatttt gaacagcttg tctacggcac gccagatgct ctaaccagac
     2041 cagacgaagt ggcgcgcaac ggggcgctcc agagaataac ttcggccaga ggttttcttg
     2101 agtgcccctt gctttagttt ctagtttcgc agctctcccc gctcccctcg tcatcagccg
     2161 gcctgagtct cccccgactc cggcctttct gttttttgag ggattacttt ccgacaacct
     2221 ggccgcgtac gaatccctga cagtcaggct agggcgaccg gctcgcgcac gtcgataccg
     2281 gcctggcgca gcagcttcgc cacggcgtcc tgtttgagcc gcgaggcgtc gtactcgacc
     2341 cgaaccgtat gctccttcgg gttgaactgt acccgccgca cgccgtagac ctcccgcatg
     2401 ctgtcgatag cccgcagctg gttctcgccg ggagtcgcgc cgtagttgaa caaaacctca
     2461 agataagtca tggtcagacc ttcgatatga tgagttcgtg gaagaaccgg ccggctgccg
     2521 gcttgaatct actatagacc aatgccaatc aaacagggca agcgccgggc agaacccggc
     2581 tctgccttga ccgcgagttc atgacagcct cgaacccagc tgcggctccg gaacgcgatc
     2641 cggtctgcgg gatgaacgtc aatcccgcaa ccgcgaaaca catccatgaa cacggcggga
     2701 agatctatta tttttgctgc ggcagctgcg ccgagaagtt taaagccaat ccggcagctt
     2761 tcctgaacaa aaccccggcc cccggcctgg taacgcttgg catgcccgcg aaggccgcct
     2821 cgtctgccgg aagccacgca cacggcggcc tgacgagcat aggggcaaca gcttcgtccc
     2881 caaaggccgc cccagcagcc aagggtgcgc cggcctacgt ctgccccatg tgccctgagg
     2941 tgcgcgaaac gaagcccgga gcctgccctt cctgcggcat ggcgctggaa gccgaagtcc
     3001 cggtggccgc cacccgcacg gaatacacct gccccatgca tcccgaggta gttcgtgccg
     3061 aacctggctc gtgccccatc tgcggtatgg ccctcgaacc gcgcacggtc gccgcggtcg
     3121 cggaagaaaa tcctgaactg cgggatatga ctcggcgctt ctgggtcggc ctcgtattaa
     3181 ctgtaccgct gctcgggatc gcgatgggaa acatgttgtg gccggaagtt ttcatgcgcc
     3241 tcatgggcgg aatcgcgatc actcgaggcg gtcacgttgt ccctctcagc gcgatcgcca
     3301 catgggtcga attcctgtta gcaacccccg tcgttctctg gtgcggcctg ccgttcttcc
     3361 ggcgcttctg ggcgtcgctc gtcaatcgca gccccaacat gttcaccctg attggcctcg
     3421 gcactggcgt gtcgtatgtc tatagcgtaa ttgccacggt cgcaccgcag ctcttccctc
     3481 catcgctccg atccatgccc cactatctag gagttgcgtc aggatccgcg gttccaacga
     3541 atacgatgag cggctatccc gacgtttact tcgaagcggc agccgcgatc acgacgct
//
LOCUS       DASPUL010000233         6941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_999652, whole genome shotgun sequence.
ACCESSION   DASPUL010000233 DASPUL010000000
VERSION     DASPUL010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999652"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VEJ00_05820"
     CDS             complement(<1..233)
                     /locus_tag="VEJ00_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00710.1"
                     /translation="MADLVSHPDEVKVVSRRFGMGATDIDRYLALDGYKAVQKALTME
                     PDAIINEVKNSALRGRGGAGFSAGMKWSFVPKQS"
     gene            complement(328..810)
                     /locus_tag="VEJ00_05825"
     CDS             complement(328..810)
                     /locus_tag="VEJ00_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXX00711.1"
                     /translation="MRFSEEFEARFAEMVPHYPTKRSALVPTLLYAQDEVGFLSDEVI
                     AEIASRLDLTELEVRNVISYYSMLTTKPRGKFNVQVCTNISCMVRGGEEILRHCAKKL
                     GIGHKGTTPDGLFSLEEVECIGACSWAPAAQVNYDFHENLTTEKMDRVLDDYRKKGTQ
                     "
     gene            complement(858..1091)
                     /locus_tag="VEJ00_05830"
     CDS             complement(858..1091)
                     /locus_tag="VEJ00_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00712.1"
                     /translation="MSTQPEQLRDRTKAFALRVIRLYRSLPYRTDAQAVLWLELMTES
                     GVVPLAKAAALLKEANELTAIFAASQRTARHIA"
     gene            complement(1155..2360)
                     /gene="nuoD"
                     /locus_tag="VEJ00_05835"
     CDS             complement(1155..2360)
                     /gene="nuoD"
                     /locus_tag="VEJ00_05835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HXX00713.1"
                     /translation="MAHLTPTPVVEPGQDRTMILNMGPQHPSTHGVLRVLLEIDGETV
                     VRLMPDIGFLHTGIEKTCEAKFYQQVVPLTDRIDYLCPMTNNLCYVLAVEKLLGLEIP
                     PKAQWLRVLLNELTRINSHLVWLGTHALDIGAMTVFLYCFREREEVLRMFEMVSGQRM
                     MTSYFRVGGIALEPPLGFFDRVRDFAGYFPEKVDEYENLLTGNPIWGMRTKGVAVISA
                     EDAIALGATGPTLRGSGVDIDLRRDMPYSSYEKFQFKVPISQDGDVFARYMCRVQELR
                     ESIGIVRQALDGMPEGPIKADAPHVVLPDREKMKTQMESLIYHFKIITEGFAVPAGEV
                     YQAVESPRGEMGYYIVSDGTAKPYRVHMRSACFANLQTLATMCEGRLIADVVAAIGSI
                     DIVLGEIDR"
     gene            complement(2381..2878)
                     /locus_tag="VEJ00_05840"
     CDS             complement(2381..2878)
                     /locus_tag="VEJ00_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXX00714.1"
                     /translation="MPLAPAITDLEQLKSHPAVARLVAWNAAAVQGVKFDREEMTISV
                     DRANLREACSLLKDDPACPFNFLSDITCVDWYPAEPRFEVVYHLLSIPKKERVRLKVR
                     LNSASPAVESLTPVWAGANYFEREVFDLFGIRFTGHPYLRRILMPEDWEGHPLRKDYP
                     VEGYR"
     gene            complement(2878..3288)
                     /locus_tag="VEJ00_05845"
     CDS             complement(2878..3288)
                     /locus_tag="VEJ00_05845"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXX00715.1"
                     /translation="MPDNYFARYLPFLIHILMAGGIAVAIVVLSWLIGQRKPTRAKLS
                     PYECGMTPVGDSRERFSVKFYLVAMLFILFDVEAVFIYPWAVILRQLKAQNQGMFGLW
                     EMLVYIGIFLVGFFYVWKKGVLDWGETGAKRGKV"
     gene            3480..4061
                     /locus_tag="VEJ00_05850"
     CDS             3480..4061
                     /locus_tag="VEJ00_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00716.1"
                     /translation="MIFGFNTDVKHGDTIYHVQSEARENERLLQTQVFVRGRCIGKKA
                     TSYAGKSSDAQFGDTQKEQQLREQHRLVLDAIREGKLDQALDRPEPEVLATVKELDVL
                     WLNADSIHSDRNLNLQLRVTEGGTAAPGARLTFRFARPGAAPFYTQAVTDSGGAAEIK
                     IEVEESALPDASLLVQANFEGRTATRKFSLRKS"
     gene            4113..4343
                     /gene="thiS"
                     /locus_tag="VEJ00_05855"
     CDS             4113..4343
                     /gene="thiS"
                     /locus_tag="VEJ00_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HXX00717.1"
                     /translation="MKLHLNGEEHDFTSSAAPFTLAALIETLGMKADRVAVELNRDIV
                     PRDRWAATELKEGDRLEVVHFVGGGSERQELS"
     gene            4446..4919
                     /locus_tag="VEJ00_05860"
     CDS             4446..4919
                     /locus_tag="VEJ00_05860"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXX00718.1"
                     /translation="MQDRFTSREVIALTGITPRQLQWWDERGVVKPDRDGHRRLYSVN
                     QLTELAVICELRRKGFSLQGVRKVMRFLAREFGKGLAEIVSRDSEYHLLTDGTHLFLE
                     TSARQIVDILKNSSQPILAVCLSDAVRQVRAGVVSGKANTSVTSPHQRRRARKAS"
     gene            4972..5235
                     /locus_tag="VEJ00_05865"
     CDS             4972..5235
                     /locus_tag="VEJ00_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00719.1"
                     /translation="MVAELELLNEWIPEQMQPGTVFVLENAGEIGEKEDPYWAVLSCP
                     SCGMLGLITRKQINGLLPVICGSGQCSAQFFIRDAEVVVRKPF"
     gene            complement(5311..6294)
                     /locus_tag="VEJ00_05870"
     CDS             complement(5311..6294)
                     /locus_tag="VEJ00_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXX00720.1"
                     /translation="MVTHVASIETTGHKRTLPAGMIDDARKHVYEAAIRTPLVRLNYD
                     GPAEIYLKLECLQPIGAFKIRGAHNAVRLLPEEQRRRGVWTISAGNAAQGVALAARKA
                     GVACKVLVIDVAPEAKLQAIRRLGAELVKGSYDECWKALADRAHPAMNGAFVHPFEDD
                     EFIAGNATAGLEILEDLPDVEAIVASFGGGGLSCGIASAMKERAPHVKVFAAEPQTAA
                     PLAVSLAAGSPQTFANWKASWVDGCGGKSVFPRMWALAHHLLAGSIVCTLEEVRQAMR
                     IVAERNHVVCEGAGACAVAAGLSGKCGSGKVVCVVSGGNIDLSVFSQLVRD"
     gene            complement(6513..>6941)
                     /locus_tag="VEJ00_05875"
     CDS             complement(6513..>6941)
                     /locus_tag="VEJ00_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00721.1"
                     /translation="DIEHSESPVLVRMHAHCLMGDVFGATGCECHATLEGALRRISQE
                     GCGALIYLHQTSQGFSVEKVGERNALSFHRGRKLPSLYETERQIQREIGIGAQILSDL
                     DLRRIRLLTNRPKKVAALEGFGIEIVEQVPVELGEIEKKA"
BASE COUNT         1441 a         2127 c         1978 g         1395 t
ORIGIN      
        1 gactgcttgg gcacgaacga ccacttcatg ccggcggaaa atcccgcacc gccgcgcccg
       61 cgcaaggccg aattcttaac ctcgttgata atggcgtcgg gttccatggt gagcgctttc
      121 tgcacggcct tgtagccgtc cagcgcgagg tagcggtcaa tgtcggtcgc gcccatgccg
      181 aaccgccggg agaccacctt gacctcgtcg ggatgggaaa ccaaatcagc cattgcgatt
      241 gatgatttga agatttcaga ttgcagattt tgaggcccta gcgaaagccg tttttaatct
      301 gcaatctgaa atccccaaat ctgaaatcta ctgcgttccc ttcttcctgt agtcgtcgag
      361 aaccctgtcc atcttttcag tggtcagatt ctcatgaaaa tcgtagttaa cctgagcggc
      421 cggggcccag ctgcatgctc caatgcactc cacttcttcg agagagaaca aaccgtcggg
      481 agtcgtgccc ttgtgaccga tgcccagctt tttcgcacaa tgccgcagaa tctcctcgcc
      541 tccgcgcacc atgcagctga tgttggtgca cacctgcaca ttgaatttgc cccgaggctt
      601 cgtggtcagc atggagtagt agctgatcac gttgcgcact tcgagttcag tcaagtcgag
      661 gcgggaggcg atctcggcga taacttcatc cgagaggaag ccgacttcgt cctgggcgta
      721 gagcagcgtc ggaacgagcg ccgaacgttt ggtcggatag tgcgggacca tctcggcgaa
      781 acgcgcttcg aattcttcgg aaaaacgcat aagtcaattc agcgattgag tcatcgggcg
      841 attgcgcgag tgaaaactca cgctatgtgc ctcgccgtac gttgcgaagc cgcaaaaatc
      901 gctgtcagct cgttcgcttc ttttagtagc gccgctgcct tagccagcgg aacgactccg
      961 gactcggtca tcagttccag ccacagaacc gcctgcgcgt ctgtgcggta cggcagcgac
     1021 cggtacagcc gtatcactcg caacgcgaat gctttcgtcc gatcgcgcaa ctgctcgggc
     1081 tgggtactca taaccgcctt cctcaactcc gcaaaccaat cgcccaatga cccgctcact
     1141 caatgactaa attcctaccg gtcgatctct cccagcacaa tatcaatgct cccgatcgcc
     1201 gccacgacat cggcgatcag ccgcccttcg cacattgttg ccagcgtctg cagattggca
     1261 aagcacgccg agcgcatgtg cacgcggtaa ggtttcgcgg tcccgtcgct gacgatgtag
     1321 tagcccatct cgccgcgcgg cgattcgacg gcctgataaa cctcgcccgc cggcaccgca
     1381 aatccttcgg tgatgatctt gaagtgatag atgagcgact ccatctgcgt cttcatcttt
     1441 tcgcggtccg gcagcacgac gtgaggagca tccgctttaa tcggcccctc gggcataccg
     1501 tcgagcgcct gccgcacgat gccaatcgat tcgcgcagtt cctgcacgcg gcacatgtat
     1561 cgcgcgaaga catcgccgtc ctgcgaaatc ggcactttga actgaaactt ctcgtaactc
     1621 gaatacggca tgtcgcgccg cagatcgata tcgacgccgc ttccccgtag agtcgggccg
     1681 gtcgcgccca gcgcaatcgc atcctcagcc gagatcacag ccacgccctt ggtgcgcatg
     1741 ccccagatgg ggttgccggt cagcagattt tcgtactcat cgaccttttc cggaaaatat
     1801 cctgcaaaat cgcgcacgcg atcgaaaaat cccagcggcg gttccagcgc aatgccaccc
     1861 acgcggaaat acgaggtcat catgcgctgc ccgctgacca tttcgaacat gcgcaggacc
     1921 tcttcacgct cgcggaagca atacaggaag accgtcattg cgccgatgtc gagcgcgtgc
     1981 gtgcccagcc agaccaggtg cgagttgatc cgagttaact cgttcagcaa aacccgtagc
     2041 cactgcgcct ttggagggat ctccaacccc aacagtttct cgaccgccaa cacgtagcag
     2101 agattgttgg tcatggggca gaggtagtcg atgcggtcgg tcaacggcac gacctgctga
     2161 tagaacttgg cttcgcaagt cttctcgatt ccagtgtgca ggaagccgat gtcgggcatc
     2221 agccgcacga cggtttcgcc gtcaatctcc agcagaacgc gcaacacgcc gtgagtcgag
     2281 ggatgttgcg gacccatgtt caggatcatg gtgcgatcct ggccgggttc gacgacgggg
     2341 gtgggcgtca ggtgtgccat cgtccttggt gataaagcgg ctagcggtag ccctccacgg
     2401 gatagtcttt gcgcaggggg tggccttccc agtcttccgg cattaagatc cggcgcaaat
     2461 aagggtgccc ggtgaagcgg atgccgaaca gatcgaaaac ttcgcgctcg aagtagttcg
     2521 cacccgccca caccggggtc agcgattcga cggcggggct cgcactgttg agtcgcactt
     2581 tcagtcgaac ccgctctttt ttcgggatcg acagcaggtg gtaaacaact tcaaagcgcg
     2641 gctcggccgg ataccagtct acgcaggtga tatcggagag gaagttgaag gggcaagcag
     2701 gatcgtcctt taagagactg caggcctcgc gaaggttggc ccgatccacg gagatggtca
     2761 tctcttcgcg atcaaacttc acgccctgca cagccgccgc attccatgcc accaggcgcg
     2821 ccaccgcggg atgactcttc aactgctcca gatcggtaat cgcaggggcc agcggcatca
     2881 gacttttccc cgcttcgcgc cggtctctcc ccagtccaga acgccctttt tccagacgta
     2941 gaagaagccg accaggaaaa tcccgatata caccagcatc tcccatagtc cgaacatgcc
     3001 ttggttttgt gccttcaact ggcgcaggat taccgcccac gggtagatga aaaccgcttc
     3061 tacgtcgaac agaatgaaca gcatggcgac caggtagaac ttcaccgaga agcgctcgcg
     3121 cgagtcgccg accggggtca tgccgcactc atagggagaa agcttggcgc gggtgggttt
     3181 acgctgtccg atcagccagg aaagcacgac aatcgcgacc gcaatgccgc ccgccatcag
     3241 gatgtgaatg aggaacggga ggtagcgtgc gaagtaattg tcgggcatag gaggagatat
     3301 atataattcg cgccaagcac tgcttaaacg ttctagatta tgggcttgca cgtgagagtg
     3361 tcaagcgagg gggaaggaca ttgagtgatt gtgtaatttg gtaaccgagc aattgaaacc
     3421 cgctggtttt agagtttttc agttgcccga ttagacaatt tctcaactac gtaatctcta
     3481 tgatttttgg cttcaatacc gacgtaaaac acggcgacac gatctaccac gtgcagagcg
     3541 aggcgcggga gaacgagcgt ctgttgcaga cgcaggtctt tgtccgcgga cgctgcatcg
     3601 gcaagaaggc cacctcgtat gccggcaagt cctccgatgc gcaattcgga gacacgcaaa
     3661 aagagcagca actgcgcgag cagcatcgcc tggtgctgga tgcgattcgc gaagggaagc
     3721 tcgatcaggc cctcgaccgg cccgaaccgg aagtgctggc cacggtcaaa gagcttgacg
     3781 tcctgtggct gaacgctgac tccattcatt ccgaccgcaa tctgaatctg cagctgcgcg
     3841 tgaccgaggg cggcaccgca gcgccgggcg cgcgattgac gttccgcttt gcccgtcccg
     3901 gtgcagcgcc cttttacacc caggctgtaa ccgattccgg aggagccgcc gagatcaaga
     3961 tcgaagtcga ggaatccgcc ctgcctgacg cctccctgct ggtgcaggcc aattttgaag
     4021 ggcgaaccgc gacccggaaa ttcagcctcc gcaaaagctg actaagcgga gcgctacaat
     4081 caggtcatgg agcggtatcg tcccgcccgc caatgaaact ccacctcaac ggggaagagc
     4141 acgacttcac cagttctgcc gcgccattca ctctcgcggc gttaatcgag acgctcggca
     4201 tgaaggcgga tcgagtggcc gtcgaactga accgcgacat cgtgcctcgc gatcgctggg
     4261 ctgcgaccga gcttaaggaa ggcgaccggc tggaagtcgt tcattttgtt ggcggcggca
     4321 gcgagcggca ggaactctct tagtcacccc ttttcctcag aatctccgct tgacccctgc
     4381 catctcatgc ctaaaatgtt tcaaacattt ggcttgaagg ctgtttcaaa catttctggg
     4441 gatggatgca agatcgcttt acatcgcgcg aagtgatcgc attgacgggc atcacgcccc
     4501 gccagttgca gtggtgggac gaacgcggcg tagtgaagcc ggaccgcgat ggccatcgcc
     4561 ggttgtactc ggtgaaccag ttgacggaac tggccgtgat ctgcgaattg cggcgcaagg
     4621 ggttttcgct gcagggtgtg cggaaggtta tgcgtttctt ggctcgtgaa ttcgggaagg
     4681 ggttggcgga gatcgtcagc cgcgactccg aataccacct gttaacggac ggcactcatc
     4741 ttttcctgga aacatcggcg cgccagatcg tcgatatcct caagaattcg agccagccga
     4801 ttctggccgt ctgtctgagc gatgcggtgc gacaggtgcg ggccggggtc gtctctggaa
     4861 aggcgaatac ttcggtaacc tcgccccatc aacggcggcg tgctagaaag gcctcataga
     4921 ccagttacag cgcagcaggg cagagaagca cgagaggcac agggaggaag catggtggcg
     4981 gaactggagc tcctgaacga gtggatcccg gaacagatgc agcctggcac cgtctttgtg
     5041 ctggaaaacg ccggagaaat cggcgagaag gaagatccct actgggccgt gctgtcatgc
     5101 ccgtcctgcg gcatgctcgg cctgatcacg cgtaaacaga tcaacggctt gctcccggta
     5161 atttgcggct cgggccagtg ctcggcgcag ttctttattc gtgacgcaga agtcgtggtg
     5221 cgcaaaccgt tctagtgtgg cgcgggcact cctgcccgcg aaatgccgac gccaaattga
     5281 actcgatggg aacctgagtt gctggcgtat ctagtcacgc acaagctgtg agaacaccga
     5341 caaatcaata ttccccccac tcaccacgca cacaactttt cccgatccgc actttccgct
     5401 gagccccgcg gccacggcgc aggcaccggc gccctcgcag accacgtgat tccgctcggc
     5461 cacgatgcgc atcgcttgcc gcacttcttc cagcgtgcaa acgatcgacc cggccagcag
     5521 atgatgtgcc agggcccaca tcctggggaa cacggatttg ccgccgcagc cgtccaccca
     5581 gctcgccttc caatttgcaa atgtctgcgg cgaacccgcc gccagcgaga ccgccagcgg
     5641 agcggcagtt tgcggctcgg ccgcgaacac tttaacgtgc ggcgcgcgct ctttcatggc
     5701 cgatgcgatt ccgcacgaca gccctccccc accgaacgaa gcgacgatgg cctcgacatc
     5761 cggcagatcc tcaagaatct ccagtccagc cgtggcgttg cctgcgatga attcgtcgtc
     5821 ctcgaaggga tgcacgaagg cgccgttcat cgccggatgt gcccggtccg ccagagcctt
     5881 ccagcattcg tcgtaggatc ctttcaccag ttctgcgccc agccggcgta tcgcctgaag
     5941 cttagcctcg ggagcgacat caatgacgag caccttgcac gccacacccg ccttgcgcgc
     6001 cgccagcgcc acaccctgcg cggcattgcc cgcgctgatg gtccacaccc cgcgccggcg
     6061 ctgttcttcc ggcagcaggc gaacggcgtt gtgtgcgccg cgaatcttga aggcgccaat
     6121 gggttgcaag cactccagct tgaggtagat ttccgccgga ccgtcgtagt tgagccgtac
     6181 cagcggcgtg cggatggccg cctcgtacac gtgtttgcgg gcgtcatcga tcatgcccgc
     6241 tgggagggtg cgtttgtgcc cagtcgtctc gatcgatgcg acatgagtga ccatagtgat
     6301 tcgccaaggc cccgtgtttc tgttttgaaa acccttgtga tttcgctttg aaagggcgcg
     6361 ccttcaaggc gcgccgttga gagtcgctaa gatcgttggc ctttaggccc cgaggtccgc
     6421 gccctcagcg gctaaagccg cattccttcg cctcatttgc ggcacggctg gaagccgtgc
     6481 cctttcaaaa cgaactcgct caggcagcgt atctacgcct ttttctcgat ctcacctaac
     6541 tccactggca cctgctcgac gatctcgatc ccgaatcctt ccagcgccgc tactttcttg
     6601 gggcggttgg tgagcaggcg gatgcgccgc agatccagat ccgacagaat ctgcgcgccg
     6661 atcccaattt ccctctgaat ctgacgctca gtttcgtaca gcgacgggag ctttcggccc
     6721 cggtggaagc tgagcgcgtt gcgctcgccc actttctcga ccgagaagcc ctgcgaagtc
     6781 tggtgcaggt aaatcaacgc gccgcagcct tcctgggaaa tccggcgcag cgcgccttca
     6841 agcgtggcgt ggcactcgca gccggtcgcg ccgaaaacgt cgcccatcag gcaatgcgca
     6901 tgcatccgca ccagcaccgg agactcgctg tgctcaatgt c
//
LOCUS       DASPUL010000234        11352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1270213, whole genome shotgun sequence.
ACCESSION   DASPUL010000234 DASPUL010000000
VERSION     DASPUL010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 11352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11352)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11352
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1270213"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..725)
                     /locus_tag="VEJ00_05880"
     CDS             complement(51..725)
                     /locus_tag="VEJ00_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXX00722.1"
                     /translation="MLKLILADNQAIFRAGIAKVLAVEDEMRIVAQAQTPEQMFMALD
                     KFRAAVLVVAAGFHNDFAAIVQAANKAKTRVIVLADTGESAQRHMSNGAHGVVYRNVT
                     SAALVDCVRKVARGETWVQDVAVPSELTENDMVGLRVRDRLTAKELRIVALIVQGYKN
                     KEIATQLGTTEQVIKNYLRNVYDKIGVSDRLELALFTIHHRILNEAAAATASGTVPQP
                     PAGVAQ"
     gene            complement(773..1147)
                     /locus_tag="VEJ00_05885"
     CDS             complement(773..1147)
                     /locus_tag="VEJ00_05885"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXX00723.1"
                     /translation="MTLALADDRLNTRRLGGGTVGEEARTGKRFPLELPIKIHKGETD
                     AKGVTGNLSAAGVYIRADATMEIGSPVEFEITLPPEMTGGQENVTIQCKGRVVRADET
                     GTGGEGRGVACVIDTYEFVRNS"
     gene            complement(1289..3085)
                     /locus_tag="VEJ00_05890"
     CDS             complement(1289..3085)
                     /locus_tag="VEJ00_05890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXX00724.1"
                     /translation="MHSHAFAYSPARSSTTRTSALAEGSCYNHTLPMIPPTVQLAESK
                     PQHRSEKVCWITLLAATAASCLFQIAWFWRFRAHNITMDGIAYIGLARHLVDGDVKAS
                     LHGYWSPLTSWIIAASALFGRNFTLLGRLVTITSFLLCPPLLYLLTLKLWRSRVAAAL
                     SVLWFSTARGVAATAVGLILADFVLTACVLLYFVLLLRVLRQDRPVGWLAVGAAHALT
                     FLAKAIAMPWLAISTVLALLVQNARSPRRLTASLLLVFLFPAAVWIGWGLALRSKYGQ
                     FTTGDQLRTNLMINWKRRLSHRPRGDNLAFVDTSSVYDHYMVGESPLAAVQGFSLRRP
                     GMLSMILGSEFQNLPQALKETGILLNPGGVLALAAMLAALAKKRSQYRTQAAFVSIAL
                     LSVLSVIVAYCMLVFDARYVIPVLPLLIAISCPALLPAEFAPDAPGVPGWQRKTAAGL
                     LIVGTVFFTVYWASPFRTVDRDFEASCYQAAAILRAANPHGTLASIGDGPYPQDGVGF
                     EAAPYVAYLAEWRLVAANAELPGLAAADTLAAKALATKADALAVWGSSASPVYRHIVE
                     TIEHASASSAARTITDPYLGEVGTLFLRPRTD"
     gene            3122..4624
                     /locus_tag="VEJ00_05895"
     CDS             3122..4624
                     /locus_tag="VEJ00_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXX00725.1"
                     /translation="MTTSFPRRFPANLRLILASCLIVLISRTSGLSQTGGAKPAPTTQ
                     KKGGGATETKASEAPSVLVQLNGALEGLAAKVSPAVVQILVTGYGPLKEEDRSQTALI
                     VRQHAVGSGVIVDPNGYIITNAHVVEGAQRIRVALPLPMGDSSGQVPIGKRRILEARL
                     LGQHKETDLALLKIEETDLPTLALLTQQRPHVGQLVFAIGSPEGLQNSVTMGVVSAVA
                     RQVDPEKPLTYIQTDAPINPGNSGGPLVDMNGAVLGINTFILSQGGGSEGLGFAIPAR
                     IVEFVYHSLRAYGHVHRVEIGAAAQEITPTLADALHLPRHWGVIVDDVRPGGPAAAAG
                     LQIQDIILTADDRRIETLPSLSAALYLHRIDQVIKLEILRGEERKTLYVPAIEHHDQM
                     DQLLDAVNPANSLIPRLGVLGIDLTPDLASHLGSLRIASGVIIVGRAADLIIPDTGLQ
                     AGYIIHRFNGAPIDSMEALRSAVAALKSGDPVAMQVERDGGLTYVSFEVE"
     gene            4776..5642
                     /locus_tag="VEJ00_05900"
     CDS             4776..5642
                     /locus_tag="VEJ00_05900"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HXX00726.1"
                     /translation="MSENRQTLEFDVEKYIRNSKKVDISDLDLAQASRYPVSEDEVRA
                     LTYMCDIESHTIVYLRAILNTCAVEDPQTTSFLSCWAYEEFFHGQTIRQFLDAIGAPV
                     SSTRMLEVQKATSFHEWLKELGSSILCRFSRHFHGAYLTYGAISELSTLEGYGVMARR
                     TQNPILAEIVRRLAKDERRHFSFYYNKARLELQAKNAQRLTNFVIRNFWLPVGGGVKP
                     DSEVSWILSFILGDPEGAEIASRIDTAIAKLPGLDWFDRLSRSRAEALQETGMQPAQE
                     IRTAVRFFAEPI"
     gene            complement(5659..6042)
                     /locus_tag="VEJ00_05905"
                     /pseudo
     CDS             complement(5659..6042)
                     /locus_tag="VEJ00_05905"
                     /inference="COORDINATES: protein motif:HMM:NF013033.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004800 - thyroxine 5'-deiodinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deiodinase-like protein"
     gene            complement(6055..6357)
                     /locus_tag="VEJ00_05910"
     CDS             complement(6055..6357)
                     /locus_tag="VEJ00_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00727.1"
                     /translation="MAATFSVVAVLWLLGCGAIYHAMRQPPESFGRFMSRIPAPVAFL
                     VFPFETLWTRARTGKLAVGDSAPDFKLTKLDKSAQVQLSSLTAQGKPVALIFGSYT"
     gene            complement(6523..7074)
                     /locus_tag="VEJ00_05915"
     CDS             complement(6523..7074)
                     /locus_tag="VEJ00_05915"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXX00728.1"
                     /translation="MTFIIVILLILIVAAVVLIGMYNGLVQRRVSCDSAWSDIDVQLK
                     RRHDLIPNLVETVKGYAAHEKGTFENVAKFRSQAMQAATPADKAAAENLLTGALKSLF
                     AVAESYPELKASEEFMQLQGSLSQTENSIQNARSNYNAAVRDLNTQIQSFPASILAGM
                     FSFTPRQFFETTAADREPVQVKF"
     gene            complement(7169..8209)
                     /locus_tag="VEJ00_05920"
     CDS             complement(7169..8209)
                     /locus_tag="VEJ00_05920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXX00729.1"
                     /translation="MDSLSMKYTRRAFLSLALLIGTALVGQSFAQSDYASGAPSLSAY
                     NGASAATESTAGNSTPDSDEPQGALITLRARVNEVDVLFIATDKHGKFVRDLTQKDFT
                     ILDDHKPPQAILNFRRETDLPLHLGLLIDVSGSVDSRFDFEQEAATSFLQHTLRSGFD
                     KAFIVGFNSHSQMAQDFTDNVQLLSAGVHKLHDGGGTALYDAIYRACKEKFLKDHPEH
                     PVRKAIIIVSDGEDNQSEVSRAQAIEMAQRAEVIIYAISTDDSGLVMRGDRVLEQIAE
                     ATGGRVFFPFKMKDITHAFAAIEDELRSQYIVSYKPADFDADGRYRSIEISSLKKDLQ
                     VRARKGYFAPER"
     gene            8511..9221
                     /locus_tag="VEJ00_05925"
     CDS             8511..9221
                     /locus_tag="VEJ00_05925"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HXX00730.1"
                     /translation="MKKFIVCCLAVLISSALAFAGDEREEERVKDAGNVLKEILNIPD
                     DIPQDLLDKAECVIILPSVKKGAFGIGGSYGRGVMVCRSGAHYKGKWGAPALYALEGV
                     SIGFQLGGQATDFVLLVMNPKGARSLLSSKVKLGADASAAAGPKGRTAEGATDVVMSA
                     EILSYSRNKGLFAGISLEGSTLRSDGSANEKLYGRKLTAKDIIRDGKVGIPGCAHELV
                     SLLDTKTPVNKSDPKSLE"
     gene            9243..9842
                     /locus_tag="VEJ00_05930"
     CDS             9243..9842
                     /locus_tag="VEJ00_05930"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HXX00731.1"
                     /translation="MSSVAKGSRARSRCSWASGSELSIRYHDEEWGVPVHDDRTLFEF
                     LILEGAQAGLSWNTILNKRENYRKAFGGFDPERVARYDRRKMQQLMRDPGIVRNRLKI
                     RAAVENAKAFLRVREEFGSFDRYIWQFVGGKPRVNRWNKRSRVPARTDESDAMSKDLK
                     RRGFRFVGSTICYAFMQAVGMVNDHAVDCFRYKPLSSQR"
     gene            complement(9833..10315)
                     /locus_tag="VEJ00_05935"
     CDS             complement(9833..10315)
                     /locus_tag="VEJ00_05935"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HXX00732.1"
                     /translation="MSFLRFLMLLSLVVWIGGLIFFAFVLAPTAFTVLPSTHLAGDVV
                     GRALGKLHWIAIVAGIVFLISSLLYSRMTDGSGHVFALRHVLICLMLALTLFSQFWII
                     PRMDTLRAQVGDFAAVPLTNPARVQFDALHVWSTRVESAVLLLGLVVVYLTASAFAQR
                     "
     gene            complement(10366..10830)
                     /locus_tag="VEJ00_05940"
     CDS             complement(10366..10830)
                     /locus_tag="VEJ00_05940"
                     /inference="COORDINATES: protein motif:HMM:NF025471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4279 domain-containing protein"
                     /protein_id="HXX00733.1"
                     /translation="MPTKDPFRINIAIRHPSYTPQRISEALSMKPLWSHTAGDRFIGR
                     RKWKLTHFYALLQKGDYASQFERRLRKVVSFLKKHSAFLNALTKSNGEAEIILNHTIN
                     LQDEEGDKNFELSLAPAFVVCLASQRIGLRVQGWQGKAPRKRSGVRRRAKTR"
     gene            complement(10865..>11352)
                     /locus_tag="VEJ00_05945"
     CDS             complement(10865..>11352)
                     /locus_tag="VEJ00_05945"
                     /inference="COORDINATES: protein motif:HMM:NF036831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXX00734.1"
                     /translation="GTYPLPESQLDRFLLRVRMGYPDPQAEREILRSEAGAAQMDELR
                     PVLTGADVVEMQQAIKQIRVDESLVSYALEIVRRTRESEFLSLGVSPRGSQALYRAAQ
                     AMAFLEGRTFCTPDDFKSLVVSVFAHRVVVNGLYASTLKKSEQSDQVLHEIVENVAVP
                     V"
BASE COUNT         2367 a         3317 c         3407 g         2261 t
ORIGIN      
        1 aggccgcccc agccggcggc ctctttcaat ttccgacgat gatcttcagt ctactgcgca
       61 acgcctgccg gcggttgtgg tacggttcct gaagcagtcg cggcggcggc ctcgttcaga
      121 atgcggtggt ggatggtgaa cagcgccagt tccagccggt ccgacactcc gatcttgtcg
      181 tacacgttgc gcagatagtt ctttatgacc tgctcggtcg tgccgagttg cgtggcgatt
      241 tccttgttct tgtacccctg cacgatcaga gcgacgatgc ggagctcttt tgctgtcagg
      301 cgatcacgca cgcgcagtcc gaccatatcg ttttcggtga gttcgctggg tacggcgacg
      361 tcttgcaccc aggtctcgcc gcgggcgact ttccggacac aatcgacaag cgcggcactg
      421 gtcacgttgc gataaaccac gccatgggcg ccgttcgaca tgtgccgttg cgcactctcg
      481 cccgtgtcgg ccagcacaat cacgcgcgtt ttggccttgt ttgccgcctg cacaatggcg
      541 gcgaagtcgt tgtgaaatcc cgccgccacc accagcaccg cggcacggaa tttgtcgaga
      601 gccatgaaca tctgttcagg cgtttgcgcc tgagccacaa tgcgcatctc gtcttcgaca
      661 gcgaggactt tggcgatgcc cgcccggaag atcgcctggt tatcggccag aatcagtttg
      721 agcatttgtc agcgctccca aaaatccagt tgaaacgacg cggttgaaac tcttatgagt
      781 tcctcacgaa ctcgtaggtg tcgatgacgc aggccacgcc gcgcccttct ccgccagtgc
      841 cggtctcatc ggcgcgcacg acgcgtccct tgcactggat ggtcacgttt tcctgcccgc
      901 ctgtcatctc cgggggcagc gtgatttcga actcgacggg ggaaccgatc tccatggtgg
      961 cgtcggcgcg gatatacaca cccgccgcgc tcagattgcc cgtcacccct ttggcgtcag
     1021 tttcgccctt gtgaatcttg atcggcaatt ccagcgggaa ccgttttccg gtccgtgctt
     1081 cttcgcccac ggtgcctcct ccaagccggc gtgtgttcag tcggtcgtcg gccaatgcca
     1141 gcgtcaaatc aagctcctgg cgggcgtaag cccacttttg tgctcagcgc ggttatatca
     1201 cggaaagtta cccatgtaaa agcctgataa cccgttagtt accggtagca actaggttcc
     1261 cccatggcgg aaaatgttcg ttcccggctc agtcggttcg aggacgcaag aatagcgtcc
     1321 cgacctcacc caggtaaggg tcggtgatgg tgcgggcggc agacgaggcc gaggcatgtt
     1381 cgatcgtttc cacaatgtgg cgatacaccg ggctggcgga agatccccaa accgcaagcg
     1441 cgtccgcctt cgtggccagt gccttggcgg ccagtgtgtc ggcagcggca aggccgggga
     1501 gttctgcatt tgcggcgaca aggcgccatt ccgccagata ggccacgtag ggcgcggctt
     1561 cgaatcctac accgtcttgc ggatacggcc catcgccgat gctggccagc gtgccatgag
     1621 ggtttgcagc tcgcaagatg gcggcggcct ggtagcagct ggcttcgaaa tcgcgatcca
     1681 cggtgcgaaa cggcgaggcc cagtagacgg tgaagaaaac cgtgcccacg atcagaaggc
     1741 cggccgctgt ttttctttgc caaccgggga cgccgggcgc gtccggggcg aactctgcgg
     1801 gcagcaacgc cgggcagctg atcgctatga gcagcggcag aaccgggatt acgtagcgtg
     1861 cgtcgaaaac cagcatgcaa taggccacga tcacgctgag aacgcttagc agcgcgatgc
     1921 tgacaaaagc agcttgtgtc cggtactgag atctcttctt tgctagcgct gcgagcatcg
     1981 cggccagagc aagaactcct ccgggattca gcaggatccc ggtttccttc agagcctgag
     2041 gcagattctg gaattcgcta cccagaatca tgctcagcat tccaggtctg cgcaggctga
     2101 agccctgcac agcggccaag ggagactccc cgaccatgta gtggtcgtac accgaggagg
     2161 tgtccacaaa tgccagattg tctccgcgcg ggcggtgact caatcttctc ttccaattga
     2221 tcatgagatt ggtgcggagc tggtcgccag tggtgaattg cccgtatttc gagcgcaggg
     2281 cgagacccca accgatccac accgccgccg gaaacagaaa cacaagcagc aacgacgcag
     2341 tgagccggcg cggcgaccgg gcgttttgca cgagcagcgc cagcaccgtc gagatggcta
     2401 gccagggcat ggcaatcgct ttcgccagga aggtcaaggc atgagccgcg cccaccgcca
     2461 accagcctac aggccggtcc tgccgcagca cgcgcagaag aaggacgaag tacaggagta
     2521 cacaagccgt gagcacgaaa tctgccagaa tcaatcccac ggccgtggct gccactcccc
     2581 gcgccgtgga gaaccacaac accgacaagg ctgcagccac ccgggagcgc cacagcttca
     2641 gcgtcagcag atagagcaac ggagggcaga gcaggaagct ggtgatcgtc accagtcttc
     2701 ccaacagcgt gaaatttctg ccgaacagcg cgcttgcggc gatgatccat gaggtcaacg
     2761 ggctccagta accgtgcagc gaggctttga cgtctccgtc gacaaggtgg cgagccagtc
     2821 cgatgtaggc gatcccgtcc atcgtgatgt tgtgggcacg aaagcgccag aaccaggcga
     2881 tttgaaatag acagctcgca gcggtcgctg cgagcagagt gatccaacag actttttcag
     2941 atcggtgttg tggttttgac tccgcgagct ggaccgtcgg cgggatcatg ggcaaggtgt
     3001 gattgtagca actgccctct gccagagcgg aagtgcgagt tgtgctcgac cttgccgggg
     3061 agtacgcgaa agcatgggag tgcatgattc agatatgctg tgaggcaaag ggagcaatcc
     3121 tatgacgact tctttcccac gccgcttccc cgccaatctt cggctcattc tcgcttcttg
     3181 cttgatcgtg ttgatctctc gcacgagtgg cttgtcgcaa actggcgggg ccaaacccgc
     3241 ccccaccaca cagaaaaagg gcggcggcgc gacggagacg aaagcctcgg aagcaccaag
     3301 cgttctcgtt cagctgaatg gagctttgga aggtctggca gccaaggtgt cgcctgcagt
     3361 cgtacagatt ttggttacgg gatacgggcc cctgaaagag gaagatcgat cgcagacggc
     3421 attgatcgtt cgccagcatg cggtgggctc gggcgtgatt gtcgacccca atggctatat
     3481 catcaccaac gcccacgtag tggaaggggc acaacgcatt cgcgtggcac tgccgctgcc
     3541 gatgggtgat tcttccggcc aggtgccgat cggaaagcgg aggattctgg aagcgcgact
     3601 cctcggccag cacaaggaaa ccgacctggc cctgctcaaa atcgaggaaa ccgacctgcc
     3661 gacgcttgca cttctgaccc agcagcgccc gcacgtgggg caactcgttt ttgctatcgg
     3721 tagcccggaa ggcctgcaga attccgtcac catgggtgtc gtcagcgcgg tggcccgaca
     3781 agtcgatcct gagaagccgt tgacctacat tcaaactgat gcgcccatca acccgggcaa
     3841 cagcggaggg ccgctggtgg atatgaatgg ggcggtcctg gggatcaaca cgttcattct
     3901 gtcgcaggga ggaggaagtg agggcctcgg ctttgccatc ccggcccgca ttgtcgagtt
     3961 cgtttaccac agcctgcgcg cgtacgggca cgtgcatcgc gtcgaaattg gcgccgcggc
     4021 gcaggagatc acacccactc ttgccgatgc cctccatttg ccacggcatt ggggcgtcat
     4081 cgtggatgac gtccgccccg gagggcccgc ggctgcggct ggactgcaga ttcaagacat
     4141 tattctcacc gccgatgacc gccggattga aactctgccc tcgctctcag ccgcccttta
     4201 tctccaccgg atcgaccaag tgataaaact ggaaattctc cgcggcgagg aacggaaaac
     4261 cttgtacgtt cccgcgattg agcaccatga tcagatggat caattgctgg acgccgtcaa
     4321 tcccgcaaac agtctgattc cacgcctggg cgttttaggg attgatctga cgcccgacct
     4381 ggcctcgcat ctgggctcgc tccgcatcgc ttcgggcgtg atcatcgtgg ggcgcgccgc
     4441 cgacctcatc attcctgaca ctggtctgca ggcaggatac atcatccacc ggttcaacgg
     4501 cgcaccgatc gattcgatgg aagcactacg cagcgcggtc gcagcgctga agagcggaga
     4561 tccagtcgcc atgcaggtgg agcgcgatgg tggcctgacc tacgtgagct ttgaggtcga
     4621 gtagttcacc ctgctgactg ggctttcgaa tcatccgcaa gaacccccgc tcggcgccat
     4681 gttcggcgca gctagcgggg gcaccccaat caccccacgc ctgcaatcca attctaaggt
     4741 tcagtcgtac ttccagtgaa cgcaaattga ccgccatgtc cgaaaacaga caaacactcg
     4801 aattcgacgt agagaaatac attcgcaact cgaagaaagt cgacatcagc gatctcgacc
     4861 tcgcgcaggc gtcgcgctat cccgtgtccg aagacgaagt tcgcgcgctg acctacatgt
     4921 gcgacatcga atcgcacacc atcgtctacc tgcgggccat tctgaatacc tgcgcagtag
     4981 aagatccgca gacgaccagc ttcctgagtt gctgggctta cgaagagttc ttccacgggc
     5041 aaacgattcg ccagtttctg gacgccatcg gcgcgccggt gtcttcgacc cgcatgctgg
     5101 aagtgcaaaa agccacttcg ttccacgagt ggctgaagga gttagggtcg tccattctgt
     5161 gccgcttctc gcgccacttc catggcgctt acctgaccta tggcgcgatc tcggaactgt
     5221 cgactctgga aggttatggc gtcatggcgc ggcggacaca gaatccaatc ctggcggaga
     5281 tcgtccgccg cctggccaag gatgaacgac ggcatttttc cttctactac aacaaggcaa
     5341 ggcttgagct gcaggcgaag aacgcgcagc ggctgaccaa tttcgtgatc cggaacttct
     5401 ggctacccgt gggcggggga gtaaagccag acagcgaggt gagctggatc ctctcgttca
     5461 ttctgggtga tccggaaggc gccgagatcg cgagccggat cgatactgcg atcgcaaagc
     5521 tcccgggcct cgattggttt gaccgcctgt cgcgcagccg tgctgaggcg ctacaagaaa
     5581 cggggatgca acccgcacaa gagatacgaa cggcagttcg attcttcgcg gaaccgatct
     5641 gaccctgatg ctcagcggct aatttgtaac agcaaccttg cccaacgccg ccttcagttc
     5701 ctccgggttg aagccaaagg ggccggcctt gctcttgtag acgaccttgc cgtggccgtc
     5761 gatcaggtaa aggcgatctg gccatgcggt ataggcgctt tccgtcgagt ttccgaactc
     5821 gtcgagaacg gcagggacct cgattcccag cttgcgtacg cacgcgcctg ccacgaaggc
     5881 acgctcttct tcgttgcgcg gactggcgaa aaccacgttg tccttgagat tgctctgcat
     5941 ctgccagacg tcactcggat gcgcctcgct gatgtagacc accaggaatg caacctggtt
     6001 ctgatactgc tcgtagagtt tgttgagggc aggaacctcc cgccggaatg gcggtcaggt
     6061 gtagcttcca aagatcagcg ccacgggctt gccttgcgcg gtcagggatg acagttgcac
     6121 ctgcgcgctc ttatccagct tcgtgagctt gaaatcgggg gcagagtcgc cgacggcgag
     6181 ctttccggtc cgcgcgcgag tccacagcgt ttcgaaagga aagaccagaa acgcaaccgg
     6241 cgccggaatt ctggacatga accggccgaa gctttccgga ggctggcgca tggcatggta
     6301 gattgcgccg caaccgagca gccacagcac cgcgacaacg gagaaggtag cggccaaagc
     6361 cttcttcact gacctgggtt tttggttcac aagaatcgaa ccccaaaaag aagtgcgccg
     6421 attatagggg cagggaaagg aagatcaata gtgggaaagc agtatccgcc tgcccgcgac
     6481 ctcaaaccac tacactgctc gaggccaaga atttgccaat agctagaact tcacctgcac
     6541 cggctcacgg tcggcggccg tggtctcgaa gaactgacgc ggggtgaaac tgaacatgcc
     6601 ggcaagaatg ctggccggga aagactgaat ctgcgtgttc agatcgcgaa ccgcggcgtt
     6661 gtagttgctg cgcgcgttct ggatcgaatt ttccgtctgg ctcagcgaac cctgcagttg
     6721 catgaactcc tcggaggcct tgagctccgg ataactctca gccacagcaa acaggctttt
     6781 caaggcgccg gtcaggagat tctcggctgc tgccttgtcg gccggggtcg cagcctgcat
     6841 cgcctgcgag cggaacttgg caacgttctc gaaggtgccc ttctcgtgtg cggcgtatcc
     6901 cttgaccgtt tccaccaggt tgggaatcag gtcatgccgc cgcttcagct ggacatcgat
     6961 gtcggaccag gctgaatcgc aactcacccg cctctgcacc agtccgttgt acattccgat
     7021 caacacaacg gcggcgacaa tcaaaatgag caggatgacg atgataaatg tcatgaggcg
     7081 cctctccact cagaaagcgt ggacgcgaat tccatcggac gttagcacag ggggagcgct
     7141 ctcgcaatga tcggaaacca agcccgcttt accgctctgg ggcaaagtat cccttgcggg
     7201 cccgcacttg cagatccttc ttcaaagagg agatctcgat gctgcggtag cggccgtcgg
     7261 cgtcgaagtc cgcgggcttg taggaaacaa tgtactggct gcgcagttcg tcttcgatcg
     7321 cggcaaaagc atgggtaatg tctttcatct tgaaggggaa gaaaacccgg ccgccggtcg
     7381 cctcggcgat ttgctccaga acccggtcgc cgcgcatcac gaggccgctg tcgtcggtcg
     7441 agatcgcgta gatgataacc tcggcgcgct gtgccatttc gatggcctgc gcccgtgaaa
     7501 cttcgctctg gttatcttcg ccgtcgctga cgatgatgat ggcttttcgt accggatgct
     7561 ccggatggtc cttcagaaac ttctctttgc aggcgcgata gatggcgtcg tagagggcgg
     7621 tgccgccgcc atcgtgcagc ttgtggaccc cggccgacaa cagttgcacg ttgtcggtga
     7681 agtcctgcgc catctggctg tgactgttga atccgacgat aaatgccttg tcgaagccgg
     7741 agcgcagggt gtgctgcaga aaactggtcg ccgcctcctg ttcgaagtcg aagcggctgt
     7801 cgaccgatcc gcttacgtcg atcagaagac caagatgtag aggcaggtcg gtttcgcggc
     7861 ggaagttgag aatggcctgc ggcggcttgt ggtcgtcaag gatcgtgaag tctttctgcg
     7921 tcaggtcgcg cacgaacttg ccgtgtttgt cggtcgcgat gaacagcaca tcgacttcgt
     7981 tcacgcgcgc acgcagagta atgagcgcgc cctgcggttc atcagaatcg ggcgtagagt
     8041 tgcccgccgt gctttcggtc gcagcgctgg cgccgttgta cgcgctcaga ctgggcgctc
     8101 cgctggcgta gtcggattgg gcgaacgact ggccaaccaa ggccgtccct atcaacaagg
     8161 ccagagacaa aaatgcgcgc cgcgtgtact tcatgctcaa tgagtccaaa tttttcactc
     8221 taaaccgtgc tttgcacact gccaaactca taggatgcac gccagatgcc gaaccctaag
     8281 attcgtcaga tgccgctatc tgcttgactc taggagggtg ccgccgatgt gccaagttac
     8341 aaaaggagca cacgtttggt catggtacaa aacgtgcgga ttcttgcagt tacggcatgg
     8401 gtaggaggtt cattccgccc gtcaccagcc gggttcgggg tttacgatag tatgcgccgc
     8461 ggaccggcat ttccttacaa tctttcttgc aatccctgcg gaggaaggtc atgaagaaat
     8521 tcatcgtgtg ctgtcttgct gtgctcatct cctcggcgct ggcgttcgcg ggcgacgaac
     8581 gcgaagaaga gcgcgtcaaa gatgcaggca atgtcctgaa ggagattctg aacattcccg
     8641 atgacattcc tcaagacttg ctcgacaagg ccgagtgcgt gatcattttg ccctcggtaa
     8701 agaaaggagc gttcggcatt ggcggcagct acggacgcgg cgtgatggtg tgtcgcagcg
     8761 gcgcgcacta caaaggcaag tggggagctc cagcactgta tgcgctggag ggcgtcagca
     8821 tcggatttca gctcggcggc caggccaccg acttcgtgct gctggtgatg aatcccaagg
     8881 gcgcgcgctc gctgctttcg agcaaggtca agctgggcgc cgacgcctct gccgctgccg
     8941 gcccgaaggg acgcacggcc gaaggcgcca ctgacgtggt catgagcgcg gagatccttt
     9001 cgtactcgcg caacaagggg ttgtttgccg gtatttctct ggaaggttcc accctgcgct
     9061 cggacggcag tgcgaacgaa aagctgtatg gcaggaagct gactgcaaag gacatcattc
     9121 gcgacggcaa agtcggtatt ccgggatgcg cgcatgaact ggtgtcgctg cttgatacca
     9181 agacgccggt gaacaagtcg gatccgaagt cgctcgaata aatctcgtct ctgtgagata
     9241 ccgtgtcctc tgtggctaaa ggttctcgtg cgcgctctcg ctgcagctgg gcgagtggca
     9301 gcgaactctc cattcgttat cacgatgaag aatggggcgt gcccgtgcac gacgatcgca
     9361 ccctgttcga atttctcatt cttgaaggcg cgcaggccgg gctgagctgg aatacgatcc
     9421 tcaataagcg cgagaactac cgcaaggctt tcgggggctt cgatcccgag cgtgtggcgc
     9481 gctacgaccg gcgcaagatg cagcagctga tgcgtgatcc cggcatcgtg cggaatagac
     9541 tgaagatcag ggcggccgtc gagaatgcga aggctttctt gcgcgtgcgg gaagaattcg
     9601 gcagtttcga tcgctacatc tggcaattcg ttggcggcaa gccgcgagtg aaccgctgga
     9661 ataagcgcag ccgggttccc gcgcgcaccg acgaatccga cgccatgagc aaagacctga
     9721 aacgccgcgg cttcagattc gtcggctcga ccatctgtta tgccttcatg caggcggtcg
     9781 ggatggtgaa cgatcatgcc gtggactgct tccgctacaa accgctttct agtcaacgct
     9841 gagcaaaagc gctcgccgtt agatacacca cgaccagccc caacagcagc accgcgctct
     9901 ccacgcgcgt cgaccagacg tgcagcgcat cgaactgcac gcgcgcggga ttggtcagag
     9961 gaactgcagc gaagtcgccg acctgcgccc gcaacgtgtc catgcgcggg atgatccaga
    10021 attgcgagaa cagcgtaagc gccagcatca ggcagatcaa aacgtgtcgc agggcgaaga
    10081 catgcccgct gccgtcggtc atacggctgt agagcaggga actgatcagg aacacgatgc
    10141 ccgcgacgat ggcgatccag tgcagtttgc cgagggcgcg tccgaccaca tccccggcaa
    10201 gatgcgtgct gggcagaacc gtgaaggccg tgggcgcgag cacgaaggca aagaagatca
    10261 acccgccgat ccagaccacg agcgagagca gcatgagaaa gcgcaggaag gacatgacgg
    10321 cggaattata gtcgcaggtc gtgcgtgggt gtcggacgaa agaagtcagc gggttttcgc
    10381 cctgcggcga actcctgacc ttttacgggg agcttttcct tgccagcctt gtactcttag
    10441 gccgatgcgt tgagacgcca gacagactac gaaagctgga gcgagagaaa gctcaaagtt
    10501 cttgtctcct tcctcatctt gcagatttat tgtgtggttc aaaatgatct cggcctctcc
    10561 attgctctta gtcagggcat tcaaaaaggc ggagtgtttc ttgaggaatg agacgacttt
    10621 cctcagtcgc ctttcgaact gtgacgcata gtcacccttc tgcagcagcg cgtagaagtg
    10681 ggtcagcttc cacttgcgtc taccgataaa tctatcgccg gctgtgtggg accaaagggg
    10741 cttcatcgac aaagcttcac taatgcgctg aggtgtgtag gaaggatggc gaatagcaat
    10801 gttgatcctg aatggatctt ttgtgggcat ttctcaattc tatcgaagag gctcagagca
    10861 ggagctacac cggcacggcc acgttctcca cgatctcgtg caggacttga tccgactgct
    10921 cggacttctt cagcgtcgag gcgtacaggc cgttcaccac aacgcggtgg gcaaagacgg
    10981 acacgaccag cgacttgaag tcgtcgggag tgcagaaagt gcggccttcg aggaacgcca
    11041 tcgcctgcgc ggcccggtaa agcgcttgcg agccgcgggg cgagacgccg agcgagagga
    11101 actccgattc gcgggtgcgg cgcacaattt cgagcgcgta gctgaccagg gattcatcga
    11161 cacggatctg cttgatcgcc tgctgcattt cgaccacatc agctccggtc agtacagggc
    11221 gaagttcgtc catctgcgct gcgcccgcct ccgagcgcag aatctcacgc tcggcctgcg
    11281 ggtcgggata tcccatacgc acccgcagca aaaagcggtc aagttgtgac tcgggcagcg
    11341 gataagtccc gt
//
LOCUS       DASPUL010000235         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1686891, whole genome shotgun sequence.
ACCESSION   DASPUL010000235 DASPUL010000000
VERSION     DASPUL010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686891"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VEJ00_05950"
     CDS             complement(<1..1374)
                     /locus_tag="VEJ00_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HXX00735.1"
                     /translation="MARLACYLAGLAAVLFALQKVLAMFAPSWGEHLGGWVGFLGFLA
                     VAFFSILAFQWLKRRILWRLRNRLIVTYVFIGVIPAVLLVGMALITLYGLAGQFAVFV
                     VTSEIHAQLRSLEAVNAAVSNELAARLERGANPAAESLAGLKKRDPAWGRRQVCAWYG
                     GKSLPLCDEFRGAALTAPRFSEGKFRDIVRDGDGLHLRVVNSLQVGSEQLTVVTSEPF
                     DKELVGKIAADLGEITLYGSGFGSEESSSKGDSLAKTNGQPTPRQGSGPHINVNLNPP
                     KNKGGIVITQSNGSAAQADQRVLQPSFTVGALAGPAGSFDSKIDFGTPLPVVDWKTGE
                     HEQVGAVVQVSTRPSVLYGHLFASLGEFVRGVAYILLGVAIFFAIIELVALFIGTRMT
                     RTVTAAVAQMHEATKYIDRGDFSHRIPVKSSDQLADLAISFNSMTASIEKLIQEQKEK
                     QRLENELA"
     gene            complement(1504..1704)
                     /locus_tag="VEJ00_05955"
     CDS             complement(1504..1704)
                     /locus_tag="VEJ00_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00736.1"
                     /translation="MANLTLVYGMRLLPSEDVAEVGQAPVKLANGREAHVTMHVLEGS
                     KEEIEKQLRTSLEAFFDLYPEI"
     gene            1865..2632
                     /locus_tag="VEJ00_05960"
     CDS             1865..2632
                     /locus_tag="VEJ00_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00737.1"
                     /translation="MPRILVSALPFILLGLPVALPAQEQPANPPANQPQVKVNMLNVC
                     TPSAEEQKEIASALARVPKQPLFSTDFEVSRGRSTLTDMPSFVPVEQRAHMAGEPSVA
                     NYVRIRREFSVQALFASVQYSFSNDGQNMIETLVLHVREPKDLIMVSLEDTASSVTSA
                     EAMLTANTPASRVRLERFGKSSIALARCSAAEGGQPVDQSAYEPLFRGASDVLANYRG
                     LLSVRSVVPEELAKLGGESKPKAAPKAKKAGPAADKK"
     gene            2713..>3116
                     /locus_tag="VEJ00_05965"
     CDS             2713..>3116
                     /locus_tag="VEJ00_05965"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXX00738.1"
                     /translation="MSETRNVVIIGSGCSGHTAALYTARANLKPLVIEGHEPGGQLSL
                     TTLVENFPGFPEGIMGPQLVENMRKQAERFGAEYRMAHLSSADLSKRPFLLHLGHQQV
                     HTQTLIIASGASARWLGLPHEQQLIGHGVSSCA"
BASE COUNT          631 a          987 c          874 g          624 t
ORIGIN      
        1 ggcgagttca ttctccagcc gctgcttttc cttttgctcc tggatcagct tctcgatgga
       61 ggcagtcatg gagttgaagg aaatcgccag atcggccagt tgatcgctcg atttcaccgg
      121 gatgcggtgg ctgaagtcgc cgcgatcgat gtatttggta gcttcgtgca tttgcgctac
      181 agccgcagtc accgtgcgcg tcatgcgagt gccgatgaac agcgcaacca gttcgatgat
      241 cgcgaagaag atcgccacac ccagcagaat gtaagcaact ccccgaacga actctcccag
      301 agaagcaaac agatgtccgt acaagaccga cggccgcgtg ctcacctgta cgactgctcc
      361 tacctgctcg tgctcgcccg tcttccagtc cactaccgga agcggagttc cgaaatcgat
      421 cttgctgtcg aaactgccgg ctggcccagc cagagcgcca accgtaaagc tcggctggag
      481 cacgcgctgg tcagcctgcg cggctgatcc gttgctttgc gtgatgacaa ttccgccctt
      541 gttctttggc ggattgaggt tgacgttgat gtgcggaccg gaaccttggc gaggtgtggg
      601 ctgcccgttg gtttttgcca gtgagtcgcc ttttgacgac gattcctctg acccaaatcc
      661 tgacccatag agggtgatct cgcctaggtc ggcggcaatc tttcccacca actccttgtc
      721 aaacggctcg ctggtcacaa cagtaagctg ctcggacccc acctgaagcg aattgacgac
      781 acgcaggtga agaccgtcgc catcgcggac gatgtcacga aattttcctt ccgagaaacg
      841 tggcgccgta agggccgctc ctctgaactc atcgcagagc ggcagcgact ttccaccata
      901 ccaggcgcag acctgcctcc gtccccaggc cggatcccgt ttcttcaatc cggccagcga
      961 ctccgcggca ggattagcgc ctcgttcgag ccgcgctgcc agttcattgc tgaccgcggc
     1021 gttcactgcc tccaggctcc tcagttgcgc gtggatctcc gaggtcacca caaacacggc
     1081 aaattggccg gccagaccgt acagagtgat cagggccatc ccgaccagga gcacggctgg
     1141 aattacaccg atgaatacgt aagtgacgat caggcgattg cgcaggcgcc acaggattcg
     1201 gcgcttcagc cactgaaatg ccaggatgga aaagaacgcg acggcgagga atccgaggaa
     1261 ccctacccag cctcccagat gttcgcccca ggacggagca aacatcgcca gcaccttctg
     1321 caaggcaaac aacactgcgg cgaggcctgc cagatagcac gccaggcgtg ccatgcggcc
     1381 ttcgggccac agccccgccc gtaccagccg gctccgaact tctgaatagg accaatgcat
     1441 gctgtttagt gaagccgttc gcaggaggat taggattata cggccagaaa ctgccgctcc
     1501 cagctagatc tcaggataca gatcgaagaa ggcctccagg ctcgtgcgca actgcttttc
     1561 gatctcttcc ttgctgcctt cgagcacatg catggtgacg tgggcctcgc ggccattggc
     1621 cagtttcacc ggagcctgac ccacttcagc cacatcctcg gagggcagca gtctcatgcc
     1681 ataaaccaac gtgagatttg ccatagctga attctaagtc tttccgtgcc atcacggcag
     1741 cctttcccaa ccaccgaagg cgtagagtgc gcgcctcact tcccgtactc cctttacgtc
     1801 ctttgcagta gctcttcgcg aactttgcgg taagatcttc ttcgttctca tatagaattc
     1861 cgccgtgcct cgaatcctgg tctctgcact cccatttatc cttctggggc tcccagtggc
     1921 gcttccggcc caggaacagc ccgcgaatcc ccccgcgaac cagccacagg tgaaggtcaa
     1981 catgctgaac gtgtgtacgc cgtcggccga ggagcagaaa gagatcgctt ccgcgctggc
     2041 tcgcgtcccc aaacagccct tgttcagcac cgattttgag gtgtcgcgcg gccgttccac
     2101 gctcacagac atgccgagct tcgtgcctgt cgagcagcgg gcgcacatgg ccggcgagcc
     2161 ttccgtagcc aactacgtgc gcattcggcg cgaattttcg gtgcaggcgt tgttcgccag
     2221 cgtgcagtat tccttcagca atgacgggca gaacatgatc gagacgctgg tgctgcacgt
     2281 acgtgaaccc aaggacctga ttatggtctc gctggaagac accgcttctt ccgtgaccag
     2341 cgccgaggcc atgctcactg ccaacacgcc cgccagtcgt gttcggctgg agcgctttgg
     2401 caagtcttcc atcgcgctgg ctcgctgttc ggcggcggaa gggggtcaac ccgtggatca
     2461 gagtgcctac gaaccgctgt tccgcggcgc ctcggatgtg ctggcgaatt atcgcggcct
     2521 gttgtcggtg cgttcggtcg tccccgagga attggcgaag ctcggcggcg agtcgaaacc
     2581 aaaagctgcc ccgaaggcga agaaggccgg gcccgcggcg gataagaagt gactggacat
     2641 tccctgtcgg ctgcgatcaa acgcgcagtg catccaaatc tgaactgagc catctaatct
     2701 gcaacgaaat ccatgagtga aactcgcaac gttgtgatta tcgggtccgg atgctccggc
     2761 cacacagcgg ccctgtacac cgcacgcgcc aacctgaaac ctctcgtcat tgaaggccac
     2821 gagccgggcg gacagctttc tctcaccacg ctggtggaga acttccctgg attcccggaa
     2881 ggcatcatgg gcccacagct ggtcgagaac atgcgcaagc aggcggagcg cttcggagcg
     2941 gaataccgca tggcgcatct cagcagtgcc gacttgagca agcgcccttt cttactccat
     3001 ctcggccatc agcaggttca tacccagaca ctgatcatcg ccagcggcgc ctcggcgcgg
     3061 tggctggggc tgccgcatga gcagcagctc atcgggcacg gtgtctcctc gtgcgc
//
LOCUS       DASPUL010000236         4057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_723870, whole genome shotgun sequence.
ACCESSION   DASPUL010000236 DASPUL010000000
VERSION     DASPUL010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723870"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VEJ00_05970"
     CDS             complement(<1..921)
                     /locus_tag="VEJ00_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXX00739.1"
                     /translation="MSSDDSRELHWAFGFRLQTWPLRYGFALLAVSLATLLRYALGVA
                     VGLRPPFLLFYPIIIVTALLAGLGPGLAATLSSAVSGAYFFHAQVVGTTGNGGAALYV
                     LLFLGMGTVLSLLADRVHRHQGHRRKLEKLVEGLDEMAVVVDRNYRYVLANGAFLSRR
                     GMTREQVIGRQVDEILNPGVFEAEVKEKLDRCFQGQIVQYEMRYTYPSLGERDLLISY
                     LPIEGPTGVERAACVLQDTTDRKRAERLFRTMIDPSNDAVELVDAETLRFLDFNEKAC
                     NDLGYTRDELLALTVFDINPTVNEAKRASVT"
     gene            complement(1245..2582)
                     /locus_tag="VEJ00_05975"
     CDS             complement(1245..2582)
                     /locus_tag="VEJ00_05975"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXX00740.1"
                     /translation="MTTATATPTMTGKEMVDLTKKHTLYEWSAQSKVDPIPVAKAKGI
                     YFWTPEGKRFIDFNSQLMSVNIGHGDERVIQAIAEQAATLCYANPFMAHEPRARLGAK
                     LAEITPGDIDTFFFTNGGAEANENAIKLARFFTGRQKIIARYRSYHGATAGVISLTGD
                     PRRWAAEPGIPGVVRVLDPYHGIERGWESAESSLATIEETIQLEGPHTIAAFILETVV
                     GTNGILIPPDGYLQGVRKLCDKYGILMIADEVMAGFGRTGEWFAIDHWKVVPDLISMA
                     KGLTSSYLPLGAVGMRHHIAQHFQDKVFYGGLTYNSHPMGCAAALATIRVYEEDNLIE
                     NAKRMGTVMNRLGKEMQAKHPSVGDYRNIGLFGIFELVRSRKTRQPMAPFNGTSEEMG
                     ALGKFFREQGLYTLVRWNTFFTNPPLCINEQQLREAFAIIDKGLELADRAVKD"
     gene            complement(2643..3188)
                     /locus_tag="VEJ00_05980"
     CDS             complement(2643..3188)
                     /locus_tag="VEJ00_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXX00741.1"
                     /translation="MSLQVQIEDGVDHRGRMVRSDRQMSDEDTREYLRQHCVAHVGST
                     DASGWPYVVPLMYVYEGGELLYLHTGPHQGHFLANVRETGRICLQLDESGPWHRNQPT
                     AFDSSLIYKSVIVFGKVRVMQDPGLTEKKVWFFDRLLERLHDNRSNYEKSYPTAMLER
                     IHLYEVELEIVTGKRNVVLGH"
     gene            complement(3196..>4057)
                     /locus_tag="VEJ00_05985"
     CDS             complement(3196..>4057)
                     /locus_tag="VEJ00_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXX00742.1"
                     /translation="GGKGVVDALIDHPKVRAISFVGSTPVARYIYARSAEKGKRCQCQ
                     GGAKNHVIVLPDADMPMATQIISDSAFGCAGQRCLAVSVAVTIGEAQKTFRDSIADAA
                     SKLKVGNGLEDGVQMGPVITSQSKERVEHLIGVGEKQGAKVLLDGRNTRIPKYESGNF
                     VKPTILDDVPATSEITDTEIFGPVLSLVHADSLDEGLAFLERSTYGNQASLFTSSGSA
                     ARRFRYEAPAGNIGINIGVAAPMAYFPFSGWKNSFFGDLHGQGRDAIEFYTDKKVVVE
                     RWAKEHSRKF"
BASE COUNT          834 a         1213 c         1132 g          878 t
ORIGIN      
        1 cgtaacgctg gcacgctttg cttcattgac ggttggattg atatcgaaca cggtcagggc
       61 aaggagttca tcgcgcgtgt agcccaggtc attgcaggcc ttttcattga agtcaaggaa
      121 ccgcaaggtc tcggcatcta ctaactccac cgcatcattg gatgggtcaa tcatcgttcg
      181 gaacagccgc tccgcccgtt tccgatccgt tgtgtcctgc aaaacgcatg cagcgcgttc
      241 gaccccggtc ggaccttcga tgggaaggta ggaaatcaga agatcgcgct cgccaagtga
      301 cgggtacgtg taccgcattt cgtactgcac gatctgtccc tgaaaacacc tgtccagttt
      361 ctctttgacc tcggcttcaa acactccggg gttaagaatc tcgtcgactt gacgtccgat
      421 aacctgctcc cgcgtcattc ctcgtcgact caagaacgca ccgtttgcga gtacgtagcg
      481 gtagttccgg tcgaccacca ccgccatctc atctagtcct tcgaccaatt tttctaattt
      541 ccgcctatgg ccttgatgac ggtgcacccg gtcggcaagc aagctgagaa ctgtccccat
      601 acccaaaaac aagaggacgt agagcgccgc gcccccattt ccggttgtcc cgactacctg
      661 tgcatggaaa aagtaagcgc cggaaacggc cgacgagagt gtagccgcaa ggcccggacc
      721 gagacccgcc agcagcgccg tcactatgat gatcggataa aacagcagaa acggcggtct
      781 cagcccgacc gcgacgccca acgcatatcg caggagggtg gccaaagaca cagccagcag
      841 ggcaaagccg tagcgaagcg gccatgtctg aaggcgaaac ccgaacgccc aatgcaattc
      901 cctggaatcg tcgctactca tcgccaactc aatacgtcag attccccagg gtaatttcaa
      961 caaccagccg gtgagactta catcacgtct ggctacgtcg gacacaccac atttgtccaa
     1021 gcccgagcag cgtccagcat tatgccacgg cacgccattg tctgtaagcc caacataggt
     1081 cacgacaagt ctcaaaatgg gaaagattga tcactcaaat cggaggagaa gttggcagtg
     1141 gcggactcca gagggctgga tccgaaccga gttcgcaggc ggaataaaaa aggcctgccc
     1201 tcgcgctcgt tgcgcaaaga taggcctctt atcgctctgc gtaactagtc cttcacagct
     1261 cggtcggcaa gctcgagccc tttgtcgatg atcgcaaagg cctcgcgcaa ttgctgctcg
     1321 ttgatgcaca acggagggtt ggtgaagaac gtattccagc gtacgagcgt gtacaggccc
     1381 tgctcgcgga agaacttgcc cagcgcgccc atctcttcgg aagtgccgtt gaacggcgcc
     1441 atcggttgcc gcgtcttgcg gctgcgcaca agttcgaaga ttccgaacag gccgatgttc
     1501 cggtagtcgc cgaccgaggg atgctttgcc tgcatctctt tgccgagccg gttcataacc
     1561 gtgcccatcc tctttgcgtt ttcgatgagg ttgtcttcct catacacgcg aattgtggcc
     1621 agtgcagcgg cgcaacccat cgggtgagag ttgtacgtca gcccgccgta gaagactttg
     1681 tcctggaaat gttgcgcgat gtgatggcgc attccgaccg cgcccagggg cagatagctt
     1741 gatgttagcc ccttggccat agaaatcagg tcgggaacaa ctttccaatg atcgatggcg
     1801 aaccactcgc ctgtgcgccc gaatcccgcc atcacttcat cggcgatcat gagaatgccg
     1861 tacttgtcgc acagcttgcg cacgccctga agatagccgt caggcggaat cagaatgccg
     1921 ttggtcccga cgaccgtctc aaggatgaac gcggcgatgg tgtgcggccc ttcgagctga
     1981 atcgtctcct cgatagtggc aagcgaggac tcggccgatt cccatccacg ctcaataccg
     2041 tgatacggat cgagcacgcg cacgacgccg ggaatgcccg gctcggcggc ccagcgtcgg
     2101 ggatcgcctg tgagcgaaat gacgcccgcc gtcgcaccgt gataggagcg gtagcgggcg
     2161 ataatcttct gccggccagt gaaaaagcgc gccagcttga ttgcattctc gttggcctcg
     2221 gcaccgccat tggtaaagaa gaacgtgtcg atgtctcctg gcgtgatttc ggcgagcttc
     2281 gcgcccagcc gtgctcgcgg ctcatgcgcc atgaacggat tcgcgtagca cagcgtcgcc
     2341 gcctgctcgg cgatggcctg gatgacgcgc tcgtcgccgt gaccgatgtt caccgacatc
     2401 agctggctgt tgaagtcgat gaagcgcttt ccttccggtg tccagaaata aatgcccttg
     2461 gccttggcga ctggaatggg atcgactttc gattgcgccg accactcgta cagtgtgtgt
     2521 ttcttggtga ggtcgaccat ttccttcccg gtcatggtcg gagtagcggt tgcagtagtc
     2581 atggtaagac tccctgttac taagaattcg gttgcatgaa tcgagattgc aacattcgat
     2641 agctagtgac cgagcaccac gttccgcttg ccggtcacga tctccagttc gacttcgtag
     2701 agatgaatcc gttccagcat ggcggtggga taagactttt cataattcga tctgttgtca
     2761 tggagccgtt ccaaaagccg gtcaaagaac cacactttct tttccgtcag ccccgggtct
     2821 tgcatcaccc gcactttgcc aaagacgatc acactcttgt aaatcagaga cgagtcgaac
     2881 gccgtgggct ggttgcgatg ccagggcccg ctttcgtcaa gctgcaggca aatgcgcccg
     2941 gtctcgcgaa cgttggctag aaagtgtccc tgatgcggcc ccgtatgcag gtacagcagc
     3001 tcgccccctt cataaacgta catcagcggg acgacgtagg gccaccccga ggcgtcagtg
     3061 cttccaacgt gcgcgacaca atgctgccgg agatattcac gagtatcttc gtcagacatc
     3121 tggcgatcgc tgcgcaccat gcgcccgcgg tggtcgactc cgtcttctat ctgtacttgc
     3181 agactcatcc ctccctcaga atttccggct gtgctctttg gcccagcgtt cgacaactac
     3241 tttcttgtcg gtatagaact cgatggcgtc ccgaccctgt ccgtgcagat cgccgaagaa
     3301 gctgtttttc catccgctga acgggaagta cgccatgggc gcagcgactc cgatgttgat
     3361 cccgatgttg ccggcgggtg cttcatagcg gaatcggcgc gcggcggatc cgctagacgt
     3421 gaacagcgac gcctgattgc cgtaggtgct tctttcgagg aaggccagcc cttcatccag
     3481 gctgtcagcg tgaaccaggc tgagcaccgg tccgaagatc tcggtatcgg tgatctcgct
     3541 ggttgcgggt acgtcgtcga ggatcgtcgg cttcacgaag ttgcccgatt cgtatttcgg
     3601 gattctcgtg ttgcgcccgt cgagcagaac tttcgcgccc tgcttttctc ccaccccgat
     3661 gagatgttca acgcgctcct tactctgcga tgtgatcacg gggcccattt gcacgccgtc
     3721 ctccagcccg ttgcccactt tgagtttcga tgccgcatca gcaatcgagt cgcgaaaagt
     3781 cttctgcgct tcaccgatcg tgaccgcaac cgagacagca aggcagcgct ggccggcaca
     3841 gccgaaggcg ctgtcgctga tgatttgggt ggccatcggc atgtcggcgt cgggcagaac
     3901 gatgacgtga tttttcgctc cgccctggca ctggcagcgc ttgccttttt ccgccgagcg
     3961 cgcgtagatg tagcgggcaa cgggagtcga tcccacgaag ctgatggcgc gaactttcgg
     4021 atgatcgatg agcgcgtcga ccacgccttt cccgcca
//
LOCUS       DASPUL010000237         3185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1142263, whole genome shotgun sequence.
ACCESSION   DASPUL010000237 DASPUL010000000
VERSION     DASPUL010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142263"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /gene="cmk"
                     /locus_tag="VEJ00_05990"
     CDS             <1..579
                     /gene="cmk"
                     /locus_tag="VEJ00_05990"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HXX00743.1"
                     /translation="NLESGAMYRALALKAIQKDASFDDEAALVKLAEDSRIELEPTIG
                     GNRTLLDGCDVSMRIRERDVTEAASRVSVHPKVREWMVAHQRALGVGGGVVMEGRDIG
                     TKVFPDADLKIFLDADPVVREQRRMQQQKIHGEVAASVAAELRERDRRDRTRKNSPLI
                     AAPDAVVIDSTSLSEDEVLSQVARLAEQKLNS"
     gene            complement(597..2105)
                     /locus_tag="VEJ00_05995"
     CDS             complement(597..2105)
                     /locus_tag="VEJ00_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXX00744.1"
                     /translation="MSSPVQPGPVQGSPIQTPAPLPPRRRSFAGPFVLIVLGVLCLLG
                     TMRVLSMSRMWHLFANYWPALLIVWGVIKLIEYYQAQREGSRASGIGAGGIFLVIVIM
                     VFGLIATQLEHVNWSGLRDEMNFDDNDFSNIFGENYNFDDHLQQDFPAGAALKVVNTH
                     GAVSVHASDDNKITVSVRKRIGADNQNDADKYNSETKPTITAIGGLVTVDAKVEGAGE
                     HAVETDLDISLPRKAVVSITSRHGDVDLVGREGNIEISSQHGDTSVEDVVGNVKVSQE
                     KGSVKVEQITGDVHVEGRMNEVSVADIKGSAQLDGEFQESVKLARIMKTVTFKSSRTD
                     LELSRMDGSLDLDSDELQAQEIVGPLHLNTRSKNVQLEGVSGDVRLQDDNGTVEVAMR
                     TLGNVQIDNRNGDIQLSVPDKAGFRLDARARDGEIQADFPELKIDSSGQESRATGSVG
                     SGVAHIVLNNEHEGIEIRKAGPQPPKPPEPPTPAKPGKALPAPKDKVEETEN"
     gene            complement(2098..3072)
                     /locus_tag="VEJ00_06000"
     CDS             complement(2098..3072)
                     /locus_tag="VEJ00_06000"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HXX00745.1"
                     /translation="MNCATHSDQAAVAFCRTCGKPLCNNCTRDVRGVIYCEACLAARM
                     EGAPPVAAFAPVQNVSAPVGTPAVAPRPPASGSGPNPAVAGILAGFLPFGVGAVYCSQ
                     YAKGLAHLLIFALLAFGINNGGRWDFLFGFGMAFFWVYQIIDSVRTAKALQEGQPVPD
                     PMGFGQAFSMGEKFDAGKVPTGAVILIVLGVLFLLHTMGVWEFGLDRFWPLIIVALGG
                     WMFFRRWERVKRGCQCGRCRMRGLMGPTMVTLTGVLLLLDTAHAVDLDRTWPAWILAV
                     GVVKLLQSTASSQGHIGPLPSGSVAGAPPQAPPGPTSQPPASGEVSNV"
BASE COUNT          605 a         1003 c          976 g          601 t
ORIGIN      
        1 aatctggaga gcggagccat gtatcgggcg ctggcgctca aggcaatcca gaaagacgct
       61 tccttcgacg acgaggcagc actggtcaag ctggccgagg actcccgcat tgagcttgag
      121 cccaccatcg gtggaaaccg cacgctgctc gacggctgtg acgtgtccat gcgcattcgc
      181 gagcgcgacg tgaccgaagc ggcttcgcgc gtgtcggttc atcccaaggt gcgggagtgg
      241 atggttgctc atcagcgcgc gctgggcgtc ggcggcggcg tggtgatgga aggacgcgat
      301 atcgggacca aagtattccc cgatgccgat ttgaagatct ttctcgacgc cgatccggtt
      361 gtgcgggagc agcgccggat gcagcagcag aaaattcatg gagaagttgc ggcgagcgtc
      421 gcagcggagc tgcgcgagcg tgaccgccgc gaccgcactc ggaagaactc tcccttgatc
      481 gctgccccgg acgccgtggt catagactca acttcattga gcgaagatga agtgctctcg
      541 caggttgcga ggctggccga gcagaaactg aattcttgac tcaatgactc aattctctag
      601 ttctcggttt cttccacttt gtctttcggc gcgggcagtg ccttgccagg cttggccggc
      661 gtcggaggct cgggtggctt cggcggttgt ggtccggcct tgcgaatctc gatcccctca
      721 tgctcgttgt tgagcacgat gtgcgctacc ccgcttccca cgctgcccgt ggctctcgac
      781 tcctgcccgc tgctgtcaat cttcaattcg ggaaagtcgg cctgaatttc gccgtcgcgc
      841 gcgcgcgcgt ccagccggaa tccggctttg tcgggcacgc tcagctggat gtcgccgttg
      901 cggttgtcga tctgcacgtt gcccagagtg cgcatggcga cttcgacggt cccgttgtca
      961 tcctgcaaac ggacatctcc tgagacgccc tcgagctgca cgttctttga ccgcgtattc
     1021 agatgcagcg gccccacgat ttcctgtgcc tgcaattcgt cggagtcgag gtcgagcgaa
     1081 ccgtccatgc gggagagctc caggtccgta cgtgacgatt taaacgtcac ggttttcatg
     1141 atgcgcgcca gcttcacact ctcctgaaac tcgccgtcga gctgggccga gcctttgata
     1201 tcggcaacag agacttcgtt catgcgtcct tcgacgtgca cgtcgccggt gatctgctcg
     1261 accttgaccg agcctttctc ctggctgacc ttgacattgc cgacgacgtc ctccaccgag
     1321 gtgtccccgt gctgggagga aatctcgatg ttgccttcgc gcccgaccag atcgacatcg
     1381 ccgtggcggg aagtaatcga cacaaccgcc tttcgcggca gggaaatatc caggtcggtc
     1441 tcgaccgcat gctctccggc gccttcgacc ttggcgtcga cggtcaccag cccgccaatt
     1501 gcggtgatcg tgggtttggt ttcgctgttg tacttgtcgg catcgttctg gttgtctgct
     1561 ccaatgcgct tgcggacgct gaccgtgatt ttgttgtcgt cggaggcgtg cacgctgacc
     1621 gccccgtgag tgttgacaac cttcagcgcc gctcccgccg ggaagtcctg ctggagatga
     1681 tcgtcgaagt tgtagttttc cccgaaaatg ttggagaagt cgttgtcgtc gaaattcatc
     1741 tcatcgcgca gcccggacca gttgacgtgc tcgagctggg tcgcgatcaa accgaagacc
     1801 atgatcacga tgacgaggaa aatcccccct gcaccaatgc cgctggcgcg gctgccttcc
     1861 cgctgcgcct gatagtactc gatcagcttg atcacgcccc acacgatcag cagcgccggc
     1921 cagtagttgg cgaacaggtg ccacatccgg gacatcgaga gcacacgcat cgtccccagc
     1981 agacagagca cgcccagcac gatcaggacg aacggtccag cgaacgaacg gcgacgaggc
     2041 ggtagcggcg caggagtctg aatcggactg ccttgcacgg gaccgggttg aacggggcta
     2101 gacattggac acctccccgg aagccggagg ctgcgacgtt ggtcccggcg gcgcctgcgg
     2161 cggcgcgcca gcgaccgagc cggacggcaa tggcccgatg tggccctggg acgaggcggt
     2221 gctttgcagc agtttcacca cgcccacggc tagaatccag gccggccagg tacgatccag
     2281 atccacggcg tgcgccgtgt cgagtagaag cagaacaccg gtgagcgtca ccatggtcgg
     2341 gcccatcagt ccgcgcatgc ggcaacgccc gcactggcac ccgcgcttca cgcgctccca
     2401 gcggcgaaag aacatccacc cgcccagagc gacgataatc agcggccaga agcgatcgag
     2461 cccgaactcc cacaccccca tcgtgtgcaa caggaacagc acgccgagca cgatgaggat
     2521 gaccgcgccg gtcggaacct tgcccgcatc gaacttctcc cccatgctga acgcctggcc
     2581 gaagcccatg gggtcgggca caggttgccc ttcctgcagt gctttcgccg tgcgcacgga
     2641 gtcgatgatc tggtaaaccc agaaaaaggc catcccgaac ccaaagagaa agtcccatcg
     2701 tccgccgttg ttgatgccga aggccagcag cgcgaagatc agcaggtgcg ccagtccctt
     2761 ggcatattgc gagcagtaga ccgcgcccac tccaaaggga aggaatccgg caagaattcc
     2821 cgccaccgcc ggattcggac ccgaaccgga agccggtggg cgaggcgcca cggccggagt
     2881 gccgactgga gcagacacat tctgtaccgg cgcaaatgct gccaccggcg gcgcgccttc
     2941 catgcgcgcg gccaggcagg cttcgcagta aatcactccg cgaacatcgc gggtgcagtt
     3001 gttacagagt ggcttgccgc aggtgcggca aaacgccact gcggcttgat cgctgtgcgt
     3061 ggcgcagttc atacgaacct cctgtacgtg gtcaccgaca ctacaggcag atccggattt
     3121 gacggatcga cagacggacc cgcaggaaca ccggccagaa tgacatgatt gtcggtctgg
     3181 ctgta
//
LOCUS       DASPUL010000238         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_181789, whole genome shotgun sequence.
ACCESSION   DASPUL010000238 DASPUL010000000
VERSION     DASPUL010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181789"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VEJ00_06005"
     CDS             <1..641
                     /locus_tag="VEJ00_06005"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HXX00746.1"
                     /translation="GLTVSTLKYCSFAFLLLGLIFTDAETKLLPDKLTLPGFALGIVF
                     SLIVPVNDLASQFLPGMVNLPFSSDVSARLLSLADSLLGAAVGASFIYGAGAIYLRWR
                     GMEGMGFGDVKLMAMVGAFLGTKLTILTIFTASLAGSVFGLTTVLVVWLKRTHRFMRR
                     LANAEAARRRGWQSAQMVYRYYQMPFGVFLGSMAFVAFFFGNQFLRWYGRLW"
     gene            638..1552
                     /locus_tag="VEJ00_06010"
     CDS             638..1552
                     /locus_tag="VEJ00_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00747.1"
                     /translation="MRILANPILLRAAVVFVGATMAFLLSLLCLRLLKKSITEDAEIS
                     SEPSPSLETLPLHLYNTVIQQLKQQKHELQVQSQAEQHRARTQESFSQAVLSNLSCGV
                     LVFGSNGLVKTSNPAAKQILGFGSTTGMGAEDIFRGAVVSSVGSWKAPTVLPDETPTE
                     YGDGSVCLADEVATVLHEGGKQRRVDAEYETPAGDKRYISVTVSPILAADGGGLLGVV
                     CLINDLSELERIRRQQELHGEISAEMALQLRTSLATIAGYAQQLTKNRDPELAEQLAE
                     DIAHEAAHLDQTIGGFLTEKRTAASSSA"
     gene            1679..2347
                     /locus_tag="VEJ00_06015"
     CDS             1679..2347
                     /locus_tag="VEJ00_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00748.1"
                     /translation="MKRIAYIGIGLALTATLALAQDQASSSNSQNAGPSLGDYARHIR
                     KGSAAGKPKVFDNDNLPTEDKLSVIGQAPEPGADNSAQAAPAESAAAPASADNKAQTD
                     NKASAPAQTASTAKTPEEDQAAKQAAWKQWGEKIAAQKEQIDLLTRELNVLQKEYQLR
                     AAAMYADVGNRLRNSGDWDKQDAQYKQQIADKQKQVDDAQQKLTDMQEEARKAGVPSS
                     IAEP"
BASE COUNT          522 a          841 c          764 g          445 t
ORIGIN      
        1 tcggactgac cgtttctacg ctgaaatatt gctcgttcgc ctttctgctg ctcggcctga
       61 tcttcaccga cgctgaaacc aagctcctgc ccgataagct gacgcttccc ggctttgcgc
      121 tggggatcgt tttcagcctc atcgttccag tcaacgatct cgcgtcgcag ttcctgccgg
      181 gcatggtaaa cctgccgttc agcagcgatg tctctgcgcg gcttctctct ctcgccgatt
      241 ccctgctggg cgccgccgtg ggcgcgtcgt tcatctatgg cgcgggggcc atctatctgc
      301 gctggcgtgg aatggaaggc atgggcttcg gcgacgtcaa gctgatggcc atggttggcg
      361 cgttcctggg aacgaaactg accatcctta cgattttcac ggcatcgctg gccgggtctg
      421 tattcggcct gaccacagtt ctcgtggtgt ggctgaagcg gacgcaccgc ttcatgcgcc
      481 gcctggccaa cgccgaagcc gcccgccgcc gcggatggca atccgcgcag atggtctacc
      541 gctactacca gatgccgttc ggcgtcttcc tcggcagcat ggcgttcgtc gcctttttct
      601 tcggcaacca gttcctgcgc tggtacggga ggctctggtg agaatcctgg ccaacccgat
      661 cctgctgcgc gccgcggtcg tgttcgtcgg cgcgaccatg gcgtttctgt tgagcctgct
      721 ctgcctgcgc ctgctcaaga aaagcatcac cgaagatgcc gagatctcat cggagccctc
      781 gccctcgctc gagaccctgc ctttgcatct ctataacacc gtcatccagc agctcaagca
      841 gcagaagcac gaactacagg tgcagtcgca ggcagagcag caccgcgccc gcacccagga
      901 aagcttcagc caggccgtgc tctcgaacct atcctgcggc gttctggtct tcggatcgaa
      961 cgggttggtc aaaaccagca atccggcagc gaagcagatc ctcggcttcg gctcgaccac
     1021 cggcatgggc gcggaagaca ttttccgcgg cgccgtggtc agctccgtcg gttcctggaa
     1081 ggcgccaaca gtacttcccg atgagacgcc caccgaatac ggcgacggct ccgtgtgtct
     1141 cgccgacgaa gtcgcaaccg tgctgcacga aggcggcaaa cagcggcgtg tggacgccga
     1201 atacgaaacc ccggccggtg acaagcgcta catatcggtt acggtctcgc caattctcgc
     1261 cgccgatggc ggcggacttc ttggcgtggt ctgcctgatc aacgatctca gcgaactgga
     1321 gcgcatccgc cgccagcagg agcttcacgg cgagatctcc gcggagatgg cgctgcagct
     1381 gcgcacctcg ctggccacga tcgccggcta cgcccagcaa ctgaccaaga accgcgatcc
     1441 ggagttggcc gagcagctgg ccgaggacat cgcgcacgag gccgcacacc tcgatcagac
     1501 catcggaggt tttctgaccg agaagcgcac ggccgcaagc tcgagtgcat gaagctaagg
     1561 ttgagttcgt tacgggtaag ttaagaacgg agtggaacga gaagcaagtt ttcggcgaag
     1621 gttcggcgta gagcattcgt cgcaccggca aggcagactt tcaacagagg tggaggctat
     1681 gaaacgcatc gcatatatcg gaattgggct ggcactgacg gcaacgctgg cgctggcgca
     1741 agaccaggct tcgtcatcga attcacagaa cgcggggcct tcgctcggcg actacgctcg
     1801 ccacattcgc aagggttcag ccgcgggcaa gccgaaggtc ttcgataacg acaatctgcc
     1861 gaccgaggac aagctctctg tcataggcca ggcgccggaa ccgggcgccg acaactctgc
     1921 gcaagccgcg cccgccgaat cagccgcggc gccggcttcc gccgacaata aggcccagac
     1981 cgacaacaag gcctcggcgc ctgcgcagac cgcgagcacc gcgaaaacgc ccgaagaaga
     2041 tcaggctgcc aagcaggccg catggaagca gtggggcgaa aagatcgccg cgcaaaagga
     2101 acaaatcgac ctgctgaccc gcgagctcaa cgtgctgcaa aaggaatatc agcttcgcgc
     2161 cgccgccatg tacgccgacg tcggcaaccg cctgcgcaac tccggcgact gggacaagca
     2221 ggacgcacag tacaagcagc agatcgccga caagcagaaa caggtcgacg acgcgcagca
     2281 gaaactcacc gatatgcagg aagaggcccg caaggcgggc gttccgtcgt ccatcgctga
     2341 accgtaagaa acccgcgcca ctcaacgcgc catgtaggga cagccgcctc ggctgtccca
     2401 cgagcgcagc gagtctggga gccaaagcat cgcgggcggc tatatccctc ccgaattcat
     2461 caaccccgct gaactgctcc gaaccgcgcc ctcccgcagc cgcgagatct gcctcacgtg
     2521 atgcagcccg tgcaccaggt gaaacttctt ccactgccgg ctattgagcg gg
//
LOCUS       DASPUL010000239         6953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_724842, whole genome shotgun sequence.
ACCESSION   DASPUL010000239 DASPUL010000000
VERSION     DASPUL010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724842"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VEJ00_06020"
     CDS             <1..204
                     /locus_tag="VEJ00_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00749.1"
                     /translation="LSNINEMPDVTKAIGTELRYQYMLTYQPQSPPHDGKWHKISVKL
                     RLPRKFNIFAHIEARPGYYADHQ"
     gene            complement(239..601)
                     /locus_tag="VEJ00_06025"
     CDS             complement(239..601)
                     /locus_tag="VEJ00_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00750.1"
                     /translation="MPTLITQPTRIQAAGNKPKVIDEYIGRVNSKTCAASVAHMRSPE
                     GWIEPGQTPEFDEFTIVLTGMLRVEHKGGILDVAAGQAVIAHAGEWIRYSTPNAGGAE
                     YIAVCLPAFSMEMVHRDQ"
     gene            complement(748..1836)
                     /locus_tag="VEJ00_06030"
     CDS             complement(748..1836)
                     /locus_tag="VEJ00_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00751.1"
                     /translation="MNDRHPCDRSVSSLTTLNPVKITAKHVVAIAAVFLATLACSAQQ
                     SSQADEQSNQSPRKSQPESSNATVTIPAGTNIALVLTNPIQTRYIHHGDDIYAQITSP
                     VNSGDQTVIPPGTFVQGKVDKLIRNGGRGEVRLQSMSITFPDGYVVPIPGPVTLISDD
                     GWALKDPGSGRIVATFALPAAGAGLGALIGHSLGHSQSTTFPGLPPNCGVPTPGCTNG
                     TPGTTLTDNSSRLKSTAIGSMAGLAIGGVAAVAVLFGSHNFYLDVGSPVEMTLPQPVT
                     LQQGEAEEAVQQAEQHPTPVQPVAPRPVPPPPPPGAPVDHGTCWTPGTPGTPPTVIPG
                     PPGPDGIPGPPTIIPGTPPTPGTPYPCP"
     gene            complement(2083..2862)
                     /locus_tag="VEJ00_06035"
     CDS             complement(2083..2862)
                     /locus_tag="VEJ00_06035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX00752.1"
                     /translation="MSTVLITGASGGIGYELAKLFARDRHNLVLVARSADKLGQVAAE
                     LQALGVTVKTIPLDLTDPPAPKFLFDQLQREGTSVEILVNNAGFGAFGEFAQMPEEEI
                     HGQIQLNIAALTELTRLFLAPMVARRSGRIMNVASTAAFQPGPLMAVYYATKAYVLSF
                     SEAIANELRDSGVTVTCFCPGATLTGFAKRAGTEGSRLMKQFGAMSVEKVALDGYRAL
                     MAGRTLAISGAHNWMVAQSTRFSPRKMVTAVSRWVVEKAGS"
     gene            complement(2999..3826)
                     /locus_tag="VEJ00_06040"
     CDS             complement(2999..3826)
                     /locus_tag="VEJ00_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00753.1"
                     /translation="MPRSVLLVLLAALAAPPQKPTFVVPNYHDTTITTRVTRGLMLPM
                     VTTLRLKGSRQRTESHPESAKPFLPFMVHIMQCDQQAGIMLSQHSKTYRLTHWHEPAR
                     ERHARPPKPPDGPEVTVTINSVDTGERRTMGSYQAQHVKTTVTVSPSKEASAKPSRTD
                     IDGWYIDLPGLNCRSEMPDRIPPNVGGWYVTRQIGSHDHLIYKQVGTASRGYTIQETS
                     TERSDGNVVVNKTELVEFSEAPLDDSLFEVPEDYTPAEPPQPRMMERAPVGPGLSPQ"
     gene            complement(3943..5142)
                     /locus_tag="VEJ00_06045"
     CDS             complement(3943..5142)
                     /locus_tag="VEJ00_06045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HXX00754.1"
                     /translation="MPRTLVEHVSSYSEAMLRLTMDLVRIATENPPGNNYGTAIDLLC
                     SALKSLGADEIRVEGHCVLGFVGEGERTLYLSGHYDVVPAQDPSQFQPRVNGKNLFGR
                     GSSDMKSGLAAMLFAAIALRDSGGLRNGRIGLVFVPDEETAGPRGSRYLAKRGILGQH
                     AIGMLTPEPTGGIVWNANRGAITLNVILKGKSAHVGRQYEGINAFDNMLKVAGAVSAL
                     KLEVEQRVTGFHISPKAARRSILLMGGQCSSGANFNIVPERCCFTIDRRINPEEDLET
                     EKKRLVAVLEGFKKTSQDLEIEVLQEGHSSSTAEDHPLGRALARSIRSVAGKAPRFEM
                     CGGLLETRFYAEGGIPAYAYGPGLLSVSHGPKEFVPIDRITECARIYALTAAEMFGKQ
                     SRRKITR"
     gene            complement(5286..6290)
                     /locus_tag="VEJ00_06050"
     CDS             complement(5286..6290)
                     /locus_tag="VEJ00_06050"
                     /inference="COORDINATES: protein motif:HMM:NF009498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HXX00755.1"
                     /translation="MKLTPGKLAGLKKVSNDRGVIAAAAMDQRGSLQKSLSKEKGGGE
                     VTDAMMEEFKSLVSEVLTPHASAILLDPEWGLPASKRRAKNAGLLLAYEKTGYDKTGP
                     GRLPDLLDHWSARRLKEAGADCVKILLYYTPDDPKNVNETKHAWVERIGDECRANDIP
                     FFLEFVGYEEGADEKGLEYAKKKPHIVTESMREFSKDRYGVDVLKVEVPVNMKFVDGT
                     RCFAGTKAYTKKEAIDLFHKAAGVATKPFIYLSAGVSNAEFSETLELAGESGVKFNGV
                     LCGRATWKDGIPVYAKQGAAAFRRWLETEGVKNINNVNDKLKAATSWHPIYGVELVHA
                     "
     gene            6519..6680
                     /locus_tag="VEJ00_06055"
     CDS             6519..6680
                     /locus_tag="VEJ00_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00756.1"
                     /translation="MLLVLMVCCAVGAVFLIGLGAFTEFRELWTHPDHRVLAGGSRRS
                     DERRDGLRR"
BASE COUNT         1308 a         2053 c         2127 g         1465 t
ORIGIN      
        1 ctcagcaaca ttaacgagat gcctgacgtc acgaaggcga ttggaactga gcttcgttat
       61 caatacatgc tcacctacca gccgcagtct ccgccccatg acgggaaatg gcacaagatc
      121 agcgtgaagc tgcggctgcc ccgcaaattc aacatctttg cccacatcga agcccggccg
      181 ggctactacg ctgaccacca ataacacgag cacgcggaaa gaactcaccg ggctcggctt
      241 actggtcgcg gtgaaccatc tccatggaaa acgcgggcag gcagacggcg atatactcgg
      301 ctccgcctgc gtttggcgta gaataccgaa tccactcccc ggcgtgcgca atgactgcct
      361 gtccggctgc cacatcgagg atgcctcctt tgtgctccac ccgaagcatt cctgttagca
      421 caatcgtgaa ttcgtcgaac tcgggagtct ggccaggctc gatccatccc tccgggctgc
      481 gcatgtgggc gacgctggcc gcgcaggtct tggagttgac gcggccaatg tactcgtcaa
      541 ttactttggg cttgttgccc gcagcctgga tgcgtgtcgg ttgggttatc agtgtgggca
      601 tggttcgggc ttctggctgc gggcatccac tttccgccac gcccgagccg cacacaagag
      661 tacaacaaag aaatcggggc cgagatcagg gagtggattc cgggtctttg gtccctgacc
      721 cctggcccct agcccctgat cgcgtcccta cgggcaggga tacggtgtcc ccggcgttgg
      781 cggcgtaccg ggaatgatcg tcggtggccc cggaatgccg tctggtccgg gcggtcctgg
      841 aatcacggtc ggcggcgtac caggagtccc gggtgtccag caggtgccgt gatctaccgg
      901 cgcgcccgga ggtggaggcg gcggcaccgg ccggggcgcg actggctgca ccggcgtggg
      961 atgctgctca gcctgttgta ccgcctcttc agcctcgcct tgctgcaacg tgaccggctg
     1021 cggcagggtc atttctactg gcgagcccac gtccagatag aaattgtgcg agccaaacag
     1081 gacagcaacc gcagctacgc cgccaatcgc cagacctgcc atgctaccga ttgccgtact
     1141 cttcaggcgg ctggagttgt cagtaagggt cgtgccagga gtcccattcg tgcaaccggg
     1201 ggtcgggacg ccacagttcg ggggcagacc cggaaatgtt gtgctctggg aatgaccaag
     1261 ggagtggcca atcagcgcgc ccaagccggc gcccgcagcc ggcagcgcaa atgtggcaac
     1321 gatgcggcct gaacctgggt ccttcagcgc ccagccgtcg tcactaatca gcgtcaccgg
     1381 tccgggaatg ggaacgacat acccgtcggg aaaggtgatc gacatcgact gcagacgcac
     1441 ttcgccgcgt ccaccgtttc ttatgagctt gtcaactttt ccctgtacga aagttcccgg
     1501 cgggatcacg gtttgatcac ccgaatttac cggagaggtg atctgcgcat aaatgtcgtc
     1561 tccgtgatga atgtaccggg tctgaatggg gttcgtgagc acgagcgcga tgttggtgcc
     1621 cgccggaatc gtcaccgtgg cattgctcga ctctggctgc gacttgcgtg gcgattgatt
     1681 ggactgttca tcagcttgcg acgactgctg tgctgagcaa gccagcgttg ccagaaaaac
     1741 ggcagcgatg gcaacaacgt gcttcgctgt gatcttcacc ggatttaatg ttgtaaggga
     1801 cgaaacgctt cgatcgcagg ggtgtcggtc gttcattctg cacctcctca cgaaagctgt
     1861 tgcgtggcgc tttcggtttc gacggggcct aacggagata accctcgttc agtaatttgg
     1921 actcagcgct gtgtgggatg ggtatccgtc aagcaggtaa agctttgcaa aagaacgtag
     1981 gacttttgta aatcccgtgc tggggacggg atttgatgag atttgaatca ttcacttgag
     2041 gcgaaactta ggtcaccatc acgagacttg ccggcacgcc tgctacgagc cggctttttc
     2101 gacgacccag cgtgagacgg ctgtgaccat cttgcgcggg gagaagcggg tggactgcgc
     2161 caccatccag ttatgcgcgc cggaaatagc cagcgtgcgt ccggccatca gcgcgcgata
     2221 gccgtcgagc gcgacctttt ccacgctcat ggctccgaat tgcttcatca ggcgcgatcc
     2281 ctcggtgccg gcacgtttgg cgaagccggt aagtgttgct ccgggacaga agcaggttac
     2341 ggttacgccc gagtcccgga gttcgttggc gattgcttcg gagaaggaaa gcacatatgc
     2401 cttagtcgcg taatagacgg ccatcaacgg tccgggttga aaggccgcgg tggaggcgac
     2461 attcatgatg cgccccgagc gccgggcaac catgggagcc aggaaaaggc gcgtgagttc
     2521 ggtgagagca gcgatgttca gttgaatctg cccatggatt tcttcctccg gcatttgggc
     2581 gaattcgccg aacgccccga accctgcgtt gttgacgagg atctcgacgc ttgtgccttc
     2641 gcgctgcagt tgatcaaaga gaaacttcgg cgctggcggg tcagtgaggt cgagagggat
     2701 ggttttgacc gtaaccccga gagcttgcag ttcggcggca acttgcccca gtttgtcggc
     2761 gctgcgggcc accagcacaa ggttgtggcg atcgcgagcg aaaagcttcg ccagttcgta
     2821 cccgatgccg cccgaggcgc cagtgatgag cacggttgac atgcgcctat tctacgggac
     2881 gtgaagggcg cggaagcctt cgtccggagc aactcatcgg attgttcgcc ctggctgccg
     2941 gcttcatggt gaagcccagg gattccgcag cctgaagtgc cagtcgcggg gcaacaggtt
     3001 attgcggtga caagccgggc cccactggtg cccgctccat catgcgaggc tgtggtggtt
     3061 cggccggagt gtaatcctcg ggaacctcaa acagggaatc gtccagcggc gcctcagaaa
     3121 actcgaccaa ttctgtcttg ttgaccacca cattgccgtc cgaccgttcg gtggacgttt
     3181 cctggatggt atagccacgc gaagcggtgc ccacctgttt gtagatcagg tggtcatggc
     3241 tgccaatctg tcgtgtgacg taccaccctc ccacgttcgg cggaatccgg tccggcattt
     3301 ccgagcggca gttcaggccg ggaaggtcga tataccagcc gtcgatgtcg gtccggcttg
     3361 gtttcgcgga ggcctccttg ctcgggctta cggtaaccgt ggtcttgaca tgttgcgcct
     3421 ggtagctacc catggtgcgc cgttcacctg tgtccaccga gttgatcgta accgtgactt
     3481 ccggcccgtc gggtggcttc gggggcctgg cgtgacgttc ccgcgccggt tcatgccagt
     3541 gagtgagtcg atacgttttc gaatgctgag acaacatgat tcctgcttgc tggtcgcact
     3601 gcatgatgtg gaccatgaag ggcaggaacg gcttcgccga ttccggatgc gactcagtcc
     3661 gctgccgcga tcccttgaga cgtaacgtgg tcaccatggg aagcatgagc ccgcgcgtga
     3721 ctcgcgtcgt gatcgtcgtg tcgtggtagt tgggaaccac gaaggtgggt ttttgcggcg
     3781 gggctgccag agcggcgagc aagaccagga gtacggagcg cggcatagtt cctctctttg
     3841 catcaggtga gcggcggcct acgtcttatt tggcttcgat caaggaacgt agattccggg
     3901 ttgccttgct ccttcaattg aatccatgaa caggggagaa agttaccggg tgatcttccg
     3961 ccgactttgc tttccaaaca tctcagccgc ggtgagagca tagattctgg cgcattcggt
     4021 aatgcggtcg atgggtacaa actccttggg accatgggaa acgctgagca ggcccggtcc
     4081 ataggcgtaa gccggaattc cgccctcggc gtagaaacga gtctccagca acccgccgca
     4141 catctcaaag cgcggcgcct tgcccgcgac cgagcggatg ctcctggcca acgcccggcc
     4201 cagcgggtga tcttccgctg tgctgctgga gtgcccttcc tgcaaaactt caatctccag
     4261 atcttggctg gtcttcttaa agccttccag gaccgccacg aggcgcttct tttccgtttc
     4321 caagtcctct tccgggttaa tgcgccggtc gatggtgaag cagcagcgct ccggaacgat
     4381 attgaaattg gcgccgctgc tgcactgccc gcccatcagc agaatggacc gccgcgccgc
     4441 cttcggcgag atgtggaatc cagtcacgcg ctgctcgacc tccagcttca gcgcgctcac
     4501 cgcgcctgca actttgagca tgttgtcgaa ggcgttgatt ccttcatatt ggcggcctac
     4561 atgcgcgctc ttgcccttga ggatcacgtt cagggtaatg gcgccccggt tcgcgttcca
     4621 cacgatccca ccggttggct ccggtgtgag catcccgatg gcgtgctgac ccaggatgcc
     4681 gcgcttagca agatagcgcg agccgcgcgg acccgccgtt tcctcgtccg gcacgaatac
     4741 gagaccgatt cggccgttgc gcaggccccc gctatcgcgc agcgcgattg cagcgaacag
     4801 catcgccgcc aggccgctct tcatgtccga agaaccgcgg ccaaacaagt tcttaccgtt
     4861 cacacggggc tggaattgcg acggatcctg tgcgggcacg acgtcgtagt ggccgctgag
     4921 gtacaacgta cgttcgcctt cccccacaaa cccgagcacg cagtgaccct ccacgcggat
     4981 ctcgtccgcg cccagacttt tcagggcgct gcacagcaag tcgattgccg tcccatagtt
     5041 attcccgggg ggattttcgg tcgcgatgcg caccaggtcc atcgtcaagc gcagcatggc
     5101 ctcggagtaa gaggagacgt gttcaaccag tgtgcgaggc attagttctc tgtctcagaa
     5161 tcgatggggt tcgcacaggg gcggagacac cggttcgagg atagcagcaa gtgggacgag
     5221 acaagaaggg cgcgacgatt tgccgcgccc gcaaactgcg ggcacatctg cccgctccac
     5281 acagcttacg cgtgcaccaa ctccacgccg taaatcgggt gccacgacgt cgctgctttc
     5341 agcttgtcgt tgacgttgtt gatgttcttc accccttcag tttcaagcca cctgcggaag
     5401 gcggccgctc cctgcttcgc gtagaccgga atgccgtctt tccacgtcgc gcgtccacaa
     5461 agcacgccat tgaacttcac tccagattct cccgccagtt cgagcgtctc gctgaactcg
     5521 gcattgctga caccagcgga aagataaatg aacggtttgg tcgccactcc ggccgccttg
     5581 tggaacaggt cgatggcttc tttcttggta taggccttag ttccagcaaa gcagcgagtg
     5641 ccatccacga acttcatgtt tacggggact tcgactttca gcacgtcgac gccgtagcgg
     5701 tctttcgaga actcccgcat gctctcggtg acgatatggg gtttcttctt ggcgtactcc
     5761 aggcccttct catcggcccc ctcttcgtag ccgacgaatt cgaggaagaa cggaatgtcg
     5821 ttggcacggc actcgtcgcc gatgcgctcc acccaggcgt gcttggtttc gttgacgttc
     5881 ttgggatcgt cgggcgtgta gtagagcagg atcttcacgc aatcggctcc cgcttctttc
     5941 agtcgacggg ccgaccagtg atccagcaga tcgggcaaac gcccgggacc ggtcttgtcg
     6001 tatccggttt tttcataggc gagcagcagg ccggcgtttt tcgcgcggcg ctttgaagcc
     6061 ggcagtcccc actcggggtc aagcaggatg gccgaggcat gcggcgtcag aacttcgctg
     6121 accagcgatt tgaactcttc catcatggcg tcggtcacct cgccgccgcc tttttccttg
     6181 gacagcgact tctgcagcga gccgcgctga tccatggcag cggcggcgat gacgccgcga
     6241 tcgttggaga ctttcttgag gccggcaagt ttccccgggg tgagcttcat ggaatcctcc
     6301 tgaatgcgac agagatgatt gcttgagaga cacacaattg taaaccaagg ctgcggggga
     6361 agccgcggtt gctctttgaa ctcgggaggg caggtgccgc ttcgggactg gagtctttca
     6421 tcacaggggc gtgagcgccg tcacagcgga acgtcggtgt gctggctaaa ctcaaaccga
     6481 acgtcacgcc aaagaacgac aggcatgggg aggcgaatat gctgctcgtt ctcatggttt
     6541 gctgcgctgt cggagccgtc ttcctgatcg gtcttggcgc attcaccgaa ttccgggaat
     6601 tgtggacgca ccccgatcac cgagtgctgg ccggcggttc acgccgaagc gacgagcgcc
     6661 gagacggttt gcgtcgctga gttgaaatgg cgccgacacc tcgcaaggcc gcattccagt
     6721 cttcgtccgc gtgcagggct ttgagatggg aatcgggcgt gctgcccgta acgcccggaa
     6781 agcccagggc tttcgcctaa agaggaggtc caaatgagga aatccctatg cgttgttgtc
     6841 atgttggctg ccggctgtct tttggcgcag gatttgaaac cgattctttt gccgcagccg
     6901 cagaccgacg gaggccggcc gctgatgcag gcgctcaaag aacgcaagac aac
//
LOCUS       DASPUL010000240         4589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_724880, whole genome shotgun sequence.
ACCESSION   DASPUL010000240 DASPUL010000000
VERSION     DASPUL010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724880"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            538..1521
                     /locus_tag="VEJ00_06060"
     CDS             538..1521
                     /locus_tag="VEJ00_06060"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXX00757.1"
                     /translation="MDFADQKESGGSSARPRMSAFDDTEIFRSILEDLPTGVCVIDLQ
                     KRIVLWSDGAERITGRPRYEVIGHCCIGEPMLHCDQPGCEWCKEDCPFARAMKTSHEA
                     EGLGFLHHKAGHQVPVHVRAVPVHDEHGSIVGAAAIFEDRLTEIGSDRREDGRQFPGC
                     VDDVTEVASHAMMQSHLRESLATFAEVHVPFGILCMRLEGLDHFRASFGPEAASSLLR
                     MVARTLESAVWRTDFVGRWSDDQFLIILNGCRDDALRSVRERLHRMLFGEGIEWWGEK
                     RSLPLVMGQATAQLEDSVESLVERAQKSLEIASAGRAQGAADSGGASQSAG"
     gene            1550..2425
                     /gene="motA"
                     /locus_tag="VEJ00_06065"
     CDS             1550..2425
                     /gene="motA"
                     /locus_tag="VEJ00_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HXX00758.1"
                     /translation="MFAIIGIVVVFGCIVAGYLMEHGNLRVLIQPAELVIIGGAAVGT
                     LLVANPLHIIKKIAAGIGGVFGSSRFGKQTYIDTLKMMYDLLNKARKDGLMALEGDVE
                     EPEKSQVFTKNPAFLKNHHARDFVCDSLRMAITGIDAFELDQMLDLDLEVHHHDATQS
                     TSALSTVADSLPGLGIVAAVLGVVITMGALGGPPEEIGHKVAAALVGTFLGILLCYGL
                     IGPIAANMTKASEEEHAFLYVMRVLMISFLKGSAPIMAVEVARRAVPGHVRPSFKELE
                     AACRGGGAAAAAASA"
     gene            2432..3253
                     /locus_tag="VEJ00_06070"
     CDS             2432..3253
                     /locus_tag="VEJ00_06070"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXX00759.1"
                     /translation="MDKTRPIIIVKKKGSHGGHHGGAWKVAYADFVTAMMALFIVLWL
                     LNTSKQIQEAVGGYFKDPTGTSKKVGTNMQGVGENFVLTRDNMPKLKDQLQQAIRQMT
                     DFEKLKNHIEMTVTAEGLRIELSESATGTFFDSGSAKLNADGRELLVTLAQELSKLPN
                     KISIEGHTDSNPYAASANYGNWELSADRANAARRVMQANGLRADQVMQVRGFADQRLR
                     KPEAPLDPANRRISLIVQYIVKDPADEEAKSTTGDDPKKAETESATPAEKPAKKE"
     gene            3336..>4589
                     /locus_tag="VEJ00_06075"
     CDS             3336..>4589
                     /locus_tag="VEJ00_06075"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXX00760.1"
                     /translation="MRRFARLACTLVLTFTFVPGTWSQEKVDLETISRIRYEGFHNSK
                     VMELASGLMDSIGERLTGSPNVKRANEWTRDQLTAMGLSNAHLEAWGPFGRGWANQYV
                     NVRMTAPDIVPLIAYAKAWTPGTNGVLTGKCVRATIESQKDFDKYRAKLAGMIVILGA
                     DAEVKPISEAPFKRYSDDDLAKEGEYQGAWEHPPSTLAAFLKRQQFSKDINQFYADEK
                     VLVVIDHSRGTSGGGTVFVQSGGSYKTGETATVPQLTMASEQWNRIARLLDQNKEVTL
                     ELNVINNFYDDDPMQYDTIAEIPGTDKKDEIVMLGAHLDSWHSGTGATDNGAGTVVMM
                     EAVRILKALDIKPRRTIRIGLWTGEEQGLLGSQRYVEKHFGSRPPIDTPFMKDQPTLL
                     RHEAGPVTVKAEQAKVSAYFNVDNGT"
BASE COUNT         1002 a         1324 c         1397 g          866 t
ORIGIN      
        1 gctcatcttc gggcaggctg tcgagaatct cgcggacgga gcaggttccc agcttccagt
       61 actgctccag gattcgcgtc tcggcttggg agagatgctt ctttttcata tctactacgc
      121 agtacggaaa ttctactaca ctctacgttg gtcgagcaac gatacgactg aaaagtctca
      181 ggcaacattc gctgatgagg ttaccgcgtg acgccacagt ctgtccggcg gtgagaattg
      241 agtgtccgtt attgggcagc gggtacgcaa gcgcaatggc gacacctgac ccgcaaggag
      301 cgaaaggaaa aaacaggcaa aaataacgag cgtcctgaga ctcttgcgag tccaggacgc
      361 ccgggcagcc ctcccccaga actgctcacc gatgaaggta aactcggaga gtccttctgc
      421 ctatggcact tcggtgccat gaggggcccg aaagtgccag ttctctatcc ccctgcaaat
      481 taagggtttg ccgccatccg cgagatcccg cgctgggcgc aggatctgaa gtcgactgtg
      541 gatttcgccg atcagaaaga gtcaggcggc tcttccgccc ggccacgcat gtccgcattc
      601 gacgatactg agatctttcg cagcatcctg gaggacctgc cgactggcgt ctgcgtcatc
      661 gacctgcaga agcgaatcgt gttgtggagc gacggcgccg aacgcattac cggccgtccg
      721 cgctacgaag tgattgggca ttgctgcatt ggcgaaccca tgctgcactg cgatcaaccc
      781 ggctgcgagt ggtgcaagga agattgtccg ttcgcccggg ctatgaagac gtcgcacgag
      841 gcagagggcc tcggctttct ccaccacaag gccgggcacc aggtgcccgt tcacgtgcgg
      901 gcggttccgg tgcacgatga gcacgggtcg atcgttggtg cggccgcaat cttcgaggac
      961 cggctgacgg agatcggctc ggaccgccgc gaagacgggc ggcagtttcc gggctgtgtc
     1021 gacgacgtca ccgaggttgc cagccacgcc atgatgcagt cgcacttgcg cgagagcctg
     1081 gcgacattcg ccgaggtcca tgtgcccttc ggaatactct gcatgcgcct cgaagggctc
     1141 gaccatttcc gcgcgagctt cgggccggaa gccgcctcgt cgctgcttcg catggtggcg
     1201 cgcacgctgg agagcgcggt gtggagaacg gatttcgtcg gacgctggag cgacgatcaa
     1261 ttcctgatca ttctcaacgg gtgccgcgac gatgccttgc gctcggtacg cgaaaggttg
     1321 caccgcatgc tgttcgggga agggattgag tggtgggggg aaaagcggtc actgccgctt
     1381 gtgatgggcc aggcgacagc gcagctcgaa gattcggtcg aatcgctggt agaacgagcg
     1441 cagaagtcgc tcgaaattgc ttcggccggc cgggctcagg gtgctgcgga ttcgggagga
     1501 gcctcacagt cagcgggata gttcggaagc gggacttgga gagcagggaa tgttcgcgat
     1561 cattggcatc gtcgtcgtct tcggttgcat tgtggccggc tacttgatgg aacacggcaa
     1621 tctgcgcgta ctcatacaac ccgccgaact ggtcatcatc ggcggcgccg ccgtgggcac
     1681 gcttcttgtc gcgaacccgc tgcacatcat caagaagatt gctgccggaa tcggcggcgt
     1741 cttcggcagt tccaggttcg gaaagcagac ctacatcgac acgctgaaaa tgatgtacga
     1801 cctgctgaac aaagcgcgca aagacggtct catggcactc gaaggcgatg ttgaagaacc
     1861 cgagaagagc caggtgttca cgaagaatcc tgctttcctc aagaatcacc atgcccgcga
     1921 ctttgtctgc gattcgctgc gcatggccat caccgggatc gatgccttcg aactcgacca
     1981 gatgctcgat cttgacctcg aagttcatca tcacgatgcg acgcaaagca cgtccgctct
     2041 cagcaccgtg gccgattccc tgcccgggct ggggattgtt gcggccgtgc tgggcgtcgt
     2101 catcaccatg ggagctctcg gcggcccgcc ggaagagatc gggcacaagg tggccgccgc
     2161 gctggtggga acgttcctgg gcatcttgct ctgttacgga ctgattggcc cgatcgcggc
     2221 caatatgacc aaagcctccg aggaagagca tgcattcctc tacgtgatgc gagtgctgat
     2281 gatctccttc ctgaagggca gcgcgccgat tatggcggtg gaagtggcgc ggcgcgcggt
     2341 cccgggtcac gtgcggccgt cattcaagga gctcgaagcg gcctgccgtg ggggaggagc
     2401 ggcggcggca gccgcgtctg cctaacgcaa aatggacaag acacgcccca tcatcatcgt
     2461 gaagaaaaaa ggcagtcacg gcggccatca cggtggcgcg tggaaagtag cgtatgccga
     2521 ctttgtcacc gccatgatgg cgctgttcat cgtgctgtgg ctgctcaata ccagtaagca
     2581 gatccaggag gccgtcggag gatacttcaa ggatcccacg ggcacttcaa aaaaagttgg
     2641 gaccaacatg caaggcgtgg gggagaattt cgttcttacc cgcgacaaca tgcccaagct
     2701 gaaagatcag ctgcagcagg ctatccggca gatgaccgac ttcgagaaac tgaaaaacca
     2761 catcgaaatg acggtcactg cggaaggcct tcgcattgaa ctctcggagt ctgctacggg
     2821 tacgttcttt gatagcggca gcgcaaaact aaatgccgac ggacgcgaac tgctggtcac
     2881 cctcgcgcag gagttgagca aactacctaa caagatttcg attgaagggc acaccgactc
     2941 gaatccctac gccgcgtcgg cgaactatgg caattgggag ctctcggccg atcgcgccaa
     3001 cgccgcccgc cgcgtgatgc aagcgaatgg gctaagagcc gatcaggtca tgcaggtgcg
     3061 gggcttcgcc gatcagcgcc tgcgtaaacc cgaggcgcca ctggatccgg ccaaccgtcg
     3121 catctcgctg atcgtgcagt acatcgtgaa ggaccccgcc gacgaagaag ccaagtcaac
     3181 gaccggcgac gacccaaaaa aggcggagac tgagtccgca acccctgccg agaaacccgc
     3241 gaaaaaagag tagaccctgc tgctcgaccc aaacccatag cgttgttcca gttatataat
     3301 cggcgctttg atccttgcac tgaggagcgc tcttgatgcg tcgctttgca cggctggcct
     3361 gcactctcgt cctaacgttc acatttgttc ccggcacatg gtctcaggaa aaagttgatc
     3421 tggaaacgat ctcacgcatt cgctacgagg gctttcacaa ttccaaagtg atggaactcg
     3481 cttcaggcct catggattcg atcggcgagc gcctcacggg ttctcccaac gtgaagcggg
     3541 cgaacgagtg gacgcgggac cagctaaccg cgatggggtt gagcaatgcc cacctcgaag
     3601 cctggggccc ttttggccgc ggatgggcca accagtacgt taacgtgcgc atgaccgcgc
     3661 ccgacatcgt ccctctaatc gcttatgcca aagcgtggac gccgggcacg aacggtgttc
     3721 tgacaggaaa gtgtgtacgc gcgaccatcg agtctcagaa agacttcgac aagtaccgcg
     3781 ccaaactggc gggcatgatc gtgattctag gcgctgacgc cgaggtgaaa ccgatcagcg
     3841 aagctccttt caagcgctac agcgacgatg atctggccaa ggagggcgaa tatcaaggtg
     3901 cgtgggaaca tccaccctcg actttagcgg cttttctcaa gcgccagcag ttcagcaagg
     3961 acatcaacca gttttatgcc gacgagaaag tgctggtggt cattgatcac agtcgcggca
     4021 ccagtggcgg cggcactgtg ttcgtgcaat ctggcggctc gtacaaaacc ggggaaaccg
     4081 cgacggtccc gcagctcacc atggcgtcgg agcagtggaa ccgcatcgcc cgcctgctcg
     4141 atcagaacaa ggaggtgacg ctcgaattga acgtgatcaa caatttctac gacgatgatc
     4201 cgatgcagta tgacacgatc gcggagattc ccggcactga caagaaagat gagatcgtga
     4261 tgctgggcgc gcatcttgac tcgtggcact ccggaactgg agctaccgac aacggagcgg
     4321 gcacggtcgt catgatggag gccgtgcgca tcctgaaagc gctcgatatc aagccccgcc
     4381 gcaccattcg gatcggatta tggacgggcg aggaacaggg tctgctcggc tctcagcggt
     4441 acgtcgagaa acactttggc tcgcgtccgc cgatcgacac tcccttcatg aaagaccagc
     4501 ccactctgtt gcgccatgaa gccgggccgg tcacggtgaa agccgagcag gccaaggtct
     4561 cagcctattt caacgtggac aacggtaca
//
LOCUS       DASPUL010000241         3091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_725336, whole genome shotgun sequence.
ACCESSION   DASPUL010000241 DASPUL010000000
VERSION     DASPUL010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725336"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..494)
                     /locus_tag="VEJ00_06080"
     CDS             complement(222..494)
                     /locus_tag="VEJ00_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00761.1"
                     /translation="MHDRTRSLDQTLPYIGELIKHGQITVGNVRPVGCVAVAHDGRQT
                     VAMLLRREGETATELLTRLDMAIAKAIAEGVRVDEVNPVNQQSFPK"
     gene            complement(470..2026)
                     /locus_tag="VEJ00_06085"
     CDS             complement(470..2026)
                     /locus_tag="VEJ00_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006482547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HXX00762.1"
                     /translation="MSELSPDALRALLLTQHEQLVSTQEQLQSREREIEHLKLLLAKL
                     HRMQFGRKSEKLTRQIEQLELRLEELQADCGERPIQADPAPTTVGAHFPAKPSRRALP
                     DHLPRETRTYMPKEVVCPDCQGELRKLSEDVSEVLEYVPEQIKVIRYVRPKLSCRKCE
                     RIVQAPAPSRPIARGLAGPGLLAHVLVSKYADHLPLYRQCEIYERQGVELERSTLADW
                     VGGSSGLLDPLVEALRRYVMAAGKLHADDTRVPVLAPGTGKTKTGRLWTYVRDNRPAG
                     DEAAPAVWFAYSADRGGEHPVQHLRTFQGALQADAYAGFNQLYEDGRIQEVACWAHVR
                     RKFYDLEQAHASPLAREAVERIAALYAIEDEVRGRPPDERRQVRQTRARPLLQSLHEW
                     FEVSLTKLSRKSDTTAAIRYALTLWTALTRYCDDGRLEIDNNAAERALRAVTLGRKNY
                     LFAGSDAGGERAAVIYSLIGTAKLNGLDPESYLRDVLTRIADHPICRIEELLPWNFAP
                     NRPCTIEPAA"
     gene            complement(2126..2473)
                     /gene="tnpB"
                     /locus_tag="VEJ00_06090"
     CDS             complement(2126..2473)
                     /gene="tnpB"
                     /locus_tag="VEJ00_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HXX00763.1"
                     /translation="MITLPSNTQIWIAAGVTDLRRGFMGLSALVQSKLEQSPMSGQVF
                     VFRGRRGDLIKLLWFDGDGLCLFAKRLERGRFVWPQATSGSVSLTRAQLSMLLEGIDW
                     RRPERTWQPQLSV"
BASE COUNT          638 a          988 c          857 g          608 t
ORIGIN      
        1 agcgtaaccg gcggatgata tcagcaagac tccggacgag ttgaacctcg gccttggtgc
       61 aggaagatgc cgatttgagc gatattcggc tgggtcggaa aaggtggtag ccccagcgat
      121 ttgcgctgag gcggtcgctg agtaacggct cgtcggaggc cgcattgaca acgcgttgga
      181 gttgtgaagt gcccagctaa aatagaatcg gaagtggact tttatttggg gaaggattgc
      241 tggttgaccg ggttgacctc gtcgacgcgg acgccttcag cgatggcttt agcgatcgcc
      301 atgtcgagac gagtgagcag ctcagtcgca gtctctccct cacggcgaag caacatagct
      361 acagtctgac ggccatcgtg agcaaccgcc acacagccga ccgggcgcac attgccgacg
      421 gtgatctggc catgctttat gagctccccg atgtaaggca gggtctggtc taggctgcgg
      481 gttcgatcgt gcacggtcgg tttggtgcga agttccacgg cagcagctcc tcgattcgac
      541 atatggggtg atcggctatg cgcgtgagca cgtcacgtag atagctttct ggatcgagcc
      601 catttagttt cgccgtgccg atcagactgt agatgacggc cgcccgctcc ccgcctgcat
      661 cggaaccggc aaaaagataa ttcttccgtc ccagagtaac ggcacgcaaa gcacgttccg
      721 cggcgttgtt gtcaatctcc aagcgaccat cgtcgcagta ccgggtcaac gccgtccaca
      781 acgtcaacgc atagcggatc gccgccgtcg tatccgactt acgagaaagt ttcgtcagtg
      841 agacttcgaa ccactcgtgt aacgactgga gcaagggacg agcgcgggtt tggcggacct
      901 gtcgtcgttc atccggtggc cgtccgcgaa cctcatcttc gatggcatac aaagcggcga
      961 tccgttccac agcttcccgg gctaagggcg aagcgtgagc ctgttccaga tcgtagaact
     1021 tgcgccgaac gtgagcccaa cacgccactt cctgaatccg accatcttca tacagttgat
     1081 tgaagccggc gtaagcatcg gcctgcagcg caccctgaaa cgtccgcaga tgttgcacgg
     1141 ggtgttcccc tccgcgatcg gcagaatacg caaaccacac cgcgggagcg gcttcgtcgc
     1201 ccgccggccg attgtcgcgc acgtaagtcc acaaacgccc cgtcttggtc ttgcctgtac
     1261 ccggcgccag caccggcacc cgcgtgtcgt cggcatgcag cttgcctgcc gccatcacat
     1321 aacgccgcag cgcttccacc agcggatcca acaacccact gcttccaccc acccagtccg
     1381 ccagagtcga tctttctagt tcgactcctt gccgctcata gatctcgcat tgccgataca
     1441 ggggaagatg gtccgcatac ttggagacca gcacatgcgc cagcagaccc ggtccagcca
     1501 gcccccgtgc gattggccgg cttggggccg gagcttgcac aatccgttcg catttccggc
     1561 agctcagctt cgggcgtaca taacgaatca ccttgatctg ctcgggcacg tactccagca
     1621 cttccgacac atcttcgctt agcttgcgca actcaccctg gcaatccggg cacactacct
     1681 ctttgggcat gtaggtgcgc gtttcgcgcg gaagatgatc gggcaaggct cgccggctgg
     1741 gtttcgctgg gaaatgcgcg ccaaccgtcg taggcgctgg atccgcctgg atcgggcgct
     1801 cgccgcagtc ggcttgtaac tcctctaacc gcaactccag ttgttcaatc tggcgcgtca
     1861 acttctctga cttacgtcca aactgcatgc ggtgcagctt agccagcagc agtttcagat
     1921 gctcgatctc ccgttcgcgc gactgtaact gctcctgcgt ggagactagc tgttcatgct
     1981 gcgtgagaag caacgctcgc agagcatccg gggaaagctc gctcagatcg ggaagtgcgg
     2041 gcacaaccat gcgcggagta tcgcatgaat gcccgctttt cgatcggccg tacctgcaaa
     2101 aaagttgtgg aaagaacctc gcgggttaca ccgacaactg cggttgccag gtgcgctccg
     2161 gccgccgcca atcgatgcct tccaacaaca tcgagagctg cgctcgcgtc aacgaaacgc
     2221 tgccactggt cgcctgcggc cacacgaacc gtccacgctc cagccgcttc gcaaacaggc
     2281 atagcccgtc gccatcgaac cacagaagtt tgatcaaatc acctcgtcgt ccgcgaaaca
     2341 caaaaacctg tcccgacatc gggctttgtt ccaacttgct ttgtaccagg gcgctcagac
     2401 ccatgaagcc gcgccgcaaa tccgtcaccc cggcggcgat ccatatctgc gtgttgctgg
     2461 gtagagtgat catcgctgca ggcactccag caacacccgc aagacaaccg gatccgcgct
     2521 gccttcgatg cgtacttgcg catgccgcag ttgcaggtga atcgtacccg acgaggaaat
     2581 ctgcaccgct cgggattcac acaagggccc tggcaacggc gtagaactct ccgcggtgac
     2641 tcgcaccggc agcagtttgg cctcactgcc ctcacccagt agccccgcac gatacttccg
     2701 acaccagttg aagactaagt tcgcattgac tccgttagcc cgtgccactc gtgctaccga
     2761 tgcccccccg gccaacgctt cccgaacaat ccggagcttc tcttccaggg atcgatactg
     2821 atgcttgcca ggtaccggtt gcgtgtaagt gtccatcatc acccccaaat ggacactacc
     2881 taagtgtcca cttcgcgagc atgactcaca cttgcaccgc tgtctacacg ggtcacaatc
     2941 ggacgcttac gtcgctgacc gggtgggccg gtgcgcagac caagcgtcac agcccggaga
     3001 gacgggtgta cgaaaggctg accacgagcg gacggcgcaa ccggtccggc gttgcgccag
     3061 gacacatccc cacaactgag gctaaccttt c
//
LOCUS       DASPUL010000242         7569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_450552, whole genome shotgun sequence.
ACCESSION   DASPUL010000242 DASPUL010000000
VERSION     DASPUL010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7569)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7569
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450552"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1843)
                     /locus_tag="VEJ00_06095"
     CDS             complement(272..1843)
                     /locus_tag="VEJ00_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXX00764.1"
                     /translation="MATDIQVAPRLISSLNPATGELLRELDCAGTAEVEAAVARARSA
                     QAAWAGVGLRRRIAILREFQHRLYARKSEIASAITREAGKPLVEALVTEVLVVLDAAR
                     FLIDNAFSLLRDEPVPHANLVTKLKSGWLVREPHGVIGIVSPWNYPFSIPATETLAAL
                     VAGNAVVLKPSELTPLVALELASLLYAAGVPEDVFQVIVGEGPAGAALLCSPIDKLVF
                     TGSVATGKRIAAAAAERLLPVVLELGGKDPMLVLDDADVDVASSAAVWGAFVNAGQAC
                     LSVERCYVHHRLYEPFAEACARKTKQLRVGNGMDSHTDVGPMIRERQLRIVEAHVEDA
                     RAHGARVLAGGRRLPELGQNFYAPTVLAGVTQDMRIMREETFGPVLPVMSCAGDEEAV
                     RLANDSEYGLAASVWTRDRARGERLARRIHAGTVMVNDVVSCFGISEAPHGGVKASGL
                     GRTHGRFGLDEMVRVKYLDMDRMPGMKKVWWHGYGESFRRQMEGFLDMQFARSMTARV
                     RGALRAAGILRRKQL"
     gene            complement(1997..2749)
                     /gene="truA"
                     /locus_tag="VEJ00_06100"
     CDS             complement(1997..2749)
                     /gene="truA"
                     /locus_tag="VEJ00_06100"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXX00765.1"
                     /translation="MRNLKLILSYDGAEFSGWQVQPDAATVQGTLASAIGRVTGEKVL
                     PQGSGRTDAGVHALAQVATFTTESSVPTENFVKALNDILPAAMRVLEVTEMPAEFHAR
                     HSARAKTYRYRLYRGAICSPFLARYVWHYPYPLDELAMARAAFLVVGEHDFTSFAAVD
                     PERGREDEPASNVRRISSSAWERADEELVYTVRGSGFLHHMVRNLVGTFILVGRGTKQ
                     VDDVTRILEARNRAAAGATAPASGLHLVNVEY"
     gene            3224..5287
                     /locus_tag="VEJ00_06105"
     CDS             3224..5287
                     /locus_tag="VEJ00_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXX00766.1"
                     /translation="MPFARRTLTVRYAAAVAGLFAALSLGCRGQESSSATAAQPSSQK
                     PLPVLDFSKPVSHFPNPIGPYTPRHLAPPNLANTARIDQLMKDGKLYLSLDDAIALAL
                     ENNLDIAIARYNLNIADTDVLRAKAGAPTLGVNTGIVQNTPGGGVGGVGGQVGSGTGG
                     TSLGAGGAGAGAGGLVGSTLGIGPQITSFDPVVTGTLQEDHFKQLATTIFQGVPPGTS
                     LVQNTGSVNFAYQQAFSWGTNLNVGFTNTRGTTNSFFQGLSPQLVSGFKATFTQHLLQ
                     GFGFAPNTRFIRIAKNNRELSDVAFRLQVITTVDQIENMYWDLVYAYENLRVKQESLT
                     FSEKTLSDTKKQVEIGSLAPIETVRAQSTVAADEQALTVAKTNLQLQQLLMKNALSRT
                     LHDPTLAGAEVIPMSTMEVPENEQIQPTEDLVNEALRHRAELVESRIQLNSQEISNKA
                     VRSALKPTVDLFAYYGGAGLGGTQNPANLCANANQSDCAINNPDLQLGQSPISPSTSY
                     GGTLNQLVNSTSPDKGLGLQLNIPLRNRAAEAVQIRSELEFRQAQMALQQTENRISIE
                     VRNAQFGVEQNRASVASAQAAVDFAKQSLDAEQKKYQFGTSTTTLVLQNRSALASAES
                     TLLSAMAAYEKSRLELDRATGQLLDHASISIDDAAKGQVTHLPNVPYVAPRKDLPSAA
                     TQPQQ"
     gene            5583..>7569
                     /locus_tag="VEJ00_06110"
     CDS             5583..>7569
                     /locus_tag="VEJ00_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXX00767.1"
                     /translation="MFRSSLKFLQKQLWCVTSALALGIIPGVVRAQQPLPAASAPTAP
                     SAPQPAEAQLVHLKDYSKPRATFPRLLQPYMPDQLAQPSLGNSPRIDSLMHDGKIYLS
                     IDDAVALALENNLDLEIARYNLDIAGADLLRAKSGASILGINTGVVQNTPGGGVGGLG
                     GTVGSGTGGTTVAAGGAGTGTNGLVSSTLGVGAPITSFDPQLTSTLQVDKNNTESVSV
                     FSPVPVVAQNTYTANFAYSQGFDWGTTLSAGFNNTHVTTNNPTSLLTPQLGSNFQFRL
                     TQNLLQGFGLLPNTRFIRIAKNNREISDVAFRLQVITTVDQIENMYWDLVFAFENVRV
                     QRESLTYAQKALDDAQRQAQVGTIPPIQVVSAQSTVATDQQNLIVAQNNLQLQQLLMK
                     NAVSRSIEDPTLAEADVIPTSTIQIPQEEPVIPIQDMISEALNHRAELVESRIDLNSR
                     DINNKAVRNSMLPTVDAFAYYGGSGVGGSVNPFVPVCSATSSKFCFDQATAPPPFQTT
                     TRVTYGGTLNQLVNSTAPDKGIGLTLNIPIRNRLAQSNQVRAELEYRQALVREHQLEN
                     QVRIEVRNAGFDVKQNRVAVQAAQSAVDLARQTYDADQQKLKVGLTTQTAILQDAATL
                     TTAESNLVSAKAAYEKSRIELDRATGLLLDHAGIDIAD"
BASE COUNT         1510 a         2387 c         2293 g         1379 t
ORIGIN      
        1 gggcagcgga attcggatct tgcccgggac gcggcatcag attcgcaatc cgtcgtcggg
       61 gccggcgcgg tttctggtca tttcgcaacc cccgagtcac ggggaccggg tgaacgaata
      121 gaaccgcagg ggctaaagcc cctgacattc tttccacgct ttacgcggcc ctaaaggggc
      181 gctcttccac ggttgccttg gcgtgcgact gggagaacgc tctggcccca ttccccatgg
      241 gcgcgtcgtg tagacggcgc tatggctcgc gtcacaactg cttgcgccgg agtatccccg
      301 ctgcccgcaa ggcgccgcgc acccgcgcgg tcatgctgcg ggcgaattgc atatcgagga
      361 aaccttccat ctgccggcgg aagctctcgc cgtagccgtg ccaccagact tttttcatgc
      421 cgggcatgcg atccatgtcg agatatttca cacgcaccat ctcgtcgaga ccgaagcgtc
      481 cgtgggtgcg gccgaggccg ctggctttga cacctccgtg cggagcctcg ctgatgccga
      541 agcaggagac cacatcgttg accatgactg tgccggcgtg gatgcggcgg gcgaggcgct
      601 cgcctcgagc acggtcgcgg gtccacacgc tggcggccag gccgtattcg gagtcgttgg
      661 ccagacgcac agcttcttcg tcgccggcgc aggacatcac tggcagcacc gggccgaagg
      721 tttcttcgcg catgatgcgc atgtcttgtg taacgccggc gagcacggtg ggcgcgtaga
      781 agttttgccc cagctcgggc agccgcctgc ctcccgccag aacccgcgcg ccatgcgccc
      841 gggcatcctc gacgtgtgct tcgacaattc gcagctgccg ctcgcggatc atcggaccga
      901 cgtcggtgtg ggaatccatg ccgttcccca cgcgcaactg cttcgtcttc cgggcgcatg
      961 cttcagcgaa cggctcgtac aggcggtgat gcacgtaaca gcgctcaacc gagaggcacg
     1021 cctgcccggc gttgacgaag gcgccccaga cagcggcgct ggaagcgacg tcgacgtcgg
     1081 cgtcgtcgag caccagcatg ggatctttgc ccccaagctc gagtacgacc ggaagcaacc
     1141 gctcagcggc ggcggcagcg atgcgcttgc cggtggcaac actgccggtg aagaccagct
     1201 tgtcgatggg agagcagagc agtgccgcac cggccgggcc ttcgccgacg atgacctgaa
     1261 acacatcttc gggaactcct gccgcgtaga gcaacgacgc cagttccagt gcgaccagcg
     1321 gcgtcagctc ggaaggcttc agcacgacgg cgtttccggc tacgagagcc gcgagtgtct
     1381 cggtcgcggg aatcgagaat ggatagttcc agggcgagac gataccgatc acgccatgcg
     1441 gctcgcgcac cagccatccg cttttcagct tggtgacgag attggcgtgt gggacaggct
     1501 cgtcgcgcag caggctaaaa gcgttgtcga tgaggaagcg cgcagcgtct agcacaacga
     1561 gcacttcggt gaccaacgcc tcgaccagcg gtttgccggc ttcgcgggtg atggcggagg
     1621 cgatctccga ttttcgtgcg tacagccggt gctgaaattc ccgcaggata gcgatgcgcc
     1681 gacgcagtcc gaccccggcc caggcggctt gtgccgagcg cgctcgcgcc acggcggcct
     1741 cgacctcggc cgtgccggca caatcgagct cgcgcagaag ttcgcccgtc gcgggattga
     1801 gcgatgagat caggcgcggt gcgacttgta tgtcggttgc catacgcaaa ttctagtcct
     1861 cggcagttgc tctgctgtgt ctctggtcac cagacagtta tgggccgggt cagacagcag
     1921 gttcctcgtc actccgctcc tcgggttgac aacggggtag ggtcgcgttc tctcctaatc
     1981 aattcgtccc tgcccatcaa tactcgacgt ttaccaggtg gaggccgctg gccggggccg
     2041 tcgcgccggc ggccgcgcgg ttgcgcgctt ccagaatgcg cgtgacatcg tccacttgct
     2101 ttgtgccgcg gccgacgagg atgaatgtgc ctaccagatt ccgcaccatg tggtgcagga
     2161 atccggagcc acggacggtg taaaccaact cctcgtctgc tcgctcccag gcagaggaag
     2221 aaatccttcg cacgttcgac gcgggctcat cctctcgtcc acgttccgga tcgactgcgg
     2281 caaaggaggt gaaatcatgc tcgcccacaa ccagaaacgc ggcccgcgcc atcgccaact
     2341 catcaagcgg gtacggatag tgccacacgt agcgtgccag aaacggagag cagatcgcac
     2401 cacgataaag tcgatagcga taggtcttgg cacgcgccga atgccgggcg tgaaactcgg
     2461 caggcatctc cgtgacttcg agcacccgca tcgccgccgg caaaatatcg ttgagagcct
     2521 taacgaagtt ttccgtggga accgaagact cggtggtgaa cgtggcgacc tgcgccagcg
     2581 cgtgcactcc ggcgtcggtg cggcctgagc cctgcggcaa gactttttct ccggtgacgc
     2641 gtccaatggc cgaggccaga gtgccctgca cggtggctgc atccggctgc acctgccagc
     2701 cggagaactc agcgccgtca taggagagaa tcaatttgag attgcgcatg gcgcggatgt
     2761 tgtctgatgg tctctgtttt tgagcgtcgc tcttgagcgc cagtcgtatc tttcgagtcc
     2821 tcaattacgt ttgcatcatc caacaaattt tgggcttgcg cgcatccatt cgcaggactc
     2881 gggattctcc gagcactgta ccagttccgc gaaccggggc gcagccggag ctgcacatcc
     2941 cggtcggata tactggcccg gttcccaggg atcgagccca tgggcagaat tcaccaggcg
     3001 cgttgcgcgt aagatcgcgg cgcgcggaaa gatggcagcg aaacgaatgc tgcggcgggg
     3061 gaatgccggg atcaccttcc gggaaccttc cgccccgctt ttgcgtaccg attcctgagc
     3121 gacgtgtcga attctagccg gttcgttcga ctcaggttca aacccggttt tcttaagctg
     3181 caggttttca tcaatctggt ctaaatcaac gggagatgag tagatgccat tcgcacgcag
     3241 aaccctgact gtccggtacg ccgcagcggt tgccggtttg tttgccgcgc tgagcctggg
     3301 gtgccggggg caagagtctt cctcggccac ggcggcgcaa ccgtcgtcgc agaagccgtt
     3361 gcccgttctc gatttcagca agcccgtgtc gcactttccc aatccgatcg ggccgtacac
     3421 gccgcgccac cttgcgccgc cgaatctggc caacacggcg cgcattgacc agctcatgaa
     3481 ggacgggaag ctgtatttgt cactcgatga tgcaatcgcg ctggcgctcg agaacaatct
     3541 cgatatcgct atcgcgcgct acaacctgaa cattgccgac accgacgtgc tgcgcgccaa
     3601 ggcgggcgcc ccgacgcttg gcgtcaatac cggtatcgtg caaaacacgc cgggcggcgg
     3661 cgtcggcggg gtgggcggac aggtcggctc gggcacgggc ggcaccagtc tcggtgcggg
     3721 cggggcgggc gccggtgccg gtggcctggt gggatcgacg cttgggatcg ggccgcagat
     3781 tacgagcttt gacccggtgg ttacaggcac actgcaggaa gaccatttca agcagctggc
     3841 caccacgatc ttccagggtg tgcctcctgg taccagtctg gttcagaaca ccggcagtgt
     3901 gaattttgcc taccagcagg cctttagctg gggcacgaac ctgaatgtgg ggttcactaa
     3961 tacgcgggga acgactaata gcttcttcca aggtctgagc ccgcaactag tttcaggctt
     4021 caaggcaaca ttcacgcagc atcttctgca aggatttggg tttgcgccga acactcgctt
     4081 catccgcatc gccaagaaca atcgcgaact ttccgacgtt gccttccgac tgcaagtcat
     4141 cactaccgta gatcagatcg aaaatatgta ttgggacctc gtctatgcat acgagaatct
     4201 gcgggtgaag caggaatcgc tgactttctc cgagaagacc ctttctgaca cgaaaaaaca
     4261 ggtggagatc ggaagcctcg cgccgattga aaccgtgcgc gcacagagca cagtcgctgc
     4321 cgatgagcag gcgctaacgg tcgccaagac gaatctgcag ttgcagcagc tgctcatgaa
     4381 gaatgcgctc agccgcaccc tgcatgatcc cacgctggcg ggagcggaag tgattccgat
     4441 gtcgaccatg gaggttccgg aaaacgagca gatccagccc accgaggacc tggtcaacga
     4501 agcgcttcgc catcgcgccg aactggtgga gtcgcgcatt cagttgaata gccaggaaat
     4561 cagcaacaag gcagtgcgga gcgcgctcaa gcctacggtc gatctgtttg cttattacgg
     4621 cggcgccggc ctgggcggta cgcagaaccc ggccaacctc tgtgcaaatg ccaatcaatc
     4681 ggattgcgcc atcaacaatc ctgacctcca actcggccaa agcccgatct cgccgtccac
     4741 gagttacgga ggtacgctca accagctcgt gaattccact tcgcctgaca aaggcctggg
     4801 cctgcagctc aacattccgt tgcgcaatcg tgcggccgag gcggtacaga tccgctccga
     4861 gctggagttc cggcaggcgc agatggcgct gcagcagacc gaaaaccgca tcagcattga
     4921 agtgcgcaac gcgcagttcg gcgtggagca gaaccgcgcc agcgtggcat cggcgcaggc
     4981 ggcggtggat ttcgccaagc agtcgctcga tgctgagcag aagaaatatc agttcggcac
     5041 ttcgaccacg acgctggtcc tgcagaaccg cagcgcgctg gcatcggccg aatcgacact
     5101 gctgtcggcg atggcggcct acgagaaatc gcgactcgag ctggaccgcg cgaccggcca
     5161 gctgctcgat cacgccagca tcagcatcga cgacgccgct aaaggacagg tcacgcacct
     5221 gcctaacgtg ccctacgttg cgccccgcaa ggacctgcca tcggccgcga cgcagccgca
     5281 acaataagtc gtgtggcgcg ggcactcctg cccgcgcccg cctcgcatgg gcaccagatg
     5341 tacgcgggca agaatgcccg cgccacactc tgctcacaat tctttacaat cccgtccggt
     5401 gtgttgatct cgatcccgct aagatgaagg gaacgcttcc tcgggcaacg aggagttgaa
     5461 caagcctcag gggctaaagc gcgcaacttt tcggcggctt tggcggcacc gcgagagcca
     5521 tgctcttccc aaatcacata cgagttggga tgcagacccg ttctcatgca aggagaagac
     5581 ccatgttccg atcgtctctg aaattcctcc agaagcagtt gtggtgtgtg acttctgccc
     5641 tcgcgttggg gattattccc ggcgtggtac gcgcgcagca acctctcccg gccgcatcag
     5701 cgccaacggc gccctctgct ccgcagccgg cggaagcgca actggtccat ctgaaggact
     5761 attccaaacc gcgcgcgacg tttccccgcc ttttacagcc ctacatgccc gaccagctgg
     5821 cgcagccgag cctgggcaac tcgccgcgca tcgactcgct catgcatgat ggcaagattt
     5881 acctctccat cgacgacgcg gtcgcgctcg ccctcgagaa caatctcgat ctcgaaatcg
     5941 cccgctacaa cctcgacatc gccggagctg atctgctgcg cgccaagtcg ggagcgagca
     6001 ttctgggaat taatacaggc gtggtgcaaa acacacctgg tggcggcgtc ggcggactgg
     6061 gcggcaccgt cggctcgggt acaggcggca ctaccgtcgc cgccggcggc gcaggtaccg
     6121 gcaccaacgg cttggtcagc tcgacgctcg gcgtgggcgc gccgatcacc agttttgatc
     6181 cgcagctgac cagcacgctg caggtggata agaacaacac ggaatcggta agcgtcttca
     6241 gcccggtccc ggtcgtcgcg cagaacacct acacggccaa cttcgcctac agccagggat
     6301 tcgactgggg cacaacgctg agcgcgggat tcaacaacac gcacgtcacc accaacaacc
     6361 ccaccagctt gctgacaccg cagctcggct cgaatttcca gttccggctt acccagaatc
     6421 tgttgcaggg tttcggcttg ctcccgaaca cgcgttttat ccggatcgcg aagaacaatc
     6481 gcgagatctc cgacgtcgcc tttcgcctgc aagtcatcac caccgtggat cagatcgaaa
     6541 acatgtactg ggacctggtc ttcgctttcg agaatgtgcg cgtgcaacgg gaatcgctta
     6601 cctacgccca gaaggcgctg gacgacgcgc aacggcaggc gcaggtgggc accattccgc
     6661 ccattcaggt cgtgagcgcg caaagtacgg tcgccaccga tcagcagaac ctgatcgtgg
     6721 cgcagaacaa cctgcaacta cagcagctgt tgatgaagaa cgccgtcagc cgcagcattg
     6781 aggatccgac gctcgctgag gctgacgtta ttcctacctc gaccattcag attccgcagg
     6841 aagagccggt cattcctatc caggacatga tcagcgaagc gctaaatcat cgcgcagagc
     6901 tggtggaatc gcgcatcgac ctcaattcgc gcgacatcaa caacaaggcg gtacgcaact
     6961 ccatgctgcc gacggtggac gcgtttgcct actatggcgg gtcgggcgtg ggaggctcgg
     7021 tcaacccgtt tgttccggtc tgctcagcga cctcgagcaa gttctgcttt gaccaggcca
     7081 cagctccgcc gccttttcag accacgacga gggtcaccta cggtggaacg ctcaaccagc
     7141 tggtcaattc gaccgctccg gacaaaggca ttggcttgac gctgaatatc ccgatccgca
     7201 accggctggc gcagtcaaac caggtgcgcg ccgaactgga ataccgccag gcgctggtgc
     7261 gcgagcatca gctggagaac caggtgcgca tcgaggtgcg caacgccggg tttgacgtga
     7321 agcagaaccg ggttgcggtg caggccgcgc aatcggcggt cgacctggcg cgccagacct
     7381 atgatgcgga tcagcagaaa ctgaaggttg gcctgaccac ccagaccgca atcctgcagg
     7441 acgccgccac gctgacaacg gcggaatcga atctggtttc ggccaaggcg gcatatgaga
     7501 agtcgcgcat cgagctcgac cgagcaaccg gcttgctgct ggatcacgcc ggcatcgaca
     7561 ttgccgacg
//
LOCUS       DASPUL010000243         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1271500, whole genome shotgun sequence.
ACCESSION   DASPUL010000243 DASPUL010000000
VERSION     DASPUL010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271500"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VEJ00_06115"
     CDS             <1..348
                     /locus_tag="VEJ00_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00768.1"
                     /translation="VRRLETPFGQFTLLEIKIDTGRTHQIRVHMASLGHPIVGDKLYG
                     APRELRGLPRSGDANGAASLSRNFLHAAKLELAHPRTGQKIALETALPEELEGFLNML
                     EASRGQARSGRRH"
     gene            432..1496
                     /locus_tag="VEJ00_06120"
     CDS             432..1496
                     /locus_tag="VEJ00_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXX00769.1"
                     /translation="MHRAAGLLPLMLLTLGAVAQSSGQSAPQATTLSASPQQTQPGNQ
                     AAGATAQPIPQQPAQGSDSADEPVTKITINPTEVNVVFTVTDKHGKRVTDLKQADFRI
                     VDDNKAPAEIHSFRAETNLPLQVGLLIDASNSVRDRFKFEQESAIEFLNQTVRPHYDL
                     AFVVGFDVTPEVTQDFTDNTEALAHGVHELRPGGGTALYDALYFACRDKLLKAPKITA
                     VRRAIILLSDGEDNQSHVTREEAIEMAQRAEAIVYTISTNVSGTKGSGDKVLERIADA
                     TGGRAFFPFQIRDVANAFAEIQDELRSQYSVAYKPADLKYDGHYRTIEIMANDHKNLR
                     VRSRRGWYSPMAEPSTGVNR"
     gene            1511..1915
                     /locus_tag="VEJ00_06125"
     CDS             1511..1915
                     /locus_tag="VEJ00_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00770.1"
                     /translation="MPGYSGTPLPQKLGIKGGFEVRLINAPADVRAELKAALTRCEVR
                     SDGRAPLDFAMLFTTSKAELAREFGKIGKVLAPAGMLWVSWPKKSSGVATDLDENVVR
                     KIGLAAGLVDVKVCAVTEIWSGLKFVRRLKDR"
     gene            1949..>2994
                     /locus_tag="VEJ00_06130"
     CDS             1949..>2994
                     /locus_tag="VEJ00_06130"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXX00771.1"
                     /translation="MVRVGLIGFGLAGQAFHAPVIRGVSGMELACILERHTDRARQKY
                     PDVRIARTLDELFSDTTIGLCVVATPNDSHFSYAKACLDAGRHVVVDKPFTPTMAEAE
                     RLVHLAAERGRLITVYQDRRWDGAFLTLKKLVASGSLGKIAEYEARFDRFRLDPKPNA
                     WREKANFPAAGVLWDLGPHLIDGALVLFGEPESIWASAFCQRPTSAIDDAFDVCMGYP
                     GLRATLRARIIAFAPSHHLLLHGTAGSFVKCGMDPQEEILRSSNCPDGPAWGDDWGKE
                     PPERWGTLSRVGGEIRKVETERGDYRGFYANVRDAIEKNAKLDVTPEQALRTMRALLL
                     AHKSSRESRVVRWT"
BASE COUNT          632 a          892 c          918 g          552 t
ORIGIN      
        1 gtacgccggc tggagacgcc cttcgggcaa ttcacgctgc tcgaaatcaa aatcgacacc
       61 ggccgcaccc accagatacg cgtccatatg gcctcgctcg gacacccgat cgtgggagac
      121 aagctctacg gcgctccgcg agaactcaga ggtctaccgc gatctggaga cgcgaacggc
      181 gcagcgtcgt tatcccggaa ttttctacat gccgccaaat tggaactggc ccacccgcgg
      241 actggtcaga aaatcgctct tgagactgcg ctgccggagg agttggaggg gtttctgaac
      301 atgctggaag cgtctcgcgg acaggctcgg tcgggccgga gacattgacg ctcacggtgc
      361 tgcaagtgaa cgtttcggga ccgcggccgg atataatgta accagagatg cacgtcttgg
      421 ggacaagaat catgcacagg gctgccgggt tgctcccgct gatgctgctt accctgggag
      481 cggttgcgca gtcgtctggg cagagtgcgc cgcaggcaac cacgctgtct gcttctcccc
      541 aacagacgca gccaggcaac caggcggccg gtgcaacggc gcagccgatt ccgcagcagc
      601 cggctcaggg aagtgattcc gctgacgaac cggtcaccaa gatcacgatc aatccgaccg
      661 aagtcaacgt cgtgttcacc gttaccgata agcacggcaa acgtgtgacc gacctgaagc
      721 aggccgattt ccgcatcgtc gacgataaca aagctccggc tgagatccat agctttcgcg
      781 ctgagaccaa tctccccttg caggtgggtt tgctcatcga cgccagcaac tcggtgcgcg
      841 accggttcaa gttcgagcag gaatcggcaa tcgagttcct gaaccagacc gttcgaccgc
      901 attacgacct ggctttcgtc gtgggtttcg acgtgactcc cgaggtcacc caggatttca
      961 ccgacaatac ggaagccttg gcccacggcg ttcacgaact gcgccctggg ggcggaaccg
     1021 cgctctacga tgcactttac tttgcctgcc gcgacaagct gctcaaggcg ccgaaaatca
     1081 cggcggttcg ccgcgccatt attttgctga gtgacggcga ggacaaccag agccatgtca
     1141 cccgcgagga agccatcgag atggcgcagc gcgccgaagc catcgtctac accatcagca
     1201 caaatgtgag cggcaccaag gggtcaggag acaaagtcct ggagcgcatc gccgacgcga
     1261 ccggcgggcg cgccttcttc cctttccaga ttcgcgacgt cgccaacgct ttcgccgaga
     1321 ttcaggacga gttgcgcagc cagtactcgg tggcgtacaa gccggccgac ctcaagtacg
     1381 acgggcacta tcgcaccatc gagatcatgg ccaacgatca caaaaatctg cgggtgcgct
     1441 cgcggcgcgg ctggtactcg ccaatggccg agccttccac cggcgtcaac cgctaggatc
     1501 ggccagcgtc atgccgggct actcgggaac accactaccg caaaaactgg ggatcaaggg
     1561 cgggtttgaa gtgcgcctca tcaacgcgcc tgcagatgtg agggcggaat tgaaggccgc
     1621 actcacacgt tgcgaggtca gaagtgacgg aagagccccg ctcgactttg ccatgctgtt
     1681 cacaacgtcg aaggcagaac tggccaggga attcggaaaa atcggaaagg tcctcgctcc
     1741 tgcaggaatg ctgtgggtca gctggccgaa gaaaagctcc ggcgtggcaa cggatctcga
     1801 cgaaaatgtc gtccgcaaaa tcggtctggc agcgggtttg gttgatgtga aggtttgtgc
     1861 tgtcactgaa atctggtcgg gattgaaatt cgtgcggcgc ctgaaagatc gctgagatag
     1921 aagtcaccgg gattgaggca agatacgcat ggtgcgagtg ggcctgatcg gattcggcct
     1981 ggcggggcag gcgtttcacg cgcctgtcat tcgcggcgtc tccggcatgg agttggcctg
     2041 catcctcgag cgccacacgg atcgcgcgag acagaagtat cccgatgtcc gcatcgcacg
     2101 tactctcgat gaacttttct ctgatacgac gatcggattg tgcgttgtgg ccacgcccaa
     2161 cgactctcat ttctcgtatg ccaaagcctg tctcgacgct ggccgccacg tggtcgtcga
     2221 caagccattc acgccaacga tggccgaggc cgagcgtctc gtacacttgg ccgccgagcg
     2281 cgggcggcta atcaccgtct accaggatcg ccgctgggac ggtgcctttc tcacgttgaa
     2341 gaaactggtg gcttcaggct ccttgggcaa gattgccgag tatgaggcgc gcttcgatcg
     2401 cttccgtctg gacccaaagc ccaatgcctg gcgggaaaaa gcgaactttc ctgcagcggg
     2461 agttctctgg gatctcggcc cgcatctgat cgatggcgct ctggtattgt tcggtgaacc
     2521 agagtcgatt tgggcgtcgg cattttgcca gcgaccgaca tcggcgatcg acgatgcatt
     2581 tgacgtttgc atggggtatc cgggtctgcg ggcgacgttg cgagcgcgca tcatcgcgtt
     2641 cgctccgagt catcacctgc ttctacacgg cacagcggga tcgttcgtga aatgcggcat
     2701 ggatccgcaa gaggaaatcc ttcgcagctc aaactgcccc gacggtcctg catggggcga
     2761 cgattggggc aaagagccgc cggagcgctg gggaactctg agtcgcgtcg gcggagagat
     2821 ccgaaaagtc gagaccgagc gcggagacta ccgcggcttc tacgccaacg tccgcgatgc
     2881 gatcgagaaa aatgcaaaac tggatgtgac cccggagcag gccctgcgca ccatgcgcgc
     2941 actgctgctg gcccacaaga gcagccgcga aagccgagtc gtccgatgga cgga
//
LOCUS       DASPUL010000244         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1001213, whole genome shotgun sequence.
ACCESSION   DASPUL010000244 DASPUL010000000
VERSION     DASPUL010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001213"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VEJ00_06135"
     CDS             complement(<1..1394)
                     /locus_tag="VEJ00_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXX00772.1"
                     /translation="MHAINLEAHPTAPAGVDPLERISPDLPALANRNWAEIFDFISDL
                     IVVHDQENRVLRVNRSLAELIGVAPAELSGVQMQALLSFPGDAGSGPCPICRLALAGA
                     EEFAHPSLERTYLVSTSCLRAPDRGWQTLHVLKDITDRRAAERRYRELFDNIQEGVFF
                     STPEGRFVEVNDALVRMLGYHSREELLQTDIPHQLYPSPAHRQRFLETIEANGAVHNF
                     EQVLRRKDGHLVYALENAFAVRDAQGKVVQYRGVVLDITELKVSQARLQRERDFTTKI
                     LNGTQSLILVTDRSGLITYANRRWFAAGYSQERLLNLPLLELVAPAAQEAMARALEST
                     LAGQNVDNLELAMVRGDDSHGRFCLNLSPMRDEQGNVVSIIVVMTDITDAEVLKANLM
                     HAEKMASVGQLVSGVAHEVNNPLTAILGFADLLLQRDLDAGARKELHVIVHEAQRTKQ
                     IVQNLLSFARQTAP"
     gene            complement(1589..>2414)
                     /locus_tag="VEJ00_06140"
     CDS             complement(1589..>2414)
                     /locus_tag="VEJ00_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00773.1"
                     /translation="AVGTGPNYIVYEPILNRAYVTSPPDGSISIIENATAAPPAVPHV
                     TKVPLAPAPCNGTTPISVTALADGTRAYVADQAGNSVCILNTTSNTFTKRICLVQEPA
                     VSTDPCVAGAAPAFIASDSNSFRVYTANQFQTAAFAISSISRQNITNPVTGVVTGLVT
                     VTTTSSVPFQAGQPVTISGVQFDTSFNGVFGITSITAPNRFTYVQTGLLNSASAAPGA
                     SATAAVLPYVSLIQTANDSLVPNDNEATDNASLNLITPPLTIDAGGTPTFIVMTPF"
BASE COUNT          437 a          690 c          838 g          449 t
ORIGIN      
        1 tgcggcgcgg tctggcgggc gaagctcagc aggttctgca cgatctgctt ggtgcgctgc
       61 gcctcgtgca cgatgacgtg cagctccttg cgggcgcctg catcgaggtc gcgctgcaat
      121 agcaggtcgg cgaagccgag aatagccgtc agcgggttgt taacctcgtg ggccacgccg
      181 gaaaccagtt gtccgactga ggccatcttc tcggcgtgca tcaggttggc cttcagcacc
      241 tcggcgtcgg tgatgtcggt catgaccacg ataatgctga cgacgttgcc ttgctcgtcg
      301 cgcatgggac tgagattgag gcaaaagcgg ccatggctgt catcgccgcg caccatcgcc
      361 agctcaaggt tgtcgacgtt ctggccggcg agggttgatt ccagagcgcg ggccatcgct
      421 tcctgcgcgg ccggagccac cagctccagc agcggcaggt tcagcagacg ttcctggctg
      481 tagcctgcgg caaaccagcg gcggttggcg taggtgatca gcccgctgcg gtcggtcacc
      541 aggatcaggc tctgggtgcc gttcaggatc ttggtggtga aatcccgctc ccgctgcaac
      601 cgcgcctgcg agaccttcag ttcagtgatg tcgagcacca cgccgcggta ctgcaccacc
      661 ttgccctggg cgtcgcggac ggcgaatgcg ttctccagcg cgtacaccag gtggccgtcc
      721 ttgcgccgca gcacctgctc gaagttgtgc accgcgccgt tcgcttcaat ggtctccagg
      781 aacctctggc ggtgggccgg cgacgggtag agctggtggg ggatgtctgt ctgcagcaat
      841 tcttcccggc tgtggtagcc gagcatgcgc accagcgcgt cgttgacttc gacgaaccgg
      901 ccctcgggcg tggaaaaaaa gacgccctcc tggatattgt cgaacagttc gcggtagcgc
      961 cgctctgccg cgcgccggtc agtgatgtct ttgagcacgt ggagggtctg ccagccgcgg
     1021 tcgggggcgc gcaggcacga ggtggaaacc agataagtgc gctccagcga cgggtgcgcg
     1081 aattcctcgg cgcctgccag ggccaggcgg cagatagggc aggggccgct gccggcgtcg
     1141 ccggggaacg agagcaaggc ctgcatctgc accccgctca gctccgccgg ggccacgccg
     1201 atgagttctg ccagcgaacg gttcacgcgc agcacccggt tctcctgatc gtgcaccacg
     1261 atgaggtcgg agatgaaatc gaagatctct gcccagttcc ggttcgcaag cgcgggaaga
     1321 tcaggagaga tccgctccag agggtcgacg ccggccggag cagtggggtg ggcttccaag
     1381 ttgatcgcgt gcatgggccc tcgggcaggc gccaatatct gcccgggata acgcggcggt
     1441 ggaagcgatt ccaaagccga agctaatcgc gaatctcaat ttgagtcaag ggaatagaac
     1501 gacatgtgcc caggtcacaa agaggtaggg aggaacggaa tgattagtct caatttgaaa
     1561 tacggccaca gggcagcccg cccggctgtc agaacggcgt catgacgatg aatgtaggcg
     1621 tgccgccggc atctatggtc agcggcggtg ttatcagatt cagactagcg ttgtcagtcg
     1681 cctcgttgtc gttcggcacc aggctgtcgt tggcggtttg gatcaaggag acgtaaggca
     1741 acacagcagc cgtagcactc gcacccgggg cggcggaggc ggaattgagt aagccagttt
     1801 gaacgtacgt gaaccggtta ggggcggtaa tggaggtgat cccgaatacg ccgttgaagc
     1861 tggtgtcgaa ctgcacgccc gatatggtca ccggctgccc ggcttggaac ggaacgctgc
     1921 tagtggtggt caccgtgacc agccccgtca ccaccccagt caccgggtta gtaatgttct
     1981 ggcgggaaat cgacgagatg gcaaatgctg ccgtctggaa ttgatttgcc gtgtacacgc
     2041 ggaaggaatt ggaatcggag gcgatgaagg ccggtgcggc gccggccacg cagggatcgg
     2101 tcgacacggc cggttcctgt accaggcaga tgcgcttggt gaaggtgttg gacgtcgtgt
     2161 tgaggatgca gacgctgttg cccgcctgat ccgccacgta ggcgcgcgtg ccgtcggcca
     2221 gagccgtgac cgagatgggg gtcgtcccgt tgcagggcgc tggcgccagg ggaactttcg
     2281 tcacgtgggg tacggctgga ggagcagcgg tggcgttctc gatgatggat atgctgccgt
     2341 ccggcgggct ggtgacataa gcgcggttca ggattggctc gtacacgatg tagttgggcc
     2401 cagtgccaac ggcc
//
LOCUS       DASPUL010000245        14068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1957506, whole genome shotgun sequence.
ACCESSION   DASPUL010000245 DASPUL010000000
VERSION     DASPUL010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 14068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14068)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14068
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1957506"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VEJ00_06145"
     CDS             <1..444
                     /locus_tag="VEJ00_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00774.1"
                     /translation="HIPVWVAIAEIAATAFVVLSIVAIVVYAPFWILGGLSKKRRRPA
                     ERSMRVWPLLAVLSLIVFVTIFIVSGDDLIERMGNLTIWSAGVLLSSLAFAVTTIVSA
                     IAVYRAPAETVRKTVRRFSAVVTVALVIATLYLAYWGVIGLRTWA"
     gene            complement(450..3965)
                     /locus_tag="VEJ00_06150"
     CDS             complement(450..3965)
                     /locus_tag="VEJ00_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HXX00775.1"
                     /translation="MIWNRLKYRARRAFGLSKAGRNLTVFPDDIFIVSYPRSGNTWTR
                     FLIGNLLRPDDPVTFANVESRIPEIYFFTDRQLRRLARPRVLKSHEYFDPRYGRTIYL
                     VRDPRDVAVSLYKYSLKRRNIPDGYPIEEFIPRFIAGEFLVDWGNWQDHVRSWRAARK
                     EQSGFLLLKYEDLLADPQKELVRITSALKIDSSPARIARAVELSSAANMRNQEKKYSA
                     EWELTKDTRQDVPFVGEARAGGWQGKLPEASVLAIEHAWGATMEELGYPLSSNSPRAV
                     SASDASPSGTTPSLSDAERQTIVVDWNQTAVDYPRDIPLHRLVEEQVERTPDAVAVVS
                     GSQQLAYRDLNQRTNQLAHRLRKLGVGPDVLVAVCAERSLEMVIALLATLKAGGAYVP
                     LDPEYPADRLRTMLQDSKPPVVLTQAHLLARVPLSEAEVICLDRDWPSLTQENADNPS
                     PELAGKNLAYCIYTSGSTGKPKGVLNIHEAIVNRLLWMRDAYQVTKADRILQKTPYSF
                     DVSVWEFFLPLITGATLVMARPGGHKDPAYLVKLIQEQQITTLHFVPSMLALFLEADD
                     LDRCTSVRQVFASGEALSLELQSRFFERFHGKLHNLYGPTEAAVDVTCWECNPGYARP
                     VVPIGKPVANTQAYILNEQLQPVPVGVAGELHIGGIQLARGYLNRPDLTTEKFIPDPF
                     SQNANARLYKTGDLARFLPDGNIEFLGRLDDQVKLRGFRIELGEVEAVLSECAGVQLA
                     AVTVREDIPGDKRLVAYLMPVPGKDIPLDAVRVHLKEKLPEFMLPSRFVVLTEMPMTT
                     SGKVDRRALPRPKDERGEAREIVDARNGLESTILPIFRKVLRTQTLGVKDDFFELGGH
                     SLQAARLLSEIKRNTGHEIPLSAMLHAATVESLASLIASGGAASPDSVVTCIQRGENG
                     RAPLFAVVVPGVETIGYAALARHLGPRQPLYTLQARHPAPNGQPLTRSELEALAQDYI
                     SAMRSVQAGGPYHLIAMCDDVLVCEEMILQLERSGQQVGFFAILDTWPLQNTMVPWKW
                     KIDYYATRLRHAGKLPARDWLRSGREVLDRRRQKESSPRQSSRPTWGQIYWPGREFKP
                     HKFRAPVVLFKRPRQPFFYINDRAMGWSQRCLSKVELHEMELDHENLLREPHIKTIGG
                     ILAARLQIAEYPAQPPETLPSGLNR"
     gene            complement(3962..5788)
                     /locus_tag="VEJ00_06155"
     CDS             complement(3962..5788)
                     /locus_tag="VEJ00_06155"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXX00776.1"
                     /translation="MTSISKHRPVLVLGAAPRISLSIARSLNRGGIPVDIASLQPEDP
                     AIRSRAVRQFHRLPPPQDDPAAFSAALLGLVREQPFDLILPASDPALAALAGLYDVLS
                     PLLHVGCPPPSTVERVLNKSLTLQIAQRCGLRVPFTRSISSAAELDSISPQLRFPVVV
                     KPERKGSASAGRVFYFSSLADLSLALRSRGWGNVLLQEYCPGAGVGVEILIHNGECVA
                     SFQHRRLKEAPASGGVAVVAVSESVSPQLLESSMALLRALEWEGVAMVEFRVDSATGL
                     SVLMEVNGRFWGSVSLPILAGIDFPLYYWQLLHGEKPCVPPRYAVGLRWRWSPGCVER
                     IQSLLSRGGREVTPRRSKLMELLSALADFSPVTKEALWSWRDPAPFFAELGGAIRVFL
                     TSALKSASRRLLPRRRNSPADPLSRLTPEARSAYRSLRTHDFHLDSGNAHVVAGNAAS
                     FLFVCYGNLMRSPMAEAMFRHVLRERGINGIAVHSAGVHAVPGREAHPWALAVSRELS
                     LPLDQHRAQPVSKELVATSDAIFAMDFENLAELQTLYPAARDKIFLLSAFAEGEQRDR
                     EIHDPYFESLDGTRRCYSVLRTCIDNLAREIAASRPEQTRRP"
     gene            complement(5843..7399)
                     /locus_tag="VEJ00_06160"
     CDS             complement(5843..7399)
                     /locus_tag="VEJ00_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HXX00777.1"
                     /translation="MKFDKGQILRNVGSSWFALGVNVLVGIFLSPYILHRLGDTAFGL
                     WILIFSVTGYYGLFDLGIRSSIVRYVAKFAATGERDELDRLVSTALASYSCIGAVTML
                     ITLVAAGYVNAIFKIPPEFVNTARWLLLIVGAAVSLGFPFAVFSGILEGLQRFYLLNF
                     TTITATLLRALLIVIALRNGRGLLTVALITVSMPLMGGLINGAAVFRHLPLRLGIGYV
                     KRSTLSQIASYSGTTFVIIVAGRLRFKTDALVIGTFLSAAAVTYFTIGARLVDYATEV
                     VQSMAQIFVPMSSQSDALGDLQRLRQIFVAGNRMCALVIFPITAILVLLGKSVIEAWM
                     GARYVAASYPILLILLIPSTLMIMQAASSRVLFGMGKHQMLAKVTLAEGIANLVLSII
                     LVRKYGIVGDALGTAIPLSCTVLFFLPHHLCRLLKLRLLSYLRHSFLLPLGLTVPLVG
                     ALLLLRRWFVPHHLVPLAEQTVIALGVYGIGLAWAIWTRKAWQVRGLSYDERDAEIGI
                     ELAETSRQEA"
     gene            7687..8130
                     /locus_tag="VEJ00_06165"
     CDS             7687..8130
                     /locus_tag="VEJ00_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00778.1"
                     /translation="MNKTLRQRAVLDTLRHGHISSQEELQRVLRRRGFRVGQATLSRD
                     IRDLNLSKTAHGYALPQGEGSAGVALPPVSRLVREFVLDVRSAQNQLVIKTIVGSAQP
                     VAAALDEADWPEVVGTIAGDDTILIVCPDKEEAGKLAGRIEEMLS"
     gene            8132..9187
                     /gene="argC"
                     /locus_tag="VEJ00_06170"
     CDS             8132..9187
                     /gene="argC"
                     /locus_tag="VEJ00_06170"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HXX00779.1"
                     /translation="MTRKMTAKLKTAVLGATGYSGLELTRILLRHPQVEKPVLLRRSS
                     DNGTRDLAEVFPALSGNGGYPLQPLSWPTLKQQGVELLFLATPHEASRALVPEAIAHG
                     LRVVDLSGAWRLKQEQHRAIYSFQDANPVTAAELTAKAIYGLPELNADRLANATLVAN
                     PGCYATSVILALAPLLKADVLDRSRGIISDSKSGVSGAGKEPTAKTHFVSVADNLSAY
                     SVFSHRHVGEMIEQLELTAGELTFTPHLLPIPRGILSTLYVHLNRKMTAAEVELCFRS
                     FYAGKRWVRVFPTPKLPQVQFSLNTNYCDLGFCLADDGQRLVLISCIDNLLKGAAGQA
                     VQNMNLMYGWAEDEGLQ"
     gene            9198..9983
                     /gene="argB"
                     /locus_tag="VEJ00_06175"
     CDS             9198..9983
                     /gene="argB"
                     /locus_tag="VEJ00_06175"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HXX00780.1"
                     /translation="MTIVVKIGGAALEDAATLRKCARAIAELAQDGHRVAVVHGGGSA
                     LTRVLKQLGKQSEFVGGLRVTDAETRDVALMVLGGMVNKKLVAAIQAAGMPAVGFCGG
                     DGMSFRARKKSVRGNDLGFVGEICFAEPCWIEALWQQGGIPVLASLALGADGEYYNVN
                     ADEMAAACAAACHANALIFLTDVPGVKDADGAVIPWLSTKQAEDLAAGSIISGGMLPK
                     LAACKQALKQGVGRVRILPAAEADSLPQFYLTKIECGTEVTCV"
     gene            10103..11080
                     /gene="argF"
                     /locus_tag="VEJ00_06180"
     CDS             10103..11080
                     /gene="argF"
                     /locus_tag="VEJ00_06180"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXX00781.1"
                     /translation="MPDTFPGVFWCPDLISTRDLGPQGVEAVLHLAELMKSRPSVFQR
                     ALAERQMVMFFEKPSLRTRLTFEAAMTSLGGTAMFVDQTHSRIDARESLSDIAHNLER
                     WVDVIVLRTFAHETIEGMARFASVPVINALSDLEHPCQALADYLTLQERFGDLREITL
                     AYVGDGNNVAHSLLLTGACLGSSVRVATPQGYEPNAQIVGEARKIAKQTGARIELMAD
                     PQAAVAGVDAVYTDAWASMGQEEEAEKRARIFPPYQVNRALMAEAAPHAAFMHCLPAH
                     RSEEVTDDVIDSENSVVFEQAENRLHVQKAILYLLLGGAVRLPVRSAHA"
     gene            11073..12275
                     /locus_tag="VEJ00_06185"
     CDS             11073..12275
                     /locus_tag="VEJ00_06185"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HXX00782.1"
                     /translation="MPEKVVLAYSGGLDTSIIIPWLKENYSYDVIAMVGDVGQGDDIE
                     AVVEKAYATGACKVIVEDLREEFLTGYVFPALKAGAVYEHKYLLGTSLARPVIAKHQV
                     EVALREGATAVAHGCTGKGNDQVRFELTYQALAPELKIIAPWREWNLKSREDCLDYAE
                     QHGIPVAASREKIHSRDRNLWHVSHEGGELEDAGNAPLPSTWQMTNSPQEAPDKEEQV
                     SIGFERGVPISANGIEMDPVSLVELLNEIGARNAIGRVDLVENRFVGIKSRGCYETPG
                     GSLLIAAHRELEALCLERDVAHFKQHVGLKYAELVYFGLWFTPLREALDAFVDSTQKE
                     MTGSVTLSLYKGNVSVVSRQSANSLYRTDLSSFTMGDSYDQKDAAGFIRILGLPSRSR
                     ARARVEVA"
     gene            12275..13708
                     /gene="argH"
                     /locus_tag="VEJ00_06190"
     CDS             12275..13708
                     /gene="argH"
                     /locus_tag="VEJ00_06190"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXX00783.1"
                     /translation="MKMWAGRFREPLDAEFERWQRSFGFDRRLLQYELAASAAHARAL
                     KDAGVLSADELIGILQGLEQIGERTAASPAFLEDNEAEDVHHFVEKQLVTLVGDLGYK
                     LHSGRSRNEQIATDLRLYVRAAIDHLRQELAELCRVLVDRAEQAADAAMPAYTHLQKA
                     EPVLAGHWLLAYVEMFLRDANRLADCRKRLNACPLGSGAVAGATLPLDRGFMATTLGF
                     DAPTANSIDATSDRDFALEFVNASSLLAMHLSRWAEEMILLSSQEFGFLGLPEAYSTG
                     SSAMPQKKNPDLLELVRGKAGRAVGNATALLVTMKGLPLAYNKDLQETQQPVFGSADA
                     LLQMLPLVTGWMKAVEFHHDQMQKAAQSGFMNAWAAATYLVQRGVPSRLAHEKIGKAV
                     QMCLERSCELQDLPLDDLQSLSPVFAEDFYEALKLESVLAIHDVPGGTAPARVRQALA
                     AARTRILSLREEVHAQQTQAQETHAHA"
     gene            13695..>14068
                     /locus_tag="VEJ00_06195"
     CDS             13695..>14068
                     /locus_tag="VEJ00_06195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX00784.1"
                     /translation="MHTRKAILPDAEQIHELIAAYSGDGTLLPRTLAEICENVRDFVV
                     LEDEERIIGCGALHLYGTHLAEIRSITVAPWAQGKGGGGRLVKALLAEAKRHRVDCIC
                     LFTRKPDFFAAQGFSAAQREDIP"
BASE COUNT         2788 a         4028 c         4642 g         2610 t
ORIGIN      
        1 catattccgg tctgggtcgc aatcgcagag attgcggcta cggcgttcgt tgtgctttcg
       61 atcgtcgcga tcgtggttta cgcgccgttc tggattctcg gagggcttag caagaaacgc
      121 cggcgcccgg cggaacgaag catgagggtc tggccgttgc tggcggtgct gagcctgatc
      181 gtatttgtga ccatcttcat tgtgtccggc gatgatttga tcgagcgaat ggggaacctg
      241 accatctggt ctgcgggagt tttactttcc agtctcgcat ttgcggtcac gacgatcgta
      301 agtgcaatcg ccgtttaccg tgcgccggca gaaacagtcc gcaagaccgt gcgcagattt
      361 tcggcggttg ttactgtggc cctggtcatt gcgacgctct atctcgccta ttggggtgtg
      421 atcggcttgc ggacctgggc ttaggcgaat taccggttca ggccagaagg aagagtctct
      481 gggggctggg ctggatattc ggctatttgc agtcgggccg cgaggatgcc gccaatcgtc
      541 ttgatgtgcg gctcgcgcaa gagattttca tgatccagct ccatctcgtg cagttcgact
      601 ttactcagac agcgctgact ccatcccatt gcgcggtcat tgatgtagaa gaagggctgc
      661 ctggggcgct tgaagagcac aacgggagca cggaacttgt gcggcttgaa ttcgcggccc
      721 ggccaataga tctgccccca ggtcggccgg gacgattgtc gcggtgacga ctctttttgt
      781 cgccgtcggt cgagaacttc acgtcctgat ctcaaccaat ctctagccgg cagcttcccg
      841 gcgtggcgca ggcgtgtggc gtagtaatcg atcttccact tccacgggac catcgtgttc
      901 tgcagaggcc aagtatcgag gatggcgaag aatccgactt gctgtccgct gcgctcgagc
      961 tgaaggatca tttcttcgca aaccagcacg tcgtcgcaca tggcgatgag atgataagga
     1021 cccccggctt gcaccgagcg catcgcgctg atgtagtcct gcgcgagagc ttcgagctcc
     1081 gaacgcgtga ggggctgacc gttcggagcc ggatgcctgg cctgcagggt atacaagggt
     1141 tgccgcggac ccagatggcg cgccagggca gcgtagccga tggtttcgac tcccggaacc
     1201 accacagcga aaagcggcgc cctcccgttc tcgccgcgtt ggatgcaagt cacgacagag
     1261 tctggcgaag ccgcgccgcc tgacgcgatc aggctggcga gactttccac ggtggcggcg
     1321 tgcagcatcg ctgacaaggg aatctcgtga ccggtgttcc gcttgatttc ggaaagcagc
     1381 cgggcagcct gcaaggagtg gccgccgagt tcgaagaaat cgtctttgac gcccagtgtt
     1441 tgtgtgcgaa ggaccttgcg aaatattggg aggatagtgc tttcgagacc attgcgggca
     1501 tcgacgattt cgcgggcttc gccgcgctcg tccttcgggc ggggcagggc gcggcgatcg
     1561 acctttccgc tcgtggtcat gggcatctcc gtgaggacca cgaagcggga gggcagcatg
     1621 aactccggta acttttcctt gaggtgaacg cggacggcgt cgagcgggat gtccttaccc
     1681 ggaacgggca tgagatatgc gaccagccgc ttgtcgccgg ggatatcctc cctcaccgtg
     1741 accgcggcca actgcacgcc ggcacactcg ctgagcacgg cttcgacctc cccaagttca
     1801 atgcggaaac cgcgcagctt cacctgatcg tcgagtcggc cgaggaattc gatgttgcca
     1861 tcggggagaa agcgggccag gtcgcctgtc ttgtagaggc gcgcgtttgc attctggctg
     1921 aagggatcgg ggatgaattt ttccgtggtg agatcggggc ggttgaggta gccgcgggca
     1981 agctgaattc ccccgatgtg caactctccc gcgacgccga cgggcacagg ctgcagttgc
     2041 tcgttgagaa tgtaggcctg cgtgttggcg accggcttgc cgatgggaac gacgggccgg
     2101 gcgtagccgg gattgcattc ccagcaagta acatcaaccg cggcttcggt cgggccgtag
     2161 agattgtgca acttgccgtg gaagcgctcg aagaatcttg attgcagctc gagcgacaga
     2221 gcttcgccgc tcgcgaacac ctggcgtacc gaggtgcaac ggtcgagatc gtcggcctcg
     2281 aggaaaagcg ccagcatcga cgggacgaag tggagcgtcg tgatctgctg ttcctgaatc
     2341 agcttgacga ggtatgccgg atctttgtgc cctccggggc gggccatgac cagcgtcgcg
     2401 ccggtgatca gcggcagaaa gaattcccac acggagacgt cgaaactgta aggagtcttc
     2461 tgcaggatgc gatccgcctt tgtgacctgg tacgcatccc gcatccacag gagccggtta
     2521 acgatagcct cgtgaatgtt gagcacaccc ttgggcttgc cggtcgagcc tgaggtgtag
     2581 atgcaatagg cgaggttctt cccggccagt tctggcgatg ggttgtcggc attctcttgc
     2641 gtgagcgagg gccagtcgcg gtcgaggcaa atgacttctg cttctgataa aggcacgcgg
     2701 gcgagcaggt gcgcctgggt cagaacaact ggaggcttgg aatcctgcaa catcgttcgc
     2761 aggcgatcag caggatactc ggggtcgagc gggacatagg ctcctccggc cttgagagtg
     2821 gccaggagcg cgatgaccat ctcgagggag cgctcagcgc ataccgcaac cagcacgtca
     2881 ggaccgacgc ccagcttgcg caggcgatga gcaagctggt tggtgcgttg gttcagatcg
     2941 cgataggcga gttgctgaga gccgctgacg acagcgactg cgtcaggagt gcgctcgacc
     3001 tgctcctcca cgaggcggtg aagcggaatg tcacgcgggt agtcgactgc cgtctgattc
     3061 cagtcgacga cgatggtctg tctttcggcg tcgctcaggc ttggtgtggt gccagatgga
     3121 gaagcatcgg atgcagacac ggccctcggc gagttggaag aaagcgggta gccgagttcc
     3181 tccatggtcg ctccccacgc atgttcgatg gcgagcacag aggcctcggg cagtttgccc
     3241 tgccagccgc cggcccgggc ctcgccgacg aaaggaacat cctgacgggt gtctttcgtg
     3301 agttcccact cggcagaata ctttttctcc tggttgcgca tgttggcggc cgaactcaac
     3361 tcgactgcgc gtgcgattcg cgccggtgag gaatcaatct tgagcgcgga tgtaatccgg
     3421 accagctctt tctgcgggtc ggccagcagg tcttcgtact tgagcagcag gaagccgctc
     3481 tgttccttcc gcgcggcgcg ccagctgcga acgtgatctt gccaattgcc ccagtcgacc
     3541 aggaactcgc cggcaatgaa gcgcgggatg aattcctcga tgggatagcc gtcgggaatg
     3601 tttcttctct tgaggctgta cttgtaaagg gaaacagcga cgtcgcgggg atcgcggacc
     3661 aggtaaatcg tccttccgta tctggggtcg aagtactcgt gactcttcag aacgcggggg
     3721 cgggcgagcc tccgcaactg tcggtcggta aagaaataaa tctccgggat gcgcgactcg
     3781 acattggcaa aggtgacggg gtcgtcgggg cggagcagat tgccaatcag gaagcgcgtc
     3841 caggtgttgc ctgatctggg ataagacacg atgaagatat cgtcggggaa aacagtgaga
     3901 ttccgtcccg ccttgctgag tccgaacgcc cgtcgggcgc gatatttcag gcgattccag
     3961 atcatgggcg gcgggtctgc tccgggcgcg aagcggcgat ttcgcgggcc aggttatcga
     4021 tgcaggtcct gagcacagag tagcatcggc gagtgccgtc gaggctttca aagtacgggt
     4081 cgtggatctc gcgatcgcgc tgttcgcctt cggcgaaggc gctcaagaga aaaatcttgt
     4141 ctcgcgccgc cgggtagagc gtctgcagtt cggcgagatt ctcgaaatcc atggcgaaga
     4201 tagcgtcgga ggtggcaacg agttccttgg atacgggctg ggcgcggtgc tgatcgagcg
     4261 gcagcgacag ctcgcgcgaa acggcgagag cccatgggtg agcttcgcgg ccgggcacgg
     4321 cgtgcactcc agctgagtgg accgcgatgc cgttgatgcc ccgttccctc agaacgtgcc
     4381 taaacatggc ctcagccatg ggactgcgca tgaggttgcc atagcagacg aacaaaaacg
     4441 atgctgcgtt gcccgcaacc acgtgcgcgt tgccgctgtc gagatgaaaa tcatgcgtcc
     4501 gcaagctacg gtaggccgag cgagcctcgg gcgtgaggcg ggacagcgga tcggcaggcg
     4561 agtttcggcg ccggggcagg agccggcgcg acgcagactt aagagctgag gtgaggaaga
     4621 cacggattgc gccacccagc tctgcgaaga aaggcgccgg atcgcgccag gaccagagcg
     4681 cttctttcgt gaccggggaa aagtcggcca gagctgagag gagttccatg agttttgagc
     4741 ggcgaggagt gacctctcga ccgcctcggg agaggaggct ctggatgcgc tccacacaac
     4801 ccgggctcca ccgccaacgc aggccgacgg catagcgcgg gggaacgcaa ggcttctcac
     4861 cgtggagaag ctgccagtag tagaggggaa aatcgatgcc ggcgaggatg ggtagcgaga
     4921 cagatcccca gaagcgcccg ttcacctcca tgaggacgga caggccagtc gctgagtcaa
     4981 cccggaattc gaccatggcg acgccctccc actcgagggc ccgcagcagc gccattgagg
     5041 attcgagcag ttgcgggctg acactctcgg agacggcgac gacggcgacg cccccgctgg
     5101 ccggagcttc tttaagccgg cgatgctgaa aactcgcgac gcattcgccg ttgtgaatga
     5161 gaatttcaac tcccacaccg gcgccggggc agtattcctg cagcaacaca ttgccccagc
     5221 cgcgactgcg cagcgccagg gacaaatcgg ccaggctgct gaaatagaag acgcgcccgg
     5281 cggaggctga gccttttcgt tcaggcttta ccaccacggg aaagcgcagt tggggcgaga
     5341 tcgaatccag ctccgcggca gaagagatgc tgcgcgtgaa cggtactcgc aggccgcagc
     5401 gctgagcgat ctggagcgtg agcgacttgt tgagcacgcg ctcgacggtg gaaggcggcg
     5461 ggcaaccgac atgcagcaaa ggactgagta cgtcgtacag tcccgccagg gcggcgagcg
     5521 cgggatcgct cgctggcaga atcaagtcga atggctgctc tcgcaccagg ccgagcagag
     5581 cggcgctgaa cgcggcggga tcatcctgcg gcggcggcag ccggtgaaac tggcggacgg
     5641 cacgagagcg gatggcaggg tcttcgggtt gtaaagaggc aatgtcgacg ggaatcccgc
     5701 cacgattcaa cgagcgcgcg atcgacaggc tgatacgcgg tgccgcgcct aacacaagga
     5761 ccggccggtg tttggaaatg ctagtcatca aacattgacg tggcgccggg acaggcagtt
     5821 catggaagtc cggcgcgatc gatcaagctt cttgccgcga ggtctcggcc aactcgatgc
     5881 cgatctccgc gtcccgctca tcgtaggata gtcccctgac ctgccaggcc ttgcgcgtcc
     5941 agatcgccca ggccagcccg attccgtaga cgcccagcgc aatcaccgtc tgctccgcca
     6001 gcggcaccag atgatgcgga acgaaccagc ggcgcagcag caacagcgcg cccaccaggg
     6061 gaacggtcag gcccagcggc agcagaaagg aatgtctcag gtacgagagc aaccgcagct
     6121 tcagcagacg gcagaggtga tgcggcagga aaaacaagac ggtacaggaa agcggaatgg
     6181 ccgtgcccag cgcgtcgccc acgatgccgt acttgcgcac caggataatg ctcagcacta
     6241 aattggcgat tccttcggcg agcgtgactt tcgcgagcat ctgatgtttg cccatgccga
     6301 acagaactcg cgaagaggcg gcctgcatga tcatcagcgt ggacggaatg agcaggatca
     6361 gcaggatcgg atagctggcc gccacgtaac gcgcgcccat ccaggcctcg atcaccgatt
     6421 tgccgagcag aaccaggatc gcggtgatgg ggaaaatgac cagcgcgcac atgcggtttc
     6481 ccgcgacgaa gatctgccgg aggcgctgca agtctcccag agcatccgac tggctcgaca
     6541 tcggcacgaa gatctgcgcc atgctctgca caacctcagt cgcgtaatcg accaggcggg
     6601 cgccgatggt gaaataagtt actgccgcag ccgacaggaa ggtgccgatc accagggcgt
     6661 cggtcttgaa acgcaggcgt ccggccacga tgatcacaaa cgtcgtcccg ctgtagctgg
     6721 cgatctggct gagcgtggag cgtttcacgt agccgattcc cagccgcaag ggcaggtggc
     6781 gaaagactgc ggcgccgttg atcagcccgc ccattagcgg catcgacacc gtaatcagtg
     6841 cgacggtgag gagtccgcgg ccgttgcgca aggcgatcac gatgagcagg gcgcgaagca
     6901 gggtagccgt gatggtagtg aaattcagca ggtagaaacg ctgcaggcct tccagaatgc
     6961 cgctgaacac agcgaaagga aagccgagcg agacggcggc gcccacgatc aggagcagcc
     7021 agcgcgcagt gttgacgaac tcgggaggaa ttttgaagat cgcattcacg tatccggctg
     7081 caaccagggt gatgagcatg gtcacggcgc caatgcagct gtagctggct agcgcggtac
     7141 tcaccagccg gtccagttcg tcgcgttcgc cggtggcggc gaacttggcg acgtagcgaa
     7201 cgatcgacga ccggataccc aggtcgaaca gcccgtagta tcccgtaacg gaaaagatca
     7261 gaatccagag accgaaggcg gtgtctccga ggcggtgcag gatgtaggga gaaaggaaga
     7321 ttcccaccag gacgttcacg cccagcgcga accagctcga tcccacgttt cttaggattt
     7381 ggcccttgtc gaacttcatg aaccccgctg caggtgcttg ttgtggctcg cggaagccgg
     7441 gaaccgcccg cgggacgccc taaatagagt agtggaatcg ccgggggcag gcaacgggcg
     7501 cgaaggccac cgcgcgcgtg tcccaaaggc catgaccgcc ctttcctggc cgcccgcgac
     7561 tgcaaacggg cagtctttgg aacacagtaa ccagcggagc cccgcccgcg attttcgctt
     7621 gaccggcaag tttcccgccg gtaatatacg tcaataatta ttcattctga tgaataaata
     7681 tccattatga ataaaaccct gcgtcaacgt gccgttctcg ataccttgag acacggtcac
     7741 atctcgagcc aggaggagct gcagcgcgtg ctgcgcaggc gcggctttcg cgtggggcag
     7801 gctactctgt cgcgcgacat ccgggatctg aatttgagca agaccgcgca cggctacgcc
     7861 ctgccccagg gcgaaggctc cgccggagtt gcattgccgc cggtgtcacg gctggttcgc
     7921 gaatttgtac tggatgtgcg ttcggcccag aaccagctgg tgatcaagac catcgtgggc
     7981 agcgcgcagc ccgtggcggc tgcgctcgat gaggccgatt ggcccgaggt cgtgggcacg
     8041 atcgccggcg acgacaccat cctgattgtc tgcccggata aggaagaagc cggcaagctg
     8101 gcgggccgta tcgaggagat gctgagctga tttgacacgg aagatgacgg ccaagctcaa
     8161 aaccgcggtg ctgggcgcga ccggatattc cgggctggag ctcacccgca ttctgctgcg
     8221 ccacccgcag gtggaaaagc cggttctgct tcggcgttcg agcgacaacg ggacgcgcga
     8281 tctggcagaa gttttccccg cgctctcggg caatggaggt tatccgctgc aaccgctttc
     8341 gtggccgacg ttgaagcaac agggagtgga gctgctgttt ctggcgacac ctcacgaagc
     8401 ctcgcgcgcg ctggtgcctg aggcaatcgc tcatgggctg cgtgtggttg atctcagcgg
     8461 ggcgtggcgg ctgaagcagg aacagcatcg cgcgatctac agctttcagg acgcaaaccc
     8521 ggtcacagcc gccgagctca ccgccaaagc gatctacgga ctgcccgagc tgaacgcgga
     8581 ccgtctggcg aatgcgacgc tggtggcgaa tcccggctgc tatgccacgt cggtgattct
     8641 cgccctggca ccgctgctca aggcggatgt gctcgatagg agccgcggaa ttatctcgga
     8701 ttcgaaatct ggggtttccg gcgcgggaaa agaacccaca gccaagactc attttgtgtc
     8761 ggtggccgac aatctgtccg cctattcggt gttcagccac cggcatgtcg gcgagatgat
     8821 cgaacagctg gaactgacgg ccggggaatt gacgttcacc ccgcacctgc tgcctattcc
     8881 gcgcgggatt ctttcgacgc tctatgtaca cttgaaccgg aagatgacag cggcagaagt
     8941 ggagttgtgt ttccgcagtt tctacgccgg caagcgctgg gtgcgcgtgt ttccgacgcc
     9001 caaacttcct caggtgcaat tttcgctcaa caccaactac tgcgatctgg gattttgtct
     9061 ggcagacgac ggacagcgtc tggttctgat ctcgtgcatc gacaacctgc tcaagggcgc
     9121 ggccgggcag gcggtgcaga acatgaatct gatgtacggc tgggcggagg acgaggggct
     9181 gcaatgacgg gcttgcaatg acaatcgtgg tcaaaatcgg aggggctgcg ctggaggatg
     9241 cggccacgct gcgcaaatgc gcccgagcca tcgcggaact ggcgcaggat ggccatcgcg
     9301 tggccgtggt tcacggcggc ggcagcgccc tgacgcgcgt gctgaagcaa ctgggaaagc
     9361 agagcgaatt cgtcggcggc ctgcgggtaa ccgatgccga gactcgcgac gtggcgctga
     9421 tggtgctggg cgggatggtc aacaagaagc tggtagctgc cattcaagct gcaggaatgc
     9481 cggcggtggg cttctgcggc ggcgacggca tgagcttccg cgcccgcaag aaatccgtac
     9541 ggggtaacga tctgggattc gtcggcgaga tctgtttcgc ggagccgtgc tggattgagg
     9601 cgttgtggca gcagggaggc atcccggtcc tggcgtcgct ggctctgggc gccgacggcg
     9661 agtactacaa cgtcaatgcc gacgagatgg ctgcggcctg cgctgccgcg tgccacgcca
     9721 acgcgctgat cttcctcacc gacgtgccgg gggtgaaaga cgccgacggc gcggtgattc
     9781 cctggctgag cacgaagcaa gcggaagatc tggcggcggg atcgatcatc agcggaggca
     9841 tgttgccgaa gctggcggca tgcaagcagg cgctgaagca gggtgtgggg cgcgtcagga
     9901 ttctgcctgc cgccgaagcg gattccttgc cgcagtttta tctcaccaag atcgaatgtg
     9961 gcacggaagt gacttgcgtc tgacgagatt ccccacttct cgtgcagata acgcgcgaga
    10021 aatggggcac ccatttgagg gtatttctgc aggagaaaga ggaactacgt tgcatagtcc
    10081 attgcaaagc agagttccgg cggtgccgga tacctttccc ggcgtcttct ggtgccctga
    10141 cttgatttcg acccgcgatc tcggcccgca gggtgtggaa gcggtgctgc atctggcgga
    10201 gctaatgaag tcgcggccgt cggtctttca gcgggcactc gccgagcgcc agatggtgat
    10261 gttcttcgag aagccgtcgc tgcgcacgcg gctcacgttt gaagccgcca tgacgagcct
    10321 gggcgggacg gcgatgttcg tcgatcaaac gcacagccgg atcgacgcac gcgagtcgct
    10381 gagcgacatc gcccacaacc tcgaacgctg ggtcgatgtg atcgtgctgc gcacgttcgc
    10441 gcacgaaacg atcgaaggga tggcgcggtt cgcctcggtt ccggtgatca acgcgcttag
    10501 cgatctggag catccctgcc aggcgctggc cgactatctc acgctgcagg agcggttcgg
    10561 cgatctccgt gaaatcacgc tcgcttacgt cggagatggc aacaacgtcg cgcactcgct
    10621 cttgcttact ggcgcctgtc tgggatcgtc cgtccgcgtc gcgacgccac aaggctacga
    10681 gccgaatgcg cagattgttg gcgaggcgcg aaagatcgcc aagcagacgg gtgctcgcat
    10741 cgagttgatg gcagacccgc aagctgccgt cgccggagtc gacgccgtct acaccgacgc
    10801 ctgggccagc atgggacagg aagaggaagc ggaaaagcgt gcccgcatct ttccgccgta
    10861 tcaggtgaac cgggcactga tggcggaagc ggcgccgcat gcggcattca tgcattgcct
    10921 gcccgcccat cgctcggagg aagtcaccga cgatgtcatt gactcggaaa actcggtggt
    10981 cttcgagcag gccgaaaacc ggctgcacgt gcaaaaagca attctgtatc tattgctggg
    11041 cggcgcggtg cgcctgcccg tgaggagcgc tcatgcctga gaaggttgtt ctcgcgtatt
    11101 ccggcggact ggacacttcg atcatcatcc cgtggctcaa ggaaaactat tcgtatgatg
    11161 tcatcgccat ggtgggcgac gtgggccagg gcgacgacat tgaagcagtg gttgaaaagg
    11221 cctacgccac cggcgcttgc aaggtgatcg tcgaggacct tcgtgaggaa ttcctcacgg
    11281 gctacgtctt tcctgcgctg aaagccggag ccgtgtacga gcacaaatac ttgcttggga
    11341 cttccttggc ccgtccggtg attgcgaagc accaggtgga agtcgcgcta cgggaagggg
    11401 cgactgctgt ggcccacgga tgcacgggaa agggcaacga ccaggttcga tttgagctga
    11461 cctaccaggc tctggctccc gagctgaaaa tcatcgctcc gtggcgggag tggaatctga
    11521 agtcgcgcga agactgcctc gactacgccg agcagcacgg aattccggtc gccgccagcc
    11581 gcgagaagat tcacagccgc gaccgcaatc tgtggcacgt cagtcacgag ggcggcgaac
    11641 tggaagacgc cggcaacgcg ccattgccga gcacctggca gatgaccaat tcgccgcagg
    11701 aagctccgga caaagaagag caagtcagca tcggatttga gcggggcgtt ccaatctctg
    11761 ccaacgggat cgagatggat cccgtgtcgc tggtggaact gctgaacgaa atcggggcgc
    11821 gcaacgccat cggccgcgtt gatctggtgg aaaaccgttt tgtcgggatc aagtcgcggg
    11881 ggtgctatga aactcccggg ggctcgctgt tgattgcagc gcatcgcgaa ctggaggcgc
    11941 tctgcctgga gcgcgatgtt gcgcacttca agcagcacgt cgggttgaag tacgccgagc
    12001 ttgtctactt cggcctgtgg tttacaccgc tgcgcgaagc gcttgacgct tttgtcgaca
    12061 gcacgcagaa ggaaatgacg ggcagcgtga cgctctcgct gtacaaaggg aacgtttccg
    12121 tggtcagccg ccagtcggcc aattcgctct atcgcacaga tctgtcgtca ttcacgatgg
    12181 gcgacagcta cgatcagaag gacgcggcgg gattcatacg cattctcggg ctgccctcgc
    12241 gctcgcgcgc ccgggcgcgc gtggaggtcg cgtgatgaag atgtgggcgg gacgcttccg
    12301 cgagcctctc gatgcagagt tcgagcgctg gcagcggtcg ttcggcttcg atcgcaggct
    12361 gctgcaatac gagctggcgg ccagcgccgc ccatgcgcgc gcgctcaagg acgccggggt
    12421 tctgtctgcc gacgaactga tcggcatcct gcaggggttg gagcagattg gcgagcgaac
    12481 cgccgcatcc ccggcgtttc tggaggacaa cgaagcggaa gatgttcatc actttgttga
    12541 gaagcagttg gtaacgctgg tcggagatct gggatacaag ctgcacagcg gccgcagtcg
    12601 caacgagcag atcgctacgg atctgcggct gtacgtgcgg gcggcgatcg atcatctgcg
    12661 gcaggaactt gccgaactct gcagggtgct ggtagatcgc gccgaacagg cggccgatgc
    12721 cgcgatgccc gcctataccc acttgcaaaa agccgagccg gttctcgccg gccactggct
    12781 cctggcttac gtcgagatgt tcttgcgcga tgccaaccgg ctcgcggatt gccgcaagcg
    12841 gctgaatgcg tgcccgctgg gctcgggagc ggtagccgga gcgacgctgc cgcttgatcg
    12901 tgggttcatg gccaccacgc ttggattcga cgctcccacg gcgaacagca tcgatgccac
    12961 cagcgaccgc gactttgcgc tggagttcgt aaacgcatcg tcactgctcg ccatgcattt
    13021 gagccgctgg gcggaggaaa tgatcctgct ctcgtcgcag gagtttggat ttctggggct
    13081 gcccgaagcc tattcgaccg gtagcagcgc gatgccacag aagaagaatc ccgatctgct
    13141 cgagctggtg cggggcaaag caggacgagc ggttgggaat gccacggcgc tgctggtcac
    13201 aatgaagggg ctgcctctcg cttacaacaa agatcttcag gaaacgcagc agccggtctt
    13261 tggttcggca gacgctttgt tgcagatgtt gccgctggtg accggatgga tgaaagccgt
    13321 ggaatttcat cacgatcaaa tgcagaaagc cgcgcagtca ggattcatga acgcctgggc
    13381 tgcggcgacg tatctggtgc agcggggcgt tccgtcccga ctggcgcatg aaaaaatcgg
    13441 caaggcggtg cagatgtgcc tggagcggag ttgcgaactt caggatctgc ctcttgacga
    13501 cctgcaatct ctcagtccgg tattcgctga ggatttctac gaagcgctga agctggagtc
    13561 ggtactggcg atccacgacg ttccgggagg cacggccccg gcacgcgtgc gccaggctct
    13621 tgctgccgcg aggacgagaa ttttgtctct gcgtgaggag gtccatgctc agcagaccca
    13681 ggctcaggaa acccatgcac acgcgtaaag ccatcctgcc cgacgccgag cagattcacg
    13741 agctcatcgc agcttactcc ggcgatggca cgctgttgcc gcgcacgctg gccgagattt
    13801 gcgagaacgt gcgggacttt gtcgttcttg aagatgaaga gcgcatcatc ggctgcggcg
    13861 cgcttcacct gtatggcaca cacctcgccg aaattcgctc gattacggtc gcgccgtggg
    13921 cgcagggcaa gggcggcggt gggcggctgg tgaaggcgct cctggctgag gcgaagcgcc
    13981 atcgcgtgga ttgcatttgt cttttcaccc gcaagcccga cttcttcgcc gcgcaaggct
    14041 tctcggctgc gcagcgcgag gatatccc
//
LOCUS       DASPUL010000246         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_317110, whole genome shotgun sequence.
ACCESSION   DASPUL010000246 DASPUL010000000
VERSION     DASPUL010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317110"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VEJ00_06200"
     CDS             <1..979
                     /locus_tag="VEJ00_06200"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HXX00785.1"
                     /translation="VREARFIRNPDGTRDGGVHDLYTIAGRSDAVGCMLGQPDFATEL
                     ANNNVIFRTPTPVFGLGLVEATPDLILQQNLASTQAARAAMRIGGSFNTSGNDGTITR
                     FGWKAQNKSLLIFAGEAYNVEQGVSNEVFPNERSAVGGCVFNSSPEDASNMLNQNSSS
                     PNFGTPYGTVSEMSSDVANFAVFMRLSAPPTPAPPTTSTRNGAALFNSVGCALCHSPT
                     LTTGPSDFTGMSNITYHPYSDFALHHMGWNLADGIHQGNAGADEFRTAPLWGLGQRLF
                     FLHDGRTSDLIEAINAHGDRDSEANRVVRRFRTLNPSQMQDLLNFLRSL"
     gene            complement(1105..>2027)
                     /locus_tag="VEJ00_06205"
     CDS             complement(1105..>2027)
                     /locus_tag="VEJ00_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HXX00786.1"
                     /translation="RYPVVILLHGMNQDADDMERLTQFDALADKDGIVAVYPFALHGR
                     WNTGVHPQERRPMGMGPGGGHHRGGYGGGYPGGGGYPGGGGGYPGGGQPRSRQPDEER
                     RPVPADDIAFFNQMLDQLETKLSIDSSRIYVGGLSEGGLMSMRVGCAMADRVAAVAAV
                     GAAMPKTMICLPSRPVPVAMINGTSDPVVPYGGGTEHNLNLGILSAEDSAKAWAKIDR
                     CEEKPAHDKLPPREKGGMETKVDTYTACQQGAQVVLYSVKGAGNTWPGGEQYEAENTV
                     GKTSHDLNANETLWSFLVTKKAAAKNDSGK"
BASE COUNT          373 a          664 c          578 g          412 t
ORIGIN      
        1 cgtgcgcgaa gcccgtttca tcaggaatcc tgacggcacg cgcgatgggg gcgtgcacga
       61 tctctacacg atcgccggcc ggtccgatgc ggtgggttgc atgctcgggc agcccgactt
      121 tgccaccgaa cttgccaaca acaacgtgat cttccgcacc cccacaccgg tgttcggact
      181 tggactggtg gaagccacgc cagacctgat tctgcagcag aatctcgcgt ccacccaggc
      241 ggcacgagca gccatgagga ttgggggaag cttcaatacc agcggcaatg acggcaccat
      301 tacccgtttt ggctggaagg cccagaacaa atccctcctg atcttcgctg gcgaggccta
      361 caacgtcgag cagggagttt cgaacgaggt gtttcccaac gagcgcagcg ccgtgggcgg
      421 ttgcgtgttc aacagctcgc ccgaagatgc ctcgaacatg ctcaaccaga actccagcag
      481 cccgaacttc ggcactccat atggcacggt ctcggagatg tcgtccgatg tcgcgaactt
      541 tgcggttttc atgcgtctgt cagcgccgcc cacacccgcg ccgccgacaa cgtcaacccg
      601 gaacggagcc gccctgttca attcggtcgg ctgcgctctc tgtcactcgc cgacactgac
      661 tacggggccg tcggacttca ccggtatgtc gaacatcaca taccatccct actcggactt
      721 cgctttgcat cacatgggct ggaacctggc ggatggcatc catcagggga acgccggggc
      781 ggacgaattc cgcaccgctc cgctttgggg actgggccaa cggctgttct tcctgcacga
      841 tggccgtacc agcgacctga tcgaagcgat caacgctcac ggcgaccggg actcggaagc
      901 caaccgggta gttcgcaggt tccgcactct gaatccgtcg cagatgcagg atcttctgaa
      961 ctttctgcgc tcgttgtagg agtgaagtga agagataaac cggtgagccg agctggctcg
     1021 ggctagctcg ttaaacaatc gcggagagcc ctgcctctcc gcgattcgct tttcaaactg
     1081 gacaatcgga gggcgaagta cctgttactt cccgctgtcg ttcttcgcag ccgctttctt
     1141 cgtgaccagg aagctccaca acgtctcatt ggcattcaaa tcgtggctgg tcttgccgac
     1201 ggtgttctcg gcctcgtatt gttcccctcc gggccaggta tttcccgctc cctttacgct
     1261 gtagagcacg acctgtgcgc cctgctgaca agcggtgtag gtatcgactt tcgtctccat
     1321 gccgcctttc tcgcggggcg gaagcttgtc gtgcgccggt ttttcctcac agcgatcgat
     1381 cttggcccag gctttggcac tgtcctccgc ggaaagaatc cccagattca gattgtgctc
     1441 agtgccgccg ccataaggaa cgacgggatc ggatgttcca ttgatcatgg ccaccggcac
     1501 cgggcgcgag ggcaggcaga tcatggtctt cggcatggct gctcccacag ccgcaaccgc
     1561 ggcaacacgg tccgccatgg cgcatcccac gcgcatgctc atgagtccgc cttcggaaag
     1621 ccctccgacg tagatgcgcg aggaatcaat cgacaatttg gtttcgagct ggtccagcat
     1681 ctgattgaag aacgcgatat cgtccgccgg aaccggcctt cgctcctcgt ctggctgtcg
     1741 gcttctgggt tggccgccgc ccgggtagcc gccacctcct ccgggataac caccccctcc
     1801 ggggtaaccg cctccgtagc cgccgcgatg gtgtccgcca ccgggcccca tgcccatcgg
     1861 ccgccgctcc tgcggatgca ctccggtatt ccagcgcccg tgtaaggcga agggatagac
     1921 agcgactatg ccgtccttgt cggccagcgc gtcaaattgc gtcagccgct ccatgtcgtc
     1981 ggcatcctgg ttcatcccat gcagcagaat caccaccgga tagcggc
//
LOCUS       DASPUL010000247         4467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_591987, whole genome shotgun sequence.
ACCESSION   DASPUL010000247 DASPUL010000000
VERSION     DASPUL010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591987"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /gene="obgE"
                     /locus_tag="VEJ00_06210"
     CDS             complement(<1..761)
                     /gene="obgE"
                     /locus_tag="VEJ00_06210"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXX00787.1"
                     /translation="MFIDEAKIRVKAGDGGNGCMAFRREKFVPRGGPSGGDGGKGGDV
                     VMESSERHNTLVHFRFNPEYKAERGRHGEGANKTGRDGGEVVLKVPVGTIVYDDETGE
                     KVFDFSGPDQHIVIARGGRGGRGNARFATSVHQAPREHEAGRPGEERLYRLELKLLAD
                     VGLVGYPNVGKSTLISRISAARPKIADYPFTTLEPNLGVVAVGDAKDEISFVVADIPG
                     LIEGAHTGAGLGTQFLRHIERTRLLVHLVDVSDSSG"
     gene            complement(895..1158)
                     /gene="rpmA"
                     /locus_tag="VEJ00_06215"
     CDS             complement(895..1158)
                     /gene="rpmA"
                     /locus_tag="VEJ00_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXX00788.1"
                     /translation="MAHKKGQGTSRNGRDSNSQRLGFKAFGGQVVPGGTIIVRQRGTR
                     VKPGLNVGRGKDDTLFAKITGRIKFMDKGSMGRFVMVEPVEVK"
     gene            complement(1270..1743)
                     /gene="rplU"
                     /locus_tag="VEJ00_06220"
     CDS             complement(1270..1743)
                     /gene="rplU"
                     /locus_tag="VEJ00_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXX00789.1"
                     /translation="MYAVIRAGGKQYRVAPGDVIRVEKLGTGTDGQVEFPVLAVSGEQ
                     GQVSQQSDARVVGHVVEEGRGAKVIVFHYKRKKQYKKLRGHRQAFTAVRIAEIAFGGQ
                     SFKAPEQPKKEAKPKKVKAAEAEAPEAEAKAAKGAAKKKATAPKKAAKKTSGKKK"
     gene            complement(2079..3239)
                     /locus_tag="VEJ00_06225"
     CDS             complement(2079..3239)
                     /locus_tag="VEJ00_06225"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HXX00790.1"
                     /translation="MSPKIFLGVMLLVIVAGLVVVDQRQARAGEVTGASGYKVLDPIR
                     HGNLMVFPVVATKSYPTGEFLTLDEGLRSGEVVVTEAGQVQGLIRRHGVPLLRHDGAE
                     VNRLVLVNNSKRPLLLLAGEIVTGGKQDRVIGKDRIVPPESDPVDLNVFCVEPGRWVA
                     TSEHFGASEAMYGNAASGVAGGAMHSPPPMAMMAQPSVRAKAMADKDQRQVWDAVRVQ
                     NEAVAVELVPAAPAASAEVARTSSYAKVMENEDVKKQVDAVAKPIEQNYQSLIGQLRD
                     RHAVGVVVAVNGRIIWADIFASTDLLAKYWPKLVRSYASEAVVTRAKDVDVSVSRAQA
                     FLEDMEGRRETIESEPGIYRHTEVTGDGFKAFSLTSLLPKTGFDVHVAKMAE"
     gene            3513..4436
                     /locus_tag="VEJ00_06230"
     CDS             3513..4436
                     /locus_tag="VEJ00_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXX00791.1"
                     /translation="MSPTQPISAVQSVVSDLLGELHAKSGCDKIGLTRESFAAILREV
                     GTKFATVATSEDEIRAFFVSLRVDELALARACADGNNSAWEIFLTRYREKLYLSALRI
                     AREDSAARELADTLYADLYGTTTREGQRVSKLSSYTGRGSLEGWLRTVLSQEYVNRYR
                     RTKRLVSLDEESEEGAQFQAPDPEPAAPADPRLAQATDEALAFLCGEDRLILSAYYLD
                     GRTLAEIARMLGVHESTISRKLDKLAKALRKQILTALTRRGMSRRQAEEALEADVRDL
                     QLDIRRSLTQEPPPSAFSEKRVEARVRDGPD"
BASE COUNT          817 a         1579 c         1194 g          877 t
ORIGIN      
        1 ccgctcgagt ccgaaacgtc tacaagatgc accagcagtc gcgtacgctc gatgtgccgc
       61 agaaactggg tgcccagccc ggccccggtg tgcgcccctt cgatcagccc aggaatatcg
      121 gccacgacga agctgatttc atctttggcg tcgccgaccg ccaccacacc gagattcggc
      181 tcgagcgtcg taaacggata gtcggcaatt ttgggccgcg ccgcagaaat acgcgaaatc
      241 agcgtcgact tgcccacatt cggatatccc accagcccga catcagcgag cagcttgagt
      301 tccagccggt agagccgctc ctcgcccggc cgtcccgcct cgtgctcgcg cggcgcctga
      361 tgcaccgacg tggcgaagcg cgcattcccg cgcccgccgc ggcctccacg cgcgattacg
      421 atgtgctggt cggggccgga gaaatcgaac actttctcgc cagtctcgtc gtcgtagacg
      481 atcgtcccga ccggaacctt cagcacgacc tcgccgccgt cgcggccggt cttgtttgcg
      541 ccctcgccgt gacgcccgcg ttcagctttg tattcgggat tgaagcggaa gtgtacgagc
      601 gtgttgtgcc gctcgctgga ttccatgacg acgtcgccgc ccttgccccc atcgccgcct
      661 gatggcccgc cgcgcggaac gaacttctcc cgccggaagg ccatgcagcc gttgccaccg
      721 tcgccagcct ttacgcggat tttggcttca tcgatgaaca tgggaaagca ggaatgcttc
      781 tgcgcgcttg tggctcgcga ccagaagcta aaaagcctcg tcccgaaggc gaggctgggt
      841 taagaacgtg tggcgcgggc gctcccgccc gcgactctct ccatgagcga tgctctactt
      901 cacttccacc ggctcgacca tcacgaaccg ccccatcgag cccttgtcca tgaacttgat
      961 gcggcccgtg atcttggcga agagggtgtc gtctttgccg cggccaacgt tgaggcccgg
     1021 tttcacgcga gtgccgcgct gccgcacgat gatcgttccg ccaggtacga cttgcccgcc
     1081 gaatgccttg aatcccagcc gctgcgaatt cgaatcgcgg ccatttcgtg aagtgccctg
     1141 ccctttttta tgtgccatag aaaccagttc tcagtggcca gttgccaggt gtcagttaga
     1201 accttgttac gattgcgctg ggtgccccat ttctcgcgcg gtctttgcgc gagaagtggg
     1261 gccacgcgtt cacttcttct tcccgctcgt cttcttggca gccttcttcg gagcagtagc
     1321 cttcttcttg gccgcgcctt tcgctgcctt cgcttccgct tcaggtgctt ccgcctcggc
     1381 agccttcacc ttctttggct tcgcttcctt cttgggctgt tccggagcct tgaagctctg
     1441 tccgccaaaa gcgatctcgg cgatccggac cgcggtaaac gcctgccgat gtccgcgcag
     1501 cttcttgtat tgcttcttgc gcttgtaatg aaagacgatg accttcgcgc cccggccttc
     1561 ctccacgacg tggccgacca cgcgagcgtc agactgctgc gacacctggc cctgctcgcc
     1621 cgaaaccgcc agcaccggga actctacctg cccgtcggtg ccggtgccca gcttctccac
     1681 tcgaatcaca tcgcccggcg ccacccggta ctgcttccca ccggcgcgga taaccgcgta
     1741 catacctctg cctccgtcaa gatctcatgc gattcagccc gaaaactggg tgctcgagcc
     1801 gaacgctacg gtcaaacccc ggacgcacac cgccgcgatc cacgggcctg acgaatttcc
     1861 tgtgcttgct gtgttttccg ccccggcgct tcggcagcca ggacaggcgt ccccagaacg
     1921 ggtcggcgag tacaggcaaa cccagttatt ttatcgtgat gagaagaaat cggtcaaaca
     1981 agcagggatc aggggccagg ggtcagaggt cggcagggga ttgatccgtc gactgatccg
     2041 tctggcccct gacccctgat ccctgacccc tgcgccctct actccgccat cttcgcaacg
     2101 tgcacatcga accccgtctt cggcagcaac gaggtcagcg agaacgcctt gaatccatcg
     2161 ccggtgacct cggtgtggcg atagattccc ggctcgctct cgatcgtctc ccggcgtcct
     2221 tccatgtcct cgaggaaggc ctgcgcgcgg ctcacgctca cgtccacgtc tttcgcccgc
     2281 gtcaccaccg cctccgacgc ataggagcgc accaacttgg gccagtactt tgccagcaga
     2341 tccgtgctgg cgaatatatc ggcccagatg atgcgcccgt tcaccgctac caccaccccc
     2401 accgcatgcc ggtcgcgcag ctgcccgatc aggctctgat agttctgttc gattggcttg
     2461 gccactgcgt ccacctgctt tttgacgtct tcgttctcca tgactttggc gtacgaactg
     2521 gtgcgtgcca cttccgccga cgctgctggc gccgcgggca cgagctcgac ggccactgcc
     2581 tcgttctgca ccctgaccgc atcccacacc tgacgttgat ccttgtcggc catcgccttg
     2641 gcccgcacgg atggctgcgc catcattgcc attggcggtg gagaatgcat cgcgccaccc
     2701 gcaacaccgc tcgccgcatt tccatacatc gcctccgaag ccccgaaatg ctcgctggtc
     2761 gcgacccatc gtcccggctc cacgcagaac acattcaaat ccacgggatc gctctccgga
     2821 ggaacgatgc ggtccttgcc gatcacgcgg tcctgtttgc ctccggtgac gatctcgccc
     2881 gccagcaaca gcagtggccg tttcgaattg ttgaccagca ccagccggtt cacctccgcc
     2941 ccatcgtgcc gcaacaaagg cacgccatgc ctccgaatca ggccctgcac ctggcccgct
     3001 tctgtcacca cgacttctcc cgaccgcagc ccctcatcca gggtcagaaa ctcgccggtc
     3061 ggataacttt tcgttgccac caccggaaac accatcagat tcccatgccg gatcgggtcc
     3121 agcaccttat accctgatgc ccccgttacc tccccagccc gcgcctgccg ctgatcgacg
     3181 accaccagac cagccacaat caccagcaac atcacgccta agaaaatctt tggactcata
     3241 agccgcccct ccgggcgtac gtatagagac acctcagaga caccccacgt tccgcgtccc
     3301 cttttccgaa cgtggggtct cgtgttgcaa gcgccacact cgcaacgcta catcgccttc
     3361 ggaaggagaa cgaaaatatc cgcttccctt gcggccaaaa ttcacgctcc gcacgtctaa
     3421 taaattggta gcatcaaggt gaatcgcgac cgtagttgcc aaaacgtgga tgaatccgaa
     3481 ctcgccgaag gataaatagc gccagcccgc gcatgagccc gacacaaccc atctccgccg
     3541 tgcagtccgt cgtcagcgac ctgctggggg agttgcacgc gaaaagcggc tgcgacaaga
     3601 tcggactgac gcgcgaatct ttcgctgcaa tcctccgcga agtcggaaca aaattcgcaa
     3661 ccgtcgcgac ttcagaagat gaaatccggg cgtttttcgt cagcctccgc gtcgacgaac
     3721 ttgctctcgc acgcgcctgc gccgacggca acaactccgc ctgggagatt ttcttgacgc
     3781 gttaccgcga gaagctgtat ctctcggcgc ttcgtatcgc ccgcgaagat tccgccgccc
     3841 gcgaactcgc cgacaccctc tacgccgatc tctacggcac caccacacgc gaaggccagc
     3901 gcgtctcgaa actttcgtcc tacactggcc gcggatcgct cgagggctgg ctgcgcaccg
     3961 ttctgtcgca ggaatacgtg aaccgctatc gccgcacgaa acgcctggtc agcctcgacg
     4021 aggagtccga agaaggcgcc caattccagg ctcccgatcc cgagcccgcc gcacccgccg
     4081 atccgcgtct ggcccaggct accgacgaag ccctcgcctt tctttgcggc gaggaccgcc
     4141 tgatcctgag cgcgtattac ctcgatgggc gtacgctggc cgagatagcc cggatgttgg
     4201 gtgtgcacga atcgaccatc agccgtaaac tcgacaagct ggctaaggcc ttgcgaaaac
     4261 agatacttac ggccctgacc cgccgcggta tgtcgcgccg ccaggccgaa gaagcactcg
     4321 aagccgacgt tcgcgacctg cagctcgata ttcgtcgtag tctcacgcaa gaacctccac
     4381 cctctgcgtt ctctgagaag agagtagagg cacgggtccg cgacgggccc gattagatag
     4441 atggagcggc ggacgcctcg tccgccg
//
LOCUS       DASPUL010000248         4842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_317522, whole genome shotgun sequence.
ACCESSION   DASPUL010000248 DASPUL010000000
VERSION     DASPUL010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317522"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VEJ00_06235"
     CDS             complement(<1..182)
                     /locus_tag="VEJ00_06235"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXX00792.1"
                     /translation="MEPVTHFLTGACLGRAGLNRKTGLATLTLILAAEAPDLDVLGRF
                     RGPAFGFAHHRGFTHS"
     gene            complement(315..1265)
                     /locus_tag="VEJ00_06240"
     CDS             complement(315..1265)
                     /locus_tag="VEJ00_06240"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXX00793.1"
                     /translation="MRKAALLYNPDSGGSGRRRLKELESALQVLRAGGVEADLVPTES
                     RHHAGDEARQAAGAGCDTIFACGGDGTIHNIAQVLAKTPVALGILPMGTANALAHDLG
                     LPAKIGAAAGAALHGRLRRIALGCVKYTSFQGTERTRYFVVAAGMGVDAHLFYKLHTG
                     AKQRMGMAAYYAKAWNLWFTYPMTRFVAEYVGDDVGATRVDVTELLSVRIRNFGGVIQ
                     ELAPGASLERDDMRLVLCRTASRMAYLAYVTRGLLRRRWKIPGIELVYSARVSCNYAA
                     SSLAPLPQKVYVEADGELLGTLPAEITVVPDALTLLVPSC"
     gene            complement(1330..1848)
                     /locus_tag="VEJ00_06245"
     CDS             complement(1330..1848)
                     /locus_tag="VEJ00_06245"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX00794.1"
                     /translation="MPKGSGAPKTIAVEKPNVYPLPVALLNAFNTNNRINQYLIDNLP
                     ADAWNAKPREGKGRTIAAIAAHMHNVRVMWLKAVKAEEIPDQLDRSTVTPSQASRALE
                     NSRHALSVVISRALSGDGRIKGFRPDVAGFLGYLIAHDAHHRGQITMLARQLGHPLPQ
                     KAMFGMWEWGRR"
     gene            1981..3627
                     /locus_tag="VEJ00_06250"
     CDS             1981..3627
                     /locus_tag="VEJ00_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00795.1"
                     /translation="MKTLAHVLCAALLGSFLTAQDHASHLHTQPVTLVKGLGDLHHPV
                     STHNPQAQQFFDQGLRFIYAFNHDEAARSFQHAAELDPKLAMAYWGVAEAVGPNYNDP
                     ADPERYKRAHEAIQKAVDLSGNALPSEQAYIQAMARRFPADPGSDLRKAAEDYRDAMR
                     EVAAKFPDDLDAATLFAEAGMNLHPWGLWHQDGTPQAGTEEIVSTLESVIKRDPNHLG
                     AIHYYIHAVEASNNPERALAGANKLAALAPAAGHIVHMPAHVYIRTGDYEAAVKTNEQ
                     AAEVDRAYIKASGAQGIYPMMYYSHNLHFIAMCGAMNGRYEEARKNADLLAANVGPHV
                     KEMPPLEGFMTIPVAVEIRFHHWNEILKMPAPDPAMKTATVFWHFGRGLALAGTGKIA
                     DADAEYKIVADAEAATPPDVIFQMPINNKAKDIMKIAEDVLGAKIAIARKDQPAAISQ
                     LRDAVAIQDKLNYGEPPDWFYPVRENLGGALLMSGDAAGAEKVFREDLDRNPRNPRSL
                     WGLRQALLEQKRDYDAGFVQKQFEAAWKGSPQALKLDDLV"
     gene            complement(3706..4236)
                     /locus_tag="VEJ00_06255"
     CDS             complement(3706..4236)
                     /locus_tag="VEJ00_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX00796.1"
                     /translation="MREILETSRLILREFSPLDADALALVLSDPETMRYYPAALDRAG
                     VEQWIARNMLRYAEHGHGLWAMVLKSTGEMIGDCGLTVQNVDGIDEVEIGYHVLRNLW
                     GQGLAAEAAQACRDYGFARLPVERVISLIRPENLPSRRVAEKNRMMVWKEVMWRGLPH
                     LVYAVGRSTVTQAETL"
     gene            4281..>4842
                     /locus_tag="VEJ00_06260"
     CDS             4281..>4842
                     /locus_tag="VEJ00_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00797.1"
                     /translation="MPASTKDFEKKLDSLREKIRHHEYLYYVLDQPEISDLDFDELMQ
                     QLKDLEAEHPNLITADSPTQRVGGKPREGFIKVPHSSPMLSLDNTYNEEELRDWERRV
                     HELSGRKDVDYVCELKLDGMSLALTYEDGILVRGVTRGDGSVGEDVTLNVRTVHSIPL
                     SIDKGKLKKAGIPADFEARGELLMPAA"
BASE COUNT         1003 a         1599 c         1357 g          883 t
ORIGIN      
        1 aaggaatgcg tgaagccgcg gtgatgcgcg aatccaaacg cgggcccgcg aaatcgcccc
       61 agaacatcaa gatcgggagc ctccgccgcc agaataagcg tgagcgtcgc cagtccggtc
      121 ttgcggttaa gcccggcgcg acccaggcat gctccggtca gaaagtgggt aacgggttcc
      181 aagcggttcg gagaattcta ttgcgccagg cttgactatc gccagtccgc ggcccctgca
      241 aggattctgc gacgttaatt cgactctgcg acagaaacga agtcgcaaag aaattcgctc
      301 ggcgaaggtc acgtttagca agaaggaacc agcagcgtca gcgcgtccgg aacgacggta
      361 atctccgccg gcagcgtccc cagcagttcg ccatccgctt ctacatagac cttctgcggg
      421 agcggcgcta acgaagaagc cgcgtagttg cacgaaactc gggcgctgta cacgagttca
      481 atgcccggga tcttccaccg gcggcgcagc agtccccggg tcacataggc gaggtacgcc
      541 atccgggagg ccgtgcggca caaaaccagc cgcatgtcgt cgcgctcgag cgacgcgccc
      601 ggggccagtt cctggattac ccctccaaag tttctgatgc gcacgctcag cagctccgtg
      661 acatccactc gcgtggcccc gacatcgtcc cccacgtact ccgccacaaa tcgcgtcatg
      721 gggtacgtga accacaaatt ccaggccttg gcataatagg ccgccatgcc catgcgttgc
      781 ttggccccgg tgtgaagctt gtagaacagg tgggcgtcca cacccattcc cgcagccacc
      841 acaaaatagc gcgtgcgttc ggtcccctga aagcttgtgt atttcacgca gccaagcgcg
      901 atgcggcgga gcctgccatg caaagccgcc cccgccgcag ccccaatttt tgcaggtaga
      961 ccaagatcgt gcgccagcgc gttggccgtt cccattggca gaatgccaag cgccaccgga
     1021 gtcttggcca gcacctgcgc gatgttgtga atcgttccgt cgccgccgca ggcgaaaatc
     1081 gtgtcgcatc ccgcaccggc cgcctgccgg gcctcgtctc cggcgtggtg tcgggactcc
     1141 gtcgggacca gatccgcctc cacaccgccg gcacgcagca cctgcagcgc ggattccaat
     1201 tccttcaggc gccgtcggcc actcccgccg gaatccggat tgtagagcaa cgcggccttg
     1261 cgcatggcgg gatcgaagct ggattttacc tgaggccgcg ggccatcagg gcagggccgc
     1321 ggaggaccct cagcgccttc cccactccca catgccgaac atagccttct ggggcaaggg
     1381 atgccccaac tgccgcgcca gcatcgtgat ctgcccgcga tggtgggcat cgtgggcgat
     1441 cagatagcca agaaacccgg ccacgtcagg ccggaacccc ttgatgcgcc cgtcgccgct
     1501 cagcgcgcgg ctgatcacga cgctgagagc gtgccggctg ttctccagcg ctcgcgaagc
     1561 ctgcgacggg gtgaccgtgc tgcgatcaag ttgatcggga atctcctctg ctttcactgc
     1621 cttcagccac atcacgcgca cgttgtgcat gtgcgccgcg atcgcagcaa tggtgcgccc
     1681 tttgccctcc cgcggcttcg cgttccacgc gtccgccgga aggttgtcga tcaaatactg
     1741 attgatgcga ttgttggtgt taaacgcgtt gagcagcgca accggcaagg gatacacgtt
     1801 gggtttttca actgcgattg tcttgggtgc gcccgatccc tttggcatgg ccttcgcaaa
     1861 gcgcttggca aggcccccaa tttgtcataa cctgaacgca ctcgcaagtg ctgtttttcc
     1921 acagctggtt gttaatgttg gggctcgcat ttcaaatcga aacttagggg gattcaccag
     1981 atgaaaactc tggcgcatgt cctttgtgcc gcgctgcttg gctcgtttct caccgcccag
     2041 gatcacgcct ctcatctaca cacccaacct gtcaccctgg taaagggcct cggcgatctt
     2101 caccaccctg tctccacgca caatccgcaa gcccagcagt tctttgatca gggtctccgc
     2161 ttcatttatg ccttcaatca cgacgaggct gcgcgctcat tccagcatgc cgccgaactg
     2221 gaccccaaac tggcaatggc ctactggggc gtcgccgaag ccgttgggcc gaactacaac
     2281 gaccccgccg acccggaacg ctacaagcgt gctcacgaag ccatccaaaa ggccgtcgac
     2341 ctgtcgggca acgctttacc cagcgagcag gcctacattc aggctatggc caggcgtttt
     2401 cccgccgatc cgggttctga cttgaggaag gccgccgaag actatcgcga cgccatgcgc
     2461 gaagttgctg ccaaattccc cgatgatctc gacgccgcta ccctgttcgc cgaagccggc
     2521 atgaacctcc atccctgggg actctggcat caggacggca cgccccaagc tggcacggaa
     2581 gaaatcgtct cgacgcttga gtccgtgatc aagcgcgatc ctaaccacct gggcgcgatt
     2641 cactattaca ttcacgcggt cgaggcctca aacaatccgg aacgcgcctt ggccggcgca
     2701 aacaaactcg ctgcccttgc tcctgcagcg gggcacatcg tccacatgcc cgcgcacgtc
     2761 tacatccgca ctggagacta cgaagccgcc gtgaagacca acgagcaggc cgccgaagta
     2821 gatcgcgctt acatcaaggc gagtggcgcc cagggcatct atcccatgat gtactacagc
     2881 cacaatctgc acttcatcgc catgtgcggc gctatgaatg gacgttatga ggaggcccgc
     2941 aagaacgccg accttctggc cgccaacgta ggcccgcacg tgaaagaaat gccgccgctc
     3001 gagggcttca tgaccatccc cgtggcggtc gagatccgct ttcatcactg gaacgaaatt
     3061 ctgaaaatgc ccgcgcccga ccccgccatg aaaaccgcaa cagtcttctg gcactttggc
     3121 cgcggcctgg ctttggcggg aaccggcaag atcgccgatg ccgatgcgga atacaaaatc
     3181 gtcgccgatg ccgaggctgc cactccgccc gacgttatct ttcagatgcc catcaacaac
     3241 aaagccaaag acatcatgaa gattgccgaa gatgtcctgg gcgcaaagat agctatcgct
     3301 cggaaggacc agccggccgc gatctctcag ctccgcgacg ccgtggccat tcaggacaag
     3361 ctgaactacg gagagcctcc cgactggttt taccccgtgc gcgagaacct gggcggcgct
     3421 ttgctgatgt ccggagacgc cgccggcgcg gaaaaagtct tccgcgaaga tctcgaccgc
     3481 aatccgcgca atccccgctc cctctggggc cttcgccagg ccctgctcga gcagaagcgc
     3541 gactacgatg ccggattcgt gcagaagcag tttgaagcgg cctggaaggg cagcccgcaa
     3601 gcgttaaagc tggatgactt ggtctagagg agatcgtgtt ggggcggacg cagcttgccc
     3661 tgagcgaagt cgaagggtcc gccccgccga agcgaagcgc agtcgtcaaa gcgtttcagc
     3721 ctgagttacc gtgctcctgc caacggcata taccaggtgg ggcagcccgc gccacatcac
     3781 ttccttccac accatcatgc gattcttttc cgcgacgcgg cgcgacggta ggttctccgg
     3841 acgaatcagc gaaatcaccc gctccaccgg caggcgcgca aatccatagt cgcgacatgc
     3901 ctgcgccgcc tccgctgcca acccctgccc ccaaagattg cggagcacgt ggtacccgat
     3961 ttctacttcg tcgatgccgt cgacgttttg cactgtaagc ccgcaatcgc caatcatctc
     4021 gccggtcgac ttgagcacca tggcccagag tccgtggccg tgctcggcgt agcggagcat
     4081 gttgcgcgcg atccactgtt caactcccgc gcggtccaga gcggctgggt agtagcgcat
     4141 tgtttcagga tcggacagaa cgagtgccaa ggcgtccgcg tctagcggcg agaattcgcg
     4201 caggatcaag cgcgatgtct cgagaatctc cctcatcctc ctgattctat gtgaaggcgc
     4261 tggcagatat aatctgcccg atgcccgcct ccaccaagga cttcgaaaag aagctcgact
     4321 ctctgcgcga gaaaatccgc caccacgaat atctctacta cgtcctcgac cagcccgaga
     4381 tcagcgacct cgacttcgac gagctaatgc agcaactaaa ggacctggaa gccgagcacc
     4441 ctaatctgat tacggccgac tctcccactc agcgcgtggg cggcaagcct cgcgaaggtt
     4501 tcatcaaagt cccgcactcc tcgcccatgc tctcgctcga caacacgtac aacgaagagg
     4561 aactccgcga ctgggagcgc cgtgtgcacg aactcagcgg ccgcaaagat gtcgactacg
     4621 tttgcgaact gaagctcgac ggcatgtcgc tggccctgac ctacgaagat ggcatcctgg
     4681 tgcgcggcgt aacccgagga gacggatcgg tcggcgagga tgtcacgctc aatgtccgca
     4741 ccgtccactc catcccgctt tccatcgaca agggcaagct caaaaaagca ggcattccag
     4801 cagattttga agcccgcggc gaattgctga tgccggccgc ag
//
LOCUS       DASPUL010000249         5224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1409712, whole genome shotgun sequence.
ACCESSION   DASPUL010000249 DASPUL010000000
VERSION     DASPUL010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1409712"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3054)
                     /locus_tag="VEJ00_06265"
     CDS             complement(<1..3054)
                     /locus_tag="VEJ00_06265"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXX00798.1"
                     /translation="MSRPRIAFVCLFAALLCNAFSAQAQSTFGSIVGVVKDPNGLVIP
                     GAKVTLASLDNQTTREAVSDPDGAFQFMNVKAGRYEVTAVAEGFAVFKLQSMELEARQ
                     TLRADITLKVKSATETIEVGDLAPMINTESATLADTKDFLQVSQLPVNYRGATTSSLA
                     VLDTVPGTQQDANGNVSVGGGLPSQVQYSVDGASTVNIRQNGALANMNPSSELISEFK
                     VSQFNNNAEFAQLGDITIATKSGTNRFHGSAFEYMQNSVLDATTYGFTSKPHKAFNTF
                     GGSVGGPLKIPGLMKGEAHTFFFGAYEGNRRRLVTPLFLNVPTADERAGNFNGVASIV
                     NPFTGVAFPNDTIQTCGQPGCINPVAQSLLNNYDMALPNSNIPGSNYLQQASTPSNTN
                     GYDLRIDHTLTAKQSLYARWSSKSISTTVPNPLLPSDMDRESDHNLIFSHNYAITNSL
                     VNETRFGLSLFRIGVNFPIEGASAVQQLGLVGLDLSDHPTQGAFPTFNFNDGTGLSPI
                     GRDKAGVTKSQTIQFTDNLGWVRGKHSMKFGVDVRRVAYADIESFGGSDDFGAFTFLS
                     AGGFTGNAFADFLLGLPSKTYVAQSGPDVRAHTLQTGVYGQDEWRVNPRLTLSFGLRW
                     QALPPFVSPLTNLTAFDKTNGGVIVPDHNVPVQGFLESINACAGPYNPYGVANPALPC
                     GPVESASSRGLGPGVREFYKRNFQPRFGFAYRPFGDEKTVVRGGFGLFTMTNLGQLSF
                     NTTNISVAVVRTTANAFQAGQPAYQFPNVRTPDSPLTIAGTGDFYQNTPINYRDPQSA
                     QWNLTVERQLLPDTSLRVSYVGTNSYRMSQTVDLNQVQPSTISPNPNSRPYPNWGRIL
                     SSENEGSVNYEGLQSELNHRSRGGLTLQASHVWAKSLGNVGGDAPTAFSPEVIYGTPV
                     ANRFDLAANRGNIAAVRRHRVLVSAIYDLPFGSGRRFFQGMNRVPQAFFGGWSLSTIG
                     LWQKGPFLTPITSPGYDPGNLNLVYRGAFQRPDCIGDGNLANPTPG"
     gene            3394..4557
                     /locus_tag="VEJ00_06270"
     CDS             3394..4557
                     /locus_tag="VEJ00_06270"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HXX00799.1"
                     /translation="MTSRASDPSELLFWCAVGFVAGIGLFFYGFRLLQRRRLILDTPF
                     SKIRSAPIGMVEVSGLAVGPYTMIAPITARACYYYRTLVSEWKQQGKNKQWVRIAGEC
                     MHVPFFLDDNTGRVLVDPRGADLDLYCDFREEFNGSFFSSRDDAPGNVNSFLARHGIV
                     TSNRIKVEEYCIKPKNSLFILGTLTENTGLTLSPQPVLDDESLGNGVSVSLGSFSFTL
                     NTSGNDRDGGAFVRQLAAPLSPGKPQVVRLYTGSGPLKTTDMTQQQKVAAALLKAGIS
                     NPAAWEAAGVNTADATSPDVQVMADAPLIRSGENGNGLSNDSAPATSTEGFDPHPSVV
                     LKRGENNKTFLISWRSQREVATSLGWKCTLMIWGGPVLALISLYFFLGIRNLF"
     gene            4752..>5224
                     /locus_tag="VEJ00_06275"
     CDS             4752..>5224
                     /locus_tag="VEJ00_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00800.1"
                     /translation="MNDAVRIGILGDFNTEFRSHHATNEALQHAAHKLKMTVESEWVP
                     TSSLTPADTEKKLDSFDGLWAAPGSPYLSFDGMLRGIEFARRRDWPFLGTCGGFQYAF
                     IEFARNVLNIKDADSAENNSGSKNIVIYPVACAVPNRKGDAPKLSGAVPKIRLRPG"
BASE COUNT         1059 a         1477 c         1625 g         1063 t
ORIGIN      
        1 gccgggcgtc ggattggcga ggttgccgtc gccgatgcag tcggggcgct ggaaggctcc
       61 acggtagacg agattcagat ttcccggatc atagccaggg ctggtgatgg gtgtaaggaa
      121 cggacccttc tgccacaagc caatggtgct cagcgaccat cctccgaaaa acgcctgtgg
      181 cactcgattc attccctgaa agaagcgccg gccgctgccg aatgggagat cataaatagc
      241 cgaaaccagc acacgatgtc ggcgcaccgc ggcaatgttg ccacgattgg cagcaagatc
      301 aaagcggttc gctacgggag tcccgtaaat gacttccgga gagaaggctg tcggtgcatc
      361 gccgccgacg ttgcccaggc tcttggccca cacatggctg gcctgcaggg taagtccccc
      421 tcgggagcga tggttcagtt ccgactggag accctcgtag ttcacggacc cctcgttctc
      481 cgaagacaga atgcgtcccc agttcgggta gggccgagaa ttgggattgg gcgagatggt
      541 gctgggctgc acctgattca agtcgacggt ctggctcatg cggtaagagt tcgtgccgac
      601 atagctgaca cgcaggctcg tatccggaag cagttgccgc tcgacggtga gattccactg
      661 cgctgattgc ggatcgcgat agttgatcgg cgtgttctgg tagaagtctc ccgtccccgc
      721 gatggtcaag gggctgtccg gggtgcgaac gttggggaac tggtacgcgg gctgcccggc
      781 ttgaaaggcg tttgcggttg tgcgcacgac cgcaacgctg atattcgtcg tgttgaacga
      841 gagctggccc aggttggtca tagtgaagag gccgaagccg ccccggacca ccgtcttctc
      901 atcgccgaat ggacgatagg cgaatccgaa acgcggttga aaattccgct tgtagaactc
      961 gcgtacaccc ggtcctagtc cccgcgaact ggccgattca accgggccgc agggaagcgc
     1021 ggggttggcc acgccatacg gattgtaggg gccggcgcag gcattgatcg attccagaaa
     1081 tccctgtacc ggaacattgt gatcgggaac gatcacgccg ccattggttt tgtcgaaggc
     1141 ggtgagattg gtcaacgggc tgacgaacgg aggcaaggcc tgccagcgca ggccgaagct
     1201 caacgtgagg cgcggattga ctcgccattc atcttggcca tagacgccgg tctggagagt
     1261 gtgggcccgc acatcggggc cggattgcgc gacgtaggtc ttggagggca gcccgagcaa
     1321 gaagtcggca aaggcattgc ccgtaaagcc gcctgcagac aggaaggtga aagcaccgaa
     1381 atcgtcggag ccgccgaagc tctcgatgtc ggcataagcc acacggcgca catcgacacc
     1441 gaacttcatg ctgtgcttgc cccgaaccca ccccaggttg tcggtgaact ggatcgtctg
     1501 cgacttggtg acgccggcct tgtcacggcc gataggactc aggcctgtgc cgtcgttgaa
     1561 gttgaatgtg ggaaaagctc cctgcgtcgg atgatcgcta agatcgaggc ccaccaaacc
     1621 aagttgctgg accgcactcg ccccttcgat cgggaagttc acgccaattc ggaacagcga
     1681 caaaccgaaa cgggtttcgt tcaccagcga gttggtgatg gcgtagttgt gggaaaagat
     1741 caggttgtgg tcactctcgc ggtccatgtc gctgggcaag agcgggttag gcactgtggt
     1801 ggagatactc ttcgaactcc accgcgcgta cagggactgc ttcgcggtga gagtgtgatc
     1861 gatgcggaga tcgtaaccgt tggtgttgct cggcgtggag gcctgctgca ggtagttcga
     1921 tcctggaatg ttggagttgg gaagcgccat gtcgtagttg ttgagcagcg actgggctac
     1981 gggattgatg cagcccggtt ggccgcaggt ctgaatggtg tcattgggaa acgcgacgcc
     2041 cgtgaacgga ttcacgatcg aggcaacgcc gttaaaattg ccggctctct catccgcggt
     2101 cggcacgttg aggaagagcg gagtcacgag gcggcggcga ttgccttcgt aggcgccgaa
     2161 aaagaacgtg tgggcttcac ctttcatcag gcccggaatt ttcagcggac caccgacact
     2221 tccaccaaag gtgttgaagg ccttgtgagg cttgctagtg aagccgtaag tggtggcgtc
     2281 gaggacgctg ttctgcatgt actcgaacgc gctgccgtga aagcggttcg ttccgctctt
     2341 ggtggcgata gtgatatccc ctaactgcgc gaactccgca ttgttgttga actgcgaaac
     2401 cttgaactcg ctgatcaact ccgacgaggg attcatgttc gccagcgcgc cgttctggcg
     2461 gatgttgacg gtggaagcgc cgtcaacgga atactgcact tgcgagggca atccgccgcc
     2521 cactgagacg ttgccgttgg catcctgttg cgtgccagga acggtgtcga gcaccgccag
     2581 cgagcttgtc gtggccccgc ggtagttgac cggcaactgc gaaacctgca gaaaatcttt
     2641 ggtgtcggcc agagtcgcgc tctccgtgtt gatcatcggc gccagatcgc ccacttcaat
     2701 cgtctcggtc gcggatttca ccttgagcgt aatgtcggcg cgcaaggttt gccgcgcctc
     2761 cagttccatg gattgcagct tgaagacggc aaagccctcg gccactgctg tgacttcgta
     2821 gcgaccggct ttgacgttca tgaactggaa agcaccgtca ggatcagaga ccgcctcgcg
     2881 cgtggtttgg ttgtccaggc tggccagcgt gacctttgcc cccgggatca ccaggccgtt
     2941 cggatcttta acgactccaa cgatcgagcc aaacgtcgac tgcgcctgag cagaaaaagc
     3001 gttgcacaac aaagcggcaa acagacacac gaatgcgatc cgaggtcgag acactgcacc
     3061 cctcctgaag tcgatgttga attgggagac cctgcttaca tcgagctttg agttgcacat
     3121 tagctggact tacataaaga agacataaag aattcgacaa aagtttcgaa tggccgagat
     3181 tcgaattcgc gatgtcgtca aagttttgaa tgggagagac ttgaatcggt gatgccggct
     3241 cgaagcgcga cgcaacacgc cgcgcacgaa tttcattacg aaggtaaggg gcgagcagcg
     3301 tttcctgatg cgcctgcgct gggcagttcg ctagcctgaa ctcggggcag agtgtatcga
     3361 gtcccgactc ttcctatcgt gattcctgct gccgtgacat cgcgcgcgtc cgatcccagc
     3421 gaactgctgt tctggtgtgc ggttggcttt gtcgctggaa ttggcctgtt cttttacgga
     3481 tttcgcctgc tgcagcgccg tcgtctcatc ctcgacactc cgttctccaa gattcgcagc
     3541 gcgccgatcg gcatggtcga agtcagcggc ctcgcagtcg gtccgtacac gatgatcgcg
     3601 cccatcacgg cgcgagcctg ctactactac cgcacgctgg tttcggaatg gaagcaacag
     3661 ggcaagaaca agcagtgggt caggatcgcc ggcgagtgca tgcatgtgcc gttcttcctc
     3721 gacgacaaca ccggtcgggt tctggtggac ccgcggggcg ccgacctgga tctctactgc
     3781 gacttccggg aggagttcaa cggatcattt ttctcgagcc gggacgatgc gccgggaaat
     3841 gtcaacagct tcctcgcgcg tcacggcatc gtgaccagca accgaatcaa ggtagaggaa
     3901 tactgcatca agcccaagaa ctcgctgttt attctgggca ctctgacgga gaacacgggg
     3961 cttacccttt cgccgcaacc ggtgttggac gacgagagtc ttggcaacgg cgtttcggtg
     4021 tctctcgggt cgttctcttt caccctgaac acaagcggca acgatcggga tggaggtgcg
     4081 ttcgtccggc agctggctgc cccgctcagc ccgggtaaac cgcaggtggt ccggctttac
     4141 acgggctcgg gcccgctgaa gacgacggac atgacccagc aacagaaggt tgccgccgcc
     4201 ctgctgaagg caggtatttc gaatccggca gcctgggagg ccgcgggagt gaatacggcc
     4261 gatgcgacct caccggacgt gcaggtcatg gccgacgctc cgctaatccg ctccggcgaa
     4321 aatgggaacg ggttgagtaa tgattcggcg ccggccacgt cgacagaggg gttcgatccg
     4381 catccatcgg tagtgctgaa gagaggcgaa aacaacaaga cgttcctgat ttcttggcgc
     4441 agccagcggg aggtcgcaac ctccctgggc tggaagtgca ctttgatgat ctggggaggc
     4501 ccagtcctgg cgctgatcag cctttatttc ttcctcggca tccggaatct gttctaggga
     4561 agcgtctgcc gctggacttt cgccagctct ggcggcgcgg ctggaagccg cgccctttca
     4621 aaaccgactg atgaaatagc tcagagcttc ttgatttgcg ggtgcacgtt ctgcgtcggc
     4681 gcaccacgct tctaaccctg ccctgctcca ctcctgcggg gaggccgcgc gccctctagt
     4741 acaatcaacg cgtgaacgac gctgtccgca tcggaatcct gggtgatttc aacacggagt
     4801 tccggtccca tcacgctacg aacgaggccc tccagcacgc ggcgcacaag ctgaaaatga
     4861 cggtcgaatc cgagtgggtg cccacttcct cgctgacgcc cgccgacact gaaaagaaac
     4921 tcgactcttt tgacggcctg tgggcggcgc ctggcagtcc ctacctgagt ttcgatggga
     4981 tgctgagggg aattgagttc gcccgacggc gggactggcc gtttctcggc acgtgcggcg
     5041 gcttccagta cgcctttatc gagtttgcga ggaacgtgct caacataaaa gacgctgaca
     5101 gcgccgagaa caattccgga tcaaagaata tcgtgatcta tcccgtggcc tgtgcggttc
     5161 ccaaccgcaa aggggatgct cccaaacttt ccggagccgt gcccaagatt cggctgcgcc
     5221 cggg
//
LOCUS       DASPUL010000250         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_727253, whole genome shotgun sequence.
ACCESSION   DASPUL010000250 DASPUL010000000
VERSION     DASPUL010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727253"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VEJ00_06280"
     CDS             complement(<1..578)
                     /locus_tag="VEJ00_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00801.1"
                     /translation="MVDLSTTYLGLKLHTPLVVSASPLSRDVEAICRLEEAGASAVVL
                     YSLFEEQLRQEEVDLEYHLSAGTESFAESLTYFPQASEFQTGPEGYLNHIRKAKSSVR
                     IPIIASLNGSTMGGWTKFASEIERAGADAIECNVYYIPTDPKLRAEDVEKTYVDILRA
                     VKSAVTIPVAVKLSPFFSNMANMAQRLDAAGA"
     gene            complement(606..>2570)
                     /locus_tag="VEJ00_06285"
     CDS             complement(606..>2570)
                     /locus_tag="VEJ00_06285"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXX00802.1"
                     /translation="GMGSRMNVIMQVCFFAISKVLPGEEAIEAIRQSIRDTYGRKGED
                     VVKKNMLAVDETLAHLFEVKVPASVTSTTEMPEVFPGAPKFERDVLGTIYSGRGDELP
                     VSAFSCDGTFPTATARWEKRNLALEIPAWDSKICIQCGKCAMVCPHAVIRIKVYDSKE
                     LANAPATFKSCEARDKEWAGMKYTIQVAPEDCTGCGVCVDVCPVKNKAETRLKAINMV
                     PQPPLRAPEKENWEFFLNIPELDRRKIKVGNIRQQQVQEPLFEFSGACSGCGETPYIK
                     LVTQLFGDRTIIANATGCSSIYGGNLPTTPYTKNADGRGPAWSNSLFEDNAEFGLGFR
                     ISIDKQAEFARELLHGLAEKVGDDLAQQILVAKQKDEADIFEQRQRVDVLKERLQKVN
                     SPEAKQLLAVADMLVKKSVWIVGGDGWAYDIGYGGLDHVLASGRNVNLLVLDTEVYSN
                     TGGQASKSTPRAAVAKFAAGGKPGPKKDLGLMAMTYGNVYVASVAMGAKDEHTLKAFL
                     EAEAYDGPSIIIAYSSCIAHGIDMTTSMTDQKVAVESGQWLLYRYNPERAEAGENPLV
                     LDSRAPTRKVQEYLLQQTRFKMLTKSKPEDAERLWKLAQQDAEHRYRMYEYMAARTSG
                     PAAAPKEPATKPAAGAEKPASEKPTTTAAR"
BASE COUNT          435 a          795 c          782 g          558 t
ORIGIN      
        1 tcggcgcccg cggcgtccag gcgctgcgcc atgttggcca tgttgctgaa gaacgggctc
       61 agcttgaccg cgaccggaat tgtcactgcc gacttcaccg cacgcagaat gtcgacatag
      121 gttttttcga catcttccgc cctcagcttc ggatcggtgg ggatgtaata cacgttgcac
      181 tcgatggcat cggcaccggc gcgttcgatc tccgaggcga actttgtcca tccgcccatg
      241 gtcgagccgt tcaggctggc gatgattgga atgcgaaccg aagacttggc cttgcggatg
      301 tgattcaaat atccttcagg tccggtctgg aattcgctgg cttgcgggaa gtaggtcaac
      361 gactcggcaa aactttccgt gcccgcgctc aggtggtact ccaggtcgac ctcctcctgc
      421 cgcagctgct cttcaaacag cgaatacagg acaactgccg aggcgccggc ttcctccagc
      481 cggcagatag cctccacgtc gcgcgacagc ggtgatgccg agaccacaag aggcgtgtgc
      541 agcttcaatc caagatacgt tgtgctgagg tcgaccataa ttcctcctta cagcggctca
      601 cccggctagc gcgctgcggt cgtcgtgggt ttttccgagg ccggcttctc tgcgccggca
      661 gcgggcttcg tggcgggttc cttgggtgcg gctgccggtc ccgacgtgcg cgcggccata
      721 tattcgtaca tgcgatagcg atgctccgca tcctgctgcg ccagcttcca cagacgctcg
      781 gcgtcttccg gcttgctctt ggtcagcatc ttgaagcgcg tctgctgcag caggtactcc
      841 tgcaccttgc gagtcggagc acgggaatcg agcaccagcg gattctcgcc tgcctcggcc
      901 cgctccgggt tgtagcggta gagcagccac tggccggact cgaccgcaac tttctggtcg
      961 gtcatcgaag ttgtcatgtc gatgccgtgt gcgatgcacg acgagtaggc aatgatgatg
     1021 ctgggcccgt cgtacgcctc ggcctcgagg aacgccttga gcgtatgctc gtccttggcg
     1081 cccatggcca cgctggccac gtagacgttg ccgtaggtca tggccatcag cccgagatct
     1141 ttcttgggac ccggcttgcc gcccgcagca aacttggcga ccgccgcgcg cggcgtggac
     1201 ttggaagcct gcccccccgt gttcgagtaa acctcggtgt cgagcacgag caggttgacg
     1261 ttgcgcccgc tggccagcac gtggtcgagg ccgccgtagc cgatgtcata ggcccagccg
     1321 tcgccgccga cgatccacac gctctttttc accagcatgt cagcgacggc gagaagctgc
     1381 ttcgcttctg gcgagttcac tttctgcagc cgctctttga ggacgtccac gcgctggcgc
     1441 tgctcgaata tgtcggcctc gtctttctgc ttggcgacga gaatctgctg cgccaggtcg
     1501 tctcctacct tctcggccag accgtgcagc aattcacggg caaactccgc ctgcttgtcg
     1561 atggagatgc ggaagcccaa accgaattcg gcgttgtctt caaacagtga gttcgaccac
     1621 gccgggccgc ggccgtcggc gttcttggtg taaggagtgg tcggcaggtt gccgccgtaa
     1681 atggacgagc atcctgtggc attcgcgatg atggtgcgat ctccgaacag ctgcgtgacc
     1741 agcttgatgt agggcgtctc gccgcatccg gagcaggcgc cagaaaactc gaacagcggc
     1801 tcctggacct gttgctgccg gatgttcccg accttgatct ttctgcggtc gagttcagga
     1861 atgttgagga agaattccca gttctctttc tctggcgcgc gcagcggcgg ctgcggcacc
     1921 atgttgatcg ctttcaggcg cgtctcggcc ttgttcttga ccgggcagac atcgacgcag
     1981 acgccgcatc cggtgcagtc ttcgggcgca acctggatcg tgtacttcat tcccgcccac
     2041 tctttgtcgc gcgcctcgca ggatttgaag gttgccgggg cattggcgag ttctttgctg
     2101 tcatacacct tgatacggat caccgcgtgc gggcacacca tggcgcactt cccgcactgg
     2161 atgcagatct tcgaatccca ggcgggaatt tcgagggcga gattgcgctt ctcccagcgt
     2221 gcggtcgcag tagggaacgt gccgtcgcaa gagaaagcgc tgacgggaag ttcgtcgccg
     2281 cggccagagt aaatcgtgcc cagcacatcg cgctcgaact tgggagcgcc ggggaaaacc
     2341 tccggcattt cggttgtgct ggtcacgctg gcgggaacct tcacctcgaa taggtgtgcg
     2401 agcgtctcat cgaccgcgag catattcttc ttgacgacgt cttcgccttt gcggccgtac
     2461 gtgtcgcgga tcgactggcg gatagcctcg atggcttctt ctccaggcag aactttcgaa
     2521 atcgcgaaga agcacacctg catgatgacg ttcatgcgcg agcccatgcc
//
LOCUS       DASPUL010000251         3646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_184602, whole genome shotgun sequence.
ACCESSION   DASPUL010000251 DASPUL010000000
VERSION     DASPUL010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184602"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VEJ00_06290"
     CDS             complement(<1..1096)
                     /locus_tag="VEJ00_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXX00803.1"
                     /translation="MTFPATISELAPRLRRREISPVEITRACLQRIEELNPALNAFIT
                     VMMDSALSDAQKAEAEILRGEWRGPLHGIPIALKDLIDTAGVRTTAASAVHKDRIPSE
                     DADVVRRLRIAGAVIVGKNNLHELAYGGSSLVSYFGEVRNPRDLGKIAGGSSGGSAAA
                     VVAGLAYAAIGTDTAGSIREPAALCGCVGLKPTYGRVSSRGVIPLSASLDHVGPLAAS
                     VADAAIVLQAIAGYDAADITSVDVPVADYVGALREDAKRLRVGVVRDYFFDDLDAEVA
                     AAVDEALRIIEPLVPEMKDMQLEVPTDRKLQSAESFAYHAENVAKSPELYQAETLRRI
                     RTGEKVTAAEYIQRRRELDEARRGIQAAFAE"
     gene            1256..2701
                     /locus_tag="VEJ00_06295"
     CDS             1256..2701
                     /locus_tag="VEJ00_06295"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXX00804.1"
                     /translation="MAEMTIAPVATHGFFIDGHWREDGEAIEIRAPYDGSLIARVIQG
                     RREHAEAAIAAAVKAFGTTRRLPAFERQRVLRRIAASLVERKEEFARTLAQEAGKPIK
                     GARTEVERAIFTFNVAAEESTRIYGEYLPLDWQEFTAGRWGIVRRFPLGPIVGITPFN
                     FPINLVAHKVAPAIAAGCPMVLKPAPQTPLCSLLLAECVQQAGWPDGGLNVLPLSNED
                     AGILVADDRIKLISFTGSVPVGWDIKRRAGKKKVILELGGNAAVIVHSDADLAYAADR
                     CVTGGFAYAGQTCISVQRVLVEHSVYGKFSDLLVEGVRKLKVGDPLDDSTDVGPLIRE
                     SDAARTASWIEEAVRAGARLLCGGGRDGLMVEPTVLTGTKPDMKVNCQEIFGPVVTVE
                     PYRDFDQAIRQVNNSAYGMQAGVFTRDAKLLFQAYEELEVGGVIAGDVPSFRIDHMPY
                     GGVKDSGLGREGLRYAIEEMTEPKLLVMNLR"
     gene            2958..>3646
                     /locus_tag="VEJ00_06300"
     CDS             2958..>3646
                     /locus_tag="VEJ00_06300"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXX00805.1"
                     /translation="MPQIFQRSTNTLSRATIFGAVFVVAALTWIGYEIQGSPYVTYAG
                     VRKPQPVPFSHQHHVTGLGIDCRYCHTSVETSSFAGIPPTKTCMNCHSQIWTNAKLLE
                     PVRASYKSGESLLWTRVNQLPDFVYFNHSIHVAKGVGCNTCHGPVDQMPLMYQQESLQ
                     MEWCLGCHHAPENHLRPRDQVFNMRYQQPTSVNPVVVDGQSYTDQIELGNALVKKYNV
                     RSVKDITTCST"
BASE COUNT          723 a         1192 c         1022 g          709 t
ORIGIN      
        1 cttcggcgaa ggcggcctga atgccgcgcc gtgcttcgtc cagctcgcgc cgccgctgga
       61 tatactccgc cgccgtgacc ttttccccgg tgcgaatgcg ccgcagcgtt tctgcctgat
      121 agagttcagg actcttcgcg acattctccg cgtgataggc gaaagattcg gcggattgca
      181 gcttccgatc tgtgggcacc tccagctgca tatccttcat ttctggcacg agcggctcaa
      241 tgattcgcaa ggcttcatcg accgcggctg ccacctcggc atcgagatcg tcgaagaaat
      301 aatctcgcac cacgcccaca cgcagccgct tcgcatcctc ccgcagcgcg ccgacgtagt
      361 cggcaaccgg aacgtccacc gaagtgatat ccgcagcgtc gtatccggca atcgcctgca
      421 gcacgatcgc cgcatcggca acgctcgcag ccaagggtcc gacgtgatcg agcgacgccg
      481 acagcggaat tactccccgg ctcgacacgc gcccataggt cggcttcagc cccacgcagc
      541 cgcacagcgc cgccggttcg cgaatcgatc ctgctgtatc cgtgccaatc gctgcgtatg
      601 ccaagcctgc gaccacagca gccgccgagc ctcccgacga tcctccagcg atctttccca
      661 gatcccgcgg attacggact tcgccaaagt agctcaccag cgaactgccg ccgtaagcaa
      721 gctcgtgcag gttgttcttg ccaacaatca cggcgcccgc gatccgcaac cttcgcacaa
      781 cgtccgcatc ttcagaagga atgcggtctt tgtggacagc gctggctgcg gtcgtccgca
      841 cgcctgcggt atcgatcaag tctttgagtg cgatcggaat tccatgcagc ggcccacgcc
      901 actccccgcg cagaatctcg gcttcagcct tctgcgcatc ggacagcgca gaatccatca
      961 tcaccgtaat aaacgcattc agagccgggt tcagctcctc aatccgctgc aaacaagccc
     1021 gcgtgatctc gacgggagaa atctccctcc gccgcaggcg cggcgccagc tcagaaattg
     1081 tcgcaggaaa ggtcatgccc ggtgcgagcc cattgtatag ggtggcgaaa cttactctgt
     1141 gaatccctgt attctctgtg gttcattttt tccgaaagtc gacattgcaa tcctctccgt
     1201 caaaagtcct ataatctttc cctgcgggtt ccgctggggg ccaaggaaaa acatcatggc
     1261 agagatgacg atcgctccgg tcgcgacgca cggttttttc atagatggcc actggagaga
     1321 agatggcgag gccatcgaga ttcgcgcccc gtacgacggc agcctgatcg cgcgcgtgat
     1381 ccagggacgc cgagagcacg ccgaagctgc catcgccgcg gcggtcaagg ccttcggcac
     1441 cacgcgccgc ctcccggctt ttgagcgcca gcgcgtgttg cgccgcatcg cggcctcgct
     1501 ggttgaacgc aaggaagaat tcgcccgcac tctcgcccag gaagcgggca aacccatcaa
     1561 gggcgcgcgt accgaagtcg agcgggccat ttttaccttc aatgtcgccg ccgaagagag
     1621 cacgcgcatc tacggcgaat acctgcctct ggactggcag gaattcaccg ctggacgctg
     1681 gggcatcgtg cgccgcttcc cgctcggacc gatcgtcggc atcacgccgt tcaatttccc
     1741 gatcaatctg gtcgctcaca aagtggcgcc cgccatcgcc gccggctgcc ccatggtgct
     1801 gaagcccgcg ccgcaaacgc cgctctgctc gctgcttctg gccgagtgtg tgcagcaggc
     1861 gggctggccc gacggcggac tcaacgtgct ccccctttcc aacgaagacg ctggcattct
     1921 ggtcgccgac gaccgcatca agctgatcag cttcaccggc agcgttccgg tcggctggga
     1981 catcaagcgc cgtgccggaa agaagaaagt catcctcgaa ctcggaggca acgccgccgt
     2041 catcgttcac tcggacgccg acctcgccta cgccgccgac cgctgcgtta ccggaggctt
     2101 cgcctacgcc ggtcagacct gcatttccgt gcagcgcgtt ctggtcgagc actcggtcta
     2161 cggcaagttc agcgatcttc ttgttgaagg agtaagaaaa ctgaaagtcg gcgaccccct
     2221 cgacgactcc accgacgtgg gaccgctcat ccgcgaaagc gatgccgccc gcaccgccag
     2281 ctggatcgaa gaagccgtgc gcgcgggtgc tcgcctgctc tgcggtggcg gccgcgatgg
     2341 tctcatggtc gagcctactg ttctcaccgg caccaagccc gacatgaagg tgaactgcca
     2401 ggagattttc gggccggtcg ttacggtcga accttaccgc gactttgatc aagccatccg
     2461 ccaggtcaac aactccgctt atggcatgca ggccggcgtt ttcacccgcg atgccaagtt
     2521 gctattccag gcctacgagg agttggaagt tggcggcgtc atcgctggcg acgttccttc
     2581 cttccgcatc gaccacatgc cctacggcgg cgtgaaggat tccggcctcg gacgcgaggg
     2641 cctgcgctac gccatcgaag aaatgacgga gccaaaactt ctggtcatga atttgcggta
     2701 gttgcgatcc gctccgcgaa gtccggcggg catcttgccc tcgaaatcgc tgtacccttt
     2761 ttctcatcgc gggcatcgtt catctgtaga atttttcgcg acaatacgcg acaaacgggg
     2821 gcaggactgt ttataatttc cggttccaca acctcgggaa aactagtggg tggttttctt
     2881 tttcgccgaa actgaaccag cttcactttg acagcggacg atccgccggg tctcctcaac
     2941 cagtctcata gaagagaatg ccgcagattt ttcagcgtag tacgaacacg ctctcgcggg
     3001 cgacgatttt cggcgcagtg tttgtcgtag ccgcgctgac ctggatcggt tacgaaatac
     3061 agggatctcc ttacgtcact tatgccggcg tgcgcaaacc gcaaccggtg ccattcagcc
     3121 atcagcacca tgtgaccgga ctcggtatcg actgccgtta ctgccacacc tcggttgaga
     3181 cctcgagttt cgcgggcatt cctccgacga agacgtgcat gaactgccac tcgcagatct
     3241 ggaccaacgc caagctgctc gagccggtcc gcgccagcta caaatcaggg gagtcgctgc
     3301 tgtggacgcg cgtcaaccag ctgcctgact tcgtttattt caatcacagc attcatgtcg
     3361 cgaaaggcgt cggctgcaac acctgccacg ggccggttga ccagatgccg ctgatgtacc
     3421 agcaggaatc gctgcagatg gagtggtgcc taggctgcca tcacgcgccg gagaatcatc
     3481 tgcgcccgcg cgaccaggtg ttcaacatgc gttaccagca gccgaccagc gtgaacccgg
     3541 tggtggtcga cggccaaagc tacaccgacc agatcgaact gggcaatgct ttagtgaaga
     3601 agtacaacgt gcgcagcgtg aaggacatta cgacctgcag cacgtg
//
LOCUS       DASPUL010000252         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_869612, whole genome shotgun sequence.
ACCESSION   DASPUL010000252 DASPUL010000000
VERSION     DASPUL010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869612"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..826
                     /locus_tag="VEJ00_06305"
     CDS             317..826
                     /locus_tag="VEJ00_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00806.1"
                     /translation="MNRAFLAALLSSFLLLSGGCKSKTDNKDAIRDAITKRLASMNGL
                     NVNNMTITVTKATISGDTAQADVEIRAKNGDPNAPAMQLSYDLQKQGEEWVVLKGQST
                     GGMQHPAGGEMPPSGALPPGHPATNGASGQMPANHPDFNAILNSAQPPAQSAPSQPPV
                     QQTATGTKP"
     gene            complement(929..2782)
                     /locus_tag="VEJ00_06310"
     CDS             complement(929..2782)
                     /locus_tag="VEJ00_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXX00807.1"
                     /translation="MADARHTRFAPSQETLIAGFTLVAIFLHVVLKYFLHFPPLRANL
                     PLFAALLIGGIPMTWELVKKLWKREFGSDLLAGVSIITAIVLREYLVAAIVVLMLSGG
                     EALEEFATARASSVLDALARRMPNVAHRKEGGKLIDVKLGDIRIGDELTVLPHEICPV
                     DGTVLEGHGIMDEAYLTGEPYEISKTPGSMVISGAVNGEALLVIRAEKLAEDSRYARI
                     MQVMHQTEQQRPKLRRLGDQLGAWYTPIALGVAIAAALLSGQSTRFLSVVVIATPCPL
                     LLAIPTAIIGAISLAARRAIIIKNPAVLEQIEKCQTLIFDKTGTLTYGRPSLTEVICA
                     EGFERTRVVQLAASLEQYSKHPLASAIIAEAKRAEIFILPVEKISERPGEGLRGTVSG
                     HLVQITGRKKIDPQQFPLPPLESGLECLVFIGGKYGAAIRFHDAPRDESASFIQHLHP
                     KHGVRKVMLVSGDRESEVKYLAESVGITEVHASKSPEEKVAIVKEETAKARTLFVGDG
                     INDAPALMAATVGVAFGSQNDITTEAADAVVMEAALEKIDELMHIARRMRQIALQSAL
                     GGMAASLIGMLAAAFGYLPPIWGAIGQELIDLLAVLNAVRVALPTEDLQDF"
BASE COUNT          638 a          941 c          796 g          590 t
ORIGIN      
        1 tccactgcaa cctgttctgg gccgcccgac tgaccctctt cccgaggctc gccagctggc
       61 gggcctcggg aagctcccac caccgaatgt ttgccatgca gacactctac cgagtgtcac
      121 caatcattct gaacgagcac agtgaacatg ctagccgtgg cacacagata aaacacctgc
      181 cccggctcaa ctcacatacg cccagtgcgg tccctctttt cgaaccagcg tttcgcgttc
      241 ctccgcgggt tgcgctacca tcaaagaagc ggatccaaac accagttcca ctctccgttt
      301 acgaaaggaa atttcgatga atcgggcatt cctcgcggcg ctgctgagct cttttttgct
      361 cctgagcggc ggctgcaaat cgaagacgga caacaaggac gccattcgcg acgccatcac
      421 caagcgcctt gccagcatga acgggttgaa cgtgaacaac atgaccatca cggtgaccaa
      481 agccacgatc agcggcgaca cggcccaggc cgacgtggaa atccgcgcaa aaaatggcga
      541 tccgaacgct cccgcgatgc agcttagcta tgatcttcag aagcaaggcg aagaatgggt
      601 tgttctcaaa gggcagtcga caggtggcat gcagcatccc gcggggggcg agatgcctcc
      661 gtctggcgct ttaccgccgg gccatcccgc aacgaacggc gccagcggcc agatgccggc
      721 aaatcaccca gactttaacg ccatcctgaa cagcgcccag cccccggcac agtcggctcc
      781 ctcccagccg ccggtgcagc aaacagccac cggcacgaag ccttgatgca ggcgaaataa
      841 aggaaatcgg taatcagata acggatggcc gctcccgaac actaactgct aactaccaac
      901 tactgactgc cgacttcaga ctggctactc agaaatcctg caaatcctcc gtcggcaagg
      961 ctacgcgcac ggcattcagc accgccagca aatcaatcag ctcctgtccg atggcgcccc
     1021 aaatcggcgg caaataccca aacgctgccg caagcatccc gatcaagctg gcggccattc
     1081 cgcccagtgc gctttgcaag gcgatctgac gcatgcgccg cgcgatgtgc atcagctcat
     1141 cgattttctc gagggctgct tccatcacca ccgcatcggc agcctctgtc gtgatgtcat
     1201 tctgcgaacc aaatgccacg cccacggtag ctgccatcag tgcgggcgcg tcgttgattc
     1261 cgtcccccac aaacagtgtg cgcgcctttg cggtttcctc tttcacaatg gctacctttt
     1321 cttcagggct cttgcttgcg tgcacttccg tgatccccac actctctgcg agatacttta
     1381 cctccgactc gcggtcgccg gagacgagca tcaccttgcg cacaccatgc ttcgggtgca
     1441 ggtgttgaat gaaagacgcg ctctcgtcac gcggtgcgtc gtgaaatcga atcgccgccc
     1501 catacttgcc cccaatgaac accagacatt ccagcccgct ttcgagcggc gggagaggga
     1561 actgttgcgg atcgatcttt ttccttcccg taatctgcac cagatgtcct gagactgtgc
     1621 ctcgcaagcc ctcgccaggt cgttcgctga tcttctccac cggcaggatg aaaatctccg
     1681 cgcgtttcgc ctccgcaatg atcgcgctgg ccaacggatg tttcgaatat tgctccaggc
     1741 tcgccgccag ctgcaccaca cgcgttcgtt cgaagccctc cgcacagatt acttcggtga
     1801 gcgagggtcg gccgtaggta agtgtgccag tcttgtcgaa aatcagcgtc tggcactttt
     1861 cgatctgctc caacaccgcg gggttcttga tgatgatggc gcgccgcgcc gccagtgaaa
     1921 tcgcgccgat aatcgctgta ggaatcgcca gcagcagtgg gcaaggtgtg gcaatcacga
     1981 cgacggaaag gaagcgcgtg gattgcccgc taagcagtgc cgcggcgatc gcgaccccaa
     2041 gtgcaattgg cgtgtaccac gcaccgagtt gatcccccag ccgccgcagc ttgggtcgct
     2101 gctgctccgt ctggtgcatc acctgcataa tgcgcgcgta tcgcgaatct tccgccagct
     2161 tctccgcgcg aattaccagt agggcctcac cattgaccgc gccggaaatc accatcgagc
     2221 ccggcgtctt tgaaatctcg tacggttcgc cggtcaggta ggcctcgtcc ataatgccgt
     2281 ggccttccag gaccgtaccg tccacggggc agatttcgtg cggcaacacg gtgagttcgt
     2341 cgccgatgcg gatatcgccg agttttacgt cgattagctt gccgccttct ttgcggtgcg
     2401 caacgttcgg catgcgccgc gccagcgcat cgagcacaga tgacgcccgt gccgtcgcaa
     2461 attcctccag tgcctccccg ccggacagca tgagcaccac gatggccgcc accagatatt
     2521 cccgcaacac aatggccgta atgatggaaa ctcctgcaag cagatcagag ccgaattctc
     2581 gcttccagag tttcttcacc aactcccagg tcatcgggat gccgccaata aggagggctg
     2641 cgaaaagggg caggttggcg cgaaggggag ggaagtgcag gaagtacttc agaacgacat
     2701 gcaaaaatat tgcaacaagc gtgaatccgg caatcagcgt ttcttgggag ggagcgaatc
     2761 gagtgtgcct cgcatccgcc attacgccca cttatcccat agcttcaaat tcaagtcaaa
     2821 gatgtgtact cgtccagtag attagtgaca cttgcctttc cgtgggtgcg tttccactga
     2881 cgaagaaatt cgagtggggt gaggtaggcg agggcttggt gggggcggac gcagttgtaa
     2941 atttgttccc aacggcgtag ctgct
//
LOCUS       DASPUL010000253         6230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1145321, whole genome shotgun sequence.
ACCESSION   DASPUL010000253 DASPUL010000000
VERSION     DASPUL010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145321"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="VEJ00_06315"
     CDS             <1..572
                     /locus_tag="VEJ00_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXX00808.1"
                     /translation="MGEPFLNYDNFMKAVRLLVEETNVAESRMTVSTAGIVPRIYDFG
                     QEPLRPKLAISLNASNDVLRTELMPLNRKWSLEKLIMAARDFPLRNRERITFEYVLLR
                     DVNDTTGNAREVVELLRGLRAKVNLIALNPGPGISFETPAEERVAAFQQILIKAGIPA
                     FVRRPRGRDIYAACGQLKRTVEIAAAPIP"
     gene            complement(532..2286)
                     /locus_tag="VEJ00_06320"
     CDS             complement(532..2286)
                     /locus_tag="VEJ00_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXX00809.1"
                     /translation="MPLSARQKLWLVVVAAALSVQLVATFMLHQSFRLTALSDTVQCF
                     LLASATAAFIPLILSSKGRMRWFWSLIALGVAFWFIYQLLWTYHEVLLRHDVPDLYAG
                     DIILFLHIVPLMAALALRPHVPQDEYAARLGRLDFALLLVWWFYLYVLTVIPWQYVVP
                     NVEAYNHNLNAVYNAEKCAFLAGLCVCWIGSQGPWRQLYASLFGMSLSYAATSTLANW
                     AIARNTYYSGSFYDIPMAVAMAWLTWIGLSSKAEAPQSRQREVSTVYGVWVARCGMLA
                     VFSLPVFAAWAMWDAAVPTPIRSFRLVLTLAAALSMGVMVLVRQRLLDRELMQLLSHS
                     EASFDSLKHLQVQIVQSEKLASIGQLVGGAAHELNNPITAMLGYSDMLLNTPLTTEQQ
                     RLAGKIGQCVRRAKSLVAGLISFAQQAPAPQTPTDLNTLARTAVKLTQPQWQALNIEV
                     RAEFDPALPKVLGDSNQLLQVCLQLLGNCLHLMSERGSRLLSVTTENCDGRCVLRIAA
                     DPPASFATGDAQLVPDATDGLALSACEGILKEHHGRMRCESRSDGGMILRVELPAATA
                     SGIHRATVSALLQSQPYA"
     gene            2449..4269
                     /locus_tag="VEJ00_06325"
     CDS             2449..4269
                     /locus_tag="VEJ00_06325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXX00810.1"
                     /translation="MHPQAERYRQSWRLRIPPDPGETRRIERWLATARVFLAVATLVA
                     IRMDPTELGHSWAAYGLFVFYLANGILIMMLLRRRQESTPAFRLLVHTTDIIWPALIS
                     IFSEGPRTPFFLFFFFVLAAAAYRWGLWETLGTAAAEVALLWAESYFLLRVPLGPGRT
                     LPWQFLSGLHVSVGTFEPQRLFMLSIYLIVMGLLLGYLAEQQKHLRAEKAVVTGILSQ
                     VRVEAGLTGTIQQIFRETLSMYGASHLVVASQETHSHRVFLGELMNSGNGALSEFRWL
                     ESKPRDAKTYLEDFPGDVCYATTDRDRWSILALDENGLAVPAVNAAPISQLREVQNFN
                     SLITVAFLFGSDWRGRVFLFNPNWRGEKQEELRFLLDLVRQIGPAIYNVYLLHRLRRR
                     AGAAERARFARELHDGAVQSLIAVEMQVDVVRRQAEAGRTITGELGRIQGLLREEVLK
                     LRELMQQMKSIDVDAPRLLGVLHDTVERFQRETGISARFVTDIDKLDMPQRVCREMLR
                     IVQEGLVNVRKHSGARHALVRLGFNHDQWHLTVEDDGKGYPFTGRLSQTEMEEIGKGP
                     MIIKERVRLIAGELTVESNPGQGTRLEVTVPRSGEVPHEL"
     gene            4259..4945
                     /locus_tag="VEJ00_06330"
     CDS             4259..4945
                     /locus_tag="VEJ00_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXX00811.1"
                     /translation="MNFRKQTVRIVIADDHPIFRDGLRRLLEAEADLKVIGEASDGAE
                     AVKLARTLKPDILLLDLAMPKHPGLEALRDLSVAPGANPVRVILLTAAAEKSQIVEAL
                     QLGARGVVLKDSATQLLLKAIHTVMSGEYWVGRESVSNLVQYLRTLMQSSSDEARQKK
                     FGLTPRELEIVSAVVAGYSNKEIAEYFKISEDTVKHHLSNIFDKLGVSTRLELALFAV
                     NQALPLKSIA"
     gene            5140..5304
                     /locus_tag="VEJ00_06335"
     CDS             5140..5304
                     /locus_tag="VEJ00_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00812.1"
                     /translation="MARLLQQLWNDDSGQDIAEYAVMLAVILVIVVGTIRLIGSNANN
                     VFSQVGSAIQ"
     gene            complement(5439..>6230)
                     /locus_tag="VEJ00_06340"
     CDS             complement(5439..>6230)
                     /locus_tag="VEJ00_06340"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXX00813.1"
                     /translation="VGLLAPPGDIDKILCTVVNNLLVTNNIDLQGDVRCRVLLTSPLE
                     SFTIGHTIVISRGLLDVLPDEASLAMVLSHELGHIVLGHRLDTKLAFNDKMFFPDEDS
                     FQRLDFKRNQADEDAADAKAIELLKNSPYKDKLGNAGLFLKELQEKSPDLPYLIRPHL
                     GNSLAEGKSMRMASLVGTAPALDAKRLDQIAALPLGGRIKVDPWTDQVELSKAKVVAL
                     TSAREKMPFEIAPFFPYLTRLSTGSTEKVALTTTSPSTPAAPEQK"
BASE COUNT         1334 a         1763 c         1901 g         1232 t
ORIGIN      
        1 gcatgggcga accgttcctg aattatgaca acttcatgaa agcggtgcgc ttactggtcg
       61 aagagaccaa tgtggcggag tcgcgaatga cggtttcaac cgccggcatc gtgccgcgaa
      121 tttatgactt cggtcaggaa ccccttcggc ccaagctcgc catctcgctg aacgcgtcca
      181 acgatgtgct gcgtacagag ttaatgccgc tcaatcgcaa gtggagcctg gagaaactga
      241 tcatggctgc ccgcgatttt cccctgcgta atcgcgaacg catcacgttc gagtatgtac
      301 tcctccgcga cgtcaatgac acgaccggga atgcgcggga ggtggtcgag ttgctgcgcg
      361 ggctgcgggc taaagtgaat ctgatcgcgc tgaacccagg gccgggaatc agcttcgaaa
      421 caccagccga ggagcgcgta gccgcgtttc agcagatctt gatcaaggcg gggattccgg
      481 cgttcgtgcg acggccgcga ggccgcgaca tctacgctgc ctgtgggcag ctcaagcgta
      541 cggttgagat tgcagcagcg ccgataccgt agctctgtga atgcccgacg ccgtagcggc
      601 cggcagttcg acgcggagaa tcatgccgcc gtcgctgcga gactcgcagc gcatccgacc
      661 gtgatgctcc ttcaagattc cttcgcaggc actgagcgcg aggccatctg tagcgtcggg
      721 aactaattgt gcatcgcctg tggcaaaaga ggccggcgga tcggctgcaa tccgcaacac
      781 gcaacgaccg tcacaattct cggtggtaac cgacagaagc ctgctgccgc gctcgctcat
      841 caggtgcagg caatttccca ggagttgcag gcagacctga agcagttgat tagaatcgcc
      901 cagaactttc ggcagtgcgg gatcaaattc cgctcggact tcgatattaa gggcctgcca
      961 ttggggttgc gtcagcttga ctgcagtgcg ggctaaagtg ttgaggtcgg taggcgtttg
     1021 cggggctggc gcctgctggg cgaagctgat caggccggcc acgagcgact tggccctgcg
     1081 cacacattgt ccgatctttc ccgccaggcg ctgctgttcg gtggtaagag gcgtattcag
     1141 cagcatatcg gaatagccga gcatggcggt aatcggattg ttcagttcat gcgccgctcc
     1201 gcccaccaac tggccgatcg aagccagttt ctccgattgc acaatttgca cctgcaggtg
     1261 cttgagacta tcgaatgacg cttcagaatg gctcagcaac tgcatcagtt cacggtcgag
     1321 cagacgctga cgcaccagca ccatcacgcc catggaaagc gccgcggcca gcgttaacac
     1381 cagacggaag gaacggatcg gcgtcggaac cgcggcgtcc cacatagccc aggcagcaaa
     1441 cacagggaga gagaacactg caagcatgcc gcagcgcgcc acccagacgc cgtacacggt
     1501 cgagacttcc cgctgccgcg attgcggagc ttcggctttg ctcgacaagc caatccaggt
     1561 cagccaggcc atcgcaacgg ccatcggaat gtcgtaaaaa ctgccgctat agtaggtgtt
     1621 tcgggcgatg gcccagttgg ccagcgtgga agtggccgca taactcaagc tcatgccgaa
     1681 caagctggca tagagttgtc tccatggacc ctgacttcca atccagcaaa cacacaaacc
     1741 ggcgaggaaa gcgcacttct cggcgttgta aacagcgttg aggttgtggt tatacgcctc
     1801 gacgttggga acgacatatt gccacgggat cactgtcagg acataaagat agaaccacca
     1861 gacgagcagg agagcgaagt cgaggcgccc caggcgggcg gcatactcgt cctggggtac
     1921 gtgcgggcgc aaggccaaag cggccatgag cgggacaatg tgcaggaaca gaatgatgtc
     1981 gcccgcataa agatccggta cgtcgtggcg caagagaact tcgtgataag tccaaagcag
     2041 ctgatagatg aaccagaagg caacgcccag cgcgatcagc gaccagaacc accgcatgcg
     2101 gcctttggaa ctcagaatca gcgggatgaa ggccgccgtt gccgaggcca gcaggaaaca
     2161 ctggacggtg tcgctcagcg cagtgaggcg gaaagactgg tgcagcatga acgtcgctac
     2221 tagttgcacc gataaggcag ccgcaaccac caccaaccag agtttctgcc gagcgctcaa
     2281 aggcacagcc cccggcacca ttctagatgt gatcgtggag cgaaaacggt tactgtagta
     2341 accttagccc actggttcgg gcgtgcggag tccagctttg ccttgctgta agctgttcat
     2401 cttcagtacc atgcagcaca gccgccacgc cggtggagat tgaccccatt gcatcctcaa
     2461 gcggagcgct accgacaatc atggagactg cgaattccgc ccgatcccgg tgaaacgcgg
     2521 cggattgagc gctggctggc gacggcgcgc gtgttcctgg cggtggcaac gctggttgcc
     2581 attcgcatgg acccgaccga actggggcac tcctgggcag cctacgggct tttcgttttc
     2641 tacctggcca acggcattct catcatgatg ctgctccggc gccggcagga gtcgacgccc
     2701 gccttccgcc tcctggttca taccacggac atcatctggc cggcgttgat ctcgatattc
     2761 tccgagggcc ccagaactcc gttctttctg tttttctttt tcgttctggc ggcggctgcc
     2821 taccgctggg gtctgtggga gaccttggga actgcagcgg ccgaggtggc actgctttgg
     2881 gccgagagct atttccttct gcgcgtgcct ctggggccgg gccggacact accctggcaa
     2941 ttcctgagcg gtctgcacgt cagcgtagga acgttcgaac cgcagcgact gttcatgctc
     3001 tcgatctacc tgatcgtgat ggggctgttg ctgggttacc tggcggaaca gcagaaacat
     3061 ctgcgcgccg agaaagctgt cgtgaccggt attctttcgc aagtgcgcgt cgaggccggg
     3121 ctgaccggaa ccatccagca gatctttcgc gagaccctgt caatgtatgg tgcgtcgcat
     3181 cttgttgtgg cttcgcagga aacgcacagc caccgagtct ttctcggcga actgatgaac
     3241 tcggggaatg gcgcgctctc ggaattccgc tggctcgaat cgaagccgcg agatgccaag
     3301 acgtatctcg aagattttcc cggagatgtc tgttacgcca caaccgaccg ggatcgctgg
     3361 tcgattctgg cgctcgatga gaatggcctg gcggtacccg cagtcaacgc cgcaccaatt
     3421 tcccagctgc gtgaggtaca gaatttcaac tccctgatta cagtggcctt tcttttcggc
     3481 agcgactgga gaggccgggt attcctgttc aatcccaact ggcgcggtga aaagcaggaa
     3541 gagttgcgct tcctgctcga cctggtgcgt cagatagggc ctgccatcta caacgtctac
     3601 cttctgcacc gcctgcgccg gcgtgcggga gcggccgaac gcgcccgatt cgcgcgggaa
     3661 ctgcacgatg gcgccgtgca atcgctgatt gcggttgaaa tgcaggtgga cgtcgtgcgg
     3721 cgccaggcag aagcgggcag gaccatcacg ggggaactgg gccgcatcca gggattgttg
     3781 cgcgaggaag tgctcaagct gcgagagtta atgcagcaga tgaaatccat cgatgtagac
     3841 gcgccacgtc tgctcggagt tctgcacgac acggtcgagc gcttccagcg ggagactggc
     3901 atcagcgcac gctttgtcac cgacatcgac aaactcgaca tgccgcaacg ggtgtgccgc
     3961 gaaatgttgc gcatcgtgca ggaggggctg gtcaacgtca ggaagcacag cggggcgcgg
     4021 catgcgctgg tccgcctcgg gttcaatcac gaccaatggc atctgacggt ggaagacgac
     4081 ggcaaaggtt atccattcac agggagactc agtcagacag aaatggaaga gatcggaaag
     4141 ggtccaatga tcatcaagga acgtgttcgt ttgattgctg gcgaactcac ggtagaatca
     4201 aatcccggac aagggacgcg cctcgaagtc accgtaccgc gcagcggaga agtgcctcat
     4261 gaactttaga aagcagacag tgcgcattgt cattgccgac gaccatccga tcttccgcga
     4321 cggtctgcgg cggcttctgg aagcggaggc tgatttgaaa gtaatcggcg aagcctccga
     4381 cggagccgag gccgtgaagc tggcgcggac gctaaaaccc gacattcttc tgctcgatct
     4441 ggctatgccc aagcatccgg gcctggaagc gctgcgcgat ttgagcgtgg cgcccggcgc
     4501 caatccggtg cgcgtgatcc tgctgacggc ggcggcggaa aagagccaga tcgtcgaagc
     4561 cctgcagttg ggcgcgcgcg gagtcgtgct gaaggactcc gccacgcaac ttctgctgaa
     4621 agccattcac accgtgatgt ccggcgaata ttgggtcggc cgggagagcg tatccaacct
     4681 ggtgcagtac ctgcgcacct tgatgcagtc ctccagcgac gaagcccggc agaagaaatt
     4741 cggcctcacc ccacgggaac tggagatcgt ttctgccgtc gtggcggggt actctaacaa
     4801 ggaaatagcg gagtacttca agatcagcga agatacggtg aagcatcacc tgagcaatat
     4861 ctttgacaag ctgggggttt cgaccaggct cgaactggcg ctgtttgcgg taaatcaggc
     4921 cctgccgctc aaaagcattg cctagattgg agatacggcc tggacgagta acgatttgct
     4981 ggcactttgg tgctattggc gggcttgggc cccacagatt atgatttcac aaaacaagac
     5041 cagcctcggg aatatctgcc agactcgcgt gcgagcggca atccccgcct gagcgagtgg
     5101 gagaatattt gtattgtcag ttggaaagaa gggagccccg tggcaagact cttacagcag
     5161 ctctggaacg atgatagcgg ccaggatatc gccgaatacg cggtgatgtt ggccgttatt
     5221 ctggtaatcg tcgtgggtac gattcgactc attgggtcga atgcgaacaa cgtattctcg
     5281 caagtgggca gcgcgattca gtagagcagc aaggcagtag cggcatgtag tctgcaaagc
     5341 gtacttcacg tatgcgttcg cgcgtttctt ccttcacccg gaattctgcc acgcccttgg
     5401 cagcccgtga atccctcggt ttgccacttc ctaaaagcct acttttgctc cggtgctgcc
     5461 ggagtcgaag gagacgtggt ggtgagcgcc accttctccg tcgaacccgt cgacaatcgc
     5521 gtcaggtagg ggaagaacgg cgcaatctcg aagggcatct tctcgcgagc cgaggtcagg
     5581 gctactacct tcgctttgga gagctcaacc tggtcggtcc agggatcaac tttgatccgg
     5641 ccgccgagcg gcagtgctgc gatctgatcg agccgcttgg catcgagcgc cggagcggtt
     5701 cccaccaggc tagccatacg catgctcttg ccttctgcca gactgttgcc cagatgaggc
     5761 cggattaggt acggcagatc gggagacttt tcctgcagtt ccttcaggaa caatcccgcg
     5821 ttgccgagtt tgtccttgta cggggaattc ttgagcaact cgatcgcctt ggcgtcggcg
     5881 gcatcctcat ccgcctggtt gcgtttgaaa tccagccgct gaaatgaatc ttcgtcgggg
     5941 aagaacatct tgtcgttgaa ggcgagcttg gtgtcgaggc gatggccgag cacgatgtgg
     6001 ccgagttcat gggacagaac catagccagc gaagcttcgt cgggaagcac gtcgagcaat
     6061 ccccggctga tgacgatcgt atgaccaatg gtgaacgatt ccagcgggga agtcagcagc
     6121 acacggcaac ggacgtctcc ctgcaaatcg atgttgttgg tgaccaggag attgttgacg
     6181 acggtgcaca gaattttgtc gatgtctccg ggaggcgcca gcaatcccac
//
LOCUS       DASPUL010000254         6866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1821363, whole genome shotgun sequence.
ACCESSION   DASPUL010000254 DASPUL010000000
VERSION     DASPUL010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6866)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1821363"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VEJ00_06345"
     CDS             complement(<1..569)
                     /locus_tag="VEJ00_06345"
                     /inference="COORDINATES: protein motif:HMM:NF015412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00814.1"
                     /translation="MRTFNLCFLGFGNVNRTLVRLLDDRAAELRDKYSITFRITGIAS
                     RSLGWLADPNGLDSATALALGTDREGHGLSRANIAPRKNAASAAEVNAWLAAARADVL
                     FEATSLHVETGQPAIDHIRAALHHGAHAITANKGPVVHAYRELRDLATKQGKKFLFES
                     TVMDGVPIFSLFDQLPAIHLQGFHGILNST"
     gene            complement(770..1168)
                     /locus_tag="VEJ00_06350"
     CDS             complement(770..1168)
                     /locus_tag="VEJ00_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HXX00815.1"
                     /translation="MPQVIPDKYRDLFQKRAFASLGTLMPDGRPQVTPVWCDFDGEHV
                     IFNSAKGRQKDRNVRRDPRVSLAIIDPENPYRYLEIRGRVVEITEDGAAAHIDKMAKK
                     YLGVDKYPYGQPGEVRVLYKIKPEHSTVMG"
     gene            1605..2591
                     /locus_tag="VEJ00_06355"
     CDS             1605..2591
                     /locus_tag="VEJ00_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HXX00816.1"
                     /translation="MATPESQANLDEKEIVMDRQRNGYAGLQRVAALSFVVLMMAAAS
                     LQALAANEAAQKYPPTIVFMTDFGVVDDSVAICRGVMYGIMPTVRIVDLTHQVTPFSI
                     LDGARFLYGATPYFPAGTVFVVVIDPTVGSTRKAIVARSGRGQFFVLPDNGLLTLVEQ
                     RDRIEAVREITNPDWMIGNKLSSTFHGRDIFSPAGAHLARGDDWTTVGPEMAVKDLVR
                     LELKAAKFDEHGLSAMVIATDGPFGNLVTNVDSDDFLKLGYQRGQDVPVTLGEREMKI
                     KFVKTFSDVPLNQPLLYIDSRGRLGVAVNQNSFAATYGIKPPVVLTIPRAGK"
     gene            2674..5913
                     /locus_tag="VEJ00_06360"
     CDS             2674..5913
                     /locus_tag="VEJ00_06360"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXX00817.1"
                     /translation="MVVYVALVLGAVVMASSGLAAQSFRGSIRGKVTDPSGGLVTGAK
                     ITAKSINTGLTRDTASSDEGTFVLAELPAGTYTVTVNATGFAPVAQNVVVNVGLDTTA
                     DFGLLKVEQHQETLTVTEEAPLVEATRDVLGEVVDQRLVADLPLNGRDFGKLVALVPG
                     ATVEPSGVAAIQSGFGQFSINGNRDRSNNYTVDGTDNNDPFFNNSAFNQTGIGGAPAS
                     LLPIDAIQEFNLQADFAPEYGRNSGSVINILTKSGSNHLHGTAFEFLRNSAMDARNYF
                     NRTVDPNTGLPVKKTGFRNNNFGASLGGPIVKDKIFFFGAYEGQRERVGSDFSLLVPT
                     QQQIAQAEADAGGSANVNPALVKILNFFYPNVPSAPASPPAALVPYSVKDKNDGNYLL
                     AKMDLNLRPSESLTARYAYAGNQQTFPLGNPGTLNASRLSQFAQNSPTRVQLFSSSFL
                     STLSSTRVNEIRFGYSRYRTSFTASDDTLDPLALFGINFGTGKLGMPEIDFGGIFDNL
                     GATAFSIPRGRTSQSYQILDNFTWLKGKHTIKVGGEYRRIAISSFNDNLERGAIILPT
                     QNGLDSNPGTDILAAYFLGLTSFTLGDTGNTQRTTYNNGMSFFAQDDFRVTPSFTFNY
                     GIRWEYFGPISEKNNILANLGADGNLAMVGTDGVNGAYERDLNNFGPRFGFAWNVVPR
                     TVVRGAYGIYYDYIPQDLMIANFTNDAGLVTPPLPPKPVLPLSFSQGAFATAPPLNTP
                     PAPVFTAITQGPFDIFFTPRNLVTPYSQNWNLNLEQQVAKEFALQLGYVGSKGTKLVR
                     LRDANQAFVDGSRPNPAYGFMDEFANLTSSVYHGLQATLRTRNWHGLSGFTGYTWSKS
                     IDDASDGINFANTVALPQDSNNLPGERGPSNFDTRHRFTGAFNYVVPDLLGPKRFSRG
                     WQLNTIVTAQSGRPIPLVCSCDTSGTGFPTPSNSLQRPDLVPGQSVINSNWESAPDSL
                     GYLNGNAFVAAPTGTFGTLGRNAIYGPKFWNLDFAVLKDTQLRERLMLQLRLEFFNIF
                     NHPNFALPGFSITPGSNDLITQTPDQAQTNPGLGGGGPRVIQIGAKILF"
     gene            complement(6123..>6866)
                     /locus_tag="VEJ00_06365"
     CDS             complement(6123..>6866)
                     /locus_tag="VEJ00_06365"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HXX00818.1"
                     /translation="LGAFADSLTDSSAVPYSDIPSFPQSTAIGHAGRMVIGKAGDVPV
                     AAMQGRVHQYEGYSAQQAAFPIRVFARVGVKAVILTNAAGGISLGYSQGALVLIRDHI
                     NLQGANPLVGPNDDRFGVRFPDMTHAYSRAYREAAREEAAKLGIPLHEGIYAALLGPS
                     YETPAEIEYLRRIGADLVGMSTVAEVIAARHMDLDVLAISCVTNMAAGILDQPLSHAE
                     VMETGERVKTTFEALLRAVLPRLAQDVGH"
BASE COUNT         1397 a         1912 c         2201 g         1356 t
ORIGIN      
        1 gtggagttga ggatgccgtg gaatccctgc aggtgaatgg cggggagctg gtcgaaaaga
       61 gaaaagatgg ggacgccgtc catgacggtg gattcgaaca ggaatttctt gccctgcttc
      121 gtggcgagat cgcgcagctc gcggtaggcg tggacgacgg gtcctttgtt ggcggtgata
      181 gcgtgggctc cgtggtggag tgcggcgcgg atgtggtcga tggcgggctg gccggtttcg
      241 acgtgcaggg aggtggcttc gaagaggacg tcggcgcggg cggcggcgag ccaggcgttc
      301 acctcagcgg ctgaagccgc atttttccta ggcgcgatat tggcacggct taagccgtgc
      361 ccctcccggt cggtgcccaa agcaagagcc gtggcggaat ccagtccgtt cgggtcggcg
      421 agccagccca ggctgcgcga ggcgatgccg gtgatgcgga aggtgatgct gtatttgtcg
      481 cgcagttcgg cggcgcggtc gtcgagcagg cgaacgagag tgcggttgac gttgccgaag
      541 ccgaggaagc aaaggttgaa ggtgcggatg gcggattcct tacacggggg ctaaagcccc
      601 gtccctaatt tgccttgacg cggcgctaaa gcgccgctct tccacggctg cataaactgt
      661 tgagcagaaa tcagaggttc cccttcacag cagctccggc acgccgtcgc ttaggacagg
      721 tccttcactc gcgcaagagc ggcgctggct caggatgaca agccttgctt tatcccatca
      781 ctgtgctgtg ctcgggcttg atcttgtaga gcacgcggac ttcgccgggt tggccgtagg
      841 gatacttgtc gacgccgaga tatttcttgg ccatcttgtc gatgtgggcg gcggcgccgt
      901 cctcggtgat ctcgaccacg cggccgcgaa tttcgagata gcgataggga ttttcgggat
      961 cgatgatggc gagcgagacg cgcgggtcgc ggcggacgtt gcggtccttc tgacgtcctt
     1021 tggcggagtt gaagatgacg tgttcgccgt caaagtcgca ccacacgggt gtgacctgcg
     1081 ggcggccgtc gggcatgagc gtgcccaggc tggcgaatgc gcgcttctga aataaatcgc
     1141 gatacttgtc gggaatgact tgcggcatta gagcctccac gatggggtag cagcagggga
     1201 attgcagcag cattatagac cgcggacggg atgggcgttc ggaaatgcac tggggagaaa
     1261 tcgcagaacg gcaactgcaa cttcaggcag aacgaaaatg cttgcgcggc aatgcgcgcg
     1321 agatcgctcg ccgctcgggc gaaagcgcgc cggcttcggg atgacgccac aacggcacag
     1381 attcgctccg ctcgcgacgg cttcccgttt ttcctcatct ttgattagaa ttgggacctt
     1441 actggaattc agcagagttt gcctgttgcg gcagaacacg acgtgagcga gtgggctgcg
     1501 gcggtgaaag agcttgaccg cgaaggtcgc aaagaacctc cgcaaagggc cccaagaaaa
     1561 ccagcctgag ggccgcgccc tgatgcggaa ccgacggcgg agcaatggcg actccggaat
     1621 cacaggctaa tcttgacgaa aaggaaatcg tgatggatcg gcagcgaaac ggttacgccg
     1681 gactccagcg ggttgcggca ttgagcttcg tcgtattgat gatggccgcg gcctccttgc
     1741 aggcgctggc agcgaacgaa gcggcgcaga agtatccgcc cacaattgtc ttcatgaccg
     1801 acttcggggt ggtcgacgat tctgtggcca tctgccgcgg agtgatgtac ggcatcatgc
     1861 ccacggtgcg catcgtggat ttgacgcatc aggtcacgcc gttttcgatt ctggacggag
     1921 cgcggtttct gtatggggcg acaccgtact tccccgcggg gacggtattc gtggtggtga
     1981 tcgacccgac ggtggggagc acgcgcaaag cgattgtggc gcgatcgggg cgcgggcaat
     2041 tctttgtgct gccggataac gggctgctca cgctggtgga gcagcgcgac cggatcgaag
     2101 ccgtgcggga gatcaccaat cctgactgga tgatcgggaa caagctgtcg tcgacgtttc
     2161 acgggcgcga cattttttcg ccggccgggg cgcatctggc gcggggcgat gattggacca
     2221 cggttggtcc cgagatggcc gtgaaagatc tggtgaggct ggagctgaag gcagcgaagt
     2281 tcgacgagca cgggctgagc gcgatggtca tcgcgaccga tggtccgttc ggaaatctcg
     2341 tcacgaatgt tgactcggac gattttctga agctgggtta tcagcgcggg caggatgttc
     2401 ccgtcacgct gggcgagcgg gagatgaaga tcaagttcgt caagacgttc agcgacgtgc
     2461 ccctgaacca gcctctgctc tacattgact cgcgcggacg tctgggagtg gcagtgaatc
     2521 agaacagttt cgccgcgacg tatggcatca agccgccggt ggtgttgacg attccacggg
     2581 cgggcaagta agggcgcagc caggccgttc accacggctg cgcgtgcaag ggggcgtgat
     2641 atgaatcggg taaattcgtc tttgggtcgc aggatggtcg tatacgtggc tctggtgctg
     2701 ggcgcggttg tgatggcgtc gagcggactc gcagcgcaga gttttcgggg atcgattcgc
     2761 gggaaggtga cggacccgag cggcggtctg gtcaccggcg cgaagattac cgcgaaatcg
     2821 attaacaccg gcttgacacg tgacaccgcc agctcggatg aaggaacctt cgtactggcg
     2881 gaactaccgg cgggaacgta cacagtcacg gtgaacgcca cgggattcgc gccggtggcg
     2941 cagaacgtgg tggtcaacgt tgggctggac acgactgccg acttcggcct gctgaaggtg
     3001 gagcagcatc aggagactct tacggtcacg gaagaggcgc ccctggtcga ggcaacgcga
     3061 gatgtgttag gggaagtggt ggaccagagg ctggtggcgg atcttcccct gaatgggcgg
     3121 gattttggaa agctggtcgc actggtgccc ggagcgacgg tggaaccgtc gggcgtggcc
     3181 gcgattcaga gcgggtttgg gcagttctcg atcaacggca atcgcgaccg ctcgaacaac
     3241 tacacggtcg acggcacgga caataacgat ccgttcttca acaactcagc gttcaaccag
     3301 acagggattg gcggcgcgcc ggcgtcgctg ctgccgatcg acgcaattca ggagttcaac
     3361 ctgcaggcgg atttcgctcc ggaatatggg agaaacagcg ggtcggtgat caatatcctg
     3421 accaagtccg ggagcaatca cctgcatggg acagcgtttg aattcctgcg caacagcgcg
     3481 atggatgcgc gcaattactt taacaggacg gttgacccta acaccggtct gcccgtcaaa
     3541 aagacaggtt ttcgcaacaa taacttcggc gcctcgctgg gcggcccgat cgtcaaggac
     3601 aagatcttct tctttggggc gtatgaaggg cagcgggaaa gggtaggatc tgacttctcc
     3661 ctcctggttc ccacacaaca gcaaatcgcg caggcagaag ccgatgcggg cgggagcgcg
     3721 aacgtcaacc ctgccctggt gaagatactg aatttcttct atccgaacgt tcccagtgcg
     3781 cctgccagcc cgccggctgc cctcgtgccc tacagtgtaa aggacaagaa cgatggcaat
     3841 tacctgctgg cgaagatgga tttgaattta cgcccttcgg aaagtctcac agctcgttat
     3901 gcctacgcgg gcaatcagca gacgttccca ctcggaaacc cgggaacctt gaatgcgtcc
     3961 aggctgtcgc aatttgcgca gaactcgcct acaagggtgc agctattctc cagcagcttc
     4021 ttatcgaccc tgagcagtac gcgggtcaac gagattcgtt tcggctacag ccgatacagg
     4081 acttcgttca cggcctccga tgacacgctt gatcccctgg cgctgtttgg aattaatttc
     4141 ggaaccggca agctgggcat gccggagatt gacttcggcg gcatcttcga caacctgggc
     4201 gcaaccgcat tcagcattcc ccgcgggcgc accagccaga gttatcagat tctcgacaac
     4261 ttcacgtggc tcaagggaaa gcacacgatc aaagttgggg gcgaataccg gcgtattgcc
     4321 atctccagct tcaacgacaa cctagagcgc ggagcgatca ttcttccgac gcaaaacgga
     4381 ctggacagca atccgggaac agatattctg gcagcctatt tcctgggtct tacatccttt
     4441 acgctcggag atacgggcaa cacacagcga actacctaca acaacgggat gagctttttc
     4501 gcgcaggatg atttccgcgt aacccccagc tttaccttca attacgggat tcggtgggag
     4561 tatttcgggc cgatcagcga aaagaacaac attctggcca acctgggagc cgacggcaat
     4621 ctggctatgg taggcaccga cggcgtgaat ggggcgtatg aacgcgatct caacaatttc
     4681 gggccgcgtt tcggcttcgc ctggaatgta gtcccgcgaa cagtagtgcg tggagcatat
     4741 ggcatttatt acgactacat tccgcaggac ctgatgattg cgaatttcac taacgatgcc
     4801 ggtctggtga cgccaccgct gccaccgaag ccagtcctgc ccctcagctt cagtcaaggc
     4861 gcattcgcga cggctcctcc cctgaacacg ccgcccgcgc cggtattcac ggcgattacg
     4921 cagggacctt tcgatatctt ttttacaccg cgaaacctgg tcacacccta ctcgcaaaac
     4981 tggaacctca acctggagca gcaagtggcg aaggagtttg ccttgcagct gggatatgtt
     5041 ggaagcaagg gaaccaaact ggtccgcctg cgcgatgcga atcaggcctt cgtcgatggc
     5101 agccgtccga atcctgcata tgggtttatg gatgaattcg caaacctcac atcgtcggtt
     5161 tatcacgggt tgcaggcaac gttgcggacg cggaactggc acgggctcag cggattcacg
     5221 ggttacacct ggtcaaaatc cattgacgac gcctccgacg gcatcaattt cgcgaacacg
     5281 gtggcgctgc ctcaggactc gaacaatctc ccgggcgagc gcgggccttc caacttcgac
     5341 acccgccacc gtttcacagg cgcgttcaac tacgtcgtcc ctgacctgtt gggtcccaag
     5401 cgcttttctc ggggatggca gctaaacacg atcgtcacgg cccaaagcgg tcgccccatt
     5461 cctcttgtat gctcttgtga taccagcggc acaggattcc cgacgccaag taacagcctg
     5521 caacgtcccg atctggtgcc ggggcagtct gtcatcaatt cgaactggga gtcagcaccc
     5581 gattcgctgg gttatctgaa cggtaatgcc ttcgtggctg ctccgacggg gactttcgga
     5641 acgctgggac gcaacgcgat ctatgggcct aagttctgga atctggactt cgcggttctg
     5701 aaggatacgc agctccgcga gcggttgatg cttcagttgc gcctcgagtt cttcaacata
     5761 ttcaaccatc cgaacttcgc gcttccgggt ttctccatca ctcccggaag caacgatctg
     5821 atcacgcaga cgccggatca ggcgcagacc aacccaggct tgggcggcgg ggggccgcgg
     5881 gtgatccaga tcggggcgaa gattttgttc tagtcggggt tgagcatccc cacttctcgc
     5941 gcacagaacg cgcgagaaat ggggcaccca tcgcctacac gcggccctca gcggctgaag
     6001 ccgcccaaac cgtggcttgc ttacggcacg ccagaagtcg tgcctttccc gtgtgtccct
     6061 cccacagccg aggagcgcta caagaacatt gcgggctcac tgcgagaccc gtaagaatca
     6121 aatcaatgcc caacatcctg cgccaggcgc gggaggaccg ctcgcagcaa cgcctcgaag
     6181 gtcgtcttga cgcgctcgcc ggtttccatc acttcggcgt gggagagcgg ctggtcgaga
     6241 atgccggccg ccatgtttgt aacgcacgag atcgcgagga cgtccaggtc catgtggcgg
     6301 gcggcgatga cttccgcgac cgtcgacatt ccgaccagat cggcgccgat gcggcgcagg
     6361 tactcgatct ccgcgggcgt ttcatagctc ggcccgagca gtgcggcgta gattccttcg
     6421 tgcaatggaa tgccgagctt ggcagcttct tcgcgagcgg cttcgcggta ggcgcgggag
     6481 taggcgtgcg tcatgtcggg aaagcggacg ccaaagcggt cgtcgttcgg cccgacgagc
     6541 ggatttgcac cttgcagatt gatatggtcg cggatcagaa cgagcgcgcc ttgcgaatag
     6601 ccaaggctga tgccgccggc ggcgttggtc aggatcacgg ctttaactcc cactcgcgcg
     6661 aaaacgcgaa tgggaaatgc ggcctgctgg gcggagtagc cttcgtattg atgaacgcgg
     6721 ccctgcatgg cggcgacggg tacgtctccg gctttgccga tgaccatgcg tccggcgtga
     6781 ccaattgcgg tcgattgcgg gaaggagggg atgtctgagt aaggcaccgc ggatgaatcg
     6841 gtgagcgagt ccgcgaaagc gccgag
//
LOCUS       DASPUL010000255         4124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1272751, whole genome shotgun sequence.
ACCESSION   DASPUL010000255 DASPUL010000000
VERSION     DASPUL010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272751"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VEJ00_06370"
     CDS             <1..228
                     /locus_tag="VEJ00_06370"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HXX00819.1"
                     /translation="VLSIGGVEAAAFFRELPDGRYRVSLRSKGKLDVAHVAESFGGGG
                     HECASGCSLDGPLAEAVRQVLMQLRRGPSVQ"
     gene            335..1945
                     /locus_tag="VEJ00_06375"
     CDS             335..1945
                     /locus_tag="VEJ00_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXX00820.1"
                     /translation="MTLRTRTDVLLIAGFCAFLFFYGVGQFGLIGADEPRYAQVAREM
                     LERHDWVTPTLGGRPWLEKPPLCYWQAMAVYSVFGVSDTAARLPSSVDATLLVIAVYV
                     FFRRFRRGVELDAALITASCAGVIGYARAASMDMALAVTFAIGMLGWWAWRESEKRIY
                     LALFYGCIALGTLAKGPVAPLLAAVVIVLFATMTRELRLVGRTLWPSGILLFCAIALP
                     WYVAVQVRNPQFFREFILQHNLARFSTNLYHHPEPFWYYLPVMLIALLPWIVFVVAGA
                     AGTLQAWWSERKSSAPSPDFELHFNVFASLWLVVPVLFFTLSQSKLPGYILPTVPAGA
                     ILLAEYLRHHADHEQRVAKWLAVLHALVAVAPIVPAALVAYLVTQHRLPSGRPLLLTL
                     AVALVLCAGIALTLVSRLGLRMLRFVTLIPVVLAVAAVLKLGTTSIDQELSARPLAWE
                     LVRLETHRLPMAVCGTSRELEFGLAFYRNQTIARYESGTVPTEEHLLVAPPSWKPDVA
                     SRTVGRRLSFLGHYAPQDVDYYWVAALK"
     gene            2020..2358
                     /locus_tag="VEJ00_06380"
     CDS             2020..2358
                     /locus_tag="VEJ00_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00821.1"
                     /translation="MFRRELLTRLPQALGLGLLGLSRAGAQETAGHKTLKIMIKSAWG
                     SDDPTRASFPFVHGLALAEAGHEVQIFLTGEATYLMRKATVAQIFAVGWPPLSELQQK
                     VVAKHIQVFS"
     gene            complement(2557..3144)
                     /locus_tag="VEJ00_06385"
     CDS             complement(2557..3144)
                     /locus_tag="VEJ00_06385"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXX00822.1"
                     /translation="MRLTEIRIAGFGGQGVILSAIVLGKAASIYENGFATMTQNFGPE
                     ARGGACSAQLVVSDSPVLYPYTTRPDIMVIMSQEAYNRFAHELKPGGTLIIEEDLVRV
                     ADLKGDPKVYAIPATRFGEELGKRMVLNSVMVGFFTAVTKLLDPEAVRKAVADSVPPN
                     FRELNLKAFEKGFEYGTTAKAGSPPELEEVAYAKE"
     gene            complement(3135..4031)
                     /locus_tag="VEJ00_06390"
     CDS             complement(3135..4031)
                     /locus_tag="VEJ00_06390"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HXX00823.1"
                     /translation="MLNVLADDTVNPVEPFLRTDRMPHIWCPGCGIGTTVNSFSRALI
                     ESKIDLESLALVSGIGCTGRVAGYVKLDSFHTTHGRAIPFATGLKLANPKLNVVVYSG
                     DGDLSAIGGNHLIHAARRNLDLKVICVNNLIYAMTGGQTAPTTPGHATTSTNPYGTFE
                     PAFNLPHLVDAAGAVYVARWTTFHVRQLSRAISEAFQKKGFSFIEVISPCPTLYQRRN
                     KMGDGLDTMKYYKEKSKVKNGAPTSEVGLTLNGEIAVGKFIDRDRPDYQTMMRAQYVE
                     SLGERYVEMADPEEPEMEYVCD"
BASE COUNT          729 a         1188 c         1276 g          931 t
ORIGIN      
        1 gttctctcca tcggcggcgt cgaggcggca gcgttctttc gcgaactgcc cgacggacga
       61 tatcgcgtga gtctccgcag caagggaaaa ctcgacgtgg cccacgttgc cgagagtttt
      121 ggcggcggcg gccacgaatg cgccagcggc tgctcgctcg acggtccgct cgccgaggcc
      181 gttcgccagg tgctgatgca actgcgcagg ggaccctccg tacagtagcg acccgattgc
      241 agatttgaga tttcagattg cagatgaaaa acctgcgtct gtaatttcct tggtttttga
      301 tctgaaatct gaaatcttac ttctgaaatt tcccatgact cttcgcaccc gcacggacgt
      361 gttgctgatc gccggcttct gtgcatttct gtttttttat ggcgtgggcc agttcggact
      421 gatcggggcc gatgaaccac gctacgcgca ggtggcgcgc gaaatgcttg agcgccatga
      481 ttgggtcaca ccgacactcg gaggacgtcc ctggctggag aagcccccgc tctgctactg
      541 gcaggcgatg gcggtctact ctgtcttcgg cgtgagcgac accgcggcac ggctcccatc
      601 atccgtcgac gctactctcc tcgtcatcgc tgtttacgtt ttctttcggc gatttcgtcg
      661 cggtgtcgag cttgatgccg cgttgatcac ggcatcatgc gcgggagtga ttggctatgc
      721 ccgagcggca tcgatggata tggcgctggc tgtgacgttt gcgatcggga tgcttgggtg
      781 gtgggcctgg cgtgagagcg agaagcgaat ctacctcgca ctgttttacg gttgcattgc
      841 gctgggtacg ctggcaaaag ggccagtggc gccactgctc gccgcggtgg tgattgtgct
      901 gtttgcgacg atgacgcgtg agctacggct tgtgggcaga actctgtggc catccggcat
      961 tctgctgttc tgcgcgatag cactgccctg gtacgttgcg gtgcaggtgc gtaatccgca
     1021 attctttcgc gaatttatct tgcaacacaa tctcgcccgt ttctcaacga atctctacca
     1081 ccatcccgag cccttctggt attacctccc ggtcatgctg atagcactgc tgccgtggat
     1141 cgtctttgtg gtagcgggcg ccgccgggac gctccaagcc tggtggagcg agagaaaatc
     1201 gagcgccccg tctcccgatt tcgaattgca tttcaatgtg tttgccagcc tctggctagt
     1261 cgtgccggtt ctgttcttca cgctatcgca gtcgaaactg ccgggataca tacttcccac
     1321 ggtgcctgcg ggagcgattc tgctggcgga atatcttcgc caccatgccg accacgagca
     1381 aagagtggcg aagtggctgg ccgtgctgca cgcgcttgtc gcggtcgccc cgattgttcc
     1441 ggcggccctg gtcgcatacc tggtcacgca gcatcgcctg ccgtcgggaa ggcctctgct
     1501 gttgacgctc gccgttgcgc ttgtgctttg cgccggcatc gcgctcacac tggtcagccg
     1561 gcttggcctg cgaatgctgc gcttcgtcac gctgataccg gtggtcctgg cggtggcggc
     1621 agtgttgaag ctaggaacga cttcgatcga tcaggagctt tcggcgcgtc cgctggcctg
     1681 ggaactggtt cgcctcgaaa cccaccgctt gccgatggcg gtatgcggga catcgcgcga
     1741 actggaattc ggcctcgcgt tctatcgcaa tcagacgatt gcgcgctacg agtccggcac
     1801 ggttccgacg gaagagcatc tgctggtcgc accgcccagt tggaagccgg acgtcgcatc
     1861 ccgaacggtt ggacggcgct tgtcgttcct cggccactac gcgccgcagg atgtggacta
     1921 ctactgggtc gcggcgctga aataatccac ctttaccccc ttttcgagcc ggtgtacatt
     1981 cttccgcaat gttcacttcg tggatttcag gaggacctca tgttccgacg agaacttctc
     2041 accagactcc cgcaagcact gggcttggga ctgcttgggc tgagccgggc gggcgcgcaa
     2101 gagacggcgg ggcacaagac gctcaagatc atgatcaaga gcgcgtgggg ttccgatgat
     2161 ccgacccgcg cgtcatttcc attcgtccac ggcctggcac tggctgaggc cggccatgag
     2221 gtacagatct tccttaccgg ggaggctacg tatctcatgc gcaaggctac cgtcgcgcag
     2281 attttcgcgg tcggctggcc tcctctgagc gagttgcagc agaaggtcgt ggccaaacac
     2341 atccaggtat tttcttgagg ggcctgctcc cgggcccgtg gggtcacgga aacagatttg
     2401 caagattggc gggcaaagtg gggcagtccc gctgtttttg tctccctggt ggaatgggcg
     2461 gaccgaatca tcacggagta gctgacagag ttggggaggg ccgcgactcg cgagtgcaac
     2521 gggcgatcga gaaatctccg gaagaaggag tatgtctcac tccttcgcgt acgccacttc
     2581 ttccagttcc ggcggagagc cggctttagc cgttgtgccg tactcgaatc ccttctcgaa
     2641 cgccttgagg ttcagttcac ggaagttcgg cggaacggag tcggctacgg ctttgcgtac
     2701 ggcctcggga tcgaggagtt tcgtcactgc ggtaaagaat ccgaccatca ccgaattcag
     2761 aaccatacgc ttgcctaact cttcgccgaa gcgagtggct gggatcgcgt aaaccttggg
     2821 gtcgcccttg agatcggcga cgcgaaccag atcttcttcg atgatgagcg tgcctccggg
     2881 cttcagttca tgcgcgaagc ggttgtaggc ctcctgcgac atgatcacca tgatgtcggg
     2941 ccgggtcgtg tagggataga gcacaggcga gtcggaaacg acgagttgcg cgctgcaggc
     3001 cccgccgcgg gcttcggggc cgaagttttg cgtcatggtg gcgaagccgt tttcgtaaat
     3061 cgacgccgcc ttccccagca cgatagccga gaggattact ccctggccgc caaaacctgc
     3121 aatgcgaatc tcggtcagtc gcatacgtac tccatttctg gttcttccgg gtctgccatc
     3181 tcaacgtagc gctcgcccag cgattcaacg tactgcgcgc gcatcatggt ttgataatcg
     3241 ggacggtcgc ggtcgatgaa ttttccgact gcgatttcgc cgttcaacgt gagtccgacc
     3301 tcgctggtgg gcgcaccgtt tttcaccttg ctcttctctt tgtagtactt catggtgtcg
     3361 agcccgtcgc ccattttgtt gcgtcgctgg tagagcgtgg ggcagggcga gatgacttcg
     3421 atgaaggaga atcctttctt ctggaacgct tcgctgatag cgcgagagag ctggcgcacg
     3481 tggaaggttg tccagcgtgc gacgtagaca gcgcctgctg cgtccaccag atgcggcagg
     3541 ttgaacgcag gctcgaacgt gccgtaagga ttggttgagg tcgtcgcgtg gcctggagtg
     3601 gtgggtgcag tctgcccgcc ggtcatggcg tagatcaggt tgttgacgca gatgaccttg
     3661 aggtccagat tgcgccgggc agcgtggatt agatgattgc cgccgatcgc cgaaagatcg
     3721 ccgtcgccgg agtatacgac aacgttgagc ttggggttcg ccagcttcag accagtagca
     3781 aacgggatcg cgcggccgtg cgtagtgtgg aacgaatcga gcttcacata acccgccacg
     3841 cggcccgtac aaccgatacc cgagaccagc gccagtgact ccagatcgat cttcgattcg
     3901 atcaatgcgc gcgagaaaga attgaccgtg gtgccgatgc cgcatcccgg gcaccagatg
     3961 tgaggcatgc gatccgttcg caggaacggt tcaaccggat ttacagtgtc gtccgcaagt
     4021 acgttcagca tcggctggcc tccacgatcg ctttcaggat ttcttcggga tggtgaacac
     4081 cacctccggc atgcggaacg gcgaaggtct tcgcactccc ggct
//
LOCUS       DASPUL010000256         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1547585, whole genome shotgun sequence.
ACCESSION   DASPUL010000256 DASPUL010000000
VERSION     DASPUL010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1547585"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VEJ00_06395"
     CDS             <1..894
                     /locus_tag="VEJ00_06395"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXX00824.1"
                     /translation="TTGFNKDLSVDHAFMAEHCRWLLDNGCTGIVTLGSLGEGATLSF
                     DEKLAILRTCVRAVDRRGPVVASISALSTSEAVALAKAAADLGCDGLMVLPPYVYQGD
                     WREMKTHVAAVFNATVLSCMLYNNPVAYGTDFLPEQICELAAEYQNFKSVKESSTDAR
                     RVSAIRALLGHRLEISVGVDDAVLEAIGVGATGWVAGLANALPRESVDLFHLGMNGDG
                     EKAFDLYRWFLPLLRMDTVPNFVQLIKLVQAEVGRGNAGVRPPRLELVGDELEQARKT
                     IHEALRTRPQPANAYALPNRK"
     gene            898..>2111
                     /locus_tag="VEJ00_06400"
     CDS             898..>2111
                     /locus_tag="VEJ00_06400"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXX00825.1"
                     /translation="MLASTVKLTGRSIIGFREGGGSGELLYATNPTTGHRLEPGFIPA
                     TSEEVDLSARLAAEAFDVYRRVSGRDRGAFLRAIATKIEAITGEIVERAGQETALPAG
                     RLQAETARTCAQLRLFAQVAEEGSWVNARIDQAEPDRKPIPKPDIRSMLHPLGPVVVF
                     GASNFPLAFSVAGGDTASALAGGNTVIVKAHAAHPGTSELVGRAVQEAVRECGLPEGV
                     FSVLFGSGAQIGTALMKHPLVKAGGFTGSRTAGRTLMDVAAARPEPIPFYAEMSSTNP
                     VFILPGALRERGDQIAAGLHTSFTLGAGQFCTKPGMVFLAKGAEAAAFADKLRQLVAA
                     SAPFHLLTRTIQSSYDSAISRRKVEAEAKLTACGSQQTPAAGFAVSSAVFETDVASFL
                     GSELGAEIFGPTT"
BASE COUNT          406 a          610 c          665 g          430 t
ORIGIN      
        1 acgacgggct tcaataaaga cctcagtgtc gatcatgcct tcatggcaga acactgccgt
       61 tggttgttgg ataacggctg cacaggcatt gtcactctgg gatccttggg cgaaggcgca
      121 accttatcgt tcgacgaaaa actggcaatt ctgcgcactt gtgtcagggc agtcgaccgg
      181 cgtggaccag tggttgcgtc gatctctgcc ctcagcacct cggaggcggt ggccctggcg
      241 aaagccgcgg cggacttagg ctgcgacggt ctgatggtgt tgcctcccta cgtgtatcag
      301 ggcgactggc gcgagatgaa gacccacgtc gccgctgtat tcaacgcaac cgtactttcc
      361 tgcatgctgt acaacaaccc cgtcgcatat ggaaccgatt tccttcccga gcagatctgc
      421 gaactggcgg cggaatatca gaacttcaaa tcggtaaagg agtcgagcac ggatgccagg
      481 cgagtatcgg ccatccgtgc attgctgggc catcgtctcg aaatatcagt cggggtagac
      541 gatgccgtat tggaagctat cggagtagga gcaaccggat gggttgccgg cctggcaaat
      601 gcacttccgc gagaatcggt ggatctcttc cacctgggga tgaacgggga tggcgagaag
      661 gccttcgatc tgtatcgctg gttcctgccc ctgctccgga tggacaccgt tcccaacttc
      721 gtgcaactca tcaaactcgt gcaggcagag gtaggtcggg gcaatgcggg tgtgcgtccg
      781 ccacgtctcg aattggtggg tgatgagctt gagcaagcca ggaagactat tcatgaagca
      841 ttgcgcaccc ggccgcaacc ggcgaatgcg tatgcattgc cgaacaggaa gtgatctatg
      901 ctagcgtcca cagtaaagct taccggtcgt tcgattattg gattccgcga gggcgggggt
      961 tccggggaac tgctgtacgc caccaatccg accactggtc accgcctgga accgggcttc
     1021 attccggcga cttcggaaga agtggatctt tccgcccgcc tggcggccga ggcgtttgac
     1081 gtctaccggc gagtttccgg acgcgatcgc ggcgcattct tgcgcgcgat cgcgacgaaa
     1141 atagaggcga tcaccggcga gatcgtcgag cgtgccggac aagagacagc gctgccggcc
     1201 ggtcgcctgc aggccgaaac cgcgcggact tgcgcccagc ttcgcctgtt cgctcaagtt
     1261 gcagaagagg gatcatgggt caacgcgcgc atcgaccagg ctgagcctga tcgcaagcca
     1321 atccccaagc ctgacatccg gtctatgttg catccgctgg gacctgtagt cgtgtttggc
     1381 gccagcaact ttcctttggc attttccgtt gccggcgggg acacagcctc ggcgctcgct
     1441 ggcggcaata ctgtgatcgt gaaggcgcac gcggcgcatc ccggcaccag cgaactggtt
     1501 ggtcgcgcgg ttcaggaggc agtgcgggaa tgcggtctgc cggagggagt gttttcggtg
     1561 ctcttcggga gcggtgcgca gattgggacc gcgttgatga agcatccctt ggtgaaggcc
     1621 gggggcttca ccggatcacg cacggcagga agaaccctga tggacgtggc ggccgcgcgt
     1681 cctgagccga ttccgttcta cgccgagatg agcagcacaa atccggtatt tattctcccc
     1741 ggcgcattgc gcgagcgcgg tgatcagatc gcagccgggc tgcacacttc attcaccctg
     1801 ggcgccggtc agttctgcac gaaacccgga atggtatttc tggccaaggg tgcggaggca
     1861 gcggcatttg cggacaagct acggcagctg gttgcggcgt cggctccatt ccatcttctg
     1921 acgcgcacca tccagtcctc ttacgattcg gcgatctcca ggcgcaaagt ggaggccgag
     1981 gccaagctca cggcctgcgg ctcccagcaa acacctgccg caggctttgc cgtaagttcc
     2041 gctgtgtttg aaacggatgt cgcttccttt ctgggctcgg aattgggcgc agagatcttc
     2101 ggccccacaa c
//
LOCUS       DASPUL010000257         5693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1959042, whole genome shotgun sequence.
ACCESSION   DASPUL010000257 DASPUL010000000
VERSION     DASPUL010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1959042"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VEJ00_06405"
     CDS             <1..1324
                     /locus_tag="VEJ00_06405"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXX00826.1"
                     /translation="YGSHKASYANSVPEKRKVKYAIVDTSKVEAGVQLTQDDLREYYD
                     QHRNEYRVPDQVKVSHIWIKMPLPGDDGKVDEKKVAEAQQRADDLSRQLKNGGKFDEL
                     AKKYSEDSGSANVGGSLGWIGRGQMTPEFEKAAYSLSKGQISDVVKSTDGFHIIRLDD
                     RQEAHVKTLAEVKDQIEPIVKHQRAQQIAQRQADSLLRQAQSKSVGLDAAAGAMSIPV
                     ITSDFFSRKDMLPGLGPAPQFMDAVFTLAENSPPEMASASQGIVVFQLLAIKPAATPA
                     FEEIRSRVEDEFKSERSSVLLQQKVQELSDRAKAEHDLKRAAKELGATIKTSDFVLPD
                     GQVPDVGSMTGQASVAFTMKPGEISGPITSGANAVVISVLEDQKPNEADYAAKRDQIR
                     DTLLQNKQQELFGLFVSNLRDQMEKSGKIKINQEEMKNLTRTGSEQGM"
     gene            1336..2907
                     /gene="malQ"
                     /locus_tag="VEJ00_06410"
     CDS             1336..2907
                     /gene="malQ"
                     /locus_tag="VEJ00_06410"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HXX00827.1"
                     /translation="MSFPRAAGILLHPTSLPSRGGIGDFGPAAYQFVEFLASARQGLW
                     QVLPLGPVGYGNSPYSSTSAFAGNPLLISLERLAERGWIDRSKLDSANPDSANPDSQM
                     ADTSAGSRPVEYDQVFAQKMPVLFEAARNLLRAGSSSALDRLKTFQTENAWWLDDFVL
                     FDALRARHKLGSWNQWPHDLAYRDPSALAKARAELAADTQIRSALQFAFYEQWRALRR
                     YCAERAIRIVGDVAIFVNYDSADVWSHRELFRLNEDLQPEVVSGVPPDFFSKTGQRWG
                     NPLYRWDVMKERGYAWWIQRLRWATQHCDYIRLDHFRGFDQFWEIPAGEATAVNGRWV
                     DGPRDDLFAKLHEALGGLPFFAEDLGYITPEVHALRDRLQIPGMAVLQFGFGDEGAHI
                     YLPHRAAGKVIYTGTHDNDTIVGWWHSGASEHERRNAEAYLGRSDDGVHWAFIRAAQC
                     SPASMSVIPLQDVLGLGSEARMNTPSVYGGNWRWRLDATQLRGELAEKLARLAEMTDR
                     LPQAIAVPADDGFVA"
     gene            2999..4390
                     /locus_tag="VEJ00_06415"
     CDS             2999..4390
                     /locus_tag="VEJ00_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HXX00828.1"
                     /translation="MNEAIIEAVAIEKSYPQPDGTRIQVISPTNLVIEPGKIIALLGP
                     SGCGKSTLLRMLTGLSQPSSGMLAWHGKPLNGQMPNVAIVFQSFALFPWLTVLENVEA
                     PLEARGVAAVERHKRALRTLDTVGLDGFETAYPKELSGGMKQRVGFARALVVEPEVLF
                     MDEPFSALDVLTAENLRSELLELWLNKKMPTSAIFIVTHNIEEAVMLADRVMVLGRNP
                     ARIRSDFNVRLKHPRDRKSPRFVELVDYIYKVMTEPEVEHALPDAETTAEIILPKAGL
                     KKQEAPVRTARYQMLPHARVGGIAGLLELLHDRGGREDLFRLSEELVMDVEDLLPILE
                     ACVLLGFALLKEGDVQITPQGIRFADADIQQRKVLFRQAALEHVTILKQIDSILRRKS
                     DHSIADEFFHDILDEHFAEDEVQRQFETAMNWGRYAEIFDYDRENGRLVLTEPAASDA
                     VPVSQNGPSAPES"
     gene            4407..>5693
                     /locus_tag="VEJ00_06420"
     CDS             4407..>5693
                     /locus_tag="VEJ00_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXX00829.1"
                     /translation="MTHTRKTGGLRGFSAYQLLTQLPRVEISYLPDLLMFGVGIAVFY
                     GVLVVGRTWLGPFTPEVEISRSPLALPAYAGYSLLRITIAYVLSLAFTLVYGYIAAYN
                     PRAERFMIPLLDVLQSIPVLSFLPGVMLAMVALFPGRQLGVEAGAILLIFTGQVWNMA
                     FSFYASLKSIPSEMREAAKVYGFSWWQRFVEMELPFSAIGLVWNSMMSVAGGWFFLMA
                     CEMFVLGSRDFRLPGLGSYLQTAASAGDTPSILWGVGTMIAVIVLLDQFFWRPVIAWA
                     EKFKVEQVESTEAPRSWVLDLIHHSRSLARIRQNTFSPLGERLMLHFSHKDSSGKAGL
                     QSAWKTWLARLLGLALLAGMGYGVVRVAIILSGLQKAELQEAGIGLAATFVRVNLALL
                     IGAAWTIPAGVAIGFNPRMARIAQPLAQIAASVPATA"
BASE COUNT         1118 a         1635 c         1784 g         1156 t
ORIGIN      
        1 ttacgggagt cacaaagcga gctacgccaa ctcggtcccg gaaaaacgca aggtcaaata
       61 cgcaatcgtt gacaccagca aagtcgaggc cggcgtccaa ctgacgcagg atgatttgcg
      121 ggagtactac gatcagcacc gcaacgagta ccgcgttccg gaccaggtga aggtgagtca
      181 catctggatc aagatgccct tgcccggcga cgacggcaaa gttgacgaga aaaaggttgc
      241 tgaagcccag cagcgcgctg acgacctgtc gcggcagctt aagaacggcg gcaagttcga
      301 cgaactggcg aagaaatact ccgaagattc cggcagcgcc aacgtcggcg gctcgttagg
      361 atggatcggc cgtggtcaga tgactcccga gtttgaaaaa gcggcatatt cgctctccaa
      421 aggccagatc agcgacgtag tgaagagcac cgacggcttc catatcattc gcctggatga
      481 ccggcaggaa gctcatgtca agacgctggc tgaagtgaaa gatcagatcg aacccatcgt
      541 caagcatcaa agagcgcagc agatcgcgca aaggcaggcc gattcactgc tgcgacaggc
      601 acagtccaaa tcggtgggac tggatgcggc tgctggcgcc atgagcattc cagtgatcac
      661 gtccgacttt ttctctcgca aagacatgct gccggggctg gggccggctc cgcagttcat
      721 ggatgcagtg tttacgctgg cagagaattc gcctccggag atggcctcag cgtcgcaagg
      781 aatcgtcgtg ttccagttgc tggcgatcaa accggcggcg acaccggcct ttgaagaaat
      841 tcgcagccgc gtcgaagatg aattcaagag cgagcggtct tcggtgttgc tgcagcagaa
      901 ggttcaggaa ttgtcggacc gcgccaaggc tgagcatgat ctgaaacgcg ccgccaagga
      961 attgggcgcg accatcaaga ccagcgactt cgtgctgcct gacggccagg tgcccgatgt
     1021 gggctcgatg acggggcagg cttcggttgc ctttacaatg aagccgggcg agatcagcgg
     1081 cccgatcacc agcggagcca atgccgtggt gatttctgtg ctcgaggatc agaagcccaa
     1141 tgaggccgat tacgccgcca agcgtgacca gatccgcgat acgctgctgc agaacaagca
     1201 gcaggagctc ttcggtcttt tcgtttccaa cctgcgcgat cagatggaga agtcaggcaa
     1261 gatcaagatc aaccaggaag agatgaagaa cttgacccgc acgggaagcg agcagggaat
     1321 gtaacggcgg caccaatgtc ctttccgcgc gccgctggaa ttctgttgca tcccacctca
     1381 ttgccgtccc gaggcggcat cggcgacttc ggcccagccg cctaccagtt cgtggaattt
     1441 ctcgcttcgg ctcgtcaggg gctttggcag gtcttacccc tggggccggt cggctatggg
     1501 aattcgcctt attcctcgac ctcggcgttt gccggcaacc cgctcttgat cagcctggag
     1561 cgtctggctg agcggggatg gattgatcgc tcaaagcttg actcggcaaa tcctgactcg
     1621 gcaaatcctg actctcagat ggcggacacc tccgccggca gtcgcccggt cgaatatgac
     1681 caggtctttg ctcagaagat gcccgttctg tttgaggcgg cacggaacct tttgcgagcc
     1741 ggctcaagca gcgcgctgga ccgtctgaag acatttcaga cggagaatgc gtggtggctg
     1801 gacgattttg ttcttttcga tgcgctccgc gcacggcata agctgggcag ctggaatcaa
     1861 tggccgcacg atcttgcata tcgcgaccca tctgctctag cgaaggctcg cgcggagctg
     1921 gcagccgata cacaaatccg cagcgctctg caatttgcct tctacgaaca gtggcgagcg
     1981 ctgcgccgct attgcgcgga gcgtgccatt cgcattgtcg gcgatgtcgc tatttttgtg
     2041 aactatgaca gcgccgatgt atggagccac cgcgaactgt tccggctgaa cgaggatctg
     2101 cagcccgagg tagtgtcggg cgtccctcct gactttttca gcaagaccgg acagcgctgg
     2161 ggaaatccgc tttaccgctg ggacgtgatg aaagagcgcg gatatgcctg gtggattcag
     2221 cgcctgcgct gggcgacgca gcattgcgac tacattcgcc tggatcactt ccgcggattc
     2281 gaccagttct gggagatccc tgcaggcgaa gccacagcgg tgaatggccg ctgggtggac
     2341 gggccgcgcg acgacctgtt tgcgaagctt catgaagcgc tcggcggcct gccgttcttc
     2401 gccgaggatc tcggctacat tacgcccgag gtgcacgcac tgcgagacag gctgcaaatc
     2461 cccggcatgg cggtcctgca gttcggcttt ggtgacgagg gagcgcatat ctatttgccg
     2521 catcgcgcag cggggaaagt gatctatacg gggacccacg acaacgacac gatagtcggg
     2581 tggtggcatt ctggggccag cgagcacgag cgccgcaatg cggaggccta tcttgggcgc
     2641 tcggacgatg gcgtgcactg ggcgtttatc cgcgccgcgc agtgttcgcc cgccagcatg
     2701 agcgtcatcc ccctgcagga tgtcctgggc ctgggaagcg aagcccgcat gaacacgccc
     2761 agcgtctatg ggggcaactg gcgttggcgt ctcgacgcca ctcaactgcg cggcgaactg
     2821 gcggagaagc tggcccgtct cgcggaaatg acggaccgcc tgcctcaggc gattgcggtg
     2881 ccagcggatg atggcttcgt tgcttgagcc gaacccctgt tgaaattgtg aatccagtgt
     2941 taaagctgtg ttttgcgctt tgccgcagat cgtgtatagg aaatacggga gctaaggcgt
     3001 gaacgaggcg atcatcgaag cggtggcgat cgagaaatcc tacccgcagc ctgacggcac
     3061 gcgcatccag gtgatcagcc caaccaacct ggtcatcgag ccggggaaga tcatcgcgct
     3121 gctggggccg tcgggttgcg gcaagtcgac gcttctgcgt atgctgaccg ggctttcgca
     3181 gccatcttcc gggatgctgg cctggcacgg caagccgctc aacggtcaga tgcccaacgt
     3241 tgccatcgtc ttccagagct ttgccctctt tccctggctc acggtcctcg aaaatgtgga
     3301 agcgccgctc gaggcgcgcg gcgtagcggc ggtggagcgg cacaaacgcg ccctgcgcac
     3361 gctcgacacc gtcggactcg acggtttcga gaccgcctat ccgaaagagc tctccggcgg
     3421 catgaagcag cgcgtcggtt tcgcacgggc cttggtcgtc gagccggaag tactcttcat
     3481 ggacgagcca ttctcagcac tcgacgtcct taccgccgag aacctgcgca gcgaactgct
     3541 cgaactttgg ctcaacaaga agatgccgac cagcgcgatt ttcattgtga cgcacaacat
     3601 tgaagaagcg gtcatgctgg cggaccgcgt catggtgctg ggccggaacc cggcccgcat
     3661 acgctccgac ttcaatgtgc ggctcaaaca tccgcgggac cgcaagtctc cgcgttttgt
     3721 cgagttagtc gactacatct acaaagtgat gaccgagccc gaagtcgagc acgcgttgcc
     3781 ggatgcagag accaccgcgg agatcatcct ccccaaggcg gggctgaaga agcaggaagc
     3841 gccagttcgc actgcgcggt accagatgct gccccatgca cgtgtgggtg gaattgcggg
     3901 ccttttggaa ttgcttcacg atcggggtgg gcgcgaggat ctgtttcgcc tttccgaaga
     3961 actggtgatg gacgtggagg atctgctgcc cattcttgag gcgtgtgtgc tgctcggctt
     4021 tgccttgctg aaagagggcg atgtgcagat tacgccgcaa ggcatcagat ttgccgacgc
     4081 agatattcag cagcgcaaag tgttgttccg gcaagcggcc ctggagcacg tgaccatcct
     4141 gaaacagatt gacagcattc tgagacgcaa atccgaccac tcgattgcag atgaattctt
     4201 tcacgacatt ctcgatgagc attttgccga ggatgaagtg cagcgccagt tcgaaactgc
     4261 gatgaattgg ggccgctacg ctgagatctt tgactatgac cgggaaaacg gccggctggt
     4321 gctgaccgaa cctgcggctt cggatgcggt gccggttagc cagaatgggc cttctgctcc
     4381 cgaatcctga gccgcctggc cgaatcatga cccacaccag aaaaaccggc ggcttgcgcg
     4441 gattctccgc ataccagctc ttgacccagc tgccgagagt ggagatttct tatctcccgg
     4501 acctgctgat gttcggggtg ggcattgccg tgttctacgg cgtgctcgtg gtggggcgga
     4561 cgtggctcgg cccgttcacg ccagaggtcg aaatctcgcg cagcccgctg gctctgcccg
     4621 cctacgcggg gtattcactg ctgcgcatca ccatcgccta tgttctgagc ctcgccttca
     4681 cgctggtgta tggatacatc gcggcctaca atccgcgagc agaacgcttc atgattccgc
     4741 tgcttgacgt gctgcagtcg attcccgttc ttagttttct tccgggcgtg atgctggcca
     4801 tggtggcgct gtttcccggc aggcaactgg gagtggaggc aggcgcgatt cttctgattt
     4861 tcacaggtca ggtctggaat atggccttca gcttttatgc ctcgctcaag agcattccgt
     4921 ccgagatgcg cgaggcagcc aaggtatacg gcttcagctg gtggcagcgc ttcgtggaga
     4981 tggagctgcc gttctccgcc atcggcctgg tgtggaattc catgatgtct gtcgcgggcg
     5041 gatggttctt cctgatggcg tgcgagatgt ttgttctggg ctcgcgcgac ttccgcctgc
     5101 ccgggctggg atcgtatctg caaaccgcgg cgagcgccgg cgatacgcca tcgattctat
     5161 ggggcgtcgg caccatgatc gccgtgattg tgctgctgga ccagttcttc tggcgtcccg
     5221 tgattgcatg ggcggagaag tttaaggtgg aacaggtcga gagtactgag gctccgcgct
     5281 cctgggtgct cgacctgatt catcattcgc gcagcctggc gcggattcgc cagaacacct
     5341 tcagcccgct gggcgaacgc ctgatgcttc atttctctca caaagactcc agtggtaagg
     5401 ctggtctgca aagcgcgtgg aaaacctggc tggcgcgctt gctggggctc gccctgctgg
     5461 ccggaatggg gtacggagtc gttcgcgtgg cgatcattct gagtggcctg cagaaggcgg
     5521 agttgcagga agcaggaatc ggcctggcgg ccacgtttgt gcgcgtcaat ctcgccttgt
     5581 tgatcggcgc cgcttggaca attcccgccg gggtcgcgat cggattcaac ccacgcatgg
     5641 cacgtatcgc gcagccgctg gcgcagatcg cggcctcagt gccggcgacg gcg
//
LOCUS       DASPUL010000258         3920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1273515, whole genome shotgun sequence.
ACCESSION   DASPUL010000258 DASPUL010000000
VERSION     DASPUL010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273515"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1761
                     /locus_tag="VEJ00_06425"
     CDS             <1..1761
                     /locus_tag="VEJ00_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00830.1"
                     /translation="PTIHRRTMIRNKQRSRGRESALSIALAVVAAVWAGPALPRLQAQ
                     APAQHKILLQADLRPGTVLRYELEAAGSFLPVNGANGAILMPPRGPCDYAMAAIVTLR
                     PQAADKDGNTPVEARYSEARMTSVRCALFSDADFQKRLAVLQSSPVMFRVGPHGETAL
                     RHSSGGYFKYWDGAELLRKVTQDLLQTQFAPQPVAEGDSWKPRGQFAYSHDRALKDLE
                     LSGADLRFRKMVEVDGKSCAWVTSQYVFSPVDLPAMGIAYGGRIYPGVGNNAVAAVLH
                     ISLLIDPASHHVAWLHRSQTIDNKLTLASPYDDSDPAEEPSDDTSDDPENPTPDMSSM
                     RSDNPERYPFMTFHFQEEARARLLPDERSAEWLAALKKFEATPEPESGMAPVSVTKTL
                     LPNPIIQAAKPAVVKRSTRVVVDSDSVMATPAGFTRYEKGLCRDQWFCATVSVALPGE
                     VQISEDTPLRTVYLARKGDLLVNVAVGPALDRKPAGLTEDEELRKQAEYYLANYVWLA
                     VKPGIGTSFASATLDGYPGLITDFNATQRDLANIHGMLGLMLTPWGKVVPVSCSSDHG
                     ASEDLRAVCEKVITTVSLRR"
     gene            complement(1983..3287)
                     /locus_tag="VEJ00_06430"
     CDS             complement(1983..3287)
                     /locus_tag="VEJ00_06430"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HXX00831.1"
                     /translation="MSKQALSRFSLVLLTAGLCALAALPALADSQARIVRLSDVQGSV
                     QIDKNSGAGFENAFVNLPITQGTQLQTRDRGRAEVEFEDGSTLRLAPNSTLEFITLGL
                     NDAGKRISAIHLVEGMAYVNWLGKSGDEFTLSFSLEKISLDHAAHFRVDTSAQAANLA
                     VFKGDIEVEGPAGKVAVEKKKTATFDVANNDKYALASNIEEEPLDSWDKEAIAYHDQY
                     AKNNNTPYGYGYSDLNYYGAYTNVPGYGMMWQPYFTGIGWDPFMDGAWAWYPGFGYMF
                     VSAYPWGWTPYMYGNWMFVPGFGWMWQPGYWGSFVGVPRVVGRPPVNFHPPIVPTAGS
                     VKTVVVGRGGAVSTLSSSRFTVRAGSAGIGIPRGSISNLGHLNREVAKTGFVAVHSQP
                     QFSASSGRMGGFGGGRSYGAAPVSHSAGSISTGHVSSGGVHR"
     gene            complement(3452..>3920)
                     /locus_tag="VEJ00_06435"
     CDS             complement(3452..>3920)
                     /locus_tag="VEJ00_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00832.1"
                     /translation="ETYQTDFTFSDATRARVFALAAQAHYFSGKIDSGNRKLAFTGTK
                     KLSYTDAQRNVSATYNFSPQQPVQQLTALFQAVAATMEFARRLTYDHRYQKLALDDEL
                     KRMEAEAHSGELAELDAARPILQEIFDDSSVINVVRARAQRLMEMAKSVPTAR"
BASE COUNT          748 a         1129 c         1278 g          765 t
ORIGIN      
        1 ccgacgatcc accggaggac gatgatccgg aataaacagc gatcccgagg aagagagtct
       61 gcgctctcca tagccctggc agtcgtcgcg gccgtgtggg cgggcccggc cctgccacgg
      121 ttgcaggcgc aggcgccggc acagcacaag attctgttgc aggcggactt gaggccgggg
      181 actgtgctgc gttacgaact ggaagcggcg gggtctttcc ttcctgtcaa tggcgccaac
      241 ggagccatcc tcatgccgcc tcggggacct tgtgactacg cgatggctgc cattgtcacc
      301 ttgcgtccgc aggcagcgga caaggacggc aacactcctg tcgaagcgcg gtatagcgaa
      361 gcacggatga cgagcgtgcg ctgcgcgctc ttctccgatg cggatttcca gaagcgtctc
      421 gcggtcctgc aatcgtcgcc ggtgatgttc cgggtggggc cacacggcga gacggcgcta
      481 cgccacagtt ccggggggta cttcaagtat tgggacggag ctgaactgtt gcggaaggtc
      541 acgcaggatc tgctgcagac ccagttcgcg ccgcagccgg tggcagaagg cgattcgtgg
      601 aagccgcgcg gacagttcgc gtattcgcat gaccgtgccc tgaaagatct cgaactcagc
      661 ggagcggatc tgcgcttccg caagatggtc gaggtcgatg gaaagtcctg cgcgtgggtg
      721 acgtcgcaat acgtcttctc gccagtcgat cttccggcga tgggaatagc atacggggga
      781 cggatttacc cgggggtcgg caataacgca gtcgccgccg tgctgcacat ttcgttgctc
      841 atcgatccgg cgagccatca tgtggcgtgg ctgcaccgct cgcagaccat cgacaacaag
      901 ctgacgctgg catcgcctta cgacgacagc gatcctgccg aggagccctc ggacgacact
      961 tccgacgatc ccgaaaatcc cactcctgat atgtcgagca tgcgctccga caatccggag
     1021 cgctatccgt tcatgacgtt tcacttccag gaagaagcgc gggcgcggct tttgccggac
     1081 gagcgttcgg ccgaatggct ggctgcattg aagaagtttg aggcgactcc ggagccggag
     1141 agcgggatgg cgccggtttc ggtgacgaag acgcttctgc ccaacccgat catccaagcg
     1201 gcgaagccgg cggtggtgaa acggtcgact cgggtggtcg ttgattccga ctcggtgatg
     1261 gcgacgcctg ccgggttcac tcgctacgag aaaggcctgt gccgcgacca atggttttgc
     1321 gcgacggtat cggtggcgct gccgggagag gttcagattt ctgaagacac gccgctgcgc
     1381 accgtgtatc tcgctcgcaa gggggacctt ctggtgaatg tagcggtcgg tccggcactg
     1441 gatcgaaaac ccgcggggct gaccgaagac gaggagctgc gcaagcaggc ggagtactat
     1501 ctggcgaact acgtctggct ggcagtgaag ccgggaatcg gcacgagctt cgccagcgcc
     1561 acgctggatg gatatcccgg gctgattacg gatttcaacg ccacccaacg tgacctggcc
     1621 aacattcacg gaatgctggg cctgatgttg acgccctggg gcaaggtcgt gccggtaagc
     1681 tgcagctcgg atcacggggc gtcggaggac ttgcgggcgg tgtgcgagaa ggtgattacc
     1741 acagtgagtt tgagaaggta actcctgcgt tgtcatcctg agcggagcag gacggtgagc
     1801 gaagcggatc gtcctgcgca gtcggaggat ccctacctgc tcatcgagtc ttctggtccg
     1861 tcgatcacat cacagttatc ttgcgggcag ggatccttcg actccgcgtg ggcttcacgt
     1921 tcgtgaagcc cacgctacgc tcaggatgac attttccgca cgacactttc atgaacaact
     1981 ggctagcggt gcacgccgcc gctcgaaacg tggcccgttg atatgctgcc cgcgctgtga
     2041 ctgaccggag cagcgccgta gctgcgtcct ccgccaaaac cgcccatcct gccggagctc
     2101 gctgagaact gcggctggga gtgcacagcg acgaatcccg tcttggcgac ctcacggttc
     2161 agatgaccga gattggaaat ggagccgcgg ggaatgccga ttccggcaga gccggcgcgg
     2221 accgtgaagc gcgacgagga cagcgtcgaa accgccccgc cacggcccac aacgaccgtc
     2281 ttcacgctac ccgcagttgg cacaatcgga ggatggaaat tcacgggagg tctgcccacg
     2341 acgcgcggca cgcccacaaa cgagccccaa tagcccggct gccacatcca gccgaagccg
     2401 gggacgaaca tccagttgcc gtacatgtac ggcgtccatc cccaggggta tgcggacacg
     2461 aacatgtagc cgaagccggg ataccacgcc caggcgccgt ccatgaacgg atcccagccg
     2521 atgccggtga aatacggctg ccacatcatg ccgtagccgg gaacgttcgt gtatgcgccg
     2581 taatagttca ggtcgctata gccgtatccg tagggggtgt tgttattttt cgcgtactga
     2641 tcgtggtaag cgatggcttc cttgtcccag gaatcgaggg gttcttcctc gatgttgctg
     2701 gctagcgcgt acttatcgtt gtttgcaaca tcgaaggtgg ccgtcttctt cttctcgacg
     2761 gcgaccttgc ctgctggtcc ttcgacttcg atgtcgcctt tgaagactgc cagattcgcg
     2821 gcctgggcgg aggtgtcgac gcggaagtgc gccgcgtggt cgagcgagat cttctcgagc
     2881 gagaaactca gggtgaattc gtcaccgctc ttgcccagcc aattgacgta ggccatgccc
     2941 tcgacgagat gaatcgccga aatccgcttc cccgcgtcgt tcagtccgag ggtgatgaat
     3001 tcgagggtcg agttcggggc caggcgcaag gtgctgccgt cttcgaattc gacctcggcg
     3061 cggcccctgt cgcgggtctg caactgtgtc ccctgcgtga tggggaggtt aacgaaggcg
     3121 ttctcgaatc ccgcgcccga attcttgtcg atctgaaccg atccctgcac gtcgctcagg
     3181 cgaacgatac gagcctgcga atcggccagc gcgggcaggg ccgccagcgc acacaatcct
     3241 gctgtcagaa gcaccaaact gaatctggac agagcttgtt tggacatagc aaccccctgg
     3301 agggtcttcc ctgcatatta gaacacggtt cggttgcccg agttgtcaac cgttggtgca
     3361 gtgttcggaa gattacggtt cagcagatgc atggccgggc gatcaagggc aatgggattc
     3421 agcggtcgga atctccagat ttctgaaccg cttagcgcgc tgtgggaaca gacttggcca
     3481 tttccatgag ccgctgcgcg cgggcgcgga cgacgttgat caccgagctg tcgtcaaaga
     3541 tctcctgcag aataggccgg gcggcgtcga gttcggcgag ttcgccggag tgggcttcgg
     3601 cttccattcg cttgagttcg tcgtcgaggg cgagcttctg gtagcggtgg tcgtaggtca
     3661 ggcgacgggc aaattccatg gtggcggcca cggcttgaaa caacgccgtc agctgttgga
     3721 ccggttgctg cggggagaaa ttgtaggtgg ccgaaacatt ccgctgcgcg tcggtgtatg
     3781 acagtttctt ggtgcctgtg aaagccagct tgcggttacc ggagtcgatc ttgccggaaa
     3841 agtagtgagc ctgggcagcg agggcgaaga cgcgggcgcg agttgcgtcg gagaaagtga
     3901 agtcggtctg gtaggtctcg
//
LOCUS       DASPUL010000259         3471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_593255, whole genome shotgun sequence.
ACCESSION   DASPUL010000259 DASPUL010000000
VERSION     DASPUL010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593255"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VEJ00_06440"
     CDS             <1..467
                     /locus_tag="VEJ00_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00833.1"
                     /translation="QFAHIFAPRPEVALKEMLRVARPGGTIAFSTWPPESLVGSTQKV
                     RARYMPPPPVAMPSPDLWGDPGIVRERFGSAVMDIVFDRGTILAPALSRQHYRLTVER
                     TAGPIIKVIQHLEASDPARLQAFREEFDAAVAPYVCDNVVRQDYLLTRARKH"
     gene            complement(546..1523)
                     /locus_tag="VEJ00_06445"
     CDS             complement(546..1523)
                     /locus_tag="VEJ00_06445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXX00834.1"
                     /translation="MPKYSIVVPLHNEQENVTDLYDRLKAVMEAHGESFEIVLVDDGS
                     NDHTFPMLREIAAVDSRVTVVKLRRNFGQTAGLAAGFDHARGEYIIAMDGDLQHDPGD
                     IPLFLEKIAAGYDIVSGWRKNRVDNFWLRRIPSRVANWMMAKISGVDIHDFGTTFKAY
                     RRDILEQVPLYGELHRFIPALASWHGATICEVPIRNVNRERGASHYGITRTFRVFFDL
                     LTIRFLLRYLSRPLHFFGGFGMLSILTGSGVVLWLGVEKFLRNVPVMATHGPLMMASL
                     GLLLGGLNLLAIGLLGEMQVRHYHEPSRRAPYSVDRVLRSQNEEHSVSE"
     gene            complement(1713..2798)
                     /locus_tag="VEJ00_06450"
     CDS             complement(1713..2798)
                     /locus_tag="VEJ00_06450"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXX00835.1"
                     /translation="MFDKNRFAPRARAEAFADRLGLRLPILLAPMAGACPPSLSIAVA
                     NAGGMGACGALLMQPAEIEAWAAEFRQGSRGHFQINLWIPDPPPVRNAELEKKQREFL
                     AQWGPPVPPEAGDSVPPDFAMQCQAVAAARPKAISSIMGLYPPHFVAGMKESGTLWFA
                     NATTVAEGKAAEQAGADAIIAQGMEAGGHRGSFHAEEAERQMVGLMSLLPQIVDAVNV
                     PVIAAGGIADARAIAAALILGASAVAIGSGFLRSPEARMHPAYADRLARTEAHETMIT
                     RAFSGRAGRSAATAYVRAAAAEDAPGPAPYPVQRGLTRAMREAAQKAGDADRMQLWAG
                     QSAKLARNEPAGEIVQQLWRDALRLLP"
     gene            complement(2950..>3471)
                     /locus_tag="VEJ00_06455"
     CDS             complement(2950..>3471)
                     /locus_tag="VEJ00_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00836.1"
                     /translation="VVFSSGNESFGKAVIPLAIDPYDGKQFSLSGVAMSNDIRPASDM
                     ATGLDAALLEDRKPLVVRGMHFIPSASDHFKTTDNVAIYLELYAPVLLGPHPPTIGVE
                     MRVTDRKTGQQKVDTGAKDLTGAIQAGNPVIPLGLRVPTNSLTPGSYRIELRGFDSAG
                     ALTAFHSADFELE"
BASE COUNT          646 a         1053 c         1144 g          628 t
ORIGIN      
        1 gccagttcgc gcacattttt gcgccaagac cggaagtcgc attgaaggag atgctgcgcg
       61 tggcgcggcc aggaggaacc atcgcttttt cgacgtggcc tccggaatcg ctggtaggaa
      121 gtacgcagaa agtgagggcc aggtacatgc cgcctccgcc ggtcgcgatg ccctcgccgg
      181 acctctgggg cgatcccggg attgtgcgag agcggttcgg gagcgcggtc atggatatcg
      241 tgttcgatcg cggaacgatt cttgctcccg cgttaagccg gcagcattat cggctgacgg
      301 ttgagcgcac agcgggaccg atcatcaaag tgattcaaca tttggaggcg agcgatccgg
      361 cgcgcttgca ggcttttcgc gaggagtttg atgcagcggt cgcaccgtat gtgtgcgata
      421 acgtggtccg ccaggactac ttgctcaccc gggcaagaaa acactgacca ttgcgcggat
      481 tatcgcgaag aaagcagatt cgtcgctttg ctcggaatga caaggtttgg ggagtgttcg
      541 ggaacctact ccgagacgct gtgctcttcg ttttgtgagc gcaggacgcg gtcgaccgag
      601 tacggagcgc ggcggctggg ttcgtggtag tggcggacct gcatttctcc cagcaggccg
      661 atggcgagca ggttcaagcc gccgagcagc aggccgagag acgccatcat cagcgggccg
      721 tgcgtggcca tcaccgggac gttgcgcagg aacttttcca ctccgagcca gagcacgacg
      781 ccgctgccgg tgagaatgct cagcatcccg aagccgccaa agaaatgcag cggccgcgac
      841 aagtagcgca gcaggaagcg gatggtcagc agatcgaaaa acacgcggaa ggtgcgcgtg
      901 atgccgtagt gcgaagctcc gcgctcgcgg ttcacgttgc ggatcggcac ctcgcagatg
      961 gtcgcgccgt gccatgaagc cagcgccggg atgaagcggt gcagttcgcc gtagagcgga
     1021 acctgctcca gaatgtcgcg gcggtacgct ttgaacgtgg tgccgaagtc gtgaatgtcg
     1081 acgcccgaga tcttcgccat catccagttg gcaacacgcg aaggaatacg gcgcagccag
     1141 aagttgtcga cgcgattctt gcgccagccg ctcacgatgt cgtagccggc ggcgattttt
     1201 tcgagaaaca gcggaatgtc gccgggatcg tgctgcaggt cgccatccat ggcgatgatg
     1261 tattccccgc gcgcatgatc gaagccggcg gcgagtcccg cggtctgccc gaagttgcgg
     1321 cgcagcttga ccacggtcac gcggctgtcg acggccgcga tctcccgcag catgggaaaa
     1381 gtgtggtcgt tcgatccgtc gtccacgaga acgatttcga aactctcgcc gtgcgcttcc
     1441 atcaccgctt tcaggcggtc atagaggtcg gtgacgttct cctgctcgtt gtgcaggggg
     1501 acgacgatgg agtacttggg cacagttttt agattatagc ccgaatctgg acgggagtgc
     1561 gttgattgtg agggaactgc agtaacgcag cactcggttg aaacccgccg cgcagtcatc
     1621 ccgaaggccc gcgctttcac gagcgggcag gcgcatgaga tccctgcctc cgccagaaaa
     1681 ccggctacgc tcgcgatgac gccacccaag ggctatggca gcagccggag ggcgtcgcgc
     1741 cacagctgct ggacgatctc gcctgccggc tcgttgcgcg ccagtttggc cgattgaccg
     1801 gcccacagct gcatgcggtc ggcgtcgccg gctttctgcg ccgcttcgcg catggcgcgg
     1861 gtcaggccgc gctgcaccgg ataaggagcc ggaccggggg cgtcctcagc cgctgcggcg
     1921 cgcacgtagg cggtggcggc gctgcgcccg gcgcgtccgc tgaaagcgcg cgtgatcatg
     1981 gtctcgtgcg cctcagtgcg ggcgagccgg tcggcatagg caggatgcat cctggcctcg
     2041 ggtgagcgca ggaaaccgga tccgatcgcc accgcacttg cacccaggat gagcgccgcc
     2101 gcaatggcgc gcgcgtcggc gatgcccccg gcggcgatca cgggaacatt aaccgcatcg
     2161 acaatctggg gcaggagcga catcaggcct accatttggc gctcggcctc ctccgcatga
     2221 aaagagccgc gatgtccgcc ggcctccatg ccctgcgcga tgatggcgtc ggcgccagcc
     2281 tgttcggcgg cttttccctc ggccaccgtg gtggcgttgg cgaaccacag ggttccgctt
     2341 tctttcatcc ctgcgacaaa atgcggcgga tacagaccca tgatcgacga gatcgccttc
     2401 ggcctcgcgg ccgccacagc ctggcattgc atggcgaaat caggcgggac ggaatcaccg
     2461 gcttcaggag gcaccggagg tccccactgt gccaggaact cgcgctgctt tttttcgagc
     2521 tctgcgtttc gcaccggcgg cggatcaggg atccagagat tgatttgaaa atgcccccgg
     2581 ctaccctggc gaaattcggc ggcccatgct tcgatctccg cgggttgcat cagcagagcg
     2641 ccgcatgccc ccatgccgcc ggcgttggcg acggcgatcg agagcgaggg cggacaagct
     2701 ccggccatgg gcgctagcag aatgggcaag cgcaggccta ggcgatcggc aaaggcctcg
     2761 gcgcgggcgc ggggggcgaa gcggtttttg tcgaacatgt tcaagtttat ttcagagtta
     2821 tttcgtgcag aagtaaccgg aggccgcgcg cgctcgtccc ttgcgcgcac gcagcgccct
     2881 tgctctggcg ctccatgcga attggatggt cccgggagag caccggaacc acgaaaaaaa
     2941 cgcgcccgct tattcgagct cgaagtcggc cgagtgaaaa gcggtcaacg cgcctgcaga
     3001 atcgaatccc cgcagctcaa ttcgatagga acctggcgtc agcgaatttg tcggcacgcg
     3061 caaccccagc ggaataacgg gattccccgc ctggattgca cccgtcaggt ccttcgctcc
     3121 ggtatccacc ttttgctggc cggtcttgcg atcggtgacg cgcatctcca cgccgatcgt
     3181 cggaggatgg ggaccgagca acaccggggc atacagttcc agataaatgg ccacgttgtc
     3241 cgtggtcttg aagtggtcag aagccgaggg aatgaagtgc atgccgcgca cgaccagtgg
     3301 cttgcggtct tccagcaggg ccgcatccag gccggtggcc atgtccgacg cggggcgaat
     3361 gtcgttgctc atcgccacgc cgctcaggct gaattgcttt ccgtcatagg gatcgatggc
     3421 cagcgggata acggccttgc cgaagctctc gttgcccgaa ctgaacacca c
//
LOCUS       DASPUL010000260        11567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1690306, whole genome shotgun sequence.
ACCESSION   DASPUL010000260 DASPUL010000000
VERSION     DASPUL010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 11567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11567)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11567
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690306"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1121)
                     /locus_tag="VEJ00_06460"
     CDS             complement(<1..1121)
                     /locus_tag="VEJ00_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HXX00837.1"
                     /translation="MTISSCLLEAYLKCPTKCWLRSAGEQITDGTCVQCTQARNESYV
                     AAEIRRLLSRTLQSECMLSPSADHLKACKWRLSTGVLAQTPNLESYIHAVECLPSARR
                     GKPAHIIVTRFISTNKISKDAKLLLAFDALALSETLKQEVGLGKIIHGDNHATLNVCT
                     SALELEVRQHIAKIDALLSSPSPPDLVLIPHCADCEFQSRCRQKALENDDLSLLSGMT
                     EKERKKLHDKGIFTVTQLSYTFRPRRRPKQVRDKREKYHHSLRALAIRQNKIHIVGNP
                     KIVIEGTPVYLDVEGLPDRDFYYLVGVRVGDRNSAVQYSLWADSVEDERKIWNEFIGI
                     LTTITDPVLIHYGSYETIFLKRMGDRYGVPPAQSRVAGAV"
     gene            complement(3078..4202)
                     /locus_tag="VEJ00_06465"
     CDS             complement(3078..4202)
                     /locus_tag="VEJ00_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXX00838.1"
                     /translation="MQLKPFLLDAWLDQYEHDIEFNLAASTGPTWTVNDILTLADDET
                     HYRFLNHKLVYSRPAGADSLREAIAEMQRVPVDAVQIVTGASEALVALMWLAAEPGAN
                     VIIPLPGFTTFSALPESLGLETRFYRVRRENGFRIDPDEIKRLADSKTKLILLNSPHN
                     PTGTTIDDEEMEALHDFTAERGIQLVSDEVYHPIYYGKQTKSAARLPHATVIADLSKA
                     FSIAGVRTGWMIEHDAQRRQQYWTARAYFSICNSTTGEILSEIAIRKRDVVLGKTQEA
                     ATRNLKLLEHFMADHRDVLGWIPPQGGMTAFPWLLSGENDRPFCQAATERGILLAPGD
                     CFDTPSHFRLGFAAAGDNFSQALDRFGAFVKSWSAKMVTA"
     gene            4547..6148
                     /locus_tag="VEJ00_06470"
     CDS             4547..6148
                     /locus_tag="VEJ00_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HXX00839.1"
                     /translation="MTWASPILLPDEFNAAAFFVDRHIGEGRAGKTAIICGDVHVTYG
                     QLSVLVNQFGNGLKRLGIRMEERVFLLLLDTPEFAVSFFGAIKIGAVPVPANTLLKPS
                     DYNYLLNNSRARVAIVNNSLLPLLESIPKSALRYLETVVVVGGAAVSGTISFGDLIRN
                     CSCDLDPAPTGKDDAAFWLYSSGSTGSPKACVHLQHDMVVSAERYAKAILKITEEDRF
                     FSVAKLFFAYGLGNGLYFPLTVGGTSILLPGPPRPQSVFEAIERDKPTLFFSVPSNYV
                     KLLAHPAPAEREFDLSSIRHAISAGEALPASVFCRFKERFGVEILDAIGSTEALHMLI
                     SNVPGAIRPGSSGKVIPGFEAKLVDDNDQPVAPGEIGNLMVKADSTCAYYWNEHEKTK
                     NTFTGQWLRTGDKYYQDADGYFWYAGRSDDMLKVSGVWVSPIEIERVLSEHPAVLEVA
                     VVFRQDADELSKPAACIVLRDGASASLQMACELQEFVTDRLPIYKRPRWVEFMRELPK
                     TATGKLQRYKLREIATAGASGTVEE"
     gene            complement(6279..7520)
                     /locus_tag="VEJ00_06475"
     CDS             complement(6279..7520)
                     /locus_tag="VEJ00_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HXX00840.1"
                     /translation="MGIPEENSLVYDWTEQGPRRARWPGHVMLDDETLRDGLQSPSVT
                     DPPIDTKLQILHLMESLGIETADVGLPGAGERQREAVLQLCREIAQHHMRIRANCAAR
                     TLMVDIRPIAEATQLTGVPIEACLFIGSSPIRQYAEEWELDDILRHSREAITFALREG
                     LEVMYVTEDTVRSSPDQLRILLKAAVEAGAKRVCLCDTCGAAAPDAVYNLVTWVGALL
                     CEIGSPQVGIDWHGHRDRGLDLANTMVAIEAGASRVHGTALGIGERVGNTPIEQILVN
                     LKLLGIRNDDLTRLPEYVQLVSQVTRVPIPVNAPIVGHDAFRTATGVHAAAVIKAHKK
                     GHTWLADRIYSGVPAGWIGRSQKIEIGPMSGNSNVQHYLASHGVPVTPELTKRIMDEA
                     KKSPRVLTDGEILEIVRGFSP"
     gene            complement(7536..8267)
                     /locus_tag="VEJ00_06480"
     CDS             complement(7536..8267)
                     /locus_tag="VEJ00_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX00841.1"
                     /translation="MTNFNSLRCTRCGRVAAEPDKFCAECGLFLRDAAVDQRLLLAMV
                     HEREGRSQEARRELERLLTTEPDHVLANHLLGTLHFHDGLLDQAARYYEKAVAAAPSF
                     TLGYYDLGVAYYHRGDMPQAMRAFRRCLEIDPNYNAAHYRLALALFHNGHLDEAREHF
                     ELSLTLTPEYLMAHYHLGIIHERQGEYEAAAREFEHSLEESSGDVSSLYHLALVRAAQ
                     GDHDSAERLRQRARSFAKSQPVGSK"
     gene            complement(8284..9012)
                     /locus_tag="VEJ00_06485"
     CDS             complement(8284..9012)
                     /locus_tag="VEJ00_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXX00842.1"
                     /translation="MNQASDLNGRIAVVTGGSGVIGQAIARALQAAGAYVSLLDVATP
                     KGLALPWIECDVRDDSSVAHAVSEVTSKQGGVDIAVHAAGVSRDAVVWKLSLDDWDFV
                     QSVNLRGAFLLIRHSLPRMRVRRGGRIVLIGSINGSRGKFGTSAYSASKAGLLGLAKT
                     VAREAGRFGVLINVIEPGWVRTPLTDSVPQPIRDAALAESLVGTFVEPEDIGHAVTFL
                     CGPGGGHITGQVLRVDGGQFLGPT"
     gene            complement(9014..9799)
                     /locus_tag="VEJ00_06490"
     CDS             complement(9014..9799)
                     /locus_tag="VEJ00_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXX00843.1"
                     /translation="MSFEKIRLKFTHQERVARITLASPKANILDRAMIRELEEAVSQC
                     ARRPLNALVLTAEGPHFSFGASVQEHLPDQIAGTLRALHALLRRLHEAPAPVIAAVRG
                     QCLGGGFELALACDLIVADNTAQFAVPEIKLAVFAPAASALLPLRVGQALAARLLLTG
                     AAITADEAARSGLVTRVAEDLDAEIERWLESDFLPRSPSSLQFGCRAARMSLRRALGE
                     ELTQLERLYLSELMTTPDAEEGIRAFLEKRTPRWSKTAVVTQG"
     gene            complement(9796..10905)
                     /gene="oah"
                     /locus_tag="VEJ00_06495"
     CDS             complement(9796..10905)
                     /gene="oah"
                     /locus_tag="VEJ00_06495"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018807 -
                     6-hydroxycyclohex-1-ene-1-carboxyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HXX00844.1"
                     /translation="MKNHNLVESPPRANILVERRPVLAQDGSPVAGLHNVWITLNNPS
                     ELNSYTTEMIKEIILALREASNDRAAVTIVLSGAGNRSFCAGGNTREYAEVYAGAPGE
                     YRQYMRLFNDMVTSVLQCDKPVICRVNGMRVGGGQEVGMACDFSIASDMAVFSQAGPK
                     HGSAPDGGSTDFLPLYVGIEHAMMSGTLCEPWSAHRFYWMGGLTQIVPVTRVDGKFVP
                     NPLVVSDRVLDEYGRLVIGEFKTGEAKKQAQDMLAEADTDLCLLDEAVDQLCTKLLYT
                     MPDCLIKTIESLRKHKLEHWNRNRETNRAWLSLNMMTEANAGFRAFHYGDKKNREADF
                     VLLRQRLAQGARWSEELIEEVAPWTVRRVTEGALK"
     gene            complement(10914..>11567)
                     /locus_tag="VEJ00_06500"
     CDS             complement(10914..>11567)
                     /locus_tag="VEJ00_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXX00845.1"
                     /translation="PGLSAESLSVVADAVTTPWEAIRRAGVGPEDVAVFVGVGGVGGF
                     GVQIAAALEAAVIAIDIDRGKLELAAQHGAGWTLDSSAMSDKDLKTAVRNFTKQSGRK
                     NIGLKIFETSGTPDGQQTAFGLIDFGGYLAVVGFTPRKVELRLSNLMALDATARGNWG
                     CPPGQYPAALDLVLRGKVAIQSYVEVHSLNDAPAVFDSVARHSITRRAILKPVANGT"
BASE COUNT         2335 a         3314 c         3433 g         2485 t
ORIGIN      
        1 accgctcccg cgactctgga ctgcgcggga gggacgccgt atcgatcgcc catacgtttc
       61 aggaatatgg tttcgtagct gccatagtgg atcaatactg gatctgtgat agtcgtgagg
      121 atgcctataa attcattcca gatctttctt tcgtcttcga cgctgtctgc ccaaagacta
      181 tactgaacag ctgaattgcg atctcccacc cgcacaccga ccaggtagta gaaatcgcgg
      241 tctggcagac cttcgacgtc aagatacact ggagtacctt caattactat tttcggattg
      301 ccgacgatgt ggattttgtt ctgccgaatc gccagcgccc tgagcgagtg gtgatacttt
      361 tcccgtttgt cgcgcacctg tttgggacgc cgacgaggac ggaaggtata ggacaattgg
      421 gtcacagtga agatcccttt atcatgcagt ttcttccgct ctttctccgt cattcccgac
      481 aatagactca gatcatcatt ctctagtgcc ttctgtcggc accgggactg aaactcgcaa
      541 tctgcacagt gtgggatcag aacgaggtct ggcggtgaag ggctagacaa caaggcatca
      601 attttcgcaa tatgctgcct cacctcaagc tccagggcgg aagtacatac atttaatgtc
      661 gcatggttat caccgtggat tattttgccc aggccgacct cttgttttag cgtctctgaa
      721 agcgctagcg cgtcaaatgc tagcagcagc ttggcgtcct tgctgatctt gttagtggaa
      781 ataaagcgag tgacgatgat gtgagcgggt ttgccgcgcc tcgctgacgg taagcactcc
      841 actgcgtgaa tgtaggattc cagattcggt gtttgcgcaa gaaccccggt ggacaaccgc
      901 cacttacacg ctttcaggtg gtctgcagaa ggggaaagca tgcactcact ctgaagcgtt
      961 ctcgagagca gccggcgaat ctcagctgca acatatgact cattccgagc ctgagtgcat
     1021 tggacgcatg tgccgtctgt aatctgctca ccggctgacc ttagccagca tttagtaggg
     1081 catttgaggt aggcttctag gaggcaggaa gagattgtca tggcttcacc ttttgtcgcc
     1141 gatgtgtcgt accggcagag cctgatagta tgagtgatta cgtatggtaa caacacctga
     1201 gtgggcagag ggaattgatc tcccgagcag tggtttttag catggcttga tgattgaaag
     1261 aggagaagcc gtggttggtt ctcctgaggc tgtgtcgtcc tagaatcccg tatggcgcca
     1321 ttattaccgg acggtgggca tgggcagggg ctcagagcta tactctcatc tctctcgccg
     1381 tccacaattg ctgcaccgtg tgccgggtga actgtagcca gtgttatagc aatatgggca
     1441 atacctcgga ggcgagagac ccaaacggaa acgttcgcgc cagttccagc gcggttttaa
     1501 agctgctgcc tgtaccctcc cgcacgaact tggtgttcgg ccgtgcctgt tgcgaagccg
     1561 gtctttccat gattcgctgg ggtaccgctt gtgtttacca tattgttaac cgcaaccgct
     1621 tgtggggtgc atattccctc ccgtcgtgtt ctaaccccgt caaaccctga ccgggatgga
     1681 aatccccgct ccctcgatcg gcggtcattg ctctagcgca acgacggaat cctgcatcat
     1741 ctgtcatcct ttcctgtgaa ggatgagggt agacgtgtag gagcggcatc gagcgcagcg
     1801 ctgccgcgag gttttcattc aagacttctt caggggctca aaccatctga aattggagca
     1861 gttcttgctc tcgcgagaca gcagagaatc tcccttagtg aggttttaca aaacgaaggt
     1921 gataccgcga tccatttgtc tttgatggtg aggggattcg ctgccttcta caagactaca
     1981 cccgagggca ggaagctctt tcttcgctgg attgcgcccg gcgacgcatt cggcctggcc
     2041 gcgctactgc aaatccaaca accgtatttg atgactatac acgcaatgag cgagggttgc
     2101 ctctttgtgt gggaccgagt gtccgcacag gctctcgttt cacaaatccc gcgcctacgt
     2161 gagaatgcct atacgatcgc gggcgactat gtggctagcc tctctgatgc actgacggcg
     2221 cgctccagcc aaactgcgca acaaaggatt gcccgagtgt tactcgagag tgcccggcag
     2281 acaggtcacg ctgggacccc aggcatcgaa cttgctctca cgaatgaaca gttggcggag
     2341 atggcagatg tgaccctgtt tactgtgagc cgtcagctta acgaatggca gaatcagggt
     2401 atcctcaaaa agagccggcg caagatcctg ctccgcgccc cgaatcgact cagttcataa
     2461 aatctacgtt gtgagaacgg tcagttaccg cgctgtctgc ccctgcggag ctggattatg
     2521 tgaggcagcg gtgctaccac cgtctgcgcc ctagcctgat cggcttggct gctgcctcgt
     2581 actccgcttt gatctgagcg gcccggatac ttgccgaggt gaggtccctg gcttctatta
     2641 tgcaactgaa ggatgccgag atttttagcg atttggcaac ccgcgaactc aaggcagaga
     2701 agggattcga gcaaaagagc gggcggaccg gcgttttcca gcggccgccg ggatacatcg
     2761 cggagagtct tggcaagaac ctcgtcgggg cctgccattc cccggcgccg tttccgcggt
     2821 gcccgtgtgt gcgatggtgg ctgcgggacc taagagcttc accgctctgc tggaaggata
     2881 aaccccttgc taagcccctg ccgttttgtg aggcggcaat tgacttaggc ggggcggcat
     2941 tggaggaatt tgctggcttc gcgacaattt tgtctacact ttcggacagc accttcgtta
     3001 ccaaattgtt gtcattccga ccgagcgaag cggaggctga ggaatctgct tctgggcttg
     3061 tgagcggcca gttcccgtca agctgtgacc atcttcgccg accagctctt tacgaatgct
     3121 ccgaagcggt cgagcgcctg cgagaaattg tctccggcgg cggcgaagcc taggcggaaa
     3181 tgcgagggcg tatcgaagca atcgccggga gccagcagga ttccacgctc ggtggcggcc
     3241 tggcagaacg ggcggtcgtt ctctccgctc agcagccagg ggaacgcggt catgccgcct
     3301 tgcggtggaa tccagccgag tacgtcgcga tggtccgcca tgaagtgctc gaggagcttg
     3361 aggtttcggg tggcggcttc ctgggtctta ccgagcacga cgtcacgttt gcggattgcg
     3421 atttccgaaa gaatctctcc ggtcgtggaa ttgcagatcg agaagtaggc gcgcgcggtc
     3481 cagtattgct ggcggcgctg ggcgtcgtgc tcgatcatcc accccgtgcg cacgccggca
     3541 atcgaaaatg ccttcgacag gtctgcgatc acggtggcgt gtggcaggcg cgccgccgat
     3601 ttcgtttgct ttccgtagta gatgggatgg taaacctcgt cactgaccag ctggattccg
     3661 cgctcggcgg tgaagtcgtg cagggcttcc atttcttcgt cgtcgatggt cgtgcccgtg
     3721 gggttatgcg ggctgttgag caggatgagc ttggtcttcg agtcggcgag ccgtttgatc
     3781 tcgtcggggt cgatgcggaa gccgttctcg cggcgcacgc ggtagaagcg ggtttccagg
     3841 ccaagagatt ccgggagcgc cgagaaagta gtgaatcctg gcagcgggat gattacgttc
     3901 gcgccgggct cggcggccag ccacatcagg gccacgagcg cttcggaggc gccggtcacg
     3961 atttggacgg cgtctaccgg gacacgctgc atttcagcga tcgcctcacg aagactgtcg
     4021 gcgccggccg ggcggctgta gaccagcttg tggttcagga agcggtagtg cgtttcgtcg
     4081 tcggccaggg tgaggatgtc gtttacggtc caggtcggtc cggtgctcgc agcgaggttg
     4141 aattctatgt cgtgttcgta ctggtcgagc caggcatcga gcaggaaggg cttgagttgc
     4201 atgcgagcac ctcgaggcag aagttggttc ctgttattga ttctgcgaat cagcgtacgg
     4261 atgattttta ggttacgagc gatgagaatg cagcgcgttt gtggcaaacc tacgttggcc
     4321 ggcctcagcc ccaaaagggg gtggtagaag cggttccctg atacaaacca tgtcgttccc
     4381 cacagccctc ttgacacccg cctgagcaaa gaatatcaag attgcttcgc tggcgttgaa
     4441 ggtcaccaag gttgtgtgga cgatggtcgg agccaagacg gcttatcatt cgcagcggcg
     4501 attgtgcggt gtctcggttc ctacctggga gtgaaaaggg aattgcatga cttgggcatc
     4561 gcctatcctg cttccggatg aattcaatgc cgcggcattc tttgtcgatc ggcacatcgg
     4621 ggaaggccgc gcgggcaaga cggcaatcat atgcggcgat gtccacgtga cctatgggca
     4681 gctctctgtt cttgtaaatc agttcggcaa tgggctgaag aggttgggga tacgcatgga
     4741 agaacgcgtg ttcctgctgt tgctggacac gccggagttc gcggtcagct tttttggcgc
     4801 gataaaaatc ggagcggtac cggttccggc caacactctg ctgaagcctt ccgactacaa
     4861 ctacctattg aataattcac gggcccgagt cgccatcgtc aacaactcct tactcccgct
     4921 gctcgagtcg attcccaaga gtgcattgcg ctatctggag accgtcgtgg tcgtgggcgg
     4981 ggcagccgtt tccggaacga tctcgttcgg cgacctaata cgcaactgct cgtgcgatct
     5041 ggacccggct ccaacgggca aagatgatgc ggcgttttgg ctttactctt cgggcagcac
     5101 aggttcgccg aaagcttgcg ttcatctgca gcatgacatg gtggtgagcg ccgagcgcta
     5161 tgctaaggcg attctgaaga ttacggaaga agaccgcttt ttcagcgtgg ccaaactttt
     5221 cttcgcctat ggactgggga acggacttta ctttccattg accgttggcg gcaccagcat
     5281 ccttttgccc gggccaccgc gaccgcagag cgtattcgag gcaattgagc gcgataagcc
     5341 gaccctgttc ttttctgttc cctcgaacta cgtcaaactg ctggctcatc ctgccccggc
     5401 ggagcgggaa ttcgaccttt cttccattcg tcacgccatt tccgccggag aagctcttcc
     5461 ggcttccgtt ttttgtcgct tcaaggaacg tttcggtgtg gaaatactcg atgccattgg
     5521 ctccaccgaa gcactccaca tgctgatttc caacgttccg ggagccattc gaccaggctc
     5581 cagtggcaaa gtcatccccg gattcgaggc caagctcgta gacgataatg atcaacctgt
     5641 cgccccgggc gagattggca acctgatggt gaaggcagac tcgacttgcg cctattactg
     5701 gaacgagcac gagaaaacca agaatacatt taccggccaa tggctgcgca cgggtgacaa
     5761 gtattatcag gacgcagatg gatacttttg gtatgcgggc cgatcagacg acatgctaaa
     5821 agttagcggc gtttgggtca gccctattga aattgagcgc gttctgagcg agcatcccgc
     5881 ggttttggag gtggcggtgg tcttccgcca ggatgcggac gaactctcca aaccagcagc
     5941 ctgcattgtg ctacgcgatg gggcgagcgc cagtcttcaa atggcgtgcg agctgcagga
     6001 atttgtaacc gaccggttgc cgatttacaa gcggccgcgt tgggtcgagt ttatgcgcga
     6061 acttccgaag acagccaccg gaaagttgca gcggtacaag ctgcgcgaaa ttgcaaccgc
     6121 aggcgcgagc ggtactgttg aagagtagcc atgagcgatc acgacgaaga tcaaagactg
     6181 ggaatgaacc gtcccgcgga cgacggaatt gaccgtcaca cgggttagca cttttatgcc
     6241 cctttttcaa ttgccgaagg aaggacgttg acagttactt agggagagaa gccgcgaacg
     6301 atctccagga tctccccgtc tgtcaacacg cgcggtgact tcttggcttc gtccatgatc
     6361 ctcttcgtta gctctggcgt caccggcacg ccgtgggaag cgagataatg ttgcacgttt
     6421 gagtttccag acatcggacc gatctcaatt ttctgcgaac ggccgatcca tcccgcgggg
     6481 acaccggaat aaattcggtc ggcaagccag gtatgcccct tcttgtgagc tttgataacc
     6541 gccgcggcgt gcactccggt ggctgtccgg aaggcatcgt ggccgacgat cggagcgttg
     6601 accggaatcg gaacccgcgt gacctgcgag accagctgaa cgtactcggg caatcgcgtc
     6661 aaatcgtcat tgcggatacc gagtagcttc agattgacga gaatctgttc gatcggagtg
     6721 tttcccacac gttccccgat tccgagagct gtgccgtgca ctcggctcgc accagcctca
     6781 atggcaacca tggtgttagc caagtcgaga ccgcgatcac ggtgcccatg ccagtcgatg
     6841 ccgacttgcg gagaaccgat ctcgcacaac aaagctccga cccaggttac gaggttgtag
     6901 acggcatctg gagccgcggc gccacaggtg tcacaaagac aaacccgctt ggccccggcc
     6961 tcaaccgccg ccttcagcag gatgcgcagt tggtccggcg aggagcgaac tgtgtcctcg
     7021 gtgacgtaca taacctcaag accttcgcgc agggcaaacg tgatggcttc gcgggaatgg
     7081 cgcaagatgt cgtcgagttc ccattcttcg gcgtactggc ggatagggct ggaaccaatg
     7141 aacaagcagg cttcgatggg cacacccgtt agctgagtag cctcggcgat cggacgaata
     7201 tccaccatca gcgtgcgcgc cgcacagttc gcccggatac gcatgtggtg ttgcgcgatc
     7261 tcccggcaaa gctgcaacac cgcttcccgc tgacgctcgc ctgcaccagg caagccaacg
     7321 tcggcggttt caatgccgag actctccatg aggtgaagaa tctgcagttt ggtgtcgatt
     7381 ggaggatcgg ttacggaagg gctctggagg ccatcgcgca gcgtctcatc gtcaagcatg
     7441 acatgaccgg gccaccgggc gcggcgcggg ccctgttctg tccagtcgta gacgaggctg
     7501 ttttcttccg gaatgcccat ggccgcatcc gctggttatt tcgaacccac cggttgtgac
     7561 ttcgcgaagc tgcgagcgcg ttgccgcaac cgctccgcgg agtcgtggtc gccctgcgcg
     7621 gcgcgaacca gagcaaggtg atacaggctg ctgacgtcgc cgctggattc ttccaggctg
     7681 tgctcgaact cgcgtgccgc cgcttcgtat tcaccttgcc gctcatgaat gatgcccaga
     7741 tggtaatgcg ccatcaggta ctcgggagtc agcgtcagcg atagctcgaa gtgctcccga
     7801 gcttcgtcga ggtggccgtt atggaacaag gcgagagcga ggcgatagtg agccgcgttg
     7861 tagttggggt cgatttcaag gcagcggcgg aaggcccgca tggcctgcgg catgtcaccg
     7921 cgatggtagt aggcgacacc gagatcgtag tatccgagag tgaaactggg cgctgccgcc
     7981 accgccttct cgtagtaccg cgcggcctgg tcaagcaagc catcatgaaa gtggagcgtg
     8041 cccagcaaat ggttggcgag cacgtggtcc ggctcggtgg tgagcaaacg ttcgagctcg
     8101 cggcgggcct cctggctgcg cccttcccgt tcgtggacca tggccagcag tagccgctga
     8161 tcaaccgcgg catcacgcag gaacaatccg cactcggcgc aaaacttgtc tggctcggca
     8221 gcgacgcgtc cgcatctggt gcaacgaaga gagttgaaat tcgtcatgag tgcctccaga
     8281 aaactaggtg gggcccagga attgcccacc atccacccgc aaaacttgtc cggtaatatg
     8341 tcctccaccg gggccacaaa ggaacgtgac tgcgtgaccg atatcttcgg gctcaacgaa
     8401 ggtgccaacc aagctttctg ccagggccgc gtcgcgaatc ggttgcggga cagagtcggt
     8461 cagcggtgtg cgcacccatc ccggctcgat cacgttgatc aggacgccga agcgtcctgc
     8521 ttcccgggct acggttttcg ccaggcccag cagaccggcc ttgctggcgg agtacgccga
     8581 tgtgccgaac ttgcctcggg aaccgttaat agacccgatg agcacgatcc ttccgccccg
     8641 acgcacccgc atccgaggca agctatgtcg aataagcaga aaagcgccgc gaaggttgac
     8701 actttgcacg aagtcccaat cgtcgaggga aagtttccaa acgaccgcat cgcgtgagac
     8761 cccggcagcg tgtaccgcga tgtcgacgcc tccctgcttg ctggtaactt cgctgacggc
     8821 atgtgccacg gaggagtcgt ctcgcacatc acattcgatc caggggagag ccaagccttt
     8881 gggcgtagcc acatccagca gcgacacgta agcgcccgcg gcctggaggg cacgggctat
     8941 cgcttggccg atgactcccg agcctcccgt caccaccgcg attctgccgt ttaggtcaga
     9001 agcctggttc atctcatcct tgggtcacaa cggctgtttt gctccaccgc ggggtgcgct
     9061 tctctaggaa agcgcgaata ccttcctccg catcgggtgt cgtcatcagc tccgacaaat
     9121 acaaacgttc cagttgcgtc agttcctccc ccaaggcacg acgcaaggac atgcgagccg
     9181 cccggcagcc gaactgcagc gaagagggcg agcggggcag aaagtccgat tccaaccagc
     9241 gctcgatctc cgcgtcaaga tcctcggcca ctcgggtgac cagtccgctg cgtgcggcct
     9301 catcggcggt gatggccgcc ccggtcagca gcaaccgcgc agccaaggcc tgacccacac
     9361 gcagcggcaa caaagccgag gcggcgggag cgaacacggc cagcttgatt tccgggacgg
     9421 caaactgcgc cgtgttgtcg gcaacgatca agtcacaagc cagagcgagt tcaaatcctc
     9481 cccccaggca ctggccgcgg acggccgcaa tcacgggcgc gggagcctca tggagccgcc
     9541 tgagcagagc gtggagcgcc cgtagagtgc cagcaatctg gtcgggcaga tgttcttgca
     9601 cgctggcgcc aaagctgaag tgcggaccct ccgcggtgag gacgagcgca ttcagggggc
     9661 gccgggcaca ctgggacaca gcctcttcga gctcgcggat catcgcccgg tccagtatgt
     9721 tggccttcgg agacgccagg gtgatgcgcg ccacccgctc ctggtgagtg aacttcagcc
     9781 ggatcttctc gaagctcatt tcagcgctcc ttccgtgaca cgccgcaccg tccagggggc
     9841 aacctcttcg atcagttcct cactccagcg cgctccttgg gccaggcgct ggcgcagcag
     9901 aacgaaatca gcttcgcggt tcttcttgtc gccatagtga aacgcgcgga atccagcgtt
     9961 ggcctcggtc atcatgttca gcgagagcca tgcacgattg gtttcgcgat tgcgattcca
    10021 atgctcgagt ttgtgcttgc gcaggctttc gatggttttg atcaagcaat caggcatggt
    10081 gtaaaggagc ttagtgcaca gctgatccac ggcttcgtcg agaaggcaaa ggtcagtatc
    10141 ggcctcggcc agcatatcct gcgcttgttt cttagcctcc ccggttttga attcgccaat
    10201 cacgaggcga ccgtattcgt ccagaacgcg gtcgctaacc accagcggat tgggcacgaa
    10261 cttaccatcc actctggtca cgggaacgat ctgagtgagc ccccccatcc aatagaagcg
    10321 atgggcgctc cagggctcgc acagcgtgcc gctcatcatc gcgtgttcga tgcccacgta
    10381 gagcggcagg aagtcggtcg agcctccgtc cggtgcggaa ccatgtttgg gtccggcctg
    10441 gctgaaaacc gccatgtcgc ttgcgatgga aaaatcgcac gccatgccga cttcctgccc
    10501 gcccccaacc cgcatgccgt taacccggca gatcaccggc ttgtcgcatt gcaggaccga
    10561 cgtgaccatg tcattgaaga ggcgcatgta ttggcggtat tccccgggtg cgccagcgta
    10621 gacctcggca tactcccggg tatttcctcc cgcgcaaaag gaacggttcc ccgcaccgct
    10681 caggacgatg gtcaccgcag cacgatcgtt ggaagcttcg cgcaaggcca ggatgatctc
    10741 cttaatcatc tcggtcgtgt agctgttgag ttcggagggg ttgttcaggg tgatccagac
    10801 attgtgcaag ccggctactg gcgagccatc ctgcgccaag accgggcggc gttccaccag
    10861 aatgttcgcg cgtggtggag attcaacgag gttgtggttt ttcatggttt ctcctaggtg
    10921 ccgttggcga caggtttgag gatggcgcgg cgtgttatgg aatgtcttgc caccgagtcg
    10981 aaaactgctg gagcatcatt gagcgaatgg acttcaacgt aagattggat agcaaccttg
    11041 ccgcgcaaaa cgaggtcgag agccgccgga tactgacccg gcgggcaacc ccagttgccc
    11101 cgcgccgtgg catcgagcgc cataagattc gacagacgca gttcgacttt cctgggcgta
    11161 aatccgacta cggccaggta cccaccgaaa tcgatcaacc cgaatgcggt ctgttgcccg
    11221 tctggggtcc cgctcgtttc aaagatcttg agaccgatat tcttgcgacc gctctgcttg
    11281 gtgaagttcc gtaccgcggt cttgaggtct ttgtcgctca tcgcagaaga atcaagcgtc
    11341 cagcccgctc cgtgctgcgc cgctagttcc agtttgccgc gatcgatatc tatggcaatc
    11401 actgcagcct cgagcgcggc agcgatctgc actccaaacc cacccacccc gccgacgccc
    11461 acgaagaccg ctacatcttc aggtcccacg cctgcacggc ggattgcctc ccagggtgta
    11521 gtgacggcat cggcaaccac cgaaagcgat tctgcactca gtcccgg
//
LOCUS       DASPUL010000261         5234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_186307, whole genome shotgun sequence.
ACCESSION   DASPUL010000261 DASPUL010000000
VERSION     DASPUL010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186307"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VEJ00_06505"
     CDS             <1..977
                     /locus_tag="VEJ00_06505"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXX00846.1"
                     /translation="SFDPAMRKAALLYNPDSGGSGRRRLKELESALQVLRAGGVEADL
                     VPTESRHHAGDEARQAVGAGCDTIFACGGDGTIHNIAQVLAKTTVALGILPMGTANAL
                     AHDLGLPVNIRAAAEAALHGRLRRIALGCVKYTSFQGTERTRYFVVAAGMGVDAHLFY
                     KLHTGAKQRMGMAAYYAKAWNLWFTYPMTRFVAEYVGDGAAEATRVDVTELLSVRIRN
                     FGGVIQELAPGASLERDDMRLVLCRTASRMAYLAYVTRGLLRRRWKIRGIELVYSTRV
                     SCNYAASSLAPVPQKVYVEADGELLGTLPAEITVVPDALTLLVPSCKT"
     gene            1103..2188
                     /locus_tag="VEJ00_06510"
     CDS             1103..2188
                     /locus_tag="VEJ00_06510"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXX00847.1"
                     /translation="MEPVTHFLTGACLGRAGLNRKTGLATLTLILAAEAPDLDVLGRF
                     RGPAFGFAHHRGFTHSFLGVPLTAAVVVGFVYLVWRMRGRKTKDPNLPPRWGVLFGYA
                     CLAGLSHILLDFTNNYGVRPFWPFYEKWYSWDIVFIIEPVMLALLLLGLIVPSLFSLI
                     DREIGVRHRRPRGRVAATAALVGVVLMWTVRDYEHRRAVSTLEARTYNGADPLRVSAY
                     PKWVDPFHWYGVVETPTFFALTPVDSIEPEVDPNGEMEIRYKPEETPVTLAAKNSYLG
                     RVYLDWAQYPVTETEKLTDGGYIVHLVDLRYTQIPSAISRDRGRRVLGAAIEVDKNLH
                     VIGDVYDERDDNGTLRRRTVVPEPGQR"
     gene            2316..2876
                     /locus_tag="VEJ00_06515"
     CDS             2316..2876
                     /locus_tag="VEJ00_06515"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HXX00848.1"
                     /translation="MLPYLFVLLAVAFRFLPFAGHLNVLPHAWHFTPLAASLLFFGAR
                     GSRRQMWIPLVLLIATDVVLTKFVWDYSFAWDVIVSWVWYAAILWLGTNLREKAGFWR
                     VVGAAAASSVSFFLVSNFGSWVAWPQMYPRNFSGLMMSYTAGIPFFRGTAVGDMFFSL
                     AMFGTPVAIRALSGWLHRSDDHMASA"
     gene            complement(2887..3513)
                     /locus_tag="VEJ00_06520"
     CDS             complement(2887..3513)
                     /locus_tag="VEJ00_06520"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HXX00849.1"
                     /translation="MKTIGHILARYMAWILGLLKVLGIWGAFVIAFADSALLGMPIDA
                     IVATYVYQDRKRLLFYIAMASLGSALGSIPLYIIGYQGGERVLRKRISEERFQKIHQS
                     FERHEFWALMFPGMLPPPMPFKIFVLGAAVFEMRFSHFLAAIFAGRFVRFLVLSILVL
                     WFGPQIVGIVGGFFKQHFYWLAGLLVVLAALGLMARRRRIRSARLEES"
     gene            3654..5132
                     /locus_tag="VEJ00_06525"
     CDS             3654..5132
                     /locus_tag="VEJ00_06525"
                     /inference="COORDINATES: protein motif:HMM:NF006560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXX00850.1"
                     /translation="MRKLILSLGLLLLPSVLAGQQYDLVIEGGRVMDPETGLDGVRNV
                     GIRDSKIARVSADTMNGRRVIQAAGLVVAPGFIDLHQHGQELESQRVKAFDGVTTALE
                     MEIGVPDVGQFLRRKEGHSLIHYGTTASHAAARALVFGAPLNKESSDSRVGIREILPK
                     SGPATNQPATPDQIKRIEQRLQDELDAGALGIGMGIQYTPGATRLEVIDMFRLAAGRK
                     LPVYTHVRSFGRTEPGSDIESVEEVIGAAAITGASLHVVHINSTCLADSLECLSLIDG
                     ARARGLDVTTEAYPYIAGMTEINSALFNPGWREKLGIDYSGLVLPDTGEHLTKERFEE
                     LHKSSTPQSVLVFANTQEMVDEVIPNPLVMIASDGAQGHPRNAGTYARVLAEYVRGKK
                     TVALMEALRKMTLMPAQMLERSTPEGRLKGRLQEGADADVVVFDPQTIRDRATFEKPM
                     ETSVGVRYLLVDGTVLIDDGKMAADVYPGRAVLGPGKARRTN"
BASE COUNT         1024 a         1455 c         1684 g         1071 t
ORIGIN      
        1 ccagcttcga tcccgccatg cgcaaggccg cgttgctcta caatccggat tccggcggga
       61 gtggccgacg gcgcctgaag gaattggaat ccgcgctgca ggtgctgcgt gccggcggcg
      121 tggaggcgga tctggtcccg acggagtccc gacaccacgc cggagacgag gcccggcagg
      181 cggtgggtgc gggatgcgac acgattttcg cctgcggcgg cgacggaacg atacataaca
      241 tcgcgcaggt actggctaag actacggtgg cgcttggcat tctgcccatg ggaacggcca
      301 acgcgctggc gcacgatctt ggcctaccag tgaatatcag ggctgcggcg gaggcggctt
      361 tgcatggcag gctccgccgc atcgcgctgg gctgcgtgaa atacacaagc tttcagggga
      421 ccgaacgcac acgctatttt gtggtcgctg cgggaatggg tgtggacgcc cacctgttct
      481 acaagcttca caccggggcc aagcagcgca tgggcatggc ggcctattat gccaaggcct
      541 ggaatttgtg gttcacgtac cccatgacgc gatttgtggc ggagtacgtg ggcgacggag
      601 ctgccgaggc cacgcgagtg gatgtcacag agctgctgag cgtgcgcatt cggaacttcg
      661 gcggggtgat ccaggaactg gccccgggag cgtcgctcga gcgtgacgac atgcggctag
      721 tgttgtgccg cacggcctcc cggatggcgt acctcgccta tgtgacgcgg ggactgctgc
      781 gccggcggtg gaaaatccgg ggcattgaac tcgtgtacag cacccgagtt tcatgcaact
      841 acgcggcttc ttcgttagcg ccggttccgc agaaggtcta tgtagaagcg gatggcgaac
      901 tgctggggac gctcccggcg gagattaccg tcgttcccga cgcgctgacg ctgctggttc
      961 cttcttgcaa aacgtgatct tcgccgagcg aatttctttg cgacttcgtt tctgtcgcag
     1021 agtcgaatta acgtcgcaga atccttgcag gggcgcggac tggcgatagt caagcctggc
     1081 gcaatagaat tctccgaacc gcttggaacc cgtcacccat tttctgaccg gagcatgcct
     1141 gggtcgcgcc gggcttaacc gcaaaaccgg actggccacg ctcacgctca ttctggcggc
     1201 ggaggctccc gatcttgatg ttctggggcg atttcgcggg cccgcgtttg gattcgcgca
     1261 tcaccgcggc tttacgcatt ccttcctggg tgtcccgctc acagcggctg tggtggtcgg
     1321 attcgtctat ctggtgtggc gcatgcgcgg ccgcaagaca aaagatccga acctgccgcc
     1381 acgctggggc gtgctgtttg ggtacgcctg cctcgccggg ttgagccata tcctgcttga
     1441 tttcacgaac aattacggcg tgcgcccttt ctggccgttt tatgaaaagt ggtactcgtg
     1501 ggacatcgtt ttcatcatcg aaccggtgat gctggctctg ttgctgctgg ggctgattgt
     1561 cccttcgctt ttttcgctga ttgaccggga aatcggcgtg cgccaccgcc ggccacgcgg
     1621 gcgcgtggca gcaacggcgg ctctggtggg cgttgtgttg atgtggacgg tgcgcgacta
     1681 cgagcatcgc agggcggtca gcactctgga agctcgcacc tacaacggcg ccgatccgct
     1741 gcgggtctcg gcttatccga agtgggttga tccatttcat tggtatggcg tggtagagac
     1801 gccgactttt ttcgcgctaa cgcccgtgga ttccattgaa ccggaagtgg accccaacgg
     1861 ggagatggag atccggtata agccggagga aactcccgtg acgctggcgg cgaagaattc
     1921 ctatctagga cgggtgtatc tggattgggc gcaatacccg gtcaccgaaa cggagaagtt
     1981 gaccgatggg ggttatatcg tgcatctggt ggacctgcgc tacacgcaga ttccgagcgc
     2041 gatcagtcgc gaccggggaa ggcgggtgtt aggcgcagcg atcgaagtcg acaagaatct
     2101 gcatgtcatt ggggacgtct atgatgaacg ggacgacaat ggaacgctac ggaggcggac
     2161 tgtcgtaccc gaacctgggc agcgttgagc gtcgatctgg tcgtggggtg tcgagctttc
     2221 gaccgacaaa cggaatagtt accgccgatt ccgtgcttgg tcgcttgacg gcggctttta
     2281 taacgcccaa aatgatcttt cgtcgtgagg tgatcatgct gccctatctc ttcgtactgc
     2341 tcgccgttgc ctttcgcttc ttgccttttg ctggacatct caacgtgctg cctcacgcat
     2401 ggcatttcac gcccttggcc gcctctttgc tcttcttcgg agcacgcggc tcgcggcgcc
     2461 agatgtggat tccgttggtg cttttgattg cgactgacgt ggtgctgacc aagttcgttt
     2521 gggattactc tttcgcgtgg gatgtgatcg tcagctgggt gtggtatgcg gcgatcctgt
     2581 ggttgggcac gaatctgcgc gagaaggccg ggttctggcg cgtggtgggc gcggctgctg
     2641 ccagttccgt gtcgttcttc ctggtcagca acttcggttc atgggtggca tggccgcaga
     2701 tgtatccaag aaacttcagc ggcctgatga tgtcatacac ggccgggatt cccttcttcc
     2761 gcggcactgc ggtcggcgat atgtttttct ctctggcgat gtttggcaca ccggtcgcga
     2821 ttcgggcgct ctccggctgg ctgcatcgat ccgacgatca catggcatca gcataggatt
     2881 caggtctcac gattcttcca atctcgccga acggattctc cgccggcgcg ccatcaggcc
     2941 cagggccgcg agaacaacga gcaagccagc cagccagtag aaatgctgct tgaagaatcc
     3001 gccgacgatt ccgacgatct gcggtccgaa ccagagcacc agaatcgaga gcaccaggaa
     3061 gcggacgaag cggccggcaa agatcgccgc caggaagtgg ctgaaccgca tttcaaacac
     3121 ggccgcaccg agcacaaaaa tcttgaacgg catggggggc gggagcatgc cggggaacat
     3181 cagcgcccag aattcgtgcc gctcaaacga ttggtgaatc ttctgaaagc gctcttccga
     3241 aatacgcttg cgcagaactc tttccccgcc ctgatacccg atgatgtata ggggaatact
     3301 gccgagcgcc gagcccagcg atgccatggc aatgtaaaag agcaggcgtt tgcgatcctg
     3361 gtagacgtac gtagcaacga tggcatcgat cggcatgccg agcagggcgc tgtcggcgaa
     3421 ggcgatgacg aaagctcccc agatgccaag caccttgagc aaccccagaa tccacgccat
     3481 gtagcgcgcc agaatgtgtc cgatcgtctt caaggaaaca gctagtgtac gggattgaag
     3541 ggcatgtgtc agcgcggcag aggtggccag cagtgttctt gctgatacga agacttggag
     3601 tgaacgcccg cctctcttcg ttgcctgata tcatcgctgg ctttggagtc gctatgcgaa
     3661 aactaattct ctccctgggc ttgctcctgc ttccgagcgt tctcgcgggg cagcagtacg
     3721 atctggtgat cgagggcggg cgcgtgatgg atcccgagac cggattagac ggcgtgcgca
     3781 atgttgggat ccgggatagc aagattgccc gggtgtcggc cgacacgatg aatggccgcc
     3841 gtgtaattca agccgctggt ttggttgtcg cacctggttt tattgatctg caccagcacg
     3901 gccaggaact cgagagccag cgtgtcaagg cgttcgatgg agtgacgacg gcgcttgaga
     3961 tggagattgg agtgcctgac gtcgggcagt tcctgaggag aaaggagggg cattccctga
     4021 tccactacgg tacgaccgca agccacgcag cggcgcgagc gctggttttt ggcgcaccac
     4081 taaacaagga atccagtgat tcgcgggtcg gcattcggga aatcctgccc aagagcggac
     4141 cagccacgaa ccaacccgcg acgcccgatc agatcaagcg aattgagcag cgactccaag
     4201 acgagcttga cgctggtgct ctcggaatcg ggatgggcat tcaatacact ccgggggcca
     4261 cccggcttga ggtcatcgac atgttccgcc tggccgccgg acgcaaactg cccgtctata
     4321 cgcacgtgcg cagcttcgga cgcacagaac cgggctcaga tatcgagtcg gtcgaggaag
     4381 tgatcggggc tgctgccatt acaggcgcat ccctgcatgt tgttcacatc aacagtacat
     4441 gcctggccga ctcgctcgag tgcctttcgc tgatcgacgg tgcgcgggcg cgcggactcg
     4501 atgtaacgac cgaagcatat ccctatattg ccggcatgac cgagatcaac tcggcgctgt
     4561 tcaatcccgg ctggcgggag aagcttggca tcgactacag cggcctggta ttgccggata
     4621 cgggggagca tctcacgaag gagcgctttg aggagttgca caagtccagc acgccacagt
     4681 cagtactggt gttcgccaac acgcaagaga tggtggacga ggtgattccc aatccgctcg
     4741 tcatgattgc gagcgacggg gcgcaggggc atccgcgcaa cgcagggaca tacgcgcgcg
     4801 tgctggcgga gtatgtgcgc gggaaaaaga ccgtggcact gatggaagca ctacgcaaga
     4861 tgacgctcat gccggcacaa atgctggagc gatcaactcc tgaggggcgc cttaagggac
     4921 ggctgcagga gggcgctgac gccgacgtag tagtatttga tccgcaaact atccgcgacc
     4981 gggcgacgtt tgaaaagccg atggagacta gcgtgggtgt gcggtacttg ctggtcgatg
     5041 gaactgtatt gattgacgac gggaagatgg cggctgacgt gtatccgggc cgcgccgtgc
     5101 tggggccggg gaaagcccga agaacgaact agacgtttcc aagggttgct aggcaggaaa
     5161 tgcgaccacc ggggctaaaa ccccggtaag aaagctcacg atgcgcggcc ctgaagggcc
     5221 gctcttccac aaaa
//
LOCUS       DASPUL010000262         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1548489, whole genome shotgun sequence.
ACCESSION   DASPUL010000262 DASPUL010000000
VERSION     DASPUL010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548489"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1360)
                     /locus_tag="VEJ00_06530"
     CDS             complement(203..1360)
                     /locus_tag="VEJ00_06530"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXX00851.1"
                     /translation="MEGAVFRLCRVSLQACLFLMWAAFLSGQQPAETPTVPAEFNLDV
                     QTAARLRPQLLMESKPPLGPHATARLVFERLIAEVRDPEGIKVSWQLRIVESDHLNAY
                     SSPDGTVYVESGLAQLAGPSTGLWAAILAHEIAHVVRRDWARRYLYQKTLENEGGATI
                     LMGDPGVPASDWTDPQKPSQDLARFCRRLEIEADRDGLMLMARAGYHPDFVPALHHLL
                     HAQGSDAPAVSLYAMHPCWEQRDHELSRAYTEASIEFDHRWPEWYASPGGNPPIVVFA
                     EDPTVKKIGSGSWQIQVPMRCQNLAGAVEVVLRASTTQGKTARAEPLPDQPEPANEIR
                     QLTGCTSPRTTITFTLADSSAPRKPARWTDVYVLDAWGAVLARTDVPKLPR"
     gene            complement(1486..>2964)
                     /locus_tag="VEJ00_06535"
     CDS             complement(1486..>2964)
                     /locus_tag="VEJ00_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXX00852.1"
                     /translation="LPLVYCSAKPDPNTLVMIIESSPTNLDPRVGIDAQSERIDNLIF
                     DDLLSRGDDLSVAPGLAERWEIPDPLTYIFHLHRGVRFHDGRELTARDVKWTFDSLLQ
                     GKIRSTKAANYRFVDRIDAPDDSTVVFRLKEPDSPLLWNLSDGAVGIVPYGSGDEMTT
                     HPIGSGPFKFVSAETDREVVLERNDDYWGEKAKLLRVRFAVVPDETTEALELRKGSGD
                     IAINSLTPDTVLTLGRDPGLVVEHAPGTRLAYLAFNLRDPILKEVRVRQAIAYALDRK
                     PMIDYLLRGEAQLARSVLPQQSWAYDGDVPAYEHNLDKANQLLDAAGYPEVDGVRFHI
                     TMKTSTVADTRLMVAVMQQQLRQAGIALDIRSFEFATFFSDVTHGAFQMHGLRWIGGN
                     EDPDIFEYAFHSAKFPPNGANRSFYSNPKVDALIDRARRETDPSIRKPLYAEVQRILA
                     EDLPYIDLWYLDNVIVHTRRVKNLKLNPAGNYDFLRTAEVAP"
BASE COUNT          540 a          860 c          987 g          577 t
ORIGIN      
        1 ttaatgctac cacgcaccgc gggagggttg cgggctgacc gcccttctgt gttcctggac
       61 cctctgcgtt ctcagcggct tttctccacg atctccgtgt tagatagaga aatcttgaac
      121 cgcagaggtc acggagaatt cacggaggac gcaaagggga tcccggatgg acttggtcca
      181 cagacttcag ctgctacagg cgtcagcgcg gcagtttcgg gacgtctgtc cgcgcaagca
      241 ctgctcccca ggcatcgagc acgtacacgt cggtccagcg ggcgggcttc ctgggcgcgg
      301 aggaatcggc cagggtgaag gtgatcgtgg tgcgcggcga ggtgcagccg gtcagctggc
      361 ggatttcgtt cgccggctca ggttggtcgg gaagaggctc ggcgcgcgct gtctttccct
      421 gcgttgtgct ggcgcgcaga acgacctcca ctgctccggc gagattctgg caccgcatgg
      481 gaacctggat ttgccacgat cccgagccga tttttttcac cgtcgggtct tcggcaaata
      541 cgacgatcgg cggattgccg cccggcgagg cataccactc gggccagcgg tggtcgaatt
      601 cgatgctcgc ttcggtgtag gcgcggctta attcgtggtc acgctgctcc cagcatgggt
      661 gcatggcgta gagcgacacc gcgggcgcat ccgagccttg cgcgtgcagc aggtgatgga
      721 gcgcgggaac gaagtcggga tgatatccgg cccgcgccat cagcatgagt ccgtcgcggt
      781 ccgcctcgat ttccaggcgg cggcaaaacc gcgccaggtc ctgggaaggc ttctgcggat
      841 ccgtccagtc gctggcgggc acgccgggat cgcccatcag aatggttgcg ccgccttcgt
      901 tttccagcgt cttctggtag agataacgcc gggcccagtc gcgccgcacg acatgcgcga
      961 tctcgtgggc cagaattgcg gcccacaagc cggtgcttgg gcccgcaagc tgggccagcc
     1021 cgctttccac gtaaaccgtt ccgtcaggag atgaatacgc gttgaggtga tcgctctcga
     1081 cgatgcgcaa ctgccatgaa accttgatgc cctcgggatc gcgcacctcc gcgatcagac
     1141 gctcgaacac aaggcgggcc gtggcgtggg gacccagggg cggcttgctc tccatcagca
     1201 gttgggggcg cagccgggcg gcggtttgta cgtccagatt gaactcggca gggactgtcg
     1261 gcgtctccgc cggttgctgg ccggagagga aagcggccca catcaggaac aggcaagcct
     1321 gaagagaaac tcggcacagc ctgaatacag caccttccac gggggatcct ccgcggagat
     1381 tgtttctcaa gaaaattatc ctacgttttg gttctttggg atcaacaaaa acgtgcgagt
     1441 agcacctcaa tcacttccga tgcgggaacg actgtgtgca agagtctacg gcgccacttc
     1501 cgccgtgcgc agaaaatcgt agttgcctgc cggattcagc ttcaggtttt tgacccgccg
     1561 cgtgtgcacg atgacgttgt cgagatacca gagatcgatg taaggcagat cctcggccag
     1621 gatgcgctgc acctcggcgt agagcggctt gcggatgctg ggatcggttt cgcggcgggc
     1681 gcggtcaatc agcgcgtcga ccttcggatt ggaatagaaa ctgcgattcg cgccgttggg
     1741 aggaaatttg gcggagtgaa acgcatactc gaagatgtcc gggtcctcgt tgccgccgat
     1801 ccagcgcagg ccatgcatct ggaaagcgcc gtgcgtcaca tcggagaaga aagtcgcgaa
     1861 ctcgaagctg cggatgtcga gcgcgatccc ggcctggcgt aactgctgct gcatgactgc
     1921 caccatcaga cgcgtgtccg cgaccgtgga ggtcttcatg gtgatgtgaa aacggacgcc
     1981 atccacctcc gggtagccgg cggcgtcgag gagctggtta gccttgtcga gattgtgctc
     2041 gtatgcaggc acatcgccgt cgtaggccca gctctgctgc gggagaacgc tgcgcgccag
     2101 ctgcgcttcc ccgcgcagca ggtagtcgat catgggcttg cggtcgagcg cataggcgat
     2161 ggcctgacgg acccgcacct ctttcaggat gggatcgcgc aggttgaacg ccagataggc
     2221 cagccgcgtg ccgggcgcgt gctcaacgac gaggccggga tcgcgcccga gtgtgagaac
     2281 ggtatcgggt gtgagtgagt tgatggcgat gtctccgctg cccttgcgca gttcgagcgc
     2341 ctcggtagtt tcgtcgggaa cgacggcaaa gcgcacgcgc aggagcttgg ccttctcccc
     2401 ccagtagtcg tcgttgcgct cgagcacgac ttcgcgatcg gtttctgcgc tgacgaactt
     2461 gaacggcccg gacccgatgg gatgtgtcgt catctcgtcg ccgctgccgt agggcacgat
     2521 gcctacggcg ccgtctgaaa ggttccacag caaaggcgag tctggttctt tcaggcggaa
     2581 aacgaccgtg gagtcgtccg gcgcgtcgat gcggtcgacg aaacggtagt tggccgcctt
     2641 ggtgctgcga atctttcctt gcagcaggga atcgaatgtc cactttacgt cgcgcgcagt
     2701 caactcgcgg ccgtcatgaa atctcacgcc gcggtggaga tgaaaaatgt aggtgagcgg
     2761 atcgggaatt tcccagcgct cggccaggcc gggagcgacg ctcagatcgt cgccgcggga
     2821 gagcaggtcg tcgaagatca ggttgtcgat gcgctccgac tgcgcatcga ttcccacgcg
     2881 cgggtcgaga ttggtcgggc ttgactcgat gatcatgacc agggtgttgg ggtcaggctt
     2941 ggctgaacag tacacaagcg gaag
//
LOCUS       DASPUL010000263         5233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1147266, whole genome shotgun sequence.
ACCESSION   DASPUL010000263 DASPUL010000000
VERSION     DASPUL010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147266"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VEJ00_06540"
     CDS             <1..921
                     /locus_tag="VEJ00_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00853.1"
                     /translation="VREHFRRYSELEPLYLPSQPGKNPFAELIASNDPYGFARSYTFN
                     VAPVHDSAPFFFFTLKPEQFFAKTKLHKGVDWKVNLGVLVLLLVLVISLVAVVAFLVL
                     PLALKGRGQRQSPLPLLYFVAVGLGYILVEIAFIQRFVLFLGHPTYALTVVIFLLMIS
                     SGAGSLFSRRWLARAEQGWLPLMLVAATVLLYVLFLPGRLADWVGLDFAYRLLISGGL
                     LIPLGFVMGMPFPTGLRALASAPAPEFPASPDNAVEWAWAMNAAASVLGSVLAMVIAI
                     QFGLTATLACGAVAYLLALVLMPALRTKAA"
     gene            1024..2103
                     /locus_tag="VEJ00_06545"
     CDS             1024..2103
                     /locus_tag="VEJ00_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXX00854.1"
                     /translation="MPSQKQLRWSQLRVGITVIIASLTLAVLLFLMSNTAGLFTPRIT
                     IRSYFDNAQGLRVGAPVRLSGVDIGNVTRIRIVPDKDKQLTPVEITMKVSTRYIYGLR
                     RDSVTSLETAGVLGETYLDIDSSQALGPPARDGDVLPTQVHPDFNQVVRASQSTLQNM
                     DALLKRADRILAFAESGKGSLGKLIYDPTLYNRFSATVSDFQKIVQQVGSGQGSLGAL
                     ISRNDAYDKFTATLDKMNAVIDDIQQGKGTAGKFLKDPSLYDNANDTIANLKQVTADI
                     NAGKGTLGKLTKDEELAKKLDTTITKLSQLTSEVEAGQGSVGKLFKDESLYNNANQML
                     VETRDLIKAVRENPKKYLSIKLHIF"
     gene            2190..3917
                     /locus_tag="VEJ00_06550"
     CDS             2190..3917
                     /locus_tag="VEJ00_06550"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXX00855.1"
                     /translation="MLRFPHRLAVTALAVLLACCAGLSMLPCSAQDKEEAIDLNMYQQ
                     IRDEGFHRSHIMEYASGLFDGIGPRLTGSPNMKRANEWTRDQLTAMGCSNAHLEDWGE
                     FGMGWQQRNTWVRMVSPDMAVFIAQAVPWSPATNGPVSTQLVAVDAKSEKDFEQYRGK
                     LTGKVVLLGKARSSSKEDVPQSTRYDEKGLADIFRYGYTDEDLQEQHLYPLDAMEQAF
                     DGPIAFGEKLGKFLAEEKVAAVLVPGSSTGGTIHDDTNLAFGWFVYEKDRAMQVPLAV
                     VALEHFGRMARLLDAHVPVAVEVNIDTTFTGDHEHGFDTIAEIPGSDPKLKDQVVMVG
                     GHLDSWAAGTGATDDGAGAITAMEVMRILTALHVHPRRTIRIGLWSGEEQGLFGSIGY
                     VKAHFGSFLRSTTPTQLKVPEFLRKPAGPLTLKPEHKLISAYYNLDNGGGKLRGIYAQ
                     ENNAVIPIFEQWIAPLRDLDVTTVSARYTGGTDHLSFDEVGIPGFQFIQDPLDYETLS
                     AHTNMDVYERLRPADLQQAAVVEAIFVYNTAMREQMMPRKPLPHPELYEEQRKPLSNV
                     MPGAGPETK"
     gene            4113..>5233
                     /locus_tag="VEJ00_06555"
     CDS             4113..>5233
                     /locus_tag="VEJ00_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXX00856.1"
                     /translation="MAPILLWLILILCLLISVVKYLMGIIMRFSFPSAKARKDYSYEP
                     TVSVLMPCFNEGKTVYETIESISKSNYPNDKFEVIAQDDCSVDDSYEWMLKAQRDFTN
                     IRIRTGRNEVNSGKARSVCNALQHSNAEVIISIDSDCIFHPNAIRELTACFAEPGIGS
                     VGGRVGVRNPNDSVITAIQTVIYYSAFQLYKIPENWTRSICCISGCLFAIRRSLLLEI
                     EPAIRARNWFGVPVNQGEDRFLTHQTLLRGYGTYINNDALCWTTVPNTLSVLFKQQLR
                     WRRSIVRDFFFTLKTLPRHVWKLHPNTVFTLVLTPLGALVGLLVVLTALTTDPLAWAG
                     PVPLVSALAVGAVLTWVIKKYSAEEAVTHPLAFGAYVAWT"
BASE COUNT         1043 a         1432 c         1684 g         1074 t
ORIGIN      
        1 gtgagggagc acttcaggcg atattccgag ctcgaaccgc tgtatctgcc atcccagccc
       61 gggaagaatc cgtttgcgga actgatcgcg agcaatgacc cgtatggttt tgctcgctcc
      121 tacacattca acgtggctcc ggtgcacgac agcgctccgt ttttcttttt cacgctgaag
      181 ccggaacagt tctttgccaa gacgaagttg cataagggcg tggactggaa ggtgaattta
      241 ggggtactcg ttctgctgct ggtgctggtg atttcgcttg ttgcggtggt cgcgttcctg
      301 gtgctgccgc tggcgctgaa aggccgcggg cagcgccagt ctccgctgcc cctgctctat
      361 ttcgtggcgg tgggtttggg ctacattctg gtggagatcg ccttcatcca gcggtttgta
      421 ctttttctgg gacatcccac gtatgcattg accgtggtga ttttcctgct gatgatctca
      481 agcggggccg ggagcctgtt ttcgcgacgg tggctggcgc gggctgagca agggtggctg
      541 cctctcatgc tggtggcggc gactgtgctg ctttatgtgc tcttcctgcc cggacggttg
      601 gcggattggg tggggctgga cttcgcctat cggttgttga tcagcggcgg gctcttgatc
      661 ccgctggggt ttgtgatggg gatgccattt ccaaccggcc tgcgtgcgct ggcgtcggca
      721 cccgcgcctg agtttcccgc gtcgccggac aatgctgtcg agtgggcgtg ggcgatgaat
      781 gcggccgcca gtgtgctggg atcggtgctt gccatggtga ttgccatcca gtttgggctg
      841 accgcgacgc tggcttgcgg ggcggtggcg tacctgctgg cgctagttct gatgccggct
      901 ttgcggacga aagcggcata acgaaagacc caagtttgtt atcatgcagc caggagtcgg
      961 gcgtgctccc gcgccccagg cgcggccacc gacggcattc cgactgggtt ctgagggaaa
     1021 catttgccga gccagaagca gctgaggtgg tcgcaactgc gggttgggat cacggttatt
     1081 atcgcgagcc tcacgctggc tgtgctgttg tttctgatgt cgaacacggc cgggctgttc
     1141 actccccgca tcaccatccg gtcttatttc gataacgccc agggattgcg cgtgggagcg
     1201 ccggtgcggc tgagcggcgt ggatatcggg aatgttacgc ggatccggat tgtccccgac
     1261 aaagacaagc aactcacccc tgtcgaaatt acgatgaagg tgagcacaag gtacatttac
     1321 ggcttgcgcc gggattctgt aacctccctg gagactgcgg gagtgctggg agaaacctat
     1381 ctcgacatcg acagctcgca ggcactgggc ccgccggcgc gggatggaga cgtcctgccg
     1441 acgcaggtgc atcctgactt caaccaagtg gtgcgggcca gccagagcac gctgcagaac
     1501 atggatgcgc tgctgaagcg cgccgaccgg atcctggcgt ttgccgagag cggcaaggga
     1561 tcgctgggga aactgattta cgacccgacg ctgtacaacc gcttctcggc gacggtttcg
     1621 gatttccaga agattgtgca gcaagtgggc agcgggcagg gcagcctggg agcgctgatc
     1681 agccgcaacg acgcctatga caagttcacg gcgacgctgg acaagatgaa tgcggttatc
     1741 gatgacatcc agcagggcaa gggcacggcc ggcaagttcc tgaaggatcc ctcgctctac
     1801 gataacgcca acgataccat cgcgaacctg aagcaggtga ccgccgacat caatgccggg
     1861 aagggcacgc tgggcaagct gaccaaagac gaggagctgg ccaagaagct cgatacgacg
     1921 atcaccaaat tgtcgcagct caccagcgag gtcgaagcgg gccagggcag cgtggggaag
     1981 ctgttcaagg atgaatccct atacaacaac gccaaccaga tgttggttga gacgcgcgac
     2041 ctgatcaagg ctgtccgcga gaacccgaag aaatacctga gcatcaagct gcatatcttc
     2101 taacctgttc ctgcgggttg cgaaacgacg ccggctgcgt caaatcctag ataagccatt
     2161 tgcacactct tagccggagg atcctctgca tgctccgatt cccacatcgt cttgccgtca
     2221 ccgcgttggc ggtcctgctg gcttgctgtg ccgggttgtc gatgctcccg tgttctgcgc
     2281 aggacaagga agaggcgatc gatttgaaca tgtatcagca gattcgcgac gagggcttcc
     2341 atcggtcgca catcatggag tatgcctcgg gattgttcga cgggattggg ccgcggctga
     2401 caggttcccc caacatgaag agggccaacg agtggacgcg ggaccaactc acagccatgg
     2461 gctgctcgaa tgcgcaccta gaagattggg gcgagttcgg catgggatgg cagcagcgca
     2521 atacctgggt gcgaatggtg tcaccggaca tggcagtctt catcgcgcag gcggtaccct
     2581 ggtcgccggc caccaatggt ccggtgtcga cacaactggt tgcagtcgac gcgaagagtg
     2641 agaaggactt tgaacaatac aggggaaaac tgacgggcaa ggtcgtgctt ctgggcaagg
     2701 cgcgcagttc gagcaaggag gatgttccgc aatccacacg atacgacgag aagggtctgg
     2761 cggatatctt ccgctacggg tacacggatg aggaccttca ggagcagcat ctgtatccgc
     2821 tcgacgcgat ggagcaagcg ttcgacggtc ccatagcgtt cggcgagaag ctgggcaaat
     2881 ttctggccga ggagaaggtc gcagcggtgc tggtgccagg atcgtcgacg ggcggcacga
     2941 tccacgacga caccaacctg gcgttcggat ggttcgtcta tgagaaggat cgcgccatgc
     3001 aggttccgct tgcagtcgtg gcactggagc acttcgggag aatggcgcgc ctgctggacg
     3061 ctcatgtgcc ggttgcagtg gaagtgaaca ttgacaccac tttcactggg gatcacgagc
     3121 acgggtttga caccatcgcc gagattcccg gcagcgaccc gaaactcaag gatcaggtgg
     3181 tgatggtggg ggggcacctc gatagctggg ctgccggaac cggggctacg gacgacggcg
     3241 cgggagcaat caccgccatg gaagtgatgc ggattctgac cgcgctgcat gtgcatccgc
     3301 ggcgcacgat tcgcattgga ctatggagtg gggaggagca ggggctgttc ggatctatcg
     3361 ggtacgtcaa ggctcatttc ggcagctttc tgcgctctac caccccaacg cagttgaagg
     3421 tgccggagtt cctgcgcaag cccgcggggc cgctgacgct gaagcccgag cacaagctga
     3481 tttcggcgta ctacaacctc gacaacggcg gcgggaagct acggggcatt tacgcccagg
     3541 aaaacaacgc ggtgattccg atctttgagc agtggatcgc gccgctgcgc gacctggacg
     3601 tcaccaccgt gagtgcgcgc tacacggggg ggacagatca cctctccttc gacgaagtcg
     3661 gcattccggg atttcagttt attcaagatc ctctcgatta cgagacgctg agcgcgcata
     3721 cgaatatgga tgtgtacgag cgcctgcggc ctgcggatct gcagcaggcg gcggtggtgg
     3781 aagcgatctt tgtgtacaac acggccatgc gcgagcagat gatgccgcgt aagcccctgc
     3841 cacatcccga actctacgaa gagcagcgga agccgctgtc caatgtcatg cccggggcgg
     3901 gtccggagac gaagtgaagg cctggctcgc gaaagtacct tgagagtcaa ctgcacaccg
     3961 ctcggagcca tttcgtattc tgggggcgtc tgtaaagatc tgtagagaag cttttccacg
     4021 atcttcagtg cgagagcgtt tgctgctcat cgcaaaactc cagtgctgaa gcggggttct
     4081 attgagcgag acctcgggag ggcgtgccgg gaatggctcc cattcttctg tggctgatcc
     4141 taatcctttg cctgttgatc tccgtcgtga agtacctgat ggggatcatc atgaggttct
     4201 cgtttcccag cgccaaagcc cgcaaagact atagctacga accgacggtg agcgtgctga
     4261 tgccctgctt caacgagggt aagacggtct acgagacgat tgagagcatc agcaagagta
     4321 actatcccaa tgacaaattc gaggtgatcg cgcaggacga ctgctcggtc gacgacagct
     4381 atgagtggat gctcaaggcg cagcgcgact tcaccaacat ccgcatccgg acggggcgaa
     4441 atgaagtcaa cagcgggaag gcgcggtcgg tctgtaacgc gctgcaacat tcgaacgccg
     4501 aggtgatcat ttcgatcgat tcggattgca tctttcatcc gaacgcgatt cgggagctga
     4561 ccgcgtgttt tgccgagccg gggattggat cggtgggcgg acgcgtcgga gtccgcaatc
     4621 cgaacgacag cgtgattacg gcgattcaaa cggtcattta ctactccgcc tttcagcttt
     4681 ataagattcc cgagaattgg acgcgcagca tctgctgcat cagcggatgc ctgtttgcca
     4741 tacggcgctc gctgctgctc gagattgagc cggcaattcg cgcgcgaaat tggtttggcg
     4801 ttccggtgaa ccagggcgaa gaccgatttc tgacgcatca aacgctgctg cgcggctacg
     4861 gaacctatat caacaacgac gcgctgtgct ggacgaccgt gccgaacaca ctgtcggtgc
     4921 tattcaagca gcagctgcgc tggcggcgca gcattgtgcg cgactttttc ttcacgctga
     4981 aaactctgcc gcggcatgtc tggaagctgc acccgaacac cgtgttcacg ctggtactga
     5041 cacccttggg cgcgctggtg gggcttctgg tggtgttgac cgcgctcact accgatccgc
     5101 tggcctgggc cggacctgta ccgctggtca gcgcgctggc ggtgggtgcg gtgctgacgt
     5161 gggtgatcaa gaaatacagc gcggaagagg cggtcacgca tccgctggcc tttggcgcat
     5221 atgtagcgtg gac
//
LOCUS       DASPUL010000264         2305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_186683, whole genome shotgun sequence.
ACCESSION   DASPUL010000264 DASPUL010000000
VERSION     DASPUL010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186683"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VEJ00_06560"
     CDS             complement(<1..303)
                     /locus_tag="VEJ00_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00857.1"
                     /translation="MRLSYSSLRNGSRRLLWMMVFLGCAAGLAEGTDVVTFHNDNLRS
                     GLNAQETILTLSNVNSSSFGKLFTLAVDGVVDAEPLYLAGVSFPGQGVHNGLYVVTE"
     gene            complement(449..673)
                     /locus_tag="VEJ00_06565"
     CDS             complement(449..673)
                     /locus_tag="VEJ00_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00858.1"
                     /translation="MLQEMVGSQFLVALAHSYEEDPRRFVSLPKPMLDSSVARMAVAE
                     LRNEGYVEEQVRGVIRLTPRGYKAYKEHIN"
     gene            complement(870..>2305)
                     /locus_tag="VEJ00_06570"
     CDS             complement(870..>2305)
                     /locus_tag="VEJ00_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00859.1"
                     /translation="KVGYNSVQAATNGIEFAKSFEFAQQAVEAGMRYAYLQFDGIGNA
                     ANAHRKVGNLFDVKLKAIDNLHAAGVEIVPVVTIVNGINNEQVGRIIQFALDNPKKIG
                     FLSFQPVSFTGRDEAITDERRVAQRYTLAHLAHDVKNQTGLGEPARDWFPISFMGTFS
                     DWADLMHAQDGNANWGQLSCGCHPNCGTGMAVMIDKETKESVPVTAFLHGDQLAKDIA
                     KVNDAARGRFLSIFGMALCLMKNYDPFQSPTHFKLIDLLRKFDKNYNATGHNYGKVGP
                     DRTIDDVMKRRQDRWNFLFIAGMWFQDLFNYDFRRTEQCIIPYATQEGEISFCAYNTG
                     VGWRNIIEKMHMTATLTKWYEEHGRHEIFAGGKNVKMENTEHSLVLREGIVTEEEQHD
                     LDKLGIAKNAREEKIRARDAKQAEAAARGPKKNDAAYDARMAQLYREVVLKEKPAHSE
                     NGFIPLGALAKVNAPKPTEVGEPVAGD"
BASE COUNT          440 a          719 c          635 g          511 t
ORIGIN      
        1 ctccgtgacc acatacagcc cattgtgcac gccctgcccg gggaaagaca ctccagccag
       61 atacagaggc tcggcgtcaa cgacgccatc cactgccagg gtaaacagct tgccgaaact
      121 ggaggagttg acgttgctca gcgtcaaaat cgtttcctgc gcgttgaggc ccgaccgcag
      181 attgtcgttg tgaaaagtta cgacgtccgt cccctccgcc aggccggctg cgcagcccag
      241 gaacaccatc atccacagca acctgcggct gccgtttctc aggcttgaat aagaaagtct
      301 catgcccaac ctgccctttg ccttaaacgt acacgtatcc ggatcttggt tcacaggaac
      361 cgcgttcgag gggggatgaa gagacggaca agcccgatca gcgggcctta ttgtaccatg
      421 ctatttaaag tgagtgcccg aggcagggct agttgatgtg ttccttgtag gccttatatc
      481 cgcgcggcgt cagacgtata actccccgca cctgctcctc gacatagcct tcattgcgca
      541 gttcggcaac cgccatgcgc gccaccgagg aatctaacat cggcttcggc aagctgacga
      601 accggcgcgg gtcctcctca taggagtggg ccagcgccac caggaactga ctgccgacca
      661 tttcctgcag catacctatc tcctcatctg cacgttaatg ctacgcggtc ctcagcgggc
      721 tcggaatggc tcacctgtgc cagccagtgg caccttggtc acgaacccaa gttgttgaaa
      781 cgcaaagata tatttaaaca acccaaaaac ttgggccgcc tttcggcggc cccactgaga
      841 actgagaacc gggaactgag aactgctttc taatctcccg ctactggctc cccgacttcc
      901 gtcggttttg gggcatttac cttggccagc gcgcccagcg ggataaagcc attctccgag
      961 tgggctggct tctccttcag cacgacttca cgatagagct gtgccatacg ggcgtcgtag
     1021 gccgcgtcat tcttcttcgg accacgcgcg gcagcttcag cctgcttcgc atcccgcgcc
     1081 cgaatcttct cctcgcgcgc gttcttggcg atccccagct tgtccagatc atgctgctct
     1141 tcctcggtca cgatcccttc acgcagcacc agcgagtgtt ccgtgttttc catcttcacg
     1201 ttcttgccgc cggcgaagat ctcgtgccgc ccgtgctctt cgtaccactt ggtgagcgtg
     1261 gcggtcatat gcatcttctc gatgatgttt cgccagccca cgcccgtgtt gtacgcgcag
     1321 aaggaaatct ctccctcctg cgtcgcatag ggaataatgc actgctcggt gcgccggaag
     1381 tcgtagttga acagatcctg gaaccacatc ccggcaatga acaggaagtt ccagcgatcc
     1441 tgccgccgtt tcatgacatc gtcgatcgtg cgatccggtc cgactttgcc atagttgtgc
     1501 ccggtcgcgt tgtagttctt gtcgaacttg cgcagcaggt cgatcagctt gaaatgggtc
     1561 ggcgactgga acggatcgta gttcttcatc aggcacagcg ccatcccgaa aatactcaga
     1621 aatctcccgc gcgccgcatc gttcaccttg gcaatatctt tcgccagctg gtctccgtgc
     1681 aggaacgccg tcaccggcac gctctccttg gtttctttgt cgatcatcac cgccatcccc
     1741 gtcccacaat tcggatggca gccgcaggag agttggcccc agttggcgtt gccatcctgg
     1801 gcgtgcatga ggtcggccca gtcggagaag gttcccatga agctgatggg gaaccagtcg
     1861 cgggcgggct cgccgaggcc ggtctggttc ttcacgtcgt gggcgaggtg agcgagcgtg
     1921 tagcgctgcg caacacggcg ctcgtcggtg atggcctcgt cgcggccggt gaacgagacc
     1981 ggctggaacg acaggaagcc gatctttttg ggattatcga gcgcgaactg gatgatgcgt
     2041 cccacctgct cgttgttaat tccgttgaca atcgtcacca ccggcacgat ttctacgccg
     2101 gcggcgtgca ggttgtcgat ggccttgagc ttcacgtcaa acagattgcc gaccttgcga
     2161 tgagcgttgg cggcgttgcc gatgccgtca aactgcagat aggcgtagcg catgccggct
     2221 tcgaccgcct gctgggcgaa ctcaaagctc ttggcgaact cgatgccgtt ggttgctgcc
     2281 tgcaccgagt tgtatccaac cttgc
//
LOCUS       DASPUL010000265         5877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1822955, whole genome shotgun sequence.
ACCESSION   DASPUL010000265 DASPUL010000000
VERSION     DASPUL010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822955"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="VEJ00_06575"
     CDS             complement(<1..1503)
                     /locus_tag="VEJ00_06575"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00860.1"
                     /translation="MMATSPLAGELRSSLTDESARMQRAFEASGDGRAAVAQRTRLIE
                     DILQRLWREIVTSGEPCLDGPSGFSLVATGGFGRGWLFPYSDIDLLFLFADRDAEQKY
                     KDAVRRFSQELWDLKLKLSPASRTFAECDRYDANNTEFTISLLDCRYLAGHRELFSRL
                     HDKLVPKLVMREAKPLVQGLAEVTRERHGKHGLTLYHLEPNLKEAPGGLRDYNVACWL
                     ALISAIDKLHDWPHASSLRAPVRKQLEAALDFLMAARCFLHFRHGRDDNTLSWEAQDE
                     AAARKIGVSDAGELSAADWMRIYFGHARAVQRTVTQLLEEIPEAWSALYRQVQSWRSR
                     LTTADFSVVDGLIFLQQPAALQDPEVLLRLFHFMAHHGLKLSATTEYRIEQALPALAA
                     TPPRGAELWLYLQETLLQPYAADALRAMHSLRLLTLLLPELKLIDSLVVRDFYHRFTV
                     DEHSFLAIESLHRLKQSQSEWDKRYGGLLGELEDPELLYLALLLHDTGKGV"
     gene            complement(1637..1975)
                     /locus_tag="VEJ00_06580"
     CDS             complement(1637..1975)
                     /locus_tag="VEJ00_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXX00861.1"
                     /translation="MTKLEAIIQNSKLDAVKAALHEIGVEGMTVIEVRGHGRQKGHTE
                     VYRGREYSVDLIPKTKLEMVLPDAMVEKAEQAILNAARTGKIGDGKIFLSRVDEAIRI
                     RNDERGEGAL"
     gene            complement(2072..3544)
                     /locus_tag="VEJ00_06585"
     CDS             complement(2072..3544)
                     /locus_tag="VEJ00_06585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HXX00862.1"
                     /translation="MKVRSRESHDWSHAEVPIAKRLIIRKVTALFILTFFLLTLGQVV
                     CAQTAADTNARLAKLEQQAADAKSSADNAWMLTSAALVLMMTGPGLALFYGGLVRKKN
                     VLGTMMQSFALMAVVTVLWGLVSYSLAFGSGNSFVGGVHNLFLRGVGAAPDADYAATI
                     PAQTFMVYQLMFAIITPGLIAGAFAERMKFSAMLVFMILWTFLVYDPMAHMVWGKGGL
                     LNASLGGRFPTLDFAGGTVVHITSGVSALVCALYLGKRVGFPKEPMPPHSVVLSFIGA
                     CLLWVGWFGFNAGSALAAGSLATSAFVATHFAAAAAALGWAGAEWVRNGKPSVLGGIS
                     GSVAGLVAITPAAGFVKPMPALAIGLIAGVFCYWMVARVKAKFGYDDSLDAFGVHGAG
                     GTLGALLTGVFAVSAVNPIFKDAQGNTLASGLFEGNGRQLLNQAVGVAIAWGLAIVGT
                     LLILKIVDITIGLRVTEEEEVQGLDLSQHGEEGYYWEASA"
     gene            complement(3656..4888)
                     /locus_tag="VEJ00_06590"
     CDS             complement(3656..4888)
                     /locus_tag="VEJ00_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00863.1"
                     /translation="MRPYFTRERFGQPQFLAGLLLLAFLMQGVWLVHAELRGGTEPEG
                     PEAERIGEGWKQWQGRGIAGAPRVEGPGDSPEPFRNRNDDGFDREHSPLLYLVTAAPL
                     LVCSHTLYPETAYWRWLPRLPFLLIGVSLGASLWYVARRLCGNTGGFAALTFYCFSPA
                     FIQAAAVWHTEPEILAAWGSFGTIFTAIAVAHTLYAPREVVLWNWRRIVLLGVSLAIS
                     VGSQFSMIVLVPMALGFLVYVAPVRRGAGFVIWLAACAVGLVLLFAVYFFQPHAFAES
                     MRRAEFWGATWRAFAIWGVYKQVAVEIGRACPALVLALPVAVASYAAWPRARYFGNTA
                     PLVVGVSFLILGVAHPHSGGAGFLLAAIPFLFIFVSGVLADLMETRHRSLVTACILGM
                     MAAYLASSLLGLAHVGRG"
     gene            4999..>5877
                     /locus_tag="VEJ00_06595"
     CDS             4999..>5877
                     /locus_tag="VEJ00_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HXX00864.1"
                     /translation="MMPDRSNDAPAPSSPETPPPNFSATDQPSYARTLFLGPGGLRPG
                     WGLAFYFAMYYPLKRILLEFAYATDLGSAGLWNQMLGEFVSLLAALIPALILARVERR
                     PWRAYGLPGRQFLGKLFWVGLLWGFAAISLLIFALYGVHAFHFGHVVLHGARLARFAA
                     FWALMFLLVGLFEEFLTRGYAQFTLARGIGFWPAAVVLSVVFAAMHLPNDGETPSGLA
                     AVVCIGFFFCLTLRRTGNLWFAVGFHAAWDWGQTYFYSVPDSGLVAPGHLLSSSLRGP
                     SWLSGGSVGPEGSALCF"
BASE COUNT         1193 a         2008 c         1632 g         1044 t
ORIGIN      
        1 cacgcccttg cccgtgtcgt gcagcagcaa cgccaggtac aacaactcgg gatcctccag
       61 ttcgccgagc aacccgccat accgcttgtc ccactccgat tgcgattgct tcaatcggtg
      121 cagactctcg atcgccaaga acgaatgttc gtcaacggta aatcgatgat aaaaatcgcg
      181 taccaccagc gaatcaatga gcttcaattc cggcagcagc aacgtgagca gccgcaggga
      241 atgcatcgcc cgcagcgcgt cggcggcata gggctgcagc aaagtctcct gcagatagag
      301 ccacaactcc gctccccgcg gcggcgtggc ggccagcgcc ggaagcgcct gctcgatgcg
      361 gtactccgta gtggcgctca gcttcagccc gtgatgcgcc atgaaatgaa acaaccgcag
      421 caggacctcg ggatcctgca gcgctgccgg ctgctgcagg aaaatcagcc cgtcgaccac
      481 cgaaaaatcc gccgtcgtga gccgcgaccg ccagctctgc acttgccggt agagcgccga
      541 ccaagcctcc ggaatttcct ccagaagctg cgtcacggtc cgctgcacgg cgcgtgcgtg
      601 gccaaagtag atccgcatcc agtcggctgc cgaaagctcg cccgcatccg acactccaat
      661 cttcctcgcc gccgcctcgt cctgcgcctc ccagctcagc gtgttgtcgt cgcgcccatg
      721 ccggaagtgc aaaaagcagc gcgcggccat cagaaaatcc agtgccgcct cgagttgctt
      781 gcgcacagga gcccgcaacg aactcgcatg cggccagtcg tgcagtttgt cgatcgccga
      841 aatcagcgcc agccagcagg ctacgttgta gtccctcaga ccgccgggag cttccttcag
      901 atttggctcc aggtgataga gggtcagacc atgcttgccg tgccgctcac gtgtgacctc
      961 cgccagccct tgcaccagcg gcttggcttc gcgcatgacc agcttcggga ccagcttgtc
     1021 gtgcagccgc gaaaacagct cgcgatggcc agcgagatag cggcagtcaa gtagagagat
     1081 cgtgaactcg gtgttattgg cgtcgtagcg gtcgcactcc gcgaatgtcc gcgacgccgg
     1141 actcagcttc agcttcaaat cccacagttc ctgcgagaac cggcgcacgg catctttgta
     1201 tttttgttcg gcgtcgcggt cggcgaaaag gaagaggaga tcgatatcgg agtagggaaa
     1261 cagccacccc cgcccgaatc cacctgtagc tacaagggaa aaaccagagg ggccgtcgag
     1321 acagggttcg ccagaggtaa caatctctcg ccacaaccgc tgcaggatgt cttcgatcag
     1381 ccgtgtacgc tgcgccacag ccgcgcgccc gtcaccgctg gcctcaaagg cccgctgcat
     1441 gcgcgccgat tcgtctgtga gggaactgcg aagctcgccg gccaatgggg acgtcgccat
     1501 catagattta gctgccagca gtccctggga gggcaggttt tagctgtacc tatgagggcg
     1561 tcatcccgaa gccccgcgct ttcaccagcg gggcgaggga tctcgcttgg agcgaaagcg
     1621 gacgacacgc aggaagctac agcgcccctt ccccccgctc atcgttgcgg atgcggatcg
     1681 cctcgtccac gcgcgagaga aaaatcttgc catcaccgat ctttcccgtg cgtgccgcgt
     1741 tcagaatcgc ctgctcggcc ttctcgacca tcgcatccgg aagcaccatc tcgagcttgg
     1801 ttttgggaat caggtccacg ctgtactcgc gaccgcgata gacctcggtg tggcccttct
     1861 gccggccgtg cccgcggacc tcgatcacgg tcatgccttc gacgccaatt tcgtgcagcg
     1921 cggccttcac cgcgtccagc ttggagtttt gaatgatcgc ctcaagcttc gtcataactc
     1981 tcaaatccct cgtgtggcgc gggcactcct gcccgcgaac gctagcgtca tggccttggc
     2041 agcgcggaca aaattgcccg cgccacacct cttacgccga cgcctcccaa taatatccct
     2101 cttcgccgtg ttgggagaga tccaggccct gcacttcctc ctcctcagtg actcgcaacc
     2161 cgatcgtgat gtcgacaatc ttgaggatca gcagcgtgcc cacgatcgcg agcccccacg
     2221 caatggccac gccgaccgct tgattgagca gttgacgccc attgccctca aacagccccg
     2281 acgccagcgt atttccctgc gcatccttga agatgggatt gaccgcgctc accgcgaaca
     2341 cccccgtcag cagggcgccc agcgtcccac cggccccgtg cactccgaag gcgtcgagcg
     2401 aatcgtcata cccgaacttc gccttgaccc tggccaccat ccagtagcag aagacgccgg
     2461 cgatcaggcc gatcgcgagc gcgggcatcg gcttcacgaa tccggccgcc ggcgtaatcg
     2521 ccaccagtcc cgcgaccgat cccgagattc cgcccagcac actcggcttg ccgttccgca
     2581 cccactccgc tccggcccat cccagcgcgg cggccgcagc tgcgaagtgt gtcgccacaa
     2641 acgcgctagt cgcaagacta cccgccgcca gcgcgctccc tgcgttgaag ccaaaccagc
     2701 ccacccatag caagcacgcg ccaatgaaac tcaacaccac gctgtgcgga ggcatcggct
     2761 ccttcgggaa tcccacgcgc ttgcccagat acaacgcgca caccagcgcc gatactcctg
     2821 aggtgatgtg cactaccgtc ccgccggcaa aatcgagcgt ggggaaccgc ccgcccagag
     2881 aggcattcag cagcccgccc ttgccccaca ccatgtgtgc catcgggtca taaacaagga
     2941 aagtccagag aatcatgaac accagcatgg cgctgaactt catgcgctcg gcaaacgcgc
     3001 ccgcgatcag tcccggcgtg atgatcgcga acatcaactg gtagaccatg aacgtctgtg
     3061 cgggaatcgt tgccgcatag tccgcatccg gcgccgcgcc cacgccccga agaaaaagat
     3121 tgtgcacccc acccacaaag ctgttgcccg agccaaacgc caagctgtag ctgacaagcc
     3181 cccaaagcac ggtgaccacc gccatcagcg caaaactttg catcatcgtg ccgagaacgt
     3241 ttttcttgcg caccagcccg ccgtagaaca gcgccagccc cggaccggtc atcatcaaaa
     3301 ccagagccgc gctggtcagc atccaggcgt tgtcggcgga actcttcgca tcggccgcct
     3361 gctgctccag cttggccagc cgcgcattcg tgtccgcggc cgtctgcgcg cagactacct
     3421 gtccgagggt gagaagaaag aatgtcagga tgaataaggc cgtcaccttg cggatgatca
     3481 gccttttcgc gattggcact tcagcatgac tccagtcgtg ggattcgcgc gatcgaacct
     3541 tcactctatc gtccgcctcc gcttcgcggg aaatcgaaac tatttatcat ggctatcagg
     3601 ttttgttatt gaacgggctg tcaggctagt gcgacgaaga aagagaaact cccttctaac
     3661 ctcttccaac gtgcgccagg ccgagtaggc tcgacgcgag ataggcagcc atcatcccga
     3721 gaatgcacgc cgtcaccagc gaacgatgac gcgtctccat gagatccgcc agaacaccag
     3781 agacgaaaat gaagagaaaa ggaatcgctg ccagcaggaa cccggctccc cccgagtgag
     3841 gatgggcgac tccgagaatc aggaacgaca cacctaccac taacggcgcg gtgttcccga
     3901 agtagcgcgc gcgcggccac gctgcatagc tcgcaacggc aaccggaagc gcgagcacga
     3961 gcgccggaca cgcccggcca atctccaccg cgacctgctt gtaaactccc cagatggcaa
     4021 acgcgcgcca ggtagctccc caaaattccg cgcgccgcat gctttcggcg aaagcatgtg
     4081 gctggaagaa atagacggca aacagcagga ccagtcctac cgcgcacgcc gccagccaga
     4141 tcacgaaccc cgctccgcgc cgcaccggcg cgacatagac caagaaaccc aacgccatcg
     4201 gtacaagcac gatcatcgag aactgcgatc ccacggagat ggccagcgag actccaagca
     4261 acacgatccg ccgccagttc cacaggacga cttcacgcgg cgcatagagt gtgtgtgcca
     4321 ccgcgatcgc agtgaagatc gtgccaaatg atccccacgc tgccaaaatc tccggctccg
     4381 tatgccagac cgcagccgcc tgaatgaacg ccggcgagaa acaataaaac gtcagcgcgg
     4441 caaagccccc ggtattgccg cacaggcgcc gagcgacgta ccagagcgaa gcaccgagag
     4501 agacgccgat aagcagaaaa ggaagcctgg gcagccagcg ccagtacgcc gtctccgggt
     4561 acagagtgtg ggagcagact agcaacggcg ccgccgtcac cagatacagc aacggcgaat
     4621 gctcgcggtc gaagccgtcg tcgtttctgt tgcggaaggg ttcgggtgaa tcgcccggac
     4681 cttcaacccg tggtgcacct gcaatgccac gcccctgcca ctgcttccag ccttcgccaa
     4741 ttcgttccgc ttccggtccc tccggctctg tccccccgcg aagttctgca tgcaccagcc
     4801 agaccccctg catcagaaat gcaagcagca gcaatccggc aagaaactgc ggttggccga
     4861 atcgttcgcg cgtgaaatat ggacgcatac ggtcgagccg agtgttttac catgtcgagg
     4921 gccatcgcgg ggaacttctt cgaagcggcc gctggtcttt atgagaactg actcctggct
     4981 cttatccttg gctcgtgaat gatgcccgat cgctccaacg acgcacccgc cccgtcttcc
     5041 ccggaaacgc ccccgccgaa cttttctgca acggatcagc cttcctacgc gcgcaccctg
     5101 tttctcggcc ccggtgggct gcggcccggt tgggggctcg ccttttattt cgccatgtac
     5161 tacccgctga agcgaatcct gctggaattc gcatacgcga ccgacctggg ctcagcaggc
     5221 ctgtggaacc agatgctggg agaattcgtc tccctgctgg cggcactgat tccggcgttg
     5281 attctcgcgc gagtcgaacg ccgcccctgg cgggcctacg gactgccggg gagacagttc
     5341 ctgggaaagt tgttttgggt aggactgctg tggggattcg ctgccatcag cctgctgatc
     5401 ttcgcactgt acggcgtgca cgcgtttcac ttcgggcatg tcgtcctgca cggagcgcgt
     5461 ctcgcccgct tcgctgcctt ctgggcgctg atgttcctgc ttgtcggcct cttcgaagaa
     5521 tttctcaccc gcggctacgc acaatttacc ctggcccgtg gcatcggatt ctggcccgcg
     5581 gccgtcgttc tatccgtcgt ctttgccgcc atgcatcttc ctaacgacgg cgagactcct
     5641 tcaggattgg cggcggtggt ctgcatcggt tttttcttct gcctgacgct gcggcgcacc
     5701 ggcaacctgt ggttcgccgt gggattccac gccgcctggg attggggcca gacgtatttc
     5761 tactctgtcc ccgacagcgg cctggtcgca cccggtcacc tcctcagctc atccctgcgt
     5821 ggcccgtcgt ggctcagcgg aggctccgtc ggcccagaag gcagcgcgct gtgcttc
//
LOCUS       DASPUL010000266         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_453822, whole genome shotgun sequence.
ACCESSION   DASPUL010000266 DASPUL010000000
VERSION     DASPUL010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453822"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="VEJ00_06600"
     CDS             complement(<1..1181)
                     /locus_tag="VEJ00_06600"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HXX00865.1"
                     /translation="MRAFRLLLAFVLAAAVGSFSQTASSTRPSARFSIDNIDKSLDPC
                     VDFYQFACGNWLKRSEIPADQSTWVSFVELNESNRDIEHTILEKAAAGGASRDPIDQK
                     IGDLYGACMDEKTVNAKGVSAIQPELDRVTAAQDKAALIDEIGHIHLLGPNPLFNFYS
                     QSDLHNADQVIAFIDQGGLTLPDRDYYLKNDPDHTAKRQRLVDYVKEVFALAGQTPQQ
                     AADSAQTVLRIETMLAFDSMDRTQRRDPKNRDHKMSRDEAVALGSNFYLDRYFKAVGA
                     PDFTQLNVVNPDFFKQVNGVLQAESLDALKTYVSWHVLNAAAPWLSQPFVDANFKLQH
                     DLTGQKEIQARWKRCVGLTDRELGEALGKKYVDVTFPPDSKARMLKMVDALEKSLDAD
                     I"
     gene            complement(1375..>2135)
                     /locus_tag="VEJ00_06605"
     CDS             complement(1375..>2135)
                     /locus_tag="VEJ00_06605"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HXX00866.1"
                     /translation="FRILAALGLSFAKSRLAESFWREFMARYGTLGDYRFSDTQEAAL
                     DIRGAMVYGPTDQKLGKIDDVIFDEATGAITFVVIDTGGWLSSKRFIVAPSEIRPSLQ
                     HGDDFLVDLTKEQIESFPPYDSGDLSSAEKWADYERRYRSKWVEKPVMHREATDRNIT
                     PTTKQQMGAGSGTIPASEADTAELDIETELTAVHPEADMDVDPAGPTLRWSAFEGTLR
                     RRREEVPESSIDNLKKTPGEKPSASERIERRKAS"
BASE COUNT          421 a          575 c          678 g          461 t
ORIGIN      
        1 ttgatgtctg cgtccaggga tttctcgagc gcatcgacca tcttcaacat gcgggctttg
       61 ctgtcgggtg ggaaagtgac gtcaacgtac ttcttgccca gcgcttcgcc aagctcacgg
      121 tcggtaagcc cgacgcagcg cttccagcgc gcctgaattt ctttttgacc ggtgaggtca
      181 tgctgcaact taaagttggc gtcgacgaaa ggctgggaca gccagggagc ggcggcgttg
      241 agaacgtgcc agctgacgta ggttttgaga gcgtcgagcg actcggcctg caacacaccg
      301 ttcacctgtt tgaagaagtc tgggttgacg acgttgagct gggtgaagtc gggcgccccg
      361 accgccttga agtagcgatc aagatagaag ttcgacccga gagcgacggc ttcgtcgcgc
      421 gacatcttgt ggtcgcggtt cttgggatcg cggcgctggg tgcgatccat ggagtcgaag
      481 gccagcatgg tttcgatgcg caggacggtc tgggccgagt cggcggcctg ttgcggagtt
      541 tgtccggcca gcgcgaacac ctccttcacg tagtcgacca ggcgctggcg tttggcggtg
      601 tggtcgggat cgttcttcag gtagtagtcg cggtcgggca gggtgaggcc accctggtcg
      661 ataaatgcga tcacctggtc ggcgttgtgc aggtcggact gggaataaaa attgaagagc
      721 ggattcggac caagcaagtg gatgtggccg atctcgtcga tcagcgcggc tttgtcctgg
      781 gcggctgtaa cgcgatcgag ctcgggctgg atggcagaga ctcctttggc gttgactgtc
      841 ttctcatcca tgcacgcgcc gtagagatcg ccaatcttct gatcaatggg atcgcgactc
      901 gcaccgccgg cggcggcctt ctccagaatg gtgtgttcaa tgtcgcgatt gctttcgttc
      961 aactcgacga aactgaccca ggtcgactgg tctgcgggaa tctcgctgcg cttgagccag
     1021 ttgccgcagg caaactggta gaagtcgacg caagggtcca ggctcttgtc gatgttgtcg
     1081 atgctgaagc gggcactggg ccgggtagag gaagcagtct gcgaaaagct gccaacggcc
     1141 gcggccagca cgaaggccag cagaagacga aacgcacgca tgagaatttc tccttaggaa
     1201 atgcgtcgaa ggatgatggg caacgggtga ttctatgccc ggtgcgccgg atgagcaaca
     1261 agatcagcga ggcgaagaga cataacaaat tgtgagggct tggcgcggaa gctctggtgt
     1321 gcgctcccga aaattgggtt ccgggagcgc acacgtcgct ctacgagtac gtgcttagct
     1381 ggccttgcgc cgttcgatgc gctcgctcgc ggaaggcttc tcgcccggag tttttttcaa
     1441 gttgtcgatg gaagactccg ggacctcctc gcggcgccgg cgcaacgtcc cttcgaaagc
     1501 gctccagcga agcgtaggac cggcgggatc gacatccata tcagcttcgg gatgaaccgc
     1561 cgtcagttct gtctcaatgt ccagttcggc tgtatccgct tccgatgcgg gaatcgtgcc
     1621 cgatccggcg cccatttgct gcttggtggt gggagtgatg ttgcgatcgg tggcttcacg
     1681 gtgcatgacc ggcttctcta cccatttcga gcgatagcgg cgttcgtagt cggcccactt
     1741 ctcggcggaa gaaagatcgc cgctgtcata gggcggaaag ctctcgatct gctccttggt
     1801 aagatcaacc aggaaatcgt ccccatgctg cagcgaagga cggatttcag agggcgccac
     1861 gatgaatcgc ttgctggaga gccagcctcc cgtgtcgatg accacaaacg tgatcgcgcc
     1921 ggttgcctca tcgaaaatca cgtcatcgat cttgcccagc ttttgatctg tgggcccata
     1981 gaccatggcg ccgcgaatgt cgagcgctgc ttcttgcgta tcggaaaagc gataatcccc
     2041 taaagtgccg taacgcgcca taaattccct ccagaatgat tctgcaagcc gggatttcgc
     2101 gaatgacaat cccagtgcgg ctaagatgcg gaaat
//
LOCUS       DASPUL010000267         5210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1690811, whole genome shotgun sequence.
ACCESSION   DASPUL010000267 DASPUL010000000
VERSION     DASPUL010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690811"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VEJ00_06610"
     CDS             complement(<1..677)
                     /locus_tag="VEJ00_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00867.1"
                     /translation="MFDAGRPRGRLSCWVLLPALALGFQLSAFGEAAAQGAPATTTVT
                     DIVYRADGSTAQGNLIIFWPAFVTAGGAAVAAGTTNVTLAANGSFSVGLVSNAGATPA
                     GVYYTVVYQLGPGEVKTEYWVVPTTSPANLATVRTTPGGSVATPPVSMQYVNSQLATV
                     VHLGGTETITGAKTFTVTPSVPTPTSAGEVANKAYVDQSLSNVGAGNYLPTAGGTMTG
                     PITLPANP"
     gene            complement(793..1251)
                     /locus_tag="VEJ00_06615"
     CDS             complement(793..1251)
                     /locus_tag="VEJ00_06615"
                     /EC_number="3.1.3.16"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase"
                     /protein_id="HXX00868.1"
                     /translation="MDITWVTDRIAVGGGIWTSDNMAKVAQAGITHIIDMQIEFDDTP
                     LALDHGVAVLWNPTDDDFELKPAALFRRGVDFAEAALSSDGAKLFVHCAAGVHRAPMM
                     TLAILGSMGWKVDDAMQLIEARRPVADFAGVYVQSVENFLDGRDRGSQRE"
     gene            complement(1559..2002)
                     /locus_tag="VEJ00_06620"
     CDS             complement(1559..2002)
                     /locus_tag="VEJ00_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00869.1"
                     /translation="MIKKKTKKKSTAKAPARKRPAQKKQTDPAQVREEIAAMVKSGAK
                     DITEAVLDQAMHGELAPAKYLFEVAQIYPPANDGESATEEEDCLAKTLLARIDAGRKP
                     ASTEEVDEDDKAEDEATDERNIFNGDKTAGRDEARDDGAAPDGSE"
     gene            complement(2299..2970)
                     /locus_tag="VEJ00_06625"
     CDS             complement(2299..2970)
                     /locus_tag="VEJ00_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00870.1"
                     /translation="MSGMIAWIAASAEKRTVPPGEEIRWRRREKRREQEYADEILLPG
                     LPVHQFPAVNSLPAKNAGDGQPEVGAELDPEHERERRMYRGRTVAMLRRYMRYSLETG
                     RMPSLLGREFFRATVTKYRVTTFEDRVIFVHDMEICLEQLDEFSRQLIARHILQEHDQ
                     AATSRLLQCSERTVRTYVPVVLDLLAEILLEVGLLDKRISTGKNSCQEGESGRIAASD
                     WEDGK"
     gene            complement(2967..3239)
                     /locus_tag="VEJ00_06630"
     CDS             complement(2967..3239)
                     /locus_tag="VEJ00_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00871.1"
                     /translation="MKSNRRDGDDEIVRMELKYCEHCGGLWLRECGAGVVYCENCQPR
                     IADLPIPKKKPARVSLPVQRRSLVGNLPFKVDDTDDLDFEAAGGAA"
     gene            complement(3502..3744)
                     /locus_tag="VEJ00_06635"
     CDS             complement(3502..3744)
                     /locus_tag="VEJ00_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00872.1"
                     /translation="MSTHADNSSEERDFGEFRRKVRSAEVLSASMERLLLSLRFTGRV
                     SVVMQNGRVLKSGYEEGYFRQRVEGGGADDEIAHKL"
     gene            3897..4613
                     /locus_tag="VEJ00_06640"
     CDS             3897..4613
                     /locus_tag="VEJ00_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00873.1"
                     /translation="MKFKVLQDNLRKILRDRIDAGELTGLRLARLTRFKQAHISNFLN
                     KKRGLSLEGMDKVLSVQHLSVLDLLDQAEVNQRASILPPSGDKFENVILADAATAATR
                     RSITARQVKEILKFKKSFLQRLKPKTVGNRSGWQRFVLIKVDPADARAMHPRLIPGAV
                     LLLDRHYNSLRRYRTGALNMYAVLKNDTCAIRYVEAAESCLILRPHNPHAPIEVIPVE
                     DGKSAEDYIVGRVCHAGLEM"
BASE COUNT          949 a         1784 c         1326 g         1151 t
ORIGIN      
        1 ggatttgccg gcaaagtgat cggccccgtc atggtgcctc cggcagtcgg cagataattg
       61 cccgcaccca cattcgacag cgattgatcc acatatgcct tgttcgccac ctcccccgcg
      121 ctcgtcggcg ttgggacgct cggcgtgacc gtgaatgtct tcgcccccgt gatcgtctcc
      181 gttcctccaa ggtgcacgac cgtcgccagt tgcgaattca catactgcat cgaaaccggg
      241 ggcgtggcca cgctccctcc cggcgtggtc cgcaccgtgg ccaggtttgc cggagacgtc
      301 gtcggcacca cccagtactc cgtcttcacc tcgcctggcc ccagttggta gacgacggtg
      361 taatacacgc ccgccggagt cgctcccgca ttcgacacca gccccacgct aaacgatccg
      421 ttcgcagcca gcgtcacgtt cgtcgttccc gccgctaccg ccgccccgcc tgcggtcaca
      481 aatgccggcc agaagatgat caggtttcct tgcgctgtgc tcccatctgc ccggtacaca
      541 atgtctgtga ccgtagttgt cgcgggcgcg ccctgtgcag ccgcctctcc gaaagcggaa
      601 agctggaagc ccagcgccag cgccggtagc agcacccaac aagaaagccg ccccctgggg
      661 cggcctgcgt caaacatgat ctcctccatc taactcgctg cggcggttgc cttcgtggga
      721 cttcgtaccc ttcgtggtcg ggtgccccat ttctcgcgcg tttttggcgc gagcagtggg
      781 gcttttccgc gtctattccc tctgcgatcc gcgatcccgc ccgtccagaa aattctccac
      841 gctctgcacg tacacgcccg caaaatccgc caccggacgc cgcgcctcga tcagctgcat
      901 cgcgtcgtcc accttccacc ccatcgatcc cagaatggcc agcgtcatca tcggtgcgcg
      961 gtgcactccc gcggcgcaat gcacaaacag tttcgcgccg tcgctcgaaa gcgcagcctc
     1021 ggcaaaatcc actccgcgcc gaaacagagc ggccggcttc agctcaaaat cgtcatccgt
     1081 cggattccac agcaccgcaa cgccatgatc gagtgccaat ggcgtgtcgt cgaactcgat
     1141 ctgcatgtcg atgatgtgcg tgatccccgc ctgcgccacc ttcgccatgt tgtcggatgt
     1201 ccagatcccc cctcccaccg cgatccggtc cgtcacccag gtaatatcca ttcccgtcct
     1261 gtgtggggca ggtgggtgcc ccatcctaag cgcgtctttt gcgcttaggg tggggatttt
     1321 gactcgctct ttcagggttg ggtgccccat ttctcgcgcg ttctttgcgc gagaagtggg
     1381 gattgtgacc ttgcgtctct ctccaaccca gactgccaga gctcgttctc gctgcactac
     1441 cccgcctcat tctacggtat tgccgaaccc ggattctgtg gagcaggaat caccgtctcg
     1501 ctcgcgaccg gatcgggctt tcctccgcgt gcttcgcgag ctctgcagtc aaaagctctc
     1561 actcgctccc atccggagcc gcgccgtcgt ccctggcttc atcgcggcct gccgtcttgt
     1621 cgccgttgaa aatgtttcgt tcgtccgtgg cctcatcttc agctttgtcg tcctcgtcga
     1681 cttcctccgt ggacgctggc ttcctccccg cgtcaatccg ggccagcaag gttttggcca
     1741 ggcagtcttc ctcttctgtg gccgactcgc cgtcgttcgc cggcggataa atctgcgcca
     1801 cctcgaacag atacttcgcc ggtgccagct cgccgtgcat cgcctggtcc aatactgcct
     1861 cggtgatgtc cttcgccccc gacttcacca tcgcggcaat ctcctcccgc acctgcgccg
     1921 gatccgtctg cttcttctgc gccggccttt tcctcgcggg ggccttcgct gtactcttct
     1981 tcttcgtctt tttcttgatc atcgccccac tgcccgaatg tccctggact ctccaccaaa
     2041 ccgcccatcc ctttcccccc aaagcacgct tgtcatcctg agcgaagcgc ggtttgctga
     2101 gcgaaggatc taggcgggtg acggcgcgtc atcccgccca gcgggatgac atttaagcgc
     2161 gacgcgcgca tcctctcccc aaaaaaccga gggcgcgctc ttcagcgcgc cctcttttca
     2221 aggagaatct cgtttttctc actctctaaa ttcagaatag caaatggcta agggcagacc
     2281 ggcaaagttt tgaaattttt atttaccatc ttcccaatca cttgcggcga ttcgaccgct
     2341 ctccccctct tgacaagaat ttttccccgt cgagatcctc ttgtccagaa gccccacttc
     2401 gagcaagatc tcggccaaca gatccaggac aacgggaaca tatgtccgta cggttctctc
     2461 actgcattgc agaagcctgc tggtcgcggc ctggtcatgc tcctgcaaaa tgtgccgcgc
     2521 gatgagctgc cgcgagaact cgtccagctg ctccaggcag atttccatgt cgtgcacgaa
     2581 gatcacccga tcttcgaacg tggtgacgcg atatttggtc acggttgcgc gaaagaactc
     2641 tctccccagc agcgacggca tcctgccagt ttccagcgag tagcgcatat agcgccgcag
     2701 catcgccaca gttcttccgc ggtacatgcg ccgctcacgc tcgtgctccg gatccagttc
     2761 ggcgccgact tcgggctgcc catctcccgc attcttcgcg gggagcgagt tcacggcagg
     2821 aaactggtgc acgggaagtc cggggagcag aatctcgtcc gcgtactcct gctcccttcg
     2881 cttctcccgg cgtcgccagc gaatttcttc ccccggcgga actgtcctct tttccgccga
     2941 ggccgcgatc cacgcgatca tcccgctcat gccgcacctc cggccgcttc aaagtccagg
     3001 tcgtcggtgt cgtcgacttt gaaaggaaga ttcccgacca gcgaccgccg ctgcaccggc
     3061 aggctcaccc gggcgggctt tttcttcgga atcggcaagt ccgcaatgcg cggctggcag
     3121 ttctcgcagt agaccactcc cgcgccgcac tcgcgcagcc acaggcctcc gcagtgctcg
     3181 caatacttca gttccatgcg tacgatttcg tcgtctccgt ctcttcgatt gcttttcatc
     3241 gccccacctc ccgatcagtt gactgaaacc ccagttgaga aaaatccgcc tcgctccgtc
     3301 ggcctcctct acggccgccg aaggcgcagg gccagaaaga caaaagccct gcgctgatct
     3361 ccgcgagagg tgcagaaacc gaacgcaggg ctcgtttctc ttacgatggc ctgcctgtgc
     3421 tatttagttt tgctgagttt cgaaaaaacg gttacatcct gtaccgaacc ctgatcatcg
     3481 ccgctaacag ccctccgctt ctcacagttt gtgcgcgatc tcatcgtcgg ccccgccgcc
     3541 ctccacccgt tggcggaagt aaccttcctc gtagccggac ttgagtacgc gcccgttttg
     3601 catcactacg ctcacccgtc ctgtgaagcg aagcgacaac aacagccgct ccatggatgc
     3661 cgacagaact tccgccgacc gcactttccg gcgaaattcc ccgaagtctc tttcctcact
     3721 cgagttgtct gcatgtgtgc tcatcgcatt ccaccatgga gcatagtgac ccatactttg
     3781 cggcgatcct atagctctgg gttttctcag tcaaggttgg gtgaacaccc ctccttgcct
     3841 cctgtggaaa actggtccct tagcgcgcgg ctataggccg gtgctatact ccgcccatga
     3901 agttcaaggt cctgcaagat aatcttcgta agattctgcg ggatcgcatc gacgccggcg
     3961 aactcaccgg cctccggctg gcccggctaa cgcgcttcaa gcaagcccac atctccaact
     4021 ttctcaacaa gaagcgcggg ctgagcctgg agggcatgga caaggttctc tccgtacagc
     4081 acctctccgt gctcgatctg ctcgaccaag ctgaggtcaa ccaacgcgcc tcgatcctgc
     4141 cgcccagcgg agacaagttc gagaacgtca tcctcgccga cgccgctact gcggctacca
     4201 ggcgctcgat caccgcccgg caggtgaagg aaatcctcaa gttcaaaaaa agcttcctgc
     4261 aacgactgaa gcccaagacc gtgggtaacc gctccggctg gcagcgcttc gtgctcatca
     4321 aagtcgatcc tgccgatgcc cgcgccatgc atccccgcct cattcccgga gccgtcttgc
     4381 tcctcgaccg ccattacaac tcactccggc gttatcgcac gggcgcgtta aatatgtacg
     4441 ctgttctgaa aaacgatact tgcgcgatcc gctacgtcga agccgcagaa agttgtctaa
     4501 tacttcgccc gcataatccg catgcgccca tcgaagtcat tccggttgaa gacggcaaga
     4561 gcgccgagga ttacatcgtc gggcgcgtct gccatgcagg actggaaatg taacgccgtc
     4621 gcccctagcg gatcagagtg cccccgggca acccgctccg gttcaagctg ctcggccccc
     4681 gcaagcacaa cgctcgccgc tgttcgggct gcgagccatt gaaatcgcag caatttgtat
     4741 tggcgcgttt tttgctgatc gcgcctctgg tttccgcttc gttgatgtat ctcgcactcc
     4801 accatccgta ggaccgtccg gaaactcgtc aaagagcggc acattgtgga tgaatgccgg
     4861 cactgcaacg ttccgcgcag gtattgcctg tattggtaag gcttttacaa tcaattagat
     4921 atgacagttg ccagggaatc ttgtagaaaa tgcttgacaa gatatatagt gttatagtta
     4981 agtataacag taatggcgag cctaacttca ctttcgactt gctttcggcc cggcggcgag
     5041 ccggaacctg gctctcaagg agaatctgcc atgaaatctc atcgcccttt gcgcccgttt
     5101 gcagtgctgt ttctcaccac cgtggctttc gcgcagatgg ccatgcaatc gcccgccgta
     5161 caagcgtccg acgcgcagaa gtccttcgac aaactgaaaa ccctggccgg
//
LOCUS       DASPUL010000268         4531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1412316, whole genome shotgun sequence.
ACCESSION   DASPUL010000268 DASPUL010000000
VERSION     DASPUL010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412316"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VEJ00_06645"
     CDS             <1..1372
                     /locus_tag="VEJ00_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXX00874.1"
                     /translation="GHGVVHKLIAEALEDLGVQDQTILVSPVGCSVFAYYYFDVGNVQ
                     VAHGRAPAAATGLKRACPDKIVIAYQGDGDLAAIGTAEIIHAANRGEKITVFFVNNAI
                     YGMTGGQMAPTTLVGQKSTTSPWGRRPVNEGFPLHMAELLSSLEAPVYIERVALSDNK
                     NIMKARRAVRKALELQRDGAGFTFVEILSPCPTIWGKDPVDARKWVAEKMIPSFPLNV
                     FRDKKIEIPNNNVPPHKTVAELIGEDGKKEEKAAGEGVVPRRHHHFRDLGIKIAGFGG
                     QGVLLLGQLLAEMGMREGLEVSWLPSYGPEMRSGSAHCHVCLSKDRIGSPLLSRPQIL
                     IAFNEISLRKFAAEVVPGGIILYGREQLPEDLNIPHAQIVCVPASEIADKIGSAKVAN
                     VVMMGALLEETECLSPATAMKVLEAKVKNQALLELDRKALDAGRVYIDHTVAVGAVTQ
                     ADGFAQ"
     gene            1743..2447
                     /locus_tag="VEJ00_06650"
     CDS             1743..2447
                     /locus_tag="VEJ00_06650"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXX00875.1"
                     /translation="MARLPRVVVVGVAHHVTQRGNARQVVFDDAADRLTYLALLREHS
                     RLASLSVLGYCLMSNHVHLIATPHRAEALAQTLKHTHGRYAAYRNVQDRSNGHVWQGR
                     FYSCPLDDSHLWKALRYVELNPVRAGLVVTAEQWRWSSTRAHFAGCGDALLDMSAWQK
                     RWSPDEWRRYLSNGEAPAEIESLRQYTHTGRPLGSAEFVADLERSTSRPLAARKGGRP
                     RKLPTDPGSLVSESVA"
     gene            3031..3990
                     /locus_tag="VEJ00_06655"
     CDS             3031..3990
                     /locus_tag="VEJ00_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXX00876.1"
                     /translation="MALVQDPVKKEEQRVLSAISLKNVLFATDFSPTSEAALPYATAI
                     CRRFGATLHAAHVISDASLLMMTGGVDYVTMGTIYDDAQNEAKGKLEHLADRFEGVPH
                     RSHVCHGQVWKALARILEENEIDLIAVGTHGRTGLGKLLLGSVAEDILRHAPCPVLTV
                     GPKVSGRARLPEYPADGRDLAPAELELRHILLATNLVRQAEPAVRAAVAMAGEFESRL
                     TLLHVIEDYTELGTRTSQIEEGLRYLKAMVPTDAPLQYVPETLIEFGSAWDGILKVAD
                     ERESDLIVLGARTAREVGTTHLPWSTAHHVIAHASCPVLTVRG"
     gene            4183..>4531
                     /locus_tag="VEJ00_06660"
     CDS             4183..>4531
                     /locus_tag="VEJ00_06660"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HXX00877.1"
                     /translation="MPIAAKPAVERLRITLRGAVQGVGFRPFVYRLASDMALTGWVLN
                     SSAGLVVEVEGSAEQLSRFEQRLERERPRASVVTLRESARIAPQGSTGFEIRASDSDT
                     GKTVNVLPDLATCT"
BASE COUNT          839 a         1405 c         1393 g          894 t
ORIGIN      
        1 cggccacggc gtggtccaca agctgatcgc cgaagctctc gaagatctgg gcgtgcagga
       61 tcagaccatt ctggtgagcc cggtcggctg ctcggtgttc gcttactact acttcgacgt
      121 gggcaacgtg caggtcgcgc atggacgcgc ccccgctgcc gccaccggcc tcaagcgtgc
      181 ctgccccgac aagatcgtca tcgcctatca gggagacggc gacctggcgg cgatcggaac
      241 tgccgagatc atccacgccg ccaatcgcgg cgaaaaaatc acggtcttct tcgtcaacaa
      301 tgcaatctac ggcatgaccg gcggccagat ggcgcccacc acgctcgtcg gccagaaaag
      361 cacgacttcg ccctggggac gccggcccgt caacgaaggt ttcccgctgc acatggccga
      421 gctgctgtcg tcgctcgaag cgccggtcta catcgagcgc gtcgcactca gcgacaacaa
      481 aaacatcatg aaagcgcgcc gggccgtgcg caaagctctc gagctgcagc gcgacggagc
      541 gggattcacg ttcgtcgaga ttctttctcc ctgcccgacg atctggggca aggatccggt
      601 cgacgcgcgc aagtgggtgg ccgagaagat gattcccagc ttcccgctca atgtcttccg
      661 cgacaagaaa attgagattc ccaacaacaa tgtgccgccg cacaaaaccg tcgccgagct
      721 gatcggtgaa gatgggaaga aagaagaaaa ggccgcgggc gagggagttg ttccgcgccg
      781 ccatcatcac ttccgcgacc ttggcatcaa gatcgccggc ttcggcgggc agggcgtgtt
      841 gctgctcggc cagcttctgg cggaaatggg catgcgcgag gggctggaag taagctggtt
      901 gccctcctac ggccccgaga tgcgttcggg cagcgcccac tgccatgtct gcctgtcgaa
      961 agaccgcatc ggctcgccgc tcttgtcgcg gccgcagatt ctgatcgcct ttaacgaaat
     1021 ctcgctgcgc aagttcgccg ccgaggttgt tcccggcggc atcatcctct acggacgcga
     1081 acagcttccc gaagacctca acatcccgca cgcccagatc gtctgcgtcc cggcctcgga
     1141 gattgcagac aagataggct cagccaaggt cgctaacgtc gtgatgatgg gagccttgct
     1201 cgaagaaacc gagtgcctgt cgccggccac cgcgatgaaa gtgctggaag cgaaagtcaa
     1261 gaatcaggcg ctgctcgaac tcgatcgcaa agccctcgat gcgggccgag tgtacatcga
     1321 tcacaccgtc gcagtaggcg ctgttacgca agctgacggt tttgcgcagt agaagaagta
     1381 gaaaaggtta tccccgggga aagggtgccc cattctaacg tcgcgttctt tgcgacgtta
     1441 gggtgggatt ttgattctcg tactcaaaca aaatccaggc ggatcgccca cctgatcggc
     1501 gttcgtgatc tcccacgcca ggacgaaaag ggggaacgtc ccgtctgtcc ctgcgtttcc
     1561 actgtttccg ttccaaatcg gacctgtcct gttctgggca cattttgtta tctttcggga
     1621 aatcggggac agacgggacg tttccctgtt tccgttccaa atcggacctg tctttaccaa
     1681 ctttgcagcc agaccaatcg aagcgcggcc ctgttctggg cacattttgt tatctttcgg
     1741 gcatggcccg gcttccccgt gtcgtggtcg tgggcgttgc tcatcacgtc acccagcgtg
     1801 gcaatgcgcg gcaagtcgtc ttcgacgacg ccgccgatcg cctcacgtat ctggccttgc
     1861 ttcgcgaaca ttcgcgactc gcttcgcttt ccgtgctcgg ctactgtttg atgtccaatc
     1921 atgtgcacct gatcgcaacg ccacaccgcg ccgaggctct ggcgcagacc cttaagcaca
     1981 ctcatgggcg ctacgccgcc taccgcaacg tgcaggaccg ttctaacggc cacgtgtggc
     2041 agggccgatt ctactcttgc ccactggatg actcgcattt gtggaaggca ttgcgctatg
     2101 tcgagttgaa cccggtacgc gccggtctgg tggtgaccgc ggagcagtgg cgatggtcca
     2161 gcactcgcgc ccacttcgca ggctgtggcg acgccttgct cgacatgagc gcttggcaaa
     2221 agcgctggag tcccgatgag tggcgtcgct atctgtccaa tggggaagcg ccggcggaaa
     2281 tcgaaagctt gcgtcagtac acgcacacag ggcgtccctt gggctcagcg gagttcgtcg
     2341 ctgacctgga acgatccacg tcccgaccgc tggctgcgag aaaaggagga cgccccagga
     2401 agttgcccac cgatccgggg tcgctagtct ccgagagcgt cgcctagaat caaaaactcg
     2461 ggaacgttcc gtctgtcccc ggtttccgtc cccggtttcc tccatggtag acaggtccga
     2521 ttcgcgcagg ggcgtgtcgt caagaaacgg gccggccagt accagcttgc cctcctgggc
     2581 cagcttgtgg atgttcgcca tgtgcgcctc ctgcagcttt gcaagagcgg cggcgtcgag
     2641 ttggggtgca tccgggggcc ggcgcaaaag agccagttgg tagaagaagt actgctcggg
     2701 ctggacggtg gaggtggcgg gcgtggattg tccgttggcg atcgtcacca gcgctgccac
     2761 gacccccagc atccgtaagc tacggttggc tttcatcgac gggttctcct gatcggacga
     2821 cgccacaggg gcttccttcg actgtcctga cgcatcggcg gggttgactg gcggggcgcc
     2881 tcgattgacc cgtgatccac cgccgtatca gcataagaat aaaggcatct taagtgcaaa
     2941 ggtgatggga atcacagaaa ctcgtgatac tggccctatc gaaaatggcc cgactgtttg
     3001 atcctgacac cagggttgtg gaggtgtcct atggcgctcg ttcaggatcc agtcaagaaa
     3061 gaggagcaac gcgtcctctc tgccatctcc ctgaaaaacg tactgtttgc aactgatttc
     3121 tcgccgacct cggaggcggc cttgccctac gccaccgcca tctgccggcg ctttggcgca
     3181 acgttgcatg ccgcgcatgt gatttccgat gccagcctcc tgatgatgac cggaggcgtc
     3241 gattacgtca cgatgggaac catctacgac gacgcgcaga atgaggccaa agggaagctc
     3301 gagcacctgg ccgatcgctt cgaaggagtt ccgcatcgct cgcacgtttg ccacggccag
     3361 gtctggaagg cgctggcgcg catcctcgaa gagaacgaga tcgacctgat cgcggtggga
     3421 actcacggcc gcaccggcct gggcaagctg ctgctcggat ccgtggccga agacattctg
     3481 cggcatgcgc cctgccccgt gctcacggtc ggcccgaagg tcagcgggcg ggcccggctg
     3541 ccggaatatc cggccgatgg aagagacctg gcgccggccg aactggagct gcgccatatc
     3601 ctgctggcga ccaacctggt gcgccaggcg gagccggccg tccgggcagc agttgcaatg
     3661 gccggggagt ttgagtcgcg gctcactctg ctgcacgtga tcgaggatta caccgagctg
     3721 ggtacgcgca ccagccagat cgaggaaggt ctgcgctatc tcaaagcaat ggttccgaca
     3781 gatgcgcctc tgcagtacgt gcccgagact ctgatcgagt tcggctctgc ctgggacggc
     3841 atcctgaaag ttgcggatga gcgggagtct gatctgatcg tgctcggcgc ccgcaccgcc
     3901 cgggaggtcg gcacaaccca tctcccgtgg tcgacggcac accatgtgat cgcgcacgcg
     3961 tcctgcccgg tcttgacggt tcggggctag ggtcgagttt ctcgctccca gttgcgcagg
     4021 ctgggttttc ctcgcgccct ttgcggctct tccttgcgcg ggttgcatcc tggtcatttg
     4081 cttgcgacct tggcgcgcgg ttgaatctcc agcgcgtgac ggatagcagc cttcgcgctc
     4141 caggtcttac aatctggcga tcaggcgcaa tgcgtgacgc ctatgccaat cgcagccaaa
     4201 cccgcggtcg aacgtctgcg catcacgttg cgcggcgctg ttcagggtgt cgggttccga
     4261 cctttcgtct atcgtctcgc atcggacatg gctctcaccg gctgggtgct gaattcgtcg
     4321 gccggcctgg tcgtagaggt cgagggttct gccgagcagt tgagccgctt cgagcagcgc
     4381 ctggaacgcg agcggcccag ggcctcggtg gtgaccctgc gcgaatcggc ccgaattgcg
     4441 ccccagggct cgacgggttt tgaaattcgc gccagcgaca gcgataccgg gaaaaccgtc
     4501 aacgtcctgc ccgatcttgc gacctgcacc g
//
LOCUS       DASPUL010000269         4262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1549254, whole genome shotgun sequence.
ACCESSION   DASPUL010000269 DASPUL010000000
VERSION     DASPUL010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549254"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1208)
                     /locus_tag="VEJ00_06665"
     CDS             complement(231..1208)
                     /locus_tag="VEJ00_06665"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HXX00878.1"
                     /translation="MSPAFDPRPWGTEDLSPIYPNHKFSEKIGESWLSGDDCKVANGP
                     LAGKTLARLSERYQRELVGEAARDPKRFPLLLKFLFPHEKLSVQVHPDDEQALRVGEP
                     WGKTECWYVAHAKPGSQIALGLKPGVTAAQLEEAIHQKRAEELLNWITVYAGEMIYVA
                     GGTVHTLGPGSVIVETQQQSDTTYRLYDYGRPRELHLEYGMAAVKEKVKSGKVIRPAP
                     SQIRGGANRYAPLVAAPYFVVDMFEAKEPLELATRDDSGKSSVQILVAVEGCAAIEAS
                     GMNPVTLARGDAVVVPACIKDFEVRPQWAVEFLRAYVPGKALPEPETRM"
     gene            complement(1343..2539)
                     /locus_tag="VEJ00_06670"
     CDS             complement(1343..2539)
                     /locus_tag="VEJ00_06670"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXX00879.1"
                     /translation="MTTATTEALRLTDRINRIEPSATMAVVAEADKLRSQGIDVVDFG
                     AGEPHFATPQHIKDAAIAAIQSNFTKYTAVAGTAELRDAIAHRHAVDFDSDYRREEVI
                     ASAGGKQALFNAIQVLVDHGDEVILPVPYWVSFKDIVRYAGGTCVLLEADEAQGFRVT
                     AEMVARLITSRTKAIILNSPSNPSGAVMSPDDMTEVSRLAHQRGIWVLSDECYVYLNY
                     SGKNFSVGALREYKERFVVLGSLSKTYAMTGWRLGYALAPTGVVSAMAKLQSQSTSNP
                     TSIAQKAAVTALKGPQECVEQMRREYIQLRDHVVKGLRSIPGVSCTLPEGAFYAYPNV
                     SSFFGRGGLKSASDVAGRLLREAHVATVPGEGFGTSDHIRVSYATSKAELDRGLERMR
                     KFFAAL"
     gene            complement(2536..3081)
                     /gene="coaD"
                     /locus_tag="VEJ00_06675"
     CDS             complement(2536..3081)
                     /gene="coaD"
                     /locus_tag="VEJ00_06675"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HXX00880.1"
                     /translation="MNKGIAIYPGSFDPPTNGHLDLIQRGSKIFEELVVAILRNSEKS
                     PMFSVAERADMLRELTADLSNVRIDTFDGLMVEYAKSIDAMCVLRGIRAISDYEYELQ
                     MALMNRKIEPTLETVFMMPADKYSYVSSRLVREVAQAGGPIKGLVPEVVEQKLREKLE
                     PAYKLQDTHVMELPRKRKRKA"
     gene            complement(3229..>4262)
                     /gene="recA"
                     /locus_tag="VEJ00_06680"
     CDS             complement(3229..>4262)
                     /gene="recA"
                     /locus_tag="VEJ00_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXX00881.1"
                     /translation="GKGSIMRLGSKEAIVPISVISTGAISFDAALGVGGVPRGRVIEI
                     FGPESSGKTTIALQVIAEAQRTGGMAAFVDAEHALDAGYAKKLGVDVDNLLVSQPDYG
                     EQALEITEALVRSNAIDVLVVDSVAALVPKAELDGEMGDSHMGLQARLMSQALRKLTG
                     TVSKSRTCLIFINQIREKIGVMFGNPETTTGGRALKFYSSVRVDIRRIAAIKEGDVVT
                     GSRTKVKVVKNKVAAPFREAEFDILYGEGISREGDLLDLAVNNNIVEKSGSWFSYKGE
                     RIGQGRENARQFLKDNKDVMAKLDAEVRKAMGLITAQVQPAAAQAAPGAKVATMPEAV
                     KAPIVAARR"
BASE COUNT          830 a         1364 c         1201 g          867 t
ORIGIN      
        1 gcgagcaatt gcttggcgcg cttcttgcgg ctcagcggcc agaagcgcgt gccgcgccct
       61 ccggccagaa tcacgggata aaagtctgag ttcttcgcca cgggatttcg caagtcaaaa
      121 ttgcagattt cagattgcag atttcagctt tgccagcgtc aatctcgtct cggcaccagg
      181 caggagccaa tcgtttaatc tgaaatctaa aatctgcaat ctgcaatgga ctacattctc
      241 gtttccggct cgggcagggc cttgccggga acatatgccc gcaggaattc caccgcccac
      301 tgcggacgca cctcgaaatc cttgatgcag gctggaacga cgacggcgtc tcctcttgca
      361 agggtcacag gattcatccc cgatgcctcg atcgcggcgc agccctcaac tgccaccaga
      421 atctgcacgg aactcttgcc tgaatcatcg cgggtcgcca actccagcgg ctctttcgct
      481 tcaaacatgt ccaccacgaa gtaaggagct gcgaccaatg gcgcgtaccg gttcgctccg
      541 ccacgaatct gcgacggcgc cgggcggatc actttgcccg acttcacttt ctccttcacc
      601 gccgccatgc cgtattccag atgcaattcg cgcgggcgcc cgtagtcgta caagcgataa
      661 gtcgtgtcgg actgctgctg cgtctccacg atcactgatc ctggtcccag tgtgtgcacc
      721 gtgccgcccg cgacgtagat catttcaccg gcatagacag tgatccagtt cagcagttcc
      781 tcggcgcgtt tctgatgaat cgcctcctct agttgcgctg cagtaacgcc cggcttgagg
      841 cccaaggcaa tctgcgagcc cggcttggca tgcgccacat accagcactc ggttttgccc
      901 catggttcac cgacgcgcag cgcctgctcg tcatcgggat gcacctgcac actcaacttt
      961 tcatgcggga acaggaactt cagcagcagg ggaaagcgct tcggatcgcg tgccgcttca
     1021 ccaacgagct cccgctgata tcgctcgctc agccgtgcga gcgtcttgcc cgccaggggg
     1081 ccatttgcaa ccttgcaatc gtcaccgctc agccaggact cgccaatttt ctccgagaac
     1141 ttgtgattgg gataaatcgg cgacaggtct tccgtgcccc aggggcgcgg gtcgaacgcg
     1201 ggagacatca atagcggata taaatccccc atagagacta acgctaacag aaaaggccgc
     1261 ccaccaacgg aacggccttt gccgaacccg gggttctcct gtggccaagc gatttgtaat
     1321 ctcgctgact ggaaaaacag cactacaacg cagcgaagaa cttccgcatg cgctccaggc
     1381 cgcgatccaa ctcggccttg gacgttgcgt aagagacccg aatgtggtcg ctcgtgccga
     1441 aaccttcgcc cggaacggtg gccacgtgcg cttcgcgcag caggcgtccg gccacgtcgg
     1501 acgcggactt cagtccgccg cggccaaaaa acgacgagac gttaggatag gcgtagaagg
     1561 ctccctcagg cagcgtgcag ctcacgcccg ggatcgagcg cagcccctta accacatggt
     1621 cgcgaagctg aatgtactcg cgccgcatct gctcgacgca ctcctgcggt cccttcaagg
     1681 cggttaccgc agccttctgc gcgatcgacg tcgggttcga cgtcgactga ctctgcagct
     1741 tggccatggc gctgaccacc cctgtcggcg ccagcgcata cccgaggcgc cagccggtca
     1801 tggcgtatgt cttcgagagc gatcccagca ccacgaaacg ctccttgtat tcgcgcagcg
     1861 cgccgacgga aaaattcttg ccagaatagt tcaaatagac gtagcactca tccgacaaca
     1921 cccagatgcc acgctggtgc gccagacgac tcacttcggt catatcgtcc gggctcatga
     1981 cggcgccgga ggggttcgac ggtgaattga gaataatggc cttggtccgc gatgtgatca
     2041 gccgtgcgac catctccgcc gtgacgcgga atccctgcgc ctcgtcggcc tcgagcagaa
     2101 cgcatgtccc gcccgcgtag cgcacgatgt ccttgaacga cacccagtaa ggcacgggaa
     2161 gaatcacttc atcgccgtga tcgacgagaa cttgaatcgc gttgaataac gcttgcttgc
     2221 cgccagcaga agcgatcact tcctcgcggc gatagtcaga atcgaaatcg accgcatgac
     2281 ggtgcgcaat cgcatcgcgc agttcggccg taccggcgac cgctgtgtac ttcgtaaaat
     2341 tgctctggat cgccgcgatg gctgcgtctt tgatgtgctg cggcgtggcg aagtgcggct
     2401 cgcccgcgcc gaagtcgact acgtcgatgc cttgcgagcg cagcttgtct gcctcggcca
     2461 caaccgccat ggtggcggaa ggttcaatgc ggttaatgcg gtcggtgagt cgcagcgctt
     2521 cggtggtcgc ggtcgtcatg ccttcctttt ccttttccgg gggagttcca tcacatgcgt
     2581 gtcctgcagt ttgtacgcgg gctccaattt ctcgcgcagc ttctgttcca caacttccgg
     2641 aacgaggcct ttgatcggac cgccggcctg cgccacttcc cgtaccagcc gcgagctcac
     2701 gtaggaatac ttgtcggccg gcatcatgaa cacggtttca agtgtaggct cgatcttgcg
     2761 gttcatcagc gccatctgca gctcatattc gtagtcgctg atggcgcgga tgccgcgcag
     2821 cacgcacatg gcatcgatcg atttcgcgta ctcgaccatc aagccgtcga aggtgtcgat
     2881 gcgcacgttg ctcaggtcgg cggtcagctc gcgcagcatg tccgcccgct cggccacgct
     2941 gaacatcgga cttttctccg agttccgcag gatcgccacc accagctcct caaagatctt
     3001 gctgccccgt tgaatcaggt cgaggtgccc gttggtcggc gggtcgaacg agccgggata
     3061 aatggcgatg cctttgttca agtgtcctct ttggaaggct gagatgaaca gatctcgcaa
     3121 cagtggagca aagaggattt tatgcgggcg cgaccaacaa tggcaatcgg ggaatagtgg
     3181 agggagatcc gggcgcagcc tgatcgccgt gcccggaggt aggcaaattc agcgtcgtgc
     3241 tgccacaatc ggcgccttca ccgcttcggg catggtggcg actttggctc cgggtgctgc
     3301 ctgagccgcc gcgggctgga cctgggccgt aatcaatccc atggctttgc gcacctcggc
     3361 gtcgagcttg gccatgacgt ccttgttgtc tttcaggaac tggcgggcgt tttcgcgtcc
     3421 ctggccgatg cgctcgcctt tgtaactgaa ccacgagccg gacttctcga cgatattgtt
     3481 gttcactgcc aggtccagca gatcgccttc gcgcgaaatg ccttcgccgt aaagaatgtc
     3541 gaactcggcc tcgcgaaacg gcgcggcgac tttgttcttg acgaccttca cttttgtccg
     3601 cgaaccggtg accacgtcgc cttccttaat ggcggcaatg cgacgaatat cgacgcggac
     3661 ggaagaataa aacttcagtg cgcggccgcc ggtcgtggtt tccggattgc caaacatcac
     3721 gccaatcttc tcgcggatct ggttgatgaa gatcaggcac gtacgcgact tcgaaaccgt
     3781 tccagtcagc ttgcgcagcg cctgcgacat caaccgcgcc tgcaatccca tgtggctgtc
     3841 gcccatttcg ccgtcgagtt ccgccttcgg caccagcgct gcgacagagt ctacgaccag
     3901 aacgtcaatc gcgttcgagc gcaccagcgc ttcggtaatc tcaagcgcct gctcgccgta
     3961 gtccggctgc gaaaccagca ggttgtccac gtccacgccc agttttttgg catagcccgc
     4021 atcgagcgcg tgctcggcat ccacaaacgc ggccatgccg ccggtgcgct gcgcctcggc
     4081 aatcacctgc agcgcaatgg tcgtcttgcc ggaagactcc ggcccgaaaa tttcaatcac
     4141 gcggccgcgc ggcactccgc ccacgcccag cgccgcgtcg aaagaaatcg cgcccgtcga
     4201 gatcaccgag atggggacga tcgcttcctt cgatcccaac cgcatgatcg agcctttgcc
     4261 aa
//
LOCUS       DASPUL010000270         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1823598, whole genome shotgun sequence.
ACCESSION   DASPUL010000270 DASPUL010000000
VERSION     DASPUL010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823598"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(307..1701)
                     /locus_tag="VEJ00_06685"
     CDS             complement(307..1701)
                     /locus_tag="VEJ00_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HXX00882.1"
                     /translation="MPTEFAGSQYIIEVIYLIASALFILSLKWMSSPSTARHGILAGE
                     IGMVLAIGGTLLRHGIVDWKWIIIALVLGSGIGVPLGMVQMTAVPQRTALSHAFGALC
                     VTLVGTAEFYLGTQTTSKFGMSVLFLEVILGGLTFTGSLIAAGKLQEVLPQRPITYKG
                     QNVVNLTFLAIAIALAVYLILHPEAKQFFPAVVGIALIFGIFLVIPIGGADMPTVISL
                     LNSYAGLSAAAMGFVLNSKLLIIAGALDGSSGFILSVIMCKAMNRSFTNVLFGAFGQV
                     QPTAAAGQEARTVRSATPEEAASILAAANKVVVVPGYGMAVAQAQHKVRELYDLLTKK
                     GVEVRFAIHPVAGRMPGHMNVLLAEADIPYDRLSEMDDINGDFPQTDVALVIGANDVT
                     NPAARTDQSSPIFGMPILDVDKAHTVMVIKRSMNPGFAGIDNPLYYLDNTLMLFGDAK
                     QFVGAIVRELGGGH"
     gene            complement(1721..2014)
                     /locus_tag="VEJ00_06690"
     CDS             complement(1721..2014)
                     /locus_tag="VEJ00_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HXX00883.1"
                     /translation="MGSTIIDALFVFMLAAFIGFEVIRHVSPLLHTPLMSLTNALDAI
                     AVVGAIVLVGERKSTFATVLGVIAIVAATSNVVGGFFITDRMLRMFKTSRPSK"
     gene            complement(2078..>3052)
                     /locus_tag="VEJ00_06695"
     CDS             complement(2078..>3052)
                     /locus_tag="VEJ00_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HXX00884.1"
                     /translation="ADIVVQVLCYGSNDITGKADLPLMRRGQALIGFLRPLGSAEVVE
                     QIAHAGVTAFSVEFMPRTTRAQSMDALSSMATCCGYKAVLLAAETSPRIFPMMTTAAG
                     TITPARVFVIGAGVAGLQAISTARRLGAVASAYDMRPAAKEQVQSLGGRFVELPIEAK
                     DAQDARGYGTAQDESFYARQRELLTRVVAESDIVITAAVIPGKKSPVLITADMVKGMA
                     PGSVIVDLAAERGGNCELTQTGKTVVQHGVTIIGAINLASSVPYHASQMYARNLTTYL
                     TYMVKDGKLQVNLQDEIVRETLVTSGGEIVHARVREFFKLPALTPAGS"
BASE COUNT          646 a          904 c          946 g          556 t
ORIGIN      
        1 acgccgggct tgagacgaaa ggggatccca gtttggcggg atagctgttc ctgaaaccgt
       61 agaatgtcgg ctccaacagc atttcggtaa ccggtggacg acaccgcgaa cgactgcgtg
      121 atgagtaact ggcctgtgcc aagctgaata ttggcgggca tgggcatgag attcagctga
      181 ggctgggatt gggctactgc caggggcacc ggaaagagag tcgcgagtag gaaaacaagt
      241 ttatgcatag gtctacgaaa gagtatgccc taaaggcggg gtggaaaacg gcggagcgaa
      301 aggaactcag tgtcccccgc ccagctcgcg cacaattgcc cccacaaact gcttggcgtc
      361 accgaagagc atgagcgtgt tatcgaggta atacagcggg ttatcaattc cagcgaagcc
      421 cgggttcatg ctgcgcttga tcaccatcac ggtatgagcc ttgtcgacgt cgaggatggg
      481 catgccgaaa atggggctcg actgatcggt gcgcgcggca gggttggtca catcgttcgc
      541 gccgatcaca agggcaacgt ccgtttgcgg gaagtcgcca ttgatgtcat ccatttcact
      601 gagccggtca tagggaatgt cggcttcggc gagcagaacg ttcatgtggc cgggcatgcg
      661 tccggcaaca ggatggatgg caaagcggac ctccactcct ttcttggtca gcaagtcgta
      721 aagctcgcgc accttgtgct gcgcctgcgc gacggccatg ccatagccgg gtactacgac
      781 gactttgttc gccgccgcca gaatggacgc ggcttcttca ggcgtcgcgc tgcgaacggt
      841 cctggcttcc tgaccggcgg ccgcggtagg ttgcacctgg ccgaatgcgc cgaaaaggac
      901 attcgtaaat gaccggttca tggccttgca catgatcacg gagaggatga agcccgacga
      961 cccgtcgagc gctccggcga tgatgagcag cttggagttg agaacaaatc ccatggctgc
     1021 ggcggaaaga ccagcgtagg agttcaacag cgaaatcacc gtcggcatgt cggcgccgcc
     1081 gatcgggatc acgaggaaga ttccgaagat gagcgcgatg cccacaacgg ccgggaagaa
     1141 ctgcttggct tcgggatgca gaatgagata gacggccaga gcgatggcaa tggctagaaa
     1201 agtcaggtta acgacgttct ggcctttgta tgtgatcggc cgctgcggga gcacttcctg
     1261 cagtttgccg gcggcaatca agctgccggt gaacgtcagc ccgccaagga tgacttccaa
     1321 aaacagcacc gacatcccga actttgacgt ggtctgcgtt cccagataaa actcggcggt
     1381 gcccaccagc gtgacgcaga gcgcaccgaa ggcgtggctg agcgccgtcc gctgcgggac
     1441 ggcggtcatc tgcaccatgc ccaagggaac gccgatgccg gaaccgagca ccagcgcgat
     1501 gatgatccac ttccagtcca cgatgccgtg gcgcagcagc gtgccgccga tagccagcac
     1561 catgccgatt tcgcccgcca gaattccgtg gcgggcagtg gagggagagc tcatccactt
     1621 cagcgaaaga atgaagagtg cgctggcgat gagatagatg acttcgatga tgtactggct
     1681 tccggcgaac tcggtcggca tagattatgg cttcacgaaa ctatttcgac ggacggctgg
     1741 tcttgaacat cctgagcatg cggtcggtga tgaagaatcc gccgaccacg ttgctggttg
     1801 cggccacaat ggcgatcacg cccaagacag tcgcaaatgt gctctttctt tcgcctacca
     1861 gcacaatggc gccgacgacc gcaatcgcgt cgagggcgtt ggtcagcgac atcagcggcg
     1921 tgtgcagcag aggcgacacg tggcggatca cctcaaaccc gatgaaggcc gccagcatga
     1981 atacgaacag cgcgtcgatt atggtggaac ccatgcgata cctcagcttt cagcgatcag
     2041 gttgtcaatt ccgaagaacg aacagtccgg catcgactca cgaccctgcc ggcgtaagtg
     2101 ccggcagttt gaagaactcc cggacccggg cgtgcacaat ctccccgccg ctggtcacca
     2161 gcgtttcgcg cacgatctcg tcctgcagat tcacctgcag cttgccgtcc ttgaccatgt
     2221 aggtaaggta ggtcgtcagg ttgcgggcat acatctggct ggcgtggtac ggaacgctgc
     2281 ttgccaggtt gatcgcgcca atgatcgtga ccccgtgctg caccaccgtt ttccccgtct
     2341 gggtcaactc gcaattgccg ccgcgctccg ccgccaggtc gacgatcacc gaacccggcg
     2401 ccatgccttt caccatatcc gccgtaatga gcaccggcga ctttttgccg ggaatcaccg
     2461 cggcggtaat gacgatgtcg ctctcagcca cgacgcgggt cagcaactcg cgctggcggg
     2521 cataaaagct ctcgtcctgc gcggtgccgt agccgcgggc atcctgcgcg tccttggcct
     2581 cgatcggcag ttcgacaaaa cgtccgccga ggctctgcac ctgctccttg gcggcgggcc
     2641 gcatgtcgta cgccgaggca actgcaccta accggcgggc ggtcgagatc gcctgcagcc
     2701 cagctacgcc cgctccgatc acaaacaccc gagccggggt aatcgttccg gcggccgtgg
     2761 tcatcatggg gaagatgcga ggcgaggttt cagccgccag caggactgcc ttgtagccgc
     2821 agcacgtggc catggaagag agcgcgtcca tgctctgggc gcgcgtagtg cgcggcatga
     2881 attccacgga aaaggccgtg accccggcgt gcgcgatctg ctcaacgacc tctgccgaac
     2941 ccagcggccg caggaatccg atcagggcct ggccgcgacg catcaacggc agatcggctt
     3001 tgcctgtgat gtcgttcgac ccgtagcaaa gcacctgcac aacaatgtcg gc
//
LOCUS       DASPUL010000271         3431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_45941, whole genome shotgun sequence.
ACCESSION   DASPUL010000271 DASPUL010000000
VERSION     DASPUL010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3431)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3431
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45941"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="VEJ00_06700"
     CDS             <1..947
                     /locus_tag="VEJ00_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXX00885.1"
                     /translation="GCTMLTERRSCLRHEVHGPVFASFDGVTGGMILDLSEQGLAMQT
                     TVPLAADQEVHLRLDLPDSDALETTGYVAWADALGRAGVRFSELPVEARQRLDQWLAR
                     NDEAPSRKAPKLTLYPTFEPNRSVASRGDEKPQPQSISLEAEAGESGSQPLSNTVQYE
                     FHSLSSDLNAALRTISERARSLLRGGGAAIALIDNSSTTNKKMLCRASVGSGSPPLGT
                     PVDVNSGFSGECIRSGRAMRCDDAETDSRVSADACRRLGIRSIVAAPIRYEREVVGLL
                     EVFSPKSFAFDEGDLAVVERLAQTTILKVSQMQALTRA"
     gene            complement(984..1883)
                     /locus_tag="VEJ00_06705"
     CDS             complement(984..1883)
                     /locus_tag="VEJ00_06705"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXX00886.1"
                     /translation="MRRRLEYAAAWPFIKILGILPRRLSRAFAIGIAQAFYLFHGRLR
                     RVGMRNLSLAFPEKTEAERARILRGEFTSLGRQLAEVCQFPRYTLKNVEQVVVYDGLE
                     NYERARARGKGVLFLTAHFGAWELSAFAHSLHGHWLHVVMRPMDNPYLDRLIQSYRTM
                     YGNKTVGKDDFVRGLLAGMKAGETVGILMDTNMTPPQGVFVDFFGIPACTASGLARIA
                     LRTDAAVLPGFTIWDEALGKYRLRFDPAVELIRTGDLEADIIANTQKFTKIIEDYVRK
                     YPEQWLWVHRRWKTRPEGQAPLY"
     gene            complement(1966..2658)
                     /locus_tag="VEJ00_06710"
     CDS             complement(1966..2658)
                     /locus_tag="VEJ00_06710"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HXX00887.1"
                     /translation="MKKLVVLAILVSLCGFAAAADDQQKDSKATDRVKAAADVLDEIQ
                     GAPDKGIPQEILGSAECVAVVPSMLKGGFIFGAKYGRGIASCRTPKGWSAPAFFSVEG
                     GSFGLQIGGQAVDLVMLIMNENGMKNLLSSKFELGADASVAAGPVGRHAEGNTDWKMR
                     AEVLTYSRARGVFAGVTLNGAVIKQDKDSTREFYDHMVTFPAALKGEIDPPAAASPFL
                     TTLAKWAQEAAK"
     gene            complement(3011..>3431)
                     /locus_tag="VEJ00_06715"
     CDS             complement(3011..>3431)
                     /locus_tag="VEJ00_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXX00888.1"
                     /translation="RIIVTGSGIDEETILKAIASGAKGYVDEAATPAEFAQAIRVVNQ
                     GSVWAPRRVLSMFIERVSSAPGRIFPAGRVTFTDREKEVLEMLVAGRSNKEIGSQLGI
                     EERTVKAHVAKLMRKVGVQNRIALSVHAITHSLVTTK"
BASE COUNT          667 a         1103 c          998 g          663 t
ORIGIN      
        1 caggctgcac gatgctcacc gaacgacgca gttgcctgcg gcatgaagtg cacgggccgg
       61 tctttgccag cttcgacggc gtgactggcg ggatgattct tgatctcagc gagcagggtc
      121 tggcgatgca gaccacggtc cccctcgccg ccgatcagga ggttcacctg cgcctcgatc
      181 ttcccgactc cgacgcgctc gagaccaccg gctatgtggc ctgggccgac gcactcggcc
      241 gggctggagt acggttctcc gagcttccgg ttgaggcgcg ccagcgcctc gatcagtggc
      301 tcgcgcgcaa cgacgaagcg cccagccgta aggcaccgaa gctcactttg tatcccacgt
      361 tcgagccgaa ccggagcgtc gcatccaggg gtgacgaaaa gcctcagcca caatcgatca
      421 gtctggaagc ggaggccggg gaatcgggta gccagcccct ttcgaacaca gtgcagtacg
      481 aattccactc actctcctct gatctgaatg cggcgctgcg aaccatcagt gagcgcgccc
      541 gcagtcttct gcgcggcggc ggtgcggcta ttgccctcat tgataacagc agcacgacga
      601 acaagaaaat gctgtgccgc gccagcgtcg gaagcggcag cccacccctc ggaactccgg
      661 tagatgtcaa ttccggattc tcgggagagt gcatccgctc cggaagagcc atgcgctgcg
      721 atgacgctga aaccgactcg cgggtctccg ccgacgcctg ccgacggctc ggcattcgct
      781 ccattgtggc tgcccccatc cgctacgagc gcgaagtcgt cggattgctt gaggttttct
      841 ctcccaaatc gttcgcgttc gatgaaggcg atctggcggt cgtcgagcgc ctggcccaga
      901 ccacgattct caaggtgagc cagatgcaag ccttaactag ggcttagccg ctcgctctcg
      961 aaggcggggt ctccccacgc tctctagtac agcggcgctt gcccctccgg ccgtgtcttc
     1021 catctgcgat gcacccacag ccactgctcc ggatacttcc gcacataatc ttcaatgatc
     1081 ttcgtgaatt tctgagtatt cgctatgatg tcggcttcca gatcgccggt gcggatcaac
     1141 tcgactgccg ggtcaaagcg caggcggtac ttgcccagag cctcgtccca gatcgtgaat
     1201 ccgggcagga cggcagcatc ggtgcgcagg gcgattcgtg ccagcccgct cgcggtgcac
     1261 gcgggaatcc caaagaaatc cacgaagact ccctgtggag gagtcatatt cgtgtccatc
     1321 aggattccta cggtctcgcc tgccttcatc cccgccagca gtccgcgcac gaagtcgtct
     1381 ttgccgaccg tcttgttccc gtacatggtg cggtaactct ggatcagccg gtcgagatac
     1441 ggattgtcca tcggccgcat gaccacatgc agccagtgcc cgtgcaggga atgcgcaaac
     1501 gccgaaagct cccatgcgcc gaagtgggcg gtcagaaaca gcacgccctt gccccgcgcg
     1561 cgggcccgtt cgtagttctc gaggccgtca taaaccacga cctgctcaac gtttttcagg
     1621 gtgtatcgtg ggaactggca gacctcggcg agttgccgcc cgagcgacgt gaattcaccc
     1681 cgcagaatgc gtgcccgttc ggcttctgtt ttctcgggaa acgccaggga gagattgcgc
     1741 atcccaactc ggcgtaaacg accgtggaaa agatagaagg cctgtgcgat tccaatcgcg
     1801 aaggcgcggg agagccgacg cggcagaatg cccagaatct tgatgaacgg ccacgccgcc
     1861 gcgtattcga gccttcgacg catccggggt cagggatcag gggccagggg tcagtaagcg
     1921 ccttttatcc gaaaggctga cccctgacct ctggcccctg gccccttact tcgccgcctc
     1981 ctgcgcccac ttggccagcg tggtcagaaa cggactggcc gcggcgggcg ggtcgatctc
     2041 tcccttaagg gcagcgggga atgtcaccat gtgatcatag aactcccggg tgctgtcctt
     2101 gtcctgcttg atgacggcgc cattcagcgt caccccggca aatactcctc gagcgcggga
     2161 ataggtcagg acctcggcac gcatcttcca gtccgtgttg ccttccgcat gccgtcccac
     2221 gggtccggcc gctaccgacg catctgcccc cagttcgaac ttgctcgaga gcaaattctt
     2281 catcccgttc tcattcatga tcagcatgac aagatcgacc gcctggccgc cgatctgaag
     2341 tccgaagctg ccgccttcta cgctgaagaa cgcaggtgcg ctccaacctt tgggggtgcg
     2401 acagctggca atgccgcggc cgtacttggc gccgaagatg aatccgccct tgagcatcga
     2461 cggcaccacg gcaacgcatt ccgccgaacc caggatctcc tgaggaatgc ccttgtcggg
     2521 tgcgccctgg atttcgtcca acacatctgc ggcagcctta acccggtccg tcgctttcga
     2581 gtccttctgc tgatcatcgg ctgcggcggc aaagccgcag agcgacacaa gaatcgccaa
     2641 caccacaagc tttttcatgg gtcctcacca caaaacagaa ttcactggga tctggtattt
     2701 ggattgtaaa tgaagtcgcg ctgttgcatg ggccaaagcc cgtacggccg gaagacctct
     2761 gcgagggatt gccaactgac ggttcagaac tcgggactgg gagctggaca aaaaaatgcg
     2821 gggctgaaag cagtgaccct aatcgccact ctcaaccccg tctcccaggt tctgtggtag
     2881 gtgaagcgag tatgggtcag gtcgtcggcc tgaacaagat tacgaaggta agtggctgaa
     2941 agttacggcg agtcgtgagc tgggagccgc gagctacgag aggatctacc tcatagctcc
     3001 cagctcgcag ctacttcgtc gtgaccagcg agtgcgtgat agcgtgcacc gacagcgcaa
     3061 tccggttctg gactcccacc ttgcgcatga gcttggcgac atgcgccttg acggtgcgtt
     3121 cctcgatccc caactggctg ccaatttcct tgttggaacg gccagcgacc agcatctcca
     3181 ggacttcttt ctcgcggtcg gtgaaggtca cgcggccagc ggggaaaatt cttcccggcg
     3241 ccgacgagac gcgctcgatg aacatcgaca gcacgcgccg cggggcccaa accgaaccct
     3301 ggttgaccac ccggatcgcc tgcgcgaatt ctgctggcgt cgcggcctcg tccacatagc
     3361 ccttggcccc ggaggcaatc gccttcaaga tcgtctcttc gtcaatgcca gagccggtca
     3421 cgatgatccg c
//
LOCUS       DASPUL010000272         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1004478, whole genome shotgun sequence.
ACCESSION   DASPUL010000272 DASPUL010000000
VERSION     DASPUL010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004478"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2483)
                     /locus_tag="VEJ00_06720"
     CDS             complement(<1..2483)
                     /locus_tag="VEJ00_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXX00889.1"
                     /translation="MNRESQARFVAVFLFLLTLAATVFAGFNFQAERKVTAPDDGVEW
                     VEHGGRLVADQVETDGPGAKAGIKPGDQLVSVNGQEIKNTAGLERQLYRTGIWAKAGY
                     SLVRQSVTLDSTVILVPADRSLNNWLRLIALIYLGIGLYVLLRRWTAPGSTHFYIFCL
                     VSFIAYSFKYTGKFNDFDWTIYWSNIVAWVLQPALFLHFVLIFPEKREFVRRHPWALT
                     LIYAPGAFLLGVRLLAMRLLEASGRLSRGMDRLDVSYGAVFFILAAAALWHSYQRAST
                     TILRQQLKWVTRGTVLAVAPYTLFYVLSYVFPHAMAWLPEGAMKLSVLSLGLLPLTFG
                     YAIFRYRLMDVDLIFKRGVVYTLAAATVAGLYFALVAGVAWLVQMQKPATGAVGLILA
                     VVVTALLFDPVRKWIQDRVDQFFYRTRYDYRRTLLEFGRELSSETDLNALLSSALDRL
                     SRTLAVDRLAIFLNTGEEPERFVLSKSAGLVAPPQLDLSFLTVPRPEQAGHLFFENTH
                     QVPRETEGARQAIARLDLNYYIPCRAQQKTVAVLGLGKTVKGDFLSSEDVELLETLGG
                     YLGIAIQNGRLYASLQQKAAEYERLKDFNENIVESINVGVMALDMEDRIESWNAQMEV
                     MYALPRWQALTQPLQTVFPPEFVEEFYRIRQNPGISNLYKFRMRTPAGETRTVNVAIA
                     PLVTRKFQVIGRLVITDDITARVELEGQLSQADKLSSIGLLAAGVAHEVNTPLAVISS
                     YTQMLAKQLQGDPQKSGLLEKITRQTFRASEIVNNLLNFSRTSGSEFADIDINKVITD
                     TLALLEHQFKTAKIQVQSELTPAISPIQG"
BASE COUNT          541 a          825 c          742 g          468 t
ORIGIN      
        1 ttcccctgaa tcggagagat cgccggagtc agttccgact gcacctggat cttggcggtt
       61 ttgaactggt gctccagcag agccagcgtg tcggtgatga ccttgttgat atcaatgtcg
      121 gcaaactcgc ttccactcgt ccgcgagaaa ttcagcaggt tgttgacaat ctccgaagcc
      181 cggaaggtct gccgcgtgat cttttccagc aagccggact tctgcggatc gccctgcagc
      241 tgcttggcca gcatctgcgt gtacgaagag atcacggcca ggggcgtgtt cacttcgtgc
      301 gctacgccgg ccgccagcag cccgatcgac gagagcttgt cggcctgtga gagctggccc
      361 tcaagctcca cacgcgccgt gatgtcgtcc gtgatgacga gtcgtccgat cacctggaac
      421 ttacgcgtca ccagcggcgc gatggccacg ttcaccgtgc gcgtctcgcc cgctggagtc
      481 ctcatgcgga atttgtacag gttgctgatg cccggattct gccggatgcg gtagaactcc
      541 tccacgaact ccggcggaaa aaccgtctgc aagggctgag tcagcgcctg ccagcgcggc
      601 agcgcataca tcacttccat ctgcgcgttc cagctctcga tgcggtcttc catgtcgagc
      661 gccatcacgc ccacgttgat cgactccacg atgttctcgt tgaagtcctt caggcgctca
      721 tattccgccg ccttctgctg caacgaggca tacagacggc cattttggat ggcgatgccc
      781 agatacccgc ccagtgtctc cagcagctca acgtcttcgc tcgaaaggaa gtcgcccttg
      841 actgtcttgc ccagccctag gacggccaca gtcttctgct gcgcgcggca tgggatgtag
      901 tagttcagat ccaggcgtgc gatcgcctgc cgcgccccct cggtttcgcg cggcacctgg
      961 tgtgtgttct cgaagaacag atgtcccgcc tgctcgggtc gcggcacagt cagaaagctc
     1021 agatcgagct gcggcggcgc cactaggccc gccgatttcg aaagcacgaa ccgctccggc
     1081 tcctcgcccg tgttcagaaa gatggcaagc cggtccacgg cgagagtgcg cgacagccgg
     1141 tcgagcgcag aagaaagcag cgcgttcaaa tccgtttccg aactgagttc tcggccaaac
     1201 tcgagcagcg tgcgccggta gtcgtagcgc gtgcgataga agaactggtc gacgcggtcc
     1261 tgaatccatt tgcgcaccgg atcgaacaaa agcgccgtca ccaccaccgc cagaatcaat
     1321 cccacggcgc cggtggccgg cttctgcatt tgcaccagcc aggccacgcc cgccaccagc
     1381 gcgaaataga gcccggcgac ggttgcggcg gcgagcgtgt agaccacacc acgcttgaaa
     1441 atgaggtcca cgtccatcag ccggtagcgg aaaatcgcgt agccgaaggt cagcggcaga
     1501 agccccaacg acaataccga cagcttcatc gcgccttccg gcagccacgc catggcgtgc
     1561 ggaaagacgt aggacaacac gtaaaagagc gtgtaaggag cgaccgcaag taccgtcccg
     1621 cgcgtgaccc acttcaactg ctgccgcagg atggtcgtac tcgccctttg atagctgtgc
     1681 cacagcgctg ccgccgccag aatgaagaac accgcgccat acgacacgtc aagccggtcc
     1741 atgccgcggc tcaaccgccc ggaggcctcc agcaggcgca tcgccagcag ccgcacgcca
     1801 agcaggaaag cacccggcgc gtaaatcagc gtcagagccc acggatgccg ccgtacgaac
     1861 tcacgctttt cggggaatat caaaacgaag tgcaagaaca acgccggctg caacacccag
     1921 gcgacgatat tcgaccagta gattgtccag tcgaaatcgt tgaactttcc cgtgtacttg
     1981 aaggaataag cgatgaacga aaccaggcag aaaatgtaga aatgcgtcga tcccggcgcg
     2041 gtccagcgcc gcagaaggac ataaagaccg attcccaggt agatcagcgc aatcagccgc
     2101 agccagttgt tcagcgagcg gtccgccgga accagaatca cggtggaatc cagggtcacc
     2161 gactggcgca ccagcgagta gccggccttg gcccagattc ctgtgcggta gagctggcgt
     2221 tccagccctg ccgtgttctt gatttcctgg ccgttgacgg aaaccagctg atctcccggc
     2281 ttgatcccgg ccttggcccc gggtccatcg gtttcaacct ggtcggcgac caacctgccg
     2341 ccatgttcca cccactcgac gccgtcgtcg ggagccgtga cttttcgttc tgcctggaaa
     2401 ttgaagccgg cgaagactgt cgcagccaag gtcagcaaaa aaaggaacac tgcgacgaaa
     2461 cgagcttggc tttcccgatt catggacttg cgctctggcc ccgaaccccc gaagggctgg
     2521 agaggcttgg gagaggcatc ctgccggtcg tcaatgagca acttcgctgc catgtt
//
LOCUS       DASPUL010000273         5615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_594392, whole genome shotgun sequence.
ACCESSION   DASPUL010000273 DASPUL010000000
VERSION     DASPUL010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594392"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(374..1321)
                     /locus_tag="VEJ00_06725"
     CDS             complement(374..1321)
                     /locus_tag="VEJ00_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXX00890.1"
                     /translation="MKRLSFLISLTNDDNDYQQEQAAAAQTAARRLGIEVKIVHANND
                     ALTQSQQLLHYIQDSHVGRPDAIVFEPAGGTAFPQVARAAAAAGIGWVVLNHEADYVP
                     ELRRAYKVPIFAVSADHEEVGKIQGKQFATLLPNGGTILYIEGPPNSLPAKQRTQGMN
                     KTRPANIVVKTMRANWTEESSHRAVSSWLRLRTSLESHIDLVGAQDDSMAMGARKAFH
                     EIPEAERARWMKIPFTGCDGMPKHGQAWVRNGFLAATIFIRPNTDLAIEMLAEAFKTG
                     NLPVERKLTSPESVPDLANLAATASAHSAADKSHRSAGV"
     gene            complement(1443..1844)
                     /locus_tag="VEJ00_06730"
     CDS             complement(1443..1844)
                     /locus_tag="VEJ00_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00891.1"
                     /translation="MGEQPDSTAPEVSANESNVVDRPFRVKLRGSVLVLVRLPNRRSV
                     RAAFQQLSTTGGVIHLEKPLDEKIEVELIFHLGRTTIRGKAQMLFPMWATQGWMQPFR
                     FVDLSGDQRDTLDANLKSFLGDTANGTSAGA"
     gene            complement(1946..2416)
                     /locus_tag="VEJ00_06735"
     CDS             complement(1946..2416)
                     /locus_tag="VEJ00_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00892.1"
                     /translation="MEVLTAKTTRRSTRLRVEMPVSVISLDRTRPFGEQCTLLVVSAQ
                     GCGFRCSEALQLETPIMLSGLPGGGSVTARVANCLPLGNDGKYYLIGAALYTHGNVWR
                     IADPPADWSVVEPGAPAPGPAHGAPDNSSPLKVRKKSWPYNLFEERTEAHPGRK"
     gene            complement(2540..3355)
                     /locus_tag="VEJ00_06740"
     CDS             complement(2540..3355)
                     /locus_tag="VEJ00_06740"
                     /inference="COORDINATES: protein motif:HMM:NF026098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXX00893.1"
                     /translation="MVEGLLTKATVFLGGGRITGALAAGLRLAGDEREIVVYDRHPEK
                     VRALRRESRVITARDLGSAIARAEMMIVAVRPGSVREILAEVVACGAVPPKLCISLAA
                     GIPLRNLREWLGPPARWVRAMPSPVCRIGRGLTPLCFDRSVRKTERVRVRRMFAQVGP
                     VLELPERQLDAITAAHSPTHGYHAVATLARAAHDAGLDRETALTAAAHALGDGIAYWQ
                     QSGLSLEELLHEAATPGGIAAATMTAMDKSGYARSVASGVRAGIKQAQRNARR"
     gene            complement(3333..4094)
                     /locus_tag="VEJ00_06745"
     CDS             complement(3333..4094)
                     /locus_tag="VEJ00_06745"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXX00894.1"
                     /translation="MRLKDKVALITGGTSGIGEATALLFATEGAAVVITGRNTRRGAG
                     VVKRIRKGGGEGTFLRADVSRLKDCERSVRETLDTFGRIDILFNNAGVFYPQTAIECS
                     EGEWDEQIDVNLKGTFLMCKFILPAMIAQKSGVIINNSSGWGIVGGDHAVAYCASKGG
                     VVLMTKAMAIDHGAQGIRVNCICPGDVETPMLPADAKTRGLKWKDYMAGCSSRPLGRV
                     GTPEEIAKAVLFLACDDSSFMTGAALVVDGGGTAD"
     gene            complement(4170..4922)
                     /locus_tag="VEJ00_06750"
     CDS             complement(4170..4922)
                     /locus_tag="VEJ00_06750"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXX00895.1"
                     /translation="MRGLKGKRVLITGGASGIGAATAARFLEEGSRVCVLDRNEKACA
                     HVKRELPALGEAIIADVTDLMQVEAAFAEAIRLMDGVDILVNNAGISIRHKFLDITPE
                     EWERVIAVNLTGVFYVAQTAARHMWERGSGVILQTASTNGVMGYPYYADYNATKAGVI
                     ELTRSMALELAPKVRVCAVAPGYVLTPMQRAEYSDAMLEEVNRKIPLGRHASPEEIAG
                     LFAYLASDDAAFATGQVFTLDGGETAGGLASR"
     gene            complement(5053..>5615)
                     /locus_tag="VEJ00_06755"
     CDS             complement(5053..>5615)
                     /locus_tag="VEJ00_06755"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXX00896.1"
                     /translation="KEEFAAMKEGVCFLNLSRGFVVDHEALAVCLKSGKVAGAAVDVF
                     PKEPESGTGFKSVLQGLPNVILTPHIGGSTEEAQQNIGSFVSARLVDYVRTGNSMLSV
                     NLPHCHLEYEPGSHRLMHIHHNVPGILRAINDILADRGINIERQVLDTRGTLGYAIYD
                     INRPCDEELMRLLRAVPHTISVRTGC"
BASE COUNT         1077 a         1855 c         1516 g         1167 t
ORIGIN      
        1 gaagcgaaga gcacaaggcc atcccgtgat acttgcgctg cctcgtgatg ttgcttcgac
       61 cctgtaactg ccgccccact caggggactg tttcgggaat ctcatctcgc cgggtagatg
      121 gccagactgt tgaaacatcc ctattacgct caatccccca attggatacc agccggtgcg
      181 ctggtggtgg ctaaacgcgc agcgttcgcc gggctttcag ccacgtcaaa cttgtccaat
      241 tagacaagtc accttctatt ttgggaggtt tgctagtttt tatcctattg aaaacactgc
      301 aagttactga ttttgcagag gggctttaag ttccaaaggg acgcctgaaa cgttcatcac
      361 catcaagccc cgatcaaact ccagcggacc gatgagactt atcagccgcg gagtgtgcac
      421 tggccgtcgc cgccaagttc gccaggtctg ggactgactc cggactggtc agcttgcgct
      481 cgaccggcaa gtttcccgtt ttgaacgcct ccgccagcat ctcgatggca agatcagtgt
      541 tgggacgaat gaagattgtc gccgccagaa atccgttgcg cacccaagcc tggccgtgct
      601 tcggcatccc gtcgcatccc gtgaacggaa tcttcatcca gcgcgcgcgc tcggcttccg
      661 gaatctcatg gaacgctttt ctcgcgccca tggccatgct gtcatcctgg gcgcccacca
      721 gatcgatgtg gctttcgagg gaagttcgca ggcgcagcca ggaactcacc gccctgtgcg
      781 agctctcctc agtccagttc gcccgcatcg tcttcaccac gatattcgcc ggcctggttt
      841 tgttcatgcc ctgggttcgc tgtttcgccg gcaggctgtt cggcgggccc tcgatgtaca
      901 ggatcgttcc tccgttgggt agcagcgtcg cgaactgctt gccctggatc ttgccgacct
      961 cttcatggtc ggcgctcacc gcgaatatgg gaactttgta agcacggcgg agctccggga
     1021 cgtaatccgc ctcatgattc agcaccaccc atccaatacc cgctgcggct gctgctcgcg
     1081 ctacctgcgg gaatgcggtt ccgcccgccg gttcgaacac gatcgcgtcc ggtcgcccaa
     1141 cgtgtgaatc ctgtatgtag tgcaacagct gctggctctg ggtaagagcg tcattgttgg
     1201 cgtgaacgat ctttacttca atccccagac gccgcgctgc agtctgggcc gccgcagcct
     1261 gctcctgctg ataatcgtta tcgtcgttgg tgagtgaaat caggaagctg agtcgtttca
     1321 tcgcagacga tcctgtttgc gatgatagtc caactgtctt cgtcaccaag gtaaccagag
     1381 taaccgcggg cggtgcagtt ttggtcctgc gccaacgtgg gaatcaggtg ggaatgtagg
     1441 gttcaagcgc cggccgaagt accgttggcc gtgtcaccca gaaaggattt cagattcgcg
     1501 tccagcgtgt cccgctggtc gccggaaagg tccacaaagc ggaagggttg catccagccc
     1561 tgcgttgccc acatcggaaa cagcatctgg gctttgcccc ggatcgtagt ccggccgagg
     1621 tggaagatca gttccacttc gatcttctcg tccagcggct tttccagatg gattactccg
     1681 cccgtcgtcg acagctgttg aaacgctgct ctcaccgaac gccggttggg caaccgcacc
     1741 aacaccaaaa ccgacccgcg cagcttcacg cgaaacggcc ggtccaccac gttgctttcg
     1801 ttggccgaca cctcgggcgc ggtcgaatcg ggctgttcac ccatacatca cgagtatgac
     1861 agtggcgggt aaggcccacg aagttaccaa agaacaacga ccgagccttg tctttgtgag
     1921 ttgtgccggg aaagttggaa cgcgctcact tgcgcccagg atgggcttcc gttctctcct
     1981 cgaataggtt atacggccac gatttcttgc gcaccttcag aggagaggaa ttgtcgggag
     2041 cgccatgtgc cggacctggt gcaggcgctc cgggctcgac aacactccag tctgccggag
     2101 gatcggcaat cctccagaca tttccgtgcg tgtatagggc cgctccgatc aggtagtact
     2161 tgccatcgtt gccaagcgga aggcagttgg caacgcgcgc ggttacgcta cctcctccgg
     2221 gaagcccgct caacataatg ggcgtctcga gctgcagcgc ttcagaacag cgaaatccgc
     2281 atccctgggc acttaccacg agcagcgtgc attgctcgcc aaacgggcgc gtgcggtcaa
     2341 ggctgatcac gctgaccggc atctccacgc ggagccgggt gctgcgtcga gttgtctttg
     2401 ccgttagaac ttccatagga ggtgccgttc gcacgccgga tgtttcgccg tatccgatgt
     2461 gttcagtcgc accttatatt gtgacagcag ccacgtcgag ccgcatggtg acaaaggtaa
     2521 ccccgctcgt gggcaggtgt caccgacggg catttcgctg tgcttgtttg atgcccgctc
     2581 gaactccgct ggcaacggat cgcgcatatc cggatttgtc catcgccgtc atggtggctg
     2641 ccgcgatccc tcccggtgta gctgcctcat gtaagagctc ttccagggac aagccgcttt
     2701 gctgccagta tgcgatccca tcgccgaggg catgggccgc ggcggtcaag gcagtctcgc
     2761 gatcgagtcc cgcgtcgtgt gcggccctgg ccagcgtagc gactgcgtga tagccgtggg
     2821 tcggcgagtg cgccgcagta atcgcgtcga gttgacgttc aggcagctcc agcaccggcc
     2881 ccacttgtgc aaacatccgc cgcactcgca cgcgctccgt tttgcgaacg cttcggtcaa
     2941 agcacaacgg cgtcaaccct ctaccgatgc ggcacaccgg actgggcatg gcacgcaccc
     3001 agcgcgccgg aggccccagc cactcccgca gattgcgtag cggaatcccc gctgccaggc
     3061 tgatgcaaag ctttggcggc accgctccgc aagcgaccac ttcggcgagt atctcgcgca
     3121 ccgaacccgg tcgcactgcc acaatcatca tctccgcccg cgcgatcgca gacccgaggt
     3181 cgcgcgcagt aattactcgc gactctcgcc gcaacgcccg gactttctcg ggatgccgat
     3241 cgtaaacgac aatctcccgc tcatcgccag ccaaacgcaa ccccgcggcc aaagccccgg
     3301 taatgcgccc gccgcccaga aacacagttg ccttagtcag cagtccctcc accatccacg
     3361 accagcgccg caccggtcat gaacgacgaa tcatcacatg ccaggaacaa caccgctttg
     3421 gcaatctctt cgggtgttcc gactcgcccc aacggacggc tggagcatcc cgccatgtaa
     3481 tccttccact tcaggccgcg cgtcttcgca tcagccggaa gcatgggcgt ctcgacgtcc
     3541 cccgggcaaa tgcaattcac ccggatcccc tgcgccccgt gatcgattgc catagccttg
     3601 gtcatgagca cgacaccgcc cttcgacgcg cagtacgcca cggcgtggtc tccgcccacg
     3661 atgccccacc ccgagctgtt gttgatgatg actccgctct tctgcgcgat catcgccggc
     3721 aggataaact tgcacatcag aaatgtgccc ttcaggttga catcgatttg ctcatcccat
     3781 tctccctccg aacactcgat cgcggtctgc ggatagaaca cgccggcgtt gttgaacagg
     3841 atgtcgatcc tgccgaatgt gtcgagggtc tctcgaacgg accgctcaca gtctttcagc
     3901 cggctcacat ctgcgcgcag aaaggtcccc tcgccgccgc ctttcctgat tctcttcact
     3961 acgcccgcgc cgcgccgagt gtttctcccc gtgatcacca cggcagcacc ctcggtggcg
     4021 aacaggagcg ccgttgcctc gccgatgcca gaggtcccgc cggtaatcag tgcgactttg
     4081 tccttgagac gcatcgtgca ggaaccgtgg aatgatgaat gccgattggc gaatgaaatc
     4141 tgaaaacggg cagaagcatt tcagtttctc tatctactcg ccaatccccc cgccgtttct
     4201 cctccatcca gcgtaaacac ttgcccggtt gcaaacgccg cgtcatccga cgccagatac
     4261 gcaaacagcc cggcaatctc ctccggcgag gcgtgcctcc ccagcggaat tttccgattc
     4321 acttcctcaa gcatcgcatc agagtactcc gcgcgctgca tcggtgtcag aacgtatccc
     4381 ggagccaccg cgcagacgcg caccttgggc gccagttcca gcgccatcga ccgtgtcagc
     4441 tcaatcactc ccgccttggt cgcgttgtag tcggcatagt acggataccc catcaccccg
     4501 ttcgtcgacg ccgtctgcag aatcacgccg ctgccccgct cccacatatg ccgcgccgcc
     4561 gtctgcgcca cgtagaacac gccggtcagg ttcaccgcga tcacgcgctc ccattcttcg
     4621 ggcgtaatgt ccaggaactt gtggcgaatg ctgatgccgg cgttgttgac cagaatgtcg
     4681 accccgtcca tcagccggat cgcctcggca aaggcggctt cgacctgcat caggtcggtg
     4741 acgtcggcga taatcgcctc gcccaatgcc gggagctcgc gtttgacgtg cgcgcaggcc
     4801 ttctcgttgc gatccagcac acatacccgt gatccttctt ccagaaaccg cgcggccgtc
     4861 gccgcgccaa tgccgctggc tccgccggtg atcaaaacac gcttgccttt gagtccacgc
     4921 atgaaggctc cttagattat gtagccacga acgcattcgt ctgtggtcca agtgaagctc
     4981 ggatccagtc tcgcagcgtg cacgcctcgc ttcgcttcgg cggaacggac gaatgcgtcc
     5041 ttccctacac aatcagcatc ccgttcggac cgaaatcgta tgcggcaccg cacgcagcag
     5101 ccgcatcagt tcctcatcgc agggacgatt gatgtcatag atcgcatatc ccagcgtgcc
     5161 gcgcgtgtcc agcacttgcc gctcgatatt gatcccgcga tcggccagaa tatcgttaat
     5221 cgcccgcaaa atccccggca cgttgtgatg aatatgcatc aggcggtgcg accctggttc
     5281 gtactccagg tgacaatggg gcagattcac actcagcatc gaattaccag tacggacata
     5341 gtcgaccagc cgcgccgata caaacgagcc gatattctgc tgtgcttctt cggttgagcc
     5401 cccaatatgc ggcgtcagga tcacattcgg cagcccctgc aacacactct tgaagcctgt
     5461 cccggattcc ggttcctttg gaaacacgtc taccgcggca cccgcgacct tgccgctctt
     5521 caggcacaca gccagcgctt catgatccac gacgaacccg cggctcagat tcagaaagca
     5581 caccccctct ttcattgcgg cgaattcttc tttgc
//
LOCUS       DASPUL010000274         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1412619, whole genome shotgun sequence.
ACCESSION   DASPUL010000274 DASPUL010000000
VERSION     DASPUL010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412619"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1441
                     /locus_tag="VEJ00_06760"
     CDS             137..1441
                     /locus_tag="VEJ00_06760"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HXX00897.1"
                     /translation="MADIRPFRALRYNLRQVSATEVVTQPYDKITPGMRERYYAASPY
                     NLVIIILGRAESGDNTAQNVYTRAAAFSRQWRTEGIFTQDSSPSVYTYSQTFTAPSGS
                     TFERRSFIALGRVEDYSAKVVFRHEQTLAKPKADRLDLLRATRVHYEQLFLLYEDAGE
                     IDALLVPDPNMRPIIDVADEYGVAHRVWQISDPVVTSSVQEKMRDKKLVIADGHHRYE
                     TALNFRNECRGKTTAGSNPDAPYEFVMMTFVNMNDPGLLILPTHRVVHSLDSFSADDF
                     QNSARQCFEIEQTKPALDAACATSLLRERGRTGMALLAVTRDCAFVLHSPKPAAAQFL
                     AGLSARQQSLDVVVLHKCLLEGVLKLSEESIRNQQNLSYVREAKEALDVVRNGSANIA
                     FLMNPCRVQQVRDIAFAGEVMPQKSTDFYPKLLSGLTAYALD"
     gene            1620..>2307
                     /locus_tag="VEJ00_06765"
     CDS             1620..>2307
                     /locus_tag="VEJ00_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00898.1"
                     /translation="MYSVAANYSVTLTQRDGRDYVGLLEVLEPLGKVSARVDGARWRL
                     RYYRVEGTFQAGRGRARIGGYDIDLPAKFLLENDRGLVPVSALSVLLPRFLGGPVTLH
                     EDAGRLFIGSVATHFTASLAAQNPPRLVFNFTAPVNPTVATEPGFVRMIFTREPVVAP
                     ASPTLTFGSKAVPSATYTEGNGTAVVTVNSTTALMATFSNDGRTITLSPPSVAAQTAA
                     QSPSAASAPGA"
BASE COUNT          450 a          728 c          635 g          494 t
ORIGIN      
        1 gccgggcggc gcagcggaat tgtcaaaaat gacggacttt tccactcagg cactcctgca
       61 ccgccagtgt tgcacatcgc ccgtgtggcg cgcgtcccag cgcgtgtcgc tatcttgtta
      121 caatcaaagg tttcctatgg ccgacattcg tccttttcgc gctcttcgct acaatctgcg
      181 gcaggtttcg gcgacggagg tagttaccca accctacgac aaaatcactc cgggcatgcg
      241 ggagcgctac tacgctgcca gcccgtataa cctcgtcata atcattctcg ggcgcgccga
      301 atccggggac aataccgcac aaaacgtata tacacgggcc gctgctttct cgcggcaatg
      361 gcggaccgag ggtatcttta cgcaagattc ctcaccttca gtttatacgt attcgcagac
      421 gttcaccgcc ccgtcgggca gcacttttga gcgccgatcg ttcattgccc tcggccgcgt
      481 ggaagactat tccgccaaag tggtctttcg ccacgagcag accctggcca agccgaaagc
      541 agaccgcctc gacctgctgc gcgccacgcg ggttcactat gagcagctat tcttactcta
      601 cgaagatgcc ggcgagattg atgccctgct cgttcccgat ccaaatatgc ggcccatcat
      661 cgatgtggcc gacgaatacg gagttgcgca ccgcgtctgg cagatttccg acccggtagt
      721 gacgtcttct gttcaggaaa agatgcgcga caagaagctg gtcattgccg acggccacca
      781 ccgctacgag actgcgctta attttcggaa cgaatgccgg gggaagacta cggcaggctc
      841 caaccctgac gctccctacg aatttgtcat gatgacgttc gtcaacatga acgatccggg
      901 gcttctgatc ctgccaacgc accgggttgt gcactcgctg gactctttct cggcagacga
      961 ttttcaaaat tcggctcgac aatgctttga aatcgaacag accaagccgg cgctggatgc
     1021 cgcctgcgct acatccctgc tgcgcgagcg tgggcgtacg ggaatggcac tgctggccgt
     1081 cactcgcgac tgcgcgtttg tgctgcactc tcccaaacca gccgcggcgc agttccttgc
     1141 gggactgtcg gcacgtcagc aatcactgga tgtcgtcgtg ctgcacaaat gtcttctcga
     1201 aggagttctg aagctctcgg aagagtccat ccgcaaccag cagaatctct cctatgtgcg
     1261 cgaggccaag gaggctttgg acgtggtgcg caacggcagc gccaatattg cattcctcat
     1321 gaatccctgc cgcgtgcagc aggttcgtga catcgccttt gccggcgaag tcatgccgca
     1381 gaagtccacc gatttctacc ccaaactgct gagcggactg accgcctatg cgctggacta
     1441 atcttgattt cagattttcg atttcagatt gcagatttct tgcggaaggg cttcgggcat
     1501 tcaatctgca ctctgcaatc gcaaatcgaa aatccccctt taacgctgtc accgccggcg
     1561 ttctgacctt catttgtaca ctggcgctta cggctgctgc ccccgaaaag cgcctctcgg
     1621 tgtactcggt cgcggccaac tactcggtca ccctgacgca acgcgacggc cgcgactacg
     1681 ttgggttgct cgaagttctc gagcccctcg gcaaggtaag cgctcgcgtg gacggtgcgc
     1741 gctggcgttt gcgctattac cgggtggaag gaacatttca ggccggccgg gggcgcgccc
     1801 gcatcggagg atacgatatc gatcttccgg cgaagttcct tctggagaac gaccgcggac
     1861 tcgtaccggt ctcggcgctc agcgtcctgt tgcctcgctt cctcggcgga ccggtcactc
     1921 tgcacgagga tgcaggccga ctgttcatcg gcagcgtggc cacacacttc acggcatcgc
     1981 tggcagcgca gaaccctccg cggctggtct tcaatttcac tgcgcccgtg aatcccaccg
     2041 tcgcgacaga accgggtttt gtgcgcatga tattcacgcg cgagcccgtg gtcgctccgg
     2101 cctcgccgac tctcaccttt ggcagcaaag cggttccttc cgccacttat acagaaggaa
     2161 atggaacggc agtggtaacg gtgaactcga caaccgcatt gatggcgacg ttcagcaacg
     2221 acggccgcac gattacgctg tcaccgccct ctgtggctgc gcaaaccgca gctcagtcgc
     2281 cgtcggcagc atctgcgccg ggcgcgt
//
LOCUS       DASPUL010000275         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1691483, whole genome shotgun sequence.
ACCESSION   DASPUL010000275 DASPUL010000000
VERSION     DASPUL010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691483"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2017
                     /locus_tag="VEJ00_06770"
     CDS             <1..2017
                     /locus_tag="VEJ00_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXX00899.1"
                     /translation="ASTTILRQQLKWVTRGTVLAVAPYTLFYVLSYVFPHAMAWLPEG
                     AMKLSVLSLGLLPLTFGYAIFRYRLMDVDLIFKRGVVYTLAAATVAGLYFALVAGVAW
                     LVQMQKPATGAVGLILAVVVTALLFDPVRKWIQDRVDQFFYRTRYDYRRTLLEFGREL
                     SSETDLNALLSSALDRLSRTLAVDRLAIFLNTGEEPERFVLSKSAGIVAPPQLNLSFL
                     TVPRPEQAGHLFFENTHQVPRETEGARQAIARLDLNYYIPCRAQQKTVAVLGLGKTVK
                     GDFLSSEDVELLETLGGYLGIAIQNGRLYASLQQKVAEYERLKDFNENIVESINVGVM
                     ALDMEDRIESWNAQMEVMYALPRWQALTQPVPTVFPAEFVEEFYRVRQNPGISNLYKF
                     RMRTPAGETRTVNVAIAPLVTRKFQVIGRLVIMDDITARVELEGQLSQADKLSSIGLL
                     AAGVAHEVNTPLAVISSYTQMLAKQLQGDPQKSGLLEKITRQTFRASEIVNNLLNFSR
                     TSGTEFADIDINKVITDTLALLEHQFKTAKIQVQSELTPAISPIQGNAGRLQQVFLNL
                     FLNAKDAMPAGGTLSVATANGELVSVRVSDTGSGIAPEHIQRIYDPFFTTKTSGKEGQ
                     NRGTGLGLSVTYGIIQEHAGKIRVESHPGAGTTFALDFPLSRKVVHV"
     gene            2010..>3008
                     /locus_tag="VEJ00_06775"
     CDS             2010..>3008
                     /locus_tag="VEJ00_06775"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXX00900.1"
                     /translation="MSEAAVISRGMLHGDPLPAGSVLIIDDEAEIRESLQTLLELEGF
                     EVESAGTGEDGMARIGDRPFDLVLLDLALPGADGIEILSEIRAHDPNLPVIMITAYGT
                     VENAVRAMQSGAANFVQKPWDSEKLLADVRAAVGRRRAEEENVQLKRALKQRYNFTNI
                     VGKSEPMLKIFDLVGQVASSRSTVLLQGESGTGKEVIAKAIHLNSPRRDKAFVPVNTG
                     SMPADLLESTLFGHVKGAFTSAVASKKGLFEVADKGTLFLDEIATMGLETQAKILRVL
                     QDRRFMHLGGIQEIQVDVRIIAATNVDLRQQVREGRFREDLFYRLNVITIELPPLRQ"
BASE COUNT          589 a          887 c          968 g          564 t
ORIGIN      
        1 ggcgagtacg accatcctgc ggcagcagtt gaagtgggtc acgcgcggga cggttcttgc
       61 ggtcgctcct tacacgctct tctatgtgtt gtcctacgtc tttccgcacg ccatggcgtg
      121 gctgccggaa ggcgccatga agctgtcggt attgtcgttg gggcttctgc cgctgacctt
      181 cggctacgcg attttccgct accggctgat ggacgtggac ctgatcttca agcgcggtgt
      241 ggtttacacg ctcgccgccg cgaccgtcgc cggactgtac ttcgctctgg tagcaggcgt
      301 ggcctggctg gtgcagatgc agaagccggc caccggcgcc gtgggattga ttctggcggt
      361 ggtggtgacg gcgcttttgt tcgatccggt gcgcaaatgg attcaggatc gcgtcgacca
      421 gttcttctat cgcacgcgct acgactaccg gcgcacgctg ctcgagtttg gccgagaact
      481 cagttcggaa acggatttga acgcgttgct ttcttctgcg ctcgaccggc tgtcgcgcac
      541 tctcgccgtg gaccggcttg ccatctttct gaacacgggc gaggagccgg agcggttcgt
      601 gctttcgaaa tcggcgggca ttgtggcgcc gccgcagctc aatctgagct ttctgactgt
      661 gccgcggccc gagcaggcgg gacatctgtt cttcgagaac acacaccagg tgccgcgcga
      721 aaccgagggg gcgcggcagg cgatcgcgcg cctggatctg aactactaca tcccgtgccg
      781 cgcgcagcag aagacggtgg cagtcctcgg gctgggcaag actgtcaagg gcgacttcct
      841 ttcgagcgaa gacgttgagc tgctggagac actgggcggg tatctgggca tcgccatcca
      901 aaatggccgt ctgtatgcct cgttgcagca gaaggtggcc gagtatgagc gcctgaagga
      961 cttcaacgag aacatcgtgg agtcgatcaa cgtgggcgtg atggcgctcg atatggaaga
     1021 ccgcatcgag agctggaacg cgcagatgga agtcatgtac gcgcttccgc gatggcaggc
     1081 ccttacgcaa ccggtgccga cggtcttccc ggcggagttt gtcgaggagt tctatcgcgt
     1141 ccggcagaac ccgggcatca gcaatctgta caaattccgc atgaggacgc cggcgggcga
     1201 gacgcgcacg gtgaatgtgg ccatcgcgcc gctggtgacg cgtaagttcc aggtgatcgg
     1261 acgactcgtc atcatggacg acatcacggc gcgcgtggag cttgagggcc agctttcaca
     1321 ggcggacaag ctgtcgtcga tcgggcttct ggcggctggg gtggcgcatg aagtgaacac
     1381 gcccctggcg gtgatctcct cgtacacgca gatgctcgcg aagcaactgc agggcgatcc
     1441 gcaaaagtca ggcctgctcg agaaaatcac ccggcagacc ttccgagctt cggagattgt
     1501 caacaacctg ctgaatttct cgcggacgag cggaaccgag tttgccgaca ttgatatcaa
     1561 caaggtcatc accgacacgc tggctctgct ggagcaccag ttcaaaaccg ccaagatcca
     1621 ggtgcagtcg gaactgactc cggcgatctc tccgattcag gggaacgccg gccggctgca
     1681 acaggtcttc ctgaatctgt ttctcaacgc caaagacgcg atgcccgcag gcggcacgct
     1741 cagcgtggcc acggccaacg gagaactggt cagcgtgcgc gtttctgaca cgggatcggg
     1801 tatcgcgccc gagcacatcc agcgcattta cgatcccttc tttacaacaa agacgtcagg
     1861 caaagagggc caaaatcgcg gaacggggct aggcctctcc gtgacctacg gaatcatcca
     1921 agaacatgca ggcaagattc gcgtcgaaag ccatcccggc gcgggaacga cctttgccct
     1981 ggattttccg ctgagcagga aggtggtcca tgtctgaagc ggcagtgatt tcgcgcggca
     2041 tgctgcacgg ggatcccctg ccggcgggct cggtgctcat tattgacgac gaggccgaga
     2101 ttcgcgaatc gctccagacc ctcctcgagc tggaaggatt tgaggttgag agcgcgggca
     2161 ctggcgaaga cggcatggcg cgaatcgggg atcgcccgtt cgatctggtc ctgctcgacc
     2221 tggcgctgcc gggggccgac ggaattgaga ttctctccga aatccgggcg cacgacccga
     2281 atctgccggt gatcatgatt accgcctacg gcacggtgga gaacgcggtg cgcgccatgc
     2341 aatcgggcgc cgccaatttc gtccagaagc cgtgggacag cgagaaactg ctggcggatg
     2401 tgcgcgccgc cgtgggacgg cggcgcgcgg aagaagaaaa cgtccagctc aagcgcgcgc
     2461 tcaaacagcg ctacaacttc acgaacattg tcggcaaaag cgagcccatg ctgaagattt
     2521 tcgacctggt ggggcaggtc gcgagcagcc gctcgaccgt gttgctgcag ggcgaaagcg
     2581 ggacgggcaa ggaagtgatc gcgaaagcga tccacctgaa ctcaccgcgg cgcgacaagg
     2641 cattcgtccc ggttaatacc ggctcgatgc ccgccgatct gctcgaatcc acgctctttg
     2701 gccacgtcaa gggagcgttc accagcgccg ttgcatcgaa gaaaggtctg ttcgaggtcg
     2761 cggacaaagg cacgctgttt ctcgacgaaa tcgccaccat gggcctggag acgcaggcca
     2821 agatcctgcg cgtgctgcag gaccggcgct tcatgcacct gggaggcatc caggagattc
     2881 aggtggacgt gcgcatcatc gccgctacta acgtggatct gcgccagcag gtgcgcgaag
     2941 ggcgcttccg cgaagacctg ttttaccggc tgaacgtcat caccatcgaa ctgccgccgc
     3001 tgcgccag
//
LOCUS       DASPUL010000276         5300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_730552, whole genome shotgun sequence.
ACCESSION   DASPUL010000276 DASPUL010000000
VERSION     DASPUL010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730552"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..74)
                     /gene="rpmJ"
                     /locus_tag="VEJ00_06780"
     CDS             complement(<1..74)
                     /gene="rpmJ"
                     /locus_tag="VEJ00_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXX00901.1"
                     /translation="MKVRASVKKICDKCKIIRRRGVVRV"
     gene            complement(147..365)
                     /gene="infA"
                     /locus_tag="VEJ00_06785"
     CDS             complement(147..365)
                     /gene="infA"
                     /locus_tag="VEJ00_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXX00902.1"
                     /translation="MSKEDAIEVMAVVLEPLPNAMFRVELENKHQVLAHVSGRMRKNF
                     IRILPGDKVAVELSPYDLTRGRIVYRYK"
     gene            complement(440..1192)
                     /gene="map"
                     /locus_tag="VEJ00_06790"
     CDS             complement(440..1192)
                     /gene="map"
                     /locus_tag="VEJ00_06790"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXX00903.1"
                     /translation="MAIVCKSAAEIEKMRHSGRIVRQVLDHVKPLVIPGATTMDLERA
                     AEKRIKELGAKPAFKGYYDYPCVLCTSVNEEIVHGIPSEKRVLKEGDIVSIDCGVVLD
                     GYYGDAAITVPVGMSLKPELQKLLEVTEASLYRGIEQARVGNSVGDVGAAVQEHVEAA
                     GFSVVREFVGHGIGTKLHEEPQVPNFGSRGHGAKLREGMVLAIEPMVNFGKPETRVLG
                     DKWTAVTADGSCSAHFEHCVAVTKDGPVILTQ"
     gene            complement(1208..1927)
                     /locus_tag="VEJ00_06795"
     CDS             complement(1208..1927)
                     /locus_tag="VEJ00_06795"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HXX00904.1"
                     /translation="MAQETSRDAALLNVGPVVLLGPPGAGKGTQAKRIMEHYGIPQVS
                     TGDLLRENVAKGTDVGLAAKVVMARGELVSDDLVCEMVRERLKRPDCKRGYILDGFPR
                     TAAQAGWLDALLDHELFDNSRPTRAWPIVICLDVDYNQLLLRLTGRRSCPTCGRIYNV
                     HFQPPREDEICDESHATKLMTRNDDRLEVIQPRLTAYQEQTRPVADYYERTRRLISVN
                     GDLPIDEVTAQIYRIIEDHHA"
     gene            complement(2040..3425)
                     /gene="secY"
                     /locus_tag="VEJ00_06800"
     CDS             complement(2040..3425)
                     /gene="secY"
                     /locus_tag="VEJ00_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXX00905.1"
                     /translation="MFDKLANIFRIPDLRRRILFTLGLLFVYRVGGHLPTPGIDTAKL
                     ESFFTQSGTSGSLLGFVDLFSGGQLRRMTIFALGIMPYITASIILQLLTVVYEPLAKL
                     QKEGELGRKKITQWTRYITVMLSAVQSFGIAIGLEKGGFALTPGWGFRLMTMLTLTTG
                     SAFIMWLGEQITERGVGNGMSLLIFAGIVVGLPRGVVDLVEKAKNATWGAITFPLMVG
                     LIIFMIAVVAFIVYVERSERRIPVQYAKRVVGRKVMGGQSTHLPLRVNAGGVMPVIFA
                     SSILTMPQTIGYGLRTNRYFGDMIRWLGWGEPLYTLLYAVGIIFFAYFYVSIVFNPNE
                     VADNMRKYGGFIPGIRPGKRTADFINEVLTRITLVGALYLIIISFIPEWMITGIHLNH
                     LYGPIGRFFENLPNWVTNGLGVNFYFGGTSLLIVVGVAMDTVTQIEAQLIMRHYDGFT
                     PRSGRIRGRRW"
     gene            complement(3425..3877)
                     /gene="rplO"
                     /locus_tag="VEJ00_06805"
     CDS             complement(3425..3877)
                     /gene="rplO"
                     /locus_tag="VEJ00_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HXX00906.1"
                     /translation="MNLSNIHRPKKATESRKRVGRGMGSGMGKTSTRGHKGQRSRSGS
                     RMIRGFEGGQMPLHRRLPKRGFTNIFRKEYAIVSLERLAAIGETTITPEVLRKAGVVK
                     TKLPVKILGDGELKTALTVSAHKFSKSAQEKITKAGGKVEVLAVKAGQ"
     gene            complement(3894..4094)
                     /gene="rpmD"
                     /locus_tag="VEJ00_06810"
     CDS             complement(3894..4094)
                     /gene="rpmD"
                     /locus_tag="VEJ00_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HXX00907.1"
                     /translation="MTPKANSGGGKIHLKWVRSAICAPEKQKLVIKGLGFTRLHQVIE
                     RPDTAAIRGMVAKVPHLVEVVD"
     gene            complement(4192..4689)
                     /gene="rpsE"
                     /locus_tag="VEJ00_06815"
     CDS             complement(4192..4689)
                     /gene="rpsE"
                     /locus_tag="VEJ00_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXX00908.1"
                     /translation="MKKLDAGAFQLKDQVVAINRVTKVVKGGKNLSFAALVVVGDPSA
                     AVVGYGSGKAKEVPQAIRKGIESAKKNLVKVNLTQTSIPHLVLGRYGSGKVLLKPAPE
                     GTGVIAGGAVRAVMTSAGVQNVLTKSIGTTNPHNVIKATFDALKQLKNKESVATLRGK
                     VAEEL"
     gene            complement(4788..5153)
                     /gene="rplR"
                     /locus_tag="VEJ00_06820"
     CDS             complement(4788..5153)
                     /gene="rplR"
                     /locus_tag="VEJ00_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXX00909.1"
                     /translation="MLTKIGKNQKRGHIHERIRKKLRGTTERPRLNVYRSLNHIYAQV
                     IDDLSGKTLVAASTAEGKKEDRRTGGNVAAAKAVGKSIAERAKAKGVTKVVFDRGGYI
                     YHGRVKALADAAREAGLQF"
BASE COUNT         1035 a         1717 c         1440 g         1108 t
ORIGIN      
        1 acccgcacca ccccgcggcg gcggatgatc ttgcacttat cgcaaatttt cttgaccgat
       61 gctcgtactt tcataggaac ccagcaatta gcatttggca attagcattt ggccaattgt
      121 taagtgccaa gtgctatccc aaaccgtcat ttatatcgat aaactatccg tccacgcgtc
      181 aggtcgtacg gcgaaagctc gaccgcaacc ttatcgcccg gcagaatgcg tataaagttc
      241 ttgcgcattc ttccggaaac gtgcgccagc acctgatgct tgttctccag ttccacccgg
      301 aacatggcgt tgggcagcgg ttccagaacc acggccatta cctctatagc gtcttctttg
      361 ctcatccgct ccttagcttt tggctcttag ctcttggctc tcagccgaca gccaaaagcc
      421 aacgggccga aagcgtgctt tactgcgtca aaatcaccgg cccgtccttc gtcacggcca
      481 cgcagtgctc gaagtgggcg ctgcaactgc cgtcggcggt cacagcggtc cacttgtcgc
      541 cgagcacgcg ggtttccggc ttgccgaagt tcaccatggg ctcgatcgcc agcaccatgc
      601 cctcgcgaag cttcgcaccg tgtccgcgac tgccgaagtt cggaacctgc ggttcctcat
      661 gcagctttgt gccgatgccg tgtccgacga actcgcgcac cacgctgaat ccagcagcct
      721 cgacgtgctc ctgcacggcc gctcctacgt cgccgaccga gttgccgacg cgcgcttgct
      781 cgattccccg gtacaacgag gcttcagtga cttcgagcag cttctgcagt tccggcttca
      841 ggctcatgcc gaccggcacc gtaattgcgg cgtcgccata gtagccgtca agcaccacgc
      901 cgcaatcgat ggaaacgatg tcgccttcct tcagcacccg cttttccgac gggatgccgt
      961 gcacaatctc ttcatttacc gacgtgcaca agacgcaggg ataatcgtag taccccttga
     1021 acgccggctt cgcgccgagt tccttaatcc ttttctcggc ggcacgctcc aggtccatcg
     1081 tcgtggcccc gggaatcacg agcggcttca catggtccag aacctggcga acgatgcgac
     1141 cgctgtgtcg catcttctcg atctcagccg ccgatttaca gacaatcgcc atgacgtctt
     1201 ctgcacatca cgcgtgatga tcctcgatga tccggtaaat ctgcgcggtg acttcgtcga
     1261 tcggcaaatc gccgttcacc gagatcaatc gccgggtcct ctcatagtaa tccgccacag
     1321 gacgcgtctg ctcctggtaa gccgtcaaac gcggctgaat gacttccaat cggtcgtcat
     1381 tgcgggtcat cagcttcgtc gcgtgcgatt catcgcagat ttcgtcctcg cggggaggct
     1441 ggaagtgaac gttgtagatc cgtccacagg tgggacaaga gcgacgcccg gtaagccgaa
     1501 gcaagagctg attatagtcg acgtccaggc agatcacaat cggccacgcc cgcgtcggac
     1561 gcgaattgtc aaagagctca tggtcgagca acgcatcgag ccacccggcc tgggccgcag
     1621 tccggggaaa gccgtcgaga atgtagcccc gcttgcaatc cgggcgcttt agccgttccc
     1681 ggaccatctc gcaaaccagg tcgtcggaaa ccaactcccc gcgagccatt accactttgg
     1741 cggcgaggcc gacatccgtt cccttggcca cattctcccg cagcaagtct ccagtcgaga
     1801 cctgcgggat tccatagtgt tccatgatgc gcttggcctg agtgccttta cccgcacccg
     1861 gcggtcccag cagcaccacg ggtccgacat tgagcaaagc agcatcacgc gaggtttcct
     1921 gcgccatcgg cctggccaaa gcctcccgat atcagtttcc ccggcgcccc agttaacccg
     1981 attcgcccaa cccggccgag gtgcagcaac cccacaacgt gcaaaatctc tgccgcgagc
     2041 taccaacggc ggccgcggat acgtccgctg cgcggcgtga atccgtcata gtggcgcatg
     2101 atcaattgcg cctcgatctg cgtcaccgta tccatggcga cgccgacgac aatcaacaag
     2161 gaagttccgc cgaaatagaa gttcacccct agcccgttgg tcacccagtt cggcaagttt
     2221 tcgaagaatc ttccgatcgg gccgtacaaa tggttgaggt gaatgccggt gatcatccat
     2281 tcgggaatga acgaaatgat aatgagatac agtgcgccca ccagcgtaat ccgcgtgagc
     2341 acttcgttga tgaagtccgc cgtgcgcttc cccggacgaa tgccggggat gaacccgccg
     2401 tacttgcgca tattgtccgc gacttcattg ggattgaaca cgatggaaac gtagaagtag
     2461 gcgaagaaaa taatccccac cgcgtacagc agggtgtaca gcggctcacc ccatcccagc
     2521 cagcggatca tgtcgccaaa atagcgattg gtgcgtaagc cgtagccaat cgtctgcggc
     2581 atcgtgagga tggaagatgc gaagatgacc ggcatgacgc cgcccgcatt cacacgcagc
     2641 ggcaggtggg tggattgccc gcccatcact ttgcgaccga cgacgcgctt ggcgtattgc
     2701 acaggaatac ggcgctcgct gcgttccacg tagacgatga acgccaccac ggcgatcatg
     2761 aagatgatca ggccgaccat cagcgggaag gtaatcgctc cccaggtcgc gttcttggct
     2821 ttctccacca gatcgaccac gccgcgaggc aagccaacca cgatgccggc gaagatcaac
     2881 agcgacattc cgttgcccac gccgcgctcg gtgatctgct cgcccagcca catgatgaag
     2941 gcgctgcccg tggtcagggt cagcatggtc ataaggcgga atccccagcc gggagtcagg
     3001 gcaaaaccgc ccttttcaag cccgatggcg ataccgaacg actgcaccgc actgagcatg
     3061 acggtgatgt agcgcgtcca ctgggtgatc ttcttgcgtc ccagttctcc ttctttctgc
     3121 agcttggcca gcggttcata gaccacggtc agcaactgca gaatgatgga cgccgtgatg
     3181 tacggcatga tgccaagagc gaaaatcgtc atgcggcgca actggccgcc ggagaacagg
     3241 tcaacaaacc cgagcagcga gccgctggtt ccgctctggg tgaaaaagct ctcgagcttc
     3301 gccgtgtcga tgcccggcgt gggcagatgt ccgccgacgc gatacacgaa cagcagcccg
     3361 agggtgaaca gaatccgcct gcgcagatcg ggaatgcgga aaatgttggc cagtttgtcg
     3421 aacattactg ccccgccttc actgctaaca cctcaacctt gccgcccgcc ttggtgatct
     3481 tttcctgcgc tgatttggag aacttgtgcg cactcacggt gagcgcggtc ttcaattcgc
     3541 cgtcacccag aatcttgacc ggaagcttcg tcttgaccac cccagccttg cgcagcacct
     3601 caggcgtgat agtggtttcg ccgattgctg ccaagcgctc caagctaacg atcgcatatt
     3661 ccttccggaa aatgttggtg aagccgcgct tgggcaagcg gcggtgcaga ggcatctggc
     3721 cgccctcaaa accgcggatc atacgcgagc cgctgcgcga acgctgtccc ttgtggccgc
     3781 gagtggacgt cttgcccatg cccgagccca tgccccggcc cacgcgcttg cggctttccg
     3841 tcgccttttt tggtcggtga atgttggata gattcataaa tgcactctcg gccctagtcc
     3901 acaacctcta ccaaatgcgg caccttggcc accatgccac ggatcgcggc cgtgtcgggg
     3961 cgctcgatca cctggtgcag ccgggtgaag ccgaggccct tgatgaccag cttctgtttt
     4021 tcgggggcgc aaatggcgga gcgcacccac ttcaggtgaa tcttcccgcc gcccgaattt
     4081 gcctttggtg tcatttcaaa acctcagtgg tcagtggcca gtgatcagtg accagtaacc
     4141 gaattgtatc tacactggtg ctggcccctg acaactgacc ggccacaacg cttaaagctc
     4201 ctctgccacc ttgccgcgca aggtcgcgac actttctttg ttcttcaact gcttcaaagc
     4261 gtcgaaggtc gccttgatca cgttgtgcgg gttggtcgtg ccgatggact tggtgagcac
     4321 gttctgcacg cccgccgagg tcatcacggc gcgcaccgcg ccgccggcga ttacgcccgt
     4381 cccttccgga gccggcttca gcagcacctt gcccgagccg tagcggccga gcacgaggtg
     4441 cggaatgctg gtctgggtca ggttgacctt caccagattc ttcttcgccg actcgattcc
     4501 cttgcggatg gcctgcggaa cttccttggc cttgccggaa ccgtagccca ccaccgcggc
     4561 cgacgggtcg cctaccacca ccagcgcagc aaaggaaaga ttcttgccac ccttgacgac
     4621 cttggtgacg cggttgatag cgaccacctg atctttcaac tgaaaagcgc ctgcatcgag
     4681 tttcttgatt actgttggca ttcgtaaacc cagctttcag ctatccgctt tcagccgtca
     4741 gcttgccgct ccagccgata gctgagagcc gacagctgag agctgcccta aaattgcaat
     4801 cccgcttccc gggcggcgtc ggccagagcc ttcacccgcc cgtgataaat gtagccgccg
     4861 cggtcgaaca cgaccttggt tacacctttg gccttggcgc gctcggcgat tgacttgccg
     4921 acggctttcg ccgctgccac gttcccgccg gtgcgccggt cttccttctt gccctccgcg
     4981 gtgctggccg cgaccagcgt cttgccgctc aggtcgtcga tcacctgggc gtagatgtga
     5041 ttcagcgagc ggtatacatt caaccgcggg cgctcggtcg tcccgcgcag cttcttgcgg
     5101 atgcgctcgt gaatgtggcc gcgcttctgg ttcttgccga tctttgtaag cattgctgcc
     5161 ttgtccttct ctacaagcga gagatccttc gcttcgctcg ggatgaccgc ggcggcctca
     5221 gacgcccgcc aaacgcgatc actttgctcc tgtctttcca accttcttct tcaggcgctc
     5281 gcccacgtag cgcacgccct
//
LOCUS       DASPUL010000277         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1826383, whole genome shotgun sequence.
ACCESSION   DASPUL010000277 DASPUL010000000
VERSION     DASPUL010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826383"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VEJ00_06825"
     CDS             <1..1011
                     /locus_tag="VEJ00_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HXX00910.1"
                     /translation="FGGIPGSLPHLQIPRFRSDLIHGLLGPAFTVAMLGAIESLMSAV
                     VSDRMSNDRHNPNVELVAQGVANVFSPMFGGLPATGAIARTATSIRSGAQSPVAGMIH
                     ALTLLCILVFAAPLVSYVPMAALAAILMIVAYNMGEWREIPQLLKLTKTDISIWLVTF
                     ALTVFADLTVAVEAGMILAALLFISRVAATTTVSQVTEEYVEDGRVHILQDKDIPYYA
                     TIFRIHGPFLFGATDKIAAVTENLHRLPPVVILRLRNMTALDASGLFAIEEVAKQLHA
                     SKRTLILCGAREQPRKLMHQAEFEEVVGTDNICDNVQEALRRAEEVYEGLEANSATLG
                     RR"
     gene            complement(1052..2467)
                     /locus_tag="VEJ00_06830"
     CDS             complement(1052..2467)
                     /locus_tag="VEJ00_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXX00911.1"
                     /translation="MEAISSAAKPHLSLWTSLVEAVRGSHQDYTAGSLNRAIFLLAVP
                     MVLEMALESLFAVVDVFWVGRLGASAVAAVGLTESMLYIVFSVGLGLSLSTTAMVARR
                     IGEKDHEGAAVAAVQAIAIGLVVSFVIGLPCLILAPRLLQWMGASPEIVSVGSGYTRI
                     CLGGSWVVLLLFLNNAIFRGAGDAAIAMRLLWVSNIINLILDPCLIFGLGPFPRLGVT
                     GAALATFIGRGVGVMYQFYRLLKGTERIRVLVRQIRLQVDVLWRLVRVSLTGILQFAI
                     AHTSWIGLVRIVSIFGASALAGYTIAMRIVTFVILPSWGLSNAAATLVGQNLGAGEPE
                     RAETAVWRTGLYNMMFLGGVGVFFVLYATPAVRLFTHDPGVVTLGAACLRIVSYGNIG
                     YAYGMVMLQAYNGAGDTVTPTIVNFFGFWLLEIPLAYSLAILLRMESNGVFWAIVIAE
                     GSIAAASAFLFRQGRWKEQKI"
     gene            complement(2854..>3267)
                     /locus_tag="VEJ00_06835"
     CDS             complement(2854..>3267)
                     /locus_tag="VEJ00_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00912.1"
                     /translation="KNTAFGAPYVESDECWIGKIDHKDPDKVEWAKLPPHPGPAHFGI
                     VAGARGSKVIFSGGSPAVHSFKGLDHEGKPVELSPVTFDFDVHGNRWETLDEETYDPR
                     SDTRGMLSTPVGAVILGGMVSNQTATARVFLLPKK"
BASE COUNT          728 a          961 c          977 g          601 t
ORIGIN      
        1 ttcgggggaa ttcccggcag cttgccgcac ctgcaaatac ccaggttccg ctccgatctg
       61 attcatggcc tgttgggacc agcttttacg gtggcgatgc tgggggcaat cgaatcgctc
      121 atgtcggccg tggtgtcgga ccgcatgagc aacgaccgac acaatcccaa cgtcgagttg
      181 gtggcgcaag gggtggccaa cgttttttcg ccaatgttcg gcggtttacc ggctacgggc
      241 gcgattgcac ggacggcgac cagcattcgt tcgggcgcgc aatcaccggt ggcggggatg
      301 atccatgcgc tgacactgct gtgcatcctg gtctttgccg cgcccctggt cagctatgtg
      361 cccatggcgg cgctggcggc aattctgatg atcgtcgcct acaacatggg ggagtggcgc
      421 gagattccgc aactgctgaa gttgaccaag actgacatca gcatctggct ggtgacattt
      481 gccctgaccg tgttcgccga tcttacggtt gcggtggaag cggggatgat tctggcggcg
      541 ttgctgttca tcagccgcgt ggccgcaacc actactgttt cgcaggtaac cgaggaatat
      601 gtggaggacg gacgcgtcca catcctgcag gacaaggaca ttccctacta cgcgaccatc
      661 ttccgcatcc acgggccttt cctgttcggc gcaaccgaca agattgcggc agtcacggag
      721 aacctgcacc gcttgccccc ggttgtcatt ctgcgtctgc gaaacatgac cgcgctcgat
      781 gccagtggtc tgtttgcgat cgaagaagtg gcgaaacagc tgcacgccag caaacgaacg
      841 ttgatcctgt gcggcgcgcg ggagcagccc cgcaagctga tgcatcaggc ggagttcgaa
      901 gaggtggtcg gcacagataa catctgcgac aacgtgcagg aagcactgcg acgcgcagaa
      961 gaagtgtacg aaggactcga ggccaacagc gccaccttgg gccgccgtta gttggaaatc
     1021 actgttgcag aacggcctgt gtcagtcgta ctcagatttt ctgctccttc cagcgccctt
     1081 gcctgaacag aaacgcgctg gcggcagcga tgctgccctc ggcgatcaca atggcccaga
     1141 acaccccgtt cgactccatg cgcagaagaa tggccagcga ataggccagg ggaatctcca
     1201 gcagccagaa gccgaagaaa ttcacgatgg ttggagtcac tgtgtcgcct gccccgttgt
     1261 aggcctgcag catcaccatt ccataggcgt aaccgatgtt gccgtagctg acgatgcgca
     1321 ggcaggctgc tcccagggtc acgacgccag gatcgtgcgt gaacagacgg actgcaggtg
     1381 tggcatacaa aacgaaaaag actccgaccc ctcccaggaa catcatgttg taaagaccgg
     1441 tgcgccatac tgcggtctcc gcgcgctcgg gctcgccggc gcccaggttc tgccccacca
     1501 gcgttgcggc agcgttgctc aaaccccacg aaggcagaat cacaaacgtg acaatgcgca
     1561 tcgcgatggt gtatcccgcc agagccgaag cgccgaagat gctcacgatg cgcaccaagc
     1621 cgatccaact ggtgtgggcg atggcgaact ggagaatgcc ggtcagcgaa actcgtacca
     1681 ggcgccacag cacgtccact tgcagccgga tctgccggac aaggacgcgg atccgctccg
     1741 ttcccttgag taaccggtag aactggtaca tcactccaac accacgcccg ataaacgtgg
     1801 ccagcgccgc tccggtgacg cccaggcgcg ggaaaggacc caggccaaaa atcaggcaag
     1861 gatcgaggat cagattgatg atgttcgaca cccagagcag gcgcatggcg atagcggcat
     1921 cgcccgctcc gcgaaagatg gcattgttca ggaacaacaa caaaacaacc cagctcccgc
     1981 cgaggcagat ccgggtatag ccgctgccca cggagacgat ttccggactc gcgcccatcc
     2041 attgcaacag gcgcggagca agaatcagac agggcagacc aatcacgaag gacacaacga
     2101 gaccaattgc gatggcctgc accgcggcga cggctgcacc ttcgtgatct ttctcgccaa
     2161 tgcggcgagc caccatcgcc gtcgtcgaca ggctcagccc tagccccacc gagaagacaa
     2221 tgtagagcat ggactcagtg agtcccacgg cagctacggc gctggcgccg aggcggccaa
     2281 cccagaacac gtccacaacc gcgaaaagcg actcgagagc catctcgaga accatgggca
     2341 ctgccagcag aaagatcgcc cggttcaaac ttcccgcggt gtagtcctga tgagaaccgc
     2401 gaaccgcctc gaccagcgag gtccagagag aaagatgcgg cttggcggca gatgaaatcg
     2461 cttccataag ttccgggctt tccacggccg catccgcgct gtgcacggag gcggctgctt
     2521 ctgaactatc aagtgaggta gaccagaaaa cccgctgtga tgcgggttaa acggaacggc
     2581 gagttagcgc ggcatgaaca tgacgttctt cagagtggac tgtttcgaat cggaggtctc
     2641 gagaattcta cagcaggggc cgactctttg acaaagaaaa cagcaagcgg cgaggaatta
     2701 ggttctactc gccgcttgct ctggaggagg atcggtgatg ttttgttctg agacaacact
     2761 agggtaaggc caagggccaa agctggctag atcacaaagg tacaaatcgg gaacgcggac
     2821 tttccatttt gggggtagtc ccaatcggga atgttacttc ttgggcagca gaaacacgcg
     2881 agcggttgca gtctggttac tgaccatccc ccccagaatg accgcaccta ctggagttga
     2941 aagcatcccg cgggtgtctg agcgcggatc ataggtttct tcgtcgagcg tctcccagcg
     3001 gttgccgtgc acatcgaagt cgaaagtaac gggcgaaagt tccacgggct tcccttcgtg
     3061 gtccagtcct ttgaagctgt gaacggcggg ggaccctccg gaaaagatca ctttgctgcc
     3121 ccttgcgccg gccacaatgc cgaagtgtgc tggcccggga tgtggtggca gtttggccca
     3181 ctcgaccttg tcggggtcct tgtggtcgat tttgccgatc cagcactcgt cggattccac
     3241 gtagggagcg ccgaaagcgg tgttctt
//
LOCUS       DASPUL010000278         6353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_596283, whole genome shotgun sequence.
ACCESSION   DASPUL010000278 DASPUL010000000
VERSION     DASPUL010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596283"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5955)
                     /locus_tag="VEJ00_06840"
     CDS             complement(<1..5955)
                     /locus_tag="VEJ00_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXX00913.1"
                     /translation="MDDSGKADLIGRTLAHYRIQSAIGAGGMGEVYRATDTKLGRDVA
                     LKVLPPDMSRDPDRLARFQREARAVAALNHPNVVTLYSVEESDNVHFITMELIEGQPL
                     DRLISANGLPADRIIEIAGAVAEALAAAHEKGIVHRDLKPANVMVTNEGRVKVLDFGL
                     AKDVGAETANDATLTSAGRTQAGMVMGTPAYMSPEQVSGRPLDHRTDIFSLGVLLHEM
                     ATGRRPFEGTSSAELVSSILRDSPPSVADARPELPSDLARVIRRCLEKDPRHRMQTAR
                     DVSNEFRDMARTTKRSVTVSTSSTRATAAADSGKARADEGFWVAVLPFKYGGNDADLM
                     ALSEGLSEEVITGLSRFSYLKVIARSSTARYAGQSVDVRSASKELNARYVMEGTLRQA
                     GSRLRLAVQLVDTVSGAHLWAENYERVFSPEAVFELQDDLVPRIVSTVADINGVLPRS
                     LSEAVRQRAPEQLTPYEAVLRSFGYFDRVTAEELNAAMAGIKSAVEKAPTYADAWAML
                     ALLSVQRYAQGFGREADALTRGAEAARKAVEAAPANHLAWFSLSQVLFFQREFPAFRN
                     AAERALALNPMDSNSLAFHGEMLAYSGEWERGLSLAERARQLNPHHPAWYWHVNFNHA
                     YRQRDYRAALDIAGKMNQTGNWGAHLLTAAACGQVGDLGGAARALREVLKLRPSIASA
                     ISADADKWFDAEHAAHLLEGLRKAGLEIPAEGQTRSPAVPASSAIRDSGAARAALRED
                     EGFWIAVLPFKYKGADSGLEGLADSLTEEVITGLSRFSYLRVIARGSTAKYSRDSGDL
                     RAISKELGARYVMEGSLRQAGSTIRLATQLVDATTGATLWAETFERAFRPDEIFALQD
                     QLVPRIVSTVADQHGVLPRSISAAIRKKNDDQVTPYEAVFRVFSLHEKMTEQDHAACR
                     DLLERMVRDAPDAADCWAMLATLYADEDWFGFNLRPDALGRAAAAAQKAADLGPASVL
                     ASQALAQSLFMRHEWQAFRPVAERTIALNPMDGAIVAVMGLMLACSGDWERGCAVADS
                     AMRLHPNFPGWYWLATVFNAYRTGDYRAALDAALRIQMPGYFWDSVTRAAAYGQLGDL
                     RSAQKALRELLVAKPDFAKTARQELEKWFEPELAGHFLEGLTKAGLEDAAGSATPAAG
                     SSGAVRTQPISGPTRADEGFWVAVLPFKCVGGSADLAALAEGLTDDIVTGMSKFSYLR
                     VVARTSTARYAKQAVDVRTAAKELGARYVMEGSIRQAGAKLRIAVQLVDAVSGAGLWA
                     ETYDRAYSPETTLDLLDDVVPRIVATISDTQGILVHSMTDALRNHDPETLTPHEAVLR
                     SFGYYQRTNATEHLAARTALEHAVKQPPDRADCWATLAWLYREEYTHGFNLQPDPLGR
                     ALAASRRALDADPTNHLAHTALAPTLFFLHDVPGFRAAAERAIILNPLEGYSLAYLGM
                     LIAYSGDWQRGCALADRAMQLNPNHPGWYWFPRAFDAYRQGDYRGALGTALRVNLPGF
                     WRTQLILAVANGQLGERVAAGIAVQELLKIKPDFAITAREELKKWYQDELIIHLIEGL
                     RKAGLEIPDADSKPAPSGAQPARTASGASRANEGFWVAVLPFKYTGSNTDLKGLAEGL
                     SEEVITGLSRFSYLRVIARGSTAKYSSESGDIRSIGKELGARYVMEGGLRQSGSKLRL
                     AVQLVDATTGAHLWAETYERAFTPESVFEIQDDLVPRIVSTVADQHGVLPRSMSEVLR
                     SKSEGSLTPHEAALRGFSYFTRLTREDHATIRRILEKAVREAPDQADCWAMLALMYIV
                     EYSDGFNALPNPLDRALAAAQRAVDVGPTHALGHYALAFVYFFRKEKAPFRAAVEQTL
                     AINPMDGTLIGLLGLLLHHSGDEERGLQLVKSAMQLNPNYPGLLRFTPFSDAYCHGRY
                     AEALEAAVRINMPGFFYAHASRAAALGQLGQHEAARKAVEELLALRPDFASAARLEYS
                     KWYEPEKVELFIEGLRKAGLEIPDEDS"
     gene            complement(6049..6177)
                     /locus_tag="VEJ00_06845"
     CDS             complement(6049..6177)
                     /locus_tag="VEJ00_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00914.1"
                     /translation="MADLRLIAESVAQHEKVMAALKRHKVEAGMQSEGKSRRNSST"
BASE COUNT         1186 a         2009 c         1948 g         1210 t
ORIGIN      
        1 ggaatcttca tcggggattt cgagacccgc cttgcgcaag ccttcgataa agagttcgac
       61 cttctcgggc tcgtaccatt tggaatattc caggcgcgct gcggaggcaa aatccggacg
      121 gagggcgagc aattcctcca ctgctttccg agcggcttca tgttggccga gttgacccag
      181 ggcggcggcc cgagaggcat gggcgtagaa gaagccaggc atgttgatac gtaccgccgc
      241 ctcaagagcc tccgcatatc tgccgtggca ataggcatcg ctgaacgggg tgaagcgaag
      301 caggccggga tagttgggat tgagctgcat ggcagacttc accaactgca aacctcgctc
      361 ctcgtctccg gaatggtgca gaagcaaacc aagcaggccg atgagagttc cgtccatggg
      421 attgattgcg agtgtctgct caaccgcagc gcggaaggga gctttttcct tacggaagaa
      481 ataaacgaag gcgagcgcgt agtggcctag ggcgtgggtt ggccctacat cgacggcccg
      541 ctgagctgcg gccaaagcac gatccagtgg attgggcaag gcattgaatc cgtcggagta
      601 ctcgacgatg tacatcaggg ccagcattgc ccagcagtcc gcctggtccg gggcttcccg
      661 caccgctttc tccaggattc gtcggatggt cgcgtgatcc tcgcgggtga ggcgcgtgaa
      721 atagctgaag ccgcggagcg cagcctcgtg tggcgtcagg gaaccttcgc tcttgctgcg
      781 cagcacttcg ctcatgcttc gcggcagaac gccgtgctga tcggcaacgg ttgagacgat
      841 gcgtgggacc aggtcgtctt gaatttcgaa gacggattcg ggagtgaacg cccgctcata
      901 ggtctcggcc cagagatgtg cgcccgtcgt agcgtccaca agctgaaccg ccagacgcag
      961 cttgctgccc gactggcgca ggccgccttc catcacgtag cgcgcaccga gttccttgcc
     1021 gatggagcgg atgtcccccg actcgctgga atatttcgcc gtcgatccgc gcgcgatcac
     1081 gcgcagatac gagaatcgcg acagtccagt gatcacttcc tccgatagtc cctcggcgag
     1141 tcctttcaga tcggtgttgc tgccggtgta cttgaagggc agcacggcga cccagaagcc
     1201 ttcgttggct ctagatgctc ccgacgccgt tcgcgccggt tgcgcgcccg agggcgccgg
     1261 tttggaatca gcatcaggaa tctccagccc cgccttgcgc aggccttcga tcaagtggat
     1321 gatcagttcg tcctgatacc actttttcag ttcctcgcgc gcggtaattg cgaaatcggg
     1381 tttgatcttc agcagttcct gaaccgcgat gcctgccgct acccgctcgc ccaactgccc
     1441 gttcgcgact gccaggataa gttgtgtgcg ccagaagcct ggcaggttca ctctgagtgc
     1501 ggtccccaaa gccccccggt agtcgccttg gcggtaagcg tcaaaagccc ggggaaacca
     1561 ataccagcct ggatggttgg gattaagttg catcgcgcgg tctgccagtg cacaaccccg
     1621 ttgccagtcg ccgctatagg caatcagcat gccgaggtaa gcgagggagt atccctccag
     1681 cgggttcagg atgatcgcgc gctcggctgc ggctcggaaa cccggtacat cgtgaaggaa
     1741 aaacaacgtc ggggcgagag cagtgtgcgc gagatggttt gttgggtctg catccagggc
     1801 tcgccgcgac gcagccagcg cccgcccgag gggatctggc tgcagattga aaccatgtgt
     1861 gtattcctcg cgatacagcc atgccagagt cgcccagcag tccgcgcggt cgggaggctg
     1921 tttgacggca tgttccaaag ctgttcttgc ggccaagtgc tcggttgcgt tagtacgttg
     1981 ataatatcca aagctgcgca gcaccgcctc gtgtggcgtg agcgtttcgg ggtcgtgatt
     2041 gcgcagtgca tcagtcatgc tgtgcacgag aataccctgc gtatcggaaa tcgtggcaac
     2101 gatgcgcggg acgacatcgt cgagaagatc aagggtcgtc tccggactgt aggcgcggtc
     2161 ataggtttca gcccacaatc ccgcaccgct gaccgcatcc accaactgta ccgcgatgcg
     2221 aagcttcgct cccgcctgcc ggatgctgcc ttccatcacg tagcgcgccc caagctcctt
     2281 ggcagcggtg cggacgtcca ccgcttgttt cgcatagcgc gccgtggagg tgcgcgcgac
     2341 aacgcggaga taggagaatt tcgacatgcc ggtgacaatg tcgtcggtta atccttcagc
     2401 gagagcagca aggtcggcgc tgccaccgac acacttgaac ggcagcaccg caacccagaa
     2461 gccttcgtcg gcccgcgttg ggcctgatat cggctgagtg cgaaccgcac cggatgagcc
     2521 cgccgccggc gtggcgctgc ctgccgcgtc ttcgagtccc gctttcgtca ggccttcgag
     2581 aaaatgccca gcgagctccg gctcaaacca cttctcaagc tcctggcgag ccgtcttggc
     2641 aaagtcaggc ttggccacca gcaattccct gagtgccttt tgtgcacttc tgaggtcgcc
     2701 aagttggccg tacgctgcgg cgcgggtcac cgaatcccag aaatatccgg gcatctggat
     2761 gcgcagcgcg gcgtcgaggg cggcccggta gtcgcccgtc cggtaggcat tgaagaccgt
     2821 tgccagccag taccagccgg gaaaattcgg gtgcagccgc atggcggaat cggccacggc
     2881 gcaaccacgc tcccaatcgc cagaacacgc cagcatgagt cccatgaccg cgacgattgc
     2941 gccatccatg ggattaagcg cgatggtgcg ctcggccacg ggccggaacg cttgccattc
     3001 gtggcgcata aacaaactct gcgcgagggc ctggctggcc agaacgctgg ctgggccgag
     3061 atcggctgcc ttctgtgcgg ccgcggccgc acggcccagg gcatcggggc gaagattgaa
     3121 cccgaaccaa tcttcgtcgg catacaacgt ggccagcatg gcccagcaat cggctgcgtc
     3181 tggcgcatct cgcaccatgc gctccagcag gtcccggcac gcagcgtggt cctgctccgt
     3241 catcttttcg tggagactga aaacccggaa caccgcttcg tagggagtga cttggtcgtc
     3301 gttcttcttt cgaatcgccg cgctgatgct ccgcggcagg accccatgtt ggtcggcgac
     3361 cgtcgagacg atgcgcggga ccagctgatc ctgcagggcg aaaatctcat ccgggcggaa
     3421 ggcgcgctcg aacgtctcgg cccataaggt ggctccggtg gttgcgtcca ccagctgtgt
     3481 cgccagacga atcgtggagc ccgcctggcg aaggctgccc tccatcacgt agcgcgcgcc
     3541 gagttccttg ctgatggcgc gaagatcgcc agagtccctc gaatatttcg cggtcgagcc
     3601 gcgcgcaatc acccgcagat aggagaatcg agacaatccg gtgatcacct cttcagtcag
     3661 gctgtcggcc agaccttcga ggcctgaatc cgcgcctttg tacttgaacg gcagtacggc
     3721 gatccagaaa ccctcatctt cccgaagcgc ggcccgcgct gcgccggagt cgcgtatcgc
     3781 ggaagaagcg gggacagcgg gcgaccgtgt ttggccctcc gccggaatct ccagccccgc
     3841 tttccgcagg ccctccaaaa gatgggccgc gtgctccgcg tcgaaccact tatcggcgtc
     3901 tgcgcttatg gcggatgcga tggacgggcg gagcttcaac acttcgcgca aggctcgggc
     3961 agcgccgcct aggtcgccca cttgtccgca ggctgccgcc gtcaacagat gtgcgcccca
     4021 gttgccggtc tggttcatct tgcccgcgat atcaagcgcg gctcggtaat ctcgctgccg
     4081 gtacgcatgg ttgaaattaa cgtgccagta ccaggcggga tggtgggggt tgagctgccg
     4141 cgcgcgttcc gccaacgaaa ggccccgttc ccactcgccc gaataagcca gcatctcgcc
     4201 gtggaaggcg agagaattgc tgtccatcgg gttcagcgca agcgctcttt ccgccgcatt
     4261 gcggaaggcc gggaattctc tttgaaagaa caacacctga gaaaggctga accacgcgag
     4321 atggttggca ggcgctgcct ccaccgcttt ccgtgcagcc tctgcgcccc gggtcagcgc
     4381 atccgcctcc cggccgaatc cctgcgcgta acgctgtacg gacaacagcg ccaacatggc
     4441 ccacgcgtcc gcataggtcg gagctttctc cactgcagac ttgatcccgg ccattgctgc
     4501 attgagttct tctgcggtca cgcgatcgaa gtatccgaag ctgcgcagta cggcctcgta
     4561 gggggttaac tgctcagggg cacgctggcg gacggcctcg ctcagactgc gaggcagcac
     4621 tccgttgatg tcggcgacgg tcgagacgat gcggggaacc aggtcgtcct gcagctcaaa
     4681 cactgcttct ggactaaaga cccgctcata gttctccgcc caaagatgcg ctccggatac
     4741 tgtgtccacc aactggaccg ccagccgtag cctgctgccg gcctgacgaa gcgttccctc
     4801 catcacgtag cgcgcattca attccttgct ggcactgcgg acatccaccg actgaccggc
     4861 atagcgcgcc gtcgagctgc gggcaatcac tttgaggtag gaaaaccgtg agagcccggt
     4921 gataacctcc tccgacaacc cttcggaaag tgccatcaga tccgcgtcgt tgccgccgta
     4981 cttgaacggc aacacggcga cccagaagcc ttcgtccgcg cgggccttcc cggagtcggc
     5041 cgcagccgta gcccgggtag acgatgtaga aacggtaacg gagcgcttgg tggtccgtgc
     5101 catgtcacgg aattcgttgc tgacgtcgcg cgccgtctgc atgcgatggc gcggatcttt
     5161 ctccagacag cgccggatca cgcgggccag gtcggaaggc agttccgggc gtgcgtcggc
     5221 gacggacggg gggctgtccc gcagaatcga agacactagc tccgcagaag acgttccttc
     5281 gaacggacgc cggccggtag ccatctcgtg cagcagcacg ccaagagaaa agatgtcggt
     5341 acgatggtcc agcgggcgac ccgagacctg ttccggcgac atgtaggccg gagttcccat
     5401 caccatgccg gcctgggtcc ggcccgccga ggtcagggtc gcgtcgtttg cagtctccgc
     5461 gcctacgtcc tttgccaacc cgaaatccag aactttcacc cggccttcat tcgtcaccat
     5521 cacattggcg ggcttcaggt cgcggtgcac gatgcctttc tcatgcgccg cagccagcgc
     5581 ctcggcaacc gcgccggcga tttcgatgat ccggtccgcc ggcaagccat tcgcagaaat
     5641 cagccgatcc agtggctgcc cctcgatcaa ctccatggtg atgaaatgga cgttatccga
     5701 ttcctcgacc gagtagagcg tcactacgtt gggatgattg agtgcagcga cagcccgagc
     5761 ctcccgctgg aagcgggcca gccggtcggg atcgcgggac atgtcgggcg gtagaacttt
     5821 aagggcgacg tcgcgcccga gcttggtgtc ggtcgcgcgg tacacctctc ccatgccgcc
     5881 cgcgccaatt gcgctctgga tgcggtaatg agcgagcgtg cggccgatca gatcagcctt
     5941 acccgagtcg tccacctgtc gactctccct gcctggacaa aggcagcgcc gattctacat
     6001 catgaggccc cgggaaattc ctccctcagg tgacgtggag gcctcacgct aagtgctgct
     6061 gtttcggcgg ctcttgcctt cgctctgcat cccggcttcg actttatgcc gcttcagcgc
     6121 ggccatgacc ttctcgtgct gcgccaccga ttccgctatt agtcgcagat cggccatcta
     6181 cgccgcctca tacgcctgtg tcgagtgccg gcgctcgcgc gatcccgcaa attcgcgaat
     6241 gattcccctc ccgacaaaga tcgaagcgtg tgattcgcac ctgcatccaa cgctaagggg
     6301 tgcagggcag tgggctactg gtaagctgta acgtaggatg tcatcctgaa ggc
//
LOCUS       DASPUL010000279         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1962850, whole genome shotgun sequence.
ACCESSION   DASPUL010000279 DASPUL010000000
VERSION     DASPUL010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962850"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..317)
                     /locus_tag="VEJ00_06850"
     CDS             complement(108..317)
                     /locus_tag="VEJ00_06850"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXX00915.1"
                     /translation="MTIRGPQVVDFLGHLLRHLPGKLLEVCARLPAHRRRMVSFSTAP
                     GGRLAIEWLPRYAHELNPIEYAWDH"
     gene            1076..1606
                     /locus_tag="VEJ00_06855"
     CDS             1076..1606
                     /locus_tag="VEJ00_06855"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HXX00916.1"
                     /translation="MIGLNRGQVCSAPLKAKKLSQPRGVRRRATDGQAMAEFLMVAPL
                     FFFLIFAVFDYAHLFLVQMDIENAVQEAGRFASTGNHLPDPKNPGQYLSRVNSIIATL
                     QQAAYGQQITNIQISSLLGGNGSAGGPGDTVTVSVTVNLKLMTPVIARAFPNGTYTFT
                     SSTSFKNEPFPSTSTT"
     gene            1621..2157
                     /locus_tag="VEJ00_06860"
     CDS             1621..2157
                     /locus_tag="VEJ00_06860"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HXX00917.1"
                     /translation="MKPSACSGSVCAKIATTEHGQAAMEFGLLVTLMLVLFFMVIDFG
                     RAIHDVQVIAGLSRQGSNLASRGTSLSGSATAVLQGDSSLNLSNNGEVIITSVSNINK
                     VYTITGQVSQGQITQTSRVGNGVGNQATVPSSAALLLAPNQTIYVTEVFYSYRPITPL
                     KNLLKLALPSTLYQAAYF"
     gene            2176..3390
                     /locus_tag="VEJ00_06865"
     CDS             2176..3390
                     /locus_tag="VEJ00_06865"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HXX00918.1"
                     /translation="MRLTTNRRSARAQIFLLFALSLPVLILFVGLAVDLGFAFVTRAN
                     LSKAVDAAALAAMRNINQGQAQATAIAQSAFNTNYGSGIGRSLGPPSLNVVITTSANA
                     TSNAVVNVSATTSINTFFLGLVPGLKTLQIASSAQATRPNLIMSLVLDRSGSMNLNGG
                     AQALPPAVDNFLTYFDNNTDQIADVSFSSVASVDVSIRTNFLNPITNAVNKMAFGGAT
                     YSQGGLLDGQAQINSVTVAPGTNVVKVAVFFTDGWANTVEDNLNCPPSTLLNFGGCAP
                     PEAAVGWCSGISFLDPVTGNSRSCGATSFPSQMTGGSLALTQTNIANEAMYRAVQVAN
                     SMRSQGMVIYAIGLGDKISQAFLQQVANDPASSTFNPNTPVGEAVFAPTAADLQGVFQ
                     TIASEILLRLSR"
BASE COUNT          871 a         1200 c         1222 g          879 t
ORIGIN      
        1 acgagcggat tagcggcggc cgccggcaga tgcgccgcag ggcgcggcgg gcctgatggc
       61 tgggttgggc gaaatcgcgc gggcagaagt tgggcagttc gggttgttta gtgatcccag
      121 gcgtactcga tcgggttgag ctcatgggcg tagcgcggca gccactcgat ggcgagccga
      181 ccgcccggcg cggtgctgaa gctgaccatc cgcctccggt gcgccggcag tcgggcgcac
      241 acctccaaca gcttgcccgg cagatgacgc aacagatggc cgagaaagtc caccacctgc
      301 ggtccgcgga tggtcatagg gcagagccgg aagtagaagt tccatcaggt gatgccggcc
      361 gccccgacag caccttccaa ttgaaatgat actgcacacc ggagtgagcc cgtgcggggc
      421 tcaggtgcgc acccggtgcg gccgttcgct cagtccgctt tggtcatgaa gatgatcgtg
      481 cgccgctcgt agaaggcttt tcctctaaga aactccatcg ttcgagacag aaactccatt
      541 cgggagcggc tcgatgacgt tgcgcttctt atgggcttgg cggcgccgga gtggtgcgcg
      601 ctcgccaaag acctcgagcg ctgccctggc gcaaggcctt ggcgccgtcg atcaccacca
      661 agccggccgc gattggggtt gagcccgcgc gcgatccgtt cggcgagcag ccgcaaagcc
      721 gagccctaaa cggcctctgg cctgtggcca acctgctcca atgcatcagt agctcaaaga
      781 tgttcggtag ctgtggaccg ggcgcgtgct atctcgaaga tgggttgatc cggtatgcgc
      841 aataaacaag catttagagc tgtttagtca ttgatacgct aataattaga tgatgaataa
      901 agatatttag acttattttt cccagaaatt ctctcagtga tagattttta cagccagacc
      961 tagaaaatag gttaatctaa ctggtgatag gtttaatttg tctgtgctag gttctgttat
     1021 ctttcgatct ttgagggcac gacggatgac tgtaaaaaac gcctccttag gtccgatgat
     1081 cggtctcaat cggggccagg tctgctcggc gcccctcaag gccaagaaac tgtcccagcc
     1141 gcgtggagtc cgtcggcggg cgacagacgg ccaggccatg gccgagtttc tcatggtcgc
     1201 gccgctgttc ttctttctga tctttgcggt gttcgactac gcgcatctgt tcctcgtaca
     1261 aatggacatt gagaatgcgg tccaggaggc aggccgattc gcctcgacgg ggaatcatct
     1321 tccggatcca aagaaccctg gacagtatct gtcgcgcgtc aactcgatca tcgcgacgct
     1381 tcagcaggcc gcctacggtc aacagataac caacattcag atctccagcc tgctcggcgg
     1441 caatggcagc gcgggcggtc cgggagacac ggtcacggtt tcggttactg tcaacctcaa
     1501 gctgatgacc ccggtcatcg cgcgtgcgtt tcccaacggg acgtacacct tcacctcgag
     1561 caccagcttc aagaatgaac cttttccttc caccagtacg acttagccag gggtgacggc
     1621 atgaaacctt cggcatgctc cggttcagtg tgcgcaaaga tcgcgacgac cgaacatggt
     1681 caggcggcga tggagtttgg gctcctcgtg accctgatgc tcgtcctgtt tttcatggtg
     1741 atcgacttcg gccgggctat tcacgatgtg caagtgatcg cgggcttatc cagacaaggt
     1801 tccaacctcg catcacgcgg tacttcgttg tcgggctcgg ccacagccgt gctccagggc
     1861 gattcgtcgc tcaatctgag caacaacgga gaagtgatca ttacctcggt cagcaatatc
     1921 aacaaggtct ataccatcac cggccaggtc tcgcaggggc aaatcacgca aaccagccgg
     1981 gtggggaacg gagtagggaa ccaggcgacg gtgccgtcgt cggccgccct tctgctggcg
     2041 cccaaccaga cgatctacgt aaccgaggtg ttctattcgt accggccgat aactccgctt
     2101 aagaatttgc tcaagctcgc tttgccatcg acgttgtatc aggcggcgta tttctaagac
     2161 aggcgtaggt gtaacgtgcg actaactacc aatcgacgat cggcgcgggc gcaaatcttc
     2221 ctgctgtttg cgcttagcct gccggtgctc attctgttcg tcggtcttgc ggttgacctc
     2281 gggttcgcct tcgtcacgcg ggcgaatctc tccaaggcgg tggacgccgc cgcactggcg
     2341 gccatgcgca acatcaacca ggggcaggcc caggcgacag cgatcgcaca gagcgcgttc
     2401 aataccaact acggttcggg tattgggcgc agcctcggcc cgccgtcgct caacgtagtc
     2461 atcaccacaa gcgccaacgc caccagcaac gcggtggtaa acgtcagcgc tacgacctcg
     2521 atcaatacct tcttccttgg cctggtgccg gggctgaaga ccctgcagat cgcttcgagc
     2581 gctcaggcga cccgtcccaa tctgatcatg tcgctggtgc tggacaggtc gggctcgatg
     2641 aaccttaatg gcggagcgca ggcgcttccg cccgcggtcg ataatttcct gacgtacttc
     2701 gacaataaca cggatcaaat tgcagacgtc agtttctcct cggtcgcttc cgtcgatgtg
     2761 tccatacgca caaacttcct caatccgatc accaatgcgg tcaacaagat ggcattcggt
     2821 ggcgcaacct attcacaggg cggattgctg gatggacaag cgcaaatcaa cagtgtgacg
     2881 gtcgcgccgg gcactaacgt ggtcaaagtg gcagtctttt ttaccgacgg ttgggccaat
     2941 accgtcgagg ataacctgaa ttgccccccg tccacgcttc tgaatttcgg cggatgcgct
     3001 ccacctgagg ccgccgtggg ctggtgcagc ggcatctcgt tcctggatcc ggttaccggc
     3061 aactcgcgga gttgcggagc gacctctttt ccttcgcaga tgaccggagg gtctctagcg
     3121 ctcacccaga cgaatattgc caatgaggcc atgtatcgcg cagttcaggt agcgaacagc
     3181 atgcgcagtc aggggatggt gatttacgcg atcgggctag gcgacaagat cagccaggcg
     3241 tttcttcaac aggtcgccaa tgatccggcc agctccacct tcaatcccaa cactccggtc
     3301 ggagaggcgg tcttcgcgcc aaccgcagcc gacctgcagg gcgtctttca gactatcgcc
     3361 tcggagatcc tgctccgtct gtcgcgatag cggttgacgg cacgaagcaa tgccgattcc
     3421 gagatgggat catagtggcg tatcgatgtg tcgctgaaag agtgcatgac gtcagagagt
     3481 ccaggtcgcc gatctctact gagaaatcca gttggagaat aaaccagctc aatcgtcccg
     3541 acggattgag cacggaaaac gattggtcct ttcccagagg gctccgatct gaaagatgct
     3601 aaaagtttgc ttgatctgct gaacacatag gtacgaacgt ccggcgtttt gtgatcaacg
     3661 aatgctctcg gtttttcgag gcgaaggagt tagtcatgga caagtcaact gccatcaggt
     3721 tagtgcctgg aagcgccgtg ctttttcgcg ggcaccgtca ttcagttatg agcatcaaga
     3781 gcggacagca aggcgaggcg ccgttctttc gtctgcgtgc tctcgacgac ggcacggtaa
     3841 cgggcttgat cagccacaga ctgattgaac gagcaacaga agagtccggc agggaacagt
     3901 ccgcgcaaag ctagcgcgat tcgtctgacg atgcttgcca ggcatggacg gggtcgctgc
     3961 acagtggatc ggtgatacgg acgtcgatgc tgctgtcgct cagacacgcg agcccggtcg
     4021 cagggttgag acaatcgctg tggcggagct caaccatctc gtcggcgtag aagctcatgc
     4081 ggccgttcag atagagctta acttgatacc gccaccgcct ctgggtcgaa ctcgagatgg
     4141 ccgcacacca ggttgatgcc tatgagaaca cc
//
LOCUS       DASPUL010000280         5036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_875589, whole genome shotgun sequence.
ACCESSION   DASPUL010000280 DASPUL010000000
VERSION     DASPUL010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875589"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VEJ00_06870"
     CDS             complement(<1..301)
                     /locus_tag="VEJ00_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HXX00919.1"
                     /translation="MRPKPLILIILDGWGYRAETKANAIALARKPTYDRLLRDYPNTL
                     IHTSGPYVGLPEGQMGNSEVGHLNIGAGRIVHMDITRIDLMVQNGELFQHPVLLDA"
     gene            complement(396..896)
                     /locus_tag="VEJ00_06875"
     CDS             complement(396..896)
                     /locus_tag="VEJ00_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX00920.1"
                     /translation="MINELLLNEFDEEIKKTRALLERVPMKADFAPHPKSMPLGKLAP
                     HVAELAGFGYTVLTTPELDFSAGPPRTRPPFESPEQLVKAFDEGAAKVRAALKDIPDA
                     AWNEPWKLSLQGKPIFSGTRFLAYRQMFLNHLVHHRAQLGVYLRLNDTPLPATYGPSA
                     DDRMGF"
     gene            complement(1165..1377)
                     /locus_tag="VEJ00_06880"
     CDS             complement(1165..1377)
                     /locus_tag="VEJ00_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00921.1"
                     /translation="MPDSEIIFSVQESAEGGYEARALGHSIFTQADTMEELKSNVREA
                     VHCHFEEGDAPSVIRLHMVKDEVIPI"
     gene            complement(1418..2704)
                     /gene="eno"
                     /locus_tag="VEJ00_06885"
     CDS             complement(1418..2704)
                     /gene="eno"
                     /locus_tag="VEJ00_06885"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXX00922.1"
                     /translation="MSNIAAIHAREVLDSRGNPTVEAEVFLADGSMGRAIVPSGASTG
                     EHEAVELRDGDTNRFQGKGVLKAVENVNGEIAEALANWDAFDQRSLDRKMIELDGTEN
                     KGRLGANSILSVSMAAARASAQSLDIPLYRYLGGAGANTLPVPMMNILNGGAHADNNV
                     DFQEFMVMPVGAPSFSEALRWGVEVFHTLKGVLKKRGYNTAVGDEGGFAPSVKSNVEA
                     IEVVLEAITKAGYKPGEEIAIALDPAASEFYEDGKYVFKKSDKSTKSSDEMVRFWAKW
                     VNDYPIVSLEDGLSEDDWDGWANLTKELGGKIQLVGDDIFVTNIQFLQEGIDKGVANS
                     ILIKVNQIGTVSETLDAIDLARRNGYTSVISHRSGETEDTFIADLAVATGAGQIKTGS
                     ASRTDRIAKYNQLLRIEEELGGAARFLGLKALNYRG"
     gene            3070..3723
                     /locus_tag="VEJ00_06890"
     CDS             3070..3723
                     /locus_tag="VEJ00_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00923.1"
                     /translation="MTKKWILLPCLLAATLAAQTNAPVPAGTALMVRLDTTLATFSNR
                     AGDPFQGRVTQAVVVNGVTVIPAGATVEGRVTKVSEPRRISGKPTIGILPEAVILPTG
                     ERYYLDATLVDTNIRGTDVNDEGQFKGSGHDRRDEIETGGGTAGGMLIGGLAGGPVGV
                     LVGGIVGAGATTGHWLAKHRSATLPAGTQLTLELNRPLTMTAPPTTTTASAPSGGSK"
     gene            3832..4059
                     /locus_tag="VEJ00_06895"
     CDS             3832..4059
                     /locus_tag="VEJ00_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00924.1"
                     /translation="MRTTVDIPDPIYRALKKKAAHEGHSVKELVLRGVELELRAEPRK
                     RGRRRPPVIDSDKPGSLDLDNAKIYEIIPFP"
     gene            complement(4484..>5036)
                     /locus_tag="VEJ00_06900"
     CDS             complement(4484..>5036)
                     /locus_tag="VEJ00_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00925.1"
                     /translation="GAAVYTADHRAGAMHGKQFLLDSVYAGIDATHVYGRLDFKDKVP
                     GAELELVVNVESWAGGEARPRRALRVDAVVQTQKLREWKIESASNARLLASSAQPDDH
                     AKLALLRNFEFKLPLAWLLATPGAASQPRAAESRGPAAVAAASKLRLRFSLWQNRLPV
                     DSLPLEGWIELQVVSEGELLFGA"
BASE COUNT          965 a         1467 c         1551 g         1053 t
ORIGIN      
        1 tggcatcgag cagcactgga tgctggaaca gctcgccgtt ctggaccatc aggtcgatgc
       61 gggtgatgtc catatgcacg atgcggccgg cgccgatgtt gagatggccg acttcgctgt
      121 tgcccatctg gccttcgggc aagccgacgt aggggccgct ggtgtgaata agtgtattgg
      181 gatagtcgcg cagcaagcgg tcgtaggtgg gcttgcgcgc gagcgcgatg gcgttagctt
      241 tggtttcggc gcggtatccc cagccgtcga ggatgatgag gatgagaggt ttgggacgca
      301 aaagcagctc ctggctctta gctcctagct cttagctaat tcaaaatcgt tcgccaccga
      361 tttgcgcgga tgaacgcgga tcaaggactc aactcctaaa atcccatccg gtcgtctgcc
      421 gacgggccgt aggtcgccgg cagcggcgta tcgttcaggc ggaggtacac gccaagctgt
      481 gcgcgatgat gcaccaggtg gttcaggaac atctgccggt aggcgagaaa acgcgtcccg
      541 ctgaagatgg gcttgccctg aaggctgagc ttccagggct cgttccaggc tgcatcggga
      601 atgtctttga gcgcagcacg cacttttgcg gcaccttcgt cgaaggcctt caccagttgt
      661 tcgggcgatt cgaagggcgg gcgggtacgc ggcggaccgg cgctgaagtc gagttcaggg
      721 gtggtcagaa cggtatatcc aaaccctgct agctcagcca catgcggcgc gagtttgcct
      781 agcggcatgg acttgggatg gggcgcgaag tcggctttca tgggaacgcg ctcgagcaag
      841 gcgcgcgtct tcttgatttc ttcgtcgaat tcgttgagta ggagttcgtt gatcatgtgt
      901 cctcatttgc cgcggatggg ttatttctcg aacagatccc gtgctagttt tgcgcgatct
      961 acgccaaggt agtctgccac atcggagagg atgccattta gggtgccaat ccgcaggttg
     1021 tcatgatcgg gaatcgtgat aaggtgcgct tgacctcgca agttcgactg cagccgaatg
     1081 tggctgccga cctgccgggt agactcgtag ccatagcgct tgagtagccg aatagttcac
     1141 gaccggaaac atcgcgcggc agcctcaaat cggaatgacc tcatccttca ccatgtgtag
     1201 gcggatcaca ctgggcgcgt caccttcctc aaaatggcag tgaaccgctt cccgcacgtt
     1261 cgatttcaat tcctccatcg tgtcggcttg ggtaaaaatc gagtgaccga gggcacgggc
     1321 ttcatagcca ccctccgcgg attcctggac cgaaaagatg atctcactat ctggcatgtc
     1381 gagaattata ctcggcaaca acttcgcagc ttgaccgtca ccctctatag ttcaacgcct
     1441 tcaagcctaa aaaccttgcc gcgccgccca actcctcttc aatccgcagc agctgattgt
     1501 acttggcaat ccggtcggtg cgggaagccg atccggtctt gatctggcca gcgcccgtcg
     1561 ccacggctaa gtcagcgata aacgtgtctt cggtttcgcc ggagcggtgc gagattaccg
     1621 acgtgtagcc gttgcggcgg gcgagatcga tggcgtcgag cgtctcgctg accgtgccaa
     1681 tctggttgac cttgatcaga atcgagttgg ccacgccctt gtcgatgccc tcctgcagga
     1741 actgaatgtt ggtgacaaag atgtcgtcgc ccaccagctg aatcttgccg ccgagttctt
     1801 tggtgaggtt cgcccagccg tcccagtcgt cttcggaaag tccgtcttcg agagagacaa
     1861 tgggatagtc gttcacccac ttcgcccaga agcgcaccat ttcgtcggag ctcttggtgg
     1921 atttgtcgga cttcttgaag acgtacttgc cgtcttcata gaactcgctg gcggcggggt
     1981 cgagtgcgat agcgatttct tctccgggct tgtaaccggc cttggtgatg gcttcgagaa
     2041 cgacttcgat cgcctcgacg ttcgacttca cagagggcgc gaatccgccc tcgtcgccga
     2101 cggcggtgtt gtagccgcgc ttcttcagca cgccttttag cgtatggaag acttcgacgc
     2161 cccagcgcag cgcttcggaa aatgacggcg cgcccacggg catgaccatg aactcctgaa
     2221 agtcgacatt gttgtcggcg tgggcgccgc cgttcaggat gttcatcatg ggcacgggca
     2281 aggtgttggc gccggcgccg ccgaggtaac ggtagagcgg aatgtcgagg ctctgtgctg
     2341 aagcgcgcgc ggctgccatc gataccgaca gaatggagtt tgcgccgagc cggcccttat
     2401 tctcggtgcc gtcgagttcg atcatcttgc ggtctaggct gcgctggtcg aatgcgtccc
     2461 agttggccag ggcctcggca atctcgccgt tgacgttttc gaccgctttg agcacgcctt
     2521 tgccctgaaa acggttggtg tcgccgtcgc ggagctcgac cgcctcgtgc tcgccggtgg
     2581 acgcgccgct agggacgatc gcccgcccca tcgatccatc ggctaaaaag acctccgctt
     2641 cgacggtggg gttgccgcgt gaatccaata cttctcgtgc atgaatggct gcaatatttg
     2701 acatagctta agaaggctcc ctgtgcgcaa tgatttggag caagtacgct tgccggtgtg
     2761 ttgtttcgaa ggctttgagg ccctcagggg ctaaagcgct ggtcccgagc ggatcaggac
     2821 ggcacagcta aaagccgtgc ccttcccaag accgactcgg actctgaaac cgacgattat
     2881 atcggatggg cgtgaacacg atgtgatggc gggcacagct cgaaagggcc gttttccctg
     2941 ttcgggaccg tccggtcggc cagccgggca ggctcttatc tccgctggtt cccctgtcct
     3001 gcctgcgtat tgtcggggat tccgggctgg tctagagtca aaataacttt cgtagtgtta
     3061 ggagctggta tgacgaagaa gtggatattg ttgccctgcc tgctggccgc aaccttggcg
     3121 gcccagacca atgcgcccgt gccggccgga acggccctga tggtgcgcct cgatacgacc
     3181 ctcgccacct tcagcaaccg ggctggagat cccttccagg gacgcgtcac gcaggccgtc
     3241 gtggtgaacg gggtaacggt gattcctgcc ggggcgacgg tcgaaggccg ggtcacgaaa
     3301 gtgagcgagc cgcggcgcat ctcgggcaag ccgacgatcg gaatcctgcc tgaggccgtg
     3361 attctcccca ccggcgagcg ctactacctg gacgcgacgc tggtcgatac aaacatccgc
     3421 ggaacggatg tgaacgacga aggccagttt aagggctcag gccatgatcg ccgcgacgag
     3481 atcgagaccg gcggcggcac tgccggcgga atgctcatcg gcggattggc cggagggccg
     3541 gtaggcgtgc tggtcggcgg aattgtgggt gcgggcgcga ccaccggaca ttggctggcg
     3601 aagcatcgct cggcgacctt gccagccggg acgcagctca cgctggaact gaatcgtccg
     3661 ctgacgatga cagcgccgcc aactactaca acggcttcgg ccccgagcgg cggcagcaag
     3721 taggattgcc gcaatcttcg gatcgagagc gccgggcccc gcgggtccgg cgctcttgtc
     3781 ttttccgggg agcatttgcg cattagccat aaatatggca gaatggacgc tgtgagaacc
     3841 acggtcgaca ttccggatcc gatctaccgt gccttgaaaa agaaggccgc gcacgagggt
     3901 cattcggtga aggagttggt tctgcgcggc gtcgaactgg aattgcgggc cgagcccagg
     3961 aaacgtgggc gcaggcgtcc tccggtgatc gattccgaca agcccggcag cctggatctg
     4021 gacaatgcca agatctacga gatcattcct tttccctgac gttaacgttt ggattgccct
     4081 cacctaccgc gcccacaaac accacatcgc cgccagaacg tggcttgatt ctcttgcgga
     4141 cgaatcggaa ctttgttttt gccggctgac ccagctcggt ttccttcgct tgctgactac
     4201 cgatgcagtg atgggaagcc aagtgctctc acaaacccaa gcgtgggaaa cctatgacga
     4261 ctggctacaa cgcggacaag ctatctacat ggatgagccg ccgaccgtag aacaggtttt
     4321 ccgatcgttc tcccagtcag ggcagccagc tccgaaggat tgggcagatt cctacgtcag
     4381 cgcctttgca caagcttcag gcatacaact cgtgacgttt gatcgcgcac tccacagaag
     4441 gacagcaaaa tcgatcctgc taggcgagta gttgggcccg acattacgct ccgaacagca
     4501 actccccttc gctgaccacc tgcagctcga tccaaccttc aagcggcaag gaatctaccg
     4561 gcaagcggtt ctgccacaag ctaaagcgga ggcggagttt gctggcggca gcgacggcgg
     4621 caggtccacg gctttctgcc gctcgcggct gagaagccgc acccggagtt gccagcagcc
     4681 acgccagcgg cagcttgaat tcgaagttgc gcagcagggc cagcttcgcg tggtcgtccg
     4741 gttgagccga tgatgccagt agccgagcgt tggaggcgct ctcgatcttc cattccctga
     4801 gcttctgcgt ttgcaccacc gcatcaacgc gcagtgcccg ccggggacgg gcttcgccac
     4861 cagcccatga ttcaacattg acgacgagtt ccagttccgc tccggggact ttgtctttga
     4921 agtccaggcg gccgtacacg tgcgtggcat ctatgccggc gtaaaccgaa tccagcagga
     4981 attgcttgcc gtgcatggcg ccggcgcggt ggtccgccgt gtaaacggcc gcgccc
//
LOCUS       DASPUL010000281         4360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1006498, whole genome shotgun sequence.
ACCESSION   DASPUL010000281 DASPUL010000000
VERSION     DASPUL010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006498"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1786
                     /locus_tag="VEJ00_06905"
     CDS             <1..1786
                     /locus_tag="VEJ00_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HXX00926.1"
                     /translation="EIGLQYNDGYSETLFSFANNINTVDGGTHLSGFRTALTRTINYA
                     GQQLGLFKDVKENLTGDDVREGLVAVVSVKLSQPQFEGQTKGKLNSDIAGVVTAFVNE
                     RLGAYFEQNSTVAKKIINKAVDAARAREAARKARDLTRRKGALDSGGLPGKLADCSER
                     DPNRCELFLVEGESAGGTAKQGRDRRFQAILPLKGKILNVEKARYDKMLAHEEIRAMI
                     TALGTGIAKDDFDPTKVRYGKIILMTDADVDGSHIRTLLLTFFFRHMQELIKRGNVFI
                     AQPPLFSLKKGKSQQYIKDEREFVKVMVKRAAEGLSVRYGEGAAKIEGKDLARFMTVL
                     DEYLGFFDKLDKRVRNERVTELIPKLDLAKRADFEGEKKAAPKKIERLEREIKKLQKD
                     EGFKSVEARFDEEHNLWEVSFVNKHGAEHVINWELASSAECRQMISKYKQIEAYMEPP
                     FVVETLVKAVAAGNNGEGADGEAETGEGESLGKEKAEKKTAKPAAKRKAEIEVVEKTN
                     VRDLRDYVINEGRKDFSIQRYKGLGEMTAPQLWETTMDPERRTLLSVKLEDIAECETI
                     FTTLMGEDVESRRKFIEENALDVKNLDI"
     gene            1926..3278
                     /locus_tag="VEJ00_06910"
     CDS             1926..3278
                     /locus_tag="VEJ00_06910"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HXX00927.1"
                     /translation="MQGFRSFVQLFLVLIAATLSLSPDDSSAAPTPPMGWNSWDSYGT
                     TVREEQVKANADWMAEHLAKYGWQYIVVDIQWYEPNAQGHEYKPGAPLTMDEYGRLIP
                     AVNRFPSSANGAGFKPLADYVHGKGLKFGIHIMRGIPRQAVEKSLPIKGTSFHAADIA
                     DRNNPCRWNSDMWGVDTTKSGGQAYYDSIAELYASWGVDFIKADDMGSHRFQPEEIKA
                     LSLALRKTGRSIVLSISPGPASLSDAEFFEKYAHMWRISDDFWDDWKLLRRQFDYMRA
                     WAPFVGKNDTWPDADMLPMGKLRVTATEGGGTPTKFTPDEQQTMMTLWSIFRSPLILG
                     GDLPSNDDATTALITNPEVLDVDQHSSGRHEVLVKGNLRVWVADGAKPGERYAAVFNL
                     GESAGPVSLDWREVGIQSPVVQVRDLWAHKMLGEVAGIHASLRPHASLLYRVMVSESH
                     "
     gene            complement(3417..>4360)
                     /locus_tag="VEJ00_06915"
     CDS             complement(3417..>4360)
                     /locus_tag="VEJ00_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00928.1"
                     /translation="QRIVARLPGALILLDIAKDGRVLLVRADWRRELLGLFTSDPKQH
                     ELSWLDYTYPADLSADGRTLLFDEEGGGGALDYSKSSGLTYAVYIRKTDGTPAVLLGE
                     GGAVALSPDGKWAISQTQDSPSQFKILPTGAGEPRELTKDNINHSWARWFPDGKRVLF
                     SANEPGKGVRFYVYDMASGKSQAISPEGVNGTAFSISPDSQQIAAIGPDQKGYLYPVA
                     GGEPRPIAGFNQGEQPVTWAGDGRSLYVYQPGELPARVYHVDLQTGQRTLWKELMPSD
                     PAGVENIGPILMTPDAKTCVFGYHRMLADLYLVEGLK"
BASE COUNT          942 a         1135 c         1468 g          815 t
ORIGIN      
        1 cgagatcggg ttgcagtaca acgatgggta ctccgagacg ctgttcagct tcgccaacaa
       61 catcaatacg gtcgatggcg gcacgcatct gtcgggcttc cgcacagcgc tgacccgcac
      121 catcaactac gccgggcagc aactgggctt gttcaaggac gtcaaagaga acctaaccgg
      181 cgacgacgtg cgcgaagggc tggtggccgt ggtcagcgtg aagctgtcgc agccgcagtt
      241 tgaagggcag actaagggaa agctgaattc cgatatcgcc ggtgttgtta ctgcgtttgt
      301 aaacgagcgg ctgggggcgt atttcgagca gaattcgacg gtcgcgaaga agatcatcaa
      361 caaggcggtt gacgcggccc gggcccggga agcggcgcgc aaagcacgcg atcttacacg
      421 gcgcaaaggg gcgctcgatt ccggagggct gccgggcaag ctggcagatt gttccgagcg
      481 cgatccgaac cggtgcgagc tgttcctggt cgagggcgag tcggccggcg ggacggcgaa
      541 acagggccgg gaccggcgct tccaagcgat tctgccgctg aagggcaaga ttctgaacgt
      601 cgagaaagcg cgctacgaca agatgctcgc gcacgaggag atccgcgcca tgattacggc
      661 gctcggtact ggcattgcga aggatgactt cgatccgacg aaggtacggt acgggaagat
      721 catcctgatg accgatgccg acgtggacgg aagccacatt cgcaccttgc tgcttacgtt
      781 tttcttccgg catatgcagg aactgattaa gcggggcaac gtgttcatcg cgcagcctcc
      841 gctgttctcg ctgaagaagg gcaagtcgca gcagtacatc aaggatgagc gggagttcgt
      901 gaaagtcatg gtgaagcgcg cggcggaagg cttgagcgtt cgctatggcg agggcgcggc
      961 caagatcgag ggcaaagatc tggcgcgctt catgaccgtg ctcgacgagt atctgggctt
     1021 cttcgacaag ctcgacaagc gggtgcgcaa cgagcgcgtg acggagctga ttccgaagct
     1081 cgacctggcc aagcgtgcgg attttgaagg cgagaagaaa gcggcgccca agaaaatcga
     1141 gcggctggaa cgggagatca agaagttgca gaaagacgaa ggcttcaaga gcgtcgaggc
     1201 gcgttttgac gaagagcata acttgtggga ggtgagcttc gtcaacaagc atggcgccga
     1261 gcacgtcatc aactgggaac tggcgtcgag cgcggagtgc cggcagatga tctcgaagta
     1321 caagcagatc gaggcataca tggagcctcc gtttgtggtg gagacgctgg tcaaggccgt
     1381 ggctgcgggc aacaacgggg agggggcaga cggcgaggct gagacggggg aaggcgagtc
     1441 tttgggcaaa gaaaaggccg agaagaagac ggccaagccc gcggccaaac gcaaagctga
     1501 aatcgaagtt gtggagaaga cgaacgtccg cgatttgcgc gactatgtga tcaacgaggg
     1561 ccgaaaagac ttttcgatcc agcggtacaa gggcctgggc gagatgaccg cgccccagct
     1621 gtgggagacg acgatggatc cggagcggcg cacgctgttg tcagtgaagc tcgaagacat
     1681 cgccgagtgc gagaccatct tcaccacgct gatgggcgag gacgtggagt cgcggcgcaa
     1741 gttcatcgaa gaaaacgcgc tagatgtgaa gaacctggac atctgacatc taacgtcttt
     1801 cgccacggat cagcgcggat ttagacggat aaagtaatca agtatcggcg gccttcaggc
     1861 cgcttttttt ttgtcgcgga gaaattgcgg tctgtatcga tggagcaatt tagaatggcc
     1921 gcactatgca gggcttccga tccttcgtgc agcttttcct ggtgctgatc gcagctacgc
     1981 tctcactttc ccctgacgac tcttccgccg ctcccacgcc gccgatgggt tggaatagct
     2041 gggactccta cgggacaacg gttcgcgagg agcaagtgaa ggccaatgcc gactggatgg
     2101 cagagcacct ggcgaaatac ggctggcagt acattgtggt cgacatccag tggtatgagc
     2161 ccaatgctca ggggcatgag tataagcctg gagcaccgct caccatggac gaatacgggc
     2221 ggctaatacc ggcggtgaat cgttttccct cgtccgctaa tggcgcgggg ttcaagccgc
     2281 tggcagacta tgtccacggc aagggactta agtttggcat tcacatcatg cgggggattc
     2341 cgcggcaggc cgtggagaag agtctcccga tcaagggaac gagcttccat gccgccgata
     2401 tcgcggaccg caacaacccg tgccggtgga attcggacat gtggggcgtg gatacgacta
     2461 aatccggagg gcaagcttac tacgattcga ttgccgagtt atacgcgtcg tggggtgtgg
     2521 acttcatcaa agcagacgat atggggagcc atcggtttca gccggaggag atcaaggcgc
     2581 tgagcctggc tctgcgcaag accgggcggt cgatcgtgtt gagcatctca cccgggccag
     2641 cgtcgctttc cgatgccgag ttcttcgaaa agtatgcgca catgtggcgc atctcggacg
     2701 atttctggga tgactggaag ctgctgcgcc ggcagttcga ctacatgcgc gcttgggcac
     2761 cgttcgtggg gaagaacgac acttggcccg atgcggacat gctgccaatg ggcaagctgc
     2821 gggttacggc gacggaaggc gggggtacgc cgacaaagtt cactccggat gagcagcaga
     2881 cgatgatgac gctgtggagc atctttcggt ccccgctgat tctcggaggc gacctgccga
     2941 gtaacgacga cgctacaacg gcgctcatca ccaatcccga ggtgctcgac gtcgaccaac
     3001 acagcagcgg cagacacgag gttctcgtaa aaggaaactt gcgagtgtgg gtggcggatg
     3061 gagcgaagcc gggtgagcga tatgcggcgg tgttcaactt gggtgagagt gccgggccgg
     3121 tctcgcttga ctggagggag gttggaattc agagtccggt cgtgcaagtg cgggacttgt
     3181 gggcgcacaa aatgctgggt gaagtggcgg gcattcatgc cagcctcagg ccccatgcaa
     3241 gcctgctgta tcgggtgatg gtttccgaga gccactgact gaacatcaag gacatcgcac
     3301 acggcaaaca cttgctacgg gacggtgcgg gctaagagca ggtccctcgt cgctgcgctc
     3361 ctcgaatgac aacagttttt gggcttgctg gtttgctggg ctaatctagc cgaaatctat
     3421 ttcaatcctt ccactaagta taaatccgcc agcatgcggt ggtagccgaa gacgcaggtc
     3481 ttggcgtcgg gggtcatgag gatggggccg atgttctcga cgccggcagg gtcggagggc
     3541 atgagttctt tccagagggt gcgctggccg gtctgcagat cgacgtggta gacacgtgca
     3601 gggagttctc ccggctggta cacgtagagg gagcggccat cgcccgccca ggtgaccggc
     3661 tgctctccct ggttaaagcc agcgatggga cgcggctcgc cgccagcgac gggatagaga
     3721 tatcccttct ggtcaggacc gatggcggcg atctgctggg aatcgggtga aatgctgaaa
     3781 gcggtgccgt tcactccttc cggagaaatc gcctgactct tgcccgatgc catgtcgtag
     3841 acgtaaaagc gcacgccctt gccgggttca ttcgctgaaa aaagaacgcg cttgccgtcg
     3901 ggaaaccagc gcgcccagct atggttgata ttgtctttgg tgagctcgcg aggttcgccg
     3961 gcgcctgtgg gcagaatctt gaattgcgac ggcgagtcct gggtttggga gatcgcccac
     4021 ttgccatcgg gggaaagggc tacggccccg ccttcgccca gcaggactgc aggtgtgccg
     4081 tccgtcttgc gaatgtagac cgcgtaggtg aggccgctgg atttcgagta atcgagcgca
     4141 ccgccgccgc cttcttcgtc gaagagcaga gttctcccgt cggctgagag atcggcggga
     4201 taggtgtagt cgagccagga aagttcgtgc tgcttggggt cgctggtaaa gaggccgagc
     4261 aattcgcggc gccagtcggc gcggacgaga agcacgcgac cgtctttggc gatgtcgaga
     4321 aggatcagcg cgcccggaag gcgagccacg atgcgctgcg
//
LOCUS       DASPUL010000282         3792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1963979, whole genome shotgun sequence.
ACCESSION   DASPUL010000282 DASPUL010000000
VERSION     DASPUL010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1963979"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2398
                     /gene="ppdK"
                     /locus_tag="VEJ00_06920"
     CDS             <1..2398
                     /gene="ppdK"
                     /locus_tag="VEJ00_06920"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HXX00929.1"
                     /translation="NDKSVEALARRSNNPRFAADSYRRLIQMFGNVVLDIEKSAFDEV
                     FEAKKKQKKAKLDTDLDAKALQEVIAEYKKVVKKHAKRDFPQDPREQLVMARDAVFRS
                     WQNERAKHYRRINNIDDMLGTAVNVQAMVFGNLGETSGTGVGFTRNPATGAKEFYGEF
                     LMNAQGEDVVAGIRTPVHISELHKIMPDVYDQLRDITTRLEKHYRDMQDFEFTIQDGK
                     LYMLQTRNGKRTGLAAVRVAIQMVEEGLITKEEAIFRVEPNQLYDFLVPRLDEKSTKV
                     EVLATGLPASPGAAVGQIVFTADEAVKKAGHGAKKNPVILVRGETTPEDIQGMEVATG
                     ILTSRGGMTSHAAVVTRGMGKCCVAGAGDISVDEKKREMRVKGQTFKEGDWISLDGTT
                     GRVIKGKLNTIPPKADDPELMQLMGWAEPFRKLRVRANADIPRDAVQARAFGAEGIGL
                     CRTEHMFFGEKKIPHMRAMILADNEKDRRAALKKLLPMQREDFAGVFKAMDGLPVTIR
                     TLDPPLHEFLPRREELMVDIALLARYSAKQKKELVTKYKDYGVKGAGDLRKVLPALLA
                     RVEQLHEFNPMLGHRGCRLGITYPEITEMQARAIFEAAIDCTKKGIKVHPEVMIPLVA
                     TLKEMANQGAIVRRVAEDVFKEKGMRVDYMVGTMIELPRAALVADEIAKEAEFFSFGT
                     NDLTQTTFGFSRDDVNKVLPTYLAEGILKQDPFAALDQEGVGQLVKMATERGRKTRPN
                     LKVGICGEHGGEPSSVEFCHRVGLNYVSCSPFRVLTARLAAAQAAATETLKVEGGRTK
                     "
     gene            complement(2466..3059)
                     /locus_tag="VEJ00_06925"
     CDS             complement(2466..3059)
                     /locus_tag="VEJ00_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00930.1"
                     /translation="MPDPLYLSLWFPDFSGPAMLPHVLAVLQQFPSSRQRPGIAYVAV
                     QPVSWNEATVLERRFSPGIEPAEALVVAADLVHDDYAYVLDAYWDLWTPDETAQQWRL
                     VPNLVKFIVQGEEFDEGAAERTGHIQIDFGLDSPFLQENVELTDEAQAKVRENVSKLV
                     EFTIKGEKNTRATGRLLWSESDENLAQKLIARLQKVH"
     gene            3214..3291
                     /locus_tag="VEJ00_06930"
     tRNA            3214..3291
                     /locus_tag="VEJ00_06930"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3250..3252,aa:Glu,seq:ttc)
BASE COUNT          851 a         1012 c         1216 g          713 t
ORIGIN      
        1 gaacgacaag agcgtcgagg cactggcaag gcgcagcaat aatccgcgat ttgcggcgga
       61 ttcctaccgg cggctcattc agatgttcgg caacgtggtg ctcgacatcg aaaagtcagc
      121 gttcgacgaa gtgtttgaag ccaagaagaa acagaagaag gcgaagcttg acaccgatct
      181 cgacgccaag gccctgcaag aagtgattgc cgaatataag aaggtcgtga agaagcacgc
      241 caagcgagac tttccgcagg atccgcgcga acaactggtc atggcgcggg atgcggtgtt
      301 ccgctcgtgg cagaacgagc gggcgaagca ctatcggcgc atcaacaaca tcgacgacat
      361 gctggggacc gcggtcaacg tgcaggccat ggtgttcggg aatctcggcg agaccagcgg
      421 cacgggcgtg ggctttacgc gcaatccggc gacgggcgcg aaggaattct atggcgagtt
      481 cctgatgaac gcacaaggcg aagatgtggt tgccggcatc cgcacgccgg tgcacatctc
      541 cgagctgcac aagatcatgc cggacgtgta cgaccagcta cgcgacatca cgacgcggct
      601 ggagaagcac tatcgcgaca tgcaggactt cgagttcacc atccaggacg gcaagcttta
      661 catgctgcag acgcgcaacg gcaagcgtac ggggctggcc gcggtgcgag tggcgatcca
      721 gatggtagaa gaaggcttga ttactaaaga agaagcaatc ttccgggtcg aaccgaacca
      781 gctatatgac ttcctggtgc cacgtctgga tgagaagagc acgaaagtgg aagtgctggc
      841 gacgggtttg ccggcttcac caggtgcagc cgtggggcag atcgttttca ccgcggacga
      901 ggcggtcaag aaagcagggc acggggcgaa gaaaaatcct gtgattctgg tgcgcgggga
      961 gacgacgccg gaagatattc agggcatgga agtggcgact ggaattctta cttcgcgcgg
     1021 cggcatgacc agccacgccg cggtcgtcac gcgcggcatg ggcaaatgct gcgtagccgg
     1081 agcaggcgac atttcggtcg acgagaagaa gcgcgagatg cgcgtcaagg gccagacgtt
     1141 caaggaaggc gactggattt cgctcgacgg cacgacgggc cgtgtgatca agggaaaact
     1201 gaacacgatc ccaccgaagg cggacgatcc cgaactgatg cagctgatgg gatgggcgga
     1261 gccgttccgc aaactgagag tgcgcgccaa cgccgacatt ccccgcgatg ccgtgcaggc
     1321 gcgggcgttt ggtgcggagg gcatcgggct gtgccgcact gagcacatgt tcttcggcga
     1381 gaagaagatt ccacacatgc gggcgatgat tctggcggac aatgagaaag accggcgggc
     1441 agcgctgaag aagctgctgc cgatgcagcg ggaagacttt gccggtgttt tcaaggcgat
     1501 ggacggattg ccggtgacga tccgcacgct cgatccgcca ttgcacgagt tcctgccgcg
     1561 gcgcgaagaa ctgatggtcg acatcgcatt gcttgcgcgg tatagcgcaa agcaaaagaa
     1621 agaactggtc acaaaataca aagactacgg tgtgaagggc gcgggcgatc tgaggaaagt
     1681 cttaccggcc ctgctggcgc gcgtggagca gttgcacgaa ttcaatccca tgctcggtca
     1741 tcgcggatgc cggctgggga ttacctatcc tgagatcacc gagatgcagg cgcgcgccat
     1801 cttcgaggct gccatcgatt gcacgaagaa gggaatcaag gtgcatcccg aggtcatgat
     1861 tccgctggtg gccaccttga aggaaatggc gaaccaaggc gcgatcgtgc gacgcgtggc
     1921 cgaggatgta ttcaaagaga agggcatgcg cgtcgattac atggtcggaa ccatgatcga
     1981 gctgccccgc gctgctctgg tggcggacga aatcgcgaag gaagcggagt tcttttcgtt
     2041 tggcacgaac gacctcaccc agaccacctt cggattctcg cgcgacgacg tcaacaaggt
     2101 gctgccgacg tatctggcgg aggggattct gaagcaggat ccgttcgccg cactcgatca
     2161 ggaaggcgta ggacagttag tgaagatggc gaccgagcgt ggccggaaga cccgtccgaa
     2221 tttgaaggtg gggatctgcg gcgagcacgg cggcgagccg tcgtcggtcg agttctgcca
     2281 ccgcgtgggg ctgaactatg tttcctgctc gccattccgc gtgctaaccg cgagactggc
     2341 ggcggcgcag gcggcggcga ccgagacgct gaaggttgag ggcggaagga cgaagtagcc
     2401 taaccgaacc aaacgggaat atggagggga cagaagccct ccttcttcgc ctttgtcctt
     2461 cgtgcttagt gcactttctg caggcgcgcg atcagtttct gcgccagatt ctcgtcggat
     2521 tctgaccaca gcaatcggcc tgtggcgcgc gtgtttttct ctccctttat ggtgaactcg
     2581 accaacttcg agacattttc gcgcaccttc gcctgagctt cgtcggtgag ttcgacattc
     2641 tcctgaagaa acggcgagtc gagaccgaaa tcgatctgga tgtggcccgt ccgctccgcc
     2701 gcgccctcgt cgaattcttc tccttggaca atgaacttca cgaggtttgg aacgagtcgc
     2761 cattgctggg cggtttcgtc cggcgtccag agatcccaat aggcgtcgag gacgtaggca
     2821 tagtcgtcgt ggacaagatc ggcggcgacg accaaggcct ctgccggctc aatgccggga
     2881 gaaaagcggc gctcaagtac ggtggcttcg ttccaggaga cgggttgcac ggcgacatag
     2941 gcgatgcccg gcctttgcct ggaagaaggg aactgctgca gaactgccag cacgtgcggc
     3001 agcatggccg ggccggagaa gtcgggaaac cagaggctca gatagagcgg atcgggcata
     3061 gctgggcgag tcgttgactg acatttattg tatgcgatcg agggagccgg aaccgcagcg
     3121 tgggtgtgaa atgcactcgg ggcgaggtgc attttgacag cccggaacgc cgctgttacc
     3181 atgacatttc agcagccatc ctgcctgagt gaagtccccg tcgtctagcc cggcctagga
     3241 catcgccctt tcacggcggt aacacgggtt caaatcccgt cggggacgcc aaaccctttc
     3301 agtgacttgc gggcaattgc cgaaattgtc gtaggcacaa aagggcacaa ctctaaccag
     3361 agtcccgcga gcgtctctgc tgaaaaatca gtctttccgc tcgtttccgg gtgtttttca
     3421 taggcacaaa aaggaacagc caagactccg atgcgttcag cctgcagttg cactcgagga
     3481 acgcaacagc cttatgacat cactctgagc ttttcgtttg tgaagcgtta ctgcctgcgt
     3541 ataagtgtcg agcgtgaccc tgctggacgc gtgacggagt agttcttggg tgacttttat
     3601 atccgtcctg ttctgtctta ggagagaaga atacgtgtgg cggaaagtgt gccagccgat
     3661 cggtttcaaa atcccgacct tcaacgcggc tggccggata aaagttcgca tgatgcactg
     3721 gccccaataa gggtttcggc cccgagcagc acgactcgcg aatacccagt catccggtct
     3781 tttgaatcgt gt
//
LOCUS       DASPUL010000283         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_322572, whole genome shotgun sequence.
ACCESSION   DASPUL010000283 DASPUL010000000
VERSION     DASPUL010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322572"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VEJ00_06935"
     CDS             <1..539
                     /locus_tag="VEJ00_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00931.1"
                     /translation="LLVGYISFYARYTYWSGDFAWGDRYVSTAVEMAALLAVPLLLRH
                     RADLSLWIWRGGVLFIAASLLIQIASLAFWLPLEIYQMETFGHPTLVIALRFKNIAAF
                     ALGKMEAWGLNTETLTQDPWDYVHITTWNFLPFLLRRVGAAPGWVVNVVFAVWYAAIT
                     ALATVLWRLRTVLRAPLD"
     gene            complement(570..1106)
                     /locus_tag="VEJ00_06940"
     CDS             complement(570..1106)
                     /locus_tag="VEJ00_06940"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXX00932.1"
                     /translation="MQAKSSLAAPTRRQMMSAVAVTMGSLAAVPRLLGQPQQNMMETP
                     STGAEGLLTYLHQEVEVKAAAQRVYEALLDSKQFAAFTGAPADISRDAGGTFSLFGGL
                     IVGRNVELVLHQRIVQAWRPSHWEPGVYSMVKFELRERGAQTRIVLDHTGFSEGNFRH
                     LNAGWNEHYWEPLKNFLA"
     gene            1250..1891
                     /locus_tag="VEJ00_06945"
     CDS             1250..1891
                     /locus_tag="VEJ00_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXX00933.1"
                     /translation="MPYPAGHRNTMRARIIDSARRLFNRHGFDNVSIHQIMSGAGLTH
                     GGFYTYFRSKSDLYTEVLGCFFTDPNWKNCWEGVEVDLSSEDVGPQVVRAYLSDQHFD
                     DVENSCPMVAVPSYVTRSSKSVRRVFERVFNAMVSVLERSLVEKGARRRETAQAAAAL
                     CIGGMIVARAVADPDLANELRAACMAVALQLGGWDRPVNGRNGVKAARLQQAV"
     gene            2061..>2380
                     /locus_tag="VEJ00_06950"
     CDS             2061..>2380
                     /locus_tag="VEJ00_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX00934.1"
                     /translation="MRTRVLLSPAVLVSVPLCAYGAASPAGEYSTHFDALTKLSIAVA
                     QAMPADQYGFRPHPESMNFGDLMSHIATTNYQFCAGLKDTDTSQLPSPTDKDGIVKFL
                     SDSFA"
BASE COUNT          426 a          767 c          658 g          529 t
ORIGIN      
        1 tcctcctcgt gggctacatc agtttctacg cgcgctacac ctactggagc ggtgacttcg
       61 cctggggcga ccgctacgtt tcgaccgctg tggaaatggc ggcgctcctc gccgttcctc
      121 tgctgctgcg tcatcgcgcc gatttaagtc tctggatttg gcgcggagga gtgctattca
      181 tagccgcgag tctcctcatt cagatcgcct ctcttgcttt ctggctcccg ctcgagatct
      241 accagatgga aaccttcggt catcccacgc tcgtgatcgc gctgcgcttc aagaacatcg
      301 ctgcctttgc cctggggaag atggaggcgt ggggcttgaa cacagaaaca ctgacccagg
      361 atccctggga ctacgtccac atcacgacct ggaatttcct gcccttcctg ctgcgccggg
      421 tcggggcggc tccgggttgg gtcgtgaacg ttgtcttcgc agtctggtac gccgcgatca
      481 cggcattggc cacggtcctg tggaggctac ggaccgtact ccgagcaccg ctggattgag
      541 gtcttcccca tgtcgaccgc ccgggaaatt caggccagaa agttcttcag cggctcccag
      601 tagtgctcgt tccatcccgc gttcaggtga cggaaattgc cttcggagaa gccggtgtgg
      661 tcgaggacga ttctcgtctg tgctccccgc tccctgagtt caaacttcac catcgaatag
      721 acgccgggct cccagtgcga cggccgccag gcctgcacga ttcgctggtg aagaaccagt
      781 tcgacgttcc gtcctacgat caagcctcca aataaggaga atgtcccgcc cgcatcgcgg
      841 ctgatgtctg ccggcgcacc cgtgaacgcc gcgaattgct tggagtcgag cagcgcttca
      901 taaacgcgtt gcgctgctgc cttgacctca acctcctgat gcaagtaagt aagcagtccc
      961 tccgcgccag tgctgggcgt ctccatcatg ttctgctgag gctgccccaa caatctggga
     1021 accgccgcca gacttcccat cgtcactgcc acagcgctca tcatctgccg ccgcgtcggc
     1081 gcagccaggc tcgatttcgc ttgcattctt cctcccatcg acctcggaat tgttccaccg
     1141 ccttcgcatc agaccatcca ggcaaaatat gttcaagata atatttcgcc tggcatttgc
     1201 gccgcgttcc gggcatactg ccctgcatca tcctggggag gacgccctga tgccttatcc
     1261 cgccggccat cgcaatacga tgcgggcccg aatcatcgac agcgcccgcc gtctgttcaa
     1321 ccgtcacggc ttcgataatg tttcgatcca tcagatcatg tccggtgctg gcctgaccca
     1381 cggcggcttc tacacgtact tcaggagcaa gagcgatctc tacactgagg tcttaggctg
     1441 cttctttact gaccccaact ggaagaactg ctgggaaggc gtggaagtgg atttatcgtc
     1501 agaggatgtg ggtccgcaag tcgtccgcgc ctatctgtcg gaccagcatt tcgacgacgt
     1561 cgagaactcc tgccccatgg tcgccgtgcc ctcgtatgtg acccgcagca gcaagagcgt
     1621 gcggcgcgtt ttcgaacggg tgttcaacgc catggtcagc gtgctcgagc gcagcctggt
     1681 cgaaaagggg gcgcgccgcc gcgaaaccgc tcaggcggcc gcggctctgt gtatcggcgg
     1741 aatgatcgtc gcgagggcgg tcgccgatcc ggatctggcc aacgaactgc gcgccgcttg
     1801 tatggcggtc gcactgcaac tcggcggctg ggaccggccc gtcaacggca ggaatggtgt
     1861 caaggcagct cgtttgcagc aggccgtcta actgtcccgt cggtagctcc gaagtcctgg
     1921 caggatagga gctaatttgg ccttctccgt cggttgacct aagtgaaggc tatgcgtcca
     1981 tcagtggaag taacgccttc ccaatgcccc accgcacttg tgtggccagc tggggaaact
     2041 gcatccgctt aggagcttct atgagaactc gcgtgctgct atcgcccgcg gttttggttt
     2101 ccgttcctct ctgtgcttat ggcgccgctt ctccggcggg cgaatactcc acccatttcg
     2161 acgccctcac caagctctcg atcgcagttg cccaggccat gcccgccgac cagtacggtt
     2221 tccgtccgca tccggaatcc atgaatttcg gcgatttgat gtcccacatc gccaccacga
     2281 attaccagtt ctgcgcaggt ctcaaggaca ccgatacttc ccaactgcct tcccctaccg
     2341 acaaagacgg catcgtcaaa tttctgagcg attcgttcgc
//
LOCUS       DASPUL010000284         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1964312, whole genome shotgun sequence.
ACCESSION   DASPUL010000284 DASPUL010000000
VERSION     DASPUL010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1964312"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VEJ00_06955"
     CDS             complement(<1..910)
                     /locus_tag="VEJ00_06955"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00935.1"
                     /translation="MLRIGGQKVPTPLLLLLAVDCLLIAFGLVIATGLRLGVNGLPYV
                     MHYLGNWQTLLRFLLVIAVCEVSLYFNDLYDFRLMATRNEILVRLLQAFGIACVGLAF
                     SYYLAPDLGFGRGIAALAAPLIVAMTLGWRLLLSQGPHALGAAERMLIMGTGPTGISL
                     ARVILSRPELQLKVVGFLDEKGQDIGKSLVNPGIIGAAEDVEDVVRKEKIDHVVISLL
                     ERRGRMPVRQLLHLKFAGVKVEDAHAFYERMTGRIILERLSPSWLILSDGFRKSMLLV
                     WAKRAIDVVVSAFALALCTPLFIITAV"
     gene            1508..>2019
                     /locus_tag="VEJ00_06960"
     CDS             1508..>2019
                     /locus_tag="VEJ00_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00936.1"
                     /translation="MPIEIRSADLESDRAALVELMRQALTPESDVERFEWLYRRGPHG
                     AARAWLAVDGGTRQPVGAAAAFPRRMWWDGKERSAFVLGDFCMLEKYRSLGPSLALQR
                     ACMNGIANGAEAFFYDFPSQGMMAVYKRLGIAPAGELVRWALPLRSGAKLEALTRSKK
                     VARGLAPLAD"
BASE COUNT          440 a          590 c          625 g          364 t
ORIGIN      
        1 ccacggcggt aatgatgaag agcggcgtgc acaaagccag tgcaaacgcc gaaacgacga
       61 catcgatcgc gcgttttgcc cacaccagca gcatggattt gcggaagccg tcggaaagaa
      121 tgagccagct tggcgaaaga cgctcgagaa tgattctccc cgtcattcgc tcgtaaaacg
      181 cgtgcgcatc ttccaccttg acgcccgcga acttgagatg caggagttgc ctcacgggca
      241 tccgcccgcg gcgttccaaa agagaaatca ccacgtgatc gatcttttcc ttgcgcacca
      301 cgtcttccac atcttcggcc gcgccgatga tgccggggtt cacgagagac ttgccgatgt
      361 cctggccctt ttcgtccaaa aatccgacca ctttcagctg cagttcgggt cgcgagagga
      421 taacgcgggc cagagaaatt cccgtcggcc cggtgcccat aatcaacatt cgctcggccg
      481 cgccgagagc gtgcggccct tgcgacaaca gcagccgcca gcccagagtc atggccacaa
      541 tcagcggggc ggccagggcg gcgatgcctc ggccgaatcc gaggtccggc gcgaggtaat
      601 aggaaaacgc aagacctacg cacgcaatgc cgaacgcctg caacaaccgc accaggattt
      661 cgttgcgcgt ggccatcagc cggaaatcgt acaagtcgtt gaaatacagg gacacttcac
      721 agaccgcaat cacgagcagg aaccggagga gcgtctgcca gttccccagg tagtgcatca
      781 cgtaggggag cccgttcacg cccagccgca gcccggttgc gatcacgaga ccaaacgcaa
      841 tcagcagaca atccacggcc agcaggagaa gcaatggcgt cggaaccttt tggccaccga
      901 ttctcaacat caggaggtac cctccgggat cttcggcacg aagtctttgc ggtcgcaggt
      961 ctgaggcacg cgcgacactc gttcactcaa gaggcgactc agcgacttgc gggtgcagct
     1021 ttcgaattct cagaacagga catgtgtgca cttggcagac caatgggact tgtcggagaa
     1081 ggcagggggt tcttcccgta agtcaatgtt cctaggccct ttagctggca gtaccgataa
     1141 ggccggaact gcgcactcca taaggaccga ctttgcatag tccaaccggc ttttattgag
     1201 taagttagag acgaaaagga gtagaccggg ctggagtcgc ttgccgctta ttcgccttaa
     1261 gaaagaaaac attttcctac tctcgccatc accggaaggc aaagaatgga attcgcccgc
     1321 ggaaaagagc actaccggcg ggcaccagtg agtccgcgtc gtaaccagct gtgtgtcagt
     1381 aggttagacg gctcgccgga ggtcccgtct ggcagaacac ttgctcggat agggagtgga
     1441 tcgtctgcgg ccgacgtcat actttgccaa gagcggcgct cttccaagcc cgattgagtt
     1501 tgtcgaaatg cccattgaaa ttcgttccgc agacctggag tccgacaggg cagcgctggt
     1561 tgaactgatg cgccaggctc tgacgccgga gtcggacgtg gagaggtttg aatggcttta
     1621 ccgccgaggc ccgcacggcg cggcgcgagc ctggctcgcc gtggacggcg gaacgcgaca
     1681 gccggtggga gcggccgcgg cgtttccgcg caggatgtgg tgggacggaa aggagagaag
     1741 tgctttcgtg ctcggcgact tctgcatgct tgaaaagtat cgcagcctcg gaccgtctct
     1801 ggcgttgcag cgcgcgtgca tgaacggaat cgcgaacgga gcggaggcgt ttttctacga
     1861 tttcccgagc cagggcatga tggccgtgta caagaggctc ggcattgcgc cggcggggga
     1921 gctcgtgcgc tgggcgctgc cgctgcgctc cggcgcgaag ctcgaggcgc tcacccggtc
     1981 gaagaaggtg gcccggggac tggcaccgct cgcggactt
//
LOCUS       DASPUL010000285         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_457064, whole genome shotgun sequence.
ACCESSION   DASPUL010000285 DASPUL010000000
VERSION     DASPUL010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457064"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1043
                     /locus_tag="VEJ00_06965"
     CDS             270..1043
                     /locus_tag="VEJ00_06965"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXX00937.1"
                     /translation="MPSRAKTRKIGLARALSKLGYCSRSQATGLICAGRVCLNGSVRR
                     DPETPVALERDRLLVDGAGISQQARVYLMMNKPRGLVTTASDEKGRDTVYSLLTGACL
                     PWVAPVGRLDKASEGLLLLTNDSEWAARVAAPETHLEKTYHVQVGSLAGEELVEALIS
                     GVRSDEGDLLRVRRASLLRSGEKNCWLEIILDEGKNRQIRRMLAALGVEVLRLVRVAV
                     GSLQLGNLAKGEYRPLTPGEKASLDRGMETYQGRPAPHG"
     gene            1036..>2145
                     /locus_tag="VEJ00_06970"
     CDS             1036..>2145
                     /locus_tag="VEJ00_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXX00938.1"
                     /translation="MADITFLSAASMVEQIRKKEISPVELAQAHLAKIERLNPRLNAF
                     VHVDAERVRYEAHAAEKAVMNGEALGALHGIPVSIKGSIDVAGLRCESGTRLRAGNVA
                     QNDAPLVKRLRAAGAIVLGVTNTPELLMAWETDNLLYGRTNSPWDLERTPGGSSGGEA
                     AAIAAGMSAGGVGSDGGGSIRVPAHFSGICGLKPTPGRIPATGHFPASGGPFALIGVV
                     GPMARTVPDLKMLLEVMQGPDDGDACAAPVPARGPSDKEVRMLRIGYFEDDGHTPVTP
                     EIRAAVRAAAEALRSAGFQVEPFRPEGLEEARGLWTKFFVKVAGMLIQPMFAGREHDQ
                     SPILKQFLEWSAAEPELTGGSLLEAWIRRDTVRAEF"
BASE COUNT          399 a          588 c          761 g          397 t
ORIGIN      
        1 tctcctcgcg cacgtcgacc agcaagaact tgtcgccgtg gtccatgcgc ttcttgactt
       61 cgtcgacatt ggtctcgcgc acgcgagtcc tggcgtcgtc aacgatcttg aggaagcggg
      121 gaggatgctg gtgggccatg gaggatctcc tttgcgcgac tgccgagtac ttcttccgcg
      181 cacttttcga tgaacatggt accaagcccg gtcgtgttgt gcccagaacg gcggccgaga
      241 gctcgacgcc tttcaggtag catggagcgg tgccatcgcg ggcgaaaacg agaaagatcg
      301 gcctggctcg ggcactgtcg aagctcggat actgctcgcg ctcgcaggcg accgggttaa
      361 tttgcgccgg gcgtgtctgc ttgaacggga gcgtgagacg cgatccggaa acgccggttg
      421 cactggagcg cgaccgcctt ctcgtggatg gagccggtat ctcgcagcaa gcgagggtct
      481 atctaatgat gaacaagccg cgggggcttg tgaccacagc ttccgatgaa aaaggccgcg
      541 acacggtcta tagcctgctg acaggagcat gtctgccctg ggtggctccg gtcgggcggc
      601 tcgacaaggc cagcgaagga ctgctgctgt taacgaatga ctccgaatgg gcggcgcggg
      661 tcgccgcccc ggaaacgcat ctcgaaaaga cttatcacgt gcaggtggga tcgcttgccg
      721 gggaagagct ggttgaggct ttgattagcg gcgtgaggag cgacgaagga gacttgttgc
      781 gggtgcggcg ggcaagcctg ctgcgatcgg gcgagaagaa ctgctggctc gagatcattc
      841 ttgacgaggg caagaaccgg caaattcgcc gcatgctggc agcactggga gttgaagtgc
      901 tccgattggt gcgcgttgcg gtcggttctc tgcaactggg caacctggcc aaaggggagt
      961 atcgcccgct gactcccggc gagaaggcat cgctggatcg cgggatggag acataccagg
     1021 ggaggcctgc cccgcatggc tgatatcact tttctttctg ctgcgagcat ggtcgagcag
     1081 attcgaaaga aggagatctc ccccgtcgag ctggctcagg ctcatctggc gaaaattgaa
     1141 cgactgaatc ccaggctcaa cgcgttcgtt cacgtggatg ccgagcgcgt acggtatgag
     1201 gcccacgcgg ccgaaaaggc cgtgatgaac ggagaagctc tcggggcact gcacgggatt
     1261 cccgtcagca ttaagggttc gatcgacgtg gccgggcttc gttgcgagtc ggggacgcgg
     1321 ctgcgcgcgg gaaatgtggc gcagaatgat gctccacttg taaaacgtct gcgcgccgcc
     1381 ggcgcgatcg tgctcggcgt gacgaatacg cccgaactgt tgatggcgtg ggagaccgac
     1441 aatctgcttt acggccgcac gaacagccca tgggatctcg agcgcacgcc gggcggctcg
     1501 agcggaggcg aagcggcagc gatcgcggcg gggatgtctg ccgggggagt gggtagcgat
     1561 gggggcgggt cgatccgggt gcccgcacac ttcagcggca tttgcgggct gaagcccacg
     1621 ccgggacgga ttccggcaac cggccacttt cccgcgtcgg gcgggccgtt tgcgctgatc
     1681 ggagtggttg gaccgatggc gcgtacggtg ccggatttga agatgctgct tgaggtcatg
     1741 caagggcccg acgacggcga cgcctgcgcc gctccggtgc cggcacgcgg gccgagcgac
     1801 aaagaagtca ggatgctgcg catcggctac ttcgaggacg atggccacac gccggttaca
     1861 cccgagattc gggcggcggt tcgagccgcc gccgaggcgt tacgcagcgc ggggtttcaa
     1921 gttgaaccgt ttcgtcctga agggctggaa gaggcgcgag ggctgtggac gaagttcttt
     1981 gtcaaagttg caggaatgct gattcaaccc atgtttgccg ggcgcgagca cgatcagagt
     2041 ccgatcctga aacagtttct ggaatggtcg gccgccgagc ccgagcttac gggagggtcg
     2101 ctgctcgagg catggattcg gcgcgacacg gttcgggcgg agttt
//
LOCUS       DASPUL010000286        13953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1828250, whole genome shotgun sequence.
ACCESSION   DASPUL010000286 DASPUL010000000
VERSION     DASPUL010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 13953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13953)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13953
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1828250"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1399)
                     /locus_tag="VEJ00_06975"
     CDS             complement(80..1399)
                     /locus_tag="VEJ00_06975"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXX00939.1"
                     /translation="MQSISLRPAKYSVLGVGFAARAAGIAASPLQVLMAAPTLLFLLT
                     LMAMLLRHPDVPFYPIDRVAFGLLLLAVAGRAMIARDRLFIERATWPMIGLALMALVS
                     VAGQPFDNEAASVLAAKVIVPFTLFHLAGLVFDRERRLRRFEIFAVVVLAYLTFTAIA
                     FLLGANWLIFPRFILDESVGIHADRARGPMLQAVANGVSINLLGLLALHAWRRGSMRG
                     AKAVLLLASVPIAILATMTRAVWLSFAGTVVVLVFRSKSCTRRRVCVAFVLLVGVALA
                     ALVSSSKLGEALGDRFEERGPVDYRQAVLAGGWQMFLERPLLGWGFHQMPAELARCVR
                     AYDEKVLYPHNTYLELLVEEGLVGLALYLWLMWELLRLGRAAIPAAEHDGFLNLQFHR
                     LWPVLLAVYWLNAAFVVMNYQFVNGLLFTLAGMLAAQQRRAESAQPC"
     gene            complement(1484..2932)
                     /locus_tag="VEJ00_06980"
     CDS             complement(1484..2932)
                     /locus_tag="VEJ00_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HXX00940.1"
                     /translation="MRGARKPPHTVRPREVAGEKVSEIPALPADPHDGALALLVPVPL
                     QGRMAWSWLRSIAADFVLVGLNWLLLGGLLVPLRSAFPRVRLFEYAAGAPASLLGIAV
                     LHAALITLMGYTEGLYASSSDLRGKARILAKSVFWTTTLLCFAYCLQGAAWMVGSLFC
                     AAGLLHFASLCTWRWHKQRQERDPGRSARDLRNALIVGAGHLGRRVAADVAAHREACR
                     RVCGFLDNDAPLGDGVVGRVTDLARLARRGFVDEVILAAPHDRNLTLNVLREARRLRL
                     DVEIVPELFGCKPGAEVERVGDLPMISLHAERWPAASLALKRLADIAGAGAMLTVLSP
                     LLLVIALLIKLGSPGPVLYTAMRAGRKARPFRCYKFRTMVRNADALKATLRQNNQRSG
                     PFFKIADDPRITRLGRFLRRYSLDELPQLWNVLRGEMSLVGPRPHPLDDFEGYDIEHL
                     ARLDVTPGMTGLWQVTARRDPSFLPARDGTGP"
     gene            complement(3033..3560)
                     /locus_tag="VEJ00_06985"
     CDS             complement(3033..3560)
                     /locus_tag="VEJ00_06985"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HXX00941.1"
                     /translation="MSAALSWPPQPVPEAWFAVQTRYRFEKKVAGKLSTKGLQVFLPL
                     RKENHAWSDRNKVVMVPLFPGYAFVHSARSLAARLLILQTPGVMGFVSFAGAAAVVPQ
                     KQIEDLQRLLAEEVPFSFYPFVQLGRRVRICGGCLDGVEGLLLQREKDKLVISMESIQ
                     RSLAIEIQGYDVEMI"
     gene            complement(3657..4292)
                     /locus_tag="VEJ00_06990"
     CDS             complement(3657..4292)
                     /locus_tag="VEJ00_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00942.1"
                     /translation="MKQPERWTPENFAREQILGLVRQVFFSSAARPVRQVVLSAVEPE
                     TVVRHLCRQIGETLALETSGSVAVIGGSLHSMPDAQNRPEQTADAARCANMPLRRIAT
                     AVRGNLWLAPALERNGDPVTAASLHSYLGEIRREFEYSIVEARPVGESNETIAMAQFA
                     DGIILVLSAVHTRRATALRAKEVLEAAQARLLGTVLSDRSFPIPEAIYRRL"
     gene            complement(4394..5827)
                     /locus_tag="VEJ00_06995"
     CDS             complement(4394..5827)
                     /locus_tag="VEJ00_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00943.1"
                     /translation="MVLFRRRRVFVCVGAMVFAAALFYAFAGARYQAHMKLLVRPGRA
                     DAPASAQENAPLDLTRITVSEEELNSEVEMLRDEDILRKVVEENALGGRDWFHFLRLG
                     EGRAQRVERAARRLAKQIDVDPVKKTNLITVSYSSADPSRAASVLQSLAGAYVEKHAG
                     VHRPGGELLFFEQQTRESRRQLEEAQRSLVDFMTGRDVVEAAEERDRALLRLSDEDAL
                     YHQTRVEMAEAQQKVSELDALLAKLPERTTTQVRIADNPGVLTALKTRLQALQEQRIQ
                     LLTKFEPGHRLVQEVEEEITQVRTAIAAENLNPVRDETTDRNSHYEWVKAELQRSQVE
                     LKGLQARAAAAAVQESAYHSVARRLGRDAITQADLQSTEKAAEENYLLYVKKQEEARM
                     ADALDQQRIVNVAIAEPPVVPALPLFSTFAVLGVGLAAAGAAGTGAAFAVDYVDPAFR
                     TPDDVLLYLNAPVLASFPRNPSRSLLA"
     gene            complement(5921..6709)
                     /locus_tag="VEJ00_07000"
     CDS             complement(5921..6709)
                     /locus_tag="VEJ00_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HXX00944.1"
                     /translation="MKRVPIFARFIASALLLLNCASAAFAQEEGRKNLSDGKQASPSS
                     FTGAEQGSDSTAAAKPGKADGLGNPLLGGERRPLYRLAPSDVVEISFTVAPEFNQTLT
                     VQPDGYVTLKDAGMVAVQGLNLEEFAAAVQRAYQGYLHDPQVAVALRDFARPYYVVGG
                     EIARPGKYQLRTDTTVAEAVEIAGGFTSYSKHSQVILFRRVNDDLVETRLLNLKKMLK
                     QSGLKEDAHLQPGDLVFVPQNALSKISRFLTRPSVSMYTNPAQF"
     gene            7327..8046
                     /locus_tag="VEJ00_07005"
     CDS             7327..8046
                     /locus_tag="VEJ00_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXX00945.1"
                     /translation="MSKLRAATSNGLGVLVADSNRMQAQLLTRALRRRAEFRIATCRM
                     DTISILEAVAARVPQVALLALSSPTSLTETIMALRRFHLSHPEIPKVLLVDSDDRDVV
                     VSAFRSGARGILSITEANLRLLCKCILRVAEGQIWANTAQLKCLIDLISEVPSLRVLN
                     SRGDSLLTPREEQVVALVAEGLGNRQIAHELNLSEHTIKKYLFRIFEKLGISTRVELV
                     LYAVNHGQPRQAEWLAGTRSA"
     gene            8154..8537
                     /locus_tag="VEJ00_07010"
     CDS             8154..8537
                     /locus_tag="VEJ00_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00946.1"
                     /translation="MPEADSNPVPAPKTRRWTIVAAGLLLLALSLVVIYTSHRAFISP
                     LALVVVAAIGLAALLLQLRLRKDIRVPVRAPLLLNWLGLLFAMAAVVADFFHFGGGVM
                     IVVALGAVLCFAISGIFVLHALRKQ"
     gene            complement(8640..9536)
                     /locus_tag="VEJ00_07015"
     CDS             complement(8640..9536)
                     /locus_tag="VEJ00_07015"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXX00947.1"
                     /translation="MAAGEHIPAWNGPSRRRVPRYRLHSPLDVTVLRSGIPETVPGRS
                     VNVCEGGIAAMLAGELTPGEAVGLEVQLPLLSDPLRTRATVRYQENLRCGLEFVGLSA
                     EQRAAIRDWVKESKAEPEISALPVAESRDFRDEEGQKADRTRSANGSGRNNRSRGKKR
                     RGTVWIVFLFFLAVAAAVFWWKWNRAWEELESGLKSEETTAAEKPEAQVPAEVMEKLI
                     IHRVEPVYPAEARRANLQGIIALDIVVGRDGSVISTRALNGPEILARAAMDALRWWKF
                     EPYRLNGEPAVVETQVAVEFKR"
     gene            complement(9551..10228)
                     /gene="rpiA"
                     /locus_tag="VEJ00_07020"
     CDS             complement(9551..10228)
                     /gene="rpiA"
                     /locus_tag="VEJ00_07020"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HXX00948.1"
                     /translation="MANDQEKETAARASLRFIKDGQVVGLGSGSTATYFIKLLGEQVK
                     NGLRIRGIPTSVRSRDLAMSLGIPLTSLDECQDIAVTVDGADEVDPHLRLIKGGGGAL
                     LREKIVASATRQLVIVADASKQVPLLGKFPLPVEVIKFAQPLVAKRIRALGAEVQVRA
                     GEDGKPFLTDENNHILDCHFGEIRDADALARQLSDMPGVVEHGLFIGMASVVLLARGN
                     EIVELRR"
     gene            complement(10253..10636)
                     /locus_tag="VEJ00_07025"
     CDS             complement(10253..10636)
                     /locus_tag="VEJ00_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HXX00949.1"
                     /translation="MDNANPNRARDHLANERTFLAWVRTGAAIVVFGFAIGRFAIAMR
                     QLTEISGHPLRTAGLSVWIGAFSILLGVALVVAGLARYRKTRVQLDEGAFEPAGFLLS
                     LVTVLTALFGLVLAAYLIYTETSLR"
     gene            complement(10662..11357)
                     /locus_tag="VEJ00_07030"
     CDS             complement(10662..11357)
                     /locus_tag="VEJ00_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HXX00950.1"
                     /translation="MSRLLVLACGVWPVCCLVPPAAAQSGAPTAQSSQTLMIENASPL
                     AETYPGARYEVYFRARGGVPPLHWRAENGALPPGITLQDNGLLHGQIGRVGDFQFTAV
                     VTDSGQPQVTVKKLFVIHVHSALVLNWKSPARVNGNRIEGSAEVTNSTPDNIDLTFIV
                     LAVPENGRATAIGYQHFVLPAGTTKKELPFGETLPRGGYVVHVDAIGEVEAKNLIYRE
                     RMATPRPLTVAVE"
     gene            complement(11754..12512)
                     /locus_tag="VEJ00_07035"
     CDS             complement(11754..12512)
                     /locus_tag="VEJ00_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00951.1"
                     /translation="MTNSPRPGRLVILSGPSCVGKTPLHKSLAKFYPELSAQLRKLVL
                     FNSRRPRPGEVDGVDYHFRTRTQIESLQSDGRYVLIEVRSDLQALDAQELNSLLESGD
                     VLFEGSPFVGRVLQTHPRLTAVNRLSVFVSPLSREEIVYLKAPERNLSLPDFVTDVMR
                     RKLLRRTSRQKQELSAKDLEDIETRAGSAYREMKEAWRFQYVIANHDGEDSDNWEAFY
                     YPIGDARNSLETFAALLKGSIPSSLEKWDGDLLP"
     gene            complement(13162..13629)
                     /locus_tag="VEJ00_07040"
     CDS             complement(13162..13629)
                     /locus_tag="VEJ00_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXX00952.1"
                     /translation="MKISQKGLYALQAMMTLARRYDQGAIKIRDIAADSDLPEKFLEL
                     ILLELKNARIVDSVRGARGGYCLRRDPAEIPLSDIIRLIDGPLAPLGDAEQLRGLIAK
                     DADHRALYKVFLDVRDAAAKILENTTVADIAGMGRGKGATAKGTLGGKRSKQA"
     gene            complement(13837..13923)
                     /locus_tag="VEJ00_07045"
     tRNA            complement(13837..13923)
                     /locus_tag="VEJ00_07045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13887..13889),aa:Leu,seq:cag)
BASE COUNT         2683 a         4407 c         4184 g         2679 t
ORIGIN      
        1 cgcttccgca gttcgccgat ctcctccacc acgtaaggct cgactggaga aggaaatcga
       61 ttcgcgagat aggccacggt cagcatggct gagctgactc cgcccgccgc tgctgcgccg
      121 ccagcatccc ggcaagcgtg aacaacagtc cgtttacgaa ctggtagttc atcaccacaa
      181 aagcagcatt gagccaatag acggcgagca gaaccggcca aaggcggtga aactgcagat
      241 tgagaaaccc gtcgtgctct gctgccggga tcgcggcgcg ccccagacgc aacagctccc
      301 acatcagcca cagatacaga gccaggccca cgagcccttc ttcgaccagc aattcgagat
      361 aggtgttgtg gggatacagg actttctcgt cgtaggcgcg gacgcaccgc gccaactcgg
      421 cgggcatttg gtggaagccc catcccagga gcggacgttc gagaaacatc tgccagccgc
      481 cggcaagaac ggcctggcgg tagtcgactg gcccgcgttc ttcaaaccgg tcacccaatg
      541 cttctccgag cttgctggaa ctcaccaacg ctgccagcgc gacgcccacc agcagaacaa
      601 acgccacgca aacgcgccgg cgggtgcaac tcttcgagcg aaaaaccagg accaccacgg
      661 ttcccgcaaa cgataaccag accgcacggg tcatggtggc caggatcgcg atgggcacag
      721 aagccaggag cagcacagct ttcgcgcccc gcatgctgcc ccggcgccag gcatggagtg
      781 ccagcaggcc gagcagattg atggagacac cattggccac cgcctggagc atcggcccgc
      841 gcgcccggtc ggcatggatg ccgacgctct catcgagaat gaagcggggg aagatgagcc
      901 agttcgctcc aagcaggaag gcgatcgcgg tgaacgtgag gtaggccagc accaccacgg
      961 caaagatttc aaaccggcgg aggcggcgtt cccggtcgaa gaccagcccc gccaggtgaa
     1021 acagcgtgaa ggggacgatg actttggctg caagcacgct ggccgcttcg ttgtcgaacg
     1081 gctgcccggc cacgctcacc agggccatca aggcgagccc gatcatgggc caggttgcgc
     1141 gctcgataaa gaggcggtcg cgtgcgatca tggctcgtcc cgccaccgct aacagcagaa
     1201 gtccgaaggc cacgcgatcg atcggataga aaggaacgtc ggggtggcgc agcagcatcg
     1261 ccatcagcgt gagcaagaac aacagcgtgg gcgcggccat gaggacctgc agcggactcg
     1321 cggcgattcc cgccgcgcgg gcggcgaagc ccactccgag gacgctgtac tttgcagggc
     1381 gcaaggagat cgattgcatc agtcgccgct cccccgcacg accgctagca cagttttcag
     1441 caggatgcgc acatcggacc ccaggctcca ggtctggatg tattcacggt ccagtgccat
     1501 ccctcgctgg aaggaaggaa ggatcgcgcc gggccgtgac ctgccacaga cccgtcatgc
     1561 ccggggtaac gtcgaggcgc gccaggtgct caatgtcgta cccctcaaaa tcgtccaggg
     1621 gatgcggcct ggggccgacg aggctcatct cgccgcgcag cacattccag agttgcggca
     1681 gttcgtcgag gctgtagcgg cgcaggaagc gccccagacg agtgatgcgc gggtcgtcgg
     1741 cgatcttgaa gaacggcccg gagcgctggt tgttctgccg gagagtggct ttcaatgcgt
     1801 cggcattgcg caccatggtg cggaacttgt agcagcggaa gggccgcgcc ttgcgaccgg
     1861 cgcgcatcgc cgtgtaaagc acgggccctg gcgaccccag cttgatcaga agagcgatga
     1921 ccaacagcaa gggagagagc acggtcagca tagcgcccgc acccgcaatg tctgccagtc
     1981 gcttgagcgc aagactcgcc gccggccagc gctcagcgtg aagcgagatc atggggaggt
     2041 caccgacccg ttctacctcg gctcccggct tgcacccgaa cagttcggga acgatctcca
     2101 catcgaggcg caagcggcgc gcctcgcgca gcacgttcag tgtgaggttg cgatcgtggg
     2161 gcgcggccag gatcacctcg tcgacaaatc ctcgccgcgc cagccgcgcc aggtcggtga
     2221 cccggccgac gactccgtcc cccaggggcg cgtcgttatc gaggaagccg cagaccctgc
     2281 ggcaggcctc gcgatgggcc gcgacgtcgg cggcgaccct gcgtcccaga tggcctgcac
     2341 ccacgatcag ggcgttgcgg agatcgcgcg cggagcgccc gggatcgcgc tcttgccttt
     2401 gtttatgcca gcgccaggta cagagagagg caaagtgcag caagcctgcg gcgcagaaca
     2461 gactgcccac catccatgcg gcgccctgca ggcagtaggc gaaacagagc agcgtggtgg
     2521 tccagaacac cgacttggcc aggattcgcg ccttgccgcg cagatcgctg ctcgaagcgt
     2581 agaggccctc ggtgtaaccc atgagagtga tgagagcggc gtgtagcaca gcgatgccca
     2641 gcagcgaagc tggcgctccg gcggcgtact cgaacaatcg cacgcgcggg aacgcagagc
     2701 gtagcggcac cagcaatccg cccagcagaa gccagttgag gcccacgagc acaaagtcgg
     2761 ctgcaatgga gcgcagccag ctccaggcca tgcggccttg cagcggcact ggaaccagca
     2821 atgcgagcgc gccgtcgtgg ggatcggcgg gaagagccgg aatctccgat actttttcgc
     2881 cggcgacttc ccgagggcgc accgtgtgcg gcggcttgcg ggcaccacgc aaggcagaaa
     2941 cctcgcccgg aatcaaccgc gcgcagtcgg cagctgctgg tccttggcga gtggggtggg
     3001 aggcagggga agacacttct gggcagtccg tttcagatca tttccacgtc gtatccctgg
     3061 atctcgattg ccagcgagcg ctggatcgat tccatcgaga tgaccagctt gtctttctcg
     3121 cgctgaagca gcaggccttc gacgccgtcc aggcatccgc cgcagattct cactcgccgc
     3181 cccaattgca caaacgggta gaaggagaag ggcacttctt cggcgagaag gcgctgcaag
     3241 tcttcaatct gcttttgcgg aaccacagcc gcggctccgg cgaagctcac aaagcccatc
     3301 acgcccggag tttgcaagat caggaggcgc gcggccagtg agcgggcgga gtgtacaaag
     3361 gcatatcctg gaaacagcgg gaccatcacc accttgttgc gatcgctcca ggcgtgattc
     3421 tccttacgca gtggcaggaa gacctgcaac cccttggtgg acagctttcc ggcaactttc
     3481 ttctcgaaac ggtaccgcgt ctgcacggca aaccaggcct ctggcaccgg ttgcggcggc
     3541 caactgagcg cggcgctcac tgcttcctcc gtctccccga agcacagctc cgctccgtag
     3601 cttccatctg ggttttagtc atcttttcca cagacctcca actgccggct ttctctctac
     3661 agccgccggt aaatcgcctc ggggataggg aacgacctgt cgctcaaaac cgttcccagc
     3721 aaccgcgcct gcgcagcttc cagcacctct ttggctctga gggcggtggc gcggcgcgtg
     3781 tgaacggcag agagcacgag gatgatgccg tcggcgaatt gcgccatggc gatggtttca
     3841 ttcgactcgc cgacggggcg tgcctccaca atcgagtact caaactcccg gcgaatctcg
     3901 cccaggtacg aatgcagcga agcagccgtg accggatcgc cattgcgttc caaggcgggc
     3961 gcgagccaca ggttgccgcg caccgcggta gcgatgcggc gcagcggcat gttggcgcag
     4021 cgggccgcat ctgcggtttg ctcgggtcgg ttttgtgcat ccggcataga gtgcagagag
     4081 ccgccgatca cggctacact gcccgaagtc tcaagcgcga gggtctcgcc gatttgccgg
     4141 cagagatgcc gcaccacggt ctccggttcc accgcgctga ggacgacctg gcgaacaggc
     4201 cgcgctgcgc tggaaaaaaa gacttgccgg accaggccga gaatctgttc gcgggcaaag
     4261 ttttccggag tccaccgttc cggctgcttc aacttcttta tctcccgacg cggcctgtcc
     4321 cgatgcggaa cctgactcga atcgtgagcc gaatccgacg acgggaacag cactgttgct
     4381 gacgttccaa acttcatgcc aggaggctcc ttgacggatt tcgcggaaac gacgccagga
     4441 ccggtgcgtt caggtacagc aatacgtcat cgggggtacg gaacgcagga tcgacgtagt
     4501 caaccgcgaa cgctgctccc gtgccggctg cgccggcggc cgccagcccc acgcccagaa
     4561 ccgcgaacgt cgagaacaac gggagtgcag gaaccaccgg tggttccgcg atcgctacgt
     4621 tgacgatgcg ctgctgatcg agcgcgtcgg ccatgcgtgc ctcttcctgc ttcttcacat
     4681 aaagcagata gttctcctcg gcagctttct ccgtgctctg cagatctgcc tgcgtgatcg
     4741 catcgcgacc caggcggcgc gccaccgaat ggtaggcaga ctcctgcacg gccgctgcgg
     4801 cggcccgggc ctgcaaccct ttcaactcca cctggctgcg ctgcagttcg gccttgaccc
     4861 actcatagtg cgagtttcgg tcggtggtct cgtcgcgcac gggattcagg ttttccgcgg
     4921 caatggcagt tcgcacctgc gtgatttctt cttccacctc ctgcaccagg cggtggccgg
     4981 gttcaaactt ggtgagcagt tggatgcgct gctcctgcag ggcctgcaga cgggtcttca
     5041 acgcggtcag gactcccgga ttgtcggcga tgcgcacctg agtggtcgtg cgttcgggta
     5101 gcttcgccag caaagcgtcg agttccgaca ccttttgctg cgcctcagcc atttcgaccc
     5161 gggtttggtg atagagcgca tcctcatcgc tgagcctcag cagggcgcgg tcgcgttcct
     5221 cggcggcctc gaccacatcg cggccggtca tgaagtcgac caggctgcgc tgggcctctt
     5281 ccagttgccg acgggattct ctggtctgct gctcaaagaa gagaagctcg ccgccggggc
     5341 ggtgcactcc ggcatgtttt tccacgtagg cgccggctag agactgcaag acactggccg
     5401 cacggctggg atccgccgag gagtagctga ccgtgatcag gttggttttc tttacggggt
     5461 ctacatcgat ctgtttcgcc aggcggcggg ctgcgcgctc tacgcgttgc gcccggcctt
     5521 ccccgaggcg caggaagtgg aaccagtcgc gcccgcccag tgcgttctct tccacgactt
     5581 tgcgcaggat gtcttcatcc cggagcattt cgacttctga gttcaattcc tcttcgctaa
     5641 cggtgattcg ggtcagatcg agcggagcgt tttcctgggc gctcgcggga gcgtccgccc
     5701 ggcccggccg caccaacagc ttcatgtgag cctgatatcg ggctccggcg aaggcataaa
     5761 aaagcgcggc cgcaaacacc atcgctccaa cgcagacgaa aacccttcgc cgacggaaca
     5821 gcaccatgac caattcccgt acggttgggg ttggggctgg tggctgaggt cgccggcgaa
     5881 ttgggccttc gtctggcatg cgagcgggga aaactctcgt ttagaactgg gcggggtttg
     5941 tgtacatgct tacggaaggc ctggtcagga agcgagatat cttcgacaaa gcattctgcg
     6001 gaacgaacac taaatccccc ggctgaagat gagcgtcctc tttgaggccg ctctgcttca
     6061 gcattttctt taaattcagc agcctggttt ctaccaggtc gtcattgaca cgccggaaga
     6121 ggatcacctg cgagtgcttg gagtacgagg taaagcctcc ggcaatctcc acggcctcag
     6181 cgaccgtggt gtcggtgcgt aattggtact tgccgggccg ggcaatttcg cccccaacga
     6241 catagtaagg acgggcgaag tccctcaaag ccacggctac ttgcgggtcg tgcaggtatc
     6301 cctgataggc ccgttgcacg gctgcggcga attcctcgag attcagtccc tgcaccgcga
     6361 ccatgcccgc atccttgagc gtgacgtagc cgtcgggctg gacggtcaga gtctggttga
     6421 actcgggcgc aaccgtaaaa ctgatttcga ctacatcgct gggcgctaac cggtagagcg
     6481 gacggcgttc ccctccgagc aaagggtttc ccaggccgtc cgctttcccg ggttttgcgg
     6541 ccgcagtact gtcgcttccc tgttctgccc cggtaaagct ggacggggaa gcctgcttgc
     6601 catcgctcag gttctttctc ccttcctcct gcgcgaatgc ggccgaagca cagttcaaca
     6661 acagaagcgc gctggctatg aaacgggcga atataggaac gcgcttcact tcttcctgca
     6721 ccacactcag attctcaacg ccactgcttc tcgtgagttc tgctcgcaaa gtaagccagg
     6781 ccgtcctcta caacaagaca tggaaagtgg cgtctacaaa agtggatggg tgtctgcgct
     6841 gcggagatga gcagccgcac gaaaggacgt atcggaatca agaggttgcg aggaactcct
     6901 ctagccgcag aatgctagac acgtcacggc aatgcctctc gatggagtat ccacaaaagt
     6961 ggacttgcag tgtttgccat gtcacagggt cggttcgtca cggcctggcg ccgatggagt
     7021 cgttcttctc gcttcgtccg cgagcaataa gcgaaacttt atgtgcagcg cggcttttca
     7081 cgcatcccga gccgcaagcg tggatcttct ggccactttc ttcatctgca ttcgcgcccg
     7141 attctgacca cgctttgcac agatcgatct tccgtgtgca ggacgctcgc agtaccggat
     7201 tgagaattcc cattttgaaa gcagtgccca ttttttgtga gcagtttcct tgtgggctgc
     7261 aggctcagtt cctaaaatcc cactattcgt cgttgttcgt cccggagttc ccaagggacc
     7321 aggcttatga gcaaattgcg cgcagccact tctaacggtc ttggcgtact ggtagcagac
     7381 tcgaaccgca tgcaggccca gttgctcact cgcgcgctcc ggcgccgtgc cgagtttcgc
     7441 atcgccacct gccggatgga cacgatctct attctcgagg cggttgcagc gcgtgtgccg
     7501 caggtggcgt tgctggcttt gagttcgccc accagcctta ccgaaacgat catggccctg
     7561 cggcgatttc atttgtcgca tcccgaaatc cccaaggttc tgctggtgga ctctgacgac
     7621 cgcgatgtgg ttgtcagcgc atttcgctcc ggcgcccggg gaatcctgtc cattactgaa
     7681 gccaacctgc gcctgctctg caaatgcatc ctccgcgtgg ccgaaggcca gatctgggcc
     7741 aataccgcgc aactgaagtg cctcatcgac ctgatctcgg aagttccctc gctgcgcgtt
     7801 ctcaactccc gcggcgattc gctgctgact ccgcgcgaag aacaggtggt tgcgctggtg
     7861 gccgaaggcc tgggcaaccg gcagatcgcg cacgagctca acctcagcga gcacacgatc
     7921 aagaagtatc tattccgcat cttcgagaag ctcggaattt ccactcgggt cgaactcgtc
     7981 ctctacgcag tcaatcatgg acaaccgcgg caggcggaat ggctggccgg cacgagaagc
     8041 gcctgaatgc aggcaagcca gagtcggatg ttcccagtaa cgtgtgagct tccgaggcct
     8101 cggctcctat ttgaaaacct tcaccctgcc ctgtataact tgggacgaaa gccgtgcccg
     8161 aggccgattc caatcccgtt cctgcgccca agacgcggcg ctggaccatc gtcgccgccg
     8221 ggctgctcct gctggccctc tccctggtcg tcatttacac gagccaccgc gcgttcatca
     8281 gccccctggc gctcgtagtc gtggcagcga ttggactcgc tgcccttctg ctccagctgc
     8341 ggctgcgaaa ggacatcagg gttccggtgc gggcgccgct gctactcaac tggttgggac
     8401 tcttgttcgc gatggcggcc gttgtagccg acttctttca tttcggcggc ggggtgatga
     8461 tagtggtggc gctgggagct gtgctgtgct tcgccatcag cggaatcttt gtgctgcacg
     8521 cgttgcgaaa gcagtgaagt ccggcttccg ctttctacct tccggcgctc aggaaagagc
     8581 agaaaactgc caggggccga tctcgcgggc tttccggaag ccgagagccg ggagctggcc
     8641 tagcgcttga attcgaccgc cacctgggtt tcgacaacgg cgggctcgcc gttcaggcga
     8701 tagggttcga acttccacca gcgcagggca tccatcgcgg cgcgtgccaa tatctcaggg
     8761 ccgttgaggg cacgcgtact aattaccgat ccatcgcgtc ccaccacgat atccaacgca
     8821 ataattccct gcagattggc tcggcgcgcc tcggcgggat acacgggctc gacccggtgg
     8881 atgatcagct tctccatgac ttccgcggga acctgggctt ctggcttctc ggcagcggtg
     8941 gtctcctcac tcttcagtcc ggattccagt tcttcccagg cccgattcca cttccaccag
     9001 aaaacggcgg cagcaaccgc gagaaagaaa agaaagacaa tccacaccgt tccacgccgt
     9061 ttttttcctc gcgatcgatt gttgcgtccg ctcccgttcg cggatcgagt ccggtcggct
     9121 ttttgtccct cttcgtcacg gaaatcgcgg ctctcggcca ccgggagtgc gctgatttcg
     9181 ggctcggcct tggattcctt cacccagtca cgaatcgctg cccgctgctc agctgacaag
     9241 ccaacgaact caagcccaca gcgcaggttc tcctgatacc gcacggtcgc tcgcgtacgc
     9301 agcggatcgg atagaagcgg cagctggact tcaagaccga cggcttcgcc cggcgtcagt
     9361 tcgcccgcca gcatggcggc aatgccgccc tcacacacgt tcactgaacg cccaggcacg
     9421 gtctcgggaa tgcccgaccg gagcacggtt acgtccaggg gcgagtgaag acggtagcgc
     9481 ggcacgcgtc tccgcgaagg cccgttccat gccggaatgt gttcgcctgc tgccattgga
     9541 tgcctgccat ctatcgccgc agttccacaa tctcgtttcc ccgggcaagg agcacgacgc
     9601 tcgccattcc gatgaacaac ccgtgctcaa ccacgcccgg catgtcgctg agctggcgag
     9661 ccagcgcgtc ggcatctcga atctcgccaa agtggcaatc gagaatgtgg ttgttctcgt
     9721 cggtcaggaa cggcttgcca tcctccccgg ctcgcacttg cacctcggcc ccaagggctc
     9781 ggattcgctt cgccaccagc ggctgcgcga acttgatgac ctcgaccggg agcgggaact
     9841 tccccagcaa aggcacctgt ttcgaggcat cggccacgat caccagctgg cgcgtcgctg
     9901 aggcaacgat tttttcccgc aatagcgccc cgccgcctcc cttgatcaga cgcaggtgtg
     9961 gatcgacttc atcggccccg tcgaccgtca cggcaatgtc ctggcactca tcgagcgacg
    10021 tcagcgggat tcccaggctc atcgccaggt cgcgcgaacg caccgaggtt gggatgccgc
    10081 ggatgcgcag accgttcttc acctgctcgc ccagcagttt gatgaagtag gtcgctgttg
    10141 atccggagcc cagcccgact acctgtccgt ctttaataaa gcgcaggctg gccctagccg
    10201 ccgtttcttt ttcctgatcg ttcgccatag atcgtaagta tcgtagcgcc gctcatctca
    10261 gactcgtttc ggtatagatc agataggccg ccaggaccag gccgaacagc gccgtaagaa
    10321 ctgtaaccag gctcagcaga aatcccgccg gctcaaaggc tccttcatcg agctgcactc
    10381 gcgtcttgcg atagcgcgcc agccccgcga ccacgagcgc cacacccagg aggatcgaga
    10441 acgcgccgat ccacacggaa agtccagcgg ttcgcagagg gtggccgctg atttcggtca
    10501 gctggcgcat ggcgatcgca aaacgtccga tggcgaagcc gaacacaacg atggccgcgc
    10561 ccgtccgcac ccaggcaagg aacgtgcgct cgttggccag gtgatcccgg gcgcgattgg
    10621 ggttggcgtt gtccacagag atcctccgaa gacgccgccc attattccac agccacggtc
    10681 agcgggcgcg gggtggccat gcgctcgcgg tagatcaggt ttttcgcttc cacttcgccg
    10741 atggcgtcga cgtgcaccac gtatcctccg cgcggcaagg tctcgccaaa gggcagctct
    10801 ttcttggtgg tgccggcggg cagcacgaaa tgctggtatc cgatggcggt cgcccgcccg
    10861 ttttcgggaa ctgccagcac gatgaaggtc agatcgatat tgtcgggcgt gctattggta
    10921 acttcggcgc tgccttcaat ccggttcccg tttacgcgcg ccggggactt ccagttgagg
    10981 acgagcgcgg agtgcacgtg gatcacaaac aacttcttca cggtcacctg gggctggccg
    11041 ctgtcggtca ccacggctgt aaattgaaaa tcgcccaccc ggccaatttg tccatggagc
    11101 aggccgttgt cctgaagggt gattcccggc ggcaatgcgc cattctcggc ccgccaatgc
    11161 agcgggggca cgccgccccg ggcgcggaaa taaacttcgt atctcgcgcc aggataggtt
    11221 tcggctagcg gggacgcgtt ctcgatcatc aaggtctgac tggactgcgc tgtcggcgca
    11281 ccgctctgcg ctgcggccgg tggtaccagg caacacactg gccaaacgcc gcaggcaagc
    11341 accagcagcc tgctcattgt ttcccgcatg caacgcaaat tcctcatgcc agccatccag
    11401 ggactaccgt ttcccgctgc gcactgcagc cctgtggtta tcatcacata gaaagagcgg
    11461 cggggcgaaa cttgcagtcg tgccctcagt ttgaaatctc agtatgtacc ggcgtcatct
    11521 cgaagtcccg cgccttcacc ggcgggacaa gagacctccc acgcaacggc tttatacatc
    11581 gggagatcac tagctccacc tcgctgcgct cgcctccagc tgaaagccgg ggggtctacc
    11641 aaaaacggtt tcctggggat gacgcccggt cgcagttcgg caaccgcgtc acggaagtca
    11701 cattgactca gattcgatcc acaagccttc acaaccggcg gagcaacgaa atcctatggg
    11761 agcagatccc catcccactt ttccaggctt gagggaatcg accctttgag cagggcggca
    11821 aaagtctcga gcgagttgcg ggcatctccg atcgggtagt agaacgcttc ccagttgtcg
    11881 ctgtcctcgc cgtcgtgatt cgcgatgacg tattggaaac gccacgcctc tttcatctcg
    11941 cgataggcgc tgcccgcgcg agtttcgata tcctccagat ctttggccga gagctcttgc
    12001 ttctggcggc ttgtgcggcg cagcagcttg cgtcgcatca cgtccgtgac gaagtccgga
    12061 agcgacaggt tccgctccgg agccttcaga tagacaatct cttcccttga gagcggcgat
    12121 acaaaaacac tgagccggtt tacggcagtg aggcgagggt gcgtttggag cacccgaccg
    12181 acgaagggac tgccctcgaa gaggacgtcg ccgctttcca gcaaagagtt gagttcctgg
    12241 gcgtccagcg cctgcaggtc actgcgcacc tcaatcagca cataacgtcc gtccgactgc
    12301 aacgactcga tctgagtgcg cgtgcggaag tgatagtcca cgccatcgac ttcgccggga
    12361 cgcggacgcc ggctgttgaa cagcaccaac ttgcgcagct gggccgacag ctcgggatag
    12421 aacttcgcca gggatttatg gagcggagtt ttgccgacgc aggagggccc gctgagaatt
    12481 acgagacgac cggggcgcgg agaattcgtc atcgccagat ggcaggagga taacgcggta
    12541 cggggaaaac ggcgggaaaa aaataagagt tccgaggtgg tgtgggatcc attcctctcg
    12601 gccagtagct cgcttctgac cctcgctcga cctgctctca gctctgccct tgcgaggctg
    12661 cgctgcgcga tctctgcttg ggctccgggc tcaccgccaa ccggataaat ctaccccacg
    12721 cctcggaact ctatcttgcc ttcaacaggt ctcccctgtt ctcctcgacc agcgctttgc
    12781 tatagaccct cgcctgccct cttccccagc tttgcagcca ggaaagtgac atcggcttga
    12841 gccccggacg ccgcaccaac cggaaaaact caagaagctg tcaaagaacg attatgtttc
    12901 tactccttct attttttccg tcaacgaaaa atcgcggctt gtaaccagtc gtgttttcag
    12961 tcgagtgcag accgtccaca aacccatgac catatttagt gcaaacggga gctcgatttc
    13021 tgctcgagaa ccatcttctg gtgcggtttc ccgcaattcc gtggcagcgg ggaagaagcg
    13081 tcaagtcagc ggagaatttg cgcggtcctg tgaaaatcgc gtgcttctcg ctctgggcga
    13141 gggtttgaga gatctgcctg cctaggcctg tttgcttctc ttgccgccga gcgtgccttt
    13201 tgcagttgcg cccttgcccc ttcccatccc ggcaatgtcg gctacggtcg tgttctcgag
    13261 gatcttcgcg gcggcgtcgc gcacgtcgag aaataccttg tagagggcgc gatgatcggc
    13321 gtctttggcg attaagccgc gcagctgttc ggcatctccc agaggcgcca gagggccatc
    13381 gatgagccgg atgatgtcgc tcaggggaat ctcggcggga tcgcggcgca ggcagtagcc
    13441 gccgcgggcg ccgcgcacgc tgtcgacgat gcgggcgttc ttcagctcca gcaggatcag
    13501 ctccagaaac ttttcgggca ggtcgctatc ggcagcgata tcgcgaatct tgattgcgcc
    13561 ctgatcatac cgccgcgcca gagtcatcat ggcctgcagg gcgtagagtc ccttctgcga
    13621 aatcttcaat cagcgtctcc cgacccgcct aattgtgcct gaattcttct tctctgcata
    13681 gacaaagtgc ggtacggcag tggccagtgg gcagtggtcg gggggcagcg gtcttaggca
    13741 gtgggcagtt gtcagttgtc agcaacacgg tgtctgaaat cggccggcgc gcgatgattc
    13801 cggagactgg caactgatca ctgatcactg attttctggt gccatcgaag ggactcgaac
    13861 ccccacaccg ttgccggtac atggacctga accatgcgcg tctgccaatt ccgccacgat
    13921 ggcaagtgga cttcaaagtc gcggcggcca ctg
//
LOCUS       DASPUL010000287         4895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_48924, whole genome shotgun sequence.
ACCESSION   DASPUL010000287 DASPUL010000000
VERSION     DASPUL010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48924"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..865)
                     /locus_tag="VEJ00_07050"
     CDS             complement(293..865)
                     /locus_tag="VEJ00_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00953.1"
                     /translation="MRSLKHHQTMVLTLLLAAGAVALAQRHRDPLTQPEIDQIRDTSW
                     EPRERLKLYVEFARARLVKLEQMRSDPKTKDRATQTHDLLDDFQLLYDELNDNIDTYV
                     DRKDDIRKPLKLIIEADTEFQAKLRALKDAADVPAAETSTYEFVLTNALDTVDTSAEE
                     HRKLLADQEDAAKHKKLNSSSNTKTAGKPE"
     gene            complement(862..1320)
                     /locus_tag="VEJ00_07055"
     CDS             complement(862..1320)
                     /locus_tag="VEJ00_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00954.1"
                     /translation="MPGWKLAIRLGVALALCALLVAAPARAGELSVDELKAKLGAVSP
                     GDKPHICVQIAQQQLAAADQLYAAVEVEKAQAALTDVVAFSELARDYAIQSHKYQKQT
                     EIAVRAMTRKLNDIMHGLPHDDRPPVHDAIERLQRVRDDLLAAMFPKGTK"
     gene            complement(1342..2685)
                     /locus_tag="VEJ00_07060"
     CDS             complement(1342..2685)
                     /locus_tag="VEJ00_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX00955.1"
                     /translation="MSAGAQVSQNASPGAAAQSSDSSGNQQSQTDDRGWSSSKKGRHV
                     RVPEEAPAAPELSQAEDLIQKKDYSAAEALLSKAVSVDAANYVAWFDLGFVDNALGKV
                     DDSITAYRKSVAAKPDVFESNLNLGLQLAKTGQPDAEQYLRAATQLKPTSHVAEGLER
                     AWLSLGHVIEKTSLDDAVVAYQKAAILQPKDPEPHLAAGQLLEGQNKFSDAEHEYQQA
                     LALDPSSDALTALANISMRGRRFPEAEDYLRKLAAAHPGDAEPHVQLGRVLAAQDKND
                     DAIAELQAGLKLAPADLSVQRDLADLYATTGKNDLAEAAYRGLIAANPNDPRLHHELG
                     KSLLLQKKFPEAQQELLTTVKIKPDFGEAYGDLAFAASENKDYVLTVKSLDARAKFLP
                     EIPITYFLRASAYDHLRDAKQAAANYHLFLESAKGKYPDQEWQAKHRLIAIEPKK"
     gene            complement(2726..>4895)
                     /gene="uvrA"
                     /locus_tag="VEJ00_07065"
     CDS             complement(2726..>4895)
                     /gene="uvrA"
                     /locus_tag="VEJ00_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXX00956.1"
                     /translation="ASGGIEEVAGKSARPTLRFAQRFECKHCNQRYEEPEPRLFSFNN
                     PYGACPRCQGFGNTIDFDLDLVIPDKMKTLGEGAIEPWTKPKYRPLFTDLKRYARQAD
                     IPLDVPWADLAEEQRNLVLNGEGRFLGVRGFFQHLERKKYKLHVRVFLSRYRGYSLCP
                     DCNGSRLRLEARQVKINGKNICELCALTVEDAFRFFEKLELSAEQAEIADKLLQEIRE
                     RLRFLNDVGLEYLTLDRLSSTLSGGEAQRIQLATSLGSRLVGTLYVLDEPSIGLHARD
                     THRLIKILHDLRNLGNTILVVEHDPDIMRAADRILDLGPGAGENGGKLVAVGTYDEIV
                     RNPTSLTGRYLAEDLRIQAPITRRKPGPQQIRLRGVRANNIKGLDISVPLGMIVAITG
                     VSGSGKSTLLHQVLYRALAQAKGQSSNGATGPSASWETLEGEQFIEEVVLVDQSPIGR
                     TPRSNPVTYIKAFDAIRDLFASLPEAQKRGFGTGHFSFNIPGGRCETCQGDGTVTVEM
                     QFLADVELICEECKGTRYKPQVLEVRYRGKNIHEVLNLTVKEALKFFAEAPRVTEKLR
                     TLEEVGLGYLRLGQSATTLSGGEAQRMKLAAHLQPASRESARSARRSGDARSRQRLLY
                     IFDEPTTGLHFDDVSKLLAAFRRLIEAGGSIVVIEHNLEVIKTADWVIDLGPEGGNRG
                     GKVVGVGPPEAIAKLPGSYTGKYLARILSGNGSSRSNGRS"
BASE COUNT          866 a         1476 c         1503 g         1050 t
ORIGIN      
        1 agtggtcagt ggccagtggc ttcgtaaaag cgaggcatca caatctacaa ctgaaatcgg
       61 tgtcgtccca cgggactcgc ttcatttccc gcttgacccg gggcttacgc cccgggctat
      121 cacatgccgc cgcttcgcgg ctgggattgt gcggtttcgg cccgccggtt cgcctccaaa
      181 ttccggtcgc acgcgcactc gcaattcgta tgccggggga ggcaggccgg aagccgctcc
      241 catccaaccg ctttaaagac taggttcgga attgctgacc actggccact gatcactccg
      301 gttttccggc ggttttggtg ttcgaactcg agttcagctt cttgtgcttg gccgcgtctt
      361 cctggtccgc cagcagtttg cgatgctcct cggctgatgt gtctacggta tcgagggcat
      421 tggtcagcac gaattcgtag gtgctcgttt ccgccgcggg cacgtccgcg gcatccttta
      481 gcgcccgcag tttcgcctgg aactcggtgt cggcctcgat gatcagctta agcggcttgc
      541 ggatatcgtc cttgcggtcg acgtaggtgt cgatgttgtc gttgagttca tcatagagga
      601 gctggaaatc gtcgagcagg tcgtgcgtct gggtggcgcg atccttggtc ttgggatcgc
      661 tgcgcatctg ctcgagcttc accagccgtg cgcgggcaaa ttcgacatag agcttcaatc
      721 gctcgcgcgg ctcccagctg gtgtctcgaa tctggtcgat ctccggttgc gtaagcgggt
      781 cgcggtggcg ctgtgcgagg gcaacagcgc cggccgccaa cagcagcgtc agaaccattg
      841 tctggtggtg cttgagcgat ctcatttggt gcctttcgga aacatggcgg ccagcagatc
      901 gtcgcgcacg cgctgcaacc gttcgatagc gtcgtgcacc ggggggcggt cgtcatgcgg
      961 cagtccgtgc ataatgtcat tcaactttcg ggtcatggcg cgtaccgcga tttcggtctg
     1021 cttctggtac ttgtgcgatt gaatggcata gtcgcgcgcg agttcggaga aggcgacgac
     1081 gtcggtaagc gcagcctgcg ccttctcaac ctcgacggca gcgtagagct ggtcggccgc
     1141 ggccagttgc tgctgggcaa tctggacgca gatgtgcggc ttgtcccccg ggctgaccgc
     1201 ccccagcttc gcttttagtt cgtcgacgct gagctcgccg gcgcgggcag gggccgcaac
     1261 caacagagcg cagagcgcaa gcgccacgcc caatcgaatt gcgagcttcc agccgggcat
     1321 gatgcaaaat ccaacgtgca gttacttctt aggctcgatg gcaatcaatc ggtgcttcgc
     1381 ttgccattcc tgatcaggat acttcccttt ggcgctctca aggaagaggt gatagttcgc
     1441 cgctgcctgt ttcgcatccc tgaggtggtc gtaggccgag gctcgcagaa agtaggtgat
     1501 gggaatctcc ggcagaaact tcgcgcgggc gtcgagagac ttgacggtta agacatagtc
     1561 cttgttttcg ctggcagcaa aggccaggtc gccataggct tcgccaaagt ccggcttgat
     1621 cttcactgtt gtgagcaact cctgctgggc ttccggaaat ttcttctgca acagcaggct
     1681 cttccccagt tcgtggtgca atcggggatc atttgggttc gctgcaatca gccctctata
     1741 ggccgcttcc gcgagatcgt tctttcccgt cgtcgcgtaa agatcagcca ggtcgcgttg
     1801 cacggaaagg tccgcgggcg ccagtttcaa cccggcttgc agttcggcga tcgcgtcgtc
     1861 gttcttgtcc tgagccgcga gaacccttcc cagttgcacg tggggctctg cgtcaccggg
     1921 atgggcagcc gccagtttgc gcaagtaatc ctcggcttcc ggaaagcgcc gcccgcgcat
     1981 ggagatattg gccagcgccg tgagtgcgtc cgaagaggga tcgagcgcca gagcttgctg
     2041 atattcgtgc tcggcatcgg aaaacttgtt ttgaccttcc agcagttggc cagcggccag
     2101 atgcggctca gggtctttgg gttgcaggat ggccgccttc tgataggcga cgacggcatc
     2161 gtcaagcgac gttttctcga tcacgtgccc cagcgagagc caggcacgtt ccaggccttc
     2221 ggcgacgtgg ctggtcggtt tgagctgcgt cgccgcgcgc aggtattgtt ccgcatccgg
     2281 ttgtcccgtc ttagccagct gcagcccaag attcaggttt gactcgaaaa catccggctt
     2341 ggccgcgacc gacttccgat acgcggtaat ggaatcgtca accttgccca atgcattgtc
     2401 gacaaagccc aggtcaaacc aggcgacata gtttgcggcg tcaacggaga ccgctttgct
     2461 aagcagagcc tcagcggcgg agtagtcttt cttctggatg aggtcttccg cctgcgagag
     2521 ctctggcgct gccggcgctt cttccggaac cctgacgtgg cgccccttct tgctgctgct
     2581 ccatccgcgg tcgtcggtct gcgattgctg gttgccagag gagtctgacg actgagcggc
     2641 cgcaccagga gacgcattct gactgacctg ggcgcctgca gacacacatg cggccaccca
     2701 tgccgcgcag gcggcaagag cgaccctacg agcgaccatt ggaccgcgag ctgccattac
     2761 cactcaaaat gcgtgccaga tacttcccag tgtaggaccc gggcaatttc gcgatggctt
     2821 ccggcggccc gactccaacg accttgcccc cacgattccc gccttcgggg ccgagatcga
     2881 tgacccagtc cgcagtcttg atgacctcaa gattgtgctc aatgacgaca atcgaccccc
     2941 ctgcttcgat aagacggcgg aaggcagcca ggagtttgct gacgtcgtcg aaatgcaggc
     3001 cggtggtggg ctcgtcgaag atgtatagca gccgctggcg tgaccgcgcg tcgccagaac
     3061 ggcgcgccga cctggcggat tcgcgggaag ccggttgcag gtgcgccgcc agcttcatgc
     3121 gctgggcctc tccgcctgag agcgttgtcg ccgattggcc gagccgcaag tatcccaagc
     3181 ctacttcctc gagggtgcgc agcttctcgg tcacgcgcgg ggcttcggca aagaacttga
     3241 gcgcctcttt caccgtcagg ttcagcactt cgtgaatatt cttgccgcgg tagcggactt
     3301 cgagcacctg cggcttgtag cgtgtgcctt tgcactcttc gcagatcaat tcgacgtcgg
     3361 ccaggaactg catctcgacc gtaaccgtgc cgtcgccctg gcaagtttcg cagcgtccgc
     3421 cggggatgtt aaaggagaaa tgtcccgttc cgaagcctcg tttttgcgcc tcgggcaggg
     3481 aagcgaacaa gtcgcggatc gcgtcgaatg ctttgatata agtcaccgga ttcgagcgcg
     3541 gcgtgcgccc gatgggggac tgatcgacca gcacaacctc ctcgatgaat tgctcaccct
     3601 ccagggtctc ccagcttgca gacggtccgg tcgctccgtt gctcgactgt cctttggctt
     3661 gagcgagtgc gcggtagagc acctgatgca gaagcgtcga tttacccgag ccggagactc
     3721 cggtaatggc cacaatcatg cccaggggaa cgctgatgtc gagccccttg atattgttcg
     3781 cgcgaacacc gcgaagtcgg atctgctgtg ggccgggctt gcggcgcgtg atcggtgcct
     3841 gaatccgaag gtcctcggcc agatatcttc ccgtcaaaga ggtcgggtta cgcacgattt
     3901 cgtcataggt gccgactgcg acgagcttgc cgccattctc gcccgcaccc gggccgagat
     3961 cgagaatgcg gtctgctgct cgcatgatgt cgggatcgtg ctcgacgaca aggatcgtgt
     4021 tcccgagatt gcgcaggtca tgcagaatct tgatcagccg atgcgtgtcg cgcgcgtgga
     4081 ggccgatgga gggctcatcc agaacataga gcgtgcccac cagccgcgag cccagcgaag
     4141 tggcgagctg gatgcgctgc gcctcgcccc cggagagcgt cgaggacagc cggtccagcg
     4201 tcagatattc caggcccacg tcattcagga agcgcagccg ctcgcgaatc tcctgcagca
     4261 gcttgtcggc gatttctgct tgctcggcgc tcaactccag tttctcgaag aaacggaagg
     4321 cgtcttctac ggtgagagca cacagctcgc aaatgttctt cccgttgatc ttcacctgcc
     4381 gggcctcgag ccgcagacgc gacccgttgc aatccgggca cagcgaatac ccgcgatagc
     4441 ggctgagaaa cacgcgcacg tgcagcttgt atttcttgcg ctccaggtgc tgaaagaatc
     4501 cacgaacgcc caggaagcgc ccttcaccgt tcagaaccag attccgctgc tcctctgcca
     4561 ggtccgccca gggcacgtcg agtggaatgt cggcctgccg ggcgtagcgc ttgagatcgg
     4621 taaacagggg gcggtacttg ggcttggtcc agggctcgat cgcaccctca cccagggttt
     4681 tcatcttgtc gggaatcacg aggtcgagat cgaagtcaat cgtgttgccg aatccctggc
     4741 agcgcgggca cgctccgtat ggattgttga aggagaaaag tcgcggctcc ggttcctcgt
     4801 aacgctgatt gcagtgcttg cactcgaaac gctgcgcaaa gcgtaatgtg gggcgggcac
     4861 tcttgcccgc cacttcttca atgccgcctg acgcg
//
LOCUS       DASPUL010000288         5147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1552792, whole genome shotgun sequence.
ACCESSION   DASPUL010000288 DASPUL010000000
VERSION     DASPUL010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1552792"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VEJ00_07070"
     CDS             <1..462
                     /locus_tag="VEJ00_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX00957.1"
                     /translation="DFVNTTREGFRVAHDVSVYSLIAVSRAAAPLMEDGGSILTMTYY
                     GAEKVVPHYNVMAVAKAALECTVRYLAHDLGKKKIRVNAISAGPIKTLAARGISGLGD
                     MLKSHAERAPLGRNVEVAEVGSTGVFLASDASSGITGEVIYVDCGYNIMGF"
     gene            654..1592
                     /locus_tag="VEJ00_07075"
     CDS             654..1592
                     /locus_tag="VEJ00_07075"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXX00958.1"
                     /translation="MKRKRIFIAAALILVPLALFLHTFSASPLQKPEPYAGPIPSATP
                     PDGMAVYALVAGVNHRVAAFGYRGGSFFDRRDFAMAGALVEHPKGDLLIDTGFGRHID
                     EQFRTLPFMLRAVTFYSLWRPAADQLTDAGYDLKSLHAILLTHSHWDHVSGLPDFPGV
                     PVWVTPQEHEFLQKSGYANFCKLFTGIRYEEYGFEGGSYLGYPASHDVYGDGSIVVVP
                     AFGHTPGSVIIFVTLPNRTRYAFVGDLVWQLEGITEREERPWSTRRRADTDAEANREN
                     LLRMIALKERMPDLIIVPAHDIRAFQTLPRLPQKQK"
     gene            2086..4047
                     /locus_tag="VEJ00_07080"
     CDS             2086..4047
                     /locus_tag="VEJ00_07080"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXX00959.1"
                     /translation="MTPGTLSRVIALLVLSPLALSQAAPSRASAAVDSLPKVKKITQV
                     AISPDGTQVAYIVDGHLTVVSLADNATHAIEVEGKLPLRSVAWKSDSKTVAFVADLPG
                     NAPSAQIWTASSDGSSPSKRAELKGNVDASSFSPDGSRLAILFIEDMPRKAGPLEPMT
                     PLAGVVGSKVYEQRIATIDLAANQLTQITPADMYVYEYDWTPDGRAWVAAAAHGDGDA
                     NWYVARLYQFDAHSGEPREIYKPKLQIASPQVSPDGKNVAFIEGLMSDEGPTGGDVFV
                     VPIAGGSARNLTPNRNASPSAVYWRDASHIVFAEIADGHSAFAKVSTAGGTPQTLWSG
                     EEFIGTDSTPTASFSHDSAVSAVVRESANTAPEVWAGVLGHWKQISTANADAKPAWGE
                     TRNFHWNNGKTRVQGMLTLPNDYDAAKTYPLVVSVHGGPSSACQPRWNEREIGAMSAM
                     GYFVLCPNPRGSYGRGETFTQANVKDFGGGDFRDIMAGIDAVSKDFPIDPQRIGIRGH
                     SYGGYMTMWAETQTHRFAAAVAGAGLSDWLSYYGVNEIDEWMIPFFGASIYDDPAVYA
                     KSDPMHFVKSVQTPTLILVGDSDGEVPMEQSVEWWHALNTMKVPTALVVYPHEGHIFG
                     NPDDSRDYTLRTLEWFEEWFAKAASSAKR"
     gene            complement(4044..4511)
                     /gene="tsaE"
                     /locus_tag="VEJ00_07085"
     CDS             complement(4044..4511)
                     /gene="tsaE"
                     /locus_tag="VEJ00_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXX00960.1"
                     /translation="MSDQATAKTVLREITTRSAEETIAFGRTLAELLAPPKLVLLRGE
                     LGAGKTTLVKGIAAAFEAAAEEDVTSPTFTLVHEYRGPRASLYHIDLYRVDTPRELET
                     LALDDLRSDDGILLIEWGEKFPRLLRECDVEISLEREGETRRKIRVVGSDNSV"
     gene            complement(4673..5059)
                     /locus_tag="VEJ00_07090"
     CDS             complement(4673..5059)
                     /locus_tag="VEJ00_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00961.1"
                     /translation="MTHFPQPHPSRRAARIQLGDSVLAAIRLEDGRRTKAKLQTISVT
                     GGLLRIAQSLGQGDFVEVAFQTQAGPVHGMAEVLSPMRKTTDGVLQPFRFVALEDDDH
                     RRLRTSLDHVAERSVLGLKSSAFSTH"
BASE COUNT         1052 a         1611 c         1504 g          980 t
ORIGIN      
        1 gacttcgtca acaccacgcg cgaaggcttc cgcgtcgcgc acgacgtgag cgtctattcg
       61 ctgatcgccg tttcgcgcgc cgccgcgccg ctcatggaag acggcggatc catcctcacg
      121 atgacctact atggcgccga aaaggtcgtc ccgcactaca acgtgatggc cgtggccaaa
      181 gccgcgctcg aatgcaccgt gcgctacctg gcgcacgatc tcggcaaaaa gaaaattcgc
      241 gtcaacgcca tctccgccgg cccgattaaa accctcgccg cccgcggcat ctccggcctg
      301 ggagacatgc tgaagtccca cgccgagcgc gccccgctcg gccgcaacgt ggaagtggca
      361 gaagtgggat cgacgggcgt tttcctcgcc tccgacgcct cgagcggaat taccggtgaa
      421 gtgatctacg tggattgcgg gtataacatc atggggtttt gaaaaccagt tctcagtact
      481 cagtactcag ttgtcagtag tcggtgggtg ccccacccgc agcctgccct gagcgaagtc
      541 gaagggcgct tttcgtaggg tggggatttt gactccctgt gcgccattgt ttttcactgc
      601 aggttacccc gaactgtgcc cctttttccc tcgtagcgag gaggaggcag gcaatgaagc
      661 gcaagagaat tttcatcgcg gcagcgctga ttcttgtgcc gctcgctctc ttcctgcaca
      721 cattttcagc gtcaccgctg cagaaaccag agccctacgc ggggccgata ccctcagcca
      781 caccgcccga cggaatggcc gtctacgccc tggtggctgg tgtgaaccac cgagttgcgg
      841 ccttcggata tcgcggcggc tcgttcttcg accggcgcga cttcgcgatg gccggcgctt
      901 tggtggaaca cccgaagggc gacctgctca tcgataccgg gtttggccgt catatcgacg
      961 agcagtttcg gacgctgcct ttcatgcttc gggctgtcac gttctatagt ctgtggcggc
     1021 cagcagccga ccaactaacg gatgctggct acgacctaaa atcgctgcac gccattcttc
     1081 tgactcattc tcactgggac cacgtcagcg gcctgcctga ttttcccggt gtgccggtgt
     1141 gggtaacgcc gcaggaacac gagttcctcc agaaaagcgg gtacgcgaat ttttgcaaac
     1201 tcttcaccgg catacgctat gaggaatatg ggttcgaagg gggctcttac cttgggtatc
     1261 ctgccagcca cgatgtttac ggcgacggct cgatcgtggt ggtgcctgca ttcggccaca
     1321 ctcccggcag cgtgatcatc ttcgtgacgc tgcctaatcg aacgcgctat gcgtttgtcg
     1381 gcgacctggt atggcagttg gagggaatca ccgagcgaga ggaacgtccc tggagcaccc
     1441 gcagaagggc cgatacggac gcggaagcaa accgcgaaaa cctgctgcgc atgatcgcgc
     1501 tcaaagaaag aatgcccgac ctgatcatcg tcccagcgca cgacatacgc gcttttcaaa
     1561 cattgccgag actgccgcaa aagcaaaaat agcgctcctg aagcaactcc tggaccgctt
     1621 ccgttttcca cagacttttt tcgcgcgtgt gacgaacgaa ctttaccgag aaacctggcc
     1681 tgcctaatct ggcagcgtag gtaccggatg tgatgccgcc ccagtcctag gaaactggcg
     1741 gccccagcag cctccccatg cgagcgagga acgggacatc cgatcagggt gctgcagaca
     1801 gctaccccga atggctcggg atctggcgcc cgttgtggga gtgatcactc ctaacgttgt
     1861 tgaaaacaat gaacgacgca agaaaaccaa agtgaaaaca accaggaacg gtagaacagg
     1921 acaaaacagc agacaagcga actagacaag cgagctaaag aacaccgatc agcgtacgac
     1981 gccgactcgt atcagggcac accttcaggt gttgaggaac cgccgcgccg acctccgccg
     2041 cgccgtcgcc gcatcccgcc catccagcct gtatactcct ctgccatgac tccaggcacg
     2101 ctttcccgtg tcatcgcatt gctcgtgttg tctcctctgg ccctgagcca ggccgcgcct
     2161 tcccgcgcct ccgcagccgt cgactcgctc cccaaagtca agaaaatcac gcaggtcgcg
     2221 atttctcccg acggaacgca ggtcgcctac atcgtagacg gccatctgac tgtggtttcc
     2281 cttgccgaca acgctacgca cgcgatcgag gtcgaaggca agctgcctct gcgcagcgtc
     2341 gcgtggaaaa gcgattccaa aaccgtggcc ttcgttgcag atctccccgg caatgcgcct
     2401 tccgcgcaaa tttggaccgc ctcatccgat ggcagttccc cgtcgaagcg cgccgagctg
     2461 aagggtaacg tcgacgcgtc cagtttttcc ccggacggat cgaggcttgc catcctgttc
     2521 attgaagaca tgccgcgtaa agccggcccg ctcgagccca tgacgccgct cgcgggcgtg
     2581 gtcggcagca aggtttatga acagcgcatc gcgaccatcg acctggctgc aaaccaactc
     2641 acgcaaatca cgccggcgga catgtacgtc tatgagtacg actggacgcc cgacggccgc
     2701 gcctgggtcg ccgccgcggc ccacggcgac ggcgatgcaa attggtatgt cgcacgcctc
     2761 taccagttcg atgcacactc cggcgagcct cgcgaaatct acaaaccgaa actgcagatc
     2821 gccagcccgc aggtttcgcc cgacggcaag aacgtggctt ttattgaagg gctcatgagc
     2881 gacgagggcc caaccggggg cgacgtgttc gtggttccca tcgcgggtgg cagcgcgcgc
     2941 aacctcacgc ccaatcgcaa cgcttcgcct tcggcggtgt actggcgcga cgccagccat
     3001 atcgtgttcg ccgaaatcgc cgatggacac tctgccttcg ccaaggtcag caccgccgga
     3061 ggcacgccgc agacgctttg gagcggcgag gagttcattg gcacagactc gacaccgacc
     3121 gcatcttttt cccacgatag tgccgtaagc gcagtcgttc gggaatctgc caatactgca
     3181 cccgaggttt gggctggcgt cctcggccac tggaagcaga tcagcacggc caatgcagac
     3241 gccaaaccag cgtggggcga aactcgaaac tttcactgga acaacggaaa aacacgggtc
     3301 cagggtatgt tgacgttgcc gaatgactat gacgcggcca agacctaccc gctggtcgtc
     3361 agcgtgcacg gaggcccatc atcggcctgc cagccgcgct ggaacgagcg cgaaatcggc
     3421 gccatgtcgg ccatgggcta tttcgtgctt tgcccgaacc cgcgcggcag ctacgggcga
     3481 ggcgagacct tcacgcaagc gaacgtgaag gatttcggag gaggcgactt tcgcgacatc
     3541 atggctggaa tcgacgcggt ctccaaagat tttccaatcg acccgcagcg catcggcatt
     3601 cgcggacaca gttatggtgg gtacatgacc atgtgggcag agacgcaaac gcatcgtttt
     3661 gccgctgccg tcgcgggcgc cggactttcc gactggctga gctattacgg agtcaacgag
     3721 attgacgagt ggatgatccc tttcttcggt gcctcgattt atgacgatcc cgccgtctac
     3781 gccaagagcg accccatgca ctttgtgaaa tcggttcaaa cgcctacgtt gattctggtc
     3841 ggcgacagtg acggcgaggt tccgatggag cagtccgtcg agtggtggca cgccctcaac
     3901 accatgaaag tgccaaccgc gctggtggtc tatcctcacg aagggcacat cttcggaaac
     3961 cctgatgact cccgcgacta cactttgcga acgctggaat ggtttgagga atggttcgcg
     4021 aaggccgcaa gctcagccaa gcgctaaacg gaattgtccg aacctaccac acggatcttg
     4081 cgcctggtct ctccttcgcg ctcgagggaa atctcgacat cgcattcccg cagtaaccgc
     4141 ggaaatttct cgccccactc gatcagcagg atgccgtcgt cggagcgcag gtcatcgagc
     4201 gcgagcgtct ccagctcgcg tggtgtgtct acgcggtaaa ggtcgatgtg ataaagactc
     4261 gcccgcggcc cgcgatattc gtgcaccagc gtgaaggtag gactagtgac gtcttcctca
     4321 gcagccgcct cgaatgccgc cgcgatccct ttaaccaggg tagtttttcc ggcgcccagc
     4381 tcgccgcgca gcagcaccag cttcggcggt gcaagcaatt ccgcaagcgt ccggccaaaa
     4441 gcgatcgtct cctcggccga gcgcgtggtg atctctctca ggacggtctt cgctgtggcc
     4501 tgatcggaca cacgatcagt ataaagtgga agagtcgccg caggaggcgt ggaagagcgg
     4561 cctttcaagg ccgcgtgagg ggcaacaaaa attgtcgccc tttagggctg gtggacgcgg
     4621 ccctggaggg ccgctcttcc acgacgccag aggtagagct gctcccgcgg cgctagtgcg
     4681 tggagaacgc gctcgacttc aatccgagca cacttctttc cgcgacgtga tcaagcgacg
     4741 tgcgcaggcg gcgatgatcg tcatcctcga gcgccacgaa gcggaacggc tgcagcacgc
     4801 cgtcggtcgt cttccgcatg gggctgagga cttcggccat gccgtgcaca ggtccggcct
     4861 gcgtctggaa ggcgacctcc acgaagtcgc cctgtcccag cgattgcgcg atgcgcagca
     4921 ggccgccggt cacggaaatg gtttggagtt tagctttggt gcgccggcca tcttctaacc
     4981 gaatcgccgc caggacggaa tctcccagct ggattcgggc cgcgcgccgc gatgggtgcg
     5041 gttgggggaa gtgggtcata agccaactgc actatggcac tatcccctgt tgcgtaatag
     5101 attactgagg cccatcgtcc gaattccgca tgggaggcct gaactga
//
LOCUS       DASPUL010000289         3825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_323343, whole genome shotgun sequence.
ACCESSION   DASPUL010000289 DASPUL010000000
VERSION     DASPUL010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323343"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VEJ00_07095"
     CDS             <1..582
                     /locus_tag="VEJ00_07095"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXX00962.1"
                     /translation="RAYSIESLPQIRERMREPVAVGAVAGAGRMMATWPEGVDVSPAA
                     LLALLNHQLYESTPPEKYATLFLGIYDGRAHRLTYSNGGHLPPILIGESGGIRKLETG
                     GTVVGLFDNMNYAEGAVDMHPGEIFVAYSDGVTEPENDFGEFGEARLIDLVRENRYLP
                     LAQISQIVTTAVDDWIGDKEQPDDVTLVLARSR"
     gene            821..2146
                     /locus_tag="VEJ00_07100"
     CDS             821..2146
                     /locus_tag="VEJ00_07100"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HXX00963.1"
                     /translation="MKKGKTAVIVGAQWGDEGKGKIVDVLSENFSVVARYAGGHNAGH
                     TVIINGKKFILQLVPCGVLRPGCRSVIGNGVVLDPLAFLKEVGALREAGVQVDGNLFV
                     SNRAHVILPYHRMIELAAENAPGRVKIGTTSRGIGPAYEDKMGRRGLRVADLLDLQLL
                     KTHIENAVREKNMIAHALFNSEPVDADKMYAEYAQAAEKVAPFVGDTAVLLNQALARG
                     ESIVFEGAQGTMLDIDHGTYPFVTSSSATSGGAVIGTGVAPNAIDSVIGITKAYCTRV
                     GEGPFPTEEKGLAGEGLRKRGNEYGAVTGRPRRTGWLDLPLLRYSGMINGTSWLVVTK
                     LDVLDDLAEIPVCVGYKIDGKQTAEVPAQASGYDKIECVYQKIPGWQTSTQGITEYGK
                     LPQRARDYLAFVEKEAGAKIGMISTGPDRDHTILLDEFASELKTAAKKA"
     gene            2189..2476
                     /locus_tag="VEJ00_07105"
     CDS             2189..2476
                     /locus_tag="VEJ00_07105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HXX00964.1"
                     /translation="MVVLAVTWMAKVGHETEVAALFEKLTEQSRKEPGCAMYQVHRHK
                     TEPRRFFIYELYKDDAALEAHRTTSHFLQFAKKDLPKVADRVEGHLFEPLG"
     gene            complement(2528..2716)
                     /locus_tag="VEJ00_07110"
     CDS             complement(2528..2716)
                     /locus_tag="VEJ00_07110"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HXX00965.1"
                     /translation="MPLVQITMLAGRTADQKRKIAKRITDVLAEEAGARPEAVVIAFH
                     EVSKESYAHGGELMADKK"
     gene            complement(2802..3227)
                     /gene="ndk"
                     /locus_tag="VEJ00_07115"
     CDS             complement(2802..3227)
                     /gene="ndk"
                     /locus_tag="VEJ00_07115"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXX00966.1"
                     /translation="MKTLQRTLTIVKPDAVRKNAIGDIIEQFEKNGFRILAMKMLEIS
                     KHQAEQFYAVHASRPFFNTLTDFMSSGPIVVLALEKENAIADLRKLMGATNPANAEEG
                     TIRKKWATNIEHNAIHGSDAEDTARFELSFFFAGYELAK"
     gene            complement(3344..>3825)
                     /locus_tag="VEJ00_07120"
     CDS             complement(3344..>3825)
                     /locus_tag="VEJ00_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXX00967.1"
                     /translation="KIGIMPASIHKEGSIGIVSRSGTLTYEAVHQLTQRGIGQSTAIG
                     IGGDPIIGTNFVDALDLFNRDPETEAVIMIGEIGGNAEETAAEFVKTHMKKPVVGFIA
                     GQTAPPGRRMGHAGAIISGGKGTAAEKIKALEAAGIRVAKSPAAIGETLAAALGVSV"
BASE COUNT          762 a         1140 c         1164 g          759 t
ORIGIN      
        1 cgcgcctaca gcattgagag cctgccccaa atccgcgaaa ggatgcggga gccggtggcc
       61 gtgggagccg ttgccggcgc cgggagaatg atggcgacct ggcctgaagg cgtggacgtt
      121 tcgccggcag ctttactcgc cttgctcaac caccagctct acgaaagcac cccacccgag
      181 aaatatgcca cgttgttcct ggggatctat gacgggcgtg cgcaccgcct gacctacagc
      241 aacggcggcc acctgccccc tatcctgatc ggcgagagcg gcggcattcg caagctcgaa
      301 accggcggca cggtagtggg cctgttcgac aacatgaact atgccgaggg cgcagtcgac
      361 atgcacccgg gcgagatttt cgtcgcttac agcgacggcg tcacggagcc ggaaaatgat
      421 ttcggtgaat ttggcgaggc ccggctgatc gacctggtgc gagagaatcg gtacttgccg
      481 ctggctcaaa tcagccagat cgtgaccaca gccgtcgatg actggatcgg cgacaaagaa
      541 cagccggacg acgtcaccct ggtcctggcc cgaagcaggt gaaatcggcg aagggttagc
      601 ctggccgaac gccgccaaaa aatcgacaaa taggctgcga aaccgccaga tcgcctgtcc
      661 attgctattg ttcgcgaggg cacgctaaga tagctcgtac acatcttctc cgctcacaat
      721 cccagtaact ttgcgaggta tccccgcggg tgaggagtgt gcgactccgg ggcagcgcgg
      781 tcctttgtgt gcctgaaact ccccccggac ggagccggat ttgaaaaaag gcaaaactgc
      841 ggtgatcgtt ggcgcccagt ggggcgatga gggcaagggg aagatcgtcg atgttctgtc
      901 ggaaaacttc tccgttgtag cccgctatgc cggcggacac aacgccggtc acaccgtcat
      961 catcaacgga aagaaattca tcctgcaact ggtgccttgc ggcgtgctgc gacccggctg
     1021 ccgcagcgtc atcggcaacg gcgttgtgct cgatccgctg gcgtttctga aagaagttgg
     1081 agccttgcgc gaggccgggg tgcaggtcga cgggaatctt ttcgtttcta accgtgcaca
     1141 cgtaatcctc ccctaccacc gcatgatcga actggcggcg gagaacgcgc ccgggcgtgt
     1201 aaagatcggc acaacatcgc gcggcattgg tcccgcctac gaagacaaga tggggcgccg
     1261 gggcctgcgc gtcgccgatc tgctcgatct gcagcttctg aagacgcaca tcgagaatgc
     1321 ggtgcgcgag aagaacatga tcgcgcacgc gttgttcaac tccgaaccgg tggacgccga
     1381 caaaatgtac gccgaatatg cccaggcggc ggagaaagtt gcaccgttcg ttggcgacac
     1441 cgccgttctg ttgaatcagg cgctggccag aggcgaatcg atcgtgttcg aaggcgcgca
     1501 gggaacgatg ctcgacatcg accacggtac gtacccgttc gtgacctcct ccagtgcgac
     1561 gtcaggcggc gcggtgatcg gcaccggcgt cgccccgaac gcgatcgact cggtcatcgg
     1621 catcaccaag gcatattgca cgcgcgtcgg cgagggtcca tttcccaccg aggaaaaagg
     1681 cttggccggc gaaggcctgc gcaaacgcgg caacgaatat ggagctgtga ccggacgtcc
     1741 ccggcgcaca ggatggctcg atctgcctct tctccgctac tcgggcatga ttaacggcac
     1801 ttcgtggctc gtggttacca agctcgacgt gctcgacgac ctggccgaga ttccagtctg
     1861 tgttggctac aagatcgacg gaaagcagac cgccgaagtg cccgcccaag ccagcggcta
     1921 cgacaagatc gagtgcgtat atcagaaaat ccctggctgg cagacctcaa cccagggcat
     1981 taccgagtac ggcaaactgc cgcagcgggc gcgagactac ctcgcgttcg tggagaagga
     2041 agccggggcg aagatcggca tgatttcgac cggccccgac cgcgaccata cgattctgct
     2101 cgacgagttt gcctctgagt tgaagacggc cgccaagaaa gcctagaatg tcctccccgg
     2161 tacagcctgc cgggggagga gcgagcgaat ggtggtgctg gcggttacct ggatggcgaa
     2221 agttggtcac gaaaccgaag ttgccgcgct gtttgagaag ctgaccgagc aatcccgcaa
     2281 ggaaccgggc tgcgccatgt accaggtgca tcggcacaag actgagcccc gtcgcttctt
     2341 catctacgaa ctgtacaagg acgacgccgc ccttgaggct catcgcacga cttcacactt
     2401 tctgcagttt gctaagaaag acctccccaa ggtcgccgac cgtgtggaag ggcatttgtt
     2461 cgagccgctg ggatgagtgt ggcgcgggcg ctcccgcccg cgaacgcttg agctcgcgtc
     2521 cgtgtcgcta cttcttgtct gccatcaact cgccaccgtg ggcgtagctc tccttcgaaa
     2581 cttcgtggaa agcgatgacg accgcctcgg gtcgtgcgcc ggcttcttcg gccaggacat
     2641 cggtaatccg cttggcgatc ttgcgcttct gatcggctgt gcggcctgcg agcatcgtga
     2701 tttgaactag aggcatagag gagtacccag tacttagtac tcagtaccca gtgaaaccct
     2761 cggtaccaat agactgggta ctgggtactg gctactgagt actatttcgc cagttcgtat
     2821 cccgcaaaaa agaagctgag ctcgaaccgg gcggtgtcct ccgcgtcgga accatgaatc
     2881 gcattgtgtt cgatgttggt cgcccacttc ttgcggatgg tgccttcctc ggcgttggcc
     2941 gggttggtgg cgcccatgag cttgcgcaga tcggcgatgg cgttttcttt ttcgagggcc
     3001 agcacaacga tggggccgct cgacatgaaa tcggtcagcg tgttgaaaaa cgggcggctg
     3061 gcatgcacgg cgtagaactg ctcggcctgg tgcttggaga tctccagcat cttcatggcg
     3121 agaatgcgaa atccattctt ctcgaattgt tcgatgatat cgccgatcgc gttcttgcgc
     3181 accgcgtcag gcttgacgat ggtaagagtg cgttgcagag tcttcatgaa gtccttagtc
     3241 gttagtcctt agtcgttagt cgtgcacggt taggcgtcgg ccggaagggt ttgtcattcc
     3301 gagcgtagcg aggaacctgc tttcttgttg ccaggcaccc gcactacacg ctcacaccca
     3361 gcgccgctgc aagggtttcg ccgatcgctg ccggcgattt tgccacccgg atgcccgccg
     3421 cttccagcgc cttgatcttc tccgcggccg tgcctttccc gcccgaaatg atcgcaccgg
     3481 cgtggcccat gcgacgcccc ggaggcgcgg tctgtccagc gatgaatccc acgactggct
     3541 tcttcatgtg ggtcttcacg aactcggctg cggtttcctc ggcattgccg ccgatttctc
     3601 cgatcatgat tacggcttcg gtctcgggat cgcgattgaa caagtcgagg gcatcgacaa
     3661 aattcgtgcc gatgatcgga tccccgccaa tgccaatcgc ggtcgattgt ccaatgccgc
     3721 gctgcgtgag ctgatgcacc gcctcgtacg tcagcgtgcc cgaacgcgaa acgatgccga
     3781 tggatccttc cttgtggatc gatgccggca tgatgccgat cttgc
//
LOCUS       DASPUL010000290        13841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1829286, whole genome shotgun sequence.
ACCESSION   DASPUL010000290 DASPUL010000000
VERSION     DASPUL010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13841)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1829286"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VEJ00_07125"
     CDS             <1..328
                     /locus_tag="VEJ00_07125"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX00968.1"
                     /translation="MNFGDLMSHIATTNYQFCAGLKDTDTSQLPSPTDKDGIVKFLSD
                     SFAYCSGIISTLTDDQLSKRHNSPDGNLPGRDVLLAMYVHVAHHRGQAEIYLRDKGIK
                     PPSYRV"
     gene            complement(423..2297)
                     /gene="glmS"
                     /locus_tag="VEJ00_07130"
     CDS             complement(423..2297)
                     /gene="glmS"
                     /locus_tag="VEJ00_07130"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXX00969.1"
                     /translation="MCGIVGYVGRKSVVPVIIDGLRRLEYRGYDSAGIAVGGNGDGLQ
                     IRRAEGKLRNLEEAVRQKPLDGTYGIGHTRWATHGRPTEENAHPHRDCTGKIVVVHNG
                     IVENYLSLKKKLAEEGHKFTTETDTEIIAHLIEKYSATSGNGRRPTLEEAVRHALKQL
                     SGVFALAVISTDDPNKIVAARNGPPAVIGLGNDEYFVASDVPAILSHTRDIFFLADGD
                     MAVVTPEGVQLSDFDGKPVQRAVQHVTWDPIMAEKGGFKHFMLKEIYEQPRAVRDTTL
                     GRVSQDTGHIFLEEMEISEAEFRGAQKINIIACGTSWHAGLAGKFMIETLARVPVEVD
                     YASEWRYRSPIVDPQTITLVISQSGETADTIAAQREAKARGSKTLAICNVVGSMITRE
                     AAGTIYTHAGPEIGVASTKAFTGQLTALYIFAMYLAQVRGAMAPDQARAAMLELTRIP
                     AKLDHILTHDEACEELAKHYQRVHDFLFLGRGIHYPIALEGALKLKEISYIHAEGYPA
                     GEMKHGPNALIDENLPVVVIATRDLSNPGAVQRYEKTISNLKEVKARSGVVIALATDG
                     DEEIKEAADHVLYIPPAPEELSPILEIVPLQLLAYHIAVRRGCDVDQPRNLAKSVTVE
                     "
     gene            complement(2331..2576)
                     /gene="purS"
                     /locus_tag="VEJ00_07135"
     CDS             complement(2331..2576)
                     /gene="purS"
                     /locus_tag="VEJ00_07135"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HXX00970.1"
                     /translation="MTAYVYVSLKKSVLDPQGKTIQGALQKMGYRGLESVRQGKYFEL
                     TLDGGLSREEAQSEVERIAREVLTNPVIEEFRFSLSE"
     gene            complement(2681..3025)
                     /locus_tag="VEJ00_07140"
     CDS             complement(2681..3025)
                     /locus_tag="VEJ00_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00971.1"
                     /translation="MRCDGVRERVQTERSFDLRCAILPAKIANYNHRVARGWESKSVE
                     AQQAEAEEKASGLRRKLSAQEAERLREKEGLRLARQRVLHQMEQSTDPRHRALLGSAL
                     ADLDAKLSRLDD"
     gene            3222..5213
                     /locus_tag="VEJ00_07145"
     CDS             3222..5213
                     /locus_tag="VEJ00_07145"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXX00972.1"
                     /translation="MTSREFLSGLGDPIRFPFPETLTEPEKLLSAYFQSTTVGLCILD
                     SAFRFVAVNDKLAEINGIPAQDHVGKTVRQVLGDFADIVEPEFQRVLSTGETILNFQI
                     SALLPTRKEIGHWIDHYIPIKDATGTVTGIGIVVIEVTEQKKLEESFQSLNTRLRRER
                     ECLQMLSDVSNILTSNWNIPQVFPRISARLRRLLRHEYAGLSIQDHGTGLLIRQAEDF
                     PLGKGFLRATSVSAKDGPEYRCISTGAPGIFSAEEMQSFDADISSGFAAEGLRSLCCL
                     PLLRPKGPLGVLLLGSTRENAFRPEDLALLNQIAAQVAIALENHRVAAEIEALQRRFA
                     EERKLLQDEIPSEGQFAEIIGVSLVLKQVLEQVATVATSDATVLILGETGTGKELIAR
                     AIHRMSRRHDQKFIKVNCAAIPTGLLESELFGHEKGAFTGAISQKIGRMELADGGTLF
                     LDEVGEIPLELQAKLLRVLQDQEFERLGSTRTIKVNLRLIAATNRDLAGSVIEREFRS
                     DLYYRLSVFPIHMPALRERREDISLLVRYFVRKFALRMDRHIEAVPKETMNALMEWSW
                     PGNVRELENLIERSVILSQGRVLRVPLSELRRDSPPPASSPDQTLDRAEREHIIRVLR
                     EAGGTISGTGGAAARLGLKRTTLQSKMRRLKITRQDCSG"
     gene            5695..6663
                     /locus_tag="VEJ00_07150"
     CDS             5695..6663
                     /locus_tag="VEJ00_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXX00973.1"
                     /translation="MGSFSRPHPHDIDPLYLFACQVDWERREEPSAAWELISASQSSH
                     SDTRAHARALLAASRHVGGMGASSTLPVPAKPRPASDMEANMKAPYGLEIIENCNDCK
                     HTNPSFFCGFSRPVRQALNQVSHKSTLPAGVVLFVEGQNPRGVFVLCSGKVNLSTTSR
                     EGKILILKTAQAGEALGLSASISGVAYETTAETASPCQLNFVDRAHLLELLESHPEVG
                     VHAAQSLSRDFQNAYRDIHDLVLTRSSAGKLARLLLSQSPTQDADSVEVRIASSMTHE
                     EIAQRIGSSRETVTRLLSDLKRKHLIRLDGPTLVIRNRMALEALAV"
     gene            complement(6903..7682)
                     /locus_tag="VEJ00_07155"
     CDS             complement(6903..7682)
                     /locus_tag="VEJ00_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXX00974.1"
                     /translation="MSLSAIIVDDEQLARDELAYLLKSAGEVNVVAQGKNGLEAVHLI
                     KEHNPDLVFLDVQMPGLDGFGVIKKLLDKKIPLPKIVFATAYDQYAVKAFEVNAVDYL
                     LKPFDKKRVAQSLQKARAKQEADALPAEKIETLVRMIESQKQPASKVLLKALGRMFLV
                     DQKDICYAWIEDGVITVVTGGAGSMEGQSNCRTLEELLASLDTQLFWRAHRSYLVNIN
                     RIREVVPWFKSSYQLRMDDRKQTEIPVSRAQTKRLRELFGL"
     gene            8143..9141
                     /locus_tag="VEJ00_07160"
     CDS             8143..9141
                     /locus_tag="VEJ00_07160"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXX00975.1"
                     /translation="MKLSQKVVLSAVASLVLLAGMPALTHAQMSKVKTVFVIILENHN
                     WTGNDAGASFGDPDIKGNPLAPYINGTLLKTSAHAEQYFNPPGNHPSQPNYLWLQAGT
                     NFGVLADTQPGQPQLLTHKHLTKLLEEAGISWKNYAEPDFGSPSFDVCPLDFSFLDVE
                     HLATVYFNDVNDGLNPLSRECISHVVPYDYLATDLANNTLARYNLINPNLCHQGHEGI
                     SPCDSREPADNTLRSDQWLRKNVPLILESQAYKEGGALFITWDEAEDDGQFSDGPIPF
                     FLLSPFAKGGGKAEFSNSIHYDHSSTLKTIEEIFDVTPLLGHAADPETIDLSDLFK"
     gene            9221..10753
                     /locus_tag="VEJ00_07165"
     CDS             9221..10753
                     /locus_tag="VEJ00_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00976.1"
                     /translation="MTPTLVLSSRTNRRQRVHHRWTAAALACFLPAIAAQAQVTFTGA
                     RPNINLGSQAVGSRSATTGVPFMIAANTKVGSIAVLTTGGAGRDFAQAAGSACTATTF
                     AAATNCMVNVNFKPAVPGVRMGAVVFFSGPHNSGTVLASVPVFGLGTGPQLVFGPGGS
                     QTTVGSNFISPEGVAVDAGGDVYITDVGLQEVFKVTPAGKQATVGSGLQVPGGVAVDG
                     AGNVYIADSQAAAVFKVTPGGVQTTIGSGFSFPTSVAVDGAGNVYVSDPFIPTVFKIT
                     PAGMQTQVGSGYNTPAGVAVDAAGNVYVADTFSSVVFKVTPGGKQTTVGTGLVSPAAV
                     AVDAAGTLYIADDAANSLIEVTSAGVQTTAVEGLNVPGDVVLDGSGNLYVSNSFSSEV
                     VKINRASAPSLTFATTKINHTSTDSPKDVLIENIGNAKLNFSAVSFPADFRQSGATTC
                     ASGTSLSAGASCALAIEFSPVTPLSGKPSAVLTEAVTLTTNNLNVAKKLAQVSVNGKE
                     LP"
     gene            complement(10864..11961)
                     /locus_tag="VEJ00_07170"
     CDS             complement(10864..11961)
                     /locus_tag="VEJ00_07170"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HXX00977.1"
                     /translation="MPGPGKKRVLLVDDAVVVRKTLSDVIAQDPDLEVVGTAVNGRVA
                     LAKFSTFKPDIVLLDIEMPEMDGLETVRELRKLDRLVPIIMFSTLTERGASATLEALT
                     LGATDYVTKPSSVDVTAMTEAISRELVPKIRALCRLPEAATGAPVPKLQTQFSPPPIA
                     ARRQPALPVQVVAIGVSTGGPDALACVLPSLPADLKVPVVIAQHMPPIFTTLLAARLT
                     AKSSLRVRECVSGEALTPGSVVIAPGDFHMVVSQEDGVVRLRTHQGAKENFCRPSVDV
                     LFRSIAQVYGGRCLAVMLTGMGQDGLKGCEMLRGLGARIYVQDEATSIVWGMPGFVAR
                     AGLADKILPLEQIGAEIVRATTTSLTARTPS"
     gene            complement(11986..13692)
                     /locus_tag="VEJ00_07175"
     CDS             complement(11986..13692)
                     /locus_tag="VEJ00_07175"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXX00978.1"
                     /translation="MVSNLKLSAKLLGTIVAILAITSGISFWIMQNRANRQAEDAFRD
                     KLRQITGMASATRNWFAGNIDLMVPNRDFKHLEQVPVVVAMRTAEEYASKEGMKFRTP
                     SLHPRNPKNQATDFERRALEAFERDSTLAEFYDRAEIDGREIMRYAQPVRVEQDCLQC
                     HGDPAGQKDPFGYPKEGMKAGELRAAFVLEAPTDKLVQESRSNMMATFLVSFFTVLAA
                     IVVMYYVLRQLVIRPVKECAEFAEAIAAKNLSLDDLDVSSADEVGDAMTAMNTMKNNL
                     HAVVQAIADTSGHVASASAELSATSQQISANSEETSAQVGVVSQATQQVSQNLQSVST
                     GAGEMTTTIQSIASNAHEAASVASSAVQTAQAANNTISKLGESSAEIGEVIKVITSIA
                     QQTNLLALNATIEAARAGEAGKGFAVVANEVKELAKQTAKATEDISRKITAIQQDTKG
                     AVDAIGSIGTVINKVNDISSTIATAVEEQSATTNEMTRSVGDAAKGSAEITRNIEGVA
                     EAARGTSSSAQESQKAADELAEMAAQLRGLVAQFKIVAEGAVEAGPAAAPMRARAAHA
                     GR"
BASE COUNT         2618 a         4395 c         3922 g         2906 t
ORIGIN      
        1 catgaatttc ggcgatttga tgtcccacat cgccaccacg aattaccagt tctgcgcagg
       61 tctcaaggac accgatactt cccaactgcc ttcccctacc gacaaagacg gcatcgtcaa
      121 atttctgagc gattcgttcg cgtattgctc aggcatcatc tcaaccctga ccgacgacca
      181 gctgagcaag cgccacaact cccccgacgg aaatctcccc ggccgcgacg tgctgctggc
      241 gatgtacgtc cacgtggcgc atcatcgcgg ccaggctgag atctacctgc gcgacaaagg
      301 catcaaacca ccgtcgtacc gggtgtgaag acgagccgaa cggcactctg gggtcgatcc
      361 atttgtgata gagcggcgct tcagcgccgc gtcgagcggc agattgagag gggcggtagc
      421 tgttattcga ctgtaactga tttcgccaga ttcctcggct gatccacgtc gcagccgcgg
      481 cgcacggcaa tgtgataggc gagtaactgc aggggaacga tctccagaat cggcgacagc
      541 tcttccggcg ccgggggaat gtagagaacg tggtccgctg cttccttgat ctcttcatcg
      601 ccgtcggtcg ccagcgcgat caccacgccg gaccgtgcct tcacttcttt caagtttgaa
      661 atagtcttct cgtatcgctg tacggctccc gggttgctca aatctcgcgt cgcaattacg
      721 accactggca aattctcgtc aatcagcgca ttggggccgt gcttcatttc acccgccggg
      781 tatccttcgg cgtgaatgta ggaaatttcc ttcagcttga gcgcgccctc aagcgcgatg
      841 ggatagtgaa tgccgcgccc caggaagaga aaatcatgca ctcgctgata gtgcttggcc
      901 agttcttcgc aggcttcgtc atgcgtgagt atgtgatcga gcttcgccgg gatccgcgtc
      961 agttcgagca ttgcggcgcg ggcttggtcg ggagccatcg ctccccgcac ctgcgccaga
     1021 tacatagcga agatatataa cgcggtaagc tgcccggtga acgccttggt cgaagccacc
     1081 ccgatctccg gccccgcgtg cgtgtaaatc gtgcccgccg cctcccgcgt gatcatcgat
     1141 cccaccacat tgcaaatggc cagcgtcttc gatccccggg ccttggcctc gcgctgcgcc
     1201 gcaatcgtgt cggccgtttc gcctgactgc gaaatcacca gcgtgatcgt ctgcggatcg
     1261 acaatcggac tgcggtaccg ccattcgctg gcatagtcca cttccactgg cacccgcgcc
     1321 agcgtttcga tcatgaactt gccggccagc ccggcgtgcc agctggttcc gcaggcaatg
     1381 atgttgatct tctgcgcgcc gcggaactcg gcctcgctga tctccatttc ttccagaaag
     1441 atgtgcccgg tatcctgcga aactcggccc agagtcgtat cgcggacggc acgcggctgc
     1501 tcgtagatct ccttgagcat gaaatgcttg aagccgccct tttcggccat gatcggatcc
     1561 caggtcacgt gctgcaccgc gcgctgcaca ggttttccgt cgaagtccga cagttgcacg
     1621 ccctcaggcg tcactacggc catgtcgccg tccgcgagaa agaagatgtc gcgcgtatgg
     1681 ctcaggatgg ccggcacatc ggaagcgacg aaatactcgt cgtttcccaa tccaatgacc
     1741 gcgggcgggc cattgcgcgc tgccacaatt ttgttgggat cgtctgtgga aatgaccgcc
     1801 agggcaaaaa caccgctgag ctgtttgagc gcatgtcgca ctgcttcttc cagcgtcggc
     1861 cgacggccgt tgcccgaggt cgccgaatat ttctcgatca ggtgagcgat gatctcggtg
     1921 tcggtctcgg tcgtgaactt gtgtccttct tcggcaagct tctttttcag gctgagatag
     1981 ttctcgacga tcccgttgtg cacgaccact atcttcccgg tacagtcacg gtgagggtga
     2041 gcattctctt cggtaggacg tccgtgagtg gcccaacgcg tgtgaccgat accatacgtg
     2101 ccgtcgaggg gcttctgccg aaccgcttct tccaggttcc gcagcttgcc ctcggcgcgc
     2161 cggatctgca acccgtctcc gttgccgccc accgcgatcc ccgctgaatc gtagccgcgg
     2221 tattccagcc gccgcaggcc atcaatgatg accggcacca cactcttcct gccgacgtaa
     2281 ccgacaatac cgcacatgcg gaagcctcac tggcgttccc cggtcgattc ttactccgac
     2341 agggaaaaac ggaactcttc gatcacgggg ttggtcagca cttcgcgggc gatccgctcc
     2401 acttcactct gggcctcttc gcgcgagagc ccgccgtcga gcgtaagctc gaaatacttc
     2461 ccttgacgca cactctccag ccccctgtag cccatctttt gaagtgcgcc ctgaatcgtt
     2521 ttgccttggg ggtcgaggac acttttcttg agcgaaacgt agacgtaagc ggtcatgaac
     2581 aagtcattat acgtcacggt ctctggaacc gagattccgg aattgcatcg ctccagggcc
     2641 cgagacctca aatactgatt ctccccctcg cgcgagtgct tcaatcatcc agccggctca
     2701 gctttgcatc cagatccgcc agcgcgctcc cgaggagcgc acgatgccgc ggatccgtgc
     2761 tctgctccat ctggtgcagg actctctgcc gcgccagccg gagaccctcc ttttcccgga
     2821 gcctctctgc ctcttgcgcg ctcagtttgc gacgcaagcc ggacgctttc tcttctgcct
     2881 ccgcctgctg tgcctccacc gatttgctct cccatcctct ggccacccgg tgattatagt
     2941 tcgcgatttt tgctggcagg atcgcgcaac gcagatcgaa cgaccgctcg gtttgcacgc
     3001 gctctcgcac cccatcgcag cgcatttcgg gaagcgtacc ggatatcgca ctcggcactt
     3061 ccgcgcttga ctctctgggt ataatctgtg gtcagttccg ggataggaca ggagtgcctt
     3121 gagggggagg caacctgggg agtgtcagtg ttcccctcga gcccgcaaca gacgattcct
     3181 cgtcgagtct gcattcccat tccccaccag cgaggtgtcc tgtgacgtca cgggagttcc
     3241 tctccggact cggggatccg attcgctttc cttttcccga aactcttacc gaacctgaaa
     3301 agttgctctc cgcatacttc cagtccacca ccgtcggact ttgcattctc gactccgcct
     3361 tccgttttgt cgcggtcaac gacaaacttg cggaaataaa tggtatcccc gcacaggatc
     3421 atgtgggcaa gacggttcgc caggtgctgg gcgactttgc ggatattgtc gagccggaat
     3481 ttcagcgtgt gctctccact ggtgaaacaa tcctgaactt ccagatttcc gccctccttc
     3541 cgacgcgaaa ggagatcggc cactggattg accactacat cccgatcaaa gatgccaccg
     3601 ggacggtgac cggaatcggc attgtggtca tcgaggtgac cgaacaaaag aagctggaag
     3661 agtcgttcca gagcctcaac acgagactgc gccgcgagag ggaatgcttg cagatgcttt
     3721 cagacgtcag caacatcctc acgtcaaact ggaatattcc ccaggttttc cccaggattt
     3781 ccgctcggct tcgacggctt ttgcgacacg agtacgctgg cctctcgatc caggaccatg
     3841 gcaccggatt gctgatccga caagccgaag atttcccctt ggggaaagga ttcttgcggg
     3901 ccacttcggt cagtgcgaag gacggtcccg aataccgttg tatctccacc ggcgctcccg
     3961 ggattttctc tgctgaggag atgcagagtt ttgacgccga tatctccagc ggtttcgccg
     4021 ctgagggact gagatcgctc tgctgcttgc cgctgctgcg ccctaaaggt cctttgggag
     4081 tccttttgct gggcagcacg cgcgagaacg cctttcggcc ggaggacctc gcactcttaa
     4141 accagattgc cgcccaggtt gccattgctc tcgaaaatca tcgggtcgcg gcggagatcg
     4201 aagctctcca gcggcgcttc gccgaggaaa ggaagcttct gcaagatgag attccatcgg
     4261 aaggtcagtt cgcggagatt atcggggtca gtctcgtgct caaacaggtt ctcgagcagg
     4321 tggcgaccgt tgccaccagc gatgcgaccg tactgatcct gggggagact ggaaccggga
     4381 aggaattgat tgctcgcgcc atccaccgca tgagtcgccg tcacgaccag aaattcatca
     4441 aagtaaattg tgccgcgatt cccacgggct tgctggagag cgagctattt ggccacgaga
     4501 aaggcgcctt caccggggcg atcagccaga aaatcggccg catggagctg gccgacgggg
     4561 gcactctctt cctcgacgaa gtcggcgaga ttccgctcga actgcaggcc aagctcttgc
     4621 gcgtgcttca ggatcaggag ttcgaacgcc tgggcagcac gcgtacgatc aaagtcaacc
     4681 tgcgcctcat cgccgccacc aatcgtgacc tggcgggcag tgtgatcgag cgcgaattcc
     4741 gctctgatct ttactaccgc ctcagtgttt ttcccataca catgcccgcc ctgcgcgagc
     4801 ggagggaaga catctccctg ctggtccgtt acttcgtacg caagttcgct ctgcggatgg
     4861 accgccacat tgaagccgtt ccgaaagaga cgatgaacgc tttgatggaa tggtcctggc
     4921 ccggcaacgt gcgcgaactg gagaatctca tcgaaaggtc ggtcatcctg agtcagggca
     4981 gggtgttacg ggttcctctg agcgaactcc gtcgcgactc gccgccgccg gcgtcctcgc
     5041 cggaccaaac tctggatcgc gccgagcgtg agcacatcat tcgcgttcta cgcgaagccg
     5101 gaggcacgat ctccggaacc ggcggcgctg ccgcccggct gggcttgaag cgtacgactc
     5161 tccaatcgaa gatgcgacgc ctgaagatca cacgtcagga ctgctcaggc tgaccacgac
     5221 cctggtttcc ctgtaccgca tccgggtcac gtgtgtgact catgccgacg attgctaatc
     5281 ggcggatcgc cgagaattcg gcaggattcc aggaatagat cgcccgcttc tgtttcggaa
     5341 ctcgttccaa acagggaagt tcctaacctc gttctccgct tcctcatttc ggaattccaa
     5401 ttgcacaact tccgctcact ctgcctctgg ccggtcagag atcggacctc ttgcagagca
     5461 gcccttcccc aaaatcaaag aagctgggag taagcgatcc cggccgtgtg gagtcggaga
     5521 gtgttgttaa ccagcagaag tcaactgttc agcccggttc gatcggaagc tcttgacatc
     5581 gtagcgccaa gacttggggt ccatagttcg ccttgcgatt gcaatctcac ttcgggatgg
     5641 aacacatcgt ccgcgggcga gtgctccgct gaatccgcgc cctgcgaaca atctgtggga
     5701 tccttttccc ggccccaccc gcacgatatc gaccctcttt acctgttcgc ctgccaggtc
     5761 gactgggagc gacgcgagga acccagcgcc gcatgggaac tcatctcggc ttcgcagagc
     5821 tcgcacagcg atacccgtgc ccatgcccgc gccctgctcg ccgcttcccg ccacgtgggc
     5881 ggaatgggcg cgtcatcgac tttgccggtc cctgccaagc ccaggccggc gtccgacatg
     5941 gaggccaata tgaaagcgcc ctacggactt gagatcatcg aaaactgcaa cgattgcaag
     6001 cacaccaacc cttccttttt ctgcggattt tcacgtccag tacgccaggc gttgaaccag
     6061 gtcagtcaca agagcacctt accggccggg gtcgtcctgt ttgtggaggg ccagaacccc
     6121 aggggcgtgt ttgttctgtg ctcgggaaaa gtgaatctct ccacgacctc acgcgaaggc
     6181 aagattctca tccttaagac agcgcaggcc ggagaagccc tgggtctcag cgcgtcgatc
     6241 tcgggagtag cctacgaaac cactgccgag accgctagtc cctgtcagct gaatttcgtt
     6301 gaccgcgcgc acctcctcga gcttttggag tcacaccccg aagtaggcgt gcacgccgcg
     6361 cagagtctga gccgcgactt tcagaatgct tatcgcgata tccacgacct ggtgctgacc
     6421 cgctcgtcgg cgggaaagct ggcacgcctg ctgttgtcgc aatcgcctac acaggacgcc
     6481 gactcagttg aagtccgcat cgcatcctcc atgacgcacg aagaaatcgc acagcggatc
     6541 ggctcttctc gcgaaaccgt aacccggctc ctgagcgacc tcaagaggaa gcatctgatc
     6601 cgcctcgacg gacctacgct agtaatccgc aaccggatgg ctttggaggc tctggcggtc
     6661 taatcttccc cctgagaccg gaggcgggcc aggcagttcg gccttcctcc ggttgttttt
     6721 gtggaatccg tgccgttagc gatgagcgaa gggaatcgat gcaggcctct aatctgaccg
     6781 atgacctgct ggtcgccgtc gaacgggccg agaaagattg agcgcgcccg cagtcccaga
     6841 cggccggcac tgcgccatgc ccagtgccgc attcgcctct ggccgagagc caggacgata
     6901 gttcaaagtc cgaatagctc gcgcagccgt ttggtctgag cccggctcac gggaatctcg
     6961 gtctgcttgc ggtcgtccat gcgaagctgg taagaactct tgaaccaggg caccacctcg
     7021 cggatgcggt tgatgttcac caggtacgac cggtgcgccc gccagaaaag ctgcgtgtcc
     7081 aggctggcca gcagttcttc cagcgtccgg cagttcgatt gcccttccat gctgccggcg
     7141 ccgcccgtga ccacggtgat cacgccgtct tcaatccacg catagcagat gtctttctgg
     7201 tccaccagaa acatccggcc cagcgccttg agcagaacct tcgaggctgg ttgcttctgt
     7261 gattcgatca tccgcaccag agtctcaatc ttttcggccg ggagggcgtc ggcttcctgc
     7321 ttggctcgcg ccttctgcag cgattgggcg acgcgcttct tgtcgaacgg cttcagcagg
     7381 tagtcaaccg cgttcacttc gaacgccttc accgcatact gatcatacgc agtcgcgaac
     7441 acgattttgg gcagagggat cttcttgtcg agcagcttct tgatgacgcc aaacccgtcc
     7501 agccccggca tttggacgtc cagaaatacc agatcggggt tgtgctcctt gatcaggtgg
     7561 accgcctcga gcccgttctt gccctgcgca acaacattca cttccccggc gcttttgagc
     7621 agataggcca actcgtcgcg cgcgagttgc tcgtcatcga cgatgatggc ggagagagac
     7681 attccttgcc ccgggctaac agaggagtta acaatgaaaa gtatagtagc cgttttttgg
     7741 cagcgtcaaa ccagccgcca caacgcctct ccagaacagg cgaatcggcg cagcacagcg
     7801 gagggggtac agtcttcgac gcggtgttgg gttaccgttt gcccgctcgt ttcacgcaat
     7861 gaaattctca tcggtgttta ccgaaaattg acaatcctgt cgttgacttc caatgcggaa
     7921 cgcgcctagt ctgctcacaa tccgtacagt agtaacaccc cattttccga accgggcaac
     7981 ggtcctaacg aactgatctt gttctgttcg gtctgtagtc ccgccgtctc aaccgatccg
     8041 cagaatctgg cacacggcgg acgtgtgtcc gttcgctttc ggtccctgtc ttcgatgatt
     8101 caccggcttt ggccgcagtt aaatcgcacg gttgagggtg ctatgaaact ttcgcaaaag
     8161 gtagtgcttt ctgcagttgc ttcgttggta ttgctggcgg gaatgccggc gctgacccac
     8221 gcacaaatga gcaaagtaaa gactgtcttc gtgatcattc tggagaatca caactggacg
     8281 ggcaacgacg cgggagcctc gttcggagac ccggatatca aaggcaaccc gctggccccg
     8341 tatatcaacg ggacgctatt gaaaacgtca gcccatgccg agcaatattt caatccaccc
     8401 ggtaaccatc cgagccagcc gaactatctg tggctgcagg ccggaaccaa tttcggcgtc
     8461 ctcgccgata cgcagcctgg ccagccgcaa ctgttgacgc acaagcacct gaccaaactt
     8521 ctggaagagg ccggcatctc ctggaagaac tacgccgagc ccgatttcgg cagtccgtcc
     8581 ttcgacgtct gccctctcga tttctccttc cttgacgtcg aacacctcgc cactgtctat
     8641 ttcaacgacg tcaacgacgg cttgaatccg ctttcccggg agtgcatttc ccatgtcgtt
     8701 ccctacgact acctggcgac cgacctggcc aacaacaccc tggcgcgtta caacctgatc
     8761 aaccccaacc tgtgccatca gggacatgag ggcatttctc cctgcgattc tcgtgaaccc
     8821 gcggacaata ccctccgcag cgatcagtgg cttaggaaaa acgtccccct catcctggag
     8881 tcgcaggctt acaaagaagg cggcgccctg ttcatcacct gggacgaggc cgaagacgac
     8941 ggccagttca gcgatggccc cattccgttc ttcctgctct ccccgttcgc caaaggagga
     9001 gggaaagcgg aattcagcaa ttcgattcac tacgaccaca gctccacgct caaaaccatc
     9061 gaagagatct tcgatgtaac gccgctgctt gggcacgctg ccgatccgga gaccatcgat
     9121 ctcagcgact tgttcaagtg aggcagggaa tgctgtcggt cccgtccacc caatcgacaa
     9181 accaattgac aaatgaatcg acgaaacatt ggagaccttg atgacaccga ctcttgttct
     9241 ttcttctcgc acaaaccgac gccagcgggt ccatcatcgc tggacggccg cggctctggc
     9301 ctgtttcctg ccggcgattg cggcgcaggc gcaggtaacc ttcaccggag cacggcccaa
     9361 cataaacctc ggctctcagg ccgtcggatc gcgcagtgca actaccggcg tgccttttat
     9421 gattgcagcc aacacgaaag tcggcagcat tgccgtcctg accaccggcg gcgcaggcag
     9481 agactttgcc caggcggccg gcagcgcctg cacggccacc actttcgccg ccgctacaaa
     9541 ttgtatggtc aatgtgaact tcaaacccgc ggtgcccggc gtgcgcatgg gcgcggtggt
     9601 gtttttctcc ggcccacaca attccggtac cgtgttggct tcggtccccg tcttcggcct
     9661 cggcaccggg ccgcagttgg tctttggacc gggtggctcc cagactacgg tgggcagcaa
     9721 cttcatcagt ccagagggcg tcgctgtcga tgccgggggt gacgtctaca tcaccgatgt
     9781 cggcctgcag gaagtcttca aggtcactcc cgccggcaag caggcgacgg tgggctcagg
     9841 gcttcaggtt cccggtggcg tagccgtgga cggagccggc aacgtctaca tcgccgacag
     9901 ccaggcggct gccgtattca aggtgacccc gggcggagtg cagacgacga tcggctcggg
     9961 cttctcgttt ccaaccagcg tggctgtaga tggagccgga aacgtgtacg tgagcgatcc
    10021 ctttatcccg acggtcttca agattacgcc tgcgggcatg cagacccagg tcggcagcgg
    10081 atacaacacg cccgccggag tggccgtcga tgcggccggc aatgtgtatg tggccgatac
    10141 tttcagctcg gtcgtcttca aagtcactcc gggcggcaag caaaccaccg taggcacggg
    10201 cctcgtcagc cccgcggccg tggctgtcga cgctgccggc acgctctaca tcgccgacga
    10261 tgctgccaac agcctgatcg aagtgacatc agctggcgta cagaccacgg ccgtcgaggg
    10321 actcaacgtt cccggcgacg tagtgctcga tggctcggga aatctttatg tgtccaacag
    10381 cttcagttcg gaagtggtga agatcaaccg cgccagcgct ccgtcgctga ccttcgccac
    10441 tacgaaaatc aaccacacca gcaccgacag cccaaaggac gtgttgatcg agaacatcgg
    10501 caacgcaaag ctgaactttt ccgcagtcag cttcccggcc gacttccgcc aaagcggcgc
    10561 gacgacctgt gcctcgggta cgtcgctgtc ggctggagca agctgtgcgc tggcgattga
    10621 gttcagcccc gtgaccccgc tgagcggcaa gccctctgcc gtattgaccg aggcggtcac
    10681 gctcaccacc aacaacctca acgtcgcaaa gaagctcgct caagtcagcg tcaacggcaa
    10741 ggaactcccc tagcgaatgt tcgaggaaca gcgagaagcc ctttggggaa tgaacctaaa
    10801 gggctttgtt tttggcaggc tcggcgggat gggaggtgat gccgtcgatc cctgtcaccg
    10861 ttttcaggaa ggagtgcgcg cggtgaggga tgttgtggtt gctcgcacga tctcggctcc
    10921 tatctgctcc agcggcagaa ttttgtcggc cagcccggcg cgcgccacaa atccgggcat
    10981 gccccagacg atgctcgtgg cttcatcttg cacgtagatg cgggcgccga gtccgcgcag
    11041 catttcgcat cccttaagcc catcctggcc cataccggtc agcatcaccg ccaggcagcg
    11101 gcctccgtaa acttgggcaa tcgaacgaaa cagtacatcg accgatggcc ggcagaagtt
    11161 ctcctttgcg ccctgatggg tccgcagtcg gaccacccca tcctcctggc tgaccaccat
    11221 gtggaagtcg ccgggagcga tgaccacact ccccggcgtg agcgcctctc ccgagacgca
    11281 ctctcgcact cttaaactcg attttgcggt gagcctcgca gccagcagtg tcgtgaagat
    11341 tggaggcatg tgctgcgcga tgactacggg aaccttgagg tccgcgggga gagaaggcag
    11401 aacgcaggcc agcgcgtccg gaccgccggt cgaaactccg atcgctacta cctgtacggg
    11461 tagcgccggc tgccgccgcg ctgcgatagg cggcggcgaa aactgggtct ggagctttgg
    11521 tacgggcgcg cccgtggccg cttcgggcag ccggcaaaga gcccggattt tcggaacgag
    11581 ctcgcgcgag atggcctcgg tcatcgccgt gacgtcgaca ctgctcggct tggtgacata
    11641 gtcggttgct cccagggtca gggcttccag tgtggcggag gctccgcgct cagtcagcgt
    11701 gctgaacatg atgatgggaa ccaggcggtc gagcttccgc aactcgcgca ccgtctccag
    11761 cccgtccatc tccggcattt cgatgtcgag caggacgata tcgggcttga aggtggagaa
    11821 cttcgccagc gcgaccctgc cgttcacggc cgtgcctaca acttccaggt cggggtcttg
    11881 cgcaataacg tcagagagcg ttttgcgcac aactacggca tcgtccacca gcagcacgcg
    11941 cttctttccg ggtccaggca agcagagcgt ccttcccgcg ttcgtctatc ggccggcgtg
    12001 ggccgcccgg gcacgcatcg gcgctgccgc cggccccgct tctaccgctc cctccgcgac
    12061 gatcttgaat tgggcgacca ggccgcggag ttgtgccgcc atctccgcca gctcatcggc
    12121 cgccttttgt gactcctgcg cactcgacga cgtcccccgc gccgcctccg ccaccccttc
    12181 gatgttccgc gtgatctccg ccgacccttt ggctgcatcg ccgacgcttc gcgtcatctc
    12241 attcgtggtc gcactctgct cctccaccgc cgtcgcgatg gtcgaagaaa tgtcgttcac
    12301 tttgttgatc accgtgccga tggacccgat ggcgtccact gctcccttgg tgtcctgctg
    12361 aatcgccgtg atcttgcggc tgatgtcttc ggtggccttg gccgtttgct tggccagttc
    12421 tttgacctcg ttggccacga cggcgaagcc tttcccagcc tcgccggcgc gcgcagcctc
    12481 gatggtggcg ttgagagcga gcaggttggt ctgctgtgcg atggaagtga tgactttgat
    12541 gacttcgccg atctcggcag aagactcgcc gagtttcgag atggtgttgt tagccgcctg
    12601 ggcggtctgc acggcgctgg aggcgacgct ggccgcctcg tgggcgttgg aagcgatgct
    12661 ctggatggta gtagtcattt ctcccgcgcc cgtcgagaca ctctgcagat tctggctgac
    12721 ctgctgcgta gcctgggaga cgaccccgac ctgtgccgat gtctcttccg agttggcgga
    12781 aatctgctgg ctggtcgccg agagttctgc gctggcgctg gccacgtgcc ccgaggtgtc
    12841 cgcaatggcc tgcaccacgg catgcaggtt gttcttcatg gtgttcatgg ccgtcatggc
    12901 atcgccgact tcgtccgcag agctgacatc caaatcgtcg agcgacaggt tcttggccgc
    12961 gatcgcctcg gcgaattccg cgcactcctt caccggacga atgaccagtt gccgcagcac
    13021 gtagtacatc accacgatgg ccgccagcac ggtgaagaag ctgaccagaa aggtcgccat
    13081 catgttcgag cgcgactcct gcaccagctt gtccgtcggc gcctccagca caaacgcggc
    13141 gcgcagctcg cccgccttca ttccttcctt ggggtagccg aagggatctt tctgcccggc
    13201 aggatcgcca tgacattgca ggcagtcctg ctcgacccgc accggctggg cgtatcgcat
    13261 gatttcccgg ccgtcgatct cggcgcggtc gtagaactcg gccagtgtgg agtctcgctc
    13321 gaaggcctcg agcgcccgcc gctcaaaatc cgttgcctgg ttcttgggat tgcgcgggtg
    13381 cagcgacggg gtgcggaact tcatcccctc cttgctggcg tattcctccg ccgtgcgcat
    13441 ggccaccacc accggcacct gttccaggtg cttgaagtcg cggtttggca ccatcaggtc
    13501 gatattgccg gcgaaccagt tgcgcgtcgc cgaagccatg cccgtaatct ggcgcagctt
    13561 gtcgcggaag gcatcctccg cctgccggtt ggcccggttc tgcatgatcc aaaagctaat
    13621 cccggaggtg atcgctagaa ttgcaacgat cgttcccagg agtttggccg acaatttcag
    13681 attgctgacc atatggtttg cctctatcgt ggttccttcg cgtgcattct ctgcttcaac
    13741 gtcctgcggc cgccgccatc gtcttgacgc gaccttgcgg agaaggcgga cggcctgcgc
    13801 ccatcgtcct gctgttgagc ttgaactggg cgaccaggtt g
//
LOCUS       DASPUL010000291         5568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_457933, whole genome shotgun sequence.
ACCESSION   DASPUL010000291 DASPUL010000000
VERSION     DASPUL010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457933"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VEJ00_07180"
     CDS             <1..1100
                     /locus_tag="VEJ00_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXX00979.1"
                     /translation="NLAQGFPDFPAPTEIKQAAQQAVAADVNQYAITWGAKSLRNAIA
                     RQTETSHGIQIDPEREITVCCGSTEAMISTLLAVCNTGDEVVIFEPFYENYGPDSVLS
                     GAKPKFVKLRPPKDENGEWTFDEKELRRAFDRHTKAIIVNTPNNPTGKVFTRAELELI
                     RDLCVEFDVLAITDEIYEHILYDGTRHISMASLDGMGERTVTINGMSKTYSVTGWRVG
                     WAIAPEKITNAIRKVHDFLTVGAPAPLQEAGAAALGLPTEYYAKLAEGYRVRRDHLIP
                     ALVEAGFKCFRPRGAYYVMTDVSAFGFPDDVSFARYLVQDIGVATVPGSSFYRDPRDG
                     ARQVRFAFCKKPETLDEAARRLAKLKATSRG"
     gene            1106..2065
                     /locus_tag="VEJ00_07185"
     CDS             1106..2065
                     /locus_tag="VEJ00_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00980.1"
                     /translation="MTLRTIAPRVGIFGWYLAWLLAARRFSLPPEVWYWLTFLFGACG
                     LIAGLVLFFRGFQLLQRKRWIEDTPVTKIAAAAVGSVKIFGKATGPYTLLSPLAGVDC
                     YYYRAVAWSGRDAEDEEQPQGRATEILFTPLFVEDETGCLMVDPRGASIELPAEYDEQ
                     ISSEAMAECSRRFLRRHGLSTAVGTTVSEYAIKPGDPILVLGTLGETRGLGSMADGDV
                     TADRTAYLSREAADLQRREQLEALGIPLSETREPAQNEDSHFDLRPRAVLQAGGDQPF
                     VLSREQPQRMIRNLARKSLLDIWGGPAIALLSLTLLLKWLGMW"
     gene            complement(2087..2599)
                     /locus_tag="VEJ00_07190"
     CDS             complement(2087..2599)
                     /locus_tag="VEJ00_07190"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HXX00981.1"
                     /translation="MKRHVHLLLGLLLVAAFPLRSAAKTNVDLKDGQGKPVGNIILWD
                     QGPGVGLELHLHDLTPGEHAIHFHQVPKCEGPDFKSAGGHFNPEGKKHGFDNPDGHHA
                     GDMKNFTVGADGKADLRLEDNDVTLKDGPHSLLSNGGSALVVHAKADDYKTDPSGNSG
                     DRVACGVITK"
     gene            2744..3031
                     /locus_tag="VEJ00_07195"
     CDS             2744..3031
                     /locus_tag="VEJ00_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00982.1"
                     /translation="MALNAVEQVSEQVPADDFEALEAKVYRTIEMYKAAREAQAAAER
                     DAQRLRQQLDEREAELATMRREAVHLRKEREAIRGRVEKMLAQIESIAEAS"
     gene            3073..3408
                     /locus_tag="VEJ00_07200"
     CDS             3073..3408
                     /locus_tag="VEJ00_07200"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HXX00983.1"
                     /translation="MATATKDHVVKDAQNAGVRVEIFDQAYNLRGSDPEYILKLADYV
                     DTKMRAVAEATNTIDTVRLAVLAALNIADEYHLLKKKLDSGATDYQKRAHLLADALDE
                     VLRDNRKAG"
     gene            3365..4381
                     /locus_tag="VEJ00_07205"
     CDS             3365..4381
                     /locus_tag="VEJ00_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXX00984.1"
                     /translation="MRSTKCCAIIARPGSLRAGTLLLAVALLGSLALSQETTLRSQAN
                     VVLIPTLVKDQQGGIFYGLKAEDFMIEDDGRELPARLDEAPEGQPISLVVAIQRGRRA
                     YYEFQRMQGLKTMLEPLFAQGTARVAIVEFDSQVELTRNFTNDASLISDDLTSLRPGD
                     NGTTILDAVNYSLGLLRQESEDRLRVLLLISETRDHGSHVKIDQTVTAVGQANAVMYA
                     LAFSPALSNILDTGRGTNKDEMHNGVNFLDLAYRTGQAMRKNAPQTLAAMTGGEYELF
                     ATRKKFDLRMNDFTNHLHGRYLLSIAPKDPRPGLHQIRVRLNTGRTGQVLARSSYWVD
                     GPNR"
     gene            4608..4799
                     /gene="ssrS"
                     /locus_tag="VEJ00_07210"
     ncRNA           4608..4799
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VEJ00_07210"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            4896..>5568
                     /gene="lgt"
                     /locus_tag="VEJ00_07215"
     CDS             4896..>5568
                     /gene="lgt"
                     /locus_tag="VEJ00_07215"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXX00985.1"
                     /translation="MYPQLFHIGRFFLPTYGFLVSLGVLIGLWISVRNSERLGIDGDK
                     AWNFGILVVLSGIIGAKVLYVINEWSTYKDHPGDIFSLATLQAGGVFSGGLLAAFVVA
                     AWYVWRHHLPPLGTCDAFSPGLALGHSIGRLGCFAAGCCYGKPTHHWWGVTFTNPLAN
                     AITGTPLGHPLEPTQLFESAIELANFIFLMWLLKRRKFEGQIFGAFMFIYGVARFFLE
                     FLRDDP"
BASE COUNT         1119 a         1633 c         1670 g         1146 t
ORIGIN      
        1 tcaacctggc ccagggattc cctgacttcc ccgccccgac ggagatcaaa caggctgcgc
       61 agcaagccgt cgctgccgat gtgaatcagt acgccatcac ctggggcgcg aagagtctgc
      121 gcaacgccat cgcgcggcaa acggagacgt cgcacggcat ccagatcgat cccgagaggg
      181 aaatcaccgt ctgttgcggc tcgacggagg ccatgatctc gacgctgctc gccgtgtgca
      241 acaccggcga cgaagtcgtg atcttcgagc ctttctacga gaattacggg cccgactcgg
      301 tgctcagcgg cgcaaagccg aaattcgtca agctgcgtcc tccgaaagac gaaaacggcg
      361 agtggacgtt tgacgagaaa gagctacgcc gcgccttcga ccggcacacg aaggccatca
      421 tcgtgaacac gcctaacaat cccactggca aggtgttcac gcgagccgag ctggagctga
      481 ttcgtgatct gtgcgtcgaa ttcgacgtgc tcgccatcac cgacgagatc tacgagcaca
      541 tcctctatga cggcacgcgg cacatttcca tggcgtctct ggacggcatg ggcgagcgca
      601 cagtcacgat caatggcatg tcgaagacct acagcgtaac gggatggcgc gtgggctggg
      661 ccattgcgcc cgagaagatc acaaacgcca ttcgcaaggt gcacgacttc ctgaccgtgg
      721 gagcgcccgc tcctctgcag gaagccggcg ccgccgctct cggcctgccc accgagtact
      781 atgcgaagct ggccgagggc taccgcgttc gccgcgatca tctgattccc gcactggtgg
      841 aagcggggtt caagtgcttt cgcccgcgag gcgcctacta cgtgatgacc gacgtctccg
      901 cgttcgggtt tcccgatgac gtttcattcg caagatatct ggtgcaggac atcggtgtgg
      961 cgaccgtgcc cgggtcgagc ttctaccgcg atccgcgcga cggggcacgc caggtacgtt
     1021 tcgccttctg caaaaagccg gaaactctgg acgaagcggc acgccggctg gcgaagctga
     1081 aggcgacgtc gcgcggctag tttccatgac tctgcggact atcgcgccac gagttgggat
     1141 cttcggatgg tacctggcgt ggttgctggc cgcgcgccgt ttctcccttc cgcctgaagt
     1201 ctggtattgg ctcacatttc ttttcggtgc ctgcggtctc atcgcaggcc tggttctctt
     1261 ttttcgcgga ttccagctcc tgcagcggaa gcgctggatc gaagacacac ccgttaccaa
     1321 gattgcggct gccgcagtgg gatctgtgaa gatcttcggc aaggcgaccg gaccctacac
     1381 gctgctttcg cctttggccg gcgtcgattg ctactactat cgggcggtcg cgtggagtgg
     1441 gcgggacgcg gaggacgaag aacaaccgca aggccgcgca accgagattc tctttacgcc
     1501 cctgtttgtg gaggatgaaa ccggatgcct catggtcgac ccgcgtggtg catcgattga
     1561 gttgcccgct gaatacgacg aacagatttc gagcgaggca atggccgagt gctcccgtcg
     1621 cttcctgcgc cggcatggac tttcgaccgc ggtcggcacg accgtcagcg aatacgcgat
     1681 caagcccggc gaccccatcc tggtcttggg cacgctggga gaaacccgcg gtttaggcag
     1741 catggccgat ggcgatgtga ccgctgaccg cactgcatac ttgagccgcg aagcggccga
     1801 cctgcagcgc cgggaacagc tcgaagccct gggtataccg ctttcggaaa cgagggaacc
     1861 cgctcagaac gaagattccc acttcgacct gcgtcctcgt gctgtgttgc aggccggcgg
     1921 cgatcagccg ttcgttctat cccgcgaaca gccgcagcgc atgattagga atctggcgcg
     1981 aaagtcgctt ctcgacatct ggggtggtcc agcgatcgcc ctgctcagcc tcacacttct
     2041 gctgaagtgg ctgggtatgt ggtaaatgcg ccagcccgac ggaaacctac ttggtgatta
     2101 ctccgcaggc cacgcggtct cctgaattgc cagacgggtc ggtcttgtag tcgtccgcct
     2161 tggcatgaac cacgagtgcg ctgcctccat tgctgaggag ggagtggggg ccgtccttta
     2221 gagtaacgtc gttgtcctcc agccgcagat cggccttgcc atcggcacca accgtgaaat
     2281 ttttcatgtc gccagcatga tgaccgtcgg ggttgtcgaa gccgtgcttc ttgccctcgg
     2341 gattgaagtg tccgcccgcc gacttgaagt cgggaccttc gcatttcggc acctgatgga
     2401 agtggatggc atgctccccc ggtgtgaggt cgtggaggtg gagttcgaga ccgacgcccg
     2461 gtccttgatc ccagaggatg atattgccaa cgggcttacc ctgaccgtct ttcagatcca
     2521 cattggtctt ggccgcgctc cgcaacggga aagcggcgac cagcaatagt ccgagcagca
     2581 gatgaacatg gcgtttcata agtcgaccct ccgcgagcag gaattatagc caatccggcc
     2641 cgaacagggg gctgaaaacg gccaggcgat gtctgagttc ttcacaccga ggtggagtat
     2701 actgacggga aatcccgaac tcgcagatga ggaaaaggta atcatggcgt tgaatgcggt
     2761 cgagcaggta tcagagcagg ttccggccga cgattttgag gcgctggaag cgaaggtcta
     2821 tcgcaccata gaaatgtaca aagccgctcg tgaggcgcaa gctgcggcgg agcgcgatgc
     2881 gcagcgtttg cgccagcaac tcgacgagcg tgaagccgaa ctggcaacca tgcggcgcga
     2941 agccgtacat ctcaggaaag agcgcgaggc tatccgcggg cgggttgaga aaatgctggc
     3001 gcagatcgag tcgattgccg aagccagcta ggcgcccgga gagacgcggc cagcgtgacg
     3061 gcggaggagg ttatggctac tgcgaccaag gatcatgtgg tgaaagacgc gcagaacgcc
     3121 ggcgtgcgcg tggagatttt tgatcaggcc tacaatctgc ggggctcgga tccggagtac
     3181 attctgaagc tggcagacta cgtcgacacc aagatgcgcg ccgtcgccga ggccaccaac
     3241 accatcgaca cggttcgtct cgctgtcctg gcggccctta acatcgccga cgagtaccat
     3301 ctgctcaaga aaaagctcga ctccggggca accgactatc agaagcgagc gcatctacta
     3361 gcagatgcgc tcgacgaagt gctgcgcgat aatcgcaagg ccgggtagtc tgcgcgccgg
     3421 aaccttgctt ctggcagttg cattactcgg aagcttagcc ctctctcagg aaaccaccct
     3481 tcgttctcaa gccaacgtcg ttttaatccc cactctggtc aaagatcagc agggcggaat
     3541 tttctacgga ctgaaggccg aggatttcat gattgaggat gacggccggg aactacccgc
     3601 gcgactggac gaagcgccgg aagggcagcc gatctcgctg gtagttgcga tccagcgcgg
     3661 gcgccgggcc tactacgagt tccagcggat gcaaggtctg aagacgatgc tcgagcctct
     3721 attcgcccag ggaacggctc gggtcgccat cgtggagttc gacagccagg tggaactcac
     3781 gcggaatttt acgaacgacg cgagcctgat ttccgatgac cttacaagcc tgcggccggg
     3841 agacaacggg acaacaattc tggatgccgt gaactactcc ctcggcttgc tccggcagga
     3901 gagtgaagac cgcttgcgag tgctgctgct gatcagcgaa acacgcgacc acggcagcca
     3961 cgttaagatc gatcagacgg tgactgctgt cggtcaggcc aatgccgtga tgtatgccct
     4021 cgcgttctcg cctgcacttt ccaacattct cgacaccgga aggggaacga acaaggatga
     4081 gatgcataac ggagtcaact ttcttgatct ggcgtatcga acggggcagg ccatgcgaaa
     4141 gaacgctccc caaacactcg cggccatgac cggaggcgag tacgaactgt tcgccacgcg
     4201 caagaagttc gatctgcgca tgaatgactt caccaatcac ctgcacggcc gttacttgtt
     4261 gagtattgcg ccgaaggacc cgcgtcccgg gctgcaccag attcgggttc gtttgaatac
     4321 cgggaggact ggtcaagtgc tggcacggag tagttactgg gttgatgggc cgaatcgcta
     4381 ggaatccgct gtgttcgccg cgattgcgta caccggacgg ctgcacttct ggatcttgtg
     4441 gtgccggcct tgccgtggtg ccgatgaaaa cgagatagaa aatccggagt cttcaatgca
     4501 agcccttctt gtgaacgcag cattactttg gttgcaagcg ggctgccaac tttttccgct
     4561 cttccccttg ccaatccgcc agcttttccc tagcatccaa attgaaagcc ggcctgcaag
     4621 gttggtgatg acgggtaacc gatttttgaa ccaatgctga atgaacggga gctcgactcg
     4681 aactaagatc cggtgagcga cgctccgcca tgcggagatg cctaaagggt ctcggcgagc
     4741 gcccaacgtc taccgacggg ttcattctcg gccccgccac acggctccgt gggtcggctt
     4801 gaattttgcc tttggctctt agccaacggc caaatgcatc tttcctgcta gactgatccc
     4861 gtagcaaatc ccgcgattcc tgcatctaat tcagcgtgta cccgcagctc tttcatatcg
     4921 gacgcttctt cctgccgacc tacggattcc tcgtgtctct gggcgttctg atcggtctct
     4981 ggatcagtgt tcgtaactcg gagcgcctcg gcatcgacgg cgataaggcg tggaacttcg
     5041 gcatcctcgt tgttttgagc ggcatcatcg gcgcaaaggt gctttacgtt attaatgagt
     5101 ggagcaccta caaggatcat cccggtgata tcttcagcct cgcgaccttg caggcaggcg
     5161 gagtattttc cggcggctta ttggccgcgt ttgtggtcgc cgcatggtat gtgtggaggc
     5221 atcatttgcc gcctctcggc acgtgcgatg ccttctcgcc cggtcttgcc ctgggtcact
     5281 cgatcggccg cctcggctgc ttcgcggctg gatgctgtta tggaaagccg acacatcact
     5341 ggtggggcgt cacgttcaca aatcccctcg ccaacgcaat tacgggtact cccctgggcc
     5401 atcccctgga accgacgcag ttgtttgaat ccgcgatcga actggcgaat ttcatcttcc
     5461 tcatgtggct gttgaagcgg cgcaagttcg agggccagat ctttggcgcc ttcatgttta
     5521 tttatggcgt cgcgagattt ttccttgaat ttctgcgcga cgaccctg
//
LOCUS       DASPUL010000292         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1416407, whole genome shotgun sequence.
ACCESSION   DASPUL010000292 DASPUL010000000
VERSION     DASPUL010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416407"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1291)
                     /locus_tag="VEJ00_07220"
     CDS             complement(185..1291)
                     /locus_tag="VEJ00_07220"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF002898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXX00986.1"
                     /translation="MNQKKDIPKSSGKAKKKRIAVIPGDGIGQEVIPQAVKVIQASGA
                     GVEMTEFDWGADRYLRDGTIVPPDGFAILARDFDAILVGAFGDPRVKTNIHAKEILLG
                     MRFKMDLYANVRPVRLLDAALCPLKGVEPKDVDFVVVRENTEGVYTDAGGVFKQGTSD
                     EIATQEDINTRKGVERIIRYAFDYCQHHQKLDGSPRSKVLMCDKSNAMTHAGGLWQRV
                     FKEVSGEYPQITVQHMYVDALCMQMVRDPRQFDVIVTNNMFGDIITDLAAGLQGGLGM
                     AASGNIHPKRTSMFEPVHGSAPPIAGKNIANPFGAILTAAMMLRSLAMAKEADRIEAA
                     VMEAVRQKKTTQDIGGALGTREAGEWVAEKVGRG"
     gene            1333..>2240
                     /locus_tag="VEJ00_07225"
     CDS             1333..>2240
                     /locus_tag="VEJ00_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00987.1"
                     /translation="MGLTALQTQSEIDGQGLGATRTTEYRGAVTATRFTDPNAELAAL
                     RGTCGVYDLGYRAKISLTGSDRVRWLNGMVTNNIRDLAAGHGVYAFLLNPQGHILGDL
                     YAYNRGESGVSSSIMVDTDRTQLERVLATFDHYIIMDDVEVTNLSDQMTALGVAGPGV
                     RNTLLAAGIAVPGTEPLELFTPRCTCACECLQCTMVRGESASVESYEIWIAPAGVGKL
                     WRALVAAGATPVGWEALELQRILSGVPRYGVDIRERDLPQETEQMQALNFNKGCYIGQ
                     EIVERIRSRGAVHRKFTGFLADSAIE"
BASE COUNT          410 a          674 c          695 g          461 t
ORIGIN      
        1 agtcagggag ttgccgtgga agagcggtcc ttcagggccg cgtaagagcc ttgaggcagc
       61 gccctttagg gctggtggtc gttttcgatc gggtcatgaa aggcgaccac gagggctaaa
      121 gcccccggcc ggtgtgaacg cttaacgcgg cgctgaagcg ccgctcttcc acgatatctc
      181 cgggctatcc gcgaccgacc ttctccgcca cccactcccc tgcttcccgg gtccccagtg
      241 cgccgccgat atcctgcgtg gtctttttct ggcgcacggc ttccatcacg gcagcttcga
      301 tcctgtccgc ttcctttgcc atcgccaggg accgcagcat catcgctgcc gtcagaatcg
      361 cgccgaaggg atttgcaata ttcttgccgg caatcggcgg agcggaccca tgcaccggct
      421 cgaacatcga cgttcgtttt ggatgaatgt tgccgctggc ggccatgccc agccctccct
      481 gcaggccggc ggcgaggtcg gtgatgatgt cgccgaacat gttattcgtg actatcacgt
      541 cgaattggcg cgggtcgcgc accatctgca tgcagagcgc gtcgacgtac atgtgctgca
      601 ccgtgatctg cggatactcg ccgctgactt ccttgaacac gcgctgccac aagcccccgg
      661 cgtgggtcat ggcgttcgac ttgtcgcaca tcaggacctt gctgcgcggc gaaccatcga
      721 gtttctggtg gtgctggcaa tagtcaaagg catagcggat gatccgctcc actcccttgc
      781 gcgtgttgat gtcttcctgc gtggcgatct cgtcggacgt gccctgcttg aaaacgccgc
      841 ccgcgtcggt gtaaacgcct tcggtgttct cgcggaccac gacgaagtcc acgtctttcg
      901 gctcgactcc tttcagcggg cagagcgcgg catcgagcag gcgcacgggg cgcacgttgg
      961 cgtagagatc catcttgaag cgcatcccca gcagaatttc tttggcatgg atgttggtct
     1021 tcacccgcgg atcgccgaaa gcgcccacga ggatggcgtc gaagtcgcgg gcgaggatgg
     1081 caaagccgtc gggaggaaca atcgttccgt cgcgcaggta gcggtcggcg ccccagtcga
     1141 actcagtcat ctcaacaccg gcaccggagg cctgaatgac cttgaccgcc tgcgggatga
     1201 cctcctggcc gatgccgtct ccgggaatga cggcgatacg tttcttcttc gctttgcctg
     1261 atgattttgg tatgtccttt ttctggttca cggcaggaac gttaacacaa tcgttataat
     1321 cgaactacca gcatggggct cactgcactc caaactcaga gcgagatcga cggccaaggc
     1381 cttggcgcaa cgcgtacaac cgagtaccgc ggcgccgtta cggccacgcg gttcaccgat
     1441 ccgaacgcgg aattggccgc cctgcgcggg acttgcggcg tttacgatct cggatatcgc
     1501 gcgaaaatct cgctgacggg ctcagatcgc gtgcgctggc tgaacggaat ggtcaccaac
     1561 aacatccgcg atcttgccgc agggcatggg gtttatgcgt tcctgctcaa tccgcaaggg
     1621 cacattttgg gcgacttgta cgcctacaac cggggcgaat cgggcgtttc tagttcaatc
     1681 atggtcgaca ccgatcgcac tcaactggaa agggtgctgg cgaccttcga tcactacatc
     1741 atcatggatg atgtggaggt cacgaatctc agcgaccaga tgacggcgct cggcgttgcg
     1801 ggccctgggg tacgtaatac actgctggct gcagggattg cggtcccggg gacagagcca
     1861 ctcgagttat tcactccacg ctgcacttgc gcgtgcgaat gcctgcagtg caccatggtt
     1921 cgcggagaaa gcgcgagcgt ggaatcgtac gagatttgga tcgctccggc gggcgtggga
     1981 aagctctggc gagcgttggt cgcggctggc gccacgccag taggttggga ggcgctggag
     2041 ttacagcgca ttctgtctgg cgttccgcgc tacggcgtcg atatccgcga gcgcgacctg
     2101 ccccaggaaa ccgagcagat gcaggccctg aacttcaaca agggatgcta catcgggcag
     2161 gaaatcgtgg agcggattcg ctcgcggggg gcggttcacc gcaagttcac gggattcctg
     2221 gccgacagtg cgatcgagat
//
LOCUS       DASPUL010000293         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_878788, whole genome shotgun sequence.
ACCESSION   DASPUL010000293 DASPUL010000000
VERSION     DASPUL010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878788"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VEJ00_07230"
     CDS             complement(<1..659)
                     /locus_tag="VEJ00_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXX00988.1"
                     /translation="MKLLIVVQHRLDLWNVPKWFSDRLGQQFPQLQIVLRNHYAGIEQ
                     LLHDAEIIFTLSLRPEQFVLAQRLRWIHTPSAAVHQLMFPELVNSEVMVTNSGEVHGP
                     VVAEHVMALVFALAKKVPQAVLLQQKRVWGQQEVWNAHPRELAGATLGLIGVGSIGRR
                     VARFASALGMRVIAVREHVEKGTPEGVEAVFAPSALNHFLRQCDYVVLAAPLTEATEG
                     L"
     gene            854..1801
                     /locus_tag="VEJ00_07235"
     CDS             854..1801
                     /locus_tag="VEJ00_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00989.1"
                     /translation="MKRIGSVLLAITVALPLSAQVVAPPPPQSARQALIEMFFGTRED
                     DFAKHLPEAARQTLIRKGENPETSVALRISAIGRQVVAQGQHLETFDTGSTLLVTEPA
                     KGEKIEVMVESDSVHGDQEEIELSVHYYKDGQLQPLPVVPLFTFAFQQQREIWRLLEV
                     TAAAHVPLTDPDYLKGLRRQQDESYESSAQMRMNAIMAAETRYAAKYPGVGYSCALTS
                     LFSREVAGTPGEGPFLFDPGQGNDEWNGYRFTLSGCEGSPVLKFRVLAAPIDPDSEAK
                     TFCADESGSLKFVSGGKSSACFTRGEPLATGSDAGGFTE"
     gene            complement(1821..2645)
                     /gene="proC"
                     /locus_tag="VEJ00_07240"
     CDS             complement(1821..2645)
                     /gene="proC"
                     /locus_tag="VEJ00_07240"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HXX00990.1"
                     /translation="MANDLAGKKVAFLGAGKMGGIMLQALLKKGLLSPKSSRATVAHP
                     ERAKALATKLKVKVGTDNVEAVKGADIIVIAVKPQVVEDVVREISPHITPKQKIVSVA
                     ASVPTAMIERNLASNVPVIRAMPNTPCLLGAGMTAIAKGKHASAADVALTCSMFDAVG
                     RTVVVDEKHMDAVTALSASGPAYIYIILESLAEAGVKVGLPRDVATMLAAQTTLGAAR
                     VVLETGDHPALLKDAVTTPAGCTIDAIMELEEGKLRVTLIKAVVKATHRAKELAFG"
     gene            complement(2672..>3149)
                     /locus_tag="VEJ00_07245"
     CDS             complement(2672..>3149)
                     /locus_tag="VEJ00_07245"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXX00991.1"
                     /translation="GADESGEIGELHDPETHLIPIALRAAAGIGRELQVFGSDYPTPD
                     GTCIRDYIHVNDLADAHVKALEQLATGHESFAVNLGTGKGSSVKEVISAVGEVTGKRV
                     PHRMAPRRTGDPAALVANPARAQRLLQWKATRGLRDMVATAWNWMERQPRATSSKN"
BASE COUNT          589 a         1007 c          927 g          626 t
ORIGIN      
        1 atcaagccct ctgtcgcttc ggtcagggga gcggccaaca cgacgtagtc gcactgccgc
       61 aggaagtgat tcagcgctga cggagcgaag acggcctcca caccttcggg agttcccttt
      121 tccacgtgct cccggacggc gatcacacgc atgcccaggg ccgaggcgaa cctggcgacg
      181 cgtctcccga tactgcccac gccgatcaat cccaaagttg cgcctgcgag ttcgcgcgga
      241 tgcgcgttcc agacctcctg ctgcccccac accctcttct gctgcagcag gaccgcctgc
      301 ggaaccttct tggccagcgc gaaaaccagc gccatcacgt gttcggcaac gaccgggccg
      361 tgcacttctc cagagtttgt gaccatcacc tcgctgttca ccagttcggg gaacatcaat
      421 tgatgcacag ccgcgctcgg cgtgtgaatc cagcgcaagc gttgcgccag gacaaattgt
      481 tcaggccgca aggagagcgt gaagatgatt tctgcgtcat gcaggagttg ctcgatcccg
      541 gcgtaatgat tgcgcaggac aatctgcagt tgcgggaact gttgcccgag ccggtcgcta
      601 aaccacttcg gcacgttcca gaggtcaagg cggtgctgga ccacgatcag cagcttcatg
      661 gtgcgcaagt ccggatcccg gaatttaaca tagaggcagg aggcaggagg catccttaat
      721 tcgctggcaa ccatccagca agatattcga taccacacgt tcctgaaaga acaggaacct
      781 tgatgcgctg cccggagtct tagatcgcag acattgggta ccgagcgcgc cgccgctcgc
      841 gtggagaagg catatgaagc gaatcggatc tgttcttctg gcaatcacag tcgctttgcc
      901 gctttccgcg caggttgtgg ccccgccgcc tccgcaatcg gcccggcagg cgttgattga
      961 gatgtttttt ggcacgcggg aggatgactt cgcaaaacat cttcccgagg ctgctcgcca
     1021 gactctgatc cgcaaaggag aaaatcccga gacgtcggtg gccttgcgca tctccgccat
     1081 cggccgccag gtcgtggcac agggccagca cctggaaacc ttcgataccg gctcgacgct
     1141 gctcgtcacc gagcccgcca aaggggaaaa aatcgaagtg atggtcgaga gcgacagcgt
     1201 gcatggcgac caggaggaga ttgagctttc cgtccactat tacaaagacg gacagttgca
     1261 gcccttgcca gtagtgcccc tatttacctt cgcctttcag cagcaaaggg agatctggcg
     1321 gcttctcgaa gtgaccgcag cggcgcacgt tccgctgacc gatccggact atctgaaggg
     1381 cctgcgccgg cagcaggacg aatcgtacga gtcctcggcg cagatgcgaa tgaacgcaat
     1441 tatggcggca gaaacccgct acgccgcaaa gtatcccggc gtggggtata gctgcgctct
     1501 caccagcctg ttttccaggg aggtagcggg cactcccgga gaaggcccgt tcctcttcga
     1561 tcccggtcag gggaacgacg agtggaacgg atatcgcttc actctgtcgg gctgcgaagg
     1621 ctcgccggtc ctgaaattcc gcgttctcgc cgcccccatc gatcctgatt ccgaagccaa
     1681 aaccttctgc gcggatgaat ctggaagcct caagttcgtg agcggaggaa agagctcggc
     1741 gtgcttcacc cggggcgagc ccttggcgac cggcagcgac gccggcggat tcaccgagta
     1801 gcggcgctgc aatcgcggcg ctatccgaaa gccagttcct tcgcccggtg cgtcgccttc
     1861 accaccgctt tgatcaaagt gacgcgcaac ttcccttctt ccagctccat gatggcgtca
     1921 atggtacagc cggcaggcgt ggtaacggcg tctttgagca gcgccggatg gtcgccggtt
     1981 tccagcacga cccgggcggc accgagcgtg gtctgcgccg ccagcatggt cgcgacgtcg
     2041 cgcggcagtc ccaccttcac gccagcttca gccagcgatt cgaggatgat gtagatatat
     2101 gccgggccgc tggcggagag agcggtgacc gcatccatgt gcttctcgtc gaccaccacg
     2161 gttcggccga ccgcgtcgaa catgctgcag gtcagggcga catcggcggc gctggcgtgc
     2221 ttgcccttgg caatcgccgt catgcccgct cccagcaggc acggcgtatt gggcatggcg
     2281 cgaatcacgg gcacgttgga ggcgagattc ctttcgatca tcgcagtcgg gaccgacgcc
     2341 gcgacggaga caattttctg tttgggcgtg atgtgcgggc tgatttcgcg caccacatct
     2401 tccacaacct gaggtttcac cgcgatcaca atgatgtcgg cgcccttgac cgcctcgacg
     2461 ttgtcggtac cgaccttcac tttgagtttt gtcgccagtg cttttgcccg ctccggatgc
     2521 gccaccgtgg cccgcgagga ctttggcgag agcaggccct ttttcagcag cgcctgcagc
     2581 atgatgccgc ccatcttccc cgcacccaga aatgcaacct tcttccctgc taaatcattg
     2641 gccatgattt cctctgtctg ttacgcgtat gtcagttttt gctggaagtg gctcgaggct
     2701 gccgctccat ccagttccag gcggtcgcca ccatgtcgcg caatcctcgc gtagccttcc
     2761 actgcagcag ccgctgcgct ctggcaggat tcgccaccag tgcggccgga tcgccggtgc
     2821 gccgcggagc cattctgtgc ggcacccgct ttccggtcac ctctcccacc gcagaaatca
     2881 cttccttgac cgaggatccc ttgcctgtgc caaggttgac tgcgaagctc tcatgccccg
     2941 tcgccagctg ctccagcgct ttgacgtgcg cgtctgccag atcgttcacg tggatgtagt
     3001 cgcgaatgca ggttccgtcg ggagtgggat agtccgagcc gaacacctgc agctcgcgac
     3061 cgatcccggc ggcggccctc agggcgatcg gaatcagatg agtttccgga tcgtgcagtt
     3121 ctccgatctc gccactttca tccgcgccg
//
LOCUS       DASPUL010000294         4189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1416796, whole genome shotgun sequence.
ACCESSION   DASPUL010000294 DASPUL010000000
VERSION     DASPUL010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416796"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VEJ00_07250"
     CDS             complement(<1..324)
                     /locus_tag="VEJ00_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00992.1"
                     /translation="MARTISKPRKAAANENPARSGSSRRSTGRATLAASTKHFLSDKV
                     AHFTESVIREMTRQAMLYEAVNLAQGFPDFPAPTEIKQAAQQAVAADVNQYAITWGAK
                     SLRNAI"
     gene            466..939
                     /locus_tag="VEJ00_07255"
     CDS             466..939
                     /locus_tag="VEJ00_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXX00993.1"
                     /translation="MVAPHRGNTGYSTYFITAATFQKQNLFQSDRLTLPFIDTLLHYR
                     VQRSYPLHKFVIMPNHFHLLVTPIQSLERALQLIKGGFSYRAKKELGFLGEIWQSSYY
                     DRRVRNAEEYVNFRYYIRHNPVKRGLAQTPEEYSYSSACPDFVRDEVPKKLRLIA"
     gene            complement(1044..2882)
                     /gene="aspS"
                     /locus_tag="VEJ00_07260"
     CDS             complement(1044..2882)
                     /gene="aspS"
                     /locus_tag="VEJ00_07260"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXX00994.1"
                     /translation="MLDFLGDLRRTHMCGALRPSDAGKKAVLMGWVNRRRDLGSIIFI
                     DLRDRSGVAQVVFNRERNAAAHDKAEVLRNEYVIAAIGTVKLRDADTVNKNIPTGEVE
                     LVVDEIRILNESKQPPFLPSDTALTNEETRLKYRYLDLRRDAMQFNIETRHKVTKAIR
                     DYLSAQGFFEIETPFMTRSTPEGARDYLVPSRVQPGAFYALPQSPQMFKQILMIAGFD
                     KYFQIVRCFRDEDFRADRQAEFTQIDLEMSFPQAERVWEVVEGFLVAAFRAAGYEIKP
                     PFPRMDYDEAIRLYGIDKPDLRLPPFTDVRDCFTAENLHELAINANLPVIAIRTPKVG
                     ELSRKERDDIKPMFHSKGGAKIFEDFKRIGNKYPDAASAIAKKTAMESDDLIVLVAGS
                     AQAGPQTPMPAHRKVTPAELAIYASAGLLRLALAQKYAERHKCFQRGDFRFLWVTNFP
                     MFEWDENEKQWMAAHHPFTSPHEEDMGLLGAGVDSVNDPQSPLSAVRALAYDVVLNGT
                     ELGSGSIRIHRQDIQSKIFRALGMTDDEAKARFGFFLEALEYGTPPHGGIALGLDRIV
                     MILAGAESLREVIPFPKTARAVDLMVDAPTPVSERQLRELGIQIKK"
     gene            3209..>4189
                     /locus_tag="VEJ00_07265"
     CDS             3209..>4189
                     /locus_tag="VEJ00_07265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXX00995.1"
                     /translation="MATSEYTISTLPANVEAERSILGAILLDNFAYNQAAEHLRIEDF
                     SLDSHRRIYSRMVDLAESSRPIDMITLIEELDRHKDLQAIGDVAYVSSLIEGVPERPS
                     IEHYVKIVRDKALLRGLISAANTAIARASDQSDLAEEVLSDAEAAIFQLSEKRIGRGF
                     MGVQEIVRESFGSVDALLQRGQRITGLATHYSDLDEMTSGLQRADLAIIAARPSMGKT
                     AFAMNIAENAAIEDKQVVGVFSLEMSREALLLRLLCSQARVDAHKMRTGSLWQDDTKK
                     VVRAMEQLAHAPIYIDDTPGITVSEMRAKARRLKQAQGRLDLLIVDYLQLM"
BASE COUNT          867 a         1230 c         1235 g          857 t
ORIGIN      
        1 gatggcgttg cgcagactct tcgcgcccca ggtgatggcg tactgattca catcggcagc
       61 gacggcttgc tgcgcagcct gtttgatctc cgtcggggcg gggaagtcag ggaatccctg
      121 ggccaggttg acggcttcat aaagcattgc ctgtcgggtc atctcgcgga taaccgattc
      181 agtgaagtgc gcgaccttgt cgctgaggaa gtgcttggtt gaggcagcca gcgttgccct
      241 ccctgtgctt cgcctcgaag atccgctacg ggcgggattc tcgttggcgg cggcttttcg
      301 gggtttggaa atcgtccttg ccatgagagg cagactagca cagcgagatg tggcatggtt
      361 ccaaagcggg acactacact gaaaaacatg acttgaggtg gcgcagcgct tccagcgctg
      421 cgataaagtg gccaggtgtg tgcggcttca gccgcggagg tacttatggt agcaccacac
      481 cggggcaaca cgggatacag cacctacttc ataaccgccg ctacattcca gaagcagaat
      541 ttgtttcaat cagatcgact cacactgcca ttcatcgaca cactgctgca ttaccgtgtt
      601 cagaggagct atcctctgca taaatttgtc ataatgccaa atcatttcca cctcctggtg
      661 actcctatac aaagtctgga gagggctttg cagctgatca aaggtgggtt ctcctatcga
      721 gcgaaaaagg aactgggatt cttgggagag atctggcagt ccagctacta cgatcgtcgt
      781 gtgcggaatg cggaagagta tgtcaatttc aggtattaca tccgacacaa tcccgtgaag
      841 cgtggccttg cgcagactcc ggaagaatat tcgtacagtt cagcctgtcc tgactttgtc
      901 cgggatgagg ttccaaaaaa actgagactg attgcgtgac gaatggggca cctcagcggc
      961 taaagccgcc tcttcagtgc tatctcgatc gcagcgctgg aagcgctgcg ccaccccaaa
     1021 gcgggatttc ttgtgcgaca tcgttacttc ttgatctgga tccccaactc ccgcaactga
     1081 cgctcgctga ccggggtcgg tgcatcgacc atcaaatcga cggcgcgggc agtcttgggg
     1141 aacggaatca cttcacgcag gctctcggca ccggccagga tcatcacaat gcggtcgagg
     1201 ccgagtgcga tgccaccgtg cggaggcgtc ccatattcga gcgcttccag gaaaaatcca
     1261 aaccgcgctt tcgcttcgtc gtcggtcatg cccagcgcgc ggaagatttt gctctgaata
     1321 tcctggcggt gaatacggat cgagcctgag cccaactcgg tgccattgag cacgacgtcg
     1381 taggcgagcg cgcgcaccgc actcagcggc gactgcggat cgttcaccga gtcgacgccc
     1441 gcgcccagca gacccatgtc ttcttcatgt ggcgaggtga aaggatgatg cgcggccatc
     1501 cactgctttt cgttctcatc ccactcgaac attgggaagt tcgtgaccca cagaaagcgg
     1561 aagtctccgc gttgaaaaca tttgtgccgc tcggcgtatt tctgcgccaa cgccagccgc
     1621 aacaagccgg ccgatgcata aatcgccaac tccgcaggcg tgaccttgcg atgggccggc
     1681 atcggggtct ggggtccggc ttgcgccgac cccgccacaa gcacgatcag atcatccgac
     1741 tccatcgcgg ttttcttcgc aatcgcagac gcggcgtccg gatatttgtt cccgatgcgt
     1801 ttgaagtctt cgaagatctt cgcgccgcct ttggagtgga acatcggctt gatgtcgtcg
     1861 cgctccttgc gcgaaagctc gcccactttg ggtgtgcgaa tggcgatcac gggcagattc
     1921 gcgttgatcg cgagctcgtg cagattttca gccgtgaagc agtcgcgcac atcagtaaat
     1981 ggcggcagcc gcaggtcggg cttgtcgatt ccgtagagac ggatcgcctc gtcgtagtcc
     2041 atgcgcggga acggcggctt gatctcatat ccggcagcgc ggaacgcggc cacgagaaat
     2101 ccttccacca cttcccacac tcgttcggct tgcgggaacg acatttccag atcgatctgg
     2161 gtgaactcgg cctggcggtc ggcacggaaa tcctcgtcac ggaagcagcg cacaatctga
     2221 aaatacttgt cgaaacccgc gatcatgaga atctgcttga acatctgcgg ggactgcggc
     2281 agggcgtaga acgctcccgg ctgcacgcgg ctggggacga gataatcgcg cgcgccttca
     2341 ggcgtggagc gtgtcataaa cggcgtctcg atctcgaaga atccctgcgc tgaaaggtag
     2401 tctcgaatcg ccttcgtgac tttgtgccgc gtctcgatat tgaactgcat ggcatcgcgc
     2461 cgcagatcga ggtagcggta cttcagccgt gtctcttcgt tagtcagcgc cgtatcggaa
     2521 ggcaggaatg gcggctgctt cgactcattc agaatgcgaa tctcgtcaac gaccagctcg
     2581 acctcccccg tcgggatgtt cttgttgacg gtgtcggcgt cgcgcagttt gacggtgccg
     2641 atggccgcga tgacgtactc gttgcgcagg acttcggctt tgtcgtgggc cgcagcgttc
     2701 cgctcgcggt tgaacacgac ttgcgccacg ccggaacggt cgcgcagatc gatgaagatg
     2761 atgctgccga gatcacgacg gcggttcacc catcccatga gcacggcttt cttgccggcg
     2821 tcggaaggac gcaaggcgcc acacatgtgc gtgcgtcgta aatcgcctaa gaagtcgagc
     2881 aagcggaatc cttacagtag gaatgcggca caaggccgaa ccttggatta tagacgctcg
     2941 caagtgaacc caaccagagc cgagctgccg tagaagcggc tggtttttcc gaccgcgtcc
     3001 acaggaatag gaaacgattc acaggttctt cacggtttcg ctgcgcattc cgagaaagac
     3061 tcttccacaa tcgccttaaa ctctctccct gacctgctcc gggaacgaat gagcaacgtg
     3121 cgaacccgac tcggagcttg cgtgagacta cggtttcgct ttatattcgc tcccaggcac
     3181 atcggctgca ggctagcaac caagacccat ggcgacttcc gaatacacga tcagcacgct
     3241 tccggcgaat gtcgaagccg agcgctccat tctgggcgcg atcctgctcg acaacttcgc
     3301 ctacaaccag gccgccgaac accttcggat cgaagacttc tcgctcgact cgcaccggcg
     3361 gatttattcc cgcatggtcg acctggccga gtcgtcgcgg ccgatcgaca tgatcacgct
     3421 gatcgaggag ctcgaccgcc acaaggatct tcaggccatc ggcgacgttg cctatgtttc
     3481 gagcctgatc gagggtgtac ccgagcggcc aagcatcgag cactatgtca agatcgtgcg
     3541 tgacaaggca ctgctgcgcg gattgatctc ggccgccaac accgcgattg ctcgcgccag
     3601 cgatcaatcg gacctggccg aggaagtact gagcgacgcc gaagctgcga tcttccagct
     3661 ctcggaaaag cgcatcgggc gcggtttcat gggcgtgcag gagatcgtcc gcgagtcctt
     3721 cggctcggtg gatgccctgc tccagcgcgg ccagcgcatc actggcctgg ctacgcacta
     3781 cagcgatctg gacgaaatga ccagcggatt gcagcgcgcc gatctggcca tcattgcggc
     3841 gcgaccgtcg atgggcaaga cggccttcgc catgaacatc gccgagaatg ccgccatcga
     3901 ggataagcag gtggtgggag tcttctcact agaaatgtcg cgcgaggcgc tgctgctgcg
     3961 gctgctgtgc tcgcaggcgc gcgtggacgc ccacaagatg cgcaccggat cactctggca
     4021 agacgacacg aagaaagtcg ttcgcgcgat ggagcagctg gcacacgcgc ctatttacat
     4081 cgacgatacc ccgggaatca ccgtcagcga gatgcgggcc aaggcgcggc ggctgaagca
     4141 ggcgcaaggc aggctcgatc tgctgatcgt cgattacctg cagttgatg
//
LOCUS       DASPUL010000295         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_324363, whole genome shotgun sequence.
ACCESSION   DASPUL010000295 DASPUL010000000
VERSION     DASPUL010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324363"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VEJ00_07270"
     CDS             complement(<1..379)
                     /locus_tag="VEJ00_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00996.1"
                     /translation="MRSLFRSVTPEVAPTVEPVPTVFPKIAGADIAAVFAGKRVAGDF
                     YDSVRVSPERVLFGLLDVAGRRDDTRSILSAAQGIFRKLGCELFSKPDINESDAMTEL
                     CLRINRGLIEASAGVRSCPAFIGC"
     gene            complement(433..1860)
                     /locus_tag="VEJ00_07275"
     CDS             complement(433..1860)
                     /locus_tag="VEJ00_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX00997.1"
                     /translation="MRVRVTFLLILVVWLVLSLYGARADTIHLKNGRSIVADHVRENG
                     AHYEYDVGDDSYAIPKSLVDHVDAGGMPIHSAAGTAKVADLPAFTPADSLANEGDLFS
                     KIIKEGRVDPDVLAALEAKGNAELSATASFIAGKFEFEHGNIAQARSYFESALRYQPE
                     NSTILIYYSALLVRTGNAAEALPYAQRAVRSSPSSPDAYTMLGYAQFASDRTKEAVAS
                     WKRSLELRPDAAVQQFLAKAQREDAVESDFSQHESSHFVLHYEGKQVSESFRSQLVAA
                     LESDYDDLSRDLGNPPRDSILVTLYTEQAFFDVTHAPTWSGAINDGKLRIPVSGLSTI
                     TPELARVLKHELAHSFINQLSGGRCPHWLNEGIAQLVEPKNLGGDGHQLALLFKAQRN
                     IPLNVLEGSFMNFSGVEAYVAYAESLAAVSYINDSYGMSDIQRILQRISQGSSTEAAL
                     RATIHSDYGQLESDLAKYLADRYGS"
     gene            1844..>2740
                     /locus_tag="VEJ00_07280"
     CDS             1844..>2740
                     /locus_tag="VEJ00_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00998.1"
                     /translation="MTRTRIQAHSSNWGLCNHVTKVLRRTPGLRTAILILIALRGLPA
                     GYAASESPPDKPAEALYLQLGQAGLDPARVYQVRDAALDRPSVHITLEDGTIAFTQDV
                     MGRITGAFFEGDGEVLLTPPTEVERRSMSLFTGMAILEEHFATAYFRFNDDVPAELRP
                     DLRSTENKQEFVDRWGTTVRNLSNIDAMRLLITFSRFLPAKSGADSATSALRAAESDR
                     FLHARLQGTRLGVFDVDFDARSAEQVEAGQEKTAENGVTYYDVWTSFSPPAARSAQSL
                     NAGESPDREAPLEDWIAVRRYLI"
BASE COUNT          567 a          763 c          892 g          518 t
ORIGIN      
        1 agcagccaat gaacgcgggg caggagcgga caccggcaga ggcttcgatc aggccacgat
       61 tgatgcggag acagagctcg gtcatggcgt cagattcgtt gatgtcgggc ttggagaaga
      121 gctcacagcc cagcttgcgg aagattccct gcgccgccga cagaatgctg cgtgtatcgt
      181 cacgccgccc ggccacgtcg agtaacccga ataaaacacg ctccgggctg acccgcaccg
      241 agtcgtaaaa atccccggct acgcgtttgc cggcgaagac ggccgcgatg tcagcgcctg
      301 cgatctttgg gaatacagtc gggacaggct cgaccgtggg cgcgacttcg ggagtgacgg
      361 agcgaaagag ggagcgcata gggctagcca gccttccgaa gctagcatac gaaccgatac
      421 cgggcaacgg cactaactgc cgtagcggtc ggcgaggtac ttggcgaggt cggattcgag
      481 ctggccgtag tcggagtgga tggtggctcg cagagcggcc tctgtcgaac ttccctggct
      541 gatgcgttgc aaaatgcgtt ggatatcgct catgccgtac gaatcgttga tataggacac
      601 ggcggcgagg gattcggcat aggccacgta agcttccacg ccggaaaagt tcatgaagct
      661 tccttccagc acgttcagcg ggatattgcg ttgcgccttg aacagcaggg cgagctggtg
      721 gccgtcacct ccaagattct ttggctcgac cagctgcgca ataccttcat tcagccaatg
      781 cgggcagcgt ccgccagaga gctgattaat aaaggagtgg gccagctcgt gtttgagaac
      841 ccgcgccagt tcgggcgtga tggtgctcag gccgcttacg ggaatccgca gcttgccgtc
      901 gttgatcgcc ccggaccacg taggagcgtg agtaacgtcg aagaatgcct gctcggtata
      961 gagcgttacg agaatactgt cgcggggtgg attgccgagg tcgcgggaga gatcgtcata
     1021 gtcggattca agcgccgcga cgagctggga gcggaacgat tcagaaacct gcttgccctc
     1081 atagtgcaga acgaaatgac tgctttcgtg ctggctgaag tcggactcga ccgcatcctc
     1141 gcgctgcgcc ttggccagga actgctgcac ggccgcgtca ggccgcaatt ccagggacct
     1201 cttccaggaa gcgactgcct ctttggtgcg gtcggacgcg aactgggcgt agccgagcat
     1261 agtgtaggca tcgggtgagc tgggcgacga gcgtacggca cgctgagcat agggcagcgc
     1321 ttcggccgcg ttgccggtgc ggaccagcaa cgccgagtaa taaatgagga tggtggaatt
     1381 ctcaggttgg tagcggagag cgctctcaaa gtaactgcgg gcctgggcga tgttgccgtg
     1441 ttcgaactcg aactttccgg cgatgaagct ggcggtggcg ctcaactcgg cgttgccctt
     1501 ggcttccaga gccgcaagca cgtcgggatc gactctgcct tccttgataa tcttcgagaa
     1561 aaggtcgccc tcattggcca aactgtcggc aggagtgaaa gccggaagat ccgcaacctt
     1621 ggccgtcccg gccgcggagt gaatgggcat gccgccggca tccacgtggt cgaccagaga
     1681 cttgggaatg gcgtaagagt cgtcgccgac atcgtattcg tagtgggcgc cgttctcgcg
     1741 gacgtggtcc gcgacgatcg aacggccgtt cttgagatgg atggtgtcag ctcgtgcgcc
     1801 gtacaggctc agcacaagcc atacgacgag gatcagaaga aaagtgacac gcacccgcat
     1861 tcaggcgcat tctagcaact gggggctgtg caaccacgtg acgaaggtac tacgcagaac
     1921 gccaggcctg cggaccgcga ttttgatcct gattgcactc cgcgggctgc cggcggggta
     1981 tgcggcgagt gaaagtcctc ccgacaagcc ggctgaagcg ctctacctcc agctcgggca
     2041 agccgggctg gatccggcgc gcgtgtatca ggtgagggat gcggcgctgg atcggccttc
     2101 cgtccacatt actctggagg acggcaccat tgcctttaca caggatgtga tgggccgcat
     2161 cacgggagcg ttcttcgagg gagacggcga agtgctgctc actcctccga ccgaggtgga
     2221 gaggagatcc atgagccttt tcaccggaat ggcgattctg gaagagcact ttgccacggc
     2281 gtacttccgg ttcaatgacg acgttccggc agagctgcgg cccgacctgc gatccaccga
     2341 gaacaaacag gagttcgtgg accgctgggg aacgaccgtg cggaatcttt ctaatatcga
     2401 cgcaatgaga ctgctgataa ccttcagccg gtttctgcct gccaaaagcg gcgccgattc
     2461 tgctaccagc gcgcttcgtg cagctgagag cgaccgattc ctccatgcgc gtttgcaagg
     2521 aaccaggttg ggtgtgtttg acgttgattt tgacgccaga agcgcggagc aagtcgaagc
     2581 ggggcaagag aagacggcgg aaaatggcgt gacctactac gacgtttgga cctctttttc
     2641 tccgcctgct gcccgaagtg cacaaagcct caacgctgga gaatccccgg atagggaggc
     2701 gccgctggaa gactggatcg cggtgcgccg ctacctcatc
//
LOCUS       DASPUL010000296         3795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1967386, whole genome shotgun sequence.
ACCESSION   DASPUL010000296 DASPUL010000000
VERSION     DASPUL010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967386"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..847
                     /locus_tag="VEJ00_07285"
     CDS             443..847
                     /locus_tag="VEJ00_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX00999.1"
                     /translation="MKGFHCALARTATGMAVGGIATHQLFHLREAAAFTLKATTMAER
                     LGSVSVAVILFVFLAYLAFYFFNRSFFHGFIVAGGLILSFDIVLLHWIFQLHRITSGP
                     EADVLEPLFVLCGIALVITGVKRELAAGQRDA"
     gene            complement(981..1391)
                     /locus_tag="VEJ00_07290"
     CDS             complement(981..1391)
                     /locus_tag="VEJ00_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01000.1"
                     /translation="MELNSYRALALTFGRLLFGAVLLMGTAAHVVWAQAPAEIEATLK
                     QMLVATENRRLDAFVAQGDASFQADITPAMFNNFSAQVGPRLRQGYTASFLTQLRQQS
                     YVVYVWKLEFKDGGDDILFSLAVKGGKVGGFSLQ"
     gene            1839..2939
                     /locus_tag="VEJ00_07295"
     CDS             1839..2939
                     /locus_tag="VEJ00_07295"
                     /inference="COORDINATES: protein motif:HMM:NF015457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01001.1"
                     /translation="MVSSPRALDVAMRGGGQYSTAAKGAKDAIDSATPLVGEAISRLP
                     DGDISTPFTLVDIGCAGGGTSLDMVDEAIAAVRGRWPQRPICIVYTDQPRNDYNSLFR
                     LIHGLTPVPSYRDHYEGVYVLASATSFYEQMLPPGTVDLGFSATAMHWLSRTPCDLTQ
                     HVHSLCASGAELTTFARQAQQDWETILLQRTRELKPGGRLVLVNFCKDETGRYTSRVQ
                     MFDTVDALWRGFVTDGLISGEEYVRMTLPQYYRTVEECTRHLTDSASPVYRAGMRVEQ
                     CETRAIPCPFAAAFRQHGDAGRLARDATLALRSWTESTFLGALSSDRAAEERQSIIER
                     YYDAHEAFLRDNPTLYRADYVEIYMTIAKTAA"
     gene            3094..3312
                     /locus_tag="VEJ00_07300"
     CDS             3094..3312
                     /locus_tag="VEJ00_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01002.1"
                     /translation="MSLSATSSHTARLRKWPPHFQLYQALDAAGIPVKLDLYGAMIHN
                     FQDRILDAPEAILARRKMRTFLHQQLGM"
     gene            3465..>3795
                     /locus_tag="VEJ00_07305"
     CDS             3465..>3795
                     /locus_tag="VEJ00_07305"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HXX01003.1"
                     /translation="MATKRRSKAASTTARTAETAAGFHARSERLEAGKTLRGRVARES
                     HAAWRAPRRGRDPVEILQISSRGRLPELVPIRYGRMARSPFTFLRGSAGLMAHDLATT
                     PTTGVRVQ"
BASE COUNT          721 a         1116 c         1149 g          809 t
ORIGIN      
        1 tactaatcga tctgcttgaa gccagggcag ttgagctcgc cggctagttc gcatattcct
       61 ctatttgcat cttcaccctc ctgcgtctca aagccccaag cgagggctag cgtcatcaag
      121 gcgaaagcgg ccggtggaca attcgataga catgtgcgct acggagtgga aagagaaacg
      181 gccccgggtc ggggccaatc tccctcgcag cggaaactcc agtttagaac cgtctacttg
      241 acggggaagc tcatgggctc gtcgggacgc ccgccgcatc acgcgctatc atgtaacagg
      301 ggtaggaagc ctgccgatgc gcttgcaggc cccgtttctg agaacgacag ctttggttct
      361 ggcggcaaat ttgcgcggtc tgcttatgag caacgcttta ttcgctgtcg cttataggat
      421 gtctactttt cgttaggcgc taatgaaagg ctttcattgc gctctcgcta gaacagccac
      481 cggtatggcg gttggaggga tagcgacgca tcaattattt cacttgcgcg aggcggctgc
      541 gtttaccctg aaggcaacga ccatggcgga gcgtttgggc tccgtttctg tcgccgtcat
      601 cctgtttgtc ttcctggcct acctagcgtt ttatttcttc aataggtcct tcttccacgg
      661 cttcatagta gcgggtggac ttattctgag ctttgatatt gtgttgctgc actggatatt
      721 tcaattgcat cgcatcacga gtggcccgga ggctgacgta ttggagccgt tgtttgtact
      781 gtgcggcata gctcttgtaa tcaccggggt caaacgtgaa ttggccgcgg gccagcgtga
      841 cgcctaagct ctcgctcagg cggaccgctc gccagcggcg ctaaacaacg tccgctttcg
      901 ggagggccgg atgtccgcga tcggcccgac gcggtcgtac catgatgatg tcgccgacag
      961 cagtcgtccg cccgcgctgg ctattgcagg gaaaagcctc caaccttgcc gcccttgacg
     1021 gctagactga agaggatgtc gtcgccaccg tccttgaact cgagcttcca aacgtacaca
     1081 acgtaacttt gctgacgtaa ctgggtcaga aaactcgccg tatagccctg tctgaggcgt
     1141 ggtccgacct gcgcgctgaa attgttgaac attgccggag tgatgtcggc ttggaatgac
     1201 gcgtcgcctt gagcgacgaa agcatctaac ctcctgtttt cggtcgctac aagcatttgc
     1261 ttcaatgttg cttcgatttc tgcgggtgct tgcgcccata caacgtgcgc agcagtgccc
     1321 attaggagca cggcgccgaa aaggagccgt ccgaacgtca gcgccagagc gcggtacgag
     1381 tttaactcca tggcttgctc gcggttggct ggatgatacg ccgaccggga ccgcaatagg
     1441 cttcaccggc ggggaagcgg cgaatccgta taagcgcggg caggcagcta tcgaccagaa
     1501 gcggccctcg gcgcggacgt tgcgattggg cgaactctat tcttgatctc catcgctaga
     1561 ggttgggcca gtgttgctgg tccatccacg caggtgccga tggacgccag tggaacgtcg
     1621 gggtagcctt accgccgaac ctattttgcc tccgaccggc gcgacgtgcc tttaggttgg
     1681 tatggggagt aacgtcgttg agtctcgggc gcttttcgcc gctcggatat tcgttaaaat
     1741 cgatgaccgc cgcttcattt tccgtcagtt gtagggcggc tactcttcgt tagagtttgc
     1801 catagtcaac aggactctag ctgacggaga aacgcctcat ggtgagtagc ccgcgggctc
     1861 tagacgtcgc aatgcgcggc ggcgggcagt actccaccgc tgccaagggc gccaaggacg
     1921 cgatcgacag cgcaacgccg ctggtgggtg aagcgatctc tcgtctacct gacggggata
     1981 tttcgactcc gttcacgcta gtggatattg gttgcgccgg cgggggaacc tcgctcgaca
     2041 tggtggacga agccatcgcg gccgtgcggg gtcgctggcc ccaacgaccg atctgcattg
     2101 tttatacgga ccaaccgcga aacgattaca acagcctatt ccgcctgatc catggcctga
     2161 ccccggtgcc aagctatcgt gatcattacg aaggggtgta cgtactggcc tcggcaacct
     2221 cgttctatga acagatgctc ccgccgggga cggtagacct gggcttctcg gcgactgcga
     2281 tgcattggct tagccgtacg ccctgcgatc ttacccagca tgtccatagc ctttgtgcgt
     2341 cgggtgccga acttaccacc tttgctcgac aggcacagca ggactgggaa accatcctcc
     2401 tccagcgaac gcgagagctc aagccaggcg ggcgcttggt acttgtcaat ttctgcaagg
     2461 acgaaaccgg gcgctacacg agccgggtcc agatgttcga caccgtcgat gcgctgtggc
     2521 ggggttttgt caccgacgga ctcatcagcg gtgaggaata tgtgcgaatg accctgcctc
     2581 agtactacag gaccgttgaa gagtgcacac ggcacctgac cgactctgcc tcccctgttt
     2641 atcgagcagg catgcgagtt gagcagtgcg agacacgcgc catcccgtgt ccgtttgctg
     2701 ccgccttccg ccagcacggc gacgccggac gccttgcccg ggacgccacc ctggctctgc
     2761 ggtcgtggac cgagagcacg ttcctgggtg ccctatcctc ggatcgagcg gctgaggagc
     2821 gccagtcaat cattgagcgt tactacgatg ctcatgaggc gttcttgcgc gacaacccca
     2881 cgctgtaccg agctgactac gtggagatat acatgacgat tgcgaaaacc gcggcgtagc
     2941 gtgtggttat gagtttgcgg aggaaacctg attatcgatt gcagaggacg gccctcgcgc
     3001 tgcttgccgc gccagccgtc agtttgatct gctgactgtc cgcttccggg acgtccgacc
     3061 gtctggtcag ggtcgtaaac gggacatgct gagttgtcgc tatcggccac ttccagtcat
     3121 accgcccgtc tgcgaaaatg gccgccgcat tttcagcttt accaagctct agacgcggcc
     3181 ggaatcccgg taaaactcga tctctacggg gcgatgatcc acaattttca ggaccggatt
     3241 ctggacgcgc ccgaggcgat cctcgcgcgc cgtaagatgc gaactttcct gcatcaacag
     3301 cttggcatgt gaacttggcc agtcaagggc gctgctgacc gcgatccttg aatgacggct
     3361 ctctggtatt ggcggtgtca gctcagggtc ggctggaagg gtaagctgac gtcggcatac
     3421 ctgccagcgt cgcggcggcg actccgacgc accaaggaaa gatgatggcg acgaagaggc
     3481 gatcgaaagc agccagcacc actgcacgta ccgcggaaac cgccgccggc tttcacgccc
     3541 gttcggaacg gctcgaggct ggaaagaccc tgcgcgggcg tgtggcgcgc gagagccacg
     3601 cagcctggag agccccacgc cggggcaggg accccgtcga gatcctgcag atctcgagtc
     3661 gcgggcggct cccggagctg gtccccatcc gctacgggcg catggcgcgc agccccttta
     3721 ccttcctgcg cggctctgcg gggctgatgg cgcatgatct cgccacgacg ccgaccacgg
     3781 gcgtgcgggt gcagg
//
LOCUS       DASPUL010000297         4679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_735622, whole genome shotgun sequence.
ACCESSION   DASPUL010000297 DASPUL010000000
VERSION     DASPUL010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735622"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..1175
                     /gene="mdh"
                     /locus_tag="VEJ00_07310"
     CDS             249..1175
                     /gene="mdh"
                     /locus_tag="VEJ00_07310"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HXX01004.1"
                     /translation="MRNKVTIVGSGNVGATAAHWIASKELADVVLIDIIEGVPQGKGL
                     DLLEAMPIEKRDSHVMGTNDYRDTVNSDIVVITAGIPRKPGMSRDDLLNTNHGIMKAV
                     VGEVVKHSPNCILIVVSNPLDAMAQAAFKLCGFPRERVIGMAGVLDSARFRAFIADEL
                     QVSVENVTAFVLGGHGDTMVPLPRYSTVAGIPITELIEPAKLEALVKRTRDGGAEIVK
                     YLKTGSAYYAPSAATTEMVEAILKDKKKILPCAAYLQGEYGINGYFVGVPVKLGTRGI
                     EQIIEIKLTPEEKTALDKSAAAVKELCGVIGV"
     gene            1191..2042
                     /locus_tag="VEJ00_07315"
     CDS             1191..2042
                     /locus_tag="VEJ00_07315"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HXX01005.1"
                     /translation="MIPSHQELEVPLLKALADKGGKAKPGEMYEALSRMLGLTDADLA
                     ETLPTGDNRFRNRVRWARQILIDKGDMYRADYGVWGLTEAGRARLQGRVQPGRKGARD
                     GRAVVPKLDAAIALPNLEEMADNYASVFERKILEELRDHEPHEFEQFAAKLLSAYGFR
                     NVKVTHTRTAPDGGIDGNGELKAGLVNMRAAFQCKRWQGVVGRPEIDKFRGAIQGKFE
                     HGYFFTTSTFSEEAREASIRDGAVPIFLFDGHEIIQIMIEKGLGIIRRPIEIYEDRVD
                     TLFEKDE"
     gene            complement(2120..2647)
                     /locus_tag="VEJ00_07320"
     CDS             complement(2120..2647)
                     /locus_tag="VEJ00_07320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXX01006.1"
                     /translation="MTRQPGLYATFETSEGTIVCRLFEKDAPKTVANFVELAEGKREW
                     THPSTRKKSKDRLYDGTIFHRVIPDFMVQGGDPQGTGMGGPGYQFEDETKGSPHKFDK
                     PGKLAMANAGPNTNGSQFFITVVPTTWLTGKHTIFGEVVEGQDVVDKITKLPRGRNDR
                     PSKDVVLKKVGIERV"
     gene            complement(2771..3520)
                     /locus_tag="VEJ00_07325"
     CDS             complement(2771..3520)
                     /locus_tag="VEJ00_07325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXX01007.1"
                     /translation="MRTALFLTLLTAMLAFGQSNSAAQAAPATKPSTSTKTTTAAKPA
                     VSAAAKPTAIIDTTAGKMTCTLFPDKAPIGVENFIGLATGTRDWKNPVSGAMKHGVPL
                     YDGTVFHRVIPGFMIQGGDPAGNGSGDPGYKFKNEVSSDLLFDRPGRLAYANAGPNTN
                     GSQFFITEVATPHLNGNYTIFGQCDDATVALVKQIAHMATDPQNDRPFRPVKINHITI
                     TKGGAAAKPAGAAVKKPTAATAPKTANPADK"
     gene            complement(3622..>4679)
                     /locus_tag="VEJ00_07330"
     CDS             complement(3622..>4679)
                     /locus_tag="VEJ00_07330"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HXX01008.1"
                     /translation="NDPGHISLDKIRRGNLGAEFFSIWVEPETNEGHFARHTFDLIDS
                     VYEQTTRHPDRMTMAFSVADIERAHAEHKLAALMGIEGGHSIENDIHLLRDYYRLGVR
                     YMTLSWSNTNEWADASGDINDPKVQHHNGLTDFGKQIVLEMNRLGMIVDISHVADKTF
                     WDTIAVSKAPVIASHSSARALCNAPRNMTDDMLRAVAKNGGVVDVNFFSGFLDQAYWD
                     ASRAQSKERDAAVQGMLAKMRAEGKSVTYLDEDRLQREWTAKIPRPSFSVLIDHIDHI
                     AKVAGVDHVGLGSDFDGVSGATPEGMDSAADLPKITQALLDRGYSEGDIRKILGGNVL
                     RVFSDVERVSREMKSAK"
BASE COUNT          950 a         1331 c         1408 g          990 t
ORIGIN      
        1 agcatggagc gcacaatcga gcagaagaaa gttacatacg acttcgagcg cctgatgccg
       61 ggcgccacca aggtcaagac cagcgagttt gccggttaca tgatcgagaa catgggcgcc
      121 ggagtgttgg tgtaagttcc attttcggga aaagtccttg aaagggcgcg gcttccagct
      181 gcgccgccaa aagcgagaga tattctcagg gctttagccc ttgaggtcct ttatagaaag
      241 gaattcatat gcgcaacaaa gtaaccatcg taggctcagg caacgtgggt gcaacggccg
      301 cccactggat cgcctccaag gaactcgccg acgtcgttct catcgacatc atcgagggcg
      361 tcccgcaggg caaaggactc gacttgcttg aggccatgcc catcgagaag cgcgactcgc
      421 atgtcatggg caccaatgac tacagggaca cggtcaactc cgacattgtc gtgatcacag
      481 ccggcatccc gcgcaagccc ggcatgagcc gtgacgacct cctcaacacg aatcacggca
      541 tcatgaaggc agtggtgggc gaggtggtta agcattcgcc gaactgcatt ctgattgtgg
      601 tttccaaccc gctggatgct atggcccaag ccgctttcaa actctgtgga ttcccgcgcg
      661 aacgcgtcat cggcatggct ggcgtgctcg attcggcacg cttccgcgcc ttcatcgccg
      721 acgaattgca ggtcagcgtg gagaacgtca ctgcattcgt gctcggcggt cacggcgaca
      781 ccatggtgcc gctgccgcgc tattcgacgg tcgctggcat cccaatcacg gagctgatcg
      841 aaccggcgaa gctggaagca ctggtgaagc gcacgcgcga cggcggcgcc gaaatcgtaa
      901 agtacctcaa gaccggatcg gcttactacg ctccttcggc cgcgacaacg gaaatggtcg
      961 aagcgatcct gaaagacaag aagaagattc tgccctgcgc cgcgtatctg cagggtgaat
     1021 acgggatcaa cggctacttt gtcggcgtgc cggtgaaatt gggaacgaga ggcatcgagc
     1081 agatcatcga gatcaagctg acgccggaag agaaaaccgc cctcgacaag agcgccgcgg
     1141 cggtgaagga gttgtgcggc gtgatcggag tgtgaggagg tgtccgattc atgataccca
     1201 gccatcagga actcgaagtc ccactgctta aggcgctggc tgacaaggga ggaaaagcaa
     1261 aacccggcga gatgtacgaa gcgttgagca gaatgctggg tttgaccgac gctgacctcg
     1321 cggagacttt gccgactggc gataatcggt ttcgaaatcg agttcgatgg gcccgccaaa
     1381 tccttatcga caagggcgac atgtatcgcg ccgactacgg ggtttggggc ctgaccgagg
     1441 ccgggagagc ccggctccag ggcagagttc agccggggag gaagggagcc cgcgacggtc
     1501 gtgccgttgt acctaagcta gacgcagcta tagccttgcc aaacctcgag gaaatggcgg
     1561 acaactacgc gtcggtgttt gagaggaaaa tcttggagga gttgagagac cacgagcccc
     1621 acgaatttga gcaattcgct gcaaagctac tgagcgcata tggcttccgg aatgtgaagg
     1681 tgacacacac acgaacggct ccagacgggg gaatcgacgg taacggggaa ttgaaggccg
     1741 gcttggtgaa tatgcgagca gcgttccaat gcaagcgatg gcagggtgtc gtcgggcgtc
     1801 ccgagataga taagtttcgg ggggcgatcc aagggaagtt tgaacacgga tatttcttca
     1861 ctacctcgac attttcggag gaggctcggg aagcatcgat tcgggatggt gcagtgccta
     1921 tatttctttt tgatgggcac gagatcatac aaatcatgat tgagaaaggc ctggggatca
     1981 tacgcaggcc catcgagatt tatgaggatc gcgtggatac tctcttcgaa aaggacgaat
     2041 agcactcctc ccttgagccg caagcatgct gaacgtctgg cagagctaac tgttcgggca
     2101 ggacaggcag gctcgctcgt cacacccttt caatccccac cttcttcagc accacatcct
     2161 tgctcggccg atcgttgcgg ccgcgcggga gtttcgtaat cttatccacc acgtcttgcc
     2221 cctcaacgac ctctccaaag atggtgtgct tgccggtcag ccacgtcgtc ggcacgacgg
     2281 tgataaaaaa ctgcgagccg ttcgtgttcg ggcccgcgtt ggccatggcc agcttgccgg
     2341 gcttgtcgaa tttgtgcggc gaacctttgg tctcgtcttc gaactgatat ccggggccac
     2401 ccatgcccgt gccctgcggg tctccgccct ggaccatgaa gtcggggatc acgcggtgaa
     2461 agatcgtccc gtcgtagagg cggtctttcg atttcttgcg ggtcgagggg tgcgtccact
     2521 cccgctttcc ttccgcaagc tcgacgaagt tggcgacggt ttttggtgcg tctttctcga
     2581 acagccggca aacaatcgtt ccttcgctgg tctcaaacgt ggcataaagt ccgggttggc
     2641 gagtcatgag gtcctttcag agagtgtcgt tagtcttcgg tcgttagtcg ttagcaagag
     2701 cgatcaatgt agggatagcg ggtgccccat cctaaccgcg ttctgtgcgg ttagggtggg
     2761 gactttgacg ttacttgtcc gcgggatttg ccgtcttggg tgcagtcgct gccgtcggct
     2821 tcttcacagc cgcccccgcg ggcttcgccg ccgctccacc cttggtgatc gtaatgtggt
     2881 tgatcttcac cggccgaaat ggccggtcgt tctgcggatc ggtcgccatg tgcgcgatct
     2941 gcttcaccag agccaccgtc gcatcgtcac actgcccgaa gatcgtgtag ttgccgttga
     3001 gatgcggcgt cgcgacctcc gttatgaaga actgcgagcc gttcgtgttt ggtccggcgt
     3061 tggcataggc caggcgcccc ggccggtcga aaagcaggtc ggatgacact tcgttcttga
     3121 atttgtagcc tgggtcgccg gatccgttcc cggcagggtc tccaccctga atcatgaagc
     3181 cggggatcac gcggtgaaag accgtcccgt cgtacagcgg cacgccgtgt ttcatggctc
     3241 cgctgaccgg attcttccag tcccgcgtcc cggtggccag gccgatgaaa ttctcgaccc
     3301 cgatcggcgc cttgtcgggg aacagcgtgc aggtcatttt gcccgccgtc gtgtcgatga
     3361 tcgccgtagg cttggcagcg gcggaaacgg ctggtttcgc tgccgtcgtc gttttagtgc
     3421 tagtgctcgg tttagtcgcg ggagctgctt gcgctgcgga gttcgactgt ccgaacgcca
     3481 gcatcgccgt tagcaacgtc aggaagagtg cggtgcgcat gaacgctcct cggagccgga
     3541 gatgttcctt caactttctg ggcaacgttg cattgtacga cagccaagac ctcggcttct
     3601 cctggagagc gagcggcgag actacttggc tgacttcatc tcgcggctta cgcgttccac
     3661 gtcactaaac acgcgcagca cattcccgcc aaggatcttc ctgatgtcgc cttcactata
     3721 accacgatcc agcaaggcct gcgtgatctt cggcaggtcg gcggctgaat ccataccttc
     3781 aggcgttgcg ccgctgacgc cgtcgaaatc agagcccaac cccacatgat cgactcctgc
     3841 cactttggcg atgtggtcga tgtggtcgat gagcacgctg aacgacgggc ggggaatctt
     3901 cgccgtccac tcccgttgca gccggtcttc gtccaaataa gtgacagact tcccctcggc
     3961 tctcatcttc gcaagcatac cctgcaccgc cgcatcgcgc tctttggact gcgcgcggga
     4021 agcatcccag taggcctggt ccagaaagcc ggaaaaaaaa ttcacatcga ccacgccccc
     4081 gtttttagcc acggcgcgca gcatgtcgtc ggtcatgttg cgcggcgcgt tacacagcgc
     4141 gcgggcggac gaatgcgatg cgatcaccgg agccttgctc actgcgatgg tatcccagaa
     4201 cgtcttgtcg gcgacgtgcg agatatccac aatcatgccc agccgattca tttcgagcac
     4261 gatctgcttg ccgaagtcgg tcagaccgtt gtgatgctgc accttcggat cgttgatgtc
     4321 tcccgaagca tctgcccact cattcgtgtt cgaccaggac agcgtcatat agcggacacc
     4381 caggcggtag tagtcccgca gcaggtgaat gtcgttctcg atggagtgac cgccttcgat
     4441 gcccatcagc gccgcgagtt tatgctcggc gtgagcgcgc tcgatatcgg cgacggagaa
     4501 tgccatcgtc atgcgatcgg gatgccgtgt tgtttgctcg tatacggagt caatcagatc
     4561 gaacgtgtgc cgggcgaagt ggccttcatt agtttccggt tccacccaaa tcgagaaaaa
     4621 ttccgcgccc aggtttccgc ggcggatctt gtcgaggctg atgtggccgg gatcgttcg
//
LOCUS       DASPUL010000298         6569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_194067, whole genome shotgun sequence.
ACCESSION   DASPUL010000298 DASPUL010000000
VERSION     DASPUL010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194067"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VEJ00_07335"
     CDS             <1..186
                     /locus_tag="VEJ00_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HXX01009.1"
                     /translation="GPLSTMVADLLSEESLRARGLYDPAYVSGLIARDKQGLEDNAYQ
                     IWTLLTNEIWFRTFFQN"
     gene            231..2393
                     /locus_tag="VEJ00_07340"
     CDS             231..2393
                     /locus_tag="VEJ00_07340"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HXX01010.1"
                     /translation="MLQIIQYQKTGEIYVDELPAPKLRPGNVIVRNAFSLISAGTERT
                     SVETAQASMVQKARMRPDLVRQVIDNFHREGLVATYNKVRDRLDNFKDLGYASAGVVI
                     ESGVDDIKVGDRVACAGVGYASHAEIVSIPRNLVVKVPDEVGLDEAAFTTVCAIAMQG
                     VRQADVRVGEQVVVIGLGLIGLITTQLLKASGCRVLAMDVAPRNFDLALQLGCDRCVL
                     SNDDAVAEVQTLTRGYGADAVVITAATTSNQPVELAIQCARKRGTVVAVGAVGMNIPR
                     SPFYEKEISFRMSCSYGPGRYDPEYEERGHDYPLGFVRWTENRNMEAVLDMMALGKLN
                     VGALITHRIPVDQGLRAYEIITGKIEESYLGILIQYPDPSTPIAASRRVELGGATRRV
                     APGQRAVLGLIGAGNFTQSMLLPPLMKLAPRMRGLATGKPVNAKNIGKKYGFEFCATD
                     ASEIIEDKETNLVFVTSRHDSHARYVAAALRGGKSVFVEKPPALNNEELDSILDAYGE
                     AERSGAAPQLMVGYNRRFSEPMLAIQKLFAGRTEPLVMHYRVNAGFMPSTHWMQHPDQ
                     GGRFIGEGGHFVDVMQFLCGALPVSVYAVEPTDAARRYNHDNILVSIVFADGSAGTIH
                     YLANGASTVEKEYLEVFGAGKTARLWNFKKLECAAGRKKSTSSFSGDKGHSAEMKALL
                     QGFESGKGSPIGLDSLVATSRVTFAVLDSLRTGLVVNL"
     gene            2573..5359
                     /locus_tag="VEJ00_07345"
     CDS             2573..5359
                     /locus_tag="VEJ00_07345"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01011.1"
                     /translation="MKNKRVSLPAVCGALALLLTVGTSLGWAQGQSKAFVVRATKHAV
                     APPLSQMPLIPPQAAQLNPFEAARTRSEESALVHGLNSSRSFQSAALQGSADATAAAQ
                     LSTNAGVDILGLGAGFSGFSASNNIPSANGAAGTTQFVQFANDAFAVFNKSNGSLAFG
                     PASINTLWQALGAPCSSNPNMDSIVQFDQLANVWVILMPLYTTPPHFCIAVSTTSDAT
                     GSWNLYVFEAPVNTQLCHCRMMPDYPKLALWPDAYYISYHQAWNLNYEGPVACAVNRN
                     AMLSGSAATMQCFANTGTGNDAWLPSDLDGATPPPTGSPNYFLGFDPNDQSLDLWQFH
                     VDWTNPANSTLTGPTNIPVAPFLEPCGDTVTVFTPQDNCVPQAGTSNMLGAFGDEVMY
                     RLAYRNFGSSQSLVVNHTVQVASGSNQTGLRWYELQNTGSGFGLFQQGTYAPDSNYRW
                     MGSIAMDKVGDIALGYNVSSSSMSPSIRYTGRLSTDTLGTMEGEVDVLSSAGISPGSQ
                     TNTPRWGDYSSLAVDPTDDCTFWYTTEYMPANGGNWGTRIASFVFPSCIPNFTLSVAE
                     VGHGTVTSSDGAINCTNGTGSCSAVYPSGTAVTLNAAGATGWALSGWSGACTGGNPCN
                     LVMNSNLSATATFATTTQSWTLSVSESGQGTVTSTDGTINCANGSGTCSALYLSGSSV
                     TLNASAPSGWAFSGWSGPCSGSGSCNLQVTGNTTATATFTTSWAIVNKTSRQGNPLTS
                     LTIPATGSGNLIAIALMFNGTTSVASVTDNAGNAYVSAGARSTVNQYTAEIWYAVNAK
                     PGATVVTPTYNGSPTHMEITEWEVSGLHNGPPDKTAVISQVKVLTNNAAGAPATTTQL
                     GDFVVSILLANTSKFTTISSGNEFTDDFNINGNGWAHLTNDLAAPGTHQASWFTSSPA
                     GSYCSSTVAFLPGP"
     gene            5598..>6569
                     /locus_tag="VEJ00_07350"
     CDS             5598..>6569
                     /locus_tag="VEJ00_07350"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXX01012.1"
                     /translation="MFDHSPWSVLCRSVFVTVLIISCSAVLSYAGTVSIHPGQDIPTV
                     VSQNPAGTTYLIYPGTYRMTQHIVPKTGDSFIGQTACAPPATACPAILSGSVVIGSLA
                     KFNGSIYEVTGQTQQGSMPFSNGICQPGYGACNRPEDLFFDGVPYQHLSATVLPTIGP
                     KQWWFDYTNNIIYFSDNPAGHTVETSVLDTAFASTANNVTIQYLTVEGFAAPLQRAGV
                     ESSSTNLQPNVSANWVIKNCEILNNHGGGVRMGYGMQVLNSYVHDNGIVGIAGGTNSP
                     TASGIVIQGNTINHNNYAKGLSDWGAGGFKVGQTANLVFRGNTVSNND"
BASE COUNT         1330 a         1978 c         1912 g         1349 t
ORIGIN      
        1 ggaccgctgt cgaccatggt tgccgatcta ctctccgagg aatccctgcg ggcccgcggc
       61 ctctacgatc cggcctacgt ttccggattg attgcccgcg acaagcaggg attggaggat
      121 aatgcttacc agatctggac attattgacg aacgagatct ggttccgtac gttcttccag
      181 aactgacgat gatcgagttc cgagaccacg taatcgaaag gacgaactag ttgcttcaga
      241 tcattcaata ccagaagacg ggcgagattt atgtcgatga gctgcccgcg ccgaaactgc
      301 gcccggggaa tgtgattgtt cgcaatgctt tttcgctgat cagcgccggc accgaacgca
      361 cgtcggtgga gacggcccag gcgtcgatgg tgcagaaagc ccgtatgcgg cccgatctgg
      421 tccgccaggt tatcgacaac ttccaccgcg aaggcctggt cgcgacttat aacaaagtcc
      481 gcgaccgtct ggataatttc aaggacctcg gctacgcctc agcaggggtg gtcattgaat
      541 ccggcgtgga cgacatcaag gtcggcgacc gtgtcgcgtg cgccggcgtt ggctatgcct
      601 cccacgccga gatcgtctcg attccgcgca accttgtcgt caaagtgcct gacgaggtcg
      661 gcctcgatga ggcagccttc accaccgtct gcgcgattgc catgcaaggt gtgcgccagg
      721 ctgatgtgcg cgtcggcgaa caggtcgttg tgatcggact ggggttgatc ggcctgatca
      781 ccactcagtt gctgaaggcc agcggatgcc gcgtgctggc catggatgtt gctccgcgga
      841 atttcgatct tgcgctgcag ctcggatgtg accgctgcgt cctgagcaat gacgatgccg
      901 tcgccgaggt acagaccctc acgcgcgggt acggagccga tgccgtagta attaccgcag
      961 ccaccacctc gaaccagcct gtggaactgg ccattcagtg cgcccgcaag cggggcactg
     1021 tcgtggccgt cggcgccgtg ggaatgaaca tcccgcgctc gcccttctac gagaaggaga
     1081 tcagcttccg catgtcgtgc tcctacggcc caggccgata cgatcccgag tacgaggagc
     1141 gcggacacga ctatccgctg ggatttgtcc gctggaccga gaaccgcaac atggaagccg
     1201 tgcttgacat gatggcgctc gggaaactca acgtgggggc gttgattacg caccgcattc
     1261 ccgttgatca gggcttgcgc gcctacgaga tcatcaccgg caaaatcgag gagagctatc
     1321 tcggcattct gatccagtat cccgatccga gcacgccgat cgcagcctcc cgccgcgtcg
     1381 agctgggtgg cgcgactcgc cgcgtggctc ccggacagcg tgcagtcctg ggcctcattg
     1441 gcgcgggcaa cttcacccag tcgatgctcc tgccgccctt gatgaaactg gctccccgga
     1501 tgcgcggtct ggctaccggc aagcccgtga acgccaagaa tattggcaag aagtacggct
     1561 ttgaattctg cgccaccgat gccagcgaga tcatcgagga caaggaaacg aatctcgtat
     1621 tcgtcacctc ccgccacgac tcgcatgcgc ggtatgtggc tgcggcgttg cgcgggggca
     1681 agagtgtgtt tgtcgaaaaa ccgccggcgc tcaacaacga ggaactcgac tctatcctgg
     1741 acgcgtatgg cgaagccgaa cgttccggcg ctgcgcccca gcttatggtc ggctacaacc
     1801 ggcgcttctc cgagccgatg ttggcaatcc agaaactgtt tgcgggacgc acggagccgc
     1861 tggtgatgca ctatcgcgtc aacgccggtt tcatgccatc cacacactgg atgcagcatc
     1921 cggaccaggg tggccgcttc atcggcgagg gcggacactt cgtcgacgtc atgcagttcc
     1981 tctgtggagc gctgcccgtc agtgtttacg cggtggagcc caccgacgcc gcacgccgct
     2041 ataaccacga caatattctg gtttcgatcg tcttcgccga cggctctgcg ggaacaattc
     2101 actatctagc caacggggct agcaccgtcg aaaaggaata tctggaggtc tttggcgccg
     2161 gcaaaacagc ccgcctgtgg aacttcaaga agctggaatg cgccgccggg cggaagaaat
     2221 ccacgtcatc gttctcagga gacaaagggc actcggcgga aatgaaggcc ttgctgcagg
     2281 ggttcgagtc gggcaagggc tcacccatcg ggctggactc cctcgtggca acgtcccgcg
     2341 tgaccttcgc cgttttggat tccctgagga cgggccttgt agtaaacttg tgagcgcctc
     2401 cccaaggcgc tgtcgggccg ggtcatccgg cggtatcctc gcttgtccgt aagctcaaaa
     2461 gtaggttttt ggggactttt ccctttttct cgctcgcgtg caatcttagg ccgtggtgtc
     2521 gtcttgctgt cttccagtac caaagtaaaa cttgagactt gggattcact tcatgaaaaa
     2581 caaaagagtt tcgttgcctg ctgtttgtgg cgctctggcg ctgttgctga cggtggggac
     2641 atcccttggc tgggcccaag gtcaatcgaa ggctttcgtg gtaagagcca caaagcacgc
     2701 cgtcgcacct cctctgagcc agatgccgct gattcctccg caggcggcgc aactgaatcc
     2761 ctttgaagca gcgagaacga gaagcgagga gagcgcgttg gtgcatgggc tgaattcctc
     2821 ccgctcgttt cagagcgcgg ccctccaggg aagtgccgat gccaccgcag ctgcccaact
     2881 ttccacgaat gccggtgtcg atattcttgg tctcggagcc ggattctcag gtttctcggc
     2941 ctcaaacaac attccctcgg ccaacggagc cgccgggaca actcagtttg tgcagtttgc
     3001 taacgatgcc tttgcggtgt tcaacaaatc caacggcagt ctggcctttg gcccggccag
     3061 catcaacacc ttgtggcaag cgctgggcgc gccctgctct tcaaacccaa acatggactc
     3121 aatcgttcag ttcgaccagt tagccaatgt ttgggtcatt ctgatgcccc tttacacgac
     3181 tccgccacac ttttgtatcg ccgtttccac tacttcggac gcaaccggat catggaatct
     3241 ctacgttttt gaagcgccag tgaacacgca gctgtgccat tgcaggatga tgccggacta
     3301 tcccaagctg gcactgtggc cggatgccta ctacatttcc taccaccagg cctggaacct
     3361 gaattacgaa ggtccggtcg cctgtgccgt aaaccgcaat gccatgctca gtgggtctgc
     3421 cgctaccatg cagtgtttcg cgaacacggg cacgggcaac gatgcctggc tgccttcaga
     3481 cctggacggc gccacgccgc cgcccacggg ttcccccaac tacttccttg gtttcgatcc
     3541 caacgaccag tccctcgacc tctggcagtt ccacgtggat tggacgaacc cggcaaattc
     3601 cacgctcacc gggccgacga atattccggt ggcgcctttc ctcgagccct gtggcgacac
     3661 ggtcacggta ttcacgcccc aggacaattg tgtcccgcag gcgggaacct cgaatatgct
     3721 gggggccttc ggagacgaag tgatgtaccg cctcgcctac cggaactttg gaagttccca
     3781 atctcttgtc gtcaatcaca cagtgcaggt ggcgtcgggc agcaaccaga cgggcctccg
     3841 ctggtatgaa ctgcagaaca caggaagcgg gttcgggctc ttccagcaag ggacatacgc
     3901 cccggattcc aactaccgct ggatgggcag catcgccatg gacaaggtcg gtgacattgc
     3961 tctgggttat aacgtgtcca gcagcagcat gagcccgtcg attcgctaca ccggacgttt
     4021 atcgacagat actctgggca ccatggaggg tgaggtcgac gtgctgtcct cggccggcat
     4081 ctctccgggt tcgcaaacca ataccccccg ttggggcgac tacagcagcc tggccgtgga
     4141 tccgaccgac gactgcacgt tctggtatac gacggaatac atgccggcga acggtggcaa
     4201 ttggggcaca cggatcgctt ccttcgtctt cccgtcttgt atcccgaact tcacgctcag
     4261 cgtggcggaa gtggggcacg gaaccgtaac cagcagcgac ggtgcgatca actgcaccaa
     4321 tggcacgggc agctgcagcg cagtctatcc gagcggtacg gctgtgaccc tgaacgccgc
     4381 cggcgcgacc ggctgggcgc tctcgggatg gagtggagcc tgtaccgggg gcaatccctg
     4441 caatctggtg atgaacagca acctgagtgc aaccgccaca ttcgcgacaa ctacgcaaag
     4501 ctggacgctc tcggtgagcg aatcagggca gggcactgta accagtaccg acggtaccat
     4561 caactgcgcc aatgggagcg gcacgtgcag cgcgctttat ttgagtggca gttcggtgac
     4621 gctgaacgcc agcgcgccca gcggctgggc gttctcagga tggagtgggc cctgcagcgg
     4681 cagcggttcc tgcaatctgc aggtgacagg gaatacgacc gcgactgcta cgttcacgac
     4741 ctcctgggct atcgtgaaca agacctcgag gcagggaaat cccctcacca gcctgacgat
     4801 tccagccacc ggctccggga atctgatcgc cattgccctc atgttcaacg gcaccacttc
     4861 cgtggcgagt gtgaccgaca atgcgggtaa cgcttacgtg agtgcgggcg ctcgatccac
     4921 ggtcaatcag tatacggcgg agatttggta cgccgtcaat gcgaagcccg gtgccacggt
     4981 ggtgactccg acctataacg gttcaccgac tcacatggag attacggagt gggaggtgtc
     5041 gggtcttcac aatggcccgc cggacaagac tgccgtcatc tctcaggtaa aagtgcttac
     5101 caacaatgca gccggagcgc cggccacgac cacgcagctg ggtgattttg tcgtctcgat
     5161 cctgttggcg aatacgtcga agtttacgac catcagttcc ggcaacgagt ttacggatga
     5221 tttcaacatc aacggaaatg gctgggcgca tctcaccaac gatctggctg ctcccggcac
     5281 ccaccaggcg agttggttta cctccagtcc ggctggttcc tattgctcga gcacagtagc
     5341 ctttctcccc gggccctgat taccctaatt caaacagact agccccgtgg cccctgggcc
     5401 aatgctccag gggccttttt tcgcggtctt gcgcgaatgc gcgcgcagtg tcttgaaaac
     5461 tgagcatgta cctctgagac ctacctggat tttgacggga gccgatataa ccgacggtgg
     5521 cggctccggc gtgctgcggg tagtacaagt gccgacagca tccagccccg tcagatattc
     5581 ccccgagagg tgcgaacatg ttcgaccact ccccctggtc ggtgctgtgc aggagcgtct
     5641 ttgtaaccgt cctcatcatt tcttgtagtg cagtgctgag ttatgcggga actgtaagca
     5701 ttcatcctgg tcaggatatc ccgacggttg tttcgcagaa tcccgccgga actacttatc
     5761 ttatctatcc cggcacgtat cgaatgacgc aacacattgt gcccaagact ggcgacagtt
     5821 tcatcggaca gactgcgtgt gcaccgccgg cgactgcatg tcccgcgatc ctctccggct
     5881 cggtggtgat cgggtcatta gcgaaattca acggcagcat atacgaggtg acgggccaga
     5941 cgcagcaggg aagcatgcca ttttccaatg gcatctgcca gcccggctac ggtgcgtgca
     6001 accggccgga ggatctgttc tttgacggtg ttccctatca gcacctgtct gccacggtct
     6061 tgccgacgat cgggccgaag caatggtggt tcgactacac gaacaacatc atctacttct
     6121 ccgataatcc ggcgggtcac accgtcgaga ccagcgtgct ggatacggct tttgcctcca
     6181 cggctaacaa cgtcacgatt caatatctca ccgtggaggg ctttgcagct ccgctgcaac
     6241 gagcgggtgt cgagtccagc tccacgaatt tgcagccgaa tgtgagcgcc aattgggtga
     6301 tcaagaactg cgagatcctc aacaatcatg gcgggggcgt gcgcatgggc tatggcatgc
     6361 aggtgctcaa tagctacgtc catgacaatg gcattgtggg aatcgccggc ggaacgaatt
     6421 cgcccacagc gagcggcatc gtgattcaag gcaacacgat caatcacaac aactacgcca
     6481 aggggctttc ggactggggc gctggcggct tcaaggtcgg gcaaaccgcc aatctggtgt
     6541 ttcgcggcaa cacggtctcg aacaacgac
//
LOCUS       DASPUL010000299         3916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1830919, whole genome shotgun sequence.
ACCESSION   DASPUL010000299 DASPUL010000000
VERSION     DASPUL010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1830919"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VEJ00_07355"
     CDS             <1..367
                     /locus_tag="VEJ00_07355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX01013.1"
                     /translation="GTLISSLAWILLIVHPSVWMSVATLVVVAIGEITQQPRYYDYIS
                     RLAPADQQGTYMGFAFLPLGIGSFIAGKFSGWLMHHFGEELHNPAMIWWSVVAVGVAT
                     TLLLWIYDRLLRRESGPTA"
     gene            427..768
                     /locus_tag="VEJ00_07360"
     CDS             427..768
                     /locus_tag="VEJ00_07360"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HXX01014.1"
                     /translation="MPHYLQQVAYSREGWEALVANPQNRIEAVRPAIERLGGRIVQAW
                     FSFGDYDVVVISDLPDNVSAAAIAMAFAAGGACKSVQTTPLISPEEAVQALRKAGESG
                     YRPATVTARAA"
     gene            878..1363
                     /locus_tag="VEJ00_07365"
     CDS             878..1363
                     /locus_tag="VEJ00_07365"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HXX01015.1"
                     /translation="MRKFWTLAVTATIFSIFSTASAQSVNLLVGTWKLVSASSSTKQD
                     VYGAHPSGLLTYTLDGRMSVLIADDGRKPLSTDDRLTAPVEEKAGAFTTFVAYAGRYT
                     FTGKQVVHHVEIASIQNWVNTDLTRDATLDGDRLTLRGTTPRSRNGALQIFTLVWERV
                     K"
     gene            1381..2115
                     /locus_tag="VEJ00_07370"
     CDS             1381..2115
                     /locus_tag="VEJ00_07370"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HXX01016.1"
                     /translation="MPKQKTTPAEAAAGIRGALKNGGSAEHAAGVQWFFKEEIKSHGW
                     RTADLRRAVRRCRRDILRDHDFAFLVDVADQLFSSDVLEEKIAGVFLLEKLDAQCGDG
                     EFRMFERWLDRIRSWADHDGLVHYLIAPMVAAKPARVKSVFRWAKSRDRWHRRAACVA
                     LIRGTRKKMFLPEVKKLTSLLLHDEDDMVQKGLGWLLRETAKYDAKRTVPYLMKIRER
                     APRLVLRTACETLPKSARGKILTPHS"
     gene            2163..3329
                     /locus_tag="VEJ00_07375"
     CDS             2163..3329
                     /locus_tag="VEJ00_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXX01017.1"
                     /translation="MDLAAIQSALRERNIDAWLFYDHHHRDPIAYRVLGVPASLMVTR
                     RWFYLIPAEGEPVKLVHKIEAGHLDTLPGKKHQYSGWQELFDKLKAILANYRDIAMQY
                     SPNNIIFYVSLVDGGTIDLVRGLGKNVVSSADLVAQFESTWSEDQIRSHFAARDSIDA
                     IVAESFKEIGRRVRNGGTKEHEIQQWFMEAFRRESLLTDDPPIVAVNANAGNPHYEPG
                     PNHPTPIREGDVVLLDVWGKKNTPDAVYYDVTWMGFVGANPPERMRQIFEIVRDARDA
                     GVKTVVDAVGAGRRIAGWEVDRATRGHIKQKGYGDYFVHRTGHSIGTDVHSNGANMDD
                     LEIHDERQILPNSCFSIEPGVYLPEFGVRSEVNVLVRAKSAEVTGKIQREIVTI"
     gene            3360..3746
                     /locus_tag="VEJ00_07380"
     CDS             3360..3746
                     /locus_tag="VEJ00_07380"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HXX01018.1"
                     /translation="MASTDQVSLMSVLAPAVGWLIPGAGHMIQKRWIRGGLLFVSIVT
                     MFLLGLAMQGRIYHPNGGDILDILGFIGDLGAGGLYIVTLAMDWGQGVIAYATADYGT
                     KFMIVAGLLNFISVADAYHIAIGKKQ"
BASE COUNT          806 a         1137 c         1176 g          797 t
ORIGIN      
        1 gggaacactg atttcaagcc tggcctggat cttgctgatt gtgcacccgt cggtttggat
       61 gtcagtggcg acgctggtcg tcgttgccat aggagagatc acccagcaac cgcgttacta
      121 cgactacatt tctcgcctcg cgcctgccga tcagcaggga acgtacatgg gattcgcctt
      181 ccttccgctt gggatcggct ccttcattgc cggcaaattc agcggctggc tgatgcatca
      241 cttcggcgag gaactgcaca atcccgcgat gatctggtgg tcggtcgtgg cagtcggggt
      301 ggccacaaca ctcctgctgt ggatctacga ccgtttgctg cggcgcgaaa gcggcccaac
      361 agcttgactt cggcctgaac gcgtggaatc attcgcaccc agttagcgaa aacgtgagga
      421 ggctctatgc cacactatct gcagcaagtc gcgtattccc gcgaaggatg ggaggccctg
      481 gtcgcaaatc cgcagaatcg cattgaagcc gtgcggcctg cgatcgagag gctaggcggc
      541 cggattgtcc aggcctggtt ttcgtttggc gattacgatg tcgttgtcat ctccgattta
      601 ccggacaatg tgagcgccgc ggccattgcc atggcattcg ccgcgggcgg cgcctgcaag
      661 tcggtgcaga ccactcccct gatttctccg gaggaagcag tgcaggccct gagaaaggca
      721 ggagaatccg ggtatcgacc ggcgacagtc accgctcgcg cggcataggc gacagcttgg
      781 gtaaagaaac gaaggcggcc ttgctggccg cctttgtttt gaggaaatag tgttcctttt
      841 aacaacgaaa aaatcctccc tcgaggagtt ctatcgaatg cgaaagttct ggactctcgc
      901 ggtgaccgcg acgatcttct ccattttctc gaccgcatcc gcccagtcgg tgaatcttct
      961 agttggcacc tggaagctgg tttcggcctc ctcttcgacc aagcaggacg tgtacggtgc
     1021 tcatccctcg ggtctgctca catatacgct cgacggccgc atgagcgtgc tgattgccga
     1081 tgacgggcgc aagccgcttt ccaccgacga ccgtctgaca gctcccgttg aagaaaaagc
     1141 cggggccttc accacgtttg tcgcatacgc tggacgctac acctttaccg gcaagcaggt
     1201 ggtacatcac gtcgagatcg catcgatcca gaattgggtg aacactgact tgacacggga
     1261 tgcaaccctc gacggcgacc gtctgacctt gcgggggact accccgcggt caagaaatgg
     1321 cgcactgcag attttcaccc ttgtctggga acgagtgaag tagcggcagt gagggcaagg
     1381 atgcccaagc aaaaaaccac accggcagaa gcggcggcgg ggattcgggg tgcgctcaag
     1441 aacggcggct ccgccgagca cgctgctggc gtgcagtggt tcttcaagga agagatcaag
     1501 tcgcatggct ggcgcaccgc cgatttgcgg cgcgcggtgc ggcgctgccg ccgtgacatc
     1561 ctgagagacc atgacttcgc gtttctagta gacgtggccg accaactgtt ctccagcgat
     1621 gtactcgaag agaagattgc tggcgtcttc ttgctcgaga agctcgacgc acaatgcggc
     1681 gacggtgagt tccgcatgtt cgagcgctgg cttgaccgca tccgcagctg ggccgatcat
     1741 gatgggctgg tgcactacct gatcgcgccc atggtggcgg cgaagcccgc ccgagtaaag
     1801 tctgtatttc gctgggcaaa gtcgcgggac cgctggcatc ggcgggcggc gtgcgtcgcg
     1861 ctcatccggg gaacacggaa gaagatgttt ctgccagagg tcaagaaact cactagcttg
     1921 cttttgcatg acgaagacga catggtccag aaaggcctgg gctggctgct ccgtgagaca
     1981 gcaaagtacg acgccaaacg caccgtgcca tatcttatga agatccgcga acgcgctccg
     2041 cggctggtgc tgcgaacagc gtgtgagacg ctgcccaagt cagctcgagg gaagatactc
     2101 actcctcact cctaacctga ttcgcccaaa tccctgctct tgtttacaat agaagtttgc
     2161 ccatggacct tgctgccatc cagtctgcgc tgcgcgaacg caatatcgac gcctggcttt
     2221 tttacgatca ccaccaccgc gatcccatcg cctaccgcgt cctgggcgtt cccgcgagcc
     2281 tgatggtcac ccggcgctgg ttctacctga ttccggccga aggcgagccg gtaaagctgg
     2341 tgcacaagat cgaagccggt catctcgaca cgctgcctgg caaaaagcac caatattccg
     2401 gctggcagga gttgttcgac aagttgaagg cgatcctggc caattaccgc gatatcgcca
     2461 tgcagtactc gccgaacaac atcatctttt acgtttccct ggtggatggc ggcacgatcg
     2521 atctcgtacg cgggctgggg aagaacgtgg tgagctcggc cgacctcgtg gcgcaattcg
     2581 aatcgacctg gagcgaggat cagatccgct cgcactttgc cgcgcgcgac agcatcgacg
     2641 cgatcgttgc cgaatccttc aaggaaatag gacgccgggt tcgcaacgga ggcacgaaag
     2701 aacacgagat ccagcagtgg ttcatggaag cattccgccg cgagagcctc cttaccgacg
     2761 acccgccgat cgtggccgtc aacgcgaatg ccggcaaccc gcattatgag cccggtccca
     2821 accatcccac gccgattcgc gaaggcgatg tcgtactact cgatgtgtgg ggcaagaaga
     2881 acactcctga cgccgtgtac tacgatgtga cctggatggg ctttgtgggc gcgaacccgc
     2941 cggagcgcat gcgacagatt ttcgagatcg tgcgcgacgc gcgcgacgcc ggcgtgaaga
     3001 cggtggtcga tgcggtcggt gccggccgcc gcattgccgg atgggaagtc gaccgggcca
     3061 cccgcgggca catcaagcag aaaggctacg gagattattt cgtccaccgc acgggacatt
     3121 ccatcggcac agacgtccat tccaatggcg ccaatatgga tgatcttgaa attcacgacg
     3181 agcggcaaat cctccccaac tcctgcttct cgatcgagcc gggcgtttat ctgcctgagt
     3241 ttggcgtgcg cagcgaggtc aacgtgctgg tgcgggccaa gtcggccgaa gtgaccggga
     3301 aaattcagcg cgagattgtg accatctgaa tggcaaattc caccacagag aaagctaaag
     3361 tggcatccac agatcaggtg agcctgatga gcgtgctcgc gcctgccgtg ggttggctga
     3421 tcccgggcgc aggccacatg atccagaagc gctggatccg cggcggcctg ctgttcgttt
     3481 cgattgtaac gatgtttctg ctcggactcg ccatgcaggg ccgcatctat cacccgaacg
     3541 gcggggacat cctggatatt ctcggcttca tcggtgatct gggcgcaggc ggtttgtaca
     3601 ttgtgaccct ggccatggat tggggtcagg gcgtcatcgc ctacgcgaca gcagactacg
     3661 gaaccaagtt tatgatcgtg gccggattgc tcaatttcat ctccgtcgcg gatgcgtatc
     3721 acattgcgat tgggaagaag cagtgagtcg caggcagcga ccggtcaccg gttaaaatct
     3781 cttttcggat aagaaggcag tgaccataac tctcagtcat ttcagcgcgg cggtggtatt
     3841 tgccctgttt gcgtcgatcg tgttcgggat cacccaccgc gagagcacgc gcgacatggt
     3901 gcgctacgga gtctac
//
LOCUS       DASPUL010000300         9537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1967643, whole genome shotgun sequence.
ACCESSION   DASPUL010000300 DASPUL010000000
VERSION     DASPUL010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9537)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9537
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967643"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VEJ00_07385"
     CDS             <1..548
                     /locus_tag="VEJ00_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01019.1"
                     /translation="LLFGLATLSLSLFHMTRFDLDIDFRTVAAARVVQAVGMAFLFVP
                     INTAAYAFLPRDKNNAASGLMNLARNIGGSVGISVVTTMLDRRTQFHLNQLANHLSGA
                     SLAVQARLQGLTQAMRGHGASAAFAKQQAYALIQGTAIRQATMLAYIDCFWLLGVAIL
                     LMVPMVFLIKKARPGGGMAVH"
     gene            558..1223
                     /locus_tag="VEJ00_07390"
     CDS             558..1223
                     /locus_tag="VEJ00_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HXX01020.1"
                     /translation="MFRLVTIEREYGCGAGGIASKLAEHLGWKLWDHRLTEEIARLAH
                     VDPSAVRRCDERVDSRLHRLAKSFWRGSYERSAALGGQIFDTDRMMSMMQDIMNTIAA
                     EGNAVVVGRGAPFFLRQNPETFHVFLFAPRAEKIRRTVADGHSEEEAEELVDSVDRER
                     MAYVKHYFDADWPTRSLYHLMLNTAVGDGPVVQTILNTMRLLEGAPKATDYEPPRVPA
                     SIR"
     gene            complement(1187..2269)
                     /locus_tag="VEJ00_07395"
     CDS             complement(1187..2269)
                     /locus_tag="VEJ00_07395"
                     /inference="COORDINATES: protein motif:HMM:NF000689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HXX01021.1"
                     /translation="MTYDAILVVSFGGPESREDVIPFLENVLRGRNVPRERLLAVAEH
                     YYHFEGRSPINQQTRDLISALETELARHGPKLNVYWGNRNWHPMLADTLRQMNKDGVR
                     HALAFVTSAYSSYSGCRQYREDIARAQSEVGPGAPQIDKLRAFFNHPGFIAATEDRMR
                     DALEQVPVNARHNVQVIYTAHSIPVSMANTCDYVRQLEEVRALVSARLAITSDALVYQ
                     SRSGAPGQPWLEPDILDYLREVKARNVASAVVLAPISFISDHMEVLYDLDVEARQLCD
                     SLGLPMVRATTVGVHPKFVAMIRELIEERTTPGSECPALGSLGPRPDVCAEDCCPAPQ
                     RPAQVHPTGEKSGTRQRIDAGTRGGS"
     gene            complement(2415..3773)
                     /locus_tag="VEJ00_07400"
     CDS             complement(2415..3773)
                     /locus_tag="VEJ00_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01022.1"
                     /translation="MQNAPLAIRPILRENNFPLMNNPGRVRSANAKNLCRVKIVTAAL
                     VGVAVSAAPAIASASGEPSPGEHTSADSGLYVKVQLAHPLKLSRLKTGDEVDGSLLRD
                     VYSSDRQLFAAGSRVRLTVDHLEKRPRIPNDHWPGVVRLFTPRRQKYPVFKTASVTQG
                     QTERSLAVSLISVSRVREVHAQAKKARPGQPDEKPGAVETSGGSKGKIVAPTVVLEAS
                     IEDSAQASDQSAAGTGWPGASGPQTLPIGTRFKILLLGEVSASKSKPGDVVRARLLEP
                     VMLNSQLAVPAGTLLDGRVVHRTPPRWLSRSGSLYLAFTEITLPQGNRFPITASVAGA
                     ELDQRSHTRIDSEGQLHGERPGKAWMAINIGVTAGLAKEADDSVQLVIEAIVSTATDA
                     STAGTARIVSSCVSGLYMATRRGRDVVLPRFTEIDISLDRPLSLGGSSRPIASSSAVG
                     EQ"
     gene            complement(3790..3866)
                     /locus_tag="VEJ00_07405"
     tRNA            complement(3790..3866)
                     /locus_tag="VEJ00_07405"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3830..3832),aa:Arg,seq:ccg)
     gene            complement(3999..4313)
                     /locus_tag="VEJ00_07410"
     CDS             complement(3999..4313)
                     /locus_tag="VEJ00_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXX01023.1"
                     /translation="MKNIFVGNLSFNTSDDELRQAFEPYGQVDRVSIMTDRDTGRSRG
                     FGFVEMANNEDGEKAIAALNGAQLGGRTINVNEARPKPERAASGGRGDRGGRRGGGRD
                     RW"
     gene            complement(4551..4808)
                     /locus_tag="VEJ00_07415"
     CDS             complement(4551..4808)
                     /locus_tag="VEJ00_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01024.1"
                     /translation="MKTRPDTRKRQTATHSGTKKPALLSMFLVPCSCGTTFAVAGDYD
                     QHGTAWSRYLTCPNCGKRHDPRNRLLQMGFHPEGYWKVDQC"
     gene            complement(4805..6625)
                     /gene="typA"
                     /locus_tag="VEJ00_07420"
     CDS             complement(4805..6625)
                     /gene="typA"
                     /locus_tag="VEJ00_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HXX01025.1"
                     /translation="MSSGIRNIAIIAHVDHGKTTLVDAMLKQSGTFRANEAVAERVMD
                     SNELERERGITILAKNTAIFYHDVKINIVDTPGHSDFGGEVERALKMVDGVMLLVDAS
                     EGPLPQTRYVLGKALEAHLPPIVVINKIDRADARPQEVLNEIYDLFIDLDAKEEQLDF
                     PVLYTNAKTGTASKDVRGGSDLRPLFDAILQTIPPPKGEPTGPLQILVANLDYSDYLG
                     RLAIARVFNGTMRTGEDVAIAKLDGSLHKTRITKLFSFSGLKRTDITETELGDIIAVA
                     GVEGITIGETITNVENSAPLPPIAIDEPTIAMLFTINTSPFAGKDGTYVTSRNLRERL
                     EKELLTNVSLRVEETGTTDSFKVLGRGELQLSILIEMMRREGYELMVGKPEIVTKRID
                     GKLMEPVEHLTVDVPEEFVGVVMEQLGARKGEVTNMHNHGYGRVRIEFRVPSRGLIGL
                     RSQLLTDTRGTIVMNALFDGYTEWQGEIPHRLTGALIADRPGVSTAYALWGLQERGEL
                     FVGPGVELYEGMIIGENAKDTDLDVNAVREKKLTNMRASTADEAIRLVPHRPLNLEQA
                     IEFIADDEFVEITPKALRLRKKVLQANKRKKSALATVEES"
     gene            6855..9002
                     /gene="dcp"
                     /locus_tag="VEJ00_07425"
     CDS             6855..9002
                     /gene="dcp"
                     /locus_tag="VEJ00_07425"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: protein motif:HMM:NF007624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HXX01026.1"
                     /translation="MKTLLRMGAFMVSGILAVGSGTVAAGGNGQFGPSNPFYAPSALP
                     LQAPPFDKIKDSDYQPAIEAGMAQQREEVRAIADNLDAPTFDNTIVALEKSGKLLNRV
                     MAVFTGVTGANLNPTLEKLQEIEAPKLAAHQDAIYLDPKLFGRVEAIYKNREVLKLDP
                     EALRLVEYDYEQFVHAGAKLSDSDKAELKKLNEEESTLSTAFTTKLLAATKAGAYVTK
                     DRSALAGLSPARLEGAAQAATDRKVDGYVIPLQNTTQQPDLASLSQRSTRQTIFENSW
                     NRAEHGDANDTRDIVARLAQLRAQKAKLLGFPDYAAWKLEDQMAKNPEAAFKFMDALA
                     PGATAKAREEAKDIQALIDAQKGGFTLQPWDWNFYSEQVRKAKYDLDEEQIKPYFELN
                     NVLQNGVFYAANQLYGLTFKERTDIPVYHPDVRVFDVFDADGKQLALWYCDYFKRDNK
                     NGGAWMDVFVNQSKLLGTMPVVFNVANFSKPAPGQPALLTFSDVTTMFHEFGHALHGM
                     FANTVYPSLSGTQVARDFVEFPSQFNEHWATYPAVFEHYAKHYETGAPMPSDLAAKVK
                     KAEKFNQGYAITEVVVAAQLDMLWHTLPSNAPLETPDDFEIKALRSKQLWISEVPSRY
                     RSSYFAHIWGGGYSAGYYAYLWSEMLDDAAYQWFEDHGGMTRANGDRFRQMVLSRGNT
                     QELGKMYAAWFGGEPGITPMLKYRGLLPEEKGK"
     gene            complement(9048..>9537)
                     /locus_tag="VEJ00_07430"
     CDS             complement(9048..>9537)
                     /locus_tag="VEJ00_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01027.1"
                     /translation="LALSGLRSLMARLAPRYSAAAVSTLVFVIAIPYTIAYRSTDFGP
                     IRQDSGLADFNQLCQAVRDDTSPHDVLIYFRARALSLYTNRPGSTYNYHGTEQELWQY
                     ARDIHATHLITTKAFHDDEGFLDRYVEEYSSSLELTYENPHFRLYRIRSQTNAQNLPT
                     PH"
BASE COUNT         1899 a         2783 c         2927 g         1928 t
ORIGIN      
        1 tgctgctgtt tggtctggcg accttgtctt tgtcgctgtt tcacatgacc aggttcgatc
       61 tcgacatcga cttccgaact gtggccgcgg cgcgggtggt gcaagccgtc gggatggcat
      121 ttcttttcgt ccccatcaac acggcggcgt atgcgttcct gccgcgcgac aagaacaatg
      181 cggcttcggg cttgatgaac ctggcgcgca acatcggcgg cagcgtaggg atttcggtcg
      241 tcacgaccat gctggacagg cgcactcagt tccacctgaa ccagttggcg aatcatctca
      301 gcggagccag ccttgccgtg caggccagat tgcagggcct gacacaggcc atgcgcgggc
      361 acggggcaag cgccgcattt gccaaacagc aggcctatgc gcttatccag ggaactgcta
      421 tccgccaggc cacgatgctt gcctacatcg attgtttttg gcttcttggc gtggccattc
      481 tgctgatggt gcccatggtg ttcctgatca aaaaagccag gccgggcggc gggatggcgg
      541 tacactagag ttaacgcatg tttcgtctcg tcaccattga gcgcgagtac ggctgcggcg
      601 ccggcggaat tgcctcgaaa ctcgccgagc atctgggatg gaagctctgg gaccaccggc
      661 tcacagaaga gattgcacgg cttgcccatg tcgacccctc agccgtgcgg cgctgtgatg
      721 agcgcgtgga cagccgctta catcgcctgg ccaaatcctt ctggcgtggc agttacgaac
      781 gcagcgcggc tctgggtggg cagattttcg ataccgaccg catgatgtca atgatgcagg
      841 acatcatgaa cacgatcgcg gccgagggaa atgccgtggt ggtcggacgg ggggcgccgt
      901 ttttcctgcg gcagaatccg gagacattcc acgttttcct gtttgctccg cgggccgaga
      961 aaatccgccg cacagtcgcg gatggacaca gtgaagaaga agccgaagaa ctggtggatt
     1021 ccgtcgaccg agagcgcatg gcctacgtga agcactactt cgacgcggac tggccgacgc
     1081 gctcgctcta ccatctgatg ctgaataccg ctgtgggcga tgggccggtg gttcagacca
     1141 tcctcaacac catgcgactg ctcgagggtg caccgaaagc cacggactac gagccgcccc
     1201 gagttccagc gtcaatccgc tgacgcgttc ccgatttttc accagtgggg tgcacctgag
     1261 caggccgttg cggcgcaggg caacaatcct ccgcacagac atccggccgc ggcccgagtg
     1321 atcccaacgc agggcattcc gatcctggcg tcgtcctctc ttcaatcagt tcgcgaatca
     1381 tcgcgacgaa cttgggatgc acgccgaccg ttgtcgcgcg aaccatgggc agtccgagcg
     1441 agtcgcagag ttggcgcgcc tcaacatcca ggtcgtacag cacttccata tgatcagaaa
     1501 tgaagctgat cggcgcgagc acgaccgcgc tggccacatt gcgtgccttt acctcgcgca
     1561 ggtagtcgag gatatctggc tccagccagg gctgaccggg cgcgccactg cgactctgat
     1621 aaaccaatgc atcgctcgtg atggcgaggc gcgcagaaac gagcgcgcgg acctcctcga
     1681 gttgacgaac gtagtcgcac gtattggcca tcgaaaccgg gatgctgtgc gccgtgtaga
     1741 tgacttgcac gttatgccgg gcgtttacgg gaacctgctc cagtgcatcg cgcatgcggt
     1801 cttcggtggc agcgatgaat ccggggtggt tgaagaaggc gcggagcttg tcgatttgtg
     1861 gagcgccggg cccaacctcg ctctgagccc gggcaatgtc ttcgcgatat tgccggcatc
     1921 cggagtaaga actgtacgcg gaagtgacga aggccagggc atggcgaacg ccgtccttgt
     1981 tcatctggcg aagggtgtcg gccagcatag ggtgccagtt gcggttcccc cagtagacgt
     2041 tcaacttcgg accgtgtcga gcgagttcag tttccagcgc cgagatcaaa tcccgggttt
     2101 gctggttgat gggactcctg ccttcgaagt ggtagtagtg ctcagcgacc gccaacaggc
     2161 gttcccgagg aacatttctt ccgcgcagca cgttttcgag gaagggaatg acatcttcgc
     2221 gggattccgg accgccgaag gaaacgacca ggatggcgtc gtaggtcatt gcttttatgc
     2281 gccgagagcg atgttttcgc ttggcggcag agccagcaga tatgttatac ggtccccttg
     2341 ccgggcagat agccgtgggc tagcgaggcg gaaagggacg ggatgctgcc ggcagagttc
     2401 ctgattctgt ttctctactg ctcaccaacc gcggagctgg aagcaatggg tcgcgacgag
     2461 ccaccgagcg acagtgggcg atccaacgag atgtcgatct cggtgaagcg cggcagaacc
     2521 acatccctcc cccgccgggt tgccatgtac aggccagaga cacagctgga tacgatacga
     2581 gccgttcctg ccgtggatgc atccgtagca gtcgacacga tcgcctcgat gacaagctgc
     2641 acggagtcat ctgcttcctt tgccaaaccg gccgtgacgc cgatattgat ggccatccac
     2701 gcctttccgg gccgctcgcc atgaagttgg ccctcggaat caatccgcgt gtgtgaccgc
     2761 tggtccaatt ctgctccggc caccgaagcc gtaatgggga agcgattgcc ctgcggcaga
     2821 gtaatctcgg tgaatgcgag gtacaacgag cccgaacggc tcaaccagcg tggcggcgtg
     2881 cggtgcacta cccggccgtc gagcaaagtt cccgcaggca cggccagctg tgaattgagc
     2941 atcacgggct cgagcaggcg ggctcgaacc acgtcgccgg gcttgctctt cgatgcgctg
     3001 acctccccca gcagaagaat cttgaaccgc gttccgatcg gaagggtttg cgggcccgag
     3061 gcgcccggcc atcccgttcc ggcggcgctc tgatcggaag cctgagccga gtcctcaatc
     3121 gaggcttcca gcaccacggt cggtgccaca atctttcctt tgctgcctcc gcttgtctcg
     3181 actgcccccg gtttttcatc tggctggccg ggcctggctt tcttcgcctg tgcgtgcact
     3241 tcgcgaaccc gactcactga aatcagtgaa actgccagac ttctttctgt ttggccctgc
     3301 gtgacgctgg cagttttgaa gacgggatac ttctggcgcc ggggtgtgaa cagcctgacg
     3361 acgccgggcc agtgatcatt cggtatgcgc ggtctctttt ccagatgatc gaccgtcagc
     3421 cgcacgcggc taccggcggc gaacagctgg cggtcggacg aatagacatc tcgcaacaag
     3481 ctgccatcga cttcatcccc ggttttcagc ctggagagct tcagcggatg ggcgagttgc
     3541 accttgacgt agaggcccga gtcagcggac gtgtgttcgc caggggaggg ttcaccactg
     3601 gccgaggcga tagcgggagc tgccgagact gccacgccta ccaaggctgc cgtgacgatc
     3661 ttgacccgac agagattctt ggcgttcgcg ctccgaaccc tccccgggtt attcatcaac
     3721 gggaagttgt tctcacgaag gatgggtcgg attgccaggg gcgcgttttg cattcactgc
     3781 tgagtgatct ggtgcgcccg gagagattcg aactcccggc ctgttgctcc ggaggcaacc
     3841 gctctatcca gctgagctac gggcgcacac cgatagttag agtctacatg cgaggagagc
     3901 gagcttcaat ggcaggcgtg cagggggacg ctgccgaagc ctcgtctaac cgaagtcgga
     3961 gggtgcgatc cagcccaact ttcagatcct gaaatcgttt accagcggtc gcgcccgccg
     4021 ccacgccgcc caccgcgatc cccgcgtccg ccgctggcgg cgcgctcggg tttaggccgg
     4081 gcttcattga cgttgatggt gcgtccgccg agttgcgctc cgttcagagc cgcgatcgcc
     4141 ttctcgccat cttcgttgtt tgccatctcg acaaagccga agccgcgcga ccgaccggtg
     4201 tcgcggtcgg tcatgatgct gacgcggtct acttgcccat agggctcaaa agcctgccgc
     4261 aattcgtcat cgctggtgtt gaaactgaga ttccccacga aaatgttctt caagcatctg
     4321 cccctgcatt gagctgtctg atgtgcgagc tcgatgcggc agactggcag gtcggagagc
     4381 cgttcctgaa aatcgttgcc ggagctggcg aacagtcgaa tgcgcgcagc agtatagcag
     4441 gtttacgtga agctgctgtc aaatcgcgag tcgggctcca gcttcacagc gaaggacgcg
     4501 aatggcacaa tgggaagtcg gcaagggcga ggcgataagc gggattccag tcagcactga
     4561 tcgaccttcc aatatccttc tggatggaat cccatctgca gcagccggtt tctggggtcg
     4621 tgtcgcttgc cgcagtttgg acaggtcagg tagcgactcc atgccgtgcc gtgctggtcg
     4681 tagtctccgg ctacggcgaa ggttgtgccg caactgcagg ggacgaggaa catgctaagg
     4741 agcgcaggtt tcttggtgcc actatgggtt gcggtctggc gcttccgggt gtcgggtcgt
     4801 gtcttcacga ctcttccact gtggcgaggg cactcttttt acgcttgttc gcctgcagga
     4861 ctttcttgcg aaggcgcagc gctttgggtg taatttccac gaattcatca tcggcaatga
     4921 actcgatcgc ctgctccaga ttcaacgggc ggtgcggaac caggcggatc gcctcgtcag
     4981 ccgtggaagc acgcatgttg gtgagcttct tctcacgcac ggcattcacg tcgaggtccg
     5041 tgtctttggc gttttcaccg atgatcattc cttcgtatag ttcgactccc ggcccgacga
     5101 atagctcacc gcgctcctgc aggccccaga gcgcgtaggc ggtcgagacc ccggggcgat
     5161 cggcgatgag cgcgcctgta agccggtgcg gaatttcgcc ctgccactcg gtatagccat
     5221 cgaacagcgc attcatcacg atggtgccgc gcgtgtcggt gaggagctgg ctgcgcagtc
     5281 cgatcagccc gcgactgggg acacggaatt caatgcgtac gcgcccgtag ccgtgattgt
     5341 gcatgttcgt gacttcgccc ttgcgggcgc ccagttgctc catcaccacg ccgacgaatt
     5401 cttccgggac atcgactgtg aggtgctcga ccggctccat cagcttgcca tcgatccttt
     5461 ttgtgacgat ctccggcttg ccgaccatca gctcgtagcc ctcgcggcgc atcatctcga
     5521 tgagaatcga aagctgcagc tcaccccggc cgagaacttt gaacgaatca gtcgtgcctg
     5581 tctcttcgac gcgcagcgaa acgttcgtga gcagctcctt ttccagccgc tcgcgcaagt
     5641 tgcgggaagt gacataggtg ccgtccttgc cggcgaacgg cgaggtgttg atcgtgaaca
     5701 gcatggcgat ggtgggctca tcgatcgcga tcggcggcag tggagcagaa ttctccacat
     5761 tcgtgattgt ttcgccgatg gtgatgccct ccacccccgc caccgcgatg atgtcgccca
     5821 attccgtttc agtgatgtcg gtccgcttca gaccggaaaa agagaacagc ttcgtgatcc
     5881 tggttttgtg cagcgagccg tcgagcttgg cgatggccac gtcttccccc gtgcgcatgg
     5941 tgccgttaaa aacgcgcgcg atcgccagcc gtcccaggta atcactgtag tcgagatttg
     6001 cgaccagaat ctgcaggggc cctgtcggct cgcctttggg gggcgggatc gtttgcagaa
     6061 ttgcatcaaa caggggacgc agatcgcttc cgcccctcac atccttcgat gccgtgccgg
     6121 tcttggcatt ggtgtaaagg accgggaagt cgagttgctc ttccttggcg tcgaggtcga
     6181 tgaagagatc atagatctcg ttgaggacct cttgcgggcg ggcgtcggca cgatcaatct
     6241 tgttaatcac gacgatgggc ggcagatgag cttccaaagc cttgccgagc acgtagcggg
     6301 tctggggcaa cgggccttcg ctggcatcga ccagcaacat cacgccgtcc accattttca
     6361 gtgcgcgctc gacctcgccg ccgaaatcgc tgtggccggg cgtgtcgacg atgttgatct
     6421 tcacgtcgtg atagaagatg gccgtgttct tggccagaat cgtgatgccg cgctcgcgct
     6481 ccagttcgtt cgagtccatc acgcgttcgg ccacagcctc gttggcgcgg aatgtcccgc
     6541 tctgcttgag catggcgtcg accagcgtgg tcttgccgtg gtcgacgtgc gcgatgatgg
     6601 caatgttgcg aatccctgaa ctcaacggaa atccttcccg gcgttgcctc ttaccaatgt
     6661 actatgcggg cacgaaatcg gcgatggaag acgatgcggc gcaccgtagc gtgagctcgc
     6721 ccgcatctgc ggacgacatt cgcaacggtt cttatgccga caactgcctg ccgactacaa
     6781 acggagcgac gcgtccgtta caatcaagcg cgtggcaaag ccatcaaaca cgaggcaatc
     6841 aggacgggca cgaaatgaag acacttctaa ggatgggagc tttcatggtg agtggaatac
     6901 tggcggttgg gtcgggcaca gtggcagcgg gaggcaatgg ccagttcgga ccgagcaatc
     6961 cgttttatgc tccaagtgcg ttgccgctgc aggcgccgcc cttcgacaag atcaaggaca
     7021 gcgactatca acccgcaatt gaagcaggca tggcccagca gcgcgaggaa gtgcgggcga
     7081 tcgccgacaa cctcgacgct cccacattcg acaacacgat tgtggcgctc gaaaagtcgg
     7141 ggaagctgct caaccgcgtg atggccgtct ttaccggagt caccggggct aacctcaatc
     7201 cgacgctgga gaaactgcag gaaatcgagg cccccaaact ggccgcgcat caggacgcaa
     7261 tctacctgga tcccaagctt ttcggccgtg tggaggcgat ttacaagaat cgcgaagtgc
     7321 tgaaactcga tccggaagcg ctgcgacttg tcgagtacga ctacgagcaa tttgtgcatg
     7381 ccggggccaa gctttctgac tccgacaagg ctgagctgaa aaaactgaat gaggaagagt
     7441 cgacgctatc gacagcgttc acgaccaaat tgctggccgc gaccaaggcc ggtgcctacg
     7501 tcacgaagga caggagcgca ctggccgggt tgagccccgc gcgcctggaa ggggccgcgc
     7561 aggctgcaac ggatcgcaag gtcgacggct acgtcatacc gctgcaaaat acgactcaac
     7621 agccggacct ggcttcgctg agccagcggt cgacacggca gaccattttc gaaaattcgt
     7681 ggaaccgtgc cgaacacggg gatgcgaacg acacgcgcga catcgttgca cgcctggcgc
     7741 agctgcgcgc gcagaaggcc aagctgctcg gtttccccga ctacgccgcc tggaaactgg
     7801 aagatcagat ggcgaagaac cccgaagctg cgttcaagtt catggatgcc ctggcgcccg
     7861 gcgcaaccgc caaggcaaga gaagaagcca aggatatcca ggcgctgatc gatgcccaga
     7921 agggcggctt cacgctgcag ccctgggatt ggaatttcta ttccgaacaa gtccgcaaag
     7981 caaagtacga cctggatgaa gagcagatca aaccgtactt cgagctgaat aacgtattgc
     8041 aaaacggagt cttctacgct gcgaatcagc tctatggcct caccttcaaa gagaggaccg
     8101 acatccccgt ctatcacccg gacgtgcgag tgttcgacgt gttcgacgcc gacggcaagc
     8161 agctggcgct gtggtattgc gactacttca agcgcgacaa caagaacggc ggcgcctgga
     8221 tggacgtttt tgtaaaccag tcgaagttgc tgggcacgat gccggtggtc ttcaacgtcg
     8281 ccaatttcag caagcctgcg ccaggccagc ctgctctgct caccttcagc gacgtcacta
     8341 cgatgttcca cgaatttggc cacgcgctgc acgggatgtt cgccaacacg gtgtatccaa
     8401 gcttgtcggg cacgcaggtg gcgcgtgatt tcgtggaatt tccgtctcaa ttcaacgagc
     8461 actgggcgac gtatccggcg gtgttcgagc actatgccaa gcactacgag acgggagcac
     8521 ccatgccctc cgacctggcg gccaaggtga agaaagcaga gaaattcaac cagggctatg
     8581 ccatcacgga agtcgtagtg gcggcgcagc tcgatatgct ctggcatacc ttaccctcga
     8641 atgcgcccct tgaaacacca gacgattttg aaataaaggc tctcaggagc aagcaactct
     8701 ggatcagcga ggtgccctcg cgctaccgct ccagctattt cgcgcacatc tggggtggcg
     8761 ggtattcggc cgggtactac gcctacttgt ggagcgagat gctggacgat gctgcctacc
     8821 agtggttcga agatcacggc ggcatgacgc gggccaacgg cgaccgattc cgccagatgg
     8881 tcttgtcccg gggaaacacc caggaactgg ggaagatgta tgcggcgtgg ttcggaggag
     8941 aacccggcat tactccaatg ttgaagtatc gcggcctgct gccggaggaa aaaggcaaat
     9001 agaccggctg gttccgccag acttgagatg gagttaagct gccttcttca gtgtggcgtg
     9061 ggcaggtttt gcgcattagt ttgcgagcgg attcggtaca gcctgaaatg cggattctcg
     9121 taggtaagct cgaggctgga cgaatactcc tcgacgtagc ggtcgaggaa gccctcgtca
     9181 tcgtggaagg ccttggtcgt gatcagatgg gtggcgtgaa tgtcgcgcgc gtactgccac
     9241 agttcctgtt ctgtcccgtg ataattgtag gtcgatccgg ggcggttcgt gtacagcgat
     9301 agagcccgtg cacggaaata aatcagcaca tcgtgaggcg atgtgtcgtc gcgcacagct
     9361 tgacatagct gattgaagtc ggcgaggccg ctgtcttgac gaatcggacc gaagtctgtg
     9421 ctgcgatatg cgattgtgta gggaattgca atgacaaaca ccagcgtact gactgcggcg
     9481 gccgaatagc gcggcgccag cctcgccatc aggctgcgca gcccggacag cgccaga
//
LOCUS       DASPUL010000301         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1554812, whole genome shotgun sequence.
ACCESSION   DASPUL010000301 DASPUL010000000
VERSION     DASPUL010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554812"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..899)
                     /gene="ribA"
                     /locus_tag="VEJ00_07435"
     CDS             complement(276..899)
                     /gene="ribA"
                     /locus_tag="VEJ00_07435"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HXX01028.1"
                     /translation="MSPDPVRQIASADFPTRWGNFRIYGFSGEFNDGDGSGPRVEEAV
                     ALVMGDIHAKPALVRVHSQCLTGDVFHSLRCDCRQQLELSLSMIAREGAGILIYEQQE
                     GRGIGLMAKLRAYELQDSGLDTVEANERLGFKADHRDFALPAGILLALGVTSVRLLSN
                     NPDKVEALERAGVRVAERVPCEVTPSPQAEEYLKTKKEKLGHIFTSS"
     gene            complement(1135..1833)
                     /locus_tag="VEJ00_07440"
     CDS             complement(1135..1833)
                     /locus_tag="VEJ00_07440"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HXX01029.1"
                     /translation="MKNCIRATFVLALLCTALSTASFASSMYIVQGIAGRNFATVSDP
                     AFPVDILINDEACYARGIAFGTIVGPLTFLPGTYSVKVSIADSLAPCSNTPLIDNSVK
                     IGAKTDVSAVLTLDADNTPTLLTFTNNLSPVTPNLTRVLFALGANSAAVDVVFENTDT
                     KKVSTFPVKAGGFLDVNLAAGAYTVAINEGTTQLVGPTTLFLSSQSVDLLYAVGQASN
                     NTVVLEMKTLRDVI"
     gene            complement(2060..>2302)
                     /locus_tag="VEJ00_07445"
     CDS             complement(2060..>2302)
                     /locus_tag="VEJ00_07445"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXX01030.1"
                     /translation="TSDPVVYPWQLPEAAGNEVIEITIDESGQITRKIVLQSLGPEID
                     SRCLAALDNWRFHPATHNGSPIASKQDAIFPFKARG"
BASE COUNT          490 a          616 c          743 g          453 t
ORIGIN      
        1 gagatgagtc agcgaattag agcacggcgc gagacttcag gcaaagtggg gcaggcaaat
       61 catgaggcag ttcgtagacg agccctgcga tcaaaagcgt tgaccccggc agggcagaaa
      121 acgcgaagaa aaagttccag aacatgttcc ttgaggtttt ttcgcgatct ttgcggcctt
      181 tccttcgcgc gctttgcagt aagggctttt gaccgcggag atcgctgagg aaaagccgca
      241 gagatcgcaa agaaaagctg agggcccgat agcgtctaac tggaggtgaa gatgtgtccc
      301 agcttttcct tcttcgtctt cagatattcc tctgcttgcg ggctgggcgt aacttcgcag
      361 gggacgcgct cggccacgcg cactccggcg cgctcgaggg cttcgacctt gtcgggattg
      421 ttcgagagca ggcgcacgct ggtcactccg agcgccagca gaatgcccgc gggcagcgcg
      481 aagtcgcggt ggtcggcctt gaagcccagg cgctcgttgg cttcgacggt gtcaaggccg
      541 gagtcctgca gttcgtaagc gcggagtttg gccatcaggc cgatgccgcg tccttcctgc
      601 tgctcgtaga tcaggatgcc ggcgccttcg cgggcgatca tcgacaggga aagctcaagc
      661 tgctggcggc aatcgcaacg cagggagtgg aagacgtcgc cggtgaggca ctgagagtgc
      721 acgcgcacca gcgcgggttt agcgtgaatg tctcccatga ccagagcgac ggcttcttcg
      781 acgcgggggc cggagccatc gccgtcattg aactcgccgc tgaagccgta gatgcggaag
      841 tttccccagc gcgtggggaa atcggccgaa gcgatttggc gaaccgggtc aggactcaca
      901 ttttgattat aacggggcgg gacttggctt tggctcggcg aaggtgcggt ttgtgacgtt
      961 ttcgagctta atagtacgga gatcccgatt tttcgaaaaa cagcttaacc gcaaagttcg
     1021 ccgagaacgg ccgcaaagaa cgcagagaac aacaactgct ggaatcactt gcctttgaag
     1081 agttaacgga gcaattaatc cgaaaaaggg ccgcccaagc tacggcccct cgggctagat
     1141 tacatctcgc agcgttttca tctcgagcac gacggtgttg ttgcttgcct ggccgacggc
     1201 gtagagcaga tccaccgact gcgaagacaa gaacagcgtg gtcggtccca ccagctgagt
     1261 cgtaccctcg ttgatggcca cggtgtaggc gccagcggcg agattgacgt cgaggaaccc
     1321 gcccgcctta accgggaacg tagacacctt cttggtatcg gtgttctcga agacaacgtc
     1381 gaccgcggcc gaattcgccc ccagcgcaaa cagcacgcgg gtgaggttcg gagtcacggg
     1441 cgacaggttg ttggtgaacg tcagcagggt tggcgtgtta tcggcatcga gcgtgaggac
     1501 tgccgagacg tcggtcttgg caccgatctt caccgagttg tcgatcaacg gagtgttgct
     1561 gcagggcgcc agcgagtcgg cgatgctgac cttcacgctg taagttccgg gaaggaaggt
     1621 gagcgggccg acgatggttc cgaaggcaat gccgcgcgcg tagcaggcct cgtcattgat
     1681 cagaatgtcc acgggaaagg cgggatctga aaccgtggcg aaatttcgtc cggcaatgcc
     1741 ctgcacgatg tacatagagg atgcgaagct ggccgtgctc aatgcggtgc agaggagggc
     1801 cagcacaaag gttgcgcgaa tgcagttctt catagtctct tctccaaaag tatgtggagg
     1861 tggctatcgg cccaatagcc aacattgacg gtgacgagcg ccgctcaatg actgcttgcg
     1921 aagttccccg agagcgtatc gctctcgatc agctgaaacc cggactgcac gccgaagttc
     1981 tgaaggctgc cgtgaaattc atcgcaaaga aatagcgcgg gggtgcgaac gagatcagtc
     2041 cgctgaactc ctgtgcgggc tagccgcgag ctttgaaggg aaaaattgcg tcctgcttgg
     2101 aggcgatggg cgagccgtta tgcgtggcag gatggaagcg ccagttgtcg agcgcggcca
     2161 ggcaacggct gtcaatttcc gggcccaggc tttgcagcac aatcttgcga gtgatttgtc
     2221 cgctctcgtc gatcgtgatc tcgatgacct cgttgccggc ggcctccggc aattgccagg
     2281 gatacacgac cggatcggac gt
//
LOCUS       DASPUL010000302         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_736705, whole genome shotgun sequence.
ACCESSION   DASPUL010000302 DASPUL010000000
VERSION     DASPUL010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736705"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..811)
                     /gene="thpR"
                     /locus_tag="VEJ00_07450"
     CDS             complement(215..811)
                     /gene="thpR"
                     /locus_tag="VEJ00_07450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HXX01031.1"
                     /translation="MRLFIALDIDEAIRERIARFMDGVRGFAENARWVQPESLHLTLK
                     FIGEQPEAAVEQIKQALGTIRASAAEIQFRGYGFFPTPKSARVFWVGMESGPQLATLA
                     KAIDDQTASLGIPKEERAFSPHLTLARGGGGSGSPRWRKGDGPNRTFQQLQEKLSALP
                     APEFGTMTAREFFLYQSQLSPKGSKYTKLARFELTTGG"
     gene            complement(894..>2090)
                     /locus_tag="VEJ00_07455"
     CDS             complement(894..>2090)
                     /locus_tag="VEJ00_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HXX01032.1"
                     /translation="RMDTNSLYLTDQLNRLGVDVIFKSIVGDNLRLLVAAAQHGLFRS
                     DILLFSGGLGPTEDDLTREAVARALGVGLRRDQEILTRLEQRFAERGWKMAANNAKQA
                     DVLEGATVLPNPNGTAPGQWISGQFDGRERTIVLLPGPPHELKSLFESEVCERLRAKI
                     PPASIAIRTLKVAMLGESQVDSRVAPIYKRYSEVETTILAGAGEVELHFKSRAESEDA
                     AQRRVDEVADLVEDELDDAVFSRDGQSLEQIVGYWLQMRNATLAVAESCTGGLLAERI
                     TSVSGSSRYFVGGAVVYSNTLKTELAGVPADMIERHGAVSREVAAALAEGIRYRCEST
                     LGVGITGVAGPTGGTEEKPVGLVFHALASDSGTEVVQRKFPGDRKRVRRFASTMALDM
                     VRRKLM"
BASE COUNT          369 a          635 c          681 g          405 t
ORIGIN      
        1 acaggcgacc tgcgcgagac gttggtagca ccgatgttgc cgctgccgct ctgccgcacg
       61 tcttcgcccc ggaagatgcg gacaagaaga tatccgaagg ggatcgagcc cagcaggtag
      121 cttgcggcgg cgatgaggag aagttttagc aagatccctt ttgcgtcctt cttacgtgcc
      181 tggcggtcgt tgcgagtccc aggtttcctg aaggttaacc gcccgtggtc agttcgaatc
      241 gcgccagctt cgtgtatttc gatcctttcg gcgacaactg gctttggtac aagaagaact
      301 cgcgggcggt catggtacca aactccggag cggggagcgc ggacaacttt tcctgcagct
      361 gctgaaaggt gcggttgggg ccgtctccct tgcgccagcg cggcgagccg gaaccgccgc
      421 cgccgcgagc gagcgtcagg tgcgggctga aggcgcgttc ttccttcgga atgccgagcg
      481 aggcggtttg gtcgtcgatc gccttggcaa gcgttgccag ttggggtccg gattccatcc
      541 ctacccagaa cacgcgggcc gacttcgggg tagggaagaa gccgtagccg cgaaattgga
      601 tttcggctgc actggcgcga atcgttccca aagcctgctt gatctgctca acggctgctt
      661 cgggctgctc gccgatgaat ttcagcgtta ggtgcagcga ctcaggctga acccagcgtg
      721 cattttcggc aaagccgcgg acaccgtcca tgaagcgcgc aatgcgctcg cggatggctt
      781 cgtcgatatc gagggcgatg aagaggcgca tagagaagtc ccgagtcccg agttgcgagt
      841 cccgagctta aaccgaagtg tgctccctgg gactcgggac ttgtgactcg ggactacatc
      901 agcttccttc tcaccatatc caaggccatg gtggaggcga agcggcggac gcgtttgcgg
      961 tcgccgggga attttcgctg gacgacttcg gtgccgctgt cactggccag ggcgtgaaac
     1021 accaggccga cgggcttttc ctctgtgcct ccagtggggc cggccacacc tgtgatgccc
     1081 acgcccagcg tcgactcgca gcggtagcga atgccctccg ccagcgcggc cgccacttcg
     1141 cggctcacgg cgccgtggcg ctcgatcatg tcggccggca cgccggccag ttctgttttc
     1201 agcgtattgg aatacacgac ggcgccgcca acaaaatagc gggaacttcc gctcacggac
     1261 gtaatgcgtt cggccagcag gcctccggtg caggactccg cgacggcgag ggtggcattg
     1321 cgcatctgca gccagtaacc gacgatctgc tcgagcgact ggccgtcgcg ggagaagacg
     1381 gcgtcgtcca gttcgtcctc gaccagatcg gcgacctcgt caacgcggcg ctgggcagcg
     1441 tcttcgctct cggcgcgcga cttgaagtga agttccactt cgccggcgcc agcgagaatg
     1501 gttgtctcaa cttcactgta gcgcttgtag atcggcgcca cgcgcgagtc gacctgggat
     1561 tcgcccagca tggcgacctt cagcgttcga atcgcgatgg aggctggagg aatcttcgcg
     1621 cgcagtcgct cgcacacttc actctcgaac agcgatttca attcatgcgg aggcccgggc
     1681 aggagcacga tcgtgcgctc gcggccgtcg aattgaccgc tgatccactg tccgggcgcg
     1741 gtgccgttcg gattcggcag caccgtcgcg ccttccagaa cgtcggcctg cttggcgttg
     1801 ttggcggcca ttttccagcc gcgttcggca aagcgctgct ccagacgcgt aaggatctcc
     1861 tggtcgcggc gcaatcctac gccaagtgcc ctcgcgaccg cctcgcgggt cagatcatcc
     1921 tcggtggggc caaggccgcc gctgaagagg agaatgtcgg agcggaacag gccgtgttgg
     1981 gcagcagcca cgagcagtcg cagattgtcg ccaacgatgc ttttgaaaat gacatcgacg
     2041 cccaggcggt tgagctggtc ggtgaggtag agcgaattgg tgtccatgcg
//
LOCUS       DASPUL010000303         3854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1832828, whole genome shotgun sequence.
ACCESSION   DASPUL010000303 DASPUL010000000
VERSION     DASPUL010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832828"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VEJ00_07460"
     CDS             <1..638
                     /locus_tag="VEJ00_07460"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXX01033.1"
                     /translation="DLSAFYFDVIKDRLYTYAPNSAGRRSAQTAVWRICEAMARLLAP
                     ILTFTCEEVWQHLPKMPDRTESVHLAAFPEIADVLGSASVPAEDMQQTKDWETLLSVR
                     EQVMKALEEERNSKRIGKSLEAQLFITAADPAYSVLARYRDQLRYLFIVSAVTLVQGS
                     GNGTGGTHIEVKKADGAKCERCWNYSVHVGEDKTYPTICERCSAVLKELEA"
     gene            758..1261
                     /gene="lspA"
                     /locus_tag="VEJ00_07465"
     CDS             758..1261
                     /gene="lspA"
                     /locus_tag="VEJ00_07465"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HXX01034.1"
                     /translation="MSPHRARALHFFLALVVVCVDRVTKRLVASHIAMYTHIQIIPGF
                     FRLTHTENTGAAFSLFADSPAHWKTAFLIGFSLVAMVIVSVLLWKQTRPLSLDGIAFS
                     LILGGAVGNLWDRVASGRVVDFLLFYIRQYQWPVFNLADSSIVVGASLLVIEILFRKV
                     PNGETTI"
     gene            complement(1294..3384)
                     /locus_tag="VEJ00_07470"
     CDS             complement(1294..3384)
                     /locus_tag="VEJ00_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXX01035.1"
                     /translation="MRQAFVASTTLRPMAQQLIQDRSPAAYAGVEAYARAHAKEDAGA
                     LAWLVVGYAHVLDHEFAKAIDPLNRAKPSAGDLGDYVAYYLGTCYLQTGRQGEALATL
                     ADFATTYPDSLLVRDADVSYAGALLSEGRAAEAVALLEKDRAPARSDLELVLGRAYAV
                     EGQNAMAAEAFSNVYYNMPTAADADPAYAELKKLSGVPPPASAMRKTRAELLIKARRY
                     NDAADEYRELLIHAPVEDRPAAQLALADALHRAGRNREAKAELTSLPGANADQAAQRL
                     YILGEVAWAMDENEAFYRTVDQLRQQAPASPWLEVALLSVGNLHLVHHEYDEALAAFR
                     ELQQRFPNGSRASYAHWKAAWLTLRMGRNDEAKKEFDEQIAMYPNGNETSNALYWRAR
                     LAEEDNDFGMARAYYQKLSDRYRNYYYAELGRQRLQKLGAAPNTVERYALLDHVPPLD
                     AEKVVLTEPPADDLHLQKAELLGNGGLVDFAVRELQAAATADGGNWGPAEIARLYLDT
                     EHYDRAIEVMKHSVPSYFAVDIPTLPRQYWEALFPRAYWADLKRFSVANGLDPYLVAS
                     LIRQESEFNPAAVSRANAVGLMQLLPRTGKAVAHQERLKRYSPSQLYTAPVNLELGTL
                     YFRGMVDKFGGSFEYALAAYNAGSDRVEEWLGEGKYRDAQEFVESIPFTETREYVQAI
                     LRNASVYRQLYGAP"
BASE COUNT          701 a         1163 c         1207 g          783 t
ORIGIN      
        1 tcgatctcag cgctttttat ttcgacgtca tcaaggaccg gctttacacg tacgccccga
       61 attctgctgg gcggcgctcg gcgcagacgg cggtctggcg gatctgtgag gcgatggcgc
      121 gtttgctcgc gcccatcctg accttcacgt gcgaggaggt atggcagcac ctgccgaaaa
      181 tgccggatcg tacggagagc gtgcatctcg ctgcttttcc ggaaattgcc gacgtgctgg
      241 gctcggcttc agttccagcc gaagacatgc agcagacgaa agactgggaa accctgcttt
      301 cagtgcgtga gcaggtgatg aaggcactcg aagaagagcg caacagcaag cggatcggca
      361 agagcctgga agcgcagcta ttcatcacgg ccgccgaccc ggcgtattcg gtcctggcgc
      421 gctaccgcga tcaattgcgt taccttttca tcgtgtcggc tgttacgctg gtgcagggct
      481 cgggaaacgg aaccggaggc acgcacattg aagtcaagaa ggccgacgga gccaagtgcg
      541 agcgctgctg gaactattca gtccatgtgg gcgaagacaa aacgtatccc acgatctgcg
      601 agcgttgcag cgcggtgctg aaggaactcg aggcctgagt ccttcttagc ggtctccgcg
      661 gcctttcttc gcgatctttg cggtaaaagc atttgagagc tctcggaggg gctccggtca
      721 caaagctcgt agagatcccg aaaaccaaca actccgaatg tccccccatc gtgcccgcgc
      781 ccttcacttc tttctggcct tggtagtcgt gtgcgtcgac cgcgtgacca agcgtctggt
      841 cgcctcgcac atcgccatgt acacccacat ccagatcatc cccggatttt tccgcctcac
      901 gcacacggaa aacaccggcg ccgccttcag cctgtttgcc gactcgcctg ctcactggaa
      961 aaccgcattc ctgatcggct tctcgctggt cgcgatggtg atcgtctcgg tgctgctgtg
     1021 gaagcagacc cgccccttat ccctggatgg catcgcgttc tcgctgattc tggggggcgc
     1081 agtcggcaat ctctgggatc gcgtagccag cggccgcgtc gtggacttcc tgctgtttta
     1141 catcaggcag tatcagtggc cggtgttcaa tctggccgac agttcgatcg tggtcggagc
     1201 ttccctgctt gtcattgaga ttctgtttcg caaggtaccg aacggcgaaa ccaccatcta
     1261 ggacccaact caaggcaggt tgaagaagtt cctctacggc gctccataaa gttgccggta
     1321 gacgctggcg ttgcgcagaa tcgcctgcac atattcgcgc gtctcagtga acgggatcga
     1381 ctccacaaat tcctgggcat cgcgatactt gccttccccg agccattcct cgacgcggtc
     1441 ggagccggcg ttgtaggccg ccagggcgta ttcgaatgat ccgccgaact tgtcgaccat
     1501 gccgcggaaa tagagcgtcc cgagctccag attcaccggc gcggtgtaca actggctggg
     1561 gctgtagcgc ttgagtcttt cctggtgcgc gacggccttg ccggtcctcg gcaacaactg
     1621 catcaggccg acggcattgg cgcgggacac ggcggccgga ttgaactccg actcctggcg
     1681 gatcagcgag gcgaccagat agggatcgag tccgttggcc accgaaaatc gcttcagatc
     1741 ggcccagtag gcgcgcggga aaagagcctc ccagtactgc cgcggcaggg tcggaatatc
     1801 gaccgcaaaa taactgggga ccgaatgctt catcacctcg atggcgcgat cgtaatgctc
     1861 ggtatcgaga tagaggcgcg caatctcggc gggaccccag ttgccgccat cggcggtggc
     1921 tgcggcctgt aactcccgca cggcaaaatc gaccaggcca ccgttcccga gcagttccgc
     1981 tttctgcaga tgcagatcgt cggccggcgg ctcggtgaga accaccttct ctgcgtcgag
     2041 cggcgggacg tggtcgagta gcgcgtagcg ctctaccgtg ttgggtgcgg cccccagctt
     2101 ttgcagccgt tgccggccca attcggcgta gtagtagttg cggtaccgat cagagagttt
     2161 ctgatagtac gcccgcgcca tcccaaagtc gttgtcttcc tcggccagac gcgcccgcca
     2221 atacaacgcg ttggaggttt cgttgccgtt gggatacatc gcaatctgtt catcgaattc
     2281 cttcttggcc tcgtcgttgc gccccatgcg cagcgtgagc cacgccgcct tccagtgagc
     2341 ataagaggct cgcgatccgt tggggaagcg ctgctgcaac tcgcgaaacg cggccagcgc
     2401 ctcgtcatat tcatggtgga caagatgtag attcccgacg gagagcaagg ccacttcgag
     2461 ccaaggactg gccggagcct gttgccgcag ctggtcgacc gtacggtaga acgcttcgtt
     2521 ctcgtccatc gcccaggcga cttcccccag aatgtagagc ctttgggccg cctgatcggc
     2581 gttggctccg ggcagactcg tgagttctgc tttggcttcc cggttccgtc cggcgcgatg
     2641 cagcgcatcg gccagggcca actgtgcggc gggacgatct tccaccgggg catgaatgag
     2701 cagttcgcga tactcgtcgg ccgcatcgtt gtagcgccgc gctttgatta gcagctcggc
     2761 gcgcgtcttt cgcatcgccg aagccggcgg aggaacgccg gacagtttct tcaactccgc
     2821 gtatgccgga tccgcgtcgg ctgcggtagg catgttgtag tagacgttcg agaacgcttc
     2881 ggccgccatc gcattttgtc cctcaactgc ataagcgcgg cccagaacca gctccagatc
     2941 cgagcgggcc ggggcccggt ccttttcgag cagagcgacg gcttccgctg cgcggccctc
     3001 ggagagcagc gcgcccgcat agctcacgtc cgcatcgcga accagcagcg aatccggata
     3061 cgtggtcgca aagtcggcca gcgtcgccag cgcttcaccc tgccgcccgg tctgcaggta
     3121 gcacgtcccc agatagtagg cgacgtagtc gcccaggtcc cctgccgacg gtttggcgcg
     3181 gttcagggga tcgatggctt tggcaaattc gtgatccagc acgtgcgcat atccgacgac
     3241 aagccaagcc aaagccccgg cgtcctcttt ggcatgggcc cgtgcgtagg cctcgacccc
     3301 ggcgtacgcc gcgggagacc ggtcttggat tagctgttgc gccatcggcc gcaacgtggt
     3361 ggaagcgacg aacgcctgcc gcatgcggcg gatgcgggga gagagcgggc gcttgcgtct
     3421 cttggcactg gtggcgtgag ccgaggccgt cgattttgtg gcagcagctt tcgaacctgg
     3481 ttttcgagtt gtcgatttcg aagttgtgga tttctcggag ggagtggacg cttgaggagg
     3541 tgcaggtttc tcagtagaac tctgcccgcc atcggtctga ggcgtggcag tctggggctc
     3601 ggcgggcgat ggagcggatt ggcctggagg cggagttggg gacgattctt gtgattcgga
     3661 aggggcggag gatcctgccc aggcagaggc agtcagcagt ccgtacgaca gtcccagggc
     3721 gatggcccaa gacagcttgc ggggtgctcg cggcaacggc cgtttttgcg gagatgcgag
     3781 agccagaacc tttttgagag acgccagcac tgctttcaga ctaccccagg gagcaaactg
     3841 tgtctcgact caga
//
LOCUS       DASPUL010000304         4741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_882567, whole genome shotgun sequence.
ACCESSION   DASPUL010000304 DASPUL010000000
VERSION     DASPUL010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882567"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2261)
                     /locus_tag="VEJ00_07475"
     CDS             complement(<1..2261)
                     /locus_tag="VEJ00_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXX01036.1"
                     /translation="MALSSGTRLGPYEIVSPLGAGGMGEVYRARDTRLERTVAIKILP
                     EHLSSSPEARQRFDREARAISALSHPNICHLYDVGQQDTTSYLVMEFLEGETLAERLR
                     KGPLPLEQVLRVGSDICDGLDKAHRSGVVHRDLKPGNIMLTKGGAKLMDFGLAKAPAA
                     PLQSLSGSNSPTLSRPLTTEGTIVGTIQYMAPEQLEGKEADQRSDIFSLGAVLYEMVT
                     GKKAFEGKTTASTIAAILAAEPKPISALQPLSPPALEHLIKTCVAKDPDDRIQTAHDV
                     KVQLQWIAQSGSQTAMAAAKPIVRRTNFLWLVLAGLVLAAAAFLLGTQWSKAPSQSTQ
                     FEVNPPPKTYFNFRGLSGPPVASPDGSKIAFVAFTPGQTATVGLWLRSLESADARFLP
                     GTEGATYSFWSPDGKFLAFFAGGKLKKLDLASGSTIPICDVMEGRGGTWSTDGVILFG
                     VRADAIFRVNASGGKPVRLTTLDEAQHETSHRWPQFLPDQKHFLFVAQAPQIPASRLF
                     AAALDSPKPVRVEDVNSSAMYSNRHLFYVVDNSLLARAFDPEALRFTGEPAVVAEHVQ
                     NDLQFNFASFSVAGSTLIYQTGAVTPGTRVVSYDRSGKQTVISEETDLVQTLVLSPEQ
                     DRLAASFGLSSGQLNDIWVLDLRKNTRTKLTFDQHSFSPAWSPDGKRLAFDRVAGDAD
                     EIIAKDVSGGGAEGALFKFAGPAPGASGESATQKLYPITWTPDGKYLIYRAPGEIAAL
                     ALTGERKSTTLFQT"
     gene            2567..3127
                     /gene="efp"
                     /locus_tag="VEJ00_07480"
     CDS             2567..3127
                     /gene="efp"
                     /locus_tag="VEJ00_07480"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXX01037.1"
                     /translation="MSIPATQLRPGMIIKHNNDLHSVFSVEHRTPGNLRAFIQAKLRN
                     LRTGAMFEHRFRSPDPIEKINVDEVEMEYLYNDGDSYYFMDTSNYEQTHLTKDTLGDA
                     VDYLIPNLQIKVEFFDGKAVGIELPQTVELTVIETEPGLKSATASSVTKPAKTETGLV
                     VQVPPFINEGEKIKVDTSEGAYLSRA"
     gene            3313..3504
                     /locus_tag="VEJ00_07485"
     CDS             3313..3504
                     /locus_tag="VEJ00_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01038.1"
                     /translation="MGSLRTFAEAFGIFAVSIIVLFVLYMVTEKRATGIGVAHWKGYV
                     LMYVGALYFVLGVILTRLK"
     gene            3599..3898
                     /locus_tag="VEJ00_07490"
     CDS             3599..3898
                     /locus_tag="VEJ00_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HXX01039.1"
                     /translation="MTSTEKATEARRYLVRGRVQGVGFRWFVEREAAMLGIAGWVRNN
                     HDGSVEVLAQGTRDQLLGLHSRLREGPRAARVDAVEVSDAQPVNGLSSFRIEGAW"
     gene            3899..4432
                     /locus_tag="VEJ00_07495"
     CDS             3899..4432
                     /locus_tag="VEJ00_07495"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HXX01040.1"
                     /translation="MTLNSDQLKELIREVPDFPKQGILFYDITTLLKDKVGFATLIDK
                     FSEYYISQSVDLVLGMEARGFIFGPALAYRLNAGFVPVRKPGKLPAATVKQEYALEYG
                     LASLEIHKDAIQKGQRVLIVDDLLATGGTAEATANLALSLGAEIAGLGFVVELDFLKG
                     REKLKNYDVMALLHYDK"
     gene            complement(4510..>4741)
                     /locus_tag="VEJ00_07500"
     CDS             complement(4510..>4741)
                     /locus_tag="VEJ00_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01041.1"
                     /translation="DKILAKIVAQDSGRAQLRGGSNLQILRSWYEYDSEAELAHPRRR
                     LNWNMLLGAVIVVGISASFWTGVGMLIARIWK"
BASE COUNT          941 a         1441 c         1431 g          928 t
ORIGIN      
        1 gtctgaaaca aggtcgtgga ttttcgttct cccgtgagcg ccagtgccgc gatttccccg
       61 ggagcgcggt agatcaggta ctttccgtca ggagtccagg taatcgggta caacttctgc
      121 gtggcgcttt ccccgctggc acccggggcg gggccggcga acttgaacag agccccttcc
      181 gccccgcctc cggaaacatc cttggcgatg atttcgtcgg catcgcccgc gacgcggtcg
      241 aacgccagcc tcttgccgtc cggggaccag gccggcgaga aggaatgttg atcgaacgtg
      301 agttttgtac gagtgttctt gcgcaggtcc aggacccata tgtcatttaa ttggccggac
      361 gagaggccga aggaagccgc cagccggtcc tgctccgggg aaagcaccag cgtctgcacc
      421 aggtcggttt cctcgctgat tacggtctgt ttcccgctgc gatcatacga aaccactcgc
      481 gtccccggag tcaccgcgcc agtctgataa atcagagtgg aaccggcgac ggagaacgac
      541 gcgaagttga actgcaggtc attctgcaca tgctctgcca ccaccgccgg ttcgccggta
      601 aagcgcaggg cttcaggatc aaaggcccgc gccaacaggc tgttatccac aacgtagaac
      661 agatggcggt tggagtacat ggcgctggaa ttgacgtctt ccactcgcac gggcttgggc
      721 gagtccagcg cggccgcgaa caggcggctg gcaggaatct gcggggcctg tgcgacaaac
      781 agaaaatgct tctggtcggg aaggaactgg ggccaacgat ggctggtctc gtgctgtgcc
      841 tcgtcgagcg tggtaagccg gacgggtttg cccccggatg cgttcacgcg gaatattgcg
      901 tccgcgcgta cgccgaatag aatgactcca tccgtcgacc acgtgccgcc gcgaccctcc
      961 atcacgtcac agatgggaat cgtgcttccg ctcgcaaggt cgagcttctt cagttttccg
     1021 ccggcgaaga atgccaggaa cttcccgtcc ggcgaccaga acgagtaggt ggctccttcc
     1081 gtgccgggga ggaagcgggc atcggccgat tcgagcgagc gcagccacaa gccgaccgtc
     1141 gccgtttgcc ccggcgtgaa cgcgacgaac gcgattttgc ttccgtcggg cgaagcaacc
     1201 gggggccccg agaggccgcg gaaattaaag taggtcttgg gcggcgggtt tacttcgaat
     1261 tgcgtcgact gcgagggagc tttggaccat tgcgtaccaa gcaggaaagc agcagcagcc
     1321 aaaaccagtc cagccaggac cagccagagg aagtttgtcc gccgcactat gggcttggct
     1381 gccgccatgg ccgtctgcga tccgctctgc gcgatccact gcagttgcac tttgacgtca
     1441 tgagccgtct ggatgcgatc gtcgggatct ttcgccacac aggtcttgat caggtgctcc
     1501 aacgccggcg gcgacagtgg ctgcagcgcc gaaatcggct tcggctccgc cgccagaatc
     1561 gccgcgatcg tcgaagctgt ggtcttgccc tcaaaggctt tctttcccgt caccatctcg
     1621 tacaacaccg cgcccaggga gaaaatgtcg cttcgctgat cggcttcctt cccctccaac
     1681 tgctccggcg ccatgtactg gatcgtcccc acgatggttc cttccgtcgt gagtgggcgc
     1741 gacagggtcg gtgagttgga gcccgacagg ctctgcaacg gggccgccgg cgccttcgcc
     1801 agcccgaaat ccatgagctt ggcgccgccc ttggtcagca tgatgttgcc cggtttcaaa
     1861 tcgcgatgca ccaccccgct gcggtgcgcc ttgtcgagcc cgtcgcagat gtcgcttcct
     1921 acgcgcagca cctgctcgag cggcagcggc cccttgcgca gacgctccgc cagcgtctcc
     1981 ccctccagga actccatcac caggtagctg gttgtgtcct gctggccgac gtcgtagaga
     2041 tggcagatgt tgggatggct caacgcggaa atcgctcgcg cctcgcggtc aaagcgctgc
     2101 cgggcctcgg gactgctgga aagatgttcg ggcagaatct tgatggcgac cgtgcgctcc
     2161 aggcgggtgt cgcgcgcgcg gtatacctcg cccatgccgc ccgccccgag cggactgacg
     2221 atctcgtacg gtccgagcct ggtccccgag ctgagagcca tcagtgcgcg ccattataga
     2281 ccgggaacgg agcattcagc atttagccgg gtgccccatt tcccgcgcgc tctttgcgcg
     2341 agaagtgggg attctcgatt ttccgatctg tgatcaaggt caaaggcccc acttctgccg
     2401 cagaaagcgc ggcagaaatg gggcacccga cctaatcctc gcggtttccc atcccccggt
     2461 gccagcgtgt aaaatagtgt gttcacgatc gtagccgcgg aacttttggc aggcctgcct
     2521 tcttccgccg gctccgcagc gcaatcacag gagaatctcg caagcaatgt cgattcctgc
     2581 cacacaactt cgtcccggca tgatcatcaa gcacaacaat gatctgcaca gcgtgttctc
     2641 ggtcgagcac cgcacgcctg gcaacctgcg cgccttcatc caggccaagc tgcgcaacct
     2701 gcgcaccggc gccatgttcg agcaccgttt ccgctcgccc gatcccatcg agaagatcaa
     2761 cgtggatgaa gtcgagatgg agtacctcta caacgacggt gacagctact acttcatgga
     2821 cacgtcgaac tacgagcaga ctcacctgac caaagacact ctcggcgacg ccgtcgacta
     2881 cctgatcccc aacctgcaga tcaaagtgga attctttgac ggcaaggcgg tcgggattga
     2941 gctgccgcag acggtggaac tgaccgtgat cgaaaccgaa cccggcctga agagcgcgac
     3001 ggcgtcgagc gtgaccaaac ccgcgaagac cgagaccggc ctggtggtgc aggtgccgcc
     3061 gttcatcaac gaaggcgaaa aaatcaaggt cgacacgtcg gaaggcgctt acctgtcgcg
     3121 ggcgtagctg tgtcgccatt gaacgcgcgc gattgtcatc ctgagcgaaa ttggcgttcg
     3181 ctgaaagcga acgccaatgc agtcgaagga tccctagccc gttacaagtc tgccggtggc
     3241 gtagcaaggt attctcttaa cgaccgtggt agaacttcct ggccaagtcc gagggcgtag
     3301 ggagaaaaat agatgggatc ccttcgaaca tttgccgaag cattcgggat ttttgctgta
     3361 tcaatcattg tgttgtttgt tctctacatg gtcacagaga aacgggccac aggcatcggg
     3421 gtggcgcact ggaaagggta cgtgctcatg tacgtaggtg cgctttactt cgtgctaggg
     3481 gtgatcttga cgcgactgaa gtgaactcaa ggcgataggg atccttcgac tccgcctcag
     3541 cttcgcgttt gcgaagctga ggctccgctc aggatgacat tgcctggaac tgcgagccat
     3601 gacctcaacc gaaaaagcga ccgaagcccg ccgctacctc gtccgtggac gcgtacaggg
     3661 cgtgggcttc cgctggttcg tggagcgcga ggcggccatg ctgggcatcg ccggatgggt
     3721 gcgcaacaat cacgatggca gcgtcgaagt gctggcccag ggcacgcgcg accagctttt
     3781 gggattgcat tcgcgcttgc gcgaaggccc gcgggcggct cgcgtggacg ccgtcgaagt
     3841 ctccgacgcc cagcccgtca acggtctcag ttcatttcgc attgaaggag cgtggtaagt
     3901 gacgcttaat tccgatcagc tgaaagagct tatccgcgaa gtccccgatt ttcccaagca
     3961 ggggattctc ttttacgaca tcacgacgtt gcttaaggac aaagtcggat tcgcgacgct
     4021 catcgacaag ttctctgagt actacatctc gcaaagcgtc gacctggtgc tgggcatgga
     4081 ggcccgcgga ttcattttcg gccccgcgct cgcctatcgt ctgaatgccg ggttcgttcc
     4141 cgtgcgcaag cccggcaagc tgccggccgc cacagtgaag caggagtacg cgctcgaata
     4201 cggcctggct tcgctcgaga ttcacaaaga cgcgatccag aaaggccagc gcgtgctgat
     4261 tgtcgacgac ctgctcgcca ccggaggcac cgccgaagcc acagccaatc tcgcgctctc
     4321 gctgggagcc gaaatcgcgg gcctgggttt cgtggtggag ttggactttc tcaaaggacg
     4381 ggagaagctg aagaactacg acgtgatggc cctgctgcac tacgacaagt gaaagaacgt
     4441 tgttgaagga aaccgccaag gctggagcca tggcgtttcc attcaacagc caaaatcaat
     4501 agttgatttt tatttccaga tccttgcgat cagcatcccc accccggtcc agaagctggc
     4561 gctgattccc acaacgatca ccgcacccag gagcatgttc cagttgaggc gtcgtctggg
     4621 gtgagcgagt tcggcttcgc tgtcgtactc ataccaggag cgcaggatct gcaggttgct
     4681 gccgccgcga agttgggcgc ggccgctatc ctgagcgacg atcttggcga gaatcttgtc
     4741 g
//
LOCUS       DASPUL010000305         7316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_737535, whole genome shotgun sequence.
ACCESSION   DASPUL010000305 DASPUL010000000
VERSION     DASPUL010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7316)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7316
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737535"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2145)
                     /locus_tag="VEJ00_07505"
     CDS             complement(<1..2145)
                     /locus_tag="VEJ00_07505"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HXX01042.1"
                     /translation="MLLPFVRELFADVETLPALLRVASHLKEGTGRIRVSGLTPTAKA
                     LLLVLLRRMADRPLVVVVSDNRAVEDFVPILQGFCDLTVAADAESVIALPARDVLPFQ
                     NLSPHPELQEQRATALWKIATGRASIVVTPVAATAIRLRSADYYSDLARTLRRGESFD
                     VDALLAHLNTVGYASADVVEMPGQYAIRGGILDVYSPEAERPVRVEFFGDEVESIRRF
                     DPASQRSSNPMDEALLLPLTETPVSDQLLGAIHTRLSGKRITGSEEIVEAAVRSGGVT
                     TFPGWEFYAPVAGADQTVFDLIPRAAVALDEPDLLRSEFDRFWARVEEAHERSGVGNL
                     VRPPDLYLPPDDWWQKVASLHGADVEHLGIARGEDDNSISFLTQPTPRFHGAVPSMLE
                     EVQKLTREGKQVLFAVPNTGEVERLADIFTEYNVSFRLGSRTRGGESYADETSYFAGE
                     VQTTTLAKAYVPDGVVFPDAKLAIFGARDLFDESDTVASRPQRQKSKVSAFLSDFRDL
                     QVGDYVVHVEHGIGQYQGLKEINQGDGNAEFMLLEYADAARLYVPLTRLDLIQKYRSS
                     EGAKPALSHLGTQAWAKTKARVRKAMEDMADELLKLYAARKMAQGHSFPPGNGFMHEF
                     EDAFEFSETEDQAQAITDVMRDMESGQPMDRLLCGDVGYGKTEVAMRAAFKAVSDNKQ
                     VAVLAPTTVLAFQHYETFKQRFAPFPVTIEMIS"
     gene            2377..2805
                     /locus_tag="VEJ00_07510"
     CDS             2377..2805
                     /locus_tag="VEJ00_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXX01043.1"
                     /translation="MERFGDRWSLLIVRDLMVRGYRTFKHFQQSGEGIATNILAERLR
                     RLEEGGIIIVEREETDGRRLNYRLTEKGIDLAAVLLDLLIWGARHEKADASSSAILRM
                     EKNREEILAEVRRRWRERDPTPILPKFDSERARELARVGK"
     gene            2949..3368
                     /locus_tag="VEJ00_07515"
     CDS             2949..3368
                     /locus_tag="VEJ00_07515"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXX01044.1"
                     /translation="MRVMVLVKANKDSEAGVLPSREGLEEMGKFNMELAKAGILLAAD
                     GLQASSKGKRVKFSGTQRIVTDGPFAETKELLAGYWVWKVQSMDEAVEWLKRAPFDGG
                     TEIEIRQIFEAEDFGDNLSPEMRKYGERWRALSKQSA"
     gene            3444..4049
                     /locus_tag="VEJ00_07520"
     CDS             3444..4049
                     /locus_tag="VEJ00_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX01045.1"
                     /translation="MKKVVLPCLTLLLLAATAFGQTLTQADRDRGLQSLEQTRDGVVA
                     AVKGLSDAQMKFKAAPDRWSVAETLEHITLAEDFLFQNITNNIMKAPAGAADRDTAKI
                     DAFVLSAIPDRSHKAQAPEPLRPTGRWTPAETLDHFVQSRAKTIAFLQSTPDLRAHVV
                     NGPPLNQPLDAYDWLLFISAHSERHTKQILEVKADPNFPKN"
     gene            4106..4567
                     /locus_tag="VEJ00_07525"
     CDS             4106..4567
                     /locus_tag="VEJ00_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01046.1"
                     /translation="MSTKPTHEQAQLHLQVFEMRREARLRQARDWFFKNYFAESFEES
                     MRIAGPGTEAGTNVMMVLGYWEQACALLNYGLLHEDLFFETSGEFFGVWERVKAVVPA
                     CREKFVYKDYLAHLEKTATRYEAWADKRTPGHTAAMREWMKQMRAQMGKAA"
     gene            4642..5184
                     /locus_tag="VEJ00_07530"
     CDS             4642..5184
                     /locus_tag="VEJ00_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXX01047.1"
                     /translation="MRRLIVSNLMSLDGFFESDSRQLDFFVLDEEFFEYAREMLRNAD
                     TLLFGRDTYLHMAAYWPSAPRDEIADQMNNVPKIVFSRTLKGAEWKNSRLVSGDAADE
                     ILRLKQQGGKDLVILGSAKLASFLLQKGLIDEYRVLLQPVLIGSGTPMFGGIKERLRL
                     KLTSTRSFASGVTLLGYQKA"
     gene            5242..6420
                     /locus_tag="VEJ00_07535"
     CDS             5242..6420
                     /locus_tag="VEJ00_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01048.1"
                     /translation="MSRVRVLVGTRKGAFVLTSDGKRDTWEVSGPHFAGWEIYHMKGS
                     PADPNRLYVSQTSGWFGQIIQASDDGGKTWYQPGTPPGEPLPQWPAKASNRFVYDTSP
                     ETGKPLTTHQFYDGTQHPWEFKRVWHLEPSLSDPDTVYAGVEDAAIFRSGDGGQSWQE
                     LAGLRGHGTGPKWQPGAGGMCLHTIILDPSNPQRMYIAISAAGAFRTDDGGKSWKPIN
                     KGLYSQYIPNPTAEVGHCVHHIAMNRHRPNVLFMQKHWHVMRSDDAGEQWNKVSGNLP
                     TDFGFVIDVHAHEPETIYVVPIKSDGEHYVMDGKLRVYRSRTGGNEWEPLTKGLPQKD
                     CYVNVLRDAMAVDSLDKCGIYFGTTGGQVYGSADAGDSWTPIVRDLPAVLSVEVQTLA
                     "
     gene            6436..6894
                     /locus_tag="VEJ00_07540"
     CDS             6436..6894
                     /locus_tag="VEJ00_07540"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXX01049.1"
                     /translation="MPKITTFLWFDHQAEEAANHYISIFKSGKILSVHRVEGKVLALT
                     FELEGQQFMALNGGPLFKFTEAISLFVDCDEQQEVDELWEKLSAGGEPGRCGWLKDKY
                     GLSWQIIPKALMRLMSDPNPAKSQAVLQAMMKMNKIVIAELQNAYDGKAA"
     gene            6987..>7316
                     /locus_tag="VEJ00_07545"
     CDS             6987..>7316
                     /locus_tag="VEJ00_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01050.1"
                     /translation="MARVLRKQGEFEELIECLWSDDPIVCMRAADAAEKISVRKPALL
                     AAFKAELLGLADETTQAELRWHLALMLPRLRLTSRERARATLRLQDYLNDRSSIVKTC
                     ALQGLAEL"
BASE COUNT         1532 a         1918 c         2506 g         1360 t
ORIGIN      
        1 gctgatcatc tcgatcgtga ccgggaacgg ggcgaagcgt tgcttgaatg tttcgtagtg
       61 ctggaaggcg aggacagttg tcggcgcgag cacggcgacc tgcttgttgt cgctgaccgc
      121 cttgaatgcc gcgcgcatcg cgacttctgt cttaccgtag ccgacgtcgc cgcagagcaa
      181 gcgatccatg ggctgaccgg attccatgtc gcgcatgacg tcggtgatgg cctgcgcctg
      241 gtcttcggtc tcgctgaatt cgaaggcgtc ttcgaactcg tgcatgaatc cgttgccggg
      301 cgggaacgag tggccttgcg ccatcttgcg ggcggcatac agcttgagca gctcgtcggc
      361 catgtcttcc atggctttgc gcacacgggc cttggtcttg gcccaggctt gcgtgccgag
      421 atgactgagg gcgggcttgg cgccttcgga ggagcggtat ttctggatca ggtcgagccg
      481 ggtgagcggg acgtagaggc gcgcggcatc ggcgtattcg agcagcatga actcggcgtt
      541 gccgtcgccc tgattgattt ctttcaatcc ctggtattgg ccgatgccgt gttcgacgtg
      601 gacgacgtag tctccgacct gcaggtcgcg aaagtcggag agaaacgccg agaccttaga
      661 tttctggcgc tgcgggcggg aggcgaccgt gtcagattcg tcgaacaggt cgcgggcccc
      721 gaagatggcg agtttggcgt cgggaaacac tactccatcg gggacgtaag ctttggcgag
      781 cgttgtggtc tgaacttcgc cggcgaagta gctggtttcg tcggcgtagc tctcgccgcc
      841 gcgtgtacgg cttccgaggc ggaagctgac gttgtattcg gtgaagatgt cggccaggcg
      901 ctcgacttca ccagtattgg gcacggcgaa gagcacctgc ttgccttcgc gggtgagttt
      961 ctggacttcc tccagcatcg acgggacggc gccgtgaaag cggggagtgg gctgagtcag
     1021 aaacgagatc gagttgtcgt cttcgccgcg ggcgatgccg aggtgctcga cgtcggctcc
     1081 atgcagcgac gcgacttttt gccaccagtc gtcggggggc aggtagagat cgggggggcg
     1141 gacgagattg ccgacgccgc tgcgttcgtg ggcttcttcc acgcgagccc agaagcggtc
     1201 gaattcggag cgcagcagat cgggttcatc gagcgccaca gcggcgcgag gtatgagatc
     1261 gaaaacggtt tggtcggcgc cggcgacggg ggcgtagaac tcccagccgg ggaaggtagt
     1321 gactccgccg gagcgaacgg cggcttcgac aatttcttcc gagccggtga tgcgcttgcc
     1381 ggaaaggcga gtgtgaatcg cgcccagcag ttgatcgctc acgggagttt cggtgagcgg
     1441 aaggagcagg gcttcgtcca tggggttgga tgagcgctgc gaggcgggat cgaagcgacg
     1501 gatggattcg acttcgtcgc cgaagaattc aacgcggacg gggcgttcgg cttcgggcga
     1561 gtagacgtcg aggatgccgc cgcggatcgc gtactggccg ggcatttcga cgacgtcggc
     1621 cgaggcgtag ccgacagtgt tgagatgagc taggagggca tcgacgtcga aggattcgcc
     1681 gcgtcgcaga gtgcgggcga gatcggaata atagtcggcg gagcgcaggc ggatggcggt
     1741 ggcggcgacc ggagtgacca caatcgacgc tcttcccgtg gcaattttcc acagagcggt
     1801 ggcgcgctgt tcctgaagtt cgggatgggg agagagattc tggaacggga ggacgtcgcg
     1861 cgcggggagc gcgatgacgg attcggcgtc agcggcgacc gtcaaatcgc agaaaccttg
     1921 cagaatgggg acgaaatcct ccaccgcacg attgtcgctg acaacgacca ccaggggacg
     1981 gtcggccatg cggcgcaaga gcacgagcag caaggctttg gccgtgggcg tgaggccgga
     2041 gacacgaatc cgccccgtgc cctccttgag gtgggaggct acacgcagca aagcgggaag
     2101 cgtttccacg tccgcgaaga gctcgcggac gaagggaaga agcatgcaag ttgattctaa
     2161 caaggaacgg ggagcggcgt tggggtcggt tcgtcgcgtt ggcggcggcc tcctgcaggg
     2221 acgggcgcct cgctttgctc ggcgggacgg ccgaggcggc tgtccctaca ctgctcgtcg
     2281 ttgacttgca atatgcaagt aatagttgtt agggtatgct gcgtggctgg gaagggacaa
     2341 tcaagacgcc gttccgcttg tccaataaac ttatccttgg agcggtttgg ggaccggtgg
     2401 tcgctgctga tcgtgcgcga tctgatggtg cgcgggtacc ggactttcaa acactttcag
     2461 caatcaggag aagggatcgc caccaacatc ctcgccgagc gcttgcgccg gctggaagaa
     2521 ggcgggatca tcatcgtaga gcgagaggag accgacgggc gcagactgaa ctatcgtctg
     2581 acggagaaag ggattgacct ggccgcggtg ctgttggatc tgctgatttg gggcgcgcgg
     2641 cacgaaaagg cggacgcttc gagctcggcg atcctgcgga tggagaagaa ccgcgaggaa
     2701 atcctggcgg aagtgcggcg gcgctggcgg gaacgagacc cgactccgat acttcccaaa
     2761 ttcgacagcg agagggctcg ggagctggcg cgagtcggca agtaggtatg cgcgctgggg
     2821 ttggcctttg aaaaagaaag tgccgttttg tcgattttag ccgcgtcact cgtcttacca
     2881 gtgaggcgcc agtttgcaag tagcggactg ggccgagaat caactcataa cgtacacgga
     2941 ggaatgacat gcgagtgatg gtgctggtga aggcaaacaa ggattctgag gcgggggttc
     3001 ttccctcgcg cgaggggctc gaggagatgg gcaagttcaa catggaactg gccaaagccg
     3061 gcatcctgct ggcggctgac ggcctgcagg caagctcaaa gggcaaacgc gtgaagttct
     3121 caggaacgca gcgcattgtg accgacgggc cgttcgcgga aaccaaggaa ctgctggcag
     3181 gatactgggt gtggaaggtg cagtcgatgg acgaggctgt ggaatggctg aagcgcgcgc
     3241 cattcgacgg aggaacggag atcgagatcc gtcagatatt tgaggcggag gatttcggcg
     3301 acaatctgtc gccggagatg aggaaatacg gagagcgttg gcgtgcgcta tccaagcaaa
     3361 gcgcctagaa ctggaactgg ccccgaaaca aaattgaagc tttcggcggt gatcgacgtt
     3421 caaaagtgac tggaggattt gacatgaaga aagtcgttct gccttgtctc accctgctgc
     3481 ttctcgccgc taccgcgttc ggccagaccc tgacgcaagc cgaccgggac cgcggcctgc
     3541 aaagtctgga acaaacccgg gacggagtgg ttgcggccgt gaagggactg tcggatgcac
     3601 aaatgaagtt caaggcggcg cccgatcgat ggtcggtcgc tgaaacgctc gagcatatta
     3661 ccctcgcaga ggacttcctc tttcaaaaca tcaccaacaa catcatgaaa gcgccggcgg
     3721 gagccgcaga tcgcgacacg gccaagatcg acgcgttcgt actctctgcg attccggacc
     3781 gcagccacaa ggcgcaggcg ccggagccgc tgaggccgac cgggcgctgg actccggcgg
     3841 aaaccctgga tcatttcgtg cagagccggg ccaagacaat cgcattcctg caatcgacgc
     3901 cggacctgcg ggcgcacgtg gtgaacggcc cgccgttgaa ccagccgctg gacgcctacg
     3961 actggctgtt gtttatcagc gcgcacagcg agcggcacac caagcagatt ctcgaagtga
     4021 aggcagaccc gaatttcccc aagaactgat ggggactcaa ccggccactt cgtcctgaaa
     4081 aaataaacac ataaggagaa ggaagatgtc aacgaaacct acgcatgaac aggcgcaact
     4141 tcatctgcaa gttttcgaaa tgcggcgcga ggcgcggctc cggcaggcgc gagactggtt
     4201 cttcaagaat tacttcgcgg agagcttcga ggaatcgatg cgcatcgccg gacccggaac
     4261 cgaagccggc accaatgtca tgatggtgct gggctactgg gagcaggctt gcgcattgct
     4321 gaactacggg ctgctccacg aagatctgtt tttcgaaacc agcggagaat tcttcggcgt
     4381 ctgggagcgg gtcaaggcgg tcgttccggc ctgccgggag aagttcgtct acaaagacta
     4441 cctggcgcat ctggagaaaa cagccacgcg ctatgaggcc tgggccgaca agcgcacgcc
     4501 tggccatacc gcagccatgc gggagtggat gaaacagatg cgggcgcaaa tggggaaagc
     4561 tgcctgacag gaaacggatc gcgctgacgg gcctcgctcc tgcaacggag gagcggggct
     4621 tcggcgcggc cgaagattct catgcgcaga ctcatcgtct ccaacctgat gtcgctggac
     4681 ggctttttcg aaagcgacag ccgacaattg gacttcttcg tgcttgacga agagttcttc
     4741 gaatatgccc gggagatgct gcgcaacgcg gatacgcttc tgttcgggcg cgacacttat
     4801 ctgcacatgg cggcgtactg gccgtcggcg ccacgcgacg agattgcgga tcagatgaac
     4861 aatgtgccga aaattgtgtt ctccaggact ttgaagggcg cggaatggaa gaactcgcgg
     4921 ctggtgagcg gcgacgcggc cgacgagatc ctgcgtctga aacagcaggg cgggaaagat
     4981 ctggtcattc tggggagcgc gaaactggct tcgtttctcc tgcagaaagg attgatcgat
     5041 gagtatcgcg tgctgctgca gccagtcttg atcgggagcg gcacaccgat gttcggaggt
     5101 atcaaagaaa gacttcgcct caagctgacg agcacgcggt cgtttgcctc cggagtcacg
     5161 ctgctgggtt atcagaaagc ctgagcggag tggttccgcg cgaaattgcg atgaggcgcc
     5221 tggcgccgga aaggatgctc catgagcaga gttagggttc tggtgggcac gcgaaaaggc
     5281 gcgttcgtcc ttacatccga tggcaagcgt gacacgtggg aagttagcgg tccgcacttt
     5341 gccggatggg agatctatca catgaagggg tcgccggcgg atcccaatcg tctgtatgtg
     5401 tcgcagacga gcggatggtt cgggcagatc atccaggcat cggacgacgg cggcaagacc
     5461 tggtaccaac cgggaacgcc acccggcgag ccacttccgc agtggcccgc aaaggcgagc
     5521 aacaggtttg tatacgatac ctcgcctgag accggcaagc cgctcaccac ccaccagttc
     5581 tatgatggca cgcaacatcc gtgggagttc aagcgggtgt ggcatctgga gccgtcgttg
     5641 agcgatcccg acacggtcta tgcaggggtg gaggatgccg cgatctttcg ctcgggagat
     5701 ggcggccaga gctggcagga actggctggc ttgcgcgggc atggcactgg gcccaaatgg
     5761 caaccgggag cgggcggcat gtgcctgcat acgatcattc tcgatcccag caatccgcaa
     5821 cgcatgtaca tcgcaatttc ggcggcggga gcgttccgca ccgacgacgg gggaaagagc
     5881 tggaagccga tcaacaaagg actgtattcg cagtacattc cgaacccgac cgccgaagtt
     5941 gggcactgcg tgcaccacat cgccatgaac cgccaccgtc ccaacgtgct gttcatgcag
     6001 aagcactggc acgtcatgcg ctccgacgat gcgggggagc agtggaataa agtcagcgga
     6061 aatcttccta ccgacttcgg attcgtcatt gatgtgcacg cgcacgagcc ggagacgatc
     6121 tacgttgttc cgatcaagag cgacggggag cactacgtga tggacggcaa gttgcgcgtt
     6181 taccgcagcc ggaccggggg gaacgagtgg gagccgctca ccaagggtct gccgcagaag
     6241 gattgctatg tgaacgtgct gcgcgacgcc atggccgttg attcgctgga caagtgcggc
     6301 atctatttcg gcaccaccgg cggacaggtg tatgggtcgg cggacgcggg ggactcatgg
     6361 actccgattg tgcgcgattt gccggcggtt ttatcggtcg aggtgcagac actggcgtga
     6421 agtgaggaga acgctatgcc gaagattacg acgtttctgt ggttcgacca tcaggccgag
     6481 gaagccgcga accactacat ttcgattttc aagagcggga agattctgag cgttcatcgt
     6541 gtcgagggaa aggtgttggc tttgacgttt gaactcgagg gtcaacagtt catggcattg
     6601 aacggaggtc cgctgttcaa gttcacagag gcaatttcgc tgttcgtcga ctgcgacgag
     6661 cagcaggaag ttgatgaact atgggagaag ctctcggccg ggggagaacc cggccggtgc
     6721 gggtggctca aggacaaata tggtctgtcg tggcagatca ttccgaaggc attgatgaga
     6781 ctgatgagcg accccaaccc ggcgaagtcg caggccgtcc tgcaggcgat gatgaaaatg
     6841 aacaagatcg tcatcgcaga gttgcagaac gcgtacgacg ggaaggcagc gtaagcgagc
     6901 gggatcgcac ctgcatgagc ggcgctggct taagtcggat gttgcaggga ggggaccggc
     6961 gatcgattgg gaaatcgaat caggtcgtgg cgagggttct gcgaaagcag ggcgagtttg
     7021 aggagttgat cgagtgcctg tggagcgatg accctatcgt ctgcatgcgg gcggccgacg
     7081 cggcggagaa aatctctgtg cggaaaccag cgctgctggc ggcattcaag gcggaactgc
     7141 tcggactggc tgacgaaacg acgcaagcgg agttgcgctg gcatctggcg ttgatgttgc
     7201 cacggctgcg gctcacttcg cgggaacgag cgcgcgctac ccttcggttg caggattatc
     7261 tcaatgatcg cagttccatt gtgaaaacgt gtgcgctgca gggtctggca gaactg
//
LOCUS       DASPUL010000306         3903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_326477, whole genome shotgun sequence.
ACCESSION   DASPUL010000306 DASPUL010000000
VERSION     DASPUL010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326477"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="VEJ00_07550"
     CDS             complement(<1..230)
                     /locus_tag="VEJ00_07550"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01051.1"
                     /translation="MAFDVDRVREIADRVAASSGLEVVEVEFLGGGKARMLRVFLDKP
                     AAGASALAGVTHEDCANFSREFGTILDVEDAVP"
     gene            562..2160
                     /locus_tag="VEJ00_07555"
     CDS             562..2160
                     /locus_tag="VEJ00_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HXX01052.1"
                     /translation="MENGTTAGGARPNFFRSALEKHEFVYTAEMVLGRDHNAAEAEQF
                     VREASQSKNGIKVISLTDLPGGNPALPPEAFASYVHEHNLTPIVHLSGKDGNRSFLEA
                     RLHALARAGAENILALTGDAPRDGLQGKAKPVHDLDSVLILRLLRTLQDSVEHDSGNK
                     ASNPAFEFFAGAVVNPYKVREPDLLMQLYKLQLKIAVGAQFIIPQIGYNLRKLYELKQ
                     FMARENIGHVPALANVYVPTAKIARMMQSGELAGCVISDEFIKRLETEKKPQRLERAA
                     LMVAAARDLGFAGAHIGGFGLSHRDFMTIVERAAAIGPDWRGRMDELVFPYPNEFYVL
                     PLGEDGFSDATADYQTCRVKPRASFVQRISETIYRHVIKDGSLGARVFGSRMGSRFKN
                     QAATNSWRQSVWCKLLAPSSLYRKATLGCISCGDCLQDHLNYAGCTMRLCFKELRNGP
                     CGGSRVDGTCEARPDRPCIWNRIYAAALAVGDDLSKFAHVLVPPRDWSLDRTNALVNR
                     FAGLDNAHKRIDLTTVTNKDHRPC"
     gene            2154..2978
                     /locus_tag="VEJ00_07560"
     CDS             2154..2978
                     /locus_tag="VEJ00_07560"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXX01053.1"
                     /translation="MLIIGELINCTRKKAGEAAQRRDAEFFRDLARKQASAGAHMLDV
                     NGDLPDQEGEVLSWLADLVQGTVDLPLCLDSADAKALTTVLPLCKRRPMINSLSDEPT
                     RWTMLPILKEHRPKVIALCTSESGVPTEVDDRLATASRLIDRLTAEGFALDDIYVDPC
                     VLPVAAGPHGPSLLEAVTKIAEAYPGVHISAGVSNVSFGLPLRKLLNESFLLLLMSRG
                     LDTAIVDPCDQQLMMNIMVAEALLGKDERCRRYLRAYREGKLVPTDSSREVAPKVP"
     gene            3097..>3903
                     /locus_tag="VEJ00_07565"
     CDS             3097..>3903
                     /locus_tag="VEJ00_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HXX01054.1"
                     /translation="MIHFPVPEALTFDDVLLLPARSDVIPAQASTQTHLTRTITLNIP
                     IVSAAMDTVTESHMAIALAQQGGLGIIHRNLSIEQQANEVDKVKRSESGMIVDPVTMH
                     PTDKVSDALEVMRKYKISGVPITENGKLVGILTNRDLRFETRFDIPIDNVMTKKNLIT
                     VPVGTTLEEAEKILHQHRVEKLLVVDDKYNLKGLITVKDIQKKLKYPNAAKDSHGRLR
                     VGAAIGATGDFLERAQELAEAKVDVLAVDSAHGHSTKVLDAVKQVKSKLPH"
BASE COUNT          857 a         1192 c         1091 g          763 t
ORIGIN      
        1 ggcaccgcat cttcgacgtc gagaatcgtg ccaaactcgc ggctgaagtt ggcgcagtct
       61 tcatgcgtca ctccggccag cgcgctcgcg cctgcagcgg gcttatcgag aaacacgcgc
      121 aacattctcg ccttgccccc gcccaggaac tcgacctcaa ccacctcgag cccgctcgag
      181 gccgccacgc ggtccgcgat ttcgcgcaca cgatcaacgt caaacgccat gaatccagta
      241 gcttacaggc gatggctgtc acaaccgcag gcccaaacta aaatgtgggc ttgcgcccac
      301 tggtgtgctt cgccaaatgc cggtacacag cagttctcag taccctaata tacgcccgtg
      361 gaggggaaaa agacaaaccc gctgcaaagc caaaggtcgg acatcccagc accaacttgc
      421 ttgccccgac ctcctgcggt cctccgcact acccacctgt gattcccatc actgtccccg
      481 cgtacttcgc tctgttggaa tggattgagt ttgtgaagac tgcgagtccg ccaagtcaac
      541 cggcaggcgt ttcaggaagc catggaaaac ggcacaacag ccggcggtgc caggccgaac
      601 ttttttcgat cggcgcttga aaaacacgaa ttcgtgtata cggcggaaat ggtactcggt
      661 cgcgatcaca atgcggccga ggcggaacag tttgtaagag aagcatccca gagtaagaac
      721 gggatcaagg tcattagcct tacggatctt cccggcggaa atcctgcctt gccccccgaa
      781 gccttcgcct catatgttca cgagcacaac ctcactccga ttgtgcatct cagtggcaag
      841 gacggaaacc gctcttttct ggaagcacgg cttcatgcct tggcacgggc cggcgcggag
      901 aacattctgg ccctgaccgg cgacgcgcca agggatggac ttcagggtaa ggcaaaaccg
      961 gttcacgatc tcgattctgt gctgattctg cgcctgcttc gcacgctgca ggacagcgtt
     1021 gaacacgact ccggaaacaa ggcctcaaac cctgccttcg agttcttcgc gggtgccgta
     1081 gtgaatcctt acaaggtgcg cgagcccgat ctcttgatgc agctctacaa gttacagctg
     1141 aagatcgccg tgggggcgca gttcatcatc cctcagatcg ggtacaacct gcgcaagctc
     1201 tacgagctaa agcagttcat ggccagagag aacattgggc acgtgccggc gctcgcgaat
     1261 gtctatgtac ccaccgccaa gatcgcacgc atgatgcagt caggagaatt ggccggttgc
     1321 gtcatctcgg atgagttcat caagcgcctg gaaacggaaa agaaaccgca aagactggag
     1381 cgtgccgcgc tgatggtcgc agcggctcgc gatctcggct ttgccggcgc ccacatcggc
     1441 ggatttggtc tctcgcatcg agatttcatg accatcgttg aacgtgcagc ggcgatcggc
     1501 ccagactggc gcgggcgaat ggatgaactg gtcttcccct atcctaacga attctatgtc
     1561 ttgccgttgg gtgaagacgg tttcagcgac gccaccgccg actatcagac ttgccgcgtt
     1621 aagccgcgcg cgtcgttcgt gcaacgcatc tccgagacga tttaccgcca cgtcatcaaa
     1681 gacggctctc tcggagcgcg cgtcttcggt tcgcgcatgg gatcacgatt caagaaccag
     1741 gcagcaacaa attcctggcg gcagagtgtt tggtgcaagc tgctggcgcc ttcgagcctc
     1801 taccggaagg caaccttagg ctgcatcagc tgtggcgatt gtcttcaaga ccatctcaac
     1861 tatgcgggct gcaccatgcg cttgtgcttt aaggaattgc gcaacggccc gtgtggaggc
     1921 tccagagtgg acgggacttg cgaggctcgt ccggatcggc cctgcatctg gaatcggatc
     1981 tacgctgcgg cgttggccgt gggcgatgac ctcagcaagt ttgcgcacgt tctggttcca
     2041 ccccgcgact ggagcttgga ccgcacgaat gcgctggtca atcgctttgc cggtctggat
     2101 aacgcccaca agcggattga tctcacgacg gtcacgaaca aggaccacag accatgttga
     2161 ttatcggcga gcttatcaac tgcactcgca agaaagccgg ggaagcggcg caacgaagag
     2221 acgcggaatt cttccgcgac ctcgcccgca aacaggccag cgccggcgca cacatgctgg
     2281 acgtgaatgg agatctcccg gaccaggaag gggaagtttt gagctggctg gcggatttgg
     2341 tccagggaac cgtcgacctc cccctgtgcc tcgacagcgc tgacgcgaaa gcgttgacta
     2401 cggtgcttcc gctctgcaaa cggcggccga tgatcaattc gctgagcgac gagccaaccc
     2461 gctggacgat gctcccgata ctcaaggaac atcggcccaa agtgattgcg ctttgcacct
     2521 ccgagtcggg agtgcccact gaagtagacg atcgcctggc gaccgccagc cgcttgatcg
     2581 accgccttac ggccgagggg ttcgcattgg acgatatcta cgtggatcct tgcgtgctgc
     2641 cggtcgctgc cggtccacac ggtccaagcc tgctggaggc cgtaacgaag attgccgaag
     2701 catatcccgg cgttcacatt agcgccggcg tgagtaatgt ttcattcggt cttccgctgc
     2761 gcaaactgtt gaacgagtcg tttctacttc tgctcatgtc acgcggcctc gatacagcga
     2821 tcgtcgatcc gtgcgatcag cagttaatga tgaacatcat ggtagcggaa gcgctgctcg
     2881 gtaaggacga gcgctgccga cgctacttgc gggcttatcg cgagggtaaa ctcgttccaa
     2941 cggatagctc aagggaagtc gctcccaaag tgccttagtg cgcttgtctc cctcctctgc
     3001 aaactgaggc taccctgcag caggccattt gcctgatctt ccgccgctct ctacaatgaa
     3061 gcatcacccg ctcggctggg cgaatcgcgc aaaactatga ttcattttcc cgttcccgaa
     3121 gctcttacct tcgatgacgt acttctcctg cccgcgcgct ctgatgtgat cccggcgcag
     3181 gcgagcacgc agacgcacct cacgcgcacc atcacgctga atattccgat cgtgagcgcg
     3241 gccatggata ccgtcaccga atcgcacatg gccatcgcgc tggcgcagca gggcgggctg
     3301 ggcatcattc accgcaacct gtcgatcgag cagcaggcca acgaagtcga caaggtgaaa
     3361 cgctcggaaa gcggcatgat cgttgacccc gtcaccatgc atcccaccga caaggtctcg
     3421 gatgccctcg aagtcatgcg caaatacaag atttccggcg ttcccattac tgaaaacgga
     3481 aagcttgtcg gcatcttgac caaccgcgat ctgcgcttcg aaacccgctt cgacattccc
     3541 atcgacaacg tgatgaccaa gaagaacctc atcaccgtac cggtcggcac cacgctggag
     3601 gaggcggaaa agattcttca tcagcaccgc gtagagaaac tcctggtcgt cgacgacaaa
     3661 tacaatctca agggtctgat cacggttaag gatatacaga agaagctgaa ataccccaac
     3721 gccgccaagg attctcacgg ccgcctccgt gtaggcgcgg ccataggggc gaccggcgat
     3781 tttctcgagc gcgcccagga actggcggaa gccaaggtcg acgtgctggc cgtcgacagc
     3841 gcgcacggcc attcgacgaa agttttggac gccgtgaagc aggtaaagtc gaagctgccg
     3901 cac
//
LOCUS       DASPUL010000307         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_326513, whole genome shotgun sequence.
ACCESSION   DASPUL010000307 DASPUL010000000
VERSION     DASPUL010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326513"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1327
                     /locus_tag="VEJ00_07570"
     CDS             146..1327
                     /locus_tag="VEJ00_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXX01055.1"
                     /translation="MSAIKFRGVDFIQFDSLLSDEERLVRDTTRQFIEDNLIPIIEEC
                     NRAGRFPRELVKPMADLGFFGASLKGYGCAGMGNVEYGLMTQELERGDSGVRSFVSVQ
                     SGLVMYPIYEFGSDEQKQKWLPAMQKGEKLGCFGLTEPDFGSNPGGMRMRARKVGSEY
                     VLNGEKMWITSGSIADVAVIWAKVENENDRIRGFLVETDRPGFKADDIHGKWSLRASV
                     TSGLSLQDVHVPESNLLPKSGGLKSPLMCLNQARYGIAWGAVGAAMSCYDTALQYSLM
                     RKQWRDQPIASHQLVQEKLVWMISEITKAQLLVLQVGRLKDAGKAQPHHISMAKRNNV
                     WMALECARMARDILGANGVADDYPIMRHMMNLESVKTYEGTHDVHALIIGQSVTGVDA
                     F"
     gene            1387..1674
                     /locus_tag="VEJ00_07575"
     CDS             1387..1674
                     /locus_tag="VEJ00_07575"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HXX01056.1"
                     /translation="MHIISRKKLKEAAARHPELQGPLDAWLRIAKKASWRNLVEVQTT
                     FSSAEAAGRWTVFNIKGNHFRLITEINYAGGRIYLRHVLTHADYGRGGWKQ"
     gene            1671..2075
                     /locus_tag="VEJ00_07580"
     CDS             1671..2075
                     /locus_tag="VEJ00_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXX01057.1"
                     /translation="MSASAARAEYATLLSRTVPAVIRSEAENERCIRLLEALDAKAAR
                     MTAGERRLADLLTLLIEDFEERHYALKPADPVTVLRELMESNKLKQKDLIDVFGTASI
                     VSEVLSGKRGLNADHIRKLSRRFQVSPEVFFY"
     gene            complement(2084..2533)
                     /locus_tag="VEJ00_07585"
     CDS             complement(2084..2533)
                     /locus_tag="VEJ00_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXX01058.1"
                     /translation="MSTVEQPAIAEHPTAERSVAERKEPTYVFTEKAVVEKSEIARRF
                     LDTRLRLNANVDKANNFLVKRFYNLDHNTYLEGTLPARTKELMGLVASACLRCDDCIF
                     YHAIQAYRLGVSRVEQEESLNVALVVGGSIVIPHLRRAYELLEELYG"
BASE COUNT          563 a          771 c          808 g          520 t
ORIGIN      
        1 gtttgacccc attcgtctgc cctgctaccg tcatatgttt gcccgcagac gctgggcgcg
       61 tccggcttgc ggcaagcttc gctcgttcga tctgccttgc ccgaccgatt gctggggctc
      121 actttaaaac tcacggagac ttcccatgtc cgccatcaag tttcgcggtg tcgacttcat
      181 ccagttcgat tccctgctct cggacgaaga gcggctcgtc cgcgacacca cccgccagtt
      241 cattgaagac aatctgattc ccatcatcga agaatgcaac cgtgcggggc gctttcctcg
      301 ggaactggtc aagccgatgg ccgacctggg atttttcggc gcgtcgctca agggttacgg
      361 ctgcgccggg atggggaacg tcgaatacgg gctgatgaca caggaactgg agcgcggcga
      421 ctcaggcgtg cgcagcttcg tcagtgtgca gtcggggctg gtgatgtatc cgatctacga
      481 gtttggcagc gacgagcaga agcagaaatg gctgcccgcc atgcagaagg gcgagaagct
      541 cggctgcttc ggcttgaccg aaccggattt cggctcgaac cccggcggca tgcgcatgcg
      601 ggcgcgcaaa gtcgggagcg agtatgtgct caacggcgaa aagatgtgga tcacttcggg
      661 gtcgattgcc gacgtcgccg tcatctgggc gaaggtagaa aacgagaatg atcgcatccg
      721 cggttttttg gttgaaaccg accgtcccgg cttcaaagcc gacgacattc acgggaagtg
      781 gtcgctgcgg gcgtcggtga cttcgggcct ctcgctccaa gacgtgcatg tgccggaatc
      841 gaacctgctg ccaaagagcg gcggcctgaa atctcccctg atgtgtctga accaggcgcg
      901 ctacggcatc gcgtggggcg cggtgggcgc ggcgatgtcg tgctacgaca cggccctgca
      961 atactcgctg atgcgcaaac agtggcgcga tcaacccatc gcttcgcatc agctcgtgca
     1021 ggaaaaactg gtgtggatga tcagcgagat caccaaggcg caattgctgg tgctgcaggt
     1081 tgggcgtttg aaggacgcgg gtaaggcaca accgcaccat atctcaatgg cgaagcgcaa
     1141 caacgtctgg atggcgctgg agtgcgcccg gatggcgcgc gacatcctgg gcgcgaacgg
     1201 cgtggccgat gactatccca tcatgcggca catgatgaac ctggagtcag tgaagaccta
     1261 cgaaggcacc cacgacgtcc atgcgctgat catcgggcaa agcgtgacgg gagttgacgc
     1321 gttctaggct gcttgacaag ttcgcaattt gcgaatacta tcttcgcagg atgcgaattg
     1381 taatgcatgc acatcatcag ccgcaagaag ctgaaggaag ctgccgcccg gcatcctgaa
     1441 ttgcaaggcc ctctggatgc ctggcttcga atagcaaaga aagcatcgtg gaggaatctg
     1501 gtcgaggtcc agacgacctt ctcgagcgcg gaggcagccg gcagatggac agtgttcaac
     1561 atcaaaggca accattttcg gctgatcacc gagatcaact acgccggtgg gcgcatctac
     1621 cttcggcatg tactgacaca cgcggattat ggccgaggag gatggaaaca atgagtgcaa
     1681 gtgcagcccg cgccgaatac gccacgctgc tttcccggac cgtccctgct gtgattcgca
     1741 gcgaagctga aaacgagcgc tgcatccgtc tactggaggc acttgatgcc aaggcagcac
     1801 gcatgaccgc gggagagcgg cgcctggctg acctgcttac tttgctgatc gaggacttcg
     1861 aggagaggca ttacgcgctc aagccggccg atccagtcac ggtactccgc gaattgatgg
     1921 aatcgaacaa actcaagcag aaggatctga tcgacgtgtt cggcacggcc agcattgtct
     1981 ccgaggtgct cagcggcaag cgcgggctca atgctgatca catccgcaag ctcagccggc
     2041 gattccaggt gtcgcccgaa gttttctttt actgatcgcg gctttaaccg tacaactcct
     2101 ccaacaactc gtacgcccgc cgcagatgcg ggatcacaat gctgccgccc acgaccagcg
     2161 ccacgttcag cgactcttcc tgctccacgc ggctcacgcc tagacggtaa gcctgaatgg
     2221 cgtgatagaa gatgcagtcg tcgcagcgca ggcacgccga tgcgaccagt cccatcaact
     2281 ccttcgtcct cgccggcagc gtaccttcaa gataggtgtt gtgatcgaga ttgtagaacc
     2341 gcttcaccag aaagttgttg gccttgtcga cgttggcgtt gaggcgcagc cgcgtgtcca
     2401 ggaagcggcg cgcaatttcg ctcttctcga cgacggcctt ttcggtgaag acgtacgtcg
     2461 gctctttgcg ttcggcaacg gagcgttcgg cagtcgggtg ctctgcgata gctggctgtt
     2521 caacggtact catgcgcaaa tggtagcaga acccggcggc tccgacgcaa cagaatgcgg
     2581 cgacggacga cagagactgc atcctgactc agcatggttg acaggccgcc tcgcgcggca
     2641 tgatgattcc cacagaaccc aa
//
LOCUS       DASPUL010000308         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_883450, whole genome shotgun sequence.
ACCESSION   DASPUL010000308 DASPUL010000000
VERSION     DASPUL010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883450"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1947)
                     /locus_tag="VEJ00_07590"
     CDS             complement(<1..1947)
                     /locus_tag="VEJ00_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HXX01059.1"
                     /translation="MKNRASRDHELGMHRSITRRDFLNGIAVSAGGAMASSWLPTLLS
                     AQATSGSPPGAQDAPGYYPPLLTGMRGSHPGAFETAHEVRDGDFWQAAPKLVDTGETF
                     DLVVVGGGMSGLAAAYFYRKQAGAAARILILDNHDDFGGHAKRNEFRPGGRLLLTNGG
                     TVAIESPFPYSKEARGLLTELGIDPPAFEKKYSGAEPYRGLGFGYFFDKETFGAERLV
                     TGAPGGFRQGGNGSKWADFLAKTPLSAEAQQSIVRIQEDKIDYMEGIPQAEKKARLVR
                     MSYKDFLLKIVKAHADVIPFYQTRTHGLYGIGIDAVPALDCWAIHFPGFQGMGLDRVP
                     SKGLTFTALGEVTPQDEYHFHFPDGNASIARLLVRSLVPGSAPGSTAEDIVTAKMDYS
                     RLDRAESPVRIRLSSTAVAVHHVGDAASAKQVDVVYARDQKTYSVRAKGTVLACWNMV
                     IPYICPDLPAEQKQALHYGVKVPLVYTVVQIRNWKAFQKLGIRGVSSPGMYHSSINLD
                     QAVNIGDYKSPQSPDDPILIHMLRTPCQPGLSAREQQRAGHYDLLATSFETFERNIRD
                     QLARVLGAGGFDPVRDIEAITVNRWPHGYAYEYNPLWDPDWPEGKRPCDIARRRFGRI
                     TIANSDAAAAAYTDQAIDQGYRAVQ"
     gene            complement(1952..>2493)
                     /locus_tag="VEJ00_07595"
     CDS             complement(1952..>2493)
                     /locus_tag="VEJ00_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01060.1"
                     /translation="THVAIRNWAAFHKLGIRQIISPGSYHCFTMLDFPVSLGAYKFPS
                     ASQEPMVLFMLRTPCKPGLPRREQYRMGRYELIGTPFSTFERNIRDQLNRMLASGGFD
                     SARDMAAITVNRWGHGYAYEYESLSDPHWAPEERPCVVGRKKFGRISIANSDAGASAY
                     TNAAIDQAYRAVQEQLKDI"
BASE COUNT          481 a          766 c          746 g          500 t
ORIGIN      
        1 ctgcaccgcc cggtagcctt gatcaatcgc ctgatcggta taggcagctg cggccgcatc
       61 cgaattcgca atcgtgattc gaccaaaccg acggcgggca atgtcgcaag gccgcttgcc
      121 ctcaggccaa tccgggtccc acagcgggtt gtactcgtac gcatagccgt ggggccagcg
      181 attgaccgta atcgcctcga tgtcgcgtac cgggtcaaag ccgcctgcac ccaacacgcg
      241 tgccagctga tcgcggatgt tgcgctcaaa ggtctcgaac gaggtcgcca gcaagtcgta
      301 gtgccctgcg cgttgctgct cgcgagcaga aagccccggc tggcagggag tgcgcagcat
      361 gtggatcaga atcgggtcat caggcgactg cggagacttg tagtcgccaa tgttgaccgc
      421 ctgatccagg ttgatcgacg aatgatacat gccgggactc gacacgccgc ggattcctag
      481 cttctgaaac gccttccagt tgcggatttg cacaaccgtg tacacgaggg gaaccttgac
      541 gccgtagtgc aaggcttgtt tctgctccgc gggaaggtcc ggacagatgt agggaatcac
      601 catgttccaa cacgccagta cggttccctt ggcgcgcacg ctgtaggttt tctgatcgcg
      661 cgcgtaaacc acgtcgacct gctttgcgga agcagcgtcg cccacatgat gaactgcaac
      721 cgccgtgctg ctcagacgaa tgcgcaccgg agactccgcg cggtcgagac gactgtagtc
      781 catcttcgcg gtcacgatgt cttccgccgt gcttcccgga gcggagccgg gaacgagcga
      841 tcgcaccagc agtcgcgcaa tcgaagcgtt gccgtccggg aagtggaagt gatattcgtc
      901 ctggggcgtg acttctccca gtgcggtgaa ggtcaatccc ttggagggca cacgatcgag
      961 acccattcct tgaaaaccgg gaaagtgaat cgcccagcaa tccagcgccg gtacggcgtc
     1021 gattcctatt ccatacagac cgtgcgtacg cgtctgatag aacggaatga cgtcagcatg
     1081 cgctttcacg atcttgagca ggaaatcctt gtagctcatg cgcaccagcc gcgccttctt
     1141 ttcggcttgc gggattccct ccatgtaatc gatcttgtcc tcttggattc ggacaatgct
     1201 ctgctgcgct tcggcggaga gtggcgtctt ggccaggaag tcggcccact tcgagccgtt
     1261 cccgccttgc cggaaacccc cgggagcacc tgtaacgagc cgctcggccc caaacgtctc
     1321 tttgtcgaag aagtagccga acccgagacc acgatatggc tccgcgccag agtacttttt
     1381 ctcaaaggcg ggaggatcga tgccgagttc ggtcagcaga ccccgcgctt ccttgctgta
     1441 gggaaacgga gattcaatcg ccaccgtgcc gccgttggtg agcagcaggc gtccgccggg
     1501 gcggaattca tttcgcttcg cgtgaccccc gaagtcatcg tggttgtcga ggatcagaat
     1561 gcgcgcggca gccccagcct gtttgcggta gaagtacgca gccgccaggc cgctcatgcc
     1621 tccgcccact acgaccaggt cgaacgtctc gccggtatcg accagcttgg gagcggcctg
     1681 ccagaaatca ccgtcgcgca cctcgtgggc cgtttcaaac gcgccgggat ggcttccccg
     1741 cattcccgtg aggagtggtg gatagtagcc gggggcgtcc tgcgcgcccg gcggggagcc
     1801 cgacgtcgcc tgcgcgctca gcagcgtggg aagccacgaa ctggccatcg cccctccggc
     1861 gctgaccgcg attccgttta gaaaatcccg gcgcgtaatg ctccggtgca ttcccagttc
     1921 gtgatctcgc gacgctctgt tcttcatagt gttatatatc cttcaattgc tcctggacag
     1981 cgcgatacgc ctggtcgatc gccgcattcg tgtaggcact ggcgcccgcg tcggaatttg
     2041 caatcgagat tcggccaaac ttctttcggc ccaccacaca gggacgctcc tccggcgccc
     2101 agtgcggatc cgaaagtgac tcgtactcgt aggcgtatcc gtggccccag cggttgacgg
     2161 tgatggctgc catgtcgcgg gcagagtcga atccgccgga tgcgagcatg cggttcaatt
     2221 gatcccggat attccgttcg aaggtggaaa acggcgtgcc gataagctcg tatcttccca
     2281 tccggtactg ctctcgccgc ggcaggccag gcttgcacgg cgtgcgcagc atgaacagca
     2341 ccatcggctc ctgagaagcg cttgggaatt tgtatgcacc caggctcacg gggaaatcca
     2401 gcatggtaaa gcagtgataa ctaccgggag aaattatctg tcggatgccc agcttgtgaa
     2461 acgccgccca gttgcgaatg gctacatgag tgt
//
LOCUS       DASPUL010000309         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1281688, whole genome shotgun sequence.
ACCESSION   DASPUL010000309 DASPUL010000000
VERSION     DASPUL010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281688"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VEJ00_07600"
     CDS             complement(<1..1371)
                     /locus_tag="VEJ00_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HXX01061.1"
                     /translation="MKRLLSFTAGVVLMTLLAAPPVPAWTQTQPAPVKPLTIEAIYQP
                     GGLTGRGPENVAWSPDGTKLSFVQRNARGNEGELWEVNAATGDKKILVNAAKLAALVP
                     DVNNVKNERERERLTRYHVAAYQWAPDSKRMIFDSFGQLWLYDLGSQTAVQFTSSPEA
                     SENPKFSPDGSHVAYVRKHNLYVHSVDGKGERQLTHDTSDNLLNGEIDWVYAEELSVR
                     SNYFWSTDGKEIVFLHMDETNVPTHPLVDLLPTHATVDLQKYPQVGDPNPAVKLGVVD
                     AEKGKVRWVSLSSDEDIYIPRFGWASDGVIWAEVLNRAQDHMDLYFVDAKSGKSRIAL
                     TESTPGAWIDFDHVEVRFLAAGRFLWPSWRDGNMHLYLYSFDAKNPLAADAKLERQLT
                     QGNFEVLGIEGVDDAAGTVFFAANKEDPREVHIYSVQLDGSGFKVLTPEEGEDLADFS
                     DDGKHYL"
     gene            1501..>2544
                     /locus_tag="VEJ00_07605"
     CDS             1501..>2544
                     /locus_tag="VEJ00_07605"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HXX01062.1"
                     /translation="MLPDIENLLRLQEADKEIRRLQDEIAELPKRVAAIEQKLAGTKA
                     QLEKAQTNIKADEAARRKHETAITDLRGKISKYRDQSLEVKTNDQYKALLHEIQFAEK
                     EIAATEDKILELMVNADTRAGEVKAAQAELKAETAEIEKEKEQARLRTAEDEKLLAEW
                     KGKRDQLRSGIDDDLLRHYERVSKFRGTGISEVRDHKCMTCQVMLRPQTYNDVRSGQQ
                     IVYCDSCQRVLYLNPADELVEQKPTIHRPRRHHPKIDAPQAWYYRENYGDAGEVYVVL
                     TNQGGQASRRIYEIHTGRMIGDILIREGDYRQAFPEDITGAIRLNGDWSEEELEAWGT
                     EAPMVVLDALHADL"
BASE COUNT          558 a          812 c          709 g          465 t
ORIGIN      
        1 caggtagtgc ttgccgtcgt cgctgaagtc ggccaggtct tcgccttcct cgggcgtgag
       61 aactttgaac cccgatccat ccagttgcac cgagtagatg tgcacctccc gcggatcttc
      121 cttgttcgcg gcgaaaaaca ccgtcccggc ggcgtcgtcc acgccctcga tgcccagcac
      181 ttcgaagttg ccctgcgtga gttgcctttc cagcttcgca tcggctgcga gagggttctt
      241 cgcgtcaaag ctgtagaggt acaggtgcat gttcccgtcg cgccagctcg gccacaggaa
      301 acgtcccgcg gcgagaaaac gaacctccac atggtcgaag tcgatccacg cgccgggcgt
      361 ggactcggtc aaagcaattc gcgacttgcc cgactttgca tccacgaagt acaagtccat
      421 gtgatcctgc gcgcggttca gcacctcagc ccagatcact ccgtcgcttg cccagccaaa
      481 acgcggaatg tagatatcct cgtcgctgct caacgaaacc cagcgcactt tgcccttttc
      541 cgcatcgacc acccccagct tcaccgccgg gttcgggtcg cccacctgcg gatacttctg
      601 caaatcgacg gtcgcatggg ttggcagcaa gtccaccagg ggatgcgtgg gaacattggt
      661 ctcgtccatg tgcaggaaca caatctcttt gccgtcggtc gaccagaagt agttgctgcg
      721 taccgacagc tcttcggcgt acacccagtc gatctcgcca ttcaggagat tgtcgctggt
      781 atcgtgagtg agttgtcttt ctcccttgcc atcaacggag tggacgtaga gattgtgctt
      841 gcgcacgtag gccacatggc tgccgtcggg tgagaacttg ggattttcgc tcgcctccgg
      901 cgacgaagta aactgcaccg cggtctgcga tccaaggtcg tagagccaga gctgcccgaa
      961 tgaatcgaag atcatgcgct tcgaatccgg cgcccactga tacgccgcca catgatagcg
     1021 ggtcagccgc tccctctctc gctcgttctt cacgttgttg acgtcgggaa caagcgcggc
     1081 cagcttcgcc gcgttaacga ggatcttctt gtcaccggtg gctgcgttta cttcccagag
     1141 ctcgccctcg tttcccctag cgttgcgctg cacaaacgac agtttggtgc catccggact
     1201 ccacgccaca ttctccggac ctcgccctgt cagcccgccc ggttgataaa tcgcttcgat
     1261 ggtcagcggc ttgacaggtg ccggctgcgt ttgtgtccag gctgggacag gcggcgcggc
     1321 cagaagcgtc atgagcacaa caccggcagt gaaggagagc agtcgtttca cgagaagacc
     1381 tcagcagaag aagaagatag tagcggcacg aacgaaggaa gagtgtacac cggtcaggcc
     1441 ccgcccacac ggcctttcgc tgctggctgc atgctagaat caaagcagcg tcaatgccgg
     1501 atgcttcctg acatcgaaaa cctgctgcgc ctgcaagaag cggacaagga aatccgccgc
     1561 ctgcaggacg agatcgcaga acttcctaaa cgcgtcgccg ccatcgaaca gaaactcgcc
     1621 ggaaccaagg cccagctcga aaaagcccag accaacatca aggccgatga agccgcccgg
     1681 cgcaagcacg aaaccgccat caccgacctg cgcggcaaga tctccaaata ccgcgaccag
     1741 tcgctggagg taaagaccaa cgatcagtac aaggcactgc tgcacgagat ccagtttgcg
     1801 gaaaaagaaa tcgccgccac cgaggacaaa atccttgagt tgatggtcaa cgccgacacc
     1861 cgcgccggcg aagtcaaggc cgcgcaggcc gaactgaagg ccgaaaccgc cgagatcgag
     1921 aaagaaaaag aacaggcccg cctgcgcact gccgaagatg agaagcttct ggcggaatgg
     1981 aaaggcaagc gcgaccagct acgctcgggc attgacgacg accttttgcg ccactacgag
     2041 cgcgtttcca agtttcgcgg taccggtatc tctgaagtcc gcgatcacaa gtgcatgacc
     2101 tgccaggtca tgctgcgccc gcaaacttac aatgacgtcc gctccggcca gcagattgtc
     2161 tactgcgatt cctgccagcg tgtgctctat ctgaatcccg ccgacgaact ggtcgagcag
     2221 aaaccgacga ttcatcgccc gcgccgccac catccgaaga tcgatgcgcc acaggcctgg
     2281 tactatcgcg agaattacgg cgatgccggc gaggtttacg tcgtcctgac caatcaaggc
     2341 ggccaggcca gccgccgcat ttacgagatt cacactggcc gcatgatcgg cgacattctc
     2401 atccgcgaag gcgactaccg ccaggccttc cctgaagaca tcaccggagc catccgcctg
     2461 aacggcgact ggagcgagga agagctcgaa gcctggggca cggaagcgcc catggtcgtg
     2521 ctcgacgcgc tgcacgccga tctc
//
LOCUS       DASPUL010000310         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_54269, whole genome shotgun sequence.
ACCESSION   DASPUL010000310 DASPUL010000000
VERSION     DASPUL010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54269"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VEJ00_07610"
     CDS             <1..394
                     /locus_tag="VEJ00_07610"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXX01063.1"
                     /translation="TKRAAAETAMQALSNVHAEARADADNRRTQTRYSCRLGAEVYRT
                     GTSVPNHCCLTDLSSGGCYLEVPLPFPSGSSVEILVRTYEMKLRLRGTVQASHPGYGM
                     GVSFELKTKEERENVKKLTDFVAATTAS"
     gene            complement(426..1379)
                     /locus_tag="VEJ00_07615"
     CDS             complement(426..1379)
                     /locus_tag="VEJ00_07615"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HXX01064.1"
                     /translation="MSQRAVVTGGAGFLGSHLCDALLGQGWSVVAVDNLLTGRRANLE
                     HLRNDSRFEFVEKDVCEPFEVGRVDYVFHFASPASPVDYSTHGIPTLKVGSLGTFNSL
                     DLARKYSAKYFVSSTSECYGDPLEHPQKETYWGHVNPIGPRSVYDEAKRFTEAVTMAY
                     RRYHKVDTRIVRIFNTYGPRMQLNDGRVVPNFMKQALLGEDLTVYGDGQQTRSFCYVS
                     DEIDGFLRLAKSDEHLPVNIGNPDEFPILECAQRVLKVTGSKSRIRHEALPQDDPKQR
                     RPDITKARQLLGWEPKVDLETGLRLSLDYFKKAVAEEVAAK"
     gene            complement(1376..2794)
                     /locus_tag="VEJ00_07620"
     CDS             complement(1376..2794)
                     /locus_tag="VEJ00_07620"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HXX01065.1"
                     /translation="MRIAVVGSGYVGLVAGACFADLGHDVILVDNDQRKLTALRNGQV
                     PIHENFLPELLSRHRGQRLTFSDDLQEAVRQSSVIFVAVGTPPTESGEADLSYVESVA
                     REISGGINGYKVIVEKSTVPVYTSEWVRKIILRNGADPEMFDVASNPEFLREGTAVTD
                     FLFPDRIVVGCDSDRSAKMLREIYAPLTNGSYYERPDAIPQPDRASIPAPIIITSTKS
                     AELIKHASNAFLAMKISFINAVASVCESVGANVNQVVHGIGTDSRIGPRFLNPGIGYG
                     GSCFPKDVMAFRAVARESGYEFRLLDEVMRINEEQRQRFLRKIRSALWTLRGKNLGVL
                     GLAFKGGTDDIRESPALFLVQALLQEGSKITAYDPAAMERTQEVMTSGITYANSPYEA
                     AHNADALLILTEWEEFANLDLNRMRQELRYPIVIDGRNLYDPDVMAAQGFTYYSVGRA
                     TCHPDGLPAGMAKNGLGNGKKA"
BASE COUNT          699 a         1029 c         1035 g          735 t
ORIGIN      
        1 gacgaagcgc gcagcggcag aaaccgctat gcaggctctc tccaacgttc acgcggaagc
       61 gcgcgcggat gccgataatc gcagaaccca gactcgttac tcctgccggc tgggcgcgga
      121 ggtctaccgc accggcactt cagttcccaa tcattgctgc ctgaccgacc tcagttccgg
      181 cggatgctac ctggaagtcc ctttgccatt tccttccggt tcttcggtcg agatcctggt
      241 ccggacctac gagatgaagt tgcgcctgcg cgggacggtc caggcctccc atcccggcta
      301 tgggatgggc gtctcattcg agctgaagac gaaagaagaa cgggaaaacg tgaagaagct
      361 gaccgacttc gtggccgcta cgaccgccag ctaaacgcac cctcgctata tcgcgcgcac
      421 cggatctact tcgccgcgac ctcttcggcg acagccttct tgaagtaatc gagcgacagc
      481 cgcaaacctg tttcgagatc gaccttcggt tcccacccca gcaattggcg cgccttggta
      541 atgtccggcc gtcgctgttt ggggtcgtcc tgcgggagtg cctcgtggcg aattcggctc
      601 tttgatcccg tcaccttcag cacacgctgg gcgcattcca ggatcggaaa ctcatcggga
      661 ttaccgatgt tcaccggcag atgctcgtcc gattttgcca gccgcaggaa tccatcaatc
      721 tcatcgctga cgtagcagaa gctgcgggtc tgctggccgt cgccgtagac ggtcagatcc
      781 tcgccgagca aagcctgctt cataaaattg ggtacgacgc ggccgtcatt gagctgcatg
      841 cgcgggccgt aagtgttgaa gatgcgcaca atccgtgtgt ctaccttgtg gtagcggcgg
      901 taggccatgg tgaccgcttc ggtgaagcgc ttggcctcgt catagaccga gcgtgggccg
      961 atgggattga cgtgtcccca gtaggtttcc ttctgcggat gctcgagggg atccccgtag
     1021 cattcggacg tggatgaaac aaaatatttc gcggagtact tgcgggcaag gtccagcgag
     1081 ttgaacgtcc cgagcgaccc gaccttcagg gtagggatgc catgcgtgct gtagtcgacc
     1141 gggctagccg gactggcgaa gtggaacacg taatcgaccc ggccgacttc gaagggctcg
     1201 cagacgtctt tctccacaaa ttcaaaacgc gagtcattgc ggagatgctc aagattcgcg
     1261 cgccggccgg tgagcaggtt gtcgaccgcc accacactcc agccttggcc gagaagggca
     1321 tcgcacaggt gcgatcccag aaaaccggca cccccggtga ccaccgcgcg ctggctcatg
     1381 ctttcttgcc gtttcccaat ccgttcttcg ccatgccggc gggcaagccg tcaggatggc
     1441 aggtggcgcg ccctacgctg tagtaggtga agccctgggc ggccattaca tccggatcat
     1501 acaggttgcg gccgtcaatg acgatggggt agcgcaattc ctggcgcatc cggttgagat
     1561 cgaggttggc gaactcttcc cattcggtca ggatcagcaa ggcgtcggcg ttgtgggcgg
     1621 cttcatacgg cgagttggcg tacgtaatgc cagaggtcat cacctcctgc gtgcgttcca
     1681 tcgcggccgg atcgtatgcc gtaatcttgc ttccttcctg cagcaaggcc tgcaccagaa
     1741 acagcgcggg tgattcgcga atgtcgtcgg tgccgccctt gaaggcgagt cccagcacgc
     1801 cgaggttctt gccgcgcagt gtccataatg cgctgcgaat cttacgcagg aagcgctggc
     1861 gctgttcttc attgatgcgc atgacttcgt caagcaagcg gaactcataa ccgctttccc
     1921 gcgccacggc gcgaaacgcc atgacatctt tcggaaagca ggagcccccg tagccgattc
     1981 cgggattcag gaagcggggt ccgatgcgcg aatcggtgcc gattccgtgc accacctgat
     2041 ttacgttggc gcctacggac tcacaaaccg aagccacggc gttgatgaag gagatcttca
     2101 tggccaggaa cgcattcgag gcgtgcttga tcaactctgc gctcttcgtg ctggtgatga
     2161 tgatgggcgc ggggatcgac gcgcggtcgg gttgaggaat agcatccggg cgctcgtagt
     2221 agctgccgtt ggtgaggggc gcgtagatct cgcgcaacat cttcgccgat cggtcgctgt
     2281 cacaaccgac cacgatgcgg tcgggaaaca ggaagtcggt gactgcagtg ccttcgcgca
     2341 gaaattcggg atttgaggcc acgtcaaaca tttctggatc ggcgccgtta cgcagaatga
     2401 tcttgcgcac ccactcgctg gtatagacgg gaaccgtgct cttctcgacg atgactttgt
     2461 atccgttgat gccacctgag atttcccggg cgacagactc gacataagac aagtccgctt
     2521 cgccgctctc ggtcggaggc gtgccgacgg cgacgaaaat cacagaactc tgccgcaccg
     2581 cttcctggag atcgtcggag aaagtcagac gctgcccgcg gtgccggctc agcagttccg
     2641 gaaggaaatt ctcgtggatg ggcacctgtc cgtttctgag cgccgtcagt ttccgctggt
     2701 cattgtcaac cagaatgacg tcatgaccga ggtcggcaaa acaggcccct gcgaccagac
     2761 ctacgtagcc cgagcctacg acagcaatcc gcatgtgcga ctcccttgga acggatgttc
     2821 tgaaacagag ttcatgacga acacgattgg gggcggctcg aaggggattt accggagtgc
     2881 tttgggaaca tttactcttt gccccaaatt gttccaaaag tttaacagct tctgaccact
     2941 ttggcgagag acgaattacc ctaaaacctt tctccagtcg cgccaccccg gcgttttttt
     3001 gcccctttcc gactcacagg acaaactagt ggtttgatgg gcttacctcg ttcggaatct
     3061 gccatcagcg tggcaagccg cctctgccgg gtttcctctt ttttcgcact gatgacccag
     3121 aatgtagccg tacggcggta ccagggcggc tgggactgga agaaatccca gagcgcctcg
     3181 tccacggctt cagggtaggt aatactgggc tttgcggacg ttttcttgta agagccgacc
     3241 cataagtcga ggcctgtctc gccgttagct cttggccagt ttctgaaatc cgcggccgat
     3301 gggaagaaga cgaccttcat atgtgcgaga gaaagcgacc cagtttctca aaacgcgaca
     3361 caggatagca ggcagccggt caggcctgcc ttccccttct gaccctgcct ccccgcaggg
     3421 gcccgaaagt cgcattgaca agggggcgtt ttggagtgaa actccgctag ggacgcagcg
     3481 gattcgagtt ttgcacag
//
LOCUS       DASPUL010000311         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_886175, whole genome shotgun sequence.
ACCESSION   DASPUL010000311 DASPUL010000000
VERSION     DASPUL010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886175"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VEJ00_07625"
     CDS             <1..747
                     /locus_tag="VEJ00_07625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXX01066.1"
                     /translation="MHPAIEVRELTKKYEEGTSGTLALRGVDLAVQPGELLMLMGPSG
                     SGKTTLLSIMGCILTATSGSVRVAGREVVGLREKDLPALRLEHIGFVFQGFNLFPTLT
                     AGENVELMLDLKGVSVAKAKTRAEELLEQVGLGEKYGSFPSDLSGGQKQRVAIARALA
                     GDPGIILADEPTAALDSHTGRKVMEMMSDLAHKRGRAVVVVTHDSRVLSFADRIVKIE
                     DGAVATAQESETVPAGLMAHSLPEAALELV"
     gene            794..1888
                     /locus_tag="VEJ00_07630"
     CDS             794..1888
                     /locus_tag="VEJ00_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXX01067.1"
                     /translation="MSQRFSIGSLLKSRTNVVIILGVFLLTASLVLASRRAHESTVEA
                     KAASSSRIPLLIAGPGRVEPVSEDIKIGSELSGRLKSVYVEEGDAIHRGQVLAELENA
                     DYRAQVESSRANVVAKEATLRKVINGARHQERDEAWSSVNEAKAVMENAQSELHRRQE
                     LFSAGVVSREELDRYTSDADVAKAKYESAVQHHALVDDRAREEDRSFAEADLQLAQGQ
                     LAEAEARYEKTFIRSPIDGTVLRKHHRSGESVSNSSTVPDPILTIGDRKTLRVRMDVD
                     ETDVSKVRVGQKAYVTADAFGTQKFWGRVVRVGQQLGPKNVRTDEPTEKVDTKILETL
                     VELDPGSQLPDGLRVDAFIVPDGGELAQNR"
     gene            2041..2439
                     /locus_tag="VEJ00_07635"
     CDS             2041..2439
                     /locus_tag="VEJ00_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01068.1"
                     /translation="MAGIILITVPSIQYGGYFLLTSLMDRTSRYMDNPLRQNFFRAGH
                     AHAGVFVILALLCQILADAANLPTPWLWLARIGVPLAAILVPLGFFLSVPAPSSVQPN
                     GFVAFIYVGAVLLAASVLTLGIGLLRSQAV"
     gene            complement(2812..>3621)
                     /locus_tag="VEJ00_07640"
     CDS             complement(2812..>3621)
                     /locus_tag="VEJ00_07640"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HXX01069.1"
                     /translation="MATSKEKQTEQPKRHLTMKIPPIVSRQDWEAARQQLLVKEKALT
                     RSRDALAAERRRMPWMAVEKQYEFVGPKGKASLLDLFEGRPQLIVYRAFFEPGVFGWP
                     DHACRGCSLGADQVSHLAHLNARDTTLAWASRAPQADIARLKERMGWQMPWYTMTDSF
                     DKDFGVDEWHGHNVFFRDGDKVFRTYFINNRGDEAMGSTWSYLDITPLGRQETWEDSP
                     EGYPQTAPYKWWNWHDNYDAEPTPHPKWVDILTDPQGAAIRRREEEETKAS"
BASE COUNT          667 a         1082 c         1147 g          725 t
ORIGIN      
        1 atgcaccccg caattgaagt tcgagagctt acaaagaaat atgaagaagg cacgagcggc
       61 acgctggccc tgcgcggcgt ggatctcgct gtgcaaccgg gcgagttgct gatgctgatg
      121 gggccgtctg gcagcggcaa gacgacgctg ctgtcgatca tgggctgcat cctgacggca
      181 acatcgggca gcgtgcgcgt cgcgggcagg gaagttgtcg ggctgcgcga gaaagatctt
      241 ccggcgctgc gcctggagca catcggcttt gtgtttcagg gattcaatct cttccccacg
      301 ctcacggcgg gcgagaacgt ggagctgatg ctcgacctga aaggcgtttc ggttgccaag
      361 gcgaagacgc gggccgagga actgctcgag caagttgggt tgggagaaaa gtacggcagc
      421 tttccctccg acctgagtgg cgggcagaag cagcgcgtag cgattgcgcg ggcgctggcc
      481 ggagatccgg gaatcattct tgccgacgaa ccgacagcgg cgttggactc gcacaccggc
      541 cgcaaagtca tggagatgat gagcgacttg gcccacaagc gcggacgggc cgtggtggtc
      601 gtgacccacg attcgcgggt gctcagcttt gccgaccgca tcgtgaaaat cgaagacggc
      661 gcagtggcca ccgcgcagga gtctgaaacc gtgccagccg gattaatggc gcatagtctg
      721 cccgaggcag ctctcgagtt ggtttaaccg cagcgttgtt aaagctcgtg tcagtttgaa
      781 atggagtgct tttatgagtc agcgtttttc catcggaagt cttttgaaga gtcgcaccaa
      841 tgttgtgatt atccttggcg ttttcctgtt gacggcatct ctggtgctgg cttcgaggcg
      901 cgcgcacgag tcgacggtcg aggccaaggc cgcgtcctcg agccggatcc ccttgttgat
      961 tgccggtccc ggccgggtcg agccggtttc ggaagacatc aaaatcggtt ccgagcttag
     1021 cggccgcctg aagtcggtgt acgtcgagga aggagacgcg attcaccgcg ggcaggtgct
     1081 ggccgaactg gaaaatgccg actatcgtgc gcaggtcgag tcgtcacgag ctaatgtcgt
     1141 tgcgaaggaa gccacgctgc gcaaagtcat caatggggcg cggcatcagg aacgcgacga
     1201 ggcctggtcc tcggtgaacg aggccaaggc ggtcatggag aacgcgcagt ccgagctgca
     1261 ccggcgccag gaattgtttt cggcgggcgt ggtctcgcgc gaggaactcg atcgctacac
     1321 cagcgacgcc gacgtcgcca aagcgaagta tgagtcggcg gtgcagcatc acgcgctggt
     1381 cgacgaccgg gcgcgcgagg aagaccggtc cttcgccgag gctgatctgc agctggccca
     1441 gggccagctt gccgaagccg aagcccgcta cgagaagaca ttcatccgct cgccgatcga
     1501 cgggacggtg ctgcgcaagc accatcgcag cggagagagc gtgtccaact cttccaccgt
     1561 gcccgatcca atcctgacga tcggcgaccg caagaccctg cgcgtgcgca tggacgtcga
     1621 cgaaaccgac gtcagcaagg tgcgcgtcgg acagaaagct tatgtcaccg ccgatgcctt
     1681 cggcacacag aagttctggg gacgcgtcgt acgtgtgggg cagcagctgg ggccaaagaa
     1741 cgtgcgcacg gacgagccga cagagaaggt cgacacgaag attctggaaa cgctcgtgga
     1801 gctcgatccg ggctcacagc tgccggatgg gctgagagtt gacgcattca tcgtgccgga
     1861 cggtggggaa ctggcgcaga atcgctgagc ccgtcgtatc gttctccgcg acatccgtaa
     1921 caggcaaaat ccccacttct cgcaaagaac gcgagaagtg gggcacccgg cgataatcct
     1981 aatcactgca agagaaacgc aatcgatggg ggtataccta tgactcgcga agccagaatc
     2041 atggctggaa ttattctgat cacggtgccg tcgatccagt atggcggata tttcctgctc
     2101 acgtcgctga tggatcgaac cagccgctac atggataatc cgctgcggca gaattttttc
     2161 cgcgccggtc acgcccacgc gggagtattc gtgattctgg ctttgctctg ccagattctg
     2221 gccgatgcgg cgaacctgcc gactccgtgg ctctggctgg cgcggatcgg cgttccgttg
     2281 gcggcgattc tggtgccgct ggggtttttc ctgtcagtgc cggcaccgtc ttccgtccag
     2341 cccaacggat tcgtggcgtt catctatgta ggagctgtgt tgcttgccgc ctcggtgctg
     2401 acattgggca ttggactgct ccgatcacaa gccgtttgag acgccgcggt acccgcacga
     2461 aatcaaattc cccacttccc gcccagaacg cgggaagcgg ggcacccacc gaatcctatg
     2521 gagagttgcg ctcgacctta ctggcgttct ccagccagtc tgccggcgag accaccttgg
     2581 ccgctgtggg atgttcgctc gttgccggag acatgatccc tgcttctttg gatcgaatcg
     2641 cccggtttgc gtttcttctt gttgccaaga actctcacgg ccagcgcggt gattccgctg
     2701 gtcgacaccg taccgcccac cacccacgcg gccgcgctcg ccacacatac tggacacatg
     2761 atgtcctcca atctctcgac tcgcccgctt cttcccgggc gtgtccttcc ttcagctggc
     2821 cttcgtctct tcctcctcgc gcctgcggat cgcggctcct tgagggtcgg tcaagatatc
     2881 gacccacttc gggtgaggcg ttggctcggc gtcgtagttg tcgtgccagt tccaccactt
     2941 gtacggcgca gtctgcgggt aaccctcggg cgagtcttcc caagtctctt ggcggccgag
     3001 cggtgtgatg tcgaggtagc tccaggtgct tcccatcgct tcgtcgccgc ggttgttgat
     3061 gaagtacgtg cggaacacct tgtcgccgtc gcggaagaac acgttatgac cgtgccattc
     3121 gtcgacgccg aagtccttgt cgaagctgtc ggtcatcgtg taccagggca tctgccagcc
     3181 cattcgctct ttcaggcgag cgatatcggc ttgtggcgcg cgtgaggccc aggcgagggt
     3241 agtatcgcgg gcgttcagat gggccaggtg cgagacctgg tcggcgccca gcgagcagcc
     3301 gcgacaggcg tggtcgggcc agccgaacac tccgggctcg aagaacgcgc gatagacgat
     3361 caactgtgga cgcccctcga acaaatcgag caggctcgcc tttcccttgg gcccgacgaa
     3421 ctcgtactgc ttctccaccg ccatccacgg catccggcga cgctcggccg ccagcgcgtc
     3481 acgggagcgc gtcaaagcct tctccttgac caggagttgc tgccgcgcag cctcccagtc
     3541 ctgccgcgac acgatcgggg gtattttcat cgtgagatgc cgtttcggtt gctccgtctg
     3601 cttctctttg cttgttgcca t
//
LOCUS       DASPUL010000312         4101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_200422, whole genome shotgun sequence.
ACCESSION   DASPUL010000312 DASPUL010000000
VERSION     DASPUL010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200422"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VEJ00_07645"
     CDS             <1..216
                     /locus_tag="VEJ00_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01070.1"
                     /translation="ALVPTLIMAAVMLLAAQSSPQSPSAFRRYLAARPLSYWVMTWFL
                     FELVVLTAIGTFFRGPGWSWVWPWQGS"
     gene            241..2292
                     /locus_tag="VEJ00_07650"
     CDS             241..2292
                     /locus_tag="VEJ00_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXX01071.1"
                     /translation="MNDKSRSLWQKLFGDSVRAFGVVSLVFLLSLAIAPAKNFFSEWR
                     HYQHGYLRTIRNRSDANTLRRHFEGGIQQIWLPDLGVVDRCTSCHVGLKEASLVDVPT
                     QPYRAHPVIPHKSEQFGSTICHRGQGAATTVSEAHNSTLAWEQPILPAKYIESSCGQC
                     HRGPLPGTPQLNEGRRVMSRYGCVHCHTVKLPDGSTMKASDDPPSLSHIADKTTREWI
                     YAWLKDPQAYAATATMPNFKLSDDDARDMSAFLVASSTPVPGDTTTASAKASSDPAAG
                     SSLYGESFCASCHAVQNAAGNLVGGDVGPELTRIGSKVKPEWLQAWLRNPRIYDAGTA
                     MPHYRFNDGQVATLNGFLLAKTDSDLLANVHLEPATPEQIARGKRLVSDYGCASCHEI
                     AGIKKPENFAPDLSSTTQRIGSKPITQLIFLPGMPHTLPDYIAGKIRQPRAFSPGLKM
                     PQYAFTPAQIDALTTALLALNDRSATLPPSLAVPATPESDYQPAGKAGKLMTDLACFS
                     CHRINGHGGDMAPDLTWEGSSVQRDWLVQFFKNPGTLRPALIRRMPRFNLADGEINEL
                     TDYIMTVYQNPVIDRDSMPLSGYPQGEVELGKQLFYGKYSCQGCHIVDTKADKGYIGP
                     TLTHVGSRLTAAWIYQWMKNPQALRPGTVEPNRAMSDEDARAITAFLMSQKGGQEVAR
                     K"
     gene            2289..3032
                     /locus_tag="VEJ00_07655"
     CDS             2289..3032
                     /locus_tag="VEJ00_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXX01072.1"
                     /translation="MKPPTRLTLTLALTLAAGLAASCSHRGESAPLPNPTAFGAAIVE
                     SSGGKQIAQTGSALPQPLVVQVNDEQGTAVLGALVQFTAAPGVDFDPPNGLTDSSGQV
                     TTNVSLGSMAGRYQITASTFDKSHKKVELKVQEIALAYQQTLGRRLNDQYCDRCHNAE
                     STVERVSNYDNLEIKPHPFTEGDTLNKIGDADLTAIITYGGPALNKSALMPAWGNTLS
                     KSDIQALISYIRAIADPPSRGAGPVYAKN"
     gene            3488..3757
                     /locus_tag="VEJ00_07660"
     CDS             3488..3757
                     /locus_tag="VEJ00_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01073.1"
                     /translation="MARAFAHLAGAIAMLLTSLLWVALLHGGKADRFLDDLAGAQNEY
                     KVVLGTALLAVILAFVAAIRADRRWFLGVAFSVGTLGFFTYALSR"
BASE COUNT          832 a         1254 c         1255 g          760 t
ORIGIN      
        1 gcgcttgtgc cgaccctgat catggcggcg gtcatgctgc tggcggccca gagttccccg
       61 caatcaccct ccgcttttcg gcggtatctc gcggccaggc cgctttccta ctgggtgatg
      121 acttggttct tattcgaact ggtggtgcta accgccatcg gtacattttt ccgcgggccg
      181 ggctggagct gggtttggcc gtggcagggt tcataacatg ttcataagga tgcggcgatc
      241 atgaacgaca aatcccggtc gctctggcaa aaactcttcg gagacagcgt gcgcgcgttt
      301 ggcgtggtca gcctggtttt tctgctttca ctcgcgatcg caccagcgaa aaacttcttc
      361 agcgagtggc gccactacca gcatggctac ctccggacga tccgcaaccg cagcgatgcc
      421 aacacgctgc ggcggcactt cgagggtggc attcagcaga tctggctgcc ggatctgggc
      481 gtggtggacc gctgcacgag ctgccacgtc ggattgaaag aagcgagcct ggttgatgtt
      541 ccgacgcagc cttatcgcgc gcaccccgtc attccgcaca agagcgagca gttcggaagc
      601 acgatctgcc atcgcgggca gggcgccgct acgacggtca gcgaagcaca caacagcacg
      661 ctggcgtggg agcagccgat tctccccgcg aaatacatcg agtcgtcgtg tgggcagtgc
      721 catcgcggcc cgctgccggg cacgccgcag ctcaacgagg ggcgcagggt gatgtcccgc
      781 tacggctgcg tgcattgcca caccgtgaaa ttgccggacg gcagcacgat gaaagcgtcg
      841 gacgacccgc cgtcgctctc gcacatcgcg gataaaacaa ctcgcgaatg gatctacgcg
      901 tggctgaagg atccgcaggc ttacgccgcg acggcgacga tgccgaattt caagctaagc
      961 gacgatgacg cgcgcgacat gtcggcgttt ctcgtcgcga gcagcacgcc ggtgccaggg
     1021 gacaccacga cggcttcggc gaaggcgtct tccgatccgg cggcgggcag cagcctctac
     1081 ggggagtcgt tctgcgcctc gtgccacgct gttcagaacg cggcgggaaa cctggtcgga
     1141 ggcgatgtag gcccggagct gacccgcatc ggcagcaagg ttaagcctga atggctgcag
     1201 gcttggttgc gcaacccgcg catttacgat gccgggaccg ccatgccgca ctatcgattc
     1261 aacgacggac aagtagcgac gctgaacggc ttcctcctgg ccaagaccga ttctgatctg
     1321 ctcgccaatg tgcatctgga accggcaacg cccgagcaga ttgcccgtgg caagcgcctg
     1381 gtctcggact acggctgcgc ctcatgtcac gagatcgcag gcatcaagaa gccggaaaac
     1441 tttgcccccg atctcagttc gaccacgcag cgcatcggca gcaaaccgat cacgcagctg
     1501 atttttctgc ccggaatgcc gcacactttg ccggactaca ttgcgggaaa gatcaggcag
     1561 ccgcgtgcat tctcgccagg cctgaagatg ccgcaatacg ccttcacgcc ggcgcagatc
     1621 gatgcactca cgaccgcgct tctggcgctg aatgatcgct cggccacgct gccgccttcg
     1681 ctggccgttc cggcaacgcc tgagtcggac tatcagcccg ccggcaaagc agggaagctg
     1741 atgacggatc ttgcctgttt cagttgccat cgcatcaacg ggcacggcgg cgacatggcg
     1801 ccagatctca cctgggaggg cagctcggtg cagcgcgact ggctggtgca gttcttcaag
     1861 aatcccggga cgctgcgccc ggcgctgatc cggcgcatgc cccgtttcaa ccttgccgac
     1921 ggcgaaatca acgaactcac cgactacatc atgaccgtct atcagaatcc cgtgatcgac
     1981 cgcgattcca tgccgctgag cggctatccg caaggagagg tggagctggg caagcagctt
     2041 ttctacggga aatactcctg ccagggctgc catatcgtcg atacgaaggc ggacaaggga
     2101 tacatcggac cgacgctgac tcacgttggc tcgcgcctca ccgcagcatg gatttatcag
     2161 tggatgaaaa atccgcaggc gttgcgaccg ggcaccgtcg agccgaaccg agccatgagt
     2221 gacgaagatg cccgcgccat aaccgcgttc ctgatgtcgc agaagggcgg acaggaggtt
     2281 gccaggaaat gaagcccccc acacgcttga ccttgacgct ggcgttgacc ctggccgcag
     2341 gactggccgc gtcttgctcg caccgcggcg aatccgcgcc gctgcccaat cccaccgcat
     2401 tcggagcggc cattgtggaa tcgagcggag gaaagcagat cgcgcaaacc ggctctgctc
     2461 tgccccagcc gctggtggtt caagtgaatg acgagcaggg caccgctgtc ctgggagctc
     2521 tggttcagtt caccgcggct ccgggagtgg acttcgatcc tcccaacggc ctcacggact
     2581 caagtgggca agtgacgacc aacgtctcgc tgggaagcat ggccggccgc taccagatca
     2641 ccgcctctac gttcgacaaa tcgcacaaga aagtcgaact gaaagtgcag gagatcgctc
     2701 tcgcttacca gcagacgctc ggccgccggc tcaatgatca gtactgcgac cgatgccaca
     2761 atgccgaatc gaccgtcgag cgcgtttcga attacgacaa cctggaaatc aagccgcatc
     2821 ctttcacgga gggcgatacc ctgaacaaga taggtgatgc cgatctgact gcgatcatca
     2881 cgtatggagg gccggcactg aacaaatcgg cgctcatgcc ggcgtggggc aacacgctga
     2941 gcaagtctga catccaggcg ttgatctcct atatccgcgc tatcgccgat ccgccgtccc
     3001 gtggcgccgg tcctgtttac gcgaagaact agtttgtatc aggcgagggc cggcctttgt
     3061 cccggccgtc actagaactg gaccaactat tgaaaccaga tgggatgaga aggccggtct
     3121 ccggacggga tgcaatgtac gctcccaggt ctggagggca cggacataat gtgctaccac
     3181 gtaggggccg gtgagtgacg gcaaagatct gtccgctagt atcgccaagc gtcaggtcag
     3241 tgatctggtc cttgctgctg cggtcacgat ttcgaccaac ccgacggtgc ctttcacgct
     3301 agcgagctgc caagccgatc aaaagttact cggggcgcgc cgtcatcgcg gggatccaat
     3361 caacgtcaca ggcagtaagg ctcgtcgaat cttcggcgtc gtcacaatgg actgccaggt
     3421 aagataggcc agcaatttgg ctcagcatcc gtttgaggtc cccgaaactc ggcgtacgat
     3481 ctagggcatg gcccgcgcct tcgcccacct cgcaggtgca atcgcaatgc tgctgacgag
     3541 cctgctctgg gtcgccttgc tgcatggtgg taaagccgat cgctttctcg acgatcttgc
     3601 cggagcacag aacgagtaca aggtcgtcct gggcaccgcg ttgctggctg tgatcctcgc
     3661 attcgtggcc gcgatccgag cagacaggag atggtttctc ggggttgcgt tctcggtggg
     3721 gacactaggg ttcttcacat acgcgctttc gcggtgaaga tttgattttt ctggggtacc
     3781 ggccaaagag aaacgacagc cacctttaac cgcgaaaccg cccagggccc cccgacttgg
     3841 accgcccgcg cgcgtggggc gagtgcgata ttctgaaagc aggtcttagg aaaggtcctg
     3901 tggccaagta ccgatagttg gttctcgaga ggttcaactt aaggcgggga tagcagagtg
     3961 atcggcaaag acttagcgac tgacctcggc ctggggagta cgggtctcga taggcctacg
     4021 ccaacagcgt cggtccgagc ggtcgagggg aaaggtcacc ggtccgagga tgaggacaaa
     4081 tcgcgccgtc gcagcgtccg c
//
LOCUS       DASPUL010000313         4329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_328606, whole genome shotgun sequence.
ACCESSION   DASPUL010000313 DASPUL010000000
VERSION     DASPUL010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328606"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VEJ00_07665"
     CDS             <1..1148
                     /locus_tag="VEJ00_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXX01074.1"
                     /translation="EPSYPAKSEDDAAAMCYTSGTTGNPKGVLYSHRALALHSLGISL
                     PDQFDISCHDTVLYAMSMFHANAWGLPYAATMNGCKHVMPGRHLQPEPLLDLLQNERV
                     TLTGAVPTVWLAVINALEAHPGRWKLADGMRIISAGSAVPESLFRRFDKFGVRVIQPW
                     GMTETTPIATCSVPKPHMRDWPEDELYPLRAKQGTTLPFIEIRTMLPEGEAPHDGESA
                     GELEIRGAWVTASYYRLDQSERWTADGWFRTGDVATIDAEGYVKITDRVKDMIKSGGE
                     WISSIDVENALVGHAAVREAAVIAVPHPKWAERPLAAIVLKQGSNVTESELRAFLEKR
                     FARWQVPDAFVFVEELPHTSTGKLLKTKLRKDFANWNWESGPASRLS"
     gene            complement(1163..1882)
                     /locus_tag="VEJ00_07670"
     CDS             complement(1163..1882)
                     /locus_tag="VEJ00_07670"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXX01075.1"
                     /translation="MASVTQNFMKLFRGSSSGPEAAVQPPPQKLSRRSSGLGELSRLW
                     ESDTPLCVLDLGSTSASNIRFFTGRSHRIYSEDLLVASTDPSLITKDEEGKTVLDSRK
                     FLADNLVYPAAHFDVVLCWNLPDYLDESLVRPVMGRLWSVLKPGGMLLAFFHTKDAGP
                     DSPCYRFHIMGKDSLEMQRIVLKREARRGPTGAVHTAVTDGFRLQRVFNNRHIETLFR
                     DFASIKFFLARDNIREVLVVR"
     gene            complement(1914..2273)
                     /locus_tag="VEJ00_07675"
     CDS             complement(1914..2273)
                     /locus_tag="VEJ00_07675"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXX01076.1"
                     /translation="MSAPLSGDLAQIGKSVVIKGELSGSEDLYVDGQVEGSVSLKGNS
                     LTVGPNGQVKASVEARGVVVQGKLEGNVQASDRVELRKSAMVTGDITTQRISIEEGAY
                     LKGKVDIQGKAEKAAGK"
     gene            2504..3436
                     /locus_tag="VEJ00_07680"
     CDS             2504..3436
                     /locus_tag="VEJ00_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01077.1"
                     /translation="MCRISRQRRGAALAGACLALALGASAVTAAATQASSRPAMAAGE
                     LVRLAVANEVAAANDTSAKHMFRSRRQTPKGSQTRLYVETDDAIAAMLISDNDRPLTL
                     QQQQAEANHLAWLMNNPDQLRKKHAREREDADRTLRIVKALPDAFSYEYAGTDNGSGG
                     TEAGPQLARLNFRPNPSYSPPSRVEQVLQGMQGYLLIDTNARRIARIDGTLFRAVTFG
                     WGIIGHLDKGGHFLVRQADLGDGTWDITEMDLSITGKILFFKSISMVSDEVFSDFRRV
                     PDNLTFAQGVELLKSEREKLARSEAPSEAKRTPQ"
     gene            complement(3437..>4329)
                     /gene="menC"
                     /locus_tag="VEJ00_07685"
     CDS             complement(3437..>4329)
                     /gene="menC"
                     /locus_tag="VEJ00_07685"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HXX01078.1"
                     /translation="TLLGRRLESARECPALLTRVRSHRMAKAALENALWDAEAIQKQQ
                     PLWKLLGGVRSQIPCGVSIGIQDSVEQLLEKIQTELAAGYRRIKVKVKPGWDVNVLER
                     IRALWADITLSCDANSAYTLDQVEHLRKFDQFNLLMIEQPLWHDDIYYHARLQKELRT
                     AICLDESIISARTAAFAVESAACRIINVKVGRVGGFTEAMKVHDIARDNNIPVWCGGM
                     LETGIGRAHNIALSTLPNFSLPGDVSASKRYWKEDIIEPDVEVSPQGMIAISEKPGRG
                     YEVKPDLIEKLTVRKETIRA"
BASE COUNT          893 a         1295 c         1327 g          814 t
ORIGIN      
        1 gcgaaccgtc gtatcccgcc aaatccgagg acgacgctgc tgccatgtgc tacacgtcgg
       61 gcacgaccgg caatccgaag ggcgttttgt actcgcaccg ggcgctggcg ctacattctc
      121 tcggcatctc gttgcccgac cagttcgata tcagctgtca cgacacagtc ctctatgcca
      181 tgtcgatgtt tcacgccaac gcctggggac tgccctacgc cgccacgatg aacggctgca
      241 agcacgtcat gccggggcga cacctgcagc ccgagccgct gctcgacctg ctccagaacg
      301 aacgcgtgac gctgaccggc gccgtgccca cggtctggct ggcggtaatc aacgcgctcg
      361 aagctcatcc cggccgctgg aaactggctg acggcatgcg tattatctcg gccggttcag
      421 ccgttcctga atccctgttc cgccgcttcg acaaattcgg ggtgcgcgtt attcaacctt
      481 ggggaatgac ggaaaccacg ccgatcgcga cctgctcggt tcccaagccg cacatgcggg
      541 attggccgga agacgagctt taccccctgc gagcgaaaca aggaacgacg ttgccgttca
      601 ttgaaatccg caccatgctg cccgagggag aagcgcctca cgatggcgag tcggcagggg
      661 aattggagat ccgcggggcc tgggttacgg ccagctacta ccgactcgat caaagcgaac
      721 gatggacagc cgacggctgg tttcgcaccg gcgacgttgc cactatcgat gccgaaggct
      781 atgtcaagat cacggatcgg gtgaaagaca tgatcaagtc gggaggcgag tggatcagtt
      841 cgatcgacgt ggagaatgcc ctcgtcggcc acgccgccgt gagagaagcg gcggtcattg
      901 ccgtgcctca tccgaagtgg gcagagcggc cgctggctgc gatcgttctg aaacagggaa
      961 gcaacgtcac ggagtcggaa ttgcgagctt tcctggagaa gcgcttcgcc cgctggcagg
     1021 tcccggatgc gtttgtgttc gtggaagagc ttccccacac ttcgacggga aagctgctca
     1081 aaaccaagct acgcaaggac tttgccaact ggaactggga atcaggacca gcgtcgcggt
     1141 tgagctgagc gaatgaagat ggctaccgca cgaccagcac ttcgcggata ttgtcgcgcg
     1201 ccaagaagaa cttgatcgag gcgaagtcgc ggaacagagt ttcgatgtgg cggttgttga
     1261 agacgcgctg caggcggaat ccgtcggtga cggcggtgtg gacggctccg gttgggccgc
     1321 gacgggcctc gcgcttgagc acgatgcgct gcatctccag ggaatctttt cccatgatgt
     1381 gaaagcggta gcagggagag tcgggcccgg cgtctttggt gtggaagaag gccaggagca
     1441 tgccgccggg cttcaggacc gaccacagcc tgcccatgac cgggcgcacc agactttcat
     1501 cgagatagtc gggaagattc cagcacaaca caacgtcgaa atgagcggcg ggatagacca
     1561 ggttatcggc cagaaacttg cggctgtcga gaacggtctt gccttcttcg tctttcgtga
     1621 tcaggctcgg atcggtggaa gcgaccaaca ggtcttcgct gtaaatcctg tggctgcgcc
     1681 cggtgaagaa gcggatgttc gaggccgaag tggaaccgag atcgagaacg caaagaggag
     1741 tgtcggattc ccaaagacgg gaaagctctc ccaaaccgct ggagcggcgg ctaagcttct
     1801 gcggcggcgg ttgtaccgcc gcttccggcc cgctcgatga accacgaaag agcttcatga
     1861 agttctgagt gactgatgcc atcaatctaa cctgctcgaa cgcgcaaggg gagtcacttt
     1921 cctgcggcct tctccgcctt gccctggata tcaaccttcc ctttcagata tgcgccttcc
     1981 tcgatggaaa tgcgctgcgt ggtgatgtcg cctgtgacca tggcggactt gcgcagttcg
     2041 acccgatcgc tggcctggac gttgccttcg agcttgccct gaacgaccac accgcgggct
     2101 tcaaccgagg ccttgacctg gccgttgggc cctacggtca ggctattgcc tttcagcgaa
     2161 acgctgcctt ccacctgtcc gtcgacatag aggtcttcgc tgcccgagag ctcacccttg
     2221 atcaccaccg atttgccaat ctgcgccaaa tcaccggaca acggagcact catgaacgtt
     2281 ctccttcagg aagcgggatg ctgcacgacc agggttgcac gaccaggata gctggcaagg
     2341 cagctgctac cgacaccgcg tcacttgctt cgactatacc cactgcagaa gcgcgcggca
     2401 agcggagaac taggccgcac cccgaagccg cggacacgga aggacaccaa agtaaccggc
     2461 gaaatctacg ccgtgccgcg cacattgcta cactgggctt gagatgtgcc gcatttctcg
     2521 acagcggcgc ggtgcagcac tggccggagc ttgcctggcc ctcgcacttg gcgcctcggc
     2581 agtcaccgcc gcagcaacgc aggcatccag ccggccggct atggccgccg gagaactggt
     2641 gcgcctggcg gtggccaacg aagtggcagc ggccaatgac acgtccgcca agcatatgtt
     2701 ccgctcccgc aggcagactc cgaagggatc acagacccgg ctgtacgtgg aaaccgacga
     2761 tgccatcgcg gcgatgctga tctccgacaa cgaccggccg ctgacgctgc aacaacaaca
     2821 ggcggaggcc aaccatctgg cctggctgat gaacaacccc gaccaactcc gcaagaaaca
     2881 cgcgcgagag agggaggacg cggatcggac gctacgcatc gtcaaggcgt tgcccgacgc
     2941 tttcagctac gagtatgcag gcacagacaa cggttcgggc ggaactgaag cgggcccgca
     3001 gctggcgcgg ctgaacttcc gtcccaaccc gtcatattcg ccgccctcgc gcgtagagca
     3061 ggtgctgcaa ggcatgcagg gctacctgtt gattgataca aacgcccggc gcatcgcgag
     3121 aatcgacggg acattgttca gggcggtgac gtttggttgg ggcattatcg gccacctcga
     3181 caaaggtgga cacttcctcg tgcggcaggc ggatctgggc gacggcacat gggacatcac
     3241 cgaaatggat ctcagcatca ccgggaagat cctgttcttc aagagcatca gcatggtttc
     3301 cgatgaagtc ttcagcgatt tccggcgggt acctgacaac ctgactttcg cgcagggagt
     3361 ggagctgctg aaatcggagc gggaaaagct ggcgcgatct gaggctccct cggaagcgaa
     3421 gagaactccg caatagctag gcgcgtatcg tttccttccg cacggtgagt ttctcgatca
     3481 agtctggttt cacttcatag cctcggcccg gcttttcgct gatggcgatc atcccctgcg
     3541 gcgaaacttc cacatccggc tcgatgatgt cttccttcca gtaacgcttt gacgcggaga
     3601 cgtcgcctgg aaggctgaag tttgggaggg tcgaaagagc gatattgtgg gcgcggccga
     3661 ttccggtttc cagcatgcct ccgcaccaga cgggaatgtt gttgtcccgg gcaatgtcgt
     3721 gcaccttcat cgcttccgtg aacccgccga cccggccgac tttgacgttg atgatccggc
     3781 aggcggcgct ttcgacggcg aaggccgcgg tgcgcgcgct gatgatggac tcgtccaggc
     3841 aaatcgcggt gcgcaattcc ttttgcagcc gggcgtggta gtagatgtcg tcatgccaca
     3901 gcggctgctc gatcatcagc aaattaaact gatcgaactt gcgcaggtgc tcgacctgat
     3961 cgagcgtgta ggcggaattg gcatcgcagc tgagcgtgat atcggcccag agcgcgcgaa
     4021 tgcgttccag cacattgaca tcccagccgg gcttgacctt aactttgatg cggcggtatc
     4081 cggcggcgag ctcggtctgg atcttctcca gcaactgctc aactgaatcc tgaataccga
     4141 tggaaacgcc gcagggaatc tgagaacgca ccccgccgag caacttccag agtggttgct
     4201 gcttctggat ggcttcggca tcccacaatg cgttttcgag agcggcttta gccatgcgat
     4261 gcgagcgcac tctcgtgagc aaggcggggc actcgcgcgc ggactccaga cgtcttccaa
     4321 gaagcgtgg
//
LOCUS       DASPUL010000314         4467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1835774, whole genome shotgun sequence.
ACCESSION   DASPUL010000314 DASPUL010000000
VERSION     DASPUL010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835774"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VEJ00_07690"
     CDS             complement(<1..222)
                     /locus_tag="VEJ00_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01079.1"
                     /translation="MDNDVLPEITPARPAQQPGGTQHAQQFERLLEECRELTRDRLSQ
                     SLSGMLDKAADALWEMHKATQDRDTQKLYQ"
     gene            complement(399..1013)
                     /locus_tag="VEJ00_07695"
     CDS             complement(399..1013)
                     /locus_tag="VEJ00_07695"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HXX01080.1"
                     /translation="MAAWRVEGQYMETCNCTYLCPCISSNLAARPTEGDCKVAIAMKI
                     DKGEKDGVKLDGLSFIVLMHAPKAMGEGGITVGLIVDERANDKQTEAISAIATGAAGG
                     PMAALGPLVGRVAGMEKRPISFDAQGLKRTVKAGKLVDQSCEGVPSPARAGEPIYLDN
                     TLHPVNSRLALAKATRTSFHAFGIDWDDSSGTRNGHFAPFAWSG"
     gene            1277..2674
                     /locus_tag="VEJ00_07700"
     CDS             1277..2674
                     /locus_tag="VEJ00_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01081.1"
                     /translation="MRVLYWKLVSAAVSTVVILLASSNNALAVPSFARQTGMACEACH
                     TVFPELTPFGRAFKLNGYLIDNLPQVKEVTAEKKESLLLNWLPPLSVQFVASYTKFNT
                     ALPDPNGGLSQNGTVQFPEAFSLFYAGRIAPQLGGFIQITYDNNADAIGWDNTDIRFA
                     DLTTLGQDKPLTWGVSANNSPSVQDPWNSLPAWQYPFSQTSSLAVQPVATTQLEGIGN
                     GTVAGLTGYGYWNNLVYLELGGYAPAPHLLGGVPLNSSFCTTANGCPTEVVHGFSPYW
                     RVAVQPQWGRNSFEVGLVGFSDQIVPPDTNVTTGGPLNKFRDTGLDAQYQYIGDDHIF
                     SAQARYIYEKQTLNAAAANAAAGGNPGFDPNNTLKSLRIGGSYFYQRQLGGSIGYFST
                     MGNTNPALYATTTGSPNTNGWAFEADYLPWQNVKLAVQYILYTKFNGSSSNYDGTGRN
                     ASDNNTLYLFAWIAF"
     gene            2730..3338
                     /locus_tag="VEJ00_07705"
     CDS             2730..3338
                     /locus_tag="VEJ00_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXX01082.1"
                     /translation="MKLGVLGGVVLALSLVASIAYSAEDQEIKRYTAQVCAACHGPKG
                     NSTSPAFPRLAGQQAAYIEAQLTGFHDRTRGDPAAQAYMWGMASQLDARDIKQLAAYY
                     AAQQPVPGTPGDKTLMAQGKTIYEQGMADAGIPACIACHGAGAEGKEAIPRLAGQHPA
                     YLVKQLAYFKAQLRANAPVMHAVTDKMTFEQMEAVATYAASK"
     gene            3370..3810
                     /gene="bamE"
                     /locus_tag="VEJ00_07710"
     CDS             3370..3810
                     /gene="bamE"
                     /locus_tag="VEJ00_07710"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HXX01083.1"
                     /translation="MGLAAALLPAHAQEQDPASLQKEVDQLRQEVQSLKEQVKSLQEA
                     PPAATPPQTQPAVTQPAPNQVEPAPAKPPAGSITALRESWSHVGKGMTKAQIEEVLGP
                     PTNELTINGKVVWYYYYAGIGGSSVFFNGDGRVSSHQRPTVGWW"
     gene            complement(3783..>4467)
                     /locus_tag="VEJ00_07715"
     CDS             complement(3783..>4467)
                     /locus_tag="VEJ00_07715"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HXX01084.1"
                     /translation="GEPMQWRVKIGTDETAAVLEAASTGSKGRLFVCAHGAGGHMADR
                     GMLALSEEFHRRGTDVVRFNFLYREKGSRKPDAMPRLEECVSAVVSRARQEVQPDLLF
                     IGGRSMGGRAASMLAAEGFSCDGLILLAYPLHPAGQPEKLRSTHLPQIKVPVLCLNGT
                     RDSLCTRELMDRVVGGLSRSWTMHWLEGADHSFHVLKSSGRSDGDVLNEIGDGYSAWE
                     AHHHPTVGR"
BASE COUNT          893 a         1352 c         1363 g          859 t
ORIGIN      
        1 ctggtacagc ttttgggtgt cgcgatcctg cgtcgccttg tgcatctccc agagggcgtc
       61 ggccgctttg tcgagcattc ccgagagaga ttgcgagagg cgatcgcggg tgagctcgcg
      121 acactcctcc agcaggcgct cgaactgctg ggcatgctgg gtcccaccgg gctgttgcgc
      181 cgggcgggct ggggtaatct cagggaggac gtcgttgtcc ataagctcaa ccccgcagct
      241 atgcaagttg cgggccttac gaaccggcac gcccggaaga cgctggtttc ctcggatccg
      301 aggcggatca gcgacgattg gtagcgaaaa cgggcttggc cgcgctcgcc agcgccggcc
      361 ccaggcgtcc ggaggccttg tcgacgcggc tgcgggcgct agccgctcca cgcgaacggt
      421 gcgaagtggc cgttgcgtgt gccgctcgaa tcgtcccaat cgatcccgaa ggcgtggaag
      481 ctcgtgcggg tggctttggc gagcgcgagc ctggaattga ccgggtgaag ggtgttgtcg
      541 aggtagatgg gctcgcctgc tcgtgcgggg ctggggacgc cttcgcaact ttgatcgacg
      601 agcttgccgg ctttgactgt gcgcttcagt ccctgcgcat cgaagctgat cgggcgcttc
      661 tccatcccgg ctacgcgtcc caccaacggg ccgagcgctg ccatcggtcc accggccgcg
      721 ccggttgcta tcgcgctgat cgcctcggtc tgcttgtcgt tggcgcgctc atctacgatc
      781 agccccaccg taatgccgcc ctcgcccatc gccttcggcg cgtgcatcag cacgatgaac
      841 gaaaggccgt cgagcttcac gccgtccttc tcgcccttgt cgattttcat ggcgatggcg
      901 accttgcagt ccccttccgt gggccgggcc gcaaggttgg agctgatgca cggacacaga
      961 taggtgcagt tgcaggtttc catgtactgc ccttcgacac gccaggcggc catagctgca
     1021 ctcctttctg tgagagtcgt ttctgtgagg gtagatctgc gcgcatcgag cagaagcgct
     1081 cgaacgagag tacgcctgcg acccggtccc cgcaagcccc gggatgatgt tcaggacgat
     1141 ggcattaatg cgtctttttt tcgtctgtca ggttgcttac gcatacctta ctactcgaag
     1201 ttgatctaag tcaaccgcac acagagcaat cgagcgcgta gtgggaacgc ataagcgcga
     1261 tgggagggaa taagcgatgc gcgttcttta ctggaagctc gtcagtgcgg ctgtcagcac
     1321 ggtagtgata ctgcttgcaa gttccaacaa tgcgctggcg gtacccagct tcgcgcgaca
     1381 gaccggcatg gcatgcgaag cgtgccacac ggtgtttcca gagctcacac cgtttgggcg
     1441 cgctttcaag ctgaacggct atctcatcga caatctgccg caagtcaagg aggtgacggc
     1501 cgagaaaaag gagtctttgc tgctgaactg gctgccgccc ttgtcggtgc agttcgttgc
     1561 ctcgtacacg aaattcaata cggcgctgcc cgacccgaat ggcggcttgt cgcagaacgg
     1621 cacagtccag tttcccgaag cgttcagcct gttctatgca gggcggattg cgcctcagtt
     1681 gggtggcttc attcagatca cgtacgacaa caacgccgac gcgatcggat gggacaacac
     1741 cgacatccgt ttcgcggatt tgacgactct ggggcaagac aagcctttga cgtggggcgt
     1801 aagcgccaac aacagtccca gcgtgcagga tccgtggaat agtctccccg cctggcaata
     1861 tcctttctcg cagacgagca gcctcgccgt gcagccagtc gccacgactc agctagaggg
     1921 aatcggcaac ggcacggtgg cgggtctgac cggctacggg tactggaaca atcttgtgta
     1981 cttggaactc ggtgggtacg ccccagcccc tcatttgtta ggcggcgtgc cgttgaatag
     2041 ttcgttctgc acaacagcaa acgggtgtcc cactgaggtt gtgcacggct tttcaccata
     2101 ctggcgcgtt gcagtgcagc cacaatgggg gcgtaactct tttgaggtag gtttggtggg
     2161 cttcagcgac cagatcgtcc cgccagacac caacgtgacg acaggcggcc ctctaaataa
     2221 attcagggac acagggctcg acgcgcaata tcagtacatc ggtgacgacc atatcttctc
     2281 cgctcaagcc cggtacattt atgagaagca aacattgaac gcggcggctg cgaacgcagc
     2341 agctggggga aacccgggct tcgaccccaa taacacgctg aagagccttc ggattggagg
     2401 cagttatttc tatcagcgtc agttgggcgg atcgatcggc tacttctcga ctatgggtaa
     2461 tacgaacccc gccctctacg cgaccacaac gggaagtcca aacaccaacg ggtgggcgtt
     2521 cgaggccgac tatctgccgt ggcagaacgt gaagctggcg gtccagtaca tcctctacac
     2581 aaagttcaac ggctcgagca gcaattacga tggcacgggc aggaatgcct cggacaacaa
     2641 cacgttgtac ctctttgcct ggattgcatt ctgaatggag ttatctggct tgtcgcaacc
     2701 agaatggaca aacaaaaggg ggttccaaca tgaaactcgg tgtcttgggc ggggtagtgc
     2761 tggcgctttc gcttgttgca tcgatcgcgt actccgctga agatcaggag atcaagcgtt
     2821 acacagcgca ggtgtgtgcc gcgtgccatg ggcccaaggg gaacagcacg tcgcccgcct
     2881 ttccgcggct cgcgggacag caggccgctt acatcgaagc gcagctgacg ggattccacg
     2941 atcggacgcg cggcgacccc gccgctcagg cgtacatgtg gggaatggcg tcacagctgg
     3001 acgctcggga catcaagcag ctcgcggcat actatgcagc tcagcagcct gtaccgggta
     3061 cgcctggcga caagacgctc atggcgcaag gcaaaacaat ctatgaacag gggatggccg
     3121 acgctggcat accagcttgc atcgcttgcc acggtgccgg ggcggagggc aaagaagcga
     3181 ttccacgcct tgccggacaa catccggcgt atctcgtgaa gcagcttgcc tatttcaagg
     3241 cacagcttcg ggcgaacgcg ccggtgatgc acgctgttac cgacaagatg acgttcgagc
     3301 agatggaggc ggtagcgacc tatgcggcat ccaagtagca gattggagca gctcagcgca
     3361 gcacttttcg tgggacttgc cgctgcgttg ctccccgcgc acgctcagga gcaggacccc
     3421 gcgtcgctcc agaaggaagt cgaccagctg cggcaagagg tgcagagctt gaaggagcaa
     3481 gtgaaatccc tccaggaagc gccgcccgcc gccacgccgc cgcagaccca accggcggtc
     3541 acacaaccgg caccgaatca agtcgagccc gctcctgcca aaccgcccgc tggatcgatt
     3601 accgcactaa gggaaagctg gagccacgtg gggaaaggca tgacaaaagc gcagatcgaa
     3661 gaggtgctcg gtccacccac gaatgaactg acgataaacg gcaaggtcgt ctggtattac
     3721 tactacgccg gcatcggagg ctcatccgta ttcttcaacg gcgacggccg cgtttcgagc
     3781 catcagcggc cgacggttgg atggtggtga gcctcccacg cgctgtaccc atcaccgatc
     3841 tcgttcagga cgtcgccatc cgatctgcct gagctcttca atacgtggaa ggaatggtcg
     3901 gctccttcca gccagtgcat ggtccagctt ctggacagtc cgccgaccac tcgatccatg
     3961 agctcgcggg tgcacaggct gtcgcgggtg ccattcaggc ataggaccgg gactttgatc
     4021 tgcggcaggt gcgtactgcg caacttctct ggctgcccgg ccggatggag cgggtaggcg
     4081 agcaggatga gcccatcgca cgaaaacccc tcggccgcca gcatcgaagc tgctctcccg
     4141 cccatcgatc ttcctccgat gaaaagcagg tcgggctgca cctcttggcg cgcccgtgac
     4201 acgacggccg aaacgcattc ctcgaggcgc ggcatcgcat cgggtttgcg cgagcccttt
     4261 tcccgataga ggaaattgaa gcggacgaca tcggttccgc gccgatgaaa ctcttcggag
     4321 agcgcgagca tgcctcgatc cgccatgtga ccgccggccc cgtgggcaca gacgaacagt
     4381 cgtcctttgc tccccgtgga agcggcttcc aagaccgcag cagtctcgtc agtcccgatc
     4441 ttcacccgcc actgcatcgg ctcaccg
//
LOCUS       DASPUL010000315        20785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_328779, whole genome shotgun sequence.
ACCESSION   DASPUL010000315 DASPUL010000000
VERSION     DASPUL010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 20785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20785)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20785
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328779"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1644
                     /locus_tag="VEJ00_07720"
     CDS             61..1644
                     /locus_tag="VEJ00_07720"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXX01085.1"
                     /translation="MEVIEAIYQFRTAEGRCLGVLRFPAAQPDKAWTMSTSLRELKGF
                     EEPVGGRRPDGTTARIFGGEPWSRRRAREQRYDDREPAVLIVGGGHNGLVLAARLRML
                     GVDSLVIERMPKVGDVWRNRYDALALHNEIVLNHMPYLPFPTSWPKYLPKDVLGDWLE
                     AYAMALECNVWTETNFTEGRYDAAKGVWNARVRRSDGSERVLHPRHLVFANGIVGEPK
                     LPEVPGLRDFKGTVVHAQGFDSGARWRGKNALVLGVGNSAHDIAQDLHGHGANVKMIQ
                     RGSICVFSVKAASINHAIYYKEGLSLEDADLIASSNTFAVLLRGYQLSTQRMLEIDKE
                     LLARLHAIGFKLDIGPEGGGHQMHVRKNHGGYYLNVGCSDLLADGEVGLLHYEDIDRF
                     IPEGALMKDGHVEKADIVVAATGYQDPTDVVRSLLGDEIAEKIGPVWGMDQGGEMCNM
                     YKPTPQKGLWFIGGGFAQSRVWSHYVALQIKAREAGIVADTPIEKPKLDTLTGPPRPY
                     REAALPADDTGAGAGTDGL"
     gene            1687..2226
                     /locus_tag="VEJ00_07725"
     CDS             1687..2226
                     /locus_tag="VEJ00_07725"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXX01086.1"
                     /translation="MRIPYWSPELKRQPDDLVKAILDRRGGSLINLDRALLWSEPLAR
                     GWNTYLKAVRSELGTSRRLRELGICAVALITGADYEYEHHAPDYLAAGGSQASLDALA
                     AYVKSGVNRDPHGLSGDEALVAQYAAQMTRNVQVDDGLFDRMKDRFDTTQLVEITAAI
                     ATYNMVARFLVALDVTPEA"
     gene            2260..3063
                     /locus_tag="VEJ00_07730"
     CDS             2260..3063
                     /locus_tag="VEJ00_07730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX01087.1"
                     /translation="MHRLKDKVAIIIGAGQSPGEAIGNGRATTIRFAQEGASVLAVDR
                     NLASARESLEMAGTAVDGEAFEVDVARSEMLQAAVDAAMARWGRIDILHYNVGVSVMG
                     GEQTLDKLTDEVFDLINAINLRGAVMAAKFVQPIMRKQGSGVLINVASISAIETFTHL
                     VAYRTSKAGMVAFTRQFAIENAEYGIRANTILPGLMETAMAVDTRMRLSGRSREDIVA
                     ERNSKIPLRRGGSGWDVANAALYLASDEASFVTGIDLPVDGGTLARIGW"
     gene            complement(3087..4526)
                     /locus_tag="VEJ00_07735"
     CDS             complement(3087..4526)
                     /locus_tag="VEJ00_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXX01088.1"
                     /translation="MTLVEQFAGFAASADFDTLPSDVVIESKRLLLDAVGCALGGLSH
                     PKGAIGVEYARRQGPGAPADQASIIGTGDKVACVAAGFANAELINALDFDALLPPGHV
                     SPYVIPGALAAAEASGASGKDLLCGIAVAHELSHRMGKGLDYLRDIKDGKVSPPPVFG
                     YSSTIFGAAAAVGRVRRFSPQMTTDAIGIAASMSPVNAQWSWSVHLPTATVKYAVAGA
                     LVQTAMTGATLAQLGHTGDRQLLDDPVSGWPKMISSSRWDPASVTPGLGEVWLFPAKV
                     TYKSYPHCGALHGPIDVVRDIVQSNRIRPSEIENIKAWVEAHVIQPLWLNRNIEHVTQ
                     AQFSIAHGLSVAAHLIPPDKAWQSPEVVFDPSVLALMDKIEVEAHPDYGKLLTSDPAS
                     RPARVEVRARGETYTGEKRYPKGGRSPDPSTTVSNEELFEKFRRNATGVLSADGIETA
                     TAMIWSLERLADLGELMHNIHPNCSSTYR"
     gene            4847..5824
                     /locus_tag="VEJ00_07740"
     CDS             4847..5824
                     /locus_tag="VEJ00_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXX01089.1"
                     /translation="MNILKAMLRMLFVFVAFAASPVTAWAQQYPNHPIKLIVPFAPGG
                     NTDVAGRLIADGIGQGLGQTIIVENKPGAGASIGAELAAKAPPDGYTLLLASDAQNIN
                     PAIYTTLHHDIIKDFEPVGLAMVTTLVLVASNSSGIHSVADLITEAKAKPGKVTYSSA
                     GVGSGSHMAGALFDQAANIKTLHVPYKGSGPATTAVVAGEVDITYTSLAATVPFYKEK
                     KLQALAITGKDPSPLFPGVPTTESLGFKGYEAGDHIGLLAPKGTPKNVIDRLNTELNK
                     WLSKPETKARLEQLGFTPRSSTPAEYGAFIRSEVAKWDKTAKTGNIKVE"
     gene            complement(5935..6924)
                     /locus_tag="VEJ00_07745"
     CDS             complement(5935..6924)
                     /locus_tag="VEJ00_07745"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXX01090.1"
                     /translation="MARLRSRRSFLANTAAIGAAFGLGKAAFAQATFPDKSKPMRGIV
                     PLAAGSTVDALARIYAQQMGEILGVTFIIENRPGGEFLIGIQAVKSAPADGYTILFSS
                     ISSQVVNPHLFKQLPYDPLKDFIPLGGTMKTPLVMIAGPEFPLKTVKEVVAAAKRDPE
                     KYSYGSVSATTRVAGGMFAQATGIKLLNVPYKNFGDMISDTLTNRINFFFADGAALLP
                     YQVKGMRGLAVCAKTRSLSFPDVPTMTEAGVPLEIVGYQSAYVAAGTPPAVVAVLLDA
                     LKKAESSKAVKDFIASTGNEVMNLHGEQFAAYERSEYERWGQAVREAGLAGSL"
     gene            complement(6974..7972)
                     /locus_tag="VEJ00_07750"
     CDS             complement(6974..7972)
                     /locus_tag="VEJ00_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXX01091.1"
                     /translation="MAFVEVDDPTPGSGEVVVEIKASGMCGSDLHPYRAPNNPDIPLT
                     GRFIGGHEPAGVVFATGPGLDRHVAKPGDRVMVHHYHGCGVCGHCRTGWPQLCRPPMR
                     TTYSGNAHGAHAPYMKVAASTLVPLHEALSFEAGAAIACGTGTAWGALERLQVRGDET
                     LAVFGQGPVGLSTTMLAAAQGARVVAIDIDRDRLAIAASFGAHNLVNPLEADPVQALR
                     DLTHGRGVECVMETSGSSDAASAGLAALAVWGRMCMVGIGASVNLIMQALLDRQITVM
                     TSYTMSTVSQQECADFVVHRALDIDRLFTHRWRLGQAEEAYRLFDTQSTGKGAFVF"
     gene            8435..9481
                     /locus_tag="VEJ00_07755"
     CDS             8435..9481
                     /locus_tag="VEJ00_07755"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXX01092.1"
                     /translation="MMKKWMADMRVDRSGNVPQIARRAARYAGLYSLAATAALFVLMA
                     SNAMSQEPTLPPLIRIVVPFPPGGSNDIAARQVAKQLASRTGKNVIVENRAGASGFIG
                     ASVVARSPKDGSVLLLTSSSTVSAAATKNNVTVDFLKDLMPVSILSESPLMIVVSSES
                     KIQTPGDLIAAARAKPDTLTNGTPGNGTIGHLTSELLNDAAGIQIKHIPYTGGGPAMV
                     DLLANRIDINIVSNSAAAGYVTQGKLRSVAITAAKPSSDFPGVPTVASVVPGFEVVQW
                     QGVWAPAGTPDAIVDRLNRELNQIVKTPEFAVLLHEDGGIPQSLTPAQAAIKVRDSYE
                     MWRKLVKARNLIVD"
     gene            complement(9580..10332)
                     /locus_tag="VEJ00_07760"
     CDS             complement(9580..10332)
                     /locus_tag="VEJ00_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX01093.1"
                     /translation="MPRPDISVYWVPGCSSCIKAKEFVASLGEPFESLNILEHPAALA
                     EVAAAGIRGAPAVRKGDKIVYAQSLDDLAAFFGVTRVERRLPRSVLVTRWDALQDKGR
                     EVIDTFPEEELEGHKLVGPHANVLELAEHAYQIVESFLRLIEDDTIDARAIYLTPDRS
                     IRSRAALLSYADRMCANYDQWINSGGPSSIPTRLNTHYGNQPADIVLERYVWHAAQHL
                     RQLDHLAAGENRALLIPAALYDGLPLPTALWG"
     gene            10957..11937
                     /locus_tag="VEJ00_07765"
     CDS             10957..11937
                     /locus_tag="VEJ00_07765"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXX01094.1"
                     /translation="MKHTALLRLIAFISLSLFTIASAVAQEYPTRPIKLVVGYGAGGT
                     ADATARIYAEKLQTILKVPVIVDNKPGAYEQIAAQAVLSAPPDGYTLWWGTTGALTMG
                     PGIRTNTPYDVIKNFTQIGKIAEVEAVLAVKKDSPINSLPDLIKFAKANPGKLFYASA
                     GVGSGNHLLTEYIMNQTGIKMEHVPYKSDADVARELAAGTADFGIPVAGVAVPFVKEG
                     KFKAIAVTGMQRLKQLPNVPTMGEEVPALNIGVYAIYGLLAPLGTPAPVVRTLSDAFN
                     KVAEMPDVVSRLEATNVRPATSNPNDFRQYLEAEGKRWRELGKSLNITLN"
     gene            12072..13688
                     /locus_tag="VEJ00_07770"
     CDS             12072..13688
                     /locus_tag="VEJ00_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXX01095.1"
                     /translation="MSSDSNLWDDVVIGAGSAGCIVAARLSENNARRVLLLEAGPRDV
                     WLMKPFGLSYYLDFSRFEWGFWSEPDPTRNMRSDHWRRGRVVGGSGSINGMNYVRGTR
                     ADYDRWAAMGNDGWSANDVMPLFRDMEHCEPGYHTPPDYSIRGDSGPLAIREVRNCHP
                     LTEAFLKAAQAAGIPRTNDFNGAQQEGVGYGQFNQRRGMRVSSVDAWLNPALRRNNLK
                     LLTDALVHRLHVKDGRVTGVAYERDGAMREARAGRVIICGGAINTPQLLMLSGIGDGK
                     ELQSLGIDVVLHRPTVGRNLMEHPLIRPSWRVNKPSYSPTNNMLRMAGFGAKFLFTGQ
                     GPLATITEAMGFLRTSPQEPAPDVHMLFSVAGAVNDNELKFYKSLKVLPYPSFSMVLD
                     KSYPLSRGTIRLASADPKAAPLIHPNLLGDPRDLQTLVRAVGVVRRIVSKSPLADMVV
                     DEVMPGKHAESEQALIEYVKDQTGLAYHASGTCRMGVDEGAVVTPDLKVRGVENLWIA
                     DASVMPDLISGNINAACMMIGEKLGRQLRH"
     gene            complement(13822..14121)
                     /locus_tag="VEJ00_07775"
     CDS             complement(13822..14121)
                     /locus_tag="VEJ00_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXX01096.1"
                     /translation="MPYVIMFVDDPGVIEKKKRVRPVHLEYVLKNAHRIITSGGLFPD
                     DADFPNGGLIILDTDMRQDAIDYIENDPFYLNGIFSTYTVSRWRKFIFDYKPISS"
     gene            14483..15892
                     /locus_tag="VEJ00_07780"
     CDS             14483..15892
                     /locus_tag="VEJ00_07780"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXX01097.1"
                     /translation="MSTSPDLCFHFANHAVATTYDRLPPSAIDIAKKSVVDSLGVILA
                     ASGMEPAVRGVIDLVKESGGRPEASILAFGGRVPAMMAALANGAMAHCLDYDDQTPWG
                     QHSGSSLLPAVFAAAEKKGGVSGKKMIAAVAVGQDIFNRLRCHVEWEKDWVFTTVMGV
                     YSATAAAGAVLGLPRDRIANALGIASMQCSGTMNMIHAVGSDLRAMYAGFPAKGAVLS
                     ALLSEKGVTGVPDLFEGKHGIFDLYFRGKYDRQAILNDLGSVYTGDRTLFKRWPTVGT
                     AHSHIHATIEIVRRHDLKIDEIREIRVFVGDYHQVMCNPLESRRAPATLVDAKFSLPY
                     IVAVAAAYRDVRLADFTAQALKNPAILAVAQRVIPAADASLDWKLSLPPGRVEVVTRD
                     GRSFKAVGTDIPGSESSPMSWDDVMAKFKDCAAASVKPLTLSQLDEVEAIARDLESVD
                     DATQLLRLLSPTANALATA"
     gene            16155..17189
                     /locus_tag="VEJ00_07785"
     CDS             16155..17189
                     /locus_tag="VEJ00_07785"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HXX01098.1"
                     /translation="MRRAVKPDLNFTPCAPRVTSQWARCCVLIAAAIACTLSFAPASA
                     QTYPTRPVKFVVPFTAGGGTDVTARLVAERMEPKLGQPIVIENRPGASAAVGAGQVAH
                     AQPDGYTILVGTATLVANTLVSGKSESFDVIDDFEFIGEIGRLDLIVMVNSKSNINTL
                     QDLVKLMRTQPNKVTFGSNGIGSPGQLGGELFKQLIKGDALHIPYKGESAALTDLLGG
                     VTTFQLCSPNVCVPRIQDGSLRGLAIAGKTRSPMAPSIPTTAEAGVPGFEAGTWYYLA
                     APKKTPASVITKLNTALNEVFADEKFRARMLETGVEVNPGTTPAGVKAAMQAEMDKWR
                     PVVKAAGIKN"
     gene            17232..19388
                     /locus_tag="VEJ00_07790"
     CDS             17232..19388
                     /locus_tag="VEJ00_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXX01099.1"
                     /translation="MSKQTPDWDPLDPKHHADPTAIHAQLRDKCPVAWSDRFGGFYAL
                     TRYSDVVNAALDPATFSNAKATIPDAAGPDRPPRAPVEVDPPVHGHFRDLLNRFFTPD
                     RIRLLEPVIRRTARDLLGRCISLGETDAVASFTFLMPIQVQCIFLGISVEDAETIKRI
                     INRIIDAGAAGDAAAHKQGNDQIYSYIEKVIEARRKTPYDANDVISALLNEPVGGRNL
                     TREDVAGVIRLFLQAGHGTTTNALGSIVRHLGTTASDQRRLREEPKLIPQAIEEMLRV
                     WTPVRLVGRKATCDVEIAGTLIPKGSRVGLMVAAANRDSTVFEDADTVNFDRKPNRHI
                     ALGYGIHRCVGAALARAQLRIAVEELLSMTDDFVVCGELEFSTWTHLGPSKLPVKFTP
                     RSVGERGTLIRAGHKELGMTVGAITPLADRIIELELRAPDGLTLPDWTPGSHIDLVLP
                     GEVVRSYSLTGDATDRQSWRIAVLHEVAGRGGSDAIHRIQSGDRVRVRLPRNNFTLKP
                     AESYHFFASGIGITPILPMIDAARAKGVPWRLDYVGRSTDKLAYLDRIAGHSQAHVHL
                     TAEVGRPDLAALLAQTTAAAEIYACGSQGFLLSLEALANEAGRSMHAEWFAPKPGARQ
                     ATEGAFEAFTVRLQRSNIEVSVLPGQSIIDACAEAGVTIPASCFEGTCGSCLSTVLEG
                     VPDHRDSFLSPSERQSNCLMATCVSKSMTERLVLDW"
     gene            complement(19472..20575)
                     /locus_tag="VEJ00_07795"
     CDS             complement(19472..20575)
                     /locus_tag="VEJ00_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXX01100.1"
                     /translation="MKITSIKAVPFTIPYHHSIVAASGTLSCAEHVLVRVNTDEGLFG
                     ISEACVRSFVYGESQASIVEAIRLWFEPALIGADPFAVEENYARIAWVVANNTARGAV
                     DIALHDLRGKITGLPSWRLLGGSGTSMRVTHILTMGGPSEAADEACEAVGQYGITSFK
                     IKVGSDIRGDVARVAAVRRAVGEDASIYVDANHGWSAEHAIRALTAMADYNVDVVEEP
                     SPAEDRIGRQRLALQVPIPIMADESAPTLGDAARELILGSARALSIKTTRTGFTESAR
                     LVALASALGARTLIGAQADSMIGAAAGLAFGSAYPRLSCEPGELDYYTVLTDQLVAEP
                     LEVTAGHLNVSQLPGIGVVIDEDKLRHYRIDRQ"
BASE COUNT         4333 a         6194 c         6176 g         4082 t
ORIGIN      
        1 gctcgagcct tcaagcttgc ggaagggcat acggcgccaa gacgggtgag gcgcacgggg
       61 atggaggtca tcgaggcgat ctaccagttc aggaccgctg agggccggtg tctgggcgta
      121 ctgcgctttc cggctgccca gccggacaag gcatggacga tgagcacctc gctgcgggag
      181 ctcaaaggat ttgaggagcc cgtgggcggc cgacgtccgg acggcaccac cgcgcgaatc
      241 ttcgggggcg agccttggtc tcggcgccgt gcccgcgagc agcgctacga cgatcgcgag
      301 ccggctgtgc tgattgtcgg cggcggccac aacggcctcg tgctcgccgc acgtctgcga
      361 atgctcggcg tcgactcgct cgtgatcgag cggatgccga aggtgggcga cgtctggcga
      421 aatcgttacg acgcgctggc acttcataac gagatcgtcc tgaatcacat gccgtacctg
      481 ccgttcccga ccagctggcc taagtacctg cccaaggacg tgctgggcga ctggctcgaa
      541 gcctacgcga tggctctgga gtgcaacgtc tggacggaga ccaacttcac cgaaggccgc
      601 tacgatgcgg ccaagggcgt atggaacgcg cgtgtacgac gcagcgatgg ctccgagcgc
      661 gtattgcacc cccggcacct ggtttttgcg aatggaatcg tcggtgagcc caagctgccg
      721 gaggtgcccg ggctgcgcga tttcaaggga accgtcgttc acgcccaggg gttcgacagc
      781 ggcgcacgct ggcgcggcaa gaacgcgctc gtgctggggg tcggcaacag cgcccacgac
      841 atcgcccagg acctgcatgg tcacggcgcg aatgtaaaaa tgattcagcg cggatcgatc
      901 tgcgttttca gcgtgaaggc cgcaagcatc aatcacgcga tttactacaa ggaagggctt
      961 tcgctcgagg atgccgacct gatcgccagc agcaatacgt ttgccgtgct gctgcggggt
     1021 tatcagctct ccacgcagcg aatgctggag atcgacaagg agcttctcgc ccggctgcat
     1081 gcgatcggct tcaagctcga tatcggccca gaaggcggcg gtcaccagat gcacgtgcgg
     1141 aagaaccatg gcggctatta tctgaacgtg ggctgctccg acctgctcgc tgatggcgag
     1201 gtcggattgc tgcactacga agacatcgac cgcttcatcc ccgaaggcgc cctgatgaag
     1261 gacggccatg tggaaaaggc cgacatcgtg gtggcggcca ccggctacca agatccgacg
     1321 gacgttgttc gttcgctact gggcgatgag atcgccgaga agatcggccc ggtgtggggt
     1381 atggaccagg gtggcgagat gtgcaacatg tacaagccca cgcctcagaa aggcctttgg
     1441 ttcatcggcg gcggcttcgc tcagagccgc gtgtggtcgc actacgtcgc gctgcaaatc
     1501 aaggcccgcg aagccggcat cgtggctgac actcccattg aaaagccgaa gctcgatacc
     1561 ctgaccggcc ctccgcgtcc atatcgggaa gccgcacttc cggcggatga tacaggagcc
     1621 ggagccggca cggacgggct gtgaccgcca agccggctcc ctacccgaaa catctggcta
     1681 ctcgcgatgc gcattcccta ctggagccct gagctcaaac ggcagcccga tgacttggtg
     1741 aaggcgatcc tcgatcgccg gggcggatcg cttatcaatc tggaccgcgc gctgctctgg
     1801 agcgagccgc tggctcgggg atggaacacg tacctgaaag ctgtgcggtc ggagcttggg
     1861 accagccgca ggttacgcga attggggatc tgcgccgtcg cactgatcac gggcgcggat
     1921 tacgaatacg agcaccatgc gccggactat ctggccgccg gagggtccca ggccagtctg
     1981 gacgcgctgg ctgcctacgt gaaatcgggt gttaaccgtg atccgcacgg attgtccggg
     2041 gacgaagcgc ttgtcgctca gtatgcagcg caaatgactc gcaatgtgca agtcgacgac
     2101 gggctattcg acagaatgaa ggatcgattc gataccacac agttggttga gatcactgcc
     2161 gcgattgcca catacaacat ggtggctcgc tttcttgtag cgctcgacgt cactcctgaa
     2221 gcctagacgg ttgccgaacc ggtgatcgta gggactccga tgcatcgcct gaaagataag
     2281 gtggccatca tcatcggggc cggccagagt cccggcgagg caatcggcaa tggacgtgcg
     2341 accacgatcc ggttcgcgca ggaaggcgcc tccgtgcttg ccgtcgaccg caatctggcg
     2401 tcggcgcgag aatccctcga gatggccggt actgcggtgg atggcgaggc gttcgaagtc
     2461 gacgtcgcaa gatctgaaat gctgcaggcc gcggttgacg ccgcgatggc ccgctggggt
     2521 cgcatcgaca ttctccacta caacgtgggc gtgagtgtga tgggcggcga gcagaccctc
     2581 gacaagttga ccgacgaagt gttcgacctg atcaatgcga tcaacttgcg tggcgcagtg
     2641 atggctgcaa agttcgtcca gccgatcatg agaaagcagg ggtcgggcgt gttgatcaat
     2701 gtcgcctcga tcagtgccat tgagaccttc acccatctcg tcgcctaccg aacgagcaag
     2761 gccgggatgg ttgcgttcac gaggcaattc gcgatcgaga acgcggaata cgggatccga
     2821 gccaatacca tcctcccagg gctcatggaa actgcaatgg cggtcgatac acggatgcgg
     2881 ctcagcggcc ggtcgcggga agacatcgtg gcggagcgaa acagcaagat cccgctgcgc
     2941 aggggcggca gtgggtggga tgtcgccaac gccgctctgt acctggcgtc ggatgaggcg
     3001 tccttcgtca ccggaatcga cctgccggtc gacggcggta cactggccag gatcggctgg
     3061 taggcccggg cgaatctcgg cgccgatcag cggtaagtag acgagcaatt gggatggatg
     3121 ttatgcatga gctcgccgag atccgccaag cgctcgaggc tccagatcat agccgttgca
     3181 gtctcgatgc catccgcaga caggactccg gtcgcattgc ggcgaaactt ttcgaagagc
     3241 tcctcgttgc ttacggtcgt tgacgggtcc ggcgagcggc cgccctttgg atatcgtttc
     3301 tcgccagtgt aggtctcacc acgcgcacgc acctcgaccc gcgcaggccg acttgcggga
     3361 tcactcgtga gcaatttccc gtaatcggga tgtgcttcga cctcgatctt gtccatgaga
     3421 gccaacacgg acgggtcgaa gacgacttca ggcgactgcc aggccttgtc cggcggtatg
     3481 aggtgcgctg ccactgagag accatgcgcg atgctgaact gtgcctgcgt gacatgctcg
     3541 atgtttcgat tcagccacag cggctgtatc acgtgagcct cgacccaggc cttgatgttc
     3601 tcgatctccg aaggcctgat acggttgctt tgaacgatgt ctctgacgac gtcgatcggc
     3661 ccatgcaggg cgccgcagtg cggataagac ttgtaggtga ccttggccgg gaaaagccac
     3721 acctcgccaa ggccgggtgt taccgaagcg gggtcccacc gcgagctgct gatcatcttc
     3781 ggccagccac tcacgggatc atccagcagt tgccggtctc ccgtatgccc caactgggcc
     3841 agtgtggcgc ccgtcattgc cgtttggacg agcgcgccgg cgaccgcgta tttaaccgtc
     3901 gcggtgggca aatgaaccga ccaggaccat tgtgcattca cgggtgacat gctcgccgca
     3961 ataccgatgg cgtcggtcgt catttgcggc gaaaaccgcc tgactcgccc aaccgctgcg
     4021 gcggcgccga aaatggtgct cgaatagccg aatacgggag gtggagagac cttcccgtcc
     4081 ttgatgtcgc gcagatagtc cagccccttg cccatgcgat gcgacaattc atgcgctacc
     4141 gcaatgccgc acaagaggtc cttgcccgac gcgccactcg cctctgcagc cgccaacgcc
     4201 cccggtatga cgtagggact gacatgaccg ggcggcagca gagcatcgaa gtccaaagca
     4261 ttgatcaatt cggcgttggc gaagccggct gccacgcaag caaccttgtc gccggtccca
     4321 atgatcgacg cctggtcagc gggcgccccg ggaccctgcc gacgcgcgta ttccacaccg
     4381 atggcgccct tcgggtggct caagcccccg agcgcacagc cgaccgcatc gagaagcagc
     4441 cttttgcttt cgataaccac atcggaaggc agggtatcga agtcggcgct ggcggcaaaa
     4501 ccagcaaatt gctctacgag cgtcatgtct tgtcccatgc attgaagctt gcggtcgatc
     4561 ccctcgctct ccaagtctgg accatgggcc ggattctatc cgcgggtgga ataccgagca
     4621 ggcgtcgcag tggcaatatg ctttgcgctt gtggcaaagc tgcttccgga ccagcgcgtg
     4681 ctgagaattg gatacttgca ctgttcttgc cgccgagtgc aaagctgctt gccgggacaa
     4741 cgcgttgctg gagcgcagcc ggccgtgaat actgcggcac gtacaattcc gtcaaaactc
     4801 cgcgagtcgg acgctcggcg tacaaccaca ggacatatcg attgccatga atattctgaa
     4861 agccatgctg cggatgcttt tcgtcttcgt cgcgtttgca gcttcgccgg tcaccgcatg
     4921 ggcgcaacaa tacccgaacc accccatcaa gctgatcgtc cccttcgcac ccggaggtaa
     4981 taccgatgtg gccggccgcc tgatcgccga tggtattggt caaggtctcg gtcagacgat
     5041 catcgtggaa aacaagcccg gcgcaggcgc cagtatcggc gcggagctgg cggcgaaggc
     5101 tccgccagat ggatacacgc tgctcttggc gtcggacgcg cagaacatca atccagcgat
     5161 ctacacgacg ctgcaccacg acatcatcaa agattttgag ccggtcggac tcgccatggt
     5221 gacgacgctg gtgctggtcg cctcaaacag ttcgggcatt cactcagtcg ccgatctcat
     5281 cacggaggcg aaagcaaagc cgggaaaggt gacctatagc tcagcgggag ttggaagcgg
     5341 ctctcacatg gctggagcat tgttcgacca ggctgcgaat atcaagacgc tgcacgtgcc
     5401 ctacaaagga tccggtcctg cgacgaccgc agttgtcgcc ggtgaagtcg acatcaccta
     5461 cacgagccta gcagccaccg tcccgttcta caaggagaag aaactgcagg ctcttgcgat
     5521 tacgggcaaa gatccgagcc ccctcttccc gggcgtcccg accaccgagt ccctggggtt
     5581 caagggatat gaagccgggg accacatcgg tctactcgct cccaagggga cgccgaagaa
     5641 tgtcatcgat cgactcaaca ccgagttgaa taagtggctg agcaaacctg agaccaaagc
     5701 tcggcttgaa cagttgggct ttactccacg atcctctacg cctgccgagt acggtgcatt
     5761 cattcgttcc gaggtcgcaa agtgggacaa aactgccaag accggcaaca tcaaggtgga
     5821 gtgactagcc accactagtt gcccgcggta aacccacaaa tcaaacggcc cgcaccgttg
     5881 cgggccgttg ctttattcgt cgcgggcact cgtgcaatcg actacccgca gtggtcaaag
     5941 gctgccggct agtcccgcct cgcgcactgc ctgtccccac ctctcgtatt cgctgcgttc
     6001 ataagccgcg aactgctcgc cgtgcagatt cataacttcg ttgcctgtac tggcgataaa
     6061 gtccttcacg gctttgctgc tctccgcctt ctttagcgca tccagcaaca ctgccacgac
     6121 tgcgggaggc gtacccgctg cgacataggc ggactgatac ccgacgattt cgagcggaac
     6181 gcccgcctcc gtcatggttg gaacatcggg aaagcttaac gatctggtct tcgcacagac
     6241 tgccaggcca cgcatccctt tcacttgata aggcagcaag gccgcaccat cggcgaagaa
     6301 gaagttgatc cggttcgtca gtgtgtccga gatcatgtcc ccgaaattct tatatggcac
     6361 attcagcagc ttgatgccgg ttgcttgcgc aaacattcca ccggcgaccc gagttgtcgc
     6421 agagacactg ccgtaggagt acttttcggg atctcgcttc gccgcggcga caacttcctt
     6481 gactgtcttc aagggaaact cgggcccggc gatcatgacg agcggagtct tcatcgtccc
     6541 gcccaacgga atgaaatcct tcaatgggtc atagggcagc tgcttgaaga ggtggggatt
     6601 gacgacctgc gaagagattg acgagaagag gatcgtatat ccgtcggccg gcgcggattt
     6661 gaccgcctgg atgccgatca ggaattcacc gcccgggcgg ttttcgatga tgaaggtcac
     6721 gcccaggatc tcgcccattt gctgtgcata gatccgggcc agcgcatcga ccgtgctgcc
     6781 tgccgccaag gggacgatgc ccctcatagg ttttgacttg tcagggaagg tagcctgggc
     6841 gaaggccgcc ttccccaagc cgaaagcggc acctattgcg gcagtgttag cgagaaagct
     6901 gcggcgtgag cgaagtcgag ccattgccat gtctccctta gatgcaatcc gattggacct
     6961 tcgttcgcgg cgctcagaat acgaacgccc ctttgccagt ggactgggta tcgaaaagtc
     7021 gataggcctc ttcggcctgt ccaagtcgcc agcgatgcgt gaacagccga tcgatgtcga
     7081 gcgcccgatg cactacgaaa tcggcacact cctgctgact caccgtggac atcgtgtagg
     7141 acgtcatgac cgtaatctga cggtcgagca atgcctgcat aattagattc acgctcgcgc
     7201 cgatgccaac catgcacatc cttccccaga ccgccaacgc ggccagcccc gcactggccg
     7261 cgtcagatga gccagatgtc tccattacgc actccacccc gcggccgtga gtcagatcgc
     7321 gcaacgcttg cacgggatcg gcctccagag gattcacgag gttgtgcgcc ccaaacgatg
     7381 cggcgatcgc aaggcgatca cggtcgatat cgatcgcgac gactcgtgct ccttgtgctg
     7441 ccgccagcat cgtcgtcgaa agtcccaccg ggccttggcc gaacacggca agcgtttcat
     7501 cgccgcgaac ctggagccgc tcgagcgcgc cccaggcggt tcccgtgcca caggcgatgg
     7561 cggcgcccgc ctcgaagctc agcgcttcgt gcagcgggac cagagtactc gctgcgacct
     7621 tcatatacgg cgcatgtgcg ccgtgcgcgt tgccgctgta cgtcgtgcgc attggcggcc
     7681 gacacagttg tggccaaccg gtgcggcaat gaccgcagac accacagccg tggtagtgat
     7741 gcaccatcac acggtcgccc ggcttagcca cgtggcggtc cagaccaggc ccggtggcga
     7801 aaacaacccc ggccggttca tgaccgccaa taaagcggcc ggtcaacgga atgtcggggt
     7861 tgtttggagc gcggtagggg tgcaggtcgc taccgcacat gcccgatgcc ttaatctcaa
     7921 cgacgacctc gcccgagccg ggcgtggggt cgtcgacttc aacgaatgcc aagcgacgat
     7981 cacccaaaaa tacgagtccc ttcatcgatc gtcctgagcg gtgcgaggat ttcgtagccg
     8041 aaacacggga aagaaagctt ttcatgtacg ggaaagcaac gtccagtcgt tttttttgac
     8101 ggtacggtaa ctgcatcggc agcacacccc atagcttgca tgctgctgtc tgcagttcac
     8161 cgttttgatg ccgcctcgcc cggcctgacc gttcccctta ggggccgaac acggctttag
     8221 tgggacttac cgtgaaataa agaaacccaa ctggacggga cgcgcacgct caagggacac
     8281 tcttttcagg tctggccgcc ttcaaagagc aaaaagcccc gggtgccccg gtctggttag
     8341 caccactatg ccgattggac gccgccaaat cggccgcgca aagtctgtgt tcggcaacct
     8401 gcgcacgacg tgtccttgcg cagaaaccac atgcatgatg aagaaatgga tggctgacat
     8461 gagggtagac cgctctggca acgtgcctca aattgcacgc cgcgcagctc gatacgccgg
     8521 actgtacagc ctcgccgcga cagcggctct gttcgtccta atggcgtcga acgccatgag
     8581 tcaggagccg acgttgcctc cgcttattcg aatcgtcgtg ccattcccgc ccggtggtag
     8641 taacgacatc gccgcgcgcc aggtcgccaa gcagcttgct tcccggacag gaaaaaacgt
     8701 catcgtagag aatcgcgcgg gcgcatccgg attcatcgga gcaagcgtgg ttgcacggtc
     8761 acccaaggat gggtcggtac tgcttctaac gtcgtctagc acggtaagcg ctgccgcaac
     8821 caagaacaac gttaccgttg atttcttaaa ggacctgatg cccgtatcga tactttcgga
     8881 atcgccgctg atgattgtgg tctccagcga atcaaagatt cagacacctg gcgatctgat
     8941 cgctgctgcg cgcgctaagc ctgatacgtt gacgaacgga acgcctggta acggcacaat
     9001 tggacatcta actagtgagt tgctcaacga cgctgcaggg atacagataa aacacattcc
     9061 ttacacaggt ggtggacctg ctatggtcga cctcctcgca aatcgcatag acatcaacat
     9121 cgtctctaac agcgcagccg ccggttacgt cacccaagga aagcttcgct cggtcgcgat
     9181 tacggccgcc aagccaagct ctgattttcc cggtgttcca accgtcgcgt cggttgtgcc
     9241 tggttttgaa gtcgtccagt ggcaaggcgt gtgggccccg gctggcacac ccgacgcgat
     9301 tgtcgatcgc ctcaaccggg agctgaacca gatcgtaaag acaccggaat ttgcggtatt
     9361 gctgcacgaa gacggcggca ttccgcaatc actcacaccg gcgcaggcgg cgattaaagt
     9421 ccgcgacagc tatgagatgt ggagaaaact cgtcaaggcg cgcaatctca tcgtcgattg
     9481 attgcgttct ttttgcttgc taggccagga ggcgctatcc ggtagactct gcagttcctc
     9541 gcaaggccag cctgaagtgc gagcgtttag ccgaggcagt caaccccaca gcgcggtggg
     9601 aagcggcagc ccatcgtaaa gcgcggctgg aatgagcagc gcccgatttt cgccagcggc
     9661 gagatgatcc agctggcgca agtgctgagc cgcatgccat acatagcgtt ccagcacgat
     9721 gtcggcgggc tggttgccgt aatgggtgtt caggcgcgtg ggaatggacg aaggaccacc
     9781 gctattaatc cattgatcgt agttagcgca catgcggtcg gcatacgata aaagagccgc
     9841 cctgctccga atcgacctgt ctggcgtaag gtagattgcc cgcgcgtcga tcgtgtcgtc
     9901 ttcgatcagg cgaaggaagg actcgacaat ctggtaggcg tgttccgcaa gttcgagaac
     9961 gttcgcgtgc ggaccgacca gcttgtgacc ttccagttct tcctcgggga aggtgtcaat
    10021 aacttcccgc cccttgtcct gaagtgcgtc ccagcgggtg acgagcacac tccgcggcaa
    10081 ccgccgctcg acacgggtca cgccgaagaa cgcagccaga tcatctagcg actgcgcgta
    10141 cacgatcttg tcacccttgc gaaccgccgg cgcgccacgt atacccgcgg ccgccacttc
    10201 ggctagcgca gcagggtgct cgaggatgtt caacgactcg aaaggctccc ccaggctggc
    10261 gacgaattcc ttcgccttaa tgcagctgga acaacctggc acccaataga ccgagatatc
    10321 gggtctaggc acctgcgctt cactggtcag tgacatgtga tattgaccgc tttccccggt
    10381 gagagcttct ccaggatcgt atccatcgag gaccaatttt aactatggtg gaccaccgcg
    10441 tcagacgttt tttcttagcc tagcgtaagc aggacttcca ttgtttcggt gaccggtgca
    10501 gtacccaaga tgaaagagcc gtggcgcgcg aaacaacgtt agttatcctc aatttctgtg
    10561 gatatccatg tggacccaat ggcttgtcgc tagccactgt gcggccgcgc gtgatttgct
    10621 cgaaatttgt gcgcgaccca cattgacaaa tgcgcatgcc gatcatgtcc ttcgacgcgt
    10681 ccgagcctcg gtcaacattc caactcatca gtgccttgat cgcaggtcag gtgctcccgg
    10741 taatcatcga ccatcgccga cgtcgcccac tccagaaggg cgtcagtggg ccttaccgga
    10801 ggcaaagaaa atccgtctgt acacccggtc tctgctagcg gcagacttcg ctccgaacga
    10861 gcggaacccg cacctcgccg atggagcgtc gcaccagcga gagcggaatg ccgcaagtct
    10921 ttggtgtact cacatcagcg cctatagggg gaccatatga agcataccgc tttgctgcga
    10981 ttaatcgctt tcatcagttt gtcgctgttt acgatcgctt ccgcggtcgc gcaggagtat
    11041 ccgactcgac ccatcaagtt agtagttggg tacggcgcag ggggaacagc tgatgcgacg
    11101 gcgaggatct atgctgaaaa gcttcagacc atcctcaagg ttccggtgat cgttgacaac
    11161 aagcccggcg cttatgaaca gatagcggcc caagcggttc tctcagcccc gcccgacggc
    11221 tacacgctct ggtggggtac gaccggcgcg ctgacgatgg gccccggcat tcggacgaac
    11281 acgccgtacg acgtaattaa gaacttcact cagataggaa aaatagcaga ggttgaggca
    11341 gttctcgccg tcaagaagga ttctccaatc aactcgctgc cggacctcat caaatttgcg
    11401 aaggccaacc ctggaaaatt gttctacgct tctgcgggag tcggatcagg gaaccatctt
    11461 ttgaccgaat acataatgaa tcaaacgggc atcaagatgg agcacgtacc gtacaagagc
    11521 gatgccgacg tggcgcgcga gctggccgcg ggcacggcgg atttcggcat tcccgtcgcc
    11581 ggcgtcgcgg tcccttttgt caaggaaggt aagttcaagg ccatcgcagt gactggaatg
    11641 caacgcctga agcagcttcc caatgtgcca acgatgggcg aggaagtgcc tgcgctgaac
    11701 attggcgttt atgcgattta tggattgcta gcgccgttgg ggacgcctgc tccggttgtt
    11761 cggacattga gcgatgcctt caacaaggtc gcggaaatgc ccgatgtggt gagccgtctt
    11821 gaggctacca atgtgaggcc ggcgacgtcc aatcccaatg acttccgtca atacctcgag
    11881 gccgaaggaa agaggtggcg cgagctcggc aaatcgctaa acatcacctt gaattgatag
    11941 acgcgtctgg cgcccgggcg tgtttggctg cttcgagggc taggcatgct cggcgctgac
    12001 caggtgacca ttctgcaaag aatgcgtcta ttcccctgac aaggttttgc gatcctgcaa
    12061 tcaaaactcg cgtgtcttca gattctaact tgtgggacga cgtcgtcatt ggcgccggaa
    12121 gcgcgggctg tatcgtggcc gctcgcctca gcgaaaacaa cgcgcggcgc gttctcctgc
    12181 tcgaggccgg cccgcgcgac gtttggctga tgaagccatt cggcctcagc tactacctcg
    12241 acttttcgcg atttgagtgg ggattctggt ctgagccgga cccgacgcgg aacatgcgct
    12301 ccgaccattg gcggcgcgga cgcgtcgtgg ggggcagcgg cagcatcaac gggatgaact
    12361 acgtgcgtgg cactcgcgcc gactacgacc ggtgggctgc aatgggcaat gacggctggt
    12421 cggctaacga cgtcatgccc cttttccggg acatggagca ttgcgagccc ggctaccaca
    12481 ccccacccga ctactcgatc cgtggcgatt ccgggccgtt ggccatccgc gaggtccgaa
    12541 actgtcaccc tctcaccgaa gcgttcctca aagcggcgca ggcagcaggc attccgcgaa
    12601 caaacgactt caatggcgcc cagcaggaag gggtgggcta cggacaattc aatcagcgcc
    12661 gggggatgcg tgtcagttcg gtcgatgcgt ggctgaaccc cgcgttgcgg cgcaataacc
    12721 tgaagttgtt gacggacgcg ctcgtgcaca ggctgcacgt gaaggacggc cgggtcaccg
    12781 gggtcgccta cgaacgcgac ggtgcaatgc gcgaagcgcg agctggccgc gtcatcatat
    12841 gcggaggtgc catcaatacg ccgcagctgc tgatgctttc cggaattggt gacggcaagg
    12901 agctgcaatc gcttggtatt gatgtcgtgt tgcaccgtcc caccgtggga cgcaacttga
    12961 tggaacatcc cctcataagg ccgagctggc gggtcaacaa gcctagctac agtcccacga
    13021 acaacatgct acggatggcg ggctttggcg ccaagttcct gttcacgggt caaggaccgc
    13081 tcgccacgat caccgaagcg atggggttcc ttagaacatc accgcaggaa cctgcgccgg
    13141 acgtgcacat gctcttttcg gtcgcaggcg cggttaacga taacgagctc aagttttaca
    13201 agagtctgaa ggtcctgccg tatccgtcgt tctcgatggt gctcgacaag agctacccgc
    13261 ttagccgcgg cacgatccgt cttgccagcg cggaccccaa agcggcgccg ctgatccacc
    13321 ccaatcttct gggagatccg cgtgatcttc aaaccctggt gagggccgtc ggtgtggtgc
    13381 gccgaatcgt ctccaagtcg ccattggccg acatggttgt cgacgaagta atgccaggta
    13441 aacacgccga gtccgagcaa gcgctaatcg agtacgtcaa ggatcagacc gggctcgcct
    13501 accatgcttc aggcacctgc cgtatgggcg tcgacgaagg cgcggttgtg acgcccgacc
    13561 tcaaggtgag aggcgtggaa aatctctgga tcgccgatgc atcagtcatg ccggatctga
    13621 taagcggaaa tatcaacgca gcctgcatga tgattggcga gaagctggga cgacagctca
    13681 gacactgatg cgacggtcct agccgcactc cggagtccgg ccggaagcgc cggttacgac
    13741 cgagaggccc accgttaagc cagcggtgca ggttactgcg ccccgccccg cctaccccaa
    13801 atcagactat cgcaacagat ctcacgaaga gatcggcttg taatcgaaaa tgaacttccg
    13861 ccaccggctt acggtgtaag tgctaaagat gccgttgagg taaaaagggt cgttttcgat
    13921 gtagtcgatc gcgtcctgcc tcatatcggt gtccaggatg atcagccccc cgttggggaa
    13981 atctgcgtca tcgggaaaca gaccaccgct cgtaatgatg cgatgcgcat tcttcaacac
    14041 atactccaag tgcacgggtc ggactcgctt tttcttctcg atgactccgg gatcgtcaac
    14101 aaacatgatg acatagggca tgttgtacct cttccatttg gtgtttgcgc actttgcccg
    14161 gtgagcaggc gcacgtccat aaggattttc taggttcaaa tgtaagctgg gctaaccctc
    14221 tgccgggttc gcacgacaca tcgaatgcca ctccatcgcc agctgacctt cagagcgaaa
    14281 agtgccgatt cagcagcact tacgcttaac taagaaaata cgtctatacg cacgtgcgcc
    14341 ctcgcgggca cactcccatc ggggaactga tagcggccac gccgtttagg gttgagccgg
    14401 tgttatacgt tgagttgcac gttgccagct tggcaccgtc atgtaatgca ggacgagagc
    14461 caatagtttc tggaggaaaa aagtgtcgac gtctcccgac ctgtgcttcc actttgcgaa
    14521 tcacgccgtt gcgacgacct atgatcgctt gccaccaagc gcgatagata tcgccaaaaa
    14581 gagcgtcgtc gattcacttg gggttatcct ggctgccagc gggatggagc ccgctgtccg
    14641 gggcgtcatt gacctggtga aggaaagcgg cggccgcccc gaagcctcga tcctggcctt
    14701 cggagggcgt gtgcccgcaa tgatggcggc gctggccaac ggcgcaatgg cgcactgcct
    14761 cgattacgac gaccaaactc cctggggaca gcattcggga agctccctgc tgccggctgt
    14821 atttgccgct gcagaaaaaa agggtggtgt ctcggggaag aagatgattg cggcagtggc
    14881 tgttgggcag gacatcttta atcggctgcg atgccatgtc gagtgggaga aggattgggt
    14941 attcaccacc gttatgggcg tctactcagc aaccgcggcg gcgggtgcgg tgcttgggct
    15001 gcccagagat cgaatagcga acgcccttgg gatcgctagt atgcaatgct ccgggacgat
    15061 gaatatgatc cacgcggtag gcagcgatct acgtgcgatg tatgccggct ttccggcgaa
    15121 gggagcggtc ttgtccgctt tgctttccga gaaaggtgtc accggcgttc ccgatctctt
    15181 cgagggaaaa cacgggatct ttgacctgta cttccgcggc aagtacgacc gtcaggcgat
    15241 tctgaatgat ctcggcagtg tctacacagg cgacagaacc ctcttcaagc gctggccgac
    15301 tgtgggaacc gcacatagcc acattcatgc aacgatcgaa atcgtgcgaa ggcacgacct
    15361 gaagatcgac gagatccgag agatacgggt cttcgttggc gactatcacc aggtcatgtg
    15421 caacccactg gaatcgcgga gagccccggc gacccttgtg gatgccaagt tcagcctgcc
    15481 gtacatcgtt gcagtggcag cggcttaccg tgatgtgcgg ctagctgact tcaccgctca
    15541 ggctctcaaa aatccggcaa ttctggccgt cgctcaaagg gtaatccctg cagccgatgc
    15601 gtcgctcgac tggaagctca gtctgccacc gggccgcgtt gaagtcgtta cacgcgatgg
    15661 tcggtcattc aaggcggtcg gaaccgatat accgggtagc gaatcgtcgc ctatgagctg
    15721 ggatgacgtc atggcgaagt tcaaagactg cgccgcggct tcagtcaaac cactgaccct
    15781 ttcgcaactg gatgaagtgg aagcgatagc acgtgatcta gagtcggtcg acgacgcgac
    15841 ccaattgttg cgcctgcttt ctccgacggc taacgccctg gctactgctt gacgatggcg
    15901 tccggcacgt ttgctccttg cgcgccagct caaggaattc acagccctcg tctgatcaag
    15961 tcagcttagt ccgcgcgttc ccttgctctc cgaccgcgaa gtactcgata tcctgcaaat
    16021 cgctgacagc gcgcggggat gagaaaccgg cattcagctg gccttactac acgttaaaaa
    16081 gaaacgtctg tacctgcgta tagagcgccc gcagactgta tcagattaga agaccgtaat
    16141 tcaggaggtt agatatgcga cgagccgtca aaccggatct taacttcaca ccctgcgcac
    16201 cacgcgtcac gagtcaatgg gcaaggtgct gcgtgctgat cgcggcggcc atcgcgtgca
    16261 cgctttcctt tgcgccggcc agcgcacaaa cctatccaac gcgaccggtc aagtttgtcg
    16321 tcccattcac ggcgggcggc gggaccgatg tgaccgcgcg gcttgtagcc gagcgaatgg
    16381 aaccgaagct gggccaaccg atcgtgatag aaaatcgccc aggcgcaagt gccgcggtcg
    16441 gcgctgggca ggtcgcgcat gcacaaccgg atgggtacac catcctcgtc ggcaccgcga
    16501 ctctcgtagc gaatacgctc gtgtccggta agagtgaaag cttcgatgta atcgatgact
    16561 tcgaattcat cggagagatc ggacggcttg atctcatcgt catggtcaat tcgaagtcaa
    16621 atatcaatac actgcaggac ttggtgaagc tgatgcgcac ccagccgaac aaggtgacgt
    16681 ttggctccaa cggtatcggc agccccggtc agttgggtgg tgagctgttc aagcaattga
    16741 ttaagggtga cgcgttgcac attccctaca agggtgagag cgccgcgttg accgatttgc
    16801 tcggaggtgt cacgacgttc cagttgtgct ctccaaatgt gtgtgtgcca cggatccagg
    16861 atggctcgct gagaggcctg gcaattgcgg ggaagacccg gtctccgatg gcgccctcta
    16921 ttcccacgac cgcggaggcg ggtgtgcctg gcttcgaggc gggaacctgg tactacctcg
    16981 ccgcgcccaa gaagacgcca gcgtcggtga taaccaagct gaacaccgcc ctgaatgagg
    17041 tgttcgccga cgaaaaattc agggcccgga tgctggaaac gggcgtggag gtcaaccccg
    17101 gcaccacgcc agcgggcgtg aaggcggcca tgcaggctga aatggataaa tggcgccccg
    17161 tcgtgaaagc cgctgggatc aagaactgat cgtatccacc agactggttt cgcaggaaga
    17221 cgacgaacga gatgtctaag cagacccctg attgggatcc tcttgaccca aagcatcacg
    17281 cggatccgac cgcgattcac gcgcagctgc gtgataaatg cccggtcgcc tggagcgacc
    17341 ggttcggcgg cttctacgct ctgactaggt attcggatgt cgtaaacgct gccctagacc
    17401 ccgcaacatt ctcaaacgcc aaggcgacga ttccggatgc cgcgggtccg gatcggccac
    17461 ctcgcgcccc agtggaggta gaccctccgg ttcacgggca tttccgcgac ctgctcaaca
    17521 ggttctttac cccggatcgc atccggctgc tcgaacctgt aatccgtcgg acggcgcgcg
    17581 atctgcttgg ccgctgcatt tctctcggcg aaacggacgc cgtcgcttcc tttacgtttc
    17641 tgatgccgat tcaggtgcaa tgcatctttc tcgggatctc cgtcgaggac gcggaaacca
    17701 tcaagcggat cataaatagg atcatcgatg ccggtgcggc cggagatgcg gctgctcata
    17761 aacagggcaa cgaccagatc tactcgtaca tcgaaaaggt gatcgaagcc cggcgcaaga
    17821 caccctacga tgccaacgac gtgattagcg cgctgctgaa cgaaccggtg ggtggccgta
    17881 acctcacccg cgaagacgtc gccggcgtca ttagactatt tctgcaggcg ggccacggca
    17941 cgacaacgaa tgcgttgggc agcattgttc gtcaccttgg caccactgca tcagaccaaa
    18001 gacgcctcag agaagagccc aagctgatcc cgcaggcgat cgaggagatg ctgcgggtct
    18061 ggacccccgt acggttggtg ggacgcaagg caacctgcga tgtcgagatc gcgggcaccc
    18121 tgattcccaa ggggtcgcga gtcggcctaa tggtggcagc agccaaccgt gattcgacgg
    18181 tattcgaaga tgcggacacg gtgaacttcg atcgcaagcc gaatcgacac attgcgttgg
    18241 gctacggcat ccaccgatgc gtgggagctg ccctcgcgcg cgcccagctg cgtatcgcgg
    18301 ttgaagagtt gctgtcgatg acagacgatt tcgtagtctg cggcgagctt gagttcagca
    18361 cgtggacaca ccttgggccc tcgaagctcc cggtgaaatt cactcctcgc tcggtcggcg
    18421 agaggggaac cctgatccgg gccgggcaca aggagctcgg catgacggtc ggcgccatca
    18481 cgcccttggc cgatcgcatc atcgagttag agctgcgtgc tcccgacggg ctaacgctgc
    18541 ccgactggac gccagggtcg cacattgatc tcgttctccc gggtgaggtc gttcgttctt
    18601 actcattgac cggggatgcg acggatcgcc aatcgtggcg catagccgtc ctgcatgaag
    18661 tggctgggcg cggcgggtcc gatgccattc atcgcatcca gtccggagat cgcgtccgag
    18721 ttcgtttgcc tcgaaacaat ttcaccttga agccagctga gtcctaccat ttcttcgcca
    18781 gcggcatcgg aatcacccca atcctgccga tgatcgacgc agcccgcgcg aaaggtgtgc
    18841 cgtggcggct ggattacgtg ggacgcagta ccgacaaact tgcgtacctc gatcgcattg
    18901 ccggccacag ccaagcacat gtccatttga ccgctgaagt aggacgcccc gatcttgctg
    18961 cactgctcgc acagactacg gcggcggccg agatatatgc ctgcggctca caagggttcc
    19021 tgctaagcct cgaagcgttg gccaacgaag ccgggcggtc tatgcacgcc gaatggtttg
    19081 cgcccaagcc cggcgcgcgc caagccaccg agggagcatt cgaggcgttt accgtgcgcc
    19141 ttcagcgctc gaacatcgaa gtctcggtat tgcctggtca atccatcatc gatgcatgcg
    19201 ccgaggctgg cgtcacaatt cccgcctcct gcttcgaagg cacctgcggg tcctgccttt
    19261 ctaccgtgct cgagggcgtg cccgatcacc gagattcgtt cctctcaccg agcgaacgcc
    19321 agtccaactg tctaatggct acttgtgtat ccaagtcgat gacagaaaga ctcgttttgg
    19381 actggtagga gtgccgagcc gcaaggtgcg ctcgggtttt cgacctttga ccagcatttg
    19441 accgtgcacc tgtctttgct gctgtgactg ttcattgcct gtcaatccgg tagtggcgaa
    19501 gcttgtcttc atcaatcaca acaccgatcc ctggtagctg ggatacgttc aggtggcccg
    19561 cggtaacttc aagcggttcg gcaacaagct ggtcggtgag caccgtgtaa tagtcgagct
    19621 ccccaggctc acaggaaagg cgtgggtatg ccgagccaaa ggcaagaccc gcagccgccc
    19681 caatcatgct gtccgcctga gctccgatga gtgtgcgtgc ccccaacgcg cttgccagag
    19741 ccacgagtcg cgcggactcg gtgaaacccg tgcgtgtggt ctttatgctc aatgcgcgtg
    19801 cggagcccaa aatgagctcg cgagcggcgt ctcccagcgt tggagcactc tcgtcggcca
    19861 taattggaat tgggacctgc agtgccagac gttgacggcc aatccgatcc tccgcggggc
    19921 tgggttcttc cacaacgtca acgttgtagt cggccatagc cgtcagtgct cggatagcat
    19981 gctctgcgct ccagccgtgg ttggcgtcga catagatgct ggcgtcctcc cctaccgccc
    20041 tgcgcaccgc agccacacgc gccacgtcgc cgcgaatatc gctaccaacc ttgatcttga
    20101 atgaagtgat gccgtattgg cccacggcct cgcaagcctc gtcagctgcc tcactgggcc
    20161 cgcccatggt caaaatgtgg gtgacgcgca tcgaagtacc actgccacca agcagacgcc
    20221 acgacggcag gccggtaatc ttcccgcgga gatcatgcaa tgcgatgtct acggcccccc
    20281 gagccgtatt gtttgcgacc acccaggcaa tgcgcgcata gttttcttcg actgcgaaag
    20341 gatccgcccc aatcagcgca ggctcaaacc atagtcggat cgcctcgacg atcgaggctt
    20401 gggactcccc gtatacgaac gaccggacgc atgcctcgga aatcccgaac agcccctcgt
    20461 ccgtgtttac gcgaacgaga acgtgttctg cacacgaaag cgtgccagaa gcagccacga
    20521 tcgaatggtg ataggggatg gtgaagggca cagccttgat tgacgtgatc ttcattcgtc
    20581 tactccgtat ttctcttgga ttggcttcgg accagaactg gcaatgtgaa cgaccctcta
    20641 aagaccacga gtgtttcgca gtgcaaggcg gtagtccagt cgccttttta ccgtcccacg
    20701 gtaaggtcga gtgaccgtaa gcgaaagtcg attgtttgaa aagaaatggt gactttacgc
    20761 gctcaccgat ttccattgga atcgc
//
LOCUS       DASPUL010000316         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_200631, whole genome shotgun sequence.
ACCESSION   DASPUL010000316 DASPUL010000000
VERSION     DASPUL010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200631"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VEJ00_07800"
     CDS             complement(<1..841)
                     /locus_tag="VEJ00_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXX01101.1"
                     /translation="MQSLPSSLAWAHPLVAEWFVRRFGTPTEPQEQGWPHILAGRTTL
                     ISAPTGSGKTLAAFLACIDRLVRKALAGDLADRTEVLYVSPLKALGNDIQKNLEVPLG
                     EILALAGERGLLMPEIRTAVRTGDTLQPERRAMLKRPPHILVTTPESLYILLTADRSR
                     AILRDVGTVIVDEIHAVADDKRGAHLALSLERLDALAHARPVRIGLSATQKPIEEVAH
                     FLTGSGRADPVIVNIGHKRKLDLAVEVPPMPLGPIATNEMWDTIYDRLVALVGEHRST
                     LVFV"
     gene            1029..1913
                     /locus_tag="VEJ00_07805"
     CDS             1029..1913
                     /locus_tag="VEJ00_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXX01102.1"
                     /translation="MRKILWATAALLPMLASPLLFAQGPHHFEPGFNLFSKEQDVQLG
                     EEAAAQFRKQMTVVNDPDLTAYVNSVGKRLAASREAQGSGFPFTFEVVADPTINAFAL
                     PGGHMFIHTGLLKAVDNEAQLAGVMGHEMSHVILRHGTSQASKANLIELPLMLAQQIT
                     NKDSMMGKLEQLGIGLGATGLLLRFSRGAESQADLTGSHLMAESGYDPIEMAHFFDKL
                     SGPGKGLVGNVEQFMSDHPNPGNREQAIEDEARLLPAQKYGYETGDFKRMKSIVKKIH
                     EPPPKPQPPQQQPSNEQR"
     gene            2150..2722
                     /locus_tag="VEJ00_07810"
     CDS             2150..2722
                     /locus_tag="VEJ00_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXX01103.1"
                     /translation="MPKQSALARARKIKLLLFDVDGVLTDGKLFFPAISGNEQVGSIE
                     VKGFHAHDGVAISLARLAGLKTGLITKRTSNTVALRARDLKLEYVRQGIRDKGSAFDE
                     IVKEAGIRPDEAAFVGDDVVDLPAMRPAGLAIAVKNARAEVKKEAHFVTPHAGGDGAL
                     RDAVEFILKAQGKWKRVLEEYIRHGTASAQ"
BASE COUNT          642 a         1014 c          871 g          563 t
ORIGIN      
        1 ttacgaacac cagcgtcgaa cgatgctcgc ccaccagcgc caccagccgg tcgtaaatcg
       61 tgtcccacat ctcgtttgtc gcgatcggcc ccagcggcat cggcggcact tcgacggcga
      121 gatcgagctt ccgcttgtgc ccgatgttga cgatgacggg atcggcacgg ccgctaccgg
      181 tcaggaaatg cgccacttct tcgattggct tctgcgtcgc cgagagcccg atacgcaccg
      241 gacgcgcatg cgccagcgca tccagccgct cgagcgacag cgccagatgc gccccgcgct
      301 tgtcatctgc caccgcgtga atttcgtcga ctatcactgt ccccacatcc cgcagaatcg
      361 cccgacttcg atcggcggtc agcagaatgt agagcgactc cggcgtggtc acaagaatgt
      421 gcggaggccg ctttagcatc gcccgccgct cgggctgcag cgtatcgccc gtgcgcaccg
      481 cggtgcgaat ctccggcatg agcaacccgc gctcaccagc aagagcgaga atctctccca
      541 gcggcacttc caggtttttc tggatgtcgt tgcccagcgc cttcagtggc gaaacgtaga
      601 ggacttccgt tcggtccgcc agatccccgg ccagcgcctt gcgcaccaac cggtcgatgc
      661 aggcgagaaa cgcggcgaga gtcttgcccg acccggtcgg cgccgaaatc agcgttgtgc
      721 gcccggctag aatatgcggc cagccctgtt cctggggctc ggtcggcgtc ccaaaccgcc
      781 gcacaaacca ctccgccacc agaggatggg cccaggccag ggaggaaggc agggactgca
      841 tccgcttatt ctagatcgaa atcctgcttt cgccaactat tcgcctgtgc gaggaacgcg
      901 ccccatccac gacaggtatc atgagccaag tatcattagc gaggacgacc ggcaacgcag
      961 accaaatcat ccgctttcgg gccgcagggt agacaaattc ccttggctcg cgtcttcagg
     1021 aggtcggaat gagaaagatt ctctgggcaa ctgctgcact tcttcccatg ctcgccagcc
     1081 cgctgctctt tgcccagggg ccgcatcatt tcgagcccgg cttcaacctg ttttccaagg
     1141 agcaggatgt ccagctgggc gaggaagctg ccgcgcagtt ccgcaaacaa atgactgtgg
     1201 tcaatgaccc cgatctgacg gcctacgtca acagcgtggg caagcggctg gcggcgtcac
     1261 gcgaagcgca gggcagcggc ttccccttca ccttcgaagt tgtcgccgat cccaccatca
     1321 acgcttttgc tcttcccggc ggacacatgt tcatccacac cggcttgctg aaagccgtcg
     1381 acaacgaggc gcaactcgcc ggcgtcatgg gacacgaaat gtcgcacgtc atcttgcggc
     1441 acggcacaag ccaggcatcg aaggcgaacc tgatcgagct cccgctcatg ctggcgcaac
     1501 agataacgaa caaggattcg atgatgggca aactggagca actcggcatt ggcctgggag
     1561 ccaccggctt gttgctcagg ttctcgcgcg gcgccgagag ccaggccgac ctgaccggct
     1621 ctcacctcat ggccgaatcc ggatacgacc ctatcgaaat ggcgcacttc ttcgacaagc
     1681 tcagcggtcc cggcaagggg ctggtgggca acgtggaaca gttcatgtct gaccacccca
     1741 atcccggcaa ccgcgagcag gccattgaag acgaagcccg cctcctgccc gcgcagaagt
     1801 acggctatga gaccggcgac ttcaaacgga tgaaatcgat cgtgaagaaa atccacgaac
     1861 cgccgccgaa gccccagccg ccacagcagc agccgtcaaa cgagcagcga tagcggcgag
     1921 acagacccat gccgcgcatt cgagtccgag ttcacaaccg tggtgctgag ttaaccgccg
     1981 gccgtttgaa tcgtttgagt ttctaagccg aggctgggat gttcgggtgc tggaagggct
     2041 ttcactgctc gccattgcgc tcaatgacca atccccagcg atcccaagca atttctctct
     2101 cccactcgca ttcattccgc ccttcgccgc cctgctaaaa tccctcccga tgcccaaaca
     2161 gtctgcactt gctcgcgccc gcaaaatcaa actcttgctc ttcgacgtag acggcgtcct
     2221 caccgacggc aagctgtttt ttcccgccat ctcgggaaac gagcaagtcg gttcgatcga
     2281 agtcaaaggc tttcacgccc acgacggagt tgcgatttct ctggcgcgcc tggccggact
     2341 gaagaccggg ctcatcacca aacgaacctc caacacggtg gcgttgcgcg cgcgtgacct
     2401 gaagctcgaa tatgtgcgcc agggaatccg cgacaaaggc tctgcgttcg acgagatcgt
     2461 caaggaagct ggcatccggc cggacgaagc cgcattcgtc ggcgacgacg tcgtagatct
     2521 tcccgccatg cgccccgccg ggctggccat cgcggtcaag aacgcgcgcg ccgaggtgaa
     2581 gaaggaagcc cacttcgtca cgccgcacgc cggcggtgac ggcgccctgc gcgacgccgt
     2641 tgaattcatt ctcaaggccc agggaaagtg gaagcgcgtc ctggaggaat acatccggca
     2701 cggcactgcc tcggcacaat aacttggggt ctctcgttag tctccgacct ggctctcaga
     2761 ttcctttaac aatggcgtct tagcaacgtt tttgcaaagc ggtgacaatc cccgcgacag
     2821 gtgcaggtcc cggcgggcgc gacgactggg aacgtcgtcg tgaccgcctt cttccggaag
     2881 agcaacgcca agaccttcac cgtcacgaag taagtatgcc gcaaggcctt gaatggaccc
     2941 acggtaatga tgggtccatt ctttttggac acctcgccga ctccccttcc tgtctttagt
     3001 gatgaaaaat caggttattc aggcccgaca acccaaaaaa caaaaagccc gccggatgct
     3061 tgcttggggc gtgctcgggt cagcgtccgg
//
LOCUS       DASPUL010000317         2637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1421887, whole genome shotgun sequence.
ACCESSION   DASPUL010000317 DASPUL010000000
VERSION     DASPUL010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1421887"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VEJ00_07815"
     CDS             <1..1254
                     /locus_tag="VEJ00_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXX01104.1"
                     /translation="PGPVVIDIPTDMLKAKKEFSGPVKFTPHARPAEARAEGGFTDTI
                     VAMLQQASKPVALVGAGAKLSGAIPDLRRLLDDLNIPTFATVHGLGAVPPQAEYYLGM
                     VGMHGTRAANMALHETDLLLVFGARLDDRVTGDPSRFAPHAKIVHFEIDPAQLDRVRP
                     CDLAVIGDLADTIPAFQAELRRHSFPDFSAWRGVACGPDRAELDPRGLAQPTIRFLDE
                     LFSRLPENNAILADVGQHQMWAAQRFRSASPRGFITSGGLGAMGFALPAAVGVQLAKP
                     DTCVLCVSGDGGFQMNIQELATVHRLALPIKMVIIDNKYLGMVRQWQQLFYEKNYAET
                     DLSDNPDFVEIAKAYKISACRLNEEAMREFPVSTDTGDLIDSFLRSPEPELLVFDCHP
                     EANVFPMVPAGAALSEMLLEDEPTH"
     gene            1319..1621
                     /locus_tag="VEJ00_07820"
     CDS             1319..1621
                     /locus_tag="VEJ00_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01105.1"
                     /translation="MQAFQIRYRNTQGTLMRILNAASRRGLDLHSVHAEASEHDHLVT
                     LVLDVNPKQVGQLYREWYAIADVVHVRSAPAHSEAWVTAHPPTGVTLDEARSAARA"
     gene            1841..>2637
                     /locus_tag="VEJ00_07825"
     CDS             1841..>2637
                     /locus_tag="VEJ00_07825"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXX01106.1"
                     /translation="MDLKHISRGITDGRDRAGARAMFKAIGFTDADLSRPLIGVANTW
                     IETMPCNFHLRRLSAKVKEGVRAAGGTPMEFNTIAISDGETMGTEGMRASLVSRELIA
                     DSIELVCRGQMFDAVVCVVGCDKTIPAAAMALARMNLPGMVLYGGTIAPGNYRGRDVT
                     IQDVFEAVGANAAGKITDKELHDLEDVACPGAGACGGQYTANTMSTVMEMIGLSPMGF
                     NSVPAMDAEKDQVAFDCGRVVMNVLQQGLKPRDILTRPAFENAIASVA"
BASE COUNT          499 a          748 c          864 g          526 t
ORIGIN      
        1 cccgggccgg tcgtgatcga cattcccacc gacatgctga aggcgaagaa ggaattctcc
       61 ggtccggtga agtttacgcc ccatgcgcgg ccggccgaag ccagggccga gggtggcttc
      121 accgatacca tcgttgccat gttgcagcag gcatcgaagc cggttgcttt ggtcggcgcg
      181 ggagccaagc tctcgggagc gattccggac ttgcggcgcc tgctggacga tctgaacatt
      241 ccgaccttcg ctacggttca tggattggga gcagtcccgc cgcaggccga gtactacctc
      301 ggcatggtcg gcatgcatgg aacgcgggcg gcgaacatgg cactgcacga gaccgacctg
      361 ctgctggtgt tcggcgcgcg cctcgatgac cgtgtgaccg gcgatccttc gcgctttgcg
      421 cctcatgcca agatcgttca ttttgaaatc gatccggcgc aactggaccg cgttcggcct
      481 tgcgatctgg cggtcatcgg ggatctggcg gatacgattc cggcatttca ggccgaactg
      541 cgacgtcatt cctttccgga cttcagcgcc tggcgcgggg tggcttgcgg gccggatcgt
      601 gctgaactcg atcctcgcgg gctggcgcag ccgacgattc gttttctgga cgaactcttc
      661 agccgactgc cggaaaacaa cgccatcctc gccgacgtgg gccagcacca gatgtgggct
      721 gcgcaacgct tccgctcggc atcaccccga gggtttatca cctcgggcgg gctgggggcc
      781 atgggcttcg ccctgccggc ggcggtcggc gtgcagctgg cgaagcctga cacgtgcgtg
      841 ctttgcgtct ccggggatgg cggatttcag atgaacatcc aggaattagc cacagttcac
      901 aggctcgcac tgcccatcaa gatggtcatc atcgacaaca aatatctggg catggtgcgg
      961 cagtggcagc agttgtttta cgagaaaaac tatgccgaga ccgacctgag cgacaacccg
     1021 gatttcgtag agatcgcgaa ggcctacaag atcagcgcgt gccgcctgaa tgaggaagcc
     1081 atgcgggagt ttccggtctc aaccgacacg ggcgatctga tcgacagttt cctgcgctcg
     1141 ccggagccgg agttgctggt tttcgattgt catcccgagg cgaatgtttt tccgatggtt
     1201 ccggcgggag cggcgttgtc ggagatgttg ctggaggacg aacctacgca ctagaagggc
     1261 ggccaggatg ggcgctcgac agccggcggg acgccggcgc taccaagggg gttggcatat
     1321 gcaggctttt cagattcgct atcgcaatac ccagggcacg ctgatgcgca tcctgaacgc
     1381 ggcttcgcgc cggggactgg acttgcactc cgttcacgcg gaagcgtccg agcacgacca
     1441 tctggtgaca cttgtgctcg atgtgaatcc aaagcaggtg ggccagcttt accgcgaatg
     1501 gtatgcgatc gccgacgtgg tgcacgtgcg ctctgccccg gcacatagtg aggcctgggt
     1561 gacagcgcac cctccgacag gagtgaccct ggatgaggcg cgaagcgccg cccgggcgta
     1621 gtgtgagtgg atttactggg caagcatgac ggttacctga gcagcgtcat gcgatttggg
     1681 gaacccgcgg gaaggcgggt tccccgtttt tgtggagacg aggtttcaaa gtttcagaag
     1741 tttcgaggtt tcaacgctat agccgctgac cgtctttgaa accttgaaac tttgaaacct
     1801 gactgactcg tcctgtactc ttcaggcgag ccttatcgct atggatctca agcacatcag
     1861 ccgtggaatc actgacgggc gcgaccgcgc gggggcgcgc gccatgttca aggcgatcgg
     1921 attcactgac gccgacctta gccgtcccct gatcggagtg gccaacacct ggatcgaaac
     1981 catgccctgc aattttcact tgcgccggct ctcggccaag gtaaaggaag gcgttcgcgc
     2041 cgccggcggc acgccgatgg agttcaatac catcgcgatt tccgatggcg agaccatggg
     2101 cacggagggc atgcgggcgt cgctggtaag ccgggaattg atcgccgatt ccatcgaact
     2161 ggtttgtcgc gggcagatgt tcgacgcggt cgtctgcgtg gtgggctgcg acaagactat
     2221 tccagcggcc gctatggctc tggcgcggat gaatttgccg ggaatggtgc tctacggcgg
     2281 cacgatcgcg ccggggaact atcgcgggag agacgttacg attcaggatg tgtttgaggc
     2341 ggtcggggcg aatgccgcgg gcaagattac ggacaaagag ctgcacgatc tggaagatgt
     2401 cgcgtgcccc ggagcgggcg cctgcggggg gcaatatacg gcgaacacga tgtcgaccgt
     2461 gatggagatg atcggacttt cgccaatggg cttcaacagc gtgcccgcaa tggacgcgga
     2521 gaaggaccag gttgccttcg attgcggccg agttgtgatg aatgtcctgc agcaaggact
     2581 gaaacctcgc gacattttga cacgacctgc atttgagaat gccatcgcgt ccgtcgc
//
LOCUS       DASPUL010000318         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1422926, whole genome shotgun sequence.
ACCESSION   DASPUL010000318 DASPUL010000000
VERSION     DASPUL010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422926"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VEJ00_07830"
     CDS             <1..597
                     /locus_tag="VEJ00_07830"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXX01107.1"
                     /translation="PDMGDAGLNAFVRQFFPQIRKQGMIIDVRYNGGGFVDQLIFERL
                     RRILVGMGAARNFESTTTPPVVFHGYMVCITNQYAASDGDIFSEFFKVYKLGPLIGER
                     TWGGVRGIRGDIPLIDGGYITRPEFADYDLNSKWVVENHGVAPDIEVDNRPDDVVRGK
                     DAQLDRAIEEVMKQIQANPKKLPPRPPDLPAYPEGPAM"
     gene            764..1954
                     /gene="serA"
                     /locus_tag="VEJ00_07835"
     CDS             764..1954
                     /gene="serA"
                     /locus_tag="VEJ00_07835"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:NF008759.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXX01108.1"
                     /translation="MKVLLLESISAVAVAAFGDAGYDVESHKAALDERELIEKVHGSS
                     ILGVRSKTHVTAAVLAAAPKLVAVGAFCIGVDKIDRAAASERGVAVFNDPHSNSRSVA
                     ELVMGEIILLLRRTFQASTEMHRGVWNKSATGAHEVRGLTLGIVGYGKIGSQVSDLAE
                     AMGMRVVFHDVAHVLARGNARPMPFRELLEMSDVVTLHVDGKPQNQNLFGKEEFAAMK
                     EGVCFLNLSRGFVVDHEALAVCLKSGKLAGVAVDVFPKEPESGTGFKSVLQGLPNVIL
                     TPHIGGSTEEAQQNIGSFVSARLVDYVRTGNSMLSVNLPHCHLEYEPGSHRLMHIHHN
                     VPGILRAINDILADRGINIERQVLDTRGALGYAIYDINRPCDDGLMRLLRAVPHTIAV
                     RTGC"
     gene            2085..>2641
                     /locus_tag="VEJ00_07840"
     CDS             2085..>2641
                     /locus_tag="VEJ00_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX01109.1"
                     /translation="MRGLKDKRVLITGGASGIGAATAARFLEEGSRVCVLDRDEKACE
                     HVKRELPALGEAIIADVTDLMQVEAAFAEAIRLMDGVDILVNNAGISIRHKFLDITPE
                     EWERVIAVNLTGVFYVAQTAARHMWERGSGVILQTASTNGVLGYPYYADYNATKAGVI
                     ELTRSMALELAPKVRVCAVAPGYVLT"
BASE COUNT          525 a          711 c          887 g          518 t
ORIGIN      
        1 cccgacatgg gtgatgccgg gctcaatgcc ttcgtccggc aatttttccc gcagattcgt
       61 aaacagggca tgatcatcga cgtgcgctac aacggcggcg gatttgttga ccaactcatc
      121 ttcgagcgcc tgcggcgcat cctggtcggg atgggagcgg ctcgcaactt tgagagcacc
      181 accacacctc cggttgtttt tcacgggtac atggtgtgca tcaccaatca gtatgcggcc
      241 tccgacggtg acatcttcag cgaattcttc aaggtctaca agctgggccc gctcatcggt
      301 gagcgcacct ggggtggagt gcgcggcatt cgcggcgata ttccgctgat cgatggcggc
      361 tacattacgc gccccgagtt cgcggactat gacttgaaca gcaagtgggt cgttgaaaac
      421 cacggcgttg cgcccgacat tgaggtcgac aatcggcccg acgacgtggt tcgcggcaag
      481 gacgcacaac tggatcgcgc catcgaggaa gtgatgaagc agattcaggc gaacccgaag
      541 aagctgccac cacggcctcc ggatctgccg gcttatccgg aagggccggc gatgtagccc
      601 actgacgaag gacagccggg tcgtatttat ccccacctaa cttgcaggtc aaagccccat
      661 ttctcgcgca aacaacgcgc gagaaatggg gcacccgttt gctcctgatc cgtttcccgc
      721 tgatacgtct gagataatcg ttctgcaccc catcggagga ttcttgaaag tcctgttgct
      781 ggaaagtatc agcgccgtcg ccgtcgctgc gtttggcgac gcgggatacg acgtcgagtc
      841 gcacaaggcg gcgctggacg agcgggagct catcgagaag gtccatggga gttcgattct
      901 gggagtacgg tcgaagacgc acgtcacggc ggccgtactg gcagccgcac caaagctcgt
      961 ggcagtgggt gcgttctgca tcggcgtcga caagattgat cgcgccgcgg cgagtgagcg
     1021 cggggtcgcg gtgttcaacg atccgcattc gaactcgcgg tcggtggcgg aactggtgat
     1081 gggtgagatc atcctgctgc tgcgccgcac gtttcaggcg tcgaccgaaa tgcatcgcgg
     1141 cgtgtggaac aagtcggcga caggagcgca cgaggtgcgc gggctgactc tgggcattgt
     1201 gggctacgga aagatcggat cgcaggtttc agacctggcc gaggccatgg gcatgcgggt
     1261 ggtgttccac gacgtagcgc acgtcctggc gcgggggaat gcgcgcccaa tgccgtttcg
     1321 cgaactgctg gagatgtcgg atgtggtgac gctgcatgtc gacggcaagc cgcagaatca
     1381 gaatctgttt ggcaaagaag aattcgccgc aatgaaagag ggggtgtgct ttctgaatct
     1441 gagccgcggg ttcgtcgtgg atcatgaagc gctggctgtg tgcctgaaga gcggcaagct
     1501 ggcgggcgtt gccgtcgatg tgtttccgaa ggaaccggaa tcggggacag gcttcaagag
     1561 tgtgttgcag gggctgccga atgtgatcct gaccccgcat atcggaggct caaccgaaga
     1621 agcacagcag aatatcggct cgtttgtatc ggcgcggctg gtcgactatg tccgtactgg
     1681 caattcgatg ctgagtgtga atctgcccca ttgtcatctg gagtacgaac cagggtcgca
     1741 ccgcctgatg catattcatc acaacgtgcc tgggattttg cgggcgatta acgatattct
     1801 ggccgaccgc gggatcaata tcgaacggca ggtactggac acgcgcggcg cgctgggata
     1861 tgcgatctat gacatcaatc gtccatgcga tgacggactg atgcggctgc tgcgtgcggt
     1921 gccgcatacg attgcggtcc gaacgggatg ctgattgtgt agggacggac gcattcgtcc
     1981 gtcccgccga agcgaagcga ggcgtgcacg ctgcgagact ggatccgagc ttcgcttgga
     2041 ccacggagga atgcgttcgt ggctacataa tctaaggagc cttcatgcgc ggactcaaag
     2101 acaagcgtgt tttgatcacc ggcggagcca gcggcattgg cgcggcgacg gccgcgcggt
     2161 ttctggagga aggatcacgg gtatgtgtgc tggatcgcga cgagaaggcc tgcgagcacg
     2221 tcaaacgcga gctcccggca cttggggaag cgatcatcgc cgacgtcacc gacctcatgc
     2281 aggtagaagc cgcgtttgcc gaggcgatcc ggctgatgga cggggtcgac attctggtca
     2341 acaatgccgg catcagcatt cgccacaagt tcctggacat tactccggag gagtgggagc
     2401 gggtcatcgc ggtgaacctg accggagtgt tctacgtggc gcagacggcg gcgcggcata
     2461 tgtgggagcg gggcagcggc gtgattctgc agacggcgtc gacgaacggg gtgctggggt
     2521 atccgtacta tgccgactac aacgcgacca aggcgggagt gattgagctg acacggtcga
     2581 tggcgctgga actggcgccc aaggtgcgcg tctgcgcggt ggctccggga tacgttctga
     2641 c
//
LOCUS       DASPUL010000319         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1013455, whole genome shotgun sequence.
ACCESSION   DASPUL010000319 DASPUL010000000
VERSION     DASPUL010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013455"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VEJ00_07845"
     CDS             complement(<1..561)
                     /locus_tag="VEJ00_07845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXX01110.1"
                     /translation="MRTESPIHYSIVVPFYNEQENIPTLYMKITEVMDAIGEPYEMVF
                     VDDGSKDNSYRVLSDIYEHDRRVNVVRLRRNFGQTPALKAGFDFARGDVVISMDGDLQ
                     HDPEEIPRFLEKIEEGYDLVSGWRAQRRDHWLMRRLPSRVANWMMAKLSGIDLHDFGT
                     TFKAYRKEILQEIQLYGELHRFIPALA"
     gene            complement(674..1558)
                     /locus_tag="VEJ00_07850"
     CDS             complement(674..1558)
                     /locus_tag="VEJ00_07850"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HXX01111.1"
                     /translation="MKNLIVNADDLGWTEGVNRGIAESHRKGLVTSTSLLANGPAFAS
                     ALAVAAANPELGVGVHLNLSDGVPTAKPNEVPGLLNASGELEGGPESLLLRIASRGLE
                     LAQVEREWDAQIRKVRDAGINPTHLDGHKHVQMLPGLFEIALRLAKKHSIRAIRVAHE
                     ESKLRTLLSSGREQKPGAVLKQGVQARGLKLLARDAREMADHSGLVTTDYFCGIAQTG
                     FLTNEGVERLLKNLPDGSTELMCHPGYADEELRRTSTRLQESRQTELQILTDSRIRKL
                     VATQGIRLISYQLMGEMA"
     gene            1670..2011
                     /locus_tag="VEJ00_07855"
     CDS             1670..2011
                     /locus_tag="VEJ00_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01112.1"
                     /translation="MSVNLRELKQVGAKYREKRRGRRMNSRVQIRLEWESGGKRHALE
                     TVTRIVNPYGCMVVLTDSLEMEQRLALTNLGTQASNAAVVVWKGNEQPEGWEYGVELI
                     APDMDFWGLEL"
     gene            2008..>2391
                     /locus_tag="VEJ00_07860"
     CDS             2008..>2391
                     /locus_tag="VEJ00_07860"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXX01113.1"
                     /translation="MTPLTKPNAPGQKANAPAAAPAAGAQVKRGANAARPQTNEERRR
                     AQRVLLRMPILVYLPGKTNPLNGFTHTVSASGAMIILPEGLSQGTKLSLENPKTQKKV
                     EAHVVRPPQMNPEGSLIPVEFTAPSP"
BASE COUNT          474 a          663 c          768 g          486 t
ORIGIN      
        1 ggcgagagcc gggatgaagc ggtgcagttc gccgtagagt tgaatttcct gcaagatttc
       61 tttgcgatag gccttgaagg tagttccgaa atcgtgaagg tcgatgccgg aaagcttggc
      121 catcatccag ttggccacgc gacttggcaa gcggcgcatg agccaatgat cgcggcgctg
      181 tgctctccag ccgctgacca ggtcgtatcc ctcttcaatc ttttcgagga accggggaat
      241 ctcttcgggg tcgtgctgca ggtcgccgtc catcgaaatc acgacatcgc cgcgagcgaa
      301 atcaaagccg gccttcagcg caggagtttg tccgaagttg cggcgcaggc gcacgacgtt
      361 gacgcgccga tcgtgctcgt agatatccga aagcacgcgg taggagttgt ccttgctgcc
      421 atcatcgaca aagaccatct cgtacggctc accgattgca tccatgacct cggtgatttt
      481 catgtacaag gtagggatgt tttcctgttc gttgtagaag ggaacaacga tggagtaatg
      541 gatgggtgac tcggttcgca tacgccgtgc tatcgtgcct ttctagtgct ctccccccag
      601 catgtgccgg agtctgccgc ccttgggttg atcagtgctg gaatggttgg ccttcacgcg
      661 cccctgcgcc aggtcaggcc atttccccca tcaattgata gcttatcagg cgaatgccct
      721 gcgtggcgac aagttttctg atgcgcgagt cagtcagaat ctgcagctcg gtttgccggg
      781 attcctggag acgggtgctg gtccgccgca gctcttcatc ggcgtagccg gggtggcaca
      841 tcagttcggt ggagccgtcg ggtagattct taagaaggcg ctccacacct tcgttcgtca
      901 ggaaccctgt ctgcgcgatg ccacagaagt agtcggtggt gacgaggccg gaatggtcgg
      961 ccatctcgcg tgcatcgcgc gccaggagct tcaggccccg cgcctgcact ccctgcttca
     1021 ggacggcgcc tggtttttgc tcacgacccg aggagagaag cgtgcgaagt ttcgactcct
     1081 catgtgcgac ccggatggcg cgaatggagt gcttcttggc cagccgcaaa gcgatttcga
     1141 aaaggccggg gagcatttgg acgtgcttgt ggccgtcgag gtgggtgggg ttaatgcctg
     1201 catcccgcac tttgcgaatc tgggcgtccc attcgcgctc cacctgggct agttccaaac
     1261 cgcggctggc gatacggagc agcaaggact ccgggccgcc ttccagttca cccgacgcat
     1321 tgagcaggcc aggcacttcg ttaggcttgg cggtgggaac accatcgctg aggttcaggt
     1381 gaacgccgac cccgagttcg gggttagcgg cggcgactgc aagcgccgag gcaaaggccg
     1441 gaccgttagc cagcagggaa gtgctggtca cgagtccttt gcggtgggat tcagcgattc
     1501 cacggttgac gccctcggtc cagccaaggt cgtccgcatt gacgatcagg tttttcactt
     1561 caggaagtgc tcctgtccgc aaggacagct actctttcga cggcggtccg gcattcgtac
     1621 cattgaccat gattcgtctt aactttagca tgaccagcta gaggccagga tgtccgttaa
     1681 cctgcgcgaa ttgaagcaag taggggcaaa atatcgggaa aagcgccggg gacggcgcat
     1741 gaattcccgt gtgcagatcc gtttggagtg ggagtccggc ggaaaacgtc atgcgctgga
     1801 gaccgtcacc cggatcgtga acccctatgg ctgcatggtg gtgctgaccg attcgctgga
     1861 gatggagcag cgtcttgccc tgacgaatct tggcacgcaa gccagcaatg cggcggtcgt
     1921 cgtttggaaa gggaacgaac agccggaagg ctgggaatat ggcgtggaac tcatcgcgcc
     1981 ggatatggat ttctgggggc tggaactgtg acacctctta cgaagccgaa tgctcctggc
     2041 cagaaagcga atgcgccagc agccgctccc gcggcaggcg cgcaagtgaa acgtggcgcc
     2101 aatgccgcgc ggccgcagac gaacgaagag cgacgccggg cacaacgcgt gctgctgcgc
     2161 atgccgatcc tggtgtatct gccagggaag acgaatccac tgaacgggtt cacgcacaca
     2221 gtgagcgcga gcggggcgat gattatcctg ccggagggac tatcgcaggg cacgaagctg
     2281 tcgctggaaa atccgaagac gcagaagaag gtagaggcgc atgtggtgcg gcctccgcag
     2341 atgaacccgg agggatcgct gatcccggtg gagtttacgg cgccatcgcc g
//
LOCUS       DASPUL010000320         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_888041, whole genome shotgun sequence.
ACCESSION   DASPUL010000320 DASPUL010000000
VERSION     DASPUL010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888041"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /gene="ubiE"
                     /locus_tag="VEJ00_07865"
     CDS             <1..701
                     /gene="ubiE"
                     /locus_tag="VEJ00_07865"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HXX01114.1"
                     /translation="RAVREMFTSVAPRYDLLNHVLSFNVDRLWWRRAARTFRHILNVP
                     EARVLDLCCGTGDMTFALRREAGKPSPRIFGADFSHAMLQRASAKAQASARCGTNPDW
                     IEADALNLPFPSEHFDLVTSAFGFRNLADYDAGLCEIVRVLRPGGECGILDFGEPKGA
                     MGALYRIYFRQVLPRVGTLVSGVRGPYAYLPASVERFPDPQEMVERMKRAGFAEASWT
                     PYTFGIAGLYRGKK"
     gene            complement(744..1493)
                     /locus_tag="VEJ00_07870"
     CDS             complement(744..1493)
                     /locus_tag="VEJ00_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXX01115.1"
                     /translation="MGCEVWRGKLDAYLDGELPTAEAAALAAHLRECASCAADALGRV
                     QIKRAVAAAGRRYEPSAQLRARIARGVAAQPQKKAARWSLLWAPVFTVAIVAFAVAFY
                     LGREGTRQQRVYSELADLHIGALTSATPVDVVSTDRHTVKPWFQGKIPFTFNLPELQG
                     TDFTLLGGRVTYLAQTPGAHLIYQYRKHEISVFIFQDRGEETAGVPSGPAHAQSFNFA
                     SWTENGLRYFVVGDVSADQIQALSKLLRDAG"
     gene            complement(1483..2007)
                     /locus_tag="VEJ00_07875"
     CDS             complement(1483..2007)
                     /locus_tag="VEJ00_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXX01116.1"
                     /translation="MTATTFEDLALPLFDQLYNFARWLTQDTSEAEDLVQETYAKALR
                     GFPSFQVGTNFRAWMYRILKNSFLSSRTGLKASVPLDEEDPDNVPAAEGPTPESLVIQ
                     QSNRELVQKALEELPIHYREILLLCEVEEMSYQEISEALSIPVGTVMSRLFRARKALR
                     GLLQQKLQGGRHGM"
BASE COUNT          427 a          719 c          706 g          442 t
ORIGIN      
        1 cgcgggccgt gcgcgagatg ttcacctcgg tcgccccgcg ctacgacctg ctgaaccatg
       61 tgttgtcgtt caacgtggac cggctgtggt ggcggcgggc ggcgcgcacg ttccgccaca
      121 ttctcaacgt cccggaggcg cgcgttcttg acctgtgttg cggcacgggc gatatgacct
      181 tcgctctgcg ccgcgaggca gggaagccct ctccccggat tttcggcgcg gatttctccc
      241 acgctatgct gcagcgggcc agcgccaaag cgcaggcctc cgcgcgatgc gggacgaacc
      301 ccgactggat cgaagctgac gccctgaacc tgccatttcc ttccgaacac ttcgatctgg
      361 tcacctcggc cttcggattc cgcaatctcg ccgactacga tgccgggctg tgcgagattg
      421 tccgggtgct tcggcccggc ggtgagtgcg gaattctcga ttttggcgag ccgaaaggcg
      481 cgatgggcgc actgtaccgc atttacttca ggcaggtgtt gccgcgggtg gggacgctgg
      541 tctcgggagt tcgcgggccc tacgcctatc tgccggcctc ggtcgaacgg ttccctgatc
      601 cgcaggaaat ggtagagagg atgaagcgcg caggattcgc cgaagccagc tggacgccct
      661 atacgtttgg gatcgcgggg ctgtatcgcg ggaagaaatg aatggagctt ctcattccac
      721 gtagggacag ccgcccggct gtcctagccc gcatcgcgca gcagcttgct cagagcctga
      781 atctggtctg cgctgacatc gcccacaaca aaatagcgca atccgttctc ggtccagctc
      841 gcgaaattga acgactgcgc gtgcgctggc ccggagggca cgccagccgt ttcctcgccg
      901 cgatcctgaa agatgaaaac cgagatctcg tgcttgcggt actgatagat caggtgcgcc
      961 cccggagtct gggccaggta cgtgactcgc cctccgagca aagtgaagtc ggtcccttgt
     1021 aactccggca aattaaaggt gaacgggatc tttccctgga accacggttt caccgtatgg
     1081 cggtcagttg agacgacatc aaccggcgtg gcgctggtaa gagcacctat gtgaagatcg
     1141 gccaactcgc tgtacacccg ctgctggcgc gtaccttccc gtccaaggta gaaggcgacg
     1201 gcaaacgcaa caatcgctac cgtgaagact ggcgcccaca agaggctcca tcgggcggct
     1261 ttcttttgcg gctgggcggc aacgccccgg gcaatgcggg cgcgcaactg tgcgctcggc
     1321 tcatagcgcc tgccggcggc ggccacggcg cgcttgatct gcactcgccc cagagcgtcg
     1381 gcggcacacg atgcgcattc ccgcagatga gccgccagcg cggcagcttc ggcagtcggc
     1441 agttcgccgt ccagatacgc gtcgagcttc ccgcgccaca cctcacatcc catggcgtcc
     1501 tccttgcaac ttctgctgga gtagaccccg caaggccttg cgagcgcgga ataggcgcga
     1561 catgaccgtg cctacaggaa tcgacaacgc ctcggaaatc tcctgatacg acatctcctc
     1621 aacctcgcac agcagcagga tctcgcggta gtgaatcggc agctcctcca gcgccttctg
     1681 caccagctcg cggttgctct gctggattac cagactctct ggggtcggtc cttctgcggc
     1741 aggcacgttg tccgggtcct cctcgtcgag aggtacgctg gccttgagcc ctgtccgcga
     1801 gctgaggaac gaattcttca ggatacggta catccaggcg cggaagttcg tgcccacttg
     1861 aaacgatggg aatccgcgca gcgccttggc ataggtttcc tgcaccaggt cttccgcctc
     1921 ggaggtgtcc tgcgtgagcc agcgcgcaaa gttgtagagc tggtcgaaaa gagggagggc
     1981 caggtcctcg aacgtggtag cggtcaagcg ctgatcggac acctccatag gataaaccgc
     2041 gggttcggca gtttattccc atattcccga caaacttttt gcgatgcgtc cggaatattt
     2101 ccgggcgcca ccggttttcc cgacagcaac gcagccagga gagaacaaga aacatgaaac
     2161 aaaccgatcg cagatcgttt ttgaaaattg ctggcacgtc gctgggcatc ggcgtgctct
     2221 actcggctta cccggcggca atggccaaag gccaggtggg agacatgttt gcgtcgctcg
     2281 gccgggccaa cggc
//
LOCUS       DASPUL010000321         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_202175, whole genome shotgun sequence.
ACCESSION   DASPUL010000321 DASPUL010000000
VERSION     DASPUL010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202175"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VEJ00_07880"
     CDS             <1..315
                     /locus_tag="VEJ00_07880"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HXX01117.1"
                     /translation="LLFEVIMQNPPQPAIQLTNAPDYRENYANSVQMRVNIWDFFLVF
                     GTLQQQTETQVEIRNFQGIYLSPRQAKALLGLLQQNVAGYESAFGEIKLDPRMSPAGP
                     VH"
     gene            363..2150
                     /locus_tag="VEJ00_07885"
     CDS             363..2150
                     /locus_tag="VEJ00_07885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXX01118.1"
                     /translation="MRKGLSAKIPENWWLIFAHPALTFAIILVTMFLLHAPLLQLPYF
                     WDEAGYYVPAARDFLLTGSLIPHSTLSNAHPPLVLAWLALWWKIVGYAPLVTRSAMLV
                     VAAFSLLGLFRLAERAANTQVAIASTLCTALYPVFFAQSSLAHVDLAAAGLTFWALCA
                     YLEERPIAAAIWFSAAALAKETAILAPLALAAWELLRLVASRRQEHGLSHGAADTHVG
                     PKEQRGRAALQGRVSLHQSEGALAPEVKSPRVLSLLFPLVPLILWYAYHYARTGYVFG
                     NPEFFRYNVAATLNPLRFLLALGLRLWQTFGYMHLWLLTLTMLLAMLLPPVDGETAAH
                     GDAHVGTGALARPVARSAAARQRIAIPIQCVLLVVIVAYVIAMALIGGAVLARYMLPA
                     VPLGIILAVSTLRRRLRNWPVAIAVIALAFVAGWFWNPPYGFSPEDNLAYRDYVILHQ
                     DAERFLEARYPMARVLTAWPASDELTRPWLGYTTRPVQVVRIEDFSAEEILSAADFRQ
                     SFEVALIFSTKYEPPPTLFGRWTQGRWKTWQTWKRRFFGFHRDVPPAAAAQILGGRIV
                     YSGQRRGQWVAVIEMKREEILEAGYKETN"
     gene            complement(2147..>3149)
                     /locus_tag="VEJ00_07890"
     CDS             complement(2147..>3149)
                     /locus_tag="VEJ00_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXX01119.1"
                     /translation="PSGAGKTTLLDCVAGLARPDSGKIAIGGRVLFDAARGTNIPISK
                     RHVGYVFQSLALFPHLTVEQNVRYGLAHSPRTEREVKTNAILNAFHIAPLASRFPREI
                     SGGESQRVALARTLVTDPAVLLLDEPLAALDAATKAKIIDDLRAWNRAHRIPILYVTH
                     SREEVFALGERVLVLDAGCIVAEGTPHEVMSAPLHETVAQLAGFENIFDAVVEAVRPE
                     RGTMTCRLKTPDERTPVLLETPLVRGGAGAAMRVGIRAGDILLATTVPVGLSARNVLP
                     GHIMLLEQRDVIVAARVNCRVEMEVHLTLAARDSLELGPGKEVWLVIKTHSCHLMQA"
BASE COUNT          570 a         1035 c          899 g          645 t
ORIGIN      
        1 ctgctattcg aggtcatcat gcagaatcct ccgcagccag cgattcaatt gacgaatgca
       61 cccgactatc gcgagaacta cgccaacagc gtgcagatgc gggtgaatat ctgggacttc
      121 ttcctcgtct ttggaactct gcagcaacag accgaaaccc aggtcgagat ccgcaacttc
      181 cagggcatct atctcagccc gcggcaggcc aaggcattgc tcggactcct gcagcagaac
      241 gttgccggat acgagagtgc attcggtgaa atcaagctcg acccccgcat gagcccggcc
      301 ggacccgtgc actaacaaat tctttgccac ggattcaacg gattcacgcg gattatagaa
      361 aaatgcgaaa aggactatcc gcgaagatcc ccgaaaattg gtggctgatt ttcgcgcatc
      421 cagctctcac attcgcgatc atcctcgtca cgatgttcct gctgcacgcg cccttgctgc
      481 agcttccata cttttgggat gaggctggct actacgttcc tgcagcccgc gattttctgc
      541 tgaccggcag cctgatcccg cactcaactc tctccaatgc tcatcccccg ctggtactgg
      601 cgtggctcgc gctgtggtgg aagatcgtgg gctatgcccc gctggtgacg cgatcggcca
      661 tgctcgtggt ggcggcgttc tctctgctgg gccttttccg cctcgccgaa cgcgccgcta
      721 atacgcaggt agcgatcgct tcgaccctct gcaccgcgct atatccagta tttttcgccc
      781 agagctcgct cgcacacgtg gatcttgccg ccgccggcct cactttctgg gcgctatgcg
      841 cttacctcga agagcgcccc attgcggccg cgatctggtt ctcggccgcc gccctggcaa
      901 aagaaacggc gattctggca ccgttagcgc tggcagcgtg ggagctgctg cgcctggttg
      961 cgagccgacg tcaggagcac gggctctctc atggtgccgc cgacacgcac gttggaccaa
     1021 aggagcaacg tggaagagcg gcccttcagg gccgcgttag cctccatcag tcggaggggg
     1081 ctttagcccc tgaggtgaaa tctccccgcg ttctgtctct cctattcccg cttgttcccc
     1141 tgatcctctg gtacgcctac cactacgctc gcaccggtta cgtcttcggc aaccccgaat
     1201 tcttccgcta caacgtcgcc gccacgctca accctttgcg attcctgctg gcactcggac
     1261 ttcgcctgtg gcaaaccttc ggctacatgc atttatggct gctgacgctc accatgctgc
     1321 tagccatgct gttgccaccg gttgacggag agacagccgc ccacggcgat gcccatgtgg
     1381 ggacgggcgc cctcgcccgt ccggtcgcgc gaagcgcggc ggctcgccag cggatcgcta
     1441 tcccaatcca atgcgttctt ctcgtggtca tagtcgccta cgtgatcgcc atggctttga
     1501 tcggcggcgc cgtccttgcc cgctacatgc tgccggctgt tccgttggga atcatccttg
     1561 ccgtctccac gctgcgccgc cgtctgcgga attggcccgt agcgatcgcc gtcatcgcgc
     1621 ttgcgttcgt cgccggctgg ttctggaatc ctccctacgg attttccccg gaagacaatc
     1681 tcgcctaccg cgactatgtg atcctgcacc aggatgcaga gcgtttcctc gaagcccgct
     1741 atcccatggc ccgcgtgctc accgcatggc ccgcgtcgga tgaactgacc cgcccctggc
     1801 tcggctacac cacgcgtccg gtgcaggtcg tgcgcataga agacttctcg gcagaagaga
     1861 ttctttccgc cgcggatttt cggcagagtt tcgaggtcgc cctcatcttc tcgaccaagt
     1921 acgaacctcc tccgacgttg ttcggccgtt ggacgcaagg gcgttggaaa acctggcaaa
     1981 cctggaagcg ccgcttcttc ggcttccacc gcgatgtgcc gcccgcagcg gcagcacaga
     2041 ttcttggcgg ccggattgtc tattcagggc agcgcagggg ccagtgggtg gctgtgattg
     2101 agatgaagcg ggaagaaatt ctggaggctg ggtacaagga gaccaattaa gcctgcatca
     2161 aatgacagga gtgggttttg atcacgagcc acacttcttt gccgggcccg agttcgagcg
     2221 agtcccgcgc cgccagcgtg aggtgaactt ccatctcgac ccggcaattc acacgcgcag
     2281 ctacgatgac atcgcgctgc tcaagcaaca tgatgtgtcc cgggagcacg ttgcgtgcgc
     2341 tcaatccgac cggcaccgtc gtcgccagca gaatgtcgcc agcgcggatg ccgacacgca
     2401 tcgccgcgcc tgcgccgccg cgcaccagag gagtctccag caaaaccggg gttcgctcgt
     2461 ccggggtttt tagccggcag gtcatcgtgc cgcgctcagg ccggaccgct tccaccactg
     2521 cgtcgaagat attctcgaaa ccggcgagtt gcgcgaccgt ctcgtgcagc ggcgcgctca
     2581 tcacttcatg tggcgtgcct tcggccacga tgcacccggc atcgagcacc aggacccgct
     2641 cgcccagggc aaagacttcc tcccggctgt gcgtcacata aagaatggga atgcggtgtg
     2701 cccggttcca cgcgcgcaga tcatcaataa tttttgcctt tgttgctgca tcgagcgcgg
     2761 ccagcggttc gtccagaagc agcaccgcgg gatcggtcac cagggtccgc gccagagcta
     2821 cgcgctgact ctccccgccg gaaatctcgc gcggaaaacg cgatgcaagc ggagcaatgt
     2881 gaaatgcgtt gagaatcgcg ttggttttca cttcgcgctc ggtgcgaggc gagtgggcca
     2941 gcccgtagcg gacgttctgc tccacggtga gatgcgggaa caaggcgagg ctctgaaaca
     3001 cgtatccgac gtggcgtttc gaaatcggaa tgttcgtacc ccgcgcagcg tcaaacaaaa
     3061 ctcgcccgcc aatcgcaatc tttccggagt ccggccgggc aagccccgca acgcaatcga
     3121 gcagcgtcgt cttccctgcg cccgacggg
//
LOCUS       DASPUL010000322         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_740741, whole genome shotgun sequence.
ACCESSION   DASPUL010000322 DASPUL010000000
VERSION     DASPUL010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740741"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VEJ00_07895"
     CDS             <1..261
                     /locus_tag="VEJ00_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01120.1"
                     /translation="HPPDAQLDRLFRYITLETGLLAGALLTIGGLGLSVYAVSLWGSR
                     HFGAMDYSQTMRLVIPAAVLLMLGVQTVFASFFMSVLGLRRR"
     gene            complement(292..876)
                     /locus_tag="VEJ00_07900"
     CDS             complement(292..876)
                     /locus_tag="VEJ00_07900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXX01121.1"
                     /translation="MHEKLISGRRVEVLTNWFAQLLPPGSRILDVGCGDGRLSAILQT
                     KRADLSIRGIDVLPRTHSHIPVDLFDGTRIPFPDASFNVVLFSDVLHHTADPTILLRE
                     ARRVASRHVLLKDHYRQGIAAQARLRLMDWVGNARFGVALPFNYWSVDQWHTAWRDVG
                     LRPQQLVTRLGLYPPPADWVFGAKLHFIALLDLV"
     gene            1226..2338
                     /locus_tag="VEJ00_07905"
     CDS             1226..2338
                     /locus_tag="VEJ00_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01122.1"
                     /translation="MTAPPAVVRSKPSQLEIISLAGLGFVLFISTISLARNYFSAIDN
                     FGDSMGYMNLASAIRRWDFSGIAIKQFWGLPYAMAALSKLTGASDRTALLVLSFVPSL
                     LSGLLARRLWGGWVAGYFLILNFDWLQRSVLGGSEPLFVCLLFAAFLCVRQERWMVAA
                     LLASFATVVRPLGLFALVGIGLTLLREREFRKFAAATLIGLLVGVLYAIPLASHFGDP
                     LANVNSYHNRAWQGGWLFGFPFYAIIKGTLVYPAPWTNLLLSFSWILLVLAAAFVMVR
                     SRDFRSYCRAHAAETVFLIPYLWCLFTYNYPYWARGNFARFAIPILPFVLLALDRWVP
                     KDRRVLWGLGVVTPVLAACSALGIRNVVQMLEKSLG"
BASE COUNT          443 a          721 c          710 g          563 t
ORIGIN      
        1 cacccgcccg atgcccagtt ggaccgcctc ttccgctaca tcacgctgga aacaggactt
       61 ctggccggtg ccctgttgac gatcggagga ctggggctct ccgtctatgc cgtcagcttg
      121 tggggaagcc ggcatttcgg cgcgatggac tactctcaaa ccatgcgcct ggtcattccc
      181 gcggccgtgc ttttgatgct tggagtgcag accgtgttcg caagcttctt tatgagtgta
      241 ctgggattgc gccgccgctg aatgcgcatt gtcttttgca ggatgtcgtg atcagaccag
      301 atccagcagc gcgatgaagt gaagctttgc tccgaacacc caatcggcgg gtggtggata
      361 aagacccagc cttgtcacca actgctgcgg ccgcagtccc acgtcccgcc aggctgtgtg
      421 ccattgatcc acgctccagt agttgaaagg caaggcgaca ccgaatcggg cgttgccgac
      481 ccaatccata agtcgcagcc tggcttgcgc agcaattcct tgtcggtagt gatccttgag
      541 cagaacatgc cgcgacgcca ctcggcgcgc ttcccttagc aagatcgtcg ggtccgcggt
      601 gtgatgcaag acgtcagaaa agagaacaac attaaaagag gcgtcgggaa acggaatacg
      661 ggtcccgtcg aacagatcca ccggtatgtg actgtgagtg cggggaagaa cgtcgattcc
      721 gcggatgctg aggtcggccc gtttcgtctg cagaatcgca gaaagtcgac cgtcgccgca
      781 gcctacatcg agtatccgcg atcccggagg aagcagctgc gcaaaccaat tcgtaagaac
      841 ctcgacgcgc cggccggaga tcagtttctc atgcacaacg ccgacagcgc tggcaggcag
      901 tgaatttgat ttcgaaccag gtgacagctc ggacctcctg gcctggcgag gatcctgatg
      961 gaaaggcctg aaatcccgat ccagccggca atccatagta caagggcaat acagtgaggc
     1021 caactattcg gaactggccg cgtgatgctg agcgccgtct ttatgcgccg ctccggaaca
     1081 aaggccatcg ggatggcgct ataatccgct cactcggtgc ccatgatctg acgttcaagc
     1141 gggagaaaca gagaatcgag atacgagact gatgttgccc tccggaaccc ttgctcgcaa
     1201 agcgccttct ccatccgcgg atgcgttgac ggcgcccccg gcggtcgtgc gctccaagcc
     1261 ctcgcagctg gagatcatct cgttggccgg tctcggcttt gtgttgttta tctcgacgat
     1321 ctcgctggcg cgcaactact tttccgccat cgacaacttc ggcgacagca tgggctacat
     1381 gaacctggct tcggcgatcc ggcgctggga ctttagcggc atcgcgatca agcaattctg
     1441 ggggctgccc tatgccatgg cagctctatc gaagttgaca ggggcatccg accgtacggc
     1501 tttgctggtt ttgtcgttcg ttccttcgct gctctcaggc ctgctcgccc gccgcttgtg
     1561 gggtggatgg gttgccggat atttcctgat cctgaacttt gattggctac agcgctcggt
     1621 gctgggtgga tcggagcctc tgttcgtgtg cctgctcttc gctgcgtttc tttgtgtacg
     1681 acaggagcgg tggatggtgg ccgcgctgct ggcttcattc gcgaccgtag tgcggccgct
     1741 ggggcttttc gcgctcgtcg gcataggact caccctgctc cgggagcgcg aatttcgcaa
     1801 attcgcagcc gcgactttga tcgggctgtt ggtcggcgtt ctctacgcga taccgttggc
     1861 ctctcacttc ggcgatccac tggccaacgt caacagctat cacaaccggg cctggcaggg
     1921 cggctggctc ttcggttttc ccttctacgc catcatcaaa ggaaccctcg tctaccccgc
     1981 gccctggacc aatctccttc tgagctttag ctggatcctt ctggttcttg cggcggcgtt
     2041 cgtgatggtg aggtcccggg actttcgctc ttattgccgc gcgcacgcgg cggagacggt
     2101 atttctcata ccgtatcttt ggtgcctgtt cacctataac tacccgtact gggcccgtgg
     2161 caatttcgca cgcttcgcca tccccattct gcctttcgta ttgctggctc tcgaccgttg
     2221 ggtacccaaa gaccgccgcg tgctctgggg gctaggtgtg gtaactcctg tacttgctgc
     2281 ctgctccgct ctaggcatca ggaacgttgt ccagatgctt gaaaagtctc ttggttgact
     2341 tcgggaaaga aaaaaccgcc tggacatcgg gttgccgcac cctgcattct atgtatggtc
     2401 cgactggtgg cagtgtgaca gcatgcggta gtcgaca
//
LOCUS       DASPUL010000323         4098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_330555, whole genome shotgun sequence.
ACCESSION   DASPUL010000323 DASPUL010000000
VERSION     DASPUL010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330555"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..1153)
                     /locus_tag="VEJ00_07910"
     CDS             complement(266..1153)
                     /locus_tag="VEJ00_07910"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXX01123.1"
                     /translation="MTRLHFSAGSRSLAVLLSSLLVFAAALRAQDQIVWSDQEKQIVE
                     QIKSLRNLDDATRARTTRGLARQIGQLPADPNKVQLAGALASRATEGDFGRDTLQEVT
                     TTLATALREQPPGREKDGGPNPAFVELAELVRYEHMSATSSDPQFAEATAKLEAADAA
                     RQKADFTLADLHEKTWHLQDLRGKVVLVNFWATWCPPCRKEMPDLQSLYDKYKNQGFV
                     VLAISDEEAAKVAPFIAERNISYPILLDPGRKVHELYQVEGIPKSFVYDRDGKLVAQS
                     IDMRTRSQFQEMLAQAGLR"
     gene            complement(1191..1709)
                     /locus_tag="VEJ00_07915"
     CDS             complement(1191..1709)
                     /locus_tag="VEJ00_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01124.1"
                     /translation="MDKLSWFILFTGIAVMLQAGILAAMYFTMRKSSERMAALATEVK
                     TKVLPTVDQVQTVLTDIRPKIQTIADNLQESTTVVRTQVQRMDAAVSDVVDRARLQVI
                     RADELMTRTLDRVEQTSDMVHKTVVSPVRQLSGLMQGITVGLEFLFGGRGRKNGDRRE
                     ERRPVPQDEMFI"
     gene            complement(1765..2073)
                     /locus_tag="VEJ00_07920"
     CDS             complement(1765..2073)
                     /locus_tag="VEJ00_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HXX01125.1"
                     /translation="MSDRDGNSFVWFLAGLGLGALVGVLYAPRSGSETREVLRARAEE
                     GRDYVRSRAREAREQASQWADRGREVMSQQKEQFRAAYEAGRQAYHEATTETGAPKNL
                     "
     gene            complement(2270..2593)
                     /gene="secG"
                     /locus_tag="VEJ00_07925"
     CDS             complement(2270..2593)
                     /gene="secG"
                     /locus_tag="VEJ00_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXX01126.1"
                     /translation="MIYLITTVHVLVCFFIILVVLLQSGKSGDISAAFGGQGSQTAFG
                     PRGAATALSKATTWSAVIFMITSITLAVFATKRGGPSSLLQGVKSQPVKTQPAPAQKP
                     AAPAK"
     gene            complement(2713..2797)
                     /locus_tag="VEJ00_07930"
     tRNA            complement(2713..2797)
                     /locus_tag="VEJ00_07930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2761..2763),aa:Leu,seq:gag)
     gene            complement(3045..>4098)
                     /locus_tag="VEJ00_07935"
     CDS             complement(3045..>4098)
                     /locus_tag="VEJ00_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HXX01127.1"
                     /translation="NNALQRADQVHHAEGKVAPGETWFAPLVADAEAGFGGTLNAFEL
                     MKGMIEAGAACVHFEDQLSSAKKCGHLGGKVLVPTTEAVQKLVAARLAADVMGVPSLI
                     MARTDADSAHLLTSDIDSRDRAFCTGERTSEGFFRIRGGIQSAIARGLAYAPYADLIW
                     CETSHPDIEEARQFAEAIHAKYPEKMLAYNCSPSFNWRKNLDEATIAKFQTELGRMGY
                     KFQFVTLAGFHALNLSMFELARGYKLAGMTAYSRLQEKEFSRETQYGYEAVKHQRFVG
                     TGYFDQVQQVITGGLASTIALAESTEAEQFTERKALVHPKQGPDAGCQPILGECPHVP
                     VTIDPGPEEMLLPSGD"
BASE COUNT          739 a         1326 c         1188 g          845 t
ORIGIN      
        1 gcgcggtctg gtcggcgcac caccgggctt cctgcatgtt gcgcaggtat tcgccctcag
       61 cgacggagcc tcgccccaga gtctcctgct cgatggcttc taacagatca ccttcccgcc
      121 cgcgtctcag tcgcgcccgc accagatatc gcatattttt gtctgtcatc ccacgcaagc
      181 gctttttgcg ccgcgaggga cgtgggcgag ccgcgcgaag cgtcgcgcta ttgcgacgca
      241 gtcatcgcgc gtttggctcg tttctttacc gcaaacccgc ctgtgccagc atttcctgaa
      301 actggctccg ggtgcgcatg tcgatcgact gtgcgaccaa cttgccgtcg cggtcgtaga
      361 cgaagctctt gggaatccct tcgacttgat aaagttcgtg cacttttcgc cccggatcga
      421 gcaggatggg gtagctgatg ttacgctcag caataaatgg cgcaaccttg gcagcttcct
      481 cgtcagaaat cgccagaacc acgaagccct ggttcttgta tttgtcatac aaagactgga
      541 ggtctggcat ttccttgcgg cagggcgggc accatgtcgc ccagaaattc acgagcacaa
      601 ccttgccccg cagatcctgc agatgccatg tcttttcgtg aagatcagcg agcgtgaagt
      661 cagctttctg gcgggcggca tccgcggcct ctagtttcgc tgtggcttcg gcaaactgcg
      721 ggtcgctcga cgttgcgctc atatgctcgt agcggacgag ttcggccagc tccacgaagg
      781 cggggtttgg cccaccgtct ttctcccgcc ccggaggctg ttcccgcagc gctgtggcca
      841 gagtcgtggt gacctcctgc agcgtgtcgc ggccgaagtc gccttccgtc gcacgactgg
      901 ccaaagcgcc tgccagctga actttgttgg gatcggcggg caattgcccg atctgccgcg
      961 cgaggcctct ggtcgtgcgt gcgcgggtgg cgtcgtccag attgcgaagg ctttttatct
     1021 gctcaacaat ctgtttttcc tggtcgctcc agacgatttg gtcctgtgct cgcagtgcgg
     1081 cggcgaacac aagcagagaa ctcagcaaaa cggcgagcga gcgtgaaccg gcggaaaagt
     1141 gaagcctagt catctaggag ttcccacgcg gaggaagaac aatgcttcgg tcagatgaac
     1201 atctcgtcct gcggcaccgg acgccgttcc tcgcgccggt cgccgttctt gcgtccgcgg
     1261 ccgccgaaca ggaactccag ccccacagta attccttgca tcagtccgga gagttgccgc
     1321 accggcgaga ccacggtttt gtgcaccatg tcggaagttt gttcgacccg gtccagcgtg
     1381 cgtgtcatca gttcgtcggc gcgaatgacc tgcagccggg cgcgatcgac cacatcactt
     1441 acggccgcgt ccatgcgctg tacttgtgtg cgcacgaccg tcgtggactc ctgcagattg
     1501 tcggcgatcg tctgaatttt cgggcggatg tcggtcagca ccgtctgcac ctgatcgacc
     1561 gtagggagca ctttcgtttt cacctccgtc gccagggccg ccatacgctc gctgctcttg
     1621 cgcatggtga agtacatcgc cgccaggatg cccgcctgca gcatgaccgc aatgccggta
     1681 aacaagatga accaactcag cttgtccata agttatgacc ccgccctctg cctacctgcc
     1741 agggaaggat gagtcggcac gcgcctacag attcttcggt gcgcccgtct cggtcgtggc
     1801 ctcgtgatac gcctggcggc cggcttcata cgcagcgcga aactgctcct tctgctgact
     1861 catcacttcg cggccgcgat cagcccactg cgacgcctgc tcccgcgctt cgcgcgcgcg
     1921 gctgcgaaca tagtcgcgtc cttcctcagc gcgcgcccgt agcacctccc gggtctcgct
     1981 gcccgagcgc ggcgcataca gcacgccgac cagcgccccc aatcccagtc ccgccagaaa
     2041 ccacacaaaa ctgttgccgt cacgatccga catgttggta cctccctatc atggccttaa
     2101 agcggatggt agcaccccga atcgtggaat acaattccgc tccgcaagcg gcggtaacgg
     2161 aaattccccg accgtaaagc gcacaaaatc gcaggctatc cggcattcgc cggcctgcct
     2221 tcgtgtgtct tgtcttcgtg ctcgtagtgg tgaactggcc tagctcgatt tacttggcag
     2281 gcgcggccgg cttttgcgcc ggtgcaggct gagtcttcac cggctgcgac ttcactccct
     2341 gcaataggga gcttggacca ccccgcttgg ttgcgaatac tgccagcgtg atggaggtga
     2401 tcatgaaaat caccgctgac caggtagtcg ccttcgaaag tgccgtcgcc gctccgcgcg
     2461 gaccaaatgc cgtctggctg ccctgtccgc cgaacgcggc cgagatgtcg ccgctcttgc
     2521 cgctctgcag caacacgacg agaatgatga agaagcagac cagaacgtga accgtcgtaa
     2581 tcagatagat cataagccga cttttgaggg cgcaccgatc cgcgcctcgc ttacgctccc
     2641 tagggtgttc gatcggatcg tctgcgggag agcccttggc gacgactgcc ggcgaacaga
     2701 aattgaaaat aatggtgcgg aaggggggat ttgaaccccc acgcctttcg gcgccacccc
     2761 ctcaagatgg tgtgtctgcc agttccacca cttccgcaca aatgtgaaac tgcactgccc
     2821 ggcagcactt cattatagca aagcagaatt gccgggcagg gtcgcagctt ttctctgcgc
     2881 tctttgcggc atttcttcgt gaactctgcg ataagtcttt ttagccgata agaccagcgg
     2941 gtcgtcggcg gccagcgacg atcgccgcga tccgcacagc aaaaaaaaat gccggcctgt
     3001 gctacaggcc ggctcgtgat gcagcgtcac cgctgtgaga gaagttagtc gccggagggc
     3061 agcagcattt cctccggtcc cggatcgatc gtgaccggca catgcgggca ctcgccgagg
     3121 atcggctggc agcctgcgtc cggcccttgc ttgggatgaa ccaacgcctt ccgctccgtg
     3181 aactgctcgg cctcggtaga ctccgccagt gcgatcgtcg acgccaggcc gccggtgatc
     3241 acctgctgca cctggtcgaa gtaccccgtg cccacgaagc gctggtgctt caccgcctcg
     3301 tagccgtact gcgtttcacg gctgaattcc ttttcctgca gccgcgaata cgctgtcata
     3361 cccgcgagct tgtagccgcg cgccagctcg aacatgctga gattcagcgc atggaagccc
     3421 gccagcgtga cgaactggaa cttgtagccc atgcgtccga gttccgtctg gaacttcgca
     3481 atggtcgctt cgtccaggtt cttccgccag ttgaagctgg gcgagcagtt gtaggccagc
     3541 atcttctccg gatacttggc gtgaattgcc tcggcaaatt gccgcgcctc ctcgatatcc
     3601 ggatgcgaag tctcgcacca gatcaggtcg gcgtacggag cgtaagccag tccgcgagcg
     3661 atcgctgact ggatcccgcc gcggatgcgg aagaatcctt ccgacgtccg ctcacccgtg
     3721 cagaatgcgc ggtcgcgcga atcgatgtcg ctggtcagca agtgagcgct gtcggcgtcg
     3781 gtgcgcgcca tgatcagcga aggcacgccc atcacgtcgg ccgccagccg cgccgcgacc
     3841 agcttctgaa ccgcttcggt ggttgggacg agaacttttc cgcccaggtg cccgcacttc
     3901 ttcgccgacg agagctgatc ctcgaagtga acgcacgccg cgcccgcttc gatcataccc
     3961 ttcatcagtt cgaaggcgtt gagcgtgccg ccgaagccgg cttcggcatc ggcgaccagc
     4021 ggagcaaacc acgtctcacc cggcgccact ttgccctcgg catggtgaac ctgatcggcg
     4081 cgctgcagcg cgttgttg
//
LOCUS       DASPUL010000324         5296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1425094, whole genome shotgun sequence.
ACCESSION   DASPUL010000324 DASPUL010000000
VERSION     DASPUL010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425094"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..4964
                     /locus_tag="VEJ00_07940"
     CDS             408..4964
                     /locus_tag="VEJ00_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01128.1"
                     /translation="MRAGVIILTCLSLVPIGLAQGARQSAPNAPIPDSDSDHVTERNE
                     WFFRGRLVPGKPSAELRRRAYQAKLQMRAQRAAALAAARSEALASVSSVPWAPLGPVP
                     LASDATGNGTQDYHQVSGRATAVAIDAADPTGNTVYIAGAQGGVWKSTNAANPSPSAV
                     TWTSLTDDQATLSFGSIAIQSGNTDPGKTLILAGTGEADNSGDSYFGLGILRSTDAGN
                     TWTLIPTANSGSLSFSGLGGTRMAFSSATPTTVVSAMATTSEGIISGAVTANTKRGLY
                     TSLDAGQTWTYNALLDPGSQATDATSATSVVYNAKAGMFFAAVRYHGFYSSPDGVTWT
                     RLASQPGGAALSTSACPPQSASNNQTCPIYRAEITVVPGRNEMYAWYIYFASTGRLTD
                     GGVWQSRNGGTSWTQIADTAISNCGDQFGCGVEQGPYNLELLAVANGSSGTDLYAGAI
                     NIYKCSINSQNPTCTSAPFMNLTHVYGCDPVGAPAHVHPDQHALAYVIPASGSDSGND
                     LMYFANDGGIYRALDGFGGLTSGSCSGANQFDDLNQNLGSMTQFVSFSQHPTDPNTLL
                     GGTQDNGSPATAQATTSANWGNVLGGDGGYNAIDPSTPLNFYASNPDIPPGGLGIQLC
                     SGGVNCNDGTFSFVATSASVGGDDGAFYFPYILDPQSSSALLVGTCRVWRGPRTGGVY
                     TALSPNFDTLGAGACSGNEVNLVRALAAAGPADNNGSEVVYAATSGLGPLDGPLASPV
                     GGRVWVTTNATAGASGFADVTANGPQGNINPDQYPVSSVAIDQSDSTGATAYVTIMGF
                     TGGSGHVWKTTNAGAAWTDFTANLPDSPANAVVIDATNKNVYVGTDVGVFVSSTSAAN
                     WAEVGPTPGQGVNGFLPNVAVTALAIFNSGGQELLRASTYGRGLWQWPIAATPNYQLS
                     ISNSPLTAFPNQVATFNGTATGVNGYASSVTLTCAAGTTAPPGTCTPTPSSVTPSNPA
                     TNAAFAVNVAGATGDFNFNIQGVGSDSQHVAHQIPVTLHVVDFTLTKPAPATVTVPRG
                     TSSALVNFQISAAGSFNQPVTVSCNPAIANAVCTLAPGTTVNPTSGNPVSMTASVAVP
                     AATAPGNYTVTLQATTAGAPAALTTDFTLKVTTNPDFVMTEPSPFPEVNVGSTGTNGP
                     ISIAAQDGFTGTVTLSCPPTFGANSCSVSPASVSSFPATATLTINGTSFLAGTYQIAV
                     TGVSGTISHSVGVPFDVGDYMISGTQAITVAPGGKGTANLTFTSSTFYSGKINASCDA
                     SALSGATCTLSPQNPLTVASGGTANLTVSVNVPNNASAGNYNININTQDTTGAPSGSF
                     TIVLTIAQDFVITSSTPSQTVNSGQATGPYNLTIRPVGASFNGAVALSCANLPSRAQC
                     VFNPSSPQTPGSSAVNVVMTITTGGTAANPSAMNQRSSRLALWLLSPGFMIGGVMIVW
                     SAASRQSGKRALLLPVSIAVLIGLLLTLASCSGVSSSGGGGGGGGGGSQSTPPGKYTI
                     TVTGSSSAIPADAGQSTQVTLVVN"
     gene            5066..>5296
                     /locus_tag="VEJ00_07945"
     CDS             5066..>5296
                     /locus_tag="VEJ00_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXX01129.1"
                     /translation="MTDSRYNVIVIGGGVVGLAVAFEITRRFPRTRLLVLEKEAGVAR
                     HQSGHNSGVIHSGIYYKPGSLKARLCVAGAAAM"
BASE COUNT          964 a         1915 c         1422 g          995 t
ORIGIN      
        1 cttcaccttc acgccggtct tgtgctcgta gctgtcggat atctcctgta gcgccgtgct
       61 caaatccgcc gcggccgcga ccacaatctc gcggctggtt ttctcctgtg ccccggccgc
      121 actcgaaatc accagcagcg ttgccgccag cccaacgcaa aagagcttca tgcctcttct
      181 ctcttcttca ccgcccggat gtcctgttct cgctccgccc ccgcacggaa ggccgattgt
      241 acaggcccag cgtcgcgcga tgcgcccgga tacgcgggga ggggtgtttc ccaaactggt
      301 ccgccatcct cccgccctgc cgcttttgaa ttgattggat tgacccgaat ttccatactg
      361 gatagtcgtc gcggactgcc atgctaacct gccatcctct ccgctcaatg cgagcaggcg
      421 ttattatcct cacttgtctc tccttggttc ccatcggact cgctcagggc gcccgtcaga
      481 gtgcccccaa tgcccccatc cccgattccg actccgatca cgttactgag cgcaacgagt
      541 ggttcttccg cggtcgcctc gtcccaggca agccttccgc cgagcttcgc cgtcgcgcct
      601 accaggccaa gctccagatg cgcgcccaga gagctgcggc tctcgccgcc gcgcgctccg
      661 aggcgctggc ctcggtgtcc tccgttccct gggcgcctct cggcccagtc cccttagcct
      721 ccgacgccac cggcaacggc acgcaagact atcaccaggt ttctggccgc gccaccgcgg
      781 tggccatcga tgccgcggat cccaccggca acaccgtcta catcgcagga gcacaaggcg
      841 gagtgtggaa gtcgaccaac gccgccaatc cttcgcccag cgccgtgacc tggacttcgc
      901 tcaccgacga ccaggccaca ctctccttcg gttcgatcgc catccagtcc ggcaacaccg
      961 atcccggcaa aacgctgatt ctggccggga ccggcgaggc cgacaactcc ggcgactcct
     1021 attttgggct gggaatcctg cgctctaccg acgccggcaa cacctggacc ctgattccca
     1081 ccgccaacag cggatcgctt tctttcagcg gcctgggcgg aacgcgcatg gccttcagca
     1141 gcgctacgcc caccacagtc gtatcagcga tggccacgac gtcagaaggc atcatctccg
     1201 gtgcggttac ggccaacaca aagcgcggtc tttacacgtc gctcgacgcc ggccagacct
     1261 ggacctacaa tgcgctgctc gatcccggca gccaggccac cgacgcgact tccgccacgt
     1321 ccgtcgtcta caacgccaaa gccggaatgt ttttcgcagc cgttcgctac cacggcttct
     1381 actcttcgcc tgacggtgtg acgtggactc gtctggctag ccaacccgga ggtgccgcgc
     1441 taagcaccag tgcctgccct ccacaatccg cgtcgaacaa ccaaacctgt cctatctatc
     1501 gcgccgagat cacggtcgtc ccgggccgca acgaaatgta cgcctggtat atttatttcg
     1561 cttcaacggg gcgcctgacc gacggcggcg tctggcagag ccggaacggc ggtacatcat
     1621 ggacccaaat tgctgacacc gcgatttcca actgcggcga tcaatttggc tgcggcgtcg
     1681 agcaaggccc ttacaatctc gaactgctcg ccgtagccaa tggttcgtcc ggcaccgacc
     1741 tctatgccgg agccatcaac atctacaagt gctccatcaa ctcgcagaat cccacctgca
     1801 cctctgcgcc gttcatgaac ctcacccacg tctacggatg cgaccccgtc ggggcgccgg
     1861 ctcacgtcca tcccgaccag catgcgcttg cttacgtgat cccggcctcc ggcagcgatt
     1921 cgggaaacga tctgatgtac ttcgccaatg acggcggcat ctaccgcgcc ctggacggat
     1981 tcggcggcct gacctcaggc tcttgctccg gcgccaatca gttcgatgac ctcaatcaga
     2041 atctgggatc gatgacgcag tttgtcagct tttcccagca tccgaccgat cccaacactc
     2101 tgctcggcgg cacgcaggat aacggttccc ctgccaccgc ccaagccacg accagtgcta
     2161 actggggcaa cgttctcggc ggggatggcg gctacaacgc gatcgatccc agtacgccac
     2221 tcaactttta cgcttcgaac cccgacatcc ctcccggcgg tctcggcatt cagctctgtt
     2281 ccggcggcgt taactgcaac gacgggacat tcagcttcgt cgccacgagc gcctcagttg
     2341 gcggcgatga cggcgccttc tatttcccct acatcctcga cccgcaatcc tcgagcgccc
     2401 tcctggtcgg cacttgccgc gtctggcgcg gcccgcgcac gggtggcgta tataccgcgc
     2461 tcagcccgaa cttcgatacg ctcggcgcag gggcttgttc aggaaacgaa gtgaacctgg
     2521 tgcgcgcgct cgctgccgca gggccggccg acaacaacgg ctctgaggtc gtctacgccg
     2581 ccaccagcgg gctcggcccg ctcgacggac ccctcgcatc gcccgtggga ggccgcgtct
     2641 gggtcactac gaacgccacg gcaggagcgt cgggttttgc ggatgtcact gccaatggcc
     2701 cgcagggaaa catcaaccct gaccagtatc ccgtttccag cgtcgcgatc gatcagtccg
     2761 actcgaccgg agccaccgcc tacgtcacga tcatgggctt caccggaggg tcaggtcacg
     2821 tctggaagac cacgaacgcc ggcgctgcct ggactgactt caccgccaac ttgcccgact
     2881 cccccgccaa cgcagtcgtc atcgatgcca cgaacaagaa cgtgtatgtc ggcaccgacg
     2941 tgggtgtatt tgtgagttcc acatctgcag cgaactgggc cgaagtcgga cccactcccg
     3001 gtcagggtgt gaatggattc ctgccgaatg tcgccgtcac ggcgctcgcc atcttcaatt
     3061 ctggcggcca ggaactgctg cgcgcctcca cctacgggcg cggactatgg cagtggccca
     3121 tcgccgcgac tcccaactac cagctatcca tctccaactc gcccctgacc gcttttccca
     3181 accaggtcgc gacgttcaac ggcaccgcca ctggcgtgaa tggctacgcc agctccgtca
     3241 ccctgacctg cgccgcggga acgaccgccc cgcccggcac gtgtaccccc accccgtcgt
     3301 cggtcacgcc gtcaaatcca gcgaccaacg ccgcgtttgc ggttaacgtt gcaggagcaa
     3361 ccggagactt caatttcaac atccagggag tcggttccga cagccagcat gtcgcacacc
     3421 agattccggt cacactgcac gtggtcgact tcactctcac caagcccgct cccgcgaccg
     3481 taactgtccc gcgaggaacg agctcagctc tcgtcaattt ccagatcagc gccgccggat
     3541 cgttcaatca gccggtgacg gtctcctgta atcccgcgat cgccaacgcc gtctgcacgc
     3601 tcgctccggg cacaacggtc aatccaacct cgggcaatcc cgtcagcatg acggccagtg
     3661 tcgccgttcc cgccgccact gccccgggta actacaccgt gactcttcag gcaacgactg
     3721 ctggagcacc ggctgcgctc acgaccgatt tcaccctgaa ggtcactacc aaccccgact
     3781 tcgtcatgac cgaaccctcg ccttttcccg aggtcaatgt cggcagcacc ggcaccaacg
     3841 gcccgatctc catcgctgcg caagacggct tcaccggcac cgtgaccctg agttgcccgc
     3901 ccaccttcgg cgccaacagt tgcagcgtca gccccgcatc agtcagttcc ttccccgcga
     3961 ccgcgacgct cacgatcaac ggaacaagtt tcctcgccgg aacctaccag attgcggtca
     4021 ccggagtttc cggcacgatc tcgcactcgg tcggcgttcc cttcgacgtc ggcgattaca
     4081 tgatttcagg aactcaagcc atcacggttg ctccaggggg taagggaaca gccaatctca
     4141 cgttcacgtc ttccacgttc tacagcggaa agatcaacgc ctcctgcgac gccagtgcgc
     4201 tgtccggcgc aacttgcacg ctctctccgc agaacccgct cacggtcgcc agcgggggta
     4261 cggccaatct caccgtcagc gtcaacgtcc ccaacaatgc ctccgccggg aactacaaca
     4321 tcaacatcaa tacgcaggac acgaccggcg caccgagcgg ctctttcacc atcgtcctca
     4381 ccatagctca ggattttgta attacatcat ccactcctag ccagaccgtg aactccggcc
     4441 aggccaccgg cccgtacaac ctcacgatcc ggccggtagg ggcatcgttc aacggtgcgg
     4501 tcgcgctttc gtgcgccaat ctgccctcca gggcgcaatg tgtgtttaat ccgtcgagtc
     4561 cgcaaactcc agggagctct gctgtgaacg tggtcatgac catcacaacc ggcgggactg
     4621 ccgccaatcc gtcggccatg aaccagcgct caagtcgcct cgcgctgtgg ctgctctcgc
     4681 ccggattcat gatcggcggg gtcatgatcg tttggagtgc tgccagccgc cagtcgggga
     4741 aacgcgcgct cctgctgccc gtctcgatcg cagtattgat cgggctgctg ttgacgctgg
     4801 catcctgcag cggggtcagc agcagcggtg gtggcggagg aggtgggggc ggtggaagcc
     4861 agagcacgcc gcccggcaag tacaccatca ctgtaaccgg ttcatcttcc gcgattccgg
     4921 ccgatgcggg gcagagcacc caggtgactc tcgtcgtaaa ctagcactgc tgagcttggc
     4981 gtcgggaagg gcacggcttc tgccatgcgg gcacgatccg tggagatttt cagactccgg
     5041 accgtctgcc gcttcccgcc acttcatgac cgactctcgc tacaacgtga ttgtcatcgg
     5101 cggaggcgtc gtcggcctcg ctgtcgcgtt cgaaatcacc cggcgttttc cgcgcacccg
     5161 gcttttggtg ctggagaaag aagccggcgt cgcccgccat caaagtggcc ataacagcgg
     5221 cgttattcac tccggcattt actacaagcc cggatcgctg aaggcgcgcc tctgcgtcgc
     5281 cggcgctgcc gccatg
//
LOCUS       DASPUL010000325         3892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_465629, whole genome shotgun sequence.
ACCESSION   DASPUL010000325 DASPUL010000000
VERSION     DASPUL010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465629"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VEJ00_07950"
     CDS             <1..317
                     /locus_tag="VEJ00_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01130.1"
                     /translation="RSLGAGDWKLAGALGAFSGPGVLVDLLLGSVFVAGAMALVLVIY
                     KGRLLQTLRNIGHILASLVTFRLPGTQVSLDNPDSLKVPYGVALALTALLYAGAYVWR
                     VR"
     gene            319..864
                     /locus_tag="VEJ00_07955"
     CDS             319..864
                     /locus_tag="VEJ00_07955"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HXX01131.1"
                     /translation="MSNVPRLNFRKLLRETGGAEIAEAAAVLPVMFMIVLGIFWFGQA
                     FRIYGTITRAAQDGARAAAAPYCMTCTSANTPAVNASNAVQAGLQAAHLDPSQITPLS
                     KWTPPTVVDCVTGSAVACTDKGGVCVQTNVLLVDSTSAEPVCGVSVSFEYPYQFWLPF
                     TTLNQQQLQLQAIARVKAEVP"
     gene            861..1472
                     /locus_tag="VEJ00_07960"
     CDS             861..1472
                     /locus_tag="VEJ00_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HXX01132.1"
                     /translation="MRQGPSLRIWTAFVLRDSRAAQLVEFAVSLPLLVVFVVGIFDFS
                     GAFTLKQKLTNVARDAARAAAADPITDLSSANPISINDAFWVVDNYLKSNRIDDCGVT
                     YGSGVPTRPTWTFSANGGSCVGTGLIITINRGYYFPATTTSQPPGVNCTPQSATGATA
                     VVATCVSIQYAYSWRFGRAASLLGGSAVLPASISATAVAMNED"
     gene            1482..2846
                     /locus_tag="VEJ00_07965"
     CDS             1482..2846
                     /locus_tag="VEJ00_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HXX01133.1"
                     /translation="MKPPAFSGKRAPLTAHHAERGVTMVLVALCMMAIIAMAALSIDV
                     VTLYLAREEAQRSADAAALAAARIISLSGITTTANTTNSTADWQQICGGNTSVASVTA
                     KAVAQQNSVANNTATVHVTYSVRGAPAGNADCSGLGASFAINPTVIVQVTNNNMPTLF
                     SRIWSNTANSVSATAVAEAFNPSDSGTIAPGGEDIPVIPRCVKPWIIPNEDPINAGAR
                     FVRRRSGTIVTPGIRINGVGAGVIGEAFTLTSACTSGALDCSNMNGHTPAAGAYVPAL
                     VTVNAYGFPTCADAGIYQEAIGGCDQTTAYECGTVNGGSVDLTINPSGSTGDTEIGTA
                     CLINQQVSGTGQDTLDVSAYPYQMKSGASNQIFKTSGQAITASNSVVSLPIYDNSVGN
                     LFGTEPSVTIVGFLQVFVDSVDATNGNLHVHVLNVSGCGNNASPTLSAPGNSPVPFHL
                     ITAH"
     gene            complement(2917..>3892)
                     /locus_tag="VEJ00_07970"
     CDS             complement(2917..>3892)
                     /locus_tag="VEJ00_07970"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HXX01134.1"
                     /translation="FPRILASRWLPATLCVLLLAGATSAAQVVPRPASVRAVKVLGSK
                     DAVEIEVEASESLIPQTRVLTGPDRFVVDFPNAVPGLGVHNQAVNRGEVKDVRVGLFQ
                     SQPPITRVVIDLKTAQSYQVFPNGRTVMIKVTSGALEASIDVDDTAREAPTRPGLVVT
                     NYTPGAQRVPSEATLRPGTVEVSFRNGLLGIRANKATLSEVLNAVQQRTGADIGMLAG
                     TDQEKVVVNLGPAPAPEVLAALLNGSRFNYLILSAANNPQQLERVILTPRGAMLMPLP
                     VQQNLDTAEDDPPRPAPVEPAVANVPPNMPPPNNAPEARAGENDASDQ"
BASE COUNT          698 a         1279 c         1165 g          750 t
ORIGIN      
        1 tgcgcagcct gggagcaggc gattggaagc tggcgggcgc tttgggagcg ttttccggcc
       61 cgggcgtgct cgtggacctg ctgctgggct cggttttcgt ggctggagcc atggccctgg
      121 tcctggtgat ttacaagggt cgattgctgc agacgctgcg caatatcggc catattctgg
      181 cctcgctggt gacttttcgc ttgccgggca cgcaggtttc gctcgataac ccggattcgc
      241 tgaaagtccc ttatggcgtg gccctggctt tgacggccct gctgtatgcg ggcgcgtacg
      301 tgtggagagt tcgctgagat gtctaatgtg ccgcggttga atttccgaaa gctgctccgc
      361 gagacgggcg gcgccgagat cgccgaggcc gccgcagtac tgcccgtgat gttcatgatc
      421 gttctgggca ttttctggtt cggtcaggcc ttccgcatct acggcaccat cacacgcgcg
      481 gcgcaagatg gcgcccgcgc ggccgcggct ccctattgca tgacctgcac ctccgcgaat
      541 acacccgcgg tgaacgcctc caatgctgtg caggccggat tgcaggcagc ccatctcgac
      601 cccagccaga tcaccccttt aagcaaatgg accccgccca cggtggtcga ctgtgtcacg
      661 ggtagcgccg tcgcctgcac cgacaaaggc ggcgtctgcg tgcagaccaa tgtactgctg
      721 gtggattcca cctcggccga gccagtgtgc ggcgtttccg tcagcttcga atacccctac
      781 cagttctggc ttccgtttac cacgctgaac cagcagcaac tgcagctgca agccatagcc
      841 cgcgtgaagg cggaggtgcc atgagacagg gccccagtct tcggatctgg accgcgttcg
      901 tgctgcgcga ttcccgggcg gcgcagctgg tggagttcgc cgtctctctg ccactgctgg
      961 tcgtgtttgt cgtggggatt ttcgatttca gcggcgcctt caccctcaag cagaaactga
     1021 ccaatgtggc ccgcgatgcg gcccgtgcgg ccgccgctga tcccatcacc gacctgtcgt
     1081 ccgccaaccc tatctcgatc aacgatgcct tctgggttgt cgacaactat ctgaagtcga
     1141 accggatcga tgattgcggt gttacctacg gaagcggcgt cccgacccgc cccacgtgga
     1201 ccttctcggc caacgggggc tcgtgcgtcg gcacgggcct gatcatcacc atcaaccgcg
     1261 gatactactt cccggcaacc accacctcgc aaccgcccgg cgtcaactgc actccgcaat
     1321 cggcaacagg cgcgaccgcg gtcgtcgcta cttgcgtcag cattcaatat gcctacagct
     1381 ggagatttgg ccgggccgcc agcctgctcg gggggtccgc cgtgctgccc gccagcatct
     1441 cggcaacagc cgtcgccatg aacgaggact gaggaacagg aatgaagccg ccagcatttt
     1501 ccggaaagcg cgcacccctc acggcgcacc acgccgaacg cggcgtcacc atggttctgg
     1561 tcgctctctg catgatggcc atcatcgcca tggcggcgct ttctatcgac gtagtcacgc
     1621 tgtatcttgc gcgcgaggaa gcgcagcgat cggccgatgc cgcagctctc gccgctgccc
     1681 gcatcatctc gctttccggt atcaccacca ctgcgaacac gaccaattcc accgccgact
     1741 ggcagcagat ctgcggcggc aataccagtg tggccagcgt tacggctaag gcggtagcgc
     1801 agcagaactc ggtcgccaat aacacggcta cggttcatgt cacttattcc gtccgcggag
     1861 cccctgccgg caatgctgac tgctccggcc tgggcgcatc gttcgccatc aatcccacgg
     1921 tgatcgtgca ggtcaccaac aacaacatgc ccacgctgtt ctctcgcatc tggagcaaca
     1981 cggccaactc ggtgagcgcc actgccgtag ccgaggcctt caacccgtcc gattccggca
     2041 cgatcgctcc gggcggcgaa gacatcccgg tcatcccgcg ctgcgtcaag ccgtggatca
     2101 ttcccaacga agatccgatc aatgccggcg cccgattcgt gaggcggaga agcgggacga
     2161 tcgtgacacc aggcatcagg atcaacggcg tcggcgccgg tgtaatcggc gaagctttca
     2221 ctttgacctc cgcctgcacg tccggggctc tggattgctc caacatgaat ggccatacgc
     2281 cggcggcggg cgcctacgtt cccgctctcg tcacggtaaa cgcatatgga ttccccacct
     2341 gcgctgacgc cgggatctac caggaggcta tcgggggctg cgaccagacc accgcctatg
     2401 aatgcggcac ggtcaacggc ggatcggtcg acctcacgat caatccgagc ggatcaacgg
     2461 gagacaccga aatcggaacc gcgtgcctga tcaatcaaca ggtcagcggg acagggcagg
     2521 acacacttga tgtgagcgcc tatccctatc agatgaagtc cggagccagc aatcagatct
     2581 tcaagaccag cggtcaggcc attactgcga gcaacagcgt cgtgagtttg cctatctacg
     2641 acaattccgt ggggaattta ttcggcacgg agccgtcggt tacgattgtg ggcttcctgc
     2701 aggtcttcgt cgacagcgtc gacgccacca acggcaacct gcatgtccat gttctcaatg
     2761 tgtcgggctg cggcaacaac gcctctccaa cgctttccgc ccctggaaac tccccggttc
     2821 ccttccacct gatcactgcg cattagtccg tgcagagaga tacactgctc gcgtgaaggg
     2881 aggcaccagc aaagagggat tttgcggccg ggattcctac tggtcggatg cgtcgttctc
     2941 gcccgctctg gcttcgggag cattattggg cgggggcatg ttgggcggga cgttggccac
     3001 cgccggctct acgggcgcag gccgaggggg gtcgtcctca gcggtgtcga ggttttgctg
     3061 cacgggcagc ggcatcagca tggcgccgcg cggagtcagg atcacccgct ccaactgctg
     3121 cggattgttg gccgcgctca gaatcagata gttgaaccgg gatccattca gcagcgccgc
     3181 cagcacttcc ggagccggcg ctggacccag gttgaccacg actttctcct ggtcggttcc
     3241 ggccagcatg ccaatgtcgg cgcccgtgcg ctgctgtacc gcgttcaaga cctccgacag
     3301 cgtcgccttg ttcgcccgga tcccgagcag cccgttgcga aatgaaacct ccaccgtgcc
     3361 cggcctgagt gtcgcttcgc tgggaactcg ctgcgctccg ggagtgtaat tcgtcacgac
     3421 aagcccgggg cgggttggcg cttcccttgc agtatcgtcg acgtcaatcg aagcttccag
     3481 cgcgccgctg gtcaccttga tcatcaccgt ccggccattg ggaaaaacct ggtacgattg
     3541 cgccgtcttc agatcaatca ccacgcgcgt gatcggcggc tgcgactgaa aaagccccac
     3601 ccgcacgtcc ttgacttcgc cgcggttcac cgcctgattg tgtacgccga ggccgggaac
     3661 agcgttgggg aaatcaacca cgaagcgatc cggcccggtg agtacacgcg tctgcggaat
     3721 cagactctcg ctggcctcga cttcgatctc gacggcgtct ttacttccca gcaccttcac
     3781 ggctcggacc gatgcgggac gaggcacgac ctgcgccgct gacgtcgctc ccgctagcag
     3841 gagaacgcag agcgtggccg gcagccaccg gctcgcgagg atgcggggaa ac
//
LOCUS       DASPUL010000326         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1837678, whole genome shotgun sequence.
ACCESSION   DASPUL010000326 DASPUL010000000
VERSION     DASPUL010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837678"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VEJ00_07975"
     CDS             complement(<1..239)
                     /locus_tag="VEJ00_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01135.1"
                     /translation="MQRIGSVLLAITVTLPLSAQVLAPPPPQSARQALIEMFFGTRED
                     DFAKHLPEAARQTLIRKGENPETSVALRISAIGRQV"
     gene            434..1414
                     /locus_tag="VEJ00_07980"
     CDS             434..1414
                     /locus_tag="VEJ00_07980"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HXX01136.1"
                     /translation="MKLLIVVQHRLDLWNVPKWFSDRLGQEFPQLQIVQRNHYAGIEQ
                     LLHDAEIIFTLSLRPEQFVLAQRLRWIHTPSAAVHQLMFPELVNSEVAVTNSGEVHGP
                     VVAEHVMALILALAKKIPQAVLLQQKRIWGQQEVWNAHPRELAGATLGLIGVGSIGRR
                     VARFASALGMRVIAVREHVEKGTPEGVEAIFAPLALDDFLRQCDYVVLAAPLTPSTVG
                     IINAVRLAAMKPDACLINVGRGPLVDTAALAEALRGYRIAGAALDVFEREPLPPDSPL
                     WGVENLLITPHTAGLTDKLWHRHYELFSGNLRRYLAGQPLQFVVDKRKGY"
     gene            1499..2851
                     /locus_tag="VEJ00_07985"
     CDS             1499..2851
                     /locus_tag="VEJ00_07985"
                     /inference="COORDINATES: protein motif:HMM:NF019314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXX01137.1"
                     /translation="MESRLILINLLVKLGVAAAVSSSLVRAKEFKALLFREDRTFRQK
                     VYLVLWFALPIMIGVWIRISQKSFVAADFSFETMLLLGVIGGRWAGSLGGVLVGLPAL
                     LHGEWAAMPFDVLCGFLAGQLRTLATDKDDIWSFSPFIDQSIFRLIRRNLPRPRLFDW
                     QVMFFWTVLGLRFVQTQLFRSFPSSIFSIESPGNWWVWGAIYAASVTAIGIELKIFNS
                     VRIQIKLEEQERLLLHARMEALQNQVNPHFLFNTLNSISSLVRFDPDMARDVIFKLAN
                     ILRRLLSSSEAFAPLREEFEFIDNYLDIEVVRFGRDKLHVVKELDPASLDVVVPSMLL
                     QPLVENSIKHGLSPKVEGGSIYLRSRVSDARLIIEVEDDGVGMGGAQLRESSHWSGMG
                     IGLANVSERLQVLYGDTARMTIDSREGKGTLIRLRLPILHTEGSIPAALYEERSSTRR
                     "
BASE COUNT          562 a          834 c          871 g          605 t
ORIGIN      
        1 acttggcggc cgatggcgga gatgcgcaag gccacggacg tctcgggatt ttctcctttg
       61 cggatcagag tctggcgggc agcctcggga agatgcttcg cgaagtcatc ctcccgcgtg
      121 ccaaaaaaca tctcaatcaa cgcctgccgg gccgattgcg gaggcggcgg ggccagaacc
      181 tgcgcggaaa gcggcaaagt gactgtgatt gccagaagaa cagatccgat tcgctgcata
      241 tgtcttctcc acgcgagcgg cggagccctc agtacccaat gtctgcgatc taagactccg
      301 ggcagcgcat caaggttcct gttctttcag gaacgtgtgg tatcgaatat cttgctggat
      361 ggttgccagc gaattaagga tgcctcctgc cgcctgcctc aatgttaaat tccgggatcc
      421 ggacttgcgc accatgaagc tgctgatcgt ggtccagcac cgccttgacc tctggaacgt
      481 gccgaagtgg tttagcgacc ggctggggca agagttcccg caactgcaga ttgtccagcg
      541 caatcattac gccgggatcg agcaactcct gcatgacgca gaaatcatct tcacgctctc
      601 cttgcggcct gaacaatttg tcctggcgca acgcttgcgc tggattcaca cgccgagcgc
      661 ggctgtgcac caattgatgt tccccgaact ggtgaacagc gaggtggcag tcacgaattc
      721 gggagaagta cacggaccgg tagttgccga acatgtgatg gcgctgatct tggcgctggc
      781 caagaagatt ccacaggctg tactgctgca gcagaagcgg atctgggggc agcaggaggt
      841 ctggaacgcg catccccgcg aactcgccgg cgcaactttg ggattgattg gcgtgggcag
      901 tatcgggaga cgcgtcgcca ggttcgcctc ggccctgggc atgcgtgtga tcgccgtccg
      961 ggagcacgtg gaaaagggaa ctcccgaagg tgtggaggcc atcttcgctc cattagcgct
     1021 ggacgacttc ctgcggcaat gcgactacgt cgtgctggcc gctcccctga cgccatcgac
     1081 ggtaggcatc atcaatgcag tccgtcttgc cgcaatgaaa cccgacgcct gcctgatcaa
     1141 tgtcgggcgt gggccactgg tggatacggc tgcgctggct gaggccctgc gcggatacag
     1201 gatcgccgga gccgccctgg atgtgtttga gcgcgaacct ttgccgccag attctccgct
     1261 gtggggcgtc gagaacctgc tgatcacgcc gcacaccgcg ggtctcacag acaagctctg
     1321 gcaccggcac tacgaactgt tctccggtaa tcttcgtcgt tacctggcag ggcagccact
     1381 gcagttcgtc gtcgacaagc gaaagggcta ttgaacacct gcgatcgagc ttccgccgac
     1441 gtgctacagt ttgatcattg gcggctagca cgggcaagtt caatcgaagt cagatcccat
     1501 ggaatcacgc cttatcctga tcaacctgct ggtcaagctg ggcgtcgcag cggcggtttc
     1561 cagcagcctg gtgcgcgcga aagagttcaa agccctgctg tttcgcgagg accgtacctt
     1621 ccggcagaag gtgtatctgg ttctgtggtt tgccctgccg atcatgatcg gcgtgtggat
     1681 ccggatctcg cagaaaagct ttgtcgctgc cgatttcagc ttcgagacga tgctcctttt
     1741 gggggtgatc ggggggcgct gggcgggatc gctcggcggc gtgctcgtgg ggcttcccgc
     1801 tttgctgcac ggcgaatggg ctgccatgcc tttcgacgtg ctttgcggtt tcctggccgg
     1861 acaattgcga acgctagcta ccgacaagga cgacatctgg tccttctcgc cattcatcga
     1921 tcagagcatc ttccggctga tccggcgcaa tctgccccgt ccccgtctgt tcgactggca
     1981 ggtcatgttt ttctggacgg tgttgggctt gcgcttcgtg caaacccaac tgttccgctc
     2041 ctttccgagt tccatcttca gcatcgagag cccaggtaac tggtgggtgt ggggagcaat
     2101 ctacgccgct tcggtaacgg cgatcggcat tgaattgaag atcttcaaca gcgtgcgtat
     2161 ccagatcaag ctggaggaac aggaacggtt gctgctccac gcccgcatgg aagccctgca
     2221 gaaccaggtg aacccgcact tcctgttcaa caccctgaat tccatttcgt cgctggtgcg
     2281 attcgatccc gacatggcgc gggacgtgat ctttaagttg gcgaacattc tgcgccgttt
     2341 gctcagcagc agcgaagcct ttgccccgct gcgggaagag ttcgaattca tcgacaacta
     2401 tcttgacatc gaggtggtac ggttcggacg cgacaagctt cacgtggtca aggaactcga
     2461 tcccgcttcg cttgacgtgg tcgttcccag catgctgctg cagccgctgg tggaaaattc
     2521 catcaagcac ggcctgtcgc cgaaagtgga aggcggaagc atctacctgc gcagccgggt
     2581 ctctgacgcc aggctgatta ttgaagtcga agacgacggc gtaggcatgg gaggagcgca
     2641 gctgagagaa agcagccact ggtcaggcat gggcataggc ctggccaatg tctcggaacg
     2701 tctacaggtg ctctacggcg acaccgcacg catgaccatc gacagccgcg aagggaaggg
     2761 aactctgatt cgcctgcgcc tgcccatcct gcacaccgaa ggctcgatcc cggccgcgct
     2821 ctacgaagag cgttccagca cgcggcgata gaactcctca tactgcggaa tg
//
LOCUS       DASPUL010000327         2612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1837820, whole genome shotgun sequence.
ACCESSION   DASPUL010000327 DASPUL010000000
VERSION     DASPUL010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1837820"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VEJ00_07990"
     CDS             complement(<1..494)
                     /locus_tag="VEJ00_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01138.1"
                     /translation="MKRAATVVVVLSAVLGLSTWSFAQGDKTTQNPPAGQAAGQAAAP
                     QGKRPPQAKTQPEFDAYKAAVGLADAAATEKAADDFAAKFPDSELRVVLYRSAMQKYQ
                     QVNNADKMLDMAHKALTFDADEPEALVGVAQVLAERTRDTDLDKDQRLAEAKKDAERA
                     LVTI"
     gene            complement(639..2150)
                     /locus_tag="VEJ00_07995"
     CDS             complement(639..2150)
                     /locus_tag="VEJ00_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01139.1"
                     /translation="MLPGSMLAVLLAAAAPVLAADSNNPITLDTSETLFTVLAAMNTC
                     GYEAGLNTSDAQRLNIRAEIQRTLKESDEAQAVVDTMCEWYQVHQSRDAVHDLSQYVS
                     LALYLQGPPNFLPRVREDDLPPDAAPIATFGKQLEQFYDKVHLHSIWERHRANYAALV
                     ARYHDPLSKMVFDTDIYLKMQSGGYLGRTFTIYLDFLGDPDAANARNYGTEYAVVVFP
                     SPDPNASEPLKMAQIRHTYLHYLLDPLADKHFSAVKRIEPLLQSVRSAPLEESFKRDI
                     SLLVTECLVRAVEIRTSGNKQTAEAMRVQAVDDAVKQGYVLTRYFYNALLAFEKDPAG
                     LRNDYGDILDAIDVKKEQKAASEVEFASTTAPELVQLSRLEDRRILVTAEKRLEAGDP
                     KGAQELAQQAIERKIGDQGRALFILAQVAVASKKRDEAADNFQKAIQATKDPKVVGWS
                     HVYLGRILDMKEDREAAINEYRAALTAGAGLPEVKAAAERGLQQAYEPPAKPQ"
     gene            complement(2357..>2612)
                     /locus_tag="VEJ00_08000"
     CDS             complement(2357..>2612)
                     /locus_tag="VEJ00_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX01140.1"
                     /translation="APQMELIPLWGLCDMYDRAGNPEKSQGCWHRATDLMAQQMGQNN
                     PQLAQSMTNEATALRKLGRTDEALKLEQRVTGLQSVTSKH"
BASE COUNT          434 a          875 c          722 g          581 t
ORIGIN      
        1 tcgatcgtaa ccaatgcccg ctctgcgtcc ttcttggcct cggccagccg ttggtccttg
       61 tcaagatccg tgtcccgcgt ccgctcggcc aaaacttgtg ctactccgac cagcgcctcc
      121 ggttcatcgg cgtcgaaggt cagtgctttg tgcgccatgt ccagcatttt gtcggcgttg
      181 ttgacttgct ggtacttctg catggccgac ctgtagagca cgacccgcaa ctcgctgtca
      241 gggaacttgg ctgcgaagtc gtcggcagcc ttttctgtcg cggctgcgtc ggcgaggccc
      301 accgctgcct tgtaggcgtc gaactccggc tgtgtttttg cctgtggcgg ccgcttgcct
      361 tgcggcgcag cagcctgtcc tgctgcctgc ccggccggcg ggttctgtgt cgtcttatcg
      421 ccctgtgcga aactccatgt gctcaatccc agcactgcac tcaagaccac aacgacagtc
      481 gctgctcgct tcatgcgctc tcctctgacc actgccctct ggttttccat cccgctgccc
      541 gaactctcga ccgtgcattc tatcgcaggg atcgggaaca tcaattgttt ctgattcaaa
      601 gccgctgact cccggagccg aggcgcgtcg ctccagcctc actgcggctt cgccggtggc
      661 tcgtaagcct gctgcagccc ccgctccgcg gctgctttga cctcgggtaa tcccgcgccg
      721 gccgtcaacg ccgcgcgata ttcgttgatc gcggcctctc ggtcttcttt catgtccaga
      781 atcctgccca ggtacacatg cgaccaaccc acaaccttcg gatccttcgt agcctgaatc
      841 gccttttgga agttgtccgc agcttcgtct ctctttttcg aggctaccgc cacctgcgcc
      901 aggatgaata gcgcccgtcc ctggtccccg atcttgcgct cgatcgcctg ctgcgccagt
      961 tcctgcgcgc ctttcggatc gcccgcctcg agccgcttct cggccgtcac caggatgcgc
     1021 cggtcttcca gccgcgacaa ctgcacgagc tcgggcgccg tcgtgctcgc gaactccacc
     1081 tcggaggcag ccttctgctc cttcttcacg tcgatcgcgt ccaggatgtc gccgtagtcg
     1141 ttgcgcaacc ctgccggatc tttttcgaag gccagaagcg cgttgtagaa gtaccgcgtc
     1201 aggacatacc cctgcttgac cgcgtcgtcg accgcctgca cccgcattgc ttccgccgtc
     1261 tgcttgtttc cgctcgtccg gatctcgacc gcccgcacca ggcactcggt aacgagcaag
     1321 gaaatgtccc gcttgaagct ctcctccagc ggtgccgatc tcaccgactg caggagcggc
     1381 tcgatccgct ttaccgcgct gaaatgcttg tcggccagcg gatcgagcaa ataatgcaga
     1441 tatgtatgcc ggatctgcgc catcttcagc ggttcgctcg cgttggggtc gggagaaggg
     1501 aagaccacca cggcgtattc cgtgccgtaa ttgcgcgcat tggcggcgtc aggatcgccc
     1561 aggaagtcga gatagatggt gaaggttcgt cccaggtatc cgcccgactg cattttcaga
     1621 taaatgtcgg tatcgaacac catcttcgac agcggatcgt gatagcgcgc caccagcgcc
     1681 gcatagtttg cccggtgccg ttcccagatc gagtgcagat gcaccttgtc ataaaactgc
     1741 tccagttgct tgccaaacgt cgcgatcggc gccgcatccg gtggcagatc gtcttctctc
     1801 actcgcggca gaaaattcgg tggcccctgc aaatacaagg ccagtgatac gtactgcgaa
     1861 aggtcgtgca ctgcatcccg gctctggtga acctgatacc actcgcacat cgtgtccaca
     1921 acggcctgcg cttcatcgga ctccttcagc gtcctttgaa tctcagcccg gatgttgagc
     1981 cgctgcgcat ctgacgtgtt cagtcccgcc tcgtacccgc atgtattcat cgccgccagc
     2041 acggtaaaca gcgtttcgct ggtgtccagc gtgatcggat tattgctgtc ggctgccagc
     2101 accggtgccg cggcagccag cagaaccgcc agcatcgaac caggaagcac aaacctcaga
     2161 aaacggctca ggaaaccctc ccgcgggatg cgacggccac gcacgcctgg ctggccgggg
     2221 aaagtacaag gcagaacact ctagagttta cgggctttta gagcaggggg tcaatgcggt
     2281 gttggggagt aggagataga ggcccgcgaa ggcgtactcc ctgccccgga ccccagaact
     2341 gggaatcatc gtcgccttag tgcttcgatg tcacactctg cagtccggtt acccgctgct
     2401 cgagcttcag cgcctcgtcg gttcttccca gcttgcgcag ggccgtcgct tcgttggtca
     2461 tcgactgcgc tagttgcggg ttgttctgcc ccatctgctg ggccatcaaa tcggtcgcgc
     2521 gatgccagca gccctgcgat ttctccggat tcccggcacg atcgtacatg tcgcacagtc
     2581 cccacagcgg gatcagctcc atctggggcg ct
//
LOCUS       DASPUL010000328         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_332686, whole genome shotgun sequence.
ACCESSION   DASPUL010000328 DASPUL010000000
VERSION     DASPUL010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332686"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..722
                     /locus_tag="VEJ00_08005"
     CDS             69..722
                     /locus_tag="VEJ00_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HXX01141.1"
                     /translation="MTLAIFLAIDYARTQKQMEKRALLQKAPVESVPMMVPAMVAGFE
                     VPENVRYHAGHTWALSESRELVRVGLDDFASKLIGKIDSITLPQRGRWVRQGQKIWTI
                     FRDGKSVDMVSPIEGTVTDVNQAVVNNPELARKEPYGDGWLLKVQAPDAKVNFRNLLG
                     GALARLWTEESALRLRKKMPIAMASALAQDGGVAVDDIAIHMPDQDWAELTKEFFLS"
     gene            complement(821..1384)
                     /locus_tag="VEJ00_08010"
     CDS             complement(821..1384)
                     /locus_tag="VEJ00_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01142.1"
                     /translation="MVKSLSQRSLRQFFATSAVQDFAFVICDPNHKMRCETSRHRSRQ
                     IGETRSLAMNEYAILSNRKRALIALAHSLVFLGIACHGFLSPRPAAVLVGPRATVNVL
                     LIAIYLTVAAILAWLVSISKCARERVYFALCASSATFGLLRLIFGDAALPVAQYLRVI
                     MLTSAVLVGGWIFRSFSRPAREDVQID"
     gene            1442..>2576
                     /locus_tag="VEJ00_08015"
     CDS             1442..>2576
                     /locus_tag="VEJ00_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXX01143.1"
                     /translation="MTRLRFGLASLMLLLLAGINGFAAGQTQPAQAHAIVLHAARLLD
                     VRNGRLIQPGEVLVEGPRIVEVGSTVKHPAGAEVIDLGDRTLLPGLIDAHIHLFLHPG
                     AEDLQTVEESVPQRTIMATLAARDDLMAGFTAERDMGTEGAGSADTAVRNAIDEGKIP
                     GPRLRVSGNAINILGGHEDAIGYNPAQHVLANADYANNADELVAVMREQFKEGADFIK
                     IYETGADSVHDGKFSTPYQYTEAQLEAAVKEAGRLGRRVAVHATGEPGTLFAAQAGVA
                     SVDHADQLSDETMRLMKEKQIFAVPTFTIFEYFAEHAATPAQSAREHQTLDLKIQEFR
                     KQIAAGVPMAVGSDVGPFPHGSQAREFVLMAKYGMSPLAVLQAD"
BASE COUNT          526 a          710 c          882 g          458 t
ORIGIN      
        1 ggtgcgagtg accccgaaga gatgaggcac tccgggcgag gtgggttatg tcagtcctac
       61 tggttctctt gacactagca atcttcctgg caattgacta cgcgcgcact caaaagcaga
      121 tggaaaagcg ggcactgtta cagaaagctc ctgtcgagtc ggtgccgatg atggttccgg
      181 cgatggtggc aggtttcgag gttcccgaga atgttcggta tcacgccggc cacacctggg
      241 cgctaagcga gagccgcgag ctcgtgcggg tgggcctgga cgacttcgcg tcgaagctga
      301 tcggcaagat cgacagcatt acgttgcccc aacgtgggcg ctgggtgcgg caaggccaga
      361 agatctggac gatcttccgc gacggcaagt cggtcgacat ggtttcgccg attgagggca
      421 cggtaaccga cgtcaatcaa gccgtagtga acaatccaga actggcgcgc aaagagccgt
      481 acggcgacgg ctggctgctg aaggtgcagg ctccggacgc gaaggtcaac ttccgcaacc
      541 tgctgggcgg agcgctggct cggctgtgga cggaagagtc ggcgctgcgg ttgcgcaaga
      601 agatgccgat cgccatggcc tcggcactgg cgcaggatgg cggcgtagcg gttgacgaca
      661 tcgcgatcca tatgccggac caggattggg cggagctgac gaaagagttc ttcctgtctt
      721 agcttgccgt tgcatgggcc tcgcctgaag cctccggttg gccgcccggg attcgtcccg
      781 ggcggctcac tttttgtggc tatttatcca acgaggggcg tcagtcgatt tgcacgtctt
      841 cacgtgcggg gcgggaaaaa gatcggaaga tccacccacc gaccaggacc gcgctagtca
      901 gcatgatcac gcgcagatat tgggcaacgg ggagggcggc gtctccgaag atgaggcgca
      961 gcaagccgaa ggtggcgctg ctggcgcaca gcgcgaagta gacgcgctcc cgggcgcact
     1021 tcgaaatgct caccagccag gcaaggatcg ccgcgaccgt caggtagatc gcaatgagaa
     1081 ggacattcac cgtcgcgcgc gggccgacca gcactgcggc gggcctgggc gacaggaagc
     1141 cgtggcacgc gatcccaagg aacacgagcg agtgagccag cgcgatcagg gcgcgcttgc
     1201 ggttgctgag gatggcgtac tcgttcatag ccaatgatcg agtttcgccg atttgccggg
     1261 aacggtgtcg tgatgtttcg caccgcattt tgtgattggg atcacagatg acaaaggcaa
     1321 agtcttggac cgcagaggtc gcgaagaact gccgcaaaga acgctgagaa aggctcttga
     1381 ccacgcttcg tcgcgctccc gtggtaggag accgaggcgt agtggtacca tcgcgggcgt
     1441 tatgactcgg ctacgctttg gactcgcttc cctcatgctt ctgttactgg ccggaatcaa
     1501 tggctttgcc gcgggacaga ctcaacctgc gcaggcgcac gcgatcgtcc tgcacgcggc
     1561 gcggctgctg gacgtgcgca acggtcggct cattcagccg ggagaagtcc tggtcgaagg
     1621 cccgcgcatt gtggaagttg gaagcacggt gaaacatccg gcaggagcgg aagtgatcga
     1681 tctcggcgac cgcacgctgt tgcccgggct gatcgatgcg cacattcatc tgtttctgca
     1741 tccgggtgcc gaggatctgc agacggtcga ggaatcggtg ccgcagcgca cgatcatggc
     1801 gaccttggcc gcacgcgacg acctgatggc cggcttcact gccgagcgcg atatggggac
     1861 ggagggcgcg ggttcagcgg acacggccgt gcgtaatgcg attgatgagg gaaagattcc
     1921 cggaccgcgg ctgcgcgtca gcgggaatgc catcaacatt ctcggcgggc acgaagatgc
     1981 gatcggctac aacccggcgc agcacgtcct cgcgaatgcc gactacgcca ataatgcgga
     2041 tgaacttgtg gcggtgatgc gcgagcaatt caaggaaggc gccgacttca tcaagatcta
     2101 tgaaacgggc gcggactcgg ttcacgatgg aaagttctca acgccctacc agtacaccga
     2161 ggcgcagctg gaagcggcgg tgaaagaagc agggcggctg ggccggcgcg tagcggtgca
     2221 cgctacgggc gagccgggaa ctctttttgc ggcgcaggcg ggcgtggcat cggttgatca
     2281 cgccgatcag ctcagtgacg agaccatgcg gctgatgaaa gagaagcaga tctttgccgt
     2341 gccgacattc acgatcttcg agtatttcgc ggagcatgcg gccacgccgg cgcagagcgc
     2401 acgggaacat cagacgctcg atctgaaaat ccaggaattc aggaaacaga ttgcggcggg
     2461 agtgccgatg gcggtgggat cggacgtagg accgtttcct cacggctcgc aggcgcggga
     2521 gttcgtgctg atggcgaagt atggaatgtc gccgcttgcg gtgctgcagg ccgatc
//
LOCUS       DASPUL010000329         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1976748, whole genome shotgun sequence.
ACCESSION   DASPUL010000329 DASPUL010000000
VERSION     DASPUL010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1976748"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VEJ00_08020"
     CDS             complement(<1..200)
                     /locus_tag="VEJ00_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01144.1"
                     /translation="MIQTLFGSLEEEKKTGFFDRMKQAVTRTRETLAERIEEVVSIGK
                     EIDRNTLDDLEATLIGADLGTVT"
     gene            complement(433..2274)
                     /locus_tag="VEJ00_08025"
     CDS             complement(433..2274)
                     /locus_tag="VEJ00_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXX01145.1"
                     /translation="MSSSTSNHARIPILYVILGVLLVISIVPMYLYSTWVEAINRDRL
                     KTNERMLQNTVTRSLADDLSQHERSLRVELNNLSYAIQVASGGNIAGQNIQAPELRAL
                     LENFVSSSDEIAYATLLDSDAKGISAPPIAPDAFLQRELERAFAAARDGRAYNGQALV
                     VGEGKSARTVFVVSTPVKYGPRFLGVIASVVDLQFLIRRLREVSGGGLTPYVVDAEGR
                     LVAAATPEFATGQDMKNLEIVRNFVDGGSKTHLGAATNEFTIKNGSERVEMLGTYSPV
                     MSLDWAVVVQKPQQEAYSSIIEMRHKSRLLVLVAALIIMLVSIYAARRITNPLSVLTQ
                     SSRALAKGDFSQRVQLKSSTEIGELAQTFNSMSEELEHFVEDLKRAAEENRALFMGSI
                     QMLAGAVDEKDPYTRGHSDRVTRYSMLIAKEMNLPATFMETLHVSAQLHDVGKIGIED
                     HILKKPGALTAEEFEVMKTHTTKGANILRPVTQLAEMLPGIELHHEALDGRGYPYGLE
                     GDQIPLLARVIAVADTFDALTTNRPYQQAHTPDQALQIIQNLAGKRLDPDAVKALLAV
                     YARGDIKIQRFTIMRPIAPPPVAAPVDTAASPAALAAETAALERTRV"
     gene            complement(2552..3559)
                     /locus_tag="VEJ00_08030"
     CDS             complement(2552..3559)
                     /locus_tag="VEJ00_08030"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HXX01146.1"
                     /translation="MSQIAVIGAGAWGTGIAIVLGRKGTHRVRLWAHETEVCDSINTR
                     RVNETFLPGRHIPESVTAGNDLASVLDGAQIVVSVMPSQHCRGLFERMRPWLQAHMLV
                     VSATKGLEESSLQRMTEVIAQVLQRNGGAAIGALSGPSFAQEVARGDPTAITIASQDP
                     ALLRTVQQEFSDPSFRVYTNSDVVGVELGGALKNIVAIAAGICDGLGLGHNSAAALIT
                     RGLAEMTRLVVACGGRAETMAGLAGLGDLVLTCTGGLSRNRSVGVELGRGRKLPEILA
                     GMHGMVAEGIFTTTAAVGLARARSVEMPITEQMHAILHQGKSPREAIHELMTRSGKSE
                     L"
     gene            complement(3564..>3884)
                     /locus_tag="VEJ00_08035"
     CDS             complement(3564..>3884)
                     /locus_tag="VEJ00_08035"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HXX01147.1"
                     /translation="VATGLGSFLMIAPTAVLIAAGIFLVVVLVFRYVSLGSVVAVSLF
                     PSLVWQLHQYGNTWPALAMMSLTALLIVLKHSSNISRLLAGTENRLGSKRAHNDNVKT
                     TRSG"
BASE COUNT          736 a         1125 c         1260 g          763 t
ORIGIN      
        1 gtaactgtgc cgaggtcggc accgatgagc gtagcttcga gatcatcgag ggtgttgcgg
       61 tcgatttcct tgccgatgga gacgacctct tcaatgcgct cggcgagggt ttcgcgcgtg
      121 cgcgtcacag cctgcttcat gcgatcgaag aagccggtct tcttttcttc ttccaggctg
      181 ccgaacaggg tttggatcat ggggtgccca aaccctgatt atacgggtgg ccaggacttg
      241 gctcgggcgc tcacgcacgg ttgaggtccg tcatttgccc tggggaaagc ttgaaaccgc
      301 gaagtgcgca aaggaactgc cgcaaaggcc gctaagaccg gcgtctggcc gcggaattcg
      361 gggaaggcag cgaactgcag ttgactcaat gatccgattg ctcaatgact caatcgcgca
      421 atggctcaat gcttagacgc gggttcgttc cagggctgcg gtttctgccg cgagtgcggc
      481 gggagaggca gcggtgtcca ctggcgctgc aaccggaggc ggtgcgatgg gccgcatgat
      541 ggtgaagcgc tgaatcttga tgtcgccgcg ggcgtacaca gcgagcagag ccttgaccgc
      601 gtcaggatcc aggcgcttgc cggcgaggtt ctggatgatc tgcagcgcct ggtcgggcgt
      661 gtgtgcctgc tggtaggggc gattagtggt cagggcgtcg aaagtgtcgg ctacggcgat
      721 cacacgggcc agcagaggaa tctggtcgcc ctccaagccg taaggatagc cgcggccatc
      781 gagggcttca tggtgcaact caatgccggg gagcatctcg gccagttgtg taaccgggcg
      841 caggatgttg gcccccttgg tggtgtgcgt cttcatgact tcgaactctt cagcggtgag
      901 cgccccgggc tttttcaaaa tatgatcttc gatgccgatc ttgcccacgt catgcagctg
      961 ggccgaaacg tggagcgtct ccatgaaagt agcgggcagg ttcatctctt tcgcgatgag
     1021 catcgaatag cgcgtgacgc ggtcggagtg gccgcgggtg tagggatcct tttcgtcgac
     1081 cgcgccggcc agcatctgga tggaacccat gaacagggcg cgattttctt cggcggcgcg
     1141 cttcaggtct tcgacgaagt gttcgagctc ctccgacatc gagttgaagg tctgggccag
     1201 ttcgccgatc tccgtgctgc tcttcagctg aacgcgctgg gagaaatctc ccttggcgag
     1261 ggcgcggctc gactgggtca gcaccgacag aggattggtg atgcggcgcg cggcataaat
     1321 gctcaccagc atgatgatca acgccgctac aagaacgagc aggcgcgact tgtgtcgcat
     1381 ttcgatgatg ctgctatagg cctcctgttg cggcttctgc accaccacgg cccagtcgag
     1441 ggacatgacc gggctgtaag tgcccagcat ttccacacgc tcggaaccgt tcttgatggt
     1501 gaactcgttg gtggccgcgc ccaggtgtgt tttgctgccg ccatccacga agttgcggac
     1561 gatttccagg ttcttcatgt cctggccggt ggcgaactcg ggtgtggccg cggcgaccag
     1621 acggccctcg gcgtcgacca cgtagggtgt caggcctccg ccgctgactt cgcgcaagcg
     1681 gcggatcagg aattgcagat ctacgacgga ggcgatcacg cccagaaatc tcggtccgta
     1741 cttgaccggt gtgctgacca cgaaaacggt gcgagcagac ttgccctcgc ccactaccag
     1801 ggcttgcccg ttataagcgc gcccgtcacg agcagcggca aaggcgcgct ccagttcgcg
     1861 ctgcagaaag gcgtccggcg caatgggggg agcggaaatc cccttcgcgt cggagtccag
     1921 cagggtggca taggcaattt catcggagga ggaaacgaaa ttctcgagca gcgcccgcaa
     1981 ctccggagcc tggatatttt gacctgcgat attgccgccg ctggcgacct gaatggcgta
     2041 ggacaggttg ttcaactcca cgcgcaacga ccgttcgtgc tgggaaagat cgtcggccag
     2101 ggaacgggtc accgtattct gcagcatgcg ctcgttggtc ttcaggcgat cgcgattgat
     2161 cgcttccacc catgtcgagt agagatacat gggcacgatg ctgatgacca ggagcacacc
     2221 gagaattaca tacagaatcg ggatgcgcgc gtggttggaa gtgcttgagg acaaggttct
     2281 cccttcactt cattcgcagg cgccgggacg aatggcggcg gttctcaagg tatccctaga
     2341 attttaatgt ggaatggccg gcgggagccg aaaaggcggg tgctgcggcg ttacggaagt
     2401 acccgtcaga tgtgcctttt ggtgcgggtt tggcaaggaa agtcaggagc tatgagctac
     2461 gagtcatgaa ctctgccaag gaactctccg cctcgcccgg aatgagaatg aagataggca
     2521 ctaaactcag gactcgcggt tcacgactcg ttcacagttc gctttttccg ctgcgcgtca
     2581 tcagttcgtg aatggcttcg cggggagatt tgccctggtg caggatggca tgcatctgct
     2641 cggtgatggg catttccaca ctgcgggcgc gggccaggcc gacggcggcg gtcgtggtga
     2701 agattccctc tgcgaccatg ccgtgcatgc cggcaaggat ttccggcagc ttgcgtcccc
     2761 gaccgagttc gacgccgacg ctgcgattgc gcgacaggcc tcccgtgcag gtgaggacca
     2821 gatcgcccag gcccgcgagt cccgccatgg tttcggcgcg gccgccgcag gccacgacca
     2881 gacgcgtcat ttccgccagg ccgcgtgtga tcagcgccgc cgccgaattg tggccgaggc
     2941 cgagtccgtc gcagatgcca gcggcgatgg caacgatgtt cttcaatgcg ccgcccagct
     3001 cgactccgac gacgtcgctg ttggtgtaga cgcggaagct gggatcgctg aactcctgct
     3061 gcacggtgcg cagcagcgcg ggatcttgcg aagcgatcgt gatcgccgtg gggtcgccgc
     3121 gggccacttc ctgcgcaaac gacgggccgc tgagcgcccc gatcgcggca ccaccgttac
     3181 gttgcagcac ttgcgcgatc acttcggtca tgcgctgcag agaactctcc tccaggccct
     3241 tggtcgcgct gacgaccagc atgtgcgctt gtaaccatgg ccgcatccgc tcgaacaagc
     3301 cgcggcagtg ttgcgagggc atcacgctga ccacgatttg agcaccgtcg agtaccgacg
     3361 ccaggtcgtt gccggcggtc acagattcgg gaatatggcg cccgggaagg aatgtctcat
     3421 tgaccctgcg cgtgttgatc gaatcgcaga cctcggtctc atgcgcccac aagcgcaccc
     3481 ggtgcgtgcc cttgcgcccc agcacgatgg cgatgcccgt gccccaggcg ccggcgccga
     3541 taaccgcgat ctggctcatg atcttatccg gacctcgtgg ttttaacgtt gtcattgtgc
     3601 gcgcgcttcg atcccagacg gttttcggtt ccggccagga ggcgggaaat attcgaagag
     3661 tgtttcagca cgatgagcaa ggcagtcagc gacatcatag ccaatgccgg ccacgtgttg
     3721 ccgtactgat ggagctgcca gaccagagaa gggaacagag agaccgcgac aaccgagccc
     3781 agcgatacat agcggaacac gagcacgacg acgagaaaga tgcctgccgc gatcaagact
     3841 gctgtcggag cgatcatcag gaacgagccc aagccggtgg cgac
//
LOCUS       DASPUL010000330         5335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_204555, whole genome shotgun sequence.
ACCESSION   DASPUL010000330 DASPUL010000000
VERSION     DASPUL010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204555"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            688..1467
                     /locus_tag="VEJ00_08040"
     CDS             688..1467
                     /locus_tag="VEJ00_08040"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXX01148.1"
                     /translation="MSKALLYDSTMCIGCKQCEQACADQNKLPYNDDIAREERQSDHK
                     YTVVMTKGDKFMRRLCMHCERPACASACPVGAFHKTKEGPVVYDVWKCIGCRYCMAAC
                     AFSQPKYEWGSLNPRVRKCIMCPDRIAAGKQTACAEICPTGATKFGERDDLIKEAQDR
                     IRQNPSTYLNRIYGLHEVGGTCVLILSGMKVEEFGYPASAKIGDTPVPEYTARVMDRV
                     PDFIPVWSLVLGGIYWISHRRQEVAEAEAAERKAKKNGGAR"
     gene            1464..2648
                     /gene="hybB"
                     /locus_tag="VEJ00_08045"
     CDS             1464..2648
                     /gene="hybB"
                     /locus_tag="VEJ00_08045"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF008133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HXX01149.1"
                     /translation="MRFKLTFWKAVFVVIMGLGLYSAFIRYTRGLGAATNMSDQFPWG
                     FWIGFDCLCGVMLAAGGFCIVGAVYLFNVTSLRSVMRPAILTAFLGYLLFIVGLLFDL
                     GRPWFIWHQLIYMNPHSVMFEVGMCVMTYTTVLFFEFLPNVFERFKLKTPIRWIHRIY
                     PVLIVLGILLSTLHQSSLGSLYLIMPSKLHPFWYSPALPFFFFASAVAVGLAMTIFES
                     TQSAKALGRHLELPVLVQLGGALLVALWVNALLRFEDYLRRGMLREIVKPSYEAYFLW
                     LELGLSFVIPIILLSFKRVRLSPNGLYLASVISLFGFITNRLNVSLIGFETYVGHHYM
                     PKWTEFSVTLMIIASGFALFGLAVKYLPIFPREPVVARESLAEPKLPLPAASPVFEHA
                     GD"
     gene            2635..4368
                     /locus_tag="VEJ00_08050"
     CDS             2635..4368
                     /locus_tag="VEJ00_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXX01150.1"
                     /translation="MPETDPTREPWLRLRPTLSAKLNVLLLAAMVLIFALLGYLNVRL
                     HRQHLEQNTLLSAERVSDVIRHSTTDYMLRNDRDGLYHSMQTMAGEPGVVKIRVFDQD
                     GKITYTTDPAELDHVVDKKAEACYACHAQSQPLAKLNRPDRFRIYRNGGGTRVLGIIT
                     PIENQPACSNAACHAHPPEQQILGVLDTNLSLAKADVQLAESSQRMILYTGCALLLIY
                     LLSWFFIWRLVGKPLKALKSGTERLAAGDLGYQIAVLSGDEIGELAHSFNDMSVQLQA
                     EHKQNLAWTHTLEERVELKTRELRRAHEHALHTEKMASIGKMAAVLAHEINNPLSGIL
                     TYAKLLRKWIDREELDRQKASGEGVRRYQEICESLDLIAAESRRCGDLVKNLLAFSRT
                     TPMNVQATDLNEIVRRSLRLVQHQLDLASIQVQLQLDPALPRVQCDAAQIEQVLLALV
                     MNALDAMPQGGNLWVRTSASRENGQVRIVVRDDGSGIPPEILPRLFEPFLTTKETGRG
                     VGLGLAISRSILERHSGTIEVQSEAGKGTTFTVTLPWDADADVGELARPATDKTSTPS
                     ATETATTASGR"
     gene            4374..>5335
                     /locus_tag="VEJ00_08055"
     CDS             4374..>5335
                     /locus_tag="VEJ00_08055"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXX01151.1"
                     /translation="MEAQGKILIVDDELSVRDSLGKWFREEGYEIGTAESASDALTRM
                     AENKWDLALVDIKMRGTDGIELQRRLHELDPELIVIIMTGYASVETAVTALKNGAYDY
                     VSKPLDPDEIAHLVKKALAHRRAEKENVRLRETVAEATRPGELVGQSPAMRKVFEAIE
                     TVGPTDANVLITGESGTGKELVVRAIHAASLRRFHPLVVIHCGALTETLLESELFGHE
                     KGAFTGAQYRKKGKFEIAEGGTVFLDEIGDISLKTQTDLLRVLQEHEITRVGGNQVIK
                     VDFRCVAATNKNLEMMIEEGAFRPDLFYRLNVFHIELPPLRDRR"
BASE COUNT         1120 a         1688 c         1495 g         1032 t
ORIGIN      
        1 gcttttgagt gcgcgcgtag tcaattgcca ggaagattgc tagtgtcaag agaaccagta
       61 ggactgacat aacccacctc gcccggagtg cctcatctct tcggggtcac tcgcaccctc
      121 cctaaaggca atctccggac caaaaccagg tgcagcccgg atgcatcccc cttgcatgcg
      181 gatctcttgc gatgtgaatg acataggaag tcgcaccggc cagcccatct gtatctgcag
      241 aaaaacacag cagccggttt cgatgaagat cgcaacaata gaattagcca ggaaagcacg
      301 cggaatcggc gatcgtgtcc caaacggcct accatcaata gtttgccgct gctcctcgct
      361 tgcccgaggg tgatgcgatc ttcatcaggc gtctgttgcg aaagacaacg ccattgagcg
      421 gacttcccgg ccgttgactg ggatcacccg caccggtgat gcgtatcacc gacgagaacc
      481 cccttcattg ttgaaactgt taactgtctg aagctgctga atttcgcgac agcctccgat
      541 gtgatgaggt aagcagcttg ttactgcgac catctcgctc cggttacacc ggtaacggca
      601 tcttcctgtc ttcatttcaa tttcatccag ctgtcgaatt ccggaccgct ccgcgagcac
      661 gaagctgcgt tcggggagaa atccttcatg agcaaagcac ttttatacga ttccacgatg
      721 tgcatcggct gcaagcagtg tgaacaggcg tgcgccgatc agaacaagct tccctataac
      781 gatgacattg ccagggaaga gcgccaatcc gatcacaaat acacggtcgt catgaccaaa
      841 ggcgacaagt tcatgcggag gctctgcatg cactgtgagc gcccggcgtg cgcttcggct
      901 tgcccggtgg gagccttcca caaaacgaaa gagggaccgg tcgtttacga cgtgtggaaa
      961 tgcatcggct gccgttactg catggccgcc tgcgccttca gccagcccaa gtacgaatgg
     1021 ggatcgctga atccgcgggt tcgcaaatgc atcatgtgcc cggaccgcat cgctgccggc
     1081 aaacagacgg cgtgcgccga gatctgcccc accggcgcca ccaagttcgg cgagcgcgac
     1141 gacctgatca aggaagcgca agaccgcatc cggcagaacc cgtcgaccta cctgaaccgc
     1201 atctacggac ttcatgaagt gggaggaacc tgcgtcctca ttctctccgg catgaaggtg
     1261 gaagaattcg ggtatcccgc atcggcaaag atcggcgaca cgccggtgcc cgaatacacc
     1321 gcccgcgtaa tggacagggt tccggacttc atccccgtct ggtcgctggt gctgggcggg
     1381 atctactgga tttcccatcg ccgccaggag gtggcagaag ctgaagccgc cgaacgaaag
     1441 gcaaagaaga acgggggcgc gcgatgagat tcaagctcac tttctggaaa gccgtattcg
     1501 tcgtcatcat ggggctcggc ctctattccg ccttcatccg ttacacgcgc ggcctgggcg
     1561 cggctaccaa catgagcgac cagttcccct ggggcttctg gatcggcttc gactgcctat
     1621 gcggggtcat gctggccgcg ggcggcttct gcatcgtcgg cgcggtctac cttttcaacg
     1681 tcacaagcct gcgcagcgtc atgcgtcctg caatattgac tgcgtttctc ggctacttgc
     1741 tcttcatcgt cggactgctc ttcgatcttg gccgcccctg gttcatctgg caccagctca
     1801 tctatatgaa tccgcactcg gtcatgtttg aggtgggcat gtgcgtgatg acctatacga
     1861 cggtgctctt cttcgaattc ctgcccaatg tgttcgagcg cttcaagcta aagacgccca
     1921 tccgatggat tcacaggatc tatcccgttc tgatcgtgtt gggcatcctg ctctcgacct
     1981 tgcatcagag ttcgctcgga tcgctgtacc tgattatgcc gagcaagctc catcccttct
     2041 ggtactcgcc ggccctgccg ttcttcttct tcgcctcggc ggtggcagtc ggtctggcaa
     2101 tgacgatctt cgagtccacg caaagcgcga aggccctggg acgccatctc gaacttccag
     2161 ttctggtcca acttggcgga gccttgctgg tcgcgctctg ggtgaatgcc ctgcttcgct
     2221 tcgaagacta ccttcgccgc ggcatgctgc gtgaaatcgt gaagcccagc tacgaagcgt
     2281 acttcctgtg gctcgagctt ggcctgtcgt tcgtgatccc gattattctg ctgagcttca
     2341 agcgagtcag gctctctccc aacgggctgt accttgcttc cgtcatcagc ctgtttggct
     2401 tcatcaccaa tcgcctcaac gtcagcctca tcggattcga gacctacgtc gggcatcact
     2461 acatgccgaa gtggaccgag ttttcggtga ccctcatgat catcgccagc ggcttcgctc
     2521 tgttcggatt agcggtgaaa tatctgccca tcttcccccg cgagccagtc gtcgctcggg
     2581 aatcgctggc cgaaccgaaa cttccgctgc ccgcggcttc gccggtattc gagcatgccg
     2641 gagactgatc caactcgcga accgtggctg cggctgaggc ccacgctaag cgccaagctg
     2701 aacgtactcc tgctcgccgc gatggtgctc atcttcgccc tgctgggcta tctcaacgtc
     2761 cgcctgcacc ggcagcatct ggagcagaac acgttgctca gcgccgagcg cgtcagcgac
     2821 gtgatcaggc acagcaccac cgactacatg ctgcgcaacg accgcgatgg actctaccac
     2881 tccatgcaaa ccatggcggg agagccgggc gtggtcaaga ttcgggtgtt cgaccaggac
     2941 ggaaagatta cctacaccac cgatcccgcc gaactcgacc acgtcgtcga caaaaaagct
     3001 gaggcttgtt acgcctgcca cgcccaatcc cagcccctgg cgaagctgaa ccggcctgac
     3061 cgcttccgga tttatcgcaa cggcggaggc acgcgcgtcc ttggcatcat cactcccatc
     3121 gaaaaccagc cggcgtgctc caatgccgcg tgccacgccc atccccctga acagcagatt
     3181 ctcggtgtgc tcgacaccaa cctctcgctc gccaaggccg acgtacagct tgccgaaagc
     3241 agccagcgca tgatccttta caccggctgt gcgcttcttt tgatctacct cttgagctgg
     3301 ttctttatct ggcgcctggt cggcaagccc ctcaaagcgc tgaagagcgg cacggaacgc
     3361 ctggctgccg gcgatctggg ctatcagatc gcggtgctgt cgggagatga gatcggcgaa
     3421 ctcgcacatt ccttcaacga tatgagcgtg cagctgcagg cggagcacaa gcagaatctc
     3481 gcctggactc acacactcga agaacgggtc gagctcaaaa cgcgcgaact caggcgcgcc
     3541 cacgagcatg cgctgcacac cgagaagatg gcatccatcg gcaagatggc cgccgtgctc
     3601 gctcatgaga tcaataatcc gctttccggc atcctgactt acgcgaagct gctgaggaag
     3661 tggattgacc gcgaagaact ggaccggcag aaggcttccg gagaaggagt gaggcgctac
     3721 caggagattt gcgagtccct cgatctcatc gccgccgaga gccgtcgctg cggcgacctg
     3781 gtcaaaaacc ttctcgcgtt ctcgcgcacc actcccatga acgtgcaggc aacggacctc
     3841 aacgagatcg tccgccggtc cctgcgcctg gtgcagcacc aactcgatct ggcaagcatt
     3901 caggttcagc tgcaactcga cccggccctg ccgcgcgtcc aatgtgacgc cgcgcagatc
     3961 gagcaggtgc tgctcgccct ggtaatgaac gctctggacg ccatgccgca aggcggcaat
     4021 ctatgggtca ggaccagcgc cagtcgcgag aacggccagg tccgcatcgt ggtgcgcgac
     4081 gacggcagcg gcattccccc cgaaatcctg ccccgtctct tcgagccgtt ccttaccacc
     4141 aaggaaaccg gccgcggggt cggcctgggc ctggccatca gccgcagcat tctggagcgc
     4201 cacagcggaa ccattgaagt gcaatccgag gcaggaaagg gcacgacatt cactgtgact
     4261 ctgccctggg atgcagatgc tgacgtgggc gagcttgcga ggcccgctac ggacaagacg
     4321 tcaacgccaa gcgcaactga aaccgctacg actgccagcg ggaggtgatc aacgtggaag
     4381 cacaaggcaa aattctgatc gtcgacgacg aactcagcgt tcgcgattcg ctgggcaagt
     4441 ggtttcgcga agagggctac gagatcggca ctgccgagag cgccagcgac gcgcttaccc
     4501 gcatggctga aaacaaatgg gacctcgcgc tggtcgacat caagatgcgc ggcaccgacg
     4561 gcatcgagct gcagcgccgc ctccatgagc tggatcccga gctgattgtg atcatcatga
     4621 ccggctacgc ctcggtcgaa acggccgtga ccgcgctgaa aaatggggcc tacgactacg
     4681 tcagcaagcc gctcgacccg gacgagatcg cgcacctggt caagaaagca ctcgcccacc
     4741 gccgtgcgga aaaagaaaac gtccgcctgc gcgaaaccgt tgccgaggcc acgcgcccgg
     4801 gcgaactcgt cggacaaagt ccggccatgc gcaaagtgtt cgaggccatc gaaaccgtcg
     4861 gccccaccga cgccaatgtg ctgatcaccg gcgagagtgg caccggcaaa gaactcgtcg
     4921 ttcgcgcgat tcacgcggcc agcctgcgtc gctttcatcc tctggtcgtc atccactgcg
     4981 gagcgctcac cgaaacgctg ctagaaagcg aactcttcgg ccacgagaag ggcgccttca
     5041 cgggagcaca atatcgcaag aaaggcaaat ttgaaatcgc cgaaggagga accgtattcc
     5101 tcgacgagat cggcgacatc agcttgaaaa cgcagaccga cctgttgcgc gtgctgcagg
     5161 agcacgagat tacccgcgtg ggcggtaacc aggtcatcaa ggtcgacttc cgctgcgtcg
     5221 ccgcgaccaa caagaatctc gagatgatga tcgaagaggg cgcattccgt cccgatctct
     5281 tctaccgtct caacgtcttt cacatcgagc tgccgccgtt gcgcgatcgc cgcga
//
LOCUS       DASPUL010000331         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1703552, whole genome shotgun sequence.
ACCESSION   DASPUL010000331 DASPUL010000000
VERSION     DASPUL010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703552"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="VEJ00_08060"
     CDS             complement(<1..905)
                     /locus_tag="VEJ00_08060"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HXX01152.1"
                     /translation="MNVVKATRQFEDWLGRRTEIVEKDLRFKHRNMKGEVFPFLRATY
                     YRWAQVWPKVCADLARAPQVLAVGDLHVENFGTWRDVDGRLIWGVNDFDEAHPLAYTN
                     DLVRLAVSAHLASEAGRLPLNRKDICDSIVDGYHQSLRAHGRAFVLEEDHKWLRLIAE
                     NELRDPVRFWLKMDALPTLKEPVPLSAIDAIEHLMPVRDVRYRIAHRVAGLGSLGHAR
                     YVAIADWNGGRIAREAKALVSSACYWAKEYEGPSEILYQTVISRAVRCPDPFVQLRGR
                     WIVRRLSPHCSRIELATLKAQGEELR"
     gene            complement(1113..1367)
                     /locus_tag="VEJ00_08065"
     CDS             complement(1113..1367)
                     /locus_tag="VEJ00_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXX01153.1"
                     /translation="MAYVITDSCIKDSLCVDVCPTDCIHPKQDEPGFEAASQMYVDPE
                     GCIDCGACVPVCTSDSIHALDDVPEEKKDFIGKNAEYFKH"
     gene            1906..2493
                     /locus_tag="VEJ00_08070"
     CDS             1906..2493
                     /locus_tag="VEJ00_08070"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXX01154.1"
                     /translation="MRQANERTTNRRVRNVLVLLVAPVLAAPTFAQEQARPATQGGRL
                     VLVSIPDRKLAVIESEKVVARFPVAVGSVASPSPIGKFRIVSRVSNPTYYRPGTVIPT
                     GKDNPVGTRWVGISQKGYGIHGTNAPKSVGHAASHGCIRLRNRDMERLFAMVRVGDAV
                     EIRDERDEQVAQIFGDSTDDAAVASAETAAAAGGQ"
     gene            2557..2724
                     /locus_tag="VEJ00_08075"
     CDS             2557..2724
                     /locus_tag="VEJ00_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01155.1"
                     /translation="MQMAITFMAVVAGLVFSLAVALLVEELIFGQVFRLLFAPARVTP
                     QTVAMKSGRKG"
     gene            2758..>3103
                     /locus_tag="VEJ00_08080"
     CDS             2758..>3103
                     /locus_tag="VEJ00_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01156.1"
                     /translation="MLALKYLLVAGGLGMILAAVSILGYDLYRELLYRRAQATPGAAL
                     PPVPQWRWRTSLALALLAWGPILLALSIVVVPSGMAGVRVSQTSGTQPGTLYPGMHFV
                     TPLVEDVALFDTR"
BASE COUNT          637 a          870 c          997 g          599 t
ORIGIN      
        1 cggagttcct caccctgcgc tttcagagtg gccagttcaa tgcgggagca gtgtggactg
       61 aggcgccgga cgatccagcg cccgcgcagt tgcacgaagg gatccggaca gcgaaccgcc
      121 cggctgatga cggtctgata gagaatctcg gagggccctt catattcctt cgcccagtag
      181 caggcggacg agaccagagc tttggcctcg cgggcgatgc ggccgccgtt ccagtcggca
      241 atagcgacat agcgggcatg acccaggctg cccagaccgg ctacgcgatg cgcgatccgg
      301 tagcggacgt cgcgcacagg cataaggtgc tcgatcgcgt cgattgcgct gagcgggacc
      361 ggctctttca aggttggaag cgcatccatt ttgagccaaa agcggaccgg atcgcggagt
      421 tcgttctcgg cgatcaggcg cagccatttg tgatcttcct cgaggacaaa cgctcgcccg
      481 tgcgcgcgca gggactggtg gtagccatcg acaatcgagt cgcagatgtc cttccggttg
      541 agcggcaggc gtcccgcctc ggaagccagg tgagcgctga cggccaggcg aaccagatcg
      601 ttggtgtagg ccagggggtg agcttcgtcg aaatcattga cgccccagat caggcggcca
      661 tcgacgtcgc gccaggtgcc gaagttctct acatgcagat cgccaacggc cagcacctgc
      721 ggcgcccggg cgagatcggc gcagaccttg ggccagacct gggcccagcg atagtaggtg
      781 gcgcgcagga aaggaaacac ctctcccttc atgttgcggt gcttgaagcg gagatctttc
      841 tcgacgattt cggtgcgccg gcccagccaa tcttcaaact gacgagtggc tttaacgacg
      901 ttcatagcgc cgaacgatag tgcgccgctg gacggaagtc aacgggatga ctgaggtcag
      961 cacgggtcag gagaaccccc gcccttatgc gtgatcggag agccggggcc ggattgcaga
     1021 tgccgcaata ggcaagaata ccgggcgttg gcgagggctg gttcaccttg catccaacgc
     1081 ccggcggtta ccgcgcaaga acgagcagaa gatcagtgct tgaagtattc cgcattcttg
     1141 ccgataaaat ccttcttttc ctccggcaca tcgtccagag catgaatcga atccgatgtg
     1201 cacaccggca cacaggcgcc gcagtcgatg cagccttcgg gatccacgta catctgggac
     1261 gctgcttcga agccaggttc atcctgcttg ggatgaatgc agtcggttgg gcagacgtca
     1321 acgcagagag agtccttgat gcaggaatcg gtaattacat aggccatgat gaaacgctct
     1381 ccagtaagag atgagcacgt cctgatcgct caaacaggag tctagagatc gcgggaaaaa
     1441 ccggcggtga ttcgcatcac aggcgattgt gcgcgagggt gggcgaagag tggaacatgg
     1501 gatgaggtaa cgtcaacgtc aggcatgatg caaatcacgc gcatctgtga cggctgtcac
     1561 agattcgaaa tggctgtagg gcttaaatag gcacaattgc actaggcctt ctagtcatag
     1621 gatcgcccgg ctccacccac agaaccccac aagccgttac cccagacggc gggagccggg
     1681 cgcttgaagg ccccatcagt agtggaatct ccctccctcc ttccgcaacg cgctcatcct
     1741 cgatttctca cgcgcactcg gcgtgtctgt gctgacacac tgtggtcgcc gccccactga
     1801 acggaccgcg gcaacacgcc aacaccatct ttgttttcat tcacttacga tttggtttcc
     1861 gtcctgccct cagtgttggc gcacgccaga ttcagggagc gggacatgcg gcaagcgaat
     1921 gaacggacga ccaacaggcg agtccggaac gttctggttt tgctcgtggc gccagttttg
     1981 gcagcgccta cgtttgcgca agagcaggct cgcccggcca cccagggcgg gcgccttgtc
     2041 ctggtgagta ttcccgaccg caaacttgct gtgattgaaa gcgaaaaagt tgttgcaaga
     2101 ttcccggtgg cggtgggttc tgtggcgagt cctagcccca tcggcaagtt tcggatcgtc
     2161 agccgggttt ccaatcccac ttactaccgt ccggggacgg tgatcccgac cggcaaggac
     2221 aatcccgtcg gcacgcgctg ggtgggaatc agccagaagg gttacgggat ccacggcacc
     2281 aacgctccga aatcggtggg acacgctgcg tcgcatggct gcatccggct gcgcaatcgc
     2341 gacatggagc gtctgtttgc catggtccga gtgggcgatg cggtggaaat tcgcgacgag
     2401 cgcgacgagc aagttgcgca gatcttcggt gacagcacag acgatgcggc cgtcgcatcc
     2461 gcggaaacgg ctgcagctgc gggagggcaa tagagttccg cgggaaagag ttccagcaga
     2521 gtgatcggaa tcaggaagac gtgacaggag ggcatcatgc agatggcaat cacatttatg
     2581 gcagtagttg caggactggt cttctcgctg gcggtggcat tgctggtgga agagctgatt
     2641 ttcgggcaag tgttccggct gctgtttgcg ccggccagag tgacgccgca aacggttgcg
     2701 atgaagtcgg gccggaaggg ctgagtttca aaacaacttc aaacaaggag aatgacgatg
     2761 ctggcgttga agtatttgtt ggttgcgggt ggcctcggga tgattctcgc ggctgtgagc
     2821 attttgggtt atgacctgta tcgggaactg ttgtaccggc gggcacaagc gactcccggc
     2881 gcggctctgc cgccggttcc gcagtggagg tggcgcactt ccctggcgct ggcacttcta
     2941 gcctggggac cgatcctcct ggcgttgagt attgtggtgg tcccgagcgg gatggcggga
     3001 gtacgagtga gccaaaccag cggcacgcag ccagggacgc tctatcccgg gatgcatttc
     3061 gtgacaccgc tggtcgagga cgtagcgctc tttgacaccc ggg
//
LOCUS       DASPUL010000332         7230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_890806, whole genome shotgun sequence.
ACCESSION   DASPUL010000332 DASPUL010000000
VERSION     DASPUL010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7230)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7230
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890806"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VEJ00_08085"
     CDS             complement(<1..882)
                     /locus_tag="VEJ00_08085"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HXX01157.1"
                     /translation="MEALQTVAVSPGWASALRTGPDTRVKITETYAALVARDAYFWTW
                     PMVNIYNRRLVMAGVREAVRAGPLVFAPLNRNAMFTDYVDPAERAVACPNQDVVYGLA
                     SIALDLSPVVVQVPDFGARFWVYQIVDLRSDSFVQLGKMYGTTPGFYLLVGPNWRGDT
                     PKGITKIFRSATNTGFSAPRVFQDDTSEDKKAVQSVLQQVLFYPVDEFDGTMKSKDWS
                     SLPKAPVQAAGDEESKWVFPEKFVDQLPLVLADAPPLPGEEARYTQVLAVVAAAQADD
                     NLKAVMTKAAVEAEEQLV"
     gene            complement(903..3257)
                     /locus_tag="VEJ00_08090"
     CDS             complement(903..3257)
                     /locus_tag="VEJ00_08090"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXX01158.1"
                     /translation="MALTEYKPGQAFPGVIGRTFDVSKPAWPAPNRAKDGAPNVLFIV
                     LDDTGFGHLGCYGSPINTPNLNALAADGLRYNNMHTTALCSPSRSCMLTGRNHHSNGM
                     SCITEGSTGYPGGNGNIPFENGMLSEILQQNGYNTYAIGKWHLTPAEQVSAAGPYDRW
                     PLGRGFERYYGFLGGDTHQYYPDLVRDNSQTEPEKTPEEGYHLTPDLVSKAKAMIADA
                     KQVAPTKPFFMYFCTGAMHAPHHVPKEWADKYKGKFDAGWDVYREQVIAKQRELGILP
                     QNAQLSRHDPDVQDWAKLPADERRLYARMMEVFAGYLEHTDHYIGELIAFLKDIGEYE
                     NTLIMVISDNGASAEGGPTGSVNENKFFNNVPDDLKQNLSALEDLGGPKYFNHYAWGW
                     THAGNTPFRRWKRETYRGGTSDPFMIIWPKGIKAKGEIRAQYCHAIDMVPTVLDALGI
                     EAPAQIRGVTQSPLEGFSLKSSFDNPKAASLHITQYFEMFGHRSLYHDGWRAVCPWPG
                     SSFTESGLTFGAPIDYNKLIELDEKGWELYNISEDFTETTNLAGKERGRLIAMIGMWY
                     VEAGKYKVLPIDSRGTQRLAEERPRIAEGRKKYVYYPGTQVVPDNSGPRLIGIAHSIS
                     VHANVPKGGAEGALLSNGGVDGGFSLYVQGGKLTYGYNYVAERHFKVQSDKPVPEGDH
                     IFSVQFAPTGPPDIPKGKGVPAKITLLIDGAPSGSGDLPVTIPLSIGLAGGICVGRDA
                     GSPVMPDYKSPFAFTGTVKKVLVDITGEAVEDKAAQMKVYLARQ"
     gene            3578..4957
                     /locus_tag="VEJ00_08095"
     CDS             3578..4957
                     /locus_tag="VEJ00_08095"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HXX01159.1"
                     /translation="MSPDTATTQSLSPAELERRTIERRAVEVAIRGIPIVAIDAIRQG
                     FLRDMQARLNDIVYYRLLPDWKFQTTTPNASTHYFYSAHSTKDGPMVLDVPAAVGAGI
                     YGQVCDIWDVPLAIVGPGGDDKGKGGKYLLLPPDYKDPLPAGCLPVRQQTYGGFWLMR
                     TIPKSAAQADVDSAISLIKKFRMHALSQSNDPPEQRFIDASGKLWDGVPHMDESFYAV
                     LAKVVNEEPVLERDLAVMNMLRSVGIEKGKGFNPDAATSAILRDAVKEAQAWFVNRLR
                     LTAASTPYWEGGRWSLPDATGMKTQFSYQSAEMLDYDGRGMLNYAAWGPPMKQDKDAP
                     SIYLQAYSDSTGQPLTGDSVYRLRVPANAPAKQYWSVTVYDLETEAFIREAAVVSLDS
                     YNQKTRKNADGSVDIYFAPHAPPGQEDNWVTTAKGRPWNPLFRLYGPEKAFFDKTWRL
                     PDFERLATK"
     gene            complement(5485..5919)
                     /locus_tag="VEJ00_08100"
     CDS             complement(5485..5919)
                     /locus_tag="VEJ00_08100"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HXX01160.1"
                     /translation="MTKTTPEQNKALVLAAFDTLFNKRDYSAASRFWSENYIQHSAHI
                     EPGRDGLFNLIRNAPADLHYEHQLIVAEGDYVIVHGRFTGTGRAVAWIAADVVRIEDG
                     RLAEHWDVLQDEATQGESKSGRPMFGNRFPELGLTQPIDQKS"
     gene            complement(5965..6597)
                     /locus_tag="VEJ00_08105"
     CDS             complement(5965..6597)
                     /locus_tag="VEJ00_08105"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HXX01161.1"
                     /translation="MKLYHWTSSPNSRRVRIFIAEKGISIPLQPVNLAEKEQFSDWFR
                     AINPRQQVPALILDDGTTIAEVPAIWRYLEETYPEPPMLGRDAKGDALVTMWERRVEL
                     EGFAPTMEGVRNAAAGLKGRALSGPHDYDQIPELVERSKRRIGDFYTDFDIRLAENAF
                     VAGTEFSAADITALVTIDFATAGLRMPIPDRASALRRWYDKIAARPSTKA"
     gene            complement(6673..>7230)
                     /locus_tag="VEJ00_08110"
     CDS             complement(6673..>7230)
                     /locus_tag="VEJ00_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX01162.1"
                     /translation="DLSAADGAHKLAKQARALVVDRLDILIANAGTAKSATLEETTVA
                     DFDRLFAVNVRAPFFLVQQLLPIMGKDGSIVLISSLAAHAVVGTLSAYAATKGAIDTL
                     VKHFAAMLGERGIRVNAVAPGVVATDMSSFTKTDAGRDFTLGIQALKRLAQPDDIARV
                     VAFLASDDARWISGDTIRADGGSKL"
BASE COUNT         1380 a         2145 c         2173 g         1532 t
ORIGIN      
        1 gaccagctgc tcctccgcct cgaccgcggc cttggtcatt accgctttga ggttgtcatc
       61 cgcttgtgcg gccgcaacca cggcgagcac ctgcgtgtac cgggcctctt caccgggaag
      121 cggcggcgca tccgccaaga cgagcgggag ctgatccaca aacttctcag gaaagaccca
      181 tttcgactcc tcgtcgccag ccgcctggac cggcgccttc ggcaggctgc tccagtcctt
      241 ggatttcatg gttccatcga attcgtcgac gggatagaag agcacctgtt gcaagacgct
      301 ttgaaccgcc tttttgtcct ccgacgtatc gtcttggaat accctcggcg cagagaaacc
      361 cgtattcgtt gccgagcgga aaatcttggt gatgcccttt ggcgtgtctc ctcgccagtt
      421 ggggccgacc agcaggtaga accccggcgt cgtgccgtac atctttccaa gttgcacgaa
      481 actgtccgag cgcaaatcga cgatctgata cacccagaag cgagcgccga agtcgggcac
      541 ctgaacgacc accggtgaca ggtcgagcgc gattgatgca agcccataca cgacgtcctg
      601 gttcgggcag gcgaccgcgc gctccgccgg atcgacgtag tcggtaaaca tggcattgcg
      661 attcagcggg gcgaacacca gcggaccagc gcgcaccgcc tctcttaccc ctgccatcac
      721 gaggcggcgg ttgtagatgt tcaccatcgg ccacgtccaa aagtaggcat cgcgcgcgac
      781 cagcgctgcg taagtctcgg taatcttcac gcgcgtgtct ggccctgtcc gaagggcgct
      841 tgcccaaccc ggagatacgg ctacagtttg cagtgcttcc atttacccta ctccttggac
      901 tttcactggc gagcgagata caccttcatt tgcgctgcct tgtcttcaac ggcctcaccg
      961 gtaatgtcca ccagcacctt cttcaccgtg ccggtgaatg cgaatggtga cttgtagtca
     1021 ggcatcaccg gcgaacctgc gtcgcgcccg acgcaaatac cgccggcaag ccctattgat
     1081 agcggaatgg tcaccggcag atcgccgctc ccgcttggcg caccgtcgat cagcaaggtg
     1141 attttcgctg gcacgccctt tcccttcgga atgtcaggcg gtccggtggg cgcaaactga
     1201 acactgaaaa tatggtcacc ctctggaacg ggtttgtcgg actgcacttt gaagtgccgt
     1261 tcggcaacat agttgtagcc gtaggtcagc ttgccccctt gcacatagag gctaaaaccc
     1321 ccgtcaacgc cgccattcga aagcagagcg ccctccgctc cgcccttggg tacgttggca
     1381 tgaaccgaaa tcgaatgcgc aataccgatt agtcgggggc ctgagttatc gggcacaacc
     1441 tgtgtgcctg ggtagtagac gtatttcttt cggccttccg cgattctcgg ccgctcttcg
     1501 gctaggcgtt gagttccccg actgtcgatc ggcaggacct tgtacttacc ggcctcgacg
     1561 taccacatgc cgatcatggc aattagccgg ccgcgctcct taccggccaa gttggtggtc
     1621 tctgtgaagt cctcgcttat gttgtaaagc tcccagcctt tctcatcgag ctcgatgagc
     1681 ttgttgtagt caatcggtgc accgaaggtt agccccgact cggtaaagga gctaccgggc
     1741 caagggcata ccgcccgcca gccgtcgtga tacagcgagc gatgtccgaa catttcgaag
     1801 tactgagtga tgtggagcga tgccgccttt ggattgtcga atgatgactt caggctgaag
     1861 ccctccagcg gagattgcgt gacgccacga atttgcgcag gtgcttcgat gcccaacgca
     1921 tccagcaccg tcggcaccat gtcgattgcg tggcagtact gcgcgcggat ctcgcccttc
     1981 gccttgatgc ccttcggcca gatgatcatg aaggggtctg aggttccacc gcggtaagtc
     2041 tcgcgcttcc agcgacggaa tggggtgttg ccggcgtgcg tccagcccca cgcgtagtga
     2101 ttgaaatact tgggtccccc aagatcttcg agggccgaga ggttctgttt gaggtcgtcc
     2161 ggtacattgt tgaagaattt gttctcgttc accgagccgg tgggcccccc ttcggcgctc
     2221 gcgccgttgt cggatatcac catgatgagc gtgttttcgt actcgccgat gtccttgagg
     2281 aaagcaatca gctcgccgat gtagtggtcg gtgtgttcga ggtagccggc gaacacctcc
     2341 atcatccggg catagagccg ccgctcgtcc gcgggcagct ttgcccaatc ctgaacatca
     2401 gggtcatgcc gcgacagttg cgcgttctga gggaggatgc ccagttctct ctgctttgcg
     2461 atcacctgct cgcggtagac gtcccagccg gcgtcgaatt tgcccttgta tttgtccgcc
     2521 cactccttgg gaacgtgatg cggagcgtgc atagcgccgg tgcagaagta catgaagaac
     2581 ggcttggtag gtgcaacctg ctttgcatcg gcaatcattg ccttcgcctt gctgaccaga
     2641 tcgggcgtga ggtggtagcc ctcttcaggt gtcttttccg gttcggtctg gctgttatcg
     2701 cgcaccaggt ccgggtagta ctgatgcgtg tcgccgccga gaaatccgta gtatcgctcg
     2761 aagccgcggc ctaacggcca gcggtcgtat ggccccgcgg cggagacctg ttcggcggga
     2821 gtgagatgcc atttaccaat cgcgtaagtg ttgtagccgt tctgctgcag gatctccgag
     2881 agcatgccgt tctcgaatgg aatgttgccg ttgcctcccg gatagcccgt cgatccttcg
     2941 gtaatgcagg acattccgtt ggagtgatga ttgcgccccg tgagcatgca cgagcgcgac
     3001 ggggagcaaa gcgccgtagt gtgcatgttg ttgtagcgga gcccatcagc ggcgagcgcg
     3061 ttcagattcg gagtgttgat tggactcccg tagcacccaa gatggccgaa gccggtgtcg
     3121 tccaggacta tgaacaagac attcggtgcc ccgtctttag cgcgattggg tgccggccac
     3181 gcgggcttcg acacgtcaaa cgtgcggcca atgacgccgg gaaatgcctg tccgggtttg
     3241 tattcagtca gtgccataag tgcctctaat ttgggcttgc cgtgagcgac agcttgacga
     3301 atgcggcgca aaggaatgcg gcgcaaagga atgtggcccg aatgctcctt cggctcgggt
     3361 agcgcggacc ctagcgatac tttggcggac gggtccatgg gaccttggtg ccatggcagc
     3421 ctcgagcctg agcgcccttc aagcagccga aaggaccatc ggaccaacgt ccaatttcga
     3481 ttccccctgc gactcttacg cttggattat ggggcccccg tgggcgcaca cacgcgtggc
     3541 agaagtctgt ccttctcact aaaggacaag gagacctatg agcccggaca ccgccacaac
     3601 gcaaagcctt tcacccgcag aacttgagcg tcgcacgatc gagcggcgag ctgtggaagt
     3661 tgcgatccgg ggaattccga tcgttgccat cgatgccatt cgtcaggggt tcctgcgcga
     3721 tatgcaggcc cggttgaacg acattgtcta ctaccgactt ctcccggatt ggaagttcca
     3781 gaccacgact cccaatgcct cgacccacta tttctatagt gcgcactcca ccaaggacgg
     3841 tccgatggtg ctggatgtgc ccgccgccgt gggcgccggg atctacggac aggtctgcga
     3901 catctgggac gtgcccctcg ccatcgttgg accgggcggt gacgacaaag gaaagggcgg
     3961 caaatacctc ctgttgccgc ctgactataa ggatccgtta cctgcgggct gccttccggt
     4021 gcgccagcag acttacgggg gcttttggct gatgcgcacc atccctaaga gcgctgccca
     4081 ggccgatgtc gacagtgcta tttcactgat caagaagttc cgcatgcatg cgctgtcgca
     4141 gtccaacgat ccgcccgagc agcgcttcat cgacgcgtcc gggaagcttt gggacggcgt
     4201 cccgcatatg gacgagagtt tctatgcggt actcgcgaag gtagtgaacg aggagccggt
     4261 actcgagcgc gacttggctg tcatgaatat gctccgctcc gtcggcatcg agaaggggaa
     4321 agggttcaac ccggacgcgg caacaagcgc gatccttagg gatgcagtga aagaagcgca
     4381 ggcttggttt gtcaatcggc tgcgcctgac ggcggcgtcg accccgtact gggaaggtgg
     4441 tcggtggtcg cttcccgacg ctaccgggat gaagacgcag ttctcctacc agagcgcgga
     4501 gatgctcgat tatgacgggc gtggaatgct caactatgct gcatgggggc cgcccatgaa
     4561 gcaggataaa gacgctccca gcatttacct gcaggcgtac agcgacagca cagggcagcc
     4621 gttgaccggc gacagcgtct accgactgcg tgtgcccgcg aatgcgccag cgaagcagta
     4681 ttggtctgtc acggtttatg acctggagac ggaagccttc atccgagagg ccgcggtcgt
     4741 cagcctcgac tcttacaatc aaaagaccag gaagaatgcc gacggctcgg tcgacatcta
     4801 cttcgccccg catgcaccgc ccggccagga agacaactgg gtaactacgg cgaagggccg
     4861 tccgtggaat cctctcttca ggctgtacgg accggaaaag gccttcttcg acaagacatg
     4921 gcggttgcca gatttcgagc gcctcgcgac gaaataatcc gtgcttgatg cccctcccca
     4981 atggcggctt ggattcgtcg aagccgggcg atcaacttga ccggctggtg cgaggaggct
     5041 ctctctcggc cgaggacgcg atttgagcgt ccaatactat ctggtcgttt ctcgaccttt
     5101 cagcggctta agtgatcgta tctcgggaaa atattggaca atcctgaggt aggtttcgca
     5161 gtgtagtcag gtagttagtg cgttttggca cagggactga aaatccgcgt gtcggtggtt
     5221 cgattccggc ccctggccac cattctcatc agtattaacg gctgaccgcc tattcattct
     5281 tggcggcgat gtttccattc ggtttccatt tctctggggc gttgggcaag acacgactag
     5341 cggaagacct ggctcagcga ctgcagatcc cttggtctcg ctggatgagc ttctgctcga
     5401 caacccaagc gcggctctca ttctccgcag tagtgacaag agcgggttcg agaaccacaa
     5461 gcgactggtg cgcgatctgg aaaatcagct cttttgatca atgggctgcg tcagcccaag
     5521 ttccgggaac cggtttccga acatcgggcg cccgcttttc gactcaccct gagtcgcctc
     5581 gtcctgaagt acgtcccaat gctcggccaa acgaccgtcc tcgatccgga cgacgtcggc
     5641 ggcgatccac gcgacggcgc gacccgtgcc ggtgaagcga ccgtgaacaa tgacgtaatc
     5701 gccttctgcc acgatgagct gatgttcgta gtgaagatcg gcaggcgcat tgcggatcaa
     5761 gttgaacaga ccgtctcgac cgggctcgat atgagcacta tgttggatat agttctctga
     5821 ccagaaccgg cttgcggcgg agtaatcgcg tttgttgaac agggtgtcaa aagccgccag
     5881 cacgagtgcc ttgttctgtt ctggtgtggt cttggtcatg tgctcctcat ccaggccggc
     5941 cggcaaaaga tccgcaggca accattaggc cttggtactg ggacgagcgg cgatcttgtc
     6001 gtaccagcgc cgcaaagcgc tggcgcggtc tgggatgggc atcctcaggc ctgcggtcgc
     6061 aaaatcgatc gtgacgaggg ccgtgatgtc agctgccgag aactcggttc ccgcgacaaa
     6121 tgcgttctca gcgagacgga tatcgaagtc ggtgtagaag tcgcctatcc gtcgcttgct
     6181 tcgctccacc aactcgggga tttgatcgta gtcatgcggg ccggacaggg cgcgaccctt
     6241 gagtcccgcg gcggcgttgc ggacgccttc catggtcggg gcgaagccct caagttcgac
     6301 ccgacgctcc cacatcgtga ccagcgcatc gcctttcgca tcacgcccca gcatcggcgg
     6361 ctcgggataa gtctcttcga gataccgcca gatcgccggg acttcggcga tcgtggtgcc
     6421 gtcgtcgagt atgagggccg gcacctgctg acgcgggttg atggccctga accagtccga
     6481 gaactgctcc ttctcagcga ggttcaccgg ctgtaatggg atggaaatgc ctttttccgc
     6541 gatgaagatc cggacgcggc gcgaattggg cgaggacgtc cagtggtata gcttcatgat
     6601 gtgttccttg ttgggtaacg ggcggccgcg cgctgccctg tgtcaggttg cgcttggcgc
     6661 ctgccgggca cctcagagct ttgagccgcc gtcggcccgg atcgtgtcgc cgctgatcca
     6721 gcgtgcatcg tcggaggcga ggaacgcgac gactctggca atatcgtccg gctgagccag
     6781 tcgctttagt gcctgaatgc caagggtgaa gtcacgtcct gcatccgtct tcgtaaagct
     6841 tgacatgtcc gtcgcaacta caccgggcgc gaccgcattg acgcgaatac cgcgctcgcc
     6901 gagcatggcg gcgaagtgct tgaccagcgt atcgatggca ccctttgttg cggcgtaggc
     6961 tgagagcgtg ccgaccaccg cgtgggccgc aagcgacgag atcagcacaa tgctcccgtc
     7021 cttgcccatg atcggcagca attgctgcac gaggaagaag ggtgcccgaa cgttgaccgc
     7081 gaaaagcctg tcgaagtccg cgaccgtcgt ctcctcgagc gtcgccgact tcgcggtgcc
     7141 ggcattagca atgaggatgt cgagtctgtc aacaactagt gctcgcgcct gcttggcgag
     7201 cttgtgcgcg ccgtcagcgg ccgacagatc
//
LOCUS       DASPUL010000333         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_891220, whole genome shotgun sequence.
ACCESSION   DASPUL010000333 DASPUL010000000
VERSION     DASPUL010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891220"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VEJ00_08115"
     CDS             <1..618
                     /locus_tag="VEJ00_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01163.1"
                     /translation="FADSDLAGPLPALIARIDWTTVWSAITSNGTGELRSSLVARPED
                     ALRHGADAVLTYMVVGTGNADFESKEVARTADVARECERIGIPLIVESLARGKNVQNP
                     GDPKWLNLHTRMAAELGADAVKTDYTGDPVTMRSVVEGCPIPILVLGGSRQGSDDEAL
                     QMIHDAVLAGAAGVFFGRNVFQAADMESFLQRARAVLDGANLVAK"
     gene            678..>2177
                     /locus_tag="VEJ00_08120"
     CDS             678..>2177
                     /locus_tag="VEJ00_08120"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXX01164.1"
                     /translation="MSLLAVDIGSSACKAVAFATTGEILARHSSGYTPEFPQPSFAEI
                     DPQIFWDAVCSCCRAVARDLASPVQALCFSSHGETFVAVDSHGRPLRRAILNQDGRAV
                     REAALCEELMGRERLFHITGLQTHPMYSVPKILWLREHEPETFASTHSFLSLIGFLLE
                     KMGLPPYVDYSLASRFLAFDIQKRCWSGEILSATGLHESCLPAPVPAGTIAGKLRSDA
                     AGQLGVPAGCPVVLGGHDQPCSALGTGVIGGGRVSDSLGTYECLLAASEAPSLNEKAL
                     QASLNTYCHVVPAKFVTLAYFPSGIMVKWFHDLLYANGAGTGGTDPAGGELESAHYEF
                     LESHVPAGPTGLGITPHLIGTCNPEFNPRARGLISGLNPGTGRAHIYKGILEGIACEL
                     VVVAEALAGAVGGFDDIYVTGGGTRSALGLQLRAAFTGRRLHVMSQQEAVCLGTAILG
                     GVAIGEYASVEQAIAALVSESRVVNPDAGLTESYRHQLRQYRQLRSAAVQ"
BASE COUNT          390 a          644 c          699 g          444 t
ORIGIN      
        1 ttcgccgatt ctgatctcgc cgggccgctt cccgccttga tcgcccggat cgactggacc
       61 acggtctggt ctgcgatcac cagcaacggc acgggagagc tgcgaagctc gctggtagcg
      121 agacccgaag atgcgttacg ccacggagcc gacgctgtct tgacctatat ggtcgtgggc
      181 accggcaacg ccgattttga aagcaaagaa gtcgcacgga cggccgacgt ggcgcgggaa
      241 tgcgagcgga tcgggatccc gctgatcgtc gagtcgctgg ctcgcggcaa gaatgtgcag
      301 aatcccggcg atccgaaatg gttgaacctg cacacccgca tggccgccga actgggcgcg
      361 gacgctgtca agactgacta cacgggcgac ccggtgacga tgcggtccgt ggtagagggc
      421 tgtccgatcc cgatcctggt gctgggcggg agccgtcaag gctccgatga cgaggctctg
      481 cagatgattc atgacgccgt actggcaggg gccgcgggag tattcttcgg ccgaaatgtg
      541 ttccaggctg ccgacatgga gtcgttcctg cagcgggcgc gcgccgtgct ggatggcgcc
      601 aatctcgtcg caaagtaaga atttcaaaac gacttcccaa ttgaccctcc ggcacgagcc
      661 gtaacggtgt cagaaccatg agcctgctgg ccgttgatat cggatcgagc gcctgtaaag
      721 ctgtagcgtt tgccaccact ggagagatcc tggcgcgaca ctcttccgga tacacaccgg
      781 aattccccca accctcgttt gctgaaatcg atccccaaat attttgggat gcggtttgtt
      841 cctgctgccg ggcggtcgcc cgagatctcg ccagtccggt gcaggctctt tgtttcagct
      901 cgcatggcga gaccttcgtt gcagtggatt ctcatggcag acctcttcgc cgggccattc
      961 tgaatcagga tggtcgagct gttcgcgaag cggcgttgtg cgaagaactc atggggcgcg
     1021 agcggctctt tcacatcacc gggctgcaga cgcatcccat gtactccgtg cccaagattc
     1081 tctggctgcg cgagcacgag cctgagacct ttgcctcgac gcactcgttt cttagcctga
     1141 tcgggtttct tctcgagaaa atggggctgc cgccgtatgt cgactattct cttgcctcgc
     1201 gctttctggc gttcgatatc cagaagcggt gctggtcggg cgagattctg agcgctacgg
     1261 ggctgcacga gtcgtgtctg ccagcgcccg ttccagcggg aaccattgcg gggaagctcc
     1321 gctcggacgc ggccggccag ctgggggttc cagccggatg ccccgtggtt ttgggcgggc
     1381 acgatcagcc ttgcagcgca ttaggcacgg gagtgatcgg cggcggccgg gtctccgatt
     1441 ctcttggtac ctatgagtgt ctgctggccg catcggaggc gcccagcctg aacgagaagg
     1501 ccctgcaggc gtcgctcaac acgtactgcc acgtggttcc cgccaaattt gtcaccctcg
     1561 cctactttcc ctcgggaatc atggtgaagt ggtttcacga tttgctgtat gccaacggtg
     1621 cgggcacggg cggtaccgat ccggctggcg gcgaactgga gtcggcccac tacgaatttc
     1681 tcgagtcgca tgtgcctgct ggaccgaccg ggctgggcat tactccacac ctgattggaa
     1741 cctgcaatcc tgaattcaac ccgcgggcgc gcgggctgat cagcggcctc aatccgggga
     1801 ccgggcgggc gcacatctat aaaggcatcc tcgaaggaat cgcctgcgaa cttgtagtcg
     1861 tcgccgaagc cctggccgga gcagtgggcg gttttgacga catttacgtt acgggcggcg
     1921 ggacgcggtc ggcgctcgga ttgcaattga gagcggcgtt tacaggccgg cgtctgcacg
     1981 tgatgagtca acaggaagcc gtctgcctgg gcacagcgat tctgggcggc gtggcaattg
     2041 gagaatatgc cagcgtggag caggccattg ccgcgttggt tagcgagagc agagtcgtga
     2101 atcccgatgc cggcctgacg gagagctatc gtcaccagct ccgtcaatac cggcagttac
     2161 gttccgccgc tgtgcaa
//
LOCUS       DASPUL010000334         4658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1286251, whole genome shotgun sequence.
ACCESSION   DASPUL010000334 DASPUL010000000
VERSION     DASPUL010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286251"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1647
                     /locus_tag="VEJ00_08125"
     CDS             43..1647
                     /locus_tag="VEJ00_08125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HXX01165.1"
                     /translation="MNISLIMFLVFIAITLGITYWASKRSAGASAYFAASRQIKGWQN
                     GIAVAGDYMSAASFLGIAGIIAFQGYDGFLYSVGWLVAYLTVLLVIAEPLRNAGKYTM
                     ADVLAYRLKPRPVRAMASLSTLTVSTFYMIAQMVGAGALVKLLLGGYVTYNRSVIGVG
                     ILMIVYVVFGGMLATTWVQIVKAILLMAGTILLSILVMAHFHFSFGEFFGAIGQVTYH
                     DAKGAVVVKDFLTPGLRYKPPYGPLDLISLGLALVFGTAGLPHILVRFYTVPDAKTAR
                     HSVVWAMALIGSFYIMTTFLGFGAATIVGPDFIKGHGGNNMSAPLLAQALAGNVFFAF
                     ISSIAFATILAVVAGLTISATTSFSHDFWTNVIHNGVERKPGEEVLVARISAFVVGAV
                     AIGIAIVLGPTFNVAFLVALAFAVAASANLPVIVLSIFWRRFNTAGAVTGLAVGLLAS
                     IGLILISPSIMGVDPPTVTGTARHLIQAKAIFPLENPGILSIPLGFLGALVGTLVSHE
                     PTSQEKFNELLVRSNTGLGAERAVAH"
     gene            1793..1868
                     /locus_tag="VEJ00_08130"
     tRNA            1793..1868
                     /locus_tag="VEJ00_08130"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1826..1828,aa:Ile,seq:cat)
     gene            2030..2488
                     /locus_tag="VEJ00_08135"
     CDS             2030..2488
                     /locus_tag="VEJ00_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01166.1"
                     /translation="MQSKLLELGIFVCVTAAVGLGQDPTKVEPKHYKLAFENDRVQVV
                     SVHYGPHEKSAMHEHPGGVVVILTEAHLRFTDENGKTREIFSKPGEARWYPPFKHRVE
                     NLGDTAYDAVYVGLKGNVVTAAEGSAVPMSPVDEKTKKLVTQLLLAAGTP"
     gene            2534..4072
                     /locus_tag="VEJ00_08140"
     CDS             2534..4072
                     /locus_tag="VEJ00_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01167.1"
                     /translation="MDRGRIARTQNMMTLLPEQSTSHWKAASLLGVVVFLSFIYFYEG
                     GGWNQNSRFDLLRAIIERHTLQIDAYHDNTRDKVHFQGHYYSDKAPGLVFLAVPFALA
                     ARPALRIAGWDPDSPRGLVALSYAVSAWAVALPTVVAGVCVFFLGLRFGGNVTAAAFG
                     AIVMCLGTPLWAYASLFWAHALVGSCFVFALVSALMLRDKGSARGDFLWALAVGLAAG
                     WATVTEYPAAPASAMLAFMALAQVWPRGAAARWRVVVGVSVGAGICLLVLLGYLHAAF
                     GSFRPSYTYYAPESFSFMQQQGYMGLTYPHPDRLLKLLFGCSRGIFFASPVLLAGPFG
                     LWWLWKERARRAAALAAAGIVAYYFLFNSSFYWWKSGLSFGPRYAGASLPLLSLGLAA
                     AWSHATPGWRRVLAGLGVCSILLALMAVSTTSQLSMQDNCPLVHSTWPAFWSGELALN
                     RESMLTVAEARSGGRYGAFNLGQVLGLSGLWSLIPLLVIWGIAAWLWTRMHRETVQPS
                     RVAD"
     gene            4227..>4658
                     /locus_tag="VEJ00_08145"
     CDS             4227..>4658
                     /locus_tag="VEJ00_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01168.1"
                     /translation="MHMQLANSFRIPFTTLMSYVPVIATVVTCFFSVILARATLRYVE
                     ATDKGLALAREEFEREWSPDLHIKVERVSATEAKVIVTNLAKTSVLLQLLQLRKLSHA
                     MPFERCRLNDPLVGGMTWTQEMGKRILACTGPEFEGPVAAAM"
BASE COUNT          819 a         1319 c         1473 g         1047 t
ORIGIN      
        1 attctcgaga cggccgagaa ctccaaagga ggcaagtaag ccatgaatat ttcactgatc
       61 atgttcctgg tcttcattgc cattacgctg ggcattacct actgggcctc caagcgatca
      121 gcgggagcga gcgcatactt cgccgccagc cggcaaatca aaggctggca aaacgggatt
      181 gcggtggccg gcgactacat gagcgcggcg tcgttcctgg ggattgccgg catcatagcc
      241 ttccaagggt acgatggctt cctgtactcg gtgggctggc tggtggcgta cctcacggtg
      301 ctgcttgtga tcgcagagcc cctgcgcaat gctggcaagt acaccatggc cgacgtgctc
      361 gcctaccggc tgaagccgcg gccggtacgg gcgatggcct cgctaagcac actgacggtc
      421 agtacctttt acatgatcgc tcagatggtg ggcgcgggcg cgctggtgaa acttctgctg
      481 ggcggatatg tcacctacaa ccgatctgtg atcggcgtcg gaatattgat gatcgtctat
      541 gtggtgttcg gcggaatgct ggccacgacc tgggttcaga tcgttaaagc catcttgctc
      601 atggcaggga cgattctgct cagcattcta gtaatggcgc acttccactt cagcttcggg
      661 gagttttttg gcgcgatcgg ccaggtgacc tatcacgacg ccaagggcgc ggtcgtggtg
      721 aaagacttcc tgacgccagg actgcgttac aaaccgccgt acggccccct ggacctgatc
      781 tcgctgggac tggcgctggt cttcgggacg gccggcctcc cgcacattct ggtacgtttc
      841 tacaccgttc ccgacgcgaa aacagcgcgg cacagcgtgg tgtgggcgat ggcactgatc
      901 ggcagcttct acatcatgac gacatttctg ggattcggag ccgcgacgat cgtgggcccg
      961 gacttcatca aagggcatgg cggaaacaac atgagtgctc cgttgctggc gcaggcgctg
     1021 gcggggaacg tgttctttgc gttcatctcg tcgatcgcct ttgccacgat tctggcggtg
     1081 gttgccggat tgacgatcag cgcgacgacc tcgttctcgc acgatttctg gacgaacgtg
     1141 atccacaacg gcgtcgagcg caagcccggc gaagaagtgc tggtagcccg catctcggcg
     1201 tttgtggtgg gcgcggtggc gatcggaatt gcgatcgtgc tgggacccac ctttaacgtc
     1261 gcgtttctgg tggctctggc gtttgccgtg gctgcttcgg cgaacctgcc ggtgattgtg
     1321 ctgtcgatct tttggcgccg gttcaacacg gcgggcgcgg ttacgggcct ggcggtggga
     1381 ttgctcgctt ccatcgggtt gattctgatt agcccgagca tcatgggggt cgatcccccg
     1441 accgtgaccg gcactgcgcg gcacctgatt caagccaagg cgatcttccc gctcgagaat
     1501 ccaggaatct tgagtattcc gctcggattc ctcggggcgc tcgtcggcac cctggtgagt
     1561 cacgaaccga cctctcagga gaagttcaac gaactgctcg tgcgctcaaa cacgggactg
     1621 ggcgcggaaa gggcggtcgc acactaagaa caggggctag aggtcagggg ctaggggcta
     1681 atcagacttg cgacagcctc tagccgcttt ctcgcgcagt aacccgatgc tcgccgaata
     1741 cttcctgacc ccctagcacc tgggccctag cccctgatag attgagtctc gcgggcccat
     1801 agctcaacgg ttagagcagc agactcataa tctgttggtt ccaggttcga atcctggtgg
     1861 gcccaccacg caaacggttg tagcacgaag ccctttcctc gcgaacatgc tcaccgcaag
     1921 cccgattaac caacatgagg catcctcctt cccgcgtcgt tgacactcct tcaggcgaaa
     1981 agtacagttg cctcagattt cctcaaaatg aagtagtggg ggagggggaa tgcagtcgaa
     2041 gttattggag ctggggattt ttgtgtgcgt cactgcagcg gtgggattag ggcaagatcc
     2101 tacgaaggtt gaacccaaac actacaaact ggcctttgag aacgaccgcg tgcaggtcgt
     2161 ttcggttcac tatggaccgc atgagaaatc ggcgatgcac gaacatccgg gaggggtggt
     2221 agtcatcctg accgaagctc accttaggtt tactgacgaa aatggcaaga caagggaaat
     2281 attctccaag ccgggtgagg cacgctggta tccgccgttc aagcatcggg tggagaacct
     2341 cggtgatacg gcctatgatg cggtctatgt cggtctgaag ggcaacgtgg tcacggccgc
     2401 agagggctcc gcggtaccaa tgagtccggt ggatgagaaa acgaagaagc tcgtcacgca
     2461 attgcttctc gcggcgggga caccgtgagc ctgttcctgg actcacagag ctagcccgcc
     2521 ggccgggcct cacgtggata gagggcgcat cgcccgcacc cagaacatga tgaccctcct
     2581 cccggagcaa tcgacttcgc actggaaagc cgcatccctg ctcggcgttg ttgtttttct
     2641 ctcgttcatc tacttctacg aaggcggcgg gtggaatcag aactcgcgct tcgacttgct
     2701 gcgcgccatc atagagcggc acacactgca gattgacgcc taccacgaca acacccgcga
     2761 caaggttcac ttccaggggc actactactc cgataaggcg ccggggctgg tatttctggc
     2821 ggtgccattc gcgctggccg cgcggcctgc gctgagaatt gcgggatggg atccggactc
     2881 gccgcgcgga ctggttgcac tttcttatgc ggtgagcgcg tgggctgtcg cgttgccgac
     2941 tgtggtggcc ggggtctgcg tgttttttct gggcttgcga ttcggcggga acgtcacggc
     3001 tgctgcgttc ggcgcgatcg tgatgtgcct tggcactccc ctctgggcat atgccagctt
     3061 attctgggcg catgcgctgg tgggatcgtg tttcgtgttc gcattggtgt cggcgctgat
     3121 gctgcgagac aagggcagcg cacggggcga ctttctttgg gcgctggctg tgggactagc
     3181 cgctggctgg gccacggtga ctgaatatcc ggctgcgccc gcctcggcga tgctcgcgtt
     3241 tatggccctg gctcaggtat ggccacgcgg cgcggcggcg aggtggcgtg tggtcgttgg
     3301 cgtgagcgtg ggcgccggaa tctgtctgct ggtgttgctc ggttatctgc acgcggcctt
     3361 tgggtcgttt cgtcccagtt acacgtacta cgcgccggag tctttttcgt tcatgcagca
     3421 gcaaggttat atgggattga cgtatcctca tcccgatcga ctactgaagc ttcttttcgg
     3481 atgctcgcgc gggattttct tcgcgtcgcc ggtgttgttg gcgggtccgt tcgggttgtg
     3541 gtggttgtgg aaggagcgag cacgtcgcgc ggctgcgctg gcagcggcgg gaattgtggc
     3601 ctactacttc ctcttcaatt cgtccttcta ctggtggaag tccggattgt cgtttggccc
     3661 gcgttatgcg ggcgcgagtc ttcctctcct ttcgcttggt ctcgccgcag cctggtcgca
     3721 cgctactcca gggtggcgcc gagtgctcgc gggactgggc gtatgtagca tcctgctggc
     3781 cctgatggcc gtgtcaacta cttcgcagct atcgatgcaa gacaattgcc cgctggtcca
     3841 ttccacatgg ccggcattct ggtcagggga gctagcgctc aatcgcgaat cgatgcttac
     3901 agttgccgaa gccagatcgg gcgggcgcta tggagcgttc aatctgggcc aggtgctggg
     3961 cctgagcggt ctttggagcc tgattccatt gctcgtgatc tggggaatcg ctgcctggtt
     4021 gtggacacga atgcaccgcg aaaccgttca gccgtcgaga gttgccgact agtccgccat
     4081 cccccgccgt ttgcatggtt accaaagggc ttggcccccg ccggttaccg tccccccacc
     4141 atgcgcatct ggagcgtcac gtcacctctg gctagactca tgcgagccga aaaagcgggc
     4201 tgagttcgcc ggagacacgc gcttctatgc acatgcagtt ggccaattcg ttccgtatcc
     4261 cgttcacgac gctgatgagc tacgttccgg tgatcgctac ggtggttacg tgctttttca
     4321 gcgtgattct ggcccgcgct acgctgcgat acgttgaggc gacggacaag ggactcgccc
     4381 tggcgcgtga ggagtttgag cgggaatggt cgcccgactt gcatatcaag gtcgagcggg
     4441 tttcggcgac cgaagccaaa gtgattgtca ccaacctggc gaaaacctcg gtcttgttgc
     4501 agcttctgca attgcgaaag ctgtcgcatg ccatgccgtt cgagcgctgc cgcttgaatg
     4561 atcctctggt cggcggtatg acctggaccc aggagatggg caagcgcatt ctggcttgta
     4621 ccggcccgga gtttgaaggg ccggtcgcgg ccgccatg
//
LOCUS       DASPUL010000335         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_333609, whole genome shotgun sequence.
ACCESSION   DASPUL010000335 DASPUL010000000
VERSION     DASPUL010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333609"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VEJ00_08150"
     CDS             complement(<1..710)
                     /locus_tag="VEJ00_08150"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HXX01169.1"
                     /translation="MRKYWDKAAAGVVSADAGRSARATRVVPASLAIGSVTIRPATVL
                     APMAGVTDTVFRRFIRNLGGCGLIMTEFTSADGVLRSKDKKAKRYLHFYEDEHPISAQ
                     LFGSNPQVMAEAARMVEGLGFDLVDLNLGCPAKKVVKCNGGSGLLRDLPAIGRIFEAV
                     RAAVKIPFSVKFRAGWNDDEIVCVELARMAESCGLCAVALHTRTREQGYSGNARWEWI
                     AAVKDAVKIPVIGNGDIRT"
     gene            complement(818..1036)
                     /gene="rpmE"
                     /locus_tag="VEJ00_08155"
     CDS             complement(818..1036)
                     /gene="rpmE"
                     /locus_tag="VEJ00_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HXX01170.1"
                     /translation="MKAAIHPAYNEVRVHCACGNTFMTRSTHKGDIAVEICSSCHPFF
                     TGKQKLVDTAGRVERFRRKYAKAETAKK"
     gene            complement(1213..>2758)
                     /locus_tag="VEJ00_08160"
     CDS             complement(1213..>2758)
                     /locus_tag="VEJ00_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01171.1"
                     /translation="ESEDPQITVNQRFAVYFPEKRPFFLENEDFFRTPMNLFFTRNIG
                     DPSAGIRLTGKDGPYSVGLMATDDRSPGLAVPNFSPLFGTRSYFTIARMSRDIFRQSS
                     VGAFYSDWECPTTGEFNRLGGLDTRLKFNANWTLDGQAVVSASDLNLNCEANLNPFNS
                     GNRGNNNYYAGPADKLELHREGLHFSYDGTYNDITPGFVTVPGFINRVDIRETVQTAD
                     YRFRPKSGWIVDWGPSAYVRYDFDHTGLRLDSDSFPYFAIQGRGQTLVTLRPYEELRE
                     RLRPQDFCFLGFSLFCAAANPGDSDFHEHTSGANVRTSYLKKATIAASYYWGDGVNFV
                     ALPATTATSQPYNRPFLARQDMTMASLTLRPIKPLKIQNTYLFDRLRSINSTFLQVQS
                     QTPGADRGIFNNHIIRSKWNWQFTPQLALRVILQYNALLAGTPGIGSPYTSLPTAKQF
                     NADFLLTYLVHPGTAIYLGYNSDLQNLNRGLAVDPATGLVLNTPRGYINDSRQFFVKV
                     SYLFRF"
BASE COUNT          531 a          849 c          812 g          566 t
ORIGIN      
        1 gtgcggatgt cgccgttgcc gatcaccgga atcttcaccg catccttcac cgccgcgatc
       61 cattcccagc gggcgttccc gctgtatccc tgctcgcgcg tgcgcgtatg caatgccaca
      121 gcgcatagcc cgcaggactc cgccatgcgc gccagttcca cgcacacgat ctcgtcgtca
      181 ttccacccgg cacgaaactt cacgctgaac ggaatcttca cggcggcccg caccgcctca
      241 aaaatcctcc caatcgctgg cagatcgcgc agcagacccg aaccgccgtt gcacttcacg
      301 actttcttcg ctgggcaccc caggttcaga tcgaccaggt cgaagccgag cccttcgacc
      361 atgcgagcgg cctcggccat cacctgcgga ttgctgccga aaagctgcgc cgagatcgga
      421 tgctcgtctt cgtagaaatg gagataccgc ttcgccttct tgtccttgga gcgcagcacg
      481 ccgtcagccg aggtgaactc ggtcatgatc agcccgcatc cgcccaggtt acggatgaac
      541 cggcgaaaga cggtgtcggt gaccccggcc atgggcgcga ggacagtcgc ggggcgaatg
      601 gtgacactgc cgatcgcgag ggaggccggc acgacccgtg tggcgcgggc actcctgccc
      661 gcgtctgccg ataccacccc cgcagccgct ttgtcccagt acttgcgcac ggtcctattc
      721 taccctccag ccaaaacgaa gcccccgcct tgaaacgggg gctcactttc ctcgattgat
      781 ccgcgtgaat ccgcgcaaat ccgtggcgaa aaggaagtta cttcttcgcc gtctcggcct
      841 tggcgtactt gcggcggaag cgctccacgc ggccggcagt gtcgaccagt ttctgcttac
      901 cggtgaagaa cggatggcaa ctcgaacaga tttccaccgc gatgtcgccc ttatgggtcg
      961 accgcgtcat aaacgtattg ccgcaggcgc agtgcacgcg cacttcgtta taggcaggat
     1021 gaatggctgc tttcataggg ttacctctga tttcccagct ctttgcaggc ttcagagcga
     1081 gtcatttgat tctaccggat ggggaaagga ttcgccaacc atcgcgctcc ctacttcgac
     1141 gcaagctgct cctgcatcca cttgagaaca atagggatca gaagcagctt ccgttcgacg
     1201 gcacttgagg gctcagaacc ggaacaaata gctcaccttc acaaagaact gccggctgtc
     1261 gttgatgtag ccccgcggcg tgttcagcac cagcccggtt gcaggatcga cggccaggcc
     1321 gcggttcaaa ttctgcaggt cgctgttgta gcccaggtag atcgccgtcc ctggatgcac
     1381 cagatacgta aggaggaaat cggcgttgaa ctgtttcgcg gtgggcaggg aagtgtaggg
     1441 cgatccgatg cccggcgtgc ccgccagcag tgcgttgtat tgcaaaatca cccgcagcgc
     1501 cagctgcggc gtgaactgcc agttccactt gctgcgaatg atgtggttgt tgaagatgcc
     1561 gcggtccgct cccggcgtct gcgattgcac ctgcagaaat gtggagttga tggaccgcag
     1621 acgatcgaac aggtacgtgt tttgaatctt cagcggcttg atgggtctca gggtcaggct
     1681 ggccatggtc atgtcttgcc gcgcgaggaa aggccggttg tacggctgcg atgtcgccgt
     1741 cgtcgccggc agggccacga agttcacgcc gtccccccag tagtagctgg ccgcgatcgt
     1801 tgccttcttc aggtagctgg tccgcacgtt ggcgccgctc gtgtgctcat ggaagtcgct
     1861 atcccctgga ttcgccgccg cacagaacaa cgaaaatccg aggaagcaga aatcctgcgg
     1921 acgcaaccgt tcccggagtt cttcgtaagg cctcagcgta actaacgtct gccctcgtcc
     1981 ctgaattgcg aaatagggaa acgaatcgct gtctaaccgc aggccggtgt ggtcgaaatc
     2041 gtaacgaacg tacgccgagg gcccccagtc cacaatccat cctgactttg gccgaaagcg
     2101 gtagtccgcc gtctggacag tctcacggat gtcgacgcga ttgatgaaac cgggaaccgt
     2161 gacgaacccg ggcgtgatgt cgttgtaggt tccgtcgtag ctgaagtgca ggccctctcg
     2221 gtgcagttcg agcttgtcgg caggcccggc atagtagttg ttgttcccgc gattgccgga
     2281 gttgaacgga ttcaggtttg cttcgcaatt cagattcaga tcgctcgcac tgactaccgc
     2341 ctggccgtcg agcgtccagt tggcgttgaa tttcaggcgt gtgtcgaggc cgcccaggcg
     2401 attgaattcg cccgtcgtcg ggcattccca gtcgctgtaa aacgctccca cgctcgactg
     2461 gcgaaagatg tcgcggctca tccgcgcaat cgtgaaataa gagcgcgttc cgaagagcgg
     2521 gctgaagttg ggcaccgcca ggccggggct gcggtcgtcg gttgccatca gtcccaccga
     2581 gtacggcccg tctttacccg ttagccgtat tcccgccgac gggtcgccaa tgtttcgcgt
     2641 gaaaaacagg ttcattgggg tgcggaagaa gtcttcgttc tcgaggaaaa acggccgctt
     2701 ctccgggaag tagacggcga accgctggtt caccgtgatc tgcggatctt ccgattcc
//
LOCUS       DASPUL010000336         7396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_57938, whole genome shotgun sequence.
ACCESSION   DASPUL010000336 DASPUL010000000
VERSION     DASPUL010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57938"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VEJ00_08165"
     CDS             complement(<1..195)
                     /locus_tag="VEJ00_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01172.1"
                     /translation="MSTRSEPGVPQEVASPLGDTLGHSLIERIGNTPMLRLDALTADL
                     PGVVLLGKAEWCNPGGSVKDR"
     gene            423..893
                     /locus_tag="VEJ00_08170"
     CDS             423..893
                     /locus_tag="VEJ00_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01173.1"
                     /translation="MEARTYEEAVAWLRDNGFDLTEAPGTHDRVFLRKYNVSAAIQKT
                     PGDGVKIFAYPGYLVGSEISKLVNRGYQQFLKTSKTEVPATADHLKALHSFAEELKVA
                     LGLPSLYNESLGTVSESYHYDRIVDRDKPAVERPKRPWEVAGKEAAVAVKKGRA"
     gene            complement(983..2299)
                     /locus_tag="VEJ00_08175"
     CDS             complement(983..2299)
                     /locus_tag="VEJ00_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01174.1"
                     /translation="MSSKAGALALIISTFFIFNAVQSPHSAPTFYNDVLPILQNHCQS
                     CHRAGEVAPMQFETYEQTRPWAHAIAHSTQMKMMPPWFADPRYGHFANDNSLTDQQIA
                     TLTAWADAAAPAGDLHEAPAPRSWTQGWNIPQPDLVVKMPKPVKIPAEGEIEYTYEIV
                     PTHFTEDRWVQMSEFLPGSPAHVHHAVVYIRPPDSQWLRHAPVGEPFTASTLSDPEDR
                     RQAHETTSDLLLVYAPGSSPDQWPDGMAKFVPAGSDLVFQVHYTTNGTADEDQTAIGM
                     VFAKTAPKQRVITLQLNNHALIIPPGAPDFRVEVEGTLPNDATLLSLFPHMHLRGKRF
                     EYDIVHDDGSAEILLRVNYHFHWQLSYKLAEPRVLKAGTKLRAIAWYDNSKNNPHNPD
                     PEKTVTWGDQTSDEMMVGFFDVAIPAAMDKWEFFVRHNADAPKKKD"
     gene            2416..3441
                     /gene="hemE"
                     /locus_tag="VEJ00_08180"
     CDS             2416..3441
                     /gene="hemE"
                     /locus_tag="VEJ00_08180"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HXX01175.1"
                     /translation="MSAPTSSFVRACRSQPVERTPVWFMRQAGRYMAEYRAVRKQHSL
                     LEICKKPKLAAEVTITAAEILGVDAAIIFADLLLPLEVMGLPFHFSAGEGPVIEKPVR
                     TKEDIARLRADRAGELGYVSEAVSLVAKHFADRVPVIGFCGAPFTLASYMIEGGGSRN
                     YVHAKKMMYNVPAEWDALMGKLVTVTSAYAVEQVRAGADAIQIFDSWVGCLSVEDYRR
                     YVLPHVTGMVKELQKTGAPIIYFGTDSATLLPAMRETGADVIGLDWRIPLDEGWARLD
                     HRCAVQGNLDPVLLFADWKELKARAEDVLRRAGGRTGHIFNLGHGILPETPVENVRNL
                     ARLVQEY"
     gene            3628..5025
                     /gene="hemG"
                     /locus_tag="VEJ00_08185"
     CDS             3628..5025
                     /gene="hemG"
                     /locus_tag="VEJ00_08185"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HXX01176.1"
                     /translation="MTRVAIIGGGISGLAAAYELEERRRGGAQVEYVLYESSTNLGGV
                     LRTEHIDGCVVEAGPDSFVTEKPWAADLCRALGIGDQLIGSNDADRKTYILVRGRLIE
                     MPDGLMFMVPTKILPTGFSPLFSWKTKLRMTQELFHPPRAVDHDESVAEFVERHYGAE
                     MVDRLADPLLSGVYGGEAANLSVRAVLPRFAEMERNYGSLGRAMLAARKKMPAGSGGA
                     APPLFTSLKNGMQSLAAAVASRLTASSIVTGVIVQALQREAGAWVVSAGMQSDQFDAV
                     IIALPALAAAQVLRLATAELAGELAAIQYTSSITVGLGYDREVRQALPPGFGFLVPRS
                     EGRRLLAATFVHNKFPHRAPEDRALLRCFLGGAIAENLWGLSNDGIIAIVREELQQIL
                     GVKAEPLFARVYKWKSAMAQYGVGHLERLERIERLRRQLPGLALAGNGYRGIGVPDCV
                     RSGREAARDVAGAAG"
     gene            complement(5177..7096)
                     /locus_tag="VEJ00_08190"
     CDS             complement(5177..7096)
                     /locus_tag="VEJ00_08190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HXX01177.1"
                     /translation="MTSESDLLTPLRRYWGYSSFRPLQERIVRSLLARHDTCVVMPTG
                     GGKSLCYQLPAVVAEQTAVVISPLIALMQDQAAQLAQMGIPAAVLNSSLSSDEQSRVM
                     RRAQDGAYRLLYLSPERLARGDTLSWLQRVPTGFFAIDEAHCISEWGHEFRPEYRQLS
                     RLRGTFPDRPIAAFTASATRHVRHDILAQLALRNPDKYIASFHRPNLRYLVRECDDGV
                     QQTALLVTALRQYPEGNVIVYSPTINRVEETVDFLEDQGIAAVAYHAKMDNTDRRRNQ
                     ERWMSDEVRVLVGTIAFGLGINKATVRAVIHMALPKSLEQFYQEAGRAGRDGNSADCV
                     LLWRKKDAGLLGFFANQITDAAERDRAWRRYHVIREFAESTRCRHHQICTYFGETPRW
                     TFCESCDVCGNTPAWLVPELLPDPLKRREFGKPSASNIPSTGTGAAGPLSPQPLNPDE
                     TGLRDYLREWRRIMAKERDVPAYVVLHDTTLEEICRKQPQSIAGLLQITGIGERKAEM
                     FGQSLLDALARFQAGARASALPEKKTAPAVETLSLLAQGKTFAEIAQIRGRQISTIVN
                     AVAALVEKGELDFEADWIDRNKQSVIEAACARLGTEWLKPLKDALPPEITYDEIRLVV
                     ARLRREPMQQKVIPA"
     gene            complement(7154..>7396)
                     /locus_tag="VEJ00_08195"
     CDS             complement(7154..>7396)
                     /locus_tag="VEJ00_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01178.1"
                     /translation="WDWGQTYFYSVPDSGLVAPGHLLSSSLRGPSWLSGGSVGPEGSA
                     LCFAVIAAASIAFARVYPRAESARSSPPSDSQMGSP"
BASE COUNT         1393 a         1976 c         2562 g         1465 t
ORIGIN      
        1 acggtccttg acagatcctc cggggttgca ccactcggcc ttgcccagta gtacaacacc
       61 gggaagatcg gcggtgagcg cgtcgaggcg cagcatagga gtgttcccga tgcgctcgat
      121 caggctgtgg ccgagcgtat cgccaagtgg tgatgccact tcctgtggaa cgccgggctc
      181 cgatcgtgta gacactgttg ccattttaat gaggtggcac tccatatttg aactgaatcg
      241 ccgtgaccaa cgtaatgtga tatgcggcca gaaacaaggt atgtagtatt ttgtttagat
      301 gatcggaatg gttcggcgat gcagttcgtg taaagatacg ctcggtcctc attgaacctg
      361 catctaatag acgagtgcgc gccgataagg aaagggtgaa gtaaagagca atgagcaaaa
      421 cgatggaagc acggacctat gaagaagcag tggcatggct gcgcgacaac gggttcgatc
      481 tgacggaagc gccgggcacg cacgaccggg tgttcctgcg caaatacaac gtgtcggcgg
      541 caatccagaa gacgccgggc gacggcgtga agatttttgc ctatcccggg tacctggttg
      601 gcagcgagat ctctaagctg gtcaatcgcg gatatcagca gttcctgaag acctccaaga
      661 ctgaggtgcc ggccacggcc gaccacctga aagcactgca ctcgtttgcg gaagagctga
      721 aggtcgcgct cggcttgccc agtctttaca acgaatcgct ggggacggta agcgagtcct
      781 accactacga ccgcattgtg gaccgcgaca agccggcggt tgagcggccg aaacgtccgt
      841 gggaagtggc ggggaaagaa gcggcggtcg cggtgaagaa ggggcgggcg taaccgagct
      901 tacccctttt ctggttaagt ttctccaccc tccgccgggc ttcgccctgg cgtgacagcc
      961 gaggcggctg tcgctacgcg agctaatcct tcttcttggg cgcgtccgcg ttgtggcgca
     1021 cgaagaactc ccacttgtcc atcgctgccg gaattgccac gtcgaaaaat cctaccatca
     1081 tttcgtcgga ggtttgatcg ccccaggtga ccgtcttttc cggatcgggg ttgtgcgggt
     1141 tgtttttcga attgtcatac cacgcgatgg cgcggagttt ggttccagcc ttcaaaactc
     1201 gtggttccgc gagtttgtaa ctcagctgcc agtgaaaatg atagttcacg cgtagcagga
     1261 tttcggcgct gccgtcgtcg tgcacgatgt cgtattcgaa gcgcttgccg cgcaggtgca
     1321 tgtggggaaa caggctgagg agtgtggcgt cgttggggag ggtgccttcg acttccacgc
     1381 ggaaatccgg cgctcccggt ggaatgatca gcgcatgatt attcagctgc agcgtaatga
     1441 cgcgttgctt cggtgccgtc tttgcgaaca ccatgccgat ggcggtctgg tcttcgtcgg
     1501 ctgtgccgtt ggtggtgtag tgcacttgaa aaaccagatc tgatcccgcg ggcacaaact
     1561 tggccatgcc gtcaggccat tggtcgggcg agctgccggg ggcgtagacg agcaggagat
     1621 cactggtcgt ttcgtgggcc tggcggcggt cttcgggatc gctcagcgtg gacgcggtga
     1681 aaggctcacc caccggggcg tggcggagcc actgcgaatc gggcgggcga atatagacga
     1741 cggcatgatg cacatgcgcg gggcttccgg gaaggaattc ggacatctgc acccagcgat
     1801 cctcggtgaa atgcgtgggc acgatttcgt aggtgtattc gatctcgcct tccgcgggga
     1861 tctttacggg cttcggcatc ttcacgacga ggtccggctg ggggatgttc catccttgcg
     1921 tccagctgcg tggagcgggc gcctcgtgca ggtcgcccgc tggcgcggcc gcatcggccc
     1981 aggcggtgag ggtggcgatc tgctggtcgg tcagggaatt gtcgttggca aagtgtccgt
     2041 agcgcgggtc ggcgaaccac ggaggcatca tcttcatttg ggtggaatgt gcaatggcgt
     2101 gtgcccacgg cctggtctgc tcgtacgttt caaattgcat gggcgcgact tcgccggcgc
     2161 ggtggcagga ctggcagtgg ttctggagga tgggaaggac atcgttgtag aaggtggggg
     2221 cggagtgtgg ggactggaca gcattgaaaa tgaaaaaggt ggagatgatg agtgccaacg
     2281 ctccagcctt ggacgacatg agctcatttt actgatttca tgaaagtcaa aggccccacc
     2341 caacgaaaac cgcgttgggt ggggcacctc gcaccctcac aaactcccat ccgaaaatgc
     2401 tacagtcaac agttcatgtc ggcacccact tccagctttg ttcgcgcctg caggtcgcag
     2461 cccgtggagc gcacgccggt gtggtttatg cggcaggcgg gacggtacat ggcggagtac
     2521 cgggcggtgc gcaagcagca ctcgctgctt gagatctgca aaaagcctaa gctggcggcg
     2581 gaagtgacca tcacggcagc ggagattctt ggcgtggatg ccgccatcat tttcgccgac
     2641 ctgctgctgc cgctcgaagt catggggctg ccttttcatt tttctgcggg cgaagggccg
     2701 gtcatcgaaa agccggtgcg gacgaaagaa gacattgcgc gactgcgtgc tgatcgcgca
     2761 ggtgaacttg ggtacgtatc cgaggcggta tcgttggtgg cgaagcactt cgcggatcgc
     2821 gtgccggtca tcggattctg cggcgcgccg ttcacgctgg cgagctacat gatcgagggc
     2881 ggaggatcgc gcaactacgt gcacgcgaag aagatgatgt acaacgtgcc ggcagagtgg
     2941 gacgcgctga tgggcaagct cgtcacggtc acgagcgcct acgctgtcga gcaggtacgt
     3001 gccggagcgg atgcgatcca gattttcgac agctgggttg ggtgcctgag cgtagaggac
     3061 tatcgacgtt acgttttgcc tcacgtcacc ggaatggtga aggagctgca gaaaactggc
     3121 gcgccaatca tttatttcgg cactgacagc gcgacgctgc taccggcgat gcgagagaca
     3181 ggagccgacg tgatcgggct ggactggcgg attccgctcg atgaaggctg ggcgcggctc
     3241 gatcatcgct gcgcggtgca ggggaatctc gatccggtgc tgctgtttgc cgactggaag
     3301 gaattgaagg cgcgggcgga ggacgtgctg cggcgggcgg ggggacggac ggggcacatc
     3361 tttaatcttg ggcatggaat tctgccggag acgccggtgg agaatgtgag gaacctggcg
     3421 cggttggtgc aggagtacta gtagtcatca aaatccccac ttctcgcgca gagaacgcgc
     3481 gagaaatggg gcacccaggg tggtcttctt cgggacggtg ggtttggtcg tgaaccgagt
     3541 caaacccccc actctagccg tatagaacgc ggctagaatg gggcacccgc ttctagtctc
     3601 tgacttcatt tctatctctt tcactttatg actcgcgttg ccatcatcgg cggggggatt
     3661 tcggggctgg ctgccgcgta tgagttggaa gagcgacggc gcggcggagc gcaagtcgag
     3721 tacgttctgt acgaatcttc cacaaatctg ggcggcgtgc tccgcaccga acatattgac
     3781 ggatgcgtcg tcgaggccgg gccggattcg tttgtcacgg aaaaaccgtg ggcggccgat
     3841 ctgtgccgag cgctgggtat cggcgaccag ctgatcggtt caaatgacgc cgatcgcaag
     3901 acctacattc tggtgcgcgg gcgcctaatc gagatgcccg atgggctgat gttcatggtg
     3961 ccgaccaaaa tcctgccgac ggggttctcg ccgctgtttt cctggaaaac caagctgcgc
     4021 atgacgcagg aattgtttca tccgccacga gcggtcgatc acgatgaatc ggtcgcggag
     4081 ttcgtagagc ggcactatgg ggcggaaatg gtggatcggc tggcggatcc gctgctgtcg
     4141 ggcgtttatg ggggagaagc tgcgaatctc agcgtgcgcg cggtgctgcc gcggtttgcg
     4201 gagatggaac gcaactacgg cagcctgggg cgggcgatgc tggcagcgcg aaagaaaatg
     4261 ccggcgggat cgggcggggc agcgccgccg ctgttcacct cactcaaaaa cggaatgcag
     4321 agtctggcgg cagccgtagc gtctcggctg accgcatcgt cgattgtgac tggcgtgatc
     4381 gtccaagctt tgcaacggga agcgggcgcg tgggttgttt cggccgggat gcagtcggat
     4441 cagttcgatg cggtaatcat cgcgctgccg gcgctggccg cggcgcaggt tttgcggctc
     4501 gcgactgctg agcttgcggg agagctggcg gcgatccagt acacatcatc gattacggtg
     4561 gggttgggct acgatcgcga ggtgcggcag gcgctgccgc cgggattcgg gtttctggtg
     4621 ccacggagtg aaggtaggcg gctgctggcg gcgacgttcg tgcacaacaa gtttccgcat
     4681 cgcgcgccgg aggatcgggc gctgctacgg tgctttttgg gcggggcgat tgccgagaac
     4741 ctttgggggc ttagcaacga cgggatcatt gcgattgtgc gcgaagagct gcagcagatt
     4801 ctgggtgtga aggcggagcc gctttttgca cgcgtctaca aatggaagtc ggcgatggcg
     4861 cagtacgggg tggggcatct cgaacggctg gagcggattg agcgtctgcg gcggcaactg
     4921 ccggggctgg cgctggctgg gaacggatat cgcgggatcg gcgtgccgga ttgcgtgcgg
     4981 tcggggcggg aggcggctcg ggatgtggcg ggagcggctg gttaggttgc ggaccggaat
     5041 caatcaaagc ttgaaccgca aagatcgcta aggaaaggcc gcgaagggcg cgaagaactg
     5101 tggacgaact cgcggcacac cttcttccac agaggtactg gggagcacag ggtaaaccgg
     5161 cagtgacact tcccgctcac gcagggatga ctttctgctg catcggctcg cggcgcaggc
     5221 gggctacgac caggcggatc tcgtcgtagg tgatttcggg cggcaaagcg tctttcagag
     5281 gcttgagcca ttctgttccc aggcgcgcgc aggctgcctc aatgacggat tgcttgttcc
     5341 ggtcgatcca gtcggcctcg aaatccaact ctcctttctc gaccagcgcg gctaccgcgt
     5401 tcacaatcgt ggaaatctgc cggccacgaa tctgtgcgat ctcagcgaag gtcttaccct
     5461 gagccaagag gctcagagtc tcgacagcgg gcgcagtctt tttttcgggc agcgcggagg
     5521 ctcgggctcc ggcttggaag cgggccagag catcgagcaa tgactggccg aacatttccg
     5581 ccttgcgctc cccgatgccc gtgatttgca agagcccggc gatggattgc ggctgcttgc
     5641 ggcagatttc ttcgagcgtg gtgtcgtgga gaaccacata ggcggggacg tcgcgctctt
     5701 tggccatgat gcggcgccac tcgcggagat agtcccgtaa cccggtttcg tcggggttaa
     5761 gaggttgagg ggaaagcggt cccgctgcgc ccgtgccggt cgaggggata ttggatgcgg
     5821 atggcttgcc gaactcgcgg cgcttgagcg gatcaggcag caactcgggc accagccacg
     5881 ccggggtgtt gccgcacacg tcgcacgact cgcagaaggt ccacctggga gtttcgccga
     5941 agtaggtgca gatctgatga tgacggcagc gagttgactc cgcgaactcg cgaatgacgt
     6001 ggtagcgccg ccaggcgcgg tcgcgttcgg cagcatcggt aatctggttg gcgaaaaatc
     6061 ccaacaggcc ggcgtctttt ttccgccaga gaagaacgca gtctgcggag ttgccatcgc
     6121 ggccggcacg gcccgcctcc tgatagaact gctcgagcga tttgggcagc gccatgtgga
     6181 tcacggcgcg caccgtggcc ttgttgatgc ccagaccgaa agcgatggtg ccgaccagaa
     6241 cgcggacttc gtcggacatc cagcgctcct ggtttcggcg gcggtcggtg ttgtccattt
     6301 tcgcgtggta ggcgacagcg gcgatgccct ggtcttcgag aaagtcgact gtttcttcga
     6361 ccctgttgat ggtgggggag tagacgatca cgttgccttc cgggtattga cgcagcgcgg
     6421 tcacgagcag cgcggtttgc tgaacgccgt cgtcgcattc gcgcacgagg tagcggagat
     6481 tcgggcggtg aaagctggca atgtatttgt cgggattgcg cagggccagt tgcgcgagga
     6541 tgtcgtggcg gacgtggcga gtggcgctgg cggtgaaggc ggcgataggg cgatcgggaa
     6601 aggtgccgcg caggcggctc agctggcggt attcgggacg gaattcgtgt ccccactcgg
     6661 agatgcagtg agcctcgtcg atggcgaaaa aaccggtcgg gacgcgctgt aaccagctca
     6721 atgtgtcgcc gcgcgctagc cgttccgggg aaagatagag aagacgatac gcgccgtctt
     6781 gtgcccggcg catgacgcgg ctttgctcgt cgctgctgag cgaactgttg agcacggcgg
     6841 cgggaatccc catctgggct agttgtgccg cctggtcctg catgagcgcg atcagcgggg
     6901 aaatcaccac ggcggtctgc tccgcaacca cggccgggag ctggtagcac agcgacttgc
     6961 cgccgcccgt gggcatgacc acgcaggtat cgtgtcgagc gagcaggctg cgcacgatcc
     7021 gctcctgcag cggacgaaac gaactgtagc cccagtagcg acgcagggga gtcagcagat
     7081 cggactcgga ggtcacgagc aacagtatag gaggtgcgga aaagccgcga tgctcagtct
     7141 gtggccgtcc aattcatgga ctccccattt gggaatcgga cggcgggctc gatctcgccg
     7201 attctgcccg cgggtacacg cgggcaaatg caatcgatgc ggcggcgatt actgcgaagc
     7261 acagcgcgct gccttctggg ccgacggagc ctccgctgag ccacgacggg ccacgcaggg
     7321 atgagctgag gaggtgaccg ggtgcgacca ggccgctgtc ggggacagag tagaaatacg
     7381 tctggcccca atccca
//
LOCUS       DASPUL010000337         4663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1015617, whole genome shotgun sequence.
ACCESSION   DASPUL010000337 DASPUL010000000
VERSION     DASPUL010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015617"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1298)
                     /locus_tag="VEJ00_08200"
     CDS             complement(<1..1298)
                     /locus_tag="VEJ00_08200"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXX01179.1"
                     /translation="MADACLRLKKWCGAMLPGQTVRGGGQRWLTRHCVSLLLLWAAVA
                     ARAQQETGDLGNVSLQELGNIQVYSASKHLQDARDAPSSVTVVTADDIQKYGYRTLAE
                     ILQSVRGFYTTYDRDYTYVGVRGFGRLGDWNSRVLVLIDGHRTNNNVAGQAMIGPEFL
                     VDVDLIDRVEIIRGPSSSLYGTDAFFAVINVITRKDRQLKGLELSFAPASYGTYQGRA
                     TYGGQYKGVGMLLSGTFYDSQGQTLFFPEFNNPATNNGVTRNTDYENFQHVVATFSYR
                     GFTLQGLFSARDKGVPTGYFGTVFNDPRTRNLDYHQFFDLSYQHSVGERWQLTARTSY
                     DQARLQAPLVVPTGVPNQPTAVDTFSFRGNWWDSEAKLSRTFLEKHTITFGSEVIDNL
                     RQDQGDYSPIGNTFDPEPASSVIWAFYGQGEFAIMRNLTL"
     gene            complement(1561..2046)
                     /locus_tag="VEJ00_08205"
     CDS             complement(1561..2046)
                     /locus_tag="VEJ00_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01180.1"
                     /translation="MILLITPSVRGQECSDSLTTATGLATHWAHDWREGATRLREQTY
                     AVAVIDQFLLESEPQESEQVLEHLETTFPVYINFAVTGMERLVREVRSALHRRKREES
                     AARRAVEEQMRSEMRETLTAMLLSCELAMSVPDIPLPAAEKIRTIDHLARELRLRLQV
                     N"
     gene            complement(2175..3599)
                     /locus_tag="VEJ00_08210"
     CDS             complement(2175..3599)
                     /locus_tag="VEJ00_08210"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXX01181.1"
                     /translation="MTSAALSPITTTPQMEPANFLNLLIVDDERSIREACREVAASLG
                     YAAYVADSAEHAYRLLETQNFDAILLDLRLPGAGGLDALRRIKERRPEAVVVVVTGYG
                     TVQSAVQAMKNGAYDYVTKPFSVDELKMLLERVASHLRLKSENRLLREKVKSKQGFGG
                     IVGRAPEMEKLYRIIAKAANSVHPVLILGESGTGKEMVARSIHYSGAFRDKPFIPVDC
                     GSLVPTLIESELFGYVKGAFTGANQSKDGLMTIADGGTIFLDEVGELPVDLQAKMLRA
                     IQEKEIRPVGSTRRVPINVRILAATNRDLEQAVTQGVFRRDLYFRLNVLSLRIPPLRE
                     RRQDIPLLIGHFLERVARTSGQEKMLSDEALKAMLAYDWPGNVRELENCLERTYAFTS
                     GPLIHITDLPAEIANLPRTDSSVASDMGRPKIIPMAELEKRTILSAISELNGDKLQAA
                     RLLGIGKTTLYRKLKDYAAEGYQS"
     gene            complement(3609..>4663)
                     /locus_tag="VEJ00_08215"
     CDS             complement(3609..>4663)
                     /locus_tag="VEJ00_08215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXX01182.1"
                     /translation="ARLAEPMRFADLMASRDHAESARVLRELFDGKRDSFQIDSDLKA
                     RDGRALCWTAWRVPGKNGSPDYALALAAETAESREAELRLRRAERLEALGRVAGGVAH
                     DFNNLLTGVLLCCDLLMAGLEPGHPSRKYAEEVRSAGTQAAGLVRQLLAMARPTDFAP
                     RVLCINEIAEGMRDLLARLIGENINLTLHLDPGLGLVRMDPAQVQQIVLNLVLNARDA
                     MPSGGRISVETSNCRVEALTGSGVCTSRVAAIPCSLLVVADNGMGMDASTRAHLFEAF
                     FTTKSAGKGTGLGLTNVHDIVTANGGLIHVESAPGQGTRVNVLLPQIPEASSASTFAN
                     GTQQEKNEGGLSQRER"
BASE COUNT          905 a         1442 c         1345 g          971 t
ORIGIN      
        1 ctgagggtaa ggttgcgcat aattgcaaac tcgccctgcc cgtaaaacgc ccagatcacg
       61 gagctcgcgg gttctgggtc gaaagtgttc ccgatcgggc tgtaatcgcc ctgatcctgc
      121 cgcaggttat cgatcacttc cgaaccaaac gtaatggtgt gtttttccag aaaagtacgg
      181 ctcagcttgg cctcgctatc ccaccaattc cctcggaacg aaaatgtgtc gactgcggtg
      241 ggttggttcg gcacaccggt gggaactacg agaggagcct gcaggcgcgc ctgatcatag
      301 gatgttctcg cggtcagctg ccatcgctca cccacagaat gctggtagct gaggtcgaag
      361 aactggtggt aatccaggtt gcgcgtccgc ggatcgttga ataccgtgcc aaaatacccc
      421 gtgggaactc ctttatcgcg cgcgctgaac aatccttgca gcgtgaatcc gcgataactg
      481 aaggtcgcta cgacgtgctg aaagttctca tagtcggtgt tgcgggtgac gccgttgttc
      541 gtcgccggat tgttgaattc cgggaaaaac agcgtctgcc cctggctgtc gtagaaggtg
      601 ccagacagca gcattccaac ccctttgtat tgtccgccgt aggtggccct tccctggtat
      661 gtgccgtaac ttgcgggcgc aaatgagagc tccagcccct tgagttgccg atccttgcgg
      721 gtgatgacgt tgatcacagc gaagaaggca tcagtgccgt aaagcgagga gcttggtccg
      781 cgaatgatct cgacccggtc aatcaaatcg acgtcgacca ggaattccgg cccgatcatc
      841 gcttggccgg caacgttgtt gttcgtgcga tgtccgtcaa tcagcaccag cacccggctg
      901 ttccagtccc cgagccgtcc gaaaccgcgc actccgacat acgtgtagtc gcggtcgtag
      961 gtcgtgtaaa agccgcgaac gctctgcagg atctcagcca gcgtgcgata accgtatttc
     1021 tggatgtcgt ccgctgtcac caccgtcacc gaagacggag cgtcccgtgc atcctgcagg
     1081 tgcttggagg cgctgtagac ctggatgttt cccaattcct gcaagctgac attccccaga
     1141 tcgccagtct cctgctgcgc cctggctgcg actgctgccc agagtagaag cagggagacg
     1201 cagtgccgcg tgagccatcg ttggcctcct ccgcgcaccg tttgtcccgg aagcattgcg
     1261 ccgcaccact tcttcaagcg cagacaggcg tctgccaaga cgcacctcct ctaaagcaaa
     1321 gactcctacc cactcttcct cgacacgatg gaggcgtctc tttagaggaa ccgtggttac
     1381 gttgcggcaa gtcctcctaa cggcggctga aggctggatg tgaaatcgtg aatctgacga
     1441 gggttggtgg caggctcaga aaaatctaat gcagaacaaa gagaaatatg ggcggtccgc
     1501 gccatagtgg gccttccctt cgtcaatgat ttgttatctc ctgccaggga ccggcgccga
     1561 tcagttcacc tgcagccgca gcctcaattc ccgcgccaga tggtcgatgg tccggatttt
     1621 ctccgctgcg ggcaagggga tgtcgggtac cgacatggcc agttcgcagg aaagaagcat
     1681 ggccgtaagg gtctccctca tttcgctgcg catttgctct tcgaccgccc gccgggccgc
     1741 agactcctca cgcttcctcc ggtgcagagc cgatcggact tcgcgcacca gccgctccat
     1801 gccggtaaca gcaaagttga tgtacacggg aaaggtagtc tccagatgct cgagtacctg
     1861 ctcgctttcc tgaggttcgc tttcgagcaa gaactggtcg atcacggcga cggcataggt
     1921 ctgctcccgc aaacgggttg caccctcacg ccagtcgtgg gcccagtgcg ttgcgagtcc
     1981 ggtggcggtc gttaaagaat cggagcattc ctggccacgt actgaaggtg taatgagtag
     2041 gatcatgctc actcaaggtg caattcttcc gccacacagc atcgcgctga aaacacggct
     2101 cttagatcag tgcgccccgg cccactctcc cgttgcagaa ctaaccccgg aaagacgagt
     2161 tcaggaacgg accgtcaaga ttgatatccc tccgccgcgt aatctttcag cttgcggtag
     2221 agcgtagttt tgccgatgcc gagcaggcgc gccgcctgca gcttgtcccc attgagttcc
     2281 gatatggcac tcaggatggt ccgcttttca agttccgcca tcggaatgat cttcggacgg
     2341 cccatatcgc tggcgacgga cgaatcggtc cgcggcaagt tcgcgatctc cgccggcaga
     2401 tctgtgatgt gaatcagcgg cccgctggta aacgcatagg tgcgctccag gcagttctcc
     2461 agttcccgca cgttacccgg ccagtcgtag gccagcatgg ccttcagggc ttcgtcgctg
     2521 agcattttct cctggcccga ggtgcgcgcc acgcgttcca gaaaatgccc gatcagcaag
     2581 gggatatcct gccgccgctc gcgaagcggc gggatgcgca agctcagcac gttgaggcgg
     2641 aaatagaggt cgcggcgaaa aaccccctgg gtgacggcct gttccagatc gcgattcgtg
     2701 gccgccaaga tgcggacgtt gatcggcacg cggcgcgtgc tgcccaccgg ccggatctcc
     2761 ttttcctgaa tcgcacgcag cattttggcc tgcagatcca ccggaagctc tcccacttcg
     2821 tcgaggaaga tggttccgcc gtcggcgatg gtcataagcc cgtccttcga ctggttggcc
     2881 ccggtgaacg cgcctttgac atagccaaaa agttcgctct cgatcagcgt ggggacgagc
     2941 gatccgcaat cgaccggaat gaagggctta tcgcggaacg cgccggagta gtgaatggaa
     3001 cgcgccacca tctcttttcc ggtaccgctt tcgcccagga tcaggacggg atgcacgctg
     3061 ttggctgcct tggcgatgat gcgatagagc ttctccatct cgggcgcccg gcccacgatg
     3121 ccgccgaatc cctgcttgga tttgactttt tcccgcaaca gccggttctc cgacttcagc
     3181 ctcaagtggc tggcgacccg ctccagcagc attttcagtt cgtctacgct gaatggcttg
     3241 gtcacgtagt cgtaggcgcc gttcttcatg gcctgcaccg ccgattgcac ggtgccgtat
     3301 ccggtcacga cgaccaccac ggcctcggga cggcgctcct tgatccggcg cagcgcgtcc
     3361 aggcctcccg cgccgggcaa gcgcaggtcg agcagaatcg catcaaagtt ctgcgtttcg
     3421 agaagccggt aagcgtgttc ggcggagtcg gcgacatagg cggcataccc cagcgaggct
     3481 gcgacttcgc ggcaggcctc gcgaatcgac cgttcgtcgt cgacgatcag gaggttgaga
     3541 aagttggctg gttccatctg cggagtggtc gtgatcgggc ttaaagctgc tgacgtcatg
     3601 gcaattcctc atctctctct ttgtgaaagt cccccctcgt tcttttcttg ctgtgtcccg
     3661 tttgcaaaag tcgaagctga acttgcttcc ggaatctgcg gcagaagcac gttcacacgc
     3721 gtgccctgac ctggagcgct ctccacatgg atcagtccgc cgttggcggt aacgatgtca
     3781 tgaacgttcg ttagcccaag tccggtgccc tttccagcgg acttggtggt gaaaaaagcc
     3841 tcgaacaggt gtgcccgagt gcttgcgtcc atccccatcc cgttgtctgc cacaacgagt
     3901 aaagagcacg gaattgctgc aacacgacta gtgcaaaccc cggacccagt aagcgcttcg
     3961 acccggcagt tgctggtctc caccgagatc cgccctccgc tgggcattgc atcccgggca
     4021 ttgagcacaa ggttgaggac aatctgttgg acctgcgcgg ggtccatcct aaccagaccc
     4081 aggccgggat cgagatgcag cgtcaggtta atgttctcgc caatcagccg cgccagcaga
     4141 tcacgcatgc cttcggcaat ctcattgatg cagagcaccc ggggtgcgaa gtccgtcggc
     4201 cgggccatgg cgagtaactg gcgcaccagc ccggcagcct gggtgcccgc actacgaact
     4261 tcttccgcat acttgcgtga cgggtgtccg ggctccagcc ccgccatcag caagtcgcaa
     4321 cacagcagga ctccggtcag caggttgttg aagtcgtgag ccacgccacc ggccactcga
     4381 cccagagcct ctaatcgctc cgcccggcgg agccgcagct cggcttcgcg gctctcagcg
     4441 gtctctgcgg ccaatgccag agcatagtcg ggtgatccgt tctttcccgg tacccgccac
     4501 gcagtccagc acaatgcccg gccatcgcgc gccttaagat cagaatcgat ctgaaagctg
     4561 tcgcgcttcc cgtcgaacaa ttcgcgcagt acccgtgcac tctcggcgtg atcccgcgaa
     4621 gccatcaggt ctgcaaaacg catgggctct gcgagtcgcg cgt
//
LOCUS       DASPUL010000338         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1978126, whole genome shotgun sequence.
ACCESSION   DASPUL010000338 DASPUL010000000
VERSION     DASPUL010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1978126"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VEJ00_08220"
     CDS             <1..1051
                     /locus_tag="VEJ00_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXX01183.1"
                     /translation="LATTRYDALLISHPPNIRYLCGFSGSAGYLLVEEARSVFFTDTR
                     YDTQAHEEVKAAKVVISRKAPLKSMADWIGIRRKRARGWDIGIEAEHFTLADRKRLND
                     QLSAGTRLRDAPTVVERMRMVKDSEELERIRAAVNLGAKLYDRALRFLRPGVKEIEMA
                     AEMEYAARQAGAEEMSFPTIIASGARSALPHGRASEARIRPGGFVVCDFGVILRGYCS
                     DQTRTVWVGTPSREARRAYEGVREAQQAASDAVRPGVTAGEVDAAARNVLKKVGLGRY
                     FTHSTGHGVGLEVHEPPRVAAGQKEVLHPGMVITIEPGVYFPGKWGVRIEDMVAVTAS
                     GRDVLTPTSKEFLAV"
     gene            1158..1652
                     /gene="accB"
                     /locus_tag="VEJ00_08225"
     CDS             1158..1652
                     /gene="accB"
                     /locus_tag="VEJ00_08225"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HXX01184.1"
                     /translation="MNQKELKELIEFLIEKDIAEFELERGDVKVRIKRAGEHTVVHAH
                     GDPRFFAVPAAPPPSPELGTVPAAAPAAAAPATKPAAEEVLHMVKSPIVGTFYEAPSP
                     GAPPFVKVGDMVEAGQVLCIVEAMKLLNEIESDVAGEIVKKLASNGQPIEYGQELFAI
                     RPKE"
     gene            1781..3136
                     /gene="accC"
                     /locus_tag="VEJ00_08230"
     CDS             1781..3136
                     /gene="accC"
                     /locus_tag="VEJ00_08230"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HXX01185.1"
                     /translation="MFKKILIANRGEIALRVICACKELGIRTVAIYSEADRNSLHVRF
                     ADEAICIGPPQLAQSYLNIPAVISAAEIANVEAVHPGYGLLAENANFAEVCETSAIKF
                     IGPRPEVTRLMGEKEKARGAMKKAGVPILPGSDGIIASEDEALEWARQVGFPVIVKAS
                     AGGGGRGMRIVRNQEELPGLFKAAQAEAAGAFGNGDLYMEKYVEHPRHIEFQVLADEH
                     GNVVSLGERECSIQRRHQKLLEESPSTQVTPELRQQVGDVLCNALAAIGYTNAGTIEF
                     LMDRDRRLHFIEMNTRIQVEHPVTEMVTDVDLVKSQILIAAGAKMSDVLMGPIVHRGH
                     SIECRINAEHPEKFTPSAGKITAFHPPGGTGVRVDTAAYAEGVIPPYYDSLIAKLIVR
                     GKDRNEAVSRMARALEMFIVEGVYTTIPLHRKILADEDFRAGKIDTGFIERFLAKNGK
                     V"
BASE COUNT          669 a          863 c         1068 g          596 t
ORIGIN      
        1 tctggccacg acccggtacg atgctctcct catcagccac ccgccgaaca ttcgctacct
       61 ttgcgggttt tccgggagcg ccggatatct gctggtagaa gaggccagga gcgttttctt
      121 caccgacact cgctacgaca cgcaggctca cgaagaggtg aaggcggcga aggtagtgat
      181 ttcgcgtaaa gcccccctga aatctatggc cgattggatc gggatccggc gcaagcgggc
      241 gcgaggatgg gacattggca tcgaggccga acacttcacc ctggccgaca ggaaacgtct
      301 caacgatcaa ttgtccgcag gaacgaggct gagagatgct ccaacggtgg tggaacggat
      361 gaggatggtc aaagacagtg aggaactgga gcgcatccgg gctgcggtga acctgggggc
      421 aaaactctac gatcgtgcct taagatttct gcggccggga gtgaaagaaa ttgaaatggc
      481 ggcggagatg gaatatgcag cgcgccaagc gggggcagag gagatgtcgt ttcccaccat
      541 catcgcctcg ggagcacggt cggctctccc gcacggacgg gcttcggagg cgcggatccg
      601 tccgggcgga ttcgtggtct gcgacttcgg tgttatactc cggggttact gttcagacca
      661 gacgcggacc gtgtgggtag gtactccttc cagagaggcg cgtcgggcgt atgagggcgt
      721 gcgggaggcc cagcaggccg caagcgatgc ggttcggcct ggcgtaacgg ctggcgaggt
      781 ggatgcggct gcccgtaacg tgttgaaaaa ggttggctta ggacgctatt tcacgcactc
      841 caccggccac ggggtgggtc tggaggttca cgaacctcca cgggtcgcgg ccgggcagaa
      901 ggaagtcctg cacccaggca tggtcatcac gatcgagccg ggcgtatact tccccggcaa
      961 atggggcgtg aggattgagg atatggtggc ggtcacagcc agtggccgtg acgtgctgac
     1021 gcctacgagc aaagagtttc tcgcagtcta aagtcttctt ggcgtcaagg tgcagccggg
     1081 catggtccgt gcccggtttc tttgcgctca ccgggttggc ggtgggctgg agcggcaagc
     1141 ggtcccaccg gatacgaatg aatcaaaaag agctgaaaga actgatcgag ttcttaatcg
     1201 agaaagacat tgccgagttc gagctggaac gcggcgacgt caaggtgcgg atcaagcgcg
     1261 cgggagagca taccgtcgtg cacgcccacg gcgacccgcg tttttttgcg gttccggcag
     1321 ctccgccgcc atcgcctgag ttgggaaccg tgcccgctgc ggcaccggcg gccgctgcgc
     1381 cagcgacgaa gccggctgcc gaagaagtac tccacatggt gaagtccccg atcgtaggca
     1441 cattctacga agccccgtcg ccgggagcgc ccccgttcgt aaaagttgga gatatggtgg
     1501 aggcaggtca ggtgttgtgc atcgtggagg cgatgaagct gctcaacgag atcgagtcag
     1561 acgtcgcggg ggagatcgtc aagaagctgg cttcgaatgg ccagcccatc gaatatgggc
     1621 aggagctgtt tgccatcagg ccgaaggagt gaggagagtc gctctccatt gttgattgtt
     1681 gatttttgat tgttgaatga aagtcctatg tgcggcggtt attcaacaat caacaatcat
     1741 caatcaacaa tttttttcga ccccctgaga gctgcatttc atgttcaaga agatcctgat
     1801 cgccaatcga ggggaaatcg cgctgcgcgt catctgcgcg tgcaaggagc tgggcatccg
     1861 gacggtcgct atctacagcg aggcggaccg gaactcgctg cacgtgcggt ttgccgacga
     1921 ggctatctgt atcgggccgc cgcagctggc gcagagttac ctgaacattc cagcggtgat
     1981 cagcgcggcc gagatcgcta acgtggaggc agtgcatccg gggtacgggc tgctggcgga
     2041 gaatgccaac tttgccgaag tctgcgagac cagtgccatc aagttcatcg ggccgcgccc
     2101 ggaagtgacg cggctgatgg gcgagaagga aaaggcccgg ggcgcgatga aaaaggccgg
     2161 cgtgccgatc ctgcccgggt cggacggaat cattgcttcc gaggatgaag ccctggagtg
     2221 ggcgcgacag gtgggcttcc ccgtgattgt gaaggcatcg gctgggggcg gcggtcgcgg
     2281 catgcgcatc gtgcgaaacc aggaagaact gcccggcctc ttcaaggcgg cgcaggctga
     2341 ggccgcgggc gcattcggca atggcgatct gtacatggag aaatacgtcg agcacccacg
     2401 gcacatcgaa tttcaggtgc tggccgacga gcacggcaat gtggtgagcc tgggcgagcg
     2461 cgagtgctca atccagcgcc ggcatcaaaa gctgctcgag gagtcgcctt cgacgcaggt
     2521 cactcctgaa ttgcggcaac aggtcgggga cgtgctctgc aacgccttgg ccgcgatcgg
     2581 atacaccaac gcgggcacaa tcgagttcct gatggaccgg gaccggcggc tgcacttcat
     2641 cgaaatgaac acgcgcatcc aggtggagca tccggtgacc gagatggtca ctgacgtcga
     2701 cctggtcaag agccagatcc tgatcgcggc gggggcaaag atgagcgacg tgctgatggg
     2761 acccatcgtc catcgcgggc attcgatcga gtgccgcatc aacgctgagc atcccgaaaa
     2821 attcacgccg tcggcgggaa aaatcactgc gtttcatccg ccgggaggaa ctggcgtgcg
     2881 cgttgatacg gcggcttatg ctgaaggtgt aattcctccc tactatgact cgctgatcgc
     2941 caagctgatc gtccgcggca aagaccggaa cgaggcggtc tcgcgcatgg ctcgggccct
     3001 ggagatgttc atcgtcgagg gtgtgtacac gacgatcccg ctgcaccgca aaattctggc
     3061 cgatgaagat ttcagggcgg ggaagattga tactggattt attgagagat ttctggcgaa
     3121 gaacggcaaa gtgtagggcg gacgcaatcg tccgccccgc gaagttcccc gcctactgcc
     3181 tttcacaggt tacgtc
//
LOCUS       DASPUL010000339         2503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_743331, whole genome shotgun sequence.
ACCESSION   DASPUL010000339 DASPUL010000000
VERSION     DASPUL010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743331"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1614)
                     /locus_tag="VEJ00_08235"
     CDS             complement(91..1614)
                     /locus_tag="VEJ00_08235"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HXX01186.1"
                     /translation="MLQTADLTIKLNQNDDVVIARVELPAGTELIKEGVRVAVNVPAG
                     HKVAIRDIAQSSPVRRYNQIIGFATRPIRGGEHVHVHNLAMGDFQRDYAYCQDYKPTE
                     FVAHPATFLGIHRADGRVATRNYVGVVSSVNCSAHVCRMIADHFKGELMADYPNVDGV
                     VPIHHKTGCGMGSTGEPVDVLRRVLAGYATHPNMHSTLMVGLGCEANQINDLLAAQGL
                     KRTEQLQTMTIQETGGTRKTVQDGIARVKELLPEANKVKREPAPVSHLILGLQCGGSD
                     GYSGISANPALGAAVDQLVRHGGTAILSETPEIYGAEHLLTRRAASREVGEKLIERIK
                     WWEEYTVRLGGEMNNNPSPGNKAGGLTTILEKSLGAATKGGTTGLMAVYEYAQRVTAQ
                     GFVFMDTPGYDPVSATGQVAGGANMICFTTGRGSAYGCKPSPSLKLATNTALFDRQEE
                     DMDINCGVIVDGKASIQEVGQQFFQMIVKTASGQPSKSEIFGYGEDEFAPWVLGPTM"
     gene            complement(1714..>2503)
                     /locus_tag="VEJ00_08240"
     CDS             complement(1714..>2503)
                     /locus_tag="VEJ00_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HXX01187.1"
                     /translation="AVFLVVLLFPFYWMAVTSIKPNAELLSTEGNPFWVLHPTFEHFS
                     KLLFHTAYPSWMWNTILISVVSTFGSLAASVFAAYAIERLRFQGSRQAGMAIFLAYLI
                     PPSILFIPLATMVFKFGIFDSKLALMLTYPTFLIPFCTWLLMGYFRTIPFELEECALI
                     DGATRWQILIKIILPLAVPGLISAGIFAFTLSWNEFIYALTFISSSENKTVPVGVITE
                     LVEGDVYHWGALMAGALLGSLPVAVMYSFFVEYYVAGMTGAVKE"
BASE COUNT          461 a          762 c          803 g          477 t
ORIGIN      
        1 cggaatggga aacgacgtcg ggttccacat cttctggaag aactcgaaca gatctttcga
       61 ggaatcttcg ctcatgcgta gagcccctga tcacatcgtg gggccgagca cccagggcgc
      121 gaactcgtct tcgccgtagc caaagatctc gctcttcgat ggctggcctg acgcagtttt
      181 gacgatcatc tggaagaact gctgtccgac ttcctggatg ctggccttgc cgtcgacgat
      241 cacgccgcag ttgatgtcca tgtcctcttc ctggcggtcg aacaacgcag tgttcgtcgc
      301 cagcttgagc gagggtgaag gcttacagcc gtaggcggag ccgcgtcccg tggtgaagca
      361 gatcatgttg gccccgccgg cgacctggcc ggtagccgag accgggtcgt agcccggcgt
      421 gtccatgaac acgaagcctt gtgcggtgac gcgctgcgcg tattcgtaga cggccatgag
      481 gcccgtggtt ccccccttcg tggcggcgcc cagggatttc tccaggatgg tcgtgaggcc
      541 gcccgccttg ttgccagggg aggggttgtt gttcatttcc ccgcccagcc tgaccgtgta
      601 ctcctcccac cacttgatgc gctcgattag cttttcgccg acctcgcggc ttgcggcgcg
      661 gcgagtgagc aggtgttcgg cgccgtagat ttcaggggtc tcggagagga tcgcagtgcc
      721 gccgtggcgc accagctgat cgacggccgc gcccagcgcc gggttagccg agattccgga
      781 gtagccatcg gagccgccgc actgcagtcc caggatcagg tgactgacgg gtgccggctc
      841 gcgcttcacc ttgttcgcct ccggcagcag ctccttcacg cgcgcgatcc cgtcctgtac
      901 cgtcttgcgg gttccgccgg tttcctgaat ggtcatggtc tgcagctgct ccgtgcgctt
      961 gagtccttga gcagccagca ggtcgttaat ctgattcgct tcgcagccga ggcccaccat
     1021 cagagtggaa tgcatgttgg ggtgtgtggc gtagcccgcc aggacgcgac gcagcacgtc
     1081 caccggctcg cctgtggatc ccatgccgca gccggttttg tgatggatcg gcacgacgcc
     1141 gtccacgttc ggatagtcgg ccatcagctc gcctttgaaa tggtcggcga tcatgcggca
     1201 gacatgcgcc gagcagttca cgctgctcac gacaccgacg tagttgcgcg tcgctacccg
     1261 gccgtccgcg cgatggatgc ccaggaaagt agcgggatgg gcgacaaact cggtcggctt
     1321 gtagtcctgg cagtaagcgt agtcgcgctg aaaatcgccc atcgccaggt tgtgcacgtg
     1381 cacgtgctcg ccgccgcgta tcgggcgggt ggcgaagccg atgatctggt tgtaacgccg
     1441 gaccggcgaa ctctgtgcga tgtcgcgtat cgccaccttg tggccggcgg gaacgttcac
     1501 agccacgcgt acgccttctt tgatcagctc ggtccccgcg ggcagctcga cgcgcgcaat
     1561 aaccacgtcg tcgttctggt tgagcttgat ggtcaaatcc gcagtctgca gcatggaaat
     1621 ctccggcagt cgagtcagac cattttagag catttttctg gggtgaccgc gccggaactg
     1681 gggccgctcc ggcgattggg cagcgtccct aattcattcc tttaccgcgc cggtcattcc
     1741 cgccacgtaa tactccacga agaacgaata catcacggcg accggcagcg agccaagcaa
     1801 ggctccggcc atgagcgcgc cccagtgata cacgtcgccc tccaccagct cggtgatcac
     1861 gcccaccggt acggtcttgt tctcggacga cgagatgaac gtgagcgcgt agatgaactc
     1921 gttccacgac agggtgaagg cgaagatgcc ggccgagatg aggcccggca ccgcgagcgg
     1981 caggatgatc ttgatcagga tctgccagcg cgtggccccg tcgatgagcg cgcactcctc
     2041 cagctcgaat gggatggtcc tgaagtagcc catcagcagc caggtgcaga acgggatcag
     2101 gaacgtgggg taggtgagca tcagcgccag cttggagtcg aagatgccga acttgaacac
     2161 catcgtggcg agcggaatga acagaatgct gggcgggatc aggtaggcga ggaagatggc
     2221 catcccagcc tggcgcgagc cctggaagcg caggcgttcg atggcgtagg ccgcgaacac
     2281 gctcgcagcc agcgatccga acgtggacac caccgagatc aggatggtgt tccacatcca
     2341 gctcggatag gcggtatgga acagcagctt gctgaagtgc tcgaaggtcg ggtgcaggac
     2401 ccagaacggg ttgccttcgg tggacagcag ctcggcgttg ggcttgatgg aagtcaccgc
     2461 catccaatag aacggaaaca gcagcaccac caggaacacc gcg
//
LOCUS       DASPUL010000340         5228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_892648, whole genome shotgun sequence.
ACCESSION   DASPUL010000340 DASPUL010000000
VERSION     DASPUL010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892648"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            679..1845
                     /locus_tag="VEJ00_08245"
     CDS             679..1845
                     /locus_tag="VEJ00_08245"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXX01188.1"
                     /translation="MRTNLATVLDPNRRSNDAGEFETALRRRIVGQDQAIEKVVEIYQ
                     MFLAGLNPPGRPVGNLLFLGPTGSGKTRVVEAMAEALFGDARACIKIDCAEFQHSHEI
                     AKLIGSPPGYLGHRETHPLLTQEALNQWFTEKLKLSILLFDEIEKASDSLWQLLLGIL
                     DKATLTLGDNRRVDLSQCIIIMTSNLGAGEMTSLVDGGFGFAPKVVEVDESFDDKIQR
                     TAVEAARRKFTPEFMNRIDKTVVFKTLRDEHLAQILDIELGMVQQRVLMAAGTNQFVF
                     NCTPAVKEYLLREGTDPRYGARHLKRAIERNLVFPLANLVATGQVKLGDFVRIDMASE
                     NKMIFVREAENTMAPVLLERYGSAAPNQPAARAARTAANNRRDFGASPSVAESK"
     gene            2011..3006
                     /locus_tag="VEJ00_08250"
     CDS             2011..3006
                     /locus_tag="VEJ00_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01189.1"
                     /translation="MNLRRVCWFALSAAALAWAAAGSLSVQIKEYEVPTPKSRPHDPA
                     LAPDGSLWYTGQGANKLGRLDPKTGAFKEYPLKTPASGPHGLVADKNGNIWFTAIAGG
                     YIGKLDPKSGEITEYRPTDKASIDPHTPAFDHNGILWFTNEETNYIGRLDPATGKMTL
                     TKVPTPHAVPYGIVVLPNGTPIFCEFGSNKLGTIDPRAMTIREYTLPNTGARPRRLAL
                     APDGTIFYTDFARGYLGHFDPAAGKLLKEWPSPGGDGSEPYGIAITDDGSVWYSESGM
                     KPNTLVRFDPRSESFSSKQIPSGGGVVRNMVATPQRQLYLACSGVNKVAVVDAAP"
     gene            3506..4936
                     /locus_tag="VEJ00_08255"
     CDS             3506..4936
                     /locus_tag="VEJ00_08255"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HXX01190.1"
                     /translation="MAFLLLTLTQANLFAQQMPYDEAGPHDQYGQPYSPDRQSGYGQP
                     YSYSGPGYSEPGYAQTTPSMQPLDAVQLEQLVAPIALYPDSLVAQILAASTYPMQVAD
                     ADRWRQAQGYAPPEQIAAGADVQNWDPSVKALTAFPQVLAQMDRNLRWTTDLGNAYYN
                     QPQDVLEAVQVMRRRAQAAGSLESTPQEVVRYDEGNIELVPANPQVVYVPAYNPWTVY
                     GEPVSPYPGFSLLDAIGSFFGSSPLRYGLGTAMAAFTHTPWGWLAWGLNWLTHALLFN
                     NSDYFSHSTTVADWGFPHAGFHAYGGHRGFDHFARVRDGGGWRRGEGGVGPWHTFARA
                     PGMHAEGWGSGRFDRGFASFRGERQSFASYNRSPERRIDTRPAFRSGVDRGWSGASNR
                     ALPSYRASAGFPRRQFDGRSPGFLSRSSAKPSRSGGFHLGGGHSQKGFGAGRSNGFHF
                     GGGHAPKGFGGSKSFGGGHGGGKHHR"
BASE COUNT         1060 a         1632 c         1541 g          995 t
ORIGIN      
        1 cgacaaagtc ggacttggaa tgaggcccga gccaccacgc ggcggctccg aaaagaaggc
       61 aagaactggc tgttatgatg cgaaccgacg aaggacgcaa gcggattcct cccctaggaa
      121 gtccctgatc gcgatactag ctgcggcctt actgacccgc tagttacgtg gggccagaca
      181 cggttgacta gtcgagtacc gtgatgcaga tctgatactc cacggcgcct taggcgatgt
      241 gtgcgagatc cccgcagttg cttggatgcc tgccgccatt ccccgaccgg ggttccgcat
      301 tgcccggtaa tcggtactcg ccgttagggt taccagtaaa tgttccttcc tccattctgc
      361 aaagtatttc tctttaagcc tgtgggaggc gtaacaactc gggctagatt taccgactcc
      421 tgccgctaac tcacgacaga tgcaagagtt cggggcaccc gcaaccggcc gataccgtgg
      481 ccaaacgctt gctcatccct caacgcctgg acttccaccc ctgggggagg cagggcgggc
      541 tggctgacag agatttgagt ccttccagaa agctggcaag agaagcggaa aaaggcctac
      601 gaggacggac agaagccagc aaagcgcgga aaagacggct aaggaaagga aagaacgtcc
      661 ggtaacggga acgaccttat gagaacgaac cttgcgacag tgctcgatcc gaaccgccgc
      721 agcaacgatg ccggagaatt cgaaaccgcg ctgcgccgcc gcattgtggg tcaggaccag
      781 gcgatcgaaa aggtagtcga gatctatcag atgttcctcg cggggctgaa tccgccggga
      841 cgccctgtgg gcaatcttct gttcctgggc cccaccggat cgggcaagac acgcgtggtg
      901 gaagccatgg ccgaggcact cttcggcgac gcgcgcgcct gcatcaagat cgattgcgcc
      961 gaattccagc actcgcacga aattgccaag ctgatcggct cgcctccggg atatctggga
     1021 catcgcgaga cccatccctt gctgacgcag gaggcgctga accagtggtt caccgagaag
     1081 ctgaagctct cgatcctgct gtttgacgaa atcgagaagg cgagcgactc gctgtggcag
     1141 cttctgctcg ggatcctcga caaggcgact ctgactctgg gcgataaccg tcgcgtcgac
     1201 ctgtcgcaat gcatcatcat catgacctcg aacctgggcg cgggcgagat gaccagcctg
     1261 gtagacggcg gattcggctt cgctcccaag gttgtcgaag tcgacgagtc cttcgacgac
     1321 aagattcagc gcaccgcggt cgaagccgcc cggcgcaagt tcacgcccga gtttatgaac
     1381 cgcatcgaca agacggtggt gttcaagacg ctgcgcgacg agcacctggc gcagattctc
     1441 gacatcgaac tcggcatggt gcagcagcgc gtgctcatgg ctgccggcac gaaccagttc
     1501 gtgttcaatt gcacgcctgc cgtgaaggaa tatctgctgc gggaaggcac cgaccctcgc
     1561 tatggagcac gtcatctgaa gcgtgcgatc gagcgcaatc tcgtgtttcc tttagccaat
     1621 ctggttgcca ccggccaggt caagctgggc gatttcgtgc gcatcgacat ggcttccgag
     1681 aacaagatga tcttcgtcag ggaagccgag aatacgatgg cgccggtcct gctcgagcgc
     1741 tacggatccg ccgctcccaa ccagcccgca gcccgtgccg cgcgcacggc tgccaacaat
     1801 cgccgcgact tcggggccag cccgtccgtg gcggagagca aatagaattt tggtaggtaa
     1861 cgtcttcgct tgaccgcatc tcgcgagacg aatccaatgg cgccggctac cctccggcgc
     1921 cttttttctg tggtcccgag ctttgattcg ggtcttttcg gacgctccct ataagtggcc
     1981 gaggactaca atctcccgag gagatcaacc atgaacctgc gcagggtgtg ctggtttgcg
     2041 ttgagtgcgg cagcgttggc atgggctgcg gcgggatcgt tgagcgtaca gatcaaggaa
     2101 tacgaagttc ccacgcccaa gtcgcgcccg cacgatcccg ctctcgcccc cgacggctcg
     2161 ctctggtaca cgggacaagg agcgaacaag cttggccgcc ttgatcccaa aactggcgct
     2221 ttcaaggagt atccgctgaa gacacctgcc tccggaccgc acggactcgt ggcagataag
     2281 aacggcaaca tctggttcac tgcgattgcc gggggctaca tcgggaagct cgatccaaaa
     2341 tcgggcgaaa ttacggagta ccgccccacc gacaaggcca gcatcgaccc gcacacgccg
     2401 gccttcgatc acaacggcat tctgtggttc accaacgaag agaccaacta catcggccgc
     2461 ctcgatccgg cgacgggcaa gatgactctg accaaggtcc cgaccccgca cgccgttccc
     2521 tacggcatcg tagtcctgcc gaacggcacg ccaatcttct gcgagttcgg cagcaacaag
     2581 ctgggcacga tcgatcccag ggcgatgacc atccgcgagt acacgctgcc caacacgggc
     2641 gctcgccccc gacggctcgc tctcgctccc gacgggacta ttttctatac cgacttcgcc
     2701 cgcggctatc taggacactt cgatccggcg gccggaaagt tgttgaaaga atggccttct
     2761 cctggaggcg atggttcaga gccatacgga atcgccatca ccgatgacgg cagcgtctgg
     2821 tacagcgagt cgggcatgaa accgaatacg ctggtccgtt tcgaccccag gtcggagagt
     2881 ttcagctcga agcagattcc ttctggtggc ggcgtggtcc gcaacatggt ggccactccg
     2941 cagcgccaac tctacttggc ctgcagcggg gtcaacaaag tcgcagtcgt cgacgccgcg
     3001 ccttaactcc tgtttcagcc tttaccgccg gggtagcttg ggagtgccac ctgatcgcga
     3061 gaggtggggg ttgcttggcg tcctttggcg gcttttcttc gcgtccctcg cggtatgacc
     3121 ttttctcgtc agctgaaaag cctggaccgg aaagatacgc aaagaacgtc cgccaagaac
     3181 gccaagaaga cacttgcccc gaacgaacag accgatttac ctgcgcagcc aaattctgtc
     3241 ttttctgaga cttccgcgat gacccagaat tgaccagcag ggcattgatc gctggcggaa
     3301 tttcaagcat cttgttgtcc ttctcgacgc atcttgatcg aacagtgcag gccggatggg
     3361 gcattctctc agctcaggtt tgcacaggtt ttggcagtac agatgctcta gccggaggtg
     3421 gcgcacgacg agaaccctga cactcggaag ctatcgccca ttgcgcactt ctcgatcggt
     3481 aaccggatcg ttgcctgcgc tcgcgatggc gtttctccta ctcaccctga cgcaagcgaa
     3541 tctcttcgcg cagcagatgc cctacgatga ggcggggccg cacgatcaat acggccaacc
     3601 gtattcccct gaccggcaat cgggctatgg gcagccttat tcgtattccg gtccaggcta
     3661 ttcagagcct ggttacgcgc aaacgacacc gtcgatgcaa cctctcgacg ccgtgcagct
     3721 cgagcaattg gtagctccca tcgcgctcta cccggattcg ttggtggcgc agattctggc
     3781 cgcttccact taccccatgc aggtggcgga cgctgaccgc tggcgccagg cacagggcta
     3841 tgcgcctccg gagcagattg cagccggagc tgacgtgcag aactgggatc cgagcgtgaa
     3901 agcgctgact gctttcccgc aagtgctggc gcagatggat cgcaacttgc gctggaccac
     3961 cgacctcggc aacgcctact acaaccagcc gcaagatgtt ctggaagccg tgcaagtcat
     4021 gcgacggcgc gcgcaagcgg ccggcagtct ggagagcacg ccccaggaag tcgtgcgcta
     4081 cgacgagggc aacatcgaac tcgtgcccgc gaacccgcag gtcgtctatg tgccggctta
     4141 caatccctgg accgtctacg gagagccggt ctcgccctat cccggcttct cgctgctcga
     4201 tgccatcgga tcgttcttcg gttcatcacc gcttcgttac gggcttggca ccgccatggc
     4261 tgccttcacc cacacgcctt ggggatggct ggcatggggc ctgaactggc tcacccacgc
     4321 tctgctgttt aacaattccg actacttctc gcacagcacc accgtcgccg attggggctt
     4381 tccgcacgcc ggatttcatg cgtatggcgg gcacagaggc ttcgaccact ttgcgcgcgt
     4441 gcgtgacggc ggcggttggc gccggggtga gggtggcgtc ggcccctggc atacattcgc
     4501 gcgagcgccc ggcatgcacg cggaaggttg gggcagcgga cggttcgacc gcgggtttgc
     4561 atcgtttcgc ggcgagcgcc agtcgttcgc cagctacaat cgttcacccg aaagacggat
     4621 cgacactcgt ccggcattcc ggtccggggt tgaccgcgga tggtcgggag cttccaatcg
     4681 cgcgctgcct tcgtatcgcg cttcggccgg ctttccacgg agacaattcg atggccgatc
     4741 ccccggcttt ctcagccgat cttccgccaa gccctcgcgt tcgggaggat ttcatctcgg
     4801 cggaggacac tcgcagaagg ggtttggcgc aggacgctcc aacggattcc acttcggcgg
     4861 cgggcatgca ccgaaaggct ttggcggaag caagagtttc ggtggcggcc acggcggcgg
     4921 aaagcatcac cgctaagcgg ccaaacccga tatccagcca agctcttctg ccagtcgacg
     4981 tccgaaaagc agctgcccgg gcgtatccaa gcagccgagt gatgaccttc gtaatcgcgc
     5041 cgggtccctc ccgcattgct gctgcccaac cccgctccta gaatctcagc agggtttact
     5101 gcgctctctt tccgcatgcc tgagatcaca aagcgactgg accgggccga aatcggcaaa
     5161 cgcgtggagc gcgccgaaaa gctcctgcaa aagggcaaga ctgcagaggc gctcgacgaa
     5221 tatgtcca
//
LOCUS       DASPUL010000341         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_205855, whole genome shotgun sequence.
ACCESSION   DASPUL010000341 DASPUL010000000
VERSION     DASPUL010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205855"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /gene="gpmI"
                     /locus_tag="VEJ00_08260"
     CDS             <1..1454
                     /gene="gpmI"
                     /locus_tag="VEJ00_08260"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HXX01191.1"
                     /translation="PEGQMGNSEVGHLNIGAGRIVHMDITRIDLMVQNGELFQHPVLL
                     DAMKHARTGERRLHLFGLVSDGGVHSQQEHLYALLKMAKQNGLERVYVHAFMDGRDTL
                     PTNGAGYLEQLQQKMREYNCGKIATINGRYYAMDRDRRWERIAKAYNAMVFADAEGGR
                     QADPVQGMKDSYNKGVTDEFVVPFVCTDKSGQPLATIRDDDSCICFNFRADRVRQITR
                     ALTRNSGLNEKGGGDLPGAADLDATIPRDRVPKNLHYVCMTQYDKNFSLPVVIPPESL
                     ANILANVMANQKMRNLRVAETEKYAHVTYFFNGGVEQPFPGEDRILVPSPKVATYDLK
                     PEMSAAGIADAVVKATEEGTFDVMIVNFANADMVGHSGKIEPTVKAVETVDACLARVE
                     KAVRAKGGAMLITADHGNAELMIDPATGGPHTAHTTNPVPFIVVSENAKQFTLKSGGS
                     LRDISPTILGMLGLDEPKEMTGVDLRHFSAASR"
     gene            complement(1538..2092)
                     /locus_tag="VEJ00_08265"
     CDS             complement(1538..2092)
                     /locus_tag="VEJ00_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01192.1"
                     /translation="MTQNNDHAMMEEVVLPEHLLPLGSVLGQGVTPLDLCMVLAKIFR
                     VQYTEVALLRLEKGLLRFIFPEYLRTTGAIPLSSKAVAAHTALSKKAEIFNNFARVKH
                     ASIFETIKPEASDPSEEAPPPTPIQKLMSVPIMDREANVMGVIQISRKGLDPKFAQDF
                     SREDLHDLELAAGLLSGAPALLQN"
     gene            2383..2679
                     /locus_tag="VEJ00_08270"
     CDS             2383..2679
                     /locus_tag="VEJ00_08270"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HXX01193.1"
                     /translation="MSLPTQPNVKLNNAPDYRENYANSVQMRVNIWDFFLVFGTLQQQ
                     TETQVEIRNFQGIYLSPQQAKALLGLLQQNVAGYESAFGEIKLDPRMSPAGPVH"
     gene            2727..>2977
                     /locus_tag="VEJ00_08275"
     CDS             2727..>2977
                     /locus_tag="VEJ00_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01194.1"
                     /translation="MRKGLSAKIRENWGLIFAHPALTFAIILVTMFLLHAPLLQLPYF
                     WDEAGYYVPAARDLLLTGSLIPHSTLSNAHPPLVLAWLAL"
BASE COUNT          652 a          944 c          807 g          574 t
ORIGIN      
        1 tgcccgaagg ccagatgggc aacagcgaag tcggccatct caacatcggc gccggccgca
       61 tcgtgcatat ggacatcacc cgcatcgacc tgatggtcca gaacggcgag ctgttccagc
      121 atccagtgct gctcgatgcc atgaagcacg cgcgcacagg cgagcgccgc ctgcatctgt
      181 tcgggttggt ctccgatggt ggcgtacact cgcagcagga gcatctctat gcgctcctga
      241 agatggcaaa gcagaatggc ctggagcgtg tctacgtcca cgccttcatg gacggccgcg
      301 acacactgcc taccaatggc gctggctatc tcgaacagct gcagcagaag atgcgcgagt
      361 acaactgcgg caaaatcgcc accatcaacg gccgctacta cgccatggac cgcgaccgcc
      421 gctgggagcg catcgccaag gcctacaatg ccatggtctt cgccgacgct gaaggcggca
      481 ggcaggcaga tcccgtgcag gggatgaaag attcgtacaa caaaggcgtg accgacgagt
      541 tcgtcgtgcc cttcgtatgc accgacaaga gcggccaacc cctcgccacc attcgggacg
      601 acgattcctg catctgcttc aacttccgtg ccgaccgcgt ccgccagatc acgcgcgctc
      661 tcacgcgcaa cagcggattg aacgaaaaag gcggcggcga tcttcccggt gcggccgatc
      721 tcgacgccac gatcccccgc gaccgcgtcc cgaaaaatct ccactacgtc tgcatgacgc
      781 aatacgataa gaatttctcg ctgccggtgg tgattccccc cgaatcgctg gccaacatcc
      841 ttgcaaatgt gatggcgaat cagaagatgc gcaacctgcg cgtcgccgag accgagaagt
      901 acgcgcacgt cacgtatttc ttcaacggcg gcgtcgagca gccttttccc ggcgaagacc
      961 gcattctcgt accgtcgccg aaagtcgcga cttacgatct gaagccggaa atgagcgccg
     1021 ccggcatcgc cgacgcggta gtaaaagcca cagaagaagg caccttcgac gtgatgatcg
     1081 tgaacttcgc caacgccgac atggtcggcc actccggcaa gatcgagccc acggtgaaag
     1141 ccgtcgaaac cgtcgacgcg tgcctcgccc gcgtcgagaa ggcagtccgc gctaaaggcg
     1201 gagctatgct catcaccgca gaccacggca atgccgagct catgatcgac cctgccaccg
     1261 gcggtccgca cacggcgcac actaccaacc cggttccgtt tatcgtggtc tcagaaaatg
     1321 cgaagcagtt cactctaaag tccggtggct cgctgcgcga catttcgccc accatccttg
     1381 gaatgctcgg actcgatgaa ccaaaagaaa tgacaggggt ggacttgcgg catttctcgg
     1441 ctgcttcccg ctgaaaaaat caaaaccaca gatgacacgg cggttcacag agcagtttct
     1501 ctgtgcttag ccgtgtcctc tgtggtgaat cggtgaccta gttctggagc agcgccgggg
     1561 cgccggacag caatccggcg gcaagctcca ggtcatgcaa gtcttcgcgg ctgaagtcct
     1621 gggcgaactt cgggtctagt cctttgcggg agatttgaat cacgcccatt acgttcgctt
     1681 cgcgatccat aatcggtacc gacatcaact tttgaatggg cgtcggcgga ggcgcttcct
     1741 ccgacggatc gctggcttcc ggcttgatgg tttcgaagat gctcgcgtgc ttcacgcgcg
     1801 cgaagttgtt gaagatctct gctttcttgc tcagcgccgt gtgcgccgcc accgctttgc
     1861 tcgacagcgg aatcgcaccg gtcgttcgca gatactcagg gaaaataaag cggagcaggc
     1921 ctttctccag ccgcagcagc gcgacttcgg tgtactgcac acgaaaaatc ttggccagca
     1981 ccatgcacaa gtcgagtgga gtcacaccct ggcccagcac cgaccccagg ggcagcagat
     2041 gctcgggaag aaccacctct tccatcatcg cgtgatcgtt gttttgggtc ataagacgcc
     2101 caccgtctcc gctcttcgcg gatcgaacac tcaacatatc gcacttttcc cgccacaaga
     2161 tacttcctaa agtaacgggg gcggcaactg caagtccttt gttttcatcg gcgcattacg
     2221 ccaagtggcc ggccctgccc ggtaaccgag acctccgggc cagccacaaa ccatcaaccc
     2281 gatgacagtt tctgtcactt gcaccagaat caaggcactt ggttggggca cggtgtcggg
     2341 gtctttccct ctgggcagaa aactcattac actggcgagc atatgagcct accgacacag
     2401 ccgaatgtga aactgaacaa cgctcctgac tatcgcgaga actacgccaa cagcgtgcag
     2461 atgcgggtga atatctggga cttcttcctc gtcttcggaa ctctgcagca acagaccgaa
     2521 acccaggtcg agatccgcaa ctttcagggc atctatctca gcccgcagca ggccaaggca
     2581 ttgcttggac tcctgcagca gaacgttgcc ggatacgaga gtgcattcgg tgaaatcaag
     2641 ctcgaccccc gcatgagccc ggccggaccg gtgcactaac aaattctttg ccacggattc
     2701 cacggattca cgcggattat agaaaaatgc gaaaaggact atccgcgaag atccgcgaaa
     2761 attgggggct gatcttcgcg catccagctc tcacgtttgc gatcatcctc gtcacgatgt
     2821 tcctgctgca cgcgcccttg ctgcagcttc catacttttg ggatgaggcc ggctactacg
     2881 ttcctgcggc ccgcgacctt ctgctgaccg gaagcctgat cccgcactca actctctcca
     2941 atgctcatcc cccgctggta ctggcgtggc tcgcgct
//
LOCUS       DASPUL010000342         2879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_892906, whole genome shotgun sequence.
ACCESSION   DASPUL010000342 DASPUL010000000
VERSION     DASPUL010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892906"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..2768)
                     /locus_tag="VEJ00_08280"
     CDS             complement(213..2768)
                     /locus_tag="VEJ00_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXX01195.1"
                     /translation="MTGQLVGHYRVLEKIGAGGMGEVYRARDERLGRDVALKLIRPAS
                     SENPDLLRRFEQEARAAAALNHPNILAIYDVGFERSTPYIVTELLQGKPLRQRLSEGP
                     VPLREASDYALQIAHGLTAAHDRHIVHRDLKPENLFVTNEGRVKILDFGVAKLQASPE
                     DGRAIESLTTVTKSGTVVGTVAYMSPEQLRGKPVDHRSDIFTFGAILYEMLSGQRAFR
                     GETEVDTMTAVLRENPPEANLEKAEIPLGYRDIVRHCLEKEPENRFQTARDLAFAVQT
                     ISGSTTGAQVRVPSPTARTHSALPWAMVAALAIAIVVLALIQLRAPVPAPIYQRLTFE
                     SGTVYAARFTPDGQAIVYSAAWNGKPVQLFSTVGHSLQAQQLALSDAGLLSISRSNEL
                     AVVLHGNHSGQLETVNGMLASTPLAGGSPHELLSDVRWADWDASGKLAVVHYVDGHSR
                     LEYPVGTVLHQSDGWISHIRFSPRGDQIAFLDHPALYDDRGAVCIVDVAGHARTLSPE
                     WESASGLAWSPGGNEIWFGAANQGNKLDLQAVSLSGKTRPVLALPEAFTLQDVAADGR
                     VLVTLNSKRLAMAEGELGRNEDVDLSWHDWNIAKDISSDGQSVLFEDASEVAGTGYSV
                     ALRKLDGSLPIRIGEGSAGGLSPDGKWAISVSTGNPEQVTLLPVGAGQPRPVNVSGLP
                     HVQNGWARFLADSTAIIINGNETGRAARCYLLSLSGAKPRAVTPEGIRCGPASPDGRF
                     VVGVGPDLAISLYPVDGGSARPVPGSEPGFRPVQWSTNGSALYGYHEGELPAKIYKID
                     IASGRKTMLQELRPGVPAGIVNISPVVVSRDGKRFAYSYNQTLSVLYLISGLR"
BASE COUNT          571 a          845 c          928 g          535 t
ORIGIN      
        1 gaacccggct gccgcattac cgcgaggcgc tgggtgaggg gcagacttca acgtcgatct
       61 tcttccacta tgtgccggct gatttcgcgg ggtcgctgga ctaaaccagg gactggaagc
      121 aaaagggcga cgctcagggg ctaaagccca cgtttcatgc aaatgcggcg gcgcgcccct
      181 tcaaaacgcg aggcggttct ccgcagagcg tgctagcgca agccggagat gaggtagaga
      241 accgacagcg tctgattgta actgtaggcg aagcgtttgc cgtcccgact tactaccacg
      301 ggtgaaatgt ttacgatgcc ggcgggaact ccgggacgca actcctgaag catggtcttt
      361 cgacctgaag cgatgtcgat cttgtagatc ttggcgggca gctcgccctc atgatatccg
      421 tacaaggcag aaccattcgt tgaccattgc acgggacgaa atccgggttc actcccggga
      481 acgggtcgcg ctgagccgcc atccactggg tacaacgaaa tagccaagtc cggaccgacc
      541 ccaacgacaa accggccgtc aggagatgca ggaccgcaac gaatgccttc cggtgtcacg
      601 gcacggggct tggcgcccga cagcgacaga agataacagc gagcggcacg gccggtttca
      661 ttcccattga tgatgatcgc agtgctgtcg gccaggaagc gcgcccatcc gttttgaacg
      721 tgagggagcc cgctcacatt aacggggcga ggctggccgg cgccgactgg cagcaaggtt
      781 acctgctcgg gattgcccgt cgacaccgaa atcgcccatt tcccatcggg ggagaggccg
      841 cctgcgcttc cctcgccaat gcggatgggc aagctgccgt ccagtttgcg cagggccacg
      901 gaatagccgg tgcccgcgac ttcgctggcg tcctcgaaca gcacggactg gccatcgctg
      961 gaaatatctt tggcgatatt ccagtcatgc cacgagaggt cgacatcctc gttgcgcccc
     1021 aattctccct cggccatggc caggcgcttg gagttcagcg tgaccagcac gcgtccgtcg
     1081 gcagcgacgt cctgtaaggt aaaggcctcc ggcagagcca gcacaggccg ggtttttccc
     1141 gagaggctca ccgcctgcag atcaagcttg ttgccctgat tggctgcgcc aaaccagatc
     1201 tcgtttccgc ccggactcca ggccaagccg ctggccgact cccattccgg agagagagta
     1261 cgcgcgtggc ctgccacgtc aacgatgcag acggcgccgc gatcgtcgta cagggctgga
     1321 tgatcgagaa atgcgatctg gtcgccgcgt ggggaaaaac gaatgtggct gatccatcca
     1381 tcgctctgat ggagcacggt tccgacggga tactccagcc ggctgtggcc atcgacgtag
     1441 tggacaacgg caagctttcc ggaggcatcc cagtcggccc agcgaacgtc ggaaagaagt
     1501 tcgtgcggcg atcctccggc cagcggcgtg ctcgcgagca ttccgttgac agtttccagt
     1561 tggccgctgt gatttccgtg aagcacaacc gccagttcat tgctgcggga tatggacagc
     1621 agaccagcgt cgctcaaggc cagttgttgg gcctgcagcg aatgcccgac cgttgagaac
     1681 agttgtaccg gcttgccgtt ccaggcagcg ctatagacga tcgcctggcc atcgggggtg
     1741 aagcgagccg cataaacggt tcctgattcg aaggtcaggc gctggtaaat cggagcagga
     1801 acgggagcgc gaagttggat cagcgccagc accacgatcg cgatggccaa cgccgcgacc
     1861 atcgcccagg gcaaggcgga atgggttcga gctgtcgggc tgggaacgcg gacttgtgcg
     1921 ccggtcgtcg aacctgagat cgtctgcacc gcgaaggcga gatcgcgagc cgtctggaat
     1981 cggttctccg gttctttttc caggcaatga cggacgatgt cgcggtatcc gagcgggatc
     2041 tcggcctttt ccaagttggc ctcaggagga ttttcccgca agaccgcggt catcgtgtcg
     2101 acttctgttt cgccccgaaa agcgcgctgg ccgctcagca tttcgtagag aatggcgccg
     2161 aaggtgaaaa tgtcgctgcg gtgatcgacc ggtttgccgc gcaactgttc aggcgacatg
     2221 taggcgacgg tacccaccac agtgccgctc ttggttaccg tcgtcaggct ctcaatggcg
     2281 cggccgtctt cgggagacgc ctggagcttg gctacaccga agtccagaat cttgactctt
     2341 ccctcattgg tgacgaaaag gttctccggc ttcaggtcgc ggtgcacgat gtgccggtca
     2401 tgagcggctg tgaggccgtg cgcgatctga agggcgtagt cactggcctc gcgcagcgga
     2461 acggggcctt cggacaggcg ctgccgcagt ggcttgccct ggagcaattc ggtgacgatg
     2521 taaggagtgc tgcgctcgaa accgacgtcg tagatggcga ggatattggg atggttcaga
     2581 gcagcggcag cgcgcgcttc ttgctcgaag cggcggagca ggtcgggatt ttcgctggag
     2641 gcgggccgaa tcagcttcaa cgcgacgtcg cggccgagcc tctcgtcgcg ggcgcgatac
     2701 acttcgccca tgcctcccgc gccgatcttt tcgaggaccc ggtagtgccc gacaagctgg
     2761 ccagtcatag agaagaatgc aggggcgcga cttgcctagg gatgttagga aggaatacgc
     2821 gtggagtcaa taccagcaag gggagccgcg tccgatacaa tacggggcgt ggagcaatc
//
LOCUS       DASPUL010000343         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1287582, whole genome shotgun sequence.
ACCESSION   DASPUL010000343 DASPUL010000000
VERSION     DASPUL010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287582"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VEJ00_08285"
     CDS             <1..256
                     /locus_tag="VEJ00_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01196.1"
                     /translation="GRVRTVPMPTWVKVAVDTWTSAAGIADGHVFRAVNRGGKVQPAA
                     LKEKVVWQLLQGYAAIAGVPGIAPHDLRSYAECRIMPNAY"
     gene            complement(324..1322)
                     /locus_tag="VEJ00_08290"
     CDS             complement(324..1322)
                     /locus_tag="VEJ00_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXX01197.1"
                     /translation="MSRPLDFPELLQAFFSHRLIALRQASPHTIASYRDTFRLLLQFA
                     HKTLGKPASKITLENLNATFIGAFLDSLEHDRSNGVRTRNLRMAVIRSFFRYAALENP
                     QHSGLIQRVLAIPSKRHERRLVGFLARAEIDALLTVPNRDSWRGRRDHALLLLAVQTG
                     LRLSEITALRQQDVVLGRAAHVRCVGKGRKERCTPIVKSTAAVLAEWIREQGPGSTQI
                     LFPNAQGGRLSSDAVQCLVYKYAAAAARTCPTLKAKRVTPHVLRHSAAMELLQAGVDR
                     SVIALWLGHESVETTQIYLDADLALKEQALKKANPLDATALRFRPDDQLLTFLKSL"
     gene            complement(1319..2251)
                     /locus_tag="VEJ00_08295"
     CDS             complement(1319..2251)
                     /locus_tag="VEJ00_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXX01198.1"
                     /translation="MTSLQQGAEDYLALRRSLGFKLKRPCRFIRDFVTWLERSGESRI
                     TIRLALEWATQPQHLQRAEWAARLSAIRGFARYWSGIDTATEVPPDGLLPFRARRATP
                     YLYPNGEIEGLLQAARCMPAQFELQPATYYCLLGLLAVSGLRISEALKLERRDIDWTE
                     GLLTIRGTKFGKSRLVPLHPTTKQVLAEYAARRDRLFPAHPAAAFFPSKTGTYLDFGQ
                     VRRVFYRLSRQIGIRGTLASHGPRLHDLRHRFAVESLLRWYRAGEDVKQRLPILSTYL
                     GHSHPTDTYWYLSNTPELMAAAGSCIEKRWEVQA"
     gene            complement(2248..3471)
                     /locus_tag="VEJ00_08300"
     CDS             complement(2248..3471)
                     /locus_tag="VEJ00_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXX01199.1"
                     /translation="MQRLIDPKLLQRLQVGPLAQYFDAYLARIEHDGFLASSVPCQAY
                     AITRFSRWLKQKGLRPDDLDESTVRKFLGRDPGVAHYPERATIRRLVLILRELGVVKA
                     DAPLPLTPPQVCIEEYRRYLVRDRGLSQASLPNYLSFVHQFLHERFPQGELCLAELRA
                     VDVITFVKAHAVKLSPGRKKLLVTALRAFLRYLQHQGKIEVGLAACVPPVASWSFSAI
                     PKSLPHGTVQRVLAGHDRSTPVGQRNYAILLLLARLGLRAGEVGGLNLEDLDWDKGLV
                     QIRRKGSRWTQLPLPVDVGAAIAVYLQSGRPPCSSRRVFLRHRAPIRGFARNGCVSSI
                     VHRTLIAAGVDSERKGAHLFRHTLAVDLLRNGASLDEIGNVLGHRSPNTTALYAKVDV
                     AALRSLALPWPGGAR"
     gene            3598..3771
                     /locus_tag="VEJ00_08305"
     CDS             3598..3771
                     /locus_tag="VEJ00_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01200.1"
                     /translation="MRHYRRTCAKLCRAAGGELEQIQLLLGHASVQTTERYLGTKQDL
                     VHAPNDGIKLRVV"
BASE COUNT          840 a         1172 c         1212 g          758 t
ORIGIN      
        1 tggccgagtg cggacggtgc caatgccaac ttgggtgaaa gtggccgtcg atacctggac
       61 gtccgcagcc ggtatcgctg acgggcatgt cttccgagcc gtaaatcgtg gcggcaaggt
      121 gcagccggcc gcactcaaag agaaggttgt gtggcaactg ctccagggat atgccgccat
      181 cgctggcgtt ccggggatcg cgcctcacga cctcaggagt tatgccgagt gccggattat
      241 gccgaatgct tattgatgtt gtcggtttgg cgcagggttc gcgagctgac tagcccggac
      301 cattcggcat aatcggccga gagctacagg cttttcagaa aagtcagcag ctggtcatca
      361 gggcggaaac ggagcgccgt tgcgtccaga gggttggctt tcttgagcgc ctgctccttg
      421 agggcgagat cggcatcaag atagatttgg gttgtttcga cggattcatg tccgagccaa
      481 agcgcgatca ccgaacggtc gacacctgct tgtagtagct ccatagcggc gctgtggcga
      541 agtacgtgtg gcgtcacccg cttcgctttc agagtcggac aagtccgcgc ggctgctgcg
      601 gcatacttgt agaccaggca ctggacagcg tcggaactga ggcggccccc ctgtgcattc
      661 gggaacaaga tctgcgtgct cccaggcccc tgctcacgaa tccactctgc cagcacagcg
      721 gcggtggatt ttacaatggg cgtacaacgc tctttgcgtc cctttcccac gcagcgaacg
      781 tgcgcagccc tgccgaggac cacgtcctgc tggcgaagag ccgttatttc cgacaatctt
      841 aagccggtct gaacagccag cagcaggagt gcatgatcgc gccgtcccct ccaggaatcc
      901 ctgttgggca ccgttaaaag cgcgtcgatt tccgcgcgcg ccagaaatcc aaccagtcgg
      961 cgctcatggc gcttactggg aatggcgagc acccgctgga taagcccgga gtgctgcgga
     1021 ttttccagtg ctgcgtatcg aaagaaggac cgaattaccg ccatgcgaag gttccgagtt
     1081 cgcacgccat tgctgcgatc gtgttcgagg ctgtcgagga acgcgccgat gaacgtggcg
     1141 ttcaggtttt ccaacgtaat tttcgaagct ggcttaccga gcgttttgtg ggcgaattgc
     1201 agcaggagac gaaacgtatc gcggtatgag gcaatcgtgt ggggactcgc ctgacgcaaa
     1261 gcgatcagac ggtgtgaaaa gaacgcctgt aggagttcgg ggaagtcgag cgggcggctc
     1321 atgcctggac ctcccaccgc ttttcgatgc agcttccggc ggccgccatc agctccggag
     1381 tgttgctaag gtaccaatac gtgtcggtcg gatgactatg gccgagatag gtcgataaaa
     1441 tcggtaaccg ctgcttgacg tcttcgcccg ctcggtacca tcgcagcagg ctttcaacgg
     1501 cgaaccgatg ccggagatcg tgcaggcgtg gtccgtggct cgccaacgtt ccccggatgc
     1561 caatttgccg agacagccga taaaagaccc gcctgacttg cccaaaatcc agataagtac
     1621 cagtcttgga aggaaagaag gccgccgccg ggtgagccgg gaagagacga tctcgtcgcg
     1681 cggcgtattc ggcgagcacc tgtttcgttg tcggatgcaa cggcacaagc cgggacttgc
     1741 cgaacttcgt gccgcggatc gtgagcaatc cctctgtcca gtcaatatcg cgacgctcca
     1801 gtttcagtgc ttcactgatc cgcaggccgg aaactgccaa gaggcccagc agacagtaat
     1861 atgtggcagg ctgcagctcg aactgagccg gcatgcatct ggccgcctgc aacagtcctt
     1921 cgatctcgcc attcgggtac agatacggcg tggcacgccg ggccctgaac ggcaagaggc
     1981 cgtccggcgg cacctcggtg gctgtatcga tcccactcca gtaccgcgca aagccacgga
     2041 tcgcgctgag gcgggctgcc cactcagcac gctgcagatg ctggggctgc gtcgcccatt
     2101 ccagtgcaag ccggatcgtg atccgtgatt caccgctacg ctcaagccac gtgacaaagt
     2161 cgcggataaa gcggcaaggt cgttttagct tgaaccccag gcttcgcctc aaggccaggt
     2221 aatcttcagc accttgctga aggcttgtca tcgggcacct cctggccagg gaagcgcgag
     2281 gctgcgaagt gcagccacgt cgaccttcgc atatagcgcc gttgtattgg ggctgcgatg
     2341 gccgaggacg tttccgatct cgtccaagga agccccgttc cgcaataggt caacggctaa
     2401 ggtgtggcga aacagatgcg ctcccttccg ttcagagtca acacctgccg cgatcagggt
     2461 ccggtgaaca atggaggaaa cacaaccgtt acgagcaaag cctcggatcg gtgcgcgatg
     2521 gcgcagaaag acacgacggc tggagcatgg cggccgaccc gattgtaggt aaaccgcgat
     2581 ggcagcacca acatcgaccg gcaggggcaa ttgggtccag cggctgccct tgcgacgaat
     2641 ttggacgagg cccttatccc aatcgaggtc ctccagattc aaaccgccga cctccccggc
     2701 tcgcaacccc aagcgggcca acagcaacaa aatcgcgtag ttccgctggc caactggggt
     2761 gctccgatca tgcccggcaa gaacccgttg cacggtcccg tgcggcaaag actttgggat
     2821 tgccgaaaac gaccagctcg ccaccggagg aacgcaggca gccaatccca cttcgatctt
     2881 tccctgatgc tgcagataac gcaggaatgc gcgaagcgca gtgacaagga gtttttttcg
     2941 ccctggggat agtttcacag catgggcctt cacgaatgtg atcacatcga ctgcgcgcag
     3001 ttccgccaga cacagctcgc cctgcgggaa ccgctcatgc aaaaactggt ggacaaacga
     3061 gagatagttc ggtaggcttg cctgagacag cccgcggtcg cgaaccagat accgccggta
     3121 ttcctcgatg cacacctgcg ggggcgtcag cggcagtggc gcatcagctt tgaccacgcc
     3181 caactcgcga aggatcaaga ccaggcgacg gatggtcgca cgttctggat agtgtgcgac
     3241 gcctggatcg cggccgagaa acttgcgcac agtgctttcg tcgagatcgt cggggcggag
     3301 gcctttctgc ttcagccatc tactgaacct ggtgatggcg tatgcctggc atggcaccga
     3361 cgatgccagg aagccatcgt gctcaattcg agcgagatag gcgtcgaagt attgggccag
     3421 cggacccacc tggaggcgct gcagtagctt gggatcaatg agacgttgca tccatcaagg
     3481 atggaggctt cactgccgga ttatgccgcg tgttcagagc cgaaccaagc caacgacaat
     3541 gcccggacga cttgattcgg cataatccgg gactcggcat aacgacgata atgccgcatg
     3601 cggcattaca ggcgcacatg cgcgaagctg tgccgcgcgg ccggtggcga gttggaacag
     3661 attcagctgc tgctcggcca tgcttccgta cagaccaccg agcgctacct cggcaccaag
     3721 caggatctgg tgcatgcgcc gaacgatggg atcaaactga gggtagtgta atcctctgct
     3781 cgcaccgcaa cggctcccac atttcctgcc ataaagccga cttgcggttc ctgatccacg
     3841 taaactcggg aatgccccct ggcgggcagc gatccgcgag ttggcggccg aacgctggct
     3901 gcacactacc gcctcaccac atgtggtcag ccgttgatca ccaggcggcg gtaggcttgt
     3961 cgtaggaatg tcaatgattt ca
//
LOCUS       DASPUL010000344         5150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1287599, whole genome shotgun sequence.
ACCESSION   DASPUL010000344 DASPUL010000000
VERSION     DASPUL010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5150)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5150
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287599"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1332
                     /locus_tag="VEJ00_08310"
     CDS             22..1332
                     /locus_tag="VEJ00_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX01201.1"
                     /translation="MGGFAGMATALHREDFHETSAAVGPQVQPGMNRVVLLLTLSILI
                     NYIDRSNLSIAAPLLQDELHISSTQLGTLLAAFFWTYGLMQIPAGWLVDHLDVKWVFA
                     AGFFVWSAATGLTSTLHGFAALIAVRVILGVGESVAFPSCSKILSTYFPEARRGFPNA
                     LLMAGLSLGPALGILVGGTVVGRVGWRPFFLALGLGGLLWIAPWIAWMPRRPARVRSS
                     STPSVGILSILRQPTAWGTCLGQFCVNYFLYFLVTWLPSYLKRGRGFSLAEVGRYGGL
                     LFLMSAIAATFAGRLADQWINAGATATVVRKGTLVAGQIGIGVCLVLTPLTHGGVFIV
                     MLAMTGVFLGIGCCSTWAVTQTLAGPLAAGRWTGVQNFVGNMAGWVAPVLTGFLVDRT
                     GRFERAFFITAAIAWTGALSWGLLVGPVEPVNWKQLSSPREAIS"
     gene            1520..2077
                     /locus_tag="VEJ00_08315"
     CDS             1520..2077
                     /locus_tag="VEJ00_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HXX01202.1"
                     /translation="MAQEIEIKFRVRDLRALNRRLQALGFRLVTRRTHEMNTLYDLPG
                     EVLRKRRQLLRLRQYGSQWTLTHKRGTTRGHHSSRVELETSVRDGKQMDAILGALGYS
                     PSFRYEKFRAEWADGKGQVVVDETPIGNFCEIEGPPRWIDATAKKLGVKSTVYITKNY
                     ASLFLEWKHETGSAAEEMTFKAVKR"
     gene            complement(2426..2914)
                     /locus_tag="VEJ00_08320"
     CDS             complement(2426..2914)
                     /locus_tag="VEJ00_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01203.1"
                     /translation="MEFEYEISADDYAAASVLHCRLNRNFNRDGVWFVGGACLLAVAL
                     VERERGLSPILLGAIGVWWMWAGLGRIFPAMFWRYYRNYYRRLGLEGKRYRANLTADG
                     FQVTGENRTWNKHWTEVSPKGEDARVFMFYSQGTLFIFAKRYLADEQQRTLRTLAGLP
                     AV"
     gene            complement(2946..>5150)
                     /locus_tag="VEJ00_08325"
     CDS             complement(2946..>5150)
                     /locus_tag="VEJ00_08325"
                     /inference="COORDINATES: protein motif:HMM:NF044398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HXX01204.1"
                     /translation="IIPKLEKGEEIEGPPGEPPQYTGDYVVDEKHRNITVTDEGWEKV
                     EGLLGIGNIADPENWGWKHHVETAVKAHALYRKDVEYVIKDGEVIIVDEFTGRMMPGR
                     RWSDGLHQAVEAKENVKIERENQTLATITFQNYFRMFKKLAGMTGTAETEATEFDKIY
                     KLDVVVIPTNKVLLRIENPDVIFRTEKEKYFAVADEIQQLAAKGQPVLVGTTSVEKSE
                     RLSDLLKKKAIKHVVLNAKFHEREAEFVAQAGRKGMVTIATNMAGRGTDILLGGNFEF
                     MAKQEMVKKGIAQQLRVAQGKIEGPQEDGESSIFYYNGNEYLVPADKWTEALNRYKEQ
                     TDKEHDEVTSVGGLHILGTERHESRRIDNQLRGRAGRQGDPGSSRFYLALEDDLMRIF
                     IPKEWVSKMLQRLGMEEGVPIESKMMTRRVETAQKAVEGQHFESRKHLLEYDDVMNKQ
                     REAVYGLRRRLLEGMDQKDLILEDYVSAILGDLLEQYCPEKAHVDDWNVKGLKDAIFT
                     RFGVDILAEGIKPETLNRGELGDAIFAKLKERYEAKEKLIGPEAMRHHERMIMLSVID
                     QQWKDHLLSMDHLKEGIGLRGYGQHDPLVEYKKESFEMFEAMMQRFQEDTVRYLYLMQ
                     ILERPREEGPGGPSAPPPGGPAGQSPDAGVPVPRGGDGNGRRPPHLVSTSADELEEAF
                     MRRKRRELEQARMAGAGDAAPVQQVVRSQEKVGRNDPCPCGSGKKYKKCHGA"
BASE COUNT          909 a         1565 c         1499 g         1177 t
ORIGIN      
        1 agtactagca ggagtgtaca attgggcggt tttgccggca tggccacggc cctccatcgc
       61 gaagacttcc atgaaacctc cgccgcagtc gggccgcagg ttcagccggg aatgaaccgc
      121 gtagttctcc tgctaaccct ctccattctc atcaactaca tcgaccgtag caacctgtca
      181 atcgctgcgc cgctgcttca ggacgaactg cacatctcca gcacgcaact tggcaccctg
      241 ctcgccgcgt tcttctggac ctacggcctg atgcagatcc cggcgggctg gctcgtcgac
      301 catttggatg tgaaatgggt ttttgccgcc ggcttcttcg tttggtccgc agccaccggg
      361 ctcacaagca ccctccatgg ttttgccgcg ctgatcgcag tccgcgtgat ccttggagtg
      421 ggggaatcgg tggcctttcc ctcttgttcc aagattctct caacatattt cccggaagct
      481 cgccgtggct ttcccaatgc gttgctcatg gccgggcttt ccctcgggcc tgctctgggc
      541 attctcgtgg gaggaacggt ggtgggaaga gttggctggc gtccgttctt tctcgccctg
      601 ggactcggag gtttgctctg gatcgcgcca tggatcgcct ggatgccgcg caggcctgca
      661 cgagtccgtt cttcctctac cccctcggtt ggcatcttgt ctatcctgcg gcaacccacc
      721 gcgtggggga cctgtctggg gcagttctgc gtcaactatt tcctgtattt cctggtgaca
      781 tggttgcctt catatctcaa gcgggggcgc ggattctcgc tggctgaggt cggaagatac
      841 ggcggcctgc tgtttctgat gtctgcgatc gctgcgacgt ttgccggaag gctcgccgat
      901 caatggatca atgcgggtgc gactgccacg gtggttcgca agggcacgct ggtcgcgggc
      961 cagattggca tcggcgtatg tctggtcctt actccgctga cccatggtgg cgtattcatc
     1021 gtcatgctcg cgatgacagg cgtgttcctg ggaattgggt gttgcagcac gtgggccgtc
     1081 acgcaaacct tggccggtcc gctcgccgca ggacgctgga ccggagtgca gaacttcgtt
     1141 ggcaacatgg caggatgggt agctccggtg ctcacgggat tcctggtcga tcgaacggga
     1201 cgctttgaac gggcattctt catcaccgca gcaatcgcat ggacaggcgc cctgagctgg
     1261 ggcctcctcg taggaccggt ggaacccgtg aactggaaac aactgtcttc gcctcgagaa
     1321 gccatctcgt aaatcgcttt gaaagggcgc ggcttccagc cgcgccgcaa ggcgccacaa
     1381 acgacgcggg ctttagcccc tgaggacaac cggcggtcag ctgttcgatt cattatgaga
     1441 tggcctttgg tagccgtgcc tactccgact ggatctgcgc cagccgccta cgcccgggcg
     1501 cgcgtaatac actgctctta tggcgcagga gatcgaaatc aaatttcgcg ttcgggattt
     1561 gcgcgctctt aatcgcaggc tgcaagcgtt gggattccgg ctggtcacgc gccgcactca
     1621 cgagatgaat accctctacg acctgccggg agaggttttg cgcaagcgac gccagctttt
     1681 gcggctgcga caatacggct cgcaatggac gctgacccac aagcgcggca ccacgcgtgg
     1741 acaccacagc agccgcgtcg aactcgagac ctccgtccgt gatggaaagc agatggatgc
     1801 aattctgggc gcgctggggt attccccgtc gttccgttac gaaaagttcc gcgctgagtg
     1861 ggcggatggc aagggacaag ttgtcgtaga cgaaacgccc atcggtaatt tttgtgagat
     1921 cgaagggccg ccgcgctgga ttgacgcaac cgcgaaaaag cttggagtga agtcaactgt
     1981 ctatatcacg aagaactatg cctcgctgtt cctggagtgg aagcatgaga cagggagcgc
     2041 ggcagaggag atgacattca aggcagtgaa gcgatgagac gatcgacctg cttttttcgc
     2101 cccccattcc ggagagggaa aaagtcaaga cctcgaccgc atacggcatg ccccggaaat
     2161 ggtgcggttt tctcgtaacg agctgattcc acgtatgctt tcccgcctga aatgctgtgg
     2221 aagaaataat gtatatctag tctgcaatca acaatataga aatggaagtt aaatcgattt
     2281 aggaagccgc tggcgcgagg gcaggtcgct ctcaccctgc ggttcgaccg tggcgccgat
     2341 ctcgcctctg ctgccgcgag gagcgcagcg cgaggcatcg cagccagcaa gccgcctggc
     2401 attttggcca actcgcgatc gatgcttaaa cggccgggag gcctgccaga gtgcgcagtg
     2461 ttcgctgctg ctcgtcagcc aggtatcgct tcgcgaagat gaacaacgtg ccctgcgagt
     2521 agaacataaa cacacgcgcg tcctcgccct tgggcgagac ctccgtccag tgtttattcc
     2581 aggtccggtt ctcccctgtg acctggaatc cgtctgcggt gaggttggcg cggtatctct
     2641 tcccttccag tccgaggcgg cgatagtagt tgcgatagta gcgccaaaac atggcgggaa
     2701 agatccttcc caggccggcc cacatccacc acacgccgat cgcgccgagc aggattgggg
     2761 acaagccccg ctctctctcg actagcgcca cggcgagcag gcatgctccg ccgacgaacc
     2821 ataccccatc ccggttgaaa ttacgattga gccggcaatg cagaacggaa gccgcggcgt
     2881 agtcgtcggc tgagatttcg tactcaaatt ccatgatcca aggatcgacg tctagcgcag
     2941 gatggctacg ccccgtggca cttcttgtat ttcttccccg acccgcaggg gcaaggatcg
     3001 ttgcgcccga ctttttcctg tgaacggacg acttgctgta ccggcgctgc gtcgccggcg
     3061 ccggccatgc gcgcctgctc cagttcgcgc cgcttgcggc gcatgaaggc ttcttccagc
     3121 tcgtcggccg aggtcgagac caggtgtggc ggacggcgac cgttgccgtc gccgcctctc
     3181 ggaaccggaa cgcccgcatc cgggctttga ccggccgggc cgcccggggg aggcgctgaa
     3241 ggtccgcccg ggccctcctc ccgcggccgc tccagaatct gcatcaggta gagataccgg
     3301 accgtatctt cctggaagcg ctgcatcatc gcttcgaaca tctcgaacga ttctttcttg
     3361 tactcgacca gcggatcgtg ctggccgtac ccgcgcagcc cgatgccttc tttcaggtga
     3421 tccatgctga gcaggtgatc tttccattgc tgatcgatca cgctcagcat gatcatgcgc
     3481 tcgtgatggc gcatggcctc ggggccaatg agcttctctt tggcttcgta gcgttctttc
     3541 agtttcgcga agatggcgtc gccgagttcc ccacgattca gcgtctccgg ctttatgcct
     3601 tcggcgagga tgtcgacgcc aaagcgggtg aagattgcgt ctttcaggcc cttgacgttc
     3661 cagtcgtcga cgtgcgcctt ctcggggcag tattgctcaa gaagatcgcc caaaatcgcg
     3721 gatacgtagt cctcgaggat cagatctttc tgatccatgc cctcgagcaa tcggcggcgc
     3781 aggccataga cggcctcgcg ctgcttgttc atcacgtcgt cgtattccag cagatgtttg
     3841 cgcgactcga agtgctggcc ttcgaccgcc ttctgcgccg tctcgacgcg gcgggtcatc
     3901 atcttcgatt cgatgggcac gccttcttcc atgcccagcc gctgcagcat cttcgacacc
     3961 cattccttgg gaatgaagat gcgcatcaga tcatcttcaa gcgcgagata gaaccgggat
     4021 gatccgggat cgccctgacg gccggcacgc ccccgcagct gattgtcgat gcgccgcgac
     4081 tcgtggcgtt ccgtgcccag aatgtgcagg ccgccgacgg acgtgacctc gtcgtgctct
     4141 ttgtcagtct gctctttata gcgattgagg gcttcggtcc acttgtcagc gggaaccagg
     4201 tactcgttgc cgttgtagta gaaaatcgaa ctctcgccgt cttcctgcgg cccttcgatc
     4261 ttgccctggg ccacgcgtaa ctgctgcgcg attcctttct ttaccatttc ctgcttggcc
     4321 atgaactcga agttgccgcc cagcagaatg tcggtaccac gacccgccat gttggtcgcg
     4381 attgtgacca tgcccttgcg tccggcttgg gccacaaatt ctgcttctcg ctcgtggaac
     4441 ttggcgttca aaacgacgtg cttgatggcc tttttcttga ggaggtccga aagccgctcg
     4501 gacttctcga ctgacgtcgt gcctaccaac acgggctgtc cttttgccgc gagttgctgg
     4561 atttcatctg ccaccgcgaa atacttttct ttttcggtgc ggaagatgac gtcaggattc
     4621 tcgatgcgca gcagtacctt gttggtcgga atcacgacca cgtccagctt gtagatcttg
     4681 tcgaactcgg tggcttccgt ctcagccgta cccgtcatgc cggccagctt cttgaacatg
     4741 cggaagtagt tctggaaggt gatggtggcc agcgtctggt tctcgcgctc gatcttgacg
     4801 ttttctttgg cttccaccgc ctggtgcagg ccgtcggacc agcgccgccc cggcatcatg
     4861 cgcccggtga actcatcgac gatgatgact tcgccgtcct taatgacgta ctcgacgtcc
     4921 ttgcgataga gcgcgtgcgc ctttactgcc gtctccacgt gatgcttcca gccccagttc
     4981 tcgggatcag cgatgttgcc gatgccgagc aggccctcga ccttctccca gccttcgtcg
     5041 gtcacggtaa tgttgcgatg cttctcgtcg accacatagt cgcccgtgta ttgcggtggt
     5101 tcgcccggag gtccctcgat ctcttcgcct ttttcgagtt tcgggatgat
//
LOCUS       DASPUL010000345         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1706331, whole genome shotgun sequence.
ACCESSION   DASPUL010000345 DASPUL010000000
VERSION     DASPUL010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706331"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VEJ00_08330"
     CDS             <1..867
                     /locus_tag="VEJ00_08330"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXX01205.1"
                     /translation="SRGNPYGKLEKKRADWTKVAESERKIKVLDGKNGADTLYFVDSI
                     TSYDDRMQSIGQATARILTQIGTNFGILGTAEKDSGHDVRRFGEETLFMELRDHNTEA
                     IKESGVQKIVTADPHAFNALRHDYKDVPPVEHISQVLAREVKSGNIQFNRVEDGKTYA
                     YHDPCYLGRHNQVYDDPRSVLDAIPGLKRVEMKRSRDRSFCCGGGGLMLFYEPKEEQR
                     MGVLRVKMAAEAGANVIVTGCPFCMVNIEDAIKVAGMEGKMSVIDLTELVDQNMVRES
                     RPEVCEAVTCGG"
     gene            870..1658
                     /locus_tag="VEJ00_08335"
     CDS             870..1658
                     /locus_tag="VEJ00_08335"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HXX01206.1"
                     /translation="MEILVCARRVPDTSENEITLNNGGNDIERDELVYSINEADNYAV
                     EEALQIRDRVGGNVTVITVGGEDDEEILRRELAMGANQAVLVSDEAFTGADGKGIATI
                     LKDFVKKGHYDLILTGVQADDGAAQVGGMLAAILDYPFASLVCSITAEDGKLRVEREI
                     EGGNREINEIDLPCVLSIQTGINEPRYVGMRGIRQVASVPIPTLGAADLGSDSNAVGE
                     AAAKVKRVDYFVPAAGKGAQILSGSREENADKVLELVAARGGLK"
     gene            1658..2638
                     /locus_tag="VEJ00_08340"
     CDS             1658..2638
                     /locus_tag="VEJ00_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXX01207.1"
                     /translation="MARIFAYIAQKAGMVDDSGTELLAAAKKFDAAASPTVILTGWGP
                     ELEAACNSLCSCFAEVWKVANQALAYPNAELVRQALLSVVPPNSILLVPHDHFGIDLA
                     PGLSIKLNAAFVSDVVAIDGVEGNVLKLVREEFGGQVSAHVHCDVSTGAVITIRPGAF
                     KAENVAVSGVVFDKSSAVGVLTARRRYVETIVAEAGDVDITKHSVLVSIGRGIQEKDN
                     VGVAEELAEALGAAVSCSRPVVDAKWMEKSRQVGSSGKTVKPKVYIACGISGAFQHLA
                     GVKGSPFLIAINKNPKAPIFQVADVGIVDDILEFLPELTNKARELRGVAK"
BASE COUNT          628 a          751 c          882 g          512 t
ORIGIN      
        1 agccgcggca atccctacgg caagttggaa aagaaaagag ctgattggac caaggttgcc
       61 gaatcggaac gcaagatcaa ggtgctcgat ggcaagaacg gtgccgacac tctctacttt
      121 gtcgacagca tcacttccta tgacgaccgg atgcagtcga tcgggcaagc cacagcccga
      181 atcctcaccc agatcggaac gaacttcgga attctcggca ccgcagaaaa agacagtggc
      241 catgatgtgc ggcggttcgg cgaggaaacg ctgttcatgg agctgcgcga tcacaacacc
      301 gaagcgatca aggagtccgg agtccagaag atcgttaccg ctgacccaca tgcgttcaat
      361 gccctcagac acgactacaa ggatgttccg ccggttgagc acatcagcca ggtgcttgcg
      421 cgggaagtaa agtccggcaa catccagttc aaccgagtcg aggatggaaa aacatacgcc
      481 tatcacgacc cctgctacct gggccgccac aaccaggtct atgacgatcc ccgctccgtg
      541 ctcgacgcca tcccgggatt aaagcgagtc gaaatgaagc ggtcgcgtga ccggtcgttc
      601 tgctgcggtg gcggcgggct gatgttgttc tacgaaccca aggaagaaca gaggatgggc
      661 gtgctccggg tcaagatggc ggcggaagcc ggggcgaacg tcatcgtgac cggttgcccg
      721 ttctgcatgg tgaatataga ggacgccatc aaggtcgcgg gcatggaagg aaaaatgtcg
      781 gtgatcgacc tgacggaact tgtggaccag aacatggtgc gggagagccg tccggaggtc
      841 tgtgaagcag taacttgcgg aggttaaccg tggagatcct cgtttgcgcg cgaagagtgc
      901 cggacacctc ggagaacgag atcacgctca ataacggcgg gaacgacatt gagcgcgatg
      961 aactcgtgta ctccatcaac gaagcggaca actatgcggt cgaagaggcg ctgcagattc
     1021 gtgatcgagt cgggggcaac gtcaccgtga tcaccgttgg tggtgaagac gacgaagaga
     1081 tcctgcgccg ggagctggcc atgggagcca accaggccgt gctcgtctcg gacgaggcct
     1141 tcacgggagc ggacggcaag ggcattgcaa caatcctcaa ggattttgtg aagaaaggtc
     1201 actatgacct gattctgact ggagttcagg ccgatgacgg cgcagcgcag gtgggcggaa
     1261 tgctggccgc aatactcgac tatccgttcg cctcgctggt ctgttcgatc acggctgagg
     1321 atggaaagct cagggtggaa cgcgagattg aaggcggcaa tcgcgagatc aacgagatcg
     1381 atctgccctg cgttctgtcg attcaaacgg gcatcaacga gccgcgttat gtcgggatgc
     1441 ggggcatccg ccaggtggct tcggtcccga tcccgacgct gggcgcggca gacctcggca
     1501 gcgacagcaa cgcagttgga gaagctgcag cgaaggtcaa acgcgtggac tatttcgtgc
     1561 ccgcggccgg caagggagca cagatcctca gcggcagccg cgaagaaaat gccgacaagg
     1621 tcctggagtt ggtcgcagca agaggagggc tgaaataatg gctcgcatct ttgcatatat
     1681 cgcgcagaag gctgggatgg ttgacgattc ggggaccgag ctgctagccg ccgcgaagaa
     1741 gttcgacgcc gctgcttccc caactgtgat tctcaccggc tgggggccgg aactcgaggc
     1801 cgcatgtaac tctctttgct cttgctttgc tgaggtctgg aaagttgcca accaagccct
     1861 cgcctatccc aacgcggagt tggtgcgcca ggccttgttg agcgttgtac cgccgaacag
     1921 cattctgctc gttccgcatg atcacttcgg aatcgacctc gcgccggggc tatccatcaa
     1981 gctgaacgct gcttttgtct ctgacgtggt agcgatcgat ggagtcgagg gaaacgtcct
     2041 caaactggtt cgcgaggaat ttggcgggca agtcagcgcg cacgtgcact gcgatgtttc
     2101 gacaggagct gtgatcacga ttcgacccgg agcgttcaaa gcggaaaatg ttgccgtttc
     2161 cggtgtggtc ttcgacaagt cgtcggcggt tggcgtcttg acggcgcggc ggcggtatgt
     2221 cgagaccatc gtcgccgagg cgggcgatgt cgacatcacc aagcacagcg tgctggtctc
     2281 catcggtcgc ggcattcagg agaaggacaa cgttggcgtt gccgaagaac tcgctgaagc
     2341 gctgggcgcg gctgtgagct gctcgcggcc tgtggtcgat gccaaatgga tggagaagtc
     2401 gcgccaggtg ggctcgtcgg gaaagacggt gaagccgaag gtctacatcg cctgcggcat
     2461 cagcggtgcg ttccagcacc tggccggcgt caagggaagc ccgttcctga tcgcgatcaa
     2521 caagaatccc aaggcgccga tcttccaggt ggccgatgtc gggattgtcg acgacattct
     2581 cgagttcctg cccgaactaa ccaacaaggc ccgcgaactg cggggcgttg ccaagtgagg
     2641 taggttaatg cttgcctaca aaaccttaaa gttgagcttg aagtccctga aagagttcgc
     2701 caaaaagcgc ctgcccgacg aaaccctgat cgaactcgac gaaaaagacg agtgcccgct
     2761 cgacatcgtg cgc
//
LOCUS       DASPUL010000346         5052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_893822, whole genome shotgun sequence.
ACCESSION   DASPUL010000346 DASPUL010000000
VERSION     DASPUL010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893822"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /gene="hutI"
                     /locus_tag="VEJ00_08345"
     CDS             <1..819
                     /gene="hutI"
                     /locus_tag="VEJ00_08345"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HXX01208.1"
                     /translation="TTVEAKSGYGLTVESELKSLEAIREAAARWPGTVVPTLLGAHVV
                     PKEFKGRAEEYVKIVCNQMIPKAAKRKLAQFVDVFCDKGAFTAEETEKIFQSAASHGL
                     SVRAHMGQLSETLLWPFLRFHPASFDHMDHVNEDDIPQLAKRDTVATLVPGANYFLGL
                     KEYPDARRLIAAGVPVALATDYNPGTSPTLSMPMAMSLACTHMKMSPAEAIAAATING
                     AWALRLAERKGSIEPGKDADLAIFDVSDYREIPYWFGDNRCSMTVCNGGIAFRR"
     gene            complement(864..3095)
                     /locus_tag="VEJ00_08350"
     CDS             complement(864..3095)
                     /locus_tag="VEJ00_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXX01209.1"
                     /translation="MNSLHRALIPPLLACGAFLLPAMSIAQDPSPPAQAPENRPRVGL
                     ALSGGGALGLAEIGVLQWLEENHIPVDRIAGTSMGSIIGAMYATGMSPAEIQKFAEGI
                     DWDEAFLPEPTYAQLSYRRKQDRRDFLINAPLGLKHGLSGPNGINPGQGVGLWLDRIA
                     FPESDVASFDDLPIPFRCVATDMQSGDAVVLRDGPLAQAVRASMAIPGVFTPVEINGR
                     ILADGGMVQNIPVETVRAMDADKVLAVQLRTPPGDRAELETITGVLTRAVDVMITQNE
                     RRSLALADASISVDMTGFSVSDYSRLKDLVQLGYKSAAMEAAALLPYAIQDSAEWESY
                     LAARAARRHPRPDRIRAIEVTGADPESDRRIEHQLAKDVRGPLNLRTLDSQLTRIAGE
                     GRFDRLGYEGFSQNGVPALRVTTHEKSYGPPFVDLAVNVDGSGVAAFDFSAGARVTFM
                     DIAHRGGEWRNDLLFGSSNLAATEYFQPVAGSRFFVAPYAFASKFARNSFSGLTRVAV
                     FGDERAGGGFDLGYDSGRRSEFRVGYEIFSGKLSPLVGQAGLPIVSGSTGEFRARYVW
                     DGQDSPAVPSTGTRVIATLSRVLESPGLAHPIGQLDVQTSTFVPVVPKTSLFLAASGG
                     TTFRGSAGPFQEFALGGPFRLGAYLPQQFLGNHYAYASLGFRREVYRLPQPVGNKIYW
                     GAWYEAGSAFGTTAGDPGPVVVRGTFNLGVIAETIVGPIALAGSVSPTGQSRANFSIG
                     RIF"
     gene            complement(3217..>5052)
                     /gene="argS"
                     /locus_tag="VEJ00_08355"
     CDS             complement(3217..>5052)
                     /gene="argS"
                     /locus_tag="VEJ00_08355"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXX01210.1"
                     /translation="RAGIGPVEGIADAQVTPPGYLNVRIDRAWLASALAADRTPATDI
                     PTGKILVEHSSINPNKAAHIGHLRNAILGDTFVRLLRHAGREVDVQNYIDNTGVQVAD
                     VVVGFVHLEKKSPSEIEALARQPRFDYYCWDLYARVSQWYERDKQNQQVRLQTLHAIE
                     DAGSETAAVADLISTAVLRRHLETMDRLDIEYDFLPRESEILHLHFWDAAFTKLKESG
                     VLTFESEGKNKGCWVMRRAGTKSETAKNAEKAAKDAKKTDGAEEPISEEDQKVIVRSN
                     GTVGYVGKDIAYHMWKFGLLGRDFGYRRFYRYPNQHDCWISTDPNQPDAEKDHPHFGD
                     VAEIYNVIDARQSEAQSTVIEALRGLGHDEAADHYTHFSYEMVALTPRCAAELGYTLS
                     EEDKARSYIEVSGRKGFGVKADDLLDALIAAAKQEVDSRHPQATDEERTRIATQIAIG
                     ALRYFMLKYTKSSVIAFDFKDALSFEGETGPYAQYAVVRATNIFRKGGLDPENFAAAT
                     KLPADDLQTYLTAETGTEIWELWLTASKTSYVIEQCIGTTEPAYLAKHVFQLAQLFNT
                     FYHRHPILTESDEKRKAFLLATVAVVRRELIRALAVMGIAVPPVM"
BASE COUNT          963 a         1517 c         1587 g          985 t
ORIGIN      
        1 accacggtcg aagccaaatc cggctatggg ctgacggtcg agtcggagct caagtcgctc
       61 gaagcaatcc gcgaggccgc tgcccgttgg ccgggaacag tcgtccccac gctgctcggc
      121 gcacatgtgg tgccgaagga attcaaggga cgcgcggagg aatacgtcaa gatcgtctgc
      181 aaccagatga ttccgaaagc agcgaaacgg aagctcgcac agtttgtgga tgttttctgc
      241 gacaaaggag ccttcactgc ggaagaaacg gaaaagatct ttcagtcggc ggcctcgcac
      301 ggcctgagcg tgcgcgcaca catgggtcag ctttcggaaa cgctgctgtg gccgtttctg
      361 cgcttccatc ccgcatcgtt cgatcacatg gaccacgtga acgaagacga catcccgcag
      421 ctggccaagc gggacacggt cgccacgctg gttccgggcg cgaactattt cctcgggctg
      481 aaagagtatc cggatgcgcg acggctgatc gccgctggtg tgcccgtggc cttggcgacg
      541 gactacaacc cgggaacgtc tccgacgctg agcatgccca tggcgatgtc actggcctgc
      601 acacacatga agatgtcgcc ggcggaggct atcgcggcgg ccacgatcaa tggcgcatgg
      661 gcactccggc tggcagaacg aaaaggcagc atcgagccgg gtaaagatgc cgacctcgcg
      721 attttcgatg tgagcgacta tcgcgagatc ccctactggt tcggagacaa ccgctgctcc
      781 atgaccgtgt gcaacggcgg gattgcgttc cgcagataaa gccggccgcg cacgtcattt
      841 cctcagacga ttgctcccct tcctcaaaag atccgcccga tcgaaaaatt cgcccggctc
      901 tggcccgtgg ggctgacgct gccggccagc gcgatcggtc cgacgattgt ttctgcgatg
      961 actcccaggt tgaacgtgcc acgcaccacg actggcccgg gatcgccggc ggtagtgccg
     1021 aaggcacttc cggcttcgta ccacgctccc cagtagattt tgtttccgac cggctggggc
     1081 aggcggtaaa cctcgcggcg gaagcccagg gaggcataag cgtagtggtt gcccaggaat
     1141 tgctgtggca gataggctcc caggcggaac ggcccgccga gtgcaaactc ctggaacggc
     1201 ccggccgagc cgcgaaatgt cgttccgccc gacgctgcga ggaacaggga cgttttgggc
     1261 acgaccggaa caaatgtgga agtctgtaca tcgagctggc ctatcggatg cgcgaggccc
     1321 gggctctcca gcacacgcga gagcgtggca atcactcgcg tgccggtgct ggggacggcg
     1381 gggctgtcct ggccatccca tacatagcgc gcgcggaatt ctccggtgct gccgctgacg
     1441 atgggcaggc ccgcttgccc gacgagcggg ctcagcttgc cgctgaagat ttcgtagcca
     1501 acgcgaaact cgcttcgccg cccggagtcg tatcccagat cgaagccacc cccggcgcgc
     1561 tcgtcgccga acacagcaac ccgcgtcaac ccgctgaacg agttccgggc gaacttcgag
     1621 gcgaaggcat atggggccac aaagaaccgt gaccccgcga ccggctggaa gtactcggtt
     1681 gcagcgaggt tgctcgaacc gaagagcaga tcgttgcgcc attcgccgcc acggtgggct
     1741 atatccatga acgttacgcg cgcgcccgcg gagaaatcaa acgcggctac gcccgagccg
     1801 tcgacattca ccgccagatc gacgaacggc ggcccatacg atttttcgtg ggtggtaacg
     1861 cgcagcgccg gcacgccatt ctgagaaaaa ccctcgtagc cgagccggtc gaagcggcct
     1921 tctccggcaa tgcgcgtcag ttgtgaatcg agagtcctga gattgagtgg cccgcgcacg
     1981 tcctttgcaa gttgatgctc gattctgcga tcggactctg ggtcagcgcc agtgacttca
     2041 atcgcccgaa tcctgtccgg tcggggatgt ctgcgcgcgg cgcgtgccgc aaggtagctc
     2101 tcccactcgg cagagtcctg aattgcgtat ggcaacaacg cggcagcctc catggcagcg
     2161 ctcttgtatc ccaactgcac gagatccttc aagcgcgagt agtcgctcac cgagaatccg
     2221 gtcatgtcga cgctgatgct ggcgtcggca agcgccaggg aacggcgttc gttctgcgtg
     2281 atcatgacat ccacagcccg ggtgaggact cccgtgatgg tctccagttc agcgcggtcc
     2341 ccaggcggag tgcgcaactg cacggcgagg accttgtcgg catccatggc tcggacggtc
     2401 tcgaccggga tgttctgcac catgcctcca tcggccaata tgcggccgtt gatctcgact
     2461 ggagtgaaga ctccgggaat tgccatcgaa gcgcgaaccg cctgggccag cggaccgtcg
     2521 cgcaacacga cggcatcgcc actctgcatg tcagtggcga cacagcggaa aggaatcgga
     2581 aggtcgtcaa agctggcgac atccgactcc ggaaacgcga tgcggtccag ccacaggccg
     2641 acgccctggc cagggttgat gccgttgggg ccactgagcc cgtgtttcag gcccagagga
     2701 gcattgatca ggaagtcgcg gcggtcctgc ttccggcgat aggagagttg tgcataggtt
     2761 ggctcgggga gaaacgcctc gtcccaatcg attccttcgg cgaacttctg gatttcggcc
     2821 ggcgacatgc ctgtggcata catagcgccg atgatcgatc ccatgctggt tccggcgatg
     2881 cgatcgaccg gaatgtggtt ttcctccagc cactggagga ctccgatttc agccaggccg
     2941 agcgcgcccc cgccgctgag cgccagaccg acgcgagggc ggttctcggg agcctgcgca
     3001 ggaggcgatg ggtcctgcgc gatcgacatg gcaggcagca agaaagctcc gcaagcgagc
     3061 agtggcggga tgagtgcgcg atgcaatgag ttcatcgttt actcctggag actttgggaa
     3121 cgcggttcct acatctgaac ccacggcgag agaaaaggtt agcaggtccc cgcggtgacg
     3181 agtcagcgca cttgggcaat tgcatccccg agccgcttac atgaccggcg gcaccgcgat
     3241 tcccatgacc gcgagcgcgc ggatgagttc gcggcgaacc accgcaactg tcgcgagcag
     3301 gaacgccttc cgcttctcat ctgattccgt aaggatggga tgtcggtggt agaaggtgtt
     3361 gaacagctgg gccaactgga aaacatgttt ggccaggtaa gcgggctcgg ttgtcccgat
     3421 gcattgctca atcacatacg atgttttcga ggccgtcagc caaagttccc agatttccgt
     3481 accggtttcc gcggtcagat atgtctgcaa gtcgtcggcc gggagtttgg tagccgctgc
     3541 aaagttctcc gggtccagcc cgccttttct gaagatgttg gttgcgcgga ctacggcgta
     3601 ttgcgcgtag ggtcccgtct cgccttcgaa gctcaacgcg tccttgaagt cgaacgcaat
     3661 cactgacgac ttcgtgtact tgagcatgaa atagcgcagg gcgccgatcg cgatctgggt
     3721 ggcaatgcgg gtgcgttctt cgtcggtggc ttgcggatga cgcgagtcga cttcttgctt
     3781 ggcagcggcg atcagcgcgt cgagcaggtc gtcggccttc acgccgaagc ctttgcgacc
     3841 gctgacctca atgtaggagc gggccttgtc ttcctcgctc agcgtgtagc caagttcagc
     3901 ggcacagcgc ggggtcagcg ccaccatttc ataggaaaaa tgggtgtagt gatcggcggc
     3961 ttcgtcgtgg ccgaggccgc gcaaggcttc gatcaccgtg ctctgcgcct ccgattggcg
     4021 ggcgtcgatg acgttgtaga tttcggcgac gtcgccgaag tggggatggt ctttctctgc
     4081 atctggttga tttgggtcgg tggagatcca gcagtcgtgc tggttgggat agcggtagaa
     4141 cctgcggtat ccgaaatcgc gtcccagcag cccgaacttc cacatgtgat aggcaatgtc
     4201 tttgcccacg tagccgacgg tgccattgga gcggacgatg actttctggt cttcctcgga
     4261 gatcggctcc tcggctccat cggtcttctt tgcgtccttt gcggccttct ctgcgttctt
     4321 tgcggtttca gatttcgtcc ccgcccgccg catcacccag caacccttgt tcttgccctc
     4381 actctcgaac gtgagcacgc cgctctcttt cagcttggta aaggccgcat cccagaagtg
     4441 caggtgcaat atctcgcttt cgcgcggaag aaagtcatat tcaatatcga gccggtccat
     4501 ggtctcgagg tggcggcgca agaccgccgt cgagatcagg tcagcgacag ccgcggtttc
     4561 acttcctgca tcttcgatgg catggagcgt ctgcagacgc acctgctggt tctgtttgtc
     4621 gcgctcgtac cattgcgaaa cgcgagcgta gagatcccag cagtagtagt cgaagcgagg
     4681 ctggcgcgcg agcgcctcaa tctcggaggg cgatttcttt tccagatgca cgaatcccac
     4741 aaccacgtca gcgacctgga cgccggtgtt gtcgatgtag ttctggacgt cgacttccct
     4801 gcccgcatgg cgcaacaggc gcacgaaggt gtcgccgaga atcgcgttgc gcagatgtcc
     4861 gatgtgggca gccttatttg gattaatgct ggagtgctcg accagaatct tgcccgtggg
     4921 aatatcggtt gcaggtgtcc gatcagcggc aagcgcggag gcgagccacg ctcgatcgat
     4981 gcggacgttc agataaccgg gaggggtaac ctgggcatcc gcaatgccct cgaccggtcc
     5041 gatcccggcg cg
//
LOCUS       DASPUL010000347         3180 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1564617, whole genome shotgun sequence.
ACCESSION   DASPUL010000347 DASPUL010000000
VERSION     DASPUL010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1564617"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3031
                     /locus_tag="VEJ00_08360"
     CDS             <1..3031
                     /locus_tag="VEJ00_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01211.1"
                     /translation="KSVHANGMRSIDNNFQVNGLQVNDIDGGAQGVPIPNPDTIQEFK
                     VQTGQYDASFGRNAGASVNLTTRSGANQFHGNVFEFFRNTVLNANDYFVKQTGQPRGV
                     LDQNQFGGTLGGPIKRDKAFFFGSYQGTRQRNGIALGCFSTMVLPPLTNDRSALGIAQ
                     IFDGQTGALQSEAIQDGFPPFLFPAIDATSPNGNGTPNPYNINPVALALLQMKLPDGS
                     FLIPNPQTPSGLTILQNPCTFNEDQYLADVDYTQNQRSAFFARYFIANSHQTMTFPSA
                     GIFNTNATEGSPSFQPQRFQALALGHTFTISPTLVNEFRAGFHRTTSQSQQTNPFTFS
                     SVGANVPAFYNDLPTFDIGNGSCCTAGGGAPSALYQGSWDIVDSLAWTHGKHSMRFGG
                     GVTRSYMNQRDFRSNGIEDFITFPDFLLGLDAADNGLAIANAITHGAFPAFSDLIVSI
                     DYVGISDRNERTWEGSAYFQDDIKVTPRFTLNAGFRYERIGELGDTTGRNGNFNPALY
                     TVPPSPAGCPPTCPGILNGYTVPSNFQGTIPAGVTKTGNNLGIPGLGQNAWAPRLGFA
                     WQVLPNTDRLVVRSGYGIYYTRPVGAAILETISSAPFGLFQVCERACNGGASEQNPFQ
                     PAPPVSAFPVFQPYSQTTNQSVLLLGQDFRPPIIQEYSLGVQSRLAHDFLLELGFVGS
                     RSTRVIRQRGLNQALSASPQNPVNGATDNEFANINQRLPLIGFASDAASVNQIESNGA
                     SWYNGLQASLTKRYSNGLQFLASYTWSKEMDTNGVDPEWNSAGGTASLGNQSPAPRTQ
                     YGPGGFNRNQRFVFSSVYLLPGPKDLSGLKGRVLGGWSISGVATIQSGQYLTLTGENS
                     FNDFGITNDRVELAPGCTSAQLANSGSVKSRLNNYFNGNCINRPDLTMPLNSVTNSAS
                     WPVIDPSPGGLGTDFGNSAVGAVTGPGQHNYDFTLEKMFSFRESMNVQFRADFFNLFN
                     HAQFANPEVSTAEATFGQIISTTVNPRIVQFALKVNF"
BASE COUNT          692 a          968 c          865 g          655 t
ORIGIN      
        1 caagagcgtt catgccaacg gcatgcgctc gatcgacaac aactttcagg tgaacggtct
       61 tcaggtaaat gacatcgatg gaggagcaca gggtgttcca attcccaacc ctgacacaat
      121 ccaggaattc aaggtgcaga ccgggcaata cgacgccagt tttggaagaa atgcgggagc
      181 cagcgtaaac ctgaccacga gatccggagc gaatcagttt cacggcaacg tgtttgagtt
      241 cttccgcaac acggtcttga atgcgaacga ctattttgtc aagcaaacgg gtcaaccgag
      301 gggtgtgctc gaccagaatc agtttggcgg tacgcttggt ggtccgatca agagagacaa
      361 ggcgttcttc ttcgggtcgt accagggcac gcggcagcga aacggcattg cacttggctg
      421 cttctccacc atggttctcc cgccgctcac caatgatcga tcggcgctgg gcattgcgca
      481 gatattcgac gggcaaaccg gtgctctgca aagcgaagcc attcaggacg ggtttccgcc
      541 attcctgttc ccggcgatcg acgcgacctc gccgaatgga aacggcacgc ccaatcccta
      601 caacatcaac cccgtggccc tcgcgttgct tcaaatgaag ctgcccgatg gaagttttct
      661 gattcccaat ccgcaaacac ccagcggcct taccatcttg caaaatccct gcaccttcaa
      721 cgaagaccaa tacctggccg acgtagacta cacgcaaaat caacgcagcg ctttctttgc
      781 tcgctacttc atcgccaaca gccaccagac gatgacgttt ccctccgcgg gaatttttaa
      841 taccaacgcc acggaaggat ctccgagctt tcagccgcag cgcttccagg cactggccct
      901 cgggcacaca ttcacgatca gcccgacgct ggttaacgaa tttcgcgccg gatttcatcg
      961 aacgaccagc caatcgcagc agacgaatcc cttcacattt tccagcgttg gcgcgaatgt
     1021 gcctgctttt tacaatgacc ttcctacgtt cgatattggc aatggcagtt gctgtacggc
     1081 aggagggggc gctccttcgg cgctgtacca gggaagctgg gacatcgtcg actctttggc
     1141 ctggactcat gggaagcaca gtatgcgatt cggcggcggg gtcacccgca gctacatgaa
     1201 ccaacgcgat ttccgctcca atgggatcga ggactttatt actttccctg atttcctgct
     1261 gggtctcgac gccgctgaca atggtctggc gattgcaaac gcgattaccc atggagcgtt
     1321 tccggcgttc agcgacctta ttgtgtcgat tgactacgtc ggaatctcgg accgcaacga
     1381 acgcacctgg gaagggtccg cgtactttca ggacgacatc aaggttacgc ctcggttcac
     1441 actcaacgcc ggatttcgct acgagcgcat tggagaactc ggggacacga cgggccgcaa
     1501 tggcaacttc aatcctgcgc tctataccgt cccgccttct ccggcgggct gccctcccac
     1561 ttgcccgggg attctgaatg gctacacagt gccttcgaac ttccagggta cgattcctgc
     1621 cggcgtcacg aaaaccggaa acaacttggg tatacccggc cttggacaaa atgcctgggc
     1681 gccgcgacta ggttttgcct ggcaagtgct gccgaatacg gaccgcctgg tggtgcggtc
     1741 gggctacggc atttactaca cgcggccggt gggcgcggcc attctcgaga ccatatcatc
     1801 cgcgccgttc ggccttttcc aggtgtgcga aagagcctgc aatggaggtg ccagcgaaca
     1861 gaatccgttt caacctgctc ccccggtctc ggcgttcccg gtgtttcaac cgtattcgca
     1921 gaccaccaat caaagtgtcc tccttctggg ccaggatttt cgacctccca tcatccagga
     1981 gtacagcctc ggagtgcagt cccggctggc ccacgacttt ctcctcgagc tcggatttgt
     2041 cggatcgcgc agcaccaggg tcattcgcca gcgcggcttg aaccaggcct tgtcggccag
     2101 cccgcaaaac ccagtgaacg gggcaacaga caacgagttc gccaacatca accagcgctt
     2161 gccgctgatc ggcttcgctt ccgacgccgc ctccgtgaac cagatcgaat cgaacggcgc
     2221 ctcctggtac aacggcctgc aagcgagctt gaccaagcga tacagcaacg gacttcagtt
     2281 cctggcgtcc tatacttggt ccaaagagat ggacacgaac ggcgtcgacc ccgagtggaa
     2341 cagcgcgggc ggcaccgcca gtctgggaaa tcagtctccg gctccacgca cgcaatatgg
     2401 accggggggc ttcaaccgaa accagcgatt cgtcttcagc tccgtctatt tgctgccggg
     2461 gcccaaagat ctttccggct tgaaagggcg ggtgctggga ggctggtcga tttccggcgt
     2521 cgccacgatt cagtcggggc aatacttgac tcttacaggt gagaacagtt tcaacgactt
     2581 cggcatcacg aacgatcggg tggagttggc tcccggctgc accagcgccc aactggcaaa
     2641 ctcgggctcg gtaaagagcc ggctgaacaa ctacttcaat ggcaactgca ttaatcgccc
     2701 ggatctgacc atgccgctga actcggtcac gaactccgct tcctggccgg ttatcgatcc
     2761 ttcgccggga ggcctgggaa cagattttgg caacagcgca gtcggggccg taacagggcc
     2821 gggacagcac aactacgact ttacgctgga aaagatgttc tctttccgcg agagtatgaa
     2881 cgtccagttc agggcggatt tcttcaacct attcaatcat gcgcaatttg cgaatccgga
     2941 ggtcagcacg gccgaggcaa ccttcggcca aattatttcg acgacggtca atccgcgaat
     3001 cgtgcagttc gcgctaaagg tgaacttcta gacatgcgca aacaaggctg ggccactcgc
     3061 cgatctctgg cagctctggc agtgctgctt tgcgcgggtt ggtcggccgc ggcaagggca
     3121 tctgactggc cgccgcccac ggacgaagcc atcgttacgc agcaccagat tacgctgaat
//
LOCUS       DASPUL010000348         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_60782, whole genome shotgun sequence.
ACCESSION   DASPUL010000348 DASPUL010000000
VERSION     DASPUL010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60782"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="VEJ00_08365"
     CDS             <1..671
                     /locus_tag="VEJ00_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXX01212.1"
                     /translation="NVPLDQALTELENDAKEVLPQGFTVDYAGESRQLRVEGSKFLGT
                     FLLSAILIYLVLAAQFESFRDPLIILAGSVPLAISGALVFSFLNFTTLNIYSEVGLIT
                     LVGLIAKNGILIVQFANHLQETGLTKLDAVIGAASTRLRPILMTTAATVCGHLPLVFA
                     TGPGAGARNSIGIMLVSGMIIGTGFTLFVVPSIYMLVAKTHFPIGDEDEQKIGLPHPA
                     EATV"
     gene            763..1854
                     /locus_tag="VEJ00_08370"
     CDS             763..1854
                     /locus_tag="VEJ00_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01213.1"
                     /translation="MRTFPRVRSVLLALMLLSASTALLAQIRISVAFGPPALPVYEQP
                     ICPGDGYIWTPGYWAWDPDAEDYYWVPGTWVLAPEVGYLWTPPWWGWEGGRFFFHEGW
                     WGPHVGFYGGINYGFGYFGAGFVGGRWDGGHFFYNREVTNINVVNIHNVYNEHINVTV
                     NHISYNGGPGGLGARPRPEEERAEHERHIGPVGAQTQHWEAARGNRELRASVNQGRPP
                     IAATGRPGEFSGHEVVNAREAGAPYHPPAERSGTAPRGGNAGFGSPVHPKELPPFERP
                     AAPNTGDTNRDRKYQQQQDKLFAKQEQERQNLQQKQEQEHQRMERQNAGQPQRQQMEQ
                     RHQQQTQQMQQKHVQQQQHLQDRQQPHRR"
     gene            complement(1965..>2748)
                     /locus_tag="VEJ00_08375"
     CDS             complement(1965..>2748)
                     /locus_tag="VEJ00_08375"
                     /inference="COORDINATES: protein motif:HMM:NF019625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HXX01214.1"
                     /translation="GTEVPAPGGESVDAHAPAVTTAEHVHQTMETFRLAFVQQGQSSA
                     WERVIALVVQPGVDFGSNTVFDYDPVKAQALSAALARHPGIVYEAHSTDYQTPSALAH
                     LVRDHFAILKVGPWLTFAYREALLALSGIEREMFKATGQRQISQVREALEAAMRRDPR
                     YWQSYYRGDEEEVRRDLTFGYSDRCRYYWNQPEVQKEVARLFENLAAGSIPPTLISQY
                     LPAEYEAVRAGELQASPERMIEYHIRRVLGVYANACSANQLS"
BASE COUNT          561 a          879 c          803 g          505 t
ORIGIN      
        1 ccaatgttcc tctcgaccag gcactgaccg agttggaaaa cgacgccaag gaagtcttgc
       61 cacagggatt cacggttgac tacgctggcg agtcccgcca gctccgagtc gagggcagca
      121 agttcctcgg cacgttcctg ctgtcggcaa tcctgattta tctcgttctc gctgcgcaat
      181 tcgagagctt ccgcgatccg ctcatcattc tcgccggatc ggtgcctctc gcgatctctg
      241 gcgccctggt gttctcgttt ctgaacttca ctacgctgaa catctacagc gaggtcggtt
      301 tgatcacgct ggttggactg attgccaaga acggaattct gattgtgcag ttcgccaacc
      361 acttgcagga aaccggcctg accaagctcg atgccgtgat cggcgcggcc agcacgcgcc
      421 tccgcccaat cctgatgacg accgctgcca ccgtctgcgg gcacctgccg ctcgtctttg
      481 ccacgggccc cggcgccggc gcccgcaaca gcatcggcat catgctggtc agcggcatga
      541 tcatcggcac cggcttcacg ctgtttgtgg tgccctcgat ctacatgctg gtcgcaaaga
      601 cgcacttccc gatcggggat gaagacgagc agaagatcgg gctccctcac ccggcggaag
      661 ccacggttta aggcgtttac aaacgggacc ttccgagtta acgcaagcat tgatgcggct
      721 acagttcagc cagtaaactt gcgcatgtag gaggagaaca tcatgcgcac cttcccccgt
      781 gtccgctccg tgctgctcgc tctcatgctg ctcagtgcgt cgaccgcgct gctggctcaa
      841 attcgcatct cggtcgcgtt cggtcccccg gccctccccg tctacgaaca gccaatctgt
      901 cccggcgacg gttacatctg gacgccaggc tattgggcct gggatccgga tgcagaggac
      961 tactactggg tgcccggcac atgggtactc gcgcccgaag tcggctacct ctggactcct
     1021 ccctggtggg gatgggaggg cggcaggttc ttcttccatg agggctggtg gggcccgcat
     1081 gtcggcttct acggcgggat caactacggc ttcggttatt tcggtgccgg cttcgtcggc
     1141 ggacgctggg acggcggaca ctttttttat aaccgcgaag tcacgaacat caatgtcgtc
     1201 aacatccaca acgtctacaa cgaacacatc aatgtcaccg tcaaccacat cagctacaac
     1261 gggggccccg gcggcctggg agcacggccc cggccggagg aagaaagggc ggagcacgag
     1321 cgccatatcg gcccggtcgg cgcgcagacc cagcactggg aggctgcccg cgggaatcgt
     1381 gaattgcgcg cttcggtgaa tcaaggcaga ccgcccatcg cagctacggg gcgccccggc
     1441 gaattttctg gacatgaggt tgtgaatgca cgggaagccg gcgcaccgta ccatccgccc
     1501 gccgaacgaa gcggtacggc tccgcgcggc ggaaatgcag gattcggttc cccggttcat
     1561 cccaaggagc ttccgccctt tgaacgccct gcggccccca acaccggcga taccaaccgt
     1621 gaccgaaaat accaacagca gcaggacaag ctgtttgcta aacaggaaca ggagcggcag
     1681 aacctgcagc agaagcagga gcaggaacac cagcgaatgg agcgccagaa cgctggccag
     1741 ccgcaacgtc aacagatgga acagcgtcac cagcagcaaa cccagcagat gcagcagaag
     1801 cacgtgcagc agcagcagca tctgcaggac cggcaacagc cgcaccggag gtagcgaaga
     1861 ccggcgggcg caactgctct tcactctggc ccgggaaaca ttcggaaatg ggtggaccac
     1921 ccgcccttgg aatccctctt gggtacctct ccgaagcacg agcgttaaga caattgattt
     1981 gctgaacacg cgttcgcgta cacgcccaag acgcgtcgta tgtgatactc aatcatccgc
     2041 tcgggagaag cctgcagttc tccagcacgg accgcctcgt actcggccgg caggtactga
     2101 ctgataagag ttggcgggat ggacccagcc gcgagattct cgaacagtcg cgcaacttct
     2161 ttttgcacct caggctggtt ccagtagtat cggcaccgat cgctatagcc aaacgtgaga
     2221 tcgcgccgca cctcttcctc atctccccgg tagtacgact gccagtaacg cggatcgcgg
     2281 cgcatcgccg cctccagagc ttcccgcact tgggatatct gccgctgccc cgtcgccttg
     2341 aacatttccc gctcaatgcc gctcaaagcc agcagcgcct cgcggtaggc aaacgtcagc
     2401 cagggcccga ccttcaggat cgcgaagtga tccctgacca ggtgagccag agccgaggga
     2461 gtctggtaat cggtcgagtg cgcctcatag acaattcccg gatgacgggc aagtgcggcc
     2521 gagagcgcct gcgctttcac cggatcgtag tcgaagacgg tgttcgatcc gaaatcaacc
     2581 cccggttgaa caacgagagc gatgactcgt tcccacgcgg acgattgccc ctgctgcacg
     2641 aaggcaagcc ggaaagtttc cattgtctgg tgcacatgtt cggcggtggt cacggcgggg
     2701 gcatgcgcgt cgactgattc tccaccgggc gcgggaactt cggtcccg
//
LOCUS       DASPUL010000349         3415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_61300, whole genome shotgun sequence.
ACCESSION   DASPUL010000349 DASPUL010000000
VERSION     DASPUL010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61300"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            450..1076
                     /locus_tag="VEJ00_08380"
     CDS             450..1076
                     /locus_tag="VEJ00_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01215.1"
                     /translation="MPDHHPLNEFSPKELRKLRSLKNPYGVQNFLDDMPYHLEDTAWS
                     PRKVLAHQTAHCLEGAIFAAAALRANGYPPLLLDFEADYDTDHVLAVFRERGCWGAVA
                     KSNYAGCRWREPIYRSLRELALSYFNIYFNMRRERTLRRYSLPVNLRRFDRQHWMTTD
                     EPVWFIVYHLFDIKHYPLLTKAQEKRLHRVDERLFQAECLGKAHKKPD"
     gene            1210..1626
                     /locus_tag="VEJ00_08385"
     CDS             1210..1626
                     /locus_tag="VEJ00_08385"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXX01216.1"
                     /translation="MVTTGAGEPIQERSTVELPNQSGTSQPRGVVDARRHPRFKLEVN
                     ICVYPRNTLVVRGYTVDISLSGISAILKDEVPLGEVVRLTFRLPLGEVEAHALVRQKN
                     AFRYGFQFVEASSAQDLIGQTCRDLAVKQIHSPGPS"
     gene            1771..2745
                     /locus_tag="VEJ00_08390"
     CDS             1771..2745
                     /locus_tag="VEJ00_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01217.1"
                     /translation="MKIWGLALILSLLALPALGQQDQPASAAPPADIDRPSEGGEAAR
                     AMATPKLDLTPDANGKLSQEQMQQLFRVVADKDLENDKRQRDYTYVERDVENRLDGKG
                     KTKSTETKTYDVLEIYGEPVQKLIEKDDKPLSEKEAAKEDEKVQKIIDKRKNESDEDR
                     KKREQREEKNREDDRKFVTEIADAYNFTLVGTEQINGRDAWIVDGEPRPGFEPRMKEA
                     KFLPKFHGRVWIDKDDQQLVKMDVEAIDTVSVGWFLARVHKGTRVMYEQTRVNDEVWL
                     PQHVTFKVDVRLALLKNFNIDGEQTYRDYKKFRTSAKIVGVGEVQDPK"
     gene            2887..>3415
                     /locus_tag="VEJ00_08395"
     CDS             2887..>3415
                     /locus_tag="VEJ00_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01218.1"
                     /translation="MSKSRREFLTLTSLGVLGVAAAQRVFAQAAANLPPGSPGAFGAG
                     PPVGPEVSVTTFAEAEKLVQFSLTDAERAQAAASWRRTLASVYERRVGQRKIALESTL
                     APASRWDPMLPGVKAGPQGDRFVRSRDTAAALPAKEEDIAFATVTQLSRWIEKRQLSS
                     ERLTRIYLDRLEKFNP"
BASE COUNT          734 a         1036 c         1010 g          635 t
ORIGIN      
        1 cagaggacgc ggagaactgc cgcgaaggtc gcgaagaaat tcctctgccg gggcaacctc
       61 ttggatgcat gcaccgggca tggtgcagtt tcgtataaca gtctttttct tgtgcggctt
      121 ccaaacctcg actcctgtga caaaaattgc ttctaattcg ctcattataa acaagttaga
      181 gtcgtgagtt aaatcgattt aggagctcgc tgcaaggccc cgaaatgcac cccgttccca
      241 agtacttgcc aacgggcgat cgttcatcag cggggtacgc gagcaccgcg tgggccattt
      301 cttaaactga gcggcatgcc atcctcttgg tccacgcgac ctcagtggga tcttcacggc
      361 aatctgcgat ctcaggatac ggaagtgccg gtccgtggct atccctccta cgctacactg
      421 ctggcacgaa gcaacgttca aacgaaacca tgcccgatca ccatcccctg aacgagttca
      481 gcccgaagga actgcgcaag ctgcgcagtc tgaagaatcc ctacggcgtg cagaattttc
      541 tcgacgacat gccctaccac ctggaggaca cggcgtggtc gccccgaaag gtgctggcgc
      601 accagacggc gcattgcctg gagggcgcga tcttcgcggc ggcagcgttg cgcgccaacg
      661 gatatccacc gctgctgctc gacttcgaag ccgactacga caccgaccac gtgctcgccg
      721 tttttcgcga gcgcggttgc tggggcgccg tggccaaatc gaactacgcc ggatgccgct
      781 ggcgcgagcc catctaccgc tcgctgcggg agctggcgct gagctatttc aacatttact
      841 tcaacatgcg gcgcgagcgc acgctgcgcc gttattccct tcccgtcaac ctgcgccgct
      901 tcgaccggca acactggatg accaccgacg agcccgtctg gttcatcgtc tatcacctgt
      961 ttgatatcaa gcactatccc ctgctgacca aggcccagga gaagcggctg caccgggtgg
     1021 acgagcgcct gtttcaggcc gaatgtctgg gaaaggccca caagaaaccc gattagcccc
     1081 cggtttccga cggcgaaggt ctgcgtcaat ccaatcgaag ctcgaaataa cgcctgccgt
     1141 actaaagtct ggccttactt tggccgataa tggcgggtac ggtctgcatt tgcccgccgg
     1201 cacgttcgta tggtaacgac gggcgcgggt gagcccatcc aggagcggag tacagtggag
     1261 cttcccaatc agtccggaac gagccaaccg cgaggcgtcg tggacgcgcg ccgccatcca
     1321 cgcttcaagc tggaagtgaa catctgcgtg tatccgcgga ataccctggt ggttcgcggc
     1381 tacacggtcg acatcagtct ctccggcatc tccgccattt tgaaagacga agtgccgctg
     1441 ggcgaagtgg ttcgtctgac gtttaggctg cccctgggag aagtggaggc gcatgcgctc
     1501 gtccgccaga aaaatgcctt ccgctacggc tttcagttcg tggaagccag ctcggcgcag
     1561 gacctgatcg gccagacctg ccgcgatctg gccgtgaagc aaatccacag tccgggccca
     1621 tcgtaatctt tccccagcct ggcgtccgaa tcggcgatcg actcgcgacc ggctggccct
     1681 gtcctggccg caacctttcg aatccaaccg ggttgtagcg tttgaattcc tgtgggaacc
     1741 tttgattccg aaaaagcgta aggtcaggat atgaagatct ggggtctcgc gctcatccta
     1801 tccctgcttg cactgccggc gctgggtcag caggaccagc ctgcctcggc agcgccgccg
     1861 gccgacatcg atcgtccatc cgagggaggt gaggcggcta gggcgatggc tacccccaaa
     1921 cttgacctga cgcccgacgc caacgggaag ctttcgcagg agcagatgca gcaactgttc
     1981 cgcgtcgtcg ccgacaagga tctggagaac gacaagcgcc agcgcgacta tacctacgtg
     2041 gagcgcgacg tcgagaaccg gctcgacggc aaaggcaaaa ccaagtcgac ggaaaccaaa
     2101 acctacgatg ttctggaaat ctacggtgag ccggtgcaga aactgatcga aaaagacgac
     2161 aagccgcttt cggaaaagga agcggccaag gaagacgaga aagttcagaa gatcatcgac
     2221 aagcgcaaga acgagtcaga cgaagaccgc aagaagcgcg agcagcgcga agaaaagaat
     2281 cgcgaagacg accgcaaatt cgttaccgag atcgcagacg cctacaactt cacgctggta
     2341 ggcaccgagc aaatcaacgg ccgcgacgcc tggatcgttg acggcgagcc gcgtcccggc
     2401 ttcgagccca ggatgaagga agccaaattt cttccgaagt tccacgggcg agtctggatc
     2461 gacaaggatg accagcagct ggtcaagatg gatgtggaag ccatcgacac cgtgtcggtg
     2521 ggctggttcc tggcgcgcgt gcacaagggc acgcgcgtga tgtacgagca aacgcgggtg
     2581 aacgacgaag tctggctgcc gcagcatgta accttcaagg ttgacgtgcg cctggccctg
     2641 ctcaagaatt tcaacattga cggcgaacag acctaccgcg actacaagaa gttccgcacc
     2701 tccgcgaaga tcgtgggggt aggcgaagtg caggacccaa agtagtcttt cataccaagc
     2761 cgatgttccc gtcggctgta tgctcgtaaa caatcccggg ctgtctatgt ttcacccttc
     2821 gcgcctcgaa tcccgacgcc gactctttcc tgtaagattc tggagccgca ggcaccaggg
     2881 aggatcatgt cgaaatctcg ccgggaattc ttgacgctta catcgctcgg ggttcttgga
     2941 gttgccgctg ctcagcgcgt cttcgcgcaa gccgctgcga acctgccgcc gggctcaccc
     3001 ggcgcgttcg gcgccggacc gccggtgggg ccggaggtct cggtgaccac gttcgcggaa
     3061 gccgagaagc tggtgcagtt ttccctgacc gatgccgagc gcgcccaagc cgccgcaagc
     3121 tggcgccgaa cccttgcgag tgtttacgag cggcgcgtgg gtcagcggaa gattgctctg
     3181 gaatcgactt tggcgccggc ttcgcgctgg gaccccatgc ttcccggcgt caaggccgga
     3241 ccgcagggcg atcgcttcgt gcgcagccgg gacacggctg cggccttgcc tgccaaagaa
     3301 gaagacattg cctttgccac ggtgacgcaa ctctcgcgct ggatcgagaa gcggcagcta
     3361 agttcggagc ggctgactcg tatttatctg gaccggctgg agaaattcaa tccca
//
LOCUS       DASPUL010000350         4396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_207368, whole genome shotgun sequence.
ACCESSION   DASPUL010000350 DASPUL010000000
VERSION     DASPUL010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207368"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VEJ00_08400"
     CDS             <1..281
                     /locus_tag="VEJ00_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXX01219.1"
                     /translation="GGCDSVGPADYNYDLSQRRANSVIQYLASKYSVPAHKIYVIGLG
                     KDKPVETNKTAAGRADNRRVDVRLMTNTTGGNAPASTPATTGSNPSSM"
     gene            671..1348
                     /locus_tag="VEJ00_08405"
     CDS             671..1348
                     /locus_tag="VEJ00_08405"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX01220.1"
                     /translation="MRRGFLILVLLLSAGVAWAQSSSAPGKTPDGSSSPSGQSPEASA
                     PTSSAPSQTSQPSAKFPPANPEPPRSDSVNAATLDNEPGESSSKDTQIDLSPPPGDDK
                     SHPQSSDTLTDVEAAPGGASVGEFHPWDPHKAAKDIEVGDFYFKRKNYRAAEDRYREA
                     LLYKDNDANATFRLAECLEKMDRPAEARQQYERYLKILPHGPEAERAAKAIERLKGPS
                     AEAKPAK"
     gene            complement(1359..2030)
                     /locus_tag="VEJ00_08410"
     CDS             complement(1359..2030)
                     /locus_tag="VEJ00_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXX01221.1"
                     /translation="MPRQPDPALEQRILDAASRLWARGGEKTLTMRAVAKAAGTTTPT
                     VYERYRDREDILRALRLKTRRDLFAALNRTRTLRQAVHCHLEFALEHSHAYEVLFDGV
                     AQPPSLHESWPSFNLIRERVAQRIGGTPRQHTRLMLALWSLMHGTAMLIIRGRFEGPL
                     RTQSVHSCLDVFDAIVDSATRKKGIAHSGPKWPPNLILGEGKQSRVGDGEGNNRRKSR
                     TRTKR"
     gene            2235..2492
                     /locus_tag="VEJ00_08415"
     CDS             2235..2492
                     /locus_tag="VEJ00_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01222.1"
                     /translation="MVSAVKKHVDVVRLTADLAAAKVELLSAQCATDRIRLQYSPQDV
                     VAFGERQTLERMIASAEAVCRFFTQIGAQIKHAEESGHGKK"
     gene            complement(2555..3244)
                     /locus_tag="VEJ00_08420"
     CDS             complement(2555..3244)
                     /locus_tag="VEJ00_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HXX01223.1"
                     /translation="MKTARYSRRACDRKGISLGTLYAWFSAVLILALLALLVGCSSES
                     GKPAESAKPEVKGPDLITARSAFQKVYVAARGWAQDAKPYRLESIVTTDGNGHDGKWA
                     EWRGSFASDMHRSAKSYVWSGSAASGAPERGVNPGTEDSYSPTNASTKVFDMAFLKID
                     SDQALATAQKHGGEKILESAPDTPVFYVCDWNHNTNELVWHVIYGASRETAKLTVAVN
                     ASTGEFIRVEK"
     gene            3328..3873
                     /locus_tag="VEJ00_08425"
     CDS             3328..3873
                     /locus_tag="VEJ00_08425"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX01224.1"
                     /translation="MDPQLETLQRALASAVSGLTSEQLSWHPPGKWCATEVLEHLYLT
                     YTGTTKGFQRLADAGKPLATRATWRQVGRTFVVTGLGYFPSGREAPSVARPRGMAPEK
                     VVTEIAARISEMDDSIRACEEKFGRQQKVLDHPILGPLNSRQWKKFHLVHGLHHVKQI
                     WRLREGAVRSRSAGLMNSGGI"
     gene            complement(3966..>4396)
                     /locus_tag="VEJ00_08430"
     CDS             complement(3966..>4396)
                     /locus_tag="VEJ00_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01225.1"
                     /translation="AQAAPAESAAAPASADNKAQTDNKASAPAQTASTAKTPEEDQAA
                     KQAAWKQWGEKIAAQKEQIDLLTRELTVLQREYQIRAAAMYSDVGNRLRNSGDWDKQD
                     AQYKQQIADKQKQVDDAQQKLTDMQEEARKAGVPSSIAEP"
BASE COUNT          818 a         1291 c         1436 g          851 t
ORIGIN      
        1 aaggcggatg cgactcggtg ggaccggccg actacaacta cgatctcagc cagcgccgcg
       61 ccaattcggt gattcagtat ctggcgtcga agtacagtgt gccggcgcac aagatctacg
      121 tgatcggcct gggcaaggac aaaccggtcg agaccaacaa gacggcagcg ggccgcgccg
      181 acaaccgccg cgtggatgtg cgtctgatga ccaacaccac gggcggcaac gcaccggcgt
      241 caactccagc gaccacgggc agcaatccgt cgtcgatgta agagctgtga gccccgagtc
      301 gtgagccgcg aggcgaccgc atcatagctg gtcgttcaaa gctcgaagcc cacggctcga
      361 agctacaagt ttaccctcct ccccggagga cttcgccaac caggccgctc aacacctccc
      421 cctgagcggc ctgttttttt gtctttgcaa aatcggctga gtggaacgat ccctacaggg
      481 cgaccacggg ggccaaggcc ccgtgttctg gggccgtgac gtggccctga agggccactc
      541 ttccacggta gcgggtccgg cagcagcgtc acataagacg gccccggcag gcttgcctgc
      601 cgcaacggga gttacggtag aggcggcgta gctccggcga cgacgctttt ggcggagtta
      661 aaatggctgt atgcgtcgcg ggttcttaat cctggtcctg ctgctgagtg ctggggtggc
      721 gtgggcgcag agttcatctg ctcccggcaa aactcctgac ggttcgtctt ctccgagcgg
      781 gcagtcgccg gaagcgtctg cgccaacttc ttccgcgccg agccagacga gtcagccttc
      841 tgcgaagttt ccccctgcga atccggagcc gccgcgatcg gacagcgtca atgcagctac
      901 tctggataac gaaccggggg aaagttcgag caaagacacg caaatcgatt tgagtccccc
      961 gccgggcgac gacaagtccc atcctcagag ttccgacacg ctgacggacg tcgaggcggc
     1021 gccgggcggc gcgagtgtgg gcgagttcca tccctgggat ccgcacaagg ctgccaagga
     1081 tatcgaggtc ggcgactttt acttcaagcg caagaactac cgtgctgccg aagaccgtta
     1141 tcgggaagcg ctgctctata aagacaacga cgcgaacgct actttccgcc tggctgagtg
     1201 tctggagaag atggaccggc ctgcggaagc acgccagcag tacgaaaggt acctgaaaat
     1261 cttgccgcat ggtcccgagg cggagagggc cgccaaagcg attgagcggc tgaaggggcc
     1321 gtcggccgag gccaagccgg cgaagtaggc cgaaagcctc agcgcttcgt ccgggttcgc
     1381 gatttcctcc tgttattacc ttcgccgtct cccacgcggc tctgcttacc ctcgccgaga
     1441 atcaggttcg gaggccactt cggcccggag tgggcgatgc ctttctttcg cgtggcggag
     1501 tcgacaatcg catcgaacac gtcgagacaa gagtggaccg actgcgtgcg caacgggccc
     1561 tcaaagcggc cgcgaatgat cagcatggcg gtgccgtgca tcagcgacca aagcgccagc
     1621 atgaggcgcg tgtgctggcg cggagttccg ccgatgcgct gggccacgcg ctcgcgaatg
     1681 agattgaacg agggccagct ttcatgcagg gacggcggct gggccacgcc gtcgaacagc
     1741 acttcgtagg cgtggctgtg ttcgagggca aactccaggt ggcagtgcac ggcctggcga
     1801 agggtgcgcg tgcggttgag agccgcgaac aggtcgcggc gggtcttgag gcgcagcgcg
     1861 cgcaggatgt cttcgcggtc gcggtaacgc tcgtagacgg tgggggtggt ggtgccggcg
     1921 gctttggcca cggcgcgcat ggtgagggtc ttttctccgc cgcgggccca caggcgggag
     1981 gcggcgtcaa gaatgcgttg ttcaagagca ggatcgggtt gtcgcggcac tttctctcca
     2041 gggtcagaaa acgtgcgaat taaatcgaat taattacttg cactgcgttt cattaacgct
     2101 gtatacttat ttcaacgtag caacaactga cggcctcgcg ggtggcttcc gccggaacct
     2161 gaagaacggc agttctcccc ataaggttcc cggatgccac ctgcgttcat ttcataacag
     2221 tcgaggagca gtagatggta tctgcagtga agaagcatgt tgacgtcgta cgcctgacgg
     2281 cggacctggc cgccgccaag gtggaattgc tgagcgcgca atgcgccact gaccggattc
     2341 gtctccaata ttccccccag gacgtcgtgg ccttcggcga acggcagact ctggagcgga
     2401 tgatcgcttc ggccgaggcc gtctgccgct tttttaccca gatcggggcc cagatcaagc
     2461 acgcagaaga atcaggccat ggcaagaagt gacgcacgcc acgggccctt ttccttccgg
     2521 tatatatcga cttaatggcg ctgcaggggc aagactattt ctccacgcgg atgaactctc
     2581 cggtggaggc gttgactgcg accgtcagct tggcggtctc gcggctggcg ccgtagatca
     2641 cgtgccagac cagctcgttg gtgttgtggt tccagtcgca gacgtagaag acgggcgtgt
     2701 ccggagcgct ctcgaggatc ttttcgccgc cgtgcttctg tgccgtggcc agggcctggt
     2761 cggaatcaat tttcaagaag gccatatcaa agactttggt ggaggcattg gtcgggctat
     2821 agctgtcctc cgtgccggga ttgacaccgc gttcgggcgc gccgctggcc gcgcttcccg
     2881 accacacgta agacttggcg gaacgatgca tgtcggaggc gaagctgccc cgccactccg
     2941 cccacttgcc atcgtgaccg ttgccgtcag tggtaacgat ggattccaga cgatacggct
     3001 tcgcatcctg cgcccagccg cgcgccgcca cgtagacttt ctggaaagcg gagcgcgcgg
     3061 tgatcaggtc cggcccttta acctcgggtt tcgcgctctc tgccggcttg ccggactccg
     3121 aagagcagcc caccaatagc gcaagaagag cgagtatgag aacggcggaa aaccacgcgt
     3181 acaaagttcc cagcgagatt cctttccggt cgcaagcgcg ccggctataa cgggccgttt
     3241 tcattggcat ccttcctggt ataaggttgt gggtttattg acgcgcaaca ttctacccga
     3301 agcgttgcaa aaggctaagt aatactcgtg gaccctcaac tggaaaccct acagcgagcg
     3361 cttgcctcgg cggtcagcgg actgacgtcg gaacaattga gctggcatcc acccggaaag
     3421 tggtgtgcca ctgaggtgct ggagcatttg tacctgacct acaccggaac cacgaaagga
     3481 ttccagcgcc tggccgacgc gggaaagccg ctggcgacgc gcgcaacgtg gaggcaggtc
     3541 gggcggactt ttgtcgtaac gggcctgggc tactttccgt cggggaggga agcgccctcg
     3601 gtggcgcgtc cgcggggaat ggcacccgag aaagtcgtga ccgagatcgc ggccagaatc
     3661 tccgagatgg acgactcgat tcgggcgtgc gaggaaaaat tcggcaggca acagaaagtg
     3721 ctggatcatc cgatcctggg cccgctcaat agccggcagt ggaagaagtt tcacctggtg
     3781 cacgggctgc atcacgtaaa gcagatctgg cggctgcggg agggcgcggt tcggagccgt
     3841 tcagcggggt tgatgaattc gggagggata tagccgcccg cgatgctttg gctcccagac
     3901 tcgctgcgct cgtgggacag ccgaggtggc tgtccccaca tggcgcgttg agtggcgcag
     3961 ggttcttacg gttcagcgat ggacgatggg acgcccgcct tgcgggcctc ttcctgcata
     4021 tcggtgagtt tctgctgtgc gtcgtcgacc tgtttctgct tgtcggcgat ctgctgcttg
     4081 tactgtgcgt cctgcttgtc ccagtcgccg gagttgcgca ggcggttgcc gacgtcggaa
     4141 tacatggcgg cggcgcggat ctggtactcg cgctgcagga cggtgagctc gcgggtcagc
     4201 aggtcgattt gttccttttg cgcagcgatc ttttcgcccc actgcttcca agcggcctgc
     4261 ttggcagcct gatcttcttc gggcgttttc gcggtgctcg cggtctgcgc aggcgccgag
     4321 gccttgttgt cggtttgggc cttattgtcg gcggaagccg gcgccgcggc tgattcggcg
     4381 ggcgcggctt gcgcag
//
LOCUS       DASPUL010000351        12640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_613199, whole genome shotgun sequence.
ACCESSION   DASPUL010000351 DASPUL010000000
VERSION     DASPUL010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 12640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12640)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12640
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613199"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VEJ00_08435"
     CDS             <1..183
                     /locus_tag="VEJ00_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01226.1"
                     /translation="LVLVEIQPIRADDARGLLPAVLQRMQAQVSELFRFGVGENRHDT
                     ALVMKFVGVMHLAPDS"
     gene            complement(219..2528)
                     /gene="purL"
                     /locus_tag="VEJ00_08440"
     CDS             complement(219..2528)
                     /gene="purL"
                     /locus_tag="VEJ00_08440"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HXX01227.1"
                     /translation="MPNPVTCSFSAETLRQHGLTPDEFEKIKHLLGDREPTLTELGIF
                     SVMWSEHCSYKSSRVHLKRLPTRSKRVMQGPGENAGIIDIGDGWACAFKIESHNHPSF
                     IEPFQGATTGVGGILRDIFTMGARPVAVMDSLRFGPITPSGSIAQKELHKNHSILEGV
                     VSGVASYGNCFGVPNLGGETKFELCYSGNPLVNAFALGLVRKDEIFYARAAGEGNPVI
                     YVGAKTGRDGIHGATMASEEFSEASEAKRPNVQVGDPFLEKLLLEACLEAMQTGAIVG
                     IQDMGAAGLTCSTCEMGARGRVGIEIELDCVPQRETGMTPYEIMLSESQERMLLVAQK
                     GREHEVFRVFEKWGLDAVAVGKVTTDNRLRVLQHGKVVAEIPNQALTDDAPVYKRPLE
                     RWEPPVPREMPQHVRLGGTGDLTRDLRRLLASPNICGKRWVWQQYDHMVQTNTVEAPG
                     AGDAGVIRIKGSQRGLAMALDGNGRWCYLDPRLGAMHAVAEAARKVACSGATPVAATN
                     CLNFGNPEKPHIMWQFSQTIDGITRACEELEVPITGGNVSFYNETLGEGIYPTPVLGV
                     VGILEDVQKAAAMHFRAAGCTIVLLHAGEEADITDVESEFGSSEYAKEILGSVWGYPP
                     ELDLEKEGALQKAVVELIQQSLVDSVHDCSDGGIAVALAEKTFPRMIGANMNIAANNL
                     PSEFVLFGEDASRVVISCDPQSVMRIRQIAEKHGITADAIGETGGDRLEISLDGKTAV
                     TVTLAELNSEYESALESALRADPELVAAD"
     gene            2625..4580
                     /gene="selB"
                     /locus_tag="VEJ00_08445"
     CDS             2625..4580
                     /gene="selB"
                     /locus_tag="VEJ00_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HXX01228.1"
                     /translation="MKSIIIGTAGHIDHGKTALVKALTGIDADRLQEEKKRGITIDLG
                     FAHMDLSGANGEALRLGFVDVPGHERFVRNMLAGVGGIDLVLLVIAADESIKPQTREH
                     FDILQLLGVRRGITVLTKSDAVDAETREVVRLEVEEFLRGTFLEAPNSPIVPVSSLAG
                     TGIESLKSALALAAAQVEVRDSNAVARLPIDRVFTMKGFGTVVTGTLISGTIRRDDEL
                     EVFPTGRTVRVRGIQVHGQGAEAALAGQRTALNLAGASTEQLARGMTLTPPTTFNATD
                     RVDVKLRLLGSAPRALKTRARVHFHSNTMETIAEVVLHDAKQVTPGSAALARLKLPAP
                     ALLLPGDRFIIRQFSPVVTIGGGVVLDAAAPMWQNVSKGRARTGRASSLRDLAHLMQT
                     LESGNNEAILGIRVTRRGRAGLSLAEAVAETGWPREAVLKLSAVLVNQGLLARFQDRM
                     FAASDFALTRQDLINAVSSFHVKDPLRQGMTKSELQDEMTRGFGVAAELFDAALASLV
                     GQRNIEAAGDLVRLPGHGAVMKDEEAESKRKIEEAFAAAALKVPALHEVIAGLKIDKA
                     RAQKIVTLLLREKTLVKISDELVFHHGALDELRRQMAAYKAKSPKIDVANFKQLTGVS
                     RKYAIPLLEYLDRERVTRRVGDLREIV"
     gene            4614..5093
                     /locus_tag="VEJ00_08450"
     CDS             4614..5093
                     /locus_tag="VEJ00_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX01229.1"
                     /translation="MLRIRKATAHDAALLWTMIRELAAFEHELEFVTIRQEDLARDGF
                     GENPRFRAFIAEWDGQAAGYALFFDYYTTWTGRGLFLEDLFVREQYRGRRIGQTLLAA
                     VARIAVDERCYGIHWEVLDWNEKAIELYKALGAEFRDQWRPVLLSGEALLKLAEKSS"
     gene            5090..6445
                     /gene="trmFO"
                     /locus_tag="VEJ00_08455"
     CDS             5090..6445
                     /gene="trmFO"
                     /locus_tag="VEJ00_08455"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HXX01230.1"
                     /translation="MTARVRIIGAGLAGSEAAWQCARRGLEVELFEMRPVRSTPAHQT
                     AGFAELVCSNSLKSDSENTAPWLLKEEMRRAGSLLLRVARECAVPAGHALAVDRVAFS
                     AQVTERLLQEPQISVRREEVTAIDETSGVTIVATGPLTSEALTSEIARLTSALPGAAL
                     ANPHLYFYDSISPIVEADSIDMSRVYLAARYDKGSADYINCPMTAEEYNRFYHALLTA
                     QSVEERDWEKLNYFEGCLPIEEIARRGRDTLRFGPMKPVGLKDPRTGKMPYAVVQLRQ
                     ENLRAESYNLVGFQNHLNFGEQARVLRLIPGLENARFLRYGQIHRNTYINAPTLLQET
                     LQMKAHPRVFFAGQICGVEGYVESIATGLLAAIHAAALVSGEVLAPAPRSSAFGSLTH
                     YVTHASPENFQPANITFDLLPLLDKTVRDRKERHCLQCQRALQDFDRWVSGVGVTTLK
                     G"
     gene            6601..7884
                     /locus_tag="VEJ00_08460"
     CDS             6601..7884
                     /locus_tag="VEJ00_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX01231.1"
                     /translation="MSRGVQPAMTVRDLISDVLRTLWAHKLRAFLTMFGIAWGIVSIV
                     LMVAAGEGLRKGQEEQARTLGKDVMIVFHGRTSLQAGGTHAGRVVHWEDPDVPVVQAE
                     SPDCEYAIPELEQNTVRTHSNFNNAAFTVTGSYPQFAYIRSLDVGAGRFYDWDDMREA
                     RRVAFLGSDAAKQLFPGRNPVGDTIYLNDFPYTVIGVMAKKKQDSSYDGWDVNKVFLP
                     FAAMRRDFPDKPPGTNTTFDQLLVTPKSVDQHDACKHEVRVALARMHRYDPNDKEACP
                     IWDTVQEAQAFETMTDGMKFFLGAVGIVTLLLGGIGVMNVMLVAVRERTREIGVRKAI
                     GAPASTILRQFFIEALIIALLSGGIGLGVAFGFCALVNLLPMPDFFAGLLPSWSSGLI
                     AAGLLGTIAVGAALYPARRAAAVDPIEALRYEAGG"
     gene            7922..9178
                     /locus_tag="VEJ00_08465"
     CDS             7922..9178
                     /locus_tag="VEJ00_08465"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX01232.1"
                     /translation="MLIEIILEAWIALKRNVTRSLLTMLGIVWGIATVTLLIAYGSSF
                     RSILVHGFDAFGKSVVICWPQQTSEQPGGQRAGKKVVLEQADLDLVKETASLVKHACL
                     ETVERPGIAYGDRIAGTSPVRGVCPEYGEMRNEVPSEGRWINAEDELERRRVIFLGGR
                     LKEQLFSGRPAVGETVQVAGVRFTVIGVMARKIQLSNYFSSDDDSSWIPYSAAGDLWN
                     TRYAAVMVFEPIAPQFEKKAMAQVLAALATRQNFSPTDPKALQMFGRDEFRPIIDALT
                     IGLQVLLTFIGTLTLGIGGVGVMNIMLVSVDERIREIGLRRALGARKRHIKLQFLAET
                     LLLMLLGGAIGVLLSYVVAWSVGTLPMMGPLFEDDSGQGDIHLRISMLTVMLSTAVLL
                     VVGIISGLVPAMRASRLDPVEALRYE"
     gene            9327..10421
                     /locus_tag="VEJ00_08470"
     CDS             9327..10421
                     /locus_tag="VEJ00_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01233.1"
                     /translation="MPISAVDTITLAFQHTKRQLLQPFRFWQWTRLALVGLLAGEMGS
                     GGGSFRFPSSFNVPQQSGSSRHFLGSPFGSVDPALLVVLIAVIVTAALVFGVIMMYIS
                     SVMRFILFDSVLAKECHIRQGWNRRQGPGWRYFLWQLVYMLATFAGLVVLVGIPAGLA
                     YAMGWFKQPSQHVLGLVLGGVVVFFLFAAFMVTAAVVYVLTKDFVVPQMALEGIGVME
                     GWRRLWPMLQGEKSGYAGYIGMKIVLAIGAGIVIGIASLILVLMLAIPTAAVVLGAIF
                     AGKSAGLTWNVFTITIAVVVGCVLFALLMYVLALISVPAIVFFPAYSIYFFAPRYRAL
                     SLALYPPPPPLAAPPEPPEPPPPLAPAPAT"
     gene            complement(10452..11297)
                     /locus_tag="VEJ00_08475"
     CDS             complement(10452..11297)
                     /locus_tag="VEJ00_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01234.1"
                     /translation="MARLISIPHFAIFVSVVLPALSQSAFTQDNYEIQVYSYDTVAPR
                     STMVEIHSNFTVDGSKTVQDGILPTNHAEHETLEITQGINNWAEVGFYLFSSIQPNDG
                     WQWVGDHIRPRVRVPDSWHWPVGVSVSNELGYQRHAFSADTWTWEIRPIVDKKIGPWY
                     LDFNPTLDRSFHGPGVNQGVVFSPNAKISYDFTKKIAGGLEYYASYGPITGFDPLQDQ
                     QQQFFPTIDVDFGPEWEFNFGVGVGTTRSTDHLIVKCIIGRRFSWPHGKGTKLKSPDE
                     QQKNR"
     gene            11474..11920
                     /locus_tag="VEJ00_08480"
     CDS             11474..11920
                     /locus_tag="VEJ00_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXX01235.1"
                     /translation="MAFSTSGAGGGARAEINVTPLIDVLLTLIVMFMLVVSMDKQYQE
                     DAQIPQPGKQPTAGEEQSRTVVIEVVWTSQDRPPSVKINKQDISWEDLGGQLQKIYLT
                     RAEKVAFVRGDADVDFQYVADVIDRAHHAGVQRIGLLTMDRTVEGE"
     gene            complement(11939..>12640)
                     /locus_tag="VEJ00_08485"
     CDS             complement(11939..>12640)
                     /locus_tag="VEJ00_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXX01236.1"
                     /translation="NGRRFRFIDTAGIRRKGKTHLMAEKLSVVMARKHLESADIALLV
                     IDATEGVSQLDAAIAGYAHESGRSVIIVVNKWDLVISGKAKSARPTKAERMRESKRPA
                     DRTAYEQRLLYELKFLNYAPVVFVSAKSGKGAENIFPLLEQVATERRKRITTSEMNRF
                     LERVDFDRASVPMRQRVKILYMTQASVAPPTFVLFTNRAVKLHFSYQRFLENQIRQAF
                     GFLGTPIWIKARARE"
BASE COUNT         2444 a         3681 c         3830 g         2685 t
ORIGIN      
        1 cttgtgctgg tggagattca gcccatccga gctgacgatg cccgcggact cctgcccgcg
       61 gtgctgcaac gcatgcaggc tcaggtaagc gagcttttcc gcttcggggt gggcgaaaat
      121 cgccacgaca ccgcactcgt catgaaattt gtcggcgtga tgcatcttgc tcctgactct
      181 tagctgtcga ctcctttggt gaaaaccgac gctcaaattc aatctgcagc caccagttcc
      241 ggatcggctc gtaacgccga ctccagagcg ctttcgtatt cgctatttaa ctccgcgagg
      301 gtcacagtca cagccgtttt gccgtcgagc gaaatctcca ggcgatcccc gccggtttcc
      361 ccgatggcat cggcggtaat cccatgcttc tccgcgatct gtcggattcg catcacgctc
      421 tgcggatcgc aggagatcac aacccggctc gcatcttcgc cgaacagcac aaactcggac
      481 ggaaggttgt tagcggcgat gttcatgtta gcgccaatca ttctggggaa tgtcttttcc
      541 gccagtgcca cggctatgcc accgtcagag caatcgtgaa ccgaatccac cagactctgc
      601 tgaatcaact caacgacggc cttctgcagc gccccttcct tctccaggtc gagctccgga
      661 ggatatcccc agacagaccc tagaatctcc ttcgcatatt cggaagaccc gaattcactc
      721 tccacgtcgg tgatatccgc ctcttctccg gcgtgcaata gcacgatcgt gcagcccgcc
      781 gcgcggaaat gcatcgcggc cgccttttga acgtcttcga gaatgccgac tacaccgagc
      841 acaggcgtcg gatagattcc ctcgccgagg gtctcgttgt agaaactgac gttgccgccg
      901 gtgatgggga cttccaactc ttcgcacgct ctggtgatgc cgtcgatcgt ttgcgagaac
      961 tgccacatga tgtgcggttt ctcaggattg ccaaagttca ggcaattcgt ggccgccacg
     1021 ggagtggctc cagagcatgc tactttgcgc gccgcttcgg caacggcgtg catcgcaccg
     1081 agccggggat cgagatagca ccagcgtccg ttgccgtcaa gcgccatggc caagccgcgt
     1141 tgcgagcctt taatgcggat cacgccggcg tcgccggcgc ccggcgcctc gacagtattc
     1201 gtttgcacca tatggtcgta ctgctgccat acccagcgct tgccgcaaat gttggggctc
     1261 gcaagcaacc gtctcagatc cctcgtcaga tcgccagtcc cgccaagtct gacgtgctgc
     1321 ggcatttcac ggggaaccgg aggctcccaa cgctccagcg ggcgcttgta tacaggagca
     1381 tcatcggtta gcgcctgatt cggaatctcg gcgacgactt taccgtgttg gagcacgcga
     1441 agtctgttgt ccgttgtgac cttgcccact gcaaccgcgt ccaggcccca cttctcaaat
     1501 acgcgaaaga cttcatgttc gcgacctttt tgcgcgacca aaagcatgcg ctcctgcgat
     1561 tcgctgagca tgatctcgta gggagtcatc ccggtctcgc gttgcggcac acagtcgagc
     1621 tcaatctcga tgcccacgcg accgcgtgca cccatttcgc atgtggagca agtcagccca
     1681 gccgcgccca tgtcctgaat gccgacgatc gctccggttt gcatggcttc cagacaagcc
     1741 tcaagcagga gcttctcaag aaacggatcg cctacctgta cgttgggacg ctttgcttcc
     1801 gaggcttcac tgaactcctc gctggccatg gtggcgccat ggatcccgtc gcgcccggtc
     1861 ttcgccccca cgtaaatcac ggggtttcct tcgcccgcgg cgcgcgcata gaagatttca
     1921 tcctttcgca ccaggcccag agcgaaggcg ttcactagcg gatttccgga atagcatagc
     1981 tcgaactttg tctcgccgcc gaggttcggc acgccaaagc aattgccata tgaggcgacg
     2041 ccactaacca cgccttcaag aatggagtga ttcttgtgca attctttttg agcgatgctc
     2101 cccgacggag taatcgggcc gaagcgcagc gaatccatca ccgccaccgg ccttgccccc
     2161 atcgtgaaaa tgtcacgcag aatgccgccc actccagtag ttgccccctg aaaaggctca
     2221 atgaacgacg ggtgattgtg cgactcgatc ttgaacgcgc acgcccagcc gtcgccgatg
     2281 tcgatgatcc ccgcattttc ccccggtccc tgcatcaccc gtttgctgcg cgtcggcaga
     2341 cgcttcagat gcacgcgcga ggatttgtag gaacagtgct cgctccacat cacgctgaag
     2401 atgcccagtt ccgtcaacgt gggctcacgg tcccccagca gatgtttgat cttctcaaat
     2461 tcgtcgggcg tgagcccatg ctgccggagg gtctccgcag aaaaggaaca ggtcacaggg
     2521 ttgggcatgg aactgcttct attgtcgcat ttccgggttg aggtgaatag ggcagaattc
     2581 ccgcccggct tgcgagagct ccgtcctgat acgattctct aggaatgaaa tcgatcatca
     2641 tcggcacagc cgggcacatc gatcatggga agacggcgct ggtgaaggcg ctgaccggca
     2701 tcgatgccga ccgcctgcag gaagaaaaga agcggggcat cacgattgac ctgggctttg
     2761 cgcacatgga tttgagcggc gcgaacggcg aggcgctgcg gctaggtttt gtggacgttc
     2821 ccggccacga gcgcttcgtg cgcaacatgc tggctggtgt gggcggcatc gatctcgtgc
     2881 tgctggttat cgcagccgat gaatccatca agccgcagac acgcgagcac ttcgacatcc
     2941 tgcaattgct gggcgtcagg cggggtatca cggttctcac aaaatcggac gccgtcgacg
     3001 ccgaaacccg cgaggtagtg cgcctcgaag tcgaagaatt cttgcgcggg acattccttg
     3061 aggcgccaaa ctcgccgatc gtaccagtca gctcgctggc tggcacaggg attgagagcc
     3121 tgaagagcgc tctggccttg gctgcggccc aggttgaagt gcgcgattct aacgctgtcg
     3181 cgcgcttgcc gatcgaccgc gtgttcacga tgaaaggctt cgggaccgtt gtgaccggga
     3241 ctcttatctc gggaacgatc cgccgggacg acgagctcga ggttttcccc acggggagaa
     3301 ccgtccgagt gcgcggaatt caggtgcacg gacagggggc tgaggctgct ctcgccggac
     3361 agcgtacagc cttgaatttg gccggcgcga gcaccgaaca attggcgcgt ggaatgacgt
     3421 tgactccgcc cacgacgttc aatgcgactg accgcgtcga tgtaaagctg cggctcctgg
     3481 gctcagcgcc gagggcgctg aagactcgtg cgcgcgtgca cttccactcc aacacgatgg
     3541 agacgatcgc agaagttgtc ctgcacgacg cgaagcaggt tactcccgga tcggccgcac
     3601 ttgcgcgctt gaagctcccg gcgccagctc tcctgttgcc aggcgatcga ttcatcatcc
     3661 ggcaattctc gccggtggtg accatcggcg gcggcgtcgt gctggacgcg gcggcaccta
     3721 tgtggcaaaa cgtgagcaaa gggcgggctc gcactgggag agcgtcgtca ttgcgagacc
     3781 tcgcacattt gatgcagacg ctggaatcag ggaacaacga ggccattctt ggcatccggg
     3841 tgacacggcg cgggagagca ggtctctcgc tggcggaagc cgttgccgaa accggatggc
     3901 ctcgcgaggc agttctgaaa ctttccgcag tactcgtaaa ccagggactg ctcgcgcgct
     3961 ttcaagaccg aatgttcgcc gcgtccgact tcgcgctgac ccggcaggac ctgatcaacg
     4021 cggtttcgag tttccatgta aaggatccgc tccgccaggg tatgacgaag agcgagctgc
     4081 aagatgagat gacccgtggc ttcggtgtcg ctgccgaatt gtttgacgct gcgctggcct
     4141 ctctggttgg gcagaggaac atcgaagctg ccggtgacct ggtgcgactg cctggtcacg
     4201 gcgcggtcat gaaggatgaa gaagccgaat cgaaaaggaa gattgaagag gcttttgccg
     4261 ctgcggcatt gaaagttccc gcattgcatg aggtgatcgc cgggctcaag atcgataaag
     4321 cgcgggcaca gaaaattgtc actctcctgc tgcgcgaaaa aacgctggtt aagatctccg
     4381 acgaactggt ttttcatcac ggggcactgg atgaactgcg acggcagatg gccgcgtaca
     4441 aagcgaaatc tccaaagatt gacgtggcga acttcaagca gctgacgggc gtcagccgaa
     4501 agtatgccat tccgttactc gaatatctcg acagggagcg cgtgacgcgc cgagtgggag
     4561 acttgcggga aattgtctga caaaggaccg cgtttttagg gacttctcaa tcgatgcttc
     4621 gaatccggaa agcaacagct catgatgccg cgctgctgtg gacgatgatc cgtgagcttg
     4681 cagccttcga gcacgagctc gaattcgtga cgatccgcca ggaggatctc gctcgggacg
     4741 gcttcggaga gaatccccgg tttcgcgcct ttattgctga gtgggatggc caagccgccg
     4801 ggtacgctct cttctttgac tactacacga catggacagg acgcggactt tttctggagg
     4861 acctattcgt ccgagagcaa tatcgagggc gccggatagg acagaccttg cttgcggcag
     4921 ttgcccgcat tgccgtcgac gaacggtgct acggaatcca ttgggaggtg ctcgactgga
     4981 acgagaaggc aatcgagctg tataaggccc tcggcgcgga gttccgcgat caatggcggc
     5041 ctgtgctatt gagcggtgaa gcgctgctga agctggcgga gaagtcgtcg tgacagcgcg
     5101 cgtgcgcatc atcggagcgg ggctggccgg ctcggaagcg gcttggcaat gtgcccggcg
     5161 tggcctcgag gtggagttgt tcgaaatgcg ccccgtgcga tcaaccccgg ctcatcagac
     5221 tgccggtttc gcagaactgg tttgctcgaa ctctttaaag tccgacagcg agaacaccgc
     5281 tccctggctg ctgaaagagg aaatgcgacg ggcgggatct ctgctgctgc gggtcgctcg
     5341 cgaatgcgcg gtccccgccg ggcacgctct ggcagtggac cgagtcgcct tttccgcaca
     5401 ggtcacagag aggctcttgc aggagccaca gatttcggtg cggcgcgagg aagtaactgc
     5461 gattgatgaa accagcggcg tgaccatcgt tgccaccggg cctcttacct ccgaggcctt
     5521 gacttccgag atcgcgcgct tgaccagcgc tttgcccggg gcggctctag cgaatccaca
     5581 cttgtatttc tacgattcca tcagcccgat tgtggaggcc gactccatcg acatgtcgcg
     5641 cgtctacctg gcagcgcgct atgacaaggg atcggcggac tacatcaact gccccatgac
     5701 ggcagaggaa tacaaccgtt tttaccatgc acttctgacc gcacaatcgg tcgaagagcg
     5761 cgattgggag aagctgaact atttcgaagg ctgtctgccg atcgaggaaa tcgcccgccg
     5821 cggtcgcgat actttgcgtt ttggcccgat gaagccggtc gggctgaaag atccgcgaac
     5881 gggcaagatg ccctacgcgg tggtgcaatt gcggcaagag aacctgcggg cggagtccta
     5941 caacttggtc ggatttcaaa atcatttgaa ctttggcgag caagcgcggg ttctgcgctt
     6001 gattcccggc ctggaaaatg cccgatttct gcgctacggc cagatccacc gcaataccta
     6061 catcaacgca cccactctgt tgcaggaaac gttgcagatg aaggcgcatc cgcgggtctt
     6121 ttttgcaggt cagatttgcg gtgtggaagg atacgttgag tccatcgcta ctggtctttt
     6181 ggcggccatc cacgccgccg cattggtttc cggggaggtc ttggcgcctg ccccacgaag
     6241 ctctgccttc gggtcgctca cgcactatgt gacccatgcc agccctgaga actttcaacc
     6301 cgcgaacatc acatttgatc tgctgcctct gctggacaag acggttcggg atcgaaagga
     6361 acggcactgt ctacagtgcc agagagcact tcaggacttc gatcgatggg tttccggggt
     6421 cggcgtaacc accttaaagg gttgaacccg ggacaatcag gccggattgg tctcgaaaag
     6481 tcctctattc tgccctgctt gccggcagct cgtcgccacc aagtccacac tgcctttttt
     6541 actgcaacaa aagtgagccc ggctccgtat ctaagagagg cggcggaagg attacgttcc
     6601 gtgtcccgcg gcgtccaacc ggcaatgacc gtgcgcgacc tcatttccga cgttctccgc
     6661 acattgtggg cgcacaagtt gcgtgcattt ctcaccatgt tcgggatcgc ctggggaatc
     6721 gtttcgatcg tgctgatggt ggctgccggt gagggattgc gcaaaggcca ggaggagcag
     6781 gcgcgaactc tgggtaagga tgtgatgatc gtctttcacg gccgcaccag cttgcaggca
     6841 ggcggcacgc acgccggtcg cgtggtgcac tgggaagatc ccgatgtgcc ggtagtgcag
     6901 gcagaatcgc ccgattgtga atacgcgatc ccggaactgg agcagaatac ggtccgcacc
     6961 cactccaatt tcaacaacgc cgctttcacg gtaacggggt cctatcctca gtttgcctac
     7021 attcgcagcc tcgacgtcgg cgcgggacgg ttctacgact gggatgatat gcgagaagcg
     7081 cgacgcgtcg cctttctcgg gtcggacgcg gcgaagcagc tttttcccgg gcgcaatccc
     7141 gtgggcgaca caatctacct gaacgatttc ccgtacaccg tgatcggagt catggcgaaa
     7201 aagaaacagg attccagcta cgacggctgg gatgtgaaca aggttttcct cccgtttgcc
     7261 gccatgcgcc gcgactttcc cgacaagcct cccgggacca ataccacgtt cgaccaactg
     7321 ctggtgacgc cgaaatcggt tgaccagcac gacgcctgca agcatgaagt gcgcgtcgcc
     7381 ctggcccgta tgcaccgcta cgaccccaac gacaaggaag cttgtccgat ttgggatacg
     7441 gtgcaggagg cgcaggcgtt cgaaaccatg accgacggaa tgaagttctt cctcggcgcg
     7501 gtagggatcg tcacgttgct gttgggcggg atcggcgtga tgaacgtgat gctggtcgcg
     7561 gtccgggagc gcacccgcga gatcggcgtg cgtaaggcca ttggagcgcc tgccagcacg
     7621 attctgaggc agttcttcat cgaggcgctg atcattgcac tgctcagtgg aggtatcggg
     7681 ctcggcgtgg catttggatt ttgcgccctg gtgaacctgc tccccatgcc tgactttttc
     7741 gccgggctgc tgcccagttg gagctcagga ctgatcgcgg ctggtcttct cggaacgatc
     7801 gcagtgggag cagcgctgta tccggcgcgg cgcgcggcgg cggtcgaccc gattgaggcg
     7861 ctgcgctacg aagccggagg atgagtccgg actgtttgat ctgtcttgcc caaagggttc
     7921 gatgctgatt gaaatcattc tcgaagcctg gatcgcgctt aagcgcaatg tcacgcgcag
     7981 cctgctcacc atgctgggta tcgtctgggg catcgcgacg gtgactttgc tgatcgccta
     8041 cggtagcagc ttccgcagca tccttgtgca cggcttcgac gcgttcggaa agagcgtggt
     8101 gatctgctgg ccacagcaga ccagcgagca accgggcggg cagcgcgccg gcaagaaggt
     8161 cgtcctcgag caagccgatc ttgacttggt gaaggagacc gcatcgctcg taaagcatgc
     8221 ttgcctggaa accgtggaac gtcctgggat cgcctacggc gatcgcattg caggaacctc
     8281 cccggtccgc ggagtttgcc cggagtatgg cgagatgcgg aatgaagttc catctgaggg
     8341 gcgttggatt aacgccgaag atgaactgga acgccggcgc gtcatctttc tcggcggacg
     8401 cctcaaggag caactcttca gcgggcgccc cgcagtcggc gaaaccgtgc aggtcgccgg
     8461 tgtgcgcttc accgtgattg gtgtcatggc gcggaagata cagctcagca actacttctc
     8521 cagcgacgac gattcgtcgt ggattcctta ttcagccgcc ggcgatctct ggaacacgcg
     8581 ttacgcagcc gtgatggtgt tcgaaccgat cgctccgcag ttcgaaaaaa aagccatggc
     8641 tcaggtactg gctgcgctgg cgacgcgaca gaacttctcg cccaccgatc ccaaagcgct
     8701 ccagatgttt ggcagggatg aatttcggcc aatcatcgat gcgctgacca ttgggctgca
     8761 ggtcctgctg acgttcatcg gcactttgac cctcggaatt ggcggggtcg gggtgatgaa
     8821 catcatgctc gtctctgtgg acgagcgcat tcgcgagatc gggctgcgcc gggcgctggg
     8881 cgcgcgcaag cgccacatta agctgcaatt cctggccgaa acattgcttc tgatgctcct
     8941 gggcggggcg atcggggtgc ttctgtcgta cgtggtcgca tggagcgtcg ggacgctgcc
     9001 gatgatgggg ccgctgtttg aggatgattc gggccagggc gacatccatc tccgcatttc
     9061 gatgttgacc gttatgctct cgaccgcagt gttgctcgtg gtgggcatca tcagcgggct
     9121 ggtgcccgcc atgcgtgcct cgaggcttga tccagttgaa gccctgcgct acgagtaaac
     9181 gctcaagcat cgcgacctcg acttccgggt ggaagtctag gcgcgtgtca gcggtgattc
     9241 caacccgcga acctggcctc gaattgattt gctccccatc cctcttccct gtaccttgct
     9301 gctgagcgtt acagccgagg tgcgttgtgc cgatttctgc ggtagacacg attacgctcg
     9361 cctttcagca cacgaaacgc caactgctgc agccgttccg cttctggcag tggaccagac
     9421 ttgcattggt tggcctgctg gcgggcgaga tgggctccgg cggaggaagt ttccgctttc
     9481 cttctagctt caatgtcccg caacaatccg gctcctcgcg gcactttctg gggtcgccgt
     9541 ttggctcggt cgatcctgcg ctgcttgtcg tactgatcgc cgtcatcgtc actgccgctt
     9601 tggtctttgg cgtcatcatg atgtacatca gcagcgtcat gcggttcatc ctgttcgaca
     9661 gcgtgctggc gaaggagtgc cacatccggc agggctggaa ccgcaggcaa ggtcccggct
     9721 ggcgctattt cctgtggcaa ctggtctaca tgctggccac gttcgccggt ctggtggtgc
     9781 tggtcgggat tccggcgggc ttagcctatg cgatgggatg gttcaagcag cccagccaac
     9841 atgttctcgg tctggtgctg gggggcgtag tggttttctt tctgttcgca gctttcatgg
     9901 tgactgcagc ggtggtgtac gtgctcacta aggattttgt cgtgccgcag atggcgctgg
     9961 agggcatcgg cgtcatggaa ggctggcgtc ggctctggcc catgttgcaa ggagaaaaga
    10021 gcggctatgc cggttacata ggcatgaaaa tcgtgctcgc gatcggggct ggcatcgtca
    10081 tcggcattgc ttcgttgatt ctggttttaa tgcttgccat tcccacggcg gcggtggttc
    10141 ttggtgcgat ctttgccggc aagagcgcgg gtctgacttg gaatgtcttc accatcacga
    10201 tcgcggtcgt ggtggggtgc gttctgttcg cacttttgat gtatgtactg gcgctgattt
    10261 ctgttccagc gatcgtcttc ttcccggcat actcgatcta tttcttcgcc ccgcgatatc
    10321 gcgcattgag cctggcgctg tatccgccgc caccccccct ggccgctccg cccgaaccac
    10381 cggagccacc accgcccctg gcgcctgcgc ctgcgacata gacatgatgc cgcccgcctc
    10441 agcattcgat gctacctgtt tttctgttgc tcatcgggag acttgagctt ggtgcccttt
    10501 ccgtgtggcc acgagaagcg ccggccaatg atgcatttca cgatcaggtg atcggtggac
    10561 cgggtagtgc cgactccaac tccaaagttg aactcccact ctggcccgaa gtcgacatcg
    10621 atggtaggga agaactgctg ctgctggtcc tgcaacggat cgaagcccgt gatcgggccg
    10681 taagaagcgt agtattccag cccacccgca attttcttcg tgaaatcgta gctgatctta
    10741 gcgttgggtg agaacaccac gccctgattg acgccggggc cgtgaaatga gcgatcgagc
    10801 gtgggattga agtcgagata ccacggcccg attttcttgt cgacgatggg gcgaatctcc
    10861 cacgtccagg tgtcggcaga gaatgcatgt cgctggtaac cgagttcgtt cgagacgctt
    10921 acccccaccg gccagtgcca gctatcgggc acacggacgc gcgggcggat gtgatcgcca
    10981 acccattgcc atccgtcgtt aggctggatg ctggaaaaca ggtaaaaccc cacctccgcc
    11041 caattgttga tgccttgcgt aatctccagc gtttcgtgct cagcgtggtt ggtgggaaga
    11101 attccatcct gcacggtctt gctgccgtca accgtgaagt tggaatgaat ctcgaccatg
    11161 gtagagcgcg gggcgaccgt gtcgtagctg tagacctgga tttcgtagtt atcctgggtg
    11221 aaggctgact ggctcagagc gggtagaacg acgctgacaa aaatcgcgaa gtgggggatt
    11281 gaaatcaggc gcgccattcg tgagtcctgg gccggcctca tccagcagcg cgaccgttcc
    11341 cgcctatgat gcatggtcgc agcggcgcaa ggcaacctcg cgttgtgact cgagagcaaa
    11401 gacctcggga acccttgctt cgatcgaggt gtctgatcct gcagacacgc gtgcttgaga
    11461 ggagcgaatc ccgatggctt tctccacgtc tggtgcaggc ggaggcgcgc gtgcggagat
    11521 caacgtcacc ccgctgatcg atgtgttgct gacgctgatc gtcatgttca tgctcgtggt
    11581 gtcaatggat aagcaatatc aggaagatgc gcagattccg cagccgggca agcagccgac
    11641 cgcgggagag gaacaatcga ggaccgtcgt aatcgaggtc gtctggacca gtcaggaccg
    11701 gcctccgagc gtaaagatca acaagcagga cattagttgg gaggacctgg gagggcagct
    11761 gcaaaagatc tacctgacgc gtgccgaaaa agtcgcgttc gtgcgcggcg atgccgatgt
    11821 ggattttcaa tatgtcgcgg acgtgattga tcgggcgcat cacgccggag tgcagcgcat
    11881 aggcctgctc accatggatc ggacggtgga gggcgagtag ataaggaagc ggttggctct
    11941 actctcgcgc ccttgccttg atccagatcg gtgtccccaa aaagccaaat gcctgccgaa
    12001 tctggttttc caaaaagcgc tgatacgaga agtggagctt gaccgcccgg ttggtgaaga
    12061 gaacgaacgt cgggggcgcc accgaggcct gcgtcatgta gagaattttt acccgctgcc
    12121 gcatcggcac cgacgcgcgg tcgaagtcga cgcgctcaag gaaccggttc atctccgagg
    12181 ttgtgatccg cttgcggcgc tctgttgcga cctgctccag aagcggaaat atgttctcag
    12241 cgcctttgcc gctcttggcg gaaacgaaaa ccacgggagc gtagttcaga aacttgagtt
    12301 catagagcaa gcgttgttca taggccgtgc ggtcggccgg ccgcttcgat tcgcgcatac
    12361 gctcggcctt ggtcggccgt gctgattttg ccttgccgct gatcaccaga tcccatttgt
    12421 tcacgacgat gatgaccgat cgtccgctct catgcgcata tcctgcgatg gcagcatcga
    12481 gctggctgac gccctcggta gcatcgatca cgagcagcgc aatgtcagcc gattccaggt
    12541 gcttgcgcgc catcacgacc gagagcttct cagccatcag atgcgtcttg cccttgcgcc
    12601 ggataccggc cgtatcgatg aagcgaaagc ggcgtccgtt
//
LOCUS       DASPUL010000352         7391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_62204, whole genome shotgun sequence.
ACCESSION   DASPUL010000352 DASPUL010000000
VERSION     DASPUL010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7391)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7391
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62204"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VEJ00_08490"
     CDS             <1..634
                     /locus_tag="VEJ00_08490"
                     /inference="COORDINATES: protein motif:HMM:NF020683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactose oxidase-like domain-containing
                     protein"
                     /protein_id="HXX01237.1"
                     /translation="IAPPGYYMLFILNNSGVPSVSTFVQVTAGAGQQQAMAMATQFAA
                     TAAPSTAAGLVQGQTTARTDHPAASPNAISPARANTPVDEGSQATRVTEAMARAGLAF
                     QGVWSGKFVSREPGAAPFDMKLVIQRDSHGQLSGTSTLTSKCLQHAHVQVTVVGSNFV
                     LSGNDEAGDNITVRGTLDKAGKVLNSTYILDGSATGRCETDTGTGTLSRQ"
     gene            643..1812
                     /locus_tag="VEJ00_08495"
     CDS             643..1812
                     /locus_tag="VEJ00_08495"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HXX01238.1"
                     /translation="MPARPEMTTSRRGFLRSLGAGVAAGAAIRWPLPALAGSVPFEPP
                     REAESGGLIRLDSNENAYGPLPKVAEAIKAAVSSSNRYPRMETRSLRERIAAFHHVKP
                     EQVVLGCGSTEILRVASFAFQGSGKQLIQAAPTFEAAEHYAQAAGSGVISIHLAPAYD
                     HDLERMLARVSTTTTLVYICNPNNPTASLTPRKDLEHFISRLPASTMVLIDEAYHHFA
                     GHPGMYTSFIDHPMEDDRVIVTRTFSKVYGLAGLRLGYAVAAPKLIEQMRRFATETNV
                     NAIVAQAAMAGLDDIDEVNEAVQRNDDDRQEFLNQALARQLKPIDSHANFAMMNTMHP
                     AEELIRHFREHNVLIGRHFPPLDNYIRVSFGRPEEMQAFWQAWDALPFAKEIMVH"
     gene            1854..1929
                     /locus_tag="VEJ00_08500"
     tRNA            1854..1929
                     /locus_tag="VEJ00_08500"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1888..1890,aa:Cys,seq:gca)
     gene            2216..5755
                     /locus_tag="VEJ00_08505"
     CDS             2216..5755
                     /locus_tag="VEJ00_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXX01239.1"
                     /translation="MSFCDETIKTGLIFKVAWKLFTPMEGRIHVKATTLRASTLITIV
                     CFSCMANVGCAGFSGSNGSKERTTGFPSSSSISGQISPVASAIGTIVSLKGVRGTVTT
                     FTDRFGRYSFGDLANGSYSVSASRGGVNLTPAAQFVRVENSNAAEIDFTAAQVAGSIW
                     GTITPAPNGGGVTVTLSGPTRATTATDSVGNFNFNDLPSGTYTVTPSKSGFSFSPPSR
                     SMTLASADFMGVSFSVSKVSSSPPPPPSTFGISGSISPAASGAGAIVTLGGAATASTT
                     ADSFGNFSFSGLPSGTYTLTPSKSGYSFGPSMQSATLTTANITAVSFAASKVTAPPQP
                     TFSISGVINPAANGAGTTVTLSGAASATATADNFGNFSFSGLSNGIYTLTPSKNGYTF
                     GPSAQVTSVASASVSGVMFTASKVIAPPPPTFSISGSISPAANGAGATVTLGGAASAS
                     TTADSMGNFSFSGLASGTFTLTPTKNGYTFGPSTQSTTVAAASVAGVNFAAAKVSVPP
                     PATFSISGSITPTANGSGVTVNLSGAASATTSTDSSGNYSFSGLSGGTYTLTPSKNGF
                     AFNPPSQVATVSDASASGVSFAASQQSTSGGGVTISPGDDIPSIVAASPAGTTFVINP
                     GTYRLTRPIIPKTGDSFVGQAACAPPASSCPAIISGSTAIGQLATFDGTNYRATGQTQ
                     QNQQGTGNICDGGWSGCIYPEDLFFDGVPYQHLNSATLPAIGPGQWSFDYANHTIYFH
                     DNPSGHTVETSVVNNAFGGSANNVTIQYLTVEEFASMYPVGAIGVSQGNNALTQSTNW
                     TIENSEITLNHGYGVRVNYGTQILNNYIHDNGQLGIGGGIGTHSNPSTQSVDSAVLIQ
                     GNTINHNDYAHFSAGWGSGGFKVGSTSGIVLRGNTIQHNEGAGIHFDMNSQSEWVDGN
                     TITDNTDADGLEMEIGYGQSTFRNNVVLRNGAQVNNSNYTYQIAVRSSSGVNAYCNIM
                     EVSSGPGINGWGVDATNRGYSSYPPYQYLVSTGNSFHHNTVLWDAGANGKVGFHQNDV
                     NQPNFFANNSAPDFNSYHLPSLGAADFQYNSGGSQNSGSQVFPIYQAAGADVHGSADS
                     NYSSGYPTVAITSPGDQSSVSSGAAVVASASDQSGISKVEFYVDWVLQATSTSSPYSF
                     TWTNGASGLHTVAAMAYSNAGVSACSAVTLNQQ"
     gene            complement(5880..7151)
                     /locus_tag="VEJ00_08510"
     CDS             complement(5880..7151)
                     /locus_tag="VEJ00_08510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX01240.1"
                     /translation="MSQTNDGTIVSVEVCSHFPDIMGHPELKVQSQWGTADLVDEIAE
                     EWRELCQEGSCNTPFYLPEWIAAAIRAFAGQQRFLLVTVRDEARLRGVLPLLEIKESV
                     AGFPCTRLRSASRIPRFEFVHGEGPDVDDVVRAAWRHLRDLPSWDAIELVNVPEGGGA
                     ERLLAVAKEDAFPTCRLEYAKSPYIDLKGLRSGDDFSRFGQSSRFRYHLRQGWREISK
                     RGAVRSQREEKLRPDALQDFYRLEQSGWKGKEGTAITSKPELQRFFDAVAHAAEQFGY
                     LSMYSLKQGDTTIAAHLGLTYAERYYPIKVAYDEHFSKYGPGHLIIGQVLHDCVERGL
                     TEFDCLGEWTEAKAKWTSKSRLHTSCSIFRTSAGGRILWAKTRLQDQWNQTRRGLKPL
                     VASARAYVAQLKKRGSRPPSGRAGSKEADRE"
     gene            complement(7209..>7391)
                     /locus_tag="VEJ00_08515"
     CDS             complement(7209..>7391)
                     /locus_tag="VEJ00_08515"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXX01241.1"
                     /translation="DKSGIRKVEFYVDWTLQATVNSAPYNFNWTNGNSGAHTVAAMAY
                     SSAGIHACYAVTLTQQ"
BASE COUNT         1542 a         2363 c         2052 g         1434 t
ORIGIN      
        1 cattgctccg ccgggatatt acatgctgtt tatcctgaac aattccggcg tgccctcggt
       61 gtcgaccttt gttcaggtaa ccgcaggtgc aggccagcag caagccatgg cgatggccac
      121 tcagtttgca gcgaccgcgg ctccgtcgac tgcggccgga ctcgtgcagg ggcaaacaac
      181 tgcccgtacc gatcatcccg ctgccagccc taatgcgatc agcccagctc gcgccaacac
      241 gccagtcgac gaaggcagcc aagctacgcg tgtgactgag gcgatggccc gggccgggct
      301 cgctttccag ggggtctggt ccggcaaatt tgtctccagg gagcccggag cagctccctt
      361 cgacatgaag ctggtcattc agcgcgactc gcacggacaa ctctccggca cgtccacgct
      421 gacttcgaag tgcttgcagc acgcccatgt gcaggtcacg gttgtgggtt cgaacttcgt
      481 cctgtcgggc aacgacgaag ccggcgataa cattacggtg cgaggcaccc tcgacaaggc
      541 aggcaaggtt ctgaactcca cctatattct cgacggcagc gccaccggca ggtgcgaaac
      601 cgatactgga accggcacct tgtctcgaca atagagctct caatgccggc tcgacccgaa
      661 atgaccacgt cgcgtagagg ctttcttcgt tccctcggtg ctggagtggc ggccggtgcc
      721 gccattcgct ggccccttcc ggcgcttgcc ggttcagtcc ccttcgagcc accgcgtgag
      781 gccgaaagcg gcggtctgat ccggctggac agcaatgaga atgcctacgg ccccctcccg
      841 aaggtggccg aggcgattaa ggcagcggtc agcagttcca accgatatcc gcggatggaa
      901 acccgatcgc tacgggagcg cattgccgct ttccaccatg tgaagccgga gcaggttgtg
      961 ctgggttgcg gctcgaccga aattcttcgc gtggcctctt tcgcttttca ggggagcggg
     1021 aagcagctga tccaggcggc gccgactttc gaagccgccg agcactatgc ccaggcagcg
     1081 ggttcaggtg tcatctccat ccacctcgcc ccggcttacg atcacgatct tgagcgcatg
     1141 ctggcgcggg tgtcgacgac gacgacgctg gtctatatct gtaacccgaa caatccgact
     1201 gcgtcactca cgccgcgcaa ggatctggag catttcatca gcaggcttcc cgcttccacg
     1261 atggtgctga tcgacgaggc gtaccatcac tttgccggcc atccgggaat gtacacctcg
     1321 ttcattgacc atccgatgga agacgatcga gttatcgtga cccggacatt ctcgaaggtc
     1381 tacggtctgg cgggcttaag gctaggctac gccgtagcgg cgcccaagct gattgagcaa
     1441 atgcggcgat tcgccactga gaccaatgtc aacgcgatcg tcgcccaagc cgctatggcg
     1501 ggcctcgacg acatcgacga agtgaacgaa gcggttcagc gaaatgatga cgatcgtcag
     1561 gagttcctta atcaggctct ggctcgtcaa ctgaagccca ttgattcgca tgctaacttc
     1621 gccatgatga acacaatgca tccagccgag gaactgatcc ggcatttccg cgaacacaac
     1681 gttctgatcg gacgccactt ccctcccctg gacaactaca tccgggtttc gttcggccgc
     1741 ccggaggaaa tgcaggcgtt ttggcaggcg tgggatgcac tgccatttgc caaagagatc
     1801 atggttcact gagatagtgg cccactgagc tgtaggccta tactcccccg accggcgcgg
     1861 tacccaagtt ggctaaggga agggcttgca aagtcctggt cggcggttcg attccgtccc
     1921 gcgcctccaa tcttttcccg catcaagcgc ccttcactag tcccgcttcg cacttccggg
     1981 cgatagccct cgaaaccacc tgccatggga cggccgcttc cgcacttgcg cgatcctgcc
     2041 cgctatcgtc ttccggtcct cgccaaaccc aatattcgtc cgccttccaa cgcgcactcc
     2101 ccggccgatc ggctaaccca gagctcaagt caccaagcag cagatgaatt cgttccctca
     2161 cacgtcggac atgtcccgat gcttggccag gtgctagtcc tccgttatct ggttcatgtc
     2221 cttctgcgat gaaaccatca aaaccggtct gattttcaag gtggcgtgga agctcttcac
     2281 ccccatggag ggtagaattc acgtgaaagc cacgactctt cgagccagca cactgattac
     2341 tattgtgtgc ttctcctgca tggccaacgt cggctgtgcc ggtttttcag gttctaatgg
     2401 cagcaaggaa cgcacgaccg gctttcccag ctcctcgagc atctcgggcc agatcagccc
     2461 tgtcgcgagc gccattggaa cgatcgtaag ccttaaaggt gtgaggggaa cggtcaccac
     2521 cttcaccgac cggttcggtc gctactcctt cggggacctt gccaacggct cctactcggt
     2581 gagcgcgagc cgtgggggcg tgaacttaac cccggctgcg cagttcgtga gggtcgagaa
     2641 ctccaatgcc gctgaaatcg atttcactgc tgcgcaggtc gcaggaagca tctggggtac
     2701 catcaccccg gcaccgaatg ggggtggagt aacggtcacc cttagcggtc cgacccgcgc
     2761 caccaccgct accgacagtg ttgggaactt caacttcaac gatttgcctt ccggcactta
     2821 cacagtaact ccgagcaaga gcggattcag cttcagtcca cccagccgct ccatgacgct
     2881 cgcctcggct gacttcatgg gtgtgagctt ctctgtttcc aaggtctcgt cgtcgccgcc
     2941 gccgccccca tcaacgttcg gcatctcagg cagcattagc ccagcggcga gcggagccgg
     3001 cgcaattgtc accctcggag gcgccgccac cgccagcaca acggcggaca gtttcggtaa
     3061 tttcagcttt agcggcttac caagcggtac ctacacgctg acgcccagca agagcggata
     3121 cagctttggt ccgtcgatgc aaagcgccac cctcacgacc gcgaacatca ctgcagtaag
     3181 cttcgcggcc tccaaagtga ccgcgccacc gcagcccacg ttcagcattt cgggcgtcat
     3241 caacccggct gcgaacggag ccggcacaac cgtgactctg agcggtgcgg cgagcgccac
     3301 cgcgacagcc gacaacttcg gcaacttcag tttcagcggg ctgtccaacg gaatctacac
     3361 tctcactccc agtaagaacg ggtacacttt tggcccttcc gcgcaagtca caagcgtcgc
     3421 cagcgccagc gtttcaggag taatgttcac ggcgtcaaaa gtcatagcac ctccgccgcc
     3481 gaccttcagc atctcaggca gcatcagccc ggcggcaaac ggagccggcg caaccgttac
     3541 tctcggaggc gccgccagcg cgtcgacgac tgccgacagc atgggcaact tcagtttcag
     3601 cggactggcc agcggcacct ttactctcac accgaccaag aatggataca cctttggccc
     3661 gtcgacgcaa agcacgactg tcgctgccgc aagcgtcgcg ggcgtgaact ttgccgccgc
     3721 caaagtctcg gtgccaccgc cggcaacgtt cagcatctcg ggaagcatca caccgaccgc
     3781 caacggaagc ggcgtcaccg tgaacctcag cggtgcagcc agcgcaacga ccagcaccga
     3841 cagttccgga aactacagct tcagcggatt gtccggcggc acctacacgc tgactcccag
     3901 caagaacggt ttcgcgttca acccacctag ccaggtcgca accgtcagcg acgccagtgc
     3961 tagcggtgtg agcttcgcgg cctcgcagca gagcacttcc ggcggcggtg tgacgatcag
     4021 ccccggggac gatattccca gcatcgtcgc agcctctccg gccggcacga ccttcgtgat
     4081 caatccagga acttaccggc tgacgcggcc gatcattccc aagactggcg acagcttcgt
     4141 cggccaggcc gcctgtgcgc cgccagcatc ttcctgtccg gctatcatca gcggctcgac
     4201 cgcaatcggc cagctggcga cattcgatgg aacgaactac agagcgaccg ggcaaacgca
     4261 gcaaaaccag caaggcaccg gtaacatttg cgatggcggc tggtccggct gcatttaccc
     4321 tgaagacttg ttcttcgacg gcgtaccgta ccagcatctg aactcagcga ccttgccggc
     4381 catcggcccg ggccagtggt cgttcgacta tgcgaatcac accatctact ttcacgacaa
     4441 cccgtccggg cacacggttg agaccagcgt ggtgaacaac gccttcggcg ggtcggccaa
     4501 taacgtcacc atccagtacc tgacggtcga ggaattcgcc tccatgtacc ccgtaggcgc
     4561 gatcggcgtt tcgcaaggca acaatgccct gacccagagc actaactgga cgattgagaa
     4621 cagcgagatc acactgaatc acggctatgg cgtgcgggtg aactatggca cgcagatcct
     4681 taacaactac atccacgaca acggacagtt ggggatcggg ggcggcatag gaacacattc
     4741 caatccctcg acccaatccg tcgactcggc cgtcttgatt caggggaaca cgatcaacca
     4801 caatgactat gcccacttca gcgccggctg gggatcagga ggcttcaagg tcgggtctac
     4861 ttctggaatc gttctgcgag gcaacacaat tcagcacaac gagggcgccg gcatccattt
     4921 tgacatgaac agccagagcg aatgggtgga cggcaatacc attaccgaca acactgatgc
     4981 cgacgggctg gaaatggaga tcggatatgg acagtcgaca ttccgcaaca acgttgtcct
     5041 gcggaacggc gcccaggtca acaactccaa ctacacctac cagatcgcgg tgcgctccag
     5101 ctcgggcgtg aacgcctact gcaacatcat ggaggtctcg agcgggcctg gcatcaacgg
     5161 ctggggtgtc gatgccacca atcgcggcta cagcagctat ccgccttacc agtacctcgt
     5221 cagcaccggc aattctttcc accacaacac agtgctctgg gatgcgggag cgaacgggaa
     5281 ggtcggattc caccagaacg acgtcaatca acccaatttc tttgccaata actccgcgcc
     5341 cgatttcaat tcttaccatc tgcccagcct gggcgctgcc gattttcagt acaacagcgg
     5401 ggggtctcag aacagcggat cgcaggtctt ccccatatat caggcggcag gggctgatgt
     5461 tcacggcagc gctgactcga actacagcag cggatacccg acagtggcga tcacatcgcc
     5521 cggcgatcag tcgtcggtga gcagcggagc tgcagtggtg gcctcggcgt cagaccagag
     5581 cggcatcagc aaggtagagt tctacgtgga ctgggtgctg caagcgacct caaccagcag
     5641 cccatacagc ttcacatgga ccaatggagc ctcgggactg catactgtag cggcgatggc
     5701 ctacagcaac gcgggggtca gcgcttgctc ggcggtaacg ctgaaccagc aatagtcagt
     5761 ctggtcatac ccgcgagcag cgcagaaaac tggcgcataa ccggaaataa aaaaggccct
     5821 tccaaaatga agggcctttt gctcgcgcag ttcgaacgaa cttgtggcaa acccctcgct
     5881 tactctctat cagcctcctt gctgcctgcc ctcccggacg gtggtcggga cccgcgtttt
     5941 ttcagctgcg ccacgtaggc gcgcgcggaa gcaaccaacg gcttaagccc ccgtcgcgtc
     6001 tggttccact ggtcttgcaa tctggttttt gcccacagaa ttcgcccacc cgcgctcgtg
     6061 cgaaagatac tgcaggatgt gtgcaagcgg gacttcgagg tccatttcgc cttcgcttcg
     6121 gtccactccc cgagacaatc gaattcggtg agcccgcgct cgacgcaatc gtgcaggacc
     6181 tgtccgataa tcaggtgccc ggggccatat ttgctgaaat gctcgtcgta ggccaccttg
     6241 atcgggtagt agcgttcggc atatgtcaag ccaaggtgtg cggcaatggt cgtgtcgccc
     6301 tgcttcaggg aatacatcga gagatatcca aattgctcgg cggcatgcgc cacagcatcg
     6361 aagaatcgct gcagctccgg cttgctggtg atggccgtgc cttccttgcc tttccatccg
     6421 ctctgttcga ggcgataaaa gtcttgcagc gcgtcagggc gtaatttctc ctcgcgctgc
     6481 gaccggacgg ctcctcgctt gcttatctcg cgccagccct gacgcaggtg atagcggaag
     6541 cgagagctct ggccgaagcg agagaaatca tcgcccgacc gaagtccctt gaggtcgata
     6601 tacggactct tcgcgtattc caagcgacag gtaggaaatg catcttcctt ggcaaccgcc
     6661 agaaggcgct cggcgcctcc gccctcgggc acgttcacca gctcgattgc atcccagctc
     6721 ggcagatcac gaagatgcct ccatgccgcc cgtaccacgt cgtcgacgtc gggtccctcc
     6781 ccatgcacaa attcaaagcg tggaatccgg ctggcgctgc gcagacgcgt gcacggaaag
     6841 cctgcgactg attccttgat ctccaacaag ggcagcaccc cccgcagacg ggcttcgtct
     6901 cgtactgtga ccaacaggaa tcgctgctgt ccggcgaagg cccgaattgc ggccgcgatc
     6961 cactccggaa gatagaaggg agtattgcac gacccttcct ggcacagctc ccgccattcc
     7021 tcggcgatct cgtccacgag gtcggctgtt ccccactgac tttgtacctt cagttccgga
     7081 tgacccataa tgtcaggaaa gtgcgaacac acttcgacac tgactatcgt accgtcgttg
     7141 gtctgcgaca aggcgttgat tctggaagga tcgtattgca aaggacgtgg agtttcctcg
     7201 aaacgcggtt actgttgagt caaggtcacg gcgtagcagg catgaatgcc cgcactgctg
     7261 taggccatgg cggcaacggt atgggctccg ctgtttccgt tggtccagtt gaagttgtaa
     7321 ggcgcactgt tgacggtcgc ctgcaaagtc cagtccacat agaactcgac cttgcgaatt
     7381 ccacttttgt c
//
LOCUS       DASPUL010000353         4513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1162052, whole genome shotgun sequence.
ACCESSION   DASPUL010000353 DASPUL010000000
VERSION     DASPUL010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162052"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VEJ00_08520"
     CDS             <1..993
                     /locus_tag="VEJ00_08520"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXX01242.1"
                     /translation="PVVDTPDWVGWLRAPERGAVVNVFPIAAATRASRGGTLTDFSAL
                     QRAGAVSVTDDGKPILEDETMRMALRLGAELNLPVVQHAEDTRLTENCSMHLGPTSFR
                     LGLRGMPAAAESAIVDRDVTLAQQIRESRLHVAHLSTSDALKAVRRGKRAKARVTCEV
                     TPHHFTLIDENVGEYDTNFKMNPPLRSATDREAILVALADGTVDAIATDHAPHAAHEK
                     QVEFERASFGITGLETALALAITKLHREHRIPLTRIVELFTAGPARVFDLRGRGSLAK
                     GSYADVTVFDPKKRWRFEASKSRSLSRNTPFDGWQLIGKVIATIVDGKIVHRSD"
     gene            complement(1053..2270)
                     /locus_tag="VEJ00_08525"
     CDS             complement(1053..2270)
                     /locus_tag="VEJ00_08525"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HXX01243.1"
                     /translation="MTTATRINPLVYLTNQLDELKAKGTHFKLRVLEDEQAPVCTFDG
                     KKVINLASNNYLGLTTHPRLREAALEATRKFGVGSGAVRTIAGTMKIHMELEEKIARF
                     KNVEACVVFQSGFAANAGTVSAVLGKDDFIISDALNHASIIDGARLSKAKILVFRHKD
                     IAHAEEQLASVQNESGKKLLITDGVFSMDGDIGPLPALCDLAEKYGAIMMVDDAHASG
                     VLGRNGRGTIDHFQMHGRVDIQVGTLSKAIGALGGYVCGTRDLIDFLYHRARPFLFST
                     SHPPSVAATCIAAFDVLESEPERMEELWENTRFWKKELGLLGFDIGGRTTPASETPIT
                     PIIIGDGKLTMDFSRELFKEGVLGTGIAFPTVPEGKARVRTIMTATHTREELEQALEV
                     LKRVGQRMGILSN"
     gene            complement(2362..3414)
                     /gene="tdh"
                     /locus_tag="VEJ00_08530"
     CDS             complement(2362..3414)
                     /gene="tdh"
                     /locus_tag="VEJ00_08530"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HXX01244.1"
                     /translation="MATTMIAVVKPESAPGADVREVKIPAFGRTDVLVKVKVASICGT
                     DLHIYNWDRWAQNRIHPPLIPGHEFCGEVVAYGDEVTSVKEGDFVSAEMHVACGKCLQ
                     CRTGEAHICQNVKIIGVDANGAFAEYVVIPESNIWKLDPAIPQEYASILDPLGNAVHT
                     VLAGEIAAKSVAITGCGPIGLFSIAVARAVGATSVFAIEVNEHRASIARAMKADYVLN
                     PAKEDVEAVVMEKTGGLGVDVVLEMAGHPDSIRTAFDIVRRGGRISLLGLTSKPVQLN
                     FSEDIIFKGITVQGINGRRMYQTWYQMTALLKAGKLDLHPAITDRIPMHDFGKAMERL
                     KTGEASKILVYPNGVR"
     gene            complement(3652..4218)
                     /locus_tag="VEJ00_08535"
     CDS             complement(3652..4218)
                     /locus_tag="VEJ00_08535"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HXX01245.1"
                     /translation="MSDAAKHYLEEARRQLRGHKRMGEGAMAQLRDEDFFVTLDPESN
                     SVAILVKHLAGNMRSRFTDFLTSDGEKPDRFRDREFEVTGTTTRTDVMKWWEEGWACV
                     FGAIDPLKPEDVMRTITIRGEPHTVLQAINRQIAHYAQHLGQIVLLAKHLRSSEWKTL
                     SIPRGKSEEFNDHKVPAQKYERGVPKEK"
     gene            complement(4220..>4513)
                     /locus_tag="VEJ00_08540"
     CDS             complement(4220..>4513)
                     /locus_tag="VEJ00_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01246.1"
                     /translation="DNWRVDGAKITSEIPKLEAALERTVLILEHWFFRGSRSPERTFW
                     EEFGELRRYLETQVRPGDLLYFWEFDKCCTNDNALASGKYPDDQGRVPLGGAY"
BASE COUNT          886 a         1370 c         1320 g          937 t
ORIGIN      
        1 cccgtggttg acacgccgga ctgggttggc tggttgcgag ctccggagcg cggagccgtg
       61 gtcaacgtgt ttcctatcgc ggccgccacg cgcgccagcc gcggcggaac gctgaccgac
      121 ttcagcgctc tgcagcgtgc cggagccgtc tccgttaccg acgatggcaa accgatcctg
      181 gaagacgaaa ccatgcgcat ggcgctgcgc cttggcgccg aactgaatct ccccgtcgtg
      241 cagcatgcgg aagacacgcg cctgactgaa aattgctcga tgcacctggg gccaacgagt
      301 ttccggctgg gcctacgtgg catgccggct gcggcagaat cggcgattgt cgaccgcgac
      361 gtgaccctcg cgcagcagat tcgagaatcg cgtttgcacg tggcgcatct ctccacatcc
      421 gatgcgctga aagcggtgcg ccgcggcaag cgcgccaaag cccgtgtgac gtgtgaagtc
      481 actccacatc acttcacgct catcgatgag aacgtcggcg agtacgacac caacttcaag
      541 atgaacccgc cgctgcgctc ggccacagac cgcgaagcga ttttagtggc gctggccgac
      601 ggtacggttg acgcgatcgc gacggatcat gcgccgcacg ccgcccacga gaagcaggtc
      661 gaattcgagc gcgcgtcttt tgggattact ggcctggaaa cggccctggc gctggcgatc
      721 accaaactcc atcgcgaaca ccgaattcct ttaactcgca ttgtcgaact gtttaccgca
      781 ggcccagcgc gcgtgttcga tctgcgtggg cgcggctcgc tggcgaaagg cagctacgcg
      841 gatgtgacgg ttttcgatcc caagaaacgc tggaggtttg aagcgtcgaa gtcgcgctca
      901 ctctcccgca acacgccctt cgacggctgg cagttaatag ggaaagtgat cgcgacgatc
      961 gtggatggaa agattgtgca ccggtcagac taaggaccat gtagcgacag ccgccctcgg
     1021 ctgtcgggcc gagcggaact cggcagttgc tttcagttgc tcaagatccc cattcgttgc
     1081 ccgacgcgct ttaagacctc cagcgcctgc tccagttctt ctctcgtatg cgtagcggtc
     1141 atgatggtgc gcacgcgcgc ttttccctcc ggcaccgtcg ggaatgcgat tcccgtgccc
     1201 agcacgcctt ccttgaacag ctcgcgcgag aagtccatcg tgagtttgcc gtcgccgatg
     1261 atgatgggcg tgatcggcgt ctcgctggcc ggcgtggtgc gacctccgat gtcgaatcca
     1321 agcagaccca actctttctt ccagaaacgc gtgttttccc agagctcttc catgcgctcg
     1381 ggctcgcttt cgagaacatc gaaagctgca atgcaggttg ccgcgaccga cggcggatgc
     1441 gacgtcgaaa acaggaacgg ccgcgcccgg tgataaagaa agtcaatgag atcgcgtgtg
     1501 ccacagacat atccgcccag cgcgccaatc gccttcgaca gcgtccccac ttgaatgtcg
     1561 acccgcccgt gcatctggaa gtgatcgatc gtgccacgcc cgttgcgccc cagcacgccc
     1621 gaagcgtgcg cgtcgtcgac catcatgatg gcgccgtatt tttcggctag atcgcagagc
     1681 gcgggcagcg ggccgatgtc gccatccatg gagaagacgc cgtcggtgat cagcagtttc
     1741 ttaccggact cgttctgaac gctcgctaac tgttcttctg catgcgcgat gtctttgtgg
     1801 cggaagacca gaatctttgc cttcgacagc cgcgcgccgt cgatgatcga ggcgtgattc
     1861 aacgcgtcgg aaatgatgaa atcgtctttg cccaagacgg ccgacacggt accagcattc
     1921 gctgcgaatc ccgactgaaa gactacgcag gcctccacgt tcttgaagcg cgcgatcttc
     1981 tcttccagct ccatgtggat cttcatcgtg ccggcgatcg tgcgcacggc accggatccc
     2041 acgccgaact tgcgcgtggc ttccagcgcg gcctcgcgca atctgggatg cgtggtcagt
     2101 cccagataat tgttcgacgc caggttgata accttcttgc cgtcaaacgt gcaaacgggc
     2161 gcctgctcgt cttccagcac gcgcagcttg aagtgcgtgc ccttggcctt gagttcgtcg
     2221 agttggttgg tgagataaac gagcggattg attcgggttg cagtagtcat aggaacctca
     2281 ccacggatca gcagggattc acacgggtca gagagagaag cattatccgg gtagatccgt
     2341 gaaaatccgt ggcaaaggaa gttacctcac cccgttcgga tacaccagaa tcttgctggc
     2401 ctcgccggtc ttcagccgct ccatggcctt gccgaagtcg tgcatcggga tgcggtcggt
     2461 gattgccgga tgcagatcga gcttgcccgc cttcagcagc gccgtcatct gataccaggt
     2521 ctgatacatg cggcggccgt tgatgccctg caccgtgatc cctttaaaaa tgatgtcttc
     2581 agaaaaattc agttggacgg gctttgaggt aaggccgaga agggaaatcc ggccgccacg
     2641 acgcacgatg tcgaaggctg ttcgaatcga atcgggatgg ccggccattt cgagcacgac
     2701 atcgacgccc agcccaccgg tcttttccat cacgacggct tcgacatctt ctttggcggg
     2761 attgagcaca tagtccgcct tcatggcacg cgcgatgctg gcgcggtgct cgttgacctc
     2821 gatcgcgaaa accgaagttg ctcctacggc cctggccact gcaatggaaa atagacctat
     2881 aggtccgcat cccgtgatcg caacgctctt ggcggcaatt tcccccgcca gaaccgtgtg
     2941 aaccgcgttc cccagcggat cgaggatgga cgcgtactcc tgcggaatcg ccggatcgag
     3001 tttccagata ttcgactccg ggatgaccac gtattcggcg aaggcgccat tggcatcgac
     3061 gccaatgatc ttcacgttct ggcagatatg cgcctcgccg gtgcggcact gcaggcactt
     3121 gccgcaggcc acgtgcatct cggcggaaac gaaatcgcct tccttcacgc tggtgacttc
     3181 gtctccgtaa gccaccactt cgccacagaa ttcatgcccg ggaatcagcg gcgggtggat
     3241 gcggttctgc gcccagcggt cccagttgta aatgtgcagg tcggtgccgc agatggacgc
     3301 caccttgacc tttaccagca cgtcggtgcg cccgaaagcg ggaatcttca cttcgcgaac
     3361 gtcggcgccg ggagcggact ccggcttgac caccgctatc attgttgtcg ccatgattgc
     3421 caccttgaaa ctgataggaa cgaaagggcc caaacacaaa acgtatgatt ctagcaccgg
     3481 gagcggcttg gccagcgccg tgccagtact tccattttcc tctaccggcg cggtcgttac
     3541 aatcactgga atgaggagta acgcggcggg gcgagcggca ctgctattgg ggttgtacgc
     3601 gtggagcgac gcactcctcc acacttccaa gacatgccgt gccctgacgg atcacttttc
     3661 cttgggaacg ccgcgctcgt acttctgcgc cgggactttg tggtcgttga actcctccga
     3721 tttgccacgg ggaatgctca gcgtcttcca ctcgctcgag cgcaggtgtt tggccagaag
     3781 cacgatctgc ccgagatgct gggcgtagtg cgcaatctgg cggttgatgg cctgcaacac
     3841 ggtgtgtggt tcgccacgaa tggtgatggt gcgcataacg tcttctggct ttagcggatc
     3901 gatcgccccg aaaacacagg cccagccctc ctcccaccac ttcataacgt cggtgcgtgt
     3961 tgtcgtgccg gtgacttcaa attcgcggtc gcgaaaccgg tctggcttct cgccgtcaga
     4021 cgtgaggaag tcggtgaagc gcgagcgcat gttgccggcg agatgtttga cgagaatggc
     4081 gacagaattc gattcgggat cgagcgtaac gaagaaatcc tcatcgcgga gctgcgccat
     4141 tgcgccttcc cccatgcgct tgtgcccgcg cagttggcgg cgggcttcct caaggtagtg
     4201 cttggcagca tcactcatat cagtacgctc ccccaagagg aacacgtcct tgatcgtcag
     4261 gatactttcc tgaggcaagt gcgttgtcgt ttgtgcagca tttgtcgaat tcccagaaat
     4321 acaatagatc gccgggacga acctgggttt cgaggtatcg tcgcagttct ccgaactcct
     4381 cccagaaggt ccgttcgggt gatctagagc cccgaaaaaa ccagtgctcg agaatcaaaa
     4441 cggtcctctc aagcgcagcc tcaagcttgg ggatttcaga cgtgatcttt gcgccgtcca
     4501 cgcgccagtt gtc
//
LOCUS       DASPUL010000354         5862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_745430, whole genome shotgun sequence.
ACCESSION   DASPUL010000354 DASPUL010000000
VERSION     DASPUL010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745430"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VEJ00_08545"
     CDS             <1..817
                     /locus_tag="VEJ00_08545"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HXX01247.1"
                     /translation="QLARELGQPVVIDNKGGAGGILGTMEVVRAAPDGYTLLMVSPSV
                     TAANPAINPNAGYDPVTDLTAITIVAAGPTILAVRAGFPAKNYQEFIAEVKKNPDRYT
                     YATPGVGGILHLEVEYFKSLTGTSIRHVPFRGAGPALVAVTSGQVDMMEDALPSSLPS
                     INAGKLVPLVVSAPTRMKEIPNVPTFAEVGLPQLNHMSHWGILGPKGLPQEVIDKVNA
                     AVRKAVNDPTVKKRIEDSGGFVVASSPQQFSADIKDLYTQLKKVVADRKLTTE"
     gene            complement(1016..1786)
                     /locus_tag="VEJ00_08550"
     CDS             complement(1016..1786)
                     /locus_tag="VEJ00_08550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX01248.1"
                     /translation="MTMSNNVPGAPAPELPPPANRLRGRRIVITGAASGIGRTTALLF
                     AQEGASLALFDRDASGLALTTRQTGGHPLEVDISNEDQISKAAESASTRLGGIDGIVN
                     AAGIMPHGSVAETPVAVWRRTLDVNLTGTYLVIRSCLPWMEQESAATIVNIASAAGLL
                     PNAPGLTAYAASKGGVVNLGRALAAELAPKIRVNTVCPGMVDTPMADGFRGNTQNYAL
                     KRMADPAEIAQVILFLTSAQSSYVTGAALAADGGRSFH"
     gene            complement(1795..3282)
                     /locus_tag="VEJ00_08555"
     CDS             complement(1795..3282)
                     /locus_tag="VEJ00_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HXX01249.1"
                     /translation="MKTYQHWINGKYVAPVSGQSLETFDPYQGVAWAKIARGTAEDAN
                     RAVAAAHDAMYKGPWCTMTASERGKILRRIGDLVGETRHAQNLAEIESRDNGKILAEM
                     HGQLKSLPDCWYYFAGLADKIEGTVIPVDKADVLAMTLHEPVGVVVAITAWNSPLQFF
                     ALKCAPALAAGCSVVLKPSEFASVSSLEFAELTKEAGLPDGVINVVTGLGQEIGSALV
                     EHRDVAKITFTGSDVTGARIYEAAARGLKRVTMELGGKSPNIVFEDADLDLACVGAVA
                     GIFGATGQMCTAGSRLLVQNSIRQQFTERLAAMAKNLKLGDPFSADTQIGPISTKPQY
                     EKVLHYIELAKAEGARCVLGGSPASGEGLKGGMFVQPTIFTDVTNRMRIAQEEVFGPV
                     LSVIGFDTEEDAIRIGNDVIYGLAAGVWTRDIGRAVRMSKALRAGMVWVNTYRAYSYM
                     VPIGGMKHSGLGRESGIEAIKEYFETKSVMISSGAHAPANPFVQR"
     gene            complement(3325..4923)
                     /locus_tag="VEJ00_08560"
     CDS             complement(3325..4923)
                     /locus_tag="VEJ00_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HXX01250.1"
                     /translation="MTIEDFDYIVIGAGSAGCGVAARLSESRHHTVLVLEAGGRDSNP
                     WIHIPIGFSKTFFDETLNWCFSTEASSQLHARKLFAPCGKVLGGSSSINGQVYVRGHR
                     DDFDDWARAGNSGWGYDDVLPFFRKSEDQQRGANHYHGSGGPLSVSDLVDFSPLGQAF
                     VESGVAGGLDRNDDFNGPSQEGTGFFQVTVRNGRRASSAAAFLRDAERRSNVCLKTNA
                     EVERLMIESGRVTEVRYGRGGESLVARARRAVVLCAGALNSPAILQRSGIGRGEWLQA
                     AGIAVRHELPGVGANLQDHVQARLALRSRRHATLNTQTRQPLRLVGMGLKYALFRRGP
                     LTFAGGQCGAFVRSHPKLDRSDTLLILMPFSSADYRKGLDEFPGFTITSCLMRPESRG
                     TVRVRSPAAKDPPVIQPNYLEAESDRRAMVAGLRAARHVAATKPLRDEIEREERPGLN
                     VNSDEELLDYIRATATSVYHPAGTCRMGSDADAVVDSKLRVRGLEGLAVADTSIMPTI
                     VTAPTNAAAIMIGERAAPFLLTEA"
     gene            5104..5850
                     /locus_tag="VEJ00_08565"
     CDS             5104..5850
                     /locus_tag="VEJ00_08565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX01251.1"
                     /translation="MTDSIAGKVVVITGASSGLGAAAARMLAKEGAAVVLAARRRDRI
                     QRLADEIIAGGGESFAMETNVTAYEQVKALIDASVRKYGRVDVVINNAGIMPLAPIER
                     LKIDEWDSMIDVNLKGVLYGVAAALPYMKVQKSGHFVFVSSVAGHKVAPGMAVYAATK
                     HGVRALAEGVRQEVKQYNIRTTVISPGAVRTELLDGISEPDVAEAVRARVEAIALPAE
                     SFARAVVFALSQPDDVNVNEILFRPTRQEL"
BASE COUNT         1163 a         1798 c         1737 g         1164 t
ORIGIN      
        1 gcaactagcg cgagagctgg gccaaccggt agtgatagat aacaagggtg gtgccggtgg
       61 catcctcggc acgatggaag tcgttcgggc ggcgccggac ggatacacgc tgctgatggt
      121 aagtccgtct gtgaccgctg ctaacccggc gatcaatccg aacgccggct atgatccggt
      181 taccgacttg acggcaatca caatcgttgc cgccgggccc actatattgg ccgtgcgcgc
      241 cgggttcccg gccaaaaatt atcaggaatt catcgcggag gtgaaaaaaa atcctgaccg
      301 atatacatat gccactcctg gcgttggagg cattctgcat ctggaggtcg agtacttcaa
      361 gtcactcacg ggcacgtcca tcaggcatgt acccttccgt ggcgctggac ctgcactcgt
      421 cgcggtcacg agcggtcagg tcgacatgat ggaagacgca ctgccgtcgt cgcttccgtc
      481 gataaacgcc ggcaagctcg tccccctcgt agtgtccgct cctacgcgaa tgaaggaaat
      541 tcccaacgtc ccgacattcg cagaggtcgg actgccgcag ctgaatcata tgagccattg
      601 gggcattctc ggacccaagg gtcttccgca agaagtgatt gacaaggtca atgctgcggt
      661 tcgtaaagcg gtgaatgatc caactgttaa gaagcgcatc gaagacagtg gagggtttgt
      721 cgttgcgagt tcgccgcaac agttctccgc cgacatcaaa gacctttaca cgcagctcaa
      781 gaaggtggta gccgatcgca aactgacaac ggagtaggcc gaggctcctt ctggcgagta
      841 tcgggtcgat tcgcaagcgt tcggcggaac gccgttatag atcgcccctc ccgccgcgtt
      901 ccaggacaca gtaccacctt cgtctcgcta gggccggctg cccaccgcgc cggtcaattc
      961 aggatggtgc gggcacacgt cgggcgggac agcgatcggg ccgacacccc gagtatcagt
     1021 ggaacgatcg ccctccgtcg gctgcaagtg cagcaccggt gacgtaggac gattgggcgc
     1081 tagtgaggaa gaggattacc tgagcgatct ccgcagggtc ggccatccgc ttgagcgcgt
     1141 agttctgtgt attgcctcga aagccgtctg ccattggagt gtccaccatt ccggggcata
     1201 ccgtgttgac ccgtattttg ggcgcgagct cagcggccaa tgcgcgcccc aagttgacga
     1261 cccctccctt ggatgcagcg tacgcggtca gtcctggcgc atttggaagg agacccgccg
     1321 cggacgcgat gttgacgata gtcgctgctg attcctgctc catccagggc aggcagcttc
     1381 tgatcacgag ataggtcccg gtgagattta catccagggt ccgtctccat acggcaaccg
     1441 gcgtttctgc aacggagcca tggggcatga tgccggctgc gttgacgatt ccatcgatac
     1501 cgcccaagcg ggtgctcgcg ctttcggctg ctttcgagat ctgatcctcg ttggagatat
     1561 cgacctccaa tggatggcca cccgtttgcc gggtggtcag cgccaatccg gatgcgtctc
     1621 gatcgaagag ggcaagcgac gctccttctt gggcgaatag cagtgccgtg gtgcgaccaa
     1681 tcccggatgc cgctccggtg atgacaatgc gacggccgcg cagtcgattc gctggaggcg
     1741 gcagttcagg tgccggtgcg cccggaacat tattgctcat tgtcatgcct ccgctcagcg
     1801 ctgaacaaac gggttcgcgg gcgcgtgggc ccccgaggaa atcatcacgc tcttggtctc
     1861 gaaatactcc ttgatcgcct cgataccgct ctcgcgacca agtccagaat gcttcattcc
     1921 gccgatcggc accatgtagc tgtacgctcg gtacgtattg acccagacca taccggcgcg
     1981 cagtgccttc gacatccgga ccgctcggcc gatatcgcgc gtccagaccc ctgccgccaa
     2041 gccatagatc acgtcattgc caatgcggat ggcgtcttcc tccgtgtcga atccaatgac
     2101 ggaaagcacc gggccgaata cttcttcctg cgcgatccgc atccgattcg tgacatccgt
     2161 gaagatggtc ggctggacga acatcccgcc cttcaggccc tccccggacg ccggcgatcc
     2221 gcccaatacg cagcgagcgc cctctgcctt cgcgagctcg atgtagtgca gcactttctc
     2281 atactgcggc ttggtggaga tcggcccgat ctgggtgtcc gcgctgaacg gatctccaag
     2341 tttcaggttc ttggccatgg ccgccaggcg ctcggtgaat tgctgacgaa tcgagttctg
     2401 caccagtagc ctggaacccg cggtacacat ctgtccggtg gcgccaaaga tgcctgccac
     2461 ggccccgacg caagcaaggt ccaggtcggc atcctcaaat acgatgttcg gcgacttgcc
     2521 acccagctcc atcgtgaccc gtttcagtcc gcgtgcggct gcctcataga tcctcgcacc
     2581 agtcacatcg gatccggtaa acgtgatctt cgccacgtcc cggtgctcaa cgagcgccga
     2641 cccgatttcc tggccaaggc ccgttaccac gttgatgacg ccatccggga ggcctgcctc
     2701 cttggtcagt tcagcgaact ccagcgaact cactgaggcg aactcggacg gtttcaggac
     2761 taccgagcag ccggcggcaa gcgcaggggc acacttaagg gcaaagaact gtaacggcga
     2821 attccatgca gtgatcgcca ccaccacgcc aacaggctcg tgcagggtca ttgccaagac
     2881 gtccgccttg tccactggga tgacagtgcc ttcgatcttg tcggctaatc cggcgaagta
     2941 gtaccagcag tcgggaagcg acttgagctg gccgtgcatc tccgccagga tcttgccgtt
     3001 gtcccgcgat tcgatctcgg ccaagttctg ggcatgccgc gtttcgccta cgagatcgcc
     3061 gatgcgacgc aaaatcttgc ctctctcgga cgctgtcatc gtgcaccacg ggcctttgta
     3121 catggcatcg tgcgcagcag cgacggcgcg gttggcatct tccgccgtcc cgcgcgcaat
     3181 cttcgcccac gcaacgccct gatacgggtc gaacgtttct agcgattggc cgctgaccgg
     3241 cgcgacgtat tttccgttga tccagtgctg ataagtcttc attgcattac cctagggtcg
     3301 ttgatagaaa tgctggtgag ttggttacgc ctcggtgagc aaaaacggtg ctgcgcgttc
     3361 gccaatcatg atcgcagccg cgtttgtcgg cgccgtcacg atagtgggca tgattgaggt
     3421 atcggcgacc gccagtccct ccaggccgcg cacccgaagc ttcgagtcaa ccaccgcgtc
     3481 cgcgtccgac cccatcctgc atgtgccggc agggtggtag accgaggtgg cagtcgcgcg
     3541 gatatagtcg agaagctctt cgtcgctgtt cacgttcagg cccggacgct cttcgcgctc
     3601 gatctcgtcc cggagtggct tcgtcgctgc gacatgacgg gccgcacgca ggcccgctac
     3661 catggctcga cgatcgcttt cggcctcgag gtaattgggt tgaattaccg gtgggtcctt
     3721 tgcggcgggc gaacgcaccc gcacagtacc ccgactttcc ggacgcatca ggcaactggt
     3781 gatcgtgaaa ccgggaaact cgtcgagtcc tttgcgatag tccgcactgc tgaacggcat
     3841 gaggatcaac agggtatcgg agcgatccag tttcggatgc gagcgaacga aggcgccgca
     3901 ttgccccccg gcaaaggtaa gtggacctcg gcggaacagg gcgtatttca aacccatccc
     3961 caccaagcga agtggctggc gggtttgagt gttgagcgta gcgtgacgac gacttcgcaa
     4021 tgcgagccgc gcctgaacat ggtcctgcag gttggcgccg acccccggga gctcatgccg
     4081 taccgcgatg ccggcggcct gcaaccactc cccacgcccg attccggaac gctgcagaat
     4141 cgctggtgaa ttgagcgccc ccgcacacag aacgacggct cgccgcgccc gcgcaacaag
     4201 cgactcacct ccgcggccat accgaacttc cgtcacacgg ccgctctcaa tcatgagccg
     4261 ctcgacttca gcattggttt tcagacagac attggaccgg cgctcagcat cgcgaaggaa
     4321 tgccgctgcg gagctggcgc gccgcccatt acgcaccgtg acctgaaaaa atccggtgcc
     4381 ttcctgggat ggtccgttga agtcatcgtt ccggtcgaga cccccagcta ccccactctc
     4441 gacaaaagcc tggcccaagg ggctgaagtc aaccaggtcg gatacggaga gtgggccacc
     4501 ggaaccgtga taatggttcg cgccacgttg ctggtcttcc gatttgcgga aaaacggcag
     4561 aacgtcgtcg tatccccagc ccgaattgcc tgcccgtgcc caatcgtcga aatcgtcccg
     4621 atgccccctg acatagacct gaccattgat tgaactggag ccgccaagga ccttcccgca
     4681 tggggcgaag agcttgcgcg catgcaattg ggacgacgct tcggtcgaga agcaccagtt
     4741 caatgtttcg tcgaagaacg tcttgctaaa gccgatcggg atgtggatcc aagggttcga
     4801 gtcgcgtcct ccagcttcca ggacgagcac ggtgtggtgt cgactctcac ttaacctggc
     4861 cgcgacacca cagccggccg acccggcacc gatcacgata tagtcgaaat cttcgatcgt
     4921 catacccgtc gttgcgcgca actccccatt tcgcgcagtg aattatcgac gctccatttt
     4981 cctggggcca gtcgtgaatt ttgcatggtc ggtaagttgc cctgaccagc gtcagggcag
     5041 cttatgcagc gaaaagaatt accgtctgga cgcgttatct aacgacggcc cacacttgcc
     5101 ggcatgacgg attccatcgc aggtaaggtt gtggtgatta cgggtgcgag cagcgggctc
     5161 ggagccgctg ccgcgcgcat gcttgcgaag gaaggggcgg ccgtcgtact tgcggcccgc
     5221 cgacgcgacc ggattcagcg tcttgcggat gagattatcg ccggaggagg cgagtcattc
     5281 gccatggaaa caaacgtcac ggcttacgaa caagtaaaag cccttatcga tgcgtcggtc
     5341 cgaaaatatg gacgggtgga cgtcgtaatc aataacgccg ggatcatgcc gcttgcgccg
     5401 atcgagcggc tcaagatcga tgaatgggat tcaatgatcg acgtcaacct caagggtgtg
     5461 ctttatggcg tagccgctgc tctcccatat atgaaggtgc agaagtcggg acacttcgtg
     5521 ttcgtgtcct cggttgccgg acataaggtt gctccaggga tggccgtgta tgccgcaacc
     5581 aagcacgggg tacgggcgct tgcggaaggc gttcgacagg aagtgaagca gtacaacatc
     5641 cgcaccaccg tcatatcgcc gggcgccgtg cggacggaac tgctggatgg catttccgaa
     5701 ccggatgtgg cagaggcggt gcgcgcccgg gtggaagcca tcgcattgcc cgcagagtct
     5761 ttcgcgcgtg ccgtcgtgtt cgcgctcagt caacccgacg acgtcaatgt gaacgaaatt
     5821 ctgttccgcc cgacgcgcca ggagctgtga gtcgggacat ga
//
LOCUS       DASPUL010000355         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1982636, whole genome shotgun sequence.
ACCESSION   DASPUL010000355 DASPUL010000000
VERSION     DASPUL010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982636"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VEJ00_08570"
     CDS             complement(<1..462)
                     /locus_tag="VEJ00_08570"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXX01252.1"
                     /translation="MQRVNTPEILDSESCPPEEVAVSLRDLSRINRWFGGVATTRKMI
                     ERVSAATGKKHFSMLEVAAGFGEVPKVACEYLSCKGITLELTLLDRVRSHLLPGNRSV
                     VADALALPFPDGAFDLVSCSLFAHHLESAELARFADEALRVSGCAVLINDLI"
     gene            complement(539..2272)
                     /locus_tag="VEJ00_08575"
     CDS             complement(539..2272)
                     /locus_tag="VEJ00_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXX01253.1"
                     /translation="MLPKSLRPLLPYLKRYRWGYAAGTACVFLTNGILVLLPLVIGRA
                     FDDLLHGGHKLLLYAGLLLAIAVSKGIFQFLTRWIVIGISRDIEFDLRNDLFERLESL
                     SYSYYQRNRTGDIMARATNDLSAVRNLLGPAIMYTANTIVLTAGALAFMVSISPRLTF
                     YTFLPLPVASIVIQYVGRQIHDRFERIQAMFSDISARAQENFSGARLIRAYVQEEAEI
                     RAFEAANQEYIRRSLRLVRLMGMLWPTLELMLGCAMVMVLWLGGREVIAGHITPGGFM
                     SFLTYMTQLMWPVIALGYVINLFQRGTASLGRLNEILQEKPEIKDAPKAQSREIEGEI
                     EFRALNFSYDGKEVLHDVNLRVPAGSSLAIVGPTGSGKTTLVNLIPRIYDAEPGMVLI
                     DGRPIRELSLASLRRNIGFVPQETFLFSDRIRENIALGVDAATAEEIHRAAEAANIAA
                     DIESFPEGYETLVGERGITLSGGQKQRTAIARALIRNPKILILDDALSSVDTQTEDRI
                     LNHLRDVMQGRTTIFISHRVSTVRNADRIAVLHGGRIVELGTHDQLLALNGYYSDLYN
                     KQLLEEELAEV"
     gene            complement(2471..2647)
                     /locus_tag="VEJ00_08580"
     CDS             complement(2471..2647)
                     /locus_tag="VEJ00_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01254.1"
                     /translation="MKLSYLFDAGWLFFALWAAILVALNCAAFAGDLFPSRPLSGSNP
                     DHPRSTSASRRMAG"
     gene            complement(2741..3238)
                     /locus_tag="VEJ00_08585"
     CDS             complement(2741..3238)
                     /locus_tag="VEJ00_08585"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HXX01255.1"
                     /translation="MKSIHLRLLIATLAVLLGTTIGKSQTATSTPPPARVHTHQYGMH
                     RHGMGFFPRSLNLTDAQHAQIKAILEKERTVMKPLFQQSHQIDQQLRQFAEGTFDEGK
                     VRALAAQKAQVEVELAVQKTRIHNELFHLLTTDQQAKMKEIEANRQTRMQKHMQDAPA
                     APPEQ"
BASE COUNT          687 a         1075 c         1060 g          691 t
ORIGIN      
        1 aatcaggtcg ttgatcaaca cagcgcaccc gctcacccgc aacgcctcgt ctgcaaatcg
       61 cgcgagctcg gcagattcca ggtgatgggc gaatagactg cagctgacca gatcgaacgc
      121 cccatcgggg aagggaagcg cgagcgcatc ggccacgacc gagcggtttc ccgggagcag
      181 atgcgagcgc acccgatcca gcagcgtcag ttccagcgtg attcctttgc acgacaggta
      241 ctcgcacgca actttcggga cttcgccaaa tcccgcggcg acctcaagca tcgaaaagtg
      301 ctttttcccg gtggccgcag acacgcgctc gatcattttg cgcgtggtgg cgaccccgcc
      361 aaaccagcgg ttgatgcgcg acagatcgcg cagcgaaacc gcgacctcct ccggcggaca
      421 gctttccgaa tcgaggatct ccggggtgtt aacgcgctgc atggtgagtg aacacaacca
      481 ggtttgtcat tccgagcgca gcgaggaatc tgctttttgc cagacgtggc gcaccgcttc
      541 agacttccgc cagctcttct tccagcagct gcttgttata caggtcgctg tagtagccgt
      601 tcaaggccag gagttggtcg tgcgtgccca gttccacgat ccgcccgcca tgcagcacgg
      661 cgatgcggtc ggcgttgcgc acggtcgaga cgcgatgcga aatgaagatg gtcgtgcggc
      721 cctgcatcac gtcgcgcagg tggttcagaa tcctgtcttc cgtttgggta tcgacgctgg
      781 aaagcgcatc gtccagaatc agaattttcg gattgcggat cagcgcccgg gcaatcgccg
      841 tgcgctgctt ctggccgccc gagagcgtga ttccgcgctc tccaaccagc gtttcgtagc
      901 cctccggaaa gctctcgatg tcggctgcaa tgttcgccgc ctcggccgcc cggtgaattt
      961 cctccgccgt ggccgcatcc accccgaggg cgatgttctc gcgaatgcgg tcgctgaaca
     1021 ggaaggtctc ctgcggaacg aatccgatgt tgcgcctcaa cgacgcgagc gaaagctcgc
     1081 gaatcggtcg gccgtcgatc agcaccattc ctggttcggc gtcgtagatg cggggaatca
     1141 ggttcaccag agtcgtcttc cccgatcccg tgggcccaac aatcgccagg ctgctgcctg
     1201 ccggtacgcg cagattcacg tcgtgcagca cctcttttcc gtcgtaactg aaattgagtg
     1261 cccggaattc gatctcccct tcgatctcgc ggctctgtgc cttgggcgca tccttgattt
     1321 ccggcttttc ctgcaggatt tcattcaacc ggcccagcga tgccgtcccg cgttgaaaca
     1381 ggttgatcac gtagccgagg gcgattaccg gccacatgag ctgcgtcatg tatgtgagaa
     1441 acgacataaa accgccgggg gtaatatgcc ccgcgatcac ctcacgtccg ccaagccaca
     1501 gcaccatgac catggcgcat ccgagcatga gttccagcgt gggccagagc atgcccatga
     1561 gccgtaccag ccgcagactg cggcgaatgt actcctggtt cgccgcctcg aaggcgcgaa
     1621 tctcggcttc ttcctggacg taggcgcgga tcagccgcgc tccggaaaag ttctcctgcg
     1681 cccgcgccga aatgtcggag aacatcgcct ggatacgttc aaagcggtcg tggatctgcc
     1741 gcccgacgta ctggatcacg atgctcgcca ccggcagcgg gagaaacgta taaaacgtca
     1801 gccgcgggct gatcgacacc atgaacgcca gtgctcccgc cgtcagcacg atcgtgttgg
     1861 ccgtgtacat gatcgccggg cccagcaggt tgcgcacggc actcaggtca ttagtggcgc
     1921 gcgccatgat gtcgcccgtg cgattgcgtt ggtagtagga gtacgacagg ctttccagtc
     1981 gctcgaacag gtcgttgcgc aggtcgaact cgatgtcacg cgagatcccg atgacgatcc
     2041 accgcgtcag aaactggaaa atgcccttgc tcaccgcaat ggcgagcagc aggccggcgt
     2101 agagcagcag tttgtggccg ccgtgcagaa gatcgtcgaa agcccggcca atcacgagcg
     2161 gaagcagaac caggatcccg ttggtcagaa agacgcaagc cgttcctgcc gcgtagcccc
     2221 agcggtagcg tttcaggtaa ggcaggagcg ggcgcaggga cttgggcaac atgcaaattc
     2281 agtagatgaa attgtagcag gcagggtgca gcttttccgc gcggccccag gccaggagcc
     2341 tgaggccgcg cccgacgggg gcttcctctg agaacgggtt gcgccgccat tcaggcggcc
     2401 gcgacggcca tgccagatga aactgcggaa attcctttga ggcactgcgg acgtgaggac
     2461 gggccgattg tcagcctgcc atccgccggc ttgcggacgt tgacctgggg tggtccgggt
     2521 tcgaaccgga gagcgggcgc gagggaaaca gatcgccagc aaacgcggcg cagttcagcg
     2581 cgaccaggat ggcagcccat agggcgaaga agagccatcc ggcgtcgaac agataagaca
     2641 gtttcatcgc aacgctactc ccgctggcgc ggggccgcag atcagcccag ccccgcgccg
     2701 ttcaacctgg aaccctgcca cagagtccca cttccctcag ctactgctct ggcggcgcag
     2761 ctggcgcgtc ctgcatgtgt ttctgcatgc gggtttgccg attggcttcg atctccttca
     2821 tcttggcctg ctggtcggtg gtcagcagat gaaacagttc gttgtggatg cgcgtcttct
     2881 gcaccgccag ctccacttcg acctgcgcct tctgcgcagc caaagcgcgc actttgccct
     2941 cgtcgaaggt cccttccgca aactggcgga gctgctggtc tatctggtgg ctctgctgga
     3001 agagcggctt catcaccgtg cgttccttct ccaggatggc cttgatctgc gcatgttgcg
     3061 cgtcagtcag gttcagagag cgcggaaaga atcccatccc gtgcctgtgc atgccgtact
     3121 gatgggtgtg cacccgcgcc ggcggcggcg tgctggtcgc ggtttgtgac ttgccgattg
     3181 tggtaccaag caaaaccgct agcgtagcaa tgagcaagcg aaggtgaatc gatttcatgg
     3241 tgtgactccc ggcttcttcc cggaggtccc gggattgtgc gtgcttttat agcaacacgg
     3301 cggcaggcag aaaagccgta aaaaatccgt caagcagagt aaagttttgt caagacgttc
     3361 cactttcgac agcctccagc ggctggcagc ggtttgacaa ttctttacta cgaaccgttt
     3421 tgctcatggt gcacaatgat gttcgggaga caatggaacg aattctggta atcgacgatg
     3481 acgtcgagct ctgccacctg gtcgacgagt acc
//
LOCUS       DASPUL010000356         6161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1709233, whole genome shotgun sequence.
ACCESSION   DASPUL010000356 DASPUL010000000
VERSION     DASPUL010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1709233"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1566)
                     /locus_tag="VEJ00_08590"
     CDS             complement(<1..1566)
                     /locus_tag="VEJ00_08590"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXX01256.1"
                     /translation="MANHAVSQDVLVGQRLGHYRIAEKIGAGGMGEVYLAHDEHLERE
                     VAIKVLPPGTLVDEHARKRFHKEALTLSKLNHPNIATIHDFDTHQGVDFLVMEYIPGI
                     TLSEKLATRPLLEKEVVALGTQLADGLSAAHEHGVFHRDLKPGNLRLTGDGRLKILDF
                     GLAKLRLPVTASATTESLSETHAMAGTLPYMAPEQLLGGEIDARTDIHAAGSVLYEMA
                     TGKGPFAEVERSQLIGAILRKPPLPPTTLNPKLSPELERIIGKCLEKEPENRYQSAKE
                     LAIDLRRLQTGVLSATKFAARPAKWSYAKLAGLGLGSLAAVVVLLLAFNVGNWRQRLL
                     GRVQTPHIESLAVLPVKNFSGDPSQDFFADGMTDALIADLAQIKAIKVISRTSVMHYK
                     GTSDTLPQIAGELGVDGIVEASVVRSGDRVRLTAQLIDARQDRHLWANNYERNMTDVL
                     GLQSELVQAITGEIRVQLAPQESERLKTTRHVDPDVYDTTLKGKETLEYATREVQIRQ
                     AIELFQKAIDRDPT"
     gene            1931..2305
                     /locus_tag="VEJ00_08595"
     CDS             1931..2305
                     /locus_tag="VEJ00_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01257.1"
                     /translation="MNTVSLTGGLLSISNTLTRGSRIELLFLTQAGPVLGSAEMLPAI
                     TAMQQPFRFVSLEEAAQRRLRSVIQSYIQPAPEAWIQKYRAALDQTPRRRSSFAVVLA
                     ALAAGVLCLGSAAWLLHTFPLK"
     gene            complement(2369..4267)
                     /locus_tag="VEJ00_08600"
     CDS             complement(2369..4267)
                     /locus_tag="VEJ00_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HXX01258.1"
                     /translation="MSATAQAAPQATSLPSETPDDFVPATDSFGYIKRTVMIPMRDGV
                     KLYTVILIPKGAKNAPILLTRTPYSAKTQTSHAASSHLGPILNGYDNAVEVIVDGGYI
                     RVVQDVRGKYDSEGDYVMTRPLHGPLNPTPVDHSTDTYDTIDWLVKNVPESNGRVGIL
                     GISYDGFLPLMALVNPHPALKVSVPMNPMVDGWMGDDWFHNGAFRQQNLPYIYEQEAN
                     RKNEEKWWTGHFDDYDVYMDAGSAGELGRRHGLEQVGFWRKLLEHPSYDSWWQEQAMD
                     RILAAQPLKVPMMLVDSLWDQEDIYGAPAVYKALKPKDTNNDKVFLVLGPWHHAQEIG
                     DCSNLGALRFHTDTGLYFRQNVLAPFLAQYLKDGAPKADVATVTAYETGTNEWRRLPA
                     WPAGCASGCPVRATRLYLEAGLKLGFKAPKAGDTPYEEYVSDPAKPVPFRSRPIQPVA
                     YGDGYTWSEWLVDDQREPSGRPDVVVFTSDVLKEPVKISGQPVANLIASTSGTDSDWV
                     VKLIDVYPDEVAAEPWMGGYELMISADIFRGRYRESLENPKPIDSGKPLLYRWTLPTA
                     NHVFLPGHRMMVQIQSSWFPLYDRNPQTFVENIFWARPGDYKRATQRVFHSPDQASFI
                     ELPLVEMK"
     gene            4556..4930
                     /locus_tag="VEJ00_08605"
     CDS             4556..4930
                     /locus_tag="VEJ00_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01259.1"
                     /translation="MSKITQSLAILAVGGLMYVGSAAAQTTNTSGAGAGQVDPGHPRV
                     NQVNRRETRQQNRIANGVKSGKLTPGQTAHLEKGEQRLQNNEKKDMAKDNGHLTKADQ
                     KQLNKEANHMSKRIYDDKHPNK"
     gene            5142..5918
                     /locus_tag="VEJ00_08610"
     CDS             5142..5918
                     /locus_tag="VEJ00_08610"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HXX01260.1"
                     /translation="MAVDTQKIKPGLEIASLTDVGLQRSNNEDSCLYWEPVSDDDFHR
                     KGRLAVVADGMGGYEGGQEASRLAVETVRHVYDNQFNGDPQETLLLALAAAHETIQRY
                     GDEHLQFQGMGTTCTAISIVGHQLFFAHVGDSRLYLVRDTSVTRLTRDHSYVGRLVES
                     GIVRSEDAESHPQRHILTAALGSGREIVPDAPEAPIQLEEGDTLVLCTDGLWSLVGEA
                     ELRRTVWSNTPSQACTALVNLALERGGPDNITVLVLRVSS"
BASE COUNT         1203 a         1885 c         1799 g         1274 t
ORIGIN      
        1 ggtcggatct cggtcaatcg ccttctggaa cagttcgatt gcctgtcgga tttgtacctc
       61 acgcgtcgcg tattcaagcg tctccttgcc cttaagggtc gtgtcgtaaa cgtcaggatc
      121 gacatggcgg gtcgtcttca gccgctcgct ctcctgcgga gcgagttgca ccctgatctc
      181 ccccgtgatt gcctgcacta actccgactg caaccccaac acgtccgtca tgttgcgctc
      241 atagttgttc gcccaaagat gccggtcctg ccgagcgtcg atgagctgag cggtaagccg
      301 cacccggtcc ccggagcgca ccaccgaggc ctcgacgatc ccgtcgaccc ccagttctcc
      361 ggcgatctgc ggtagggtgt cgctcgtgcc cttgtagtgc atcaccgaag tgcgcgagat
      421 gaccttgatc gctttgatct gggcgagatc ggcgattaac gcatcggtca tgccatcggc
      481 gaagaagtct tggcttggat caccggagaa gttctttact gggagcactg ccaacgattc
      541 gatgtgtggc gtttgaactc gaccgagcaa gcgttggcgc cagttgccta cgttgaaagc
      601 gagcagcagc acgacaaccg ccgccagact gcctaagcct agtccagcta acttcgcata
      661 cgaccacttg gccggccttg ctgcaaactt ggttgcactc agcacacctg tctgcagccg
      721 ccgcagatca atggccagtt ccttcgccga ctggtagcga ttctcgggtt ccttctcaag
      781 acacttcccg atgatgcgtt ccaattctgg agacagctta ggattgagag ttgtgggtgg
      841 tagcggcggc ttgcgcagaa tggcaccgat cagttgcgag cgttccacct cggcaaaagg
      901 gcccttcccc gtggccatct cgtacagcac cgacccggcc gcatgaatgt cggtacgagc
      961 gtcgatctct cctcccagca actgctccgg cgccatgtag ggcagcgttc ccgccatggc
     1021 gtgcgtctcg ctcagactct ccgttgtcgc gctcgcggtc actgggagcc ggagcttcgc
     1081 cagcccgaag tccagaatct tcagcctccc gtcgccagtc agtcgcaagt tgccgggctt
     1141 caggtcgcga tgaaagaccc cgtgttcgtg agctgcggac aggccatcgg cgagctgcgt
     1201 ccctagtgcg acgacttcct tttcgagtag aggccgggtg gccagcttct cgctgagggt
     1261 gattcccggg atgtactcca tcaccaggaa gtccacaccc tgatgcgtgt caaagtcatg
     1321 aatcgtagca atgttgggat ggttcagctt ggagagggtc agggcttcct tatggaagcg
     1381 cttgcgggca tgctcgtcga ctaaagttcc aggcggcagg accttgatcg ctacttcgcg
     1441 ttcgaggtgc tcatcgtggg cgaggtagac ttcccccatg cccccggcac cgatcttttc
     1501 ggcgatgcgg tagtggccga gcctctggcc aacaagcacg tcctggctga ccgcgtggtt
     1561 ggccatgcgg aaagtctcct tcccgcgccc tatgagacaa atcgtaggct ttaccacgag
     1621 gcgagtcaac gcagaacaac aattcttagc gaatacctga actagacagg gaggcgggct
     1681 gaagggctga gcgattccta gcgcagttct cactcttttc agatccgcca ggcgcccgcg
     1741 agggtctgtg ggtcgaggtg ctgctccttt tgtaatcttc tatatatgta cgcgcacgaa
     1801 tcatactcgg tcttaagcca tgaactatgt tgcgcaatcg cacccggcct ggcgctgccc
     1861 gcgggtacag cttgctgata tcacacctgc cgtgctgcga ttgcccgatg gaagcgggtc
     1921 ccgagccata ttgaataccg tttcgctcac cggcggcttg ctcagcattt cgaacacgct
     1981 gacccgcggc tcccgaatcg agctgctctt cctgactcag gccggtccag tgctgggctc
     2041 ggcggaaatg cttccggcca ttaccgcgat gcagcagccg ttccgctttg tttcccttga
     2101 ggaagccgct cagcgcaggc tgcgatccgt gatccagtct tatatccagc ctgccccgga
     2161 agcctggatc cagaaatatc gggcagcgct ggatcagact cctcgacggc gaagctcctt
     2221 tgcagtggtt ctggccgccc tggccgctgg ggttctttgc ctcggaagcg ccgcctggct
     2281 gctccacaca tttcctctga aatagaggca aacacgcccg gcgaccatcc gtcacgctgg
     2341 gttcgaagcc caattccttc gtccagcttc atttcatctc aaccagcggc agctcgataa
     2401 agctcgcctg atcgggcgag tgaaatacgc gctgagtcgc cctcttgtag tcgccgggcc
     2461 tagcccagaa gatgttctcg acaaaggtct gcgggttgcg atcatagagc gggaaccagc
     2521 tcgactggat ctgcaccatc atgcggtgtc cgggcaagaa aacgtggttc gccgttggca
     2581 aggtccagcg atagagcagc ggcttgcccg agtcgatggg cttcgggttc tccaagcttt
     2641 cgcgatagcg accacggaag atgtccgcgg agatcatcag ttcgtagcct cccatccacg
     2701 gctcggcggc gacttcgtcc ggatagacgt caatcagctt cactacccaa tcggaatcag
     2761 ttccgctcgt cgaggcgatg aggttcgcga ccggttgacc gctgatcttt accggctcct
     2821 tcagcacgtc cgatgtgaaa actacgacgt ccggccgccc cgagggctcg cgctgatcgt
     2881 cgaccagcca ctccgaccac gtgtatccat cgccatacgc aaccggctgg atcgggcgcg
     2941 agcgaaacgg cacgggcttg gccgggtcgg agacatattc ttcataggga gtgtcccccg
     3001 ctttgggcgc cttgaagcca agcttcaggc ctgcttccag ataaagccgc gtggcacgga
     3061 ccgggcagcc gctggcacat cccgcgggcc acgccggaag acgccgccat tcattcgtcc
     3121 ccgtttcgta ggctgtaacg gtggcaacat ctgccttggg ggcgccgtct ttcagatact
     3181 gcgcaagaaa gggcgccaga acgttctggc ggaaatagag ccccgtgtca gtgtggaagc
     3241 gcaatgcgcc cagattgctg caatcgccaa tctcctgcgc gtgatgccac ggtccgagca
     3301 cgagaaagac cttgtcattg ttagtgtcct tcggcttgag cgccttgtag accgcgggcg
     3361 cgccgtagat gtcttcctgg tcccacaggc tgtcgacgag catcatcggt accttcaacg
     3421 gttgcgccgc cagaatccgg tccatggcct gctcctgcca ccaggagtcg tagctcggat
     3481 gctccaagag cttgcgccag aatcccacct gttccagccc gtgacggcgt cccaactccc
     3541 ccgctgaccc ggcatccatg tagacgtcgt agtcgtcgaa gtggccggtc caccactttt
     3601 cttcattctt gcggttggct tcctgctcgt agatataggg caggttctgc tggcggaatg
     3661 cgccgttgtg aaaccagtcg tcgcccatcc agccgtcgac catgggattc atcggcactg
     3721 agaccttgag cgcgggatgc ggattcacca gcgccatcag cggcaggaaa ccgtcgtagg
     3781 aaatcccgag gatgccgact ctgccgttgc tctcggggac gttcttcacc agccagtcga
     3841 tcgtgtcgta ggtgtcggtg gaatgatcaa ccggggttgg gttcagcggt ccgtgcagtg
     3901 ggcgggtcat gacgtaatcg ccttcggagt cgtatttgcc gcggacatcc tgaaccaccc
     3961 ggatgtatcc gccgtcgaca atcacctcca ccgcgttgtc gtagccgttc aggatgggtc
     4021 ccagatgaga acttgcggca tggctggtct gcgtcttggc gctgtagggc gtgcgcgtca
     4081 gcaagatagg agcgtttttc gcgcctttgg ggatcagaat cacggtgtac agcttcacgc
     4141 cgtcgcgcat tggaatcatg accgtgcgct tgatgtagcc gaaactgtcg gttgccggca
     4201 cgaaatcgtc cggggtttca ctcggcagtg aggttgcctg gggcgcggct tgcgctgttg
     4261 cggacaaggg agattgcagg caaaaagatg ccaaagccag gagcaaaaag aaggcaacag
     4321 aggtatcgac gcggcatttc gtcatggtca gggttcctag gatgcggcga ggaatagtaa
     4381 accgtggccg cgcgagttgc caaatgcaca cgattcggtt ctacttgcag gcgtgcggat
     4441 tgccgagccc gtcgggtgaa gatgccgcat ttgttcagca tcacgcacta caccttcgta
     4501 accagccgca agaaacaaaa caaaactaga cttctgggtg ggggttcact ctcgcatgtc
     4561 caaaatcacg cagtcgctcg caatccttgc agtgggtggt ctgatgtatg tcggatcggc
     4621 cgctgctcaa accaccaaca cgtccggcgc cggcgcaggc caggtcgatc ccggacatcc
     4681 gcgtgtcaac caggtcaacc gccgcgagac gcgccagcag aaccgcattg ccaacggggt
     4741 gaagagcgga aagctgaccc cggggcagac cgcccatctg gaaaagggcg agcagcgcct
     4801 gcagaacaac gaaaagaaag acatggccaa ggataacggt cacctgacca aggccgacca
     4861 gaagcagctc aacaaggaag ccaatcacat gagcaagcgc atttacgacg acaagcatcc
     4921 gaacaagtaa gccttccgtc gtctcccgtg ttttgacagt gccggtagaa gcgagagccc
     4981 gcggactgcg ggctttttct ttctctgcgc acaggccgtt cggccgctga acgcccaacg
     5041 ctgcaacggc gaaacccgat gccttccctt ataatggcgc gagatctggt ctcctcgttt
     5101 gcacccaggc cgttaacggg gtagactcgc tcgcctcagc tatggcggta gatacgcaaa
     5161 agatcaagcc cggactcgaa atcgccagcc tgaccgacgt cggtctgcag cgttcgaaca
     5221 atgaagactc ctgcctgtac tgggagccgg tttcggacga cgacttccac cgcaaaggcc
     5281 ggctcgccgt ggtggccgat ggcatgggtg gatacgaagg cgggcaagaa gccagccgtc
     5341 tggcggtcga gaccgtccgc cacgtttatg acaaccagtt caacggcgac ccgcaggaaa
     5401 ccctgctgct ggccctcgct gccgcccacg aaaccatcca gcgctatggc gatgagcatc
     5461 tgcagtttca aggaatgggg acgacctgca ccgccatctc catcgtcggt caccagcttt
     5521 tttttgccca tgttggtgac agtcgcctgt acctcgttcg cgacacctcg gttacccgac
     5581 tcacgcgcga tcattcctac gtcggacggt tggtcgagag cggcatcgtg cggtccgagg
     5641 atgccgagtc gcatccgcag cgtcacattc ttaccgcggc cctgggctcg ggaagggaaa
     5701 tcgtgcctga cgcccccgag gctcccatcc agttagagga gggggatacg ctggtgcttt
     5761 gcaccgatgg cctgtggagc ctggtcggcg aagcggaact gcgccgtacg gtgtggtcca
     5821 acaccccctc gcaagcctgc accgccttgg tgaatctggc ccttgagcgc ggcggtcccg
     5881 acaatatcac cgtgcttgtg ttgcgcgtct cgagctgact caaggcacgg cgcccgcgga
     5941 tctcgccctg gggcagtccc agatgtggga caccgtctcc ctcacgaacc gttcgttcca
     6001 acaaactgca atcccctgca catcgcgttc taaccatgtg taaggcggtc gctaagctgc
     6061 tcatgctaaa ggactgaatt cctgcgcagc atctgaaggt gtggaatccg gcgtgcaatg
     6121 tcgttaacgt caataaagtc tattattaca atagacataa t
//
LOCUS       DASPUL010000357         4665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1018479, whole genome shotgun sequence.
ACCESSION   DASPUL010000357 DASPUL010000000
VERSION     DASPUL010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018479"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..698)
                     /locus_tag="VEJ00_08615"
     CDS             complement(117..698)
                     /locus_tag="VEJ00_08615"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXX01261.1"
                     /translation="MITASELSQGMVIRVEGHVYRVLAVEAKAAAAKLGGVVKAELSN
                     VHTGRLWEARFRPQERLEDLPVDRRNLEFLFHADNACTFMDPNSFEQVEVSDEIVGPA
                     ASFLQSGANVPVEFFEGKPISALLPDIVEARVVDTAPPSHSQQDSAWKEARLENGASI
                     RVPLFIAPNEHVRVDLRTGRYIERVHNDRKRVA"
     gene            complement(1167..2294)
                     /locus_tag="VEJ00_08620"
     CDS             complement(1167..2294)
                     /locus_tag="VEJ00_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HXX01262.1"
                     /translation="MPLESLEFHTSLQKLLIALILIFVPLTVFGFYVALQGDNQIRQA
                     SGENFRSLTSTVAESTSEYVAARVREVSVVANTAGPVDAVTASNRQYEHLSDEAVRAK
                     IGAIEQAWVTSQTDGVSEKMLTSDLARYLRRMRELSPTLLKITIADVTGATVAATDRP
                     AHYFQTDREFWQSLYSQGHGAIHVSDLRYDDETHLYYVSIAYPILQEGTGRFIGAVTA
                     MADVSPLFAQLNRRQIGRTGRLFLVRDDGFVVEAPGVTPALKIKSEEYAAIHDALGSL
                     RGRSAGYIFTTLPNHETYLIGFADTGLNEAYPNLPWIVVASQESREVTGPVRSTIAFA
                     LLVMILALLMLSLLAAYVFLHRRQQLEDIETPEDRPRPMAA"
     gene            complement(2721..4217)
                     /locus_tag="VEJ00_08625"
     CDS             complement(2721..4217)
                     /locus_tag="VEJ00_08625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXX01263.1"
                     /translation="MAAAGLLWLAHAAVVVTLGTNPPGPMWSDLIQFALGGVLICAIL
                     DAARRSEGLARSFWRLAAFAYMAWMVAQGLGLFNDFAESAGISWITNLLFCFWFAPLA
                     MAVFLDPDNNAGYLDTLIILDFIQAVLVCVAAYIYFFYLPKSEDPSQLAHSVWAPYFV
                     GYGSVVGAFLLRAAVTRSRDVRALFGRFGVFLGLSGCADALFYYGPGRGLHTGAWFDL
                     LWSALLVGPTVIAATWKQAESAELSSEPARREKHIYSEIFYLFYPLLVLFMSLRIARE
                     RLGLAAAVVFLSFVCSSARQLVTQQRLLVAKDALRREASRDSLTSLWNRKAILDILER
                     ELLRAERDQQPVGLIMVDVDHFKNVNDTRGHAAGDAVLRIIASGIAAVVRPYDSVGRC
                     GGEEFLIVAPGCGPAETWELAERVRTHVAGCSIMVGGAGVKVTLSLGIATSQSSADTE
                     QLLHAADTALYQAKNAGRNRVEPCIAQKVGRAAAAQGSSTTPDRDFWL"
     gene            complement(4453..>4665)
                     /locus_tag="VEJ00_08630"
     CDS             complement(4453..>4665)
                     /locus_tag="VEJ00_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01264.1"
                     /translation="RSRLVAISSDLKDARRMRIQPTAKGTRLLHKGRQRRIEYLASHL
                     DTLSPDEAATLGVAIDILKRLLETWR"
BASE COUNT          895 a         1314 c         1657 g          799 t
ORIGIN      
        1 tagagatcct cgacggtttt gatgaagtgc gtctggccga cgatgatgtt ggcgtcggcg
       61 ggaaactcca gggcaacggc cttgatctcg agcatggctg gctccgtggc ggatggtcag
      121 gcgacccgct tgcgatcatt atgcacccgc tcgatgtagc gaccagtgcg gagatcgacg
      181 cggacgtgct cgttgggggc gatgaagagg ggaacgcgaa tcgaggcgcc gttttcgagc
      241 cgggcctctt tccaggcgct gtcctgctgg gaatgcgagg gaggcgcggt atcgacgacg
      301 cgcgcctcga caatgtcggg caggagggcg ctgataggtt tgccttcgaa gaactcgacc
      361 ggcacgttcg cgccggattg cagaaaactc gcggccgggc cgacgatttc atcggagact
      421 tcgacctgct cgaaagagtt ggggtccatg aaggtgcagg cgttgtcggc atggaacagg
      481 aattccaggt tgcggcggtc gacgggcaag tcctcgaggc gctcctgggg gcggaagcgg
      541 gcttcccaga ggcgtccggt gtggacgttg ctcagttcgg ccttgactac gcctccgagt
      601 ttcgcggcgg cggccttggc ctccacggca aggacgcggt agacgtggcc ttcgacgcgg
      661 atgaccattc cctgggatag ctcggaagcg gtgatcatac agttctcctg tcgagttgca
      721 tcagtgcccg gtcggttcgc gctcgcttgc aaaggcgacc acaggggcta aagccccaat
      781 ctgtgcagac cctttgacgc ggccctaaag ggccgctctt ccacgtgcta gacgcgccac
      841 ttgcggcccc tcggcgcggc ttgctttccc ccgcaaaggg cgaccacaga gggctaaagc
      901 cccgaaaatt gcgaacgcgt gcgcggccct gaagggccgc tcttccacgt ggcacgagag
      961 gccagttgcc gctccttcga ccttcgcgga ttgttgcaac cgcaagagcc gctcttccgc
     1021 gtgcgccgag aagccggttg cagccccttg gcaggcttcg cttggctgag ggcaggccct
     1081 gaagggccgc tcttaaacgt catcggaaac aacaactagg ttcccgtcgc gctgcgcccg
     1141 gcccagcttc ggcaagcctc gcaacatcaa gcggccatgg ggcgcggccg gtcctccggg
     1201 gtttcgatat cctccaactg ctgccggcga tgcaggaaca cgtaggctgc gagcagcgac
     1261 agcattaaca gagccaggat catcaccagc agggcgaatg cgatggtgct gcgaaccggg
     1321 ccggtgacct cgcgcgactc ctggctggct acaacgatcc acggcagatt ggggtaagcc
     1381 tcattcaaac cagtgtcggc gaagccaatc agataggtct catgattcgg cagcgtcgtg
     1441 aaaatgtaac cggcggagcg gccgcgcagg ctgcccagcg cgtcatggat ggcggcgtac
     1501 tcctcggact tgatcttgag agcgggagtg acgccggggg cttcgaccac gaatccgtcg
     1561 tcacgcacca ggaacaagcg gcccgtgcgt ccgatctggc ggcggttgag ttgagcgaac
     1621 agcggggaga cgtcggccat ggcggtgacg gcgccgatga aacggccggt gccttcctgc
     1681 aggatgggat aggcgatgct gacgtagtag agatgcgttt cgtcgtcata gcgcagatcg
     1741 gagacgtgga tggcgccgtg tccctgggag tagagcgact gccagaattc ccggtcggtc
     1801 tggaaatagt gcgccgggcg atcggtggcg gcgacggtgg ctccggtgac atcagcgatg
     1861 gtgatcttga gcagcgtggg actgagctcc cgcatgcggc gcaagtagcg agccaggtcg
     1921 gaggtgagca ttttctccga tacgccgtca gtttgggaag tcacccaggc ctgctcaatg
     1981 gctccgatct tggcgcggac ggcctcgtcg gaaaggtgct cgtactggcg gttcgacgcg
     2041 gtcacggcgt cgaccgggcc cgcggtgttg gcgacgacac tgacctcgcg cacgcgggcg
     2101 gcaacgtact ccgaggtgga ctcggccacg gtggaagtga gtgagcgaaa gttctcgccg
     2161 ctggcctggc gtatctggtt gtctccctgc agggcgacgt agaagccgaa aaccgtcagg
     2221 gggacgaaga tcagaatcag cgcgatcagc agcttttgca gtgaagtgtg aaattcaagg
     2281 ctttcgaggg gcatggggat tacctcctct tcttctgccg tcacgaggaa caacggcagc
     2341 gtcggatccg aaggggatcc gcaggttaag agaccgaaac gaccacacaa ttaggctatg
     2401 tgccgggagt ctcccccgtc tgtgattagc cgcacagggg tgggtgcgcg atgcgtctga
     2461 gggaaacgac cacctgccct ccggccctaa agggccgaac gttttggccg tcttacgcgg
     2521 ccctgaaggg ccgctcttcc acgtggccta aagcgccgga gatgacgctg aacggccgcg
     2581 gttcccccgt ggtcttaagc gccggaggtg accctgacgg accactcttc cgtggtacta
     2641 agtccggatg ttgttcttca gactttgctc cgggtgggcc taactgcttc aagcggcgaa
     2701 gggtggaatt ggaatcgcag ctacagccag aagtcgcggt cgggagtggt ggaggaaccc
     2761 tgggcggcgg cggcgcggcc gactttctgg gcgatgcaag gttcgacgcg gttgcggccg
     2821 gcattcttgg cctgatagag ggcggtgtcg gcggcgtgca ggagttgttc ggtgtcggcg
     2881 gaagattggc tggtcgcgat gccaaggctg agggtgactt tgacgccggc gcctccgacc
     2941 atgatgctgc atccggcgac gtgagtgcgg acgcgctcgg ccagttccca ggtttcggcg
     3001 gggccgcagc cgggggcgac gatgaggaat tcctcgccgc cgcagcgtcc aacggaatcg
     3061 taggggcgga ccacggcggc gatgccgctg gcgatgatgc gcaggaccgc gtctccggcg
     3121 gcgtggccgc gggtgtcatt gacgtttttg aagtggtcga cgtcgaccat gatgaggccg
     3181 accggctgct gatcgcgttc ggcgcgcagc agctcgcgct cgagaatatc gaggatggcc
     3241 ttgcggttcc acaggctggt cagagagtcc cgggaggcct cgcggcgaag agcgtccttg
     3301 gctacgagaa gacgctgctg ggtgacgagc tggcgcgcgc tggagcagac gaaggacagg
     3361 aagacgacgg cggcggccag tcccagacgc tcgcgggcga tgcgcagcga catgaacaga
     3421 accagcagcg ggtagaacag atagaagatc tcggagtaga tgtgtttttc gcggcgtgcg
     3481 ggctcggacg aaagttcggc ggactcagcc tgcttccaag tggcggcgat cacggtgggt
     3541 ccgaccagca gcgcgctcca cagaagatcg aaccaggcgc cggtgtggag accgcgtccg
     3601 gggccgtaat agaagagggc gtcggcgcat ccggagaggc cgagaaagac gccgaagcgt
     3661 ccgaaaagag ctcgcacatc gcgcgagcgg gtgaccgccg cgcgaaggag gaaggcgccg
     3721 accaccgatc catagccgac gaaatagggg gcccagacgg agtgggcgag ctgcgagggg
     3781 tcctctgact tgggaaggta gaagaaatag atgtaagccg cgacacagac cagcacggcc
     3841 tgaatgaaat cgaggatgat cagggtgtcg agatatccgg cgttgttgtc gggatcgagg
     3901 aacaccgcca tggcgagcgg ggcgaaccag aagcagaaca ggaggttggt gatccaggag
     3961 atgccggccg actcggcgaa gtcgttgaag aggccgagac cctgagcaac catccaggcc
     4021 atgtaggcga aagcggccaa gcgccagaag gagcgggcca gaccctcgga tcggcgggcg
     4081 gcgtcgagga tagcgcagat caatacccca cccagcgcga actggatgag gtcggaccac
     4141 atcggcccgg gcgggttggt gcccagagtg acgactactg cggcgtgcgc cagccacagc
     4201 aacccggccg cggccacaag aatacgagaa cgcgccaaaa tacactttcc tttcgacggc
     4261 gatcgtgccg atagcaatcg acggatgaca gcgcaggggg gaattggccg gatgccggcg
     4321 tcccgaatac acccggaccc cacgcatcgt taatgccgga catacctcaa gtgcgacttt
     4381 ccagtatgga gggcggggaa gtgactttca tgtaaccaag gttaccttgg gagaattacc
     4441 gcagccggga gcttagcgcc aggtttcgag caaccttttc aggatgtcga tggctacgcc
     4501 gagggtggcg gcctcgtcgg ggctgagggt gtcgaggtgg gaggcgaggt attcgatgcg
     4561 ccgctggcgt cctttgtgga gcaggcgggt gcctttcgcc gtgggctgga tgcgcatgcg
     4621 gcgggcgtct ttgagatcgc tggagatggc gaccagacgg ctgcg
//
LOCUS       DASPUL010000358         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_472646, whole genome shotgun sequence.
ACCESSION   DASPUL010000358 DASPUL010000000
VERSION     DASPUL010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472646"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VEJ00_08635"
     CDS             <1..376
                     /locus_tag="VEJ00_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXX01265.1"
                     /translation="LAALIVFIGFNLTFFPQFILGYLGMPRRYYDYSHMPEFQVLNVL
                     STAGATILGVGYLLPMIYFLWSMRYGKPAPANPWNAAGLEWQTPSPPPTFNFDEEPVV
                     TWEAYNYEELDAPHKEEVPVAG"
     gene            366..1073
                     /locus_tag="VEJ00_08640"
     CDS             366..1073
                     /locus_tag="VEJ00_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HXX01266.1"
                     /translation="MPDSSTAAVHAETHNPALLHHFATEEQQRDASSFGMWIFLATEV
                     MFFGGLFCAYLIYRLKYFGDFGAASKSIDALLGGTNTAVLICSSLTVVLAIWAAQRSR
                     RALMIAMLVLTMLFGVAFLGIKAYEYNDKFKEHHVPGASFSFEHVQIPGHPDQYANPQ
                     HAEIFFALYFIMTGLHALHMVVGLGIFTWLLWMAWKGRFTPEYHTPLEIGGLYWHFVD
                     IIWIYLFPLLYLIDRHP"
     gene            1098..1406
                     /locus_tag="VEJ00_08645"
     CDS             1098..1406
                     /locus_tag="VEJ00_08645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HXX01267.1"
                     /translation="MTEDSTSSHSKASSLTTYFSVWIALLVGTFLTYEAAKVDLGVFN
                     AAVALTIATTKALLVALFFMHLKGASEKLLKLVVICTIFFLIILLALSMADYGTRSWS
                     "
     gene            1689..2174
                     /locus_tag="VEJ00_08650"
     CDS             1689..2174
                     /locus_tag="VEJ00_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01268.1"
                     /translation="MKIVLIVVAVIAGLGILGVAGFGYFVYHVARTAHVRQEGNNVKL
                     ETPFGSVETSQDPQKAAQDLGVDIYPGAEVQKNGASDATIGKLHTVTAIFTSSDSLDK
                     VCSFYKSKFPNAMVATSDQNHCNIVSNDRKNTITINIQASGDTTKIQITNINQNAASS
                     N"
     gene            2262..>3247
                     /locus_tag="VEJ00_08655"
     CDS             2262..>3247
                     /locus_tag="VEJ00_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXX01269.1"
                     /translation="MRARIRGDVRFDPGSRALYATDGSNYRQVPIGVVLPRDNDDVLA
                     TISLCREFGAPLLCRGGGTSLAGQCCNVAVVLDFSRYMSRILEIDPARRIARVQPGVV
                     LDQLRNAAEKHHLTFAPDPATHDRCTIGGMIGNNSCGVHSVMAGKTDDNIEALDVVTY
                     DGTRLRVGENLIETRRDSRPRLSGGPELSGSADRAAQISSPLKQIAREYANLIRQKFP
                     NIPRRVSGYNLNYLLPENNFHVARALVGSEGTCVTILEATCRLVESPPERVLLVLAYP
                     DIYQCADHVPEIMAHRPIGLEGFDDLLVGYTRAKGINSEGLALLPEGNGWLMV"
BASE COUNT          652 a         1086 c          842 g          667 t
ORIGIN      
        1 gctcgccgca ttgatcgtat tcatcggatt caatctgact ttctttccgc agttcattct
       61 tggctacctc ggaatgccgc gccgttacta cgattacagc cacatgcccg aatttcaggt
      121 actgaacgtg ctttccaccg cgggcgcgac catcctcggc gttggctatc tgctccccat
      181 gatttatttc ctgtggtcga tgcgctatgg gaaaccggca cccgccaatc cctggaacgc
      241 cgccgggctc gaatggcaga cgccttcgcc gccgcccacg ttcaattttg acgaagaacc
      301 ggtcgtgacc tgggaggcat acaactacga ggagttggac gcgcctcaca aagaggaggt
      361 cccagttgcc ggatagttcc accgccgccg ttcacgccga aactcacaat cctgccttgc
      421 tgcaccactt cgccaccgaa gagcagcagc gcgacgcctc cagcttcggc atgtggatct
      481 tcctcgccac ggaagtcatg ttcttcggcg gcctgttctg cgcttacctg atctaccggc
      541 tgaagtactt cggcgatttc ggcgcggcca gcaagtcgat cgacgctctt ctcggcggca
      601 ccaacacagc ggtactcatc tgcagcagtt tgaccgtggt gctagccatc tgggctgcac
      661 agagatcccg ccgagcgctc atgatcgcca tgctcgtgct caccatgctt ttcggtgtgg
      721 cgtttctggg aatcaaagcc tacgaataca acgacaaatt caaagagcac cacgttcccg
      781 gcgcatcctt cagcttcgag cacgtgcaga ttcccgggca ccccgatcag tatgccaacc
      841 cgcagcatgc cgagattttt ttcgcgctgt atttcatcat gaccggcctg cacgccctgc
      901 acatggtggt cggcctcggc atctttacct ggctcttgtg gatggcctgg aaagggcggt
      961 tcacgcccga atatcacacg ccgctcgaaa tcgggggctt gtattggcac ttcgtcgaca
     1021 tcatctggat ttacctgttt ccattactgt atttgatcga tcggcatcca taaggcatct
     1081 gatgcgcgag gtttttcatg actgaagact ccacatccag ccactcgaaa gcatcgtccc
     1141 tgacgactta cttcagcgtg tggatcgctc tgctcgtcgg caccttcctc acctacgagg
     1201 ccgcgaaggt cgatctcgga gtcttcaacg cagcggttgc cttgaccatt gccacgacca
     1261 aggcgctcct cgtcgcactc ttcttcatgc acctcaaagg cgcgagcgaa aagctgctca
     1321 agctggttgt gatctgcacg atctttttcc tgatcatcct gctggcgctt tccatggctg
     1381 actacggcac ccgctcctgg agttgatgtg gaagagcggc ccttcacctc cgctaaaatg
     1441 ccaaaactcc ggcgggcctc aagtcgctga gaggcaaccg aggcactgcc ttcgcctcca
     1501 atctgcggta tactcgcgcc gtcgctgctg aaccgctccc cgcgaggtga cttatggcat
     1561 tttgcaattc ctgcggcacg agtctcgccc ccggaacaaa attctgctcc aaatgtggcg
     1621 ccgctatcgc cggatcaccg gtccctgccg cgacgcccgc cccacctgcc acaggcggta
     1681 gcagcgcttt gaagatcgtc ctgattgtcg tcgccgtgat cgccggtttg ggcattctcg
     1741 gtgttgccgg ttttggctat ttcgtttatc acgtcgccag aacagcgcac gtgcgccagg
     1801 aaggcaacaa tgtaaagctg gaaactccct tcggtagcgt cgaaacctcg caggatcccc
     1861 agaaagcggc gcaggacctg ggcgtcgaca tctatcccgg agcggaagtg cagaagaacg
     1921 gcgcctccga tgcaaccatc ggcaaacttc acaccgtcac cgctatcttt accagcagcg
     1981 actcactgga caaagtctgc agcttctaca agtcgaaatt tccgaatgcg atggtcgcga
     2041 cttcggacca aaaccactgc aacattgttt cgaacgacag aaagaacacg atcaccatca
     2101 acattcaggc cagcggcgat acgactaaaa tccagatcac caatatcaac cagaacgccg
     2161 cgtcgtcgaa ctgaccaccc gggcccatgc cagataaagt cgcagaattc gtatcggtcc
     2221 gagggttcag ggagaccgat gcattgggac ttgcggaggc cttgcgcgcg cgaattcgcg
     2281 gagacgtccg gttcgacccg ggcagccgcg cactctatgc caccgacggc tccaactacc
     2341 gtcaggtccc gatcggagtc gttcttcccc gcgacaacga tgatgtcctc gccacgattt
     2401 cgctctgccg cgaattcggc gctccgttgc tctgccgcgg cggtggcaca tcgcttgccg
     2461 ggcaatgctg caacgtcgcc gtcgtgctcg acttctcccg gtacatgtcg cggattctcg
     2521 agatcgaccc ggcccgccgc atcgcccgcg tgcagcccgg agtcgtgctc gatcaactcc
     2581 gcaacgccgc ggaaaaacat cacctgacct tcgcacccga ccccgccacc catgaccgct
     2641 gcaccatcgg cggcatgatc ggcaacaatt cctgcggcgt gcactccgtc atggccggca
     2701 agaccgacga caacatcgag gcgctcgatg tcgtcaccta cgacggcacg cgcttgcgag
     2761 tcggtgaaaa tctaatcgag acacgtaggg acagccgccc ccggctgtcc ggcggcccgg
     2821 aactgtcggg cagcgccgat cgagcagctc aaatctccag cccccttaag caaatcgccc
     2881 gcgagtacgc caatctcatc cgccaaaaat tccccaacat cccccgccgc gtctccggct
     2941 acaacctgaa ctacctgctg ccggaaaaca acttccacgt cgctcgggcg ctcgtgggat
     3001 cggaaggaac ctgcgtcacg atccttgaag ccacctgccg cctggtcgag agccctcccg
     3061 agcgcgtgct actcgtcctc gcctatcccg atatttatca atgtgccgac catgtccccg
     3121 aaatcatggc ccacaggccg atcggcctcg agggcttcga cgatctcctc gtcggctaca
     3181 cgcgcgcgaa aggcatcaac tccgaggggc tcgcgctgct ccccgaaggc aacggctggc
     3241 tcatggt
//
LOCUS       DASPUL010000359         6845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1289862, whole genome shotgun sequence.
ACCESSION   DASPUL010000359 DASPUL010000000
VERSION     DASPUL010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289862"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..980
                     /locus_tag="VEJ00_08660"
     CDS             375..980
                     /locus_tag="VEJ00_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXX01270.1"
                     /translation="MKRMLLVALLGLAMPLVAFASNSIDFTNSGGTLTGSNAGLSLTG
                     SELIAVNGLNNLGLVQGDLGTVTFSTGALISGSFQKGGQFAGGGSFIIQGNGTNGIPN
                     GNIFNGSFAGPVKWCMVTLANGTHNYTLMGAISGTWLNGGTVNGAAVELTINTGKGFF
                     NGSTSISSGDINVTVPEPSTLGLMGIGMVGLAGAIQRRRKA"
     gene            1137..1400
                     /locus_tag="VEJ00_08665"
     CDS             1137..1400
                     /locus_tag="VEJ00_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01271.1"
                     /translation="MKDIHEVLRQKQAKYAQLGKQIEMLQQAAEKLREVAPLLAENDD
                     EDNAVLAEVDDAAGQGDSMAAKAGASGGSTSSKGARPAAPRWP"
     gene            1431..2576
                     /locus_tag="VEJ00_08670"
     CDS             1431..2576
                     /locus_tag="VEJ00_08670"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXX01272.1"
                     /translation="MTLQSLLVSTDDAAADVLGRVLPTFGMALDRSSDPETTLVRIQQ
                     QKFDALIVDYDDPALAEQALEQARRLGTGCISIALVAEPSKVRDILSSGAHFVLYKPL
                     SEAAAQAGLRAAAALLSRERRRAFRVSVQAPVEITRADQRKADGILLDLSETGMDVLT
                     SEAQVPGSLLGFRFQLPDGALVIEAHGQVAWANPNGQTGVHFLDIPQEVLGQLQEWLK
                     AAATASGADPNEAVPHCKLSDLSLGGCYVETDAPFPERALIDLCLRTGELEVHSEGMV
                     RVTHPGHGMGVEFPSRTAEQRAQVGNVIDFLRNSPDTTPNLIISPRALVADLTQFEPA
                     PQAEGSAQTEELEDPLLELLRRGVSMQQDDFLSELHHQRHPQDVAPV"
     gene            3051..4646
                     /locus_tag="VEJ00_08675"
     CDS             3051..4646
                     /locus_tag="VEJ00_08675"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HXX01273.1"
                     /translation="MGAAAFAVEIPFFFRGTPSGHDVEFHLYSWLEVLSGWKQGIWFP
                     RWAAQAHFGYGEPRFIFYPPASWMLGALLSAIFPWTVAASIYIWLVLVLAGASMFMLA
                     RRWLDRRDAIFAAVLYAVNPYHLVIVYWRSAFAELLASCLIPLLLLLVLKAAEEGTKA
                     IVPLALLLAAAWLTNAPAAVMIHYSLALLTVLIAWQRRSARVFPVAAAAVALGAGLAA
                     FYLIPAIYEQRWVNITEAISAGSRPQDSFLFVHTTDAEHDAFNRIVSWIAVLEIVATF
                     VAAAAAKRWREGNRGLWNTLFCWAAGCTLLMLPVTAPIWRFLPKLEFMQFPWRWLLCL
                     SLMFAIFVTLGLRRQSLRAAFCVVAILVVAMVWRGVQAPWWDTAADLREMRDNVTDNL
                     GYEGVDEYTPLGAEATAVDKDARDVTVDGPARGAIHVLRWGPESKEFTAEVSAPDQLA
                     LRLFYYPAWRATVNGRVVTGRARVDTGQMLVPVETGMNRVQVTFARTWDRSAGGWIST
                     LSTLGVGICVFRKRRRQARALMR"
     gene            4697..5314
                     /gene="rdgB"
                     /locus_tag="VEJ00_08680"
     CDS             4697..5314
                     /gene="rdgB"
                     /locus_tag="VEJ00_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HXX01274.1"
                     /translation="MRRILIASSNPGKMRDFAGAAAEHGIEIASIPNFSSLPIVVEDG
                     LTFEDNARKKAEQYSRYVPGEIVVADDSGLEVDALNGAPGVHSARYAADEPHLADTNT
                     EDEANNARVLRELHSVPPEKRTARFVCVLAAARDGKMLASFRGTAEGIILDAPRGTNG
                     FGYDPLFYFPRIGKTFAELCAEEKAKYSHRGAAFRAFLEWCDLRV"
     gene            5716..6441
                     /locus_tag="VEJ00_08685"
     CDS             5716..6441
                     /locus_tag="VEJ00_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01275.1"
                     /translation="MASASTTAPQIKPGVAPLRAGQGYSFLLRRLHSLSGIVPIGAFL
                     VEHFISNSEALKGKEAYGATVQLLNSLPFVLGLELFLIWIPILYHGLYGLWIWYRGEG
                     NVVAYPWTGNWMYTAQRWTGIVALAYMVQHTYHLRFTGVHLATHPMESFAKVQNEFQN
                     PWMVAFYAVAIVAASWHFCYGMWLFAAKWGITTGERARRWFGYVCVVVALGLIAMGAV
                     SMYGFLSAPVQPLGSSTESIVMR"
     gene            6544..>6845
                     /locus_tag="VEJ00_08690"
     CDS             6544..>6845
                     /locus_tag="VEJ00_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXX01276.1"
                     /translation="MAAPKIIVVGGGLAGLAAVIKIAEMGGEVDLFSIVPVKRSHSVC
                     AQGGINAAKNLKGEGDSTWKHFDDTVYGGDFLANQPPVKDMCEAAPGIIDLLDRMGV"
BASE COUNT         1302 a         1926 c         2151 g         1466 t
ORIGIN      
        1 gttctcctcg accttgtctc gaccgaatgc ggcgcgagcg ttgagcctgg tcacgtcggc
       61 ctacagcatc gtcgtgggga gcattttcct gttggggtct gcggacaaac tgccttctct
      121 gacgggatga tggcagcgag ggatcgaccg gaagtcgcaa tatcctgttg caatgtatgc
      181 attagtttgc actcgaatct gttgaaaact gacgtttggt aaagatcaag ttcttaatat
      241 gcaaagtctt gcgacacttg caagtttggt catctggatg cactagacgg gccgggttgg
      301 actgtcgagg atatcgatcc tgccggtgcc ggttggggtt gttgtcgagc tcgtacagta
      361 aggaggcagt tatcatgaag cggatgttgt tggttgcttt gctaggtctg gccatgccgc
      421 tggtagcttt tgccagcaat tccattgact ttaccaacag tggcgggacc ctgaccggaa
      481 gcaacgcggg gttgagcctg accggctccg aactgattgc cgtaaacggt ctgaacaacc
      541 tcggtctggt tcaaggcgac ctcggcactg tcacgttttc cactggcgcc ctgataagcg
      601 gcagtttcca aaagggcggg cagtttgccg gtggcgggag cttcatcatt cagggcaatg
      661 gcacgaatgg cattccaaac ggaaacatct tcaatggaag cttcgcaggc ccggtgaagt
      721 ggtgcatggt gacgcttgcc aacggaacgc acaattacac cctcatgggc gcgatttccg
      781 gcacctggct caatgggggg acggtgaacg gcgcggccgt tgagctcacc atcaataccg
      841 gtaagggctt ctttaacgga agtaccagta tctccagcgg ggacatcaac gtcaccgttc
      901 cagagcccag caccctgggg ttgatgggaa tcggcatggt aggactcgct ggtgccatcc
      961 agcgcaggcg caaagcctga tttcgaacct ggttacggat ccgaggcccc gcccgaaaag
     1021 cggggccttt ttctatgttc ccagcctgac acgaagcgtt acccacgtaa tcagcgtggg
     1081 gggattaccg ttgacccatg cggcgcggcc ttcttaaatt gccagcaggg ggtagtatga
     1141 aggacattca cgaggtcctg cggcagaaac aggcgaagta cgcccaactt ggcaagcaga
     1201 tcgagatgct gcagcaggcg gcggagaagc tgcgcgaagt ggcgccgttg ctggccgaga
     1261 acgacgacga agataacgcc gttctggcgg aagtcgatga cgcggccggc caaggcgaca
     1321 gcatggcggc caaggctggg gcgagcggcg gttcgaccag ttccaagggg gcgcggcccg
     1381 cagcgcctcg ctggccctga actcgctcgg gctgccagcc cgattcgatt atgactctgc
     1441 agtctctact ggtctcgact gacgatgccg cggcagatgt gttgggccgg gtgctgccta
     1501 cgttcggcat ggccctggac cgctcaagcg atccggaaac aacgctcgtg cggatccagc
     1561 agcagaagtt cgacgccttg attgtcgact atgacgatcc ggctctcgcc gaacaggcac
     1621 tggagcaggc gcggcgcttg gggacgggct gcatcagtat cgccctggtg gcggagccat
     1681 ccaaggtccg cgacattctc agcagcggag ctcactttgt gctctacaag ccgctgtccg
     1741 aggcagccgc ccaggctggg ctgcgagccg cggctgcgct gttgagccgg gaacggcggc
     1801 gggcattccg cgtatcggtc caggctccgg tggaaatcac ccgggcggat cagcgcaagg
     1861 ccgatggaat tctgctcgac ctcagcgaaa ccggcatgga tgtcctcacc tcggaagcgc
     1921 aggttcccgg gtcgctgctg ggcttccgtt ttcaactgcc cgacggtgcc ttggtcatcg
     1981 aagcccacgg acaggtggca tgggcgaatc cgaacggaca aaccggagtt cacttcctcg
     2041 acattccgca agaggttctg ggccagctgc aggaatggct caaagccgcg gccactgctt
     2101 cgggcgcgga ccccaatgag gccgttcccc actgtaaact gagcgatctt agcctgggtg
     2161 gatgttatgt agagaccgac gccccgtttc ccgagcgggc tcttatcgat ctatgcctgc
     2221 ggacgggcga gctcgaagtt cacagtgagg gtatggttcg tgtcacgcat cccggacacg
     2281 gcatgggcgt ggaattccct tctcgtaccg cggagcaacg cgcacaggta ggcaatgtca
     2341 tcgacttcct gcggaacagt ccagacacga ccccgaacct gatcatttca ccacgagcac
     2401 tggttgccga tctcacacag tttgagcctg ctccgcaagc cgagggatcc gcacaaacag
     2461 aagaacttga ggatccgctt ctcgaactgc tgcggcgcgg cgtctcgatg cagcaggacg
     2521 atttcctcag cgaacttcac caccagcgcc acccgcaaga cgtcgccccg gtctgattca
     2581 ttcctgcccg tttcgggata tcgcgcagtc acttgccgtt tcggcgacct cattttggcg
     2641 ctcgaccgcc cgggcgcgac acaaagcctc cccaagcagg ttcggaacag gttttcttca
     2701 gaaggaaact cggcgggcag cgcagcagaa aaagagcatg tgggagcgag aggcttcggc
     2761 ggtggaccag ccgaagaatc gaagaacccg ggccccgtct ctggcccggg tccttcaact
     2821 catgtggtct gtaccacccc catttgcggc tacagtagcg cctcagcgtt gcgaaatgat
     2881 gtcagccata ttaaatgtgg gttaaatgta aactgggaaa aggtttcaaa agcgcattgt
     2941 tgtcgtgaag gaactccatt ctggaaaaat actctccacc tgcacaaccc tccagcgaat
     3001 cgcccggggc cgatcgaagg gattcactgt catggcgcgc ggcgctggcc atgggcgcag
     3061 ccgctttcgc ggtggagatt cctttcttct ttcggggcac gccatccggc cacgatgtgg
     3121 agttccatct ttactcctgg ctcgaagttc tgagtggctg gaagcaagga atctggtttc
     3181 cccgatgggc tgcccaagct catttcggct acggcgaacc gcgattcatc ttttatccgc
     3241 cggcgtcatg gatgctgggc gcactgctga gcgcgatctt cccctggact gtggcagcga
     3301 gcatttatat ctggctggtg ctggtgctgg cgggggcgtc aatgttcatg ctggcgcgac
     3361 gttggcttga ccggcgggac gccatcttcg cagccgtgct atatgcggtg aatccctatc
     3421 acctggtgat tgtttactgg cgcagcgctt ttgcggaatt gctggctagc tgcctgattc
     3481 ctctgcttct gctgctcgtt ctgaaagcgg ctgaggaggg gacgaaggcc atcgtgccgc
     3541 tggcgctgct gctggcggcg gcgtggctca ccaatgctcc cgcggccgtg atgattcact
     3601 actcgctggc actgctgaca gtcttgattg cctggcagcg tcgatctgca cgagtatttc
     3661 cggtcgcggc ggctgcagtt gccctgggcg caggtctggc cgcgttttat ctgatccctg
     3721 ccatctacga gcagaggtgg gtgaatatta ccgaggctat ctcggccggc tcccgtccgc
     3781 aggatagttt cctgtttgtc cacacgaccg acgccgagca cgacgcgttt aatcgaatcg
     3841 tctcgtggat tgccgtcctg gagatcgttg cgaccttcgt cgcagccgct gcggcaaagc
     3901 gctggcggga gggcaaccgc gggctatgga acactctttt ctgctgggct gcgggctgca
     3961 ccctcttgat gcttccggtg accgcaccaa tttggcggtt tctgccgaag ctggaattca
     4021 tgcagtttcc ctggcggtgg ctgctctgcc tgagcctgat gttcgccatc ttcgtcacgc
     4081 tggggttgcg gaggcagtcg ctgcgggcag cgttctgtgt tgttgcgatt ctggtggttg
     4141 ccatggtgtg gcgcggagtt caggccccct ggtgggacac tgccgccgat ctccgcgaga
     4201 tgcgcgacaa tgtcacggac aatctcggtt acgagggcgt cgatgagtat acgccgctcg
     4261 gagccgaggc gactgccgtt gacaaggatg cgcgcgatgt cacggtcgat gggccggcgc
     4321 gcggtgcgat tcacgtcttg cgctggggac cggagtcgaa ggagtttacg gcggaagtgt
     4381 cagcgccgga tcagctggct ctgcggctgt tctactatcc ggcatggcga gcgacggtga
     4441 acgggagagt cgtcacgggg agagcgcgcg tggacacggg acaaatgctg gttccggtcg
     4501 aaacgggcat gaaccgcgtt caagtcacct ttgcccggac atgggatcgc agcgcgggcg
     4561 gctggatctc cacccttagc actttgggcg tggggatctg cgtctttagg aaacgaaggc
     4621 ggcaagcgcg ggccctgatg cgttgatcct ttgtgcggct gccctgcccc tgtgattgtt
     4681 tttctcttga tcacctatgc gacgaattct gatcgccagc tcgaatcccg ggaagatgcg
     4741 cgattttgcc ggggccgcgg ccgaacatgg tattgagatc gcaagcattc ccaacttctc
     4801 ttccctgccc atcgtggtcg aagacgggct gaccttcgag gacaacgcgc gcaagaaggc
     4861 agaacaatac agccgttacg tcccgggaga aattgtcgtg gcggacgact ccggcctcga
     4921 agtcgatgca ctgaacggcg cccccggcgt gcattcggcg cgatacgcgg cagatgaacc
     4981 tcatctggct gacacgaaca ccgaagacga agccaacaat gcgcgagtgc tgcgagaact
     5041 gcatagtgtt ccaccggaga agcgcactgc acgattcgtc tgcgtgcttg ccgccgcgcg
     5101 tgacgggaaa atgctggcca gctttcgcgg aaccgccgaa ggaatcatcc tcgatgctcc
     5161 gcgtggaacc aacggattcg gatacgaccc gctcttctac tttccacgaa tcgggaagac
     5221 cttcgcagaa ttatgcgccg aggagaaggc aaaatacagc cacagaggag cggctttccg
     5281 agcgtttctg gagtggtgtg atttacgggt ttagttttcg accgttggaa ggaaatcaag
     5341 tgagtgttgc gtaacttgga tggatgtaag agaattaacg caaagagctg taaggaagct
     5401 gctatgacgg catgctggcg agcacagtcc ggacacctca cttgcaattg gtcgcaggcc
     5461 gccgagcgcg ttcaggaccg tccaagttgg atgcaggaat cttctgatgt tcgcagccca
     5521 tatctgccgc ccattcccga ttttgcaagt cacagtccct tcggcggggc cttctggttc
     5581 gagcccatca cgacgcgcgg tgcttccgag tagttccggc ggctcctgac tgaccgtcct
     5641 ttccttgact tcacttctcg cgcggccaga taatacaagg ttcttttttc gttcgcggac
     5701 ttataaggag cttcggtggc gtcagccagt actactgctc cccaaatcaa gccaggcgtg
     5761 gctccgctgc gggcggggca aggatattcg ttcctgctgc gccggctgca ctcgcttagc
     5821 ggcatcgttc ccatcggcgc ctttctcgtc gaacatttca tttcgaattc ggaggccctc
     5881 aaggggaagg aagcctacgg ggcaaccgtc cagcttttaa acagcctgcc gttcgtgctc
     5941 ggactggagc tgtttttgat ctggattccg attctgtacc acgggcttta tgggctgtgg
     6001 atctggtatc ggggcgaagg caacgtagtc gcttatccct ggacgggcaa ctggatgtac
     6061 acagcacagc gctggaccgg catcgtcgcg ctggcttaca tggtgcagca cacctatcac
     6121 ctgcgcttta caggcgtgca tctggccacg catcccatgg aatcgttcgc caaagtgcag
     6181 aatgagtttc agaacccatg gatggtcgca ttttatgcgg tggcgatcgt ggccgcgtcg
     6241 tggcatttct gttacgggat gtggctgttt gccgcgaagt ggggcatcac cacgggcgag
     6301 cgggcgcggc ggtggtttgg ctacgtttgc gtggtggtgg cgctggggct gattgcgatg
     6361 ggcgcggtca gcatgtacgg gttcttgagc gcgccagtgc agccgctggg ttcgagcacg
     6421 gaaagcatcg tgatgaggta gcacgagttt tgtaatttgc gattgacgat tgccgattct
     6481 cgattgccgg gccggcagcg aatccaatcg gcaatcgaaa atcgccaatc ggaaatcata
     6541 cagatggcag ctcccaaaat tatcgttgtg ggtggagggc tcgcggggct cgcggcggtc
     6601 atcaagatcg ccgagatggg cggcgaggtt gacctgttct cgatcgtgcc ggtgaagcgg
     6661 tcacactccg tgtgcgcgca gggcggcatc aacgccgcaa agaacctcaa gggcgagggc
     6721 gattcgacct ggaaacactt tgacgacacg gtctacggtg gagactttct cgcgaaccag
     6781 cctccggtga aagacatgtg cgaggccgcg ccggggatca tcgatctgct cgaccgcatg
     6841 ggcgt
//
LOCUS       DASPUL010000360         4801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_208210, whole genome shotgun sequence.
ACCESSION   DASPUL010000360 DASPUL010000000
VERSION     DASPUL010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208210"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VEJ00_08695"
     CDS             <1..483
                     /locus_tag="VEJ00_08695"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXX01277.1"
                     /translation="YLVPGWRIGWGMVSGDAAAVKSYTEGVHRLLRARLCANHPEQYA
                     IKPALEGPQDHLVEVRRKLRSRRDLTQKWCESTPRVSCVAPRGAFYAFPRIDIPESDE
                     VFVKELIRQKHVMVVHGSGFGQKPSTQHFRIVFLPDEQTLTKAYAGIVEFIRERYGSS
                     "
     gene            complement(496..972)
                     /locus_tag="VEJ00_08700"
     CDS             complement(496..972)
                     /locus_tag="VEJ00_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01278.1"
                     /translation="MRGCILALFSGLVLLGSLASGQETKPPQTQNSSYETLSNQFFDF
                     LKQGKSSEAVDFLFETNPALKKMTDDATQLKTQFASVETLMGSYVSHTKLVETTVGGM
                     FVYQHFFVAYERQPISVRIEYYKPGSKWLCYSLQFDGKLTDKVRSLSDERISIEAK"
     gene            1222..1977
                     /locus_tag="VEJ00_08705"
     CDS             1222..1977
                     /locus_tag="VEJ00_08705"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HXX01279.1"
                     /translation="MVDIHSHILPEVDDGPKTWDACVTMCRAAAADGITHMVATPHAN
                     DRYHYDRAYLEGLVAHLRQLVSGAPEILLGCDFHLSYDNLQDAYANPSRYVIGDTRYL
                     LVEFSNYAIPQGTTDSLLKLGDCGMTPIITHPERNPILRENLPRVVEWAERGCVIQMT
                     GSALTGFWGERTRAAAQWLLEHDAVHVLATDAHDVEKRVPILSTSRDAAAEICGDEVA
                     EALVETNPRAIISNQPLPYFPQPLQSSYKKSNH"
     gene            complement(2236..2856)
                     /locus_tag="VEJ00_08710"
     CDS             complement(2236..2856)
                     /locus_tag="VEJ00_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HXX01280.1"
                     /translation="MLRWTQTRNARFGAACALILACFSLPASARRQSSGSSDGAAKKP
                     AAHKPATKTTSPTHHATSSKASTATKAAGTTHATTAGKHSSRRKKSAHARGQQKIDSE
                     RAQQIQEALIREHYLSGEATGKWTPACEDAMRRYQADHGWQSKTVPDSRALISLGLGP
                     SKDHLLNPESAMTSGPAPPRTPTVTPVAQPTPAATNPVQSDPPSPQ"
     gene            complement(3051..4649)
                     /locus_tag="VEJ00_08715"
     CDS             complement(3051..4649)
                     /locus_tag="VEJ00_08715"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HXX01281.1"
                     /translation="MDSGAMWLRVKAHGWAYTLSILATLAVGILIGTVVSYGVKGKEG
                     QKSTDASPLTIPSSTTSGSGFSQQLSNAFAQVAKQLEPSVVNISTESTIKSPHRRRGQ
                     DDDDSGDGMDDFFNHFFGGPGGQNPFGPGDGSIRERSLGSGVIVDPKGYIVTNRHVVE
                     KADRIRVRFEDDPPGTQHEARVIGTDQETDLAVIKVDVDKALPAAKLGNSDSMQVGDW
                     VVAVGSPFGLSETVTAGIVSAKGRDIVPNRQFQTFIQTDAAINPGNSGGPLVNMNGEV
                     IGINTAILSETNAYAGVGFALPSKTVAEVYNQLISPDHRVARGSIGITFDSVENPAIT
                     RVYGAGTGVTISSVVAGSPADNAGLKIGDTITSIDGRKIAKGSELVGDIASRKPGSKI
                     EMGFIRNGKMQNASVTVADRAKLFAARFGDDQESEDNSTPKQSKFGVVVRKVTPEMAE
                     RLDMSVGKGVIVQDVKPGSFAEDVGLGRGDIILEINKQPVNSEDEFARIESAMKSGQD
                     VVFLVRQRGTTKQDGTIFDAGTLP"
BASE COUNT          898 a         1476 c         1417 g         1010 t
ORIGIN      
        1 tacctcgtcc ccggatggcg catcggatgg ggcatggtct cgggcgacgc cgccgcggtc
       61 aagtcgtaca ccgaaggtgt gcaccgcctg ctgcgcgcgc gcctgtgcgc caatcatccc
      121 gagcagtacg ccatcaagcc ggcgctcgaa ggcccgcagg atcatctcgt cgaagtgcgc
      181 cgcaagctgc gctcccgccg cgacctcacc cagaagtggt gcgaatcgac gccacgcgtg
      241 agctgcgtcg cgccgcgcgg ggcgttctat gctttcccgc gcattgacat ccccgaaagc
      301 gacgaagtct tcgtgaaaga gctgatccgc cagaagcacg tcatggtcgt gcacggctcc
      361 ggcttcggcc agaagcccag cacgcaacac ttccgcatcg tgtttctgcc ggatgagcaa
      421 acgctgacca aggcctacgc gggaattgtg gagttcattc gcgaacgcta tggaagcagc
      481 taaactgtcc gggcgttact tcgcctcgat cgatatcctt tcgtccgaca gactccgaac
      541 cttgtcggtg agtttgccgt caaactgcaa actgtagcag agccacttag atcctggctt
      601 gtagtactcg attcgtaccg agattggctg gcgctcatag gcgacgaaaa agtgttggta
      661 gacaaacatg cccccaacgg tggtctctac tagtttcgtg tgcgagacat aagaacccat
      721 gagagtctcc acagaggcaa attgtgtctt tagttgagtc gcgtcgtccg tcatcttctt
      781 taaggcagga ttcgtctcga agagaaagtc aaccgcttca gaactcttgc cctgtttgag
      841 aaaatcaaag aactggttgg agagtgtctc gtacgacgag ttctgcgtct gtgggggctt
      901 ggtttcttgc ccgctcgcaa ggctcccgag caaaacaagg ccagagaata gggcgaggat
      961 acaacctctc atttggttct cctctagact gcacgatatc cgggcactgc gattctaccg
     1021 ggaaagacaa gaggaatgga taggtctcgg gattgcatat cacggccaac atcgccctcc
     1081 taggcaggac cttctcacag tctgcgctca ggctctcgtt ctttctcttt tcccccgaat
     1141 ctgtttaaat cgagaattaa gcaacttcag cggcgtgctg gtgttctcga aattgtgtcc
     1201 tgaaatagtg ttctcacaat tgtggtcgac attcactctc acatactgcc tgaggtagac
     1261 gacggtccca aaacctggga cgcctgcgtc accatgtgcc gcgctgcagc cgctgacggc
     1321 atcacacaca tggtggccac gccgcacgcg aatgaccgct accactacga ccgcgcgtat
     1381 ctggaagggc tcgtcgccca cctgcggcaa ctcgtttccg gagcaccaga gattcttctg
     1441 ggctgcgact ttcatctttc ctacgacaac ctgcaggatg cctacgccaa cccctcgcgc
     1501 tacgtgatcg gcgacacgcg ctacctgctg gtcgagttct cgaattacgc catcccgcaa
     1561 ggaacgaccg attccctgct caagctgggc gactgcggga tgactccgat catcacgcac
     1621 cccgagcgaa atcctattct ccgtgagaat ctgccccgcg tcgtggagtg ggccgagcga
     1681 ggctgcgtga ttcagatgac cgggtcggcg ttgaccggtt tctggggtga gcgcacgcgc
     1741 gccgcggcgc agtggctcct tgagcacgac gccgtccacg tgctcgccac cgatgcccac
     1801 gacgtggaga agcgcgtccc gattctctcc acctcacgcg atgccgccgc cgagatctgc
     1861 ggtgacgagg tggctgaggc tctggtcgaa accaaccccc gcgccatcat ctccaaccag
     1921 ccgttgcctt actttcccca gccgctgcag tccagctaca aaaagagcaa ccactagcgt
     1981 cgggcgacaa gcgtcgggcg gtcgccgaat ccggatgctg ctcgcttcgc acaatgcccc
     2041 gcgcagcgat ttccgagccc tccggtgtgt tcaggagtgc caagtggagt ttcccggtcc
     2101 ggcaggaatt tagtgtgctg cagcgaaggg ggaatcgatt taaccgctat atgcaagctg
     2161 ctctgtatca atcacttaca tggtacattt gtcacatggg ttgaagctcg aaaaagctgc
     2221 cgcttctcga ctggtctact gcgggcttgg cggatcagac tgaacggggt tcgtcgcggc
     2281 tggtgtgggt tgtgcgaccg gggtcacggt cggggttcgt ggtggcgccg ggccgctggt
     2341 catggcactt tcgggattga gcaggtgatc tttgctgggg ccgaggccaa ggctgatcag
     2401 tgcccgcgaa tcgggcacgg tctttgactg ccaaccgtga tcggcttgat agcggcgcat
     2461 cgcgtcttcg cacgcagggg tccacttgcc ggtcgcctcg ccgctcaagt agtgctcgcg
     2521 gataagggct tcctggatct gctgggcccg ctcggagtcg attttctgct ggccgcgggc
     2581 gtgcgccgat ttcttccggc gcgaagaatg cttgccggcc gtggtcgcat gggtcgtgcc
     2641 cgcagccttg gtcgccgtcg atgccttgga cgaagtcgcg tgatgcgtgg gtgacgtggt
     2701 ctttgttgca ggtttgtgag ctgcgggctt tttcgccgcg ccatcgctgc tgccggagct
     2761 ctgccggcgc gctgacgctg gaaggctgaa acaagccagg atcaacgcgc aggccgcgcc
     2821 gaatcgcgca ttccgggttt gtgtccaacg cagcaaaggt tccccagggc aaaagccgca
     2881 tcctgctcgg gtgcataacg tggctgaggc gaggctcagg ccatcgactt cccgaccaat
     2941 cctgccgagg cttgcggttc cggcaaaaat aaaaagccca accggacgct ggcattgtaa
     3001 tcatccgcga tcgggagtta cagcgccagc tcctattggg ctctttgagg ttacggaagt
     3061 gtaccggcgt cgaagatggt accgtcctgc ttggtcgtgc cccgctggcg aaccaggaac
     3121 accacatcct ggccgctctt catggcggac tcgatccggg cgaactcatc ttcgctgttg
     3181 accggctgct tgttgatttc caggatgatg tcgccgcggc ccaggccgac gtcttcggcg
     3241 aacgagcccg gtttcacatc ctgcacgatc acgcccttgc ccacggacat atcgagccgc
     3301 tcagccattt ccggggtcac cttgcgcacg accacgccga acttgctctg cttgggcgtg
     3361 ctgttgtctt cgctctcctg gtcatctccg aagcgcgcgg caaacagctt ggcgcggtcg
     3421 gcgacggtca cggatgcatt ctgcattttg ccgttgcgga tgaagcccat ctcgatcttc
     3481 gatcccggct tgcgcgaggc gatgtcgccc accagttccg aacccttggc gatcttcctg
     3541 ccgtcgatgc tggtgatggt gtcgccgatc ttcaatccgg cattgtcggc cggactgccg
     3601 gccaccacgc tggagatggt cacgcctgtg cccgcgccgt agacgcgcgt gatcgcggga
     3661 ttctccaccg agtcaaacgt gatgccgatc gagccgcggg ccacgcgatg atcgggggaa
     3721 atcagctggt tataaacctc ggctacagtc ttcgagggca gggcgaaacc gacaccggcg
     3781 taggcgttgg tctcgctcag aatcgcggtg ttgatgccga tgacttcgcc gttcatattc
     3841 accagcggac cgcccgagtt tccgggattg atggcggcat cggtctgaat aaaagtctgg
     3901 aactggcggt tgggaacgat gtcgcgtccc ttggccgaga caatgcccgc cgtcacggtc
     3961 tccgacaagc caaacgggct gcccacagcg accacccagt cgcccacctg catggaatcg
     4021 gagttgccca gcttcgcggc aggaagcgcc ttgtccacgt ccaccttgat caccgccagg
     4081 tcggtttcct gatcggtgcc gatcaccctc gcctcatgct gggttccggg agggtcatcc
     4141 tcgaaccgta cccggatgcg gtcggccttt tcgaccacgt gccggttggt gacgatgtat
     4201 cccttcggat cgacgatcac gcccgaaccc agcgagcgct cgcgaatcga gccgtcaccg
     4261 gggccgaacg gattctgtcc gcccggacca ccgaagaagt gattgaagaa atcatccatg
     4321 ccgtcgccgc tgtcgtcgtc atcctggccg cgccggcgat gcggagactt gatcgtcgat
     4381 tcggtgctta tgttgaccac gctcggctcc agctgcttgg ccacctgggc aaaagcattg
     4441 gaaagctgct gcgaaaagcc gctccccgag gtagtggaag aagggatggt cagaggcgac
     4501 gcatccgtcg atttctgccc ttccttgcct ttgaccccgt aggagacgac cgtcccgatc
     4561 agaatgccga ccgcaagggt cgccagaatg cttagtgtgt aggcccagcc gtgagccttg
     4621 acgcgcagcc acattgcgcc cgaatccata gatctacttc cctccggaac ttgataaccc
     4681 cacccatttt acctgtttgt gaagcgcttg gactaccgcc gtgtcgagag ccataacacc
     4741 tgccgggggg ctttcttcct tagacgcgca aaaggggaaa agcgtttgat ggagagtaac
     4801 a
//
LOCUS       DASPUL010000361        14175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1019033, whole genome shotgun sequence.
ACCESSION   DASPUL010000361 DASPUL010000000
VERSION     DASPUL010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 14175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14175)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14175
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019033"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VEJ00_08720"
     CDS             <1..276
                     /locus_tag="VEJ00_08720"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HXX01282.1"
                     /translation="NAIMLGKDSFYPPHVAFYLAHEIAHIALGHLGEHPMVVDLETEE
                     PTATDQDAEENKADEFALELLTGHPRPTVLPTFGGSSPRVLASRKSW"
     gene            complement(612..1739)
                     /locus_tag="VEJ00_08725"
     CDS             complement(612..1739)
                     /locus_tag="VEJ00_08725"
                     /inference="COORDINATES: protein motif:HMM:NF005313.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXX01283.1"
                     /translation="MPSVNKVLILGGGIGGLALSIGLRRGGIDVEIAEIKEQWTVYHV
                     GIIAQSNLVRAMVALGIADACVAAGFSYQGVRFCDANGNVITDSPGVKLAGPQYPAYL
                     GLTRPALHDVLSTASKKSGVNLRLGTTVAELLQSDKKVVVKFTDGIPREYDLVVGADG
                     VHSQIRPMVFGSQLTPRFTGEGVWRYNVPRPPDVDYGSIYATREGPKAGLIPLTHETA
                     YIFRIGPEPGNLRFPKSELADIMRERLKPFGGPIGELARGITDPNLVVYRPLESVLVP
                     APWYRGRVALIGDAAHSITPHLGQGAAQAVEDAVVLAEELQKDSPLEKSLDSYVKRRY
                     ERCKFILEASLQIGEWEMHPDPKADVANLMAKVGQTVVQPI"
     gene            complement(1840..2892)
                     /locus_tag="VEJ00_08730"
     CDS             complement(1840..2892)
                     /locus_tag="VEJ00_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXX01284.1"
                     /translation="MTMHSSTFALGTFAVDGRPPFPALVVSERVAPIHALKPVLEHLG
                     SAPLSSSSILGLLENWEESFEVLRRMADVFQQDSPKNGRWRDVFVPSEKLSFRPPVDL
                     PRQVFCAGANYRKHVIDLIVDQPSSADKNLSREERHARAVRLMDERAAQGAPFVFSKP
                     PSAITGPYDPVILPRHAAQPDWELELAAIIGKPARHVRREEALDYVAGYTIVNDITNR
                     DLVFRKDTGAMGADWIAAKGSPSYLPTGPYLVPAAFVSNPQNLQITLKLNGQVMQDES
                     TADMIFSVVRIIEFLSTHVQLWPGDLIATGSPAGNGTHYNRFLKPGDVMEGSITGLGT
                     QRNPCIAEEAGTHAAS"
     gene            complement(2920..3939)
                     /locus_tag="VEJ00_08735"
     CDS             complement(2920..3939)
                     /locus_tag="VEJ00_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXX01285.1"
                     /translation="MSRHILSHLAHVELLTPQLEESVAFFTNTLGLVESARTGESAYL
                     RCWGEHYHHSLVLTASAQPGLGHAAWRADGSDELNEAAARIEASGILGEWRDKSVGHG
                     RAYRFRGPGGHTHEVFWEVDRFDAPADLRSTYPDRPQRYVGRGIAPRQIDHVTLNTQD
                     VMKTCEWYRDTLGFRFMGYTVLDQDPGTAVFGVLTMNEKSHDLGFGGDFSKIPGRFHH
                     VAFWVPSYEDLLRAADMLLESGTPIEFGPGKHGIGEQGYLYFRDPVGLRVELNTGGYR
                     NYIPDWQPTVWRPSQGSHVMFRNLEYGPKSFMEAMPPAPEPAIPEPDVTPVTASAANR
                     GARQD"
     gene            4061..5023
                     /locus_tag="VEJ00_08740"
     CDS             4061..5023
                     /locus_tag="VEJ00_08740"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXX01286.1"
                     /translation="MKLSQFDLNLLVALDALLTEKNVTRAGMRIHLSQSATSAALARL
                     RNSFQDQLLVRVGGKMVLTPMAQSLVKPVRNLLLQAQATIATQARFEPATTRRRFSVA
                     VSDYATTVFMADALKNLQHLAPHLTFELVPTSERAVEALEAGALDFLIAPEVFASKTH
                     PKMPLFEDTHTVIAWTRNRSVRSKLSLDEYLSLGHVAVHVGEGAGPNFDELFLRRLKY
                     KRRAEVIAHSFDVVPHLVVGTNRIATVATRLARKYARFMPLKLVPLPVHVPPMTEVLQ
                     WHKFHDRDTAYVWLRGVLIDQAARLLGPPSDPGNGRAAVDRSKT"
     gene            complement(5228..6700)
                     /locus_tag="VEJ00_08745"
     CDS             complement(5228..6700)
                     /locus_tag="VEJ00_08745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXX01287.1"
                     /translation="MTAVNEDIEKDIDLLQAEDEAEVVPSAAATQEEPAYAAESELSP
                     TESLVSADPVALYLRETGRRRLLEREEEVALAKRIERGQARTLKAISRSPVVWREITA
                     MAVDLRYQNCRIDEVIECGDEELTPRQLESKTRKTLEAFDKITRLSKNAARQAKQLAR
                     FSKSNRPALVHARYRVARTAVKISELVRSIQFSAAERTRLSGTIQLALDTASSAPRAE
                     LKRLEAVPGFSAKDLERTLEMIRRGETETERAKNELAEANLRLVVSVAKKYQNRGVDL
                     LDLIQEGNIGLMKAVEKFDWRRGFKFSTYATWWIWQAVTRSISAQARTVRLPVHMIEV
                     INRMARTKGELAKQLNRNPSLEEIAQRIGLPAKKVEALMRSAQETLSLDSPVGDDEES
                     HLGDLIENAASISPSDAVMDLDVKERTSSVLKMLSPREQAIIRKRFGIDGEAEQTLEQ
                     VGQALGLTRERIRQIEAGVMRTLRKSGDIQQLRTYLRRAS"
     gene            complement(6912..7367)
                     /locus_tag="VEJ00_08750"
     CDS             complement(6912..7367)
                     /locus_tag="VEJ00_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01288.1"
                     /translation="MRKIGGRQSYSRSLRINLASRREEELFKWFLACLLFGKPIQTEI
                     AEQAYRELVGAGLTSADAIMRAGWDKLVRLLDRAHYVRYDFSTATKLLEVCQELRRRY
                     GTVTKLLSSSRTTSELSRKLQEFKHVGPVTARIFLRDIRPVWFAKTGRR"
     gene            7616..8548
                     /locus_tag="VEJ00_08755"
     CDS             7616..8548
                     /locus_tag="VEJ00_08755"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HXX01289.1"
                     /translation="MDLAHLGHLELLTPKPDETLKFFVNVMGMTESGREGESVYLRAY
                     DDYERHSLKLTASKLPGMGHVAYRARSPQALERRVAALRGSGFEFGWTDGDLAHGPAF
                     VCKDPDGHKIELYYETQWYEAPPELRPAMKNQAQKYPARGMNVRRLDHWNGLAVDIEA
                     NRAFFEKYLGFRLTEQIVLNDGSEAAMWMTCTNKSYDFAYTRDHTGTKGRFHHITYAV
                     NSREEVLIAADIALENGVYIETGPHKHAIQQTFFLYLYEPGGNRVEVANATARLILAP
                     DWKPIRWSEAERKKGQAWGLKTIESFHTYGTPPV"
     gene            complement(8618..9088)
                     /locus_tag="VEJ00_08760"
     CDS             complement(8618..9088)
                     /locus_tag="VEJ00_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01290.1"
                     /translation="MGEVTHDQANLLLRLYELRREPRMREAREWYFASFNPTSLEDMN
                     KQAPPGSAGNASVRMVISYWEMAASMVNRGLIDEDFFFENTGEQWAVWGRIKALVPAM
                     RERSKNPHQYANLEKHVNRFEAWREKRAPGSGEAMRQMMVQMQQAMKQSKSAGD"
     gene            complement(9145..10002)
                     /locus_tag="VEJ00_08765"
     CDS             complement(9145..10002)
                     /locus_tag="VEJ00_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXX01291.1"
                     /translation="MTSSLAHWMEIAGTRGARILVIAVIAFVLTRILKALTTRVIDIA
                     KVQARGARMREQQTRTLAGLLYSVGVAIIVAVATLMVLPEFGFDVTPFEAAAAVGSLA
                     FGFGAQNLVKDLINGFFIVLEDQYVVGDLIQLNGETGRVEHLTLRRTVLRNVSGAIVT
                     IPNSLVGQVANLSRDWSQAFVDVTVPSDELVGRALATLEKIAGDFRNDADWSPALLDG
                     PRVLGVESLSLEGTVLRLQVRTALNRKDDVARELRRRIKLGFEESRIPLRQAHQVELH
                     GAVAAAQSS"
     gene            10149..10225
                     /locus_tag="VEJ00_08770"
     tRNA            10149..10225
                     /locus_tag="VEJ00_08770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10183..10185,aa:Arg,seq:ccg)
     gene            complement(10317..>14175)
                     /locus_tag="VEJ00_08775"
     CDS             complement(10317..>14175)
                     /locus_tag="VEJ00_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HXX01292.1"
                     /translation="ILGTINNTGSFQVAANGNPTFLSMSGAVTLTGGGTVVMSIGGNG
                     NSPYIRQDTAGSSLTNSGNSISGPGGLTIPAYMQTAGFIQIPSGVSDSVTTFSISGGI
                     AQVDGTLSVSSGVSTSGTGVISGTGSLSSNTTNAGVIEAGDVPSPGVLNIPSSYTQNS
                     TGSFEVAIGGTTVGTQYSQVKVAGAASLAGALSVRLIGGFAPSLGNSFTILTASAIAG
                     QFSSINSPALPSGLAWSVAYNSTSVVLSVVSASPTSYTLTIAEPGSGSGTVTDDLGQI
                     NCVDTAGVVTGTCSASYQSGTVVVLTATPAVGTAFNGWSTCGGTGPCSITMNSAQTEQ
                     ATFGPVIPNYAVTITEIGTGTGSVTDNLGGINCSESNGVVTGTCSASYASGTQISLNE
                     SATSPSTFGGWGNACFSSGTGTSCNLTVSSALNVTANFIPPPVSVDLPFNAGSNVSQQ
                     ASFDCPSNPTPTPGNPCTDANAHAFQLQLSQVNSSFNLTVTATEVPPSQFDGLCEVGN
                     TVLNDFDCRFSTFFGYGTDPNGNTITPFCYPYANGNCVHYDVYVGTPGNVPDPGSYSG
                     PVNWKITFNNGTFTPPAPYWTGSSPRLYEDPDSAQSPSDAIGTVCTELMTIGGVAQNY
                     SCQFEFDITTFFDSSEPVDPGIGGTTKHLSDVVVAFPPTTSGQLSVTSTPDAASVNAG
                     STIGFTITVTNIGPGTEPDVTLNDPLPAGASWSLSTYTGPGSCGITGSAGSQVLACTF
                     GDLAANASATLHVSGASAAAGSYVNVATVASVVNEQPTQQVLSAAGVTVEALATSFSG
                     LTASQSIPFGTSSVNLTGTLSAPGPTYPSSGETVSVTINGSVQSTTLGANGVFSLTFP
                     TAAIPPSGTPYTITYSYAGDTDFAPATDTSTTLTVTQQAQTIAFTGAPETAVYNSSFP
                     VSATATSGLAVTITASGACSINGTTVTMISGTGTCQLAANQAGNADYSPAPQQAASVT
                     AEKASTTTTITSNTPNPSVTGQAVTISVSVTGNGTPTGSVQVSASTGESCTVTSLTAG
                     AGSCQITFQTVESPMLTAVYSGDTNFSTSTSLSVQQTVTASTSTLKISPTSVDFGNVN
                     FGAFGVQSITLTNTGTSTINIRNIAISQGNLNEFFAFSPFCNFPLSAGRSCIIFVTFF
                     PAPHQSDTQSATLIITDSAAGSPQSVPMTATIINPQASLNVHLLTFGTQKANTTSAAK
                     SVTLTNTGTTPLNLKDVDIFGDFAIASGTTCTDGSTVLPSANCIIYITFTPQSKGQRS
                     GRLSIRDNALFSPQEILLFGTGN"
BASE COUNT         2842 a         4170 c         4186 g         2977 t
ORIGIN      
        1 aatgccatca tgttaggtaa agattcgttc tacccacctc acgtggcgtt ttatctggcg
       61 cacgagattg cgcacattgc tctcggtcac ctcggtgaac atcccatggt ggtggatctc
      121 gaaacggaag aaccaaccgc cacggaccaa gatgcagaag aaaacaaggc tgatgaattc
      181 gctctcgaac tacttactgg acacccgagg ccaacagttc ttcccacgtt cggcgggagt
      241 tcgccacgcg tcctcgcctc cagaaaatcc tggtaggagg cacgcgcttc ccgcgcgtaa
      301 cccaactccc caaaacgcac ttccgatggt ttggcgtcat cgaccagtga caacggcaag
      361 aagcctgtcg tttggttcgg cgcattggga tctgctttaa ggaaaggttg cgcccgcagc
      421 aacggatatt gccaactgat ccagagtcgc cgtccccccg gctcgccgtc cgggacgcgc
      481 cggagccggc tacccagcgc agatccgagc gttcggaaaa cctcttcgac gctatccaac
      541 gcaatgctac cgacgaagtg cactttgtcg ggaatcatgt tgctctcctt ccgagatgcg
      601 gaaccagtga ttcagattgg ctgcacgacc gtttgcccta ctttcgccat gagattcgcc
      661 acatcagcct tcggatcggg atgcatctcc cactcgccga tttgaagcga ggcttcgagt
      721 ataaatttgc aacgctcgta ccgacgcttc acataggaat cgagtgattt ctcgagcggc
      781 gagtcctttt ggagctcttc cgccagcacg accgcatctt cgacggcttg tgccgcgcct
      841 tgcccaaggt ggggcgtgat cgaatgagct gcgtcgccga tcagcgccac tcgtccgcga
      901 taccacggtg cggggaccag cacggattcc agcggtcggt agacgaccaa gttgggatcg
      961 gtgattccgc gagccaactc accgatcggg ccgccaaacg gtttcagtcg ttcgcgcatg
     1021 atatcggcaa gctcgctctt gggaaaccga aggttgcccg gttccggccc gattcgaaag
     1081 atgtaggccg tttcgtgcgt gagtgggatc agtcccgcct tcggaccctc gcgcgtggcg
     1141 tagatcgagc cataatccac gtctggcggg cgcgggacgt tgtatcgcca gacgccttct
     1201 cccgtgaacc ttggcgtgag ttgactgccg aacaccatcg ggcgaatctg ggaatgaacg
     1261 ccgtcggcac cgacgacgag gtcgtattcg cgggggatgc cgtctgtaaa tttcaccaca
     1321 accttcttgt cggattgcaa aagctccgca accgtagtac ccagtcgaag attcacgccg
     1381 gatttcttcg atgccgtgga aagaacgtcg tgcaatgcag gtcgcgtgag gccgagataa
     1441 gccgggtatt ggggaccggc gagcttcacg cccggtgaat cggtaatcac gttgccgttg
     1501 gcgtcgcaaa atcgcacgcc ctgataagaa aatcccgcgg caacgcaagc gtcggcaatt
     1561 ccgagggcta ccattgcgcg caccaaatta ctctgtgcaa tgatgcccac gtggtagaca
     1621 gtccactgct ccttgatttc cgcaatctcg acgtcgattc cacctctgcg caggccaatg
     1681 gacaacgcca agccgccgat tccgcctccg aggatgagca ctttgttgac gctgggcata
     1741 atctcccgaa tttcaagccg tcactgttgt tcgagcgtca gcagatggca gtggacgtcg
     1801 accgtgagcg acttgcggcc gccctttttt gccccgcctt cagctggctg catgggttcc
     1861 cgcttcttcc gcgatacatg gattgcgctg ggtgccaaga cctgtgatcg agccctccat
     1921 cacgtcgccc ggcttgagga atcggttgta atgggtaccg ttcccggcag gcgatcctgt
     1981 cgcgatcaaa tcgccaggcc agagttggac gtgggtcgac aagaattcaa tgatccgaac
     2041 cacgctgaag atcatgtcgg ctgtgctttc gtcttgcatc acctgcccgt tgagcttcag
     2101 ggtgatctgc agattctgtg gattggagac aaaagcagcc ggcacgagat acgggcctgt
     2161 gggcagatag ctgggcgaac ctttggctgc gatccagtct gctcccatcg caccagtatc
     2221 tttgcgaaag acaaggtcgc ggttggtgat gtcgtttacg atcgtgtatc cggcgacgta
     2281 gtcgagggct tcttcgcggc gcacgtggcg cgctggcttg ccaatgatcg cagcgagctc
     2341 gagttcccaa tccggctgag cggcatggcg aggcaggatc acgggatcgt acgggccggt
     2401 aatggccgat gggggtttag agaaaacgaa aggcgcaccc tgcgcagccc tctcatccat
     2461 gagcctgacc gcgcgggcat gcctctcttc acgactcaaa ttcttgtccg cactcgaagg
     2521 ttggtcgacg atcagatcga tcacatgttt tcgatagttc gctcccgcgc agaagacctg
     2581 ccgaggcaaa tcaactggcg gccgaaaact gagcttctca ctcggtacaa aaacatcccg
     2641 ccaacgcccg ttcttggggc tgtcctgttg aaagacgtcc gccatccgtc gcaggacctc
     2701 gaaactctct tcccagtttt ctagaagtcc gaggatcgag gacgaactca acggcgcgga
     2761 tcccaggtgt tcgagcaccg gcttgagagc gtgaatcggc gcaactcttt cgctgaccac
     2821 caatgcagga aatggcggcc ttccgtcgac ggcaaatgtg ccgagcgcaa aggtggagct
     2881 gtgcattgtc atcacgaatt gtctctggcc gacatcgcct caatcctgcc gcgcgccccg
     2941 attcgctgcg ctggctgtca cgggtgtcac atcgggctct ggaatcgcgg gttcgggcgc
     3001 cggaggcatt gcctccatga atgatttggg cccgtattcc aggttgcgga acatcacgtg
     3061 agatccctgc gatggtctcc agactgtcgg ctgccaatcg gggatgtagt tgcggtaccc
     3121 gcccgtgttc agctcgacac gcagacccac cgggtctcga aaatacaagt agccctgctc
     3181 accgattccg tgcttgccag ggccgaactc aattggagtg cctgattcta ggagcatgtc
     3241 agctgcacgc aaaagatcct catacgatgg gacccaaaaa gcgacgtgat ggaatcgtcc
     3301 ggggatcttc gagaagtcgc cgccgaatcc caaatcgtgc gacttttcgt tcatagttag
     3361 aacgccgaat acagcggtgc ccgggtcctg gtcgagaacg gtgtaaccca tgaagcgaaa
     3421 cccaagagta tcgcgatacc actcacaggt tttcatgacg tcctgcgtgt tcaaggtaac
     3481 gtgatcgatc tgccgcggag caatgccccg accgacgtat cgctgcggac gatccggata
     3541 cgtcgatcta aggtcagcag gtgcgtcgaa gcgatcaacc tcccagaaga cttcgtgcgt
     3601 gtgtccgcct gggccgcgaa accggtacgc gcggccgtgc ccaaccgatt tgtcgcgcca
     3661 ctcgccaagg atgccgctgg cttcaatcct cgccgccgcc tcgttgagct cgtccgagcc
     3721 atccgcgcgc caagcggcgt gaccgagacc aggctgggcc gacgcagtaa gcactaagct
     3781 gtgatggtag tgttctcccc aacagcgtaa gtacgccgat tcgccggtac gggcgctttc
     3841 gacaagcccg agggtattcg tgaaaaaagc gaccgattct tcaagctgcg gcgtcaataa
     3901 ctctacgtgc gcgagatgag acaggatgtg tctagacata tgggcctcgc ttcaatgacg
     3961 catgacttgc atgaccagcc acgatagacg cattatttga tatttgccaa atcgcttgtc
     4021 ggcatatgct gtatccacat gacggatagt tggtgtcccg atgaaactct ctcaattcga
     4081 cctcaacctt ctggtcgctc tcgacgccct gctgaccgaa aaaaacgtga cgcgtgcggg
     4141 catgcgcata catttgagtc agtctgctac cagcgccgca ctcgcgcgat tgcgaaattc
     4201 ctttcaggat caattactgg ttcgcgtggg cggaaagatg gtattgacgc cgatggccca
     4261 gagcctcgtc aagccggtac gaaacctttt gcttcaagcg caggctacca tcgcaacgca
     4321 ggctcggttt gaaccggcga ctacgcgccg gcgtttttcg gttgccgtct ctgattacgc
     4381 gaccaccgtt ttcatggcgg atgctctgaa aaatcttcag cacctggcgc cgcacctcac
     4441 cttcgagttg gttcccacga gtgagcgggc ggtggaagcc ctggaggcag gggccctcga
     4501 ctttctcatt gcacccgaag tgttcgcatc gaagacccat cccaagatgc ctctctttga
     4561 agacacgcac acggtaattg cctggactcg gaatcgaagt gtgagatcta aactgtcgtt
     4621 ggacgaatat ctgagtctcg gtcacgtcgc cgtgcacgtt ggcgagggag ccggaccgaa
     4681 cttcgatgag ctgttccttc ggcgcctgaa gtacaagagg agagccgaag taatagcgca
     4741 cagcttcgat gtcgtccctc acctcgtcgt gggcacgaac aggattgcca ctgtcgccac
     4801 gcgcttggca agaaagtacg caagattcat gccgctaaaa ctagtcccac tgccggtcca
     4861 cgttccgccc atgacggaag tacttcagtg gcacaagttt cacgatcggg acacagcgta
     4921 cgtttggctt cggggagtat tgattgacca agcggcgaga ttgctcggcc ctccttctga
     4981 tccgggaaat ggccgtgccg cagtcgatcg gagcaaaacg tagcccgccg cccctccaac
     5041 ctatttcacg atggtgctgc gactttgcgc actcaggctc tttcccgccc gaagtggacg
     5101 actcgatttt gaggagcttg cgagagcggc gaacgaagcg acggcatccg ctgttttggg
     5161 agagaaacgc tacgcagatt cgagaaccag tttcaacccc agcgcggggt ccgggtaaat
     5221 tccgcgatta ggacgcgcgc cggagatacg tgcgcaattg ctgaatgtcg ccagacttcc
     5281 gaagggtacg catgacccct gcttcgatct gtcgaatgcg ctcgcgcgtg aggccaagcg
     5341 cctgaccgac ctgctcaagg gtttgctcgg cttccccgtc gatgccgaag cgcttcctga
     5401 tgatggcctg ttcgcgcgga gagagcatct tgagaacgga agatgtccgt tccttcacgt
     5461 cgagatccat tacggcgtcc gacggagaga tgctcgccgc attttcgatc aagtcgccga
     5521 ggtgggattc ctcgtcgtcc ccgaccggcg agtcgagtga cagcgtctcc tgagcggacc
     5581 gcatcaatgc ttccaccttt ttcgctggca aacctattcg ctgcgcgatc tcttccaagc
     5641 tcggatttcg attgagttgc ttcgcaagtt cacccttggt tcgcgccatc cggttgatca
     5701 cttcgatcat gtgcaccggc aaacggaccg tgcgtgcttg ggcggaaatc gagcgcgtca
     5761 ccgcctgcca gatccaccag gtcgcgtagg tagagaactt gaaaccgcgt cgccaatcga
     5821 acttctcgac cgctttcatc aggccgatat tgccttcctg aatcaggtcg agcaggtcga
     5881 cgccgcgatt ctggtatttc ttggcaaccg acacaaccag acgcaagttt gcttctgcca
     5941 gctcgttctt ggctcgctcg gtttcggtct cgcccctccg gatcatttcc agtgttcgct
     6001 ccagatcttt cgcgctgaat ccggggactg cttccagccg tttcagctcc gcgcggggag
     6061 cgctcgatgc tgtgtccagc gccaattgga tagtgccact caagcgagtc ctctccgcag
     6121 cactgaattg gatcgaccgc acgagttcgg agattttcac cgccgtgcgc gccacgcggt
     6181 accgagcgtg gacgagagcc ggcctgttcg atttcgagaa gcgcgccaat tgcttggcct
     6241 gtcgcgctgc gttcttggag agtcgggtga tcttgtcgaa cgcttcgaga gtcttccgag
     6301 tcttgctttc gagttgtcgc ggcgtgagtt cttcgtcgcc gcattcgatg acctcgtcga
     6361 tgcggcagtt ctgataccgc aagtccaccg ccatggcagt aatctctcgc cacactacag
     6421 gagagcgcga gatcgccttc aaagtccggg cctggccgcg ttcgattcgc tttgccagcg
     6481 cgacttcttc ttcccgctcg agcaatcgcc gccgtccggt ctctcggaga tacaaagcga
     6541 cgggatcagc agacacgagc gactcagtcg gcgatagctc ggattctgcg gcgtacgcgg
     6601 gctcttcctg ggtcgctgca gccgaaggga ccacttccgc ttcgtcctcc gcctgcagca
     6661 gatcgatgtc tttttcaatg tcttcgttca cggctgtcat cttctactcc aaaacaaaat
     6721 ctacgcgttc tcgaggtagg tcacccactc gccatagtgt ccgcggctag tcggcgatac
     6781 cacgaccgag cgcatccgat tgacggggaa gggcttagcg catctgcgtc gaggaaggca
     6841 cctactctta ctcgcgcata ttttgatgcc gaaaacgccc aaaacgattc acgtttctga
     6901 cctcgatcca gctatcgtcg ccctgttttc gcgaaccaga ccggacgaat atcccgcagg
     6961 aagatccgcg ccgttacggg gccaacgtgc ttgaattctt gcagttttct cgaaagttcc
     7021 gaagttgtgc gcgagctgga caacagcttt gttactgtcc cgtagcgacg cctgagctcc
     7081 tggcaaacct caagcagttt tgttgctgtc gagaagtcgt agcggacgta gtgcgcgcgg
     7141 tcgagcagcc gtactagttt gtcccatcca gcgcgcatga ttgcatcagc gctcgtcagg
     7201 ccagcgccga ccagttcgcg gtaagcttgc tcggcaatct cggtttgaat cggctttccg
     7261 aacagcaaac acgcgaggaa ccatttgaat agctcctctt ccctccgcga cgcgagattg
     7321 atccgcaggc tgcgcgaata ggattgacgc ccgccgatct tgcgcacccc agcgggccgc
     7381 aagcaaatgg cctctcttcc ccgcttttct gcaagcgcca tccttccaac tcccaagagg
     7441 gcttgtagcg cattcgtgcg aaagaagaag tgatgcccgt cacgggcgtc gacggatcga
     7501 ttgcttcagc ttcgtgccaa tgaaccggag aaacggggcc gttcgagttc ggggcaaact
     7561 cttgttaaca tattcacata catccctgga ggattgcatg caaagcgagc caattatgga
     7621 tttggcccat ctgggccacc tggaattact caccccgaag cccgacgaaa ccctgaaatt
     7681 ctttgtcaac gtgatgggga tgacagaatc gggccgcgaa ggcgagtccg tttatttgcg
     7741 cgcatatgac gactacgagc ggcattcgct gaagctcacg gcctcaaaat tgcccggcat
     7801 gggacacgtg gcgtatcgcg cgcggagccc tcaggcgctc gagcggcgcg tcgcagcgct
     7861 caggggttcc ggctttgaat tcggctggac agatggcgac ctcgcgcacg gtcctgcctt
     7921 tgtatgcaaa gatccggatg gccacaagat cgagctctac tacgagacgc agtggtatga
     7981 agcgccgccc gaactgcgac ccgcgatgaa gaaccaggcg cagaaatatc ccgcgcgtgg
     8041 aatgaacgtg cgccggctcg accactggaa cgggttagcc gtcgacatcg aagcgaaccg
     8101 cgcgtttttc gaaaaatatc ttggcttccg gctgaccgag caaatcgtcc tgaatgacgg
     8161 ctcggaagcc gcgatgtgga tgacctgcac gaacaagagc tatgacttcg cctacacgcg
     8221 cgatcacacc ggaacgaagg gccggtttca ccacatcacc tatgcggtga atagccgcga
     8281 agaagtgctg atcgcggcgg atatcgcgct ggaaaacggt gtctacatcg agacgggccc
     8341 gcacaagcac gcgatccagc agacattctt cctatatttg tacgaaccag gagggaatcg
     8401 tgtggaagtc gccaacgcaa ctgcgcgcct gattctggcg cccgactgga aaccgattcg
     8461 ctggagcgaa gccgagcgta agaaaggtca agcctggggg ctcaagacga tcgaatcgtt
     8521 ccacacctat ggcacgccgc ccgtgtgatg gtttaccgga catgccagaa ttgcggcgcg
     8581 aattcgataa gatccgcgcc gcaatcccgc tgagccacta atcccccgct gatttcgact
     8641 gtttcatcgc ctgctgcatc tgcaccatca tttgccgcat cgcttcaccc gaaccgggcg
     8701 cacgcttttc gcgccaagcc tcgaaacggt ttacgtgctt ttccagattg gcgtattgat
     8761 gtggattctt gcttctctcg cgcatcgcag gcaccaaagc tttgatgcgt ccccagacgg
     8821 cccattgctc tccggtattc tcgaagaaaa agtcctcatc gatcagtccg cggttcacca
     8881 tgctggccgc catttcccaa tagctgatca ccatacgcac agacgcgttt cccgcgctgc
     8941 ccggcggcgc ctgcttgttc atgtcctcaa gcgaggttgg attgaagctc gcgaaatacc
     9001 actcgcgggc ctcgcgcatg cgaggttcgc gccgcagctc gtagagccgg agcaacaggt
     9061 ttgcctgatc gtgcgtcact tctcccatcg tttcctccat tggaatggtc ggatgaaaat
     9121 ccgccggtgc tttacgtctg aaaactatga tgactgtgca gccgccacgg cgccgtgcag
     9181 ctcgacctgg tgcgcctggc gcagcgggat gcgcgattcc tcgaaaccga gcttgatgcg
     9241 ccgccgcagc tcgcgtgcca cgtcgtcctt gcgattcaga gcggttcgaa cctgcagtcg
     9301 caagactgtg ccctccaggg acagcgattc tacgccgagc actcgcgggc cgtcgagcag
     9361 cgcgggagac caatcggcgt cgttgcggaa gtcgccggcg attttttcga gcgtagcaag
     9421 cgcgcgcccg actagctcgt ccgacggaac agtcacatcc acgaatgcct gggaccagtc
     9481 gcggctcagg ttcgcgacct gcccgaccag agagttgggg atcgtcacga tcgcgccact
     9541 gacgttgcgc agaaccgttc gacgcagtgt gagatgttcg acgcgccccg tttcgccgtt
     9601 gagttgaatg aggtcaccca cgacgtactg atcttccaga acgatgaaaa atccattgat
     9661 gagatccttt acaagattct gcgcgccgaa tccgaatgcc aagctgccta cagccgcagc
     9721 cgcttcgaac ggcgtgacgt cgaagccgaa ctccgggagc accatcaacg tggctacagc
     9781 gacgatgatc gcaacgccta cgctgtagag caggccggcg agagtgcggg tctgctgctc
     9841 gcgcatccgt gctccacgag cctgcacctt cgcgatgtcg atcacgcgcg tcgtcagcgc
     9901 cttcaggatg cgtgtcagaa cgaatgcgat gactgcgatg accagaatgc gcgcgccacg
     9961 cgtgccggcg atctccatcc aatgtgcgag cgaacttgtc atggaatatt gggagcgcga
    10021 ttataccatt cacgagacgg cagcccttac agcggcgcga ggccagcact ggaaaagcgg
    10081 cgatgcacgt cttggctttg ctcggcggtg cgctgcaagg tagaatcgag ctattcgttc
    10141 gtcccagcgc gcccgtagct cagttggata gagcgtgtgc ctccggagca caaggtcaga
    10201 ggttcgaatc ctcccgggcg cgccactgca acttcagatt ctaccgatgc aaagtcttcg
    10261 cgattcagta ctcgggtgct ctgaacgagt tttgcgagcg tttttcgcag gccgtgtcag
    10321 ttacctgtgc caaatagcag tatctcctgc gggctgaaga gagcgttgtc tctgatcgaa
    10381 agcctgccgc tgcgctgacc cttcgactgc ggcgtaaacg tgatgtaaat gatgcagttc
    10441 gccgacggaa gcacggtgct gccgtccgtg cacgtggtac cggaagcgat cgcaaaatcg
    10501 ccgaagatat cgacgtcctt cagattcagc ggggtagtgc ctgtattggt aagcgtcacg
    10561 gatttagcgg cgctggtggt gttcgccttc tgggtgccga atgtcaacaa atgcacgttc
    10621 agcgaagcct gtggattgat gatcgttgcc gtcatcggca cggactgcgg gcttcctgcg
    10681 gctgagtcgg taatgataag cgttgcagat tgggtgtcgc tttgatgcgg tgccgggaaa
    10741 aacgtcacga aaattatgca gctccgtcct gcggacagcg ggaaattgca gaacgggctg
    10801 aacgcgaaga attcgttcag gttgccttgt gaaattgcaa tgttccggat gttgatggtg
    10861 ctggtgcccg tgttggtgag cgtaatgctc tgcactccga atgccccgaa gttgacgttg
    10921 ccgaagtcta cggacgtggg cgatatcttg agcgtgctcg tcgaggcggt caccgtctgt
    10981 tgcacgctca aggaagtgct ggtggagaag ttggtgtcgc cggaatagac ggcagtgagc
    11041 atgggcgatt ccaccgtctg gaacgtgatc tggcagcttc ccgctccggc ggtgagcgaa
    11101 gttaccgtgc agctctctcc ggtcgaagca ctcacctgca cgctgcctgt gggtgtgccg
    11161 ttgcccgtga ccgacacgct gatcgtcacc gcctgtcccg tcaccgacgg attcggcgtg
    11221 ttcgaagtga tcgtggtcgt ggtcgatgcc ttctccgcag tgacggatgc cgcttgctgc
    11281 ggagccggcg agtagtcggc attgccggcc tgattggcag ccagctggca ggttcccgtg
    11341 ccgctgatca tggtcacggt cgtgccgttg atcgagcagg cccccgatgc cgtgatcgtc
    11401 accgccaggc ccgacgtggc cgttgctgaa accgggaagc ttgagttgta caccgccgtt
    11461 tccggagctc cggtgaaggc gatcgtctga gcctgttgtg tgaccgtcag cgtcgtgctg
    11521 gtatcggtag ctggggcgaa gtcggtgtca ccggcatagc tgtaggtgat ggtgtagggg
    11581 gtccccgaag gtggaatcgc cgccgtcggg aaggtgagtg agaacacacc gttggcgccg
    11641 agtgtggtgc tctgcactga tccgttgatc gtcaccgaga cggtctcacc gctggatggg
    11701 tacgtcggcc caggcgccga aagcgttccc gtcaggttga cactagaagt gccgaaggga
    11761 atcgactgcg aagccgtcag cccgctgaac gatgtcgcaa gagcctcgac cgtgactccc
    11821 gcagccgaga gcacctgctg cgtgggttgt tcgttgacca cactggccac cgtggccacg
    11881 ttcacatagg agcctgccgc agccgatgcg ccgctgacgt gcagcgtcgc gctggcattg
    11941 gccgcaaggt ctccgaacgt gcaggccagc acctggcttc ccgcggatcc tgtgattccg
    12001 cagcttcccg gacccgtgta ggtgctgagc gaccaggagg cgcccgccgg cagcggatcg
    12061 ttcagcgtga cgtcgggttc cgttcccggg ccgatgttgg tgaccgtgat ggtgaacccg
    12121 atcgtgctcc cggcattcac actggcggcg tcgggagtcg aggtgacgct cagctgtccc
    12181 gaggtggtcg gcgggaaggc caccaccaca tcgctcagat gcttggtcgt gcctccgatg
    12241 ccgggatcga ccggttcgct cgaatcgaag aacgtagtga tgtcgaactc gaactggcag
    12301 gagtagttct gcgccactcc gccaatggtc atcagctcgg tgcagacggt gccgatggca
    12361 tccgaggggc tctgagccga gtcgggatct tcatacaggc gcggcgaact gccggtccag
    12421 tagggagccg gaggagtgaa cgttccgttg ttgaaggtga tcttccagtt caccggaccg
    12481 gagtaggacc cgggatcggg cacgttgccc ggagtaccga catacacatc gtagtggacg
    12541 cagttgccgt tggcgtaggg gtagcagaac ggcgtgatgg tgttgccgtt gggatcggtg
    12601 ccgtagccaa agaaagtgct aaagcggcag tcgaagtcgt tgagcacggt gttgcctact
    12661 tcgcagagtc cgtcgaactg ggagggagga acttcggtcg cggtgacggt gaggttgaac
    12721 gaggagttga cctgggagag ctgcagctgg aaggcatgag cattggcgtc ggtgcaggga
    12781 ttacccggag tgggagtggg attcgagggg cagtcaaacg atgcctgctg agagacattc
    12841 gaccctgcat tgaagggcag atcgaccgac actggcggcg ggatgaagtt agccgtcaca
    12901 ttcaacgccg agctcaccgt caagttgcaa gaagttcctg ttcccgacga gaagcaggca
    12961 ttgccccagc cgccgaaggt ggaaggagaa gtggcggact cattcaaact gatctgtgtg
    13021 ccgctggcgt agctcgccga gcacgtgccg gtcaccacgc cattcgactc gctgcagttg
    13081 atcccgccca ggttgtccgt caccgagccg gtgccggtcc cgatctcggt gatcgtcact
    13141 gcatagttcg gaatcactgg gccgaaagta gcctgctcgg tctgtgcgct gttcatcgtg
    13201 atactgcacg gccccgttcc accgcacgtg ctccagccgt tgaaggctgt gccaacggcc
    13261 ggagttgccg tcagaacgac gacggtgccg ctctgatagc tggctgaaca cgtacccgtc
    13321 acaacgcccg ctgtatcgac gcaattgatt tgaccgagat cgtccgtcac ggtgccgctg
    13381 ccggagccgg gttctgcgat cgtcagcgtg taggaagtcg ggctcgccga taccacacta
    13441 agtacgaccg aagttgagtt gtaagcgacg ctccacgcga ggcccgatgg cagcgctggc
    13501 gagttgatcg acgaaaactg tcccgcgatc gcagatgccg tcaaaatcgt gaatgaattt
    13561 cccaatgagg gcgcaaaccc tccgatgagc cggacactga gcgcacccgc gaggctggcg
    13621 gctcctgcga ctttcacttg cgagtattga gttccaaccg tcgttccgcc gatcgcaact
    13681 tcaaacgatc cggtcgaatt ttgagtgtac gaactcggaa tgttcagcac gcccggtgac
    13741 ggaacgtcac ctgcttcgat gacgcccgcg ttcgtcgtgt tcgaggacag agagcctgta
    13801 ccggaaatca caccggtacc agaggtgctc accccgctag aaacgctcaa cgtgccgtcg
    13861 acttgagcga tgccgccgct gattgaaaat gtcgtgacgg agtccgacac gcccgaagga
    13921 atctggatga agccagctgt ctgcatatac gcggggatcg ttaagccgcc cggtcctgag
    13981 attgagtttc cggaattcgt gagagacgaa ccagcggtgt cctggcggat gtagggagag
    14041 ttgccgttgc cgccgattga cataaccaca gtgccgccgc cggtgagtgt tactgccccg
    14101 ctcatactta agaacgtcgg gttcccattg gctgcgacct ggaacgagcc ggtgttattg
    14161 atcgtcccca gaatc
//
LOCUS       DASPUL010000362         5832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1163053, whole genome shotgun sequence.
ACCESSION   DASPUL010000362 DASPUL010000000
VERSION     DASPUL010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163053"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VEJ00_08780"
     CDS             complement(<1..815)
                     /locus_tag="VEJ00_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXX01293.1"
                     /translation="MALPQKNIRISRFGVFEVDLNAGELRKSGVRLRLQGQPFQVLAL
                     LLERAGEVVTREELQQKLWPSDTFVDFDHSLNTAINKVREALGDSASSPRYVETLARR
                     GYRFIAPVQNEPAMNAINPAAPRAAAAEAGSHRPTLHPELEVPVPHRGITRGLFGLIQ
                     VMYLAFYVAALFRIHAIQSIADSFLPPWGATALAAAVMVTAGVGIPLRCYLISAVGFD
                     YQRLGEKFRQMFPFVLALDQLWAVAPFLLMQKIGLGAAFAASAALLYLPFSERT"
     gene            968..1135
                     /locus_tag="VEJ00_08785"
     CDS             968..1135
                     /locus_tag="VEJ00_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01294.1"
                     /translation="MPSLPREKAREDESSHRHHRHAVYASETTGLLLIAFLLLALTLI
                     RYWHGIHWSLR"
     gene            1138..2100
                     /gene="miaA"
                     /locus_tag="VEJ00_08790"
     CDS             1138..2100
                     /gene="miaA"
                     /locus_tag="VEJ00_08790"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXX01295.1"
                     /translation="MSSEHQPTPQADPLLVVVLGPTASGKTALSLALAQKFDGEIVNC
                     DSVAMYREFDIGTAKPNAAERALVPHHLFNSVGPTEMMTAGEYSRQARRVLCDIRDRN
                     HLPIVVGGTGLYLRALLEGLFPGPQRSEELRERVREREADRGSGYLHRILVRLDRAAA
                     QKIHANDAPKLIRAIEVCLASRQRMTDLWQKGRDPLPGFRILRLGLDPDRAALYNRIN
                     ERARQMFDTGLLDEAGLLLQKYGEDAGPLASLGYKQAVQLLRGELTREQAVQSAQQAH
                     RNYAKRQMTWFRREPEVSWLKGFGDDPQIQSEALARVEISFSGH"
     gene            2161..4035
                     /locus_tag="VEJ00_08795"
     CDS             2161..4035
                     /locus_tag="VEJ00_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HXX01296.1"
                     /translation="MRRILSAILLVLTLSLCAFAKEKDKHSRPLPIQLTRDREKWAEK
                     TLRKLSVEEKVGQVFMIWCRASFLNVENPEYLQLRDAMQKYHVGSFAMTVHVDGPFLL
                     RSEPYEAAELLNRMQRDSRLPLLIAADFERGVSTRLMGATNFPHAMAFGADSKTEDAE
                     TFGRITAEEARAVGVHWNFFPDADVNSNPANPIINTRSFGEDPKQVGDLVAAYIKGAH
                     EGGMLTTVKHFPGHGDTATDSHLGVASVNGDRAHLESIELPPFKQAIAAGVDSVMVAH
                     VTVPTLDSDPNHVATISSAVVSDLLEKQLRFKGLIVTDALDMAGLTHLFANDIGRAAV
                     EAFKAGNDLLLIPADLGASYEAMLRAVQSGEISRERLDRSVLKMLKMKASIGLNQSRT
                     VDLGTIASLVGKPENLQFGQQVADAAITLVRDNGKVLPLKSKGTTKAGLPYMTREETH
                     NDTVALLFSDDVRTDSGRAFGREFRVRIPDARVIYVDPRVAAGMSDEVLKAVDEARTV
                     VAAVYVIPTAGKVGNSVAMADATGTLLQQLLDHAAAKTVVVAMGNPYLASDFPKIENY
                     MCTFSNATVSEVAAVKALYGEISTHGHLPVTIPNVAQRGAGLEHPAHVANGGFPHAQN
                     "
     gene            4022..4606
                     /locus_tag="VEJ00_08800"
     CDS             4022..4606
                     /locus_tag="VEJ00_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01297.1"
                     /translation="MRRTRLLTAVALAGVAAALLLPACSVNVKKEKNGEDKQVDISTP
                     VGGIHVSKNANAGDVGLSIYPGARLKQKDSSGDDKSANVNISGFGYGLKVVAMEYQSD
                     DPPSKVVTFYQDQLKKYGKVLECHTSHLDVDADIKESDHDSHELTCQESSGQTIELKV
                     GTKENQHIVAVEPDGKGSSFSLVFVRTHGKEADI"
     gene            4802..5470
                     /locus_tag="VEJ00_08805"
     CDS             4802..5470
                     /locus_tag="VEJ00_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXX01298.1"
                     /translation="MEQPCYKCGQSVEEGTPFCPHCSAPQIRVVVTDAVATPLICAAG
                     AASTDSPALSSPHPLSAPALPTHWADTLKPGALAALVAALLMMLGLNPFVAMISAGFL
                     AVVFYRHRQPGAPITPRVGSRLGAVSGLLWFGLSVALQGTLVLLLHKGPELRDEALKR
                     IQQTAPNTSDPQVQAWFEYFKTPAGLTVLLVLAVIFAFVAALILGVLGGALAGAVLSR
                     REKG"
     gene            5550..>5832
                     /locus_tag="VEJ00_08810"
     CDS             5550..>5832
                     /locus_tag="VEJ00_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01299.1"
                     /translation="MPARGSLLGIEHLEAKEILGLLKLARRMNPQKPRPLLRGKRIVL
                     LFYEASTRTRSSFEIAAKSLGAMTTLVQSSGSSIEKGESIIDTGYTLRAV"
BASE COUNT         1165 a         1787 c         1734 g         1146 t
ORIGIN      
        1 gtccgctcag aaaacggcag gtaaagcaga gcggcgctcg cggcaaaggc cgctcccagg
       61 cctatcttct gcatcaacag gaatggcgcc accgcccata gctggtcgag cgccaggaca
      121 aacggaaaca tctgccggaa tttttcgccc agccgctggt aatcgaagcc caccgcagag
      181 atcaggtagc agcgcagcgg tattcccacc cctgccgtca ccataaccgc cgcggcgagg
      241 gcggtcgcac cccatggcgg aaggaaggaa tctgcaatgc tctggatggc atggattcgg
      301 aacagggcgg cgacataaaa ggccaggtac atcacctgga taagcccgaa caacccgcgt
      361 gtaataccgc ggtgcgggac gggaacttcg agttcaggat gcagggtcgg gcgatgagat
      421 ccggcttccg cagctgcggc ccggggcgct gccgggttga tggcgttcat cgccggctcg
      481 ttctgcacgg gcgcaatgaa ccggtatccg cggcgggcga gggtctcgac ataacgcggg
      541 ctggatgccg aatctcccag ggcctcccgg accttgttga tcgcggtatt cagactgtga
      601 tcgaagtcga cgaaagtgtc ggagggccaa agcttctgct gcagctcttc gcgcgtgact
      661 acttccccgg cgcgctccag caacagcgcc agcacctgga acggctgacc ttgcaaccgc
      721 agccgcaccc cgctcttgcg gagctcgccc gcattgagat cgacctcaaa cacaccaaag
      781 cggctgattc gaatgttttt ttgcggcagg gccatggacg acgccgtagt gtagcaccgg
      841 agccgggcta ctacggctct tgacccgacc gcatagagga gcacggatga atccctcttg
      901 ccggccggtg catccttctg caccttctgc agttaaaggg tcttgcgcgc agcgctacaa
      961 tatgcgcgtg ccctccctgc cacgcgagaa agcgcgcgaa gacgagtctt cgcaccggca
     1021 tcatcgccat gccgtctacg cttcggaaac cacaggcctg ctcctgatcg cgtttctgtt
     1081 gctggccctt accctgattc gttactggca cgggattcac tggagcctgc gctgaacgtg
     1141 agttcggaac atcagccgac tccgcaagcc gacccgcttc tggtggttgt cctgggcccg
     1201 actgccagcg gaaagacggc cctgtcgttg gctctcgcgc agaaatttga cggcgagatc
     1261 gtcaactgcg actcggtggc catgtatcgc gagtttgaca tcggcacggc caagcccaac
     1321 gccgcggagc gcgcccttgt tccccaccat cttttcaatt cagtcggtcc gaccgagatg
     1381 atgactgccg gtgaatattc ccggcaagcg aggcgggtgc tgtgtgacat tcgcgaccgc
     1441 aaccatttgc cgatcgttgt cgggggcacc ggattgtacc tgcgcgccct gctggaggga
     1501 cttttcccgg gcccgcaacg ctcggaagaa ctgcgcgagc gagtgcgcga gcgcgaagcg
     1561 gatcggggat caggatatct ccaccggatt ctcgtgcgcc tcgatcgcgc ggctgcgcag
     1621 aagattcacg ccaacgacgc tccgaagttg atccgcgcca tcgaggtctg cctggcgtcg
     1681 cggcagagga tgaccgactt gtggcagaag gggcgtgatc cgctgccggg ctttcggatt
     1741 ctcaggctcg gcctcgatcc cgaccgcgcc gcgttataca accgcattaa cgaacgcgcg
     1801 cgtcaaatgt tcgacaccgg tctgcttgac gaggccgggc tcctgctgca gaagtacggc
     1861 gaagacgcgg gtccgctggc atcgctggga tacaagcaag cagtacagtt gcttcgcggc
     1921 gaactcactc gcgagcaggc cgtgcaatct gcgcagcagg ctcaccgcaa ctatgccaaa
     1981 cgccagatga cctggttccg ccgggagccg gaagtcagtt ggcttaaagg tttcggcgac
     2041 gatccacaga ttcagagcga ggcgctggca cgagtcgaga tttctttctc cgggcactga
     2101 cagacataac tccacttttg agtcgtcagc acagccggga aactctgcta tcgttccccc
     2161 atgagacgaa tcctgtctgc catcttgctg gttctgactc tctccctttg cgcctttgcc
     2221 aaggaaaaag acaagcactc gcgcccgttg ccgattcagc ttacgcgcga ccgtgaaaag
     2281 tgggccgaga agacactccg aaaacttagt gtggaggaaa aggtcgggca ggttttcatg
     2341 atctggtgcc gtgccagctt cctcaacgtg gagaatccgg aatacctgca actgcgcgac
     2401 gcgatgcaga aataccacgt cggctccttc gccatgacag tgcacgtcga cgggcccttt
     2461 cttcttcgga gcgagcccta cgaagcagcg gaattgctga accggatgca acgtgactcc
     2521 aggctgccgc tgctgatcgc tgcggacttt gagcgcggtg tttcgacgcg gctcatgggg
     2581 gcgacgaact ttccccacgc catggccttc ggcgccgata gcaagaccga agatgccgag
     2641 acgttcggcc gcattaccgc ggaagaggcc cgcgcggtgg gggttcactg gaatttcttt
     2701 cccgatgccg acgtcaactc gaacccagcc aatcccatca tcaatacgcg gtcattcgga
     2761 gaagacccga aacaggtcgg cgatctcgtc gcagcctata tcaagggagc gcatgaaggt
     2821 ggaatgttaa cgacggttaa gcacttccct ggccacggcg ataccgccac cgactcccat
     2881 ctcggcgtgg ccagcgtcaa tggcgatcgc gcccatctgg agtcgattga acttccgccg
     2941 ttcaagcagg ccattgcggc gggcgtcgac tcggtcatgg tggcgcatgt cacggttccg
     3001 actctggatt ccgaccccaa ccacgtcgcg accatctctt ccgccgtcgt ttcggacttg
     3061 ctggagaaac aactgcggtt caaagggctg atcgtgaccg acgctctcga catggcaggt
     3121 ctgacccatc ttttcgcgaa tgacatcggc cgcgccgcgg tggaggcctt taaggccggc
     3181 aacgacctgc tgctgattcc cgccgacctg ggcgcatcct acgaagccat gctaagagct
     3241 gttcagtcag gcgagatctc ccgcgagcgg ctcgaccgct cggtgctcaa gatgctgaaa
     3301 atgaaggcgt ctatagggct gaaccaatcg cgcacggttg atctaggaac gattgcgagc
     3361 ttggtcggaa aaccggagaa tctccagttc ggccagcagg tcgccgatgc cgccatcacc
     3421 ctggtgcgcg acaacggcaa agtgctccct ctcaaatcca agggaaccac gaaggccggc
     3481 cttccgtaca tgaccaggga ggagactcac aacgatacgg tggcgctcct cttttcggac
     3541 gacgtgcgca cggattccgg ccgtgctttt ggacgcgaat ttcgtgtccg cattccggat
     3601 gcgcgtgtga tttacgtcga tccccgtgtc gctgccggaa tgagcgacga ggttctcaag
     3661 gctgttgacg aagccaggac tgtggtggct gcggtttacg tcattccgac cgccggcaag
     3721 gtcggcaatt cggtggcgat ggcagatgca acgggcacgc tgctgcagca gttgctcgat
     3781 catgccgcgg cgaagacagt agtggtggcg atggggaatc cgtacctggc gagcgatttc
     3841 cctaagatcg agaattacat gtgtaccttt tccaacgcga cagtttcgga agttgcagcg
     3901 gtcaaggcgc tgtatggtga gatttcgacc cacggccact tgccggtcac gattccgaac
     3961 gtcgcccagc gtggcgccgg cctcgagcac ccggcgcacg tcgccaatgg agggtttccc
     4021 catgcgcaga actagattgc tgactgcagt tgcgctcgcg ggtgttgcag ccgcgctgct
     4081 gcttcccgct tgcagcgtaa atgtcaagaa agaaaagaac ggggaagaca aacaggtcga
     4141 catcagcacg ccggtcggcg gcattcacgt gagtaagaat gccaatgccg gcgatgtcgg
     4201 tttgtccatc tatcctggcg cccgtttgaa gcagaaagac tccagcggcg atgacaagag
     4261 cgcgaacgtc aacatctctg gattcggcta tggcttgaaa gtcgtagcca tggagtatca
     4321 atccgacgat cctccttcca aggtggtcac cttctaccag gaccagctca aaaagtacgg
     4381 caaagtcctt gaatgccaca ccagccatct ggacgtggat gccgacatca aggaatctga
     4441 ccacgattcg catgagctga cctgccagga gtccagcggc cagaccatcg agctgaaggt
     4501 agggaccaag gagaaccagc acattgttgc cgtcgaaccc gatgggaaag gttcgagctt
     4561 ctcgctggtt ttcgtgcgaa cccacggcaa ggaagctgac atctaggcaa ggatctccgc
     4621 cgctagtcgt ttgcgttcct tgcagagcat ttctaccttt cgccagtggc tttgagcaca
     4681 taacgaagac tccggccaga accgcgtgtg cgttgttcac gaaggcatcc ttgcctctgc
     4741 ccctccggct gcggtacttt cagaaccaag cccttcttcg acagctcgcg atcgaacgga
     4801 aatggaacaa ccctgttaca agtgcggtca aagcgttgaa gagggcacgc ctttctgtcc
     4861 gcattgctct gccccgcaga ttcgcgtggt tgtgaccgac gcggtcgcaa ctcctctgat
     4921 ctgtgccgcg ggcgccgctt ccaccgattc gcccgctctt tccagcccgc acccgctctc
     4981 cgccccggca cttccaacgc actgggcaga cacgctaaag ccaggcgcac tggccgccct
     5041 ggtcgcagcg ttgctgatga tgttgggact gaatccgttc gtggccatga ttagcgccgg
     5101 ctttcttgcg gtggttttct accgtcatcg tcagcctgga gcaccgatca cgcctcgagt
     5161 tggttcccgg ctgggcgcgg tcagcggact cctgtggttt ggtctgtcgg tggccttgca
     5221 gggcacgctc gtgctccttc tgcacaaagg cccggagcta cgggatgaag cgcttaagag
     5281 gattcagcaa accgccccaa ataccagcga cccgcaggta caggcatggt tcgagtactt
     5341 caagacgcca gctggcctca cggtattgct cgtccttgcg gtaatttttg cattcgtagc
     5401 ggcgctcata ctgggtgttt tgggtggcgc cctggccgga gccgtcttga gccgccgcga
     5461 gaaaggctag tccgagagcg ggaacggcga cccttgcagc cctccttgct cgccgcgtat
     5521 catgcggcgt tcaccttcaa gcgatgaata tgcccgctcg cggttcgctg ctcgggatcg
     5581 aacacctcga ggcgaaggag atcctcggcc tgctcaagct ggcccgccgc atgaatccgc
     5641 agaagccgcg tccgctgctg cgcggcaagc gcatcgtgct tctgttttac gaggcctcca
     5701 cacgcacccg gtcttcattt gaaattgcgg cgaaatctct gggcgcgatg accacgctgg
     5761 tgcagtcctc cggatcgagc attgaaaaag gcgaatccat catcgacacg ggatacaccc
     5821 tgcgcgcggt gg
//
LOCUS       DASPUL010000363         3264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1984055, whole genome shotgun sequence.
ACCESSION   DASPUL010000363 DASPUL010000000
VERSION     DASPUL010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1984055"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2729)
                     /locus_tag="VEJ00_08815"
     CDS             complement(<1..2729)
                     /locus_tag="VEJ00_08815"
                     /inference="COORDINATES: protein motif:HMM:NF026036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXX01300.1"
                     /translation="MLLVRRLAVVIILLSPTFALAQQPQEGRLMRFPDVRGDKVAFVY
                     GGDIWVASTSGGTARRVTTHAGRELFPKFSPDGKWLAFTGQYDGNFNVYVMSAEGGQP
                     RQLTFYQGAAQPLNDRMGIHSEVINWMPDSKRVVFLSRRDASNGWTKRPFTVSIDGGQ
                     PEPLPMDQGGLLSFNADGTKIAYNRIFRNFRTWKRYTGGLAQDIYIFDIKNNVFEQKI
                     PHTDYTDTFPMWHGNTVYFTSDRGPEHRLNLYSFDLGSKQVEQLTHFADFDVMWPSLG
                     DASIIFENGGYLYLFDLSSKQATKLTIMLPGERDLMMKHWAGVSKLITDFDIAPDGKR
                     AVFAARGDVFTVPAKDGATRNLTRTPGIREKEVAWSPDGRWIAYVSDKTGEDEIYITP
                     QDGMGNIELGKEPEPKDKEKDKEAAAKEAAREKERGKDKEQQITSGYKGFKFAPAWSP
                     DSKRLAWSDKDLRLWFVDIADKKPVEVDRGRYNEITNYNWSPDSKWIAYDKQLASGVS
                     VVYLYSTADKKITPVTNDLVNSNSAVFDPEGKYLYFLSDRDYNEVLGNLDFEFANPKT
                     TRPYLVTLQADAPSPFPALSDETAIKREEPAPAASESEKDKKKEEKKKTEEKKEEKPG
                     EKEAAEIAKNFRIDLDGIQDRIVSLPVPPASITELNAAKGFLYYSTQPVQGLSGPLPG
                     EETAIHAYDMKERKEKTVIEGGQRWALSFDGTKILYQTKDGAYGIIDAKSDGPKKAGE
                     GALNLSGMRAEIDPPAEWQQIFNEVWRQERDYFFEASMNGVDWEAVRKKYEPLVAYAA
                     NRYDLTYILGEVIGELSNSHTYVGGGDQPDLHPINVGLLGVDYELDAASGMYRFKKIY
                     PGENWTPDTRSPLTEPGVNVKEGDYLLAVNGRPLRAPQTPEELLVNTANETTA"
     gene            complement(2919..>3264)
                     /locus_tag="VEJ00_08820"
     CDS             complement(2919..>3264)
                     /locus_tag="VEJ00_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01301.1"
                     /translation="LEVGGTGLWSRWSTARAASKLHTVLLDVNELTERADNAIKFLSD
                     MFSARLYKLCAQKVGVPDYKDLVQEKLHTAEDLYRFMVEQFNQSRAFVLELMVVVILV
                     IELVYFFKGHPL"
BASE COUNT          605 a          941 c          977 g          741 t
ORIGIN      
        1 atcgcggtgg tttcattggc cgtgttgacg agcagttcct ccggagtctg tggggcgcgc
       61 agcggacgtc cgttcacggc gagcaggtag tcgccttcct ttacgttgac acccggctcg
      121 gtgagaggcg aacgcgtgtc cggagtccag ttctcaccgg gatagatctt cttgaagcga
      181 tacatgccgc tggccgcatc gagctcgtaa tccactccca gcagccccac gttgatgggg
      241 tgcagatcgg gctgatcgcc cccgccgaca taggtatggg agttcgaaag ttccccgatc
      301 acttcgccta gtatgtaggt gaggtcgtag cgattcgcag cgtaagccac caagggctcg
      361 tatttcttgc gaaccgcttc ccagtccaca ccgttcatgc tggcttcaaa aaagtagtcg
      421 cgctcctgcc gccacacttc attgaaaatc tgctgccact ccgccggggg atcaatctca
      481 gcacgcatgc cggagagatt gagcgcgccc tcgccagcct tcttcggccc gtcagatttg
      541 gcgtcgatga tcccgtacgc gccgtccttg gtctggtaga gaatcttcgt gccgtcgaac
      601 gatagcgccc agcgctggcc gccttcgatg acggtcttct ccttgcgctc cttcatgtca
      661 tacgcgtgaa tggccgtttc ttcgccggga agcggcccgg agagcccttg caccggctgg
      721 gtcgagtaat agagaaatcc cttggcagcg ttcagttccg taatgctggc cgggggaacc
      781 ggcagagaca cgatgcggtc ctgaatcccg tcgagatcga tgcgaaagtt cttagcgatc
      841 tcggcagcct ccttctcgcc gggtttctct tccttcttct cttcagtttt tttcttctct
      901 tccttctttt tgtccttctc ggactccgag gcagccggcg ccggctcttc acgcttgatc
      961 gcggtctcgt cgcttaacgc gggaaacggc gagggagcgt cggcctgcag cgtcaccaga
     1021 tagggccgtg ttgttttcgg attggcaaat tcgaagtcga ggttgcccaa aacttcgttg
     1081 tagtcgcgat ccgatagaaa gtagagatac tttccctctg gatcgaaaac cgcgctgttg
     1141 ctgttgacca gatcattggt gacgggcgtg attttcttgt ccgctgttga atagagataa
     1201 acgacgctca ctccgctcgc cagttgtttg tcgtaagcga tccacttgct gtcgggcgac
     1261 cagttgtagt tcgtgatctc gttgtacctg ccgcggtcga cttctaccgg cttcttgtcg
     1321 gcaatgtcca caaaccacag ccgcaggtcc ttgtcggacc aggccagccg cttgctgtcg
     1381 ggcgaccacg caggggcaaa cttgaatcct ttgtagccgc tggtgatctg ctgctccttg
     1441 tccttgccgc gttctttctc tcgcgccgct tccttggcgg cggcctcctt gtctttctct
     1501 ttgtccttgg gctcaggctc cttccccagt tcgatgttgc ccatgccgtc ctgcggcgtg
     1561 atgtagatct cgtcttcgcc ggtcttgtcg gaaacatacg cgatccaccg cccatccgga
     1621 gaccacgcca cttctttctc gcgaataccc ggcgtgcgtg tcagattgcg cgtcgcaccg
     1681 tcttttgctg gcacagtgaa cacatcgccg cgcgcggcaa acactgcccg cttgccgtcg
     1741 ggggcaatat caaaatcggt gatcagcttg ctgacgccgg cccagtgctt catcatcaga
     1801 tcgcgttcgc cgggtagcat gatcgtcagc ttcgttgcct gcttcgaact caaatcgaac
     1861 agatagagat acccgccgtt ctcgaagatg atcgatgcat cacccaggct cggccacatc
     1921 acgtcgaaat ccgcgaagtg cgtcagttgc tcgacctgct tcgatcccaa atcaaagctg
     1981 tacagattca gccgatgttc cggcccgcga tccgatgtga agtagacagt gtttccgtgc
     2041 cacatgggaa acgtgtcggt gtaatcagtg tgcggaatct tctgttcgaa gacgttgttc
     2101 ttgatgtcga agatgtagat atcctgcgcc agtccgccgg tgtagcgctt ccaggtgcgg
     2161 aagttgcgga agatgcggtt gtaagcgatc ttggtgccgt cggcgttgaa cgacagcagc
     2221 ccgccctgat ccatgggcag cggttcgggc tgcccgccat cgatggaaac cgtaaacgga
     2281 cgcttggtcc atccgttcga tgcgtcgcgg cgggagagaa acacaacgcg cttgctgtca
     2341 ggcatccagt tgatcacttc actgtgaatc cccatgcgat cgttgagcgg ttgcgccgcg
     2401 ccctggtaaa acgtcagctg ccgcggctgt ccgccctcgg cgctcatgac gtagacatta
     2461 aagttgccgt catactggcc ggtaaaagcc agccactttc cgtcgggtga gaatttagga
     2521 aacagctcgc gcccggcatg cgtggtcacg cgccgtgcgg tgccgccgct ggttgaggcg
     2581 acccagatgt cacctccata aacgaaggcg accttgtcgc cgcggacgtc gggaaagcgc
     2641 atcaggcggc cttcctgcgg ttgctgggcg agagcgaacg tgggagagag aagaatgatt
     2701 acgactgcaa gacggcgaac gagtagcacg gtcagagcct cctaggggga cgtgcggagt
     2761 gccgggcgcg atcaacgtgc gccgggcaaa cgggaaattg taacaggaag gttaagcatg
     2821 gagcgcgcgc gtgggaccaa cagcggcaga cagtttgtga gtgcagggga gtcggacagc
     2881 cgaggagggc tgtccctaca tggatcgtgg aaattccgtt acagcgggtg gcctttgaag
     2941 aaatagacca gttcgatgac cagaatcacg acgaccatca gttcgaggac aaaggcgcgg
     3001 gactggttga actgctcgac catgaagcgg tagaggtctt cggcggtgtg cagcttttcc
     3061 tggacgaggt ccttgtagtc ggggacgcca accttctggg cgcagagctt gtagaggcgc
     3121 gcgctaaaca tgtcgctaag gaacttgatg gcgttgtcgg cacgctcagt gagctcgttg
     3181 acgtcgagca ggacggtgtg aagtttggag gcggcgcggg cggtgctcca gcgcgaccac
     3241 agtccggtgc cgccgacttc cagg
//
LOCUS       DASPUL010000364         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1433625, whole genome shotgun sequence.
ACCESSION   DASPUL010000364 DASPUL010000000
VERSION     DASPUL010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433625"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VEJ00_08825"
     CDS             <1..558
                     /locus_tag="VEJ00_08825"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HXX01302.1"
                     /translation="FFWEHSLGCALVSRHFARKISYPDPGKAYLAGLLHDLGIIVNLW
                     VLPKDFQEAFEVARARGIPLHEAEQEVLGFTHCESGRLLAERWELSPDLTEVVALHHT
                     PTESLNHAGLVGLVELSDLLCRMSGLNYGYIENRQVNLLEQTGFAILSKQCQGLKDLD
                     WARLTFEMDSYMDEVHALVHAIYRS"
     gene            555..1643
                     /locus_tag="VEJ00_08830"
     CDS             555..1643
                     /locus_tag="VEJ00_08830"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HXX01303.1"
                     /translation="MTPDTAASPPIRVLVVDDSAFMRIALARMISSESGFEVVGTATS
                     GRDALTKIPSLDPDVITLDVEMPGLDGLETLRKIMTRWPHPVIMVSATTEKDAETTFV
                     ALGAGAFDYVPKQLSGTSLDILHIQPDLLAKIRAAAAWRRSQRINPSARKPPRSTVIE
                     SVDTGSATPAIVVIGTSTGGPKALQETLPLLPRELPVPVLVVQHMPAGFTSAFAQRLN
                     ALCSVTVREAVDRDVLQPGVVYIAPAGMHMTVERPSESRAFICLDTQPENSLHIPSVD
                     VTMKAVAESFGNLAMGVIMTGMGSDGALGMEAIHHKGGITIGQDEATCTVYGMPRACA
                     ELGVLQRVVPLSQISEQIVKATRYRKRA"
     gene            complement(1640..>2096)
                     /locus_tag="VEJ00_08835"
     CDS             complement(1640..>2096)
                     /locus_tag="VEJ00_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXX01304.1"
                     /translation="AHTLENTVEAGAWIDCSRKQAPAGALTILLVEDEKFVREATRNV
                     LRAVGYRVLTARSAEEAERVYDLQRDHVKLLLTDVVLPGESGISLARRLRRENTGLGV
                     IFVTGYGEKMKLSESEPGECLAKPFSAGVLLQRIRDILAAQTSAEARSA"
BASE COUNT          399 a          700 c          565 g          432 t
ORIGIN      
        1 ttcttctggg agcactctct cggttgcgcc ctggtctccc gccacttcgc ccgcaaaatc
       61 tcgtatcccg atcccggcaa ggcatacttg gccggattgc tccacgatct gggaatcatt
      121 gtgaacctct gggttctgcc caaggatttt caggaggcgt tcgaagtcgc ccgtgcccgc
      181 ggcattcccc tgcacgaggc cgagcaggag gttctggggt ttacgcactg cgaaagcggc
      241 cgtctgctgg ccgagcgctg ggaactctct cccgacctca cggaagtcgt ggcccttcac
      301 cacaccccca ccgagtcact aaaccacgca gggttggtcg ggctggttga actcagcgac
      361 ctcctctgcc gcatgagcgg gctgaactat ggctacatcg aaaaccggca ggtcaacctg
      421 ctggagcaga ccggcttcgc cattctatcc aagcagtgcc agggcctgaa ggacctggac
      481 tgggcccgcc tcacttttga gatggattct tatatggatg aggtgcacgc gctcgtccac
      541 gcgatctatc gatcatgaca ccggacactg ctgccagccc gccaatccgt gtacttgtgg
      601 tggacgacag cgctttcatg cgcatcgcgc tggcccgcat gatctcatcg gaatccggct
      661 tcgaggtcgt cggcaccgcc acgagcggac gggatgcgct gacgaaaatc ccttcgctgg
      721 atcccgatgt gatcactctc gatgtggaga tgcccggcct cgacggcctg gagactttac
      781 gcaagatcat gactcggtgg ccgcaccccg tcatcatggt cagcgctacc accgagaagg
      841 atgcagagac caccttcgtc gcgcttggtg cgggcgcctt tgactatgtt cccaagcagc
      901 tctccggcac atcgctcgac attcttcaca tccagccgga cctgctggcc aagattcgag
      961 ccgccgcagc ctggcgccgt tctcaaagaa tcaatccatc ggctcgaaaa ccacctcgat
     1021 cgaccgtgat cgaatcggtt gacaccggtt ccgccacccc ggccattgtt gtcatcggaa
     1081 cttcgaccgg cggccccaaa gctctgcaag agaccctgcc ccttcttccg cgcgagcttc
     1141 ctgtgccagt cctcgtcgtg caacatatgc cggcgggatt cacgtcagcc ttcgcgcaac
     1201 gtctcaacgc cttgtgttcc gtaacggtgc gcgaagccgt tgaccgcgac gtgcttcagc
     1261 ctggcgtcgt atacatcgca ccagccggga tgcacatgac cgtggaacgt ccctccgagt
     1321 cgcgggcctt catctgcctc gatactcagc ccgagaattc cctgcacatt ccctccgtcg
     1381 acgtcacgat gaaagcagtt gccgaaagtt tcgggaacct ggcgatgggg gtgatcatga
     1441 cgggaatggg ctctgacggc gctctgggga tggaagccat tcaccacaaa ggtggcatca
     1501 ccataggcca ggatgaggcg acctgcaccg tctacggaat gccgagggcc tgcgccgagc
     1561 tgggcgtgct ccaacgtgtg gtcccactgt cgcaaatctc cgagcagatc gtcaaagcaa
     1621 cgcgttaccg caaacgcgcc taggcagatc ttgcttctgc tgacgtttgc gccgcgagaa
     1681 tatccctgat tcgctgcagc agaactcccg cggaaaacgg cttcgccaga cactcccccg
     1741 gctccgattc actgagcttc atcttctcgc cgtagcccgt aacaaaaatg acgccgaggc
     1801 ctgtgttttc ccgcctcagc cttctggcca gcgagattcc actctctccg ggaagcacta
     1861 catccgtcag cagaagcttc acatggtcgc gttgcagatc gtaaacccgc tccgcttcct
     1921 ccgcgctcct tgccgtcaag acccggtatc ctactgcgcg cagaacattc cgggttgcct
     1981 cccgcacaaa cttctcgtct tccaccagca gaattgtcag cgcgccagcc ggcgcttgct
     2041 ttcggctgca atcgatccag gcaccagcct ctacagtatt ttcgagggta tgcgct
//
LOCUS       DASPUL010000365         4079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1985023, whole genome shotgun sequence.
ACCESSION   DASPUL010000365 DASPUL010000000
VERSION     DASPUL010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1985023"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VEJ00_08840"
     CDS             <1..485
                     /locus_tag="VEJ00_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01305.1"
                     /translation="GDERMMEVPAGFGRRLERRLAQNARASSRGWSTWSTWLIPVAAV
                     LLLAGGLRLANSLTSASPLKSEHAQAGQGIPPDMLVVVSTDAKIFHVAGCDFIHNKDK
                     VRTLTAKEAMREGYVPCLRCMRKYLQTAFAGHLTPETEADVKLDGADVEEQKIPGLGR
                     "
     gene            complement(561..836)
                     /locus_tag="VEJ00_08845"
     CDS             complement(561..836)
                     /locus_tag="VEJ00_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide MXKDX repeat protein"
                     /protein_id="HXX01306.1"
                     /translation="MKKILSTLALASFLAASMSAFAQDQMKLDDMKKDDQSSMQNDQM
                     KKDDMSKDQMKKDKKSKKAKKDKMKKDDMKKDDMKKDDMSKDQMKNN"
     gene            complement(980..2257)
                     /locus_tag="VEJ00_08850"
     CDS             complement(980..2257)
                     /locus_tag="VEJ00_08850"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXX01307.1"
                     /translation="MAQEVRNIRRQKLPNGLTIITEQMQHIRSASIGIWLQTGSRDEA
                     PEWNGISHFIEHMVFKGTKHRSAEEIARQVDSIGGNMDAFTAKECICFNVKVLDEHVP
                     TALDILSDLVLHPVFDAADIARERGVIMEEIKMDEDNPDYLVHEIFTQNFWKDHPLGK
                     PILGTKETVKRFEREAVIDAYHHRFAPANMIVSAAGNLDHDRFVELVTGHFQHMKPVK
                     NGFHSSAPKIVSRITLRNKKALEQVQVCMGVPAYPIAHEQRHAGYILNTLLGGGMSSR
                     LFQNIRERQGLAYSIYSDLNPYRDTGCLAVYAGTSLASATKVVQSVVSEFRKLKTEPV
                     SREEVRRAKDQLKGSLMLSLESSTARMSNLARQEMYFDRFYDLDELIEKIEAVTADDL
                     IGLANDFFKTESVAVTALGNLNGLKLTRDQLAC"
     gene            2470..3585
                     /gene="rlmN"
                     /locus_tag="VEJ00_08855"
     CDS             2470..3585
                     /gene="rlmN"
                     /locus_tag="VEJ00_08855"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXX01308.1"
                     /translation="MADPAQIALLGLDRSEIASLIEGMGEPSYRAQQILDAVYRQRLD
                     SIEQISTLPQSLRSRLAEQGIAVGLPQIERRFVSQDGTVRYLMGLVDGQSVETVWMPE
                     GDGGEEGDGTEAGNGEGWERTTICVSSQVGCAVDCQFCLTALLGVKRNLTAGEIAGQV
                     CVVLKDQKVSPPQHRINLVFMGMGEPFLNYDNFMKAVRLLVEETNVAESRMTVSTAGI
                     VPRLYDFGQEPLRPKLAISLNAPNDALRTELMPLNRKWSLENLIMAARDFPLRNRERI
                     TFEYVLLRGINDATENAREVVELLRGLRAKVNLIALNPGPGISFETPAEERVVAFQQI
                     LIKAGIPAFVRRPRGRDIYAACGQLKRTVEIAAAPIL"
     gene            complement(3545..>4079)
                     /locus_tag="VEJ00_08860"
     CDS             complement(3545..>4079)
                     /locus_tag="VEJ00_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01309.1"
                     /translation="LVAGLISFAQQAPAPQTPTDLNTLARTAVKLTQPQWQALNIEVR
                     AEFDPALPKVLGDSNQLLQVCLQLLGNCLHLMSERGSRLLTVNTENCDGVCVLQIATD
                     PPASLARGDAQTAPDATDGLALSACEGILKEHHGHICCESRSDGAMNVRVDLPAASAS
                     GIHRATVSALLQSQPYA"
BASE COUNT          806 a         1104 c         1250 g          919 t
ORIGIN      
        1 acggcgatga gcgcatgatg gaagtgccgg cgggatttgg ccggcgtctg gaacggcgac
       61 tggctcagaa tgctcgcgcc agcagcaggg gctggtctac gtggtcgacg tggctgattc
      121 ccgtggcggc ggtattgctg ttagcgggcg gattgcgcct ggccaattct ctgacgtccg
      181 cgagcccgct gaagtccgaa catgcgcagg cagggcaggg cattccgcct gacatgctgg
      241 tcgtcgtctc tacggatgcc aagatctttc acgtggccgg atgcgacttc attcacaaca
      301 aggacaaggt ccgaaccctc actgccaaag aggccatgcg ggagggctac gtgccgtgcc
      361 tgcgctgcat gaggaaatat ctgcaaacgg cgtttgccgg gcacttaacg ccggagactg
      421 aggccgacgt gaagctggat ggggcggacg tggaagaaca gaagattccc ggcttaggaa
      481 ggtaaacgat caaggggaat catgcggcaa aaagggccct gctcgagggc cctcgttttt
      541 gcaaaggtag aagcccccgc ctagttattc ttcatctggt ccttgctcat gtcgtctttc
      601 ttcatatcgt ctttcttcat gtcgtccttt ttcatcttgt ctttcttcgc cttcttgctc
      661 ttcttgtcct tcttcatctg atccttgctc atgtcatctt tcttcatctg gtcgttttgc
      721 atggagctct ggtcgtcttt cttcatgtcg tctagcttca tctgatcttg cgcgaaggcc
      781 gacatcgaag cagccaggaa agaggcgagt gcgagagtgc tgagaatctt cttcatggtt
      841 tctccgtgga tgaggtttgg tcctgcggca ggttcttgcc tgcaggtgga gaagggatgc
      901 acgactgcgg tggaagttac gggaggagag acctatctat ggtgttatgt gcagacaaca
      961 gttcaacgcc tgtgcaaaat cagcaagcca gctgatcgcg ggtaagtttt aatccgttca
     1021 gatttccgag cgcggttacg gcgacggatt cggtcttgaa aaagtcgttc gccagaccga
     1081 tgagatcgtc cgccgtcacg gcttcaatct tctcgatcaa ttcatccagg tcgtagaagc
     1141 ggtcgaaata catttcctgg cgggcgaggt tcgacatgcg tgcggtgctt gactcgagcg
     1201 agagcatcag gctgcctttc agctgatcct tggcgcgccg cacttcttcc cttgaaaccg
     1261 gttcggtttt cagtttccgg aattcgctga ccaccgactg cactaccttg gtagctgagg
     1321 cgagtgatgt ccccgcatag acagccaggc atcctgtgtc gcgataagga ttgaggtcgc
     1381 tgtaaatcga atacgccagg ccctggcgct cgcgaatgtt ctgaaacagg cgcgagctca
     1441 tccctccgcc gagcagcgta ttgagaatgt atcccgcatg ccgttgctcg tgggcgatag
     1501 gatacgcggg cacgcccatg cacacctgca cctgttcgag agccttcttg ttgcgcagag
     1561 tgatgcggga gacgatcttg ggcgcagagg aatgaaaccc gttctttacc ggcttcatgt
     1621 gctgaaaatg cccggtcacc agctcgacga agcggtcgtg atccagattt cctgcggccg
     1681 agacgatcat gttggcgggc gcaaagcggt ggtggtaagc atcgatcacc gcctcgcgct
     1741 cgaagcgctt gacggtttct tttgttccca ggatcggctt gcccagagga tgatctttcc
     1801 agaagttctg cgtgaagatt tcgtgaacga gatagtcagg attgtcctcg tccatcttga
     1861 tctcttccat gatcacgccg cgttcgcggg cgatgtcggc ggcgtcgaaa accggatgca
     1921 gaactagatc gctcagaatg tcgagcgcag tgggcacatg ctcatcgagc actttcacgt
     1981 tgaagcagat gcactctttg gcggtgaatg catccatgtt gccgccgatg gaatcaacct
     2041 ggcgcgcgat ctcttccgcg ctgcgatgct tggtcccctt gaagaccatg tgttcgatga
     2101 agtgggagat gccattccac tcgggggctt catcgcgcga gccggtctgc agccagattc
     2161 caatcgacgc cgagcgaatg tgctgcatct gctcggtgat gatagtcagc ccattgggca
     2221 gcttttgacg acgaatattg cggacttcct gggccatgaa tcgatcccgt tgtgaaagaa
     2281 tactgcttaa cctgatagca cggactgcgg aaggatcttc aactattctg acataggcat
     2341 tggccgcgtg cgagaatagt ttagttgttt agaatcaaac ggttagattt ttcccacggt
     2401 ggaaccgccg ggccgcgagc acagctgctg gcaaccccag gccgtcggcc aagccgagga
     2461 ttccgtcgca tggcagaccc tgcacaaatc gcactgctgg ggctggaccg ctcggaaatt
     2521 gcctcgctga tcgaaggcat gggagagcct tcctaccgcg cgcagcagat tctcgatgcg
     2581 gtgtaccgcc agcgcttgga ttccatcgag cagatttcga ctttgccgca atcgcttcgc
     2641 tcgcgcctcg ctgaacaggg aattgccgtt ggtttgccgc agatcgagcg gcgattcgtt
     2701 tcgcaagacg gcacggtgcg ttatctcatg ggattggtcg acgggcaaag cgtcgaaacc
     2761 gtctggatgc cggaaggaga cggaggcgaa gagggagatg gaacggaagc cggcaatggt
     2821 gaaggctggg agcgcaccac catttgcgtt tcgagccagg tcggctgcgc cgtcgactgc
     2881 cagttttgcc tgacggcact gctgggcgtg aagcggaatt tgaccgcggg cgagattgcc
     2941 gggcaggttt gcgtcgttct gaaggatcag aaggtctcgc cgccgcagca ccgcatcaac
     3001 ctcgtgttca tgggcatggg cgaaccattc ctgaattatg acaacttcat gaaagcggtg
     3061 cgcttactgg tcgaagagac caatgtggcg gagtcgcgaa tgacggtttc aacggccggc
     3121 atcgtgccgc ggctttatga cttcggtcag gaaccccttc ggcccaagct ggctatctcg
     3181 ctgaacgcgc ccaatgatgc gctgcggaca gagctaatgc cgctgaatcg caagtggagc
     3241 ctggagaacc tgatcatggc tgcccgcgat tttcctctcc gcaatcgcga gcgcatcacg
     3301 tttgaatatg tgctcctccg cggcattaac gatgctactg agaatgcgcg ggaagtagtg
     3361 gagttgctgc gcgggctgcg ggctaaagtg aatctgatcg cgctgaaccc agggccggga
     3421 atcagcttcg aaacaccagc cgaggagcgc gtagtcgcct tccagcagat cttgatcaag
     3481 gcggggattc cggcgttcgt gcggcggccg cgaggccgcg acatttacgc tgcctgcggg
     3541 cagctcaagc gtacggttga gattgcagca gcgccgatac tgtagctctg tgaatgcccg
     3601 aggcgctagc agccggcaga tcgacgcgga cattcatggc gccgtcgctg cgagactcgc
     3661 agcaaatgtg accgtgatgc tccttcaaga ttccttcgca ggcactcagc gcaaggccat
     3721 cggtggcgtc gggcgctgtc tgcgcatcgc ctctggcaag ggaagccggc ggatcggtag
     3781 cgatctgcag cacgcagacc ccgtcgcaat tctccgtgtt aaccgtcagg agcctgctgc
     3841 cacgttcgct catcaggtgc aggcaatttc ccaggagttg caggcagacc tgaagcagtt
     3901 gattagaatc gcccagaact ttcggcagtg cgggatcaaa ttccgctcgg acctcgatat
     3961 taagggcctg ccattggggt tgcgtcagtt tgaccgcagt gcgggctaaa gtgttgaggt
     4021 cggtaggcgt ttgcggggct ggcgcctgct gggcgaagct gatcaggccg gccacgagc
//
LOCUS       DASPUL010000366         8310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1567557, whole genome shotgun sequence.
ACCESSION   DASPUL010000366 DASPUL010000000
VERSION     DASPUL010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8310)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567557"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1585
                     /locus_tag="VEJ00_08865"
     CDS             14..1585
                     /locus_tag="VEJ00_08865"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HXX01310.1"
                     /translation="MTEILLSTFCLALLSAFVLTRYVRDTATAHGWVAVPLQERHVHT
                     NPLPRLGGVAIFLSFSIAMAIAVFWVRHSPKIHASFSLKALLTILAPASLVFLLGVYD
                     DLRGAGPYLKFSIQGIGAVILFAGGLRIVNIPVLFGAHQLPWFVGLPFTVLWVLAITN
                     AFNLIDGLDGLAAGSALFSTLVAFVVALLNGPSLVTVTTLALAGAILGFLRYNFNPAT
                     IFLGDSGSLFIGFLLSALALKGAQKAPTIVAVAIPVVSFGLPILETLLSIIRRLISGR
                     PVFTADREHIHHKLLQHGLTHRQVVILLYGVSAIFALLSLFLLWPTGSSLGLVLAVLG
                     IGIWIGVQHLGYLEFGELARVAHRTLDQPQIFVNNLAIRRATEELKVARDYDQVRRIL
                     TAAFSSNDFDSFDLKLEPQSGEPVSSDPDAASAARRNPSSFRWSRPGASKLAEGAPVW
                     SIALDLLSSANRHRGTLTVQRRYSQSNLQLDINLLTSTFPTALADALDRTLTHGAQVI
                     ALPGQDAALVTAQAG"
     gene            1647..2756
                     /gene="wecB"
                     /locus_tag="VEJ00_08870"
     CDS             1647..2756
                     /gene="wecB"
                     /locus_tag="VEJ00_08870"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HXX01311.1"
                     /translation="MHILHVVGARPNFMKAAPVMRALAAHSGVQQSLVHTGQHYDDAM
                     SGIFFQQLEIPQPDCNLGVGSGSHAQQAAAVMIAFEPVLIKRRPDLVLVYGDVNSTVA
                     AALVCAKLGVRVGHVEAGLRSRDRSMPEEINRLLTDQLSDLLFTPSADADENLIAEGI
                     DRAKIHLVGNVMIDTLVRLLPRAEEYLPKNQSLPYALVTLHRPSNVDDVPWLRELLAT
                     LADLSGNLHVIFPVHPRTRQRLDGLASSHAANERLQLTEPMPYLQFLALQRRAAIVIT
                     DSGGIQEETTFLGVPCLTVRENTERPITLTRGTNQLVGRSPERLRAAAEEVLGRNSVP
                     RSEREPDKNRVPLWDGHAAGRLAQSIVRGRHDGWS"
     gene            3126..3980
                     /locus_tag="VEJ00_08875"
     CDS             3126..3980
                     /locus_tag="VEJ00_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXX01312.1"
                     /translation="MFRKTLYGTTFKDVSFVPLQSATFCVQCELISTSSKPYCLACGS
                     HALLGLSRVLGGSLRNQQTAHLITDAELDSLVRDLLRTVPDPTVLEAVDHSRMPSLAQ
                     RSQLRVANAAHPASGFRAVTIGEDIEIHAGELDLEPAISAITERAQHLTGATGAAIAL
                     RAGDEIVCRARSGRTAPDLGVRLQTDAGISAEAVRSGEVMLCHDAERNPRVDLVSCRR
                     LGVRSILVSPLRHYRRTLGVFEVLSSSPGAFDERDVATMQLLSSMMVAAISRISSLKR
                     VDRSRLAG"
     gene            complement(4017..5216)
                     /locus_tag="VEJ00_08880"
     CDS             complement(4017..5216)
                     /locus_tag="VEJ00_08880"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 domain-containing protein"
                     /protein_id="HXX01313.1"
                     /translation="MNRLRYGYVAVLWICCALGTGCGGSGGSKSNLITTSGSNVAPLS
                     VNSGPTGNDVNEAFVSVTICVPNTSTCQTIPNVLVDTGSPGLRIISSQLTVSLPQQMG
                     SNSQPVAECLPFLSGFTWGPVQTADIKIASETASAVPVQVISTTAFPVPSACSNMGLQ
                     PIETVAALGANGLLGIGLAAQDCGSACTQSGAANPGLYYECPAAGCQVTTEAVAQQVA
                     NPVGMFASDNNGVIFELPAVSGPEASLSGSLVFGIGTQSNNGLGSATVYTVDDFGNFT
                     TNFKGVSYPGSFLDSGSNGLFFLDSNTTGIPDCPSGDGFYCPSSTQNLSATNQGVNNA
                     SGNVNFSIANADSLFSNGNDAVFGNIGGPGLNSFDWGLPFFYGRNVFTAIEGKSTPGG
                     TGPFWAY"
     gene            complement(5213..5677)
                     /locus_tag="VEJ00_08885"
     CDS             complement(5213..5677)
                     /locus_tag="VEJ00_08885"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HXX01314.1"
                     /translation="MNRLLQTPGATALLVLLLALPGRMMASLGGRADSVQADQIHLQA
                     SLRTVAAQGYTMHELHAPTGMMVREFVSPAGVVFGLAWEGPWFPNMRQLLGSHFEEFT
                     QALSQNGSHGRRPIEVTLPTLVVHIAGHPRHYAGQAYLPDMLPQGMKAEEIR"
     gene            5910..7559
                     /locus_tag="VEJ00_08890"
     CDS             5910..7559
                     /locus_tag="VEJ00_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXX01315.1"
                     /translation="MAGTTSSVLSSIPNRRRRVRHRVQTPAYATFTGESKSAMLDLHE
                     IVDISEDGVSIRCPSPLDVNRRLNLCLDLAECSEHIYTTGQVIWSNPSGRTGIRFSGL
                     PPISLLRLREWLFLNAMSGLANADEAVLAPPVQDAVPPRPGYTDTLAALTAVQREVEA
                     LGSDLAGVLRLIASRAQTLVRASGAAIALSDSAPDFMICRASSGPDAPPVGARLQVGS
                     GFSGECVKSGRLLRCNDTELDARVDRESCRALGIRSILAAPVRMGVRTVGILEVFAPQ
                     PNSFGESDSNILQRLADTVLAAVNRAARAENLPLPGAPAPFRPSPGSVLFASAPPTDT
                     EKHSAKDDRGDEENSSGGVSLPLSHLVLLICTAITISMALGYHSGHYFAPWIQSRLQQ
                     RGHGQLPTVLASSQAPKSGDPGIETATFEQLQKMAGAGDPAAENALGRRYFEGDPENG
                     IRANEREAFHWFSRAAEDGNLAAQSKLGFLYWSGRGTPKDVNQAYFWTVLARARGDQG
                     NKDLAAVLASGMSRGQTIVIEQQADLWLQRHEGTTKPVAGH"
     gene            7606..>8310
                     /locus_tag="VEJ00_08895"
     CDS             7606..>8310
                     /locus_tag="VEJ00_08895"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXX01316.1"
                     /translation="MPTLRDILDQRVREADASLYQTLENQRVRCFSCGHCCPIPQGQP
                     GVCKVRYNRGGTLYVPWGYTAGMQCDPIEKKPFFHVHPGALAYSFGMMGCDLHCAYCQ
                     NWVTSQALRDPEAVSPPLEVSPEHLVREAVEQGARVVVSTYNEPLITSEWAVAIFKEA
                     KAAGLLTGFVSNGNATPQVLDYLRPWLDLYKVDLKSFDDRHYHELGGRIGPILDSIRR
                     IHQMGLWLEIVTLLIPG"
BASE COUNT         1531 a         2642 c         2377 g         1760 t
ORIGIN      
        1 cgagggggaa tagttgacgg aaattcttct gtcgacgttt tgtctggcgc tgctgtccgc
       61 cttcgtgctg acccgctacg tgcgcgatac cgcaaccgcc cacggctggg tggccgtgcc
      121 tttgcaagag cgccacgtcc ataccaaccc gctgccgcga ctgggcggcg tggcgatttt
      181 cctctccttc tcaattgcaa tggctattgc cgtcttttgg gtccggcaca gtccgaagat
      241 acacgcgtcc ttttcgctca aggcgctgct gaccatcctc gcaccggcct cgctggtgtt
      301 cctgctgggt gtgtacgacg atctgcgcgg cgcggggccg tatctcaagt tctcgattca
      361 ggggattggg gcggtgattc tattcgccgg agggctgcgg attgtcaaca ttccggttct
      421 ctttggcgct catcaactgc cctggtttgt gggacttccc tttacagttt tgtgggtttt
      481 ggccattacc aatgccttca acctgatcga cggactcgac ggtctggcgg ccggatcggc
      541 cctgttttca accttggtag ccttcgtggt cgcgctgctg aacgggccct cgctggtcac
      601 ggtaacgact ctcgcacttg ccggagccat tctcgggttc ctgcgctaca acttcaaccc
      661 cgccaccatc tttctgggcg actcaggaag cctcttcatc ggattcctgt tgagcgcctt
      721 ggccctgaag ggagcgcaga aggccccgac catcgttgcc gttgccatcc cggtggtttc
      781 cttcggactg ccgattctcg aaaccctgct ttccatcatc cgccgcttga ttagcggccg
      841 gccggtgttt accgcagacc gcgagcacat ccatcacaag ttgctgcaac acggtttgac
      901 ccatcgccag gtggtgatcc tgttgtacgg agtctccgcg atcttcgcgc tcctcagcct
      961 gttcctgttg tggccaaccg gcagctcgct cggcctggtg ctggcggttc ttggcatcgg
     1021 catctggatc ggcgtccagc atctcggata ccttgaattc ggcgagttgg cgcgcgtcgc
     1081 gcaccgtacg ctcgatcaac ctcagatttt cgtaaacaat ctggccatcc gccgcgcgac
     1141 cgaggaactg aaagtcgcgc gcgattatga tcaggtccgc aggattctca ctgccgcctt
     1201 cagcagcaac gacttcgaca gcttcgacct gaagctggag ccgcagtcgg gagagcctgt
     1261 ctcctccgac cccgatgcag catccgcggc gcgccgcaac ccgtcgtcct tccgctggag
     1321 tcgtccaggt gcttcaaaac tggccgaagg cgctcccgtc tggagcattg ccctcgatct
     1381 gctgagctcc gccaaccgtc accgcgggac gctgaccgtg caacgccgtt actctcagtc
     1441 caatttgcag ctcgacatca atctgctgac gtcgactttc ccgactgctc tcgcagacgc
     1501 tctcgatcgc accctcaccc acggggcgca ggtcatcgcg ctgcctgggc aagatgccgc
     1561 cttagttaca gcgcaggcgg gatagcccgc cgctcttcta ctccgaaacc agatacaatt
     1621 tccattcgcc ccgcgagcca gcccttatgc acattcttca cgtcgtcggc gcccggccta
     1681 acttcatgaa ggcagcaccg gtgatgcgcg ctctggcggc ccattccggc gtccagcaat
     1741 cgctggttca taccggacag cactatgacg acgccatgtc cggaatattc tttcagcagc
     1801 tggaaatccc gcaaccggat tgcaatctgg gagtcggctc cggaagccac gctcagcaag
     1861 cagccgcggt catgatcgct ttcgaacctg ttctcataaa gcgtaggccc gatctcgtac
     1921 tcgtgtatgg cgacgtgaac tccactgttg ccgctgcctt ggtctgtgcc aagcttggcg
     1981 tgcgggtcgg gcatgtggaa gccgggttgc gctcgcgcga ccgcagcatg cccgaggaaa
     2041 ttaaccgtct cctgaccgat cagctctctg atctgctgtt cacaccctcc gccgacgccg
     2101 atgagaatct gatcgccgaa ggcattgatc gggcgaagat tcacctggtc ggaaacgtca
     2161 tgattgacac gctggttcgc ttgctcccgc gcgctgaaga atatttgccc aagaaccagt
     2221 ctttgcctta tgccctggtt acattgcacc gtccttcgaa cgttgacgat gttccctggc
     2281 tgcgcgagtt gctggccacg ctcgcggact tgagcggcaa cctccacgtc atttttcccg
     2341 ttcatccccg cacccgccag cgactcgacg gcttggcttc ctcccatgct gcgaacgagc
     2401 gtctccagtt gacggaacca atgccgtatc tccagttcct cgcgctgcag cggcgcgctg
     2461 cgatcgtaat caccgattcc ggaggaatcc aggaggaaac cacgttcctg ggcgttcctt
     2521 gccttaccgt gcgcgagaac acggagcgtc cgattacgct gacgcgcgga actaatcagc
     2581 tagtcgggcg aagcccagag cggctgcgcg cggccgcgga agaagttttg ggcaggaatt
     2641 cggttccgcg gagcgagcgc gaacccgaca agaatcgtgt tcccctctgg gatggacatg
     2701 cagccgggcg ccttgcgcaa tccatagtac gaggtcggca cgacggctgg tcttaaagga
     2761 gagaaacttt gtctacagct ttgcgctggc gtaggagttg atttcgcagt tgaggcaaac
     2821 ttcttcaaaa gctggcgccg tccattccat gatttccccc cagacgttcg agaaacttca
     2881 ttaagcttaa ccgacccgaa tcctgaaatc aaccttccag aaaaattcac cgcacctcca
     2941 agcccttatc ctgcaagcct tttacaagac actgaactaa agtgcgaaag tcccgcgcga
     3001 ctcaacgcgc caagggcgag agcttccgct tttttccaca aggggaacct ttgccgccac
     3061 tttctctgta ggcgttccgt atttaccttt ctctgcaccg tgataaaagc gaggacccag
     3121 cacgtatgtt tcgcaagacg ttgtacggca ctactttcaa ggacgtcagt tttgttcccc
     3181 ttcagtcggc tacattctgc gttcaatgtg aactgatcag cacgagctcg aagccctatt
     3241 gcctcgcctg tggcagtcat gccttgctgg ggctttcgcg cgtgctgggc ggctcactgc
     3301 gcaaccagca gacagcgcat ctcattaccg acgccgagct cgacagcctg gttcgcgatc
     3361 ttctgcgcac tgtaccagac ccgacggtcc tcgaagctgt cgaccactca cggatgccgt
     3421 ctcttgctca gcgcagccag ctccgggtcg ccaacgcggc tcatcccgca tcgggctttc
     3481 gtgcggtgac tatcggtgaa gacatcgaga ttcatgccgg agagcttgat ctggagcccg
     3541 ccatcagcgc catcaccgag cgcgcccagc acctgacagg agcgaccggc gcggccatcg
     3601 ccttgcgtgc cggagacgag atcgtttgcc gcgcccgctc cggccgcacc gcgcccgatc
     3661 ttggcgttcg cctgcagact gacgcgggca tttcggctga agcggtgcgt tccggcgaag
     3721 tcatgctctg ccacgacgcc gagcgaaacc cgcgggtcga tctggtcagt tgccgccgcc
     3781 tcggagtgcg ctccatcctc gtctcgcccc tgcgccacta ccgccgaacg ctgggagttt
     3841 tcgaggtcct gtcttcttct cccggcgcat ttgacgaacg cgacgttgcc acgatgcaac
     3901 tgctctccag catgatggtg gcggcaattt cgcgcatctc cagcctgaag cgcgtagatc
     3961 gctctcgtct ggcggggtga agaccttcgg taaactgcag tagctttcgg ggcggatcag
     4021 tacgcccaga acggcccagt tccacccgga gtcgatttcc cttcaatcgc cgtgaagaca
     4081 ttccgcccat agaagaacgg caagccccag tcgaaagagt tcaaccccgg ccctccgatg
     4141 ttgccaaaga ccgcgtcgtt tccgttggaa aacaagctgt cggcgttcgc tatgctgaag
     4201 ttgacgttcc cgctggcgtt gttaacgccc tggtttgtag cggagagatt ttgcgtgctg
     4261 ctcgggcaat agaacccatc tccgctcggg caatcgggga tgccggttgt gttgctgtcg
     4321 aggaaaaaca atccgttgga accgctgtca agaaaacttc ccggatagga gactcctttg
     4381 aagttcgtag tgaaattccc gaagtcgtcc actgtataca ccgtcgcgct gcccaggcca
     4441 ttgttggact gagtcccaat cccgaacacc aaagaaccgc tgagcgaggc ctcgggcccg
     4501 ctgaccgcag gcagttcaaa tatgacgccg ttgttgtcgc tggcaaacat gcccacagga
     4561 ttcgccacct gctgagccac ggcttccgtg gtgacctggc atcctgcggc cgggcattca
     4621 tagtagagtc ccggattggc cgccccgctt tgcgtgcaag ccgacccaca atcctgcgca
     4681 gccaggccga ttcccagcaa cccgttcgcg ccgagtgcgg ccacggtttc gatgggctgc
     4741 aaccccatat tcgagcaggc gcttggcacg ggaaacgcgg tggtgctgat cacctgcacg
     4801 ggaaccgcac tggcggtttc actagcaatc tttatgtcgg ctgtctgcac tggcccccag
     4861 gtgaatccgc tcagaaaggg aaggcattcc gcgactggct ggctattcga ccccatctgt
     4921 tgcggcagcg aaacggtcaa ctgcgacgag atgatcctca accccggcga acccgtatcc
     4981 accaatacgt tcggaatggt ctggcacgtc gaggtatttg gaacgcaaat cgtgacgctg
     5041 acaaacgctt cgttcacgtc gttcccggta gggccgctgt tgaccgaaag cggagccacg
     5101 ttcgacccgg aggtcgtgat caagttcgac ttcgatccgc cgcttccccc gcaaccggtt
     5161 cccagtgcac agcagatcca taagacagcg acgtacccgt agcgaagacg gttcatcgga
     5221 tttcctcggc tttcatccct tggggcaaca tgtccgggag ataggcttgt cccgcgtagt
     5281 gacgcggatg tcccgctata tgcaccacga gcgtaggaag cgttacctcg atgggcctgc
     5341 gcccatggct tccgttttgg gacagcgcct gagtaaattc ctcgaagtgg gagcccagca
     5401 gttggcgcat attcggaaac cacggtcctt cccaggcgag tccgaacacc acgcccgcgg
     5461 gcgatacaaa ttccctgacc atcatcccgg tgggtgcgtg aagttcgtgc atggtgtagc
     5521 cttgcgcggc gacggtccgc aggctggctt gcaggtgaat ctggtcggct tgcaccgaat
     5581 ctgccctgcc accaagcgag gccatcatcc tgccaggaag agccagcagc agcacgagga
     5641 gtgcggttgc gccaggagtt tgcagaaggc ggttcataaa gactcctatg tactggtact
     5701 agaccgtatt tcgtaggctt ctaggttaag acgcgggctc gcgcctgcag gttgctagcc
     5761 ggagggcctc aagtatttgc ccgatccccg tcggccagag cctgggggtt cacttcccgc
     5821 tagagaattt cgactctaac ccgacttctt cgcatacttt tcgactaacc cgcgataata
     5881 tgtagggcga cagaccgggc cccgtcttta tggcaggtac gacctcgtca gtgctgtcgt
     5941 ccatacccaa ccgccgtcgc cgtgttcggc accgggtgca aactcctgcc tacgccactt
     6001 tcaccggcga atccaagagc gcgatgcttg atctccacga gatcgtggat atcagcgaag
     6061 acggagtctc catccgctgc ccgtctccgc tcgacgtgaa ccgtcgactg aatctttgcc
     6121 tggacctggc cgagtgctcg gagcacatct acaccacggg gcaagtgatc tggtcgaacc
     6181 cttccggacg cacagggatt cgcttttccg gattacctcc gatttcgctg ctgcgcttgc
     6241 gcgagtggct gtttctgaat gcgatgtcag gactggccaa cgcggacgag gcggtgctgg
     6301 cgccccctgt gcaggatgca gttccaccac gtcccggcta caccgacacg ctggccgctc
     6361 tgaccgcagt gcaacgggaa gtcgaagcgt tgggcagcga tcttgccggc gtcctgcgtt
     6421 tgattgcatc gcgcgcgcaa accctggttc gcgcttcggg cgccgccatc gctctttcgg
     6481 actcggcccc ggacttcatg atttgccgcg ccagttccgg gcccgatgcc ccgccagtcg
     6541 gcgcccgctt gcaggtggga tccggattct ccggcgaatg cgtgaaatca ggtcggttgc
     6601 tgcgctgcaa cgataccgaa ctcgatgcgc gcgtggatcg cgagagttgc cgcgccctcg
     6661 gcatccgctc cattctggct gctccggtgc gcatgggagt caggacggtc ggcattctcg
     6721 aagttttcgc gccgcagccg aattccttcg gggagagcga cagcaacatc ctgcagcggc
     6781 tcgcggatac tgttctggcg gcagtgaacc gcgccgcccg cgccgagaat ctgccgcttc
     6841 cgggcgctcc tgctcctttc aggccttcgc cgggcagcgt gttgtttgcc tcggcgccgc
     6901 cgacggatac cgagaagcat tcggcaaaag acgatagggg tgacgaggaa aacagttccg
     6961 gtggcgtaag cctgccgctc tcgcatctgg ttcttctgat ctgcacggcg atcacgatct
     7021 cgatggcgct gggctatcac tccggccact atttcgcccc ctggattcag agcaggttgc
     7081 aacagcgcgg ccatggccag ttgccgactg tactggcttc ttcgcaggct ccgaaatctg
     7141 gcgaccccgg catcgagacg gcaactttcg agcagctgca gaagatggcg ggggcgggcg
     7201 atccggctgc cgaaaatgct ctgggccggc gttattttga gggcgatcct gaaaacggca
     7261 tccgcgccaa tgagcgggaa gcctttcact ggttcagccg ggcggccgag gacggaaacc
     7321 tggctgccca gtccaagctg ggatttctct actggagcgg ccgcggaact cccaaggatg
     7381 tgaatcaggc ctatttctgg accgtgctgg cgcgggcgcg cggcgaccag ggaaacaagg
     7441 atctggccgc ggttctcgcc tcaggcatgt cgcgcggcca gaccatcgtc atcgaacagc
     7501 aggccgatct ctggctgcag cggcacgagg gcaccacaaa gcccgtggcc gggcactgag
     7561 ccccaggcgg gtttaaaggc gctagaatag tgtcaggggt gcgcaatgcc cacgcttcgg
     7621 gacattctcg atcaaagagt ccgcgaagct gacgcatcct tgtatcagac cctcgaaaac
     7681 cagcgcgtcc gctgcttctc ctgcggtcat tgctgtccca ttcctcaagg ccagccggga
     7741 gtctgcaaag tccgctataa ccgcggcggg acgctctacg tcccctgggg atacaccgcg
     7801 ggaatgcagt gcgatcccat cgagaagaaa cccttctttc acgtgcatcc cggcgccctc
     7861 gcatacagct tcggaatgat gggctgtgat ctgcactgcg cgtactgcca gaactgggtt
     7921 acgtcgcagg cattgcgcga tccggaagcc gtatcgccgc cgctcgaagt ttcgcccgaa
     7981 catctggtgc gggaagccgt tgagcagggt gcgcgggtcg tggtcagcac ctacaacgag
     8041 cctttgatca ccagcgaatg ggccgtcgct atcttcaaag aggcaaaagc cgcgggactt
     8101 cttaccggat tcgtttccaa cggcaatgcc acgccacagg ttctggatta cttgcgccca
     8161 tggctcgacc tttacaaagt tgacctcaag agtttcgacg atcgccacta ccacgaactc
     8221 ggcggtcgca tcggcccgat cctcgacagc atccggcgga ttcatcagat gggcttgtgg
     8281 ctagaaattg tcactctgct catccccggg
//
LOCUS       DASPUL010000367         3889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1019931, whole genome shotgun sequence.
ACCESSION   DASPUL010000367 DASPUL010000000
VERSION     DASPUL010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019931"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..660
                     /locus_tag="VEJ00_08900"
     CDS             256..660
                     /locus_tag="VEJ00_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01317.1"
                     /translation="MAYKETFWMACDSTEQLRAEYGPFHTRAEAESEAKKLGFGYLLR
                     YEHILDANEEIQDVRCIFIELPGAVPVQTEAVVVTLHTRCATCGESATHDQPWQAEVW
                     ADIHEFEHSRHRVRLFEHARGRGLKEIGDWRG"
     gene            complement(819..1571)
                     /gene="pgl"
                     /locus_tag="VEJ00_08905"
     CDS             complement(819..1571)
                     /gene="pgl"
                     /locus_tag="VEJ00_08905"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HXX01318.1"
                     /translation="MSASTEVRILTTPQELFAAAADEVLHSATEAVTQRGRYTIALSG
                     GSTPRNLYNLLATNARSSMPWDRTFFFWGDERHVSPADPESNYRMVEESMLSKAPVPA
                     GNVFRVAAENPDATAAADAYEKTLKSFFALEAGQLPRFDLILLGLGPDGHTASLFPGT
                     AALREESRLVVANWVEKFKTSRITFTLPVLNAARCVAFLVSGTDKAPALHAVLETDAP
                     GEQYPSKLVHPTDGKLIWLVDRAAASELSAKS"
     gene            complement(1589..2572)
                     /gene="glk"
                     /locus_tag="VEJ00_08910"
     CDS             complement(1589..2572)
                     /gene="glk"
                     /locus_tag="VEJ00_08910"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HXX01319.1"
                     /translation="MILAGDIGGTNARLAYFQSQNGHLRLISERVFPSREHHELGEIV
                     IQFLDESKAQPEAACFGIAGPVRNGRVETSNLPWVIEQSRLAQQIRLPATLLINDLEA
                     SAWGIGALGAGDLVSLNQPSGTATGNQAVIAPGTGLGQAGLFWDGTRHHVFACEGGHT
                     DFAPRDDLQIEMLRFLKTRFGHVSYERVLSGPGLVNVYEFLRESGCARESPQLAGELR
                     TGDPAATISRAALAGSCPLAVRALDLWISVYGAEASNLALKVMATGGLFLAGGISPKI
                     LPKLSGPLFMEAFLDKGRLRSLVESIPVQVVTNDKAGLLGAARCAAVRAKS"
     gene            complement(2569..>3889)
                     /gene="zwf"
                     /locus_tag="VEJ00_08915"
     CDS             complement(2569..>3889)
                     /gene="zwf"
                     /locus_tag="VEJ00_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXX01320.1"
                     /translation="HDDVKEFCSDCIESDLWEWFQRRFYYFAGDFTDDKVYPQLKDFL
                     VKVDQEHSAHQNFFYYLATAPSFFGPIVQKLSVAGLMEQNNSHWRRVIIEKPFGHDLE
                     SARALNQQLLRVATETQIYRIDHYLGKETVQNILAFRFANGIFEPVWNRRYIDHVQIS
                     VAETVGVEGRGSYFDQAGSLRDMVPNHIMQLISLTAMEPPISFDANAVRDEQAKILHA
                     IQPMTDEEVLSRTVRGQYGDGTEEGKRAVAYRAEPDVPPDSRTETFVATKLLIDNWRW
                     ADVPFYLRTGKHMPVRNTHIVIQFRRAPFVLFRDTPVEHLMPNQLVLHIQPEEGISLQ
                     FAAKVPGPVMRLGTVDMNFEYQEYFGKQPSTGYERLLHDCMIGDQTLFQRADMVEAGW
                     SVVNPILDIWKALPPRNFPNYASGTWGPKEADELLERDGRRWRNFEK"
BASE COUNT          771 a         1104 c         1250 g          764 t
ORIGIN      
        1 tcgagggcaa tacgcagtag agaggggcag ttggggagga aatgaagcgg gggtgtattg
       61 cagagccagc taaattctgc aaaaggtttc atgtccgcgg cctgcggaac ccggagccga
      121 tccgattaac tctatatctg gcaacggctt tcgacactcg ggtcctcggc ctcgaaattg
      181 cacgataagc cccgaatttt tctgcgcggg ctcagcagca tccacgagcc ggcttcgcag
      241 cgggggcgag gcaggatggc ctacaaagag actttctgga tggcgtgtga ctcgaccgag
      301 cagctgcgcg ctgagtatgg cccttttcat acccgcgccg aagccgaaag cgaagccaag
      361 aaactgggtt tcgggtacct gctgcgctac gagcacatcc tggacgccaa cgaagaaatc
      421 caggatgttc gctgtatctt cattgaactg cctggggccg ttccggttca gaccgaggcc
      481 gttgtcgtca ccctgcacac ccgctgcgcc acctgtggag agtcggcgac gcacgaccag
      541 ccgtggcagg ccgaagtctg ggccgacatc cacgagttcg aacactcgcg gcatcgggtg
      601 cgcctgttcg agcatgcccg gggccgaggt ttgaaggaaa tcggcgactg gcgaggataa
      661 gaggctttca ctgcagagga cgcagagctc gcggagactt caagagacct cggaagatgc
      721 gtgcatcgac gatgcacctg cctggtgcct gacttttcgc ccttttctgc tccaagactc
      781 tcgcggcgtg ttttgcgtgg tcggcgacag ttttgtcttc aactcttggc cgaaagctcg
      841 ctggccgcgg cgcgatctac cagccaaatc aacttgccat cggtggggtg caccagcttg
      901 gaaggatact gttcgcccgg agcatcggtt tcgagcacgg cgtgcagagc aggggccttg
      961 tccgttccgc tgaccagaaa agcgacacat cgcgctgcgt tcaaaaccgg gagagtgaag
     1021 gtgattcgac tggttttgaa cttttctacc cagttggcga ccaccaggcg cgattcttcc
     1081 cgcaaggcag ccgtgcctgg gaacaacgat gcggtgtgtc cgtccggtcc caagcccagc
     1141 agtatcaggt caaatcgcgg caactgtcct gcttcgaggg cgaagaagct tttgagcgtt
     1201 ttttcatagg catcggccgc ggccgtggcg tcgggattct ctgcggcaac gcggaacacg
     1261 ttgccggcgg gcaccggagc cttcgacagc atgctttctt cgaccatgcg gtagttgctt
     1321 tcgggatcgg cgggggagac gtgccgctcg tcgccccaga aaaagaacgt tcgatcccaa
     1381 ggcatggaag agcgggcatt ggtggcgagc aggttgtaca ggttccgggg ggtcgagccg
     1441 ccggagagag cgatggtgta gcgaccgcgc tgggttacgg cttcggtggc cgagtgcaaa
     1501 acttcgtcgg ccgctgccgc gaacaactcc tgcggcgtgg tcaggatgcg gacttcggtc
     1561 gaagcgctca cgtgaatgtt tgcagtgctc agctcttcgc gcgcaccgct gcgcagcggg
     1621 ctgccccgag caatcccgcc ttgtcgttgg tcacaacctg caccggaatc gattccacca
     1681 gcgagcgcag gcgacccttg tcgagaaaag cttccataaa cagcggacca ctcagcttgg
     1741 ggagaatctt gggcgagatg ccgccggcca gaaacagtcc ccccgtggcc atgactttga
     1801 gggccaggtt agaggcctcg gcgccgtaga ccgaaatcca caagtcgagc gcccttaccg
     1861 ccagtgggca gctgccggcc agcgcagcgc gcgaaatagt ggcagcagga tcgcccgtgc
     1921 gcaattctcc ggcgagctga ggagattcgc gggcgcatcc gctctcgcgg aggaattcat
     1981 atacattgac caagccagga cccgacaaga cgcgctcgta gctgacgtgt ccaaagcggg
     2041 ttttcaggaa tcgcagcatt tcgatttgca aatcgtcccg cggagcaaaa tcggtatggc
     2101 cgccttcgca ggcaaaaacg tgatggcggg ttccatccca gaacaacccc gcctggccga
     2161 ggccggttcc tggggcgatc accgcctgat tgcccgtcgc cgttccgctc ggctgattca
     2221 gcgagacgag atctcctgcg ccgagcgcgc cgatgcccca ggcgctggct tcgagatcgt
     2281 taatcaggag agtggcggga aggcgaatct gctgggcgag ccgggattgc tcgatcaccc
     2341 agggcagatt cgaggtctcg acgcgtccgt ttcgcaccgg gccggcgatg ccgaagcagg
     2401 cggcttcagg ctgcgccttc gactcgtcga gaaactgaat cacgatctcc ccgagttcgt
     2461 ggtgctcgcg gctggggaag acgcgctcgg aaatcaggcg cagatggccg ttctgggact
     2521 gaaagtaggc cagcctggca ttggtgccac ctatatcccc ggccagaatc atttttcgaa
     2581 attcctccag cggcggccgt cgcgttccag cagttcgtcg gcttctttcg gcccccatgt
     2641 gccggaggcg tagttgggga aattgcgggg cgggagggct ttccagatgt ccaggatcgg
     2701 gttgaccacc gaccagcccg cttcgaccat gtcagcgcgc tgaaacaggg tttggtcgcc
     2761 gatcatgcag tcgtggagaa ggcgctcgta acccgtgctc ggctgcttgc cgaaatattc
     2821 ctgatactcg aagttcatat cgaccgtgcc caggcgcatg acaggaccgg gaactttggc
     2881 ggcgaattgc agcgagatac cttcctcggg ctggatgtgc agcaccagct ggttgggcat
     2941 gaggtgctcg acgggcgtgt cgcggaaaag cacgaacgga gcgcggcgga actggatgac
     3001 gatgtgcgta ttgcgcacgg gcatgtgctt gccagtacgg agataaaaag gcacatcggc
     3061 ccagcgccag ttatcgatga gcagcttcgt ggcgacgaaa gtctcggtgc gagagtcggg
     3121 aggaacatcg ggctcggcgc gataggcaac ggcgcgcttg ccttcctcgg ttccatcgcc
     3181 gtactggccg cggacagtgc ggctgagcac ttcttcgtcg gtcataggct gaatggcgtg
     3241 caggattttg gcttgctcgt cgcgcaccgc attcgcgtcg aaggaaatcg gaggctccat
     3301 ggcggtgagc gagatgagct gcatgatgtg gttgggcacc atgtcgcgca gcgaaccggc
     3361 ctgatcgaaa tagctgccgc ggccttcgac gccgacggtt tccgccaccg aaatctgcac
     3421 gtgatcgata tagcggcggt tccagaccgg ctcgaagatt ccgttggcga aacgaaaggc
     3481 caaaatgttc tgcaccgtct ctttaccgag gtagtgatcg atgcggtaga tctgggtttc
     3541 ggtcgcgact ctcagcagtt gctggttcag ggcgcgggcc gattcgagat catgcccgaa
     3601 gggcttctcg atgattacgc gccgccaatg gctgttgttc tgctccatca ggccggcaac
     3661 ggagagtttc tgcacgatcg gaccgaagaa gctgggtgcc gtcgcgaggt agtagaagaa
     3721 gttctgatgg gccgagtgtt cctgatcgac cttgacgaga aaatccttga gctgaggata
     3781 gactttgtca tcggtgaaat cgccggcaaa gtagtagaag cggcgctgaa accattccca
     3841 gagatcgctt tcaatacagt cgctgcagaa ctccttgacg tcgtcatga
//
LOCUS       DASPUL010000368        11196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_65239, whole genome shotgun sequence.
ACCESSION   DASPUL010000368 DASPUL010000000
VERSION     DASPUL010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 11196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11196)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11196
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65239"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..625)
                     /gene="ybeY"
                     /locus_tag="VEJ00_08920"
     CDS             complement(110..625)
                     /gene="ybeY"
                     /locus_tag="VEJ00_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXX01321.1"
                     /translation="MVILQKKVAGLSQQALERFVLRARRAVRLRGGVNVMLTSSTAIR
                     VLNRQFRSQDKATDVLSFPLKSMRAGHGKTAALAGEIAISAEIAAQNAMRLGHSTAQE
                     VKILALHGVLHLAGFDHERDNGRMARKESALRRQLRLPAALIERAQRAKNESLHPGRG
                     SGRAPRPRRMA"
     gene            complement(692..1759)
                     /locus_tag="VEJ00_08925"
     CDS             complement(692..1759)
                     /locus_tag="VEJ00_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXX01322.1"
                     /translation="MKKSIGISPNIETLFGTRDENVRLLEDGLNVTITLRSNAIELEG
                     AARDVARAEQVFVDYESLQRSGYSFNNGDLNSMLRVLTSDPNSTLRGLADAGRQRSFG
                     RRTVQPKSNNQRRYLEAIEKHDMVFGIGPAGTGKTYLAVAMAISALLAKRVNRIILAR
                     PAVEAGERLGFLPGTLQDKIDPYLRPLYDALYDMLDPEKVDRYLEKNVIEIAPIAFMR
                     GRTLNDSFVILDEAQNTTSEQMKMFVTRLGFNSKAVITGDITQIDLPNARRSGLLEAI
                     EVLKNVNGLAFVHFDEGDVVRHHLVQRIVRAYDEHKARVAEEQMLLLTEAKAAEGAAA
                     NGKPASPAASEEKSPSAETQG"
     gene            complement(2015..3721)
                     /locus_tag="VEJ00_08930"
     CDS             complement(2015..3721)
                     /locus_tag="VEJ00_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXX01323.1"
                     /translation="MTDGLDRLKVLINSSTPIVVMETSEEMRAVSMVRAACAELNMAT
                     FEWSIADGLVRCGAGGSVETQKIPPQARVDQATAWTRVAHGQPPTRTVLSPGSGEAER
                     LTRAMMSAMGPESAAAAAAGGSIYNTREPVQALANMESMTIEAVFILKDFHRHMDDPV
                     VVRRLRDVGQKFSANRRTLVITAPELTVPPELATLVEYFDFPLPDRDRLHDIIHETFA
                     RLSKTYTLKLQLDAAGVDAMSANLRGLTEEEAERAISQAVVTRYALCPETITDVVEAK
                     KQLLRHSGMLEFIEAADNMASVGGLENLKRWLQQRRGAWEDSAREFGLEPPRGMIVLG
                     VQGCGKSLCARAVAGEWKLPLVKFDTSAVYDKYIGETEKRIRKVFQVAEGLAPCVLWI
                     DELEKVFAGSGPDSASADAGVSSRLLASFLSWMQDRKAPVFVAATCNNVTVLPPELIR
                     KGRFDELFFVDLPNQAERKQIFAIQLSKRKRDPADFDLEKLAAAARGYSGAEIDAAVQ
                     GGLYAAYSEKKQLTTQSLLDALAQTVPLSTTRAEEIAALREWARTRAVPASAPDSLRS
                     GT"
     gene            3832..4095
                     /gene="rpsT"
                     /locus_tag="VEJ00_08935"
     CDS             3832..4095
                     /gene="rpsT"
                     /locus_tag="VEJ00_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXX01324.1"
                     /translation="MANHLSALKRARQTERRTIRNRANTSALRTQLRDLRETITKGDK
                     TAAQQVYRKTVSALDKAIRKGLLHENTASRYKSRLGARVTAMK"
     gene            complement(4285..5409)
                     /gene="holA"
                     /locus_tag="VEJ00_08940"
     CDS             complement(4285..5409)
                     /gene="holA"
                     /locus_tag="VEJ00_08940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HXX01325.1"
                     /translation="MRAFAQAERFVSELESRKLRAAYVFVGDEAFFRKRFRDAILEHL
                     VPADLREFSLFEFDLAETDLAEILDRARTPSLMAPFQVFFVRGVKNLFGRGSNEEKLA
                     AIAEYCKNPNPAALLIFVADHISIPADVRRMEMQDKERYQRIRETMGQYCGIVELARV
                     EEGEAVRWIGDFCTSRDVKMEPDGARELVDSLGGDMMMISNELEKLILYAGEKRRITL
                     GDVETMVLAAKQRSLYELTDAISSRDRVRALEILDAILASGEGEEAAIGHVYMLAKTF
                     RQMLVILERNVRDQRMLWAALWQGFRVPPFAADDIIKQARRYKSRRELTRAIRLVAKA
                     DLALRSNPVSKRLVLEKLVMDLTTEPKVEAPGWMQHQLPV"
     gene            complement(5486..5980)
                     /locus_tag="VEJ00_08945"
     CDS             complement(5486..5980)
                     /locus_tag="VEJ00_08945"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HXX01326.1"
                     /translation="MLLLLLGTSCGYHTAGHSVQIPESVKTIAVPAFKNETTTYRIEQ
                     MLTTSVVREFTSRTHYRILNSPGEDADAILNGTVLSTVATPLAYDTASGRAASVLVVV
                     SIKVSLTDRHGKVLYQNPAYLFREQYELSQDLNSFFEEDSPAFRRLSQDFARTLVSNI
                     LEGF"
     gene            complement(6058..7134)
                     /locus_tag="VEJ00_08950"
     CDS             complement(6058..7134)
                     /locus_tag="VEJ00_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HXX01327.1"
                     /translation="MSETVKTTSGAHAVATRSNLYRAAIVGAASLKGKEIAEMLNERN
                     FPAVDVRLLDDDESLGQLEAMGDEISFIQSVRAEQFEHVDFTFFASDPDCTRKNWKRV
                     RDAGSAIIDVSGALENEPGASVRSPWVERERGQVVPPELQPGPCVTAHPVAVTLALLL
                     LRSAKAGAMRSIVATVFEPASEHGQRGMDELHQQTVNLLSFQPLPKDVFDVQVAFNMV
                     ARYGQKSQPPLDSAEARILRHYSKIAGEGAPHPSLMLLQAPIFHGHALAVFLEMERPV
                     DPQVLSQALAGDHVYIPGADEDAPSNVSSAGQPDIQLSLRADPVNPSGIWLWAAADNL
                     RISALTAVECAESMATTRPTGKIQ"
     gene            complement(7131..7997)
                     /locus_tag="VEJ00_08955"
     CDS             complement(7131..7997)
                     /locus_tag="VEJ00_08955"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HXX01328.1"
                     /translation="MDQAVPKPRRRRFSKGMYILPSLFTTANMGLGFYAILEVMHATL
                     ADPKLHLNNAAKAIGFAVLFDGLDGRIARMTGTTSDFGRELDSLADAITFGVAPAMLA
                     WTWGFFLMPPVLFTQGQWHIKLTQLGAIACFLFVMAGASRLARFNITSNPQPSNPGRP
                     GKKYFVGMPIPAGAGVIAAVVHFESGDPLVSWYTALTWIAMVTTVAYLMVSTWRFYSF
                     KDIDFRSRHPFRLIVLLAALFYGIAVFSGPVLFGIALLYMVSGVFWRLQWMFRRRTNP
                     PPPPYHEASHAS"
     gene            complement(7997..8662)
                     /locus_tag="VEJ00_08960"
     CDS             complement(7997..8662)
                     /locus_tag="VEJ00_08960"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HXX01329.1"
                     /translation="MVRDGYFYALGLIAAAVLVGWLASPAWAIIPMLLALFFLWFFRD
                     PERTIPQEAGAIVSPADGKVTDVSTVTAGNEKQTRLSIFLSVFDVHVNRSPIAGVVHD
                     IRYQRGKFLNAMNKASAEQNEQNIVTVEGDGQEVVFKQIAGLLARRIVFNPKIGDRVE
                     RGQRVGLIKFGSRVDVLLHASARVNVKVGDRVKGGASVLAYLEPQRELAVAGSGAASE
                     RTR"
     gene            complement(8823..9053)
                     /locus_tag="VEJ00_08965"
     CDS             complement(8823..9053)
                     /locus_tag="VEJ00_08965"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXX01330.1"
                     /translation="MLTPKDQLLTSHEEFRRLAQEHSQYSQRLDVLTQKRYLSEDEKL
                     EEVRLKKLKLRLKDQMESIEREYRQQYGVNVA"
     gene            complement(9153..9629)
                     /gene="rimI"
                     /locus_tag="VEJ00_08970"
     CDS             complement(9153..9629)
                     /gene="rimI"
                     /locus_tag="VEJ00_08970"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HXX01331.1"
                     /translation="MNVRHASVSDMPYLMSLERACATAAHWNDDRYRELLSFGERLVL
                     VAEEKGKARGKTIGEPGPAILGFLVARQVAAEWELENIVVAPASRGLGIGKRLLDALV
                     ARAREAGGEAVFLEVRESNAAALKLYEKAGFGLTGRRKSYYCDPVEDAILYRKALE"
     gene            9705..10088
                     /locus_tag="VEJ00_08975"
     CDS             9705..10088
                     /locus_tag="VEJ00_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXX01332.1"
                     /translation="MSNFIQITPFMHVPDMECALTFFTDTLGFKVLFRTANYAYIHRE
                     TVGFRLLEGKGPDAPPPGDRRFAYYIDVHDVDALHAQLKPRLDLLPKTDVHGPADKPY
                     GQRELLVLAPDGNLIAFGQEIRKPL"
     gene            complement(10107..10784)
                     /gene="tsaB"
                     /locus_tag="VEJ00_08980"
     CDS             complement(10107..10784)
                     /gene="tsaB"
                     /locus_tag="VEJ00_08980"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXX01333.1"
                     /translation="MILLVVDTSGKHGSVALARTSESAEATDVKVIESIPLAGGTFSA
                     QLVPQIAGLLNQHGLSKSDIDAFIVVSGPGSFTGLRVGLAAIKALAEILRKPIVPVSL
                     LEVIAIASRTQGRIAAALDAGRGEIYVGEYTVDGQSAQPLREQLLSKEEFLAARKGAV
                     ATPDAACAQAAREAGLPVFAVDPPNAEIIGSLGWSKLRNRDTVSPEQLEANYIRRSDA
                     ELFRKDT"
     gene            complement(10837..>11196)
                     /gene="mce"
                     /locus_tag="VEJ00_08985"
     CDS             complement(10837..>11196)
                     /gene="mce"
                     /locus_tag="VEJ00_08985"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HXX01334.1"
                     /translation="RSLSAAKAIYEKLGLNVSPEETVEHEQVRLVMVPVGESRLELLE
                     ATSENSTIAKFIAKRGEGLHHICLKVPDLPAAVVRLKKDGVRLVSEEIKAGAGGHQYV
                     FIHPAGTGGVLLELVQG"
BASE COUNT         2217 a         3477 c         3323 g         2179 t
ORIGIN      
        1 tggaagtcgc gcgacaggaa tttgccgatc tcctgataca cacgatccgc gtaggagacc
       61 agcgtcagca gtccgagcag gagaagcagc gcgagggcga ccgtcagact catgccatcc
      121 tccgcggtcg cggggcgcgc cccgagcccc gacctgggtg cagagattcg ttcttcgccc
      181 tctgagcacg ttcaatcagc gccgcaggca ggcgaagctg gcggcgcaac gcagattcct
      241 tccgcgccat tcgaccatta tcgcgctcat ggtcaaaccc cgccagatga aggaccccgt
      301 gcagggccaa gatcttgact tcctgtgccg tggagtgccc caggcgcatc gcattctgtg
      361 cggcaatttc tgcggagatg gcgatttcgc ctgccagcgc cgccgttttg ccatgcccgg
      421 ccctcatgga cttgagcgga aatgacagca catcggtggc cttgtcctgg ctgcgaaact
      481 ggcgattgag aacgcgaatt gccgtactgc tggtcagcat cacattcacc ccgccccgca
      541 gccgcactgc tcgccgcgcg cgcagaacga agcgctcgag tgcctgctgg ctcagcccag
      601 ctaccttctt ctgcaggatg accaaatgct ctctccgccg caaagcacac gggagggccc
      661 gctgcaggcc ctccctttga catctatcgc tctatccctg cgtttctgcc gacggcgatt
      721 tctcctcgct ggcagctggt gacgccggct ttccgttcgc cgcggccccc tccgccgctt
      781 tggcctcggt gagcaaaagc atctgctcct cggccacgcg cgccttgtgt tcatcgtagg
      841 cgcggacgat gcgctgcacc aggtgatgcc gcaccacatc cccctcgtcg aagtgtacaa
      901 acgccaggcc atttacgttc ttgagcacct cgattgcttc cagcagccca ctgcggcggg
      961 cgttgggcag gtcaatctgc gtgatgtcgc cggtgatcac cgccttcgag ttgaatccca
     1021 ggcgcgtgac gaacatcttc atttgctccg aggtcgtgtt ctgtgcctcg tcgagaatga
     1081 cgaacgaatc gttcagggtg cggccgcgca tgaaggcgat gggggcgatc tcgatgacgt
     1141 tcttctccag atagcgatcg accttttccg ggtcgagcat gtcgtagaga gcgtcgtaga
     1201 gggggcgcag gtaaggatcg atcttgtctt gcaatgttcc gggcagaaag cccagccgct
     1261 cgccagcctc gaccgccgga cgcgccagga tgatgcggtt cacgcgcttg gccagcagcg
     1321 cggaaatggc catcgcgacg gcgagatagg tcttgccggt gcccgccgga ccgatgccga
     1381 acaccatatc gtgcttttcg atcgcttcca ggtagcggcg ctgattgttg ctcttgggct
     1441 gaaccgtgcg ccgtccgaac gaacgctggc gtccggcatc ggccagacca cgcaaggtcg
     1501 aattcgggtc ggaggtcagc acgcgcagca tgctgttcag gtcgccgttg ttgaagctgt
     1561 aaccggagcg ctgcaggctc tcgtagtcca cgaagacttg ctcggcgcga gcgacatctc
     1621 gcgctgcccc ctccagttca atcgcgttcg agcgcagagt gatggtgacg ttgagcccgt
     1681 cttcaagcag gcgcacgttc tcatcgcggg taccaaacag ggtttcgata ttggggctga
     1741 tcccgatgct cttcttcata ttcgcaggct taaggccgca cggattgtgc tcttggaccg
     1801 atacgagcca aaccaggctg ggcagcctcg tgacgacaca gaattatgct cagagacggg
     1861 agtaagcgtg atgttcgcgc cacaagggcg agggaccagc attagctaaa gcctaccgcc
     1921 gtgacaacga gcccgtcaat ggtggctgct gcacaggcac ggcaccgagg tcactttggt
     1981 tcttccggcc gggctcgttg cgcgatgccg ataactaagt gcccgagcga aggctgtcgg
     2041 gagctgatgc aggcacagcc cgcgtcctcg cccattcccg cagagcggcg atctcctctg
     2101 ctcgtgtagt agagagtggc acggtctggg cgagggcatc cagcaaggat tgcgtggtca
     2161 actgcttttt ctcggagtac gccgcataaa gcccgccttg cacagcggcg tcgatttcgg
     2221 cgccggagta tcccctggcc gcagccgcaa gcttctccag atcaaaatcc gcaggatccc
     2281 gcttgcgctt cgacaactgg atggcgaaga tctgcttgcg ttccgcctga ttaggcaggt
     2341 cgacaaagaa cagttcatcg aaccgaccct tgcgaatgag ctcaggaggc agcaccgtca
     2401 cgttgttgca ggtggccgcg acgaacacgg gcgccttgcg atcctgcatc caggacagga
     2461 acgacgccag caggcgcgag gaaacgccgg cgtcggccga agccgaatcg ggtccgctgc
     2521 cggcgaagac tttttccagt tcgtcgatcc agagaacaca cggagccagc ccttctgcga
     2581 cttgaaagac tttgcgaatg cgcttctccg tctcgccaat gtatttgtcg tagacggcag
     2641 aggtatcgaa cttgaccagc ggcagcttcc actcgccggc gacggcccgc gcgcacagcg
     2701 atttgccgca tccctgaacg cccagaacga tcatgcctcg cggcggctcc agaccgaact
     2761 cgcgcgccga atcttcccag gctccgcgcc gctgctgtaa ccagcgcttc aggttctcca
     2821 gcccgcccac actcgccatg ttgtcggcgg cttcgatgaa ctcgagcatg ccagaatgcc
     2881 gcagcagctg cttcttggct tcgacgacgt cggtgatcgt ctcaggacaa agcgcatagc
     2941 gagttacaac ggcctgcgag atggcgcgct ccgcctcttc ttcggtcaac ccgcgcaggt
     3001 tggccgacat ggcatccaca cccgcggcat cgagctgcag cttcagcgta taggtcttcg
     3061 agagccgcgc gaaagtctcg tgaatgatgt catgcaggcg gtcgcgatcg ggcagaggaa
     3121 aatcgaaata ttcaaccagc gtggccagtt ccgggggcac ggtaagttcg ggcgcggtga
     3181 tcaccaaagt ccgccggttc gcagagaatt tctggcccac gtcgcgcagg cggcgcacga
     3241 ccacggggtc atccatatga cggtgaaagt ctttcagaat gaaaacggcc tcaatcgtca
     3301 tcgactccat gttggccagc gcctgcaccg gctcgcgggt gttgtagata gagccgccag
     3361 ccgccgcagc ggctgccgat tcggggccca tagccgacat catagctcgc gtcagccgct
     3421 cagcctctcc gctgccaggg ctgaggactg tccgtgtcgg tggctgcccg tgcgccacgc
     3481 gtgtccacgc cgttgcttgg tccacgcggg cctgcggcgg aatcttctgc gtttcgacgg
     3541 agccaccggc tccgcatcgg accaagccgt cggcgatgct ccactcaaac gtcgccatgt
     3601 tgagttcggc gcaggcagca cgcaccatgc tgaccgcacg catctcttct gaagtctcca
     3661 tcaccacgat gggcgtggag gagttgatga gcactttcag tcgatcgagg ccgtcagtca
     3721 tgggagcgtg tgagcgaggt gcgtttgacc gctggaaaat gactccctta taataagcac
     3781 ttcggacccg cccgaatatc tcgctcgaca cacgaaggta acgaaccaga catggctaat
     3841 catctatcgg cgctgaagcg cgcccgccag accgaacgac gcacgatccg caaccgcgcc
     3901 aacacctccg ccctgcgcac ccagctgcgc gacctgcgcg aaaccatcac gaagggcgac
     3961 aagaccgccg cccagcaggt ctaccgcaag accgtctccg ctctcgacaa ggcgatccgg
     4021 aagggcctgc tccacgaaaa taccgcctcg cgttacaagt cccgcctggg cgcacgcgtc
     4081 acggccatga agtagggtac ccgctgtcgg ctctgacccc acggctatca gcaatcagcc
     4141 cgaacagcat gcggttccgc ctttactcga tcttgtctct cttttgcaga ttgtgactcg
     4201 agcagagaat ttgaatgttc tttgcgtctt tcgaagagcc accctgcgag tagggatccg
     4261 ccgatggctg gaagcgtact cccctcacac cggcagctga tgctgcatcc agccgggagc
     4321 ctccacttta ggctcggtgg tcaggtccat gaccagcttt tccagcacga gccgtttgct
     4381 cacgggattt gagcgcaacg cgaggtcggc cttcgctacc agccggatgg cccgcgtcag
     4441 ctcgcgccgc gatttgtagc ggcgggcttg cttgatgatg tcgtccgcgg cgaacggagg
     4501 cacgcggaat ccctgccaca gcgcagccca cagcatgcgc tggtcgcgca cgttgcgctc
     4561 gagaatcacc agcatctgcc ggaaggtctt cgccagcatg tagacgtggc caatggccgc
     4621 ttcttcacct tcgcccgagg caagaatcgc atccaggatc tcgagcgcgc gtacgcggtc
     4681 gcgggacgag atcgcgtcgg tcagttcata gagcgagcgc tgcttggctg cgagcaccat
     4741 ggtttcgacg tcgcccagtg tgatgcgcct cttttctccc gcgtagagaa tcagcttctc
     4801 gagttcgttc gaaatcatca tcatgtcgcc gccgagcgag tccaccagct cgcgagcgcc
     4861 gtcgggctcc atcttcacgt cacggctggt gcagaagtcc ccaatccagc ggacggcttc
     4921 accctcttcc actcgcgcca gctcgacgat gccgcagtac tggcccatgg tctcgcggat
     4981 gcgctggtag cgctccttgt cctgcatctc catgcggcgg acgtcggccg gaatgctgat
     5041 gtggtcggca acgaagataa ggagcgcggc gggattcgga ttcttgcagt attccgcgat
     5101 ggcggccagc ttttcttcat tcgagccgcg gccgaaaaga ttcttcacac cccgcacgaa
     5161 gaagacctga aatggcgcca tcagcgaggg agttcgagcg cggtcaagga tctcggccag
     5221 atcggtctcg gcaaggtcaa attcgaaaag gctgaactcg cgcaggtcgg caggaaccag
     5281 gtgctccagg atggcatccc gaaagcgctt gcggaaaaaa gcttcatcgc ccacgaatac
     5341 gtaggctgcg cgcagcttgc gcgattccag ttcgctgaca aagcgctcgg cctgggcgaa
     5401 tgctctcacg acagtggtca gtgatcagtg gttagcgggc agtcaacaga ggaccgctgg
     5461 ctgctgatca ctagccactc taccatcaga acccctccag gatgttagag actagtgtgc
     5521 gggcaaagtc ctgcgacagg cggcggaaag ccggcgaatc ttcctcgaaa aagctgttca
     5581 aatcctgcga cagctcgtac tgctcgcgaa acaaatacgc ggggttctgg tagagaacct
     5641 tgccgtggcg gtcggtgagc gaaaccttga tgctcactac gaccagcaca ctggcggcgc
     5701 ggcccgaagc tgtatcgtag gccagcggcg tcgccacggt cgaaagcacg gttccgttga
     5761 ggattgcgtc ggcgtcctcg cccgggctgt tcagaatgcg gtagtgggtc cggctcgtaa
     5821 actcgcgcac caccgaggtc gtgagcatct gctcgatgcg gtaggtggtg gtttcgttct
     5881 tgaatgcagg cacggcgatg gtcttcacgc tctcgggaat ctgcaccgag tggcctgccg
     5941 tgtgatagcc gcagctggtg ccgaggagaa ggagaagcat cgagtaccaa gtagcgagta
     6001 ccgagcaaaa tctggtactc cggaccctcg ggactcgcga ctcgcgactc gcgactgtca
     6061 ttgaatcttc ccggtcggac gggtcgtggc catgctttcc gcacactcga cggcggtcag
     6121 cgccgagatg cgcaggttgt cagcagcggc ccacaaccag atcccgctgg ggttcactgg
     6181 atcggccctc agcgaaagtt ggatgtccgg ttgtcccgcc gagcttacgt tgctgggagc
     6241 atcttcgtcc gcacccggga tgtacacgtg gtcgccagcc agcgcctgcg acagaacctg
     6301 cggatcaacc gggcgttcca tctcaagaaa aaccgccagg gcatgcccgt ggaagatcgg
     6361 cgcctgcagg agcattagcg aaggatgggg agcgccctcc ccggcaattt tcgagtaatg
     6421 gcgaaggatg cgcgcttccg ccgaatccag cggcggctgc gacttctgcc cgtagcgcgc
     6481 caccatattg aaggcaacct gcacgtcgaa gacatctttc ggcaggggtt gaaacgagag
     6541 caggttcacg gtttgctggt ggagctcgtc catgccccgc tgtccgtgtt cggaagcagg
     6601 ctcaaagacc gtggctacga tgctccgcat tgccccggct tttgctgacc gcaacaagag
     6661 gagcgccagc gtaaccgcga ccggatgggc ggtaacgcac ggtccgggct gcaactcggg
     6721 gggcacaacc tgccctcgtt cgcgttcgac ccagggcgag cggacgctgg ccccaggttc
     6781 attctccagc gcaccagaaa catcgataat cgcgctgccg gcatcgcgca cccgcttcca
     6841 gttcttgcgt gtgcagtccg gatccgaggc gaagaaggta aagtccacgt gttcaaactg
     6901 ctcggcacga acgctctgta taaagctgat ctcgtcgccc atggcttcga gctggccgag
     6961 ggattcgtcg tcgtcgagca ggcgcacgtc aaccgcgggg aagttgcgct cgttcagcat
     7021 ctcggcgatc tctttgccct tgagcgaagc cgccccgacg atcgcagccc ggtacagatt
     7081 gctgcgagtg gcgacagcgt gcgcaccgga cgtggttttg accgtttcgc tcatgacgcg
     7141 tgcgaagcct cgtggtaggg aggaggtgga gggttcgttc gccggcgaaa catccattgc
     7201 agacgccaga aaacccctga gaccatgtag agcagagcta ttccgaacaa tacggggccg
     7261 gagaaaactg cgatgccata aaagagcgcc gccagcagga caatcagccg aaacggatgc
     7321 cgcgagcgga agtcgatgtc tttgaagcta tagaagcgcc aggtgctgac catcagatag
     7381 gccactgtgg tcaccatagc gatccaggtc agcgccgtat accaggagac aagcgggtct
     7441 ccgctttcga agtgcaccac cgcagcaatc acgcccgcgc ccgcaggaat tggcatgccg
     7501 acaaagtatt ttttgcccgg gcgtccggga ttcgaaggct ggggattgct cgtgatatta
     7561 aagcgcgcca ggcggctggc accggccatc acgaagagga aacaggcgat cgcgcccagc
     7621 tgcgtcagct tgatgtgcca ttgcccctgg gtaaacagca ccggcggcat caggaagaaa
     7681 ccccacgtcc aggcaagcat ggcgggagcg acgccaaagg tgatggcgtc ggccagggaa
     7741 tcgagttcgc ggccgaaatc gctggtcgtg cccgtcatac gggcaatgcg tccatcgagg
     7801 ccatcgaaca gtacagcgaa tccgattgcc ttggcggcat tgttgagatg cagctttggg
     7861 tcagcgaggg tggcatgcat gacctcgaga atggcgtaga agccgagccc catattggcg
     7921 gtcgtaaaca gcgaggggag aatgtacatc cccttgctga aacgccgccg ccgcggtttg
     7981 gggacagcct gatccatcaa cgcgttctct cgctggcggc cccactgccc gctaccgcca
     8041 gttctctctg aggctcaaga tacgccagca cgctcgcgcc ccccttgacc cggtcgccga
     8101 ccttcacgtt cacgcgggcg gaggcatgga gtaatacatc cacgcgtgag ccgaacttga
     8161 tcaggcccac gcgctggccg cgctccaccc ggtctccgat cttggggttg aacacgatgc
     8221 ggcgagccag tagtccggcg atctgcttga acacgacttc ctgtccgtcc ccctcgactg
     8281 tgacgatatt ctgctcgttc tgctcggcgg aagccttgtt catggcattg agaaacttgc
     8341 cgcgctggta acggatgtca tggaccactc ctgcgatcgg ggaacggttc acatgcacgt
     8401 cgaacacact gagaaagatg ctcagccggg tctgcttctc gttccccgcg gtcaccgttg
     8461 acacatcggt caccttgcca tcggcgggag aaacaatcgc gcctgcttct tgcgggattg
     8521 tgcgctccgg gtcgcgaaag aaccacagaa agaaaagcgc cagcagcatt ggaatgattg
     8581 cccaggctgg gctcgccaac catccgacga gaacagcggc tgcgatcagg ccaagcgcat
     8641 agaaatagcc atcacggacc atgggagtgg tttcattata gccgcgagcg gcctgcagac
     8701 ggggaatagc gccaaagttc cttgcggggc ttgtaagtct aaataagaaa aggcgcttcg
     8761 ctgcgccttg ggcaggatag attcctcccc tgcggccgag accgcgcgga ggctggtcct
     8821 gattaagcta cgttgacgcc gtactgctga cgatactcgc gctcaatgga ctccatctgg
     8881 tcctttaggc gcagcttcag cttcttcaga cggacctctt ccagcttttc gtcttcgctg
     8941 agatatcttt tttgggtgag tacgtcgaga cgttgcgaat actgcgagtg ctcctgagcc
     9001 agcctgcgaa attcttcgtg actggtcagg agttggtctt tcggagtcag catccagcag
     9061 acctccaaac cgggattatg gggccaaggt agcacagccc taaaactggt tcaatgccaa
     9121 aaaagtggtg cagtctcagg atgagaaccg ttttattcca gcgccttgcg gtacagaatt
     9181 gcgtcctcca ccggatcgca gtagtacgat tttcttcggc cagtcagccc aaagcctgct
     9241 ttttcataga gtttgagggc ggctgcgttt gactccctga cttccaggaa aaccgcttct
     9301 ccaccagctt ctcgcgcgcg agcaacgagc gcatcgagaa gccgcttgcc gattccgagc
     9361 ccgcgggatg caggggcgac aactatgttt tcgagttccc actccgcggc gacctggcgg
     9421 gcgaccagaa aaccaaggat cgctgggcct ggctcgccaa tcgtcttgcc ccttgctttc
     9481 cccttttctt ccgcgaccag aacaagcctt tcgccgaagc tgagcaattc tcgataccga
     9541 tcgtcgttcc agtgggccgc ggtagcacac gctcgctcca ggctcatcaa atagggcata
     9601 tcggaaacgc tggcatggcg aacgttcaag cggttaagat tcgttcctgt cattaggtcg
     9661 gatcgtccgg aggagtcagc gaacctcccg ggaaaggact cactttgtca aactttattc
     9721 aaattacgcc gttcatgcac gtccctgaca tggagtgcgc cttgactttc ttcaccgaca
     9781 cgctgggatt caaagtgctt tttcggacgg caaattacgc ctacattcac cgcgagacag
     9841 tgggctttcg cctgctcgag ggaaaggggc cggacgcccc gcctcccggt gaccggcgat
     9901 tcgcttacta cattgacgtg cacgacgtgg acgctcttca tgcccagctg aagcctcggc
     9961 tagatctgct acccaagacg gacgtccacg ggccagcgga caaaccctac ggtcagcggg
    10021 aactgcttgt gcttgcacca gacggcaacc tgatcgcctt cggccaggag attcgcaagc
    10081 cgttgtagat gcatccgcta tgagcatcag gtgtctttgc ggaacagttc tgcgtcggag
    10141 cgacgaatgt aattcgcctc caactgttcc ggcgagacgg tgtctcggtt ccgcaactta
    10201 ctccatccaa gactcccgat tatctccgcg ttggggggat cgactgcgaa aacgggaagg
    10261 ccagcctcgc gagccgcttg ggcgcaggca gcatccggcg tggccacagc ccctttccgc
    10321 gcggctagaa actcttcttt gctgagcaac tgctcacgca gaggctgcgc cgactggccg
    10381 tcaacggtat attcgccgac gtagatttcg ccgcgcccgg catcgagcgc ggccgcaatc
    10441 cgcccttgcg tcctgctggc gatggcgatt acctccaata gcgacaccgg cacgatcggc
    10501 ttgcggagaa tctccgccag agccttgatg gccgccaacc ccacgcgcag gccggtgaac
    10561 gaacccgggc cggaaactac gatgaatgca tcaatgtcac ttttgctgag accgtgttgg
    10621 ttgagcagac cggcaatctg cggcaccagc tgggcagaga atgttccacc ggcgagagga
    10681 atcgactcga tgaccttcac gtccgttgcc tctgcgctct cgctggtccg tgccaatgcg
    10741 acggagccat gtttgcccga agtgtcgacc accagaagaa tcatgaatga ctgtgaatcc
    10801 gcgggaatcc gcgaaaatcc gcggcaaaag aagatactaa ccctgtacca gttccagcaa
    10861 aacaccgcct gtgcccgcgg ggtgaataaa cacgtactga tgtccccctg cgccggcttt
    10921 gatttcttcc gaaaccagcc gcacgccgtc ttttttcagc cgcacgacgg cggcaggaag
    10981 gtcgggcact ttcagacaga tgtggtggag gccttctccg cgtttggcga tgaactttgc
    11041 gatggtcgag ttctcggagg tcgcctccag caactccagc cggctttcgc caaccggaac
    11101 catgaccagc cggacctgct cgtgttcgac cgtctcctcc ggcgagacgt tgaggccgag
    11161 cttctcataa atcgccttcg ccgctgacag cgacct
//
LOCUS       DASPUL010000369         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1020569, whole genome shotgun sequence.
ACCESSION   DASPUL010000369 DASPUL010000000
VERSION     DASPUL010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020569"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1068)
                     /gene="coxB"
                     /locus_tag="VEJ00_08990"
     CDS             complement(109..1068)
                     /gene="coxB"
                     /locus_tag="VEJ00_08990"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HXX01335.1"
                     /translation="MAGNVDALYIFLLIVAGMMTLLIFTAVLYFAARYRHRPGVPAEQ
                     IEGSIPLELTWSIIPLCVFMAIFLWSAVVYFRLRTPPRDSTEVYVVAKQWMWKLEHAE
                     GQREINELHVPVGRDVKLIMTSQDVIHSFFVPEFRMKQDVLPGRYTMAWFRATKPGTY
                     HLFCTQYCGTQHSGMIGSIIVMEPAQYEAWMSGGSTGPLSATGEKIFAELGCATCHRM
                     DTQGRGPNLQGVFGKPVLLEDGRLVTADENYVRESILDPGAKIVKGFKPVMPTFQGLV
                     SEEQLNALVAYVKSLSQSGQSGTAGTPAVAVPAEGSSKGSQVQ"
     gene            complement(1100..2041)
                     /locus_tag="VEJ00_08995"
     CDS             complement(1100..2041)
                     /locus_tag="VEJ00_08995"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXX01336.1"
                     /translation="MSSQNHNSTSTTSLVLSVVLLATTAWGQGMANGILSAPANVRPP
                     HLENVGIEQKLDAQVPRDLTFVDDAGRTVKLGDYFGKKPLILNLVYYNCTMLCGEALA
                     GLTSAMKLVKFDVGKEFEVVTVSFNPNETPTIAAAKKKDYLERYGRPGAAAGWHFLTG
                     PPESINALTKAVGFQYQYDPKINQYAHATAVMVLTPEGRISRYFYGVDFPPKDLRMGL
                     VEASQGKIGNLTDQVLLYCYHYDPETGKYGAIVANILKLGAAVTILLVGGMLLVLFRL
                     EKAAPPRRDWECSRPDQARPGQTGTTQTGPSRTHYVK"
     gene            complement(2038..2547)
                     /locus_tag="VEJ00_09000"
     CDS             complement(2038..2547)
                     /locus_tag="VEJ00_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01337.1"
                     /translation="MSDQIKPTNSTSHGGYERRDIGVAGVLYFLLGLAFAGLIVHFIV
                     TGLFSYLDKQSEAHQTPVSPLVTKAPADTRHLPPEYNGRYDEYLNKNFPAPQLEVDER
                     NQLDKIRLDEEQTLSTYGYVDQKAGTVRIPIDRAMDLIAQRGLPVRAQTPEGAAEATT
                     NNKKKGRKQ"
     gene            complement(2558..>3085)
                     /locus_tag="VEJ00_09005"
     CDS             complement(2558..>3085)
                     /locus_tag="VEJ00_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01338.1"
                     /translation="VIERILFNYKPMSEMLKPDFVHDHGKWMLTFIMVWAYFSFSQWL
                     IIWAGNLPTEITFYMKRLNGGWGSIGLFLVIFHFVVPFAILLSRPFKRDIRKLVWLAT
                     WLLLMRYVDLFWIIEPNFSKTITLTVADVVVPVAIGGIWLWFFFRNLSGLPLLPVYDP
                     TAVEVLEPAHHHGGA"
BASE COUNT          619 a          873 c          949 g          644 t
ORIGIN      
        1 agaggagcgc gatgcgcttg tggtccgtgg tcagcagcca cgagccgacg ccgtattcct
       61 tgttgagata attggtgcgc tcaggttgaa gattttctgt agccatactt attgcacctg
      121 actccccttg gatgaacctt ccgccggcac ggccacagcg ggcgtacccg ccgtcccgga
      181 ctggccggac tgcgacagcg acttcacgta tgcaaccagt gcattcagct gttcttcgct
      241 gaccagtccc tggaacgtgg gcatgacggg tttgaagccc ttcacgatct tcgcgccggg
      301 atcgagaatg gattcccgta cgtagttttc gtcggcggtg acgaggcgcc cgtcctcgag
      361 caacacgggc ttgccgaaaa ctccctgtaa atttggaccg cgaccctgcg tatccatgcg
      421 atggcaggtt gcgcagccaa gttcggcaaa gattttttcg cccgtggccg aaagtggccc
      481 ggtcgaaccc ccgctcatcc aggcttcgta ctgcgccggc tccatgacga tgatggagcc
      541 aatcatgccg gagtgctggg tgccgcaata ctgggtgcag aacagatgat aggttcccgg
      601 cttggtggcg cggaaccagg ccatggtgta gcggccgggg agcacatcct gcttcatgcg
      661 gaactcgggt acgaagaagc tgtggatcac atcctgcgaa gtcatgatca gcttgacgtc
      721 gcggcccacg ggcacgtgca gttcgttgat ctcgcgctgg ccctcggcat gttcgagctt
      781 ccacatccat tgcttggcga ccacataaac ttcggtcgag tcacgcggag gagtgcgcaa
      841 gcggaagtac acgacggcac tccaaaggaa gatcgccatg aacacgcaca gcggaatgat
      901 cgaccaggtc aactccagcg gaatagaacc ttcaatctgc tcagccggca ctccgggccg
      961 gtggcggtag cgcgcggcaa aatagagaac ggccgtaaag atcagcagcg tcatcatccc
     1021 ggccacgatc agcaggaaga tgtacagggc atccacgttg cctgccatgg tggaggcccg
     1081 ctgcggccat agtggaaagt tacttaacat agtgcgtgcg actcggtccc gtttgtgtcg
     1141 ttcctgtttg acccggtctc gcctggtctg gcctggagca ctcccaatct ctgcgcggcg
     1201 gcgcggcttt ctccagacgg aagagaacca gcagcattcc accgacaagc agaatcgtga
     1261 cagccgcgcc cagcttcaaa atgttggcca cgatcgcgcc atactttccg gtctcgggat
     1321 cgtagtggta gcagtagagc agcacctgat cggtaagatt gccaatcttg ccctgcgagg
     1381 cctcgaccag gcccatgcgc agatctttgg gcggaaaatc cacgccgtag aaatagcgcg
     1441 agatgcgtcc ctcgggtgtc aacaccataa cggcggtggc gtgcgcgtac tggttgattt
     1501 tcggatcgta ctggtactgg aagccgacgg ccttagtgag cgcattgatc gattcgggcg
     1561 gtccggtcag gaagtgccag ccggcagcgg cgcccggacg tccgtagcgc tcgaggtagt
     1621 ctttcttctt ggcggcagca atggtgggtg tctcgttggg attgaagctg accgtcacga
     1681 cttcgaattc tttacccacg tcgaacttca ccagtttcat ggcgctggtg aggccggcga
     1741 gggcttctcc gcagagcatg gtgcagttgt aatagacgag attcaggatt agtggcttct
     1801 tgccgaagta gtcgcccagt ttcacggtac ggccggcgtc gtcgacgaaa gtcagatcgc
     1861 gcgggacttg cgcgtcgagc ttctgctcga tgccgacgtt ttccagatgc ggcgggcgca
     1921 cgttggccgg agccgagagg atgccgttgg ccattccctg gccccaagct gttgtggcga
     1981 gcaacacgac cgacagcact aggctggtcg ttgatgtcga attgtgattc tgactgctca
     2041 ctgcttccgt cccttcttct tgttgttggt tgtcgcctcg gcggcgcctt ccggcgtctg
     2101 cgcgcgcacg gggaggccgc gttgcgcgat cagatccatg gcgcgatcga tcgggatacg
     2161 cacggttccg gctttctgat cgacgtaacc gtaggtgcta agcgtctgct cttcgtcaag
     2221 gcgaatcttg tccaactgat tgcgctcgtc gacctcgagc tgcggagcag ggaagttctt
     2281 gttgagatat tcgtcgtacc ggccgttgta ttcgggcggc aggtggcgcg tgtcggctgg
     2341 tgccttggtg accagcgggc tgaccggcgt ctggtgcgct tcgctctgct tgtccagata
     2401 cgaaaaaagt ccggtgacga tgaagtgaac gatgaggcca gcgaaggcca gcccgagaag
     2461 aaaatagagc actccggcga cgccgatgtc gcggcgctcg tatccgccgt ggctggttga
     2521 gttcgtgggt ttgatctgat cgctcatttg atctcgctca cgcacctcca tggtggtgag
     2581 ccggttccag cacttcgact gcagtcggat cgtagactgg cagcaaaggc aagccactca
     2641 ggttgcggaa gaaaaaccaa agccagattc cgccgatggc gacgggcacc accacatcgg
     2701 ccacagtcag cgttatggtt ttcgagaaat tcggctcgat gatccagaac aggtcgacgt
     2761 agcgcatcag cagaagccat gtggccagcc acaccagctt gcgaatgtcg cgcttgaacg
     2821 gacgcgagag cagaatcgcg aaggggacga cgaagtgaaa gatcacgagg aacagcccaa
     2881 ttgatcccca gccgccgttc aggcgcttca tataaaaggt gatttcggtc ggcaggttcc
     2941 cggcccagat aatgagccac tgcgagaagc tgaaataggc ccagaccatg atgaaggtca
     3001 gcatccactt gccgtggtcg tgcacgaagt cgggcttcag catctccgac atcggcttgt
     3061 agttgaacag aatgcgttcg atcac
//
LOCUS       DASPUL010000370         3802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1568433, whole genome shotgun sequence.
ACCESSION   DASPUL010000370 DASPUL010000000
VERSION     DASPUL010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568433"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1879
                     /locus_tag="VEJ00_09010"
     CDS             <1..1879
                     /locus_tag="VEJ00_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HXX01339.1"
                     /translation="VANPQEPVPTPSLLRASLQEKLPDYMVPATFVMLENLPLTPNGK
                     VDRKALPLPAAKDLERGVDPAVPRNETERILAHIWCQLLGLSHVGIHDNFFELGGDSI
                     VAIQIASRARHAGLKLTPRHCFLHRTIAELAAVTVSSRAVEADQGQVIGPVPMTPIQQ
                     WFFEQDCVEPQHWNQAILLAVAPTADAALFGPLIERLLEHHDALRMRFTRDGQSWRQI
                     NAATDAVSHFSVQDSSELEESEQRRTLESTAQQIQASLNLSKGPVFRAVFFDMGVNRP
                     GRLLLVAHHLVIDAVSWRILLEDFQVAYQQSRNRETVSLPAKTTSFRDWATRLCQYAQ
                     SEQPEKELAYWLGRPWSQVGHVRVDSSGGANDEASTRSSTVSLSDQETQTLLQKVPES
                     YRTGVDQVLLAAVAFVVAQWSSTPVVCIDVEGHGRQEDLIVGADVSRTVGWFTSVFPV
                     LLNTTWRNPAEALGVVREQFRTLPHRGIGYGILRYVCQEALARRLRDLPRPEISFNYL
                     GQFDRALSDSLLYAATESSGPSRSPRQKRPYLLEINGMVRDGKLQMRWTYSENVHRRE
                     TIDALADHCLAALRNLIIQRPSPGTGGDALPNFPLVRMIELTPEELETISATASKAGP
                     R"
     gene            1948..>3802
                     /locus_tag="VEJ00_09015"
     CDS             1948..>3802
                     /locus_tag="VEJ00_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HXX01340.1"
                     /translation="MKNIEGIYPLSATQEGMLFHTLYAPQSKMYFQQISFTVRGLDRS
                     AFRRAWEQVVNRHAALRSFFWLSDTKKAQVVCKGVHIDWDEQDWSELEFAGQQERLAN
                     YLQADRRRGIDVSRPPLMRCALMRLSPDTDQFIWSFHHMILDGWSCSVVLKEVFVLTE
                     SYCNGIDVQLEPLVPYQDYIAWLQRQDMHQAEEFWRRALKGFRAPTSLGIGHARKSVG
                     ATEFGEELCQLSAAATSALQEFSRQHQLTLHTLFQAAWALLLSRYSGETDVVFGTTVA
                     GRPPDLAGIESMVGLFINTVPFRVQVHSDQKVLPWLASLQAHMLEMQPYDYVPLVKVH
                     GWSEVPRKAPLFESILVFENYPLDRKTAAPGLRIGDVRSIERTNYPLTILVVPGEQLS
                     LRVSYRTETIDPQAVRRLLKHFDVVLEAFLTHKDQTLGHLPMLGEEERRQILVEWNDT
                     EVRFPDQDTCIHQLISRQAEAAADAVAVVFEDQQLTYGQLNRRANQLAHYLRKRGVGP
                     DVLVGICMHRSLELVVGLLGILKAGGAYVPLDPDYPPQRLAFMLADAQVGVLLTQERL
                     REALPPHGAELVCVDADWTAIEQEISTDPESTTTPDNLAYVMYTSGSTGTPK"
BASE COUNT          810 a         1100 c         1112 g          780 t
ORIGIN      
        1 cgtagcgaat ccgcaagaac cagtaccgac acccagcctg ttgcgtgcgt cgctccagga
       61 gaaactgccg gactacatgg tgcccgcgac cttcgtgatg cttgagaacc tgccgctcac
      121 cccaaatggc aaagtggacc ggaaggcgct acctcttccc gcggccaagg acttggagcg
      181 cggggtcgat ccggcagtgc cgcggaacga aacggaaagg atcctggcgc acatctggtg
      241 ccagcttctg ggcttgagcc atgtgggcat ccatgacaat ttcttcgaac tcggcggcga
      301 ttccattgtc gccatccaga tcgcttcgcg cgcgaggcac gctggcctga aactcacgcc
      361 tcgacactgt tttcttcacc gcacgattgc agagcttgcg gcagtgactg tcagttcacg
      421 agcggtcgaa gcggatcagg ggcaagtgat aggcccggtg cccatgacac cgattcaaca
      481 gtggtttttt gagcaggact gtgtcgagcc gcagcattgg aaccaggcga ttctattggc
      541 agtcgcccca acggccgacg cggcgttgtt cggcccgctg attgaacggc tgctggaaca
      601 ccatgacgcc ttgcggatgc gatttacgcg ggacgggcaa agctggagac aaataaacgc
      661 agcgaccgac gcggtgtcgc acttctccgt gcaagactcg agcgagctcg aggagtcaga
      721 acaacgcaga actcttgaat ccacggccca gcagattcag gcaagtctga acctgtcgaa
      781 aggtcccgtc tttcgtgcgg tgtttttcga catgggtgtg aatcgccccg gccgcctatt
      841 gctggttgca caccacttgg taatcgatgc cgtatcatgg cgcatcctgc tcgaggactt
      901 ccaggtcgcc taccaacagt cgcggaaccg ggaaacggta agtctccccg ccaagacaac
      961 ctcgtttcgc gactgggcga cacgcctatg ccaatacgcg caatcggaac aacccgaaaa
     1021 agagctcgca tattggctgg gccggccttg gagtcaagtc ggtcacgtac gtgtcgacag
     1081 ttctggcggg gcgaacgacg aggcctctac acgatcatcc acggtctcgc tcagcgacca
     1141 ggaaacgcaa accctcctgc agaaggttcc cgagtcgtat cggaccggtg tcgatcaagt
     1201 cctgctagca gcggttgcat tcgtcgtggc ccagtggagc agtactccgg tcgtatgcat
     1261 cgatgtcgaa ggacatggac gccaagaaga tttgatcgtc ggcgccgacg tatcgaggac
     1321 cgtgggctgg tttaccagcg tgtttccggt cctgttgaat acaacctggc gcaatccggc
     1381 cgaggcactg ggggtggtcc gggagcagtt tcgcacccta ccgcatcggg gaattggcta
     1441 cgggattctg cgctacgtgt gccaggaagc gctcgcacgt cggttgcgtg acctgcctcg
     1501 accagagatt tcattcaact acttgggaca gtttgaccgc gcgttatcgg attcgcttct
     1561 ctacgcagcg accgagtcaa gcggaccgag ccgaagcccc aggcaaaagc ggccgtactt
     1621 gctggaaatc aatggcatgg tgcgcgacgg aaaactgcaa atgcgctgga cctatagcga
     1681 aaacgtccat cgccgcgaga cgatcgacgc acttgccgac cactgcctgg cggcattgcg
     1741 aaatctgatc atacagcgtc cgtcgccggg caccggtggc gacgcgctgc cgaattttcc
     1801 gttggttcga atgatcgagc tcacaccaga agaattggaa acgatttccg ccaccgcctc
     1861 gaaagcaggt ccccgttagt tcgcgcaagg atccgagcac acagaaactc cattgagcac
     1921 tgaccacagt tcccgtgagg ccacatcatg aagaacattg aagggattta tcctctgtcg
     1981 gcgacgcaag agggcatgct atttcatacc ctctacgctc cgcagtcgaa aatgtatttt
     2041 caacagatct ccttcacagt gcgcgggctg gatcgctccg catttcggcg tgcctgggag
     2101 caggtggtga atcgacatgc agccttgcgt agcttctttt ggctgtcgga caccaaaaag
     2161 gcccaggtcg tgtgcaaagg tgtacatatc gattgggacg aacaggactg gagcgagcta
     2221 gagttcgctg ggcagcaaga gcgtctggca aattatctgc aagcggaccg gcggcgaggg
     2281 atcgatgttt ctcggccgcc cctgatgcgc tgtgcattga tgcgtctatc ccccgacacc
     2341 gatcagttta tctggagctt tcatcacatg attctcgatg gatggtcctg ctcggtggtg
     2401 ctgaaggaag tttttgttct caccgagagc tactgcaacg gaatcgacgt gcagctcgag
     2461 cccctcgtgc cgtatcagga ttacattgcc tggttgcaaa ggcaggacat gcaccaggct
     2521 gaggagttct ggcgtcgcgc actcaagggc tttcgggcac ctacgtcgct cgggatcgga
     2581 cacgcgcgaa agtcggtggg cgcaactgaa ttcggggagg agctctgtca gctatcggct
     2641 gcagcgacct ctgccttgca agaattttcc cgccaacatc aactcacctt gcataccttg
     2701 tttcaagccg cctgggcctt gctattgagc cggtatagcg gagagacaga tgttgtgttt
     2761 ggaacgacgg tcgcaggtcg gcctccggat ttggcgggga tcgagtcgat ggtggggctg
     2821 ttcattaata cagtgccctt tcgcgttcag gtccactcag accaaaaagt cttgccctgg
     2881 cttgcgtctc tccaagcaca catgctcgaa atgcagccct atgactatgt gcccttagtg
     2941 aaggtgcacg gatggagcga agtgccgcgc aaagcgcctt tgtttgaaag tatcttagtg
     3001 tttgagaact accctctcga ccgcaaaaca gcagccccgg gacttcgaat cggcgatgtg
     3061 cgttcgatcg agagaaccaa ttacccgctg acgatcttgg tcgtgccagg cgaacagttg
     3121 tcccttcgcg tgtcttaccg caccgaaacg atcgatccgc aagcggtccg acgcctgctc
     3181 aagcactttg acgtcgtgct cgaagcattt ctgacccaca aggatcaaac actagggcac
     3241 ttgccgatgc tcggcgagga ggaacgccgc cagatcttgg tcgaatggaa cgatacagaa
     3301 gtaaggtttc cagaccaaga cacctgcatt caccaactca tttcacgaca ggcggaggcg
     3361 gcggccgatg ccgttgccgt ggtgtttgaa gatcagcagc tcacttacgg ccaactcaac
     3421 cgcagggcca atcaactggc ccattatctc cgcaagcgtg gtgtcggacc cgacgtcctg
     3481 gtgggtatct gcatgcaccg ctcgctggaa ttggtcgtgg gcctgctcgg aatcttgaag
     3541 gccggcggcg cctacgttcc cctcgatccc gactatcccc ctcagcggct ggccttcatg
     3601 ctggctgatg cccaagtggg agtactgctg acccaggagc ggctgcgaga ggccttgcct
     3661 ccgcacgggg ccgagttggt gtgcgtcgat gcggattgga cggcgattga gcaggagatc
     3721 tcgaccgatc cggagagcac gaccacgccg gacaacctcg cctatgtgat gtacacctcc
     3781 ggatcgaccg gaacaccaaa gg
//
LOCUS       DASPUL010000371         5819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1568491, whole genome shotgun sequence.
ACCESSION   DASPUL010000371 DASPUL010000000
VERSION     DASPUL010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568491"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VEJ00_09020"
     CDS             complement(<1..207)
                     /locus_tag="VEJ00_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01341.1"
                     /translation="MLRDFKLIALTPPALPDPSLVRAAARAGASGTLDFEYVTDAQTV
                     GDALSKLAHLAARSCGVKFAGHKAE"
     gene            complement(286..1044)
                     /locus_tag="VEJ00_09025"
     CDS             complement(286..1044)
                     /locus_tag="VEJ00_09025"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HXX01342.1"
                     /translation="MNSTIQILDDEVHVWTASLVSSSQSLQGFWGELSGDERAKASRY
                     RFARDRDRYVAARGILRRLLREYTGIEPRTLQFEYSVYGKPSLKRGSVEKDFCFNLSH
                     SRDITVYAITGGAAVGVDVEYIHPEMAWTEIAGNFFSREEVQALRRLPLESRTLGFFS
                     CWTRKEAYIKARGEGLSLPLHHFQVSVDPYGPAELLSHFDDPSETNHWSLRQIVPVQG
                     YMAAVAAQMRSFTILNRMWPSASLFSSPRLAVHV"
     gene            complement(1123..5229)
                     /locus_tag="VEJ00_09030"
     CDS             complement(1123..5229)
                     /locus_tag="VEJ00_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HXX01343.1"
                     /translation="MNDRNLKLEAAAIHQPVDTSSIAQPGSSRLDIGSALRGGPLPVI
                     PRYANPSGALLSFAQERLWFLDLLQPNSSAYILACCVRLPGALDVEAFQRALSEVLVR
                     HESLRTTFSLADGSPVQAVGQPPPIHTKMFELIGLDSALQRAEILRICKEMQQPFDLE
                     RGPLVRANILRLQPEEYAALLTMHHMVSDGWSLRIFAQDLIALYQAFVSGKPSPLTDL
                     SIQYSDFARWQRSWLTGGVLDRELEYWKKQLAGVSTLNLPTDHPRSRLQSLRGAGEIR
                     IFESALSNQIRAQAQAQRVTLFMMFLAAFQILLSRYSGQQDIAVGFPVAGRSAAQTEP
                     LIGLFANTLVLRSDLSGNPTFRELLAKVRAVTLEALAHQDAPFEKLVELLRPDRDLSR
                     SPLFQVMFNFESHRAVKGTGPGTFIPLPMDSAAKFDLALTVVERERLSCYVQYNADLF
                     EAATIRRMLGHFEVLLVAAATCPDVRISDLPLLTPEERQQLVIERSQTRAEWNEPRCV
                     HELFESQAERKPRAIAVTYEKEHVTYGELNRQANQLAHHLCRRGVGVGTLVGVCLERS
                     IAMVSALLAIWKTGAAYVPLDPAYPAERLKFMLRDSDARFVMTQEPFGKIFAGCSAEI
                     IDCNQALPQVDKESSANPDAWVSAEDPAYVIYTSGSTGQPKGVEILHRSVTNLLESMR
                     RKPGMVESDTLLAITTLSFDIAVLELFLPLSTGAQLVIASREVAADPSALKRAIGTFA
                     PTVMQATPATWGMLVGAGWDGDENLRILCGGEAVSRELANTLVGRAREVWNMYGPTET
                     TIWSSTCQLHREDGPVPLGAPVANTEFYVLDGNRQLVPFGVPGELYIGGEGLARGYWK
                     RPELTDAKFVPHPLSDEPGRRLYRTGDLVRQWADGTLEYLSRIDNQVKIRGFRIELGE
                     IEAALMEQPGVRECVVIEREDRPGEKRLVAYLLGSKLSVPAVGELRTRLREKLPDYMI
                     PSATVILDAFPLTPSGKVNRLALPRPESNEPNREAGFLEPRDELELQLVGIWQRLLGH
                     RRIGVNDNFFELGGHSLLAVRLFVEIEKLLGRKLPLASLFLAPTIEQLAVLMREEGWS
                     PLWSSLVPLQVSGQASPFFCVHSLGSNLVSYRRLARFVGDDQPIYGLQPKGLDGKSDA
                     HTRVEDMAAHYLDEIRRVQPHGPYRLGGVCLGGVVAFEMAQQLVAQGEQVEKLVLIDS
                     HLPSHPEHFTTRAFHVGLFGRADLHLGEILLLPRKQKMSYILKRFQNLTSRGLRRLLD
                     TVGIANSRAREDAHFAEVLNKVRAANAQAEINYVPKFYPGRVTLFWCTGMATRSYQDR
                     RLGWSEVAGGGLEVQAIPGNHMTMVEEPHIKIMAAKLRVCLLGRLANS"
     gene            complement(5285..>5819)
                     /locus_tag="VEJ00_09035"
     CDS             complement(5285..>5819)
                     /locus_tag="VEJ00_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HXX01344.1"
                     /translation="VANPQEPVPTPSLLRASLQEKLPDYMVPATFVMLENLPLTPNGK
                     VDRRALPEPEPTRPELGNPYVAPQSPIEEILADIWAKVLRVEKVGVHDNFFELGGESL
                     LATQVIFRAREALEAELSLQTLFENPTIAQLAAAMTQAAAPSDTNLADEFRELGLLSE
                     NQHAKSIELRAQVNPTS"
BASE COUNT         1205 a         1717 c         1696 g         1201 t
ORIGIN      
        1 ctcggctttg tgtcctgcaa acttcacacc gcaggacctc gctgccaggt gggcgagctt
       61 gctgagtgca tcgccgaccg tctgcgcgtc ggtgacgtac tcgaaatcga gcgttcccga
      121 agctcccgcc cgcgccgcag ccctcacgag tgacggatcc ggcaatgcgg gtggtgtgag
      181 cgcaatcagt ttgaagtcgc gaagcatttg gccctctcga gattcagaac agtttctcgc
      241 ccgcatgaat ggcagggcag cgaagcgata gaatcgtgcg taccttcaga cgtgcaccgc
      301 aagccgggga cttgagaaaa gcgacgcgct tggccacatc cgattcagaa ttgtgaatga
      361 ccgcatttgg gccgcaacgg ctgccatata gccctggacc ggaacgatct gtcggagcga
      421 ccaatggttt gtttcagatg gatcgtcgaa atgcgagagc aactcggcgg ggccataggg
      481 atccaccgac acttgaaaat gatgcaaggg caggctcagg ccttctccac gcgccttgat
      541 gtaggcttct ttgcgagtcc agcaactaaa gaagccgagc gttctgctct ctagcggaag
      601 ccggcgcaac gcctgtactt cttcacgcga gaagaagttt cccgcgattt cggtccaggc
      661 catctcaggg tggatatact ccacatcgac gcccaccgcg gcaccgcctg taattgcgta
      721 gacggtgatg tcccgcgagt gcgaaagatt gaagcaaaaa tctttttcaa ccgatccgcg
      781 tttcaagcta ggcttgccat agacggagta ttcaaactgt agagtgcgag gttcgatacc
      841 ggtatactca cggagaagtc gtctgagaat cccacgggct gcgacatatc gatcgcgatc
      901 tctcgcaaag cgatagcggc tagccttcgc gcgctcatca ccggaaagtt cgccccagaa
      961 tccctggagg gactgtgacg aagagacgag agaagccgtc cagacgtgca cctcatcatc
     1021 gaggatttgg attgtcgagt tcacgaggtt ttgcgaccgc gtccggcggg catcggactc
     1081 gcgatgcggc gccgtgtaca gtcgcgacgc ggcttggctg agtcaggagt tcgcgagtct
     1141 accaagcaga cagactctga gttttgccgc catgatcttg atgtggggct cctctaccat
     1201 ggtcatgtgg ttgccgggaa tggcttgcac ctcaagtccc cctcccgcga cctcggacca
     1261 gcccagccgg cgatcttgat aggagcgcgt ggccattccc gtacaccaga atagggttac
     1321 ccgacctggg taaaacttgg gaacgtagtt gatttcggcc tgtgcgttgg ctgctcggac
     1381 tttgttcaag acctcagcga aatgggcgtc ctctcgggcc cgagaattcg cgatcccgac
     1441 cgtgtcgagc aagcgccgca gtcccctgct tgtcaagttt tggaaacgct tgaggatgta
     1501 cgacatcttc tgtttgcgag gcagcagcaa gatctcgcct aagtggagat cggctcgccc
     1561 gaacaggccc acatggaaag cgcgggtcgt gaagtgctca ggatgagaag gcagatggct
     1621 atcaatcagg accagcttct ccacctgctc tccctgcgcc acaagctgtt gcgccatctc
     1681 aaaggccact acgcccccca gacaaactcc cccgagacgg tagggcccgt gcggctgcac
     1741 tcgccggatc tcatcgagat aatgcgccgc catgtcttcg acgcgggtgt gggcgtccga
     1801 tttcccgtcc aaacctttcg gttgcaagcc ataaatcggc tgatcgtcgc cgacgaaacg
     1861 cgctaggcga cgatagctga ctaggtttga acccaacgaa tggacgcaga agaaaggcga
     1921 cgcttgtccg gacacctgca gcggaaccag ggaagaccaa aggggtgacc agccctcctc
     1981 acgcatcagc acggcgagct gctcaatggt cggggcaagg aacagcgagg ctaagggtaa
     2041 cttccgcccc aacagcttct cgatttcgac gaagagccgt actgcgagca gggaatggcc
     2101 gccgagctcg aaaaaattgt cgttcactcc gatgcgccgg tgaccgagca gccgctgcca
     2161 aatcccgacg agctggagtt cgagctcatc tcgaggctcg aggaaacccg cctcgcgatt
     2221 tggctcgttc gactctggcc gcggcaacgc tagccgattt acctttccgc tgggtgtcaa
     2281 gggaaaggca tcgagaatca ccgtcgcgct gggaatcatg taatcaggca gtttttctct
     2341 cagccgcgta cgaagctctc ccacagcggg tacactcagc ttgctgccga gcaaataggc
     2401 gaccagacgt ttctccccgg gccgatcttc tcgttcgatc acaacgcatt cccgcacgcc
     2461 gggctgctcc attagtgccg cttcgatctc ccccagttcg atccggaatc ctcgaatttt
     2521 tacttggttg tcaatccgag aaagatactc caacgttccg tcggcccact ggcgcaccag
     2581 atctcccgtt cggtacaagc ggcgtcccgg ttcatcgctc aaggggtgag gcacaaactt
     2641 tgcgtcggtg agttccggcc gtttccagta tccccgcgcg agcccctcac cgccgatgta
     2701 cagctccccc ggcacaccaa agggcactaa ctgtcgattg ccgtcgagta catagaactc
     2761 cgtattggca accggtgcgc ccagcggtac gggtccatct tctcgatgta gttggcaggt
     2821 ggaggaccag atagtggttt ccgtagggcc atacatgttc cagacctcac gggctcgccc
     2881 aaccaacgta tttgccaatt cgcgcgaaac ggcttcgccg ccgcaaagaa tccttaagtt
     2941 ctcgtcgccg tcccacccag cgcccacgag catgccccag gtcgccggcg tcgcttgcat
     3001 cacggtcggt gcaaacgtcc cgatggctct ttttaatgcg gaggggtctg cagcgacctc
     3061 acgactcgcg atcaccagct gcgcacccgt gctcaagggc agaaacagct ccaacacggc
     3121 aatgtcaaac gacagggtgg taatggccag cagcgtgtcg gattccacca tgcccggttt
     3181 ccgccgcatt gattcgagaa ggttggtgac ggaacgatgg aggatctcta caccctttgg
     3241 ctgtcctgtt gatccggacg tgtagatgac gtaggcggga tcttcggccg aaacccacgc
     3301 gtccggattg gccgaacttt ctttgtccac ctgcggcagg gcttgattgc agtcgatgat
     3361 ttctgctgag catccggcga aaatcttgcc gaacggttct tgcgtcatca caaatcgagc
     3421 gtctgaatcc cgcagcatga acttgagccg ctcggccgga taggccggat ccaggggcac
     3481 gtacgctgcg cccgttttcc atatcgccag gagcgcactt accatggcaa tcgaacgttc
     3541 taggcagaca cccaccaggg tgccgacacc cacacctcgg cggcagagat ggtgagcgag
     3601 ctggtttgct tgtcggttca gctcgccgta tgtgacgtgc tctttctcgt aggtcaccgc
     3661 gatcgcacgg ggtttgcgtt cggcttggga ctcgaacagt tcgtggacgc aacgcggctc
     3721 gttccattcg gcgcgggttt ggctgcgctc gattaccagc tgctgccgtt cctcgggtgt
     3781 caacagcggc aggtctgaaa tgcgcacgtc cggacaagtg gccgcagcga cgagcaaaac
     3841 ctcgaagtgg ccaagcattc tccggatcgt cgcggcctcg aacagatccg cgttgtactg
     3901 gacatagcaa gacagccttt ctcgttcgac cacagtcagc gccaaatcga acttcgccgc
     3961 cgagtccatc ggtaggggaa tgaaagtccc tggcccagtt cctttcaccg ctcggtggct
     4021 ttcgaaatta aacatcacct gaaatagcgg tgaacggctg aggtctctgt ccggtcgcaa
     4081 cagttcgacg agtttctcaa aaggagcatc ttggtgcgca agagcttcga gcgtgactgc
     4141 ccgcactttg gcaagcagtt cacgaaaggt cggattgccc gacaggtcgc tgcgcaacac
     4201 aagcgtgttg gcaaacagtc caatcaacgg ttcggtttgt gctgccgagc gcccggccac
     4261 cggaaaaccg acggcaatgt cctgctgtcc gctgtagcgg gagagcagga tttgaaatgc
     4321 cgccaggaac atcatgaaca gcgtcacgcg ctgggcttgt gcctgggccc taatttggtt
     4381 gctcaaggcg ctctcgaaga tccggatctc gccagcgccc cggagacttt gcagcctcga
     4441 gcgcggatga tcggtcggca agtttagtgt cgagacgcct gccagctgct ttttccagta
     4501 ttcgagctct cggtctagaa ccccgcccgt aagccaagat cgctgccatc gggcgaaatc
     4561 ggagtattgg atggaaagat cggttaacgg agagggtttg ccagatacga aggcctgata
     4621 gagagcaatc agatcctgcg cgaagattcg gagagaccag ccatcgctca ccatgtggtg
     4681 catggtgagc aaggccgcat actcctccgg ctgcagtcgc aagatattcg ccctcaccag
     4741 cggtcctcgt tccaggtcga agggttgttg catttccttg caaatgcgca ggatctcagc
     4801 cctttgcaga gcactgtcga gtccgatcag ttcgaacatc ttggtatgga tcggtggagg
     4861 ttgcccaacc gcttggaccg gggagccgtc tgccagagag aacgtggtcc gcagactttc
     4921 gtgacgaacg aggacctcgg agagggcgcg ctggaatgcc tcgacatcca gcgctcccgg
     4981 tagccgcaca cagcatgcca ggatatatgc cgaactattg ggctgcagta aatcaagaaa
     5041 ccagaggcgc tcctgcgcaa acgacaggag cgcgcctgac gggttcgcgt agcggggaat
     5101 gacaggcagt gggccaccgc gtagagcgct acctatgtcg agccgcgaag agcccggctg
     5161 tgctatcgag ctcgtgtcga ccggctgatg aattgcagca gcctcgagct tcagatttct
     5221 atcgttcatg tcgaggagag atcagccact tcggaggttg gcctgatcca acgatcgttg
     5281 aggctcaact cgtgggattc acttgcgccc ggagctcgat cgatttcgcg tgttggttct
     5341 ccgacagcag ccccaactcc cgaaactcat ctgccaagtt cgtgtcactc ggtgcagctg
     5401 cttgggtcat cgcggctgca agttgcgcaa tcgtaggatt ctcgaaaagg gtctgcagcg
     5461 atagctccgc ttcgagtgct tcccgtgcgc gaaagatcac ttgcgtggcg agcaatgact
     5521 cgccccccaa ttcgaaaaag ttgtcgtgta cgcccacttt ctctacccgc agtaccttag
     5581 cccagatgtc ggcaaggatc tcctcgatcg ggctttgtgg agcgacgtac gggttgccaa
     5641 gttccggtcg cgtcggctcc ggttcgggga gtgctcggcg gtccactttg ccatttgggg
     5701 tgagcggcag gttctcaagc atcacgaagg tcgcgggcac catgtagtcc ggcagtttct
     5761 cctggagcga cgcacgcaac aggctgggtg tcggtactgg ttcttgcgga ttcgctacg
//
LOCUS       DASPUL010000372         6083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_617962, whole genome shotgun sequence.
ACCESSION   DASPUL010000372 DASPUL010000000
VERSION     DASPUL010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6083)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6083
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617962"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VEJ00_09040"
     CDS             <1..330
                     /locus_tag="VEJ00_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXX01345.1"
                     /translation="LNLVGNAIKFTENGEVAIRVNVDDGAFLAAVADTGPGIKKEDQQ
                     KIFEEFQQSDTSVTKSKSGTGLGLAIAKRIVELHGGRIWVESVLGRGSTFFVFLPIVA
                     AASAARG"
     gene            357..1364
                     /locus_tag="VEJ00_09045"
     CDS             357..1364
                     /locus_tag="VEJ00_09045"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXX01346.1"
                     /translation="MTAHWLHRYRILLILAAATAAFRVPIAFAAEPTQRLVRVGFVDP
                     ASPSTRPRGVEGFWQHLRELGWVEGKNMVIEARSADGQDDRLPALMTEVLGTKVDVLV
                     TYGTPAAMAAKRATSTIPIVDAAMADPVASGLVTSLAHPGGNFTGLSLAWAGGMAGKR
                     LELLQETVPHLASVAMITGADDPQVFREMAKEVEKIAPTRGIKVRHIEVRDAGALDRT
                     FKQARKEAQALLIPGGLFMFEHRREATALAAKYKLPAIYPVRDYVEAGGLMAYGTDIG
                     ILFRRAADYVDKILRGAKPGDLPIEQPTKYELVVNLKTAKALGITIPESILLRADEVI
                     R"
     gene            1485..2597
                     /locus_tag="VEJ00_09050"
     CDS             1485..2597
                     /locus_tag="VEJ00_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HXX01347.1"
                     /translation="MQARAAVAWQAGKPLSLETVELEGPKAGEVLVEIKATGICHTDE
                     YTLSGSDPEGLFPTILGHEGAGVVVDVGPGVKSLKKGDHVIPLYVPECRECEYCVSQK
                     TNLCQSIRVTQGQGLMPDGTSRFSLGGKKLHHYMGTSTFSNYTVAPEIALAKIREDAP
                     FDKVCYIGCGVTTGIGAVINTAKVEPGANVVVFGLGGIGLNVIQGARMAGANMIVGVD
                     VNAGKKALAQKFGMTHFVNPKDMPEQELVPYLVNLTKGGADYSFECIGNVKVMRQALE
                     CCHKGWGVSVIIGVAGAGQEIATRPFQLVTGRVWKGTAFGGARGRTDVPRIVDWYMDR
                     KINIDDLITHTLKLEDINRGFDLMHEGKSIRAVVVY"
     gene            2838..3701
                     /locus_tag="VEJ00_09055"
     CDS             2838..3701
                     /locus_tag="VEJ00_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01348.1"
                     /translation="MQKLLVYQSLWAMERRRPDGLEWSLPEKLQMIRDAGFDGAGVRF
                     FDYKYAKEVTEFLRANNMTWQAQCYPKTIDDLKPIIEHVKELGADHINLQADVRPYRV
                     EDCIPYIEGWRRLARDAGIAMHMETHRDRMTTDLFFTLHLLDCFPDLRLTGDISHYVV
                     GREFAWPISEENHRLMHRILDNTWGFHGRVASREQVQIQISFPHHKDWVDLFLGWWEY
                     GFRSWRKRAPRDATLTFLCELGPKEYAMTGPDGYELSDRWEEAKMMKDMVRTLWRRLE
                     EDERAPATRAA"
     gene            3845..4432
                     /locus_tag="VEJ00_09060"
     CDS             3845..4432
                     /locus_tag="VEJ00_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01349.1"
                     /translation="MERIWNKRTFERRLNPNPVDVPIDLYAALISTPEVVAAAANRLG
                     LTSSTVTVVDDRTYEWRTAEGSRALYRILLSEPRHQVTLFQGHLIMYGLTVHGSMLGN
                     LDLSNDSTGVHQDLSVYVQVDRSIMAWLTKLFVALIPGKIDAEVSHGFEFTGAVARWA
                     WRDREGFCRWRQTSAFAPERTARIAELVGCATVVH"
     gene            complement(4524..5387)
                     /locus_tag="VEJ00_09065"
     CDS             complement(4524..5387)
                     /locus_tag="VEJ00_09065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXX01350.1"
                     /translation="MSDDNAVVVEAWNTVLYDKFCRFKHLLIDGLSAHSNEALSRGGY
                     PEGSRVLDVGCGFGDSTRLIAKRVGPKGEAVGVDCAENFIKSAQQESRETGVGNASFF
                     VADVQADDLRGPYDFAFSRFGTMFFMLPGAALRNIRKALKPGGALTMIVWRRREENPW
                     VHDAELCVKEIVPVVSHEDTDEVHCGPGPFSMAGPDMVSSMLRGSGYDRIAFERFDAD
                     ICIGKDMDEAIEFAMALGPAGEIIRLAGEEGAKRKDQVAAALRKTLSHYTRGNGVWAP
                     SSTWIVTARNP"
     gene            5457..>6083
                     /locus_tag="VEJ00_09070"
     CDS             5457..>6083
                     /locus_tag="VEJ00_09070"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HXX01351.1"
                     /translation="MSGERMQWRVKIGTDETAAVFEAASTGSRGRLFVCAHGAGGHMA
                     DRGMLALSEVFRGRGTDVVRFNFLYREKGSSKPDAMPRLEECVSAVVSRARQEVQPDL
                     LFIGGRSMGGRAASMLAADGFSCDGLILLAYPLHLAGQPEKLRSAHLVQIKVPVLSLN
                     GTRDSLCTRELMDQVTGGLSSVWTMHWLEGADHSFHVLKSSGRSDGDVL"
BASE COUNT         1181 a         1810 c         1986 g         1106 t
ORIGIN      
        1 ctcaaccttg tcggcaacgc catcaagttc acagaaaacg gcgaggtcgc gattcgggtg
       61 aacgtcgacg atggcgcgtt cctggcagcg gtcgccgata cgggacctgg catcaagaag
      121 gaagatcagc agaagatctt cgaagagttc cagcagagcg atacttctgt caccaagagt
      181 aagagcggca ctggactggg gctggcgata gcaaagcgca tcgtggagct gcacggcggg
      241 cgcatctggg tagaatccgt cctcggccgg ggctcgacgt tcttcgtatt cctgccgatc
      301 gtggctgctg ccagcgccgc acgcggataa tcatctagga agggattcag gcgttcatga
      361 ccgcgcattg gctgcacaga tatcggatcc tcctgatcct ggccgcggca acagccgcat
      421 tccgggttcc gattgcgttc gcagcggagc ccacgcaaag gctggtgcgc gtcgggttcg
      481 ttgatccggc gtcgccctcc acccgccctc gtggcgtcga agggttctgg cagcatctgc
      541 gcgagctggg ctgggtagag ggtaagaaca tggttatcga ggcacgctcg gccgacgggc
      601 aggacgaccg gcttccggcg ctcatgaccg aggtactcgg gaccaaagtc gatgtgctcg
      661 tcacctacgg cacgcccgcc gcaatggccg caaagcgcgc cacgagcaca attccgattg
      721 tcgacgcggc catggccgat cccgtagcga gcggtctcgt cacaagcctt gcgcaccccg
      781 gcggcaactt tacgggcctg tcactcgcgt gggctggggg aatggcgggc aagcggttgg
      841 aactgctcca ggagacagtt ccacacttgg ccagcgtcgc tatgattacg ggcgctgacg
      901 accctcaagt gttccgagaa atggcgaaag aagtcgaaaa gatcgcgcca acgcggggca
      961 tcaaagttcg gcacatcgag gtccgcgatg cgggggcgct cgatcgtacc ttcaagcagg
     1021 cgcgcaagga ggctcaggcg cttctgattc caggcggcct cttcatgttc gagcaccggc
     1081 gcgaagcgac agcgctcgcc gctaagtaca agctccctgc catctatccg gtgcgcgact
     1141 acgtcgaggc aggcgggctt atggcatacg gcacggacat agggatcttg tttcgccgtg
     1201 cggccgacta tgtcgacaag attctgaggg gggcgaagcc aggggacctg cccatcgagc
     1261 agcccactaa gtacgaactg gtggtcaacc tgaagactgc caaagcgctg ggcatcacca
     1321 tccccgaatc gatcctgctg cgcgcggacg aggtaatccg gtgagtctct gccacagtgc
     1381 gggcgaggga gcaacgccgt tgacttcccc ggatcgggtg gttaccgtac aagcagcgat
     1441 ccgcgctgag ccggttcgct tgtaatcgca accgggggga gtccatgcaa gcacgcgccg
     1501 cagtcgcctg gcaggcgggg aaaccgcttt cgctggaaac cgtagaactg gaggggccca
     1561 aagccggcga ggtcctggtc gagatcaaag ccacggggat ctgccacacc gacgagtaca
     1621 cgctgtcggg ttccgatccg gaggggttgt ttcccaccat actcgggcat gagggggcgg
     1681 gcgtcgtggt cgacgtcggt cccggcgtga agagcctcaa gaagggcgat cacgtcattc
     1741 cgctgtacgt tccggaatgc cgcgagtgcg agtactgcgt ctcgcagaag accaatctgt
     1801 gccagtcgat ccgcgtcacc cagggccagg ggttgatgcc tgatggcacc agccgctttt
     1861 cgctcggcgg caagaagctc catcactaca tgggcacatc gaccttctcc aactacacgg
     1921 tcgcgccgga gatcgcgctg gcgaagatcc gtgaggacgc gcccttcgac aaggtgtgct
     1981 acatcgggtg cggcgtgacg accggcatcg gcgcggtgat caacacggcc aaagtcgagc
     2041 cgggcgccaa tgtcgtggtg ttcggtctcg gcggcatcgg gctgaatgtg attcagggcg
     2101 cgcgcatggc gggcgcgaac atgatcgtcg gcgtggatgt caacgccggc aagaaggcgc
     2161 tcgcgcagaa gttcgggatg acgcacttcg tgaacccgaa ggacatgccc gagcaggagc
     2221 tggtgcccta cctcgtgaat ctcacgaagg gcggcgccga ctacagcttc gagtgcatcg
     2281 gcaacgtcaa agtgatgcgc caggcgctcg agtgctgcca caagggctgg ggcgtgtcgg
     2341 tcatcatcgg cgtggcgggc gccggccagg agatcgcgac ccgcccattc cagcttgtca
     2401 cgggccgtgt gtggaaaggc accgcgtttg gcggtgcgcg cggacgcacc gatgtgccgc
     2461 gcatcgtcga ctggtacatg gaccgcaaga tcaacatcga cgaccttatc acgcatacgc
     2521 tgaaactgga ggacatcaac cgcgggttcg atctcatgca cgagggcaag tcgatccggg
     2581 cggtggtcgt gtattaaggc cccctcaccc taaccctctc ccgcaagggg agaggggacg
     2641 gtaaagcggt ccgcgcaagg ggagagggga cggtaaagcg gtccccgcga ggggagaggg
     2701 gatggcaaag cggtcggcaa ggggaaaggg gaccgacgga aagggcactg ccgtgcagta
     2761 gcactccctc tccctcgacg ggagagggca gggtgagggt gagagaagga atcgcgctcg
     2821 acgaaggggg tatagccatg caaaagcttc tggtctatca atcgctctgg gcgatggagc
     2881 ggcgccggcc cgacgggctc gaatggtcgc tgccggaaaa gctgcagatg atccgcgacg
     2941 ccggctttga cggcgcgggg gtgcgcttct tcgactacaa gtacgccaag gaggtcaccg
     3001 agttcttgcg cgccaacaac atgacctggc aggcccagtg ctacccgaaa acgatcgacg
     3061 atctgaaacc gatcatcgag cacgtgaagg agctcggcgc cgaccacatc aacctgcagg
     3121 cggacgtgcg gccgtaccgc gtggaagatt gcattcccta catcgagggc tggcggcgcc
     3181 tcgctcggga tgccggcata gcgatgcata tggagacgca tcgagatcgg atgacgaccg
     3241 atctgttctt tacgctacac cttctcgatt gcttccccga cctgcgcctg accggcgata
     3301 tctcgcacta cgtcgtggga cgcgaattcg cctggccgat ctcggaggag aaccatcgcc
     3361 tgatgcatcg catcctcgac aacacctggg gatttcacgg gcgcgttgcg agccgcgagc
     3421 aggtgcagat ccagatcagc ttcccgcacc acaaggactg ggtggactta tttctgggtt
     3481 ggtgggagta cggcttccgc agctggcgca agcgtgcgcc gcgggacgcg acactcacct
     3541 tcctgtgcga gttgggcccg aaggaatatg cgatgaccgg gccggatggc tacgagcttt
     3601 ccgaccgctg ggaggaagcg aagatgatga aggacatggt gcgcactctc tggcggcggt
     3661 tggaagaaga cgagcgcgcc ccggcgacac gggctgcttg aacgcttccg cctttgtgca
     3721 ccgggcggct attgctgcgc tgattcttat ctcgtcgctg tcaccccgca gcgcaattca
     3781 ggcagcggaa cccgcgtggc ccccgtttct gcctcgctcc gtagattctg cgagcgaaaa
     3841 ggctgtggag agaatatgga ataagagaac tttcgagcgc aggctgaatc ccaacccggt
     3901 cgatgttccc attgacctgt acgcggccct gatctctacg ccggaagttg tcgccgcagc
     3961 cgccaaccgg ctggggttga cctcttctac cgttaccgtc gtggatgatc ggacttacga
     4021 atggcgaacc gcggagggtt cgcgggcgct ctaccggata ttgctgagcg aacctaggca
     4081 tcaggtgacg ctgttccagg gacacctgat catgtacggg ctgacagtgc acggttccat
     4141 gctcggtaac cttgatctgt cgaacgactc gaccggcgtg caccaagatc tgtccgtcta
     4201 cgtccaggtc gaccgctcga tcatggcgtg gctgacaaag ctgttcgtgg cgctgatacc
     4261 cgggaagatc gacgcggaag tgtcccacgg tttcgagttc actggcgcgg tagctcgctg
     4321 ggcttggcga gaccgggaag gtttctgtcg gtggcggcaa acatcggcat tcgcgcccga
     4381 gcggactgct cggattgccg agttggtcgg gtgcgcgacc gtggtgcatt gaacgaggtg
     4441 ctcgaattca aaccccatga catcgaacac gatgccgcct cgtcgagagt gaatctctgg
     4501 aagactcaag agcttccact aattcacgga ttgcgcgcgg tcacgatcca cgtgctcgaa
     4561 ggcgcccaca ccccgttccc tctggtgtag tgcgaaagcg tcttgcgcag tgcggccgcc
     4621 acctgatcct tgcgtttggc gccttcttct ccggcgaggc ggatgatctc gccggccgga
     4681 cccagcgcca tggcgaactc gatcgcctcg tccatgtctt tcccgatgca gatgtcggcg
     4741 tcgaagcgct cgaaggcgat ccggtcataa ccgctgccgc gaagcatcga gctcaccatg
     4801 tccgggcctg ccatcgaaaa cggccccggc ccgcagtgca cttcgtccgt gtcctcgtgc
     4861 gagacgaccg ggacgatttc cttcacgcac agctccgcat cgtggaccca gggattctct
     4921 tcgcgacgcc gccatacgat catggtcaac gctccaccgg gcttgagtgc cttgcgaatg
     4981 ttgcgcaggg ccgcgccagg cagcatgaag aacatcgttc cgaagcggga gaacgcgaag
     5041 tcgtaggggc cgcgaagatc gtcggcctgg acgtctgcca cgaagaacga ggcgttgccg
     5101 actccagtct ctctggattc ttgctgggcg ctcttgatga agttctcggc gcagtcgacg
     5161 cccaccgctt cgcctttggg tcccacgcgc ttggcgatga gacgagtgct gtcgccgaag
     5221 ccgcagccga cgtcgagcac gcgcgagccc tctggatagc cgccccggct cagcgcctca
     5281 ttactgtggg cagacaggcc gtcgatgagc agatgcttga agcggcaaaa cttgtcgtac
     5341 agaacggtgt tccacgcctc gacgacaacg gcgttatcgt cactcattga tgcaccttgt
     5401 ctgcaatatc atgtgtaccg gcgtgcagga atcgctctca tagaggagat tatagggtga
     5461 gcggtgagcg gatgcagtgg cgggtcaaaa tcgggactga cgagactgct gcggtcttcg
     5521 aagccgcttc cacggggagc aggggaagac tgttcgtctg tgcccacggc gccggcggtc
     5581 acatggcgga tcgcggcatg cttgcgctct ccgaagtgtt tcgcgggcgc ggaaccgatg
     5641 tcgtccgctt caattttctc tatcgggaaa aaggctcgag caaacccgac gcgatgccac
     5701 gcctggagga atgcgtttcg gcggtcgtgt cacgggcgcg ccaggaagtg cagcccgatc
     5761 tgcttttcat cggaggaaga tcgatgggcg ggagagcagc ttcgatgctg gcggccgatg
     5821 ggttttcgtg cgatgggctc atcctgctcg cctacccgct ccatctggcc gggcagccag
     5881 agaagctccg cagtgcgcac ctggtgcaga tcaaagtccc ggtcctaagc ctgaatggca
     5941 cccgcgacag cctctgcacg cgcgagctca tggatcaagt gaccggcgga ctgtccagcg
     6001 tctggaccat gcactggctg gaaggagccg accattcctt ccacgtattg aagagctcag
     6061 gcagatcgga tggcgatgtc ctg
//
LOCUS       DASPUL010000373         4662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_477172, whole genome shotgun sequence.
ACCESSION   DASPUL010000373 DASPUL010000000
VERSION     DASPUL010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477172"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VEJ00_09075"
     CDS             <1..504
                     /locus_tag="VEJ00_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXX01352.1"
                     /translation="GGNIRLGTERVRDAHVEQAAEDVNLADFIRALPNGFNEEVRERG
                     STLSTGQKQLISFARALAHDPKILILDEATSSVDTETEFRVRDALARMVEGRTSLIIA
                     HRLSTIQRADKILVMHKGQLREMGTHQELLAHRGIYYKLYQLQYKDQEIPLGSSQEPQ
                     STVSADD"
     gene            complement(543..2276)
                     /locus_tag="VEJ00_09080"
     CDS             complement(543..2276)
                     /locus_tag="VEJ00_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01353.1"
                     /translation="MLANRAPLATCGLALLIYLPSLFCGFIRDDYPQIVNNRQVQSWE
                     YLPQLLGSHLWSQIARDETILFYRPIFSVWMLLLHTTGGLEPWFWHLSNLLLHAVATY
                     LVYRFCRRLTGSDAGAVAAAAIFAVHPIHVEAVTWVSASCEILCTIFALVAMLTLLHE
                     NGDNDDSKNVNRQVWISALWFGAALFAKETGIAMLAILPVLAWVRLRDMVAGKERFWK
                     AGYPYGAVAACYLLIRWAVMRRMGMEIGEHSWAEVIFSGPSILLYYWKKLFVPLELSG
                     CYVTPLTGSPTPLFWLQVAGILIFLAATAWLAFRYRPLLALAAALIVIPVLLALAVIR
                     VYPQGDMTHDRYLYLPSIGLSLLVAMLVEDLWSRGRSARTAVMAVGVAVVAAFSALTF
                     TQQKYYHDDMAFCLRVIAVSPSDAFAHGQLGNLYLDQGRVDVALEQFWTGHQLDPGNE
                     KVTLFLARGLFVAGQYKNSETVLRELLQTKGLSTRRRNSAQISLANVEISLGNLSDAR
                     QLLEQVERRDDRFPELHWAWGVLYQKQGMLPQAQAEFEKEFEVTGDLQAQQRSALLVR
                     LIYSQSKGQVH"
     gene            2523..3740
                     /gene="lpxB"
                     /locus_tag="VEJ00_09085"
     CDS             2523..3740
                     /gene="lpxB"
                     /locus_tag="VEJ00_09085"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF014714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HXX01354.1"
                     /translation="MQILVSAGEASGEMYGAQLIEALRRAAEKKAVEPGSAASPGPAQ
                     LKRPATVSTGEISFFGVGGDRMRAAGCETVVDASDLSVVGITEILSHLPKIYGLYRHL
                     IREADKRHPDLAIVIDSPAFNWRVAREMKGRGVQTVYYVAPQFWAWRQGRVRLIRDYI
                     DKALVIFPFEEKFYRDRGVDATFVGHPLAELPQPAMGRDEYAARFNLDPAKHWITLMP
                     GSRMKEVRMNMPATLESAAKLGSGYEFLLPVAPTLERGWVAKLVDTGRITFVPESLPA
                     LHHSRAGIIASGTATVEAAMMNTPFVMVYRVSPLTYLMGRPRVKIPHFAMVNLIAGRE
                     VVPELVQSDFTASNVIVRLNEILPDGPVREQMLQGFAQVKARLRAPAGSGEKTRHPAD
                     RAAEIILSLVPGR"
     gene            3865..>4662
                     /locus_tag="VEJ00_09090"
     CDS             3865..>4662
                     /locus_tag="VEJ00_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01355.1"
                     /translation="MQIDRRRVRRALFTFLTLSTAVLFVLPSWAAEKARLRVDDYQIQ
                     AELEPHLHQITAHAKVRFTALQDLSIAVFELHNDLRVTKVLDEKNQPLSAERQTQDST
                     VRVPLPKGLAKDASTTLTFEYEGTLENADNSPVPGLKLAYIGDDTSYLLYAGRWFPVS
                     GYGLNRFTSTISVTLPAHMLAIGSGDVTVADNAPSKKPNASILPTKTFTFVSSKPSFP
                     GTIVAGIFQEYKSSEAGMDLHVFFKPTHQSLAPEYTTSAVQEFTYFIT"
BASE COUNT          990 a         1432 c         1344 g          896 t
ORIGIN      
        1 gggggcaaca tccgcctggg cacggagcgt gttcgggacg ctcacgtcga gcaagctgcc
       61 gaagacgtga acctcgccga tttcattcgc gcgctgccca acggattcaa tgaagaagtg
      121 cgcgagcgcg gttcgacgct ctcgaccggc cagaagcagc tcatttcttt cgctcgcgcc
      181 ctcgcacacg acccaaagat cctgatcctc gatgaagcga cctcgagtgt cgatacggaa
      241 accgaattcc gcgtgcgcga tgcgttggct cgtatggtcg aaggacggac ctcactgatc
      301 attgcgcacc gcctgtcgac catccagcgc gccgacaaaa tcctggtcat gcacaaaggc
      361 cagctgcgcg aaatgggcac gcaccaggaa ctgctcgccc accgcggcat ctactacaag
      421 ctctaccagc tgcaatacaa ggatcaggag attcccctgg gaagttcgca ggagccgcaa
      481 tcgacggtga gtgcggacga ttgacgcttg gttctgactc cgggctttcc cttcacctgc
      541 gtctaatgga cttgtccctt tgactgggaa tagatcagtc ttaccaagag cgctgatcgt
      601 tgctgagcct gcaggtctcc cgtgacctcg aattcctttt cgaactcagc ctgagcctgc
      661 gggagcatac cctgcttctg gtagagcact ccccaggccc agtggagttc gggaaagcgg
      721 tcatcacgcc gctcgacctg ttcgagcagt tgccgtgcgt cggagagatt tccgaggctg
      781 atttccacgt tggccaacga gatctgtgcc gagtttcgcc gtctggtgct cagtcctttc
      841 gtctgtaaca gctcgcgcag caccgtttcc gagttcttgt attgtccggc aacgaagagt
      901 ccgcgagcca gaaacagcgt gaccttctcg ttaccgggat cgagttgatg tccagtccag
      961 aattgctcga gcgcaacgtc aacgcgcccc tggtcgaggt agaggttgcc gagctgtccg
     1021 tgggcaaagg cgtccgaggg gctgacggcg atgactctta agcaaaaggc catgtcgtcg
     1081 tggtagtact tctgctgcgt aaaggtcaga gccgagaacg cggccacgac ggccacccca
     1141 actgccatca ccgcggtccg agccgaccgt ccacgagacc ataggtcttc caccagcatg
     1201 gcaacgagca gggaaagccc gatcgacggg aggtagagat agcggtcgtg cgtcatgtcg
     1261 ccttgcgggt agacgcggat tactgccaac gccaaaagca cgggaatgac gatcaaagcg
     1321 gcagcaaggg ccagaagagg acggtaacga aacgcaagcc aagccgtcgc ggcaaggaaa
     1381 atcagaattc cagctacctg cagccagaac aaaggggtag gcgagcccgt cagcggggtc
     1441 acgtagcatc cgctgagctc taacggaaca aacaatttct tccaataata gagcaggatc
     1501 gacggtccgc tgaagatgac ctccgcccag ctgtgctcgc caatctccat tcccatgcgg
     1561 cgcattacgg cccaccggat cagcagatag caggcggcaa ctgcgccgta aggatagcca
     1621 gccttccaaa accgctcttt ccccgcgacc atgtctctaa ggcggaccca ggcgagcacg
     1681 ggcaggatgg ccagcatggc gattccggtt tccttggcaa acagcgcggc gccaaaccac
     1741 agagcgctga tccatacctg ccgatttacg tttttgctgt cgtcgttgtc gccgttttcg
     1801 tggagcagcg tcagcatggc caccagcgca aagatcgtgc acagaatttc gcagctggcc
     1861 gagacccaag tcacggcctc gacgtgaatg ggatggacgg caaaaatggc tgcggcggcg
     1921 acggctcccg cgtcgctccc agtcagacgg cggcaaaacc ggtacaccag gtacgtcgcc
     1981 accgcgtgca gcagcagatt ggagagatgc cagaaccagg gctcgagtcc tccggtggtg
     2041 tgcagcagca gcatccagac cgaaaagatc gggcgataga agagaatggt ttcgtcccgc
     2101 gctatctggc tccaaagatg cgacccgaga agttgcggca agtactccca ggattgcacc
     2161 tggcgattgt tgacaatttg cgggtaatcg tcccggatga aaccgcagaa caaagagggt
     2221 aagtaaatga gaagcgcgag gccgcaggtg gccaggggtg cgcggtttgc aagcaagctc
     2281 tcgcgccaga ctgctcgtgc ggaaagacgc ccagcggatt cgcttcgctt tgcttttttc
     2341 atcgtcggct accaccacac cgtgaccact aattcaccgc gccgctccga catgcgctac
     2401 agactccgca ccagagtaaa tggcctaggc ctgtgggttc caaccgaccg cgctaccaac
     2461 cgagggcccg ttaggcagcc agaaatcccc agcatcgcaa tctgctccct tacaatgtcc
     2521 gcgtgcagat tctagtctct gccggcgaag cttccgggga aatgtatggt gcgcagctta
     2581 ttgaagcctt gcgccgggct gcagagaaga aggccgtaga gcccggctcc gcagcctcgc
     2641 ccggcccggc ccagctcaag cggccggcca cagtctccac aggggaaatc tccttcttcg
     2701 gtgttggcgg agatcgcatg cgggccgccg gatgcgaaac cgttgtcgat gccagcgatc
     2761 tctcggtagt cggtattacg gagattctca gccacctgcc aaaaatctac gggctatacc
     2821 ggcatctcat ccgcgaagcc gacaagcgcc accccgacct tgccatcgtg attgactctc
     2881 ctgcgttcaa ctggcgggtc gcgcgcgaga tgaaggggcg cggggtccag accgtctact
     2941 acgtcgcgcc ccagttctgg gcctggcgcc aaggacgcgt tcgcctgatt cgtgactata
     3001 tcgacaaggc tctcgtgatt ttccccttcg aagagaagtt ttaccgcgac cggggggtcg
     3061 acgcgacgtt tgttggccat cccttggccg agctgcctca accggcgatg gggcgggacg
     3121 agtatgctgc ccggttcaat cttgatcccg ccaagcactg gatcacgctc atgcccggca
     3181 gccgcatgaa ggaagttcgc atgaacatgc ctgcgaccct ggaatcggcg gctaagcttg
     3241 gctcaggcta tgaattcctg ctcccggttg ccccaaccct cgaacgcggc tgggttgcga
     3301 agttggtgga cacaggcaga atcactttcg ttcccgaaag ccttccggcg ctccatcatt
     3361 cgcgcgccgg aatcatcgcc agtggcaccg ctacggtcga ggcggcgatg atgaacacgc
     3421 cttttgtcat ggtttaccgg gtctcgccgc tcacttatct catgggcagg ccgcgcgtga
     3481 aaatcccgca ctttgccatg gtgaatttga tcgctgggcg cgaggtggtc ccggagctgg
     3541 tgcagagcga tttcacggcc tctaacgtga tcgttcggct caacgagatc cttcccgacg
     3601 ggccagtccg cgagcaaatg ctgcaggggt ttgcccaggt gaaggcgcgg ctgcgcgctc
     3661 cggccgggtc aggggagaaa acccgccatc cggccgaccg cgcggctgag ataatcctgt
     3721 ctctcgtgcc cggacgttga tgacgcttcc ccgaaacgtg acaaaatcct gacaaaccgc
     3781 ccctaaacct tgtgctacca gcatcttttg cgtcagaatc ttcatcatag gaaatgggtt
     3841 atcatttcct ggagactctc ctgaatgcag attgaccggc ggcgcgttcg gcgcgcccta
     3901 ttcaccttcc tgactctgtc aacagccgtt ttgtttgtgc tcccctcgtg ggctgcggag
     3961 aaagctcgtc tgcgcgtgga cgattaccag attcaggccg aactcgaacc gcatcttcat
     4021 caaatcactg cccacgccaa ggtccgattc accgcgctgc aggatctgag catcgccgtg
     4081 ttcgaattgc acaatgactt gcgggtcacg aaggtacttg acgaaaagaa tcagccgctc
     4141 tccgcggagc gacaaaccca ggactccacg gtgcgagtgc cgctgccaaa gggactggcc
     4201 aaagacgcct cgaccactct gactttcgaa tacgaaggca cgctggagaa tgcggacaac
     4261 agtccggttc ccgggctcaa actggcctat atcggcgacg acaccagcta cctgctctat
     4321 gccgggcgct ggtttcctgt cagcgggtac ggcctgaacc gcttcacctc gaccatcagc
     4381 gtcacgctgc cggcgcacat gctggccatt ggcagcggag atgttacggt cgccgacaac
     4441 gctccgtcaa agaagccgaa tgcgagcatt ctgcccacga agacatttac attcgtttcc
     4501 agcaagccaa gcttccccgg cactatcgtc gcgggcattt ttcaggagta caaaagcagc
     4561 gaagcgggga tggacctgca cgtctttttc aagccgacgc atcagagcct tgcgccggag
     4621 tacaccacgt cggcggtgca ggaattcact tacttcatca cg
//
LOCUS       DASPUL010000374         4818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1714331, whole genome shotgun sequence.
ACCESSION   DASPUL010000374 DASPUL010000000
VERSION     DASPUL010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1714331"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..941
                     /locus_tag="VEJ00_09095"
     CDS             141..941
                     /locus_tag="VEJ00_09095"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HXX01356.1"
                     /translation="MSEHEQVAEDLALHALNALTGEERANLERHLATCVSCRAELEHL
                     RGDGALLALSTMGPKPPLRARQRLLDAVAKEVPTSRAQNAREMGHPAVSNQRRSWWGW
                     LGWAATAAVVVFATSLWKENAGLRENLAVARATVEGNAKQLEDLRRVAAPILEPDAQR
                     ITLVSVKTPPQPQGKAFYLRNRSSLLFVANNMPPLPPQKVYELWLIPVQGAPVPAGVF
                     KPDRHGSATVVNPPLPAGMEAKAFAITVENEAGASTPTMPIVMMGTGE"
     gene            995..1630
                     /locus_tag="VEJ00_09100"
     CDS             995..1630
                     /locus_tag="VEJ00_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HXX01357.1"
                     /translation="MTFPSHTVRLLPLGLLLVLSSAVRGGPAQEMPASEQHSIAVKLK
                     AKGIPNFGQVSSTLYRGAQPTSEGFETLKGTGIDIVVDLRGGNSKSEKAAITKLGMQY
                     VSIPSHCPFPSDKPWAEFLKMIEDNPGKKVFVHCRLGDDRTGMAVASYRMAEEGWSAK
                     EAMKEMKTFGFSAMHHAICPGLEEYEEKFPQRLKKSPAFRDLAVRKAQPAQ"
     gene            1753..1938
                     /locus_tag="VEJ00_09105"
     CDS             1753..1938
                     /locus_tag="VEJ00_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01358.1"
                     /translation="MGLLEEAEKIAGAVAAVEGIKKLDPNAGLVTEAAAAITGFEGAG
                     AIAERLEKKEDDSDKQG"
     gene            complement(2107..3405)
                     /gene="murA"
                     /locus_tag="VEJ00_09110"
     CDS             complement(2107..3405)
                     /gene="murA"
                     /locus_tag="VEJ00_09110"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXX01359.1"
                     /translation="MDKFVIRGGEPLLGTVRVSGAKNAALPCMAAALLTDQPVILENI
                     PQVRDIQTTRNLLAAMGAEVELGYGRAQHRTSIHCATLTAPEASYELVKTMRASTLVL
                     GPLVARCGRARVSLPGGCAIGARPIDLHIKGLEQLGAKITQEHGYVEATATRLKGAEV
                     VFDKITVTGTEDLLMAATLAEGETILQNCAREPEVADLADLLNKMGAKIEGTGTPTIR
                     VRGVSKLKGAKHRIIPDRIEAGTFLIAGAMTGGDLNVAGCDPSHLGAVIGKLHEVGVK
                     TKSSADSVRVMGDNPFTAADLSTEEYPGFPTDCQAQFMALATQAEGMSIITENIFENR
                     FMHALELVRMGANIKIEGRRAVVRGKTPLSAAAVLASDLRASASLVLAALVADGETII
                     DRVYHIDRGYEHIEEKLKAVGAQIRRIGEMFPRKATPAKA"
     gene            complement(3575..4087)
                     /locus_tag="VEJ00_09115"
     CDS             complement(3575..4087)
                     /locus_tag="VEJ00_09115"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXX01360.1"
                     /translation="MLSRQLFHVLFALTIVLGSVHLAAQNADQPIVNPAAAAKFGPVP
                     NTPACFTIAVEKGDPTKGPSVILAKFAPGCVAPFHWHTPSETAMIVSGSLETQMKGDK
                     AIVGHRGDFVFLPARHVHRATCQGAVPCLVFLTSDAAFDIHWVNADGKEISLEEAMKA
                     VKAPVKQGQM"
     gene            complement(4179..>4818)
                     /locus_tag="VEJ00_09120"
     CDS             complement(4179..>4818)
                     /locus_tag="VEJ00_09120"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA];
                     GO_process: GO:0097173 - N-acetylmuramic acid catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HXX01361.1"
                     /translation="NTASRTVQFVMAGGPRALGASTEASEDDTRSAVAEMKKRKPGKN
                     DVVLGIASSGRTPFTIATIEYARRRGARTIALTCNRDSPLERAAGFAIVTEVGPEVLA
                     GSSRMKAGTAHKMVLNMISTAAMTRLGYVYGNLMVNVWTKNEKLIRRAIRILQEATGV
                     DAETAARALKAAGNRTPVALVMLAANVTRSKAATALKKSKGGVRQAIELARS"
BASE COUNT          952 a         1339 c         1602 g          925 t
ORIGIN      
        1 caagacggga gaacccttgg gcacgattaa gacacgcatc cgggcggggc tgatggcgct
       61 gcggaaggcg tttcagacgt gaagaaccca cttctcgggc aaaaaacgcg cgagaaatgg
      121 ggcacccaaa ttcgataaag atgagtgagc acgaacaagt cgcggaggac ctggcgctgc
      181 atgcgttgaa cgcgctcacc ggtgaggagc gcgcgaacct tgagcgtcac ctggccacgt
      241 gtgtttcgtg ccgcgccgag ctcgaacacc tgcgcggcga cggcgcgctg ctggcgctgt
      301 cgacgatggg gccgaagcct ccgctgcggg cgcggcagcg gcttttggat gccgtggcga
      361 aagaagtccc cacttctcgc gcacaaaacg cgcgagaaat ggggcacccc gctgtatcaa
      421 accagcggcg ctcgtggtgg ggatggctag gctgggcggc gacggcggcg gttgtcgtat
      481 tcgctacgtc gttatggaag gaaaatgccg ggctcaggga gaacctggca gtagcgcggg
      541 ccacggtcga agggaacgcc aagcagctgg aggatctgcg ccgtgttgcg gcaccgatcc
      601 tcgaaccgga cgcacagcgc atcaccctgg tgtcggtgaa gaccccgccg cagccacagg
      661 gcaaggcttt ctatctgcgg aaccggagca gcctgctttt tgtggcgaat aatatgcccc
      721 cgctgccgcc gcagaaggtg tacgaattgt ggctgattcc ggtgcaaggg gcaccggtcc
      781 cggcgggtgt gttcaagccg gacaggcacg gcagcgcgac ggtggtgaat cctccgcttc
      841 cagcagggat ggaggcgaag gcatttgcca ttacggtgga gaacgaagcg ggcgcttcga
      901 cgccaacgat gccgattgtg atgatgggga cgggtgagta gttctcgctg cgcactgctc
      961 ggtgcatccg ctaaaatctg atcatcgccc gtcaatgaca ttcccatctc ataccgtgcg
     1021 gctcctgccg cttggactgc tgctggtcct gtcatccgcg gtccgcggag gtccagcgca
     1081 ggagatgcct gcttcggagc aacattcgat tgcagtcaaa ctgaaggcga aagggatccc
     1141 caacttcggg caagtcagct cgacgctgta tcgcggggcg cagccaacca gcgagggttt
     1201 cgaaacgctc aagggtaccg gaatcgatat cgtggttgat ctgcggggcg gaaacagcaa
     1261 gagcgaaaag gccgcgataa ccaagctggg gatgcagtac gtttctattc cttcgcattg
     1321 tccgtttccc agcgataagc cctgggccga gtttctgaag atgatcgaag ataatcccgg
     1381 taagaaagtc ttcgtgcact gccggttggg cgacgatcgc accgggatgg cagtggcgtc
     1441 ctatcgcatg gcggaggaag gctggtcggc gaaagaagcg atgaaagaga tgaagacctt
     1501 tgggttcagc gccatgcatc atgcgatttg tccggggctc gaggagtatg aggagaagtt
     1561 cccgcagcgt ctgaagaaga gccctgcgtt tcgcgacctg gcagtacgca aagcacaacc
     1621 cgcgcagtga gcacgggggt tgacctagtg attgaaatcc gctcttgaca cccgctaccg
     1681 ctcagcatac aagtgttgct tcgaattgcg cccgttgagg cgtgaaatat attccgggta
     1741 aggagactga tcatgggact cttggaagaa gcggaaaaga tcgcaggagc ggttgccgcg
     1801 gtggaaggca tcaagaaact ggatccaaac gccgggctgg tgacggaggc ggcggctgcc
     1861 atcacgggat tcgaaggggc aggcgcaatt gccgagcgcc tcgagaaaaa ggaagacgac
     1921 agcgacaagc agggctaggg caccatcgct gttcttcgtg gtgaaggcct ggcgctgaca
     1981 gggatcttct cagcagctga aggcccgatt cattgaaggc gcgcctgacg cggcgctgaa
     2041 gcaccgctct tccacggcga cgtgcgggcc cgtgctgtgt cttcgagttt gcgcgcgccc
     2101 ggttcgctat gctttggcgg gagttgcctt cctgggaaac atctcgccga tgcggcggat
     2161 ctgagctccg acagccttca atttctcttc aatgtgctca tagcctcggt cgatgtggta
     2221 gacgcggtcg atgatcgtct ccccgtcggc aacgagcgca gccagcacca gcgacgccga
     2281 ggctcgcagg tcgctggcca gtacggcggc ggcgctaagc ggagtcttgc cgcgaacaac
     2341 agcacggcgg ccttcgatct tgatgttcgc gcccatgcgc accagttcga gtgcgtgcat
     2401 aaaacgattc tcgaaaatat tttccgtgat gattgacatg ccttcggcct gagtagcgag
     2461 ggccatgaac tgcgcctgac agtcggtggg gaagccggga tactcttccg tgctcagatc
     2521 ggcggcagtg aaaggattgt cgcccatcac gcgcaccgag tcggcggacg atttcgtctt
     2581 gaccccgact tcgtgcaact tgccgatcac ggcgccgaga tgcgatgggt cgcagcccgc
     2641 aacgttcaga tcgcctccgg tcatggcccc ggcaatgagg aacgttccag cctcgatgcg
     2701 gtcgggaatg atacggtgct tcgcgccttt cagcttgctc acacctctga cgcggatcgt
     2761 aggcgtgcct gttccttcga tcttcgctcc catcttgttg agcaggtcgg cgaggtcggc
     2821 gacttcaggc tcgcgcgcgc agttctgcag gatcgtctcg ccttcggcga gcgttgcggc
     2881 catgagcaag tcttcggtgc cggtgactgt gatcttgtcg aaaacgactt ccgcgccttt
     2941 gaggcgagta gctgtggctt cgacgtagcc gtgttcctgc gtgatctttg ctccgagttg
     3001 ctcaagtccc ttgatatgca gatcgatggg gcgggcgccg atggcgcagc ctccggggag
     3061 cgaaacgcgg gcgcggccgc agcgcgcgac caatggtccg aggacgagcg tggacgcgcg
     3121 catggtcttg accagttcgt aggaagcttc gggcgcagtc agcgtggcac agtggatcga
     3181 ggtgcggtgc tgggcgcgtc catagccaag ttcgacttcg gcacccatgg cggcgagcag
     3241 gttgcgcgtg gtctgaatat cacgcacctg cggaatattt tcgaggatga cgggctgatc
     3301 ggtgagcagg gcggctgcca tacaggggag cgcggcgttc ttggcgccgc tgacgcggac
     3361 cgtgccgagc agcggctctc cgccgcgaat gacgaacttg tccatgaaaa tggttcctgg
     3421 attgattgta acggggttcg tgcgagcgaa ggggtgactg aagcggaacg ggaggacaag
     3481 gactgcggcg caaatcctcc gccgcaggaa cagtggaaca cagccgccgg ggaaacccac
     3541 ccttcggaag atcgcgaaag gtgggcgagc ggcgttacat ctggccttgc ttaacagggg
     3601 ccttgacagc cttcatggcc tcctcaagcg aaatctcctt gccgtcagca ttcacccagt
     3661 gaatatcaaa tgctgcgtcg gacgtgagga agaccaggca gggaactgct ccctggcatg
     3721 tggcgcgatg cacgtgacgc gcgggaagaa acacgaagtc gccacgatgg ccgacgatag
     3781 ccttatcccc tttcatctgc gtctccaggg agccgctgac gatcatcgcc gtttcgctgg
     3841 gcgtatgcca gtgaaaggga gcaacgcatc cgggagcaaa cttggcaagg atgaccgagg
     3901 ggcccttggt cggatccccc ttctcgacgg cgatggtgaa gcatgcggga gtgttcggta
     3961 ctgggccgaa cttggcggcc gcggcaggat tcacgatggg ttggtcggca ttctgtgcgg
     4021 cgagatgaac gctgcccagg acaattgtca gagcgaaaag cacgtggaag agttggcggg
     4081 aaagcatgat cctcctttcg actgcgactc gaaggggccc caaagatcga agcgtacttt
     4141 aatcaaaatg cgagtgaaag taaactgcgg cggcatggct atgaccgagc cagttcgatc
     4201 gcctggcgga cgcctccctt gctcttcttc agcgcggtcg ctgctttgga tcgagttaca
     4261 ttcgccgcca gcatcacgag cgctaccgga gtgcggttgc cagcggcttt tagggcacgg
     4321 gcggcggttt ccgcatcgac ccccgtggct tcttggagga tgcggatggc gcgccggatg
     4381 agtttttcgt tcttggtcca aacattgacc atgagattgc cgtagacgta gccgaggcga
     4441 gtcatcgcag ccgtcgagat catattcaaa accattttgt gggcggtgcc ggccttcatg
     4501 cgagaagacc cggcgaggac ctcaggaccc acttcggtga cgatcgcgaa gccagcggca
     4561 cgttccagcg gtgaatcgcg gttacaggtg agggcgatgg tgcgggcgcc gcggcgtcgg
     4621 gcgtattcga tcgtggcaat ggtgaagggc gtgcggccgc tggaagcgat gccgagtacc
     4681 acatcgttct tgcccggctt gcgcttcttc atctcggcga ctgccgatcg agtgtcgtct
     4741 tcgctggcct cggtggaagc gcccagggct cttgggcctc cagccataac gaattgcacg
     4801 gtccgactag cggtattg
//
LOCUS       DASPUL010000375         4093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1292318, whole genome shotgun sequence.
ACCESSION   DASPUL010000375 DASPUL010000000
VERSION     DASPUL010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292318"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VEJ00_09125"
     CDS             <1..958
                     /locus_tag="VEJ00_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HXX01362.1"
                     /translation="RDPSFEITAVASASDVLPAAVTRRADVALISSDIDNVSRKGLEV
                     ARTLSAHRPDVRIVILIEANTREIVVASFRCGATGVFWRSEPLSELHSCIQRVSRGEI
                     WASSNQAQHLLETLRSTPSYDGLHEGKIGMLSKRELEVAECAVQGHSNRQIADHLQLS
                     EHTVKNYLFRVFEKLGVSSRFELLFLLFHENHASINNGPREEALRRGNPMDPYLKAAD
                     EGFVAAQFIVGLAHLEGYCVEKNSCSAYYWLRMAEENSSGLNKHTRSLVAELRATMKS
                     DEVQALERNVASAVRRQKVLAGKSPAELIRQNPGSPSWRIAV"
     gene            complement(1384..3612)
                     /locus_tag="VEJ00_09130"
     CDS             complement(1384..3612)
                     /locus_tag="VEJ00_09130"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXX01363.1"
                     /translation="MPLASGTKLGPYEVVRPLGAGGMGEVYRARDTRLGRDVALKILP
                     AEFSADPVRKQRFEQEAKTISSLNHPNICVLYDVGTQHGVDYLVMECVEGETLSQRLE
                     KGPLPVDQVLKYGAQLADALDKAHRGGIVHRDLKPGNIMLTATGAKLLDFGLAKSAVP
                     LVSATLTAAAPQPAPPTQVGTVVGTFQYMSPEQIDGEEIDGRSDIFSLGAVLYEMLSG
                     IPAFHGQTIPQVINEILRHNPPPSPVPALDPLLRRCLAKQAAQRFQTMKEVRAALERV
                     AQAAAGTTERSVAVLPFANRSADPEDECFSDGLADEIINALMRVPGLKVIARTSAFAF
                     KGKQDDIRRIAEALGVENVLEGSVRRAGNRVRITVQLITAADGTHLWSERYDRELADI
                     FAIQDDIAQAIATALRVTLAARPSDIQRHIPKLPAYEALLKGRYHMLRQVGRDERFKM
                     YFEQAMALDPDYAEPHANLGLAHFLSAMIGSQSMQKTMPLVRAEAQQALSLDPSDAGP
                     HFLLGSVAASYEYDWKRAAEHFTTAMAHRSSVSPETHWAYASFYLQPLGRFQEAVSEM
                     ERAVELDPLNVFWRGVLASHLTHAQLYDRAIEQAKEALEMDAASYVPHFTLGEAYATS
                     GQWTKAIEALENAHRVMPHDGLVSGMFAGALARTGQKSRAEGLIQQMGEAPRPIFGRV
                     FYHVLCGDTNAAADWYERAIDERDPFALVFASVPLTTDFRQSSRWPKLARMMNMPEQL
                     AT"
     gene            3774..3911
                     /locus_tag="VEJ00_09135"
     CDS             3774..3911
                     /locus_tag="VEJ00_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01364.1"
                     /translation="MEVGRSEALERNVASAVRRQKVLAGKSPAELITQNPGSPSWPIA
                     V"
BASE COUNT          843 a         1229 c         1172 g          849 t
ORIGIN      
        1 acgtgatccc agctttgaaa tcacggccgt tgcttcagca tcggacgtcc tgccggcagc
       61 tgttactcgc cgagctgacg tcgcactgat cagctcggat attgacaacg tgtccaggaa
      121 ggggcttgaa gtggctcgca cgttgagtgc gcaccgccct gacgttcgca tcgtaattct
      181 catcgaggcg aacactcgtg aaatagtcgt cgcttctttc cgttgcggcg cgaccggagt
      241 gttttggcgc agcgaacctc tgtcggagct tcattcgtgc atccagcgcg tcagccgggg
      301 ggagatctgg gccagcagca accaggctca acacctgctt gagacgctgc gcagcacgcc
      361 atcctatgac ggcctgcatg agggcaagat tggaatgctc tctaagcgtg aactggaagt
      421 tgccgagtgc gctgtccagg gccacagtaa ccgccagatc gccgaccatc tccagctcag
      481 cgaacatacg gtcaagaatt atctttttcg tgtgtttgag aaactcggtg tgtcgagtcg
      541 gttcgagctg cttttcttgc tgttccacga aaatcacgct tccatcaata atgggccaag
      601 ggaggaagca ctccggcgtg gtaatcccat ggacccctat ctcaaggcgg cggacgaggg
      661 attcgttgcc gcccaattca tagtcgggct ggcacacctc gaaggttatt gcgtggaaaa
      721 gaatagctgt tccgcgtact attggctgcg aatggcggaa gagaattcct cgggtctcaa
      781 caagcatact cgttctctgg tggcggagct cagagccacc atgaagtcgg acgaagtgca
      841 agctctggag cgcaacgtcg cctccgcggt ccgacgccag aaggttcttg ccggcaagag
      901 cccggcggag ctgatcaggc agaacccagg ttcaccatcc tggcgaatcg cagtgtaggg
      961 ccccttccca aggtcgtaac tcccccatgg aggtcagatt cgtgcttagc ccaacgaggc
     1021 cagaaaggcc cgacgatcca gagcgcagaa aagacgttgc ctgggagaat ctctgtcgcg
     1081 agagtggttg gacctgccgc atctgtggtg ctattccaga acgcggcaag cggttcgaga
     1141 aagagttgtg tgaggactgc cgactcttgg tcaagaacga atgagccgac gccccctatc
     1201 acgctgaatc ctgccgtaca gttaaggtcg tcgagcccgt gaccgagagg ctggaattgc
     1261 cgtctcgccc tgacgcgacg ggcggtagcc aggccggaac tgttgatggt cgccacagtc
     1321 cggattacag gcaaatgcct ttagggaaga gttaccgaca aatcccccga aagcagctcg
     1381 atctcaggtt gccagctgct cgggcatatt catcattctc gcgagcttcg gccaccgcga
     1441 cgattgtcga aaatcggtgg ttagcggcac gctcgcgaac acgagggcaa atggatcacg
     1501 ctcatcgata gcacgctcgt accagtccgc cgccgcattg gtatcgccgc acagcacgtg
     1561 atagaaaacc ctaccgaaga tcgggcgcgg cgcttctccc atttgttgaa tcaatccttc
     1621 tgcccgcgac ttctggccgg tgcgcgcaag tgctcccgca aacatgccgc taactaggcc
     1681 gtcgtgaggc atgactcggt gcgcgttctc gagagcctcg attgctttgg tccattgtcc
     1741 gcttgtagcg taggcctcgc ccaacgtgaa gtgggggaca tagctggccg catccatctc
     1801 cagcgcctct ttggcttgct caatcgcgcg atcgtacagc tgcgcgtgcg tcaggtggct
     1861 ggccaacacg ccccgccaga agacattgag cggatcaagt tcaacggccc gctccatctc
     1921 ggacactgct tcctgaaagc gccccaaagg ttgaaggtag aagcttgcgt atgcccagtg
     1981 cgtctccggc gaaacggatg atctatgcgc catcgcggtg gtaaagtgct cggctgccct
     2041 tttccagtcg tactcgtacg atgcggcgac agacccaagc aagaaatgag gaccggcgtc
     2101 ggacgggtca aggctcaagg cctgctgcgc ctcagcgcga accaagggca ttgtcttctg
     2161 catagattgg gagccgatca tggcggaaag aaagtgggcc aaacccaggt tggcgtgtgg
     2221 ttcggcatag tcgggatcca acgccattgc ctgctcaaaa tacattttga atcgctcgtc
     2281 gcggcccacc tgcctgagca tgtggtaccg ccctttgagt agcgcctcgt aagcaggtaa
     2341 tttcggtatg tggcgctgga tatcgctggg cctcgcagca agtgtgacgc gaagagctgt
     2401 tgcgatcgct tgcgcgatgt cgtcctgtat ggcgaagata tcggcgagct cccggtcgta
     2461 gcgctcggac cagagatggg tgccgtcggc agcggtaatc agctggacag tgatccggac
     2521 gcggttgccg gccctcctca cgctgccttc cagcacgttc tccacgccga gcgcttctgc
     2581 gatccggcga atgtcatcct gcttgccttt gaaggcgaaa gccgaggtgc gcgcaataac
     2641 cttcagaccc ggtacgcgca ttaacgcgtt aatgatttcg tcggcgagac cgtcgctgaa
     2701 gcattcgtcc tccggatccg cgctacgatt tgcgaacggc agcacggcaa ccgatcgttc
     2761 tgttgtaccg gccgcggctt gagctactcg ttccagcgca gcccgcacct ctttcatggt
     2821 ctgaaatcgt tgcgcggcct gcttcgccag acatcgcctg agaagagggt ctagtgcggg
     2881 caccggcgag ggtggcgggt tgtgacgtag aatttcgtta atcacttgtg ggatcgtttg
     2941 cccatgaaac gccggtatgc ctgaaagcat ctcgtacagc acagcgccaa gcgaaaaaat
     3001 atcgctgcgc ccgtcgattt cttcgccatc aatctgctct ggcgacatat actgaaacgt
     3061 gcccacgacc gttcctacct gcgttggagg cgctggctgc ggcgctgccg ctgtcagggt
     3121 ggcgctcacc aacggcactg ctgactttgc cagcccaaaa tccagcagct tcgccccagt
     3181 cgccgtcagc ataatgttgc ccggcttcaa atcccggtgc acgatgcccc ctcgatgcgc
     3241 tttgtccagc gcgtccgcaa gctgcgcccc atacttcagc acctgatcga ccggcagcgg
     3301 tcctttctcc agacgttggc tcagcgtctc gccttccacg cactccatca ccaaatagtc
     3361 cacgccatgc tgagtgccca catcatagag cacacaaatg ttagggtgat tcaggctgga
     3421 aattgtcttc gcttcctgct caaaccgttg cttccgcacc gggtctgcgg aaaattccgc
     3481 aggcaatatc ttcaacgcca catcccgtcc cagccgcgta tcccgtgccc gatacacctc
     3541 acccatcccg cccgctccaa gcggtcttac aacttcatat ggtccaagtt ttgtgcccga
     3601 agcgagaggc ataatggggg cgattatacg atgcaaaacc cttgcacgat aactccggtc
     3661 gagttcctgg acgtatcacg ggtaatcccg aaatcgctgt cctccgaccg cgatatgatc
     3721 tggaaattcc tcgggcgcgg actcgctctc aagttgccca gctccgagcc accatggaag
     3781 tcggacgaag tgaagctctg gagcgcaacg tcgcctccgc ggttcgacgc cagaaggttc
     3841 ttgccggcaa gagcccggcg gagctgatca cgcagaatcc aggttcacca tcctggccga
     3901 tcgcagtgta ggcccccttc ccaaggtcgt aactcccgct gcttagccca acgaggccaa
     3961 aaaggcccgg agcgcagaaa agacgttgcc tgggaaaatc tctgtcgcga gagtggttgg
     4021 acctgccgca tctgtggtgc tattccagaa cgcggcaagc ggttcgagaa agagttgtgt
     4081 gaggactgcc gac
//
LOCUS       DASPUL010000376         7371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_748402, whole genome shotgun sequence.
ACCESSION   DASPUL010000376 DASPUL010000000
VERSION     DASPUL010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748402"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1782)
                     /locus_tag="VEJ00_09140"
     CDS             complement(<1..1782)
                     /locus_tag="VEJ00_09140"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXX01365.1"
                     /translation="MKPILTLAGIALACLVAAQSFAQVQPNGDVLGMHNLSLASGASA
                     YTQGSLGCTFCHAPHSGLGANTPLWNQKLSTKSYVPYSSSTNPNQGNTQPTLGVTSSL
                     CLSCHDGTVGVGNSAVYGQIPMVGSLNAADSFGTTLTGSHPFSMVLPMKDASNLVATL
                     VSGGKTADPTGAVKLINGNVECTSCHDPHVQNTDRIAENFLVRDSSSGQMCLACHDPN
                     RVVQGQVNMLAGFTNSIHATATNQVSSDAHAGPYPTVGVNACSSCHMEHNSQAPMRLL
                     RPAVPAAGSYDSVTQSCMTCHGGGTYLSPAIPNIMAEVAKVSHPLPSGNNFHDAAEPA
                     VLNNNRHATCVDCHNAHAGNQEVQFSSPPAIRPPQQGATGVSALDGITVLNPAINQYE
                     TCLRCHGTSIGKQRLIIYGYAPVRLVSAADALNVIAEFSSSSTSSHPVTHPRSSALPQ
                     PSLLVNMLDLKGLPSARVVGTQIFCSDCHNSDDNREFGGNGPNGPHGSVYSHLLERNY
                     QFSQAATPGGPVTNTFPTPDTSVAGPYALCAKCHNLTQIFANTSFTQHNLHSYQLGFT
                     CSVCHTAHGMGANSPNVTGERMVNFDAN"
     gene            complement(1779..2879)
                     /locus_tag="VEJ00_09145"
     CDS             complement(1779..2879)
                     /locus_tag="VEJ00_09145"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HXX01366.1"
                     /translation="MVRLLLGRSAGPVRIGLAAGLLLVTLLPARAAKHAKETDQLVAP
                     DLLMDGGRKLSFERAFHLEREVKPKRGFWNRLVDVIAGQPDYRYLASPYGVVTDSRGR
                     IIVTDVSAVGVHIFDFDQQKYKFISRRDVGKNPMLMPQCVAVDAQDNIYVTDSMSGKI
                     FVFDAGGKFRRVVGALKGGEGYFKRPTGIAVDSAAGRMYVSDTLRNKIFVMDMQGSVL
                     QTIGKTGTGDGEFNFPTELRLNGNDLAVVDAMNFRVQVFDRSGTFRYSIGKVGDGAGW
                     IFRPKGIGFDSEGHLYVVDGEWGLVQVFDEQGRLLYYFGGKGTGAEQFQLPTGLHIDN
                     HDRIYVVDSFNRRVQIFHYYGIGASADGRKQP"
     gene            complement(2861..4714)
                     /locus_tag="VEJ00_09150"
     CDS             complement(2861..4714)
                     /locus_tag="VEJ00_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01367.1"
                     /translation="MKFANRIIGVAVLVMAHLLYAQTPSQQKTPELSIGDNTKLSAGG
                     QATFGYSGDYGDAITGSHGLTWGFDGRVNGYYYNPNFLSFTATPYYNQSRNDSSYQSL
                     TGATGIDATANLFSGTHFPGSVSYRYDRNSTGTFGLPGQPSFTTIGKSQGFGIDWSAL
                     VPGLPTLAVGYSQGDGSGTLFGTSQDTTSNTKLFNVRSNYDIEGFRLNAFFHRNSLSS
                     KFPEFLSGQESSLEHSVGHDVGFGAQHALPFRGQFYVNFDRASADANYFSDAAENSNT
                     SSYTDDIVNAGASFHPTQKLSLNLTENYTSNLNGYLSQSLTSTGAPVQGLSLGAGAHS
                     STVGGGATYQFTRFLSGTTQATYYDQEYLGKSYTGTFLSGTVSYGKRLLDMFTFSGSV
                     VDTSNGQGTNALGFVGNVNFFHRIQGWQLAGVFSYAQNVQTLLVTYTTSYFQYSGNVH
                     RRVAPGIQWTAAFNGMHSGLTNYSGTTASSESYSTALGLRKLTLNGIYTHSTGVSLLG
                     AGGFVPVTATPGASSLITFGGDSYGGGLSVTPMRRLVLAGSYNRAISNTLGQTFSHNN
                     TEIYNAQLQYHLRRVGLQAGYTRFTQGISAAGLPANTTSYFAGISRWFDFF"
     gene            complement(4999..6036)
                     /locus_tag="VEJ00_09155"
     CDS             complement(4999..6036)
                     /locus_tag="VEJ00_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXX01368.1"
                     /translation="MRSRLTICSLFGLALMMASTHFAWTQVASHLPTTVANPASAQVP
                     PPQVTDKPVARVNGAVLTDRDLLREMYTIFPYAQQHNGFPKAQEAEIRRGALEMIIFE
                     ELVYQEALRRKLTIPAEKLSQAEADFRKQFNSPDQYQQYLQTEMKGSEQQLRQQIKRS
                     LLIEQVLKTDVELKSAVSPAEVEAYYAKNPARFQQPESFSFQSISIVPPLKATPEQEK
                     DARKKAEAALQQAKATKTYQDFGLLAEKISEDDFRVNMGDHRVVAADKLPPQVVKVLQ
                     ALQPGQITGLIQIESAFTIIRMNAHTPAHKRPLAEVRNDLKTELQKSKYEKLRSNLDK
                     QLRAKAKIEMG"
     gene            complement(6121..7041)
                     /locus_tag="VEJ00_09160"
     CDS             complement(6121..7041)
                     /locus_tag="VEJ00_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01369.1"
                     /translation="MRKAILIVMLVLAVGGLVMAQNWTSPVDVLGAHNNGGRGCAGCH
                     APHSGSFGSGHSGAADTGTYALWGQDASPLYGKTIAFGDNGNYAEVLPNGIATGSQEV
                     GGILLCLSCHDGNVTAKNMMVNQSYEQRIGLLTNTAYGNGAIPTLLGNDGSGAGNYTN
                     DHPVGQNANISTTMPGLTWSGTAFNPPTAGSQYAQFVANYGYPALAPGKWSNPYAVTA
                     GGKPFVLCTTCHNQHVMTVYTSSKNSPVAGDGGGKYYATYFFINGPYNPNINNVANTN
                     APSTTQFCRQCHFGESNEANNTNNITTVFQ"
BASE COUNT         1391 a         2160 c         2269 g         1551 t
ORIGIN      
        1 gttggcgtcg aaattcacca tgcgctcgcc ggtcacgttg ggagaattcg ctcccatgcc
       61 atgcgccgtg tggcacaccg aacacgtgaa ccccaactga taggaatgca gattgtgctg
      121 cgtgaagctg gtattggcaa agatctgcgt caggttgtgg cacttcgcgc acagcgcgta
      181 cggcccggcc acactcgtat ccggagtcgg gaaggtattc gtcaccggcc cgccgggcgt
      241 tgccgcctgg ctgaactgat agttccgttc gagcaggtgc gaataaacgg atccgtgcgg
      301 accgttgggt ccgtttccgc cgaactcgcg attgtcgtcg ctgttgtggc aatcgctgca
      361 gaaaatctgg gtccctacca cgcgcgccga cggcagtcct ttcagatcca gcatgttcac
      421 cagaaggctg ggctggggca gcgcgctcga gcgcggatga gtgaccggat ggctggaagt
      481 ggaagaggat gagaactccg caatcacgtt cagcgcatcc gcggcggaga ccagccggac
      541 cggtgcatat ccgtaaatga tgagacgctg cttgccgatg ctggtgccgt ggcagcgcag
      601 gcacgtctcg tattgattga tggcggggtt cagcaccgtg atgccgtcga gcgcgctgac
      661 gccggtggcg ccctgctgcg gcggtcgaat ggccggcggc gacgaaaact gtacttcctg
      721 gttgcctgcg tgcgcgttgt ggcaatccac gcaggtggcg tggcggttgt tgttgagtac
      781 agcgggctcg gcagcatcgt ggaagttgtt gccggagggc agcggatggc tgaccttcgc
      841 cacttcagcc atgatgttgg ggatcgccgg cgacaggtac gtgccgccgc cgtggcaggt
      901 catgcagctc tgggtcaccg aatcgtagga gcccgcagcg ggcacagcag gccgcagcag
      961 ccgcattggc gcctgcgaat tgtgctccat gtgacacgat gagcacgcgt tcactcccac
     1021 cgtcggatac ggcccggcgt gcgcatcgga cgaaacctga ttcgtcgcag tcgcgtggat
     1081 gctgttggtg aaacccgcca gcatattcac ttgcccctgc accacccggt tgggatcgtg
     1141 gcaggccagg cacatctgcc cgctggagct gtcgcgaacc agaaaattct cggcaattcg
     1201 gtcggtgttc tgcacatgcg gatcgtgaca gctggtgcac tcgacgttgc cgttgatcag
     1261 cttcaccgcc ccggtgggat cggccgtctt gccgcccgaa accagcgtcg ccaccagatt
     1321 ggaggcgtct ttcatcggca gtaccatgct gaagggatgc gagccggtca gcgtcgtccc
     1381 gaaggagtcg gcagcgttga gagacccgac catgggaatc tgcccataga cggcggagtt
     1441 ccccactcct actgttccgt catgacagct caggcacagg ctgctggtga cacccagcgt
     1501 cggctgcgta ttgccctggt tgggattggt cgagctgcta tacggcacat agctcttcgt
     1561 tgacagcttt tgattccaca acggagtgtt ggcgcccaat ccgctgtgcg gagcatggca
     1621 gaacgtgcag cccaggcttc cctgcgtgta ggcggaggcg ccgctggcca ggctcaggtt
     1681 gtgcattccc agaacgtccc cgttgggttg cacctgggcg aagctctgcg ccgcgacaag
     1741 gcaggcgagc gcgatgccgg cgagggtgag gatcggcttc atggctgttt cctcccatcc
     1801 gcggaagcac caatcccgta gtagtggaag atctgcaccc gccggttgaa ggagtcgacc
     1861 acgtagatgc ggtcgtgatt gtcgatgtgc aggccagtcg gcaactgaaa ctgttcggct
     1921 ccggttccct tcccgccgaa gtaatagagc aaccggccct gctcgtcgaa aacctgcacc
     1981 aggccccact cgccgtcaac cacgtacagg tgcccttcgg aatcgaaacc gattccttta
     2041 ggccggaaga tccatcctgc gccgtcgcct accttgccga tcgaatagcg gaacgtaccg
     2101 gagcggtcaa acacctgcac ccggaaattc atggcatcga ccaccgccag atcgttcccg
     2161 ttcaggcgca actcggtcgg gaagttgaac tccccgtcgc cggtgccggt tttgccgatg
     2221 gtctgcagca cgctgccctg catgtccatc acgaagatct tgttgcgcag cgtatcgctg
     2281 acgtacatgc ggcctgcggc cgagtccacg gcaatgcccg tgggacgctt gaaatatcct
     2341 tcgccgccct tcagagctcc caccacgcgc cgaaactttc cgcctgcgtc gaagacgaaa
     2401 atcttgcccg acatcgagtc ggtgacatag atgttgtcct gcgcgtcgac ggcgacgcac
     2461 tgcggcatga gcatgggatt ctttcccacg tcgcgtcgcg aaatgaactt gtatttctgc
     2521 tgatcgaagt cgaagatgtg cacgcccacg gcggaaacgt cggtcacgat gattcggcca
     2581 cgcgagtctg taaccacgcc gtaggggctg gccagatagc ggtaatcggg ctggccggcg
     2641 atgacgtcca ccaggcggtt ccagaaaccg cgcttgggtt tgacctcgcg ctccaggtga
     2701 aaagcgcgct cgaaagagag tttgcgcccg ccgtccatga gcaggtcagg cgcaacaagc
     2761 tggtcggtct ccttggcgtg ctttgccgcg cgcgccggca gcaaggtcac caacagcaac
     2821 ccggccgcca ggccgatgcg cacaggcccg gccgatcgcc ctaaaagaag tcgaaccatc
     2881 tcgatattcc cgcaaaatac gacgtcgtgt tggcggggag ccccgcagcg ctgatgccct
     2941 gcgtgaagcg cgtatacccg gcctgcagac cgacccttcg tagatggtac tgcagctggg
     3001 cgttatagat ttcagtattg ttgtgcgaga aggtctgccc cagggtgttg ctgatggcgc
     3061 gattgtaact gcccgccagc accagccgcc gcatcggagt cactgacaac ccgccgccgt
     3121 agctgtctcc gccgaaggtg atcaggctgc tggctccggg cgtcgcggtc actggaacaa
     3181 atccgcccgc acccagcagc gatactcccg tcgagtgcgt gtagatgcca ttgagggtca
     3241 gctttcgcaa gcccagcgcc gtcgaataac tttcgctgga ggcggtggtc ccggagtaat
     3301 tggtcaggcc gctgtgcatc ccgttgaagg ccgccgtcca ttgtattccc ggcgccacgc
     3361 ggcgatgcac gttcccgctg tactggaagt aagaagtcgt gtaagtgacc agcagcgttt
     3421 gcacattctg cgcgtaactg aagacgcctg ccagctgcca gccctgaatg cggtgaaaga
     3481 aattcacgtt gccgacaaaa cccagggcgt tggtgccctg cccattgctg gtgtcgacta
     3541 cggagccgga gaacgtgaac atgtcgagca gccgcttgcc gtagctcacg gttccgctca
     3601 ggaaggtgcc agtgtagctc ttgcccaaat actcctggtc atagtaagtg gcctgcgtgg
     3661 tgcccgacag gaagcgcgtg aactggtacg tggcgccccc gcccacggtc gacgagtgcg
     3721 cacccgcgcc aaggctgagc ccctgcaccg gcgctcccgt gctggtcagg ctctgcgaca
     3781 ggtagccgtt cagattgctg gtgtaattct cggtgaggtt cagcgaaagc ttctgcgtgg
     3841 gatggaagct ggcgccggca ttcacgatgt cgtcagtgta gctggaggtg ttggagttct
     3901 ctgccgcatc cgagaaataa ttggcatcgg ccgacgcgcg gtcgaagttc acatagaact
     3961 ggccgcggaa tggcagcgcg tgctgcgcgc cgaatccgac gtcatgccca accgagtgct
     4021 ccagcgacga ttcctgccct gaaaggaatt ccgggaactt ggaagagagg ctgttgcgat
     4081 ggaagaaagc gttcagccgg aagccctcga tgtcgtagtt ggagcggaca ttgaacagtt
     4141 tcgtgttgga ggtcgtgtcc tggctggtac cgaagagcgt accgctgccg tcaccctgcg
     4201 agtatcccac ggccagtgtg ggcaagccgg gcaccaacgc gctccagtca atcccgaacc
     4261 cctggctctt gccgatggta gtgaagctgg gctgcccggg caggccgaac gtgcccgtcg
     4321 agttgcggtc gtagcgatag cttaccgagc ccggaaaatg ggtccccgag aacagattcg
     4381 cggtcgcatc gatgccggtc gctccggtca acgactgata gctggaatca ttccgcgact
     4441 ggttgtagta cggcgtggcc gtgaaggaaa gaaaattggg gttgtaatag tagccgttga
     4501 ctctgccgtc gaagccccag gtcagcccgt gactcccagt gatggcgtcg ccgtagtcgc
     4561 cactatagcc gaaggtggcc tggccaccag cgctcagctt ggtgttgtcc ccaatggaca
     4621 gctcaggagt cttttgctgg cttggggttt gcgcgtaaag caggtgtgcc atcaccagca
     4681 ccgccactcc gatgatgcga tttgcgaatt tcattctact gcccttccta ccggagcccc
     4741 tcagttgtcc taataaaggc acaaccgttg ccaaagactg gaaccaagat ggcattccta
     4801 tctcattgaa aataaggcgg ttgccataat ggaactatct ctctacaaaa gtccgaggca
     4861 acgggtgcgg gatatcaccc gtttcgcgag tcgaaagact caatgcccca aagttccttt
     4921 cctctttgga aattccccaa aacgaaaagc ccgccggatg agtcggcggg ctggcaaccg
     4981 gaaggagggg tagatgcgct atcccatctc aatcttcgct ttggcgcgca gttgtttgtc
     5041 gagattggac cgcaacttct cgtacttgct cttctgcaac tcggtcttca gatcgttcct
     5101 gacctcggcc agcggccttt tgtgcgccgg agtgtgcgca ttcatccgaa taatggtgaa
     5161 tgcgctttca atctggatga gccctgtgat ctgtcccggt tgcagggctt gcagaacctt
     5221 gacaacctgc ggcggcagct tgtcggcggc cacaacacgg tggtcgccca tgttcacccg
     5281 gaaatcgtct tcagagatct tttccgccag cagcccgaaa tcctgatagg ttttcgtcgc
     5341 cttggcctgc tgcagtgccg cctcggcttt ctttctcgcg tctttttcct gttccggggt
     5401 cgctttcagc gggggaacga tcgagatgct ctggaagctg aacgattccg gctgctggaa
     5461 tctggcagga ttcttcgcat agtaggcctc gacctcagcc ggcgaaacgg cggacttcaa
     5521 ctccacgtcg gtcttgagaa cctgctcgat cagcagggag cgcttgatct gctgacgcag
     5581 ttgctgctcg gagcctttca tctccgtttg caggtactgc tgatactgat cgggactgtt
     5641 gaattgcttg cggaaatccg cctcggcctg gcttagcttt tcggcgggaa tcgtgagctt
     5701 gcgccgcagc gcttcctgat agaccagctc ttcgaagatg atcatctcca gcgcgccccg
     5761 ccggatctcc gcttcctgcg ccttaggaaa accgttgtgc tgctgggcat aggggaagat
     5821 cgtgtacatc tcgcgcagca aatcgcggtc ggtcagcacc gcgccgttca cccgcgccac
     5881 cggcttgtcc gtcacctgtg gcggcggaac ttgcgccgag gccgggttcg caaccgtggt
     5941 cggcaggtgg gacgcaacct gggtccaggc gaagtgggtg gatgccatca tcagagccag
     6001 cccgaacagc gagcagattg tgagtcggga tctcatattt cctcgcttac cgctatttct
     6061 tcgcttaccc aagaaaaaag gggagaggaa ctcctctccc ctggtgtaat gcagtcaggt
     6121 ctactggaac actgtggtga tgttgttggt gttgttggcc tcgttcgact cgccaaagtg
     6181 gcactggcgg cagaactgcg tcgtcgaagg agcattcgtg ttcgcgacgt tgttgatgtt
     6241 cgggttgtac ggcccgttga tgaagaagta ggtggcatag tacttgccgc cgccgtcgcc
     6301 cgcgaccggg ctgttcttcg acgaagtata aaccgtcatc acgtgctggt tgtggcaggt
     6361 ggtgcagagc acaaagggct tgccgcctgc ggtgacagca taaggattcg accactttcc
     6421 aggcgccagc gcgggatagc cgtagttggc gacgaactgg gcatactggc tgccagcggt
     6481 cggtgggttg aaggctgtac cgctccaggt caggcccggc atcgtggtgg agatgttggc
     6541 attctgtccc accgggtggt cgttggtgta gttgccggct ccggaaccat cattgccgag
     6601 caaagtcggg atggcgccgt tgccataagc cgtgttggtc agcaggccaa tcctttgttc
     6661 ataggactgg ttgaccatca tgttcttcgc ggtgacattg ccgtcatggc agctgaggca
     6721 gagcagaatt ccgcccactt cctggcttcc cgtagcaatg ccgttgggca gaacttccgc
     6781 gtagttgccg ttgtcgccga acgcaatcgt cttcccgtag agtgggctag cgtcctggcc
     6841 ccagagtgcg taggtgccgg tgtcggcagc gccgctgtgg ccggaaccga atgagccgct
     6901 gtgcggagcg tgacatccgg cgcagccgcg gccgccattg ttgtgcgcgc cgaggacgtc
     6961 aaccggcgat gtccagtttt gcgccatcac cagccccccg accgccagaa ccaacatcac
     7021 aatcaggatc gcttttctca taaattctcc cgacctccat gaattgatca cttatgacta
     7081 aactcccagg ggcttaggcc gtgggctgta tggcgcctgg cgccttctgc agccacccct
     7141 cgaccactgt ttatgcaatt ggccgcccga agtggtgcac gcgtgtgagt ttcaaagttg
     7201 aaactgtaag tcattgaatc tgcgtgtgat tcccgcccgc gattcccacg gcaaggactc
     7261 tgaaggtggt gagaactggg ggaaaagcac cagttgcggt tggggtgtgg tcaaattccg
     7321 cagtttatta agacccaaac gtaatgttgc ggatttcaac aagtgggagg g
//
LOCUS       DASPUL010000377         3389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_619249, whole genome shotgun sequence.
ACCESSION   DASPUL010000377 DASPUL010000000
VERSION     DASPUL010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619249"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VEJ00_09165"
     CDS             complement(<1..659)
                     /locus_tag="VEJ00_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01370.1"
                     /translation="MYGYDHKRTEAQFETTWKELQNALPAGGDSGLLLGVSDNKLLLD
                     GIPLETGNAERSFATLLTTAGLASLHFSKETTEEDFVRMVRAFTVAGSKAQDVAKQIK
                     EALGSGKQNSTIKINEVKFVAADPMTGDVSIAAQIAAQTLGPEFKQWLNDPQKLLQLI
                     AAAEGANAGGGGGGQGTGPGTGVPMVPLGSVPNVPRGSGKGVWVPEGTPIGTPGGTAA
                     GA"
     gene            991..2541
                     /locus_tag="VEJ00_09170"
     CDS             991..2541
                     /locus_tag="VEJ00_09170"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HXX01371.1"
                     /translation="MLRVLDECHHVSADFSADPVHDLRVALRRCRSMADGLMAMDPDP
                     GWKAMKKAGKQLFRRLGELRDIQIMMDWIEKLDVARALPPALPEAIDGGEGRNDYPVN
                     EPRDPAAHALLGILRHRESQQKHEARAALEEFDAKQWKHWSKSLPQRAAHIRPGSAVF
                     KHLALERWTAARELHARAMRNRSQVALHTLRIGIKRFRYIVENFLPAEHKTWSNDLKH
                     MQDLLGEVHDLDVLWATALASRVFPDDDSRKRWHARIIEERTKRVDEYRKRMVGPDSL
                     WNAWRAGLPQGKQIQAIATRRMKLWAKALDPDFAHSEHVARLALELHDGLSSRGLLAP
                     TNGSEGDLRSTLYLAALLHDVGKARGDKGHHKASLDLIRAHGTPLGWGAEIMQRAAVV
                     ARFHCGALPTRSHKTLRDLLSDEQRIIIRLSAILRLANAFDTAHDGHIRRVQIESPNA
                     EKRRTNGFWRKPPVLGKNEAVVISAVGYLPGSPTAQAIAAERYLLETVLRRPVIVKAM
                     VLARQRAA"
     gene            2641..3192
                     /locus_tag="VEJ00_09175"
     CDS             2641..3192
                     /locus_tag="VEJ00_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01372.1"
                     /translation="MTHAQLVEKAVRWLRSYRCGVVLSEQACVSGEMPDAIGWKRASH
                     SVLVECKVTRADFLADRDKPFRQKPEQGVGCERFYLTPLALVKAEELPGGWGLLEYRR
                     GRIEMLQPSATNLRTSKGFGYEMNLLLASLRRVEVRIEPQSITDFLKWKNRMAEYNRG
                     ALPQGLTSSEAEMNIFLEQEPTG"
BASE COUNT          655 a         1081 c         1002 g          651 t
ORIGIN      
        1 gcacccgcgg ctgtgccgcc cggagttccg attggcgtac cctcaggaac ccacactccc
       61 ttgcccgatc cccgcggaac attcggcacg ctgcccagcg gaaccatcgg aacgcccgtg
      121 ccggggccgg tcccttgtcc ccctccaccg cctccggcgt ttgccccctc ggcagcggca
      181 atcagctgca acagtttctg cgggtcgttc aaccactgct tgaattccgg tcccagggtc
      241 tgcgcggcaa tctgcgcggc gatgctgaca tcgccggtca tcggatcggc ggccacaaac
      301 ttgacttcgt taatcttgat cgtgctgttc tgcttgcccg agcctagagc ttccttgatc
      361 tgtttggcta cgtcttgcgc tttcgaacct gcgacggtaa aggctcgcac catgcggaca
      421 aagtcttcct ccgtagtctc tttcgagaag tgcaggctgg ccagtccggc cgtggtcaga
      481 agggtcgcaa aactccgttc cgcattgccg gtttcgagcg gtatgccgtc gagcaggagt
      541 ttgttgtcgg agaccccgag cagaagtccg ctgtctccgc ccgccggcag cgcattttgc
      601 agctctttcc aggtggtctc gaactgcgcc tccgtgcgct tgtggtcgta gccgtacatc
      661 cgcgcatact tgatcaggat gttcagacta tggacaaatg cgcgcgccga agccgagcgg
      721 gcgccttgtg atgtgttcgt gccggagata agacactcct cgtaacgcag ctttgaacct
      781 taaggttaaa gggaaaaggt aaggcgttaa acgttacgga ggtaattggt cggttctaac
      841 cgttctctaa ccgttccgca cggtcagagg atcgggcaga gtagcaccgc taagggaatg
      901 ggcatccagt agaatacaga cggagccttt taggcccccg cagccgagaa cttgaatcct
      961 gccaacaaaa ctactggttt gcgctgttgg atgctgcgcg tactcgacga gtgccaccac
     1021 gtgtcggcgg atttcagtgc cgatccggtg cacgacctgc gtgtggcgct gcgccgttgc
     1081 cgctccatgg ccgatggcct gatggccatg gatcccgacc ccggctggaa agccatgaag
     1141 aaagccggca agcagctgtt ccgccggctg ggcgaactgc gcgacatcca gatcatgatg
     1201 gactggattg agaaactgga tgtggcgcgg gcgctcccgc ccgcgctgcc cgaggcaata
     1261 gacggcggtg agggacgcaa cgactatccg gtcaacgaac cccgcgatcc cgcggcacac
     1321 gccctgctcg gcatcctgcg ccaccgcgag tctcagcaga agcacgaggc ccgcgccgcg
     1381 ctcgaagaat tcgacgcaaa acagtggaaa cactggagca agtctctgcc gcagcgagcg
     1441 gcgcacattc gccccggaag cgccgtcttc aagcatctcg cgctcgaacg ctggaccgcc
     1501 gcccgcgaac tccacgcccg tgccatgcgg aaccggtccc aggttgcctt gcacacgctg
     1561 cgcatcggca tcaagcgctt ccgttacatc gtcgagaact tcttgcccgc cgagcacaag
     1621 acctggagca acgatctcaa gcacatgcag gatcttctgg gcgaagtcca cgatctggat
     1681 gtcctttggg cgaccgccct ggccagccgc gtctttcccg acgacgactc ccgcaagcgc
     1741 tggcacgcgc gcattatcga ggaacggacc aaacgagtcg acgagtatcg aaaaaggatg
     1801 gtcggcccgg attcgctgtg gaatgcgtgg cgcgccggtt tgccccaagg caagcagatt
     1861 caggccattg ccacccgccg catgaagctc tgggctaagg cgctggatcc cgatttcgcc
     1921 cattctgaac atgtagcccg cctggcgctg gagttgcacg atggcttgag ttcccgcgga
     1981 ttgctggccc cgacgaatgg atcagagggc gacctgcgtt ccacgctcta cctggccgcg
     2041 ctcctgcatg acgtgggcaa ggcaagagga gacaagggcc atcacaaggc atcgctggat
     2101 ctgatcaggg cgcacggcac gccactgggc tggggcgcgg aaatcatgca gcgcgccgcg
     2161 gttgtcgccc gcttccattg cggtgccctg cccacgcgaa gccacaagac gctgcgcgac
     2221 ctgttgtcgg atgagcaaag gatcattatt cgtctctcgg ctattcttcg cctggccaat
     2281 gccttcgata ccgcccacga cggccacatc cgccgcgtgc agatcgagag cccaaatgcc
     2341 gaaaagcgcc gaacaaacgg cttttggcgt aagcccccgg tgctcgggaa gaatgaagcc
     2401 gttgtcatct ctgccgtagg ctacctgccc ggcagtccca cggcacaggc catcgctgcc
     2461 gagcgctatc tgctggaaac ggtattgcgc cggccggtca tcgtaaaagc gatggtgctt
     2521 gcccggcagc gcgcagcgtg attccttcgt gttcctttgc gtcctttgtg gtcaacgctc
     2581 tcctggaaac ttcccagtca cttatcgcgg cgggtcattc cgcgctacaa cagaccttct
     2641 atgactcacg cgcaactggt cgaaaaagcg gtgcgctggc tgcgttccta ccgctgcgga
     2701 gtcgtgctct ccgagcaagc ctgcgtcagc ggagaaatgc ccgacgccat cggctggaag
     2761 cgcgccagcc attccgtgct ggtcgagtgc aaagtcacgc gcgccgactt cctggccgac
     2821 cgcgataagc ccttccgcca gaaacccgaa cagggagttg gctgcgaacg tttctatctg
     2881 acgccgctgg ctttggtgaa ggcggaagaa cttcccgggg gctggggatt gctcgaatac
     2941 cgtcgcggcc gcatcgagat gctacagccc tcggcgacga atctgcgcac atccaaggga
     3001 ttcggctacg agatgaacct gttgctcgcc agcttgcgcc gggtcgaggt acgcatcgag
     3061 ccgcagagca tcaccgactt tctcaagtgg aaaaaccgta tggccgaata caatcgcggt
     3121 gcgctgccgc agggactgac gtcgtccgag gccgagatga acatcttcct cgaacaggaa
     3181 cccactggct gatctccacc gattgctgta gcccttgaga tacccagcgg tccgcaaaaa
     3241 aagaaaaccg ggctggttgg cccggcttct tgtgcgccgg gcggatgcgg ttacatcaac
     3301 acccggcgcg actgaccgct gttttcgctg gccagtcgta aatcgctggc aaatctcgaa
     3361 gccctgtctc gcacgcattc tgccaattt
//
LOCUS       DASPUL010000378         4229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1569325, whole genome shotgun sequence.
ACCESSION   DASPUL010000378 DASPUL010000000
VERSION     DASPUL010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569325"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..679)
                     /locus_tag="VEJ00_09180"
     CDS             complement(212..679)
                     /locus_tag="VEJ00_09180"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HXX01373.1"
                     /translation="MRMKKIVCCGFVVVLAAMLATVPAVAGDKSDVAARAATWEKEYN
                     AGNLAAVAALYATDGCRMPPNQEAVHGTEAILAQLKAGKDRGMAKVKIVVTSAESSGD
                     VGYGTGTYEVMGADGSHIDHGKWMLVSKKVNGTWKTQCDTFNSDMPLPTGGAK"
     gene            complement(918..1736)
                     /locus_tag="VEJ00_09185"
     CDS             complement(918..1736)
                     /locus_tag="VEJ00_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01374.1"
                     /translation="MKTTQAWAWLTAAVMAAGLNASYHDGGLQWAHRIAEQAGHSSRA
                     VLALARGHADQFLEEARLATRGESAPCAVRVVLTRVQEKVAGEQQRVAWVQEKIADGE
                     QGVAQIEEMSARQREHLARLEEMRARIEERLAERAARFRGAQAMRMTAWEMPPACPRQ
                     RVNAPAVEVPGVEVPEIEVQGIQVPAISVQGMQLQPVDVQGIDVPAVDVPAIQIPAIH
                     VPAVHVPAIHVRAAQIPRIQIPQIQIPQIQVPAIKVPEVRVPAFRVETSDAGMV"
     gene            complement(1849..3288)
                     /locus_tag="VEJ00_09190"
     CDS             complement(1849..3288)
                     /locus_tag="VEJ00_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01375.1"
                     /translation="MYSSNVSNSLRRSSRVPTVLPVLVTTLDGSPFSEVCETLVVNAH
                     GCLMVSPAKLDTGVPVRFHTKEGREATAHVVSCQPIGEESQAWRLGARLDRPENFWGL
                     KDCPRDWALPSVVVQTRPPQSLPSTVLPAHTMPAPVAPTDAMIERASRQLEERVKRMI
                     KEFVQPLEAEVATLREQLAERQARPPRFEVSLSSIPPELEQQLETRLKKNLEPRALEE
                     ARQQYTNLLAAARATIDQKTREGYEDFVQRVGRELEVVEKRGHDISEHLSETTRQHLR
                     RGLEDFRQQLLEGGNSLKRLSDELLQFVQDSVSAEHSARLAELEQLRSAVTSESARLH
                     EHIEYLDVRIRNLDESVRNMEAGLDKRLGKMVSNTVKDARSQMESAGSDILEEWTARG
                     AAAAANQVDEASAKMKMVQEEIVASGHESLRIQVTSALQEFERSMEEVARRSVEHWRL
                     KLAGGLNVVLRSLGDQFQLQAGSDEEDPR"
     gene            complement(3660..>4229)
                     /locus_tag="VEJ00_09195"
     CDS             complement(3660..>4229)
                     /locus_tag="VEJ00_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01376.1"
                     /translation="DQPTQFGNTVAITPLDANNWKMVIKKDGRVLSSMTHTLSADGKT
                     QTIKGTGNKPDGTTDDFTVVMKKVSGGSGWGGTWEDTDVKFTSPDEWEIQAYEGDGLS
                     FNTAAYKDTLSMKFDGKDYEEKGPNVAPGSMSSGKRVDAHTLELTDKVKGEVMDHTKF
                     EVSPDGKTLTLTVHGTGQANAQTYVYNKM"
BASE COUNT          779 a         1391 c         1164 g          895 t
ORIGIN      
        1 gcgagcctcc tgacgaaggc aacactcgcg caagcttctc aaatgtagca atcaagcgtg
       61 agcactcaaa acccctgccg gggaattttg agtgaaatgg cgatgttatg tcaaacatcc
      121 gttatccatg cttcaattga cccactagac agacgacaaa acggcgcacc cgcgagcctt
      181 cttgggacat ccgagtgcgc caacccagac cttatttcgc acccccggtc ggcaggggca
      241 tgtccgaatt aaaggtgtcg cactgggtct tccacgtgcc atttaccttc ttcgacacca
      301 gcatccactt gccatggtca atgtgggaac cgtctgcacc catgacctcg tacgtccccg
      361 tgccgtagcc cacatcgccg ctgctctcgg cggaagtgac gacgatctta accttcgcca
      421 ttccccgatc cttaccggcc ttgagctggg ccagaatcgc ctccgtgccg tgaactgctt
      481 cttgattggg tggcattcgg cagccatcgg tagcgtacag cgccgcaaca gccgcaaggt
      541 tgccagcgtt atactctttc tcccaggttg cggcccgagc cgccacatcc gacttgtcac
      601 cagccaccgc cggaaccgtc gcgagcattg ccgctaacac gacgacaaat ccgcaacaca
      661 caattttttt catccgcatg tctcctcctt ttcgaaaaac ggaacgcgga taaatcagat
      721 tacgcaggtg attgtagtcc aaccgcctga ccagtgcggc tcgcagacct gtagttgatg
      781 aaatcaggcg attgcactca ttgacccacg gacccagagg cgacacggaa cctgtcgcaa
      841 gaacccgtca gacagaaaca acgagaaggg gcgcggctgg tttgccgcgc ccccgaggag
      901 gaaacgaagc ttcgctgcta caccattccg gcgtctgacg tctcaacccg aaacgccggc
      961 acccggactt ccggaacctt aatcgctgga acctggattt gtggaatctg gatttgcgga
     1021 atctgaatcc gcgggatctg ggcggcgcgc acatgaattg ccggaacatg gactgctgga
     1081 acatggatcg ccggaatctg gatggccgga acatcgactg ccggaacgtc gataccctgg
     1141 acgtctaccg gctgaagctg cattccctgc actgagatcg ccggaacctg aatgccctga
     1201 acctcaattt ccggaacctc aactccggga acttcgaccg ctggcgcatt cactcgctgc
     1261 cgcggacaag ccggaggcat ttcccaggcc gtcatccgca tcgcctgcgc gccccggaac
     1321 cgcgctgccc gctctgccag ccgctcttcg atccgcgccc gcatctcttc gagccttgcc
     1381 aggtgctctc gctgccgggc cgacatctct tcgatttgcg cgactccttg ttcgccatcg
     1441 gcaatctttt cctgcaccca ggctactcgc tgttgttccc ctgcaacttt ctcctgaacc
     1501 cgcgtcagca ccacccgcac cgcgcacggc gccgactcac ctcgcgttgc cagccgggct
     1561 tcctcgagga actggtcggc gtgtcccctc gccagcgcta acaccgcccg cgagctgtgc
     1621 cccgcttgct cggcgatccg atgcgcccac tgcaatccgc catcgtgata gctggcgttc
     1681 aaccccgccg ccatcaccgc cgccgtcaac catgcccagg cttgtgtcgt tttcatcgcc
     1741 ttaaccctac ctgccagata gacgggagcc ctgaagatcg gttagcaggg gtcaagaaag
     1801 gaacaagggc ggagattttc acaacaggtt tacagtccag cgcacgactc agcgcgggtc
     1861 ttcttcgtcc gagccggctt gcagctggaa ttgatcgccg agactcctca agacgacatt
     1921 caggccgccg gccagcttga gccgccagtg ctcgacagaa cgccgggcca cttcttccat
     1981 ggagcgctcg aactcctgca aagcactggt cacctgaatc cgcagcgact cgtggcccga
     2041 ggcgacgatt tcttcctgga ccatcttcat cttcgcgctg gcctcgtcga cctgattcgc
     2101 cgccgcggcc gcgccgcgcg ccgtccactc ctcgagaatg tcgctgcccg cgctctccat
     2161 ctggctgcgc gcatccttga ccgtgttgct gaccatcttg cccagacgct tgtccaggcc
     2221 agcctccatg ttgcggacgg attcatccag gttccggatg cggacgtcca gatactcgat
     2281 gtgctcatgc agccgcgccg actccgacgt caccgcagat cgcagctgct ccagctccgc
     2341 cagccgcgcg ctgtgctcgg cgctcacgct gtcctgcacg aactgcagaa gctcgtcgct
     2401 caagcgcttg agcgagttgc cgccttcgag caactgctgg cgaaagtcct ccagcccgcg
     2461 ccgcagatgc tggcgtgtag tctccgacag gtgctcggaa atatcgtgtc cgcgcttctc
     2521 tacgacttcc agttcacgcc cgacgcgctg cacgaaatcc tcatagccct ctctggtttt
     2581 ctggtcaatg gtcgctctgg ccgcggccag caggttcgtg tattgctggc gagcttcctc
     2641 gagcgctctg ggctcgagat ttttcttcaa gcgcgtttcc agttgctgct ccagctcggg
     2701 aggaatgctc gacagcgaca cttcaaagcg cggaggccgc gcctgccgct ccgccaactg
     2761 ctcgcggagc gtggcgacct cagcctcgag cggctgcaca aattccttga tcatccgctt
     2821 cacacgctct tccagctgcc gcgaagcgcg ctcgatcatg gcgtcggtgg gcgccaccgg
     2881 cgcaggcatc gtgtgggcgg gcagaaccgt cgacggaaga ctttgcggcg gccgggtctg
     2941 caccaccacc gagggcagcg cccagtcgcg cggacaatct ttcaatcccc agaaattttc
     3001 cggccggtcg agccgcgctc ccagccgcca ggcttgactc tcctccccga tcggctggca
     3061 ggagacgacg tgggccgtcg cttcgcgtcc ttccttggtg tgaaaccgca cgggcacgcc
     3121 ggtgtcgagc ttggccggcg acaccatgag gcatccgtgg gcattgacca ccagggtttc
     3181 gcacacttcg gagaacggcg acccgtctaa agtagtgacc aggacgggca atacggtcgg
     3241 cactcgcgaa ctgcgccgca gtgagttgga gacgttgctg ctatacatgc cactctgcct
     3301 catgttgcga gggatcggtg ccgggggcct gtggggccga gcgccacgta ctgtctaaga
     3361 tagccctaat gcggccgcga gccgacaggc cggaagtgta gtaacgaggg tgtccgttgg
     3421 tacaggaggc ggggcggagt tgggacgagg cgggattgag cgcgataagt gtaatccgga
     3481 tgtttcgcca actgactcgc cgcagcgatt gtgcagtctg ctcattgcct tggtccgagc
     3541 gcggtttcta tgatctgtac ggctttacca gtcccatcaa tgatttttct tgaggacagt
     3601 cctctcccta ggcaagcggg ttcgcacacc aacgccataa gcaggactgt gccgcggagc
     3661 tacatcttgt tgtagacgta agtctgggcg ttcgcctggc cggttccgtg aaccgtgagc
     3721 gtcaacgtct tgccatcagg cgagacttca aatttcgtgt ggtccattac ttcgcccttc
     3781 accttgtcgg tgagttcgag cgtgtgtgcg tctacacgct tgcctgacga cattgagccg
     3841 ggcgcgacat tcggaccctt ttcttcgtaa tccttgccgt cgaatttcat gctgagggta
     3901 tctttgtaag cggccgtatt gaaactcagg ccgtcgcctt cgtaggcctg aatctcccac
     3961 tcatcgggag aggtgaattt cacgtcggta tcctcccaag tcccgcccca acccgagccg
     4021 ccgcttactt ttttcatcac aaccgtaaaa tcgtcggttg tgccgtcggg cttgttgcct
     4081 gttcccttaa tcgtctgcgt cttcccgtct gccgaaagcg tgtgagtcat ggaactgaga
     4141 accctgccat ccttcttgat caccatcttc cagttgttgg catccagggg agtaatcgct
     4201 accgtgtttc cgaattgggt cggctgatc
//
LOCUS       DASPUL010000379         5222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1988489, whole genome shotgun sequence.
ACCESSION   DASPUL010000379 DASPUL010000000
VERSION     DASPUL010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988489"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1354
                     /locus_tag="VEJ00_09200"
     CDS             215..1354
                     /locus_tag="VEJ00_09200"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HXX01377.1"
                     /translation="MSALAAKSRLAYIDWMRGLACVLMFQTHCYDSWLGGAARQSRFF
                     MFSQLGGTFPAPLFLFLAGISFALVTEKLYQKNLPPAQIARSTIRRGAEIFAFGLLFR
                     LQEYAIAWGWAPKSDLLRVDILNTIGLSMMLMGLVCWMVSAVQRGPCSRRALVFSAAG
                     TALVISLLTPLLWTIWRPRFLPWPLESYINGVHNLGTPQPWLFPVFPWAAFAFAGLGA
                     GFMLQSSWSRTHETQVFLSLGAAGVALIELSRWLDGQPRQIYPVYDYWHTSPNFFLVR
                     VGMLLVILTVSYAWCRWGAAQWGFSPLIQLGEASLVVYWVHIEFVYGRFSILPKHAQG
                     IRTASLGLLVIFLAMLALAFARTHTKGWMKEQRIPWLRRRPVATI"
     gene            complement(1377..2324)
                     /locus_tag="VEJ00_09205"
     CDS             complement(1377..2324)
                     /locus_tag="VEJ00_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXX01378.1"
                     /translation="MQTIGAFLLTRRIHLPSAEERLVEVAPGIKVRCWCHWVSDRKDD
                     RAKTLTLIVVHGLEGSTDSQYMLGVARNGLAAGINVVLMNQRNCGGMDYFAPTLYNSS
                     LSGDVAAVARHVIENDSVSRFALVGFSMGGNLVLKLAGEWGSQGPPQFRGVAAVCPAM
                     DLAASADALHEPANRIYEMYFLMQLSRRMRRKAKFFPARFDVARLRGVSSLRLFDDRV
                     TAHYCGFAGADDYYTRAAAANVVERISVPALIIHAANDPFIRMLPETRRRILKNPNIT
                     YVETADGGHCAFVGVKNGDPGDDGRWAEREVVDFARQFA"
     gene            2902..3681
                     /locus_tag="VEJ00_09210"
     CDS             2902..3681
                     /locus_tag="VEJ00_09210"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXX01379.1"
                     /translation="MLQLNPKTTVATPQAPATEKRQPRRLQIALALLVMALAVTIARD
                     RDFWFGTGAEDSDVLGPEVTHQSAKSAVPSAAPSVPTSGAKKQAVAQKPAGTNAPESS
                     IVASNRTVVPPLDVEVVAGDTHHTIHPGNNATKVEIGNAASALQSSKPLNPATNAAER
                     EQISTALQPQQSLEANYPLLAQQMKVQGSVVLQALIGADGVIQDIHVLSGPSILASAA
                     QQAVRQWRFKPYLENGQAVETQAKITVNFTIKVADNSPRNS"
     gene            3893..4222
                     /locus_tag="VEJ00_09215"
     CDS             3893..4222
                     /locus_tag="VEJ00_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01380.1"
                     /translation="MDRRRERRVFVELPVKIWGMDANARPFTQPASLRTISGRGATLQ
                     GVSAQLKPGTVVDLQYEGVKAQFRVVWLGRPGTEMQGEVGVENLSADVQLWDVDPLRC
                     AASVGQG"
     gene            4256..5179
                     /gene="pqqB"
                     /locus_tag="VEJ00_09220"
     CDS             4256..5179
                     /gene="pqqB"
                     /locus_tag="VEJ00_09220"
                     /inference="COORDINATES: protein motif:HMM:NF003523.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HXX01381.1"
                     /translation="MKILGSAAGGGFPQWNCACANCRALRAGTFQGKARTQAQVAVSE
                     DGLLWFLLGASPDLLAQIEATPELHPRRGMRHSPIAGVALANADLDHVLGLLLLRELQ
                     PLRIHATASVRRILREDNSMFGMLHRIPDQAAWTDFQSGKEFSLCDAQGQDSGLICRA
                     LSLSTHYPSYVTAERQSQLLPGEASLGFILHSTSGARLAYMPAVPRVDDTSLAELNAC
                     DALLFDGTFWSNTELMAVNDDPRTGPTAQQMGHVPVVETLVELAGLHRPRKIYIHINN
                     TNPMLNEAGPEYRAVREAGWEIAEDGWQFDL"
BASE COUNT          999 a         1676 c         1459 g         1088 t
ORIGIN      
        1 ggctggaaag aaggagcgca gcgaacaatg tcgaaatgag caacttcata agttctttag
       61 gaactccatg cacgtgcctc atattagcct tgcttcagct tacaccggct tacccatact
      121 tcgattgatc ccacagtaga gatggttgca ccctcggctg aagcggcgag gatcaacggt
      181 ccacaacgta tacttcccct ggctattctc gctcatgtcc gcacttgcag ctaaatcacg
      241 cctcgcctac atcgactgga tgcgcggact tgcctgcgta ctgatgtttc agacccactg
      301 ctacgactcc tggctcggcg gcgcggcacg gcagagccgg ttttttatgt tttcgcagct
      361 gggcggaacg tttcctgctc cccttttcct ttttctggca gggatttcgt tcgcactggt
      421 taccgaaaaa ctctatcaga agaatctccc gcccgcgcag atcgcccgca gcactatccg
      481 ccggggcgct gagatcttcg cctttggact gctcttccgg ctgcaggaat acgcgattgc
      541 ctggggctgg gcgccgaaaa gcgacctgct ccgggtcgac atcctcaaca cgatcgggct
      601 ttccatgatg ctgatgggtc ttgtctgctg gatggtctct gccgtgcagc gcgggccctg
      661 ctcgcggcgg gctcttgtgt tctccgccgc cggaaccgcg cttgtgatct ctttgctgac
      721 accgctgctg tggacgatct ggcgacctcg ttttcttccc tggccgctcg agtcctacat
      781 caacggcgtg cacaacctcg gcacgccgca gccctggctg ttccctgtct ttccttgggc
      841 ggcctttgcg ttcgctggct tgggggccgg attcatgctg caatcttcct ggtcgcggac
      901 tcacgaaacg caagtcttcc tgagtcttgg ggcagccggc gtggcgttga ttgaactctc
      961 gcgctggctg gatggccaac cccgccagat ctatcccgtg tacgactact ggcacaccag
     1021 tcccaacttc ttccttgtcc gagtgggaat gttgctggtg attctcacgg tgagctatgc
     1081 atggtgccgc tggggtgcag cgcaatgggg attcagccct ctcatccagc tcggcgaggc
     1141 gtcattggtc gtttactggg tgcacatcga attcgtgtac ggccgcttct cgatcctgcc
     1201 caagcacgca caaggaattc ggaccgcttc gttgggcctt ttggtgattt ttttggcgat
     1261 gctagccctc gcattcgctc gcacgcacac gaagggatgg atgaaagaac agcgtattcc
     1321 gtggctgcgt cgccgcccgg ttgcgacaat ttaagttcag ggaagatcct ctcgcatcaa
     1381 gcgaattgcc gcgcgaagtc taccacctca cgctcggccc atcgcccgtc atcccccgga
     1441 tctccgttct tcactcctac aaacgcgcag tggccaccat cggcggtttc aacgtaggtg
     1501 atattcggat tcttcaagat tcggcgccgc gtctctggca gcatccggat gaacggatcg
     1561 ttggccgcat gaatgatcaa tgcaggaacg gaaatccgct ctacgacatt cgcagctgcg
     1621 gctcgggtgt agtagtcatc cgcaccggcg aatccgcagt agtgcgcggt gacacgatcg
     1681 tcaaacagcc gcaggctcga aactccgcgc agtcgcgcca catcgaaccg cgccggaaag
     1741 aactttgcct tgcggcgcat ccgccgcgac agttgcatga gaaagtacat ctcgtagatt
     1801 cggttcgccg gctcatgcag cgcgtccgcc gatgccgcca ggtccattgc cggacacact
     1861 gccgccaccc cccgaaattg tggcggtccc tgactgcccc actccccagc cagcttgagc
     1921 acaagatttc cgcccatcga gaagccgacg agcgcgaagc gcgaaacact atcgttctcg
     1981 atcacatgcc gcgccacagc ggcgacgtcc cccgaaaggc tcgagttata gagcgtaggc
     2041 gcgaaatagt ccatcccgcc gcaattccgc tggttcatga gcactacgtt gatgcccgcc
     2101 gccagtccat ttcgcgccac ccccaacatg tactgggaat cggttgaccc ttccagcccg
     2161 tgaaccacga tcaaagtgag cgtcttcgcc cgatcgtcct tccggtcaga gacccaatga
     2221 caccagcacc gcaccttgat gccgggcgca acctcaacca acctctcttc cgctgagggg
     2281 agatgaatac gccgcgtcag caaaaaggcg ccgatcgtct gcatgtgccc gccccgtagc
     2341 cagggatgcg gttggaacgg agcacgactg ctccactgca tccgtctgcc atcgtctgac
     2401 cgggaggtca gccgggcgcc ctggccaatt tgcgaagtcc ccacaaattg attctaatgg
     2461 agatgtatct ggagctgccc atgcccattt ttgagtttgt ctgcggggaa tgccaacacc
     2521 ggttcgaggc tctcgtctac ggccaacaga agcccgaatg ccccaaatgt cacgccacga
     2581 agctggagcg tcagctctcg gtgtttgccg ttggggctaa gggggcatcg gcctcgccga
     2641 ccggtgcctg tggcgcctgc ggtgaccctc ggggtcccgg cgcctgctcc ttgggcgata
     2701 tggattagcg ctccccgggg aatcgcgcca ggagtggctt ttgcaactct aaccacatga
     2761 aaaagaacgt ctttctagcc aggggaacat ggcccagtcg tgcctctcat cccccctccg
     2821 gtgccattgc cgcgctcgca aaaatgctgc aacctattgg cgtcgcgtaa cttcccaggt
     2881 ttcgcgaagc ccaggtcgaa catgctgcag ctgaatccga aaacgacagt cgccacccct
     2941 caagctcctg ccaccgagaa gcggcaaccc cgccggttgc agattgcact cgccctgttg
     3001 gtgatggcgt tggcggtcac tatcgcgaga gaccgcgact tctggttcgg caccggagct
     3061 gaagattctg acgttctcgg ccccgaggtc acgcatcaat cagcgaagtc ggctgttcct
     3121 tcggctgcac cctcagtacc aacttccggc gccaagaagc aggctgtcgc gcagaaaccc
     3181 gccgggacca acgctccgga atcgagcatc gtagccagca atcgcactgt ggtccctcct
     3241 ctggatgtcg aggtcgtagc cggcgatacg caccatacga ttcaccccgg caacaatgca
     3301 accaaagtcg agatcggcaa tgccgcttcg gcactgcaga gttcaaagcc gctgaatccg
     3361 gccaccaacg ccgctgagcg tgaacagatc tccaccgccc tgcaaccgca gcaatcgctg
     3421 gaagccaact acccgctgct ggcgcagcag atgaaagtgc agggatcggt cgtcctgcag
     3481 gcgttgattg gtgccgacgg ggtcatccag gacatccatg tgctgagcgg accgtcgatt
     3541 ctagcctcag ctgcgcagca ggccgttcgc cagtggcgct ttaagcccta cctggagaat
     3601 ggtcaggcag tcgagaccca ggccaagatc accgtcaact tcaccatcaa ggtcgccgat
     3661 aattctccgc ggaacagcta gaacttctct cgactctcag gcggacagtc tcttgcccga
     3721 tcagtaatcg taagcgcttg aagcgatacg ctttggccat ctcttttcac cacatgccaa
     3781 atctgcggaa ggtccggtaa cttccggaac cttcgcgcgg taacgcgggt tacctgtgtt
     3841 tctctctttt gctctcttac gatcatcggg atcgccgtta gcgaggctgc caatggaccg
     3901 tcgccgcgaa cgccgtgttt tcgtcgaact gcctgtaaag atctggggca tggacgccaa
     3961 cgcccgcccc tttacccaac ctgccagcct ccgcaccatc agtggccggg gagcgacgtt
     4021 acagggcgtg agcgcacaac tgaagccggg caccgtcgtc gatctgcaat atgagggagt
     4081 gaaagcccag ttccgagtcg tctggctcgg gcgaccggga accgagatgc agggagaagt
     4141 aggggtggaa aacctctcgg ccgacgtgca actctgggac gtggaccccc tccgctgtgc
     4201 tgccagcgtc ggccagggct gacctcgcct ttcttgctac actccaagcg tgcgcgtgaa
     4261 gatcctcggc tcggccgctg gcggtggatt ccctcagtgg aactgtgcct gcgcgaactg
     4321 ccgcgccctg cgtgccggaa cattccaggg gaaggcgcgc acccaggcgc aagttgccgt
     4381 cagcgaggat ggcctcttgt ggttcttgct gggcgcatcc ccggacctgc tcgctcagat
     4441 cgaggctacg ccggaattgc atccgcgacg aggtatgcgc cattcgccca tcgccggagt
     4501 cgcgctcgcg aatgccgacc tcgaccatgt cctcggactc ctgctgcttc gggaacttca
     4561 gccactgcgc atccatgcca ctgcctccgt tcgccgtatt ctccgcgaag ataactcgat
     4621 gttcggcatg ctgcatcgca ttccagatca ggccgcgtgg actgattttc aatctggaaa
     4681 agaattctct ttgtgcgacg ctcaaggcca ggattccggt ctcatctgcc gcgcgttgtc
     4741 gttgagtact cactatccct cctatgtgac ggcggaacgg caatcccaac tcttgcccgg
     4801 cgaggcttcc ctgggattca tcctccattc gacttccggc gcacgcttgg cctacatgcc
     4861 cgccgtgcct cgagtggacg acacatcgct cgcggaactc aatgcctgcg acgccctgct
     4921 tttcgacggc acgttctgga gcaacaccga actcatggcc gtgaatgatg acccgcgaac
     4981 cgggccaacc gcgcagcaaa tgggccacgt gccggtcgtg gaaacgctcg ttgagttggc
     5041 gggactgcac cgtccccgta aaatctatat acacatcaac aacaccaacc ccatgctgaa
     5101 tgaggctggg ccggagtatc gcgcagtgcg cgaggcaggc tgggaaatcg cggaggatgg
     5161 atggcagttc gatctgtagc caatacgacg gacctgctgt cgttcgacga gttgcgcgct
     5221 ca
//
LOCUS       DASPUL010000380         5322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_620632, whole genome shotgun sequence.
ACCESSION   DASPUL010000380 DASPUL010000000
VERSION     DASPUL010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620632"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1036
                     /locus_tag="VEJ00_09225"
     CDS             278..1036
                     /locus_tag="VEJ00_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HXX01382.1"
                     /translation="MQSRINGTTMPVLEFILEPNESIISEAGELSWMSSSIQMTTHTQ
                     FAGGGGIFGVLKRVAGGGSLFMTEYRAVGAAGELAFATKLPGHIVPVEVAPGHEYMIH
                     RHGFLCATSQIQIGVGFQQSLGAGIFGGDGFLLQRVSGQGTAWLELSGELVVRDLQAG
                     ETLRVHPGHVGAFQSSVSFQITTVPGIKNMIFGGDGIFLAALTGPGRIWLQTLPIAKL
                     AHAIDHYLPHEVRRQTVEGGVVGGIVGSILDGMK"
     gene            1045..2124
                     /locus_tag="VEJ00_09230"
     CDS             1045..2124
                     /locus_tag="VEJ00_09230"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXX01383.1"
                     /translation="MNRRPCLFAALMFLAPFVVAQEAPRYPITEGDFVAHDFKFQSGE
                     QLAELRLHYRTLGTPARDADGNVTNAVLLLHGTGGSGAQFLAPQFADELYGPGQPLDT
                     NRYFIIAADGIGHGKSSKPSDGMHAHFPQYDYDDMVAAQHLLLTDGLKVQHLRLIFGT
                     SMGCMHSFVWGEAYPDFMDALMPMACLPVQIAGRNRLWRKMLMDAIREDPDWKDGEYK
                     EEPARALRTAQDLLILAGSAPVLMQKTLPTRGAADKYLADAEGRPGTPPDANDLLYQV
                     NASRNYDPSPQLEKITARVMWINSADDFINPPELGIAEKECKRLKNGTFVLLPISEKT
                     HGHGTHTWAVAWKEYLVQTLALAGH"
     gene            2235..3041
                     /locus_tag="VEJ00_09235"
     CDS             2235..3041
                     /locus_tag="VEJ00_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXX01384.1"
                     /translation="MINGKRIAVVMPAYNAEKTLEATVGEVPELTDIRILVDDHSSDQ
                     TVELARRLGLLVFQHDRNYGYGRNQQTCYREALEAGADVVIMLHPDYQYTPLLITAMA
                     SMVAYDVYDVVLGSRIIGGRALRGGMPLYKYISNRLLTAFENLFLGVKLSEYHTGYRA
                     FSRKVLTDLPLLENSDDFVFDNQMLAQCVHFGFRIGEVSCPTKYFEEASSINFRRSVK
                     YGFGVLGTTLQFALQRSGIIHLARFSDKGRKLETSHQTYYARRSETVRSA"
     gene            3068..4612
                     /locus_tag="VEJ00_09240"
     CDS             3068..4612
                     /locus_tag="VEJ00_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01385.1"
                     /translation="MSDPPQNPAGLTSRNTDSSATALAVLLLLTVVAFLLMGYHPGLE
                     DDAYYLAAIKHNLNPFLFPHDAEFFALQFQATAYDKLVAVFVRVTHLPLAWTLFLWQF
                     AAIFLILHGCWRISRRCFADRPAQWGAVTMIAVLLALPVSGTGINLVDQYLHPRSLAT
                     AAIAAAVVEVLDRRLWLAGILLAFAFANHALMASFGISFCFFLWWNLGRAGRRRSAPL
                     ASIALLAPLGWIFEPSSDAWRQAASTRSFYYLSHWQWYEWLGVFAPLVLIYASLHLLR
                     RGGDSHDRDSVLRSFVASLFYYGVFQTIVGLIIMLPPRLERLRPFEPMRYLHLLYLFF
                     FLIVGALLGRYLLGRHIYRWVLLFVPLGSGMFYAQRQMYPASQHLELPGRNPANAWVQ
                     AFTWIRQNTPVDSYFALDPLYMPLPGEDFHGFRALAERSAMADFDKDGGMAARVPRLA
                     PRWLKEVTALKDWRNFQGGDFAKLKNEFGVNWVVLSRADADFSSQELGTNCPYRNATV
                     AVCRVE"
     gene            4732..>5322
                     /locus_tag="VEJ00_09245"
     CDS             4732..>5322
                     /locus_tag="VEJ00_09245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXX01386.1"
                     /translation="MTFSRLGETPELSIVVPCYNEDKVLLETHRRLSAVAAEIGLPFE
                     LVYIDDGSRDDTPSVLANLQMSDPRVRVVVLSRNFGHQVAITAGLNHSRGRAVVVIDA
                     DLQDPPEVIPEMIARWREGYEVVYGTRRRRDGETAFKTKTAEWFYRLINRISEIPIPL
                     DTGDFRLLDRKAVDALASMPERDRFVRGMVSWVGFRQ"
BASE COUNT          967 a         1558 c         1681 g         1116 t
ORIGIN      
        1 ttccatttgg cgcccggccc ggcggaaagc ttgaaaggca ccgccgccct tcaagacaga
       61 aaggttgcac ctgagtatat accgctcgcc ggctgttgga acgatgaaac cagaggccga
      121 cgagcgcagg ttaccgcggc gggaacggcc ttccggggcc tggtttggcc gctgctcgcc
      181 gcttcgggcc tcaccgacgg cctgatgtat actcggacac tcctggtacc cgttcggtat
      241 ccagatccgc gccaatcatt gcctgaggag atccgccatg cagagccgca tcaacggcac
      301 caccatgcca gtgctggaat tcatcctgga accgaatgag tcgatcattt cggaagccgg
      361 agaactgtcg tggatgagca gttcgatcca gatgacgacc cacacgcagt ttgccggcgg
      421 gggaggaatt ttcggtgtgc tcaagcgggt ggcgggtgga ggttccctgt ttatgaccga
      481 atatcgcgct gtgggtgcgg ccggggagct ggcattcgcg accaagttgc cgggccatat
      541 cgtaccggtc gaagtcgctc cggggcacga gtacatgatt caccgccatg gattcttgtg
      601 cgctacttct cagattcaga tcggggtggg cttccaacag tcgctgggcg cgggcatctt
      661 cggcggcgac ggctttctgc tgcagcgggt cagcgggcag gggacggcat ggctggagct
      721 ttccggtgaa ttggtcgtgc gcgatctgca ggcgggtgaa acgctgcggg tgcatcccgg
      781 acacgtgggc gcgtttcaat cgagcgtttc gtttcagatc acgaccgtgc cggggatcaa
      841 gaacatgatc ttcgggggcg acgggatttt tctggcggcc ctgactgggc cggggagaat
      901 ctggctgcag acgctgccca ttgccaagct ggcgcacgcc attgaccatt atttgccgca
      961 tgaagtgcgg cggcagacag tggaaggcgg cgtggtcggc gggattgtcg gatcgattct
     1021 ggatgggatg aaatgaaacg ctcgatgaat cgtcgaccgt gccttttcgc cgcgctgatg
     1081 tttctcgcgc catttgtcgt tgcgcaggag gcgcccaggt acccgatcac cgagggcgat
     1141 ttcgttgccc acgatttcaa gttccaatcg ggggagcaac tcgccgaact gcggctgcac
     1201 taccgcacgc tggggacgcc ggcgcgcgac gccgacggta acgttacgaa tgcagtgctc
     1261 ctgctgcacg gaaccggcgg atcgggagcg cagtttctgg cgccgcagtt tgccgacgag
     1321 ctatacgggc cgggtcaacc gctcgacacg aaccgttact tcatcatcgc ggccgacggg
     1381 atcgggcacg gcaagtcgtc gaaaccgagc gacgggatgc atgcgcattt cccgcagtac
     1441 gactacgacg acatggtcgc ggcgcagcat ctgctgctga ccgacgggct gaaagtacag
     1501 catctgcggc tgattttcgg cacttcgatg ggctgcatgc actcgttcgt gtggggtgag
     1561 gcgtatccgg attttatgga cgcgctcatg cccatggcct gcctgccagt gcagatcgcg
     1621 ggccgcaacc gcctgtggcg gaagatgctg atggacgcga tccgagagga tcctgactgg
     1681 aaagacggag agtacaagga ggaaccggca cgggcgctgc gcaccgcgca ggatctgctg
     1741 atcctcgcag gcagcgcgcc ggtgctcatg cagaagactc tgcccacgcg cggtgcggcg
     1801 gacaaatatc ttgccgatgc ggaggggcgg ccgggcaccc cgccggacgc gaatgacctg
     1861 ctgtatcagg tgaatgcctc gcgcaactat gatccgtcgc cgcaactcga gaagatcacg
     1921 gcgcgcgtga tgtggatcaa ctccgccgat gacttcatca acccgccgga gctggggatc
     1981 gcagaaaaag agtgcaaacg cctgaaaaac gggacattcg tgctgctgcc catcagcgag
     2041 aagacgcatg ggcacgggac acacacatgg gccgtagcat ggaaggaata cctggtccag
     2101 accctggcac tagcgggtca ttgatcgcgg ccatgttgtg cgagccatgg gtgagcaacg
     2161 tacgcggcaa cagttcttaa cttcccgtca aacatctcgg atacactagt ggggccccgt
     2221 ttgggtctag cgcaatgatc aacggcaaac gcattgcggt cgttatgccc gcctacaacg
     2281 ccgagaagac gcttgaggcc acggtagggg aggtccccga actgaccgac atccgcattc
     2341 tggtcgacga ccacagctcg gaccagacag tggaactggc gcggcgcctg ggattgcttg
     2401 tcttccagca tgaccgcaac tacggctacg ggcgcaatca gcagacctgc tatcgcgagg
     2461 cgctggaggc gggcgcggac gtggtcatca tgctccatcc cgactaccag tacacaccgc
     2521 tgctgatcac cgcgatggcg agcatggtcg cctacgacgt ctacgacgta gtgctgggct
     2581 cgcgcatcat cggtggccgg gcgctgcggg gcggcatgcc gctgtacaaa tacatttcga
     2641 accggctgct gaccgcattt gagaatctgt ttctgggcgt gaagctgtcg gagtatcaca
     2701 cgggataccg cgccttcagc cgcaaggtac tgaccgatct gccgctgctc gagaactccg
     2761 acgactttgt cttcgacaac cagatgctgg cgcagtgcgt gcacttcggc tttcgcatcg
     2821 gggaagtttc ctgtccgacg aagtatttcg aagaggcctc gtcgatcaat ttccgccgca
     2881 gcgtgaaata cggtttcggc gtgctgggca cgaccctgca gttcgcgctg cagagaagcg
     2941 gcatcattca tctcgcgcgg ttcagcgaca aaggacgcaa gctcgagacc agccaccaga
     3001 cgtactatgc gcgccgcagc gaaaccgtgc gctcggcctg agcaacctgc gctggtcttc
     3061 tcttcgaatg tccgatcccc cgcaaaatcc ggcaggcctc acatcgcgca atacggattc
     3121 ttctgcaacg gcgctggcgg tactgttgct gctaacggta gttgccttcc tgttgatggg
     3181 ttaccacccc ggtctggaag acgatgccta ttacctggct gcgatcaaac acaacctgaa
     3241 tcctttcctg tttccgcacg atgcggagtt tttcgccctg cagtttcagg cgactgccta
     3301 cgacaagctg gtcgcagtgt ttgtgcgcgt gacgcacttg cctctggcat ggacgctctt
     3361 cctctggcag ttcgcagcca tttttctgat tctgcacggg tgctggcgaa tcagccggcg
     3421 gtgttttgct gaccgtcccg cgcaatgggg ggcggtgacg atgatcgccg tgctgctcgc
     3481 gctgccggtg tcgggaacgg gaatcaatct ggtcgatcaa taccttcatc cgagaagcct
     3541 ggcaacggca gcgattgcgg cggcagtggt cgaggttctc gaccggcggt tatggctggc
     3601 tggaatcttg ctggcctttg ctttcgccaa ccacgccctc atggcaagtt ttggaatttc
     3661 tttttgtttt tttctgtggt ggaacctggg gagagctggc cggcgccgtt ccgccccctt
     3721 ggcatcgatc gcattgttag cccctctggg gtggatcttc gagcccagtt cggacgcatg
     3781 gcggcaggcg gcttcgacgc gcagtttcta ctacctcagc cactggcagt ggtatgaatg
     3841 gctgggcgtg ttcgcacccc tggtgctgat ctacgcctct ctgcatttgt tacgacgcgg
     3901 tggagactcg catgatcgag actctgtgct gcggtcgttt gtagcaagtc ttttttacta
     3961 tggcgtgttc cagacgattg tcgggctgat catcatgctg ccgccccgcc tggagcgcct
     4021 gcggccgttt gagccgatgc gctatctgca tctgctctat ctgttcttct ttctcatcgt
     4081 gggcgccttg ctgggccgtt atctgcttgg gcggcatatc tatcgatggg tcctgctttt
     4141 tgttcccctc ggctccggca tgttttacgc gcagcgccag atgtatccgg cgagccagca
     4201 tctggagttg ccggggagga accccgccaa tgcgtgggtt caggctttca cctggattcg
     4261 gcagaacact ccggtcgaca gctacttcgc gctggatccg ctgtacatgc cgcttccggg
     4321 cgaggatttc cacggctttc gcgctctggc cgaacgcagc gcgatggccg acttcgacaa
     4381 ggacggcggc atggcggcgc gtgttccgcg gctggccccc cgctggctga aagaggtgac
     4441 tgcgctgaag gactggcgga atttccaggg gggcgatttc gcgaaattga agaacgagtt
     4501 cggggtgaac tgggtcgtgc tctcgcgcgc ggatgcggat ttcagcagcc aagagttggg
     4561 gacgaactgt ccctaccgaa acgccacggt cgcggtgtgc cgagttgagt aagtggtgac
     4621 ggcaggtcct gccttgctcc gaatcgcgcc cgaccctaca atcggtttac cgacctttcg
     4681 cagtgcgatc accgttttcg atcggcagca ggatttgtgg agcctcatgg aatgactttt
     4741 tcgcgccttg gagaaacacc cgagctctcg atcgtagtgc cttgctacaa cgaggataag
     4801 gtattgctcg aaacccaccg ccggctttcg gcggtggcgg cggaaatcgg gttgcccttc
     4861 gaacttgtct acatcgacga cggcagtcgc gacgacacgc cctctgtgct cgccaacctt
     4921 caaatgtcag acccgcgcgt tcgcgtcgtg gtcctctccc ggaacttcgg gcaccaggtg
     4981 gcgatcacgg ccggactcaa ccatagccgc ggccgggccg tagttgtgat cgacgccgac
     5041 ctgcaggacc cgcctgaggt tatcccggaa atgattgccc gctggcgtga ggggtacgaa
     5101 gtggtctacg gcacgcgccg gcgccgggac ggggaaaccg catttaaaac gaagaccgcc
     5161 gagtggtttt atcgcctgat caaccgtatc tccgagattc ccattccgct ggacaccggc
     5221 gatttccgcc tgctggatcg taaagcggtg gatgctctgg cctccatgcc agaacgtgac
     5281 cgcttcgtcc gcggcatggt gagctgggtg ggattccggc ag
//
LOCUS       DASPUL010000381         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1293650, whole genome shotgun sequence.
ACCESSION   DASPUL010000381 DASPUL010000000
VERSION     DASPUL010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293650"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VEJ00_09250"
     CDS             complement(<1..281)
                     /locus_tag="VEJ00_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXX01387.1"
                     /translation="MWIVALALRRPYTFVVMALVLLLLTPLVVVRMPVDVLPDINIPV
                     ISVVWRYDGLAPDEMADRIVSNAERGLTITVNDIEHIESQSLNGIGVIK"
     gene            559..2328
                     /locus_tag="VEJ00_09255"
     CDS             559..2328
                     /locus_tag="VEJ00_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HXX01388.1"
                     /translation="MQWTQIYDPFGNWWLSTLVAALPIIVLFALLAGLKVKPHWCAIA
                     GAATAVLVAILFFKMPPLLAGLSFAYGVAFGLLKIAWIVLAAVYLYDISVETGQFEIM
                     KESIAGITADRRLQVLLVAFCFGAFIEGAAGFGAPVAIAGAFMIGLGFKPFHAAALNL
                     IANTAPVAWGAIGTPVHTLAAVTALPESDLSAMIGRILPLTAVIVPFWLVRTMVSWKE
                     TFEVFPAILVVGGSFALTQFVWSNYVDSNLVDIMGGVLSIIATLIFLRFWKPKKIWRF
                     DYDDGGSALPPPEAEISDQMGAEWTAKEYDRFASARTHTAGQVLKAWMPFAILSLFVL
                     LWGLPRVKLAMNQATTPAFRVVLPDGKVRPGPPGWDVPYLHNAISRAVPVVAKPTPEA
                     ARYDFNWLSATGTAVFFAAAVSGLLLGLSPGRLLKIFWRTLVRMRLAMIAISFMLGLG
                     YVTRYSGLDAILGLAFTRTGWLYPFFGTFLGWLGVALTGSDTSANALFGSLQRITSQQ
                     LGIDPVLMCAANSAGGVMGKMVDAQSITIATAATEQVGNEGIVFRFVAWHSVALCSIV
                     GVMVLLYAYVFRSAVPHGLLFVK"
     gene            2446..2523
                     /locus_tag="VEJ00_09260"
     tRNA            2446..2523
                     /locus_tag="VEJ00_09260"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2481..2483,aa:Val,seq:gac)
BASE COUNT          473 a          770 c          785 g          625 t
ORIGIN      
        1 atcttgatca ctccgatgcc gttgagcgac tgcgactcga tgtgctcgat gtcgttgacg
       61 gtgatggtca gtccgcgctc ggcgttggag acaatgcggt cggccatttc gtcgggggcc
      121 agcccgtcat acctccaaac cacgctgatc accggaatgt tgatgtcggg taagacatca
      181 accggcatgc ggaccacgac cagcggcgtc aggagaagca gcaccagcgc cattacgaca
      241 aacgtatagg gacgcctgag ggctaacgct acaatccaca tcgatcaact gttccaaccc
      301 ggcagggtga agatcttggg cagtgtccgc cgtgcggtga agatacggct gaaatacggt
      361 gcccagacta acccagtata tgagaaatgg gctgccgatg cagagtgcgg cgggccgcat
      421 aaagcccgat tctgccgcac tgttgggggt gctaggatat cggcgcactc cggcccgcca
      481 ggcggggagg ctggcgcgga gtgctctcgt gtgtatctgt ctggtcccgt ttcgattccc
      541 ccttgcaagg agacgatcat gcagtggacc cagatctatg atccattcgg aaattggtgg
      601 ctctccacgt tggtagctgc tctcccgatc atcgttctct tcgctctgct ggccggcctg
      661 aaggtgaagc cgcactggtg tgcgattgcc ggcgctgcaa ctgcagtgct ggtcgcgatt
      721 ctctttttca aaatgccgcc gctgctggcg ggattgagtt ttgcctatgg agtcgcattc
      781 gggctcttga aaatcgcctg gatcgttctg gccgcggtct acctctacga catttcggtt
      841 gagaccgggc aattcgaaat catgaaggaa tcgatcgccg gaatcaccgc tgatcggcgg
      901 ctgcaggttc tgctcgtggc tttctgcttt ggcgccttca tcgagggagc ggcgggattc
      961 ggcgctccgg tcgccattgc cggcgccttc atgatcgggc tcggcttcaa gcctttccac
     1021 gccgccgcgt tgaacctgat cgccaacacc gcgcccgtcg cctggggagc gatcggcacg
     1081 ccagttcaca cgcttgcggc cgtgaccgcg ctgcccgaat ctgatctgag cgccatgatc
     1141 ggccgcattc tgccgcttac cgccgtgatc gtcccttttt ggctggtcag aacgatggtc
     1201 agctggaagg agacgttcga agtttttccc gcgatcctgg ttgttggcgg ttccttcgct
     1261 ttgacgcaat tcgtctggtc gaactacgtc gacagcaacc tcgtcgacat tatgggaggc
     1321 gtgctctcga tcatcgccac gctgatcttc cttcgcttct ggaagccaaa gaaaatttgg
     1381 cgtttcgact atgacgacgg gggatctgct cttccgccgc ccgaagcaga gatatcggat
     1441 caaatgggag ccgagtggac cgcgaaggaa tatgacagat ttgcctctgc acgtactcac
     1501 accgctgggc aggttctaaa ggcctggatg cctttcgcca tcttgtcgct gttcgtcttg
     1561 ctctggggct tgccccgggt caagctggcc atgaaccagg ccacgacgcc tgccttccgg
     1621 gtcgtgcttc cggacgggaa agtgcgcccc ggtccgccgg gatgggatgt gccttacctg
     1681 cacaacgcta tctcgcgcgc cgtgccggtg gtcgccaagc cgactcccga ggcggcccgc
     1741 tacgatttca actggctctc ggcaacgggc acagctgttt tttttgccgc cgctgtttcc
     1801 ggcctgttac tgggtctgag tccggggcgt ttgctgaaga tcttttggag aacgctggtg
     1861 cgcatgcgcc tggccatgat cgcgatctca ttcatgctgg gcttaggcta tgtcacgcgc
     1921 tactcgggtt tggacgccat attggggctg gcgtttaccc gcaccggatg gctgtatcca
     1981 ttctttggca cattcctcgg ttggctggga gtcgcgctta ccggcagcga cacctccgcc
     2041 aatgcgctgt tcggcagcct gcagagaata acttcgcagc aacttggaat cgatcctgtg
     2101 ctcatgtgcg cggccaacag cgccggcgga gtgatgggga agatggtcga cgcgcaatcc
     2161 attaccatcg ccaccgctgc caccgagcag gtgggcaacg agggaatcgt tttccgcttt
     2221 gtggcctggc actcggtggc tttgtgctcc atcgtcggag ttatggtcct gctctatgcc
     2281 tatgttttcc ggtctgcagt gccacatggg ctgctgttcg tgaaatagtt catgaaatag
     2341 aacgtggatc actcagagcg cagcctccgg tcgagtgtcg caagggctgg gcgtttgccg
     2401 gaattctcct ctacccgtag aatgaggtca ctgccgcttg catgtaggcc cgtagctcag
     2461 ttggtccaga gcgcttcctt gacacggaag aggtcgatgg ttcgaatcca ttcgggccta
     2521 ccatcatttc catagggata gagagaatcc ccaaaattct ctagcccgtg ctcagcccgt
     2581 gatcctaaga ataattaccc ctctgacggc cggaatttcc attgacattg cttccatgtt
     2641 tctgcaatca tgg
//
LOCUS       DASPUL010000382         5448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1715859, whole genome shotgun sequence.
ACCESSION   DASPUL010000382 DASPUL010000000
VERSION     DASPUL010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715859"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VEJ00_09265"
     CDS             complement(<1..228)
                     /locus_tag="VEJ00_09265"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXX01389.1"
                     /translation="MTLSLPPFTPAVIWLVGINTAVYLLWLVLGVFRLPVDDFLLDYL
                     ALTPVQVVSHGWLWQLVTYSFLHHEFWHWFGN"
     gene            complement(282..1034)
                     /locus_tag="VEJ00_09270"
     CDS             complement(282..1034)
                     /locus_tag="VEJ00_09270"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXX01390.1"
                     /translation="MLRFSEWMKAMTVAVVLAAEFACPAQDAGAGTGWTADPQIVSAL
                     KQVSAQRIEATVEKLVSYYNRSTLSSADPDLLKAERGITAARLWIVSEYERYSKECGG
                     CLDVETHKFVQEPGERVPNPTEIVDVYAVLKGTDPANAKRIVLVTGHYDSRNSDPLDA
                     KGFAPGANDDASGTAVSLRLGTGVCRWCRRRNDEKPFEIQTKIPILAAKDAARMGHPQ
                     YGAGRVSGPTEAPLLAQRAREKWGTRCGARVE"
     gene            1198..1398
                     /locus_tag="VEJ00_09275"
     CDS             1198..1398
                     /locus_tag="VEJ00_09275"
                     /inference="COORDINATES: protein motif:HMM:NF044773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01391.1"
                     /translation="MVQCPECETNLDVEEEEVDEGEVISCPECGTDFEVITTSPLELK
                     AVEEGYEEEEEESEEESEDGDY"
     gene            1388..1801
                     /locus_tag="VEJ00_09280"
     CDS             1388..1801
                     /locus_tag="VEJ00_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01392.1"
                     /translation="MTTDLARRLSLAGFLLAATILPSFSAHPLHAASAHPKEKPYALI
                     FGTVWGPDDLPVYGVTVKIRRATDKPRKVRWEVQSDHHGEFAQRVPVGECDYILSADL
                     KGVKSSDGQPLRLLKEVTVHIYNDEREDTGLHLSH"
     gene            2153..2524
                     /locus_tag="VEJ00_09285"
     CDS             2153..2524
                     /locus_tag="VEJ00_09285"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXX01393.1"
                     /translation="MNRKRWVSAAVALACALLVVLGLLAAASPEKKEKQVGRLVFGKV
                     LDPQDNPLPDAVVYLTNTRTRAIKTYIVSTDGTYRFPALSTAIDYEIYAQYKGKKSDT
                     KSVSQFDDRPQVYIDLKIDTR"
     gene            2769..3380
                     /locus_tag="VEJ00_09290"
     CDS             2769..3380
                     /locus_tag="VEJ00_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01394.1"
                     /translation="MRRIVTTMRTSRKATSLTVKVALVCFALLCAAATLLAQGGAMSP
                     YQGEKDGVTAGGKWMMFQSEDKMTGAKRARFELLSNNYFKEDPDYKPRVELVCEDGKY
                     KMADFNPGVRIRPDRPGFWGQPQIEVEVRVDDVHNFHGWNWRGRVLSMDKGTARGMIG
                     AQIFNIELPTPSGRQIAEFSPAGLNVDQVRQFCDLTPKKPSKD"
     gene            3619..4200
                     /locus_tag="VEJ00_09295"
     CDS             3619..4200
                     /locus_tag="VEJ00_09295"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXX01395.1"
                     /translation="MGSDLITGLALFLFAAGVFLYAVILYNGLIRLRNENDRAWANID
                     VLLKQRHDEIPNLVETVKGYMQHEQQTLLAVTQARAASMNAASIGQKALADLKMASAL
                     RGLFAVAENYPQLRANENFLKLQGRISELEERIADRREFFNDDVATYNSRIAQIPDVF
                     LASFMSLKPRQMFKVSDEDRKQVEVSFQRASGA"
     gene            complement(4401..>5448)
                     /gene="hemB"
                     /locus_tag="VEJ00_09300"
     CDS             complement(4401..>5448)
                     /gene="hemB"
                     /locus_tag="VEJ00_09300"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HXX01396.1"
                     /translation="ELRNMVSETRLTPDAFVYPMFVCPGEGVRKEVRSMPGVCNLSVD
                     EAVKEAREAHSLGVPSVILFGLPDKKDDVATGAWAEDGIVQQAARAIKREVPGLVLIG
                     DVCLCEYMSHGHCGIVKPTVPRSLGAAARNTVAVKGWNSSEEAIDALVEQAKRSAEAN
                     YEIVNDATLELLAKTSVSLANAGIDIIAPSDMMDGRVGAIRKALDEAGFINTPILSYA
                     AKFASGFYGPFREAADSAPQFGDRRSYQMDGANVREAVREIAADIEEGADMIMVKPAM
                     PYLDVIAAAHRRFDLPLAAYQVSGEYAMIEAAARNGWIERERVMMESLVSIRRAGASI
                     ILTYYAKEAARVLG"
BASE COUNT         1116 a         1884 c         1436 g         1012 t
ORIGIN      
        1 gttcccgaac cagtgccaga attcatggtg aagaaagctg taggtcacca gctgccacag
       61 ccagccatga gacacgacct gaaccggtgt cagggcgaga taatcaagca ggaagtcgtc
      121 cactggcagc cggaacacac ccagcaccag ccacagcaaa taaacagccg tgttgatccc
      181 caccagccag atcaccgccg gggtgaacgg gggcaggctc aacgtcatcg gttctctgct
      241 tcgcggcaca gtagacctct gctaatttac tcgatttgga tctattctac tcgggcgccg
      301 cagcgggtgc cccatttctc gcgcgccctt tgcgcgagaa gtggggcttc cgtcggcccg
      361 cttacccttc cagcaccgta ctgagggtgc cccatcctag ccgcgtcctt cgcggctagg
      421 atggggattt tcgtctggat ttcaaacggc ttctcatcgt tccggcgtcg gcaccaccgg
      481 caaactcctg tgcccagcct gaggctcacc gccgtaccgc tggcgtcgtc attggcgccg
      541 ggcgcgaatc ccttcgcgtc cagaggatcg ctgttgcgcg agtcgtagtg ccccgtgacc
      601 agcacgatcc gcttggcatt cgccggatcc gttcccttca gcaccgcata aacatcaaca
      661 atctcggtcg gattcggaac tcgctcgccg ggctcctgca caaacttgtg cgtctcgacg
      721 tcgaggcaac cgccgcactc cttggaatag cgctcgtact ccgacacgat ccacaaacgc
      781 gccgccgtaa ttccccgctc cgccttgagc agatccggat ccgcgctcga caaggtcgac
      841 cggttgtagt agctgaccag tttttcgacg gtggcctcaa tgcgctgtgc ggagacttgc
      901 ttgagcgctg acacaatctg cggatcggcg gtccatcccg tgccagctcc ggcatcttgc
      961 gccgggcacg caaactctgc ggccaacacg accgcgaccg tcatcgcttt catccattcg
     1021 gagaatcgca gcatgaccgt ctcgattcgc tctcgcagaa cccgtgggcg gcgcggcagg
     1081 acgcgaaggg taggggttcc tgcgagcccc gaaataccct acaggctgtg cctcttgacc
     1141 aaccgccggg aagaatctaa atattatcag gaggctctga aaggcagggg aggctttatg
     1201 gtgcagtgtc ccgagtgcga aaccaatctg gacgtcgaag aagaagaagt cgacgagggc
     1261 gaggtcattt cctgcccaga gtgcggcacc gacttcgagg tcatcactac cagtccgctc
     1321 gagttgaagg ctgtcgaaga aggctacgaa gaggaggaag aagagtcgga agaggaaagc
     1381 gaagacggtg actactgatc tggctcggcg cctttctctc gctggcttcc tgcttgccgc
     1441 aacgattctg ccgagcttca gcgcccatcc tctgcatgcc gcttccgcac accccaaaga
     1501 aaagccctac gccctgatct tcggaaccgt ctgggggccg gacgatctcc cggtctacgg
     1561 tgttacggtg aagatccgtc gcgcgaccga caagcccagg aaagtccgct gggaagtcca
     1621 atccgaccac catggcgagt tcgcccagcg cgttcccgtc ggcgagtgcg actacatcct
     1681 ctccgccgat ctcaagggcg ttaagtcgtc cgacggccaa cccctgcgcc tgctcaagga
     1741 agtcaccgtc cacatctaca acgacgagcg cgaggacacc ggcctgcacc tgtcccacta
     1801 atactctccc caagacgcgc ccaaaccgcg ccgtcatcct gatccccaag acccgctgtc
     1861 atcctgagcg aagcgaagga tctatgtacc ttgcttcgag ctggtaggac ccgaaggttg
     1921 tcgacggcgg tggcgcgatg ccggaacgat gccacccccg gtccctagtc cagtcgcgaa
     1981 gcgacgaaat tcattagccc ggcgcgtaag tgccgggaaa cggacccgga caatgtccgg
     2041 ctcccgtagg gacgccaccg attcccgcct ctcacatcct ccttccgaac caatttgcaa
     2101 gattggttcg aacctgcggc gactcccgcc agccgtgaga aaataaccca acgtgaatcg
     2161 caaacgatgg gtctccgccg ccgtcgctct ggcctgcgcg ctgctcgtgg tgctgggctt
     2221 gctcgccgcc gcctcgcccg aaaagaagga gaagcaggtc ggtcgcctcg ttttcggaaa
     2281 ggtcctcgat ccccaggaca accccctccc cgacgctgtt gtctacctca ccaacacccg
     2341 cacccgcgcc atcaagacct acatcgtcag cactgacggc acctaccgct tccccgccct
     2401 ctccaccgcc atcgactacg aaatctacgc ccaatacaag ggcaagaaga gcgacaccaa
     2461 gtccgtcagc cagttcgacg accgccctca ggtctatatc gacctcaaaa tcgacacccg
     2521 ttagtgtggc gcgggctctc tcgcccgcga agggggctaa atctgtcgcg gtcgccaccc
     2581 aggtcgcgtt tttctgcgac agagccttgc cttgagcgag agcagcgagc cgaaaggcgg
     2641 gaacggccgt accttttcgt gcaacttttt ccaccggttc cgggttcaat ataggtagag
     2701 aaatccgtct ccgcaggatt gtcttcagga cgatcgggca tgcaagatcg ttcttcagga
     2761 ggctcgccgt gaggaggatc gtgacgacca tgcgcacttc tcgtaaagca accagtctga
     2821 ccgtaaaggt tgctctcgtt tgtttcgcgc tgctttgtgc agccgcaact ttattggccc
     2881 agggcggtgc catgagccct taccagggcg aaaaagacgg cgtcaccgcc ggcggcaaat
     2941 ggatgatgtt ccagtccgag gacaagatga ccggcgccaa gcgcgcccgc ttcgaactcc
     3001 tgtccaacaa ctatttcaag gaagaccccg actacaagcc tcgcgtcgag ctggtctgcg
     3061 aagacggcaa gtacaaaatg gccgacttca accccggcgt ccgcattcgc cccgaccgcc
     3121 ccggcttctg gggccagccc cagattgaag ttgaggttcg cgtcgacgac gtccacaact
     3181 tccacggctg gaactggcgc ggccgcgtcc tttccatgga caagggcacc gcgcgcggca
     3241 tgatcggcgc gcagatcttc aatatcgaat tgcccacgcc cagcggacgc cagatcgccg
     3301 agttctcccc cgccggcctc aacgtcgatc aggtccgcca gttctgcgac ctgaccccga
     3361 aaaaaccgag caaggactaa aagcagggat caggggctag ggggcaggga tcagaacggc
     3421 aagacgatgt tcctaagggg tgctctccaa cttctgcatc ccggcgaagc cgaattgccg
     3481 ccagagcggc agctccatac attcgaggga tcttgcgttc ttttactgac ccctgacccc
     3541 tgatccctgg gccctggttc gccactcttc cgtcacttgc caccccgccg cctctgctgt
     3601 cacctttaag aagcctccat gggctccgac ctcatcaccg gcctggcatt gttcctgttc
     3661 gccgccggag tcttcctcta cgccgtcatc ctctacaacg gcctgatccg cctgcgcaac
     3721 gaaaacgacc gcgcctgggc caacattgac gtcctgctca agcagcgcca cgacgaaatc
     3781 cccaacctgg ttgaaaccgt caagggatac atgcagcacg agcagcaaac cctgctcgcc
     3841 gtcacccagg cccgcgccgc ctcgatgaac gcggccagca tcggccagaa agccctcgcc
     3901 gacctgaaaa tggccagcgc gctgcgcggc ctgtttgctg tcgccgaaaa ctatccccag
     3961 ctcagagcca acgaaaactt cctcaaactc cagggccgga tcagcgagct cgaggagcgc
     4021 atcgccgacc gccgcgaatt tttcaatgac gacgtcgcca cctacaactc ccgcatcgcc
     4081 cagatccccg acgttttcct cgccagcttc atgagcctca agccccgcca gatgttcaaa
     4141 gtctccgacg aagaccgcaa gcaggtcgaa gtcagctttc agcgcgcctc cggcgcgtaa
     4201 tgacacttct ggaaccgcaa cgaacgggaa gggcacggct tcagccgtgc cgctaaacgc
     4261 taaaaaggag atggcgcttt agcgcctgag gtatgccttt gcttacgatc cgcccccgga
     4321 gagttgaaga agtcctagct actgacaacc caatcggtcg cggcccttca ttcaacattc
     4381 aaaaatcaaa aatctgagat ctaccccaat accctcgccg cttctttcgc gtaatacgtc
     4441 aaaataatgc tcgcccccgc ccgccgtatg ctcaccagcg attccatcat cacccgttcg
     4501 cgctcgatcc atccatttcg cgccgcggct tcgatcatcg cgtactcgcc cgatacctga
     4561 tacgccgcca gcggcagatc gaagcgccgg tgcgccgccg caatcacgtc gagatacggc
     4621 atggccggct tcaccatgat catgtccgcg ccttcttcaa tatcggccgc gatctcccgc
     4681 accgcctcgc gcacgttcgc tccatccatc tgatacgacc gccggtcgcc gaactgcggc
     4741 gccgaatccg ccgcttcgcg gaacggccca taaaatcccg acgcaaactt cgccgcatac
     4801 gacaaaatcg gcgtgttgat gaaccccgcc tcatccagag ccttgcgaat cgctcccacg
     4861 cgcccgtcca tcatgtccga cggcgcgatg atgtcgatcc ccgcgttcgc caacgaaact
     4921 gaagtcttcg ccagcagttc cagcgtggcg tcgttcacga tttcgtaatt cgcttctgcg
     4981 cttcgcttgg cctgctcgac cagtgcatca atcgcctctt cgctcgaatt ccatcccttc
     5041 accgcgactg tatttcgggc cgcggcgccc agcgatctcg gaaccgtggg tttcacgatc
     5101 ccgcaatgcc catgcgacat gtactcgcac aggcagacgt cccctataag gaccagcccc
     5161 ggcacctcgc gcttgatcgc gcgcgccgcc tgctgcacga tgccatcctc tgcccacgcc
     5221 ccggtcgcca cgtcatcttt tttgtcgggc aggccaaaca ggatcaccga cggcacgccc
     5281 agcgagtgcg cttcgcgggc ctctttcacc gcctcgtcca ccgaaagatt gcacacgccc
     5341 ggcatcgagc gcacttcctt gcgcacgccc tcgccggggc acacaaacat cgggtagaca
     5401 aacgcgtccg gagtcagccg cgtctccgac accatattgc gcagctcc
//
LOCUS       DASPUL010000383         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_903111, whole genome shotgun sequence.
ACCESSION   DASPUL010000383 DASPUL010000000
VERSION     DASPUL010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903111"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VEJ00_09305"
     CDS             <1..244
                     /locus_tag="VEJ00_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01397.1"
                     /translation="FLVRQADLGDGTWDITEMDLSITGKILFFKSISMVSDEVFSDFR
                     RVPDNLTFAQGVELLKSEREKLARSEAPSEAKRTAQ"
     gene            complement(245..1354)
                     /gene="menC"
                     /locus_tag="VEJ00_09310"
     CDS             complement(245..1354)
                     /gene="menC"
                     /locus_tag="VEJ00_09310"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HXX01398.1"
                     /translation="MKIEAVTLREIHMPLVHFFETSFGRTYSRRILLVTVHCEGVDGW
                     GESVAGEDPFYSSEWIESAWPTLTKYLIPTLLGRRLESARECPALLTRVRSHRMAKAA
                     LENALWDAEAIQKQQPLWKLLGGVRSQIPCGVSIGIQDSVEQLLEKIQTELAAGYRRI
                     KVKVKPGWDVNVLERIRARWADITLSCDANSAYTLDHVEHLRKFDQFNLLMIEQPLWH
                     DDIYYHARLQKELRTAICLDESIVSGRAAAFAVESGACRIINVKVGRVGGFTEAMKVH
                     DIARANNIPVWCGGMLETGIGRAHNIAVSTLPNFSLPGDVSASKRYWREDIIEPDVEV
                     SPQGMIAISENPGRGYEVKPDLIEKLTVRKETIRA"
     gene            1465..>2153
                     /locus_tag="VEJ00_09315"
     CDS             1465..>2153
                     /locus_tag="VEJ00_09315"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXX01399.1"
                     /translation="MRIFKPLLGSILAALIALPPTAYAQNKAATVPTVKHVLLISVDG
                     LHALDLANFVATHGDSTLAFLSSQGITYTNASTTTPSDSFPGLASLVTGGSPVTSGLW
                     YDDTYNRALSPPRHTDGLGNPGGPCPGKIGTNVAWDESVDFDLTKLNGGGGINPNFLV
                     RDPNNNCATLMPHQYLRVNTIFEVVKANGGTTAWTDKHPSYEWTNGPSGKGVDDFYGP
                     EINSTPIALPQ"
BASE COUNT          436 a          658 c          614 g          445 t
ORIGIN      
        1 cttcctcgtg cggcaggcgg atctgggcga cggcacatgg gacatcaccg aaatggatct
       61 cagcatcacc gggaagatcc tgttcttcaa gagcatcagc atggtttccg atgaagtgtt
      121 cagcgatttc cggcgggtac ctgacaacct gactttcgcg cagggagtgg agctgctgaa
      181 atcggagcga gagaagctgg cgcgatctga ggctccctcg gaagcgaaga gaactgcgca
      241 atagctaggc gcgtatcgtt tccttccgca ccgtgagttt ctcgatcaag tctggtttca
      301 cttcatagcc tcggcccgga ttttcgctga tggcgatcat cccctgcggc gaaacttcca
      361 catccggctc gatgatgtct tctctccagt agcgctttga ggcggagacg tcgcctggaa
      421 ggctgaagtt tgggagggtt gaaacagcga tattgtgggc gcggccgatc ccggtctcca
      481 gcatgcctcc gcaccagacg ggaatgttgt tggcgcgggc aatgtcgtgg accttcatcg
      541 cttcggtgaa accgccgacc cggcccactt tgacgttgat gatacggcag gcaccgcttt
      601 cgacggcgaa ggccgcggca cgcccgctga cgatggactc gtcgaggcaa atcgcggtgc
      661 gcaactcctt ctgcagccgg gcgtggtagt agatgtcgtc gtgccaaagc ggctgctcga
      721 tcatcagcag attgaactga tcgaacttgc gcaggtgctc gacgtgatcg agcgtgtagg
      781 cggaattggc atcgcagctg agcgtgatgt cggcccagcg cgcgcgaatg cgttccagca
      841 cattgacatc ccagccgggc ttgaccttaa ctttgatgcg gcggtatccg gcggcgagct
      901 cggtttggat cttctccagc aactgctcga cggaatcctg aataccgatg gaaacgccgc
      961 agggaatctg agaacgcacc ccgccgagca acttccagag tggttgctgt ttctggatgg
     1021 cttcggcatc ccacaatgcg ttttcgagag cggctttagc catgcgatgc gagcgcactc
     1081 tcgtgagcaa ggcggggcac tcgcgcgcgg actccagacg tcttccaaga agcgtgggaa
     1141 tcagatactt cgtcagcgtg ggccaggcgc tttcgatcca ttcgctgctg taaaagggat
     1201 cttctccggc cacgctctcg ccccagccgt caacaccttc gcagtgaacc gtgaccagga
     1261 gaatacggcg gctgtaggtg cggccgaagc tcgtctcgaa gaagtggacg agcggcatgt
     1321 gaatctcgcg cagcgtgacc gcttcaatct tcatagcctg cttacctgaa cttcttttta
     1381 caatctattc atctgcggtt tacgaagggc ctgagcctgc tctttagaat cctcgcacag
     1441 tccaaccttt ggaggaattc cttaatgcga atcttcaagc ctcttctcgg ctcaattctg
     1501 gcggcgctga ttgcacttcc gccgacggct tacgcgcaaa acaaggcagc caccgtgcct
     1561 accgtgaaac acgttctact gatcagcgtc gacggtctgc acgccctcga tcttgccaac
     1621 ttcgtagcta cccacggcga ctcgaccttg gcgttcctca gcagtcaggg tatcacctac
     1681 accaacgcgt ccacgaccac accctccgat tcgtttcccg gactggcctc actggtcacg
     1741 ggcggatcac ccgtcaccag cggcctctgg tatgacgaca cctacaaccg cgctctctca
     1801 ccgccgcggc acacggatgg actcggaaat cccggcggtc cgtgccccgg caagattggc
     1861 accaacgtgg catgggatga gtcggtcgac ttcgacctga ccaagctcaa cggcggcggc
     1921 gggatcaacc ccaacttcct ggtgcgcgat cccaacaaca attgcgcgac cctcatgccg
     1981 caccagtatc tgcgggtgaa cacgatcttc gaagtggtga aggccaatgg cggcacgacc
     2041 gcctggaccg acaagcaccc ctcctatgaa tggacgaacg gacccagcgg caaaggcgtg
     2101 gacgacttct atggtcccga gatcaattcc actccgatcg cgctgccgca att
//
LOCUS       DASPUL010000384         3692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1294024, whole genome shotgun sequence.
ACCESSION   DASPUL010000384 DASPUL010000000
VERSION     DASPUL010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294024"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /gene="rpoN"
                     /locus_tag="VEJ00_09320"
     CDS             <1..948
                     /gene="rpoN"
                     /locus_tag="VEJ00_09320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HXX01400.1"
                     /translation="LKEALEVVRLLDPPGVACRDLRECLLYQLRYHQAQLALHKNGNG
                     TEQVLQDAVAVVDQHLRGLQNKQYKEIGKAIGRPVEAVEQALHYIRTLDPRPGLRYNK
                     VQARLIEPDVAFVKHGDAWLVIMNDEDLPQLRLNPAYRKLVTRDGTNDKTTRDYVKER
                     YKSAIQLIKNIEQRKQTITKVCYSIVARQQDFLERGIDQLKPMMIKEVAEEIGVHPST
                     VSRAVANKYAHTPQGVFELRYFFSESVQGPEGGNTSLLILKRRVKKLIDEEDPSRPLT
                     DEQITRILQSQGIQVTRRTVAKYREDMRIPSTHQRRVKK"
     gene            1096..1704
                     /gene="raiA"
                     /locus_tag="VEJ00_09325"
     CDS             1096..1704
                     /gene="raiA"
                     /locus_tag="VEJ00_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HXX01401.1"
                     /translation="MNVEYTGRHYEVTPTIRKEVETGLTKIRKILGDRFDTKVILAVE
                     KHRHKAEITISPRNGPLVGLAQARDMMIAVNDALEHLEKQAVKYKARWETKKRAARKT
                     EEVNKWNGHSTQAEVHTAVGLSEKTAVPVMVHKFPAVAKTTEVHLVRSEDAVAMRPMT
                     LEEAIKEAHFRDRDVFVFRDPKGKVMVLHRKRDGKMELIEAP"
     gene            1962..2393
                     /locus_tag="VEJ00_09330"
     CDS             1962..2393
                     /locus_tag="VEJ00_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01402.1"
                     /translation="MKKLFGLVVLLLCLAPALFAQEHYTEGPIWRVELIRVKPTHMDE
                     YLTSLRQSTKPLIEEEKRQGVIMDYKVFLKETKNSPEDWDICVAIEFKNHAALDGLAA
                     KGEMARDKILGGKQAAQQLGEKRAEIREIISSELLQEIFLK"
     gene            complement(2473..3327)
                     /locus_tag="VEJ00_09335"
     CDS             complement(2473..3327)
                     /locus_tag="VEJ00_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01403.1"
                     /translation="MKRPWQTVAILLLAVILPADLSWAQDLVQASRPDGAQTPLRVYA
                     PQTTGCPPLAVISPGAGGSENGYSYLAKALQDDGWRAIVMGHQESGGAALRSDVRQSR
                     GIKRGLQQLVNDPAAYKARLLDIAAALSWAESACKPPFVALLGHSMGARTVMVEVGAS
                     NTLDVKGMDRFNAYVALSPDGPGKMFPENAWSGVRKPMLILTGTRDQALDGDWKTRTI
                     PYESMPTGCKWLGVIDRATHMNFAGIGFAGSTEKLVLLETKAFLDGLRGGKCGAPVPS
                     AGITVKSK"
     gene            3502..>3692
                     /locus_tag="VEJ00_09340"
     CDS             3502..>3692
                     /locus_tag="VEJ00_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01404.1"
                     /translation="MIRTRRSCTVLFTLMSSLALGRGRLAHAQTASLPPKLSVHWEEL
                     TAADFREGIRRSQGTCLLP"
BASE COUNT          781 a         1047 c         1155 g          709 t
ORIGIN      
        1 ctgaaggagg cgctggaggt ggtgcggctg ctggatcctc cgggtgtggc ttgccgcgat
       61 ttgcgagagt gtttgctcta tcagcttcgt tatcaccagg cgcaacttgc cttgcacaag
      121 aacggcaacg gcacggagca ggttttacag gacgcggtcg cagtggttga tcagcatctg
      181 cgcgggctgc agaacaagca gtacaaagag atcggcaagg ccatcgggcg tccggttgaa
      241 gcggtcgaac aggcgttgca ctacattcgc acgctcgatc cccggccggg gttgcgctac
      301 aacaaagtgc aggcgcggct gattgagccc gatgtggctt tcgtgaaaca cggtgatgcc
      361 tggctggtga tcatgaatga cgaagacctg ccgcagcttc gtctgaatcc cgcctaccgc
      421 aaactggtga cgcgcgacgg aacgaacgac aagactacgc gcgattacgt gaaagagcgc
      481 tacaagagcg cgatccagct gatcaagaac atcgagcagc gcaagcagac catcaccaag
      541 gtctgctatt cgatcgtggc gcggcagcag gactttctgg aacgtggcat cgatcaactc
      601 aaacccatga tgatcaagga ggtggccgaa gaaatcggcg tgcatccttc gacggtaagc
      661 cgtgccgtgg ctaacaaata tgcgcacacg ccgcagggcg tgttcgaact gcggtacttc
      721 ttcagcgaga gcgtgcaggg gccggagggc ggcaatacat cgctgctcat cctgaagcgc
      781 cgcgtcaaga agctcattga tgaggaggac ccgagccgtc cgttgaccga cgagcagatc
      841 acccggatcc tgcagtcgca gggaatccag gtgacgcgcc gcacggtcgc caagtaccgg
      901 gaagatatgc gcatcccaag cacgcaccag agaagggtga agaaatagcg acgagttgcg
      961 agtcccgagt tgcgagcgca accatcagga gatggctcca tccgccagtt cgcaacccac
     1021 gactcaggac tcacgactag ttccaatgca gtgcccgatt tgactcgagc agagaattct
     1081 taggaggggg cgctcatgaa cgtggaatat accggaaggc actacgaagt aactcccacc
     1141 atccgcaagg aagtagaaac tggcctcaca aaaatccgca aaattctggg cgaccggttt
     1201 gataccaagg taattctcgc cgtcgagaag caccgccata aagccgaaat caccatcagc
     1261 ccgcgcaacg gcccactggt gggactggcg caggcgcgcg acatgatgat cgcggtcaac
     1321 gatgccctgg agcatctgga gaagcaggct gtgaagtaca aggcgcgctg ggagaccaag
     1381 aagcgcgccg cgcgcaagac cgaagaggtc aacaagtgga acggccactc cacgcaggcc
     1441 gaggtgcaca cggcggtcgg attgtcggag aagaccgctg ttccggtcat ggtgcacaag
     1501 tttcccgccg tggccaagac gaccgaagtg cacctggttc gatccgagga tgcggtggcc
     1561 atgcgtccta tgacgctgga ggaggcgatc aaggaagcgc acttccgcga ccgggatgtg
     1621 ttcgtgttcc gcgaccctaa gggcaaggtg atggtgctgc accgcaagcg cgacgggaag
     1681 atggagttga tcgaagcgcc gtagagcttt cggctatcag caaagaattt gggtgcccca
     1741 cttctcgctg gttttgcgag aagtggggtt tttgagttcc gggatctctg taaatccgcg
     1801 aggcccgcag cggctaaagc cgagaacttt gtttgaggtc cggtcggcac ggctgaagcc
     1861 gcgctttccc gtttcggctg tccagcagca cggtagctca attttctttg tgggcgtatg
     1921 atccttccgc cgttgcaatc cacttcaatt tggagactgc tatgaagaag ctctttgggc
     1981 tcgtggtact gctgttgtgc ctcgcccctg cgctgtttgc tcaggagcac tacacggaag
     2041 gtcccatctg gcgcgtggag ctgattcgcg tcaagccgac tcacatggac gaatacctca
     2101 ccagtctgcg gcaatcgacc aagccgctga tcgaggagga gaagcgccag ggcgtgatca
     2161 tggactacaa ggtgtttctc aaggagacta agaatagtcc ggaagattgg gacatttgcg
     2221 tggccatcga attcaagaac catgccgcac tggatggtct ggccgccaaa ggcgaaatgg
     2281 cgcgcgacaa gattctgggc gggaaacagg cggcgcagca attgggcgag aagcgcgccg
     2341 agatacgcga aatcatcagc tcggagctac tgcaggagat tttcctgaag taataatggc
     2401 tgccccactc tgcgcgggtg ccaaagggtg gggcagttcg cgttgcggtc gagcaggcgg
     2461 ccgggatctc ggtcacttgc tcttgacggt gatgcccgcg gatggcaccg gtgctccgca
     2521 tttgccgccg cgcagtccgt ctagaaacgc ttttgtctcg agcagcacca acttttctgt
     2581 cgatcccgcg aaaccgatgc cggcgaagtt catgtgtgtg gcgcggtcga tcacgcccag
     2641 ccacttgcat cctgtgggca tgctctcata ggggatggtt cgggttttcc agtcgccgtc
     2701 gagtgcctga tctcgcgtgc cggtcaggat cagcatcggt ttgcgaactc cgctccaggc
     2761 attttccggg aacatctttc cgggaccgtc cggcgacagg gcaacatagg cgttgaagcg
     2821 atccatgcct ttcacatcga gagtgttgct cgcgccgacc tcgaccatca cggtgcgcgc
     2881 tcccatggag tgcccgagta gcgcgacgaa cggcggtttg caagccgact ccgcccagct
     2941 cagcgcggcg gcgatgtcga gtagccgcgc tttgtaggca gcggggtcat tcaccagttg
     3001 ctgcaatccg cgttttatgc cgcgcgactg ccgcacgtcg gaacgcaggg cagcgccgcc
     3061 gctctcctgg tggcccatca cgatcgctcg ccatccgtcg tcctgcaggg ccttggccag
     3121 ataggagtag ccgttctcgc tgccgcccgc tccgggagag atcaccgcca acggcggaca
     3181 gcctgtggtc tgcggcgcat agacgcggag cggtgtttgc gcgccatccg ggcgggaagc
     3241 ttgaaccagg tcctgcgccc aggaaagatc cgccggcaga atgacggcga gcaacagaat
     3301 cgctacagtt tgccaggggc gtttcaagtc cgctcctttt gcaggatatc gatccatcat
     3361 caaggtactg ccggcgcttt cgattggtaa gtgaaacaca aggttcgccg agagccggcg
     3421 agtgggtgcg aggacgctgg tattgatacg ttttaatacg gggaattgcg tgctagaatg
     3481 cgcggcggga gattccttgc catgatccgt acccgccgta gttgtaccgt gcttttcacc
     3541 ctgatgtcgt ccctcgcctt gggacgaggg cggctcgcgc acgcgcagac tgcttccttg
     3601 ccgcccaaac tttccgtgca ttgggaagaa ctgaccgcag ctgacttccg cgaaggcatt
     3661 cgccgatcgc agggaacctg cctgctgcct tt
//
LOCUS       DASPUL010000385        16203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1990070, whole genome shotgun sequence.
ACCESSION   DASPUL010000385 DASPUL010000000
VERSION     DASPUL010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 16203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16203)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16203
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1990070"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="VEJ00_09345"
     CDS             <1..1193
                     /locus_tag="VEJ00_09345"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXX01405.1"
                     /translation="GGIAMVFANAGIPVLLKDADRSSLDRGLATIQKNYASSVKRGRF
                     TQQFVDERLKLIRPTPSYDDFSKVDMVVEAVFEGMELKKEVFAQLDRTCRKGAILASN
                     TSTLNIDEIASATSRPEMVLGTHFFSPANVMRLLELVRGKRTSREVIATCMQLSKKLG
                     KVGVLVGNCRGFVGNRMFHEYGRQAVFLVEEGASPEAVDKALSDFGMALGPLATGDLA
                     GLDVGWRIRKEYRHLEKPGVRQPIVNDRLCELGRYGQKTGAGWYRYDEKRRAIPDPEV
                     NQLVRKWALEAGIRQREISPQEIVDRCIYSLVNEGARILEEGIALRAVDIDIIYVNGY
                     GFPPYRGGPMWYADTVGLKTVWERISEFHREYGEPWEPAPVLTRLAAEGKTFAEFAGE
                     QLVA"
     gene            1222..2334
                     /locus_tag="VEJ00_09350"
     CDS             1222..2334
                     /locus_tag="VEJ00_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HXX01406.1"
                     /translation="MNRLRDPTHNFVSCMKGSLLNRDTAPVRPGEELNAAVLAEWLRG
                     KVDGVERGIVIEQFPSGHSNLTYLLRVDGREYVLRRGPLGPVAPKAHDMAREFRVLQM
                     VHPLFREAPAVFHLCEDPAPLGAVFFLMERRHGTIMRDDVPVEVAALQNYPQLVSEAF
                     IDCLVRLHAIDVSTTGLMALGKPEGFLERQVTGWADRWQRAKTDDMPKMDEVIGWLAD
                     SRPASPAPTLVHNDYKLDNVMLPFGSVDGIEAVLDWEMTTVGDPLADVGLTLCYWAWA
                     GSPDLSSRGIPALTTQPGWYTRDQFVQRYAEKTGRDLSNIRYYEVLGIFKLAVILQQI
                     YYRFRRGQTQDSRFRNFDERVRGLVELAFSLSGTRS"
     gene            2331..3059
                     /locus_tag="VEJ00_09355"
     CDS             2331..3059
                     /locus_tag="VEJ00_09355"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXX01407.1"
                     /translation="MSTVYLVRHGQAGTRDAYDSLSDLGKQQARLLGEHFLSQGVRFA
                     AAYAGALSRQQQTAEGIRQAYVQNSVAFPEIQVDSGWDEFDLARVYREIAPQLSAEDP
                     EFRREYSDMQEQVRISNGTHAARIHRKWLPCDTKVVDAWIRGRFAYGGETWHQFRERV
                     ASCRLKKRGTQQRENILVVTSATPVAIWMGLSLEISDERLMHLAGVLYNTSYSIVRLR
                     DGQLRLFTFNAIPHLAAAGLRTHR"
     gene            complement(3073..3534)
                     /locus_tag="VEJ00_09360"
     CDS             complement(3073..3534)
                     /locus_tag="VEJ00_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXX01408.1"
                     /translation="MPLVLETPRSLKEWVGRQIGVTEWFLVSQDRIQRFADATEDRQW
                     IHLDRARALHESPYGTTIAHGFLTLALLSPLIKEAIEIKSSVRMSVNYGLNRVRFPSP
                     VRADSKIRAHFTLQSLKDLSDAVEAVFDAKVEVQGSDKPCCVAEWVVRYYS"
     gene            complement(3588..4040)
                     /locus_tag="VEJ00_09365"
     CDS             complement(3588..4040)
                     /locus_tag="VEJ00_09365"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXX01409.1"
                     /translation="MTIEHARNFLARVPFNNLLGMRLRSVHRDGITIECALRDELRNS
                     AGAAHGGVAAAMADAAVGIAIQRHFGGKRRITTVELKVNYFRPVSEGSIRARSHLLRI
                     GSTLCIGRVDLTDGRGRAVGTAIVTYIFIDLAATVGKGHRAARRSRKK"
     gene            complement(4126..5349)
                     /locus_tag="VEJ00_09370"
     CDS             complement(4126..5349)
                     /locus_tag="VEJ00_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXX01410.1"
                     /translation="MDFSFSDKVKDLQRRLQIFMDEYIYPNEQRFLDEIERDRWSPTR
                     VIEELKPKARADGLWNLFLPDYKDGGGLTNLEYAPLCEIMGRSVMAPEVFNCSAPDTG
                     NMEVLARYGTEEQKERWLKPLLAGEFRSCFAMTEPAVASSDATNIEASIVRQGKEYVI
                     NGRKWWASGAGDPRCKIAIFMGKTDPSAPPHRQQSMVLVPMDAAGVKVRRLLTVFGYD
                     HAPHGHGEITFENVRVPVSNMLLGEGRGFEIAQGRLGPGRIHHCMRCIGVAERALETM
                     CKRVQARVAFGKPLAEQGTIRADIARSRMEIEQARLLTLKSAYMMDTVGNKAARAEIA
                     MIKVVAPNVALRVLDRAIQAHGGAGVSQDTFLASAWANVRTLRLADGPDEVHTEAIAK
                     LELRKWSPPLPSARP"
     gene            complement(5353..6207)
                     /locus_tag="VEJ00_09375"
     CDS             complement(5353..6207)
                     /locus_tag="VEJ00_09375"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004857.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXX01411.1"
                     /translation="MSRQETLYDVANRVATITLNRPNRLNAWTAVMEAEVRSAMEEAE
                     GDDNVRVIVLTGAGRGFCAGADMSLLSAVAERGAVGHSSSQSAGSPSAGAVPPDPGLR
                     DDVSPDFQKRYSYFPAIGKPVIAAINGPAVGLGLVIALYCDLRLASEAARFSTTFAKR
                     GLIAEYGIAWLLPRMVGLANTLDLLFSARTIDANEALRMGLVNQVFPQQAFLEKVQGY
                     AAELASTVSPRSLRVIKRQVYEAMLAPLSRAFDVSVKEMMASFRTEDFKEGVAHFVEK
                     RPPAFTGR"
     gene            complement(6648..9542)
                     /locus_tag="VEJ00_09380"
     CDS             complement(6648..9542)
                     /locus_tag="VEJ00_09380"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXX01412.1"
                     /translation="MIGQTISHYRILEKLAGGGMGVVYKAEDVTLHRLVALKFLPENV
                     ANDSQALARFQREAQAASALNHPNICTIYEIGQQEGQPFIVMEFLDGVTLKYRIAGRP
                     LDTDHLLDLAIEIADALDSAHSQGIIHRDIKPANIFITKRGHAKILDFGLAKVKSAAE
                     SASGIAAAPTEGLTVEESHLTSPGSTLGTVAYMSPEQARAKDMDARSDLFSFGAVLYE
                     MATGKLPFRGESSAVIFKAILDATPIAPVRLNPEVPPDLERIISKALEKDRNLRYQSA
                     AEMRADLQRLKRDTESHVTAAVSAVDQPEGAIARRWLGKPYLYAAAGLLLGALAIAAV
                     YYRAHLNPRLSSKDTIVLADFSNNTGDPVFDDTLKTGLSVALNQSPFLNVLSDNAVAD
                     TLQQMTRAPGTRLTPELAREVCLRAGSKAYIAGSIATLGEEYVLGLKAINCRDGETLA
                     QEQVTSPSKERVLEVLGAAVSKLRTGLGESLATVQKFDIPLEHATTSSLEALKAYTLG
                     QKADREIGPGAALPYHQHAIQLDPSFAMGYMAVGGEYYSLSQTGRAAEYLTRAFELRD
                     HASEREKLAITATYYLNVTGELGKAAQAYQEEIESYPRNGGAHLNLGVVYAEQGQYDK
                     SVQMSLDELQRDPNNVAPYDNLADSYIALQRFDDARKTIHDAQTRKLDDYITHMAQYT
                     LAFYSSDSAGMAEQLQWFNGKPEENYGLALAADTEAYHGRLNSARGLVKRAVESAIRS
                     DSKENAGVWLENMALYEAGIGNRDEARQKAAEGLKLAPASQGVNSEAALAFAMAGETA
                     RAESLAKELNSQHPLDTQVQSLWLPGVRAELNLESGKPQAAVDDLQAASATELGQFNF
                     INNLSCLYDIYVRGQAYLVAGNASASANEFQKILDHNGIVWNCWTGALAHLGVARANA
                     LQARTAHGADADAARVRALAAYKDFLALWKDADPNIPILKEAKAEYAKLQ"
     gene            10537..11886
                     /locus_tag="VEJ00_09385"
     CDS             10537..11886
                     /locus_tag="VEJ00_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HXX01413.1"
                     /translation="MAQAVVAQSTDGTPGLALTGSQAKAAQSVSDGELTQARPTANRL
                     HPEAVQIPPQNPPADEGKTASLKLDLSIPETQLPNQKTSISPHIEIQLGAGSQPEQLT
                     SSAFPVAQNDADQPSAVMSAEADTANTQPAPPAPVDSKLLHVLVDPSAGTTVAAQITA
                     ADATNKPEAGIPHAAVSAEPAGPALPRVSVNQIGRQSTDPALPPISPATNRPASVDLK
                     KAPDRPDSPAKPKDRDFDKASSANPANSPEAGEQTQTQKQEVLQPQSQSIEQEKTVPG
                     IAGAPPALKATSAKTDVTAPQVPGDIIDLDGDESIPAQLHSMVNTARLAQGIDQSELR
                     IGMQMRELGNIDIRTSMAAHAFSAQIFVEHGEVAKTLTNELPGLYARLADQQVPAGQI
                     QIHGSGLSASAGFDHSGRQRSEESHASTQDLRGFKPETKAAPILEAAAAGDRLDIRI"
     gene            11900..12286
                     /locus_tag="VEJ00_09390"
     CDS             11900..12286
                     /locus_tag="VEJ00_09390"
                     /inference="COORDINATES: protein motif:HMM:NF015896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HXX01414.1"
                     /translation="MRINPLMLNMLAANQLNPPKATTADSTSTGSSGATGSTSNSNSP
                     ANSLANDASNMFLTLLVTQLKTQDPTSPMDPTQMVGQMLSMNQLNELIQIQQTLQAMA
                     GSPQSAASQSTQSAAASQYTQSQSIQ"
     gene            12299..13540
                     /locus_tag="VEJ00_09395"
     CDS             12299..13540
                     /locus_tag="VEJ00_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HXX01415.1"
                     /translation="MPNFSIPLSGLAAAQEELSVISNNLANLNTVGYKADQGSFKDLF
                     YQTYGMDGANNPVQVGEGVAIDSVTSNWTNGSIQATGVPTDAAITGNGFFVTQQNGVQ
                     EYTRDGSFTINSSGQLTTQDGQLVMGYPAQNGVISPSQTLSPLVINQGQLVPGAATTT
                     MQTQTNLDASAAVGDTFSTPIGVYDSLGTSHVLTLQFTKTASNTWGYQITLPAADTGG
                     TGNPTVVASGTLNFNGNGTLASPTAPVTGIAINGLADGASNMNLTWDLLDVNGNSLLT
                     QTTSPSATANATQNGFSSGTLVSFNIASDGTIEGSFSNSQTLALGQIALANFANPDGL
                     IRAGGNSFMTTLAAGSPVVGVAGTGGRGTITGGSLEQSNVDIATEFSNMIVTQRGYEA
                     NARLVTTFDQITQDTINMKQL"
     gene            13818..14111
                     /locus_tag="VEJ00_09400"
     CDS             13818..14111
                     /locus_tag="VEJ00_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor C-terminal
                     domain-containing protein"
                     /protein_id="HXX01416.1"
                     /translation="MATAQVAPVKGETSVADKAAAKTVFESFYWLPCQANVEIPVPGF
                     TVGDLLKLAVGEVVHTVTPSSKEVPIRINKIPLGSGKFEVVDERLAVRITEFA"
     gene            14108..14458
                     /locus_tag="VEJ00_09405"
     CDS             14108..14458
                     /locus_tag="VEJ00_09405"
                     /inference="COORDINATES: protein motif:HMM:NF016250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HXX01417.1"
                     /translation="MNDLSQLSTLAARVSTLPPEAGVTTRGSSLPPSNMLRSISRAAS
                     WILDKARKQQVRKVLKVCESVSLGEKRFVAVVQVEHERFLIGGAPTSVSMLARLEPES
                     FSEALRNSRQAGES"
     gene            14455..15237
                     /gene="fliP"
                     /locus_tag="VEJ00_09410"
     CDS             14455..15237
                     /gene="fliP"
                     /locus_tag="VEJ00_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01103.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HXX01418.1"
                     /translation="MKDILSRGLKMAAVSVLLCCSVLAVAATGAKPTHPAAAPSNEIR
                     ISLPGAPNSGSLSWTIVVLLTFLTLIPSLLLCMTPFARLLIVFHFLRQALGLQTTPTN
                     QTLIGLALVLTFFLMQPVGASILDQAVVPLEQGKLSAMDALQRGSMPVRTFMLHYVRE
                     KDVALFIDLAKEPRPRNPEDLSMRVVVPAYILSELKAGFQIGAVLFLPFMVIDMVVAS
                     ITTSVGMLQLPPVVISTPLKLLLFVMVDGWDLLVGSLMRSFS"
     gene            15237..15506
                     /locus_tag="VEJ00_09415"
     CDS             15237..15506
                     /locus_tag="VEJ00_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013480.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HXX01419.1"
                     /translation="MPAAQVIDVCRHTLLEVLLLAAPILLIAVAVSLLVNIVQVLTSL
                     QEVTISAVPRLLAVAAGTFFMMPWMWRQICQYTVSLLSDFHPYVR"
     gene            15509..>16203
                     /locus_tag="VEJ00_09420"
     CDS             15509..>16203
                     /locus_tag="VEJ00_09420"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HXX01420.1"
                     /translation="MDISITELLSGPLIVGLRVSGLMLFAPFFGNVAIPPRVKAVMVI
                     VITAVLYPVYGTKVGIVTLEQWPSVVAGETVIGVAIGLATNFVFESAQTAGSILGLQM
                     GYSLINVLDPQTQVDTPVVGVFHQMAAFLLFLQLDVHHWILRAIANSFDYLAPGSLHW
                     GSGLAGNLLHGVAGVLQTGLGIAAPVLAATLVADVLLGFLGKASPQMPLLLLGPALKS
                     LLGLALMVAAIRY"
BASE COUNT         3280 a         4785 c         4703 g         3435 t
ORIGIN      
        1 gtgggggaat cgccatggta ttcgccaacg ccgggattcc cgtgcttctg aaggatgctg
       61 accgatcttc gctcgaccgc ggactggcca caatccagaa gaactacgcc agttcggtca
      121 agcgcgggcg cttcacccag cagtttgtgg acgagcgcct gaaattgatt cggcccacac
      181 ccagctacga cgatttcagc aaggtcgata tggtcgtcga ggcggtcttc gaggggatgg
      241 aactgaagaa ggaagtgttc gctcaactcg atcggacgtg caggaagggc gccattctgg
      301 ccagcaacac gtcaaccctc aacatcgatg agattgcctc agcgacgtcg cggccagaga
      361 tggtcctcgg cactcatttt ttcagccctg ccaacgtgat gcgcctgctc gagctcgttc
      421 gcggcaagag aaccagcagg gaagtgatcg ccacttgcat gcagctctcg aaaaaacttg
      481 ggaaggtcgg tgtgctggtc ggcaattgtc gcgggtttgt cggcaatcgc atgtttcatg
      541 aatacggacg ccaggcggta tttctggtgg aagagggcgc cagcccggaa gctgttgaca
      601 aggccctttc cgacttcggc atggccttag ggccgctcgc aaccggcgat ttggcgggcc
      661 tcgacgtcgg ctggcgtatc cgaaaggagt accgtcacct ggaaaagccc ggcgtgcggc
      721 agccgattgt caacgaccgc ctttgcgaac tcggacgcta cggccaaaaa accggcgctg
      781 gctggtacag gtacgatgaa aagcggcgcg ccattccgga tcctgaggtg aaccaactgg
      841 tgcgcaagtg ggcactcgag gccggtatcc ggcagcgcga aatctcgcca caggagatcg
      901 tcgatcgctg catctactct ctggtgaatg aaggagcacg catcctcgag gaaggcatcg
      961 ccctgcgagc ggtagacatc gacatcattt atgtaaacgg ctacggattt ccgccgtatc
     1021 gtggaggtcc aatgtggtat gccgataccg tgggcctgaa aacggtttgg gagcgaattt
     1081 cagaattcca ccgtgaatac ggtgagccgt gggagccggc tccggtcttg acacgcctcg
     1141 cagccgaagg caagactttt gcggaatttg ccggggagca gctagtggca taagagcgct
     1201 gagaatcctg aggcctttgc ggtgaaccgc ctgcgggatc ctacgcacaa cttcgtttct
     1261 tgcatgaaag gctcactcct gaaccgcgat accgctcctg ttcgtccggg ggaagaacta
     1321 aacgcagccg tccttgccga gtggctgcgc gggaaagtgg atggagtcga gcggggcatc
     1381 gtcattgagc agtttccgag tgggcattcc aacctgacat atcttctacg cgtggacggc
     1441 cgtgagtatg tgctgaggcg cggcccgctg ggtccggtgg ctccgaaagc tcacgacatg
     1501 gcgcgtgaat tccgtgttct ccagatggtg caccctcttt ttcgggaagc tcccgcggtt
     1561 tttcatttgt gcgaagatcc agcgccgctg ggcgccgttt tctttctcat ggagcgccgg
     1621 cacggtacga tcatgcggga cgacgtcccg gttgaggtgg ccgctcttca aaattaccca
     1681 caactcgtca gtgaggcctt catcgattgc ctggttcggc tgcatgccat cgacgtttcg
     1741 acgaccggtt tgatggctct gggcaaacca gagggcttcc tcgagcgcca agtgacgggg
     1801 tgggccgatc gctggcagcg ggcgaagacc gacgacatgc ctaagatgga tgaagtgatt
     1861 ggatggctcg cagacagtcg cccggcctcg cctgcgccca cactggtcca caacgattac
     1921 aaactggaca acgtgatgct ccccttcggt tctgtcgacg gcatcgaagc ggtactcgat
     1981 tgggagatga ctacggtcgg cgatcctctg gccgacgtcg gcttgactct ttgctattgg
     2041 gcatgggccg gtagtccgga cttgagttcg cggggaatac cggcgctcac cacccaaccc
     2101 gggtggtata cccgcgatca gtttgtgcag cgctacgccg aaaagaccgg tcgcgatctc
     2161 tcgaacattc gttactacga ggtgctgggt atcttcaaac tggcggtgat tcttcagcag
     2221 atctactacc gctttcggcg cgggcagacg caggactcgc gattccgtaa tttcgatgag
     2281 cgagtacggg gcctggtcga gctggcattc tctctttcag gaacacggag ttgagcaccg
     2341 tctatcttgt tcgccacggc caggcgggca cgcgcgatgc ctatgactct ctctcggacc
     2401 tggggaagca acaggcgcga ttgctgggcg agcacttttt atcccagggt gtccgatttg
     2461 cagcggcgta tgcgggggca ttgtcgcgac agcagcagac cgcggaagga ataagacaag
     2521 cctatgtcca gaacagcgta gccttccctg aaattcaggt tgattccggg tgggacgaat
     2581 tcgatcttgc acgcgtatat cgcgagattg ctccacaact aagtgccgaa gaccccgaat
     2641 tccggcgtga gtactcagac atgcaggagc aggttcgcat cagcaacggt acgcatgcag
     2701 caagaatcca ccgcaagtgg ctgccttgcg acacgaaggt tgtcgatgca tggattcgtg
     2761 gccgcttcgc ctatggtggt gaaacctggc accagtttcg cgagcgtgtg gcatcgtgcc
     2821 ggttgaagaa acgtggcacg cagcaacgag agaatattct tgttgttacg tccgcgactc
     2881 ccgttgccat ctggatggga ctgtcgctgg aaatctccga cgagcgcctg atgcatctgg
     2941 ctggcgtctt gtataacacg tcatactcga tcgtgcggtt gcgggacggc cagctgcggc
     3001 tgtttacctt caatgcgatt ccgcacctcg cggctgcggg gctgcgaacc catcgctgaa
     3061 tggctgggca ttttaggagt agtagcgaac cacccactcg gccacgcagc acggcttgtc
     3121 gctgccctgg acttccacct tcgcgtcaaa taccgcttcc acggcatccg acaggtcctt
     3181 caatgattgc agagtaaagt gggcccgaat cttcgagtcc gcccgtacgg gcgatgggaa
     3241 gcgaacccga ttcaaaccgt agttcacact catgcgaaca ctgctcttga tctcgatcgc
     3301 ctctttgatc aaagggctga ggagcgcgag agtcaggaag ccgtgggcaa tagtggttcc
     3361 gtagggagat tcgtgcagcg cgcgtgcgcg atccagatgg atccactgcc ggtcttccgt
     3421 tgcgtccgca aagcgttgaa tgcgatcctg agacaccagg aaccactcgg ttactcctat
     3481 ttggcggcct acccactctt ttagagagcg aggtgtttcg agcacaagtg gcactcatat
     3541 ctcctgcgac agttttcttt atggcgcggt tgcattgacc cggcttacta cttcttgcgt
     3601 gaacgacgtg cagcgcgatg acctttccca acagtcgcgg ccaaatcaat gaatatgtaa
     3661 gtgacgatgg ccgtcccgac ggcccgtcca cgaccgtcgg tgagatccac tctgccaatg
     3721 cacaaagtcg agccgattcg cagaaggtgc gaacgcgccc ggatggagcc ttctgacacc
     3781 ggtcgaaaat agttgacttt caactcgacg gtggtgatcc gccgcttgcc tccgaagtgg
     3841 cgctggatcg cgatgccaac ggcggcgtct gccatcgctg ctgctacgcc gccgtgggct
     3901 gctccggcgc tattgcgcaa ctcatcgcga agcgcgcact cgatcgtaat gccgtcacga
     3961 tgcacgcttc ggagccgcat cccgagcagg ttgttgaacg gcactcgcgc caaaaaattg
     4021 cgggcgtgct caatggtcat agccctcact taaaccgcat cacttgctcg cttccagatc
     4081 ctcacgcact gtgggcgtgg aatgactaga gattctgaaa agcattcagg gacgcgcgga
     4141 tgggagcggc gggctccact tccgaagctc cagtttcgca atcgcttcgg tatggacctc
     4201 gtcgggaccg tccgccagcc gcagcgtgcg cacattcgcc cacgcgctgg ccagaaatgt
     4261 atcctgcgac acgcccgcgc ctccgtgcgc ctggatcgcg cggtccagca cgcgcaaagc
     4321 gacattcggg gccacgacct tgatcattgc gatctcggcg cgtgcggctt tgtttccgac
     4381 ggtatccatc atgtaggcgg acttcaaggt cagcaggcgg gcctgctcga tctccatgcg
     4441 ggagcgggcg atgtcggcac gaatcgtgcc ctgctcggcg agcggcttgc cgaacgccac
     4501 gcgggcctgc acacgcttgc acattgtttc gagcgcccgc tcggcgacgc cgatgcaccg
     4561 catgcagtgg tgaatgcgcc caggtcccag cctgccttgc gcgatctcaa aaccgcgtcc
     4621 ttcccccagc agcatgttcg agacgggtac gcggacattt tcgaatgtga tctcgccgtg
     4681 gccgtgcggc gcgtggtcat agccgaacac cgttagcagt cgcctgacct tgactcctgc
     4741 ggcatccatg ggcaccagca ccatcgactg ctgtcgatgc gggggagcag acgggtccgt
     4801 cttgcccata aagatcgcga tcttgcagcg cgggtcgccg gcgcccgagg cccaccactt
     4861 gcggccattg atgacgtatt ccttcccttg gcgaacgatg ctcgcttcga tgttggttgc
     4921 gtcggaggag gccacggccg gttcggtcat cgcgaaacat gaccggaact cgccggcgag
     4981 caagggtttc agccaacgct ccttttgttc ttctgtgccg tagcgggcga gcacttccat
     5041 gtttccggta tcgggcgccg agcagttgaa tacctcggga gccatgacgc ttcgccccat
     5101 gatttcgcac agcggggcat actcgagatt cgtcagcccg ccgccgtctt tgtaatccgg
     5161 caagaacagg ttccacaggc catccgcgcg cgccttgggc ttgagttcct cgatcacgcg
     5221 cgtcggcgac cagcggtcgc gctcgatctc atcgaggaag cgctgctcgt tcggatagat
     5281 gtactcgtcc atgaagatct gcaatcgccg ttgcagatcc ttcaccttgt cgctgaacga
     5341 gaaatccatg cgttatcttc cagtgaaagc cgggggccgc ttctccacga aatgggcgac
     5401 gccctctttg aaatcctcag tccgaaaact cgccatcatc tctttcacag agacatcaaa
     5461 cgcccgggag agcggcgcca gcattgcctc ataaacctgg cgcttgatca cacgcagtga
     5521 gcgcgggctc acggtggacg caagctcggc agcgtatccc tgcactttct cgagaaaagc
     5581 ctgctggggg aagacctggt tcaccaaccc catgcggagt gcctcattgg catcgattgt
     5641 ccgggcagag aacaacaggt cgagagtgtt ggccaggccc accatccggg gcagcagcca
     5701 cgcgataccg tattcggcga tgagtcctcg ttttgcaaag gtggtgctga acctggcagc
     5761 ttccgaggcg agccgcaagt cgcaataaag cgcgatcacc aggcccaggc caaccgcggg
     5821 gccattgatg gcagcaatca ccggtttccc gatcgccgga aagtaggagt accttttttg
     5881 aaagtcaggc gagacatcat cgcggagacc ggggtcaggt gggaccgcgc ctgcgctggg
     5941 actgcccgca ctttgactgc tcgaatgacc gaccgccccg cgctccgcca ccgcgctcag
     6001 gagcgacatg tcggccccgg cgcagaagcc ccgtccagca ccagtcaaga cgatgactcg
     6061 aacattgtcg tccccttcgg cttcttccat ggccgagcgc acttctgctt ccatcaccgc
     6121 ggtccaggcg ttgagccggt tcggccggtt gagggtaatc gtggccacgc gattggcgac
     6181 gtcgtagagg gtttcctgcc tggacatgag gcgcgattat ataaaaatca ctggcgttag
     6241 tcactctcat gttagagcga ctatcgcaat gtgggagacc gggggtgaat cgcgcaaccg
     6301 ggagtttacg tttcgttgtc gctctgagct tctggctggt cggcagacat accatgccgg
     6361 gctgcccagc acagtgcggg cgcacttgac ctgttattgc ttcgttttcg gaaccctgcg
     6421 ttcatcttcg ctcgactctt cgccagatgg ctttccgatc tcatcgacat cgacagtttc
     6481 ggaacattcc ccctggtcgt catagtcgca tgcccgaccc gggtgggcct gtttgcactt
     6541 ctggcaaaat tctgtagcca tttcccgttc cccccatttt ggccaaatcg atcgtcaagc
     6601 tgtgtaacac tgtggcatcc acggaggtgg gccgtcaata tccgcattta ttgcagcttc
     6661 gcgtactcgg cttttgcttc cttcaggatg gggatgttgg gatcggcatc cttccaaaga
     6721 gcaagaaaat ctttgtacgc cgcgagggca cgaacgcggg cggcatcggc atccgcgccg
     6781 tgcgctgtcc gggcctggag tgcattggcg cgggccacgc ccagatgcgc cagggctcct
     6841 gtccagcaat tccaaacgat gccgttgtgg tccaaaattt tctgaaactc gttcgccgat
     6901 gcactcgcat tgcctgcgac gaggtatgcc tggccgcgaa cataaatgtc gtagaggcaa
     6961 gacaggttgt taatgaaatt gaattgtccc aactcagtgg cagaggccgc ttgcaggtca
     7021 tcgacggcgg cctgtggttt gcctgattcc agattcaact cagcccgcac tccaggcagc
     7081 caaagagact gcacttgagt gtccagcgga tgctgcgaat tcagttcctt tgccaaggac
     7141 tcggcacgcg cggtttcgcc cgccatggca aatgccaacg cagcctcgct gttcacgccc
     7201 tggcttgccg gagccagttt cagcccttcc gctgcttttt ggcgtgcttc gtcgcgattg
     7261 ccgatgccag cttcatagag ggccatattt tccagccaca ctccggcatt ctccttgcta
     7321 tcggagcgaa tggcagattc caccgcccgc tttaccagtc cccgcgctga gttcaggcgt
     7381 ccgtggtagg cctcggtgtc ggcagcgagc gccagcccgt agttttcctc gggcttgccg
     7441 ttgaaccact gcagttgttc cgccatgccg gcagagtccg aggagtagaa cgcgagcgtg
     7501 tactgggcca tgtgcgtgat gtagtcatcc agctttctcg tctgggcgtc gtgaatggtt
     7561 ttgcgagcgt catcaaaccg ttgcagcgcg atgtagctgt cggcgaggtt gtcatacggg
     7621 gctacgttgt tgggatcccg ctgaagttcg tccagcgaca tctgtaccga cttgtcgtac
     7681 tgcccctgct cggcatatac gacgccgagg ttaagatgag cgcccccgtt tcgggggtag
     7741 ctctcaatct cctcctgata ggcctgcgcc gccttgccga gttctccggt gacgttcaga
     7801 taatacgtgg ccgtgatcgc cagcttctcg cgttcgctgg catgatcgcg taactcgaag
     7861 gcccgggtga ggtattccgc ggcgcgccct gtctgggaga ggctgtagta ctcgccgcct
     7921 acggccatat agcccatggc gaagctggga tcgagctgaa tggcgtgctg gtgatagggt
     7981 agagccgcgc ctggaccgat ctcgcgatca gccttctgac caagagtgta cgccttcaag
     8041 gcttccagcg atgacgtcgt agcatgctcc agggggatgt caaacttctg caccgtggcc
     8101 agtgattccc ccagtccggt gcgcagtttg gagaccgcag cgcccagaac ttccaggacc
     8161 cgctccttgg agggagacgt aacctgttcc tgggccagcg tctcgccgtc gcgacagttg
     8221 atcgccttca gtcccaggac gtattcctcc cccaaggtgg cgatggagcc ggcgatgtag
     8281 gccttgctgc ccgcccgcag acaaacctcg cgtgcgagtt cgggggtgag tctggtgccg
     8341 ggggctcgcg tcatttgctg cagtgtgtct gccaccgcgt tgtcagagag cacattcagg
     8401 aagggcgact ggttcaaggc gacgctcagc ccggtcttga gtgtgtcatc gaaaaccggg
     8461 tcacccgtgt tgttcgaaaa atcggcaagc acaatggtgt ccttgctgct gaggcgtgga
     8521 ttaagatgtg cacggtaata aacggcggca attgccagag cacccagcaa caaaccagcc
     8581 gccgcataca aatacggctt tcccagccat cgacgagcga tggctccctc cggctgatca
     8641 actgcagaaa ccgcggctgt tacgtgcgac tcggtgtcgc gcttcagacg ctgcaggtcc
     8701 gcacgcatct ccgcagcgct ctgataacgc aggttgcggt ccttctccag cgctttgctg
     8761 ataatccttt ccagatctgg cggtacttcc ggattcagcc tcacgggcgc aatcggcgtc
     8821 gcatcgagaa tggctttgaa gatcaccgca gaactctcgc cgcgaaacgg gagcttgccc
     8881 gtcgccatct cgtaaagcac ggcaccgaag gagaacaaat cgctgcgcgc atccatatct
     8941 ttcgctcgcg cctgctcggg agacatatac gccacggttc ccaaagtcga gccggggctg
     9001 gtgagatggg actcttccac cgtcaagcct tcggtgggtg cggcggcaat tccgctcgcc
     9061 gactccgccg ctgacttcac cttggcaagc ccaaaatcga gaatcttggc gtgtccacgc
     9121 ttggtgatga agatgttcgc gggcttgatg tcgcgatgaa tgatgccctg ggagtgcgca
     9181 gagtcgagtg catcggcaat ctcgatcgcc agatccagca agtggtccgt atcgagcggt
     9241 ctacccgcga tgcgatactt caacgtcacg ccgtcgagaa actccatcac gataaacggc
     9301 tgaccttctt gctggcctat ttcgtaaatc gtgcagatat tggggtggtt cagtgcggaa
     9361 gcagcctgtg cttcgcgctg aaagcgggcc agggcctggg aatcgttggc cacgttctcg
     9421 ggcaggaact tcagagcgac gagccggtgc aacgtcacat cctcggcctt gtagaccaca
     9481 cccatcccgc cacccgcgag cttctcgaga atgcggtagt gcgaaatggt ctgaccgatc
     9541 atttgcggag aaatggtagg gccgcagata gagcaagtca atgcgcgggg gttatatgcg
     9601 ggtaaagagg gggcttcgct gagcgcgcga aggcacctgc cgaattcgtt ttgaggggca
     9661 gagtcggcag tttttgccca gagccaaata ggcaatcctt gcctatccga cgctatcggg
     9721 ctggaatttg gcagttgtat atcggcatct tcttcccaag tctttagtgc ggagcgagtt
     9781 ttcccatagc aaaaaaattc ccgaatcggg cggctttggt gcaccgcatg cattgtcgac
     9841 gaccgactca ttgtgaatgc aaccatacaa gacgggaatg ccagcctggc gtcgggaaag
     9901 agtgcgctcc cgcatgcgtt ggacctctcg gcggggttcc tgccggcgaa agcgtcagaa
     9961 gccggtccgg gcagcgaatt cagcgcggcg gtcgcatcct tctttgccga gccccccaac
    10021 gcccctttgc cgaagtcgag cccgtcccag attgttcatg cagcacagaa gtcggcgcct
    10081 cgcagtgttc ccaacgcgga tacgaaagag atacaggatc cgcccggacg aagtatgaac
    10141 acggtgacag cctctgtgcc agtggtgccg ccgccctcga ctgcatttgt accggagctc
    10201 cccaaaccgc ccaaagccgg ggattcgcgc gtcgacgagt cagcaaagac agcagatctg
    10261 cccagtgcga aactcgggag tgccgaggtt ccgcccaagg gccctcatcc aaatgtcgat
    10321 gcggaggtgg ggatcagcca gttctcgcat tcgctggatg tgcagaatga ctccggcccc
    10381 gacgctcgcc ggattcagtt agctgagatt ccgtcgcagg agccgctgcc acagtctcgg
    10441 gtctcctcaa cgcaaacggc cgtgtctcga ttcgaagtac aactgcccga aatcgcatca
    10501 ccccccgcga caaccgatcc gcgcgctgat ctgcaagtgg cccaagcagt ggttgcccaa
    10561 tcgaccgacg gcacgccagg actcgcgctt accgggagtc aggcaaaagc agctcagagc
    10621 gtcagtgacg gtgaactcac gcaggctcgg ccgacagcga atcgcttgca tcctgaggct
    10681 gtccaaattc cgcctcagaa tcctccagct gatgaaggga aaacggcctc tctaaagttg
    10741 gatctgtcca ttcccgagac acaactgccc aaccaaaaga cctctatctc tccccatatt
    10801 gagatccagc tgggagcagg ttcgcaacct gagcagctga cgagctccgc atttccagtg
    10861 gcacagaatg atgcggacca accctctgcg gtcatgtcgg cggaggcaga taccgccaac
    10921 acgcagcctg caccccccgc gcccgtcgat tcgaagctct tgcatgtgct tgttgacccc
    10981 tccgcaggaa cgacagtcgc tgcacaaatt acagcagctg acgcgacaaa caagccggag
    11041 gccggaattc cccatgccgc ggtgagtgca gaaccagcgg gcccggctct accacgagta
    11101 tccgtaaatc agatcggcag gcagagcacg gaccctgcgt tgcctccaat ttcgccggcg
    11161 acaaatcgtc cagcaagcgt ggacctgaaa aaagcaccgg atcggcctga ctccccggcc
    11221 aagcccaagg atcgcgattt cgacaaagct agctcggcca atccagctaa cagcccggag
    11281 gcaggcgagc agacgcagac tcagaagcag gaagtgcttc aaccgcagag ccagtccatc
    11341 gagcaggaga agacggtacc cggtatcgcc ggtgcaccac cagctcttaa agccacttca
    11401 gcgaaaactg atgtcacagc tccgcaagtg cccggggaca tcattgacct ggacggtgat
    11461 gagagcattc ccgcgcagct gcattcgatg gtgaacacgg ccaggcttgc gcaggggatc
    11521 gatcagtctg aacttcgcat cggtatgcag atgcgggagt tgggcaacat tgacattcgc
    11581 acctccatgg ccgcgcacgc cttctccgct caaatcttcg tggagcacgg agaagtggcg
    11641 aagacgctga caaacgagtt gcccggcctg tatgcacgcc tggcggatca acaggtgcca
    11701 gcaggacaaa ttcagatcca cggctccggc ttgtccgcgt cggccggttt cgaccacagc
    11761 ggtcgacaac gatccgagga gagtcatgcc tcgactcagg acctgcgcgg atttaagccg
    11821 gagacgaaag ccgccccaat tttggaagca gcagcagccg gtgacaggct cgacattcga
    11881 atttaattga ggaggaagta tgaggatcaa tcccttgatg ttaaacatgc tggctgcgaa
    11941 ccagctcaat ccgccaaagg cgactacggc cgacagcacc agtacaggca gcagcggcgc
    12001 tactggcagc accagcaata gcaattcgcc cgctaattcc cttgcgaacg acgccagcaa
    12061 catgttcctg accctgttgg tgacacaact caaaactcag gatccgacct cgcccatgga
    12121 cccaacccag atggtcggcc agatgttgtc gatgaaccag ctcaacgagt tgattcagat
    12181 ccaacagacg ctgcaggcca tggccggttc gccgcagtcc gcggcctcgc aatcgaccca
    12241 gtccgcagca gcttcacagt acacgcaatc gcagtccatc caataggaga caacgacaat
    12301 gcccaatttt tcgattccac tttctggttt ggcagccgct caggaagagc tgtctgtcat
    12361 ttccaacaac ctggccaacc tgaatacggt tggctacaag gcagaccagg ggtcgtttaa
    12421 ggacctcttc taccagacgt acgggatgga cggcgcaaac aatccggtgc aggttggaga
    12481 aggcgtcgcc attgattctg taacgtccaa ctggacgaat ggaagcatac aggccacggg
    12541 agtgccgacc gatgccgcca ttactgggaa tggatttttt gttacccagc agaacggcgt
    12601 acaggaatac acgcgagacg gcagcttcac gatcaactca tcgggacagt tgacgactca
    12661 ggatggtcaa ctcgtgatgg gatatccagc ccagaacgga gtgatcagtc catcgcagac
    12721 actgtctcct cttgttatca accaaggtca actcgtcccc ggggcggcca ccacaaccat
    12781 gcaaacccag accaacctcg atgcgagtgc cgcggtcggc gacacttttt ctacccccat
    12841 cggcgtttat gattcgctgg gtacatcgca tgtgctgacg cttcaattta cgaagaccgc
    12901 ttcgaacacg tggggatacc aaatcacgtt acccgcggcg gacactggcg gaaccggcaa
    12961 cccaacggtc gtggcctccg gcactctgaa cttcaacggc aatggaacac tggccagccc
    13021 gaccgctcct gtgaccggaa ttgccatcaa cggattagct gatggcgcgt ccaatatgaa
    13081 tctgacctgg gacctgctcg acgtcaacgg aaattcactc ttaacccaga ccacgtctcc
    13141 cagcgctaca gccaacgcga cacaaaacgg cttcagcagt ggaactctgg tcagtttcaa
    13201 tatcgcctca gacgggacga tcgaaggttc cttcagcaac agccagacgc ttgcacttgg
    13261 ccagatcgct ctcgcgaatt ttgcaaaccc agacggcttg attcgcgccg gcggcaacag
    13321 cttcatgacg actctggccg caggttcgcc agttgtcggt gtggcgggga cgggtggtcg
    13381 cgggacgatc acgggcggct ctctcgagca gtccaatgtg gatattgcaa ccgaattttc
    13441 gaacatgatc gtgacccagc gtggttacga agccaacgcc cgtttggtca ccacgttcga
    13501 tcagatcacg caagacacga tcaacatgaa gcagttgtag gctgaatagg ttaacggacg
    13561 attagacact ttccggcgcg aactgcaatc gcgccggaaa gtggcagctc tactccggct
    13621 ctaatcgaga atggtcaatt ctgacagttc gtgtaccgcc gtaatcaccg cctgctaact
    13681 ccttttcttt tgtgctcttt cgaccaagga tcacaagcga tttgatcttt gggatttttc
    13741 tcgcctccgg cccagggatt gcacttctcc ggtttctctc tgggtgttgc tgtctgtccc
    13801 agagacttag agcgaagatg gcgacagccc aagttgcacc cgtaaaaggc gaaacatccg
    13861 ttgccgacaa agccgcagcg aagaccgtct ttgagtcttt ttattggctg ccctgtcagg
    13921 caaacgtgga aatcccggtc ccgggcttta ctgtgggcga tcttttgaaa ctggccgtag
    13981 gcgaagtcgt gcacactgtt accccgtctt ccaaagaagt acccatacgc atcaacaaaa
    14041 ttcctctggg ctcgggaaag ttcgaagttg ttgacgagcg ccttgcagtc cgcatcacgg
    14101 agtttgcatg aacgatctgt ctcaactgag cacgctggca gcccgagtca gcaccttacc
    14161 cccagaggct ggggtaacca cgcgaggaag cagcctgccg ccttcgaaca tgctgcggtc
    14221 catttcgcgc gcggcttcgt ggattctgga caaagcgaga aagcagcagg tgagaaaggt
    14281 cctaaaggtt tgcgagagcg ttagcctggg agagaagcgg tttgtcgcgg tcgtgcaggt
    14341 ggaacacgag cggtttttga ttggcggagc ccccacttcc gtctcgatgc tggcacggct
    14401 ggagccagaa agcttctccg aagctctgcg aaacagccgt caggcgggag aatcatgaaa
    14461 gacattctct cccgcggact gaagatggcc gcggtgtccg ttctgctttg ttgttcagtc
    14521 ctggcagtag cagcgactgg agcaaaaccc acgcacccgg ctgccgctcc ctcgaacgaa
    14581 attcgaatca gcctgcccgg cgcgccaaat tcaggctcac tctcctggac aatcgtcgtt
    14641 ctgctgacgt tcctcacgtt gattccatcg ctgctgctct gcatgacgcc atttgcgcgc
    14701 ctgctgatcg tctttcattt tctgcgacaa gcgctcggac tgcagaccac tcccacgaat
    14761 caaactctga ttggcttggc cctggtgctg acgttctttc tgatgcagcc ggttggagct
    14821 tccattcttg accaagccgt agtgccgttg gaacagggaa aactctcggc tatggacgcc
    14881 ttgcaacgtg gttccatgcc ggttcgcacg ttcatgctgc attacgttcg ggaaaaggat
    14941 gttgccttgt tcatcgatct tgccaaggaa cctcggccgc gcaaccccga ggatctctcg
    15001 atgcgggttg tggtccccgc atacatcctg tccgaactca aggcagggtt tcagatcgga
    15061 gccgtgttgt ttctgccctt catggtgatc gatatggtgg tggcgtcgat tacgacctct
    15121 gtcggcatgc ttcaactgcc tccggtcgtg atctcaactc cgctgaaact gctgttgttt
    15181 gtgatggtag atggctggga cctgctggtt gggtccctga tgaggagctt cagctaatgc
    15241 ccgcggctca ggtgatcgac gtttgccggc acacgctgct ggaagttttg ttgctggcgg
    15301 caccaatcct gctcatcgca gttgcggtca gtttgctcgt gaatattgtc caggtgttga
    15361 catcgttgca ggaagtgacc atctctgcag tgccgcgtct tctcgcggtc gcggccggca
    15421 ctttcttcat gatgccctgg atgtggcgac agatatgcca atacacagtt tcgttgttat
    15481 ctgattttca tccgtacgtg cgctagatat ggatatttct atcacggaat tgttgagcgg
    15541 cccgctgatc gtgggactgc gcgtctccgg attgatgttg ttcgcgccgt ttttcggcaa
    15601 cgtcgccatt cccccacgag tcaaggcagt gatggtaatt gtgatcacgg cggtcttgta
    15661 tccggtgtac ggaacgaaag tgggcatcgt cactctcgaa caatggcctt cagtggtggc
    15721 cggggaaaca gtgatcggtg ttgccatcgg tctggccacc aacttcgtct tcgaatccgc
    15781 gcagaccgca ggctcgatcc tgggccttca gatgggatat tcgctcatca acgtgctgga
    15841 tcctcagacc caggtggaca cgcccgtggt tggcgtcttt caccagatgg ccgccttttt
    15901 gctctttctg cagcttgatg tacatcattg gattcttcgc gctattgcca acagctttga
    15961 ctatctcgcg ccgggatcgc tgcactgggg aagcgggctc gccggaaacc tgctgcacgg
    16021 agttgctggc gtgctccaga ccggtctggg gattgcggcg cccgtgctgg ccgcgactct
    16081 ggtagccgat gtgctgctcg gatttttggg caaagcttct ccccagatgc ctttgctgct
    16141 gctggggcct gcgctgaaga gccttctggg tctggcgttg atggttgcgg cgattcgcta
    16201 ctg
//
LOCUS       DASPUL010000386         5876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1438100, whole genome shotgun sequence.
ACCESSION   DASPUL010000386 DASPUL010000000
VERSION     DASPUL010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438100"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VEJ00_09425"
     CDS             complement(<1..392)
                     /locus_tag="VEJ00_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXX01421.1"
                     /translation="MPVKPIPDGYHTLTPFLTVRDAARAVEFYKQAFGAEERGIAKGP
                     DGKVMHAEVKIGDSIIMLSDEFPDFGCLSPQSIGGSSSGLHIYLENVDAAFDRAVKAG
                     AKVEMPVMDQFWGDRYGKLQDPFGHKWS"
     gene            complement(602..1498)
                     /locus_tag="VEJ00_09430"
     CDS             complement(602..1498)
                     /locus_tag="VEJ00_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXX01422.1"
                     /translation="MIKQTLRNPALRKRATQFGRLVRHSAVTPRCGQTHKPRLVTNGE
                     LGLTFIGHASFFLQLGGHSVVIDPNFARWLFVLKRLRRPGLRIADLPAIDLVLVSHAH
                     FDHLHRPSLRALVQNSLRLSGTAPAIIVPTHVSDLVSDLGFSEIIELDWWKSSRHGNL
                     AVTHVPSRHWGARILKDSHRGYGGFVLKAGKHSVYHAGDTAYFGGFAEIGRRLAPELA
                     LLPIGAYNPPQFRNVHADPSDATRAFLDLKARWMVPMHYGTFRLSHEPVDEPLQLLDR
                     EARAAGIQDRVVVMEEGITRFF"
     gene            1795..2679
                     /locus_tag="VEJ00_09435"
     CDS             1795..2679
                     /locus_tag="VEJ00_09435"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXX01423.1"
                     /translation="MRVAFLGLGIMGQPMAANLVKAGHEVTVWNRTPGKLVEGAAVAP
                     TPAAAAQGAEVVWMCVSDTDAVENVLFGPDGAESSLAEGMIIADSSTISPSATLKFAE
                     RVRAKGVAWVDAPMTGSKIGARDGTLVFIVGGDEAVIERLKPLFAAMGKKIFRMGETS
                     KGQATKLAMNLQIAMIFEGFAEALTLATKLGVDAQQLMSLIDATMVRSGVVDYKAPFI
                     LQRDFTPNFPLRLMHKDIRLTLEAAKEARVKLPGLETVEEIYEMATEDGHKDLDYAAT
                     LTLLEKWAGVQVKGKAAA"
     gene            2823..3740
                     /locus_tag="VEJ00_09440"
     CDS             2823..3740
                     /locus_tag="VEJ00_09440"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXX01424.1"
                     /translation="MELQDLEKVQTPKDEINWITAIFMALFHVGAVAALFFFTWKALL
                     VAVFLWWVAGSLGIGMGYHRLLTHRGFKTYKWVEYFLTFCATLALEGGPIFWVATHRI
                     HHQYSDQDGDPHSPIDGKWWAHMGWILTGKSMHHDATTLARYVPDLAKDKFHVWMTKY
                     HYVPQIILGIALFAIGGLPWLLWGTFFRTVFLLHCTWLVNSATHSWGSRRFATRDLST
                     NSWWVALLTFGEGWHNNHHAHPTSAAHGFTWYEIDTNWYGIWLLKTFGLAWDIKRVSL
                     ADIERGLVAAANGRLIAPSNAAEFWAAGD"
     gene            3890..4603
                     /locus_tag="VEJ00_09445"
     CDS             3890..4603
                     /locus_tag="VEJ00_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01425.1"
                     /translation="MFGGPNRVPERISSHVASNLDPSHERFFLYVPSSYTGTESYGMI
                     VFTYADPEARLPYGWQQVLDSRKYIFVAAQNAGNDQPRGRRLGLAVMGALKTMKAYNI
                     DRNRVYASGFSGGARMAGLLAFYQSDIFHGTIQNCGADFYRQVPTVNAVSQVDTAGQA
                     YGVLEASQDEIQRAKTLRFVLITGTNDFRRGNILDIFHGGFERDGFHAKLFDMAGMGH
                     DICDSETLTHALDFLESGS"
     gene            complement(4759..>5876)
                     /locus_tag="VEJ00_09450"
     CDS             complement(4759..>5876)
                     /locus_tag="VEJ00_09450"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXX01426.1"
                     /translation="IRDSVIPAYVKFTAFVRDEYAPKGRSEPGVWALPDGDARYAFRV
                     KEVTTTNLTPEEIHQIGLAQVKAIEERMSGVARQLGFPDLKSFYGSIKANPALHPQSR
                     QQILDLYQKYIDQMYTKLPELFGRLPKEHLDVKPIEEYREKEASTHYLEGTPDGSRKA
                     HVMVATGDFQNRTTLDIETTAYHEGVPGHHLQISIAQELPELPPFRQHEFYTAYTEGW
                     ALYSEELGKEVGFFQDPYSYYGHLQDDMLRAIRLVVDTGFHYKHWSRQQVVDFFHEHS
                     AIDEPEVQEETDRYMANPAQALGYKVGQLEILKLRQYSKDQLGEKFDVRAFHDEVLSG
                     GALPLDVLGNRIHGWVAQQKAVTSAQSASPVQTMQRK"
BASE COUNT         1150 a         1635 c         1871 g         1220 t
ORIGIN      
        1 atggaccatt tgtggccgaa cgggtcctgc agcttgccat agcgatcgcc ccagaactgg
       61 tccatgaccg gcatttccac tttcgctcct gccttgacgg cgcggtcgaa ggcggcatcg
      121 acattttcga gataaatgtg cagaccggac gatgatccgc cgatcgactg cggcgacagg
      181 cacccgaagt cggggaattc atcgctcagc atgatgatgg agtcgccgat cttcacttcg
      241 gcgtgcatga ccttgccgtc tggccccttg gcaatgccgc gctcttcggc gccgaaggcc
      301 tgcttgtaga actcgacggc ccgggcagcg tcgcggaccg tgaggaaggg tgtgagggta
      361 tggtatccgt cgggaatggg cttcacgggc atgatttcct cctgggacga agatttggcg
      421 aaaggagtat acatcaaacg agccggttgt ggacgtaaag cctgaaggcc gtcgaggttt
      481 ctctgcccgc tctccgaggt ctgtgcgtca agatctctta tcgcgaagag cgcggagaac
      541 cgctgtagtg ggcgcagaga acaaatcttg ggtcaggaat gggacgggca acgcggttcc
      601 gtcagaagaa ccttgtgatg ccttcttcca tcaccaccac gcggtcctga atgccggcgg
      661 cacgggcttc gcggtcgagc aactgcagcg gctcgtcgac cggctcgtgc gacaggcgga
      721 aagtgccgta atgcatgggg accatccagc gcgccttgag atcgaggaag gctcgggtcg
      781 cgtcggaggg gtcggcgtgg acgttgcgga actgcggcgg gttgtaggcg ccgatgggga
      841 gcagagcgag ctcgggagcc aggcgccgtc cgatctcggc gaagccgccg aagtaggcgg
      901 tgtcgccggc gtgatagacc gagtgcttgc cggctttcag cacgaagccg ccgtagccgc
      961 gatgcgaatc cttgagaatg cgcgcgcccc agtggcgcga gggcacgtgc gtcacggcca
     1021 ggttgccgtg gcgcgagctc ttccaccaat cgagttcgat gatctcggag aagccaaggt
     1081 cggaaaccag gtccgacaca tgagtcggca cgatgatcgc cggtgcagtt ccgctcaggc
     1141 gcaggctatt ctgtaccaag gcccgcagcg acgggcggtg caagtgatcg aaatgcgcgt
     1201 gcgacaccag caccagatcg atggccggca gatcggcaat gcgcaggccg ggacggcgca
     1261 agcgtttgag aacgaacagc caccgggcga aattgggatc gatgacgaca ctgtgtccgc
     1321 caagctgcag gaagaaactg gcgtggccga taaaggtgag gcccagctcg ccgttggtga
     1381 ccaggcgcgg cttatgcgtc tggccgcagc gtggagttac cgcagagtga cgaaccagcc
     1441 ttccgaactg ggtggcccgc ttgcgaaggg ccggattgcg cagcgtttgc ttgatcatcc
     1501 tgttcgagag ccgggactga cggtccggac tcgagtctgt tcaaatacta gtcccgacgg
     1561 cgcttccggc aatagcaata gcggtccgtc gggccttttt aaccaggcat ttatcttaga
     1621 tgagcagggg agcacccggg ctgcattttt gatcctgggc ggtaccgccg taaccttgtt
     1681 ctaaaaaccg gaaccagcgg ccagtcccac gcttgaccac ggttcgagag gtcatataat
     1741 cccaacacgt tcatttttct cattggggtt cagcgggcaa aaggagagct ctgcatgcga
     1801 gtggcatttc tggggttggg aatcatgggg cagccgatgg cagcgaatct ggtgaaggcg
     1861 gggcacgaag tcacagtgtg gaaccgcacg ccggggaagt tggtggaagg cgcggcggtg
     1921 gcgcccacac cggccgcggc tgcgcaggga gcggaagtcg tgtggatgtg cgtttccgac
     1981 accgacgcag ttgagaatgt gctgttcggg cccgatggcg ccgagtcgtc gctggcggaa
     2041 ggaatgatca tcgcggactc gagcacaatc tcgccttcgg ctacgttgaa gtttgccgag
     2101 cgcgtgcggg cgaagggcgt ggcctgggtg gacgctccga tgacgggctc gaagatcggc
     2161 gcgcgcgacg ggacgctggt gttcatcgta ggcggagatg aagcggtcat cgagcggctg
     2221 aagccattat ttgcggcgat gggaaaaaag atcttccgca tgggcgagac cagcaagggc
     2281 caggcgacga agctggcgat gaatctgcaa atcgcaatga tctttgaagg cttcgcggag
     2341 gcgctgacgc tggcgaccaa gctgggagtc gatgcccagc aattgatgtc gctcatcgat
     2401 gccacgatgg tccgctcagg cgtcgtcgac tacaaggcgc cgttcatttt gcagcgcgac
     2461 ttcacgccga actttccgct gcgcctgatg cacaaagata tccggctgac gctcgaagcg
     2521 gcgaaagaag cccgggtgaa gctgccgggg ctcgagaccg tagaagaaat ctacgagatg
     2581 gcgaccgagg atggtcacaa ggatctcgac tacgccgcca ccctgacatt gcttgaaaaa
     2641 tgggcgggtg tgcaggtcaa gggcaaggcc gcggcctgag agtgggctgg agctctccca
     2701 gttcgttact cccgtaatgc gcgaaaaggc gctattacac aagtattaca ctcgtccccg
     2761 ccgttctctt ggtagcttag ctgtggcttg gccgaagttc actacgaaaa cggggaccct
     2821 ccttggaact gcaagacctg gaaaaagtac aaactcctaa agacgaaatc aactggatca
     2881 cagcgatctt catggcgctg tttcacgtgg gagccgtggc ggcgctgttc tttttcacct
     2941 ggaaggcgct gctggtcgcg gttttcttgt ggtgggttgc gggcagcctg ggcatcggga
     3001 tgggctacca ccggctgctc acccaccgcg gatttaagac ttacaagtgg gtcgagtatt
     3061 tcctgacttt ctgcgccaca ctggcgcttg agggcgggcc gattttctgg gtggcgacgc
     3121 accgcattca tcatcagtac tcggatcagg atggcgatcc tcattcgccc atcgacggca
     3181 agtggtgggc gcacatggga tggattctca cgggcaagtc gatgcatcat gacgccacca
     3241 cgctggcgcg ctacgtcccc gatctcgcca aagacaagtt tcatgtctgg atgacgaaat
     3301 atcattacgt cccgcagatt attttaggga ttgcgctgtt cgccatcggc gggctgccgt
     3361 ggctgctgtg gggaactttc ttccgcaccg tgtttctctt gcactgcacc tggctggtga
     3421 attcggcgac gcactcctgg ggctcgcggc ggttcgcgac ccgcgatctt tcgaccaaca
     3481 gctggtgggt ggctttgttg acgttcggcg aaggctggca caacaaccac cacgcgcatc
     3541 cgacgtcggc ggcgcacggc ttcacttggt acgagattga cacgaactgg tacgggatct
     3601 ggctgctgaa gacgttcggg ctggcgtggg acattaaacg ggtaagcctg gccgacattg
     3661 agcgtgggct ggtcgccgcg gccaacgggc ggctgatcgc accctcgaac gcggcggagt
     3721 tttgggccgc gggcgactag ccgctgcgcg gaaaccattc atgtttttat cagcattagg
     3781 gcgtgtaagc agcgctttga tacttgtctt gctattcgca gggctagcgt gcgagaggac
     3841 ggcggggcca cgagtctcgg cgcaggtccg gacgggcgct ttctatgccg tgttcggcgg
     3901 tcctaacagg gttcctgagc gcatcagttc gcacgttgcc tccaatctcg atccttccca
     3961 tgaacgcttc tttctgtacg ttccatcgag ctacaccggc accgaatcgt acgggatgat
     4021 tgtcttcacg tatgccgatc cggaagcgcg actgccctat ggctggcaac aagttttaga
     4081 ctcacgcaaa tacatttttg tcgctgccca aaatgcggga aatgaccagc cgcgggggcg
     4141 aaggctcgga cttgccgtga tgggagctct taagacgatg aaggcgtaca acattgaccg
     4201 caatcgcgtt tacgccagtg gcttctcggg cggtgcacga atggctggtc ttctcgcttt
     4261 ctaccaatcc gatatcttcc acggtactat ccaaaattgt ggcgccgatt tttaccggca
     4321 agtgccaacc gtaaatgcgg tctcgcaagt cgatacggca ggccaagctt atggggtact
     4381 tgaagcctcg caagatgaaa ttcagcgggc caaaacgcta cgcttcgtgt tgatcaccgg
     4441 caccaacgac tttcgtcgcg ggaacatcct ggacatcttc catggtggat ttgagcgcga
     4501 tggtttccac gccaaactat tcgacatggc gggaatgggg cacgacatct gcgacagcga
     4561 aacgttgact cacgccctcg atttcctgga aagcggttcg taaaacgcgc aatcctgggg
     4621 ctgctcgtgg ccagcgtttc ctggtagcgg atagaatgac gaccctcaga ggctaaagcc
     4681 cgtttttttc tgcccgtatc ggcacggctg aagccgtgcc cttcccaaac cacttacgaa
     4741 gtagcgtcta gctgttcatc actttctttg catcgtctgc actgggcttg cgctctgcgc
     4801 cgaggtcacc gctttctgct gcgccaccca tccgtgaatg cggttgccca gaacgtcgag
     4861 cggcagcgct ccgcccgaca gcacctcgtc gtggaaggcg cggacatcaa acttctcgcc
     4921 tagctgatcc ttcgaatact ggcgcagctt tagaatctca agctggccga ccttgtagcc
     4981 gagagcctgc gccgggttcg ccatatagcg gtcggtttcc tcctgcactt cgggctcgtc
     5041 gatggcggag tgctcgtgga agaagtcgac tacctgctgc cgcgaccagt gcttgtagtg
     5101 gaatccggta tcgaccacca gccggatggc gcgcagcatg tcatcctgca agtggccgta
     5161 ataactgtac ggatcctgaa agaatcccac ttccttgccc agttcctcgg agtagagcgc
     5221 ccagccttcg gtgtaagccg tataaaactc gtgctggcgg aaggggggca actcgggcag
     5281 ttcctgcgcg atcgaaatct gcagatgatg gccgggaacg ccctcgtgat aggccgtggt
     5341 ctcgatgtcg agcgtggtgc ggttctggaa atcgccggtg gcgaccatga cgtgcgcctt
     5401 gcgcgaaccg tccggcgtgc cttcaaggta gtgtgttgag gcttccttct cgcgatactc
     5461 ctcgatcggc ttcacgtcga gatgctcttt cggcaggcgt ccgaaaagct cggggagctt
     5521 ggtgtacatc tggtcgatgt atttctggta gagatcgaga atctgctggc gcgactgggg
     5581 atggagcgca gggttggcct tgatcgatcc gtagaagctc ttcaaatctg gaaatccgag
     5641 ctgccgtgca acgcctgaca tacgttcttc gatagccttc acctgggcga ggccgatctg
     5701 gtgaatttct tccggcgtca ggttggtggt cgtgacttcc ttgacgcgga aggcatagcg
     5761 cgcatcaccg tcgggaagtg cccacactcc gggctcgctg cggcccttgg gcgcgtactc
     5821 gtcacgaacg aaagcggtga acttgacgta ggcggggatc accgagtcgc gaatcg
//
LOCUS       DASPUL010000387         3164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_903719, whole genome shotgun sequence.
ACCESSION   DASPUL010000387 DASPUL010000000
VERSION     DASPUL010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903719"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VEJ00_09455"
     CDS             complement(<1..646)
                     /locus_tag="VEJ00_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01427.1"
                     /translation="MLAPFKAPLLLIGVTAAICIVALLLPPIPQPQSYHNFADQREWL
                     SIPNFGDVVSNLPFAILGIWGLIFLAGSARRFSDPRERWLYSVVFAGLVLTGAGSAYY
                     HLAPDNTRLVWDRIPIMITFMALLAAVIAERLSGNAGVWLFPVLQAAGVGSVLLWRAG
                     ELGGYGDLRFYAAVQVYAILVLLLALLFPAKYTRGGDFAVIVGFYVLAKILEESD"
     gene            complement(667..1257)
                     /locus_tag="VEJ00_09460"
     CDS             complement(667..1257)
                     /locus_tag="VEJ00_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01428.1"
                     /translation="MYRDPEAAERGVGYLAAFIYSIVVALIWLPVAALRRAARLLREF
                     GVESWPRTDGSITSGNVKVIHGWILDYALGRLDYSYRVAGEYYAGAIVGQYPDEQAAW
                     DFVDAYRDRPVVVRYKDNKPEVSVLRDADQDLTWTGTGPGKDFFTMIWQHWRDELRGE
                     QQPLADEEDSPTDKPFHRRRRALGPLRSTAHPSRFA"
     gene            1327..2433
                     /locus_tag="VEJ00_09465"
     CDS             1327..2433
                     /locus_tag="VEJ00_09465"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01429.1"
                     /translation="MIVTVRSFAKINLGLRIGPARADGFHELLTVYQTIGLHDIIRVG
                     VRRGTGVEIRCADPRVPTDESNTCFRIVGLAMRALTAKARVTIEIEKRLPVQGGLGGA
                     SSNAVAALLGLERALKKSLPLVHKLRIAAEVGSDLPLFLVGGTVLGVGRGEQVYPLPD
                     LPAIACVLVTPEVGISTPKAFVQWDRLCGAGAPAREVVAAKQVVEDVLGEHSVEARLT
                     STGEGARSRTATSDPPGKLTSPTSSDRMNELGRGLSAWLSEPYSSAPSRLLKRRGRAG
                     NPLLWLVRAGIENDFEQVVFPECPELREGKRALERAGAKYASLSGSGSALYGLFASRA
                     AAGQAAARLREQGWAAHATTTVPRRQYWRRIFDC"
     gene            2640..2713
                     /locus_tag="VEJ00_09470"
     tRNA            2640..2713
                     /locus_tag="VEJ00_09470"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2672..2674,aa:Gln,seq:ttg)
     gene            2836..>3164
                     /locus_tag="VEJ00_09475"
     CDS             2836..>3164
                     /locus_tag="VEJ00_09475"
                     /inference="COORDINATES: protein motif:HMM:NF025165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HXX01430.1"
                     /translation="MATLVTPTDKAEKVEKKSPPVTDEKPERKPRQRVDDKFKIFSGT
                     ANEALADEVCAFLGMTRGQAQVIRFRDGECYVQIQENVRGADVFVMQPTCRPVDEHLM
                     ELLLMIDA"
BASE COUNT          662 a          918 c          984 g          600 t
ORIGIN      
        1 ggtcggattc ctccagaatc ttggccagga cataaaagcc cacgatcacg gcgaagtctc
       61 cgccccgcgt gtactttgcc gggaacaata gcgccagcag cagcacaaga atcgcataca
      121 cctgcactgc ggcataaaaa cgcagatccc cgtagccgcc aagttcgccg gcccgccaca
      181 gcagcacgct gcccacaccc gcggcctgca agacgggaaa cagccaaact cctgcgttcc
      241 cactcagccg ctccgctatc actgccgcaa gcagcgccat gaacgtaatc atgatcggaa
      301 tgcgatccca aaccaggcgg gtgttatcgg gcgcgaggtg gtagtaggcc gatccggcgc
      361 cagtgagcac cagtcccgcg aagacaacgc tgtaaagcca gcgctcgcgc gggtcggaga
      421 accttcgcgc agatccggcc aggaagatca ggccccatat cccgaggatc gcgaagggaa
      481 ggttcgagac cacatcgcca aagttcggaa tgctgagcca ctcccgctgg tcagcaaagt
      541 tgtggtatga ctgcggctgc gggatcggcg gcagcagaag cgcgacgatg cagatggcag
      601 ctgtgactcc gatcagaagc agcggcgctt tgaatggagc gagcatgcgc tatgctgatc
      661 aggatcttac gcaaatcggc tggggtgtgc ggtggatctc agcggcccaa gcgctcttct
      721 gcgccggtgg aagggtttat cagtgggaga gtcttcctca tctgcaagag gctgctgttc
      781 gccacgtaat tcgtcccgcc agtgctgcca gatcatcgtg aagaaatcct tgccaggccc
      841 ggtcccggtc caggtaagat cctgatccgc gtcgcggagc accgatacct cgggtttgtt
      901 gtctttgtat cgcacgacaa cgggccggtc gcggtaagcg tcaacaaaat cccatgcagc
      961 ctgttcgtca ggatattggc cgacgatggc gcccgcatag tattcgccag cgacgcgata
     1021 gctatagtcc aatcggccaa gagcgtagtc gaggatccat ccgtggatga ctttgacatt
     1081 gcccgacgta atcgagccgt ctgtccgagg ccagctttcg acgccgaatt ctctcagaag
     1141 acgtgcggcg cgccgtaggg ctgcaacggg aagccagatc agggcgacaa cgatgctgta
     1201 aatgaaggcc gctagatatc caacaccgcg ttcggcagcc tcgggatcgc ggtacatata
     1261 gatgggtgag agtataccct cgcgtgagtg cttcgaaagc cgcagtgtga ttaaatgcgg
     1321 ttgcccatga tcgtcaccgt ccgttccttc gccaagatca acctcggcct ccgcatcggc
     1381 ccggcgcgcg cggacggatt ccacgaactg ctgacggtct atcagaccat cggcctgcat
     1441 gacatcattc gcgtcggggt ccgccgtggt acgggagttg agattcgttg tgccgaccca
     1501 cgcgttccca cggacgaatc gaatacctgc ttccgcatag tcgggctagc gatgcgcgcg
     1561 ctgacagcca aagcgcgcgt caccatcgag atcgaaaagc gcctgccggt gcagggagga
     1621 ctcggtggag cttcctcgaa cgctgtcgcg gcgttgctcg gactggagcg agcgctgaag
     1681 aagtctctgc cgcttgtcca caaactgcga atcgcagccg aggtcggatc cgacttgccc
     1741 ttgttcctgg tcggcggcac cgtgctcggc gtggggcgag gggaacaggt ctatcccctg
     1801 ccggatctgc cagccatcgc ctgtgtgctg gtgacgccag aggtagggat atccacgccg
     1861 aaagcgtttg tgcagtggga ccgtctgtgt ggcgcgggcg ctcccgcccg cgaggtcgtg
     1921 gctgctaagc aggttgttga ggacgtcttg ggcgaacact ccgtcgaggc ccgcttgacc
     1981 tcgacgggcg agggcgcccg ttcccgcacg gccacttctg atcctcccgg aaaattgacc
     2041 tcgccgacaa gctccgatag aatgaatgag cttggccgcg ggttgtcggc gtggctgagc
     2101 gagccttact ccagtgctcc ttctaggctg ttgaaaagaa ggggccgggc cgggaatccg
     2161 cttctctggc ttgtccgggc tgggatcgag aacgacttcg aacaggtcgt ctttcctgag
     2221 tgtcccgagc tgcgtgaagg caagcgtgcg ctggagcgcg cgggcgcaaa atatgcgtcc
     2281 ttgtcgggct ccggatcggc cttgtacggg ctgtttgcgt cgcgggcggc ggcagggcaa
     2341 gctgcagcca ggctccggga gcaaggctgg gcagctcatg ccaccacgac ggtgccacgg
     2401 cggcagtact ggcgcagaat ttttgattgt tgatttttga ttgttgaatg aaaatgaatc
     2461 tggcccccgc gggggtttac tcaacaatta acattacatc aatcgacaat tgagtactgg
     2521 gccgtcgact aatggtaggt tgaggcgctt tgcgggcgtg agcgaagcgg gagcccgcag
     2581 ccgaaatcct gagcgttctt cgcgaaggat ctcaaactcc aacagtcacg tgccgagact
     2641 gggccgtcga ctaatggtag gtcaagtgcc tttggagcac tttgtctagg ttcgaatcct
     2701 agcggcccag ccagaaaatc agcttttggc tcttagctct tggctgttag ccgaaccggg
     2761 cgcgagccga aagcgattca agccaagagc caaaggctaa gagctaagca agccaggagc
     2821 cctgagacaa aaaccatggc tactttagtc acgccgaccg acaaagcaga aaaagttgag
     2881 aaaaagagtc cacccgtgac cgacgaaaag ccggagcgca aaccgcgcca gcgcgtggac
     2941 gacaagttca agattttcag cggcacagcc aatgaggcgc tggccgacga ggtttgcgcc
     3001 tttctcggca tgacgcgcgg acaggcgcag gtcattcgct tccgcgacgg cgagtgttat
     3061 gtccagattc aggagaacgt gcgcggcgcc gatgtgttcg tcatgcagcc cacgtgtcgc
     3121 ccggtggatg agcatctgat ggagttgctg ctcatgatcg acgc
//
LOCUS       DASPUL010000388         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1991308, whole genome shotgun sequence.
ACCESSION   DASPUL010000388 DASPUL010000000
VERSION     DASPUL010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991308"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VEJ00_09480"
     CDS             <1..594
                     /locus_tag="VEJ00_09480"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXX01431.1"
                     /translation="NAPVEKVQTGGFGDPNGLPGTGKQGAKLYAASMGSFDMPAGPGQ
                     GNGSGGANGIKGTVASAGFGNGIANGGHGDGRSNGQGVSTGGFGSQTVAAHQGPKIAA
                     PDAGAATTPVEITYKPNPVYTDEARALKLEGEVLLEVSFSATGSLHVNKVVRGLGHGL
                     DEAAIAAANKIRFKPALRNGQPVDSTAIVHVMFQMAY"
     gene            687..2564
                     /locus_tag="VEJ00_09485"
     CDS             687..2564
                     /locus_tag="VEJ00_09485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXX01432.1"
                     /translation="MRTWNWTLTAALLGSSFLGITGAAAQQADGTQPAAQSPAPDATQ
                     TAQPSQPAATAATQTAQAAAASAPSSSPSTTSVPASSASAGTAPVAPAPMAAPTTMDQ
                     VVNLFIEREHGLIKALSNRTPVVETYLQNLTSDPQLGPVPSEDHYFLGRLDMGETVDR
                     KDYLKEEEKGMQTRLLGGLQKLYKVQYQPMGFSWMVYADRTDFDREHYNFSYIRREFL
                     GEVRCLVFDVKPKPKSGRGRFLGRIWVEDQDFNIVRLNGTYYPAPRNTYFFHMDSWRL
                     NLIPGYWVPAFIYSEEGDFSAGVKNKLAFKAQTRIWGYDLKKGDKDDELTQIRVDAVK
                     DESPAAQDASPLQAEREWQQQAEDNVVERLTRAGLLAPEGDLDHILQTVVNNLEVTNN
                     IDLPRPVRTRVLLTSPLETFSVGNTIVISRGLVDVLPDEASLAAVLSHELAHIVLGHN
                     LGSKYAFNDRMLFSDEGTYQNLGFKHIPEEEQAADKKAVDLLKNSPYAQKLDNVGLFL
                     KALQQRAPALGALLTTHLGNPLAEGGQVNRLQALESQGPALDMNKLDQIAALPLGGRV
                     KINPWDDKAEMVKASPVAITSARDKMPFEVTPFYPRLARYGAGATNATPTTASNSNSG
                     N"
     gene            complement(2745..3188)
                     /locus_tag="VEJ00_09490"
     CDS             complement(2745..3188)
                     /locus_tag="VEJ00_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXX01433.1"
                     /translation="MKLDRISAEQVREKVQRFWHAFSGKARTEFEAMYLPSATVFAVD
                     GRRSEVARLTVVRRARELMSQSSSVRARLGSIEVQVLGPDVAVTSYPLHFSTVRSMPN
                     GRRYRSDIPYLRATHIFVRDAEGQLRIIHEHLSSAEPVNVQEVPQ"
     gene            complement(3229..3660)
                     /locus_tag="VEJ00_09495"
     CDS             complement(3229..3660)
                     /locus_tag="VEJ00_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXX01434.1"
                     /translation="MERISPEQVRSQVQKFWQSLSGKSNESFDDLYSPDATVITGKAR
                     RAERAQLAVARRKREIAQTSEASVELGPIDVEVVGDVAIASYTYDFMRVRKRADGAVR
                     RHTPQGRATHVFQRDAEGRLRIAHEHLSSAAPTGVQPMEKD"
     gene            complement(3730..>3919)
                     /locus_tag="VEJ00_09500"
     CDS             complement(3730..>3919)
                     /locus_tag="VEJ00_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01435.1"
                     /translation="LDEPQRQQIVAESRHALKAQGSLLVFQYNRTLLPYLKSSFSSVK
                     LGYQLFNILPALIFHCTP"
BASE COUNT          788 a         1201 c         1253 g          677 t
ORIGIN      
        1 aacgctccgg tcgagaaggt gcagaccggc ggattcggcg atcccaacgg attgccgggc
       61 acgggcaagc agggcgcgaa gctgtacgcg gcatcgatgg gatcgtttga catgcccgcg
      121 ggtcccggac agggcaacgg atcgggcgga gcgaatggaa tcaagggcac ggtcgccagc
      181 gccggttttg gcaacggtat cgccaacggc ggccatggcg acgggcgcag caacgggcaa
      241 ggcgtctcga ccggagggtt cggatcgcag acggtagcgg cgcatcaggg gccaaagatc
      301 gcggcgccgg acgcgggtgc ggccaccacg ccggtggaga tcacctacaa gccgaatccg
      361 gtttacacgg atgaggcgcg cgccctgaag ctcgaaggcg aggtgctgct ggaggtgagt
      421 ttctcagcca ccggttcgct gcatgtgaat aaagtggtgc gcggcctggg ccacgggctg
      481 gatgaagccg cgatcgccgc cgccaacaag atcaggttca agccggcgct gcgcaacggg
      541 cagccggttg attcaaccgc catcgtgcac gtgatgtttc agatggcgta ctgaaaagca
      601 gtacccagtg gtcagtaccc agcggtcagt gagttcgttg agtactggta ctgacaactg
      661 acaactggtt tttggagaag agagtcatgc gtacttggaa ttggacattg acggcggccc
      721 tgctcggatc gtctttcctg gggatcacgg gcgcagccgc ccagcaagcg gacgggacgc
      781 aacccgccgc gcagtcgccg gcgccggatg cgacgcagac ggctcaacct tctcagccgg
      841 cagcaacggc ggcaacccag acggcgcagg ccgctgcggc ttccgctccg tccagctcgc
      901 cttcgactac gtccgttccg gccagttcgg ctagcgccgg cacggctccg gtagccccgg
      961 ccccgatggc cgctccgact acgatggatc aggttgtgaa cctgttcatc gagcgcgagc
     1021 atggcctgat caaggcgctg tcgaaccgca caccggtggt cgagacctat ctgcagaatc
     1081 tgaccagcga cccgcagctc ggtcccgtcc ccagcgagga tcactatttc ctcggccgcc
     1141 tggatatggg cgagaccgtc gaccgcaagg actatctgaa ggaagaagag aagggcatgc
     1201 agacgcgcct gctgggcggg ctgcagaagc tctacaaggt gcagtatcag cccatgggct
     1261 tctcctggat ggtctatgcc gaccgcaccg acttcgaccg cgaacactac aacttcagct
     1321 acatccgccg cgaatttctc ggcgaagtgc gctgcctggt gtttgacgtg aagcccaagc
     1381 cgaaatcggg ccgcggccgc ttcctgggcc gcatctgggt cgaggaccag gacttcaata
     1441 tcgtgcgcct gaacggcacc tactatcccg cgccgcgcaa cacctacttc ttccacatgg
     1501 acagctggcg gttgaacctg atccccggct actgggtgcc ggccttcatc tacagcgaag
     1561 agggcgactt cagcgccggc gtgaagaaca agctggcctt caaagcacag acccgcatct
     1621 ggggctatga cctgaagaag ggcgacaagg acgatgaact gacgcagatc cgcgtcgacg
     1681 ccgtgaaaga cgaaagcccg gcggcgcagg atgcatctcc gctgcaggcc gagcgcgagt
     1741 ggcagcagca ggcggaagac aacgtcgtgg agcgcttgac gcgggcgggc ctgctcgctc
     1801 ccgaaggcga cctggaccac attctgcaga ccgtggtcaa caacctggaa gttaccaaca
     1861 acatcgactt gccgcggccg gtgcgcacgc gtgtactgct cacctcgccg ctcgaaacct
     1921 tcagcgtggg caacaccatc gtgatcagcc ggggcctggt cgacgttctg ccggacgagg
     1981 ccagcctggc ggcggtgctg tcgcatgaac tggcgcacat cgtgcttggc cacaacctgg
     2041 gctcgaagta tgccttcaac gaccgcatgc tgttctccga cgagggaacg taccagaacc
     2101 tgggattcaa gcacatccct gaagaagagc aggcggcgga caagaaagcc gtcgacctgc
     2161 tgaagaactc accctacgcg cagaagctgg acaacgtcgg tttgttcctc aaggcgctgc
     2221 aacagcgggc tcctgcactg ggcgccctgt tgaccacgca cctgggcaat cctctggccg
     2281 aaggcggaca ggtcaaccgc ctgcaggctc tggaatcaca gggcccggcg ctcgacatga
     2341 acaaactcga tcagatcgcg gcgctgccat tgggcggtcg cgtgaagatc aacccgtggg
     2401 acgacaaggc ggaaatggtg aaagcgtctc cagtcgcgat cacgtcggcg cgcgacaaga
     2461 tgccgttcga ggtcacgccg ttctatccgc ggctggcgcg gtacggcgct ggggcgacaa
     2521 acgccactcc gaccacggcg tccaactcga actcgggcaa ctgaaattca atccccactt
     2581 ctcgcgcaga gagcgcgcga gaaatggggc acgtaacaag ctttcctctc caagttgaag
     2641 gaagacgtgg gccggcatcc ggaagcaatt ccggaatgcc cggccttcgt ttgttagggg
     2701 ccgtgccaag aacgtgtggc ctttgcgggg gtgagtttca ggatttactg aggcacttcc
     2761 tgcacattga ccggttcggc cgaactcaaa tgttcatgaa taattctgag ctgaccctcc
     2821 gcgtctcgca cgaaaatgtg agtcgcgcgc aggtagggaa tgtccgaccg gtagcggcgg
     2881 ccattcggca tgctccggac agtcgagaaa tgcaacgggt aggaggtcac ggccacgtca
     2941 gggccaagaa cctgtacttc gatgctgccc aatcgggccc taactgaaga ggactggctc
     3001 atcagttccc gagcccggcg cacaaccgta aggcgcgcca cctcgcttcg ccgtccgtcg
     3061 acggcaaaca ccgttgccga gggcagatac atggcctcaa attcagtccg ggccttgcca
     3121 gaaaaggcat gccagaagcg ctggactttt tcccgtacct gctcggccga aattctgtcc
     3181 aatttcatcg cgggatctgg cacccgcccg ggtcagcgtg cgtcatgcct agtccttctc
     3241 catgggttga acaccggtgg gagccgccga gctaaggtgt tcgtgagcga tgcgcaggcg
     3301 gccctcggcg tcccgctgga aaacgtgcgt ggcccgtcct tgcggagtat ggcgtcgaac
     3361 cgccccatcc gcgcgcttgc gaaccctcat gaaatcgtag gtgtaggagg cgatggcgac
     3421 gtcgccgaca acttcgacgt cgatggggcc cagttcgacg ctggcctcgc tggtctgcgc
     3481 gatctcccgc ttccgtcgag ccactgccag ctgcgcccgc tcggctctcc tggctttgcc
     3541 ggtgatcaca gtcgcgtcgg gggaatacaa atcgtcgaag gactcgttcg acttgcccga
     3601 cagggactgc cagaatttct gcacctgaga ccgcacctgc tcgggcgaga tgcgttccat
     3661 gacggcatca gcatatcacc ggacaaggct tgtatgggtt tgaccaacga cgatggttgt
     3721 tacgtcccct cagggggtgc agtggaagat cagcgcgggc aggatgttga agagctggta
     3781 gccgagtttg accgagctga agctggattt cagatacggc agcagcgtgc ggttgtactg
     3841 gaagaccagc agcgagccct gcgctttgag cgcgtggcgg gattcggcga cgatctgctg
     3901 gcgctgcggc tcgtccagc
//
LOCUS       DASPUL010000389         4569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_213494, whole genome shotgun sequence.
ACCESSION   DASPUL010000389 DASPUL010000000
VERSION     DASPUL010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213494"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..570)
                     /locus_tag="VEJ00_09505"
     CDS             complement(148..570)
                     /locus_tag="VEJ00_09505"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HXX01436.1"
                     /translation="MSQGGRWFAALVFAAGAVAALLLRIFDPATSGVFPPCPVRYLTG
                     WYCPGCGSLRAIHQLLHGNLQAAWAMNPLAVILLPFLLYGLASHVLLEIRGRGLPQTF
                     LPAVWIRALCVVIILFGVARNLPLHPLNLLAPGAMLHP"
     gene            complement(583..966)
                     /locus_tag="VEJ00_09510"
     CDS             complement(583..966)
                     /locus_tag="VEJ00_09510"
                     /inference="COORDINATES: protein motif:HMM:NF016394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CD225/dispanin family protein"
                     /protein_id="HXX01437.1"
                     /translation="MFCPQCGANNADTAAVCVQCGRSLQAATSPAPIQAQGVILPPGA
                     TVPNYLVFAILATVLCCLPAGIPAIVYAAQVNGKLQAGDLAGAQQASKNAKLWCIISV
                     VAFFAGFLVWGLLVMLGILGSFAHH"
     gene            complement(1000..1725)
                     /locus_tag="VEJ00_09515"
     CDS             complement(1000..1725)
                     /locus_tag="VEJ00_09515"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HXX01438.1"
                     /translation="MASVCAACGNSMAEGDRFCRVCGRDSFIGTLATSPITPVDSGAP
                     ATTSGKAIASLVCGLLFFVPLLFIVAIVLGHLALSEIKRSAGRLKGEGLAIAGLVLGY
                     GWVLTIPIILIIAAIAIPNLLRARMAANESSAVASVRTLNVAEVTYSSQHPDAGFTCS
                     LADLSGQIDNKLASGRKTGYVFELSGCTAGENGAIVKYQLVAYPMTRNQTGIRAFCSD
                     ESGVVKVDANGSAQDCIDHGSNL"
     gene            1888..2349
                     /locus_tag="VEJ00_09520"
     CDS             1888..2349
                     /locus_tag="VEJ00_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HXX01439.1"
                     /translation="MPMLCLHCSAQMPDTAAFCPGCGRPTQAGVRARQKAGFLPENIA
                     GALAYLTFIPAIVFLVRQPYNRNRFVRFHSIQCLLLWAFVLVAIAVLKITGVILFNVP
                     VAGPLFVVLLSVAAVIGACLIWLVLVIKAAQGQMFKLPLIGPLAEHYAAAQ"
     gene            2477..2908
                     /locus_tag="VEJ00_09525"
     CDS             2477..2908
                     /locus_tag="VEJ00_09525"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HXX01440.1"
                     /translation="MAFCSTCGTQIPDGSTTCAAHAAPAAPAASTGGMTDNVAGMLAY
                     ITIIPSIIFLVLEPYNKNRFVRFHAFQNLFFAVAWFVLWFALMFIGHIPFLGWMTILL
                     WPLIGLGVFILWIIMLLKAYQGQMWKLPVIGDMAEKQANAM"
     gene            3166..4134
                     /locus_tag="VEJ00_09530"
     CDS             3166..4134
                     /locus_tag="VEJ00_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01441.1"
                     /translation="MRVRVFYHDKCFDGASSAALFARFYRERIRSDADFVFTGLVHRA
                     GALFDERQFDGDENAIVDFKYSSSPKITWWFDHHQSAFLSPADAEHFEQNQSNRKFYD
                     PDFKSCTSFIAMIAQQHFGFDPSPVADLVHWTDVIDGALYPDAKTAVEMEAPAMKLTM
                     VIESAPDHGFVPNLIPLLATKPLAQILEEPFVAPLLPPLLKRHEVSIGILRQRAECKD
                     RTIFFDITDHDLEGYNKFIPYYLHPESVYSVGLSKSSFRVKVSVGSNPWAPEEPEVNL
                     AKICERYGGGGHARVGAISFNVNQHAAAIKASQEIVAELRAAIRKK"
     gene            complement(4331..>4569)
                     /locus_tag="VEJ00_09535"
     CDS             complement(4331..>4569)
                     /locus_tag="VEJ00_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01442.1"
                     /translation="NEHQAMLINSGLASGKRYGFVFTLSECSGRPATGFHLTASPNAN
                     TYGRKAFCADQSKVIRSSDDGNAATCLASGTPVP"
BASE COUNT          865 a         1438 c         1339 g          927 t
ORIGIN      
        1 tctcgccgga agtcagccag ctcgagcagg agatcggccg ccgctatcac tacctcacca
       61 cgcgccatac gttccagatc tacgggctgt gcgaggagtg ccggaagaca cgccaggcca
      121 gctaggctcc ggtgctcgtt ccgcgattca cgggtggagc atcgcgcccg gcgccagcag
      181 attgagcgga tgcaaaggga ggttgcgcgc gactccaaac aggataatca ccacgcacag
      241 cgcgcgaatc cagactgccg gcaggaaggt ttgtggcaat ccccgcccgc gaatttcgag
      301 cagcacgtga gaagcgagtc cgtagaggag gaacggaaga aggatcacag cgagcggatt
      361 cattgcccag gcggcttgca ggttcccgtg cagaagctgg tggatcgccc gcagcgagcc
      421 gcatcccggg caataccagc ccgtcagata ccgcaccgga caaggcggaa aaaccccgga
      481 cgtagccgga tcgaagatgc gcagcaagag cgccgctacc gccccggcgg cgaagaccag
      541 cgccgcaaac catcgcccgc cctgcgacat ccccgtactc tcctagtggt gggcaaagct
      601 gcccaggatg cccagcatga cgagcaggcc ccagacaagg aatcccgcga aaaatgctac
      661 caccgaaatg atgcaccaca gctttgcatt cttcgacgcc tgctgcgctc cggccagatc
      721 gccagcctgc agcttgccgt tcacctgcgc cgcgtagacg atggcaggga tccccgccgg
      781 aaggcagcaa aggacggtgg cgagaattgc gaacaccaga taattcggca cggttgcgcc
      841 cggcggcagg atcacgcctt gagcctgaat aggcgccgga gaggtagctg cttgaagaga
      901 tctgccgcac tgtacgcaca ccgccgctgt gtccgcgtta ttggccccgc actgaggaca
      961 gaacattcgt ttttcctccc gtaatacttc acgcgatatc tacaggttcg atccgtggtc
     1021 gatgcagtcc tgggctgagc cgtttgcgtc gaccttgacg acgccggatt cgtcggagca
     1081 gaacgcgcgt attccggttt gattcctggt catcggatag gcgacgagct gatacttcac
     1141 gatcgcaccg ttttcgcccg ccgtgcagcc ggacaattcg aacacgtatc cggtcttccg
     1201 gccactggcg agtttgttgt cgatctggcc cgagaggtcc gccagcgagc aagtaaagcc
     1261 ggcgtccggg tgctggctcg aataggtaac ctcagcgacg ttgagcgtcc gcaccgatgc
     1321 cacggccgac gattcgttgg ccgccatccg cgcccgcaga aggttcggga tcgcaatcgc
     1381 cgcaataatc aggatgatcg ggatggtcag aacccagccg tagcccagta ccagtcccgc
     1441 gatggcgagg ccctcgccct tgagccggcc cgcacttcgt ttgatctccg acaaggcaag
     1501 gtggccaagc acgatggcca caataaagag caaagggacg aagaacagaa gtccgcacac
     1561 caggctggcg atggctttgc cgctggtggt tgcaggcgca cctgagtcaa cgggagtgat
     1621 gggcgacgtt gcgagtgtgc cgatgaaaga gtctctgccg cacacccggc agaagcgatc
     1681 gccttccgcc atgctgttcc cgcacgccgc acagactgaa gccattcact ctcccgcgcg
     1741 aaacaccgct gctgtctagc gttgaaattt gcacgaacat tacacttcgt tctgtgccac
     1801 gtcaatgcgt tagattggtt cctggggccg tttgccgcgg cggacttgct tctgataacg
     1861 tgcgccgcag gccaaggttt ggatctcatg cccatgctgt gcctgcactg ttccgctcag
     1921 atgcctgaca cggcggcctt ctgccccggc tgcggccgtc ccacgcaggc cggcgtgcgg
     1981 gcacggcaga aagcaggttt cctgccggaa aacattgcgg gggcgcttgc ctacctgact
     2041 ttcattcccg caatcgtttt cctggtgcgc cagccgtaca accgaaaccg cttcgtgcgc
     2101 tttcactcca ttcaatgcct cctgctgtgg gcgtttgtgc tggttgccat cgcggtgctg
     2161 aaaatcaccg gcgtgatcct tttcaatgtt ccggtcgccg ggccgctttt tgtggtgctg
     2221 ctctcggttg cggccgtgat tggcgcgtgc ttgatctggc tcgttctcgt catcaaagcc
     2281 gctcaggggc agatgttcaa gcttccactg atcggccctc tcgccgagca ctatgcggcg
     2341 gcgcagtagt tctccccctt ccaaatttga ttgcttcggc ccagcttttt tggtacgttg
     2401 cccgcgacgc cgccgaggct cgggctggtg ttgcctgcaa ttcagctcgt ctttggcggc
     2461 tgagggggat accactatgg ctttctgcag cacgtgcggt acccagattc ccgacggcag
     2521 cacgacctgc gcggcgcacg ccgcccccgc cgctcctgcg gccagcacag gcggcatgac
     2581 cgataacgtg gccggcatgc tggcctacat cacgatcatc ccgtcgatca ttttcctggt
     2641 gctggagccc tacaacaaga accgcttcgt gcgcttccat gcgtttcaga atctgttctt
     2701 cgccgtagcc tggtttgtgc tgtggttcgc gctcatgttc atcgggcaca ttccgttcct
     2761 gggatggatg acgatcctgc tttggccact gattgggctg ggtgtcttta tcctctggat
     2821 catcatgctg ctgaaggcct accagggaca gatgtggaag ctgcccgtca ttggcgacat
     2881 ggcggaaaag caggcgaacg cgatgtagcc ggcaggccgt gccaatgtgg ccgcttcaga
     2941 agatgcctgt cgctccaggc gagatccctc gctcgctggt gcaaacgcgc ggcttcgtga
     3001 tgacgccgtc gcacgcctga gactcaactg ctatgcccgc gcgaattcct gcccttgaca
     3061 ttcctctcac aagctgtcaa tcctattagc ttttaccttg ctccccgaac cgcggacgtc
     3121 agtgtgaact cgggagcacg aaactggggg cctttgaggg gttcattgcg agttcgggtt
     3181 ttctatcacg acaagtgctt tgacggagct tcctccgccg cgctgtttgc ccgcttctat
     3241 cgcgagcgca ttcgcagcga tgccgacttc gtcttcaccg gcctcgttca ccgcgccggc
     3301 gcgctgttcg acgagcgcca gttcgatggc gacgagaacg ccatcgtcga cttcaagtat
     3361 tccagttcgc cgaaaatcac ctggtggttc gaccatcacc agagcgcttt tctctcgccc
     3421 gcagacgctg agcacttcga gcagaaccag agcaatcgca agttctacga ccccgatttc
     3481 aagtcgtgca ccagctttat cgcaatgatc gcgcaacagc acttcggctt tgatcccagc
     3541 cccgtggccg acctggtcca ttggaccgat gtgatcgacg gtgccctgta tcccgatgcc
     3601 aagaccgccg tggaaatgga agcgcccgcc atgaagctga cgatggtgat tgaatcggcg
     3661 cccgatcatg gctttgtccc caacctgatt cctttgctgg ccacgaagcc attggcgcaa
     3721 attctcgagg agccgttcgt tgcgcccctg ctgccgccgc tgctcaaacg gcatgaggtt
     3781 tccatcggga tcctgcgaca gcgtgccgag tgcaaagacc gcaccatctt cttcgatatc
     3841 accgaccacg acctggaggg ctacaacaag ttcatcccgt actacctgca cccggaatcg
     3901 gtctacagcg tgggtctcag caagagcagc tttcgcgtaa aggtgtcggt gggatcgaat
     3961 ccgtgggcgc ccgaagagcc cgaagtcaat ctggcgaaga tctgtgagcg ctatggcggt
     4021 ggcggccacg cccgcgtggg agcaatttct ttcaacgtca accagcacgc cgccgcgatc
     4081 aaggcctcgc aggaaattgt cgccgaactg cgcgccgcga ttcgcaaaaa gtagcgccgc
     4141 cgtcggctgg cgagtccccg atcctcttac cgcactgaac gcgaagaaaa cgccgcaaag
     4201 ttcggtaaga aaaacaaccc cgtcggttct gcgtcgcgtg ggtggtcttc tttgcgcgct
     4261 ttgcgcccgt tctcagcgtt ctctgcggga tgagatcgtc ccgctggttg tccgcggacg
     4321 gtgaagcccg ctacggcacc ggcgtcccgc tcgccaggca ggttgccgca ttgccatcgt
     4381 cggaagagcg gatcactttg gattgatctg cgcaaaacgc cttccgaccg taagtgtttg
     4441 cgttcggcga agcggtgaga tggaagccgg tcgcgggcct tccgctgcat tccgaaagcg
     4501 tgaagacaaa cccgtaacgc tttccgctgg ccagtccgct gttgatcagc atggcctggt
     4561 gctcgttcg
//
LOCUS       DASPUL010000390         5669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_622453, whole genome shotgun sequence.
ACCESSION   DASPUL010000390 DASPUL010000000
VERSION     DASPUL010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622453"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(599..1795)
                     /locus_tag="VEJ00_09540"
     CDS             complement(599..1795)
                     /locus_tag="VEJ00_09540"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HXX01443.1"
                     /translation="MSFRRVTGISVFLGVFLEVAGALGTPAVQTPSQPAVLPPAFAGW
                     QMQGPAQTSKDPAAADAASAAILKEYRFSDFASATYTRDDGRTLKIRAARFADASGAF
                     GAYTFYLQREMAKEQIGDQAASVASATGKRVLFYRGHILVDALFSRESPMSAAELREL
                     AGALPRASGNAAVLPPVLLFLPPRGYIANTQKYAEGPLALSALGVQVPADVVDFDSSS
                     EVTTGRYDTPSGEATLVLIYYTNSQMAGAHLRRLDPAHQAEQAGVSVIGNTGSLFYKR
                     TGPIIAIALGPASESDAKSLLGLVNYEASITRNEPTSNREVRDLYLLLLNIVILCAIV
                     GGLAIVAGVAFGGFRILMKRWYPDRVFDRPEQMEFISLHLTETIVQGAPPSSLDKELD
                     GSKKSA"
     gene            1974..2354
                     /locus_tag="VEJ00_09545"
     CDS             1974..2354
                     /locus_tag="VEJ00_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01444.1"
                     /translation="MRTTSVETNQRRGNRTKAVIPVRVKGRDSSGQDFEELAHTLDVT
                     PAGVRLGSVRRELNLLDEITLFYRQRKMQFRVVWTKKLKGTSEFQIGLEAVTQDREAW
                     GMSFPEFKSEVLSRPAVAQASGTV"
     gene            2373..2537
                     /locus_tag="VEJ00_09550"
     CDS             2373..2537
                     /locus_tag="VEJ00_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01445.1"
                     /translation="MGYLPRNLWLDESGQDIAEYSVMLAVILVIVIGTVRLIGSNANN
                     VFSEVGSAIQ"
     gene            complement(2623..4956)
                     /locus_tag="VEJ00_09555"
     CDS             complement(2623..4956)
                     /locus_tag="VEJ00_09555"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXX01446.1"
                     /translation="MKTRSSERRARWGALLLLMALPAVPAYARPHHSESWARDRFAAA
                     ERMREALNGRPPADRTRRDYQRVLNAYRNVYYGAPTSTKADPSVVAVAETLVEMGRRF
                     DDDKILNEAISQYKFLRREYPGSKYRCDALFTIGEIYKDDLDDPDQARATFQEFLHRY
                     PHNRLVEDAQQAIAELDRDSEAEKATEAKKAKSGSDAGKNHTAKDSPANDATGKDSDS
                     RSDASPQETADRHSRRLPRVTGIRHWSTPDYTRVAIDVESDVKFASQRISGPDRIFFD
                     LRDTKLASTLVGKSFDVDDGFLKKIRVAQFQPGRTRVVLEVDDLSDYDAFLLPNPYRL
                     IIDIHGKDIHGKNGHGKSAKDAAGSTTADVEAAARVGAKMETSEASAQTAPEKGEAAP
                     SAAEADSTKVTLDAALKRCAAQNPANSSTCPPAETSLQAKAQPVKKVIVEADDDDVSA
                     VHSKRKSHSLHKPSTATATASAAKPSKNDDASDTEPGGEKKPAANDAVTDPANGKTAS
                     LLASGHSPRDLDVHEARPTARGDRSLIRALGLKIGKIVIDPGHGGHDTGTIGPNGLEE
                     KDLVLDVGRKLGRLLETRLGAEVVYTRKDDTFIPLETRTAIANQQRADLFISIHANSS
                     QDETARGVETYYLNFTSSPEALEVAARENAVSEKSIYELQDLVKKIALKEKIEESREF
                     AGDVQESLHGGLASKSPGLRNRGVKKAPFIVLIGANMPSILAEISFVSNPGDERRLET
                     SEYRQRIADSLYRGIAKYVNGLSGVKVASKMEKASVQ"
     gene            complement(5103..>5669)
                     /locus_tag="VEJ00_09560"
     CDS             complement(5103..>5669)
                     /locus_tag="VEJ00_09560"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXX01447.1"
                     /translation="SAAAEVSQYFVRESAKSANARIIAANSHGHQASPEEVDASIVMA
                     AARHNVDPNLVRAVIKVESNFNSNAVSRKGAMGLMQLMPATARSLNVKNPFDPEQNVD
                     AGVRHLKQLLENYGGDVKLTLAAYNAGSGAVARSAGIPRFAETQNYVRRITNLYYGGF
                     DFSSSGASHDPVTVQRDARGVLYISNTE"
BASE COUNT         1085 a         1774 c         1619 g         1191 t
ORIGIN      
        1 taccgagaat aaacagaaac gcgcccgcca acgcaacgaa tgccgaatag gcgaaccaca
       61 gaatggctag caggtgcacg tgctgctgca cacgacccgg aagtggctgc cccaccgcca
      121 ccggacccat gatttgcttc ccgcacttgc tacaaaagcc ttgccccggt tgtacaactg
      181 caccgcatcc gtcgcagaac atgatggcct cccaatgatc acgaacaggt ccgcaagatg
      241 ctcacaagaa atgatacgaa gaacacgcca aagtgttccg gcggcgcgta ctccggatcc
      301 ctcgcagtgt actccggatg tgcaaccaca gagtgcaggg aaacgaaaag gccacccggc
      361 aactgggcgg cctcttcttc aagggagaat agttccaacg gaaggcaaat tccgtcaaaa
      421 ctttctggcg aaatcttaag agcctaaaaa cgggggtgtc aagcgatttt tgtgaaataa
      481 atgtctttat tatcaataac ttataaaaca agctaacagc gtggcagagt gtaccaattt
      541 gagacaaaat cgccgatttg cccccctaag ccgatacttc taaagccact taccgcggtc
      601 aagccgactt ctttgatccg tcgagttcct tgtccaggct gctagggggg gcgccttgca
      661 caatcgtctc cgtgaggtgc agagagatga actccatttg ctcgggacgg tcgaaaaccc
      721 tatccggata ccaacgcttc atcaaaatgc ggaatccgcc gaaggccacg cccgccacaa
      781 tcgccagacc tcccacgatg gcgcagagaa tcacgatgtt gagcagcagc agatacagat
      841 cacgcacctc ccggttgctg gtgggctcat tgcgggtgat gctggcttca tagttcacca
      901 ggcccaacag cgacttcgca tcgctctccg aagccggacc caaggcaatc gcgatgattg
      961 gcccggtgcg cttgtagaag agtgaaccgg tgtttccgat gacggagact cccgcctgct
     1021 ccgcttgatg cgcgggatcc aggcggcgca gatgcgcgcc cgccatctgc gagttggtgt
     1081 aatagatcag aaccagcgtc gcctcgccgc tgggcgtgtc ataacgcccg gtggttacct
     1141 ctgaacttga gtcgaaatcc acgacgtcgg cgggaacctg cacgcccagc gccgacagtg
     1201 ccagcgggcc ttcggcgtat ttctgcgtat tcgcgatgta tccccgcggc ggcaggaaca
     1261 gcagcaccgg cggcaacaca gcggcgtttc cacttgcacg cggcagcgcc cccgccagct
     1321 cgcgcagctc ggctgccgac atgggcgatt ccctgctgaa caatgcatcc accagaatgt
     1381 gcccacggta aaagagcacg cgcttgccgg tggccgaggc cactgaggcc gcctgatcgc
     1441 cgatttgctc cttggccatc tcgcgctgca aataaaacgt gtacgcgccg aaggcaccgc
     1501 ttgcgtcggc aaagcgcgcg gcgcggatct tcaacgtgcg cccgtcatcg cgggtgtagg
     1561 tggcagaagc gaaatcggag aagcggtact ctttcaggat ggcggcgctc gcggcatccg
     1621 ccgccgcggg atccttgctg gtttgcgccg ggccctgcat ttgccacccg gcgaaggcgg
     1681 gcgggagaac agccggctgg ctgggcgtct ggactgcggg tgttcctaac gccccagcca
     1741 cttccagaaa aaccccaaga aacaccgaaa taccggtaac acggcgaaaa ctcattggta
     1801 acctgacgaa caaacttccc cttaattaga ccacagaccc ggctgaaaag tttcctcgca
     1861 actgcctagt ctgattggga gttcgcaagc ctgattaccc tcgtgcgcgc ctaccaccta
     1921 tcggggccat tctcaaggtc tccgttccgc ggcacactag ctgtgggtga acaatgcgaa
     1981 ccacgagtgt ggaaactaac cagcggcgcg gcaatcgcac caaggccgtc atcccggtac
     2041 gagtgaaagg ccgcgacagt tcgggccagg attttgagga gctggcgcat acgctggacg
     2101 tcaccccggc aggcgtgcgc ctgggctcgg tccgtcgcga actgaatctg ctcgacgaga
     2161 tcaccctttt ctatcgccag cgcaaaatgc aatttcgcgt ggtttggacc aaaaaattaa
     2221 aaggcacgtc ggagtttcag attggtttgg aagctgtgac tcaggaccgc gaggcctggg
     2281 gcatgagttt ccccgaattt aaatccgagg tcctttcgcg gccggctgtc gcacaggcct
     2341 cgggcacggt ataggacgaa ggagaaatgc ttatgggcta tttgccccgc aatctttggt
     2401 tggatgaatc cggtcaggac atcgctgagt attcggtcat gctggctgtg attcttgtga
     2461 tcgtcatcgg tacggtgcgg ctgattggat caaatgccaa caacgtcttc tcggaagtcg
     2521 gcagcgccat ccagtaaagg gctgcctcca cgcgcatggg tgccccatcc taagcgcgct
     2581 ttctgcgctt agggtgggga ctttgaattt ccggatcgat cctcactgca cgctggcctt
     2641 ctccatcttg ctcgccacct tcacaccgct taatccattc acatacttcg cgattccccg
     2701 atagagcgag tcggcgatgc gctgccggta ctcgctggtc tccagccgcc gctcatcccc
     2761 ggggttgctg acgaacgaga tctccgccaa aatcgacggc atattcgccc cgatcagcac
     2821 gatgaatggg gctttcttga ccccgcgatt tctgagtccc ggactcttgg acgccagccc
     2881 gccgtgcagc gactcctgca catcgccggc gaattcccgc gactcttcaa tcttttcctt
     2941 gagcgcgatc tttttcacca ggtcctgcag ttcgtagatc gatttttccg aaaccgcgtt
     3001 ctcgcgcgcc gcaacctcca gggcctccgg cgaggacgtg aaattcaggt agtaggtctc
     3061 gacgccgcgc gccgtctcat cttggctgga gttggcatgg atcgagatga atagatcggc
     3121 gcgctgctgg ttggcaatcg ccgtgcgcgt ttccagcggg atgaacgtgt cgtccttgcg
     3181 tgtgtagacg acttccgcgc ccagccgtgt ctccagcaac ctgcccagct tgcggccgac
     3241 gtcgagcaca agatcctttt cttcgaggcc gttgggcccg atcgttcccg tgtcgtggcc
     3301 gccgtgtccg ggatcgatca cgatcttgcc gatcttcagt ccgagcgccc gaatcagtga
     3361 ccggtcaccc cgggccgtcg gtctggcttc gtgaacgtcg agatcgcggg ggctgtggcc
     3421 cgaggccagc agtgaagctg ttttgccgtt tgccggatct gtgactgcgt cattcgctgc
     3481 cggcttcttt tcgccacccg gctcagtgtc agatgcatca tcgttcttcg agggcttagc
     3541 agccgaggcc gtcgcagtcg ccgtcgaagg cttgtgcagg gaatgcgatt tgcgcttgga
     3601 gtgaacggcc gacacatcgt catcgtcggc ttccacgatc accttcttga ccggctgtgc
     3661 tttcgcttgg aggctggtct cggcgggcgg gcaggttgaa gaattcgccg gattttgggc
     3721 agcgcagcgc ttcagcgctg cgtcaagggt cacttttgtc gaatccgctt cagccgctga
     3781 gggcgcagct tctccctttt ccggagccgt ttgcgccgat gcctcggatg tttccatctt
     3841 tgcgcctact ctcgctgccg cttccacatc ggccgtcgta cttcccgccg catccttcgc
     3901 cgactttccg tgcccgttct tgccgtgaat gtccttgccg tgaatatcaa tgatcaggcg
     3961 gtaaggattg ggcagcagaa acgcgtcata gtcggagaga tcatcaactt caagcaccac
     4021 gcgcgtgcgc cccggctgaa actgcgccac gcgaatcttc ttcaggaagc cgtcgtccac
     4081 gtcgaagctc ttccccacca gcgtcgaggc tagcttggtg tcgcgcaggt caaagaagat
     4141 tcgatccggg cccgagatgc gctgcgaggc gaacttgacg tcgctttcca cgtcaatcgc
     4201 gacacgcgtg tagtcgggtg tcgaccagtg ccggattcca gtcacccgcg gaagtctgcg
     4261 cgaatgccga tctgcggtct cctgcgggct ggcatcggag cgagaatccg agtccttgcc
     4321 cgttgcatcg tttgcgggag agtcctttgc cgtatggttc ttccccgcgt ctgagccgga
     4381 cttcgctttc ttcgcctccg tggccttctc cgcctcggag tcgcggtcca gttcggcaat
     4441 ggcctgctgt gcgtcttcga ccagccggtt gtgcggataa cgatgcagaa attcctggaa
     4501 cgtggctcgg gcctggtcgg gatcgtcgag atcatccttg tagatctcgc caatcgtgaa
     4561 cagtgcgtcg caacgatatt tgctgcccgg gtactcgcgg cgcaaaaact tatactgaga
     4621 aatcgcctcg ttcaggattt tgtcatcgtc aaaccgtcgc cccatttcca ccagcgtttc
     4681 ggctaccgcc accacgctgg gatcggcctt cgtcgaagtc ggcgcgccgt agtagacgtt
     4741 gcgataggcg ttaaggaccc gctgatagtc gcgccgcgtg cggtctgccg gaggccgtcc
     4801 gttcagcgcc tcccgcatgc gctcggcggc agcaaagcgg tcccgcgccc acgattccga
     4861 gtggtgcggg cgcgcgtacg ccggcaccgc gggcagcgcc atcagcaaca gcagcgctcc
     4921 ccagcgcgcc cggcgctcgc tggatctcgt cttcaaatcg ggcttacctg ggtcgaccgc
     4981 ttccgtacct gagagacttc tacctgggtg ccccatttct gggtgcccca tttctcgcgc
     5041 gttcttggcg cgagaagtgg ggattttcca cccccgtttt ccctaaaaca gatgccgcaa
     5101 acctactcag tgttgctgat gtacaacacg ccccgcgcat cccgttgcac cgttaccggg
     5161 tcgtgcgagg cgccggacga actgaaatcg aacccgccgt agtacaggtt cgtaatccgc
     5221 cgcacgtagt tctgggtctc ggcgaagcgg ggaatgcccg cgctgcgcgc caccgcaccc
     5281 gaacccgcgt tgtaggcggc cagggtcagt ttcacgtcgc cgccgtagtt ttcgagcaac
     5341 tgcttcagat gccgcactcc ggcatccacg ttctgctcag gatcaaaggg attcttgacg
     5401 ttcaagcttc gtgccgtcgc tggcatcagt tgcatcagcc ccatcgcgcc cttgcgcgac
     5461 accgcattgg agttgaaatt cgactcaacc ttgatcaccg cgcgcaccag gttgggatcc
     5521 acgttgtgcc gcgctgccgc catcacgata gaagcgtcaa cctcttccgg cgaagcctga
     5581 tggccatgag agttcgctgc aataatgcgc gcgttcgccg atttcgctga ctcgcgcaca
     5641 aagtactggc tcacttccgc cgccgccga
//
LOCUS       DASPUL010000391         2509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_70091, whole genome shotgun sequence.
ACCESSION   DASPUL010000391 DASPUL010000000
VERSION     DASPUL010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70091"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="VEJ00_09565"
     CDS             complement(<1..632)
                     /locus_tag="VEJ00_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HXX01448.1"
                     /translation="MATAPASDVMVPQPYLVREVSKETSDTFTLRLAPEGANGSSFQP
                     GQFSMLWVFGVGELPISISGDPNVHDQLVYTVRSVGQATNALVNQSVGAGVGVRGPFG
                     RGWPMEVARGRDVVVVAGGIGLAPLRGVIYEVLHHREDYGRLVILYGARSPRDLLYRK
                     ELAAWARQRETQVLVTVDYGGMSWRGHVGVVTTLFKYARLKPANSVAMVCG"
     gene            complement(640..1098)
                     /locus_tag="VEJ00_09570"
     CDS             complement(640..1098)
                     /locus_tag="VEJ00_09570"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXX01449.1"
                     /translation="METLERIIAEHPFCAGLDSSFTSLIVGCASNVRFTKGDYIFKEG
                     DPADTFYLVREGKVAVEIAAPQHRPIIIATLAEGEILGWSWILPPYQWKHDARATENV
                     RAIALDGKCLRAKCEENHDFGYEVLKRFARIIEQRLESTRLQLLDVYAAR"
     gene            complement(1085..2272)
                     /locus_tag="VEJ00_09575"
     CDS             complement(1085..2272)
                     /locus_tag="VEJ00_09575"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXX01450.1"
                     /translation="MTAESLLATRESSWSEAVSQKAHAILASGDLAKLIEALVRKGYE
                     VVGPTVRDGAIVYDQVESVGDLPAGWTDEQAPGHYRLKRRDDDALFGYVVGPQSWKKY
                     LHPAEVRLWSAERQNGTFRILNNETKPKTPRAFLGVRACELAAIGVQDRVLLEDRYRD
                     AIYGARRSGTFLIAVQCTQSAETCFCASMGTGPGVSADSPADLVLTELLGPGGHRFLV
                     KASSERGAEVLAELQTTPAQDSDLLEAEEGVKNAAKQSRSIDNNGIKELLYQNFEHPR
                     WDNVASRCLTCANCTMVCPTCFCTNVEDTSDVTGDHAERWRRWDSCFTQSFSYIHGGS
                     VRTSTKARYRQWMTHKLASWIDQFGSSGCVGCGRCITWCPVGIDITEEVRALRDGGRH
                     GNP"
BASE COUNT          477 a          827 c          730 g          475 t
ORIGIN      
        1 ccgcacacca tcgccaccga gttcgccggt ttcaaccgcg cgtacttgaa cagggtcgtg
       61 accacgccca cgtgcccgcg ccagctcatg ccgccgtagt ccacggtaac cagcacctgg
      121 gtttcgcgtt gacgcgccca ggcggcgagt tctttgcggt acaagaggtc tcgcggactg
      181 cgcgccccat agagaatcac cagccggcca tagtcctcgc gatgatgcag cacctcgtag
      241 atcaccccgc gcagcggcgc cagcccgatg ccgccggcca cgacgaccac atcccttccg
      301 cgggcgacct ccatcggcca gcccctgccg aaggggccgc gaacgcccac gcccgctccg
      361 acgctctggt tgacgagtgc attggttgcc tggcctaccg agcgcaccgt gtacaccaac
      421 tgatcgtgaa cgttggggtc gccgctgatc gagatgggca gttcgccgac gccaaacacc
      481 cacagcatgc tgaattgtcc cggctggaaa gaactgccat tcgctccctc gggggcgagc
      541 cgcaaagtga aggtatcgga ggtctctttc gacacctcgc gcaccaggta aggctgcggg
      601 accatcacgt cgctagcggg cgccgtcgcc atcacaatgt cacctcgcgg catacacgtc
      661 gagcagctgg aggcgcgtgg actccaatcg ctgttcgatg attcgcgcga accgcttcag
      721 gacttcgtag ccaaagtcgt ggttctcctc gcatttcgct cgcaggcact tgccgtccag
      781 cgcgatggct cgcacgttct cggtggcgcg agcatcgtgt ttccactggt agggcggcaa
      841 tatccaggac cagcccagga tttcgccctc agccaaagtc gcaatgatga ttggcctgtg
      901 ctggggcgcc gcgatttcca ccgcgacctt gccctcccgc accaggtaaa acgtgtccgc
      961 gggatcgcct tccttgaaga tgtagtcgcc cttggtgaag cgcacgttgg aagcgcagcc
     1021 tacgatcaaa ctggtgaagc tgctgtccag gccggcacag aacgggtgct ccgcaatgat
     1081 tcgctcaagg gtttccatgt ctgcctccgt cgcgcagggc gcggacttcc tcagtaatgt
     1141 caatccccac cgggcaccag gtaatgcaac gcccgcaccc cacacatccg gaactgccga
     1201 actggtcgat ccacgaggcc agcttgtggg tcatccactg ccggtatcgt gcttttgtcg
     1261 aagtgcgaac gctgccgccg tgaatgtagg agaagctctg ggtgaagcac gaatcccagc
     1321 gccgccagcg ctccgcgtgg tcgccggtca cgtcgctcgt gtcctcgacg ttggtgcaga
     1381 agcaggtggg acaaaccatc gtgcagttgg cgcaggtcag gcagcgggag gcaacgttat
     1441 cccagcgggg atgctcgaag ttctgataca gcagttcctt gatgccgttg ttgtcgatgc
     1501 tgcgggactg ttttgccgca ttctttactc cctcctccgc ttcgagaagg tcggaatcct
     1561 gggcgggtgt ggtctgtagt tcggccagca cttccgcgcc tcgttcacta ctcgccttaa
     1621 ccagaaaacg atgcccgccc ggccccaaca actcggtgag gaccagatcc gccgggctat
     1681 cggccgagac ccctggtccg gttcccatcg atgcgcagaa gcaggtttcc gccgactgcg
     1741 tacactgcac cgcaatcagg aacgtacctg agcggcgtgc tccgtaaatg gcatcgcgat
     1801 atcgatcctc gagaagaacg cggtcctgca cgccgatggc agccagctcg caggctcgca
     1861 cgcccagaaa cgcccggggc gttttgggct tcgtttcatt gttgaggatg cggaaagttc
     1921 cgttttgccg ttcggcggac cagagacgta cttcagccgg atgcagatac ttcttccagc
     1981 tctgcggccc gacgacgtag ccaaacagag cgtcatcgtc gcgccgcttc aggcggtaat
     2041 gtcccggagc ttgctcgtct gtccagccgg cgggcaaatc cccgaccgac tcaacctgat
     2101 cgtagacgat ggccccgtcc cgcaccgtcg gcccgacgac ctcatagccc ttccggacca
     2161 gcgcctcaat cagcttcgcc agatcgccgg acgccaggat ggcatgcgct ttttgtgaca
     2221 ccgcctcact ccacgatgat tccctggttg ccagcaggct ctcggcggtc acaacacctc
     2281 cttgcctatc gaaaccaacc aggaaatcgt tgctgcgtcg gttcttacga cctcaacacg
     2341 cttggaggga tcaaaaccag cgtagcacag cagcgggacc agcgttgtca tgctgcaatc
     2401 ggagagacgt acaaagagga cttgccgtac cacaatagga atgctttcac gccgcccgaa
     2461 gatggactgt gacctgcatc acggacggct gtgactgagg aggcggggg
//
LOCUS       DASPUL010000392         3698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1847361, whole genome shotgun sequence.
ACCESSION   DASPUL010000392 DASPUL010000000
VERSION     DASPUL010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847361"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VEJ00_09580"
     CDS             <1..607
                     /locus_tag="VEJ00_09580"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXX01451.1"
                     /translation="AADLFALLAKNQTWQCPTLVWERGGNLIDLSDFAHDPRAKYVPA
                     SWKEVTWKRFTEEVVHEFNVDDLATRRGFVDKELEVVNAMHHAGVPFLAGTDTPPGVY
                     IFPGFSLHEELQRFVAAGFTPLEALQTATLNPAKFLGKEDQLGTIDKGKLADMVLLDA
                     NPLDDIRNTQKIAAVVVSGRYLSRADLDKMLAGVEEAAKGK"
     gene            771..1112
                     /locus_tag="VEJ00_09585"
     CDS             771..1112
                     /locus_tag="VEJ00_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXX01452.1"
                     /translation="MLRDRRRYCVYIMGSLSGTLYVGFSGNLHKRVFQHKFHHYEGFT
                     ARYEVTRLLYWESYDNVHRALARETQLKGWGRAKKVGLIVRQNPHWVDLASEWYPWMK
                     SAEGRDASTAR"
     gene            complement(1188..2102)
                     /locus_tag="VEJ00_09590"
     CDS             complement(1188..2102)
                     /locus_tag="VEJ00_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01453.1"
                     /translation="MNLHPTILLAFLEFSRTIASTCTAGIVLCLIGLLAAKSDVAQAR
                     GLDKVVALGNLFFAAPLAVFGAEHFAAAQGISQFVPKFMPWPLFWTYFVGVALIAASL
                     SIATKIQVRWSGLLWGIMMFLFVSMMDLPGTLKDVHNRISWALLCREMSFGAAGWVLA
                     GNAMVGRDGQGNRTLITIGRVVIGIAAIFYGVEHFLHPLNVPGVPLEKFMPEWIPGRI
                     VIGCLTGLILLVCGAGILLPKKTRTAARYLGSWITLLLFTVYLAILIASFLDPGSDVK
                     VEGINYFFDTMLYAGAILALAKRSESLP"
     gene            complement(2745..>3698)
                     /locus_tag="VEJ00_09595"
     CDS             complement(2745..>3698)
                     /locus_tag="VEJ00_09595"
                     /inference="COORDINATES: protein motif:HMM:NF015883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HXX01454.1"
                     /translation="KELGIFPSQQIEFDRLSLTYTLMSKRLLRRLVEEGRVRGWDDPR
                     MPTLGGLRRRGYTPEAIRNFVAAVGVSRTNGIVELAMLEHFVREDLNKRSPRVMAVLR
                     PLKVVIDNYSENQVEEMDAQNNPEDESAGSRKVPFSRVLYIEQDDFREVPPPKYFRLS
                     PGREVRLRYGYFITAKSVVKNEKGEVIEVHCTYDPATRGGNAPDGRKVKSTIHWVSAA
                     HAVDTEVRIYDNLFSKEDPTDVPEGQDFTANLNPNSLEVIAHAKLEPSLANAAAGSRY
                     QFERLGYFCADPDSKPGAPVFNRTVALKDTWAKVEKKQGRS"
BASE COUNT          752 a         1040 c         1155 g          751 t
ORIGIN      
        1 tgccgcggat ctttttgcgc ttctggcaaa gaatcagacg tggcagtgtc cgactttggt
       61 gtgggagcgc ggcggcaacc tgatcgatct ctcggacttc gcccacgacc cgcgcgccaa
      121 gtacgtccct gcgtcatgga aggaggtgac ctggaagcgc ttcaccgagg aggtcgtcca
      181 cgaattcaac gtggatgatc tggccacgcg ccggggcttc gtcgacaagg aactcgaagt
      241 cgtcaacgcc atgcaccatg ccggcgtccc gtttctcgcc ggcaccgata cgcctcccgg
      301 agtttacatc tttcccggct tcagcctgca cgaagagctg cagcgcttcg tcgccgccgg
      361 attcacaccg ctggaggcac tgcagacggc tacgctgaat ccggcgaagt ttctgggcaa
      421 agaagatcag ctggggacga tcgacaaagg aaagctggcc gacatggtcc ttctcgatgc
      481 aaatccgctg gacgatattc gcaacacgca gaagattgca gcggtggtgg tgagcgggcg
      541 ctatttgtcg cgggcggatc tggacaagat gctggcggga gtggaggagg cggcgaaagg
      601 gaagtagaga aacagatttg tcatgctgag cggagcggga gaagcgcgaa gcgattctcc
      661 ggcggagtcg aagcatccct acccaaacac gaattcccga tgtgggaaca cctgcagaat
      721 ttgtgacagt ccgcactgcg tagcgatgca agatccacga tcattcccga gtgcttcgcg
      781 atcgcagacg ctactgcgtg tacatcatgg gcagcctgtc cggcacgttg tacgtcggat
      841 tcagcggcaa cctgcacaaa cgtgttttcc agcacaaatt ccatcactac gagggattca
      901 ccgcccgata cgaggtgact cgtctgctgt actgggaatc ttatgacaac gtccacaggg
      961 cgctcgcgcg cgagacccag cttaaaggat ggggccgggc gaagaaggtt gggttgattg
     1021 tccgccagaa ccctcactgg gttgacctgg cgtcggagtg gtatccgtgg atgaaatctg
     1081 cagagggtag ggatgcttcg actgcgcgat gaggatcgct ttgctctcct catctctccg
     1141 ctcggcatga caaacgtcgc gcacagcgac tatttgcccc cgcgccgtca gggaagactc
     1201 tccgaccgct tcgcgagggc gagaatcgct ccggcataca gcatggtgtc gaaaaagtag
     1261 ttgatccctt cgaccttcac gtcggagcca gggtccagga acgaggcgat caggattgcc
     1321 agatagacgg tgaagagcag cagcgtaatc cacgagccga gataccttgc cgccgtccgc
     1381 gttttctttg gcagcagaat gccggcccca caaaccagca gaatcaagcc tgtcaggcag
     1441 ccgatgacta tacgccccgg gatccattcg ggcatgaatt tttccagcgg aacgcctgga
     1501 acgttgaggg gatgaagaaa gtgttcgacg ccgtagaaga ttgcagcgat gccgatgacg
     1561 actcggccta tggtgatgag cgtcctgttt ccttgcccgt cgcgtccgac catcgcgttc
     1621 cccgcaagaa cccagcccgc ggcgccgaac gacatctcgc ggcacagaag ggcccaggaa
     1681 attcggttgt gcacatcctt cagcgtgccg gggaggtcca tcatcgacac gaacagaaac
     1741 atcatgattc cccacagcag gcccgaccag cgcacctgaa ttttcgtggc gatgctcagg
     1801 gatgcagcga tcaacgcgac gccgacgaaa taggtccaga acagaggcca tggcatgaac
     1861 ttggggacga actggctgat gccttgagcg gcggcaaaat gctcggcgcc aaagacggcc
     1921 agtggcgcgg cgaagaaaag attgccgagg gcgacaacct tgtcgaggcc gcgggcctgg
     1981 gcgacatcgc ttttcgcggc cagcaggccg atcaggcaga ggacgatgcc ggcggtgcag
     2041 gttgaggcga tggtgcggga gaattcgagg aaggcgagga gaatggttgg gtgcaggttc
     2101 atggcaagcc gatcaggggc agcatcgctt tcgcgaaatc atactgcgaa tttggacggt
     2161 gctggagcga gaaaggttag ggactcatgg tttccttctt ggtggcatac ccctgattgc
     2221 ttgtcatcct gagcgaagcg agggatctgt gcacttggcc ggggattgca taagtccctt
     2281 cgagtccgct cagggcaggc tctttggcgc aaaaatcgcg cctcaggatg acaaagctga
     2341 agtttttgct gattgtcgcc gatagcgatt tcagagctgg acaggcagag atgctgaccc
     2401 cgagtggaag atgcgctcgg ggtttcattt ttggcgtgga ggccgaccta gagaagttgc
     2461 ttcttcagca cgacgaattc tgttatgccc tcagtggatt caagcgtctt gatcctgact
     2521 acctctttgc cggtcgcgtc cgtccacgca tccagcacct ggcagcgaac ggtagcaggt
     2581 atcggatatg aatcctgttc tgacagtagc ctgtcgacga atatgagtac ctttgtcgac
     2641 gatgacatgg gttcgattgg ccgcatccac gaactcacat tcgacaatcc cgggttgagg
     2701 gtcatcgcct aggtacttcg ttattgtcac ggggagctcc aacttcagct cctgccctgc
     2761 ttcttctcga ccttggccca ggtgtctttc agcgcgactg tgcggttgaa gactggtgcg
     2821 ccgggcttgg agtcgggatc ggcgcagaag tatcccaggc gctcgaactg gtagcggctg
     2881 cccgcggcag cgttggccaa agacggttcc agcttcgcgt gcgcgatcac ttccagcgaa
     2941 ttcggattca ggttcgcggt gaaatcctgc ccctcaggaa cgtctgtggg atcttccttg
     3001 ctgaacaggt tgtcgtagat gcgcacctcg gtgtcgacgg cgtgcgcggc cgagacccaa
     3061 tggatggtcg actttacttt ccgaccgtct ggagcgttac cgccgcgcgt ggcgggatcg
     3121 taggtgcaat gcacctcgat gacttcgccc ttttcgttct tcaccacgct cttcgctgtg
     3181 atgaagtagc cgtagcgcag gcgcacttcg cgcccgggcg acaggcggaa atattttggc
     3241 ggaggcactt cgcggaagtc gtcctgctcg atgtagagca cgcgcgagaa cggcaccttg
     3301 cgcgagccag cgctttcgtc ttcgggattg ttctgcgcgt ccatctcttc gacctgattc
     3361 tcagagtagt tgtcgatcac gaccttgagc gggcgcagca cggccatgac gcgcggggag
     3421 cgcttgttca agtcttcgcg cacgaagtgt tcgagcatgg ccagttcgac gatgccgttg
     3481 gtgcgcgata ccccgaccgc tgcgacgaag ttgcgaatcg cctcgggcgt gtagccgcgg
     3541 cggcgcaggc cgccgagcgt cggcatgcgc ggatcgtccc agccgcgcac gcggccttct
     3601 tcgaccaggc ggcgcagcag gcgcttcgac atcagcgtat aggtcaggct caggcggtcg
     3661 aattcaatct gctgcgacgg gaagatgccc agctcttt
//
LOCUS       DASPUL010000393         4223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1847381, whole genome shotgun sequence.
ACCESSION   DASPUL010000393 DASPUL010000000
VERSION     DASPUL010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847381"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VEJ00_09600"
     CDS             <1..678
                     /locus_tag="VEJ00_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXX01455.1"
                     /translation="LVSATALRDEEGNFQMSSSVVYDLTYRKRADSRFRAVLEGAPDA
                     LLMCNRKGEVVLTNSQIEKIFGHSPEELRGRSFTVLLPERLRALHSDHVEGFFCNPAQ
                     RPMGESKRLSGLRKDGTEIPVEISLSPLQTDEGLVALAAIRDVSERRRNEEEREKLIE
                     ELKQAVENVKLLSGLLSICAACKRIRDEKGHWESLEVYIRDRSSANFTHGICPDCVRR
                     LYPKSDD"
     gene            complement(859..3588)
                     /locus_tag="VEJ00_09605"
     CDS             complement(859..3588)
                     /locus_tag="VEJ00_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX01456.1"
                     /translation="MGEKNGQAGNANPGALETQPASTPSPASDPNVTLSSSAAAFSAA
                     ERAPQLKSIGPYHLIRRLGEGGMGQVWLAEQTAPIQRQVALKLIKVGRYDDSVLQRFY
                     AERQSLAIMDHPAIAKVFDAGATPDGQPYFVMEYVPGVPITDYCDRKRLRIRERLELF
                     MKVCEGVQHAHQKAIMHRDLKPANILVVEVDGKPVPRIIDFGLAKPASPQIDGQTMFT
                     QAGGWVGTPGYMSPEQADPGVLDVDTRTDVYSLGAVLYVLLTGFLPFENRDWRKQRFD
                     EFLRHLREDDPPRPSTKVSTEKGSSKSAAEARGTEPRHLAGLLHGDLDWITMKALEKD
                     RNRRYGTPSELATDIEHYLRHEPVVARPASTGYRLRKYMRRHRASVAVTAGLVLLLAG
                     FAVTQAVQVRRITRERDRANRIAEFMSNVFRVNNPSQARGNTITARELLDKAAAQIPT
                     ALAKDPELQADMMDQMGRVYEHLGLTSRAEDLLRPGVEIRRRVLGSNHPETLLSINNL
                     ANALSDEGKLTDADVLYKEVIDRSRRVLGPAHHTTTMAMNNYALNLQDEGHSTEAEKM
                     LRDVVEAHSRALGSEHPETLTAISNLANMLRMNGHFAESEKLNRQVLAARRRILGADD
                     PDTLRSATNLALVLTDEQGHDLEAEQLLRPTLETQKRIVGPEHPDTIETMNSLATVCG
                     RQGKLAEEETLLQQTIAIERRTLGPDSSKVFVSMNNLAALYEEEKKLPEAERIFRELV
                     EVQSRSRGPQGYYTLVATTNLAAALQDEKRFGEAEKLGHSALEIERRNFGEDDPLTIE
                     QLRNLAMLEQDLHHDDEAEKLLNEAVEHQTKVYGVDNPQTAKLNYALGAFYATHGNHD
                     KAFSFLRQAVEHGLDATGLGGIDKDPDLKALHSDPRFAELLSSARQRASPKLD"
BASE COUNT          801 a         1176 c         1343 g          903 t
ORIGIN      
        1 ctggtgagcg cgaccgctct gcgcgatgaa gaaggcaact ttcagatgag cagctcggtt
       61 gtgtacgacc tcacctaccg caagcgggcg gacagccgat tccgcgctgt gcttgagggt
      121 gcgccagacg cattgctgat gtgcaatcgc aaaggcgagg ttgttctgac caattctcag
      181 atcgagaaga tctttggcca cagcccggag gagttgcggg ggcgcagctt caccgtgctg
      241 ctgcctgagc gactgcgcgc tctccacagc gatcacgtag agggattttt ctgtaatcca
      301 gcgcaacggc cgatggggga aagcaagcgt ttgagcggac tacggaaaga cggaacggag
      361 atccctgtgg agatcagcct gagcccgctt caaacggatg aaggactggt ggcattggcg
      421 gccattcggg acgtttccga gcgccggcga aacgaggagg aacgcgagaa gctgatcgag
      481 gaactcaaac aggctgtcga aaacgtgaag ctgttgagtg gcctgttatc tatctgcgca
      541 gcgtgcaaga gaattcgtga cgagaagggc cattgggagt ctcttgaggt ctacatccgc
      601 gaccggtctt ccgctaactt tacccacggc atctgtcccg actgtgttcg caggctctac
      661 ccgaaatccg acgactagga cactctagtc cgcgagcggc aggggtcaga ggtcagggat
      721 caggggctat agattgagga ccagttatta gcacgcggtt ttagaggttt catcgcgcct
      781 tcgtcttatt cgaaaccgtt tttccagcga ttgaagaaga ccaaccccac ggcttaacgg
      841 caagctggga attgccgtct agtcgagttt aggagaggcg cgctggcggg ccgatgagag
      901 aagctccgcg aagcgtggat cggaatggag ggccttgagg tcggggtcct tgtcgatgcc
      961 gccaaggcct gtggcgtcca ggccgtgctc aacggcctgc cgcaggaatg agaaggcctt
     1021 gtcatggttg ccgtgagtgg cataaaaggc gcccagcgcg taattgagct tcgcggtctg
     1081 tgggttatca actccgtaca ctttcgtttg gtgctcaaca gcttcattaa ggagtttctc
     1141 agcctcgtcg tcgtgatgga gatcctgctc cagcatggca agattgcgca gctgttcgat
     1201 ggtcaaagga tcatcttccc caaagttacg ccgctcgatt tccagcgcgg aatggccgag
     1261 tttctcggct tccccgaatc gcttctcgtc ctgcaatgcc gccgccaggt tggtggtagc
     1321 caccagcgtg tagtagccct gcgggccgcg ggaacgagac tggacttcaa caagttccct
     1381 gaagatgcgc tcggcttccg gcagcttctt ctcttcttcg tagagcgcag caaggttgtt
     1441 catgctgacg aacactttcg aactgtcggg accgagagtt cggcgttcaa ttgcgatcgt
     1501 ctgctgcaat aaagtctctt cttctgccaa cttgccttgt cgaccgcaga ccgtggcgag
     1561 actgttcatc gtttcgattg tgtcgggatg ttcggggccg acaattcgct tttgtgtttc
     1621 cagggtcggc cgaaggagtt gctccgcctc taagtcgtga ccctgctcgt cggttaacac
     1681 caaggcaaga tttgtagcag acctcagggt atctggatcg tcagcaccga gaatgcggcg
     1741 gcgagcggcg agaacctgac ggttgagttt ttcggactcg gcaaaatggc cgttcatgcg
     1801 cagcatgttg gccaggttgc tgatagccgt cagggtctcc ggatgctcag agcccagcgc
     1861 ccgactatga gcctctacaa cgtcgcgcag cattttctcg gcttcggtac tatggccctc
     1921 gtcctgaagg ttgagggcat agttgttcat cgccatggtc gtggtgtgat gtgcgggacc
     1981 gagcacgcgg cgagaccggt caatgacttc tttgtacagc acatccgcgt ccgtgagctt
     2041 gccctcatcg gacagcgcat tcgcgagatt gttgatggaa agcagcgtct cggggtgatt
     2101 cgaaccgagg acgcggcgcc gtatctcgac gccaggcctg aggagatcct ctgccctgga
     2161 agtgagccca agatgctcat agacccgccc catctgatcc atcatgtcgg cttgtaactc
     2221 ggggtctttg gcaagagcag tggggatctg cgccgcagct ttatcgagga gctcgcgtgc
     2281 ggtgatggta ttaccgcggg cctgactggg gttattgacg cggaagacgt tgctcatgaa
     2341 ctccgcaatg cggttggcgc ggtcgcgctc gcgggtgatg cggcgcactt gtaccgcctg
     2401 cgttacggca aaaccggcca gcagcagaac caggccggcc gtcaccgcta cgctcgcgcg
     2461 atgccggcgc atgtatttgc gtaaccggta gcccgtgctg gctggacgcg ctaccacggg
     2521 ctcatggcgg agatagtgct cgatgtcggt agcgagctcg gagggcgttc cgtagcgccg
     2581 gttgcggtcc ttttccagcg ccttcatggt gatccagtcc agatcgccgt gcagcaggcc
     2641 ggccaggtgc ctgggctcgg tgccgcgcgc ttccgcagcc gatttggaag atcccttttc
     2701 tgtgctgacc ttcgtgctgg ggcgtggcgg atcgtcttcg cgcagatgcc gcagaaattc
     2761 gtcgaagcgc tgcttgcgcc agtccctgtt ctcgaaggga agaaagccgg tcagcagtac
     2821 gtagagcacg gcaccaagcg aatacacgtc ggtgcgggta tccacgtcga ggacgccggg
     2881 atcggcctgt tccgggctca tgtagccggg cgtgcccacc cagccgcccg cttgcgtgaa
     2941 catggtctgg ccgtcgattt gcggactcgc cggcttggcc agcccgaagt caatgatgcg
     3001 cggcacgggt ttgccgtcga cttccaccac caggatgttg gccggcttga ggtcgcggtg
     3061 catgatggcc ttctgatgtg cgtgctgcac gccttcgcag accttcatga acagttccag
     3121 acgctcccga atcctgagcc gtttgcgatc gcagtaatcg gtgatgggca ctccgggcac
     3181 gtactccatc acaaaatagg gctggccgtc gggcgtggcg ccggcgtcga acaccttggc
     3241 aatcgccgga tgatccatga tggccagcga ctggcgctcg gcgtagaaac gctgcaacac
     3301 agagtcgtcg tagcggccga ccttgatgag cttcagggca acctggcgct gaatgggagc
     3361 ggtttgctcc gcgagccaga cctgtcccat gcccccttcc ccgagtctgc ggatcaggtg
     3421 gtatgggccg atggatttga gttgcggggc acgttctgcc gctgagaacg cggcggcaga
     3481 gctgctcagg gtgacgttcg gatcggaggc cggcgacggt gtactggcgg gctgcgtttc
     3541 gagcgcgccg gggttggcgt tccctgcttg cccattcttt tcgcccatgg ccgccccgcg
     3601 gaagttggga gcagtgtaag cagggcggaa atggctgtca atttgaatcg ggagggcggg
     3661 acttaccggt cgaagtcggt gtcttgcagg cggtcatgag cctgtcaacc accgcaatgt
     3721 gaaatttctg tgacaagctg tccggccagc cacaacaaca tctcttgaat gttgggcacg
     3781 tttgcaacat cgcccacact ggctgcagaa tctagtcaga cagtttgcgc cacctcgtcc
     3841 tgagatctgc aaggttcatc caacctggga gttttcacct tggggttcag cgatccggaa
     3901 attcaaaaga aggtgttcgg accgagatgg cagcacgagg tcgaacggca aagctggccg
     3961 ttgtggaaac ggctgctgca tcgtctgcaa cgatgtccca tatgcaaacc acggcatgaa
     4021 acgtccgggg gcgaaacaaa agatcgaaag actcagtaga atgccggcta ggccagcagc
     4081 ggcagttgcg ggccggcggg cgcgtcggag tcgtcggcgg ggtcggcgaa ctttccgagc
     4141 tccggatact tttgaattag cgtcggcggg gcgttcaccc tcttcccgct gatcaacgcc
     4201 tttagctcga gcgcattcac tcc
//
LOCUS       DASPUL010000394         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1717679, whole genome shotgun sequence.
ACCESSION   DASPUL010000394 DASPUL010000000
VERSION     DASPUL010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1717679"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..728
                     /locus_tag="VEJ00_09610"
     CDS             3..728
                     /locus_tag="VEJ00_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXX01457.1"
                     /translation="MRFNFLQPPFNNQKMREAVLHAVNQRDYVLAIAGDEKHGRPCYS
                     FFTCGTPLSSEIGAESLKGKRDFDRAKKLIQEAGYKGEKIVILSATDVPFVHSQALIT
                     TDLLRKLGLNVELQAGDWGTLTTRRTSKEPVDKGGWSIFHSYAAGAQLISPAGGPLRA
                     SGENAWFGWPTDRKLEELRDAWFNATNAASAKAAADAVQVQAFKFLPFIPTAQFVIPT
                     AYRSNLSGVIVAPMIFLWNVEKK"
     gene            868..1809
                     /locus_tag="VEJ00_09615"
     CDS             868..1809
                     /locus_tag="VEJ00_09615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX01458.1"
                     /translation="MITYILRRLGATLIVMAVVALVVFSLLFLTPGDPAAVIAGDIAT
                     DDDIRQIRAKLGLDRPFLVQFGGWIWRLLHGDLGTSIFTNLPVTTLIGQRVQPTLSLT
                     LCTLVVAVSIAVPLGVIAAAKAGGWADRLVMGFSVLGFSVPVFVLAYLLIMLFAVQLH
                     WLPVQGYQPLSDGLWPWLSHLILPSIALGTVYMALIARITRAAMLDVLAQDYIRTANA
                     KGLAPRTVLSRHALKNAGVPIVTVVGLGVALLISGAIVTETVFAIPGIGRLTVDAILR
                     RDYPIIQGVILLFSAVYVLINLLVDLSYRLFDPRIRY"
     gene            1950..>3136
                     /locus_tag="VEJ00_09620"
     CDS             1950..>3136
                     /locus_tag="VEJ00_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXX01459.1"
                     /translation="MRRILLALLVFAALFCEHATFALAQSEQHPLAPPDFSSPRASLM
                     SFKAVMNAVYQRWKTEGRSYQNRAQLAAAGRLGPMAFDLDDVAPSVRINVGRESAVFI
                     KEVLDRIGLPPWDEIPDQATLAAKPGLTKWTIPYTEITLVRIKDGLREGQWVFSSETH
                     HRAAEFYERVRQLPYLPGSTEGLYELFVSEPGWLIPQSFVRALPGWMKARWGDQVVWQ
                     WCALVLTLLVTAALMIVIYRIGQRWSRGQHGASYYLAVVFPLFAFILPQLATAFLSNH
                     VFITGQLLANIDFALDLVSLAALVVLIMWVTSRVANALMALPWFKPGQLNAQLAQLIV
                     RALGVAAAVIAVFEGGKYVGVPVATLIAGISVSGLTVALAAQDTLKHLFGSLMILLDR
                     PFQV"
BASE COUNT          527 a          983 c          971 g          655 t
ORIGIN      
        1 tgctgcgttt caatttcctg caaccgccgt tcaacaatca aaagatgcgc gaggcggtgc
       61 tccacgccgt caatcaacgc gactacgtgc tcgccatcgc aggcgatgag aagcacggac
      121 gaccctgcta ttcgttcttc acctgcggga cgccgctgtc gtctgagatc ggcgccgagt
      181 cgctgaaagg taagcgcgac ttcgatcgtg cgaagaagct tatccaggaa gcgggttaca
      241 agggtgagaa gattgtgatc ctgtcggcta ccgacgtgcc tttcgtgcac tcacaggcgc
      301 tgatcacaac tgatctgctg cgcaagctcg gcctgaacgt agagctgcag gcgggggact
      361 ggggcacgct cactacgcgc cgcacatcga aagagccggt cgacaagggc ggctggtcga
      421 tctttcactc gtatgcggcc ggcgcgcagc tcatctcgcc ggccggcggg ccgctccgcg
      481 cctccggtga aaatgcatgg ttcggctggc cgaccgacag gaagctcgag gagctgcgcg
      541 acgcatggtt caacgcgacc aacgcagcct ccgcaaaagc ggccgccgac gcagtgcagg
      601 ttcaggcttt caagttcctg ccctttattc caacggcgca attcgtgatc cccacagcgt
      661 accggtcgaa cctgtccggc gtgatcgtcg cgccgatgat ctttctgtgg aacgtggaga
      721 agaagtgaac ggaattccaa ccaccccgcc cactatcgtg ggcacccctc cttggaaagg
      781 aggggagaaa attagtcttc tcccctcctc tcacgaggag gggtcggctg aaagccgggg
      841 gtggtgtggg ttcaaattga gagcattgtg atcacctaca tcctccgccg cctcggcgca
      901 acgctgatcg tcatggcggt ggtggcgctg gtggtgttct ccctgctctt cctcacgccc
      961 ggcgacccgg ccgcggtgat cgcgggcgac atcgctaccg acgacgacat caggcagatc
     1021 cgcgcgaagc tcgggctgga ccggccgttc ctggtgcagt tcggcggctg gatctggcgg
     1081 ctgctgcacg gggatctcgg gacctccatc ttcaccaatc ttccggtcac gacgctgatc
     1141 ggccagcgcg tccagcccac gctgtcgcta accctctgca cgctggtcgt cgcggtgtcg
     1201 attgcggtgc cgctcggcgt tatcgcagcg gcaaaggcag gcggctgggc ggaccgtctc
     1261 gtcatggggt tctcggtact gggcttctcg gttccggtgt tcgtcctcgc ctatctgctc
     1321 atcatgctgt tcgccgtgca gcttcactgg ctcccagtgc aggggtacca gccgctgagc
     1381 gacggtctgt ggccttggct gtcgcatctg atcctgccca gcatcgcgct cggcacggtc
     1441 tacatggcgc tcatcgcgcg catcacgcgc gccgccatgc tggacgtcct cgcgcaggac
     1501 tacatccgca ccgcaaacgc gaaaggactt gcgccgcgca cggtgctgtc gcgccacgcg
     1561 ctgaagaacg ccggcgtgcc gatcgtcacg gtggtcgggc tcggcgtcgc gctgctcatc
     1621 agcggagcca tcgtcaccga aacggtattc gcgattccgg gcatcgggcg gctcacggtc
     1681 gacgcaatcc tccgccgcga ctatccgatc atccagggtg tgatcctgct cttctcggcc
     1741 gtgtacgtgc tgatcaatct gctcgtcgat ctgtcctatc ggctgttcga tccgcgcatt
     1801 cgctactgac gcgacgcctt ctggtccgag caggtgcggt ccggccacat cggggtttcc
     1861 cgatagggcg tcgctctgga ttagaatgaa ctgatcgtca tctgcacctg acctcgcgcg
     1921 ttcgccatct caagatgaaa tcgtcatcga tgcgacgaat cctgctggcg ttgcttgtct
     1981 ttgccgccct cttttgcgaa cacgccacct ttgcactggc gcaaagcgag cagcacccac
     2041 tcgcaccacc cgatttctcc agcccgcgtg cctcgctcat gagcttcaag gcggtgatga
     2101 acgccgtcta ccaacgctgg aagaccgaag gccgcagcta ccagaaccgg gctcagcttg
     2161 cggcggcggg gcggttgggg ccgatggcct tcgacctgga tgacgtggca ccgtcggttc
     2221 ggatcaatgt cgggcgcgag agcgcagtgt ttatcaagga ggtgctcgac cgtatcgggc
     2281 taccgccttg ggacgagatc cccgaccagg cgacgcttgc cgctaagccg ggattgacca
     2341 agtggacgat cccatacacc gagatcaccc tggtccgtat taaggatggg ctgcgcgagg
     2401 gtcagtgggt attcagctcg gagacccatc accgggcagc ggagttctac gagcgggtaa
     2461 gacagctgcc ctatctaccg ggctccaccg agggtttgta tgagctgttt gtctccgagc
     2521 cgggctggtt gattccgcag tcatttgttc gcgcgctgcc cggatggatg aaggcgcgtt
     2581 ggggtgatca ggtggtgtgg cagtggtgcg cactcgttct gaccctactc gtcaccgccg
     2641 cactcatgat cgtcatctat cgcatcggcc agcgctggtc ccgaggccag cacggagcga
     2701 gctactatct cgccgtggtg tttcccctct tcgccttcat ccttcctcag ttggcgacgg
     2761 cctttctttc caatcacgtg ttcatcacgg ggcaactttt ggcgaacatc gattttgcgc
     2821 tggacctggt ctccctggct gcgttggtcg tgttgatcat gtgggtgaca agccgggtgg
     2881 cgaacgcgct gatggcattg ccttggttca agccaggaca gctgaacgcg caactggctc
     2941 agttgatcgt gcgggcattg ggtgtggccg ccgccgtaat cgccgtcttc gagggcggaa
     3001 agtacgtggg tgtgcccgtg gcgacactca tcgcgggaat cagcgtgagc ggtttgactg
     3061 tggccctggc cgcgcaggac actctaaaac atctcttcgg cagcctcatg atcctgctgg
     3121 atcggccctt ccaagt
//
LOCUS       DASPUL010000395         3099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1024374, whole genome shotgun sequence.
ACCESSION   DASPUL010000395 DASPUL010000000
VERSION     DASPUL010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024374"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2465
                     /gene="dnaE"
                     /locus_tag="VEJ00_09625"
     CDS             <1..2465
                     /gene="dnaE"
                     /locus_tag="VEJ00_09625"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXX01460.1"
                     /translation="IIQQMKFPGYFLIVWDFIRYAKERDIPVGPGRGSAAGSLVSYAL
                     GITDLDPLQHELLFERFLNPERVSMPDIDIDFCMNRRGEVIDYVTQKYGRENVAQIIT
                     FGTMAAKAAIKDVGRAMDIPYADVDRIAKMVPTTLNITLDEALEESPPLAEAYEKDTQ
                     IRELLDTARKLEGLVRNAGVHAAGVVIAPRPLTELVPLHKTKNDEIVTAFDMVAIEKM
                     GLLKMDFLGLTTLTILTDTLKLIQQTRGQTLTLEQIPLEDHETYEKVFHRGLTSGVFQ
                     FESHGMRDVLRRYQPNSIEDLTALNALYRPGPIQGGMIDDFVDRKHGRKKVEYELPEL
                     KEILEETLGVIVYQEQVMQIANRLAGYSLGEADLLRRAMGKKKPEEMAQQRERFIEGA
                     VQRKYPPKKIEKIFDLMAQFAGYGFNKSHSAAYALLAYHTAYLKTHFPVEFMAALLTS
                     VTGSTDDVVKYINECREMGIAVEAPDINVSDANFTPRGESIRFGLAAVKNVGGNAIES
                     IVAARKKLGRFKSIFEFCEHVDLRLLNKRVLESLVKSGAMDSLGRRAQLVAVLDKAIE
                     QAQKVQRDLESGQHGLFGVFAQDETEDHNHKLPDTPDWDEHTRLTAEKEILGFFITGH
                     PLEKYRDKLADLQALSTGEIAAMKSSTGKDETIVTAGIITNRRVLKSKRGDFYAQATL
                     EDMAGSIDMIVFPEAFKRIGEKVKLEVPVLVRAGVRIEEGANPKVTASEIIPLEEAKV
                     PVPTAIRIRVPLETSAESTVDELHKLFVERKGDAKVLFDVERQGDFMVVMEAEGYNVL
                     PDRAFMARVEELCGRGSVRVIS"
     gene            2579..>3099
                     /locus_tag="VEJ00_09630"
     CDS             2579..>3099
                     /locus_tag="VEJ00_09630"
                     /inference="COORDINATES: protein motif:HMM:NF015228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXX01461.1"
                     /translation="MALTEKAQQELEQIERQIAEMESVGTDEATRRQIAKLQERIETL
                     RRDLNAPSTAWQKTELARHPQRPQTLDYIERVFTDFSEIHGDRGFADDAAIVCGMARF
                     HGDEVLVVGTQKGRDMKQRVYRNFGTPHPEGYRKAIRIMKVAEKFHRPIFTLVDTDGA
                     YPGIHAEERGQAEA"
BASE COUNT          698 a          854 c         1002 g          545 t
ORIGIN      
        1 ggatcatcca gcagatgaag tttcctggat atttcctgat cgtctgggat ttcattcgtt
       61 acgccaagga gcgcgacatt cctgtcggcc cggggcgcgg atcggcagcc ggttcgctgg
      121 tttcctacgc gctcggcatc actgatctcg atccgctcca gcacgaactg ctgttcgagc
      181 gctttctgaa tccggagcgc gtgtcgatgc ccgacatcga catcgatttc tgcatgaacc
      241 gtcggggcga ggtaatcgat tacgtcacgc agaagtacgg gcgcgagaat gtggcgcaga
      301 tcatcacgtt cggcacgatg gcggcgaagg cggcgattaa agacgtgggc cgggcgatgg
      361 acatcccgta cgccgacgtc gaccgcatcg ccaagatggt gccgacgacg ctgaacatca
      421 cgctggacga agcgctggaa gagtcgccac cgctggcaga ggcgtacgag aaagatacgc
      481 agattcgcga gctgctcgac accgcacgca agctggaagg gctcgtccgc aatgcggggg
      541 tgcacgcggc aggagtggtg atcgcgccgc gtccgctgac ggaactcgtg ccgctgcata
      601 agaccaagaa cgatgaaatc gtgaccgcct tcgacatggt cgctatcgag aagatgggcc
      661 tgttgaagat ggactttctc gggctcacca cgctcacgat tctcaccgac acgctgaagc
      721 tgattcagca aacgcgcggg cagaccctta cgctggagca gattccgctg gaagatcacg
      781 agacttacga aaaagtcttc caccgcgggc tgacttccgg cgtattccag tttgaatcgc
      841 acgggatgcg cgacgtgctg cggcgttacc agccgaattc catcgaagac ctcacggcgc
      901 tgaatgcgct ttatcggcct ggaccgattc agggcggaat gatcgacgac ttcgtcgacc
      961 gcaagcatgg gcgaaaaaaa gtcgaatacg aactgcccga gctgaaggag attctcgaag
     1021 aaacgctggg cgtgatcgtg tatcaggaac aagtgatgca gatcgccaac cggctcgctg
     1081 gatactcgct cggtgaggct gaccttttgc gccgcgccat gggcaagaag aagccggagg
     1141 agatggcgca gcagcgcgag cggttcatcg aaggcgcggt gcagaggaag tatccaccga
     1201 agaagatcga aaagattttc gatctcatgg cgcagtttgc gggatatggc ttcaacaagt
     1261 cgcactcggc ggcatatgcg ctgctggcct accacacggc gtatctgaag acccacttcc
     1321 cggtcgagtt tatggcggcg ctgctgacgt cggtgaccgg ttcgaccgac gacgtggtga
     1381 agtacatcaa cgaatgccgc gagatgggaa tcgcggtcga agcaccggac atcaacgtgt
     1441 cggatgcgaa ctttaccccg cgtggtgagt cgatccgctt cggactggcc gcggtgaaga
     1501 atgtcggcgg caacgccatt gaatcgattg tcgcggcgcg caagaagctg gggcggttca
     1561 aatcgatttt cgaattctgc gagcacgtgg acttgcggct cttgaataag cgcgtattgg
     1621 aatcgctggt caagagtggt gccatggatt cgctgggacg gcgcgcgcag ctggtggcgg
     1681 tcctcgacaa ggcgatcgag caagcgcaga aggtacagcg cgacctggag tccggacagc
     1741 acgggctgtt cggcgtgttc gcgcaggacg aaaccgaaga tcacaatcac aagctgccgg
     1801 acacgcccga ctgggacgag cacacgcgtt tgactgccga aaaggaaatt ctgggattct
     1861 tcatcactgg gcatccgctg gagaaatacc gcgacaagct cgccgacctg caggcgctaa
     1921 gcacgggtga aatcgccgcg atgaaaagct cgaccggcaa ggacgagacc atcgtgacgg
     1981 cgggaatcat cactaaccgg cgcgtgctca aatcgaagcg tggggacttc tacgcccagg
     2041 ccacactcga agacatggct ggatcgatcg acatgatcgt cttccccgag gctttcaaac
     2101 gcatcggaga gaaagtcaag ctcgaagttc cggtgctggt gagggcgggc gtgcgcatcg
     2161 aagaaggagc gaacccaaag gttacggcgt cagagattat tccgctggaa gaggccaagg
     2221 tgccagtgcc cacggcgatc cgcattcgcg ttccgctcga gacctcagcc gagtccaccg
     2281 tggacgaact gcacaaactg tttgtcgagc gtaaaggcga cgccaaggtg ttgttcgacg
     2341 tggagcggca gggcgatttc atggtagtga tggaagcgga aggctataat gtcctgccag
     2401 accgcgcttt catggcccgc gtggaagagt tgtgcgggcg gggcagcgtg cgcgtgatca
     2461 gctagcgata gacgccttcg ctgctaaacg cagtggagct gcacgcgaga tccctcggcc
     2521 gctggtgaaa acgcgggcct tcgggatcaa gccatcgaat tgtgacgatg cactgagcat
     2581 ggcactcacg gaaaaagcgc agcaggaact ggagcagatc gagcgccaga tcgcggaaat
     2641 ggaatcggtg gggaccgatg aggccactcg gcggcagatc gccaagctgc aggagcgcat
     2701 tgagactttg agacgagatt tgaacgcgcc ctccactgca tggcagaaga ccgagctggc
     2761 gcggcatccg cagcggccgc agacgctcga ctacatcgag cgggtcttca ccgatttcag
     2821 cgaaatccac ggcgaccgcg gtttcgccga tgacgcagcc atcgtgtgcg gcatggcccg
     2881 cttccacggc gacgaagtgc tcgtggtggg cacgcagaag ggccgggaca tgaagcagcg
     2941 cgtgtaccgc aacttcggca cgccgcatcc ggagggatat cgcaaggcga tccgcatcat
     3001 gaaggtggcg gagaagtttc accgaccgat ctttacgctg gtcgatacgg atggcgccta
     3061 tcccggcatt cacgcagaag aacgcgggca ggcggaggc
//
LOCUS       DASPUL010000396         5269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_623026, whole genome shotgun sequence.
ACCESSION   DASPUL010000396 DASPUL010000000
VERSION     DASPUL010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623026"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VEJ00_09635"
     CDS             complement(<1..297)
                     /locus_tag="VEJ00_09635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXX01462.1"
                     /translation="MLEHNYWLTTVNMPTCDAARSLPDVVDVAVIGSGFTGLSAARTL
                     AKRGANVAVLEGETIGWGASSRNGGMVLTGMKLGVNKLISMYGRERTQRMYAASL"
     gene            638..2593
                     /locus_tag="VEJ00_09640"
     CDS             638..2593
                     /locus_tag="VEJ00_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXX01463.1"
                     /translation="MALRCLLFSTDRRTADPVCQVLAGLEIEAEYCAEAPVAVERLTA
                     QPVQIVIIDWENQPEAGFLLSAARDRKATERPLTLAIVSDDASVPKALQAGANSVLRK
                     PIVVNQVKETLTTARALLKAKETPPASPAAASSSNRPAPVASSPIGGEPTLRAGEFLQ
                     STSPAPGTQWDVENSGLMTAPEIDPLKELEPMAAAVENENPVPAPPPIPAPEEPRGLQ
                     WYLNSRAGGAAQAPVHTPAPPAPAKPELLGYDQTPQAAPVPEGGDLAAGQWSPAASPH
                     VSQEQQAEAKLFAYITGGGEESRREESGSRFRLGKGAIIAAAALAACAIMAAPQAPWH
                     GSLRGLWGRARQSTHAWLNPQPVTTPAAPATHEDFARAGDEYKLPVAETIPDATTDPS
                     QIRVVPVVDPTAKKPNNAGANADSTAGQESVTDQNANTVAPGDQTHSDGTQSDGTQGA
                     AGQPLQTTPATTQPAGGDASMSAPVSGTAAQVSVSPQPRPNVTPAVSAPVQPVAVQPT
                     VLKTPPARVITPTSSTTVPPIPSSLKSQMATMVPDASGNKPVEAAMPTIEPVQVPEAT
                     ERALLAEQPAIAYPANVKGQQGMVILDVLIGRDGTIQDAKFLQGSLAFARAAIDGVKQ
                     WKFKPYTMNTRPVAVETHLTISFVPGS"
     gene            2820..4034
                     /locus_tag="VEJ00_09645"
     CDS             2820..4034
                     /locus_tag="VEJ00_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXX01464.1"
                     /translation="MKIKAVVLVSSFAVLLFVVVGNYGGVHASSNDGSFHQLQVYSEV
                     LTRVQSEYVEEPNIPKVTDGALHGLLESLDSNSSYMSAEDYKAYKEHKTEAKGGIGAV
                     ISKRFGYADVVAVLPGSPAEKAGIEPTDIFESIEGRSTRDMSLPEIRNLLAGTPGSNV
                     NVEVVRARRAEPQKIVIARDVTPVPPVTDKQMDDGIGYVKAQALTRGKAQEIAAKIKA
                     LERLGAKKILLDLRNCAEGEENEGIAVANLFLNHGTITYLQGQKYPREAFNADPAKAI
                     TTLPVAVLVNRSTAGAAEIVAAAILENARGDVIGDKTFGDGSVQKTIDLPDGGALILS
                     VAKYYSPIGKAIQDAAVTPNVVVADADQDDGAGVDDEDQAPPQQQQEEQPKKTIDDQL
                     NKAVEVLKSRTS"
     gene            4040..4252
                     /locus_tag="VEJ00_09650"
     CDS             4040..4252
                     /locus_tag="VEJ00_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HXX01465.1"
                     /translation="MADFRNQIDSLSAAEKVDLLDAVWQSLEADAASLTDSQRAELDF
                     RIARHEKDPSNVLPWEQVKANLFKKP"
     gene            4249..4542
                     /locus_tag="VEJ00_09655"
     CDS             4249..4542
                     /locus_tag="VEJ00_09655"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXX01466.1"
                     /translation="MTFPVVWTPEAQADLEEAYAWYADRGSQVAERFALAVEATVQAI
                     AGNPQQFPVVHRGRRRAGVRRFPSGIIFEVQEERIVVMACFHGRRNPQRWRLR"
     gene            4633..>5269
                     /locus_tag="VEJ00_09660"
     CDS             4633..>5269
                     /locus_tag="VEJ00_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HXX01467.1"
                     /translation="MRSIYEELPPVEIGGRRVVGRVLFGLLVLLAALVGATAGLLLVY
                     TTDLPQVDALEAYRPSSITEIYDDHGRVVGSFALQRRVVASYDDFPPVLRDALVSIED
                     KDFYRHSGINFWRIVGAAYRDIESGGKVQGASTLTMQLARNLFLSPDRSFHRKVQEAM
                     LAIQIERHFTKPQIFTLYANQIFLGHGVYGFEAASEFYFSKPAKQLTLDEAA"
BASE COUNT         1044 a         1574 c         1730 g          921 t
ORIGIN      
        1 cagcgatgcg gcgtacatgc gctgcgtgcg ctcgcgcccg tacatggaga tgagcttgtt
       61 cacgccgagc ttcatgccgg tgaggaccat gcctccgttg cgcgagctgg cgccccagcc
      121 gatggtctcc ccttcgagca cagcaacatt ggcgccgcgc ttggccagcg tgcgcgcggc
      181 cgagagtccg gtgaagcctg aaccgatcac cgccacatcc acgacgtcag gcagagaacg
      241 ggccgcatcg cacgtgggca tattgaccgt cgtcagccag tagttgtgtt cgagcattgg
      301 gcgcaatcct tacccggctt tctttgcgtc ttttgcgacc tctgcggttc aggcttgctt
      361 ctggctgtgc gctagaagcc tttaccgcag aggtcgcgaa gaaaaagccg caaagtacgc
      421 aaagcggagc ttttcccggg gcttcgtcgc tcttcaccgt cgatgatgac gacccccgca
      481 gtttgcatga agcgattgtg agcgaccgag cattctagcg cgcggccgcc ccgccagtac
      541 atgccccttc gggtgctgac gatgcgctaa acaggtgact ttgttccacc tcggtaatct
      601 tgtcggcggt taccggcaac cctaagctga gagggcaatg gccctgcgct gcctcttgtt
      661 ctccaccgac cggagaaccg ccgaccctgt gtgccaggtg ctggcgggtc tcgaaatcga
      721 ggccgaatac tgcgccgaag ctcccgtcgc ggtcgaaaga cttaccgccc aacccgtaca
      781 gattgtcatc atcgactggg aaaaccagcc ggaagcggga ttcctgctct cggccgcccg
      841 cgatcgcaag gctacggaac gaccgctgac gctggcgatc gtgagcgacg atgcgagcgt
      901 acctaaggcg ctgcaggcgg gcgcgaactc ggtcttgcgc aagccgatcg tcgtgaatca
      961 ggtgaaagaa acgctgacca cggcgcgcgc gctgttgaag gcgaaggaaa cccctccggc
     1021 gtcgcccgcg gcggcgtcct cgtccaatcg gccagcgcct gtggcttctt ctccgatcgg
     1081 cggcgaaccg acgttgcgcg ccggcgaatt cctgcaatcg acctctcccg ctccgggaac
     1141 gcagtgggac gttgaaaact ccgggttgat gacggctccg gagatagatc cgctgaaaga
     1201 gctggaaccg atggcggcgg cagtggaaaa tgaaaatcct gtcccagccc cgcctccaat
     1261 cccggctcca gaagagccgc ggggcttgca atggtatttg aattcgcggg cgggaggggc
     1321 tgcgcaagct ccagtccaca cgccggcgcc tccagcacca gccaagccgg agttgctcgg
     1381 ttacgaccag acgccgcagg ccgctcctgt tcccgaggga ggcgatttgg ctgccggcca
     1441 gtggtcgccg gcggccagtc cgcatgtctc gcaagagcag caggcagaag cgaagctttt
     1501 tgcttacatc acgggcggag gcgaggaatc ccgccgggaa gaatccggct cacggttccg
     1561 gctgggcaag ggggcgatca tcgcggccgc ggcgctggcc gcctgcgcga tcatggcagc
     1621 gccacaagcg ccgtggcacg gaagtctgcg ggggctgtgg ggacgagcgc ggcaatcgac
     1681 tcacgcctgg ctgaatccgc agccggtcac tacgccggca gcgccggcca cccatgagga
     1741 ttttgcccgc gcgggcgacg aatataagct gccggtcgca gagaccattc ctgacgcgac
     1801 taccgatccc tcacagatcc gcgtcgtgcc cgtagtggat cctacggcga agaagccgaa
     1861 caacgcggga gcgaatgcgg actctacggc gggacaggag agtgtgacag atcagaatgc
     1921 gaacacagtc gcgcccggcg atcagacgca cagtgatgga acgcagagcg acgggacgca
     1981 aggcgcggcc ggacagccgc tgcaaaccac tcccgccaca acgcagccgg caggcggaga
     2041 cgcttcgatg tctgcgccgg tgtcgggcac ggcagcgcag gtcagtgttt ccccgcagcc
     2101 acgcccgaat gtaacgccgg ccgtttcggc gccggtacaa ccggttgccg tccagcccac
     2161 agtcttgaag actccgccag cgcgggtgat cacgccgacg agctcgacca cggtcccgcc
     2221 cattccttcc agcctgaagt cgcagatggc aactatggtg ccggacgcca gcggcaacaa
     2281 gccggtcgag gccgcgatgc ccaccattga gccagtgcag gtaccggaag ccacggagcg
     2341 tgcgttgctg gccgaacaac cggcgattgc ctatccggca aacgtgaagg gtcagcaagg
     2401 gatggtgatt ctggatgtcc tgatcgggcg cgacggcacg atccaggatg ccaagtttct
     2461 gcagggttca ctggccttcg cgcgagcggc gatcgacggc gtgaagcagt ggaagttcaa
     2521 gccctacacc atgaacacac ggccggtcgc ggtcgagacc catctgacca tcagctttgt
     2581 tccgggatcg taagcgctgg agcggcggac gcctcgtccg cgccccgaac gagggcgtcc
     2641 ggggctacat ggccttgaat ccagaccttt tccgccagaa cagtctaaga atctgtgcgg
     2701 cgttacctcc ggggctcaaa tcggagcagc caggggtttg aaatcggcat ctagcagata
     2761 taatgagata taagttcagc ggaagtactt ccttccagaa aacggccaaa cggatgtcca
     2821 tgaaaatcaa ggcagtagtt cttgtcagct cctttgcggt gttactgttc gtcgttgtcg
     2881 ggaactacgg aggcgtgcac gcttcctcga acgacggatc cttccatcaa ctccaggtct
     2941 acagcgaagt gctcacgcgg gtgcagagcg agtacgtcga ggagccgaac atcccgaagg
     3001 tgaccgatgg agcgctgcac gggctgctgg agtcgctgga ttcgaactcc agctacatgt
     3061 cggccgagga ttacaaggct tataaggaac acaagaccga ggccaagggc ggcatcgggg
     3121 cggtgatttc gaagcgcttc ggatatgccg acgtggtcgc ggtgctgccc gggtcgccgg
     3181 cggagaaggc tggaatcgag ccgaccgaca tcttcgaatc gatcgaaggg cggagcacac
     3241 gggacatgtc gctgcctgag attcgcaacc tgctggccgg aacaccggga tcgaacgtga
     3301 acgtggaggt ggtgcgggcg cgccgggccg agccgcagaa gatcgtgatt gcgcgcgacg
     3361 tgacgcctgt gccgccggtg accgacaagc agatggacga cggcatcggg tatgtgaagg
     3421 cccaggcgct gacccggggc aaggcgcagg agatcgcggc gaagatcaag gcgctggaac
     3481 ggttaggggc gaagaaaatc ctgctcgacc tgcgcaattg cgccgaaggc gaagagaacg
     3541 agggcatcgc ggtggcgaac ctgttcctga accacgggac gattacgtat ctgcaggggc
     3601 agaagtatcc gcgggaggcg ttcaacgccg acccggcgaa ggcgatcacg accctgcccg
     3661 tcgcggtgct ggtgaatcgc agcacggcgg gcgcggcgga aatcgtggcg gcggcgattc
     3721 tggaaaacgc acgcggcgat gtgatcgggg acaaaacttt cggcgacggc tcggtgcaga
     3781 agacgattga tctgccggac ggcggagcgc tgattctttc cgtggcgaag tattactcgc
     3841 cgatcgggaa ggcgattcag gacgcggcag tcacgcccaa tgtggtggtc gccgacgccg
     3901 atcaggatga cggggccggt gtcgatgatg aagatcaggc gccgcctcag cagcagcagg
     3961 aagagcagcc caagaagacg atcgatgatc agctgaacaa agccgtggaa gtgctgaaga
     4021 gcagaacgag ctaggatgta tggccgattt ccgaaatcag atcgatagcc tctcggcggc
     4081 agagaaggtg gatttgctgg acgcggtgtg gcaaagcctg gaggcagacg ccgcatcgct
     4141 gactgactcg caacgcgcag aacttgactt ccgcatcgct cggcatgaga aagacccgtc
     4201 caacgtcttg ccctgggagc aagtcaaggc aaacctgttt aagaaaccgt gacctttccg
     4261 gttgtctgga cgcccgaggc gcaggcggac ctcgaagaag cgtacgcctg gtatgccgac
     4321 agaggctcgc aggtagcaga gcgtttcgcg cttgcggtcg aagccacggt tcaggcaatc
     4381 gcagggaatc cacaacagtt tcccgttgtg catcgtggcc ggcggcgagc cggcgtgcgg
     4441 cgttttccct ccggaatcat cttcgaggtg caggaggagc ggattgttgt aatggcctgc
     4501 ttccacggcc ggcgcaatcc gcagcgctgg cggttacgct agtgcatcct gggcgctccg
     4561 ttcgtgcact cggcccccgg acgaatgggt ccgtggccgc atcattcgat gctattctga
     4621 gctcgactcc cgatgagatc gatttacgaa gagctgccgc cggtggagat cggcgggcgg
     4681 cgggttgtgg gccgcgtgct gttcgggctg ctggtgctgc tggcggcgct ggtgggggcg
     4741 acggccgggc tgctgctcgt ctacaccacc gatttgccgc aggtcgatgc gctcgaagct
     4801 taccggccca gttcgattac cgaaatctat gacgaccacg ggcgcgtcgt cggctctttt
     4861 gcgctgcagc ggcgcgtggt cgccagctac gacgattttc ctcccgtgct ccgcgacgct
     4921 ttggtttcca tcgaggacaa ggacttctat cgccactccg gcatcaattt ctggcgcatc
     4981 gtcggggcgg cgtatcgcga catcgagtcg ggcgggaagg ttcagggggc gtcgacgctc
     5041 accatgcaac tggcgcgcaa tctttttctg tctcccgacc gctcgtttca tcgcaaagtg
     5101 caggaggcga tgctggccat ccagatcgag cggcacttca ccaaaccgca gattttcacg
     5161 ctgtatgcca atcagatttt tcttggccac ggcgtgtacg gattcgaggc ggcgtcggag
     5221 ttttatttca gcaagccggc taaacagctg acgctggatg aagcggcaa
//
LOCUS       DASPUL010000397         4010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_751560, whole genome shotgun sequence.
ACCESSION   DASPUL010000397 DASPUL010000000
VERSION     DASPUL010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751560"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..1018)
                     /locus_tag="VEJ00_09665"
     CDS             complement(311..1018)
                     /locus_tag="VEJ00_09665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXX01468.1"
                     /translation="MGGNSAQDTSTGLKNRALGEVVNESSGKKIRIVFGLALALGLPT
                     LMMRKSEHEFIDRSHLIGYEIVWWAVVASVLLYVLFVERRPLSSVGYRRIGWSSVGIA
                     VAAAVLLMAGLALIFYVVLPALHLTMAQQVNKLLTLPLWCRLILVIRGVVAAELFYRG
                     YGIERLQELTRSRAVAGITSCALFTLANAPSGGWASIIFAGFAGIILTVLYIWRRNVW
                     VTMITHFVVDGVGVLTA"
     gene            1522..>4010
                     /locus_tag="VEJ00_09670"
     CDS             1522..>4010
                     /locus_tag="VEJ00_09670"
                     /inference="COORDINATES: protein motif:HMM:NF021665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G8 domain-containing protein"
                     /protein_id="HXX01469.1"
                     /translation="MRRQYRSFLFALLVPTFFLVSALGVVVAAEEEGSQAVKQELWSN
                     PATWPDGKVPGAEDTVTIERGKDVLLDVSPPSLGGLQINGKLSFSDKADIELTTESIV
                     VHGELEIGTEAKPHTRKATITLTNNVPGNDRGIMVAGGTLNLHGDRTNSWTKLAGTAK
                     AGSARIEVLNASGWRKADVIVLASTDFDSTQTEKRTIAAIKGNVITLDQPLKYMHFGK
                     ITFGVDERGEVGSLTRNILIQASEDAERSYFGGHIMAMAGSKMYVSGVELNRMGQNMH
                     LARYPIHWHLLGEGQGQYIENSAIHDTYSRCVTVHGTNDVRVENNVAYNTVGHCYFLE
                     DAVETGNQFIHNLGMMTKCHPDGKPCVPTNLGPIGTEPKTVFDPAGQNAKDVLIPSDN
                     TASTFWITNPDNIFRDNVAAGSEATGFWLALPQHPTGAFEGTEVSKTTWPRRTRLREF
                     KGNTAHSNFDGFMFDRGPQPNGHISPGGHMALANPADGNSPIVETVIEDFTSYKNRNS
                     GMWARGEMDTFKNLKLADNAIGYTHASGNFGRSDFTSRVVDSLFVGETENIGNPSAPA
                     EMAYGRSLPEPTLPDFPIRGYEFYDYRHDLDNDTFVNYQDNATRKTGAISYLLFTSFG
                     MSSNNTVQRAKFINAKPVYFPPMESKWTNDDYGNGSYKTSVFHDADGSVSGVADSYIL
                     IDDQDDEMAIDDACVVKPTWNAAVCKGDVGRFAVGTPGEGGGGGVVFPPRSFNGAGGP
                     GAARTAPAAPVATAGPGAGAGPATAAATGAGPTAGAYPAAGPGPAGPGLAATRRDPGS
                     VFGRPSGPPQPPVILSRNGKEFTLNGATNIRA"
BASE COUNT          939 a         1184 c         1151 g          736 t
ORIGIN      
        1 tacaccatca cgtaccaggg gcgatcgtcg tacacggtga gtatcatgga attccgcaac
       61 agcaaggtcg tccacgaaac acagtatttc tgcgatccgt tcgaggcgcc ggcttggcgg
      121 aagcaatggg tccagcagac cgtgtgacgc cgcactgagg ttgaggacat ttgcttggcg
      181 gatagcaaac cattgacgcc gcgtcccgat tagcggggtt accggaagct cgctctccgc
      241 cagcaccgaa tgtccggtta atccgactaa tcaggacttt ggctagcttg tcgaaaccga
      301 tgccagcgga ttaggcggtc aggacgccga caccgtctac gacgaaatgc gtgatcatag
      361 tgacccacac gttccgacgc cagatataga gcacggtgag gattatgccg gcgaagcctg
      421 cgaaaatgat cgaggcccag ccgccggaag gcgcattcgc caatgtgaaa agggcgcagg
      481 aagtaatacc ggcgacagcg cggctgcgtg tgagttcctg gaggcgctcg atcccataac
      541 cgcggtagaa aagttccgcc gcaacaacgc ctcgaatgac caatatgagt cggcaccaga
      601 gcggaagtgt gagcaacttg tttacttgct gggccatagt gagatgaagg gcggggagta
      661 cgacgtagaa gatgagagcc aatcccgcca tgagcagcac cgcggcagcg accgcgatgc
      721 cgacgctgct ccatcctatc cggcgatagc cgaccgacga tagcggccgc ctctcgacaa
      781 aaagcacgta caggagcaca gaagctacga ccgcccacca tacgatttcg taaccaatca
      841 gatgacttct gtcgataaac tcatgttctg atttcctcat catcagagtc ggaagtccca
      901 gcgcgagagc gagcccgaag acgatgcgaa tcttttttcc agagctttcg ttcacgactt
      961 cccccagcgc cctattcttc aaaccagtgc tcgtgtcctg agcagaattg cctcccatag
     1021 agcctcctct gcagggttag atagcgagcg cggactgagc ttcttaagga ccgagccgag
     1081 aacccattca caaggtcgta cagtggcggg agttttacat cagaaacgtc ggctgtcaat
     1141 gcgaagtcct gagaatccgg gtaatcggac aaatggaatt ttggtacggg aagtgtccaa
     1201 ttatcccact taccaggcgt tgctgttaaa gtgctacgtc gactgcggat ctttacggag
     1261 aaatcagatt caacctacac cttgctccgc gcacgatcat cgatatgtaa agcgaacttc
     1321 gtaactaatc gtgccacaag aaataatcca cttacgattg ttaagacgcc aacagggtac
     1381 atcgacccaa agcggccttt actcagcagg cgacagaaga gctataatgc cgcgtcatga
     1441 gccccggggg gagcgtaatc gggcgaagat ccgcgcgccg cgaagatgcg gggtcagctt
     1501 tgaatacgag ggattgatga catgagaaga caatatcgct cgttcctttt tgcgttgctg
     1561 gttccaacat tcttcttggt cagcgcactc ggcgtcgttg tggcggctga ggaagaagga
     1621 tcgcaggcgg tgaagcagga actctggtcc aatcctgcca cctggccaga cgggaaggtg
     1681 cccggcgcag aggacacggt caccattgaa agaggcaagg atgtgctcct cgacgtcagt
     1741 ccgccgtcgc tgggcggctt gcaaatcaat ggcaaactca gcttctcgga caaagccgac
     1801 atagagctga ccaccgagtc gatcgtcgtg cacggggaac tggaaatcgg caccgaagcc
     1861 aaaccccaca cgcgcaaggc gaccatcacc ctcaccaaca atgtccccgg taacgaccgc
     1921 gggatcatgg tcgcgggcgg aaccctgaac ctgcatggtg accggacgaa ttcctggacc
     1981 aaactcgctg gaacagccaa ggccggtagc gccaggatcg aagtattgaa tgccagtggc
     2041 tggcgaaaag cggacgtgat cgtccttgcg tcgaccgact tcgattccac gcagacggag
     2101 aagcgaacca tcgccgccat taaagggaac gtgatcacgc ttgatcagcc cctaaagtac
     2161 atgcatttcg gcaaaatcac gttcggcgtc gatgagcgtg gtgaagtcgg ctcgctgacg
     2221 cgaaatattc ttatccaggc ttcggaggac gcagaacgct cctacttcgg ggggcatatc
     2281 atggcgatgg ccgggtctaa aatgtatgtc tccggcgtcg aattgaatcg catgggtcag
     2341 aacatgcatc tggcccggta tccgatccac tggcatctgc tcggcgaagg ccaagggcag
     2401 tacatcgaaa attcagcgat tcacgacacc tacagccgtt gcgtgacggt gcatggcacc
     2461 aatgacgtgc gagtggaaaa caacgtggcc tacaacaccg tcggtcactg ctacttcctg
     2521 gaagacgccg tcgaaacggg caaccagttc atccacaacc tgggaatgat gaccaagtgt
     2581 catcctgacg gcaaaccctg tgttccgacg aacctcggcc ccatcggaac ggagcccaaa
     2641 acggtcttcg atccggccgg ccagaacgcc aaagatgtcc tgatcccttc cgacaatacg
     2701 gcgtcgactt tctggatcac gaatccggac aatattttcc gggacaacgt ggccgcgggg
     2761 tccgaggcga cgggcttctg gttggcattg ccgcagcatc cgacgggcgc gtttgaaggc
     2821 acggaagtca gcaagacaac ctggccacgt cgaacccggc tgcgagaatt caagggcaac
     2881 accgcccatt cgaactttga cggtttcatg ttcgaccgcg gccctcagcc caatggccac
     2941 atcagtcccg gcggccatat ggccctagcc aacccggctg acgggaacag ccccatcgtg
     3001 gagacggtga tcgaggattt caccagctac aagaaccgga atagcggaat gtgggcccgc
     3061 ggcgagatgg acaccttcaa gaacctgaag ctggccgaca acgccatcgg ctacacgcat
     3121 gcgtccggca acttcggccg ttccgacttt acgtcacggg tggtcgactc gctgttcgtg
     3181 ggcgaaaccg agaacatcgg caaccccagc gcgccggcgg agatggcata cggccgcagc
     3241 ttgccggagc ccacacttcc ggatttcccg atccgcggct acgagtttta cgattaccgc
     3301 catgacctgg acaatgacac cttcgtgaat taccaggaca acgccacccg caagacggga
     3361 gcgatctctt acctgctgtt caccagcttc ggaatgagct ccaacaacac cgtacagcgc
     3421 gcgaaattca tcaatgccaa gccggtgtat tttccgccga tggagagcaa gtggaccaac
     3481 gatgattacg gcaacgggag ctacaagaca tcggtgttcc acgacgcgga cggctcggtc
     3541 agcggtgtgg cggactccta cattctgatc gatgaccagg atgacgaaat ggccatcgac
     3601 gacgcttgtg tggtgaagcc gacctggaat gccgcggtgt gcaagggcga cgtgggacgt
     3661 tttgctgttg gcacccccgg cgagggcggc ggcgggggcg tggttttccc ccctcgtagt
     3721 tttaacggcg ccggtggccc aggtgctgcc agaaccgcac ccgcggctcc cgtcgcaact
     3781 gccggtccgg gcgctggcgc aggtcctgcc acagccgcag ccaccggtgc tggcccaact
     3841 gccggggctt acccagctgc cggtcctggc cccgccggtc ctggcctagc ggcaactcgt
     3901 cgcgatcccg gctcagtgtt tggccggccc agcggacctc cccagccgcc agtcattctc
     3961 agccgcaatg gcaaggagtt caccctaaac ggtgcaacca acatacgcgc
//
LOCUS       DASPUL010000398         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_906067, whole genome shotgun sequence.
ACCESSION   DASPUL010000398 DASPUL010000000
VERSION     DASPUL010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906067"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VEJ00_09675"
     CDS             <1..323
                     /locus_tag="VEJ00_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01470.1"
                     /translation="QEARTAFINRLVERRYLGHGGWRSVLITLMSNKTASDSANIRMP
                     PAIDRRMKARLYAQMALCFEKQGLMAQAIESWHFAAIAEGLFVPTDEHYQPGRKQGWS
                     DAAS"
     gene            complement(336..1526)
                     /locus_tag="VEJ00_09680"
     CDS             complement(336..1526)
                     /locus_tag="VEJ00_09680"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HXX01471.1"
                     /translation="MNSGNDTGQSPDRNSIHRAAVRLRKWAIEQALPLWANAGFDRKS
                     GRFEERLSLSGQPVFDVQQRLMVQARQIYSYGLAARRGWHARAATLLAESYASMIRDY
                     QRSDGRDGWVFSVHRDGSVADSRRDLYAHAFVLLAVASYIMATGRREALAVADEILDF
                     LDQHMAAPLAGGYIDAFPAVDALRRQNPHMHMLEGLLSLWTSSGEERYLARAKNIFEL
                     FARHFFLPGYGVLAEYFDPGLNPAEGVKGRIVEPGHHYEWIWLLRWLETAGGCTVQAY
                     VDALYRHADAYGYDAQGLVVDELLSDGTIHTRSRRAWTVTEAIKANLVEAARGRPGAA
                     GKAASMVDRLHDYFLTKHPPGGWIDRLDEDGRPATDFMPASTLYHILCALDELDRFDA
                     ECRS"
     gene            complement(1523..>2442)
                     /locus_tag="VEJ00_09685"
     CDS             complement(1523..>2442)
                     /locus_tag="VEJ00_09685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXX01472.1"
                     /translation="ASMLAPRTPKPVPKPGWRFAEEYYDHRRWFACRRGALWEAAAKD
                     DISLPLVLPWYDGMIVDLTLGNDNSLCLYVCGSFEPNEFAFLHTFLKPGMVFIDVGAN
                     DGYYTLFAARRIGPSGLVIAFEPSTRERINLRRNLKRNRLENVRVAACALGAATGDAE
                     LHLAHGVHAGHNTLGKFAHDDVVGASLERVDVVTLDQISVDFGLQKIDFVKIDVEGAE
                     ASVVAGARKVLSKMRPLMLLEINDGALSAQGIGADALLDSLRTEFMYEILIFSATTGL
                     LELLGEGTPLSANIVAVPKERLTELLGQA"
BASE COUNT          477 a          763 c          746 g          456 t
ORIGIN      
        1 agcaggaggc gcgaaccgcg ttcatcaatc gtctggtcga acgccgttac ctgggacacg
       61 gaggctggcg atccgtcctc atcacgctga tgtccaacaa gaccgcgtcg gattcggcta
      121 atatccgcat gcctcccgca atcgatcgcc ggatgaaggc acgtctatat gcccaaatgg
      181 cgctgtgctt cgagaagcag gggctcatgg cgcaggcgat tgaaagttgg cacttcgccg
      241 cgatcgcgga ggggctgttc gtgccaacgg acgaacatta tcaacccgga cggaagcaag
      301 gttggtcgga cgccgcatct tgagccagcc ggcgtctagc tgcggcattc agcatcgaag
      361 cggtccaatt cgtcgagcgc gcaaaggatg tgataaaggg tgctcgcggg catgaaatcc
      421 gtcgccggcc ggccgtcctc gtccagcctg tcgatccatc cgcccggcgg atgtttggtg
      481 agaaagtaat cgtggagccg atcgaccatg gatgcggcct tgccggccgc gcctggcctg
      541 ccgcgcgccg cctccacgag attggccttg atggcttccg tcacggtcca ggcccgtcga
      601 gagcgggtgt ggatggtgcc gtcgctcaac aactcgtcaa ccacgaggcc ttgggcgtcg
      661 tacccgtagg cgtcggcgtg gcgataaagc gcatcaacat aggcctgtac cgtgcatccg
      721 cccgccgttt ccagccaacg caacagccag atccattcgt aatggtggcc cggctcgaca
      781 atgcgtccct tgacgccttc cgccggattc aacccaggat cgaaatattc cgcgagcacg
      841 ccatagcctg gaaggaagaa gtgccgcgca aaaagctcga aaatgttttt cgcgcgggcg
      901 agatatcttt cttcgcccga actcgtccaa agggacaata gcccctcgag catgtgcatg
      961 tgcggattct gccggcgcag cgcgtcgacg gccgggaatg catcgatata tccgccggcc
     1021 aggggcgcgg ccatgtgctg atcgagaaag tccaggattt cgtcggcgac ggccaaggct
     1081 tcgcgccgcc cggtcgccat tatgtaggaa gcgactgcca gtagcacgaa cgcgtgcgcg
     1141 tagagatcgc ggcgggaatc ggcgacactg ccgtcgcggt ggacggagaa tacccagcca
     1201 tcccgcccat cgctgcgctg gtaatctcgg atcatcgatg cataagactc cgcaagcagc
     1261 gttgccgctc gggcgtgcca gccgcgtcgc gccgcaaggc cgtacgagta gatttgccgc
     1321 gcctgaacca taaggcgctg ttggacgtca aaaaccggct gtccgctcag cgacaggcgc
     1381 tcttcgaagc gtccgctctt gcggtcgaaa cccgcgttcg cccacagcgg cagcgcctgt
     1441 tcgatggccc acttccgtag ccggacggcg gctcgatgaa tgctgtttcg atccggcgat
     1501 tgacccgtgt cgttgcccga attcacgcct gtcccagcaa ctcagtaagc cgctccttgg
     1561 gaaccgcgac gatgttggcg gacagcggag ttccctcccc cagtagttcc aatagtccag
     1621 tcgtcgccga aaatatcaaa atctcgtaca tgaattcagt gcgcaggctg tcgagcagcg
     1681 catcggcgcc aatcccctgt gccgataagg caccatcgtt gatttccagc agcattagag
     1741 gccgcatctt cgacaagact ttgcgagcgc ccgctaccac gctcgcctct gcgccttcga
     1801 cgtcgatctt cacgaagtcg atcttctgga gtccgaaatc gactgaaatc tggtcaagcg
     1861 tgaccacgtc cacgcgctcg aggcttgctc ccacgacatc gtcgtgggca aacttgccaa
     1921 gcgtgttgtg gccggcgtgc actccgtgcg cgaggtgaag ttcggcatct cccgtcgcgg
     1981 cgcccagggc gcaggctgca acccggacgt tttccagcct gttgcgcttg aggtttcggc
     2041 gaaggttgat gcgttcgcgc gtactcggct cgaaagcaat caccaggccc gagggaccga
     2101 tccgccgcgc cgcgaacagc gtgtagtacc cgtcgtttgc gccgacatcg atgaacacca
     2161 tgcctggctt cagaaacgta tgcagaaacg cgaactcgtt gggctcgaaa gatccgcaga
     2221 cgtagaggca aaggctattg tcattgccca gcgtcaggtc gacgatcatg ccatcgtacc
     2281 aaggcagaac gagtggcagg gagatatcgt ccttggcggc ggcctcccac agggcgccgc
     2341 gccggcaggc gaaccagcgc cggtggtcgt agtattcctc ggcaaagcgc cagcccggct
     2401 tggggaccgg ttttggcgtg cggggtgcca gcatgcttgc at
//
LOCUS       DASPUL010000399         4636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1718061, whole genome shotgun sequence.
ACCESSION   DASPUL010000399 DASPUL010000000
VERSION     DASPUL010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718061"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VEJ00_09690"
     CDS             <1..419
                     /locus_tag="VEJ00_09690"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXX01473.1"
                     /translation="NKAAAAAADAPAPSMIGLATPGSGGSLPNIVNSGATTPGPILQT
                     LNISQGVSQGLLMKKVQPVYPRNAMSMRVEGTVQLLATISKSGDISAVKVLSGPAQLT
                     GAAADAVKQWKYKPYLLNGEPVEIQTQVTVVFRLPR"
     gene            complement(461..2017)
                     /locus_tag="VEJ00_09695"
     CDS             complement(461..2017)
                     /locus_tag="VEJ00_09695"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase"
                     /protein_id="HXX01474.1"
                     /translation="MAAMALAMRQAAPYGSVTKFRTQLDVSSLAPFVDPLPVPEVARS
                     TGSRPSPTDSAVQLPYYRIPMRQFETKFHRDLKPARVWGYGSCSPGPTFEARSGQGLM
                     VEWVNELPDTHLFAIDHSLNGAEASKPQVRSVVHLHGGKAPPESDGYPENWYAPGKSA
                     IYHYPNRQEAAMLWYHDHALGINRLNVFAGLLGTFFVRDEFEDALNLPGGKYEIPLMF
                     YDRILDLDAQLNYPVSGDPEKPWVPEVFGNAILINGKLFPYLEVEPRKYRFRVLNGAN
                     GRFFHLSLSNGMVAQQIGTDQGLLPSPVRLERITLAPGERADLVVDFSSQAGSRVALS
                     NDDFAVMQFRVAREGPRDTSSLPVSLRPVPKIAEAAAVKTRTLSLVEVDDKLQRPVTM
                     LLNNAHWDAPVTESPTLDSTEIWELVNTTDDAHPIHLHLVRFQILDRRSFDVGAYWNT
                     GSLKYRAPAVVPDPTEAGWKDTVRADPGMVTRIIARFEGFPGRYVWHCHILEHEDNEM
                     MRPYDVVAPK"
     gene            complement(2151..3731)
                     /locus_tag="VEJ00_09700"
     CDS             complement(2151..3731)
                     /locus_tag="VEJ00_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXX01475.1"
                     /translation="MALLIATTIALGPTATPALAASGTATPIRHLVVIFQENVSFDHY
                     FGTYPKALNPGGEPRFVPAVGTPTVNGLGNALLNFNPNLNPANGTGASNPFRLDRSQA
                     VTNDQDHDYTAEQLAFDAGLMDLFPLNTGTAGPPPSGSGVTQTNGLVMGYFDGNTVTA
                     MWNYAQHFALNDNSYGTTFGPSTPGVVNLVSGQTNGVIKTLNGTGDEVSGGPDGSLTL
                     IGDADPLGDVCSNPARAQARLGSKNIGDLLSAAGITWGSFMGGFNLGVINPNGTSGCG
                     RSSANAVDGVATGDYIPHHSFFNYYASTANFGHTRPASMSEIGHGGAANHQYDLEDFF
                     TAAVAGNLPAVSFLKAQAYQDGHAGYSDPLDEQTFIVNTINFLQLLPTWNSTAVVILY
                     DDSDGWYDHQMGPIVNTSTGPADALTGPGACGTAATALPGLNSVANPHALGRCGYGPR
                     LPLLVVSPWAKENFVDHTVTDQSSVIHFIEDNWLRGQRIGPGSYDALSGPLNNMFDFS
                     HARTDGKLFLDPNTGEKE"
     gene            complement(4099..>4636)
                     /locus_tag="VEJ00_09705"
     CDS             complement(4099..>4636)
                     /locus_tag="VEJ00_09705"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HXX01476.1"
                     /translation="SGNRATVVIHLAQLMATLVVKSDPPGANIYIDGRDMGAKTPAQV
                     SVDKGQHVVLVRKMGYIDETASMQFVLGQTFNFSPTLRELANVDNIRTVGKMSKLFGG
                     KGVEAGQAKVTIHTQPKGAQVAINQHVLDKNSPVDVALDPGNYVVDITLTGYAPIHKV
                     ITAEKGGKVVIDEVMQPQ"
BASE COUNT          938 a         1318 c         1429 g          951 t
ORIGIN      
        1 aaaacaaggc agcagccgca gcggcggacg ctcctgctcc cagcatgatc ggactcgcta
       61 ccccgggatc aggcgggtcg ctgcccaaca tcgtgaatag tggagccacc acacccggcc
      121 cgattttgca gactctgaac atttcgcagg gcgtgtcgca ggggctgttg atgaagaaag
      181 tccagcccgt ttatccgcgg aacgcgatga gcatgcgagt cgaaggcaca gtccagttgt
      241 tggcaacgat ctccaagtct ggcgacatca gtgcggtgaa ggtcctgagc ggaccggcgc
      301 agttgaccgg cgcggccgca gacgccgtaa aacagtggaa gtacaagcct tacctgctca
      361 acggcgagcc tgtcgagatc cagacgcagg tcacggtcgt cttcaggctg ccccgctaga
      421 gcggaccgtc acttcatcaa cgcttcactt catggaagag ttacttcggc gcgaccacgt
      481 cgtagggccg catcatttcg ttgtcctcgt gctccaggat gtggcagtgc cacacgtagc
      541 gtccggggaa tccttcgaag cgcgcgataa tccgagtaac catgccggga tcggcacgca
      601 ctgtgtcttt ccagccggcc tctgtgggat cgggcacgac ggctggcgct ctgtatttca
      661 agcttcccgt gttccagtaa gcaccaacat cgaagctcct gcggtcgagg atctggaagc
      721 gcaccagatg cagatggatg ggatgcgcat cgtcggtggt gttgaccaat tcccagattt
      781 cagtcgaatc gagtgtagga ctctccgtga cgggcgcatc ccaatgagcg ttgttaagca
      841 gcatggttac aggccgctgc agcttgtcgt ccacttccac cagactcagc gtccgagtct
      901 tgaccgccgc cgcttctgcg attttgggaa cgggccgcaa gctcacgggc agggaactgg
      961 tgtcgcgagg accctcgcgc gccacgcgaa attgcatcac cgcaaaatca tcgttgctta
     1021 gcgcaacgcg gctccctgcc tggctggaaa aatcgacgac caggtcggcg cgctcgcccg
     1081 gcgcaagggt gatgcgttca agccgcacag gcgaagggag cagtccctgg tctgtgccga
     1141 tttgctgggc caccattccg ttggatagcg acaggtgaaa gaagcggcca ttggctccat
     1201 tgagcacgcg aaaacgatac tttcgcggct ccacttccag ataagggaac agcttgccgt
     1261 tgatcaggat cgcgttaccg aacacttccg gcacccaggg tttttccggg tcgccggaga
     1321 cgggatagtt aagctgcgcg tcgaggtcga ggatgcgatc gtagaacatc aacggaatct
     1381 cgtacttgcc accaggcagg ttgagggcgt cctcgaattc gtcccgcacg aaaaacgtgc
     1441 caagcaatcc cgcgaaaaca ttcaggcgat taatgccgag agcgtggtcg tgataccaca
     1501 acatggccgc ttcctggcga ttgggatagt ggtagatggc ggacttgccg ggcgcatacc
     1561 agttttccgg atagccgtcg ctttccggcg gagccttgcc gccgtgcagg tgaaccacac
     1621 tccggacttg tggtttgctc gcctctgcac cattgaggct gtgatcaatc gcaaacaagt
     1681 gagtgtctgg tagctcgttg acccactcca ccatcaatcc ttggccgctg cgcgcttcga
     1741 acgtgggacc gggcgagcac gaaccgtatc cccacacgcg agctggtttc aggtcgcggt
     1801 gaaacttcgt ctcgaactgt cgcatcggga ttcgatagta gggaagctgc acagcgctgt
     1861 cggtggggct gggccgtgat ccggtggacc gggcgacctc gggaaccggg agcggatcaa
     1921 caaacggagc cagggaactc acgtccagct gagtccgaaa cttggtcacc gatccgtagg
     1981 gggcggcctg ccgcatggcc aaagccatcg ccgccatgcc gccatgctga agaaacaatc
     2041 gccttgtggg ggacagaggt attctcccgg agcaaacaat cggacgggca tgcaaagcac
     2101 tggtctttgc atgcccgcgt cagtcgttca gacgattcta cagttttctg ctattccttt
     2161 tcgccggtat tgggatccag gaacagcttg ccgtcggtac gggcgtggct aaagtcgaac
     2221 atgttgttaa gagggccgga gagagcatcg tacgaacccg gcccgatgcg ctgtcctcgc
     2281 agccagttgt cctcgataaa gtgaatcacc gagctctgat cagtcaccgt gtggtccacg
     2341 aagttctcct tggcccacgg cgagacaacc aataacggca gccttggccc gtagccgcag
     2401 cggcccaggg cgtgcgggtt ggctacggag ttgagaccgg gcaaggccgt ggctgctgtt
     2461 ccgcaggcgc cgggaccggt gagggcgtca gcgggacctg tcgaagtgtt gacgataggg
     2521 cccatctggt ggtcgtacca cccgtcggag tcgtcgtaga ggatgacgac cgcggtgcta
     2581 ttccacgtcg gcagaagctg caggaagttg atggtattga cgatgaacgt ctgttcatcc
     2641 aggggatccg agtagccggc atggccgtcc tggtaggcct gtgctttcag aaagctgacc
     2701 gccggcagat tacccgcgac agcagcggtg aagaagtcct caaggtcgta ctggtgattg
     2761 gctgcgccgc catgtccgat ctcagacata gaagcgggcc gagtgtggcc aaagttggcg
     2821 gtcgaggcgt aatagttgaa gaacgagtga tgcgggatat agtcgccggt ggcaacgcca
     2881 tcgaccgcat tcgcgctgct gcgtccgcag ccgctcgtgc cgttggggtt gatgaccccg
     2941 aggttgaagc cccccatgaa cgatccccag gttatgccag cggcgctcag caggtcgcca
     3001 atgttcttgc ttcccaaccg ggcctgagct cgcgccgggt tggagcacac atcgcccagc
     3061 ggatcggcat cgccaatcag ggtgagagag ccgtcgggac caccgctcac ttcgtcgccg
     3121 gtgccgttga gggtcttaat cacgccgttg gtctggccgg aaaccaggtt gaccacgcca
     3181 ggggtggagg ggccaaacgt ggtcccgtag gagttgtcgt tcagggcgaa gtgctgggcg
     3241 tagttccaca ttgcggttac tgtgtttccg tcgaagtaac ccatgactag cccgttggtc
     3301 tgggtcacgc ccgaccccga gggcggcggt ccggccgtcc ccgtgttaag cggaaacaga
     3361 tccatcagtc cggcatcaaa ggccagttgc tcggctgtgt agtcgtgatc ctgatcgttg
     3421 gtgacggcct gcgagcgatc cagccggaac ggattagaag ctcccgttcc attggcggga
     3481 ttgaggttgg gattgaaatt caacagcgcg ttgccgagac cgttcacagt cggcgtgccg
     3541 acggcaggca cgaacctggg ctcgcctcct gggttcagtg ccttgggata cgttccgaaa
     3601 tagtggtcaa aggaaacgtt ctcctggaag attacgacca ggtgccggat cggcgtcgcc
     3661 gttccggaag cagccagagc tggcgtagcg gtcggtccca gggcaatggt cgtcgcaatc
     3721 aaaagcgcca tcacttttct gggggcttct agtcttagct tctccatctt tacgtcctcc
     3781 tgtgccccgc ggaccagtga ttaaaggcag tggtccacac tcgacgcagc ttttcacgat
     3841 ggtttccgag ccggggttct agagaaccat agcgtcacaa tgtttcgcag gagttgcacg
     3901 agtatgaaag gaaagtgaaa taaactttac attagtgcga cattcccgct gattatccac
     3961 agttctgcct gacataacga cagtgtgatg tgagagcgag ctctaggtct cgaaggatgg
     4021 caggaagcat ccgcccagcg cgcggagaac aattgcaaac gagcaacaga ctcagtgttg
     4081 tgattcggtg aagtcgctct actgcggttg catcacttcg tcgatgacga ccttgccgcc
     4141 tttttcggca gtgatgacct tgtgaatggg cgcataaccg gtgagggtga tgtccacgac
     4201 gtagttgcca gggtcgagcg ccacgtcgac cggcgaattc ttgtcaagca catgctggtt
     4261 gatcgccact tgcgcgcctt ttggttgcgt atgaatggtg accttcgcct gtcccgcctc
     4321 cacacccttt ccgccgaaca gttttgacat cttgccgaca gttcggatgt tgtcgacatt
     4381 ggcgagctcg cgcaaggtcg gggagaaatt aaaggtctga cccagcacga actgcatgct
     4441 cgcagtctcg tcgatatacc ccatcttgcg gaccagcacc acgtgctggc ctttatcgac
     4501 gcttacctga gccggagttt tcgcacccat gtcgcggccg tctatataga tgttggcgcc
     4561 gggaggatca ctcttgacga cgagagtggc catcagctgg gccagatgaa tgacgaccgt
     4621 cgctcggttg ccggaa
//
LOCUS       DASPUL010000400         4878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1440116, whole genome shotgun sequence.
ACCESSION   DASPUL010000400 DASPUL010000000
VERSION     DASPUL010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440116"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VEJ00_09710"
     CDS             complement(<1..372)
                     /locus_tag="VEJ00_09710"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HXX01477.1"
                     /translation="MFAILAGVLYWSDHRKSGADAAKPSADSAPSILKLDENSITKLE
                     LKRKNAPPIQLTKTGSDWEITEPKPFAADQSTVSSLLSTVASLESERLVDEKSSDLRR
                     YGLQQPSLELDVTEKDGKMQCL"
     gene            complement(442..1818)
                     /locus_tag="VEJ00_09715"
     CDS             complement(442..1818)
                     /locus_tag="VEJ00_09715"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HXX01478.1"
                     /translation="MASRWVKARQTKYAAYAATYILIVIAAAVVANTLADRYNKSYDS
                     TANKRYSLSEQTTKIVKGLKQDAVITYFNQSTRFRDGKDLLDQYANLSPKVQVKYVDP
                     DKDPELAREAGIRNFGTTVVQIGTKHEEAKSMTEEGITGAFIRDLKSATRTVCFASGS
                     GEHQLDDSSREGLSRFKELLAKDSYESKSVDLLSKAEVASDCTTLVVAGPTKNYEQPE
                     VNAIKKYVEDGGRAFFMLDPPLKLGRDEIADNDALANLLQSWGVILEKDLILDLNPLG
                     QIAGLGPQVALVTSYASQPIVDQLRGTATGFPLARSLEIKNSDKTSVQKLFDSSATSL
                     ATSNLSSPAVNVDDPKNKKGPLTLAAAGTYNTGKQNSQGRFVVVGSSTWAVNRFIDFN
                     GNDDLASNAVNWLASDEDLISIRPKPPEDRRVTMTGAQLSWVRAVSQFALPFVVMTLG
                     VFVWWKRR"
     gene            complement(1833..2600)
                     /locus_tag="VEJ00_09720"
     CDS             complement(1833..2600)
                     /locus_tag="VEJ00_09720"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX01479.1"
                     /translation="MRNVWIICRKELGSYFVSPVAYILLIMFGLIFGYFFWNALGAFV
                     FESIQSEMSGRSFPMNLNEWIIRPLLSNLSVINLFFIPLVTMRLFAEEKRTGTIELLA
                     TSPVRDIEVILGKWLASVILYACLLLFTAINFAFLFKYGHPDWKPLLVGYFGVFLLAG
                     ALLAIGTFISSLTKNQIIAGAVTFGVCLLLYVFGWVSGYETAAWARVLSYMSVATHIE
                     SFEKGVIDSKDAIFYVTAIFLGLFLTARSMESLRWRS"
     gene            complement(2600..3586)
                     /locus_tag="VEJ00_09725"
     CDS             complement(2600..3586)
                     /locus_tag="VEJ00_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXX01480.1"
                     /translation="MIKVTELTKRYARTTAVDQISFEVAKGQIVGFLGPNGAGKTTTM
                     RMLTCFLPPSSGTATVAGFDVLEQALEVKRRIGYLPETPPIYPEMATLEYLEFVGKLK
                     GLSGTDLAKRVDYVCGRCAIADVKTKLLGKLSKGYRQRVGLAQAIIHNPDVLILDEPT
                     AGLDPKQINETRDLIKDLAGDHTIILSTHILPEVEQTCEQVIIIDKGKLVATDSVRNL
                     QARARGAESVLVEVASHKDGFDPSIVQHKLEQVAGVSRVLCRRQTDAHAVFEVESKKG
                     SFVRGDVARAIVEAGWDLNELRPAAMSLEEVFLKLTGSEAAVLTETPVEVQA"
     gene            3896..>4878
                     /locus_tag="VEJ00_09730"
     CDS             3896..>4878
                     /locus_tag="VEJ00_09730"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXX01481.1"
                     /translation="MRHTLRSSWVLLLFANLVSVSAASLLCAQGTVDERILFNAKVFT
                     GDPQNPYAEAVAIRSDRIVAVGNLPEVAKRASANAQRVDLAGKSLFPGFIDSHSHSID
                     GGLSLIGADASDKVNTLDELPPFVEEAKKAGRGMRGDILEIQGLPLEFWSHPDVLNSR
                     FSTGSYEKQSILLRGMDGHTAWANRVLLQRAGITADFLKTLSTGERSYYGVGKDMQPN
                     GFLVDAGTEKVEKLLPAYTPDELLRAGRAALQYNYSLGITAWLDPLAADYVLQAYKML
                     ADRGELVSEVDAFPRVLARDPAAELAAVQKTREQYKSVPHLHVSGIKIFADG"
BASE COUNT         1008 a         1411 c         1370 g         1089 t
ORIGIN      
        1 caagcactgc atcttgccgt ccttctcggt gacatcaagt tccagcgaag gctgctgaag
       61 accgtagcgc cgtaagtcgg aactcttttc atcgacgagg cgctcggact cgagagaggc
      121 gaccgtcgaa agcaagcttg aaacggtgct ctgatccgcc gcgaacggct tcggctcggt
      181 gatctcccag tccgaacctg ttttggtcag ctgaatcggc ggggcgtttt tccttttcaa
      241 ttcaagtttc gtgatggaat tttcgtcaag tttcaggatt gacggagcag agtcggcgct
      301 cggcttggcg gcatcggcac cggatttacg atgatcggac caataaagaa ccccggcaag
      361 gatggcgaac acagcgattg cagccagcag accacggatc ttcatgcttt acccggccat
      421 ttcaatcgag cgtcgtacag gctatcttcg cttccaccat acgaaaaccc ccagggtcat
      481 caccacgaac ggcaacgcga actggctgac cgcgcgcacc cagctcaact gggccccagt
      541 catggtgacg cgccgatcct ccggtggctt tgggcgaata gaaatcagat cttcgtcgga
      601 cgccagccag ttcaccgcat tcgaagccag atcgtcattg ccgttgaaat cgatgaaccg
      661 gtttaccgcc caggtggagc tgcccactac cacgaatcgg ccctgcgaat tctgtttgcc
      721 tgtgttgtac gttcccgcag cagccaaagt cagcggaccc ttcttgtttt ttggatcgtc
      781 aacgttgacc gctggcgagc tcagattgct ggttgcgaga ctcgtagcgg aactgtcaaa
      841 cagcttctgc acgctggttt tatcgctgtt cttgatctcg agcgagcggg cgagcggaaa
      901 gcctgtcgcg gttccgcgca actggtcaac gatcggctgt gaagcgtagc tggtgaccag
      961 tgccacctgt ggaccaagcc cggcaatctg tccgagcggg ttcaggtcga ggatcaggtc
     1021 cttttcgagg atcactcccc agctttgcaa caggttcgcg agcgcgtcgt tgtctgcgat
     1081 ttcgtcgcgc cccagcttga gcggtggatc gagcatgaag aaagcacggc cgccatcttc
     1141 tacgtatttc ttgatggcgt tgacctctgg ctgttcgtag ttttttgtcg gaccggctac
     1201 cacgagggtc gtacagtcgc tcgcgacctc ggctttcgaa agcagatcga cggacttgct
     1261 ctcatagctg tccttggcga gaagttcctt gaagcgggag agaccttcgc ggctgctgtc
     1321 gtcgagctgg tgctcgccgc ttccgctggc aaaacaaact gttcgggtag cgcttttcag
     1381 atcgcgaatg aaggcgccag taataccttc ctccgtcatg ctcttggctt cttcatgctt
     1441 ggtgccgatc tgcaccaccg tggtcccaaa gttgcgaatg ccggcttcgc gagccagttc
     1501 cgggtctttg tcgggatcga cgtatttcac ctgcaccttg ggagacagat tcgcgtactg
     1561 atccagcagg tctttgccgt cgcggaaacg cgtgctctga ttgaagtagg tgatcacggc
     1621 gtcctgcttc aaccctttga cgatcttcgt cgtttgctcg gagaggctgt agcgcttgtt
     1681 cgcggtcgaa tcgtaggact tgttgtagcg atcggcgagt gtattcgcga cgaccgcggc
     1741 tgcgatcaca atcaaaatgt aagtagccgc ataggccgca tacttggtct gcctcgcctt
     1801 tacccatcgg cttgccatct tgatctcctg tatcaggacc tccagcgcag cgattccatg
     1861 gaccgcgcag tcaagaacaa cccgagaaag atcgccgtaa cgtagaagat cgcgtccttc
     1921 gaatcaatta cgcctttctc aaaggattca atatgcgtcg ccacggacat gtacgacaga
     1981 acccgtgccc aggccgcggt ttcgtatccg cttacccatc caaacacata caaaagcagg
     2041 cacactccga atgtaaccgc cccggcaata atctgatttt tggtgaggct ggaaataaag
     2101 gtcccgatag cgagcagagc gcccgccagc aagaagactc cgaaatagcc gaccagaagc
     2161 ggcttccagt cgggatgacc gtatttgaac aggaaggcga agttgattgc cgtgaacagg
     2221 aggaggcagg catagagaat gaccgaggcg agccacttgc cgagaatgac ctcgatgtcg
     2281 cgcaccggag aagttgcgag cagctcaatc gttcccgtcc gtttttcctc ggcaaacaag
     2341 cgcatggtga ccagcgggat gaagaacaag ttgatcacgc taagattgga aagcagcggc
     2401 cggatgatcc attcgttaag attcatcggg aacgagcgtc cgctcatctc ggactgaatg
     2461 ctttcgaaca cgaacgctcc gagcgcgttc cagaaaaaat atccgaagat cagtccgaac
     2521 atgatcagca ggatgtaggc caccggcgat acgaagtagc tgcccagttc cttccggcag
     2581 ataatccaga cgtttctcat caggcttgta cctcgactgg agtttccgtc agcacggccg
     2641 cttcgctccc cgtgagcttc aggaagactt cctccaggct cattgcagct ggccgcagtt
     2701 cgttcaggtc ccagcccgcc tcgacgatcg cccgcgccac atcgccgcga acgaaacttc
     2761 ctttcttgct ttcgacttcg aatacagcgt gcgcatccgt ctgtcgcctg cagagaactc
     2821 ggctgacccc ggcaacctgc tccagcttgt gctgcacgat cgagggatcg aaaccgtcct
     2881 tgtggctggc cacttccacg agcacggatt ccgccccgcg cgcgcgcgcc tgcagattcc
     2941 gcaccgaatc cgtcgcaacc agcttcccct tatcgatgat gatcacctgt tcacacgtct
     3001 gttcgacttc cgggagaatg tgcgtgctga gaatgatcgt gtggtcgccg gcgagatctt
     3061 tgatcagatc ccgggtctcg ttgatctgtt tggggtcgag cccggcggtc ggctcgtcga
     3121 gaatgagcac atctggattg tgaatgatgg cctgtgctaa tcccacacgc tggcggtagc
     3181 ctttggacag cttgcccagg agcttggtct taacgtcggc gatggcgcag cgcccacaaa
     3241 cgtagtcgac ccgtttcgcg aggtctgttc ctgacagccc cttcaacttg ccgacaaact
     3301 cgaggtattc caaggtggcc atctccggat agatcggcgg cgtctccggt agatacccaa
     3361 tccgcctctt cacttctagg gcctgttcga gcacgtcaaa accagccacc gtggcagttc
     3421 ccgaagacgg gggcagaaaa caggtcagca tgcgcatggt ggtcgtcttg cccgctccat
     3481 tcggaccaag gaagccgacg atctgtcctt tggctacttc aaacgagatt tggtcgacgg
     3541 cagtcgtccg cgcataccgt ttggtaagct ctgtgacttt gatcataagg atttcgcgct
     3601 ggcaagagcg ggcaacacaa ccgttgaccc acaccggaac cgtgcgggaa tagcttatat
     3661 gttagaaagc gtgcccttgc aaagtcaatt ctcgcagcca tctgttcgtt gttctgttta
     3721 atatcgtatc gcgatgtatt tcgacacgcg gacacctgcg aacagcaggg ggtaaggaga
     3781 agcgatgctc tcttcttgtc cgccctcagg aaagccttgt ccggaatctc tacctctgta
     3841 tagttgtttc cttatgggcc agtatggtgg gaattttttc ataagaggtg agcgcgtgcg
     3901 acacacattg cggagctcat gggttcttct gttgtttgcg aacctggttt ctgtttcggc
     3961 ggcttccctc ctctgcgcgc agggaaccgt cgacgagcgc attttattca acgccaaggt
     4021 tttcacgggc gatccgcaga atccgtacgc cgaggcggtg gcgatccgta gcgaccggat
     4081 cgtcgcagtg ggcaatctgc ctgaggtggc aaagcgtgct tcggccaacg cccagcgcgt
     4141 tgacctcgcg ggaaagagcc ttttccccgg cttcatcgat tcccacagcc attcgatcga
     4201 tgggggactg agcctgatcg gcgccgatgc cagcgacaaa gtgaacaccc tcgatgagct
     4261 tccgccgttc gtggaagagg caaagaaagc tggccgcggc atgcgcggcg acattctcga
     4321 gatccagggc ctgccgctcg agttctggtc gcaccctgac gtgctcaact ccaggttcag
     4381 cacagggtca tacgaaaagc agagcattct tcttcgcggc atggacggcc acacggcctg
     4441 ggccaaccgg gttctgctgc aacgcgccgg cattaccgcg gattttctga aaactctttc
     4501 caccggcgaa cgtagctact acggcgtggg caaagacatg cagcctaacg gtttcttggt
     4561 cgacgccggg acagaaaagg tcgagaagct cctgccggcc tacacgcctg atgagctgct
     4621 gcgagcaggt cgagccgcgc tgcaatacaa ctatagcctg ggtatcactg catggcttga
     4681 tcccttggcg gccgattatg tcttgcaggc ttacaagatg ctggccgacc ggggcgagtt
     4741 agtctccgaa gtggatgcgt ttccccgggt tttggccagg gatcccgcgg ccgaactcgc
     4801 cgcggtgcag aaaacccgcg agcagtacaa gagcgtccct catctgcatg tgagcggcat
     4861 caagattttc gcggatgg
//
LOCUS       DASPUL010000401         8399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1848530, whole genome shotgun sequence.
ACCESSION   DASPUL010000401 DASPUL010000000
VERSION     DASPUL010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848530"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VEJ00_09735"
     CDS             <1..640
                     /locus_tag="VEJ00_09735"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HXX01482.1"
                     /translation="YLESHGAQMLVIACNTATALALDRIIAAAHVPVVGVVEPGAEAA
                     AAATKNRRIVVIGTEATVGSHAYRKALEARGLQAREKACPLLVPLVEEGWVEHSITKQ
                     VARIYLAEAFADGFHDADVLVLGCTHYPLLKPLLERVAPGHVNIVDSAESTAHAVASH
                     LRQLLPDVQGEERRAIPRLKFFATDSVEKFRRLGARFLGHPIEDVQHVDLKE"
     gene            736..1410
                     /locus_tag="VEJ00_09740"
     CDS             736..1410
                     /locus_tag="VEJ00_09740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX01483.1"
                     /translation="MHPLDNVIWQALTTRQAQFAEGTGPVRRFQREVTSLTAFEKPDD
                     AVYSALADLVGPRGTAALFLPAPYEARDGWSVVGGAPLLEMVCENGHSSESALEIVEL
                     GPQDSAEMVALAALTKPGPFGARTHELGTYLGIRQGSKLISMAGERLQVPGYTEVSAV
                     CTHPDHLGQGYAGILMTELMKRIRSRGETPFLHVREDNVRAVGLYEKLGFRNRVTVHY
                     VVLRKE"
     gene            complement(1436..2431)
                     /locus_tag="VEJ00_09745"
     CDS             complement(1436..2431)
                     /locus_tag="VEJ00_09745"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXX01484.1"
                     /translation="MGTVEINPNTVAQEPELNLLITDVGFEEPLWKSLFEGLDDFFFP
                     KKLPPLKLESKPEPVKDIWGFYSNWKNGALGSTGVHVLILALIVGGVLLTRRIVRAAP
                     TPHVTVTLIDPGDLPALKPSKTQVGGGGGGGDRDVLQASKGKLPKFSMQQITPPVVVV
                     RNEHPKLAVDPTIVIPPQVQLASNNMPNLGDPMAHAMLPSNGTGSGGGIGSGSGGGVG
                     SGKGPGFGPGEGGGTGGGVFHVGGGVAPPRVIYQPEPEFSEEARKAKYQGVCTLGLIV
                     GVDGRPTNIRVLNSLGMGLDEKAIEAVKNWRFEPATKDGHPVRVEIAVEVDFHLY"
     gene            complement(2729..3205)
                     /locus_tag="VEJ00_09750"
     CDS             complement(2729..3205)
                     /locus_tag="VEJ00_09750"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HXX01485.1"
                     /translation="MVPDSPPPFATPPDLAGVLSDLSRREPIFHRPEFGITRADFEKM
                     TADDFWEVGASGRRYSRAVVLEELERRFSARENDAWETLEFHCRELASGVYLLTYTLV
                     QDPGHDDARRSRRSTIWQRTAEGWKIVYHQGTLVQEEIGSSKDRNKQPPGRTKGEG"
     gene            3365..3685
                     /locus_tag="VEJ00_09755"
     CDS             3365..3685
                     /locus_tag="VEJ00_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXX01486.1"
                     /translation="MSKPSNAAQQLIGSFAPKLVELTDRVLFDDVWERKELSPRDRSL
                     ITVAALIALNRPDQLRFHLGKAVENGVQEQELIESITHLAFYAGWPAAMSAVMVAKEV
                     FSKR"
     gene            3768..4133
                     /locus_tag="VEJ00_09760"
     CDS             3768..4133
                     /locus_tag="VEJ00_09760"
                     /inference="COORDINATES: protein motif:HMM:NF021525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HXX01487.1"
                     /translation="MPAKDSLKDRLSRYRQITITVTGRKSGRAISIPVWFVMDDKLYL
                     LPVEGSNTQWYKNVQKNPVFRIEARGAEEEFRAVPITDAAQVSSVVEKFRKKYGAGDV
                     KKYYSKFDVAVLADSMKAE"
     gene            4188..5183
                     /locus_tag="VEJ00_09765"
     CDS             4188..5183
                     /locus_tag="VEJ00_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXX01488.1"
                     /translation="MKKRTLGNSNLEVSEIGLGCMGMSYGFGPAGDKQEMIALIRSAF
                     ERGVTFFDTAEAYGPFKNEELVGEALAPFRDQVVIATKFGFKIDSKAERAVNGVDSRP
                     EHIRQVAEASLKRLRTDVIDLFYQHRVDSDVPIEDVAGTVKELIQQGKVKHFGLSEAG
                     VRTIRRAHAVQPVTALQSEYSLWWREPEAEVLPALEELGIGFVPFSPLGKGFLTGRIS
                     QDTKFDKNDFRNIVPRFTPENRKANQALVDVLGRFAAQKKVTPAQLALAWLLAQKPWI
                     VPIPGTTKLHRLEENIAAANIELSQGDLRQIDLAASKISVHGARYPEELQKLVGR"
     gene            5453..>8399
                     /gene="ileS"
                     /locus_tag="VEJ00_09770"
     CDS             5453..>8399
                     /gene="ileS"
                     /locus_tag="VEJ00_09770"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HXX01489.1"
                     /translation="MPFELKNTINLPKTAFPMKANLPQNEPKMLERWKQMGIYERIRQ
                     ARRGAQRYILHDGPPYTSGPIHLGTTMNKCLKDFVVKSKTMSGFDAPYVPGWDCHGLP
                     IEIKVDKELGGKKLQMRPADVRAVCRKYAQKYLDLQRMQFQRIGVFGRFDRPYATMNP
                     QYESVVLSTFFSFYENDFVYKGLRAVYWCMHDETALAEAEVEYENHTSPTVFVKYALL
                     DDPARIDPALAGKKVSTIIWTTTPWTLPASMAVAFHPDEEYVALQSGAPGKDDKLWGI
                     DNTEVFIVASKLAADVASKCGLTYPHELAHFPGRKLERLNFQHPFLDRKILGVLADYV
                     TMDTGTGVVHTAPSHGAEDFATGVKYGLDATSNVDEKGILRNGLPEYTGKRVWDANQP
                     IIDLLKSRGALLHTEKTEHSYPHCWRCHNPVIFRATEQWFISMETPMPGGAGKNDTFR
                     TRTLEEITKVKWDPAWGEERLSNMIQTRPDWCISRQRVWGVPIAVFLCEACGKPLNDK
                     AINRKVVELFAKSGADVWFTSEPDSLVPVGMKCPHCGGAKFEKETDIFDVWLESGASY
                     LALIDDEPEYPWPSDLYLEGGDQYRGWFQSSLLCAMGTHQTPPYRNVVTPGWTLDEKG
                     QALSKSLGNAVDPVDIAGRLGGEVVRLWVASVDFREDVVASETLMQRVAENYRSIRNN
                     LFKNALGNLYDFDPQANALSYEKLATLDQYMLRETWALTREVRKWYDEFAFHKIYHRV
                     NHFCVVDLSAFYFDVIKDRLYTYAPNSAGRRSAQTAVWRICEAMVRLLAPILTFTCEE
                     VWQHLPKMPDRAESVHLAAFPEIGDVLGPASVPAEDTQQTKDWETLLSVREQARHRLA
                     NPPHRRLRRAPPSRIRGVRVKPVLDDVEIKSAEIHHAEVIHAVIDLVKGELVIPLANI
                     ARHSPGLAQHVLVERFQLLVRDRVRLRIEVVEIAERVLEQVIADRAVVLRDALHQCFR
                     GDNIFAEVHR"
BASE COUNT         1763 a         2554 c         2439 g         1643 t
ORIGIN      
        1 ctatctggaa tcgcacggcg cgcagatgct ggtcatcgct tgcaacaccg cgaccgcact
       61 ggccctggac cgcatcatcg cagccgctca cgttccggtg gtcggggtgg tcgagcccgg
      121 agccgaggcg gccgcagccg ctacgaaaaa tcgcaggatc gtggtcatcg gcactgaagc
      181 cacggtcggc agccacgcct accgcaaggc gctcgaggcg cgcggccttc aggcccgcga
      241 aaaggcctgt cccttgctgg ttccactcgt tgaagaggga tgggtcgagc actccatcac
      301 caaacaggtc gcccgcatct acctggccga ggcctttgcc gatggcttcc atgatgcgga
      361 tgtgctggtt ctcggctgca cgcactatcc cctgctcaag cccctgctcg agcgcgtggc
      421 gcccggccat gtgaacatcg tggattcagc cgagtccaca gcgcacgccg tcgcttccca
      481 cttgcggcag ctcctgccgg atgtccaggg cgaagaacgc cgggctatcc cccgcctcaa
      541 attcttcgcc accgattcag tcgagaaatt ccggcgtctg ggagctcgtt ttctaggtca
      601 tccaatcgaa gatgtacagc acgtcgatct gaaagaatag ttccaccaca agattcggat
      661 agccctcccc ctgtggcgaa tttactgttt cttccagttc cgccctccat ccaatctggg
      721 gactgcaggg ggaccatgca tccgctcgac aatgtgatct ggcaggcact caccacgcgg
      781 caagcacaat tcgccgaagg gactgggccg gtccgtcgct ttcagcgaga agtcacatcg
      841 ttaacggcgt ttgagaaacc tgacgatgcg gtttatagcg cgctcgccga cctggtcggt
      901 cctcgcggta cggcagccct gttcctgccc gcaccgtatg aagcgcgcga cggatggagt
      961 gtcgtaggcg gggcgcctct gctggaaatg gtgtgtgaga acggtcactc ctcggagtcc
     1021 gcccttgaaa tcgtagaact aggcccgcag gactcggctg agatggtcgc cctcgcagca
     1081 ctgaccaagc ccggcccctt tggagcccgt acgcacgaac ttggaactta tttgggaatc
     1141 cgtcagggca gcaagctgat ctcgatggcg ggagagcgcc tccaggtccc cggatatacc
     1201 gaagtcagtg cggtctgcac acacccggat catctcggcc agggatatgc aggtatcctc
     1261 atgaccgaac tgatgaagcg gatacggagc cgcggtgaaa cacctttcct gcacgtacgc
     1321 gaggacaacg ttcgggccgt cgggctatat gaaaagctcg ggtttcgtaa ccgggtaacc
     1381 gtgcattacg tggtcttgcg gaaggaataa aatccgagag cagacgggca gcttctcagt
     1441 acaggtggaa gtcgacttcg acggcgattt cgacccgcac ggggtgaccg tctttcgtgg
     1501 ccggctcgaa acgccagttc ttcacggctt cgatcgcttt ctcatcgaga cccatgccca
     1561 ggctgttcag cacgcggatg ttggtaggac ggccatcgac gccgacaatc aggcccaacg
     1621 tacagacgcc ctgatatttc gccttgcgcg cttcctcgga gaactcaggc tcgggttgat
     1681 agatgacccg tggcggagcc acgccgccgc cgacgtggaa tactcctcca ccggtaccac
     1741 cgccttcacc cggaccaaac cccggtccct tgccggatcc cactcccccg ccactgccgc
     1801 tgccgatccc gccgcccgag ccggttccgt tcgagggcag catggcgtgc gccatgggat
     1861 caccgaggtt aggcatgttg ttggacgcca gttgcacctg aggtggtatg acaatcgtcg
     1921 gatcgaccgc cagtttcgga tgctcgttgc gcacgaccac tactggaggc gtgatctgtt
     1981 gcatggaaaa cttcggcaac ttgcccttgc tggcctgcag cacatcacga tcgccgccgc
     2041 cgccgccgcc gcccacctgc gtcttggagg gcttgagtgc cggcagatcg cccgggtcaa
     2101 tcagcgtgac cgtgacgtgc ggagttggcg ccgctctcac aatgcgccgg gtcagcagaa
     2161 ctccgccgac gatgagagcc agaattaaga cgtgcacgcc cgtcgagccg agcgctccgt
     2221 tcttccagtt gctgtagaaa ccccagatgt ccttgaccgg ttccggcttc gattccagct
     2281 tgagcggagg aagcttcttg ggaaagaaaa agtcgtcgag tccctcgaac agggacttcc
     2341 acaagggctc ttcaaagccc acatcggtaa tcaggaggtt cagttctggc tcttgagcaa
     2401 ccgtattcgg attaatctcg accgtaccca tgattttcga tctgtacccg tccctgaggg
     2461 agactacaag tctaactcct ctgttatccc ctctttacac agatgaggcg cactggctcc
     2521 aagttgcttg cttttgtctc gattttgtaa atgcggtacc ccttcggtac ctgcagttgg
     2581 aactgcatcc actccctgcc gcttcttcat aaaattgtaa ctctactccg gccttttcga
     2641 cagtgcattt ccttgcccct ggcaactctc ggaaagacat gtccttccta tccacccggg
     2701 gttaccgacg ggtggaccag ctcgtctctc agccttcccc tttggttctc cccggcggct
     2761 gtttgttccg gtccttgctc gaaccgattt cctcctgaac cagggtcccc tgatggtaga
     2821 cgatcttcca gccttcggca gttcgctgcc agattgtgct ccggcggctg cggcgagcat
     2881 catcatggcc cgggtcctgg acgagcgtat aggtcaggag atagacgccg gaagccaatt
     2941 ccctgcagtg gaactcaagc gtttcccacg cgtcgttttc ccgagcggag aatcgcctct
     3001 ccagttcctc cagcaccacg gcgcgcgaat agcggcggcc cgaggctccc acttcccaga
     3061 agtcatccgc cgtcattttc tcgaagtcag cccgggttat gccgaattca gggcgatgga
     3121 aaatcggctc ccggcgggaa aggtcggaga ggactccagc caggtcgggc ggcgtggcga
     3181 aaggcggagg cgaatctgga accatgcggc taggatagca aggcgtctcg gctggcgcat
     3241 ggcacgattc cgcaccaacc ggtgctgagc acatcgggag tgttttgcag atcgaaaaga
     3301 cctgacgaac atctaagaac gcgatcggac cgctaagcag tggactccag aaaggagcac
     3361 gactttgagc aaaccatcga acgccgcaca gcagctaatt ggctcctttg ctccaaagct
     3421 ggttgaactc accgaccggg tgcttttcga cgacgtttgg gagcgcaagg agttgtcgcc
     3481 gcgcgatcgc agcctgatta ccgtagcagc gttgattgcc ctgaaccgcc ccgaccagct
     3541 ccgctttcac ctgggcaagg ccgtcgagaa tggtgtgcaa gagcaggaat tgatcgagtc
     3601 catcacacat ctggcttttt acgcgggttg gcccgccgca atgagcgccg tcatggtcgc
     3661 aaaggaagtg ttttcgaaac gctgaggacc attccacacg aagcaacttg cgatggcgat
     3721 aacatggaac gacctcgcac ctgctgcatg tcggacgagg agggcgtatg ccggcaaaag
     3781 actctctgaa agaccgcctc tcgcgatatc gccaaatcac aatcacagtg accggacgga
     3841 agtcgggacg cgccatttcc atccccgtct ggttcgtcat ggacgacaag ctctacctcc
     3901 tgcccgtcga gggatccaat acgcagtggt acaagaatgt gcaaaagaat cctgtatttc
     3961 ggatcgaggc gcgcggcgca gaagaggaat tccgcgccgt tcccatcacc gatgccgcgc
     4021 aggtctcgtc cgtggtcgaa aaattccgca agaagtatgg cgccggcgac gtgaagaagt
     4081 actattcgaa gttcgacgtt gccgtcctcg cggattccat gaaggctgag taatcaagag
     4141 acaagggcgt ttgctacagt ccacctgatt catcatgagg aaacgaaatg aaaaaacgca
     4201 cacttggaaa cagcaatctt gaggtctcag aaatcgggct cggctgcatg ggaatgagct
     4261 atggcttcgg tccagccggg gacaagcagg agatgattgc gctcattcga tccgccttcg
     4321 aacgcggcgt gacattcttc gacactgctg aggcgtacgg tccattcaag aacgaagagc
     4381 tcgtgggcga ggcgctggcg cccttccgcg accaagtggt gatcgccaca aagttcgggt
     4441 tcaaaatcga ttctaaggcg gagcgagccg ttaacggcgt ggacagccgg cccgagcaca
     4501 tcaggcaagt cgccgaggcc tcgcttaagc ggctcaggac cgatgtcatc gatttgttct
     4561 atcagcatcg tgtcgactcc gacgtgccca tagaagatgt cgcgggaacg gtgaaagagc
     4621 tgatccagca aggcaaggta aaacacttcg gtctttcgga agccggagtg cggacgatcc
     4681 gtcgcgcgca cgcggttcaa cccgtcaccg cactgcagag cgaatactcg ctgtggtgga
     4741 gagagcccga agcggaggtg ctgccggcgc tcgaagaact tggcatcggc ttcgttccct
     4801 tcagccctct tggcaaaggc ttcctcaccg ggcgcatcag ccaggacaca aagtttgaca
     4861 agaacgattt ccgcaacatc gtgccgcgct tcacaccgga gaatcgcaaa gccaatcagg
     4921 cgctggtgga tgtgcttggg cgatttgcag cgcagaagaa agtgacgcct gcgcagcttg
     4981 cactggcctg gctgctggcg caaaagccat ggatcgtgcc gattccgggc acgaccaagc
     5041 tgcatcggct cgaggaaaac atcgcggccg caaacataga actatcgcaa ggtgatctcc
     5101 gccaaatcga cctcgctgct tccaagatca gcgtgcatgg agcgcggtac ccggaagagt
     5161 tgcaaaagct ggtgggccgc tgaacaagcc ccggtgctgc accagtctga gatacgactg
     5221 tcgccgaggt catcccgaag gcccgcgctt tctctaccgg gccgagggat ctcgcgtgca
     5281 cacgctgaca gtcctcgcga gatccttcgc tccgcctgaa gaacggcttc gctcaggatg
     5341 actccatcga aacgcgaaca catagcattc aggccacgtg cgcagtgcat agacgttctc
     5401 ccgcctcgct ccctataatg aaaagattca atcatttggg agacgtgcac gagtgccgtt
     5461 cgagctgaag aacacgatca acctgcccaa gaccgccttt cccatgaagg cgaacctacc
     5521 ccagaacgag cccaaaatgc tcgaacgctg gaagcagatg ggcatttacg agcgcatccg
     5581 acaggcgcgt agaggcgccc agcgctacat cctgcacgac gggcctcctt acaccagcgg
     5641 gccgatacat ctgggcacaa cgatgaacaa gtgcctgaag gatttcgtcg tcaagtcgaa
     5701 gacgatgtcg ggctttgacg ctccctatgt cccgggctgg gactgccatg gcttgcccat
     5761 cgagatcaag gtcgacaagg agctcggcgg caagaagctg cagatgcggc cagccgacgt
     5821 gcgggctgtg tgccggaagt acgcgcagaa atatctcgac ctgcagcgca tgcagttcca
     5881 gcgtatcggc gtgttcgggc gcttcgaccg tccttacgcc accatgaacc cgcagtatga
     5941 gtcggtggtg ctatcgacct tcttttcgtt ctacgagaat gacttcgtct acaaaggcct
     6001 gcgcgcggtt tactggtgca tgcacgatga gaccgcgctg gccgaagccg aagtcgaata
     6061 tgagaatcac accagcccga cggtattcgt gaaatacgcc ctgctcgacg atccggcgcg
     6121 gatcgatccg gcccttgcgg ggaagaaagt atcgaccatc atctggacga cgaccccttg
     6181 gaccctgccg gcgtcgatgg ccgtagcgtt tcatccggat gaggagtatg ttgcgctgca
     6241 gtcaggggct cctgggaaag acgataaatt gtggggcatc gacaacactg aagtgtttat
     6301 cgtcgcgtcg aagctggctg cggatgtcgc ctcaaaatgc ggtctgacgt acccgcacga
     6361 acttgctcat ttccccggcc gcaagctgga gcgcctgaac tttcagcacc cgttcctgga
     6421 ccgcaaaatc ctgggtgtgc tggccgacta cgtaaccatg gacaccggca cgggcgtcgt
     6481 ccatacggcc ccatcgcacg gtgccgaaga cttcgccacc ggcgtcaaat acggcctcga
     6541 cgccacgagc aacgtcgacg aaaaaggcat cctgcgcaac ggcctgcctg aatacaccgg
     6601 caagcgagtc tgggacgcga accagcccat catcgatctg ctcaagtcgc gcggagcgct
     6661 gctgcacacc gaaaagactg agcactcgta tccgcactgc tggcgctgcc acaatcctgt
     6721 gatcttccgc gccacggagc agtggtttat ttcaatggaa acgcccatgc cgggcggagc
     6781 gggcaagaac gacacttttc gcacccgcac gctcgaagag attacgaaag tgaagtggga
     6841 cccggcctgg ggcgaagagc gcctctcgaa catgatccag acccggcccg actggtgcat
     6901 ctcgcggcag cgggtgtggg gcgtgccgat cgctgtgttc ctctgcgaag cctgcggcaa
     6961 gccgctcaac gataaggcga tcaaccgtaa ggtcgtcgag ttgttcgcga agtcgggtgc
     7021 tgacgtctgg ttcacctccg agcccgactc acttgttccc gtcggaatga aatgcccgca
     7081 ctgcgggggc gcgaagttcg aaaaggaaac cgacatcttc gatgtttggc tggagtcggg
     7141 ggccagctat ctcgctctga ttgacgacga accggaatat ccctggccct ccgacctcta
     7201 tctcgaaggc ggcgaccagt accgcggatg gtttcagtct tccctgctgt gcgccatggg
     7261 cacgcaccag acgccgccgt atagaaacgt cgttaccccc ggctggacgc tcgacgaaaa
     7321 aggccaggcc ctttccaagt cgctcggcaa cgccgtcgat ccagtcgaca ttgccggacg
     7381 gctgggcgga gaagtcgtgc gtctgtgggt agcctcggtg gacttccgcg aagatgttgt
     7441 cgcctcggaa acactgatgc agcgcgtcgc ggagaactac cgctcgatcc gcaataactt
     7501 gttcaagaac gcgctcggca atctctacga tttcgatccg caggcgaacg ccctgtccta
     7561 cgaaaagctg gcaaccctcg accagtacat gctccgcgag acatgggctc tcacgcgcga
     7621 ggttcgcaaa tggtatgacg agtttgcttt ccacaagatc tatcaccgcg tgaatcactt
     7681 ctgcgtggtc gatctcagcg ctttttattt cgacgtcatc aaggaccggc tttacacgta
     7741 cgccccgaat tctgctgggc ggcgctcggc gcagacggcg gtctggcgga tctgtgaggc
     7801 gatggtgcgt ttgctcgcgc ccatcctgac ctttacgtgc gaggaggtat ggcagcacct
     7861 gccgaaaatg ccggatcgtg cggagagcgt gcatctcgct gcttttccgg aaattggcga
     7921 cgtgctgggc ccggcttcag ttccagccga agacacgcag cagacgaaag actgggaaac
     7981 cctgctttca gtgcgcgagc aggcgcgcca tcgcctcgca aatccgccac accgccgtct
     8041 gcgccgagcg ccgcccagca gaattcgggg cgtacgtgta aagccggtcc ttgatgacgt
     8101 cgaaataaaa agcgctgaga tccaccacgc agaagtgatt cacgcggtga tagatcttgt
     8161 gaaaggcgaa ctcgtcatac cactggcgaa catcgcgcgt cacagcccag gtctggcgca
     8221 gcatgtactg gtcgagcgct tccagcttct cgtgcgcgac cgcgttcgcc tgcggatcga
     8281 agtcgtagag attgccgagc gcgttcttga acaagttatt gcggatcgag cggtagttct
     8341 ccgcgacgcg ctgcatcagt gtttccgagg cgacaacatc ttcgcggaag tccaccgag
//
LOCUS       DASPUL010000402         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_214892, whole genome shotgun sequence.
ACCESSION   DASPUL010000402 DASPUL010000000
VERSION     DASPUL010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214892"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VEJ00_09775"
     CDS             complement(<1..577)
                     /locus_tag="VEJ00_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01490.1"
                     /translation="MNCVELQKSLAETEAGNSAEQKAHLKTCAECSALVAELNLIASS
                     AAELRATDEPSPRVWNSIEIALRKEGLIRPQRVSRSLLPALRSPWGWARWLAPAAAAL
                     LIVVGLYVRQQSLPAQLAKHAAQPAVAVPSADVELAGLDDADLLQEIAQQTPALKAQY
                     EENLRRVNEYIRDAERDVADNPNDDEARRSLM"
     gene            complement(707..1312)
                     /locus_tag="VEJ00_09780"
     CDS             complement(707..1312)
                     /locus_tag="VEJ00_09780"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXX01491.1"
                     /translation="MTDPAKRQGNELNEAEAINRAQKGDAEAFQALYDRHRRRVYSLC
                     LRMTANTAEAEDLTQEAFLQLFRKIGTFRGESAFSTWLHRLSVNVVLMHLRKKSLPAV
                     SLEETTQGGEEDTPKKDFGAEDLALAGSIDRLQLQKAVEDLPPGYRTIFVLHDVEGYE
                     HNEIAEIVGCSIGNSKSQLHKARMKLRDLLKLNRAEKATKH"
     gene            complement(1574..2854)
                     /locus_tag="VEJ00_09785"
     CDS             complement(1574..2854)
                     /locus_tag="VEJ00_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX01492.1"
                     /translation="MHANTRPEPSRLYRWMVLVFLSLAMFGSYYAYDALSPLADVLKK
                     QLGFSDLNIGFLQMMYSFPNIFTVVIGGFIIDRLGLRKALMIFAVLSFVGPAITVASG
                     QLAIMATGRLIFGMGAESLNVAVTAALARWFKGKELSFAFGLNLTICRLGTFAALNSP
                     TWARAAYANWRWPFLIALGLSVFCVVGAIVYWIMEVNAEKNYHLGEASTDKVVFADLF
                     KFGLSYWYIVALCVTFYSAIFPFQTFAVKFFIEAHGTSREFGGFLSSMLTLFAMIATP
                     LFGLWVDRVGKRAFLMMLGSMLLIPVYLMMAYTRVTLYVPMAMMGIAFSLIPAVMWPS
                     VAYIVDQSKLGTAYGLMTMIQNIGFGGFNLLVGWANTHSHAGPENPRGYDLGMWIFSV
                     LGFLGVLFAFLLHEREIGPRGHGLETITTAKPAT"
     gene            complement(2932..>3174)
                     /locus_tag="VEJ00_09790"
     CDS             complement(2932..>3174)
                     /locus_tag="VEJ00_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01493.1"
                     /translation="PHITLCGRNSVQIHERHRPIDFEEMDRRLRPHGSVRHNDFVLKF
                     WHEPYEMTLFPDGRAIIKGTTDAAIARSLYARYVGS"
BASE COUNT          657 a          949 c          902 g          666 t
ORIGIN      
        1 ccatcagcga gcggcgggct tcatcgtcgt tcggattgtc cgccacgtct ctctccgcgt
       61 cccgaatgta ttcgttcaca cggcgaaggt tttcctcgta ttgcgctttg agcgccggcg
      121 tctgctgggc gatctcctgc agcagatcgg cgtcgtccag acccgccagt tcgacgtctg
      181 cagacggaac ggccacggca ggctgcgccg catgtttggc cagttgcgcg ggcagcgatt
      241 gttgtcgcac gtaaagccca accacgatca gcagggccgc cgccgcggga gcgagccagc
      301 gcgcccatcc ccaggggctg cgcagggcag gcagcaggga acggctgacc cgctgcggac
      361 ggatcagccc ttccttgcgc aaagcgattt cgatggagtt ccacactcgg ggactgggtt
      421 cgtccgtcgc ccgcagctcc gccgccgagg aggcaatcaa atttagctcc gcgaccagag
      481 ccgagcactc cgcgcaggtt ttcaggtgag ccttctgttc ggcgctgttg ccggcttcgg
      541 tctccgccag gctcttctgc agctcaacgc aattcatact gctctcccca agcctctgct
      601 gaaccagtgt accgccgttc tcagcgagct tgccgccgtc tgccgcctct gccgggttac
      661 ttttggccag tctgcgctgg cttgtcggac tttgtcacgc tttcgctcaa tgcttcgtgg
      721 ccttttcggc tctgttcagc ttgagcaggt cccgcagctt catgcgggcc ttgtgcagtt
      781 gcgacttgct gttgccgatg gaacaaccca cgatctcggc aatctcgttg tgttcgtacc
      841 cttccacgtc gtggagcacg aaaatcgtgc gatagccggg gggcaaatct tccaccgctt
      901 tctgcaactg caaacggtca atcgatccag ccagcgccag gtcttccgcc ccgaaatctt
      961 tcttcggggt gtcctcctcg ccgccctgcg tcgtctcctc cagcgagacc gccggcaggc
     1021 tcttcttgcg caaatgcatc agcacgacat tgaccgaaag ccgatgcagc caggtcgaaa
     1081 acgccgattc gccgcgaaac gtcccgatct tgcggaacaa ctgcagaaac gcttcctgcg
     1141 tcagatcctc ggcttccgcc gtattcgccg tcatgcgcag acaaagggag tacacccgac
     1201 gccgatgcct gtcgtaaagc gcctggaatg cttcggcatc ccctttctgc gctctgttga
     1261 tggcctcggc ttcattgagc tcgttgccct gtcgctttgc tgggtcggtc aagttgcctt
     1321 ttccggccgc aatccgtcag atgcggctaa atatctttac gttgtatggc ttgcagtcga
     1381 tcgcgatggc accaaagtaa cccgcatatc cgaaatatta gagctgttct acgtatcttg
     1441 ggattcgcgc tcgcgccatg tttcggtgag aaatcctttg cctgctcagg gttgaaccaa
     1501 acctcttttc aaggtctaac ccgataccga atttagagtc gcaatcttcg gaaatcggta
     1561 aggttccggc ctcctacgtg gcaggtttgg cggtggtaat cgtctccaac ccgtgccccc
     1621 gagggccaat ctcgcgttcg tgtagcagaa aggcaaacag tacgccgaga aacccgagga
     1681 cggagaatat ccacataccc aggtcgtaac cccttggatt ttcgggaccg gcgtggctgt
     1741 gagtattggc ccagccgacc aacagattga atccgccaaa gccaatgttc tggatcatcg
     1801 tcatcagccc ataagctgtc cccagcttgg attggtcaac gatgtaagcg accgaaggcc
     1861 acatcactgc aggaatcagc gaaaatgcta tccccatcat cgccatgggt acatacaaag
     1921 tgacacgagt gtaggccatc atcaggtaaa ccgggatcaa gagcatggac ccgagcatca
     1981 ttaggaacgc acgtttgccc actcgatcaa cccagagtcc gaacagtgga gttgcaatca
     2041 tggcgaaaag cgtcagcata ctcgacagga atccgccgaa ttcgcgagat gttccatgcg
     2101 cttcgatgaa gaacttcacg gcgaacgtct gaaatgggaa gatggccgag tagaacgtca
     2161 cgcacagggc gacgatatac caatagctca ggccgaactt gaacaggtcg gcaaagacga
     2221 ctttgtccgt cgaagcctcg ccgaggtgat agttcttttc cgcattcacc tccatgatcc
     2281 agtaaacgat ggcccccacc acgcagaaga ccgagagtcc cagagcaatc aggaaaggcc
     2341 atcgccagtt ggcgtaggcc gcgcgtgccc aagtcgggga gttgagggcg gcgaacgttc
     2401 ccaggcggca gattgtgagg ttgaggccga acgcgaaact caactccttg cctttgaacc
     2461 atctggcgag cgcggccgtg accgcgacat tcaaggattc ggcgcccatg ccgaagatga
     2521 gccgtcccgt ggccatgatc gctaattgtc ccgatgcgac agtaatcgct ggaccaacga
     2581 aagaaagtac cgcaaagatc atcagcgctt ttcgcaggcc gagccggtcg atgatgaagc
     2641 ccccgatcac gaccgtaaag atgttgggga agctgtacat catctgcagg aatccgatgt
     2701 tcaagtcaga aaagccgagc tgtttcttca acacgtcggc cagcgggctg agcgcgtcat
     2761 acgcgtagta gctgccgaac atggccaggc tgaggaagac cagcaccatc cagcggtaca
     2821 ggcggctggg ctcaggacga gtgtttgcgt gcattcaggc ccttttcgag cggattggac
     2881 tccgcgattg cgaatacagg agattagcag agagaagctg gtagctggaa gctagcttcc
     2941 cacataccgg gcgtacaggc tgcgtgcgat tgccgcgtcg gtggtgcctt tgatgatggc
     3001 gcggccgtcg gggaacagcg tcatctcgta aggctcatgc cagaacttga gcacgaaatc
     3061 gttgtggcgc acgctgccgt gcgggcgcaa tcgccggtcc atttcctcga aatcgattgg
     3121 ccggtggcgc tcgtggattt gcaccgagtt cctgccgcac agcgtgatat gggg
//
LOCUS       DASPUL010000403         4932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_623694, whole genome shotgun sequence.
ACCESSION   DASPUL010000403 DASPUL010000000
VERSION     DASPUL010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623694"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..663
                     /locus_tag="VEJ00_09795"
     CDS             439..663
                     /locus_tag="VEJ00_09795"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HXX01494.1"
                     /translation="MADIGKLLIWLGILMVAAGAALVVLGRLHLPLGRLPGDIVYRGK
                     NTTVYFPLATCVLLSVVLTIVLYVVGRWKK"
     regulatory      863..1085
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1112..3328
                     /locus_tag="VEJ00_09800"
     CDS             1112..3328
                     /locus_tag="VEJ00_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXX01495.1"
                     /translation="MRRLGFALIVAALSAAAFAADLKIKVVDPQGAAVAGAQVELYAR
                     DSKEILAVQTTSAEGYTTFHLATGERCQVRALALGFAAERSDVGNGPELTIRLRLATA
                     SETVVVSATRTPVPGEEAGADVATLSGEELQTMNPVASDDAVRFLPGAVVDAPGRRGS
                     LASLFVRGGNSDYGKMIVDGVSITEPGGTAIYFGTLPLYEADRLEFLRGTQSTLYGSD
                     AMTSVMQVWTRTGSTPVPELRLGADTGNYGTENGYASLSGSHGRFDYNVFGNQFNTAG
                     SGPNDDYSNSLEGANLGFAVNPGVSLRLRVREDHSVAGQPGAWNFNGSNVFNVFGTTF
                     VLAPDQGGRARQNNLVASFDLAVKTGSRWTHHFTGFEYHVKNPDVDSGISTQNTPFGP
                     VNTAFASTIDMNRAGFDYQGDYVERDWARTTVGYEFEDENGSVNSPVPPEFASFGHGL
                     RLNQAVYAQQALTLGRLNVVGGGRFAHNTTFGNVGVPRIAAGFQVFRGGHIFSGTRLT
                     GSYALGIKEPRFEETFVASPFQLENLGLKAERNRALEAGFQQNILRRFVFVANYYNNL
                     FHDQIEFITINPQTFVGKFENVQKSLAHGAEVELQSRLTPRLSWNTSYTYTSTQFLVV
                     PPGSSFPNGQGEPFIRRPKHSGTMLLSYLGSRWGGTLSGSFVGRRTDSDFEGFNIDHT
                     PGHFLLDVGGWYKIHPRITAYANVENATDRFYEEVTGYPALRANFRAGMRFRIGGE"
     gene            complement(3443..4135)
                     /locus_tag="VEJ00_09805"
     CDS             complement(3443..4135)
                     /locus_tag="VEJ00_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HXX01496.1"
                     /translation="MPAERKYREEIVRYGRMVHDRGFVAAMDGNLSVRLNKDRILVTP
                     TCVSKGAMRPADMVVVDMEGQRLSGRRNVTSEIGMHMLIYRLRPDVHAIVHAHPPTAT
                     GFAAAGIALTEPLVCEVVMGLGCIPLACYGTPGTSELAQSLEPYVPNYDAILMSNHGV
                     VTYGDTLEHAYMKMETVEHFAQIALVTHLLGRQQPLKDVEIEKLLLARTKYFGAKAAS
                     SMPMPRAPQKVS"
     gene            4312..>4932
                     /locus_tag="VEJ00_09810"
     CDS             4312..>4932
                     /locus_tag="VEJ00_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HXX01497.1"
                     /translation="MLVSDFNYHLREELIAQEPLADRAGSRLLRLDSSTGATSDLFFR
                     DFPGLLRPNDLLVFNNTRVFPARLYGRRGGLRSQKVSPRNPAARDFLRGQIEVLLTRQ
                     VQEEPNEWECLVRPGRKIGVGEHLFFGQQDELQAEVIGRGEFGERRIRFEEPSNFARP
                     GGRGRPPLHEFFAVIERIGHVPLPPYIAREDSARDRERYQTVYARER"
BASE COUNT          981 a         1281 c         1664 g         1006 t
ORIGIN      
        1 ccggtacgtg cattgttggt gtggtaggtg aggatattct gggcgtgcgc tacgaggccg
       61 gatatgcaca agcttgcaac aagaaggatc gcgagccgaa ggatagtgaa gcaacgaact
      121 gagagactgc gagagcgtga cggtgcggcg gctgggtggc gaaagtgcat agctatctcc
      181 tgagacggac tgagcgaggt gcgttctcgc tccgattggg cggacgaaga gggaagggtc
      241 cttacaccga aacgacaagc gacgactaga ccgaacgatc agcgctgttc cgcgtaatat
      301 cccagaacat caaggcctgg ttcaaacgcg atagtacatg gggagatggc tcgcaaccgg
      361 ttgggaggag agatgaaagg ccacgatccc ggttgcctga ctgggcaaaa attatatcct
      421 agttcagggt atttctcgat ggctgatatt gggaagttgc tgatctggct gggaattttg
      481 atggtggcgg cgggggcagc cttggtcgtg ctgggacgat tgcacttgcc gttgggaagg
      541 ctgcctgggg acattgtcta tcgcgggaag aacacgacgg tctattttcc gctggcgacg
      601 tgcgtactgc tgagtgtcgt gctgacgatc gttttgtacg tggtggggcg gtggaagaag
      661 tagggacagg cgtcacagaa gatccttcca ccaaagggca ggaacgaaga tggagcaaaa
      721 ggtcgagctg cgctcgaccg gacagccgag ggcggctgtc cctacatggc atcctgtcgg
      781 ttggcgggcg ttgcgccttg agcatttgac ttccacagag cgctgacagt aaattcgtcc
      841 gacggtcgta cctgttcttt ctcatttcct tcggaacgcg cgtctttgcc gctcggcgaa
      901 gacagggaag cgggtgtgaa tcccgcgctg ccgcgcaact gtaagcgagg acattgcctc
      961 ggccactggg agggatcggt aaagctgatt taccgaatca gttccgggaa ggccgcgggc
     1021 gctgctgggt gttgagcatc cttactcgcg aagccaggag accggcgcga tccgtttacc
     1081 tcaacctctt tcgcgtgaca aggaggatgg tatgcgcaga cttggctttg cgctgatagt
     1141 tgcagctctt tccgcggcgg ccttcgccgc cgatctcaaa atcaaagtgg tcgatccgca
     1201 gggcgcggcg gtcgcgggcg cgcaagtgga gttgtacgcg cgcgactcga aagaaatatt
     1261 ggcggtgcaa accacctcgg ctgagggata tacaaccttc catctcgcaa cgggggagcg
     1321 gtgtcaggtc cgggcactcg cgttgggttt cgctgcggag aggtctgacg ttgggaacgg
     1381 tcctgaactc acgatcaggc tgcggctggc gacggcatcc gaaactgtcg tggtgagcgc
     1441 gacgcgaacg ccggtaccgg gggaagaagc gggagccgac gtggccacct tgagcggcga
     1501 agaactgcaa acgatgaatc cagtagcgtc tgacgacgct gtacggtttc tgccgggagc
     1561 tgtggtggac gcgccgggaa ggcgtggttc attggcatcg ttgttcgtgc gcggaggaaa
     1621 ttcagactac ggaaaaatga tcgttgatgg cgtgagcatc accgagccgg gcggcaccgc
     1681 gatttatttc ggcacgctgc cgttgtacga agcggaccgc ctggaattcc tgcgaggcac
     1741 ccagagcaca ctctacggtt ccgatgccat gaccagcgtc atgcaggtct ggacgcgcac
     1801 gggaagcacg cctgtaccag aattgcggct tggggcggat acgggcaact acggaacaga
     1861 aaacggctac gcgtctctga gcggctcgca tggacgcttc gactacaacg tgttcggcaa
     1921 tcaattcaat accgccggtt ccggccccaa tgacgactac tcgaattccc tggaaggagc
     1981 caacctgggc ttcgcggtga atccgggtgt ctcgctgcgt ctgcgagtgc gcgaggatca
     2041 cagcgtggcg gggcagccgg gggcgtggaa tttcaacgga tcgaacgtat tcaatgtgtt
     2101 cggtacgacg tttgtcctcg cgcccgacca gggcgggcgc gcgcggcaga acaatctggt
     2161 tgcgagtttt gatctggcgg tgaagacagg atccagatgg acgcatcatt tcaccggctt
     2221 cgaataccat gtgaagaatc ccgatgtgga cagcggcatc tcgacgcaga acactccgtt
     2281 tggccccgtc aacacggcct ttgcgtcgac cattgacatg aatcgggccg gctttgacta
     2341 ccagggagac tatgtcgagc gggattgggc gcggactacg gttggctacg agttcgagga
     2401 tgaaaatgga tcggtcaata gccctgtacc acctgaattt gcgagctttg gccacggcct
     2461 gcggctgaac caggctgtct acgcgcagca ggcgctcaca ttgggacggt tgaatgtagt
     2521 aggcggaggg cggtttgcgc acaacacgac ctttggcaat gtcggtgtgc cgcgcatcgc
     2581 ggcgggattt caggtgttcc ggggcgggca cattttctcc ggcacccggc ttacaggatc
     2641 ttacgcgcta ggcatcaagg agccgcgatt tgaagaaaca tttgtggcct ctccgtttca
     2701 gctggagaat ctgggcctga aggcggagcg caaccgggca ttggaagcgg ggtttcagca
     2761 gaatatcctg cgccgcttcg tgttcgtcgc caactattac aacaatctgt tccacgatca
     2821 gatcgaattc atcacgatca atcctcagac tttcgtgggg aagtttgaaa acgtgcagaa
     2881 atccctcgct cacggagcgg aggtggaact gcagagtcga ctgacgccgc gcctctcctg
     2941 gaacacctcc tatacctaca cctccaccca gtttctggtc gtgcctccgg ggagctcttt
     3001 tccgaatggg cagggagaac cgttcatccg ccgtccaaag cactccggga cgatgttact
     3061 gagttatctg ggttcgcgct ggggcgggac cttgagcggc agctttgtcg gacggcgcac
     3121 ggactccgac tttgaagggt tcaacatcga tcacacacca gggcattttc tgctggatgt
     3181 gggcggctgg tacaagatcc atcccaggat aacggcgtat gccaatgtgg agaatgctac
     3241 ggatcgtttc tacgaagagg tgacgggata cccggcgctg cgggcgaact ttcgcgcggg
     3301 tatgcggttt cggattggag gggaataggc aagatgacat cccggcaggg gctaaagccc
     3361 cttgagtcat gagcccaacg ggacggcgcg gctggaagcc gcgccctttc agaactgcgc
     3421 tactgcaaat cagcgctcgc agctagctga ctttttgcgg agcgcggggc atgggcatgg
     3481 aggaggcagc cttcgcgccg aaatacttgg tgcgagccag gagcagcttt tcgatttcca
     3541 cgtctttcaa cggctgctgg cggccgagca ggtgcgtgac cagggcgatc tgggcgaagt
     3601 gctcgacagt ttccatcttc atgtaggcgt gctcgagcgt atcgccgtag gtgacgacgc
     3661 cgtggttcga catgaggatg gcgtcgtagt taggaacgta gggttcgaga ctctgcgcca
     3721 gttctgaagt accgggcgta ccgtagcagg cgagcgggat acatcccagg cccatcacga
     3781 cttcacatac gagaggttcc gtcagcgcga tgccggcggc agcgaagccg gtcgcggtcg
     3841 gaggatgggc gtgaacgatg gcgtgaacgt cggggcgcag gcggtagatc agcatatgca
     3901 tgccaatttc gctcgtgacg ttgcggcgcc cggagagacg ctgaccttcc atatcgacca
     3961 cgaccatgtc ggccgggcgc atggcgcctt tactgacgca ggtcggggtg accaggatgc
     4021 ggtccttgtt cagccggacg gagagattgc cgtccatggc ggcgacgaag ccgcggtcgt
     4081 gcaccatgcg cccgtagcgg acgatttctt cgcgatattt gcgttccgca ggcatgggtg
     4141 ccgtcgggga agtgggcaaa cagcaggccg gaccgcgatt acgggccgaa ctccagcacc
     4201 cgcatagtac cagcagtctg cgacgggaaa caatgccatt ttccggggag atgaagctct
     4261 atcgtgcgat tcacctctgt tcggtgcggc gcgtccctat aatcgggtga cgtgctggtc
     4321 tctgatttca attatcacct tcgggaagaa ttgattgcgc aggagccgct ggcggatcgg
     4381 gcgggctcgc ggctgctgcg gctggattct tcgaccgggg cgacttcgga cctgttcttc
     4441 cgcgactttc ccggcctgct ccgaccgaac gatctgcttg tcttcaacaa cacacgtgtc
     4501 tttccggcgc ggctgtatgg ccggcggggc gggttgaggt cccagaaggt gagcccgagg
     4561 aatccggcgg cacgggattt tcttcgtgga cagattgagg tgctgctcac gcggcaggtg
     4621 caggaagagc cgaatgagtg ggaatgtttg gtgcgtccgg ggcggaagat tggggtaggg
     4681 gaacatctgt tttttggcca gcaggatgag ttgcaggcgg aggtgattgg gagaggcgag
     4741 tttggggaga ggaggattcg gtttgaggaa ccgtcgaact tcgctcgacc gggcggacga
     4801 gggcgtccgc ccctccacga gttttttgcc gtgatcgaga ggatcgggca tgtgccgctg
     4861 ccgccttata ttgcgcggga ggactcggcg cgggatcggg agaggtatca gacggtgtat
     4921 gcgcgggagc gg
//
LOCUS       DASPUL010000404         3333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1169275, whole genome shotgun sequence.
ACCESSION   DASPUL010000404 DASPUL010000000
VERSION     DASPUL010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169275"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..1775
                     /gene="selA"
                     /locus_tag="VEJ00_09815"
     CDS             252..1775
                     /gene="selA"
                     /locus_tag="VEJ00_09815"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HXX01498.1"
                     /translation="MKSTAKSDLFRELPSVDELVRMPGVATLVASSGLAPVTDAARAV
                     LSRLRQEIASGLLDAEALRLALDGVAGAIEAQLREALGYSLRAVINATGVILHTNLGR
                     APLAESALEHIRETAGRYSNLEFEIQAGERGKRDVHVERLLRGLLNEGASFRDDSARL
                     RPRGTGEAPVATQPVATIVVNNNAAAVLLALNTLAEGGEAIVSRGELVEIGGSFRIPD
                     VMAKSGAILREVGTTNRTRIADYEKAINERTRLLLRVHRSNFEITGFTEQPATAELVQ
                     LARKRGLPLMEDLGSGALVDLENFGIRGEPSVLDSLRAGVDVVTYSGDKLLGGPQAGL
                     ISGHADLVTRMRSNSLFRALRVDKLTYAALEGTLLAYVKGEHDAIPALRMMRLTKEEI
                     GKRAEAVAEQVRSGENPHFSRQERARNGAPDRMKIEIVDGESVIGGGAAPSSVLPTRL
                     LAVTCAGMSADDLAAQLRLGDPPIVARVEDGRVLLDLRTVFAEQDALIAAALRRIAA"
     gene            1797..2327
                     /locus_tag="VEJ00_09820"
     CDS             1797..2327
                     /locus_tag="VEJ00_09820"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HXX01499.1"
                     /translation="MATFFTIGHSTRTLDELVVALRAHHIQALVDIRAFPMSRRLPQF
                     NRETLEKDLRAAGIQYVWLKELGGYRKKILEDSPNIALRNDSFRNYADYMLTPEFEQA
                     MGKVVVLARHSPTAYMCAERLYFRCHRMLVSDWLTAHGHEVLHIDGTGPVKRHVLTAE
                     ARTIDGRLVYRGDRLL"
     gene            2498..2875
                     /locus_tag="VEJ00_09825"
     CDS             2498..2875
                     /locus_tag="VEJ00_09825"
                     /inference="COORDINATES: protein motif:HMM:NF011254.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-[acyl-carrier-protein] synthase"
                     /protein_id="HXX01500.1"
                     /translation="MIVGTGIDIAEVPRIRRTIERFADRFLRRIFTAGEMRYCDSKAN
                     RVERYAARFAAKEAAMKALGTGWNYGVRWQDCEVVRMPGGRPTIAFHGKAAEFARKLG
                     VKNAALSLSHTAEQAIAQVILEG"
BASE COUNT          621 a          926 c         1168 g          618 t
ORIGIN      
        1 gagtggcctg cccgcgaatg acgagcgggt ggccgacatc attcgattgg gacggcacga
       61 aatgccggga tacagccaga cgctgagcgc ccaggatatt caggacctgc tggcgtatat
      121 gcacacgctg taggtcggga aggattccca acttctcgcg cagcggacgc tcgagaaatg
      181 gggcacccga gagtcagaac ccaccccctc gcacggaacg cgagaagggg ggcaaccgca
      241 gaaagccttg gatgaagagc acggcaaaat ccgatctctt tcgcgagctg ccgtccgtgg
      301 acgagctggt gcggatgccg ggcgtagcta cgctcgtggc cagttccgga ctagcgccgg
      361 ttactgacgc ggcccgtgcg gtgctgtcgc ggctgcggca ggagatcgct tccggattac
      421 tggatgcgga ggctttgcgg ctggccctgg acggagtggc tggagcgatt gaagcgcagc
      481 ttcgcgaggc gctgggatat tcgctgcgcg cggtgatcaa tgcgactggc gtcatcctgc
      541 atacgaatct ggggcgagcg ccgctggccg agtctgcgct cgagcacatc cgcgaaacgg
      601 cggggcggta ttccaatctc gagtttgaga ttcaagcggg ggagcggggc aagcgcgacg
      661 tgcatgtgga gcggctgcta cgcgggttgt tgaatgaggg cgcgagcttc agggatgatt
      721 ccgctcggct gcgccctcgc gggacgggcg aggcgcccgt cgccacacag cctgtggcga
      781 ccatcgtcgt gaacaacaat gcggcggcgg tgctgcttgc gctcaatacg ctcgccgagg
      841 gcggagaggc cattgtctcg cgcggcgaac tggtggagat tggcgggtcg tttcgcattc
      901 ccgatgtgat ggccaagtcg ggggcgattt tgcgcgaagt gggcacgacc aaccgcacgc
      961 gcatcgcaga ctacgaaaag gcgatcaacg agcgcacgcg gctgctgctg cgcgtgcacc
     1021 ggtcaaattt tgagatcaca ggattcaccg agcagccggc gacggcggaa ctggtgcaac
     1081 tggcgcgcaa gcgcgggctg ccgctgatgg aggatctggg cagcggggcg ctggttgatt
     1141 tggagaattt cgggattcgc ggcgagccca gcgtgctcga cagcctgcgc gccggcgtgg
     1201 acgttgtgac ctacagcggc gacaagctgc tgggtgggcc gcaggcgggt ttgatcagcg
     1261 ggcacgcgga tctggtgacg cggatgcgct ccaactcatt gtttcgggcg ttgcgcgtcg
     1321 ataagctcac ctatgccgcc ctggaaggga cgttgctcgc gtatgtcaag ggcgagcacg
     1381 atgccatccc ggcgctgcgc atgatgcggc taacgaagga agagattggg aaacgcgccg
     1441 aggcggtggc ggagcaggtg cggagcgggg aaaaccccca cttctcgcgc caagaacgcg
     1501 cgagaaatgg ggcacccgac agaatgaaga ttgaaattgt cgatggggag tcggtgattg
     1561 gcggcggggc ggcaccgtcg tcggtcttgc ccacacggtt gctggcagtg acttgcgccg
     1621 ggatgagtgc ggacgatcta gccgcgcagc tgcgtttggg cgatccgccg attgtggccc
     1681 gggtggagga tgggcgagtg ctactcgatc tgcggacggt gtttgcggaa caggatgccc
     1741 tgatcgcggc ggcgctgcga cgcatcgcgg cctagccgca gcgcatcgag atctggttgg
     1801 ctacgttctt caccatcgga cactcgaccc gcacgctcga cgaactggtc gtggcgttgc
     1861 gagcgcacca catccaggct ctcgtcgata ttcgtgcttt cccaatgtcg cgacggctgc
     1921 cgcaattcaa ccgcgagacg ctggaaaaag atctgcgggc ggcggggatc cagtatgtgt
     1981 ggctgaaaga gctcggcggg tatcgcaaaa aaattctcga ggactcgccg aacattgcgt
     2041 tgcgcaacga cagtttccgg aactacgcgg actacatgct tacgcctgag tttgagcagg
     2101 cgatgggcaa ggtcgtcgtg ctggctcggc actctccgac tgcctacatg tgcgccgagc
     2161 ggctatattt tcgctgccat cgcatgctgg tttccgattg gctgacggcg catgggcatg
     2221 aggtgttgca tattgacggg acggggccgg tgaagcggca cgtgctcacg gccgaggcgc
     2281 ggacgatcga cgggcggcta gtctatcggg gggacaggtt actgtagctt tgcgatttta
     2341 tccgaccctg gcgcaaaaag cgcgacaagg gtcgggcaac cgcgttgtca tagaaacacg
     2401 accaccgggg ctaaagcccc gaagatctga ggcgttttac gcggccctga agggccgctc
     2461 ttccacggcg agcccttggg tccgaggatg gaattccatg atcgttggta caggaattga
     2521 cattgccgag gttccgcgga ttcggcggac gatcgagcgg tttgcggacc gctttcttcg
     2581 gcgcatcttt actgcgggcg agatgcgcta ttgtgattcc aaggcgaatc gcgtggagcg
     2641 ctatgcggcg cggtttgccg cgaaagaggc tgcgatgaag gcgctcggga cgggttggaa
     2701 ttatggtgtg cggtggcagg attgcgaagt ggtacgcatg cccggcgggc gtccgacgat
     2761 cgcgtttcat ggcaaagcgg cggagtttgc ccggaagttg ggggtaaaga atgcggcgct
     2821 gtcgctttcg catacagcgg agcaggcgat cgcgcaggtg attctggagg gatagccgac
     2881 ctgacgtgga aagtagagat tccagcctgt caagaacgac cacaagggct aaagcccggt
     2941 cggggtcgag gctagacgcg gcgctgaagc accgctcttc cacggtgctc caaacaccat
     3001 ctcaaccatt tggccatttc cacacggtac tcgagtcagc gtctcgacga tcttccacca
     3061 aacaccgctg tcgcgtcatc ccgacgctcc ggcatcgaat tgtcagcgcc ccgattttcg
     3121 cctggggccg ctttcactgt tgttattgga accgggttcc acaatgggcc cgaaagcggg
     3181 gcggggcccg ggatttgccc tagctggctt gcaacggctg agtggcggcg ctatgattcg
     3241 cctcgaactg gcgccgaaga cagattcctc tccgctccat gccggagcgc cgcactcctc
     3301 gacgcagaac gatcgctggg ggctggcagt gac
//
LOCUS       DASPUL010000405         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1718818, whole genome shotgun sequence.
ACCESSION   DASPUL010000405 DASPUL010000000
VERSION     DASPUL010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1718818"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VEJ00_09830"
     CDS             complement(<1..555)
                     /locus_tag="VEJ00_09830"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01501.1"
                     /translation="MSQRLGDLLVKEKIITPEQLEQATKVQKEQSCRLGSALVKLGFL
                     TDEDVTNFLSRQYGVPAINLSYFEIDPAVVKLIPFETAKRYQILPLSRVGASLTIAMV
                     DPTNVFAMDDIKFMTGFNIEPVVASESSILAGIDKAYGATKEEDLETVMQSMSELSDE
                     DVELQGEQQEMELSELEKAADEAPV"
     gene            1071..1874
                     /locus_tag="VEJ00_09835"
     CDS             1071..1874
                     /locus_tag="VEJ00_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01502.1"
                     /translation="MSRKRAPTVEATKEDELRRYYRTLHEAWGAQHWWPAETDFEVIV
                     GTYLTQNTAWTNVQRALANLSAAGVLSLEGIREVRLSRLERLIRPSGYFRQKARRIKT
                     FIAFLDRQYGGSLERLFAQPTDKLREELLDLNGVGPETADSILLYAGNHPVFVVDAYT
                     RRILDRHGILPENADYEAFRQFCERALAPLAEEPQGAQAGMSSNCESRGAAHPPSAMS
                     TAPRSALAQVYNEMHGLIVGVGKQYCRKSEAACEKCPLRPFLHPEQTEK"
     gene            1957..2301
                     /locus_tag="VEJ00_09840"
     CDS             1957..2301
                     /locus_tag="VEJ00_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01503.1"
                     /translation="MKVVGIDGMSPQQLEFEIQRGAKLVCYQYCISIVVMTFRRSSDI
                     YFIPAGGSAVSKGLPWILLTLVAGWWGIPWGPIFTVQSLVTNFRGGKDLTAEFRARMS
                     QPAAAPLVTAKA"
     gene            2309..>2965
                     /locus_tag="VEJ00_09845"
     CDS             2309..>2965
                     /locus_tag="VEJ00_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HXX01504.1"
                     /translation="MQFPACFRLASQLCAIAALVSSSSPTLREAANSTGILVGTAVRP
                     YALSEPAYTETLAREFNMVEPEDALKWWVVRKTQDFDFREGDAVVRFAQAHAMKVRGH
                     CLVWDHTNPDWLVQGHFTSEQMSRLLREHITTVMKHYAGQVFAWDVVNEALDENGRVK
                     DSPWYNLPGIGFAGQGPAYIEQAFRWAREADPHALLFYNEGGGAETLNRKSDTMYAMV
                     R"
BASE COUNT          588 a          853 c          897 g          627 t
ORIGIN      
        1 gacgggagct tcgtcggcag ccttttccag ctccgacagc tccatctcct gttgttctcc
       61 ctgcaattcg acgtcttcgt cgctcagctc cgacatcgac tgcattacgg tttcgagatc
      121 ttcttccttg gtcgcgccgt aagccttgtc gatccccgcc agaatcgagc tctcggaggc
      181 cactaccggc tcgatgttga agccggtcat gaacttgatg tcgtccatgg cgaagacgtt
      241 cgtgggatcg accatggcga tggtgagcga cgcgcctacg cggctcaagg gaaggatctg
      301 gtaccgcttg gcggtctcaa aggggatcag cttgacgacc gcggggtcga tttcaaaata
      361 ggaaaggtta atggcgggaa cgccatactg ccgggagaga aagtttgtga cgtcctcgtc
      421 cgtgaggaag ccgagcttca ccagggcgga ccccaggcgg cagctctgct ccttctggac
      481 cttggtcgct tgttccaact gctcgggagt tatgatcttc tctttgacga gaaggtcacc
      541 cagccgttga gacatacgtc gatctcctat ccacgtagat ggaacgacgc ctggcgcttg
      601 gacaggcggt gacgcagatg gctggtaatc gttgggagag aaacgcagcg cattcgcgtt
      661 gacataaatt gtcaacgcaa gcgggcgacg gtaccactta ctttggtact tgcacctgaa
      721 agcgacagcg ggtcggcggc gcctcgtgct gcggaactcc gcgccaaacg cctgtttttc
      781 gctacggata gaagccgcgc cggctcaagt acatgcactt cagtaaggct agcgtgggcg
      841 ttcccgggca acggcagagc ctgcggattc tccgtcgagg ctggccgttt gtcttctttg
      901 cgctttttgc ggcattcctt tgcggtcttt gcgtttcaca gctttgaccg ccaagttcgc
      961 aaaggaatgg cccgcaaagc acgcgaagaa caccggcgct gtggtttcgc gagtcaaact
     1021 gatggctgat tcaagatgca tcaatatcgc aagcttgagg aaatcttaac gtgtcgcgaa
     1081 aaagggctcc caccgtcgag gctacgaaag aagacgaact gcgccggtac tatcgcaccc
     1141 tgcatgaggc ctggggagcg cagcactggt ggccggccga aactgatttt gaggtcattg
     1201 tcggaactta cctgacccag aatacggcgt ggaccaatgt tcaacgcgcg ctggcgaacc
     1261 tgagtgcggc aggagtcctc agcctggaag gaatccgaga agtcagactc tccaggctgg
     1321 agcgcctgat tcggccttcc ggctacttcc gtcagaaggc gcggcggatc aagacattta
     1381 tcgccttcct cgaccggcag tacggcggct cgctcgagcg gctgtttgcc cagcccaccg
     1441 acaagctccg tgaggaattg cttgacctga acggcgtggg cccggagacg gccgattcaa
     1501 tcctgcttta tgcgggcaat cacccggtct tcgtcgtcga tgcgtacaca cggcgcattc
     1561 tcgatcgaca tggaattctg ccggagaacg ctgactatga ggcctttcgc cagttctgcg
     1621 agagagcttt ggccccgctc gcagaagagc cgcagggtgc gcaagcgggt atgtcgagta
     1681 actgcgaatc tcgcggtgcg gcccacccgc cttcggctat gagcacggcg ccgcgatcgg
     1741 cccttgccca ggtctataac gagatgcacg gactgatcgt cggagttggc aagcagtact
     1801 gcaggaaatc cgaggctgcc tgtgagaagt gtcccttgcg gccgtttctg catcctgagc
     1861 agaccgagaa gtagtctcca ggaggacagt acactttctg ctgctttacc gggcattatc
     1921 cccggcctac aattcgccat cccccaagga gaaatcatga aggttgtagg catcgacggg
     1981 atgtctcctc agcagctcga attcgagatt cagcggggag cgaagctggt ctgctaccag
     2041 tactgcattt cgattgtggt catgaccttc cgccgttcct cggacatata cttcattccc
     2101 gcgggaggaa gcgctgtatc caagggtttg ccctggattc tgctcactct ggtcgcaggc
     2161 tggtggggaa ttccctgggg accgattttc acggtgcagt cgctggtgac gaatttcaga
     2221 gggggaaagg atctcaccgc cgagttcagg gcacgcatgt cgcagccggc tgcggcaccc
     2281 ctggtcaccg caaaagcctg agggctggat gcagtttcct gcttgtttcc gcctcgcgag
     2341 ccaactctgt gcgatagcag ctctcgtttc ttcgagttcc ccaacgctca gagaggccgc
     2401 gaattctacc ggcatcctgg tgggcacggc ggtgcgtccc tatgcgttgt ccgagcccgc
     2461 ctacacagaa acgctcgctc gtgaattcaa catggtcgag cctgaggacg cgctgaagtg
     2521 gtgggttgtg cgcaagaccc aggacttcga tttccgcgaa ggcgatgcgg tcgtgcgctt
     2581 tgcacaggcc cacgccatga aagttcgcgg gcactgcctc gtatgggatc acacaaatcc
     2641 tgactggctc gtgcagggcc acttcacatc cgagcagatg tcccgcctgc tgcgggaaca
     2701 catcacgact gtcatgaagc actatgccgg ccaggttttt gcctgggacg tggtgaatga
     2761 agcgctggat gagaacggcc gcgtcaaaga ctccccgtgg tacaacctgc cgggcatcgg
     2821 attcgcgggc caagggccag cctacatcga gcaggccttt cgctgggcgc gggaggccga
     2881 cccgcacgcg ctgctcttct acaacgaggg tggtggagcg gagacgctga accgaaaatc
     2941 ggacacgatg tatgccatgg tgcgg
//
LOCUS       DASPUL010000406         3862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1169429, whole genome shotgun sequence.
ACCESSION   DASPUL010000406 DASPUL010000000
VERSION     DASPUL010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169429"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /gene="queA"
                     /locus_tag="VEJ00_09850"
     CDS             <1..708
                     /gene="queA"
                     /locus_tag="VEJ00_09850"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HXX01505.1"
                     /translation="GGRGRPPLHEFFAVIERIGHVPLPPYIAREDSARDRERYQTVYA
                     RERGSMAAPTAGLHFTPEILARIRECGVETVEITLHVGLGTFQPVHVERVEEHKLHSE
                     VYSISEEAAEKINRARSAGRRVVAVGTTTVRTLEYAARPIGIPHFSRTERARNGAPEV
                     RAGRGEADLFIYPGYKFQVVGALLTNFHLPQSTLLMLVCALGGKENVLRAYEHAVAQR
                     YRFYSYGDCMFVESQQQ"
     gene            905..3382
                     /locus_tag="VEJ00_09855"
     CDS             905..3382
                     /locus_tag="VEJ00_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXX01506.1"
                     /translation="MHSVSPLRFVAVAALAVPVTWFGLQAQTNLKYQEPPKAIVDLVD
                     TKPTPAVEVSPKDKAGRQWLLIGTISGLPPIADLAQPELRLAGLRFNPKTNGPSRGRY
                     FTSLRLQALPDGKEKAVSGLPANAKIRFTAWAPDARHVAFVNISDTRDAGLSLWIVDV
                     ATAQAKQLPGVALNGVFGPPCEWMSDSQSLTCKTMVKGIRAAPLRSEVPTGPVIQENL
                     GRVTPGATYEDLIKSPEDERIFDYYATSQVEVVRLDGTMKLVGKPGVIQGASPSPDGK
                     YVMIDERHHPYSYVLPLEFFPERVSVVNLATGASKQLVDQPLEDTIPNIHDAVEAGPR
                     EFDWRSDAAATAFWVEAGDGGDPRKDVAIRDTLFLEDAPFDGAPRKLAELPVRFRSVV
                     WGNGKLALIEEERWKDRKRIMLAVAPDGGAPVKLFEGSFQDRYHDPGRPLEVMNASGK
                     SVLETTTDGTGIYLRAEGASPEGDRPFLATMNATTGESKRLWQSEDKFYEFPAAVLES
                     EGPCVLIRRESPELPPNYYIKDPAKDDVKQVTFFPNPYGDAPLPKKQVLKYKRADGVD
                     LSANLYLPPGYKPEDGPLPALMEAYPTEYKTKAATGQISGSPYEFPLFYWGSPIPFVT
                     QGYAVLENATIPIVGEGNAEPNDTYVEQLVASAQAAIGEGARLGVVDKNRVAVMGHSY
                     GAFMTANLLAHCDLFKAGIARSGAYNRTLTPFGFQNEERTYWQAPEIYYKMSPFSYAD
                     KIKTPVLLIHGEADNNSGTFPIQSERLFSAIKGAGGTVRFVLLPLESHGYAGRESVLH
                     MFWEMNNWLDTYVKNPRAATAAKQAAN"
     gene            complement(3424..>3862)
                     /locus_tag="VEJ00_09860"
     CDS             complement(3424..>3862)
                     /locus_tag="VEJ00_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01507.1"
                     /translation="IDLFDPKTRQISQLSGSTGFFGPRWSPDGRYIVALAHDGNEMML
                     FDVKKQTWRQLDINLNSFGYLAWSRDSAYVYFDTILTNGTGYYRVRITDAKLEKVADL
                     KKIRGFPSQFGPGSWTGLGPGDTFLFPRDISTQEIYSFDLQLP"
BASE COUNT          764 a          996 c         1360 g          742 t
ORIGIN      
        1 ggcggacgag ggcgtccgcc cctccacgag ttttttgccg tgatcgagag gatcgggcat
       61 gtgccgctgc cgccttatat tgcgcgggag gactcggcgc gggatcggga gaggtatcag
      121 acggtgtatg cgcgggagcg gggatcgatg gcggcgccga cggcggggct gcattttacg
      181 ccggagattc tcgcgcggat tcgcgagtgc ggggtcgaga ccgtggagat cacgctgcac
      241 gtcgggctgg ggacgtttca gcccgtgcat gtggagcggg tggaagagca caagctgcat
      301 tcggaggtgt attcgatttc ggaggaggcg gcggagaaga tcaatcgggc gcggagcgct
      361 gggcggcgcg tggttgcggt ggggacgacg acagtgcgga cgttggagta tgcagcgcgg
      421 ccgatcggaa ttccccactt ctcgcgcaca gaacgcgcga gaaatggggc acccgaggtg
      481 agggcgggca ggggcgaggc ggatttgttc atttatcccg ggtacaagtt tcaggtggtg
      541 ggagcgctgt tgacgaactt tcatctgccg cagtcgactc tgttgatgct ggtgtgcgcg
      601 ctgggcggca aggagaacgt tttgcgagcg tacgagcatg cggtggcgca gcggtatcgg
      661 ttttattcgt atggggattg catgtttgtg gagtcgcagc agcaatgacg accaccgggg
      721 ctgaagcccc ttctccattt gccgcttacg cggcgctgaa gcgccgctct tccacggcga
      781 gccctgacgt ccacggtgag ctgacatccc tggtaagcgc tggtgttctg tgtctcccat
      841 gactgccacg ctagcgaccc ttccgcggta gtattgaagc ctcattcgac aaggagtatt
      901 tcttatgcac tctgtttccc ccctcagatt tgtggccgtg gctgcgctgg ccgttcctgt
      961 gacttggttc ggcctacagg cccagaccaa tctcaagtat caggagcctc cgaaggcgat
     1021 cgtcgatctg gtggacacga agccgactcc ggcggtggaa gtttcgccga aggacaaggc
     1081 gggcaggcag tggctgctga ttggaacgat ttcggggttg cctccgatag cggatctggc
     1141 gcagccggaa ctgcggctgg cggggttgcg gttcaatccg aagacgaacg ggcccagccg
     1201 cgggcggtat ttcacttcgc tgcggctgca ggctttgcca gacggcaagg agaaggccgt
     1261 ttcagggttg ccggcgaacg cgaagatccg gttcacagcc tgggcaccgg atgcacgaca
     1321 tgttgcgttc gtgaatatca gcgacaccag ggatgcgggc ttgagcttgt ggattgtcga
     1381 tgtggcgacg gcgcaggcga agcaattgcc gggcgtcgcg ctgaacgggg tgtttggtcc
     1441 gccttgcgaa tggatgagcg acagccagag cctgacctgt aagacgatgg tcaaaggcat
     1501 ccgggcggct ccgctgcgga gcgaagtgcc gaccgggccg gtcattcagg aaaatctggg
     1561 gcgggtgacg ccgggcgcga cttatgaaga tttgatcaag agtcctgaag acgagcggat
     1621 tttcgattac tacgccactt cgcaggtgga agtggttcgg ctcgacggca cgatgaaatt
     1681 ggtcggcaag ccgggagtga ttcagggcgc ctcgccttcg cccgatggaa agtacgtgat
     1741 gatcgacgag cggcatcatc cgtattcgta tgtgctgccg ctggagttct ttccggagcg
     1801 ggtttcggtc gtgaacctgg cgaccggggc gagcaaacag ctcgtggatc agccgctcga
     1861 ggacacgatt ccgaacattc acgatgcggt cgaagccggg ccgcgggaat ttgactggcg
     1921 tagcgatgcg gcagcgaccg ctttttgggt ggaggcgggc gatggcggcg atccgagaaa
     1981 ggatgtcgcg attcgcgaca cacttttcct tgaggacgcg ccgtttgacg gagcaccgcg
     2041 caagctggcg gagcttccgg tgcggtttcg cagcgttgtg tggggaaacg ggaagctggc
     2101 tctgatcgaa gaagaacgct ggaaagaccg gaagcggatc atgctggcgg ttgctccgga
     2161 cggcggcgcg ccggtgaagt tgtttgaagg atcgtttcag gaccggtatc acgatccggg
     2221 gcgcccgctc gaggtgatga acgcgagtgg gaagagcgtg ctggagacga cgacggacgg
     2281 cacgggaatt tacttacgtg cagagggcgc gtcaccggag ggagaccgtc cgtttctggc
     2341 aacgatgaat gcgacgactg gagaatccaa gcggctatgg cagtcggagg acaagttcta
     2401 tgaattcccg gcggcggtgc ttgagagtga ggggccgtgc gtgctgattc gcagggaatc
     2461 gcccgagctg ccgccgaatt attacatcaa ggatccggcg aaggacgatg tgaagcaggt
     2521 aacgtttttt ccaaatcctt atggcgacgc gccgcttccc aagaagcagg tgctcaagta
     2581 caagcgtgcc gatggagtgg atttgagcgc gaatctgtat ttgccgccgg gatacaagcc
     2641 ggaagacggg ccgctgccgg cgctgatgga agcgtatccg acggagtaca agacgaaagc
     2701 ggcgacgggg cagatcagcg gatcgccgta cgagtttccc ctgttttact ggggttcgcc
     2761 gataccgttc gtgacgcagg ggtacgcggt gctggagaac gccacgattc cgatcgtggg
     2821 agaagggaat gcggagccga acgataccta tgtcgaacaa ctggtcgcga gcgcgcaggc
     2881 ggccattggc gagggcgcgc ggctgggagt ggtcgataag aatcgcgttg cagtgatggg
     2941 gcactcgtat ggagctttca tgacagccaa cctgctggcg cactgcgatc tgttcaaggc
     3001 cgggattgcg cgcagcggag catacaaccg cacgctcacg ccgttcgggt tccagaacga
     3061 ggagcgcacg tactggcagg cgccggagat ctactacaag atgtcgccgt tttcctatgc
     3121 cgacaagatc aagacgccgg ttttgctcat tcatggcgag gcggacaaca actcggggac
     3181 attcccgatc cagagcgagc ggctgttcag cgccatcaag ggagccggcg ggacggtgcg
     3241 cttcgtgcta ttgccgctcg agtcgcatgg gtatgccggg cgggagtcgg tgctccacat
     3301 gttctgggag atgaacaact ggctcgatac gtatgtgaag aatccgcgag cggcgacggc
     3361 ggcgaagcaa gcggcgaatt gatggccaca agcattagga gctcgtccgg ggcgaaacga
     3421 gcctcaagga agctgcaggt cgaaggaata gatctcctgg gtgctgatgt cgcgggggaa
     3481 caggaaggtg tcgcccgggc ccaggccggt ccaggagccg gggccgaact ggctggggaa
     3541 acctctgatc ttcttcaagt cggccacctt ctccaatttg gcatcagtga tgcgcacgcg
     3601 atagtatccc gtgccgttcg tcagaatggt atcgaagtac acgtacgcgc tatccctgga
     3661 ccaggcaaga tagccaaacg aattcaggtt gatatcgagc tggcgccagg tctgcttctt
     3721 cacatcgaag agcatcatct cattcccatc gtgcgcaagg gcaacgatgt aacggccgtc
     3781 cggcgaccag cgaggaccaa agaaaccggt cgagcccgac agctgcgaaa tctggcgggt
     3841 cttggggtca aacagatcga tg
//
LOCUS       DASPUL010000407         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1296749, whole genome shotgun sequence.
ACCESSION   DASPUL010000407 DASPUL010000000
VERSION     DASPUL010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1296749"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            485..943
                     /locus_tag="VEJ00_09865"
     CDS             485..943
                     /locus_tag="VEJ00_09865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXX01508.1"
                     /translation="MLKLTKKADYALMAMKHLAEQPAPGSRSAKDVADAFGIPPEALA
                     KILQRLAKAGLLQSQHGINGGYTLARPAHTISAFEVIQAIDGPLFITSCVTVRGECGQ
                     SDRCNIREPLRKVNESIEAVLKRIKISHMREEPEEVASASGRPAELVTIT"
     gene            998..2290
                     /locus_tag="VEJ00_09870"
     CDS             998..2290
                     /locus_tag="VEJ00_09870"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HXX01509.1"
                     /translation="MSTDVKIPQDLNQNELPKTQHVAPKGVKLPIYMDNHATTPMDPR
                     VLEEMLPYFMEKFGNAASRNHSFGWAAEEGVETARERIAKLVGATTKEIIFTSGATES
                     DNLAIKGVAEMYREKGNHIITAVTEHKAVLDTCKRLEKYGYRVTYLPVQKDGLIDLDD
                     LKRAMDDKTILVTIMFANNEIGVLQPVEEIGKLCRERGVIFHSDATQAVGKIPVDVNK
                     QNIDVMSISGHKMYGPKGVGALYVRRKNPRVQVSAIIDGGGHERGMRSGTLNVPGIVG
                     LGKACAIAQEEMPKESCRMAGLRNRLRDRIMNRLDESYINGSMEHRLPGNLNISFAYV
                     EGESLLMGINDVAVSSGSACTSATLEPSYVLKALGTGDDLAHSSIRFGLGRFNTEAEV
                     DYVADRVIETVERLRELSPLYEMAKEGINLKDVKWAGE"
     gene            2377..>2573
                     /locus_tag="VEJ00_09875"
     CDS             2377..>2573
                     /locus_tag="VEJ00_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HXX01510.1"
                     /translation="MAYSDKVIDHYNHPRNVGSMDKASPDVGTGLVGAPECGDVMKLQ
                     IKVNRETNVIEDAKFKTFGCGS"
BASE COUNT          592 a          714 c          806 g          461 t
ORIGIN      
        1 cactctttgc actcgacgaa ttcggcgtcc tcgtcccggc tgacgatctg cacgcgcggg
       61 tgcttgcagg gtggcttggg gtcaacgctt tgacgtgccg cgggcgactt tgacgtcata
      121 aatccggaag cgccctcgac aagagctgag cagagggacc agttcgcggg aatttcccgg
      181 tattttagaa cggcccggcc gcttgtcaag cccccaggcc tcatggctgc tgtattccga
      241 acagttaggc gaggcggtag cctgacacga ctatgaggcg ctcgcaagcg ggcgagccag
      301 gcactcctcg catcgaaagc tcgttctaaa cacttcaatc ctctgtagtt accgctcaat
      361 ggagatctcg tacaactggc tgggcgccct tgccacgata agctgggttg tcgtaaaatg
      421 aagtacgacg aaaaaggtcg ggtttctatc cgaccctatt ggtcggattt acacaggccc
      481 gacagtgctc aagctcacga aaaaagcgga ttacgcgctg atggccatga agcatctggc
      541 cgagcagccc gcgccgggat cgcgtagcgc aaaggatgtt gccgatgctt tcggcattcc
      601 gcccgaagcg ctggccaaga ttctgcagag gctggccaaa gcgggcctgc tgcagtcgca
      661 gcacggcatc aacgggggct atacgctggc aaggccggcc catacgattt cggcttttga
      721 ggtgatccag gccatcgatg ggccgctgtt tatcacttcg tgcgtaactg tccggggcga
      781 atgcggccaa agcgaccgct gcaacattcg cgagccgttg cgcaaggtca acgaaagcat
      841 cgaggccgtg ctgaagcgca tcaagatttc gcacatgcga gaggagccgg aagaagttgc
      901 gagcgcaagt ggaagaccgg cggagctggt gacaatcacc tgattcgccg agcatagagg
      961 attcgtccaa agagatttta ggcaggagag gcaatcaatg agcacggatg tgaagatccc
     1021 ccaggatttg aaccaaaacg aacttcctaa gacgcagcac gtcgcgccca agggcgtgaa
     1081 gctgccgatt tacatggata accacgccac cacgcccatg gacccgcggg tgctggaaga
     1141 gatgctgcct tatttcatgg agaagttcgg caacgcggcc agccgcaacc actccttcgg
     1201 ctgggccgct gaagaaggcg tggaaacggc gcgggagcgc attgccaagc tggtcggagc
     1261 gaccacgaaa gagatcatct tcacttcggg cgccaccgag agcgacaacc tcgccatcaa
     1321 gggcgtggct gagatgtacc gcgagaaggg caaccacatc atcaccgcgg tcaccgaaca
     1381 caaagcggtg ctcgacacct gcaagcggct ggagaagtat ggctatcgcg tgacctacct
     1441 gccggtgcag aaagacggcc tgatcgatct cgacgacctg aaacgcgcca tggatgacaa
     1501 aaccattctg gtcacgatca tgtttgcgaa taacgagatc ggcgtgctgc agccagttga
     1561 ggagattggc aagctgtgcc gcgagcgcgg cgtaatcttc cacagcgacg cgacgcaagc
     1621 tgttggcaaa attccggtcg atgtgaacaa gcagaacatc gacgtgatgt cgatctccgg
     1681 gcacaagatg tacgggccca agggcgtcgg cgccttgtat gtccggcgca agaatccgcg
     1741 cgtgcaggtt tccgccatca tcgacggcgg ggggcacgag cgtggcatgc gctcgggcac
     1801 gttgaacgtc cccggcatcg tgggactggg gaaagcgtgc gccatcgcgc aagaagagat
     1861 gccgaaggaa tcctgccgca tggcgggcct gcgcaaccgt ctgcgcgacc gcatcatgaa
     1921 ccggctggac gagtcctata tcaacggctc aatggagcat cgccttcccg gcaatctgaa
     1981 catcagcttt gcctacgtcg agggcgagtc gctgctgatg ggcatcaacg atgtcgcggt
     2041 ttcgagcggt tcggcgtgca cctctgcgac gctggagcca tcttatgtat tgaaggctct
     2101 cggcactgga gacgacctgg cgcacagttc gattcgcttc gggctcggcc ggttcaacac
     2161 cgaagccgag gttgactatg tggccgatcg cgtcatcgaa acggtggaac gcctgcgcga
     2221 actgtctccg ttgtacgaga tggcgaagga aggcatcaac ctgaaggacg taaagtgggc
     2281 cggagagtag taccgagttg cgaggagtgg ggcaagtggg gaaaggctga gtcccgcagg
     2341 gacggcacta gatactagaa ggcttaggag aagcaaatgg catacagcga caaagtcatc
     2401 gatcattaca accatccgcg taacgtgggc tcgatggaca aggcgagtcc ggacgtgggc
     2461 accggcctgg tcggcgcgcc cgagtgcggc gacgtcatga agctgcagat caaggtcaat
     2521 cgcgaaacca acgtcattga agacgccaag ttcaagacct ttggatgcgg ctc
//
LOCUS       DASPUL010000408         4232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1849804, whole genome shotgun sequence.
ACCESSION   DASPUL010000408 DASPUL010000000
VERSION     DASPUL010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849804"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VEJ00_09880"
     CDS             complement(<1..501)
                     /locus_tag="VEJ00_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXX01511.1"
                     /translation="MSTQWTRPDGSSPTLYLRLQSNLEKAIREGELSPGSKLPAEREL
                     AEQLRVSRTTVTNAYRELESKGLVRGYVGRGTYVCALPEPSNVPFAWRGKMSAVSLRL
                     SAESCIVNLTSVDPNLISFGVGCPALECFPIEEYSRIQSSILRSRAPEVLGVGPVAGQ
                     PALREAI"
     gene            702..1388
                     /locus_tag="VEJ00_09885"
     CDS             702..1388
                     /locus_tag="VEJ00_09885"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HXX01512.1"
                     /translation="MLGIVGGLGPESTIEYYRLLVSGYRERGASGSPPLFIHSIDVDT
                     VLRLAGAGKSEELAKYLLEAVLPLARAGAEFALIAANTPHLVFDAVARQSPVPLLSIV
                     EATCAFAKRNAHKKLTIFGTRSTMRAGFYQKALEREGIQVQLPSSEEQDYIHEKYVGE
                     LVKGRFLHETRAGLIAIARRLKQEEDIDGVILGGTELPLLLTDNGGLGIPFLDTTRIH
                     VDAALDRMLA"
     gene            1457..1990
                     /locus_tag="VEJ00_09890"
     CDS             1457..1990
                     /locus_tag="VEJ00_09890"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX01513.1"
                     /translation="MKKVIALLILSLAISGYAQDQKEAPTLRSILLEELKSTHNEKDW
                     FVDADTAVAGLTAEQANWTDGKGNHSVGQLAYHIVYWNQRNLARFKGETPEKFSGNND
                     ETFTQFDSKKWADTVRQLDAVMTEWEKAVETMDDKKLEEWAPVLSRIATHNAYHIGQI
                     VFVRKEQGSWDASKGVK"
     gene            2083..2526
                     /locus_tag="VEJ00_09895"
     CDS             2083..2526
                     /locus_tag="VEJ00_09895"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX01514.1"
                     /translation="MKQTDYSISTDTSRFDIDLIFRFLTTCYWAEGIPRDVVEKSIRN
                     SLCFGVFDGDKQVGFARVITDRATYAYIGDVFILESHRRRGLGKQLIQAIMEHPELQG
                     LRRWSLVTRDAHALYEQFGFTALAHPQRYMERHDPNVYTRRPGAK"
     gene            2823..3449
                     /locus_tag="VEJ00_09900"
     CDS             2823..3449
                     /locus_tag="VEJ00_09900"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HXX01515.1"
                     /translation="MRIAVIGTGHVGGALGAGWARAGHEVIFGTRELDAPELKSLLQS
                     APKASADSVLSSAARAEVTALAVPWSAVSAVLAEIGEQVRGKILIDCTNPATAWPALD
                     HSAGSGAEQIARLVPDARTVKAFNTTGFENMQNPKYGEGAVTMFYAGDDLGAKKVVHG
                     LAKDLGFDPVDAGGLTQAHALEILASLWGTLAYGQKMGRGIAFRLMRR"
     gene            3584..3757
                     /locus_tag="VEJ00_09905"
     CDS             3584..3757
                     /locus_tag="VEJ00_09905"
                     /inference="COORDINATES: protein motif:HMM:NF042615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6800 family protein"
                     /protein_id="HXX01516.1"
                     /translation="MSAPSRRPEIRRRRTRKEKITQLRKRLAAAKTDGDRARITAKLH
                     KLSIASPGQPLLK"
     gene            complement(3767..>4232)
                     /locus_tag="VEJ00_09910"
     CDS             complement(3767..>4232)
                     /locus_tag="VEJ00_09910"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXX01517.1"
                     /translation="IIGDDLSGNPDLRRTVVRSGVQNDVRFLGFVPIEVLRIFYDEAK
                     VFVFPSLYEGFGLPPLEAMAHGTPVVTSNVSSLPEVVGSAAVLVNPENVFEIMRALHR
                     VLTDRALRDRMKGRGYEQATRFSWEASVRRIMAVYGEVVGGRSARAGQPAAD"
BASE COUNT          914 a         1168 c         1354 g          796 t
ORIGIN      
        1 gatcgcctcg cgaagagcgg gctggccggc gacgggtcca actccgagca cctcgggcgc
       61 acggctgcgc aggatcgagc tctggatgcg gctgtactct tcgatgggga aacactccag
      121 agccgggcat ccgacgccga aggagatcag gttcgggtcc accgaagtga ggttgacgat
      181 gcagctttcc gcgctgagcc gcaacgacac ggcggacatt tttccgcgcc aggcgaaagg
      241 aacgttgctt ggttccggca acgcgcagac gtatgtgccg cggccgacat atccgcgcac
      301 caggcctttg gattcgagct cacgataggc attggtaacg gtcgtccggc tcacgcggag
      361 ctgctctgcc aactcgcgtt cggcgggaag tttcgagccc ggggatagtt caccctcccg
      421 gatcgccttc tccaggttgg attgcaggcg caggtaaagg gtgggggagg aaccgtcggg
      481 gcgtgtccac tgagtgctca tcgcattctc gactccgttg tccaggttga aacgggggaa
      541 aactacggca cttgaagaga tagagtcaga caaagctccc cggattcaca aaatacgacg
      601 cctgtgatgg actgccaagc agccaattgt ggaatggata tttggcccgc cggaggctag
      661 tctacgatgc gaaaatgggg tgaaatttgg gcaaaggcag aatgctgggt atagtcggcg
      721 ggctggggcc ggaatcgact atcgagtact atcgcctgct ggtttccggg tatcgcgaac
      781 ggggcgcgag cggttccccg ccgctgttta ttcacagcat cgatgtcgat acggtgctac
      841 ggcttgctgg ggcggggaag agcgaagagt tggcgaaata tctgctcgag gctgtattgc
      901 cgctggcgcg cgccggcgca gagtttgcct tgattgcggc caacacgcct catcttgtct
      961 ttgacgccgt tgcgcggcag tcgccggttc cgttgctcag catcgtggaa gcgacctgcg
     1021 cctttgccaa gcggaacgcg cacaaaaagc tgacgatttt cggtacacgc tcgaccatgc
     1081 gggcggggtt ttatcagaag gctcttgagc gcgagggaat tcaggtacag ctacccagct
     1141 ccgaggagca ggactacatt catgagaagt atgttggcga actggtgaag ggacgctttc
     1201 tgcacgaaac gcgcgcgggt ctgattgcga tcgcccggcg cctgaaacaa gaggaagaca
     1261 ttgacggcgt gatccttgga ggcaccgaat tgcctctgct tctgaccgac aatggcgggc
     1321 ttggcattcc tttcctcgac acgacccgga ttcatgtgga tgccgcgctc gaccggatgc
     1381 tggcgtgaac gctgccgagt cttgaacgct cgccaattga ggcttcggcg gtctaagaga
     1441 caaggaggtg atctgcatga aaaaagtgat cgcgctactc atactttcgc tggcaatttc
     1501 gggctatgcg caagatcaga aagaggcgcc gacgctgagg agcattctgc tcgaagagct
     1561 gaaatcgacg cacaatgaga aagactggtt tgtcgatgcc gacactgccg tggctggact
     1621 gacggcggaa caggctaact ggacggacgg gaaaggcaat cactcggtag ggcagctcgc
     1681 ctatcacatt gtttactgga accagcgcaa cctcgccagg ttcaagggtg aaactccgga
     1741 gaaattcagc ggcaacaacg acgaaacctt cactcagttc gacagtaaga aatgggcgga
     1801 caccgtacgg caactcgacg ccgtcatgac cgagtgggag aaggcagtcg agaccatgga
     1861 cgacaagaaa ctggaggagt gggctccggt cctctcacga atcgcgacgc acaatgccta
     1921 tcacatcggg cagattgtat ttgttcggaa ggagcaggga tcgtgggatg catcgaaggg
     1981 agtcaagtag gggggcgaca tacgttttgg aaggggtgca ttctgtcccg gtagcgcctg
     2041 ctggaatacg atgaaccggc gggcgcgact ggagccctgg cgatgaagca gaccgactat
     2101 tcgatttcta ctgacacctc gcgattcgat atcgacctga tattcaggtt tcttactacc
     2161 tgctactggg cggaaggaat tccacgcgat gtcgtggaga agtccatcag gaattcgttg
     2221 tgcttcggcg tgttcgacgg cgacaagcag gtcgggttcg cccgcgtgat cactgaccga
     2281 gcgacatacg cctatatcgg tgatgttttc atcctcgagt cgcaccgcag gcgcggactg
     2341 ggcaagcagc tgattcaggc aatcatggag catcccgagc tgcaaggttt gcggcgctgg
     2401 tcgctggtca cgcgcgatgc gcacgcgttg tatgagcaat tcgggttcac tgctctcgcc
     2461 catccgcagc gatacatgga gcggcacgac ccgaatgtct acacccgtcg gcccggggcg
     2521 aagtaggtct acacggccat ccgcctgccg gcatgtgcgc gtctgccaac acaccttcag
     2581 gtaagtcact attctctagg tgcctagcac gtgcaccctt cgacgtgcta aatagtctca
     2641 acgcgatatt cagtgtcccc tgcctaaacc tgccagatca caagcggcgg gaattctttg
     2701 cgttgcggag cctcgctcgc gctgagaaag gagaaaggtg tgagtgtcag caacaggcgt
     2761 gcccgccgga tgcgacggac tgatggcaga gcggcaacaa tttctgaaag cgaggtcaca
     2821 agatgcgaat tgcagtcatt ggaaccggtc atgtaggagg cgctttgggc gcgggctggg
     2881 cgcgagcggg gcacgaagtg attttcggaa cccgcgagct cgatgctccg gaattgaaat
     2941 ccttgctgca gagtgcccct aaggcgagtg ccgactccgt gctcagctca gccgcacggg
     3001 ccgaggtgac tgctcttgct gttccctgga gcgcagtatc tgcagtgctt gcggagattg
     3061 gtgaacaggt tcggggcaag attctgattg attgcacgaa cccggccacg gcgtggcctg
     3121 cgctggacca ttccgcagga tcaggggcgg agcagattgc tcggcttgtg cctgacgcca
     3181 gaactgtgaa ggcgtttaac acaaccggct tcgagaacat gcagaaccca aaatacggcg
     3241 agggtgccgt cacgatgttt tacgcgggag atgacctggg agcgaaaaag gtagtacacg
     3301 gactggcaaa agacctgggt ttcgatcccg tagacgcggg cgggttgacc caggcgcacg
     3361 cactcgaaat tctggcgtcg ctgtggggaa cgctggccta cgggcagaag atgggacggg
     3421 gcatcgcctt ccggctgatg cggcgataac cgaaggaagc aaaggaagca agacacggcg
     3481 agttcgcgga ccgctgtcgc tggtgaccgc gaaaggcggc aagctggcat caggtaccgg
     3541 cagtccgcta tactcggcgc ttcacatttc cgaggaggat ggaatgtctg cacccagccg
     3601 acggccggag attcgccggc gccgtacccg caaagagaag attacccaac tgcgcaagcg
     3661 actggccgcc gccaaaaccg acggtgatcg cgcccgcatc accgccaagc tgcacaagct
     3721 ttcgatcgcg tcccctggtc agccgttgct gaagtaaggg ctggatctag tccgcggcgg
     3781 gttgcccggc gcgggcggac ctgccgccaa cgacctcgcc gtagacagcc atgatgcgcc
     3841 gcaccgaagc ttcccaggag aacctcgtgg cctgctcgta accacggcct ttcatgcggt
     3901 cgcggagggc gcggtctgtc agaacgcggt gcagggcgcg catgatctcg aagacgtttt
     3961 cgggattgac cagcaccgcg gcgctgccca ccacctcggg cagcgacgac acgttggaag
     4021 tgaccactgg cgtcccgtgc gccatggctt ccagcggagg caagccgaaa ccttcatata
     4081 acgacggaaa gacgaagacc ttggcctcgt cataaaaaat ccgcagcact tcgatcggga
     4141 cgaatccgag gaagcggaca tcgttctgca cgccgctgcg gacgaccgtg cggcgcagat
     4201 caggattgcc ggagaggtca tcaccaatga tg
//
LOCUS       DASPUL010000409        10670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1441667, whole genome shotgun sequence.
ACCESSION   DASPUL010000409 DASPUL010000000
VERSION     DASPUL010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10670)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10670
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441667"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VEJ00_09915"
     CDS             <1..744
                     /locus_tag="VEJ00_09915"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXX01518.1"
                     /translation="EGGPRVLPAYPEDLSHSAEEQLRRLGVEVRTSTMVNQIEPGAVH
                     FGGTRLAASVVLWAAGVAASPLGRKLGMPVDRAGRVLVQPDLSLPGHPEVFVIGDLAA
                     AKDEQGKMLPGVAPVAIQQGRYVAKIIRREITQAKAGAGSRPAFHYWNKGSLATIGRA
                     AAVADFGKIHISGFMAWLSWLFVHIWFLIGFRNRLLVFIQWAWSYFTYERGARLITGS
                     TYLPGWASSAAPATVAATAPQEIHTSAGK"
     gene            804..2138
                     /locus_tag="VEJ00_09920"
     CDS             804..2138
                     /locus_tag="VEJ00_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HXX01519.1"
                     /translation="MKIFLPFRLLFSAAILIFCWRPAAADVLKITIDDTIQPITAEYI
                     SRAIDEAQHRNAQAVLIELNTPGGLVTSTREIIERITSSPVPVIVYVTPTGGHAGSAG
                     IFILESADIAAMAPGTAAGAAHPVALIGPVQLKPDEEMNRKIENDAAALMRSVAGKRG
                     RNTEAAESAVRESKSFTDQEALAQHLIEYVASSPDDLFRQMQGKSFKRFNGETTSVNL
                     VGQAMVPFYMTVKEHVLDVLMNPNIAFLILIIGALSLYIEFNHPGAVVPGTVGVVFIL
                     LALFALNLLPTRFAALVLILGAFALFAAEAKFATHGVLTVGGIALITIGGLLLVDSPI
                     PEMRVHLLTALAVSIPLGVITAFLMTIAVRARRNKIVTGAQGLVGETGVVQTALSPQG
                     KVFVHGELWDAVASTSLPAGQMVVVRKVDGLILEVAPLAAMPSSGKRAAAVL"
     gene            complement(2313..3038)
                     /locus_tag="VEJ00_09925"
     CDS             complement(2313..3038)
                     /locus_tag="VEJ00_09925"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HXX01520.1"
                     /translation="MKSFCVFFASLVFAVSAFTQDHPALTALPNTVYVGADGKCESAP
                     DTAQVQFHISVQEATPQAAFQKASKNVDDVRQVLRANGIEPAAANIGFLSVQPTYQWD
                     KGKQTLIGYRVTSDVTLKLKDFTKVGPITQQLADANISETQTLNYTLQNMDDAKNKAV
                     EDAYRRARNSAETLAHASGRTLGELLYASIDTFEAPRLFTPRPMMASMKAMAAATPPA
                     PTEEFTPQTVTVTAHVNALFNLK"
     gene            complement(3280..3927)
                     /locus_tag="VEJ00_09930"
     CDS             complement(3280..3927)
                     /locus_tag="VEJ00_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01521.1"
                     /translation="MPCRILRLRVFVVTASVLALSAALLHAQDTPSAPANAVMQGTVC
                     LIQLADRLDTRMVKAGDHFHARLAEPLMTADGTTIEAGRKVTGHVSAVEPGLTRRLLL
                     SFDEIETPRGWMPLIATVTGVPGEHGLKQIGDEGEIGRKAMTKEQIAEAVVVGAGEGA
                     AEGARNGGKRGAATGAGSGAADSALSAFESSHDLALEKGTALEIRLDRNLMIPAR"
     gene            complement(4020..5654)
                     /locus_tag="VEJ00_09935"
     CDS             complement(4020..5654)
                     /locus_tag="VEJ00_09935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXX01522.1"
                     /translation="MRHPKLWVGAAAFLTAALALACAFLPQGIARVAVSDIVSAALML
                     SALLAFAADGRASKGRLRWFWMLQAAGWTLWLGDQIVWIVYDLILKHKMPPMSPADAL
                     LFLAGAPMLAGLLLRPDRQPSDRSARLGVLDFLILFLWWLYLYLSFVVCWQYVSPNQG
                     AYDRSFDVLTGVEYFLLSGVVVAFWRQSSDRWKTFYACFFGAVVFNGIAYYVLNLAIE
                     KGTYFTGSWYDIPYAASFAMFTAVAMLGRGLVPARETPADDSYDSWMANLAMTAVLSL
                     PVIALFALLDSRLAPEAVHFRVLITLVTVFVMALLLFIQHHRLNRELRSTNVVLQEAS
                     LTDPLTSLRNRRYFSATIESDVSRTLRSYADGHDPHTRDLVFYLIDVDNFKEINDRYG
                     HDVGDRVLIEMTRRLSSSIRHSDVLMRWGGEEFLIVSRYTDRREAELLAQRVLAAVAD
                     APFALSRPEETIYRTCSMGWAAYPWFPHDPHAISYDEVLTLADRGLNQAKKAGKNRAV
                     GMLPASGEVISAATEQMHSMEIQVAALSVAGPRSAV"
     gene            complement(5739..6827)
                     /locus_tag="VEJ00_09940"
     CDS             complement(5739..6827)
                     /locus_tag="VEJ00_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXX01523.1"
                     /translation="MPDRILVVDDEEPIREIVASMLGAAGYACKHAGSGKEALAVLTS
                     GEEFELMLSDLMMADLDGIGLLERTKEKYPDMPVVMVTAVHDISIALQAIRNGAYDYL
                     LKPFEREQLLNTVSRALENRRLKVENRTYQTNLESLVRARTDQLQATLRDLERSYDIT
                     LQALGDALDLKDRETEGHSRRVTAFTMAIARAMGLPAETVATIARGAFLHDIGKMAIP
                     DKILNKPGKLDPEETAIMREHCFKGYQIVKKIPFLTDACDIIYSHQERFDGTGYPRGL
                     KGKEIPLGARIFSVADTLDAITSDRPYRAARPLAAARKEIQDWSGRQFDPEVVTVFQQ
                     MPDEIFEELRRAISAQTDPLAYYASAKD"
     gene            6965..10477
                     /locus_tag="VEJ00_09945"
     CDS             6965..10477
                     /locus_tag="VEJ00_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXX01524.1"
                     /translation="MSAPLSSLWNTAVDLGITSLTAGEVALLILLGASLLSLRALREN
                     YFKVWIVGWAVLVASRLMEHPFAARFPAPFGEVAVQASFVLAAGLLTGAVLVFTRSKN
                     LIVPLAVITPLLVGFAGARVLLAPDSLPLRMALEISYRTLLLTASVALLRARRGRWEP
                     ASWLLALCLPCLHLAWAPLTARVPDAAFLAAEITLGASMLLVVYGDARMRARRLQAMQ
                     TLSTSIAGAHQYGNMVQSALEELQRLTGVRAAWFRLLEGENLVATHAVGLSADFMRDS
                     GFAPVTEDVTNLLQNPEPLVKTRNAAAPEAEGSLENEKIRQLVLAPVVGNKSPIGLLF
                     LGHSRKRHWTRDELQFLQTCAQQLAVAVENSRLLEKVLRSQSQWMNTFDSIHDIILAH
                     DADYRIIKVNQVLLGHLGQSAADVIGSTCESALPHTYGEWTGCPYCAMRGDEEFTEGA
                     DPCFGGFSVVSTSSYTEQESSQKGIIHVVRDTTERRSAEERYRLLFEQVQEGVYIATT
                     EGRLLDCNDALLHMLGYSSREELLVLNLNSEICVDARQRENFHKEMEAHNYVRNFEVT
                     MRRKDGTVLLAVESCFATRDATGNIERYQGFVLDMTEKRRAEDEMSRRNRELNALNAM
                     AVVAAQSFDLDEILNLTLRQVVTLFGAESGSVYLSDADGTMFRRRAAWGPRKTGKAQP
                     AEIAFTDGFGDLVMRSRTEVVTAEYLPHLPKNVTEFLRSDADRSWIWVLFWGKDAPVG
                     IMGLCNQVGHEYSSNDENLLVAISRQLATTIEKVRLYEETCRAYEDLRKTQEQLLQSE
                     KMSAVGQLIAGVAHELNNPLTAILGYAQLLESEGLNTRAQDYITKLFKQAQRTHRVVQ
                     NLLSFARQRQPQREEVDLRKVLDETLALRDYDLKTNNIQVEREAQADPAMVVADPHQI
                     EQVFLNIINNAVDAILETGGTGKLRIRVYTENGHVATQFADDGAGIKDPKRIFDPFYT
                     TKSVGKGTGLGLSICYGIVKEHGGDITARNAAEGGAVIEVRLPTAAPVAAQPDAAKPA
                     PRPYEAAVHGRILLVEEEEAVLEFERDVLTGAGATVVTARTSHDVKSKLTSDSIEAVI
                     MAGKMPGDWNAKESYTWIKENCPAMVGHVLFTFSNGVEQGDGRAFLQENGIPYLVKPF
                     EVAELIAQTRKLLQKAQAAGAGAS"
BASE COUNT         2052 a         3134 c         3514 g         1970 t
ORIGIN      
        1 gaaggtgggc cgcgggtgct tcccgcttat ccggaggatc tgtcccacag cgctgaggag
       61 cagctgcggc gtctcggtgt cgaggtacgc acatcgacga tggtgaacca gatcgagccc
      121 ggagccgtgc acttcggcgg cacgcgactg gctgcctcgg tcgttctttg ggccgcggga
      181 gtagcagcgt cgccgctggg cagaaagctg ggaatgcccg tcgaccgcgc aggacgtgtg
      241 ctggtgcagc ccgacctgag ccttcccgga catccggaag tgttcgtcat cggcgatctg
      301 gccgcggcca aggatgagca gggcaagatg ctgccgggcg tcgcgccggt tgcgattcag
      361 caggggcgct acgtggcgaa gatcattcgg cgagaaatca ctcaagcgaa ggcgggcgct
      421 ggctcgcgtc ctgctttcca ctactggaac aaaggcagtc tggccacgat cggccgcgcg
      481 gccgccgtgg cggatttcgg caagattcac atttccggct tcatggcgtg gctgtcatgg
      541 ctgttcgtcc atatctggtt tctgatcggg ttccgcaacc ggctgctggt tttcatccag
      601 tgggcatggt cctatttcac ttacgaacgc ggtgcgcggt taatcacagg cagtacctac
      661 cttccgggat gggcgtcgag cgccgcgccg gcaaccgtgg cagcgactgc gccacaagaa
      721 atccacacca gcgccggcaa atagagggaa aatagaaatg ggctggcgtc tccggtagcc
      781 cacccgctac gagtgtgaaa gaaatgaaga ttttcttgcc ttttcgcctg cttttctcag
      841 ccgcgatttt gattttctgc tggcggccgg ctgcggccga cgtgctcaag atcacgattg
      901 acgacacgat tcagcccatt accgccgagt acatttcgcg cgccatcgac gaagcgcagc
      961 accgcaacgc gcaggcggtg ctcatcgaat tgaacactcc cggcgggctg gtgacctcga
     1021 cccgcgagat catcgaaagg atcacaagct cacccgtgcc ggtgatcgtc tacgtcacgc
     1081 ccaccggcgg ccacgcgggc tcggccggga tatttattct cgaatcggcc gacattgcag
     1141 ccatggcgcc cggcacggcc gcgggcgcgg cgcatccggt ggcgctcatc ggaccagtgc
     1201 agctcaagcc cgatgaggag atgaaccgca aaatcgaaaa cgacgcggcg gcgctcatgc
     1261 gctcggtcgc gggcaagcgc gggcgcaaca cggaagcggc ggagagcgcg gtgcgcgaat
     1321 cgaagtcttt tacggaccag gaggcgctgg cacagcatct catcgagtac gtcgcttcca
     1381 gcccggatga cctgttccgc cagatgcagg gcaagagctt caaacgcttc aacggggaga
     1441 ccacttcggt caatcttgta ggccaggcaa tggtgccgtt ttacatgacc gtcaaggagc
     1501 acgtgcttga cgtgctcatg aaccccaaca tcgcgttcct catcctcatc atcggcgcac
     1561 tttcgctgta catcgagttc aatcatccgg gcgcggtggt tccgggaacg gtgggagtcg
     1621 tattcattct gctggcgctg ttcgcgctca atcttctgcc cacgcgtttc gccgcgctgg
     1681 tgctgatcct tggggcgttt gccctgtttg ccgccgaagc aaagtttgcc acgcatggag
     1741 tgctcacagt cggcggaatc gcgctgatca cgatcggcgg actgctgctg gtcgattcgc
     1801 ccattccgga aatgcgcgtg cacctgttga cggccctcgc ggtgagcatt ccgctgggcg
     1861 tgattactgc gtttctgatg acgatcgccg tccgggcgcg caggaacaag atcgtgaccg
     1921 gggcgcaggg cctggtcggg gagactggcg tcgtgcaaac ggcactctct ccgcaaggga
     1981 aagtctttgt gcacggggaa ctgtgggatg cggtggcctc cacttccctt cctgcgggac
     2041 agatggtggt ggtgcggaag gtcgacgggc tgatccttga agtggcgccg ttggcggcta
     2101 tgccgtcctc gggcaagcgg gccgcggcgg tgttgtagga gatcgtcgaa gactgcgttc
     2161 gcgtgggaac gcgccaccac caaggctaaa gcccgtcggg ttttcaacgc ctgacgcggc
     2221 tctaaagggc cgctcctcca cggggatccc aacgccgcgc acggcgatcc caacaccccc
     2281 ggccttcagg ctccggcaat gcgatccggc ctctacttca gattgaacag cgcgttcacg
     2341 tgggccgtta cggtcaccgt ctggggcgtg aattcttcgg tcggggcggg cggagtggct
     2401 gcagccattg ccttcatgga agccatcatc gggcgcgggg tgaacagacg cggggcctcg
     2461 aaggtgtcaa tcgaggcgta gagaagctct ccgagcgtgc ggccgctggc gtgggccaac
     2521 gtctcggccg agttgcgcgc gcggcggtag gcatcttcca cggccttgtt cttggcgtcg
     2581 tccatgttct gcaaggtgta gttgagcgtt tgcgtctcgc tgatgttggc gtcggcgagc
     2641 tgctgcgtga tcgggcccac cttcgtgaaa tccttgagct tcagcgtgac atcgctggtg
     2701 acgcggtatc cgatgagtgt ctgcttgccc ttgtcccact gataggtcgg ttgcacggag
     2761 aggaatccga tattggcggc cgccggctcg atgccgttgg cgcgcagcac ctgccgcacg
     2821 tcgtccacat tcttcgaggc cttctgaaag gccgcttggg gcgtcgcttc ctgaacggag
     2881 atgtggaact gaacctgcgc cgtgtcgggc gcggattcac atttgccgtc ggcgcctacg
     2941 tagacggtgt tcggcagggc ggtgagggcc gggtggtcct gcgtaaatgc cgaaacagcg
     3001 aagaccaagc tggcaaagaa aacgcagaag gatttcatgg atggacctcc gaggcgatta
     3061 tcaaacagcg gagcgcgttt gccaaaatgc tccccctcgg aagaacgttg cttgggatgg
     3121 atcttgccgg tgtgaaatag tgccgccgcc tcggcggcaa tcacgggagc gtcgagtccc
     3181 gcagccgagg gcggaacgcc ctcgcgactt cggcaggatg ccagccctat gaccggtggg
     3241 cgccgaggtt tctggcagtc cgtgatacta gttccaggct caacgcgcgg gaatcatgag
     3301 gttgcgatca aggcggatct cgagagccgt gcctttctcg agtgccagat cgtgactgga
     3361 ttcgaacgcc gacaatgcgc tatcggccgc gccggatccg gcgccagtgg cagcgccgcg
     3421 cttgccgccg ttgcgcgcgc cctcggccgc gccttcgccc gctccaacga cgacggcttc
     3481 ggcgatttgt tccttggtca tggctttgcg gccgatctcg ccttcatctc cgatttgctt
     3541 caggccgtgt tcgccgggaa cgccggtgac ggtagcgatg aggggcatcc atccgcgagg
     3601 cgtttcgatc tcatcgaagc tcaggagcag gcgtcgggtg agtcccggct cgacggcgct
     3661 gacgtggccg gtgactttcc tgccggcctc gatggtcgtg ccgtcagcgg tcatgagcgg
     3721 ctcggccagc cgggcgtgga agtgatcgcc agccttcacc atgcgcgtgt cgagacgatc
     3781 ggcgagctgg atgagacaca cagtgccctg catgaccgca ttggcaggag ccgacggtgt
     3841 atcttgcgcg tggagcagag cggcggaaag agcgaggacc gaagcggtaa cgacgaacac
     3901 acgcagcctg aggatgcgac agggcatgga agtctcccgg aaagatggat tcttgcaaag
     3961 ttggatgtga ggacgccgag aattgctgcc tgctggctag gacaaaatgg caggcttggc
     4021 tagacggcgg accgcggtcc ggccacagac agggcggcta cctgaatttc catggagtgc
     4081 atctgctcgg tggcggcgga aatcacttcg ccgctggccg gcaacatacc gacggcgcgg
     4141 ttcttgcctg ccttcttggc ctgattgagg ccgcgatcgg cgagggtgag cacctcgtcg
     4201 taactgatgg catggggatc gtgggggaac cacgggtaag cagcccagcc catggagcag
     4261 gtgcgataga tcgtctcctc aggccgggac agggcgaagg gcgcgtcggc cacggccgca
     4321 agcacgcgct gggccagcag ctcggcttcg cggcggtcag tgtagcggga gacgatcaga
     4381 aattcttccc cgccccagcg catcagcaca tcggaatggc ggatggagga actcagccgg
     4441 cgggtcatct caatcagcac gcggtcgccg acgtcgtgac cgtagcggtc gttgatttcc
     4501 ttgaaattgt cgacatcgat caaatagaaa accagatcgc gggtgtgggg atcgtgaccg
     4561 tcagcgtagg agcgcagcgt gcggctgacg tcgctttcga tggtggccga gaaataacgt
     4621 cggttgcgca agctggtcag ggggtcggtg agcgaagcct cctgcagaac cacgttggtg
     4681 ctgcgcagct cgcgattcag gcggtgatgc tggatgaaca gaagcagcgc catgacaaag
     4741 acggtcacca gcgtgatcag cacgcggaaa tgtacagcct cgggggcgag ccgcgaatcg
     4801 agcaaggcaa agagggcgat gaccggcagg gacagcacgg ccgtcatggc caggttcgcc
     4861 atccaggaat cgtaagagtc gtctgcggga gtttcgcgcg ccggcaccag gccgcgtccc
     4921 agcatagcca cggcggtgaa catggcgaac gaggccgcat aaggaatgtc gtaccagctg
     4981 ccggtgaaat aggttccttt ttcgatagcc agattaagga cgtaatatgc aatgccgtta
     5041 aagacgactg cgccgaagaa gcaggcgtag aaggtcttcc agcgatcgga actctgccgc
     5101 cagaaggcga cgacaacgcc gctcagcaag aaatactcca cgccggtgag cacgtcgaag
     5161 ctgcggtcgt acgccccttg attgggcgac acgtactgcc agcagaccac aaacgacagg
     5221 taaagataga gccaccacag gaacaagatc aggaagtcga ggactcccag acgagcgctg
     5281 cggtcgctcg gctggcggtc gggacggagc agcagccctg cgagcatggg cgccccagcc
     5341 agaaacagca aagcgtccgc aggagacatc ggcggcatct tgtgcttgag gatgagatcg
     5401 tagacgatcc agacgatctg atcgcccagc cagagcgtcc agcccgccgc ctgcagcatc
     5461 cagaaccagc gcaggcggcc cttgctagcc ctgccgtcag cggcaaaggc gagcagcgca
     5521 ctcagcatga gggcggcgct gacgatgtcg ctgaccgcga cccgagcgat cccctgcggc
     5581 aaaaaggcgc aggccagcgc cagcgccgcg gtcaggaaag cggcagcgcc cacccacagt
     5641 ttcggatgac gcataggatt caagtcgtcg tccccggtca atcctttggc ccacacagtc
     5701 ttgctcgttg cttaccacta ccctgggatg ggttgtgctt aatccttggc gctggcgtaa
     5761 taggccagtg ggtcggtctg cgcgctgatg gcgcggcgca gctcttcgaa gatttcgtcc
     5821 ggcatctgct gaaagactgt cacgacttca gggtcaaact ggcggccaga ccagtcctga
     5881 atctctttgc gtgcggcagc gagggggcga gctgcccggt acggccggtc cgaggtgatg
     5941 gcatcgagcg tgtcggccac ggagaagatc cgcgccccga gagggatttc tttccctttc
     6001 aagccgcgcg gatagcctgt gccgtcgaag cgctcctggt gggagtagat gatgtcgcag
     6061 gcgtcagtga gaaacggaat cttcttgacg atctggtagc ccttgaagca gtgctcgcgc
     6121 atgatggcgg tctcttcagg atcgagcttg ccgggcttat tcaggatctt gtcggggatt
     6181 gccatcttgc cgatgtcatg cagaaacgca ccgcgcgcga tggtagccac ggtttcggcc
     6241 ggcaatccca tggcgcgggc gatggccata gtaaaagcgg tgacgcggcg ggagtggcct
     6301 tcggtttcgc gatccttaag gtcgagagcg tcaccgaggg cctgcagcgt gatgtcgtag
     6361 gagcgctcca ggtcgcgcag cgtggcctgc aactggtcgg tacgggcgcg gacgagcgat
     6421 tccagattgg tctggtaggt gcggttctcg actttgagcc ggcggttctc gagcgcgcgg
     6481 ctgaccgtgt tgagcagttg ctcgcgctcg aacggcttca gcagatagtc gtaagcgccg
     6541 ttgcgaatgg cttgcagggc gatggagatg tcgtggactg cggtcaccat caccaccggc
     6601 atgtcgggat acttttcctt cgtgcgttcc agcagaccaa tgccgtcgag gtcggccatc
     6661 atgagatcgg agagcatcag ttcgaactct tcgccggaag tgagcacggc gagcgcctcc
     6721 ttgccggaac ccgcgtgttt acaggcatat ccggcggccc ccagcatgga ggcgacgatt
     6781 tcccgaatcg gctcttcgtc gtcaacgaca agaatgcggt ctggcatgga aatacacgaa
     6841 agtctatcgg cgattgtaac ccagtagcga gatgccgaaa gtaatcaacg aggtccggaa
     6901 actccatcga aaaacagtca gcgcgtgata ttcagaagcg aggaattcgt acgttcgtaa
     6961 cccgatgagc gcaccccttt cgtcactttg gaacacggcc gttgatctgg gcattaccag
     7021 cctcacggcg ggcgaagtgg ccctgctgat cctgttgggc gcctcgctgc tcagcctgcg
     7081 cgcgctgcgc gaaaactact tcaaggtctg gattgtcggc tgggcggtgc tggtcgcctc
     7141 gcggctgatg gagcatccgt ttgcggccag gtttccggcg ccgtttggcg aagtggcggt
     7201 gcaggcgagt tttgtgctgg cggcgggact gttgacggga gccgttctgg tcttcacgcg
     7261 cagcaagaat ctgatcgttc cccttgccgt gatcacgccg cttttagtgg gttttgccgg
     7321 agcgcgcgta ctcctggcgc ccgattccct gcccttgcgg atggcgctcg agatcagcta
     7381 ccggactctg ttgctgaccg cctcggtggc gcttctgagg gcgcgacgcg gtcggtggga
     7441 gccggcttca tggttgctgg cgctgtgtct gccctgcttg catctggcgt gggcgccgtt
     7501 gacggctcgt gtgcccgacg cggctttcct ggcggctgaa attaccctcg gcgcgagcat
     7561 gttgctggtg gtttatggcg acgcacgcat gcgggcgcgc aggctgcagg cgatgcagac
     7621 gctgagcacc agcattgcag gcgcgcacca gtacggcaat atggtgcaat cggcgctgga
     7681 agaactgcag cgccttactg gcgtacgtgc ggcgtggttc cggctgttgg agggcgagaa
     7741 tctggtcgcc acgcacgccg tcggactctc cgccgacttt atgcgggact cggggtttgc
     7801 cccggtcacc gaggacgtaa cgaacctgct gcagaatccc gagccgctgg ttaagacgag
     7861 gaatgcagct gcccctgagg ccgagggatc gcttgagaac gaaaaaatcc ggcaactggt
     7921 tctggctccg gtagtgggca acaagtcgcc catcggcctg cttttcctgg ggcattcgcg
     7981 gaagcggcac tggacgcggg acgaactgca gttcctgcaa acctgtgccc agcagctggc
     8041 cgtcgcggtg gagaactcgc ggctgctcga gaaagtgctg cgctcgcaga gccagtggat
     8101 gaataccttc gattccatcc acgacatcat cctggcgcac gacgcggact atcgcatcat
     8161 caaggtcaat caggttctgc tcgggcatct gggccagtcc gcagccgacg tgatcggcag
     8221 cacctgtgag tcggccctgc cccataccta cggcgaatgg accggttgcc cgtactgcgc
     8281 catgcgtggg gacgaagagt tcaccgaggg agccgatccg tgcttcggcg gcttctcggt
     8341 ggtttcgacg tcgtcctaca ccgagcagga gagctcgcag aaaggaatca tccacgtcgt
     8401 gcgcgacacc accgagcggc gctcggcgga agaaagatat cgattgctct tcgagcaggt
     8461 gcaggaaggc gtgtatatcg ccacgaccga gggacgcctg ctcgactgca acgacgcgtt
     8521 gctgcacatg ctcggctaca gcagccggga agagctgctg gtgctgaacc tgaattcgga
     8581 gatctgcgtc gacgcccgcc agcgcgagaa cttccacaag gagatggaag cgcacaacta
     8641 cgtgcggaat tttgaagtca ccatgcgccg caaggatggc acggtgttgc tggcggtaga
     8701 aagctgcttc gccacgcgcg atgccaccgg caacatcgag cgctaccagg gattcgtcct
     8761 cgacatgacg gaaaagcggc gggccgaaga tgagatgagc cggcgcaacc gcgaactgaa
     8821 cgcgctcaac gccatggcgg tggtggcggc gcagtcgttc gacctggacg agatattgaa
     8881 cctgacgctg cgccaggtgg tcacgctgtt cggcgccgag agcggctcgg tttatctctc
     8941 ggacgcagac ggaaccatgt ttcgccggcg cgcggcctgg ggaccgcgca agaccggcaa
     9001 agcgcagccc gcggaaatcg cctttaccga cggctttggc gacctggtga tgcgctcgcg
     9061 cacggaagtg gtgacggcgg agtatctgcc tcacctgccc aaaaatgtca ccgagtttct
     9121 gcgctccgac gctgaccgct cctggatctg ggtgctgttc tggggcaagg acgcgcctgt
     9181 agggatcatg gggttgtgca accaggttgg gcacgaatat tccagcaacg acgagaatct
     9241 gctggtggcg atcagccggc aactggcgac cacgatcgaa aaagtccggc tctacgaaga
     9301 aacctgccgc gcctacgaag acttgcgcaa gacgcaggaa caactgctgc agagcgaaaa
     9361 gatgtcggcc gtggggcagc tcattgccgg cgtcgctcac gagctcaaca atccgctcac
     9421 cgcgatctta ggctatgcgc aactgctcga gagcgaaggt ttgaacaccc gtgcgcagga
     9481 ctacatcacg aagcttttca agcaggcgca gcgcacgcat cgcgtggtgc agaacctgct
     9541 ctcctttgcc cgacaacggc agccacagcg ggaggaagtc gatctgcgca aggtgctgga
     9601 cgaaacgctg gctctgcgcg attacgatct caagaccaac aacatccagg tcgagcgcga
     9661 ggcccaggcc gatcccgcga tggtagtggc cgatccgcac cagatcgagc aagtgtttct
     9721 caacatcatc aacaacgccg tggatgcgat tcttgaaacc ggaggcacgg gcaagctgcg
     9781 aatccgcgtc tacaccgaga acgggcacgt cgcaacccag ttcgctgacg acggcgcggg
     9841 catcaaagac cccaagcgca tttttgatcc gttctacacc accaagagcg tgggcaaagg
     9901 cacgggactg gggctgagca tttgctacgg cattgtgaag gagcacggag gcgacatcac
     9961 ggcgcgcaac gcagccgagg gcggcgcggt gattgaagtc cggctgccga cggccgctcc
    10021 ggttgccgcc cagccggacg ctgcaaagcc ggcgccgcgt ccgtatgagg ccgcggtgca
    10081 cgggcgcatc ctgctggtcg aagaggaaga ggcggtgctc gaattcgagc gcgacgtcct
    10141 caccggcgcc ggcgccacgg tggtgaccgc gcgtactagc cacgacgtga agagcaagct
    10201 gacgtccgat tcgattgagg ccgtcattat ggccggcaag atgccgggcg actggaacgc
    10261 gaaagagtct tacacctgga tcaaagagaa ttgcccggcg atggtcggcc acgttctgtt
    10321 cacgttctcc aacggcgtgg aacagggcga cgggcgcgcg ttcctgcagg agaatggaat
    10381 tccctacctg gtcaaaccct tcgaagtcgc cgaactgatc gcccagaccc ggaagctgct
    10441 gcagaaagcg caggcagcgg gggcaggggc aagctagaag tttatcgacg caagcccgca
    10501 gaccgtgaga tagccggctt tcaatgggta actacgggaa tcggatgtgc acggcattcc
    10561 cgacgatact cacgttgaga tattccccgt agtatttccc caccccgcgc acgagccccg
    10621 ccatcagggc caccaaccca cggttggatt catagtgcat gatgaggtgc
//
LOCUS       DASPUL010000410         5737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1441705, whole genome shotgun sequence.
ACCESSION   DASPUL010000410 DASPUL010000000
VERSION     DASPUL010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441705"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VEJ00_09950"
     CDS             <1..646
                     /locus_tag="VEJ00_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXX01525.1"
                     /translation="GMGESLVNQLIERGLVKNVADIYDLTKKDLLSLDRFAEKSAQNI
                     LDEIESSKKLPLERVIYGLGIRFVGERTAQFLAEHFGSMEALEHASLEELQDVTEVGP
                     RIAESIVEFFSIAANRKLVERLGAAGLAFKGKRKERGTKLASKTFVLTGTLAHFTRDE
                     AKKLIEDAGGKVTGSVSKKTDYVVAGADAGSKLDKAKELGVAVIDEKEMEKLVG"
     gene            complement(1125..1607)
                     /locus_tag="VEJ00_09955"
     CDS             complement(1125..1607)
                     /locus_tag="VEJ00_09955"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HXX01526.1"
                     /translation="MDYLWTPWRYAYVSTTEKTSGCVFCDAAKEKDDEKARIVYRGKL
                     CFVILNTFPYTPGHVMIVPYAHLDELQKLPSDTANEMMALSQRMETVLRELYRPDGIN
                     LGMNIGKAAGAGIAGHIHMHVLPRWVADANFMSVVGETRVLPETLDVTWERMRRALEE
                     "
     gene            1990..3816
                     /locus_tag="VEJ00_09960"
     CDS             1990..3816
                     /locus_tag="VEJ00_09960"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HXX01527.1"
                     /translation="MLDETTFDHANNPDSTATAVTEAESSDQVPAETAAETNSPVRAS
                     DDKPAPAEDFAAALETFTTESEEAVSDDRVIRGTVLKLTPTHVVVDIGAKSEGMLPLA
                     EILDHQGNPKFKPGDEIDVMRDKGEAEEGYINLSHQKAQRIHAWEEIEKAHHEKKPIK
                     ALVIDRIKGGLTVDIHGAHAFLPGSQVDLRPVRNLDAMKGQTIEVAVIKLNKKRGNIV
                     VSRKELLEGEQNEKRSKTLEHLEEDAILTGVVKNLTEYGAFVDLGGLDGLLHITDMSW
                     GRLTHPKDLVNVGDQIQVKVLKYDKDKQRVSLGFKQLTPDPWLDAEHRYPVGAHVSGR
                     VISVTDYGAFVELEQGIEGLVHVSEMTWSKRMKHPSKLVGVGDQVECVVLNVNPAERR
                     ISLGMRQLAANPWDSLHEKFPVGMTVEGRVRNLTDFGAFVEIEDGIDGLVHVSNLSWT
                     KRVKHPSEVLKKGDRVKAIVLAIEPDKRRLSLGMKQLQPDVWESFFASHHIGDVLHGK
                     VLRVAAFGAFVEVAEGVEGLCHKSEAVDGDGQPLHLEPGVEHDFKIIKMSPEEKKVGL
                     SIRAVGEEASRSEVEAYKQPVSSTSSTIGELISWKRASNESN"
     gene            4023..5438
                     /gene="lpdA"
                     /locus_tag="VEJ00_09965"
     CDS             4023..5438
                     /gene="lpdA"
                     /locus_tag="VEJ00_09965"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXX01528.1"
                     /translation="MPETIYDVAIIGSGPAGYTAAIRAGQYGLKTALIEKDGFLGGTC
                     LHVGCIPTKALLFNAELWDHLKDAKEFGIEGVDARKLNWGAIQERKGKIVSKHAKGLE
                     FLMKKNKVDTVKGYGKLTGPAQNGVFTLELTNDGKATHLKTKNVILATGSEARMLPGL
                     EANDKILTNIEILSLKEIPKSLVIVGAGAVGVEFASIYRSFDTEVTIIEMLPRLVPVE
                     DEEVSKELARVFRKRGINFHTSAKVDRVDKTKSGIAVIITVDGKQQKIEAEKILIAVG
                     RKPRTQNIGLEKTKIKPDREFIKTDSWMQTAEPGVYAVGDIVLGTPQLAHVGAMEALV
                     AVAKIAGKPAKPINPEHIPGCTYCHPEIGSVGLTETKARETGYNVKIGKFPFTANSRA
                     SIVGQHEGFIKIVSDADYGEILGVHIIGPQATELIAEAVAAMELEATVEDLMWTIHAH
                     PTLAEAMLDASNSVYGLAINA"
BASE COUNT         1314 a         1813 c         1525 g         1085 t
ORIGIN      
        1 cggcatgggc gaatcgctcg tcaatcagct catcgagcgt ggcttggtca aaaacgttgc
       61 cgacatctac gacctcacca agaaagacct cctgagcctc gatcgattcg ccgaaaaatc
      121 ggcgcagaac attctcgacg aaatcgaaag ctcgaagaag cttccgctcg agcgtgtcat
      181 ctacggcctc ggcatccgct tcgtcggcga gcgcaccgcg cagttcctcg ccgagcactt
      241 cggctccatg gaagccctgg agcacgcgag tcttgaagaa ctacaggacg tcaccgaagt
      301 cggcccgcgc atcgccgaaa gcatcgtcga gttcttcagt atcgccgcca atcgcaaact
      361 ggtcgagcgc ctgggcgcgg ccggtctggc ttttaaaggc aaaaggaaag agcgcggaac
      421 caagctcgcc agcaagacct tcgttttgac gggaaccctg gcgcacttca cgcgcgacga
      481 agccaaaaag ctgatcgagg acgccggcgg aaaggtcacc ggctccgtca gcaagaagac
      541 agattacgtc gtcgctggcg ccgatgccgg ctcaaagctg gacaaggcaa aagaactggg
      601 agttgcggtg atcgacgaaa aagagatgga gaaacttgtc ggctgaggct gtaaaatcgc
      661 accgcgacat atgagtcctg tggaagcttc gcgcaagttc atccagaccg tacgcttcgc
      721 tcttgcggca gcagtcgtag tgtatttgct ggtgactcta cgtctccctt ccgcagcgac
      781 tccgaacccc gtaatgcttc gagccctcgg tgtggtggcc atcacagaaa cgattcttat
      841 ctatgtagta cggagaattc aggttctgcg tgttgaggcc gcgctggaga cggagcctca
      901 ggatccaaag atcttgctcc gctggcgcca gggttacctc attacttact ctctcagcct
      961 ttcgattgct ctatacggat tggtgttgca cttcttggga ttctccctat ctcatattgc
     1021 tccattcttc ctcgctggta tcgcactcat cctgttcctc agcccgaggg cgcttccggg
     1081 cagcacgttg ccgcaatccc ggcctatcat tccccgctga tgggctattc ttctagcgcc
     1141 ctcctcatcc tctcccaagt cacgtcgagc gtctccggca gcacccgtgt ctctcctacc
     1201 accgacataa aattcgcgtc tgcgacccaa cgcggcagca catgcatgtg aatgtgcccc
     1261 gcgatccccg ccccggccgc cttcccgatg ttcatcccca gattgattcc atccggccga
     1321 tacaactccc gcagcacagt ctccatcctc tgcgacaggg ccatcatctc gtttgccgtg
     1381 tcgctcggca gcttctgcaa ttcgtccagg tgggcatacg gcacgatcat cacatgcccc
     1441 ggcgtgtatg gaaacgtatt caggatgaca aagcacaatt tcccgcggta tacgatccgc
     1501 gccttctcgt catccttctc cttcgccgcg tcgcaaaaca cgcacccact ggttttttcc
     1561 gtagtcgaaa cgtaggcgta gcgccagggc gtccacaagt aatccatagc agggaggtta
     1621 gcagatgctc gcgaatcgcg gaaacccgcg cctgagcggc tcgacttttg cgcgtcgcct
     1681 gcaaaattca ctcacttgcg gcgctgatta ctaagagctg attactaatt tccgggtcca
     1741 acccggttca acggttcccg ttcttacacc gttgaaacct gcctgtaaca caacgcggat
     1801 ttaggtttga cactgttcgc aagccgttgc tatagtcgaa gagttcccat tggacgcggg
     1861 ttctctgtaa aaacgtccga cagctgggat tgtccaagtt cacccgccag ggtgggccca
     1921 ccacaaccaa acgactcgtc cagcagtccg cagaacaggc cccgcttccg gcagcagaga
     1981 ggcataacct tgctcgacga gaccacgttc gaccacgcca acaatcccga ttccactgcc
     2041 accgccgtaa ccgaggcaga atcctcggat caagtgcccg cggaaaccgc cgctgagacg
     2101 aacagcccgg tgcgcgcttc cgacgacaag cccgcgcccg ccgaggactt cgccgccgcg
     2161 ctcgaaacct tcaccaccga atccgaagag gctgtgagtg atgatcgcgt catcagaggc
     2221 acggtgttga agttgacccc gacgcacgtg gttgtcgaca ttggcgcgaa atcggaaggc
     2281 atgcttcctc tcgcagaaat tctcgaccac cagggcaacc ccaagttcaa gcctggcgat
     2341 gagatcgatg taatgcgcga caagggcgaa gccgaagaag gctacatcaa cctgtcgcac
     2401 cagaaagcgc agcgcattca cgcctgggaa gagatcgaga aggctcatca cgaaaaaaag
     2461 cccatcaaag ccctcgtgat cgatcgcatc aaaggcgggc tcacagtcga catccacggc
     2521 gcgcacgcgt tcctgcccgg ctcgcaagtc gatctgcgcc cggtccgcaa cctcgatgcc
     2581 atgaagggtc aaaccatcga agttgccgtc atcaagctca ataagaagcg cggcaatatc
     2641 gtggtttcgc gcaaggaact gctcgaaggc gagcagaacg agaagcgctc caagactctc
     2701 gagcatctgg aagaagacgc aatcctcact ggcgtggtca agaatctcac cgagtacggc
     2761 gccttcgtcg atctcggagg cctcgacgga ttgcttcaca tcaccgacat gtcctggggg
     2821 cgcctcactc atcccaagga cctggtcaac gtcggcgacc agatccaggt caaggtcctc
     2881 aagtacgaca aggataagca gcgtgtttcg ctcggcttca agcagcttac gcccgatccc
     2941 tggctcgatg ccgagcatcg ttatcccgtc ggcgctcacg tctcgggccg cgtcatcagc
     3001 gtcaccgact acggagcctt cgttgaactc gagcagggca tcgaaggtct ggttcacgtc
     3061 agcgagatga cctggtccaa gcgcatgaag cacccctcaa agctggtcgg cgtcggcgat
     3121 caggtcgagt gcgtggtgct caatgtcaat cccgcagagc gccgcatctc cctcggcatg
     3181 cgccaactgg ctgccaatcc ctgggattcg ctgcacgaga aattcccggt gggcatgacg
     3241 gtcgaaggtc gtgtccgcaa tctcaccgac ttcggagctt tcgtcgaaat cgaagacggc
     3301 atcgacggct tggttcacgt cagcaacctc agctggacca agcgcgtcaa gcacccgtcg
     3361 gaagtgctga aaaaaggcga tcgagtgaag gcgatcgttc tggccatcga gccggacaag
     3421 cgccgcctct cgctgggcat gaagcagctt cagcctgacg tctgggagtc tttctttgcc
     3481 tcgcaccaca tcggcgacgt tcttcacggc aaggtcctgc gcgtagccgc ctttggcgcg
     3541 tttgtcgaag tcgccgaagg agtcgagggc ctgtgccaca aatccgaagc ggtcgacgga
     3601 gatggccaac ccctgcacct tgagcccggc gtcgagcacg atttcaagat catcaagatg
     3661 agtcccgaag agaagaaggt cggactcagc attcgtgccg tcggggagga agcgtctcgc
     3721 tccgaggtcg aagcctacaa gcagccggtc tcgagcacca gttcgaccat cggcgagctg
     3781 atttcctgga aacgcgccag caacgaaagt aactgaaccg tgttgtcatc ctgagcgaag
     3841 aaacagcctc gcgtttacgc gaggctgttt cgcagtcgaa ggaccccgtg ttcactggcg
     3901 caatcaccgg cgtcgccggg aatttccacc ccgaccccgt cccaagtttt acccccgcca
     3961 ccaccctgtt ctagaatcat tcctttgccc gtcatcatct gtccttacag gagagcactc
     4021 ctttgcccga aaccatctac gacgtcgcca tcatcggcag cggacctgcc gggtacaccg
     4081 ccgccattcg cgccggccag tacggcctga aaactgccct catcgaaaag gacggattcc
     4141 tcggcggcac ttgtctccac gtcggctgca tcccgaccaa ggcactgctg ttcaacgccg
     4201 aactctggga tcacctcaaa gacgccaagg aattcggcat cgagggcgtc gacgcccgca
     4261 agttgaactg gggcgccatc caggaacgca agggaaagat cgtcagcaag cacgccaaag
     4321 gcctcgagtt cctgatgaag aagaacaaag tcgataccgt aaagggctac ggcaaactca
     4381 cagggcccgc gcagaacggc gtatttaccc tggaactgac aaacgatggc aaagccacac
     4441 acctcaagac gaagaacgtc atcctcgcca ccggctccga agcccgcatg ttgcccggcc
     4501 tcgaagccaa cgacaagatc ctcaccaata tcgaaatcct gagcctgaaa gaaatcccga
     4561 aatcgctcgt gatcgtcggc gccggcgccg tcggtgtcga attcgcctca atctaccgct
     4621 cattcgacac cgaagtcacc atcatcgaaa tgctgccgcg cctcgttcct gtcgaagacg
     4681 aagaagtctc caaggaactc gcccgcgtct tccgcaagcg tggcatcaac ttccacacca
     4741 gcgcgaaagt cgacagagta gacaaaacga aatctggaat cgctgtcatc atcaccgtcg
     4801 acggcaagca acaaaagatc gaagccgaaa agatcctgat cgctgtcggc cgcaagccgc
     4861 gcacccagaa catcggcctc gaaaaaacga agatcaagcc cgaccgtgag ttcatcaaaa
     4921 ccgattcctg gatgcagacc gccgagcccg gcgtctacgc cgtcggcgat atcgttctgg
     4981 gcacaccgca actcgcgcac gtcggcgcca tggaagccct ggtcgcggtt gccaagatcg
     5041 ccggcaagcc cgccaagccg atcaaccccg aacacattcc cggatgcacc tactgtcatc
     5101 ccgaaatcgg cagcgttggc ctcaccgaaa ccaaagcccg cgaaaccgga tacaacgtga
     5161 agatcggtaa gttccccttc actgcgaatt cgcgcgccag catcgtcggc cagcacgaag
     5221 gcttcatcaa aattgtcagc gacgccgact acggcgaaat cctcggcgtc cacatcatcg
     5281 gcccgcaggc cacagaactg atcgctgaag cggtagctgc gatggaactg gaagccacgg
     5341 tagaggactt aatgtggacg atccacgccc atcccacgct ggccgaagcc atgctcgacg
     5401 cctcaaacag cgtgtacgga ttggcgatca atgcataggc tgatgaagct cgtagcaggg
     5461 ttagtcggct gtatgttggc tgcgccggcg ctatttctgg gtttttacga tgtgcgtaac
     5521 tttcgaccat acctcccagc gatccggaca atctacgcgg acatggacga gggcgacaga
     5581 aatccaccca acaatgtcca ggatttcgtt ttgaaagttg atggaaggat cgtgggcagc
     5641 ttagacgcga gagacttgct ctcagaactt ggccctcgca tgaatatggg ttcctggcac
     5701 tatcactcga ccatgtggcg gttcctgctt ccgctgc
//
LOCUS       DASPUL010000411         2979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1995321, whole genome shotgun sequence.
ACCESSION   DASPUL010000411 DASPUL010000000
VERSION     DASPUL010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1995321"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VEJ00_09970"
     CDS             <1..651
                     /locus_tag="VEJ00_09970"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXX01529.1"
                     /translation="RVTEYYAKDSTLSKDQIKAQVAKGAAHHMDCVDCHNRPTHIYVS
                     PDQSVDQSLLARRLDVSLPFLKQQAVTVLAATYKTNDEAMRAIASGLQNFYESKYPDM
                     AKSKQLEIRNAVTEIQQIYGRTTFPDMKLNWQTHNNNLGHFYYPGCFRCHDGQHVSAD
                     GKVVSKDCNQCHTVMSEAEGTASLAATPTTSFQHPVELGDLTQVNCSDCHTGGTGP"
     gene            complement(751..2004)
                     /locus_tag="VEJ00_09975"
     CDS             complement(751..2004)
                     /locus_tag="VEJ00_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXX01530.1"
                     /translation="MRKSVGVSLFLILVTFSWNAGLASDLALVHAKIYPSPTEPAIED
                     GTILVHDGRITAIGPSARVKPPRFARAVTVIDCQGKVVTAGFWNSHVHIFTLGLLHAE
                     KLSSRELSSQMEQMFNRWGFTTVFDIASLLDNTNLIRRRIDDREVQGPRILTVGEPFY
                     AKGGTPVYVRGFLEENHIGSAEVESVPQAETRVRQQIRDGADGIKIFAGSIESGSVLI
                     MPADIAKAVVAEAHREGKPVFAHPSNNEGIELSLKSGVDILAHTTPMSGPWTPEQVQR
                     IRAANMALIPTLTLFEVEAKKFGESAEDAANDMRAASQQVKAYSDAGGQILFGTDVGY
                     TDYYDTSEEFSLMSRAGLSFQQILASLTTSPAERFGYSAHSGRIAKGMDADLVVLSSD
                     PAKDVTALSHVAFTIRAGKIIFGKL"
     gene            2264..>2979
                     /locus_tag="VEJ00_09980"
     CDS             2264..>2979
                     /locus_tag="VEJ00_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01531.1"
                     /translation="MKRKFKTMDANEAVAHVAYRVNEVIAIYPITPSSPMGEWADQWA
                     SEGVPNAWGSVPHVIEMQSEGGAAGAVHGALQTGSLATTFTASQGLLLMIPNMNKIAG
                     ELTPTAFHVSARTVATHALSIFGDHSDVMFCRTTGFAMLCSNSVQETMDLALIAHAAS
                     LETRIPFLHFFDGFRTSHEVNKIEMLSEDDMRALIDMDRVIEHRQRALSPDHPVLRGT
                     AQNPDVFFQIRETANRYYDAA"
BASE COUNT          615 a          933 c          856 g          575 t
ORIGIN      
        1 cgggtcaccg agtactacgc caaggattcc acgctgagca aggaccagat caaagctcag
       61 gtggcaaaag gcgcggcgca tcatatggac tgtgtcgact gccacaaccg tcctacgcac
      121 atctacgttt cgccggatca gtcggtcgat cagtcactgc tggcccggcg actcgatgtc
      181 tcgctgccct tcctcaagca gcaggccgtc acggtgctgg ctgcgaccta taaaacgaac
      241 gatgaggcca tgcgagcaat cgccagcggg ttgcagaatt tctacgagag caagtatccc
      301 gacatggcca agagcaagca gctggagatc cgcaatgcgg tcaccgagat tcagcagatc
      361 tacggccgga ccaccttccc cgacatgaaa ctcaactggc agacccataa caacaacctg
      421 gggcacttct actatcccgg ctgcttccgc tgccacgacg gccagcacgt cagcgccgac
      481 ggcaaggtcg tgagcaagga ctgcaatcaa tgccacacgg tcatgtcaga ggcggaaggc
      541 acagccagcc tggccgcaac gccaacgact agcttccagc atccggtcga gctgggagac
      601 ctgacccagg tcaactgcag cgactgccac accggaggaa cagggccgta gtagcgtcga
      661 gcaggaggct tagagccttc tttttctgtt atccacagtt catccccagt tcgcggacga
      721 aaactcctct cccgatgaca tggctttggc ttacagtttg ccgaaaatga ttttgcctgc
      781 ccggatggtg aaagccacat gggaaagggc agtgacgtct tttgcgggat cgctagacag
      841 aacgacgaga tcggcgtcca ttcccttggc aatgcgacct gagtgggcgg aatagccgaa
      901 gcgctctgca ggactggtag ttagcgacgc cagaatctgc tggaaactca gccccgcgcg
      961 cgacatgaga gagaattcct cgctggtgtc gtagtaatcg gtgtagccga catcggtgcc
     1021 aaacaggatc tgcccgccag catcggagta ggccttcact tgctgtgatg ccgcgcgcat
     1081 gtcattggcg gcgtcttcgg cagactcgcc gaattttttc gcctccacct caaagagcgt
     1141 cagcgtcgga atcagcgcca tgttcgccgc cctgatgcgc tgcacttgct ccggagtcca
     1201 gggtccgctc atcggcgtgg tgtgggcgag gatgtcgact ccgcttttga gagatagttc
     1261 aatcccttcg ttgttggagg gatgcgcgaa cactggtttg ccctcccgat gcgcttcggc
     1321 caccacggcc ttggcaatgt cagcgggcat gatcagcacg cttccggatt cgatcgaccc
     1381 ggcaaaaatc ttgatgccgt cggcgccgtc gcgaatctgc tgccgcactc gcgtctcggc
     1441 ctgcggcacc gactccactt cggcgctgcc gatatggttt tcctccagaa aaccgcggac
     1501 gtagaccgga gttccgccct tggcgtagaa cggttcaccc accgtcagaa tgcgcggccc
     1561 ctggacttcc ctatcgtcga tgcgccggcg aatcaggttg gtgttgtcga gcagcgaggc
     1621 gatgtcaaaa accgtagtga agccccagcg gttgaacatc tgttccatct gcgatgacag
     1681 ctcacgggag gacagctttt cggcatgcag caagccgagc gtaaagatgt ggacatggct
     1741 gttccaaaag ccggccgtga ccaccttccc ctggcagtcg atgaccgtga cggcgcgcgc
     1801 gaaacgcggc ggctttaccc tggcgctcgg accgatggct gtgattcgcc cgtcgtgcac
     1861 gaggatggtg ccgtcctcga tggccggttc cgtcggcgag ggatagatct tcgcatgaac
     1921 cagcgccaag tctgatgcca ggcccgcatt ccaggaaaag gtaaccagaa tcaggaaaag
     1981 tgacacgccc acgctcttgc gcatgcggtt ccatacgcta atggtcggga ctttgttccg
     2041 ctagcgagtt tacggcgtcg ctgagaacgg tttgccacaa aacactggcg tccttttggt
     2101 ccccaacgga agtcgcatcc cagcaatgcc ttacgggccg attgtgattc tgatcacacc
     2161 gctgtgtgaa cccacgtccc gacatcccct gttccatccg gcatcctgaa gacatcacgg
     2221 gggttaccca gtggccgctg tgagactcca gatgggggat gtcatgaagc gcaaattcaa
     2281 gaccatggac gccaatgagg cggtggcgca tgtcgcgtac cgcgtgaatg aagtgattgc
     2341 catctacccg atcaccccgt cctccccgat gggggaatgg gccgaccagt gggcgtcaga
     2401 gggcgtaccc aacgcctggg gcagcgtgcc ccacgtaatt gagatgcaga gcgaaggcgg
     2461 cgccgccggg gctgtgcatg gcgccctgca aaccggatcg ctggccacga ctttcacggc
     2521 atcgcagggc ctgctgctga tgatccccaa catgaacaag attgccggcg agctgacgcc
     2581 cacggcattt cacgtttccg cccgtaccgt ggctacgcac gcattgtcga ttttcggcga
     2641 ccactccgac gtgatgtttt gccgcaccac cggcttcgcc atgctctgct cgaactccgt
     2701 gcaggaaacc atggacctgg ccctgatcgc gcacgccgcc tcgctcgaga cccgcattcc
     2761 cttcctgcac ttcttcgacg gcttccgcac ttcgcacgaa gtcaacaaga tcgagatgct
     2821 cagcgaagac gatatgcgcg ccctcatcga catggaccgc gtcatcgagc accgccagcg
     2881 cgcactctcg cccgatcatc cggtgctgcg cggcacggcg cagaatccgg acgttttctt
     2941 ccagattcgc gaaactgcga accgctacta cgatgccgc
//
LOCUS       DASPUL010000412         4443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_908450, whole genome shotgun sequence.
ACCESSION   DASPUL010000412 DASPUL010000000
VERSION     DASPUL010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908450"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VEJ00_09985"
     CDS             <1..486
                     /locus_tag="VEJ00_09985"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HXX01532.1"
                     /translation="LQLFAPGVETEVVPFDQIIPQVLEGKYEAGLIIHEGQLTYDKSG
                     LHRIVDLGRWWQKVTGLPLPLGGNAIRRALGPQLMTAVTAALRESIQYALDHREEALS
                     YAMQFARDLDTATADKFVGMYVNERTLDYGPDGREAVRRLLDMGHKAGIIGPEAKVEW
                     V"
     gene            629..1321
                     /locus_tag="VEJ00_09990"
     CDS             629..1321
                     /locus_tag="VEJ00_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXX01533.1"
                     /translation="MLSQTLLIDADDTLWENNIYFERAIERFISFLNHHEFSPEQVRE
                     VLNEVERECIVKHGYGMHSFAHALVDTFERLSPQPVTPELHSQILSFAHRIADHPIEF
                     LPEVPETLQYLSARHRIILVTKGAVPEQTGKIERSGVKNQFAAVEIVAEKSATTYAHL
                     IEKYNLARRATWMVGNSPKSDINPALAAGLHAVFVPHGDTWILEHEEVNPAPAGQKLL
                     IVGRFAELREHF"
     gene            1488..2738
                     /locus_tag="VEJ00_09995"
     CDS             1488..2738
                     /locus_tag="VEJ00_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXX01534.1"
                     /translation="MAIQTQNFRRLLHTVEALSELGPALAVEREFSESARLMLASVME
                     AAGAREGVLFLFNEKPAMLTSAAAEGYALMPDPGFIPLLPKHVHALTAARGPIVLNSS
                     TYSVFLSSNGNVAPELFKCIAPLKAAGRLAGVIALGRRPGDALYEDSELDALQLLCGY
                     VALAVQNYALTQSIAQRVSENLRLMASLHGFYDNALEAFATAIDVKHVNIHGHSLRVG
                     RYAAAIGEAMGMEPGEVAALRSAGYLHDIGKVAVDRRLFGKPAALDPEEFREMADHTT
                     VGHQIVSAVQFPWPKIPEAVRWHHERSDGSGYPDRLGLGDLPVTVRIVGIADSFDAMT
                     SARPYRAPMSVGSALSDLVRMTPEKFDPNVVQGLLIQVRRDAVGSNRTPLLQGMSVNI
                     AATDIDQLAATLQHKVSRGKKYLT"
     gene            2975..>4443
                     /locus_tag="VEJ00_10000"
     CDS             2975..>4443
                     /locus_tag="VEJ00_10000"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HXX01535.1"
                     /translation="MKTGPGKLALFLLCLSLVLAVPQVLAKKKDQTPTPTQDERKRAV
                     HALNRLAFGPRPGDVEKVQAMGVERWIDLQLHPEKIPDTDAETRLAPLRTLRMSSHEI
                     AEEFPDGALVKQVLDGKKPMPSDPARRAVYQVQIARLQQKTQQREQEKQAKVEPTAPP
                     KPAPAPAMTEPPATETAKTQEELAAAAAATPETSPGMLDNSVNATSAAGKAPSNATAR
                     DDGSRNFAPTTNPAADDGDAKRLEDRLYVDLKVQSLVELPPAERYRKVMAMSPDEQLA
                     FADQQRGGKGQDFLAGLEPRQKETLQAMNNPQSVVTDELMQAKVLRAIYSERQLEEVM
                     TDFWFNHFNVFIGKGADRVLITSYEQNVIRPHALGKFEDLLVATAQSPAMLFYLDNWL
                     SVGPNSMQGLGIPAHPGRNGPYRYPPRNPNGKARPNSGLNENYGRELLELHTLSVNGG
                     YSQRDVTEVAKVFTGWTIDKPNEGGSFQFDPRKHEPGPK"
BASE COUNT          914 a         1419 c         1292 g          818 t
ORIGIN      
        1 ctgcaacttt ttgcgcccgg agttgagacc gaagtcgttc cgttcgacca gatcattccg
       61 caagtgctcg aaggaaaata tgaggccggg ctgatcatcc acgaaggaca gctcacatac
      121 gacaaatccg gattgcaccg catcgtcgat ctcggccgct ggtggcagaa agtcaccgga
      181 ttgcctctgc cgctgggagg caacgcgatt cggcgcgcgc ttggtccgca actgatgacc
      241 gccgtgaccg cggcgctgcg cgagagtatc cagtacgcgc tcgatcatcg cgaagaggct
      301 ttgtcctacg cgatgcagtt cgcccgcgat ctcgatacgg ccaccgccga caaattcgtc
      361 ggcatgtacg tcaacgagcg tacgctcgac tacggccccg acggccgcga agccgttcgc
      421 cgcctgctcg acatgggaca caaagcaggc atcatcgggc cggaagcgaa agtggagtgg
      481 gtgtgaagaa agcttctagc tgctagctcc tagcttctag cttgtgaccg gaaaatcgaa
      541 ggttttcctt cttgtcttca ccccattcgg aaactatcag ccggacacga ttcgcttagc
      601 cagaagctag cggctaggag ctagaagctt gctttcccaa accctcctca tcgacgccga
      661 cgacacgctc tgggagaaca acatctactt cgagcgcgcg atcgagcgct tcatttcatt
      721 cctgaaccac cacgaattct ctcccgagca ggtgcgcgaa gttctcaacg aagtcgagcg
      781 cgaatgcatt gtgaagcatg gatacgggat gcacagcttc gcccacgcct tggtcgacac
      841 gttcgaacgc ctgagcccgc agccggtcac gcccgagctg cactcgcaga ttctcagttt
      901 cgcgcaccgc attgccgatc atcccattga attcctgccg gaagtgcctg agaccttgca
      961 gtacctgtca gcacgccatc gcatcattct cgtcacgaag ggagctgttc ccgagcaaac
     1021 gggaaaaatc gagcgctccg gggtgaagaa ccaattcgct gcggtcgaaa tcgtcgcgga
     1081 gaagagcgcc acgacgtacg ctcacctgat cgaaaaatac aatcttgccc gccgcgcaac
     1141 ctggatggtc gggaacagcc cgaagtccga catcaatccc gcgctggccg ccgggctgca
     1201 cgccgtcttc gtcccccacg gcgacacctg gatcctggag cacgaggaag tcaatcctgc
     1261 gcctgcaggg cagaagctgc tgatcgtcgg ccgcttcgcc gaactgcgag agcatttcta
     1321 gtttgtggca tgcttcggtc aatagcgtgc tgacagcaga atcatcttcg tcacccattt
     1381 tcgtcatcgt agccaccgca tcaaactaac caacaaccct ctctcgccct cacagtttcc
     1441 tgcgtgttac gctcaacttg ctctgcgatc tggctcccac ttcgtcagtg gcgattcaga
     1501 cccaaaattt ccggcggttg ctgcacaccg ttgaggcgct ctcggagctc ggccccgcgc
     1561 tggcggtgga gcgcgagttt tcggaatctg cccgcctcat gcttgcctcc gtcatggagg
     1621 ctgcgggagc gcgcgagggc gtcctctttc tctttaacga gaaaccggcc atgctgactt
     1681 cggctgcggc cgagggttac gctctgatgc ccgacccggg cttcatccct cttctgccca
     1741 agcatgtgca cgcgctgacg gcggcgcgtg gacccatcgt cctgaactct tcaacctatt
     1801 ccgtgtttct gagctccaac ggcaacgtcg cgccggagct gttcaagtgc atcgcgcccc
     1861 tgaaagcggc gggccgtctg gccggagtca tcgctctcgg ccgccgaccc ggggacgcgc
     1921 tctacgaaga cagcgagctc gacgccctgc aactgctttg cggctacgtt gcgctcgcgg
     1981 tgcaaaacta cgcgctgacc cagagcattg cgcagcgggt ttccgaaaat ctgcgactca
     2041 tggcgtcgct gcacggcttt tacgacaatg cgctcgaagc cttcgccacc gcgatcgacg
     2101 tcaagcacgt caacattcat gggcactcgt tgcgggtcgg ccgttatgcc gccgcgatcg
     2161 gcgaggccat ggggatggag cccggcgagg tcgctgcttt gcgcagcgcc ggatatcttc
     2221 atgacatcgg aaaagttgcc gtggatcgcc gcctgtttgg caagcccgct gcgctcgatc
     2281 cggaagaatt ccgcgagatg gccgaccaca ccaccgtggg ccatcagatc gtgagcgctg
     2341 tgcagtttcc ctggccgaag attcctgagg cggtgcgctg gcaccacgag cgcagcgacg
     2401 gctccggcta tcccgatcgg ctcgggttgg gcgatctgcc cgtgacggtt cgcatcgtgg
     2461 gcattgccga ttcgttcgat gctatgacca gcgcgcgtcc gtatcgcgct cccatgtcgg
     2521 tcggcagcgc cctgagcgac ctggtacgca tgactcctga aaagtttgac ccgaacgtgg
     2581 tgcagggctt gctgattcag gtgcgccgcg acgccgtcgg cagcaaccgt acgccactgc
     2641 ttcaaggcat gtcggtgaac atcgcggcaa cggacatcga tcaactcgcg gcgacgctgc
     2701 aacacaaagt gtcacgggga aagaaatatc tgacgtaagg tcccctcacc aaaatcgtac
     2761 cagcacttct ttgcgtcctt ggcgggcgtt cttcgcgaac ttcgcggtct aggcttctct
     2821 ttagggccgc aagcgcaggt cggttgccag ctgcattact gtaagtcact ccgccagaat
     2881 gacttacaga tgatcctgaa ccaccgccgc cagcaacttt ttctccgtcc ggggttcata
     2941 atggagagag tctggttctt tcccgggagt cttcatgaaa accggccctg gtaagctcgc
     3001 gctgttcctg ctgtgcctga gcctggtttt agcggtaccg caagttctcg ccaagaaaaa
     3061 agaccagacc cccactccca ctcaggacga gcgcaagcgc gcggtgcacg cgctcaatcg
     3121 ccttgccttc ggccctcggc cgggcgacgt ggagaaagta caggcgatgg gcgtggagcg
     3181 ctggatcgat ctgcaacttc acccggaaaa gattcctgac accgacgcag agactcgcct
     3241 tgctccgttg cgaaccttgc gcatgagttc acacgaaatc gcggaagagt tccccgacgg
     3301 cgcgctggtc aagcaggtcc tggatggcaa gaagcccatg ccgtcggatc ccgccaggcg
     3361 cgcggtgtat caggtgcaga tcgcgcggct gcagcagaag acgcagcaga gagagcagga
     3421 gaagcaggca aaagtggaac cgacagcgcc tccgaagccg gctccagctc cggcgatgac
     3481 cgagccgccc gcgacggaaa ccgcgaagac tcaggaagaa ctcgcggcgg ccgctgctgc
     3541 cactccggag acttcgcccg gcatgctaga caactccgtg aatgcgacaa gcgccgcggg
     3601 caaagcgccc tcgaatgcca cggcaaggga cgacggatcc cggaatttcg ctccaaccac
     3661 gaatcctgcc gcggacgatg gcgacgcaaa gcgcctcgaa gaccgcctct acgttgatct
     3721 gaaagtgcag agcctcgtcg agcttcctcc cgcagagcga tacaggaaag tcatggccat
     3781 gtcgcccgac gaacaactcg ccttcgccga ccagcagcgc ggcggcaaag ggcaagactt
     3841 cctcgccgga ctcgagccga ggcagaaaga gacgctgcag gcgatgaaca atccgcagag
     3901 tgttgtcacc gacgaactca tgcaggccaa ggtgctgcgc gccatctaca gcgagcgcca
     3961 gctcgaggaa gtgatgaccg acttctggtt caaccacttc aatgtgttca tcggcaaagg
     4021 cgccgaccgc gtactgatca ccagctacga gcagaacgtg atccgcccgc atgcgctggg
     4081 caagttcgag gatctgctgg tggcgaccgc acagagcccc gccatgctgt tctatctcga
     4141 caactggctc agcgtagggc cgaactcgat gcaggggctc ggcattccgg cgcacccggg
     4201 gcgcaatggg ccctaccgtt acccgccgcg gaatccgaac gggaaggccc gccccaacag
     4261 cggactgaat gaaaattacg gccgcgagtt gctggaactg cacacgctca gcgtgaacgg
     4321 cggctattcg cagcgcgatg tcaccgaagt cgccaaggtg ttcaccggct ggaccattga
     4381 caagcccaac gagggcggca gcttccagtt cgatccgcgc aagcacgagc cgggaccgaa
     4441 gtt
//
LOCUS       DASPUL010000413         3721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1170652, whole genome shotgun sequence.
ACCESSION   DASPUL010000413 DASPUL010000000
VERSION     DASPUL010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170652"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VEJ00_10005"
     CDS             complement(<1..558)
                     /locus_tag="VEJ00_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXX01536.1"
                     /translation="MLKGAEIVLQCLRAEGVELMFGYPGGAIMPLYDALEDSGIRHVL
                     TRHEQGAVFAAEGYARVTGKVGVAIATSGPGATNLVTGIADAKMDSVPLVCITGQVRS
                     PLIGSDAFQETDVFGVTLSLTKWSRLVRTIDEIPEVIAEGFHWARSGRPGPVVIDIPT
                     DMLKAKKEFSGPVKFTPHARPAEARA"
     gene            complement(551..1600)
                     /gene="ilvC"
                     /locus_tag="VEJ00_10010"
     CDS             complement(551..1600)
                     /gene="ilvC"
                     /locus_tag="VEJ00_10010"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HXX01537.1"
                     /translation="MATIFYESDAKPEALKNKTIAILGYGSQGHAQAQNLRDSGYKVI
                     VGLESSRSSAKQARADGMVVAAPDEAAKRADWIHVLTPDETQAAVYDQYIKPYLHPGK
                     ILGFSHGFSIHFKTIVPPTDVDVVMIAPKSPGHLLRRVYSEGRGVPSLLAIQQDASGR
                     ARELALAYAHGIGSTRAGVLQTTFKEETESDLFGEQAVLCGGLTSLIKKGFETLVEAG
                     YAPEIAYFECLHEMKLIVDLIYEGGLGRMRYSISNTAEYGDLTRGEKVVGDETRAAMK
                     KILADIQSGEFAKEWIAENKNGGHKFNALRENEKRHPIEIVGAELRGMMSWLHKEGKK
                     PADAPKESEKQVVNA"
     gene            2291..3166
                     /gene="ppk2"
                     /locus_tag="VEJ00_10015"
     CDS             2291..3166
                     /gene="ppk2"
                     /locus_tag="VEJ00_10015"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HXX01538.1"
                     /translation="MSKQQKTQFGTSPATDKPQSPSPADGKGSKQLRLGKDCDYDQEL
                     RTLQIELVKLQEWIRHKGLRVVVLFEGRDAAGKGGAIQRIIQTLNPRVCRVVALGTPT
                     EREKTQWYFQRYVAHLPAAGEMVLFDRSWYNRAGVEHVMGFCSESEYLEFLQSCPEFE
                     RMLVRSGIILMKYWFSVSDEEQERRFQDRMNNPTKRWKLSPMDLESRRRWAEYSRAKD
                     EMFAVTDIKQSPWYVVNAEDKKCARLNVIHHLLSMIPYKDLTPEPLKLPPLDKTKYIR
                     PPIQEQNFIPEVYRR"
     gene            3184..>3721
                     /locus_tag="VEJ00_10020"
     CDS             3184..>3721
                     /locus_tag="VEJ00_10020"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXX01539.1"
                     /translation="MPDYLPCHAMAREALVDSRQEILRTAARLFQQRGYDATSMNDVA
                     AALKLSKGGLYHHFQSKDEILFEIMNHAMEITEERVLAPVRVIPDPEDRLRALIRLHI
                     GIVLSPADREITVMLHENHPLPPSLRRRINTRKKEYVHFVEELIREVQKARNSRGAVS
                     PRAAAFALLGMINWIYQWY"
BASE COUNT          740 a         1214 c          995 g          772 t
ORIGIN      
        1 ggccctggct tcggccggcc gcgcatgggg cgtaaacttc accggaccgg agaattcctt
       61 cttcgccttc agcatgtcgg tgggaatgtc gatcacgacc ggcccgggac gcccactgcg
      121 cgcccagtga aatccctcgg cgatcacctc gggaatctcg tcgatcgtac ggaccagccg
      181 gctccacttc gtcagcgaga gcgtgacccc gaagacatcc gtctcctgga aggcgtcgct
      241 cccaatcagc gggctgcgca cctgtccggt aatgcaaacc agcggaacgg aatccatctt
      301 ggcgtcggcg atgccggtta ccagattggt ggctcccgga ccgctggttg caatcgccac
      361 gcccacctta cccgtgaccc gcgcatagcc ttccgccgca aaaactgcgc cctgctcgtg
      421 ccgtgtcagc acgtgccgaa tgccgctgtc ctcgagcgcg tcatacagcg gcatgattgc
      481 cccgccggga tagccgaaca tcagctcgac cccctcggca cgcaggcact gcaaaacgat
      541 ttcggcccct ttaagcattc accacctgtt tttcggattc cttgggtgca tctgccggct
      601 tcttgccttc cttgtgcagc cagctcatca tgccgcgcag ttcggcaccc acgatctcga
      661 tcgggtgccg cttttcgttt tcccgaagag cgttaaactt gtgcccgccg ttcttgttct
      721 cggcgatcca ctccttggcg aactcgccgc tctggatgtc ggccaaaatc ttcttcatcg
      781 ccgcgcgcgt ctcatcgccc acaaccttct cgccgcgggt caggtcgccg tattccgctg
      841 tgttcgaaat ggagtagcgc atgcggccca atccgccttc gtagatcagg tcaacgatca
      901 gcttcatctc gtgcaggcac tcgaaatacg cgatctccgg cgcatatccg gcttcgacca
      961 gggtttcaaa accctttttg atcagcgacg tcagcccgcc acagagcacc gcctgttcgc
     1021 cgaacaggtc gctttcggtt tcttccttga aggtcgtctg cagaacgccc gcccgtgttg
     1081 agccgattcc gtgcgcatac gccagcgcca gttcgcgcgc ccgtccgctc gcatcctgct
     1141 ggatcgccag cagcgatggc acaccgcgtc cttcgctgta cacccgccgc agcagatgtc
     1201 cggggctctt gggagcgatc atcaccacgt cgacgtcggt gggaggaacg atggtcttga
     1261 aatgaatgct gaatccgtgc gagaagccga gaatttttcc ggggtgtaag tatggcttga
     1321 tgtactggtc gtagaccgcc gcctgggtct cgtccggagt gaggacatga atccagtcgg
     1381 cgcgcttggc ggcctcatcg ggagcggcca cgaccatccc gtcggcgcgc gcctgcttgg
     1441 cgctggaacg gctggattcg aggcccacga tgaccttata tccgctgtcg cgcagattct
     1501 gcgcctgcgc atgcccctgg ctgccgtagc ccagaatcgc gatcgtcttg ttcttcagcg
     1561 cctcaggttt ggcgtcgctt tcataaaaaa tagtagccac tcgcgtgctc ctctcgtgtc
     1621 actgatcttg tttcccaacg aaaaacattc cccagaaggc ttgagagctc gatccaccgt
     1681 ctgcaatcag aagctgggga ttcagagacc gaccggtcgg tcggttcatc ctgagatcgt
     1741 aactttggca ccttcctctg tcaagggaaa acaggccctg ccccggtgcc cgccatttat
     1801 agatcgtatt catcgtaagt cactgtagat aaataacttg ctgaactgtc gaagccggta
     1861 aatccgtccc ctctctgaag ggattggccg ataaatcaaa aacccaccgc catctctctg
     1921 gcggtgggtt tcggaaagcc taaactctaa tctcgcttaa ccgtttcctc ccagccagag
     1981 ccttgcggga agcagtcgta cccctacgac tactacgcta actcgaattg cgctccggct
     2041 gtggctcacg gctgccataa cgtgaggcag actgtatcag gcaaacaatc cagtgtcaat
     2101 acactttcgg cttcggcgtt taccatcggt gtattccttt cgaatcaacg actttgtcgt
     2161 gtctcccggg tcgcaaaagc actgaagatt ccactccatc ttccgatttg aagtaagggg
     2221 cgtcgtttcg cctgtacaat tctgaaatct gccctccaag agcgagtttg ccccacaggg
     2281 agatggccgg gtgtccaaac agcaaaagac tcaattcggt accagcccgg caacagacaa
     2341 gccgcagtcc ccctccccag cggatggcaa gggatccaaa caacttcggc tcgggaaaga
     2401 ctgcgactac gaccaggaac tccgcacgct gcagatcgag cttgtcaaat tgcaggaatg
     2461 gatccgccac aaaggcttgc gggtcgtggt gctcttcgaa ggccgcgacg ctgcaggcaa
     2521 gggcggcgcc atccagcgca tcatccagac cctgaatccc cgcgtctgcc gggttgtggc
     2581 tctgggcacg ccgaccgagc gcgagaaaac gcaatggtac tttcagcgct acgtggctca
     2641 cctgcccgcc gccggcgaaa tggttctctt cgaccgcagc tggtacaacc gcgccggggt
     2701 cgagcacgtc atgggattct gctccgaatc cgaatacctc gagttccttc agagctgccc
     2761 cgagttcgag cgcatgctgg tgcgctccgg catcattctg atgaagtatt ggttttccgt
     2821 cagcgacgaa gagcaggaac gccgctttca ggatcgcatg aacaatccca ccaagcgctg
     2881 gaagctcagc cccatggacc tggaatcccg ccggcgctgg gccgaatatt cccgcgccaa
     2941 agatgaaatg ttcgccgtca ccgatatcaa gcaatccccg tggtacgtgg taaacgcgga
     3001 agataagaag tgcgcccggc tgaacgtcat ccatcacctg ctttctatga ttccctacaa
     3061 agacctgact cccgagccgc tcaaactccc tccgctcgat aaaaccaagt acattcgccc
     3121 gcccatccag gagcagaact tcatccctga agtctaccgt cgctaggcgg tgttggcttt
     3181 cgcgtgcccg actatttacc atgtcacgcg atggcccgcg aagccctggt cgattcccgg
     3241 caggagatcc tgcgcaccgc cgcccgcctg ttccagcagc gcggctatga cgccacgtcc
     3301 atgaacgatg tggcggccgc tctcaaactc agcaagggcg gcctctatca ccattttcag
     3361 agcaaggacg aaatcctgtt cgaaatcatg aaccacgcca tggagatcac cgaggagcgc
     3421 gtgctggctc ccgtccgtgt cattcccgac cctgaagacc gcctgcgcgc gctcatccgt
     3481 ctgcacatcg ggatcgtgct cagccccgct gaccgcgaaa tcacggtcat gctgcatgaa
     3541 aaccatcccc tgccgccctc gcttcgcagg cggatcaaca cccgcaagaa ggagtacgtg
     3601 catttcgtgg aggagctgat ccgtgaggtg cagaaggcac gcaattccag gggcgcggtc
     3661 agcccgcgcg ccgccgcctt cgccctgctc ggcatgatca actggatcta ccagtggtac
     3721 a
//
LOCUS       DASPUL010000414         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_216975, whole genome shotgun sequence.
ACCESSION   DASPUL010000414 DASPUL010000000
VERSION     DASPUL010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216975"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VEJ00_10025"
     CDS             <1..804
                     /locus_tag="VEJ00_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXX01540.1"
                     /translation="QALQQKFGLDLRVAMPRDWRIEMKDLERLVDKKTRLIEISLVAM
                     HNGFQYDLKAVCDLAHAHGAYVYADIAQAAGCTPIDVRTSGVDFCACSSFKWLMADFG
                     LGFLFVKEDLLDRVVKRTDYGYFQAETLDSHFLPGDQPGPTPYTFTLGSDASGHFETG
                     THAIGMAHVLAQSLPYIRKLGVDNIQAHRQPLLKRLHQEMPRLGFEPLTSSESTSALA
                     SFAVKDYKAVRERLQKAGVNVRVSPHYIRVSPSVFNDMKDIDKLLEALA"
     gene            801..2144
                     /locus_tag="VEJ00_10030"
     CDS             801..2144
                     /locus_tag="VEJ00_10030"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXX01541.1"
                     /translation="MKPAPTWKLCLSILTIWTCLPVLGQQRSVVIHAGRLIDTSHKQV
                     RQQVSIIVEGARIATVQDGFASPKDAEVIDLSNATVLPGLIDCHKHLTMHLGSGSHFQ
                     DLVTETTADSAFYATANARTTLLNGFTSVRDVGARDTVDIALKHAIEKNLVIGPRMWA
                     AGQIIGPTGGHSDDANGVAQGISSPEWSASIVDSADQARKVVREHRRSGTDLIKIVPS
                     GGVGSVGDDPKLQLMTNDEIKAVIETAHSLGMKVAAHAHGKAAIDNSVRLGIDSIEHG
                     TYADAESFALMKEHGTYLVPTVFVAYSLLQTAKEHPERLPPHIVAKIQVITPIIQAMF
                     TNAHRAGVKIAMGTDSFGDFRGGLTPAKELTEMVRLGMTPMDALAAATVNAADLIGDS
                     QDIGSIEAGKFADIVAVSGDPLKDITELERVAFVMKGGVVYKANGKEVATVLATK"
     gene            complement(2518..2964)
                     /locus_tag="VEJ00_10035"
     CDS             complement(2518..2964)
                     /locus_tag="VEJ00_10035"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXX01542.1"
                     /translation="MARVRGTSYETTIQVRDCCLCLHVQRAARALARRFDNALRPLGL
                     TNGQFSLMMSLNRPEAPAIGAVASLLAMDRTTLTAALKPLQRRGLIKVTTDYDDHRAR
                     LLKLTPQGRKLLARAVPVWRSTHAAVEARLGEGEPDRLRKNLRVLS"
     gene            3106..3363
                     /locus_tag="VEJ00_10040"
     CDS             3106..3363
                     /locus_tag="VEJ00_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01543.1"
                     /translation="MNSTRFAAVAVLVFAFSAVFFGDKEGNAGDSHLMFWFPQGEHMD
                     WGADASDSPVNLHQYTPQPITEFTISVAKWSDGTVASAYQR"
BASE COUNT          756 a          993 c          958 g          693 t
ORIGIN      
        1 caggcgctgc agcaaaagtt cggcctggat cttcgcgtgg ccatgccgcg cgactggcgc
       61 attgaaatga aggatcttga gcggttggtc gacaagaaaa ccaggctgat cgaaatctct
      121 ctcgtcgcca tgcacaacgg cttccagtac gatctgaaag ctgtctgcga ccttgcccac
      181 gcccacggcg cctatgtcta cgccgatatt gcccaggccg cggggtgtac tccgatcgat
      241 gttcggacca gcggagtcga tttctgcgcc tgttccagct tcaagtggct gatggctgat
      301 ttcggcctcg ggttcctgtt cgtgaaggaa gatttgctgg accgggtcgt caagcgcacc
      361 gactacgggt actttcaggc ggagacactt gattcgcact ttcttcccgg cgatcagcct
      421 ggccccacgc cctacacgtt cacgctgggg tctgatgcct ccggtcattt tgaaaccggc
      481 acccacgcta tcggcatggc ccacgtgctc gcgcaatcgc tgccctacat ccggaagctc
      541 ggcgtagaca atatccaggc tcaccgccag ccccttctta aacgtcttca ccaggagatg
      601 ccgcgcctgg ggttcgagcc gctcacttct tcagaatcga cctcggcgct cgccagcttc
      661 gccgtcaagg attacaaagc ggtgcgggag cggttgcaaa aagcgggagt caacgtgcgc
      721 gtttctccgc actacattcg ggtctcgccc tctgtgttca acgacatgaa ggacatcgac
      781 aaactgctgg aggcgttggc atgaaaccag cgccgacatg gaaactctgt ctctccattc
      841 tcactatttg gacctgcctt ccagtccttg ggcaacaaag gtccgtcgtc attcatgccg
      901 gacgcctgat cgacacctcg cacaagcagg ttcgccagca ggtctcgatc atcgtcgagg
      961 gcgccaggat agccacagtt caagatggct ttgcgtcacc caaagacgcc gaggtcatcg
     1021 atctgtccaa tgctacggtg ttgcccggcc tcatcgactg tcacaagcac ctcacgatgc
     1081 atttgggaag cggcagccac tttcaggatc tggttacaga gaccactgcc gattccgctt
     1141 tctacgccac cgcgaacgct cggaccacgc tgcttaatgg tttcaccagt gtccgcgacg
     1201 ttggagcgcg agacaccgtg gatatcgccc tgaaacatgc catcgaaaag aatctcgtga
     1261 tcggtccgcg aatgtgggcc gctggccaga tcatcggccc taccggcggt cattcagacg
     1321 atgccaatgg cgtcgcccag ggaatttcca gcccggaatg gagcgcgtcg atcgtcgaca
     1381 gcgccgatca ggcgcgcaag gtcgtgcgcg agcatcgccg gagcggaacg gatctgatca
     1441 agatcgttcc cagcggcggc gtcggttcgg tcggcgacga ccccaagctc cagctgatga
     1501 ccaacgacga aatcaaggcc gtgatcgaaa ccgcgcactc cctcggaatg aaagtggccg
     1561 cgcatgcaca cggcaaagcg gcgatcgaca acagtgtccg ccttgggatc gattcgattg
     1621 aacacgggac ctacgccgac gccgaatcct tcgctttgat gaaagaacat ggaacctatc
     1681 tggttcccac agtttttgtc gcctactcgc tcctgcaaac ggccaaagag catccggaaa
     1741 ggttgccgcc gcacatcgtg gcgaaaatcc aggtcatcac gccgatcatt caggcgatgt
     1801 tcaccaacgc ccatcgcgcc ggcgtaaaaa tcgcgatggg cacggactca ttcggcgact
     1861 tccgcggtgg cctgacgccg gcaaaggaac tgaccgagat ggtgcggctg ggaatgactc
     1921 cgatggacgc gctggctgcg gcgacagtca atgcggccga tctgatcgga gattcccagg
     1981 acataggctc gattgaagcc ggcaagttcg ccgacatcgt tgcggtctca ggagatcctc
     2041 tgaaggacat caccgagctg gaacgagtcg cattcgtcat gaagggcggc gttgtctaca
     2101 aggcaaatgg gaaggaagtc gcaactgtac tcgcgacaaa atgaccgatc gaagcgaagt
     2161 gtaatcgtgc aactcttgag agttaatcga cggttcaccc tatgaccgga ggcatgtatt
     2221 ctccgggctt tatcaaactc ccgggttcga acgtagcgaa cgaccagtcc gatgtgtctt
     2281 aagtgtcctc tgtcgaaaca tcacgaaccc tcaccaaacc gcatcgtcgt gcagccgaag
     2341 agtcgatgag agaatggttc tgaaacgaat cgataacttt cgcagaaccg ggccggcgct
     2401 ctcaaactcc gcccggtatc tcgcaagatc aacgtttcag gaagtgtgac ttgactagtg
     2461 ggatcgatga gcgaggaaat tcatggtgtt ggctggcaga ttctagcgca ccggcgttca
     2521 ggagagcacg cgtaagttct tccgcagacg atcgggttcg ccttcaccta gacgagcctc
     2581 aactgcagca tgggtactcc tccacactgg gacggcgcgc gcgagcaact tccgcccttg
     2641 cggagtcaac ttcagcagac gagcacggtg atcgtcatag tctgtcgtga ccttgatcag
     2701 gccacggcgt tgcagcggtt tcagagccgc agtcaaagtc gtacgatcca tcgccagcaa
     2761 tgatgccacg gccccaattg cgggggcctc ggggcggttc aaagacatca ttaacgagaa
     2821 ttgcccgttg gtcaggccga gcggacgcaa tgcgttgtcg aagcgtcgcg ccagcgctcg
     2881 cgctgcacgc tgcacatgca ggcaaaggca acaatcacgg acctggattg ttgtttcata
     2941 agacgtgcct cgaacccttg ccatatgcgt attaatgata aatccttgcc gtattctatg
     3001 ctatagttga tatcaaccta ttgtgggttg gggacgggaa aaaagaaaaa gcgaatgagc
     3061 tgccccatgc ggtggcgcag cgcccgtgac catgtagaga aacatatgaa ctcaactcga
     3121 ttcgccgcgg ttgctgtcct ggtattcgcc ttcagcgcgg ttttttttgg cgacaaagag
     3181 ggcaacgccg gagactccca tctaatgttc tggtttcccc agggagagca tatggattgg
     3241 ggcgcggatg cgtctgactc cccggtgaac ctgcatcaat acactcccca gccgatcacg
     3301 gagttcacga tttcggtggc aaagtggtcg gatggaacgg ttgccagcgc gtatcagcgc
     3361 tgaggaacac aatgcagacg ggcccaaact ctatcaacat
//
LOCUS       DASPUL010000415         3970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_345721, whole genome shotgun sequence.
ACCESSION   DASPUL010000415 DASPUL010000000
VERSION     DASPUL010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345721"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VEJ00_10045"
     CDS             <1..1479
                     /locus_tag="VEJ00_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01544.1"
                     /translation="AARMLPSTRGSALRPKGTTMTRQFSLVYQTRFFVTPFLFALTAP
                     SFAQAAKPSNPAPCRVEAVDYKGWQAQQLSNRWLQLVVVPQNGGRLMQVTFAGHSYLF
                     VNPKLAGKYLPPASEHWFNYGGDKLWLLPEGNDDEQHWPGNSDLLDDGPYDFRKLSEG
                     QHCEIELTSPADPQTGVQFQRTISLDADSPRIRFHATMKNISGHSLEWSMQSVSQYDT
                     GNAIAFGSGPSDSSSASRFNHDFWTFTPANPQSSYLNRYHVRFGPAENPAVAVRDDGL
                     FTVHYVHMAAELWLDSTDGWLAVVDGSSHYSMVERFQYEQSKPYPGRASVIFWTNGPE
                     MRLSSDGIPSLSADSDAGPYYLEAELNSPMCRLRAGESCSFETEWFPTRAGSEFHGVT
                     DAGTIIQPLRATRLDSGKIKLVASFGVFFSGRMVARLYNEHGGLFGTMPVGDVTPAEP
                     VLLDTEITPPGKPARLSLHLEDEQGVDRGTLAEVKINSAESR"
     gene            1479..3119
                     /locus_tag="VEJ00_10050"
     CDS             1479..3119
                     /locus_tag="VEJ00_10050"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXX01545.1"
                     /translation="MPPVIKACSLILAGVLALTAFPRAREHGQSPITATPNVTNDDLL
                     AQPVGAYWTSYNGDYSGRRYSSLQEITVANVAKMQAAWVFHPGNSQRLEATPVVVRGI
                     MYVTSANDAFALDARTGRVLWHHSRPESSGLLDDAAGHKNRGVAVWQNWVYMETDDAH
                     LLCLDARSGSLRWDVEYADKTRHYGATSAPLVVKDTVIVGTSGGDSGVRGFVAAYDAA
                     TGSLKWRLWTIPGPGEFGSASWPGDAYLHGGGTTWMPGTYDPDLNILYWTTSNPAPDF
                     VGDTRPGDDLYTASVLAIDPDTGKMKWYFQFTPHDLYDYDGNETPVLVDVQENGATQH
                     LLLQANRNGFFYVLDRTNGKFLHATPFVEKLNWAKAIDASGRPVLTGRIPTADGTYIC
                     PGIDGATNWFSPSYNPKTGLFYVIALEACNLYFSDPKPFVRGQTYYDTGTKLPPDEHA
                     QKILLALSLDGKTVWRYPQVGSGDSWGGTLTTGGGLVFFGDDSGSLEAVDAATGRALW
                     HFNTGQGMRASPMTYAVDGIQYVAIAAGSNVFSFALPH"
     gene            complement(3142..>3970)
                     /locus_tag="VEJ00_10055"
     CDS             complement(3142..>3970)
                     /locus_tag="VEJ00_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01546.1"
                     /translation="FLPILALVCLACPHAWGQAQGATPQASGSASTQIPVDQENSRQA
                     KAIIERGIQSLGGPSYLNIRDREAQGRTYSLHHGQQTSNGVLFWSFSEFPDKERVELT
                     KERDVTELYVGNKGYEITYKGVHPIEEKDLVDYMRRRRYSLDVVLRTWVNDPGVILLY
                     EGNATAAQHPAQQVTLINSKDESVTLYFDVDTHLPVKKSFTWRDPIDKQKNLEEEVYD
                     NYRQVSGVMVPFNTTRYFNGDMAAERFLFSASMNQGLDQAMFDPNSGYNPNKQSGKH"
BASE COUNT          772 a         1324 c         1082 g          792 t
ORIGIN      
        1 gcggcacgca tgctaccctc gacccgcggc tcagctcttc gaccaaaagg cacaactatg
       61 actcggcaat tctctctggt ttaccagacc cgattcttcg ttacaccctt tctcttcgcc
      121 ttgaccgcgc cgagttttgc ccaagccgcg aagccatcga accccgcccc ctgccgcgtc
      181 gaagccgtcg attacaaagg atggcaggcg caacagctct cgaaccgctg gctgcagctc
      241 gtggtggtcc cccagaatgg cggacggctc atgcaggtca cttttgccgg acattcttac
      301 ctcttcgtca atcccaagct cgcgggcaaa tacctgccgc cggcttccga acattggttc
      361 aactacggcg gcgacaagct ctggctcctg cccgagggca acgacgacga acagcactgg
      421 ccgggcaact ccgatctgct ggacgacggc ccttatgact ttcgaaagct ttcggaggga
      481 cagcactgtg agatcgagct caccagcccc gccgatcctc agactggagt gcagttccag
      541 cgcaccatta gccttgacgc cgattctccg cgcatccgct ttcacgccac catgaaaaac
      601 atcagcggac attcccttga atggtcgatg caatcggtgt cgcagtacga cacgggcaac
      661 gcaattgcgt ttggctcggg cccgtccgac tcgagcagtg cctcgcgctt taaccacgac
      721 ttctggacct tcactcccgc caatccgcag agcagctatc tcaaccgtta tcacgtgcgc
      781 ttcggtcccg ccgaaaatcc cgcagtcgca gtgcgagacg atggtttgtt cacggtccac
      841 tacgtccaca tggccgcaga actgtggctg gactcgaccg acggctggct ggccgtcgtc
      901 gacggcagca gccactattc catggtcgag cgctttcagt acgaacagag caagccctat
      961 cccgggagag cctccgtcat tttctggacc aacggccccg aaatgcggtt gagcagtgac
     1021 ggcatcccat ccctgagcgc ggattcggac gccggcccct actatctcga agcggagctc
     1081 aatagcccca tgtgccggct tcgcgccgga gaaagctgca gctttgaaac cgaatggttt
     1141 cccacgcgag cgggaagcga gttccatggc gtaaccgacg ccggcacgat cattcagcct
     1201 ctgcgcgcga ctcgtctcga cagcggcaaa atcaagctgg tcgcctcttt cggcgtcttt
     1261 ttctccggcc gcatggtcgc ccgcctctac aacgaacacg gcggactctt cggaaccatg
     1321 cccgtaggag atgtgactcc tgccgagccg gtcttgctcg acactgaaat cacccctccc
     1381 ggaaaacctg ctcggctctc cctccatctg gaggacgagc aaggcgtaga tcgcggcact
     1441 ctcgcggaag tgaagatcaa ttcggcggag agccgctgat gccgcccgtt atcaaggcct
     1501 gctcgctgat cctggcggga gtcttagccc ttaccgcatt cccccgagca cgagagcacg
     1561 ggcaatctcc aatcaccgcc actcccaatg tcaccaacga tgacctcctg gctcagcccg
     1621 tcggcgcgta ctggacctcc tacaacggtg actacagcgg ccgccgctac agcagcctgc
     1681 aggaaatcac ggttgccaac gtcgcgaaga tgcaggctgc gtgggtcttc caccccggca
     1741 actcgcagcg gctcgaagcc acgcctgtgg ttgtgcgcgg cattatgtac gtcacctccg
     1801 ccaacgacgc tttcgccctc gatgcgcgaa ccggccgggt cctgtggcat cactcccgtc
     1861 ccgaaagctc gggcctgctc gacgatgccg ccggtcacaa aaaccgcggc gtcgccgtct
     1921 ggcaaaactg ggtttacatg gaaaccgacg acgcccactt gctctgcctc gacgcccgct
     1981 ccggcagcct gcgctgggat gtcgaatacg ccgacaagac caggcactac ggcgcgacca
     2041 gcgccccgtt ggtcgtgaaa gataccgtga tcgtcggcac ctccgggggc gactccggcg
     2101 tgcgcggatt cgtggccgct tacgatgccg ccaccggaag cctgaagtgg cgcctgtgga
     2161 ccatcccggg cccgggagaa ttcggctccg cgagctggcc cggcgatgct tatctccacg
     2221 gtggcggcac gacctggatg cccggaactt atgatcccga cctcaacatt ctctactgga
     2281 ccacgagcaa cccagccccg gacttcgttg gtgacacccg ccccggcgac gatctctaca
     2341 ccgcctcagt ccttgccatc gaccccgaca ccggcaaaat gaaatggtat ttccagttca
     2401 ctccgcacga tctctacgac tacgacggca atgaaactcc ggtgctggtc gacgtgcagg
     2461 aaaacggcgc gactcagcat cttctgctgc aggcgaaccg caacggcttc ttctatgttc
     2521 tcgaccggac caacggcaaa tttctccacg ccacgccgtt cgtcgaaaaa ttgaactggg
     2581 cgaaggccat cgacgcctcc ggtcgccccg tcctcaccgg ccgaatcccg actgcagacg
     2641 gcacctatat ctgccccggc atcgacggcg ccaccaactg gttctctcct tcctacaatc
     2701 cgaagactgg cctcttctat gtcatcgcgc tggaagcctg taacttgtat ttctctgacc
     2761 caaaaccgtt cgtccgcggg cagacttact acgacaccgg cacgaagctt ccacctgatg
     2821 agcatgcaca gaaaatcctg ctggctctct ctctcgacgg gaaaacggtc tggcgctatc
     2881 cccaggtggg ctcgggcgat tcgtggggcg gcacgctcac aaccggcggt gggttggtct
     2941 tcttcggaga tgattccggc tcactcgaag cggtcgacgc ggccaccggc cgcgctctct
     3001 ggcacttcaa caccggccag ggcatgcgcg cttcgccgat gacctatgcg gttgacggaa
     3061 tccagtacgt cgcaatcgcg gcgggcagca acgtgttctc tttcgcgctc ccgcactgat
     3121 cccgctaacc gcggcattgc ctcagtgctt gccggactgc ttgttcgggt tgtagcccga
     3181 gtttggatcg aacatcgcct gatcgagacc ctggttcatg ctcgccgaga aaagaaatcg
     3241 ctcggcagcc atgtcgccat tgaagtaacg agtcgtattg aagggaacca ttacccctga
     3301 aacctggcgg tagttgtcgt aaacctcttc ttcgagattt ttctgcttgt cgatgggatc
     3361 gcgccaggtg aacgacttct tgaccggcaa gtgcgtgtcg acgtcgaaat acaacgtgac
     3421 gctttcgtct tttgaattga tcaatgtcac ctgttgcgcc gggtgctgcg ccgctgtggc
     3481 attgccttca tagagcagga taactccggg atcgttcacc caggttcgga gcacgacatc
     3541 gagcgaatac cgccggcgtc gcatgtagtc gaccagatcc ttttcttcga tcggatgaac
     3601 tcccttgtag gtgatctcat accccttgtt gccgacatac agttcggtga cgtcgcgctc
     3661 tttcgtgagt tccacccgct ccttgtcggg aaactcgctg aaactccaga acagaacgcc
     3721 gttgctggtc tgctgcccgt gatggaggct gtatgtccgc ccctgcgcct cgcgatcgcg
     3781 aatgttgagg taacttggac cgcccagcga ctggattccc cgctcgatga tggccttggc
     3841 ctggcgagag ttctcctgat cgaccggaat ctgagtcgag gccgagcccg aggcttgagg
     3901 cgtggcaccc tgcgcttgtc cccacgcatg ggggcaagcc aggcacacca aagccaggat
     3961 aggaagaaac
//
LOCUS       DASPUL010000416         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1298483, whole genome shotgun sequence.
ACCESSION   DASPUL010000416 DASPUL010000000
VERSION     DASPUL010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298483"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VEJ00_10060"
     CDS             complement(<1..1025)
                     /locus_tag="VEJ00_10060"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HXX01547.1"
                     /translation="MPESIEQILASYNDRAPLSEASTIPGSWYVDPRMAELERLNVFS
                     KTWQLVARTDQVQKPGQFVATRLAGEPVVVVRDSDGMLRAFYNVCRHHAAAVVTQPCG
                     QAAILQCPYHGWKYGLDGTLKGMPEFEGVANFDRAENGLVPIRVETWECFVFVNLDPQ
                     AAPLKEFLGGLVKRVAPLGISTLHYFDRRTYDIRCNWKVFVDNYLDGGYHVPHLHKGL
                     SSVLDYKAYTIENEDRYCLQSSPMVASDEDAATGATRKGDRAWYFWQYPNLMINCYQG
                     YMDTNYVIPVDADHCTVIFDFYFSDVSEARCEYNTQSVNVGNRVQDEDLGICEDVQRG
                     LKSRAYGA"
     gene            complement(1103..1828)
                     /locus_tag="VEJ00_10065"
     CDS             complement(1103..1828)
                     /locus_tag="VEJ00_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXX01548.1"
                     /translation="MPGIASNRVAPPQYQRQVPFRPRSEAKRARIIEAATLHFAEHGY
                     HAARVGDIASALGIAKGSIFQHFGSKDGLFFEVYKRAVRSFAKYLDAPADIRAQGFFE
                     VLRYWLVRTEHMLHEDWIPYRVSLLGNYGTDLVLKREINRFLVTEDPYGTVSFVRFGL
                     ERRELRRDMDIEMIVSILDWMMERFQDALLTAELDPGLFRRQGELAEKKEARIQQFLA
                     VLQRAIGASPNHGVGPSGAAHHR"
     gene            1843..>2386
                     /locus_tag="VEJ00_10070"
     CDS             1843..>2386
                     /locus_tag="VEJ00_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXX01549.1"
                     /translation="MKQYTRSTFCFNFIFTAECDTVIRFELDSVTSGQQTTANSPSRY
                     DAIIVGAGHNGLTAAAYLARAGLSTLVLERRDIVGGCCVTEEIAPGCRVSTTSYIASM
                     LRPEVISELRLADHGLRMIPCDPAIQVPFPDGHVVPWWVDRERARQEFSRISAKDAAR
                     FVQVDDQLKKLARYLQPFFME"
BASE COUNT          498 a          698 c          684 g          506 t
ORIGIN      
        1 gcgccgtaag cgcgggactt gaggccgcgc tgcacgtctt cacaaatccc aagatcctcg
       61 tcctgcacgc ggtttcccac attgaccgat tgtgtgttgt actcacaccg cgcctcgctc
      121 acgtcgctga agtaaaaatc gaaaatcact gtgcagtgat cggcgtcgac ggggatcaca
      181 tagtttgtat ccatgtatcc ctggtaacaa ttgatcatca ggttcgggta ctgccagaaa
      241 taccaggcgc ggtcaccctt gcgcgtggcg ccggtagcgg cgtcttcgtc ggaagcgacc
      301 atgggactcg attgcaggca gtagcggtct tcgttctcga tcgtgtacgc cttgtagtcg
      361 agcacagaac tcaaaccctt gtgcaggtga ggcacgtggt aaccgccgtc gaggtagttg
      421 tcgacaaaaa ctttccagtt gcagcggatg tcataggttc tgcggtcaaa gtaatgcagc
      481 gtgctgatgc cgagcggggc aacccgcttg accagtccgc cgaggaattc cttgagtggc
      541 gcggcctggg ggtcgaggtt cacgaagacg aagcactccc aggtttcgac gcgaatcggg
      601 actaaaccat tctccgcccg atcgaaatta gcaacgcctt cgaattcggg catacctttc
      661 agcgttccgt cgagcccata cttccagccg tgatacggac attgcagaat cgcggcctgg
      721 ccgcacggtt gcgtgaccac tgccgcggcg tgatggcggc agacattgta gaaggcgcga
      781 agcattccat cgctgtcccg aacgacgacc acgggctctc cggccaaccg cgtcgccacg
      841 aactgtcccg gcttctgcac ctgatcggta cgggcaacga gttgccacgt cttgctgaag
      901 acgttgaggc gctcgagttc cgccattcgc gggtcgacat accaagaacc agggatggtc
      961 gaggcctccg acagtggcgc gcgatcgttg tagctggcaa gaatctgttc gatggattct
     1021 ggcatcgtta tgctctcaag ttcttactta gaaagcggtc gatctgatct cgcgctgagc
     1081 gagaagcagc gcattgcact cctcaacggt gatgcgcggc cccggacggc ccgacaccgt
     1141 gattcggaga cgcgccgatg gcccgttgta agaccgcgag aaactgctga attctggctt
     1201 ctttcttctc ggccagttct ccctggcggc ggaacaatcc ggggtcgagc tcagcagtca
     1261 gcagcgcatc ctgaaaccgc tccatcatcc agtcgagaat ggaaacgatc atctcgatgt
     1321 ccatgtcgcg gcgcaactcg cggcgctcca gaccgaagcg tacgaaactc acggttccgt
     1381 atggatcttc ggtgacgagg aagcgattta tctcgcgctt cagcacaaga tcagtgccgt
     1441 aattgcccag cagcgagacg cgatacggaa tccagtcttc atgcaacatg tgttccgtgc
     1501 gcaccagcca gtagcgcagc acttcgaaaa agccttgcgc acgaatgtcg gccggcgcat
     1561 cgagatattt cgcgaacgaa cgcaccgccc gtttgtagac ctcgaaaaat aacccatctt
     1621 tgctgccgaa atgctggaag atcgacccct tggcaatgcc taaggcggag gcgatgtcgc
     1681 cgacccgggc cgcgtgataa ccgtgctcgg cgaaatgcag cgtggccgcc tcgataattc
     1741 gggcacgctt ggcctccgac cttggacgga atggcacctg gcgctggtac tgcggcgggg
     1801 caactcgatt gctggcgatt ccgggcataa tgactctaag tcatgaaaca atataccagg
     1861 tcgactttct gcttcaactt catttttacg gctgagtgcg atactgtgat tcgattcgaa
     1921 ttggacagcg tgacttcagg tcaacagaca acggcgaatt ccccatcccg ctacgacgcc
     1981 attatagtcg gcgccggcca caacggactg acggctgctg cttatctcgc acgtgccggt
     2041 ctgtcgacac tggtgcttga gcgtcgcgat atcgttggcg gatgctgcgt gacagaagag
     2101 atcgcgcccg gatgccgtgt ctctacgacc tcctacatcg ccagcatgct gcggccggag
     2161 gtgatttccg agctgcggct ggctgatcat ggcctgcgga tgatcccttg cgatccagcg
     2221 atccaggttc ccttccctga cggccacgtc gtcccgtggt gggttgaccg cgagcgggca
     2281 cggcaggaat tttcccggat ttccgcaaaa gatgctgcac gatttgtgca agtcgacgat
     2341 cagctgaaaa aactggcacg ctacttgcag ccatttttca tggaac
//
LOCUS       DASPUL010000417         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1171567, whole genome shotgun sequence.
ACCESSION   DASPUL010000417 DASPUL010000000
VERSION     DASPUL010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171567"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /gene="proB"
                     /locus_tag="VEJ00_10075"
     CDS             <1..675
                     /gene="proB"
                     /locus_tag="VEJ00_10075"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HXX01550.1"
                     /translation="VDQFDLSKNEYLIVTSGAIAAGMSGLGLRERPKDVRGMQACAAV
                     GQSKLMHTYERLFFGKKVVAQLLLSSDDFTSPVRYRNLQNTLAELLSLGVVPIINEND
                     SVSVRELEGAFGDNDELSSLLATAVQADWLLLLTNVDGFLLPNGRRKPRLQRVIHKLT
                     PALEAQCNGKSPLGRGGMVSKLRAAKLASKAGVRVAILNGRHPQAIKLGMERKIGTYF
                     PPQKGR"
     gene            683..1933
                     /locus_tag="VEJ00_10080"
     CDS             683..1933
                     /locus_tag="VEJ00_10080"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HXX01551.1"
                     /translation="MPTVTTAASTHEKLLRARVAAARLGLLSTQQKNALLLAIANAIE
                     ADAGSILAANCEDLETSGRDGAMRDRLLLTPERIRDMARGVREVAALPDPVGETLAEW
                     TRPNGLRIRKVRVPLGVIGIIYESRPNVTVDTAVLALKAGNAIVLRGGKEAARSSQRL
                     VDIMAAVPGLPEGAIELLDSSSRQSVQELIKARGLVDVIIPRGGAELISYVAENSVVP
                     VIETGAGNCHIFVDESADFDMADRIVLNAKTQRPSVCNAGEKLLVHERIAAGYIPRIV
                     KKLIDVGVEVRGDGKARTLAAGLLVKPAAEQDWHEEYLRLCMAVAVVSDVDDAIAHVN
                     RYSTKHSEAIITRNETNARKFLRGVDSAAVYWNASTRFTDGAEFGFGAEMGISTQKLH
                     CRGPFALAELTSSKYEVVGTGQVR"
     gene            2128..>2484
                     /locus_tag="VEJ00_10085"
     CDS             2128..>2484
                     /locus_tag="VEJ00_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01552.1"
                     /translation="MKLFSAAVLFSFTLLPSSSTSSGSPPPTSPIFGFRDAAAESAVE
                     TRFVAIPDAKLAEEHLRTITKAPHMAGTPEDKATADYVAQKFREAGLDTEIVEYKVWI
                     NYPSEISVDLTAPSGVE"
BASE COUNT          492 a          808 c          732 g          452 t
ORIGIN      
        1 gtcgatcagt tcgatctctc gaagaacgaa tacctgatcg tgacctcggg tgccatcgcg
       61 gccggaatgt cgggcctggg tctgcgcgag cgcccgaaag acgttcgcgg catgcaggcg
      121 tgcgcggccg tcggccagag caagctgatg cacacctacg agcgcctgtt cttcggcaag
      181 aaagtcgtcg cgcagctgct gctctctagc gatgacttta cttctcccgt gcgctatcgc
      241 aacctgcaga acacgctggc cgaactgctc tcactcggag tcgtgccgat catcaacgag
      301 aacgacagcg tcagcgtgcg cgaactcgaa ggcgccttcg gcgacaacga cgagttgagt
      361 tcgctgctgg ccaccgccgt gcaagccgac tggctgttgc tgctgaccaa cgtcgatgga
      421 ttcctgcttc ccaacggccg caggaaaccg cgcctgcagc gcgtcatcca caaacttacg
      481 ccggcgctcg aagcgcagtg caatggcaag agtccgctcg ggcgcggcgg catggtttca
      541 aagctgcgcg cggccaagct ggcaagcaaa gcgggagtgc gcgtcgcgat cctgaacggg
      601 cgccatcccc aggccatcaa gctgggcatg gaacgcaaga tcggaacgta ctttccgccg
      661 cagaagggca ggtaaaccgc aaatgcccac cgtgaccacc gccgcctcta cgcacgaaaa
      721 gctgttgcgc gcccgtgtgg ctgcagccag gctcggtctg ctttcaacgc agcaaaaaaa
      781 tgccctgctt ctcgcaatag ccaacgcgat cgaggccgat gcaggaagca tcctcgccgc
      841 caactgcgaa gacctggaga cttccggccg cgacggcgcc atgcgcgacc gcctgctgct
      901 gactccggag cgcatccggg acatggcgcg cggtgtgcgc gaagttgcgg ccttgcccga
      961 tcctgtcggc gaaacgctcg ccgaatggac gaggccaaac ggcttgcgca tccgcaaagt
     1021 gcgagtaccg ctcggagtga tcgggatcat ctacgagtcg cgcccgaatg tcacggtaga
     1081 cacagccgtg ctcgccctta aagccggcaa tgccatcgtg ctgcgcggcg gcaaggaagc
     1141 ggcgcgaagc agccagcgcc tggtcgacat catggccgca gtgcccggcc tgcccgaagg
     1201 agctatcgag ctgctcgact cgagctcgcg ccagtccgta caggagctga tcaaggcgcg
     1261 cggactcgtc gacgtgatca tccctcgcgg tggcgcggag ctgatctcct atgttgccga
     1321 gaactctgtc gtgcccgtca tcgaaaccgg ggcggggaac tgccacatct tcgtcgacga
     1381 atccgccgac ttcgacatgg ccgaccggat cgttctcaac gcaaaaacgc agcgcccgtc
     1441 ggtctgcaat gcgggagaga agttgctggt gcacgaacgc atcgcggcgg gttacattcc
     1501 ccggatcgtg aagaagctga tcgatgtcgg tgttgaagtg cgcggtgacg gcaaagcccg
     1561 caccctcgct gctggtttgc tggtaaagcc cgctgccgag caggactggc acgaggaata
     1621 tctgcgcctg tgtatggcgg tggccgtggt ctccgatgtg gacgacgcca tcgcgcacgt
     1681 caatcgctac tccacgaagc attccgaagc gatcatcacc cgcaacgaga ccaacgcgcg
     1741 caagttcttg cgcggcgtcg attccgcggc ggtgtattgg aatgcctcga cgcgcttcac
     1801 cgacggtgcc gaattcggtt tcggcgccga aatgggcatc agtacccaga aactccactg
     1861 ccgcggcccc ttcgccctgg cggagttgac ttcttcgaag tacgaggttg tggggacggg
     1921 ccaggtgcgg taggcttcgc ggccagtacc ttgcgggcat aggtgaactt cgccagatac
     1981 ttgtcgtgat tgatactcac gtcgctgcag ccgctctttc cgagcggtgt aagcttcaga
     2041 ggcttgaacg gtcgcttcac aatatctttc cgggcactac ctttaatcgg tcctgtgcta
     2101 ccttactacg ttcacgcgat gccccccatg aaacttttct cagctgccgt tcttttctct
     2161 ttcacgttgc tgccctcatc atccacaagt tccggaagcc cgcccccgac ctcacccatc
     2221 ttcggcttcc gcgacgccgc ggccgagtcg gcggtcgaaa cccgtttcgt cgccattccc
     2281 gacgccaaac ttgccgaaga acatttgcgc accatcacca aagccccgca tatggcaggc
     2341 acccccgaag acaaggccac agccgactac gtcgcccaaa aattccgcga ggccggccta
     2401 gacaccgaaa tcgtcgagta caaagtctgg atcaactatc cctccgaaat cagcgttgac
     2461 ctgaccgcgc cctccggtgt ggaa
//
LOCUS       DASPUL010000418         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_626609, whole genome shotgun sequence.
ACCESSION   DASPUL010000418 DASPUL010000000
VERSION     DASPUL010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626609"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VEJ00_10090"
     CDS             complement(<1..288)
                     /locus_tag="VEJ00_10090"
                     /inference="COORDINATES: protein motif:HMM:NF037081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXX01553.1"
                     /translation="MTKADLIDEVSRLAELTRKDSEVIVETIFDSVVRSLRAGDKIEI
                     RGFGSFRTRQRKPRVGRNPKTGERVEVPAKKIPFFKPSKELKDLVNETTPGA"
     gene            complement(580..1491)
                     /gene="sppA"
                     /locus_tag="VEJ00_10095"
     CDS             complement(580..1491)
                     /gene="sppA"
                     /locus_tag="VEJ00_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HXX01554.1"
                     /translation="MSDGRSRTLIWILIGGGAFFLFVLAVFSIVYLVLHAGGSEAAMP
                     SFGDRIGVVDLDGVIISPQPVVGQLKKFGDDSSIKAIILHVNSPGGGVAASEEIYREV
                     KRLRKEKNKKVVVSVETACASGAYYVASGSDKIYANNGSIVGSIGVIAQWTSYGDLLK
                     WAKLKDVVIKTGEFKDTGNPARDLTPAEQAYMQSLIDSMFGQFIQVVADGRGLKTEDV
                     RSIANGKVWTGEQAKSMKLIDSLGDFETVVSETAKSVGIRGEPTLVRPERERKTLMDL
                     LLGDISQYLPDRATMLDQHVGFYYLWR"
     gene            complement(1574..2476)
                     /locus_tag="VEJ00_10100"
     CDS             complement(1574..2476)
                     /locus_tag="VEJ00_10100"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXX01555.1"
                     /translation="MRAAAILGLGCSPRNLVPFQQDKGVEWRLGMPAAGDEVDVILLF
                     GGDGTIHRHLGQLVKLGLPVLVVPAGSGNDFARALGLRRIQDSNAAWRRFRESRVNVR
                     TIDLGVISPPTKEEDLGDKSLHDSQPGMRYFCCVAGVGLDCEVARRANQLPRWLRGHG
                     GYVLSLAPTILGFAPFPMKILVAEQGRDWSVRSDQPTILAAFANAPVYGGGMKIAPLA
                     QMDDGLLDVCVVGGVNAFKLFCLFPTVYSGRHLKIREVKYFQAGRVRIETEHPLDVYA
                     DGEFVCQTPVEISVARSALKVVTL"
     gene            2661..>3638
                     /locus_tag="VEJ00_10105"
     CDS             2661..>3638
                     /locus_tag="VEJ00_10105"
                     /inference="COORDINATES: protein motif:HMM:NF025264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HXX01556.1"
                     /translation="MRLRPSTLALCFLALSFGLSGCGFSNGPKNTGGGDTTAPSITTQ
                     PANQTVTVGKTATFKVVATGTGLSYQWQKGTTAITGATSASYTTPATKLTDSGSKFSV
                     VVSNSAGNVTSNAATLTVDSAPVAPSITQQPSNQTVTVGQTATFSVKATGTTPLTYQW
                     QKGTTPISGATSATYTTPATTSADNGSQFKVVVSNSVGKVTSNTATLTVNTPPPPPPG
                     TTDVLTYHNDIARTGQNLTETALTTSTVNSSKFGKIGFYSTDGLVDAEPLIASQVAVP
                     GNGTHDLLIVASEHGTVYAFDANSGATIWKKSTLLSGETTSDDRGCGQVS"
BASE COUNT          779 a         1162 c         1017 g          680 t
ORIGIN      
        1 tgccccggga gtggtctcgt tcactaggtc tttcagctct ttgctgggct tgaagaacgg
       61 gatcttcttt gccggcactt ccacgcgctc gccggtcttc gggttgcggc cgacacgggg
      121 cttgcgctgg cgtgtgcgga agctgccaaa gccgcgaatc tcgatcttgt ccccagcgcg
      181 cagcgagcgc accacgctgt caaagatcgt ttccacgatc acttcgctgt ctttgcgcgt
      241 aagttccgcc aaacgcgaga cttcgtcaat cagatcggct ttggtcataa cccctcttcg
      301 ttcccgagag aaccctattg aaatctaacc ccggctgctg gcagggtccc tgccaaggca
      361 ccggaacctc cataaccttc tcaaatttca ggggttgtgt gcaagtgcct agcgaggatg
      421 caggcttttg cgggtctgta gcgccaagag aaccgaatga gaagctcgtt ccaggaagag
      481 aattgtcacg gcgtgcggga aatccaccgt tgatgcgtag acagacccag cacaaggagg
      541 tgaccgcaat ttccctggtg caggcgttcc tgtcggcagc tacctccaca gatagtaaaa
      601 gcctacgtgc tggtcgagca tggtcgcgcg gtcgggcagg tactgtgaaa tatctccgag
      661 cagaaggtcc atcagtgtct tgcgctcccg ctccggacgc accagggtcg gctcgcctcg
      721 aatccccacg gatttcgcgg tctccgagac tacagtttcg aagtcgccca ggctgtcgat
      781 gagcttcatg gacttggcct gctcgccggt ccagactttg ccgttggcga tcgaccggac
      841 gtcctcggtc ttcaggccgc gcccatcggc cacaacctga atgaactggc cgaacatgct
      901 gtcgatcagc gactgcatgt aggcctgctc ggccggggtg aggtcacgcg caggatttcc
      961 tgtgtctttg aactcgccag tcttgatgac gacatctttc agcttcgccc atttcagcag
     1021 atcgccgtag ctcgtccact gcgcgatcac accaatggag cccacgatgc tgccgttatt
     1081 ggcatagatc ttgtcggaac cggaggctac gtaataggcg ccgctggcgc aggcggtttc
     1141 gaccgaaaca acgactttct tgttcttctc tttgcgcagc cgcttcactt cgcgatagat
     1201 ctcttccgac gccgccacgc ccccgcccgg cgagttcacg tgcaaaatga tggccttgat
     1261 cgaagagtcg tcgccgaatt tcttcaactg cccgacgacg ggctgcggag aaatgatgac
     1321 accatcgaga tcgacgacgc cgatacggtc gccgaagctg ggcatggccg cttcacttcc
     1381 gccggcgtgc agcaccagat agacgatcga aaacacggcc agcacgaaga ggaaaaaagc
     1441 tcctccgccg ataagaatcc agatcagggt gcgtgaacgt ccgtcagaca tggagagaag
     1501 tttagcacca gtctggatgg gacatggctg gtttcatgac gacggacctt cccatcagaa
     1561 agctatgcgt acctcacaag gtcacaacct tcaaggccga acgtgcgaca gaaatctcta
     1621 ccggcgtctg gcagacaaac tccccgtccg cataaacatc cagtggatgt tcagtctcga
     1681 tccgcacccg tcccgcctga aaatacttca cttcgcgaat tttcagatgc cgtccgctgt
     1741 ataccgtggg gaacaagcaa aacagcttga atgcattcac gccgcccacg acacacacat
     1801 cgagcaggcc gtcgtccatc tgggccaggg gtgcgatctt catcccgccg ccatacaccg
     1861 gagcattggc aaacgccgcc agaatcgtgg gctgatcgct gcgcacgctc caatcacggc
     1921 cctgttccgc gacaagaatc ttcatgggaa acggcgcaaa gccaagaatt gtcggcgcca
     1981 gactgagcac atagcctccg tgtccgcgca gccagcgtgg cagctgattg gcgcggcgcg
     2041 ccacttcgca atccagaccg acacccgcca cacaacagaa gtagcgcatg ccgggctgcg
     2101 agtcgtgaag agatttgtcg ccgaggtcct cttcctttgt cggaggtgag atcacgccga
     2161 ggtcgatggt gcggacgttg actcggctct cgcggaagcg tcgccacgcc gcattcgaat
     2221 cttgaatccg ccgcagcccg agcgcccggg caaaatcgtt cccgctgccg gcaggcacca
     2281 ccagcaccgg gagtccgagc ttgacaagct gcccaagatg ccggtgaatt gttccgtcgc
     2341 cgccgaagag caggatcaca tcgacctcat cgcccgcggc aggcatcccg aggcgccatt
     2401 cgacaccctt gtcttgctga aacggcacca gattcctggg cgagcatccc aggccgagaa
     2461 tggcagcggc gcgcattgct agcgcgactg agagtaccag agtcgaagcc gatcccagcc
     2521 agcaatcggc cgtgcggcga gataaggcag gatgagaata cggcccggcg gacggccccg
     2581 gacatgtccg atcgtaatgc cgggaaatcc ccggacggct gtcatgatgg tcgcaaagtt
     2641 cctttacggg gatcccactt atgcggttgc gaccttcgac gctcgccctg tgctttcttg
     2701 cacttagctt tggtctgtcc ggatgcggct tcagcaacgg gcccaagaac acgggcggag
     2761 gcgacacaac cgccccgtcg attacgacgc agcctgcgaa ccagacggtt acagtcggta
     2821 agacggcaac cttcaaggtg gtcgccaccg gcacgggctt gagctatcag tggcagaagg
     2881 ggacgactgc gatcaccggc gcaacctcgg cgagttacac gactcccgcg accaagctga
     2941 ccgacagcgg ttcgaagttc tcggtggtgg tcagcaactc ggcggggaac gtcaccagca
     3001 acgcagccac actgacggtg gactctgcgc ccgtcgcacc ttccatcaca cagcagccat
     3061 cgaaccagac cgtcaccgtc ggtcagacgg caactttctc agtcaaggca acggggacca
     3121 ccccgctaac ctaccagtgg cagaaaggta ctaccccgat cagcggtgcc acctcggcca
     3181 cgtatacgac cccggcgaca acctccgctg acaacggatc acaattcaaa gtggtcgtga
     3241 gcaattccgt ggggaaggtc accagcaata cagccacact cacggtgaat actccaccgc
     3301 ctccgccccc aggcactacc gacgttctga cttatcacaa tgacattgcg cgtaccggac
     3361 agaatctgac cgaaacggcc ctgaccacaa gcaccgtgaa ctcgtcgaag ttcggcaaga
     3421 tcggcttcta ctccaccgat ggcctggtcg acgccgaacc gctcatcgcc tcgcaagtcg
     3481 ccgttcccgg caacggcacg catgacctgc tcattgtggc ctccgagcac ggcacggttt
     3541 acgcctttga cgccaacagc ggcgccacga tctggaagaa gtcgacgctg ctctcgggtg
     3601 aaacaacctc cgacgatcgc ggttgcggcc aggtcagc
//
LOCUS       DASPUL010000419         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1996917, whole genome shotgun sequence.
ACCESSION   DASPUL010000419 DASPUL010000000
VERSION     DASPUL010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1996917"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(434..2464)
                     /locus_tag="VEJ00_10110"
     CDS             complement(434..2464)
                     /locus_tag="VEJ00_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HXX01557.1"
                     /translation="MIQLSGAGKRFGHKLLFENVDWLITANDHIGLVGANGTGKSTLM
                     KILAGMDTLDYGALTVAKGTSAGYLPQDGLTLSGRTVFAECMSVFDDLRAMEQELESL
                     MHSMAELDHTSAEYDAVAERYTRVEHEFRTRDGYSIEAEVGRVLMGLGFTKEDWQRQT
                     DEFSGGWQMRLALAKLLLQKPNLLLLDEPTNHLDLEARNWLEEYLQDYPHAFVLISHD
                     RYFLDVTVDKIAEIWNKRFWFYTGNYDKFLAQKTQRNEQLQAAYRNQRERIDQLEVFI
                     NRFRYQATKAKQVQSRIKELERMERIEVPPEEKTIHFKFPQPKSSGRIVAEFEGVAKR
                     YPGRNGNGPKQVFDKVSFMIERGDRIALVGVNGAGKSTLIKLLAGDEPLSAGEYHLGH
                     NVQSDYFAQDQYKELDPDARILDDLGAISPRSTETELRSLLGCFLFSEDDVFKKIGVL
                     SGGERGRYALLRLLLHPANFLLLDEPTNHLDMRAKDVLLNALMDYTGTVVFVSHDRYF
                     IDKLATRVFEIGGGKVEIFPGNYEDYLWRKEGGPQMSPTLDDVPDVQPKKQEVTKNAG
                     APLKPAVGLSGGFDEVPTDGHGSANGDQSDTPKKRVNPIKRKQLQDRVHELEEEISRA
                     ETAIANLEAALQKFISADESQRQSQELGHYKASHAALIEEWEGLAASLQESE"
     gene            2601..>2848
                     /locus_tag="VEJ00_10115"
     CDS             2601..>2848
                     /locus_tag="VEJ00_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01558.1"
                     /translation="MLRVISAFCLLLTTTSSVRLAAQVYPYRDGTPGVTGYRSEVMAE
                     VMIQESEFVRLAEAIPADKYTWRPSLDVRTIAEVFLHA"
BASE COUNT          502 a          801 c          904 g          641 t
ORIGIN      
        1 ggaagcggct ggtctgtgaa atttgtggcc ttcggcagtt gctggccact tggccaagtt
       61 gaatatcgca cagaggtaag gggagagtga gattacggaa gatggtgtac gggcggagag
      121 gagggtttat taccagcgga cgagccgccg agctcgtgct ttgtgagact gcgcggatgt
      181 cccggaggga catttgaaag tagcccggcg ttttaacgcc gggtggcgag gggaaagggg
      241 ttgcgtcccg gagggacacc tgaaatccag cttactcgca gctgctcagc cgtgcctccg
      301 gcgcgggacg caaaatcgct cgccgctgcc cgccagtgaa ccggcgggct actgtcattg
      361 gtccctccgg gaccgacctt tctttcagga ttaacggtgt agtgtcggag gaatctactc
      421 cgccacctga tcttcactca gattcctgaa gactggctgc gagaccttcc cattcctcga
      481 tcaacgcagc gtggctggcc ttgtagtggc ccaattcctg cgactggcgc tgactctcat
      541 cggcactgat gaacttctgc agggcggctt cgaggttggc gattgcagtc tcagcgcggc
      601 tgatttcttc ttccagttcg tggacgcggt cttgcagttg tttgcgcttg atggggttca
      661 cacgcttctt gggagtgtcg gattgatcgc cattggcgga tccgtggcca tctgtcggga
      721 cttcgtcaaa acccccactc aagccaacag cgggcttgag tggggcaccc gcgttttttg
      781 taacttcctg cttctttggc tgtacgtccg gaacgtcatc gagcgtggga ctcatctggg
      841 ggccgccttc cttgcgccag agatagtctt cgtagttgcc ggggaagatc tcgaccttgc
      901 cgccgccgat ttcgaagacg cgcgtggcca gcttgtcgat gaaatagcgg tcgtgcgaga
      961 cgaagacgac cgtgccggta tagtccatga gcgcgttgag aagcacgtct ttggcgcgca
     1021 tgtcgaggtg gttggtgggc tcgtcgagca gcaggaagtt cgccggatgc agcagcaggc
     1081 gcagcagggc atagcgtccg cgctcgccgc cggagaggac gccaatcttc ttgaagacgt
     1141 cgtcttcgga aaagagaaag cagccgagca ggctgcgcag ttcggtctcg gtcgagcgcg
     1201 gcgagatcgc cccgaggtcg tcgaggatgc gcgcgtcggg atcgagttct ttgtactgat
     1261 cctgtgcgaa gtagtcagac tgcacgttgt ggccaaggtg gtactcgccc gcactgagcg
     1321 gctcatcgcc ggcgagcagc ttgatcagtg tcgatttccc cgcgccgttc acgccgacga
     1381 gcgcgatgcg gtcgccgcgc tcgatcatga agctgacttt gtcaaaaacc tgcttggggc
     1441 cgttgccgtt tctgcccggg tatcttttcg ccacgccctc gaactcggcc acgatccgtc
     1501 cgctggactt gggctgcggg aacttgaagt ggatggtctt ttcttccggc gggacttcga
     1561 tccgctccat gcgctccagt tccttgatgc ggctctgcac ctgcttggcc ttcgtcgcct
     1621 ggtagcgaaa gcggttgatg aagacttcga gctggtcaat gcgttctcgc tggttgcggt
     1681 aagcggcttg cagctgctcg ttgcgctgcg tcttctgggc gagaaacttg tcgtagttcc
     1741 cggtgtaaaa ccagaagcgc ttgttccaga tttcggcgat cttgtcgacg gtgacgtcga
     1801 gaaaatagcg gtcgtgcgag atcagcacga aggcgtgcgg gtagtcctgc agatattctt
     1861 ccagccagtt acgggcttca aggtcgaggt ggttcgtggg ctcatcgagc agcagcaggt
     1921 tcggcttctg cagcagaagt ttcgccagcg ccaggcgcat ctgccagccg ccggaaaatt
     1981 cgtcggtctg gcgctgccag tcttctttgg tgaaacccaa ccccatcagc accctgccga
     2041 cttcggcttc gatcgagtag ccgtcgcggg tgcggaattc gtgctcgact cgggtgtagc
     2101 gctcggcgac ggcgtcgtat tcggcgctgg tgtggtcgag ttcagccatc gagtgcatga
     2161 gcgattcgag ttcctgctcc atggcacgca ggtcgtcgaa caccgacatg cactcggcga
     2221 aaacggtgcg gccggagagc gtgagaccgt cctgcgggag atatccggcg gaagtgcctt
     2281 tggcgacggt gagcgcgccg taatcgagcg tgtccatgcc agcgagaatc ttcatcagcg
     2341 tggacttgcc ggtgccgttg gcgccgacca ggccgatgtg gtcgttggcc gtgatcagcc
     2401 agtcgacgtt ttcgaacagg agcttgtggc cgaagcgttt tccggcaccg gaaagctgaa
     2461 tcatgggctg gaattatcgt ctcataaacg ccgggccggc aggaccgata tcgttgcgcc
     2521 aaggagacga attctcccct gcggcctctg ggtataatct tctcgattca gcccattatc
     2581 cccgcaaaat ggaggccttc atgctacgcg tgatctccgc tttctgcctg ctgctgacaa
     2641 caaccagctc ggttcgtctc gcggcgcagg tgtatccgta ccgcgacggt actcccggcg
     2701 tcaccggata ccgctcggaa gtgatggccg aagtcatgat tcaggaaagc gaatttgtgc
     2761 gtctggccga agccatccct gccgacaaat acacatggcg tccttctctc gatgtccgga
     2821 ccatcgccga agtttttctg cacgccag
//
LOCUS       DASPUL010000420         4622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_485364, whole genome shotgun sequence.
ACCESSION   DASPUL010000420 DASPUL010000000
VERSION     DASPUL010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485364"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VEJ00_10120"
     CDS             <1..224
                     /locus_tag="VEJ00_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01559.1"
                     /translation="NPPQFRNVHADPSDATRAFLDLKARWMVPMHYGTFRLSHEPVDE
                     PLQLLDREARAAGIQDRVVVMEEGVTRFF"
     gene            404..892
                     /locus_tag="VEJ00_10125"
     CDS             404..892
                     /locus_tag="VEJ00_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXX01560.1"
                     /translation="MPVKPIPDGYHTLTPFLTVRDAARAIEFYKQAFGAEERGIAKGP
                     DGKVMHAEVKIGDSIIMLSDEFPDFGCLSPQSIGGSSSGLHIYLENVDAAFDRAVKAG
                     AKVEMPVMDQFWGDRYGKLQDPFGHKWSIATHVKDMSMDEMKKAMDEACAKMQDQMQK
                     TA"
     gene            complement(966..2291)
                     /locus_tag="VEJ00_10130"
     CDS             complement(966..2291)
                     /locus_tag="VEJ00_10130"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HXX01561.1"
                     /translation="MPDADREVQFRRMRETACEIFRSAVAEASISKAFARHLDCDRGV
                     LRVREDLFDLHSYSRVLVISLGKAGHTMVEALAERVGASLEGIVASPVQPAAQVHGFR
                     YFRGGHPTPNAESVQAASAMLRAVEAQPATALVIFLLSGGGSSIVEKPIDDEITLDDL
                     IATYRALVHSGAPIAEINAVRKHLSAVKGGRLARAAFPAQQVSILVSDVPEATPDALA
                     SGPTMPDSTTVEDCARIAERYDLLKGFPASTRELFERHALEETPKSDDAAFQRARWWT
                     VLSNQTAIDEASAAAERAGFIVHVDNSCDDWDYERAADHLVRRVRELRKQFSPVCLIS
                     GGEVTVRVRNGGVGGRNQQFALACAQKIAGEDVAVLSAGTDGVDGNSPAAGALVDGST
                     LERCKTQGVNALAALEKFDAYPLFKALGDTIETGPTGNNLRDLRILLAR"
     gene            complement(2416..3090)
                     /locus_tag="VEJ00_10135"
     CDS             complement(2416..3090)
                     /locus_tag="VEJ00_10135"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXX01562.1"
                     /translation="MIAANSSIRSLFLDGPVGRLEALLNAGSENATHAALVCHPHPLF
                     GGTLHNKVVFHTMKALNSFGFPVLRFNFRGTGLSQGEHDQGEGEVEDVGAALDWLESE
                     FHLPLVFAGFSFGAAVGLRAACPHKRVRAVIGVGTPVGPVEAESEVPRTYQFDFLPNC
                     MKPKLFVSGARDQFGPRAKLEALVASVPEPKKLVLIEGADHFFEGRLRELRDAIETWV
                     REQVIP"
     gene            3207..4274
                     /locus_tag="VEJ00_10140"
     CDS             3207..4274
                     /locus_tag="VEJ00_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXX01563.1"
                     /translation="MDAVTLTRQLIDIESITGNEATVGNYLFGELRQLGYHTIKIPVE
                     ADRFNVYAVPETESRKPEAAPPPVTFSTHMDTVPPFIPSSEDAERIYGRGSCDAKGII
                     AAQIAASERLRRDGIHVGLLFVAGEERDSLGAKAANEYAANQPKAAMQAPRFLINGEP
                     TENKLALASKGTLRVELAAKGRMAHSAYPELGDSAIDKLLAALTRLRAMPLPSDSEIG
                     PCTLNIGQIEGGRAPNVIPDYAHADLLYRLVGASDELRRQIVATAGNQVEVTFPLEIP
                     FQRLRTVDGPPTMIAAFTTDIPFLGNWGEPLLIGPGSIHVAHTDREHIEKRQLAEAID
                     LYCRISKVLVARTRSEAAANR"
     gene            4438..>4622
                     /locus_tag="VEJ00_10145"
     CDS             4438..>4622
                     /locus_tag="VEJ00_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01564.1"
                     /translation="MLDQAKISEILQAARDKAETILDLRDKARAQRAQALEANQKKMA
                     LQIRLAAPGVESAATVET"
BASE COUNT          947 a         1585 c         1277 g          813 t
ORIGIN      
        1 acaacccgcc gcagttccgc aacgtccacg ccgacccctc cgacgcgacc cgagccttcc
       61 tcgatctcaa ggcgcgctgg atggtcccca tgcattacgg caccttccgc ctgtcgcacg
      121 agccggtcga cgagccgctg cagttgctcg accgcgaagc ccgtgccgcc ggcattcagg
      181 accgcgtggt ggtgatggaa gaaggcgtca cccggttctt ctgagactcc gctgagcctc
      241 gatttttgtc cccagcccgg cctttctccg cgccctctgc gataaaagat cctgacgcag
      301 agcacgccga ggactcacgg agacgacaga gaaagccaga cgcacaattc ggtccttttt
      361 gatgtatact cctttcgcca aatcttcgtc ccaggagaaa atcatgccag tgaagcccat
      421 tcccgacggt taccacaccc tcacaccctt cctcacggtc cgcgacgccg cccgggccat
      481 cgagttctac aaacaggcct tcggcgccga agagcgcggc attgccaagg ggccagacgg
      541 caaagtgatg cacgccgaag tgaagatcgg cgactccatc atcatgctga gcgatgaatt
      601 ccccgacttc ggctgcctgt caccgcagtc gatcggcgga tcatcgtccg gtttgcacat
      661 ttatctcgaa aatgtcgatg ccgccttcga ccgcgccgtc aaggcaggcg cgaaagtgga
      721 aatgccggtc atggaccagt tctggggcga tcgctatggc aagctgcagg acccgttcgg
      781 ccacaaatgg tccatcgcca cccacgtcaa agacatgtcg atggacgaaa tgaagaaagc
      841 tatggacgaa gcctgcgcca aaatgcaaga ccagatgcag aaaacggcgt aagaagctga
      901 atcgggtgcc ccatttctcg cgcgttttct acgcgagaag tggggatttt tttatcccgg
      961 gaaaatcacc gcgccaacag aattctcaaa tcgcgcaggt tatttcccgt tggccccgtc
     1021 tcgatcgtgt cgcccagcgc cttgaacagc gggtaagcat cgaacttttc cagcgccgca
     1081 agggcattca ccccctgcgt cttgcagcgc tcgagcgttg agccgtccac caacgccccc
     1141 gctgccggac tattgccgtc cactccgtcg gtcccggcgc tgagcacggc gacgtcttcg
     1201 cctgcgatct tctgtgcaca ggccagcgca aactgctggt tgcgaccgcc cactccgccg
     1261 tttctgactc ttaccgtgac ttccccgccg gaaatcaggc acaccggcga gaattgtttg
     1321 cgcaattccc gcacccgccg caccagatga tctgccgccc gctcataatc ccagtcgtcg
     1381 cacgagttat cgacatggac gatgaacccg gcccgctcag cggccgcgct cgcctcgtcg
     1441 atcgctgtct ggttcgacag cactgtccac cagcgtgccc gctgaaacgc agcgtcgtcc
     1501 gacttgggcg tctcctcgag cgcatgccgt tcgaaaagct cgcgggtcga ggcgggaaac
     1561 cccttcagca agtcatacct ctctgcgatg cgcgcacagt cttccacagt cgtcgagtcg
     1621 ggcatggtcg gcccggaggc cagcgcatct ggggtagcct cgggcacatc agacacgaga
     1681 atggaaacct gttgcgcggg aaacgccgcc cgcgccagcc gcccgccttt caccgccgaa
     1741 agatgcttgc gcacggcgtt gatctcggcg atgggcgccc ccgaatgcac cagcgcgcgg
     1801 taggtcgcga tcaaatcatc gagcgtgatc tcatcgtcaa tgggcttttc aacgatcgac
     1861 gatccgccgc cgctcagcaa aaatatgacg agcgccgtcg ccggttgtgc ctccaccgcc
     1921 cgcagcatcg cactcgccgc ctgcacagat tcggcgttgg gcgttggatg cccgccgcga
     1981 aaataacgga acccatgcac ctgcgccgcc ggttgcaccg ggctggccac gatcccttcc
     2041 agcgacgccc cgacccgctc cgcgagcgcc tcaaccatgg tgtgcccggc cttgcccagc
     2101 gagatcacca gcacgcgcga atacgaatgc agatcgaaga gatcttcgcg cactcgcagc
     2161 acgccgcggt cgcagtccag atgccgcgcg aaggctttgg agattgacgc ctcggcaacc
     2221 gcggagcgga agatctcgca ggccgtttca cgcatccggc ggaattgcac ttcgcgatcg
     2281 gcgtcaggca tgcgtgggat tataaagaca gcgctagatg acaaacagcc aggagagtta
     2341 gtttggcgtt tctttgcgat ctttgcggat ttttctcggc gaactctgcg gtacaaaagt
     2401 ttttcaacgc aggactcacg gaatgacctg ctctcgcacc cacgtctcga tcgcgtcccg
     2461 cagttcgcgc agccgtccct caaaaaaatg atcggctccc tcgatcagca cgagtttttt
     2521 cggctcggga accgacgcca ccagcgcctc cagtttcgcc cgaggcccaa actggtcgcg
     2581 cgctccgctc acgaacagct ttggcttcat gcagtttggc aagaaatcga actggtaggt
     2641 gcgagggacc tcgctctccg cctcaaccgg accaaccggc gtgcccacgc cgatgaccgc
     2701 acgcacccgc ttgtgggggc acgctgcgcg caggccaacc gcagcgccga aagagaatcc
     2761 tgcaaacacc agcggcaaat gaaattccga ctcgagccag tcgagtgccg cgcccacatc
     2821 ttcgacctcg ccttccccct ggtcgtgctc accctgactc aaacccgtcc cgcggaaatt
     2881 gaaccgcagt acgggaaatc caaaactgtt cagcgccttc atggtgtgaa acacgacctt
     2941 gttgtgcaga gtcccgccaa acagcggatg aggatgacag accagcgcag cgtgcgtggc
     3001 gttctcggag cccgcgttga gcagggcctc cagccgcccg acaggaccat cgagaaacag
     3061 cgagcgaatc gaggagttcg cggcgatcac ctcatcagat tacacaaggc catcggggag
     3121 aagatagccc ggtgcttcgg ccgacgcctg agacccgacg cctgcagccc taaatccggc
     3181 gctcgcgctc tgctattctc tcctccatgg acgccgtcac gctcacccgt cagctcatcg
     3241 acatcgaatc gatcaccggc aacgaagcca cggtcggcaa ctatctgttc ggcgagttgc
     3301 gccagctcgg ctatcacacg ataaagatcc cggtcgaagc cgaccgcttc aacgtctacg
     3361 ctgttcccga aaccgaaagc cggaagccgg aagccgcgcc accccccgtc accttttcca
     3421 cgcacatgga caccgtccca ccgttcatcc catcgtcgga agacgccgag cgtatctatg
     3481 gccgcggctc ctgcgacgcc aagggaatca tcgctgccca aattgccgcg tccgagcgcc
     3541 tgcgccgcga cggaattcac gttggcctgc tcttcgtcgc gggcgaagag cgcgacagcc
     3601 tgggagcgaa agcggccaat gaatatgccg caaaccagcc taaggccgcc atgcaggcac
     3661 cgcggtttct catcaatggc gagcccaccg agaacaagct ggcgctggcc tcgaaaggga
     3721 cactgcgcgt ggagctcgcc gccaaaggtc gcatggcgca ctcggcctat cccgaactcg
     3781 gcgattcggc catcgacaag ttgctcgccg ccctcactcg tctgcgcgcc atgcccctgc
     3841 catccgattc tgagatcgga ccatgcacgc tgaacatcgg acagatcgaa ggcggccgag
     3901 ctcccaacgt gattcccgac tacgcccatg ccgacttgct gtaccgtctt gtcggcgctt
     3961 ccgacgaact gcgccggcag attgtcgcta ccgccggcaa ccaggtggaa gtcacattcc
     4021 cgcttgagat tccttttcaa agacttcgca ccgtcgacgg tccgcccacg atgatcgccg
     4081 ctttcactac ggacatcccc ttcctcggca attggggcga acccctgctc atcggtcccg
     4141 gatcgattca cgtcgcccac actgaccgcg agcacatcga aaagcggcaa ctcgccgagg
     4201 cgatcgatct ctactgccgg atctcaaagg ttctcgtcgc gcgcacaagg tcagaggctg
     4261 cggcaaaccg gtaacacggc tccggcctgc cggtcaaaaa cctgatttac gctctccgga
     4321 cagattgcag ccgcttctcc tgcgccgcgg gattgggcgt atacttccgc tccgcgcgga
     4381 cacatggacg cgcgcccgtc cccacaaccg cacgagttcc gcgatcagga ggacaccatg
     4441 ctagaccagg ccaaaatctc ggagattctg caggcggcgc gagacaaagc ggaaaccatc
     4501 ctggatttgc gcgacaaagc ccgtgcccag cgcgcgcagg ccctcgaggc caatcaaaag
     4561 aagatggcgc tgcagattcg gctggcagcg cccggcgtgg agtcggctgc gacggtcgag
     4621 ac
//
LOCUS       DASPUL010000421         4819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_755309, whole genome shotgun sequence.
ACCESSION   DASPUL010000421 DASPUL010000000
VERSION     DASPUL010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755309"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2794
                     /locus_tag="VEJ00_10150"
     CDS             <1..2794
                     /locus_tag="VEJ00_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01565.1"
                     /translation="STQGGSLSVNGAREQSNNFLLDGVDNNDPYINQYVALPSVDAIQ
                     EFKVQSSDYSAEYGRASGAQINVVLKSGTNAFHGTLFEYFRNRSMDAKNYFDFPNCDA
                     TSTPGECGSIPSLQRNQFGGTLGGPVRKDKTFFFLSYEGLRLRQAQTREATVPSFTQC
                     ATALGVSMGLGGPSSPVPICQPAPNQFPSTLYPAANIGENTFHTAPVIRQTENYYSAK
                     VDQHFSATDTVSAHYSLVDNNVFSPFDPVNAFTALPGYGSFTLNHGQNAGLEWTRVFH
                     SKLLNEFRLGFVRMRATVLQQNHGTDFNAQLGFPDVLTIPVDLGYPDISTNGFDGIGE
                     PVNYPQDRHDTTLQLADNVAWTVGRNQFKIGADVHRVRIDNYLDFLARGEWLFLGCTF
                     TGSSTCQPPDGLDLPTLSLAELWSDIPDAAIGVSGNTFNSLRSHGVSTYVQDDIHVVP
                     RFLLNVGLRYEYNTPPVEALNRFSVPNLVPCRGSIFAPCTTVQPTFTVAGTNGIPRST
                     YSPSKTDFGPRIGIAWRPLKTERWVVRSAYGIFYDSSIANINVFPRINPPFYDLSLHE
                     NGSCPPVGLCTVQDILSTASGLVQDNMISPKFRDGYMQQWNADLQYEVLPDWMVDAAY
                     VGSKGTHLDDVIDRNQLDPTTGISPFGQFASILFVESNATSSYNSLQLRSEKRTRQGL
                     SLLLAYTYSKSFDDLSSVFAGSVGSGLPQNSNDLVAERGPSDFNAVNRFSGSFVYDLP
                     FHRLWANGPGWSERLLNNWQAGSIITAQSGSPFTVVLSGGASSAAAAFGNPARPDLVA
                     SPFQPGPVAANPGCIYTPATEVRMPQSWFNPCAFAMPSSGPTGAPLFGTEGRNVLTGP
                     GFTDLDFGLSKSMALGSESHRLTIRGDFFNLFNHPNFDVPNHVFGTPNFAQVLSANFY
                     GSKPPRQIQLSMRYVF"
     gene            complement(2800..4005)
                     /locus_tag="VEJ00_10155"
     CDS             complement(2800..4005)
                     /locus_tag="VEJ00_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXX01566.1"
                     /translation="MPDVRPLTFLCITTYEKGQEFMRQCKRQGCRVILLTAEKLRNAD
                     WPRESLDDTFYLPAEISAADIVQAVTHLARSQKLDRIVALDEYDMETAASLREHLRIP
                     GMGLTTMRYFRDKLAMRMRALDRGLRVPDFVPAVNHDDIRYYCEHVAGPWVLKPRQEA
                     SAIGIKKIQTADELWPALEPLGDKQSSFLLEKFVPGEVYHVDSVVSENEVVFANVSKY
                     GKPPMNVAQGGGIFTTFTVPRGGAADSALRAINRDLIAALGLVRGVAHAEFIHAQADG
                     QFYFLECAARVGGAYINEMVEAATGINLWREWARIEVAGGDGSYKLPPVREDYAGVII
                     SLARQENPDTSAYNDPEVQLRIKKHHHAGLVLHSKEPSRIAALLESYAQRFAEEFLAV
                     EPQREKPTA"
     gene            4090..4572
                     /locus_tag="VEJ00_10160"
     CDS             4090..4572
                     /locus_tag="VEJ00_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01567.1"
                     /translation="MLDCRTMVEEIKRWNFDVMWALLLALGALAANVAVFATPPLQSA
                     LPWVSLCLGILALIVLVRGLWRAFGESQIYRGKVLSVVLGVLTVALAGLMVFAFFLAR
                     KLPTSVDAPQVGQQVPDFTLTDSNGKAVSLDSLFAAESSEPQAAAPKAVLLIFYRGYW
                     "
     gene            4612..>4819
                     /locus_tag="VEJ00_10165"
     CDS             4612..>4819
                     /locus_tag="VEJ00_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXX01568.1"
                     /translation="MADFKAAGVRPVAISVDVPQISLDLSRKVGYTFPILSDPDAAVI
                     RSYHLLHAGGGPEGHDIARPAEFLV"
BASE COUNT          928 a         1485 c         1378 g         1028 t
ORIGIN      
        1 ctcgacgcag ggtggatcgc ttagcgtcaa cggagcccgc gagcaatcga ataatttcct
       61 gctcgatggg gtggacaaca acgatcctta catcaaccag tacgtggccc tgccctcggt
      121 ggatgccatt caggagttca aagtccagtc cagcgactat tcggccgagt acggacgggc
      181 cagcggcgct cagatcaacg tcgtgctgaa gagcgggacg aacgccttcc acggaacgct
      241 gttcgagtac ttccgcaatc gcagcatgga cgccaagaac tacttcgact tccccaattg
      301 cgatgcgact tcgactccgg gggagtgcgg aagcattccg tcgctgcagc gcaatcagtt
      361 cggcgggacg ctgggcggtc ccgtgcgcaa ggacaagact ttcttctttc tttcgtatga
      421 aggattgcgc ctgaggcagg cgcaaacacg cgaagccacc gtgccgtcat tcacgcaatg
      481 cgcgactgcg ctgggcgtat ccatgggact gggcggcccg agttcgcccg tacccatttg
      541 ccagcccgcg ccaaaccagt tccccagcac tctttacccg gcggccaata ttggcgagaa
      601 cacattccac acggccccgg tcatccgcca gacagagaac tactattccg ccaaggtgga
      661 tcagcacttc agcgctaccg atacggtatc cgcccactat tctctggtgg acaacaacgt
      721 cttcagcccg ttcgatccgg tgaacgcctt cacggctctg ccgggctacg ggagcttcac
      781 actcaatcat ggtcagaacg cgggactcga gtggacgcgc gtgtttcact ccaagctgct
      841 gaatgaattt cgcctcggtt tcgtccgcat gcgggcaacg gtgctgcagc agaatcacgg
      901 cacggacttc aacgcgcaat tgggcttccc ggacgttctc accatcccgg tcgatctggg
      961 gtatcccgac atttcgacga atggctttga cggcatcggc gaaccagtga actatccgca
     1021 ggatcgtcac gacacgaccc tgcaactggc agacaatgtc gcctggacag tcggccgcaa
     1081 tcaattcaag ataggagcgg acgtacaccg cgttcggatt gacaactacc tagacttcct
     1141 cgcccggggc gagtggctgt tcctgggatg taccttcacc ggatcgtcca cctgccaacc
     1201 tcccgatggc cttgaccttc cgactctgtc gctggcggag ttgtggtctg acattcccga
     1261 tgccgccatc ggagtgagcg gcaacacttt caacagcctg cgcagccacg gagtcagcac
     1321 ctatgtgcag gatgatattc acgtggttcc tcggttcctg ctgaatgtcg ggctgcgcta
     1381 cgagtacaac acgccgccag ttgaggctct gaaccggttc agcgttccca acctggttcc
     1441 ctgtcgagga tctatcttcg ccccttgcac caccgtccaa cccacattta cggttgcggg
     1501 cacgaacggg atcccgcggt caacgtactc tccctctaaa actgacttcg gcccgcggat
     1561 cggtatcgcc tggcgcccgc tgaagaccga gcgctgggtg gtgcgctctg cctatggcat
     1621 cttctacgat tcgtcgatcg caaatatcaa tgttttcccc cgcatcaatc cgccgtttta
     1681 tgacctgtcg ctgcatgaaa acggcagctg ccctcctgtg ggattgtgca ccgtacagga
     1741 tatcttgagc acagccagcg gcctggtgca ggacaacatg atttctccca agtttcgcga
     1801 cggatatatg cagcagtgga acgccgactt gcagtacgag gtgttgcccg actggatggt
     1861 cgacgccgct tacgtcggct cgaaagggac ccacctggat gacgtgattg atcgcaacca
     1921 gctggacccc acaaccggca tatcgccctt cgggcagttc gcttccattc tttttgtgga
     1981 gtccaatgca acaagttcct acaattcgct gcagttgcgc tcggagaaac ggacgcggca
     2041 aggactgtcg cttctgttgg cctacaccta ctccaagtca ttcgacgacc tctcgtcggt
     2101 gttcgcgggg agcgtcggtt ccggcctgcc gcagaattcg aatgatctcg tcgccgagcg
     2161 cggtccgtcg gacttcaacg ccgtaaatcg tttctcgggg agcttcgtgt acgatctgcc
     2221 gtttcatcgc ctgtgggcga acggccccgg atggagcgag agacttctga acaactggca
     2281 ggcaggaagc atcataaccg cccagagcgg ctcgcctttc acggtggtac tttcgggggg
     2341 agcttcctcc gctgccgcgg cgtttggcaa tccggcccgg ccggatcttg tagccagccc
     2401 gttccagcct ggccccgtcg cagccaaccc tggctgcatc tacaccccgg cgactgaggt
     2461 gcgcatgccg cagagttggt tcaatccctg cgcctttgcg atgccttcca gcgggccgac
     2521 cggcgctccg ctttttggga ccgaaggacg aaacgtattg accggcccgg gtttcactga
     2581 tctggacttc ggtttatcga aaagcatggc cctaggtagc gagagccaca ggctaaccat
     2641 ccggggggat ttctttaacc tgtttaatca tcccaacttt gatgtcccca atcacgtttt
     2701 cggcactccg aacttcgccc aggtgctctc cgctaacttc tatggaagca aaccaccacg
     2761 ccagattcag ttgagtatga ggtatgtctt ctagtccatc taagccgtcg gcttttcgcg
     2821 ctgaggttcg acggcgagaa actcttcggc gaagcgctgg gcgtagctct ccagcaaagc
     2881 tgcaatccgg ctcggttcct ttgagtgcag caccagcccc gcgtggtgat gcttcttgat
     2941 gcgtagctgc acttcaggat cgttgtaagc agaggtgtca ggattctcct gccgcgcgag
     3001 ggagatgatc acgccggcgt agtcttcgcg aaccggcggc agcttgtagc tgccatcacc
     3061 gcccgccact tcaatgcgcg cccactcgcg ccacagattg attccggttg ccgcttcgac
     3121 catctcgttg atgtaggcgc ctccgacgcg agcggcacat tcgaggaaat agaattgccc
     3181 gtcggcctgc gcatgaatga attctgcatg cgcgactccg cgcaccaggc ccagcgcggc
     3241 gatcaagtcg cggttgatgg cgcggagcgc cgaatcggcc gctccgcccc gcggcacggt
     3301 aaatgtcgta aagattcccc cgccctgggc cacgttcatc ggaggcttgc cgtacttgct
     3361 gacgttggcg aagacgacct cgttctccga cacgaccgaa tccacgtggt acacctcgcc
     3421 cggcacaaac ttctcaagca agaatgagga ctgcttgtcg cccagcggtt cgagtgcggg
     3481 ccacagttcg tcagcagtct gaattttctt gattccgatc gccgacgctt cctgccgcgg
     3541 cttaagcacc cacggccccg caacgtgttc gcagtagtac cggatatcgt cgtggttgac
     3601 cgcaggcaca aagtccggca cgcgcagtcc ccggtcgagc gcgcgcatgc gcatggccag
     3661 cttgtcgcgg aaatagcgca tcgtcgtaag gcccatgccc ggaatccgca gatgctcgcg
     3721 caagctagcg gcagtttcca tgtcgtactc gtcgagggcg acaatgcggt cgagcttttg
     3781 gctgcgtgcc agatgagtta cggcctgcac gatatcggcg gcggagattt cggcgggaag
     3841 gtaaaaggta tcgtcgaggc tttcccgggg ccagtcggca ttgcggagct tttcagcagt
     3901 cagcagaatc acgcggcagc cctggcgctt gcactggcgc atgaattcct gacccttttc
     3961 gtaggtcgtg atgcagagga acgtcagggg gcggacatct ggcatggctg catttaaatc
     4021 agggctagga gaataccacg gccggacgca aagctcgcat cctcgaattg acaaaacccg
     4081 aagctctctt tgctagactg cagaacgatg gtcgaggaaa taaagcgttg gaacttcgat
     4141 gtgatgtggg cgctgctgct cgcgcttggg gccttggcag cgaacgttgc ggtattcgca
     4201 actcctcctc tgcaatcagc gctcccctgg gtgagtctgt gtctgggcat cctggcgctg
     4261 attgttctgg ttcgcggatt gtggcgagcc ttcggagagt cgcagattta tcgaggcaag
     4321 gtgctgagcg ttgttctcgg cgtgctcacg gtggcgcttg ccgggctgat ggtcttcgcc
     4381 tttttcctgg ctcgcaagct ccccacttcg gtggatgccc cgcaggtcgg tcagcaagtt
     4441 ccggatttca cgctgaccga cagcaatggc aaggccgttt ccctcgacag cctcttcgca
     4501 gccgaatcca gcgaacccca agccgcggcg ccgaaggcag tcctgttgat cttctatcgc
     4561 ggctactggt gaccttactg caacctcgag ttacgagggg tcgagaaacg actggcagat
     4621 ttcaaagcag caggcgtgcg tccggtcgcg atcagcgtag acgtgccaca aatctcactc
     4681 gatctcagca ggaaggtggg ctacaccttc ccaattctct ctgatcctga tgccgctgta
     4741 atccgcagct accatctgct ccacgcgggt ggagggcctg agggccacga catcgcgcgc
     4801 ccggcagaat ttctggtcg
//
LOCUS       DASPUL010000422         4968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1997527, whole genome shotgun sequence.
ACCESSION   DASPUL010000422 DASPUL010000000
VERSION     DASPUL010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997527"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1632)
                     /locus_tag="VEJ00_10170"
     CDS             complement(<1..1632)
                     /locus_tag="VEJ00_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01569.1"
                     /translation="MLLSVVLHLATPGLAQGQTAQEQPAAVLAASGTQSGRLTRIYPM
                     RDGNRASVKPGRFHTANGDATASAATTGGPDLQYYGGPVISNAQVVVVYWGNSVSSVA
                     TSGIPAFYSAVTNSNWMDLLSEYNTSGVTPVGGSAAGDQAIGRGFVSGVYTITPSAAN
                     SGSTVDDTQIQAEIVAQINAGHLPAPATDNTGAITTLYMVYFPPGATITLQGASSCAE
                     FCAYHGTTSYNNLDLPYGVLPDLGPGTACSTGCAAGTEFEDLTTVSSHEMAESITDTG
                     VGLATVLGPPLAWYDVNYGEVGDICNASGVPANQAVLPGSGYTVQKLWSNLQQDCVSA
                     PPVFTVSAPASVSSGTPFTITLSVQSSTGATLSPPYSGTVEFTSSDSSALLPANYTFT
                     ATDSNSHTFSNAATLKTAGAQTITVTDTHSGGFSGSAQVNVTVHTSPYFSLAVPSAAI
                     LGTPFSFTVTVLTPSGSLNTTYSGTAHFSSSDPQAVLPANATLTNGTGTFSATLHTPG
                     SQTITATDTVNSAITGSSPGIAASGAATHFAVVAPA"
     gene            complement(2040..2291)
                     /locus_tag="VEJ00_10175"
     CDS             complement(2040..2291)
                     /locus_tag="VEJ00_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXX01570.1"
                     /translation="MTPEAQQMIEHIAGLIRRPGVNIDENTPLVSSGLIDSMALVDLL
                     LRLEDLTHMRIPAGKIQPKDLDTVAKMFATAQRVGKPRR"
     gene            complement(2335..4041)
                     /locus_tag="VEJ00_10180"
     CDS             complement(2335..4041)
                     /locus_tag="VEJ00_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXX01571.1"
                     /translation="MLGYSNLVAALEAAPADRLFVRAWVDEDEQLSRTFGEFRAQARA
                     QAAILRDHGVAASDRVVIIMPQGIAAMATFAGAMMLGAVPAFLAYPNFKVEPAKYRSG
                     LAGVTANLRASTVVIDEEFPPEMLGHVTVSDGTKLLRAGGGAGGGERELVEFDAPDIR
                     ADGVAFIQHSAGTTGLQKGVALTHAAVLRQIQHLARALRIDGAKDRIYSWLPLYHDMG
                     LIACFMLPMVCHVAVVMQSPLDWVMYPETMLEIISRHRCTLGWMPNFAFQFVPRRTPP
                     QRRAQHDLSSLRALINCSEPVRATSMREFQSAFAAAGLKENTLQSSYAMAENVFAVSQ
                     SDITRRDGPPCIWADGQQFRSAHRIVPVPESTPGAMAFMSSGRLLPDHEVCIASDFGA
                     ALGEGNVGEILVRSDCLFAGYYNRPDLTAQAIVGGWYHTGDLGFMLDGELYVVGRKKD
                     LLIIGGENIYPQDIEEIVAGHPAVHDGRAIAMGVYNADLGTEEIVVVAELEGENLLAR
                     SAEIEQEIRTRVVAGMGVAAKVIFLKPPKWIVKSTAGKAARSATREKLLREHPELNAE
                     IA"
     gene            4271..>4968
                     /locus_tag="VEJ00_10185"
     CDS             4271..>4968
                     /locus_tag="VEJ00_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01572.1"
                     /translation="MLQAILYGLLFLLCAVVLARVRSRSVRQDVLLIASLTLYLTWGF
                     WFVAVLLASTVMNFLVGRWLRRNASGVVLTLGILLNLALLGTFKYLPEAALCLPFASL
                     ERFSHLALPLGISFWTFQAMSYLFDLYRGDELDPSFFEFALYMAFFPVTISGPICRLP
                     DMLPQFRSEARTAWNDITRGFRRIATGVLMMQLAKLLGQGILSGDGISSGFDRVTHWG
                     GADVWCLALGYGLQL"
BASE COUNT          861 a         1573 c         1524 g         1010 t
ORIGIN      
        1 agccggcgcc actacggcaa agtgcgtcgc cgcgccggaa gccgcgatgc cgggcgagga
       61 gccggtgatc gcgctattca cggtgtcggt cgcggtgatc gtctggctgc cgggggtgtg
      121 cagcgtggcg ctgaaggttc ccgtgccatt ggtcagggtg gcgttggcgg gcaaaaccgc
      181 ctgcgggtcg gagctcgaga aatgtgcggt gccgctgtat gtcgtgttga ggctgccgga
      241 aggcgtcaac acggtcaccg tgaagctgaa tggcgttccc aggatcgccg ccgagggcac
      301 ggccagggaa aagtatggcg aagtatgaac cgtgacattg acctgcgccg atccgctgaa
      361 gcccccggag tgcgtgtcag tcacggtgat ggtctgggcg cccgcggtct tcaaggtcgc
      421 ggcgttcgag aatgtgtgcg agttggaatc agtcgccgtg aaggtatagt tcgcgggcag
      481 aagcgcgctg ctgtcgctgc tcgtgaactc gaccgtgccg ctgtagggcg ggctgagagt
      541 cgcgcccgtg ctgctttgca ccgagagtgt gatcgtgaac ggagttccag atgacacgct
      601 cgccggcgcc gacacggtga aaaccggcgg ggcgctgacg cagtcctgct gcaagttgga
      661 ccacagtttc tgcacggtgt agccgcttcc cggcagaacc gcctggttgg cgggaacgcc
      721 gctggcattg cagatgtcgc cgacctctcc gtagttgacg tcgtaccagg ccaatggcgg
      781 cccaagaact gttgccaaac cgactcccgt atctgtaatg gactccgcca tctcgtggga
      841 cgaaaccgtg gtaaggtcct cgaactctgt gcctgccgca cagccggtcg agcaagccgt
      901 gcccgggccc aggtcgggca gaacgccgta ggggagatcg aggttgttgt agctcgttgt
      961 gccgtggtag gcgcagaact ccgcgcagct tgaggcgccc tgcagggtaa tcgtggcccc
     1021 cggcgggaaa tagaccatgt agagcgtagt aatggctccc gtgttgtcgg tagccggcgc
     1081 gggcagatgc ccggcattga tctgggcgac gatctccgcc tgaatctgtg tatcgtcgac
     1141 agtgctgccg gagttggccg cggacggcgt gatagtgtag acgccgctga cgaagccgcg
     1201 tccgatcgcc tggtcgcccg ccgcgctccc gcccaccgga gttactccac tggtgttgta
     1261 ttcggagagc aggtccatcc agttgctgtt ggtgacggcg ctgtagaagg cggggattcc
     1321 gctcgtcgcg accgagctga cgctgttgcc ccaataaacc acgaccacct gcgcgttcga
     1381 gataaccggg ccgccgtagt actggagatc ggggcctcca gtagtcgcgg cgctggccgt
     1441 cgcgtcgccg ttggccgtgt gaaacctccc cggcttgacg ctggcgcgat tcccgtcgcg
     1501 catgggatag atgcgcgtca ggcgcccgct ttgagtgcca ctcgccgcta ataccgccgc
     1561 gggttgctcc tgcgctgtct gcccctgggc aagccccggg gtggcaagat ggagcactac
     1621 ggatagcagc accaccggcg agattccctg gactgggcta ttgccaaata ttgacgacac
     1681 actgcggcaa tcttgaggcg cgcgccactt catacgagct taccctccct gaaccggaca
     1741 agagcattgg aagacgccgc ctgagatcaa gcaccgctaa gattcttcga gtaaacctcg
     1801 cccccttgtt gcgcaaagcc agccgaatag agctgtacct gcccggcgca ccaggaaagc
     1861 agctattatg attggtgaag ccccataacc tcggagtgag gaatccccga agcgttcagc
     1921 ggattatgga ggatttcggg cgcgctgtca tcaacgaaag tgcgaaattc gtttggcccc
     1981 gttcctaatt tgggtcacac ccggaagaat cgttgctaat tctctcaccc aggctcgcgc
     2041 taacgcctag gttttccgac ccgctgcgcg gtggcgaaca tcttggccac cgtgtccaga
     2101 tccttcggct gtatctttcc cgccgggatg cgcatgtgag tgaggtcttc gagcctcaac
     2161 agcaggtcga ccagcgccat cgagtcgatc agcccggaag aaaccagtgg cgtgttctcg
     2221 tcgatgttca cgcctggcct gcgaatcaac ccggcgatgt gttcgatcat ctgctgcgct
     2281 tctggcgtca tgctggcgat ccacttctgg aatctgatga aaaccggtcg ccggctacgc
     2341 aatctcggcg ttgagttccg gatgctctcg caacaatttc tcgcgtgtgg cggaccgcgc
     2401 cgccttccct gcggtgctct ttacaatcca cttcggcggc ttgagaaaga tcacttttgc
     2461 cgccactccc ataccggcaa cgacgcgcgt gcgaatctcc tgctcgatct cggctgaccg
     2521 tgcgagaaga ttctcgccct ccagttccgc caccaccaca atctcttcgg tcccgagatc
     2581 ggcattgtac acgcccatgg ctatcgcgcg gccgtcgtga accgcgggat ggccagcgac
     2641 gatctcttca atgtcctgcg gataaatgtt ttcgccgccg atgatcagca ggtctttctt
     2701 gcgcccgacg acgtagagtt cgccatccag catgaagcca aggtcgccgg tgtgatacca
     2761 gccaccgacg atggcctgcg ccgtcaggtc gggccggttg tagtagccgg cgaacaggca
     2821 atcgcttctg accagaatct cgcccacgtt cccctccccg agcgccgcgc cgaagtcgga
     2881 cgcaatgcag acctcgtgat ccgggagcag ccgccccgac gacatgaacg ccatggctcc
     2941 cggagtgctc tccggcacgg gcacgatgcg gtgcgcgctg cgaaattgct ggccatctgc
     3001 ccagatgcaa ggaggcccat cgcgccgggt gatatccgat tggctgacgg caaaaacgtt
     3061 ttccgccatg gcgtaggaag attgcagtgt gttctctttc aatcccgccg cagcaaacgc
     3121 gctctgaaac tctcgcatgc tggtggctcg caccggctcc gagcagttga tcagcgcccg
     3181 cagcgacgaa agatcgtgct gcgctcttcg ctgcggcgga gtgcgccggg gcacaaactg
     3241 gaatgcgaag ttcggcatcc agcccagcgt gcacctgtgc ctgctgatga tctccagcat
     3301 cgtctcggga tacattaccc agtcgagcgg cgactgcatc acaaccgcaa cgtggcacac
     3361 catcgggagc atgaagcagg caatcagccc catgtcgtgg tagagcggca accagctgta
     3421 gatgcggtct ttcgctccgt caatccgcag cgcccgagcc agatgctgga tttgacgcaa
     3481 aacggcggcg tgcgtcagcg cgactccttt ctgtagcccg gttgttcccg ccgagtgctg
     3541 gatgaacgcc acgccgtccg cgcggatgtc cggggcatcg aactcgacca gctctctttc
     3601 gccgcccccc gcgccgcctc cggcgcgcag caactttgtc ccatcgctga cggtgacgtg
     3661 gccgagcatc tcagggggaa attcttcgtc gataaccacg gtgctggccc tcagattcgc
     3721 agtcacgccc gccagcccgg agcggtactt ggccggttcg accttgaagt tgggataggc
     3781 gagaaacgct ggcacagcgc cgagcatcat ggctccggcg aatgtcgcca tcgccgcgat
     3841 cccctggggc atgatgatta ccacgcggtc actggcggct acgccatgat cgcgcaggat
     3901 cgcagcctgc gcgcgagctt gcgcgcggaa ttcaccgaaa gtccggctca attgctcgtc
     3961 ttcgtcgacc cacgccctca cgaagagacg gtcggcaggc gccgcttcga gcgcagcgac
     4021 gagattgctg tatccaagca cacgtcattg tagtgcacgt cgctgagggc ctggccttct
     4081 tgtcacccgt gacgctcccg gctattcttg tcggctgatt cgatcaatca cacgcaacgg
     4141 acgattgcgc ggccgtggaa gagcggcgct ttagcgccgc gtcggcgccg ccaagacaat
     4201 atgggcttta gcccaggtgt tcgtttttgg atccaccaat atccccgctg gaccggctca
     4261 gggaggaacg ttgctccaag ctattctcta cggcctgctg tttctgctgt gcgctgtcgt
     4321 cctcgccagg gtccgttcgc gcagcgtgcg ccaggacgtc ctcctgatcg ccagcttgac
     4381 gctctatctc acctggggat tctggttcgt cgccgttctt ctcgcctcca cggtgatgaa
     4441 cttcctcgtc ggccgctggc tccggcgcaa tgcttccggc gttgtgttga cgctgggcat
     4501 ccttctgaac ctcgcgctgc tcggcacttt caagtacctg ccagaggccg cgctgtgcct
     4561 gcctttcgct tcactcgagc gcttctcgca tctggcgctg cccctcggca tttcgttctg
     4621 gaccttccag gcgatgagct acctgttcga cctctatcgc ggcgacgaac tcgatccgtc
     4681 gtttttcgag tttgccctgt acatggcctt ctttcctgtg acgatctccg ggccaatctg
     4741 ccgcctgccc gacatgctcc cgcaattccg ctctgaagcg cgcaccgcat ggaatgacat
     4801 aacgcgggga tttcgccgca tcgctaccgg tgtgctgatg atgcaactgg ccaaacttct
     4861 cggtcagggc attctgtccg gcgacggtat cagcagtggc tttgaccgcg tcacgcattg
     4921 gggcggcgcc gatgtctggt gcctcgcgct cggctacggc ctgcaact
//
LOCUS       DASPUL010000423         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_485957, whole genome shotgun sequence.
ACCESSION   DASPUL010000423 DASPUL010000000
VERSION     DASPUL010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485957"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /gene="ribH"
                     /locus_tag="VEJ00_10190"
     CDS             complement(<1..428)
                     /gene="ribH"
                     /locus_tag="VEJ00_10190"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXX01573.1"
                     /translation="MKVESAREPSSPALEGALDASGKRFAIVVSRFNAFITERLLQSA
                     CDGLLRSGANKKDIAIVRVPGSFEVPSAARTLAETKKYDAIICLGCLLRGDTAHYDVI
                     VNEVTRGIGQSAQETGVPHAFGVLTCDTLEQAIDRAGLKMG"
     gene            complement(626..1408)
                     /locus_tag="VEJ00_10195"
     CDS             complement(626..1408)
                     /locus_tag="VEJ00_10195"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXX01574.1"
                     /translation="MNFENILLEKKNAIAYVTVNRPKVLNALNMATMEELRAAFHEIK
                     ADKTIRVVIFTGAGEKAFIAGADISELAKHDAVSGKEYTHRGQSVLNLIENLGKPVIA
                     CINGFALGGGCEIAMACTMRLASENAKLGQPEVKLGIIPGYGGTQRLPRLVGKGLAMQ
                     QVLTGEMITAQEAHRIGLVNEVTTAAELIPRAEAIAAKIIANAPLAVQYAIEAVNKGM
                     EMTLAEGLYLEATLFGVCCATEDKKEGTTAFLEKRPAQFKGR"
     gene            complement(1723..2616)
                     /locus_tag="VEJ00_10200"
     CDS             complement(1723..2616)
                     /locus_tag="VEJ00_10200"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HXX01575.1"
                     /translation="MEIKKVGVLGCGLMGSGIAQVCAMAGFDVTVLEVEQKYLDKGFA
                     GIEKSLAKFAERPVDKGGITADQKAATRARLKGTTNKSDLADCDIVIEAIIENVEEKK
                     MMYASLDGIVKKDAIFASNTSSISVTELLTAVKRPERFIGLHFFNPVPLMKLVEVVRT
                     IATADDVYESAYDFAKKLGKVPVRTSDKTGFIVNRLLVPYLLDAIRAYEEGVGSIEDI
                     DNAMKLGCGYPMGPFTLLDFVGLDTTYYITHVMHDEFKERRFASPPLLKRLVMAGWYG
                     RKSGKGFYDYADPEKPRANRL"
BASE COUNT          507 a          815 c          785 g          615 t
ORIGIN      
        1 cccatcttca acccggcgcg gtcgatggcc tgctccagcg tgtcgcaggt cagcacaccg
       61 aatgcatggg gcacgccggt ctcctgcgcc gactgtccga tcccgcgcgt gacttcgttg
      121 acaatcacgt cgtagtgcgc ggtgtcgcca cggagcaggc agccgaggca gatgatggcg
      181 tcgtatttct ttgtctcggc cagagtgcga gcagcagaag gaacttcgaa cgaaccgggc
      241 acgcggacga ttgctatgtc tttcttgttt gcgcccgaac gcagcaaccc atcacaggcg
      301 ctttgcagca gccgttctgt gatgaaagca ttgaagcgag acacgacgat ggcgaaacgc
      361 ttgccggaag cgtcgagtgc cccttccaga gccggggacg aaggctcccg cgcggactca
      421 actttcaatt tgagtttctc tgcgatctct gtgtcctctg cgtgaaatca ggcgcccggg
      481 ttgtcatcct gagcgaagcg aaggacctat gcactctgcc tgcgccggcg aattgcacag
      541 gtccttcgcg gcaactgcgc cgctcaggat gacaagtgaa tgggtttgat ccgcgaaaat
      601 ccgcgtcaat ccgtggctaa gaagttcacc gtcctttgaa ctgtgccggg cgcttttcca
      661 ggaacgcggt cgttccttct ttcttgtcct cggtcgcgca gcagacgccg aacagcgttg
      721 cctccagata cagtccctcg gcaagcgtca tctccatgcc tttgttgacg gcttctatcg
      781 cgtactgcac ggccagcgga gcgttggcga taattttcgc cgcaattgct tcggcgcggg
      841 gaatcaattc ggccgccgtg gtcacttcgt tcacgagccc gatgcggtgc gcctcctgcg
      901 ccgtgatcat ttctccggtg agaacttgct gcattgccag gcccttgccc accaggcgcg
      961 gcaggcgctg cgtgccgccg tagcccggga tgatgccgag ctttacctcc ggctggccga
     1021 gctttgcgtt ctcgctcgcc aggcgcatgg tgcaggccat ggcaatttcg catccgccgc
     1081 cgagcgcgaa tccgttgatg caggcaatca ccggcttgcc cagattctcg atcagattaa
     1141 gcaccgactg cccgcgatgc gtgtactcct tgccgctgac ggcgtcgtgc ttggcgagtt
     1201 cgctgatgtc ggctccggcg atgaaggcct tctcgcccgc gccggtgaag atgacaactc
     1261 ggatcgtctt gtcggcttta atctcgtgaa acgccgcccg cagctcctcc atggtggcca
     1321 tgttgagcgc gttgagcacc ttgggccgat tgacggtgac gtaggcgatc gcgtttttct
     1381 tttcgaggag gatgttttcg aagttcataa attcagtctg ccacggatca gcggattcac
     1441 acgaattatg ccagatgtca tcctgagcga ggattttgct tcgcaatgcg aagcaagacc
     1501 gcagtcgaag gatcccttgc acctcaatcg accgatggcg cggcaaggag ttctctcggc
     1561 ggccgctgtg agaatgcctt gctgcgtccg tcgtcgcagc agatcagagg gatgcttcga
     1621 ctccgcatga tctgcacttc gtgcagctca tgctccgctc aggatgacaa cttctagagc
     1681 cggaatatcg ggtgcaatcc acataaatcc gtgaccaaga agttacagcc tattcgctct
     1741 cggcttttcc gggtcggcgt agtcataaaa tcctttgccc gacttgcggc cgtaccagcc
     1801 cgccattacc agccgcttca gcaacggagg cgaggcgaag cggcgctcct tgaactcgtc
     1861 gtgcatcacg tgcgtgatgt aataagtcgt gtcgaggccg acgaaatcga gcagggtgaa
     1921 cggccccatg ggatagccgc agcccagctt catggcgttg tcgatgtctt caatcgagcc
     1981 gacgccttct tcgtaggcgc ggatggcgtc gagcagatac ggtaccagca gccggttcac
     2041 gatgaatcct gtcttgtccg aggtgcgcac cggaaccttg cccagttttt tcgcgaagtc
     2101 gtaagccgac tcgtagacgt cgtcggcagt cgcgatcgtg cgcacgactt cgaccagctt
     2161 catcagtggc acgggattga aaaagtgcag cccgatgaag cgttccgggc gcttcaccgc
     2221 ggttagcaac tcggtcaccg agatcgaaga cgtgtttgag gcgaagatgg cgtctttctt
     2281 cacgatgccg tcgagtgagg catacatcat ctttttttct tcgacgttct cgatgatggc
     2341 ttcgatgacg atgtcgcaat cggcgagatc ggatttgttc gtcgtgccct tcagccgggc
     2401 gcgggttgcg gctttctggt cggcggtgat gcctcccttg tcgaccggcc gctcggcgaa
     2461 cttggccagg gatttctcaa tcccagcaaa gcccttgtcg aggtatttct gctcgacttc
     2521 gagcacggtg acgtcgaacc cagccattgc acaaacctgc gctattcccg agcccatgag
     2581 tccgcagccg agaacaccaa cctttttaat ctccataatt gcttccttca agcgctgtca
     2641 tcctgagcga ggattgtgct tcgcgagacg cgaagcgcaa ccgcagtcga aggatctctt
     2701 tcacctcaac gaccggtagc ct
//
LOCUS       DASPUL010000424         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_756186, whole genome shotgun sequence.
ACCESSION   DASPUL010000424 DASPUL010000000
VERSION     DASPUL010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756186"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VEJ00_10205"
     CDS             <1..756
                     /locus_tag="VEJ00_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXX01576.1"
                     /translation="NLGATVGSVVLVTSPQGELTPFGMVPKYSRFRVVGIFNSGFFDY
                     DSSWAFARLSDAQRLFGLGDLVSVLEFKVDDLDQADAISQQIENAADKGFMATNALKQ
                     NSALFKALKTERLVTFITIGLIVFVAALNILISLIMMVMEKTKDIAVLISMGTRQSQV
                     RNVFIAQGVLIGVIGTVIGLVVGYAVSYAGGHYHMISLSPEVYSIDYVPFAPRLLDGV
                     IVAVVAIGISFVATLYPSWSASRILPAEALRYE"
     gene            1170..1859
                     /locus_tag="VEJ00_10210"
     CDS             1170..1859
                     /locus_tag="VEJ00_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXX01577.1"
                     /translation="MLRVEGLKKVFRSGESDLVLFENLSFEVKRGEMLAIVGESGAGK
                     STLLHILSALERASAGDVYCAQLRLNSLSQNEASDFRNRETGFVWQFHYLLPEFTAAE
                     NVAMPLLLRGQTWQQAEPEALRWLREVGLEQRAHHRSGELSGGEQQRVALARALITRP
                     KLLLADEPTGDLDGKTAEAVFELIARLHSEHQLTSLIATHNLSFARRCHRVLRLQRGS
                     IEEITPDSLPA"
BASE COUNT          413 a          583 c          622 g          460 t
ORIGIN      
        1 aatcttggcg cgaccgtcgg ttcggtcgtg ctcgtcacca gcccgcaggg cgaactgact
       61 cccttcggca tggtcccgaa atatagccgt ttccgcgtgg tcggaatctt caactccggc
      121 ttcttcgact acgactccag ctgggctttc gcccgccttt cggacgccca acgtctgttc
      181 ggcctcggcg atctcgtctc ggtgctcgaa ttcaaagttg acgatctcga tcaagccgac
      241 gccatctcgc agcagatcga aaacgccgcc gacaaaggct tcatggctac caacgccctg
      301 aagcagaaca gcgccctgtt caaagccctc aagacggagc gcctggtcac ctttattacc
      361 atcggcctca tcgtgttcgt ggctgccctt aacatcctca tcagcctcat catgatggtc
      421 atggagaaaa ccaaagacat tgccgtgctg atatcgatgg gaacgcggca gtcccaggtg
      481 cgcaatgtgt tcattgccca gggcgtgctg atcggcgtga tcggtaccgt gatcggttta
      541 gtcgtcggct acgccgtctc ctacgccggc ggccactacc acatgatttc cctctcgccc
      601 gaggtctatt cgatcgatta cgtgccgttc gcgccccggc tgctcgatgg cgtcatcgtc
      661 gccgtggtcg caatcgggat ttcgttcgtt gcgacgctct atccgtcgtg gtccgcatcc
      721 agaatcttgc ccgccgaggc cttgcgctat gagtagcgag tggcgagcta cgagtgtcgg
      781 gcatcggacc gattccgcgt ccgctgcttt cggggatgtg ggggtcccaa cgcgatagac
      841 ccgagtgaca ggaactcgtg gaagagcggc ccttcagggc cgcgtcacgg gcggagatcc
      901 gtggggcttc agccccggtg gtcattgttg ggtgaaagcg ctagtggccg tgactccggt
      961 cacatgcagg aaaccggaat ctatgtacag ttctgggaag gcaaacctac tgtgaaatgc
     1021 gcacgtgtcg aaaccatctt ccctctggcg agtctaatgc aagtgcctgc agaccatcag
     1081 ctgacaagct ttttacaccg cgcagacgcg gcccgggctg gcgctggaac cgaaagggat
     1141 gaagtttgca tctaacggag caagaatgga tgctgcgggt cgaaggcctg aagaaggtct
     1201 tccggtccgg cgaatcggac ctggtgctct ttgagaatct gtcgtttgag gtgaagcggg
     1261 gggaaatgct cgctatcgtg ggcgagtccg gagccgggaa gagcaccctg ttacacatcc
     1321 tgagcgctct tgaacgtgct tctgcaggtg acgtatactg cgcccaattg cggctgaatt
     1381 cgctgtcgca gaacgaagct tcggatttcc gcaaccgcga aaccggcttc gtatggcagt
     1441 ttcactatct gctgccggag tttacggcgg cagaaaacgt tgccatgccg ctgctgcttc
     1501 gggggcagac atggcagcag gcagaaccgg aagccttacg ctggttgcgt gaggtcggac
     1561 tggagcagcg ggcgcatcac cgctctggag agctttcggg cggcgagcag caacgcgtag
     1621 ctctggcccg cgcgctcatc acccggccca agctgttgct tgccgatgag cccacgggag
     1681 acctcgacgg aaagacggcg gaagcggttt ttgaactaat tgcgcgcttg cacagcgagc
     1741 accaactcac ctccctcatt gctacacaca atttgtcgtt tgcgcgccgc tgtcatcgcg
     1801 ttttgaggtt gcagcgcggt tcaatcgaag agatcacgcc ggattcgtta ccggcgtaag
     1861 gttttgttgc agggcaaaaa tcgtaagagc aagatttgta agacaaagtg caagaaactg
     1921 gtttggactt taacggaatg gcgcaggttt ccattccttc ggtaacgtga ttaaagagtt
     1981 gcagggcaat ttcggcgctt gggtttctcg gcactttgat tgcataatgg taagaagttg
     2041 ctaagaggca cagccaggcg tttcgggttc agtggtcg
//
LOCUS       DASPUL010000425         3966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_628249, whole genome shotgun sequence.
ACCESSION   DASPUL010000425 DASPUL010000000
VERSION     DASPUL010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628249"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VEJ00_10215"
     CDS             complement(<1..510)
                     /locus_tag="VEJ00_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXX01578.1"
                     /translation="MPTSVKVGEAETTFPQQSTAPVRILAADDQEHILEALDFLLQPQ
                     GYRVDKVRSPALVRDALASSSYDAILIDLNYTRDTTSGQEGLDLLSEIVALDATVPVI
                     VMTAWANVELAVEAMRRGARDFVQKPWENERLVSILRTQVELHRALQQAARLEAENRL
                     LRAEGRPEFL"
     gene            complement(516..2426)
                     /locus_tag="VEJ00_10220"
     CDS             complement(516..2426)
                     /locus_tag="VEJ00_10220"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXX01579.1"
                     /translation="MAIPAVGGVALAASVAAQTSGTTGLQTTPPAIRLQFEMPHSYNP
                     VNAYAPDRVPEPTLTNSPRIDQLIRDGKLYLSLKDAIDLALEDNLDLAIARYNLPIAN
                     TDILRTQAGGVFRGVNAGVVQGTPGGGVGGFGTGAPGGGAGGTTGGAGGAGAGASGLV
                     QSTLGAGTPVNSYDPAIIGTVGAEHQTTPLANETIYGVPLLQLNTGQLNIGYSQSFAS
                     GTTVSFAFSNNRQTTNSPFVNLSPALGSTFRFTFQQELLAGFGFGPNLRYLRIAKNDK
                     KISDIAFQDQVVATVTQIENIYWDLVNAYEQTQVNEQSYAFAEQTLENTKKQLELESV
                     PAMDVLRAEAEVSKRDQDLTVARTNLQLQATLMKNAITKSLDDPVLESIPVVPTDQMQ
                     AVSPQASQPIQDLISEAQSKRPELAESEVDLINRQISRKAAANALLPTVSLVGFYGGT
                     GLAGPLNPAFNEGPGITNSSTVPTDFAGALQNAFNNTAPDYYIGLNLNIPIRNRVAKA
                     DQYRSELEYRQAELRREQLRKQIRIEVRNAQYALDQSGARVEAARKARDLAQRTFDIT
                     KKEQDLGAGSSYQTLSAQRDLALAELDLVVAQTTYAKAKVELDRATGTTLEHNGILMQ
                     DAISGTVSRQTP"
     gene            complement(2453..3163)
                     /locus_tag="VEJ00_10225"
     CDS             complement(2453..3163)
                     /locus_tag="VEJ00_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXX01580.1"
                     /translation="MIRLRHLERSYKTPAGQTWVLRRINLEIREGEFITIMGPSGAGK
                     SSLLNALALLDDGWEGEYWFRDQAVHDMKRKQRGDLAKRNVGMVFQSYHLLDDLTVQE
                     NIDLPLSYKDIPRRERQGLVADTLDRFQIVAKKDLFPSQLSGGQQQLVGIARAVIHRP
                     ALLLADEPTGNLHSGQAKEIMELFRDLNQKGTTIVQVTHSETNAAYGSRTIQLHDGWI
                     VADTANAALTQPAEVSAS"
     gene            complement(3199..>3966)
                     /locus_tag="VEJ00_10230"
     CDS             complement(3199..>3966)
                     /locus_tag="VEJ00_10230"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXX01581.1"
                     /translation="PGSAPVSIVSQDTARKFWGQADPIGRTFYRAADPKTVFTVVGVV
                     GDVRSSTLNQQSPALYYPIAWRIGRAASRVTVVVMDVVARTDGPPEALASTLRQKVHE
                     LDPLLPLANVRTMQEWVANSAAQPRLNARLLAVFAGVALLIAAIGIYAVLAYSVTQRT
                     REIGLRMALGAMPGSVLRLVVGEGMLVGMMGVGIGLLGALVLARVMASLVYGIPVRDP
                     ATFAGVAVVLAVVALAACYIPGRRAAKVDPVVALRYE"
BASE COUNT          714 a         1271 c         1179 g          802 t
ORIGIN      
        1 caggaattca ggacggccct cggcgcggag caggcgattc tccgcttcca gcctcgcagc
       61 ctgctgcaga gcccgatgca gttcgacctg cgtgcggaga attgagacga gccgctcatt
      121 ctcccagggc ttctgaacga aatccctggc gcctcgccgc atggcctcaa cggcgagctc
      181 tacattcgcc cacgccgtca tcacgatcac cgggacggtc gcgtccaggg ctacgatctc
      241 agagagcaga tcgaggccct cctgccccga agtcgtgtcc cgtgtgtagt tcaggtcgat
      301 gagaattgcg tcgtacgagc tgctggcgag cgcatcccgc accaaggcag gcgaacgaac
      361 tttgtcaaca cggtagccct gcggctggag cagaaagtcc agcgcttcca ggatgtgttc
      421 ctgatcgtcc gcggccagaa tccgaaccgg cgcggtggac tgctgcggga acgtggtttc
      481 cgcttcgccg actttgactg aggtgggcat gcttgctaag gcgtttggcg agacaccgtc
      541 ccgctgatcg cgtcctgcat cagtatgcca ttatgttcca acgtggtgcc ggtggcgcga
      601 tccagttcca cctttgcctt tgcgtaagta gtctgggcca caacaagatc cagttcggcc
      661 agcgccagat cgcgctgcgc cgagagcgtc tgatagctcg acccggctcc caggtcctgc
      721 tccttcttcg ttatgtcgaa ggtgcgctgt gccaggtcgc gtgccttacg cgcagcctcc
      781 acacgtgcgc cggactggtc gagggcgtac tgggcgtttc gcacttcgat gcgaatctgc
      841 ttccgcaatt gctcgcgccg cagttccgcc tgccggtact ccagttcgga gcgatactgg
      901 tccgctttcg ccactcgatt gcgaatcggg atattcagat tcaagccgat gtagtagtcg
      961 ggcgcggtgt tgttgaacgc attctgcagc gcaccggcga agtccgtggg aacggtggaa
     1021 ctgttcgtga tgcctggccc ttcgttgaag gcgggattca gcggcccggc cagccccgta
     1081 cctccataga agccaaccag cgagacggtg ggcaacaagg cattcgccgc agccttccgg
     1141 ctgatttggc ggttaatgag gtcgacttcc gactcggcca gttcgggccg cttgctctgc
     1201 gcctcggaga tcaggtcctg gatcggctgg ctcgcctgcg gcgagaccgc ttgcatctgg
     1261 tcggtcggaa ccactggaat cgactccagc accggatcgt ccaggctctt ggtgatcgcg
     1321 ttcttcatta atgtcgcttg caattgcaga ttcgtgcggg ccaccgtcag gtcctggtcg
     1381 cgcttggaaa cttcagcctc cgcacggagt acgtccatcg caggaacgct ttccagttcc
     1441 agctgcttct tcgtgttttc cagcgtctgc tctgcgaatg cgtaggattg ctcattgact
     1501 tgcgtctgct cataagcatt caccaggtcc cagtagatgt tctcgatttg cgtaactgtg
     1561 gcgaccacct ggtcctgaaa ggcgatgtcg gaaatctttt tgtcgttctt ggcgatccgg
     1621 agatagcgga ggtttgggcc aaagccgaag cccgccaaaa gctcctgctg gaatgtgaaa
     1681 cgaaacgtcg accccagagc cggcgacaaa tttacaaagg ggctgttggt tgtttgccgg
     1741 ttgttgctga acgcgaaaga gacggtcgtg ccgctggcaa aggactggct gtacccgatg
     1801 ttgagctgtc cggtgttgag ctgcaacaac gggactccgt agatcgtctc gttggcgagc
     1861 ggcgtcgtct ggtgctctgc cccgaccgtg ccaatgatcg cgggatcgta agaattgact
     1921 ggcgtgcccg cgcccagtgt ggactgcaca agtcccgaag cgcctgctcc ggctcccccc
     1981 gcgccgcccg tagttcctcc ggctcctccg cccggcgccc ccgtgccaaa tcctcccacg
     2041 ccgcctccgg gagtgccctg aaccaccccg gcattgactc cgcgaaacac gccgccagcc
     2101 tgcgtgcgca ggatatcggt attcgcgatc ggtaggttgt aacgcgcgat agccaggtcg
     2161 agattgtcct cgagcgccag atcgatagcg tctttcagcg acaagtagag tttgccgtcg
     2221 cgaatcagct ggtcaatccg gggcgagttg gtcaacgtcg gctcgggaac cctgtcgggg
     2281 gcgtaagcgt tgaccgggtt ataggagtgc ggcatctcaa actggaggcg gatcgcgggc
     2341 ggcgtcgtct gtagtcctgt tgtgcccgag gtctgcgccg ccacactggc ggccagagca
     2401 acgccgccga cggcggggat cgccaatctc aagattctcg taacgaatga gctcacgatg
     2461 cgctgacctc cgctggttgt gtgagcgcgg cattcgccgt atccgccacg atccatccgt
     2521 cgtgcagctg aatcgtgcgg ctgccgtagg cggcattcgt ctccgagtgc gtcacttgca
     2581 caatcgtcgt tcctttctgg ttcaggtcgc gaaagagttc catgatctcc ttggcttggc
     2641 cggagtgaag attccccgtg ggttcgtccg cgagcagcaa ggccggccgg tgaatcacgg
     2701 cgcgcgcgat tcccaccaac tgctgctgcc cgccagaaag ctggctggga aacaaatcct
     2761 tcttggccac gatctggaag cgatcgagcg tatcggccac caggccctgc cgctcgcgcc
     2821 gcggaatgtc cttgtaggaa agtggcaggt cgatgttctc ctgcacggtg agatcgtcga
     2881 gcaggtgata actctgaaaa accatcccga cattccgctt cgccaggtcg ccgcgctgct
     2941 tgcgcttcat gtcgtgcacc gcctggtcac ggaaccagta ttcgccctcc cagccgtcat
     3001 ccagcagcgc gagagcgttc agcaaagacg acttccccgc ccccgagggc cccatgatgg
     3061 tgatgaattc gccttcacga atttcgagat tgatgcgacg cagcacccac gtttgcccgg
     3121 ccggagtctt gtaacttcgt tccagatgcc ttaaacggat catctcaatc tccaatctct
     3181 atctcgattc ctaggcgtct actcgtaccg cagcgccact accgggtcga ccttggcggc
     3241 gcggcgccct ggaatgtagc aggccgcaag cgctaccact gccagcacaa ccgcaacgcc
     3301 tgcgaaggtt gccggatcgc gcacagggat gccataaacc aggctggcca tgacccgcgc
     3361 cagcaccagg gcgcctagca gcccgatgcc gacccccatc atcccaacca acatgccttc
     3421 gcccaccacc aaccgaagca cgctgcccgg catggcgccc agcgccatgc gcaagccgat
     3481 ctcgcgcgta cgctgcgtaa ctgaataggc aagcacagcg tagattccaa tggccgcgat
     3541 cagcagagca acgccggcaa acaccgctag cagccgggca ttgaggcggg gctgggctgc
     3601 gctgttcgcc acccactcct gcatggtccg cacgttggcc agtggaagca gcgggtccag
     3661 ctcgtgtact ttctgccgca gcgtggaagc gagcgcttcg ggtggaccgt cggtgcgcgc
     3721 gacaacgtcc atcaccacga ccgtaaccct cgacgctgcc cggccgatcc tccacgcaat
     3781 cggatagtaa agcgcgggcg attgctggtt cagcgtgctg ctgcgcacat cgcctaccac
     3841 ccccacaacc gtgaaaacag tcttggggtc cgccgccctg tagaatgtcc gcccgatcgg
     3901 gtcggcctgt ccccagaatt ttcttgccgt atcctggctg acgatcgaca ctggcgccga
     3961 ccccgg
//
LOCUS       DASPUL010000426         3551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1854113, whole genome shotgun sequence.
ACCESSION   DASPUL010000426 DASPUL010000000
VERSION     DASPUL010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854113"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VEJ00_10235"
     CDS             <1..291
                     /locus_tag="VEJ00_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01582.1"
                     /translation="PAASPAPQTPELTLRRVNNDANMLYQAVWLADAEPTSSQMEALA
                     RTEKDVASLLQRWNEFKQSDLPALNRALRESKAPEIQLEPEPHHEEPLVDEE"
     gene            complement(460..2994)
                     /locus_tag="VEJ00_10240"
     CDS             complement(460..2994)
                     /locus_tag="VEJ00_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXX01583.1"
                     /translation="MAESLAEFFVENFQARRNECAYRQRRGYRTESFTYGEVLQLACG
                     FARTLAARGVGKGDRVMLWGRNCAEWVAVYFGCAMSGVIVVPMDDGASADFALRVYRQ
                     VQVKMLVGSRRHVQECATARGAIATLVLEDLPRSTDASEVFPGGASPSRDDVLQLVFT
                     SGTTAEPRGVVITHGNVLANIAPFQQEMRPYLKYEKLVHPLRFLNLLPLSHVFGQFLG
                     MFLPPLLGGTVIFQDELKPSEVVRTLRRERVSVLVSVPRVLQSLKQKVEHDLENRGET
                     AAFRRRFQAAEAKHFLHRWWIFRAIRRQFGWKFWAFISGGAALDRETEEFWGRLGYAV
                     IQGYGLTETTSLISVNHPFRLGKGSIGKVLPGREVKLAEDGEILVRGGGVASGYLEAQ
                     GPQRVSDEQGWYHTGDIGALDEAGNLYFKGRKKEVIVTPAGLKVYPDDLEAALRQQPE
                     VKDCVVVGVDRGGNAEPCAVVILRDGAAAEPVVQRANRSLADYQRIRMWLEWPQEDFP
                     RTNTQKPRRNVIRDVAQAYILQTSARVDDQERPMAELIARVAGRPVTGLHEHTSLDSD
                     LGLSSLDRVELLSALEDRYQVDLSETRFSAVKTVGDLERMLRGEAAPGVGYHYPRWAL
                     RWPVTWLRLLTHYLLLRPAIVLLGWPRIEGREHLRGVKGPLLVVSNHVGDVDPGFILT
                     ALPARFRHRLAVATGGEALEALRTPPSNRNFLLRIYDRVQWLLGVSLLNLFPLPREAG
                     FRRSFAYAGEAVDRGYSVLVFPEGRHTNDGKINPFRAGIGLLAGNLGIPVLPMRIIGL
                     FELKEAGKKFAPPWKIGVRIGEPFRFARDADPEKIAAELQQAVERL"
     gene            3190..>3551
                     /locus_tag="VEJ00_10245"
     CDS             3190..>3551
                     /locus_tag="VEJ00_10245"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXX01584.1"
                     /translation="MPLLLCLLAAFPLLGRAQDRDKSDAAAVRSLELKWTESYKQRNI
                     DILSSLLAEDFVITIEDGSVYSKAGYISHSADASTRVDVAELSDLKVRVHGPVAVVTG
                     AYHEKGESNGRPYEYHDRL"
BASE COUNT          750 a         1173 c          981 g          647 t
ORIGIN      
        1 ccggcggctt cccccgctcc gcaaactccc gaactgacgt tgcgccgtgt caacaacgac
       61 gccaatatgc tgtaccaggc cgtctggctc gccgacgcgg aaccgacgtc gtcgcagatg
      121 gaagccctgg cgagaacgga gaaggacgtc gccagcctcc tgcaacgttg gaacgaattc
      181 aagcaatcgg acttgccggc attgaacaga gcgctgcgcg aatcgaaagc ccccgaaatt
      241 cagctggagc cggagccgca ccacgaagaa ccgctagtgg acgaagagta gcccctgggc
      301 ggagcatgtt tcgcagatgc gaccagctag tatcctgagg cgcggttttt cggcgtcgaa
      361 ggacctgtgc gttggctggc agcgttgaga tcgtgaacga gtgcataggt ccttcgccgc
      421 aaacagcgcc gctcaggatt ggaatttagc gcgagttcct tacagccgtt ccactgcctg
      481 ttgtagttct gctgcgatct tctcgggatc tgcatcgcgc gcgaatctga atggctctcc
      541 aatgcgaacc ccaatcttcc acggtggcgc aaatttctta ccggcctcct tcagctcaaa
      601 caatccgata atccgcatcg gcagcaccgg aattccgaga ttccccgcga gcagcccgat
      661 ccccgcgcga aagggattga tcttgccatc gttggtgtgg cgaccttcag gaaacaccaa
      721 cacgctgtag ccgcgatcga ccgcctcgcc cgcgtaggcg aagctcctcc ggaagccggc
      781 ttccctcggc aagggaaaca gattgagcag cgagactccc agcagccact gcacgcggtc
      841 atagattcgc agcaagaagt ttcggttcga aggcggagtc cgcagcgctt ccagggcttc
      901 gccgcccgtg gcgaccgcca gacgatggcg gaaacgcgcc ggcagggcgg tgaggatgaa
      961 tccgggatcg acatcgccca cgtgattcga aacaaccagc aacggaccct tcaccccgcg
     1021 cagatgctcg cgcccctcga tgcgcggcca ccccagcagc acgattgccg gccgcagcag
     1081 cagatagtga gtgagaagcc gcagccatgt caccggccag cgcaacgccc agcgcggata
     1141 gtgatacccc acgccgggcg ccgcttcgcc gcgcagcatg cgttccagat cgccaaccgt
     1201 cttcacggcg ctgaagcgag tctcgctcag gtctacctga tagcgatctt cgagagcgct
     1261 caacagctcg actcgatcca gcgaactcag gcccagatca gaatccaggc tcgtgtgctc
     1321 gtgaaggccg gtcacaggac gaccggcgac ccgcgcgatt aactctgcca taggtctttc
     1381 ctgatcatca acgcgggcgc ttgtttggag tatgtatgcc tgcgcgacat cccggatcac
     1441 gttgcgccgc ggcttctgcg tattcgtgcg cggaaagtcc tcctgcggcc actcgagcca
     1501 catgcgtatg cgctggtagt ccgccagcga tcgattggcg cgctgcacca caggctccgc
     1561 cgcagcccca tcgcgcaaaa tcacgaccgc acatggctcg gcgttgcctc cccgatccac
     1621 gccgacaacc acgcaatctt tcacttcagg ctgctggcgc aacgccgctt cgagatcatc
     1681 gggatatacc ttcagcccgg ccggcgttac gatcacctct ttcttgcggc ccttgaaata
     1741 aagattgccc gcttcatcga gggcgccgat atcgccggtg tgataccagc cttgctcgtc
     1801 cgagactctt tgcggccctt gcgcctccaa ataccccgag gccacacctc ctccccgcac
     1861 cagaatttcg ccatcctctg ccagctttac ctcccggccc ggcaacacct tcccgatcga
     1921 gcctttgccc aggcggaacg gatgatttac gctgatcagc gaagtcgttt cggtcaaccc
     1981 atatccctga atcacggcgt agcccagccg tccccagaac tcttcggttt cacggtcgag
     2041 cgccgcacct cccgagatga atgcccagaa cttccatcca aactgccgac gaatagcgcg
     2101 aaagatccac cagcggtgca gaaagtgctt cgcctcagct gcctggaatc gtctgcggaa
     2161 cgcggcagtt tctcctcgat tctccagatc gtgctcgact ttctgtttca aggattgcag
     2221 cactcgaggg acactcacca gcaccgagac gcgctcgcgg cggagggtgc gcactacttc
     2281 cgaaggtttg agttcatcct gaaaaatcac cgtcccgccg agcagaggag gcaggaacat
     2341 tccgaggaac tgtccaaata cgtggctcag cggcagcaga ttcaggaagc gcagcggatg
     2401 cacgagcttt tcatacttca agtacggtcg catctcctgc tggaatggcg cgatgttagc
     2461 cagaacattg ccatgcgtga tcaccacgcc ccggggctct gctgttgtgc ccgaagtaaa
     2521 gacaagctgc aagacatcgt cgcgcgaagg agaagcaccg cccgggaata cctcactggc
     2581 atcagtagag cggggcagat cctcgagaac cagcgtagct attgcacccc gagctgtggc
     2641 gcattcctgc acgtgccgac gcgatcccac gagcatcttc acctgcacct gccgatacac
     2701 gcgcagggca aagtccgcag acgctccgtc gtccattgga accacaatca cgccggacat
     2761 cgcgcacccg aagtacacag caacccactc cgcacagttc ctgccccaca gcatcacgcg
     2821 atcgcctttg cccaccccgc gtgccgcgag ggtgcgggca aagccgcagg ccaactgcag
     2881 aacctcgcca taagtgaacg attcggtccg gtagccgcgc cgctgccggt aggcgcactc
     2941 gttgcggcgc gcctgaaagt tctctacgaa gaattctgct aacgattcgg ccatagcccc
     3001 gacgcgattt gcgaatcgca ccgctctcta cccaagtcta gtagaacaac ggcggggaag
     3061 cgccagactg agatacagtt cccttactgc aacatccgca acccggaatt acggatgcgc
     3121 ggattccaga aggaccttgc cgcaggcggc tgactgtgcg acatagtaga gatgatttct
     3181 cgccggaaca tgcctttgct cctctgcctg ctcgctgctt ttcccttgct tggccgggcc
     3241 caggatagag acaagtcaga tgcggccgcg gtgcgctctc tcgagctgaa gtggaccgaa
     3301 tcctacaagc agcgcaacat tgatattctc tcttcgctgt tggccgagga cttcgtcatc
     3361 accatcgagg atgggagcgt gtatagcaaa gccggctaca tcagccacag cgccgacgct
     3421 tctacgcgcg tcgacgtggc cgaactgtcc gatctgaagg tgcgagtaca cggcccggtc
     3481 gcggtcgtaa ccggggcata ccacgagaag ggtgaatcca acgggaggcc ctacgaatat
     3541 cacgaccgcc t
//
LOCUS       DASPUL010000427         4427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1578490, whole genome shotgun sequence.
ACCESSION   DASPUL010000427 DASPUL010000000
VERSION     DASPUL010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1578490"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /gene="ilvD"
                     /locus_tag="VEJ00_10250"
     CDS             <1..1540
                     /gene="ilvD"
                     /locus_tag="VEJ00_10250"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXX01585.1"
                     /translation="ETMPCNFHLRRLSAKVKEGVRAAGGTPMEFNTIAISDGETMGTE
                     GMRASLVSRELIADSIELVCRGQMFDAVVCVVGCDKTIPAAAMALARMNLPGMVLYGG
                     TIAPGNYRGRDVTIQDVFEAVGANAAGRITDKELHDLEDVACPGAGACGGQYTANTMS
                     TVMEMIGLSPMGFNSVPAVDAEKDQVAFDCGRVVMNVLRQGLKPRDILTRPAFENAIA
                     SVAATGGSTNAVLHLLAIAREAGVRLEIDDFQTVSQRTPLLADLKPSGRFVASDMHRA
                     GGIRLLAKRLLDGKYLHPAATTVTGRTISAEAEGAAEASGQEVIAPLNKPLKTTGGLV
                     ILKGNLAPEGCVAKISGHERLEHRGPARVFDSEEDAMTAVTSKKIRAGDVVVIRNEGP
                     KGGPGMREMLGVTAAIVGEGLGESVALLTDGRFSGATHGLMAGHVSPEAALGGPVAGV
                     RDGDVIRFDVRQRILEVEIGEDVLRQRMAEWKPPQPRYAKGVFAKYAALVSSASQGAV
                     TGPR"
     gene            1614..3023
                     /gene="leuC"
                     /locus_tag="VEJ00_10255"
     CDS             1614..3023
                     /gene="leuC"
                     /locus_tag="VEJ00_10255"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXX01586.1"
                     /translation="MTRARTLFDKIWDAHLVAEPAGRSPILYIDLHLVHEVTSPQAFD
                     GLRAAGRKVRQPLRTVATVDHNVPTEPRGTPITDLIAAAQIAALQRNCKEFGIRLFDI
                     DSDEQGIVHVIGPELGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQCL
                     AQQKPKTMKIDVHGHLAEGVTAKDLALGIIGQIGTDGATGHVIEYAGPAVRALSMEGR
                     MTLCNMSIEAGARAGMVAPDETTIAYVNDRRFAPRDAEWEAAVAHWQSLPTDEGATFD
                     RVVEIDAAQLCPFVTWGTNPGMVAPVTGRVPDIKGFQDGDRRAAELALQYMGLEPGRR
                     IEDIEVDRVFIGSCTNSRLEDLRAAARVAKGYRVSPKVRAMVVPGSQAVKRAAEHEGL
                     NKIFLSAGFEWRESGCSMCLGMNPDILQPGERCASTSNRNFEGRQGRGGRTHLVSPMM
                     AAAAAIAGHFTDIRNWKFR"
     gene            3039..3650
                     /gene="leuD"
                     /locus_tag="VEJ00_10260"
     CDS             3039..3650
                     /gene="leuD"
                     /locus_tag="VEJ00_10260"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXX01587.1"
                     /translation="MKPFTVHRGRVAPLDRVNIDTDQIIPKQFLKRIERTGFGQYMFY
                     DWRFGTDGKKNPDFVLHDPRYEGATVLVAGKNFGCGSSREHAVWALADFGFRAVISSS
                     FADIFANNSAKNGFLTVRLKEDEAAEIMRRAQGIDDYQVTVDLERCEVCDDQGFKAKF
                     PVDDFVRHCLLNGLDDIGLTLQHEAEIAAYEKEHPAPAGLKVS"
     gene            4000..>4427
                     /locus_tag="VEJ00_10265"
     CDS             4000..>4427
                     /locus_tag="VEJ00_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HXX01588.1"
                     /translation="MPRLMALLGIGTLCVSLFACSGASDAKQQKAQAAGPRDVSVAVA
                     TVQRQDVPVYLTGLGTVTAFNTANIKSRVDGQIMKVNFQEGQFVHEGELLIEIDSRPY
                     QVQLEQMQAQLFRDQAQLRDAKLNLDRYTALIPSGSIAQQ"
BASE COUNT          903 a         1172 c         1484 g          868 t
ORIGIN      
        1 cgaaaccatg ccttgcaatt ttcacttgcg ccggctctcg gccaaggtga aagaaggcgt
       61 tcgcgccgcc ggcggcacgc ccatggagtt caataccatc gccatctccg atggcgagac
      121 catgggcacc gagggtatgc gggcgtcgct ggtgagccgg gagttgatcg ccgattcgat
      181 tgaactggtc tgccgggggc agatgttcga tgccgtggta tgcgtggtgg gctgcgacaa
      241 gacgattcca gcggcagcga tggccctggc gcggatgaat ctgccgggaa tggtgctcta
      301 cggcggcacg atcgcgccgg ggaactatcg cgggagagac gtcacgattc aggatgtgtt
      361 tgaggcggtc ggggcgaatg ccgcgggcag gattacggac aaagagctgc acgatctgga
      421 agatgtcgcg tgcccgggag cgggcgcctg cggggggcaa tatacggcga acacgatgtc
      481 gaccgtgatg gagatgatcg gactttcgcc aatgggcttc aacagcgtac ccgcagtgga
      541 cgcggagaaa gaccaggttg ccttcgattg cggccgagtt gtgatgaatg tcctgcggca
      601 aggactgaaa cctcgcgaca ttttgacgcg gcctgcgttt gagaatgcca tcgcgtccgt
      661 cgctgctact ggaggatcca ccaacgccgt gctgcacttg ttggctatcg cgcgagaagc
      721 cggcgtccgg cttgagattg acgacttcca aaccgtcagc cagcgcactc ctctgctcgc
      781 cgatcttaag ccttcgggcc gtttcgtcgc atccgatatg caccgtgccg gcggaatccg
      841 cctgctggcc aagcggctgc tggatgggaa gtacctgcat ccggcggcga ccactgtaac
      901 gggtcgcaca atttccgctg aggctgaagg cgcagctgaa gcttcggggc aagaggtcat
      961 cgccccactg aacaaacctc tgaagacgac gggcgggcta gtgatcctca agggaaatct
     1021 cgcaccggag ggctgcgtgg ccaagatcag cggtcacgag cggctggagc atcgcggccc
     1081 ggcgcgcgtg ttcgactccg aagaggacgc gatgactgcg gtcaccagca agaagattcg
     1141 cgcaggagac gtggtcgtca ttcgcaatga aggtccgaag ggcggccctg ggatgcgtga
     1201 gatgctcggc gtaaccgcgg cgattgtcgg cgagggactg ggggagtctg ttgctctttt
     1261 gaccgacggc cgcttcagcg gcgcaaccca tgggctaatg gccggtcacg tttctcccga
     1321 ggctgccttg ggggggccgg tcgccggcgt gcgcgatgga gatgtgatcc gcttcgacgt
     1381 gcgccagcgc attctggagg ttgaaatcgg cgaagatgtc ctgcgccagc ggatggccga
     1441 gtggaagccg ccgcagccgc gttatgctaa gggagtattt gccaagtacg cggcgctggt
     1501 ttcctcggcg tcgcagggcg cggttacggg tccaaggtag ggacaaagtc aaaatcccca
     1561 ccctaaccgc aaaaagcggc ggttaggatg gggcacccac acagttgaga ttcatgacga
     1621 gggcgagaac actcttcgac aagatctggg acgcgcacct ggtggctgag cccgccggac
     1681 ggtctccgat tctttatatc gacctgcatc ttgttcatga ggtgacctcg cctcaggcgt
     1741 ttgatggact gcgcgccgct ggaaggaaag tgcgacagcc gctgcggacg gtcgccaccg
     1801 tcgatcacaa cgttccgacc gagccgcggg gcacgcctat tacggacttg atcgccgcgg
     1861 cgcagatcgc ggctctgcag cgaaattgta aagagtttgg catccggctt ttcgatatcg
     1921 actcggacga acaaggaatc gtccatgtga ttggtccgga gttgggtttg acgcagcccg
     1981 gaatgaccat cgtctgcggc gacagtcata ccagcacgca tggagcgttc ggcgcgctgg
     2041 catttggtat cggaacgtcg gaagttgagc acgttctggc gacacagtgc ctggcgcagc
     2101 agaagccgaa gacgatgaag attgatgtgc atgggcatct ggccgaaggt gtgacggcga
     2161 aggacctcgc gctgggaatc atcgggcaga tcgggactga cggtgcaacc gggcacgtga
     2221 tcgagtacgc aggtccggct gtacgagcgc tctcgatgga aggccggatg acgctgtgca
     2281 atatgagcat cgaagccggg gcgcgggcgg gcatggtggc accggatgag acgaccattg
     2341 catatgtcaa cgacagacga ttcgctccgc gggatgcgga atgggaagcg gcggtggcgc
     2401 attggcaaag cctgccgacc gatgagggtg cgacattcga tagagtcgtg gagatcgacg
     2461 ccgctcagct ttgcccgttt gtgacctggg gaacgaatcc agggatggtg gcgccggtga
     2521 cggggcgcgt tcccgatatc aaaggtttcc aggatggcga ccggcgggca gcggagctcg
     2581 ctttacagta tatggggctc gagcccggtc gccggatcga agatatcgag gtcgaccgcg
     2641 tgttcatcgg atcttgcaca aactccaggc tggaagattt gcgggccgcg gcgcgcgtgg
     2701 ccaaggggta tcgcgtgagt ccaaaggttc gggcgatggt cgtgcccgga tcgcaagcgg
     2761 tgaagcgagc ggctgagcat gagggactaa acaagatttt tctgagtgcc gggtttgagt
     2821 ggcgcgagtc gggatgctcg atgtgccttg gtatgaaccc cgacattttg cagccgggcg
     2881 aacgctgcgc gtcgaccagc aaccgcaact tcgaaggacg gcagggacgc ggcgggcgca
     2941 cgcacctggt gagtccaatg atggcggcgg cggcggcgat tgccgggcat tttacggata
     3001 taagaaattg gaagtttcgg tgaagtaggg aatacgcaat gaagccattc actgtgcacc
     3061 gtggccgcgt agcgccgctt gatcgtgtca acatcgatac cgaccagatc atccccaagc
     3121 agtttctgaa gcgaatcgag cgcaccggct ttggccagta tatgttttac gactggcggt
     3181 ttggcacgga cgggaagaag aatccggatt tcgtgctaca tgacccacgc tacgaaggcg
     3241 caactgttct ggtggcgggg aagaactttg gatgcggttc gtcacgcgag cacgcggttt
     3301 gggccctggc tgattttggt tttcgagccg tgatctcgtc ctctttcgcc gacatttttg
     3361 ccaacaacag cgcgaaaaac ggctttctca ccgtgcgact caaggaagac gaggcagccg
     3421 agatcatgcg gcgggcgcaa gggattgacg actatcaggt tacggtcgac ctcgagcgat
     3481 gcgaggtttg tgacgatcaa ggattcaagg caaagtttcc ggtggacgac ttcgtccggc
     3541 attgcctgct gaacgggttg gacgacatcg gcctcacgtt gcagcacgag gcagagatcg
     3601 cggcctacga gaaagagcat ccggcgccgg ccggactcaa ggtttcctga caggtaacgg
     3661 aagttacggg caggttaagg taaagaattg taaaacgcgg gctccggcct tgacctcgtg
     3721 ccagccccca gttgatgctg gttttgtagc attctacgat tgtagctggc gctctggccc
     3781 tatctttaag ggcgacctcg cgggcaggct ccatcgcggc agagagattt ttctgcctct
     3841 gtaaacgcag tgctgagacc gagcctaaga aattacgtct aataagtggg acttgtcgtt
     3901 gcggtgcgaa taagcggctt tggattcatt cgtgagactg atccggtgaa tatgagcgat
     3961 cgaatttccc gtcggcagag ggttcggggc catgatcttg tgcctcgttt gatggcgctt
     4021 ttgggcattg gaacattgtg tgtcagcctg ttcgcctgca gcggcgcgtc ggatgccaag
     4081 cagcaaaaag cgcaagctgc aggaccgcgc gacgtctcgg tggcggtagc aactgtgcaa
     4141 aggcaggatg tcccggttta cctcaccggt ttgggaactg tgactgcctt caacacagcc
     4201 aacatcaaaa gccgcgtcga tgggcagatc atgaaggtca acttccagga agggcagttc
     4261 gtccatgaag gagagctgct gattgaaatt gactcccgtc cgtaccaggt gcagctcgag
     4321 cagatgcagg cgcagttgtt ccgcgaccag gctcaattgc gggacgccaa gctcaatctg
     4381 gatcgctata ctgcgctgat tccttcgggg agcatcgcgc aacagca
//
LOCUS       DASPUL010000428         5328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_220863, whole genome shotgun sequence.
ACCESSION   DASPUL010000428 DASPUL010000000
VERSION     DASPUL010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220863"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /locus_tag="VEJ00_10270"
     CDS             <1..1731
                     /locus_tag="VEJ00_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXX01589.1"
                     /translation="GAVLTIDLTNRCNMMCDPCFMDANQVGFVHELSWEEIKTMLDNA
                     ITIKPRRQMSVQFSGGEPTLSPYFLDAVRYARKVGYNSVQAATNGIEFAKSFEFAEQA
                     VEAGMRYAYLQFDGIGNAANAHRMVGNLFDVKLKAIENLHKAGCEIVPVITIVNGINN
                     EQVGRIIKFALDNPKTISFLSFQPVSFTGRDEAVTDERRQAQRYTLSHLAHDVKNQTG
                     LGEPARDWFPISFMGTFSDWADLMHAEDSNNNWGQLSCGCHPNCGTGMAVMIDKETKE
                     AVPVTAFLHGDQLAKDIAKVNDAARGKFLTVFGMALCLMKNYDPFQSPTHFKLVDLLR
                     KFDKNYNATGHNYGKVGPDRTLDDVMKRRTGDRWNFLFIAGMWFQDLFNYDFRRTEQC
                     IIPYATQEGEISFCAYNTGVGWRNIIEKMHMTATLTKWYEEHGRHEIFAGGKKVKMDE
                     QHVDYLKLREEIVTNELQHDLDKLGIAKNAREEKIRARDAKQKASGSGLPASGGEARG
                     PQKNDEAYNARMASLYREVVLKEKPVQSQDGFIPLGALVTPALAAKTNGNGNGARAPK
                     PEMAEPVAGD"
     gene            2169..3254
                     /locus_tag="VEJ00_10275"
     CDS             2169..3254
                     /locus_tag="VEJ00_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HXX01590.1"
                     /translation="MPRRVLVLTAGLGFALLFLSSAAMAQYKLRNLDSNQVNGAGHDD
                     PLIVNAWGIVHGPGTPWWVSDNTSGWSTLYDGTGKAQPLRVLIPTAGNGPASPSGNNG
                     PGSPTGVVFNPTAFTKAAEFQVGRWPSLFIFATLDGTISGWTFKTNSNQAVIGWDNSS
                     KGSSYTGLAITNRASGNLLFAADNVNGKVDVYDATFTPVNMPGAFSDSVAIPAGFAPF
                     GIRDIDGIVYVMFASTTGGKGGFVEQFKEDGTPVTPGKALISGTPLNQPWGIAVAPKN
                     FGVLSNTLLISNNTNFGTINGFNPVTGQFVGTMRDFATGKPIVIDQLWGIDFGDGLGG
                     NGPASELYFAAGPSNNLAGTFGSIVVK"
     gene            3674..>5328
                     /locus_tag="VEJ00_10280"
     CDS             3674..>5328
                     /locus_tag="VEJ00_10280"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXX01591.1"
                     /translation="MTSVLRSVLVRGCGAVLALALMCGLSTLLRAQDVASLTGVVTDA
                     TGAVVPDVDVKLLDTKTNATFETKTNSLGAYQFVKLLPGPGYKLTFSKQGFSTLTIPD
                     IYLATGNSHTQDAHLAVGALSETVEVKGEGGAVSLDTTDTAVSTDFDMTLTHELPVQL
                     RDNPAALMTLQPGVVSVGAGSGSDTNFTRAGAVTGARDDQNNVTVDGLDVNDFGTGQA
                     FVVNGNAPVDSIQEFRGESANPLSAEGRGSGAQIALTTKSGTNTWHGAADEYNRVRAY
                     AANRYFNNLNGLPRTPLIRNQFGAEVGGPVLKDKLFFFFDYFGRRDNQGVSVENIVPL
                     DTWRGDGTSSTPGIPQVGYINNGAGCTNASRANTTPGCISYIPNTAASGQTLTGLDPQ
                     GTGSSSALLAFINGRYPHANDLGVGDGINTGGFRFNTPAHLTENAYIGRIDYNLSSKM
                     RLFGRFTILRQNTDDDVNFPAPVQFPGDPLTHAIIDTSYAYVIGHTWTISNTKVNQFV
                     YGETKQQLNFPTLFNPTGFIQNQNFGNVTAPFSNPASQGRTVP"
BASE COUNT         1189 a         1501 c         1605 g         1033 t
ORIGIN      
        1 ggtgctgtgc tcaccatcga tctgaccaac cgctgcaaca tgatgtgcga tccctgcttc
       61 atggacgcga accaggtcgg cttcgtgcac gagttgagct gggaagaaat caagaccatg
      121 ctcgacaacg ccatcaccat caagccgcgc cgccagatga gcgtgcagtt ctcgggcggc
      181 gagccgacgc tgtcgccgta cttcctcgac gccgtgcgct acgcgcgcaa ggtgggatac
      241 aactcggtgc aggcggcgac caacggcatc gagttcgcga agagttttga attcgcggag
      301 caggcggtgg aagccggcat gcgctacgcg tatctgcagt tcgatggcat cggcaacgcg
      361 gccaatgcgc accgcatggt cggcaacctg ttcgacgtga aactgaaggc gatcgagaac
      421 ctgcacaagg caggatgcga gatcgtcccg gtcatcacga tcgtgaacgg catcaacaac
      481 gaacaggtgg ggcgcatcat caagttcgcg ctcgacaatc cgaagacgat cagctttttg
      541 tcgttccagc cggtgagctt taccggccgc gacgaagcgg tcaccgacga gcgtcgtcag
      601 gcgcagcgct acacgctgtc gcacttggcg cacgacgtga aaaatcagac cggccttggc
      661 gagcccgcgc gcgactggtt cccgatctcg ttcatgggaa ccttcagcga ttgggctgac
      721 ctgatgcacg ccgaggactc caacaacaac tggggccaat taagttgcgg ttgtcacccg
      781 aattgcggga cgggcatggc ggtgatgatc gacaaggaaa cgaaggaagc ggtgccggtt
      841 accgcgtttc tgcacgggga tcagctggcc aaggatatcg ccaaagtgaa cgacgcggcg
      901 cgcggaaagt ttctgaccgt gttcggcatg gcgctgtgcc tgatgaagaa ttacgatccg
      961 ttccagtcgc cgacgcactt caagctcgtc gatctgctgc gcaagttcga taagaactac
     1021 aacgccacgg gccacaacta cggcaaagtc ggtccggacc gcacgctcga tgacgtgatg
     1081 aagcggcgca cgggcgaccg ctggaacttc ctgttcatcg cgggcatgtg gttccaggac
     1141 ctgttcaact acgacttccg gcggacggag cagtgcatta ttccctatgc gacgcaggag
     1201 ggcgagattt cgttctgcgc gtacaacacg ggcgtgggct ggcggaacat catcgagaag
     1261 atgcacatga ccgcgacgct gaccaagtgg tacgaggagc atgggcggca cgagattttt
     1321 gccggcggca agaaagtgaa gatggatgag cagcacgtcg actatctcaa gctgcgggaa
     1381 gaaatcgtga ccaacgagct gcagcacgat ctcgacaagc tgggcatcgc caagaacgcg
     1441 cgcgaggaga agattcgggc gcgggatgcc aagcagaagg cgtccggctc cgggcttccg
     1501 gcttccggcg gcgaagcgcg tggtccgcag aagaatgacg aggcttataa cgcgcgcatg
     1561 gcgtcgctgt atcgggaagt ggtgctgaaa gagaagccgg tgcagtcgca ggacgggttc
     1621 attccgctgg gcgcgctagt gacgcctgcg ctggcagcaa agacgaatgg caatggcaac
     1681 ggagctcgtg cgccgaagcc ggagatggcc gagccggtgg cgggggatta gctctcagtt
     1741 atccgaaact cagaaaaggc cgccgagagg cggccttaat tttttcattg gtctttgtta
     1801 gcccattaac ccctgtcgaa cacgcgaagg gtggagcagc cttgcctcct gactgtctcc
     1861 gcactcggtt acttcgagca catcttggcg aatgcaatgc gacaagtgtg gcgatttccg
     1921 gcccgaggtg ccggtgaaac caagcggttc gacgcgaacg agggacggcc agtgcaagaa
     1981 tgcgcatttc tgggtcgcga gcgaggcggt agcaggaatt gtacaaactc gtgacaatgt
     2041 cctgacaaac gggcacgacc gggcgccgta gcatcgcggg agtcggccgg atcagagaaa
     2101 aagtcgcgtt ttgctgcagg atcctatcag cgtcctcccc tccttggtcc ggcacaaagg
     2161 agattcacat gccacgacgc gtgctcgtcc tcactgctgg tctcggtttc gctttgctgt
     2221 tcctgtccag cgcggccatg gcgcagtaca agctcaggaa tctggattcg aatcaggtga
     2281 atggcgcagg ccacgacgac cccctcatcg tcaacgcgtg ggggatcgtt cacgggcctg
     2341 gcactccctg gtgggtcagc gacaacacct caggatggtc gacactgtac gacggaacgg
     2401 ggaaggcaca gcccctgcgg gtgctgattc ccaccgccgg caatgggccg gcatcgccga
     2461 gcggaaataa cggaccgggt tctcccacgg gcgtggtatt taacccgact gcatttacga
     2521 aggcggccga gtttcaggtg ggcagatggc cgtcgctgtt catctttgcc acactcgatg
     2581 ggacgatcag cggctggacg ttcaaaacca actccaatca ggcggtcatc ggctgggata
     2641 actcgagcaa aggatcttcc tataccggcc tggcgatcac caatcgggcc agcggcaacc
     2701 tgctatttgc cgccgacaat gtgaacggaa aggtggacgt gtacgacgcg accttcactc
     2761 ccgtgaacat gcctggcgca ttcagcgatt ccgttgctat tccggcgggc ttcgcgccgt
     2821 ttggaattcg ggacatcgac gggatcgtct atgtgatgtt tgccagcacc accggcggca
     2881 agggtggttt tgtggagcag ttcaaggaag acggcacgcc ggtcacgccg ggcaaagcgc
     2941 tgatctcggg tacgccgctg aatcagccgt ggggtatcgc agtggcgccg aagaacttcg
     3001 gagtgctgag caacacgctg ctgatttcga acaacaccaa ctttgggacg atcaacggat
     3061 tcaatccagt taccgggcag ttcgtcggca cgatgaggga ctttgcaacg gggaagccga
     3121 tcgtgatcga tcagttgtgg ggaatcgatt tcggcgacgg actgggcgga aatgggccag
     3181 ccagcgaact gtacttcgcc gccgggccga gcaacaatct ggctgggacg ttcgggtcga
     3241 tcgtagtcaa gtagagaacg cgcctatcga gcacaaaggc cgccactggc ggccttttcg
     3301 ctgatttccc aatactaaga gccgacttct tcagaagatc agggacgagc tgtcaataga
     3361 tcggtttcaa ctgcatcgca ggagcaacag atggagaggg ttcatccaga gagggtggca
     3421 atgttagcaa tcagctatac caaatatcat actgagcatt cagaaaagcg ttttcagacg
     3481 atagttaaca gcgcgcaacg aaatgtatag atgctgatag accaaagctt acggctgagt
     3541 gttatggaga gttgattgct ctcaccacaa gccgtcttgg ctccctgcat ggcctctgaa
     3601 tagctggcgc agaacttgag ccgcgccagc gtttacttgc tccaaaagtg tcgcagtttc
     3661 tcaggagata acgatgacat cagttcttcg ttctgtattg gtgcgtggtt gcggtgctgt
     3721 gctggcgctt gccctaatgt gtggactctc gacgttgctg agggcgcaag acgtcgcttc
     3781 gctgacgggc gtggttacag atgccaccgg ggcggtggtg cccgacgtcg atgtgaaact
     3841 gcttgatacc aagaccaacg ccactttcga aaccaagacc aactcgctgg gggcctatca
     3901 gtttgtaaag cttctccctg ggccagggta taagctcaca tttagtaagc agggtttcag
     3961 cacattaacc attcctgaca tctaccttgc cacgggaaac agccacactc aggacgccca
     4021 cttggcggta ggagcactct ccgagacggt ggaagtaaag ggggagggcg gcgcagtttc
     4081 ccttgacacg accgatacgg cggtgagcac tgatttcgac atgacgctga cccacgaact
     4141 tccagtgcag cttcgcgaca acccggctgc gctgatgact ttgcagccgg gggtggtctc
     4201 cgttggcgct ggcagtggca gcgacacgaa cttcacacga gccggagctg tcaccggtgc
     4261 tcgcgacgat cagaacaatg tgactgtcga tggccttgat gtcaacgact ttggaaccgg
     4321 ccaggccttc gtcgtgaatg gcaacgcccc ggtcgactcc atccaggaat ttcgcggcga
     4381 aagcgccaac cctttgtctg ccgaaggacg aggcagcggc gcacagattg ctctcaccac
     4441 caagagcggc accaacacgt ggcatggagc agccgacgag tacaatcgcg tgcgagccta
     4501 cgctgccaat cgatacttta acaacctcaa tggattgccg cggactccgc tcatccgcaa
     4561 ccagttcggt gccgaggtgg gcggaccggt cctcaaggat aagctgttct tcttcttcga
     4621 ttattttggg cggcgggata accagggagt ttccgtcgag aacatcgtgc cgctcgatac
     4681 gtggcgcggg gacggtacta gttcgactcc cggaatcccg caggtcggtt acatcaacaa
     4741 cggagcgggt tgcaccaacg ccagccgggc gaacactacc ccgggttgca tttcttacat
     4801 tccgaatact gcagccagcg ggcagacgct aaccggcttg gatcctcagg gaaccggtag
     4861 cagcagtgca ctgctcgcgt tcatcaatgg acgctatcca catgctaacg atctaggcgt
     4921 tggcgacggg atcaacacgg gcggattccg cttcaacacg ccggcgcatc tcaccgaaaa
     4981 cgcgtacatc gggcgtattg actacaatct gtcaagcaag atgagattat tcggtcgctt
     5041 cactatcctg cggcagaaca ccgacgacga cgttaacttc cccgcgcccg tacaatttcc
     5101 gggcgatcct ctaacgcacg cgattatcga taccagctat gcctatgtga tcggacacac
     5161 gtggacaatc tcgaatacaa aggtcaacca attcgtttac ggcgaaacca agcagcaact
     5221 gaacttcccc acgctgttta atcccaccgg gttcatccag aatcagaatt ttggcaacgt
     5281 caccgccccg ttctctaacc cggcgtcgca gggccgaacg gttccgat
//
LOCUS       DASPUL010000429         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_488877, whole genome shotgun sequence.
ACCESSION   DASPUL010000429 DASPUL010000000
VERSION     DASPUL010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488877"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VEJ00_10285"
     CDS             complement(<1..392)
                     /locus_tag="VEJ00_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXX01592.1"
                     /translation="MKPKRTILCVDDNEQSLSHRKIMLETRGYRVAAFSRGEEALARF
                     HQGDIDLVIADMAMPGLDGPQLIARIKEFAPQTPAILISSKVRIYDHDSQADVFLTKG
                     MYSPADLLERVRLLLVRKRGPKRLQHRTA"
     gene            complement(601..1497)
                     /locus_tag="VEJ00_10290"
     CDS             complement(601..1497)
                     /locus_tag="VEJ00_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HXX01593.1"
                     /translation="MPRQRLAVAQWDVFSSKVLEGNSLAVIFDGRGLSDATMQALAKE
                     MNLSETTFVFPRDAELERERGVRVRIFTVQEELPFAGHPTLGTAFALRAETQAKEIAL
                     ELNVGRIPVRFEDAPGEPVFGEMTQMNPVFGMQHDREALAQATGLRVEDFDPSLPVET
                     VSTGLAYAIVPLKSLRVVQELRVNLNSGYLEKTESKFFYFVTRETVDPQARLHARMLF
                     YGGEDPATGSAAGAAAAWMVARWVAQPDERVLIEQGLEMGRPSQIFVRASLLQGGRSD
                     NRVVNVRVGGHAIEVMRGEILL"
     gene            1842..2525
                     /locus_tag="VEJ00_10295"
     CDS             1842..2525
                     /locus_tag="VEJ00_10295"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXX01594.1"
                     /translation="MSPTATETYGIPRINDTAPDFTADTTQGPIRFHEWIGDGWAILF
                     SHPKDFTPVCTTELGYMAKLQPEFAKRNTKIIGLSVDPVASHQKWEGDIEETQGAKVN
                     YPMIGDPQLKVAKLYGMLPGDAGESCEGRTPADNATVRTVFVIGPDKKIKLQLSYPMT
                     TGRNFDEILRVIDSLQLTARHKVATPVNWKPGEDVIITAAVSNEEAAQKFPGYKTVKP
                     YLRVAKQPK"
BASE COUNT          613 a          898 c          860 g          582 t
ORIGIN      
        1 gccgtccggt gctgcaggcg cttgggcccg cgcttgcgca ccagcagcaa gcgaacgcgc
       61 tccagcaaat ctgccggaga gtacatcccc ttggtgagga agacgtcggc ttgcgagtcg
      121 tggtcgtaaa tgcggacttt gctcgagatg aggatagccg gcgtctgggg cgcgaactcc
      181 ttgatcctgg cgatcagctg cggaccgtcg agccctggca tcgccatgtc ggcgatcacc
      241 agatcaatat cgccctggtg gaagcgggcc agcgcttcct ctccgcgcga gaacgcggcg
      301 acccggtagc cccgggtttc gagcataatc ttgcggtggg agagcgactg ttcgttgtcg
      361 tcgacgcaga gaatggtgcg cttcggtttc atctttgttc tgcaacccac tggcgcgatg
      421 gaagctgccc atcgcaaacc ggtagaagtt gttttcaatg tgaaggtgtt aagcgagatg
      481 catgctaacg tttgttacaa acgcctgtaa agtgccgccg gtgccaatgg aactttctgg
      541 ccattgattt tattttcggc gggtgcaccg tcgcattttt cgcggttcgc atataccact
      601 ttagagcagg atttctccgc gcatgacttc aatggcgtgg cctcccacgc gaacgttaac
      661 cacgcggtta tcacttcgcc cgccctggag aagagaagcc cggacaaaaa tctggctggg
      721 gcgccccatc tccagacctt gctcgatgag cacgcgctcg tccggttgcg ccacccagcg
      781 cgccaccatc cacgcagcgg cagctcccgc agccgagccg gtggctggat cttcgccgcc
      841 gtagaacagc atgcgcgcat gcaagcgtgc ctgcggatcg accgtctccc gcgtgacgaa
      901 gtagaagaac ttgctctcgg ttttctccag gtaaccggag ttcaggttca cacgcaattc
      961 ctgcacgacc cgcagcgact tcagcgggac aatcgcgtac gcgagtcccg tcgacacggt
     1021 ctcgacggga agagacggat cgaagtcttc cacgcgcaat ccggtcgcct gcgccagggc
     1081 ctcgcgatcg tgctgcattc cgaataccgg attcatctgc gtcatctcgc cgaatacggg
     1141 ctcgcccggc gcatcttcaa agcgcacggg gattctcccc acgttcagct ccagcgcaat
     1201 ctcctttgcc tgcgtctcgg cgcgcagcgc gaaagccgtt cccagcgtgg gatgtccggc
     1261 gaatggcagt tcctcctgca ccgtgaagat tctcacccgt acgccgcgct cgcgttccag
     1321 ttccgcatcg cgcggaaaga cgaaagttgt ctctgacagg ttcatctcct tcgccagcgc
     1381 ctgcatggtg gcatcgctta gcccacgccc atcgaagatc acagccagcg aatttccctc
     1441 gaggactttc gacgaaaata catcccactg cgccacggcc agtcgttgtc tgggcattcg
     1501 tttctccggg aaggcgcgaa tccagcgcca acagcagtga tgtcggccgg gctggcaacg
     1561 atgcaggaaa gacgcccgcc atcgcctccg gcgcgccgct ggctgtttga cactggcgct
     1621 ttgcgcttct atcctaaatc ctatgttcat tccctccacg accgccgcca agtgttgttg
     1681 tcgacggccg ccggcgtgtg ggtgaggaac cagcaattct tccggcccac cccgagcgag
     1741 cgggtgggct tttactttcc atccgtttcg cagcaagcag aatagctggg gtagcaactt
     1801 tcggaagctt gcaccaaatc tgacttcatg aaggagaaac catgagcccg actgcaacgg
     1861 aaacgtacgg tattcctcgc atcaacgaca ctgctcccga tttcaccgct gacaccaccc
     1921 agggcccgat ccgttttcat gagtggatcg gcgacggctg ggccatcctg ttttcccacc
     1981 ccaaggattt cacccccgtc tgcacgaccg aactgggcta tatggccaag ctgcagccgg
     2041 aattcgccaa gcgcaacacc aagatcatcg gcctgagcgt cgaccccgtc gcaagtcacc
     2101 agaagtggga aggcgacatt gaagagactc agggcgccaa ggtgaattat ccgatgatcg
     2161 gcgacccgca gctgaaagtt gccaagctct acggcatgtt gcccggcgac gccggcgaga
     2221 gctgcgaggg ccgcactccc gctgacaatg caaccgtgcg cactgtcttc gtgatcggtc
     2281 cggacaaaaa aatcaagctg cagctcagct atcccatgac cacgggccgc aacttcgacg
     2341 agatccttcg cgtgatcgac tcattgcagc tcaccgccag gcacaaggtt gccacgccgg
     2401 taaactggaa gccgggcgag gacgtcatca tcaccgccgc ggtttcaaac gaagaagccg
     2461 cgcaaaagtt cccgggatac aagacggtga agccgtatct gcgggtggcg aagcagccga
     2521 agtgagcggc atctggacgg acgaatgtgg cgcgggcgtc gaagcccgcg ccgtttttgt
     2581 ctacactagg aagggttgag cgcggaagcg catgcggtcc ggtgatcgtc ccggtcttca
     2641 aagcggccta gacgaagcgg gctgtaatgt tttcagcgga ttgctgatca tcaaaagcgg
     2701 ctaagttgct tcaaaacggg cgtgggcgga aggaaaatgc aaccaatatg caacggcggg
     2761 attcgctcac gcttcgcttg gaacggtcga agtgaccggg acgaaaggga agagaatttt
     2821 cagagggctc tgccatgcct gaggaaaagg aaaaggcgtc gccgctgcgt ccaccaccga
     2881 ggtccgtcag gaaagatcct cactttgacc tattgatttg aagtcgcgat tgtggagtga
     2941 agaatggttt gcc
//
LOCUS       DASPUL010000430         3841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1447023, whole genome shotgun sequence.
ACCESSION   DASPUL010000430 DASPUL010000000
VERSION     DASPUL010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447023"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VEJ00_10300"
     CDS             <1..411
                     /locus_tag="VEJ00_10300"
                     /inference="COORDINATES: protein motif:HMM:NF012947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01595.1"
                     /translation="VEHPEAVLKAERMVAAEAQAFRGALQAESVVPTIVALRHRLDEI
                     CRQELESFTKERGPFTREQDRTFHALVTQVIQKIASSLALELKEPPEKEEQERMAAVV
                     ARLFHLEAPKPAPAGTRSENPKHEQGPEPAVTVH"
     gene            374..1054
                     /locus_tag="VEJ00_10305"
     CDS             374..1054
                     /locus_tag="VEJ00_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX01596.1"
                     /translation="MSKAQNPQSQSTDPNSRAGKPAGSNAPAGNWTNAQAYMLAAFCL
                     LLGVVLGYLFRGSASPAAARANLQQSATPAQPAPDPNAQAALAQATAPLLDAVNKDPN
                     DYDSLVKLGDIFYDGQQYASAIQYYERALGLHPENPDVRTDMGTAYWYTGDADKAIAA
                     METSLKYRPGHPQTLFNLGWVRWQGKGDPKGAVEAWEKLLKTNPDYPQRQQVEQYIAK
                     AKEHAARG"
     gene            1258..2481
                     /locus_tag="VEJ00_10310"
     CDS             1258..2481
                     /locus_tag="VEJ00_10310"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXX01597.1"
                     /translation="MHPQLLPILPAPPARRRSDRVSISFPVEIAAIDAKGEAFTEATK
                     TTTVSRYGCCVALSRRLDPNQKLQLRRTDTNASAEAHLVGAMSAPGEPLLYGLSVNDV
                     CDNLWGIRFSSCDERLLDSLSDGVYFVNREREITFWSEGAEKVAGFTASEAVGKYCYN
                     NLLRHVDDHGRPLCTDGCPLSRVMLDGEPHEVNVYLHHKEGHRVPVSVRAQPVRNSAG
                     QIVGAIEVFTSTASDFPAPEMPDKAPLSPNHDPATGLPNRRYLKLKISQAIEERQHLG
                     KLWGLLLLSVDGFDRISDTHGPQTRDRLLATMSLTLIQCLKLPKLVGRWADDQFLALV
                     PDGNAAALAEAAERCRALIACSGVRAHSSHVSATVSVGTTMLSPSDTPRSAMERAEDL
                     LNTSMRAGGDCITAG"
     gene            complement(2497..>3841)
                     /locus_tag="VEJ00_10315"
     CDS             complement(2497..>3841)
                     /locus_tag="VEJ00_10315"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXX01598.1"
                     /translation="QHESPDLILLDVRLPGLNGLQVLEQLKKNGDTASVIMITADPQL
                     DDVKSALKLGAYDFIGKPLDFEELRVTIQNALETTRLRSEVQSLRGEVRRRTGYHDVV
                     AVSPKMTELMSFVQKVAASEATTILIQGESGTGKDLVAKTIHYESTRQDGPFVAINCS
                     AIPETLMEAELFGHEKGAFTDAKQMKKGLFETANGGTLFLDEIGELSPLLQAKLLRVL
                     EDQVIRRVGGVRDLQVDVRVVAASNRDLEKAVREGQFRQDLYYRLAIIAIFIPPLRER
                     SEDILPLVDFFISLYNRKFKKAVRGISPETRRLLLAHNWPGNVRELKNSIERAMILED
                     EAVLRPMYMPFAVGEAARSGLTAFEHTSAATDGVGKQLASGRVLPRLYIPEEGTSLEE
                     VERAMVELAMRQANGNQTHAARLLDISRDALRYKLKKFDLVPGDEETADSIPSES"
BASE COUNT          737 a         1247 c         1152 g          705 t
ORIGIN      
        1 gtggagcatc ccgaggcagt cttgaaggcg gagcgaatgg ttgcggccga ggcgcaagcc
       61 tttcgtggcg cactgcaggc cgagagtgtc gtgcctacga tcgtggcgtt gcgccatcgc
      121 ctggacgaaa tttgccggca agaactggag tccttcacca aagagcgcgg gccttttacc
      181 cgggagcagg atcggacgtt tcacgccctg gtaacgcaag tcatacagaa aattgccagc
      241 tccctggccc tcgaactcaa ggaacctccg gagaaggagg agcaggaacg catggcggcc
      301 gtggtcgccc gcctgttcca cctcgaggcg cccaaaccgg cgccggccgg caccagatcg
      361 gagaacccaa aacatgagca aggcccagaa cccgcagtca cagtccactg atcccaactc
      421 tcgcgccgga aaaccagccg gaagcaacgc gccggccgga aactggacga acgcgcaggc
      481 ctatatgctg gctgctttct gcctgttgct gggcgtggtc ctcggctatc tgtttcgcgg
      541 atcggcatcg ccggccgccg cgcgggcgaa cctccagcag tcggcaacgc ctgcgcagcc
      601 ggcacctgac ccgaacgcac aggctgccct ggcccaggct acggctccgt tgctcgacgc
      661 cgtgaacaag gatcccaacg actatgactc gctggtgaaa ctcggcgaca ttttctatga
      721 cggccagcaa tacgcgagcg ctatccagta ctacgagcgc gcgctcgggc ttcatcccga
      781 aaatcccgac gtccgcaccg acatgggcac cgcgtactgg tacaccggcg atgccgacaa
      841 ggcgatcgct gccatggaaa cgtcgttgaa gtaccgccct ggacatccgc agacgctatt
      901 taatctgggc tgggtgcgct ggcagggcaa gggtgatccc aagggagctg tcgaggcgtg
      961 ggagaagctg ctgaaaacca atcccgacta cccgcagcgg cagcaagtcg agcagtacat
     1021 tgccaaagcg aaagagcacg ccgcccgcgg ataacgtatc cgatgagcag caccggtttg
     1081 ccggaaccgc aataacttcg aaaatagggc gcttttcata cctcctcaga acgcgccgcg
     1141 gggcttccgc aaggaagccc cattttccgc tccgaggccg ctgctattag gctcaattaa
     1201 tgaaggatgt tgagcctttc actaatccgt cccggctccg ggccgatgtg ttcggttatg
     1261 catccacagc tacttccgat cttgcccgcg cctccagcac gccgaaggag cgaccgcgtt
     1321 tcaatcagct ttccagttga gatcgccgcc atcgacgcca aaggagaagc cttcaccgag
     1381 gcgaccaaaa ccaccaccgt cagccgctac ggttgttgcg tcgcactctc gcggcggtta
     1441 gatcccaacc agaagctgca gctgcggcgc accgatacca acgccagcgc tgaggcgcat
     1501 ctggtcggcg cgatgtctgc cccaggagag cccctgctat acggcttgtc cgtcaacgac
     1561 gtttgcgaca acctgtgggg aatccgcttc tcctcttgcg acgaacggct gctcgactcg
     1621 ctgagcgacg gtgtctattt cgtaaaccgg gagcgcgaga tcacattctg gagcgaggga
     1681 gcggagaagg tcgcgggttt taccgccagc gaagctgtcg gcaaatattg ctacaacaac
     1741 ctgctaagac acgtggacga ccatggcagg ccgctctgca ccgacgggtg tccgctgagc
     1801 agggtaatgc tcgatggcga gccgcacgag gtaaacgttt atctccatca caaggaaggg
     1861 caccgcgtac cggtttccgt gcgggcgcag cctgtacgaa acagcgccgg ccagatcgtc
     1921 ggcgccatcg aagtcttcac cagcacagcg tctgactttc ccgcgccgga aatgcccgac
     1981 aaggcgccgt tgagcccgaa tcatgatccg gccaccggtt tgcccaatcg ccgctacctg
     2041 aagctgaaaa tctcgcaggc gatcgaggag cgccagcacc tgggaaagct gtggggcctg
     2101 ctgctgttga gcgtggacgg gttcgatcgc atcagcgata cgcatggtcc gcaaacgcga
     2161 gaccgcctgc ttgcgaccat gtcccttacc ctgatccagt gtctcaagct cccgaaactg
     2221 gtggggcgct gggcggacga tcagtttctt gccctggttc ccgacggaaa cgccgccgca
     2281 ttggccgaag ccgccgagcg ctgccgcgcg ctcatcgcct gttcgggagt gcgggcgcac
     2341 agttctcatg tgtcggcgac ggtctcggtc ggcacaacca tgctctcgcc ttccgatacg
     2401 ccccggtccg ccatggaacg cgctgaggat ctgttgaaca cgagcatgcg cgcgggaggt
     2461 gactgcatca cggcgggata ggtcaccgcg ccggagctag ctctccgaag gaatcgagtc
     2521 ggcagtttcc tcgtcgcccg gcaccaggtc aaacttcttc agcttgtagc gcagggcgtc
     2581 ccggctgatg tcaagcaacc gcgcggcgtg cgtctggttg ccgttggcct ggcgcatcgc
     2641 cagctcgacc atcgcccgct ctacctcttc cagagacgtg ccttcttccg gaatgtagag
     2701 ccgcggcaga acgcgtccac tggccagctg tttgcccacg ccatccgtcg cagcgcttgt
     2761 gtgctcgaac gcggtcaggc cgctgcgcgc ggcctctcca accgcgaagg gcatgtacat
     2821 ggggcgaagg acggcttcat cttcgagaat catggcgcgc tcgatcgagt tcttcagttc
     2881 gcgcacgttt cccggccagt tgtgcgccag tagcaggcgc cgggtctccg gcgaaatgcc
     2941 gcgcaccgct ttcttgaatt tccggttgta cagggagatg aagaaatcga ccagcggcaa
     3001 gatgtcctcg ctgcgctcgc gcagcggcgg aatgaagatc gcgatgatcg ccagccggta
     3061 gtacagatcc tgccgaaact gcccctcgcg caccgctttt tccagatcgc ggttcgatgc
     3121 cgccacgacg cgcacatcga cctgcagatc gcgcacgccg ccgacgcgcc ggatcacctg
     3181 atcttccagc actcgcagca acttggcttg cagcagcggc gacagctcgc cgatctcgtc
     3241 caggaaaagc gtgcccccgt tcgccgtctc gaacaggccc ttcttcatct gcttggcgtc
     3301 ggtgaaggcg cccttttcgt gcccgaacag ctcggcctcc atcagcgttt ccgggatggc
     3361 cgagcagttg atggccacga acggaccgtc ctgccgcgta ctctcgtagt gaatggtctt
     3421 ggcgaccaga tccttgcccg tgccgctctc gccctggatc agaatcgtcg tggcctcgct
     3481 ggcggccact ttctgcacga agctcatcag ctctgtcatc ttgggcgaga ccgcgaccac
     3541 gtcgtgatag cccgtccgcc gccgtacttc gccgcgtaag gactgcacct cgctgcgcag
     3601 ccgcgtggtc tccagcgcgt tctggatcgt aacccgcaat tcctcgaagt ccagcggctt
     3661 gccgataaag tcgtacgcgc ccagcttgag cgcactcttg acgtcgtcca gttgcgggtc
     3721 ggcggtgatc atgatgaccg atgcggtatc gccgtttttc ttgagctgtt cgagaacctg
     3781 caggccgttc aaaccgggca ggcggacgtc gagaagaatc aggtcgggag actcgtgctg
     3841 g
//
LOCUS       DASPUL010000431         3123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1725260, whole genome shotgun sequence.
ACCESSION   DASPUL010000431 DASPUL010000000
VERSION     DASPUL010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725260"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="VEJ00_10320"
     CDS             <1..944
                     /locus_tag="VEJ00_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXX01599.1"
                     /translation="YPGFGPKRIIGRDGKVAALETLKTKWVFDENRRFNPAFFEGSES
                     QIECDTIIMAIGQAPRLDFFTQEDEVELSPRGLIAVNPQTLMTSAPGIFAGGDCVFGP
                     RLIIDSVGDGKRAAVGIDEYLRGGKHPEPIIEVEVLKRHAMTLGYLDISRQVVPMLPL
                     ERRTGVTEVEVGYNANEAIAEAQRCLHCWVNTIFEGSPEDGSMCILCGGCVDVCPEKC
                     LELVSLDRIGFEPEAVRHIRQNQECFGVELNEVKADELGVVTGAAMLKDETRCIRCGL
                     CAMRCPVGTITMESYNLLPAEPTGLISVAAIGAGLQK"
     gene            959..1459
                     /locus_tag="VEJ00_10325"
     CDS             959..1459
                     /locus_tag="VEJ00_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HXX01600.1"
                     /translation="MAEGKNNSSADLAKDLNRREFFTKLGLGSVSIAAVGTAAFSYQY
                     LEPNVLYEPSPVVNAGKPDRYPLDSVTLDVNNGIYIIHSQEGYFSLSAVCTHLGCLTA
                     WKPELGIIACPCHGSKFEQSGKKIEGPAPKPLPWKRIWLNDDGDLLVDRSTDVLPLAR
                     DSFVRV"
     gene            1475..2251
                     /locus_tag="VEJ00_10330"
     CDS             1475..2251
                     /locus_tag="VEJ00_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HXX01601.1"
                     /translation="MAKTVEQYFEELQGTRVWRSIFRSGTGSSTLKRALAIQQNVFLH
                     LFSTKVRTRMLTFSATWYLGTLTLGTFLLLVATGIPLMLYYHPSVPQAYADTKDLQFV
                     VSSGLFLRNLHRWSAHAMVFLVFAHMFKVFYRGAYRTPREFNWVIGVVLLLITLLLSY
                     TGYLLPWDQLAFWAITVGSNIASAVPVIGNKIHFLLLGGNAVNANALLRFYVLHCMIL
                     PLAAIFFVAIHFWRIRKDGGLYAHSSEPAVMRVQAAKEGQ"
     gene            2255..2959
                     /locus_tag="VEJ00_10335"
     CDS             2255..2959
                     /locus_tag="VEJ00_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01602.1"
                     /translation="MADKDFIAGVDSNAKKSPRRIVFITRRTSAQVKAETEDQIQTFP
                     EVLFRAAVAIMTLAVALVWIALLFNAPLEGLADPSHTPNPAKAPWYFLGLQEMLHYFP
                     PVVAGVLAPGLVVMALIVIPYFKVNIEAEGLFLKDRQKRLRIFYVVAAALSVFLIAFR
                     VYAALVPTLIMAAVMLLAAQSSPQSLSAFRRYLAARPLSYWVMTWFLFELVVLTAIGT
                     FFRGPGWSWVWPWQGS"
BASE COUNT          587 a          892 c          982 g          662 t
ORIGIN      
        1 tttatcccgg gtttggcccg aagcggatca ttggccgcga tggaaaggtc gccgcgctcg
       61 aaacgctgaa aaccaagtgg gtcttcgacg aaaaccggcg gttcaacccg gcgttttttg
      121 agggcagcga gagccagatc gagtgcgaca cgatcatcat ggcgatcggg caggccccgc
      181 gcctcgattt cttcacgcag gaagatgagg tcgaactctc tccgcgcgga ttgatcgccg
      241 tcaatccgca gacgctgatg acctccgctc ccggcatctt tgccggtggc gattgcgtgt
      301 tcgggccccg cctgatcatt gacagcgtgg gagacggcaa acgggcggcc gtcggcatcg
      361 acgaatacct gcgcggaggg aagcatcctg aaccgatcat cgaagtggaa gtgctgaagc
      421 ggcacgcgat gacgctgggg tatctggaca tcagccgcca ggtggtgccc atgctgccgc
      481 tcgaacgccg gaccggcgtg acagaagtcg aagtcggcta caacgcgaac gaagcgatag
      541 ccgaggcaca gcgctgcctg cattgctggg tcaacacgat cttcgagggc tcgcccgagg
      601 acggcagcat gtgcatcctg tgcggcggat gcgtggatgt gtgtcccgaa aaatgtctgg
      661 aactggtgtc gctcgaccgc atcgggttcg agccggaagc ggtgcggcac attcgacaga
      721 accaggaatg ctttggcgtc gagctgaatg aagtgaaagc cgacgagctg ggagtggtca
      781 ccggcgcggc catgctgaag gacgaaaccc gctgcatccg ctgcggcctg tgcgccatgc
      841 gctgtccggt gggcacgatc acgatggagt catacaacct gcttccggcg gagccgaccg
      901 gactaatttc ggttgccgcg atcggcgcgg gattgcagaa gtagacgaga gagaaatcat
      961 ggcagaggga aaaaacaact cgagcgcgga tctggccaag gacctgaacc ggcgggagtt
     1021 tttcaccaag ctcgggctgg gctcggtcag cattgcagcg gtcggtacgg cagcgttttc
     1081 ttatcagtat ctcgagccga acgtgctcta cgagccgtcg ccggtcgtga acgcggggaa
     1141 gccggaccgt tatccgctcg actccgtcac gctcgacgtg aacaacggca tttacatcat
     1201 tcactcgcag gaaggttact tctcactcag cgcggtctgc actcatttgg gctgtctgac
     1261 agcgtggaag ccggagttgg ggatcattgc ctgcccctgc catggcagca agtttgagca
     1321 gagcggaaag aagatcgagg ggcctgcgcc caagccgctg ccctggaagc gcatttggct
     1381 gaacgacgac ggcgatcttc tggtcgatcg ctcaaccgac gtgctgccgc tggcgcggga
     1441 ttccttcgtg agggtctagc ttgaggaaat cagcatggcg aagactgtag agcaatattt
     1501 cgaagagttg caaggcacgc gggtctggcg ctcgattttc cgcagcggaa ccggatcgag
     1561 cacgctgaag cgcgccctgg ccattcagca gaacgtgttt ctccacctgt tctcgaccaa
     1621 ggtgcgcaca cgcatgctca ccttcagcgc gacatggtat ctgggcacgc tgacgctggg
     1681 gacgtttctt cttctggtcg cgaccggtat tccactgatg ctgtactacc acccgtccgt
     1741 gccgcaggcc tacgccgaca cgaaggactt gcagttcgtt gtctcgtcgg gattgttcct
     1801 gcgcaacctg catcgctggt ctgcgcatgc catggtgttt ctggtgtttg cccacatgtt
     1861 caaagtgttt taccggggcg catatcgcac gccgcgggaa ttcaactggg tgatcggcgt
     1921 cgtgcttctg ctgatcacgc tgcttttgag ttacaccggc tacctgctgc cctgggatca
     1981 gctggccttt tgggcgatca cggtggggtc gaatatcgcg tcggcggtcc cggtgatcgg
     2041 caacaagatt cacttcctgc tgcttggagg caacgcggtc aacgccaacg cgctgctgcg
     2101 tttctatgtg ctgcactgca tgattctgcc gctggccgcg attttcttcg tggcgattca
     2161 cttctggcga attcgcaagg acggcgggct gtatgcccat tctagcgaac cggcggtgat
     2221 gcgggtgcag gcggcaaagg aggggcaata ggtcatggcc gacaaggact tcatcgccgg
     2281 agtcgactcc aacgcaaaaa agtcaccgcg ccggattgtt ttcatcaccc gccgcacgtc
     2341 ggcgcaggtg aaggccgaga ctgaagatca gattcagacg tttccggaag tattgtttcg
     2401 cgccgcagtc gcgatcatga cgctggcggt ggcattggtc tggattgcat tgctgttcaa
     2461 cgcgccgctt gagggcctgg ccgatccatc gcacacgccg aatccggcga aggcgccctg
     2521 gtacttcctg gggctgcagg agatgctgca ctactttccg ccagtggtgg cgggcgtgct
     2581 ggctccgggc ctcgtggtga tggcactcat cgtcattccc tatttcaagg tcaacatcga
     2641 agcggagggg ctttttctca aggaccgcca gaagcggctg cgcattttct acgtggtcgc
     2701 cgcggcgctg tcggtctttc tgatcgcgtt cagagtgtac gcggcgcttg tgccgaccct
     2761 gatcatggcg gcggtcatgc tgctggcggc ccagagttcc ccgcaatcac tctccgcttt
     2821 ccggcgatat ctcgcagcca gaccgctttc atactgggtg atgacttggt tcttattcga
     2881 actggtggtg ctcaccgcca tcggtacatt tttccgtggg ccgggctgga gctgggtctg
     2941 gccgtggcaa ggctcataac atgctcataa ggatacggcg atcatgaacg acaaatcccg
     3001 gtcgctctgg caaaaactct tcggagacag cgtgcgcgcg tttggcgttg tcagcctggt
     3061 ttttctgctt tcactcgcga tcgcaccggc gaaaaacttc ttcagcgagt ggcgccacta
     3121 cca
//
LOCUS       DASPUL010000432         4297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_78366, whole genome shotgun sequence.
ACCESSION   DASPUL010000432 DASPUL010000000
VERSION     DASPUL010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78366"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1557
                     /locus_tag="VEJ00_10340"
     CDS             <1..1557
                     /locus_tag="VEJ00_10340"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXX01603.1"
                     /translation="PAAVNIDWYRSRNKTLSGAGLDPAVVPGAIHAALTVLERWGTMS
                     FEDVATPAIGYAENGFPMRQSTANAIRNQLKLFEQWPDNQKFWLKPDHSLYTPGETIA
                     LPTLARTLRRMVEAERAARSKGRAAGIEAARNLFYKGDLAREMVAFLKKSGAPFELSD
                     FSEFFARVEGPAKTTYRGYTVYKHGFGSQGPVLLQTLNILETFDLHGMGYGSADYLHT
                     ITEAMKLAYADRDTYYADPAFVDVPGEGLLSKTYAKERAKLIDPKRASKSFIAGDPLP
                     YDSKVKQWPYWKADLKDASNDRAGAEPSPLERTASLGLDSTGVSKDTTHMTVIDKEGN
                     IFDATPSGGWIPGAVILGDTGIGMSVRGEQFWLDSTRAAQIRPHARPRYTLTPSIVFK
                     GDVPIMGIGTPGGDNQDQTILQAFLSVVEFWDNWYPNLHAAFERPRVQTLHFYGSFWP
                     HAAGFNKLNVEATIPDSVYDELKARGHDVSRLRAFGMSGCATGVMIDPATQNRFAAGD
                     PRRDCYAIAY"
     gene            complement(1579..1842)
                     /locus_tag="VEJ00_10345"
     CDS             complement(1579..1842)
                     /locus_tag="VEJ00_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01604.1"
                     /translation="MKKLLVVALSCVWFGNLAHAADSSCDAQAAEKKLSGAAKNSFVK
                     KCQKDAAAAAKTACESQAAEKKLAGAAKNSFVKKCEKDAAAPK"
     gene            complement(2096..2302)
                     /locus_tag="VEJ00_10350"
     CDS             complement(2096..2302)
                     /locus_tag="VEJ00_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01605.1"
                     /translation="MVPNSQEPRVYVVALSSEAAPEIYAFLTEELDVAIPSGTDPLVL
                     TANEAAAVVRLARELAPLDAEVGR"
     gene            complement(2442..3017)
                     /locus_tag="VEJ00_10355"
     CDS             complement(2442..3017)
                     /locus_tag="VEJ00_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01606.1"
                     /translation="MRRGRERERERTGFCHDSAADSVSASGIRLALSYLWGDKSFNDT
                     QNADRTAVVSTHHIKGQVNTVSDISSAHYPSLFEALRLLPGIAPRQERDRLGRAKYQA
                     TRSPGHTVDFSGVQLTLTQLQELFIYDQQRRPRVSRAGAALLLFLFEEGAFPSIDGRK
                     VDGNPEVLGQYSKGLASVVALNSRRGSHRIG"
     gene            3184..3630
                     /locus_tag="VEJ00_10360"
     CDS             3184..3630
                     /locus_tag="VEJ00_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXX01607.1"
                     /translation="MNVQFLLRLLVPLLLLVGAGRALADAAGDVRQLEQERFAAYVQG
                     DVAVLDRIFADDLVYFHSNGLSDPKSAVLQSFASGDLKISRFEAEDLQVRAIGNVMVA
                     TGLVHVDLVNKGNAAKFDIRYTAVYANQGGQWRLVHIQNGRLPAKT"
     gene            3803..4087
                     /locus_tag="VEJ00_10365"
     CDS             3803..4087
                     /locus_tag="VEJ00_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01608.1"
                     /translation="MNRPLPVNEAEKQQECCWLRGEMARQRALGGYGTAMQDLATLES
                     RSSQAGCAAVRADVQPGAPAAGGGNFDTCYMNCKQFTNKSNDVCFDQCSK"
BASE COUNT          870 a         1295 c         1314 g          818 t
ORIGIN      
        1 cccgcggcgg tcaacatcga ctggtatcga tcgcgcaaca agactttgag cggtgcaggc
       61 ctggatcccg ccgtcgtgcc cggcgccata cacgccgcgc tcaccgtgct cgagcgctgg
      121 ggcaccatga gctttgaaga cgtggcgacc cccgcgatcg gctatgcgga aaacggattc
      181 ccgatgcgcc aaagcacagc aaacgcgatc cgaaatcaac tcaagctttt cgagcagtgg
      241 ccggacaatc agaaattctg gctcaagccc gatcattccc tgtacacgcc cggcgaaacc
      301 attgcgctgc caaccttggc gaggacactg cggcgaatgg tcgaagccga gcgcgccgcg
      361 agatcgaaag ggcgagccgc cggaatcgag gcggcccgga acctgtttta taaaggcgat
      421 ctagcgcgcg agatggtcgc gtttctgaag aagagcggcg cgccgttcga gctgagtgat
      481 ttctcggaat tcttcgcgcg cgtcgaaggg ccggcgaaga caacgtatcg tggctacacc
      541 gtctacaaac atggcttcgg cagtcagggg ccggttctgc tgcagaccct gaacatcctc
      601 gaaaccttcg acctgcacgg catgggctac ggcagcgcgg actacctcca caccatcacc
      661 gaagcgatga agctcgccta cgcggatcgg gacacgtatt acgccgaccc cgcgttcgtg
      721 gacgttcccg gggaaggcct gctctcgaaa acatacgcga aggaacgtgc gaagctgatc
      781 gatccgaagc gcgcgtcgaa atcgttcatc gcgggcgatc cgcttccata cgactccaaa
      841 gtgaagcaat ggccgtactg gaaggcggac ctgaaggatg cgtcgaacga tcgcgcgggt
      901 gcggaaccct cgcctctgga gcgcacggcg tcgctcgggc tcgattcgac tggcgtttcg
      961 aaagacacga cgcacatgac cgtcatcgac aaggagggca acattttcga cgccacgcca
     1021 agcggcggat ggatccccgg cgccgtgatc ctcggcgata ccggaatcgg catgagcgtg
     1081 cgcggtgaac aattctggct cgactccacg cgcgcagcgc agatccgtcc gcacgcacgt
     1141 ccgcgataca cgctgacgcc cagcatcgtg ttcaaaggtg atgtgccaat catgggcatt
     1201 ggcacgccag ggggcgacaa tcaggatcag acgatcctgc aggcgttctt gagcgttgtc
     1261 gaattctggg acaactggta tccgaacctg cacgcggcat tcgagcgtcc gcgcgtccag
     1321 acactgcact tctacgggtc gttctggccg cacgccgccg gcttcaacaa gctcaacgtc
     1381 gaggccacca ttccggattc cgtctacgac gagctcaaag cgcgcggcca cgacgtcagc
     1441 cggttgcgcg ccttcggcat gtccggctgc gcaacagggg tgatgatcga cccggcgacg
     1501 cagaatcgct tcgcggctgg agatccgcgc cgcgattgct acgccatcgc ttattgagct
     1561 cctacgtagt gcagcttctt acttaggcgc agccgcgtct ttctcgcatt tcttgacgaa
     1621 gctgttcttc gcagcgccag ccagcttctt ctcggcggct tgactctcgc aggctgtctt
     1681 agcagccgca gcggcatctt tctggcattt cttcacgaag ctgttcttgg ccgcaccaga
     1741 gagcttcttc tctgctgctt gcgcatcgca gctggagtca gccgcatggg cgaggttccc
     1801 gaaccagaca cagctcaatg caacgacaag cagcttcttc attgcgcctc ccttggagaa
     1861 ctcgttgaaa atcgcaccga gcgattacac gagtctgaac ttacggcaac cttacttgtg
     1921 cgtaaccaac ggtgcgcctt tccgccggct taccgataac ctggagtaag ggaagcacga
     1981 aaaatgtggt ccacaggcga atgggtggct tgggttctgg tggtcgtcgc catggcggcg
     2041 gccgagtggt cctgcttgta cacgttgttt gcctagcgcc tggcgtggct taggcttacc
     2101 gaccgacttc tgcgtcgaga ggcgcgagct cgcgagccag acgcaccact gcagccgctt
     2161 cattcgcggt gagcacgagc gggtcagtcc cggacggtat cgcgacgtca agttcctcgg
     2221 tcaggaacgc gtaaatctcc ggagcagctt cagaggaaag ggccacgaca tagacgcgtg
     2281 gctcttgcga atttggaacc accactcgga tcaacccagc ggggcccccg caaacaatca
     2341 gagcgatcct gtggaaggcc gagcgtattg acgctgtcgt gaccatgacg ttgtagcgcg
     2401 gcagtaggac acgctggttc agtcggattt tcagctggtg atcagcctat ccgatgtgac
     2461 cctcgccggc tgttgagtgc gaccacgctc gccaatcctt tcgagtattg cccaagaacc
     2521 tccgggttcc cgtcgacttt gcgtccgtcg attgaaggga acgcgccctc ctcgaacaga
     2581 aagaggagca atgcggcacc cgctcgagac acccgcggtc ggcgctgctg atcgtagatg
     2641 aacaactcct gcagctgggt cagtgtcaac tgcaccccgc taaaatcgac cgtatgaccg
     2701 ggcgagcgcg tggcttgata cttggccctt cccaaccgat cgcgttcctg gcgcggggcg
     2761 atgccgggca gcaggcgcaa cgcttcgaac aaggatggat agtgcgcaga ggaaatgtcg
     2821 gacaccgtgt tcacctgtcc tttgatgtgg tgtgttgaga cgaccgctgt ccgatctgca
     2881 ttttgcgtat cgttaaagct cttgtcgccc cataaatacg acaacgcaag gcgtatgcca
     2941 ctcgcggaaa ctgaatctgc ggcgctgtcg tggcagaagc ctgtacgttc gcgttcgcgc
     3001 tcgcggcccc gcctcatctg cgccgctgtg tagcggcgaa gcgatccagc agggtgggca
     3061 ctagcgatcc gacggaagat gaatcaccat cgccgagacg caagatgcga taggatgaag
     3121 gcaggtcgcg gctgtgcatc gtgaccgcgc atggcgggtc tcaaaatcga ataggaggag
     3181 ccaatgaacg ttcaattttt gttgcggttg cttgttccac tgctgttact ggttggcgcc
     3241 gggcgcgcac tggctgatgc tgcgggcgac gtccgccagc tcgagcagga gcgctttgca
     3301 gcttacgtcc agggtgatgt cgctgttctc gaccggatct tcgcagacga cctggtctac
     3361 ttccattcaa acgggttgtc tgatcccaag tctgctgtgc tgcagagctt cgcatcgggc
     3421 gacctcaaga tatcgcgctt cgaagcggag gacctccagg tgcgggcgat cggcaacgtc
     3481 atggtcgcca ccggtctggt gcacgtggac ctggtcaaca agggcaacgc cgccaagttc
     3541 gacatacgct acaccgcggt ttatgccaac cagggcggtc aatggcgcct ggtgcacatc
     3601 cagaacggcc gtctgccggc caagacctga cattcctcct cacgaattgc ggacccgtga
     3661 aatgatcagg cagacaatcc gaagttggac acgcagtgaa ggccggcgga gggccgcggc
     3721 tttggttctc gtatgcctcg gcgttttggg aggcggctgt gccaccaacg agaagaaaga
     3781 accgacctac gcagagcaaa taatgaacag gccgctgccg gtaaacgagg cggagaagca
     3841 gcaggaatgc tgctggctgc ggggtgagat ggcgaggcag cgggcgcttg gcggatacgg
     3901 gaccgccatg caggatctcg ctactctgga gtcaaggtca tctcaagctg ggtgtgctgc
     3961 ggttcgggcc gacgtacagc cgggcgcgcc ggcggccggt ggcggcaact tcgacacgtg
     4021 ctacatgaac tgcaagcagt tcaccaacaa atccaacgac gtgtgcttcg accagtgctc
     4081 gaagtagcct aggctggctt tcgtcgcggg cctttcgcga ccgcctgcgc ggctacggcc
     4141 tgtacaggag cagtcaggaa tgaaagcccc gtcgacgctc gctattgcag cgcgccgaag
     4201 acgactttga tgttctgagt agagctcaca gagatctccc ccgagaggtc ggccgagatc
     4261 ggaaggatct cgagctttgc cccgcccccg gcattgc
//
LOCUS       DASPUL010000433         3758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1725560, whole genome shotgun sequence.
ACCESSION   DASPUL010000433 DASPUL010000000
VERSION     DASPUL010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1725560"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..406)
                     /locus_tag="VEJ00_10370"
     CDS             complement(272..406)
                     /locus_tag="VEJ00_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01609.1"
                     /translation="MAHLLREKGFNTFVIVGGLTAWRKAGQPLESVPKDDLVKLPTFS
                     "
     gene            complement(431..1243)
                     /locus_tag="VEJ00_10375"
     CDS             complement(431..1243)
                     /locus_tag="VEJ00_10375"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HXX01610.1"
                     /translation="MNGILLMTARHGYALTFAVLLAEALGLPFPAAIALVAAGAGVAS
                     HTLAGPAVLVAAIVGLLMGDIVQYWLGRSAGWALLGFLCRLSMNPETCILRSAESFYK
                     RGKVTLVIAKFIPGINTMAAPLAGSMKMRFPQFLRLDLAGALLYSITYLLVGYLSRDF
                     LAAVLNGFHAAGRAMEVLLIVAFVAYAIYRGVQFHKYRKYDVVPRVQVQELAARLASG
                     DGGRVLIVDVRSHGYYDVGAERIHGSIRIEPNNLDAEIQNLPKDKDIYLYCT"
     gene            complement(1867..2856)
                     /locus_tag="VEJ00_10380"
     CDS             complement(1867..2856)
                     /locus_tag="VEJ00_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXX01611.1"
                     /translation="MSATAAINGKEVFDLLRDDLIALENEFGRDTVSGVPAITEIGEY
                     LRAGGGKRIRPALLLLSAKLVNYAGQGAVRLGAVVEIIHTATLVHDDIIDEALTRRGR
                     PAANTQWGNSRCVLAGDWLYMQAFKIAVRERNFRILDTLIELTQQMVEGELLQMEKLG
                     RFITLEEYFDLIFRKTACLFSVCMRLGAILGGATAAQEEALASYGHDLGMAFQIVDDV
                     LDLTASEDVLGKPVASDLREGKVTMAVIHALERCTSEERAKIETILRDRAFNGVTHAQ
                     ILEILQRYGSVEAATARAASYAESAVAAICTFPDSEIKRALLWAPEYVVAREK"
     gene            complement(2942..3436)
                     /locus_tag="VEJ00_10385"
     CDS             complement(2942..3436)
                     /locus_tag="VEJ00_10385"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HXX01612.1"
                     /translation="MPDNTFDVVSKIDLQEVSNAIQQALKEIHTRFDLKDSKSNIELD
                     KDGIVLHSADEYKLKAVNDVLQQKLVKRGVPLKGLTYGALEPAAGGTTKRRITMQQGI
                     PIEKAREIVKTIKDSKKKAQASIQGDLVRVSSKDRDTLQEIIALLRGKDFGIDMQFTN
                     YRTN"
     gene            complement(3496..>3758)
                     /locus_tag="VEJ00_10390"
     CDS             complement(3496..>3758)
                     /locus_tag="VEJ00_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXX01613.1"
                     /translation="YPKAQQIHDAALEVPLDRLLIETDCPYLAPVPHRGKRNEPAFVK
                     ETARKLGELRGLSMDEVGEQTARNFYSFFKLAEMDESKVPAR"
BASE COUNT          768 a         1104 c         1105 g          781 t
ORIGIN      
        1 gctgcgatcc tggcgcacga agagcgcatc gtggagccgg ttccactgat gctcgggatt
       61 cgcatcgtag atggttactc gtgtctctga gtgaccgcgg tgggcaaaga ctgtcgcggc
      121 caggagggac atcccggcca cagcaggaag ggctacgcga aaaagccggc gcattgattc
      181 ctctccaggg attcagaagc cgacaccagg cactgtgccc tattttggca gaaagtcgcc
      241 tggggcctgg agaatgacat cgaaactgcg cctaggagaa cgtcggcagc ttcaccagat
      301 catctttcgg gaccgattcc agcggctggc cggccttgcg ccaggcggtc aggccgccga
      361 cgattacgaa cgtgttgaat cctttttccc gcagtaagtg cgccacccgg gcgctggtcg
      421 cttcgcgcgc tcaagtacag tagagataaa tgtccttgtc cttgggaagg ttctgaatct
      481 cagcgtcgag gttgttgggc tcgatacgga tcgagccatg gatgcgctcg gctccaacgt
      541 cgtagtaacc gtggctgcgc acgtcaacga tcagcacgcg gccgccatcc cccgaggcca
      601 ggcgggcggc aagttcctgg acttgaaccc gcggaacgac gtcgtacttg cgatatttgt
      661 gaaactggac gccacggtag atcgcatacg ccacgaaggc gacaatgagc agcacttcca
      721 tggcgcgccc ggcggcgtgg aatccattca aaaccgctgc tagaaaatcc cgagacagat
      781 atcccaccag caggtatgta atcgaataga gcagggcgcc cgccaaatcc aggcgaagaa
      841 actgcgggaa gcgcattttc atgctccccg ccaggggcgc ggccatggtg ttgatcccgg
      901 gaatgaactt cgcaatcacc agggtcacct ttccgcgctt atagaaggat tcggcagagc
      961 ggagaatgca ggtttccggg ttcatcgaca gccggcagag gaatcccagg agagcccagc
     1021 cggcggagcg tcccagccaa tattgcacaa tgtcgcccat caagagaccg acaatggccg
     1081 ccaccagcac tgcgggtccc gccaaggtgt gtgaagccac cccggcgcca gcggctacca
     1141 gggcaatcgc ggccgggaag ggcaggccga gagcttcggc gagcagcacg gcaaaggtca
     1201 gcgcatagcc gtggcgtgcc gtcatcaaga ggatgccatt cataactgat tcttgctacg
     1261 cagttccttg cccgcagctt gatcctgcga ttccccgtca acgatttcag attttgcaga
     1321 cgtttgattc gcctgctcag gaaagggttc agtataacca aaaagccggg cgcgtccgat
     1381 ggacaatcgc ccggcttggt aacggggagc gtgtcgttca ggaggacttt tccccgttca
     1441 gggtgagcaa ggcggcggcc cgcactcgga gtccttccgg ccctgccggg gccggcgcgt
     1501 cgaagaacca gggcgctttt cgttcaaggc ttcgactggg gtcgccgtct ctggctgatg
     1561 gcttgtctgc ggtggacagg accctcggcg acctgccttt ctcaagtgcc gaggggagag
     1621 gagtaaggcc gcttggtcac ggcccatcgc atgccagtgc gtctcgttag ccttccactc
     1681 ctttcagaca attccagcaa ccgggtgcat tttggaaaac tgcgcaggaa aaatctgtac
     1741 ctccggtcac ccatcatagt gacgaaggtc aagccagcct gggcgatggt tttgtgcgcc
     1801 ggctcacagg atctttcacc agatggcgag gggtttacgg cgtctggaac gccgaaagac
     1861 agaaggctac ttctcccgcg caacaacata ttccggagcc cacaacaggg cgcgcttgat
     1921 ctccgaatca gggaaggtgc aaatggccgc tactgcagac tcggcatagc ttgcggcccg
     1981 ggcggtggca gcttcaacgg agccgtagcg ttgcagaatt tccaggatct gcgcatgagt
     2041 gacgccgttg aacgccctgt cccgaaggat cgtctcgatc ttcgcccgtt cttcagaagt
     2101 gcagcgttcc agcgcgtgga tcacagccat ggtaactttg ccctcgcgca ggtcgctagc
     2161 cacaggtttg cccaggacat cctcggaggc ggtaaggtcg agcacgtcgt cgacgatctg
     2221 aaacgccata ccgagatcgt gtccatagct ggccagggcc tcttcctgcg cggcggtcgc
     2281 cccgccgaga atcgcgccca gccgcatgca tacagagaac aggcacgccg tcttacgaaa
     2341 gataaggtca aaatactctt cgagcgtgat gaagcggccg agcttctcca tttgcagaag
     2401 ttcgccctcc accatctgct gcgtgagttc aatgagcgtg tcaagaatgc ggaagttgcg
     2461 ctcgcgaacg gcgatcttga aggcctgcat gtagagccaa tcgccagcca gcacacacct
     2521 cgagttgccc cactgggtgt tggcggccgg tcggccacgg cgggtcaggg cctcgtcgat
     2581 aatgtcgtca tgcaccaggg tagcggtgtg aatgatctcg accacggctc ccagtcgcac
     2641 cgcaccctgg ccggcgtagt tcacgagttt cgcggagagc agcagcagcg ccggacggat
     2701 cctcttcccg ccccctgctc gcagatattc gccgatctcg gtaatcgcgg gaacgccgga
     2761 gactgtatcg cgcccgaact cattctccag cgcgatcaga tcgtcgcgca gcagatcgaa
     2821 gacttctttc ccattaatgg cggctgtcgc gctcatgttt tcagtattca gtacttagta
     2881 ctcagtaccc agtacccagg tgtcgcttcc gcgcagcaaa ctgagtactg ggtactgagt
     2941 actagttcgt acggtaattc gtaaactgca tatcgatgcc aaagtctttt ccgcgcagca
     3001 gggcaatgat ctcctgcagc gtgtcgcggt ccttgctgga gactcgcacc agatcgccct
     3061 ggatcgaggc ctgggctttc tttttcgaat ccttgatcgt ctttacgatc tcgcgcgcct
     3121 tctcgatcgg gatgccctgt tgcatggtaa tgcgtcgctt ggtcgtgccc ccggccgccg
     3181 gctccagcgc gccgtaggtc aagcctttca ggggaactcc tcgtttcacc agtttctgct
     3241 gcaggacatc gttgaccgcc ttcagcttgt actcgtcggc cgaatgcagg acgatcccgt
     3301 ccttgtcgag ttcaatgttc gacttcgaat ctttcagatc gaaacgggtg tggatctctt
     3361 tcagcgcctg ctggatggcg ttggagacct cctgcagatc gatcttgctc acgacgtcga
     3421 aggtgttatc gggcatacat ttctaatctt tctgaaatga tggaatcttt tacctgaggt
     3481 taacagaaag cggggctagc gcgcaggaac tttgctttca tccatttcag ccagtttgaa
     3541 aaaactgtag aaattgcgag cggtctgttc gccaacctca tccatcgaaa gcccgcgcaa
     3601 ttcgcccagt ttgcgggcag tttccttgac gaaggctggc tcgttgcgct tgccgcggtg
     3661 cggaaccggc gccagatacg ggcagtcggt ctcaatgagc aaacgatcca acggaacttc
     3721 gagggctgcg tcatgaatct gctgtgcctt gggataag
//
LOCUS       DASPUL010000434         7040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2001528, whole genome shotgun sequence.
ACCESSION   DASPUL010000434 DASPUL010000000
VERSION     DASPUL010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7040
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2001528"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1716)
                     /locus_tag="VEJ00_10395"
     CDS             complement(250..1716)
                     /locus_tag="VEJ00_10395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HXX01614.1"
                     /translation="MSKGLSSVDYAIIALYLAGITLFGLRFRKRHRSLRDYFLAGRDI
                     PWWAIALSIVAAETSTLTIISIPGLAYDTNFTFLQVVMGYVVGRVIISILLLPHYFRG
                     DLYTAYELIERRFGRGLRSLTAGLFLVTRAAAEGVRVYAVSIVVSIALGTGEVASIAI
                     ITALTLIYTFEGGLAAAIWTDVVQTVIYVGGTLVGIATILHLVPGGWTAIHAAAAGAG
                     KLQVFDFSRTVWNPYTFWSGIIGGAFFTVASHGTDQLIVQRLLAARGQKQSATALITS
                     GVAVMFQFALFLMVGILLWAYYQVPSVGFGKPDRIYPTFIVTRMPHGISGLLIAAVLA
                     AAMSNLSAALNSLSSSAILDFYVRLRPQVDEHKKMRLSRLATVGWALVLFGLAMIALH
                     RAGRVVEVGLQIASVAYGALLGAFLLGVLTRRANQRGAIVGMVCGFGVELYLWLGTRV
                     PWTWWVMIGSAVTFGVGWVASMGFGLKEGIPQESGQAT"
     gene            1974..2924
                     /locus_tag="VEJ00_10400"
     CDS             1974..2924
                     /locus_tag="VEJ00_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HXX01615.1"
                     /translation="MSYYLGIDGGGTKTTCAVGDETKLLARATAGPSNIVRVGEAQAR
                     ASLQESVRQACAAAGITPGQVVHTCAGGSGAARPELAAVVRRALAEILPTPVDVVGDI
                     QIALEAAFDTGPGVIVIGGTGSIAYGRNSQGNTARAGGWGFAISDEGSAHWIGRSAVS
                     AVLRAADLTDEGSEARIRLQNSSIVAALFKSWGVNSFADLARAANSIPPPDFATLFPP
                     VAGSGDDLGAQVLVRAGRELAQLGVVVIRRLFAGGDAATVPVAMIGGVFRHAPQVREV
                     FYNELRVIDARVEVNPQVVEPVEGALRLARRAARKVARAE"
     gene            2980..3723
                     /locus_tag="VEJ00_10405"
     CDS             2980..3723
                     /locus_tag="VEJ00_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01616.1"
                     /translation="MTDIEPEADQTPAALVRGSGPEGEAALTGDLALALLENRDLAPE
                     AVEEITRNPALMKSRKVRLAVAAHPRTPRRIALRLIRELYTFDLMRLSLTPAVAADLK
                     RLADELLVSRLASITLGERISLARRSSSLVAAGLLLDKEPRVWQTALENPRLTEAAIV
                     KALQRPNATAAFVDAVCHHPKWSLRHEIRMALLRNAHTPLARAIEFARRLPPPVLRDI
                     LHASRLPEKIKEYLRKDLPRGFASSKRSC"
     gene            complement(3742..5601)
                     /locus_tag="VEJ00_10410"
     CDS             complement(3742..5601)
                     /locus_tag="VEJ00_10410"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HXX01617.1"
                     /translation="MKVRQWITAIVLLLLVAAAIVAWLSTRPLPAPSQETAAAPGKKQ
                     IPQKAQATAHPLVDQRPLETARRMAALASTPEEQALAHEAEKVGDHEVDLAFFDALRT
                     AEENPPPLSPEAKQIAARKAKAEQTLKEDQENITLLTRKVAAAPESQKDNLQDQLDVA
                     KAQADLDQDELDDATEDLAQAGGDPQAKIKRLQAEHEAGDHNVAAAGSAVNPHEQDYQ
                     AHTLLSIFRAWQALRDKEAQLQAAHNETVEKQAYLSKRHAELEAQIGKDKENREAAKQ
                     QAKGFSKGSKAASRDESKSAAKSAIDALKQYTMDQKNLADRGRRLQDEQELADVYTNW
                     IALVAARERAALHNLIESVLWILLVLLAVYLVDRLIERLFTGVTAENKRIDTLRAVVK
                     FAAQALGAIAILFIVFGMPTQTTTVLGLAGAGLTVAMKDFIVAFFGWFILMGRNGIRV
                     GDWVEIQGVAGEVVEVGLLKTVLLETGSWTDAGHPTGRRVSFVNSFAIEGHFFNFTTS
                     GQWMWDELTILIPSSQDPYPVIDGLRKLVEKETKANAEKAETEWRQANARYRARALSA
                     EPGINVRPTGSGVEVRARYITRAYERHETRKRLYEAIVQMMHGKREETAGARD"
     gene            5721..6266
                     /locus_tag="VEJ00_10415"
     CDS             5721..6266
                     /locus_tag="VEJ00_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01618.1"
                     /translation="MLESLRSILEEPWKPVSRAAVIAWLVFYVAFLTYAFSAHGGFLF
                     IDNANLVVHEGGHNLFGWFGSTLGLWGGTLLQWLVPFLLAAYFFSQRQTTGFVFCLFF
                     FFENWLYTATYMADARAQVLPLVTTGDPDFVEHDWYAIFSSLGVLDWDTKIAAVVRIL
                     GWGGMLGCAAWLAARTKAQGE"
     gene            complement(6331..>7040)
                     /locus_tag="VEJ00_10420"
     CDS             complement(6331..>7040)
                     /locus_tag="VEJ00_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01619.1"
                     /translation="PRPDFSKGYVWAVYSGYDIFTANPDGSNLKQLTHTPGYDAEATI
                     SLDGKKITFTSMRRGDLDIYSMDADGRNVKQLTHELGYDGGPTFSPDRKWIVYRAYHP
                     QTEKEISDYKELLAQNLIRPTSLEIWIMKADGSGKKQITNLGAASFGPAFTADGKKII
                     FSSNYDPETHATGGMGNFELWLINPDGSGLERVTYSDGFDGFPMFSPDGKKLVWASNR
                     NAKAPHETNIFIADWVP"
BASE COUNT         1288 a         2306 c         2037 g         1409 t
ORIGIN      
        1 tggggtgtga tgtccctgcg agtcaagagg gtaatctcag ccgccaatct acctcggtag
       61 taggggaagg cggtggctcg cttcaagaaa aattccaccg tcttcccgaa acgaaacaaa
      121 agctcttacc gcaaagaacg ctaagaaaag gccgcaaagg tcgcaaagaa aatcgaccac
      181 gacgacgctc actaatttct tggcgttctt tgcggccttt tcttcgcgag ctttgcggta
      241 aagatgtgtt cacgtcgcct gtccagattc ctggggaata ccctccttga gcccgaaccc
      301 catgctcgcc acccacccca ccccgaacgt caccgcgctc ccgatcatca cccaccaggt
      361 ccacggaacc cgcgtcccca gccacaaata tagttccacc ccaaacccgc acaccatccc
      421 cacgatcgcg cctcgttgat tcgcccgccg cgtcagcacg cccagcagga acgcccccag
      481 tagggccccg tacgccacgg acgcgatctg cagccccact tccaccacgc gtccggcgcg
      541 atgcagcgcg atcatcgcca acccaaacag caccagtgcc cagccgactg tcgccagccg
      601 cgacagccgc atcttcttgt gctcatccac ctgcggccgc agccgcacgt agaaatccag
      661 gatcgcgctc gacgacagcg aattcagcgc cgcgcttagg ttcgacatcg cggctgccag
      721 gactgccgca atcagcagcc cggaaatgcc gtgcggcatc ctggtgacga taaacgtcgg
      781 ataaatccgg tccggcttgc cgaatcccac cgacggcacc tggtagtagg cccacagcag
      841 aatccccacc ataagaaaga gcgcgaactg aaacatcaca gccactccgc tcgtaatcag
      901 agccgtagcc gactgcttct gcccgcgcgc cgctagcagc cgctgcacga tgagttgatc
      961 ggtgccgtga ctcgcgaccg tgaagaatgc cccgccgata attccagacc agaacgtata
     1021 aggattccag acggtgcggc tgaagtcgaa cacctgcagc ttgcccgcac cggctgccgc
     1081 ggcgtgaatc gccgtccagc ccccgggcac aaggtgcagg atcgtcgcga tccccaccag
     1141 cgtgccgccg acatagatca ccgtctgcac cacgtcggtc cagatcgccg ccgccagccc
     1201 gccctcaaac gtgtagatca acgtcagcgc agtgatgatc gcgatcgacg ccacttcgcc
     1261 cgttcccagc gcaatcgaga ccacgatcga aaccgcgtac acacgcacgc cctctgccgc
     1321 ggctcgcgtc accagaaaca atcctgcagt caaagaccgc agcccgcgcc cgaatcgtcg
     1381 ttcgatcagc tcataggctg tgtagagatc gccgcgaaag tagtgcggca aaagcagaat
     1441 gctgatgatc acgcgtccca ccacgtagcc catcactacc tgcaggaagg tgaaattcgt
     1501 gtcgtacgcc agcccgggga tgctgatgat ggtcagcgtg ctggtttcgg ccgctacgat
     1561 ggaaagagcg atggcccacc agggaatgtc gcgcccggcg agaaaataat cgcggagcga
     1621 gcggtgccgc ttgcggaagc gcagcccgaa cagcgtgatc cccgccagat acagcgcgat
     1681 gatggcgtaa tcaacagaac tcagcccctt ggacattggg tgcgctcact ctagaggttc
     1741 cttgatttcc ctgtcaattc aggacttgca ggccgtgcag cacgttcgac gcagccgccg
     1801 gccacgctgg aaagaaacgg gagaatccgt acaggatgtc cgtcttcgag ctgtccacta
     1861 ccttgccagt caacgcttgc agcgaatctc gcgtctggaa tcatcacccc gcagatttct
     1921 cttcagggag gtagtgcaac aacctggata cgcccctgtg ttagaactaa gccgtgtcct
     1981 actacctcgg cattgatggc ggaggcacca aaaccacgtg tgccgtgggc gacgaaacta
     2041 agctgctggc ccgggccaca gccggtccga gcaacatcgt gcgcgtgggc gaggcgcagg
     2101 cgcgcgcgtc gttgcaggaa tcggttcgcc aggcctgtgc cgctgcgggc attactcccg
     2161 ggcaagtcgt gcacacgtgc gccggaggct ccggcgctgc ccgcccggaa ttggcagcgg
     2221 ttgtccgccg cgcgctcgcg gaaatcctcc caacgccggt tgacgttgtc ggcgacatcc
     2281 agatagccct ggaagccgct ttcgatactg gccccggcgt gattgtcatt ggcggcacgg
     2341 gttccatcgc ctatggccgc aactcccagg gcaacaccgc ccgcgccggc ggctggggat
     2401 tcgccatctc cgacgaaggc tctgcgcact ggattggccg ctccgcggtc agcgctgttc
     2461 tgcgcgcggc cgatctcacc gacgaagggt ccgaggcccg catccgcttg cagaactcgt
     2521 caatcgtggc ggctcttttc aaatcctggg gtgtgaactc gttcgccgat ctcgcccgcg
     2581 ccgccaactc gattcccccg cctgacttcg cgacgctgtt tcctcccgtc gccggcagtg
     2641 gggacgatct gggcgcacag gttcttgttc gagccggcag ggaactggct cagttggggg
     2701 tcgttgtgat ccggcgcctg ttcgctggcg gcgacgctgc caccgtcccg gtggccatga
     2761 tcggaggcgt ctttcgtcat gctccgcagg tgcgcgaggt tttctacaat gagctgcgcg
     2821 tgatcgacgc ccgcgtggaa gtcaacccgc aggtcgtcga accggtggaa ggcgccttgc
     2881 gcctagcgag acgagcggca cgaaaagtcg ccagggcaga gtagaggtta agaatttccg
     2941 ccgacgagac gcaccagccg tgcagagccg ttgccccgga tgacagacat cgaacccgaa
     3001 gccgatcaaa ccccggcagc gttggtgcgc ggctccggtc ccgagggtga agccgctctc
     3061 accggagact tggccctcgc ccttctcgaa aaccgcgacc tcgcccccga agctgttgaa
     3121 gagatcacga ggaatccggc cttgatgaag tcgcgcaagg tgcggctagc tgtggccgcc
     3181 catccacgca cgccgcgtcg catcgcgctg cgcctgatcc gcgaactcta cacgttcgat
     3241 ctcatgcggc tctcgctgac gcccgccgtc gctgccgacc tgaaacgcct cgccgacgag
     3301 ttgctggtct cccgtctggc gtcaattacg cttggcgagc gtatctcgct ggcacgccga
     3361 tcctcatcgc tggtggcggc cggtctgctg cttgataaag aaccgcgggt ttggcaaacg
     3421 gcgctcgaga atccacgcct gaccgaggcc gccatcgtaa aggcattgca gcgtcccaac
     3481 gcaactgcgg ccttcgtcga tgccgtctgc catcacccaa agtggtcgct gcgtcacgag
     3541 atccggatgg cccttctgcg gaacgcccac accccgctgg cccgcgccat cgagttcgcc
     3601 cggcgcctcc cgcccccggt actgcgcgac atcctgcacg cctcgcggct gccggagaag
     3661 atcaaagagt atttgcggaa agacctgccg cgaggatttg cttcttcaaa gaggagttgt
     3721 taacgcttga acgactgatc cctaatccct ggcccctgcc gtttcttccc gcttgccgtg
     3781 catcatctgc acgatggcct cgtacaggcg cttgcgggtt tcatgccgct cgtaggcgcg
     3841 cgtaatgtag cgggcgcgaa cttccacccc ggagccggtc ggccgcacgt tgatccccgg
     3901 ctcggccgac agcgcccgcg cccggtaacg cgcgttggct tgccgccatt ccgtttccgc
     3961 cttttccgca ttggccttgg tctctttctc gaccagcttg cgcaagccgt cgatcacagg
     4021 atacgggtcc tggctcgatg gaatcagaat cgtcagctca tcccacatcc actgccctga
     4081 ggttgtgaag ttgaagaagt ggccttcgat ggcgaagctg ttcacgaacg aaacccgccg
     4141 ccccgtagga tgcccggcgt cggtccagct gccggtttcg agcaacacgg tcttgagcag
     4201 tcccacctcg actacttcgc cggcgacgcc ctggatctcc acccagtcgc cgacgcgaat
     4261 cccgttgcgc cccatcagaa tgaaccatcc gaagaaggcg acgatgaaat ctttcatcgc
     4321 caccgtcagg cccgcgccgg ccaggccgag aacggtagtc gtctgcgtgg gcatgccgaa
     4381 gacgatgaac agaatcgcga tcgcgcccaa tgcctgcgct gcaaatttca ctacggcccg
     4441 cagcgtatca atgcgcttgt tctcggcggt tacgccggtg aacaggcgct cgatcagcct
     4501 gtccaccaga tataccgcga gcagcaccag cagaatccac agaaccgact caatcagatt
     4561 gtgcagcgca gcgcgttcgc gagccgcgac cagcgcgatc cagttggtgt agacgtcggc
     4621 aagttcctgc tcatcctgca gccgccgtcc gcggtcggct agatttttct ggtccatcgt
     4681 gtactgcttc agagcatcaa tcgcggattt ggctgccgac ttcgactcgt cacggctcgc
     4741 cgccttgctg cctttggaga accctttggc ctgctgcttc gcggcttcgc gattttcttt
     4801 atccttcccg atctgcgctt ccagttcggc gtgccgcttg ctcaggtagg cttgtttttc
     4861 gacagtctca ttgtgggcgg cctgcaactg agcttctttg tcgcgcagcg cctgccatgc
     4921 gcggaagatg ctcagcagcg tgtgcgcctg gtagtcctgc tcgtgcgggt tcaccgccga
     4981 tccggcggcg gccacgttgt gatcgccggc ttcgtgctcg gcctgcagcc gcttgatctt
     5041 cgcttgcgga tctcccccgg cctgcgccag gtcttcggta gcgtcgtcca gttcgtcctg
     5101 atccaggtcg gcttgcgcct tggcgacgtc aagctgatcc tgcaggttgt ctttttgcga
     5161 ctccggcgcg gcagccacct tccgcgtcag cagcgtgatg ttttcctgat cttccttcag
     5221 cgtctgctcc gccttggcct tgcgggcggc gatctgtttg gcttcgggcg agagcggagg
     5281 aggattctct tccgctgtgc gcagcgcgtc gaaaaaagcg agatcgactt cgtgatcccc
     5341 gaccttttcg gcctcgtgag ccagcgcttg ttcttcggga gtgctggcca atgcggccat
     5401 gcgccgcgcg gtctccagcg ggcgctgatc gaccagcgga tgcgccgttg cctgtgcttt
     5461 ctgcggtatc tgtttcttac ctggagccgc ggccgtctcc tgggatggcg cgggcagcgg
     5521 ccgcgtcgag agccacgcca cgatggccgc ggccaccagc agcaacagca caatcgcggt
     5581 aatccactgg cgaactttca taatcggccg acggttgttg agaccactgt aacaagaaat
     5641 ggccagcgct tgcgatgccg cacgtcggta atctgccctg cgacggctcc tgctcctaga
     5701 atcgtcgcag ctgcatcctt atgctcgaat ccctgcggtc gattcttgag gagccctgga
     5761 agcctgtttc ccgcgcggct gtgatcgcgt ggcttgtctt ctacgttgcg ttcctcacct
     5821 acgctttctc cgcacacgga ggatttctct ttatcgataa cgctaacctg gtcgtgcacg
     5881 agggcggaca caacctgttc ggctggttcg gttctacgct tggactttgg ggaggcacgc
     5941 tcttgcaatg gctggtgccg ttccttctgg ctgcgtattt cttcagccag cgccagacaa
     6001 ccggcttcgt tttctgcctg tttttcttct ttgagaactg gctctacacc gcaacctata
     6061 tggcggacgc ccgcgcccag gttctgccgc tggtcacgac tggcgatccg gatttcgtgg
     6121 agcacgactg gtacgcaatc ttttcgagcc tgggtgttct tgattgggac acgaagatcg
     6181 cggcggtggt gagaatactg ggatggggtg ggatgctggg atgcgcggcg tggctggccg
     6241 caaggacaaa agcccaaggc gagtgactct tcaatcggcg atcgcaaatc ggtagtcgcc
     6301 aattcaacgg tcgacaatca acagtttccc ctacggcacc caatccgcaa tgaaaatatt
     6361 cgtctcatgc ggcgccttgg cgttgcgatt cgacgcccac accagcttct tgccgtcggg
     6421 cgagaacatg gggaagccat caaagccgtc gctgtaggta acgcgctcca gcccgctgcc
     6481 atcaggattg atcagccaca gctcgaaatt gcccatgccg cccgtggcgt gcgtctctgg
     6541 atcatagttc gaggagaaaa tgatcttctt gccgtcggcc gtaaacgccg gaccgaagct
     6601 ggcggcaccc aggtttgtaa tctgcttctt gccgctgccg tcagctttca tgatccagat
     6661 ttcgagcgac gtcggacgaa tcaggttctg cgccagcagt tccttgtagt cggagatttc
     6721 cttttccgtt tgcggatgat aggcgcgata gacgatccac ttgcggtcgg gcgagaacgt
     6781 cggcccgccg tcgtagccaa gctcgtgggt gagctgcttc acattcctgc cgtcggcgtc
     6841 catcgagtag atgtcgagat cgccacggcg catagaagtg aacgtgattt tcttgccgtc
     6901 aagcgagata gtcgcctcgg cgtcgtatcc gggagtgtgg gttagctgct tcaggttcga
     6961 gccgtcggga ttcgccgtga aaatgtcgta tcccgagtac actgcccaca cgtagccctt
     7021 ggaaaaatcc ggacgcggcg
//
LOCUS       DASPUL010000435         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1176701, whole genome shotgun sequence.
ACCESSION   DASPUL010000435 DASPUL010000000
VERSION     DASPUL010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176701"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VEJ00_10425"
     CDS             <1..498
                     /locus_tag="VEJ00_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013345880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HXX01620.1"
                     /translation="KSGRLRRWINFYPPFLGAGICVKRIAPDMKAIDVELKLRFWNAN
                     YVGTHFGGSLFAMTDPFYMLMLMSNLGRDYIVWDKAASIRYRKPGRGTVRAEFRLTDA
                     QLDDIREKLKTLPKYEPVLNVEVKDEQGTVIAEVEKVIHVRRKDPAQPGNGQPRSGTD
                     VQPTV"
     gene            545..1180
                     /locus_tag="VEJ00_10430"
     CDS             545..1180
                     /locus_tag="VEJ00_10430"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HXX01621.1"
                     /translation="MYIPEHFRVRDHAAALAFMRANPFAILVSSTEDGPFATHLPLVV
                     RESGGQVVTRGHVAKANPHWRYLEQQPQCLVIFHGPHAYISPKNYTTREVVPTWNFGT
                     VHLYGNARVFSAPDELLGMLHELIPMFEPAYAEQWASLSQAYRERMLSHIVGFEIAVT
                     RIEAKFKLSQNRTREEQENVITSLESASDTVVSGTARLMREQGLGLKKERS"
     gene            1273..2148
                     /locus_tag="VEJ00_10435"
     CDS             1273..2148
                     /locus_tag="VEJ00_10435"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HXX01622.1"
                     /translation="MLVGRARHQAGALSAELRKLGATVLEIPFIEIRKPRSFLPLDSA
                     LTNLNTYDWLILTSVNGVEAMWDRMQRLRLHRLEKKRDDADFCRAELLLQPWKSGPSR
                     PRQAANSARALAPGARLRIAAIGPATKKAIEQRGVKVDVVPKEYVAESVVRSLKRKVK
                     GKRVLLVRAKVARDVIPRELRGAGAHVDVVEAYETVVPQSSRRRLQALLEDPRRRPHV
                     VTFTSSSTVRNFVKLLGARRGLSRKLAGAQMASIGPVTSSTLRDSGLPVDVQARNFTI
                     PGLVEAIGQTVAARR"
     gene            complement(2157..>3950)
                     /locus_tag="VEJ00_10440"
     CDS             complement(2157..>3950)
                     /locus_tag="VEJ00_10440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXX01623.1"
                     /translation="TWSPDGKTVAFISNMSGRNNIWVVSADGGWPVQLTISDQRQTAP
                     AWSPDGNWIAYQSDNDGDEQWDIFLVSPKTGKVVNLTQTREIAESNPTWSADGRFLAY
                     EVKPKTSAASEIDVYDTVLRELKHITSNTPQDKSNTNPIWSKDGKYIVYTQEQAKGTD
                     SNIFIADVATGKSTLLTPHAGELQFSASDVSPDGKQVLLTSNAANGCENVGLLSVETK
                     KITWLTSDTWEIRGRNFSPDVKYVTFTANVDGNEDIYLYDLATRKSTTLPAAKGLNEL
                     GGKPSAFTSDSSRLLYYHNGPTAPGDLWVYDLATGKSQQITHSLVGGVRPEDMVEPIL
                     AHYPSRDGKWTISAFLYVPYNMARNGQNAAIVYIHGGPAAQSTNSFNRFIQFAVNQGY
                     MVLAPNYRGSTGFGKEFEQANLFDMGGGDLQDVLAGVDFIKQTGHLDPKKIAVMGASY
                     GGYLSMMAVTKASDVWAAGVPIVPFVNWFTEIQNEDPELRESDLATMGDPAKNKALYE
                     DRSPINFVDQVKAPLMLLAGGHDPRCPKSETQQVVDAIKKRGGAVDFKVYENEGHGFA
                     RVENQIDAYKRVADFLLAHVVPADCACTVTE"
BASE COUNT          773 a         1241 c         1139 g          797 t
ORIGIN      
        1 aagtcgggcc gcctccgccg ctggatcaac ttctatccgc ccttcctcgg cgccggcatt
       61 tgcgttaaac gcatcgcccc tgacatgaaa gccatcgacg tcgaactgaa gctccgcttc
      121 tggaacgcca actacgtggg cacgcacttc ggaggatcgc tctttgccat gaccgaccct
      181 ttctatatgc tcatgctgat gtccaaccta ggccgcgact acatcgtctg ggacaaagcc
      241 gccagcattc gctatcgcaa acccgggcgc ggcacggtac gcgccgagtt tcgcctgacc
      301 gacgcgcaac ttgacgacat tcgtgagaag ctgaagacgc tgccgaaata tgaaccggta
      361 ctcaacgtcg aagtcaaaga cgagcaggga accgtgatcg ccgaagtgga gaaagtcatc
      421 cacgtacgtc ggaaagaccc agcgcagccc ggaaatggcc agccgcgaag cggcacagac
      481 gtgcagccca ccgtgtaggc gatgggggta gttgaagatc agccctgagg gggcgaagag
      541 aatcgtgtac attcctgaac atttccgcgt gcgcgaccat gccgccgcgc tggcgttcat
      601 gcgcgcgaac ccctttgcca ttcttgtctc cagcacggag gatggcccgt tcgctaccca
      661 cctgccgctg gtcgttcgcg agagcggagg acaagtcgtc acccgcggcc acgttgccaa
      721 ggccaaccca cactggcgct accttgagca gcaaccgcaa tgcctggtca tcttccacgg
      781 cccgcatgcc tatatatcgc caaagaacta caccacgcgc gaggtcgttc ccacctggaa
      841 cttcggaaca gtccaccttt atggcaacgc gcgcgtgttt tccgcgccgg acgagctgct
      901 cggcatgctg cacgaactca tccctatgtt cgagccggcg tacgctgagc agtgggccag
      961 cctgagccag gcttaccgcg aacgcatgct gagccatatc gtcggcttcg agatcgctgt
     1021 caccaggatc gaagcgaagt tcaaactaag ccagaatcgc accagggagg aacaggagaa
     1081 cgtcatcacg tccttggaat cagcctcaga tactgtcgtg tccggaaccg cgcgcctgat
     1141 gcgcgaacaa ggtctgggcc tcaagaagga gcgcagctaa ggccgtgtgg ggcgggcgct
     1201 cccgcccgcc aactccaaac gatgagcgag ccctccaact cgaagcccac ccagcctcta
     1261 caaggaatcc gtgtgctcgt cggccgcgct cgtcaccagg cgggagccct gtcggccgag
     1321 ctgcgcaagc tcggagcaac ggttctcgag atcccgttca tcgagatacg caaaccgcgc
     1381 tcgttcctcc cgctggattc cgccttgacg aatctcaaca cgtacgactg gctcatcctg
     1441 actagcgtga atggagtcga ggcgatgtgg gaccgcatgc agagacttcg gctgcatcga
     1501 ttggaaaaga aacgggacga cgccgacttt tgtcgcgccg agctgctgtt gcaaccgtgg
     1561 aagagcggcc cttcacggcc acgtcaagcg gccaactcgg ctcgggcttt agcccctggg
     1621 gcccgtctgc gcatcgccgc tattggtccg gcgacgaaga aagccatcga acaacgcggc
     1681 gtcaaggtcg acgtcgtgcc caaggaatat gtagccgagt ccgtcgttcg cagcctgaag
     1741 aggaaagtga aggggaaacg cgtgttactc gtccgtgcca aggtcgcccg cgacgtgatt
     1801 ccgcgcgaac tgcgaggagc cggtgcgcac gtcgacgtag tcgaagctta cgaaaccgtt
     1861 gtgccccaga gttcccggcg ccgattgcag gctctgctcg aggatccgag acgccgcccc
     1921 cacgttgtca cattcaccag ctcgtcgacc gtgaggaact tcgtgaagtt gctcggtgcg
     1981 cgacgtggct tgtccaggaa gctcgccggc gcgcagatgg cctcgatcgg acctgttact
     2041 tcttcaaccc tgcgcgattc gggcctgccg gttgatgttc aggcgcggaa ttttactatc
     2101 cctgggttgg tggaagcgat tggccagacg gtcgctgccc gaagatgagg tatctcttac
     2161 tctgtcacag tgcacgcgca atcggcgggc acaacgtgcg ccagcaggaa atccgccacg
     2221 cgtttgtagg cgtcgatctg attctcgacg cgcgcgaagc cgtggccttc attctcgtag
     2281 accttgaagt ccacagcacc gccccgtttc ttgatggcat cgaccacctg ctgggtctct
     2341 gacttcggac agcgcggatc gtgtcctccg gccagcagca tcagcggcgc cttgacctga
     2401 tcgacgaaat tgattggcga gcggtcttca tacagcgcct tgttcttcgc cggatcgccc
     2461 atggtcgcca gatccgactc gcgcagctcc ggatcttcat tctggatttc cgtgaaccag
     2521 ttcacgaagg ggacaatggg cacgcccgcc gcccagacgt ccgacgcttt ggtcaccgcc
     2581 atcatgctca gatacccgcc ataactcgcg cccatcacgg cgatcttctt gggatcgaga
     2641 tgcccggtct gcttgatgaa gtcgacgccc gccagcacat cctgcaagtc gccgccgccc
     2701 atgtcgaaca ggttggcctg ctcgaactct ttgccgaagc ccgttgaccc gcgatagttc
     2761 ggagccagca ccatgtatcc ctgattcacc gcgaactgga taaatcggtt gaaggaatta
     2821 gtgctctgcg ccgcaggtcc gccgtggatg tacacgatgg ccgcgttctg cccgttgcgc
     2881 gccatgttgt aaggcacgta gaggaatgcg gagatcgtcc acttgccgtc gcggctggga
     2941 tagtgcgcca gaattggctc aaccatatct tcgggccgca cgcccccgac gagcgaatgc
     3001 gtgatctgct gcgacttccc agtggcgagg tcatacaccc agagatcgcc cggcgccgtc
     3061 ggcccgttgt ggtagtaaag cagccgtgag ctgtcagatg tgaatgcgga gggttttccc
     3121 cccagctcgt tcagtccttt cgcggcgggc aaagtggtgg acttgcgggt ggcgagatcg
     3181 tagaggtaga tgtcctcgtt gccgtcaacg tttgcggtga acgtgacata tttgacgtcg
     3241 ggcgaaaaat tccggccgcg gatttcccac gtgtcgctgg taagccaggt gatcttcttg
     3301 gtctcaacgc taagcaagcc cacgttctcg catccgttgg cagcgttgga agtcagcaga
     3361 acttgttttc cgtctggaga aacgtcgctc gctgaaaact gcagctcgcc tgcatgcggc
     3421 gtcagcagcg tgctctttcc cgtcgcaacg tctgcgatga agatattcga atccgtcccc
     3481 ttcgcctgct cctgggtgta gacgatatac ttcccgtcct tcgaccagat cggattcgta
     3541 ttgctcttat cctgcggggt gttcgaggta atgtgcttca gttcgcgcag caccgtgtca
     3601 tacacgtcaa tctcagaagc cgccgaggtc ttcggcttga cttcatacgc caggaaccgg
     3661 ccatccgccg accatgtcgg attcgattcc gcgatctccc gcgtctgcgt caggtttacg
     3721 acttttccgg tcttgggcga gaccaggaaa atatcccact gctcatcacc gtcgttgtcg
     3781 gattgatagg cgatccagtt tccgtctggc gaccaggcgg gcgcggtctg cctctgatcg
     3841 ctgatcgtga gctgcaccgg ccatccaccg tcggccgaaa ccacccagat attgttccgc
     3901 ccgctcatgt ttgaaatgaa cgccaccgtc ttgccgtcgg gcgaccacgt
//
LOCUS       DASPUL010000436         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_490294, whole genome shotgun sequence.
ACCESSION   DASPUL010000436 DASPUL010000000
VERSION     DASPUL010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490294"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VEJ00_10445"
     CDS             <1..1294
                     /locus_tag="VEJ00_10445"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXX01624.1"
                     /translation="ARPATAEDGMSLTVALALLLLLGLLTLVSYADRVYQEIGKFLSR
                     DFQDNIDVFEQRVEPNLKVSRTRASLSMAVLTQLTMAAIALLVGFVVFSDQLWSVVEI
                     LQATISLVLIVVVCNRFLPFLFFSRTKGEWLVRWTLTLRLLIYLVMPVTIVLGFLQSV
                     VSLTKQSTTEQPETSAEAVDALIEAGQEEGIIQEGDRDLIQSVVEFSGKTVREAMKPR
                     PAMFAVPTTTTVERFIEMLGAKHYSRVPVYEGSVHNIKGIVYAQDVLQVPDTEAHVRT
                     LDTLMRRDVYYVPESKLGSDLLREMQKQNIRMAIVVDEYGGVAGLVTIEDLVEEIVGE
                     IRDEHDKPEIVREGERSFIVSGGMDIDRLDELFGTKPEGKESATIAGLVSELAGRIPH
                     KGETVEEDGLRFEVLESTDRKIERVRVTAVQPQQLKLI"
     gene            1382..2368
                     /gene="era"
                     /locus_tag="VEJ00_10450"
     CDS             1382..2368
                     /gene="era"
                     /locus_tag="VEJ00_10450"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HXX01625.1"
                     /translation="MSFRSGFVCILGRPNAGKSTLLNALVGEKLAIISPKPQTTRNRI
                     LGIVHLPKGKGQTRDGGQIVLIDTPGVHKPDSSLGRKMMVEVREAMEGCDLVLLIIDV
                     THKLDARDQFAIDLLKQSGTKAFLLLNKIDLIREKSKLLPLIEEYRKFHNFREVIPIS
                     ALKRSGLDTLLDEIIAALPAGPAYFPEDEITDQPARFMAAEIIREQVLLNTSEEIPYA
                     TTVVVDSFEEGAKLTRIAATIYCERQGQKGILVGKRGQMLKKIGTAARLQIERMLGTR
                     IFLELYVRVEPGWRDSRRFVEELDWRRQLEHRMIEQEAGASNAPSRQNDTSD"
     gene            2470..>2903
                     /locus_tag="VEJ00_10455"
     CDS             2470..>2903
                     /locus_tag="VEJ00_10455"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXX01626.1"
                     /translation="MVPGDSSEEHFPEYDFDPAKLSLRVRVTLAADRKAVDPVVEQVM
                     QAVRQMKGVNGKEDAIELALAEALANAVVHGAKEDPRKTVECLVMGDEQRGILIIVRD
                     PGEGFDPNSIPACTMGENVYSNHGRGIFLINQLMDEVKFRKNG"
BASE COUNT          624 a          863 c          867 g          549 t
ORIGIN      
        1 ggcgcgcccc gcgaccgcgg aggatggcat gagtctgacg gtcgccctcg cgctgcttct
       61 cctgctcgga ctgctgacgc tggtctccta cgcggatcgt gtgtatcagg agatcggcaa
      121 attcctgtcg cgcgacttcc aggacaacat tgacgtcttt gagcagcggg ttgagccgaa
      181 cctgaaagtc agccggactc gggcttcact ttcgatggcc gtgctgacgc agctgaccat
      241 ggcggccatc gcgttgctcg tgggcttcgt cgtcttcagc gatcagctct ggagcgtagt
      301 cgaaatcctg caggccacga ttagcctggt cctgattgtc gtggtctgca atcgctttct
      361 gccctttctg tttttttcgc gaaccaaggg cgaatggctg gtgcgttgga cgctcactct
      421 aaggctcctc atctacctgg tcatgccggt gacgatcgtg ctggggtttt tgcagtctgt
      481 cgtgtcgctg accaagcaaa gcacgacgga gcaacccgag acttccgctg aagctgtcga
      541 tgccctgatc gaagctggtc aggaagaggg gattatccag gaaggcgacc gcgacctgat
      601 ccagtcggtg gtggagttca gcggcaagac ggtgcgtgag gcgatgaagc cgcgccctgc
      661 catgttcgcc gtgcccacaa ccaccacggt cgaacgcttc atcgagatgt tgggcgccaa
      721 gcattattcg cgcgttcccg tgtatgaagg gagtgtgcac aacatcaaag gaatcgtcta
      781 cgcgcaggac gtgctgcagg tgcccgacac cgaagcccac gtgcgcacgc tcgatacgct
      841 catgcggcgc gatgtatatt acgtgcccga gagcaagctg ggcagcgact tgctgcgcga
      901 gatgcagaag caaaatatcc gcatggccat tgtcgtcgac gaatatggcg gcgtggccgg
      961 tctggtcacg atcgaggacc tggtggaaga aatcgtgggc gaaatccgcg acgagcacga
     1021 caagcccgag atcgtgcgcg aaggcgagcg ctcgttcatt gtttccggag gaatggacat
     1081 cgaccggctc gacgagctgt ttggcaccaa gcccgaaggt aaggagtcgg caacgattgc
     1141 cggcctggtc agcgaactcg ctggccgcat tcctcacaag ggcgagacgg tcgaggaaga
     1201 cggcctgcgc ttcgaggtcc tggaatccac cgaccgaaag attgaacgcg tacgcgtgac
     1261 tgcagtccag ccgcagcagt tgaaactgat ctagacggct ttcgaccctc ggctctcggt
     1321 ttccgccaaa gcgcggactc cggaagccga aagccggaag ccggaagccg aaagccgata
     1381 catgtctttc cgctccggat tcgtctgcat cctcggccgc cccaatgcgg gcaaatcgac
     1441 cttgctgaac gcgctggtgg gcgagaagct ggcgatcatt tcgcccaagc ctcagaccac
     1501 gcgcaaccgc atcctgggca tcgttcacct gccaaaaggc aagggccaaa ccagagacgg
     1561 cgggcagatc gtgctcatcg acactccggg cgtgcacaag cccgacagct ctctcggccg
     1621 caaaatgatg gtcgaagttc gcgaggccat ggaaggctgc gatctcgttc tcctcatcat
     1681 agatgtcacc cacaagctcg atgcgcgcga ccagtttgca atcgacctgt tgaaacagtc
     1741 gggcaccaag gctttcctcc ttctgaacaa gattgacctg atccgcgaga agtcgaagct
     1801 gctgcctctg atcgaggagt atcgtaagtt ccataacttc cgcgaggtga ttccaatttc
     1861 cgcgctcaag cgcagcggcc tggacacgtt attagacgag atcatcgccg ccctgcctgc
     1921 cggccccgca tattttcctg aagatgaaat caccgatcag cccgcgcgct tcatggcagc
     1981 ggaaatcatc cgcgagcagg tgctgctgaa taccagcgaa gagattccct atgcgactac
     2041 cgtcgtcgtc gacagctttg aagagggcgc gaagctgact cgaatcgcgg cgacgatcta
     2101 ctgcgagcgc cagggccaaa agggaattct cgtcggcaag cgcggccaga tgctcaagaa
     2161 aatcggcacc gccgcccgcc tgcagatcga gcgcatgctg ggaaccagga ttttcctcga
     2221 actgtacgta agagtcgagc cgggatggcg cgattcgcga cgctttgtgg aagaactcga
     2281 ctggcgccgc caactcgagc acagaatgat cgagcaggag gccggcgcga gcaacgcgcc
     2341 ctccagacaa aacgacacat cggattgaag ttgtgttgtc tcgagcatgc ccgttgctca
     2401 atccggggct ttgccgctag aatcgcatca ggccgcactc aaggcgcaag aggacgcaac
     2461 caacccacca tggtacctgg ggattcttct gaggaacact tcccggaata cgactttgat
     2521 cccgccaagc tcagcctgcg cgtgcgggtc acgctcgctg ccgaccgcaa ggccgtcgac
     2581 ccggtcgtcg agcaggtcat gcaagccgtg cgccagatga agggcgtcaa cggcaaggaa
     2641 gatgcgatcg aactggctct ggccgaagcg ctggccaacg ccgtcgtcca cggcgccaaa
     2701 gaagatcctc gaaagacggt tgagtgcctg gtgatgggcg acgagcaacg cggcatcctc
     2761 atcatcgtgc gtgatcccgg cgagggtttt gatcccaatt ccattcccgc ctgcaccatg
     2821 ggcgagaacg tctactccaa ccacggccgt ggaatcttcc tgatcaacca attgatggac
     2881 gaagtaaagt ttcgtaaaaa cgg
//
LOCUS       DASPUL010000437         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_631881, whole genome shotgun sequence.
ACCESSION   DASPUL010000437 DASPUL010000000
VERSION     DASPUL010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631881"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VEJ00_10460"
     CDS             <1..408
                     /locus_tag="VEJ00_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01627.1"
                     /translation="YWQIPLAHSLLTGYSDPTASASFRLVFGATVLVDLAFATVILVT
                     AIWFIRCRLSAANPYALSVFVLLVYRAMIPHLCRLGGGLGISIAAALGIGNMGTALFE
                     FLFVVPDLYPVVSVVLTLFLMQKYGTGFLPAHA"
     gene            complement(424..1266)
                     /locus_tag="VEJ00_10465"
     CDS             complement(424..1266)
                     /locus_tag="VEJ00_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXX01628.1"
                     /translation="MKRHIFWALLVCVALAVPVCTQQSSSNSSSSATSAQATPSQSAS
                     MPDLEPLPPHQAANFWDGDDPNLISLVTHPFANKKYVQRMTRPIKDRLEELDGLTTAN
                     SNAIKDVDARAQRGLQLASEKTSLADTHASDAVTRSQQAQTAASDASSRVSGAEQVVG
                     SLDRYKSGAQTEIRFRPGQTVLSKQAKDALDGMAAPLKGQHSYIIEVQGFAAGHGQAA
                     IASSQKMADSVVRYLVLTHQIPVYRIYVLSMGNTPLEGTAKRVSGGRVEVNVMKNDLV
                     TAQR"
     gene            complement(1497..2132)
                     /locus_tag="VEJ00_10470"
     CDS             complement(1497..2132)
                     /locus_tag="VEJ00_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXX01629.1"
                     /translation="MIHEILPVGPLQCNCSVIGDEATREAMVIDPGDDIDDVLALIRK
                     HNLQVKQIVITHAHIDHVGGAMKLRAATGAPILLNQNDYALLKMLDVQAAWIGMVSPG
                     KVEIDRSVTTGESVSVGSLTAQVLHTPGHTEGSICLYLPAEQKLIAGDTLFAGSIGRT
                     DLPGGSMQKIMRSLHGTVLALPDETVVVPGHGPLTTIGDERESNPFLVKNQ"
     gene            2300..>2574
                     /locus_tag="VEJ00_10475"
     CDS             2300..>2574
                     /locus_tag="VEJ00_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01630.1"
                     /translation="MAKYRQHGYQDRDWESKGKDSQKQERKPPPERKSGPPKWDHALG
                     PRPVNLPGTRAVSRCAQCGTVLQGMAADGKCPKCGFELHSCKQCMYF"
BASE COUNT          487 a          704 c          829 g          554 t
ORIGIN      
        1 tattggcaga ttcccctggc ccattccctc ttgaccgggt actccgaccc caccgcatcc
       61 gccagcttcc gtctggtatt cggagcgacg gtactcgtag atctcgcatt tgcgaccgtc
      121 attctagtaa ctgccatatg gttcatccgc tgcaggctgt ccgccgcaaa cccatacgca
      181 ctctcagtgt tcgtgcttct ggtttacaga gcgatgattc cccacctgtg ccggcttggc
      241 ggaggacttg gaattagcat cgccgcggcg ttgggaattg gcaatatggg aacagcgctt
      301 tttgaattcc tgttcgtggt ccccgatctc tatccggtcg tttccgtagt cttgacttta
      361 ttcctcatgc aaaagtacgg gaccgggttc ctcccagccc atgcgtgaag ggcttgccga
      421 accttatcgc tgagcggtca cgagatcgtt cttcatcacg ttcacttcga cgcgcccgcc
      481 gctgacccgc ttggctgttc cttccagcgg cgtgttaccc atgctcaaca catagatccg
      541 gtacaccggg atctgatggg tgagaaccag gtagcgcacg accgagtcgg ccatcttttg
      601 cgaactggcg atggcggcct gcccgtgacc ggcggcgaag ccctgcactt cgatgatgta
      661 gctgtgctgg cctttcaacg gggctgccat cccgtcgagc gcatctttgg cctgcttgct
      721 cagaaccgtc tggccggggc ggaaacgaat ctccgtttgc gcgccactct tgtatcggtc
      781 gaggctgccc accacctgct cagcgccaga aacgcggctt gatgcatcac tggcggcggt
      841 ctgtgcctgc tgggagcgtg tgacggcgtc actggcatgc gtgtcggcca gcgacgtctt
      901 ttcagaagcc agctggagtc cgcgctgggc gcgggcatcg acgtctttga tggcgttgct
      961 gttggccgtg gtcagcccat ccagttcttc gaggcggtct ttgatcgggc gcgtcatccg
     1021 ctgcacgtac ttcttgttgg caaaaggatg ggtgacgaga ctgatcaggt tgggatcatc
     1081 gccgtcccag aagttggcgg cctggtgggg tggcagaggc tccagatcag gcatgctggc
     1141 actttgcgag ggagttgcct gagccgatgt agcggaggaa ctggaattgg agctgctttg
     1201 ctgcgtgcaa acaggaacgg ccagggctac gcaaacgagc agcgcccaga aaatgtgacg
     1261 cttcatatga acctccttcg agcagggagc aggggtttga ctcccgcccg atgggcgagt
     1321 ggcgttacat cccaaaaaat tgcaggctta tagtacaccc attctggctt gcgaagatgt
     1381 gatttggcgc aggctgccat cgcagaaggc aaagatctga cccgaaaggc gccaggaaag
     1441 gccgctgagg gcacaaggta atacctgcct ggagccggcc gccgcctgtt cctagattat
     1501 tgattcttga ccagaaacgg gttgctctcg cgctcatcgc cgatggtcgt cagggggccg
     1561 tggccgggga cgaccacggt ttcgtcaggc agggcgagga cggtgccgtg cagggagcgc
     1621 atgattttct gcatggagcc tccgggtaga tcggtgcgac caatggagcc ggcaaacaag
     1681 gtatcgcccg cgatcaattt ttgttcggcg ggaagataga ggcagatgct gccttcggta
     1741 tggccggggg tatgcaggac ttgagcggtg agcgatccga ccgagacgga ttctccggtg
     1801 gtcacgctgc ggtcgatttc cacctttccc ggagaaacca tgccgatcca ggcggcctgc
     1861 acatcgagca ttttgagcag ggcgtaatcg ttctggttga gcaggatggg cgcgccggtg
     1921 gcggcgcgga gcttcatggc tcctccgaca tgatcgatgt gagcgtgggt gatgacgatc
     1981 tgtttaacct gaagattgtg cttgcggatc agggcaagaa cgtcgtcgat gtcatcgccg
     2041 gggtcgatga ccatggcttc gcgcgtggcc tcgtcgccga tcactgagca gttgcattgc
     2101 agcggaccga cgggaaggat ctcgtggatc atgggagtat tgtagcgagt atccagtacc
     2161 cagtactcag tactcagagt ttagactgat cccgctgaat aatgcgtact gagtactccg
     2221 tactgagtac cgacagcgcg gctcttgccg ggaagggcgg actgggtgga gaatacgggc
     2281 gcgtgcttgg gagggtagtg tggcgaaata ccgtcagcat ggatatcaag accgcgactg
     2341 ggaatccaag gggaaggatt cgcagaagca ggagcgaaaa ccgccgccag aacgcaagag
     2401 cggcccgccg aagtgggacc acgcgctggg cccacgcccg gtgaatttgc cggggacgcg
     2461 cgccgtgtca cggtgcgcgc agtgcggcac cgtgctgcag ggaatggcgg cggatgggaa
     2521 gtgtccgaaa tgcggtttcg aactgcattc gtgcaagcag tgcatgtatt ttga
//
LOCUS       DASPUL010000438         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_79595, whole genome shotgun sequence.
ACCESSION   DASPUL010000438 DASPUL010000000
VERSION     DASPUL010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79595"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VEJ00_10480"
     CDS             complement(<1..403)
                     /locus_tag="VEJ00_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01631.1"
                     /translation="MSNRSVTFPPRPSGWLAAWAAWLNPYRLRAHAIVLAICLWGAFA
                     VDFSTPGFFDRAGNIKFQDFLPLYVSARQIAQGRTNALYDQQAGVAAMKSIIGQANSS
                     RSMLSRLPNLYGPQVGLLFVPLARFSFTTAAW"
     gene            complement(400..993)
                     /locus_tag="VEJ00_10485"
     CDS             complement(400..993)
                     /locus_tag="VEJ00_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01632.1"
                     /translation="MRRKPILCLLCLLLASCSPRDFLTRRLAFDLLSASDAFKVRQQF
                     VLQTGILSNSAYASPEYLVLQHHGWISATNSPCSQGLAPPPCWGVLLTPSGVETIRAL
                     VSAEEASKPSFAIPVARRELLEITGISKQGNLADVEFIWKWAPLNEVGAALYSGELRY
                     KSTVGFRDYDDGWRLVQTSPHSGQTLDDALKNAELSP"
     gene            complement(1067..>2203)
                     /gene="amrS"
                     /locus_tag="VEJ00_10490"
     CDS             complement(1067..>2203)
                     /gene="amrS"
                     /locus_tag="VEJ00_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HXX01633.1"
                     /translation="AMPTLRDILDQRVREADASLYQTLENQRVRCFSCGHCCPIPQGQ
                     PGVCKVRYNRGGTLYVPWGYTAGMQCDPIEKKPFFHVHPGALAYSFGMMGCDLHCAYC
                     QNWVTSQALRDPEAVSPPLEVSPEHLVREAVEQGARVVVSTYNEPLITSEWAVAIFKE
                     AKAAGLLTGFVSNGNATPQVLDYLRPWLDLYKVDLKSFDDRRYHELGGRIGPILDSIR
                     RIHQMGLWLEIVTLLIPGFNDSADELRRMAEFLASISPDIPWHVTAFHADYKMTDQRD
                     TSVDDLLRAAEIGQQAGLRYIYAGNLPGRVGQWEDTRCPQCDETLISRHGYLIEDYRL
                     TDEGRCSACAAQIPGRWAAKFDGQIASHPFLPRSRSRLFTITGR"
BASE COUNT          479 a          602 c          731 g          391 t
ORIGIN      
        1 tccacgcggc ggtagtgaaa gagaatcgcg ctagaggtac gaagagaagc ccgacctgcg
       61 gtccgtagag gttgggcagg cgcgaaagca tcgagcgcga cgaattcgcc tgtccgatga
      121 tcgacttcat cgcagccacg cccgcctgct gatcgtagag agcattggtg cggccttgcg
      181 caatttgtcg agccgaaacg tagaggggaa gaaagtcctg aaacttgatg ttcccggccc
      241 gatcaaagaa gccgggagtg gagaaatcaa cggcgaaggc tccccacagg caaatggcaa
      301 gcacgatcgc gtgtgcgcgc aggcggtagg gattgagcca cgctgcccac gccgccagcc
      361 agccgctggg tcgtggcggg aaggttacag agcggttgct cacggggaaa gctcggcgtt
      421 tttgagcgcg tcgtcaagcg tctggccgga atgcggggag gtctggacca gccgccagcc
      481 gtcatcatag tcgcggaaac ccactgttga tttgtagcgg agctcgccgg agtacagggc
      541 tgcgccgact tcattcaggg gggcccactt ccagatgaat tcgacatcag cgagattgcc
      601 ctgcttgctg attccggtga tctcgagcaa ttcgcgccgt gcgacaggaa tggcgaagga
      661 aggcttgctg gcttcttcgg cagaaaccag cgcgcgaatc gtctccacgc ccgagggagt
      721 gagcagaact ccccaacacg gaggcggtgc caggccctgc gagcaggggg agttcgtagc
      781 ggaaatccag ccgtgatgtt gcagcaccag gtattcggga gaggcgtagg cgctgttgga
      841 aaggatcccg gtttgcaata cgaattgctg gcgaactttg aaggcatcgg aagcggagag
      901 caggtcgaaa gccaggcggc gagtgaggaa gtcgcgcggg gagcaggagg cgagcaacag
      961 acacaacaga caaagaatgg gtttccttcg catgagtcag actcacccaa aaccggactt
     1021 cgtccggcgg acagccgagg gcggctgtcc ccacacgatc cgtggatcaa cgaccagtga
     1081 ttgtaaacaa acgggagcgc gaacgcggca agaaaggatg ggaggcgatc tggccgtcaa
     1141 actttgccgc ccagcggccg ggaatctgcg cggcgcaagc ggagcaccgg ccttcgtcgg
     1201 tcaggcggta gtcctcgatc aaatagccgt ggcgactgat cagagtctca tcgcattgcg
     1261 ggcagcgggt atcttcccac tgccccaccc ttccgggaag atttccggca taaatgtagc
     1321 gcaggccggc ctgttgcccg atttcggcgg cgcgcaacag atcatcgacg ctggtgtcgc
     1381 gctggtcggt cattttgtag tcggcgtgaa acgcggtcac gtgccaagga atgtcaggag
     1441 aaatgctcgc gagaaattcg gccatgcggc gcagttcgtc ggcggagtcg ttgaacccgg
     1501 ggatgagcag agtgacaatt tcgagccaca agcccatctg atgaatccgc cggatgctgt
     1561 cgaggatcgg gccgatgcga ccgccgagtt cgtggtagcg gcgatcgtcg aaactcttga
     1621 ggtcaacctt gtaaagatcg agccaggggc gcaagtaatc cagaacctgt ggcgtggcat
     1681 tgccgttgga gacgaatccg gtgagaagtc ctgcagcttt cgcctccttg aaaatggcga
     1741 cagcccattc gctggtgatc aaaggctcat tgtaggtgct gaccacgacc cgcgcgccct
     1801 gctcgacggc ctcccgcacc agatgttcag gcgaaacttc gagcggcggc gatacggctt
     1861 ccggatcgcg caatgcctgc gacgtaaccc agttctggca gtacgcgcag tgcaggtcgc
     1921 agcccatcat gccgaagctg tatgcgaggg cgcccggatg cacgtgaaag aagggtttct
     1981 tctcgatagg atcacactgc attcccgcgg tgtatcccca ggggacgtag agcgtcccgc
     2041 cgcggttata gcggactttg cagactccgg gctggccttg aggaatggga cagcaatgac
     2101 cgcaggagaa gcagcggacg cgctggtttt cgagggtctg atacaaggat gcgtcagctt
     2161 cgcggactct ttgatcgaga atgtcccgaa gcgtgggcat tgc
//
LOCUS       DASPUL010000439         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_352515, whole genome shotgun sequence.
ACCESSION   DASPUL010000439 DASPUL010000000
VERSION     DASPUL010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352515"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..683
                     /locus_tag="VEJ00_10495"
     CDS             186..683
                     /locus_tag="VEJ00_10495"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXX01634.1"
                     /translation="MTALATGKKAPQFELKTLDGKPFSLNEALTDGAVVLAFFKVSCP
                     VCQYAFPFLERLHKAYRQKGHRLVGVSQNDAKETAAFNKEFGVTFPVLLDDTRGYPVS
                     NAYGLTNVPTLFWIAEDGGIEVSSVGWVKADIEEISRRMAESGRIAPAAIFKPGENVR
                     DFQAG"
     gene            1071..2771
                     /locus_tag="VEJ00_10500"
     CDS             1071..2771
                     /locus_tag="VEJ00_10500"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXX01635.1"
                     /translation="MPRTFSVSLMVCLSLLCLSRTAWPDGGAGAAKGAKTPSVRLPGE
                     AYKALETISPERIRWHVRYLSHDLLEGRGTGQRGGDIAAEYIATQFAEYGLKPAGDHG
                     TYMQKVPLVGITTLPETTFSLIPKQGETMNLKPLDEYVATDQTQQAQSEVDADIVYVG
                     YGIEAPEYNWDDYKGTDVRGKVLLMLVNEPPSDDPKFFKGKALTYYGRWTYKYEEAAR
                     KGAVGVLLIHQTQMASYPWEVVRNSNSGEKSYLKLEGPALKVASWIHLDVAKRLASAS
                     KMDLDKMMQDAQSRDFHPVNLGARLKAHMVSKVRNFESNNVVAILPGSDRKVADEAVI
                     YTAHYDHFGIRPDMPGDNIFNGAQDNATGCGILLELARAFGAAAQKPRRSILFAAVTA
                     EEQGLLGSEYLGKHPPIPAGKITLDLNYDDVKPLGAPEEVEVSGAERTTFYPEVQAMA
                     KDFRLAIRPDARPEAGHFYRSDHFSLARVGVPSFSINEGMKYKGHTEAWGMEQENEFV
                     TKHYHQPSDEYHPEMDFTGDSAMARFGFALGWEAASLPKLIGWQKADEFEAARVKSQQ
                     "
     gene            complement(2982..>3240)
                     /locus_tag="VEJ00_10505"
     CDS             complement(2982..>3240)
                     /locus_tag="VEJ00_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01636.1"
                     /translation="SPPGKPCLTLCRSRSDVGRAMITGKCADVGKVKGPTLRGLAAHA
                     PYFHNGAAARLDDVVEFYKQRFNLNLTAQQKSDLVAFLQTL"
BASE COUNT          675 a          902 c         1055 g          608 t
ORIGIN      
        1 gggcttacaa ggaaaatcct gcggacgccg ggattgttga agggcgtcgg tagtgcatca
       61 tgacgcaatc ttcgacgttg gcatctcgtt gaatctggtg actccggccg catcggttgc
      121 cacagtgagc ccgcctcgcc ttctcaacga tcgaataaaa tagagtcact gaagaggaga
      181 gaccgatgac agcgctggcg accggaaaga aagctcccca gttcgaactg aaaactctgg
      241 acggcaagcc cttttctctc aacgaggcgc ttaccgacgg ggctgttgtg ctggcctttt
      301 tcaaggtttc gtgtcctgtc tgccagtatg cgttcccgtt tctggagcgg ctgcacaagg
      361 cgtaccggca gaaggggcac cggctggtcg gggtgtcgca gaacgacgcc aaggagacgg
      421 cggcgttcaa caaggaattt ggcgtaacgt ttccggtgct gcttgacgat acgcgcggct
      481 acccggtgtc gaacgcttac gggctgacga acgtgcccac tcttttctgg atcgccgagg
      541 atggcgggat cgaagtttcg agcgtgggat gggtaaaagc cgatatcgag gagatcagcc
      601 gccggatggc ggagtcggga aggatcgctc cggctgccat cttcaagccg ggcgagaatg
      661 tgcgcgattt ccaggctggt tgaagctcga aaaactagcg ccgggtccgg cgcttccggg
      721 aaaaagcacc aaccacagag ggcacagggc aacgctgggt accgcctgcc ggacagggcc
      781 ttgtggagcg actgttcctt ggtcggtgca ctctcgtgcc ctgtctggcc gactcgtttt
      841 gccagatcag ggcgggtctt tcctttacaa ttcttcaaga ctttcacaaa gaccatttca
      901 taggtgttgc cgggctggca gccgcgaacc taaggggctg aagccctcat tcttcgcgcg
      961 ccttgaccgg cacgactaaa gtcgtgccct tcccggccaa ctatgatacg gatctcaagc
     1021 cttccagccg gggactgcct ggccagggga gaaacctacg aatgcgacga atgccgagaa
     1081 ctttttcggt cagtctgatg gtgtgcctca gcctgctatg ccttagccgg acagcgtggc
     1141 ccgatggagg agcaggagcc gccaaggggg ccaagactcc gtcggtgcgg ctgccgggcg
     1201 aggcctacaa ggcgctggag acgatctctc cggagcgcat tcgctggcac gtgcgctatc
     1261 tgtcgcacga tctgctggag ggccgtggca ctggacagcg cggcggcgat attgccgcgg
     1321 aatacatcgc gacccaattt gccgaatacg ggctgaagcc ggcgggagat cacggaactt
     1381 acatgcagaa agtgccgctg gtcgggatca cgacgctgcc cgagaccacg ttctcgctga
     1441 tcccgaagca gggcgagacc atgaatctga agccgctcga cgagtacgtg gcgacagatc
     1501 agacccagca ggcgcagtcg gaggttgacg ccgacatcgt ctacgtgggt tacgggatcg
     1561 aggctccgga atataactgg gacgactaca aaggcaccga cgttcgcggc aaagtgctgc
     1621 tcatgctggt gaatgagccg ccgtcggatg acccgaagtt tttcaaaggc aaggccctta
     1681 cctactacgg gcgctggacc tacaagtacg aagaggcggc gcgcaagggc gcggtgggcg
     1741 tgctgctgat tcaccagact cagatggcga gctatccgtg ggaggtagtg cggaactcga
     1801 attcgggcga gaagtcctat ctgaaactgg aaggtccggc gctgaaggtg gcctcgtgga
     1861 ttcacctgga tgtggcgaag cggctggcct cggcgtcgaa gatggatctc gacaagatga
     1921 tgcaggacgc gcaatcgcga gactttcatc ccgtgaatct gggtgcgcgg ctcaaggcgc
     1981 acatggtcag caaagtccgc aattttgagt cgaacaacgt ggtcgcgatc ctgccgggct
     2041 cggatcgcaa ggtggccgat gaagcggtga tctacacggc acattacgat catttcggca
     2101 ttcgtccgga catgccgggg gacaatatct tcaatggagc acaggacaac gcaaccggct
     2161 gcggcattct gctcgaactg gcgagggcgt tcggggcggc cgcgcagaag ccgcggcggt
     2221 cgattctttt tgcggcagtg accgccgaag agcagggcct gctcggctcg gaatacctgg
     2281 gaaagcatcc gccgattccg gcgggaaaga tcacgctcga tctaaactat gacgacgtca
     2341 agccgctcgg agcgccggag gaagtcgaag tttccggggc ggagcggacg acgttttatc
     2401 ccgaggttca ggccatggcc aaggatttcc ggctggcgat tcgtccggat gcgcggccgg
     2461 aggcagggca cttttatcgc tccgatcact tcagcctggc gcgcgtaggc gtgccgtcgt
     2521 tttcgatcaa tgaggggatg aagtacaagg ggcacaccga agcctggggg atggagcagg
     2581 aaaatgagtt tgtaacgaag cattatcacc agccaagcga tgagtaccat cccgagatgg
     2641 atttcaccgg agactcggcc atggcgcgat tcggattcgc gctgggatgg gaagcggcga
     2701 gtttgccgaa gctcatcgga tggcagaagg ccgacgagtt cgaggcggcg cgagtaaaga
     2761 gccagcagta gctactagtc gcgagtaaag cgtgtccacc ggcgagcagc taacgacatt
     2821 gacgcggccc tgaagggccg ctcttccacg gaccttgcgg cgcaccgttt ctacgaattt
     2881 gcgcttgctc tgcgaggtgc tgacacgccg ctccccgcaa cggcagttca tgcccacaga
     2941 tcctcacgct gcatcccttt tacggccacg cggcggcgcc atcacagagt ctgcaaaaag
     3001 gcgacgagat ccgacttctg ctgcgccgtc aggttcaggt tgaagcgctg cttgtagaac
     3061 tcgacgacat cgtcaagccg ggcggccgcg ccgttgtgaa agtagggcgc gtgtgcggcc
     3121 aaaccgcgca gggtcggacc cttcacctta ccgacatcgg cgcacttgcc cgtgatcatg
     3181 gcgcgtccga catcggatcg ggatcggcaa agtgttaaac agggcttgcc cgggggcgac
//
LOCUS       DASPUL010000440         7068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_224357, whole genome shotgun sequence.
ACCESSION   DASPUL010000440 DASPUL010000000
VERSION     DASPUL010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224357"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..505
                     /locus_tag="VEJ00_10510"
     CDS             329..505
                     /locus_tag="VEJ00_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01637.1"
                     /translation="MDFQINDETYFADLGDGEWQVFVSTPNGARPIPVYDDGGDFGDA
                     MALVEDKRTRKIVN"
     gene            544..1779
                     /locus_tag="VEJ00_10515"
     CDS             544..1779
                     /locus_tag="VEJ00_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HXX01638.1"
                     /translation="MATTTKKDYYEILGVKKSASAEDIRKAFKKLARKYHPDVNPGDK
                     AAEEKFKTLSEANEVLSDPKKRKIYDQVGFYSDNIDPATAEAYARAGGGTASGGFPGG
                     FPGGAQTGGQGVPFDFGGFDFSDLMDNAGRARKTSGGGGGSFRDIFGSIFGGGRAAPA
                     EEGPEPGTDLEYQVNVPFWTAIRGGVMRLNVTRQDVCSTCHGQGELESPGKCPECGGT
                     GQITQTGGRMKFNVQCPRCHGTGKNLTTCPTCHGEGTVTKTEPLEVRIKAGTRDGQRI
                     RVPGKGNAGAHGGAPGDLYAIIRTGEHPIFRREGDDIYLTVPVNATEAALGAKVEVPT
                     IDGRALLKIPPGTQSGQKLRLREKGVPSATREGERGDEIVEITVHVPMPRDERTKELL
                     RELAKLNPEDPRAELWSKV"
     gene            complement(1795..2313)
                     /locus_tag="VEJ00_10520"
     CDS             complement(1795..2313)
                     /locus_tag="VEJ00_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01639.1"
                     /translation="MSGMIESEQLAAHYGLQTDEELLRLKEESEQLTPAAREQLNNEL
                     QRRRIGAAAKQALRVSDEIDTGDDTAAKFSILFPSLGRVAATLKDWRHYRSQTGAWPV
                     FSIAAHVIHGVFLVGWAGYLVWFGVTHKWSTTKLVLVSLPLLLIDTILENWIEGKIRT
                     GELQRYRTKRSK"
     gene            2674..4005
                     /locus_tag="VEJ00_10525"
     CDS             2674..4005
                     /locus_tag="VEJ00_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXX01640.1"
                     /translation="MRLTLWPLVAATFFMVSGGTYGTEDIVHGAGYGRAILILLITPL
                     LWSLPTAFMIGELSSALPHEGGYYAWVRRAMGNFWGFQEAWLSLVASIFDMAIYPTLF
                     VTYLTRMFPWFQESHRGLYVGMAVVIACAVLNICGVKVVSLTSLWLFLALSAPFVAIV
                     LLAPFKYGALLHAVTKPTTNGVDILGGLLVCMWNYMGWDNASTIAAEVERPQRTYPRA
                     MLVAVCIVALSYVLPFAAMWMTGLTPQAWETGSWADIAGLLGGPLLRIAVVLGGIISA
                     FGMFNALVMSYSRLPLAMAQDGLLPKVFGKLHPKSKAPWIAIVALACFWMPCLLLGFE
                     RLVTLDILLYGGSLLLEFIALAVLRFTEPDLPRPFRVPGGLFGAIAIGIAPMLLLGFS
                     VFRSEHESILGMSSFAFGMILIAAGFAAYYVNHALKPDGWSVPPEQAQPAA"
     gene            4091..4492
                     /locus_tag="VEJ00_10530"
     CDS             4091..4492
                     /locus_tag="VEJ00_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXX01641.1"
                     /translation="MTKRKSKGAYMISAVAEMYGIHPQTLRLYEREGLLKPSRTEGNT
                     RLYTDEDLQRLEFILNLARDLGVNIAGMAIILQMRERMEEMQRQIQEFVQYIQQEVLT
                     RASAATDPSKGAIVPVRRPLVAPAVAVGRKK"
     gene            4564..5565
                     /locus_tag="VEJ00_10535"
     CDS             4564..5565
                     /locus_tag="VEJ00_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HXX01642.1"
                     /translation="MLPFKLVYSDAYYLPIGEHVFPAEKYRRVRDRLIASRVAEAADF
                     LEPQPATDQDILLVHKPEYVQKLKTGTLSAREEMEMEVPYSRDLVEAFWLSAGGSILA
                     ARRSLADRVSISIGGGFHHAFPDHGEGFCMIHDVAVAIRRLQRDDKIRTAMTVDCDVH
                     QGNGTASIFAGMRTASSLLPSVSSSTLEGPHGKPRGGKMREAHAGDVFTISLHQHNNY
                     PLWKPPSSIDVDLPDGIGDDDYLAWLDNALSSGLRQFEPDLICYIAGADPYIEDQLGG
                     LSLTIAGLKKRDELVFKVARARSIPVMVTFAGGYAQNIEDTVTIHSNTVGAAKEIFS"
     gene            complement(5568..6287)
                     /locus_tag="VEJ00_10540"
     CDS             complement(5568..6287)
                     /locus_tag="VEJ00_10540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXX01643.1"
                     /translation="MASTELAQESSHPAVAPAWHTVVVLVVLLGLSFAGAINKNLSPI
                     GRPHGRAVGYIVVMVFEWIMVAFIWFGVSRRGVRVSDLVGGSWARPITILRDLGIAVG
                     FLIVSGLILNGIGHLLKVAPNEAIRNMFPRGRAEAIVFLMLAMTAGFCEELIFRGYLQ
                     RQFAALTRTAAGGIILQGIAFGAGHGYQGWRYMVVIAVFGTLFGLLAWWLRTLRPGMI
                     AHFVQDGAGGLLGPHMLKHIL"
     gene            complement(6356..>7068)
                     /locus_tag="VEJ00_10545"
     CDS             complement(6356..>7068)
                     /locus_tag="VEJ00_10545"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX01644.1"
                     /translation="KALGIAWAFFLDAISFLFIIAALWRLPDPPAPAVAASKRAVLPL
                     ILEGLTYVRKDVPLRSLLLLAAVINFCIAGPVGVGLAYLAKTRFGSPAAYGIVVSAAA
                     AGGLLGSLLAGVWRVRKRGMLMISVPVVIGVCLGMLGWLTHLWMVAAVLLLMGASAGV
                     TNVHIASWIQQRIEPAVRGRVMSVLMLSVFGLMPVSLAMAGVLVAWSLRWMFVLASVL
                     LLVIAAVAAVQKPVRQIA"
BASE COUNT         1442 a         2149 c         2194 g         1283 t
ORIGIN      
        1 cctgtcatcc tgagcggagc aggcggttcg cgaagcggac cgcctgcgga gtcgaaggaa
       61 ccgcgtgccc gcggacagtg agatgaaccg cgcaaggcgt tctaacgatc aggacggctg
      121 acgagagaga acccctcgag gaactgttac cgctgcaggc tggcatgggg tccttcgctt
      181 cgctcaggat gacagggttt tgaggtcgct gtgcgaaaga tgcgtgcacg gtgtccttcg
      241 actgcggttg atcttcgcgc tgtggcgcga agatcaatcc tcgctcagga tgacagattt
      301 ttagttcaca ctctcagggg agatcgaatt ggactttcag atcaacgacg aaacgtactt
      361 tgcggacctc ggcgatgggg agtggcaggt gtttgtgtca acgccgaacg gggcacggcc
      421 gatcccggtc tatgacgacg gaggcgactt cggcgacgcg atggcactag tagaagacaa
      481 gcggacacgt aagatcgtaa attaggcgag gttcggcgcg gttcacaggt cccaggctaa
      541 ggaatggcaa ccacgaccaa gaaggactac tacgaaatcc tcggggtgaa gaagtcggcc
      601 tccgccgaag atatccgcaa ggctttcaag aagctcgccc gcaagtacca tcccgacgtc
      661 aatcccggag acaaggctgc ggaagaaaag ttcaagacgc tctccgaggc caacgaagtt
      721 ctcagcgacc ccaaaaaacg aaaaatctac gaccaggtcg gattctactc cgacaatatt
      781 gatccggcga cggccgaggc gtatgcgcgc gcgggaggcg gcactgcgag tggaggcttt
      841 ccggggggat ttcccggcgg ggctcagacg ggtggacaag gtgtcccctt cgacttcggg
      901 ggtttcgact tttccgacct gatggacaac gccggccggg cacggaaaac gagcggcggg
      961 ggcggaggca gcttccgcga catcttcggc tcgatctttg gtggtggacg ggctgcgccg
     1021 gcggaggagg gtcctgagcc gggaacggac ctcgaatatc aggtcaatgt tcccttctgg
     1081 acggcgatcc gcggcggggt catgcggctc aacgtcacgc ggcaggacgt ctgttcaacc
     1141 tgccacgggc agggggagct ggagtctccg ggaaaatgtc cggaatgcgg cgggacgggg
     1201 cagatcaccc agactggcgg ccggatgaag ttcaacgtgc agtgtccgcg gtgccacggg
     1261 acgggcaaga acctgacgac gtgtccgacc tgccacggcg agggcacggt caccaagacc
     1321 gagccgctgg aagtgcgcat caaggccggg acgcgcgacg ggcagcgcat ccgggtgccg
     1381 ggcaagggca acgccggggc gcatggtgga gcgccgggcg acctgtatgc gattatccgc
     1441 accggggagc atccgatttt ccggcgcgag ggggacgaca tctacctgac ggtgccggtc
     1501 aacgcgaccg aggcggcact gggggcgaaa gttgaggtgc caaccatcga cgggcgggcg
     1561 ctgctgaaga ttcctcccgg gacgcaatcg gggcaaaagc tgcggctgcg cgagaaagga
     1621 gtgccttcag ccacgcgcga aggcgagcgg ggcgacgaga ttgtcgagat caccgtccac
     1681 gtcccaatgc cccgcgacga acggacgaaa gagctgctgc gggagcttgc caagctgaac
     1741 ccggaagacc caagagcgga gttgtggagc aaggtgtagc cggctcttcg tggcctactt
     1801 gctgcgtttt gttcggtagc gctgtagctc tcccgtccga atctttcctt caatccagtt
     1861 ttcgagaatc gtgtcgatca gcaggagcgg aagcgaaaca aggacgagct tcgtggttga
     1921 ccacttgtgg gtgacgccga accagaccag atatcccgcc cacccgacca aaaaaactcc
     1981 atgaatcaca tgggcagcga tggagaagac cggccacgct ccggtctggc ttctgtagtg
     2041 cctccagtcc ttcagcgtcg cagcaactcg gcctaggctg gggaatagga tggagaactt
     2101 cgcggcggtg tcgtcccccg tatcgatttc gtcgcttacc cgcaaagcct gtttcgcggc
     2161 tgcgccgatt cttctccttt ggagctcgtt gttgagctgc tcccgggccg ccggagtcag
     2221 ctgctctgat tcctccttca gtcgcaggag ttcctcatcg gtctgaagcc cgtaatgcgc
     2281 ggcaagctgc tccgactcga tcatccccga catgctacca ctctctgcac acccacaccc
     2341 tcaacctcca agtgcggacc tcagcgaccc tcgttacctc cgtaccagcc cggctgctta
     2401 caaaattttg caaaataatc gaaaaaaaac ttgcatctgt ggaaatttgc cgtaatctca
     2461 caagtgcgaa ctaacttcgc cgaaatgccc gggaaatcgc tggattccgg gagccgcgag
     2521 agaacagtca agggagacgc ggtggtgaga gtgagtcgct tgaggaggaa gccatagaaa
     2581 ttggcaacag ctagccagcc acatccacaa cccgacaacc cccaaaccct cgccgccgta
     2641 gcaaaccgga atcagctagc ccccaggaag tttgtgcgcc tcacgctttg gcccctggtc
     2701 gccgccacct tctttatggt gtcgggcgga acctatggca ccgaagatat cgtgcatggc
     2761 gcgggctacg gacgcgcgat cctgatcctg ctcatcacac cgctgctgtg gagcctgccg
     2821 acggcattca tgatcggcga gctttcgagc gcgttgcccc acgaaggcgg ctactacgcc
     2881 tgggtgcgcc gcgctatggg caatttctgg ggatttcagg aagcctggct ctcgctggtc
     2941 gcgtccattt tcgacatggc gatctatccc acgctgttcg tcacctacct cacgcgcatg
     3001 ttcccgtggt ttcaggagag tcatcgcgga ctgtacgtgg gcatggcggt ggtcatcgcg
     3061 tgtgcggtgc tgaatatctg cggcgtgaaa gtcgtctccc tgacttcgct gtggctgttc
     3121 cttgcgctct cggcgccctt tgtggcgatt gtgctgctgg ccccgttcaa atacggcgcg
     3181 cttctgcatg ccgtcaccaa gccaactacc aacggcgtcg acattctcgg cggactgctg
     3241 gtttgcatgt ggaattacat gggctgggac aacgcctcga caatcgccgc ggaagtcgag
     3301 cgcccgcagc ggacgtatcc gcgggcgatg ctggtcgcgg tctgcattgt ggctctgagc
     3361 tacgtactgc cgttcgccgc gatgtggatg accggcctta cgccgcaggc gtgggagacc
     3421 ggttcgtggg ccgacatcgc cgggctgctg ggcggcccgc tgctgcgcat tgccgtcgtg
     3481 ctcggcggca tcatcagcgc cttcggcatg ttcaacgcgc tcgtaatgtc gtattcgcgc
     3541 ctgccgctgg ctatggcgca ggacggcctg ctgccaaagg tcttcggcaa gctgcatccg
     3601 aagtcgaagg cgccgtggat cgcgatcgtg gccctggcat gcttttggat gccgtgcctg
     3661 ctgctcggct tcgagcgcct ggtgacgctc gacattcttc tctacggcgg gagcctgctg
     3721 ctcgagttca tcgctttggc cgtgctgcgg tttaccgagc cggacctgcc gcgcccgttc
     3781 cgcgtgcccg gcggcctgtt tggggccatc gcgatcggca tcgcgcccat gctgctgctc
     3841 ggattctccg tattccgcag cgagcacgaa tcgattctgg gcatgagttc gttcgccttc
     3901 gggatgatcc tgatcgccgc cgggttcgcc gcctactacg tcaaccacgc gctgaagccg
     3961 gacggatggt ccgtcccgcc ggaacaagcg cagcccgcgg cgtaactttg tcgccacgga
     4021 tttacgcgga ttttttctgt gaagatccgc gacaatccgt ggccattttg tttctgctat
     4081 aaatagtcgc gtgaccaagc gcaaatccaa aggtgcgtac atgatctcag ccgtggctga
     4141 gatgtatggc atccatccgc agaccctgcg gctgtacgag cgggaagggc tgctcaagcc
     4201 ctcgcggaca gaaggcaaca cgcggctcta taccgacgaa gacctgcagc ggctcgagtt
     4261 catcctgaat ttggcgcgtg acctgggagt gaacatcgcg ggcatggcca tcatcctgca
     4321 aatgcgcgag cggatggagg agatgcagag gcaaatccag gagttcgtgc agtacatcca
     4381 gcaggaagtg ctgacccgtg ccagcgctgc aaccgaccct tcgaaggggg ccatcgtgcc
     4441 ggtaagacgg ccgcttgtag caccggccgt tgcggtcggg cggaaaaagt agggctcccc
     4501 acaagctttc gcgcgtatta tatggggttt ctcggcaccg actggccgag cgctaaacgc
     4561 ggaatgctgc ccttcaagct cgtttacagc gacgcttact acctgcccat cggcgaacac
     4621 gtcttccctg ccgaaaagta ccgccgcgtt cgcgaccggc tgatcgcgag ccgggtggcc
     4681 gaagccgctg actttctcga gccgcagccc gccaccgatc aggacattct cctggtgcac
     4741 aagcctgaat acgtgcagaa gctgaagacg ggcacgctca gtgcgcgcga agaaatggag
     4801 atggaagtcc cctattcccg tgatctggtg gaagcgtttt ggctgtcagc gggcggctcg
     4861 attctggcgg cgcggcgaag cctggccgac cgggtttcga tcagcatcgg cggcggattt
     4921 catcatgcct ttcctgatca tggcgaagga ttctgcatga ttcacgacgt ggcagtggcc
     4981 atccggcggc tgcaacgcga cgacaagatt cgcactgcca tgactgtcga ctgcgacgtg
     5041 catcagggca acggtacggc ttcgatcttc gccggcatga ggacggcgag ttcgctgctg
     5101 ccttccgttt cgagttcgac gctggaaggc ccccacggca agccgcgcgg gggaaagatg
     5161 cgcgaggccc atgccggcga cgtgttcacg atttcactgc atcagcacaa caactatccg
     5221 ctgtggaagc cgccatcgtc gatcgacgtg gacctgcccg acgggatcgg cgacgacgac
     5281 tacctggcct ggctggacaa cgcgctcagc tcgggcctgc ggcagttcga gcccgacctg
     5341 atttgctaca tcgccggggc cgatccatac atagaagacc agctaggcgg cctttcgctc
     5401 acgatcgcgg gattgaagaa gcgggacgag ctcgtgttca aagttgctcg cgcccgaagc
     5461 atcccggtga tggtgacgtt cgccggcggg tacgcccaga acatcgaaga cacggtgacg
     5521 atccacagca ataccgtggg cgcggccaaa gagatatttt cctgacgcta cagaatgtgc
     5581 ttcagcatgt gagggccgag aaggcctcca gcaccatcct gcacgaagtg cgcgatcatg
     5641 ccggggcgca gagtccgtag ccaccaggcc agcaggccga acagggtgcc gaatacggcg
     5701 attaccacca tgtatctcca gccttgatat ccgtggccgg cgccaaaggc aatgccctga
     5761 aggatgatcc cgccggcggc ggtccgagtc aatgcagcga actggcgctg cagatatccg
     5821 cggaaaatca gctcttcgca gaagccggcg gtcatggcca gcatcaggaa aacaatcgct
     5881 tccgcacgtc cgcggggaaa catattgcgg atcgcttcat tcggagcaac cttgagcagg
     5941 tgcccgatcc cgttcaggat gagcccggag acgatgagga atccaaccgc gattccgaga
     6001 tcgcgaagga tagtgatcgg gcgcgcccaa ctgcctccga caagatccga cacacggaca
     6061 ccccgccggc ttacgccgaa ccagatgaac gccaccatga tccattcgaa gaccatgacg
     6121 acgatatacc cgacggcacg cccgtgaggc cgaccaatgg gcgacaggtt tttgttgatc
     6181 gcgccggcga acgagagccc gagcagaact acgaggacca cgacggtgtg ccatgcgggt
     6241 gcgacggccg ggtgtgagga ctcctgtgcc agttctgttg acgccatgtt gatttgccct
     6301 ccgaagtgct aatacgttga gccctaaata aacgttcccc ggcttgcgga aacgctcacg
     6361 cgatctgccg taccggtttc tgcacggcag caaccgccgc gatgacgagc aaaagcacac
     6421 tagcaaggac gaacatccag cgcaaactcc aggctaccag caccccagcc attgccagag
     6481 agaccggcat gagtccgaat accgagagca tcagaacact catcacccgg ccgcgcaccg
     6541 ccggctcgat acgctgctga atccacgagg cgatgtgaac gttggttact cctgcgctcg
     6601 cgcccatcag caggagcacg gcggccacca tccacaaatg ggtcagccat cccagcattc
     6661 ccaggcatac gccgatcaca accggcaccg agatcatcag cattccgcgt ttgcgcaccc
     6721 gccaaactcc agcgagcagg gagccgagca gtccgcctgc cgcggcagcc gacaccacta
     6781 tgccgtatgc cgcaggcgag ccgaatcgcg ttttcgccag gtacgccagc ccgaccccga
     6841 cgggtccggc aatgcagaag ttgatgacag ccgccagcag caacagtgag cgcagcggca
     6901 cgtctttgcg gacatacgtc aagccttcca gaattaaagg cagaactgct cgcttgctcg
     6961 ccgcgaccgc aggcgcggga ggatcgggca gccgccacaa ggcggcaatg atgaacagaa
     7021 agctgatggc gtcgagaaag aacgcccacg cgatgcccag cgccttga
//
LOCUS       DASPUL010000441         8571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_491750, whole genome shotgun sequence.
ACCESSION   DASPUL010000441 DASPUL010000000
VERSION     DASPUL010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8571)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8571
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491750"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2046)
                     /locus_tag="VEJ00_10550"
     CDS             complement(<1..2046)
                     /locus_tag="VEJ00_10550"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXX01645.1"
                     /translation="MTDPSGAAISGATAVMTPATGSPLTATTNSQGMYEFKDLAAGKY
                     ALTVVAQGFTLYENDSVMIADQPLRLNISMTIQVETQKVQVSDTAPTIDVNPASNAGA
                     IVISGKELEALPDDPDELLQDLQALAGPSAGPSGGQMYIDGFTAGQLPPKSSIREIRI
                     NQNPFSSEFDKLGYGRIEIFTKPGTDKFHGEFQINGNDSAFNSSNPFGGAEPGYDSEQ
                     YSGSVSGPVNKNASFFFSFQRRNINDLSAITADTLDATTFALVPIAESVPFPHQRTNI
                     GPRLDWALTKNNTLTARYQYWRDTETNDGLGQFTLPSQGYNSKNIEHTIQIGDTQIYG
                     AKVVNETRFQYLRELNFQTPADTNPSVGVPGAFSGGGSGTLSNDHQDHYELQNYTSVI
                     HGNQTIKFGGRLRANREANFSTSGFNGSFTFSLLQTDPNTGAGTPADCQNFTAGEHPN
                     PPCPISLQYALQNPASGLATQLRYTTGLPTADVLTFDAGLYIQDDWRVRPNITLSGGL
                     RFESQNYIQDHADWAPRVGIAWGVGGRSGPPKVVIRGGAGIFYDRFALSNILQAQRLN
                     GTTQRQFTVNNPTCFTQVDQPLANFSNCGATDSSSVTYKTSPRFRAPGTLQAAVSVER
                     QLTKTSTLSLTYLHSRGFDQLATINANAPFPGTPCFPSCAIPPNGALYEYVSEGVFKQ
                     "
     gene            complement(2218..3477)
                     /locus_tag="VEJ00_10555"
     CDS             complement(2218..3477)
                     /locus_tag="VEJ00_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01646.1"
                     /translation="MYIKLWGGMSTKRKFIVPFILLTSAIAAHTAELLLAPALLAAQT
                     PAQSASGQSAATRRIGAIKAINGSTIALAPDSGSEITVTVQPGTRVLRLAPGEKDLKN
                     ATPIPFQDLQVGDRILVGGAASEDTKSIAASVIVAMKRSDLEARQKEDLQDWQKRGVD
                     GVVKALDPASGTISLSVPGFGGGKEIAVHTTKATVMLRYPAGSANFADAKPSSLSEMR
                     TGDQFHARGDRSADGSELTAEEVVFGTFRNIAGTINSVDATSGTIDVQDVLSKKSLQV
                     KVNAGSQLRKLPAEAARLVLGFKGGAADRPASGSNPAPVSAGGEGPAAPGGMSPGGSG
                     SAGGPRLGRFDLNRVLSRMPAVGLSDLHKGDAVMIVAAEGRDGDVITKLLAGVEPILQ
                     AAPSAGQALMLTPWNLGGAPSGDNGNP"
     gene            3699..4337
                     /locus_tag="VEJ00_10560"
     CDS             3699..4337
                     /locus_tag="VEJ00_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01647.1"
                     /translation="MLVPISVLGQTPSAILHAQGGVWVNGYEGKDSTAIFAGDLLETK
                     PEFSAKLTLEGSEALIQAESVVKFQGDLLALDHGGVAVGTSRGLKVKVNCITVVPVRN
                     EWTQYEVVDVNGTVQVAARKGDVNVEHEMGKGKPELGAEGSRGGTVHEGEQKSYSESE
                     LCGAPPGPTGGSSGLNPKYIGIGGAVGGGILLCVILCRGGGSTKTQISPDKP"
     gene            complement(4349..5557)
                     /gene="ilvA"
                     /locus_tag="VEJ00_10565"
     CDS             complement(4349..5557)
                     /gene="ilvA"
                     /locus_tag="VEJ00_10565"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005600.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HXX01648.1"
                     /translation="MVNLSDVRAALDRIRGDIRVSPCTHSETFSNLTGNSIFLKLDNQ
                     QRTGAFKERGALNKLLTLSQEERKRGVIAASAGNHAQGVAYHAGRHSIRARIFMPLPT
                     PLTKVSSTRAYGAEVILEGANYDEAYEKAVENGARDHLTLIHAFDDEAVIAGQGTLGL
                     EILEQQPDIEAIVVPIGGGGLIGGIACAAKEMNPAVKVYGVQPARIPSMKAAIAAGKP
                     VALRSATTIADGIAVRRAGERTFPLVQKYVDEIATVEEEEIANAILLLLEREKTLAEG
                     AGAAALAAVLNRKLPLAGQRVAVLVCGGNIDVTLLARIIERGLVKDGRLVRLRVHLPD
                     YPGALHRLTGILADHRANIVETAYDRAYHGVNLGDTAIDITMETRGPDHIAELLSALL
                     SAGYTHERIL"
     gene            5734..6636
                     /locus_tag="VEJ00_10570"
     CDS             5734..6636
                     /locus_tag="VEJ00_10570"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXX01649.1"
                     /translation="MDFDQLITFLEVAKQGSFSRAGEKVFRSQSAVSAQIRQLEQEYG
                     DRLLDRSGKTVKLTPAGQVFFEYAQRLRSLRDESLTAVADHSGTPRGTLRVGANEATC
                     LYVLPEVFGEYCRRYPPVQISIYRNFSYKIVEKLENGAVDVGILTLPIQSPSLKIQPI
                     FRDKLVVMVSPKNPLAKHKVVPVSEIVKYPLLFPKTGHTRRMLDKLFRPYQAQVQVRM
                     ELPSIGMIKSFVAADLGVSLISASFARDEVDAGRVKLIDLEDAELYRELALAYRRDRT
                     LSVAATAFVSMLRHMAIPSKKTDA"
     gene            complement(6653..7450)
                     /locus_tag="VEJ00_10575"
     CDS             complement(6653..7450)
                     /locus_tag="VEJ00_10575"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HXX01650.1"
                     /translation="MKDQITSGFESQSQLQAPLTPQLAGQLAVAEVQSAAETWSPHRV
                     LAWAFETFGNRIAISSAFGAEGMVMIDIASRVHKNFRLFTIDTEFLFPETYNLMDRIE
                     EKYGITIEKVMSVLSPEEQERIHGEALWSRDPDLCCNLRKVEPLRLKLKTLDAWVTSI
                     RRDQTSHRAGAHRVEWDAKFGLVKVNPIVDWNSKQVWRYIHDHDVPYNQLHDMDYPSI
                     GCTHCTRAVRPGEDPRAGRWPGFAKKECGLHMIEPAPEATSRRAEGD"
     gene            complement(7447..8277)
                     /gene="cobA"
                     /locus_tag="VEJ00_10580"
     CDS             complement(7447..8277)
                     /gene="cobA"
                     /locus_tag="VEJ00_10580"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR01469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HXX01651.1"
                     /translation="MKGKVYLVGAGPGDPELLTLKAMRLLRTADAVLYDDLVSPEILA
                     LISPTAQVHNVGKRAGTKKIRQEEINFLMIALADSGLNVIRLKSGDPLIFGRAGEEIE
                     ALRRANIAHEVVPGVTSALGAAAATQIPLTHRRASSALVFITAQQAEGSEAANWAKLS
                     GSGATLVLYMPGQNYGELASKLQSAGLSGEIPCAIISRATTPFQQTHRTTIDDLHRAP
                     HLPAPNLLVVGEVVRFADPAVVAQDFAVPASSDSAASALLADAHSPVRTLEANKEPGN
                     "
     gene            complement(8274..>8571)
                     /locus_tag="VEJ00_10585"
     CDS             complement(8274..>8571)
                     /locus_tag="VEJ00_10585"
                     /inference="COORDINATES: protein motif:HMM:NF026174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01652.1"
                     /translation="RRGDLQIAISTSGQSPSLAQKIRQQLEKQFGPGYAAWVAELGET
                     RRLILASELDKERKLELLHSLASRQAVEAALAQAGEAAAPELSAPALVTQGDRA"
BASE COUNT         1645 a         2453 c         2719 g         1754 t
ORIGIN      
        1 ctgtttgaaa actccttccg agacgtactc gtagagggca ccgttcgggg ggatggcgca
       61 agaggggaaa cacggcgtgc caggaaaggg agcgttggcg ttgatggtcg ccagttgatc
      121 gaacccccgg gaatgcaggt atgtgaggga caaggtcgac gttttggtca gctgccgttc
      181 tacactgacc gcagcctgca acgttcccgg cgcgcggaat cggggactgg tcttgtaggt
      241 caccgaagaa ctgtctgtcg cgccgcaatt ggaaaaattg gcaagcggct gatcaacctg
      301 ggtgaaacag gttggattgt tcacggtaaa ttgccgctgc gtagttccgt tcaaacgctg
      361 ggcctgcaga atgttgctca gcgcgaagcg atcgtagaaa attccggccc cgccgcggat
      421 tacgaccttg ggcgggccgc tccggccgcc gactccccag gcaatgccca cccgtggtgc
      481 ccaatcggca tgatcctgaa tgtagttctg cgattcgaat cgcaatccgc cgctcagcgt
      541 aatgttagga cgaacgcgcc agtcgtcctg gatatagagg ccagcgtcaa acgtgaggac
      601 atcggcggtg ggaaggccgg tggtgtagcg gagttgcgtc gccaagcccg atgcggggtt
      661 ctgtagtgca tactgcagcg agatggggca gggcggattg gggtgctcgc ccgccgtgaa
      721 gttttggcaa tccgcgggcg tgccggcacc cgtgttggga tcggtttgca gcaatgaaaa
      781 tgtaaaactg ccgttgaacc cggaggtcga aaaattcgcc tcgcggttgg cgcgcaaacg
      841 gcctccgaac tttatggtct ggtttccgtg aatcacggac gtatagttct ggagttcgta
      901 gtgatcctga tggtcattgc tgagggttcc gctgccgccg ccgctgaagg cgccgggcac
      961 gccaacgctg ggatttgtgt ccgcgggagt ttgaaagttc agttcgcgca aatattgaaa
     1021 gcgcgtctca ttgaccactt tggcgccgta aatctgggtg tcgccgatct ggatcgtgtg
     1081 ttcgatgttc ttcgagttat agccctgcga cgggagggta aactggccga ggccatcgtt
     1141 ggtttccgta tcgcgccaat attgataacg cgcggtcagc gtgttgttct tggtgagggc
     1201 ccagtccaaa cgtgggccta tgttcgtgcg ctgatgcggg aaaggcaccg actcggcaat
     1261 cgggaccaga gcaaatgtcg ttgcatcaag tgtgtcggct gtaatcgccg agaggtcgtt
     1321 gatgttgcgg cgctggaagc tgaagaaaaa cgaagcgttt ttgttgaccg gcccgctgac
     1381 gctgccgctg tactgctcgg aatcgtagcc cggttcggcc ccgccaaaag gattggaact
     1441 gttgaaggca gagtcgttgc cgttgatctg gaactctccg tgaaacttgt ccgtgccggg
     1501 cttggtgaag atctcaatgc ggccgtagcc cagcttgtcg aactccgagg aaaacggatt
     1561 ctggttgatg cggatttcgc gaatcgacga cttgggcgga agctggccag cggtaaagcc
     1621 gtcgatatac atctgtccgc cgctgggccc cgccgacggt ccggcgaggg cctggagatc
     1681 ttgcagcaac tcatcggggt catcgggcag cgcttcgagt tccttgcccg agatcacgat
     1741 cgcgcccgca ttgctggccg gattcacgtc aatcgtaggc gcggtatccg agacctggac
     1801 cttttgcgtc tccacctgga ttgtcatcga aatgttaagt cgcaagggct ggtcagcgat
     1861 catcacgctg tcgttttcgt agagagtgaa tccctgggcc acaacggtga gcgcgtattt
     1921 cccagccgcc aggtccttga attcatacat cccctgagaa ttcgtcgtgg cagtgagggg
     1981 cgatccggtt gccggcgtca tgacggcagt cgcgccggag atggctgcgc ctgagggatc
     2041 agtcacctgc ccacgaagcg ctccgctggc attttgcgcc gatattgcgg cagagctgca
     2101 gagcagggaa agaaatagga ttgcccaagt tacatgtttc gtgtgcatga ctccgaaaat
     2161 ccgttgacca gtaaaatcct tttgcgatga atgatgcctt cgatgcgaga tccaggctta
     2221 tggattgccg ttgtctcctg acggcgcccc acccagattc cacggtgtca gcatgagagc
     2281 ctggccggcg cttggggcgg cctgcaggat aggctcgacc ccagcgagca gcttggtgat
     2341 tacatcgccg tcccgccctt ccgcggcgac gatcatcacc gcatcgccct tatgcaagtc
     2401 gcttaagccc acagcgggca tgcggcttag gacccgattg agatcgaacc ggccgaggcg
     2461 tggacctcct gctgaaccgc ttccgcccgg cgacatgccg ccgggcgctg ccggcccttc
     2521 tccccccgcg gatacaggcg ccggattcga tcccgaagcg ggtcggtcgg ctgccccacc
     2581 cttgaagccc aggaccagac gtgccgcctc ggcggggagt ttgcgcagct gagaaccggc
     2641 gttcactttc acttgcaacg acttcttgct caacacatcc tgaacgtcga tcgtgccgga
     2701 agttgcatcc acggaattga tggtgccggc gatattgcga aacgtaccga agacgacttc
     2761 ctccgccgtg agttcggagc cgtcggcgct gcgatcaccg cgcgcgtgga actggtcgcc
     2821 ggtccgcatt tcagacaaag aactcggctt ggcgtcggcg aagtttgccg accccgcggg
     2881 gtagcgcagc atcactgtgg ctttggtggt gtgcacagcg atctctttcc cgccgccaaa
     2941 gccggggacc gacaaactaa tggtgccgct ggcaggatca agggctttga ccactccgtc
     3001 gacgccccgc ttctgccagt cctgaagatc ttccttctgg cgagcctcga ggtcggagcg
     3061 cttcatggcc acgatgacgg aggccgcaat cgactttgtg tcttcggaag ccgcgccgcc
     3121 taccaggatc cggtcgccca cttgcaaatc ctggaacgga atcggggtcg cgttcttaag
     3181 atctttttcg cctggagcca accgcagaac acgcgtgccc ggctggacgg tcaccgtgat
     3241 ctcagagccg gaatccggcg ccagggctat ggtgcttccg ttgatcgctt tgatcgcacc
     3301 aatgcggcga gtcgcggcgc tttgaccgct cgcgctctgc gcaggagtct gcgctgcaag
     3361 aagcgcgggc gccaacagca gctcagcggt gtgagcggcg atggccgacg tcagcaggat
     3421 gaagggcacg atgaatttgc gtttggtgct cataccgccc cagagtttaa tgtacatcgc
     3481 cgccagcgcc tgaactcgct gatttacaaa gttttacccg ccagaaacac ttattaacag
     3541 aagcgccggg gggcgactgt tttgtgcggg atcggccatc ccggcaccca gagagccaat
     3601 agacgagcac ccatcccgct tctacgattt ttatgagcat attcaggttt gggatgagtg
     3661 tgtctatatc acgttgtctt ctttgctggg cgatggtggt gctggttccc atctcggtac
     3721 tgggacagac tccatcggca attcttcatg cccagggggg cgtgtgggtg aacggctacg
     3781 agggaaagga ttccaccgcg atctttgccg gcgatctgct cgaaaccaag ccggagttct
     3841 cggcgaaact tactctggaa gggtcggagg cgctgattca ggcggaatcg gtcgtcaagt
     3901 ttcaagggga tctgttggcc ctggatcacg gtggagtggc tgtcggaact tcccgcggtt
     3961 tgaaggtgaa ggtgaattgc atcaccgtcg ttcctgtcag gaatgagtgg actcagtacg
     4021 aagtcgtcga cgtgaatgga acggttcagg tggcagcgcg caagggcgac gtcaatgtcg
     4081 agcatgaaat gggcaaagga aagcccgagc ttggcgccga gggatcgcgc ggaggcacgg
     4141 ttcatgaagg cgagcaaaag agctacagcg aatcagaact atgtggagca ccgccaggac
     4201 ctacgggagg cagcagcggc ctgaacccga aatatattgg gatcggcggc gcagtcggcg
     4261 gcggcatcct gctgtgcgtg attctctgcc gcggcggtgg cagtacgaag acccagatca
     4321 gcccggataa gccctgatcc ggcccccact acagaattct ttcgtgcgtg tagcccgccg
     4381 agagcaacgc tgacaaaagt tccgcaatgt gatcggggcc gcgagtctcc atggtgatgt
     4441 cgatagcggt gtcgcccaga ttgacgccgt gataggcgcg gtcgtaggcc gtttcaacga
     4501 tgttggcgcg atggtcggcc agaattccag tgaggcggtg cagcgcgccc ggatagtcgg
     4561 gcaggtgaac gcgcaaacgg acgagccgcc catccttgac caggccgcgc tcgatgatgc
     4621 gggccagcag ggtcacgtcg atgttgccgc cgcaaaccag aacagcaacc cgctggcctg
     4681 ccaagggcaa cttgcggttg aggaccgcgg ccagcgcggc cgctccggcg ccctcggcca
     4741 gcgtcttctc acgctccagc agcaatagaa tcgcgtttgc gatttcttct tcttccaccg
     4801 tggcaatctc atccacgtac ttctgaacca gcggaaaggt ccgctcgccg gcgcggcgca
     4861 ccgcaatgcc gtcggcgatc gtggtagcag agcgcagcgc caccggcttg ccagcggcaa
     4921 tggcagcctt catcgaggga atgcgggccg gctgaacgcc gtagaccttg accgcgggat
     4981 tcatttcctt ggccgcgcag gcgatgcccc cgatcagccc gcctccgccg ataggaacga
     5041 cgatcgcctc aatatcaggc tgctgctcca gaatctccaa tcccagagtt ccctgccctg
     5101 cgatgacggc ttcgtcgtca aaagcgtgaa tgagcgtgag gtggtcccgg gcgccgtttt
     5161 cgacagcctt ttcgtaggcc tcgtcgtaat tggcgccttc gagaatgacc tcagcgccgt
     5221 aggcgcgagt ggaggaaacc ttggtcagcg gagtaggcag cggcatgaag atgcgggcac
     5281 gaatcgagtg gcggccggcg tggtaagcga caccctgcgc gtggttgccg gcggaggcag
     5341 cgatcacgcc gcgcttccgc tcttcctggc tgagtgtcag caacttgttc agcgcacccc
     5401 tttccttgaa agcgcccgtg cgctgctggt tatcgagctt caggaaaatg gaattgccgg
     5461 tgaggttgga gaaagtctcg gagtgcgtgc acggagagac gcgaatatct ccgcggatcc
     5521 tatccagagc ggcccggaca tcggaaagat tcaccatgag atcaacgcag ccatcttaca
     5581 tggagcgctc gggggagtgg gacaacgcct gatcaggaat tgtgatgcga atcacgcaaa
     5641 tttcagaaca gcgttttggc tttgcatatc ttcaggaatt gctcgtcgag tccgctataa
     5701 tctggctcaa acactcattc agatcaagcc gtcatggatt tcgatcaact catcacgttt
     5761 ctggaggtgg cgaagcaggg aagcttctcc cgcgccgggg aaaaagtgtt ccgctcgcaa
     5821 tcggcggtga gcgcgcaaat ccggcaactg gaacaggaat acggcgaccg gctgctcgac
     5881 cgctcgggca aaacggttaa gctgactcct gccggccagg tcttttttga atacgcccag
     5941 cggctgcgga gcctgcgcga cgaatcgctg accgccgtag ctgaccacag cggcactcca
     6001 cggggcacgc tgcgcgtggg ggcaaacgag gcgacctgcc tttacgttct gccggaggtg
     6061 ttcggcgaat attgccggcg ctatcctccg gtgcagatca gcatctaccg caacttcagc
     6121 tacaagatcg tcgagaagct ggaaaacgga gcggttgatg tgggcattct caccctgccg
     6181 attcaatccc ccagcctgaa gattcagccc atttttcgtg acaagctggt ggtgatggtc
     6241 agtcccaaga atccgctcgc gaagcataaa gtggtgccgg tcagcgagat cgtgaagtac
     6301 ccgctgttgt ttcccaagac cgggcacacg cggcggatgt tggataagct gtttcggccg
     6361 taccaggccc aggtgcaggt caggatggag ctgccgagca tcgggatgat caaaagcttt
     6421 gtcgcggcgg atctgggagt gtcgctgatc agcgcttcgt tcgcgcgcga cgaagtcgac
     6481 gcgggccggg taaagctgat cgacctggag gacgccgaat tgtaccgcga actggcgctg
     6541 gcctacaggc gcgatcggac gttatcagtc gctgccactg ctttcgtatc tatgctgcgg
     6601 cacatggcca tcccctcaaa gaagaccgac gcatagctgg taaggccacc gctcagtcgc
     6661 cttctgcccg gcgggaggtt gcttccggcg ccggctcgat catgtgcagg ccgcactctt
     6721 tcttggcaaa tcccggccac cgtccggccc gcggatcttc tcctggcctg acggcgcgcg
     6781 tgcaatgcgt gcagccgatg ctgggataat ccatatcgtg gagctggttg tagggcacgt
     6841 cgtggtcgtg gatatagcgc cagacctgct ttgagttcca atccacaatc gggttgacct
     6901 tcaccaggcc gaacttggcg tcccactcca cccggtgcgc tccggcgcga tgggaggtct
     6961 gatcgcgccg gatgctggta acccacgcgt ccaacgtctt gagcttgagg cggagcggct
     7021 cgaccttgcg cagattgcag cagaggtcag gatcgcggct ccacagcgct tcgccgtgaa
     7081 tgcgctcctg ctcctccggc gagaggacgg acatgacctt ctcgatcgtg atgccgtact
     7141 tctcctcgat ccggtccatc aggttgtagg tctcggggaa aagaaattcg gtgtctatag
     7201 tgaaaaggcg gaaattcttg tggacgcggg aggcgatgtc aatcatgacc atgccttcgg
     7261 cgccgaacgc ggaagaaatc gcgatgcggt tgccgaaggt ttcgaacgcc caggccagaa
     7321 cccggtgcgg gctccaggtt tcagcggcgc tctgcacttc cgcgacggca agctgaccgg
     7381 ccagttgagg agtgagggga gcctgaagct ggctctggct ttcgaacccg cttgtgatct
     7441 gatctttcaa tttcctggtt ccttgttcgc ttctaaggtt cgaactggcg agtgagcatc
     7501 agccaacagt gccgatgcag cactgtcgct gctggctggc acggcgaaat cctgcgcgac
     7561 cacggccgga tcggcgaagc gcacaacttc acccacgacc agcagattgg gagcgggtaa
     7621 atgcggtgcg cggtgtaagt cgtcgattgt ggttcgatgc gtttgctgga acggggtcgt
     7681 agcgcgcgaa atgatggcgc agggaatctc gcccgaaaga ccggcggact gcagcttgga
     7741 ggcaagttcg ccgtagttct ggccgggcat gtagagaacc agcgtcgcgc cgcttccgga
     7801 aagcttggcc cagtttgcgg cttcgctacc ttcggcttgc tgggccgtga tgaacaccag
     7861 cgcggatgag gcgcggcgat gggttagcgg gatttgggtt gccgctgccg cccctaaggc
     7921 cgaagtcacg ccaggaacaa cttcgtgcgc gatgttcgcg cgacgcagag cttcgatttc
     7981 ttctcccgcc cgtccaaaga tcagcgggtc gccactcttc aggcgtatga cgttcagtcc
     8041 cgagtcggcg agggcgatca tcaggaagtt gatttcttcc tgccggatct ttttagtgcc
     8101 ggcgcgtttg ccaacgttgt ggacctgcgc ggtgggtgaa atgagcgcga ggatctccgg
     8161 cgagacaagg tcgtcgtaca gaaccgcgtc ggccgtacgc aacaggcgca tcgccttcag
     8221 cgtgagcaac tccggatcgc ccggaccggc gccaaccaga tagactttgc ccttcatgcg
     8281 cggtctcctt gcgtgacgag cgccggtgcg gagagttccg gcgcagctgc ttctccggct
     8341 tgggcgagag ccgcttcaac cgcttgccgg ctggcgagcg aatgcagcaa ctcgagcttg
     8401 cgctctttgt ccagttcgct ggcgagaatc aggcggcgcg tttcgccgag ctccgcgacc
     8461 cacgcggcgt atcccgggcc aaactgcttt tcgagttgct ggcgaatttt ctgcgccagg
     8521 gaggggcttt gcccagaggt cgaaatcgcg atttgcaggt ccccacggcg c
//
LOCUS       DASPUL010000442         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_353288, whole genome shotgun sequence.
ACCESSION   DASPUL010000442 DASPUL010000000
VERSION     DASPUL010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353288"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VEJ00_10590"
     CDS             <1..543
                     /locus_tag="VEJ00_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXX01653.1"
                     /translation="DVMLPGGDGRKVLRRIRLTSQVPVIMLTARGDENDRIAGLEGGA
                     DDYLPKPFNPRELVARMRAVLRRKGDRALPAVFAIGDLRIDCIQRRVVRDGCEVALTG
                     SEFDILLLLVRSAGKVLSREEIAEVALGRPIGFFDRSIDNHISNLRKKLGTHVGEVER
                     IQNVRGAGYVYAGEIVEEHR"
     gene            540..1916
                     /locus_tag="VEJ00_10595"
     CDS             540..1916
                     /locus_tag="VEJ00_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXX01654.1"
                     /translation="MIGARFHSIYVKIFLWFILASGVMSGLVFLTTVVTHAPSLGASW
                     MIGVLDQYARSAVEIYALQGKAGLIEYLEKIEDISRLQATLLDPQGRDLSGRGVPSGA
                     EEVLAEARATGGTQVHIRVLWTGATVVDGPGGKYILVAKVLIPYGGLVSGALQVTVLQ
                     WLMPALPGALLCLILSRHIASPIRTLQSVAGKIADGDFSVRASPAIAPRRDEIADLAR
                     DFDRMADRIQGLLRKQQALLGDISHELRSPLTRLNVSLELVRRGESDGVKRMQSDLDR
                     LDDLIEQVLTLTRLETRSDLKRETAVNLRVILESVAEDARFEGKEEGKSVIVSRADDC
                     WLKRDPALLRSCIENVVRNAVHYTKPQTDVVISLTRTTEGGLPAARIIVSDRGAGVPE
                     EALPRLFEPFYRVTEARGHQTGGTGLGLSIAQRIAIVDGGGIRARNREAGGLEVEISL
                     PLNDGEAS"
     gene            complement(1935..2426)
                     /locus_tag="VEJ00_10600"
     CDS             complement(1935..2426)
                     /locus_tag="VEJ00_10600"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HXX01655.1"
                     /translation="MSANIALQEFNQMKDTEYQKQTEPSTLLRWCRFNFVGGIGIGVQ
                     FVALFLLKSVLHIHYLAATAIAVEAAVVHNFVWHEQFTWADRTQPGGIMLGSQQSWRR
                     SWRRFLRFNLTNGAVSILGNLALMRVMVGQGHMNYLVANAIAITLCSLANFLVSNEWV
                     FAE"
     gene            complement(2423..>3113)
                     /locus_tag="VEJ00_10605"
     CDS             complement(2423..>3113)
                     /locus_tag="VEJ00_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXX01656.1"
                     /translation="LLEMRRKRPFPDMLRVQESWTAAPEKRALLWLAARMPERIGPDH
                     LTILGLAGQIGAGACYALAPSNKLWLLGVIACLGVNWLGDSLDGTLARVRQRLRPRYG
                     FYVDHMVDTFGALALMSGLALSGCMHPWIAAGLLLAFLMLSIQSYLATHALGEFRISF
                     WRFGPTELRILLAVGNLALLWKPMVNLFGEPYRLFDVGGTIGLVGMGAMVVVFTLQNT
                     MRLYREERIPQ"
BASE COUNT          619 a          909 c         1010 g          575 t
ORIGIN      
        1 gacgtgatgc tgccgggcgg agatggccgc aaagtgctgc ggcggatccg cctgacctcg
       61 caggtgccgg tcattatgct caccgctcgc ggcgacgaaa acgatcgcat tgccgggctt
      121 gagggcggcg ccgacgatta cctgcccaag cccttcaatc cccgcgaact ggtggcgcgc
      181 atgcgtgccg tgctccggcg caagggcgac cgggcgctgc cggcggtttt tgcgattggc
      241 gatcttcgca tcgactgcat tcaaagaagg gtcgttcgcg acggctgtga ggtggccctg
      301 acaggatccg agttcgacat tctcttgctg ctggtccgct cggcgggcaa ggttctctcc
      361 cgcgaggaaa tcgcggaagt cgccttaggc aggccgatcg gcttcttcga ccgcagcatc
      421 gacaaccaca tcagcaatct gcgcaagaag ctcggcacgc atgtcggcga agtcgagcgc
      481 atccagaacg tgcgcggagc aggctacgtt tacgccggcg aaatcgtcga ggagcacaga
      541 tgatcggcgc acggtttcac agcatctacg tcaagatttt tctctggttc attctggcct
      601 cgggggtcat gagcggcctg gtgtttctca ctaccgtcgt cacccacgcg ccttccctcg
      661 gagcgagctg gatgatcggc gtgctcgatc agtacgcacg cagcgcggtc gaaatctacg
      721 cgctacaagg aaaggcaggg ttgattgagt atctagagaa gatcgaagac atttcgcgcc
      781 tgcaggcgac tttgctggac cctcaaggtc gcgacctgag tgggcgcggc gttccctcgg
      841 gcgcggagga agttcttgcc gaagcccgag ccacaggggg aacgcaagtt catatccgcg
      901 tgctgtggac gggtgcgacc gtggtggacg gtcccggcgg aaaatacatt ctggtcgcga
      961 aggtgctgat tccctacggt gggctggtga gcggggctct ccaggttacg gtattgcagt
     1021 ggcttatgcc ggccttgccc ggagccctgc tgtgcctgat tctgtcacgc cacattgcgt
     1081 cgccgatccg cacgttacag agcgtggccg gcaagattgc ggacggagat ttttccgtgc
     1141 gggcttcccc ggcaattgcc ccgcggaggg acgaaatcgc cgatctggcg cgggatttcg
     1201 accgcatggc ggaccgcatc cagggactgc tgcggaagca gcaggcactg ctgggagaca
     1261 tctcgcacga gctgcgttcg ccgctgaccc gactgaatgt ttcgctcgaa ctggtccgcc
     1321 gcggcgagag cgacggcgtc aaacgaatgc agtccgatct ggaccgcctc gacgatctga
     1381 tcgagcaagt gctgacgttg acccgcctcg agacccgcag cgacctgaag cgtgagacgg
     1441 cggtaaatct gcgagtgatt ctggaaagcg tggcggagga tgcgcgtttc gagggcaagg
     1501 aagaagggaa gtcggttatt gtttcgcgcg cggacgactg ctggttgaag cgggatccgg
     1561 cgttattgcg cagttgcatc gaaaacgtgg tccggaacgc cgtgcactac acaaagccgc
     1621 aaaccgatgt cgtgatttct ctgacccgaa cgacggaagg cggtttgcct gcagcccgaa
     1681 tcatcgtttc ggaccggggc gccggcgtgc ctgaagaggc tctgccacgc ctgtttgagc
     1741 ctttctaccg cgttacagaa gcgcgcggtc accagacggg tggaaccggc ctgggtttgt
     1801 cgatcgcgca gaggatcgcg atcgtcgatg gcggcggcat tcgcgcccgc aatcgcgagg
     1861 ccggcggcct ggaagtggag atcagccttc cgctgaacga tggggaagcg tcatgagtct
     1921 gtcgacgcgg ggaactactc tgcgaatacc cactcgttgc tgaccagaaa atttgccagc
     1981 gagcacagcg tgatcgcgat cgcattggcg acgagatagt tcatgtgtcc ctggcccacc
     2041 atcactcgca tcagagccag gttgccgaga atcgataccg ctccgttcgt gagattgaag
     2101 cgcagaaatc ggcgccatga ccgacgccat gactgttggg agcccagcat gattccgcca
     2161 ggctgtgtcc gatcggccca cgtgaactgc tcatgccaga cgaagttgtg gactacggcc
     2221 gcctcaaccg cgatggcggt ggctgcaagg tagtgaatgt gcaggacact ctttagcaga
     2281 aacagcgcga cgaactgcac gccgatgccg atcccgccga caaagttgaa cctgcaccag
     2341 cgcagcagag tggagggttc cgtctgtttc tggtactccg tgtccttcat ttggttgaat
     2401 tcttggagag cgatgttcgc gctcattgcg ggatcctttc ttcgcggtag agccgcatgg
     2461 tgttctgcag agtaaacacg acaaccatgg ctcccattcc caccagaccg atcgttccgc
     2521 ccacatcgaa aaggcgatac ggctcgccga aaaggttcac catcggcttc cagaggagag
     2581 ccaaattccc tacggccagc aggatgcgca attcggttgg gccgaagcgc cagaaggaaa
     2641 tgcggaactc gcccagcgcg tgcgttgcca gataggactg gatcgagagc atgaggaagg
     2701 cgaggagcag gccggcggcg atccagggat gcatgcagcc tgacagggcc aggccgctca
     2761 taagtgccag agcgccaaag gtatcaacca tgtggtcgac gtagaacccg tagcgtgggc
     2821 ggaggcgctg gcgaacccgc gccagcgtgc cgtcgagaga atcgcccagc caattgacgc
     2881 caaggcaggc gatcacaccg agcaaccaga gcttgtttga gggggccagc gcgtagcagg
     2941 cgccggcacc gatctggccg gcaagaccaa gaatcgtcag gtgatccggg ccgatgcgtt
     3001 ccggcatacg cgctgccagc cataggagcg cacgcttttc tggcgcagcg gtccagcttt
     3061 cctgcacgcg cagcatgtca ggaaacgggc gcttgcggcg catctccagc aac
//
LOCUS       DASPUL010000443         7610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_761371, whole genome shotgun sequence.
ACCESSION   DASPUL010000443 DASPUL010000000
VERSION     DASPUL010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761371"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1997)
                     /locus_tag="VEJ00_10610"
     CDS             complement(<1..1997)
                     /locus_tag="VEJ00_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXX01657.1"
                     /translation="MIGQNISHYRVIEKLGGGGMGVVYKAEDTRLRRFVALKFLPEDV
                     TRDAAALSRFRREAQAASALNHPNICTIYDIGEDGDQAFIVMEYLEGETLKHVIGDRP
                     LEIEKVLSIGIDVADALDAAHSKGIVHRDIKPANIFVTRRGAAKILDFGLAKVTQEAS
                     RTLDPSEATRTEGVSAEHLTSPGSALGTVAYMSPEQVRGKPLDARTDLFSFGIVLYEM
                     ATGAVPFRGETSGVIFDAIMNRAPVAPVRLNPNLPPKLEDVINRALEKDRDLRYQHAV
                     DIKSELLRLKRDLDSARSGSAITPASSSDAHIAAPPEPLPTAAQKTSGSARVEAAASS
                     GSVTPSRASAPRTSLKWTIPGAVVLVLALVAGAIYLRRKPAVQLTDKDQLILADFNNQ
                     TGDPVFDSTLKEALAIQLEQSPLIQLVSDAELHDSLQYLGQPKNQHITPDLAQQIGQR
                     LGVKAYLAGTITPIGQAYVISINAVNCATGDVFGREQVTAPDKSAVLAAVSSAATAMR
                     ARLGESLASIQKLSTPYLSSVTTASLQAFHAFSLGEDEHRMGRDFPEAASFYEQAVQL
                     DPSFAMGWARLGVVYGNSGSSSKAQEYMKKAYDLRERVTERERMYIEAQHALQGYDLP
                     KALESYELYASTYPRDANALNNLGSVLALEGNNEQAATAFV"
     gene            2221..2589
                     /locus_tag="VEJ00_10615"
     CDS             2221..2589
                     /locus_tag="VEJ00_10615"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXX01658.1"
                     /translation="MVEYIQTGDLDDIFSALADPTRRAILQSLAHGPATINEIARPFA
                     VSLNAISKHVMVLERAGLLRREIQGREHHCRINGRPLRDANTWLEHYREFWDLRLDAL
                     EVYVDRKFKAAKREAHGKVR"
     gene            2573..3037
                     /locus_tag="VEJ00_10620"
     CDS             2573..3037
                     /locus_tag="VEJ00_10620"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXX01659.1"
                     /translation="MEKSADGKHRVVARRRMPAPREIVYDAWIDPEGLRQWMCPGDVI
                     SAEATLDVRVGGSFRITMKTATKVHEHVGTYQVVDRPSKLVFTWSGLENPAEITLVTV
                     DFIPHGDESELVITHERFTDPAVAQRYEMGWGTIEKKFAEHLAKAASKPRSR"
     gene            3119..3538
                     /locus_tag="VEJ00_10625"
     CDS             3119..3538
                     /locus_tag="VEJ00_10625"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HXX01660.1"
                     /translation="MQEERNKEVIRKFTSIFKNQHNVDGIDHLFDPGFTHHFRDPLPP
                     GLAGLKQIGRMMNGAFPDVVVTEEDLVAGGNKVVERSSAVATHKGSLMGEKPTNQPIH
                     WTEIHIYRLENGRICEHWVEMAMMELLQQIGALPKAA"
     gene            3624..3929
                     /locus_tag="VEJ00_10630"
     CDS             3624..3929
                     /locus_tag="VEJ00_10630"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HXX01661.1"
                     /translation="MPIYQTGGYRVKPSAVPRVKQAIKDFVAYVQGNEPGSQMYLAWQ
                     QKDDPTVFLHLFIFADADAQTRHGQSDAVKRFEAIYSPELLDGEVVFTDYEMIAGKR"
     gene            4084..4329
                     /locus_tag="VEJ00_10635"
     CDS             4084..4329
                     /locus_tag="VEJ00_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01662.1"
                     /translation="MFVGLFETYKNPDEEWIGTELTFDLKPENASTVVLFAQRGWKEQ
                     IEFMHYCSTKWAVYLLSLKSLCETGIGRPFPDDLDIG"
     gene            4499..5170
                     /locus_tag="VEJ00_10640"
     CDS             4499..5170
                     /locus_tag="VEJ00_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01663.1"
                     /translation="MHGRSCACLLTLIALVAAPAALCGQNRPDSNRSDAPREDPPGPL
                     GYTTQLPIVEPVFPEPVFPQPGSPGRWPRPSTPVSFGLPQLTRSAGTIFSGTVTGIVR
                     RPAARGPGVETVEVTFRVENAIRGTTQGATLTFYQWIALWSAGQRYRVGERLLLFLYP
                     PSKLGLTSCVAGPMGRFAVDPLGRIGLTAQHLAAFRRDPVLGGKSRVSLRDFALAVSQ
                     ATEEE"
     gene            5167..>7610
                     /locus_tag="VEJ00_10645"
     CDS             5167..>7610
                     /locus_tag="VEJ00_10645"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HXX01664.1"
                     /translation="MNFNELTMKTCVAPTVRSFLLRLALAAVLVFVFSLASRAGGPKY
                     VAGTSYFDSSMSGQPLVWPQGQITYYTDQGDLSPILDNAAANDLVSTSFAQWTSVHTA
                     AVVAANGGQLAEDVNGTNVTENADGTISMPSDIQSSATDKPVGVVYDYDGSVTNALLG
                     QGAGDASQCFFNAVYGGVDNFGATAAYQHALLVLNGQCAQASSQLVDVEYRLVRVIGT
                     VLGVGWSQVNLNVATGAPPPTANDFAGFPVMHYLDLTACVPITRCYPNPFQLAADDVA
                     AISRLYPVTAQNQSNFPASQVFASSTARIHGSVWFTDSQGNRTTPMQGVNVVARWIDP
                     ATGLPSRRYAASSVSGFLFTGDAGNPVTGFNDAFGNPLSNFGSSSPTVEGFFDLAGLQ
                     LPKGSSAQYQLSAEPIDRTWSLGVAPYDPYQVAPSGSFQPVLLTVQAGQDVEQDILMT
                     SSAQPLPTATPDTWTSPVPVEPAGDWMGSLSSYGDVAYFFLPAQANRTLSVAVTALDE
                     SGRASEAKLQPVIGMWAASDPQGTAPPASTPSPFNTVLPGMTRLDAAVATSAKFIVGI
                     ADLRGDGRPDYHYHAQVLYADSVTPSRIGVTGGIVTLQGTGFSPGLTVTVGSMPATPL
                     AISAGQMIVAAPAQPDGPQSITVANPATGGSSTMTNGLTYGAAASDNIVLLSGTNPPT
                     PVGAQAVHPVIVQVLAADGVTPVSGATIGWSANNGLQLSACNATPSCSVTTDQSGYAS
                     TWLTPTAAGAANITATLAPGVYSPAKSVTATLSATESASDIGVLASYLWISQGATVTV
                     PLTARVLSNGIPQTGV"
BASE COUNT         1495 a         2426 c         2186 g         1503 t
ORIGIN      
        1 ttcacgaacg cagtcgccgc ctgctcgtta ttcccttcaa gggcaagaac cgagcccaga
       61 ttgttcaacg cattggcgtc gcggggataa gtcgaagcat agagctcgta ggattccagg
      121 gccttcggca aatcataccc ctgcaaggca tgctgcgctt cgatgtacat ccgttcgcgc
      181 tccgtgacac gctcgcgcag atcataggcc ttcttcatat attcttgcgc cttggaggat
      241 gaacccgagt ttccgtagac cacgcccagc cgcgcccagc ccatggcaaa cgagggatcc
      301 agttgcactg cctgttcgta gaacgacgcc gcttccggaa aatctcggcc catgcgatgc
      361 tcgtcctcgc ccaaggagaa agcgtgaaac gcctgcagtg aggccgtggt cacgctgctc
      421 agatagggtg tcgacagctt ctgaatcgac gccagcgact cgcccagccg cgctcgcatc
      481 gccgtggctg cgctggaaac tgcggccagc accgccgact tgtcgggcgc ggtgacctgt
      541 tcgcgtccaa acacgtctcc ggtggcgcag ttcaccgcat tgatggagat gacgtatgcc
      601 tgtccgatcg gtgtaatcgt tcccgccagg taggccttca cgccaagcct ctgcccgatc
      661 tgctgcgcca ggtccggagt aatgtgctga ttcttgggct gcccgagata ctgcaggctg
      721 tcgtgcagct ctgcgtcgct gaccagctgg atcagcggcg attgctccag ctgaatcgcc
      781 agcgcttcct tgagtgtgga atcgaaaacg ggatcgccgg tctgattgtt gaagtcggca
      841 agaatcagct gatctttgtc ggtcagctgc actgccggtt tcctgcgcaa gtagatcgca
      901 ccagcaacga gcgccaacac cagcacaacc gccccgggaa tcgtccactt gagcgaggtg
      961 cgtggagcgc tcgccctcga cggagtgacg ctgcccgagg acgccgccgc ttccacccga
     1021 gccgatccgc tcgttttctg tgccgccgtg ggaagcggtt cgggcggagc ggcaatatgc
     1081 gcatcggagg aagacgcagg cgtgatcgca cttccgctgc gcgccgaatc cagatcccgc
     1141 ttcagccgca gcaactccga cttgatgtcg accgcgtgct ggtagcgcag gtcgcggtct
     1201 ttttcgaggg cccggttgat cacgtcttcg agcttcggcg gcaaattcgg attgagccgc
     1261 accggcgcca ccggcgcccg gttcatgatc gcgtcgaaga tgactccgga cgtctccccg
     1321 cggaacggca ccgccccggt tgccatctcg tagagcacga tgccgaacga aaacagatca
     1381 gtacgcgcat cgagcggttt gccccgcacc tgctccggcg acatgtaggc gacggtccca
     1441 agcgcggatc cggggctggt caaatgttcc gcgctcacgc cttcagtcct tgtagcctcc
     1501 gacggatcga gcgtgcggct ggcttcctgc gtgactttgg ccaagccaaa gtcgagaatc
     1561 ttggccgctc cgcgtcgggt cacgaaaatg ttcgccggct tgatgtcccg atgcacgatg
     1621 cccttggagt gcgcggcatc tagcgcgtcg gccacgtcaa tcccgatcga cagcaccttt
     1681 tcgatttcga gcggccggtc gccgatcacg tgcttcagcg tctcgccttc cagatactcc
     1741 atgacaatga aggcctggtc gccatcttcg ccgatgtcgt agatcgtgca gatattggga
     1801 tggttcaggg ctgacgccgc ctgcgcctcg cgccggaagc ggctgagcgc cgcagcgtcg
     1861 cgcgttacat cctcaggaag gaatttcagg gctacaaagc gccgcagccg cgtgtcttca
     1921 gccttgtaaa cgactcccat ccctccgccg cccagcttct cgatcacacg gtagtgagag
     1981 atgttctgac cgatcatggc gcgcggccct cgaggccgca ttacgtgctg agaatacggg
     2041 gttcagcgct tgcggcgcct tcctcggtct cgcggattca ggcaagccat cttaggtttg
     2101 gggagaaaca atgtcaacga gcggagacct ggcgtgagtc gtgtgtccgg caattgtctg
     2161 aatatttttt gacaaagaaa gtgttgacaa gccggatagg tctccctaat attcaaccga
     2221 atggttgaat atattcaaac cggcgacctc gatgacatct tctctgccct cgccgatccg
     2281 acgcggcgtg ccattctgca gtcgctcgcg cacgggcccg ccacgatcaa cgaaatcgcc
     2341 cggcccttcg ccgtttcgtt gaacgccata tcaaagcatg tgatggtact ggagcgcgcg
     2401 ggattgttgc ggcgggaaat ccagggccgc gaacaccatt gccggatcaa tggccgcccc
     2461 ttgcgcgacg ccaacacctg gctggaacac taccgcgagt tctgggactt gcgcctggac
     2521 gctctggaag tctatgtcga ccgaaagttc aaagccgcaa agagggaagc gcatggaaaa
     2581 gtccgctgat ggcaagcatc gcgtcgtcgc ccgccgccgc atgccagccc cacgtgagat
     2641 cgtctacgac gcctggattg accccgaggg tctgcggcag tggatgtgcc ccggcgatgt
     2701 gatctcagcc gaagcgacgc tcgacgttcg cgtgggcggc tcgttccgca tcaccatgaa
     2761 gaccgcgacc aaggtgcatg agcacgtggg aacctaccag gtcgtcgacc gcccgtccaa
     2821 actggtcttc acctggtcgg gtctggagaa tcccgcagag atcacgctgg tcacggttga
     2881 cttcatcccg cacggggacg aaagcgaact cgtgatcact catgaacgct tcaccgaccc
     2941 ggcggtggct cagcgttacg aaatgggttg ggggacgatc gagaagaagt ttgccgagca
     3001 tttggcgaaa gccgcgtcaa aaccccgaag tagataggca gcaggatttc aaggagcatg
     3061 aggatgtgct tctaacgagg tatcggcgcg ttcgaatcaa cgacctgaaa ggactggtat
     3121 gcaagaggaa cgaaacaagg aagttatccg gaaattcacc agcattttca agaaccagca
     3181 caacgtcgac gggatcgatc atctgtttga tccaggcttc actcaccact tccgcgatcc
     3241 cttgccgccg ggcctcgccg gactgaagca gatcggccgc atgatgaacg gagccttccc
     3301 cgatgtagtc gtcacggaag aagaccttgt cgcgggcgga aacaaagtcg tcgagagaag
     3361 ctcggctgtc gcgacccaca aaggttccct gatgggtgag aagcccacga accagcccat
     3421 ccactggacc gagattcaca tctaccgtct ggaaaatggc cgaatctgcg agcactgggt
     3481 cgagatggcc atgatggaac tgttacagca gatcggagcc ttgcccaagg cagcgtaagt
     3541 cgatccgcaa ggcttcgcac gttccctgcc gtgcctcgac agctgccgcg atgcagcacg
     3601 aactgtttga gaggagattt cgaatgccca tctaccaaac tggtggatat cgagtgaaac
     3661 cgtcagccgt acccagggtc aaacaggcaa tcaaagactt cgtcgcctac gtccagggaa
     3721 acgagccggg gtcacagatg tatctagcct ggcagcagaa ggacgatcca accgtttttc
     3781 tgcatctctt catcttcgcg gacgccgacg ctcagacacg gcacggacaa tccgatgcgg
     3841 tgaagcgctt cgaggcaatc tactctcctg aactgctgga cggcgaagta gtgttcaccg
     3901 actatgaaat gatcgccggc aaacgctaga ccctcggccc cctgcatggc atgagctcga
     3961 cgctcggatc agaaaggaat tcgtgtgtat ggcaacaacc ggacaggttg taaaggactt
     4021 ctacgccgca gtcgtaaacc gggacctcgc ttcagctcgc gagtatttgg tagacgatct
     4081 cgtgtgttcg tcggcttgtt tgagacttac aaaaatccag atgaggaatg gatcggcacg
     4141 gagctgacct tcgatctgaa acctgaaaac gcctctacag tcgttctatt tgcgcagcga
     4201 ggctggaaag agcagatcga gtttatgcac tactgcagca cgaagtgggc agtttacctg
     4261 ttgagcctca agtcgttgtg cgaaacaggg atcggtcgcc cgtttcccga cgacctcgac
     4321 atcggataag gaaaatctcg agaggtgaac gcaggtaccg cccgtttgat ccaaaatctc
     4381 gacagggaat cacagcctga tgtgatatcc gtcactgatt cccttggcct cttgaaccta
     4441 ggatcgacaa gtttcccggc aagccctccg agcagacact acaccgtgag gtttcttcat
     4501 gcatgggaga agttgtgctt gtctgctcac gcttattgcg ctggtggccg cgcctgctgc
     4561 cctttgcggc cagaatcgcc ctgattcgaa tcgctcggac gcaccgcgcg aggatccgcc
     4621 tgggccgctg ggctacacaa ctcaacttcc cattgtggaa ccagtctttc ccgagccagt
     4681 ctttcctcag cccggctctc cgggacgctg gccccggcca agcactccgg tctcattcgg
     4741 gctcccgcaa ctcacccgct cggccggcac gatcttctcc ggaaccgtca ccggcatcgt
     4801 ccggcgtccg gctgctcgcg gcccaggagt ggagactgtc gaagtcacct ttcgagtcga
     4861 gaatgccatc cgaggtacaa cgcaaggcgc gaccctgact ttctaccaat ggatcgcgtt
     4921 gtggtccgcc ggccagcgtt atcgggtggg cgaacgtctg ctgctttttc tctaccctcc
     4981 gagcaagctt ggacttacca gctgcgtggc cggaccgatg ggccgcttcg cagtcgatcc
     5041 tttgggccgg atcgggctga cggcacaaca tcttgcagct tttcggaggg atcccgtcct
     5101 cggtgggaag tcgcgcgtaa gtctccgcga tttcgccttg gcggtgagtc aggccactga
     5161 ggaggaatga acttcaacga gttgaccatg aaaacctgcg tggcacctac cgttcgctcg
     5221 ttcctcctca ggctggccct ggccgccgtg cttgtgttcg tcttttccct cgcttcgcgg
     5281 gccggaggtc cgaagtacgt cgccggcacc agttacttcg actccagcat gagcggccaa
     5341 ccactggtgt ggcctcaggg ccagatcact tactacaccg accagggcga tctcagcccg
     5401 attctcgata acgctgccgc caacgacctc gtttcaacgt ccttcgccca gtggacttcg
     5461 gttcatacgg ccgccgttgt ggccgccaac ggtggacaac tagccgaaga tgtgaacggc
     5521 acgaatgtca ccgagaacgc tgacggcacg atctcaatgc cgagtgacat ccagtcttcc
     5581 gccaccgaca agccggtggg tgtggtctac gattacgacg gctcggtaac gaatgcgctg
     5641 ctcggccagg gagccggcga cgccagccaa tgtttcttca atgccgttta cggaggcgtg
     5701 gataatttcg gcgcaaccgc cgcctaccag cacgccttgc tcgtcctcaa tggccagtgt
     5761 gcgcaggcat cctcgcaact tgtcgatgtc gaataccgcc tggtgcgggt gatcggcacc
     5821 gtactcggcg tcggctggtc gcaggtcaac ctcaacgtcg cgaccggggc gcccccgccc
     5881 actgccaacg acttcgccgg attccccgtc atgcactacc tagacctcac agcctgcgtg
     5941 cccatcacgc ggtgttatcc gaatccgttc cagctcgctg cggatgacgt cgccgccatc
     6001 tcgcggctct atccggtcac cgcccaaaat caatccaact tccccgccag ccaggtcttt
     6061 gcttcatcga cagcccgaat ccatggctcg gtatggttca cagattcaca gggaaatcgc
     6121 actacgccca tgcagggagt gaacgtcgtg gcccgctgga tcgatccggc gaccgggcta
     6181 ccctcccgcc gctacgccgc ttcctcggtg tcgggattcc ttttcaccgg cgacgcaggc
     6241 aatccggtca ctggcttcaa cgatgcgttc ggcaaccctc tgagcaattt cggctcttcc
     6301 agcccaaccg tcgagggatt ctttgatctc gccggattgc agctgcccaa aggaagcagc
     6361 gcccaatatc agctgagcgc ggagcccatc gatcggacat ggtcgctagg agtcgctccc
     6421 tacgacccgt atcaggtcgc gccttccggc tctttccaac ccgttctcct gacagttcaa
     6481 gccggccagg atgtcgagca ggacattctc atgacctcca gcgcccagcc gctgccgacg
     6541 gccacaccgg acacctggac cagtccggtt cccgtcgagc cggctggcga ctggatggga
     6601 tcgctcagca gctatggcga tgtggcgtac ttctttttgc ccgcccaggc gaaccgcaca
     6661 ttgtcggttg ctgtcacggc gctcgatgaa tccggccgag ccagcgaggc caagctccag
     6721 cccgtgatcg ggatgtgggc cgcctccgat ccgcaaggta cagcgcctcc agcctcgact
     6781 ccgtcaccgt tcaacacggt cctgccggga atgacgcgcc tcgacgccgc cgtggccact
     6841 tctgctaaat tcatcgtcgg aatcgccgac ctgcgcgggg acggacgtcc cgactaccac
     6901 tatcacgccc aggtgctcta tgccgactct gtaaccccat ctcgcatcgg agtcactggt
     6961 ggcatagtta cgttgcaggg cacgggcttt tccccgggac ttacggtcac cgtaggcagc
     7021 atgccggcga cgcctctcgc catcagcgcc gggcagatga tcgtcgctgc ccccgcgcaa
     7081 cctgacggcc cgcagagcat taccgtcgcg aatcccgcga ctggcggatc gtccacaatg
     7141 accaacggac tgacctacgg cgccgccgcc agcgacaata tcgttctgct tagcggaacc
     7201 aaccctccca ctccagtggg cgcgcaggcg gtccatcccg tgatcgtgca ggttctggcg
     7261 gccgacggcg tcacgccggt tagcggcgca accatcggct ggagcgcaaa caacggcctg
     7321 cagctctcag cttgcaacgc cactccttcc tgttcggtca cgacggatca gagcgggtac
     7381 gcatccacat ggcttacgcc taccgccgcc ggagccgcga atatcacggc gacactggct
     7441 ccgggcgtct acagtccggc caaatcggtc acggcgacgc tgagcgccac cgagtcggcg
     7501 tctgacatcg gtgtgctggc ctcgtacttg tggatctccc agggagccac ggtaaccgtg
     7561 cccttaaccg ctcgtgtcct gagcaacggt atcccgcaga cgggcgtgaa
//
LOCUS       DASPUL010000444         4361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2003439, whole genome shotgun sequence.
ACCESSION   DASPUL010000444 DASPUL010000000
VERSION     DASPUL010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2003439"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2726)
                     /locus_tag="VEJ00_10650"
     CDS             complement(<1..2726)
                     /locus_tag="VEJ00_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXX01665.1"
                     /translation="MSLLRIAEPILDAEERFEGFENLMPFKVHNILLVSSLYDSFILR
                     EDGRLNELLIDESLELSLQQIPGITHVSSCAEAVDLAKSNPQFNLIVTNLAVGDMDAA
                     QLAREVRRAGLDVPVVVLAYDYRGIKNFVARNPLTDIDRIFLWQGNARILIAIVKYVE
                     DKRNVLHDTRAMGVPVLLMVEDNIRYYSSFLPVIYTELIKQSRRVIQEGINVAHKLVR
                     MQARPKILLASNFEEAAQLVQAYRDYLFGLVSDVEFPWEGGLNPDAGFELARMVKSMV
                     PDVPVVLQTSRTEFRPRAHAEGYSFLRKRSPTLLRDLRRILTDEFGFGDFVFRLADQS
                     EVGRATDLNELEEQLQTVPVESIVYHAQSNHFSHWLMARTEFALAAKLRPRKVSDFPS
                     PEHLRRDLIASINDYRREQNEVLIADFKADTFKAQESSFLRIGDGSLGGKARGLAFVR
                     HLLRTRRITRRFSGVRIAVPPAVVLATDMFDLFLSENNLSDFALHCDDDNEIQQKFLD
                     APLPAPLRENLKAYLEEVRYPLAVRSSSLLEDSQYQPFTGVYETFMLGNQQASLETRL
                     AELSEAIKRVYASTFSQHAKAYVRATPYRLEEEKMAVILQQVVGTAHETRFYPDFSGV
                     VRSHNFYPVEPMTFSDGIAAVALGLGRTVVDGGKCLAFCPRYPRNLLQFSSVEDILAN
                     TQSEFCALELDGIPHGGGPGHLREAHFTLDVAEGDGTLQAVASTYSADNHAVYDGLSR
                     PGTRVVTFAPMLKHGVFPLATILDVLVRAGEDALGNPVEIEFAVRLPRSEEPAEFGFL
                     QIRPLTLARDHQDLTIGEVDPAQLICQSTKVLGNGRIENLRDVVVVDSHRFERSRSQE
                     VARAVAHFNAKLGAENRPYLLIGVGRWGSNDPWLGIPVEWDEISGARAIVEAG"
     gene            complement(3547..>4361)
                     /locus_tag="VEJ00_10655"
     CDS             complement(3547..>4361)
                     /locus_tag="VEJ00_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HXX01666.1"
                     /translation="LRNEFSGVLTGKGLNWGGSLIRPEATGYGCVYFADEMLKTRKDK
                     LEGKLCLVSGSGNVSQYTIEKLLDFGAKPVTVSDSNGVIYDPDGIDREKLAWVMELKN
                     VKRGRIRDYADHFKKAIFMPTDIKLDHNPLWNVKGDCAFPSATQNEITAKDAANLIKN
                     GVKLVAEGANMPSTLEATKMFLDAKVLYGPAKAANAGGVATSGLEMAQNSARLSWTRE
                     EVDDRLHKIMIAIHQTCFETAEKYGTPGNLVNGANIGGFVKVANAMLDQGLV"
BASE COUNT          857 a         1356 c         1291 g          857 t
ORIGIN      
        1 ccggcctcga cgatcgcccg cgctcccgaa atttcgtccc actccaccgg aatgccgagc
       61 cagggatcgt tcgatcccca gcgtcccacg ccaatcagca gatacggacg attctccgcg
      121 cccagcttgg cattgaaatg cgcgactgcc cgcgccactt cctggctgcg gctgcgctcg
      181 aagcgatgcg agtccaccac caccacgtcg cgcagatttt cgatgcgccc gttgcccaaa
      241 accttcgtgc tctggcagat cagctgggcg ggatccactt ctccgatggt caaatcctga
      301 tgatcccgtg ccaacgtcag agggcggatc tgcaggaatc cgaactccgc cggctcttcg
      361 ctacgcggca gccgcaccgc gaactcgatc tccaccggat tgccgagcgc gtcttctccc
      421 gcgcgtacca gcacgtcgag aatcgtggcc agcgggaaga cgccgtgctt gagcatgggc
      481 gcaaacgtta ccacccgcgt ccctggccgg ctgagcccgt catagacggc gtgattgtcg
      541 gcggagtaag tagaggcaac ggcctgcagc gttccatcac cctccgccac atcgagggtg
      601 aaatgcgcct cgcgcaagtg accagggcct ccaccgtgtg ggatcccgtc caattccagc
      661 gcacagaact cgctctgcgt gttagccagg atgtcttcga ccgaagagaa ctgcagcagg
      721 tttcgcggat aacgcgggca gaacgccagg cacttgccgc catcgactac agtgcgcccc
      781 agccccagcg ctactgccgc gatcccgtcg gaaaaggtca tgggctcgac gggataaaaa
      841 ttatgcgagc gcaccacacc cgagaaatcg ggatagaagc gggtttcgtg agccgtaccc
      901 acaacctgct gcaagatcac cgccatcttc tcttcttcca gccgatatgg cgtggcccgc
      961 acgtaggcct ttgcatgctg gctgaacgtc gaggcgtaca cgcgctttat ggcctccgac
     1021 agctccgcca gccgcgtctc gaggctggcc tgctggttgc ccagcatgaa cgtctcatac
     1081 acgccggtga agggttgata ctgcgagtcc tcgagcaggc tggacgaacg cacggccagc
     1141 gggtagcgga cttcttcgag ataggctttc aggttttccc gcagcggcgc gggaagcgga
     1201 gcgtccagaa atttctgctg gatttcgttg tcgtcatcgc aatgcagggc aaaatcgctg
     1261 agattgttct cgctcaggaa cagatcgaac atgtcggtgg cgagcacgac cgcgggcggc
     1321 accgcgatgc gcactccgga aaaacgccgc gtgatccggc gggtgcgcag cagatgtcgc
     1381 acgaaggcca gtccgcgcgc cttgcctccg agcgagccgt cgccgattcg cagaaagctc
     1441 gattcctggg ccttgaacgt gtcggccttg aaatcagcga tcagcacttc attctgctcc
     1501 cgccggtaat cgttgatgga agcgatcagg tcgcgccgca ggtgctccgg gctgggaaag
     1561 tcggagactt tgcgtggtcg cagcttggcc gccagtgcaa actccgtgcg cgccatcagc
     1621 cagtgcgaga agtgattgct ctgcgcgtgg tagacgatgc tttccacggg cacggtctgc
     1681 agctgttcct cgagctcgtt cagatcggtg gcgcgtccca cctcgctctg atccgccagg
     1741 cgaaagacga agtcgccgaa tccgaactcg tcggtcagaa tgcggcgcag atcccttaac
     1801 aacgtgggcg agcgcttgcg caagaacgaa tacccttccg cgtgcgcccg cggacgaaac
     1861 tccgtgcggc tggtctgcag caccacaggc acgtcgggaa ccatgctctt gaccatgcgc
     1921 gccagttcga agccggcgtc gggattgagg ccgccctccc agggaaactc cacgtccgaa
     1981 accaggccga acaggtagtc gcggtaggcc tgcacgagct gcgcggcctc ttcgaagttc
     2041 gaggccagca agatcttggg ccgcgcctgc atacgtacca gcttgtgcgc cacgttgatg
     2101 ccttcctgaa tcacgcgccg cgactgcttg atcagctcgg tgtagatgac gggcagaaac
     2161 gaggagtagt agcggatgtt gtcttccacc atcagcagca cgggcacgcc catggcccgc
     2221 gtgtcgtgta gcacgttgcg cttgtcctcg acgtacttga cgatcgcaat caggatgcgg
     2281 gcgttgccct gccacagaaa gatgcggtca atgtcggtca agggattgcg cgcaacgaag
     2341 ttcttgatcc cgcgatagtc gtaagccagc accaccaccg gcacgtcgag cccggcacgg
     2401 cgcacttcgc gcgccagctg cgcggcgtcc atgtcgccca cggcgaggtt ggtgacgatc
     2461 agattgaact gcggattcga tttcgccaga tcgaccgcct cggcgcaact cgagacatgc
     2521 gtgatgccgg gaatctgctg caggctcagc tcaagcgatt cgtcgatgag cagttcgttg
     2581 agccgcccat cttcgcgcag gatgaaggag tcgtacaagc tcgacaccag aagaatgttg
     2641 tgcaccttga agggcatcag gttctcgaag ccttcaaagc gctcttccgc gtcaaggatc
     2701 ggctcggcga tgcgcagcag ggacatgaag ttgttaagag ctaatcatag cgtcgagcac
     2761 aagacaacta tgtatgcggg atcactcgtc cgagggaggt catgtaggga cagccgcctc
     2821 ggctgtccag tcgagcgaag ctcgacggcg ctgtcgaatc gatccaggag ctgggatttg
     2881 ctgcccgctc gcaggaatgc cccgggcgga ggtcctgcgg acagcagaga cgagatcggt
     2941 gaccaaccgg gcacgagatc cgccccatgc taaacatgcc ccagcggccg ccaaatcgat
     3001 ttaaccgcta tatctatgta ctgcacaatc aagtacttac atacgcattc ttgcacaggg
     3061 acatgaagca gtaaaacgcg gccacgcaag agcttccaga gcactgcact aaagctggtg
     3121 cagtgaacag aatggttgcc cgcgtctcag aagaacggag tcgttttgct ggccaggcgg
     3181 aaagacggga atgtcccgca tcgatctcac gggcctttat ggcgccgtcg tgaattccgt
     3241 cagctttctc agcttcaccc cggcgtcact agcggcatcc tgctgcagcc atcccagatt
     3301 gacattccta ctattgcgaa aaacaaaaag acgtttcgca tgattgtcct cgatcgcagt
     3361 acggatcagt gagcaaagtt cccgggttgt agccagcagc gaatgcactg cgctcatgtc
     3421 ggccgtggga attctcggcc gctccgctgt tccaaaaaat caaaatcccc acctttgccg
     3481 cacagaacgc ggcaaagatg gggcacccat ttcgtcgtta cttcacccga ccttcaaccg
     3541 aacgttctac acgagaccct gatccagcat ggcgttcgcg actttcacga agccgccgat
     3601 gttggcgccg ttcaccaggt tgccgggcgt gccgtatttc tcggccgttt cgaagcaggt
     3661 ctggtggatg gcgatcatga tcttgtgcag acggtcgtcg acttcctcgc gggtccagct
     3721 cagccgggcg ctgttctggg ccatttcgag gcctgacgtg gccacgccgc ccgcgttcgc
     3781 ggctttggcc ggtccgtata gaaccttggc gtcgaggaac atcttggtcg cttcgagcgt
     3841 ggacggcatg ttcgcgccct cggccaccag cttgactccg ttcttgatca ggttcgccgc
     3901 atctttcgcg gtgatttcat tctgcgtggc gctcgggaag gcgcaatcgc ccttcacgtt
     3961 ccacagtggg ttgtgatcga gtttgatgtc ggtgggcatg aagatggctt tcttgaagtg
     4021 gtcggcgtag tcgcggatgc gtccacgctt cacgttcttc agctccatga cccaggccag
     4081 cttctcgcgg tcaatgccat cgggatcgta gatcacgccg ttcgagtcgg acaccgtgac
     4141 cggcttggcg ccgaagtcga gcagcttctc gatggtgtac tgcgagacat tgccgctgcc
     4201 ggaaaccagg cagagcttgc cttccagctt gtccttccgc gtcttcagca tctcgtcggc
     4261 gaagtagacg cagccgtagc cggttgcttc cggacggatc agcgaaccac cccagttcag
     4321 gccctttccg gtgaggacgc cgctgaattc gttgcgcagc c
//
LOCUS       DASPUL010000445         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_81388, whole genome shotgun sequence.
ACCESSION   DASPUL010000445 DASPUL010000000
VERSION     DASPUL010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81388"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2480)
                     /locus_tag="VEJ00_10660"
     CDS             complement(<1..2480)
                     /locus_tag="VEJ00_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01667.1"
                     /translation="MLLLSLNLVAWPLISLTFGSAGESISVGRALGCVAGLILIPVLL
                     IIGTASLWSDLQLQSWMRVNAREYAHMISAQLRGDLIDGARILTTFRPVYSDSTVPPR
                     CKRGPELSIRPAPGQVTLGERCDTYSGIPKTPLFGYDHQKRPVDACVVHEPLDDDRCD
                     DVLLLPSRQAPVDGRWSPIRSILAVNVNGDRFGPAMSPFSTVTVSPKAKIPDREYFQA
                     LKAGQGWMLDTPPSAPLQMVAQRLYNRGDASKALQLAVPLCDTPDAFCGIITGDIRMY
                     GLTSPISPPLLKFAVIDTTTGTVIFSSTDTRSLAENFFRESEQDPALLAVVKSHHEAD
                     FIGRYLGEPQRFYYTPISNVPWGVLVFYSRKDLADLPFRAGSAALVCYLGLLLAALIA
                     LCVIRWLWLLFTATFPRPLDLIKPVWPRLRLPGLRLPEWYRDLSRFRPPAWAFLVLLI
                     GMLTSLSTMATFASALAVLVAAACAGLIFKPDEKKGDSKRSPAQQYSQCLFTLITAVS
                     ILPAFALFLEFYHLQYEALIRDGLAENAAQIQSRYDAVHEELHRLLPPTLGGGKGADA
                     NSPGPWSLALHPNIGMSDQDHDLPSVVQIQLVTGVPQPLSPRNPNLHQRLVWQWTASS
                     PDQQRRLAIITEPATTVGPEDMQCGLEEKSRKDTCIVRMSDGNQLEFQSSARLGGTLF
                     ANEFEWNWLRILAVFGAVLFAAMGTSFLCWLVTTRLIGLTEQLKRLSTVKPDDPGGRS
                     FDDKWKGLGEPEQLALYQIARGELINPRNFAVLEKPMREGLIVLDPFPVLSSSDLKDL
                     ILRRESESGLERLQREAGYSPWRTIRGPL"
BASE COUNT          532 a          718 c          785 g          460 t
ORIGIN      
        1 agtgggccgc ggatcgtcct ccaggggctg tacccggcct cgcgctgcaa cctctcgagg
       61 cccgactcac tctcgcgacg aagaatcaaa tccttcaagt cagacgatga gagtaccggg
      121 aagggatcca gaacaatcag accctcgcgc atcggtttct cgagcacggc gaaattgcgt
      181 ggattgatca gctcgccacg ggcaatctga tatagggcga gctgctcggg ttcaccgagg
      241 cctttccatt tgtcatcgaa gctcctgccg ccgggatcgt ctggcttcac cgtggacagg
      301 cgtttcaact gctccgtcag gcctatgagc cgagtcgtga ccagccagca caagaatgag
      361 gttcccatcg cagcgaacag cacagctccg aagacggcca gtattcgtag ccaattccac
      421 tcgaactcgt ttgcaaagag ggtgccgcca agtcgcgcac tggattgaaa ttccagttga
      481 ttgccatcgg acatgcgcac gatgcaggta tctttccggc tcttttcctc gagcccgcat
      541 tgcatatctt ccggcccgac ggtggtcgcc ggctccgtga tgatcgcgag tcgacgctgc
      601 tgatccggcg agcttgcggt ccattgccaa acgagccgtt gatgcaggtt agggttccga
      661 gggctcagag gctgcggtac gccggtgacg agctgtattt gcacgacgct gggaagatca
      721 tgatcctgat ccgacatgcc gatgttgggg tgcaaggcca ggctccacgg cccaggagag
      781 ttcgcatctg cgcccttccc cccgccaagg gttggcggca agagacggtg gagctcctca
      841 tgcacggcgt cgtaacgtga ctgaatctgc gcggcgtttt ccgccaatcc atcgcggatc
      901 agcgcctcgt actgaaggtg gtaaaactcg aggaacaggg cgaatgcggg aagaatggat
      961 acggcggtga tgagcgtgaa caggcactgt gaatattgct gggcaggaga tcgtttcgaa
     1021 tcaccctttt tttcgtctgg cttgaagatc agaccggcac aggcggccgc caccaggaca
     1081 gccagggcgc tcgcaaaggt cgccatcgtc gagagcgatg tgagcatgcc gatcagcaag
     1141 accaagaagg cccatgcagg tggcctgaag cgactcagat cgcgatacca ctcgggcaag
     1201 cgcagtccgg gcaagcgcag tctcggccac accggtttga tcaggtccag cggccgggga
     1261 aacgttgcgg tgaacagaag ccagagccac cggatcacgc acagcgcgat cagcgcggcg
     1321 agcagcaggc cgagataaca gaccagggcg gcggagccgg ctcgaaacgg cagatctgcg
     1381 aggtccttcc tggagtagaa taccaacacg ccccagggca cgttgctgat gggtgtgtag
     1441 taaaagcgtt gcggctcacc gaggtatctg ccaatgaagt cggcctcgtg gtgcgatttg
     1501 acgacggcca gtagtgccgg atcctgctcg gattcgcgaa agaagttttc agccaggctc
     1561 ctcgtgtccg ttgagctgaa tatgactgtg ccggtcgtgg tatcgatcac ggcaaacttg
     1621 agaagcgggg gagaaatcgg cgacgtcagc ccatacatcc ggatgtcgcc agtgatgatc
     1681 ccgcaaaagg catcgggcgt atcgcaaagg ggaacggcca gctgcagcgc tttggacgca
     1741 tcccctcggt tgtacaatcg ctgcgccacc atctgtaagg gagcggatgg aggcgtgtcc
     1801 agcatccaac cctggccggc tttcagtgcc tgaaagtatt cccggtccgg aatcttggcc
     1861 ttcgggctga cggtcacggt actgaagggg ctcatggccg ggccgaagcg gtcaccgttc
     1921 acattcacgg caaggatgct tcgaatcggc gaccagcggc cgtcgactgg ggcctgccgg
     1981 gacggcaaca gaagaacgtc atcgcagcgg tcgtcgtcga ggggctcatg cactacacaa
     2041 gcgtcgacgg gccgcttttg gtggtcgtac ccgaaaagcg gcgttttagg gattcccgaa
     2101 taggtatcac accgttctcc gagtgtgacc tgtcctggcg ccgggcggat cgacagctcg
     2161 gggccgcgtt tgcagcgcgg aggaactgtc gaatcgctgt agacgggccg gaacgtggtg
     2221 agaattctgg ctccatcgat cagatccccc cgcagctggg cgctgatcat atgggcgtat
     2281 tccctggcgt tgacccgcat ccagctctgt agctgcagat cactccaaag ggaggcagtg
     2341 ccgatgatca acaggactgg aatcaggatc agaccggcaa cacagccgag cgctcttccg
     2401 acggagatcg attccccggc agaaccgaag gttaaactga tgagcggcca cgccaccagg
     2461 ttcagactga ggagaagcac ggcagcccag aagcc
//
LOCUS       DASPUL010000446         4223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_492722, whole genome shotgun sequence.
ACCESSION   DASPUL010000446 DASPUL010000000
VERSION     DASPUL010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492722"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VEJ00_10665"
     CDS             <1..318
                     /locus_tag="VEJ00_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01668.1"
                     /translation="AISTDDEGVARSDMTHEYLRAVQGYKLSYATLKAMTRQSLEHSF
                     LPGDSLWVTTKGAFRPAAACAADAPGVAKPSAACAKFLAANERAREQWKLEKELSTFE
                     KKF"
     gene            complement(383..2827)
                     /locus_tag="VEJ00_10670"
     CDS             complement(383..2827)
                     /locus_tag="VEJ00_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX01669.1"
                     /translation="MSMLQDLKCGVRGLTKSPGFAALAIVTLALGIGANTTIFSWINS
                     TLLNPVPGLTNPSEVVSLTLSKPGENPFPFTYPDVEAMREGQKSFTGIIACNFVQISL
                     TGKRKPERLSGMVASANYFDVLGVRPFLGRGFLPADDDKPGGAPVAVISYRLWQTHFA
                     ADPNIVGQMVELNQHPYSIVGVTPAAFQGSQTGVRTDIWVPIMMEAQLNPLGDLLRDH
                     HYFWLFAFGRLKPGVSLAQAQDEMTRLLKREVDNYPEEHKGHRAVSVYPLWQNPFGLN
                     FVMAKLLPTLMCIAGLVLLLACANVANLMLVRSVGRRREIAIRISMGGSRWRLVRQLL
                     VESLLLSLAGGVVALLFTLWTQGTLMKFLPITPDIPLLLTVEADRTVLLTTLAISVLT
                     GVIFGILPALRASGVAPADVLKEESGSMSGGLGKARLASALVVGQISLSLLLLVCAGL
                     FIRSFRSAREIDPGFNPHNVLIASYDLFTAGYDDATGTEFHRQLLAKLETLPGLQSVA
                     LSSRVPLTFAGGSTAVKPEGYVSPANESMETQVAIVSPNYFHTMQIPMAKGRDFVPQD
                     TKSSQRVVIVSEAFANRYWPRQEALGKQLNSDLTHEWFTVVGVARDCKEVSLNENPLP
                     FLYLPVSQVYRPGMIINARAAGDPLALARSVENAAHELNPDLALFDETTVELRDQFSS
                     FGQRVAGTFVGAFGLLALALAAIGIYGVTAYTTRQRTHEIGIRVTLGATRRDVLRLVL
                     GQGLRLMVIGVGVGLVLAFALTRFLASLLLGVTSTDALTFSTVALLLCCVTLFACFIP
                     GFWAMRFDPMVALRQQ"
     gene            3201..3800
                     /locus_tag="VEJ00_10675"
     CDS             3201..3800
                     /locus_tag="VEJ00_10675"
                     /inference="COORDINATES: protein motif:HMM:NF014752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HXX01670.1"
                     /translation="MGRKRTLIPMPLTWADQPVPVPPPLLPVVAERGGTQLRTPLPVA
                     IVDTREQNPLSFRRFKGWFARVEHRALRLGDYSIQGMENSCIVERKDLADLIQSFTSN
                     RAVFVDRLRRMSELPHSLLVVTASLSEIKSEYPYRAANPNRITQSLLAVLAGLRVPFI
                     CTETHELGEEIVASYLYQTFLYDWLDRNGHGCYLADGDL"
BASE COUNT          883 a         1361 c         1174 g          805 t
ORIGIN      
        1 gccatctcga ccgacgacga aggcgtcgcc cgctccgaca tgacgcacga atacttgcgc
       61 gccgtccagg gatacaagct ttcctacgcg acgttgaagg ccatgacgcg ccagagcctc
      121 gaacacagct tcctgcccgg agacagcctg tgggtgacaa cgaaaggagc gttccgaccg
      181 gctgccgcct gtgctgcaga cgctcccggc gtggccaagc cctccgccgc ctgcgcgaag
      241 tttctcgccg ccaacgagcg cgcccgcgaa cagtggaagc tggagaaaga actctccacc
      301 tttgagaaga agttctaggc aggacgccaa cccgttccat gagcaacttc ctcgaaaggg
      361 agctcgctac ggctggtaca gcctattgtt gccgcaaggc caccatcggg tcgaatcgca
      421 ttgcccagaa cccgggtatg aagcaggcaa acaaagttac acagcaaagc aggagcgcaa
      481 ccgttgaaaa cgtcagggcg tcggtgctgg taacgccgag cagcagacta gccaggaaac
      541 gcgtcagggc gaaagccagc acgagtccca cccctacacc gatgaccatc aaccttagcc
      601 cctggccgag caccaggcgc aggacgtctc gtctggtggc gcccagcgtc acgcggatgc
      661 caatctcatg ggttcgttgc cgcgtggtgt aggccgtcac tccgtaaatt ccgatggcgg
      721 ccagcgccaa agccagcagt ccgaaggcgc cgacaaacgt tcctgcgacg cgctgcccga
      781 agctggagaa ctggtcccgc aactcgaccg tagtctcatc gaacagcgca aggtcgggat
      841 tcaattcatg tgccgcattt tccaccgatc tggccagagc caacggatcg cccgccgcgc
      901 gcgcgttgat gatcatgcca ggccggtaaa cctgggatac cggcaaatac aggaacggca
      961 gcgggttttc gttcaggcta acctctttgc aatcccgcgc aacgccgacc accgtaaacc
     1021 actcgtgagt caggtccgaa ttgagctgct tgcccaaggc ttcctgccgt ggccaatacc
     1081 ggttggcaaa tgcctcgctg acgatcacca cccgctgcga actcttggtg tcctggggaa
     1141 cgaaatcgcg tcctttcgcc atgggaattt gcatcgtatg aaaataattc ggcgagacga
     1201 tcgcgacttg cgtctccatc gactcattcg ccggagaaac atacccctcg ggctttactg
     1261 cggttgagcc gccggcaaac gtgagcggca cgcggcttga cagggccacc gactgtagcc
     1321 ccggcagggt ttccagtttg gcgagaagct ggcgatgaaa ttcagtccca gtcgcatcgt
     1381 cgtatccggc ggtgaacaaa tcgtaggagg cgatcagtac gttgtgtgga ttgaaccccg
     1441 gatcaatttc ccgcgcgctt ctgaagctgc gaataaacaa tcctgcgcag accagcagca
     1501 gcaacgaaag cgagatttgt cccactacca gcgcgctggc caggcgcgcc ttgcccagcc
     1561 cgcccgacat gctcccgctt tcttccttca gaacatcggc gggagctacg ccggaagcac
     1621 gcagcgccgg cagaatccca aagatcacgc cggtcagcac cgaaattgcc agtgtagtca
     1681 ggagcaccgt gcgatcggcc tcgacggtga gcaacagcgg gatgtccggc gttatcggca
     1741 ggaatttcat caacgttccc tgggtccaga gcgtgaacag caatgccaca acgcctcccg
     1801 ccagcgaaag cagcaggctt tctaccagca gttgccgcac tagtcgccat cggctcccac
     1861 ccatggagat gcgaattgcg atctcgcgcc ggcgaccgac tgaccggacc agcatcagat
     1921 tggcaacatt cgcacatgcc agcagcagaa ccaggcccgc gatacacatc agcgttggca
     1981 gtagcttcgc catgacgaaa ttcaggccga atggattctg ccagagcggg tatacgctca
     2041 cagccctgtg tcccttgtgc tcctccggat aattgtcaac ctcgcgcttc agcagccgcg
     2101 tcatttcgtc ctgtgcctgc gccagcgaaa cgccgggctt cagtcgtccg aacgcaaaca
     2161 accagaagta gtgatggtca cgcagcaggt cacccaaagg attgagttgt gcttccatca
     2221 tgattgggac ccaaatgtcg gtccgtaccc cggtctggct tccctgaaac gctgcgggcg
     2281 tcacccccac gatggaatat gggtgctggt tgagttcaac catctggccg acgatgttcg
     2341 ggtccgctgc gaaatgcgtc tgccataggc gataactgat tactgccacc ggcgcgccgc
     2401 ccggtttgtc gtcgtcagcc gggaggaaac cgcgtcccag aaatggccgc acgccaagca
     2461 catcaaaata atttgctgaa gctaccatgc ccgagaggcg ctccggtttt ctcttgccgg
     2521 tcagactgat ttgaacgaag ttgcacgcga tgatcccggt aaaactcttc tgcccctcgc
     2581 gcatcgcttc cacatccgga taggtaaacg ggaaagggtt ctcgccgggc ttgcttagcg
     2641 taagcgacac aacttcactg ggattggtga gcccgggaac gggattcaac agtgtcgagt
     2701 taatccagct gaagatagtg gtatttgccc cgattcccaa ggcgagcgtg acgatggcaa
     2761 gcgcggcaaa cccagggctt ttcgtcagtc cccgcactcc gcatttgagg tcttgtagca
     2821 tgctcatgct cgcctccttt ggctcgactg cgcgtccgac gtcgggatcg tgtgccgctt
     2881 cagagcgtgc tgagctgatt cccttttgcc ggaggcagca cgtcctgctc cagttaccgg
     2941 atctaaaacg ccaaagaaaa cactggagtt tagtctattc cggacaggtc gaaggaagct
     3001 cttctgttcg tttccgctta cgacctgtcc gaaaatgaac aggcgatgtt gttcgagcct
     3061 ctcggagcac aaaacagcgg gcacgacttc cacccatgcc gatctctgca gagaggattt
     3121 agcaccaagc aaattggtga atcaggaccc agcgcctgtc agctacactt acaagcggcc
     3181 cacctgaggg aaatcacacc atggggcgca aacgaactct catcccgatg ccactcacgt
     3241 gggccgatca gccggtgcca gtcccgccgc cgcttctgcc tgtcgtggcc gagcgcggcg
     3301 gcacgcaact gcgcacgccc ctgccggtcg ccatcgtcga cacgcgtgag cagaatccgc
     3361 tctccttccg ccgcttcaaa ggatggtttg cccgcgtcga acaccgcgcc ctgcgcctcg
     3421 gcgactattc gatccagggc atggagaact cctgcatcgt cgagcgcaaa gaccttgccg
     3481 acctgatcca gtcgttcacc agcaatcgtg ccgtctttgt cgatcgcctg cgccgcatga
     3541 gcgaactgcc gcacagcctg ctcgtcgtca ctgcgtcgct gagcgaaatc aagtccgaat
     3601 atccctaccg cgccgcgaat cccaaccgga tcacccagtc cctgctcgca gtcctggccg
     3661 gtctccgcgt gccgttcatc tgcacggaga cgcacgaact cggcgaggag atcgtcgcct
     3721 cgtacctcta ccagacattt ctgtatgatt ggctggaccg caacggccac ggctgctacc
     3781 tcgccgacgg cgacctgtga cctagatttc aggaggcttg ggcgccccat ttctcgcgcg
     3841 ttctttgcgc gagaagtggg gatttcgcgg gttgccccac gcttggcagg tcttttgcga
     3901 gaggttggaa caagaggggg acaacccgta cgatgggaac aaccgtggaa gagtggcgct
     3961 ttagcgccgc gttgagcgtt tagaatttcg tgggctttag cccccgctgg attggcgcgc
     4021 aagacacggt ttcgtcatac gtcacgccat tgacacatcg aaatgcctga cccattcacc
     4081 ctcgccgact ccgccgccaa actcctcctg tcgcgcacgc cgctgcggcc caagatcggt
     4141 ctcgtcctcg gctccggcct gggcgctttc gccgactctc tcgccgacgc caccgctgtc
     4201 ccctacgccg acatcccctc ttt
//
LOCUS       DASPUL010000447         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_81965, whole genome shotgun sequence.
ACCESSION   DASPUL010000447 DASPUL010000000
VERSION     DASPUL010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81965"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VEJ00_10680"
     CDS             <1..1186
                     /locus_tag="VEJ00_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01671.1"
                     /translation="DYFTWTLDEARAVLTEEEAKVAALHYDINEIGEMHHNPAKNVLF
                     VRAPVEEIARRMNSTPEHVRELIESAKKKMYAARLQRPTPYVDKTVYVGWNSLCVSAY
                     LEAAKVLHLDGARRFALKSLDRVLAEAWMHSSERNAGESARATHALLHVVAYSDPKAG
                     HRAVPGLLDDYAFTALACLDAYEATADLSYFKFARSIADAMIAKFFDATSGGFFDSEP
                     GAEGSALGVLATRRKPLQDSPTPAGNPMAAIALIRLHHHTGEASYRDKAEQTLETFAG
                     VAEQFGIFAATYGIAVLHFLESPTQVVVIVEDEADVAARELYAAATAPFAFNKATLRL
                     ASNQAVAGNLPPALAATIPNLPQLRSGKPFAVLCSGSACQPPVYSAAELRRALTTALQ
                     TP"
     gene            complement(1804..2715)
                     /locus_tag="VEJ00_10685"
     CDS             complement(1804..2715)
                     /locus_tag="VEJ00_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01672.1"
                     /translation="MCDNVHPGLLYYALWIAHPILQLGIAGLMMQRGLHRSFRFFFGY
                     IVTQLVTFGIIFPTYWRDGEATFYLYWICQGVSVAFGFGVIHEVFVDVFRSFHTLRDL
                     GTVLFRWAGLVMLLVAAVVSVSTNSSEIAPWMQAVITAQRCVRIIQVGMVLFLLFFAH
                     YVGVSRRQRSFGIALGFGSFAMVELVLVASWVGNHLGNPSISMVNMAAYNLSLMVWFG
                     YVAVKSPARESGATLLQTHRWEQSLADIQHPLPADSLIPMFEGMVDRALSRTKHVVPI
                     SSEREERPASAAPAGFHLPAVRVGSKP"
     gene            complement(2976..>3203)
                     /locus_tag="VEJ00_10690"
     CDS             complement(2976..>3203)
                     /locus_tag="VEJ00_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXX01673.1"
                     /translation="MAEVMTEDPLTIHTNEDIQNCLALMRRHNFRHLPVCYDGHLVGM
                     VSLRDILLHDLDEKDDEVRMMRAYIHSTPDA"
BASE COUNT          688 a          991 c          933 g          591 t
ORIGIN      
        1 cgactacttc acctggaccc tcgacgaggc ccgcgccgtc ctgaccgaag aagaggccaa
       61 agtcgctgca ctgcactacg acatcaacga aatcggtgag atgcaccaca atcccgccaa
      121 gaacgtgctg tttgtgcgcg ccccagtcga ggaaattgcg cggcgaatga actcgactcc
      181 ggaacacgtg cgtgagttga tcgagtccgc caaaaagaag atgtatgccg cgcgcctgca
      241 gcggccgacg ccgtacgtcg acaagaccgt gtacgtgggc tggaacagcc tgtgcgtttc
      301 cgcctatctg gaagcagcga aggtgctgca tctcgacggg gctcggcggt tcgccctgaa
      361 atcgctggac agggtgcttg ccgaggcgtg gatgcactct tctgaaagaa acgcgggcga
      421 gagcgcccgc gccacacacg ctttgttgca cgtcgtcgcc tattccgatc ccaaggccgg
      481 acaccgcgcc gtccctggct tgctcgacga ctacgccttc accgcgctgg cctgcctcga
      541 cgcttacgag gcaaccgccg atctcagcta cttcaagttc gcgcgctcca tcgccgacgc
      601 gatgattgcg aagtttttcg acgcgacttc cggcggattc ttcgactccg agcccggtgc
      661 cgaaggcagc gcactgggcg tccttgctac gcgccgcaag ccgctgcagg attcacccac
      721 gcctgcgggc aatcccatgg ccgcgatcgc attgatccgc ctgcatcacc acaccggcga
      781 ggcaagctat cgagacaaag ctgagcagac gctggagacc ttcgccggcg tagccgaaca
      841 gtttggaatc ttcgccgcga cctacgggat tgcggttctg cacttcctcg agagcccgac
      901 acaggttgtt gtgattgtcg aggacgaagc cgacgtcgcg gcgcgtgaac tgtatgccgc
      961 agcgaccgcc ccatttgctt tcaacaaggc cacgctccgg ctggcgtcga atcaggctgt
     1021 cgccgggaat cttccgcccg cgctggcggc gacgattccc aatcttcctc agctgcgttc
     1081 cggcaagccc ttcgccgtgc tctgctcggg ctcggcctgc cagcccccgg tatactccgc
     1141 cgcagaactc cgccgggcac tgactaccgc gctgcaaacc ccttagcccg caaaaaaaga
     1201 acgggctgac ggatcggtat gaattccgcc agcccaagga ttgcgagccc taagttcggc
     1261 ccaagttacc gcaggttact ttggttacct tgccgccccc ggctgactca gattaactac
     1321 ctgggtcacg ttggctcggc agtttttggc tttgggtggg cacagaaaaa tcgggcctcc
     1381 gtccgcggct gggagctgtt gaacggtagc tgcgacaaat gtgcccacga gtcctagcat
     1441 caggaccgta atcctgattg cacgtttcat gggttttctc ctaaacgaca cgcccgcgtg
     1501 ccccaaaaac agggcagacc gacgcggttt tggattataa ggcacccgtc aagcgatcct
     1561 cactacaatt ctgaccctcc acctgcagcc gacattgatt gccggagttc caccttaccc
     1621 cgccccctcg aaacagcgcg gcgatgtata ggcttcccct tactacctta aagtaatcgt
     1681 ttatggaagg attgtagcgc tgtgctgggc gtgtggaatt agggcgctgt tacagattca
     1741 ggataacccg atctgcggtc aggacgtctg gcagacagga cacgaggatt cggcgggcag
     1801 cagttacggc ttggatccga cgcgtacggc cggaagatga aacccggccg gggctgcaga
     1861 cgccgggcgc tcttcccgct ccgaggagat cggaaccacg tgcttggtgc gcgacagggc
     1921 gcgatccacc attccttcaa acatcgggat gagcgaatcc gccggaagcg ggtgctgaat
     1981 gtcagccaga ctctgctccc agcggtgggt ctgcagcagg gtcgccccgg attcgcgggc
     2041 cggactcttc accgcaacat agccgaacca caccatcagg ctcaggttgt aggccgccat
     2101 gttcaccatg ctgatcgagg gattgcccag gtgattgccc acccaagagg cgaccaagac
     2161 cagttccacc atggcaaacg aaccaaatcc caacgcaata ccgaaactac gctggcgccg
     2221 actgacgccg acgtaatgcg cgaagaaaag caggaacagc accatcccga cctgaatgat
     2281 gcgcacgcag cgttgcgccg tgatgacggc ctgcatccag ggtgcgatct ccgacgagtt
     2341 cgtcgaaacc gacaccacag cggctaccag tagcatgacc aggcccgccc agcggaacag
     2401 caccgtaccc aggtcgcgca aggtgtgaaa cgaccggaac acatcgacga agacttcgtg
     2461 aatgactccg aagccaaagg ccacgctgac tccctggcag atccagtaca ggtagaaggt
     2521 ggcttcgcca tcgcgccagt aggtgggaaa gatgatgccg aacgtgacga gctgggtcac
     2581 gatgtagcca aagaagaagc ggaaggagcg gtgcaggccg cgctgcatca tcaggccggc
     2641 aatgcccagc tggagaatgg gatgcgcgat ccagagcgcg taatacagaa gaccgggatg
     2701 tacgttatcg cacatcgagt gacgttcgcc tcaccagctc cggattacct tgccgaagct
     2761 cttggctcat gcgcaatctt tagttcaggg acttccagct cgcctagcta agttaacttt
     2821 ttccaacctg acgttcaatg cactgtagta atcgggagta acgttaccgt tccggtaccg
     2881 ggagggcaag gttactttcg tgctagaacg gccggaagca ggaaagacac acgcctggcg
     2941 ccgttaaagg gcaaagggcg agttcgagga gcgcttcagg cgtctggggt ggagtggata
     3001 taggcccgca tcatgcgcac ttcgtcatct ttctcatcca gatcgtgcag caggatgtcg
     3061 cgcagcgaga ccatccccac aaggtgaccg tcatagcaaa ccgggagatg ccggaagtta
     3121 tgccgccgca tcagggccag gcaattctgg atgtcttcgt tcgtgtgaat ggtgagcggg
     3181 tcctcggtca tcacctcggc cat
//
LOCUS       DASPUL010000448         3857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1858690, whole genome shotgun sequence.
ACCESSION   DASPUL010000448 DASPUL010000000
VERSION     DASPUL010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858690"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1600
                     /locus_tag="VEJ00_10695"
     CDS             80..1600
                     /locus_tag="VEJ00_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HXX01674.1"
                     /translation="MLLALAAILCVGCGFALASAGWPARPLRSADLYLRASLSVPFGL
                     AIFSVVFFLTRVLGLSNLLLADLAVFGLLLTGLALRLHWSGRAGFVGVLTKTQVDPQR
                     LEPAGIADGTSSNRTARSEATNEDRHPGWISGALTAGFVASIGVALYSAGMRLFAHPH
                     GNGWDAFAIWNLHARFLYRGGAHWRDGFSPAISWSHPDYPLLLPAAVAHFWSYVGHDD
                     AAVPAVIALLFTFCTVGLLCFGLSALRGRTAAMLGGMALLSTPAFIDVGSWQYADVPL
                     SFFFLATVVLLCFWDEADSSARSGFLALAGVSAGCAAWTKNEGLLFLCVMVSVLVWSV
                     RRERRRGVMEKNPGGAGISSLPFLATIVPAVVLIMYFKHFVAPSGDLFTDRATMLHKL
                     MDPARYWAVIKWFGMEFLRFGSWWAIPGTVLLAGLYLAVGKEDRGHQPGFRAGCVALV
                     LTLAGYFAVYLITPLDLYWHLRFSLDRLFLQVWPSALFLFFVGARMGSPSTVSQAG"
     gene            1813..2001
                     /gene="rpsU"
                     /locus_tag="VEJ00_10700"
     CDS             1813..2001
                     /gene="rpsU"
                     /locus_tag="VEJ00_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXX01675.1"
                     /translation="MRLQEGESLENALRRFKRKVQQEDIIKEVKRHSFYLKPGEKKRV
                     KAALARKRARKKTRKEQD"
     gene            2259..2576
                     /locus_tag="VEJ00_10705"
     CDS             2259..2576
                     /locus_tag="VEJ00_10705"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HXX01676.1"
                     /translation="MEFQDKVLQCVDCGGEFIFTAGEQLFFHDKQFKNEPKRCKACKA
                     KRVSVLGAAPASGQGQRFTKVETQAVCSGCGKQTTVPFRPTQGRPVFCRECFQSRRTA
                     ATA"
     gene            2649..>3857
                     /locus_tag="VEJ00_10710"
     CDS             2649..>3857
                     /locus_tag="VEJ00_10710"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXX01677.1"
                     /translation="MRYHRNVPGYYVENFGCRATQADGAALERQFEECGLARASTAAQ
                     AAVVVLNTCTVTAAADQDARAAIRRVQRQNPDARIVVTGCYAQRAPDEIAVLPGVDLV
                     VGNSHKHQLAQIALNRGAGAVLAPAFVPLESLGISGQRWDSADAPRDVFVSDIFAHTE
                     LLAAPVFDAAGERTRPNLKIQDGCDNRCSFCVIPYVRGHSRSLEGDRVIAEVNSLVEG
                     GYREVVISGINLGRWGRDLGFGLPASGFRTVAAGAEFLRKPRAGSRKPTLASLIRIIL
                     AETSLEKLRISSVEPMDWSDELITLVAESPRIAKHAHVPMQSGSDAVLRRMHRKYRPW
                     HYREKIEKIREAMATAAIGADVMVGFPGETEAEFEATRRMVEELPFTYLHVFTYSARP
                     GTPAAAMRNQV"
BASE COUNT          648 a         1047 c         1324 g          838 t
ORIGIN      
        1 cgggcttccg gcgaatctgc aactcacgag agatttcggc gacggagttc tgctgttctc
       61 taacaaggac gcccggtaga tgctgctcgc gctggcggcg attctgtgcg ttgggtgcgg
      121 gtttgccctg gcgagtgcgg gatggcctgc gcgcccgctg aggtccgctg atctttattt
      181 gagagcgtcg ctgtcagttc ccttcggtct cgctatcttt tcggttgtct tcttcctcac
      241 acgcgtgctg ggcctgtcca atcttctcct ggctgacctg gctgtattcg ggctgctgct
      301 gacggggttg gcgttgaggc tccattggag cggacgggcc ggtttcgttg gcgttctcac
      361 gaaaacgcag gtcgaccctc agcggctaga gcccgccggg attgcggacg gcacgagttc
      421 gaatcgcacg gcgcgatcgg aggcaacaaa tgaagacagg catccggggt ggattagcgg
      481 cgccttgaca gcaggatttg tggcttcaat cggtgtggcg ctctattcgg ccggcatgcg
      541 actgttcgcg catccccatg gcaacggctg ggacgcattt gccatctgga atctgcacgc
      601 gcggtttctc tatcgcgggg gagcgcattg gcgggatgga ttcagcccgg cgatttcgtg
      661 gtcgcatccg gactatccgc tgctgctgcc ggcggcggtc gcgcacttct ggagctatgt
      721 cgggcacgac gatgcggccg tgcctgcggt gatcgctctt ctgtttacct tctgcacggt
      781 cgggctgctc tgctttgggc tttctgcgct tcgtggacgc actgccgcaa tgctgggcgg
      841 catggcgttg ttgtcgacgc cggcgttcat tgacgtaggg agctggcagt atgcagatgt
      901 gccgctctcg ttcttctttc tcgcaaccgt cgtcctgctc tgcttttggg acgaagcgga
      961 ttcttcggcg cgttcgggtt ttctggcgct ggctggcgtt tctgcggggt gtgcggcctg
     1021 gaccaagaat gaggggctgc tgttcctgtg cgtcatggtt tctgttctgg tctggagcgt
     1081 taggcgcgag cgaaggcggg gagtgatgga aaagaatccg ggcggcgctg gaatatccag
     1141 cttgccgttt ctggcaacca tcgtcccggc agtggtcttg attatgtatt tcaaacattt
     1201 tgttgcgccg tcgggcgatc tgttcaccga tcgggcgacg atgctgcaca agctgatgga
     1261 tccggcacgg tactgggcgg tcatcaaatg gttcggaatg gaattcctcc gctttggaag
     1321 ctggtgggca attccgggta cggtcctgct ggctggtttg tacctggctg tgggaaagga
     1381 agaccggggg catcagcctg ggtttcgcgc cgggtgtgtg gcactggtgc tcacgcttgc
     1441 gggatacttt gcggtctatc tgatcactcc gctcgatctc tactggcatc tccgcttctc
     1501 gctggaccgg ctctttctgc aggtgtggcc cagtgccttg ttcctgttct tcgttggggc
     1561 gagaatgggg tcgccgagca cggtgagcca ggcgggttga gcatcgtccg aattgactag
     1621 tcactcgtgg tcggcggatg ggatggtggg tggcctgtcc cggctaaccg tctcaaaatg
     1681 aacatgatcc ggccgaacgg ccggaaattg cagggcagct attccggtgg tttcgcttga
     1741 cttggggtca aaaccctgca ataactattc ggtccaaacg ggacgagtct gggggtgtgt
     1801 cagttggcag aagtgaggct tcaagaggga gaatccctcg agaacgctct gcgccgcttc
     1861 aagcgcaagg tgcaacagga agatatcatc aaggaggtaa agcgccactc cttttacctg
     1921 aagcccggcg agaagaagcg cgtaaaggcc gcgctggctc gcaagcgagc ccgcaagaaa
     1981 acacgcaagg aacaggacta aatcctgaaa gagccgcctc cacccggggc ggctctgctt
     2041 aatctggccc ccgccaagtg gagtgactta ggggttcata attcatagct cttccggcat
     2101 acaagcacga tagtttgtac cacacaccgt ccgggtgcgt ggcgggccga gcacaaggga
     2161 atttacgagg ggggatgtac acaacctgag tttcttcgca ccctgccgtt tttccccacg
     2221 gcgggcgcgg ggagacgggc cgaagggagc catcctcgat ggaatttcag gacaaggtgc
     2281 tgcagtgtgt ggattgtggg ggtgagttta tcttcaccgc cggggagcaa ctgttctttc
     2341 acgacaagca attcaagaac gagcccaagc gttgcaaggc ctgcaaagcc aaacgggtct
     2401 cagtgctcgg cgcggcgccg gcgtccgggc aaggccagcg cttcaccaag gtcgaaaccc
     2461 aggcggtgtg ttcaggatgc gggaagcaga cgacggtgcc attccggcca acgcaagggc
     2521 gtccggtgtt ttgccgggag tgcttccagt cgcggcggac ggcggctacg gcctgatccc
     2581 acaggggttg tgagtgctgg ttttggtgcc cgtgtgtgac ggaccgcgcc tcgttgcggt
     2641 cccgaagggt gcgttaccat aggaacgtgc ccggctacta cgttgaaaat ttcggctgcc
     2701 gcgccacgca ggctgacggc gcggccctcg aacgccagtt cgaggagtgc ggcctggcgc
     2761 gggcttcgac tgcggcgcag gcggccgtgg tcgtgctgaa cacgtgcacg gttacggcgg
     2821 ctgcggatca ggacgcgcgg gcggccatcc gccgagtgca gcggcagaat cccgacgcca
     2881 ggatcgtggt gacaggctgc tacgcacagc gcgctcccga cgaaattgcg gtccttccgg
     2941 gggtggatct tgtcgtggga aactcgcaca agcaccagtt agcgcagatt gcgctgaatc
     3001 gcggcgcggg cgctgtgctg gctccggcct ttgttccgct ggagagtctg ggcatttcgg
     3061 gccagagatg ggattctgcc gacgcgccgc gggacgtctt cgtctccgac atttttgctc
     3121 acaccgagct gcttgcggcc ccggttttcg acgctgccgg ggagcgcacg cgcccgaatc
     3181 tcaaaatcca ggacggatgc gacaaccggt gctcgttctg cgtgattccc tacgttcgcg
     3241 ggcatagccg gtcgctggag ggagatcgcg tcatcgccga ggtgaattcg ctggttgagg
     3301 gcgggtatcg ggaagtggtc atcagcggga tcaacctggg gcggtggggg agggatctcg
     3361 gcttcgggct tccggcttcg ggcttccgga cagttgcggc cggggcagaa tttttgcgga
     3421 agccgagagc cgggagccgg aagccgacac tggcgtcgct gattcgcatc atccttgccg
     3481 agacgtcgct cgagaaattg cgcatttcgt cggttgagcc gatggattgg agcgacgagc
     3541 tgattacgct ggttgcggaa tcgccgagaa ttgccaagca cgcgcacgtg ccgatgcagt
     3601 ctggcagcga cgcggtattg cggcggatgc accggaagta tcggccatgg cactatcggg
     3661 agaagatcga gaaaattcgg gaagcgatgg cgacggcggc gatcggagcc gatgtcatgg
     3721 tcggatttcc gggcgagacc gaggcggagt tcgaggctac gcggcggatg gtggaggagt
     3781 tgccgtttac ttacctgcat gttttcacct attcggcgcg gccgggcaca ccggcagcgg
     3841 ctatgcggaa tcaagtt
//
LOCUS       DASPUL010000449         4564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1179373, whole genome shotgun sequence.
ACCESSION   DASPUL010000449 DASPUL010000000
VERSION     DASPUL010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179373"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VEJ00_10715"
     CDS             complement(<1..314)
                     /locus_tag="VEJ00_10715"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXX01678.1"
                     /translation="MQLDSQERRMMRRFDMRLPALVRLEGAGELATETQNVSARGVFF
                     YLDREVAAGTKLEVTLTFPPHVTLTDSVRVRFSARVIRVEHPLPSARVGTAAMIESYE
                     FLR"
     gene            complement(598..744)
                     /locus_tag="VEJ00_10720"
     CDS             complement(598..744)
                     /locus_tag="VEJ00_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01679.1"
                     /translation="MRAGARKDAGGIAQKWLQGNGRMAQGVASVMIGPRTGERKLLIL
                     AAIR"
     gene            1067..>4564
                     /locus_tag="VEJ00_10725"
     CDS             1067..>4564
                     /locus_tag="VEJ00_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HXX01680.1"
                     /translation="MQQRLETASEERVMMNRRRLGTSGLRVRVLPAGLGVLGLVLLAA
                     GFVILGGRVSHTSANLAAVQSSASGQPSPLSKLSASSPGVQATAKLRALSYFSGLPLM
                     FEPNQGQANLDPSDRRIRFLAHASGYGLYFGHDGAILTLPTQVSPAAASSAAGSVARN
                     RKPSRPRVALLEMKVAGANPDASLTAADPLPGKTNYLLGNDPAKWRRGIPQFARLRYE
                     NIYPGINLVFYGNQGHLEYDFQVSPGADPSQAELEFHGAKRLELRDGELVIQSAGSSV
                     RFNAPRVYQEIAGRQQPVEGKFVLRGDNRAGFALGPYDHSRELVIDPVLSFSTLFGGT
                     GDEHATSVAVDSSGLIYLAGSTTSPDLLTLHPGAAPGKFPDLVGAQNVYITQIDPNAP
                     QGPVINYITYLGGSGMDEPVGIAVDSGQEPMVAGTTTSPNFPVSTNAYQSSPETLGKP
                     HVFVTRFVTGAGSLLYSSYLSGNGDDIASGMTLDNSEDVYVTGTTTSTDAGTTAVQFP
                     ASALPNTPAFQTAPVAPIQFFVTVVNTGSFGPGSILYSTYFGGGATSSNAAIVATGGG
                     IAVDLNQNIYFDGTTNFVFTGSSPFDFPIKNAYQPCLDQPPPTVIINPPTCTNSGTLP
                     DAFVAKLNSPKAQQFEGGAQLLWSTYLGGTQTDSATGIAVDTGAANVYVVGTTNSQQF
                     TIPATIAPYQACLNNLPPTTGGTLPTCTVQTDPAPSDAYVARFNNPSATTANPTFNVA
                     LTYFSYLGGSTAGGGTGNDSGAAVTVDTAADAIVTGFTESKDFPIFPSPNDGIQNSLN
                     GTRDAFLARINTAAQTGQNNNSSFATYFGGSGTDEGTSITLDANQNVYFAGDTNSPDF
                     PVANNLGIQQKNNGGFDAFVTKFGTAATLGLVGQLQLGTNQTFISAGNPATFIYTLTN
                     NGPDPANNITVTDNVNASITGVSVTPTTAGATPNGTCSGVGASSTVVTCTIPQLQSGS
                     QATITITMTPAATASGNSAAFNGGVVTASAANGIGSNQVTVTAQMSDFTINVNPNSFT
                     VPEAGDTATYQVELTPHPVFGNSISLNATGAPTGARPVFSQNSVSLANSSSGGAILSI
                     STTARPIVTPGASLWKRHFYAVWLAIPGLAVLGLGTQDRRRRRLMGIIALCALSFLLL
                     LLPACSHQTTQIPPSGTPPG"
BASE COUNT          887 a         1608 c         1216 g          853 t
ORIGIN      
        1 cgaaggaatt cgtagctctc gatcatggcc gcagtgccga cccgcgcgga aggaagagga
       61 tgctcgaccc ggatcacgcg cgcgctgaac cgcacccgca ccgaatcggt cagagtgaca
      121 tgcggaggaa acgtgagcgt cacttccagc ttggtgcctg ccgcgacctc ccggtccagg
      181 tagaaaaaaa cgccgcgcgc gctcacgttc tgggtttcgg tcgcaagttc gcccgcgcct
      241 tccagacgga cgagtgcggg caggcgcatg tcaaagcgcc gcatcatccg ccgttcctgc
      301 gaatccagtt gcatgtgacg cgcgctcccg cgaaggagcg actaaccttc cgccaaagct
      361 gcaccgcgaa cccgccgctc gcctggtccg ccaggaactc gagacaatca cggttccgaa
      421 gcctcaatgt attagatgct caaccatttg caacaacaaa aacgctgccc cggaactgac
      481 tcgtcaacca tcccggagcc tcggcctacg cgcgaagaca ctggggcgcg caagtgccac
      541 ctacagggac atcgataacc caagcaatct gcctgccatc tgctctctcc ttgctcgtca
      601 ccgaattgcc gccagaatca gcagcttccg ctcgccagtg cgcggtccga tcatgacgga
      661 tgcaacccct tgcgccatcc gtccattccc ctgcagccac ttttgtgcta tgcctccggc
      721 atctttccgc gcacccgctc gcatctgacg attcttggag cgagccgccg gggatcgacc
      781 cctgcggttc gtctcccggg gaccccggac ttctcagcca gcacgggagg cccgcttgac
      841 ttgtctatcc gccggatgtt taagtaatta tttatttttt ccgccagttc cagtgcccca
      901 gcaagccgcg ggccggcgcg cccaccgcag cacgccgttg ccgagcccgg ggagtgactc
      961 gtgcgctgtc tccagtctct ccactgatct gagcgaaaac gatctgcgcg agacgatctg
     1021 agcgaaacgc ccgagcggca gcatgcgctg agggaaagaa cggcctgtgc aacaacgact
     1081 tgagaccgca tcggaggaac gcgtaatgat gaatcgccgt aggttgggaa cttccgggtt
     1141 acgagttcgc gttctccccg caggcctggg agtgctgggg ctggtattgc tggccgccgg
     1201 attcgtcatt ctcggtggac gggtaagcca cacctcggcc aacctcgctg ccgtgcagtc
     1261 gtcagcctcg ggtcagccca gtcctctgtc caagctgtcg gcctcctcgc ccggcgtgca
     1321 ggccacggca aaactccgcg cgctctcata cttctccggt ctgcccttga tgttcgagcc
     1381 gaatcagggc caagccaatc tggatccctc cgaccgtcgc atccgcttcc tggcacatgc
     1441 ctcgggctac ggtctctact tcggtcatga tggcgcaatc ctcacgttgc cgacccaggt
     1501 gtcgccggca gcagcttcct ccgctgcggg ttccgtagcc cgcaaccgga agccgtcgcg
     1561 cccccgcgtc gcactgctcg aaatgaaggt ggcaggcgcg aatccggatg ccagcctgac
     1621 cgccgccgat ccgcttcccg gcaagacgaa ctaccttctc ggcaacgacc cggcgaagtg
     1681 gcgccgcggc attccccagt tcgcccgcct gcgctacgaa aatatctacc ccggcatcaa
     1741 tctcgtcttc tacgggaatc aggggcacct tgaatacgac ttccaggtct cgcccggcgc
     1801 cgatccctcg caagctgaac tcgaattcca tggcgcaaag cgcctcgaac tacgggatgg
     1861 agagctcgtc atccagagcg ccggcagcag tgttcgcttc aacgctccgc gcgtctatca
     1921 ggagatcgcc ggccgccagc agccagttga aggcaagttc gtccttcgcg gagacaatcg
     1981 cgccggattc gccctcgggc cctacgacca ctcccgtgag ctggtcatcg atcccgtgct
     2041 cagcttttcg acgcttttcg gcggcactgg cgacgagcac gcaacatcgg tcgcggtgga
     2101 ctccagcggc ttgatctatc tcgcgggttc gaccacttcg cccgatctgc ttaccttgca
     2161 tcccggagcc gcgcctggca agtttccgga tctggtcggc gcccagaacg tctacatcac
     2221 gcaaattgat cccaacgccc cccagggacc ggtcatcaac tacatcacct acctgggcgg
     2281 cagtggcatg gatgagccgg tcggaatcgc cgtcgactcg ggccaggaac ccatggtggc
     2341 cggaaccacg acttctccca atttccctgt ttccacgaat gcctaccagt cttcacccga
     2401 aacccttggc aagccccacg tattcgtcac ccgcttcgtc actggggcgg gttcgctgct
     2461 gtattcgtcc tatctatcag gcaacggcga cgacatcgcc agcggcatga ctctcgacaa
     2521 cagcgaagac gtctatgtga ccggaaccac cacgtccaca gacgcgggca ccaccgccgt
     2581 gcagtttcct gcgagtgcgc tgccgaatac accggcattt cagaccgctc cggtggcgcc
     2641 cattcagttc ttcgtcacgg tagtgaacac gggcagcttc ggtccgggca gcattctcta
     2701 ctcaacctac tttggaggcg gagccaccag ctccaacgcc gccattgttg ccaccggcgg
     2761 aggcatcgcg gtcgacctca accagaacat ctatttcgac ggtacaacca actttgtctt
     2821 cacggggagc tcgcccttcg acttccccat caagaacgcc tatcagccct gtctggacca
     2881 acctccgcca acagtcatca tcaatccgcc gacctgcaca aatagcggta ctctgcccga
     2941 cgctttcgtg gcgaaactga attctcccaa ggcccagcaa ttcgaaggcg gcgcccaact
     3001 gctctggtcc acctatctgg gcggcacgca gactgactcg gcgaccggca tcgcagtcga
     3061 cactggcgcg gccaacgtct atgtcgtcgg gaccaccaac tcccagcaat tcacgattcc
     3121 cgcgaccatt gcgccctacc aggcttgcct gaacaatctc ccgccaacca ccggcggcac
     3181 actccccacg tgcaccgtgc agaccgatcc cgcgcccagc gacgcttacg tggcccgctt
     3241 caacaatccg tccgcgacca cggccaatcc cacgttcaat gtggcgctga cctacttctc
     3301 gtatttgggc ggaagcaccg cgggaggcgg caccggcaat gacagcggtg cggcggttac
     3361 ggtcgacacc gccgccgatg cgatcgtcac cggattcact gagtccaagg atttccccat
     3421 ctttccgtcg ccgaacgacg gcattcagaa cagcctgaac ggcacgcggg atgccttcct
     3481 ggctcgcatc aatacggccg cgcaaaccgg gcagaacaac aactcttcct ttgccaccta
     3541 ctttggcggt agtggcacgg atgaaggtac cagcatcacg ctcgatgcca accagaacgt
     3601 ctacttcgcc ggcgatacca actcccccga cttcccggtg gccaacaatc tcggcatcca
     3661 gcagaagaac aacggcggct tcgatgcgtt tgtcaccaaa ttcggcacgg ccgcgacgct
     3721 cggtcttgtc ggacagctgc agttgggcac caaccagacc ttcatcagcg caggcaatcc
     3781 cgcgaccttc atttatacgc tgactaacaa cggtccggat ccggccaaca acatcacggt
     3841 gaccgacaac gtgaacgctt cgatcaccgg agtttcagtc acccctacaa ctgccggcgc
     3901 gacccccaac ggcacctgca gtggcgttgg cgcgtcttcc actgtggtga cttgtaccat
     3961 tcctcagttg caatcaggat cccaggccac gatcaccata accatgacgc ctgccgcaac
     4021 agcctccgga aactccgcgg ccttcaacgg aggcgtggtg acggcctcgg cggccaacgg
     4081 aatcggctcc aaccaggtca ctgtcacggc gcagatgtcg gatttcacca tcaacgtcaa
     4141 tcccaacagc ttcaccgtgc ccgaagccgg cgatacggcc acgtaccagg ttgagcttac
     4201 cccgcacccg gtcttcggca attccatctc gctcaacgcc acgggcgctc ctaccggggc
     4261 ccgcccagtc ttctcccaaa actcagtgtc gttggccaat tcgagctcag gcggtgcaat
     4321 cctgagcatc agcacgactg cccgaccgat cgttaccccg ggtgcaagcc tgtggaaacg
     4381 gcatttctat gccgtgtggc tggcaattcc tggactggca gtgctgggtc tgggcactca
     4441 agaccgtcgc cggcgccgtc tgatggggat cattgcgctc tgcgctttgt ctttcctgct
     4501 tctgctgctg cctgcctgca gccaccaaac gacccagata ccgccatcgg gaacgcctcc
     4561 cggt
//
LOCUS       DASPUL010000450         4395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_917495, whole genome shotgun sequence.
ACCESSION   DASPUL010000450 DASPUL010000000
VERSION     DASPUL010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917495"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VEJ00_10730"
     CDS             complement(<1..767)
                     /locus_tag="VEJ00_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXX01681.1"
                     /translation="MIRALIDFALRNRLMILGGALVLFVWGIISFHALPIEAYPDVAD
                     TYAQVITQWPGHAAEEVEQQITVPLEVELNGVAHLTHLRSVSLMGLSVITMIYDDDIT
                     TFNARQEVLDRLQMVTLPPGVNPGIGPDYSPTGQIMFYTLTSTNPKYDVMELKSLNDW
                     FVVNQLKSVPNIVDVNPFGGPTREYQVQVDPGKLVSYGLSLAQVENALIANNINAGGG
                     FVERGEQALNVRAVGLMQTTDDIAATVVKVQNGTPVRV"
     gene            complement(764..1870)
                     /locus_tag="VEJ00_10735"
     CDS             complement(764..1870)
                     /locus_tag="VEJ00_10735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXX01682.1"
                     /translation="MLALMLAGLSVGGACSKDTPAATSKPPLTLDPDVFDVDHPELFK
                     TAKAEVRSLPAEITANGTVSPDINRTIHVTSLGSGRVADLKVKLGDTVSKGQTLLVIS
                     SPDLALAFSDLQKARADEVLSHKALDRAQMLYDRGAIAARDLEIAQDSEDKAKVDVET
                     ATQHVRVLGADPDHPSPLIELKAPVSGTIVEQNVAGFEGVKSLDNTPNLFTIANLAEV
                     WVVCDVFENDLGEVHLGDGAEIHLNAFPDRWFHGSVADISRVLDPNTRSAKVRIVLAN
                     GDGSLRPGMFAVARFRSRRTTQRVVVPATAIVRLHDEDWIFRKEGDKRFRKIAVQADG
                     LAPDGSQEIREGLKPGEEVVTNALEFSTEVAEKR"
     gene            complement(1921..3240)
                     /locus_tag="VEJ00_10740"
     CDS             complement(1921..3240)
                     /locus_tag="VEJ00_10740"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXX01683.1"
                     /translation="MRRRIWRASAFLLAVGIAAGIAPHAAAQTAAQTVAPAPPADLWT
                     WDQVKGKFEQNNATLMAGRLNIDELKAEEITSFLRPNPQFSLAVDGTQVAPSHGEWRP
                     FAGTFETPGISQLFERQHKRHLRQESARQGTAIGSAEQDDLERNLLFNLRNAFVGVLQ
                     AKAVLKVAQDNLDYYDHVLKISRDRFQAGDIAQIDLDRLELQRVQYESDLQTALVSLR
                     TTKINLLALLNDHSPVDSFDAEGPFEFSESVLALDDYRKDALDARPDLKAAVLSVQQA
                     ETNYKLAEANGSTDPTLSAWYTHNASDNNPLATNTIGASVSIPLRVFDRNQGEKLRTK
                     IDIKRNEKIRQGVETQVYSDVDSAYALVNSNIALLKPYKSKYLEQAVRVRDTVFFSYQ
                     HGGASLLDFLNAESDYRMVQLNYVNLVGAYLTSAAQINLAVGREVIP"
BASE COUNT          820 a         1322 c         1354 g          899 t
ORIGIN      
        1 actcttaccg gcgtgccgtt ctgcaccttg accacggtcg ccgcaatgtc gtcggtggtc
       61 tgcatcaggc cgacggcgcg cacgttgagt gcttgttcgc cgcgctcgac gaatccgccg
      121 ccggcgttga tattgttggc aatcagggcg ttttcgacct gggccaacga gagaccgtac
      181 gaaaccagct ttcccggatc gacctgcacc tgatattcgc gtgtggggcc gccgaagggg
      241 tttacgtcca caatattggg taccgacttc aactgattta cgacgaacca gtcattcagc
      301 gacttcagtt ccatcacgtc gtacttggga ttggtgctgg tgagcgtgta gaacatgatc
      361 tgcccggtcg gactgtaatc cggaccgata ccgggattca ctccgggcgg cagcgtcacc
      421 atctgcagac ggtcgagaac ttcctggcga gcgttaaagg ttgtgatgtc atcgtcataa
      481 atcatagtga tgacggacaa acccatcagc gataccgagc gtaagtgtgt gagatgcgcg
      541 acgccgttca gttcgacttc aagcgggacg gtaatctgct gttcgacttc ttcggcggcg
      601 tggcccggcc actgcgtgat gacctgggcg taggtatcgg ccacatcggg atacgcttcg
      661 atgggtaagg cgtggaacga gatgatgccc cagacgaaca gcacgagtgc gccgcccagg
      721 atcatcaggc ggttgcgcag cgcgaagtcg atcagcgccc ggatcatcgc ttctccgcca
      781 cctcggtgga gaattccagc gcattggtca cgacttcctc gccgggcttc aggccctcgc
      841 ggatctcctg cgagccgtcc ggagccaggc cgtcggcttg cacggcaatc ttgcggaagc
      901 gcttgtcgcc ttccttgcgg aaaatccaat cctcgtcgtg caggcgaaca atcgcagtcg
      961 caggcactac cacccgctgg gtggtcctgc gcgagcgaaa ccttgctacg gcgaacatgc
     1021 caggccgcaa cgagccgtca ccgttggcca gcacgatgcg gactttggcc gagcgcgtgt
     1081 tgggatcgag cactctggaa atgtcggcga cgctgccgtg aaaccaccgg tcgggaaagg
     1141 cattcaggtg gatttcggcc ccatctccca gatgcacttc cccaagatcg ttctcgaaca
     1201 cgtcgcacac cacccagact tcggcgaggt tggcgatggt gaacagattg ggagtgttgt
     1261 ccaggctctt aacaccttca aatcccgcca cgttctgttc gacgatcgtt ccgctgaccg
     1321 gcgctttcag ttcgatcaac ggactgggat ggtcaggatc ggcgcccaga acccgcacgt
     1381 gctgggtggc cgtttccacg tcgactttgg ctttgtcttc cgagtcttgc gcgatctcca
     1441 agtccctggc tgcgattgct cctcggtcat ataacatctg cgcacgatcg agcgctttat
     1501 gcgagagcac ttcatccgct ctggctttct gcagatcgga gaacgccaaa gccaggtcag
     1561 gactcgaaat gaccagcagc gtctgaccct tgctcacggt gtcgcccagc ttcaccttca
     1621 agtcggcgac ccggccggag ccgagcgagg tgacatggat cgtccggttt atgtccgggg
     1681 agacggttcc gtttgccgta atttcggcgg gcaacgaccg cacctcagcc ttggccgtct
     1741 tgaacaactc ggggtggtcg acgtcgaaga cgtcgggatc aagcgtgagg ggcggcttgc
     1801 tggtcgccgc cggagtgtct ttggagcagg ccccgccgac agaaagaccg gcgagcatca
     1861 aagccagcac tgaccaacgc cgccagcaaa ggccattgcc aggcctgcga aacaggtttg
     1921 tcatggaatg acctctcggc ctaccgccag attgatctgc gcggccgacg tcagatatgc
     1981 gcccaccaga tttacgtagt tcagctgcac catgcggtag tcactttcag cgttcaggaa
     2041 gtccagcagc gacgctccgc cgtgctggta ggaaaagaaa accgtgtcgc gcacccgcac
     2101 tgcctgctcg agatacttgc tcttgtaggg cttcagcaag gcaatgttgc tgttcacgag
     2161 ggcgtaggcg gagtccacgt cgctgtagac ctgggtctcg actccctggc ggatcttctc
     2221 atttcgcttg atgtcaattt tcgtgcgcag cttctcgccc tggttgcggt caaagacccg
     2281 gagcggaatg ctcacgctgg ccccaatcgt gttcgtggcc agcggattgt tgtcggaggc
     2341 gttgtgggta taccacgcgc tcaaggtcgg atccgtggaa ccgttggctt cggccaattt
     2401 gtagttggtc tccgcttgct gtacggagag caccgcggcc ttgagatcgg ggcgggcgtc
     2461 gagggcgtcc ttgcgatagt cgtcgagtgc cagcacgctc tcgctgaact cgaaggggcc
     2521 ctcggcgtcg aagctgtcca ccgggctgtg gtcattgagc agcgccagga gattgatttt
     2581 ggtggtcctc aggctgacca gcgccgtctg gaggtcagat tcgtactgca cccgctgcaa
     2641 ctccagccgg tcgagatcga tctgcgcgat gtcgccggcc tggaagcggt cccggctgat
     2701 tttgagcacg tgatcgtagt agtcgagatt gtcttgcgct accttcagca ccgctttggc
     2761 ctgcagcaca ccaacaaacg cattgcgcag gttgaacaga agattgcgct ccaggtcgtc
     2821 ttgctcggcg ctgccgatcg cggtgccctg cctggcgctt tcctgccgca ggtgacgctt
     2881 gtgctgccgc tcgaacaact ggctgatgcc cggggtttca aatgtgcccg caaaaggtcg
     2941 ccactctccg tgactgggag cgacttgcgt cccatctacc gccagcgaaa actgcgggtt
     3001 tggccgcaag aaggaggtga tctcctccgc cttgagttca tcgatgttca accgccccgc
     3061 catcagggtc gcgttgttct gctcgaactt gcctttgacc tgatcccagg tccataggtc
     3121 cgctggcggc gccggcgcaa ccgtctgggc ggcagtctgc gcggcggcgt ggggcgcaat
     3181 acccgccgcg attccaaccg ccagtagaaa tgcggacgct cgccagattc ttctgcgcat
     3241 tcggaactcc aaaagtctcc actcagccgc ggtcgaaggg cgccttcgac gcggatgcct
     3301 tcacatcatc cgattgacct ttaatatatg gtctcccccg caggaagtgc gtgacgaccg
     3361 gggggaagct tcgccgcagt cttcacaact tgccggcgaa ttggcgacta cactgggagg
     3421 tcttggggag gggcgcggct cgtgggagcg gcagcgggcg cggctggcat acgcaacgcc
     3481 gggcgccaca ggggctgtct gtcttcgcca ttcgctggag ccagatggat cggtgccacc
     3541 agcgcggctg cgtgctgtcc tctcgcgttc cagatcgggc ccccaccgcc tcgtggagac
     3601 tgcgcttact gatgggcgat tcctccattt cagtccgcat cgcgagcaaa tcgtcggttg
     3661 cggaaacgac cgagaacaga ataaccagca cgcacgccag ggtcagcaga cacggcaagg
     3721 atccgcaggc tcgcgtggcc ccgccccgac gccagagcca ataggccggc accagcaaca
     3781 gaagccaggc tagattgagc agcagttcca taacggggca gcgccccaac ccactccgag
     3841 agggtagttt ttcagataga tcgaatctga gtcaatgtga cttccgtgac gcggtgggcg
     3901 agctgcgacc gggcgtcatc ccgcgcgaag ccgtttttgg tagacctccg gccttcagcc
     3961 ggaggcgagc gcagcgaggt ggagcgaggg atctcccgat atagggggcc gttgcgtggg
     4021 aggtctcttg tcccgccggt gaaaacgcga cacttcgaga ggacgccggt acaagctgcg
     4081 atttcaaact gaggcacgac tgcaggattt cgccgtctcc tcgttctatg tgatgataac
     4141 cacagggttg cagtgcgcag cgggaaacgg cagtccctgg atggccggca tgaggaaatt
     4201 gcgttgcatg cggaacacaa tgagcaggct gctggtgctt gcctgcggtg tttggccagt
     4261 gtgttgcctg gtaccaccgg ccgcagcaca gagcggtgcg ccgacagcgc agtccagtca
     4321 gaccttgatg atcgagaacg cgtccccgct agccgaaacc tatcctggcg cgagatacga
     4381 agtttatttc cgcgc
//
LOCUS       DASPUL010000451         6130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1729167, whole genome shotgun sequence.
ACCESSION   DASPUL010000451 DASPUL010000000
VERSION     DASPUL010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729167"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VEJ00_10745"
     CDS             complement(<1..541)
                     /locus_tag="VEJ00_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HXX01684.1"
                     /translation="MMKTYRLIHAKAARTKIRVPMVWSWHVGLKRQDVFMASYPRSGS
                     TWLRFMLFEILSGEEPGFRKLEDRLPEIQHQRGKAPILPNGGRLIKTHEQYRSDYKRA
                     IFLVRDVRDVLLSGYARAVEVGIADLVSKGDLDSFLMAFLQGSALQMGSWQEHSRSYL
                     DSPLAKNGNLLVVKYEELRK"
     gene            complement(607..1980)
                     /locus_tag="VEJ00_10750"
     CDS             complement(607..1980)
                     /locus_tag="VEJ00_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01685.1"
                     /translation="MTETANSTRRFSLGSLLATDGASVRRKVIPWAMKGGLALLDQGV
                     FAGSNFVMSILLARWLSPEQYGAYAVAFAVFLFLLNFHQALLLEPMLVFGSSVYRSSL
                     RGYMKALLVAHSAMSLLMFVGLCVAAAVTVKASPANGLPGALFGIAVAAPAVLLFWLT
                     KRSFYLKLSPAPSAAAAVLYCILTMGGMALVYKYTHTLSPLSAFLLMAFGGLGASLVL
                     LVYLNAQLPRDHEAPRLAETWGRHWRYGRWALGANAMMWIPINAFYPILSKFSGMAQA
                     GELKALMNFAAPMLQACAALHSLMLPYASRVLAQRGGDGISVILRRMTFLCVACAVPY
                     WVLLLLFRGPAFHLLYSGRYTEVAYLLPVVALASVAGSAFFGPSIVLRSLESPGLVFA
                     AVTVSSVIAVAVGIPLTWALGLSGAVWSIALSETLAFVTAVVMVRRKGRKTCEAATPL
                     FALSANE"
     gene            complement(2010..5384)
                     /locus_tag="VEJ00_10755"
     CDS             complement(2010..5384)
                     /locus_tag="VEJ00_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HXX01686.1"
                     /translation="MNGSEFRPGADVERAIEYWRDRLAGIPAILELPTDRPRPPAQSF
                     RAARESAILPESLVRTLEAWGEREGASLFALLLAAFQTLLTRYTRQDDIVVGSILPGQ
                     EEGTTDGVIGPFDIPLPIRTDMSGDPTFRELLAGVNDALAGAREHRNIPWALLVEAVQ
                     AEQDASRHPVYQAAFSFEDARFLPEQDWSATDSAADTLKVDLQLQLCRQSNGMVVHFT
                     YSTDLFDAGTIVRMAGHFQQLLQGIAANPYEKISRLPLLTEAERHQLVVEWNDTAMEY
                     PKDRCVHQLFEAQAAKTPDAIAVMFEGQRLTYRELDQRANQLARHLISLGARPDGLIG
                     ICLERSLEMIVGVIGILKAGSAYVPLDPAYPRDRIAFMLENSEVPLLLTQTQLADTLP
                     KCNAKVVLIDAHWPEIARQSPESPALSLDPENRAYVIYTSGSTGKPKGVEIPHRAVVN
                     LLTSMIDQPGFSSSDRLLAVTTLSFDIAGIEIHLPLTRGASFEIVSREVAADGNRLLA
                     KLGSSGATVLQATPATFRMLLEAGWAGDPQLKIIIGGEAVSHKLASQLLERAGSVWNQ
                     YGPTETTIWSTGSKLEVGQGVTIGRPFGNTETFILDTVLQPVPIGVAGELHIGGDGLA
                     RGYLKRPELTAEKFIAHPLSADPKARLYKTGDLVRYLPNGKIEFLGRIDHQVKIRGFR
                     IELGEIEAVLRQHPRINDTVVVAREDVPGDKRVVAYVVPAGEPALTTAELRTALKEKL
                     PEYMVPSIFVTLKSLPLTPNGKVDRRALPAPSEADLAPMGMFAAPRDAVESRLAQIWE
                     TVLGVRPIGVTDTYFDLGGHSILAVKLMHHIEEAFGKNLPIATLLQAPTIEQLAAILR
                     QKEALPAWSCLVPIQTAGSRPPFFCIHGANGAVVRFHALSRYLGADQPFYGLQAQGLG
                     AGQSCHGRTEEMAAHYIKEVRKVQLKGPYFLGGYSFGGAIAFEMAQQLSAQGHGESVV
                     VLFDTNFPQSAFASVSASAALLEFFRAPVSKKPGYLWRILTVPLREVERRLQVARLPE
                     NSKKVRKACLQAERDYRPQAYSGRVILYRSNHKPLGQLADPRAGWSNCAARGLEIREV
                     AGNHENILLEPQVRSVAESLKACLDETLVSQQRTGR"
     gene            complement(5514..>6130)
                     /locus_tag="VEJ00_10760"
     CDS             complement(5514..>6130)
                     /locus_tag="VEJ00_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01687.1"
                     /translation="ELFGKFIWSRRATEVFARETSRWDGDALIGALDTSDRDVSEAQF
                     GDLVDMAWEHPEIFTLSELPQCRQRMKNGDRLYVVREKGSVVLLCWVSGWSGAAADSA
                     TPRMEATPGTPAVLVDEYWRARDRDLSLSYRLLLSILTREAACRKSDLLVHCASNQPA
                     LRIELERQSFVPRFHTTRRSILGRQREFISRYPENSTLRPSQAA"
BASE COUNT         1268 a         1940 c         1797 g         1125 t
ORIGIN      
        1 tcttgcgcag ctcctcgtac ttcacgacca ggagattgcc gttcttggcc agcggagaat
       61 ccaggtacga gcgcgagtgc tcttgccagg agcccatctg gagagcgcta ccctgaagga
      121 acgccataag gaagctgtcg agatcgccct tcgagaccag atcggcgata cccacctcca
      181 ccgctcgggc atatccggag agcagaacat ctctgacatc gcgcaccagg aaaatggctc
      241 gcttgtagtc actgcgatat tgttcgtggg tcttgatcag gcggccgcca ttgggcaaaa
      301 tcggcgcctt gccgcgctga tgctggatct caggcaggcg atcttcgagt ttgcgaaacc
      361 ccggctcttc cccggacagg atctcgaaca gcatgaagcg cagccaggtg ctacccgaac
      421 gaggatatga ggccataaag acgtcctggc gcttgaggcc aacgtgccag gaccacacca
      481 tcgggacgcg aatcttcgtt cttgctgcct tcgcgtgaat cagtcggtaa gtcttcatca
      541 tagttgtgtt tccctcactg tgaattctga agagatgatt ggatcggatc ttgatggggg
      601 atccagctac tcattggccg agagtgcgaa cagaggcgtg gcagcctcgc aggtctttcg
      661 gcctttgcgg cggaccatga caacggccgt cacgaaagcg agcgtttccg acagcgcgat
      721 gctccacacg gctccgctca agcccagggc ccaggtgagc ggaatgccga ctgctaccgc
      781 aatcacgctg gaaaccgtaa ccgcggcgaa caccaacccc ggcgactcca gggagcgcaa
      841 cacgattgac ggcccgaaga aagcgctgcc cgccaccgat gccagcgcaa cgactggcaa
      901 caggtaagcc acttccgtgt accggccgga ataaagaaga tgaaacgccg gtccgcggaa
      961 caagagcagc aatacccagt acggcacagc gcaggcaaca cacaggaatg tcattctccg
     1021 caggatgacg ctgattccgt cgccgcccct ctgcgccagt acgcgcgagg cgtaaggcag
     1081 catcagggag tggagcgcgg cgcaagcttg taacatcggg gccgcgaagt tcatcagcgc
     1141 cttcagttcg cccgcctgag ccatgcccga gaacttgctc agaatcgggt agaaggcgtt
     1201 gatcggaatc cacatcatgg cgttggcgcc gagcgcccat ctgccatatc gccaatgccg
     1261 tccccaggtt tctgccaggc gaggcgcctc gtggtcccgt ggcaattgcg cattcaagta
     1321 caccagcagt accaggctcg ctcccaagcc gccgaatgcc atcagcagga acgcggagag
     1381 cggcgaaagt gtatgggtgt acttgtaaac gagggccatg ccccccatgg tcagaatgca
     1441 gtagagcacg gcggccgccg ccgaaggtgc cggcgacagc ttcaggtaga aggatcgctt
     1501 ggtcagccag aacagcagaa ccgccggcgc cgcgaccgcg atcccaaaca gggctccggg
     1561 cagtccgttg gctgggctcg ccttaaccgt gactgcggcc gccacgcaca atcccacgaa
     1621 catcagcaga ctcatggccg aatgagccac cagcagggcc ttcatgtaac cgcgcagcga
     1681 actgcgatag accgagctgc cgaacacaag catgggctcc agcagcagcg cctggtggaa
     1741 attcagaagg aacagaaaaa ccgcgaacgc cacagcgtag gcgccatatt gttctggaga
     1801 cagccaccgg gccagcagga tgctcatgac aaagttcgat ccggcaaaga ctccctggtc
     1861 gagcagcgcc aacccgccct tcatcgccca ggggatgacc ttcctgcgca cgctggctcc
     1921 atcggtcgcc agaaggcttc cgagcgagaa ccgacgggtg gagttcgccg tctcggtcac
     1981 gatcgatgct tcctgaactc tcaagctgct cacctgcccg tcctttgctg ggaaacgaga
     2041 gtctcgtcga gacaggcttt caaagactcg gcgaccgacc gcacttgcgg ctcgagcaga
     2101 atgttctcgt gattgccggc aacttcacgg atctccaggc cgcgggcggc acaattgctc
     2161 catccggcac gcgggtccgc caactggccg agcggcttgt ggttggagcg gtacaaaatg
     2221 acccgtcctg agtatgcttg cggccggtag tctcgctccg cctgaaggca cgcttttcgg
     2281 accttcttgc tgttctccgg caaccgagca acctgcagcc gtcgttctac ttccctcagg
     2341 ggtacagtaa gaattcgcca caagtacccc ggctttttcg acaccggagc ccggaaaaat
     2401 tcgagcagcg ccgccgaagc ggacaccgaa gcaaacgcgc tctgagggaa gttggtgtca
     2461 aacagcacca ccacggattc cccatgcccc tgggctgaca gctgctgggc catctcgaag
     2521 gcgatggcgc cgccgaagga atagcctccc agaaaataag gtcctttgag ctgaaccttc
     2581 cgcacttcct tgatgtagtg cgcggccatc tcttcggtgc gcccgtggca gctttgtccc
     2641 gcgcccaggc cctgcgcctg cagaccgtag aacggttgat cggctcccag gtatcgcgat
     2701 aaggcgtgaa aacggacgac cgcaccgttc gctccgtgga tacagaagaa cggcggcctc
     2761 gagcccgcgg tctgaatcgg taccaggcac gaccacgccg gcagcgcttc tttctggcgc
     2821 agaatcgccg ccagttgctc gatggtcggg gcctgcagca gagttgcgat cggcaggttc
     2881 tttccaaagg cctcttcgat gtggtgcatc agcttgactg ccagaatgga atgcccgccg
     2941 aggtcgaagt aagtgtccgt gacaccaatg ggccgcacac ccaaaaccgt ttcccaaatc
     3001 tgcgccagac gagactcgac agcatccctc ggtgcagcaa acattcccat gggcgcaaga
     3061 tcggcctcac tgggagcggg cagcgcgcgc cggtccacct tgccgttcgg cgtgagcggc
     3121 aggctcttca gagttacgaa aatcgaaggc accatgtact ccgggagctt ttccttcagc
     3181 gccgtacgga gttcggcggt ggtcagcgcc ggctcgccgg ccggaacaac atatgcgaca
     3241 actcgcttgt cgccagggac atcttcgcgc gctaccacta ccgtgtcgtt gatccgcgga
     3301 tgctgccgca gcacggcctc gatctcaccc agttcgatgc ggaagccgcg aatcttcacc
     3361 tggtggtcga tccgccccag aaactcgatc ttgccgttgg gcagatagcg tacgagatcc
     3421 ccagttttgt acaggcgcgc tttcggatcg gcgctcaacg gatgcgcaat gaatttttcg
     3481 gccgtgagtt cgggccgctt gagatagccg cgagccagcc cgtccccgcc gatgtgcagc
     3541 tctccggcaa ccccgatcgg caccggctgc agcaccgtgt cgaggatgaa agtctcagtg
     3601 ttgccaaaag gccgtccgat cgtgacgccc tgacccactt ccagcttcga gcccgtcgac
     3661 cagatcgtgg tctcggtcgg accgtactgg ttccacaccg atccggcgcg ctccagaagc
     3721 tggctggcca gcttgtggga gacagcttca ccgccaatga tgatctttag ctgcggatct
     3781 ccggcccatc ccgcctccag aagcatgcga aaggtggccg gagtggcctg caggaccgtc
     3841 gcacccgaac tgcccagctt ggccagcagt cgattgccgt cggccgcgac ttcgcggctg
     3901 acaatctcga aggatgcacc ccgtgtgagc ggcagatgga tctcaatacc tgcaatatcg
     3961 aacgagagcg tggtgacggc cagcagacgg tcgcttgaac taaaccccgg ctggtcgatc
     4021 attgaggtca ggagattcac gaccgcgcgg tgcgggatct ccactccctt gggcttcccc
     4081 gtggatccgg aggtatagat cacgtaggcg cgattctcag gatcgaggct gagcgcagga
     4141 ctctctgggc tttgccgcgc aatttccggc cagtgtgcgt cgatgaggac gaccttggca
     4201 ttacacttcg gcaaagtgtc cgccagctgc gtttgcgtca gcagcaacgg cacttcggaa
     4261 ttttccagca tgaacgcgat gcggtcgcgt ggataggccg gatcaagcgg cacatacgcc
     4321 gacccagcct tgagaattcc aatcacgccg acgatcattt cgagcgagcg ttccaggcag
     4381 attccaatca gcccgtccgg gcgcgctcca aggctgatca ggtgacgagc cagctgattc
     4441 gcgcgttggt cgagctcgcg gtaggtcagg cgctggccct cgaacatcac cgctattgca
     4501 tccggagtct tcgccgcttg tgcttcgaaa agctgatgaa cgcaacggtc tttcgggtat
     4561 tccatcgccg tgtcgttcca ctccacgacc agctgatgcc gttccgcttc ggtcagcagc
     4621 ggcagacgcg agatcttttc gtacggattc gctgcgattc cttgcagcaa ttgctggaaa
     4681 tgtcccgcca tgcgcacgat cgtgcctgca tcgaacaggt ccgtgctgta agtgaagtgc
     4741 acgaccatcc cgttggactg ccggcagagc tgaagctgca aatcgacctt cagcgtatcg
     4801 gcagctgaat ccgtcgctga ccaatcctgt tccggcagga acctcgcatc ctcgaatgag
     4861 aacgcggcct ggtatacagg atggcggctg gcgtcctgtt ccgcctggac ggcctcgacc
     4921 agcagcgccc aaggaatatt ccgatgctca cgcgctccgg ctagcgcatc attcaccccc
     4981 gctaacagct cgcggaacgt gggatcgccg ctcatgtccg tgcggattgg cagcggaatg
     5041 tcgaacggtc caatcactcc gtctgtcgtg ccctcttcct ggccgggaag aatcgagccg
     5101 acaacgatgt cgtcctggcg cgtgtagcgg gtcagcagcg tctggaatgc ggccaggagc
     5161 agcgcaaaca acgaagctcc ctcgcgctcg ccccacgctt ccagcgttcg caccaaactc
     5221 tcggggagaa tcgctgactc ccgcgccgcg cggaaagact gtgccggtgg acgcggacgg
     5281 tcggtcggca attccaggat ggcaggaatc cccgccagcc ggtcgcgcca gtactcgatt
     5341 gcccgctcaa cgtcggcgcc cggacgaaac tccgaaccgt tcatcggggt ctcgcccggc
     5401 acaacctgaa gcgaaccaga tttaagattg acgtcattca taggagcgtc ggacaaagtt
     5461 tgtgacgatc gagcggccat gttttaagga attcgcagtt tcaactttcg gtcttatgcg
     5521 gcctgcgagg gccggagagt gctattttcc ggataacgtg aaatgaattc gcgctgacgg
     5581 cccaggatgc tgcgccgggt agtgtgaaac ctcgggacaa aactttgccg ctcgagttcg
     5641 atgcgcaacg cgggctggtt cgaagcgcag tgcaccagca gatccgattt gcggcaagcc
     5701 gcctcgcggg tcagaatcga caaaagcaac cggtacgaca gggaaagatc ccgatcgcgc
     5761 gccctccagt actcatcgac cagaacagcc ggagttccgg gcgtagcttc catacgcggc
     5821 gtggcggaat cggctgctgc gccactccac ccggacaccc agcagagaag caccacactg
     5881 cccttctccc ggacaacata gaggcggtcg ccgttcttca tccgctggcg gcattgcggc
     5941 agctcgctca gggtgaagat ctcaggatgt tcccaggcca tatccaccag atcgccaaat
     6001 tgcgcctcgc tcacgtcgcg gtcactggta tcgagcgcgc cgatcagcgc atccccatcc
     6061 caccgcgatg tctcgcgggc aaatacttcg gtagcccgcc tgctccaaat gaacttgcca
     6121 aatagttccg
//
LOCUS       DASPUL010000452         3776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_226037, whole genome shotgun sequence.
ACCESSION   DASPUL010000452 DASPUL010000000
VERSION     DASPUL010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226037"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VEJ00_10765"
     CDS             complement(<1..224)
                     /locus_tag="VEJ00_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01688.1"
                     /translation="MLIAGVTGWAAVATAQEDLPQINPGERKIPRKKDEGPRALALLK
                     LTADGKASLLPITILINGKFWDATAYKADPV"
     gene            346..825
                     /gene="bcp"
                     /locus_tag="VEJ00_10770"
     CDS             346..825
                     /gene="bcp"
                     /locus_tag="VEJ00_10770"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:472..474,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HXX01689.1"
                     /translation="MNINDKAPEFTLQDENGKDVALKDLRGKVVILFFYPRANTPGUT
                     VEARGFRDTYKQIQKTGAVLLGISPDTPGAQKKFAQQHSLPFTLLADTDKKVAIAFGV
                     LREKNMYGRKVLGIARTTFIIGPDGKIQHIFEKVKPEGHAEEVLAYLKESGKSQARA"
     gene            822..1454
                     /locus_tag="VEJ00_10775"
     CDS             822..1454
                     /locus_tag="VEJ00_10775"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HXX01690.1"
                     /translation="MKESQTAVRPLTRGFFARDPRRVARELLGKVLVRTTGRSRLTAR
                     IVEVEAYLGEKDPAAHAFSGRTLRNAVLFGPPGHAYVYFIYGNHYCLNISCEQEGQAG
                     GVLFRALEPLSGIDQMARARRVEIRTPSDLPKLTSGPGRLAQAFGITRARDNGCDLTS
                     SSGSLWIGADGYRRTAVRLTPRIGISKAAERKLRYLLAGNQFVSGSKTAA"
     gene            1686..2447
                     /locus_tag="VEJ00_10780"
     CDS             1686..2447
                     /locus_tag="VEJ00_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01691.1"
                     /translation="MPRLTRFRILLAAFLTLAAATPALKAAEGAPIGHAQTPPSYIVI
                     GFVGGFVHHDNPHQNSVQLARRLQNNSPPGTYIQIFENRHRSAAYKAILGLLDRDHDG
                     SLNTDEKAGARIILFGHSWGASAAVMLARELDRAGIPVLLTVQVDSVHKPWQRDEIIP
                     PNVAAAANFYQPHGLVHGRASITAADPARTEILGNFRFDYQKDPVKCEAISWFDRLTP
                     SHAQSQCDPRLWSQVENLVRQRLQPQPSTVAVNAP"
     gene            2597..3508
                     /gene="panB"
                     /locus_tag="VEJ00_10785"
     CDS             2597..3508
                     /gene="panB"
                     /locus_tag="VEJ00_10785"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXX01692.1"
                     /translation="MSLTPIGVRRFDESRPKITTASLRESKVRHEPITCLTAYDYATA
                     RLVDEAGVEMVLVGDSLAQTMLGYENTLSVTMDEMLHHVKAVRRGVKNALLVADMPYG
                     SYQIDPKTAVGNATRFVKEGGAEVVKIEGGEKRADLIRQIIDAEIPVAGHIGLTPQSV
                     NVMGGYKVQGKNLSGIEQLMRDAVALDRAGVACLYLEGIPREVAAMITAEVGAPTIGI
                     GAGPECDGQVLVFHDILNLTFGPAAKFVRRYGDAAALITSAVQAFRADVRSRHYPADE
                     ESYHLPKETKAGLDTVLARKHALRGRA"
     gene            complement(3562..>3776)
                     /locus_tag="VEJ00_10790"
     CDS             complement(3562..>3776)
                     /locus_tag="VEJ00_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01693.1"
                     /translation="RRQEIQHKAETQEVKEEQRQGMHGHTSQLNNSLAPSGGICGESA
                     AGGFSICDCRFSICNRGSNQPRMMSR"
BASE COUNT          748 a         1187 c         1095 g          746 t
ORIGIN      
        1 acggggtcgg ccttgtaggc ggtcgcgtcc cagaacttgc cgttgatcag aattgtgatc
       61 ggtagcaagg aagccttacc gtcggcggtc agtttcagca gggccagcgc ccgcggtccc
      121 tcgtccttct tccgcgggat cttgcgctct ccgggattga tttgcggaag gtcctcttgc
      181 gcagtcgcga ctgccgccca accagtgacc ccggctatca acaagaccag gcacgcggcc
      241 aggtatagaa tccggcagtg tagaatccgg tggagcggca gagggtcgga tgccgttctt
      301 gcggcatgcg ccggtctgga gttcgatttc ccctgagaga ctcccatgaa tatcaatgat
      361 aaagcgcctg aatttacttt gcaggacgag aacggaaagg acgtggcgct taaagacctg
      421 cgcggcaaag tcgtcatcct gttcttctac ccgcgggcca atactcccgg ttgaactgtc
      481 gaagctcgcg ggttccgcga cacatataaa cagattcaaa aaaccggcgc cgtgctgctt
      541 ggcatctcgc ccgatacccc gggcgcgcag aagaaatttg cgcaacagca cagtctgccc
      601 ttcaccctgc tggctgatac cgacaagaaa gttgccattg ccttcggtgt cctgcgggag
      661 aaaaacatgt acggaaggaa ggtcttgggc atcgcccgga ctacgttcat catcggcccc
      721 gatggcaaga ttcaacacat ttttgagaag gtcaagccgg agggccatgc cgaagaagtg
      781 ctggcctacc tgaaggagtc gggtaaaagc caggcccggg catgaaggaa tctcaaactg
      841 ccgtccgtcc tctgactcgc ggcttcttcg cccgtgaccc acgccgggtg gctcgcgaac
      901 tgctcggcaa ggtgctggta cggaccacgg gcagaagccg tcttaccgcc cgcattgtcg
      961 aggtggaggc ctatctcgga gaaaaagacc ctgctgccca tgccttttct ggccggacgc
     1021 tccgcaatgc cgtacttttt gggccacccg gccacgccta tgtctatttc atctatggca
     1081 atcattactg cctgaatatc tcctgcgagc aggaaggcca ggcgggaggg gttctgtttc
     1141 gagccctcga accgctttcc ggcatcgatc agatggcccg cgcccgcaga gtcgagatcc
     1201 gcactccgag cgacctgccc aaactcacca gcggccccgg acggctggcc caggctttcg
     1261 gcatcacccg cgctcgcgat aacggatgcg atctcacctc atcctcaggc agcctttgga
     1321 tcggcgcgga cgggtatcgc cgcacagccg ttcgcctgac gccacgaatc gggatatcca
     1381 aggcggccga gagaaagctg cgttacctcc ttgccggcaa ccagttcgtg tcgggatcaa
     1441 aaactgcagc gtaggatcgc ctggccgcgg gatcgctcgc ggtcggggcg gaggcggaat
     1501 ttccccgctt ggctcttgac aaacgggtgt aaactaccca acagctcgat tgagccaaaa
     1561 gccctgctgg gcacggcttt tcggccgccc tttgcgagct cccggcgtac tgctcaaacg
     1621 cccggctgaa atttttgacg catcccccag gttgctcgat tcatctacgg gattaggagc
     1681 cccgcgtgcc tcgcctcacg cgatttcgga ttctgctggc agcgtttctc accctggccg
     1741 ctgcaactcc ggcgctcaag gccgccgagg gtgccccaat cggccacgcg cagacgccgc
     1801 ccagctacat cgtgattggc tttgtgggcg gattcgtcca ccacgacaat ccgcaccaga
     1861 attccgtgca gctcgcccgt cgcctgcaga acaactcgcc accgggcacc tacattcaga
     1921 tcttcgagaa tcggcaccgc agcgccgcgt acaaggcgat tctcggcctc ctcgaccgcg
     1981 atcatgacgg cagcctgaac acggacgaaa aagcgggggc tcggatcatc ctgttcggcc
     2041 acagttgggg cgcatcggcg gcggtcatgc tggctcgcga actggatcgc gccggcattc
     2101 ccgtgctgct taccgtccag gtcgacagcg ttcataaacc ttggcagcgg gacgaaatca
     2161 tccccccgaa cgtggccgca gcggcgaatt tctaccagcc ccatggactt gttcacggaa
     2221 gagccagtat tacggctgcc gatcctgcca ggacggaaat ccttggaaac ttccgctttg
     2281 actatcagaa agatccggtg aagtgtgaag ccatctcgtg gttcgatcgc cttaccccaa
     2341 gccacgccca gagccaatgc gatccacgtc tgtggtcgca ggtcgaaaac ctggtgcgtc
     2401 agcgtctgca gccgcaaccc agcactgtgg ccgtcaacgc gccctaaggc ctgctcgatc
     2461 ccacggctct aagccctttc cagccagtaa attagctctg gtggtaaaac gtcacttttg
     2521 cgtctaaaat actcgcagca tcattcgatg tgtacaggct ctatccggcc gggccggagg
     2581 ggcactggag gacatcatga gtttgacccc gattggcgtt cgtcgtttcg acgaatcgcg
     2641 ccctaagatc accaccgctt ctctgcggga aagtaaagtc cgccacgaac cgatcacctg
     2701 cctgactgcg tacgactacg ccaccgcgcg cctggtcgac gaggccggcg tggagatggt
     2761 tctggtcggc gactccctgg cccagaccat gctgggttat gagaacacgc tctcggtcac
     2821 catggatgag atgctgcacc acgtgaaagc cgtgcggcgt ggcgtgaaga atgccctgct
     2881 ggtagccgac atgccctacg ggtcgtatca gatcgaccct aagactgccg tcggcaacgc
     2941 cacacgcttc gtgaaagaag gcggcgccga ggtagtcaag atcgagggtg gcgagaagcg
     3001 tgccgacctg atccgacaga tcatcgacgc cgagatccct gtcgctggcc acattggcct
     3061 gaccccccag tccgtcaatg tcatgggcgg gtacaaggtc cagggcaaga accttagcgg
     3121 catcgagcaa ttgatgcgtg atgccgttgc gctcgaccgc gccggcgtcg cctgtctcta
     3181 cctcgaaggc atcccgcggg aagtagcggc catgatcacg gcggaggttg gcgcgccgac
     3241 catcggcatt ggtgccgggc cggaatgtga cggccaggtg ctcgttttcc acgacatcct
     3301 gaacctgacc tttggtcctg cggcgaagtt cgtgcggcgt tacggcgacg cggcggccct
     3361 tatcaccagc gcagtgcagg cctttcgcgc cgacgtgcgc tcgcgccact atccggcgga
     3421 tgaggagtcc tatcacctgc ccaaagagac caaagcgggc ctcgacactg tgctcgcgcg
     3481 aaagcatgcc ctgcgcggaa gggcctgatc aacgtgaaga gggtgacgcc aattgccgat
     3541 ctgcgcttgc cgattctcaa ctcagcgact catcatccgc ggctgatttg aaccacgatt
     3601 acaaatcgaa aatcggcaat cgcaaatcga gaatcccccg gcggcgctct cgccgcaaat
     3661 cccgccggat ggtgctaggc tattgtttag ctgggaggta tgaccatgca tcccttggcg
     3721 ttgttcttct ttcacttcct gggtttcggc tttgtgctgt atttcctgcc ttcgat
//
LOCUS       DASPUL010000453         2940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1037518, whole genome shotgun sequence.
ACCESSION   DASPUL010000453 DASPUL010000000
VERSION     DASPUL010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037518"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VEJ00_10795"
     CDS             <1..265
                     /locus_tag="VEJ00_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01694.1"
                     /translation="RFLPQIASWRVRRLIYRWYGQLIVLEHDVQAREGDLPVTRWLSD
                     LDRIERGVEHITTPASFASEVYTLREHIDLVRRAVLAKSSAAN"
     gene            complement(350..1660)
                     /locus_tag="VEJ00_10800"
     CDS             complement(350..1660)
                     /locus_tag="VEJ00_10800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX01695.1"
                     /translation="MGAVLGGFVGYWNAYRAVHEGIAPTAMTVASPADAGPLSIVVLP
                     FANQTGDPQRAYIADGLTSSITADLSRIRDAFIVPVSTAITYRDKAASAKQIGQDLGV
                     HFVLQGSVMADGNKLRINAQLADTQSGAQKWTESFDGDLADLFALQDKVTARIGNSIG
                     NQMVILAARESQTRKSGARVADLLLRADALGLNAQSLQNWKDIEALQRQALAVDPGNV
                     SATKDLAFSLLVQAWNFAGVLKDDAREKLITEGGALALKAKTMDPDSPAVYGTLAMYA
                     WFQGDTAAALRYAEKRVALDPRNPDAYNNLAVIYYHLDQPAKSIEMLNRALALYPRGF
                     DALFGNLAAASLMLGDNAGAMAWVQKAIDTGTGQDRIYALLAIVYAEQGDEVRARAAA
                     AEARRRFPDMKPPTLSARECVSEVWCQYLKTKFLPAWRKAGLPQ"
     gene            2254..2535
                     /locus_tag="VEJ00_10805"
     CDS             2254..2535
                     /locus_tag="VEJ00_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01696.1"
                     /translation="MTSKQNFRSQPVILASAMLTVAACLFSAPGVRAADLNEKPWQVA
                     AADGVDKGATAVVASTSAVEVLIDSPYNVCHPARGKCAKLGGNIYFAVQ"
BASE COUNT          499 a          930 c          933 g          578 t
ORIGIN      
        1 ccgtttccta ccccagatcg cgagctggcg cgtgcgccgc ctcatctatc ggtggtacgg
       61 ccagctgatc gtgctcgagc acgacgtgca ggcgcgcgag ggtgacctgc ccgtcacgcg
      121 gtggctgtcc gacctcgacc gcatcgagcg gggcgtcgag cacatcacga ccccggcgag
      181 cttcgcgagc gaggtgtaca cgctgcgcga gcacatcgat ctcgtccgcc gcgccgtgct
      241 cgccaagtcg tccgccgcga attagggcct agccccgccg gtccagccgg cacgcccggc
      301 cggcgcagtt cggccacaac cgctcgtaag ttctcgcttc cgaaaccggt cactgcggca
      361 atcccgcctt tcgccacgcc ggcaggaatt tggtcttgag atattggcac caaacttccg
      421 aaacgcactc ccgcgccgaa agtgtgggcg gcttcatgtc gggaaatcgc cggcgcgctt
      481 ctgctgccgc ggcgcgtgcc cggacttcgt ccccttgctc ggcgtaaacg atcgccagaa
      541 gggcataaat gcggtcctgc cccgtgccgg tgtcgatggc cttctgcacc caggccatgg
      601 ctcctgcgtt atcccccagc atcaaagagg cggcggccag gtttccaaag agcgcgtcga
      661 atcccctggg gtaaagcgcg agggccctgt tcaacatctc aatcgacttt gcaggttggt
      721 caagatggta gtagatgact gcgaggttgt tgtaggcgtc cggattgcgg ggatccaacg
      781 caacgcgctt ctccgcatag cgcagcgcgg ccgcagtgtc gccctggaac cacgcataca
      841 tcgccagcgt gccgtagacc gcagggctgt cgggatccat ggtcttggcc ttgagcgcaa
      901 gcgcaccgcc ttctgtgatg agcttttccc gcgcatcatc tttgaggacc cccgcaaagt
      961 tccatgcctg caccaagagc gaaaaggcca agtctttcgt tgcagatacg ttgccgggat
     1021 cgacagccag cgcctggcgc tgcagtgctt caatgtcctt ccagttctgc agcgactgcg
     1081 cattcaggcc cagcgcgtcc gcgcgcagca gcaaatccgc caccctggcg ccactcttgc
     1141 gtgtctggct ttcacgcgcc gccagaatca ccatctggtt gccgatgctg ttgccgatgc
     1201 gcgcggtcac cttatcctgc agggcgaaga ggtcggcgag atcgccgtcg aaactctcgg
     1261 tccacttctg cgcgccggac tgcgtgtcgg ccagttgcgc attgatgcgc agcttgttgc
     1321 catcggccat gacgctgcct tgcagcacga aatgcacgcc caggtcctgt ccgatctgct
     1381 tcgctgacgc ggccttgtcg cggtaggtga tggcggtgct gactggcacg atgaacgcat
     1441 cgcggatgcg ggagagatcg gcggtgatac tgctggtgag gccgtcagcg atgtaggccc
     1501 tttgggggtc gccggtctgg ttggcaaaag gcagcaccac gatcgacagc gggcctgcgt
     1561 cagcaggcga ggcgaccgtc atcgcggtgg gcgcgatgcc ttcgtgcacc gcgcggtagg
     1621 cattccagta cccgacgaac ccgccgagca ccgcgcccat ggcggcgatg gccgcaatcg
     1681 ggcccctgag acgcttgagc cggtcgaaca gtcgcgacca acgcgcatga gcggggctca
     1741 ctttcgtctg cggctgcgac gacggtgcgg tctcgcttac ttcgctcatt cgtcaccgat
     1801 tatggaaggg gcccgggcga gggaagtata acgacgcgcg ctgacgtaag gggcccgttc
     1861 aacgtcggct tttggtgggc ttgagcgact gcaatgggtc gtgtcgcggc gttgaaagtc
     1921 cggtttgcgc ttgcctcgcc taggctatcc ttcggggcag aagcggtcgt agcgatgcac
     1981 gcttgataac agggattggc ctcgacatcg ccttgagaga ctgatcgtgc ggagcgaagc
     2041 gtgctggtcg cccgcccacc cgtcgccgct agggccgttc tgctctgtcg tgccgcattt
     2101 cgtcgcggcg ccgttgacgc acggacgctt gccgcgcaaa ttgccatccg aacgcggacc
     2161 tcggtgtttg ccccaagccg gtcgtcgtcc acttccgcgg ccctgcacaa ccggaaccgc
     2221 gctttcgtgt caccactttc caaggagtct gtcatgacct cgaagcagaa tttccgctcc
     2281 cagcccgtga tactcgccag cgccatgctc accgttgcgg catgcctttt ctcggcgcca
     2341 ggtgtccgcg ccgctgacct gaacgagaag ccgtggcagg ttgctgctgc cgatggcgtc
     2401 gacaaaggcg cgaccgctgt ggtggcctcc acatcggctg tggaagttct aatcgattcg
     2461 ccgtacaacg tgtgtcatcc ggccagaggg aaatgcgcga aactcggtgg gaacatctac
     2521 ttcgccgtgc aataggagcg aaagagtcga gcgtcgcgcc tcaatgcaat ggtgcggcgc
     2581 ttggacggcg cagatctgat aaatgcgacc ctaggtggcc cgttccgtta gccatcacga
     2641 ggtcacggtt gggcaggtca gagtcaacgg cggacgcgga ggccatcacc cgtcaggtgg
     2701 cccgcgtacg aggtaccgct actagtgggt gtccgcttgt atggtgtttt cggctttctt
     2761 ctgaaaaggt gggtcgattc gacttgaggt tatcgcgata agtcgctgat tcggcgcgtc
     2821 aggttgcgga attggagtag taaactatcg ggtgcgttcc cgcgtccagc agattcccat
     2881 cggcgcgcat gaacgcgagg aagcctattt gcagcgcgtc cgcgtagcgc tcgaccagac
//
LOCUS       DASPUL010000454         3101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_917889, whole genome shotgun sequence.
ACCESSION   DASPUL010000454 DASPUL010000000
VERSION     DASPUL010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917889"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VEJ00_10810"
     CDS             complement(<1..202)
                     /locus_tag="VEJ00_10810"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX01697.1"
                     /translation="MPDDARQKAHQLAEDHYYAALDLMADGQLEKAVAAYQESVAADP
                     MFTEAMHGLARALQDLQRYDEAL"
     gene            353..586
                     /locus_tag="VEJ00_10815"
     CDS             353..586
                     /locus_tag="VEJ00_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01698.1"
                     /translation="MRHRLRFLWNATKGHRLTPWRSPYLLWRIETYTGVKMTQIGFLE
                     FWEFMWRERRELWRFLRWTGEMERYVHPRPKSS"
     gene            complement(657..1487)
                     /locus_tag="VEJ00_10820"
     CDS             complement(657..1487)
                     /locus_tag="VEJ00_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HXX01699.1"
                     /translation="MPHRPIITLTTDFGLNDHFVGAMKGVMLDIVPDAEIVDISHAVQ
                     AFDVLDGALAIAQAYSYFPNATVHLVVVDPGVGTTRRPIIATSDGYHFVAPDNGVLSM
                     VYAREERIRVRHVTADHYFRQPVSQTFHARDVFAPVAAYLAKQVDSHKFGDEIEDYVR
                     FAAPKPKPAGENRIRGVVLKVDRFGNLITNVTPEDVPALFAAKAAPFKIVVGSKEITD
                     IHAAYAEGEPGEIFGILGSMGYLEIAANRGAAAQITGAGKGSEVSIVLGEGAAAGKGA
                     "
     gene            1590..2312
                     /locus_tag="VEJ00_10825"
     CDS             1590..2312
                     /locus_tag="VEJ00_10825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXX01700.1"
                     /translation="MPEIRGRQGAPMKLRLESRPVGEVLIVQCHGRIVGGDEVLTLHA
                     HVGDSLAKYGDVVLQLDHVEFIDSSGMGALMRLLQAARSKRGDLKLTGVPANIRKTLE
                     MTHLLQQFEVYDSVEEAITAAYLGSKYSKPKSGDARPRILCVYESSDVCTFLREVLCS
                     AGYNALATVNMDDAKILLKATKAKQVIISSRMQSIYGKPTRKALEEIDPTVSLTVLDE
                     NFATQDPGEAAEKLLNGLGKTA"
     gene            complement(2409..>3101)
                     /locus_tag="VEJ00_10830"
     CDS             complement(2409..>3101)
                     /locus_tag="VEJ00_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXX01701.1"
                     /translation="RREGDVHAIEQNPPIRQDGNRIHIDYVSMRDIAVDYEITVPADA
                     TITTHSGSGDQLIEGTHGNVDVQTGSGDVKLANLTGEVRLQTGSGDVRAHEISGTVRG
                     GAGSGDIEIEEKGPGDVDLHTGSGNIDVHGLQGGFRAEAGSGDVTAEGTQNGSWEVRT
                     GSGNVRVRLPSNAAFDADISTSSGTIDVEHPIEMTVQGRVQESRKQIRGKVGGGGALL
                     SVRTGSGDIHIQ"
BASE COUNT          599 a          962 c          934 g          606 t
ORIGIN      
        1 caagcgcctc gtcgtaacgc tgcaaatcct gcaaagctcg cgcaaggccg tgcatggctt
       61 ccgtgaacat tggatcggca gcaactgact cctgataggc cgcgacggcc ttttccagtt
      121 gcccgtccgc catcagatcg agcgcggcgt agtagtggtc ttcagctagt tgatgagctt
      181 tctggcgggc gtcgtcaggc atgcagggaa atcccaggtg gagcctggat tctagcacgg
      241 cgaatgtagg gacagccgcc ctcggctgtc cagcgggccc gtcagtttaa accggcgtgc
      301 cgcttgcctt tcctgggtgt actgccgcgc cggttgtggt tatcatgctc ccatgcgcca
      361 tcgactccgc tttctgtgga atgcgaccaa gggccatcgc ctgaccccgt ggcggagtcc
      421 ctatttgctg tggcgcatcg agacctacac cggggtgaag atgacgcaga tcggatttct
      481 ggagttctgg gaattcatgt ggcgcgagcg gcgggagttg tggcgatttc tgcgctggac
      541 gggcgagatg gagcgctacg tccatcccag gccgaagagt tcctagaggc agtggtcagt
      601 ggccagcaag acccaagatc agctgaccac tgaccactag ccactgacca ctgtttctac
      661 gcgcccttgc cggccgcggc gccctcgccc aggacgatgc tgacctcgct gcctttccct
      721 gcgccggtga tctgcgcggc cgccccgcgg ttggcagcga tctccagata acccatgctg
      781 cccagaatgc caaagatttc tcccggttcg ccctcggcgt aggcagcgtg gatatccgtg
      841 atttccttgc ttcccaccac gatcttgaaa ggggctgctt tggccgcaaa cagcgccgga
      901 acgtcttctg gcgtcacgtt ggtgatcagg ttgccgaagc ggtcaacttt cagcaccacg
      961 ccccggatgc ggttctctcc cgcgggcttt ggcttgggcg cggcgaagcg aacgtagtcc
     1021 tcaatctcgt cgccaaactt gtgcgagtcc acctgtttgg ccagataggc tgccacggga
     1081 gcgaagacat cgcgggcatg aaaggtctgg ctgaccggct gccggaaata atggtcggcc
     1141 gtgacgtgcc gtacccgaat gcgctcctca cgcgcataca ccatggagag cactccgttg
     1201 tcaggcgcga cgaagtgata gccgtcactg gtcgcgatga tcggccgccg cgttgtgccc
     1261 acccccggat ccaccacgac caagtgcaca gtcgcgttag ggaagtacga ataagcctgg
     1321 gcgatggcca aggctccatc gagcacatcg aaagcctgca cggcgtggga aatgtcgaca
     1381 atttcggcgt caggcacgat gtcgagcatg acgcccttca tcgcgcccac aaagtggtcg
     1441 ttaagcccaa agtcggtggt cagagtgatg atcggacggt gcggcaaggg ttcctcgctg
     1501 gaagacaata tgacgacaga cggaatactc cccgtaactg cgaagttaat ttgcagaaag
     1561 cttgtgcgtc aaggcacgga ggtaatagga tgccagagat tcgcggcaga cagggggcac
     1621 cgatgaagct gcgtctggaa agccgacccg taggcgaggt tctgatcgtg caatgccacg
     1681 ggcgcatcgt gggtggagat gaagtactga ccctgcacgc gcacgtcggc gactccctcg
     1741 ccaaatacgg cgacgtcgtg ctgcaactcg accacgtgga gttcatcgac agcagtggca
     1801 tgggagcgct catgcgcctg ctgcaggctg cgcgttccaa acgtggcgac ctcaagctga
     1861 ccggagtgcc ggccaacatc cgcaagaccc tggagatgac ccacctgctt cagcaattcg
     1921 aagtttacga ttcggtggag gaagcgataa cggccgcgta tctgggttcc aagtattcca
     1981 agcccaaatc cggcgacgcc cggccgcgca tcctgtgcgt gtacgagtca tccgacgtct
     2041 gcaccttcct gcgcgaggtg ctctgcagcg cgggttacaa cgccctggcc acggtgaaca
     2101 tggacgatgc caagatcctg ctcaaggcaa ccaaggccaa gcaggtaatc atttcttcac
     2161 gcatgcaatc catctacggg aagccgacgc ggaaggcgct ggaggaaatt gatcccaccg
     2221 tctcgctcac cgttctcgac gaaaacttcg cgacgcagga tcccggggag gcggcggaaa
     2281 aactgctgaa tggcctggga aagacggcgt agccgaactt ctacgccacg gattcacgcg
     2341 gatcgaacgg ataaagccat caatggatcc gtgaaaaccg tgtatatccg tggcaagcga
     2401 agttgatctt actggatgtg aatatcgccc gagccggtgc gcaccgagag cagcgcgccg
     2461 ccgccaccga ccttgccgcg aatctgcttg cgcgactcct gcacgcggcc ctgcaccgtc
     2521 atctcgatgg gatgctctac atcgatcgtg cctgaactgg tagagatgtc agcgtcaaac
     2581 gcggcgttcg acggcaggcg cacccgcaca ttgcccgaac cggtgcggac ttcccagctc
     2641 ccattctgcg tgccctcggc ggtcacgtcg ccgctccccg cctcggcgcg aaatcctccc
     2701 tggaggccat gcacgtcaat attcccggag cccgtgtgca gatcgacgtc tccggggccc
     2761 ttttcttcga tctcaatatc gccgctgccc gcgccgcccc ttaccgttcc tgagatctca
     2821 tgcgccctga catcgccgga gccggtttgc agccggactt cgccggtcag attcgccagc
     2881 ttgacgtccc ccgaaccggt ctgcacgtcc acgtttccgt gcgtgccctc gatcagctgg
     2941 tcccccgaac cgctgtgtgt ggtaatcgtg gcgtcagccg gcacggtgat ctcatagtcg
     3001 actgcgatat cgcgcatgct gacgtagtcg atgtgaatgc ggttgccgtc ctgccggatg
     3061 ggagggttct gttcgatcgc gtgaacatcg ccttcgcgcc g
//
LOCUS       DASPUL010000455         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_226305, whole genome shotgun sequence.
ACCESSION   DASPUL010000455 DASPUL010000000
VERSION     DASPUL010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226305"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..774
                     /locus_tag="VEJ00_10835"
     CDS             217..774
                     /locus_tag="VEJ00_10835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXX01702.1"
                     /translation="MDLDSKSLQAPDFETLTLQHKDSVYRQMIRTCGNREDAEDVLIE
                     ALHSAYRHLDQLRQSSAFRAWLAQIARRVCWQLKEREALRPLLQLSDLGEEGREIPGS
                     APTPEVQLARREMKQLLQEAVDALPPLYRSVYALRDLEDLAGEEVARRLGISSAAMKS
                     RLLRARNLIREHLDAALTRENLKNS"
     gene            793..1203
                     /locus_tag="VEJ00_10840"
     CDS             793..1203
                     /locus_tag="VEJ00_10840"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXX01703.1"
                     /translation="MEVAIEHLGAVQFEIKARQHSISCDQPPENGGYDEGMTPPELLL
                     ASLGSCAAFYAAQYLRKHKLATEGTRVRIVADKVKDPARVDNFRIEIETPVSLSEQHR
                     AGVEASVHHCLIHNTLLHPPKIAIEIKEQVPLPQ"
     gene            complement(1219..1839)
                     /locus_tag="VEJ00_10845"
     CDS             complement(1219..1839)
                     /locus_tag="VEJ00_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01704.1"
                     /translation="MRAEHREVPLDSGTLSDGVYRNKSLGLSCKIPLGWVLRTEEMNA
                     REDGEGDVIPSPSAQDPHFSQNRGEVGHPAGGRVLLATFSRPPEARGEDVNSSILIVA
                     EDAAAYPGLKEAAQYFGPLTEVAKAQGFSVDEEPYEVAVGTKTLVRGDFHKDVGPRVM
                     RQSTLAMLAHGYAVSITVIGGTEDEVEELIEGLDFAGSARGAKAPK"
     gene            complement(2078..>2564)
                     /gene="mscL"
                     /locus_tag="VEJ00_10850"
     CDS             complement(2078..>2564)
                     /gene="mscL"
                     /locus_tag="VEJ00_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXX01705.1"
                     /translation="APLDLSAPLPYSGGHFNLGGGDVMLSGFKKFILRGNVVDMAVGV
                     VIGAAFGGVVAALTKDLLTPLIAAIVGKPDFSAISFTINGTVFPVGDFINACVSFLLI
                     AAAIYFFVVTPVNALVARMRKDPAPADPTTKKCPECLSEIPIDARRCAHCAQPQVGKA
                     A"
BASE COUNT          533 a          836 c          741 g          454 t
ORIGIN      
        1 gtccacccag cctggctctt cctgtcaatg ttcgtcggcc tgggcctgac atttgcgggg
       61 gttaccgacc tatgcctgat gggtgaggtg ctcgggagaa tgccctggaa tgaacaaagt
      121 cactgcaaag tggctcatcc caaagcagaa ccatgcccgg gcaagcagtg agcgaaaaca
      181 cagcggcggg gtgcgcggca atgccagaat ggctaggtgg atctcgacag caaatcgctc
      241 caagcgcccg atttcgaaac actgaccctg cagcacaagg actcggtcta tcggcagatg
      301 atccgtacct gcggcaatcg cgaggacgcg gaagacgtgc tgatcgaggc cctgcacagc
      361 gcttatcgcc acctcgacca gctccgccag tcgtcggcgt tccgtgcctg gctggcgcag
      421 attgcgcgcc gggtctgctg gcaactgaag gaacgcgagg cgctgcggcc gttacttcag
      481 ctctccgatc tcggcgagga aggccgcgag atccctggaa gcgcgcccac tccggaagtc
      541 cagctggcgc ggcgcgagat gaagcaactg ctccaggagg cggtcgacgc actgccgccg
      601 ctttatcgct cggtgtacgc gcttcgcgac ctcgaggatt tggcgggcga agaagtggcg
      661 cggcgtttgg gaatctcatc agccgccatg aaatcgcgcc tgctccgcgc ccggaatctg
      721 atccgggaac atttggacgc agccctcacc cgcgagaatt tgaagaattc ttagtcaatt
      781 tgggaggcac ttatggaagt ggcaattgaa catctgggag cagtacagtt cgaaatcaaa
      841 gcccgccagc actcaatctc atgcgaccag ccgccggaaa acggtggcta tgacgagggc
      901 atgaccccgc cagagttgct cctcgcttct ctcggctcct gtgccgcgtt ctatgccgcg
      961 caatatctgc gcaagcacaa actcgccacc gaggggactc gagttcgcat cgtcgccgac
     1021 aaagtgaaag acccggcgcg agtggacaac ttccgcatcg aaatcgaaac cccggtcagc
     1081 ctgagtgagc agcatcgcgc cggagtcgag gcctcggttc accactgcct gatccacaac
     1141 acgctgctgc acccgcccaa aattgccatc gaaatcaagg agcaggtacc gctgccgcaa
     1201 taacctggcg ggctggactc acttcggcgc cttcgcgcct cgcgccgatc ccgcgaagtc
     1261 cagcccttca attagttctt caacctcgtc ttcggtcccg ccgatcacgg tgatcgacac
     1321 agcgtaaccg tgcgccagca tcgccagcgt agactggcgc atcacccgcg gtcccacatc
     1381 tttgtgaaag tccccgcgca ccagcgtctt cgttccgacc gcgacctcgt acggctcctc
     1441 gtcgacgctg aacccctgtg ccttcgccac ctcagtcagt ggcccgaagt attgcgccgc
     1501 ttctttcaag ccgggatatg ccgccgcgtc ttccgccacg atcaggattg acgaattcac
     1561 gtcctcgcca cgcgcctccg gaggccgcga gaacgttgcc aacaggaccc tgccaccggc
     1621 cgggtgcccc acttctcccc ggttttggga gaagtggggg tcttgggctg aaggactcgg
     1681 aatcacgtcg ccctccccat cctcccgcgc gttcatctcc tctgttcgca gcacccagcc
     1741 cagcggaatc ttgcaggaaa gtccaagaga cttgttgcga taaacgccat cgctcagcgt
     1801 tccggaatca agcggaacct cccgatgttc agcccgcacc tttcgcgcag acggagagga
     1861 cgcgggggat tgcgctgaag aaggcacaag gaaggtcagc gggaatagaa ccagaacgaa
     1921 aacgatgccg caccttcgcc caaacttcat ccagctatgg taacggattc cggtggaagc
     1981 tcgggcgcgg gaaggaacga gaaaggagat gggtgcccca tcctatcgcg ctctttgcga
     2041 tagggtgggg ccctttccac tgcagtcggg aattctgtta cgccgccttc cccacctgcg
     2101 gctgggcgca gtgcgcacag cgccgcgcgt cgatgggaat ctcgctcagg cactccgggc
     2161 acttcttggt cgtgggatcc gccggcgccg gatctttccg catgcgcgcc accagcgcat
     2221 tcaccggcgt caccacgaag aagtaaatcg ccgccgcgat caaaaggaac gatacgcagg
     2281 cgttgatgaa gtcgcccacg ggaaacacgg tcccgttaat cgtgaagctg atggccgaaa
     2341 agtccggctt gcccacgatc gccgcgatca gcggcgtcag caggtccttg gtcagagccg
     2401 caaccacccc gccgaacgcc gcgccgatca ccacgcctac cgccatatcg acaacattgc
     2461 cgcgaaggat gaactttttg aatccgctta acataacgtc gccccctccc aaattgaagt
     2521 ggccgccact gtacggcaga ggcgcagaga ggtcaagggg agcc
//
LOCUS       DASPUL010000456         6579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_918320, whole genome shotgun sequence.
ACCESSION   DASPUL010000456 DASPUL010000000
VERSION     DASPUL010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918320"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VEJ00_10855"
     CDS             complement(<1..578)
                     /locus_tag="VEJ00_10855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HXX01706.1"
                     /translation="MTQSKKDSKARWRHYDFTTAGPVDDYIYSMLPKRDDVLAEMEDY
                     ATKHNVPIVGPAVARVLQQLAMMIGARMVFELGSAIGYSTIWWAQAVGEKGRVIYTDG
                     DSKNAERARGYFSRAGVSNRVQLHVGDALEVLSEQKQEFDVIFNDVDKEDYPRVLRLV
                     APRLRKGGLFVTDNVLWSGRVAEKSPTDARTRA"
     gene            complement(615..1319)
                     /locus_tag="VEJ00_10860"
     CDS             complement(615..1319)
                     /locus_tag="VEJ00_10860"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXX01707.1"
                     /translation="MNLKGIKLTWLGHATFRIETPAGKTVIIDPWIMGNPMCPQAERK
                     VKKADILLCTHGHGDHIGDAVEIIKQHNPIVVGIPELARWLGKKGARQVAEMNKGGTQ
                     AVGDIKVTMVHADHSCGIQDGDQLIYGGEACGYVIEFSTGTKIYHAGDTNVFGDMSII
                     RELYAPEIVMLPVGDHYTMGPREAAYACNLLKPKTVIPMHFGTFPVLVGTPAELRKLV
                     KGAEVLEMTPGVTIGS"
     gene            1413..1964
                     /gene="hpt"
                     /locus_tag="VEJ00_10865"
     CDS             1413..1964
                     /gene="hpt"
                     /locus_tag="VEJ00_10865"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HXX01708.1"
                     /translation="MADLKVLISREDIAKRVGELGAQITKDFTGQSVILVGVLKGAAI
                     FLSDLARHIDLDATFDFIGVSSYGNRPSPKQELKSGWDSTGEVKLTKDVDQSMKDKNV
                     IVVEDILDTGLTLTVLKKLLLAHQPKTLKIAALLDKPSRRKLPLEGDYVGFKIPDEFV
                     VGYGLDYAEKYRNLADICVVPRE"
     gene            2025..3797
                     /locus_tag="VEJ00_10870"
     CDS             2025..3797
                     /locus_tag="VEJ00_10870"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXX01709.1"
                     /translation="MGSALAKPDQLPGESLLLDVADVLATSLDLDTTVRRVAEVVRKV
                     IDYEIFAILLLNEKTQELRFRFQVGYPPEFAERTRIKVGEGVTGQAAQLRQAILIDDV
                     TRDPRYIAAVPNVRSELAVPLITKNRVIGVIDIEAREPGYFTEEHKRLLTLVASRMAA
                     GIENAQLYTRTTKQARILLLLNEISRELTSILNLDELLGRIAELVRRLIDYQMFSILL
                     LDASGEKLQHRFSLRFKESVHLKHEIPLGRGLVGTAAASGQAVLVPDVSKDPRYIEAN
                     PETRSELAVPLTYKGKVIGVLDLEHTRRGFFTDEHQRTMTTLAAQIAIALENARLYEE
                     IERQERRLERDLALARELQVRLLPQASPRLAHLDVAAKFVPARAIGGDLYDFIPYSLS
                     RLGIAIGDVSGKGAPAAIYAALVSGILRSHAPIEPSPAEMLSAVNLSLAERRIEAQFV
                     SIIYAVWDDQHRTLTVANSGLPRPIYVHGGKNEVIEATGLPLGLFDEADYDEFTFRMK
                     PGDMFVFFSDGILDARNRQGQLFGRRGVERVIAECTAQSAECVVNSLFQAVAEHAAGV
                     ETFDDQTVVAIHVKGSAHASPKKK"
     gene            3794..5122
                     /gene="lysA"
                     /locus_tag="VEJ00_10875"
     CDS             3794..5122
                     /gene="lysA"
                     /locus_tag="VEJ00_10875"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HXX01710.1"
                     /translation="MTAGRQNLPERPPGFTFRPSKRGILRRGEPFVLHCENIALPKLA
                     EHYGTPLYVYSAATIRERCAAFAGAFRRVPNLICYSVKANSNLSILRLLAKEGCGFDV
                     VSGGELERVLTADRKAAKKVVFSGVGKTREELTAALKAGILLFNVESESELWALAECA
                     GRMRTAAPLAVRVNPDVKAETHPYISTGLHEHKFGVPIRDARALYAKASGARYLKVRG
                     VSVHIGSQITDVAPFAEAMARVADLVRELRADGHSIDLVDAGGGLGISYERAAAADFS
                     SYAESYARALAAPLQGLDVRLLLEPGRSIVGPAGILLTSVLYKKRNDGKKFLVVDAAM
                     NDLLRPALYGAYHEIVSARQNPDPESKVEVVDIVGPVCESGDFLARDRQMPDIAEGDL
                     LAILDAGAYGMVLSSNYNTRPRPAEVLVSGKTTKLIRRREKVSDLLRAEL"
     gene            complement(5132..6040)
                     /locus_tag="VEJ00_10880"
     CDS             complement(5132..6040)
                     /locus_tag="VEJ00_10880"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HXX01711.1"
                     /translation="MGRPKASSRKNSRAKSTTQKTAAIISRPDGPEVAKAVPGLLSWL
                     VERGYKVIVDQETAPHAGQQEVVPRGQMAARPLDLVVVLGGDGTLLSAARATAAIDVP
                     LLGVNLGSLGFLTEVPLPSLYPMLEAIRQGRAAVEHRSLMRCELLRGKTVRGSYLVFN
                     DAVVNKTALARLNNYDLYIDKAFVSSYRADGMIVATPTGSTAYSLSAGGPILAPTVSA
                     FVITPVAPHALTHRPLVVPDSVEIELTLRSAEEVAYLSLDGQPGLDLRDGDRVRCRRS
                     EHQVTLFRTGTDFYQALRTKLKWGEG"
     gene            complement(6113..>6579)
                     /locus_tag="VEJ00_10885"
     CDS             complement(6113..>6579)
                     /locus_tag="VEJ00_10885"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXX01712.1"
                     /translation="CLLQHGAARVIAVDTGYGQIDLKLRQDPRVRLLEKTNARYLTRD
                     AVGETVDIVVTDVSFISATLVLPAVVSAAFPESREERRGKRVVVLVKPQFEAGREHVG
                     KGGVVRDEAAQTAAVERVTAALKDLGIARADVIDSPILGAEGNREFLLYGEF"
BASE COUNT         1277 a         2096 c         1909 g         1297 t
ORIGIN      
        1 gccctggtcc tggcatcggt cggactcttc tccgcaaccc gcccgctcca cagcacgttg
       61 tcggtcacga ataatccgcc tttgcgcaac cggggtgcaa ccaggcgcaa cacgcgcgga
      121 tagtcctcct tatctacgtc gttgaagatg acgtcgaact cttgcttctg ctcggagaga
      181 acttcgagcg catcgccgac gtggagctgc acacggttgg agaccccggc gcgcgagaaa
      241 tatccgcggg cgcgctcggc gttcttcgaa tcgccgtcgg tgtagatgac gcggcctttc
      301 tcacccaccg cctgcgccca ccagatcgtg gaatagccaa tggcggagcc caactcgaag
      361 accatacggg cgccgatcat catggccaac tgctgcagga cgcgcgccac cgccgggccc
      421 acgatcggca cgttgtgctt ggtggcgtag tcttccattt ctgccagcac atcgtcgcgc
      481 ttcggcagca ttgaatagat gtagtcgtca accggccccg cggtggtgaa gtcgtagtgg
      541 cgccacctgg ctttgctgtc tttcttgctc tgagtcacac gtttcccttt cgcgagagaa
      601 tcttcggttt gagtttaact accgattgtc acacccggcg tcatctcgag tacctccgcc
      661 cccttgacca gcttccgcag ctccgccgga gttccgacca gtacgggaaa tgtgccgaag
      721 tgcatcggga tcaccgtctt cggcttcagc agattgcatg cgtacgccgc ttcacgcggt
      781 cccatggtgt agtgatcgcc caccggcagc atcacaatct ccggcgcgta cagttcgcgg
      841 atgatcgaca tatccccgaa tacgttggtg tcgccggcat gataaatctt cgtgcccgta
      901 gaaaactcga tcacgtaccc gcaggcttcg ccgccgtaga tgagctggtc tccatcctgg
      961 atgccgcagg agtgatccgc atgcaccatc gtgactttga tatcgccgac ggcctgcgtg
     1021 ccgcctttgt tcatctcggc gacctgcctg gcgcctttct tgcccagcca tcgcgccagt
     1081 tcggggattc ccaccacaat cgggttgtgc tgcttgatga tctcgaccgc gtctccgatg
     1141 tgatcgccgt gcccatgggt gcaaagcaga atatccgcct ttttcacctt cctctctgct
     1201 tgcggacaca tgggattgcc catgatccac ggatcgatga tcacggtctt acctgcgggc
     1261 gtttcgatgc gaaaggtggc gtggcccaac caggtcagtt tgatgccttt caggttcatg
     1321 cgaaaaccag ctccttggac gaatgctaaa acggtcgtaa gagattgccc acgcaccctt
     1381 tgagttgacg gactagttta atatgaatcg ccatggccga tctcaaggtg ctgatttcgc
     1441 gcgaggacat cgcaaagcgc gtgggcgaac tgggcgctca gatcaccaaa gactttaccg
     1501 ggcaatcggt gatcctggtg ggcgtgctca agggggccgc gatttttctt tctgatctgg
     1561 cgcggcacat cgatcttgac gccaccttcg actttatcgg ggtatcgagt tacggtaacc
     1621 ggcccagtcc gaaacaggaa ctcaaaagcg gatgggattc caccggcgag gtgaaactga
     1681 cgaaggacgt cgaccagtct atgaaggaca agaacgtcat cgtggtcgaa gacattctcg
     1741 acaccgggct gacgctcacg gttctgaaga agctgctgct ggcgcaccag ccaaagactc
     1801 tcaagatcgc ggcgctgctc gacaagccgt cgcgccgcaa gcttccgctg gagggagact
     1861 atgtcgggtt caagattccc gacgaatttg tcgtcggcta tggactggac tacgccgaaa
     1921 agtaccgcaa ccttgccgac atctgcgtag ttcccagaga atagttgcta ctgggcgatc
     1981 tgctgcagcc gaaggggcga agtgcggctg ctatcatgaa aagaatgggt tctgcccttg
     2041 ccaaaccgga tcagttgccg ggcgagtcgt tgctgctcga cgtagccgac gtgctggcca
     2101 cctcgcttga cctcgacaca accgtgcgcc gtgtcgccga ggtcgtgcgc aaggtcatcg
     2161 actacgaaat ctttgcgatt ctgctgctca acgaaaagac gcaggaactg cgcttccgct
     2221 tccaggtcgg ctatccgccg gagtttgccg agcgcacgcg gatcaaagtg ggagaaggtg
     2281 tcaccgggca ggcagcccaa ctgcgacagg ccatattgat cgacgacgtg acccgtgacc
     2341 cgcgctacat cgcagccgtc cccaatgtgc gctcggagtt ggccgtgccg ctcattacca
     2401 agaaccgcgt catcggcgtc atcgacatcg aggcccgcga gcccggctat ttcaccgaag
     2461 aacacaaacg cctgctgact ctggtcgcgt cgcgcatggc ggccggcatc gagaacgcgc
     2521 aactttacac ccgaacgacc aagcaggcgc gcattcttct gctgctcaac gagatttcac
     2581 gcgagctgac ttcgatcctg aacctcgacg agttgctcgg ccgcatcgca gaactagtgc
     2641 gcaggctcat cgactaccag atgttcagca tcctgctgct ggatgcgtcg ggggagaagc
     2701 tgcagcaccg tttttccctg cgcttcaagg agagcgttca cctgaaacac gaaatccctc
     2761 tcggccgcgg ccttgtcggc acggcggcgg cgagcggaca ggctgtgctc gttcccgacg
     2821 tcagcaagga tccgcgctac atcgaagcca atcccgagac tcgttccgag ctggcggttc
     2881 ccctgaccta caagggcaag gtgatcggtg tcctcgatct ggaacacacg cgccgcggat
     2941 tttttacgga tgagcaccag cgcaccatga ctaccctggc cgcgcagatc gcgattgccc
     3001 tcgagaacgc gcgcttgtat gaagagatcg aacgccagga gcggcgactg gagcgcgatc
     3061 ttgcgctggc gcgtgagttg caagtccgcc ttctgccgca agccagcccc aggctggccc
     3121 acctcgacgt ggcggcgaaa ttcgttcccg cccgcgcgat tggcggagat ctttacgatt
     3181 tcatccccta ctctctctcg cggctgggca tcgccatcgg cgacgtcagc ggcaagggcg
     3241 caccggctgc gatctacgcg gcgctggtca gcggaatcct gcgctcgcac gccccgatcg
     3301 aaccgagccc ggccgagatg ctatcggccg tgaacctgtc cctggctgaa cgccgcattg
     3361 aggcgcaatt cgtctccatc atctacgcgg tgtgggacga ccagcaccgc accctgaccg
     3421 ttgccaactc gggtcttccc cgccctatct atgtccacgg cggaaagaac gaggtcatcg
     3481 aggccacggg actgccgctt ggcctgttcg acgaggccga ctacgacgag ttcaccttcc
     3541 gcatgaagcc cggcgatatg ttcgttttct tcagcgacgg catcctcgac gcccgcaacc
     3601 gccagggcca actcttcggc cgccggggag tggaaagagt tatcgccgaa tgcacggccc
     3661 agtctgccga atgcgtggtc aattccttgt tccaggcggt cgccgagcac gcggccggcg
     3721 tggaaacctt tgacgatcag acagtggtcg ccattcacgt gaagggcagc gcgcacgctt
     3781 ctccgaaaaa gaaatgaccg caggccgtca gaatcttccc gagcgtccac ccggattcac
     3841 gtttcgcccg agcaagcgcg gaatcctgcg ccgcggagaa ccgttcgtat tgcactgtga
     3901 aaatattgcc ctgccgaagc tcgccgagca ctacgggaca ccactctatg tctattcggc
     3961 agcgacgatt cgcgagcgtt gcgcagcttt tgccggagca tttcgccgtg tgcccaactt
     4021 gatctgctac tcggtcaagg caaattcaaa tctcagcatc ctgcgcctgc tggcgaaaga
     4081 aggctgcggg tttgacgtgg tttctggcgg agaactcgag cgcgtcctca ctgcagaccg
     4141 caaagccgcg aagaaagtcg ttttctccgg cgtcggaaag acgcgcgagg agctgactgc
     4201 ggcgctcaag gctgggatcc tgctgttcaa tgtcgaaagc gaatcggagc tttgggcatt
     4261 agccgagtgc gcaggccgga tgcggacggc ggcaccgctc gcggtccggg tcaatccaga
     4321 tgtaaaggct gaaactcatc cctacatctc gaccggcctg catgagcaca agttcggcgt
     4381 gcccattcgc gatgcccgcg cactctacgc caaagcatca ggggcgcgct atctcaaggt
     4441 gcgcggtgtc agtgttcaca tcggatcgca gatcaccgat gtcgctccct tcgctgaggc
     4501 tatggcgcga gttgccgatc tggtgcgtga actgcgcgct gacggacaca gcatcgactt
     4561 ggtcgacgcc ggtggaggcc tgggcatttc ttacgaacgc gctgctgccg ccgacttctc
     4621 ctcctatgcc gagagctatg cgcgcgcgct ggctgctccc ctgcaagggc tcgacgtgcg
     4681 cctgctgctc gagcccggcc gctcgattgt gggccctgcc ggaatcttgc tcacgtccgt
     4741 cctctacaag aaacggaatg acggaaagaa atttcttgtg gtggatgcgg ctatgaatga
     4801 tctgctgcgg cctgccctgt atggcgccta tcacgagatt gtttcagcga ggcaaaaccc
     4861 ggacccggag tcgaaggttg aagtagtgga tattgttggc ccggtgtgtg agagcggtga
     4921 tttcctggcg cgcgatcggc agatgccgga catcgcagaa ggcgacctgc tcgcgattct
     4981 cgacgccggc gcctacggaa tggtgctgtc gtcgaactac aacacgcgcc cgcggccggc
     5041 tgaggtgttg gtgtccggca aaaccacgaa gctgattcgc cgacgagaga aagtatccga
     5101 cctgctgcga gctgagttgt agacgagcca gctatccttc tccccacttc agcttggtac
     5161 gcagcgcctg atagaaatcc gtgccagtgc ggaacagagt cacctgatgc tccgatctcc
     5221 ggcagcgcac gcggtcgcca tcgcgcaggt cgaggccggg ctggccgtcg agactgaggt
     5281 acgcaacttc ctcggcgcta cgcagcgtga gttcgatctc caccgaatcc ggcactacga
     5341 gcggacgatg agtgagcgca tgcggcgcca ccggagtgat cacgaatgcg ctgaccgtgg
     5401 gcgccagaat tggtccgccc gccgacagcg agtaggccgt agaccccgtc ggcgtagcca
     5461 caatcatgcc atcggcgcga tagctggaaa cgaacgcctt gtcgatgtag aggtcgtaat
     5521 tgttcaggcg tgccagcgcc gtcttgttca ccaccgcgtc gttgaacacg agatagctgc
     5581 cgcgcaccgt cttgccgcgt agcaactcgc agcgcatcag tgagcgatgc tccaccgctg
     5641 cccgcccctg ccggattgct tccagcatcg ggtacagcga gggcagcggc acctcggtca
     5701 gaaatccaag cgaacccagg ttgaccccga gcagcggtac atcgatggcg gccgtcgctc
     5761 tcgccgccga cagcagcgtg ccgtcaccgc ccagcacgac gaccagatcc agcggcctcg
     5821 ccgccatttg tccccgcggc acgacttcct gctgacccgc atgcggcgct gtttcctgat
     5881 cgactataac cttataaccg cgctcgacca gccagctcag aagtccggga accgccttcg
     5941 ccacctccgg accgtccggc cgcgaaatga tggcagcggt cttctgcgtg gtagattttg
     6001 ctcgggagtt tttccgggaa gatgctttcg ggcgccccat ggcagtattg aaacaacgat
     6061 tgtacagaat ctcccgctgc gaaccacagt gcgcggtcag aaccgccgcg gactagaact
     6121 ctccgtagag aagaaactcg cgattgcctt ccgccccaag gatcggcgag tcgatcacgt
     6181 ccgcgcgcgc tattccgaga tctttcaagg ccgccgtcac ccgctctacg gccgcggtct
     6241 gtgccgcctc gtcacgcacg acgcctccct tgccgacatg ctccctgccc gcttcgaact
     6301 gcggcttgac caaaacaaca acgcgtttcc cgcgcctctc ctcccgcgat tcaggaaaag
     6361 ccgcgctgac caccgccgga agcacaaggg tcgcggaaat gaacgacaca tccgtcacga
     6421 cgatgtctac cgtttccccg accgcatccc gtgtcagata ccgggcgttc gtcttctcca
     6481 gcagccggac tcgtggatct tggcgaagct tcaagtcgat ctgcccatat cccgtatcca
     6541 cggcaatcac acgcgcggcc ccatgctgaa gcaggcaat
//
LOCUS       DASPUL010000457         9621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1584351, whole genome shotgun sequence.
ACCESSION   DASPUL010000457 DASPUL010000000
VERSION     DASPUL010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9621)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584351"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /gene="yajC"
                     /locus_tag="VEJ00_10890"
     CDS             <1..296
                     /gene="yajC"
                     /locus_tag="VEJ00_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXX01713.1"
                     /translation="GGGGMGWLGIAPLIFIFAIFYFLLILPQQRRQKKWQAMLDQLKT
                     GDKVSTSGGLRGTIISMKDDAVQLRIPPDNLRVEVTKASIVHVTTTEEEGKGK"
     gene            397..2019
                     /gene="secD"
                     /locus_tag="VEJ00_10895"
     CDS             397..2019
                     /gene="secD"
                     /locus_tag="VEJ00_10895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXX01714.1"
                     /translation="MNKPLLWRAGIIVATLLIFLFGIFGIPKSFSPQGMIASMGEHIH
                     LGLDLRGGTHLILQVQVNDAVNVDAQNAMEVLREQMRNRKIDYTDITQPDQQNNPDHI
                     VLKGVQASSRTDLLNIVSDRLPEYDITGGADNSWNMSMKPSLLTDLKNKAVTQAIETI
                     RNRVDQLGVSEPQIEEHGLGQYQILVQLPDVDDPARVKDIIQNTAMLEFKQGLGGPYP
                     SEQAALQAQPTPGLLPPDAILVPGHSMPGTGEEGPAWYLVSRVSAVTGKDLRDAQPSR
                     DPNGQPSVSFTLTGDGGQRFYNFTSAHVGDPLAVVLDNKVQEWANIKEPIRDRGEISG
                     GRMSEQQSKDLSMVLRSGALPAGIRYLEERTVGPSLGADSIRAGVRAAIAGMLAVLIF
                     MLIYYRGAGINADVALILNLIILLGFMGYFGAVLTLPGIAGVILTVGMGVDSNVLIFE
                     RIREELRNGKTPPSAVDQGFSHAWVTIVDTHVTTIVSAAILFLFGTGPVKGFAVTLTF
                     GLLANLFTAVFVSRAIFDWILSRKQRGEALSI"
     gene            2144..3358
                     /gene="secF"
                     /locus_tag="VEJ00_10900"
     CDS             2144..3358
                     /gene="secF"
                     /locus_tag="VEJ00_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXX01715.1"
                     /translation="MEFFHDVNIDFLGKKWYFLAFSLIFSVAGVLSMLFWHGIPWGVD
                     FRGGTLVYVKFSHTPDLGVVRTLMDRAGLRDPKIQTYGPPSNNEVLIDLSQKETGEQA
                     LGQGKTQIINALETNAPAGKQCSAVGNSVGACDLNNASSLTIKNYLMDKDPLHLGTDA
                     DQKYTAQARDIVDYRDKVQGGVLSSIDQLKASSTDPAVVATLGDGFFLSDFGVRNVEI
                     VGPTVGAQLQKQAALATIYSLAGMLVYLGFRFEWIYGVAAVVTVFHDTLITVGAFSLT
                     KTDISLTVIAAILTLIGYSNNDTIVVFDRIRENIKLMRREKLADVVNRSINQTLSRTI
                     LTAGLTFLTVLALFLFGGEVLHGFSLALVIGILIGTYSSIAIAAPILVAYQDWRINRG
                     KRPVAMPAAKGR"
     gene            3531..4271
                     /locus_tag="VEJ00_10905"
     CDS             3531..4271
                     /locus_tag="VEJ00_10905"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXX01716.1"
                     /translation="MFEDSLIESGGRLRTKRGRTSAIAFFVQICIIVVMILIPLIFTE
                     ALPRTQLMTFLVAPPPPPPPPPPAAAPVKIVKVIQTDIVNGQLRTPTKIPQKVQMIKE
                     DEAPPPVMAATGVVGGVPGGVPGGSMGGVIGSVLSSTPVAVPKIATPQRVRVSSGVSQ
                     GLLVRRVNPTYPPLARQARIQGVVILQAQISKAGDIENLQLISGHPMLAPAAIEAVKQ
                     WKYKPYLLNGEPVEVDTQVQVNFTLSGG"
     gene            4537..5298
                     /locus_tag="VEJ00_10910"
     CDS             4537..5298
                     /locus_tag="VEJ00_10910"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXX01717.1"
                     /translation="MFATYVAGAILSHVPVAASWVMQEGGTLATDPLSLWKAMGWVAR
                     TVVIILFIMSGWSIGVMIDRWMAYSAARKQSRAFAPAVAGALRDGRIDEAIKVAERNK
                     KSHLAKVVTAGLMEFKAHQDSPGAIPGETIEASKRALERTEAIVHAELKRGLGGLATI
                     GSTAPFVGLFGTVMGILNAFIGINTSKATGLAAVAGGIAEALVTTAIGLLVAIPAVMM
                     FNYLTGRVEAFDVEMDNSSSELIDYFLKKRGDVRR"
     gene            5451..5912
                     /locus_tag="VEJ00_10915"
     CDS             5451..5912
                     /locus_tag="VEJ00_10915"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXX01718.1"
                     /translation="MSMAKRDEGKKINSDINVTPMVDVMLVLLIIFMVVTPMLQKGVS
                     VDMAKVNNPTPMEDADKEDALLVSVTRDGKIYFGSDLITIDGLTTKVKDRLANKPDKR
                     VYVKADMRARFGGVVEVVDAVRAAGVDDLGLLTEQKKTAPGAPPAAAAPGQ"
     gene            5925..6365
                     /locus_tag="VEJ00_10920"
     CDS             5925..6365
                     /locus_tag="VEJ00_10920"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXX01719.1"
                     /translation="MGMGVSAGGGQSANINVTPLIDVLLVLLIIFMVISPVTPKGLDA
                     LVPQPPPPNAPKNTTPDRTIVVQLIDRGAGTEPGLKVNQEDATWDNLQGRLEDIYKQR
                     AEKVMFVKGDDSIPFGNVANVIDIAHAAGVDKVGLITAKIEAGN"
     gene            6523..7365
                     /locus_tag="VEJ00_10925"
     CDS             6523..7365
                     /locus_tag="VEJ00_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX01720.1"
                     /translation="MNKNLRLLTLAAVALALFSSVGCNKLRARDQLNKGVEAYKNSHY
                     EQAIDHFQQAVQLDPSLINARMYLATAFVSQYIPGVDSPDNLRTAQQAIDEYQKVIDA
                     NPSRDQKVNSAKGIAYLYLNMKRWDDAKKYYRMASDLDPNDPEPYYSVGVIDWTACYQ
                     PRMEERAKLGMKPEEHLNPKNKDQKKACDELKTKNAPAIQEGIDSLNKAIQLRPDYDD
                     AMAYMNLMYRERADVECDDLSAREEDLKAADHWVDETLRVKKAKAEKEAEKAGGGITM
                     DQSK"
     gene            complement(7466..9058)
                     /locus_tag="VEJ00_10930"
     CDS             complement(7466..9058)
                     /locus_tag="VEJ00_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01721.1"
                     /translation="MADPQIVSVMRSDVSPNGGATHRVRSSGVRQAHWATLLLLFAAA
                     LAFRFACLACKPFWFDECFSVEVTRMKWPSFLHVLWWREANMALYYGLLRLWLHFGWS
                     AFFIRSLSALIAAATLPAIYWLAGLLFSERDNGRLVALIATALFAINAFHVRYAQEAR
                     SYALLVLLATLSSGFLVAAVREPRRPLGFKYMLTSVLAAYAHLYALLLIVAQWLTVWR
                     LGLPPGNANESRKTELKRAWKVIGIAVFPLVVFVAKTGTGPIRWIHRPTLHDVLAFLE
                     HFCGSDHWPLLLLYSAACVAAVAPFSKRLLARDQSWDTWRCQFLLIWLFFPVLLTLLL
                     SLAKPVFYDRYLIFCLPPLTILAAAGLARLRPAWLIAPCLAAFLLLSLQGVFFVYGHD
                     FGDERDAAGAASDFILDHSQPGDGIIFHIPQIRVPYEFFRSVRAGGNLSAAAAIGPEI
                     VYPHSSEEGLDFRDFKARLSADFLGRAVPSHRRVWVVLMYNGPKLPDPTAVLLTKALP
                     AWFPHVQRWQFPKVEVRLYSRE"
     gene            9218..>9621
                     /locus_tag="VEJ00_10935"
     CDS             9218..>9621
                     /locus_tag="VEJ00_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HXX01722.1"
                     /translation="MFRKILIANRGEIAVRVIRACHEMGIAAVAVYSDVDRAALHVRK
                     SDEAYPIGAPAASESYLNIQKILDVAKRSGAEAIHPGYGFLSENARFARACVDAGVKF
                     IGPTAAAMDAMGSKTQARQAMERAGVPIVPGTS"
BASE COUNT         2077 a         2685 c         3062 g         1797 t
ORIGIN      
        1 gtggcggggg cgggatgggg tggctgggca ttgcgccgct catcttcatt tttgcgattt
       61 tttactttct tttgattctg ccccagcagc gccgccagaa gaaatggcag gctatgctgg
      121 accagctcaa aacgggagac aaggtttcca ccagcggggg gctgcggggc acgatcattt
      181 caatgaagga cgacgcggtc cagctgcgca tcccgcccga taatctccgc gtcgaagtaa
      241 ccaaagcctc gatcgtgcac gtgacgacaa ccgaggaaga agggaaaggg aagtgatcac
      301 aggtcaggga tcagggggaa ggggtcagtt gcacttggca gcaagcgacg actctcaact
      361 ggcccctgag ccctggcctc tgacccctga ttttctatga acaagcctct tctctggcga
      421 gcgggaatca tcgttgcaac gctgctcatc ttcctgttcg gaattttcgg cattcctaag
      481 agtttctccc cgcaggggat gatcgcgtcg atgggcgagc acatccacct gggtctcgac
      541 ctgcgcggcg gaacgcacct gattttgcag gtgcaggtca acgacgcggt caatgtggat
      601 gcgcagaacg ccatggaagt tctccgggag caaatgcgca accgcaagat tgactacacc
      661 gacatcaccc agccggatca gcagaataac cccgatcaca ttgtgctgaa aggcgtgcag
      721 gccagttcgc gcacggacct gctgaacatc gtctccgacc ggctgccgga atacgacatt
      781 accgggggcg ctgacaattc ctggaacatg tcgatgaagc cctcgttact gaccgacttg
      841 aaaaacaagg cggtgacaca ggccatcgag accatacgca accgcgtcga ccagctcggc
      901 gtcagcgagc cgcagattga agagcacggg ttggggcagt accagattct ggtgcagttg
      961 ccggacgtgg atgatccggc ccgagtgaag gacatcatcc agaacaccgc gatgcttgaa
     1021 ttcaaacagg gacttggcgg gccctatccg agcgagcagg cggcgctgca ggcgcaacca
     1081 accccgggtc ttctgccgcc cgatgcaatt ctcgttcccg ggcacagcat gccgggaacc
     1141 ggcgaagaag ggccggcctg gtatctggtg tctcgcgtct cggcggtgac cggaaaggac
     1201 ttgcgggatg cgcagcccag ccgcgatccg aatggccagc ccagtgtcag cttcacgctg
     1261 acaggcgacg gcgggcagcg tttttacaac ttcaccagcg cgcacgttgg cgaccctctg
     1321 gcggtggtgc tcgacaacaa ggtccaggaa tgggcgaaca tcaaggagcc gattcgcgat
     1381 cgcggcgaga tcagcggcgg gcgcatgagc gagcagcagt cgaaggatct gtcgatggtc
     1441 ctgcgctcgg gcgcgctgcc ggcgggcata cggtatctgg aagagcgcac ggtgggaccg
     1501 tcgctgggcg cggattcgat tcgcgccggc gtgcgcgccg cgattgcggg catgctcgcg
     1561 gtgctgatct ttatgctgat ttattaccgc ggggccggga tcaacgctga cgtggccctg
     1621 attctgaatc tcattatcct gctaggattc atgggctact ttggcgcagt gttgacgctg
     1681 ccgggaattg ccggcgtcat tctcaccgtc ggtatgggtg tcgattccaa tgtgctgatc
     1741 ttcgagcgta tccgcgagga gttgcgcaac ggcaagacgc cgccttcggc ggtagatcag
     1801 ggtttcagcc atgcctgggt cacgatcgtt gatacgcacg tcaccaccat cgtctcggcg
     1861 gcgattctgt tcttgtttgg aaccggcccg gtgaaaggtt ttgcagtgac gctgacgttt
     1921 ggtttgctgg cgaatctgtt tacggcggtg ttcgtatcgc gcgcgatctt cgactggatt
     1981 ctcagccgca agcagcgtgg agaagcgtta agtatttgaa gagtcgcgag tccggagtcc
     2041 cgagttgcga gcaaaacctg gtgagtatgt cagactcggg actcgcgact cacgactcgg
     2101 ggctgcgaag ctaagtcata gataaataag gggctaggca aaggtggagt tctttcacga
     2161 cgtcaatata gattttctgg ggaaaaagtg gtattttctc gccttttccc tgatctttag
     2221 cgttgcgggc gtgctttcta tgttgttctg gcacggcatc ccgtggggtg tcgacttccg
     2281 cggcggcacg ttggtgtacg tgaagttctc gcatacgccg gatttgggtg tcgtacgcac
     2341 actgatggac cgcgcgggct tgcgcgatcc caagattcag acctatgggc cgccttcgaa
     2401 caacgaggtg ctgatcgacc tttcccagaa agaaacgggg gaacaggccc tcggacaggg
     2461 caagacgcaa atcattaatg cgctggagac caacgctccg gcgggcaaac agtgtagcgc
     2521 cgtcggcaac agcgtcggcg catgcgatct gaacaatgcc agctctttga caatcaagaa
     2581 ctatctgatg gataaagatc cgctccacct gggcaccgat gccgaccaga agtacacggc
     2641 acaggctcgg gacatcgtcg actaccgcga caaggtccag ggcggggtgt tgagctcgat
     2701 cgaccaattg aaggccagtt cgaccgatcc tgccgtggtt gccacgttag gtgacggctt
     2761 ctttctctcc gattttggcg ttcgcaacgt agaaatcgtt ggaccgacgg ttggagcgca
     2821 actgcagaag caggcggcgc tggccacgat ctattcgttg gctggaatgc tggtttacct
     2881 gggtttccgg tttgagtgga tttacggcgt tgcggctgta gtgaccgtgt ttcacgatac
     2941 attgatcacg gtaggggcgt tttccctgac gaaaaccgac atttccctaa ctgtgattgc
     3001 ggccattctg acgctgattg gttactccaa taacgacacg atcgtggtat ttgaccggat
     3061 ccgcgaaaat attaaactca tgcggcgcga gaagcttgcc gacgttgtca atcggagcat
     3121 taatcagacg ttgagcagaa cgatcttgac agcgggcctt accttcctta ccgtactcgc
     3181 actctttttg ttcgggggcg aggtgcttca tggtttcagc ctcgcgcttg taataggcat
     3241 cttaatcggg acgtattcgt cgatcgcgat cgcagcgccg attctggtgg cgtatcaaga
     3301 ttggcggatc aaccggggaa agcgtcccgt ggccatgccg gcggccaagg gcaggtaagg
     3361 ggaatacgca ttgggtcgct ggttgaccgt tatgttaggc cttcatacgt aaggacttag
     3421 aagggacgct gtggtgcgga aattgacgct gcggcgggca aggatcccgg gagcaaacca
     3481 gacgcgcccg gtgtctaagg ttccgtaggg tctgagtagg ggggaagctt atgtttgaag
     3541 acagcctgat tgagtccggg ggaaggctaa gaaccaagcg tgggcggacg tcggccatcg
     3601 cgttctttgt gcagatctgc atcatcgtcg tgatgatcct gatcccgcta atcttcacgg
     3661 aagcgttgcc gagaacgcag ctcatgacgt tcctggtagc accgccgcca cccccgccgc
     3721 caccgcctcc ggcagctgcg ccggtaaaga tcgtcaaggt catccagacc gacattgtca
     3781 atggccagct gcgcacgccg accaagattc cacagaaagt acagatgatc aaagaggacg
     3841 aggcgcctcc gccggtgatg gctgcgaccg gagtggtcgg cggcgtcccg ggtggagttc
     3901 cgggcggatc gatgggcggc gtgattggca gcgtgctcag ctctacgccg gtagcggtgc
     3961 cgaagatcgc gactccgcag cgcgtgcgcg tgtcgtcggg cgtgtcgcag ggactgctgg
     4021 ttcgtcgggt aaatccgacg tatccgccgc tggcgcgcca ggcccgcatt cagggcgtgg
     4081 tgatcctgca ggcgcagatc agcaaggcgg gcgatatcga gaacctgcag ttgatcagcg
     4141 ggcatccgat gctggcgccg gccgcgatcg aggccgtgaa acagtggaag tacaaaccgt
     4201 acctgctgaa cggcgagccg gttgaggtcg acacccaggt gcaggtcaac ttcacgctct
     4261 caggaggtta aactcccgag cgccgggtga attggaattc gtagtggaag tgtgcgatct
     4321 aacctcccct ctcaccaaga acgcggtggc ggggaggagc tttccaaaag ctcgggcagg
     4381 gccgccatat cgcgaattcc tcgtcagata tggttggccg ggtccgggct tctgcagcgt
     4441 aggctggtgc agtttccggg ttggcgctgg caccgccgga cgcatcacag tgtctaaagt
     4501 gagagcaaaa ggtttccgag gagggaaaag caactcatgt tcgcaactta cgtagcagga
     4561 gctattctca gtcacgtacc cgtcgccgca tcctgggtta tgcaggaagg cggcacgctg
     4621 gcgaccgatc cccttagcct gtggaaggcc atgggatggg tggcgagaac cgtcgttatc
     4681 attctgttca tcatgtcggg ctggtcgatc ggcgtgatga tcgaccgctg gatggcttac
     4741 agcgcggccc gcaaacaatc gcgtgctttt gccccggccg tagcaggcgc cctgagggat
     4801 ggccgcatcg atgaggccat caaggttgcc gaacgcaaca agaagagcca cctggccaag
     4861 gtcgtcacgg cgggcctgat ggaattcaaa gctcaccagg acagccccgg cgccattccg
     4921 ggcgagacga tcgaggcctc gaagcgcgct ctggagcgca ccgaagccat cgttcacgcc
     4981 gaattgaagc gcggcctggg cggcctcgcc actatcggct cgacggctcc cttcgtgggg
     5041 ctgttcggca cggtgatggg cattctgaac gccttcatcg gcatcaacac atcgaaggcc
     5101 accggtctgg cggcggttgc aggcggcatt gcggaagcgc tggtgacgac agccatcggg
     5161 ttgctggtcg ccatcccggc cgtgatgatg ttcaactatc tgactggccg tgtggaagcg
     5221 ttcgacgtgg aaatggacaa ctcgtcgagc gaattgatcg actacttcct gaagaagcgc
     5281 ggcgacgttc gccggtagag tcccccactt ctcgcgcaaa aagcgtgcga gatggggcac
     5341 ccagccgcag tgagttgctg agccggcgct ggaatgctcc agcgccggac cctcccgcct
     5401 tggcggcagc cacaacggga gcgtagtgga agcacgggag acagccagct atgtcaatgg
     5461 caaagcgaga cgaaggcaag aagatcaact ccgacatcaa cgttaccccg atggtggacg
     5521 tgatgctggt gctgctgatc atcttcatgg tggtcacgcc catgttgcag aaaggtgtca
     5581 gcgtggacat ggccaaggtg aacaatccca cgccgatgga ggacgccgac aaggaagacg
     5641 ccctgctcgt ttctgtgacg cgggacggga aaatctactt tggcagcgac ctgattacga
     5701 ttgacggcct cacgacgaaa gtgaaggacc gcctggccaa caagcccgac aagcgggtgt
     5761 atgtgaaagc cgatatgaga gcgcgctttg gcggcgttgt cgaagtggtg gacgctgtgc
     5821 gcgcggccgg ggtcgacgat ctgggcttgc taaccgaaca gaagaagact gctccaggcg
     5881 ctccgcccgc ggcggctgcg ccaggacaat agaggagaac tgccatggga atgggagtta
     5941 gcgcaggcgg aggccagagc gccaacatca acgtaacacc gctcatcgac gtgttgctgg
     6001 tgctgctgat catcttcatg gtgatttcgc cggtgacgcc gaagggcctg gatgcgctgg
     6061 tgcctcagcc gccgccaccg aacgcgccca agaacaccac gcccgaccgc accatcgtcg
     6121 tgcagctgat cgaccgcggc gccggcaccg agcccggcct gaaggtcaac caggaggacg
     6181 cgacctggga caatctgcag gggcggctgg aagacatcta caagcagcgc gcggaaaagg
     6241 tgatgttcgt gaagggcgat gactcgatcc ccttcggcaa cgtcgccaat gtgatcgata
     6301 ttgcccacgc ggccggtgtg gacaaggtcg gattgatcac cgcgaagatc gaggcgggca
     6361 actagaaggc gcgctccgcg cgagtgtcag ttcccgccct ggccgcagag agcgcggctg
     6421 ggatgggggc acccgcagta gaatttgccc gtccagccca cgcgcttgtc aaaccgatgt
     6481 ctctgggcct aggatgctgt ggcgagggag actaataccg caatgaataa gaacctgagg
     6541 ctgctcacgc tggcggcggt agcgctggcg cttttctcct ctgtgggctg caacaagcta
     6601 cgggcccgcg accagctcaa caagggtgtg gaagcttaca agaattccca ttacgagcag
     6661 gccatcgatc acttccagca ggccgtgcag ctcgacccga gcctcatcaa tgctcgcatg
     6721 tatctggcca ccgcatttgt atcccagtac attcccggcg tggactctcc cgacaacctg
     6781 cgcactgcgc agcaggctat tgacgaatac cagaaggtga tcgacgccaa tccttcccgc
     6841 gatcagaagg tcaacagcgc caaaggcatc gcgtacctgt acctgaacat gaagaggtgg
     6901 gacgatgcca agaagtatta ccgcatggcg tcagatcttg atccgaacga tcccgaacct
     6961 tactactccg taggcgtgat cgattggacc gcctgctatc agccgcgcat ggaagagcgt
     7021 gccaagctgg gcatgaagcc ggaagagcat ctgaacccca agaacaaaga tcagaagaaa
     7081 gcttgcgacg agctgaagac aaagaacgcc cccgctatcc aggagggcat cgacagcctg
     7141 aacaaggcaa tccagcttcg gccggattat gacgacgcta tggcctacat gaacctcatg
     7201 taccgcgaga gggcagacgt cgaatgcgac gatctctcgg cgcgcgaaga agatctaaaa
     7261 gcggccgatc actgggtgga cgaaaccctg cgtgtgaaga aagccaaggc cgagaaggaa
     7321 gcagaaaagg ccggcggtgg aatcacgatg gaccaatcga aatagatcct acggcggcag
     7381 gcatcgggcc tccttcgctt ggaagcgaag gaggcttttg cattttgtga tcgacgcctg
     7441 gcccgactaa gcgagccttc cagacctatt ctctcgaata aaggcggacc tcgaccttcg
     7501 ggaattgcca gcgctgcacg tggggaaacc acgccggaag agctttcgtt aacagcacag
     7561 ccgtgggatc aggcaacttg ggcccgttgt acatgagcac gacccaaacg cggcgatggc
     7621 tgggtactgc cctgcccaga aagtccgccg aaagccgtgc cttgaaatcg cggaagtcaa
     7681 ggccctcctc tgaagagtgg ggatagacga tttccgggcc gatcgcagcc gcggcggaaa
     7741 gatttcctcc ggcccgcacg gagcgaaaga attcgtaggg gacgcggatc tgcgggatgt
     7801 ggaagatgat gccgtcgccc ggctgggagt gatcgagaat aaagtcgctg gctgcgccag
     7861 cggcatcgcg ctcatcgccg aaatcgtggc cgtagacgaa gaagacaccc tgcagagata
     7921 gcagaaggaa cgccgcgagg catggggcaa tcagccaggc cgggcgcagc ctcgccagtc
     7981 cagccgctgc cagaatcgtc agcggaggca agcagaagat caggtagcgg tcatagaaga
     8041 ctggcttggc cagtgagagt aatagcgtca acagcacagg aaagaagagc cagatgagca
     8101 agaactggca tcgccaggta tcccagcttt gatcgcgggc cagcagtcgc ttgctgaacg
     8161 gagccacggc cgccacgcag gctgcggagt agagcaaaag gagcggccaa tgatcgcttc
     8221 cgcagaagtg ctcgaggaac gcaagcacgt cgtgcaaggt cgggcgatgg atccagcgga
     8281 tgggcccggt tccggtcttg gccacaaaaa ccaccagcgg gaacactgcg attccgatga
     8341 ccttccaagc acgtttgagc tctgtcttgc gtgattcgtt cgcattgcct ggaggcaggc
     8401 ccagtcgcca cacggtcagc cactgcgcca cgatcagcag tagcgcatag agatgggcgt
     8461 aagcggctag gacgctggtc aacatgtact taaacccgag gggccgacgt ggttcgcgaa
     8521 ctgcggcaac caggaagccc gaggaaagcg tcgccagcaa aacgagcagg gcatagctgc
     8581 gggcttcctg tgcgtagcga acgtggaagg cgttgatggc aaacagagcg gttgcgatga
     8641 gggcgaccag gcggccattg tctcgctccg agaagagaag cccggccagc cagtaaatgg
     8701 ccggcagcgt ggctgcggcg atgagcgccg agaggctccg gatgaagaac gcgctccagc
     8761 caaagtgaag ccagaggcgc aggaggccgt agtacagtgc catattggct tcgcgccacc
     8821 acaacacgtg aagaaaactt ggccacttca tgcgcgtgac ttccacgctg aagcactcgt
     8881 cgaaccagaa gggtttgcag gcgaggcagg caaatcgaaa cgcaagagcg gcggcgaaaa
     8941 gcagtagcag cgtggcccag tgggcttgcc gcaccccgct agatcggact cggtgggttg
     9001 cgccgccgtt gggcgacacg tcagaccgca tcacgctgac tatttgagga tctgccaatt
     9061 ttcagatcat actatgccgg aaaacaggca gaagtttgct tgcgtggtcg ccggttgatt
     9121 gcgggttttt cctccccgca cggcgccaga cgtcgaggtc cagtacaata tccgctccag
     9181 agcgcgccgg aagagctgac attccggctc agttcccatg ttcaggaaga tcctcatagc
     9241 gaatcgcggc gagattgcag ttcgggtgat ccgcgcctgt cacgagatgg gaatcgctgc
     9301 ggttgcggtc tactccgatg tggaccgggc tgctttgcac gtgcgcaagt ccgacgaagc
     9361 gtacccaatc ggcgcaccgg ctgcctccga gtcgtatctg aatatccaga agatccttga
     9421 tgtggcaaag cggtcgggag cggaggccat ccatcccggc tacggttttc tttcagagaa
     9481 tgccaggttc gcccgcgcct gcgtggatgc aggggtgaag ttcattggcc cgaccgcggc
     9541 ggcgatggat gcgatgggat ccaagactca ggcccgccag gcgatggagc gagccggagt
     9601 tcccattgtg cccggtacct c
//
LOCUS       DASPUL010000458         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1585080, whole genome shotgun sequence.
ACCESSION   DASPUL010000458 DASPUL010000000
VERSION     DASPUL010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585080"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="VEJ00_10940"
     CDS             <1..1273
                     /locus_tag="VEJ00_10940"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HXX01723.1"
                     /translation="ARRMNTRNGTVKTGIDVLEEHGFDILQAKSPADESRAGSGDQQV
                     VDTTKPEVTTPEATPGVTKRHIGLVTNQTGIDIEGRRTIDVLAQAPGISLDAIFSPEH
                     GVTGALDTTHVDNTTDAVTGIPVYSVYGGTDAERRPSVDVLKTLDAVVFDIQDAGARF
                     YTYETTLGYFLEAAAQAGIELIVLDRPNPVTGAFVQGPVSDAGQESFTNYWTVPPRHG
                     MTVGELAKMFNAERNINARLTVVPMEGWERGDWFDSTGLLWVNPSPNLRSVYEASIYP
                     GVALVEGTNVSVGRGTDSPFELVGAPWIKSRELAAYLNARAIAGVRFVPVTFSPTASA
                     YSGQECQGVNIVVTDRNGFDAPELGIELAAALHKLYAADFKLEKIQGLLVNQSVYEAL
                     AEGEDPRRIAQDWQEGLEKFEKVREKYLIYK"
     gene            complement(1298..1984)
                     /locus_tag="VEJ00_10945"
     CDS             complement(1298..1984)
                     /locus_tag="VEJ00_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01724.1"
                     /translation="MREGLLEIFQETYSELRPGFSLPELKIDFFAFANVNNTIRLRNG
                     RLLVRLSDLLEGAPETVLRAIAHILLAKMYRHPIDRGHAAQYRRYVASHEIVRKVHLV
                     RQMRGRKRLRSPSGHFYDLDAIFDELNSRFFHGLMARPRMSWSQTKTRRILGHYDPAH
                     NAIVISRIFDHFAVPRYVLEYIVYHEMLHLKHPVKLRGSRRCVHSAEFQAEEKLFPRV
                     DEANSFLKRL"
     gene            complement(2079..2639)
                     /locus_tag="VEJ00_10950"
     CDS             complement(2079..2639)
                     /locus_tag="VEJ00_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01725.1"
                     /translation="MHHSTIVLTLLLAAGPISLAQRHRDPLTQPEIDQIRDTSWEPRE
                     RLKLYVEFARARLVKLEQMRSDPKTKDRATQTHDLLDDFQLLYDELNDNIDTYVDRKD
                     DIRKPLKLIIEADTEFQAKLRALKDAADVPAAETSTYEFVLTNALDTVDTSAEEHRKL
                     LADQEDAAKHKKLNSSSNTKTAGKPE"
BASE COUNT          535 a          755 c          924 g          545 t
ORIGIN      
        1 ggcgcggcgc atgaacacgc gcaacgggac ggtcaagact gggattgatg tgctcgaaga
       61 gcacgggttc gacatactgc aggccaaatc cccagccgac gaaagccgcg ccgggtcggg
      121 cgaccagcag gttgttgata caacaaagcc ggaggtgacg actccggaag ctacgccggg
      181 ggtgacgaag cgacacatcg ggctcgtgac gaatcagact ggaatcgaca ttgaggggcg
      241 gcggacgatc gacgtgctgg cgcaggctcc ggggatttcg ctcgatgcga ttttcagtcc
      301 ggagcacggg gtgaccggag cgctcgacac cacgcacgtt gacaatacga ccgacgcggt
      361 gacgggcatt cccgtttaca gcgtgtatgg gggaacggac gcggagcggc ggccgtcggt
      421 ggacgtgctc aagactctgg atgccgtggt gttcgacatt caagatgcgg gggcgcggtt
      481 ttatacgtac gaaaccacgc tgggctactt tctcgaagcg gcggcacagg ccgggatcga
      541 gttgatcgtg ctcgaccggc cgaatccggt cacgggagcg ttcgtgcagg gccctgtttc
      601 cgatgcgggc caagagagtt tcacgaacta ctggactgtg ccgccacgcc acggcatgac
      661 tgttggcgaa ctggcgaaga tgttcaacgc cgagcgcaac atcaacgcca ggctgaccgt
      721 agtgccgatg gaagggtggg agcgcggcga ctggttcgac tcgaccggat tgttgtgggt
      781 gaatccgtcg ccgaacttgc gcagcgtgta cgaggcgtcc atctaccccg gggtagcgct
      841 ggtcgagggg acgaacgtct cggtgggccg cggtacggac tctccgttcg agctggtcgg
      901 cgcgccatgg atcaagagca gggaactggc ggcttacctg aacgcgcgcg ccatcgccgg
      961 ggtgcgcttc gttccggtga cattttcgcc gactgcgagc gcgtacagcg ggcaggagtg
     1021 ccagggtgtg aacatcgtcg tgacggatcg gaatggattc gacgcgccgg aactcgggat
     1081 cgagctggcg gcggcgctgc acaagctcta tgccgcagat ttcaagctcg agaagatcca
     1141 gggactcctg gtcaatcaga gcgtatatga ggcacttgca gaaggcgagg atccgcggcg
     1201 cattgcgcaa gactggcagg aaggcctgga gaagttcgag aaggtccggg agaagtattt
     1261 gatctacaag taaggcctgc atcgtcgcgg atgtgattca cagccgcttt agaaagctat
     1321 tcgcctcgtc aacccgcggg aacaattttt cctccgcctg aaactccgcc gagtggacgc
     1381 agcgccggct gccgcgcagt ttcacgggat gcttaagatg cagcatctcg tgatacacga
     1441 tgtactccag cacatagcga ggcacggcga agtggtcaaa gatgcggctg atgacgattg
     1501 cattgtgcgc cggatcgtag tgaccgagaa tccggcgcgt cttggtctgg ctccagctca
     1561 tgcgcgggcg ggccatcagc ccatggaaga agcgggagtt caactcgtcg aagatcgcat
     1621 ccaggtcgta gaagtgcccg cttggtgaac gcaggcgttt gcggccacgc atctggcgaa
     1681 ccaggtgcac cttgcgcacg atctcgtgac tggcgacata cctccgatac tgcgccgcat
     1741 gcccgcgatc gatcgggtgc cggtacatct tggccagcag gatatgggca atcgcgcgta
     1801 gaacggtctc cggcgcgcct tccagcagat cagacagccg taccagcagt cgcccgttgc
     1861 gcaaacggat cgtgttgttg acgtttgcga aggcgaaaaa atcgattttc agctcgggca
     1921 gggaaaagcc gggccgcagt tcgctataag tttcctggaa gatctcaagc aggccttcac
     1981 gcacggtgag gctagggtag cagcggtgag tggtcagtgg ccagtggtcc gtagtgacag
     2041 gagaaccata ggcgtcacaa gcgacaatcg gccactgatc actccggttt tccggcggtt
     2101 ttggtgttcg aactcgagtt cagcttcttg tgcttggccg catcttcctg gtccgccagc
     2161 agtttgcgat gctcctcggc tgatgtgtct acggtatcga gggcattggt cagcacgaat
     2221 tcgtaggtgc tcgtttccgc cgctggcacg tccgcggcat cctttagcgc ccgcagtttc
     2281 gcctggaact cggtgtcggc ctcgatgatc agcttgagcg gcttgcggat atcgtccttg
     2341 cggtcgacgt aggtgtcgat gttgtcgttg agttcatcat agaggagctg gaaatcgtcg
     2401 agcaggtcgt gcgtctgggt ggcgcgatcc ttagtcttgg gatcgctgcg catctgctcg
     2461 agcttcacca gccgtgctcg ggcaaattcg acatagagct tcaatcgctc gcgcggctcc
     2521 cagctggtgt ctcgaatctg gtcgatctcc ggttgcgtaa gggggtcgcg atgacgctgt
     2581 gcgagggaaa tggggccggc cgccaacagc agcgtcagga ctatcgtcga gtggtgcatc
     2641 agcgatctca tttggtgcct ttcggaaaca ttgcggccag cagatcgtcg cgcacgcgct
     2701 gcaaccgttc gatagcgtcg tgcaccgggg ggcggtcgtc atgcggcagt ccgtgcata
//
LOCUS       DASPUL010000459         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1181132, whole genome shotgun sequence.
ACCESSION   DASPUL010000459 DASPUL010000000
VERSION     DASPUL010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181132"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VEJ00_10955"
     CDS             <1..718
                     /locus_tag="VEJ00_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01726.1"
                     /translation="AKWPVNRPHRNPEWYEFKKHFANYIGLRKKLWGISAGEKMWGFF
                     YHRTAKNMGGITHHLAPQTELAELAHPFYNQFARGGEGHLEVGKNVYYTVHKLCHMVL
                     ALKPFGCMPSSQSDGVQSAVINKFKDMIFLPIETSGEGEVNAHSRVQMALGEAKVKAK
                     AEFEECLKSTGKSMEEVREYIDEHPELKRPFYHVPHKEGVAGTAAQFILHVGDRLDKD
                     TRFWKRSRVRVPEVAAASGD"
     gene            821..>3246
                     /locus_tag="VEJ00_10960"
     CDS             821..>3246
                     /locus_tag="VEJ00_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HXX01727.1"
                     /translation="MHDHKGPALIQITPLEPAVSIQQTKHAHQPHDHSTKFFVGLDVG
                     STTVKAVVVDAASDQVLWQDYQRHETKQPEKTLEFLRRIEAEVGVNRHNTRMFLTGSG
                     GTGIAEQIGAKFVQEVTAVSLAVEKLHPEVYSVIELGGQDAKIIVFKDDEETGRKKKI
                     PSMNDKCAGGTGAVIDKINAKLKIPTEELASQKYHGIKLHKVAGKCGVFAETDINGLQ
                     KVGTPPDELMASLFEAIVLQNLSVLTRGHTLRPHVLLLGGPNSFIQGMREAWQANIPR
                     MWQERKVQIPNGAKPEDLIKVPQNAQYFAALGAVEFGRGEDDCVGCYCGHEKLVHYIE
                     YGRQEEKAKSGGKGLVEKPEELDVFKEAYRRKKFTPAVFAKNSTVGGFIGIDGGSTST
                     KAVLLSESGDILCKSYQLSNGNPIQDTIEMFENLRGQVESQNAKLEVLGVATTGYAKD
                     ILKDVLKADVALVETVAHTESALKFYEDPHVIVDVGGQDIKIIILHNGRVKDFKLNTQ
                     CSAGNGYFLQSTAEGFGLSVNQYADLAFSAQAMPVFGYGCAVFMQSDIVNFQRQGWRA
                     EEILAGLAAVLPKNVFLYVASIPNLAALGSRFVLQGGTQNNLAVVKAEVDFIRNSFRA
                     TGKQPEIIVHEHCGESGAIGAAQESLRLWRNGQTTTFVGLDAVRKIEYRTTRNEDTRC
                     HFCKNDCLRTFIDIRTQPKNDLSFVPIQKVTKVPLMHGEQRLIIATCEKGTVEDVNDM
                     KEIKAGLDSIRAQHPNFVDYASKEVFRPTNAKSVADPLPTKGWAGWRKGDKERCALME
                     NRKKVRIGIPR"
BASE COUNT          788 a          917 c          951 g          590 t
ORIGIN      
        1 ggcgaagtgg cccgtcaacc gcccgcaccg caatccggag tggtacgaat tcaagaaaca
       61 cttcgctaac tacattggcc tgcgtaagaa attgtgggga atctctgccg gcgagaagat
      121 gtggggcttc ttctaccacc ggacagcaaa gaatatgggg ggaattaccc atcatctcgc
      181 gccgcagacc gaactggccg agctcgcaca tcccttctac aaccagtttg cgcgcggcgg
      241 cgaaggacac ctggaggtcg gcaagaacgt ttattacacc gtgcacaaac tttgccacat
      301 ggtgctcgcg ctgaagccgt tcgggtgcat gccgtcgtcg caatccgatg gcgtgcagtc
      361 agccgtcatc aacaaattca aggacatgat cttcctgccc atcgaaactt ccggtgaggg
      421 cgaagtcaat gcgcacagcc gtgtgcagat ggcactcggc gaagccaaag tgaaggctaa
      481 agcggagttt gaggagtgcc tgaagtcgac aggcaagagc atggaggaag tccgcgaata
      541 catcgacgag catcccgaac tcaagcggcc gttctatcac gtgccccata aggagggagt
      601 cgcgggcact gcggcacagt tcatcctgca cgtcggagac cggctcgaca aggatacgcg
      661 attctggaaa cggtcgcgcg tccgcgttcc cgaggtggcc gccgcgtccg gcgattaaat
      721 cattgaccgc cgagcacgcc ggggacgcgg agagaatccg ttcttcggcc tactcggcga
      781 tctcggcggt taagcctttt gaccttcgaa agaggagttc atgcacgatc acaaaggccc
      841 tgcacttatc cagatcacac cgcttgagcc tgcggtttcg attcagcaga cgaagcatgc
      901 ccaccagccg catgatcaca gcacgaagtt ttttgttggg ctcgacgtag gttctaccac
      961 ggtcaaggcg gtagtcgtcg acgctgccag cgaccaggtg ctgtggcagg actaccagcg
     1021 ccacgaaacc aagcaaccgg agaagacgct tgagtttctg aggcgaattg aagcggaagt
     1081 cggcgtcaat cggcacaaca cgcgcatgtt tctgactggt tccggcggga cgggcatcgc
     1141 cgagcagatc ggcgcgaagt tcgttcagga agtcaccgcc gtgtcgctgg ccgtcgagaa
     1201 gcttcatccc gaagtgtatt cggtgatcga actcggagga caggacgcca agatcatcgt
     1261 cttcaaagac gatgaagaga ccgggcgcaa aaagaaaatt ccttcgatga acgacaagtg
     1321 cgcgggcggg accggcgcgg tcatcgacaa gatcaatgcc aagctcaaga ttcctaccga
     1381 ggaactcgcg agccagaagt atcacggaat caaactgcac aaagtcgctg gcaaatgcgg
     1441 ggttttcgcc gaaactgaca tcaacggatt gcagaaggtc ggtactcctc ccgatgaact
     1501 catggcatcg ctgtttgaag ccatcgtgct gcaaaacctc agcgtcctga cgcgcggaca
     1561 caccctgcgg ccgcatgttc tgctgctggg cggccccaac agtttcatcc agggcatgcg
     1621 cgaagcctgg caagcaaaca tccctcgcat gtggcaggaa cgcaaagtcc agatcccgaa
     1681 cggggcgaag ccggaggatc tcatcaaggt tccgcagaac gcgcagtatt tcgcggcgct
     1741 cggagcggtt gagttcggcc gcggcgagga cgattgcgta ggttgttatt gcggtcacga
     1801 aaagcttgtt cactacatcg aatacggccg gcaggaagag aaagccaaat ccggcggcaa
     1861 gggattggtc gaaaagccgg aagagcttga cgttttcaag gaagcataca ggcgcaagaa
     1921 attcacgccc gcagtattcg ccaagaacag caccgtgggc ggattcatcg gcattgacgg
     1981 cggatctaca tccaccaaag cagttctctt gagcgagagc ggcgacattc tctgcaaatc
     2041 gtaccaactc tcaaacggta atccgattca ggacacaatc gagatgttcg agaacctgcg
     2101 cggccaggtc gagtcgcaga acgccaagct ggaagtgctg ggcgtcgcaa cgaccgggta
     2161 tgcgaaagat attctgaaag acgttctcaa agccgacgtg gccctggtgg aaactgtcgc
     2221 gcacacggag tcggcgctga agttttacga agatccgcac gtgatcgtgg atgtcggcgg
     2281 ccaggatatc aagatcatca tcctgcacaa cggccgtgtg aaagatttca agttgaacac
     2341 gcaatgctcc gcggggaacg gatacttctt gcagtccaca gcggaaggct ttgggcttag
     2401 tgtcaatcag tacgccgacc tcgcgttttc cgcgcaggcg atgccggtct tcggttacgg
     2461 ctgcgctgtt ttcatgcagt ccgacattgt gaatttccag cggcagggtt ggcgcgccga
     2521 ggaaatcctc gccggcctcg cggccgtgtt gccgaagaat gtttttctgt acgtggccag
     2581 cattccgaat ctggcagcac tcggttcccg cttcgtgctg cagggtggaa cgcaaaacaa
     2641 tcttgctgtc gtcaaggcgg aagtcgactt catccgcaac agcttccgcg caaccggcaa
     2701 gcagccggaa atcattgtgc acgagcactg cggagagtcc ggagcgatcg gcgccgcgca
     2761 ggaatcactg cgcctgtggc ggaacggcca aacaacaaca ttcgtcggcc tcgacgctgt
     2821 gcgcaaaatc gaataccgca ccacgcgcaa cgaagatacg cgttgccatt tctgcaagaa
     2881 tgactgcctg cgcacgttca tcgacattcg cacgcaaccc aaaaacgacc tcagcttcgt
     2941 gcccatccag aaagtcacca aagtcccgct gatgcacggc gagcagcggt tgatcatcgc
     3001 cacctgcgaa aaaggcacgg tggaagatgt gaacgacatg aaggaaatca aggccgggct
     3061 cgattccatc cgcgcccagc atccgaattt cgtggactat gcctcaaaag aagtcttccg
     3121 ccccacgaac gcgaagagcg tagctgatcc cctgccgacg aaaggatggg ccggctggag
     3181 aaagggcgac aaagagcgct gcgcgctgat ggagaaccgc aagaaggtcc gcatcggcat
     3241 tccgcg
//
LOCUS       DASPUL010000460         3543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1453200, whole genome shotgun sequence.
ACCESSION   DASPUL010000460 DASPUL010000000
VERSION     DASPUL010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453200"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VEJ00_10965"
     CDS             <1..835
                     /locus_tag="VEJ00_10965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXX01728.1"
                     /translation="PALDRLRAGGIEVKVAITKSSGEAAEIARAAYGRGVRKFIAVGG
                     DGTAYEVVNGLFPQPVNGEQPTLAFLPLGTGNSFLRDFSDRGVEHAIEAMLAGRSRPV
                     DVIRLRHREGVVHYINLLSMGFSADVATLRARRFGGWGELGYVASIFLTLARFNRRPF
                     PVRIEGATAFDSRRCLFLTFSNSKFTGGTMMIAPDAQVDDGLVEYVRWGPIGRLGLIR
                     NLPTLYDGTHVKHPMAERQAVRRVEFNLDRPVDVMVDGEVLSLQCEELDVLPGALNVV
                     V"
     gene            860..1285
                     /locus_tag="VEJ00_10970"
     CDS             860..1285
                     /locus_tag="VEJ00_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01729.1"
                     /translation="MSEERRKRQQERSSAGQGSEKTRKYLTWGGVLILFLGAFGAGRY
                     YKNHRYDAFARCLASKQVKMYGLYWCPHCADQKAMFGASFRYVPYVECAIKGSKEMAP
                     ECKIAGAKLFPSWQFGTNPPKEGVLSLEELSDKTGCRLP"
     gene            1270..1755
                     /locus_tag="VEJ00_10975"
     CDS             1270..1755
                     /locus_tag="VEJ00_10975"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HXX01730.1"
                     /translation="MPSAVNDEGQTSLPGSCLSRSRSILFSAIAVLSLAGAIVSAISL
                     QRHYAKSETAFCQFGQKFNCDIVNRSEYSSVEGIPVAAIGVAGYAALFLLSTFWRLRA
                     DTPNRLLAASIAGLAFALYLTYIEAYVLTTWCILCVSSQLLILLISLLAALVKFRDVK
                     T"
     gene            1795..2679
                     /locus_tag="VEJ00_10980"
     CDS             1795..2679
                     /locus_tag="VEJ00_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXX01731.1"
                     /translation="MEEIQQTENPAAGIAPAFIRAESATGDLRLHPFTSRIFRNTRFL
                     RVWLPPGYDDSDNITRRYPVLYLNDGQNLFEPSTSFTGVEWGVDETADRLIRGGVIPP
                     LIIVGLDNAAKDRIREYMPHRSLHPMMLRVQGSRYPNLLIKEVMPFVLRNYRVAAGPE
                     NTGLGGSSLGALIALYTASVRPGLIGRLLLESPSLWASNRQSIKESRHVRRWPDRVYL
                     GTGTSEVGRPERDQSVVDDVRELAGILRRAGLDDRRLKLVIEEGAPHHESAWARRFPE
                     ALKFLFANEAAAPQVPKG"
     gene            complement(2726..>3543)
                     /locus_tag="VEJ00_10985"
     CDS             complement(2726..>3543)
                     /locus_tag="VEJ00_10985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HXX01732.1"
                     /translation="CLWRACPRLREYGALTALAAIAFPGFFHLIAWGQTSALALACFT
                     VMFFQLRDGRCFLAGLALGCLIFKPQLGLAAAVVLLAAGAWRTVLGAGLSAAAQLAIG
                     VLYYGLEPMRRWLHMMLHVRAVLPLLEPRPYQTHCLRTFWSMLVPWPSLSFSLYILSA
                     IAVLGMTVACWKRPGVPLALRYASLLLATVLVAPHLTVYDLVILAPAFLLLADWLLGQ
                     PRSSAIGWLGILLYLIYMLPLLGPFARLTHVQLSVLAMMACSYMADRLGRQTA"
BASE COUNT          706 a         1093 c         1076 g          668 t
ORIGIN      
        1 tccggcactc gatcgcttgc gggctggagg catagaggtc aaggtagcca tcacgaagtc
       61 aagcggcgag gccgccgaaa tcgcgcgcgc cgcctatggg cgcggcgtcc gcaagttcat
      121 cgccgtcggg ggcgacggca ccgcctacga ggtcgtgaac ggattgtttc cccagcctgt
      181 gaacggcgaa cagcctaccc tcgcatttct gcccctgggc accggcaatt cgtttctgcg
      241 cgacttcagc gatcgcggcg tcgaacatgc catcgaggcc atgcttgccg gacgcagccg
      301 ccctgtcgac gtgattcgcc tgcgccaccg ggaaggggtt gtgcactata tcaatctgct
      361 gagcatgggc ttttctgccg acgtcgcgac actgcgggct cgccgcttcg gcggttgggg
      421 cgaactcgga tacgtcgctt ccattttcct tacgctcgct cgctttaacc gccgaccgtt
      481 tccggttcgc atcgaaggcg cgaccgcatt tgacagccgg cgctgcctgt ttctcacctt
      541 cagcaacagc aaattcaccg gcggcaccat gatgattgct cctgacgcgc aggtcgacga
      601 tggcctggtg gaatacgtcc gctgggggcc tattgggcgc ctgggcctga ttcgcaatct
      661 cccgacgctc tacgacggaa cgcatgtgaa gcatcccatg gccgagcgcc aggcggtccg
      721 gcgcgtcgaa ttcaatctgg atcgtcctgt ggacgttatg gtcgacggag aagtcctgag
      781 cctgcagtgc gaggaactcg acgtgctgcc cggagcgctc aacgtcgtgg tctgaaggcc
      841 gtgatataag aaatggacca tgtctgagga gcgaagaaaa cggcagcagg aacgttcaag
      901 cgccggccaa ggcagcgaga aaacaagaaa atatctgacc tggggtggcg ttttgattct
      961 gttcctgggt gcctttggtg ccggccgcta ttacaaaaat catcgctacg atgcgtttgc
     1021 ccgctgcctg gcgtcgaagc aggtcaagat gtacggcctc tactggtgcc cgcactgcgc
     1081 tgaccaaaag gcaatgttcg gcgcgtcgtt tcgctacgtg ccctacgtag agtgcgcgat
     1141 caaagggtcg aaggaaatgg cgcccgaatg caaaatcgcc ggggccaaac ttttccccag
     1201 ctggcagttt ggaaccaacc cgccgaaaga gggggtcttg tccctggagg aattgagtga
     1261 caagaccggg tgccgtctgc cgtgaacgac gaggggcaaa cctcattgcc cggatcatgt
     1321 ctgtcgcgct cgcgcagcat tctcttttcc gcgatcgcgg tgctgtcgct cgccggagca
     1381 atcgtgtcgg cgatctcctt gcagcggcac tacgcgaaat cagaaaccgc tttctgccag
     1441 ttcggacaaa aattcaactg cgatattgtg aaccgcagcg aatactcgag cgttgagggc
     1501 attcctgtcg ccgccatcgg tgtcgccggc tatgccgccc tgttcttgct gtccaccttc
     1561 tggagattgc gtgcggacac gccgaaccgc ttgttggccg cgtcgatcgc cgggctggcc
     1621 tttgcgctgt atctgactta tatcgaagcc tatgtgctga cgacgtggtg cattctgtgc
     1681 gtgagttcgc agcttctgat cttgctgatc agccttctcg ctgcgcttgt gaaatttcga
     1741 gacgtgaaaa cgtgaagcag cttctccgtt gtatgctgat agtgccctgt cctgatggaa
     1801 gaaatccagc aaacggaaaa tcctgcggcc ggaattgcgc ccgcgttcat tcgggccgag
     1861 tcggccaccg gagacctgcg cctgcaccca ttcaccagcc gcatcttccg caacacgcgt
     1921 ttcctcaggg tctggcttcc gccgggatac gatgattccg acaacatcac ccggcgctat
     1981 cccgtgctct atctgaatga cggccagaat cttttcgagc cgtccacctc gttcaccgga
     2041 gtcgagtggg gcgtggatga aaccgcggac cgcttgattc gcgggggcgt aattccaccg
     2101 ctgatcattg tcggtctcga caacgccgcc aaagatcgca tccgcgagta catgccgcac
     2161 cgctcgctgc accccatgat gctgcgcgtg cagggcagcc gctatcctaa cttgctgatc
     2221 aaagaagtca tgccgttcgt gttgcgcaac tatcgcgtgg ctgccggccc ggaaaacacc
     2281 ggcctcggcg gatcgtcgct cggagcactg atcgcgctct acacggcgtc ggtgcggccg
     2341 ggtctgatcg gacgcctcct gctcgaaagt ccttcgctgt gggcatcgaa ccggcagtcg
     2401 attaaagaaa gccggcacgt ccggcgttgg ccggaccgcg tctaccttgg gaccggaacg
     2461 tcggaagtcg gccggcctga gcgcgatcaa agtgtcgtgg atgacgtgcg cgagctcgcg
     2521 ggaatccttc gccgcgccgg cctcgacgac cggcgcctga agctggtcat cgaagaaggc
     2581 gctccgcacc atgaatcggc ctgggcgcgc cgttttcccg aggcgctgaa attcctcttc
     2641 gcaaacgagg ccgctgctcc gcaggtgcca aaggggtagc gtttctcagc gatcttttct
     2701 ctgcgatctc tgcgtccttt gcggttcaag ccgtttgccg gcccaggcga tcagccatgt
     2761 aactgcaagc catcatcgcc agcaccgata gctgcacgtg cgtcaatcgc gcgaacggcc
     2821 cgagaagagg cagcatgtag atcagataca gaagaattcc taaccacccg atcgctgagc
     2881 tccgcggctg gcccagcagc cagtccgcca acagcaggaa tgcaggcgcc aggatcacca
     2941 gatcgtagac cgtcaggtgc ggcgctacca gcacggtcgc cagaagcagc gacgcatatc
     3001 gcaacgccag gggcacgccc gggcgcttcc agcacgcaac cgtcatgccc agtaccgcga
     3061 tggcgctcaa aatgtacagg ctgaaagaaa gactcggcca gggcacgagc atagaccaga
     3121 atgtacgcag gcagtgcgtc tgatagggcc tgggctcgag tagtggaagt acagcccgca
     3181 cgtggagcat catgtgcagc cagcggcgca tcggttccag tccgtaataa agaactccga
     3241 tcgcaagctg cgccgcggcg gaaaggcccg caccgagcac cgttctccac gcaccggcgg
     3301 caagaagaac aacagccgcg gccagcccga gctgcggctt gaagatcagg catccaaggg
     3361 ccagaccggc caggaagcag cgcccatccc gcagttgaaa gaacatcacg gtgaagcacg
     3421 ccagcgccag ggcagaagtc tggccccagg cgatcaggtg gaagaagccg gggaacgcga
     3481 tcgcagccaa tgcggtcagg gcgccatatt cacggaggcg gggacatgcc cgccagaggc
     3541 agt
//
LOCUS       DASPUL010000461         2530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1585717, whole genome shotgun sequence.
ACCESSION   DASPUL010000461 DASPUL010000000
VERSION     DASPUL010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585717"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..1595)
                     /locus_tag="VEJ00_10990"
     CDS             complement(387..1595)
                     /locus_tag="VEJ00_10990"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXX01733.1"
                     /translation="MDTFEQIAERERKYLLHTYNRYPVVLARGKGVFLYDIEGKRYLD
                     FVAGLGVNALGHAHPRVVKAIREQAAKLVHISNLYYHEYQGPLAEKLCALSGLDRAFF
                     SNSGTEAIEGSIKLARLAGHRAGGEAKCRLVALEGSYHGRTFGALSLTGQDKHRKGFE
                     PLLEDVTFVKQNDVESLRAAVNDNTCAIVLEPIFGEGGIYECSTDFLRECRALADRHK
                     AALIFDEIQCGLGRTGTIFAFQSFGVTPDIVAIAKPIAAGLPLGAFIAKEEFATAISP
                     GQHGTTFGGGPLACRVALEFLAIVEEEKLLENVNKVGAYLQEELLGLVKKCPAAKGVR
                     GRGFIQGIQLDIPARPIVDAALAEGVLFNSTQDTVVRFLPPFLLQEKHVDKGIRVLKK
                     LLGKKQQQAA"
     gene            1760..>2530
                     /locus_tag="VEJ00_10995"
     CDS             1760..>2530
                     /locus_tag="VEJ00_10995"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXX01734.1"
                     /translation="MLTERRSCLRHEVHGPVFASFDGVTGGMILDLSEQGLAMQTTVP
                     VAADQELHLRLDLPDTDALETTGYVAWADALGRAGVRFSELPVEARQRLDQWLTRNDE
                     APSRKAPKLTLYPTFEPNRSVASRGGEKPQPQSISLEAEAGESAGQPLSNTVQYEFHS
                     LSSDLNAALRTISERARSLLRGGGAAIALIDNSSTTNKKMLCRASVGRGSPPLGTPVD
                     VNSGFSGECIRSGKAMRCDDAETDSRVSADACRRLGIRS"
BASE COUNT          478 a          819 c          730 g          503 t
ORIGIN      
        1 gcgccagacg cggcgcgaga cgggatgtcc tgaacgctgt tcatcgtaga catttctcgc
       61 ccggggacaa cttttttccc tccgcgggat tctatttaac tgaaacaacg cctctgtcgc
      121 atcactttgg ggagcttcgg ctccccgctt ttttccggtg tgtcatcctg agcggagtgg
      181 caagttcgcg aagcgaattt gccgcgcagt cgaaggagcc ctaccgcctc gaacaaactc
      241 ggagttcgca gggcattccc accccagttg cggtgagaat gccttggcgc gctcgtggcg
      301 ctcataacac ctgcggggtc cttcgactgc gtttacccac gctttgcgcg ggtgcacttc
      361 gctcaggatg acaaagtttc gagagctcac gcggcctgct gctgcttctt cccgagcagc
      421 ttcttcagca cgcgtatgcc cttgtcgacg tgcttttctt gcagcagaaa cggaggcagg
      481 aaccgcacca cggtgtcctg cgtgctgttg aagagcacgc cttcggccag cgccgcatcg
      541 acgattggtc gcgccgggat gtcgagttga attccctgaa tgaatccccg tccgcggacg
      601 cccttcgctg ccgggcattt ttttactaac cccaagagct cctcctgcag atacgctccc
      661 accttgttga cgttctccag cagtttctct tcctcaacga tcgccaggaa ctccagcgct
      721 acgcggcagg ccaaaggccc tccgccgaat gtcgtgccgt gctggccggg tgaaatcgcc
      781 gtcgcgaatt cttctttcgc aatgaaagcc cccaggggca gcccggccgc aatgggcttg
      841 gcgatggcga caatgtccgg cgtcaccccg aaactctgaa aggcaaagat cgtgcccgtg
      901 cgtcccaggc cgcactgaat ctcgtcgaaa atcaatgccg ctttgtggcg gtccgccagt
      961 gcccggcatt cgcgcaggaa atcggtcgag cactcgtaaa ttccgccttc gccaaaaatc
     1021 ggctccagca cgatggcgca ggtgttgtcg ttcaccgcgg cgcgcaggct ttccacatcg
     1081 ttctgcttca caaaggtcac gtcttccagc agaggctcga atcccttgcg atgcttgtcc
     1141 tgccccgtga gcgagagcgc gccgaacgtg cgtccgtgat acgagccttc gagcgccacc
     1201 agccggcact tggcctcgcc gcccgcgcga tgcccggcga gccgagccag cttgatcgag
     1261 ccttcgatgg cctcggtccc gctgttagaa aagaacgccc ggtcgagccc cgacagcgcg
     1321 cacaacttct ccgccagcgg cccctggtat tcgtgataat agagattcga aatgtgcacc
     1381 agcttcgctg cctgctcgcg gatcgccttg acgacgcgcg gatgcgcgtg ccccagcgcg
     1441 ttcactccca acccggcaac gaagtcgaga tagcgcttgc cttcgatgtc gtacaaaaac
     1501 acgcccttgc cgcgtgccag cacgaccgga tagcgattgt aggtatgcag cagatatttc
     1561 cgttcgcgtt ccgcaatctg ttcaaaggta tccataagaa tccgatttta ccggaaggca
     1621 ctgtggaagg cggccgggcg ttctaatcgg atgggcagga aggggatagc gatgcggcga
     1681 accccgtcat gcacttccct gagccggact ttgctccgat catcagaagt agcccggttt
     1741 ttgggatcca ggctgcacga tgctcaccga acgacgcagt tgcctgcggc atgaagtgca
     1801 cgggccggtc ttcgccagct tcgacggcgt gactggcggg atgattcttg atctcagcga
     1861 gcagggtctg gcgatgcaga ccaccgttcc ggtcgccgct gatcaggaac ttcacctgcg
     1921 cctcgatctt cccgacaccg atgcgctgga gaccaccggc tatgttgcct gggccgacgc
     1981 actcggccgg gctggagtac ggttctccga gctaccggtt gaggcgcgcc agcgcctgga
     2041 tcagtggctc actcgcaacg acgaagcgcc cagccgcaag gcaccgaagc ttactttgta
     2101 tcccacattc gagccgaacc ggagcgtcgc atccaggggt ggcgaaaagc ctcagccaca
     2161 atctatcagt ctggaagcgg aggccgggga atcggccggc cagccgcttt cgaacacagt
     2221 gcagtacgaa ttccactcac tctcctctga tctgaatgcg gcgctgcgaa ccatcagcga
     2281 gcgcgcccgc agtcttctgc gcggcggcgg cgcggctatt gccctcattg ataacagcag
     2341 cacgacgaac aagaaaatgc tgtgccgcgc cagcgttgga agaggcagcc cacccctcgg
     2401 aactccggta gatgtcaatt ccggattctc gggagagtgc atccgctccg gaaaagccat
     2461 gcgctgcgat gacgctgaaa ccgactcgcg ggtctccgcc gacgcctgcc gacggctcgg
     2521 cattcgctcc
//
LOCUS       DASPUL010000462         9277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1181788, whole genome shotgun sequence.
ACCESSION   DASPUL010000462 DASPUL010000000
VERSION     DASPUL010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9277)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9277
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181788"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VEJ00_11000"
     CDS             complement(<1..465)
                     /locus_tag="VEJ00_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXX01735.1"
                     /translation="MKKGLGFSDWASKELFRRGHLAPTFRDSDLTPFEKFPINDYDVD
                     DPGVIFENWTLTVTGMVQKPGDYKLEQIQFLPRLRQNTRHVCVEGWDVIGRFGGARLS
                     DFLAMIGADTSAKFISVACADDYYETLDMATALHPQTLLCWEMYDRPLTREHG"
     gene            925..1797
                     /locus_tag="VEJ00_11005"
     CDS             925..1797
                     /locus_tag="VEJ00_11005"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXX01736.1"
                     /translation="MYEPKIITPKSERPVLVAAPPRKRFESKQNRLMVGALALLLIAL
                     GLILYNDRDFWFPDAQDADEVLDQPVPVQQTAPAAAVPVQVPPSVKTASPAPAATPST
                     RKKQRHSKAEAKRTTTAVASNAPVLAAPPVTTSNRTVLPPLEVEVVAGDNHQTVRPGT
                     NSVRVDLHPGAPAQALSDPFAAPDTTAGVTAKASERVQLSAGTAAVVTRSVKPNYPLL
                     ARQMKVQGSVILQALIGRDGLIQDLHILSGPPILAGAAEEAVRQWHFKPHYQGSQAVE
                     TLAQITVNFTISTN"
     gene            1971..2432
                     /locus_tag="VEJ00_11010"
     CDS             1971..2432
                     /locus_tag="VEJ00_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HXX01737.1"
                     /translation="MALTISSPSFSHGADIAKKFTCDGADVSPQLSWTDPPAGTQTFA
                     LLVDDPDAPVGNWNHWTAWNVPASLRGLPEGVSKSAQLPDGSQQGLNDFRKPGYNGPC
                     PPPGKPHRYYFKIFALDAKLDLKPSASKRELEAAMKGHVLAQGEWMGRYGR"
     gene            complement(2504..4024)
                     /locus_tag="VEJ00_11015"
     CDS             complement(2504..4024)
                     /locus_tag="VEJ00_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXX01738.1"
                     /translation="MKQTGIVILAVLFSAAAVSGQQAASDGSSADIVYAQPGQLVDAG
                     GFRLNLYCTGSGSPAVVFDSGWGDWAPAWSKVQPEIAKWTRACSYDRAGVGFSDPGPM
                     PRTSVRIAGELRTALHNAGIGGPYILVGSAFGGYDIRTFADLYMDEVAGMVMDDADPV
                     DLEAKAMQEEDQRGLARIPKDMRECRDVIAEHKALPATCAQRFFRGLPESAWSQELNA
                     KVLEIAQTKAAMYDSYASEMEQTPADQTYLQQHRRSLGSRPVRVLTSGNHGVGHLESK
                     PPDTPKHLEYERETALAQSRWLELSSDAKQIFAHHSSEYIQFDEPETVIDAVREVYDQ
                     SGGSAQPQQVPTLASTMDRQLSRVEDLIVGVAEAMPEDKFNFTPENLKIPESDYKGVF
                     TFAGQIKHVAAGNYFIWSTVAGEKLDEKLFNDGRGPDNLKTKAEVIQFLKDSFALGHR
                     AAATLTPENMLQSDGKSTRISMATFVIAEAYNHYGQMVEYLRMNGIVPPKSRGKSD"
     gene            complement(4067..5332)
                     /locus_tag="VEJ00_11020"
     CDS             complement(4067..5332)
                     /locus_tag="VEJ00_11020"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXX01739.1"
                     /translation="MKASRLLDPRTGNVISPAAVLIEDGRIKAVGPPAHVQAPAGAKT
                     IDLGSATLLPGLIDSHTHLLLDVIVPPEEEAKRHLNGDFAPGLLLTIVESPIKRALMG
                     AQLAREDLESGFTTVRNLGHSGIDGDTSLRDAINAGRLPGPRILAAGRKMIGQGDYLQ
                     DLNPALADAILQQEFILITSPDQARHAVLQNVFYNVDVIKVSLGDDTTTEELKAVVDE
                     AHRQQLKVAVHAFESSNIQVAIDAGADSIEHGNQVTDDQLKQMREKGTFLDLTPTFYG
                     GFFTKITEPSIVISPAGRASNASADARRDKRYQEFVQRVLKSGVKFAAGSDMCWFYPG
                     KTRGQASTAALVNLRDAGMPSLDVLRAITTNAAEMLGWQDRIGAVEPGKFADLVAVSG
                     DPIADITELERVRFVMKDGQVVRNDLASH"
     gene            complement(5444..7243)
                     /locus_tag="VEJ00_11025"
     CDS             complement(5444..7243)
                     /locus_tag="VEJ00_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX01740.1"
                     /translation="MQSTMQWCSKQFRNGILAITLLIAFGLSLRSQVQDQAPGPARSR
                     TDETLALWNAIGNKLVAMAEDFPEDKYDFKVQKDERTFALNLLHAAALDFVLIRRISG
                     SNLGPDFGEGDNPTRDQFKTKSDIVKFLKEAVADGAKVIQDQGDAGLDKTTKFFGNRL
                     AHNSYIWAFVIEHSAEHYGQLVVYYRANNLIPPDSRRQAQTAQPASAAQAVDLKGPDG
                     TILKASYFAAAKPGPGVLLFHQSNRTRQSWDDLARQLAAAGINTLTIDTRGYGESGGK
                     KQDREQYEDSDLDVAFDYLVSQPGVTRESIGVGGAGWLGVDSSVETARRHIAAVKSMV
                     LISGETLLPQLRFMRQASQLPGLFVVSDDDEYPPTVEVMEWLYDCSSSPQKQFIHYIG
                     PKAPWLWYETSDPSKVPATGSHGTDLFQRHPELPAAIVDWFVTTLIRTPGRAPADAMA
                     SAAILDEIETAGGVAEATRKLLEARKRDPKAQLWPEVNVDIIGEDHTRLAEVEKKAGQ
                     LHEAEIERKAGIEIFKLNLLAYPDSADAHSNLADAYLANGQKDLAREYAEKALAMIDS
                     HKAPLSSWSDTGPRRAEVRNDVEDVLKNVNAVH"
     gene            complement(7261..7878)
                     /locus_tag="VEJ00_11030"
     CDS             complement(7261..7878)
                     /locus_tag="VEJ00_11030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX01741.1"
                     /translation="MALLDPAAVSAVAALEDDPFYRSICAGFAGDQTRRRHALAQYFD
                     YSIQEGTAFGRSLHLPKPAQGVAVWRLPQPAEVQAHAAHNKRLFLQTTLDAEGCANYH
                     RIVDFMSGKAAGLMDRKAWYLSIVAVDPMAQRQGLGRRLLEPTIAEADSVSATCYLET
                     FSVRNLPFYERLGFAAEARFTEPTTGADYVVMVRHPGTAKARGTP"
     gene            complement(7883..8854)
                     /locus_tag="VEJ00_11035"
     CDS             complement(7883..8854)
                     /locus_tag="VEJ00_11035"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HXX01742.1"
                     /translation="MHEHLARALYYLGVHLLYASMVWAAAWVLTSWVRASATTKYWIW
                     LATSLNFMLPLGAVLDRIWATHLSWARPLGAIGDVGARLSESTTLVALLGGSWLVGAT
                     LMFIRLCLRVHAERLEARIGASESALELNPGFLARGVPVKFAGMRQAPAVGGLVNPHI
                     SLPRGIDRLLTKPELNAVLLHELTHARRHDNLARLIHEAALCLLWFHPLVWITGSRLS
                     LYRELSCDESVIRKARGRDLVSALAKLASSEEPLLLQASASSFLTHRLERLTASPAQR
                     SGFAANTAMIAVFCAALLAGVFGTIAHTACCFVPHGQSHIAHACPRT"
     gene            complement(8847..>9277)
                     /locus_tag="VEJ00_11040"
     CDS             complement(8847..>9277)
                     /locus_tag="VEJ00_11040"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HXX01743.1"
                     /translation="VVEFPYCVVDMKKKHLSQAETRILEQYWKLGTCSVREILDSLPE
                     DERVAYTTVQTLVYRLEQKGALRKVKKIGNAQLFEPVIDQHEFRSGLVRDLLDLFGGS
                     PRLLVSNLLETGTISLKDLKALQNAAGAKSNRGRGGKAHA"
BASE COUNT         1818 a         2853 c         2745 g         1861 t
ORIGIN      
        1 accgtgctcg cgcgtgagcg gacggtcgta catttcccag cacagcaacg tctgcggatg
       61 cagcgccgtc gccatgtcca gtgtctcgta gtagtcgtct gcgcatgcga ccgagatgaa
      121 cttcgccgaa gtgtcggctc cgatcatggc aagaaaatcc gaaagccgtg ctccgccgaa
      181 acgtccaatt acgtcccagc cctcgacgca gacgtgcctt gtgttctgcc ggagccgggg
      241 gaggaactga atctgctcca atttgtagtc accgggcttc tgcaccattc cggtcacggt
      301 caaggtccag ttctcgaaga tcacgccggg atcgtcaacg tcgtagtcgt tgatgggaaa
      361 cttctcgaac ggcgttagat ccgaatcgcg gaaggtcgga gccaggtgac ccctccggaa
      421 cagctctttc gaagcccagt cgctgaagcc gaggcccttc ttcaagagta aaggctggaa
      481 cgcagggatg gcgaaagccc ctaaggcaat gacaggtacg actttaagca actcccggcg
      541 ggatacccgc gcaaactgac gctcgctcat gccaggacgc gcggctctgg tctctgagaa
      601 acttgcctcc tccgcttccg ccctttcggc gcctaccgcg tcgaccgagg aacacgagga
      661 ttgcttcccg gcgtcattca ggtcggctgg tttttccgcg ttattcggcc ctaagttctc
      721 tgactccata gtcctccgcc accgggtccc agaatgggat tcgagagaac cccacaagtt
      781 acgcacaaaa tagcacgggg cggctcaaaa gaccccgatg agactctcgg gacggctccg
      841 gatgcgcggt tttgctggca tttcgagggt tttcgaggca tactattcat cggtgttctc
      901 ccccaggaac ggcccaggtc ctgcatgtac gaacccaaga taatcacacc gaagtccgag
      961 cgtccggtcc tggtggcggc gccgccgcgg aagcgtttcg aaagcaagca gaaccgcctg
     1021 atggtgggag cgttggccct gctgctgatc gccctggggt tgattctcta caacgatcgc
     1081 gacttctggt tccctgacgc acaggacgcg gatgaggtgc tggatcagcc ggtgccggtg
     1141 cagcaaactg ctccagcagc agccgttccc gttcaggtac cgccgagtgt aaagacggcc
     1201 agccccgcac cggcagctac gccatccacc agaaagaagc agcgtcactc caaagcggag
     1261 gcaaagcgca ccacaacggc agttgcgtcc aacgcgccgg ttttggctgc cccgccagtc
     1321 accacgagca accgcaccgt gttgccgccc cttgaagtgg aagtcgtagc tggcgacaat
     1381 catcaaactg tccgccccgg taccaactcc gtgcgggtgg acttgcatcc cggcgcaccc
     1441 gcccaggcgc tttcggatcc gtttgcagcg cccgacacga cggcaggtgt aactgccaaa
     1501 gcctccgagc gtgtgcaatt gtccgcagga acggctgctg tggtcacgcg ttcggttaag
     1561 ccgaactatc ctcttctggc gcgccaaatg aaagtgcagg gttcggtgat cctgcaggca
     1621 ctgattggcc gcgacggact gatccaggat ttgcacattc tcagcgggcc tccgattctg
     1681 gctggcgccg ccgaggaagc ggtacggcag tggcacttca agccccacta ccagggcagc
     1741 caggccgtag agactctggc ccagatcacc gtcaacttca ccatttccac caactgaact
     1801 gcaggccgaa acgcgtggac actcccccgc aagccgcgat ggccaaacac cggttccagc
     1861 aaatccgcac aggactgctt gccggcgctg tagcggttgg gctgctcacg ctggcgttcc
     1921 ggcccaggtc agcggcaaaa ccgtacttgt ctccgaaagg agaaccggct atggctctga
     1981 caatttcgag tccgagtttt tcccacggcg cagacatcgc gaagaagttc acatgcgatg
     2041 gagccgacgt atccccccag ctctcatgga cggacccacc ggcgggaacc cagaccttcg
     2101 cgcttctggt ggatgacccc gacgcacctg tcggaaactg gaatcactgg accgcgtgga
     2161 atgtgccggc aagcttgcgc ggcctgcccg aaggcgttag caaatccgcg caactcccag
     2221 acggatcgca acagggcctt aacgatttcc gtaagccggg atacaacggc ccttgccctc
     2281 cgccaggcaa gccgcatcgc tactatttca agatctttgc cttggatgca aagctcgatc
     2341 tcaagcccag tgccagcaag cgcgaactcg aagcagcgat gaaggggcac gtcctggccc
     2401 aaggcgagtg gatggggcgc tacggacgct aagaccggcc gacgctcgac caaccgggtc
     2461 gcgaacgccg aggctaacac cccgaaggtg ggcgccgatc cctttagtcc gacttccctc
     2521 tgctcttcgg cggcacgatc ccattcattc tcagatactc caccatctgt ccgtagtggt
     2581 tgtaggcctc cgctatgacg aacgtcgcca tggagatgcg cgtcgatttc ccgtcgctct
     2641 gcagcatgtt ttccggcgtc aatgttgccg ctgccctgtg acccagagcg aacgagtctt
     2701 tcaggaactg aatgacctcc gctttcgttt tcagattgtc ggggccgcgg ccatcgttga
     2761 acaatttctc gtccagtttt tcgcctgcga ccgtcgacca tatgaaatag ttccccgctg
     2821 cgacgtgctt gatctgccct gcaaacgtaa acacaccttt gtaatcgctc tccggaatct
     2881 tcaaattctc gggagtgaaa ttgaacttgt cctccggcat ggcttcggcg acaccgacaa
     2941 tcagatcttc gactcggctg agctggcgat ccatcgtcga agctaaggtt ggcacctgct
     3001 gtggttgggc tgatcctccc gattggtcat acacttcacg gacggcgtcg atcacggtct
     3061 ccggttcgtc aaactggatg tattccgagc tgtggtgggc gaagatttgt ttcgcatctg
     3121 aagatagttc gagccaccgt gactgggcca gcgcggtttc gcgctcatat tcgaggtgct
     3181 tcggcgtgtc cggaggcttg ctctccaggt gcccgacgcc gtggttcccg gacgtcagca
     3241 cgcggacggg ccgggagccg agtgagcggc ggtgctgctg cagataggtt tggtccgcag
     3301 gtgtttgttc catctctgag gcataggagt catacatggc agctttggtt tgcgcaatct
     3361 ccagcacttt ggcgttcaac tcctgagacc acgccgactc cggcaaaccc cggaaaaaac
     3421 gctgagcgca ggtcgcaggc aaggccttgt gctctgcaat cacgtctcgg cactcgcgca
     3481 tatccttggg aatcctggcg agtccccgtt gatcctcttc ctgcatcgct ttcgcttcca
     3541 ggtccacggg atcggcatca tccatgacca tgcccgcaac ctcatccatg tacaggtctg
     3601 cgaacgtgcg tatgtcgtag cccccgaacg cggatcctac caggatgtag ggcccgccga
     3661 tgccagcatt gtgtagcgcc gtgcgcagct ccccggcgat gcgcacgctg gtgcgcggca
     3721 tggggccggg atcgctgaat cctacgccag cccggtcata gctgcaggcc cgcgtccatt
     3781 tggcgatctc cggctgcacc ttcgaccacg ccggcgccca gtctccccat ccggaatcga
     3841 agacgaccgc gggtgagccg ctccccgtgc aatagagatt cagccgaaat ccacccgcgt
     3901 caaccaactg gccgggctgt gcgtaaacga tgtcggcgga cgaaccgtcg ctcgctgctt
     3961 gctggccgga gacggcggcc gcggagaaaa gcactgccag gatcacgatt cctgtttgtt
     4021 tcatctcacc acctcgatgt gttgaaatca cagattcatc cgcgtctcaa tgcgacgcta
     4081 agtcgtttct cacgacctgt ccatccttca tcacgaatcg aacgcgttcc agttccgtga
     4141 tgtcggcaat tggatctcca gagacagcga ccaagtcggc gaatttgccg ggctcgacgg
     4201 caccaatacg atcctgccag cccagcattt ctgcggcgtt ggtggtaatc gcacgaagaa
     4261 cgtcgagcga tggcatcccg gcatcgcgca aattcacaag ggccgccgtg ctcgcctggc
     4321 cgcgcgtctt tcccggatag aaccagcaca tatcggatcc ggcggcaaac ttcacgccgg
     4381 atttcagcac gcgctgaacg aactcctggt agcgcttgtc gcgccttgca tcggcgctgg
     4441 cgttagaagc gcgcccggcg ggtgagatca caatgcttgg ctcggtgatc tttgtgaaga
     4501 aaccgccata aaacgtcggc gtcaggtcga gaaacgtgcc tttctcgcgc atctgcttga
     4561 gttggtcgtc ggtgacctga ttgccgtgct cgatggagtc cgcgcccgca tcgatcgcga
     4621 cctggatgtt ggatgactcg aaggcgtgca ccgcgacttt cagctgctga cggtgcgctt
     4681 cgtccaccac ggccttcagt tcctcggttg tggtgtcatc acccagcgac actttgatca
     4741 catcgacgtt gtagaagaca ttctgcagca ccgcgtgcct tgcctggtcc gggctggtga
     4801 tcaagatgaa ttcctgctgg aggatggcgt cggcgagagc aggattgagg tcttgaaggt
     4861 agtcgccctg gccgatcatt ttccgtccgg cagccagaat tctcggaccg ggaaggcgtc
     4921 cggcgttgat cgcgtcgcgt agggatgtgt cgccgtcgat gcccgagtga cccaagttgc
     4981 ggaccgttgt gaagccgctc tccagatcct cgcgcgccag ctgcgctccc atcagcgcac
     5041 gtttgatggg tgactctacg atggtcagca acaggcccgg agcaaaatct ccgttgaggt
     5101 ggcgcttcgc ctcctcttcc ggcggcacaa tcacatcgag cagcaaatgc gtatgactgt
     5161 cgatcaggcc gggcagcaag gttgcgctgc cgagatcaat ggtcttggcg ccggctggcg
     5221 cctgtacgtg cgcgggagga ccaacggcct tgatcctgcc gtcttcaatc aacacggctg
     5281 cgggagaaat cacattgccg gttcgcggat cgagcagccg ggaagccttc accagagttg
     5341 ggctagccgg tggtgattga gccgtgagtc ggaggaatgc aagaggaaca accagtatgc
     5401 aagctacgac agcgaaccgg tttcgcataa ctcgcgcctc cagctagtgg acagcattca
     5461 cgttcttcag cacatcctcg acgtcattgc gaacttcggc gcgtcgcggc ccggtgtcgg
     5521 accaggaaga taaaggcgcc ttatgggaat cgatcatcgc caaggctttc tcggcatact
     5581 cgcgggccag gtctttctgt ccatttgcca gataagcatc agcgaggtta gagtgcgcat
     5641 cggccgagtc gggatacgcg aggaggttga gtttgaagat ctcaatgccg gctttcctct
     5701 cgatctcggc ctcgtgcagc tggccggctt tcttctcgac ctcggccaag cgcgtgtggt
     5761 cctcgccgat gatgtccacg ttgacttccg gccacaactg cgctttggga tctcgcttcc
     5821 gggcttcgag cagtttgcgc gtggcctcgg ccacgccgcc ggcggtctcg atttcgtcca
     5881 ggattgcagc cgacgccatg gcatcggctg gggcacggcc gggcgttctg atcagcgtag
     5941 tgacgaacca atcaacgatg gccgcgggca gttccggatg gcgttggaac aaatccgttc
     6001 catgacttcc ggtcgctggg actttgctcg ggtcagaggt ctcgtaccac agccacggcg
     6061 ccttgggccc gatgtaatgg atgaactgtt tttgggggct agaggaacag tcatagagcc
     6121 attccatcac ctcgacggta ggaggatact cgtcatcatc ggacaccacg aacagcccag
     6181 gcagctgcga agcttgccgc ataaaccgca attgcggcag caaggtttcc ccggagatca
     6241 gcaccatcga tttcacggca gcgatatgac ggcgtgccgt ttcaacagaa ctgtccacgc
     6301 ccagccatcc ggcgcctccc acgccaatcg actcgcgggt cacgccgggc tgcgaaacca
     6361 gataatcgaa ggccacgtcc agatcgctgt cctcgtactg ttctctgtcc tgcttttttc
     6421 cgccgctctc gccatatccg cgggtatcga tggtgagcgt gttgatgccc gccgctgcca
     6481 gttgtcgagc cagatcgtcc caggactgac gcgtacggtt actctgatga aacagcagca
     6541 cgccggggcc tggcttggcg gcagcgaagt agctcgcttt gagaatggtt ccgtcgggtc
     6601 ccttcaggtc caccgcttgg gcggccgacg caggctgtgc ggtttgagcc tggcgccgcg
     6661 aatctggcgg gatcagattg ttcgcgcggt agtagacgac cagttgcccg taatgttccg
     6721 cgctgtgctc gatgacgaac gcccagatgt aggagttgtg ggccagccgg ttgccgaaga
     6781 atttcgttgt cttgtccagc cctgcgtcgc cctgatcttg aatcactttg gctccgtcgg
     6841 caacggcctc cttcaggaac ttgacgatgt cggatttggt cttgaattga tcacgcgtag
     6901 gattgtcgcc ctcgccgaag tcaggaccga ggtttgatcc ggaaatcctc cgtattagca
     6961 cgaaatccaa tgctgcagcg tggaggagat tcagggcaaa ggtgcgctcg tccttctgca
     7021 ccttaaaatc gtacttgtct tctggaaaat cctcggccat ggcgacgagt ttgttgccaa
     7081 tcgcattcca cagtgccagg gtctcatcgg ttcgggagcg cgccgggccg ggcgcctgat
     7141 cctgaacctg actgcgcaga ctgagtccga acgcgatcag tagagtgata gcgagaatcc
     7201 cgttccggaa ctgcttcgaa caccattgca tcgtggattg cattgcaggc tcctccctgg
     7261 ctaaggtgtg ccgcgggctt tggccgtgcc tggatgccgg accatcacca cgtagtcggc
     7321 gccagtggtc ggctctgtga atcgcgcctc ggcggcaaac ccgaggcgct cgtaaaacgg
     7381 aagattgcga acgctgaacg tctccaggta acaggtcgcc gagacgctgt cggcttcggc
     7441 aatcgtcggc tccagaagcc tccggccgag cccctgtcgc tgtgccatcg gatcgacggc
     7501 gacgatcgag agataccagg ccttgcggtc catcaagccg gcggctttgc cgctcatgaa
     7561 atcgacaatg cggtggtagt tggcgcagcc ttcggcgtcg agcgtcgtct gcaggaacag
     7621 acgtttatta tgggcggcgt gcgcctggac ctccgcaggt tggggcaggc gccacacggc
     7681 gacgccctgc gccggcttgg gcaaatgaag cgagcggccg aaagccgtgc cttcctgaat
     7741 cgaatagtcg aagtactgcg ccagcgcatg cctgcgacgg gtctgatccc ccgcaaagcc
     7801 ggcacagatt gagcgataaa acgggtcatc ctccagcgct gcgactgcac tcaccgcagc
     7861 ggggtcgagt aatgccacat gcttatgttc gcgggcaggc atgtgcgatg tgggactggc
     7921 cgtgagggac gaaacagcaa gcggtgtgcg ctatcgtccc gaaaactccc gcgagcagag
     7981 cggcacagaa cacagcgatc atcgcggtgt ttgcggcaaa gcccgagcgt tgcgccggag
     8041 aagcggtcag ccgctcaagc ctatgggtca agaacgacga cgcggacgcc tgcagtagca
     8101 aaggctcttc cgagctggca agcttggcca acgcagagac caggtctcga ccgcgagcct
     8161 tccgaatgac cgattcgtcg caggaaagct cccggtacag agaaaggcgg gagccggtaa
     8221 tccacacgag cggatgaaac cacaacaggc aaagagccgc ttcatggatc aggcgagcca
     8281 gattgtcgtg cctcctggca tgggtcaact cgtggagaag gacggcgttg agttccggct
     8341 tggtcagcaa tcggtcaatt ccgcggggaa gtgaaatgtg aggattcacc agaccgccga
     8401 ctgccggagc ctgccgcatc ccggcaaact tcacggggac accccgagcc agaaagccgg
     8461 gattcagctc cagagcgctt tcgctcgcgc caatccgagc ttcgagtctc tcggcgtgta
     8521 cccgcaggca aaggcgtatg aacatcaggg tcgcgcccac cagccaagac ccacccagca
     8581 gtgcgaccag cgtggtgctt tccgagagac gcgcccctac gtcgccgatg gcgcccaggg
     8641 gcctggccca cgagagatgc gtcgcccaga ttctgtcgag gaccgcaccc agcggcaaca
     8701 tgaaattgag cgatgtcgct agccagatcc agtatttcgt cgtcgcgctg gcgcgtaccc
     8761 aggacgtcag aacccacgct gcggcccaga ccatcgaggc atacagaaga tgcactccca
     8821 ggtagtacag cgcccgtgca aggtgctcat gcatgcgctt tgcctccacg gccgcggttg
     8881 ctcttagcgc ctgcggcatt ttgcagggcc ttcaggtcct tcagcgaaat tgtgccggtt
     8941 tcgagcagat tggaaaccaa cagacgtggc gagccgccga acagatccag aaggtcccgc
     9001 acgaggccgc tgcggaattc gtgctgatcg atgacgggct cgaataactg ggcgttgccg
     9061 attttcttta ccttgcgcag cgcgcccttc tgctccagcc ggtagaccag cgtctgcacg
     9121 gtcgtgtaag ccacgcgctc atcttcgggc aggctgtcga gaatctcgcg gacggagcag
     9181 gttcccagct tccagtactg ctccaggatt cgcgtctcgg cttgggagag atgcttcttt
     9241 ttcatatcta ctacgcagta cggaaattct actacac
//
LOCUS       DASPUL010000463         3331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1585876, whole genome shotgun sequence.
ACCESSION   DASPUL010000463 DASPUL010000000
VERSION     DASPUL010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585876"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VEJ00_11045"
     CDS             <1..868
                     /locus_tag="VEJ00_11045"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXX01744.1"
                     /translation="AQVIRFRDGECYVQIQENVRGADVFVMQPTCRPVDEHLMELLLM
                     IDALKRASARRITAVIPYFGYARQDRKDKPRSPISSKLVADLLTTAGAHRALIVDLHT
                     PQLQGFFNIPVDHLFASPVLVDHFRKLALPNLTVVSPDAGGVERARFFAKKVDAALAI
                     VDKRRVEMNVAEIMHVIGDVRGRTCLVIDDLIDTAGTLVKTAMALMDNGAISVYACAS
                     HAVLSGPAVENIKNSVIKEVVVTNTIPLDATADAARTEKGGKITVLSIAGLIGRAIQA
                     NHEETSVSKLFM"
     gene            970..1695
                     /locus_tag="VEJ00_11050"
     CDS             970..1695
                     /locus_tag="VEJ00_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HXX01745.1"
                     /translation="MPTALEQNTLEAQSREAGTKNEARRVRREGKIPGVVYGAGKDSF
                     AVSVDPRHVLRILHSESGHNTIFDLTLNGERTKAMIVDWQYEPIRGTLLHIDLKRIAM
                     DKVLRVSVPINLTGEAAGVKTEGGILEHILREVEIECLPSDIPSHIDVDVAHLTFGKV
                     LRVSDLPHSEKLKFLTDANQPVAHVTAVKEEVVATPEAAAAEAAAAPAEPEVIKKGKQ
                     ETEEEGAEAPAEKPEKAEKKEKK"
     gene            1809..2411
                     /gene="pth"
                     /locus_tag="VEJ00_11055"
     CDS             1809..2411
                     /gene="pth"
                     /locus_tag="VEJ00_11055"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXX01746.1"
                     /translation="MKLIVGLGNPGIEYQFTPHNLGFLAIDRIAGSLGVEVRNRQCRA
                     LTARAVIADQPVLLAKPETFMNLSGVSVRELVRQYEAKPESDLIVIQDELDFPLGTLR
                     IHTRRSSAGHNGIESIIGALDTQDFLRIRIGVAPERKVSDGPSYLLAPMKKKDLQVVD
                     EMLDTAEEAVKMILKDGPAAAMNRFNRKEDKDQGPGARDQ"
     gene            2473..2883
                     /gene="rpsF"
                     /locus_tag="VEJ00_11060"
     CDS             2473..2883
                     /gene="rpsF"
                     /locus_tag="VEJ00_11060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXX01747.1"
                     /translation="MNRTYELMFIVRPDMVDEDLDKLISTLQTAVPASGGTVQKVEKM
                     GKRRLAYTVRRFNDGIYVLMIVEGGGAVIHELERRLRVSEPVIKFLTVRVDEEQKRLA
                     KIKALRDARKKVSAQPAAAAEGASAPAAEAPATA"
     gene            3049..>3331
                     /gene="rpsR"
                     /locus_tag="VEJ00_11065"
     CDS             3049..>3331
                     /gene="rpsR"
                     /locus_tag="VEJ00_11065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXX01748.1"
                     /translation="MAEETKAPEQAASSERPRSEGRPEGRPGGDRPGGDRPRFGGGGR
                     GPGGPPREGGRKFFRRKKVCKFCVEKIEDINYKDYRLLGQFVAESGKIVP"
BASE COUNT          726 a          957 c         1077 g          571 t
ORIGIN      
        1 ggcgcaggtc attcgcttcc gcgacggcga gtgttatgtc cagattcagg agaacgtgcg
       61 cggcgccgat gtgttcgtca tgcagcccac gtgtcgcccg gtggatgagc atctgatgga
      121 gttgctgctc atgatcgacg cactcaagcg ggcgtcggca cggcgcatca cggcagtgat
      181 tccgtacttc ggctatgccc ggcaggatcg caaggacaag ccacgcagcc cgatctcatc
      241 gaagctggtg gctgatctgt tgaccaccgc gggcgcgcac cgggcgctga ttgtggattt
      301 gcatacgccg cagttgcagg gctttttcaa tattccggtc gaccacctgt tcgcctcgcc
      361 ggtgctggtg gatcacttcc ggaagctcgc tttgcccaac ctgacggtgg tctcgcccga
      421 cgccggtggc gtggagcggg cccggttctt tgccaagaag gtcgacgcgg ccctggccat
      481 cgtcgacaag cgccgcgtcg agatgaacgt ggccgaaatc atgcacgtga tcggcgacgt
      541 ccgcggtcgg acctgcctgg tcatcgatga cctgatcgac acggcgggaa cgttagtaaa
      601 gaccgccatg gcgctgatgg acaacggcgc gatttccgtc tacgcgtgcg cctcgcacgc
      661 cgtgctctcg ggcccggcgg tggagaacat caagaattcg gtgatcaagg aagtcgtggt
      721 caccaatacg atcccgctcg acgccaccgc cgatgcggcg agaacggaaa aaggcggaaa
      781 gattaccgtg ctctcgattg cagggctgat tggccgcgcg attcaggcca accacgagga
      841 gacgtcggtc agcaagctgt tcatgtaggc acgagctatg tagggacggg cgccctcgcc
      901 cgtccgggcc gagcgtagct cggctggctc tgaggatcac aattggactt agggtaggaa
      961 ttgaatctta tgccaactgc attggaacaa aacacactgg aagcgcaatc ccgcgaggcg
     1021 ggaacgaaaa acgaggcccg gcgcgtccgc cgcgagggga agatccctgg cgtcgtctat
     1081 ggagccggca aggactcgtt cgcggtcagc gttgacccgc ggcacgtgtt acgcattctc
     1141 cactccgaga gcggacacaa tacgatcttc gatctcacgc tcaacggcga gcgcaccaag
     1201 gcgatgattg tcgactggca gtatgagccc atcaggggca cgctcctgca catcgacctg
     1261 aagcgcatcg ccatggataa ggtgctgcgc gtaagcgtac ccatcaacct gaccggagaa
     1321 gccgccggcg tcaagaccga gggcggcatt ctggagcaca ttctccgcga agtcgagatc
     1381 gagtgtttgc cgagtgacat cccgagccat attgatgtcg acgtcgctca cctcacgttc
     1441 ggcaaggtgc tgcgcgtttc tgacttgccg cattcggaaa agctgaagtt tctaaccgat
     1501 gcgaatcagc cggtcgcgca cgttacggcg gtgaaggaag aagttgtcgc cacgcctgaa
     1561 gctgcggcag cggaagcggc agccgctcct gccgagcccg aggtcatcaa gaagggcaag
     1621 caggagaccg aggaagaagg cgccgaagct ccggccgaga agccggagaa ggccgagaag
     1681 aaggaaaaaa agtaagactg gccacagatt gtacggattc ccacggagag aacccggttt
     1741 cgctgtatcc gtgatgatcc gcgaaaatcc gtggcgaatg gttgggcttt ttgcggggtt
     1801 cgtgaggcgt gaaactgatc gtcggtcttg gcaatcccgg catcgagtac cagttcacgc
     1861 cccataacct gggatttctg gcgatcgatc gcatcgccgg cagtctggga gtggaagtga
     1921 gaaaccggca atgccgggcg ctgaccgcgc gagcagtaat tgccgatcag ccggtgctgc
     1981 tggccaagcc cgagacgttc atgaacttga gcggcgtttc agtgcgagag ctggtgaggc
     2041 agtacgaagc gaagccggaa tcggacctga tcgtgattca ggacgagctc gattttccgc
     2101 tgggcacgtt gcgcattcat acgcggcgca gttcggcggg gcacaacggc atcgagtcga
     2161 tcatcggcgc cttggacacg caggatttcc tgcgcatccg gatcggcgtg gcgccggagc
     2221 gaaaggtgtc agacggtccg agctacctgc tggcgccgat gaagaagaaa gacttgcaag
     2281 tcgttgacga aatgctcgac accgcggagg aagcggtgaa gatgatattg aaagacggtc
     2341 cggcagcggc catgaaccgc ttcaaccgga aggaagacaa agatcaggga ccaggggcca
     2401 gggatcagta aagtccgttt tactggcccc tgacctctgg cccctgatcc ctgaaaggtt
     2461 ttggagactc gcatgaatcg tacttatgag ctgatgttca tcgtccggcc cgacatggtg
     2521 gacgaagatc tggacaaact gatttccacg ctgcaaacgg cggtgcccgc ctccggcggc
     2581 accgtgcaaa aggtcgagaa gatgggcaag cgccgtctcg cctacaccgt gcgccgcttc
     2641 aatgacggca tctacgttct gatgatcgtc gagggcggcg gcgcggtgat ccacgaactg
     2701 gagcgccgcc tgcgcgtgag cgagccggtg attaagttcc tcaccgtccg cgtcgacgaa
     2761 gaacagaagc gcttggccaa gatcaaggcc ctccgcgacg cccgcaagaa agtaagcgcg
     2821 cagcccgcgg cagcggcgga aggcgcgtcg gcccccgcgg cggaagcccc ggcaacggcg
     2881 taagaaccga aagcaggttt tcgcagcgca ctttgcggct gttcctccgc ggccttcgcg
     2941 gtaaaaagtt tttgagcgca gagatcgcaa acgaaatgcc gcgaagagcg cgcagaagac
     3001 ctgagcaaaa cccggggcag aacagccggt aaaagattcg aggagaatat ggcagaagaa
     3061 accaaagcac cagagcaagc ggcatccagc gagcggcccc ggtctgaagg gcgtccagaa
     3121 ggccgtcctg gcggcgatcg tccaggcggt gaccgcccgc gtttcggcgg cggcggacgc
     3181 ggacccggcg gccctccccg cgaaggcggg cgcaaattct tccggcgcaa gaaagtttgc
     3241 aagttctgcg tcgaaaagat cgaagacatc aactacaagg attaccgcct gctcggccag
     3301 ttcgtcgccg aaagcggcaa gatcgtgccg c
//
LOCUS       DASPUL010000464         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1039779, whole genome shotgun sequence.
ACCESSION   DASPUL010000464 DASPUL010000000
VERSION     DASPUL010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039779"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1780
                     /locus_tag="VEJ00_11070"
     CDS             <1..1780
                     /locus_tag="VEJ00_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HXX01749.1"
                     /translation="LQRELDRRRAARPLVPSQVVPARSTATDVERRPDSPGDVGGIPS
                     AVAQTGIERVLSPAHPESVPERVHRDSPVPSPAEAEPEVNQRGDSARKPRTTLSRIAG
                     SFKLPPSSLLHRPDEQQAVDAGELKLLAQVLTEKYAEFDVHGQVTQINPGPVVTTFEF
                     KPEAGIKYSRITNLTEDLCLALKAESILVERMAGKSTVGIQVPNREREIIWLRENLES
                     AEFIGSKSRLTLSLGKDINGRIAVADLAGMPHLLIAGSTGTGKSVAINAMIMSILYKA
                     TPHQVKLILVDPKRLELGNYEGVPHLYTPIITEPKLAANALRNAVREMERRLKLLAEK
                     GVRNIDQYNRLFEDEETLSLFGDDPEERPIPYIVIIIDELADLMMLDSGNVEESVTRL
                     AQMARAVGIHLVLATQRPSVDVITGLIKANFPARISFRVATKVDSRTILDANGAEALL
                     GRGDMLYLPAGSARVHRLHAPLVTEKEIAAVVEFWRSQGDADYQEAFLEAPPEEREAG
                     ERAAGELGEDENDPLFADAVRLVVEFGKASTSLLQRRLRIGYGRAAHLIDLMERDGIV
                     GAADGPKPREVLKRPDWISEVEESMR"
     gene            1933..>3465
                     /locus_tag="VEJ00_11075"
     CDS             1933..>3465
                     /locus_tag="VEJ00_11075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HXX01750.1"
                     /translation="MSRSTDPLLALRRYWGYDSFRPLQQRIIQSLLSGRDACVVMPTG
                     GGKSLCYQLPAVVLEGTVVVISPLIALMQDQVAQLRQMGIPAAGLNSMLAGNQQFEVM
                     QAAREGAYRLLYLSPERLARPDTTEWLRQVPVSFFAIDEAHCISEWGHEFRPEYRQLS
                     RLRTEFPERPIAAFTASATRRVRHDILEQLQLRDPHKYIASFHRRNLRYVARQSDAVS
                     QPQLLVRALRQLGDGNAIVYSPTIARVERTVAMLEEEGIPSVAYHGRMETEERRRNQE
                     RWMSDEVRVLVGTIAFGLGINKAGVRAVIHLALPKSLEQYYQEAGRAGRDGRPADCIL
                     LWQKRDTALLAHFIGQMEDPAEKERAWQRYRDIRDFAESHSCRHRQVCRHFGETPKWA
                     SCGACDACGSEPEWLKRPVTEELPRRTRWKPPSPPAAPGTTGAPGPRAAEIDQALWDY
                     LREWRRSTARQQRIPAYIVLHDRSLEELCRVRPRTLRDLTGVPGFGKRKVELYGRQIL
                     DAL"
BASE COUNT          642 a         1076 c         1171 g          576 t
ORIGIN      
        1 gttgcagaga gaactggacc gccggcgtgc tgcccgaccc ttggttccca gtcaggtagt
       61 gccggcgcgg agtacggcga cggacgtgga gcgtcggccc gacagcccag gtgacgtcgg
      121 tggcattcct tcggcagtgg cacaaacagg aatcgagcgg gtgttgtcgc ccgcgcaccc
      181 ggagtccgtt ccggaacgcg tgcatcggga cagcccggtg ccctctcccg cggaggctga
      241 gccggaggtc aaccagcgtg gcgacagcgc gcggaaaccc cggaccaccc tgtcccgcat
      301 cgccggcagc tttaagctgc ctcccagcag cctgctgcac cgccccgacg aacagcaggc
      361 ggtggatgcg ggagagttga agctgctggc acaggtcctc acggaaaagt acgcagagtt
      421 cgatgtacac gggcaggtga cgcagatcaa tcccggtccg gtggtcacca ccttcgagtt
      481 caagccggaa gccggcatca agtacagccg aatcacgaac ctgaccgagg acttgtgcct
      541 ggccctcaag gcggaaagca ttctggtcga gcgcatggca ggcaagtcca cggtgggtat
      601 ccaggtgccg aaccgggagc gcgagatcat ctggctgcgg gaaaacctgg agtcggcgga
      661 attcatcggc tcgaagtcgc ggcttacgct ctctctgggc aaggacatca acgggcggat
      721 tgccgtggcc gatctagccg ggatgccaca tctgctgatc gctgggtcta cgggaacggg
      781 caagagcgtg gccatcaacg ccatgatcat gtccatcctg tacaaagcga cgccccacca
      841 ggtgaagctg atcctggtcg acccgaagcg gctggaactg ggcaactacg agggcgttcc
      901 ccacctgtac acaccgatca tcactgagcc caagctggcg gcgaacgctc tgcgcaacgc
      961 cgtgcgtgag atggagcggc gtctgaaatt gctggcggag aagggcgtgc gcaacatcga
     1021 ccagtacaac agactgtttg aagacgaaga gacgctcagc ctgttcggcg atgatccgga
     1081 ggagcggccc atcccttaca tcgtgatcat catcgacgag ttggccgacc tgatgatgct
     1141 cgactcgggg aacgtggaag aatcggtcac gcggctggcg cagatggcgc gagccgtggg
     1201 catccacctg gtgctggcca cgcagcggcc gtcggtggac gtgatcaccg gactgatcaa
     1261 ggccaatttt cccgcccgca tttccttccg ggtggcgacc aaggtcgatt cgcgaaccat
     1321 cctggatgcc aacggagccg aggccctgct gggacgcggc gacatgctct accttcccgc
     1381 cgggtcggca cgcgtccatc gcctgcatgc ccccttggtc accgagaagg aaatcgcggc
     1441 ggtggtggaa ttctggcgct cgcagggcga tgctgattac caggaagcgt tcctcgaagc
     1501 gcccccggaa gagcgggagg ccggcgagag agcggctgga gagttgggag aagacgagaa
     1561 cgacccgctg tttgcggatg cggtgcgcct ggtggtggag ttcggcaagg catctacttc
     1621 tctgctgcaa cgccgcctgc gcatcggcta cggtcgggcg gcgcacctga tcgatctgat
     1681 ggaacgcgac ggcatcgtag gcgccgccga cggccccaag ccgcgcgagg tcctgaaacg
     1741 gccggattgg atctcggaag tggaagagtc gatgcgctaa ttacttccag tgcgagcgga
     1801 aagcgagtgc aggagttcac gcggcgactc tgagagtcgc cctttctgtg tgcagacgat
     1861 gcgcaccatg ccggtcgcct ggtgccgtgg ttacagccga ggtgtgagca gagaaaaccc
     1921 cgagttccgc ttatgagccg tagcaccgat cccctgctcg ccctgcgccg ctactggggc
     1981 tatgactcgt ttcgcccact ccagcagcgc atcatccaaa gtttgctgag cgggcgagac
     2041 gcctgcgtgg tgatgcccac cggaggcggg aagtctctct gttaccagtt gccggccgtc
     2101 gtactggaag gcacggtcgt ggtgatctcg ccgctgatcg ccctgatgca ggaccaggtg
     2161 gcccagttgc gccagatggg gattcccgcc gctgggctca acagcatgct ggcggggaac
     2221 cagcagttcg aggtcatgca ggcggcgcgc gaaggcgctt accggttact gtacctgtcc
     2281 ccggagcggc tggcgcggcc ggacacgacg gagtggctgc ggcaggtccc ggtttccttc
     2341 ttcgccatcg acgaggccca ctgcatctcc gagtggggcc atgaatttcg gccggaatac
     2401 cgccagctga gccggctgcg gaccgagttt ccggagcggc cgatcgccgc cttcaccgcc
     2461 agcgccacgc gccgggtgcg gcacgacatc ctcgagcagc tgcagttgcg tgatccccac
     2521 aaatatattg ccagcttcca ccgccggaac ctgcgctacg tcgccaggca gagcgacgcg
     2581 gtctcgcagc cgcagctgct ggtccgagcg ctccggcagc tgggggacgg caacgcgatc
     2641 gtctactcgc cgaccattgc ccgggtggag cggaccgtcg ccatgctcga agaggaaggc
     2701 attccctcgg tcgcctacca cggcaggatg gagacagagg agcggcgccg caatcaggag
     2761 cgctggatgt cggatgaagt gcgagtgctg gtgggcacga tcgctttcgg tctcggcatc
     2821 aacaaagctg gcgtgcgtgc cgtcatccat ctcgcgctgc cgaagtcgct ggaacagtac
     2881 tatcaggagg ccggacgggc cggccgggat ggccggccgg cggactgcat ccttttatgg
     2941 cagaagcgag acaccgccct gctggcccac ttcatcgggc agatggaaga tcctgcggaa
     3001 aaggagcggg cctggcagcg ctaccgcgac attcgggatt tcgccgaatc gcattcctgc
     3061 cgccatcggc aagtgtgccg acactttggc gagacgccca agtgggcctc ctgcggggcg
     3121 tgcgacgcct gcggctccga gccggaatgg ctgaagcggc cggtcacgga ggagctcccg
     3181 cgacggaccc ggtggaagcc gccatcaccg cccgccgcgc ccggcaccac cggcgctccc
     3241 gggccaaggg ctgcggagat cgatcaggca ctttgggatt acctgcgaga atggcgtcgt
     3301 tccacggcga ggcagcaaag gatcccggct tatatcgtgc tgcatgatcg ttcgctcgag
     3361 gaattgtgcc gggtccgtcc gcgaaccttg cgggacctca ccggcgtccc tggcttcggg
     3421 aagcgaaagg tggagcttta tggaaggcag attctggacg cgctg
//
LOCUS       DASPUL010000465         3966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1453748, whole genome shotgun sequence.
ACCESSION   DASPUL010000465 DASPUL010000000
VERSION     DASPUL010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453748"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VEJ00_11080"
     CDS             <1..252
                     /locus_tag="VEJ00_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01751.1"
                     /translation="CAIVANDIPVFREIWGDAALYFRTNDAASLAESIRLLNEDRAMR
                     RAYAELAYTRARERFTTKRMIEDYLQLYRSVMAADSLAA"
     gene            451..681
                     /locus_tag="VEJ00_11085"
     CDS             451..681
                     /locus_tag="VEJ00_11085"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HXX01752.1"
                     /translation="MAQVKITIRPNGPYRVEAAEGVVELVDVNGTHYDLTGKPAFSLC
                     RCGGSVNKPFCDGTHSKIGFQGAELAVKKAEG"
     gene            727..1248
                     /locus_tag="VEJ00_11090"
     CDS             727..1248
                     /locus_tag="VEJ00_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01753.1"
                     /translation="MRRTFRLCFVAFALLSAATAQQTKPAPDWKALAWLVGDWSGAGN
                     GAPGQGSGGFSFQFDLQGKVLVRKSFAEYPAAGEKPAYRHDDLMVVYPEAEGFKAVYF
                     DNEEHMIRYSIDTSADGTVTFVSDGPASAPRFRLAYHKTGETTLTGKFEVAPPDKPFS
                     TYLEWNAQKKSAK"
     gene            complement(1283..2650)
                     /locus_tag="VEJ00_11095"
     CDS             complement(1283..2650)
                     /locus_tag="VEJ00_11095"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXX01754.1"
                     /translation="MQHEAAQRGEADDNVRQRVMPTPWVRRESTITLTHVLFGANLAV
                     FLAMQLAGGPVMDFDGHELAHWGANVGPYTLSGDWWRLVTYMFLHGGVMHIAFNMWCL
                     WDLGAMCESLYGRWTYATIYLITGIAGGLASVAWNPGVLSVGASGAIFGLAGALVASF
                     YLGEFSLPRVALSGTLRSLAFFIGFNVLFGSMFPGIDNACHIGGLLSGLMLGALIARL
                     APEHDRPLKRAGVVAVVALAVAASGVAVRQWRSAPFRMSRALEAASQNTPDRVVAQLK
                     EIVRQQPNSVQAHFALAQTYFGEEKFSEAEAEFKRVLQLQPQHAAARFELGMVYVSER
                     RLDDANSAFAQLVAQDANDPNGHLGMGLVLAAEGNQQEAIGEYKNAIRLNPQISGAQY
                     ELGRSYMKLKMYDEAIAAYLKERENTGDDPDIETALADAYQAKGMTQQAREASDKAAE
                     LKRQR"
     gene            complement(2964..>3966)
                     /locus_tag="VEJ00_11100"
     CDS             complement(2964..>3966)
                     /locus_tag="VEJ00_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXX01755.1"
                     /translation="NDGTLEQLIEKLIERMEQENYISIDQPHDPAKQSVAPGQTGKPQ
                     SQVKFEITDKSLDFLGFKALRDLLGSLGKSSFGRHDTRDLATGVEASGASRRYEFGDT
                     LNLDITATLSSALQREGLKLPLNIEYSDLQVHQCEYQSSCATVLMLDCSHSMILYGED
                     RFTPAKKVAMALSHLIRTQYPGDSLSLVLFHDSAEEVPLSQLARVKVGPYYTNTREGL
                     RLAQRILQRQRKDMKQIVMITDGKPSALTLEDGRIYKNAFGLDPLVVSETLEEVSKCK
                     RAGVLINTFMLASDYGLVQFVQKVTEMCRGKAYFTTPYTLGQYLLMDYMSRKTKTIH"
BASE COUNT          763 a         1148 c         1210 g          845 t
ORIGIN      
        1 tgcgccattg tggcgaacga cattcccgtc ttccgtgaga tctggggaga cgccgctttg
       61 tacttccgca ccaatgacgc cgccagcctg gccgaaagca ttcgcctcct caacgaggat
      121 cgcgccatgc gccgtgccta tgccgaactg gcttacaccc gcgcccgcga acgcttcacc
      181 accaaacgca tgatcgagga ttatctgcag ttgtatcgca gtgtgatggc ggcggactca
      241 ctcgcagcgt gacggcgccc ggcaacgctt ccctcttggt cgatgtttac cacgaagcac
      301 gcgaagttac acgaagggtc ttcgtggacc tctgttgtcc ctctggttcg cgaatcggcc
      361 attttcgaag aatcgcctgg caagacacgt ttccgcactt ggtgatatct tcagtttttc
      421 gagatcccaa caagtttgga ggatgcattc atggcacagg tcaagattac gatccggccc
      481 aacggcccgt atcgagtgga agctgcggaa ggggtggtcg aactggtgga tgtaaacggg
      541 acccactacg atcttaccgg caaacccgcg ttttctctct gccgctgcgg cgggtcggtg
      601 aacaagccct tctgcgatgg aacgcacagc aagattggct ttcagggcgc cgaactggcg
      661 gtgaaaaagg ccgaaggctg atcgacgccc ttttgagtct tcaggtctga ttgccgggag
      721 gtcggcatgc ggagaacgtt caggctctgc tttgtagctt tcgccctgct gtcagccgca
      781 actgcccagc agacgaaacc tgcgcccgac tggaaagcac tggcgtggct cgtcggcgac
      841 tggagcggag caggaaacgg tgctccgggt caaggttccg gcggcttttc cttccagttc
      901 gaccttcagg gcaaggtcct ggtgcgcaag agtttcgcag agtatcccgc agctggcgag
      961 aagcccgcct atcgccacga tgacctgatg gtggtatatc cggaagcgga gggtttcaag
     1021 gcagtctatt tcgacaacga ggaacacatg atccgctact cgatcgatac ttccgctgac
     1081 ggtactgtga cttttgtaag cgacggtcct gccagtgccc cacgcttccg cctggcgtat
     1141 cacaagaccg gcgagacgac attgactggg aagtttgagg ttgctccgcc cgacaagccc
     1201 ttctcgacat acctggagtg gaacgcgcag aaaaagtcgg ccaagtaggt tccggcgcct
     1261 gcttcagtgg gtgagcggca atctatcttt ggcgcttcag ttccgccgcc ttgtcgctcg
     1321 cttcccgggc ctgttgcgtc atccccttag cctggtaggc atcggccaag gcagtttcta
     1381 tatcaggatc gtcgcccgta ttttcccgct ccttgagata cgcagcgatc gcctcgtcgt
     1441 acatcttcag cttcatgtag gaacgaccta gctcgtattg ggctcccgag atttgtgggt
     1501 tgagccggat ggcgttcttg tactctccaa tagcctcctg ctgattgcct tccgcggcga
     1561 gcaccagccc catgccgaga tggccgttgg gatcgttggc gtcctgggcc acaagttgtg
     1621 caaaggcgct gttggcatcg tcgagacgcc tctcgctcac atagaccatt ccaagttcga
     1681 agcgggcggc tgcgtgctga ggctgaagct gcagcactct tttgaactca gcttcagcct
     1741 cggagaattt ctcctccccg aagtaagtct gggcgagcgc gaagtgtgcc tgcaccgagt
     1801 tcggctgctg gcgcacgatt tccttcagct gagcgaccac gcgatccggg gtgttttggg
     1861 atgctgcttc gagagcccgg ctcatgcgga agggagcgct gcgccactgg cgcacggcga
     1921 ccccgctcgc ggccacagcg agcgccacga cggcgactac accggcacgc ttgagcggcc
     1981 ggtcgtgttc cggcgcgaga cgcgcgatga gcgccccgag catcaggccg ctgaggagtc
     2041 cgccgatgtg gcaggcattg tcgatgccgg gaaacatgct tccaaacagg acattgaagc
     2101 cgatgaagaa ggcgaggctt cgtagcgttc cactgagggc gactctgggt agagagaatt
     2161 cgcccagata gaaagatgca accagagcac ctgccaggcc gaagatcgcg cctgatgcgc
     2221 cgacgcttaa cacccccggg ttccaggcaa cactcgctaa accgccggca atgcctgtga
     2281 tcagatagat cgtggcgtaa gtccagcgac cgtagaggga ttcgcacatc gcgcctaaat
     2341 cccacaggca ccacatgttg aacgcaatgt gcattacccc accgtgcaga aacatgtagg
     2401 tgacgagccg ccaccagtcg ccggacaagg tgtaggggcc gacgttggct ccccaatgag
     2461 ccagctcatg cccgtcgaaa tccataacgg gaccaccggc cagctgcatg gcaaggaaca
     2521 ccgccagatt cgcgccgaag agcacgtgag tgagcgtgat cgtcgattcg cgccgcaccc
     2581 atggtgtggg catgacgcgc tgcctcacgt tgtcgtccgc ctcgccgcgc tgcgcagctt
     2641 catgctgcac gcaccacggg catatcctcc gaaaggaaaa tggaggcagc ttgcgtccgc
     2701 attgggtaca gttggccatg ggttagaacg gatcactgtc attctgagcg aagcgaagaa
     2761 tcccttgcaa gtcggtggtg gcgcaggcgc cgcagggaat tttcgggttg atgtcgtgtt
     2821 tgagttggtg ggcgccgata cggtcgcgcc cgtggcgtgg tgcgggggtg gcgatggcgg
     2881 aaatgccttg agacgaccgt ggtggcgcca tctggcatgg gattcttcgc ttcgctcaga
     2941 atgacagtga tggtttgaag agcctaatga atagtttttg tcttccgcga catgtagtcc
     3001 atcagcaggt actggcccag cgtgtagggt gtggtgaagt aggctttgcc gcggcacatc
     3061 tcggtcactt tttgcacgaa ctggaccagg ccatagtccg aggccagcat gaaggtgttg
     3121 atcagcaccc cggcgcgctt gcattttgaa acttcttcca gcgtctcgct gacgacgagc
     3181 ggatcgagcc cgaaggcatt tttgtaaatg cggccatctt cgagcgtgag cgccgaaggc
     3241 ttgccgtcgg tgatcatcac gatctgcttc atgtcttttc tttgtcgttg caaaatcctt
     3301 tgcgccagcc gcaggccctc gcgcgtgttc gtgtagtaag gcccgacttt gacgcgcgcc
     3361 agttgcgaca gcggcacttc ttcggccgag tcgtgaaaca gcacgagcga aagcgaatcg
     3421 ccgggatatt gcgtgcgaat cagatgcgac agggccatcg cgactttctt cgctggcgtg
     3481 aagcggtctt cgccgtagag aatcatcgag tgcgagcagt cgagcatcag caccgtggcg
     3541 cacgacgact ggtactcgca ctggtggacc tgcaggtcgg agtactcgat gttcagcggc
     3601 agcttgagac cttcgcgctg cagggcgctg gataatgttg ccgtgatgtc caggttgagc
     3661 gtgtctccga actcgtagcg cctcgatgct ccgctggcct cgacgccggt agcgagatcg
     3721 cgagtatcgt ggcgtccaaa gctcgacttt cccagcgaac ccagcaggtc gcgcagcgcc
     3781 ttgaagccga ggaagtctag gctcttgtcg gtgatctcga atttcacctg cgattgcggc
     3841 ttgccggtct ggccgggagc gactgactgc ttcgcaggat cgtgcggctg gtcgatggag
     3901 atgtagttct cctgctccat acgctcgatc agcttctcga tgagttgttc gagggtcccg
     3961 tcattg
//
LOCUS       DASPUL010000466         8914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_637400, whole genome shotgun sequence.
ACCESSION   DASPUL010000466 DASPUL010000000
VERSION     DASPUL010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637400"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VEJ00_11105"
     CDS             complement(<1..378)
                     /locus_tag="VEJ00_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01756.1"
                     /translation="MHRLIVVALLAAVTPWMSAQRAPAQHPAPATQPVRHTRPFFYPS
                     LFVPDVFYPDIFSTEEYSVASQPPVILLQAPPASNPAPEPVPSPAEPLVIELQGDRYI
                     RLSGTDTSQPGMVEASPVPLRRPE"
     gene            complement(381..1142)
                     /locus_tag="VEJ00_11110"
     CDS             complement(381..1142)
                     /locus_tag="VEJ00_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01757.1"
                     /translation="MNRRLRYHVAVRRSTPASVALFAAVALATVFLPAACAQISTGQA
                     STSAPASGMGSHAGSFVPGVAPHTSNIVVVPPTGPVVPRTGTVPLPSLPNRGVAHTHH
                     PRHDHDFVGFGLYPYAYPVPVPYQPDEAQGAGDDSEDDADYQGGPTVFDRRGSGADSY
                     VPPVKDVPSPHSDQVADAFPPAPESPQEPTVLVFKDGEKLEVGNYAIVGATLFDLSPG
                     HHRKIMLADLDLDATQKLNDDRGVIFQLPASSQAN"
     gene            1321..2121
                     /locus_tag="VEJ00_11115"
     CDS             1321..2121
                     /locus_tag="VEJ00_11115"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HXX01758.1"
                     /translation="MKSAFRYALLALVLVIVAMLSALTAMRFAIHGQEVAVPPLVGMA
                     PADAERAVTGLGLQMSIERQYYSPQIPEGKIMSQLPPPGTKVRRGWQVRVAQSLGPTR
                     VAIPDVTGQSEHAAELNIRRRGLDVASMAEVEAPGIPVDQVLAQSPPANATQVLAPKI
                     SLLVSANAEPPAFVMPSFVGQPLGSASRTLQDEGFRLGNVSVAAPVVAPTDSGTTSMD
                     QGSAQPVPAPPAPPAPPAQPSPASIIVSQAPSAGQRVVAGTAVNFEVR"
     gene            complement(2227..2778)
                     /locus_tag="VEJ00_11120"
     CDS             complement(2227..2778)
                     /locus_tag="VEJ00_11120"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF014870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HXX01759.1"
                     /translation="METLHFAIHVSPVGPLFLAASERGLVALEFDARLPGQQTIRPNP
                     RDLRSESQRAEKHSFRFEPSPRALRDYVHELEEYFSGKRREFTFPLDLRGTDFQLACW
                     RALLAIPYGETRTYADIAHAVGKPHAFRAVGMANNRNPVAIVVPCHRVIASDGTLCGY
                     GGGLDIKRKLLELEGALSGTLAA"
     gene            3012..3431
                     /locus_tag="VEJ00_11125"
     CDS             3012..3431
                     /locus_tag="VEJ00_11125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HXX01760.1"
                     /translation="MKKALLILAAVVLAPALVVSLVVLAAGDWRTIGTTGAGDTVSVS
                     SIRVLKSNQRVALVRVEFKEPATLPQGGPFVEMRARVRFNCTNGGAAPTSEWFYTRDH
                     SGRFVVSKKATRDDQFGKDPEGGFADMVSKSVCGQGK"
     gene            3528..3920
                     /locus_tag="VEJ00_11130"
     CDS             3528..3920
                     /locus_tag="VEJ00_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01761.1"
                     /translation="MDSLRLYGLVTAAAVALLGAYALLRRRPKTADDLERERRAWLEG
                     TGRITDGTVIDVQELAAQQKRRAAVMLIYKYDVAGVSYECSQDVTYLRHWINLHSCRL
                     GLHTSVKYDPQNPGNSLVVSENWMGLRQ"
     gene            complement(4157..5791)
                     /locus_tag="VEJ00_11135"
     CDS             complement(4157..5791)
                     /locus_tag="VEJ00_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXX01762.1"
                     /translation="MENSANPETRRFGRYEIDSEIGRGAMGIVYKARDPQIDRWVALK
                     TIPLHSQQTEMVEEFRKRFLNEAQAVGRLHHPGIVAVYDVGEDEQHHDPYIVLEYVAG
                     EALNKLLSRDRRLPLARAMQLAEEIAEALDYAHLQGVVHRDIKPGNILLTPEGHAKLA
                     DFGIAKMDLSHTTLPGKVLGTPAYMAPEQLEGGKTDGRSDLFSLGVILYAMVTGRSPF
                     RGDNSSAVCHKVVNLNPPPPSAVNVDLPKELDPVVARALAKKPSKRYERGAEFAEAIR
                     QLRERVAPALVPVSPEKVPGAAQQNVAPKKPVAALPRRPATGLAYAVKVVRSAILQAP
                     LKDLALGFATITMLLIVGIQSHLLFHAHEQEVAAADTPAVRPSAATAASAGNAPASAA
                     SAKVKNSSLASSAVAGKATVKSASAKPLAGASPVPTAAPQVIVPSSTLDLAVQHQFKD
                     ATLYVWVDDQLVLKRALHGGTQKRLVVFNGVRGVDSESVQVLAGKHTLRLRALTADQT
                     VDLSKSVTAEFIGGGEKALQITFDKHNSAMKLNWRQ"
     gene            complement(6342..7253)
                     /locus_tag="VEJ00_11140"
     CDS             complement(6342..7253)
                     /locus_tag="VEJ00_11140"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXX01763.1"
                     /translation="MQKRFYILFVARGDDGQLRKISIPVHYLYVFVVGAAIGFLSLTG
                     IASSYTRMLLKVSRYNQLKVEKDQLKDNYSRLEQVAKERDVQVASLGSIAGEVSALYG
                     LKAQPTLVTATADQIQAAEVSSSLDQLHALRTSALTGATMVGLTMGLTRNATTADWLK
                     ANSAPNLWPVEGQVTGSFGERIDPFNGEGAFHSGVDISSAYGHPIVAPADGVVTFTDQ
                     MGGYGKAIIIDHSNGISTRYGHLSGFAVTAGQAVHRGDVIGYVGASGRSTGPHLHYEV
                     RINDTPVNPYKYLRITVAHSGGFAAGS"
     gene            complement(7772..8167)
                     /locus_tag="VEJ00_11145"
     CDS             complement(7772..8167)
                     /locus_tag="VEJ00_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01764.1"
                     /translation="MKNHVSNTVFVLLVLFLAVSLHAQDKIATPEYWTAVGATAGMTY
                     FDAATTLKVAGTRNCTEEGWTPWLYGKHPTTGRTVAAMSGEVVAGAVLSYEIKKHVRG
                     KLGRMWAAPLGYLAGVHARGAIHNLTVCG"
     gene            8535..>8914
                     /locus_tag="VEJ00_11150"
     CDS             8535..>8914
                     /locus_tag="VEJ00_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HXX01765.1"
                     /translation="MLRFLPGKQSGNADILVTTDFTLEGIHFRRDWHPAESVGHRCLA
                     RGLSDIAAMGGDPVAAFLSLALPRDLPQAWVGRFARSLISLAEKHGATLAGGDTAESP
                     DGVLADIVVVGTVPKGKAALRSGAR"
BASE COUNT         1684 a         2564 c         2966 g         1700 t
ORIGIN      
        1 ttccggacgg cggagcggga cggggctcgc ctcgaccatg ccgggttgcg aagtatcagt
       61 gccgctcagc cgaatgtagc ggtccccctg caattcgatc acgagcggct cggccggcga
      121 tggaacgggc tcgggagccg ggtttgacgc gggtggggcc tgcagaagaa ttacaggcgg
      181 ttgagaagcg acggaatatt cttcggtgga gaaaatatcc gggtagaaca cgtcgggcac
      241 gaagagggac ggatagaaaa aaggccgagt atggcgaaca ggctgcgtag cgggcgccgg
      301 gtgctgggcg ggcgctcgct gagcgctcat ccatggcgtg accgcagcca gcagcgcgac
      361 gacgatgagc ctatgcatcg ctagtttgcc tgtgacgatg ccggcagctg gaagatcacg
      421 ccgcggtcgt cgttcagttt ctgcgtggca tcgagatcga gatcggcgag catgatcttg
      481 cggtgatgcc cgggggagag atcaaaaagc gtcgcaccca cgatggcgta gttccccact
      541 tccagcttct cgccgtcctt gaacacgagc acggttggct cttgcggaga ctcaggggca
      601 ggaggaaagg cgtctgcgac ctggtcggag tgaggactgg gcacgtcttt caccggcggc
      661 acgtaggagt cggcgcccga tccgcggcga tcgaaaacgg tcgggccgcc ctggtagtcg
      721 gcgtcgtctt cggagtcgtc gccggcgcct tgcgcctcgt ccggctgata gggcacgggc
      781 accggatagg cgtagggata aaggccgaat cctacaaaat catgatcatg acggggatga
      841 tgagtgtgcg ccacaccgcg attcggaagc gaaggcagcg ggacagtgcc ggtccggggg
      901 acgacggggc cggttggagg tacgaccacg atattagacg tatgcggcgc gacacctggc
      961 acgaagcttc cagcgtggct gcccattccc gatgcggggg ccgaagtcga agcctgacca
     1021 gtggagattt gtgcgcaagc ggcgggcagg aacaccgttg cgagcgcaac cgcggcgaac
     1081 agcgcgaccg aagctggagt cgaacgccgc acggcaacat gataacgcaa tcgacgattc
     1141 atggtgcacc ccaacgctag aacgctctcc ttaggtttga ttgcatccgc aggtggccgg
     1201 atgcccgatt tgtggcgatc ccggccagcg tgacacgggt tgtatgttat tgaaattacg
     1261 gctgtccaaa gggtccgact ataatcccga gagacgacga agcctttcgc gacgggaaat
     1321 atgaaatctg ccttccgata tgccctgctg gcgctggtac ttgtaattgt cgccatgctg
     1381 tcggcactga cggcgatgcg ctttgccatc cacggacagg aggtggcagt gccgccgctg
     1441 gtggggatgg ctccggcgga tgccgagcgc gcggtcacag ggctgggatt gcaaatgtcg
     1501 atcgaacgcc agtactacag cccgcagatt cctgaaggaa agatcatgtc gcaactgccg
     1561 ccgccgggaa cgaaagtgcg gcggggctgg caggtgcggg tggcgcagag ccttgggccg
     1621 acgcgggtgg cgattccgga tgtgaccggg caaagcgagc acgcggcgga attgaacatc
     1681 cggcggcgcg ggctggacgt ggcttccatg gcggaagtgg aggcgccggg gattccggtg
     1741 gatcaggtgc tggcgcagag tcctccggcg aacgcgaccc aggtgctggc tccgaagatc
     1801 agtttgctgg tttcggcgaa tgcagagccg ccggcgtttg tgatgccgag ttttgtcggg
     1861 cagccgctgg ggagcgcgag caggacgctg caggatgagg gatttcgact ggggaacgtg
     1921 agcgtggcgg cgcctgttgt cgcacctacg gattccggga cgacttccat ggatcaggga
     1981 agtgctcagc cagttcctgc tccaccggcg ccgcctgcgc cgccggcaca gccatcgccc
     2041 gccagcatca tcgtgtcgca ggccccgtcg gcgggacaga gagtcgtggc cggaactgcg
     2101 gtgaattttg aggtgcggta gtgaaggctt tcttcgaccg ggatggggta tggtgcagga
     2161 cgggtcgtca acgagtgtaa cggcaattac actcatcgac cgagttctca tccatcacgc
     2221 catgatttac gcggccagcg taccgctgag agcgccttca agctcgagca gcttgcgctt
     2281 gatgtcgagc ccgccaccgt agccgcagag ggtgccgtca gaagcgatga cgcgatggca
     2341 cgggaccacg atcgccaccg gattgcgatt gttggccatg ccgacggcgc gaaaggcgtg
     2401 cggcttgccg acggcgtgcg cgatatcggc gtaggtgcgg gtctcgccgt agggaatcgc
     2461 cagcagggca cgccagcagg cgagctgaaa gtcggttccg cgcagatcga gcggaaaggt
     2521 gaactcgcgg cgcttgccgg aaaagtactc ttccagctcg tggacgtagt cccgcaaggc
     2581 acggggcgag ggctcgaagc ggaaactgtg cttttcggcc cgctggctct cggagcgcag
     2641 atcgcgcggg ttggggcgga tcgtctgctg acccggcagg cgggcatcga actccagcgc
     2701 caccaggccg cgttccgacg ccgccaggaa cagcggcccc acgggggaga catgaatcgc
     2761 aaagtgcagt gtttccatag atttgtggat ccgctccacg agagcccggt atcagctcag
     2821 ccgggcactc agccgggcac aaccaacaca ttatagcgag ggacaaacgc tcggcgctcc
     2881 gtcctcgggc catattattg acagaatcgc tattttgccc cacagtgtgt gctgtctctg
     2941 gttgcccgaa acgttgccct gaaatgatgc ctcgcgacgt tgctctagaa gggctgcctg
     3001 ggaagctcgg gttgaaaaaa gctctcctca ttctcgccgc cgtcgtgctg gccccggcac
     3061 tggtggtcag cctggtagtt ctggccgccg gggactggcg caccatcggc accaccggag
     3121 cgggcgacac cgtctcggtg agctctattc gcgtgctgaa gagcaatcag cgtgtagccc
     3181 tggtgcgcgt ggagttcaag gaaccggcga cgcttcccca gggcggtcct tttgttgaga
     3241 tgcgggcgcg ggttcggttc aactgcacga acggcggagc tgctcctacc tccgaatggt
     3301 tctacactcg cgaccacagc ggacgcttcg tggtcagcaa gaaagccacc cgcgacgacc
     3361 agttcggcaa agatccggag ggcggattcg ccgacatggt cagcaagagc gtgtgcgggc
     3421 agggcaagta agtcgggact ctcgccgcat ggcacctcgg taacctccaa actacttcct
     3481 ccaggcttgc tttgcgctta tgctgatagc tctaacccac ctgcattatg gattcactgc
     3541 gactttatgg tttggtcacg gcagcggcag tcgcgctgct gggcgcgtat gcgctgctgc
     3601 gccgccggcc gaagacggcc gacgatctgg agcgcgagcg ccgcgcctgg ctcgaaggca
     3661 cgggccgcat cacagacggc accgtcattg atgtgcagga actcgctgcg cagcaaaagc
     3721 ggcgggccgc cgttatgctg atctacaagt acgatgtcgc gggcgtatcg tatgaatgct
     3781 cgcaggacgt gacctacctg cggcactgga tcaacttgca ctcctgccgg ctggggctgc
     3841 acacatcggt gaagtacgat ccgcagaatc cggggaattc gctggtggtg tcggagaact
     3901 ggatggggct gcggcagtag tggagaatta cgaggaagac gaccactggg gctaaagccc
     3961 tcgatcactt ggcacgctta acgcggccct aaagggccgc tcttccacga aaactcgaac
     4021 gctcgttctt ccacgcagac tcgaacgctc gttcttccac gccagactcg aacgctcgtt
     4081 ctttcacgcg ggttcggacg ctcgcgcttc cacggtccct ggggctctcg tcccacgatc
     4141 aacaatcaac aatttcttac tgccgccagt tcagcttcat ggcgctattg tgcttgtcga
     4201 aggtgatctg cagtgccttc tcgcctccgc cgatgaattc ggcggtgacg ctctttgaga
     4261 gatcgaccgt ctgatcggcg gtcagggcgc ggagccgcag cgtgtgtttt ccggcgagaa
     4321 cctgcacgct ctcggaatcg acgccgcgca cgccgttgaa caccaccagc cgcttctgcg
     4381 tgccgccgtg cagggcgcgc ttcaacacca gctgatcatc gacccagacg tacagggttg
     4441 cgtccttgaa ctggtgttgc acggcgaggt ccagcgtgga agatggaacg atgacctgcg
     4501 gcgcagccgt cggcaccggg ctggcaccgg caagcggctt ggccgatgcg ctcttcaccg
     4561 ttgccttgcc tgccaccgcg gaactggcca gcgaactgtt ctttaccttt gcgctggccg
     4621 ccgaggcggg cgcgttcccg gcggaagctg cagtcgctgc gctggggcga accgcaggag
     4681 tgtcagcggc ggcgacttcc tgttcatggg cgtgaaacag cagatgtgac tggatgccca
     4741 cgatcagaag catggtgatg gtggcaaagc cgagagccag atctttgagc ggagcctgga
     4801 ggatcgcgga gcggaccact ttcaccgcgt aggccagccc ggttgccgga cggcgcggaa
     4861 gcgcggcgac gggtttcttc ggtgcaacgt tctgctgcgc agcccccggc actttctccg
     4921 gtgaaaccgg aaccaaagca ggggcaacgc gctcgcgcag ctggcggata gcctcggcaa
     4981 attcggcgcc acgctcgtag cgcttgcttg gcttcttggc cagggcgcgc gccaccaccg
     5041 ggtccagttc cttcggaaga tcaacattca ctgcgctggg cggcggtggg ttcaggttca
     5101 ccactttgtg acagaccgcc gaggagttgt cgccgcgaaa cggggacctc ccggtgacca
     5161 tcgcgtagag gatcaccccg agcgaaaaca ggtcagagcg gccgtcggtc tttccccctt
     5221 ccagttgctc gggcgccatg taagccggcg tgcccagcac tttccctgga agcgtcgtgt
     5281 gggacaggtc cattttggcg atgccgaagt cagcgagctt ggcgtggcct tcgggtgtca
     5341 acaggatgtt gcccggtttg atatcgcgat ggaccacgcc ctgcaggtgg gcgtagtcga
     5401 gggcttcggc aatctcttcg gcgagttgca tggcgcgcgc gagcggaagt ctgcggtctc
     5461 gcgacaggag cttgttgagc gcttctccgg cgacgtactc gagcacgatg tagggatcgt
     5521 ggtgctgctc atcttcgcct acgtcgtaga cggcgacgat gccgggatgg tgcaggcggc
     5581 ccacggcctg ggcttcattc aggaagcgct tgcggaattc ttccaccatt tcggtctgct
     5641 gggagtgcag cgggatggtc ttgagggcaa cccagcggtc gatttgagga tcgcgagcct
     5701 tgtagacgat gcccatggcg ccgcggccga tctccgagtc gatctcgtaa cggccaaaac
     5761 gccgggtttc gggattggcg gaattctcca tccggggtcg tccgcgggcg aggcccgacc
     5821 tgccatgcga gccctgggga gggagttctc ttaatgttag cgcggcagga agaaggattt
     5881 gacgggttgt ttggggcgtg gagttaccgg ggtaataggg gatgtatgtg cagttggttc
     5941 gtggtgcgcg atcgcgcgga ggtagcaata tgcggcatgc ggcgagagag aatattccgt
     6001 gtcgtccctg cgggactcga ttgtccttgg gatgagcttt cccgccacct acgtggcggg
     6061 ccaaggaatg ccgccgctgc gcggctgggt cttgcggtag gagagcttcc gggcgaaggc
     6121 gaccaccggg gctaaagccc cagatttctt cgatcgcttg acgcggccct taagggccgc
     6181 tcttccacga tggtcgcgac gcctggatcg aactgaagcg gagatggccg gatcacgcag
     6241 agacaggcaa atttgccgac gggaaaggcg ctgccgagag ccaaagggtg agagctgaaa
     6301 gccgagtacc gggaagggag aggaggaagc cggaagcgct gttagctgcc ggcagcgaag
     6361 ccgccgctgt gggctaccgt gatgcgcaga tacttgtagg gattgactgg cgtgtcgttg
     6421 atgcgaactt cgtagtggag gtgggggccg gtggaacgtc cgctggcacc gacgtagccg
     6481 atcacgtcgc cgcgatgcac ggcctgtccg gcggtcacgg caaaaccgga gaggtgtccg
     6541 tagcgagtgc tgatgccgtt gctgtgatcg atgatgatgg ctttgccata gccgcccatc
     6601 tggtccgtga aagtcacgac tccgtcggcg ggagcgacaa tcggatgccc gtaggcgctg
     6661 ctgatatcca cgccgctgtg gaaggcgccc tcaccgttga acggatcgat acgctcgccg
     6721 aagctgcccg tcacctggcc ttcgaccggc cagagattcg gcgccgagtt ggccttgagc
     6781 cagtcggcgg ttgtggcgtt gcgggtcagg cccatggtca ggccgaccat ggtcgcgcct
     6841 gtcagcgccg aagttcgcag agcgtgtagt tgatcgaggg aggaactcac ctcggcagcc
     6901 tgaatctgat ctgcggttgc cgtcaccagc gtgggctggg ctttcaatcc gtagagcgcc
     6961 gaaacttcac cggcgatcga gcccagcgag gcgacttgca cgtcgcgctc tttggccacc
     7021 tgctccaggc gcgaatagtt gtccttgagc tggtcttttt ccactttcag ctggttgtag
     7081 cgggaaacct tgaggagcat ccgggtataa gagctggcga tgccggtgag gctaaggaat
     7141 ccgatcgcgg cgccgacgac gaacacgtac aggtaatgca cggggatgga gatcttgcgt
     7201 aactggccgt cgtccccgcg ggccacgaaa agaatgtaaa agcgtttctg caaatcactc
     7261 ctcggtttgt cgaaacttta aatatcaggt ccggaacttc aggcgacgtt ctgaggttgc
     7321 agagcgcctg ttcgggccac ctgattcttc gggcttcttc ccatcttggg aatggaaacg
     7381 actttcaaag ccctacacct accgcggacc tggctttgga accgggatag gtctgcaaga
     7441 caatttcttg cagcatctta gctcggccaa aggtctatgt gaatgactga taaacggtaa
     7501 caaactggat tccccctgtc aatgacgcgc aaggccaatg ccgggtgacc aaagtggacg
     7561 gtaatcgatg gtcaccacgg gacgtgttac ctccggttcg gcaagaacca gagcggtccc
     7621 agcatagcaa ccaccgggct ccgttcaggt tcggttatag ggcgggcacg ggacaggagc
     7681 ggcaggacgc cgatcgacca aacccggacg ctgagtcgta gaaaggtcat ggcagcgaag
     7741 gccgaggtgt ctcgaacggc atcggagtgc atcagccgca cacggtcagg ttatgaattg
     7801 ccccgcgagc atggacccct gccaagtagc cgagaggagc tgcccacatt ctgcccaact
     7861 tgccacgcac atgtttcttg atttcgtaag agagaacggc accagcgacc acttcgccgg
     7921 acatcgcagc gacggttcga cccgtggtgg ggtgcttgcc gtagagccac ggagtccacc
     7981 cttcctcggt gcaatttcgc gtcccggcaa ctttgagtgt ggtcgcagcg tcgaaatagg
     8041 tcatacctgc tgttgcacca accgccgtcc aatattcagg cgttgcaatc ttgtcttgag
     8101 catgaagact tactgcgaga aacaggacca ataacacgaa aacggtattg ctaacgtgat
     8161 tcttcatagg gtgccaaggc aacgcgtccg gcgggaaccg ggtcaacgaa tgaaaagaat
     8221 ttaggtacct ctcccttgca gcgttgccaa gccctcctga cttcgaccgc tagagtcttg
     8281 atttttctgt aagaaattct gctcaatgca gaacccgctg tctgtgattg ggattacata
     8341 gtggtccgtg ctcttctgtg ccctccgtag ttcaggctct taagccgccg atccctccgc
     8401 tatagtgata ggctgtgccg cttcccgaaa aggcgctgat tgcccggatt cgccgcatgg
     8461 ccgccccttc aaggcaagag cgcgcgggca ggagtgcccc cgccacatgc gtcggaatag
     8521 gagatgactg cgccgtgctg cgcttcttgc caggtaaaca atccgggaat gcggacattc
     8581 tggtcacgac cgacttcacg ctggaaggga ttcacttccg ccgcgactgg catccggcgg
     8641 agtcggttgg gcatcgctgc ctggcccgcg gcctgagcga tatcgccgcc atgggaggcg
     8701 accctgtagc tgctttcctt tcgctggccc tgcctcgcga cctgccgcag gcgtgggtcg
     8761 ggcgtttcgc ccgcagcctg atcagcctgg cggaaaagca tggcgccacg ttggccggag
     8821 gcgatacggc tgagtcgcct gatggagtcc tggccgacat cgttgttgtc gggaccgtgc
     8881 ccaaaggcaa ggctgcgctg cggtcgggag cgcg
//
LOCUS       DASPUL010000467         3134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1861826, whole genome shotgun sequence.
ACCESSION   DASPUL010000467 DASPUL010000000
VERSION     DASPUL010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1861826"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2292
                     /locus_tag="VEJ00_11155"
     CDS             <1..2292
                     /locus_tag="VEJ00_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HXX01766.1"
                     /translation="YEALREAFGLRKSDCLVPDLNCWMPALGASICAVEAGAKRTAVA
                     FHRVPSQIASAEEIPTTAPLSLNNVTLLNRAPAVALPQGAGCVDAYLGIDVGSVSTNL
                     VVLDTAGSVLKEIYVPTAGRPIEVVTAGLREIEQELGPRLTIRGVGTTGSGRELIGEL
                     TGADTVNDEITAHKTGALHVCRQLGMPPVDTIFEIGGQDSKFIRICNGVVVDFAMNEA
                     CAAGTGSFLEEQAEKLGIAIKEEFARLALSSRNPIRLGERCTVFMGRDVSNLLLKGAP
                     VADLCAGLAYSVALNYLNRVVRGRKIGDVIYFQGGTAFNDAVAAAFSIILRKPIVVPP
                     HNGVIGAIGMALIARDRMLASRENSRFRGFNLHQVKWISRDFVCRACSNYCDMKVFVI
                     DGEKSYWGDQCSDKFRKRSRTDRQPVIEDLLSRRDQWLVEALQPPREERKTVGIPRAM
                     FYYDRFPFWCAYLQKLGYNVAVSEPTDRCIAAKGEELAIAQPCFPVQVAHGHVQALLD
                     SGVDYVLLPNAVDAETPFMQAESKLCPWNQTLPFVVRAVQKLDPHLNGKLLCPTIHFR
                     QGYQHVRAELRTLADHLGQSRAASDLAVAEGYRAQREFAEHVQGAGQEMLRILRVRGE
                     PAIVLLGRPYNIYDRAVCCDIPRKLRTLYGINVLPLDFLPLDSEDVSEINDNMYWHSG
                     RLILSAARFARANPYLHLIYISNFKCGPDSYLKSFIEEASGKPSLVLQFDGHSNDAGY
                     ITRCEAFLDSTGFLRCNSFDIAA"
     gene            2265..>3134
                     /locus_tag="VEJ00_11160"
     CDS             2265..>3134
                     /locus_tag="VEJ00_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01767.1"
                     /translation="MQLFRHRCITRKVGEDHPLAGKKVYVPDMAEGSVEALCAAMRWM
                     GIDAHPTPPPDARTLELGGMNTSGDECFPAKVTTGDFLKIAQQPGFDAKRTVFFMGTT
                     DGPCRFGQYAPFLRKVLRDCGYGEIRVFSPHGEHGYSDFQDFDTAFVRTAWRGLVSAD
                     ILRKLLLQVRPYETQPGNADRAYRESVDDLCGTIEQSCSDTECQMGSIAASLLRAADR
                     FHSVPAQFDGRRPLIGIVGEIYCRLNSFSNQDLVRRLEAQGAECWMSDIAEWIAYTNM
                     EEVRNLRLAGRGYS"
BASE COUNT          617 a          891 c          985 g          641 t
ORIGIN      
        1 tacgaggcgc tgcgggaggc attcgggctc aggaaatcag attgcctggt gcccgatctg
       61 aattgctgga tgccggcact gggcgcaagc atctgtgctg tcgaagcagg tgcgaagcgc
      121 actgcggttg cgttccacag agttccgtcc cagattgcat cggcggagga aatcccgacc
      181 accgcgcctc tttcgctcaa caacgtaact ttgctgaacc gagctccggc ggtcgcgctg
      241 ccgcagggag cgggctgcgt cgatgcttac ctcggaattg acgtggggtc ggtgagcacg
      301 aatctggtgg tactggacac cgctggcagc gtgttgaaag aaatctatgt tcctaccgct
      361 ggacgcccga ttgaggtggt taccgcaggc ctgcgcgaga tcgagcagga actcgggccg
      421 cgtctcacca ttcgaggcgt aggcacgacc ggatccggca gagagctgat cggggaactt
      481 acgggcgccg acacggtgaa cgacgagatc acggcgcaca agacaggagc gctccacgtc
      541 tgccgtcagc tgggaatgcc gccggtggac accatcttcg agattggcgg gcaggattcc
      601 aaattcattc gcatttgtaa tggtgtagtg gttgatttcg ccatgaatga ggcgtgtgcg
      661 gcaggaaccg gctcgttcct ggaagagcag gcagagaagc tggggattgc gatcaaggaa
      721 gagttcgccc gcctcgctct gtcatcccgc aacccgatca ggctgggcga gcgctgcacg
      781 gtctttatgg gtcgagatgt cagcaatctg ctgctgaaag gtgcgccggt ggcggatttg
      841 tgcgccgggc tcgcctactc ggtagccctg aactatctca atcgggtcgt ccgcgggcga
      901 aagattggcg acgtaattta ttttcaggga ggtacagcct tcaacgacgc ggtcgctgcc
      961 gccttctcca taattttgag aaagcccatc gttgtgccgc cccacaacgg ggtgatcggg
     1021 gccatcggga tggcgctcat tgcgcgcgat cgcatgctgg cgagccggga gaacagccga
     1081 ttccgcggat tcaatctgca ccaggtgaaa tggatcagcc gcgacttcgt ttgccgtgcc
     1141 tgctccaact attgcgatat gaaagtgttt gtgattgacg gcgagaaaag ctattggggt
     1201 gaccagtgct ccgacaaatt tcgcaagcgg tcacgcactg accggcagcc ggtgattgaa
     1261 gacctgctgt cgcggcgcga tcaatggctc gtagaggcgc tgcagccgcc tcgggaggag
     1321 cggaaaacgg tcggcattcc ccgggccatg ttctattacg atcgcttccc cttctggtgt
     1381 gcctatttgc agaaactcgg gtacaacgtg gcggtttcgg aacccaccga ccgatgcatc
     1441 gcggcgaagg gcgaggagtt ggccattgct cagccctgtt ttccagtcca ggtggcgcac
     1501 ggccacgtgc aggcgctgct ggattcaggc gtggattacg tcttgctacc gaatgccgtc
     1561 gatgcagaga caccgttcat gcaggcggag tcgaaactgt gtccgtggaa ccagaccttg
     1621 ccgttcgtcg tgcgcgccgt gcaaaaacta gatccccatc tcaacggcaa gctcctctgc
     1681 ccgaccattc acttccgcca gggataccag catgtgcgcg cggagttgcg cacgctcgca
     1741 gaccatctgg ggcagagccg agcggccagc gatctcgccg tggccgaagg ctatcgggca
     1801 cagcgtgagt tcgccgagca tgtgcaaggt gcaggacagg agatgctgcg aatcctgaga
     1861 gtgaggggcg agccggcaat cgtgctcttg ggacggccgt acaacatcta tgaccgggct
     1921 gtgtgttgcg acattccccg caagctgcgg actttgtacg ggatcaacgt gctgcccctc
     1981 gattttctgc ccctagacag tgaagacgtg tcggagatca acgacaacat gtactggcac
     2041 tccgggcgat tgatcctgtc ggccgctcga tttgcccggg ccaatcccta cctgcatctg
     2101 atctacatct ccaacttcaa gtgcggccct gactcttatt tgaagtcgtt tatagaagaa
     2161 gccagcggca agcccagtct ggtgctgcag tttgacggcc actcgaatga cgctggctac
     2221 atcacccgtt gtgaagcctt tctcgacagc acggggtttt tgcgatgcaa ctctttcgac
     2281 atcgctgcat aactcgcaag gttggcgagg accatcccct ggcggggaag aaggtttatg
     2341 tccccgacat ggccgaggga agcgttgagg ctctgtgcgc ggcaatgcgt tggatgggca
     2401 tcgacgcgca tccgacaccg cctcctgatg cgcgcaccct ggagttgggc ggcatgaaca
     2461 ccagcggcga tgaatgcttc ccggccaaag ttactaccgg ggacttcctc aagatcgctc
     2521 agcagcccgg cttcgacgcg aagcgcaccg tcttttttat gggaacgacc gacggcccat
     2581 gccggttcgg acaatatgcg ccctttctgc gcaaggtgct gcgcgactgc ggctacggag
     2641 agatacgagt cttttctcct cacggagaac acggctacag cgacttccag gattttgaca
     2701 ccgctttcgt gcggacggcg tggcgtggcc tcgtgtcagc ggacatcctg cgcaaactct
     2761 tgctgcaggt ccggccctat gaaacgcaac ctggaaacgc ggaccgcgct taccgagagt
     2821 ccgtcgacga tttgtgcggg acaatcgagc agagttgttc cgacactgag tgccagatgg
     2881 gttccatcgc agcctcactg ctgcgggcgg cagatcgttt tcactcggtg ccagcgcagt
     2941 ttgacggcag gcgcccattg atcggaatcg tgggcgagat ttattgccgg ctgaatagct
     3001 tcagcaatca ggacctggtt cgccggctgg aggcccaggg tgcggagtgc tggatgagcg
     3061 acatcgccga atggatcgcc tacaccaaca tggaggaagt tcggaatctg cggctggcag
     3121 ggcgcggcta ctca
//
LOCUS       DASPUL010000468         3875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1732301, whole genome shotgun sequence.
ACCESSION   DASPUL010000468 DASPUL010000000
VERSION     DASPUL010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732301"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VEJ00_11165"
     CDS             <1..192
                     /locus_tag="VEJ00_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01768.1"
                     /translation="DLGENRFSDGRRRSPQPFTWEVVFDNSGNVLDQGWLANGLLQQR
                     MRHLVPQQPVPAPPSRPKS"
     gene            189..794
                     /locus_tag="VEJ00_11170"
     CDS             189..794
                     /locus_tag="VEJ00_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXX01769.1"
                     /translation="MRVLIFSDIHGDLRALDRILQQPADLYIAAGDLATFGKGLDRCG
                     EVLRPLGSRLWVLPGNHETHEDTRALCARFGFIDFHRQMRSVEGGTGCAQWAGLGYSN
                     ITPFHTPGEYSEEEIANALAAFDKVPGLYVVAHCPPRDTTLDEFAPGQHGGSSSLRAW
                     IKRAQPSYLFCGHIHEAAGLRERVGVTECINVGKQGYTVEI"
     gene            complement(791..1390)
                     /locus_tag="VEJ00_11175"
     CDS             complement(791..1390)
                     /locus_tag="VEJ00_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HXX01770.1"
                     /translation="MSDERGATRKRTSSQGRGGRGSAEPLLDRERTLAELKRFLDLAI
                     AEMHLAVGWDLSAPTPGAAGSEDADIAVTFRGTDDELLLERNAELLLALEHIAHRWLR
                     LDPRLHDRVRLDCGDYRATRLAELKLSARVAAQRVRETGQPHRFNPMSSRERRIIHLE
                     LNGAQGVRTVSEGLGDRRQLIIYPTGGRGEDASLKNHRR"
     gene            complement(1387..3126)
                     /gene="yidC"
                     /locus_tag="VEJ00_11180"
     CDS             complement(1387..3126)
                     /gene="yidC"
                     /locus_tag="VEJ00_11180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXX01771.1"
                     /translation="MSDQVRAILFVVLVVLITFVWLHFYKPPVTSPEKPAQTASQTVP
                     GAGQAQPGVEAALSATPERVSTPRAEAQAERIITIESPLYHVELSNRGAVVRSWTLKK
                     YFDDQKPPRPLDLVNENAAKELGWPFSLQLSDSELEADANAGLYEVQTAAASSEAGKT
                     SMVPANANAFNAPVRVSFHWSNGHLDVSKTLSFGSDYQMSLEASAALDGKPLASGVAW
                     RGGFGDRTVYRASQLVSVFYQQGGSLSTIQYKKLGVSGNSTQPFQQPGAMEFAGIEDQ
                     FFTAAFIPNGTVLYLSHWTQWQHLVANGQQTAEPMAEMAVGSAAPVPVKLRAYVGPKD
                     LALLSKVQPSLEGLVQFGWTGVIAKPMLFILQWLHRLIPNYGWAIVVLTLGLTMALLP
                     IRIWTFHSMRKMQVIAPEMKAIQDRYRKYSMTDPRRSKMNEEIMAMYAKHGLSPWSQV
                     GGCLPMLLQIPFLWAFYRMLAGAIELRHAPWILWIHDLSAKDPYYVLPIAMAVTTYLS
                     TKMMPTASTVDPAQQRMMALMPLMLAFIFFQLSSGLNLYYFTSNLVSVVQQLYLNRTQ
                     PLPTRSKFKKKKP"
     gene            complement(3132..3368)
                     /gene="yidD"
                     /locus_tag="VEJ00_11185"
     CDS             complement(3132..3368)
                     /gene="yidD"
                     /locus_tag="VEJ00_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXX01772.1"
                     /translation="MLFLLRCYIVFFSPFFGGACKFQPSCSNYAYEAIQLHGAWRGFV
                     LAMKRLLRCRPFTVGGFDPVPQPDLPHSKPEPVQ"
     gene            complement(3392..3814)
                     /gene="rnpA"
                     /locus_tag="VEJ00_11190"
     CDS             complement(3392..3814)
                     /gene="rnpA"
                     /locus_tag="VEJ00_11190"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXX01773.1"
                     /translation="MPNAEKKDATASRGSDAPRLDFPRASRLVRRAEYNAVYRDGRRR
                     AGREFTVFLRPNGLAHSRFGWSIKKALGSAVRRNRIRRRLREIVRLNRQEIAPGWDIV
                     IHPRSSVATAGFLPLTDELLGLLPRASFLPPQPEKALP"
     gene            complement(3768..>3875)
                     /gene="rpmH"
                     /locus_tag="VEJ00_11195"
     CDS             complement(3768..>3875)
                     /gene="rpmH"
                     /locus_tag="VEJ00_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXX01774.1"
                     /translation="SRHKKHGFRARMKSAGGRKVLAQRRKKGRHSLTRV"
BASE COUNT          757 a         1195 c         1176 g          747 t
ORIGIN      
        1 gatctcggcg agaaccgctt ttctgatggt cgtcgccgga gtcctcagcc tttcacctgg
       61 gaggtcgtct tcgataattc cggcaatgtt ctggaccagg gttggctcgc caacggcctg
      121 ttacaacagc gcatgaggca cttagtcccg cagcagcccg tgcccgcacc gccgtcgagg
      181 ccaaaatctt gagagtcctc attttcagcg acattcacgg cgatcttcgc gcactcgacc
      241 gcatcctcca gcaacccgcc gatctgtaca tcgcggcggg tgacctggct accttcggta
      301 aaggactcga ccgctgcggc gaggttctcc gcccgctcgg ctcgcggctt tgggtgctgc
      361 cgggaaatca cgaaacgcac gaagacaccc gcgccttgtg tgcgcgcttc ggcttcatcg
      421 actttcaccg acagatgcgc agtgtcgaag gtggcacggg ctgcgcccag tgggcgggac
      481 tcggctatag caacatcacc ccgttccata cgcccggcga gtattcggaa gaggaaattg
      541 cgaacgcgct ggctgctttc gacaaggtcc cgggactgta tgtcgtcgcg cattgtccgc
      601 cgcgggacac cacactcgac gaatttgccc ccggccagca cggcggtagc tcttctttgc
      661 gcgcctggat caaacgggcg cagcctagct acctgttctg cggacacatc cacgaagcgg
      721 ccggcctccg cgagcgcgtc ggtgtgaccg agtgcatcaa cgtcggcaag caaggctata
      781 cggttgaaat ctaacgccga tggtttttca ggctggcgtc ttcacctcta ccaccggtgg
      841 gataaatgat cagctgccgt cggtccccca aaccttcgct caccgtgcga acgccttgcg
      901 ctccattgag ctccaaatga atgatgcgcc gctcgcgcga tgacatcggg ttgaagcggt
      961 gaggctgtcc ggtctcgcgg acgcgctgcg ccgccacgcg cgccgacagt ttcaattccg
     1021 ccagacgcgt cgcgcgatag tcgccgcagt ctaagcgaac gcggtcgtgc agccgcgggt
     1081 caagacgcag ccagcggtgg gcgatgtgtt cgagcgcgag cagcagctcc gcattccgct
     1141 ccagcagcag ctcgtcgtcg gttccccgaa atgtgaccgc gatatcggcg tcttccgagc
     1201 ccgctgcccc gggcgtaggc gccgagagat cccaccctac ggccagatgc atttcggcaa
     1261 tggcgagatc gagaaaccgc ttcaactccg ccaaggtgcg ctcacgatcc agaagtggct
     1321 cggccgagcc gcgccccccg cgtccctggc tggaagttct tttcctcgtg gcccctcgtt
     1381 cgtcgctcat ggttttttct ttttgaactt gctacgcgtg ggcagcggct gcgtccggtt
     1441 cagatacaac tgctggacca cgctgacaag attgctcgtg aaatagtaca ggttcagacc
     1501 gctcgataac tggaagaaaa tgaatgccag catgagcggc atcaaggcca tcatccgctg
     1561 ttgggcggga tcgacggtgg aagccgtcgg catcattttc gtcgagagat aggtggtcac
     1621 ggccatggcg atcggcaaga catagtaggg gtccttcgcc gacaaatcgt ggatccagag
     1681 aatccacggg gcgtgccgca attcgatcgc gccggccagc atcctgtaaa aagcccaaag
     1741 gaacggtatt tgcaagagca tcggcaggca tccgcccact tgcgaccatg gactgagtcc
     1801 gtgcttggcg tacatcgcca tgatttcttc attcatcttg ctgcggcgcg gatcggtcat
     1861 cgaatacttc cggtagcgat cctgaatcgc cttcatttcc ggcgcgatca cctgcatttt
     1921 tcgcatggag tggaaggtcc agatgcgaat cggaagcagc gccatggtca ggcccagcgt
     1981 caaaaccacg atggcccagc cgtaattggg gatgagacgg tggagccatt gcagaataaa
     2041 cagcatgggc ttggcgatga cacccgtcca tccgaattgc accagcccct ccagcgacgg
     2101 ctggaccttg ctgagaagcg ccagatcctt cgggcccacg tacgcgcgca acttcactgg
     2161 caccggagct gccgaaccta ccgccatctc cgccatcggc tcggccgtct gctgaccgtt
     2221 cgcgacgaga tgttgccact gcgtccaatg cgaaagatat aggacggttc cgttgggaat
     2281 aaacgcggcc gtaaaaaatt ggtcttcgat cccggcgaat tccatcgcgc cgggctgctg
     2341 gaaaggctgc gttgaattgc cagaaacgcc gagctttttg tattgaatcg tgctgaggct
     2401 cccgccttgc tgatagaaaa cgctgacgag ctgcgaggct cggtacacgg tgcggtcgcc
     2461 gaaacccccg cgccaggcca ctcccgaagc cagcggcttg ccatccagcg cggccgaggc
     2521 ttccagagac atttgatagt ccgaaccgaa gctcagcgtt ttgctgacgt ccaggtgccc
     2581 gttgctccag tgaaaactga cacggaccgg cgcattgaac gcattggcgt tcgccggaac
     2641 catcgaagtc ttgccggctt cgctgcttgc agctgcggtc tggacttcgt agaggcccgc
     2701 gttcgcatcc gcttccaact cgctatcgga aagttggagg gaaaagggcc agcccagctc
     2761 cttggcggca ttctcattga ccagatcgag cgggcgcgga ggcttttgat catcgaagta
     2821 cttcttgagc gtccagcttc gcaccacagc gccgcggttg gaaagctcga cgtgatagag
     2881 cgggctttca attgtgatga tccgctccgc ctgcgcctcc gcccgaggcg tgctgacccg
     2941 ttcgggcgtg gcactcaacg cggcttccac tcccggctgt gcctggccgg cgccgggcac
     3001 agtttgcgaa gcggtctggg ccggcttctc gggcgacgta acgggaggtt tgtagaagtg
     3061 caaccaaaca aaggtgatga gaacgaccag cactacgaag agaatcgcgc ggacctggtc
     3121 cgacatctcg ctcactggac cggctccggt ttgctgtgcg gcagatcggg ttggggaacc
     3181 ggatcgaatc cgccaaccgt aaatggccga cagcgcagca gcctcttcat ggccagcacg
     3241 aagccgcgcc aggcgccgtg aagctggatg gcttcatacg cgtagttcga gcaggatggc
     3301 tggaacttgc aagctccgcc gaaaaacggt gaaaaaaaaa cgatgtagca tcgcagcaag
     3361 aacaacaagg tgcacgcgcc caacggcctc gctacgggag ggccttctcg ggttggggtg
     3421 ggaggaaaga tgcccgcgga agaaggccga ggagctcgtc ggtcagcggc agaaaacctg
     3481 ccgtggctac tgagctgcgc ggatgaatga cgatatccca tcccggggca atctcctgtc
     3541 ggttaagcct gacgatctca cgaagccggc gccggatacg attgcgccgg actgcgctgc
     3601 ccagcgcctt cttgatgctc cagccgaaac ggctgtgagc gaggccattg gggcgaagga
     3661 aaacggtgaa ctcgcggcct gctcgcctcc ggccgtcccg gtacaccgcg ttgtattcgg
     3721 cgcggcgcac cagacggctg gcacgaggaa aatcgagccg cggcgcatca gacccgcgtg
     3781 aggctgtggc gtcctttttt tcggcgttgg gcaagaacct tccgtccgcc cgcgctcttc
     3841 atgcgtgcgc ggaagccgtg tttcttgtga cggct
//
LOCUS       DASPUL010000469         3628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_496421, whole genome shotgun sequence.
ACCESSION   DASPUL010000469 DASPUL010000000
VERSION     DASPUL010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496421"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VEJ00_11200"
     CDS             <1..629
                     /locus_tag="VEJ00_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01775.1"
                     /translation="YFVYTNYATEIRDTTEHVVCEAMIEPVAFEAVDEDLCRAVIDGR
                     ITPKRFDSLPEGTWQVEGWTLDQDRRPGTALIDIDNDGKLDNIASLKAEDGRRLELAS
                     IGISPPALPADPTNELLGQLQYELPGQGPICQVKPFASGCRAFDFWRFDPFQYNDRTY
                     IDKYHANDYGKSDRSIVELANGKVRRMCRLGYRIPGWRAAAVQRTPEK"
     gene            complement(872..1462)
                     /locus_tag="VEJ00_11205"
     CDS             complement(872..1462)
                     /locus_tag="VEJ00_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01776.1"
                     /translation="MKAFFRTLAVLLLTVLVGPCIAYADEWPYAKVTCDKETSQLIVE
                     ELETDERERIPREPGVQDLMALTQLRTLDGPAGTQDYRVPRSDFRFECDLGRSHFKVK
                     ISPWKFNQKVAGMCGGYDPSIQLSVWRASRKLLDKLIFTGFCNPPESDFGIVAVKLLE
                     GEQAAVILIGGEGGRVERRIPFSKLGKLHREQLHPD"
     gene            complement(1570..2889)
                     /locus_tag="VEJ00_11210"
     CDS             complement(1570..2889)
                     /locus_tag="VEJ00_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXX01777.1"
                     /translation="MKILLQCLWLLAMLANFGSSQADDQSPRKSISEYFKNNKLISSR
                     HAFYNAAKTQRVEYYCEDENRPGGANEGASNPANVACAAALFLKHSGQWVFSDAIDLR
                     YGSVEKFSGHRLEVEMLEYGAEDALCCPSERWTRIFDTKTGKLIEDIASKRQPLIAEA
                     VKDGLDQRRIGELISQGNDINAKDRYGQTALMAAAEKSNVEITRLLLAHHADVNATRN
                     NGKTALVIAAGQGSVEIVKLLLDKGADANIDSDEGRSALYYAIRSGNGDIARLLVAGG
                     ADVNAETQRGETVLMEAAVSGREDIVRLLLEKGANIKTTYEGTSILTAAAERSRCKIV
                     RLLLDNGANANEENWEVLMRAAAYSRDTDCLQALLEKGANINVKDRAGQTPLFYAAQY
                     GPVANVEWLLKHGADPNVIAYGQTPLEAARKLGNKDVVRFLEKQTPR"
BASE COUNT          763 a         1026 c         1042 g          797 t
ORIGIN      
        1 tgtatttcgt gtataccaac tacgcgacgg aaattcgaga cacgaccgag cacgtcgtat
       61 gcgaggcgat gattgaaccg gtggcgttcg aggcggtcga tgaagatctc tgtcgcgcgg
      121 tcattgatgg gaggatcacg ccgaaacgct ttgattcgct cccagaaggc acctggcaag
      181 tggaaggatg gacactggac caggaccgac gtccaggtac cgcgctcatc gacatcgaca
      241 atgacggcaa actcgacaac attgcgtcac tcaaggcgga ggacgggagg cggcttgaat
      301 tggcctcgat tggcatttcc ccgcctgcat tacctgccga cccgaccaat gaactgctcg
      361 gccagctgca gtacgaactg ccgggccagg gaccgatttg ccaggtaaag ccctttgcca
      421 gcgggtgtcg cgcgttcgac ttttggaggt ttgacccatt tcaatacaac gatcgtacct
      481 acatcgataa ataccacgcg aatgactatg gcaaatccga tcgcagcatt gttgagctcg
      541 cgaacggaaa agtcaggagg atgtgtcgtt tgggctatcg gattccggga tggcgtgcag
      601 cggccgtgca gcggacgcca gagaagtagc ctgctgagaa gggtgacgat tagagcactc
      661 tggcgcccgc aactcgtgtc gagatggcag aactgtagcc gttgcacgct gctcccgacc
      721 cacagccgtc ctagcgaccg ctgcaaagcg gccacctaat cgaggggaag gctgacactc
      781 gacaggttgg acatctactg ggtcgaaatt cgacccggcc ggtcggtccg cccttcgctt
      841 cgaggccaaa acaaggcgga ctgggtgaag atcagtcggg gtgaagctgc tccctgtgga
      901 gcttcccgag tttggagaaa ggtatgcgcc gttctacgcg ccctccctca ccacctatta
      961 ggatcactgc tgcctgttca ccttctaaga gcttaactgc gacgatccca aagtcgctct
     1021 cgggaggatt gcagaagccg gtgaagatca atttgtcgag cagctttcgt gacgcccgcc
     1081 agacgctcag ctggatgctt ggatcatagc caccacacat gccagcaacc ttctgattga
     1141 acttccaggg agagatcttc actttgaagt gcgagcgacc gagatcgcat tcaaacctga
     1201 agtctgacct tggcacccga taatcctgtg ttcccgcggg cccatccagg gtacggagct
     1261 gggtaagtgc cattagatcc tgcaccccgg gttcccttgg aatccgctcc cgctcgtcag
     1321 tttcgagctc ctcgacgatc agttggctgg tctccttgtc gcacgtgact ttcgcgtatg
     1381 gccactcatc ggcgtacgca atacaaggcc cgaccagcac cgtaagtagg aggacagcaa
     1441 gcgtcctaaa aaatgccttc atttgtactc gacagatcgc ggcccgcttt gtcgaccttc
     1501 ccaatgcaga tccccggcat tccttatgct gtattgcgtt cgagtaacga gacctgatac
     1561 ttagcaggat tatcggggcg tttgcttctc gagaaaccgc accacgtcct tgttcccgag
     1621 ttttctagcc gcctccagcg gcgtttgacc ataggcaata acatttggat cggcgccatg
     1681 cttcagaagc cattcgacat ttgcgaccgg tccatactgg gctgcgtaaa acagtggtgt
     1741 ctgaccagcg cggtctttca cattgatgtt tgcgcccttc tcgagtagag cctgaaggca
     1801 atcggtgtct ctgctatatg ccgcggctcg catcaaaacc tcccagtttt cctcgttagc
     1861 attcgccccg ttgtcaagta gcaatcggac gatcttgcac cttgatcgct ctgcagcggc
     1921 cgtcaatatc gatgtgccct catacgtcgt cttgatgttg gcgccctttt caagcaacag
     1981 ccgcacgata tcctctctgc cagagacagc agcttccatc aagacagtct cgccacgctg
     2041 cgtttcagca ttgacatctg ctccgcctgc tacaagtagt cgggcaatgt caccgttgcc
     2101 gctccgaatc gcgtagtaaa gtgcagagcg gccttcgtcg ctgtcgatgt tggcgtccgc
     2161 gcctttgtcc agcaagagct tcacgatttc aaccgagcct tggcctgcag cgatgaccaa
     2221 cgcagttttt ccgttgtttc ttgtcgcgtt gacgtccgca tgatgggcta gaagcaatct
     2281 tgttatctct acgttgcttt tttcggctgc agccatcaac gccgtttgcc cgtatctatc
     2341 cttcgcgttg atatcgttgc cctggcttat cagttcgccg atgcgccttt gatcaaggcc
     2401 atctttgacc gcttcagcga tgagcggctg tcttttcgaa gcgatgtcct caatgagctt
     2461 tccggttttg gtatcgaata ttcgcgtcca gcgctcactc gggcagcaca aagcatcctc
     2521 ggcaccatat tccaacatct cgacctccag cctgtgcccg ctgaatttct ctaccgagcc
     2581 gtaacgcagg tcgattgcgt cactgaatac ccattgcccg gagtgtttga ggaacaaggc
     2641 cgccgcgcaa gctacatttg cgggattgct tgcgccctcg ttggccccgc cgggtcgatt
     2701 ctcatcttca cagtagtatt cgacccgctg ggtcttagcg gcattgtaga aggcatgccg
     2761 ggagctgatc agtttgttgt tcttgaagta ttcggaaatc gacttccgag gagattggtc
     2821 gtcggcctgg ctcgagccaa agttggcgag catggcaaga agccataagc attgaagaag
     2881 tattttcacg gtagctgctc tattcagtac gcctatccag agaggcgagg gtgcgagctt
     2941 tcggactatg gttcttactc ctagcgtccg gggctgttgc gacagatccc tcccgacgga
     3001 gattcgcccg tcagtaggat catgctcatt tacgcgcgag ggagggcaga cttgaggtgg
     3061 gcagccaggt cgggggctgc ccagaagcga caagtgtcgg gttctgcgga aatttggggt
     3121 tgcgccccca aggactgcag ttggcgtgcg ccgtgataag gcggcgcttt ccagtgggtg
     3181 caagccccac ccggcaaccg ctccagccgg taagcaaccg gagcagctat ggaggaaacg
     3241 aaatggctga agccttcggg taaagcgtgt cacaaaatac ggtgacagcg cgagtgtgca
     3301 ggccgtaacg cgactgaacg ccgagcaagc ctcgaaaagg tcgatgcgca ggccgacccg
     3361 actgccatat cggggaaggc tgacacggct ggggagaatg agcgaagaac agccccagtc
     3421 gctgtgccgg gctagtggcg acggcgtgta cacaaggaaa gcgcgcgcaa cacgggaagc
     3481 ccgatagcgt ggtcggtcgt gacgaccaac acgagaccgg tgacggtcag acagggcgct
     3541 ttcgggtggc ggagaggccc gcagtaccga ggaggccggg taacgccgga ggagggaagg
     3601 ggcctcagtt caaggtgggc gctgaaag
//
LOCUS       DASPUL010000470         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1732947, whole genome shotgun sequence.
ACCESSION   DASPUL010000470 DASPUL010000000
VERSION     DASPUL010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1732947"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VEJ00_11215"
     CDS             <1..910
                     /locus_tag="VEJ00_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HXX01778.1"
                     /translation="ESVIARAKSLLKPEAHEAADLIAYLHRSRDELDRMQRQMAAERR
                     ALEAERAKLRTEWVARQQQRIKELEGQFAEIHKRFEENVTRVVAAVQERELRASLEKS
                     TRRKLQETRSEAREELNAAVVQTISESQADLGVRAEAAESVSAEQLQAGRRVRVRGFR
                     KPVIFRRLDGSAAEIEAGPLRMKVALDEITGIETAGEPSKAPITGTIRSSTTVSTQPG
                     AGSVSDEINVIGLTVEQATELVDKFLDEAALAHKPRVRIIHGHGTGALRRGLGEFLKG
                     HALVERTATEHPDRGGAAITVVELRA"
     gene            1062..1661
                     /locus_tag="VEJ00_11220"
     CDS             1062..1661
                     /locus_tag="VEJ00_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01779.1"
                     /translation="MPGTSFVAAMMLAGGLVHALRMLPRGISFSFIRLVTSIFIGLVA
                     YPAGFLSVFLEVWVLFAWSQGDAHGVEHGALDLIIGYLTMGGMFFVGAAVTLLILTAA
                     FVFVARYWPRRAFRALASLIGVVSLGSMLASFLHFAVYLHRTPDKIALLNFLGEPLLV
                     FFGFAKGIPLAVLVGEPLFGAIIGHWLYEAAVEWSAESA"
     gene            1677..3446
                     /gene="dnaG"
                     /locus_tag="VEJ00_11225"
     CDS             1677..3446
                     /gene="dnaG"
                     /locus_tag="VEJ00_11225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HXX01780.1"
                     /translation="MAEAGSFADRVKQQADIVRVVGEYVRLKKTGKDFSGLCPFHQEK
                     TPSFTVSPIKQIFYCFGCGKGGDVFNFVMEVEKAPFPEAVRTVAEKCGIAIPGPKERS
                     PEERKETQQRSALLEMHREAQAFFVKQISGTLEGKAARAYLEDRGIDNKTIERFGIGY
                     APSNGDALLRFFKQKRYDEKLLAESGLISRGEQGGRLFDRFRRRITFPIANDSGKIVA
                     FGCRALGDDQPKYLNSPETPIYSKSNVLYHLDRAKEALRRSDFAVLVEGYMDAIAVAR
                     AGISNVVASCGTSLAEPQMKLLSRFTKRVILNYDPDAAGQAATERSVALLLEQDFEVR
                     VLALPTIGTKKADPDLFIREKGVDEYTRLLKDAPPYVDYLIGRARQMDLSTGEGKRRA
                     VNFLLPYVQKIPNAILRSEWATRIAQQLHLDEPVLRAALSKAAKERRSEVKVQPELVS
                     RPAKPVERRLIRMLAEASGFRRELSQQLHGALLYQGLETERIFSALILASMADEPVQP
                     TEVAERLEERDRRLFYEILFEEASEATWEEAQSCVETLQQKLAERELAEVQRSIEANP
                     PAPELRGLLAKKQDLMRRLAAAR"
BASE COUNT          693 a         1014 c         1103 g          703 t
ORIGIN      
        1 cgagagcgtc atcgcccggg cgaagtccct gctgaagccg gaggctcatg aggccgctga
       61 cttgatcgct tatctccacc gtagccgcga tgagctcgac cgcatgcagc gccaaatggc
      121 ggcggagcgc cgcgctctgg aagcggaacg cgcaaagcta cggaccgaat gggtcgcccg
      181 gcaacagcaa cgtattaaag agctcgaagg gcagtttgca gagatacaca agcgtttcga
      241 agaaaacgtc acccgtgtgg tggcagccgt acaggaacgc gaattgcgcg cttcgcttga
      301 aaaatccacg cgccgcaagc tgcaggaaac gcgcagcgaa gcacgcgaag agttgaatgc
      361 cgcggtggtg cagacgattt cagagtcgca agccgacctg ggcgtccgcg cagaggcggc
      421 cgaatcggtc agcgcggagc aactccaagc gggtagacgc gttcgcgtac ggggcttccg
      481 caagcctgtc attttccggc ggcttgacgg ctccgctgcg gagatcgagg cggggccttt
      541 gcgcatgaag gttgcgctgg acgaaatcac cggtatcgaa acggcggggg agccgtctaa
      601 agcaccaata acgggaacga ttcgctcgtc cacgacggtg agcacgcagc cgggggcggg
      661 ttccgtcagt gatgagatca acgtcatcgg cttgaccgta gagcaggcca ccgagttggt
      721 ggacaagttt ctcgatgagg ctgcgctggc gcacaagccc cgcgtgcgca ttattcacgg
      781 tcacggcact ggtgcactgc ggcgaggctt gggagagttc ctgaagggac atgcgcttgt
      841 cgaacgcacc gccacggaac accccgaccg gggaggcgct gccatcaccg tcgtcgaact
      901 ccgcgcctga tgtcctcggc cgttcccatg tgagggtcgc ggcaattttc tttctcttcg
      961 gtctggctgc gcttttcccg actgtaatcc tggaaagggc agcaaacggc gggcccggcg
     1021 cgtacatttc gcgatttccc tttttggtcc gaaccctggc tgtgcctggc acgtcattcg
     1081 tggccgccat gatgctcgct ggtggcttgg ttcacgccct gagaatgctt ccccgcggaa
     1141 tttccttttc gtttatccgg ctggtgacta gcattttcat agggctggtc gcctatcccg
     1201 ccgggtttct ctccgtgttt ctggaggtct gggtgctttt cgcgtggtcc caaggcgacg
     1261 ctcacggcgt ggagcacggc gcattggacc tcatcatcgg ttaccttacg atgggaggca
     1321 tgttcttcgt tggcgcggca gtgacgctac tcatccttac tgcggctttc gtcttcgtcg
     1381 ctcgctactg gccgcggcgc gcatttcgcg ccttggcctc gctcatcggc gtggttagcc
     1441 ttggcagcat gctggccagt ttccttcact tcgccgttta tttgcaccga acacccgaca
     1501 agatcgcttt gttgaacttc ttgggcgaac cgctgctcgt tttcttcggc ttcgccaagg
     1561 gtattcccct agccgttctg gttggcgagc ctctcttcgg cgccatcatc ggccactggc
     1621 tttacgaagc cgctgtggag tggtctgccg agtccgcgta gaatagcttc agggaaatgg
     1681 cggaagcagg ttcttttgcc gatagggtga agcagcaggc ggacattgtt cgcgtggtgg
     1741 gcgaatatgt gcggctgaag aaaacgggca aggacttttc ggggctttgt ccgtttcacc
     1801 aggaaaaaac gccctccttc accgtctctc ccatcaaaca gatcttttat tgcttcggct
     1861 gcggcaaggg cggcgatgtc ttcaacttcg tgatggaagt ggagaaggcg ccgtttccgg
     1921 aagcggtgcg taccgtcgcg gaaaaatgcg gcatcgcgat tccggggcct aaagagcgct
     1981 cgccggaaga gcgcaaggaa acccagcagc ggtcggcgct gctggagatg catcgcgaag
     2041 cgcaagcgtt tttcgtgaaa caaatctcgg gaacgctgga aggcaaagct gcgcgcgcct
     2101 atctcgaaga tcgcggcatc gacaataaaa cgatcgagcg cttcggcatc ggctacgcgc
     2161 caagcaatgg cgacgcgttg ttgcgcttct tcaaacaaaa acgatacgac gaaaagctgc
     2221 ttgccgagtc cggactcatt tcgcgcggcg agcaaggcgg acggcttttt gatcgcttcc
     2281 gccggcgcat tacctttccc atcgccaatg actcgggaaa aatcgttgcc tttggctgcc
     2341 gcgcgctcgg cgacgaccag cctaagtact tgaattcgcc ggaaacgccc atctactcga
     2401 agagcaacgt cctctatcac ctcgaccgcg cgaaggaagc gctgcggcgc tcggatttcg
     2461 ccgtgctggt tgaaggctat atggatgcga tcgcggtggc acgtgcggga atcagcaacg
     2521 tggtggccag ctgcggcacg agcctggcgg agccgcagat gaagctgctg agccgcttca
     2581 ccaagcgggt tatcctaaat tacgaccctg acgccgccgg ccaggcggct acggagcgct
     2641 cggtcgcgct gctgctcgag caggatttcg aggtgcgcgt gctggcgctg cccacgatcg
     2701 gcaccaaaaa agcggacccc gatctgttca tccgcgaaaa gggcgtggac gaatacaccc
     2761 ggcttttgaa agacgcgcct ccctatgtgg actatttgat cgggcgagca cgccaaatgg
     2821 atttgagcac tggcgaaggc aaacggcgcg ctgtgaattt tctcttgcca tacgttcaaa
     2881 agatcccgaa tgccattctg cgttcagaat gggccacccg cattgcacag cagcttcatc
     2941 tcgacgagcc cgtccttcgc gccgcactca gcaaggccgc gaaggaacgg cgcagtgagg
     3001 tgaaagtaca gccggagttg gtgtcgcgac cggcaaaacc ggtggagcgc cggctcatcc
     3061 gtatgcttgc cgaagccagc ggctttcgcc gcgagttgtc gcagcagttg catggcgcgc
     3121 tgctctatca ggggctcgaa acggagagga tcttttccgc gcttattcta gccagcatgg
     3181 cggatgaacc ggtgcagcct acggaagtcg ccgagcgtct cgaggagcgc gaccggcggc
     3241 tcttctatga aattctcttc gaagaggcca gcgaggctac ttgggaagag gcgcagagct
     3301 gcgtggagac tctgcagcag aagctggccg agagggagct ggccgaggtg cagcggagta
     3361 tcgaggccaa tccaccagca cccgaactgc gggggctgct cgccaagaag caagatctga
     3421 tgaggcggct cgcagcagca aggtagaacg ggccgagcgc gagaatttcg gatgcatcga
     3481 agcacttttc catagcattc gcttgcgaac gag
//
LOCUS       DASPUL010000471         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1183472, whole genome shotgun sequence.
ACCESSION   DASPUL010000471 DASPUL010000000
VERSION     DASPUL010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183472"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VEJ00_11230"
     CDS             complement(<1..519)
                     /locus_tag="VEJ00_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXX01781.1"
                     /translation="MLAATKVKIAPGRLLIDGQWVEASRSFDTINPATGEVLTQVAEA
                     SSADVDHAVAAARRAFEDRNGAWRKLSASERGRLLWKLADLLEKNIDEFAELETLDNG
                     KPIFESRYVDMPMVIDVLRYYAGLATKIHGETVNTFEAAFTYTLREPVGVVGLIVPWN
                     FPLLLASWKLGPA"
     gene            complement(593..1414)
                     /locus_tag="VEJ00_11235"
     CDS             complement(593..1414)
                     /locus_tag="VEJ00_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXX01782.1"
                     /translation="MATTLQPATAETTKQLINYRTDGGVAVIEMCDPPANTYTYEMNR
                     QLDDAILKARMDNDVYVIVLTGAGDKFFSAGANIKMLSSVDPTFKYYFCLHANETLLR
                     LEHTPKLVIAAINGHCVGGGLEIAMAADIRIARKDAGKIGLPEVNLGVLPGTGGTQRL
                     SRMVGKSKAIELMVTGNTYSFEEALELGIVNDIFEREGFMDNIMEYARQFCPPNKAAK
                     AVGRIKRSVQTGWEIPLESALAVERENQQLLFQSEDAKEGLAAYVEKRPATFKAK"
     gene            complement(1572..2219)
                     /locus_tag="VEJ00_11240"
     CDS             complement(1572..2219)
                     /locus_tag="VEJ00_11240"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXX01783.1"
                     /translation="MATPLTAARVGSKPRAAAPASRYDRRLAEILTHATEVFCKKGYE
                     GASMRDLSRASGMSLAGLYYYFESKERLLFLIQKHTFSTIVERLKARLEGVNDPEERI
                     RIFILNHLEYFLANQAAMKVLSHEAEALKNGFATEVAAIKREYYRICVGLLDDLKRER
                     GLQFSTRIAVLSLFGMMNWIYTWHNPRVDADAESIAGEMSEIFLSGVMTEAKSRK"
BASE COUNT          430 a          677 c          683 g          512 t
ORIGIN      
        1 cgccgggccc agtttccagg aagcaagcag caaggggaag ttccagggaa cgatcagccc
       61 aaccacgccg actggctcac gcagcgtgta ggtgaaggcc gcttcgaaag tattgacggt
      121 ctcgccgtgg attttcgtcg caagtcctgc gtagtagcgc aggacgtcga tcaccatcgg
      181 catatcgacg tagcgtgatt cgaaaatggg cttgccgttg tcgagggttt cgagttcggc
      241 gaactcatcg atgttctttt cgagcaggtc ggcgagtttc caaaggaggc gtccccgctc
      301 gctggccgag agcttgcgcc aggcgccgtt gcggtcttcg aaggcgcggc gggcagcagc
      361 gacggcgtga tcgacatcgg ctgaggatgc ttccgcaacc tgtgtcagca cttcacccgt
      421 ggcggggtta atggtatcga atgacctcga cgcctcgacc cactgcccgt cgattagaag
      481 acgacctggc gcaattttca cttttgttgc cgcaagcatt ttggtccttg cctcaagccg
      541 agctgcgtta ggcggacggg cgaggcgccc gtccccacaa gcgcttccct gcctatttcg
      601 ccttgaacgt cgccggccgc ttctccacgt acgccgccag cccttctttc gcgtcttcac
      661 tctggaacag caactgctga ttctcgcgtt cgacggccag cgccgactcc agcggaattt
      721 cccagccggt ctgcaccgag cgcttgatac gtccgacggc ctttgccgcc ttgttgggcg
      781 ggcagaactg gcgggcgtat tccatgatgt tgtccatgaa gccttcgcgc tcgaagatgt
      841 cgttgacaat accgagctcc agcgcctctt cgaacgaata cgtgttgccg gtaaccatca
      901 gctcaatcgc tttgctcttg cccaccatgc gcgagaggcg ctgcgtaccg cccgtgccgg
      961 gcagaacgcc aagattcact tccggcagtc cgatcttgcc tgcgtctttg cgagcgatcc
     1021 gaatgtcggc ggccatggcg atttccagtc ctccgccgac gcagtggccg ttgatggccg
     1081 caatcaccaa cttgggcgtg tgctccaggc gcaggagcgt ctcgttggcg tgcaggcaaa
     1141 agtagtactt gaacgtcggg tcgacgctcg aaagcatctt gatgttcgcg ccggcagaga
     1201 agaacttgtc gcccgcgccg gtcagaacga tcacatagac gtcgttatcc atacgcgcct
     1261 tcaggatggc gtcgtcgagc tggcggttca tctcgtaggt gtaagtgttg gcgggcggat
     1321 cgcacatctc gatcacggcg acgccgccgt cggtgcggta gttgatgagt tgcttggtgg
     1381 tttcagcggt tgccggctgc agtgtggtcg ccatgtcaga tgactccttg atcgttattg
     1441 tcagtggccc tcaggggctg aagccccaat tctatttgcg tctagcggca cggctgaagc
     1501 tgtgcccttc ccaaaacccc ttgcgcttcc caaatttgtg ccctctcaaa gtacgcgcta
     1561 acacctcagc gctacttgcg gctcttcgct tctgtcatga ccccgcttag aaaaatctcg
     1621 ctcatctcgc cggcgatcga ctccgcgtcg gcgtcaaccc gcgggttgtg ccaggtgtag
     1681 atccagttca tcatgccgaa caagcttaga actgcaatcc ttgtggaaaa ctgcaggccg
     1741 cgttcgcgct tgaggtcgtc gagcaaaccg acgcagatgc gatagtactc ccgcttgatg
     1801 gcagccacct cggtggcgaa accgttcttg agggcctcgg cttcgtgcga cagcactttc
     1861 atggcggcct ggttggccag gaaatattcc aggtgattga gaataaaaat ccggatgcgc
     1921 tcttccgggt cgttcacgcc ttccaatcgc gccttgagcc gctccacgat cgtcgagaag
     1981 gtgtgcttct ggatgaggaa gagcaggcgc tccttggatt caaagtagta gtacaagccg
     2041 gcgagtgaca tgccgctggc gcgcgagaga tcgcgcatgg acgcgccctc gtatcccttt
     2101 ttgcagaaga cttcggtggc atgagtcaga atctccgcca aacgacggtc atagcggctg
     2161 gctggggccg ctgcgcgggg cttggaacca actcgggctg cggttaaagg ggtggccata
     2221 ctgccatcat tcgaacgttc gttcgaaatc agtatagcgc gatgtgattg caatcacaag
     2281 tgacgtgtaa ctgatggaat gt
//
LOCUS       DASPUL010000472         3029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_230013, whole genome shotgun sequence.
ACCESSION   DASPUL010000472 DASPUL010000000
VERSION     DASPUL010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230013"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..2742
                     /locus_tag="VEJ00_11245"
     CDS             160..2742
                     /locus_tag="VEJ00_11245"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXX01784.1"
                     /translation="MLALPAVVAMLCAGQSSYTLESGAPHKLAVVVTDENGVAVARAR
                     VQLRPPQPGAPLRCETDFAGRCKFEALSAGAYDLTVDKTGFYASSVPGVQVGATANVD
                     VTLSHQQAVRETVEVVASGPTIDPSQVASKDELTGTEMVDVPYPGPHDYHGALNFIPG
                     VTPDGFGQLHVAGAEGYQTLVLLDGFNVSQPTNGQLAVRTSIESFRSMEVTPSREPAE
                     LGKGSGGVIGLNTAMGRDQYRFTSTDFVPAVWTTQGRLQAQWTPIDTFSGPIIKGKAW
                     FIDALDGEFDDNVDTSLPVGKDRDYIWRADNLAKVQANLTNRNILTASFLSNYYHDKY
                     SGLGFLVPQSATPTEIETAYFGSLKDQYYFHNGTLLETGFGVDQYDTTLRPQGDLPYI
                     ELFFSAAGNYYLHEDALARRAQGMTNLYLPPHEWLGRHDVKVGFDVDRLSYNAQFLRQ
                     PISFEQQGAPGPGQTVLPCNTDANGVPIAPYTCTRYSTFSGGAFAPSYNAEASVYVED
                     RWLIGNRLLIEPGLRLDWDELVRTPLLSPRLAGTFRLDAEGTTKFSAGIGIIYDATNL
                     GLIHQPLEGQRTDYFFKCVPMPQSACGTTEPTDPSGNPTAGPVPVPTTFEVDRSALDA
                     PRYVNSSISLEKKLPFDTFLKLECMDKRGTHAFAYNTLNGAVDGVYLLGNQRDDRYDA
                     FTVSLRHRFHGHYEVFGAYTRSKSRSNQIFDFSLDIPLLSPQLPGPYPWDTPNRFVGW
                     GVLPGPSLPVIHKFDIVYSAEARTGLPFYGTTDQGEIAAGFPPGNSLRLPTYYTLNLQ
                     AEKRVHLFGRYWAGRIGFNNITNHPNVGVISNIDSTHPAPTFFDSNGGRAFALRIMLL
                     GKDK"
     gene            complement(2780..>3029)
                     /locus_tag="VEJ00_11250"
     CDS             complement(2780..>3029)
                     /locus_tag="VEJ00_11250"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXX01785.1"
                     /translation="APQLDLRAMYAKSGMVQGLWLSYLSQRREIMEPAWRQLSAWIAE
                     GKLIPQIGHVFPMDRAVEAYKLLEEGRNYGKVVLKIST"
BASE COUNT          625 a          961 c          840 g          603 t
ORIGIN      
        1 agaaagtgtc cagttcttca cctcgtgcga aacacaacgc ttccgacact tccttacatt
       61 gtccccacca tggctagtct aggattgagt gcttgaaaga ggcgaaggaa gagaggctca
      121 gatcattccg ctgtgaccgg ggttgcgaaa tccgcacttt tgctggcctt acccgccgtt
      181 gtggcgatgc tgtgcgccgg gcaatcgtcc tataccctgg aatcgggcgc gccgcacaag
      241 ttggctgtcg tcgtgacgga cgaaaacggg gtcgcggtgg cccgcgcgcg agtgcagttg
      301 cggcctccgc agcctggcgc tccgctgcgc tgcgaaacgg atttcgcggg tcgctgtaaa
      361 ttcgaggccc tgtcagccgg agcttacgat ctcacagtcg acaagactgg cttctatgcg
      421 tcgtcggtgc caggcgtgca ggtcggcgca accgccaacg tcgacgtgac actctctcac
      481 cagcaagctg tgcgagagac ggtggaggtc gtcgcttctg gaccgacaat cgaccccagc
      541 caagtcgcgt ccaaagacga actcaccggc acggaaatgg tcgacgtccc ctatcccggt
      601 ccacacgatt accacggcgc gctgaacttc atccccggag tcacccccga tggtttcggt
      661 caactgcatg tggctggagc tgagggctat cagacgctgg ttctgctgga cggtttcaac
      721 gtgagccagc cgaccaacgg gcaactggcc gtgcgcacca gcatcgagtc gttccgctcg
      781 atggaagtca ctcccagccg cgaaccggct gaattaggca aaggatccgg cggcgtcata
      841 ggcttgaaca ctgccatggg cagagatcaa tatcgcttca cttcgaccga tttcgttcct
      901 gcggtctgga ccacccaggg ccggctccag gcgcaatgga cgcccatcga cactttctcc
      961 ggtccgatta tcaaagggaa agcatggttc atcgacgccc tcgacggcga atttgacgac
     1021 aacgtcgaca cctcactccc ggtgggcaaa gatcgcgact atatctggcg ggccgacaat
     1081 ctggccaagg tgcaggcgaa tctgacgaac cgaaatatcc tcaccgccag tttcctgtcg
     1141 aactactatc acgacaaata cagcgggctg ggatttctgg ttcctcaatc ggcgactccc
     1201 accgagattg aaaccgcata ttttggctcg ctgaaggatc agtactactt tcacaacggg
     1261 acgctgctgg agacgggctt cggcgtcgac caatacgaca caacgctgag gccgcagggc
     1321 gatcttccgt acatcgagct gttcttctct gccgccggca actactacct gcacgaagac
     1381 gcgctcgccc ggcgcgcgca gggaatgacg aacctgtatc tgccgccgca tgagtggctg
     1441 ggaaggcacg acgtcaaggt tggattcgac gtcgaccgtt tgagctacaa cgcgcagttt
     1501 ctgcggcagc caatctcatt cgagcagcaa ggcgcgccgg gaccgggaca aacagtcttg
     1561 ccatgcaaca ccgacgccaa cggtgttccg atcgcgccgt acacgtgcac gcgctattca
     1621 actttctccg gcggcgcttt cgctcccagc tataacgcgg aagccagcgt ctacgttgag
     1681 gatcgctggc tgatcggaaa tcgcctgctg atcgagccgg gcctgcgtct cgactgggat
     1741 gagttagtgc gcacgccgct gctgtcgccg cgcctggccg gaacctttcg cctcgacgcg
     1801 gaaggcacca ccaagttctc ggcagggatt ggcatcatct acgatgcaac caacctgggc
     1861 ctgattcatc aaccgctcga aggacaacga acggactact tcttcaaatg cgtgcccatg
     1921 ccccagagtg cctgcggcac aaccgagcca acggacccga gtggcaatcc caccgctggg
     1981 cccgtgcctg ttcccacgac atttgaagtc gatcgaagcg ctctcgatgc gcctcgttac
     2041 gtcaattcaa gtattagttt agagaagaaa ctccccttcg acactttcct gaaactcgaa
     2101 tgcatggaca agcgcggcac gcacgccttc gcgtacaaca cgttgaatgg cgcggttgat
     2161 ggagtttatc ttctaggaaa tcagcgcgac gaccgctacg acgcgtttac agtcagcttg
     2221 cggcaccgct ttcacggaca ctatgaagtt ttcggagcct acacacgctc caagtcccgc
     2281 agcaaccaga tcttcgattt cagcctcgac atcccgctgc tgagcccgca attgcccggc
     2341 ccgtacccgt gggacacgcc gaatcgtttc gttggctggg gcgtgttgcc ggggccaagc
     2401 ctgccggtca ttcacaagtt tgacatcgtc tactcggccg aggcgcgcac cggcttgcct
     2461 ttctacggaa ccaccgacca gggcgaaatc gccgccggct ttccaccggg caactcgttg
     2521 cgcttgccga cctactacac gctgaacctg caggcggaaa agcgggttca tctgttcgga
     2581 cgctactggg ccggtcgcat cgggttcaac aacatcacca accaccccaa cgtgggagtc
     2641 atctccaaca tcgattccac ccaccctgct ccgaccttct tcgatagcaa cggcggccgc
     2701 gccttcgcct tgcgcatcat gctgctgggg aaagacaagt gagtggtgtt ctcgcgacct
     2761 ggccctgtaa acggcgtgtt taggtggaaa tcttcagcac cacttttccg tagttccttc
     2821 cctcctcgag cagcttgtaa gcttcgacgg cgcggtccat cggaaacaca tgaccaatct
     2881 gcggaatcag cttgccttcg gcgatccatg ccgagagctg cctccacgcc ggctccatga
     2941 tctcgcgcct ttgcgacagg taagacagcc agagcccttg caccatgccg gatttggcat
     3001 acatggcgcg caggtcaagc tggggagca
//
LOCUS       DASPUL010000473         3662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_767163, whole genome shotgun sequence.
ACCESSION   DASPUL010000473 DASPUL010000000
VERSION     DASPUL010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767163"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="VEJ00_11255"
     CDS             <1..1276
                     /locus_tag="VEJ00_11255"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HXX01786.1"
                     /translation="NTEAMFPHFKDTRFWLSGQANFIFQTHPEFHAAYSGAHSFGPHY
                     EKATSRVLTMFGGIRFNNSTEFVGALEEAGGSALSLGFGMAGNTDLDIVRNPLLSKAP
                     YLGRGMFHKVFALSDDKVENQRNYLSLFDELPRRRLEIRFGKFSMPDFFDINSVGSDT
                     HFQFSNWTIDNNGAWDYAADTRGYTVGFTADFEDRNWGFRFGEALMPRVANGIDLVWK
                     PWQAHAESFEYELRHGFLPKKSGVVRLLAFTNYANMGIYRQQLALAEQQGTTPDITNH
                     PWHITRKYGFGVNLEQNLTHYLTAFARFGWDNGKTESFVYTEVDQTFAEGLGANGAWW
                     HRNQDRAGVAFVTNAIAKDHQRYLAAGGLGFLLGDGKLNYGREDVLESYYTAHVWRGL
                     YVGPGVQHINNPGYNRDRGPLLEPTFRLHTEF"
     gene            1466..2362
                     /locus_tag="VEJ00_11260"
     CDS             1466..2362
                     /locus_tag="VEJ00_11260"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HXX01787.1"
                     /translation="MRAFQTRCNGHSMVDPLRSVLVCSPLTAGWDRPRRTSTWRELGF
                     RHAPDFKIAQSQHHVLVRELETAGAEVVELPAADDLTLDAVYTHDASLATDFGLILMR
                     PGKPNRIAEAQRHGAFCQSLSIPTFATIAPPGTTEAGDMIWLDSKTLLIGHGYRTNAA
                     GIAQMRNLVAPKGVEVLSAPLPYGGGPSECLHLMSLISLLDDHTALVDLPWLAVETVE
                     LLKARGIRFIEIDHSERDTLACNVLALGNNRLLAIEENRNTNDRMRRAGFDVRTFPGS
                     EICINGSGGPTCLTRPLLRACS"
     gene            2434..>3662
                     /locus_tag="VEJ00_11265"
     CDS             2434..>3662
                     /locus_tag="VEJ00_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01788.1"
                     /translation="MRRSRSTLVLSVLLLFVIPTLVLAQTALIPRSVLFGNPARSSPE
                     ISPDGAMLAYLAPDAGVLNVWVRTLGKTDDRVITNDRKRGIRNFSWQFDSKNILYGQD
                     QGGDENWRIYQTNVATKQTKDLTPFENSRADIVALEPSRPDTLLIQSNKRDPKVFDVY
                     RVNLKTGELTLDTQNPGDVAGWQPDHDLQVRAAQVTTDDGGTIIRVRDDYKSPWRELT
                     KWGPDETLGNVDSFSPDNKALWIVTSLDANAARYISMDVATGKRTVIAEDPQFDVAFA
                     VTQPRTNKLQSVVFLRERRDFQILDPAIQADFDALRKVRDADISNISRDLKDDKWVVT
                     FEGDNAPVYYYLYDRASKQATMLFSNRPELEKYKLAKVKPVQYKARDGMTIYGYLTTP
                     AGSDAKNLPMVVFPHGGP"
BASE COUNT          750 a         1237 c          963 g          712 t
ORIGIN      
        1 caacaccgaa gccatgttcc cgcacttcaa ggacacgcgc ttctggctgt ccggccaggc
       61 caacttcatt tttcaaacgc atcccgaatt ccacgcggcg tacagcgggg cgcacagctt
      121 cggcccgcac tacgagaagg ccacgtcgcg cgtgctgacc atgttcggag gaatccgctt
      181 caacaactcc accgagttcg tcggcgccct ggaagaagcc ggaggatccg cactcagcct
      241 cggcttcggc atggctggca acactgatct cgacatcgtg cgcaatccgc tgctgagcaa
      301 ggcgccctat ctcggccgcg gcatgttcca taaagtgttc gctctcagcg acgacaaggt
      361 cgagaatcag cgcaactacc tgtctctttt tgacgaactg ccgcgccgcc gcctcgaaat
      421 ccgcttcggc aaattcagta tgcccgactt cttcgatatc aattccgtcg gatccgatac
      481 ccacttccag ttcagcaatt ggacgatcga caacaacggc gcctgggact atgccgccga
      541 cacgcgcggc tataccgtcg gattcaccgc cgacttcgaa gaccgcaact ggggcttccg
      601 cttcggcgag gctctcatgc cgagagtcgc caatggcatc gatctcgtct ggaagccctg
      661 gcaggcccac gctgaaagct ttgaatacga actccgtcac ggattcctcc cgaagaaatc
      721 cggtgtcgtc cgcctgcttg ccttcaccaa ttacgccaac atgggcatct accgccagca
      781 gctcgcacta gccgaacagc agggaacgac tcccgatatc accaatcatc cctggcacat
      841 cacacgcaag tacggcttcg gcgtcaacct cgagcagaat ctcacgcact acctcaccgc
      901 cttcgctcgc ttcggttggg acaacggcaa gaccgaatcg ttcgtttaca ccgaggttga
      961 ccagactttc gcagagggcc tgggtgcaaa cggcgcctgg tggcatcgca accaagaccg
     1021 cgctggcgtc gcctttgtca ccaacgcaat cgccaaagat caccagagat acctggctgc
     1081 cggcggcctg ggcttcctgc tgggcgacgg caagctgaac tacggccgtg aagatgttct
     1141 ggagtcctac tacactgccc acgtctggcg cggcctctac gtcgggcccg gcgttcagca
     1201 catcaacaac cccggctaca accgcgatcg cggcccgttg ctcgagccta ccttccgcct
     1261 gcacaccgag ttctagaggt tctgcccaaa aatacacacg cgggagggca ggcgccgagc
     1321 gtaaacgtgg aagagcggcc cttcagagcc tgccctgagc ttgtcgaagg ggccgcgtca
     1381 gcgcgtacaa tgcgttgggc ttcagcccca gtggtcgctt tttgtttaca acggaataat
     1441 ccgatcgaac cgaatccgca cgccaatgag agccttccaa acccgctgca atggccactc
     1501 catggtcgat cccctcagga gcgttctggt gtgctcgcct ctcactgcgg gctgggatcg
     1561 gccgcggcgg acctccactt ggcgcgagct aggattccgt cacgcacctg atttcaaaat
     1621 tgcacaatcc cagcatcacg tcctcgttcg cgaactggaa acggcgggcg cagaagtggt
     1681 cgaactccct gccgccgacg atctcaccct tgacgccgtc tacacgcacg atgcctctct
     1741 cgccaccgac ttcggcttga tcctcatgcg gcctggaaag ccgaatcgca tagccgaagc
     1801 ccaacggcac ggagcgttct gccagtcgct gagcattcca accttcgcta cgatcgctcc
     1861 gcccggaacc actgaggccg gcgacatgat ctggctcgat tcgaaaaccc tgctcatcgg
     1921 ccacggctac cgcacgaacg ccgctggcat cgcgcagatg cgaaacttag ttgcacccaa
     1981 aggagtggaa gttctctcag ctccgctgcc ttatggcggc gggccttccg agtgcctgca
     2041 cctgatgtcg ctgattagcc tgctcgacga tcacaccgcg ctcgtcgacc tgccttggtt
     2101 ggccgtcgag acggtcgaac tgctgaaggc gcgcggcatc cgcttcatcg agatcgacca
     2161 ttcagagcgc gatacgctcg cctgcaacgt cttggcgttg ggaaacaacc gcctgttggc
     2221 catcgaagag aatcgcaaca ccaacgaccg catgcgccgc gccgggttcg acgttcgcac
     2281 cttccccggc agcgagatct gcatcaacgg cagcggcggc ccgacgtgcc tcacgcgccc
     2341 cctgctccgg gcatgttcct gatcggtgga actccctcgg cttgtttctc gtattgaccc
     2401 ctaggtagaa tttgtctccc acggaggttg cccatgcggc gttcccgttc gaccctcgta
     2461 ttgtccgttc tccttctgtt tgtcattccc acactagttc tggcgcaaac cgccttgatc
     2521 ccccgtagcg tattgttcgg caaccctgcc cgcagttctc cggaaatttc tcccgacggc
     2581 gcgatgctgg cctatctcgc gccagacgcg ggggttctga atgtctgggt gcgcacgctg
     2641 ggcaagactg acgaccgcgt cattactaat gaccgcaagc gaggcatccg caatttttcc
     2701 tggcagttcg attcgaagaa cattctttat ggccaggatc agggcggcga cgagaactgg
     2761 cgtatctatc agaccaacgt tgccacgaag cagaccaaag atctcacgcc gttcgagaac
     2821 tcccgcgccg acattgtggc gctcgaacca agtcgtcccg atacgctctt gatccagagc
     2881 aacaagcgtg accccaaagt tttcgacgtc tatcgtgtga atctcaagac gggtgagctc
     2941 accctcgaca cccaaaatcc tggcgacgtt gccggctggc agccagacca tgacctccag
     3001 gtgcgcgccg cccaggtcac aaccgatgac ggcggcacca tcatccgtgt ccgtgacgac
     3061 tacaagtctc cctggcgcga actgaccaag tggggccccg atgaaactct gggcaacgtg
     3121 gacagcttct cccctgacaa caaggctctt tggatcgtca cttccctcga cgccaacgct
     3181 gcgcgctaca tctctatgga cgtcgccacg ggcaagcgca cggtgatcgc agaagacccg
     3241 cagttcgacg tcgctttcgc cgtcacccag ccccgcacca acaaattgca gtctgtcgtc
     3301 tttctccgtg aacgccgcga cttccagatc ctcgaccccg ctatccaagc tgacttcgat
     3361 gccctgcgca aggttcgcga tgccgacatc agcaacatca gccgcgatct gaaggacgac
     3421 aagtgggtcg tcacctttga aggcgacaac gctccggtct attactacct ctacgaccgt
     3481 gcgtccaagc aggcgacgat gctgttcagc aaccggcccg aactcgagaa atacaaactc
     3541 gccaaagtga agcccgttca atacaaggcg cgcgatggca tgacgattta cggttatctg
     3601 accacccccg ccggcagtga cgcgaagaac ttgccgatgg tcgtcttccc ccatggtggt
     3661 cc
//
LOCUS       DASPUL010000474         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_638956, whole genome shotgun sequence.
ACCESSION   DASPUL010000474 DASPUL010000000
VERSION     DASPUL010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638956"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..766
                     /locus_tag="VEJ00_11270"
     CDS             41..766
                     /locus_tag="VEJ00_11270"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXX01789.1"
                     /translation="MRATEFEFRNRWWVIFAIFFAAFGAYNIDHVSSAQAIVGWVARR
                     AGITAADNDLRILFAVGALFLVLAAFLRTWGTSYLRSEVMRDRQVHTERLLADGPYRY
                     VRNPLYLGNIFMAMGIGLMASRTGFVILVLGMTIFVIRLLLREEAELARDQGEPYRNY
                     CAAVPRLVPAISPQVPPAGDVPRWAQGIRAELMYWIMALAMAVFAVTLNIRLFWGLFA
                     VAMATAFLYKRPAPKVHPPPAAS"
     gene            complement(1110..>2198)
                     /locus_tag="VEJ00_11275"
     CDS             complement(1110..>2198)
                     /locus_tag="VEJ00_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXX01790.1"
                     /translation="TYLTAKWVFDLKDEDTYWCTADIGWVTGHSYIVYGPLQNGATSL
                     MYEGAPNFPEPDRFWSIIARHKVNVFYTAPTAIRTFIKWGDEWPKKHDMSSLRLLGTV
                     GEPINPEAWMWYRETIGGNRCPIVDTWWQTETGHIMLTPIPGAIATKPGSATRPFPGV
                     VPEIVTMDGKPVPDGSGGFLVLKRPWPGMLRTVYGDPDRYVKNYWSQIPGMYFTGDGA
                     RKDKDGYFWIMGRVDDVLNVSGHRLSTMEVESALVAHDAVAEAAVVGRPDDLKGQAIS
                     AFVTLELGRQPSNELKEELRKWVAKEIGALAKPDDIRFTDTLPKTRSGKIMRRLLREL
                     ATSGEVKGDTTTLEDFSVISKLKEHEEV"
BASE COUNT          368 a          701 c          640 g          489 t
ORIGIN      
        1 tcctgtcttc ggcagttctt ctgtggtagg ctagcccgcc atgcgtgcca ctgagttcga
       61 attccgcaac cgctggtggg tgatcttcgc catcttcttc gccgccttcg gcgcttataa
      121 catcgatcat gtgagctccg cgcaagcgat cgtagggtgg gtcgcgcggc gcgcgggtat
      181 cacagctgcc gacaacgacc tgcggatcct cttcgctgtg ggcgcgctgt ttctggtgct
      241 ggctgcattt ttgcgaacct gggggacctc ctatcttcga tcagaggtta tgcgagaccg
      301 ccaggtgcat accgagaggc tgctggccga cggcccctac cgttatgtgc gcaacccgct
      361 ttatctcggg aatatcttca tggcgatggg catcggtttg atggccagcc gcaccggatt
      421 cgtcatcctg gtgctgggca tgacgatctt cgtcattcgc cttctgctgc gcgaagaagc
      481 cgagctcgcc cgcgatcagg gcgagcccta tcgcaactat tgcgccgccg ttccccggct
      541 cgtgcccgcg atctctccgc aagtgcctcc cgccggagac gtgccgcgct gggctcaagg
      601 cattcgcgcg gaattgatgt actggatcat ggcgctggcc atggccgtat tcgccgttac
      661 gctgaatatc aggctcttct ggggattgtt tgccgtagct atggctacgg cgtttctgta
      721 caaacgcccg gctccgaaag tgcatcctcc gccggccgca agctgacctt cactgacagt
      781 gcgctgtaga aaatgcctga gggctgacgg ccgaatgtga aacgctacgt cgccttggcc
      841 tctgttcgac gacttggagc cttgtaatgc acccccactc ctcttgggga ggtgcgtggg
      901 ggtggcagcg ttcgggcaag gggtctcaat cccgcaccga accgtcgatc agcaatgcat
      961 tgcaccggaa ggtgagcacg tctcaggcca tcgcaagaac gcgctggtct gttgaaagct
     1021 ttggtccctg agaactgccc gctctttagc agtgtccact tgcaaatttc gtgtccgcag
     1081 gcggacgagc tgactgtcaa ctgcacggac taaacttcct cgtgctcctt cagcttggag
     1141 atcacgctga aatcttcgag ggtggtcgtg tcgcctttca cttcgccgct ggtggccagc
     1201 tcgcgcaaca ggcggcgcat gatcttgccg ctgcgcgttt ttggtagcgt gtcggtgaag
     1261 cggatatcgt ccggcttggc cagcgcgccg atttccttcg ccacccactt gcgcagttct
     1321 tctttcaact cgttcgaagg ctgccttccc agctccagcg tcacgaatgc tgaaatcgcc
     1381 tggcccttca ggtcatcggg acggcccacc accgcagcct cggccaccgc atcgtgtgcc
     1441 accagagctg actcaacttc catcgtgctc aatcggtggc cgctgacgtt gagcacgtca
     1501 tcgacgcggc ccatgatcca gaaatatccg tccttgtctt tgcgcgcgcc gtcgcccgtg
     1561 aaatacatgc cgggaatttg cgaccagtaa ttcttcacat agcggtcggg atcgccgtac
     1621 accgtgcgca gcatgcccgg ccacgggcgc ttgagcacga ggaatccgcc ggaaccgtcg
     1681 ggcaccggct tgccatccat ggtcacgatc tccggcacca cgcccggaaa cggccgagtc
     1741 gcagagcccg gcttggtcgc gatcgcgccc ggaatcggcg tcagcatgat gtgcccggtc
     1801 tcggtctgcc accacgtatc gacaatcggg cagcggttgc cgccgatggt ctcgcgatac
     1861 cacatccacg cttccggatt gattggctcg ccgactgtgc ccagcaggcg caggctcgac
     1921 atgtcgtgct tcttcggcca ctcgtcgccc cacttgatga aggtgcggat cgccgtcggc
     1981 gccgtataga acacattcac cttgtgccgc gcgatgattg accagaagcg gtcgggctcg
     2041 ggaaaattcg gtgcgccctc gtacatcagg ctggtggccc cattctggag cggtccgtac
     2101 acgatgtagc tgtgccccgt cacccatccg atgtcggccg tgcaccagta cgtgtcttca
     2161 tccttcagat cgaacaccca ctttgctgtg agatacgt
//
LOCUS       DASPUL010000475         8636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1184086, whole genome shotgun sequence.
ACCESSION   DASPUL010000475 DASPUL010000000
VERSION     DASPUL010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8636)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8636
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184086"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VEJ00_11280"
     CDS             <1..203
                     /locus_tag="VEJ00_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01791.1"
                     /translation="EPTVLVFKDGEKLEVGNYAIVGATLFDLSPGHHRKITLADLDLD
                     ATQKLNDDRGVIFQLPASSQAN"
     gene            206..856
                     /locus_tag="VEJ00_11285"
     CDS             206..856
                     /locus_tag="VEJ00_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01792.1"
                     /translation="MHRLIVVALLAAVPPWMSAQRAPAQHPVPATQPVRHTRPFFYPS
                     LFVPDVFYPDVFSTEEYSGASQPPVILLQAPPASNPAREPVPSPAEPLVIELQGDRYV
                     RLSGDDTSQTGMIERNPVPLRRPEKASSRSSQAVAPHELPPVILVFRDSHREEVSDYI
                     ITDGVLYARADYYAGGSWSKKIALTSLDLAETLDLNRSRGVAFRLPSAPNEVSTRP"
     gene            1068..3734
                     /gene="gyrA"
                     /locus_tag="VEJ00_11290"
     CDS             1068..3734
                     /gene="gyrA"
                     /locus_tag="VEJ00_11290"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXX01793.1"
                     /translation="MPDDQNPELPLTPPPGGDGPGALNIQPVNIEEEMKRSYLDYSMS
                     VIIGRALPDVRDGLKPVHRRILYTMQQMGLQPNRATRKCARIVGDVMGKYHPHGNLAV
                     YDALVRLAQDWSMRYPLIFGQGNFGSIDGDPPAADRYTEAKLAQVSVPLLEDLDKETV
                     DFGPNYDGSEVEPDVLPARIPNLLINGSDGIAVGMATKIPPHNLTEIVDATITLVNNP
                     NAQLAEILHFVQGPDFPTAGIIHGRAGIFEAYRTGRGRFMMRAKAAIENITKDRQAII
                     VTEIPYQVNKAKLVERIAQLVNDKDIEDISDIRDESDREGMRVVVELKRGVEPQIVLN
                     QLFKHTQMQESFSMILLAVVNGQPREMGLIQAIKYFIEHRVDVVRRRTAYLLAKAKDR
                     EHILEGYLTALDHLDNVIAIIRGSANRADARENLVLYFGGKKIDINTTGRAPKLDPEK
                     PFTSKQADAILELQLHRLTKLSIDEISKELTDVRGNIAEYESILGSEKKLRAVIIKEL
                     EEVKKQYGDERRTRIEDEAAEIVLEDLIADEQVAVTVSHSGYLKRTPISTYRMQRRGG
                     TGRTGMKTRDEDFVEHLFIVSTHAYILIFTNNGRVYWLKVYEIPDVSAAGKGKHIGNL
                     VGLQPGETLKTVVAVRNLEEENKYIFFATRNGLVKKSELREYMHVRSNGINAINIEQG
                     DELVAARITDGSQIIFLASHEGMAVRFDESHVRPMGRAAYGVWGIDLEDKDYVVGMAT
                     TPKPPADVGTAASAVQGAQAPAKEGSEPTDEAVAGSLILSVTENGFGKRTPADEYRLI
                     NRGGKGVVNVKTTPRTGNVVAIAQVDENSQVMLISQYGKIIRMDAATIRETGRNAQGV
                     RLLQMEPGDRVAAAVVIAPEEEGNGNGGTLIQ"
     gene            complement(3752..4489)
                     /locus_tag="VEJ00_11295"
     CDS             complement(3752..4489)
                     /locus_tag="VEJ00_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01794.1"
                     /translation="MAIYRVEPKPGEEPHKWKVEKFAEIGWNETFVRHVTELCTIVDA
                     SLIFDQQRADAKSAIMTILTDGLMPAFSELQKIRALEGKQLPLMDRRETYHDFCRKLW
                     KAYKDLTQRAATEMDFNIGFVFKNDKDFAKGLKEFQVNYPNVRPQLGSSLEEVRDRWQ
                     NELAKFRNTFLEHQDSDPKHFAKCYQLGYVESLFEEVWNTIVDLLAVLLESRLPHGTN
                     LALPDLSKNPKWPNRFVFDHPAFRNMT"
     gene            4636..5046
                     /locus_tag="VEJ00_11300"
     CDS             4636..5046
                     /locus_tag="VEJ00_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01795.1"
                     /translation="MDRAPLFTLARIVLLDNLTLYQVEDNMVQEQGVIPKPLTEQELK
                     KLVVDAINATTYAETLETYHALYGHMERGIGTDDVIHGLESEWQFERPPEFNQKYWQW
                     KYYIATKTVDEVPITVVIAVDSWRREFKTVTRWR"
     gene            5007..5576
                     /locus_tag="VEJ00_11305"
     CDS             5007..5576
                     /locus_tag="VEJ00_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01796.1"
                     /translation="MATRIQNGDEMAIDQTHECTSSSRRELIATEEEPYHFIESGLPN
                     IYLVGVRYFECECGERFVEIPAIKQLMALIARHAVMKEQALTGTEIRFLRKRLGQKAA
                     DFASAVKLQPETLSRVENEKQSVGQKTDFYIRIYYAFASKDPVLLDALKQALDKALSM
                     RRSKPPKKLPKTVAKIEHDEWALAAGAGR"
     gene            complement(5748..6737)
                     /locus_tag="VEJ00_11310"
     CDS             complement(5748..6737)
                     /locus_tag="VEJ00_11310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXX01797.1"
                     /translation="MKMRELGRSGLNVSAQGLGCMGMSEFYGGRDDQESIATIHRALE
                     LGITFLDTADVYGPYKNEELVGKAIKGKRDKVVLATKFGIVRDPNDPMRRGVSGKADY
                     VQRACEASLKRLGVETIDLYYQHRVDPKTPIEETVGAMAELLKQGKVRYLGLSEASPT
                     TLRRAAKVHPIAALQTEYSLWTRDPENGVLAACRDLGIGFVAYSPLGRGFLTGQFRRF
                     EDLPADDYRRFTPRFQGENFQKNLDLVRQVEEIAREKGCKPSQLALAWVLAQGNDIVP
                     IPGTKRRKYLEENAAADAIQLTSDDLRRIDEVFPPEAAAGKRYPEHMMDLVNG"
     gene            6884..7399
                     /locus_tag="VEJ00_11315"
     CDS             6884..7399
                     /locus_tag="VEJ00_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX01798.1"
                     /translation="MSRVSAWFERKFDFSLPVQLYPNLLTRLRGTPARLEELVRGRPM
                     ETMVRKENGKWSAQEHAGHLGDLEPLWLARVQDFTTGGAELTAADLKNQKTHSANHNA
                     HPVEEILADFRRKRAALLHRVENAAPEMFGCTMLHPRLKQPMRLVDHLYFVAEHDDHH
                     LAQIWELINKG"
     gene            complement(7495..8589)
                     /gene="selD"
                     /locus_tag="VEJ00_11320"
     CDS             complement(7495..8589)
                     /gene="selD"
                     /locus_tag="VEJ00_11320"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8536..8538),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HXX01799.1"
                     /translation="MPETKPFRLTESVKAAGUASKLSPAALDKVLGKLARQQDPNVLV
                     GFDHADDAGVYQIAPDQALVQTVDFFTPIVDDPYTFGQIAATNSLSDVYAMGGRPLTS
                     LALVCFPDKADLEILERILAGGLSKMIEAGCTVIGGHSIRDEETKFGYSVTGLVHPKK
                     VLANAGAKPGDALLLTKALGTGVISTAIKKGKAEQAWIDGAVASMTTLNKRAAEVITT
                     GHVETAAPGRPTEQSSAGFGVNAMTDVTGFGLIGHLREMLLASEVSAQISGGSIPRLD
                     GALECIRAGHIPGGLKNNRDFAECLVEYDKRVQEDVKALLYDPQTAGGLLIATPEGAR
                     LESALNDSGVTAVQIGEVVTEGKPRIRVSM"
BASE COUNT         1903 a         2724 c         2261 g         1748 t
ORIGIN      
        1 aagagccaac cgtgctcgtg ttcaaggacg gcgagaagct ggaagtgggg aactacgcca
       61 tcgtgggtgc gacgcttttt gatctctccc ccgggcatca ccgcaagatc acgctcgccg
      121 atctcgatct cgatgccacg cagaaactga atgacgaccg cggcgtgatc ttccagctgc
      181 cggcatcgtc acaggcaaac tagcgatgca taggctcatc gtcgtcgcgc tgctggctgc
      241 ggtcccgccg tggatgagcg ctcagcgagc gcccgcccag cacccggtgc ccgctacgca
      301 gcctgttcgc catactcggc cttttttcta tccgtccctc tttgtgcccg acgtgttcta
      361 cccggatgtt ttctccaccg aagaatattc cggcgcttct caaccgcctg taattcttct
      421 gcaggcccca cccgcgtcaa acccggctcg agagcccgtt ccatcgccgg ctgagccgct
      481 cgtgatcgaa ctgcaggggg accgctacgt tcgcctgagc ggtgacgata cttcgcaaac
      541 cggcatgatc gagaggaacc ctgtcccgct ccgccgtccg gaaaaggcat cgagcagatc
      601 gtcgcaggcg gtcgcgcctc atgaactgcc gccagtgatt ctcgtgttcc gcgacagcca
      661 tcgggaggaa gtctccgact acatcatcac cgacggagtg ctctacgccc gcgccgacta
      721 ttacgccgga ggatcgtgga gcaagaagat cgcactcacg tcgctcgacc tcgccgaaac
      781 tctggatttg aatcgctctc gcggcgtggc cttccgcctc ccctcagccc cgaatgaagt
      841 gagcaccaga ccatagttcg gttcgggaaa aatagctgca cgcgcctcaa tctcgtcaac
      901 ccaagcctcc ggcgaagccc catttcgtga tatactgtga gcagttcccg ggactccaac
      961 atcctgtaaa tacaatcagt tacgaggcac cactggccgg atgagtgccg cctcgactgg
     1021 tgaagtgaac tcgtggctgc gaaccgggag ctaaccaaga gctgaccatg cctgacgacc
     1081 agaacccaga gcttcctctt actcctcctc ccggcggtga cggccccggg gcgttgaaca
     1141 ttcaaccggt caacattgaa gaggagatga agcggtcgta tctcgactat tccatgtcgg
     1201 tgatcatcgg ccgcgcgttg cccgacgtgc gcgacggatt gaagcccgtg catcgccgca
     1261 ttctctacac gatgcagcag atgggcctgc agcccaaccg cgccacgcgc aaatgcgccc
     1321 gcatcgtcgg cgacgtcatg ggcaagtacc acccccacgg caatctcgcc gtttatgacg
     1381 ctctcgtccg cctcgcgcag gactggtcaa tgcgctatcc gttgatcttc ggccagggga
     1441 atttcggctc gattgacggt gatcctcccg cagccgaccg ttataccgaa gccaagctgg
     1501 cgcaggtctc ggtcccgcta ctcgaagacc tcgacaaaga aacggtcgat ttcggcccca
     1561 actacgacgg cagcgaagtc gagcccgatg ttcttcccgc gcgtattccg aatctgctca
     1621 tcaacggctc tgacggcatt gcggtcggca tggccacaaa aattccgcca cacaatctga
     1681 ccgagatagt cgacgccacc atcacgctgg tcaacaatcc caatgcgcaa ctggccgaga
     1741 ttctgcactt cgtgcagggc ccggattttc cgacagccgg catcattcac ggccgcgcgg
     1801 gaatcttcga ggcctaccgg accggccgcg gccgcttcat gatgcgcgcc aaagccgcta
     1861 tcgagaacat caccaaagat cgccaggcca tcatcgtcac cgagattccg tatcaggtga
     1921 acaaggccaa gctggtcgag cgcatcgcgc aattggtcaa cgacaaagac atcgaagata
     1981 tctccgacat tcgcgacgag agcgatcgcg aaggcatgcg cgttgttgtt gaactcaagc
     2041 gcggagttga accccagatc gtcctcaatc agctcttcaa gcacacccaa atgcaggaga
     2101 gtttctcgat gatcctgctc gcggtggtga acggacagcc gcgcgagatg ggcctgatcc
     2161 aggccatcaa gtatttcatc gagcaccgcg tcgacgtcgt ccgccgccgc accgcctacc
     2221 tgctcgccaa ggcgaaagac cgcgagcaca ttctggaagg ctacctgacc gcgctcgatc
     2281 acctcgacaa cgtgatcgcc atcattcgcg gcagcgccaa ccgtgccgac gcccgcgaaa
     2341 acctggtgct ctacttcggc ggcaagaaaa tcgacatcaa cacaaccggc cgcgcaccca
     2401 agctcgatcc cgaaaagccg ttcacgtcca agcaggccga cgccatcctc gaactgcagt
     2461 tgcaccgcct gaccaaactc tcgattgacg agatttccaa agaattgacc gacgtccgcg
     2521 gcaacatcgc cgaatacgaa tcgattctcg gcagcgagaa gaagctgcgc gccgtcatca
     2581 tcaaggaact cgaagaagtc aagaagcagt acggcgatga gcgccgcacg cgcatcgaag
     2641 acgaagctgc ggaaatcgtc ctcgaagatc tcatcgccga tgagcaggtt gccgtcacgg
     2701 tcagccactc cggctatctc aagcgcacgc ccatctcgac ctaccggatg cagcgccgcg
     2761 gaggcacagg ccgcaccggc atgaagacgc gggacgaaga ttttgtcgag catcttttca
     2821 tcgtttccac acacgcctac attctgattt tcaccaacaa cggccgcgtc tattggctca
     2881 aggtctacga aattcccgat gtcagcgccg ccggcaaggg caagcacatc ggcaacctgg
     2941 tcggcttgca accgggcgaa acgctgaaga cggtcgtagc cgtgcgcaac ctcgaagaag
     3001 aaaataaata catcttcttc gccacccgca acggcctggt gaagaaatca gagctgcgcg
     3061 agtacatgca cgtgcgctcg aacggcatca acgccatcaa catcgagcag ggagacgaac
     3121 tcgtcgccgc ccgtatcacc gacggcagcc agatcatctt cctcgccagc cacgaaggca
     3181 tggccgtccg tttcgacgaa agccacgttc gccccatggg ccgcgctgcg tacggcgtct
     3241 ggggaatcga tctcgaagac aaggactacg tcgtgggcat ggcaaccacg ccaaagcccc
     3301 cggcagatgt agggacagcc gcctcggctg tccagggcgc gcaagcgccc gcaaaggaag
     3361 gtagcgagcc cacggacgaa gccgtcgccg gctcgctgat cctctccgtc accgaaaacg
     3421 gattcggcaa gcgcactccc gccgacgaat accgcctcat caatcgcggc ggcaaaggcg
     3481 tggttaacgt taagaccact ccccgcactg gcaatgtcgt tgccatcgcg caggtcgacg
     3541 agaactcgca ggtcatgctg atcagccagt acggcaagat catccgcatg gacgcggcca
     3601 ccatccgcga aaccggccgt aacgcccagg gcgtccgcct gctgcagatg gagccaggcg
     3661 accgggtagc agccgccgtc gtgatcgcgc cggaagaaga aggaaacggc aacggcggga
     3721 cgctgattca gtaaggagtg ttgcaaagaa gctacgtcat gtttctaaag gcaggatggt
     3781 cgaagacaaa gcgattcggc cattttggat tcttgctcag gtcagggagt gcgaggtttg
     3841 tgccgtgcgg caaccgactt tcgagtaaaa ctgctaatag atcgacgatt gtgttccata
     3901 cctcttcgaa gagggattcg acatagccaa gctggtagca cttcgcgaaa tgtttcggat
     3961 cgctatcttg gtgttccagg aaagtgttgc gaaacttggc aagttcattc tgccaacgat
     4021 ccctgacttc ctcaagagaa ctgcccaatt gagggcgcac gttcggataa ttcacttgga
     4081 attccttgag gccctttgcg aagtccttat cgttcttaaa cacaaatcca atattgaaat
     4141 ccatctcggt cgccgcccgt tgagtgaggt ccttgtacgc cttccatagt tttcggcaga
     4201 agtcatgata ggtctcgcgc ctgtccatga gcggtaactg cttcccttca agggcgcgga
     4261 ttttttggag ttccgaaaac gccggcataa gcccgtcagt caagatcgtc atgatggcgc
     4321 ttttggcgtc agcgcgctgc tggtcgaaga tgaggctcgc atcgacgatc gtgcacagtt
     4381 ctgtcacatg ccgtacgaac gtctcgttcc agccaatctc cgcgaatttc tcgaccttcc
     4441 atttgtgcgg ttcttcgccc ggtttcggct ctactcggta aatggccatc aacccactcc
     4501 tgacagcatc ttcagggaac tttccgactc gaattcgccg ccaatttcga acgcgctacg
     4561 acttcggaaa cagtaccggt agctggccgg ccccacagct gagatacaat tccttgtgcg
     4621 ttgcacggga tgcggatgga ccgcgctccc ctttttaccc ttgcaaggat tgttttgcta
     4681 gacaacttga cattatatca agtggaagat aatatggtcc aagaacaagg ggtcatccct
     4741 aaacccctga ctgagcaaga acttaagaag ctagtcgtcg atgcgattaa cgctactacc
     4801 tacgccgaga cgctagagac gtatcatgcg ctatacggtc atatggagcg cggcatcgga
     4861 acggatgacg tgattcatgg attggagagc gaatggcagt tcgagcgacc ccctgaattc
     4921 aatcaaaaat attggcagtg gaaatactac atcgccacaa aaacggttga cgaggtcccc
     4981 atcacagttg tcatagccgt cgattcatgg cgacgcgaat tcaaaacggt gacgagatgg
     5041 cgatagacca aacgcacgaa tgtacatcta gttcgcgacg agaactcatc gcgacagagg
     5101 aagagcccta tcactttatc gaatccggcc tgcctaatat ctacctagtc ggcgttcgct
     5161 atttcgaatg tgagtgcggt gaaagattcg ttgaaattcc ggcaatcaaa cagcttatgg
     5221 ccctgatcgc ccgtcatgcg gtgatgaaag agcaagcatt gaccggaacg gaaatcagat
     5281 tcttacgcaa gcgcttggga caaaaggctg ccgattttgc aagcgcagtt aaacttcaac
     5341 ccgagacatt atctcgcgtc gaaaacgaga aacaatctgt tggacaaaaa actgatttct
     5401 atatacgcat ttattatgca tttgcttcta aagacccggt cttgctagat gctttaaagc
     5461 aagctctaga taaggccctg tcaatgcggc gttctaaacc tcccaagaag ttgccgaaaa
     5521 cggttgcaaa gattgaacat gacgagtggg cgctagctgc aggcgcgggg cgatgaaccc
     5581 tttccaccca caacccgtgt ttctgacctt tcgtccgccc gttcagcagc acgatcatgt
     5641 ggtatttgac atcttttctt gcgcttggcc cgaatcgtgt aatttctccc tattgacaag
     5701 atttttctga taacccgctg tcttgcggaa gcttcgccct cggcgtttca cccattcacc
     5761 agatccatca tgtgctccgg atagcgcttc cccgcggccg cttccggcgg aaacacttcg
     5821 tcaatccttc gcaagtcatc cgacgtgagc tgaattgcat ccgccgctgc gttctcttcc
     5881 agatacttgc gcctcttcgt gcccggaatc ggaacaatgt cgttcccctg tgccaagacc
     5941 caggccagcg ccagctgcga aggcttgcag cctttctcgc gcgcgatctc ctccacctgc
     6001 cgcaccaggt ccagattctt ctgaaaattc tccccctgaa accggggcgt gaagcgccgg
     6061 tagtcgtccg ccggcaaatc ttcaaatcga cggaactgtc ccgtcaaaaa gccgcgcccc
     6121 agcgggctgt aggccacaaa cccgatcccc aagtcgcggc acgcagccag cacgccgttc
     6181 tcaggatcgc gcgtccacag cgaatactcc gtctgcaggg ccgcgatcgg atgcaccttc
     6241 gccgcccgcc gcagcgtcgt tggcgaagcc tccgacagcc cgagataccg gaccttgccc
     6301 tgcttcagca gctcggccat cgcgccgaca gtttcttcaa tcggcgtctt cggatcgacc
     6361 cgatgctggt agtacagatc gatcgtctcg actcccagcc gcttcaggct ggcctcgcag
     6421 gcccgttgca catagtcggc cttgccgctg acgccacgcc tcatcggatc attcgggtcg
     6481 cggacaatcc caaacttcgt cgccagcaca actttgtcgc gcttgccctt gatcgccttg
     6541 ccgaccagct cttcattctt gtacggaccg taaacatcgg ccgtgtcgag gaacgtaatc
     6601 cccagttcca gcgctcggtg aatcgtcgca atcgactcct ggtcgtcgcg tccaccgtaa
     6661 aactccgaca tgcccatgca ccccaatccc tgggcggaaa cgttcagacc gctacggcca
     6721 agttcacgca ttttcatggc tggctcctgc aaagctttcg caacaacgcg gcttgtttag
     6781 atgccaaagt gcggcttgtg ctgcatgaat ccgtgagtcg ccagcttcat tcgttgcatc
     6841 ttgggactga acgaccattc ccaggattac ttccggagga atcatgagca gggtatccgc
     6901 ctggtttgag cgcaagttcg acttttcgtt acccgtgcag ctttatccaa acctgctgac
     6961 ccgattgcgc ggcactcccg cgcgcctgga ggaacttgtg cgcggccggc ccatggaaac
     7021 aatggtcagg aaggaaaatg gaaagtggtc cgcccaggag cacgccggcc acctcggcga
     7081 tctggagccg ctttggctgg cacgcgtgca agatttcacg accggcgggg ctgaactcac
     7141 ggcagcggac ttgaagaatc agaagaccca cagcgccaat cacaacgctc atccggtgga
     7201 agaaatcctc gcagacttca gaaggaagag agccgcgctg ctgcatcggg tcgagaacgc
     7261 cgcccctgag atgttcggct gcaccatgct tcacccccgc ctgaagcagc ccatgcggtt
     7321 ggtagatcac ctgtacttcg tcgccgagca cgacgatcac cacctggctc aaatctggga
     7381 actgatcaac aaaggctgac gcacatcgga acgatcctct tttcatcggt gtcatctcga
     7441 agcctcgcgc cttaaccggc gaggcgaggg atctcgcgcg gttcttcatg tgcgttacat
     7501 cgaaacccgt atcctcggct ttccctccgt gacgacctcc ccgatctgca cggccgtcac
     7561 tcccgaatca ttcagagcac tctcgagcct cgcaccctcg ggtgttgcaa ttagcagtcc
     7621 gcctgcagtc tgcggatcgt agagcagtgc cttaacgtcc tcttgcacgc gcttgtcgta
     7681 ttccaccaag cactcggcaa aatcgcggtt gttcttgagg ccacccggga tatgtcccgc
     7741 ccgaatgcac tccaaagcac catccagtcg cggaatactt ccccccgaaa tctgcgccga
     7801 cacttccgac gccagcagca tctcgcgcag gtggccgatc aatccgaagc cggtgacatc
     7861 ggtcatcgcg ttcacaccaa aaccagcgga gctttgctcc gtcggacggc cgggggcggc
     7921 cgtctctaca tggccggttg taattacctc agccgcccgt ttgttcagcg tggtcatcga
     7981 cgctactgcc ccatcgatcc acgcttgctc cgccttgccc ttcttgatcg ctgtcgaaat
     8041 tacgcccgtg cccagcgctt tggtcagcag cagcgcatcc cccggctttg ccccagcatt
     8101 ggccaaaact ttctttggat gcaccaaccc cgtcaccgag tacccgaact tcgtctcctc
     8161 gtcgcgaata ctgtgcccgc caatcacggt gcagcccgcc tcgatcatct tcgaaagccc
     8221 gccagcgaga atgcgctcca gaatctctag atcggccttg tcgggaaaac aaaccagcgc
     8281 cagcgacgta agcggccttc cgcccatggc atagacatca ctcaaggaat tggtcgccgc
     8341 gatctgcccg aacgtgtacg gatcgtcgac gattggagtg aagaagtcga cggtttgcac
     8401 caacgcctga tcgggcgcaa tttgatacac gcccgcgtcg tcggcgtgat caaagccgac
     8461 cagaacgttc ggatcctgtt gccgggctaa tttcccaagc accttgtcca gcgccgccgg
     8521 actcagcttg ctcgctcaac ccgccgcttt caccgactcc gtcagccgaa atggcttggt
     8581 ttcaggcacg aggccattgt agaggattgg cttaggattt cagattttgt gattgc
//
LOCUS       DASPUL010000476         5849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_767449, whole genome shotgun sequence.
ACCESSION   DASPUL010000476 DASPUL010000000
VERSION     DASPUL010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767449"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VEJ00_11325"
     CDS             complement(<1..654)
                     /locus_tag="VEJ00_11325"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase catalytic subunit"
                     /protein_id="HXX01800.1"
                     /translation="MTTPSPDLSVSFAGIALKNPIIAASGTFGYGVEFEDVVHLNKLG
                     GLVVKGLSKEPMIGNPPPRLWETAAGMLNAIGLQNIGAQGFLDEKLPRLRQINDIVVI
                     ANVFGYTREDYERTIQILNQGEGIAAYELNVSCPNTTQGGLQFGSDPRSLDEVVTTAK
                     RVAERPLIVKLSPNVTSIAQMAYVAQEAGADALSLINTFVAMAIDPETRKPRIANITA
                     "
     gene            complement(651..2291)
                     /locus_tag="VEJ00_11330"
     CDS             complement(651..2291)
                     /locus_tag="VEJ00_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXX01801.1"
                     /translation="MRPSLGFAGSRVVCVFTFLAAFLSISTLSSASAYNGHPRLVVVI
                     VIDQFRGDYIERYRDQFSEDGFRLFLDHGAYFPDCNYNYANTRTAPGHSTLFTGAYTS
                     GHGIVANEWWDQKKKKMVTSVEDDSTKQVGVAGERTGASPHNLMADTLGDELKLATQG
                     KARVFGVSLKDRAAVLPAGFAGDTAYWIDPKSGAWITSTYYRDDLPRWVQDFNANQPA
                     KYWDRKWTDSQGRVLRTTEHRQGKDGSEAGFYGVVGPTAFGNDYEFEFAKQLVLFENM
                     GRGPATDLLSISLSPNDMLGHGVGPDSPEMRQMALDLDRQLADFFNFLRHQIGLAEVW
                     IALSADHGVSSLPDAVRKLRIPAANGGEKIEAQVNAALTSKFSPGHPATYVRLDYPLA
                     WLDQDAFAEVHVKEHDAEVAVGEAMKQSGLREYYTKSQLAEGEAPDTPLGRKFLNSYS
                     PEGSWYVMGVQDPYTVGPASGTDHASPYTYDTHVPLAFYGLPFQAGTYRTSVEPVDMA
                     ATLASLLGINAPTHSVGRVLTEALARQGATPGAGESRP"
     gene            2441..2869
                     /locus_tag="VEJ00_11335"
     CDS             2441..2869
                     /locus_tag="VEJ00_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXX01802.1"
                     /translation="MKKLVCCMTLLAMLLVPALMAQKKSGSKSAALIARGKYLVESTS
                     MCNDCHSPHNEKGEVVEAQRLKGGPLEFKPAVPIPVWADKAVNIAGLPGWEKDAAIKF
                     MMTGIAYNDLPARPPMPQYRFNQQDAEAIVAYLQSLAPAK"
     gene            3049..4383
                     /gene="serS"
                     /locus_tag="VEJ00_11340"
     CDS             3049..4383
                     /gene="serS"
                     /locus_tag="VEJ00_11340"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXX01803.1"
                     /translation="MLDLNFVRENLPLVEEKLRQRGLDPAAVLKNFREVDSQRRQAIT
                     EADGMKARRNVLVEAFQNLRQEEKQLKATGKDATANEVSRAENLAEQALIKKSMPRAD
                     AAVDDFNARLLEILAGIPNLPHESVPVGRSADDNVEVRRWGTPPKFDFTPKPHWDLGP
                     ELGILDLERAVKITGARFAVYWDLGAKLERALANFMLDLHTREHGYAEVLPPYLVNSD
                     SLYGTGQLPKFADDQFRVPHGERDLWLIPTAEVPVTNLYRDEVLDAARLPISLTAYTP
                     CFRSEAGSYGKDVRGIIRQHQFQKVELVKFTRPENSYEEHEKLTHNAEEVLQKLGLHY
                     RVVTLCTGDMGESSAKTYDIEVWLPGQQLFREISSCSNFEAYQARRANIRYRPEGKNK
                     TEFVHTLNGSGLAVGRTWVAVVENYQQADGSVVIPEALRPYIGADRITPKRF"
     gene            4485..4907
                     /locus_tag="VEJ00_11345"
     CDS             4485..4907
                     /locus_tag="VEJ00_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01804.1"
                     /translation="MHRAELTTAAPSAPLPMNAVWRTIRGYILWQYERGTLHYDIMVT
                     LILTFVLFSPKVINFNDKPIARNPHPTDVIVSTDAEGHLVYQVSASAISPGDDRAIRD
                     QLLRIIEPISGAVSIVSYETVADGKGKVQSYKVQVKRE"
     gene            4907..5620
                     /locus_tag="VEJ00_11350"
     CDS             4907..5620
                     /locus_tag="VEJ00_11350"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HXX01805.1"
                     /translation="MKAHATISVALLPMLAALTLLPQQSQNSPLDSVLKKMDETAASF
                     RTAQADFEWDRYEKVIDEVDDVQSGTIYYRRIGKDGKEVEMKADITKPDRKYVLFSGG
                     KIQMYLPKPDQLTVYDAGRNRSDFETYLVLGFGGSGHDLMKAFDVTYMGPETINGVAT
                     AKLQLVPKSEKVRGTFKQILLWIDLANGISEQQQFIEPQGDYRLAKYSSVQVNGKKIP
                     DDVFKLKTTSKTQTISPHG"
BASE COUNT         1227 a         1785 c         1699 g         1138 t
ORIGIN      
        1 ggcggtgatg ttggcaatac gcggcttgcg cgtctcggga tcaatcgcca tggctacgaa
       61 agtgttgatc agcgacagcg cgtcggcacc cgcctcctgc gccacataag ccatttgcgc
      121 gatgctggtc acgttcggcg agagcttcac gatcaacggc cgctcggcca cgcgcttggc
      181 ggtcgtcacc acttcatcga gcgatcgcgg atcgctgccg aactgcagcc cgccttgagt
      241 cgtgttcggg cacgaaacgt tcaactcgta agcggcgata ccttcgccct gattcagaat
      301 ctgaatggtg cgctcataat cttcgcgcgt atatccgaag acgttagcga tgacaacaat
      361 gtcgttgatc tgccgcagcc ggggcagttt ttcgtcgagg aatccctgcg ctccgatgtt
      421 ctgcagaccg atcgcgttca gcatgcccgc ggctgtctcc cacaaacgcg gcggcgggtt
      481 gccgatcatc ggctccttcg acaggccctt caccaccagc ccgcccagct tgttgaggtg
      541 caccacgtcc tcaaactcca cgccgtatcc gaaggtgccg ctggcggcaa tgatgggatt
      601 cttgagcgcg atgccggcga agctgacgct gagatcagga gagggagtcg tcatgggcgg
      661 ctttctccgg cgcccggcgt cgccccttgg cgggcgagcg cctccgtaag cacgcggccc
      721 acggagtgcg tcggggcgtt gatccccagc agcgatgcca gcgtcgccgc catgtcgacc
      781 ggttcgacgc tggtgcggta ggttcccgcc tggaaaggca gcccgtagaa cgccagcggg
      841 acgtgcgtgt cgtaggtgta gggcgaggcg tggtcggtgc cgcttgcggg accaaccgtg
      901 tagggatcct gtacacccat cacgtaccag ctgccctccg gcgaataact gttcaggaac
      961 ttccgtccca gcggcgtgtc cggcgcttcc ccctccgcca gttgcgactt agtgtagtac
     1021 tcgcgcaacc cgctttgctt catggcttcg ccgacggcaa cctccgcgtc gtgctctttc
     1081 acatgaactt cggcaaaggc gtcctgatcg agccaggcca gcggatagtc gagccgaaca
     1141 taggtcgcgg gatgcccggg cgagaatttc gaagtcagcg ccgcgttgac ctgcgcctcg
     1201 atcttctccc cgccatttgc ggcgggaatg cgcagcttcc tcacggcatc aggcagtgag
     1261 gaaacgccat gatcggctga aagcgcgatc cacacctcgg ccaagccgat ctggtggcgg
     1321 aggaaattga agaaatcggc caactgccgg tcgagatcga gggccatctg acgcatttcc
     1381 ggcgaatccg gaccgacacc atggcccagc atgtcattgg gcgaaaggct gatcgacaac
     1441 aagtccgtag ccgggccgcg gcccatgttt tcaaagagca ccagctgctt cgcgaactcg
     1501 aactcgtagt cgttgccgaa ggcggttggg ccgacaactc cgtagaagcc cgcctccgag
     1561 ccgtctttgc cctggcgatg ctcggtcgtg cgcaggactc ttccttggga atccgtccac
     1621 ttgcggtccc agtatttcgc cggctggttg gcattgaaat cctgcaccca gcgcggcagg
     1681 tcatcgcggt aataggtcga cgtaatccac gcgccgcttt tcggatcgat ccagtacgca
     1741 gtgtctccgg cgaacccggc gggcagcaca gcggcgcggt ccttcagcga aacgccgaac
     1801 actcgtgcct tgccttgcgt ggccagcttg agttcgtcac ccagggtgtc ggccatcaga
     1861 ttgtgaggag atgcgcccgt gcgttctccc gccacaccca cctgtttggt gctgtcgtcc
     1921 tccaccgagg tcaccatctt cttttttttc tggtcccacc actcgttggc aacaatgcca
     1981 tggccgctcg tataggctcc agtgaagagc gtggaatgtc cgggggctgt gcgggtgttg
     2041 gcgtagttgt agttgcagtc cggaaagtag gcgccgtgat cgagaaacag gcgaaacccg
     2101 tcttcgctga actggtcgcg atagcgctcg atgtagtctc cgcggaactg gtcgatgacg
     2161 atcaccacca ccagcctggg atggccgttg taggccgatg cagagctgag cgtagaaatg
     2221 ctgaggaacg cagccagaaa cgtgaacaca cacacaacgc gcgagccggc gaatccgagg
     2281 gatggtctca tcgcgaaggt agtttacaca gccggggaga ttccgccaaa ggcggtggcc
     2341 cctgacacaa acgattcagg gcggttctga atagccggaa gaggtgctaa gattctcccg
     2401 ccgtttgtat ccccctgttg aagtgaggag cccttcgtct atgaagaagc tagtgtgctg
     2461 catgaccctg ctcgcgatgc tgctggttcc cgccctgatg gcgcagaaga aatccggttc
     2521 caagtcagcc gcgctcatcg cccgcggcaa atatctcgta gaaagcacca gcatgtgcaa
     2581 cgactgccac tcgccccata acgagaaggg agaggtcgtc gaagcgcagc ggctcaaggg
     2641 cgggcctctc gaatttaaac ccgccgtacc cattcctgtg tgggccgaca aggcggtgaa
     2701 catcgcaggc ctccccggct gggaaaagga cgccgccatc aagttcatga tgaccggcat
     2761 cgcctacaac gacctgcccg cgcgcccacc gatgccgcag taccgcttca accagcagga
     2821 cgcggaggcc atcgtcgcct atctgcagtc gctggcaccg gcgaagtaag aggctgttga
     2881 tccggcgcta cctattggcg gggaaacgct acccgggccc cgtatgtttc tttgcgtcct
     2941 ctgcggactt tcctcggcgt tctctgcggt caaaagcttt gccgtaacga gcagcgccca
     3001 tccccacgtg cattcccctc tgcttttccg atagagtatt gagttcccat gcttgacctg
     3061 aatttcgtcc gtgagaatct gcctctggtc gaggagaagc tgcgccagcg cggcctggat
     3121 ccggctgccg tgctgaagaa cttccgcgag gtagactccc aacgccgcca ggcaattacc
     3181 gaagccgatg ggatgaaggc tcgacgaaac gtgttggtgg aagctttcca gaacctcagg
     3241 caagaggaaa agcagttaaa ggcgactggc aaggacgcga ccgcgaacga ggtttcccgt
     3301 gccgagaatc tggctgagca ggccctaatc aagaaatcga tgccgcgggc ggatgccgcg
     3361 gttgacgact tcaacgctcg tctgctggag attctcgcgg gtattcccaa tctgccgcat
     3421 gaaagtgtcc cggtcggccg cagtgccgac gacaatgtcg aagtccgccg ctggggaact
     3481 ccgcccaagt tcgacttcac gcccaagccc cattgggatc ttggtcccga gctgggcatt
     3541 ctcgatctcg aacgcgcagt gaaaataacc ggcgcgcgtt tcgccgtcta ctgggatctg
     3601 ggcgcgaagc tcgagcgtgc acttgccaat ttcatgctgg atctgcacac ccgcgaacac
     3661 ggctacgcgg aagttctgcc accgtatctc gtcaattcgg attcgctgta cgggacgggc
     3721 cagctgccca agtttgctga cgatcaattc cgcgttcccc acggcgaaag agatctctgg
     3781 ctcatcccca ccgccgaagt cccggtgacg aacctctatc gcgacgaagt cctcgacgcg
     3841 gcgcggctgc cgatctcgct taccgcctac acaccttgct tccggagcga agcgggatcg
     3901 tatggcaaag acgtgcgcgg catcatccgc cagcaccagt ttcagaaagt ggaactggtc
     3961 aaatttacgc gtccggaaaa ctcgtacgaa gagcacgaga agctgactca caacgccgag
     4021 gaagttctgc agaaactcgg gctgcactac cgcgtcgtca ctttgtgcac tggcgacatg
     4081 ggagaatcct cggcgaagac ttacgacatc gaggtctggc tgccgggcca gcaattattc
     4141 cgcgagatct cgtcatgctc gaacttcgag gcctatcagg cgcggcgcgc caatatccgc
     4201 taccgcccgg aaggcaagaa caagaccgag tttgtgcaca cgctcaacgg cagcggcctg
     4261 gctgtgggac gaacctgggt tgcggttgta gagaactatc agcaggccga tggcagcgtg
     4321 gtgattcctg aggcgctgcg gccgtatatc ggcgcagaca ggatcacgcc gaagaggttt
     4381 tgagccccga accatgagtt ttaagcgtca aagctcgcag ctcaaggctc atagctcgaa
     4441 gctcgcagct cgatcattgc caagacaagg accggtcact agacttgcat agagcagaac
     4501 tcacgactgc cgcgcccagt gcgcccctgc cgatgaacgc cgtctggcga acaattcgcg
     4561 gatacattct gtggcaatac gagcgaggca ccctccacta cgacatcatg gtcacgctga
     4621 tcctgacctt tgtgcttttc tcgcccaaag taatcaattt caacgacaaa ccgatcgccc
     4681 gcaacccgca tccgacagat gtcatcgtct ccactgacgc ggagggccat ttggtgtacc
     4741 aggtctccgc cagcgccatt tcgccgggcg acgaccgcgc gatccgcgat cagctgctgc
     4801 gcatcattga gccgatttcc ggagcggttt cgatcgtgag ttacgaaaca gtggccgatg
     4861 gcaaaggcaa agtgcagagc tataaggtac aggtgaaacg agaatagtga aggcgcacgc
     4921 cacgatttcc gtcgctctcc tgccgatgct ggccgcgctc actcttctcc cgcagcagtc
     4981 ccagaattct ccgctcgact ccgttcttaa gaaaatggac gagaccgccg ccagctttcg
     5041 caccgcccag gccgacttcg agtgggaccg ctacgagaaa gtcatcgacg aagtcgatga
     5101 cgtccagagc ggcaccattt actaccgccg catcggcaag gacggcaaag aagtcgagat
     5161 gaaagccgat atcaccaagc ccgaccgcaa gtatgtgctc ttcagcggtg gcaagattca
     5221 gatgtatctg ccgaaacccg accagctcac cgtttacgat gccggcagga atcgctcaga
     5281 ttttgagact tatcttgtgc tgggctttgg cggcagcggg catgacctga tgaaggcatt
     5341 cgacgtcact tacatgggcc cggaaacgat taacggagtc gctaccgcga agctgcaact
     5401 cgtccccaag tccgaaaagg tacgcggtac attcaagcag atcttgctct ggatcgatct
     5461 tgcgaacggc atctccgagc agcaacagtt catcgaaccg cagggagact accgcctggc
     5521 gaagtactca tccgtacaag tcaatggcaa gaagattccg gacgacgtct tcaaactgaa
     5581 aaccaccagc aagacccaga ccatctcgcc gcacggttaa gttgttgaat ctaaaaggca
     5641 agaaggatgc tgagatttcg ggagcgaggc gctatactgg ccccacctca gtcgggttca
     5701 ttcatggcca aggtttttac gattactgtc cctcctaagc tgggaaataa gcgcagagag
     5761 cgtacctcgg acccccgtta cgtcgacggg caacgcctgc tggtcgaggc gatgaaatat
     5821 cttgcacagg tcgatcctgt caagaaccg
//
LOCUS       DASPUL010000477         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_231256, whole genome shotgun sequence.
ACCESSION   DASPUL010000477 DASPUL010000000
VERSION     DASPUL010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231256"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..1845)
                     /locus_tag="VEJ00_11355"
     CDS             complement(373..1845)
                     /locus_tag="VEJ00_11355"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HXX01806.1"
                     /translation="MRPKVELLGKPLIERILEEAFQLIEDPGVRVAPYVVDLLRGAGI
                     TVKDGVAHIPESLARRLLDLVPRGFCLYTRSGAPTVRYGGDDVHFDPGSSCLNILDPE
                     TQQARPALSADLVRLVQVAEKLPQFAAQSTAMVCNDVPPEIGDWYRLLLVLWYSEKPV
                     VTGAFSASSLHTLLELLAIESGGQEALRDHPRAIFDVCPSPPLNWSEFASQNLVDLAR
                     AGVPAEIVSMPLAGATAPVTLAGSVVQHAAECISGITIHQLAQPGSPIVWGGAPAIFD
                     MRTGKTPMGAIETAMLDVACAQVGKYLGLPTHAYMVAGDGRLIDAQVEMESGMSAVLG
                     ALAGINMISGAGMLDYLACHSIEKLVIDAEAIASAQRLIEGIEPRGDSLAVAMFAQTG
                     LHGDFLKLKETRALFRKEQHFPSAIIDRGLPSPDGAPGILDRARQRVEELLSQYERHP
                     LPEDREKEIISFAEREGKKAGLEGLPEILKPEYAPDPKLA"
BASE COUNT          388 a          632 c          701 g          431 t
ORIGIN      
        1 ttgagatctc cgggcagtcg acggtgatca cggagtaagg gtgtgttcgg gtctaattca
       61 aaaggtcctt tggttcgggt tgttgccggc ttgggtggcc tggcagttct cgggtggggt
      121 cttcacgcga ttctgtggca gccggatttg cactacacaa actggttcgg ggaactgatc
      181 ttcgcgccga ttgcaattct gtttgggtgg tgatcctgtt tggtgccttg ttcaagcctg
      241 agatcttaac tgcatcttcc aaacggtcca aacggtagag ggccctcagg ggctaaagcc
      301 caccgaatct agaaccgctt acgcggccct gaagggccgc tcttccacgt aacctccttg
      361 gtcttgttca ggtcaggcaa gcttcgggtc gggagcgtat tccggcttga ggatttcggg
      421 cagcccttcc aaccccgcct tctttccttc gcgttctgcg aaggagatga tctctttctc
      481 ccgatcctca ggcagtggat ggcgttcgta ctgcgagagc agctcttcga cgcgctgacg
      541 tgcacggtcg agaatgccgg gcgcgccgtc cgggctgggc aggccgcggt cgatgatcgc
      601 ggagggaaag tgctgctctt tgcgaaagag ggcgcgagtt tctttgagct tgaggaaatc
      661 gccgtgcaag cccgtttgcg cgaacatggc gacggccagc gaatcgccgc gtggctcgat
      721 gccctcgatc agccgctgcg ccgaggcaat cgcctcggcg tcaataacca gcttttcgat
      781 gctgtggcag gcgagataat cgagcattcc cgcgcctgag atcatgttga tgccggccag
      841 cgcgccgagc acagccgaca tgccgctctc catttcgacc tgcgcgtcga tcaggcggcc
      901 gtcgccagcg accatatagg cgtgagtcgg caggccgaga tactttccga cctgggcgca
      961 ggcgacgtcg agcatggcgg tttcgatcgc gcccatgggt gtcttgccgg tacgcatgtc
     1021 gaagatggcg ggcgcgcctc cccagacgat gggagatccg ggctgtgcca gctggtggat
     1081 ggtgatgccg ctgatgcatt cggccgcgtg ctgcacgacg gagccagcga gcgtgacggg
     1141 agcggttgct cccgccagcg gcatgctgac gatttcggcc gggactcctg cacgcgcgag
     1201 gtcaacgaga ttctgcgagg cgaactccga ccagttcagc ggcggcgacg ggcagacgtc
     1261 gaagatagct cgcgggtggt cgcgcaatgc ctcctggccg ccgctctcga tggcaagcaa
     1321 ctcgagaagg gtgtgcaggc ttgaggcaga aaaggctccg gtgacgaccg gcttttccga
     1381 ataccacagc accaacagca ggcgatacca gtcgccgatt tccgggggaa cgtcgttgca
     1441 gaccatggcc gtggactgag cggcgaactg cggcagcttc tcggcgacct gtaccaggcg
     1501 cacgagatca gccgacagcg cgggacgtgc ttgctgggtt tcaggatcga ggatgttcag
     1561 gcacgatgag ccgggatcga aatgaacatc gtctccgcca tagcggacgg tgggggcacc
     1621 gctgcgagtg tagaggcaga agccgcgagg gacgaggtcg agcaagcggc gcgccagaga
     1681 ctcggggatg tgcgcaacac catctttaac cgtgatgccc gcgccgcgca gcagatcgac
     1741 gacgtagggc gcgacacgca caccgggatc ctcgatgagt tgaaacgcct cttcgaggat
     1801 gcgctcgatg agcggcttgc cgaggagttc gactttgggt ctcatgtctc cctgcgtatg
     1861 gtcattccga gcgcagcgag gaatccctac tcccgcaact gtagtctggg ccgtagggat
     1921 tctccgacta gggcgtccgt gcgctttccg cacggccacc ttcgctcaga atgacagcta
     1981 tgcacagcac acatcagatt tgaaccgatt cctgagtcaa tttctgctgc atcagttcct
     2041 gcgccagcgc gacggcgctc atggcgtctt tggcgtagcc gtcggcaccg atttcttccg
     2101 cccaccggcg cgtcacaggc gctccaccga ccatgacctt gacctgggag cg
//
LOCUS       DASPUL010000478         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_87566, whole genome shotgun sequence.
ACCESSION   DASPUL010000478 DASPUL010000000
VERSION     DASPUL010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87566"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..229
                     /locus_tag="VEJ00_11360"
                     /pseudo
     CDS             50..229
                     /locus_tag="VEJ00_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
     gene            369..1190
                     /locus_tag="VEJ00_11365"
     CDS             369..1190
                     /locus_tag="VEJ00_11365"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXX01807.1"
                     /translation="MSVSAVAVGQMQGAPPPEKTAVVYGQNIRYYEAGQGPAVILLHG
                     LGAVKEVWLGNLAALAGKYHVYAIDQLGFGHSDKPLLDYRIGTWVDSLQEFMRSQNIA
                     KATLAGNSLGGWIALDFTVQHPDMVDKLVLVDAAGLTWGGGSVPAVELNPSSVAETRT
                     LLEGIFYDKKMVTDQFVEDVFENHMRNNDGYTIQRTLAGFVHPQFEDDNLKSVHAPTL
                     IVWGRQDQLIGVERAERFHTGIPGSKLVIFDQCGHVPQIEKSAEFNQALLEFLGK"
     gene            complement(1240..1914)
                     /locus_tag="VEJ00_11370"
     CDS             complement(1240..1914)
                     /locus_tag="VEJ00_11370"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HXX01808.1"
                     /translation="MAAIKLVVFDIAGTIIEDHGEVVSAFSVALRKNDIPFPEHELKR
                     WKGASKREVIRHFVEQSGNRNPAELVEATYLCFCAELERLYSGQVTPVAGASETFDWC
                     SKHEIQMASTTGFYSEISDLILKKTGWRDVFAANISSSNVRQGRPAPFMIFRAMEATG
                     VQNVRQVINVGDTPLDLQSGHNAGVRGVVGVLTGEHDRKSLESAPHTHIIGSIAEFPE
                     LIRREF"
     gene            1989..>2709
                     /locus_tag="VEJ00_11375"
     CDS             1989..>2709
                     /locus_tag="VEJ00_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01809.1"
                     /translation="MKKLRVGVLFGGRSGEHEVSLLSAASVLDAIDKEKYEVVPIGIT
                     KEGRWLTAEHAENLLTGKHVEPRHLRAGDPDTTPAAAVLEQGESVVVPPEPVHRESGL
                     MPFQSDAALTRRASDRAINVDVIFPVLHGTFGEDGTIQGLLELADIPYVGAGVLGSAA
                     GMDKDIMKSLFIAAGIPIVRHVTILRSAWEKEPKKVEKLVGSKLKYPVFVKPANLGSS
                     VGISKAHDRKELGPAIEEAARF"
BASE COUNT          548 a          780 c          843 g          538 t
ORIGIN      
        1 cgctcgacat ggattggatc gatcctgaag acgcgccggg cgtggcacgc cggtgcgcgg
       61 cggcgcgacg tatcgcgagg cgcatctggc catggagatc atcgccgatc acggccgcat
      121 gctgagcttc gagatcgtcg aggtgaatcc cgtcatcgac gagcacaacc agactgctga
      181 tctggcggtg gagctggcgc tctcggcgtt cgggaagaag attctgtgac agcagtggcc
      241 agtgatcagt ggccagtttt cggttttgcc gtgcagtctc ccggttgggc cttgaacttc
      301 actttggggt atttttcatg cgaaaagcaa gttgcagcgt tcaccttttt ctcgcaacag
      361 ttttgaccgt gagcgtaagc gcagttgcgg ttggacaaat gcagggtgct ccaccgcccg
      421 aaaaaacggc ggtcgtttac ggccaaaaca ttcgctacta cgaagccggg caggggccgg
      481 cggtgatcct gctgcacggc ctgggcgcgg tcaaggaagt ctggctgggc aatttagccg
      541 cgctcgccgg caaatatcac gtctacgcga ttgatcagct tggcttcggc cattcggaca
      601 agccgctgct cgactaccgg atcgggacct gggtcgattc cctgcaggag ttcatgcggt
      661 cgcagaacat cgccaaggcg acgctggccg gtaattcgct gggcggatgg atcgcgctcg
      721 acttcaccgt gcaacacccg gacatggtcg acaagctggt gctggtggac gcagcggggc
      781 tcacctgggg aggaggatcg gtgccggccg tcgaactgaa cccgtcgtcg gtcgccgaga
      841 cgcgaacttt gctggaaggc atcttctacg acaagaagat ggtgaccgat caattcgtgg
      901 aagacgtctt tgagaaccac atgcgcaaca atgacggata cacgatccag cgcaccctgg
      961 cgggatttgt gcatcctcag ttcgaagacg acaacctcaa gtccgttcat gcgcccacgc
     1021 tcatcgtctg ggggcgccag gaccagctga tcggcgtgga gcgcgccgag cggtttcaca
     1081 ccggcattcc cgggtcgaag ctggtcatct tcgaccaatg cggtcacgtt ccgcagatcg
     1141 agaagtcagc agagttcaat caggcgctgc tggagtttct gggcaagtga tggcgaccgg
     1201 cggccggggg cgctactcat ggaaagtaaa accatgcgct caaaactcgc gtcgaatcaa
     1261 ttccggaaat tcggcgatgc tgccaatgat gtgagtgtgc ggggctgatt ccaaactctt
     1321 ccggtcgtgt tctcccgtga gtacaccgac cacgccccgc acgccggcat tgtgtcctga
     1381 ctgaagatca agtggagtat ctccaacatt gatcacctgc cggacgtttt gcacgcccgt
     1441 cgcctccatg gcgcgaaaga tcatgaatgg cgccggacgt ccctggcgca cattgctgct
     1501 ggagatattg gccgcgaata catcgcgcca ccccgtcttc ttcagaatca ggtcgctgat
     1561 ttcgctgtaa aagccggtag tgctcgccat ctggatctca tgcttgctgc accaatcgaa
     1621 ggtctccgac gcacctgcaa ccggcgtcac ttgtccggag tagaggcgct ccagttcggc
     1681 acaaaagcaa agataggtcg cctctaccag ctccgccgga ttcctgttcc cgctttgttc
     1741 aacgaagtgg cggatgacct cgcgcttgga agctcctttc cagcgcttca attcgtgctc
     1801 cgggaatgga atgtcatttt tcctcagtgc aacggagaaa gcgctgacta cttccccgtg
     1861 atcctcgatg atggtacccg caatatcaaa gacgaccagc ttgatcgcag ccatagccgc
     1921 agattttacc agcaggaatc ttgcctgcga ttgacgcggg acttcttgcg cgtccacact
     1981 gaaaggtcat gaaaaagctt cgcgtgggag ttctcttcgg cgggcgcagc ggggagcatg
     2041 aagtctcgct gctctcggcg gcgtcggtgc tcgacgccat cgacaaggaa aagtatgaag
     2101 tcgtgcccat cggcatcacc aaggagggcc gctggctgac ggcggagcac gccgagaatc
     2161 tgctcacggg caaacatgtc gagccgcgcc acctgcgcgc cggcgatccc gatacaactc
     2221 cggcagcggc tgtgctggag cagggcgagt cggtggtcgt gccgcccgaa ccggtgcacc
     2281 gcgagagcgg gctgatgccc ttccagagcg atgccgccct gacgcggcgc gccagtgacc
     2341 gcgccatcaa cgtggacgtc attttccccg tgctgcacgg cacgtttggc gaggacggca
     2401 ccattcaggg cctgcttgaa ctcgccgaca tcccttatgt gggggccggc gtgctgggct
     2461 cggcagcggg catggacaaa gacatcatga agtcgctgtt catcgccgcg ggaattccaa
     2521 ttgtcagaca tgtaacgatt ttgcgcagcg cgtgggagaa ggagccgaag aaagtcgaga
     2581 agctggttgg cagcaagctg aagtatccgg tgttcgtgaa accggcgaac ctgggatcgt
     2641 cggtcgggat cagcaaggcg catgatcgca aggagctcgg acccgcgatt gaggaagcgg
     2701 cgaggttcg
//
LOCUS       DASPUL010000479         9659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_498567, whole genome shotgun sequence.
ACCESSION   DASPUL010000479 DASPUL010000000
VERSION     DASPUL010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9659)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9659
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498567"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2007)
                     /locus_tag="VEJ00_11380"
     CDS             complement(<1..2007)
                     /locus_tag="VEJ00_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXX01810.1"
                     /translation="MIGQSISHYRILEKLGGGGMGVVYKAEDTRLHRFVALKFLPDEV
                     ARDPQALARFQREAQAASALNHPNICTIHDIGEENGLAFIAMEYLEGVTLKHRIGGRA
                     LDLDFFLPLAIEIADALDAAHSKGIIHRDIKPANLFITERGHAKILDFGLAKVAPAAI
                     SSSQIAAFNTQTQSLDEAHLTSPGTMVGTVAYMSPEQIRGKELDARTDLFSFGAVLYE
                     MATGALPFRGETSAMICEAIVNRAPVAPVRLNPDLPAELERIINKALEKDRDLRYRSA
                     ADLQTDLKRLKRDTESGRAPVLETARPAQRPSWKSPRWSRWVVVGILAIFAVLEVLLR
                     APLPPPKITGSKQITNDGLFKFNLVTDGNRIYFNENAPSRRAIAQASIAGGETAPIDV
                     PFDNATVNDVSQERSELLVTQELLETVDNPYWSVPLPAGSPRRLGDLSAHDAVWAPDG
                     KLLFGKGRDLYIADHDGANPRKIASSHDIPGSPSFSPDGSRIRFTTYDLINNVNAIWE
                     VRADGSGLHPVFPGWNNPPNECCGKWTPDSKYYLFQSTRDGVAQVWIVPDRSDWWRKI
                     SHDPVQLTTGPLQFGVPLPSQDGKKLFVVGVQQRAELVRYDAQSGDFAPYLEGISAGD
                     VDFSRDGQWVTYVSYPDDTLWRSKLDGSSRLQLTYAPMRTGVPHW"
     gene            complement(2191..2664)
                     /locus_tag="VEJ00_11385"
     CDS             complement(2191..2664)
                     /locus_tag="VEJ00_11385"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXX01811.1"
                     /translation="MSTPSAPAMTAPAETAPRPVTTTTADQATIGKSLVIKGEVTGSE
                     SLYIDGRVEGSINLSGNRVTIGRNGVVAANINAREIVVLGKVRGNLTASDRVDIRSDG
                     SLTGDVIAARISIEDGAFFKGGIDIRKGGQPQQTKPNGEEKAMTVGEPAAIGARA"
     gene            3091..3687
                     /locus_tag="VEJ00_11390"
     CDS             3091..3687
                     /locus_tag="VEJ00_11390"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX01812.1"
                     /translation="MPESAVVTPAALIVARPEPDEYTPYYHCYISLVAGSDILATLDS
                     QRRDTVLLLSGRDEEDGDFRYAPDKWNAKEVLGHLCDTERIFAYRALRIARGDRTPIE
                     GFEQDDYVRNSPFASRPLAEVIEDYIAVRRATITLLRNLDEAAWMRRGVANRNEISVR
                     AIAYLIAGHELHHRGILQGKYFSSSLGRRGCSDLGIGS"
     gene            3753..4256
                     /locus_tag="VEJ00_11395"
     CDS             3753..4256
                     /locus_tag="VEJ00_11395"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX01813.1"
                     /translation="MSRRALTELLRARGAHADPIACVEDITAEVAVRQVAGFPHSIGQ
                     LVFHMNYWMNYDLRRARGERPPYPQHNAESFPAAPSPANAEEWDRLRRDFAWFLGEHA
                     RLAESSRAELERAVEATTERHNELAGTLEAILWQTVAHNSYHVGQIAMIRRTLGAWPP
                     KGGGDSW"
     gene            4418..7051
                     /locus_tag="VEJ00_11400"
     CDS             4418..7051
                     /locus_tag="VEJ00_11400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HXX01814.1"
                     /translation="MLSDSAILKHIARQPKRTAGFKQLVRELGLHGDERRELNERLQK
                     LVAGGGLVTLDSDRYALPQAADKNLVAGRLSMHRDGFGFVIPDPQSLNASLRARLTGD
                     IFIAPHLIGNAMHGDLVLVDITNVRPDGRAEGRIVQPVRRAHPTVVGIFHYGGRRNYV
                     TPMDAKITQEIVIPAGMEWPEDDSSSPTTIGAAREIPRPAASPPPTKVGGLPESAGLR
                     NDAKKSKGTSKRSVDRVLGQEAARRSEWDDLEGVVVDVEITDWPSATQNPRGRVIEIL
                     GREDDFGVDVEITIRKFHLPHHFPPAALEEAQNAPTVISARELHRRRDFRDLPIVTID
                     GETARDFDDAVLVRKLENANFELQVHIADVAHYVTPGSALDQEARLRGTSVYFPDRAV
                     PMLPLELSTDICSLRPQVDRLVMSCVMEMDHSGEIVGYELSEGVIRSAERMTYTAVNA
                     VLEGDAAMRQRYAPLVGNFELMRDLALILNRKRERRGSIDFDLPEPVIEFDEFGLMKS
                     IARSERNVAHRLIEEFMLSANECVAQHLESQRIASLYRIHEKPDAKRVYDFETIAATF
                     GYSLGVGALPIQRVQMKADRRAAYGTGKRVREIDVPKEVHITPRMYQKLTEKIAGKPE
                     ERILSYLMLRSLKQARYSEENLGHFALAAASYTHFTSPIRRYPDLIVHRILKEALREE
                     LTSAAEAASSIERLAARLEAAPFQSKPSQGKPAPWSKQRVASDRTRAGVPAPHESLGG
                     PIALEELHEIAEESSQAERRADDAERELMEWKKVKFMQDRVGEDFDGLIISVTKFGFF
                     VELTDLFVEGLVSLNTLTDDRYTYHENTREIIGQRSRKTYRLGQRIRVLVDRIDPVEK
                     KIQFAVVEEEPVRSEKRKSRK"
     gene            complement(7068..7460)
                     /locus_tag="VEJ00_11405"
     CDS             complement(7068..7460)
                     /locus_tag="VEJ00_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01815.1"
                     /translation="MFLRFVTGEVHEESHQALGVFQAAYRLRAAGLLSDEEEDHLEEI
                     REWFNENLEKPSRFTSAKPPYYRKGQNGISWSKDSAHEHIRRIREMVAFLKRHDVSVR
                     MIKSSRPGYIVYEDAHQVVAVPFADSKL"
     gene            complement(7604..8983)
                     /gene="glmM"
                     /locus_tag="VEJ00_11410"
     CDS             complement(7604..8983)
                     /gene="glmM"
                     /locus_tag="VEJ00_11410"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HXX01816.1"
                     /translation="MTQQARQLFGTDGIRGVAGEFPLTKQSTHLIGRALGHDLMRATP
                     KAKAVIGQDTRQSSRWIADRISEGLAAVGVDVHSAGVITTPGVAYLARSRDFAAGVVI
                     SASHNPWTDNGIKVFSGDGFKLTDTRELAIETEIFALLQNPSAADDTALRIPAPSLPG
                     EAELRHAYVKSLAGSVSSDLRKLRVLVDCANGAATAEAPELFRSLGVQATFMHISPDG
                     KNINERCGALHPETLGETLAAAPGKFDLGVTFDGDADRALFCDAAGRVVNGDGVLLAA
                     ARDLQSQGKLKADTVVATTMSNMGLEIALKNSGIRMLRANVGDKYVLEEMLKTGATLG
                     GEQSGHIIFRDGDSTTGDGLLTALRLMDIIVRSGRSLAELVPDLKVFPQKIQNVRVRE
                     KIPFAQVLAVQAAIEAAERELNGNGRVVVRYSGTEALARVMVEAESEEKMKALVATIA
                     AEIQKALGV"
     gene            complement(9119..>9659)
                     /locus_tag="VEJ00_11415"
     CDS             complement(9119..>9659)
                     /locus_tag="VEJ00_11415"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HXX01817.1"
                     /translation="FQHVPPHLEISSEAIPEAQIRVRGPERVIRRLQASEVHAEIDLS
                     NARPEEHTYDLTSQQVRHPREVTVVQVVPSQLHLAFDTRLTRDVDIHPRITGSFSDGE
                     QIVKVVSDPARITITGPRHHVETTDAATTDPVDASGTMGSAVFTTNVYVADPLVQVVQ
                     PTSVRVTVIVEKVGNTSAH"
BASE COUNT         1869 a         2636 c         3263 g         1891 t
ORIGIN      
        1 ccagtgaggc acccccgtcc gcatgggcgc gtaggtcagt tgcagacggg agctgccgtc
       61 gagcttgctt cgccaaagcg tgtcgtcggg atagctgaca taggtcaccc actgcccgtc
      121 gcgcgaaaaa tccacatcgc cggcggagat gccttcgaga tagggcgcga aatcgccgga
      181 ttgggcgtcg tagcgaacca gctcggcgcg ctgctgcaca ccgactacga agagcttttt
      241 gccgtcttga ctgggaagcg gaacgccaaa ttgcaagggg ccagtggtga gctgaaccgg
      301 atcgtgcgag attttccgcc accagtcgga gcggtcgggc acgatccaga cctgggccac
      361 gccgtcgcgg gtgctctgaa agagatagta cttgctgtcc ggggtccact tgccgcagca
      421 ctcgttggga ggattgttcc agccagggaa aacgggatgc aggccacttc catcggcgcg
      481 gacctcccaa atggcattca cgttgttgat cagatcgtac gtggtgaatc ggatacggga
      541 tccgtcgggt gaaaagctcg ggctccctgg aatatcgtgc gaggaggcaa tttttcgagg
      601 attcgcgccg tcgtggtctg cgatatagag atcacggccc tttccaaaca gcaattttcc
      661 atccggcgcc cacacagcgt cgtgtgcgga aagatccccg aggcggcggg gcgaaccggc
      721 gggaagaggg acagaccaat aggggttatc caccgtctcc agcagttcct gtgtaaccag
      781 cagttccgat cgctcctgcg agacgtcatt cacggtggca ttatcaaacg gcacatcgat
      841 gggtgcggtc tcgcctccgg cgatggacgc ctgggcgatg gcgcgacgac tgggagcatt
      901 ctcgttgaaa taaatgcgat tgccgtcggt gacgagattg aacttgaaca ggccgtcgtt
      961 ggtgatttgc tttgatccgg tgatttttgg cgggggaagc ggcgcgcgca acaagacctc
     1021 gagaactgcg aagatggcaa ggatgccgac cacgacccat ctcgaccaac gtggcgactt
     1081 ccacgaaggc cgttgtgcag gtcttgcagt ctcgagcacg ggcgctcttc ccgactcggt
     1141 gtcgcgtttg aggcgcttga ggtcggtttg caggtcggcg gcggagcggt aacgcaggtc
     1201 gcgatctttc tcgagagcct tgttgatgat tcgttccagc tcggcgggca gatcaggatt
     1261 caagcgcaca ggcgcgaccg gggcgcggtt gacgatggct tcgcagatca tggccgacgt
     1321 ctcgccccgg aagggtaagg ctccggtcgc catctcgtac aagactgcgc cgaaggaaaa
     1381 gagatcggtg cgcgcatcga gttctttgcc gcgaatctgc tcgggcgaca tgtaggcgac
     1441 ggtcccgacc atggtgccgg ggctggtgag atgggcctca tctagggact gggtctgagt
     1501 gttgaaagct gcgatctggc tggacgaaat ggcagcgggc gcgactttgg ccaggccgaa
     1561 gtcgagaatc ttggcgtgcc cgcgctcggt gatgaagagg ttggcgggct tgatgtcgcg
     1621 atggatgatg cctttgctgt gggcggcatc gagggcgtcg gcgatttcga tggcgagcgg
     1681 gaggaaaaaa tcgagatcga gggctcggcc gccgatgcgg tgcttcaggg tgacgccttc
     1741 gaggtattcc atggcgatga aggccaggcc gttctcctcg ccaatgtcat ggatggtgca
     1801 gatgttggga tgattcaggg cggaagcggc ttgcgcttcg cgctggaatc gggccagagc
     1861 ctgcgggtcg cgcgcaactt cgtcgggcag gaatttgagg gcgacgaagc ggtgcaggcg
     1921 agtgtcttcg gctttgtaga ccacacccat cccgccgcca ccgagctttt cgaggatgcg
     1981 gtagtgcgag atggactggc cgatcatgtt cgaatcgcgg ccaaatgtta cgcgcgggaa
     2041 ttggccaggt caacgagagt ggaccgggag agcacaaaaa aaacgggcat cctgatggat
     2101 gcccggtgcg atgcggaaag ctaacacccc cacttctcgc gcaaaaagca cgcgcgagaa
     2161 atggggcacc cgaaagttcc cggggctgat ctacgcgcgg gcgccgatgg cggccggctc
     2221 tccgaccgtc atggccttct cttcgccatt gggcttggtc tgctgcggct gcccgccctt
     2281 gcggatgtcg atgccgccct tgaaaaaggc gccgtcctcg atggagatgc gggcggcgat
     2341 gacgtcgccg gtgagcgagc cgtcactgcg aatgtcgacg cggtcgctgg cggtcaggtt
     2401 cccgcgcacc ttgcccagca cgacgatttc gcgggcgtta atgttcgcgg cgacgacgcc
     2461 gttgcggccg atagtcactc ggttgccgga aaggttgatg gagccctcga cgcggccgtc
     2521 gatgtacaag gactcggagc cggtcacctc gcccttgatc accagcgact tgccgatggt
     2581 ggcctggtca gcggtcgtgg tcgtgaccgg gcgcggagcg gtttcagcgg gggctgtcat
     2641 cgcaggggcg ctgggggtgc tcatggtcgg acgctcccct tcggcaggac gggtcggggt
     2701 ctgaggctga ttggtcggct tccacatggg tgaaatggtt tcctttcctg ttggaattag
     2761 gggtcacagg ccgcacgttc gtgaccaaag cgaattgtac tcccgcgtct caaaatgagc
     2821 gctgtggaaa accctttatt ttcaatggga gttcctcgga tggtacgtcc tgaagtggac
     2881 ttcgaagtct gaaacgatgg gctgcctcca tctccttttt gcctagtccg cgtcgtgtct
     2941 taggaaaaaa tcaccgggcc gtacccttcc acagggcccg ggcgttatcc ttccgggatc
     3001 gaagatttat cgaaaacgga ataggaacca ggacgtctaa gttgtaagga aactggggac
     3061 ccacgaattc aatcgaaggt ggaatcgagc atgccggaat cagccgttgt tacccctgca
     3121 gcattgatcg tcgctcgccc ggagccggac gagtatacgc cttattatca ttgctacatt
     3181 tctcttgtag ctggatcgga catcctggcg acgctggact cgcagcgccg cgatacggtg
     3241 ctcttgctct cagggcggga cgaggaagac ggggacttcc gctacgcacc cgacaagtgg
     3301 aacgccaaag aagtgctcgg gcacttatgc gacacggagc ggattttcgc gtatcgcgcg
     3361 ctgcgtattg cacgcggcga ccggacgccc attgaaggct tcgaacaaga cgactatgtg
     3421 cggaatagcc cgtttgccag ccgaccgctg gcggaggtga tcgaggacta cattgcggtg
     3481 cggcgggcga cgatcacgct ccttcgcaat ctcgacgaag cggcctggat gcgccgcggt
     3541 gtggccaaca ggaatgagat cagcgtgcgg gcgattgctt atctgatcgc ggggcatgag
     3601 ctgcatcacc gcgggatttt gcaggggaag tacttctcat ccagccttgg gcggcggggg
     3661 tgttcggacc tgggaatcgg gtcttgacag attggtcgct gggcggcggc ttccacgtct
     3721 gctataacct aggctcctga ggccgatctt ccatgtctcg gcgcgcactc accgaactgc
     3781 ttcgagcgcg aggcgcgcac gccgatccga tcgcgtgcgt cgaggatatc actgccgaag
     3841 tggcggtgcg gcaggtcgcg ggatttccgc actcgatcgg gcagctcgtc ttccacatga
     3901 attactggat gaactacgat ctgcggcgcg cgcggggcga gcggccgccg tatccccagc
     3961 acaatgcgga gagctttccg gcggcgccgt cgcctgcgaa tgccgaggag tgggaccggc
     4021 tgcgcaggga cttcgcgtgg tttctgggtg agcatgcccg gctggcggaa tcgtcgcggg
     4081 cggagctgga gcgcgcggtg gaagctacga ccgagcggca caacgaactc gcgggaactt
     4141 tagaggccat tttgtggcag acggtggcgc acaacagcta ccacgtgggg cagattgcaa
     4201 tgatccggcg gacgctgggg gcgtggccgc cgaagggcgg cggggattcg tggtaggtgt
     4261 tttaccacag gggctgaagc cccaaccagt tcctggcctt ttacgcggcg ctgaagcgcc
     4321 gttcttccac ggtgacgaaa tcgccgggtc tttcgcggca ttccaacgcc gagctgtggt
     4381 ttatgctgaa agctgacggc tgatagctgt cagccccatg ctctccgatt ccgccatcct
     4441 caaacacatc gcgcggcagc cgaagcgcac ggctggcttc aagcagctgg tgcgcgaact
     4501 tggactgcac ggggacgagc gccgcgagct gaacgagcgc ctgcagaagc tggtcgcagg
     4561 cggagggctg gttacgcttg attccgatcg ctacgcactt cctcaagctg ctgacaagaa
     4621 tctggtcgcc gggcggctca gcatgcaccg cgacgggttc gggttcgtca ttcctgatcc
     4681 gcagtcgctg aatgcgtcgc tccgggcgcg tctgactggc gacatcttca ttgcgccaca
     4741 tctgatcggc aacgccatgc atggggacct cgtgctggtg gatatcacca acgtgcgtcc
     4801 ggacgggcgg gcggaagggc gcattgtgca gccggtgcgg cgggcgcatc cgacggtggt
     4861 ggggattttt cactacggcg ggcggaggaa ctatgtcacg ccgatggatg caaagatcac
     4921 gcaggagatc gtgattccgg cgggaatgga gtggccggag gatgattcgt ccagcccgac
     4981 gacaattggc gctgcacgcg agattcctcg ccccgcggct tcgccgcccc cgactaaagt
     5041 cgggggtcta ccagaaagcg cggggcttcg gaatgacgcc aagaaaagca agggaacgag
     5101 taagagatct gtcgatcgtg ttctgggtca agaggcggcg cggcgctcgg agtgggacga
     5161 tctggagggc gtagtcgttg atgtggagat caccgactgg ccttctgcga cgcagaaccc
     5221 gcgggggcgg gtgattgaaa ttctcgggcg cgaggatgat tttggcgtcg acgtcgagat
     5281 cacgatccgc aagttccact tgccgcatca ttttccgccg gcagcgctgg aagaggcaca
     5341 gaatgcgccg accgtgattt cggcgcggga attgcacagg cggcgcgact ttcgtgatct
     5401 gccaattgtg acgatcgatg gcgaaacagc gcgcgacttc gacgatgcgg tgctggtgcg
     5461 caaactggag aacgccaact ttgaattgca ggtacacatt gccgacgtgg cgcattacgt
     5521 gacgccgggt tcagccctcg atcaggaggc gcggctgcgg gggacgagcg tgtatttccc
     5581 tgaccgcgcc gtgcccatgc taccgctcga actttccacc gacatctgca gcctgcggcc
     5641 gcaggtggac aggctggtga tgtcgtgcgt gatggagatg gaccatagcg gcgaaattgt
     5701 ggggtacgag ttgagcgagg gcgtgatccg gtcggccgag cgcatgactt acacggcggt
     5761 gaacgcggtg ctcgagggcg atgcagcgat gcgccagcgt tatgcgccgt tggtcgggaa
     5821 ttttgagctg atgcgcgacc tggcgctgat tctgaatcgc aagcgcgagc ggcggggatc
     5881 gattgacttc gacctgccgg agccggtgat cgaatttgac gagttcgggc tgatgaagag
     5941 cattgcgcgc tcggagcgga acgtcgcgca ccggctgatc gaggagttca tgctttcggc
     6001 gaacgagtgc gtggcgcagc atctggaatc gcagcggatt gcgtcgctgt atcgcattca
     6061 cgagaagccg gatgcgaagc gcgtgtacga ctttgaaacg attgcggcga cgtttggata
     6121 ttcgctgggc gtgggagcgt tgccgattca gcgtgtgcag atgaaggccg accggcgcgc
     6181 ggcttacgga acaggcaagc gcgtgcgtga gattgatgtg ccgaaagagg tgcacatcac
     6241 gccgcgcatg tatcagaagc tgacggagaa gattgcgggc aagccggagg agcggattct
     6301 gagctatctg atgctgcggt cgctcaagca ggcgcggtat tcggaggaga acctggggca
     6361 ctttgcgctg gcggcggctt cgtatacgca ctttacgtcg ccgattcgaa ggtatccgga
     6421 tctgatagtg cacaggattt tgaaggaggc gctgagagaa gagctcacct cagcggctga
     6481 agctgcttct tccattgaaa gactggccgc gcggctggaa gctgcgccct ttcaaagcaa
     6541 gccctctcaa ggtaagccgg cgccatggtc gaagcagcgg gtggcctccg acaggacgcg
     6601 ggcaggagtg cccgcgccac acgagtcttt gggtgggccg attgctctgg aggaattgca
     6661 cgagatcgcg gaggagtcga gccaggccga gcggcgggcc gacgatgccg agcgcgagct
     6721 gatggaatgg aagaaagtga agttcatgca ggatcgggtg ggcgaggact tcgacggact
     6781 gatcatcagc gtgacgaagt tcggattctt tgtcgagctc accgatcttt tcgttgaggg
     6841 attggtatcg ctcaatacgc tgactgatga caggtatacg tatcacgaaa atacgcggga
     6901 gattattgga cagcggtcgc ggaagactta ccgattaggg cagaggattc gggtgctggt
     6961 cgaccggatc gatccggtgg agaagaagat tcagtttgcg gtggtggagg aagagccggt
     7021 gcggtcggag aaacggaaga gccgaaagtg aatgcgtgag ggaagcatca gagttttgag
     7081 tctgcaaacg ggacggcgac gacttgatgt gcgtcttcgt acacaatgta tccgggtcga
     7141 gacgatttga tcattcgcac ggacacatcg tgtcgcttga ggaaggcaac catctctctt
     7201 atcctgcgaa tatgttcgtg cgctgaatct ttagaccagg agattccatt ctgccctttc
     7261 cggtagtaag gaggcttcgc ggaagtaaac ctgctcggct tttccaaatt ctcgttaaac
     7321 cattcgcgaa tctcctcaag atgatcttcc tcttcgtcgg acagcaggcc agcggcgcgt
     7381 aaacggtaag ctgcctggaa aactccaagc gcttgatggg actcctcgtg gacttcgccg
     7441 gtcacgaatc gcaggaacat gtggagtgag cctcccacag gcgaagcgaa ccaggtcacc
     7501 ccgcaggggg ctgaagcccg aatgctctaa gcgtgcctac gcggccctga agggccgctc
     7561 ttccacggcg attccggaca agcattcgca aaagaaatgg agcctagaca cccagtgctt
     7621 tttggatctc tgcggcaatc gtcgcgacaa gggctttcat cttttcttcc gattcggctt
     7681 caaccatgac ccgggccaag gcttcagtgc cggaataacg gacgacaacc cgtccattgc
     7741 cgttgagttc gcgctcagcg gcttcgatcg cggcctgcac ggcgagaacc tgagcaaacg
     7801 gaatcttttc gcgaacccgc acgttctgga tcttctgcgg gaacaccttt agatcgggga
     7861 caagttcagc cagcgacctg ccgctgcgca cgatgatatc catgaggcgg agggcggtca
     7921 gcaggccgtc gccggtggtg gaatcgccgt cgcgaaagat gatgtgaccg gattgctcgc
     7981 cgccgagcgt ggcgccggtc ttcagcatct cttccagaac atacttgtcg ccgacgttag
     8041 cgcgcaacat gcgaatgccg gagttcttca aggcaatctc gagacccata ttcgacatgg
     8101 tagtggcgac gactgtgtcg gctttcaact ttccttgcga ctgcagatcg cgggcggcgg
     8161 caagcagcac accgtcgccg ttgaccacgc gaccggcggc atcacagaac agggcgcggt
     8221 cggcgtcgcc gtcgaaggtc acgcccagat caaactttcc gggcgcagct gcgagcgtct
     8281 cgcccaaggt ttcaggatgg agcgcgccgc agcgttcgtt gatgttcttt ccgtcgggcg
     8341 agatgtgcat aaacgttgct tgaactccca ggctgcgaaa cagttccgga gcttcggcgg
     8401 tcgcggcgcc gttcgcgcaa tcgaccagca cgcgcagctt gcgcagatcg gacgacacgc
     8461 tcccggccag cgatttcaca taggcgtggc gcaactcggc ctcaccgggc agagaagggg
     8521 cgggaatacg gagcgcagtg tcgtcggcgg ccgatggatt ctggagcagc gcgaagattt
     8581 cggtttcaat cgccagttcg cgcgtatcgg tgagcttgaa gccgtcgccc gaaaaaacct
     8641 tgatgccgtt atcagtccag ggattgtgcg aggcggagat gacgacgccg gcagcaaaat
     8701 cccgcgaccg cgcgaggtag gccactcccg gggtcgtgat tacgccggcg ctgtgtacgt
     8761 cgacgccaac ggcggcaagg ccctcggaga tgcggtcggc gatccagcgg ctggactggc
     8821 gcgtgtcctg cccgatcaca gcttttgcct tgggagttgc gcgcatgaga tcgtggccca
     8881 gggcgcggcc gatcaggtgc gtgctctgct tggtcagggg gaattcgccg gccacgccgc
     8941 gaatgccgtc ggtaccgaac agttgccggg cttgctgggt catcagaata attccggaag
     9001 gtgcatgaag aagagcaaca gagatgatat atgagaacgg gcccgagaga gaaaaagcag
     9061 atttctccct tcgctgcggt cgggtcggaa tggcaaacta agtggcgggt taggcgtttc
     9121 aatgcgccga cgtgttgccc accttctcga cgatcacggt gacgcggacc gaagtcggtt
     9181 gcacaacttg caccagcggg tcggcgacgt agacgttcgt ggtgaagacg gcgcttccca
     9241 tggtgccgct cgcgtcgacc ggatcggtag tggcggcatc ggttgtttcg acgtggtgac
     9301 gcggcccggt gatggtgatg cgtgcgggat cggaaacaac cttgacgatc tgctcgccgt
     9361 cgctgaagct tccggtgatc cgcgggtgaa tgtcgacgtc gcgagtgagg cgggtgtcga
     9421 acgcaaggtg gagctgactg ggcacgacct gcaccaccgt cacctcgcgg ggatgacgta
     9481 cctgctgcga agtgaggtca taggtgtgtt cttcaggcct ggcattggag aggtcaatct
     9541 ccgcgtgcac ctcgctggcc tggagccggc gtatgacgcg ctcggggccg cgcacgcgga
     9601 tctgcgcctc gggaatcgcc tccgaactga tttcgagatg cggcggaacg tgctgaaag
//
LOCUS       DASPUL010000480         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_640516, whole genome shotgun sequence.
ACCESSION   DASPUL010000480 DASPUL010000000
VERSION     DASPUL010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640516"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VEJ00_11420"
     CDS             complement(<1..579)
                     /locus_tag="VEJ00_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXX01818.1"
                     /translation="MNRTVRIELAIALERGEFRLEYQPKVSQQAGRVIGLEALVRWQH
                     PERGTLLPRDFIQAAEASNLIVPLGDWVLRTACAQNKAWQDQGLVPVQVSVNVSARQL
                     CQSNFAQQVRGVLSETGLEPKYLQFELVDYTPIEDTQSVIEVLRELRTIGVEFATDNF
                     GVGHSSLSRLRLPLQVLKINRYFIEGLPNDEGR"
     gene            complement(1750..2328)
                     /locus_tag="VEJ00_11425"
     CDS             complement(1750..2328)
                     /locus_tag="VEJ00_11425"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HXX01819.1"
                     /translation="MMIRLTVGTLPLLLLWPLVATAQDAGALLAASRETAGLLIHQLG
                     AQLRAELANGGPEGAVAVCKDIAPELAGRLSRETGWRVSRVSLKTRNPLLGSPDAWEQ
                     RGLEEFDRRVAAGEKAETLEVDEVVEEPAGRYYRYMKALPVQPLCLTCHGTAETIPPD
                     VQQRLRVEYPNDRATGYRAGQVRGAVTVKRPL"
BASE COUNT          491 a          805 c          827 g          498 t
ORIGIN      
        1 ccttccttcg tcgttcggca aaccttcaat gaagtagcga ttgatcttca gtacctgaag
       61 cggaagcctt agacgactga gggacgaatg cccaacgcca aaattgtcag tcgcgaactc
      121 cacgccaatt gtccgcaact cgcgcaggac ctcaatgact gattgagtgt cttcgatggg
      181 ggtgtaatcg accaactcga actgcagata cttgggttca aggccggtct ccgacagaac
      241 ccctcgcact tgctgggcga aattgctctg acacaactgg cgcgccgaaa catttactga
      301 gacttgcacc gggaccaaac cctgatcctg ccatgctttg ttttgcgcgc aggcggtacg
      361 aaggacccag tctccgagcg gcacgatcag gttcgacgcc tctgcggcct ggataaagtc
      421 gcgcggcaac aatgttcccc gctccgggtg ctgccagcga accagcgcct ccagcccaat
      481 cacgcgcccc gcttgctgac tgaccttggg ctggtattcg aggcggaact cgcctctttc
      541 gagcgcgatt gctagctcga ttcgcacggt cctgttcacg atgcactcca gctggctgat
      601 ttgattgcgc cgattaacgg caatcgcgac gcaagctgaa cggcaaccgt tcgcgactac
      661 cgttgtgctg caggggcgcg tcacagcgtc ggctgtgaca tccgagcacg ctcgagcgag
      721 gcgctcttgt gacgaatgtg ctcgccgggc gcggccggtc gtggacgatc cctacgacag
      781 cgagtggcgc cgatacgaat cgctaggggt ctttgcaaat cgagtcagca attgaggcaa
      841 gtacaggcca ccgcgtcgtg gtgatctctt tcggggtggc cctgatcccg tgcactcgcg
      901 gccaatttca acaacacgaa tccggtcgcc tggcgataaa ccctctagtg ctgccatgaa
      961 cggtgcatca aagatggcgt gcgtgtcatg aagggtgatt tgcagtgaag cgttgttcgc
     1021 catttgcgaa gagccgagca gtcgggaagg tgataggcag ggcccaaggc aaaggctgcg
     1081 aaacggcaaa gaagctacgg tgcgcgtcac tagatgtagt gggtcggagc gagtcactac
     1141 acattagctg gagtcgcggg gtcagtggtg cgataattcg cacgggcggc cgccagacca
     1201 agatcgggtg cgccgggagg aggcccataa acctttagga gactcctctc atgcctcgat
     1261 tcaaactaaa atcgatctgc gcaactggtg gcacgttgtt agtcttgggc ccgctggttg
     1321 ccgatcattc tcttttcgat tgcgagccgc tgccagccac ttgctccgca accatgccgc
     1381 gtgagcagct gcacccggtt gatgatggcc cgccatcccc gccgacccac tcgggtggct
     1441 ccggctcgat gagcaccacc agcgcctcgt cgggaacccc tctatacacc gggagcggga
     1501 cattggtcgc gggcccaggc acgatctctg gcagtgggac ggtctggtca acgggtattg
     1561 ctgagggcac aagcagcgct actggggttg gactcggcac cgttgctctt accggccgtg
     1621 gggagggttt tgtaacggga gcgctcgact tcggtgaaag cccggataga gtgcatggtg
     1681 agttcgccgt tggcgtcaag ccgtcggaag aactgaccgg ccgaactacc tagcgacgat
     1741 tgcgttgtct catagaggac gcttcacggt gacagcgccg cgcacctggc ccgcgcgata
     1801 gccggtcgcg cggtcgttcg gatactccac gcgcaggcgc tgctgtacgt cgggaggtat
     1861 cgtttccgcc gtgccgtggc aggtaagaca tagcggctgc acgggcagcg ccttcatata
     1921 gcggtagtat cggccggcgg gttcctccac gacttcgtcc acttccaggg tctcagcttt
     1981 ctcgccggcg gcgacgcgcc ggtcgaactc ctcgagtccg cgttgctccc aggcgtcggg
     2041 cgagccgagc agcgggttgc gggttttgag gctcacccgc gatacgcgcc atccggtctc
     2101 gcgggacaat cggccggcca gctccggtgc tatgtccttg caaacagcca ccgcgccttc
     2161 cggcccgccg ttcgcaagct cggcccgcaa ctgcgcgccg agctggtgga tcagcaggcc
     2221 ggccgtctcg cggctcgcag ccaacagcgc gccggcgtct tgcgccgtgg cgaccaaggg
     2281 ccaaagcagg aggagcggga gagtcccgac ggtcagccga atcatcatga tgcgctccga
     2341 gtgaagtggg cttggcgtag tctaaccggc gcggcctgac ctggctgcgc ccggcgtcgc
     2401 ccacgctggt cgaagggtca ttgaaacgtt ccgccaggtc cccaatgtag agcaggccca
     2461 acgttatggg ccggagaggt ccctatgcgc ttcgacaagt tcacgaccaa gttccagcag
     2521 gccttcgccg atgcgcagag ccgggcggtg ggcggcgaca acccgtacat cgagccccag
     2581 catctgctgc tcgcgctcct ggatcaggag gacggcggca g
//
LOCUS       DASPUL010000481         4991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_923927, whole genome shotgun sequence.
ACCESSION   DASPUL010000481 DASPUL010000000
VERSION     DASPUL010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923927"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VEJ00_11430"
     CDS             <1..742
                     /locus_tag="VEJ00_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01820.1"
                     /translation="LPAGSGTVSAMEVLMRSRSVVFTLSLYLSLVVLGCTSKPSTDNT
                     TNPAAGQNNTAGQESAGNEAKEHKEAREEKKKEPLVVPAGTSVTVSLGSALGSKLSQA
                     GQTFAATVAKDVMVGNEVAIPKGANVSGTVADAKPLGKFAGGAVLQVRLDSISIKGSD
                     VPVQTALRSFSAKGKGKRTAVMSGGGAAVGGIIGALAGGGKGAAIGMLAGGGAGAGGA
                     AFTGNKDIVLPAESDLTFEITQQIEVKR"
     gene            complement(829..1455)
                     /locus_tag="VEJ00_11435"
     CDS             complement(829..1455)
                     /locus_tag="VEJ00_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXX01821.1"
                     /translation="MHDHVLLRQGLRRLLEDEADVEIVAEAGNATEALQGISQHLPDV
                     VITDARVFDYTAESVEQMILEESPQTKVLFLTAREDANPLPEARGDEASCAVRRTSAE
                     ELVEMLRRVHSREPAGPREMTRRWRVEGPPEELLSRKRALTAREHEVLKLLAEGRTVR
                     SVAGVLGVSVKTVDAHKFNLMRKLGIHNKAELVMWAIQQKVVKLPVNF"
     gene            1725..2897
                     /gene="aroC"
                     /locus_tag="VEJ00_11440"
     CDS             1725..2897
                     /gene="aroC"
                     /locus_tag="VEJ00_11440"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXX01822.1"
                     /translation="MLRYFTAGESHGEALVAFLSGIPAGLKIDQDFLNRELWRRQQGY
                     GRGGRMKIERDTAHILSGVRQGLAIGSPIAVLLENKDWKNWQESLPVVAGDPAKHKRV
                     ASPRPGHADLAGALKYNFPEARYVLERASARESAARVAIGAIAKLLLRELGIEVLSHV
                     VTVGNVVVSDQVPWEKICALREREEVLLNCADPEAESRMKDEVDKVLKTGDSLGGIFE
                     VVAHHVPPGLGTYAQWDERLDAQLAAAVMSLQAVKAVEIGAGVAAAFAPGSAVHDEIG
                     YSATTGFTAFSRTRNNAGGIEGGVSNGEEIRVRGYLKPISTLRRPLQSVDFATREPVK
                     AAYERSDVCVVPAAGVAAEAMVALTLARCALEKFGGDSMTETLRNFQGYCQQLKNY"
     gene            2914..3423
                     /gene="def"
                     /locus_tag="VEJ00_11445"
     CDS             2914..3423
                     /gene="def"
                     /locus_tag="VEJ00_11445"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXX01823.1"
                     /translation="MIYPIVKFGNPVLEKPSETVTLFDEELQKLVDDMFESMYAAHGV
                     GLAAPQIGIGRRIAVIDVTFKEDPKAKLVLINPEIIHAEGKHTQSEGCLSIPDFRENV
                     SRPKKVTVRAQDVHGKVFEKTGEELLARALLHETDHLNGKLYISHLSALKRDLIKRRI
                     RKMIKAGEW"
     gene            3475..4422
                     /gene="fmt"
                     /locus_tag="VEJ00_11450"
     CDS             3475..4422
                     /gene="fmt"
                     /locus_tag="VEJ00_11450"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXX01824.1"
                     /translation="MSEPLNLVFCGTPRFAVPTLEKLVAAGSRISLIVTQPDRPKGRG
                     LELVGSPVKDSALKLNLPITQPDRIKNNEEFRSRLTIIQPDAIIVVGYGRLIPQWMLD
                     LPRFGNINLHASLLPKYRGAAPVQWAIANGETATGVTTMRLDVGLDTGDILLQQAIPI
                     APDDTAETLAPRLALLGADLLIETLEGLQAGNIQPRPQDNSQATLAPILKKEDGLIDF
                     SRPATETVNRLRGFQPWPGAYTAFRGKNLQILQAKPSHAALPSAELSVDANRLLVGCG
                     QNTCLEIIELQLEGKKRTPAQAFIQGYRPKPGEKLGVSS"
     gene            4581..>4991
                     /locus_tag="VEJ00_11455"
     CDS             4581..>4991
                     /locus_tag="VEJ00_11455"
                     /inference="COORDINATES: protein motif:HMM:NF013216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXX01825.1"
                     /translation="MPVSPARAAAFDILLRVECESSYASELLHSAACERLSPSDHGLA
                     TELVMGVLRWRSRLDDEIATASSQALPKLDLEVLISLRLALYQLRRLDRIPQRAALHE
                     SVELVKRARKRSAVPFVNAVLRRLSAAPPDRDAPA"
BASE COUNT          983 a         1613 c         1410 g          985 t
ORIGIN      
        1 cctgcccgct ggttccggta cagtttcagc gatggaggtg cttatgcgtt cccgctcggt
       61 tgtattcacc ctgagtttgt acctctcgct ggtcgtgctc ggatgcacca gcaaaccctc
      121 gacggataac accaccaatc ctgccgcagg ccaaaacaat actgccggcc aggagtcggc
      181 cggcaacgaa gccaaagaac acaaggaagc ccgggaggaa aagaagaagg aaccgctggt
      241 cgtcccagca ggaaccagcg tgactgtcag cctcggttca gccctgggtt ccaagttaag
      301 tcaggcggga cagacctttg ctgctacagt cgccaaagat gtgatggtgg gaaacgaagt
      361 cgccatcccg aaaggggcga acgtgagcgg cacggtagcc gatgccaagc ccctgggtaa
      421 gttcgccggc ggcgcggttc tgcaagtgcg cctcgactcc atcagcatca agggctcaga
      481 tgtcccggtt caaactgcgc tgcgctcgtt ctcggccaag ggcaaaggca agcgcactgc
      541 cgttatgtcc ggcggcggcg ccgcggtcgg tggcatcatc ggagccctcg cgggtggcgg
      601 caaaggcgct gcgatcggca tgctggccgg cggtggcgcg ggagccggtg gcgctgcctt
      661 caccggaaac aaagatatcg tgctgcccgc cgagtcggac ctgaccttcg agatcacgca
      721 gcaaatcgaa gtcaaacgat agacgacagg gacgtcgtcg gaggctcagc ttcgagcgct
      781 cagcatcgat agtcaagctg tcgggaatgt ttgccgaagc aaccgaccct aaaagttcac
      841 cggcagcttg accactttct gctgaatcgc ccacatcacc agctccgcct tgttgtgaat
      901 tcccagcttg cgcatcagat taaacttgtg cgcatccacg gtcttgacgc taaccccgag
      961 aacgccggcc accgaacgca cggttctgcc ttccgccagc agtttcagga cttcgtgctc
     1021 gcgagctgtc aaggcacgtt ttcgggaaag caattcctca ggcggtccct cgacgcgcca
     1081 ccgtcgcgtc atttcacgtg gcccggcggg ctccctggaa tgaactctcc tgagcatttc
     1141 caccagttct tcagcggagg tccgccgcac ggcgcaactc gcttcgtcgc cccgcgcctc
     1201 aggcaagggg ttcgcgtcct cccgcgcggt caggaacagc actttggtct gtggcgattc
     1261 ttccaggatc atctgctcaa cgctctcggc ggtgtagtcg aacactcgcg cgtccgtaat
     1321 cacgacgtca ggcagatgct gactgattcc ttgtagcgct tccgttgcat tccctgcttc
     1381 tgccacgatt tccacgtccg cctcatcttc aagaaggcgc ctcaatccct ggcgcagcag
     1441 gacgtggtcg tgcacgagca agcagcgaat tttggtacta ctggaaaatt ctggtgggat
     1501 ctgtgcgtgg gggcacacag ttcctcctcc tacggccaga gagatttgtc acgcagcgcg
     1561 ggttcctcga acccagacgg agggcgagac gcgatgacat tgtaacctgc cgcgaggtgg
     1621 tgtggctgtg aaaatccccg tccagtgccg tttttgggac tagtcagcct acgctgggag
     1681 gcgtaatccc gattgcgcca cccatcaggt acaatcgagc gtttatgttg cgctacttca
     1741 ctgcgggaga atcccacggc gaagctctgg tcgctttcct ctccggcatc cccgctgggt
     1801 taaaaatcga ccaggacttc ctcaatcggg aactctggcg ccggcagcag ggatatggcc
     1861 gcggcggccg catgaaaatc gagcgcgaca ccgcacacat tctttccggc gtgcgccagg
     1921 gactggccat cggctctccg attgccgtcc ttctcgaaaa caaggattgg aagaactggc
     1981 aggagtcttt gccggtcgtc gccggcgatc ccgccaagca caagcgcgtt gcctctcccc
     2041 gccccggcca cgccgatctc gcgggtgcgc taaagtacaa cttccccgag gcccgttacg
     2101 tcctggagcg tgcttcggcg cgcgagtcgg ccgcccgggt ggcgattgga gccatcgcca
     2161 agctcttgct ccgcgaactc ggaatcgaag tgctgagcca cgtggtgacg gttggcaacg
     2221 ttgtggtctc ggatcaggtt ccgtgggaaa aaatctgcgc tctccgcgag cgcgaggaag
     2281 ttctcctcaa ctgcgccgat cccgaagccg agtcgcgtat gaaagacgaa gtcgacaagg
     2341 tgctcaagac cggagactcg ctgggcggaa tctttgaggt cgtggctcac cacgttcctc
     2401 cggggctggg cacctacgcc cagtgggacg agcgtctgga tgcgcagctc gccgccgcag
     2461 tcatgtctct gcaggccgtg aaggcagtcg agatcggtgc cggcgtcgca gccgctttcg
     2521 ctcccggctc tgccgtacac gacgagattg gctattcggc gaccacaggc ttcacagcct
     2581 tttcccgcac ccgcaacaac gccggcggca ttgaaggcgg cgtctccaac ggagaagaaa
     2641 tccgtgtccg tggatacctc aaaccgatct ccacgctgcg ccgtcccttg cagtcggtcg
     2701 atttcgcgac gcgggagccg gtcaaggccg cgtatgagcg ttccgatgtt tgcgtcgtgc
     2761 cggctgccgg tgtggctgcc gaggccatgg ttgcgctcac cctggcgcgc tgcgcgctgg
     2821 agaaattcgg tggcgattcc atgactgaga ctttgaggaa tttccagggc tattgccagc
     2881 aattgaaaaa ctactgatga tcgaccgcat cgcatgatct accccatcgt gaagttcggc
     2941 aatccggttc tcgagaaacc ttcggaaacg gttaccctct tcgatgagga actacagaag
     3001 ctcgtcgacg acatgttcga gtcgatgtac gccgcccacg gtgtcggcct ggccgcgccg
     3061 caaatcggca ttggccgccg cattgccgtc atcgacgtca cgttcaagga agatccaaaa
     3121 gccaagctcg tcctcatcaa tcctgaaatc attcacgccg agggcaagca cacccagagt
     3181 gagggatgcc tcagcatccc cgactttcgc gagaatgtca gtcgcccgaa gaaggtcacc
     3241 gtccgcgctc aggatgttca cggcaaggtt ttcgaaaaga ccggcgagga attgctggcc
     3301 cgtgccctgc tccacgaaac cgatcacctg aacggcaagc tctacatcag ccacttgagc
     3361 gcattgaaac gcgacctgat caaacgcaga atccgcaaga tgatcaaagc gggagaatgg
     3421 tagacattga gtcactgaat catttactca tggactcaac gagtcggtga ttgaatgagt
     3481 gaacccctca atctcgtctt ctgcggcacg ccgcgtttcg ccgtccccac gctggaaaag
     3541 cttgttgccg ctggatctcg catctccctc atagtgactc agcccgatcg ccccaaaggc
     3601 cgcgggctgg aactcgtggg ctcgccggta aaggactccg ccctcaaact gaatcttccc
     3661 atcacccagc cggaccgaat caagaacaac gaagaattcc gctcccggct cactatcatc
     3721 cagccggacg ccatcattgt cgtcggctac ggccgcctga ttccgcagtg gatgctcgat
     3781 ctgccccgct ttggcaatat caatcttcac gcgtcgctgc tccccaaata ccgtggtgcc
     3841 gctcccgtcc agtgggccat cgcaaacggc gagaccgcga cgggcgttac cacaatgcgc
     3901 ttagacgtcg gcctcgacac cggtgacatc ctccttcagc aagcgatccc catcgctccc
     3961 gacgatacag ctgagaccct cgctccacgg ctcgccctgc tgggcgcgga tctcctgatc
     4021 gagacactgg aagggctgca agcgggcaat atccagcctc gcccgcaaga caactcgcaa
     4081 gcgaccctgg ccccgattct caagaaggag gacggcctca tcgatttctc gcgcccggcc
     4141 acggaaaccg tcaatcgtct tcgcggcttc caaccctggc ccggagcgta cacagcgttc
     4201 cgtggcaaaa atctgcaaat cttgcaggca aagccgtccc acgccgcgct cccatccgcg
     4261 gagctgagtg tcgatgcgaa ccgactcctg gtgggatgcg gccaaaacac gtgtttagaa
     4321 atcatcgagc tccaactgga aggaaagaag cgaacccccg cccaggcctt tatccagggc
     4381 tatcgcccca agcccggcga aaagctggga gtctctagct gaacgttgct tccctggttt
     4441 ccttagcgtt tctttgcggc ctttcttcgc gatctccgcg gtaaatggtt ctgtttctcc
     4501 cattccgtct taagaacctc tcggacgctc tttggtgaag gccctgtcgc cgagcgtctc
     4561 aggcccctac aatgaacgga atgcccgtct ctcctgctcg cgccgccgct tttgacatat
     4621 tgctgcgcgt cgagtgcgag tcctcgtatg cttctgaact gctgcactcc gcagcctgcg
     4681 agcgtctttc cccttccgat cacggactcg ccaccgaact tgttatgggt gtcctccgct
     4741 ggcgttctcg cctcgacgac gagatcgcca ctgcgtcttc acaggccttg cccaaactcg
     4801 acctcgaggt cctgatttcg ctgcgcctgg cgctctacca gctccgccgg ctcgaccgca
     4861 tcccgcagcg agccgcgctc cacgaaagcg tcgaactggt caaacgtgcc cgcaagcggt
     4921 ccgcggttcc cttcgtaaat gcggtgctgc gcaggctatc agccgcaccg ccggatcgcg
     4981 acgcgcctgc a
//
LOCUS       DASPUL010000482         3910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1185660, whole genome shotgun sequence.
ACCESSION   DASPUL010000482 DASPUL010000000
VERSION     DASPUL010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185660"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1400
                     /locus_tag="VEJ00_11460"
     CDS             <1..1400
                     /locus_tag="VEJ00_11460"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXX01826.1"
                     /translation="VGEALGFSCIEASDGEAALALLEEQPAHMVLTDMVMPNMSGLEF
                     LEKVKKLLPRTEVALMTGHGSIETAVQAMKLGAYDYITKPFSPLEELRLFLRRMAEKV
                     RLVEENEFLRQRMDSETAVHGIVGSSARIQEVLRMVSRLKDTRTPVLIYGESGTGKEL
                     VARAIHFRGAFAARPFVAVDCGSLVPTLIESELFGYEKGAFTGALKSKQGLFQTADSG
                     TIFLDEVGELPLELQAKLLRVLQEKEVRPVGSNQRVKVDVRVIAATNRDLEAAYKAGT
                     FRKDLYFRLNVVTMHVPALRERRSDIPMLVHWFLERYAPGAGQQVSSAAMKSLMQYDW
                     PGNVRELENCIERAVALGNGQIIDLGDLPPAIAAGSAVLPAGAAAQRPPAFDPDLAAV
                     VPASASPEAPLSTTDLEDIERATIQRVFEQVKGDKALAGRMLGISRATLYRKLKRYNI
                     GMGSSGPGSSSHTLQ"
     gene            complement(1546..3057)
                     /locus_tag="VEJ00_11465"
     CDS             complement(1546..3057)
                     /locus_tag="VEJ00_11465"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXX01827.1"
                     /translation="MKVLTVRVSLCVFLCLFGLNGAHAQTTGSFKHIVLIVQENRTPD
                     NLFHGLLTWPGINPANYDIATSGLALVNGKDEVVALTPGPLANDYDLSHAHPSFLIMY
                     DKGKMDRANLIPDTCEKTAIDCTSNGAGQFLSYKYVDNSTHIVDPYLQLAANYGWANQ
                     MFQTNQGPSYPAHMFLFGGTSALTAADDAAGTFMSENPGSPTGSDYSATQDTGCLAPS
                     GEFNFLVTAGSTTETKLINTVGTFCVTHETMASLLNGIGVSWKYYAPAVLTNSFPNDP
                     ARKGYNSGGSIWNAPASISPICQPDSAFAACAGAEYTSKVDLNPPDVLTDISECKLSG
                     VSWVIPDGRNSDHPSMNQGGGPSWVASIVNAIGENTTCDGKGYWQDTAIIVTWDDWGG
                     WFDHVKPPVLSGPQGDYQMGFRVPLIFISAFTTPAAVSDVRYDFGSVLRFVEKNFKLR
                     EGELGFADARSDTNLGEFYDFRLGPLPFRPVAAPLKASFFLSQTGPPEPPDTD"
     gene            3520..3885
                     /locus_tag="VEJ00_11470"
     CDS             3520..3885
                     /locus_tag="VEJ00_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01828.1"
                     /translation="MAQPAAATARNAQNVGAVAGLARFFPEAVPVRIPVQLARTLQAG
                     APAAASFSESTVIEFGTTREVLFACNTPLEFADVLRLRNSDGSLDIEASVVAVQYHPG
                     QTVVAARFLGEVPNWIVKP"
BASE COUNT          756 a         1222 c         1194 g          738 t
ORIGIN      
        1 ccgtgggcga ggcgctgggg ttttcctgta ttgaggcctc cgacggcgag gccgcgctgg
       61 cgctgctgga agagcaaccc gcgcacatgg tgctcaccga tatggtgatg cccaacatgt
      121 caggcctgga gttcctcgag aaagtgaaga agctgctgcc ccgcaccgaa gtcgcgctca
      181 tgaccggaca cggctcgatc gagaccgcgg tgcaggccat gaagctgggc gcctacgact
      241 acatcaccaa gccgttttct ccgctcgaag agctgcgcct gttcctgagg cgcatggcgg
      301 aaaaagtccg cctggtggaa gagaacgaat tcctgcgcca gcgcatggat tcggaaaccg
      361 ccgtgcacgg cattgtgggc tcctccgccc gtatccagga agttctgcgc atggtctctc
      421 gcttgaaaga cacgcgcacg ccggtgctca tctacggcga aagcggaacc ggcaaggagc
      481 tcgtcgctcg cgccatccac ttccgcggcg ccttcgcagc gcgcccgttc gtggccgtcg
      541 actgcggatc gctcgtcccc acgttgattg aaagcgagct gttcggctac gaaaaaggcg
      601 ctttcactgg cgcactgaag tcaaagcagg gattgttcca gaccgccgac agcggcacca
      661 tctttctcga tgaggttggc gagttgccgc tcgaactgca ggcaaagctg ttgcgcgtgc
      721 tgcaggaaaa agaagttcgt ccggtgggaa gcaaccaacg tgtgaaggtc gatgtgcgcg
      781 tcatcgctgc caccaaccgc gatctcgaag ctgcctacaa agccggaacc ttccggaagg
      841 atctctactt ccgcctcaac gtcgtcacca tgcatgtgcc cgcgctgcgc gagcggcgca
      901 gcgatatccc catgctggtg cactggttcc tcgagcgcta tgcacccggc gccggccagc
      961 aggtctccag cgccgccatg aagtcgctga tgcaatacga ctggccgggc aatgttcgcg
     1021 agctggagaa ctgcatcgaa cgcgcggtcg cactgggcaa tggacagatc atcgacctcg
     1081 gcgacttgcc gccggcaatc gccgcgggct ctgcggtgtt gccggcgggt gcggccgcac
     1141 agcgaccgcc cgcgtttgat ccggacctcg ctgcggtggt tccggcttcc gcatcccccg
     1201 aagctccctt gtcgaccacc gaccttgagg atatcgagcg cgccaccatc cagcgcgtct
     1261 tcgaacaagt gaaaggcgac aaggccctcg ccggacgcat gctcggcatc agccgcgcca
     1321 cgctctaccg caagctgaag cgctacaaca tcggtatggg ttcgtcgggg ccgggatcgt
     1381 cgagccacac gctgcagtag ggactggccg ccttcccgct cgaacttcca ccagtgcatg
     1441 aacttccaaa gccaccccag cgaatcggct ttgaaagggc gcgccttcag gcgcgccgta
     1501 gaaatgccaa agcgaggggg ctttagcccc ctgaaggaat agcgcctagt ccgtgtccgg
     1561 cggctccgga ggcccggtct ggctcaggaa gaagctcgcc ttcagcgggg cggccaccgg
     1621 acgaaaggga agaggaccca gccggaaatc gtagaactcg cccagattcg tatccgagcg
     1681 ggcatcggcg aagcccaact cgccctcgcg cagcttgaag ttcttctcca cgaagcggag
     1741 cacggaacca aaatcgtagc gcacgtcgct caccgccgcc ggggtcgtga acgccgagat
     1801 gaaaatcaag ggcacgcgaa accccatctg atagtcaccc tgcggcccgc tcagcaccgg
     1861 aggcttcacg tgatcgaacc acccgcccca gtcgtcccag gtcacgatga tcgccgtgtc
     1921 ttgccaatag cctttgccgt cgcaggtggt gttctcaccg atggcgttga cgatcgaagc
     1981 cacccaggaa ggtccgcccc cctgattcat gctggggtgg tcggagttcc ttccatcggg
     2041 aatcacccag ctcacccctg acagtttgca ctcactgatg tcggtgagca cgtccggagg
     2101 attcaaatca accttgctcg tgtattcagc cccggcgcac gccgcaaaag ccgaatcagg
     2161 ctggcaaatc ggagagatcg aggcgggtgc gttccagatg gacccgcccg agttatatcc
     2221 ctttcgcgcc ggatcgttgg gaaaggaatt ggtcaacacc gcgggagcgt agtatttcca
     2281 gctcacgccg atgccgttca gcagtgacgc catggtttcg tgcgtcacgc aaaatgtgcc
     2341 caccgtgttg atgagcttgg tctcggtggt cgagccggcg gtgaccagga agttgaattc
     2401 accgctcggt gccaggcacc cggtgtcctg ggtagcggaa tagtcgctgc ccgtcggact
     2461 tcccggattt tcgctcatga acgtacccgc tgcgtcatcg gcggcggtga gcgccgatgt
     2521 gcctccgaac agaaacatat gcgccgggta gctcggtccc tgattggtct ggaacatctg
     2581 gttggcccag ccgtagttcg ccgccagttg cagatacggg tcgacgatgt gcgtgctgtt
     2641 gtcgacgtac ttgtacgaca ggaactgccc tgcgccgttg ctggtgcagt cgatcgctgt
     2701 cttctcgcaa gtgtcgggga tcagattggc ccggtccatc ttgcccttgt catacatgat
     2761 cagaaacgac gggtgggcgt gcgacaggtc gtagtcgttg gccagcgggc ccggagtcag
     2821 cgccaccact tcgtcttttc cgttgaccag cgccaggccg ctggtcgcaa tgtcgtagtt
     2881 tgccgggttg atgcccggcc aggtcagcaa gccatgaaac aggttgtcgg gtgttctgtt
     2941 ttcctgcacg atcagcacaa tatgtttgaa ggaaccagtg gtctgggcat gagctccgtt
     3001 tagtccgaaa agacacaaaa agacgcagag agagacacgt accgtaagaa ctttcacggg
     3061 acttaaacct ccagagggga aggaaacttt cggggtgtca ccgaccgcga agcaaggggg
     3121 attttcagcc aatctaccac aagtcttgcc ggcgcaccat acggtgggca caggggaaag
     3181 gcgcgatccg cgggcgctcc cgccgcttga tcggcaaacg ctttgccgga aacgacttga
     3241 gacacgtgtc tcgggttgag acagatccgg ggctcgcctg ccattcccgc ctcatgattc
     3301 cgctgcaact tactgaaaat cctttcactc gggtgctagc cctttcgttc gcagctttgg
     3361 ttgaggtcgt gctcggtaat tgggcgtaac cgcggattct cggctcccca aagctcttcc
     3421 taaccgggaa gagggggagc ccgaccgcgt ttgcgcgaag agcagatcgc aacatttcgt
     3481 agttcagagc ttgcaattga gggcttgagg ggtttgggct tggcacaacc ggccgcagca
     3541 acggcacgca acgcgcagaa cgtgggcgcg gtggccggcc ttgcccggtt ttttcccgag
     3601 gccgtgccgg tgcgcatccc ggtgcagctg gcgcgtacgc tgcaggccgg cgcgcccgca
     3661 gccgccagct tcagcgagag cacggtcatt gaattcggca ccacccgcga agtcctcttc
     3721 gcctgcaaca ccccgctgga gttcgccgac gtgctgcgcc tgcgcaactc cgacggttcg
     3781 ctcgatatcg aagcctcggt cgtcgccgtg cagtatcatc ccggccagac ggtcgtggcg
     3841 gcacgctttc tgggcgaagt gcccaactgg atcgtgaaac catgaccgaa cccacacctc
     3901 cacaagatct
//
LOCUS       DASPUL010000483         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_88204, whole genome shotgun sequence.
ACCESSION   DASPUL010000483 DASPUL010000000
VERSION     DASPUL010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88204"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..684
                     /locus_tag="VEJ00_11475"
     CDS             172..684
                     /locus_tag="VEJ00_11475"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HXX01829.1"
                     /translation="MNQIAAASAPAADSLKRRLACEQLRVSGKVQLRATGSSMLPSIW
                     PGDVLVVEQVRNAGILRGDVVLFDAGRRFVAHRVIARDRSTIRTQGDSVPKPDRPLSS
                     DELLGKVAFILRNGKCVLLSKNVRASDRAMAALFRRSQLATRVAVGVHSLRRASKDHN
                     SNHRAVPCQS"
     gene            735..1487
                     /locus_tag="VEJ00_11480"
     CDS             735..1487
                     /locus_tag="VEJ00_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01830.1"
                     /translation="MRVNTADPVFLGMLQDRYTGFVGNSVQAEIDFEVELVPPGFSDP
                     DTDVRVTNLQGRWLLRRNDFIAEWDPAAGKGWIRQTANPYSIDSVLRIVHTLVLAREG
                     GFLLHSASGVRNGKAFLFAGVSGAGKTTISRLAPPDVTLLTDEISYVRNLEQGYFAFG
                     TPFAGELAKLGKNVSAPIAALYLLAKGRENRIDPVAPGEAARSLLANMLFFAEDEHLV
                     QGAFHSAFEFVSRVPVSRLTFVPDARVWELIG"
     gene            1517..1807
                     /locus_tag="VEJ00_11485"
     CDS             1517..1807
                     /locus_tag="VEJ00_11485"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HXX01831.1"
                     /translation="MAARCLDGEMMIMSGRDSTLFTLNKTATILWQAADGNTSLEEIV
                     EQKICAEFEVEPAEALQDAENLARELASHEILQISEEPIPQPNSTTPPGGLR"
     gene            1804..2946
                     /locus_tag="VEJ00_11490"
     CDS             1804..2946
                     /locus_tag="VEJ00_11490"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXX01832.1"
                     /translation="MSGLLVEMTEKALARNIPLSVQLDLTYRCNERCVHCYLDHDDHG
                     EMTTAEIKHLLEEMADAGVFILTLSGGEIFLRKDFFELLEYARRELTFCVKLKTNAIM
                     IREREAARLRDLGVQSIQISIYSHRPDVHDAITLVPGSLKRSIDAIRFLKSQGLRVIM
                     ANVLMVQNLQDYQGVRALADELGVEATLDPTITPMMDGDRSVLSMGVDQTALQRVFRD
                     KALVGDVDEFCAIPAKADEGAIESLPCSAGHTTCYVSPYGDVYPCVQFPYPTGNVRQQ
                     RFLDIWRYSDRMNEVRAIRLKDLTGCSSCVHGGSCTRCPGLAFMEGNMRGPSTQDCEK
                     SFAKTGVPSANLLAKRITDHNSVHTGSAAPKLVQIQMLPDLARATA"
BASE COUNT          597 a          949 c          902 g          642 t
ORIGIN      
        1 gttttctatg actatgaaag ataccagcac agaccagtcc actaccgcga ccgccaggaa
       61 gccttacgag aagccgacct tccgtcatga agaggtgttc gtgacgacag cgctcggctg
      121 cggaaagatc actcaaaccc agtcgggctg cggcttcagt caaaaggcgt catgaatcaa
      181 attgccgccg cttccgcccc ggccgcggat tccctgaagc gccgattggc ctgtgaacag
      241 ttgcgcgtct cgggaaaggt tcagctgcgt gctactggct caagcatgct gccttccatt
      301 tggccgggcg atgtgctggt tgttgagcag gtccgcaatg ccggcatttt gaggggagat
      361 gtcgtgctgt tcgatgcggg gaggcgattc gttgcgcacc gggtgatcgc ccgcgaccgt
      421 tcgacaattc gcacccaggg cgattcggtg cctaagccgg accggccgct ctcgagcgat
      481 gagctgctgg ggaaggttgc tttcattttg cggaatggaa agtgcgtact gctgagcaag
      541 aatgtgcgcg cgtcggaccg tgcaatggcg gcgctttttc gccgatcgca actcgccact
      601 cgcgtggctg tgggtgttca cagcctgcgc cgagcttcaa aagatcacaa ttcaaatcat
      661 cgagccgtcc cttgccagag ttagccgctg aagtcgaacg tctcagcctt gtcgttgaag
      721 tcggcggtct ccctgtgcgc gtgaacaccg cggacccggt ttttctgggc atgctgcagg
      781 accggtatac aggcttcgtg ggcaactctg tacaggcgga gatcgacttc gaggtcgagc
      841 tggtgccgcc cggcttctct gaccctgaca ccgatgtgcg cgtgacgaac cttcagggcc
      901 ggtggttact tcgccgcaac gacttcatcg ccgagtggga tccggcagct ggtaaaggtt
      961 ggattcgcca gacggccaac ccctattcca tcgactccgt gctgcggatc gtgcacaccc
     1021 tggtgcttgc tcgggagggc ggattcctgt tgcattccgc cagcggcgtg cgcaatggca
     1081 aggcattcct gttcgccggc gtctccgggg cgggcaagac cacaatctcg cgcctcgctc
     1141 ctcccgatgt cacgcttctg accgatgaga tctcttacgt acgcaacctg gagcaaggat
     1201 atttcgcatt cggcaccccc tttgccggag aactggccaa gctgggtaag aacgtatcgg
     1261 ctcccattgc cgctctctac ctgctcgcca aagggcgcga aaaccgcatt gacccggtgg
     1321 cccccggcga ggcagcccgg tctctgcttg ccaacatgct ctttttcgcg gaagatgagc
     1381 acctggtgca gggcgctttt cattccgcct tcgagttcgt cagccgcgtc ccggtttcgc
     1441 ggttaacctt tgtgcctgat gcccgagtgt gggagctgat cggatgagcc gtcttttcgt
     1501 agcacggagt ccaggagtgg cggcgcgctg tctcgacggc gaaatgatga tcatgtccgg
     1561 gcgcgactcc actcttttca cgctcaacaa gaccgccacc atcctctggc aggcggccga
     1621 cgggaacacc tcgctcgagg aaattgttga acagaaaatc tgtgcagaat tcgaagttga
     1681 gcccgccgaa gccttacagg atgccgagaa cctggctcgt gagctcgcca gccacgagat
     1741 tctgcagatc tccgaagagc ctattccgca accaaactct acaactccac cgggaggact
     1801 ccgatgagcg ggctcctcgt ggagatgacc gagaaagcgc tggcccgcaa cattccgctg
     1861 agcgtccagc tcgatctcac gtaccggtgc aacgagcgct gcgtacactg ctatctcgac
     1921 cacgacgatc atggcgagat gaccacggcc gagatcaagc acctcctcga agaaatggcc
     1981 gacgccggcg ttttcatcct gaccctcagc ggcggcgaaa ttttcctgcg caaagacttc
     2041 ttcgagcttc tggaatatgc gcggcgagag ctgactttct gcgtcaagct caagaccaat
     2101 gcgatcatga tccgcgagcg ggaagcggcc cggctccggg atctcggcgt acagtcgatc
     2161 cagatcagca tttactcgca ccggcctgac gttcacgacg ccatcaccct ggttcccggt
     2221 tctttgaagc gctcgatcga tgccatccgc tttctcaagt ctcaagggct gcgcgtcatc
     2281 atggctaacg ttctgatggt ccagaatctg caggattatc agggtgttcg cgccctggcc
     2341 gacgaactgg gcgtcgaagc aacgctcgac cccacgatca cccccatgat ggatggagac
     2401 cgctcggtcc tgagcatggg agttgaccag accgctctgc agcgggtctt tcgggacaag
     2461 gcgctggtgg gcgatgtcga cgaattctgc gcgatcccgg caaaggcgga cgagggcgcg
     2521 atcgagagtt tgccctgcag cgcgggtcat accacctgct atgtctcgcc ttacggcgac
     2581 gtctatccct gtgtgcagtt tccctatccc acaggaaacg tgcggcagca gcgctttctc
     2641 gacatctggc gctattccga ccgcatgaac gaagtgcggg cgatccggct gaaagatctt
     2701 acaggttgtt cctcgtgcgt gcatggcgga agctgcacgc gctgtccggg cctggctttc
     2761 atggaaggaa acatgcgcgg accttcgact caggactgcg agaagtcgtt cgcgaagacc
     2821 ggagtcccgt ccgcaaatct cctggcaaaa agaatcacgg atcacaactc ggtgcacacc
     2881 ggttccgcag caccgaagct cgtgcagatt caaatgcttc ctgacttagc ccgcgccacc
     2941 gcttgacccg gactcactcc tgctggtcct atggagagca tgtccgggac tggcgccatg
     3001 tccgacctcg agtggaccat actcatcgcc gcgtgttccg ctatttccgg tcctgacaag
     3061 tcagccagga tccagcccct gcttcagcaa
//
LOCUS       DASPUL010000484         3900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1185785, whole genome shotgun sequence.
ACCESSION   DASPUL010000484 DASPUL010000000
VERSION     DASPUL010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185785"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="VEJ00_11495"
     CDS             complement(<1..977)
                     /locus_tag="VEJ00_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01833.1"
                     /translation="MLPLPNSGNVTLTLDEYNKLVELAAKPPKKPDVAPLPYSIKHAD
                     VQLRVEKDEVLGTVQMEGEVFRKGVSKVPLTSGMTILDAHQLEAHQASKDLPLEQESG
                     THIALLSGPGEFSVDLETGLPLRIEAGRASFSLPVPAAGSVQLTLVIPGDHTWANISP
                     GLITSRKSENGHTAVEATLVPGQPASIWWATREAPAPVAPREVRFLADEKTLVSVSEA
                     EMRVAVLADLTVVQGEPAQFHVEVPEGYEITGVTGASLDSTETHSGVLTLKVNAPGHN
                     NHQFLISMEKSINDNKAEAPFLSFKGAQRETGEVLIEGAGTMEITATEGG"
     gene            complement(1049..3076)
                     /locus_tag="VEJ00_11500"
     CDS             complement(1049..3076)
                     /locus_tag="VEJ00_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01834.1"
                     /translation="MNTSLLTVVPQLSSALRRFLLCLVAAATFFAGAIQAQEKAPEAS
                     RSAGWVVIPVEEYRVLRAKAYPVEPNPEPPPLDATLTRVDYDLRVAGEVAAGRASLTV
                     DVLKDGWVRVPVPAGLLVREARLDGKLVSLVPGAAGGKGGAHVSALLSHAGRSVLLLD
                     VDVPVSSSAGEETLSLPSAESGVTRATVQLPRLGIDLRVGGGLLSEKSEAPGETKWLA
                     YGRGNEALTFTWRRKTEDHHVDLPLRMRGSLTQLTSLAEDSSLIYAEANLEVVQGAAR
                     EVKIQLPEKVTINQVSGAMVADWEMKNGELAVTFLEPVEHSARFVISGETRLPRDGVI
                     DVPLLRLEGTERDEGGVAVEVLGAGEIKSQKPQGLDDADATDLGEMIAGRQSPALVAF
                     RVKPGEAGATRSLSVNIVRYDQQAVLMANVEEARYQILMSADGKELVQARYAVRNNQR
                     NFVKVTLPAGATVWSASLAGKPVRPGQAPDGSLLLPLEKSRGGDDAPAFAVEILYITK
                     AAAWEEKGRGKVTLPALDLPISRTGLLLYYPPLFHVTAETGTFRAGEYENPSAAALLP
                     PSVEVASDGRFTRSGLEHLVPNAGNTEFDRLEQFAQLQGNDKERKDSTQALLDTFRSK
                     SSAGKVTGILPVNVAFPAFGPSVFLVSELTSENQAPAAEFSFQREKKGGVR"
     gene            complement(3300..>3900)
                     /locus_tag="VEJ00_11505"
     CDS             complement(3300..>3900)
                     /locus_tag="VEJ00_11505"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXX01835.1"
                     /translation="VETLLLPDKLFDGAVPSETPQGVAALVRLKEFTLDDVLERMQVG
                     PIVVVAGLQDPGNLGTILRSAEAFGSAGAVLGEGTVSPFNTKVIRASAGSVFRLPVIV
                     AKAAGGIEAIAARLRAGGVRLIATSSHKGTALDQANLVGASAVFIGSEGSGLPRSLAG
                     QMDETVAIPHRPQVESLNAGVAGSIVLYEAARQRRGHES"
BASE COUNT          691 a         1355 c         1090 g          764 t
ORIGIN      
        1 ccgccttcgg tggccgtaat ctccatggta cccgcgccct caatcagaac ctcgccggtt
       61 tcgcgttgcg ctcctttgaa gctgagaaac ggcgcttcgg ctttgttgtc gttgatcgat
      121 ttctccatcg agatcaggaa ctggtgattg ttgtgtcccg gagcgttgac tttcagagtc
      181 aaaactccgg aatgcgtttc ggtggaatcg agcgaagccc ccgtcacgcc cgtgatctca
      241 tatccctcgg ggacttccac gtgaaactgc gccggctcgc cttgcacgac ggtgaggtcg
      301 gcgagcacag ccacgcgcat ctccgcctcg ctcaccgaca cgagtgtttt ttcatccgcc
      361 aggaatcgca cctcgcgcgg cgccacgggc gccggagctt cacgcgttgc ccaccagatg
      421 ctcgcaggct gtccgggaac gagcgttgct tcgaccgccg tgtgcccgtt ctccgatttc
      481 cggctcgtga tcaatcccgg gctgatgttc gcccacgtgt gatcgccggg aattaccagc
      541 gtcagttgca cgctgcccgc ggccggcacg ggcaggctga acgaggcccg tccggcctcg
      601 atccgcagcg gcagcccggt ttcgagatcg accgagaatt cgccgggacc cgacagaagt
      661 gcgatgtgcg tgccgctttc ctgttcaagc ggaaggtcct tgctcgcctg atgcgcctcc
      721 agctggtgtg cgtcgagaat cgtcattccc gaggtgagcg gcactttgct cacacccttg
      781 cgaaagacct ctccttccat ttgcacggtg cccagcactt cgtctttttc cactcgcagc
      841 tgcacatcgg cgtgtttgat cgagtaggga agcggcgcga cgtcgggctt cttcggcggc
      901 ttggccgcca gttccaccag cttgttgtat tcgtcgagcg tcagcgtcac gttgcccgag
      961 ttggggaggg gaagcatcgt atccgatccg gcgtacgcaa gcactgagca aacactgcac
     1021 agcaacacga ttgagaatat tctgcctttc atcgcacacc tccctttttc tcgcgctgga
     1081 agctgaactc ggcggctgga gcctggttct cgctggtgag ttcggagaca agaaataccg
     1141 acggcccaaa cgcagggaat gccacattga ccggcaaaat acccgtgacc ttcccggccg
     1201 atgacttcga gcggaaggtg tcgagcaggg cctgggtgga gtccttcctt tccttgtcgt
     1261 tcccctgcag ttgggcaaac tgctccagcc tgtcgaactc ggtgttgcct gcgttcggaa
     1321 ccagatgttc cagccctgaa cgggtgaacc tcccatccga agcaacttcg accgaaggag
     1381 gcagcagcgc cgccgcactc ggattttcgt attcccccgc gcggaacgtt ccggtctccg
     1441 ccgtcacgtg aaacagcggc ggatagtaca gcagcagccc ggtgcgcgag atcggcagat
     1501 cgagcgcagg cagcgtcact ttgccacgcc ctttctcctc ccacgccgcc gccttcgtta
     1561 tgtacagaat ctcgaccgca aacgctgggg catcgtctcc gccgcgcgat ttttcaagcg
     1621 gcaagagcag gctgccgtcc ggcgcttggc ccggacgaac cggtttgccc gccagtgatg
     1681 cgctccacac ggtcgcgccc gctggcagcg tgaccttcac aaagttgcgc tggttgttgc
     1741 gaaccgcata gcgcgcctgc accagttcct tgccgtcggc gctcatcaga atctgatacc
     1801 gtgcctcctc aacgttcgcc atcaacaccg cctgctggtc gtagcgcaca atgttcacgc
     1861 tcaacgagcg cgtcgctccg gcttcgcccg gcttcacacg aaatgccacc agcgccggcg
     1921 actgccgccc tgcgatcatt tccccaagat cggtcgcgtc ggcgtcgtca aggccctgcg
     1981 gcttctggct cttgatctcc cccgcgccaa gaacttcgac ggccactccg ccttcgtcgc
     2041 gctcggtgcc ttccagccgc agcagcggaa catcgatcac cccgtcgcgc ggcagccgcg
     2101 tttctccgct gatgacaaag cgggcgctgt gctccaccgg ctccagaaaa gtgactgcca
     2161 gctcaccgtt cttcatctcc cagtccgcca ccatcgcgcc cgaaacctgg ttgatcgtga
     2221 ccttttccgg cagctggatt tttacctcgc gcgcggcgcc ttgcactacc tcgagattgg
     2281 cttccgcata aatcagactg gaatcttccg ccaggctggt cagttgcgtc agcgatcccc
     2341 gcatgcgcag cggcagatct acgtggtgat cttccgtttt cctgcgccag gtaaaagtca
     2401 aagcctcgtt gccccgcccg taggccagcc acttcgtctc tccgggcgcc tcagattttt
     2461 ccgaaagcag tcccccgccc acccgcagat caatccctaa ccgcggaagc tgcaccgtag
     2521 cgcgcgtcac ccccgattcc gcggaaggca gcgagagcgt ttcctcgccc gccgacgatg
     2581 agaccggaac gtcgacgtcc agcagcagca ccgaccgtcc tgcgtgcgac agaagcgccg
     2641 acacatgcgc tccgcctttc cctcctgccg cgcctggaac cagcgacacc agcttcccgt
     2701 cgagacgagc ctcacgcact agcagtccgg ccggcaccgg cacgcgcacc cagccgtctt
     2761 tcaagacgtc cacggtgaga ctggcccgcc ctgccgctac ttcgcccgcc acgcgcaggt
     2821 cgtagtccac gcgcgtcagg gttgcgtcga gcggtggagg ctcgggattc ggctcaaccg
     2881 gataagcctt cgcccgcagc acgcgatatt cttccaccgg aatcaccacc catcccgcgg
     2941 agcgggacgc ctccggagct ttctcttggg cttgaatcgc gcctgcgaaa aaagtcgcag
     3001 ccgcgacaag gcagagcaaa aatcgtctca gcgctgagct gagttgcgga accactgtaa
     3061 gaagcgacgt gttcatagat ccacctctgg agactcgatc gtttccaccg ttcaagcctc
     3121 acttcgatta gtcacgggga tgcgtagctc tccttaaggg atgtttcggg gtgggcgtcc
     3181 attcttccca agaacgcgct ctcgacggga agttgcgcgg gaatttgaaa tcagaaatgg
     3241 ccgggcttcc ggcctatcag ctaaagaggt taaggagtta cctgagtcac cacgcggcgc
     3301 tagctctcgt gtccacgccg ttgcctggcc gcctcataga gcacaatgct ccccgccacg
     3361 ccggcattga gcgactccac ctgcggccga tgcggaatcg ccacggtctc atccatctga
     3421 cccgccagac ttctcggcaa gcccgaccct tcgcttccga tgaagaccgc cgaggccccc
     3481 accagattcg cctggtcgag cgccgtcccc ttgtgtgatg aggtcgcaat caatcggaca
     3541 ccgcctgcgc gaagtctggc cgcaatcgcc tcgatcccgc cggcggcctt tgccacgatc
     3601 accggcaagc gaaacaccga tcccgccgat gcgcgaatca ccttcgtgtt gaacgggctg
     3661 accgtcccct ctccgagcac cgccccagcg ctgccaaagg cctcggccga gcgcagaatc
     3721 gtccccaggt tgcccggatc ctgcagtccg gccaccacga cgatcgggcc gacttgcatg
     3781 cgctcgagca catcgtcgag cgtgaattcc ttcaaccgca ccagcgccgc cacgccctgc
     3841 ggtgtctcgc tgggcacggc tccgtcgaac agcttgtcgg gcagcagcaa agtttcgacg
//
LOCUS       DASPUL010000485         6600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1735800, whole genome shotgun sequence.
ACCESSION   DASPUL010000485 DASPUL010000000
VERSION     DASPUL010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735800"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VEJ00_11510"
     CDS             complement(<1..248)
                     /locus_tag="VEJ00_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01836.1"
                     /translation="MSRDSSIDHQASTINSDLLSLADRLHSVAIHLLRRVRKQDVETG
                     EGPARLSALSVLVFGGPKTLGELAAAEQVKPPTMSRIVS"
     gene            316..708
                     /locus_tag="VEJ00_11515"
     CDS             316..708
                     /locus_tag="VEJ00_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXX01837.1"
                     /translation="MPMVETGIGIRKIGQIQLRAHDVERAAAFYENVLGLKLLFKAPP
                     NLAFFDCGGVRLMIDKPEKPEFDHPGSILYFTVPDIQGAHARLKEQGVRFEDEPHMIA
                     RMPDYDLWMTFFRDSEDNLMGLMSEVRR"
     gene            complement(829..1392)
                     /locus_tag="VEJ00_11520"
     CDS             complement(829..1392)
                     /locus_tag="VEJ00_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01838.1"
                     /translation="MITIVSGLPRSGTSLMMQMLVAGGMQALSDGERRADVDNPKGYL
                     EWERIKQLPNDPGCIAEAEGKVVKAISKLLLSLPAGHEYRVIFMQRPLPEVLKSQDEM
                     LRRRGTYKEGVDPAVIAAAFEKHLNEVYAWLDGKSYVKALRVPYHNVLSNPGEVAKQV
                     SDFLEIPLDVDAMTQQVDGSLYRNRAK"
     gene            1569..2666
                     /locus_tag="VEJ00_11525"
     CDS             1569..2666
                     /locus_tag="VEJ00_11525"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXX01839.1"
                     /translation="MGPRRTLHSTAVNLVVSAYVFLSVSCVLAQSSDPGSAVSDYVKA
                     EMQRQHIPGLSLLVIHNGKTVLAEGFGLANVELQVAVKPETVFQSGSMGKQFTATAVM
                     MLVEEGKVGLDDPLTKYFPEAPAAWKEVTVRELLSHTGGFGDYPKDFDLQKDVKEEDE
                     LKTFEQIPLAFTPGTKWEYSNLGYMTLGVLIHRVTGEFYGDFLQRRIFKPLDMQTTRI
                     ISEADIVPNRAAGYRLVKGELKNQEWVAPTINTTADGSLYFSILDLAKWDAALYTEKL
                     LKRSSLELMWTPVKLKDGKANKGDYGFGWVIVQRHGHRCITHDGAWQGFETTIDRYVD
                     DHLTVVALDNLSESQPSKIAEHVAELYLGEK"
     gene            2938..4404
                     /locus_tag="VEJ00_11530"
     CDS             2938..4404
                     /locus_tag="VEJ00_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXX01840.1"
                     /translation="MAAAGVTLKPAFGESQDLAYLTLKEASGLVRRKTVSPVELTATC
                     LKRIEAYNPALNAFITVTKGQAMETARAMEAERQQGKWRGALHGIPIALKDNIDTAGV
                     RTTAASELFKERVPSKDAEVVRRLKEAGAVLLGKTNMHEFALGGTSAVSYFGPVHNPW
                     ALDRIPGGSSGGSAAATAAGLCFGSLGTDTAGSVRIPASYCGIVGFRPTYGRVSSRGT
                     VPDSWTLDQVGPLCRTVEDAALMLGTIAAYDEFDPASVDVPVADYSLAFQMQVSQLRL
                     GIPRVPFFEGLDPEIEKATAAAIEVMGKLAAEVRDLTSSIVAAHLMEIWVKVAGAESY
                     TYHSHWLAESPEKYQAATRGRLLAPNNAGVQGENSTQMKASTYLEARRQLDVLRREIR
                     KVFANVDLLILPTMLAPPVTIAQGGDPTIVNPRNAASFSVLGLPALSLPSGFTTGGLP
                     IGLQIVGAPFAESTVLALAHAYERETEWHKRHPKVDPA"
     gene            4525..>6600
                     /gene="aceE"
                     /locus_tag="VEJ00_11535"
     CDS             4525..>6600
                     /gene="aceE"
                     /locus_tag="VEJ00_11535"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HXX01841.1"
                     /translation="MAHTETDGLEVLETREWVDSLDYVLEKGGPNRAGRLLQQLALHA
                     RREAGINLPFSATTPYQNTIPSRQQAPFPGSQEMERRIKSLVRWNALAMVVRANKIQE
                     GIGGHISTFASAATLYEVAFNHFLRAQTESGDRDVVYFQGHAAPGMYSRAYLEGRIPK
                     EKLQNFRRELKPGGGLSSYPHPWLMPDFWEFPTVSMGLGPIQAIYHARFIKYLENRGL
                     KQSTGGKVWAFLGDGEMDEPESLGSITLASRERLDNLVFVVNCNLQRLDGPVRGNGKI
                     IQELEAIFRGAGWNVIKVIWGTDWDNLIQNDRDGLLVKRLGEVSDGQYQKYFVESGAY
                     FRHNLFGTDPRLLKMVEHLSDEQLARMRLGGHDPIKVHAAYKAAVEHTGSPTIILAKT
                     IKGYGLGEAGEGKNITHQQKKLNDDELQMFRSRFGIPIPDDELHDAPFYRPADDSPEI
                     KYMEARRQQLGGYMPTRKVRATPLKPFPDSHFEEFHKGTEGREASTTMVFVRLLSKLL
                     RDPEQGKYIVPIVPDEARTFGMEALFRQVGIYSSVGQNYEPVDMDTLLYYKEAKNGQI
                     LEEGITESGSMCSFIAAGTAYANHGINTIPFFIYYSMFGFQRIGDLIWAAADMRCRGF
                     LVGGTAGRTTLAGEGLQHQDGHSHVLALPVPNLLAYDPAFAYEIAIIVQEGIKRMYVD
                     QESIFYYLTV"
BASE COUNT         1387 a         1798 c         2071 g         1344 t
ORIGIN      
        1 gagacgatgc ggctcatggt ggggggcttg acctgctcgg cggcggcgag ttcgcccagg
       61 gttttcgggc ctccgaagac caggacggag agggcggaga ggcgagcggg gccttcgccg
      121 gtttcgacgt cctgcttgcg gacccggcgc agcaggtgga tggcgacgga gtgcaggcgg
      181 tcggcgaggg agagcaggtc actgttgatg gttgatgctt gatggtcgat cgaagaatcg
      241 cgtgacatga gggcttgaca attcctctcc aatatagtta gctatgctaa ctaatttgat
      301 tcaggaggtc aagacatgcc tatggtggaa acggggatcg ggattcgaaa gatcgggcag
      361 attcagctgc gggcgcacga tgtggagcgc gcggcggcat tttacgagaa tgttctgggg
      421 ctgaagctgc tgttcaaggc accgccgaat ctggcgtttt tcgattgcgg cggagtgcgg
      481 ctgatgatcg acaagccgga gaagccggag ttcgatcatc cgggatcgat tctgtatttc
      541 acggtgccgg acatccaggg ggcgcatgcg cggctgaagg agcagggggt gcgtttcgaa
      601 gacgagcctc acatgatcgc gcgcatgccg gattacgatt tgtggatgac gtttttccgc
      661 gattcggaag acaacctgat ggggctgatg agcgaagtgc ggcggtaggt tttcgggcgg
      721 tgtagggctg atcgtgtgag gcctgaggcc tcacacgatc gttcgatgcc cacgcgatcg
      781 ttgcctgcaa aaatcacgcg tcggcgcaaa gcagcagagg aaagaaaact acttcgcccg
      841 attgcgatag agcgacccgt cgacctgctg cgtcatcgca tcgacatcca aggggatctc
      901 caggaaatcc gagacctgct ttgcgacctc tcccgggttg ctcagcacat tgtggtaggg
      961 aacgcgtaaa gccttcacgt agctcttccc gtccagccac gcgtagactt cgttgagatg
     1021 tttctcgaaa gccgcggcaa tgaccgcagg gtcaacccct tccttgtaag tgcctctgcg
     1081 gcgcagcatt tcgtcctgtg acttcagcac ctcgggcagc ggccgctgca tgaaaatgac
     1141 gcgatactca tggccggcag gcagggagag caagagcttg gagatcgcct ttaccacttt
     1201 gccttcggct tcggcaatgc agccgggatc gttcgggagt tgcttgatcc gctcccattc
     1261 caggtatccc ttggggttgt ctacatcggc ccggcgctcc ccgtcggaga gcgcctgcat
     1321 cccgcccgcc accagcatct gcatcatgag agaagtgccg gaacgcggca atcccgaaac
     1381 gattgtaatc atgttctctt tattattatg actcggggag ggcaggacct tggtccaacc
     1441 ggcggaatgt agtttaaccc gcgggttgat acatggccca gggaaggcaa ccttgctgag
     1501 aaacgtctgc aacgggtaga ctcaagggcc gcaaatcagt tttccaaaca ggtctctcaa
     1561 ggattcccat gggtccacgc agaactcttc actccaccgc agtaaacctc gtggtgtccg
     1621 cctatgtttt tctctcggtc agctgtgtgc tggcgcagag ctccgaccca gggtctgcgg
     1681 tcagcgatta cgtcaaggct gagatgcagc ggcagcacat tcccggactg tcgttgctgg
     1741 tcattcataa cggcaagact gttcttgccg agggctttgg tctcgccaac gttgagttgc
     1801 aggttgctgt aaagccggaa acggtctttc agtcgggatc gatgggcaag cagttcacgg
     1861 ccacagcggt catgatgctg gtggaggaag gaaaagttgg gctggatgat ccgctgacca
     1921 agtattttcc cgaagctccg gcggcgtgga aggaagtgac ggtgcgcgag ttgcttagtc
     1981 acaccggcgg ctttggcgac tatcccaagg atttcgatct gcagaaagac gttaaggaag
     2041 aagacgagct gaagactttt gagcagatac cgctggcatt cacgccgggc actaagtggg
     2101 agtacagcaa tctcggctac atgacgctgg gcgtgttgat tcatcgcgtg acgggcgagt
     2161 tttatggaga tttcctgcaa cggcggatct tcaagccgct ggatatgcag accacgcgca
     2221 tcatcagcga ggccgacatt gtccccaatc gggcggccgg ttaccggctg gtgaagggcg
     2281 aactgaagaa tcaggaatgg gtagctccga ccatcaatac cacagcggat ggcagtctgt
     2341 atttttcgat tctcgatctg gccaagtggg acgctgcgct ctacacggag aaactgctga
     2401 aacggtcgag cctggagctg atgtggactc cggtgaagct gaaggacgga aaggcgaata
     2461 agggcgatta cgggtttggc tgggtcattg tgcagcggca cgggcaccga tgcattacgc
     2521 acgatggtgc gtggcagggc ttcgagacca cgatcgaccg ctacgtggac gatcacctca
     2581 ccgtggttgc gctcgacaac ctgtcagagt cgcagccgtc gaagatcgcc gagcacgtgg
     2641 cggagttgta tttgggggaa aagtagcagc tcggaaagcg ttcgtctcat agggaacggt
     2701 ggtcgcttac ggcaaagccc gtgattcttg ctcgctaccc ggcacggctg aagtcttcgc
     2761 ttccgaaact gcacgctgac agctcttcag tttgacaggg acattcggag ctgcgtctga
     2821 ggcaggattg cagcggctat ccttgaactg cgggtctcat tcggctatgc aaagaggagc
     2881 caggaagaga tggagccaaa ccgtcttagc cgcaggagtt ttcttgcgtt gactactatg
     2941 gcggccgccg gtgttactct gaagccagcg tttggagaat cgcaagacct tgcgtactta
     3001 acgctgaaag aagcgtccgg actggttcgc cgcaagaccg tttcgcccgt cgagcttacc
     3061 gcgacttgcc tcaagcgcat tgaagcttac aaccccgcgc tgaatgcctt catcaccgtg
     3121 acgaagggcc aggcaatgga aaccgcacgt gccatggagg cggaacggca gcaagggaaa
     3181 tggcgagggg cgctccatgg catccccatt gcgctgaaag acaacatcga cactgcgggt
     3241 gttcgcacca cagctgccag cgaattgttc aaggagaggg ttccatccaa agatgcggaa
     3301 gtggttcgac ggctgaagga ggcgggggcg gtgctcctgg gcaagacgaa catgcacgaa
     3361 tttgcgttag gcggaacgtc ggcggtcagt tacttcgggc cggtgcacaa cccctgggcg
     3421 ctggaccgga ttcctggcgg atcgtcgggc ggatcagccg ccgccacagc cgctggtttg
     3481 tgcttcggat cgttgggaac cgacaccgcc ggatcagtgc gaattcccgc cagctactgt
     3541 gggattgtcg ggttcaggcc cacatacggc cgggtgagca gtcgcggaac ggtaccggac
     3601 tcatggaccc tggaccaagt tggccctctg tgcaggactg tcgaagacgc ggccctgatg
     3661 cttggcacga tcgctgccta tgacgaattc gatcccgctt cggttgacgt gccggtggcc
     3721 gactatagcc tcgcattcca gatgcaggta tcgcagctcc gtttggggat tccccgggtt
     3781 ccctttttcg agggtctcga cccggagatc gagaaggcta cggcagccgc aatcgaggtg
     3841 atgggaaaac tcgcggcaga agtgcgggac ctcacctcct cgatcgtggc agcgcacctt
     3901 atggaaattt gggtcaaggt ggccggggcc gagtcttaca cgtatcactc gcactggctt
     3961 gccgagtcgc ccgagaaata tcaggctgcg acgcgtggcc ggctgctcgc gcccaacaac
     4021 gcgggcgtgc agggcgagaa ctcgacccag atgaaggcat cgacctacct ggaggcgcgt
     4081 cgtcaactcg atgtgttgcg ccgggagatc cgcaaagtat ttgccaatgt cgacctcctg
     4141 atcctgccta ccatgcttgc cccgccggta accatcgcgc agggcggaga cccgaccatc
     4201 gtaaatccgc gcaacgccgc ttcgttttcc gttctggggc tgcccgcgct ttccctcccg
     4261 tccggtttta ctacaggcgg tctgccgata ggcctccaaa ttgtcggggc tccgttcgcc
     4321 gagtcaaccg ttctggcgct ggcacatgcc tacgagcgcg agacggaatg gcacaaaaga
     4381 catccgaaag tcgatccggc ttaaggctcg ccaaattaga cctggatggc tagcaactcg
     4441 gaaactcact taccgtcctc cagcaaaaat tcctacgatt caatatgatg aaccattacg
     4501 cttttagcga cggagaaacc tgacgtggct catacggaaa ccgatggctt agaggtgttg
     4561 gaaaccaggg agtgggtcga ctcgctcgat tatgtactgg aaaaaggcgg ccccaaccgc
     4621 gccggacgcc tgttgcagca gttggcgctg cacgcgcggc gcgaagcggg gatcaatctg
     4681 ccgttctcgg cgaccacgcc gtatcagaat acaatcccgt cccgccagca ggcgccattt
     4741 cccggcagcc aggaaatgga gcggcgcatc aagagcctgg tgcgctggaa cgcgctggcc
     4801 atggtggttc gcgcgaacaa gattcaggaa ggcatcggcg gacatatttc cactttcgct
     4861 tcggcggcga cgctgtacga agttgcattc aatcattttc tgcgcgcaca aaccgaatcg
     4921 ggcgatcgcg acgtcgtgta tttccaggga cacgctgcgc cgggaatgta ttcgcgcgcc
     4981 tatcttgaag ggcgcattcc gaaagagaag ctgcagaatt ttcgccgcga gttaaagccg
     5041 ggcggggggc tgagttcgta tccccatccc tggctgatgc cggatttctg ggagttcccc
     5101 acggtctcga tggggctggg gccgattcag gcgatctatc atgcgcgttt catcaagtat
     5161 ctcgagaatc gcggcctcaa gcagtcgacc ggtggcaaag tctgggcctt cctgggcgac
     5221 ggcgaaatgg acgagccgga gtcgctgggc tcgatcacgc tggcttcgcg cgaaaggcta
     5281 gacaatttgg ttttcgtcgt gaactgcaac ctgcagcggc tcgacggacc ggttcgcggc
     5341 aacggcaaaa tcattcagga actcgaggca attttccgcg gcgcgggatg gaacgtcatt
     5401 aaagttatct ggggcacgga ctgggacaac ttgatccaga acgatcgtga cggtctgttg
     5461 gtgaagcgcc tgggagaagt gagcgacggg caatatcaga aatactttgt cgagagtgga
     5521 gcatacttcc ggcacaactt attcggtacc gatccgcggc tgctgaagat ggttgagcac
     5581 ctgtcagatg agcagctggc gcgcatgcgg ctgggcggcc acgatccgat caaggtgcac
     5641 gcggcgtaca aggcggccgt cgagcacaca ggatcgccga cgattattct ggcaaagacc
     5701 atcaaaggct acggcctagg cgaggcgggc gaaggcaaga acattaccca tcagcagaag
     5761 aagctgaatg acgacgagct gcagatgttc cgctcgcgct tcggcatccc catccctgac
     5821 gacgagctgc acgatgcgcc cttctatcgt ccggctgacg atagcccgga gatcaagtat
     5881 atggaggcgc gccgccagca actcggggga tacatgccga cgcgcaaggt ccgggccacg
     5941 ccgctgaagc catttcctga ttcgcatttc gaggagttcc acaaaggcac cgaggggcgc
     6001 gaagcttcga ccacgatggt tttcgtgcgg ctgctttcca agttgctgcg cgatccggag
     6061 caggggaaat acatcgtgcc tatcgttccc gacgaagcac gcacgttcgg catggaagct
     6121 ctcttccgcc aggtgggaat ttattcgagc gtcggccaga actatgaacc cgtcgacatg
     6181 gacacgctgc tctactacaa agaagcgaag aacgggcaga ttctggaaga aggaatcacc
     6241 gagtcaggat cgatgtgctc gttcatcgcg gctggaacgg cgtatgcgaa ccacggaatc
     6301 aacacgattc ccttctttat ttattattcg atgttcggct tccagcggat tggcgacctg
     6361 atctgggcgg cagctgacat gcgttgccgc ggcttcctgg tcgggggaac ggccggtcgt
     6421 accaccctcg cgggcgaggg cctgcagcac caggatggtc acagccacgt gctggcgctg
     6481 ccggtgccga atctgctggc ttacgatccg gcgtttgcat acgagatcgc aatcatcgtg
     6541 caggagggca ttaagcggat gtacgtggat caggaatcga ttttctacta cctgactgtc
//
LOCUS       DASPUL010000486         5404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_924504, whole genome shotgun sequence.
ACCESSION   DASPUL010000486 DASPUL010000000
VERSION     DASPUL010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924504"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..537
                     /locus_tag="VEJ00_11540"
     CDS             124..537
                     /locus_tag="VEJ00_11540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXX01842.1"
                     /translation="MKTLTEYLKFHTRTHRAYVHITPQVEEIVRKSEVKEGMVLVSAM
                     HITAGIYVNDNESGLIEDIDQWLEHLAPFRKDYKHHETGEDNGDSHLKALLIHHEVIV
                     PITAGKLDLGTWQRIFYAEFDGQRDKRVIVKVMGQ"
     gene            complement(638..1258)
                     /locus_tag="VEJ00_11545"
     CDS             complement(638..1258)
                     /locus_tag="VEJ00_11545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX01843.1"
                     /translation="MKKTVSGKRQLRRCLKALEGEGRVTCAYLRSWPQIQAALPAFVD
                     AHVARFQAKQRVSFLSAPERRVLLEELARRLSSSGVMTFSTLLVDDRPIAWSFGFQFH
                     ESWFLYQTTFDASREENSPGYCLLGKILIEACDTGTFRRVDLGLGAEGYKEWFANSTR
                     QTLYATLTSSYLRHLRTIARDRIAAQVQRYPKLEAAIRQTQARLGL"
     gene            complement(1925..2152)
                     /locus_tag="VEJ00_11550"
     CDS             complement(1925..2152)
                     /locus_tag="VEJ00_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01844.1"
                     /translation="MTDYSKMSYEALSQLANNDSIADKMSDEEYESLSIELWRKFGVE
                     RALVEHYTAAPRSPREKNPVPGALRDGWVLG"
     gene            2887..5256
                     /locus_tag="VEJ00_11555"
     CDS             2887..5256
                     /locus_tag="VEJ00_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01845.1"
                     /translation="MDTASAANHYVYKVYISGTGTPEAVPVTGGSCAPGAASGTITVT
                     TANAHAAGYTVGSASSGIQEAWNDAWVNDNGAAPNPNSQTAPYVKLMADTKYNVYAAV
                     YLRGRGGILDGAGALIACSTRDRCIYVGTTAGIPSVNHHKLYNLSGTSTINVDGVRVS
                     SVAAAGGTYTVTTASAHPFVAGDTVDCEYHTTMADQHWSALVQSVPNSTSFTASLGRA
                     TFSRASGFGFCNLLNAFLEDNSDHVALQDINLFLSNNSGMGHFSYGIVNDNDQQFIIE
                     RAANRSSGILNATANWPIGAFFYQRTDQGNAGIMYVHSSEITGVNCATGGGNGFVMSD
                     TVCQGFPVYGVRYFGGLQPATFQNVYQESSGGTIDPLYGAAAQAGLLVQGGFGTKILG
                     TFPLSGYSPAFAAGGGQAAERTYFVVPHSSTQGYGPMLFIGFAEPKSRTSIKVSWPAI
                     EFQNNVGQSLGTVTWDVLVTTAGAAGPPTGTGNFAIATGISGSCGTNGMCSFTDTQAA
                     PSSYTVPAQQFTPVFWFWPVSLVINGTTVFADAVGGSPSAVASQGATAVSIVAEQCKS
                     FGAARQRSPIWVSCLTSDGNGGSGTIATVLQEQDGANNGPAADSKGRLNFGKPIRNTP
                     NDLITLQDSNFIKTTSTAGERPSNDAGDMALGLDQPGGLAERAQNSISQYIDALPRGT
                     NYLERLTAAAKTFKVPIATPAAIIQLSTSPIAAHTCRVQGTELKGMTTSSVIKWSYAG
                     SAIRVAGYGTGELQISTFSTPGTGNIMVCNTSERTITPGAITVNIREEL"
BASE COUNT         1219 a         1454 c         1656 g         1075 t
ORIGIN      
        1 cccacgatgg agaaagatcg agtcgtgtat gccgacctcg cccgcattgc cgaagtaatt
       61 cgcagcggga agttggctga ggtcctgcga tagtcccaga attgcagtat cggaactctt
      121 cgaatgaaaa cccttaccga atatctgaag tttcacacca ggacgcatcg ggcttacgtg
      181 catatcacgc cacaggtgga agagattgtc cgcaagagcg aagtcaaaga aggaatggtg
      241 ctggtttcag ccatgcacat tacggcaggc atatatgtga acgacaacga aagcggattg
      301 atcgaggata tcgatcagtg gctggagcat ctggctccgt ttcgcaagga ctacaagcat
      361 catgagaccg gagaagacaa cggcgactct cacctgaaag ctctactgat ccatcatgag
      421 gtcatcgtgc cgatcacggc cggcaagctg gatctgggga cctggcagcg aatcttctac
      481 gccgagttcg acgggcaacg ggacaagcgg gtcattgtga aggtcatggg acaataacaa
      541 tggagcgcca ttgaacctct cctttgatat caattctgca atgaccactt ctcacggaaa
      601 acagtaccgt gatcaaacga cgtattcgga ggaatcctca cagtcccaat ctggcttgcg
      661 tctgtcggat cgcggcttcc agtttcgggt agcgttgcac ctgcgctgca atgcgatccc
      721 tcgcgatcgt acgcaggtgg cgcaggtaag agcttgtcaa ggtagcatac agggtctggc
      781 gagtgctgtt cgcgaaccat tccttgtaac cctcggctcc taggcccaag tccacgcgcc
      841 tgaacgtgcc tgtgtcgcac gcttcgatca agattttccc gagcaggcag tagcctggag
      901 aattctcttc gcggctggca tcgaaggtgg tctgatacaa gaaccaactc tcgtggaatt
      961 gaaaaccgaa gctccaggcg atcggccggt cgtcgactag cagcgtactg aaagtcataa
     1021 ccccggaact ggatagcctc cgtgcaagtt cctccagcag gacccggcgc tcgggggcgg
     1081 agagaaagct gacgcgctgc ttagcctgaa aacgcgccac atgggcgtcg acaaaagccg
     1141 gcagcgcagc ctggatctgt ggccatgatc gaagataagc acacgttacc cgtccctcgc
     1201 cctccaacgc tttcagacac cggcggagtt gtctcttccc cgaaacggtt ttcttcaatt
     1261 cctgtcgttg cgcggtggaa cccagctcta cctgggggca caagtatgct gggcgaatgt
     1321 acacatgaaa accatgcctc ttcgctgccg cccgcagggc ggcaggggtc gcagagtctg
     1381 ccggcaaatt ggcaagcacc aaccttccca gcctccgttg ctgaagctcg gcgaagaccc
     1441 cctcaaccaa ctctgcccgt cgctggggat gactgagaaa ctcacagtaa tctgcggtcg
     1501 tgccagcgag aaagctcaca atttgtgtgc tggggtctgt gcacagagat gccactccca
     1561 ctaggttctc accgtcgtag ccaagaaaca gcagaggctt cacagaggct tggtaagcag
     1621 actggacggc gagcgcccac tcgcatgtat agaagacctg agctctttcc atctgcagga
     1681 ctatgtcatt ccactgtccc ttcagagaag aatcttccgg aacttgtctc agcacagcta
     1741 tgcgcaaact tattgccccc ttgggctggt ggtggaggaa tatggtagtt gattcgtccg
     1801 taactcgcta ctcgacaacg atgcgagggg acatgcaaca ggtgttccgg agactctctt
     1861 atgtattttc gacgacgaag cggaaagcag gagggtcagc gtccgaacgt tgagattgcc
     1921 agaactagcc caggacccag ccgtctctaa gagcgcctgg gaccggattc ttctcgcggg
     1981 gagaccgggg tgcagcggta tagtgctcta cgagcgctct ctcgacaccg aactttctcc
     2041 aaagttcaat ggataaggat tcgtattcct catctgacat cttgtcagca atggagtcgt
     2101 tgttggcgag ctggctaagc gcttcatagc tcatttttga atagtcggtc atcgcggttc
     2161 tcctttcttg gcactcttga gtgcacatgg agaaagctcc ggtggagact ccagttcttc
     2221 acctctgtct tctaagggag atttacagtg cttacgccaa gcacgtcaac ggaaactcgg
     2281 caggtgatga tgactagaat ggttctcgcc catcggtgcg ggccaagtcg tttggtgctc
     2341 gcggtgcggt ggtgaagcgt agcgatgacc gttccaaagt cagttggcga aattcagata
     2401 ggatgagaag gcgatcgcca cctgttttcc tcgggatttc gtgcatgtga ccttggcccc
     2461 agatcattat gattcgtttt cgtgacctcc caagccccgc ggagcgcagc tcggatcaac
     2521 aagacctgac gatttctgtc gcgaagcaag cgacgtactt tgacgatggc ttgatagaag
     2581 ctcggcctca cggctaccct tttcagggca cgccaaacga cttggtaccc cgtgcgacgc
     2641 gaacaatctg tcaggagatg ccttgtgtca aaaacaggac tcttgctgtc gcttttgttt
     2701 actctttgct tgacgcagac cccagtatct agcgacttgc aagaaccagg gactggcaaa
     2761 ctaccccggc cgagcgaagc aaccatgcgg tacgtcacgc ctgcttggaa ctggacacag
     2821 cctctgaggg acgagctctc aatttcagga aagaaaacgc tccacttaag cccatgccct
     2881 cgaggaatgg acacggcgtc agccgccaat cactacgtgt ataaggtcta catttccggg
     2941 acggggacgc cggaggcggt cccggtgact ggcggaagtt gtgcgccggg agcggccagc
     3001 ggaacgatta cagtgaccac tgcgaacgct cacgcggcgg gttatacggt gggttcggcg
     3061 agttcgggga ttcaggaagc gtggaacgac gcgtgggtga acgacaacgg cgcggcgccg
     3121 aacccgaact cgcaaaccgc tccgtacgtg aagctgatgg cggacacgaa gtacaacgtc
     3181 tatgcagccg tctacctgag agggcggggc gggattctgg acggcgccgg ggcgttgatc
     3241 gcctgctcaa cgcgggaccg gtgtatttat gtgggaacga cggccgggat tccgtcggtg
     3301 aatcatcaca agctgtataa cctgtcgggg acgtccacca taaacgtgga tggagtgcgg
     3361 gtgtcaagcg ttgcagcggc gggcgggact tatacggtca cgacggcgag cgcgcatccg
     3421 ttcgtcgcgg gcgatacggt cgattgcgaa tatcacacca cgatggcgga ccagcactgg
     3481 tctgcgctgg tgcagagcgt gccgaactcg accagcttca cggcgagcct aggaagggcg
     3541 actttttcaa gggcgtccgg gttcgggttc tgcaatcttc tgaacgcgtt cctggaggac
     3601 aactccgatc acgtggccct gcaggacatc aaccttttcc tgagcaacaa ttccggaatg
     3661 gggcacttca gctacggcat cgtcaacgac aatgaccaac agttcatcat cgaacgggcg
     3721 gcaaaccgtt cgagcgggat cttgaatgcg accgcgaact ggccgatcgg ggcgttcttt
     3781 taccagcgca ccgatcaagg aaatgccggg atcatgtacg tgcacagctc ggagatcacg
     3841 ggagtgaatt gcgcgaccgg cggcggcaac gggtttgtga tgagcgatac ggtgtgccag
     3901 ggatttccag tgtacggggt gcggtacttc ggcgggctgc agccggcgac gtttcagaac
     3961 gtgtatcagg agtcgagcgg aggaacgatc gacccgctgt acggagccgc ggcacaggca
     4021 ggattactgg tacagggggg attcggcacg aagatcctgg gaacgtttcc gctgagcgga
     4081 tactctccgg cattcgctgc gggaggagga caggcggctg aacgaaccta ttttgtcgtt
     4141 ccgcacagct cgacgcaagg atacgggccc atgctgttca tcggatttgc ggagccgaag
     4201 agtcggacca gcataaaggt gtcgtggcct gcgatcgagt ttcagaacaa cgtggggcaa
     4261 agcctgggca cggtgacgtg ggatgtgctg gtgacgacgg cgggagcggc gggccctccg
     4321 acaggcacgg ggaacttcgc gattgcgacg ggaatctcgg gatcgtgcgg gacgaacggg
     4381 atgtgctcgt tcacggacac gcaggcagca ccgagttcgt atacggtgcc ggcacaacag
     4441 ttcactccag tgttctggtt ctggccggtc agcctggtca tcaacgggac gactgtgttt
     4501 gccgatgccg tcggggggag cccgtcggcg gtggcgagcc agggagcaac ggcggtctcg
     4561 attgtggccg agcaatgtaa gtcgttcggg gcggcgcggc agaggagtcc gatctgggtg
     4621 agttgcctga ccagcgacgg caacgggggc tccgggacga tcgcaaccgt gctacaggag
     4681 caggacggcg ccaacaacgg gccggctgcc gacagcaagg ggcggctgaa ttttggcaag
     4741 ccgatcagga atacgccgaa cgatctgatc acgctgcagg actcgaattt tatcaagacg
     4801 acctcgacag caggagagcg tccatcgaac gatgcggggg acatggcgct ggggctggat
     4861 cagccgggag gactggcgga acgggcgcag aattcgattt cgcaatacat cgatgcgctg
     4921 ccccgtggaa cgaactatct ggagcggctg acggcggcgg cgaagacgtt caaggttccc
     4981 atcgcgaccc ctgctgcaat catacagctg tccacttcac cgattgctgc tcatacatgc
     5041 agggtacagg gcacggaact caaggggatg acgacaagtt ccgtcattaa gtggagttac
     5101 gccggctcag caattagggt agcaggttat ggaacgggag aactgcagat ctcgacattc
     5161 tcgacaccag gtaccggcaa tattatggtt tgcaacacca gcgagaggac tattactcca
     5221 ggggcgatca ctgtgaacat tcgggaggaa ctgtaggcaa gagaaagggt gtttgctccg
     5281 attgctggaa cgcaggcaaa acgccaagca gtttgaggtc tccggcactg aggacgaaac
     5341 ccaccggtca aaaaacaggc ggagcgacat cgtatccttg aaggctcgga ggacgggatc
     5401 ccgc
//
LOCUS       DASPUL010000487         5273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_232821, whole genome shotgun sequence.
ACCESSION   DASPUL010000487 DASPUL010000000
VERSION     DASPUL010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232821"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VEJ00_11560"
     CDS             <1..634
                     /locus_tag="VEJ00_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01846.1"
                     /translation="APTENGQPAALAAALSLGERFGRIYDNPYNAPAVQSVKLDFDLV
                     HERRWARLESARTDVTEVRPGDEIMVETVLAPYRGERSVRQIPVKIPTSASKGILRIF
                     VSDGETLDRMSRINPVFGRKLDLASTIAMLNKEHANNRVYVSLLEADPEARVADKVMP
                     TLPLSVMNVMDGMRGSQEMTVSGESNVDETATPPLDYVVSGAQLLTVTVK"
     gene            complement(742..1221)
                     /gene="bfr"
                     /locus_tag="VEJ00_11565"
     CDS             complement(742..1221)
                     /gene="bfr"
                     /locus_tag="VEJ00_11565"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HXX01847.1"
                     /translation="MKGNPKVLAELNKALREELTAINQYFLHAEMCENWGYHKLADFI
                     KKQSIGEMKHAEVLIERILFLDGSPSMMPLELKVGGTVKSMLESDLALEVGAVKQYND
                     AVAIASKEGDNGSRDLLVTLLKDEEEHVDFLEAQLHMINELGYERYLTMQMGEDEEE"
     gene            complement(1327..2055)
                     /locus_tag="VEJ00_11570"
     CDS             complement(1327..2055)
                     /locus_tag="VEJ00_11570"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HXX01848.1"
                     /translation="MFGIALLTVLTLFIVGSITLVAAWRLLYPHASLNEVAQKQALGI
                     VGQALSYVIICLYMIMLVEGKYHVRFWRAIGWNWPATGLKITGFAFLGVCTVLLDIVA
                     SRVLPMPKSTPFDQFFTNPRDAYPLAAFAITLGPLWEELFFRGFLYPALARRTGVATA
                     VVLTSLPFGLIHYPQYKSWAAVLVIVMVGVVLTGVRAVTKSVGASLVVHVGYNTTLMM
                     LTFAATGGFRHMEKATALVTVAIR"
     gene            complement(2308..5115)
                     /gene="uvrA"
                     /locus_tag="VEJ00_11575"
     CDS             complement(2308..5115)
                     /gene="uvrA"
                     /locus_tag="VEJ00_11575"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001503.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXX01849.1"
                     /translation="MAITKISVRGARQHNLKNIDVEIPRNTLTVVTGLSGSGKSSLAF
                     DTIYAEGQRRYVETLSAYARQFLDQMERPDVDAIDGLSPSISIEQKTTSRSPRSTVGT
                     ITEIYDYLRLLFASIGVPHCPRCGRAISRQSADQIVQRVMALTPEDRVMVMAPIVRGR
                     KGEFKKEMETLVKHGYTRARIDGELANLDDDIQLDKRKNHTIEVVIDRLLVKPGIEHR
                     LEMSVGLAMKLAGGLVQVAVVGGEEQLYSEKLACPDCGINVPQLEPRSFSFNSLYGAC
                     PECHGLGSKYDFDPAKVISDWSKPLLDGGLGPGSASQNLIHQLQIVSAAYGIDLSTPF
                     EKLPEKIQDFLLNGEQGRGGRTGFHGIFGYLKQNLEESTSEGYRDWLMDHMSATECPV
                     CHGKRLRPESLAVKVNGMSIAEFTAMPVSKALSVAQGIKLAGREAAIAGRVMHEVVER
                     LQFLNAVGLGYISLDRSAATLSGGEGQRIRLATQIGSRLRGVLYVLDEPSIGLHHRDN
                     GRLLNALENLRDLGNTVLVVEHDEETIRRADYVVDLGPGAGRHGGSLVAHGTPEEIMR
                     EPNSLTGAYISGRTQIEMRPERRQKNGAALTILGAKENNLKNLDVTFPLGVMTVVTGV
                     SGSGKSTLVNDILYRALARQLYRSREKAGEHKAISGAENIDKVIRIDQSPIGRTPRSN
                     PATYTGVFTAIRDLYAMLPESRERGYKAGRFSFNVTGGRCEACQGEGQRRIEMNFLPD
                     VYVLCEVCGGRRYNSETLAVKYNGNSIADLLEMPVSDALPILENIPQVKQKLQTLVDV
                     GLGYIHLGQSAVTLSGGEAQRIKLARELSKRQTGKTLYLLDEPTTGLHFDDVKKLLDV
                     LHRLTDLGNSIIIIEHNLDVIRNADWIIDLGPEGGEDGGRIVAQGTPEQVARVKKSYT
                     GQVLAGYFGRAVVSAQLPVVS"
BASE COUNT         1062 a         1665 c         1488 g         1058 t
ORIGIN      
        1 cgctcccacc gaaaacggac agcccgccgc cctggccgct gccttgtcgc tgggcgagcg
       61 ttttggccgg atctatgaca atccttacaa cgctccggca gtgcagagtg tgaagctcga
      121 cttcgacttg gttcacgagc gccgctgggc gcgcctggag agcgctcgca ccgacgtgac
      181 ggaggtccgc cccggcgacg agattatggt cgaaaccgtc ctggctccct accgcggcga
      241 acgcagcgtg cgccaaattc cggtgaagat tccgacgtcc gccagcaagg ggatcctgcg
      301 catctttgtg agtgatggcg aaaccctcga ccgcatgagc cgaatcaacc ccgtgtttgg
      361 ccgcaagctc gacctggctt cgaccattgc gatgctgaac aaggagcacg ccaacaaccg
      421 cgtctacgtt tcgcttctcg aagccgaccc tgaagcccgc gtcgccgaca aagtcatgcc
      481 aacattgccg ctcagtgtca tgaatgtgat ggacggcatg cgcggcagcc aggagatgac
      541 cgtcagcggc gaatcgaatg tcgacgagac ggcaacgccc ccactcgatt atgtggtcag
      601 cggcgcgcag ttgttgacag tgacggtgaa gtaagcgacg gcgcggaact tgtgtggcgc
      661 gggcactcct gcccgcgaac cataaccaga aaaacaggtg ctccatttct cgcgttcttt
      721 gcgagaagtg gggctttcta actactcttc ttcatcttca cccatctgca tggtcaaata
      781 ccgctcgtac ccgagctcgt tgatcatatg caactgggct tccagaaagt cgacgtgctc
      841 ttcttcgtct ttcagcaggg taaccagcag gtcgcgcgag ccgttgtcgc cttctttgct
      901 cgcgatggcg acggcgtcgt tgtactgctt caccgccccg acctcgagcg ccaagtcgct
      961 ttcgagcatc gacttgactg tgccgccgac ctttaattcg agcggcatca tggacgggct
     1021 gccatcaaga aacaggattc gctcgatgag cacttctgcg tgcttcatct cgccgatgga
     1081 ttgtttttta atgaagtcgg cgagcttgtg atacccccag ttctcgcaca tttcggcgtg
     1141 cagaaaatac tggttgatag cggtcagctc ttcgcgcagg gctttattca attcagcaag
     1201 aacttttgga tttcctttca tagcgcccct gagtatgcca gaaaaccgct aactgggtaa
     1261 acagcctgcg caacggaata ttcggatttg cggaccagat caggaaagtc gccgccccgg
     1321 cctcggtcaa cggatggcga cggtcacaag ggccgttgcc ttttccatat gccggaagcc
     1381 gccggtggcc gcgaaggtca gcatcatcag cgtggtgttg tagcccacat gcaccaccag
     1441 acttgcccct acggacttgg tgaccgcgcg cacgcccgtt agcacgactc caaccatgac
     1501 aatgaccagc acggccgccc aggacttgta ttgcggatag tggatgaggc caaacggtag
     1561 agacgtaagg acgaccgccg tggcgacccc cgtccgccgg gccagagcgg gatagagaaa
     1621 accgcgaaag aacagttcct cccacagcgg ccccagagtg atggcgaatg ccgccagcgg
     1681 gtacgcgtcc cgcggattcg tgaagaactg gtcgaagggc gtgctcttgg gcatgggcag
     1741 aacgcggctg gccacgatat cgagcagaac cgtacacact ccgagaaacg cgaatcccgt
     1801 gatcttcaaa ccggtcgcag gccagttcca gccgatcgct cgccagaagc gcacatggta
     1861 cttcccctcc accagcatga tcatgtagag gcagatgatg acataggaca aagcctggcc
     1921 cacgatcccc aacgcctgct tctgcgcgac ctcgttcaag ctggcgtgtg gataaagcag
     1981 ccgccacgcg gccaccagag tgatcgatcc gacgatgaac aaggtaagca ccgtcagcaa
     2041 cgcgatcccg aacacgtccc agccgctcca gacggggttt tcggcagatg tggaggtcgc
     2101 cggggcaggg taagccggcg catagatccc cggcccgcca gcgtcaggag ggaacgacgc
     2161 tggcgcgaac ggatcagccg ccgtcggtgc gggttcctgc gattcggggc caaccggagt
     2221 tggttcgaac tgcggattgt cagaggatga cagaggacag gtcccttcta gctgcgctca
     2281 attggcaatc ggaacttggc aatcgcacta actgaccacc ggcaactgag cactgaccac
     2341 tgctctccca aaataccccg ccagcacctg ccccgtatac gacttcttca ctctggctac
     2401 ctgctcgggc gtgccctggg ccacgatccg tccgccgtct tcgccgccct cggggccgag
     2461 atcgatgatc cagtcggcat tgcggatcac atccagattg tgctcgatga tgatgatcga
     2521 gttgcccaga tcggtcaaac ggtgcagcac gtcgagcaac ttcttgacgt cgtcgaagtg
     2581 taggccggtg gtgggctcgt cgagcagata tagcgtcttg cctgtctggc gcttgctcag
     2641 ctcacgggcc agcttgatgc gctgcgcttc gccgccggag agcgtcaccg ccgattgccc
     2701 gagatgaata tatcccaggc ccacatcgac cagcgtctgc agcttctgct tcacctgggg
     2761 aatgttttcg agaatcggca gcgcgtcgga aactggcatt tccagcaaat cggcgatcga
     2821 atttccgttg tatttcaccg ccagtgtttc tgagttgtag cggcggccgc cgcagacctc
     2881 gcacagcaca tacacatcag gcagaaaatt catctcgatg cgccgctggc cttcgccctg
     2941 gcaggcctcg cagcgtccgc cggtaacgtt gaacgaaaat cgtcccgctt tatagccgcg
     3001 ttcgcgcgac tcgggcagca tagcgtacag atcgcgaatc gccgtgaaaa cccccgtgta
     3061 ggtcgcggga ttagagcgcg gcgtgcggcc gatcggagac tgatcgatgc ggatgacctt
     3121 gtcgatattc tcggcgcccg aaatcgcctt atgttcgccg gccttctcgc gcgagcggta
     3181 aagctgccgc gccagcgcgc gatacaaaat gtcattcacc agcgtcgact tgcccgaacc
     3241 ggaaacaccg gtcacgaccg tcatcacacc cagcgggaac gtgacgtcga gattcttgag
     3301 attgttctct ttcgcgccca gaatcgtcag cgccgcacca tttttctgcc gccgctccgg
     3361 ccgcatttcg atctgcgtcc ggcccgaaat gtaagcgccg gtcagcgaat tcggctcgcg
     3421 catgatctct tccggagtcc cgtgtgcgac caggcttccg ccgtgccgcc ccgcccccgg
     3481 cccgaggtcc accacgtagt cagctcgccg gatcgtttct tcatcgtgct cgacgacgag
     3541 cacggtgttg cccaggtcgc gcaggttctc gagcgcgttc agcagccgtc cgttgtcgcg
     3601 atgatgcagc ccaatcgaag gctcatccag cacatacagc acgccacgca accttgaccc
     3661 aatctgtgtc gccagccgaa tgcgctggcc ctcgcctccc gacagagtcg ccgccgaccg
     3721 gtcgagcgag atgtagccca gaccgactgc attcaggaac tgcagccgct caaccacctc
     3781 gtgcatgacg cgcccggcaa tcgccgcctc ccgcccggcc agcttgattc cctgcgcgac
     3841 gctcaaagcc ttagacaccg gcatggccgt gaactcggca atcgacatcc cgttgacttt
     3901 gacggccaga ctctcgggcc gcaggcgttt cccgtggcac acagggcatt ctgtcgccga
     3961 catgtggtcc atcagccagt cgcggtagcc ctccgaggtc gactcttcga gattctgttt
     4021 cagatatccg aagatcccat gaaagccagt gcgcccgccg cggccctgct ctccgttcag
     4081 cagaaagtcc tgaatttttt ccggcagctt ttcaaacggc gtgctcaaat cgatgccata
     4141 cgccgccgac acgatttgca actgatgaat caggttctgc gatgccgatc ccggccccag
     4201 tccgccgtcg agcagcggct ttgaccagtc actaatcact ttcgccgggt caaagtcgta
     4261 cttgctgccc aggccgtggc actccggaca cgcaccatac aggctgttaa acgagaatga
     4321 ccgcggctca agttgcggca cattgatacc gcaatccgga caagccagct tttcggagta
     4381 cagctgctcc tcacctccga cgaccgcgac ctgcaccagc ccgcccgcga gcttcatcgc
     4441 cagcccgacc gacatttcca ggcgatgctc gatccccggt ttgaccagca gccggtcaat
     4501 gacgacttca atcgtgtggt tcttgcgctt gtcgagctga atgtcgtcgt ccagattcgc
     4561 cagttcgccg tcgatgcggg cccgggtgta gccgtgcttg accagcgttt ccatctcttt
     4621 cttgaattcg ccctttcgcc cccggacgat tggcgccatc accatgaccc ggtcttccgg
     4681 cgtcagcgcc atgacacgct ggacgatttg atcggccgat tgccggctga tggcgcggcc
     4741 acacctgggg caatggggca ctccgatcga ggcgaacaac agacgaagat aatcgtagat
     4801 ttcggtaatc gttccgacgg tggaacgcgg gctgcggctg gtggttttct gctcgatcga
     4861 aatagatggg ctaagtccat caattgcatc gacatccggg cgctccatct ggtcgaggaa
     4921 ctgccgcgca taagccgaga gcgtctctac gtatcggcgc tgaccttcgg cgtaaatcgt
     4981 atcgaatgcc agtgaggact tgcccgatcc gctcagtccg gtaacaacag tgagggtgtt
     5041 gcgaggaatc tcaacatcga tgtttttcag attgtgctgc cgggcgcccc ggacggaaat
     5101 cttggtaatc gccattatcg taccggggtt atatcccctt cgtaacgtcg acaggcccac
     5161 agaattgttc gaaagtagga atgcacagct ctactccgta tcttagtcgt caacagcgca
     5221 gagcgtcaat gcgggtagat tgaagacgca cggatttaga tggtgctctg agc
//
LOCUS       DASPUL010000488         5395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_769816, whole genome shotgun sequence.
ACCESSION   DASPUL010000488 DASPUL010000000
VERSION     DASPUL010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769816"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VEJ00_11580"
     CDS             complement(<1..443)
                     /locus_tag="VEJ00_11580"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXX01850.1"
                     /translation="MRRNSMRLSGLIVALFLATVVLGHPTYAQDRKAINLAPAKGLPF
                     SDGVLAGNTLYIAGQEGTDDAGKLAQGGIAPETKTALDNVERVLKAAGFELKDVVSVT
                     VYLADIHDFGEMNKVYKSAFPDPKPVRATIQAAALVNDARVEISA"
     gene            548..1333
                     /locus_tag="VEJ00_11585"
     CDS             548..1333
                     /locus_tag="VEJ00_11585"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HXX01851.1"
                     /translation="MNNSHVWARCRGGIVMIAALGAVFAAVSEVHPLRAQSPAGAATP
                     TSDSDLIAQFRRVEVASVSDALEQITGKRMYMTHRMQPIFTTKFAGFARTVQLKKDEG
                     NKDPDALTGMLEAIDQGSTDSVYVMAVEDGEDIAGMGGLMGTAMAARGYAGAVIDGGV
                     RDVAYLRKIGFPVFATGIVPSTSVHHYRFAGAQIPLVCNGVPVNPGDIVVADSDGVAV
                     VPRAHAQAVLTLAQQMDFKEHSMYAVIEQLKSIVAAVQKFGRI"
     gene            1412..2263
                     /locus_tag="VEJ00_11590"
     CDS             1412..2263
                     /locus_tag="VEJ00_11590"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXX01852.1"
                     /translation="MADRPKRTYDITALQRGLRLLQLFSQSPRGLTAKQVATLSRLPV
                     STVHRFLANLVTSGFLSCDSDGNYHLGVACFAIGQAAVGQLDIRRLSLPFLRELNQQT
                     RETIHLTVRHGLSAVYVEKLDSPEPLRIHSRIGASVPLYCTAVGKVMLAYLPPEEQNR
                     LLQEIEIRRLTPNTVGSLQELKTELYRVRKNGYACDLEEHEIHIRCIAAPIWDHTGSV
                     QSSLSITAPVVRMPVTRLRQLAPMIQAAGLQISRELGYQPSPNPGSRPLADTKGPAEA
                     KLAKIAL"
     gene            2291..3907
                     /locus_tag="VEJ00_11595"
     CDS             2291..3907
                     /locus_tag="VEJ00_11595"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXX01853.1"
                     /translation="MSRRTVHIVVVFSCLSASGLDSSLSKIDVRAEDLLVQPPAANWI
                     SYHGDYSGRRYSSLSQVNLTTVSQLRAEWVFHVRNTNWLETTPIVVNGMMFVTAANDA
                     LALDAATGRVVWHHSRATSEGLIDDASRHLNRGVAVWRDRVYMETDNAHLLCLDARSG
                     NQIWDIPYADWNKNYGATSAPLVIKDKVLVGTSGGDDGVRGFVAAYDAMTGKLAWRFW
                     TIPGPGEFGSASWPGQAYLHGGGTTWMPGTYDPTLNLVFWGTSNPAPDFNDDVRPGDD
                     LYTDCVLALDADTGKLKWHFQFTPHDLFDYDAVETPILIDSVYQGEPRKLLVQANRNG
                     YLYVLDRASGKFLSAVPFVEKLNWAKGIDEQGRPIRSGIRPTPAGTRVCPGYTGATNW
                     YAPTYNESTHMVYLLALEECQTFFSQPTQAFHEGKEYYSTGVKRIPGETSQRILLAYN
                     LDTGRFAWKYPQSGNGHSSGGTMSTAGGVVFFGDDSGSFEAVDAQTGKPLWHFNTGQE
                     MSASPMTYAVGSKQYVALAAGSDVFSFALP"
     gene            3974..5227
                     /locus_tag="VEJ00_11600"
     CDS             3974..5227
                     /locus_tag="VEJ00_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXX01854.1"
                     /translation="MRLNSNRRGFLGTAAAVVASVFAPRKLFSSARGAAAAKTEVSGF
                     GQTGNPYEELGVTTVINCEGTMTMLGGSLLRPELEAVMAMAGQHFVNIPELEVAAGKR
                     IAEMLKLPEGYTALVTSGAAAAIQSGLAGILTGDNEAFIRQLPDLTGMKSEVIIQKSH
                     RNPFDHQLRSTGVKLIEIETRDQLKQAVNDRTAMMHFTNFANAAGQIKVDEWANLAKQ
                     YNIPCMNDAAADTPPVSHLWDYTNMGYDLVTFSGGKAIRGPQCAGLLIGRKELVANAL
                     LNNSPHEDTLGRSQKVGKEEIIGMVKALELYLKEDHDALAREWQSRLELVSREITKVP
                     GVATSFFTPDIANHVPHMHISWDSKISASPQEVSKLLRTAKPAIVMGGGEGRPGLSMN
                     SFMLKPGEDRIVAEHLARVLREHSA"
BASE COUNT         1111 a         1655 c         1552 g         1077 t
ORIGIN      
        1 atcgccgaaa tctcaactct ggcatcgtta accagagctg ccgcctggat tgtggcgcgc
       61 actggcttgg gatcagggaa cgcgctctta tacaccttgt tcatctctcc gaagtcatgt
      121 atgtcggcaa gatagaccgt aaccgaaaca acgtccttca gttcaaagcc tgccgctttc
      181 agtacccttt cgacgttgtc gagcgcggtc ttcgtctccg gcgcaatgcc gccctgagcc
      241 agtttccccg catcatcggt tccttcctgg cccgcaatat agagcgtgtt cccggcgaga
      301 accccatcgc tgaaaggcaa tccttttgcg ggtgcaaggt tgatggcttt gcggtcctgc
      361 gcataagtgg gatgccctaa cacaaccgtg gcaagaaaca aggccacaat caatccagac
      421 agccgcatgc tgtttcgtcg cattaatgtt ctccttcgcg aaccaattgc ttcagacttc
      481 gagcactctt ccaagcaaga gaaaacgatg gtacagtccc cgacatatat acaacgtgga
      541 ggacgcaatg aacaactctc atgtctgggc caggtgccgc ggaggcattg tgatgatcgc
      601 cgctttgggc gcggtctttg cagccgtgag cgaggtgcat ccgctccgcg cgcagtcgcc
      661 tgcgggagca gccacgccta cctccgacag tgacttgatt gcacaatttc gtcgcgtcga
      721 agtcgcttcg gtgtcggatg ctcttgagca gattaccggc aagcgcatgt acatgacgca
      781 ccgcatgcaa ccgatcttta caaccaagtt cgccggcttt gcgcggacgg tgcaactcaa
      841 aaaggacgaa ggcaacaagg acccggacgc gctgactgga atgctcgaag ctatcgacca
      901 gggatccacc gactcggtgt atgtgatggc ggtggaagac ggcgaggaca ttgccggcat
      961 gggtggcctc atgggcacgg caatggccgc gcggggctat gccggcgcgg tgatcgatgg
     1021 cggagtccgc gatgtcgcgt acttgcgtaa gattggcttc cccgtgttcg ccaccgggat
     1081 cgtaccctcc acgtccgtgc accactatcg cttcgccggt gcgcagattc cactggtctg
     1141 taacggcgtt cctgtaaacc cgggcgacat cgtggtcgcc gacagcgacg gcgtcgcggt
     1201 cgttcctcgg gctcacgcgc aagccgtgct caccttggcg cagcaaatgg acttcaaaga
     1261 gcactcgatg tatgcggtga tcgaacagct gaagtcaatt gtggctgcgg tgcagaaatt
     1321 cggacgcatc taggcccaag gccttgcgct gctcatactt tcatcagata attcagactt
     1381 tcatttcttt tcggtgcaga ctgcatccct tatggctgac cgccccaagc gcacgtacga
     1441 catcacagct ctgcaacgtg gactgaggct gctgcagctg ttcagccaat ctccgcgcgg
     1501 gctgactgcg aagcaggtcg ccacgctttc ccgtctgccc gtgagcacgg ttcatcgttt
     1561 tctggcgaac ctggtgacga gcgggttcct gagctgcgac tccgacggca actaccatct
     1621 gggagtcgct tgttttgcca tcggtcaggc ggcggtggga caactcgata tccgtcgtct
     1681 gagcctgcct ttcttgcggg aactgaacca gcagacacgg gagacgattc acctcaccgt
     1741 ccgccacggg ttgtccgcgg tgtacgtgga aaaactcgat tcgcccgagc ccttgcgcat
     1801 tcactcgcgc attggggcat ccgtgcctct ctactgcacc gccgtcggca aggtgatgct
     1861 cgcgtacctt cctccggagg aacagaatcg cctgttgcag gagatcgaaa tcaggcggct
     1921 gacgcccaat acagtaggca gcctgcagga gctgaagaca gagctctatc gcgtgcgtaa
     1981 gaacggttac gcctgcgatt tggaagagca cgagatccac attcgttgta tcgcggcacc
     2041 catctgggat cacaccggga gcgtgcagtc gagtctcagc atcacagctc cggtggtgcg
     2101 catgcctgtt acgcgactgc ggcaactggc acctatgatt caggccgccg gcctgcagat
     2161 ctcccgggaa cttgggtacc agcccagtcc gaatcccggc tcgcgcccgc ttgctgacac
     2221 caagggtccg gcggaggcaa agcttgccaa aatcgctctc tgagcgccgg acggctaccg
     2281 ggaagggctt gtgagcaggc ggacggtgca cattgtcgtg gtcttcagct gcctctccgc
     2341 ctctggcctc gattccagcc tctcaaagat tgacgtacgc gccgaagact tgctggtcca
     2401 gcctccagcc gccaactgga tctcctacca cggagactat tccggtcgaa ggtacagcag
     2461 cctttcgcag gtcaatctca ccacagtttc ccagcttcgt gccgaatggg tttttcacgt
     2521 ccgcaacacc aactggctcg agactacccc tattgtcgtg aacggaatga tgttcgtgac
     2581 cgcggccaac gatgccctcg ccctcgacgc cgcaactggc agggtcgtgt ggcaccactc
     2641 gcgggcaacc tccgaaggct tgatcgacga tgcctcccgc cacctgaacc gcggagtcgc
     2701 cgtgtggcgc gatcgcgttt acatggaaac cgacaatgcc caccttctgt gcctggacgc
     2761 gcgttccggc aatcagatct gggacatccc ctacgctgac tggaacaaga actacggcgc
     2821 caccagcgcg cccctggtca tcaaggacaa agtcctggtc gggacctccg gcggcgacga
     2881 cggtgtgcgc ggctttgtcg ccgcctacga tgccatgacc ggaaagttgg cctggcgatt
     2941 ctggaccatc ccgggtcccg gcgaattcgg ctcggccagt tggccgggcc aggcgtatct
     3001 gcacggtggc ggcacgacct ggatgccggg aacgtacgat ccgacactca atctggtttt
     3061 ctggggcacg agcaatcccg cccccgattt caacgacgat gtgcgccccg gcgacgatct
     3121 ttacaccgat tgcgtcctgg cattggatgc agataccgga aaactgaagt ggcatttcca
     3181 gttcaccccg cacgatctgt tcgactatga cgccgtcgaa acgccaatcc tgatcgattc
     3241 tgtctaccaa ggcgagccgc gcaagctcct cgtgcaggcg aaccggaatg gctatctcta
     3301 tgtgctcgat cgcgccagcg ggaagtttct ttcggccgtt ccgttcgtcg aaaaactgaa
     3361 ttgggcaaaa gggattgacg agcagggcag gccgattcgc agcggcatcc ggccgacgcc
     3421 cgccggaacg cgcgtctgtc cgggctacac tggcgccacc aactggtatg cgccgacata
     3481 taacgaatcc acccacatgg tgtatctcct ggcgctcgaa gagtgccaga ctttcttttc
     3541 ccagcccacg caggccttcc acgaaggcaa ggagtactac tccacgggcg tgaagcgcat
     3601 tcccggcgag acttcccaga ggattttgct tgcttacaac cttgacaccg gcagattcgc
     3661 ctggaagtat ccgcagtcgg gcaacggcca ctcctcaggc ggcaccatga gcacggccgg
     3721 cggcgtggtt ttctttggag acgattcagg atcgttcgag gcagtcgacg cgcaaaccgg
     3781 caagccgctg tggcacttca atacgggtca ggagatgagc gcctcgccca tgacctacgc
     3841 ggttgggagc aagcaatatg tggcgctggc ggcgggtagc gacgtgttca gcttcgcttt
     3901 gccgtgaatc agaatcgccc gtgaacggga tacggaaccg acggcctgta ttggtacgaa
     3961 aaggagaatc gtcatgcgac tgaattcgaa ccggcgcggc tttcttggca cggcggccgc
     4021 tgttgtggca tcagtctttg ctccccggaa gcttttctca tcggcccgtg gcgcagccgc
     4081 tgcaaagacc gaagtcagcg gctttggcca gaccggcaat ccctacgaag agcttggcgt
     4141 cacgaccgtg atcaactgcg aaggcaccat gacaatgctc ggcggttcgc tgctgcgccc
     4201 cgagctcgag gcggtcatgg cgatggcagg acagcacttc gtgaacatcc cggagctcga
     4261 agtcgccgcc ggcaaacgca tcgcggaaat gctcaagtta cccgagggct acacggcgct
     4321 ggtgacgtca ggtgcggccg cggcgattca atccggacta gcaggcattc tcaccggcga
     4381 caacgaggca ttcattcgcc agcttcctga tctgacgggc atgaaatcag aagtcatcat
     4441 ccagaagtcc caccgcaacc ctttcgatca tcagttgcgc tcgaccggcg tcaaactgat
     4501 cgagatcgag acccgcgacc agctgaagca ggcggtcaac gatcgtacgg ccatgatgca
     4561 cttcacgaac tttgccaacg ccgccggcca gatcaaagtg gacgaatggg caaatctggc
     4621 caagcaatac aacattccgt gcatgaacga tgccgcggca gacactcctc ccgtgtcgca
     4681 tctctgggac tacaccaaca tgggatacga tctggtgacc ttcagcggcg gcaaagcgat
     4741 ccgggggccg cagtgcgcgg ggttgctgat cggacgaaaa gaactggttg cgaatgcact
     4801 cttgaacaac agcccgcacg aagatacgct cgggcgcagc cagaaagtcg ggaaagaaga
     4861 aatcatcgga atggttaagg ctctcgagct ttacctgaag gaagaccacg atgcgctggc
     4921 cagagaatgg cagagccgcc tggagctggt ctcgcgcgaa atcacgaagg tcccgggcgt
     4981 cgccacgtcc ttctttaccc ccgacatcgc caaccacgta ccccacatgc acattagttg
     5041 ggattcaaaa atctcggcga gcccgcagga ggtttcgaaa ttactgcgaa ctgcaaaacc
     5101 cgcaatcgtt atgggtggcg gtgaggggcg ccccgggctg agcatgaact cgttcatgct
     5161 caagcccggc gaagacagaa tcgtcgccga gcaccttgcg cgcgtcttgc gcgaacactc
     5221 cgcctaactc tcgctgccag gagcctgctt gacggttggt catacaactc gcgtgcgatg
     5281 gctgctggtt ttctgccttt ttgtcctcag cgccgtgtct tacctcgatc gcgtaaacat
     5341 ttccattgcc ggcggatcga tcgccgacgc ttaccacctc aatgatgtgc agctg
//
LOCUS       DASPUL010000489         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2012649, whole genome shotgun sequence.
ACCESSION   DASPUL010000489 DASPUL010000000
VERSION     DASPUL010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2012649"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VEJ00_11605"
     CDS             <1..478
                     /locus_tag="VEJ00_11605"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HXX01855.1"
                     /translation="KELIQTLSVLPEYLRNQATESGAVIDYRDWHIQLGRRFRSLKLW
                     FVIRHYGVEGLQHHVRQHVKLAQQFAEWVRKDGRFELAAPVPLNLVCFRHKAGDAANQ
                     QIMDSLNRSGDLYLTHTKLNGKLTLRMSIGQTHTEERHVERAWKRIQEEADKQMCP"
     gene            complement(495..1418)
                     /locus_tag="VEJ00_11610"
     CDS             complement(495..1418)
                     /locus_tag="VEJ00_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXX01856.1"
                     /translation="MNSFGKIIRSVQAFGRELSRKASPIAPFRTTSIGVALGGGFARG
                     IAHIGVLKVLEEEGIPVRVVTGTSVGALIGACYCSGLSVAEMEDVARNTRFTTFARWT
                     LSRYGFASNDRMISFLTRTLKVQTFEELPILLGVTATDFNTGEAVVFHSGSIVDPVRA
                     SCAYPGMFLPVEIRGRHFVDGMLSHPVPTRPLRDMGADRVLGVHLKGTWAKGGAPRHL
                     FDVIGQSFAIAQDAMSSLWRNAADLVIEPDVGAFAYDDFKRSEDLIRVGEEAMRQALP
                     EVRKWLEHPAEAPAPAKDGRVVARPAPMPAD"
     gene            1635..2123
                     /locus_tag="VEJ00_11615"
     CDS             1635..2123
                     /locus_tag="VEJ00_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXX01857.1"
                     /translation="MVREISAGGVVVRHKDDEWWMAVIELPADSNAAVRQAKRSKGKP
                     ILCLPKGLVDPGEKALDAALREVREETGITAVAVTKLADIRYVYVRSWSDGERVFKIV
                     SFYLLRYESGRIDNISDEMRIEVARAQWVRLDEAPKLLAYRGEKQMARQALQYVEAHA
                     DV"
     gene            2270..>2757
                     /locus_tag="VEJ00_11620"
     CDS             2270..>2757
                     /locus_tag="VEJ00_11620"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXX01858.1"
                     /translation="MKRINKVAVLGSGTMGARIAAHFANASVPSYLFDIVPPDADAPA
                     RNKIAAAGLDAAKKSKPAAFMDPSLARFVTIGNFEDDLQKLADVDWIIEAVVENLDIK
                     RALLRKVEAIRKPGTIVTTNTSGLPVGKIAEGFSDEFRWAWFGTHFFNPPRYMRLLEI
                     IPT"
BASE COUNT          570 a          799 c          880 g          508 t
ORIGIN      
        1 caaagagctg atccagacgc tgagcgtgct gccggagtat ttgcgcaatc aggcgacgga
       61 atcgggggcg gtgatcgact accgcgactg gcacatccag ctgggacggc gctttcggtc
      121 gctgaagctg tggtttgtca tccggcacta cggcgtcgag ggcctgcagc atcatgtccg
      181 ccagcacgtg aagctggcgc agcagtttgc cgagtgggtg agaaaagacg gtcggttcga
      241 gctggcggcg ccggtgccgc tgaatctggt gtgcttccgc cacaaggcgg gagacgcggc
      301 gaaccagcag atcatggata gtctgaaccg cagcggcgat ctctacctca cgcacaccaa
      361 gctgaatggc aagctgacgc tgcgcatgag catcgggcag acgcacaccg aggagaggca
      421 cgtggaaagg gcgtggaaga ggattcagga agaagcagac aagcaaatgt gtccatgacg
      481 cggagccctg agtttcaatc tgccggcatg ggcgcggggc gcgccacgac ccggccatct
      541 tttgcgggag cgggcgcttc cgcgggatgt tccagccact tccgcacttc gggcaaagcc
      601 tggcgcatag cttcttcgcc gacgcggatg agatcttcgc tgcgcttgaa gtcgtcgtag
      661 gcgaatgcac cgacatccgg ttcgataacc aggtcggcgg cgttgcgcca cagtgacgac
      721 atggcatcct gcgcgatggc gaaggattgt ccgatcacgt cgaacaggtg gcgcggcgcc
      781 ccgcccttgg cccacgtgcc tttcagatgc acgcccagca cgcgatctgc gcccatgtcg
      841 cgcaacggtc gcgtgggtac gggatgggag agcatgccgt cgacaaagtg gcgtccgcga
      901 atttcgacgg gcaggaacat cccgggatag gcacagctgg cccgcaccgg atcgacgatc
      961 gaaccggagt gaaagaccac ggcttcccca gtgttgaagt cagtcgccgt cacgcccaga
     1021 agaatcggca gttcttcaaa cgtctggacc ttgagcgtgc gggtgaggaa cgagatcatg
     1081 cggtcgttcg aggcaaagcc gtagcgcgag agagtccagc gggcgaaagt tgtgaagcgg
     1141 gtgttgcggg cgacatcttc catctcggcg acggagaggc cgctgcagta gcaggcaccg
     1201 atcaaggcac cgacgctggt gccggtgacg acacgaaccg ggatgccttc ttcttcgagg
     1261 accttcagga ctccgatgtg ggcgattccg cgagcgaagc ctccgcccaa ggcgacgcca
     1321 atggacgtgg tgcggaacgg ggcaatcggg gaagccttgc gggagagttc ccttccgaag
     1381 gcctgcaccg aacgaatgat cttcccaaaa ctgttcacag cggaccccac atctggcctt
     1441 gcctttcgat ccggtcgggg ccgaaaggtc taaaacccct atctagcatg taagatgcca
     1501 caatcattag agaggctgta gggcacgtcc gtgccatttt gcggtgactt ttagggactt
     1561 tcctgcacgg taacaggtta ccggcgtgca ggctcgtgac gaaactcccg tattcctgct
     1621 aagttgcgga cagtatggtg cgcgagatct cggccggcgg cgtggtggtg cggcacaagg
     1681 atgacgaatg gtggatggcc gtcatcgaac tgcccgccga ttccaacgct gctgtccggc
     1741 aagcgaagcg atccaaggga aaaccgattc tgtgcctgcc caaaggcctg gtcgaccccg
     1801 gcgaaaaggc gctggatgcg gctctgcgcg aggtgcgcga agaaaccggg attaccgccg
     1861 ttgcggtcac caaactcgct gacatccgct acgtctacgt acgttcatgg agcgacggcg
     1921 agcgcgtgtt caagattgtc agcttttacc tgctgcgtta cgagtcgggg cgcatcgaca
     1981 acatctcgga cgagatgcgg attgaagtcg cgcgggctca gtgggtgcga ctggatgaag
     2041 cgccgaaatt gctggcctat cgcggcgaga agcagatggc gcgccaggcc ctgcaatacg
     2101 ttgaggccca cgcggacgtg tgatcccggc gctagataaa ttgggcgggg gcgattacca
     2161 ttgccagttc gctcaagtga gttctcgctg cgctcaggaa tgagggcgcg ctgtagcatc
     2221 gatcattttt catagccatt caccatggcc attcctatcg aggtccgtca tgaaacgcat
     2281 caataaagtc gctgttctgg gctccggaac gatgggcgcc cgcatagctg cgcattttgc
     2341 caatgccagc gtgccgagtt acctgttcga catcgtgcct cctgatgccg atgccccggc
     2401 acgcaacaag atcgccgctg ccggacttga cgcggccaag aagtccaagc cggccgcgtt
     2461 catggatcca tcactcgcgc gcttcgtcac cataggcaac tttgaggacg acttgcagaa
     2521 gctggccgat gtggactgga tcattgaagc cgtggtcgag aatctcgaca tcaagcgcgc
     2581 tctcctgcgg aaagtggaag ccattcgcaa gcccggaacc atcgtcacca ccaacaccag
     2641 cgggcttccc gtgggaaaga tcgccgaggg gttctcagac gagtttcgct gggcctggtt
     2701 cggcacgcac ttcttcaatc cgccacgcta catgcgcctg ctggaaatca ttcccac
//
LOCUS       DASPUL010000490         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_925952, whole genome shotgun sequence.
ACCESSION   DASPUL010000490 DASPUL010000000
VERSION     DASPUL010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925952"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VEJ00_11625"
     CDS             <1..627
                     /locus_tag="VEJ00_11625"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXX01859.1"
                     /translation="AVLYIVFMPDRSHAKVQRGKSKARILSSRTVYRGPVFSVTTDEV
                     AEPGGVRARRDVIRHSGSVVVLAVDDSGATPRILLERQYRHAANGYLWELPAGRIDPG
                     EPALAAARRELLEETGYSARHWRRILNFYATPGFVAETMSVYMATGLRKGEAQPEEDE
                     RIYIRMVPLPQAVRMVLRGTIRDAKTISSVLWLEHMTRTGTRRRKAKH"
     gene            886..1095
                     /locus_tag="VEJ00_11630"
     CDS             886..1095
                     /locus_tag="VEJ00_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HXX01860.1"
                     /translation="MERLKGTVKWFNNAKGYGFIGRADGPDVFVHYSAITSEGYKSLQ
                     EGDQVEFEITEGQKGPQAANVTKAS"
     gene            1302..>2251
                     /locus_tag="VEJ00_11635"
     CDS             1302..>2251
                     /locus_tag="VEJ00_11635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXX01861.1"
                     /translation="MDNKAIANILYETADLLEIDAQDSFRIRSYRNAAQAIENHSEQI
                     KDIIADPKKVLAIPGIGKGMLANLKELFDSGKLAVQAELLEKYHPSMLQLLKIQGLGP
                     KTIALIWSAYKVSDVDGVEKLAREGKIRELPRMGEKHEAKLLKAIEDYRRIGGRYLID
                     AAETEAEKLTEYLRKFSGIDKITPAGSLRRGRETVGDLDILVTGKACTSEEGRQKAIS
                     YVAKYPPLMDVIASGDNKISFRVRNGMQVDVRLLPPGSFGAAMQYFTGSKAHNVSLRQ
                     RALKKGYTLNEYSLATLEGEKPVAGKTEEEIYAKLDLDYIP"
BASE COUNT          492 a          721 c          639 g          399 t
ORIGIN      
        1 gccgtgcttt atattgtttt catgcccgat cgttcacacg ccaaagtgca gcgcggcaag
       61 tccaaagcac gcattctgtc ttcccgcacc gtctatcgcg ggccggtttt ctcggtgacc
      121 accgacgagg tcgccgaacc cggcggggtt cgcgcgcgcc gcgacgttat ccgccactcg
      181 ggttcggtcg tggtgttggc cgtcgacgat tccggggcca cgccccgcat tctgctggag
      241 cggcagtacc gtcacgctgc gaacggttat ctatgggaac tgccggccgg ccgcatcgat
      301 cccggcgaac cggcgctggc cgcagccagg cgcgaactgc tcgaggagac cggctactcg
      361 gcgagacact ggcgccgtat tctgaatttc tatgccaccc ccggattcgt agccgagacc
      421 atgtcggtct acatggccac gggcctgcgc aaaggcgagg cccagccgga agaagacgag
      481 cggatctaca tacggatggt tccccttcct caagccgtgc gcatggtgct tcgcggcaca
      541 atccgcgacg ccaagacgat ctcgtcggta ttgtggctgg agcacatgac gcgcacagga
      601 acacggcggc gcaaggccaa gcactgagag tcgtgccgtt gtcctctttg cgccctctgc
      661 ggcctttcct ttgcgaacct gcgatcaaag gctattaccg caaagtcgcg aaggaaaggc
      721 cgcagagaac gcaaagtaaa ccagaccccc gcatcccaac ccgaatcgcc gcctccccac
      781 acatttcctt gacagcccca ccctctcgct ccacaatgca tcctattggc tgtgggagca
      841 tgcccctggg cccccttcgg gggcgtgcag aaagcggggg atcacgtgga acgcctgaaa
      901 ggaaccgtca agtggttcaa taacgccaag ggttatggct tcatcggccg tgccgatggc
      961 ccggacgttt tcgttcacta cagcgccatc acttccgaag gctataagag cctgcaggaa
     1021 ggcgaccagg tcgagttcga aatcaccgaa ggccaaaaag gaccgcaagc cgccaacgtt
     1081 actaaggcgt cgtagctggg ccgtaagtgc gtaaggacag ccgccctcgg ctgtcgggtc
     1141 gagcgagctc gacagttcca agcagtctcc agtcctctgt catgctgagc ggagaaccat
     1201 cttcgccagg cgaagctcgt tcggagtcga agcatccctg cccattgcgg agtcgctgaa
     1261 tccctgttgg accattccgt ccatcttgtt atctttactc cgtggacaac aaggccatcg
     1321 ccaacattct gtacgagacc gccgatctgc tcgagatcga cgcgcaggat tcgtttcgca
     1381 tccgctccta ccgcaacgcc gcgcaggcca tcgagaatca ttccgagcag atcaaggaca
     1441 tcatcgccga tcccaagaaa gttctcgcca ttcccggcat cggcaagggc atgctcgcga
     1501 acctgaagga actcttcgat agcggcaagc tcgccgtgca ggctgagttg ctcgaaaaat
     1561 atcatccgtc gatgctgcag ctcttgaaga ttcagggact cggtccaaaa accatcgccc
     1621 tgatctggag cgcctacaag gtctcggacg tcgatggcgt cgagaagctg gcacgcgaag
     1681 gcaagattcg cgaactgccc cgcatgggcg aaaagcacga agccaagctg ctcaaggcga
     1741 ttgaagacta tcgccgcatc ggcggccgtt acctgatcga cgccgccgaa actgaagccg
     1801 aaaagctcac tgagtatctg cgcaagttct cagggatcga caaaatcaca cccgccggct
     1861 cgctgcgccg tggccgagaa accgtgggag acctcgacat cctcgtcacc ggcaaggcgt
     1921 gcaccagcga agaaggccgc cagaaagcca tttcgtacgt cgcgaaatat ccgccgctca
     1981 tggatgtgat cgcgagcggc gacaacaaga tcagcttccg cgtgcgcaac gggatgcagg
     2041 tcgacgtgcg cctgctgccg ccgggatcat tcggcgcagc catgcagtac ttcactggat
     2101 cgaaggcgca caacgtctcc ctgcgccagc gcgccctgaa gaaaggctac acgctcaacg
     2161 aatacagcct ggccacgctc gaaggcgaga agccggtcgc cggtaaaacc gaggaagaaa
     2221 tctacgccaa gctcgacctc gactacattc c
//
LOCUS       DASPUL010000491         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1318466, whole genome shotgun sequence.
ACCESSION   DASPUL010000491 DASPUL010000000
VERSION     DASPUL010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318466"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VEJ00_11640"
     CDS             complement(<1..907)
                     /locus_tag="VEJ00_11640"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HXX01862.1"
                     /translation="MYEKKPGVFMADWWDANGRRHRKQFANAASAREHEHRETALAHA
                     ERASREMKLASRSGGGKRSPARVVEGSMEPVKGKLGVMIPGMGAVATTFVAGVEAIRK
                     GLAKPIGSLTQMGTVRLGKRTDGRSPKIKEFVPLAGLDNLVFTGWDIFEDNMYEAASK
                     AGVLDQRLLDQLKPFLSSIAPQAAVFDHNYVKKIDGPNVKKGKNRMDLAEQLRDDIRS
                     FKKSSGASRLVTMWCGSTESFIEPTAAHQSVKAFEKALRENDEMIAPSMVYAYASLME
                     GVPFANGAPNLTVDLPVMHELSRKNE"
     gene            complement(1049..1183)
                     /locus_tag="VEJ00_11645"
     CDS             complement(1049..1183)
                     /locus_tag="VEJ00_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01863.1"
                     /translation="MLATRNRLNRNPYFHFHPLHALFSLVAALVLFAFLVWFLAIPAR
                     "
     gene            complement(1351..1797)
                     /locus_tag="VEJ00_11650"
     CDS             complement(1351..1797)
                     /locus_tag="VEJ00_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01864.1"
                     /translation="MLIPRRILATLLLAAVAAPVAARAQSSSDKPFVIEYYYKAKWGH
                     ADEFLKLFKKNHYPLLKKEVEMGRMLKVWMDQPRYHTTEDGRWDFRVTIVFKNAAIAN
                     ETIDEDPIKRQLWPDQETYQREEQRRFEILDAHWDLPVRTVDLDAK"
     gene            complement(2029..>2211)
                     /locus_tag="VEJ00_11655"
     CDS             complement(2029..>2211)
                     /locus_tag="VEJ00_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01865.1"
                     /translation="RHVHCSKAIIHCPVILSEVAVREANGNAVEESLPRALAEGRFTV
                     LRYCNRLLGLRGRAAL"
BASE COUNT          412 a          676 c          665 g          458 t
ORIGIN      
        1 cttcgttctt tcgtgaaagc tcgtgcatga ccggcaggtc cacggtcagg ttcggcgcgc
       61 cattggcaaa cggaacgcct tccatcagcg aagcataggc gtagaccatg gagggagcaa
      121 tcatctcgtc gttctcgcgc agcgccttct cgaacgcctt caccgactgg tgcgcggcgg
      181 ttggctcgat gaagctctcg gtcgagccgc accacatggt gaccagacgg ctggccccgc
      241 tcgacttctt gaagctgcgg atgtcgtcgc gcagttgctc ggccagatcc atcctgttct
      301 tacccttctt cacgttgggc ccgtcgatct tcttgacgta gttgtggtca aacaccgccg
      361 cctgcggcgc aatcgacgac agaaagggct tcaactggtc gagcaggcgc tgatcgagca
      421 cgcccgcctt cgatgccgct tcgtacatgt tgtcttcgaa gatatcccag ccggtgaaga
      481 ccaggttgtc gagcccggcg agcggcacga attccttgat cttcggcgaa cgcccgtcgg
      541 tgcgcttccc cagccgcacg gtgcccatct gggtgagcga accgatgggc ttggccaggc
      601 ctttgcggat ggcctcgacg ccggcaacaa acgtggtcgc caccgcgccc atgccgggga
      661 tcatcacccc cagcttgccc ttgaccggtt ccatggaacc ctccacgaca cgggctggcg
      721 accgttttcc gccaccgctg cggctggcca gcttcatttc acggctggcc cgctcggcat
      781 gcgccagggc ggtctcgcga tgctcgtgct cgcgagctga cgctgcattg gcaaactgct
      841 tgcgatgacg tcggccgttg gcatcccacc agtcggccat aaacactccc ggtttctttt
      901 cgtacatcgg cgtctccatt ggtgacggtg gctggtcacc gccacccatc gtgggatagt
      961 ctcacgtggc cgggaaatct ggcaatggca ggcttgcgga atgcgggaaa cggcacttca
     1021 accgcgcagc ggcgcagtga gcgggcggct agcgcgccgg gatcgccagg aaccacacca
     1081 gaaaggcaaa caacaccagc gcggcaacca gcgaaaacag cgcatgcagc ggatggaaat
     1141 gaaaataggg attgcgattc agacgattcc gggtcgccag catacgacct cctctgcacg
     1201 tcttgcacgt gccaatcagg cgactttgac tagcctattg tcgcactatt tgcacgttct
     1261 ggcccgtctt ttttaccgcc gagcacgccg agagcgctga gatctcgcac ttctcttcct
     1321 cggcgatctc tgcgtcctcg gcggtagatg ctatttggca tccagatcga cagtccttac
     1381 cggcaggtcc cagtgggcgt cgagaatctc gaagcggcgc tgctcctcac gctggtaggt
     1441 ttcctgatcg ggccagagct gccttttgat cgggtcttcg tcgatcgtct cattggcgat
     1501 ggcggcattc ttgaaaacaa tggtcacgcg gaagtcccac cggccgtctt cagtggtgtg
     1561 ataacgcggc tggtccatcc agaccttcag catgcgcccc atctcgactt ctttcttgag
     1621 cagcggatag tggttcttct tgaacagctt aaggaactcg tcggcgtgtc cccatttggc
     1681 cttgtagtag tactcgatga cgaaaggctt gtcggatgac gactgcgcgc gggcggcgac
     1741 tggcgcggcc acggcggcca acagtagtgt ggcgagaatg cggcgcggga tgagcatggg
     1801 tcctcctcgg gatccagttg cttgtacact aagcgcattg ccgggtcaaa gttgggcaga
     1861 gtcatcgcgg ggggacttcc aggggggagg cggaaagtga catggaagag cggcccttca
     1921 agggccgcga caaggttgaa tccgagatgc cctttagggc tggtagtcgt tttgagcatg
     1981 tccgccacgg aggctaaagc cccacaactt cacgcgcctg acgcggcgct aaagcgccgc
     2041 tcttccacgt agtcccagca gccggttgca ataccgcaga accgtaaagc gcccttcggc
     2101 caaggcgcgg ggtagggatt cttcgactgc gttgccgttc gcttcgcgaa cagcaacttc
     2161 gctcagaatg acaggacaat ggattatcgc ctttgagcaa tgaacatgcc g
//
LOCUS       DASPUL010000492        11434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_927349, whole genome shotgun sequence.
ACCESSION   DASPUL010000492 DASPUL010000000
VERSION     DASPUL010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11434)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927349"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VEJ00_11660"
     CDS             <1..545
                     /locus_tag="VEJ00_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01866.1"
                     /translation="PSTGPVVKTYTFDDVINGLNQVAPYDWRGFWSERLTNHGPGAPL
                     GGIEGSGWKVVYDEYQSEMDANRMGITRSIDATYSLGLSLRDDGGIRDTIEGMPAAKA
                     GIGPGMQLIAVNGRQFSPDVLRDALRAAKIGSTPIELLIENTDYYKTYKIDYHGGEKY
                     PHLVRDESKPDLLSDIMRAK"
     gene            654..2891
                     /locus_tag="VEJ00_11665"
     CDS             654..2891
                     /locus_tag="VEJ00_11665"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HXX01867.1"
                     /translation="MSVIRFTPIALLLVAFAVPTCCQTFILGFTPASSARESEIEKKF
                     KSIPNAHEERRQHRIFTAEPHVAGSKRNNELADYIAAQWKKQGLEDVIIRRYDVYGTN
                     PKSASLEMISPVHYTATLREAPIPGDDDSKNPAISGAWLGMSISGEVAAPVIYAHSGN
                     PEDYDLLRQNGISVKGKIVLVRYSNPYSYRGFKALTAQREGAAAMLVYSDPQEDGYNK
                     GKVDPDGPWGPEYHIQRGAITYDFMVPGDPQTPGWASVPGAKRIPIEDAVSAPKIMAL
                     PLSWHDAKPLLENLDGPVAPDSWQGGLGFPYHFGGERVRGHLKIEMENGLQPYYVVEG
                     RIRGSDLPDEWVVLGNHRDAWVFGGVDPSSGTASMMEMTRALGELKQQGLRPRRTIVV
                     CSWDGEEVGLTGSTEWGEQFADDLRQKAVAYINVDEATSGPDFYGQAVASLAPMLEET
                     TRALDDPGGKTLYESWKARVERDRKTGQQSGQFNDSGAANQSLADTRIGSGSDHTVFL
                     NFIGMPVLGLGFVGDYGVYHSAYDDFFWMNHFGDPGYKYHTLMSQLWGVTALRLANAD
                     LLPFDFVTYAQNIRQFVTDLADKNKLLNMGGARAESPSAISMWGQPPSAVQPGEARQP
                     LDLIPILTAVDNFESAGKKLNESIAHKLASGPIDPNLAATLNHGMMQLERNWLNPDGI
                     PGRPWFKHILYGARYTYAHLELPGLTEAVEKQDWPTAKQQREILERALEKNTKLLEEL
                     NGAVK"
     gene            complement(3054..3365)
                     /locus_tag="VEJ00_11670"
     CDS             complement(3054..3365)
                     /locus_tag="VEJ00_11670"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HXX01868.1"
                     /translation="MKKRRSREATGVRSATKPKTRRAKGRRRRRTVKPWDLNLAAPVR
                     DSRLQGAKIGDFYRPLKKPITVRLDADVLAWFKKDGRRYQTRINAALRKVMEREMKQS
                     G"
     gene            complement(3475..4254)
                     /locus_tag="VEJ00_11675"
     CDS             complement(3475..4254)
                     /locus_tag="VEJ00_11675"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXX01869.1"
                     /translation="MTSEIKTSGSNTFGLKSPLRTLSASAAKMRLLKRLRCTLRYEKK
                     AWAAGAAMVAGVDEVGRGSLFGPVVAAAVILDPAHRIRGLRDSKLLLPERREVLAERI
                     RAHCIAWTIASVDAARIDAINIYQASRVAMREAVLQLAPAADYLLVDALRLDCELPQD
                     AIIHGDALSASIAAASIVAKVERDRMMREWDAVYPLYGLASHKGYSTPQHLAALREHG
                     PCPLHRQSFAPVWQNPVPQDAFGFMAEDEGTEEVTAEDLAG"
     gene            4547..5320
                     /locus_tag="VEJ00_11680"
     CDS             4547..5320
                     /locus_tag="VEJ00_11680"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXX01870.1"
                     /translation="MDRTTNTSPESGLLTGKLVHLALPVRWGFVGQEGRVALETACTY
                     DIHPRGARLVTARELSVGDLVMVERGRNKAMCQVVWAADPNSPLRGQFSVQCVEEGRT
                     PWDDELRQMEERYQPIVLDGPQQRSLASGFGRSDHNRRRRPRFYVEGQAEVIDGVQRV
                     AGEVQQLSEFGARIAAKEPLRPGTDFRLMLNVFDISLALKAQVKYLVDNLGMGVEFQE
                     IRLGDRPLLHHVLSKLRTKRVEEFAEVEVVTEPLMAAAG"
     gene            complement(5584..5934)
                     /gene="rplS"
                     /locus_tag="VEJ00_11685"
     CDS             complement(5584..5934)
                     /gene="rplS"
                     /locus_tag="VEJ00_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXX01871.1"
                     /translation="MSNLIIEKLLAKTQRTDIPAFRPGDTVRVHVKIKEGDKERLQAF
                     EGVVIKRDNGPQASFTVRKISFGQGVERIFPIHSKVIDKVELVRSSKVRRARLFYLRG
                     LKGKAARLRDVEKA"
     gene            complement(6040..6855)
                     /gene="trmD"
                     /locus_tag="VEJ00_11690"
     CDS             complement(6040..6855)
                     /gene="trmD"
                     /locus_tag="VEJ00_11690"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXX01872.1"
                     /translation="MKADIVTIFPDFFRGPLDHGITRRASEMGLVNIEVHDLRQFTHD
                     RHRTVDDRPFGGGEGMVLKPEPIFECLESLGLARREQRLSGAAKESVILLSAQGERFT
                     QKIAAELTALDRIVLICGRYEGVDERVADFLADRELSIGDYVISGGELAAAVVIEAVT
                     RLLPGAVGNEASTRQESFTAKAGEVTESAGPDSTCSSNGLLDYPHYTRPAEFREMAVP
                     EVLINGNHQQIRRWRREQALRKTLRKRPDLIESVQLSDEDRKILERVKTGLSS"
     gene            complement(6902..7534)
                     /gene="rimM"
                     /locus_tag="VEJ00_11695"
     CDS             complement(6902..7534)
                     /gene="rimM"
                     /locus_tag="VEJ00_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HXX01873.1"
                     /translation="MVAVHTDVDPETSSGFITLARIVKTQGRHGEVAAEVHSDVPDRF
                     AGGMKLFAVDKAEARRELEIEELWPHKGLLVLKFAGVDSISDAEALIGSELQVPRAER
                     AKLEEGWTYVSDLIGCMVFDQGREIGRIEDVQFGAGEAPLLIVIDSAGKRFDVPFAEA
                     YLERVDVARHEVRMKLPEGMLDVNAPVTDDEKCRFERARLKPRRRGSKQE"
     gene            complement(7731..7973)
                     /locus_tag="VEJ00_11700"
     CDS             complement(7731..7973)
                     /locus_tag="VEJ00_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HXX01874.1"
                     /translation="MTEPSGDICALIELIAKSLVDAPDEVFVEKFDDGVIELEVAEAD
                     VGKVIGRQGRTARALRALLAAAGHRAHKRYQLEIIE"
     gene            complement(8099..8350)
                     /gene="rpsP"
                     /locus_tag="VEJ00_11705"
     CDS             complement(8099..8350)
                     /gene="rpsP"
                     /locus_tag="VEJ00_11705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HXX01875.1"
                     /translation="MIRLARIGARKQPYYRIVVIEKARARNGRPVEVVGTYNPRTSPA
                     SVELKRDRVEYWVSKGAQMSERVNKLFSKTPAAAPAPAA"
     gene            complement(8889..9767)
                     /locus_tag="VEJ00_11710"
     CDS             complement(8889..9767)
                     /locus_tag="VEJ00_11710"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXX01876.1"
                     /translation="MSYVDVGKENSTSTHLYYEDHGAGQPVVLIHGYPLSSASWEKQV
                     PVLLKAGYRVIAYDRRGFGKSSQPTTGYNYDTFAQDLHKLITHLKLKNFVLVGFSMGG
                     GEVARYIGKHGSKDVAKAVIMGGVPPYLLKTADNPEGVDAAIFAGIEKAVATDRYAFF
                     TEFFKNFYNTDVHLGKRVSEQAIQASWNTAAIASATASLACVPTWGEDFRKDVAKIDV
                     PTLVVHGDSDRIVPFSAAGQRTAKLVKGAELVVIKDGPHNVAWTHADEVNSALLNFLA
                     KPAAKGQAAPKKKAAA"
     gene            10018..11199
                     /locus_tag="VEJ00_11715"
     CDS             10018..11199
                     /locus_tag="VEJ00_11715"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXX01877.1"
                     /translation="MQAVYILSAVRTPIGKFGGTLASLTASDLGVVAAKAAMERAGVR
                     PEQVEETIFGNARQAGGGPNPARQVSVRSGVPQEVPAFTVNKACASGMKAIALAYQAI
                     LLGEANCILAGGTESMSRVPYLLDQARWGYRMGTQELVDGMYRDGFFCPLAKMVMGET
                     AEALAEQYKISREEQDELALASQTRAARAIAAGRFEAEIVPATIEGKKGATVFSRDEH
                     PFPDVSPEKLAKLPPVFSKAGTISAGNSSGITDGAAAVVVASEYFVKQNNLKPLARVQ
                     AVASAGVDPKTMGIGPVPALKKLEDKHGLRVHDFGLIELNEAFAAQVLACDRVLNMNR
                     ERLNVNGGAIALGHPIGCTGTRIVVTLLHEMMKRNTKRGVATLCVSGGMGMALALENV
                     V"
BASE COUNT         2204 a         3579 c         3449 g         2202 t
ORIGIN      
        1 ctccctcgac cggccccgtg gtcaagacct acaccttcga cgatgtgatc aacggcctca
       61 accaggtcgc tccctacgac tggcgcggct tctggagcga gcgcctcacc aaccacggcc
      121 ccggcgctcc gctggggggc atcgaaggca gcggctggaa agtcgtctac gacgagtatc
      181 aatccgaaat ggacgcgaac cggatgggca tcacccgctc catcgacgcc acctactctc
      241 tcggcctcag cctgcgcgac gacggcggaa tccgcgacac catcgagggc atgcccgcag
      301 ccaaggccgg aatcggcccc ggaatgcagc tgatcgcggt caacggccgc cagttctccc
      361 ctgacgtgtt gcgcgacgct ctgcgcgccg ccaagatcgg cagcaccccg atcgagctgc
      421 tgatcgaaaa caccgactac tacaagacct acaagatcga ctaccacggc ggcgaaaagt
      481 atccgcacct ggtgcgcgac gaatccaaac ccgacctgct gagcgacatc atgagagcga
      541 aatgaaaccg ctttgaaaag gcgcagtatg gcgggtgccc catttctcgc gcgttttgtg
      601 cgcgagaagt ggggatctta acttccgcca ctgcgataca atctcatccg cccatgagcg
      661 tcatccgctt cactccgatc gcactattgc tggtcgcatt cgcggtcccg acctgctgcc
      721 agacttttat cctcggtttc actccggcat cgtccgcccg cgaatccgaa atcgagaaaa
      781 aattcaaatc catcccgaac gcccacgaag aacgccgcca gcatcgcatc ttcaccgcag
      841 agccccacgt cgccggctcc aagcgcaaca acgagctggc cgactacatc gccgcccaat
      901 ggaagaagca aggtctcgaa gacgtcatca tccgccgcta cgacgtctac ggcaccaatc
      961 ccaaaagcgc gtcgctcgaa atgatctcgc ccgtacatta cacagccacg ctgcgcgaag
     1021 cccccattcc cggcgacgac gactccaaga accccgccat cagcggcgca tggctcggca
     1081 tgtccatctc cggagaagtc gccgcgcccg tcatctacgc ccacagcggc aatcccgaag
     1141 actacgatct cctccgccag aacggcatca gcgtgaaagg caagatcgtg ctcgtgcgct
     1201 actccaatcc ctacagctat cgcggattca aagcgctcac cgcccagcgc gaaggcgccg
     1261 ccgccatgct cgtctacagc gacccgcagg aagacggcta caacaaaggc aaagtcgatc
     1321 ccgacggacc ctggggcccc gaataccaca tccagcgcgg cgccatcact tacgacttca
     1381 tggtccccgg cgatccgcaa acccccggct gggcctcagt tcccggcgcc aagcgcatcc
     1441 cgattgaaga tgccgtctcc gcaccaaaga tcatggcact gccgctctcc tggcacgacg
     1501 ccaagcccct gctcgaaaac ctggacggcc ccgtcgctcc cgattcctgg caaggcgggc
     1561 tcggctttcc ctatcacttc ggtggcgagc gcgtaagagg ccatctcaaa atcgaaatgg
     1621 agaatggcct gcaaccgtat tacgtcgtcg aaggtcgcat ccgcggatcc gaccttcccg
     1681 acgaatgggt cgtcctcggc aatcaccgcg acgcctgggt tttcgggggc gttgatccct
     1741 ccagcggcac cgccagcatg atggaaatga cgcgtgcctt aggagaactc aagcagcaag
     1801 gcctgcgccc gcgccgcacc atcgtcgtct gcagttggga cggcgaagaa gtcggcctca
     1861 ccgggtcgac cgaatgggga gagcaattcg ccgacgacct tcgccagaag gccgtcgcct
     1921 acatcaacgt cgacgaagcc accagcggcc ccgacttcta cggccaggcc gtcgcctcgc
     1981 ttgcgcccat gctggaagaa accacgcgcg cgctcgatga cccgggcggc aagactctct
     2041 atgaatcctg gaaagcccgc gtcgagcgcg atcgaaaaac gggccagcaa tccgggcaat
     2101 tcaacgactc cggcgccgcc aaccaatcgc tcgccgacac ccgcatcggc agcggcagcg
     2161 accacacggt cttcctcaac ttcatcggca tgcccgtcct cggcctcgga ttcgtcggtg
     2221 actacggcgt ttatcactcc gcctacgatg actttttctg gatgaaccac ttcggcgacc
     2281 ccggctacaa gtaccacacg ctcatgagcc agctctgggg cgtcaccgcc ctgcgcctgg
     2341 ccaacgccga cctgctcccc ttcgacttcg tgacgtatgc gcaaaacatc cgccaattcg
     2401 tcaccgacct cgccgacaaa aataagcttc tcaacatggg cggagcgcgc gcagagtcac
     2461 cttctgcaat atccatgtgg ggacagccgc cctcggctgt ccagccgggc gaagcccgac
     2521 agccgctcga cctcatacca attcttaccg ccgtcgacaa tttcgaatcc gccggaaaaa
     2581 agctgaacga atccatcgcg cacaaactcg cctccggccc aatcgacccc aacctcgccg
     2641 caacgctgaa ccatggcatg atgcagctcg agcgcaactg gctcaacccc gacggcatcc
     2701 ccggacgccc ctggttcaag cacatcctct acggcgcccg ctacacctac gcccacctcg
     2761 aactccccgg cttaaccgaa gctgtcgaaa agcaagactg gcccactgca aagcagcagc
     2821 gcgaaatcct cgagcgtgcg ttggagaaaa acacaaagct gttggaagaa ttgaatggcg
     2881 cggtcaaata aacagcgggt gccccatcct agcgcgttct ttgcgctagg gtggggattt
     2941 tgactgttag ggacagagcc ctcggacgta tgcaccctgg aagcgcggcc cttcaggggc
     3001 gcgtcggaca cgttaaggaa agacccgggg cttcagcccc ccgaacgtag gaactacccg
     3061 gactgtttca tctccctctc catcaccttc cgcagcgccg cgttgatccg cgtctgatag
     3121 cgccgcccgt ccttcttgaa ccacgccagc acatccgcat ccagccgcac cgtgatcggc
     3181 ttcttcagcg gacggtaaaa atccccgatc ttcgcgccct gcaacctcga gtcgcgcacc
     3241 ggcgccgcca gattcaaatc ccacggcttg accgtccgcc ttcttcgccg gcctttcgct
     3301 cgccgcgttt tgggcttcgt tgcggagcgc acgccggttg cttccctgct cctgcgcttc
     3361 ttcataacct gctcatccca ttgcacttgc tgaaatatcc gaccaaagta tgtacaggat
     3421 tgtacatgaa agtgttcctg caggaacact gtggaaaact tccctggccc gcccctaacc
     3481 tgctaaatct tcggcagtta cctcttcggt gccttcgtcc tcggccatga agccgaacgc
     3541 atcctgcggc acagggttct gccacaccgg cgcgaacgac tgccgatgca gcgggcaggg
     3601 accatgctcg cgcagcgccg ccaggtgctg cggcgtgctg tatcccttgt gcgaggccag
     3661 cccatacaac ggatacaccg cgtcccactc gcgcatcatg cggtcgcgtt ctaccttggc
     3721 cacgatcgac gccgccgcaa tcgacgccga cagcgcgtcg ccatgaatga tggcatcctg
     3781 aggcagctcg caatccagcc gcagcgcatc gaccaacagg tagtctgccg cgggcgcgag
     3841 ctgcagcacc gcttcccgca tcgccacgcg cgacgcctga tagatgttga tcgcgtcgat
     3901 gcgcgccgca tctaccgaag caatcgtcca cgcaatgcag tgcgctcgaa tgcgctcggc
     3961 cagcacctcg cgccgctccg gcaagaggag cttcgagtcg cgcagacccc gaatgcggtg
     4021 cgccggatcg agaatcacgg ccgctgctac caccggccca aacagcgacc cgcggcccac
     4081 ctcatcgacg ccggccacca tggcagcgcc cgctgcccac gccttcttct catagcgcaa
     4141 ggtgcagcgc agccgtttca gcaggcgcat cttcgcggcg gacgccgaca gcgtccgcaa
     4201 cggcgacttg agtccaaacg tgttgcttcc agaagtcttg atttcagaag tcaagggagt
     4261 tgcgaacatc ttcgtcgacc gagcgcaatc acgcaagcac agcttttcca ccggcgctca
     4321 acaactctgt tgtcattccg actaactttt attgtcattc cgaactgctt cagcggtgag
     4381 gaacctgctt tttgaagcct cccagcgcgc cgcgagcggc gatcgggcac gcgccccacc
     4441 acgtccgtaa tgccgcaaag taaccacctc ggcgattacg cacgtacatt gtccatcggg
     4501 acatgtactg gtaacgtggc agcagttcca ttgtgggcca acagacttgg atcgcacaac
     4561 caacacgtct ccggaaagcg gactgctgac gggcaaactc gtgcacctgg cgttgccggt
     4621 gcgctggggc ttcgtgggtc aggaagggcg cgtcgcgctc gagacggcat gcacctacga
     4681 cattcatccg cgcggcgcgc gcctggtgac ggcgcgcgaa ctgagcgtag gcgacctggt
     4741 gatggtggaa cggggacgga acaaggccat gtgccaggtg gtctgggcgg cggatcccaa
     4801 ctcgcccttg cgtgggcagt tcagcgtgca gtgcgtcgag gaaggccgta cgccctggga
     4861 cgacgagttg cggcagatgg aggagcgcta ccagcccata gttttggacg ggccgcagca
     4921 acggtccctg gcgagcggat ttggccgcag tgatcacaac cgccgtcggc gtccgcgatt
     4981 ttatgtggaa ggccaggctg aggtcattga tggcgtgcag cgtgtagctg gcgaggtgca
     5041 acaactctcc gagttcggag cgcgtatcgc ggccaaggag ccgctgcgtc cgggcaccga
     5101 cttccggctt atgctcaacg tcttcgacat cagcctggcc ctgaaagcac aggtgaagta
     5161 tctcgtcgac aatctgggga tgggagtcga gtttcaggag atccggctgg gcgaccggcc
     5221 gctgctccac catgtgttga gcaagctgcg gaccaaacgc gtcgaggagt tcgccgaggt
     5281 ggaagtggtc accgagccgc tgatggcagc ggcaggatag ccctcaactc cagcgctatg
     5341 ggaagggtcc accggggctg aagccccggt atcttcttgc cccttgacgc ggcgctgaag
     5401 cgccgatctt ccacgtcacc gcaagcgcca ttgcgtaagc agatgacgcc ctgcctgtac
     5461 ctgagcgagg cgtcgtttcg acggacgttc ctatcgaatc tctccgggca gatctgaacg
     5521 ccgtgtacaa gcatgcgaag ctttgctccg cggacagccg aggcggctgt ccctacagcg
     5581 tcgctaggct ttttccacgt cgcgcaggcg ggcagctttg cctttcaggc cgcgcagata
     5641 gaacaggcgc gcgcggcgca ctttcgacga gcgcaccagt tccaccttgt cgatcacctt
     5701 ggagtgaatg gggaagatgc gttcgacgcc ctggccgaag ctgatcttgc ggacggtgaa
     5761 gctggcctgc gggccgttgt cgcgcttgat gaccacgcct tcgaaggcct gcagacgctc
     5821 cttgtcgcct tccttgatct ttacgtgaac ccgcacagtg tcgccggggc ggaatgcggg
     5881 aatgtcggtg cgctgcgtct tggccagcag tttttcgatg atgaggtttg acatggtcgt
     5941 tgtccttcag tgcaatagat tccatttttc gtttgtcatt ctgagcgcag cgaagaacct
     6001 ctgccggcag cggcaggttg cataggtcct tcgcttcgct caggatgaca agccggtttt
     6061 gactcgttcc aaaatttttc tgtcttcgtc gctgagctgc acgctctcga tcaaatcggg
     6121 gcgcttgcgc agcgtctttc tcaacgcctg ctcgcggcgc cagcggcgaa tctgctggtg
     6181 gttgccgttg atcagcacct ccggcactgc catctcgcga aactcggccg ggcgcgtgta
     6241 gtgcggatag tcgagcagcc cgttcgagct gcaagtcgag tccggacctg cgctttccgt
     6301 aacctcgcca gccttcgccg taaagctctc ctggcgcgtc gaagcctcgt tgccaaccgc
     6361 tcccggcagc aggcgcgtta ccgcttcgat gaccacggcc gcggccagtt ctccgccgct
     6421 gatcacgtag tcgccaatcg aaagttcgcg gtcggcgaga aagtcggcga cgcgctcgtc
     6481 gacgccctcg taacgtccgc agatcagcac gatgcggtcg agcgcagtca gctccgccgc
     6541 gatcttctgc gtgaaccgtt cgccctgcgc cgagagcagg atcacgcttt ctttcgcggc
     6601 gcctgatagg cgttgctcac gccgcgccag cccgagcgat tccaaacatt caaagatcgg
     6661 ttccggcttc aggaccatgc cttcgccgcc gccgaagggg cggtcatcca cggtgcggtg
     6721 ccggtcgtgc gtgaactggc gcagatcgtg gacttcgatg ttcaccaggc ccatttccga
     6781 agcccggcgg gtgatgccgt gatcgagcgg tccgcgaaag aagtccggga agatcgtgac
     6841 gatgtcggcc ttcatttttc gcaaacccaa aacgctttga ggggcgcggc ttaagccgcg
     6901 gctattcttg tttggaacct ctacggcgcg gcttaagccg cgccctttca aacctacatt
     6961 tctcgtcgtc agtgaccggc gcgttgacgt ccaacatgcc ctcgggaagt ttcatgcgca
     7021 cctcgtggcg cgctacgtcg accctttcca aatacgcttc ggcgaaagga acgtcgaacc
     7081 tcttgcccgc gctatcgatc acaatcagca gcggcgcttc gccggctccg aactgcacgt
     7141 cttcgatgcg gccgatttca cggccctgat cgaagaccat acatccgatc agatcgctaa
     7201 cgtaggtcca accttcttcc agcttggctc gttcggcgcg cggaacctga agctcagatc
     7261 cgatcagcgc ttcggcgtcg ctgatcgaat cgacgcccgc gaacttcaga accagcaggc
     7321 ccttgtgcgg ccacagttct tcgatctcca gctcgcgcct agcttccgct ttgtccacgg
     7381 cgaacagctt catgcccccg gcaaatcgat ccgggacgtc gctgtgaacc tccgccgcga
     7441 cttcgccgtg gcggccctgc gttttcacga tgcgggccag ggtaatgaag ccgctgcttg
     7501 tttccggatc cacgtctgtg tgaacagcga ccacaggggc taaagcccct ctcttctccg
     7561 agctttacgc ggcgcggaag cgccgctctt ccacatcggt tccgtcactc acgtgtccgc
     7621 gctttacccc agggctaaag ccccaatatt cgttcagcgc tttgcggcac gactaaagtc
     7681 gtgcccttcc cgtccgtgct tcgtgaagct ccctcgaaaa gtcggcgtat ctactcgatg
     7741 atctcgagtt ggtagcgctt gtgggcgcgg tggccggcgg cggcgagcag ggcgcgcagc
     7801 gcgcgggcag tgcggccctg gcgtccgatg accttgccta catcggcttc ggcgacttcg
     7861 agttcgatca caccatcgtc gaatttttcg acgaagactt catcgggcgc gtcgaccagc
     7921 gacttcgcaa tcagctcaat cagcgcgcag atgtctccgg agggctcggt cataatcatt
     7981 ctcgatgtgg tgaggttccc tcagcggcga aagccgtaaa ttcttgcgcc tctacggcac
     8041 gactaaagtc gtgcccttcc cggccgtccc caattcaaca gttcgtgaag gcggcgactc
     8101 aggcggccgg agcaggcgct gcggccggag tcttggagaa cagcttgttc acacgctccg
     8161 acatttgtgc gcccttcgac acccagtact cgacgcggtc gcgcttcagc tcgaccgaag
     8221 cggggctggt acggggattg taagtcccga ccacctcgac cgggcggccg ttgcgggcgc
     8281 gcgccttttc gatcaccaca atgcgatagt acggttgttt gcgcgcaccg atgcgcgcca
     8341 gacgaatcat taacacgaga cggttatcct ttatctttag gttccggaag agtccggcgg
     8401 ggctaagaca aactcttatt atgccggagg ttaggggaga atgcaacgag gaccgttggc
     8461 ggctgtgtat cggtttgaat tcaggccgga ctcgtggagc ggtccgcgcg tcctttgatg
     8521 aggagtgcaa agaacacgat gctggcgcag aatatgctcg ctataagcgg aaggaaaggc
     8581 gatctccctt gggattttgc aagcctccaa accccgtaca tgaaggccgc tttaccgctt
     8641 accaaaatca cgtagaaaag cacataagcc atgtttagcg attctacgcc gctgattcca
     8701 gcaggatcaa aattcaaaca gacacgttaa tccttggtca ttcagcctct cagtatttgt
     8761 catcctgagc ggcgctcttt gccgcgaagg atccgggcag tttgctgctg ccggagtgtt
     8821 gcatgggtcc ttcggcccgc ggagagcgcg ggcctcagga tgacatgggt ttgagtgtgt
     8881 tgctggtact atgcggcggc tttcttcttc ggcgccgctt gtcctttcgc ggccggctta
     8941 gccaggaagt tcagcagcgc gctgttgact tcgtcggcgt gggtccaggc aacgttgtgc
     9001 gggccgtctt tgatgacgac cagttctgca cccttgacca gcttcgcagt gcgctggcct
     9061 gcggctgaga acgggacgat gcgatccgag tcgccgtgaa cgacgagcgt gggcacgtcg
     9121 atcttcgcga catctttgcg gaaatcttcg ccccaggtcg ggacgcacgc gaggctcgcg
     9181 gtggccgagg cgatggcggc tgtgttccag ctggcctgaa tagcctgctc gctcacgcgt
     9241 tttccgagat ggacgtcggt gttgtagaaa tttttgaaga actcggtgaa gaacgcatag
     9301 cgatcggtgg cgacggcctt ctcgatgcct gcgaagatgg cggcgtcaac gccttcgggg
     9361 ttgtcggcag tcttcagcag gtagggcgga acgcctccca tgatcaccgc cttggcgacg
     9421 tctttcgaac cgtgcttgcc gatgtagcgg gcgacctcgc ctccgcccat ggagaatccg
     9481 acgagcacaa agtttttgag cttgaggtgc gtgatgagtt tatgcagatc ttgggcgaag
     9541 gtgtcgtagt tgtagccggt ggtgggctgg ctggacttgc cgaatccgcg gcggtcgtag
     9601 gcgatgacgc ggtagccggc cttgaggaga acggggacct gcttctccca ggatgcgctg
     9661 ctgagaggat atccgtggat caggacgacc ggctgaccgg cgccgtgatc ttcgtagtag
     9721 agatgggtgg acgtggagtt ttcttttccg acatcgacgt aggacatgga ggtcctcctt
     9781 aagtgagttt ttgacgagga cgaaaactgg ttccactgga gacgcagtgg cagttattga
     9841 ttgtagaaga aagggtgagg cttggggtat ccggggtctt tcccacccgt tcggagcgcc
     9901 gcgcttgctc agggtaggct ctagccgcca agaacgccaa gggtggggca agcggacgga
     9961 cgagagcgtc cgcccctaca cagatcctgc tagactcgag tgttttggag gtgcaccttg
    10021 caggcggtct atattctctc ggcggtgcgg acgccgatcg ggaagtttgg cgggacgctg
    10081 gcttcgctga cggcgtcaga cttgggcgtg gttgcggcga aggcggcgat ggagcgggcg
    10141 ggggtgcgtc cggagcaggt cgaggagacg atctttggca acgcgcggca ggcggggggc
    10201 gggccgaatc cggcgcggca ggtgtcggtg cggtcgggcg ttccgcagga ggttccggcg
    10261 ttcacggtga acaaggcgtg cgcgtcgggg atgaaggcga ttgcgctggc ctatcaggcc
    10321 atcttgctcg gcgaggcgaa ctgcattctg gcgggcggga cggagtcgat gtcgcgcgtt
    10381 ccttatttat tggatcaggc gcgctgggga tatcggatgg gcacgcagga actggtggac
    10441 gggatgtacc gggatggatt tttttgtccg ctggcgaaaa tggtgatggg tgagacggcc
    10501 gaggcgttgg ccgagcagta caagatttcg cgcgaggagc aggatgagct tgcgctggcg
    10561 tcgcagacgc gggcggcgcg ggccattgcg gcggggagat tcgaagcaga gattgtgcca
    10621 gcgacgattg aaggaaaaaa aggcgcaact gttttcagcc gcgatgagca tccgtttccg
    10681 gatgtgagcc cggagaagct cgcaaaactg ccgccggtct tctcgaaagc gggaacgatc
    10741 tcggcaggaa attcttcggg gattaccgac ggggcggcgg cggtggtggt cgcgagcgaa
    10801 tattttgtga agcagaacaa tctgaagccg ctggcgcggg ttcaggcggt ggcctcggcg
    10861 ggcgtggatc cgaagacgat gggcattgga ccagtgccgg cgcttaagaa acttgaggac
    10921 aagcatgggc tgcgggtgca tgacttcgga ttgatcgaac tgaacgaggc gttcgcggcg
    10981 caggtgctcg cgtgcgatcg cgtgctgaat atgaatcgcg agcggctgaa cgtgaatggc
    11041 ggcgcgattg cgctggggca tcccatcggg tgcacgggga cgcggattgt ggtgacgctg
    11101 ctgcatgaga tgatgaagcg gaatacgaag cgcggcgtgg cgacgctgtg cgtgagcggg
    11161 gggatgggga tggcgctggc gctggagaat gtggtgtagc tttcagacgc tttctgtcct
    11221 gcggaaaagc aggttcctcg cgcaaagagc gcgctcggaa tgacaaaaag tctagccttc
    11281 gcgtcccgga tgcagtgatg atccgcgcca gatccctcgc gtcgctggta agaacgcgac
    11341 gcttcgggat gacgccggca gttgcagaaa tcacccatat ggaaggcaat ccgcaatttc
    11401 gtgcggccgc agaaaccgac gctgaggcgt tgct
//
LOCUS       DASPUL010000493         2986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2014643, whole genome shotgun sequence.
ACCESSION   DASPUL010000493 DASPUL010000000
VERSION     DASPUL010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014643"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1805
                     /locus_tag="VEJ00_11720"
     CDS             195..1805
                     /locus_tag="VEJ00_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01878.1"
                     /translation="MQIEVDFSKVEGPVSPVWNYFGYDEPNYAYAANGKKLLAELAAL
                     DAAPVYVRVHNLFTSGDGSASLKWGSTNVYSEDASGKPIYSWTILDRIFDTFHAAGIR
                     PLVEIGFMPEALSTHPEPYRHDFPRTPVGEIYTGWASPPRDYQKWSELVFQFADHLRK
                     RYGDAEVKSWLWEVWNEPDIGYWKGTREEYFKLYDFSVEAVLRALPEGTIGGPDTTGP
                     GSANAVDFLTAFLEHCAHGKNYATGKTGSHLDFISFHPKGSPTWQGDHVQMGITRQLA
                     AIQQGFKIVASFPEWRKTPIILGESDPEGCAACSAKENPRNLYRNGPLYGAYTVEVLN
                     NALALSNREHINLRGAVTWAFEFEDQPYFAGFRELATNGVDKPVLNAFRMLGLLGPER
                     LTATSSAGLTTEEVVRDGVRGKPDISVIATRKEREIEILVWNYHDDDLPVPPALIDLA
                     ITGLPTEAKRGLLEHFRVDSSHSNAFAAWKEMGSPQSPSDSEYKQLESAGQLQLLTSP
                     VWIEIEHGSAHLKFDLPRQGISLLRLAW"
     gene            complement(1819..>2986)
                     /locus_tag="VEJ00_11725"
     CDS             complement(1819..>2986)
                     /locus_tag="VEJ00_11725"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HXX01879.1"
                     /translation="EGHAVRAMYACCGATDYYMETGDDTYLKTLNTLWNDMTTTKMYV
                     TGGVGSRSDGEAFGDAYELPNFRAYGESCAAIGNMMWNWRMLAVTGDAKFTDVLERAL
                     YNGINSGMSLDGTLYCYRNPLAFDPSTGDKIRNPWYDVTCCPPNLERTFGSLPGYFYS
                     TSSDGIYVHLYDNSELNWHLENGTGLKVVQKANYPWEGGVEITVTPAEASDFTLYLRI
                     PGWTDHAQVSVNGKPVAGAMPGQYLPIQRRWSPGDVIHMTLEVVPQVIEANPRIAEDS
                     GRVAIQRGPLIYCLEELDQPSGVSLSDVAVDPGRRPAEQFQTEFKSDLLGGMVVLHHT
                     GAVYERGALEKALYTRYVSGPVKTRRVPLTFIPYYAWANRQATSMQVWNLLLRA"
BASE COUNT          627 a          923 c          846 g          590 t
ORIGIN      
        1 cgaaccatcc ggatttcccc agcacggaat tgaggccggg cgagcgctac cacaccgtca
       61 ccgtcttcag cttctcgacg cgttgagccg gaatgtttcg ccgccgcgca gacaatcgag
      121 ccagccgctg tgcgcgggtt cctctccttt tgctgatcag ccaactttgc tggcctcagc
      181 aggcatccga gccagtgcaa attgaggtgg acttcagcaa ggttgagggt ccggtctcac
      241 cagtgtggaa ttacttcggt tacgacgagc ccaactacgc gtacgccgca aacggaaaga
      301 agctgctcgc agaattagcc gcgttggatg ctgccccggt ttacgtgcgc gttcacaacc
      361 tcttcaccag cggcgacgga tcggcatcgc tgaagtgggg ttcgaccaac gtgtattccg
      421 aagacgcctc cggaaaaccg atctatagct ggaccattct cgatcgcatc ttcgatacgt
      481 tccacgcagc gggaatcagg cccctggtcg agatcggctt catgccggaa gcgctctcca
      541 cgcaccccga accgtatcgt cacgactttc cccggactcc cgtcggcgaa atctataccg
      601 gatgggcctc tccgccaagg gactaccaga agtggtccga gctggtcttt cagttcgccg
      661 atcacctgcg caaacgctac ggcgatgccg aagtaaagag ctggctgtgg gaagtgtgga
      721 acgaacccga tatcggctac tggaaaggga cgcgcgaaga gtatttcaag ctctacgatt
      781 tcagcgtcga agccgtgctg cgcgcgctgc cggaaggaac gatcggagga ccagacacca
      841 ccgggcccgg cagcgcaaac gccgtcgact ttctcacagc gtttctggaa cattgcgcgc
      901 acggcaagaa ttacgcgacc ggcaagacgg gatcacatct cgacttcatc agctttcatc
      961 ccaaaggatc gccaacctgg cagggcgatc acgtgcagat gggcatcacg cgccagctcg
     1021 ccgcgatcca gcagggattc aagatcgtcg cttcctttcc cgagtggcgg aagactccca
     1081 tcattcttgg ggaatccgac ccggaaggct gtgcggcctg ctcggcaaaa gagaatccga
     1141 ggaatctcta tcggaacgga cctttgtatg gagcgtacac cgtcgaagtc ctgaacaacg
     1201 ccctcgcgct gtcgaatcgg gagcacatca accttcgcgg agcggtgacc tgggccttcg
     1261 aatttgaaga tcaaccttat ttcgcgggct tccgcgaatt ggcgacgaac ggcgtcgaca
     1321 agcccgtgct gaacgcattt cgcatgctgg ggctgctcgg cccagagcgc ctaaccgcaa
     1381 caagttcggc agggttaacc acagaagaag ttgtgcgcga cggggtgcgc gggaagccgg
     1441 atatcagcgt catcgcgacg cgaaaagagc gagaaatcga gattctcgtc tggaactacc
     1501 acgatgatga tctgccagtt ccgcctgcgc tcatcgacct ggccatcacc ggactcccga
     1561 cagaggccaa acgcggcctg cttgaacact tccgcgtcga ttcgagccac agcaacgcgt
     1621 tcgccgcgtg gaaagaaatg ggatcacccc agtcaccttc cgatagcgag tacaaacaac
     1681 ttgaaagtgc aggacagttg caactgctca cctcaccagt gtggattgaa atcgaacacg
     1741 gcagcgcgca cctgaagttc gatctgccgc ggcaaggcat ctcgctgttg cggcttgcgt
     1801 ggtagtgttc gagcacggct acgctctcag cagcaggttc cacacctgca tggaagtcgc
     1861 ctgccggttc gcccatgcgt agtatgggat gaaagtcaat ggaaccctgc gcgtctttac
     1921 cgggccgctc acgtagcgcg tgtagagagc tttctccaac gccccacgct catagactgc
     1981 gcctgtgtga tggaggacca ccatcccgcc gagcaggtcg ctcttgaact cggtctgaaa
     2041 ttgttctgcc ggccgccgcc cgggatcgac cgcaacgtcg ctcagcgata caccgctcgg
     2101 ctgatcgagt tcctccaggc agtaaatcag cgggcctctt tgaatcgcca cgcgcccgct
     2161 gtcttctgcg atgcggggat tcgcttcgat gacctggggc acaacctcca atgtcatgtg
     2221 aattacgtct cccggcgacc agcggcgctg gatgggcagg tactgccccg gcatcgcccc
     2281 ggccacgggt tttccgttca ccgaaacttg cgcatgatcg gtccagccgg gaatgcgcag
     2341 gtacaacgtg aagtctgacg cctccgccgg cgtgaccgtg atttccacgc caccttccca
     2401 cggataattt gccttctgca ccaccttgag ccctgttcca ttctccaggt gccaattcag
     2461 ctccgaattg tcgtaaagat ggacatagat tccatcggaa cttgtgctgt agaaatatcc
     2521 cggcagggaa ccgaacgtgc gctccaggtt cggcggacaa caggtcacgt cgtaccaggg
     2581 attgcggatc ttgtcgccgg tcgacggatc aaatgccagc ggattgcgat agcagtagag
     2641 tgtgccgtcg agcgacatgc cggaattgat cccgttgtac agagcgcgct ccagcacatc
     2701 ggtgaacttt gcatctcccg tgacggccag catgcgccag ttccacatca tgttgccgat
     2761 ggcggcgcag ctctcaccat aggcccggaa attgggtagc tcgtaggcgt cgccgaacgc
     2821 ttcgccgtcg gacctcgacc ccacaccgcc ggtcacatac atcttggtcg tggtcatgtc
     2881 gttccacagc gtgttcagcg tcttcaaata ggtatcgtcg cccgtctcca tgtagtagtc
     2941 ggtggcgccg cagcacgcat acatagcgcg aacagcgtgg ccttcg
//
LOCUS       DASPUL010000494         5209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_364533, whole genome shotgun sequence.
ACCESSION   DASPUL010000494 DASPUL010000000
VERSION     DASPUL010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364533"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VEJ00_11730"
     CDS             complement(<1..767)
                     /locus_tag="VEJ00_11730"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXX01880.1"
                     /translation="MKLAALKPLLPILTLALASVCSSAAFASTPPHKRPKSDKDINAI
                     GRRNIALAQNWYSLEKEKELGAQLSAAFERSVAILQDPAANAYVEALTQRIAQNSDAH
                     MPVTVRIVNRDDVSATTVAGGHIYVTRGLLLQMKSEGELASILAREVAHIALRSATWE
                     LTQRNLVQLLEVPLVWPTGDGRTQPANAGTQGLGSSLTLLHFRRIDEADADYFGVQYL
                     YKSGYDADCFLSAIQKVPPQDSSPPIAEAFSPFPLLP"
     gene            complement(879..1310)
                     /locus_tag="VEJ00_11735"
     CDS             complement(879..1310)
                     /locus_tag="VEJ00_11735"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HXX01881.1"
                     /translation="MIDINKNIIRRVLEEGGNRHDIAVIRELYPNVVYHAPLVGELRG
                     EAYRRFVASVLDAFPDAHWTIEDQIAEDDRVMTRWSFVGTHKERFMDIAPTGKKVRIS
                     GMCIDRIVEGKIVEEWEEFDGLGMMRQLGVVPAHIEEPVAA"
     gene            complement(1371..2666)
                     /locus_tag="VEJ00_11740"
     CDS             complement(1371..2666)
                     /locus_tag="VEJ00_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01882.1"
                     /translation="MTTRALASMAVIGLLAATALPTRCLAQQHNNQLPRYSVIDLGTL
                     PGGTYSLAGGLSNAGWVEGFSTLDNDTALHAVLWRHDGPIDLGTMGGTSSDSGWRPNN
                     WGDAGGGSDTATSDPMGEDFCGHGTNLICLPFLWRHNTGKMTPLPTLGGNNAWAAGVN
                     DQDEVAGVSENTIAEPTCPPAQPGQTPQIFQWKPVHWSNGRIHALPTFRKDPVGEALA
                     INASGDIVGWSGDCVNVRHALLWRNGKAIDLGNLGGKFNNEALDINSWGQVVGFSDVP
                     QDATGHAFLWQWGFMIDLGTLPGDVGSSGDGINNWGQVVGGSYDADENSRAFLWQDGV
                     MTDLNTLIPADSPLYLLEATGTINDAGEIAGQAVQLSTGEIHAFLLIPRYDKAIGDAT
                     NATLAVGAPRPHVTIPENVRQSLRRARIARFKGLQMGKP"
     gene            complement(2940..4628)
                     /locus_tag="VEJ00_11745"
     CDS             complement(2940..4628)
                     /locus_tag="VEJ00_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01883.1"
                     /translation="MPQDAPQSAAPRFNPARLAVLAAAVVVYLVILHLLRASHAIAIA
                     GLGFQYAVLLPCALWTLNGLRNSGEREITLPPVALALACAYVLLVIPLAKHESSWPAN
                     AADESCYAFQARILGTGRFIADPLPGTSTTVRSTPDELNYQNHILIPRGWFTHFPPGW
                     PALLLVGQRLGAPWIVNPLLGLILLWLMWTIGRETFSLGTARLAILMAVLSPFFLVNA
                     VRQLSHMACAVLVAGACLLVFRGLARLQILPVACALALLVLACPIRPYTAFCEALVLG
                     GAALLCSRHDRKFAVQIVLAGAVAALAALLIMMAYNHATTGHATVSPYAARVGSDVPS
                     ELTFQPHFIFYFLRRWGWSTFTETILSTFPFLFLLAAYALWRETDKPREARILACVFA
                     ILPLAHLLHTENSYSFFGSRFHFEGLFAAVILGARGAELLARRWALPRSAVLAVVALF
                     AAMQIADIRGMVRSLWNLNPQYHAIKAAVADLDGKVPLVFLQSGGGYNARFINFNQAD
                     WKLAPTVYLIDADPSNRDAWACRFDRADWVVLHFDAKGDAEQTLGHTRCPEQRP"
     gene            complement(4726..>5209)
                     /locus_tag="VEJ00_11750"
     CDS             complement(4726..>5209)
                     /locus_tag="VEJ00_11750"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXX01884.1"
                     /translation="RNFASRLAAVLSAAGITGAALAPRSEGQTKSDDSVRKLGDDGKP
                     ADPHDFITPIVLHNGLIYVAGQGAHDPGPAEQFEIGHHTTIVMDKIKKLVETGGGTVD
                     TILQLGVFLADIKYYDGMNKVFKTYFPNGGPARTTVAVAALPGNSLVEINCIAAVVKK
                     "
BASE COUNT         1020 a         1422 c         1782 g          985 t
ORIGIN      
        1 tcgggcaaca gtggaaatgg gctgaatgcc tcggcgatcg gaggcgacga gtcctgtgga
       61 gggacttttt gaattgcgct gagaaagcag tcagcgtcgt aaccagactt gtaaagatac
      121 tggacaccga agtagtcggc atcggcctcg tcaatacgcc ggaagtgaag cagtgtaaga
      181 cttgaaccca gcccctgcgt gccagcattc gctggttggg tacgaccgtc cccggtcggc
      241 cataccaagg ggacctccaa caattgcact aaattgcgct gggtaagctc ccaggtggct
      301 gagcgtagcg cgatgtgcgc gacctcgcga gcgagtatgc tggcgagttc gccttcgctc
      361 ttcatctgca gaaggagccc gcgcgtgaca tagatatggc cgccggcaac ggtcgttgcc
      421 gagacatcgt cgcggtttac gatgcgaact gtgaccggca tgtgcgcatc tgagttctgc
      481 gcgatgcgtt gcgtcagagc ttcgacatat gcgttcgccg caggatcctg caagattgcg
      541 acggatcgtt caaaagcggc cgagagctgc gctcccagct ctttttcctt ttccagggaa
      601 taccagttct gagcgagcgc aatattgcgg cgtccaatgg cgttgatgtc tttatcggac
      661 ttgggtcgtt tgtgaggcgg ggttgaggcg aaggcagcgc tggagcacac gcttgccagc
      721 gcgagagtca agatcggcag caacggctta agggcggcga gcttcaaaaa cttactccac
      781 agccggcagg tcctgtaact ctcaatgata actccaccgg agatgcaacc accccgcgag
      841 caaggtgcag gtggcctaat tctggccacc tgcaagcctt atgcggcgac cggctcctcg
      901 atgtgggcag gcacgacgcc gagctggcgc atcatgccca ggccgtcaaa ctcctcccac
      961 tcctcgacga tctttccctc gacgatgcgg tcaatacaca tgccgctgat gcggaccttc
     1021 ttgccggtgg gtgcgatgtc catgaacctt tccttgtggg tgccgacaaa gctccaacgg
     1081 gtcatgactc tgtcgtcctc ggcgatctgg tcttcgattg tccagtgggc atcggggaat
     1141 gcatccagga ctgaagcgac gaacctccgg taggcttcgc cccgaagttc gcccaccagc
     1201 ggcgcgtgat agacgacgtt gggatacagc tcacggatga ccgctatgtc gtgccggttg
     1261 ccgccttctt cgagcacgcg gcgaataatg ttcttgttga tgtcaatcat gatgatctcc
     1321 tttgtcttgc tcgtggttca tcggtgtttc aacgtcgatc agcttgctta tcagggtttt
     1381 cccatctgca gccctttgaa tcgggcaata cgtgcccgcc gcagggactg acgaacattt
     1441 tccgggatag taacgtgcgg tcttggcgcg cccactgcga gtgttgcatt ggtcgcgtcc
     1501 ccgatggctt tgtcatatct cgggatcagc aaaaaggcat gaatctcgcc ggtactgagt
     1561 tgcacggcct ggccggcgat ttcgcccgca tcgttgatgg tgccggttgc ttccagcaag
     1621 tacagcgggg agtcggcggg gatgagtgtg ttgagatcgg tcatcacgcc gtcttgccag
     1681 aggaaagcgc gggagttctc atcggcgtca tatgatccgc cgaccacctg gccccagttg
     1741 ttgataccgt cccccgagct cccaacgtcg ccgggcagag tgcccaggtc gatcatgaag
     1801 ccccactgcc agaggaaggc atgcccggtt gcatcctggg gcacgtcgga aaatcccacc
     1861 acctggcccc agctgttaat gtcgagggcc tcgttattga acttgccgcc caggttccca
     1921 aggtcgattg ccttgccgtt tcgccagagg agagcatgcc gaacgttcac gcagtcaccg
     1981 gaccagccga cgatgtcgcc cgaggcattg atcgccaatg cctctccgac cgggtctttt
     2041 cggaacgtag gcagggcgtg gatccgtccg ttgctccagt ggacaggttt ccactggaag
     2101 atttgaggag tctgacctgg ctgagcaggc gggcaggttg gctcggcgat ggtgttctct
     2161 gagacgccgg caacttcatc ttgatcgttg actccggccg cccaggcgtt gttgccgccc
     2221 agagtgggca gaggagtcat ctttccggtg ttgtgccgcc acaggaaggg cagacaaatg
     2281 agattggtgc catggccgca gaaatcttcg cccatagggt cggaagtagc ggtgtcggag
     2341 ccgccgccgg catctcccca gttgttgggg cgccacccgg aatcgctgct ggtgcctccc
     2401 atggtgccca ggtcgatcgg tccatcatgg cgccaaagga cggcgtggag ggccgtgtcg
     2461 ttgtccaacg tggagaagcc ctcgacccag cccgcgttgc tgagtccgcc agcaaggctg
     2521 taggtgccgc cggggagagt acccaggtcg atcacgctat agcgcggaag ttggttattg
     2581 tgctgctgcg cgaggcaacg cgtgggaagg gccgttgccg cgagcaggcc tatcacagcc
     2641 atgctcgcca atgctctggt agtcatgatg cctccatgag gagcgctcga gttgaactgc
     2701 ggggttgaag gtgaagatca gctcgcctca gggggtgatg cgtccgtggc gggggctctt
     2761 cggggaggcg agagaattta ccgcgccatt gtcagtgcga gggagtgcgt ttgtcagtga
     2821 cgggggtcac cgactgaacg ctggacggag ctatgcgaca ttgtcaggct tgacgggcga
     2881 aagcgtttga ccgcaaagct cgctaaggaa ggccgcaaag gacgctaaga actacaagat
     2941 cacggacgct gttccgggca gcgggtgtgg ccgagggttt gttccgcatc tccttttgca
     3001 tcgaaatgca gcacgaccca atcggcgcgg tcaaaacggc aggcccaggc atcacggttg
     3061 gacgggtcgg cgtcgatcag gtagacggtc ggggcgagct tccagtcggc ctgattgaag
     3121 ttgatgaagc gggcgttgta tccgccgccg ctttggagga agaccagcgg gaccttgcca
     3181 tcgaggtcgg ccaccgcggc tttgatggcg tgatactggg gattcaaatt ccacagcgag
     3241 cgaaccatgc cgcggatgtc ggcgatctgc atggcggcga acaacgcgac caccgcgagc
     3301 accgccgaac gtggcagggc ccagcggcgg gccagcaact ccgcgccgcg cgcgccgaga
     3361 atgacggcgg cgaacagtcc ttcgaagtga aagcgcgatc cgaagaagga atatgagttc
     3421 tccgtgtgga gcagatgcgc gaggggcaag atggcgaaga cgcaagcgag aatgcgcgcc
     3481 tcgcgtggtt tgtccgtctc ccgccagagc gcgtaggcag ccagaagaaa caggaagggg
     3541 aaggtggaga ggatagtttc ggtgaaggtg ctccatcccc agcggcggag aaaatagaaa
     3601 atgaagtgag gctggaaggt gagttcggag gggacgtcgg atcctacgcg cgcggcgtag
     3661 ggagatacgg tggcgtggcc ggtggtggcg tgattgtagg ccatcatgat gagaagcgcg
     3721 gcgagggcgg cgacggctcc ggcgagaacg atctgcacgg cgaatttccg gtcgtggcgt
     3781 gagcacaaga gcgcggcgcc gcctaacacg agggcttcgc agaaagcggt gtaggggcgg
     3841 atcgggcagg ccagtacgag caaggcgagg gcacaggcaa ccggaaggat ttgcaggcgc
     3901 gcgaggccac ggaacacgag caggcaggcg ccggccacca gcaccgcgca cgccatatgc
     3961 gagagctggc gcacggcgtt gacgaggaag aacggagaca ggaccgccat cagaatcgcc
     4021 aggcgggctg tgccgagcga gaaggtctcg cgcccgatgg tccacatcag ccacaacaga
     4081 atcagcccga gaaggggatt gacgatccag ggagcgccga gccgctggcc gacaagcaga
     4141 agagcgggcc atcccggtgg gaaatgtgtg aaccatccgc gcgggatgag gatgtggttc
     4201 tgatagttga gctcatcggg agtgctgcgc acggttgtgc tggtgccggg caggggatcg
     4261 gcgatgaagc gtccggtgcc gaggatgcga gcctgaaagg cgtagcagga ttcgtcagcg
     4321 gcgttggcgg gccagctgga ttcgtgtttc gccagcggga tgacgagcag aacataggcg
     4381 caggcgagcg ccaaggccac ggggggcagg gtgatttcgc gttcaccgga attgcgcaag
     4441 ccattgagcg tccagagtgc gcagggcaag aggacggcgt attgaaaccc aagaccagca
     4501 attgcgatag cgtggctggc gcgcagcaga tgcaggatga cgaggtaaac cacgacggcc
     4561 gcggccagca cggcgagccg agcagggttg aagcgggggg ctgcggattg tggggcgtcc
     4621 tggggcactg gcggctggct ttctcgcaga gtaccacagc aacgcggcgc tgaagcgccg
     4681 ctcttccacg taatcacgca tctatgtcac gtgcaacgca tgcgtttatt tctttaccac
     4741 ggcggcgata cagttgattt ccaccagcga gtttcccggc agggcggcga cggcgactgt
     4801 ggtgcgggcg gggcctccgt tggggaaata ggttttgaag accttgttca tgccgtcgta
     4861 gtacttgatg tcggcgagga agacgccgag ctggaggatg gtgtcgacgg tgccgccgcc
     4921 ggtttcgacc agcttcttga tcttgtccat gacaatggtg gtgtggtggc cgatttcaaa
     4981 ttgttcggcc gggccgggat cgtgcgcgcc ctggccggcg acatagatca ggccattgtg
     5041 caggacgatg ggcgtgatga agtcgtgcgg gtcggcgggc ttgccgtcgt cgccgagttt
     5101 gcggacgctg tcgtcggatt tggtttggcc ttcggagcgg ggcgccagcg cggcgccggt
     5161 gatgcccgcg gcagagagga cagcggcgag acggctggcg aaatttcgg
//
LOCUS       DASPUL010000495         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1868257, whole genome shotgun sequence.
ACCESSION   DASPUL010000495 DASPUL010000000
VERSION     DASPUL010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868257"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1081)
                     /locus_tag="VEJ00_11755"
     CDS             complement(218..1081)
                     /locus_tag="VEJ00_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXX01885.1"
                     /translation="MNNRLLIALPLAAMLSFPAIAQTTSSTQTQPAATTTTSTDNATG
                     KAPLAPPSREGFWGRVNPFARKKYVQRQTEPIRDRVNELDELTSANSKAIKDTDSRAQ
                     AGIKLASDKADTADQHAIDAGNKATMAQQTAQQVTTRVQTVENVVGNIDQYKASNQTE
                     IRFRPGQTVLSKNAKDALDQMATGVKGQHGYIIEVQGFSSGKGQAAIQTSQKMAESVV
                     RYLVLNHEIPVYRIYLVGMGNAPTPDETAKTKHVSGGRVEVSLLKNDLEQLSANTGAP
                     ATTADQQQQPK"
     gene            1498..>2633
                     /locus_tag="VEJ00_11760"
     CDS             1498..>2633
                     /locus_tag="VEJ00_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXX01886.1"
                     /translation="MGTGHLAAASGWGKTWASISVLLGVALLPGNGWAGGATTPHTQM
                     SPPGVASARVPLERLELYSDLAVTWMQDYLRIDTTNPPGNEMRAVAFYKKILDQEGIE
                     NRTFEYAPGRGDLWARLSRNAPSTGAEAKRPIILLNHMDVVTSDASHWKVPPFSGEMK
                     DGYLWGRGAQDMKDEGLAQLATMVMLKREKIALDRDVIFLAVADEEVDGTGTDWFINH
                     QRELLENAEFLINEGGENLLENGRVAYVGVDVGEKTTYWLHVVAHGTPGHGSRPNPDS
                     APNRLVRALNKIIAYRTPIRVLPVVQEFLRDMAPYQTPERAAQYRNIRKAVEDKRFEE
                     EIERDESLNFLLRDTISLTMMGGSEQTNVIPPEAWANLDVRILPG"
BASE COUNT          514 a          677 c          900 g          542 t
ORIGIN      
        1 aaacgataga ggtccacttg cacgagcggt cagggctcct tttgatggga gcagtataga
       61 cccgaaggga aatctgctca gggcgatttt ccggacagga ggaaaagcag gtttctcctc
      121 gcttcgctcg tcgaatgaca agactatcgt agcgccgcaa atgagaaagc ctctcgcatt
      181 cgcgagaggc ttttttcagg gggagtgagc ttggaactta cttcggctgc tgctgctgat
      241 cggctgtggt agcgggcgcc ccggtattgg cggagagctg ctccaggtcg tttttcagca
      301 ggctgacttc cactcggccg ccgctgacgt gcttggtctt ggcggtctca tcaggagtgg
      361 gggcgttgcc catgccgacg agataaatgc gatagaccgg aatctcgtgg ttgagcacga
      421 ggtagcgcac gaccgactcg gccatcttct gcgaggtctg gatcgcggcc tggcccttgc
      481 ctgacgagaa gccctgcact tcgatgatgt agccgtgctg gcccttcacg ccggtcgcca
      541 tctggtcaag cgcgtccttg gcattcttgc tgaggacggt ctggccgggg cggaagcgga
      601 tttcggtctg gttcgaggcc ttgtactggt cgatgttgcc gaccacgttc tcgaccgtct
      661 gcacacgcgt cgtgacctgc tgcgcggtct gctgcgccat ggtcgccttg ttgccggcgt
      721 cgatggcgtg ctgatcggcg gtatcagcct tgtcggaggc gagcttgatg ccggcctggg
      781 cgcgcgaatc ggtgtccttg atcgccttgc tgttggccga ggtcagctca tcgagttcgt
      841 tgacgcggtc ccggatgggc tcggtctggc gctggacata tttcttgcgc gcgaagggat
      901 tcacgcgtcc ccagaagccc tcgcgggatg gcggagcgag aggagccttg ccggtcgcgt
      961 tatcggtcga tgttgtcgta gtcgcggcag gctgcgtttg ggtggacgag gtggtctgcg
     1021 caatagccgg gaatgacagc atggcggcca gcggcagggc gatgagtagg cggttgttca
     1081 tagaaacctt cctccaagtt ggggagcatg ttttgcttcc cggcacgcct ggggcgtgct
     1141 tgaaatttga atgcgtctca caatcaagta agtaaagcca gcagtttcgc tgtgccggtc
     1201 ggcgctgacg cagcgcctgg cctcaaaacc cgggcgctcg gatgctctgg cgcctgccct
     1261 tcctattaca ggtaagatgc caaaggcagc ctgcctgcag gaggcctaag ttgttgatat
     1321 cgaaagacat tatagcgaag tccaggcccg aaagatagcc tggcagggta attttggcag
     1381 tcctgatata aaaactgcgt gcagacagct ggttaccatc ggttgtcaag tcgcggaaaa
     1441 ctggctactg tttggcggcg ggtctcacag aactgaaaga caaatccggg ggtgaatttg
     1501 ggtacagggc acttggctgc cgcgagcggc tgggggaaaa cgtgggcatc gatcagtgtg
     1561 ctgctgggtg tggctctctt gccgggaaat ggctgggcgg ggggagcaac aacgccgcac
     1621 acgcagatgt cgccgccagg agtagcatcg gcgcgggttc cgctggaacg gctggagctg
     1681 tattccgacc tggccgtcac gtggatgcag gactatttgc gcatcgatac gaccaatcct
     1741 cccggcaatg aaatgcgtgc ggtggctttc tacaaaaaga tcctcgatca ggaagggatc
     1801 gagaatcgca cgttcgagta cgcaccggga cggggcgact tgtgggcgcg tctttcgcgc
     1861 aacgcgccga gcaccggagc cgaagcgaag cggccgatca ttctgctcaa tcacatggac
     1921 gtggttacga gtgacgcatc gcattggaag gtgcctccgt tcagcggcga gatgaaagat
     1981 ggatatctct ggggacgcgg tgcgcaggat atgaaagacg aaggcctggc acagctggcg
     2041 acgatggtga tgttgaagcg ggaaaagatc gcgctggatc gcgacgtcat tttcctggcg
     2101 gtggccgatg aagaagtcga tggaacggga acggattggt tcatcaacca tcagcgcgag
     2161 ctgctggaga atgcggagtt tctgatcaac gagggtgggg agaatctgct ggagaacggc
     2221 cgggtggcat acgtgggcgt ggatgtcggg gagaagacga cctactggct gcacgtggtg
     2281 gcacatggaa cgccggggca tggatcgcgg ccgaatccgg attcagcgcc gaaccggctc
     2341 gtgcgggcgc tcaacaagat cattgcgtat cgcacgccga tccgcgtgtt gccggtggtg
     2401 caagaatttc tgcgcgacat ggcgccctat cagacaccgg agcgggcggc gcaataccgg
     2461 aacataagga aggccgtcga agacaagagg tttgaggagg agatcgagag agacgagtcg
     2521 ctcaatttcc tgctgcgcga cacgatttcg ctgaccatga tgggcggatc ggagcagacg
     2581 aacgtgattc cccccgaggc gtgggcgaat ctggatgtgc gcattctgcc cgg
//
LOCUS       DASPUL010000496         4046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_91956, whole genome shotgun sequence.
ACCESSION   DASPUL010000496 DASPUL010000000
VERSION     DASPUL010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91956"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1925
                     /locus_tag="VEJ00_11765"
     CDS             <1..1925
                     /locus_tag="VEJ00_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01887.1"
                     /translation="NLQPLPPASKFNLSYRYYETEAYVQDSWRARNDLTLTYGLRYQY
                     YSVPYEVSGLEAIANQGFNQYFQPRVQAGLQGSTGPFAPVSFSLAGKANHQPGYYHPD
                     WKDFAPRFSVAYNPSFTSGLLGKVFGDRKTVVRAGAGVVFDHTITSAIANFQQQGSGI
                     FQNSLGTSFGDLATDPRFVAENQLPAGTQQPTPPTNPFTPFFFNGVPVGALTFAQGYG
                     FDTNLKTPYSETITFGIQRELPGNFQFDATYVGRFGRRLLAQTDAAQILDFKDPASGQ
                     TLNQAFTILEGEVRSGAQTFAPLPFFENQFTGGTAACQNNFGVNCTTVTALSNLTGLQ
                     FGAIESAVLTEAQGGELAALGFGGTGLNIGVGMPPQYFFNAYLTNKGASDYNGLLASL
                     HKKISHGLQFDFNYTYSHSLDNSSATANNFAGETQNFTGGLICDATNPRICRGNSEFD
                     ITHVISADGVYDLPFGRGRQFASNVPTWLNLIAGGWQLAGINSWQTGFAFSAISAVSV
                     LSAGENVPPPFTGSTSALRTHIHNSAGTIQLFADPTAAAAAFSAPTGLQGGASRDNLR
                     GPHFWNVDLSLNKHFPIRERMQLEFRAEAYNLFNHANFDLPGLGTADITNPAQFGVIT
                     GTADPRVMQLSLRLEF"
     gene            complement(2018..2944)
                     /locus_tag="VEJ00_11770"
     CDS             complement(2018..2944)
                     /locus_tag="VEJ00_11770"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXX01888.1"
                     /translation="MPARNDVRLEIRHLRLLTAVAEEGSVTKAGRRLHLTQSALSHQL
                     RDAEEKLGVALFLRLGRKMLLTPAGEKMLDSARRVLAELVSAEAQVEDLNGGTHGVIR
                     LSTECYTCYHWLPPVLTKFQAKFPRVEINIVLEATAHPVAALLEGALDVAVVSGPPRN
                     RNLLLTPTCEDELVVVMSPKHRLAATPHVQPRDLAGETIFCYPPKDECTLYQKVLRPI
                     GVTPGRIQEIPLTESMLDMAAAGVGVAFLARWAVAAYASTGKIVTRPLSKGGFRRKWY
                     AATLRTRPAPPYVTEFITLLARTCAMDAVQAL"
     gene            3372..3797
                     /locus_tag="VEJ00_11775"
     CDS             3372..3797
                     /locus_tag="VEJ00_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01889.1"
                     /translation="MNRFARVVAGLSITIVLLSVSAAAQYARRVIRVQIPFEFTVSGR
                     AMPAGDYTIVCLAPDRLELRDTQSHAFAWALTLPGESRETNVSTTLEFSTVDGEHALT
                     ALRVQNERTAYELAARPSPVAVAQRQAHDLIHSAGAGNK"
     gene            3851..>4046
                     /locus_tag="VEJ00_11780"
     CDS             3851..>4046
                     /locus_tag="VEJ00_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01890.1"
                     /translation="MSIALGEKETVESNGATAHVLEVVRSAEQELGGLLQRRLEIVAR
                     INALRQLMNDMVTLFGESILD"
BASE COUNT          843 a         1159 c         1234 g          810 t
ORIGIN      
        1 acaatctgca gccgttgccg ccggccagca agttcaatct cagctaccgc tactatgaga
       61 ccgaagctta cgtgcaagat tcgtggcggg cgcgaaacga tctcacactg acttacgggt
      121 tacggtacca gtactactcg gtgccgtatg aggtaagtgg acttgaagcg atcgcgaatc
      181 agggcttcaa ccaatacttc cagccccgcg tgcaggccgg tttgcagggc tcgacgggtc
      241 catttgcgcc ggtaagcttc tcgcttgctg ggaaagctaa ccaccagccc ggctactatc
      301 accccgattg gaaggatttc gctccacgtt tctcggtggc ctacaatccg tctttcacga
      361 gcggtttgtt ggggaaggtc tttggagacc gaaagaccgt ggtccgcgca ggcgcgggcg
      421 tggtcttcga tcacacgatt acgtcggcga tcgcaaattt ccagcagcaa ggttcgggta
      481 tcttccaaaa ctcactaggc accagcttcg gggatctggc gacagacccg cgctttgtag
      541 ccgagaatca actgccggcg gggacgcagc agccgacgcc gccgacgaat ccctttacgc
      601 cgttcttctt caacggtgta ccagtgggcg cgttgacgtt cgcccagggg tacggattcg
      661 ataccaacct caagacgccg tattccgaga ccattacgtt cggcattcaa cgcgaactgc
      721 cggggaattt ccagttcgat gcgacgtacg ttggccgatt cgggcgacgc ctgctggccc
      781 aaaccgatgc cgctcaaatt ctagacttca aggatccggc ttccggacag acgctgaatc
      841 aggcgttcac aatcctcgaa ggcgaagtca ggagcggcgc ccagactttc gcaccactgc
      901 cgttcttcga gaatcaattc accggtggca cagccgcgtg ccagaataac tttggtgtta
      961 actgcaccac agtcaccgcc ctcagcaact tgacgggtct gcaattcgga gctatcgaaa
     1021 gcgctgtact aacggaagcg cagggcgggg aactggcagc gttgggtttc gggggcacag
     1081 gtttgaatat tggggtcggg atgccgccgc agtatttctt caacgcttac ctgaccaaca
     1141 agggagcttc ggactacaac ggactgctgg cttcgctaca caagaagatc tctcacggcc
     1201 tgcagttcga cttcaactac acgtattccc actccctcga caacagttct gcaacagcca
     1261 acaatttcgc aggggagacg cagaacttca cgggaggttt gatctgcgac gccaccaacc
     1321 cccgtatatg ccgcggaaat tcggagttcg atattacgca cgtgatcagc gctgacggcg
     1381 tttacgatct gccatttgga cgcggacggc agtttgcttc caacgttccg acctggctga
     1441 atctgattgc cgggggatgg cagttggccg gaatcaactc ctggcaaacc gggttcgcgt
     1501 tcagcgcgat ttccgccgtg agcgtactca gcgcggggga gaacgtgcca cctccgttca
     1561 cgggaagcac gtctgccctg agaactcaca tccacaattc agctgggacg attcagttgt
     1621 ttgccgaccc aactgctgct gcggctgcct tcagcgcgcc gaccggtctt caggggggcg
     1681 caagccgcga caacctgcgc ggccctcact tctggaacgt ggatttgtca ctcaacaaac
     1741 atttcccaat acgcgagaga atgcagctcg aattccgagc tgaagcgtac aacctgttca
     1801 accacgctaa tttcgatcta cctggattgg ggacggccga tatcaccaac ccggctcaat
     1861 tcggagtcat cacaggtaca gccgacccgc gcgtgatgca gctatcgctg cggctcgagt
     1921 tctagtctgc agtggaaaaa atcaaaggcc ggcccgaagg ggtcggccct tttttgttgc
     1981 aatagtgttt cgtgaaagag agcagttctg gtaggcctca cagcgcctgc accgcatcca
     2041 tggcgcaggt gcgggcgagc agggtgatga actcggtgac gtagggtggg gcggggcggg
     2101 tgcgcagggt ggcggcgtac catttgcggc ggaatccgcc cttgcttaag gggcgggtca
     2161 cgatcttgcc ggtggaggca taggcagcta ccgcccagcg cgccagaaag gcgacgccca
     2221 cgccggcggc ggccatgtcg agcatggact cggtcagagg aatctcctgg atgcgtccgg
     2281 gcgtgacgcc gatggggcgc aggactttct ggtatagcgt gcactcgtct ttcggcggat
     2341 aacagaagat cgtttcgccg gccagatccc ggggctggac gtgcggcgtc gcggccagcc
     2401 ggtgttttgg actcatcacc acgaccaact cgtcctcgca ggttggcgtg agcagcagat
     2461 tccggttgcg cggagggccg ctcacgacgg caacatccag cgcgccttcg agcagagcgg
     2521 cgacgggatg cgccgtggcc tccagaacaa tgttgatctc aacgcgcgga aacttcgcct
     2581 gaaacttggt caacaccggc ggcagccagt gatagcaggt atagcactcg gtgctgaggc
     2641 ggatcacgcc gtgcgtgcct ccgttcaggt cttcgacctg ggcctccgcg ctgaccagct
     2701 cggcgagaac gcggcgagct gagtccagca tcttctctcc cgccggcgtg agcagcatct
     2761 ttctgcccag gcgcaagaac aaagctaccc caagtttttc ctcggcgtcg cgcagctgat
     2821 ggctgagggc ggactgcgtc aggtgcagtc gcctgcccgc cttggttacg ctgccttctt
     2881 cggccaccgc ggtcaacagg cgcaagtggc ggatttcgag cctcacgtcg tttcttgccg
     2941 gcatcggaac ggtctccttc ccgctgttct cctgactgtt tcgtctgcat gccatttcgc
     3001 caatggactg cattgatggc ggtaaatatg aaatcggctc atgcgtgcaa tgagaacaaa
     3061 caatgatcgt tgtgccacga gctgcgtcca atgaagaaag cgcgctctgt gacattgatc
     3121 gcaagaagcg gatagcgtcg caaaaaaagt ttgcccaaag taatgcaagc aagggcggcg
     3181 caacgcgggg acggatcggc caaaacagag gggcagattg gccagagtcg tcggaggttt
     3241 ggcgacaaag gcgaagacgc gacgcttatg atccccgcgg tcggcgcgaa gacgatagga
     3301 agatctggac gccagaaacg ctgaattcag gatgcaatcg ctacaagatg gaatcgctaa
     3361 aggagaaaac tatgaatcgt tttgcccgag ttgtcgcagg tttatcaatc actatcgttt
     3421 tgctctcggt atccgccgcg gcgcagtatg cgcggcgtgt catccgggtg cagattcctt
     3481 ttgaattcac cgtcagtggc agagccatgc cagccgggga ctacaccatc gtatgtctgg
     3541 cgcccgatcg gctggaattg cgcgacacgc agtcgcacgc gttcgcctgg gcgcttacgc
     3601 tcccgggcga atcccgggaa acgaacgttt cgacgacgct tgagttctcg accgtggatg
     3661 gagagcacgc gctgacagcg ctgcgggtac agaacgagag gacggcttac gaattggccg
     3721 cgcgaccgtc gcccgttgcc gttgcgcagc ggcaagcgca tgacctgatt cactctgcag
     3781 gtgcggggaa caagtagttg acgacgggtt gcacgctacg tccgcgagac gtgcggggaa
     3841 ggagggcgca gtgagcattg ctttgggaga aaaagagact gtggaaagca atggtgcgac
     3901 ggcgcatgtc ctggaggtgg tccgctcagc agaacaggag ttgggcgggc ttcttcagcg
     3961 gcggctcgag attgtggcgc ggatcaatgc cctccggcaa ctcatgaacg acatggtgac
     4021 gctgttcggc gagtcgattc ttgacg
//
LOCUS       DASPUL010000497         8126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1868444, whole genome shotgun sequence.
ACCESSION   DASPUL010000497 DASPUL010000000
VERSION     DASPUL010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868444"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VEJ00_11785"
     CDS             complement(<1..777)
                     /locus_tag="VEJ00_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01891.1"
                     /translation="MRIRRAYPITLAVVIVAAAVTVAVQLRKNAPPEPARLLPGADAF
                     FYADFGWVRKILTKPLPAVSHDPEYERFIQESGIDFERDLDAVAFAVHYPTNQPVHAE
                     GEPRFSEVLVGKFDGQKLAEYLKHVAQSVESYNSVEIYSIPVEDRTFRVAILGVDSVA
                     ASNHDDQAVIRGIVDRSRRLASPFGGPALLRHYYKQVQLASPIWLVGRIDPGSPEFGG
                     WSDFLAKPADVVISASYSPFHLPLRTSVLHVRAEAWTASAD"
     gene            1042..3528
                     /locus_tag="VEJ00_11790"
     CDS             1042..3528
                     /locus_tag="VEJ00_11790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX01892.1"
                     /translation="MLIADLHIAIRQLRKSPGFAATAVLMLALGIGATTAIFSIVEAV
                     LLRPLPFPQSGQLVSLSDILQGTSIGGNSRPRVTAPDVRAYTRDTHSFESLGGYSQSV
                     NVELSGIGEPAQVYASRLTGSVFPTLQVAPLVGRVFTQQDDDQNQQVAVVSYGMWQSR
                     LHGDPHVLGTKILLDRKPYLVIGVMPRDFDFPLLPGRLDHSELWVPMSFEPQELSGAG
                     QANWTFEMVGRLKPGVTAEQAQQDAQRVAEETMRAYPPFMANLHIQALVRSLREQTVS
                     QARDLVRILFLATVVVLLIVCANLAGLLLVRAIRRRREIAMRMALGAGKLALLRQSVL
                     ESLMLSVSGGMLGLVFATIGMQAGLSRLPETLPRVNEIGLDWQVAAFAFAISVLTGIG
                     CGLAPAFAAWHTSVSETLKEGGRTGSAGGHAWLRSSLVIGEIAVALVLLAACGLLLRS
                     FEKMRAVDLGFRADHVLSASYALPEKQYPTQNSVNEFNRELILHAQQLPGVKAAGLTS
                     FLPTTGAWSGGGFVVEGFTPHEGSAMRFSTEVQIEGDFFRAMGISLLAGRFFTLADNA
                     DSQLVTIVNRKLAEHYWPGADPIGKRLRVGTPEAPTPWLTVVGEVADINEDSPDIPGK
                     EQLYQPIEQYAKSLGSLASPSILIGNSGFVTLRTETDPVQSANTLRVAVRSIDPQLPL
                     TQVQSMESVVSDSEAPRRFNTALISSFAIAAVLLAALGIYSVVAFSAALRVQEMAIRM
                     ALGSQRSGILGLVFLSAIKLALAGCALGLAGVAATAHLLDSLLFSVSRFDPLVLAIAA
                     AFVLALAVAASVLPAARAAATDPMQALRAE"
     gene            complement(3536..4171)
                     /locus_tag="VEJ00_11795"
     CDS             complement(3536..4171)
                     /locus_tag="VEJ00_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01893.1"
                     /translation="MPSSRAQAVIAHIVLCILASAAQAQTKSKPLFSEPLSLCSGHGF
                     VAMAPFSWSESFVVVMIDEKGIEAPKTIQTPNNGIIGLQCLGSHLELHVREDQSDHSS
                     VLPYAIRDGKIEPEPREDINWSISGKGPMPSAVERRSDGFGASAANAATRGDWYELVC
                     IGVNHIYELHRVFHEIDGVSKFRAELVEETFDHKVTKALPLVYYERAEHGD"
     gene            4373..4448
                     /locus_tag="VEJ00_11800"
     tRNA            4373..4448
                     /locus_tag="VEJ00_11800"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4406..4408,aa:Thr,seq:tgt)
     gene            4721..4809
                     /locus_tag="VEJ00_11805"
     tRNA            4721..4809
                     /locus_tag="VEJ00_11805"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4755..4757,aa:Tyr,seq:gta)
     gene            4818..4997
                     /locus_tag="VEJ00_11810"
     CDS             4818..4997
                     /locus_tag="VEJ00_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01894.1"
                     /translation="MNRLAAALIAYAVLAVLTWMTISDPRIRVFPLGILALFAMKSIL
                     RRNDVLHPGKGDKTE"
     gene            5097..5172
                     /locus_tag="VEJ00_11815"
     tRNA            5097..5172
                     /locus_tag="VEJ00_11815"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5130..5132,aa:Thr,seq:ggt)
     gene            5313..5387
                     /locus_tag="VEJ00_11820"
     tRNA            5313..5387
                     /locus_tag="VEJ00_11820"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5345..5347,aa:Gly,seq:tcc)
     gene            5412..5741
                     /locus_tag="VEJ00_11825"
     CDS             5412..5741
                     /locus_tag="VEJ00_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01895.1"
                     /translation="MAFEQAAIKIEKAQEFSRLQAAVEQAFLAEKVGRFLKQLDRKGV
                     RIRDFEAVLAERALESVGGSELNARQLYDSLPVSDRAQMREFYLSQLESVDVALRHRF
                     KKLYQYY"
     gene            5897..7084
                     /gene="tuf"
                     /locus_tag="VEJ00_11830"
     CDS             5897..7084
                     /gene="tuf"
                     /locus_tag="VEJ00_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HXX01896.1"
                     /translation="MAKEKFERTKPHCNVGTIGHIDHGKTTLTAAITKVLAKNNPKIV
                     FRSFDSIDNAPEEKARGITIATAHVEYETANRHYAHVDCPGHADYIKNMITGAAQMDG
                     AILVVAATDGPMPQTREHVLLARQVGVPYIVVALNKCDAVDDPELLDLVELEVRELLK
                     SYQFPGDDVPVVRLSALGALNGEAKWEKQIDELMAAVDKYVPQPQRELDKPFLMPIED
                     IFSISGRGTVVTGRIERGKVKVGEEVEIVGFRETRKTVVTGVEMFKKQLDEGMAGDNA
                     GLLLRGTGKDEVERGMVLAKPASITPHTKFKAEVYILTKEEGGRHTPFFKGYRPQFYL
                     RTTDVTGVAELPSGTEMVMPGDNVSLVIELITPVAMEKGLRFAIREGGHTVGAGTIAE
                     IIQ"
     gene            7187..7339
                     /gene="rpmG"
                     /locus_tag="VEJ00_11835"
     CDS             7187..7339
                     /gene="rpmG"
                     /locus_tag="VEJ00_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005918839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXX01897.1"
                     /translation="MPREIVTLQCGDCKERNYSTTKNRKTTTDRLEFKKFCPRCRKHT
                     AHKEVK"
     gene            7411..7486
                     /locus_tag="VEJ00_11840"
     tRNA            7411..7486
                     /locus_tag="VEJ00_11840"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7445..7447,aa:Trp,seq:cca)
     gene            7534..7782
                     /gene="secE"
                     /locus_tag="VEJ00_11845"
     CDS             7534..7782
                     /gene="secE"
                     /locus_tag="VEJ00_11845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HXX01898.1"
                     /translation="MASIAEENAIVTTVKSWPDRIRSFYNDVRTEMRKVTAPSWKEVQ
                     GTTTVVVITVAIFALYFWVIDFFIQRGVTYMFNYFKAH"
     gene            7804..>8126
                     /locus_tag="VEJ00_11850"
     CDS             7804..>8126
                     /locus_tag="VEJ00_11850"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HXX01899.1"
                     /translation="MVDEEKKEAETSTAAEPQAAGSDTTAVAAEAPRNPNMKWYIIHS
                     YSGFERKVKESLESRVVAFGLQDKIGKVLIPTESVTEVRGGKKYTSERMFYPGYVLVE
                     MDMDD"
BASE COUNT         1622 a         2357 c         2540 g         1607 t
ORIGIN      
        1 gtcggcgctg gccgtccagg cctcggctcg cacgtgaagg acgctggtgc gcagaggaag
       61 atggaacgga ctgtagctgg cggagatgac cacatcggcc ggcttcgcga ggaagtcgga
      121 ccatccgccg aattccggcg agcccgggtc aatgcgccct accagccaaa tagggctggc
      181 cagttgcacc tgcttatagt agtggcgcag gagagccggt ccaccaaagg gcgatgccag
      241 ccgccgcgag cggtcgacga taccccgaat cacggcctga tcgtcgtggt tcgaggctgc
      301 caccgaatcc acccccagga tcgccacccg gaacgtccgg tcctccacag gaatcgagta
      361 gatctcgacc gagttatagc tttcgaccga ctgtgcaacg tgcttcagat actcagccag
      421 cttctgcccg tcgaactttc ccaccagtac ttccgaaaaa cgcggctcgc cttcggcatg
      481 gaccggctgg ttcgttgggt agtgaaccgc aaaggccaca gcgtcgaggt cacgttcaaa
      541 atcgatgcct gactcctgaa taaaccgctc gtattcgggg tcgtgggaga cagccgggag
      601 gggcttggtg agaatcttgc gaacccagcc aaagtccgcg tagaagaagg cgtcggcccc
      661 gggcagcagg cgcgccggct caggcggtgc gttcttgcgc agctgaacgg cgaccgtaac
      721 cgccgccgcc acgatcacga ccgccagcgt aatcggataa gcgcgtcgga ttcgcatgga
      781 atgcggtggc aggaatcata gcacccggcg gtggatatcc cctgtgctcg ggcggcgcgg
      841 acgtcgatcg gcccggccgg cctccctcgc cccgactgtc cggtctcgaa caggatttcg
      901 cgaatctgga cggaaggcac cataagaaat tgccgcagga cgcctaacta ttaatggagg
      961 aaccacttag acaagatcgc tttggctcga gaaatgctca atcctcgcct tcggatcttg
     1021 tcttcgatgg gaatgccaca aatgctgatc gctgacctgc acatcgcgat tcgccaactg
     1081 cgaaagtcgc cggggtttgc agcgacagcc gtgttgatgc tggccctcgg catcggcgcc
     1141 accaccgcga tcttctcgat tgtcgaggcc gtcctgttgc ggccactgcc ctttccccaa
     1201 tctgggcaac tggtctccct ctccgacatt ttgcagggca ccagcatcgg gggtaacagc
     1261 cgccctcgcg ttaccgctcc cgatgtccgc gcctacacgc gcgacaccca cagcttcgag
     1321 agcctgggcg gatatagtca gtcagtcaac gtcgaactct cgggcatcgg cgagccagcg
     1381 caggtatatg catcccgctt gactggcagt gtgttcccaa cgttgcaggt ggcgccgctc
     1441 gtgggccgag tctttacgca acaggacgac gatcagaacc agcaggtggc ggtggtcagt
     1501 tacgggatgt ggcagagccg cctgcacggc gatccgcatg tgctgggaac gaagattctg
     1561 ctcgaccgca aaccgtatct cgtcatcggc gtcatgcccc gcgacttcga tttcccgcta
     1621 cttccgggac gcctggatca cagcgaactg tgggttccca tgagctttga gccgcaggaa
     1681 ttgagcggcg ccggacaagc aaactggacc tttgaaatgg tgggccggct aaagcccggc
     1741 gtcaccgccg aacaagcgca gcaggacgcg cagcgcgtgg ccgaagaaac catgcgcgcg
     1801 tatccgccgt tcatggcgaa tttgcacatt caagccttgg tccggtcgct gcgcgagcaa
     1861 accgtctcgc aggcgcgtga cctggtacgc attctgtttc ttgccaccgt agttgtgctg
     1921 ctgattgtct gcgccaacct tgccggattg ttgctggtcc gtgccattcg ccgccgccgc
     1981 gaaatcgcca tgcgtatggc gttaggcgcc ggcaaacttg cactgctccg ccaatccgtg
     2041 ctcgagagcc tgatgttaag cgtcagcgga ggcatgctcg gtctggtctt cgccacgata
     2101 ggcatgcaag cgggccttag ccggcttccc gagactttgc ctcgcgtgaa cgaaattggc
     2161 ctcgattggc aggttgcggc attcgccttc gcgatatctg ttctcaccgg catcggctgc
     2221 ggattggccc cggctttcgc cgcctggcac accagcgtca gcgagacttt gaaagagggc
     2281 ggccgcactg gctccgccgg aggccatgcc tggctgcgct cgtcattggt cataggcgaa
     2341 atcgccgtcg cgctggtgtt gctggcagcc tgcggcctgc tgctgcgaag tttcgagaaa
     2401 atgcgcgccg tcgatctcgg cttccgcgcg gatcatgtac tttccgcatc ctacgcgctg
     2461 ccggagaagc aatatcctac gcagaactcg gtcaacgaat ttaatcgcga gttgattctc
     2521 cacgcgcagc agctacccgg cgtgaaggcg gccggtttga cttcctttct ccccacgact
     2581 ggcgcctgga gcggaggtgg attcgtcgtc gagggattca ctccgcacga gggctccgcg
     2641 atgaggttct ctaccgaggt gcagatcgag ggcgatttct ttcgcgcgat gggcatttcg
     2701 ctgcttgcgg gcaggttctt cacgctcgcg gacaatgccg acagccaact cgttaccatc
     2761 gtcaaccgca agctggccga gcactactgg cccggtgccg atcccatcgg caagcgcttg
     2821 cgcgtcggta cgcccgaagc tcccactccc tggctcaccg ttgtgggcga agttgccgac
     2881 atcaatgaag actcgcccga tattcccggc aaagagcagc tctatcagcc gatcgagcaa
     2941 tacgcgaaat ctctgggctc gctcgcctca ccgtccatat tgatcggcaa ctcaggcttc
     3001 gtcacgctgc gcacagaaac cgatcccgtg cagtcggcca acaccttgcg cgtcgcggtc
     3061 cgctccatcg acccgcaact cccgctcacg caagtgcaaa gcatggagag cgttgtctcc
     3121 gacagcgaag ccccgcgccg tttcaacacc gccctaatct ccagcttcgc gatcgctgcg
     3181 gtgctgctgg cggcgcttgg aatctatagc gtcgtcgcct tctcggctgc actccgcgtt
     3241 caggagatgg cgatccgcat ggccttggga tcgcaaaggt cgggaattct gggcctggtg
     3301 tttttgtcgg cgatcaaact cgcccttgcc ggttgtgcct taggattggc cggcgtggcc
     3361 gcaaccgctc atctgctcga ttcgttgctg ttctccgtga gccgcttcga tcctctggtg
     3421 ctcgccatcg ccgccgcttt cgtgctggcg ctggcggttg cggcgtcggt gctccccgcc
     3481 gctcgagccg cagctaccga ccccatgcag gcgctccggg cagagtgaac agggcctagt
     3541 ccccgtgctc ggccctttca tagtagacga gaggcaatgc cttcgtgacc ttatgatcaa
     3601 aggtttcctc cacgagctca gcccgaaatt tggaaacgcc gtcaatctca tggaagactc
     3661 gatggagttc ataaatatgg tttaccccaa tacaaaccaa ttcataccaa tccccgcgcg
     3721 tcgcagcatt ggcggcgctt gcgccgaatc cgtcactcct gcgctcaacc gctgacggca
     3781 taggcccctt accagatatg gaccaattaa tatcctcccg tggttccggt tctattttgc
     3841 cgtccctaat cgcgtatggc agcacggagg aatgatccga ctggtcttcc cggacatgca
     3901 actctaagtg tgaacccaag cattgcaacc cgataatgcc gttgttcggt gtctggatag
     3961 ttttcggggc ctcaatccct ttttcatcga tcatcacgac gacgaacgat tcagaccagc
     4021 tgaatggggc catagctaca aatccgtgtc cagagcacaa cgacaacggc tccgaaaaca
     4081 ggggcttgct tttggtctgc gcctgagctg cgctggctaa tatgcaaagg acgatgtgcg
     4141 cgatcacggc ctgggcacgg gacgatggca tggtgggatg gtggcctccc tctgcgtgat
     4201 gactgcgatt gtgtcatcat cacgggctcg cgtcgattgt ccttggtaac ccgagctcga
     4261 cgttgagttt gcaggcccaa tcccgattgt gctagtttat ataattgtcc cggacatctg
     4321 ggcgggatcc gtgggataag tttgcgcgga ggcgcgcgct gtccccagac gcgctggcgt
     4381 agctcagtcg gtagagcatc tgatttgtaa tcagagggtc gggggttcga atcccttcgc
     4441 cagctccagg aaacgcagaa gtttagcacg aagacgcaag ttgtaacggc aggcggcgga
     4501 ctcggtagcg gcgcaacagg attttccaaa cgcactccat tccgaacttt ttccggcttg
     4561 gtggtattcg cggcgggttg gtcgcgagcc ttggtttccc tgtttggcga aaagcaaagt
     4621 aacgtgcgcg gcgggctgat tgcgggtttt gctgtgcgct ctggcagctg gcagcgtggt
     4681 ttgccgggcg gacggcgggg cgcatcgccc gagattttct gcacaggtgg gtgagtggtt
     4741 aaagccgaca gactgtaaat ctgttcctcc tagcgaggta cggaggttcg aatcctcccc
     4801 tgtgcaccag gattaacgtg aatcgcctag cggcagcact gatcgcgtac gctgtacttg
     4861 ccgtgctgac ctggatgacg atcagcgatc cgcggattcg ggtcttcccg ctgggaattc
     4921 tagctctgtt cgccatgaag tcgattctgc gacggaacga cgtgcttcat cccggcaagg
     4981 gcgacaagac ggagtagggt ttgactgagg gttgacggct gtttttcgtg gaagggcacg
     5041 gcttcagccg tgccgtcgag agtcttacga gagacggctt tagccgctgg gttcgagccg
     5101 atgtagctca gttggtagag cactcccttg gtaagggaga ggtcaccagt tcaatcctgg
     5161 tcatcggctc cagattctgc tcgatgcgct gccgggaagg cgctttgaaa gggcgcggtt
     5221 ttagccgcgc cgcagcgatt gaaatggaga agcggcttta gccgccgggt ttgctgaagg
     5281 ctgacagccg atggctgaca gctggtttca aggcgggagt aactcagtgg tagagtcaca
     5341 gccttccaag ctgttggtcg cgggttcgat tcccgtctcc cgctccagag tttaattgct
     5401 tggtttggac catggctttc gagcaggcgg cgatcaagat tgagaaggcg caggagttct
     5461 cgaggctgca agccgcagtc gagcaggcat tcctggccga gaaagtcggg cgcttcctga
     5521 agcaactgga tcgcaaggga gtccgcatcc gggattttga agcggtgctg gccgagcggg
     5581 cgctggaaag cgtcggcggg tcggagttga acgcacggca actatatgac tcgctgcccg
     5641 tctccgaccg ggcgcagatg cgcgagtttt atctgtcgca gctggagagc gtcgacgttg
     5701 cactacgcca caggtttaag aagctgtacc agtattactg atcgcggttt gcgggcgtga
     5761 ggagcgaagc gacgggagcc cgcagcgatc atcctgagcg aagcgaagga tcttggtttc
     5821 gtatcagggc gcggcttgag ccgcgccgta tcgggcaatc ataaggtttt ggggctttag
     5881 cccctgagga acggaaatgg cgaaggaaaa atttgaacgc accaagccgc actgcaacgt
     5941 ggggacgatc gggcacatcg atcacggcaa gaccacgttg acggcggcga tcacgaaagt
     6001 attggcgaag aacaatccga agattgtgtt ccggtcgttt gattcgatcg acaacgcgcc
     6061 ggaagagaaa gcccgcggca tcaccatcgc gacggcgcac gtggagtacg agacggcgaa
     6121 ccggcactac gcgcacgtgg attgtccggg gcacgccgac tacatcaaga acatgatcac
     6181 gggcgcggcg cagatggacg gcgcgattct ggtggtagca gcgaccgacg gtccgatgcc
     6241 gcagacgcgc gagcacgtgc tcttggcccg ccaggtaggc gtgccctaca tcgtggtggc
     6301 gctgaacaag tgcgatgcgg tggatgatcc cgaactgctc gacctggtcg aactggaagt
     6361 cagagagctt ttgaagtcgt atcagttccc gggcgacgac gtgccggtgg ttcggctctc
     6421 ggcgctgggc gcgctgaacg gcgaggcgaa gtgggagaag cagattgacg agctgatggc
     6481 ggcggtggac aagtacgtgc cgcagccgca gcgtgagctg gacaagccgt tcctgatgcc
     6541 gatcgaggat atcttctcga tctcgggccg cggaaccgtt gtcactggcc gcatcgagcg
     6601 cgggaaggtc aaggtgggcg aggaagtgga gattgtcggg ttccgcgaaa ccagaaagac
     6661 ggtggtgacg ggcgtcgaaa tgttcaagaa gcagctggac gaaggcatgg cgggcgacaa
     6721 cgccggcctg ttgctgcgcg gcacaggcaa ggacgaagtg gagcgcggga tggtgctggc
     6781 gaagccggcg tcgatcacgc cgcacaccaa gttcaaggcg gaggtgtaca tcctgacgaa
     6841 ggaagagggc ggacgtcata cgccgttttt caagggatac cggccgcagt tttacctgcg
     6901 gacgacggac gtgacgggag tcgccgagtt gccgagcggc acggagatgg tgatgcccgg
     6961 cgacaacgtg agcctggtga tcgaactgat cacgccggtg gcgatggaga aggggttgcg
     7021 gttcgcgatc cgcgaaggcg gccacacggt gggcgcgggc acgatcgccg agattattca
     7081 gtaggtaatt gtggcgcggg cgccctcgcc cgcgatcgca tgtagtggcc ggatcgaccg
     7141 cgcgggcgag ggcgcccgcg ccacatagct aggttgggag tgagcaatgc cccgagaaat
     7201 cgtgacgtta cagtgcggtg attgcaaaga gcgcaattac tcgacgacca agaatcgcaa
     7261 gacgacgacg gaccggttgg aattcaagaa attctgcccc cgttgccgca agcacaccgc
     7321 tcacaaggaa gtgaaatagt cgcaagttgc aagtccccgg ttgcaagccc cttcgctcgg
     7381 gacttgggac tggtagctcg ggactgatgc aggggcgtaa gctcaacggt taaactgccg
     7441 gtctccaaaa ccggacttgg gggttcgaat ccctccgccc ctgccagaat tttgcggccc
     7501 ggtgagggaa cggacccaag aggaagtacg aagatggcga gcatcgcaga agagaacgca
     7561 atcgttacta cggtgaagtc ctggccggat cgcatccggt ctttctacaa cgacgtgcgc
     7621 accgaaatgc gcaaggtcac cgcgccgtcc tggaaagaag tccaaggcac aaccacggtg
     7681 gtcgtcatta cggtagcgat ctttgccctt tacttctggg tgatcgactt cttcatccag
     7741 cggggcgtga cgtacatgtt caactatttc aaggcacatt gagttttcca aaagaagcac
     7801 cctatggtgg acgaagagaa aaaagaagcg gaaaccagca cagcggccga accgcaagcc
     7861 gcaggctccg acaccaccgc cgtcgctgcc gaggcgccac gcaatccgaa catgaagtgg
     7921 tacatcatcc actcctattc gggatttgag cgcaaggtca aggagtcgct cgagtcgcgc
     7981 gtcgtcgcct tcggactgca ggacaagatc ggcaaagtcc tgatcccgac cgagtcggtc
     8041 accgaagtgc gcggcggcaa gaaatacacc tccgagcgca tgttctatcc cggctacgtg
     8101 ctggtcgaga tggacatgga cgacca
//
LOCUS       DASPUL010000498         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1869020, whole genome shotgun sequence.
ACCESSION   DASPUL010000498 DASPUL010000000
VERSION     DASPUL010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869020"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VEJ00_11855"
     CDS             complement(<1..778)
                     /locus_tag="VEJ00_11855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXX01900.1"
                     /translation="MATATAAATSFEKPTSTISRVLYPTALLAALSMWLAAMQAPLWV
                     DETLVYWQMSGGLARIWSRSAQMPSSFAYLYILWFAKSVLGSREIALRIPSVLAMLAA
                     AYFLYRVARELFGTEIAWITCILFCAHKDIVLAAGNARPYAFALLMTNLAIFSLVRWT
                     ARNEMRFAILAGAAAAGILYFHFLFGAILPAFAIYFLALRGRSVKASAAQLAAMLASF
                     VLVGAPLAPRFINLFQAKQPHILETVPETLRVLQTLVPFNL"
     gene            complement(1002..>2461)
                     /gene="pilQ"
                     /locus_tag="VEJ00_11860"
     CDS             complement(1002..>2461)
                     /gene="pilQ"
                     /locus_tag="VEJ00_11860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HXX01901.1"
                     /translation="PVRAAEAAAKFVEKPEGNLLPTPSAAPQTSQTPSVTAMQPAVNL
                     AAEQKQQMSQAAPAAAEAKYTGEPISVNLKDVDLKDFFRLIHEISGLNVVLDPNVKGT
                     LTIVLDDVPWDQALDIVLKNNDLARQLDGNVLRIATVDTLRHEADARRAQHDAEALAV
                     DKVTFTRFLSYAHARDLITTCKKFISQRGDVIADERTNAIIVNDVPSVIPVIDRLLTQ
                     LDRKTQQVEIEARVVAATRSFARDIGTQLGFGWGNHTTNVGGAGSVGTSPAGVFGLQP
                     LYPIIGTSAPTAGTLTQIPLFSNLPSTSPTSGITFQNFSRNIQLDAILTMAESRGLVK
                     ILSRPRVVTQNNIQALVRQGVRVPIVTQAQLGGPPTVTYVDAFLRLTVIPQITSEGTI
                     FLNVDVENTTPDFGESIQGNPTLITQQATTQVLVTDGGTVVIGGVIQTNNSVQISQVP
                     LLGSIPYLGNLFKHTQVSTNNQELIFFITPRIIQT"
BASE COUNT          492 a          726 c          750 g          493 t
ORIGIN      
        1 gcagattgaa gggcacgagt gtctgcagca cccgcagcgt ttccggcacc gtctccagga
       61 tgtgcggctg tttggcttga aacagattta tgaatcgtgg ggcaagtgga gcccccacca
      121 acacaaacga agccaacatg gccgccagct gcgctgcgct ggctttgacc gagcgtcctc
      181 gcagcgccag gaagtaaatc gcgaacgccg gcaggatcgc tccgaacaag aaatggaagt
      241 agagtattcc ggcggcagcg gccccggcca ggatggcaaa acgcatctcg ttccgcgcgg
      301 tccatcgaac gagggaaaaa atcgccaggt tcgtcattag caaagcaaac gcgtacggac
      361 gcgcgttgcc tgccgccagc acaatatctt tatgggcgca gaagagaatg caggtgatcc
      421 atgcgatctc cgtgccgaag agctctcgtg ccacgcgata caggaaatag gcggcagcga
      481 gcatggccag caccgaggga attctcagtg cgatctcccg gctgcccagc accgacttgg
      541 caaaccagag gatgtagaga taagcgaagg acgaaggcat ttgcgccgag cggctccaga
      601 ttctcgccaa gccgccgctc atttgccagt aaaccagggt ctcatccacc caaagcggag
      661 cctgcatcgc cgccagccac atcgacaacg cagccagcaa agccgtggga tagagcactc
      721 gcgagattgt ggaggttggt ttttcgaaac tcgttgccgc cgcggttgcc gtcgccacct
      781 tcgctgcttt cccccgcgtt cgcgagtgtc aagaacggcc tgattgtact gccaacgggt
      841 ttggttcgcg acaccctgag gtaacttatg ccgccgtggg catcagaagc agcacggtcc
      901 cgactgccgt cgggctattt gatttctctg gccaacaaaa acaggaccgc cggagccctc
      961 tcgggctgcc ggcggctgtt ttgctgctaa ctcgattgcg gttatgtctg aatgatccgc
     1021 ggagtgatga agaaaatcag ctcctggttg ttggtgctga cctgcgtatg cttgaacagg
     1081 ttgcccaggt agggaatact gcccagcaac ggtacctggc tgatctgcac cgagttgttg
     1141 gtttgaatca cgccgccaat caccaccgtt ccgccatcgg taaccagtac ctgggtggtg
     1201 gcctgctgcg tgatcagagt cgggttgcct tgaatcgatt ctccaaagtc cggcgtggta
     1261 ttctccacgt ccacgttcag gaagatggtt ccttccgagg tgatctgcgg aattacggtc
     1321 aagcggagga aagcgtcgac gtaggtcacc gtgggcggac cacccaactg cgcctgggtt
     1381 acgatcggta cgcgcacgcc ctggcgaacc agcgcctgta tgttgttctg ggtgaccact
     1441 cgcggacgcg agaggatctt caccagtcca cgcgactccg ccatcgtcag gatcgcgtcc
     1501 agctgaatgt tcctgctgaa gttctgaaag gtgatgccgc tggtcgggct ggtcgagggc
     1561 aggttggaga acaagggaat ttgggtcagc gtgcccgcag tgggtgcgct ggtgccgata
     1621 atgggataga gcggctgcag tccgaatact ccagccggcg acgttcccac gctgccggcg
     1681 ccgcccacat tggtggtgtg gttgccccag ccgaagccca gctgcgtgcc gatgtcgcgg
     1741 gcaaagctgc gggtcgcggc cactacacgg gcttcaatct ccacctgctg tgtcttgcga
     1801 tccaactggg tcagcagccg gtcaatcacc ggaatcaccg aagggacgtc gttcacgatg
     1861 atggcattcg tacgttcatc cgcgatcacg tcaccacgct gcgaaatgaa cttcttgcag
     1921 gtagtgatca ggtccctggc atgggcatag ctgaggaacc gcgtgaacgt gaccttgtca
     1981 actgccagcg cttccgcgtc atgctgtgcc cggcgggcat ccgcttcatg gcgcagcgtg
     2041 tcaacggtgg caatgcgcag gacgttgccg tccagctgac gtgccagatc gttgttcttg
     2101 agcacgatgt cgagggcctg gtcccacggc acatcatcca gcacaatcgt gagcgtgccc
     2161 tttacgttcg ggtccagtac cacgttcaaa ccgctgatct cgtgaatcag gcggaagaag
     2221 tctttcaggt ccacgtcctt caggttcacc gaaataggct cacctgtata cttggcctcg
     2281 gccgcagcag gtgccgcctg cgacatctgc tgtttctgct cagccgccag gttaaccgct
     2341 ggttgcatgg cggtaaccga aggggtctgg gaggtctgcg gcgccgcgct gggcgtcggt
     2401 aacagattgc cttccggctt ctccacgaac ttggcggcag cctccgcggc gcgaaccggc
     2461 g
//
LOCUS       DASPUL010000499         3075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_644977, whole genome shotgun sequence.
ACCESSION   DASPUL010000499 DASPUL010000000
VERSION     DASPUL010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644977"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VEJ00_11865"
     CDS             <1..315
                     /locus_tag="VEJ00_11865"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01902.1"
                     /translation="TVDYGGMSWRGHVGVVTTLFKYARLKPANSVAMVCGPEIMMRFV
                     ARELESHGLGRNDIYLSMERNMKCAVGFCGHCQYGPHFICKDGPVFTYNQMKPLLEKY
                     EL"
     gene            305..1114
                     /locus_tag="VEJ00_11870"
     CDS             305..1114
                     /locus_tag="VEJ00_11870"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01903.1"
                     /translation="MSSETKSKSSSPSKRPRIGVFKFASCDGCQLSLLDAEDELLSVV
                     GAVEIAYFPEASRTMLKGPYDIGLVEGSITTHHDAERIQEVRRKCKTLITIGACATAG
                     GIQALRNWKDVDEFVRVVYASPQFISTLKLSTPIGEHVPVDFELRGCPINKYQLIELI
                     TATLAGRKPNVPTYSVCMECKRKENVCIAVARGVPCLGPVTQAGCGALCPSYDRGCYG
                     CYGPMETPNTASLTSQFRILGQSDEQVSRAFRSFNAWAWPFRKASEECERA"
     gene            1111..2409
                     /locus_tag="VEJ00_11875"
     CDS             1111..2409
                     /locus_tag="VEJ00_11875"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HXX01904.1"
                     /translation="MNGSGSRTIKVDTLARVEGEGALYIKMAGERVTDVKLRIYEPPR
                     LFEAFLRGRHYSEAPDITARICGICPIAYQMSAVHAIERALGIKIDPAVRLLRRLFYC
                     GEWIESHALHVYMLHAPDFHGYPDVIEMAKDHRAVVEKALRLKKIGNSIVHLLGGREI
                     HPISAAVGGFHKTPSKRQLKELISDLEWGLEASLEAVHWTSTLQFPDFEQDYEFVALR
                     HPDEYPFNEGRLVSNRGLDIDAAEYEDRFVELHVKHSNALHSVIRGRGSYLVGPLARF
                     NLNFDKLPEVAQKAALEAGLKPPVKNLFRSITVRAVELVFAFAEALRVIREYEPPSAP
                     RAIVPDRAGVGQAITEAPRGILYHRYAVDEKGLIVTAKIIPPTSQNQRRIEDDLREYA
                     AQLAAWPLEEATWKCEQAVRCYDPCISCATHFLKVEIDRE"
     gene            2460..2897
                     /locus_tag="VEJ00_11880"
     CDS             2460..2897
                     /locus_tag="VEJ00_11880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HXX01905.1"
                     /translation="MLIIGCGNRQRGDDGAGIIAVERLRALGIPAKICTGESSELIDA
                     WSGADDVVVIDAVVTGAPTGTVHVWDGQHPMRPSRPSASTHGIGVAEAIELARTLGRL
                     PPKLRVLGIEGKTFKMGNNISPEVERAVEHVVERIAREVGAAK"
BASE COUNT          622 a          910 c          956 g          587 t
ORIGIN      
        1 accgtggact acggcggcat gagctggcgc gggcacgtgg gcgtggtcac gaccctgttc
       61 aagtacgcgc ggttgaaacc ggcgaactcg gtggcgatgg tgtgcgggcc ggagatcatg
      121 atgaggtttg tcgcccgcga actggagtcg catgggcttg gccgcaatga catctatctt
      181 tcgatggaac gcaacatgaa atgcgccgtc ggcttttgcg ggcactgcca gtacggaccg
      241 cacttcattt gcaaggacgg ccccgttttt acttataacc agatgaagcc gctgctggag
      301 aagtatgagc tctgaaacca aatcgaaatc cagctcaccc tcaaaacgtc cgcggatcgg
      361 cgtgttcaag ttcgcctctt gcgacggctg ccagttgagc ctgctcgatg ctgaagatga
      421 actgctgagt gtggtcggcg ctgtcgagat cgcctacttc cccgaagcca gccgtacgat
      481 gctgaagggg ccttacgaca tcggcttggt ggaaggatcg atcaccacgc accacgatgc
      541 tgagcgtatt caggaagtgc ggcggaagtg taaaacgctg atcacgattg gagcctgtgc
      601 cacggcgggt ggcattcagg cgctgcgcaa ttggaaggac gttgacgagt ttgtccgggt
      661 cgtgtatgcc tcgcctcagt tcatcagcac gctgaagttg tcgactccga tcggcgagca
      721 cgtcccggtg gatttcgaac tgcgcggctg ccccatcaac aagtatcagt taattgagct
      781 catcacggcg acgctggccg gccgcaaacc gaacgtccct acctacagcg tgtgcatgga
      841 gtgcaagcgg aaagaaaacg tctgcattgc ggtagctcgc ggcgtgccgt gcctgggccc
      901 ggtcacgcaa gcaggatgcg gcgctctttg cccgagctat gaccgcggtt gctatggctg
      961 ctacgggccg atggaaacgc cgaacacggc ttccttgacc agccagttcc gcatcctggg
     1021 ccagagcgac gagcaggttt cccgggcgtt tcgcagcttc aacgcctggg cctggccgtt
     1081 ccgcaaggcc agcgaggagt gtgagcgcgc atgaacggga gcggcagcag aacgatcaaa
     1141 gtcgacacgc tggcccgggt ggagggtgaa ggcgcgctgt acatcaaaat ggcgggcgag
     1201 cgcgtgaccg acgtgaaact caggatctac gagccgccgc ggctgtttga ggcattcctg
     1261 cgcggccgtc actattccga agcgcccgat attacggcac gcatctgcgg catctgcccg
     1321 atcgcctacc agatgagcgc cgtacacgcg atcgagcgcg cactgggcat caagatcgac
     1381 cccgctgtcc ggctgctgcg ccgccttttc tactgcggcg aatggatcga aagccacgcc
     1441 ttgcatgtct acatgctgca cgcgccggat tttcacggct atcccgacgt gatcgagatg
     1501 gccaaggatc atcgcgccgt ggtcgagaag gcgcttcgcc tgaagaagat cgggaactcg
     1561 attgtgcact tgctgggcgg ccgcgagatt catcccatct ctgcggccgt cggaggtttc
     1621 cataagacac cctccaagcg acaactgaag gaactgatca gtgatctgga gtggggactc
     1681 gaggcctcgc tggaagccgt gcactggaca tcgaccctgc agttccccga cttcgagcag
     1741 gactatgagt tcgtcgcgct gcgccatccc gacgagtacc ccttcaacga aggacgcctg
     1801 gtctcaaatc gcggcctcga cattgacgcc gcggaatatg aggaccgatt tgtcgagctg
     1861 cacgtcaagc attcgaacgc gctgcattcc gtgatccgtg ggcgcggctc atatctggtc
     1921 ggtccgttgg cgcgcttcaa cctgaatttc gacaagctgc ccgaagtggc ccagaaagct
     1981 gcgctcgagg cgggtctgaa accgcccgtc aaaaacctgt tccgcagcat cacggtccgg
     2041 gctgtggaac tcgtctttgc cttcgccgag gcgctgcgtg tgatccgcga gtacgaaccg
     2101 ccctctgcac cgcgcgcgat agtccctgac agggcgggag tgggccaggc gatcaccgag
     2161 gctccgcgcg gcatcctgta tcaccgctac gcggtcgacg aaaaaggact catcgtcact
     2221 gccaagatca ttccgccgac ttcgcagaac cagcgccgga tcgaggacga tctgcgcgag
     2281 tacgccgcgc aactggcggc gtggccgctc gaagaggcaa cctggaagtg cgagcaggcg
     2341 gtgcgctgct acgacccctg catctcctgt gccacacatt ttttgaaagt cgaaatcgac
     2401 cgggagtagg aatccgcgaa tcacgcaacc cggccgatca cgccgacggg atccacaaca
     2461 tgttgattat cggctgcggc aatcgccagc gcggggatga cggtgccgga atcatcgccg
     2521 tcgagcgcct gcgcgctctc ggcattcctg caaaaatctg caccggcgaa tcttccgaac
     2581 tgatagacgc ctggtctggc gcggatgacg ttgtcgtcat cgatgccgtg gtgactggtg
     2641 cgccaacggg cactgtgcac gtgtgggatg ggcagcatcc catgcggccc tccaggcctt
     2701 cggcatccac acacgggatc ggcgttgctg aagccatcga gctggcgcgt acactcggcc
     2761 gcttgccccc taagttgcga gtgctcggaa tcgaaggaaa aacatttaaa atgggaaaca
     2821 atatctctcc cgaagtcgaa cgcgctgtcg aacacgtggt agagcgaatc gcgagagaag
     2881 ttggcgctgc taaataggcg catgcgtctc cagaaacatg gatcgcatta cgccgtcccg
     2941 gcgttggtga aggcctcgag cgcgtcggag agcgtgggga aaatcaggaa gacctgttcg
     3001 accttggtaa tttcgaagac cttttgcacg cgctggttca cgcctgtaat tgcgatgcgg
     3061 cgtccggcct tggcg
//
LOCUS       DASPUL010000500         2993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_503462, whole genome shotgun sequence.
ACCESSION   DASPUL010000500 DASPUL010000000
VERSION     DASPUL010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503462"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /gene="xseA"
                     /locus_tag="VEJ00_11885"
     CDS             <1..1214
                     /gene="xseA"
                     /locus_tag="VEJ00_11885"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXX01906.1"
                     /translation="FTLKDGNAQIRVVMFRTSARLLRFRPVDGLQVIVRGRVTVYEDR
                     GELQISAEYMEPKGAGSLQLAFEQLRAKLEAEGLFAAERKRPIPSLPARIGIVTSTQA
                     AALRDILNVLARRHHSVNVLIYPAQVQGDAAAYEVAAGVRYFNQQSADQPNRVDVIII
                     ARGGGSAEDLASFNDEGLARTVAASQIPVISAIGHETDFTIIDFVADLRAPTPSAAAE
                     LVIRSRQEIEDQAAALHQRLGRAVRYRLLMGRQALTELAQHGAFARMMEAIHQRQQKL
                     DDLTHRLELAQRLLLEQAHRRWETVSAAVRHYDLRLMLAGMRRELETGTAALASAMRN
                     VLLQHRVRSDRLETALQSLSPLAVLERGYALVFDAQGKLLKDARRVKVGEEISARLNH
                     GEIRASVTRKG"
     gene            1305..2192
                     /gene="cdaA"
                     /locus_tag="VEJ00_11890"
     CDS             1305..2192
                     /gene="cdaA"
                     /locus_tag="VEJ00_11890"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HXX01907.1"
                     /translation="MTPVFTSWSQGAWAALDILLVAILIYQLLVMIRGTRAAPMLAGL
                     AAVALAFYLAPTQKLTTLNWAVSWVLPYVVFALIVVFQVEIRHMLADLGRRLSFLGGS
                     AAESDSYDDIVLAANLFSQHQTGALIVIERRIGLRTHIESGVPLDARLSYDLLATIFR
                     PSAPLHDGAVIVQKDRIAAAACFLPLSMNPLLSTQLGTRHRAGIGITEETDAIAVIVS
                     EETGAISLAVGGNIERDLTVEQLRERLGNELRRYMAPVTLPTPIAQNGEESDAPVDSP
                     LREASQHAAESRADSEPLP"
     gene            2189..2866
                     /locus_tag="VEJ00_11895"
     CDS             2189..2866
                     /locus_tag="VEJ00_11895"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HXX01908.1"
                     /translation="MIAFFRRYVLHNFGLKILSLLLAAGLWFLISRDEEPAEVALRAP
                     IVFQHVPPHLEISSEAIPEAQIRVRGPERVIRRLQASEVHAEIDLSNARPEEHTYDLT
                     SQQVRHPRDVTVVQVVPSQLHLAFDTRLTRDVDIHPRITGIFSEGEQIVKVLSDPARI
                     TITGPRHHVEITDAATTDPVDASGTMGSAVFTTNVYVADPLVQVVQPISVRVTVIVEK
                     VGNASAH"
BASE COUNT          539 a         1010 c          856 g          588 t
ORIGIN      
        1 atttcacgct gaaagacggc aatgcgcaga ttcgcgtcgt gatgttccgc acctcggcgc
       61 gcctgttacg ctttcgcccg gtcgacggat tgcaggtcat tgtgcgcggc cgtgtcaccg
      121 tctacgaaga ccgcggcgaa ctgcagattt cggccgagta catggagccc aagggcgccg
      181 gatcgcttca gcttgccttc gaacaactca gggccaagct cgaagccgaa ggtctcttcg
      241 ctgccgaacg caagaggccg atcccctcgc tgccggcgcg catcggcatc gtcacctcga
      301 cgcaagccgc agcgctgcgc gacattctga atgtgctcgc acgccgtcat cattcggtta
      361 acgtgctcat ctatcccgcg caggtgcaag gcgacgcggc ggcctacgaa gtggcggcgg
      421 gtgtgcgcta cttcaaccag caaagcgccg accagccgaa ccgtgttgat gtcatcatca
      481 tcgcgcgcgg cggaggctcg gccgaagatc tcgcttcatt caatgatgaa ggcctggccc
      541 gtaccgtggc cgcgtctcag attccggtga tctccgccat cggccacgag accgacttca
      601 cgatcatcga cttcgtagcc gacctgcgcg cccccacgcc atccgccgcc gcggaactgg
      661 tgatccgctc ccgccaggag attgaagatc aggctgccgc gctgcaccag cgcctcggcc
      721 gcgccgtgcg ctaccgtctg ctgatgggcc ggcaggcact caccgaactc gcccagcacg
      781 gcgccttcgc acgcatgatg gaagcgattc atcagcgcca gcagaagctc gacgatctca
      841 cacaccgtct ggaacttgcg caacgtcttc tgctcgagca ggcacaccgg cgctgggaaa
      901 cagtctcggc ggccgtgcgc cactacgact tgcgcctgat gctcgccggc atgcgccgcg
      961 agttggaaac cgggaccgca gcactggcct ccgcgatgcg gaacgttttg ttgcaacaca
     1021 gagtgcggag cgatcgcctc gaaaccgccc tgcagtcgct ctcccccctg gcagtgctcg
     1081 agcgcggata tgcgctcgtg ttcgacgccc aaggcaagct gttgaaggac gcacgccggg
     1141 tgaaggtcgg cgaggagatt tccgcccgcc tcaatcacgg ggagattcgc gcctctgtga
     1201 ccaggaaggg atgaggaatc ggcacggggc cgtcgttgaa gcgatgctcc gctgcattac
     1261 aatgagcttt ccgctctaaa atcagcatca ggagaaagac tcgagtgacg cctgtcttca
     1321 cgtcgtggtc gcaaggcgca tgggcggcgc tcgacattct tctggttgcc atcctcattt
     1381 accagctcct ggtgatgatc cgcgggactc gtgccgctcc gatgctggct gggctggccg
     1441 ccgtcgctct ggccttttat ctcgctccga cccaaaaact gaccaccttg aattgggccg
     1501 tcagctgggt tctgccctac gtcgtgttcg ccctgatcgt ggtattccag gtcgaaattc
     1561 gccacatgct ggccgatctt ggccggcgac tgagcttcct gggcggctcc gccgcagaaa
     1621 gcgattccta cgacgacatc gttctggccg ccaatctatt ttcccagcat cagaccggcg
     1681 ctctcatcgt gatcgagcgc agaatcggac tgcgcaccca catcgaaagc ggagtcccgc
     1741 tcgacgcccg cctttcctac gacctgctgg ccacgatctt ccgccccagt gctcctctgc
     1801 acgatggcgc cgtcatcgta cagaaagacc gcattgccgc cgccgcctgt tttctgccgc
     1861 tttccatgaa tccattgctc tcgacgcagc tgggcacgag gcatcgcgcc ggcatcggca
     1921 tcaccgaaga gactgatgcc atcgcggtca tcgtttccga ggagaccggc gctatcagtc
     1981 tcgccgtcgg cggcaacatc gagcgcgatt tgacggtgga acagttgagg gagcggctcg
     2041 gcaacgaact gcgccgttac atggcgcccg ttacccttcc gactccgatc gctcagaacg
     2101 gcgaagagtc ggatgcgccc gtcgactctc ccctgcgcga agcctcgcaa catgcagctg
     2161 agtcgcgcgc ggattcggag ccgctgccat gatcgctttc tttcggcgtt acgtcctgca
     2221 caacttcggg ttgaaaatcc tctctctgct gttggcggcg ggcttgtggt tcctgatctc
     2281 ccgcgacgag gaacctgccg aagtggcttt gcgcgctccg atcgtctttc agcacgttcc
     2341 gccgcatctc gaaatcagtt cggaggcgat tcccgaggcg cagatccgcg tgcgcggccc
     2401 cgagcgcgtc atacgccgac tccaggccag cgaggtgcac gccgagattg acctctccaa
     2461 tgccaggcct gaagaacaca cctatgacct cacttcgcag caggttcgtc atccccgcga
     2521 cgtgacggtg gtgcaggtcg tgcccagcca gctccacctt gctttcgaca cgcgcctcac
     2581 tcgcgacgtc gacattcacc cgcgcatcac cgggatcttc agcgagggcg agcagatcgt
     2641 taaggttctt tccgatcccg cacgcattac catcaccggg ccgcgtcacc acgtcgaaat
     2701 aacggatgcc gccactaccg atccggtcga cgcgagcggc accatgggca gcgccgtctt
     2761 caccacgaac gtctacgtcg ccgacccgct ggtgcaagtt gtgcaaccga tttcggtccg
     2821 cgtcaccgtg atcgtcgaga aggtgggcaa cgcgtcggcg cattgaaacg cttaacccgc
     2881 cacttagttt gccattccga cctgaccgca gcgaagggag aaatctgcct tttctctctc
     2941 gggcacgttc tcatatatca tctctgttgc tcttcttcat gcaccttccg gaa
//
LOCUS       DASPUL010000501         4254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1869455, whole genome shotgun sequence.
ACCESSION   DASPUL010000501 DASPUL010000000
VERSION     DASPUL010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869455"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VEJ00_11900"
     CDS             <1..784
                     /locus_tag="VEJ00_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXX01909.1"
                     /translation="LHALSARTARPLVAVNTGAIPDGVFESELFGHVKGAFTDARTDR
                     IGRFELADGGTIFLDEIGNVPLRQQAKLLRVLESGEIERVGSSRARRINVRVLSATNT
                     DLPTACAAGQFREDLLFRLNTVEIHVPALRERREDIPVLAGHFLARYSARYRRPVRGF
                     DPGAIQALKQYSWPGNVRELEHTIERAVLMCRSEEIEPADLSLSSQRAQTQNLEELSL
                     EAVEALLVRKALQRFQGNVSQAAEALGLSRGALYRRMEKYGV"
     gene            918..2186
                     /locus_tag="VEJ00_11905"
     CDS             918..2186
                     /locus_tag="VEJ00_11905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXX01910.1"
                     /translation="MGSRLALVAVLLFVWWILAMALQEQTIRPLQTLANVIGSLRDED
                     YSFRARNAVPEDALGELSLEVNLLADMLSGHRLRSIEATALLQRVVDEIDAPLFAFDP
                     ASALRLANPAGEHLLHQAKARMLGRSATELGLQQCLDADNESLVELNLADSRPRWLLR
                     RSSFRQNGVPHTLVVLSDVSRALRDEERRAWQRLIRVLGHELSNSLAPIKSIAGSLGA
                     RVSAAPMDSDVRSDLQRGLEIIEARSASLHRFLEAYRRLAQMPAPVLREVPLGPLVAR
                     TAGLETRVKVYVQPGPSVVFQADPDQLEQMFINLVRNAADAVLETPNGSSRSGSDPVI
                     VHWDADEKDVTLTIDDNGPGLSNPANVFTPFYTTKPNGSGVGLVLSRQIAEAHGGRIE
                     IGNRINSRGCSVKVVLPRSPSRHAPMSRQL"
     gene            2262..3932
                     /locus_tag="VEJ00_11910"
     CDS             2262..3932
                     /locus_tag="VEJ00_11910"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXX01911.1"
                     /translation="MSYCKTVVAVLVVFLLSLASVTSLQAVDKKFHDAPDSAKALQNP
                     YAQPSAAVLNNGKTLYARNCLSCHGRAGKGTGNVPSLVNGKLDGVPAGEVFWFISKGD
                     KDNGMPAWDFLPEQKRWQIVTYVEALASGKAEAPAAAAAPAEGSMAKIKDASPQAPFT
                     DFRYESPGTTRKITVNDLPEPYVSKSADNGPDVVARPQDAWPMALPGFKVELYAGGLD
                     NPRLLRTAPNGDIFLAESDPGRIRVFRGMTGDGKAEQSAIFASGLKQPYGIAFYPPGP
                     DPQWVYIGNTNELIRFPYHNGDMKASGPSQHIADLPSTSFGHWTRAVEFSADGKKLFV
                     AVGSGSNVDDPDTHPSEKDRADILVCDPANCVLSVYASGIRNAGGGIAVNPQTGELWC
                     SVNERDALGDNLVPDYITHVQEGGFYGWPWWYIGSHQDPRHKGKHPELKDKAIVPDVL
                     LQPHNASLELTFYQADKFPAEYKGDIFAAEHGSWNKATRAGYEVIRVPLHQTGHATGE
                     YEDFLTGFVTPGGQVWGRPVGITVAADGSLLVSDDGSNSIWRVSYLGK"
     gene            complement(4021..>4254)
                     /locus_tag="VEJ00_11915"
     CDS             complement(4021..>4254)
                     /locus_tag="VEJ00_11915"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HXX01912.1"
                     /translation="PGTAKGFVFISLEDETGVANAIITPDLFHQNRLLLASERFLAIE
                     GILQNQDNVISVRAERVQPLFVTKAETVSHDFH"
BASE COUNT          820 a         1239 c         1342 g          853 t
ORIGIN      
        1 tctgcacgcg ctttctgcgc gaactgcgcg cccgctggta gccgtgaata caggagccat
       61 tcccgacggg gtcttcgaaa gcgagttgtt tggacacgtg aaaggtgcgt tcaccgatgc
      121 gcgcactgat cgcatcggcc gcttcgaact ggcggacgga ggcacgattt ttctcgacga
      181 gatcggcaat gtccctctgc gccagcaggc caagctgctg cgtgttttag aatctggcga
      241 aatcgagcgc gtgggctcgt cgcgcgcccg gaggatcaac gtgcgggtgc tctcggccac
      301 caacacggac ctgccgacgg cttgtgccgc cggacaattc cgcgaggatc tgctcttccg
      361 gctgaacaca gtggagatcc atgttccggc tctgcgcgag cggcgggaag acattcctgt
      421 actggcaggg cattttctgg cgcgctattc ggcccgctac cggcgcccgg tccgaggatt
      481 cgatcccggg gccatccagg ccttgaaaca gtactcctgg cccgggaacg tgcgcgaact
      541 ggagcacacc attgaacggg cggtcctgat gtgccgaagc gaggagattg aaccggctga
      601 tctctcactc agttcccagc gcgcgcaaac gcagaacctc gaggagctga gcctggaggc
      661 tgtggaggca ttgctggtcc gcaaggctct gcagcgcttt caggggaacg tcagccaggc
      721 ggctgaggcc ctggggttga gtcgcggggc actttatcga agaatggaga agtatggggt
      781 ctagctccac cccgacttcc cggccaaaaa gacgaacccg cctcctctac gaacgccgaa
      841 tcagtctcta ctccttcttc gtggcgctgc ccggcctgct tgtggctgga gcactgatat
      901 ggcagcagcc atggagcatg ggctcacgtt tggcactcgt ggccgttctg ctcttcgtct
      961 ggtggatttt ggccatggcg ctgcaggagc aaaccattcg gccgctgcag acgctggcca
     1021 atgtgatcgg gtcgctgcgc gatgaggatt actccttccg cgcgcgcaac gcggtgcccg
     1081 aggatgcgct aggcgagcta tcgcttgaag tcaatctgct ggccgacatg ctttccggtc
     1141 acagactgcg aagcattgag gctaccgcgc tgttgcagcg cgtggtcgat gaaattgatg
     1201 cacccttgtt tgcttttgat cctgcctcgg ctctacgcct ggcaaatcct gcaggggaac
     1261 atctgctgca ccaggcaaag gcgcgaatgc tgggacgtag cgctaccgag ctcggcctgc
     1321 aacagtgcct ggatgccgac aacgagagcc tggtggagct gaatctcgct gactcgcggc
     1381 cgcggtggct gctgcggcgc agctcgtttc gccagaacgg agtgccccac acgctggtgg
     1441 tgctttcgga cgtgagccgg gcgctgcgcg acgaagaacg gcgggcatgg cagcgcctga
     1501 tccgcgttct cggtcacgaa ctgagcaatt ctctggctcc gatcaagtcg atcgccggga
     1561 gtcttggcgc ccgcgtatcc gcagcaccga tggactcaga cgtgcgcagc gatctgcagc
     1621 gcgggctgga gatcattgag gcgcgttccg cgtcgcttca ccgctttctt gaagcctatc
     1681 gcaggctggc gcagatgcca gctccggttt tacgagaggt gccgttgggc ccgctggtcg
     1741 cccgcacagc aggattggaa acgcgagtca aggtatacgt acagcccggg ccgagcgttg
     1801 tattccaggc tgatccggat caactcgaac agatgttcat caatctcgtg cgaaacgcgg
     1861 ccgacgcggt gctggagacg cccaacggat cgtcccggtc cggctctgac ccggtgattg
     1921 tgcattggga cgccgacgag aaggacgtaa ctttgacgat cgatgacaat ggccccggcc
     1981 tctccaatcc ggccaacgta ttcactccct tctataccac caagcccaac ggcagcggcg
     2041 ttggcctggt tctttcgcgc cagatcgccg aggcgcacgg cggcagaatc gagatcggta
     2101 accggatcaa cagccggggc tgctcggtga aggtcgtatt gcctcggtca ccatcgcgcc
     2161 acgctccgat gtcccgtcag ctttgaatga gtcggcggtg aaattcgaca tccccggcgt
     2221 ggcccgctta atctaatagg aaatctcccg agaggatgcc catgagttac tgcaagactg
     2281 tggtcgccgt tctcgtcgtt tttttgctct ctcttgcttc cgtgacatct ttgcaggcgg
     2341 tggacaagaa attccacgat gcgccggact cggccaaggc tcttcaaaac ccgtacgcgc
     2401 agccgagcgc ggcggtcttg aacaatggca agaccctcta tgctcgcaat tgcctttcct
     2461 gtcacggaag ggctggcaaa ggcacaggaa atgtcccttc gctggtgaac gggaagctgg
     2521 atggggtgcc ggcgggcgag gtgttctggt tcatttccaa aggagacaag gacaacggaa
     2581 tgccggcgtg ggattttctc cccgaacaga agcgctggca gatcgtgacc tatgtggagg
     2641 cgctggcgtc gggcaaggca gaggctcctg ccgccgccgc tgcgcccgcc gaaggcagta
     2701 tggcgaagat caaggatgca tctccgcaag cgccctttac ggatttccgc tatgagtcgc
     2761 cggggacgac tcgcaagatt acggtgaacg atctgcccga gccctacgtg agcaagtcgg
     2821 cggataacgg gccggacgtg gtggcccgtc cgcaggacgc ctggcccatg gcgctacctg
     2881 ggttcaaagt cgaactgtat gccggcggac tggacaatcc gcgcttgctg cgcaccgctc
     2941 cgaatggaga catttttctg gcggaaagcg atccgggccg catccgcgtc ttccgtggaa
     3001 tgaccggcga cggcaaggcc gagcagtcgg cgatttttgc cagcggtctg aaacagccct
     3061 acggaatcgc gttctatcct ccgggaccgg atccgcaatg ggtttacatc gggaacacca
     3121 acgaactgat tcgctttccc taccacaatg gcgacatgaa ggccagcggt ccgtcgcagc
     3181 acatcgcgga cttgccctcc acttctttcg gccattggac gcgtgcggtc gaattctctg
     3241 ccgacggcaa gaaattgttt gtcgcggtcg gttctgggtc caacgtcgac gatccagaca
     3301 cgcatccctc cgagaaagac cgtgccgaca ttctggtatg cgatcccgcc aactgcgtgc
     3361 tcagcgtgta tgcctccggc attcgcaacg cgggcggagg aatcgcggtc aacccgcaga
     3421 ccggagaact gtggtgctcg gtcaacgagc gtgacgctct tggcgacaat ctcgttcctg
     3481 actacatcac gcacgtacag gagggtggat tttacggctg gccgtggtgg tatatcgggt
     3541 cgcaccagga tccgcggcac aagggcaaac accccgaact gaaagacaag gcgatcgtac
     3601 ccgatgtgct gctgcagcca cacaatgctt cgctggaatt gacgttttat caggcggaca
     3661 agtttcctgc cgagtacaag ggtgatattt tcgctgccga gcacggatcg tggaacaagg
     3721 caacccgtgc gggatatgaa gtgatccgcg taccgctgca tcaaacgggc catgctaccg
     3781 gtgagtatga ggatttcctg acgggattcg taacgccggg cggccaggtg tgggggcgtc
     3841 cggtggggat cacggtggcg gcggatggct cgctgctggt gagcgacgac ggctcaaact
     3901 cgatctggag ggtgagctac ctggggaagt aagcggggca cactttagct gattcggcag
     3961 tttgacaatc aagaaccgat aagcagttct ccgtgactcg gtgtctccgt ggtgaagaac
     4021 tcagtggaaa tcgtgggaga cggtttcggc tttggtgacg aatagcggtt gcacgcgttc
     4081 ggcccgcacg gagatgacat tgtcctgatt ctgcagaatt ccctcgatcg cgaggaatcg
     4141 ctcgctggcc agcagcaggc gattctggtg aaaaagatcg ggcgtgatga tggcgttggc
     4201 cacgccggtt tcatcttcca ggctgataaa gacaaaacct tttgccgtgc ccgg
//
LOCUS       DASPUL010000502         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1462588, whole genome shotgun sequence.
ACCESSION   DASPUL010000502 DASPUL010000000
VERSION     DASPUL010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462588"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..>2298
                     /locus_tag="VEJ00_11920"
     CDS             302..>2298
                     /locus_tag="VEJ00_11920"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HXX01913.1"
                     /translation="MLLSKMTLDEKIELIGGINDFYTRPIPRLGIPQLRMSDGPMGVH
                     DYGLTTAYPAGIALAASWDTDLARRFGISMGHDARARGVHFILAPGMNIYRAPMCGRN
                     FEYFGEDPFLASRMAVSVINGIQSQRVIATAKHFAGNNSEFARMTLSSNIDERTLREI
                     YLPAFEASVKEAKVGAVMDAYNLVNGVHMTQNNHLNNEILKKEWGFDGILMSDWEATH
                     DGVAAALNGLDLEMPSPAFMNRETLLPALKAGKIPISTLDDKVRRILRKAIEFGFLDQ
                     PQTDETIPLYSQEGRQVALEVARSSIVLLKNSRNVLPLDDSKVRTIAVIGPDAYPAVP
                     GGGGSSETKPFRAVSYLEGISDRLGSKAKVLYTTDVPPLDEVFENSDFVVSPGGESGL
                     KGEYFANEELQGSPALVRIDKHIHFDWGEGSFAPNEPVDHFSARWTGYYVPKKSGDYT
                     FYTSADDGVRLYIDDDSGIDDWQRHAQTVDSYARHLKAGHAYKIRLEYFENVGSAIVG
                     FGVTSVESFIGRATKALAAKADAVVICVGFDPKTESEGSDRTFQLPGGQDELIRQIAS
                     VNPNTIVVITAGGNVDMTQWVDRVPAILHAWYPGQEGGRALAQILLGDYSPSGKLPAS
                     FERRWEDNPTFHNYYPESGKSSVQYAEGVFLGYRYYDRSAT"
BASE COUNT          512 a          613 c          682 g          491 t
ORIGIN      
        1 caatcttgcg gtggggggag actgggcggg cagtcctgat gcgaccacgg tttttccgcg
       61 ggacatgcct gtggattacg tgcgggtgta cgagcgcgta agacgctagc ggcgagatac
      121 cgaagttctc ccgtagtctt cgaaacgttt gatcgacggg atccagattg tcgtttacgg
      181 agctcaagaa tcggagatga aagtcagtta ccgaaatcta cgggcggtcg tccttctttt
      241 cctggttgcg cgatttgcag tggctcaggt cgcgtcttca gaggtggaaa ggcgcgtcga
      301 tttgctactg agcaaaatga ctctggacga gaaaattgag ctgatcggcg ggatcaacga
      361 tttctacacc cgcccgatcc cgcgacttgg gattccgcaa ctgcgaatgt cggatggccc
      421 tatgggagtc catgactatg ggctcacgac agcgtacccc gccggcatcg cgttggcggc
      481 ttcgtgggac actgacctgg cacggcgctt cggtatctct atgggtcacg atgcgcgcgc
      541 acgcggcgtg catttcattc ttgcccccgg catgaatatt tatcgcgcgc cgatgtgcgg
      601 ccgcaacttc gaatatttcg gagaagatcc ctttttggct tcgcgaatgg ctgtttccgt
      661 catcaacgga atccaaagtc aacgagtgat cgcaacggcg aaacactttg ccgggaacaa
      721 ctctgagttt gcgcgcatga cgctcagttc gaacatcgac gagcggaccc tgcgcgaaat
      781 ctacctgccg gcattcgaag cttcggtcaa agaggccaag gtaggggccg tgatggatgc
      841 ttataacctg gtgaatggcg tccacatgac tcaaaacaat catctgaaca acgagattct
      901 caaaaaggaa tggggttttg acggaatcct catgtccgat tgggaggcaa cgcacgacgg
      961 ggtggcggct gcgctgaacg gcctcgatct ggagatgccc tcgccggcgt tcatgaaccg
     1021 tgagacgctg ctcccggctc tcaaagcagg gaagattcca atctcgacct tggatgacaa
     1081 ggtgcgaaga attctgcgga aggcaatcga atttggcttt cttgatcaac ctcaaacgga
     1141 cgaaaccatt cctttgtaca gtcaggaggg ccgacaggtt gccttggagg tcgcccgtag
     1201 cagcatcgtc ttgttgaaga actcgaggaa tgtgcttccc ctggacgaca gcaaagtgcg
     1261 aaccatagcg gtcatcggtc cagatgctta ccctgccgta cctggcggag gcggcagctc
     1321 tgaaaccaaa ccgtttcgcg ccgtcagcta cctggaaggc atcagcgatc gtctaggaag
     1381 caaggcgaag gtgctataca cgacggacgt tccaccgctc gatgaagtct tcgagaattc
     1441 agattttgtc gtgtcaccgg gtggcgagag cggactgaag ggcgagtact tcgccaatga
     1501 agaactgcag ggcagtcctg ctctcgtgcg cattgacaag catattcact ttgattgggg
     1561 agaaggtagc ttcgctccga acgaaccggt ggatcatttt tcggctcggt ggaccggcta
     1621 ctacgtaccg aagaaatcgg gggactacac gttctatacg tccgccgacg atggagtccg
     1681 cctgtacatc gacgacgata gcgggatcga tgattggcag cgccacgcac aaaccgtcga
     1741 tagttacgcg agacatctca aggcaggtca tgcctacaag atccgccttg aatatttcga
     1801 aaacgtaggc agcgccatcg tgggttttgg cgtgacgtcc gtggagtcgt ttatcggccg
     1861 cgcaaccaaa gcgctcgccg ccaaggcaga cgcggttgtg atttgcgtgg ggttcgaccc
     1921 gaagacagag agtgaaggct cggaccgaac cttccagctt cctggcggac aagacgaact
     1981 gatccgccag attgcttcgg tcaatcccaa tacgattgtc gtgataaccg ccggcggcaa
     2041 tgttgacatg acccagtggg tggatcgtgt gccagcgatt ctgcacgcct ggtatcccgg
     2101 acaggaaggc gggagggctc ttgcgcaaat cctgctcggc gactacagcc cctcgggcaa
     2161 acttcccgca tctttcgagc ggcgctggga ggacaatccc acctttcaca actactatcc
     2221 cgagagcggc aaatcgagcg tccagtatgc ggaaggcgtt ttcctgggtt atcggtacta
     2281 cgatcgctcg gcgacgaa
//
LOCUS       DASPUL010000503         5372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1190713, whole genome shotgun sequence.
ACCESSION   DASPUL010000503 DASPUL010000000
VERSION     DASPUL010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190713"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..2463
                     /locus_tag="VEJ00_11925"
     CDS             130..2463
                     /locus_tag="VEJ00_11925"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HXX01914.1"
                     /translation="MRILSARLIVALIVGVTLVSLCSSGYEVLVEKQNLRRDLQKRSE
                     TLGESLAGGVEHDLEKNEVHTLQRTVQRFANRENLAGLAVYDPQGHVIAVTAELAPSM
                     SEAPGVVVQSLKQNQEADVFQRLANVPVHICALPLHSQDKLLGVLALVHDAGYIREQS
                     LHIWRETFLSALAHVVLIALIVLLIIRWSVQGPIARTAHWMRALRTGRLPFSRIAAPD
                     LELFRPLAREVATFAESLNTARTAAEREARLRDEAQSQWTAERLAAHVRAKLGSGRLF
                     VVSNREPYVHMRQGKNVEVSVPPSGLVTALEPVLVACDGTWIAHGNGNADRETVDRYN
                     KLRVPPDDPRYTLRRVWLTKEEEQGYYYGFANEGLWPLCHIAHTRPIFRADDWKQYET
                     VNRKFADALLDEMRDTENPVVLVQDYHFTLLPRLIKQKRPDARVAIFWHIPWPNPEAF
                     GICPWQRELVDGLLGADLIGFHIQSHCNNFLQTADRILESRIDREHFSVQRRGHVTMV
                     RPFPISVDASDEEPGASEETAYETRSSLLKGLDVEAIFLGVGVDRLDYTKGILERFLA
                     VERFLEKYPQYQGQFTFVQIGAPSRSHLKRYHDLQAEIEEEAGRINWRFQKDKWKPIV
                     LLTRQHSHKEIQPYYKAADLCLVTSLHDGMNLVAKEFVAARSDECGVLILSIFTGAAR
                     ELRDALQVNPYDIDQTAEAIRAALEMEPADKQARMQRMRRAIREHNVYQWAGNLIAEL
                     CELRLDLHEDPRRNNPQIGARGTPMKEIANASHAPGQ"
     gene            2438..3154
                     /locus_tag="VEJ00_11930"
     CDS             2438..3154
                     /locus_tag="VEJ00_11930"
                     /inference="COORDINATES: protein motif:HMM:NF039842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HXX01915.1"
                     /translation="MPATRRGNDGTGSVTSESRRAERKPTRPFYFNTSEHLLRISRQR
                     AATLGELHQTLRECDEASIFQHTFRTLQEHHFIRQGFSNDFAHWALSVCHEPVLAEQL
                     ASVDVREFTSIQGLRDRMVDIVGEFIRTHPHAAARPAHEPFYFCAADLVVLPTPFVSD
                     TLGAFVDALRRVSVHSIHHHFIEARLRLHEMSNDFSRWLEEEAGLAEAGQAVERIDIY
                     TNTMEGVRQQIASIVEKAAN"
     gene            3167..4576
                     /locus_tag="VEJ00_11935"
     CDS             3167..4576
                     /locus_tag="VEJ00_11935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXX01916.1"
                     /translation="MDATKTSAPPKKLNPSATVADGRTEAESAAGSSLAAAPAVEPAS
                     SPFRTTTRLPSSLLEPPAPPPPPHLDDYESITGSAQIDALRFLANELKGKTIKMVNST
                     SVGGGVAEMLNRLVPLLSELEVPAHWDVITGGNDFFEVTKAFHNALHGSPYELTKNAQ
                     DIFLMYNEQNMQRMKFEEGMVVIHDPQPAALIRAKDKTDAKWVWRCHIDLSNPHAQLW
                     EFLRPFIESYNAAIFSSQSFTRQLPIPQYLFYPCIDPLSEKNKDLDDSFVQKVCDDFG
                     IDRRRPIVTQVSRFDRLKDPVGVIHAYKLAKKYVDCQLVLAGGGAKDDPEGSVVLQEV
                     KEAAGNDPDIIILDLPPWCALEINAIQRASTLIVQKSIREGFGLTVTEALWKKKPTIA
                     GAVGGIPNQIIHRLTGSLVHSVEGCAYQIRYLLTHPEFARRIGMNGHEHVKENFLITT
                     NVKRWLLLYRILAQKKAAS"
     gene            complement(4592..>5372)
                     /locus_tag="VEJ00_11940"
     CDS             complement(4592..>5372)
                     /locus_tag="VEJ00_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01917.1"
                     /translation="LIRAAEKIAPREISALRPRLKKLFGTAPEQNVVNERSIRLYLAL
                     RKLVAREKFDFYTIQSFPGLGDDYSATCFAQSMMLEDGHGTSTLGDFNTALTVLLLTK
                     LSKERVYYGDLQHIDKKKNEIKIIGDGACPPSLASKLGPAGFSEHGIPTEGEAGGLSV
                     RLICKVGEGVLARLGRVNGEFQMVITRASIFEPPKDQVKKRLNECGIPFWPHGFVTAH
                     CDIDKMLQSWTNEYACLGYGADLYPALIDFCELTGIKAVLP"
BASE COUNT         1088 a         1662 c         1606 g         1016 t
ORIGIN      
        1 agatcaactc cagaaagact tcgtcttcgg caacagctca ggacccagtt gcgagggagc
       61 ttcgcgccac ttcccgactc gcggcgggcg gctgtctggt catagccgcc tccgagcaag
      121 gagtgtcgca tgcgaatcct cagcgcccgg cttattgtcg ctctgatcgt cggcgtcacc
      181 ctggtgtcgc tatgctcctc cgggtacgaa gttctggtgg agaagcagaa ccttcgccgt
      241 gatctgcaga aacgctccga aacgctcggg gagagcctcg ccggaggcgt cgagcacgac
      301 ctcgaaaaga acgaggtcca cactctgcag cggacggtgc agcgtttcgc caatcgcgag
      361 aaccttgccg ggctggctgt ttatgatccg cagggccatg tcatcgccgt caccgccgag
      421 ctggctccga gcatgagcga ggcgcccggc gtcgtcgttc agtcgctgaa gcagaatcag
      481 gaagcagatg tttttcaaag actggccaac gtcccggtcc atatctgcgc cctgccactg
      541 catagtcaag acaagctgct cggggtgttg gccctggttc acgacgcggg atacatccgg
      601 gaacagagcc ttcatatctg gcgcgagacg tttctgagcg cgctggcgca cgtggtcctg
      661 attgcgctca tcgtgctgct gatcattcgc tggagcgtcc agggaccaat cgcccgcacg
      721 gcgcactgga tgcgggcttt gcgcaccgga cgcttgcctt tctcgcgcat cgccgcgccc
      781 gaccttgagc tgtttcgccc gctggcgcgc gaggtcgcca ctttcgccga gagtctgaat
      841 acggcccgca cggccgccga gcgcgaggcg cgactgcgcg acgaggccca gtcgcaatgg
      901 acggcggagc gcctggcggc gcacgtccgc gccaaactcg gctccgggcg gctgtttgtc
      961 gtttcgaacc gcgagcccta tgtccacatg cggcaaggca agaatgtgga ggtcagcgtg
     1021 cctcccagcg gtctggtgac tgcgctcgag cccgtgctcg tcgcctgcga tggaacctgg
     1081 atcgctcatg ggaatggcaa cgccgaccgc gagaccgtgg atcggtataa caagctgcgc
     1141 gtgcctccgg atgatccgcg ctataccctg cgccgcgtct ggctgacgaa agaagaagag
     1201 cagggctatt actatggatt cgccaatgag ggactctggc cgctctgcca catcgcgcac
     1261 acgcgtccaa tctttcgcgc cgacgactgg aagcagtatg agaccgtgaa tcgcaaattc
     1321 gccgatgccc ttctggacga aatgcgggac accgagaatc cggtcgtgct ggtgcaggac
     1381 taccacttca cgctgctgcc ccggctgatc aagcaaaaac gcccggacgc ccgtgtggcc
     1441 atcttctggc atattccgtg gccgaatccg gaggcgttcg gcatctgccc gtggcagcgc
     1501 gaactggtgg acggcttgct gggcgctgac ctcatcggat tccacattca gtcccattgc
     1561 aacaactttt tgcagacggc ggatcgcatt ttggaatcgc gcatcgaccg ggagcacttc
     1621 tcggtgcagc gccgcggaca cgtcaccatg gtgcggccgt tcccgatcag cgtagatgct
     1681 tccgatgagg agcccggcgc ttccgaagaa acggcctatg aaacgcgctc cagcctgctc
     1741 aagggcctcg acgtggaagc gatttttctg ggagtcggcg tggaccggct cgactacacc
     1801 aagggcattc tggagcggtt cctggcggtc gagcgattcc tggagaaata tccgcagtac
     1861 caaggccagt tcacgtttgt gcagattgga gcgcccagcc gcagccactt gaagcgctat
     1921 cacgacctgc aggcggagat cgaggaagag gcagggcgca tcaactggcg cttccagaaa
     1981 gacaagtgga agccgattgt cttgctgacg cggcagcaca gccacaagga gatccagcct
     2041 tactacaagg ccgcagatct atgtctggtg acttcgctgc acgacggcat gaatctggtt
     2101 gcgaaagaat tcgtggccgc gcgcagcgat gaatgcggtg tgttgatcct gagcatcttt
     2161 accggcgcgg cgcgggagct gcgcgacgcg ctgcaggtaa acccgtacga catcgaccag
     2221 acagcggagg ccatccgggc cgcgctggag atggaaccgg ccgacaaaca ggcccgcatg
     2281 caacgaatgc gccgcgcgat tcgcgagcac aatgtctatc agtgggccgg caacctgatt
     2341 gctgagcttt gcgaattgcg cctggacttg catgaggacc cgcggcgaaa caatccgcag
     2401 attggagccc gcggtacccc catgaaggag attgccaatg ccagccacgc gccggggcaa
     2461 tgacggaacg ggaagcgtaa ccagcgaaag tcgacgggcg gaacgcaagc ctacgcggcc
     2521 cttctacttc aacacgtcgg aacacctgtt gcgcatcagc cggcagcggg cggcgacgct
     2581 cggagagctc caccagaccc tccgcgagtg cgacgaggcg tccatttttc agcacacctt
     2641 ccgcacgctg caggagcatc acttcattcg ccagggcttt tccaacgatt tcgctcactg
     2701 ggcgctttcc gtctgccacg agccggtgct ggcggagcag ctggccagcg tcgatgtgcg
     2761 cgagtttact tccattcagg gtcttcgcga ccgcatggtg gatattgtcg gagagtttat
     2821 ccgcacccat ccgcatgcag cggcgcggcc agcgcacgag ccgttttatt tctgcgccgc
     2881 cgacctggtc gtgctgccga ctccatttgt gtccgacaca ctgggcgctt tcgttgacgc
     2941 cttgcggcgg gtgagcgtgc actccatcca ccaccatttc attgaggcgc ggctgcgttt
     3001 gcatgaaatg tccaatgatt tctcgcgctg gctggaagag gaagcagggc tggcggaagc
     3061 gggacaggca gttgagcgaa tcgatatcta caccaacacg atggaaggag tgcggcaaca
     3121 gatcgcgagc atcgtcgaga aggccgcgaa ctagcaaggc aacgacatgg acgcaaccaa
     3181 aaccagcgca ccaccaaaga agctcaaccc gtcggcaacc gtcgcggacg gcaggacaga
     3241 ggcagaatct gccgccgggt cgtctctcgc cgcggcgcct gcggtcgaac ccgcaagctc
     3301 accctttcgc acgaccaccc ggctgcccag cagtcttctg gagccgccgg cgcccccgcc
     3361 gcctccgcac ctggatgact acgagagcat caccggctcg gcccagattg acgccctgcg
     3421 ctttctggcg aacgagttga agggcaagac catcaagatg gtcaactcca cgtcggtcgg
     3481 aggcggggtg gcagaaatgc tgaaccggct cgtgcctctg ctctccgaac tggaagttcc
     3541 cgcccactgg gatgtcatca ccggaggcaa cgattttttc gaggtgacca aggcttttca
     3601 caatgccctg cacggaagcc cctacgagct gacgaaaaat gcccaggaca ttttcctgat
     3661 gtacaacgag cagaacatgc agcggatgaa attcgaagag ggcatggtcg tgattcacga
     3721 cccgcagccc gccgcgctca ttcgcgccaa ggacaagact gacgcgaagt gggtctggcg
     3781 ctgccacatc gatctatcca acccgcacgc ccagctctgg gagttccttc gtccgtttat
     3841 cgaatcgtat aatgcggcga ttttctcgtc gcagtcgttc acgcgccagc tgccgatccc
     3901 gcaatatctt ttctatccct gcatcgatcc actctccgaa aagaacaagg atctggacga
     3961 ttcctttgtg caaaaagtgt gtgacgactt cggaatcgac cgcaggcgcc caatcgtgac
     4021 ccaggtgtcg cgcttcgacc gcctcaagga tcccgtgggc gtcatccatg cctataagct
     4081 cgccaagaaa tatgtggatt gccagctcgt gctggcgggt gggggcgcga aggacgatcc
     4141 cgagggttca gtcgtgctgc aggaagtgaa agaggcggcc ggcaacgatc ccgacatcat
     4201 cattctcgac ttgccgccgt ggtgcgcgct cgagattaat gccatccagc gcgcctccac
     4261 gctaatcgtt cagaagtcga tccgggaagg attcgggttg actgtgaccg aagctctgtg
     4321 gaagaagaag ccgacgatcg cgggcgcggt gggagggatt cccaaccaga tcatccatcg
     4381 gctgaccggt tccctggtgc actcggttga gggctgcgcc tatcagatcc gatatcttct
     4441 gacccatccc gaattcgcgc gcagaatcgg gatgaatggg catgaacacg tcaaagagaa
     4501 tttcctgatc accaccaacg tgaagcgctg gctgctgctc taccgcattc ttgcccagaa
     4561 gaaagccgca tcatgactcg cccaccaggc ctcagggtaa aactgctttg atccccgtga
     4621 gctcgcagaa atcaatcagc gcgggataca agtcggcacc atagccaaga caggcgtact
     4681 cattcgtcca gctctggagc attttgtcga tgtcgcagtg cgccgtgaca aacccatgcg
     4741 gccagaaggg aatgccgcat tcgttgagcc gcttcttgac ctgatctttc ggcggctcaa
     4801 agatgctggc gcgcgtgatc accatctgga attcgccgtt cacgcggccc agccgggcca
     4861 gcacgccctc gcccaccttg cagatcagcc gcacggacag tcctcccgcc tcgccttccg
     4921 tcggaatacc gtgctccgaa aacccggccg gccccagctt ggaggctaga gaagggggac
     4981 aggccccgtc gccgatgatc ttgatctcgt tcttcttctt gtctatgtgt tgcagatcgc
     5041 catagtagac gcgctccttg ctcagcttgg tcagcagcag aacagtcagc gcggtgttga
     5101 agtcgcccag ggtggaagtg ccgtgaccat cctcgagcat catgctttgc gcgaagcagg
     5161 tcgctgaata gtcgtcgccc aggccgggga aagactgaat cgtgtagaag tcaaacttct
     5221 cccttgcgac cagcttcctg agagcgagat acagtcgaat cgatcgctca ttcaccacgt
     5281 tctgctcggg cgcggtgcca aacagtttct tcagccgcgg ccgcagggcg gagatctctc
     5341 gtggcgcgat tttctccgcc gcccgaataa gt
//
LOCUS       DASPUL010000504         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1594819, whole genome shotgun sequence.
ACCESSION   DASPUL010000504 DASPUL010000000
VERSION     DASPUL010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594819"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VEJ00_11945"
     CDS             complement(<1..204)
                     /locus_tag="VEJ00_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01918.1"
                     /translation="MLTLPSNNKPKLTVLRARHLSEISWLVHGFSTRTGGFSRAYGSG
                     DLNLGFTKDDSRVSVERNREAFLR"
     gene            236..643
                     /locus_tag="VEJ00_11950"
     CDS             236..643
                     /locus_tag="VEJ00_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01919.1"
                     /translation="MFHFSVCGLRRASAIVLAMFENFTATDRWTKKQVHCVYQAMIVA
                     IATRHADAVDIKFLVDGRKVWVALPHPAWVEYKKRTRKLITDSLAQEIAGHYLKWALE
                     SGEGVGREMYSLTVEETLAHLDAVVAEMQAQPA"
     gene            complement(683..1369)
                     /locus_tag="VEJ00_11955"
     CDS             complement(683..1369)
                     /locus_tag="VEJ00_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01920.1"
                     /translation="MVTAVITPAPCGAMRPDMYKALTAVLLSLVVGLSACKRGESSPA
                     LADDLTAAEYNVLSAYTNGKFSGKPASNGVSKLVFFNTTGCNDRHLLSDDNGQLLPWK
                     RTAEFLRGKYPALQQATLDSFLRANLQQALVHSSFRLPVNYQLIDAAKETSIFRKGGG
                     SWDAFYQQYPGSSGIVTLSRVGFGVDGTQALFYFEKVCGGLCGGGYYVAMEKRDSGWV
                     IGAEINIWVS"
     gene            complement(1379..2995)
                     /locus_tag="VEJ00_11960"
                     /pseudo
     CDS             complement(1379..2995)
                     /locus_tag="VEJ00_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
BASE COUNT          593 a          892 c          887 g          630 t
ORIGIN      
        1 gcgcagaaac gcttcccggt tgcgttcgac gctaacccgc gaatcatcct tggtgaagcc
       61 gaggttgagg tcgccactgc cgtaggcacg cgagaatccc cccgtgcgag tcgagaaacc
      121 atgcaccagc caggagattt cactcagatg gcgtgcgcgg agtacggtga gtttgggctt
      181 gttgttcgag ggcagggtca gcacctgttc atcttaacgg agcttgcaga acctcgtgtt
      241 ccacttttcg gtttgcgggc tgcgccgggc gtccgctata gttctagcca tgtttgaaaa
      301 cttcacggcc acggatcgct ggaccaaaaa gcaggtccac tgcgtctatc aggccatgat
      361 tgtggccatc gcgacgcggc acgctgacgc agtcgacatt aagtttctcg tcgatgggcg
      421 taaagtctgg gtggcgctgc cgcatccggc gtgggtcgag tacaagaagc gcacccgcaa
      481 gctgattacc gattcgctgg cgcaagagat cgccgggcat tatctcaaat gggcactgga
      541 gtcaggcgag ggagtggggc gggagatgta ttcgctgacg gtcgaagaaa cgctggcgca
      601 tctcgacgca gtggtggcag agatgcaggc gcagccggca tagacaattg ttgggccggg
      661 aactatgttc ggtgcttcta ggctatgaaa cccaaatatt gatttctgct ccgatcaccc
      721 aaccgctgtc ccttttctcc atggccacat agtaacctcc gccacacagt ccgccgcaga
      781 ccttctcgaa atagaagagc gcctgcgtgc catcaacacc gaaccctact cgcgacaaag
      841 tcacgattcc actcgaaccc ggatattgct gataaaaggc gtcccaggat cccccgcctt
      901 tgcggaagat agatgtctcc ttggccgcgt caatcagttg gtaattaacg ggcaagcgaa
      961 aagaggagtg aacgagagcc tgctgcaaat tcgcccttag aaacgagtcc agtgtcgctt
     1021 gctgtagggc agggtatttg ccgcgcagaa actcggccgt tctcttccaa ggaagcaact
     1081 gtccgttgtc gtcgcttaag agatgacggt cgttacaccc ggtcgtattg aaaaacacaa
     1141 gtttcgagac gccgttgctg gccggcttcc cgctgaattt gccgtttgta tatgccgaaa
     1201 gtacgttgta ctcagccgca gttaagtcat cggcaagtgc tggcgaggac tccccgcgtt
     1261 tgcatgcgct taagccgacg accagtgaca gcaggacggc ggtgagagct ttatacatat
     1321 caggccgcat tgcaccacaa ggcgccggtg tgatgacagc agttaccact cggactcact
     1381 agatcaccac agcctctttg tactccccaa acacgcgccg catcgcatcg gaaatttctc
     1441 ccacggttgc cttggcttcg accgcagcca gaatccgcgg catcaggttc gcgccggaac
     1501 gcgcggcgtc ttccacgccg gccatcgccg tcttccagac gcccgcatcc cgccgcgcgc
     1561 gcagcgcacg cacgcgctcg atctgcttgg gctcgagcgc gggatcgatg cgctgaatgg
     1621 gaatcggctt ctcttcttcc acttcgaaac gattcacgcc cacgacgatc gcttcctgct
     1681 gatcgaccgc tcgctggtat tcgtaggctg cgttctgaat ttcctgctgc acgaagccgc
     1741 gttcgatcgc cttcagcatg ccgcccaaaa cttcgatctt gcctaagtac tcggccgcca
     1801 gcctttcaac ctcgttcgtc agggtctcga tgtaatacga ccccgccagc ggatcgacgg
     1861 tctgcggcgc gcccgattca taggcaatga tctgctgcgt tcggagagcg atgcgggcgg
     1921 cctgctcggt gggcagagca agggcctcgt caaaagaatt ggtgtgcagg gattgggttc
     1981 caccgagcac cgcagccagc gcctggatcg cggtgcgcac gatgttgttt tccggctgct
     2041 gcgcggtcag ggtcgatccg gcggtctggg tgtggaagcg cagcatccat gagcgcggat
     2101 ttttcgcctt gaaatgctcg cgcatgatgc gcgcccacat gcggcgcgcg gcacggaatt
     2161 tcgccacttc ttccagaaaa ttgctgtgcg cgttgaagaa gaacgagagc cgcggcgcaa
     2221 acttgtcgac atcaagcccg gcctcgagcg cagcatccac gtacgccatg ccgttgccca
     2281 acgtgaaggc gacttcctgc accgccgtcg atccggcctc gcgcatgtga tagccggaga
     2341 tggaaatcgt gttccagtcg ggcacgttct cgttggccca ggcgaacatg tcggtgatga
     2401 tccgcatggc ctgatgcggc gggtaaatgt aggtcccgcg ggcgatgtat tccttcagca
     2461 cgtcgttctg caccgtgcct gaaagttggc ggatatccgc gccctgccgc ctcgccacgg
     2521 ccacgtacaa ggcgagcagg atcgaggccg tagcgttgat cgtcatcgag gtagaaatct
     2581 tcgtcagatc gatcccgtcg aacaggcgtt gcatgtcctc gatggaatcg atcgcaacgc
     2641 cgaccttgcc gacttccccc gccgcgagcg cgtgatcgga atccatgccg atctacgtgg
     2701 gcagatcgaa ggcaaccgac aatcccgttg tgccgttggc cagcaggtac ttgtaacgtt
     2761 tgttcgattc ctccgcgtca cccatgccgg cgtactggcg catcgtccag aggcggccgc
     2821 ggtacatggt ggcctgcacg ccgcgagtga agggatattc tccgggatat cccacttcgc
     2881 tctcgtagtc agaacctttg agatcttcgg gagtgtagag gggcttgacc gggatgtgcg
     2941 aggacgtctg tggcgctaga ggatcagcgc cggacttgcc tgcgctcggc ttcattgccg
     3001 ga
//
LOCUS       DASPUL010000505         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1190869, whole genome shotgun sequence.
ACCESSION   DASPUL010000505 DASPUL010000000
VERSION     DASPUL010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190869"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VEJ00_11965"
     CDS             <1..1037
                     /locus_tag="VEJ00_11965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HXX01921.1"
                     /translation="VAAMKSIIGQANSSRSMLSRLPNLYGPQVGLLFVPLARFSFTTA
                     AWIWAVLSLPAFFICVYAIWKCCPVLHPHSGMVSTSALAFPPLFHFFVRGQLSALVLA
                     CFTAAFLAWRADRRWLAGVALGLLAFKPQFLVAVPLILLLSGAWRELTGLLASACAQL
                     ALARIYFGSAVMRAYIDTLFHFPRWIETAEPGIAHMQMHSLRSFWALLLPWPRTALAL
                     YVLAALAVVGSTVAIWKSASPLAARFSALTLAAVLINPHLFVYDLLVLAPMYLLLADW
                     TIANTGNSSAAAIHVLLYLAFVLPLFGPLSQWTHVQLSVPVLAALLWTLWRYSGTEGH
                     KLASNESSVV"
     gene            1062..>2834
                     /locus_tag="VEJ00_11970"
     CDS             1062..>2834
                     /locus_tag="VEJ00_11970"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXX01922.1"
                     /translation="MRIPKTAFLVVIVVTCALSILAPKVRAQNKPKLTLDDFFNSVSF
                     PAFEVSPDGNSVVIVTERADWDQQIFRTDLWLYRAGKSDAGKSDDGKAGSLIQLTQSG
                     HDSEPKWSPDGRWIAFLSERKSSSEKGDDSDADSDSKDEYSQIYLISPSGGEAFAVTE
                     GEESVHSFSWAPDGQAMYFATRQPWSKVQKDDYRENWKDVVQYRTAERGDTIFVIDLS
                     TALARHTSAPARVERREGEPEPEPDVTKGARAIASTPLRVGDMVTSPDGTRLAFVSNA
                     INQRAEKADDVEVYTVDVARALSSVKAASESKPTLIPPHRVTNNSASEGQLRWANDSR
                     HIFFTVEVGDVSGPYRDLQPHLYWVDSDSGAVEQWSKDFIGPVEHYAVTPDSVVASAR
                     VGTEVQMYSAARSADSLNRLSNWPGAYSQVSIGLHSPKVAFAYSALGKPEEIYLADSE
                     SQLGQARPITAFNKRIADSDLPQGKPYQWKADDGTTVEGMLIYPPGKFEQKHLPMFTL
                     IHGGPADADGNSFSADWYDWAALAATNGWLVFRPNYRGSTGYGDKFLTEIVPQIVSRP
                     GKDILEGVDALVKDDIADPDRLTVG"
BASE COUNT          517 a          925 c          785 g          607 t
ORIGIN      
        1 gcgtggctgc gatgaagtcg atcatcggac aggcgaattc gtcgcgctcg atgctttcgc
       61 gcctgcccaa cctctacgga ccgcaggtcg ggcttctctt cgtacctcta gcgcgattct
      121 ctttcactac cgccgcgtgg atctgggctg tgctaagcct gccggcattt ttcatttgcg
      181 tgtatgcgat ctggaaatgt tgtcccgttc ttcacccgca ctctggaatg gtcagcacct
      241 cggcgcttgc ttttccgccc ctgtttcatt ttttcgtccg tggacagcta tcggctcttg
      301 tcctggcctg ttttactgcg gcctttctgg cgtggcgcgc cgaccgccga tggctggcgg
      361 gagttgcgtt gggtctgctg gcgttcaagc cgcagtttct cgtggcggtc ccattgattc
      421 tgctgctgtc tggtgcatgg agagaattga ccggccttct ggcctcggca tgcgcgcagc
      481 ttgcattggc gagaatctat ttcggctccg cagtcatgcg ggcctacatc gacacactgt
      541 ttcacttccc gcgctggatc gaaaccgcgg agcccggcat cgcccacatg caaatgcact
      601 ccctgcgctc gttctgggcc ttgttgctcc catggccccg gaccgcgctc gctctgtacg
      661 ttctggctgc tctcgctgtg gtcgggtcga cggtggcaat ctggaaatct gcatctcctc
      721 tggctgcccg cttttctgcg ctgaccctgg ccgcagtcct gatcaacccg catttgtttg
      781 tctacgacct gctggtactg gctcccatgt atctgctcct cgcggactgg actatcgcca
      841 acaccggcaa ttcttccgcc gccgccatcc acgtgctgct gtaccttgcc tttgtgctgc
      901 ccctgttcgg gccgctgtcg caatggacgc atgtccaact ctcggttcct gtcctcgcgg
      961 cgctcctgtg gacactgtgg cgttattccg gaaccgaagg tcacaaactt gcttcgaatg
     1021 aatcctccgt tgtataaccc ttcttcccac cgggaacccc tatgcgaatc cccaaaactg
     1081 cgttcttagt tgtgattgtc gtgacttgcg cgctcagtat tctcgcaccc aaggttcgcg
     1141 cccagaacaa accgaagctg acgctcgacg acttcttcaa ctccgtgagc tttcctgcat
     1201 tcgaagtttc gcccgacggc aactcggtcg tcattgtcac cgagcgcgcg gactgggatc
     1261 agcaaatttt ccgcaccgat ctgtggctgt atcgtgccgg aaagagcgat gccgggaaga
     1321 gtgacgacgg aaaagccgga tcgttgatcc agttgacgca atccggtcac gattccgagc
     1381 ccaaatggtc gccagacggg cgctggatcg cgttcctctc cgagcgcaag tcttcttcgg
     1441 agaaaggcga cgattccgat gctgattccg actccaaaga cgagtacagc cagatctacc
     1501 tcatttcgcc cagcggcggc gaggcgtttg ccgtaaccga gggggaagaa agcgttcaca
     1561 gtttttcctg ggcgcccgat gggcaggcga tgtatttcgc tacccgccag ccgtggagca
     1621 aggttcagaa ggacgactac cgggaaaact ggaaagacgt cgtacagtac cgcacggccg
     1681 agcgcggcga cacgattttc gttatcgatc tttcaactgc gctcgcacga cacacctccg
     1741 cgccggccag ggtcgaacgg agagaaggcg agcccgagcc cgaacccgac gtgactaaag
     1801 gcgctcgcgc catcgcgagt accccgttgc gcgtggggga tatggttacc tcgcccgacg
     1861 gcaccaggct cgcgttcgtc agcaatgcca tcaaccagcg cgcggaaaaa gccgacgacg
     1921 tcgaagtcta tacagtcgat gtggcgcggg cgctctcgtc cgtgaaagcc gccagcgaga
     1981 gcaagcccac gctcatccct ccgcatcgcg tcaccaacaa ctcggcatcc gaaggccagc
     2041 tgcgctgggc caatgacagc cgccacatct tcttcacagt cgaagtcgga gatgtttccg
     2101 gtccctaccg cgacttgcag cctcatctgt actgggttga ttcggattcc ggtgcggtcg
     2161 agcaatggag caaggatttt atcggccccg tcgagcacta cgccgtcacg ccagattcag
     2221 tcgtagcgtc cgctcgcgtc ggcaccgaag ttcaaatgta ttccgccgcc agatcagccg
     2281 attcgctgaa ccgcctcagc aactggccgg gagcctactc gcaggtctcc atcgggctgc
     2341 actcgccgaa ggtcgccttc gcgtactccg cgctcggcaa gcccgaagaa atctacctcg
     2401 ccgacagcga gtcgcaactc ggccaggcgc gtcccatcac tgcattcaac aagcggattg
     2461 cggacagcga ccttccccaa ggcaagccgt accagtggaa ggccgacgac ggcacaaccg
     2521 tggaaggcat gctgatctat cctccgggca aattcgagca gaagcacctg cccatgttca
     2581 cgctgattca cggaggcccc gccgacgccg acggcaacag cttcagcgcc gactggtacg
     2641 actgggctgc gctcgccgcc accaacggat ggctcgtctt ccgccccaac tatcgcggtt
     2701 ctaccggata cggcgacaaa tttctcacgg agatcgttcc ccaaatcgtc tcgcgccccg
     2761 gcaaggatat tctcgaaggc gtcgacgcgc tggtgaaaga cgacatagcc gaccctgacc
     2821 gcctcaccgt cggc
//
LOCUS       DASPUL010000506         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1190891, whole genome shotgun sequence.
ACCESSION   DASPUL010000506 DASPUL010000000
VERSION     DASPUL010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190891"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VEJ00_11975"
     CDS             <1..409
                     /locus_tag="VEJ00_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01923.1"
                     /translation="QLANSGSVKSRLNNYFNGSCIDRQDLTKPLDVVGGTNVPVWPVI
                     DPSPGGRGTDFGNSAVGAVTGPGQQNYDFTLEKMFSVRESMNVQFRADFFNIFNHAQF
                     ANPDVGTADATLGQILSTTVNPRIVQFALKVNF"
     gene            412..1956
                     /locus_tag="VEJ00_11980"
     CDS             412..1956
                     /locus_tag="VEJ00_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01924.1"
                     /translation="MRKHSWTVRCALAAVGVLLCAELSIEARASDWPPPTDEPVITQH
                     QITLNGIVLKYTAHAGFLTLRDEFNKPHGRIFYVFYRADAATHKSPRPLTFAWNGGPG
                     SASSLLHIGALGPYRAKDLDEYSSTPPPYQLTENESTWLDETDLVFVDPVGTGYSYAV
                     EPTFGKEFWSPKGDIDSVAEFIRLFLTHYEYAAHTPIFLVGESYGTLRAAGVAGKLSD
                     KDFNIAGIVLISAALDLHETPSDLQFISLLPSYTAAAFVHKKLPSELQENLDAAIQKS
                     EEWAISRYSVALLQGDRLSSAERASVTKDFSRFTGLSEEYVASHNLKIGMEDFSGQLL
                     QDKKLVLGHYDVRATAASTSSEGEYNPTLDPSLNTHGTGTLIVPYLQSQLNVKTDAFY
                     AGPFGGRWPPPTAPRGDWMSVRWDWGEGSAVDGAAALTRALRANKSLQVLVISGLYDL
                     ATPFFGVENTFAHMGLDPDTRKRAQLVRYQAGHMVYLDRSNRRKLKQDFVELTKRALA
                     PAGSSK"
     gene            2043..>2278
                     /locus_tag="VEJ00_11985"
     CDS             2043..>2278
                     /locus_tag="VEJ00_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01925.1"
                     /translation="MVKRIAAILLFLMGLNAAATAQPGAATSTSPQLSLTLHPVLAPE
                     SKVSAIDVALDLSGIRADPGQPFSLRIPIEFAGV"
BASE COUNT          468 a          686 c          642 g          482 t
ORIGIN      
        1 ccaactggcg aactcgggat cggtcaaaag ccggctgaat aactatttca acggcagctg
       61 cattgatcgg caggatctga ccaaaccgct agatgtcgtt ggcggcacaa acgttccggt
      121 ctggccggtc atcgatcctt cgccgggagg ccggggcacc gattttggca acagcgcagt
      181 tggggccgta acggggccgg ggcagcagaa ctacgacttc acgttggaga agatgttttc
      241 cgttcgcgaa agcatgaacg ttcagtttcg ggcggatttc ttcaacatat tcaatcacgc
      301 gcaatttgcg aatcccgatg tcggcacggc cgatgcgact ctcggccaaa ttctttcgac
      361 gacagtcaac ccgcgaatcg tgcagttcgc gctcaaggtg aacttctagg catgcgcaaa
      421 catagctgga ccgttcgctg tgccctggca gctgtggggg tgctgctctg cgccgaattg
      481 tcgattgagg cccgcgcgtc cgactggccg ccgccgaccg acgaacccgt catcacgcaa
      541 caccagatca cactgaacgg aattgttctg aagtacacag cgcatgccgg ctttctcacg
      601 ttgcgcgatg agttcaacaa gcctcacggc cgcatcttct atgttttcta ccgtgcggac
      661 gccgctacgc ataaatcacc gcggccgtta acctttgcct ggaatggcgg cccgggatcg
      721 gcctcgtctc tgcttcacat cggcgctctg ggtccgtatc gcgccaagga tctcgacgag
      781 tattcctcca ctccacctcc gtatcaactc acggagaacg aaagcacctg gctggatgaa
      841 accgacctgg tattcgtgga tccggtaggc accggttaca gctatgcggt tgagccgacg
      901 tttggcaagg aattctggag tcccaaaggc gacatcgatt cggttgcaga gttcattcgc
      961 ctttttctga cgcactacga gtatgccgca catacgccca ttttcctggt cggtgagagt
     1021 tatggcactc tgcgtgccgc cggcgtcgcc gggaagctga gcgacaagga tttcaacatt
     1081 gccgggatcg tgctgatctc ggcggcgctc gatttgcacg aaacaccttc ggacctgcag
     1141 ttcatctcgc tgctcccgag ctataccgct gcggcattcg ttcacaagaa gctgccgtcc
     1201 gagctgcagg agaatctgga cgcagcaatc cagaaatcgg aggaatgggc gatctcgcga
     1261 tactcggtcg cgctgttgca aggagatcgt ctgtcgtcgg ctgaacgcgc cagcgtcacg
     1321 aaggacttct cccggtttac cggattgagt gaggaatacg tcgccagcca caatctgaag
     1381 atcgggatgg aagatttctc cggccagctg ttgcaggaca agaaactcgt tctcggtcac
     1441 tacgacgttc gcgccacggc ggcgagcacg tcctccgagg gcgagtacaa cccgacactc
     1501 gacccaagcc tgaatactca cggaaccggc acgcttatcg ttccttacct gcaatcccag
     1561 ctcaacgtca agacagacgc tttttacgcc ggtccttttg gagggcgctg gcctccaccc
     1621 accgcaccga ggggtgactg gatgtccgtg cggtgggatt ggggtgaggg gagcgccgtt
     1681 gacggcgcgg ccgccctcac tcgcgccctg agggcaaaca aatcgctgca ggtgctggtg
     1741 atttccggcc tctacgatct cgccacgcca tttttcggcg tcgaaaatac tttcgctcat
     1801 atgggcctcg acccggatac gcggaagcgc gcccaacttg tccgctacca ggctgggcac
     1861 atggtttatc tggatcggtc gaaccgaaga aagctcaaac aagactttgt cgagttgacc
     1921 aaacgcgccc tggcgccggc cggatcctcg aagtgatgcg ccccagtcag aactgatcgg
     1981 gcatctcttc cgcgttgcac gacgacattc caacgagcat ggaatgacga gatataaagt
     2041 ctatggtcaa aagaattgcg gcaattctgc tgttcctgat gggcctgaat gcggctgcca
     2101 cagcgcagcc cggtgcggca acttccacca gtccacaact tagcctgacc cttcaccctg
     2161 tcctcgctcc tgagagcaag gtctcggcca ttgacgtggc gctcgatctg agtggcattc
     2221 gcgccgatcc cgggcaaccg ttttcgctcc gcattccgat cgagtttgct ggcgtcaa
//
LOCUS       DASPUL010000507         5722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_774538, whole genome shotgun sequence.
ACCESSION   DASPUL010000507 DASPUL010000000
VERSION     DASPUL010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774538"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /gene="hflX"
                     /locus_tag="VEJ00_11990"
     CDS             <1..1452
                     /gene="hflX"
                     /locus_tag="VEJ00_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXX01926.1"
                     /translation="DPGREEPSGERAFLVGLEVRSRAARGAVTAQAAAAREAAQNAST
                     QIPTPSASSGQALSQKARKDGAPDASRVNGKPSIPEFDAEESLAELRTLAESAGAGVV
                     GQILQRRDRLDPATLIGAGKLEEIAGAAASVNADIVLFDHDLSPSQQRNIEKTVHRRV
                     IDRTQLILDIFARHARTREGQLQVELAQLQYLMPRLAGRGVEMSQLGGGIGTRGPGET
                     QLETDRRKIARRVRHVEQQLENVRRIRAQQRQRRESAPVATVALVGYTNAGKSTLFNA
                     LTRAGVLSSPKMFATLDPTIRAVSLPSKRKVLLSDTVGFIRSLPHTLVSAFRATLEEV
                     QRASLILHVSDASSRLSAEQDAQVGIVLKELEAEKRPRLRVMNKVDLLDEEVAASLMA
                     DASREGAKTLYVSAIDGMGLDRLLDRIDAMIEEDPVSRVHLRVPQKEGKMLALLEARA
                     RIYSRKYRDGAVDLEAEAPESVVRRVREWVVGS"
     gene            1593..2804
                     /locus_tag="VEJ00_11995"
     CDS             1593..2804
                     /locus_tag="VEJ00_11995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXX01927.1"
                     /translation="MRNVRTLIVAIVCVALSSAALAQGGSGPGALLWPTHGWAKDQPG
                     GVGLEEKMLAAFDADLAQNKYSLVDSFAVYRCGRQVFERKYSHDYGKIYGKEAHERGP
                     LNAHLTGPYNYFDPAWHPYYHGTELHSMQSVSKTVSSVIWGIAMTRGDFKAGLETPLL
                     KYFDEAKVKNVDERKRRMTLRHVLTMTTGLDWNEEVAYDDPKNDSSIMEATDDWVQYV
                     IDRPMAHEPGTVFNYSSGVSMFLAYVFEKETGQDIVRYGEKYLFGPLGIEYYWKRTPL
                     GVADTEGGLFLKNEDLAKIGYLYLHDGFWDGQKIVSKDWVKQSLAPATDAEEGFKYGY
                     KWWLLPRKDRPGYVWMARGFGGQRLMVFPEEELIAVFTGWEILKDEAASADLVDRILP
                     AVRTQNCGGSK"
     gene            complement(2955..3593)
                     /locus_tag="VEJ00_12000"
     CDS             complement(2955..3593)
                     /locus_tag="VEJ00_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01928.1"
                     /translation="MNRTLVLLLCLVAVIAVWFATSRVSSQPASASISLAPQVEGSKI
                     YFVPIGDFPSEQLDSLVQYYRQKYTLDISILKSITVDPSARDSSRQQLMAENLAAGLR
                     QAVPEYANDPKAILIGFTSEDMYPTSKGWQFAFGWRLGNARAAVVSTARLGFIYVGMP
                     LDLHVSDTRLRKVVSKDIGIMYYGLPSSTNPKSVLYNQIGGIQELDEMGEDF"
     gene            complement(3717..4451)
                     /locus_tag="VEJ00_12005"
     CDS             complement(3717..4451)
                     /locus_tag="VEJ00_12005"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXX01929.1"
                     /translation="MRIVVWNCRMALAKKRDLLYQLRPDIAVIPECSRASMNLCREDG
                     FDTRWWGGNKHKGLGVLAAKPYSLDSASRPGLSPRPSQPRQRWIAPVRVRGPLDFLLL
                     SVWACPVGTSREHNYVGQTYDAIVRHPRWFGETLPTVICGDFNSNPIFDPGRKKKTHS
                     AVVRLLADRGLASAYHEFFSEPQGAESRPTYYFWHRRERSFHIDYIFLPRTWMNRVRD
                     FEVGSYSQWRPASDHVPIVVEIADAA"
     gene            4858..5313
                     /locus_tag="VEJ00_12010"
     CDS             4858..5313
                     /locus_tag="VEJ00_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01930.1"
                     /translation="MDQTLHQLGELLLGAVPTVILLALLYTLYTFIVHKPLKRVLEER
                     RSKTEGAVEKSRADIARAEARTAEYEQKLREARAAIFRSQEARRQAAAQARAKAVAEA
                     HGRAQAQVQAAKKGIESDRVAAQAGLQAEVAALAREIVRRVLEPAGAGR"
     gene            5332..>5722
                     /locus_tag="VEJ00_12015"
     CDS             5332..>5722
                     /locus_tag="VEJ00_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01931.1"
                     /translation="MNSKTTILWRFGLALLLVSCSGLTLVRAQDQSSSANQKQEVSDS
                     SQQSAGPGKQLAHESREAAGEEKDETAEFKQSASVRLISKWTGLDLQRSYWLSVITNF
                     AVVAAVIIWAGRKYLPGMLQARTAAIQK"
BASE COUNT         1168 a         1497 c         1979 g         1078 t
ORIGIN      
        1 gaccccgggc gcgaagagcc gagcggagag cgcgcgttcc tggtcgggct ggaggtgcgc
       61 agtcgcgcgg cccgaggtgc ggtcacggcg caggcagcgg ctgcgcgcga agcggcccag
      121 aatgcttcaa cacaaatccc caccccttcg gcaagctcag ggcaggctct ttcgcaaaaa
      181 gcgcgaaagg atggggcacc cgatgcgtcg agggtcaacg gaaaaccaag tattccggag
      241 tttgatgccg aggaatcgtt ggctgaactg cgcacgctgg ccgagagtgc cggagccgga
      301 gtggtcggac agatcctgca gcggcgcgat cgactcgatc ccgcaacctt gatcggcgcg
      361 ggcaagctgg aagaaatcgc gggcgcggca gcctcagtga atgcggatat tgttctcttc
      421 gaccacgacc tcagcccatc gcagcagcgc aacatcgaaa aaacggttca ccggcgggtg
      481 atcgaccgca cccagctcat cctcgacatt tttgcgcggc acgcccggac gcgggaaggc
      541 cagctgcagg tggagctggc gcaattgcag tatctgatgc cgcggctggc cgggcgtggc
      601 gtggaaatgt cgcagctggg gggcggcatc gggacgcgcg ggccgggcga aacgcagctc
      661 gagactgacc ggcggaagat tgcgcggcgc gtgcggcatg tggagcagca actggagaac
      721 gtgcggcgca tccgggcgca acagcggcag cggcgggagt cggcgccggt ggcgacggtc
      781 gcgctggtgg gatatacgaa cgcggggaag tcgactttgt tcaacgccct gacgcgggcg
      841 ggggtgctgt cgtcgccgaa gatgttcgcc acgcttgatc cgacgattcg cgcggtgagc
      901 ctgccgtcga agaggaaagt gctgctgtcg gatacggtgg gattcatccg cagcctgccg
      961 cacacgctag tgtcggcgtt tcgcgcgacg ctggaagaag tgcagcgtgc ttcgctgatt
     1021 ctgcacgttt cggatgcgag cagccggctg tcggccgagc aggacgcgca ggtgggaatc
     1081 gtgctgaagg aactggaagc ggagaaaaga ccacgcctgc gtgtgatgaa taaagtggat
     1141 ttgctggatg aggaagtggc ggcgagcttg atggcggatg cgtcgcgcga gggtgcgaag
     1201 actttatatg tttcagcgat tgacggtatg gggctggatc ggctgctgga ccggattgac
     1261 gcgatgatcg aagaagatcc ggtgagccgg gtgcatctgc gcgtgccgca gaaagaaggg
     1321 aagatgctgg cgctgctgga ggcgcgggca cggatttatt cccggaagta tcgggatggc
     1381 gcggtggatc ttgaggcgga ggcgccggag tcggtggtac gaagggtgcg ggagtgggtt
     1441 gtcggttcct gaaagcccca cttctcgcac agaacgcgag aaatggggca cccagcacag
     1501 cagtgattgc atcttgggct gctgtgcggc ggaagtggag ttcgatgctt ttgtctgcaa
     1561 cctacattcg ccggcactct tcagaggaca acatgaggaa cgtgaggacg ctaatcgttg
     1621 ctatcgtttg tgtcgcgttg tcgtccgcgg ctctggcgca gggtggttct ggacctggcg
     1681 cattgctttg gccgactcac ggctgggcca aggatcagcc ggggggtgtt ggcctcgagg
     1741 aaaaaatgct ggcggcgttt gatgcggatc tggcgcagaa caagtattcg ctggtcgaca
     1801 gctttgctgt gtatcgctgc ggaaggcagg tgttcgagcg gaagtattcg cacgactacg
     1861 gcaagattta tggcaaagag gcgcacgagc gcgggccgct gaatgcgcac ctgacagggc
     1921 cctataacta cttcgatccg gcgtggcacc cgtactatca cggcactgag ctccacagca
     1981 tgcagtcggt gagcaagacg gtgtcgtcgg tgatttgggg gatcgcgatg acgcgcgggg
     2041 acttcaaggc cggcctcgag acgccattgc tgaagtattt cgacgaggcc aaggtgaaga
     2101 acgtcgacga gcgcaagcgg cgcatgacgc tgcggcacgt gctgaccatg accactgggc
     2161 tcgactggaa tgaggaggtg gcctacgacg atcccaagaa cgactcgagc atcatggagg
     2221 cgactgacga ctgggtgcaa tacgtgatcg accggccgat ggcgcacgag ccggggacag
     2281 ttttcaacta cagcagcgga gtctcgatgt ttctggctta tgtcttcgaa aaggaaacgg
     2341 ggcaggacat cgtgagatac ggagagaaat atctgtttgg gccgctgggt atcgagtatt
     2401 actggaagcg gacgccgctg ggcgtagccg acaccgaggg tggactgttt ctgaagaatg
     2461 aagatctggc caagattgga tacctgtatc tgcatgacgg cttttgggac ggccagaaga
     2521 ttgtttcgaa ggactgggtg aagcagtcgc tggccccggc gaccgacgcg gaggaaggct
     2581 tcaagtacgg atacaagtgg tggctgctgc cgcgcaagga tcggccggga tatgtgtgga
     2641 tggcgcgtgg gtttggcggt cagcggctga tggtgtttcc agaagaagag ctgattgccg
     2701 tgtttacggg gtgggagatt ctgaaggatg aggccgcgag cgcggacttg gttgaccgga
     2761 tacttcctgc agtgcggacg cagaactgcg gcggttcaaa gtgagctttc acggctgtta
     2821 gccatttcgc ggaatcgcgc caacaacgcg caccccagcg gctaaagccg cgcattgtcc
     2881 gggctcgatc ggcgcgcctg aaggcgcgcc ctttcaaaac taattcctga tggctaggcg
     2941 gggcggcgga cgtgctagaa gtcctctccc atttcgtcga gttcctgaat ccccccaatc
     3001 tggttgtaga gcacgctctt tgggttcgtg cttgagggca gcccgtaata catgatgccg
     3061 atgtccttgc tgacaacctt gcgcagacgc gtgtcggaca catgcagatc gaggggcatt
     3121 cccacataga tgaaccccag gcgagcggtc gaaaccactg cagcccgcgc gttgcccagt
     3181 ctccagccga aggcaaactg ccagcccttc gaagtcggat acatgtcttc ggaggtgaag
     3241 ccgatcagga ttgccttggg gtcgttcgca tattcgggca cggcctgccg caagccagcc
     3301 gccagattct cggccatcaa ttgctgacgc gaggaatctc tcgcagaagg gtcgacggta
     3361 atgcttttca gaatagaaat atcgagggtg tatttctggc gatagtactg aaccagagag
     3421 tcaagctgct cgctggggaa atctccgatc gggacaaagt agatcttgct gccttcgacc
     3481 tgaggggcga gcgatatcga tgcactggca ggttgggatg acactctcga agtggcgaac
     3541 cagacggcga tgacggcgac caggcagagc aacagaacta atgtacgatt cacaactact
     3601 ccctttgtca ggcatcgtgc agtcgcaggg gatagatggg attacaacac ggatgtgggc
     3661 tgcgactgga gtaaagggaa cgcaacaagg ttaccaaggt tccgattcac ggatcgtcag
     3721 gccgcgtcgg cgatctcgac gacgatcggg acgtgatcgc tggcgggtct ccattggctg
     3781 tagcttccga cttcgaaatc tctcacgcgg ttcatccagg tgcgcggcag gaagatgtag
     3841 tcgatgtgga agcttcgttc ccggcgatgc cagaagtagt acgtcgggcg gctttcggcg
     3901 ccttgcggct cggagaagaa ttcgtggtag gcgctggcca ggccgcggtc ggcgaggagt
     3961 cgcacgacag cggaatgggt tttcttcttg cggccgggat cgaagatcgg gttgctgttg
     4021 aagtcgccgc agatgaccgt gggaagggtt tcgccgaacc acctgggatg gcgcacgatg
     4081 gcgtcgtaag tctgccccac atagttgtgt tcgcgactgg tgccgaccgg gcacgcccac
     4141 accgaaagaa gcaggaagtc caacgggccg cgcacacgga ccggtgcgat ccatctttgg
     4201 cggggctggc tcggtcgggg cgagagtccc ggcctggatg ccgagtccag gctataaggc
     4261 ttggccgcca atactccgag tcctttgtgt ttgtttcctc cccaccagcg ggtgtcgaat
     4321 ccgtcttccc tgcataaatt catggaagcg cgggaacact cggggatcac cgcgatgtcc
     4381 gggcgaagct ggtagagcag atcccgcttc ttcgccaggg ccatgcggca gttccacacc
     4441 acgattcgca tcgagggatt acgttcttga caatgttgtc aagggtagca aattaaatgg
     4501 gccggcaacc aagaagcggt gacattcctt ggctatcggc ccattcgatc ctgaattgga
     4561 tcggaggtga tacctctatg ctagtccttt gggcagggaa gtcaaggaca atatgtgcag
     4621 aggcctgcat ggacggcgga gggctaatcg gggcaaaaac cccactggga ggccctttcc
     4681 cggcacccca aggctcttcc cggaatgcgc aagaagggcc gatcccaagc ttttcgaagc
     4741 ggtgctttgc gttgacatta aagtctgtgg gacgttagac ttatcagttt ccaaagccct
     4801 cattttgctg gctggctcgg cagtcgcacc tcagcaaaaa catagttcag cgactcaatg
     4861 gatcagacgc tccatcaact aggggaatta ctgctcggag cggtgccgac ggtcattctg
     4921 ctggcgctgc tgtacacgct ctacactttc attgtgcaca agcccttgaa gcgggtgctg
     4981 gaggagcggc gcagcaagac cgaaggcgcg gtggagaaat cgcgggcgga tattgcccgg
     5041 gcggaagcgc gcacggccga gtatgaacag aagttgcgcg aggcccgggc agcgattttc
     5101 cgttcgcagg aagcgcggcg ccaggcggcg gcgcaggcgc gggccaaggc cgtggccgag
     5161 gcccacggaa gagctcaggc ccaggtgcag gctgcgaaaa aaggcattga gagcgaccgg
     5221 gttgcggccc aggcgggttt gcaggccgag gtagcggcgc tggcacggga gatcgtgcgg
     5281 cgcgtgctgg agccggcggg agcagggcga tagttgatct tccgatctct tatgaattcc
     5341 aagacgacga ttctgtggag gtttggtctg gcactgctgc tcgtgagctg cagcgggttg
     5401 acgctcgtcc gcgcgcagga ccaatcaagt tccgcgaatc agaagcagga agtgagcgat
     5461 tcgtcgcagc aatcggcggg gccgggcaag caactggcgc atgaatcgcg cgaggcggcc
     5521 ggcgaagaga aggatgagac ggccgagttc aagcagtcgg cgtccgtgcg gctgatctcg
     5581 aagtggaccg ggctggacct gcagcgctcg tactggctca gcgtgattac gaatttcgcg
     5641 gtcgttgccg cggtgatcat ctgggccggg cgcaaatacc tgccgggaat gttgcaggcc
     5701 cggacggcgg cgatccagaa gg
//
LOCUS       DASPUL010000508         3022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_504071, whole genome shotgun sequence.
ACCESSION   DASPUL010000508 DASPUL010000000
VERSION     DASPUL010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504071"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VEJ00_12020"
     CDS             complement(<1..1126)
                     /locus_tag="VEJ00_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX01932.1"
                     /translation="MDGSLQDSRYALRQLRKSPGFTAVAVLTLALGIGVSTAMFGVMN
                     AVLLQPPRFRDPDRIVRILSTQGDTVGGPSPLDVRDFAAQSHTFEKMAVYDSGWRKNV
                     GALPGSTEPEQLPIGLMPAAYFEVLGVTPLMGRLFTEDENRWGNHYEVILSYGFWQAR
                     FQGDPAVLGKSIRINDEPYTIIAVMPAGSPEWSFDSLRGPVELWTPFVPYLAANETSI
                     WDETARRSRAWAIGRLKPGVSLEEAQADLQRIAANLAASHPLDRGVSVALRPLQEDRV
                     GTLRPVVLLLMGAVILILLIACSNVANLLLARNSSRAREVAVRVAMGAARSVLVRQFM
                     TESLVLGVLSGALGCVLAWSSCAVVARAHPAQLVQLAGVRV"
     gene            complement(1136..>3022)
                     /locus_tag="VEJ00_12025"
     CDS             complement(1136..>3022)
                     /locus_tag="VEJ00_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX01933.1"
                     /translation="PIGEETEPAFFLRDSHWGTDAIGLLKPGVTLEQARDDMKRMNAG
                     LADAYPDVDGNIKANLIPLQDEIIGDMRPALLVLLGAVGFVLLIACVNVANLLLARST
                     ARHREFSVRVALGASHARVVRQLLTESILLSLSGGMLGVLLAKWGTAAAIAAVPQTVP
                     RAEEIGLDVRVLLFTLSISILTGIVFGLLPAIRTARTDISERLKDSGRTISSSRSRMQ
                     SALVMGEMAMALVLLVGAGLMCRTLISLWKVDPGFDPHGLSRFGVRPAPSLASQSQES
                     IRAALRQIEGTIRSVPGVEALSLHDGATPMEGDNEAGFLLDGQELPTQRAIYVPQALE
                     YSVEPDYLNTLRIPLLRGRFLSDEDNERSARVVVIDTSFAQKYFAGQDPIGKHIRVFD
                     VDSDSNRRVEVPLTIVGVVGHVNQWGLADDATQSLQPEMYRAIMQSEKAEVKNLAQGF
                     SVFLRSRTTPGSQAFFEAIRQKLVGSNADLIVSDYKAEDRVVGESIASQRFSLILLGA
                     FAGLALLLASIGIYGVLSYLVGQRTREIGVRMALGAQRLDVLRMVLRDGARMAMVGAG
                     IGLAAAFALTRLMASMLFGVKPTDPVTFGSVVAVLGAIALVACYVPARRAAKVDPMVA
                     LRYE"
BASE COUNT          655 a         1003 c          841 g          523 t
ORIGIN      
        1 ccacgcgaac gcccgccagt tgcaccaact gcgccggatg cgcgcgggcc acaaccgcac
       61 acgacgacca ggccagcaca cagcccagag caccgctgag gacgcccagc accaggctct
      121 cggtcatgaa ctgccggacc agaaccgatc ttgctgcgcc catcgcaact ctgaccgcca
      181 cttcgcgcgc ccggctggaa ttccttgcca gcaggagatt ggcgacattc gagcacgcaa
      241 tcagaaggat cagaatcacg gcgcccatca gcagaaggac gaccggacgc aacgtgccga
      301 cgcgatcctc ttgcaaggga cgaagagcca cactcacgcc gcggtcgagc ggatggctcg
      361 cagccaggtt ggccgcgatt ctctgcaaat cagcctgagc ctcttccagg ctgacgccgg
      421 gtttcagtcg gccgatggcc caggcgcgcg atcttctcgc tgtctcatcc caaatcgagg
      481 tttcgttcgc ggcaagatac ggaacgaacg gggtccacaa ctcgaccggc ccgcgtaagc
      541 tgtcgaagga ccattcgggc gatccggctg gcatcaccgc gatgatggta taaggctcgt
      601 cgttgatccg gatgctcttg ccgagaaccg cgggatcgcc ttgaaaccgc gcttgccaga
      661 atccatagct caagatcact tcatagtgat tgccccagcg gttctcgtcc tcggtaaaca
      721 gacgccccat caggggtgta acacccaaga cctcaaagta agcggcgggc atcaggccaa
      781 ttggaagctg ctccggctcg gtcgatcctg gaagggcgcc gacgttcttc ctccagcccg
      841 aatcgtagac agccatcttt tcgaaggtgt gactctgcgc ggcgaaatca cgcacatcga
      901 ggggcgaagg acctcccacc gtatcgccct gagtggaaag gattctgacg atgcggtcgg
      961 gatcgcgaaa ccgcggcggc tgcagcagaa ccgcattcat caccccgaac atcgctgtgc
     1021 ttaccccaat gcccagcgcc agcgtcagca ctgctacagc cgtaaacccc gggctcttgc
     1081 gcaactggcg cagcgcgtag cgactatctt gaagcgagcc atccatgttt cagtcctatt
     1141 cgtaccgcag cgccaccatg ggatcgacct tggccgcccg ccgcgcggga acgtagcaag
     1201 caaccagcgc aatcgcgcct aagaccgcta ctaccgaacc aaacgtgacg gggtcggttg
     1261 gcttcactcc aaacagcatg ctggccatca gtcgcgtgag cgcgaaggct gccgcgagcc
     1321 caatccctgc tcccaccatc gccatgcggg cgccgtcgcg cagcaccatc cgcaacacat
     1381 caagccgctg cgcacccagc gccatgcgca cgccgatctc acgcgtgcgc tgaccgacca
     1441 gatacgacag cacaccataa attccgatgc tggccagcag cagcgccaat ccggcaaatg
     1501 ctccgagcag gatgagcgaa aaacgctgac tggcgatcga ttcccctacc acccgatctt
     1561 cggctttgta gtcggaaaca atcaggtccg cattgcttcc cacgagcttc tggcgaatcg
     1621 cctcgaagaa agcttgggaa cccggtgttg tcctggaacg caaaaatacg ctgaacccct
     1681 gcgccaggtt tttcacttcc gctttttcgc tttgcatgat ggcccggtac atctcaggct
     1741 gtagggattg agtggcgtcg tcggccaacc cccattgatt cacatgccca accacaccca
     1801 cgattgtcag cggaacctca acccgtcgat tggaatcgct gtccacgtcg aacacgcgaa
     1861 tgtgcttgcc gatgggatcc tgaccggcga aatatttctg cgcgaagctt gtatcgatca
     1921 ctacgacacg agcggaccgt tcgttgtcct cgtcactgag gaagcggccg cgcagcagtg
     1981 gaatccgcaa cgtattcaga tagtccggtt ccacgctgta ctccaaagct tgcggaacat
     2041 agattgcgcg ctgcgtgggc aattcctgac cgtcgagcag gaagcccgct tcgttgtcgc
     2101 cttccatcgg tgtcgcgccg tcatgcagcg acaaggcttc aacgcccggc acgctgcgga
     2161 tggtcccctc gatttgccgc agcgcggcgc ggatcgactc ttgcgattga ctggccagag
     2221 aaggcgctgg cctcacgcca aaacgagaca ggccgtgtgg atcgaatccg ggatcgacct
     2281 tccacagcga gatcaaagtg cggcacatga gccccgcgcc caccagtaat accagcgcca
     2341 tcgccatctc acccatcacc agcgcagatt gcatgcgaga ccgagaactg gagatcgttc
     2401 gcccgctgtc cttgagtcgt tcgctgatgt cggtacgagc tgtgcgtatc gcaggcagca
     2461 ggccgaacac gatcccggta aggatcgaaa tcgatagagt aaacaagaga acccgaacat
     2521 cgagtccgat ctcctcggct cgcggaacgg tttgcggcac agccgcaatc gccgccgctg
     2581 ttccccattt ggcgaggagc acgccgagca taccgcctga cagcgacaac aggatgctct
     2641 cggttagaag ctggcgaact acgcgcgcat gactcgcacc gagtgccaca cgaaccgaga
     2701 actcgcgatg acgcgccgtc gaccgcgcca gaagcaagtt ggccacgttc acgcaggcga
     2761 tcagcaatac gaaccccacc gctcccaaca gcaccagcag cgccgggcgc atgtcgccga
     2821 tgatctcgtc ttgcaagggg atgaggttgg ccttgatatt gccgtcaacg tcgggatagg
     2881 catcggccag gccggcattc atccgcttca tatcgtcgcg cgcctgctcc agcgtgaccc
     2941 caggcttcag caagccaatg gcgtccgtgc cccaatggga atccctcagg aaaaaggccg
     3001 gctcggtttc ctccccgatg gg
//
LOCUS       DASPUL010000509         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1463199, whole genome shotgun sequence.
ACCESSION   DASPUL010000509 DASPUL010000000
VERSION     DASPUL010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463199"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1461
                     /gene="kynU"
                     /locus_tag="VEJ00_12030"
     CDS             175..1461
                     /gene="kynU"
                     /locus_tag="VEJ00_12030"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HXX01934.1"
                     /translation="MNAASFQPGSDFAATMDARDPLAHFRARFHFPKTQSGEDCIYLA
                     GHSLGLQPKAAREYLEQELNDWAHFGVEGHFHARNPWVPYHRLLTQQTAELVGAQPSE
                     VVVMNSLTVNLHLMMASFYRPTATRNKILVEGGAFPSDQYAVKSQIRFHGFDPASSLL
                     ELTPREGESCLRDDDITSLIEREGASIALILLGGVNYVTGQAFNLEEITRAGHLQECV
                     VGFDLAHAAGNLPLRLHDWGPDFAAWCSYKYLNGGPGCVAACFVHERHARAWHLPRFA
                     GWWGHDEKTRFQMGPDFHPMAGAEGWQLSNPPILALAPLRASMEIFAEAGMDRLRGKS
                     IALTGYMEFLLQQSRLKRPSPQFTIITPPEPERRGAQLSIRLPHEGREVCAKLAAEGV
                     IGDWREPDIFRVAPMPLYNSCQDVYRFVERFLATLT"
     gene            1522..>2088
                     /locus_tag="VEJ00_12035"
     CDS             1522..>2088
                     /locus_tag="VEJ00_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01935.1"
                     /translation="MRTYNRSVGKSPLQHARTWTVVVCAVVCVHIVLSVLAPRGFALT
                     AFGDLLQCAVLLCGTVTIAANVKVASRRARLFWVLMGLGVGMWFGAQLLWTYFEVLLR
                     QEAPNPFVGDVILFLHVVPMMAAVAIQPHVRRDDRALRVGTLDFLLLLTWWLFLYLFI
                     VIPWQYVSPTESTYGLSFDILYVCEQLVL"
BASE COUNT          357 a          656 c          604 g          471 t
ORIGIN      
        1 gtacgccatg gtcacgtttc accggatacc ctatgcggtt gcgctcgagc gcggacacac
       61 gcaggatcgc atccttaccg aattgtgcga ccccgtcagc cgcatggatg atctcgactg
      121 gggcaaggcc gaccgcctga ttcgcgctca acttaccctg ctggagctcg ccgcgtgaac
      181 gctgcttcat tccagccggg cagtgatttt gctgcgacga tggacgcccg cgatcccctc
      241 gctcactttc gcgcccgctt ccattttcca aaaacgcaga gtggtgagga ctgcatctat
      301 ctcgccggtc actccctcgg cctgcagccg aaagccgcca gggaatatct cgagcaggaa
      361 ctgaacgatt gggcgcattt tggcgtggag ggccactttc acgctcgtaa cccgtgggtg
      421 ccctatcacc ggcttctgac tcagcagacc gcggaactcg tcggtgcgca gccgtcagaa
      481 gtcgtagtga tgaattcgct gacggtcaac ctgcacctga tgatggcgtc gttttatcgc
      541 ccgaccgcga cgcggaataa gattctggtg gaaggcggcg catttccttc ggatcagtat
      601 gccgtgaagt cacagatccg tttccacggc ttcgatcctg cgtcgtctct gttggaactc
      661 acgccgcggg aaggcgaatc ctgccttcgc gatgacgaca tcacttcgct gatcgaacgc
      721 gaaggagctt cgatcgcgct gatcctgctg ggcggcgtga actacgtgac cggacaagcc
      781 ttcaacctgg aagaaatcac ccgagccgga cacctccagg aatgcgttgt gggcttcgac
      841 cttgcccacg cggcgggaaa tctgccgctg cgcctgcatg actgggggcc tgatttcgcc
      901 gcgtggtgca gctacaaata tttgaatggc ggtcccggct gcgtggcagc atgctttgtc
      961 cacgagcgcc acgcccgcgc ctggcatctc ccacgcttcg ccggctggtg gggtcacgat
     1021 gaaaaaacgc gcttccagat gggccccgat tttcatccca tggccggcgc cgaaggctgg
     1081 cagctcagca atccgccgat tctcgcgctc gcccctctgc gtgcctcgat ggagattttc
     1141 gccgaggctg gcatggaccg attgcgcgga aagagtatcg ccctcacggg gtacatggag
     1201 ttcctgctgc aacaatctcg actcaagcgg ccctcgccgc aattcactat catcaccccg
     1261 cccgagcccg agcgcagggg agcgcaactc tccatccgcc tgccacacga ggggcgtgaa
     1321 gtctgcgcga aactcgccgc cgaaggggtg atcggcgact ggcgcgagcc ggacatcttc
     1381 cgcgtcgctc ccatgccgct ttacaactca tgccaggacg tttaccggtt cgtggagcgt
     1441 ttcctcgcca ccctgactta ggcctgagat gtccactttc ggtcagttct cgcgttactt
     1501 ccgtaacaaa tttgcgggac ggtgcgaacc tacaatcgga gcgtggggaa gtctccgctc
     1561 caacacgctc gcacgtggac agtcgtagtg tgcgcagttg tgtgtgtcca cattgttttg
     1621 tctgtcctgg ctccgcgcgg ctttgccctc acagcgtttg gcgaccttct gcagtgcgcc
     1681 gttttgcttt gtggcacagt tacgattgcc gccaacgtaa aggtagcgtc tcgcagggcg
     1741 cgcctgttct gggtgctcat gggcttgggc gtcggaatgt ggtttggcgc ccagttgctc
     1801 tggacatact ttgaggtgtt gctgcggcag gaagcgccca atccctttgt cggcgacgtc
     1861 attctctttc tgcacgtcgt gcccatgatg gctgccgtgg ccattcagcc gcacgtgcgg
     1921 cgagatgatc gagcactccg cgtaggtact ctcgacttcc tgctgctgct cacctggtgg
     1981 ctgttccttt atctgttcat cgtgatcccc tggcagtatg tttccccgac cgaatctacc
     2041 tacggactca gctttgacat tctctacgtc tgcgagcagc tggttctg
//
LOCUS       DASPUL010000510         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1595375, whole genome shotgun sequence.
ACCESSION   DASPUL010000510 DASPUL010000000
VERSION     DASPUL010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595375"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VEJ00_12040"
     CDS             <1..501
                     /locus_tag="VEJ00_12040"
                     /inference="COORDINATES: protein motif:HMM:NF019307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HXX01936.1"
                     /translation="ERDESLNFLLRDTISLTMMGGSEQTNVIPPEAWANLDVRILPGG
                     DPKAVLEAVRRVVNDPDVTVEPLNAEFRVANYSGTDNALYTAIREVSAKYFPGTPVVP
                     HITSGYTENQRYRPLGIVAYGFNPYTATEDEGNSEHGNDERIRVEEVRRGPRILFDVV
                     AAVAGK"
     gene            complement(554..1669)
                     /locus_tag="VEJ00_12045"
     CDS             complement(554..1669)
                     /locus_tag="VEJ00_12045"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HXX01937.1"
                     /translation="MLAAYAVHVERSRGRRFVEPQHAYRNDFQRDRDRVIHARAFRRL
                     EAKTQVFTRRYSDHFRNRLTHTLEVSQISRTIAGALALNVDLAEALALVHDIGHPPFG
                     HAGEKALDAAMRAHGLSFDHNLHALRIVEDFEQRYAAFRGLNLTFEVREGIIKHSRDY
                     DAKDHPELAEYLLDQRPPLEAQLIDLTDEIAYNTADLDDGYEAHLLSLGEIREGVSVF
                     ERFYREVELIYPDAPHKLKFNETLKRVLNRFVDDLITNTQARLIRAAINSVEDVRSHG
                     ERLAAFSAAVDAERKQSKDFLYENLYYSPALSEEKDDAERVVRELFAFWMDHPGALPP
                     NYQEKAKDESLPRVICDYIAGMTDNFIVEQYEKHCGE"
     gene            1912..>3001
                     /locus_tag="VEJ00_12050"
     CDS             1912..>3001
                     /locus_tag="VEJ00_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HXX01938.1"
                     /translation="MLHVGLDVGSVSVKLAALGAREDSAVLSLLAETSSAFFAPRLPA
                     ANGWAERPVVCSAYRRIQGNPLQAASELLREFYAIVPQESISGIRFTGSGGRTVAAAF
                     EAEFENDFRTLVRCIRALYPQVRTVFEMGGEASKYIRLSDVGSSSNLGVLDYQSSTEC
                     AAGTGSFIDQQASRLRYDVEDVGEAACHASCAARVAGRCSVFAKTDMIHAQQKGYTAD
                     QILRGLCDAVARNFKSSIVKGRQIATPVAFVGGVALNAGVYEALREAFGLRESDCLVP
                     DLNCWMPALGASICGVEAGAKRTTVALHQVPPQIAAGEEIPTTGPLSLNNVTLLNRAP
                     AVALPQGAGCVDAYLGIDVGSVSTNLVVL"
BASE COUNT          579 a          806 c         1032 g          584 t
ORIGIN      
        1 gagagagacg agtcgctcaa cttcctgctg cgcgacacga tttcgctgac catgatgggc
       61 ggatcggagc agacgaacgt gattcccccc gaggcgtggg cgaatctgga tgtgcgcatt
      121 ctgcccggcg gcgatcccaa ggcggtgctg gaggcggtgc ggcgggtggt gaatgatccg
      181 gacgtgacag tcgagccgct gaacgccgag tttcgcgtgg caaattattc aggcacggac
      241 aacgctctct atacggcgat tcgcgaagtg tcggcaaagt attttcccgg gacgccggtg
      301 gtgcctcaca tcactagcgg atatacagag aatcagcgct accgcccgct ggggatcgtg
      361 gcctatgggt tcaatccgta cacggcgacc gaggatgaag ggaactccga gcacgggaac
      421 gacgagcgaa tccgcgtgga ggaagtgcgg cgggggccgc ggattctgtt tgacgtagta
      481 gcggcggtgg cggggaagta ggacctattt tcgccgccga tcgacgcgga cttgcgcgga
      541 tcttctatca aggtcattcg ccgcaatgct tctcgtattg ctcgacgatg aagttatcgg
      601 tcatgccggc gatgtagtcg cagatgacgc gaggcagcga ctcgtccttg gctttttcct
      661 gatagttcgg gggcagggcc ccggggtggt ccatccagaa ggcgaatagc tcgcggacga
      721 cgcgttccgc gtcgtctttt tcctcggaca aggccgggct gtagtagaga ttttcgtaca
      781 ggaaatcctt gctctgcttg cgctcggcgt cgaccgcggc gctgaaggcg gctagccgct
      841 cgccgtggct tcggacatcc tcgaccgaat tgatcgcggc gcgtatcagt cgtgcctggg
      901 tgttcgtaat cagatcgtcg acgaaacggt tgaggacccg cttcagggtc tcgttgaact
      961 tcagcttgtg gggagcatcg ggataaatca gttcgacttc ccgatagaag cgctcaaaaa
     1021 ccgaaacccc ttcacggatt tctcctaacg acagtagatg ggcttcatag ccgtcgtcga
     1081 ggtcggcggt gttgtaagcg atctcgtcgg taaggtcgat aagctgggcc tcgagagggg
     1141 gacgctggtc gagcaggtat tcagccagct caggatggtc tttcgcgtcg tagtcgcggg
     1201 agtgcttgat gatgccctcg cggacttcaa aggtgaggtt caggccacga aaagcggcgt
     1261 agcgctgctc gaaatcttcg acgatgcgca aggcgtgcaa gttgtggtcg aaggaaagac
     1321 cgtgggcgcg catggcggca tccagcgctt tttcgccggc atggccgaac ggagggtgac
     1381 cgatgtcgtg caccagagcc agcgcttcgg cgagatcgac attcagtgcg agcgctccgg
     1441 cgatggtgcg cgaaatctgc gagacttcga gcgtgtgggt caggcggttg cggaagtgat
     1501 cggaatagcg gcgggtgaaa acctgcgttt tggcctcgag gcggcggaag gcgcgggcgt
     1561 gaatcacgcg gtcgcggtcg cgctggaagt cgttgcggta cgcgtgctgc ggctcgacga
     1621 agcggcgtcc acgggagcgt tcgacgtgca ccgcgtaggc ggccagcatg agggaagcat
     1681 agcagagtaa cgagtgctgg atgggggggc aggagcgcaa tcatctcggc ggttcgcgta
     1741 gaaagagccc ggggagcggg aggtaagaag tgatccgaat cacagacctt gatcgcgaag
     1801 ttatcgtaaa agatacccgg ctgataccga acgtgagcca gatgttcatc gggctgcttg
     1861 aaacggaaga gagggcggtg gcgcccaacg attcgagcag aggagggact agtgctccac
     1921 gttggcctcg acgtgggctc ggtcagcgtc aagctggcgg ccctcggcgc tcgcgaagac
     1981 tccgccgttc tttccctgct ggctgaaacc agctccgcct tcttcgcgcc caggttgcct
     2041 gcggcaaacg gatgggcgga gcgtcctgtc gtgtgttccg cgtatcggcg catccaggga
     2101 aatcccctgc aggcggcatc ggaactgttg cgggagttct acgcaatcgt tcctcaggaa
     2161 tcgatctcag gtattcgctt caccgggtcg ggtggacgga cggtggcggc tgcgttcgaa
     2221 gccgagtttg agaacgactt tcgcacgctc gttcgatgca ttcgagcgct ttatccgcaa
     2281 gtccgcactg tgttcgaaat gggcggagag gcctcgaaat atatccggtt gagcgatgtg
     2341 ggcagctcaa gcaacctcgg agtcctggat taccagtcga gcaccgagtg cgcagcggga
     2401 acgggatctt tcatcgatca gcaggcgtcg cgcctgcgct acgacgtaga ggatgtcggc
     2461 gaggcggcgt gccatgcgag ttgcgctgcc cgcgtggcag gccgatgctc ggtgttcgcc
     2521 aagacagaca tgatccacgc ccagcagaag ggatacacgg cagaccagat cctgcgcgga
     2581 ctgtgcgacg cggtggccag aaacttcaaa tccagcatcg tcaagggacg acaaatcgcg
     2641 acgccggtgg cattcgttgg cggcgtggcg ctgaatgccg gtgtgtacga ggcgctgcgg
     2701 gaggcattcg ggctcaggga atcagattgc ctggtgcccg atctgaattg ctggatgccc
     2761 gccctgggcg caagcatctg tggtgtcgaa gcaggcgcga agcgcaccac ggttgcgctc
     2821 caccaagttc ccccgcaaat tgcagcggga gaggaaatcc ctaccaccgg gcctctttcg
     2881 ctcaacaacg taactctgct gaaccgagct ccggcggtcg cgctgccgca gggagcgggc
     2941 tgcgtcgatg cttacctcgg aattgacgtg gggtcggtga gcacgaatct ggtggtactg
     3001 g
//
LOCUS       DASPUL010000511         3490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1595736, whole genome shotgun sequence.
ACCESSION   DASPUL010000511 DASPUL010000000
VERSION     DASPUL010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595736"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..1402)
                     /locus_tag="VEJ00_12055"
     CDS             complement(344..1402)
                     /locus_tag="VEJ00_12055"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HXX01939.1"
                     /translation="MNQITTVGVDLAKDLIVACAADAAGRTQFYKRLGFHGFAAWAAN
                     LPPCTIAMEACSSAHYWARTLSALGHNVKLLAPDLVKPFRKSQAAKNDRNDAQAILIT
                     ALQPDMRFVSMKSLEQQSILAVHRMREGWKSERTALINRVRGLLAEFGFWLGRSPQTL
                     ARRLPQLMQEEQLPARVRALLAQAHGHLLQLEALMQQCELDIQAHACHSEAAQRLSAL
                     NGIGPITASAIVATVGNAADFRNGRQMAAWCGLVPKQSSSGGKERLGKITKRGDTYLR
                     GLLTQGARSTLNAALARPLEKRSRLEHWIVALQQRVGYHKSLVAIANKHARICWALLA
                     RAERYDPHAASRHMRAAA"
     gene            1706..3133
                     /locus_tag="VEJ00_12060"
     CDS             1706..3133
                     /locus_tag="VEJ00_12060"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HXX01940.1"
                     /translation="MADQTSSPGTSQEKTATTANKLTNVSVAQQSSITGLSATEAQAI
                     AKEAYIYGYPMVDGYRIQYAYFVDVGNKEYKAPWNQICNISRVYTPEDKEVTTPNSDT
                     PYSMLGLDLRAEPIVLTVPAIEKDRYYSVQFIDLYTFNFGYAGSRSTGNEAGNFLLAG
                     PKWKGEKPEGIKAVIPCETELALVAYRTQLFGPDDLDNVKKVQAGYKVQPLSGFLGKP
                     TLAAPAIDFFKPIPEDQQRTSPEFFNVLNFILKFCPTHPSETELRARFAKLGIGPDGA
                     FDAAKLSPEISKAIGAGMADAWQAFADLKKKVDAGQVTSGDAFGTREHLKNNYLYRMA
                     AGVMGIYGNSQQEAMYPVLSVDSEGQKLDAAQNRYTVRLAPDQMPPVNAFWSLTMYEL
                     PGSRLTANPINRYLINSPMLPSLKRDADGGLTVYIQNDSPGKDKESNWLPAPKGPFIT
                     IMRLYWPKDEALNGKWKPPAVARAR"
     gene            complement(3303..>3490)
                     /locus_tag="VEJ00_12065"
     CDS             complement(3303..>3490)
                     /locus_tag="VEJ00_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HXX01941.1"
                     /translation="LRRSTVDHPFATIKAWMGATHFLTKTLPKVRTEMSLHVLAYNMK
                     RAFKILGTRTLIQAIGA"
BASE COUNT          696 a         1024 c         1075 g          695 t
ORIGIN      
        1 atctctacgg agttccccgt caagttcggc gcgatgttga tcgagattct tgatctgggc
       61 ggagcacgtg atcgcgcttg gggctgcttg gcagataggc gcagagagtc tgactgcatc
      121 tctctacacg gccttgtggg atgagtgtga gtggtcatcc gggccaaggt ataagtcgcg
      181 cacggtgtcc tcactcgttg tgcggcattg acccgcgagg atgatcggtc cactcgggtt
      241 ggttcacgcc ggattgttag ccaggttttc accgtgctcg gtctgacctc gttgcctatc
      301 tggtgtggca gcgcaagggg tgggttcgca aggattggta ctctcaggcc gctgcgcgca
      361 tgtggcgcga tgcggcgtgc gggtcgtagc gctctgcgcg cgccaggagt gcccagcaga
      421 tcctggcgtg cttgttggcg atagcgacga ggcttttgtg atagccgacg cgctgttgca
      481 gagccacgat ccaatgctcc agcctcgaac gcttctcgag aggtcgggcg agtgcggcat
      541 taagcgtgga gcgcgcccct tgggtgagca ggccgcgcag atacgtgtcg ccgcgcttgg
      601 tgatcttgcc gagccgctct ttgccgccgg agctggattg cttgggtact aggccgcacc
      661 aggcggccat ctggcggcca ttgcggaagt ccgcggcgtt gccgacagtg gcgacaatgg
      721 cgctggccgt gattgggccg atgccgttga gagcgctaag ccgctgcgcc gcttcgctgt
      781 ggcacgcatg cgcttggata tcgagttcgc attgctgcat cagcgcttca agttgcaaca
      841 ggtgcccgtg agcctgggcg agcagtgcac gcacgcgtgc gggaagctgt tcctcctgca
      901 tcaattgcgg aagccttcga gccagagttt gcggcgagcg cccgagccag aagccaaatt
      961 ccgccagcag cccccgcacc cggttgatca gtgccgtgcg ctcgctcttc cacccctcgc
     1021 gcatgcgatg caccgccagg attgactgct gctcgaggct cttcatcgac acaaaacgca
     1081 tgtccggttg cagcgcagtg atcaggattg cctgcgcatc attgcgatcg ttcttggccg
     1141 cctggctctt gcgaaaaggc tttaccagat ccggcgccag caacttgacg ttatgcccga
     1201 gtgcactgag cgtgcgcgcc cagtaatgcg ccgagctgca agcctccatc gcgatcgtgc
     1261 acggcggcag gttcgccgcc catgccgcaa aaccgtgaaa acccagccgc ttgtagaact
     1321 gcgtgcggcc tgccgcatcg gccgcgcacg cgacgatcag atcctttgcg aggtccacac
     1381 cgacggtagt aatctggttc atgagttccc cttttctgat gcagattgaa tgtgacaact
     1441 caatcttggc actctgatgc cgtacgactc acgtcgatca gggaagggga actcccttat
     1501 attcgttcgg gctatacgcc ggccgagctt gagctaatga tcggatggcg tgagcctatg
     1561 gtccgatacc cacctcgcga gctgtagctg acactcgtca tcgcgtaggc ttccccgtcg
     1621 aggaactgaa aaattgcacc aatgattgat ttggtcggta cccgccggtc tcgggtcggc
     1681 ccacagcaat cgagggagta ttcctatggc tgaccagacg tcttcaccag gaacttctca
     1741 ggaaaagaca gccacaactg caaataagct cacgaatgtg agtgtggccc agcagtcctc
     1801 cataacagga ctgtccgcga cggaagcaca ggcgatcgca aaggaagcct acatctacgg
     1861 ctacccaatg gtggacggct accgcatcca atatgcgtac ttcgtagacg tcggaaacaa
     1921 ggagtacaag gcaccgtgga accagatctg caacatctcc cgggtttaca ccccggaaga
     1981 caaggaggtg acgacgccta actcggacac gccatattca atgctgggcc tggacctgcg
     2041 tgccgagccg atcgtgctca ccgtgccggc gatcgagaaa gaccgctact actcggtcca
     2101 gttcatcgat ctctacacgt tcaacttcgg ctacgccggc agccgctcaa ctggcaacga
     2161 ggcggggaac ttcctgctcg ccgggccgaa gtggaaaggc gagaagcccg agggaatcaa
     2221 ggcggtgatc ccctgcgaga cggagttagc gctcgtggcc taccgcacgc aactgtttgg
     2281 gccggacgac ctcgacaacg tgaagaaggt ccaagcgggt tacaaggtgc agccgctgtc
     2341 ggggtttttg ggcaagccca cgctcgccgc gccggccatc gatttcttca agccgatccc
     2401 ggaggatcag cagcgcacgt caccggagtt cttcaacgtc ctgaacttca tcctgaagtt
     2461 ctgccccact catccctctg agacggagct gcgggcgcgc ttcgccaagc tcgggatcgg
     2521 cccggacggc gcgttcgacg ccgcgaagct atcacctgag atcagtaaag ccatcggggc
     2581 gggcatggcg gacgcatggc aagcattcgc cgacctgaag aaaaaggttg atgccgggca
     2641 agtgacatcc ggtgacgcct tcggcacgcg ggagcatttg aaaaacaact acctgtaccg
     2701 catggcggcg ggtgtcatgg gcatctacgg caactcccag caggaagcga tgtacccggt
     2761 gctttccgtg gattccgaag gacagaaact cgacgccgcc cagaaccgtt acaccgtacg
     2821 ccttgcgccg gatcaaatgc cacccgtcaa tgcattctgg tcgctgacga tgtatgaact
     2881 gccggggagc cgtttgacgg cgaatccgat caaccgctac ctgatcaact cgccgatgct
     2941 gccgagcttg aagcgcgacg cggatggcgg cttgaccgtt tacatccaga acgattcgcc
     3001 ggggaaagac aaggagtcca actggctacc cgcgccgaag ggtccgttca tcacgatcat
     3061 gcgcctttac tggccgaagg acgaggcatt gaacggaaaa tggaagcctc cggcggtggc
     3121 gcgggcccgg taaccggcta gccgtccggg ttgccggtcg aacggtcaac gttgggatcg
     3181 aacgtccggt tacggggaag ctatacggtc aagctgtatg cccgcttctg gccgaggccg
     3241 tgtgaaaacg cgcgcttttg tgcaagattt tgctaaataa aaggaagagt gaagtcatcg
     3301 ggctaggccc cgatcgcttg gataagagtc cttgtcccga ggatcttgaa cgcccttttc
     3361 atgttgtacg cgagaacgtg caggctcatc tcggttctga ccttggggag cgttttcgtc
     3421 aggaagtgcg tagcgcccat ccacgctttg atcgtcgcga agggatgatc gacggtggag
     3481 cggcgcagct
//
LOCUS       DASPUL010000512         3765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_94389, whole genome shotgun sequence.
ACCESSION   DASPUL010000512 DASPUL010000000
VERSION     DASPUL010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94389"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1652)
                     /locus_tag="VEJ00_12070"
     CDS             complement(57..1652)
                     /locus_tag="VEJ00_12070"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXX01942.1"
                     /translation="MAISRREFLRNGALATGALITQHGGKTRPTVQARPSLNPNSLTP
                     FVDPLPIPSAAHSIGVRPSPTNPSIKIPCYRVALQSFETSVHRDMKPTRMWGFGGAFP
                     GPTFDLRSSEEVLVEWANELPKQHFLPIDHTLHGAEKDKPEVRAVAHLHGARVPPESD
                     GYPEDWITPGKSAVYHYPNQQDAAMLWYHDHAMGINRLNIFAGLAGAYILRDKQEDAL
                     HLPTGKYEIPLVICDRMFDKDSQLYYPVSDKPESPWTPEFFGDANLLNGKLFPFLEVE
                     PRKYRFRVLNAANGRFYHLSLSNKLPFHQIGTDQGLLSAPVTAQSIMIAPGERLDLVI
                     DFSQSAGTSIVLNDDFVPLMQFRVSGTNVKDDSILPATLRRIEKLPESAAVRTRLLSL
                     DEEDDLVQNPVRMLLNGKSWHDPVTENPVLNSTEIWSFINPTDDSHPIHLHLVKFQIL
                     DRRKIENFAYISQQELRFTGPPVPPDPDEAGWKDTVRAHPGMMTRIIARFEGYPGRYV
                     WHCHILEHEDNEMMRPYDVLPSS"
     gene            complement(1797..3428)
                     /locus_tag="VEJ00_12075"
     CDS             complement(1797..3428)
                     /locus_tag="VEJ00_12075"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXX01943.1"
                     /translation="MRRLFAILLAAVLLDPGLIYAGPTPGGYGSTATPIKHVVIIFGE
                     NVSFDHYFGTYPSALNLKGEPTFFARPDTPSVNGYSPALLTANPNLNPANGAGATNPF
                     RLSPSQASTADQDHNYTPEQQALDGGIVDLFPKFTGTGGTATGGKGSPAVFNTTGLTM
                     GYYDGNTLTALWNYAQHFAMNDNSYDTNFGPSTPGALNLISGQTNGAINNVNGTGALI
                     NDGGTGLTDIGDADPNGDVCSTSTGERFNMSGKNIGDLLNQAGVTWGFFEGGFDLTVT
                     NANGTTGCKRSTTSAVTNVTKADYIPHHQPFQYYASTANPTHARPASIAEIGNNGPAN
                     HQYDTHDFFDAVSKGNFPAVSFLKAAGFQDAHAGYSDPLDEQTFVVNTINFLMKTPEW
                     RNTVVIISYDDSDGWSDHQMGPIVNQSQTAADALNAPGLCGQSTANILPGVDIAAAQG
                     RCAYGPRLPFLVISPYARKNFVDHTTTDQSSTIRFIEDNWLGGERIGNGSFDAIAGPI
                     NNMFDFDHDRDNDQDGILILNPSNGQVVGQGFFGW"
BASE COUNT          763 a          992 c         1197 g          813 t
ORIGIN      
        1 agactaatgc cattatccat aagtgagcag gtagtttcca gtaaaactca gggaacctac
       61 gaggacggaa gtacatcgta gggccgcatc atctcattgt cttcatgttc caggatatgg
      121 cagtgccaga cataccgccc cggatagcct tcgaatcgcg caatgatcct tgtcatcatt
      181 cccgggtgcg cgcgcaccgt gtccttccag ccagcttcgt ccggatcggg cggaaccggc
      241 gggcccgtga atcgcaactc ctgctgactg atatacgcaa agttttcgat cttccgccgg
      301 tccaggatct ggaatttcac cagatgcagg tggatgggat gggagtcatc ggtcggattt
      361 atgaaagacc agatttccgt agagttgagg accggatttt ccgtgaccgg atcgtgccag
      421 ctcttgccgt tcagcaacat cctgacggga ttctgcacca ggtcatcctc ttcatcgagc
      481 gaaaggaggc gcgttcgcac ggccgccgac tccgggagct tctcgatgcg gcgaagcgtg
      541 gctggcagga tgctatcgtc tttcacattg gttcccgaaa cgcgaaactg catcagcggg
      601 acgaaatcat cgttcaagac aatgctggtt cccgcgctct ggctgaaatc gatgaccagg
      661 tccagccgct cgcctggggc gatcatgatg gactgcgcag tgaccggcgc cgagagcagt
      721 ccctggtccg tcccaatctg atgaaacggc agcttgttgg agagggacag gtggtagaag
      781 cggccgttgg cggcgttgag cacgcggaag cgatacttgc gcggctccac ttccagaaac
      841 gggaacaatt tcccgttcaa cagattcgca tcgccaaaaa actccggggt ccagggcgat
      901 tccggcttgt ccgaaacggg atagtacagc tggctgtcct tatcgaacat gcggtcgcaa
      961 attacgagag ggatttcgta tttgccggtg ggcaagtgca gagcgtcttc ctgcttgtcc
     1021 cgaagaatgt aggcacccgc gagtccggcg aagatattca ggcggttaat gcccatcgca
     1081 tgatcgtggt accagagcat ggctgcgtcc tgctgattcg ggtagtgata cacggcggac
     1141 tttcccggag tgatccagtc ctccggatag ccgtcgcttt ccggcggcac cctggcgccg
     1201 tgcaagtgcg ccacggcgcg gacttccggc ttgtctttct cggcaccgtg aagagtgtga
     1261 tcgatcggca agaagtgctg cttagggagt tcgttggccc attccaccag gacttcctcg
     1321 cttgagcgca aatcgaatgt cgggccggga aatgcgccgc cgaatcccca catccgcgtc
     1381 ggcttcatgt ctcgatgcac gctggtctca aaggactgca gcgccacgcg atagcagggg
     1441 attttgatgc tgggatttgt ggggcttggc cgcacgccta tgctgtgggc ggcggacgga
     1501 atgggcagag ggtccacaaa cggagtcagg gaattgggat tcagggacgg gcgcgcctga
     1561 accgttggcc gagttttccc tccatgttgc gtgatcagcg cgcccgtagc cagcgcgccg
     1621 ttgcgcagga attcgcgcct ggagattgcc atgccttagc ctcttttccg aaaaagaaag
     1681 attgtacgcg caaaaaagca gcgggctcac gaacgagatt cgcggcccgc tgcttcacgc
     1741 actcagttga gagggaaaag ctcctcccac cctgcctggt tcatcgtaat gggcaattac
     1801 cagccaaaga agccctgacc cacgacttgc ccgttgctcg gattgaggat gaggattcca
     1861 tcctggtcgt tgtcgcgatc gtgatcgaag tcgaacatgt tgttgatcgg accggcaatg
     1921 gcgtcaaatg agccgttacc gatgcgctcg ccgcccagcc agttgtcctc gatgaaacgg
     1981 atggtcgagc tctgatcggt ggtggtgtgg tcaacgaagt tcttcctggc gtagggagag
     2041 atcaccagga agggcaaacg cggaccatag gcgcaacggc cttgcgcggc ggcaatgtcg
     2101 acgccaggca agatgttcgc agtgctctgt ccgcaaaggc cgggcgcgtt caaagcgtcg
     2161 gcagccgtct gggactggtt cacgatgggg cccatctggt ggtcagacca gccgtcggaa
     2221 tcgtcatagc tgataatcac gacggtgttg cgccattccg gcgtcttcat caggaagttg
     2281 atggtgttga ccacgaaggt ctgctcgtcc agagggtcgg agtagccggc atgggcatcc
     2341 tggaagccag ccgccttcag gaagctgacg gcggggaagt tccccttgct gacggcgtcg
     2401 aagaagtcgt gcgtgtcgta ctggtggttt gccggaccgt tattgccgat ctccgcgatg
     2461 gaagccgggc gcgcgtgcgt cgggtttgcc gtggaagcat aatactggaa cggctggtgg
     2521 tgcgggatgt agtcggcttt cgtaacattg gtgaccgccg aggtcgtgga gcgcttgcag
     2581 ccggtcgtgc cattggcatt cgtgaccgtc agatcgaagc cgccctcgaa gaagccccag
     2641 gtgactcccg cctggttcag aagatcgccg atgttcttgc cgctcatatt gaatctctcg
     2701 cccgtggacg tggagcatac atcgccgttc gggtcggcat cgccgatgtc ggtcagaccg
     2761 gtgccgccgt cgttgatcag ggcgccggtg ccgttcacat tgttgatcgc gccgttggtc
     2821 tggccggaga tcaggttgag cgctcccggc gtggaggggc caaagttcgt gtcatacgag
     2881 ttgtcgttca tcgcgaagtg ctgcgcatag ttccacagcg cggtcagggt gtttccgtcg
     2941 tagtagccca tcgtgagccc ggtggtgttg aagaccgccg gtgaaccctt gccacccgtg
     3001 gccgtgccgc ccgtgccggt gaacttgggg aaaagatcga cgattccgcc atcaagcgcc
     3061 tgctgttcgg gcgtgtagtt gtgatcctga tccgccgtgg aggcctgcga ggggctcaga
     3121 cggaacgggt tcgtggcgcc ggctccgttc gccggattca agttgggatt cgcggtcagg
     3181 agagctgggc tgtagccgtt gaccgatggg gtgtccggac gcgcgaagaa agttggctcc
     3241 cctttcaggt tcagagccga gggatatgtg ccaaagtagt gatcgaaaga tacgttctct
     3301 ccaaaaatga taacgacatg cttgatgggt gttgcggtgg aaccataacc cccaggtgtt
     3361 ggaccggcgt aaatcagtcc cggatcgagc agaactgcag caagcaagat ggcaaacaat
     3421 cttcgcatat ttgagagtgg cctccattgg gttctgacgc cgacctggtg gcagtcatta
     3481 aaacaaaacc attggagagt atcggcggaa ggttacggtt cgatgacaaa gtgacgcggc
     3541 gtaggtaaaa gaaatgtcac ttttgaaagt ttgtgaagat ggtccgtgaa atttgctgcg
     3601 cgaaagtatg cgagtttgaa gaatcgcaac gcgatggggc acggattaga gattttctgg
     3661 ggacctgggc taaactaggt gaggatgaaa tagtgttcct tgtttgcccg tgcgggagtg
     3721 gcggaactgg cagacgcgca agacttagga tcttgtgggg taaca
//
LOCUS       DASPUL010000513         2760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2017667, whole genome shotgun sequence.
ACCESSION   DASPUL010000513 DASPUL010000000
VERSION     DASPUL010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2017667"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1755)
                     /locus_tag="VEJ00_12080"
     CDS             complement(<1..1755)
                     /locus_tag="VEJ00_12080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HXX01944.1"
                     /translation="MPPTTPSRSAGGAATLHQFFAPGGVLSRTHPAYEFRRGQLQMAQ
                     AVEQAMEEKRHLIVEAGTGTGKTLAYLLPVIRSGKRVIISTGTKNLQEQLFYKDVPFL
                     EQALYAEREETASAVQHLGRLSVCYMKGRNNYLCRKKLYDLTDQPVLSGLDEIEQYRA
                     IAAWEKTTQTGDRSELAELPEASLLWHKLDGRADACTGQNCSQWDRCFITEMRRRALE
                     SDIIVVNHHLFFADLAIKLQAEAAPDAGILPEAATVIFDEAHELEDVAGNYFGISVSN
                     LRVEELARDVENSLQRNRMLSTSLSGALASLRERSQFFFSLLPPGEGRFAFETRREFL
                     EENGDEFLALNQALTRLAGELEGLPQKPEEIFNFVRRAQEIQLQLGFALESDDRNTVF
                     WIERRGTRGPGRGRQNREGHGFSPAGSSASNKGAVAPGGVPQGRQNIFLQATPIDVAP
                     ILRECLWSKLECAVLTSATLAVGGGFEYIRQRLGLEHPRECVLPSHFDYAKQALLYVP
                     PDLPDPRTPQFAAKAAGCIRRLLEITRGRAFVLFTSYAQMNEIHQRLLGEVEFPLLRQ
                     GDAPKSALLEEFRLTPSAV"
     gene            1823..2605
                     /locus_tag="VEJ00_12085"
     CDS             1823..2605
                     /locus_tag="VEJ00_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HXX01945.1"
                     /translation="MPAPAAPAPSVRLWRLDCGTVQVNDLNVFSDTYAYVGQSKRLTA
                     SCYLIQHDDTYMLWDTGLPEATMGASLSASGAMAASLSRTIKDQLSELKVSPASISLI
                     GVSHYHWDHTGQAASFPQAKLLMGKGDVEALRQSGGERAKPLEHWLTGGGALEEITGD
                     KDVFGDGSVVMLDLPGHTPGHHGLLVKLAKTGYVLLSGDVAHFRENLESDGIPAFNED
                     RTKSLASLDRFKRISKNLKAVTIVQHEPEDVAKLPPFPKSAD"
     gene            2618..>2760
                     /locus_tag="VEJ00_12090"
     CDS             2618..>2760
                     /locus_tag="VEJ00_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HXX01946.1"
                     /translation="MRISPNPLVAIVGRPNVGKSTLFNRIVGSRRAIVGDEPGITRDR
                     LYG"
BASE COUNT          564 a          837 c          810 g          549 t
ORIGIN      
        1 cacggcgctc ggcgttaagc gaaattcctc gagcagggcg ctttttggcg cgtctccctg
       61 gcgcaagagg ggaaactcga cctcgccgag caaccgctga tggatttcgt tcatctgcgc
      121 gtaactggtg aagagcacaa aggcccgccc gcgcgtgatt tcgagcaacc ggcgaatgca
      181 tccagcggct ttggcggcga attgcggagt gcggggatca ggcagatcgg gaggcacgta
      241 aagcaacgcc tgcttcgcgt agtcaaagtg ggatggcaat acgcactccc gcggatgttc
      301 gaggccaagc cgctgccgga tgtactcaaa acctccgcca accgccagtg tggcagaggt
      361 cagcacagca cactcaagtt tcgaccacaa acactcgcgg aggatcgggg cgacgtcgat
      421 gggggtagcc tgaagaaaaa tgttctggcg gccttgaggc acacccccag gggctacagc
      481 tcccttattg cttgcactgc ttccggcagg gctaaagccg tgcccttccc gattctgcct
      541 tccgcggcca ggtccacgtg ttcctcttcg ttcgatccag aacacagtgt tgcggtcgtc
      601 cgactccaat gcaaagccga gctgaagttg aatttcctgt gcccgccgca cgaagttgaa
      661 tatttcttct ggcttctggg gcaggccctc caattcgccc gccaggcgag tcaaagcctg
      721 gttgagagcc agaaactcgt ccccattttc ttcgagaaat tcgcggcgag tctcgaaagc
      781 gaagcggcct tcgcccgggg gcagcagaga aaagaagaac tgggagcgct cccgcaggct
      841 tgccagtgct cccgacaggg atgtggaaag catccggttg cgctgcagag agttctcgac
      901 gtctcgcgcc aactcctcga cacgcagatt actcaccgaa atgccgaagt agttgccggc
      961 aacgtcttcc agttcgtggg cttcatcgaa aatgactgtg gcggcctccg gcaggatacc
     1021 agcgtccggc gcggcttctg cttgcagctt gatggcgaga tcggcgaaga acaggtgatg
     1081 attgacgaca atgatgtcgc tctccaaggc ccgacgccgc atttcggtga tgaagcagcg
     1141 atcccactgg ctgcaattct ggccggtgca ggcatcggcc cggccatcga gcttgtgcca
     1201 aagcaggctt gcttcgggca gttccgccag ttccgagcga tcgccggtct gcgtcgtttt
     1261 ctcccaggcg gcgatcgctc ggtactgctc aatctcatcc aaaccgctga gcaccggctg
     1321 atcggtaaga tcgtagagtt tcttgcggca cagatagttg ttgcggcctt tcatatagca
     1381 cacggaaagc cgcccgaggt gctggaccgc cgaggccgtc tcttcgcgct ctgcataaag
     1441 agcttgctcg aggaaaggaa cgtccttgta gaaaagttgc tcctgcagat tcttggtacc
     1501 ggtggagatg atgacgcgct tgcccgagcg aatcaccggc agaagataag ccagcgtttt
     1561 ccccgtacct gtacccgcct cgacgatcaa gtggcgcttc tcctccatcg cctgctcgac
     1621 cgcttgcgcc atctgcagtt gtccgcggcg gaattcatac gcgggatgcg tgcgcgaaag
     1681 cactccaccc ggcgcaaaaa actgatgcag cgtcgcagcg ccacctgcag aacgggatgg
     1741 agtcgtgggg ggcacgggaa tctctataat agtgcctatg ctccgctcaa ttttctccct
     1801 atcgctcttg gtcgcacttt ctatgcccgc cccagcagcc cctgcgccat ccgtgcgctt
     1861 gtggaggctt gattgcggca cggttcaagt caacgacctt aacgtgtttt ccgacaccta
     1921 tgcttatgtc gggcaatcga aacgcctaac cgcgagctgc tacctcatcc agcatgacga
     1981 cacatacatg ttgtgggaca cggggctccc ggaagccacc atgggggcat cactgagcgc
     2041 gtccggcgca atggccgcca gtctgtcccg cacgatcaag gatcagctat cggagctcaa
     2101 ggtttctccc gcatcgatct ccttgattgg tgtcagccac tatcactggg atcataccgg
     2161 acaggctgcg agtttccctc aagcgaagct gctcatggga aaaggggacg tcgaggcgct
     2221 gcggcagtcc ggcggggaac gcgccaagcc actggaacac tggctaaccg gcggcggcgc
     2281 tctggaagag atcaccggcg acaaggacgt ctttggggac ggcagcgtag tcatgctcga
     2341 ccttccaggg cacacgccgg ggcaccacgg tctgctcgtg aaactggcga agaccggcta
     2401 cgtgctgctg tctggggacg tcgcccactt ccgcgagaat ctcgaaagcg atggcattcc
     2461 ggccttcaat gaagatcgca caaaatcgct cgcgtcactt gatcgattca agaggatttc
     2521 gaagaacctg aaggctgtca caatcgtcca gcatgagccg gaagacgtcg ctaaactgcc
     2581 gccatttccc aaatctgccg actaaccggc ccgtagcttg cgcatcagtc ccaatccgct
     2641 cgtcgccatc gtgggccggc ccaacgtggg caagtccacg ttgttcaatc gcatcgtggg
     2701 ctcgcggcgt gccatcgtgg gtgacgaacc ggggatcacg cgcgatcgac tctacggtga
//
LOCUS       DASPUL010000514         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1464085, whole genome shotgun sequence.
ACCESSION   DASPUL010000514 DASPUL010000000
VERSION     DASPUL010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1464085"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VEJ00_12095"
     CDS             complement(<1..988)
                     /locus_tag="VEJ00_12095"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXX01947.1"
                     /translation="MPKTSQLAAVPSQAAESTRDFDVLIIGAGLSGIGAGYHLQDKCP
                     RKSFAILEGRSAIGGTWDLFRYPGVRSDSDMHTLGYRFRPWSEAKAIADGPSILSYIR
                     ETAGEYGIDRKIRFNRRVVRASWSSPDARWTIEATGPDGPVEFTCNFLYMCSGYYEYE
                     SGYMPGWPGMDRFTGRTVHPQQWPEDLDYTGKRVVVIGSGATAVTLVPAMTDKAAHVT
                     MVQRSPTYVVTRPSEDAIANWLHRRLPGRLGHALSRWKNILLQMYFYGVARLKPDATK
                     KSILKFAQLQLGPDYDVAKHFNPRYNPWDQRLCLVPDGDLFAAIRAGKASVVT"
     gene            complement(1121..1726)
                     /locus_tag="VEJ00_12100"
     CDS             complement(1121..1726)
                     /locus_tag="VEJ00_12100"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HXX01948.1"
                     /translation="MHSSRTAHLFERSAHPPNHYLIAHSDGGARGNPGPAGYGVVIKD
                     NSGRKVAALSQYLGHQTNNFAEYQGLIAALEYALEHGPRALQLISDSELLVRQIKGIY
                     KVKNPTLKDLHARAKELIAQLDWFSIGHALREHNQEADRLANEAMDKGMGREARALPP
                     AESSAPSAAPQEFEGIVCDGAIKLLNGTLPEGTRVQVRTKR"
     gene            complement(1856..2611)
                     /locus_tag="VEJ00_12105"
     CDS             complement(1856..2611)
                     /locus_tag="VEJ00_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXX01949.1"
                     /translation="MRILLISDVHANLEALEACLADAPAYDLVVNLGDIVGYGASPNE
                     VTDRSRDLGKIFVRGNHDKAATGVMDLDDFNPMAAAAAIWTRNELTSENLDWLRTLPH
                     GPVTMPDFPQVHLVHGSPNDEDEYVVSLGDALAPLIALPVPLTFFGHTHLQGGFFANG
                     TSADGFRPEYRTVGRSESVPLQLKSTSRYLINPGSVGQPRDGDWRAAFALFDTDAQVI
                     HFHRTPYNLKAAQDRIFAANLPSRLATRLAAGR"
     gene            2794..>3040
                     /locus_tag="VEJ00_12110"
     CDS             2794..>3040
                     /locus_tag="VEJ00_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HXX01950.1"
                     /translation="MADLTANCIFCRILRGELPARKVHEDEHVFAIEDIHPQAPTHVL
                     IIPKRHFAGLKEAEAADAEVIGRCHLVAAQIARARNIE"
BASE COUNT          551 a          830 c         1075 g          584 t
ORIGIN      
        1 cggtcacgac cgaggctttg ccggcgcgga tcgcggcgaa cagatcgcca tcgggaacga
       61 ggcagaggcg ctggtcccag gggttgtagc gggggttgaa gtgcttggcg acgtcgtagt
      121 cgggaccgag ttggagctgg gcgaacttga ggatggattt cttggtggcg tcgggtttca
      181 ggcgcgcgac accgtagaag tacatctgca gaagaatgtt cttccagcgc gaaagcgcgt
      241 gcccgagccg gcccggaaga cggcggtgca gccagttggc aatggcgtct tcggagggcc
      301 gggtgacgac gtacgtgggc gagcgctgca ccatggtcac atgcgcagct ttgtcagtca
      361 tcgccggaac cagcgttaca gcggtggcgc cgctgccgat gaccacgacc cgcttgcccg
      421 tgtaatcgag atcctcgggc cattgctgcg gatgcacggt gcggccggtg aagcgatcca
      481 tgccgggcca gcccggcatg tatccggact cgtattcgta atagccgctg cacatgtaga
      541 ggaagttgca ggtgaactcg accggtccat cggggccggt ggcttcaatg gtccagcggg
      601 catcgggcga agaccaggag gcgcgcacga cgcggcggtt gaagcgaatc ttgcggtcga
      661 tgccatactc cccggcggtt tcgcggatgt agctaaggat cgagggaccg tcagcgatgg
      721 ccttggcttc gctccagggg cggaatcggt aacccagggt gtgcatgtcg gagtcggagc
      781 ggacaccggg atagcggaag agatcccagg tgccgccgat ggcgctgcgg ccttcgagga
      841 tggcgaaact cttgcgcggg catttgtcct gcaggtgata gccggcgcca atgccggaca
      901 gtccagcgcc gatgatcagg acgtcgaagt cccgtgtaga ttcggcggcc tgcgacggaa
      961 cggccgccag ttgcgatgtt ttcggcataa gagcggttca gggcaggggg aagttaaccc
     1021 gagaggactt gcacggccct ctttcgtcta cccacattgg cgtgcggcgg ctggctttgt
     1081 caaggggagg cgttggtctc tgtgccgctt taaggccggg ctatctcttc gtccgcacct
     1141 gcacgcgcgt gccctcgggc agagttccat ttaacaactt gatcgcgccg tcgcacacga
     1201 tgccttcgaa ttcctgtggg gctgccgatg gagcggagct ttccgcgggc gggagcgccc
     1261 gcgcctcacg gcccatgcct ttgtccatgg cttcgttggc caggcggtct gcttcctgat
     1321 tgtgctcccg cagcgcgtga ccgatggaga accagtcgag ctgcgcaatc aattccttgg
     1381 cgcgggcgtg gagatccttc agcgtggggt tcttgacctt gtagatgcct ttgatctgcc
     1441 gcacaagcag ctcggaatcg ctgatgagct gcagcgctct tggaccgtgt tcgagcgcgt
     1501 attcgagggc ggcgatcaat ccctgatatt cggcgaagtt gttggtctgg tggccgaggt
     1561 attggctgag ggcggcgact tttcgtccgg agttgtcttt gatgacgacg ccgtagccgg
     1621 cgggcccggg gttgccgcgc gcgccgccgt cgctgtgcgc gatgaggtaa tggttggggg
     1681 gatgagccga gcgttcgaag agatgtgcgg tgcgcgagga atgcatcggt tcgagtttac
     1741 attggtttat gagatgcgta gatgatcgac gcgaacggaa aaggaaacca caggggctga
     1801 agccccatca gactcgacgc cctaacgcgg ccctgaaggg ccgctcttcc actttttagc
     1861 ggcccgcggc taaccgagtg gccaggcgtg atggcaagtt ggcggcgaag atgcggtcct
     1921 gggccgcttt caggttgtag ggggtgcggt ggaagtggat gacctgggcg tcggtgtcga
     1981 acagggcgaa agcggcgcgc cagtcgccgt cgcggggctg gcccacggag ccggggttga
     2041 tcaggtagcg cgacgtggac ttgagctgca aggggaccga ctccgatcgt ccgacggtgc
     2101 ggtactcggg acggaatccg tcagcagacg tgccgtttgc gaaaaatcct ccttgcaggt
     2161 gcgtgtggcc gaagaaagtc aggggcacgg gaagagcgat cagcggcgcg agggcgtcgc
     2221 cgagagaaac gacgtactcg tcttcgtcgt tgggagagcc gtgaaccaga tgcacctggg
     2281 gaaaatcggg catggtgacg ggaccatgcg gcagggtgcg gagccagtcg aggttttccg
     2341 aagtcagttc gttccgggtc cagatggcgg cggcagcggc catgggattg aagtcgtcga
     2401 ggtccatgac tccggtggcg gccttgtcgt ggttgccgcg cacgaaaatc tttcccaaat
     2461 cgcgggagcg gtcagtgacc tcgttggggc tggcgccgta gccaacgatg tcgccgaggt
     2521 tgacgacaag atcgtaggcg ggagcgtcag cgagacaggc ttccagcgct tcgagattgg
     2581 cgtgaacgtc gctgatcagc aatatgcgca aggatcaccg gaccctcaac cgtacaagca
     2641 accgggcaag ctgccgatta tagaacggaa actgcagtta gaggagagca gcgcggcgga
     2701 gttcgccgct ccgaacacaa ctccgtactg ctgacagatt cgcggctgcg gtgatatcgt
     2761 tgccttcgcc agttgcgcct gccactgcct cgtatggccg atctcactgc caattgcatc
     2821 ttctgccgca tcctgcgcgg cgagcttcct gcgaggaaag tacacgagga cgaacacgtt
     2881 tttgctattg aggacatcca tccgcaggcg ccgacgcatg tgctgatcat tcccaagagg
     2941 cattttgcgg ggctgaagga ggccgaggcg gcggatgccg aggtcatcgg acgctgccac
     3001 ctggtggcgg cacagattgc gcgcgctcgc aatatcgaac
//
LOCUS       DASPUL010000515         3426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1192452, whole genome shotgun sequence.
ACCESSION   DASPUL010000515 DASPUL010000000
VERSION     DASPUL010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192452"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="VEJ00_12115"
     CDS             complement(<1..674)
                     /locus_tag="VEJ00_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01951.1"
                     /translation="MKRRLPWYGFFQSVVCHFTALALLFAFTRFFALQPHVVPAPPQA
                     FDHSQVIYYEASEYLPPLDTRSADDPPPQPADPEFSKQPIISVPPGADNRSQTIVTPP
                     KLRIKHDVPLPNIVAWSDKVVKPQLAIPAAPAIPAAEVTRIAPRMETSVVTPPPDSGH
                     LAHRRDQPNLQASVVAPPPELRAARAGPALPGPQTAVIAPPPSVDAAALAVQTVGDLN
                     IGHSAV"
     gene            1008..1535
                     /locus_tag="VEJ00_12120"
     CDS             1008..1535
                     /locus_tag="VEJ00_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01952.1"
                     /translation="MKKQLLLITVGLAVLCVAAPGQDAKSSDKNAGEQATEQKEANQT
                     HSYSGMYTFLKEGEFVQVTVEDEGHVTGFVSRYGDGESDKGAFLDQFFKTGKLDGNKL
                     AFTTEIVHGVWFEFKGTVERGEGKNPGDEAYFVLKGTLTENSTDVNKKVTSHLREVVL
                     KIFPEDAGPIPEPRK"
     gene            complement(1723..2988)
                     /locus_tag="VEJ00_12125"
     CDS             complement(1723..2988)
                     /locus_tag="VEJ00_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXX01953.1"
                     /translation="MQTPREISENRAMHGIAVALLTEDREHLSALQSRLEATRLGQVT
                     FTHLGFPVGPTDAILRQLQDSRAEVVIVDVPTKEPQRAIRAIELIRATTAQIAIFANG
                     EMTEPANIVATMRAGAGEYVDDAAGSDALLEALTRFSSARTRTPGSGGKARIFTFLSA
                     KGGVGCTTAAVNTAMALQQSHGNVVLVDFAPIGHAALHLNLRPQFGVLDALQNLHRLD
                     VSLLDGLMTPTKEGLHLLAGPQQPYPNEPTPGELARLFDLLVNHYRFVVVDASSRLDP
                     TTRLISDLSNAVLVVAQTDVVSLWSAGRIHGFLEEGTGRDRLRVILNRYKKIPGFTDE
                     DVEHVTNCKVLWKVPNAFHTVSPAIDHGAPVVLQEGPEISRAYRALAAALAEASSNSD
                     NGPDLVYGHEGAQKKAPSRLNLSAVRAGQ"
BASE COUNT          742 a          888 c         1219 g          577 t
ORIGIN      
        1 atgaccgcgc tgtgtccgat gttcagatct cccacagttt gcacggcgag cgcggcggca
       61 tccacgctgg gcggcggtgc gatgacggca gtctgcgggc ccggcaaagc cggacctgca
      121 cgggcagccc gcaattccgg aggaggagca accaccgaag cctgcagatt cggctggtcg
      181 cggcgatggg caaggtggcc ggagtcggga ggcggagtga cgaccgaggt ctccatgcgg
      241 ggagcgatgc gcgtgacttc cgcggccggg atggcaggag cagcggggat agcaagctgc
      301 ggttttacga ctttatcgga ccacgccacg atgttgggca atgggacgtc gtgtttgatc
      361 ctaagtttgg gcggggtgac gatggtctgg gagcgattat cggctccggg cggcacggaa
      421 atgatcggct gcttcgagaa ctcggggtca gcgggctggg gcggaggatc gtcggcgctc
      481 cgcgtatcaa gcggaggcaa atactccgag gcttcgtagt agatgacctg ggagtgatcg
      541 aaagcctgcg ggggcgcggg caccacatgc ggctggaggg caaagaatcg cgtgaaggcg
      601 aacagcaatg ccaacgccgt aaagtgacag accaccgact ggaagaagcc gtaccaggga
      661 aggcgccgct tcacgaatac atctggccag aacggtgcgg gagcagagtg caactcaagg
      721 cgcgggagac ggcgcgaaag aagcagatcc cgaaggttcg cgaagaagac tcgcggccag
      781 gacggcaatt cgaccaggag ccggagaaca ggctcggggg cctcattcgg gtcgagaagc
      841 cggagttcaa tcatccgcct atggtagatg caggcggggt gattgggaag ctgggaggcc
      901 gaaaaatgac agagcgtatg ccgggttggg ccaatcggcg aacgggcacg caggacacgg
      961 ggacggccag ccgatagtag tacgattgga tgcagcggag agtcatcatg aagaaacagc
     1021 tattgcttat aactgttggc ttggcggtgc tgtgcgtggc tgcgccaggg caggacgcga
     1081 agtccagcga caaaaacgcc ggggaacagg cgacagagca gaaggaagcg aaccaaacac
     1141 actcctattc ggggatgtac acctttttga aggaaggcga gttcgtgcag gtcacggtcg
     1201 aagatgaggg acatgtgacc ggattcgtgt cgcggtatgg ggacggcgag agcgataaag
     1261 gagcgtttct cgaccagttc ttcaagaccg gaaaacttga cggaaacaag ctagccttca
     1321 cgaccgagat cgtgcacggt gtgtggttcg agttcaaagg cacggtggag cgcggcgagg
     1381 gaaagaatcc cggggacgag gcttacttcg ttcttaaagg cacgctgacg gagaattcca
     1441 ccgacgtgaa caaaaaggta acgtcccatt tgcgggaagt agtgttgaag atatttccgg
     1501 aagacgcagg gccgattccg gagccgcgga agtgagtgag ttagtcccga gccctgagtc
     1561 ccaagtcccg ggtcttgagt gctgagtact gggtaccgag tactcaggtt gatcggaacg
     1621 ctttatcgca caggccgctg agggaacgcg gagaccgcgg agaacaacga gccgggactc
     1681 gggactgaca actcgggact tgggactcgc aactcgggac cgctactgtc cggcgcggac
     1741 ggcggagagg ttgaggcggc tgggagcttt tttctgggcg ccttcgtgtc cgtagacgag
     1801 atcgggtccg ttgtcggagt tcgatgaagc ctcagccaaa gctgcggcca gggcgcggta
     1861 agcacggctg atctcgggcc cttcctgcag gaccacgggc gcgccgtgat cgatagcggg
     1921 cgagacggta tgaaaggcgt tgggaacctt ccacaggacc ttgcagttgg tgacgtgctc
     1981 gacgtcctcg tcggtaaatc caggaatctt cttatagcga ttcaggatga cacgcaggcg
     2041 gtcccgcccg gtgccttctt cgagaaagcc gtgaatgcgg ccggcgctcc agagggaaac
     2101 gacgtcggtc tgggcgacca cgagcactgc gttcgagaga tcggagatca ggcgagtggt
     2161 gggatcgagg cggctggagg cgtcgacgac gacgaagcgg tagtgattga cgaggagatc
     2221 gaacaagcgc gccagttcgc ccggcgtggg ctcgttggga taaggctgct gcggtccggc
     2281 cagcagatgc aggccctcct tggtcggcgt catgagaccg tcgagcagag agacatcgag
     2341 gcggtgaagg ttctgcagag cgtccaacac gccgaactgc gggcgcaggt tgagatgtag
     2401 cgcagcgtgc ccgatggggg cgaaatccac cagaaccacg ttgccgtgcg actgctgcag
     2461 ggccatcgcg gtattgaccg cggcagtggt gcagcccaca ccacccttgg cgctgagaaa
     2521 ggtgaagatg cgggctttgc ccccgcttcc gggcgtgcgg gtgcgcgcgg agctgaagcg
     2581 ggtaagagcc tccagcaagg catcggagcc ggcggcgtca tcgacatatt cgccggcgcc
     2641 ggcgcgcatg gttgcgacga tgttggccgg ttccgtcatc tcgccgttgg cgaagatggc
     2701 aatttgcgcg gtggtggcgc ggatcagctc gatggcgcga atggcgcgtt gaggctcttt
     2761 cgtgggcaca tcaacgatga cgacttcggc acgcgagtcc tgcaactggc ggagaatggc
     2821 gtcggtgggg ccgacgggaa acccgaggtg agtgaaggtc acctggccca ggcgggtcgc
     2881 ttccagacgg ctttgcagcg cggataagtg ttcccggtcc tcggtgagga gggccacggc
     2941 gatcccatgc atggcgcggt tctcgctgat ttctctcggt gtctgcatgt tagaaagaga
     3001 agctatctac gccaagtgac ctaagtaatc gaccgcaacc ggtacccgag ccgacccggc
     3061 gcacagaccg gacccgcaag tgacttggtg gagcagctgg gacgaagaag cgcgcggtac
     3121 aaattcctga aaccaacctg acctctgagt gctcaacatt gtaaggcaca aaagccgcgt
     3181 tggaaaggca cttccgtggg gggtgagaaa aaatccgggc gggaagacgt caggggattg
     3241 caaaaacaag tcagactttc ggactagccc acggcggagc gagcgcgggc gcgttccggg
     3301 tcagcgtctt cttcaggatg ggagtgcagg gcagagacgc gctcaaagca aggagacaat
     3361 tcggcagatt ctggcagata ctcgtaacct tcgagccgca ccgctactcc aattttcgtt
     3421 tccgcg
//
LOCUS       DASPUL010000516         4991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1321969, whole genome shotgun sequence.
ACCESSION   DASPUL010000516 DASPUL010000000
VERSION     DASPUL010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321969"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="VEJ00_12130"
     CDS             complement(<1..1000)
                     /locus_tag="VEJ00_12130"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX01954.1"
                     /translation="MAAATIPVVEAPAARLEHPLRNPNFRMLLTGGTISLLGDQCYLV
                     ALPWLVLQMTGSAVAMGAILMVEAIPRAILMLMGGALSDRISPRKIMMMTATARTVCV
                     GAIGVLVWLHILRLWELYVLSFLFGVADAFSFPAAQAFVPSLVKREQLVAANSVRQST
                     SQLTTIVGPAPAGLAIKALGIAWAFFLDAISFLFIIAALWRLPDPPAPAVAARKRAVL
                     PLILEGLTYVRKDVPLRSLMLLAAVINFCIAGPVGVGLAYLAKTRFGSPAAYGIVVSA
                     AAAGGLLGSLLSGVWRVRRRGMLMISVPVVIGVCLGMLGWLTHLWMVAAVLLLMGAS"
     gene            1129..1773
                     /locus_tag="VEJ00_12135"
     CDS             1129..1773
                     /locus_tag="VEJ00_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXX01955.1"
                     /translation="MRKKTLEPQQARSRESLRKLLKAAAEVMGQHGVEGATIPRIARH
                     AGLTPGSVYRRFHDKDALMEAAILGMLQRQDERMKAGMTPFVTAQIPLPIFAEQVVTG
                     LVVTYRANAAMLRAMKQFAQSRANSPFWKKAAKLEVSHFERVVDMFLAHRDEIKHPDP
                     RTAVSLGLMMVISTLYELVVLPPDLKAWKGLLPQKDQELRQELTRAFLGYLGVA"
     gene            1814..2446
                     /locus_tag="VEJ00_12140"
     CDS             1814..2446
                     /locus_tag="VEJ00_12140"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXX01956.1"
                     /translation="MDIRPSDLAYNDFYRVLISAVAPRPIAWVSTVSASGQPNLAPFS
                     FFNAICAKPPLLAFAPGTRMRKSGDAIRGETKDTLRNVRDTGEFVVNVVTYDAAEAMN
                     LTSGEYDPSVNEFELAKLTLRPSQKVRPPQVAASPLSFECRVYQILDFSSPPHGGSLV
                     IGEIVSIHIEEKNIKDGRLDPDSLDLIGRMGGNQYCRTVQRFEMVRPKVG"
     gene            2526..3884
                     /gene="ffh"
                     /locus_tag="VEJ00_12145"
     CDS             2526..3884
                     /gene="ffh"
                     /locus_tag="VEJ00_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HXX01957.1"
                     /translation="MFENLQEKLQRAFKSLRGQATLTEENIDEALREIRMALLEADVN
                     FKVVKQLIDQIRGKAVGQEVMTALSPGEQVIKIVRDELVEILGKETARMKFASQPPTV
                     VLMAGLQGSGKTTTSGKLAHWFKNGGHRPLLVSVDVYRPAAREQLKIVAEAVKSHIYE
                     GEVSEANTATVERLVKEARREAVVSGCDVLIVDTAGRLHIDEQLMDEMQSLKKLLNPS
                     EILFVADAMTGQDAVNSADEFHKKLSLTGVVLTKMDGDARGGAALSIRQVTGQPIKFI
                     GVGEKYDALEPFHPDRIVSRILGMGDILSLIERAEQQIDKKKAAEMASKALTGDGFSL
                     EDFRDQLRHVKKMGSIKSLVGMMPSIGPFSGLQKAADKVDEKQVDRVEAIINSMTVYE
                     RNHHEVINGSRRKRIARGSGTSVQEVNNLLRQYAQMKKMFKQMGKASFARRLAGMKLP
                     GM"
     gene            3941..4627
                     /locus_tag="VEJ00_12150"
     CDS             3941..4627
                     /locus_tag="VEJ00_12150"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HXX01958.1"
                     /translation="MKWRSLEESAPDLDIRPLREIYAERKELIAKYVPAETLAVHAHA
                     LAELKSRSLAANIVSVGAKGPQFELQDHDGKLVSSSDLLAKRRLVLCFIRGRWCPFCV
                     GQMEAMNLIVPQIEHAGAAFVAISPQTVKQSFFMHDQHRLRFPLLSDVHNDVARQFKL
                     TYRVPSAQEALYRRTFVNLPFTNGDESWELPIPATYILDRDGTVLYASANEDYTERPE
                     PAEIVRVLQN"
BASE COUNT         1096 a         1438 c         1530 g          927 t
ORIGIN      
        1 cactagctcc catcaacagg agcacggctg cgaccatcca caaatgggtt agccatccca
       61 gcattcccag gcatacgccg atcacaactg gcaccgagat catcagcatt ccgcgtctgc
      121 gcaccctcca aactccggaa agcagcgagc cgagcagtcc gcctgccgcg gcagccgaca
      181 ccactatgcc gtatgctgca ggcgatccga accgcgtttt cgccaggtac gccagcccaa
      241 ccccgacggg tccggcaatg cagaagttga tgacagccgc cagcagcatg agtgagcgca
      301 gcggcacgtc tttgcggacg tacgtcaagc cttccagaat taagggcaga actgctcgct
      361 tgcgcgccgc gaccgcaggt gcgggaggat cgggcagacg ccagagcgcg gcaatgatga
      421 acagaaagct gatggcgtcg agaaagaacg cccacgcgat gcccagcgcc ttgatggcaa
      481 gtccggccgg cgccggacca acgatggtcg tcagctggct ggtgctttgc cgaactgagt
      541 tcgcggccac gagttgctcg cgcttgacca aagacggcac gaatgcctgt gctgcgggaa
      601 agctgaaggc atcggccacg ccaaagagaa aactcaggac gtaaagctcc cagaggcgaa
      661 gaatgtgcag ccataccagc actccgatgg cgccgacgca aaccgtacgg gccgtggcgg
      721 tcatcatcat gatcttgcgc ggtgagatgc ggtcactcaa agcgccaccc atgagcatta
      781 gaatggcgcg cggaatcgcc tcgaccatga ggatggcgcc catggcgacc gcggaaccgg
      841 tcatttgcag caccagccag ggcagcgcca ccagatagca ttggtcgccc agcagagaaa
      901 ttgttccgcc cgtcaacagc atgcggaagt tcggattgcg caggggatgt tcgagcctgg
      961 cggctggtgc ttcgacgaca ggaatggtgg cggcggccat gaaaatcgct ccctttcgct
     1021 ccggcgaacc gttagttttc tcgcatcgga acttcgaggt tatgtaaatg taaatttaca
     1081 tttacttttt gtcaagcctg aaatgcaaac ggtgatataa cagcaatcat gcgcaagaaa
     1141 accctggagc cgcagcaggc ccgcagccgc gaatcgctgc gcaagctgct gaaggcggct
     1201 gctgaagtta tgggccagca tggcgtggaa ggcgccacca taccccggat cgcccggcac
     1261 gcaggactca cgccggggtc ggtgtatcgc cgatttcatg acaaagatgc cctgatggag
     1321 gcggcgatcc tgggtatgct gcagcggcag gatgaaagaa tgaaggccgg catgacgccg
     1381 ttcgtcaccg cgcagattcc cctgccgatc ttcgccgagc aggtcgtcac cggtctggtt
     1441 gtgacgtacc gggccaatgc cgccatgctg cgcgcgatga agcaatttgc ccagagccgg
     1501 gccaacagtc cattctggaa gaaggcggcg aagctcgagg tcagccattt cgagcgcgtg
     1561 gtcgacatgt tcctggccca ccgtgacgag atcaagcatc ctgatccccg cactgccgtt
     1621 tctctcggcc tcatgatggt catcagcacg ctgtacgagc tggtggtact cccccctgac
     1681 ctgaaggcgt ggaaagggct gctgccccag aaagaccagg aactgagaca ggaactgacg
     1741 cgggcgtttc taggctatct gggggtcgcg tagtgctaac aaagcaggac gcagactgta
     1801 gtagtgtttt ggaatggaca ttcgcccttc cgaccttgct tacaatgact tctaccgtgt
     1861 cttgatcagc gcggttgcgc cgcgcccgat tgcctgggtc tcgacagtca gcgcgtccgg
     1921 ccagccgaat ctcgcgcctt tctcgttttt caacgccatt tgcgccaagc cgccattgct
     1981 ggctttcgcg ccggggactc gcatgcggaa atcgggagat gccattcggg gagaaacaaa
     2041 ggacacgctg cgcaacgtcc gcgacaccgg agagttcgtc gtcaatgtcg tcacctatga
     2101 cgcagccgag gccatgaacc tgacgagcgg cgagtacgac ccgtcggtga acgaattcga
     2161 gctggcgaag ctgacgctgc ggccttcgca gaaagtgaga ccgccgcagg ttgccgcgtc
     2221 tccgctgagt tttgaatgca gggtctacca gattctcgat ttttcgtcgc cgcctcatgg
     2281 cggcagcctg gtgattgggg aaatcgtttc gatccacatc gaggagaaga acataaagga
     2341 tgggcggctg gatcctgact cactggatct gattgggcgc atgggtggca accaatattg
     2401 ccggacggtg cagcgattcg agatggtcag gccgaaggtc ggctgagatc gatatgcgaa
     2461 cggtaaagtg agaggtatcg tgctctaaag agctcgatta tcgctgttag aataactgtt
     2521 tacctatgtt cgagaatctt caggaaaaac tccagcgcgc cttcaaatcc cttcgcgggc
     2581 aagccacact caccgaggag aatatcgatg aggctctgcg cgaaatccga atggcgctgc
     2641 ttgaagccga cgtcaacttt aaggtcgtca agcaactgat cgaccagatt cgcgggaagg
     2701 ccgtcggtca ggaagtgatg accgcgctct caccgggcga gcaggtcatc aagattgtgc
     2761 gcgacgaact ggttgagatt ctgggcaaag agacggcgcg tatgaagttc gcctcgcagc
     2821 cgccgaccgt cgtgcttatg gctgggctgc agggttccgg caagacgacg acttcgggca
     2881 agttggcgca ctggttcaag aatggcgggc accgtccgct gctggtgtca gtggacgtgt
     2941 atcgtccggc ggcgcgcgag cagttgaaga ttgtggcgga ggcggtgaag agccacatct
     3001 acgaaggcga agtcagcgag gcgaacacgg cgacggttga gcggctagtg aaggaggccc
     3061 ggcgcgaggc cgtggtttcc ggctgtgacg tgctgattgt cgacacggcc ggccgcctgc
     3121 acatcgacga gcagctcatg gatgagatgc agtcgctgaa gaagctgctg aatccgtcgg
     3181 agatcctgtt cgtagccgac gccatgaccg gccaggacgc ggtcaactcc gccgacgagt
     3241 ttcacaaaaa gctgtcgctc accggcgtgg tgcttacaaa aatggacggc gacgcccgcg
     3301 gcggcgcggc tttgtcgatt cgccaggtca cgggccagcc gatcaaattc atcggcgtgg
     3361 gagaaaaata cgatgcactg gagccgttcc atcccgatcg tatcgtgtcg cgcattctgg
     3421 gaatgggcga cattctctcg ctgatcgagc gtgccgagca gcagatcgac aagaaaaagg
     3481 ccgcagaaat ggccagcaaa gcccttaccg gcgacggctt ctcgctcgaa gattttcgtg
     3541 accaactgcg gcacgtgaag aaaatggggt cgatcaagag cttggtgggc atgatgccgt
     3601 cgattgggcc gttctccgga ttgcagaagg cggccgacaa ggtggacgag aagcaggtcg
     3661 atcgtgtcga ggcaatcatc aactcgatga ccgtatacga gcgcaatcat cacgaggtga
     3721 tcaacggcag ccgccgcaag cgaatcgccc gcggctcggg caccagcgtg caagaggtga
     3781 acaacctgct ccgccagtac gcgcagatga agaagatgtt caagcagatg ggcaaagcga
     3841 gcttcgcgag aaggctggcg gggatgaagc tgccgggaat gtagaagaac agtcgtcggc
     3901 tctcagccat cagccttcag ccgacaccat gcatccgacc atgaaatggc gctcgctcga
     3961 agaatcggct cctgatctcg atattcgtcc cctgcgcgaa atttacgctg aacggaaaga
     4021 gctgattgcg aaatacgtcc ctgcggagac attggctgtt catgcgcacg ctctcgccga
     4081 gctgaaatca aggagtctgg ccgcgaacat tgtgtctgtc ggagccaaag ggccgcaatt
     4141 cgaactgcag gatcacgacg gcaagcttgt ttcttcttcc gatcttctag ccaagagacg
     4201 cctcgtcctt tgcttcatcc gcggccgctg gtgcccattc tgcgtgggac agatggaggc
     4261 aatgaatctg atcgttccac agatcgagca tgccggggcc gcgttcgtcg ccatttcgcc
     4321 gcagaccgtg aagcaatcct tctttatgca tgatcaacac cggctgcggt tcccgctgct
     4381 gtctgacgtg cacaacgacg tcgcacggca gttcaagcta acgtatcgcg tgccatcggc
     4441 gcaggaggcg ctgtatcggc gcacgttcgt caatcttcct ttcaccaacg gcgatgagag
     4501 ctgggaattg ccgatcccgg cgacgtatat tctcgaccgc gacggaactg tgctttacgc
     4561 gtcagcaaac gaggactaca cggagcggcc tgagccagcg gaaatcgtgc gagtcctcca
     4621 gaattgattc caggaagaat cgattacacc gcggtggaag agcggccctt cagggccgcg
     4681 ttcagcgcgg cgttcgaaga ggccttcagc ccgggtggtc gtttctctca ggggctaaag
     4741 ccgatcgaat tttacgatcc gtacgcgcgg cgctgaagcg ccgctcttcc acggaatttc
     4801 catcgcccgc cggtccgcct gccgcagccg gcaaagccga acgacgccct cgccattcca
     4861 ttacactact accgcatgca ttacatctac ggcatcaatg ccgtcaccga ggcgctgagg
     4921 gcgcacggcc gcgcgttcga gtgggtgggc atggcgaagg aacgccacga cctgcggctg
     4981 cagcgactga t
//
LOCUS       DASPUL010000517         3926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1871531, whole genome shotgun sequence.
ACCESSION   DASPUL010000517 DASPUL010000000
VERSION     DASPUL010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1871531"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..358
                     /locus_tag="VEJ00_12155"
     CDS             2..358
                     /locus_tag="VEJ00_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HXX01959.1"
                     /translation="MDKASPDVGTGLVGAPECGDVMKLQIKVNRETNVIEDAKFKTFG
                     CGSAIASSSLATEWVKGKTIDEALAIKNTEIVKELSLPPVKIHCSVLAEDAIRAAIGD
                     WKKKQESDKPVAAQAK"
     gene            480..851
                     /locus_tag="VEJ00_12160"
     CDS             480..851
                     /locus_tag="VEJ00_12160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HXX01960.1"
                     /translation="MTTAIEQPGTAAAKGITVTDKAIAKIRSAMAKECISPEQGGLRL
                     GVQGGGCSGLSYNIRFDTQPRERDRIFQFGDVRVFVDPKSFIYLHGMTLDYQETLMQQ
                     GFVFVNPQATKSCGCGTSFSA"
     gene            981..1739
                     /gene="hscB"
                     /locus_tag="VEJ00_12165"
     CDS             981..1739
                     /gene="hscB"
                     /locus_tag="VEJ00_12165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HXX01961.1"
                     /translation="MTKSALTAIHARPLPEETHSCWSCGAMLAAHFCASCGRVQPPQP
                     VDYFTFFGLPPKLNIDVAALEKDFYELSRKLHPDLNATTGSQEQEWSLEQSSMLNDAY
                     RTLRDPIKRTQYLLHLEGIHLEEQSKSATEQARSTGEIKKQIVPPDLLEEVFELNMQL
                     EELRANKKLGEDDPALVAEIAKHKATLETKHEALLQELQEYWADWDSLIERNHGSKAP
                     AEDRAKITSKMLDVLNRRSYIRNLLRDVNEALEQ"
     gene            1751..3652
                     /gene="hscA"
                     /locus_tag="VEJ00_12170"
     CDS             1751..3652
                     /gene="hscA"
                     /locus_tag="VEJ00_12170"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF003520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HXX01962.1"
                     /translation="MASDHIVGIDLGTTNSLVAFMQGETPVVIPGEDGSNLLPSVVAL
                     DAANQPVIGNPARNYLIETPERAVYSIKRLMGRGMEDIQEELKLFPFRLADDLQPGEV
                     LRIKLGGKTFTPPEISALILRQLKRNAERYFGGPVTKAVITVPAYFNDAQRQATKDAG
                     RIAGLDVLRLVNEPTAASLAYGLDKTQNGIVAVYDLGGGTFDISILKLHDGIFEVIAT
                     NGDTHLGGDDIDNLLITIALADIGGDLGLDLRRNAEVVQAIRKAVIDAKIALSSQPSV
                     KLDIELPGGERYQREITRERFEQLIQPIIDRTVGPCKQAMKDANLKPEQIDEVVLVGG
                     STRIPKVRALVKEMFHREPHTDLNPDEVVALGAAVQANILGGGSEATQNMLLLDVTPL
                     SLGIEVAGGVTDKIILRNSTIPASATQFYTTQVDGQANVAIHVLQGERELAKDCRSLA
                     RFDLKGIPPMAAGIPRVEVKFLIDANGILHVSAREQRSGKEAEIQVQPSYGLTDEQVE
                     SMILDSFDNAEEDFRRRQLIEARNEAATILAALEKGKKSPAWGQLTTDEKKQIAKMEK
                     ALVVVNTEDDYAAIRKAIDALNQGTMRLAELMMDTAVSSALKGKTMDEADLGEGPTAP
                     HPMAKAEFE"
     gene            3661..>3926
                     /locus_tag="VEJ00_12175"
     CDS             3661..>3926
                     /locus_tag="VEJ00_12175"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXX01963.1"
                     /translation="MAAKLTYVIEFVADMDRAVKFYRDTMGLPLTFQSPGWSEFATGE
                     TKLGLHPASEKNPAGKIEIGFGVPDLAKFYEEMRAKGVKFPMPP"
BASE COUNT          880 a         1131 c         1205 g          710 t
ORIGIN      
        1 gatggacaag gcgagtccgg acgtgggcac cggcctggtc ggcgcgcccg agtgcggcga
       61 cgtcatgaag ctgcagatca aggtcaatcg cgaaaccaac gtcattgaag acgccaagtt
      121 caagaccttt ggatgcggct ctgccatcgc ctcgtcttcg ctggccaccg agtgggtcaa
      181 gggcaagacg attgacgaag cactggcgat caagaacacc gagatcgtga aggagctttc
      241 gctgcctccg gtgaagattc actgctcggt gctggctgag gacgcgatcc gcgccgcgat
      301 cggtgactgg aagaagaagc aggagagcga caagccggtc gctgcgcaag ccaagtagct
      361 tctgctgact gtcatcctga gcgcagcgaa ggacctatgc aacctgccag cggcatcgat
      421 agcgcctgca aacacataga tccttccctt cgctcaggat gacaagacga tttgacgtca
      481 tgactaccgc catcgaacaa cccggaacgg ccgccgcgaa aggcatcacg gtgaccgaca
      541 aggcgatcgc caagattcgc agcgcgatgg ccaaggagtg catttcgccc gagcagggcg
      601 ggctgcgcct cggggtgcag ggtggcggat gttccggctt gagctacaac atccgcttcg
      661 acacccagcc gcgcgagcgc gatcgcatct tccaatttgg cgatgtgcgg gtctttgtgg
      721 atccgaagtc gttcatctac ctgcacggca tgaccctgga ctaccaggaa acgctgatgc
      781 agcagggctt cgtcttcgtg aatccgcagg ccacgaagtc ctgtggttgc gggacttcgt
      841 tctccgcctg agtacgagga agtcaaaccg ccgaggacgc tgagtgcgcc gagaaaagac
      901 cggggttctt cttccttggt ttctctgcgt tccctgcggg ctcggcgata aattttctgt
      961 gacctgagaa tcagaatgtg atgaccaaat ctgctttaac cgcaattcac gccagacctc
     1021 tgccggagga aacccattcc tgctggtcgt gcggagccat gctggcggcg catttttgtg
     1081 cgtcatgcgg gagggtgcaa cctccgcagc ccgttgacta cttcacgttt ttcggcctgc
     1141 cgcccaagct caacattgac gtggctgccc tggagaagga tttctacgag ctgagccgca
     1201 agctgcatcc cgacctgaat gccacgacgg gcagccagga acaggaatgg agcctggaac
     1261 agagctccat gctcaacgac gcctaccgca cgctgcgcga tccgatcaag cggacgcaat
     1321 acctgctgca tctggagggg attcatctcg aagagcagtc aaagagcgcg accgagcagg
     1381 ctcgctcgac cggagagatc aagaaacaga ttgttccgcc cgacctgctc gaagaagttt
     1441 tcgagctcaa catgcaactg gaggagctgc gcgccaacaa gaaactgggc gaggacgatc
     1501 cggctttggt cgccgagatc gccaagcata aagccactct cgagaccaag cacgaggcgt
     1561 tactgcagga attgcaggaa tattgggcag actgggacag tctcatcgag cgcaaccatg
     1621 gttcgaaggc cccggcggag gatcgcgcaa agatcacgag caagatgttg gacgtattga
     1681 accgccgcag ctacatccgg aatctgctgc gggatgtgaa tgaagcgctg gaacagtgag
     1741 tacttcgaga atggcatcgg atcatattgt cgggatcgat ctgggaacga cgaactcgct
     1801 cgtggccttc atgcaggggg agacgcctgt cgtgattccg ggcgaggacg gatcgaacct
     1861 gctcccgtcc gtcgttgcgc tcgatgcggc gaatcagccg gtgattggca atccggcccg
     1921 caattatctg atcgagacgc cggagcgcgc cgtctactcg atcaaacgtc tcatgggacg
     1981 cggcatggaa gacattcagg aagaactgaa gctctttcca tttcgcctgg cagatgacct
     2041 gcaaccgggc gaggtgctgc gcatcaagct gggcggcaaa acgttcactc cgccggagat
     2101 ctccgcgctc attcttcgcc aactcaagcg caatgcagag cgatatttcg gtggaccggt
     2161 cacaaaagca gtcatcacgg ttccggctta cttcaacgat gcgcagcggc aagccacgaa
     2221 ggatgccggt cgtattgccg ggctcgatgt cttgcgactg gtgaacgagc cgaccgcggc
     2281 gtcgctcgcc tacgggctgg acaagacgca aaatggaatc gtggcggtct atgatctggg
     2341 cggaggcacg ttcgacatct ccattctcaa gctgcacgac gggatcttcg aggtcatcgc
     2401 taccaacggc gacacgcacc tcggcggcga cgacatcgac aacctgctga tcacgattgc
     2461 gctcgccgat attggcggcg acctcggtct cgacctgcgc cgcaatgccg aggtggtaca
     2521 ggcaattcgc aaggcagtga tcgatgccaa aatcgcgctt tcttcgcagc cctcggtcaa
     2581 gctcgacatc gaactacccg gtggcgagcg ctaccagcgt gagattacgc gcgaacggtt
     2641 cgagcaactg attcaaccca tcatcgatcg cacggttgga ccctgcaagc aggcgatgaa
     2701 agatgccaac ttgaagcccg agcagatcga cgaagtcgtg ctggtcggcg gctcgacccg
     2761 catcccgaag gttcgtgcgc tcgtgaagga gatgttccac cgcgagccgc acacggatct
     2821 gaatcccgac gaggtcgtgg ctctcggcgc ggcagtgcag gcgaatattc tcggcggagg
     2881 ctccgaagcg acccagaaca tgctgctgct cgacgtcacg ccgctatcgc tcgggattga
     2941 agtggccggg ggcgtcaccg acaagattat cctgcgcaac tccaccattc ccgcctcggc
     3001 tacgcagttc tacacgactc aagtcgacgg ccaggccaac gtcgccattc acgtgctgca
     3061 gggcgagcgc gagctggcca aagactgccg ctcgctcgct cggttcgatc tcaagggcat
     3121 tccgcccatg gcggcgggca tcccgcgcgt cgaggtcaag ttcctgattg acgcgaacgg
     3181 cattctgcac gtttccgcgc gagagcagcg cagcggcaag gaagccgaga ttcaggtgca
     3241 gcccagctac gggctgaccg acgagcaggt cgagagcatg attctcgact cgttcgacaa
     3301 cgccgaggaa gacttccggc ggcgtcagct gattgaggca cgcaacgaag ctgcaacgat
     3361 ccttgccgcg cttgagaagg gcaagaagag cccggcctgg ggccagctca cgaccgacga
     3421 gaagaagcag atcgccaaga tggagaaggc tctggttgtg gtgaataccg aagacgacta
     3481 tgcggcgatc cgcaaagcga tcgacgcgct gaaccagggc acgatgcggc tggcggaatt
     3541 gatgatggac acagccgttt cgtcggcgct gaaaggcaag accatggacg aagccgatct
     3601 gggcgaaggc ccgaccgcgc cgcatcccat ggccaaagcg gaatttgaat aggggactat
     3661 atggcggcga agctgaccta cgtgattgag tttgtagcag acatggaccg cgcggtgaag
     3721 ttctatcgcg acaccatggg tttgccgttg acctttcaat cgcccggatg gagtgagttt
     3781 gccaccgggg aaaccaagct gggactgcat cccgcgtcgg agaagaatcc ggcaggcaag
     3841 attgaaattg gattcggcgt gcctgatcta gcgaaatttt atgaagagat gagagccaag
     3901 ggagtgaagt ttcccatgcc cccgaa
//
LOCUS       DASPUL010000518         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1742465, whole genome shotgun sequence.
ACCESSION   DASPUL010000518 DASPUL010000000
VERSION     DASPUL010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742465"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VEJ00_12180"
     CDS             <1..225
                     /locus_tag="VEJ00_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01964.1"
                     /translation="HAGTRYGWFASYANTNVGRIVVVVFLEGGRPTFGPKAAEIAGRM
                     YRSMYDHNFFVAGPGAPADQATAKPDTATP"
     gene            464..2167
                     /locus_tag="VEJ00_12185"
     CDS             464..2167
                     /locus_tag="VEJ00_12185"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXX01965.1"
                     /translation="MMPQEVFDWGLMISVGRGMSSAGVLALIAVASGGVILIAGRRYC
                     QRLGWRIPGQDGQLSFHTAAQAAPAVLWTARPDGSVDFVSDQLYAYSGLTRGQVLNWG
                     WTEALHPDDLPACKERWERSLREGAAYEIEYRLRRHDGVYRWFLVKGNPLRDENHRVI
                     RWIGTTSDIEDQKHHQQMLEQQIHERTVELADANTRLQEEMVERDYARRKLDEQNERI
                     MRDLTERSQRATLLAKMGEHMQSCITQDEVFAAALGYAPKVFPTSPGAVALFNSGRNL
                     VEIAGQWLDCQLPITVFEPNACWALRTGHPHLVVAGDTTAPCAHAAGVKNTYLCVPIL
                     AQGEALGVLHFQATDEAPTLADSELSFKTTFAGQVGLSVANIRLREALRAQSIRDPLT
                     GLYNRRYLTEMLERETRRAIRSEQSLGVLMLDLDHFKKFNDTYGHEAGDRVLRETASL
                     LMKSVRAEDIVCRFGGEEFVVILPMADLQVTEARGERIRSRTRELTVLHQGQSLGMIT
                     VSVGVAVLPEHGTNPKELLEAADAALYRAKREGRDRVVVAEITAGADALVASLVESKK
                     S"
     gene            complement(2180..>2621)
                     /locus_tag="VEJ00_12190"
     CDS             complement(2180..>2621)
                     /locus_tag="VEJ00_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXX01966.1"
                     /translation="KEIGFVFQTFNLLARATALHNVELPLIYAGIPTEERLERARASL
                     TAVGMEERMSHKPNELSGGQRQRVAIARALVNKPSIILADEPTGNLDSQTGLEIMALF
                     DRLHAEGNTIILVTHEHDIAEYAHRVIHIKDGVIASDESKSQQN"
BASE COUNT          469 a          758 c          885 g          509 t
ORIGIN      
        1 cacgccggga cgcgttacgg ctggttcgct tcctacgcca acaccaacgt cgggcgcatt
       61 gtggttgtgg tcttcctcga aggcggacgg cctactttcg gccccaaagc ggctgagatt
      121 gccgggcgca tgtaccgcag tatgtacgat cacaacttct tcgtcgccgg gcccggggcg
      181 ccggcggatc aagccacggc gaagccagat acagccactc cgtaattcga ttacaacttt
      241 tcctgaagag gcgcggcctg gctgcgcctt cttcgttttg ggctcgatct tcgtagggtc
      301 gcgttagcgc tcgctcactc gaatacggtt ttagcgacct ctaatgcagg agtcagagtc
      361 gagcgagctt ttcgcttccg acgtcacagc gattcctcac cagaatcgac tttctttttt
      421 ttctgccgat aaggaatgaa gtgggagaac tacgtcagca acgatgatgc cgcaggaagt
      481 tttcgactgg gggttgatga tttcggtcgg gcggggaatg agctcggcgg gagtgcttgc
      541 cctcatcgcc gtcgcttcgg gcggcgtgat cctgattgcc ggccggcggt actgccagcg
      601 gttaggctgg agaattcccg gccaggatgg gcagctttcc tttcacacgg cggcgcaggc
      661 tgctcccgca gtgctgtgga cagcacggcc ggatggctcg gtcgactttg tcagcgatca
      721 gctctatgcg tatagcggct tgacccgggg gcaggttctt aactggggct ggacggaggc
      781 gctgcatccc gacgatctcc ccgcctgcaa ggagcgctgg gaacgtagtc tgcgcgaagg
      841 cgcggcgtat gaaatcgaat accgtctgcg ccgccacgac ggtgtctatc gctggttcct
      901 ggtgaaaggg aatcctctcc gcgatgagaa tcaccgggtg atcaggtgga tcggaacgac
      961 gtcggacatc gaagaccaga agcaccacca gcaaatgctc gaacagcaaa tccacgagcg
     1021 caccgtagaa ttggccgacg ccaatacgcg gctgcaggaa gagatggtgg aacgcgatta
     1081 cgcccggcgc aaactcgacg aacaaaacga gagaatcatg cgcgacctca ccgagcgctc
     1141 gcagcgggcc acgttgctgg ccaagatggg ggagcacatg cagagctgca tcacccagga
     1201 tgaggtgttc gcggcggctc tggggtacgc gcccaaggtc tttcccacca gccccggggc
     1261 agtggcgctg ttcaacagcg ggcgcaatct ggtcgagatc gcggggcagt ggctggactg
     1321 ccagctcccg atcaccgtct ttgagccgaa cgcctgctgg gcgctgcgca ccggccaccc
     1381 tcatctggtc gtggccggcg atacgaccgc gccttgcgcg cacgcggcgg gggtgaagaa
     1441 cacgtatctt tgtgtgccga ttctggcaca gggcgaggcc ctgggtgttt tgcacttcca
     1501 ggcgaccgac gaggcgccga ctctggcgga ctcggagctg tcgttcaaga ccacctttgc
     1561 ggggcaggtt gggttgtcgg tagccaacat ccggctgcgc gaagcgttgc gggcgcagtc
     1621 cattcgcgac ccgctgaccg ggctatataa ccggcgctac ctgaccgaga tgctggagcg
     1681 cgaaacccgg cgggccatcc gctccgagca atcgctcggc gtgctgatgc tcgatctcga
     1741 ccacttcaag aagttcaacg acacttacgg gcacgaggcc ggcgaccggg tgctgcgcga
     1801 gaccgcttcg ctcctgatga agagcgtgcg cgcggaagac atcgtctgcc gcttcggggg
     1861 cgaggagttt gtggtcattc tgccgatggc ggacctgcag gttacggagg ctcggggcga
     1921 acgaattcgc tcccgcacgc gcgagctgac cgtgttgcat caggggcagt cgctggggat
     1981 gattacggtc tcggtagggg tggcggtgct tcctgagcac ggcaccaatc ccaaggagct
     2041 gctggaagcc gcggacgcgg cgctttaccg ggccaaacgg gaaggccggg atcgcgtagt
     2101 ggtagcggag attacagcgg gcgccgatgc gctggtggcg agcctggtcg aaagcaagaa
     2161 gtcgtgacgg cgtgagcgct cagttctgct ggctcttgct ctcgtcgctg gcgataacgc
     2221 catccttgat gtgaatcacg cgatgagcgt attcggcgat gtcgtgctcg tgcgtgacca
     2281 ggatgatggt gttgccttcg gcgtgcaggc ggtcgaacag cgccatgatc tccaagccag
     2341 tttgcgaatc gaggttgccc gtgggctcgt ccgccaggat gatcgagggc ttgttcacca
     2401 gagcgcgggc gatggcgacg cgctggcgct ggccgcccga gagctcgttg ggcttgtggc
     2461 tcatgcgctc ttccatgcct actgcagtca gggaagcgcg ggcgcgctcc aggcgctctt
     2521 cggttgggat gccggcgtag attaacggga gttccacatt gtgcagggcc gtggcccggg
     2581 ccagcaggtt gaaggtctgg aagacgaatc cgatttcctt g
//
LOCUS       DASPUL010000519         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_368993, whole genome shotgun sequence.
ACCESSION   DASPUL010000519 DASPUL010000000
VERSION     DASPUL010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368993"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VEJ00_12195"
     CDS             <1..412
                     /locus_tag="VEJ00_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01967.1"
                     /translation="SCCEAAGLWPDAVLSGHAHLYQRFTRTVNGRAVPYIVSGSGGFA
                     ATAPMVAAPPAGTVIGDHKLEVDPIIEFGYLTVNTNAKTLTVTFKTAMRGKPVTAVDF
                     VTLDLVAGRITASGNPATAKKAGKAGRKAAKKKR"
     gene            complement(522..1544)
                     /locus_tag="VEJ00_12200"
     CDS             complement(522..1544)
                     /locus_tag="VEJ00_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXX01968.1"
                     /translation="MKAVRIHQFGGPEVLTYEDAPDPQPRKDQVLIRVKACALNHLDL
                     WVRKGLPGVKLPHILGSDIAGEVVESGEYVTGFKPGQRVLIAPMHYCGHCAACVAGVQ
                     NQCREFTVLGNTVDGGNCELIAVAPANVIPIPDALDFNQAASVPLVFLTAWHMLVGRA
                     GVRPGQTVLVLGASSGVGIAAIQIAKLFHCRVITTAGDATKLEKARALGADYGIDHYK
                     QKISEEVRRITKKEGVDIAVEHVGAATWDESVKSLKSGGTLVTCGATTGPSVGVDLRH
                     LFARQLSLLGSYMGTMGELHEVLKHVFAGRLKPVVDRTFPLKDLRAAHEHMEKSQMFG
                     KIVVNP"
     gene            1831..2697
                     /locus_tag="VEJ00_12205"
     CDS             1831..2697
                     /locus_tag="VEJ00_12205"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HXX01969.1"
                     /translation="MDIYEQIVELRRQGRRGAVATIVNVRGSIPSFRTAKMLVRDDGS
                     IAGTIGGGCVEAEVWQAAREVMESEKPRTLTFDLNQDPKYDTGLVCGGTLEVFVEPVL
                     PPALLYVFGAGHVARSLCHTATPAGFDVIVTDDRSSYATPERFPAAREVHALEFDEAM
                     KKFDPNESSYIVIVTRGHRDDLRILRWAVQTRSRYVGMIGSKRKVIGIFNTLREEGLP
                     AQLFERVHAPIGLDIGASTPEEIAVAITAELIAVRRHATAALPHMSWFRGERTDETAN
                     QVAETPEDTCQQ"
     gene            2780..3409
                     /locus_tag="VEJ00_12210"
     CDS             2780..3409
                     /locus_tag="VEJ00_12210"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXX01970.1"
                     /translation="MSRSPSFAGVILAAGESSRMGTEKALLPWPPQAAGQRPTGETFL
                     SAAIRSLLSTDFVVVVAGANEAALTPIVYANGAFVAVNPDPSRGQFSSLQVGLHEVLN
                     RGRDAALITLVDRPPVREATVEALRDAFASAPENVWAVVPEYSGQHGHPYVAGRELIE
                     AFLQAPPTATAREIEHRHQEHIQYLAVADPFVAANVNTPEEYAALLTKA"
BASE COUNT          661 a         1066 c         1079 g          670 t
ORIGIN      
        1 ctcctgctgt gaggcggcag gcctgtggcc agatgccgtg ctttcgggcc acgcgcatct
       61 gtatcaacgc ttcacgcgaa cggtgaacgg acgtgcagtg ccttacatcg tttccggttc
      121 aggcggattc gcggccacgg ctccaatggt tgccgcgccc ccggcgggaa cggttatcgg
      181 cgaccacaag ctggaagtcg atcccatcat cgagttcgga tatctgaccg tcaacacgaa
      241 cgcaaagact ctgactgtca cgttcaagac tgcgatgcgg ggaaaaccgg tgacggcggt
      301 ggatttcgtc acgctcgatc tggtggccgg gagaattacg gccagcggca atccggcaac
      361 ggcaaagaaa gccgggaagg ctggccggaa ggcggcgaag aagaaacggt gagaaccgcc
      421 caggactacg accaccgggg ctgaagcccc ggaatacttc ggagctctac gcggcgctca
      481 agcgccgctc ctccacggtg tcgagtcgcc gtgtctagag cttaggggtt aacgacgatc
      541 ttcccgaaca tctgactctt ttccatgtgc tcgtgggccg cgcgcaggtc tttcagcggg
      601 aacgtgcggt ccacgaccgg ttttaggcga ccggcgaaga cgtgcttcag cacttcgtgc
      661 aactcgccca tcgttcccat gtaggagccg agcaggctca actgccgggc aaaaagatgg
      721 cgcagatcga ctcccacgct ggggccagtg gtagccccac aggtgaccag tgtgcctccc
      781 gatttaagcg acttgacgct ttcatcccag gtggcagcgc cgacgtgttc gactgcgatg
      841 tcgacgcctt cttttttggt gatcctgcga acttcttcag aaatcttctg cttgtagtgg
      901 tcgatgccgt agtcggcgcc cagcgctcgc gctttttcaa gcttggtggc gtcgccggcg
      961 gtggtgatca cgcggcagtg gaatagcttg gcgatctgga tggcggcgat gcccacgccc
     1021 gagctggcgc ccagcacgag caccgtttgt cccggacgga cgcccgcgcg cccaaccagc
     1081 atgtgccacg ccgtgagaaa caccagcggc acgctggccg cttgattgaa atcgagcgcg
     1141 tcgggaatgg gaatgacatt ggccggcgca accgcaatca gttcgcagtt gccgccgtcg
     1201 actgtgttgc cgaggaccgt aaactcgcgg cactgattct gcactccagc gacgcaggcg
     1261 gcgcaatgtc cgcagtagtg catgggagcg atcagcacac gttgcccggg cttgaatccg
     1321 gtgacgtatt cgcccgactc aaccacttcc ccggcgatgt cgctgcccag aatgtgcggc
     1381 agcttcacgc cggggagccc tttgcgcacc cacaggtcga gatgattcag ggcgcaggcc
     1441 ttcacccgga tgagcacctg atctttgcgt ggctgcggat cgggggcgtc ttcgtatgtg
     1501 aggacttccg ggccaccgaa ttgatgaatg cgaacggctt tcatgagact tcctccgcgg
     1561 caacaggtga cgctaccaca aagttcgtca gcgttggaat tggtcaccct ggtcggtagt
     1621 ggggcagtat actcagggtt ttcttggcgg ccttcgcgcc ctcttggcga tctttgcgtt
     1681 caacagctcc ttaccgccag gttcggaaag aagtaccgca aagaacgcta agctgccgcg
     1741 gccgcaaatg cggggcattt cccctccgtt tgactctcac ttgttaaccg actgataatc
     1801 attcgtggca agctggcgcg accgacacca gtggacatct acgaacaaat cgttgaactt
     1861 cgacgccagg ggcggcgcgg tgcagtggcg accatcgtca acgtgcgcgg atcgatcccg
     1921 tcattccgca cggccaagat gctggtgcgc gatgacggct cgattgcggg cacgatcggc
     1981 ggcggctgcg tcgaggccga ggtctggcag gcggcgcgcg aggtcatgga atcggagaag
     2041 ccgcgcaccc tgaccttcga tctgaatcag gatccgaaat acgacaccgg cctggtctgc
     2101 ggcgggacgc tcgaggtctt cgtcgaaccc gtcctacctc cagcattact ttatgtgttc
     2161 ggtgcaggtc atgttgcacg aagcctttgt catacagcaa caccagcagg cttcgacgtg
     2221 attgtgaccg acgatcgatc ttcttacgcg actccggagc gatttcccgc cgctcgcgaa
     2281 gtccacgccc tcgaattcga cgaggccatg aagaagttcg atcccaacga atcttcctac
     2341 attgtgatcg tgacgcgcgg acatcgcgac gacctgcgca tcctgcgctg ggcggtacag
     2401 acgcggtctc gctacgtggg catgatcggc tcgaagcgga aggtgatcgg gatcttcaac
     2461 acgctccgcg aggagggcct gccggcgcag ttgtttgagc gcgttcatgc gcccatcggg
     2521 ctcgatatcg gcgcgagcac tccggaagag attgccgttg ccatcacggc ggagctgatc
     2581 gcggttcgcc ggcacgccac cgcggctctg ccccacatga gctggttcag gggcgagcgg
     2641 accgacgaaa ccgcaaatca ggtcgccgag acaccggaag atacctgcca gcagtgattt
     2701 tccgatttac aattgcgatt gcggattgaa gccacggccc gttcaagcgg caaatccaca
     2761 accggaattc ggaaacccca tgtcccgctc gcccagcttc gccggagtga tccttgccgc
     2821 aggcgagtct tcgcgcatgg gcacggaaaa ggcgctgctg ccatggcccc cgcaagctgc
     2881 gggccagcgc ccgacgggcg agacgtttct ctcggcagcg attcgttccc tgctctccac
     2941 ggacttcgtc gtggtcgtgg ccggggcgaa cgaagcggcg ctgacaccga tcgtttatgc
     3001 caatggcgca ttcgtcgcgg tcaatcctga ccccagccgc ggccagttca gttcgctgca
     3061 ggtcggcttg cacgaagttc tcaaccgcgg acgcgacgcg gccctgatca ctctggtcga
     3121 tcgcccgccg gtgcgcgagg ctacggtcga ggctctgcgt gatgccttcg ccagcgcacc
     3181 ggaaaacgta tgggcggtcg tccccgagta ttccgggcag cacggccatc cttacgtcgc
     3241 tgggcgggag ctgatcgagg cgtttctgca ggcgccgccc acggcgacgg cgcgcgagat
     3301 cgagcaccgg caccaggagc atattcaata tctcgcggtg gcagatccgt tcgtggccgc
     3361 gaatgtgaac acgccggaag agtacgctgc gctgctgaca aaagcgtaag tacctcacat
     3421 cttgacgcag atttgagatt gcagatttca gattgaccgg acctcgcgta gatctg
//
LOCUS       DASPUL010000520         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2018948, whole genome shotgun sequence.
ACCESSION   DASPUL010000520 DASPUL010000000
VERSION     DASPUL010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2018948"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VEJ00_12215"
     CDS             complement(<1..725)
                     /locus_tag="VEJ00_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01971.1"
                     /translation="MRSYILIRLIPVLLCTALCLLAPSHAPGQEAPYFVTYTHYLEEP
                     DNLEVAANSTFATQRGGNRTLAYWSELEYGVTGWWTTEFYLDGQTTFHDSTVFTGFRW
                     ENRFRPLKREHWINPLLYVEFENVNGADKILKEVEGHDVETDFASSNAEGRSERQREL
                     ELKLILSSSFKGWNVSENLLFVKNLSNEPWEFGYAWGISRPLALKASARKCSFCAENF
                     VAGVEMYGGLGDRYRFGLQETSH"
     gene            885..1355
                     /locus_tag="VEJ00_12220"
     CDS             885..1355
                     /locus_tag="VEJ00_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX01972.1"
                     /translation="MRSSLKVLLLRIVDEGYARKTWHGPNLRQSLRGVTAKQAAWRPG
                     FGRHNIWELTLHAAYWKYAVRRRIEGGKRGLFALGGSNFFARPQKGGLNEAAWRADKE
                     LLEKEHRALRAAIERAFRTGRAQRFMNQICGIAFHDVYHAGQIRLLRRLQEKSK"
     gene            complement(1357..2034)
                     /locus_tag="VEJ00_12225"
     CDS             complement(1357..2034)
                     /locus_tag="VEJ00_12225"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXX01973.1"
                     /translation="MPQANSPLLCYVTDLRALAGAPQMAAAEGERGLLDVIERVAAAG
                     VQWIQIREKDLPAARLATLVRAARRQCPPSARIVVNDRLDVVLSAGADGIHLGEMSLP
                     PAGVVALLRERKSPGPGPESLLVGVSTHSLDAARAAELAGAAYVFFGPVFETPSKRIY
                     GAPQGMERLAEVCRRVRIPVLAIGGITAANARQCFAVGAAGIAAIRLFQESADPSRLV
                     RSLLSEL"
     gene            complement(2018..>2240)
                     /locus_tag="VEJ00_12230"
     CDS             complement(2018..>2240)
                     /locus_tag="VEJ00_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01974.1"
                     /translation="VVLICFEIGAVLLYLPWSAFWEQNYFLLHYPSLIPVMLNFFVRG
                     TVSGIGVLDIVLAARMIRRSPRATRASSQ"
BASE COUNT          450 a          720 c          674 g          396 t
ORIGIN      
        1 tagtgcgacg tctcctgcag gccgaagcgg tagcggtccc ccaggccgcc gtacatctcg
       61 accccggcca caaagttctc cgcgcagaaa ctgcactttc gcgccgaggc tttcagcgcg
      121 agcggccggc tgattcccca cgcgtaaccg aactcccacg gctcgtttga gaggttcttc
      181 acgaacagca ggttctcaga gacgttccat cccttgaagc tgctcgaaag aatcagcttc
      241 aattccaatt ctcgttgccg ctcggaccgt ccctcggcat tcgacgaagc aaagtcggtc
      301 tcgacgtcgt ggccttccac ttctttcagg attttgtccg ctccgttgac gttctcgaac
      361 tccacgtaca gcagcggatt gatccagtgc tcgcgcttca gcgggcgaaa acggttctcc
      421 cagcggaagc cggtaaagac cgtactgtcg tgaaaggtcg tctggccgtc gagatagaac
      481 tccgttgtcc accaccccgt caccccgtat tcgagttccg accagtaggc aagcgtgcga
      541 ttgccaccgc gctgcgtggc aaacgtggag ttcgccgcga cctcgagatt gtccggctcc
      601 tcaaggtagt gggtgtaggt gacaaagtag ggagcctcct gccccggggc gtggctcggg
      661 gcgagaaggc agagggccgt acagagcaga acaggaatca atcggatcag gatataagac
      721 ctcacgagtc ctctttgcaa atgatttgca actaggtcgt gagcctacca cggccttcgt
      781 ctacatgcaa atgattttca tctgatggct ccctcttgac cctgccctct ccagtcaccc
      841 attcttccgc ctgggcctgc atcgccctca atgagttccc ggacatgcgc tccagcctaa
      901 aggttttgct tctgcggatt gtggacgagg gctacgcaag gaaaacgtgg cacggaccga
      961 acctgcggca gtccctgcgg ggcgtcaccg cgaagcaggc ggcatggcgc ccgggcttcg
     1021 gccgccataa catctgggag ctgaccctcc atgccgcgta ttggaagtat gccgtgcgcc
     1081 gccggatcga aggaggcaag cgggggttgt tcgcgctcgg gggaagcaat ttctttgccc
     1141 ggccgcagaa gggcggtctc aacgaagccg cctggcgagc ggacaaggaa ctgcttgaga
     1201 aagagcaccg cgcgctgcgt gcggccattg agagggcgtt ccgcaccggc cgggcacaac
     1261 ggttcatgaa ccagatttgc ggcattgcct ttcacgacgt gtaccacgcg ggacagatcc
     1321 ggctcctgcg ccgcttgcag gagaaaagca aatgaactaa agctccgaga ggagactgcg
     1381 cacaaggcgc gagggatcag cgctttcctg gaacagcctg atggcggcga tgcctgcagc
     1441 gcccacggcg aagcattgcc gcgcgtttgc cgcggtgatt ccgccgatgg ccaggaccgg
     1501 gattcgcacg cggcggcaaa cctcagccag ccgctccatc ccctgcggcg ctccgtagat
     1561 ccgcttcgat ggtgtttcaa agaccggtcc gaaaaagacg taggcagcgc cggccagctc
     1621 ggctgcgcga gcagcgtcca gcgagtgggt tgacaccccg acgagcaaac tctccggccc
     1681 aggtccggga cttttccttt cccggagaag ggcaacgact ccggccggag gcagcgacat
     1741 ctcgcccagg tggattccat ccgcgccggc gctcaggacg acgtcaagac gatcgttgac
     1801 tacgatgcga gcggagggcg gacactgcct ccgcgccgcg cgcaccagtg ttgcgagccg
     1861 cgccgccggc aagtccttct cacgaatctg aatccactgc acgcccgcgg cggccacccg
     1921 ttcgatcacg tcgagaagcc ctcgctcgcc ttcggctgcc gccatctgcg gcgcgccggc
     1981 cagggcgcgg agatccgtaa cgtagcagag cagcgggcta ttggcttgag gcacgcgtcg
     2041 cccgaggact ccgccgtatc atcctggcgg ccagcacgat gtccagcaca ccgattccgc
     2101 tcaccgtgcc gcgcacaaaa aagttcagca tcacggggat caacgacgga tagtgcagca
     2161 ggaagtagtt ctgctcccag aaggcggacc acgggagata gagcaggacc gctccgatct
     2221 cgaaacaaat caacacaacc
//
LOCUS       DASPUL010000521         3874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1597540, whole genome shotgun sequence.
ACCESSION   DASPUL010000521 DASPUL010000000
VERSION     DASPUL010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1597540"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VEJ00_12235"
     CDS             <1..736
                     /locus_tag="VEJ00_12235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXX01975.1"
                     /translation="VLRRIRKVSQGSYIYTVMLTAKSQKKDRHTAMEAGADDYLSKPV
                     DPAELRQRIMVGKRILELQQSLRFAATHDFLTNLLNRSEILAALEREFSRSGREGKPT
                     TVILADIDHFKRVNDNLGHAAGDEVLKEVARRLKVDLRPYDMVGRYGGEEFLIILPGC
                     GLDAGARRADAIRCKVAKEPIATGFGAAPTTISMGVTATDSMRDRSVAEFLREADMSL
                     YAAKNNGRNRVEIFSASAKAAGSGQS"
     gene            836..2119
                     /locus_tag="VEJ00_12240"
     CDS             836..2119
                     /locus_tag="VEJ00_12240"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX01976.1"
                     /translation="MPAPSDARAGRAAFTHPAFVLFQIARFMIVAAVEMQAVAVGWQV
                     YEITKRPLDLGFVGLAQFLPGILLFPISGHASDRFERRHVLSTCYAGFGLCFALLLAL
                     TQHGLHSVTAIYAVLVLLGVVRSFNSTASRSILPSLVPEEHFPNAVAWNATIFQTATI
                     LGPTFGGILYAATHGPSLVYGIAMITAIGATATTFRIKPERKVRLREPMTLKTVFAGL
                     HFIWTKKLILGAISLDLFAVLLGGAVALLPVYAREILRTGPWGLGLMRTAPAVGATLM
                     AVLLAHRPLRGKSGITLLWAVAGFGIFTIVFGLSRSLTVSLAALLLLGASDMISVIIR
                     ATLTQLATPDEMRGRVTAVDMIFIGASNELGQFESGVTAQWFGTVPAVVLGGVGTLAV
                     IGLWAWLFPELRQAGDLHEIRSEDLPTSSQASASS"
     gene            complement(2249..>3874)
                     /locus_tag="VEJ00_12245"
     CDS             complement(2249..>3874)
                     /locus_tag="VEJ00_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01977.1"
                     /translation="SDETHIRVLALDSQGNLIAGTDGSGLIYRISPAGEGFVLYSAPK
                     KEITALALDHQGNIFAAGVGEKRPGATPAVTTPMMAMGAGAPVSTAPQAPGITVAPIA
                     SIMQMGAFPFPGGGASGGSDVYRIAPDGSPERVWTSHDDIVYALAFDAHDRLLAGTGN
                     RGHIFAIDGTDEYSDLLKAPISQLTAFAKAPGGGLYAASSNLGKVFVLGPGPEGEGTY
                     ESDVFDAKIFSRWGRAEFRGSGSVDLLARSGNVDNPDRNWSPWRKIDLGKDAETGVPS
                     ARYAQWKAVLRAGNTKPSVESVTLNYLPKNVAPEVEDVSVQVGVRYQPAPKQPATAST
                     TDLSTGASPAIHFDSPVPSTHDRDAIGVKWSAHDDNDDQLAYSIYYRGDGETRWLLLK
                     DDLSDKAYSFDASLLPDGGYVVKVVASDAPSHSPGESLTAEKTSPRFEVDTTPPRIEN
                     LAASLEGAQIHVRFRAEDGFSTIKRAEYSVDASDWKYVDPTGQLSDSKIEDYDFNAPL
                     EAAKDAGSEHVVVVRVYDKYDNMGAAKTVLHGK"
BASE COUNT          667 a         1187 c         1240 g          780 t
ORIGIN      
        1 cgtactgcgg cgcattcgca aagtcagcca gggaagctac atctacaccg tgatgctgac
       61 cgccaagagc cagaagaaag accggcacac ggccatggag gcgggcgccg acgattatct
      121 ttcgaagccg gtcgatccgg cagaactgcg gcagcgcatc atggtgggca agaggattct
      181 ggagctgcag cagtcgctgc gcttcgccgc gacgcacgac tttctgacca atctgctgaa
      241 ccgctcggaa atcctggccg cgctggagcg ggaattctcg cgcagcggac gcgaaggcaa
      301 gcctaccact gtcattctcg ccgacatcga ccacttcaag cgcgtcaatg ataacctcgg
      361 ccatgccgcg ggggacgaag ttctgaagga agttgctcgg agactgaaag ttgatctccg
      421 accctacgac atggtgggac gctacggcgg agaagaattt ctgatcatcc tgccgggctg
      481 cggattggat gccggggcgc ggcgcgccga cgccattcgc tgcaaggtgg ccaaggaacc
      541 gatcgcaacc ggcttcggcg ccgccccgac cacgatcagc atgggggtta cggcgacgga
      601 ttcaatgagg gatcgctctg tagccgagtt tctgcgcgag gcggacatga gtctctatgc
      661 cgccaagaat aacggcagga accgggtcga gattttttcg gcatcggcga aggctgccgg
      721 ttccggccag agttagacgg tcgccaacgc acagttccgc tggcatcttc cagtctgcag
      781 gtctgagaac tgcccttcct gcacttcttc gctttctatg tcacagtaga agtgcatgcc
      841 cgcaccctcc gacgcacgcg ccggacgcgc cgccttcacc catcccgcat tcgtgctgtt
      901 ccagattgcc cgcttcatga tcgtggcggc ggtggagatg caggccgtgg ccgtgggatg
      961 gcaagtctac gagatcacga agcggccgct ggatcttggg ttcgtgggcc tggcgcagtt
     1021 tctgccgggg attctgctgt tcccgatttc cggtcatgcc tcggaccgct tcgagcggcg
     1081 gcatgtgctc tccacctgct acgccgggtt cggcctctgc ttcgccctgc tgctcgctct
     1141 tacacagcac ggcctgcatt cggtgactgc gatctacgcc gtgctggtgt tgctcggcgt
     1201 ggtgcggtcg ttcaacagca cggccagccg ctcgattctg ccctcgctgg tgccggaaga
     1261 gcatttcccg aacgccgtag cctggaatgc cacgatcttt cagacagcca cgattctggg
     1321 gccaacgttc ggcggcattc tttacgccgc gactcacggt ccgtccctgg tgtatggcat
     1381 cgcgatgatc acggccattg gagcgacggc gacgacattc cgcatcaagc cggagcgaaa
     1441 agtccggcta cgcgaaccga tgacgctcaa gactgtattc gcgggattgc acttcatctg
     1501 gaccaagaaa ttgattctgg gggcgatctc gctggatttg ttcgccgtgc tgctgggtgg
     1561 ggcggtcgcg ctgcttcccg tttacgcacg tgagattctg cgcaccgggc cctggggact
     1621 cggcctgatg cgcactgccc cggcggtcgg ggcaacgctg atggcggtgc tgctcgctca
     1681 ccggccgctg cgcggaaagt ccgggatcac attgctctgg gcggttgccg gattcggaat
     1741 cttcacgatt gttttcgggc tttcgcgcag tctcaccgtg tctctggctg cactgctgct
     1801 gctcggggcg agcgacatga tcagtgtgat catccgcgcc acgctcacgc agctggccac
     1861 ccccgacgag atgcgcgggc gcgtcaccgc ggtcgacatg attttcattg gagcttcgaa
     1921 cgaactcggg cagttcgaat ccggcgtgac ggcacagtgg ttcggcacgg taccggcagt
     1981 cgttctggga ggcgttggca ccctggctgt gattggcttg tgggcgtggc tgtttccgga
     2041 actgcgacag gcgggcgatc tgcacgagat tcgcagcgag gatttgccga cttccagtca
     2101 ggcgtccgct tcaagctgac gaaaatcctg gaccgcagag ggcgccaaga aataccgcag
     2161 agaccgctga gaagaacgat tgcggctcgc tgcaatcgcg cctggtcgat cactctcatg
     2221 cggtctccgt cgaggctgtg cgtcggtcct acttgccgtg cagcacggtt ttggccgcgc
     2281 ccatgttgtc gtacttgtcg tagacccgaa ctacgacgac atgctcagat ccagcgtctt
     2341 tcgccgcttc gagcggggcg ttgaagtcgt agtcttcgat ctttgaatcc gagagctggc
     2401 cggtcggatc gacatatttc cagtcgctgg catcgaccga atactcggcg cgcttgatgg
     2461 tagagaagcc atcttcggcg cggaagcgga catggatctg agcgccttcg agggacgccg
     2521 ccaggttctc gatgcgcggg ggggttgtgt ccacttcaaa tcgggggctg gttttctccg
     2581 cggtcaggga ttcgccgggc gagtgcgacg gtgcgtcgga ggccaccact tttaccacgt
     2641 agccgccgtc gggcagcagg ctggcatcga aggaataagc cttgtcgctc aggtcgtcct
     2701 tgagcaacag ccagcgcgtc tctccgtcgc cgcggtagta gatggagtac gcgagctgat
     2761 cgtcgttatc gtcgtgcgcg ctccatttca cgccgatggc atcgcggtcg tgcgtgctgg
     2821 gcaccgggct atcaaaatga atggcgggcg aggcgccggt gctcaagtcc gtggtggacg
     2881 cagtcgccgg ctgttttggc gcaggctggt agcggacccc cacctgcacg gaaacgtcct
     2941 ccacctccgg agccacgttc ttgggaaggt aattcaaggt aacgctctcg accgacggct
     3001 tcgtgttccc cgcccgcaac acggccttcc actgggcgta gcgggcggaa ggaacgcccg
     3061 tctccgcgtc tttgcccaga tcgatctttc tccatggact ccagttgcgg tcgggattgt
     3121 ccacgttgcc gctgcgcgcc agcagatcga cgctgccgga gccgcggaac tcagcgcgtc
     3181 cccagcgcga gaagatcttg gcgtcaaaca cgtcgctttc gtaggtccct tctccctccg
     3241 ggcccgggcc gagaacgaag acctttccga gattgctgct ggcggcgtaa agtccgccgc
     3301 ctggagcctt ggcaaaggcc gtcagttgcg agatcggcgc tttcagcaga tccgaatact
     3361 cgtcggtccc gtcgatcgca aaaatatgcc cgcggtttcc ggttccagcc agcagtcggt
     3421 cgtgcgcatc gaaggccagg gcgtacacga tgtcatcgtg cgaagtccag acgcgctcgg
     3481 gagagccgtc aggagcgatg cggtagacat ccgatccgcc gctcgcgccg ccgccgggga
     3541 aggggaacgc ccccatctgc atgatgctcg cgatgggagc gacggtaata ccgggagcct
     3601 gcggggcagt ggataccggc gcgcccgctc ccatggccat catcggcgtg gtcacggcgg
     3661 gtgtcgcgcc ggggcgtttc tcgccgacgc ccgcagcgaa gatgttgccc tgatgatcca
     3721 gagccaacgc ggtgatttct tttttcggtg cgctgtagag cacaaatccc tcaccggctg
     3781 gagagatccg gtaaatcaga ccgctgccgt cggtgcccgc gatcaggttg ccctgcgaat
     3841 cgagcgccag cacgcgaatg tgggtttcat cgct
//
LOCUS       DASPUL010000522         3920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_931611, whole genome shotgun sequence.
ACCESSION   DASPUL010000522 DASPUL010000000
VERSION     DASPUL010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931611"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VEJ00_12250"
     CDS             complement(<1..247)
                     /locus_tag="VEJ00_12250"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXX01978.1"
                     /translation="MARLFQTRRLAQHRLGSRGQPEESRAAILQAAAHEFAEHGIAGA
                     RTDTIAREARVNKALLYYYFKDKETLYGSVLDSAFSGL"
     gene            433..1419
                     /locus_tag="VEJ00_12255"
     CDS             433..1419
                     /locus_tag="VEJ00_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXX01979.1"
                     /translation="MRSLPVLLAVLTFSLPGLVQAQAAKPPDRQVAVTIDDLPSGTAD
                     RFPAAEITAMTAKLLGTLRDQKIPVVGFVNEKKLYKLGEVDERIKALSMWLDYGFELG
                     NHTFSHPSLNQVPLQVWEDDVIQGEPVIRMLMAQHQMKLRYFRHPFLDTGRDLLTRRQ
                     AEQFLAQRGYRIAPITLDGWDWMFAGLYEDAKKRNDTGLQQQIVKEYLAHHDAEFAYC
                     EEQSRKLLGYEPRQILLLHASNLEADHIGELMEVLRKRGYRFITLQDALEDGAYSLPD
                     TYVGEEGTGWLDHWAITQGRVPPEGPPIAPWVMEKTKELHLSTGAVTAPTEP"
     gene            complement(1533..2522)
                     /locus_tag="VEJ00_12260"
     CDS             complement(1533..2522)
                     /locus_tag="VEJ00_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX01980.1"
                     /translation="MTARLSAVVLLFVGSLVAESGPTARHIRIHVAFASGLCAVSTRV
                     VLLGHSGPVAEGSADEECVVNFDRVPEGKYHLRVSGQNLPDADFGSIAIGRAGPDAFE
                     VRVKGSTDAVGSYGSPASGLVSASDLAVPARARSEFERANELIGKENLKLAIQKLDRA
                     IAIYPLYAAAYNNLGVIYSRLGDLAQARKVLEKAITLDDHFALAYLNLGRMSGASGDF
                     VSAEETLRQAAELDPTEAITWVLLAYSEFMQGRFDEAIATSQKVHQLPGAHAYAHLVA
                     AHAFLKKREAENAIAELQMFLREEPAGSFAERAREELATVEAAVGATVQPRVQ"
     gene            complement(2775..3056)
                     /locus_tag="VEJ00_12265"
     CDS             complement(2775..3056)
                     /locus_tag="VEJ00_12265"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXX01981.1"
                     /translation="MPITVQIPTALRRHTAGVGSLSCTAANLGELFSVLDEKFPELKP
                     HLRDDKGEVRRFLNIYVNEEDIRFLGGPAYAFKEGDEVLLVPSIAGGKN"
     gene            complement(3059..>3920)
                     /gene="thrC"
                     /locus_tag="VEJ00_12270"
     CDS             complement(3059..>3920)
                     /gene="thrC"
                     /locus_tag="VEJ00_12270"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXX01982.1"
                     /translation="AAREGLKAWIFIPSDLEPAKILGTNVFGAKVVRIAGNYDQVNRL
                     CSQIADEHRWGFVNVNLRPYYSEGSKTVGFEIAEQLGWRLPDNVVVPMAGGSLITKIK
                     KAFDELIKLGLVEAREVKFFGAQATGCSPISTAVKTGSDEIEPQKPATIARSLAIGNP
                     ADGHYAIKTIKGSGGWSEDVSDPEVVDSIRLLAETEGIFTETAGGVTVGCAAKLYRQD
                     RILPEETTVLCITGNGLKTTDVLAGAYKTERPIAPKLAEFEQYLQQTLDVAEVPGLIA
                     ASEEAAGVGA"
BASE COUNT          785 a         1220 c         1149 g          766 t
ORIGIN      
        1 tcaatcccga aaaggcgctg tccagaaccg agccgtacag ggtttccttg tccttgaagt
       61 agtaatagag caacgccttg ttgacgcggg cctcgcgggc gatcgtgtca gtgcgggcgc
      121 cagcgatgcc gtgctcggca aattcatgcg cggcagcctg cagaatggcg gcacggcttt
      181 cctcgggctg gccgcgcgag cccaggcggt gctgcgctag acgacgagtt tggaaaagcc
      241 gggccatgga atgcacccct gtcttaatta accagttagt tagattatag cacgcatctg
      301 cccgatgcaa aaatcctccc ggcggcgctc attttccaca gaatggaatt ccctctactc
      361 gttctcctcg gcccctcacc tcccgtgttc taatacccct tctactggaa taccttcggc
      421 gagggcttgc tcatgagatc gttgcccgtc ctgctcgctg tgctgacctt ttcgctcccc
      481 ggtctggtgc aggctcaggc cgcaaaaccg cccgatcgcc aggtcgctgt caccatcgac
      541 gaccttccct ccggcacggc tgaccgcttc ccggcggcag aaatcaccgc gatgaccgcc
      601 aaactgctgg gtacgctgcg cgatcagaaa atccccgttg tcggcttcgt caacgagaag
      661 aagctctaca aactgggcga ggtcgacgag cgcatcaagg ctctctcgat gtggctcgac
      721 tacggcttcg aactgggcaa ccacacgttc agccatccgt cgctgaacca ggtgccgctg
      781 caggtgtggg aagacgacgt gatccagggt gagcccgtca tccgcatgct gatggcgcag
      841 caccagatga agctgcgcta cttccgccat ccttttctcg acacgggccg cgacctgctc
      901 acgcgccgcc aggcggagca gttcctcgcc cagcgcggat accgcatcgc gccgattacc
      961 ctggacggct gggactggat gttcgccggc ctgtatgaag acgccaagaa gcgcaatgac
     1021 acggggctgc agcagcagat tgtgaaggaa tacctggcgc atcacgacgc cgagttcgcc
     1081 tactgcgagg agcaatcgag aaaactcctc ggctacgaac cccggcagat tctgctgctg
     1141 catgccagca atctggaggc ggaccacatc ggggagttga tggaggttct gcgcaaacgc
     1201 ggctatcgct tcatcacgct gcaggacgcg ctcgaggatg gggcgtacag cttgcctgat
     1261 acctacgtcg gagaagaagg cacgggctgg ctcgatcact gggcgataac ccagggaagg
     1321 gttccgcctg agggaccacc catcgcgcct tgggtcatgg agaagacgaa agaactgcac
     1381 ctgtcgaccg gcgcggtgac ggcgccgact gagccgtagg tgatccgagg cggagtcgag
     1441 catcgggagc gtgagcggcg agcagacagc gacacgcctg aagcgaaaag cgggtgacct
     1501 cgatagtgcg gggtcggtgg agaaaccggt tcttactgaa cacgtggctg caccgttgcg
     1561 ccgacggctg cttcgacggt agccagttcc tcgcgcgctc tttctgcaaa ggaacccgca
     1621 ggctcttccc tgaggaacat ctgcaactcg gcgatggcgt tttcagcctc tcgcttcttg
     1681 agaaacgcat gtgccgccac aagatgggcg taggcatgag cgccgggcag ttgatgcact
     1741 ttctggctgg ttgcgattgc ctcgtcgaag cgcccctgca tgaactcaga gtaggccagc
     1801 agcacccagg ttatcgcctc agtcgggtca agttcagcgg cttggcgcaa tgtctcttct
     1861 gcggatacaa aatcgccgga tgctccgctc atcctcccca gattgaggta ggccagggcg
     1921 aagtgatcgt ccagggtgat ggctttctcc agcaccttac gcgcttgcgc caaatcaccg
     1981 agacgagaat agattacgcc cagattgttg taggcggccg catacaacgg atagatcgca
     2041 atcgccctgt ccagcttctg gatggccagt ttcaggttct cttttccaat cagctcattt
     2101 gccctctcga attcgcttcg agcccgggcg ggcacggcta gatccgaagc cgagaccaga
     2161 ccgctggccg gcgaaccata cgagcccacc gcatcggtcg accctttcac tctgacctca
     2221 aaagcatccg gaccggccct accgatcgca atgctgccaa aatcagcgtc gggcaggttc
     2281 tgcccggaaa cgcgcagatg gtactttccc tccggcacgc ggtcaaagtt aacgacgcac
     2341 tcctcatcgg cgcttccctc agctaccggg ccagagtggc cgagcagaac cacacgcgtg
     2401 gagacagcac agagtccact ggcaaacgcg acgtgaatgc gaatatgccg ggctgtcggg
     2461 cccgactccg ccaccagcga ccccacgaag agcagaacta ctgcggacaa gcgagcggtc
     2521 atggcagcct ccggaactga cgctgcaatc cactcgtccg cctgtcaccg gaaggaagcg
     2581 aaggtcgcgc gccccaactt ggcgatgaga cgcgtgaaaa aatgaacgga tagctgccgg
     2641 ttgctgtgga agaagagatt ttctggagct gagtcgccgt tagaaactag acacgcacac
     2701 aacacattgc aacacaactg agtgtgttga atttgttgtg aatgttgcgt cgcggagaac
     2761 cgcggacttc gcagtcaatt ctttccgccg gcgatcgagg gcacgagcag gacttcgtcg
     2821 ccttccttga aagcgtacgc aggtccgccg aggaaacgaa tgtcttcctc attcacatag
     2881 atattcagaa aacgccgaac ctcgcctttg tcatcgcgca ggtgcggttt gagttcgggg
     2941 aacttctcgt cgagcaccga gaacagctcg cccaggttgg cggcagtgca ggagagcgag
     3001 ccgacgcccg cggtgtgccg ccgcaaagcg gtcggaattt gtaccgtgat cggcatgact
     3061 aggctcccac gccggcggct tcttccgaag ccgcgatcag ccccggcacc tcggcgacgt
     3121 ccagcgtctg ctgcagatat tgttcgaact ccgccaactt gggcgcgatc ggccgctccg
     3181 tcttgtatgc gccggccagc acgtcggttg tcttcaatcc attgccggtg atgcacagca
     3241 cggtcgtctc ctcgggcaga atgcggtcct gccgatacag cttggccgcg cagccgacgg
     3301 ttacgccgcc ggccgtttcg gtgaagatgc cttccgtttc cgccagcagc cggatcgaat
     3361 cgactacttc gggatcgctg acgtcctcgc tccatccccc gctgcccttg atggtcttga
     3421 tggcatagtg cccgtctgcc ggattgccga tcgcgagcga acgcgcaatc gtcgccggct
     3481 tctgcggctc aatctcatcg ctgccagtct tgaccgcggt tgagatcggc gagcagccgg
     3541 ttgcctgtgc tccaaaaaac ttcacttccc tagcttccac caggccgagc ttgatcaatt
     3601 cgtcgaatgc tttcttgatt ttggtgatga gcgatccgcc ggccatggga acgacgacgt
     3661 tgtccggcag ccgccagccc agttgctcgg cgatttcgaa gcccacggtc ttcgatccct
     3721 cggagtaata gggccgcagg ttcacattga cgaatcccca acgatgctcg tcggcgattt
     3781 gcgagcacag ccggttgacc tgatcgtagt tgccggcgat gcgtacaact ttcgcgccga
     3841 agacgttcgt gcccagaatc ttggctggct cgagatcgga gggaatgaaa atccacgcct
     3901 tcagcccttc gcgcgccgcg
//
LOCUS       DASPUL010000523         6153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_931819, whole genome shotgun sequence.
ACCESSION   DASPUL010000523 DASPUL010000000
VERSION     DASPUL010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6153)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6153
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931819"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VEJ00_12275"
     CDS             <1..627
                     /locus_tag="VEJ00_12275"
                     /inference="COORDINATES: protein motif:HMM:NF004005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXX01983.1"
                     /translation="VIAGYGWCGRGLASRARGAGAEVIITEVDPTKALEAVMDGFRVM
                     SMTEAAKLGDVFCTVTGNKSVLTKEHFALMKDGAIISNSGHFNVEIDIPSLEAMSSSK
                     RTTRNFVDEYSLKDGRKINLLGEGRLINLAAAEGHPASVMDMSFADQSLSVEYMVKNA
                     KKLERRVYRVPDELDKRVARLKLESMGIKIDRLTPEQEEYLAGWSEGT"
     gene            complement(956..1426)
                     /locus_tag="VEJ00_12280"
     CDS             complement(956..1426)
                     /locus_tag="VEJ00_12280"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXX01984.1"
                     /translation="MKPTRAISPPGTYFVTFSTFQRATLFVVERYARPFLKTLYGYRR
                     QGRFQLHAFVLMPDHVHLLLTPAPELPPERAVQLIKGGYSHAVGVEISRREIWQKGFT
                     DHRIREAQDFAGHRLYIHKNPVVGDWLRTRASIGMARRFRDSGWIRGPQRLKPR"
     gene            complement(1705..2037)
                     /locus_tag="VEJ00_12285"
     CDS             complement(1705..2037)
                     /locus_tag="VEJ00_12285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01985.1"
                     /translation="MADDSVTISPGPPFRAAMALAIVADALQLVIFPVFVAGAMSPPD
                     DLLDLGIAAVMVHLLGWHWEFLPSFLAKLVPGVDLVPFWTLAVANVWRKAKAVAAASV
                     DKPALPER"
     gene            complement(2313..2690)
                     /locus_tag="VEJ00_12290"
     CDS             complement(2313..2690)
                     /locus_tag="VEJ00_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01986.1"
                     /translation="MRVSSALWGAALCLCLAPMLSAQDAVKVDPKHYSVISENDQVRI
                     LKVHYGPHEKSVMHSHPASVAVFLNDGKGTFTFADGKKQDFTAKAGDAQYEAATTHLP
                     EAGDGPIDVIVVELKGHAKSEMK"
     gene            2900..3523
                     /locus_tag="VEJ00_12295"
     CDS             2900..3523
                     /locus_tag="VEJ00_12295"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXX01987.1"
                     /translation="MPRQPDPDVEGRILDVAHKLWKHGGDKALTMRAVARAARTNTPA
                     VYRRFKDKKDIQRALLLRIRQDVIAEMEKAKTAAEACERYLDFALNHPYEYELFYRHD
                     HELLHKRGHDGVHDKLVNRPGVDAMRSILAEGLGGAPEDHARLALALWMVGHGAAMLM
                     IAKMIAPESQGAARSVFRDTVQMLLREASSMSEASTKVADGGRTARG"
     gene            3606..3980
                     /locus_tag="VEJ00_12300"
     CDS             3606..3980
                     /locus_tag="VEJ00_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXX01988.1"
                     /translation="MKLLLVEDSKFLRMSTERALVAAGYQVVSAGDGEEALRLARQQA
                     PQLILLDIMLPKVSGPDVLRALKKDAATAAIPVMMLTGLSQKNAKALEKDGAAAFFEK
                     SDAMLTKGPGSLVEAIGKVLKK"
     gene            4152..4565
                     /locus_tag="VEJ00_12305"
     CDS             4152..4565
                     /locus_tag="VEJ00_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXX01989.1"
                     /translation="MTKILLVDDSKFLRLATERALARAGYDVTTATDGETALRLARER
                     TPDLILLDMLLPKVPGPEVLKALKKDPVTAGIAVVVFTGLSHKNAARLQHDGAFAFLE
                     KSELGLEKGSEALLAALAEIVRKLNLEVPRAAVAK"
     gene            4851..5588
                     /locus_tag="VEJ00_12310"
     CDS             4851..5588
                     /locus_tag="VEJ00_12310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXX01990.1"
                     /translation="MAGATTLGDLASAIGARSQEEAAIVAELKAGSEAAYAWLIGEFQ
                     QPVYGLVYRIVNDPADAADTTQDVFLKVFRGMKHFHGSSSLKTWIYRIALHEAANRRR
                     WWFRHKARETSIEPVESDAGIGSEAMQAALTDQGDSPFDNVAHREVQRRVEEEVQKLP
                     EPYRTTLILRDLEDMSYEEIAEVLEISLGTVKSRLTRGRETLRQKLSEYVREVGNELG
                     LAEPEEKTEGGLRPQVAGGGRRAEVMP"
     gene            5585..>6153
                     /locus_tag="VEJ00_12315"
     CDS             5585..>6153
                     /locus_tag="VEJ00_12315"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXX01991.1"
                     /translation="MRRAETAGTEKIAGLRCSEARHLLSPYLDGAVTGSEMLALQRHL
                     SDCAACGEQYQSLRRTQQLIMSMPRPKAPADLGLRLRVAISREAAQARRPYEGLRVRL
                     ENTVRAFMVPATAGLLSALLIFGVAMAYFVVPASLQADNDVPLTMVNTAPQLEQSAFG
                     MTLDTINTDSLVIEAYIDSNGRVQDYQILS"
BASE COUNT         1216 a         1733 c         2099 g         1105 t
ORIGIN      
        1 gtgattgccg gctacggatg gtgcggccgc ggcctcgcca gccgcgcccg gggcgccggc
       61 gccgaagtca tcatcaccga agtcgatccc accaaggcgc tcgaagccgt gatggacgga
      121 ttccgcgtca tgtcgatgac cgaggccgcc aagctcggcg acgtcttctg caccgtcacc
      181 ggcaacaaga gcgtgctcac caaagaacat ttcgcgctga tgaaggacgg agccatcatc
      241 tccaactccg gacacttcaa cgtcgaaatc gacattccgt ccctcgaagc catgtcgtcg
      301 tcgaagcgga ctacgcgcaa cttcgtcgat gagtactcgc tcaaagacgg acgcaagatc
      361 aacctgctcg gcgaaggccg cctgatcaat ctcgcagcag ccgaaggcca ccccgccagc
      421 gtcatggaca tgagcttcgc cgaccagtcg ctctcggtcg agtacatggt gaagaacgcg
      481 aagaagctgg agcgccgggt ctaccgcgtc cccgacgaac tcgacaagcg cgtcgcccgc
      541 ctgaagctcg aatccatggg catcaagatc gaccggctca cccccgagca ggaagagtac
      601 ttggcggggt ggtcggaagg aacgtaagag cgggcgtcgg gcttccggcg tccggcttca
      661 gcaaaaccaa gtgaaattgg gtgccccact tctggccgcc tttgccagaa gtggggcttt
      721 tcgtttggag aagcaagaac catgtagggg cggccgccct cgtccccccg gcagcccgga
      781 agttccgggc cgctgcgctt ttatgtggcg cgggcgccct cgcccgcggc ttttgatgtt
      841 ggtggaagaa cggcgcttca gcgccgcgta aagcggaatc acaacgaaac cggttctgac
      901 ctgcgggcac gaacgggaag ggtgcgactt cagtcgcacc gttgggcgcc cgcacctacc
      961 gcggcttcag ccgctgaggg ccacggatcc agcctgaatc ccggaaacgc cgagccatac
     1021 cgatactcgc tctcgttcgc aaccagtctc ccacaaccgg attcttgtgg atgtagagac
     1081 ggtggccggc gaaatcctga gcctcgcgga tgcggtggtc cgtgaatccc ttctgccaga
     1141 tttcgcgccg gctgatctct acccccacgg cgtgggaata accgcctttg atcagttgca
     1201 ccgcgcgttc cgggggcagc tcgggagcag gtgtcaggag caggtggacg tggtcgggca
     1261 tgagcacaaa ggcgtggagc tggaaccggc cctgacggcg gtatccgtaa agagttttca
     1321 ggaacggcct tgcgtatcgt tcgactacga agagcgttgc tcgctgaaag gtagagaagg
     1381 tcacgaagta cgtgccgggc ggggaaatgg ctcgtgtcgg tttcatcggt gccggccctc
     1441 agcggctaaa gccgggcctc ttgcccaggc taatgcggta cgaccgaagt cgtacccttc
     1501 ccggccggga atattgccgc agagtaccga ttggggaatg gggaggacga accgcagagg
     1561 ctgccgctcc tttcgcagtt ttcgaagggt gggagtcgcg gaggtactcg gagcggcccg
     1621 atacttccgg gccgccggac agccgagggc ggctgtcccc acatgaccgc ggatgaggat
     1681 ggcaggactt cggagtgaag gctgctagcg ttcgggcagt gccggcttat cgacagaggc
     1741 ggccgccacg gctttggctt tgcgccagac gttggccacg gccagcgtcc agaaggggac
     1801 caggtcgacg cccgggacga gcttggccag aaacgacggc aggaactccc agtgccagcc
     1861 caacagatgc accatcacgg cggcgatgcc gaggtcgagc aggtcatcag gcggagacat
     1921 cgcccccgcc acgaagactg ggaagatgac gagttgcagc gcgtcggcaa cgatggccag
     1981 cgccatcgcc gcgcggaacg gcgggccggg cgagatggtg acggagtcgt cggccatggc
     2041 gatgcctcct gcctcaattc tagacgaggc ggcacccttt gggtccgatg atcggcagca
     2101 cgatgcgcct gggccgccgg gcggacgagg gcgtccgccc ctcatgtgaa ttccccactt
     2161 ctcgcgcaca gaacgccgcg agaaatgggg caccccgcga aaatgcaggt tcgtcccctt
     2221 cggctgcgct cagcgtcgga atgacaaacg gcgtggagaa gccgttatcg cggcggctat
     2281 ttcgtccccg acagagcgtc tgacttcatg gcctacttca tttccgattt ggcgtggcct
     2341 ttcagctcca ccacgatcac gtcaattgga ccgtcgcctg cctcgggcag atgggtcgtc
     2401 gcggcctcgt actgcgcgtc gcccgccttg gctgtgaagt cctgcttctt gccgtcagca
     2461 aatgtaaacg tacccttgcc gtcattcagg aagacggcca cgctggcggg atggctatgc
     2521 atcaccgact tctcgtgcgg tccatagtgc actttcagaa tgcgtacctg atcgttctct
     2581 gatatcaccg agtagtgctt gggatcgacc ttgacggcgt cctgagctga aagcatgggg
     2641 gcgagacaga ggcacagggc cgcgccccat agggctgatg acactcgcat atcgctctcc
     2701 ttgaatgtta tgtttcgaaa agcggcccta ccctacgccg ccgggcgggc gatgtcaaat
     2761 ttgaagatgg gaagcgcacg tttgagaact gacaccacgg gtcgcggctt tcgccgcggg
     2821 cttgtgtcag ggggcggtgt tatgcataca ttgttaacgg aacgcgagcg gtaaacggcg
     2881 ttcccgggag gaacgaacga tgccccggca gccggacccc gatgtggagg gccgaatcct
     2941 tgacgttgcg cacaagctgt ggaagcacgg gggcgacaag gctctcacca tgcgggcggt
     3001 ggcgcgggcg gcgcggacga acacgcccgc cgtctaccgg cgcttcaagg acaagaagga
     3061 tatccagcgg gctctgctgc tgcgcatccg gcaggatgtg atcgcggaga tggagaaggc
     3121 gaagactgcg gcggaggcgt gcgagcgtta tctggatttt gctttgaacc atccgtacga
     3181 atacgaactc ttctaccggc atgaccatga attgctgcac aagcgcggcc acgatggagt
     3241 gcacgacaag ctggtgaacc ggcctggcgt cgatgccatg cggagcatcc tggccgaagg
     3301 gctgggcgga gccccggagg atcacgcgcg gctggcgctg gcgctgtgga tggtggggca
     3361 cggggcggcg atgctgatga tcgcgaagat gattgcgccg gagtcgcagg gtgcggcgcg
     3421 gtcggtgttc cgcgacacgg tacagatgct gctgcgggaa gcctcgagta tgtcggaagc
     3481 gagcacgaag gtggcagacg gcggtcgcac cgcgcgcggc tagctcggac cataagagca
     3541 tccgagcggc gagggcgtgg cggcgtagtg tgggacagga agcgattgga ggcgcaacgg
     3601 tggcgatgaa gttgttgctg gttgaggaca gcaagtttct tcgcatgtcg acggagcggg
     3661 cattagtcgc ggccggatac caagtcgtgt cggccggcga cggagaagaa gctttgcgac
     3721 tggctcgtca acaggcgccg caactgattc tgctcgacat catgttgccg aaggtcagcg
     3781 gaccggacgt gctgcgcgcg ctgaagaaag atgcggcaac ggcggcgatc ccggtgatga
     3841 tgctgaccgg gctgtcccag aaaaatgcga aggcgctgga gaaagatggg gctgccgcgt
     3901 tctttgagaa gtccgacgcg atgctgacga agggccccgg ttcgctcgtc gaggccatcg
     3961 ggaaagtact caagaagtga gctttggcgg gactcgcgtt cgcaggctga ccgactgaac
     4021 ctgagaagat tctcatgcat tgatcctttc ccctgttggt ttccccccac cgcgccgata
     4081 ggagatttgt accctgcaga atcggcccag ccatgtcgca catccggcgc ggagttctgc
     4141 aggctcactg catgacaaag atcctgctgg tcgacgacag caagttcctg aggctggcca
     4201 cggagcgagc gctggcccgg gccggctatg acgtgaccac ggccaccgac ggggaaactg
     4261 cgctgcgcct ggcccgggaa agaacgcctg atctgatcct gctcgacatg ctgctgccga
     4321 aggtgccggg gcctgaagtg ctgaaggcgc tgaagaagga tcctgtgacg gcgggcattg
     4381 cggtagtggt gtttaccggg ctatcgcaca agaatgccgc ccgcctgcaa cacgatgggg
     4441 ccttcgcctt cctggaaaag tcggaactgg gactggagaa ggggagcgag gcgctgctcg
     4501 cggcgttggc ggagattgta cggaaattga atctggaagt gcctcgggca gctgtggcga
     4561 agtgaggtcg ctgactctgc agagtagggg cgcgatgacg ttcgctggta acttctgccg
     4621 gtgaccgttt ggttcctgat tgttccttta gtcccggccc acccacgttt agtcccagct
     4681 tgacctcctt acccgctaag aacaaagcac ttagagtcgg gaacctggcg cggcccggcg
     4741 gagtctatct ttcatgagtg cgtatcccga aaagcagttg gccggatgtg gtggtgcagt
     4801 aattttcgcc ggggtgaggt ggaaaatcct ataatcaaag gacgccgtat atggcgggag
     4861 caaccacctt gggggatctg gcaagtgcaa ttggagcgcg gtcgcaggaa gaagctgcga
     4921 tcgtcgccga gctgaaggct ggctcggaag cggcgtacgc ctggctgatc ggtgagttcc
     4981 agcagccggt gtacgggcta gtgtaccgca ttgtgaatga tccggccgac gcggccgaca
     5041 ccacgcaaga cgtatttctg aaggtctttc gcggtatgaa gcacttccac ggctcgtcca
     5101 gcctgaagac ctggatttat cgcatcgcgc tacatgaggc ggcgaaccgg agacggtggt
     5161 ggtttcggca caaggcacgc gagacgtcga tcgagccggt ggaatcggac gcagggatcg
     5221 gcagcgaggc aatgcaggcg gcgctgactg accaagggga ttctcccttc gacaatgtgg
     5281 cgcatcgcga ggtgcagcgc agggtggaag aggaagtgca gaaacttccg gaaccgtatc
     5341 gcacaacgct gatcctgcgc gatctggagg atatgtcgta cgaggagatt gccgaggtgc
     5401 tcgagatttc gctggggaca gtgaaatcac ggctgacgcg aggcagagag actttgcggc
     5461 agaaactgtc ggaatatgtg cgtgaagttg gaaacgagct ggggttggcc gagcctgagg
     5521 aaaaaacaga agggggtttg cggccacagg ttgctggcgg gggtagaagg gctgaggtga
     5581 tgccatgagg cgcgcggaaa cagcgggcac ggagaaaatc gcgggattgc ggtgctccga
     5641 ggcgaggcat ctgttgtcgc catatctgga tggcgcagtg acgggctcgg agatgctcgc
     5701 gctgcaaagg catttgtcgg actgcgcagc ttgcggcgag cagtatcagt ccctgcggcg
     5761 gacgcagcag ctgatcatga gcatgccacg tccgaaagcg ccggcagatc tgggattgag
     5821 gctgcgggtg gcgatttcgc gggaggcggc gcaggcacgg cggccttacg aaggtctgcg
     5881 cgtgcgcctg gagaatacgg tgcgcgcgtt catggttccg gccactgcag gcttgctgag
     5941 cgcgctgctg atttttggag tggcgatggc gtatttcgtc gtgccggcct cgctgcaggc
     6001 ggacaatgat gtgccgctga ccatggtcaa cacggcgccg cagctggagc agagcgcgtt
     6061 cggcatgacg ctggacacca tcaacacgga ctcgctggtg atcgaagcct acatcgactc
     6121 caacgggcgg gtgcaggatt accagattct ttc
//
LOCUS       DASPUL010000524         4807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_506845, whole genome shotgun sequence.
ACCESSION   DASPUL010000524 DASPUL010000000
VERSION     DASPUL010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506845"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1367
                     /locus_tag="VEJ00_12320"
     CDS             84..1367
                     /locus_tag="VEJ00_12320"
                     /inference="COORDINATES: protein motif:HMM:NF023431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HXX01992.1"
                     /translation="MAHTLHHHAHAHPHPDSQFPTRRAFLSSLSSLISAAVLAPWASG
                     QDHAPTPGELAERARRMSEDYELKGLAEPFRGITTDGNVLPGLFEIRPSGVSTEPIRN
                     AAERFIATLTTVQLIRTMFPIEDAQWRKWMNQHFYPRQGISLWEMNDAQREAAYGLMQ
                     ASLSARGFELVRNIMRLNETLAELADPDERPLLGEWLYYITILGKPSATEPWGWQFQG
                     HHAIINCFVLGDQVVMTPLFVGSEPVRAPSGKYKGVTVLQQEQNDGLAMLLALPDAQR
                     KQAVLAFSKTGNNNLTEAFKDNVVIDYSGLRANALADLGRQRLRDLIHLYVSNMDDGH
                     TRVKMDEVDRHLDDTWFSWIGGTQPDSVFYYRVQSPVILIEFDHQRPANLSRLAANPE
                     LPTQQHIHCVVRTPNGNDYGKDLLRQHYLAHPHTT"
     gene            complement(1427..2467)
                     /locus_tag="VEJ00_12325"
     CDS             complement(1427..2467)
                     /locus_tag="VEJ00_12325"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HXX01993.1"
                     /translation="MHADVLLVLITILVALIFDFVNGFHDSANSIATVVSTRVLSPRL
                     AVLWAATFNFLAAFFLGTAVAQTIGSGMIRLEFVDQYVVLTGLLGAILWDLFTWWWGL
                     PTSSSHALIGGYAGAAVANAALRHGTNSIVAVIIPHGWYKTLIFIVVAPIIGLILAFI
                     FIVSAHWIFRKSAPQPVDRLFRRLQLLSAAAYSLGHGANDAQKTMGIIAGALFTGGFM
                     SRSDMLEGWGRLHWPIILAAHAAIALGTYLGGWRIVKTMGQKITKLKPMGGCCAETAG
                     AITLFATALVGIPVSTTHTITGAIVGVGAVHRLSAVRWGIAGRIVWAWVFTIPASALM
                     AAAALVIVNRFR"
     gene            complement(2514..3125)
                     /locus_tag="VEJ00_12330"
     CDS             complement(2514..3125)
                     /locus_tag="VEJ00_12330"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HXX01994.1"
                     /translation="MRLIPHEPRFFELFAELSSYLTEGANLLRNILEDPRDLDMKVEQ
                     VQAIEHKGDRATHAIITKLNQTFITPFDREDIHRLASSLDDVLDFTNLAATRLAMYRI
                     TQPPPAAAELAGLIVLQSEELARGVSVLEKNGDVTKYCEEINRLEDEADHVGRRAIAH
                     LFESEKDPIQLIKLKELYEVLEVATDKAEDAANVLETIFLKNA"
     gene            3577..4179
                     /locus_tag="VEJ00_12335"
     CDS             3577..4179
                     /locus_tag="VEJ00_12335"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX01995.1"
                     /translation="MRRLLSTWCLLALLVTGGAAASAQINPYKEGTPGVTGYRSEVLS
                     EVIIQEDKFTRLAEAIPADKYTWRPSPDARSFAEVFLHVSAANFNLYKLVGTPPPAGF
                     DVRGLEHSTTDKAKVISILKDSFAHAKDAIKAMPDADLEKSMEWFGGRNTERGILLFI
                     VRHSAEHLGQSIAYARSIGVVPPWTEDLKKQADKPAPAKK"
     gene            4332..>4807
                     /locus_tag="VEJ00_12340"
     CDS             4332..>4807
                     /locus_tag="VEJ00_12340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXX01996.1"
                     /translation="MDPAHTISTQGLTRRFGDLVAVEGVNLRVAPGQFFGFLGPNGAG
                     KSTTIKMLTGLLAPSSGRIEILGLDLTTHPVEVKRQIGVVPEGMALFGRLTGAEFLSF
                     AGRMYGLDRQTAAQRATELLEFMQLADQPKKLVTDYSHGMQKKLAMAAAVIHGPKVL"
BASE COUNT         1005 a         1522 c         1364 g          916 t
ORIGIN      
        1 gtgcgacgtt ctgccaacgg ctgatctccc cattgcgggc ccgtactccc cgtgctatcg
       61 taccgcgacg tcgcggaggg aacgtggcac acacgctgca tcatcacgct cacgcccacc
      121 cgcaccccga ctcacaattc cccacgcgcc gcgcctttct ttcctcgctt tcctcgctca
      181 tctccgcggc cgtccttgcc ccatgggcct ccggccagga tcacgctccg accccaggcg
      241 agttggcaga acgcgctcgg cggatgtccg aagactacga actgaaaggg ctggccgaac
      301 cctttcgtgg gattaccacc gacggcaacg tcctgcccgg actgtttgaa atccgaccga
      361 gcggagtttc gaccgagccc atccgcaacg ctgccgaacg atttatcgcg accctgacta
      421 ccgtacagct catccggacg atgttcccga tcgaggatgc ccaatggcga aagtggatga
      481 accagcactt ctatccgcgc cagggcatca gtttgtggga aatgaacgat gcgcagcgag
      541 aagcagcgta cggcctgatg caagcctctc ttagcgccag aggatttgag ttggtccgga
      601 atatcatgcg cctgaacgaa acgctggcgg aactggccga cccggacgag cgtcctctgc
      661 tcggcgaatg gctgtactac atcaccatcc tcggcaagcc gtcggccacc gaaccgtggg
      721 gctggcagtt ccaggggcat cacgccatca tcaactgttt cgtcctgggc gatcaggtcg
      781 tcatgacccc gctgtttgtc ggatcagagc cggtgcgcgc gccttccggc aagtacaagg
      841 gagtgaccgt actccagcag gagcagaacg acggcctggc tatgctgctg gcgcttcccg
      901 atgcccagcg caagcaggcg gtgctcgcgt tctcgaagac cggcaacaat aacttgaccg
      961 aggccttcaa ggacaacgtc gttatcgact actcgggcct gcgggcaaac gctctggcgg
     1021 atttaggccg tcagcggctg cgcgacctga tccatcttta cgtcagcaac atggacgacg
     1081 ggcatactcg cgtgaagatg gacgaagtcg accgccacct cgacgacacc tggttcagtt
     1141 ggatcggcgg cacccagccc gacagtgtct tttactaccg tgttcagagt ccggtaattc
     1201 tgatcgagtt tgaccatcag cggcccgcca atctctcgag attagctgcg aatcccgagt
     1261 tgcccacgca gcagcacatt cactgcgtcg tgcgcacgcc caacggaaac gactacggga
     1321 aggacttatt gcgtcagcac tacctggcgc acccgcacac gacgtgagcc gtgcgaccca
     1381 cggattcatt tccgggttcc gctctgcgcc ctttctctct tgcgcatcaa cgaaacctgt
     1441 ttacgatgac gagggctgcc gcggccatca aagctgacgc cggaatggtg aacacccagg
     1501 cccatacaat gcggccagca atcccccagc gcacggcgga caagcgatgg acagcaccca
     1561 ctcccacgat cgcgccggtg atcgtatggg tagtgctcac cggaatgccg acgagcgcgg
     1621 tagcgaacag tgtgatcgca cccgccgttt ccgcgcagca tccacccatg ggtttgagtt
     1681 tggtgatctt ctgacccatg gtttttacga tgcgccaacc gccaagatac gttcccaacg
     1741 caatcgccgc gtgcgccgcc agaatgatag gccagtgaag gcgtccccag ccttccagca
     1801 tatcggacct gctcataaag ccaccggtga acagcgcccc ggcgatgatg cccatggtct
     1861 tctgggcgtc attggcgcca tgtcccaggc tgtaggcagc cgccgacaac aactgcagcc
     1921 tgcggaagag gcggtcgacc ggctgcggcg cggacttgcg gaaaatccag tgcgcggaaa
     1981 ctatgaagat aaaggcaaga atcagtccga tgatcggcgc tacgacgatg aagatcagcg
     2041 tcttatacca gccgtgagga atgatgacgg caacgatgga gttggttccg tgtcgcaacg
     2101 ccgcgtttgc caccgcggcc ccggcgtaac cgccaatcag ggcatgagat gaggaagtgg
     2161 gcaggcccca ccaccaggtg aagagatccc agaggatggc gcccagcagc cctgtgagca
     2221 ccacatactg gtcgacaaac tcaaggcgga tcatgccgct gccaatggtc tgggcgaccg
     2281 cggttcccag aaagaaggca gccagaaaat tgaaagtggc ggcccacaac accgcgagcc
     2341 ttggcgaaag gacacgtgtc gacacaaccg tagcgatact gtttgccgag tcgtgaaagc
     2401 cgttcacgaa atcgaaaata agagctacga gtatcgtaat cagaacgagc aggacatcag
     2461 cgtgcaccgg ataaaaggcc ctccaaattg cgcgctaacc gcgggagcgc accttaggca
     2521 tttttcagga agatggtttc gagaacattg gcggcatcct cggccttgtc tgtcgccact
     2581 tccagcacct cgtagagttc cttgagcttg atcagctgga tgggatcttt ctcgctctca
     2641 aacaaatggg cgattgctct ccgcccgacg tgatcggcct cgtcctccaa acggtttatc
     2701 tcttcgcagt acttcgtgac gtcgccgttt ttctccaata cggatactcc gcgggccagt
     2761 tcctcgctct gcagaacaat cagacccgcc agttcagccg cggccggggg aggctgagta
     2821 attctgtaca ttgcgaggcg ggttgccgcc aggttggtga agtccaggac atcatcgagc
     2881 gaggacgcca gccggtgaat gtcttcgcgg tcaaacggag tgataaaggt ctggttcagt
     2941 ttggtgatga tagcgtgagt ggcccgatcc cccttgtgtt cgatggcttg aacctgctcc
     3001 accttcatgt cgagatcgcg tggatcttcc aggatgttgc gcagcaggtt ggcaccctcc
     3061 gtcagataac tggagagttc ggcgaaaagc tcgaagaacc ttggttcgtg tggaatgaga
     3121 cgcatggctc actacccagc tgcgcaggct tctgcgtaat ctccttcagg acgcctggaa
     3181 cacgtgacgc tcgaccaatc gattcaggcg cgtccgagct ggtggagact gctgaagctg
     3241 ctctgtgttg cgtgcaggat ggtcgtctca gtcttgcaat tctggcccta agtccttccg
     3301 aatgcctctc cagaaacgcc gactgagaag cctccgtcca ggcatactaa accggcggca
     3361 acgggtactc ctcaaagaac cacagagtat accgcatacg cgcaggagac ctcaggcacg
     3421 ggcagtgctc gtggcacaac ccatctcgcc catcaggagt cccttgggga catgtgtccg
     3481 tagtcgcctg aacgagcttt cgtcccacga ttcggaaacc cgctctcgaa gaacgtcgcc
     3541 ggcttgctac actacccgga cctgcaagga gatcctatgc gacgcttgct ctctacgtgg
     3601 tgtttactgg ctctgctcgt gaccggtggg gccgcggctt cggcgcaaat caacccctac
     3661 aaggaaggca cgcccggcgt caccggatac cgttcggaag tcctgtccga agtcatcatt
     3721 caggaagaca agttcacgcg tctggccgaa gccattcccg ccgacaaata tacctggcgc
     3781 cccagccccg acgcgcgttc gttcgccgag gtctttcttc atgtcagcgc cgccaatttc
     3841 aacttgtaca aactcgtggg cacgccgccg cccgccggct tcgacgtcag aggcctcgaa
     3901 cactccacca ccgacaaggc gaaggtgatc tccattctca aagactcctt tgctcacgcg
     3961 aaggatgcga tcaaagccat gcccgacgct gatctcgaaa agagcatgga gtggtttggc
     4021 ggaaggaaca ccgagcgcgg gatcctgctg ttcatcgtgc gtcactccgc cgagcatctg
     4081 ggccagtcga tcgcctacgc tcgctcgatc ggcgttgtcc cgccctggac cgaagacttg
     4141 aagaagcagg cggacaagcc cgctccagcc aaaaaatagc ggcaaggagc cccggacgcc
     4201 ccgtcgaggg acatctagcc acgggacgtt tcttgctgcc cggcggacga ggcgtccgcc
     4261 gctccacttt ttgaccacgc cctcaatgcc gccgggtgtg ctagattcgg agacttggta
     4321 gaagtcttcg aatggacccg gcacacacca tctctaccca gggcctgacc cgccgcttcg
     4381 gcgatctcgt tgccgttgag ggcgtcaacc tgcgcgtggc gccggggcag ttcttcggtt
     4441 tcctcggccc caacggcgcc gggaaatcga ccaccatcaa gatgctgacc gggctgctgg
     4501 cgcccagctc cggccgcatc gaaattcttg gcctcgacct cacgactcat ccggtcgagg
     4561 tgaaacgcca gattggtgtc gtgccggaag gcatggctct gtttggacgc ctcaccggcg
     4621 ccgagttcct gagcttcgcc ggacgcatgt atggcctcga tcgccagacc gcggcccagc
     4681 gcgcgaccga actgctcgaa ttcatgcaac tggccgatca gcccaagaaa cttgtcaccg
     4741 attattccca cggcatgcag aaaaagctcg ccatggcggc cgcagtgatt catggcccca
     4801 aggtgct
//
LOCUS       DASPUL010000525         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_777644, whole genome shotgun sequence.
ACCESSION   DASPUL010000525 DASPUL010000000
VERSION     DASPUL010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777644"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VEJ00_12345"
     CDS             complement(<1..875)
                     /locus_tag="VEJ00_12345"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HXX01997.1"
                     /translation="MLRAFFVHLSENRSLRNFAERSKIGQRVSGRFVAGTEIADAVRV
                     TQAVNGAGMSVTIDNLGENVTNPDEARESARLYHQILEAIAANHLNANISLKLTHMGL
                     DVDEAMARDLVSGLVSKSASMNPPGFVRVDMEGSPYTQRTLDFVHELHRMPGNGNSVG
                     AVIQAYLKRSESDIEKLLAERIRIRLCKGAYKEPASIAYESKADVDANYIKLMKILMK
                     SGIYHGLATHDESIIREAQAFATRENLARDSFEFQMLYGIRRDLQRRLVRDGWRMRVY
                     IPFGTEWYPYFMRRLG"
     gene            complement(1044..1976)
                     /gene="trxB"
                     /locus_tag="VEJ00_12350"
     CDS             complement(1044..1976)
                     /gene="trxB"
                     /locus_tag="VEJ00_12350"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HXX01998.1"
                     /translation="MSETRNVVIIGSGCSGHTAALYTARANLKPLVIEGHEPGGQLSL
                     TTLVENFPGFPEGILGPQLVENMRKQAERFGAEYRMAHLSSADLSKRPFLLHLGHQQV
                     HTQTLIIASGASARWLGLPHEQQLIGHGVSSCATCDGFFFSGREIVVIGGGDSAMEEA
                     IFLTRFATKVTVIHRRDAFRASKIMLDRAQHNPKIQFLLDTVVEDVYDVGKQEVTGLK
                     LRNVKTGKVWDFPTSALFLGIGHEPNARMFAGQLETDADGYLKTHNYVLTRVPGVYAC
                     GDVQDRRYRQAITAAGTGCMAAIEAERFLEEHVK"
     gene            complement(2057..>2272)
                     /locus_tag="VEJ00_12355"
     CDS             complement(2057..>2272)
                     /locus_tag="VEJ00_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX01999.1"
                     /translation="LARCSAAEGGQPVDQSAYEPLFRGASDVLANYRGLLSVRSVVLE
                     ELAKLGGESKPKAAPKAKKAGPAADKK"
BASE COUNT          437 a          673 c          693 g          469 t
ORIGIN      
        1 cccagccgcc gcatgaaata cggataccac tcggtcccaa acgggatgta gacccgcatg
       61 cgccagccgt cgcgtaccag tctcctctgc aaatcacgac gaatgccgta gagcatctgg
      121 aattcgaacg aatcgcgtgc caggttttcc cgggtcgcaa aggcctgagc ttcccggatg
      181 atgctctcgt cgtgcgtggc caggccgtgg tagatgccgc tcttcatcag gatcttcatc
      241 agcttgatgt agtttgcatc gacatcggct ttgctttcat aggcgatgct ggccggctcc
      301 ttgtaggcgc ccttgcacag acgaatacga atgcgttccg ccagcaattt ctcgatatcg
      361 ctctcggatc gcttcaggta ggcctggatc actgccccga cgctgttgcc gttccccggc
      421 atgcgatgca gttcgtggac aaaatcgagc gtgcgctgcg tatacggcga accttccatg
      481 tcgacgcgca cgaagcccgg cgggttcatg gatgctgatt tggacacgag gccgctgacc
      541 aggtcgcgag ccatggcctc atctacatcc aggcccatgt gggtcagttt caggctgatg
      601 ttggcgttga ggtggttggc agcgatggcc tcgagtatct gatggtagag gcgcgcactc
      661 tcgcgggcct cgtcaggatt ggtgacgttc tcgccgaggt tgtcgatggt gacgctcatc
      721 cccgcgccat tcaccgcctg ggtaacgcgt acggcgtcgg cgatctccgt tccggcgacg
      781 aagcggccgg agactcgctg cccgatcttc gaacgctcag cgaaattgcg cagggaacgg
      841 ttttccgaga ggtgaacgaa aaatgccctt agcatgaaac acctgccggg tccggaagag
      901 acgagtccgg aaagaatgga agaaaaatgc agggatcctg catgaagatc acagaaagtt
      961 gtatcacaaa gcgcccgaat caaacgtgac tcagcgcact acaccgccgc tttaagcggg
     1021 ttgccctgaa ccggggcctg gtatcacttc acgtgttctt ccaggaacct ttccgcttcg
     1081 atcgcggcca tgcatcccgt gccggcggca gtgatggcct ggcggtagcg gcggtcctgc
     1141 acatcgccgc aggcgtagac gccgggaacg cgcgtcagaa cgtagttgtg tgtcttgagg
     1201 tagccgtcgg cgtcggtctc gagctggccg gcgaacatcc tggcgttggg ttcgtggccg
     1261 atcccgagaa aaagcgcgct ggtagggaag tcccacactt tgcccgtctt tacgttccgc
     1321 agcttgagtc cggtgacttc ctgcttaccc acgtcgtaga cgtcttccac gacggtatct
     1381 agcaggaatt ggatcttggg attgtgctgc gcgcggtcaa gcatgatctt cgaggcgcgg
     1441 aatgcgtcgc ggcgatgaat gaccgtcacc ttggtcgcga agcgggtgag gaagatcgct
     1501 tcctccatcg ctgagtcgcc gccgccgatc actacgattt ctcttccgga gaaaaagaag
     1561 ccgtcgcagg tggcgcacga ggagacaccg tgcccgatga gctgctgctc atgcggcagc
     1621 cccagccacc gcgccgaggc gccgctggcg atgatcagtg tctgggtatg aacctgctga
     1681 tggccgagat ggagtaagaa agggcgctta ctcaggtcgg cgctgctgag atgagccatc
     1741 cgatactcgg ctccgaagcg ctccgcctgc ttgcgcatgt tctcgaccag ctgtgggccc
     1801 aggatgcctt ccgggaatcc agggaagttc tccaccagcg tggtgagaga aagctgcccg
     1861 cccggctcgt ggccttcgat gacgagaggc ttcaggttgg cacgagcggt gtagagggca
     1921 gcggtgtggc cagaacatcc cgacccgata atcacaacgt ttcgagtttc gctcatggaa
     1981 tttgctgcag attagatggc tcagttcaga tttggatgca ctgcgccttt gatcgcagcc
     2041 gacagggaat gtccagtcac ttcttatccg ccgcgggccc ggctttcttc gccttcggag
     2101 cagcttttgg tttcgactcg ccgccgagct tcgccaattc ctcgaggacg accgaacgca
     2161 ccgacaacag gccgcgataa ttcgccagca catccgaggc gccgcggaac agcggttcgt
     2221 aggcactctg atccacgggt tgaccccctt ccgccgccga acagcgagcc ag
//
LOCUS       DASPUL010000526         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1744284, whole genome shotgun sequence.
ACCESSION   DASPUL010000526 DASPUL010000000
VERSION     DASPUL010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744284"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VEJ00_12360"
     CDS             complement(<1..530)
                     /locus_tag="VEJ00_12360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXX02000.1"
                     /translation="MALEVHIEKKLAEFTLDVSFAADNEPLGILGPSGAGKTMLLRCI
                     AGLDDPNRGRIILGNHTLLDTDNGVRVPARKRRIGMLFQHYALFPHQSVRDNVGFSLG
                     RLPRAAREQRVRATLAQVHAADLEPRYPRHLSGGEQQRVALARALASEPEALLLDEPL
                     SALDTHLRSQMEAQLR"
     gene            complement(532..1224)
                     /gene="modB"
                     /locus_tag="VEJ00_12365"
     CDS             complement(532..1224)
                     /gene="modB"
                     /locus_tag="VEJ00_12365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HXX02001.1"
                     /translation="MAPLEATDWSPLWISLKTSVTATLITLVAGLAAAAWRERHKGPS
                     VALVDGICLLPMVLPPTVVGFFLLLLFGRSGPLGRLLLQMGTTVIFSWPATVIAAAVV
                     AFPLMYLSARAALEQVDSHFLEAARTLGASEWRVFGQIALPLAWPGVLAGTILSFARA
                     LGEFGATLMLAGNIPGRTATIPIAIYFAVEADDLRSAFAWCGADVVISLALLSGLYYW
                     THARGPARWARR"
     gene            complement(1315..1998)
                     /locus_tag="VEJ00_12370"
     CDS             complement(1315..1998)
                     /locus_tag="VEJ00_12370"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HXX02002.1"
                     /translation="MRLFFVTLQALCLAGPVLAQAPTTGAPQKAPQHQPGVAEPSHTP
                     NPDAKSAPSHSADKIDPEKEAAVRHLMDITETSKLGDNIATYLKGQVHDGVGRAIGPD
                     KVDAFMGTFSRKFSATSPATAVTDAMVPIYAKAFSMEDIQGLIQFYESPLGQRVVKAL
                     PDVVQQSQMAGVQIEQASALKVLREMTDEYPELKQVLPPENEQPAPPAAPAPAPGQAP
                     KPPPSAPPK"
BASE COUNT          435 a          678 c          740 g          426 t
ORIGIN      
        1 tcccggagct gcgcttccat ttgactgcgc aggtgcgtgt cgagagcgga gagaggctcg
       61 tccagcaaaa gagcctcggg ttcgctggca agcgcgcggg ccagtgcgac gcgctgctgc
      121 tcgccgccgg aaaggtggcg cgggtagcgc ggttcgaggt cggccgcatg gacctgcgca
      181 agcgtggccc gcacgcgctg ctcgcgtgcg gcgcggggca gtcgccctag gctgaacccc
      241 acgttgtctc gcaccgactg gtgagggaaa agagcgtagt gctgaaagag cattccaatg
      301 cggcgcttgc gtgcaggaac acgcacacca ttgtcggtgt caagcaaggt gtggttgcca
      361 aggataatgc ggccgcgatt gggatcatcc aacccggcaa tgcaccgcag cagcatcgtc
      421 tttccggcgc cagacggacc gagaattccg agcggttcgt tatcggccgc aaaggaaaca
      481 tccagcgtaa actcggctag ctttttctcg atgtgaactt ccagtgccat gtcaacgccg
      541 ggcccagcgc gcaggccccc gcgcgtgggt ccaatagtag aggccactca gcagggcgag
      601 cgaaatcacc acatctgccc cgcaccaggc aaacgcactg cgcaggtcgt cagcttccac
      661 cgcaaaataa atggcgatcg gaatcgtagc cgtccttccc ggaatgtttc cggcgagcat
      721 cagtgtggct ccaaattcgc cgagggcgcg tgcaaaggaa agaatcgtcc cagccagcac
      781 gccgggccat gccagcggca aggcgatttg tccgaagacg cgccattcgg aggcacccag
      841 cgtccgtgca gcttccaaaa agtgcgaatc aacctgctcg agggccgcgc gagcgctgag
      901 atacatcagc ggaaatgcaa cgaccgccgc cgcgattacg gtagcaggcc aggagaagat
      961 caccgtggtg cccatctgga gaagcagccg tccgagcggg ccggaccgcc cgaacagtag
     1021 cagcaggaaa aaacccacga ccgtgggtgg cagcaccatg ggaagaaggc agatgccgtc
     1081 gaccagggcc accgatgggc ccttgtgtcg ctcgcgccaa gccgccgcag caagccccgc
     1141 aaccagagtg atcagggtcg ccgtgacgct ggttttgaga gatatccaca gcggagacca
     1201 atcggtcgct tccagcggcg ccatgggcaa aggattatag cgagctcatg acggtttcgt
     1261 tcgctttttc cagcattgca agcgaggtcg ggggtgctcc cgcccgggtg ttgcttactt
     1321 gggaggggcg ctgggaggcg gtttgggggc ctggccgggc gctggcgccg gagcggctgg
     1381 cggcgcgggt tgctcgtttt ccggtgggag gacctgtttc aattccggat attcatcggt
     1441 catttcccgc agaaccttca gtgccgacgc ttgttcgatc tgtacgcccg ccatctgcga
     1501 ctgctgtacg acgtccggaa gagccttgac cacgcgctgg ccgagggggg attcgtagaa
     1561 ctgaatcagg ccttgaatat cttccatcga gaaagccttc gcatagatcg gaaccatggc
     1621 atcggtgacg gccgtggcgg gagaggttgc ggaaaatttc cgactgaagg ttcccataaa
     1681 cgcatcgacc ttgtcggggc cgatggcgcg tccgacacca tcgtgaacct gtcccttcag
     1741 ataggtcgcg atattgtcac cgagtttcga cgtttccgtg atgtccatca ggtgacggac
     1801 ggcggcctcc ttttccggat cgatcttgtc ggccgaatgc gagggggctg attttgcatc
     1861 cgggttgggg gtgtgcgatg gctccgccac accgggctgg tgctgcgggg ctttctgcgg
     1921 tgccccggtc gttggcgctt gcgccagcac gggccctgcc aagcaaagcg cttgcaatgt
     1981 gacgaaaaat aatctcatgt gcctctccta atggctggcg ttgcaaggtg acactcatca
     2041 tatcgaaggc caagcccagc gtcacggctg tttgcggagt cgtgccggac agagatcttg
     2101 ccgaggcctc ccgtggtgcc ttcagacggg gctgtgatac cttcagtgat tatatgctcg
     2161 gttcccaggc ccgtatcacg cacggccaag cctcgcctcg ggtaatcgca gctgtgctct
     2221 gtcgacctgc ggcgactatg aggcggcctg ccgccgcatg gttgcttgcc ggtctcgcg
//
LOCUS       DASPUL010000527         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1324374, whole genome shotgun sequence.
ACCESSION   DASPUL010000527 DASPUL010000000
VERSION     DASPUL010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324374"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VEJ00_12375"
     CDS             complement(<1..533)
                     /locus_tag="VEJ00_12375"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXX02003.1"
                     /translation="MSDDILTLPPPPADARIAYGSDANQFIDLRLPAGREKTAPPLPL
                     VINIHGGFWRAKYNLDHAGHLCAALAAKGVATANLEYRRVGNEGGAWPGTFSDIRTAY
                     AFLVQNARTHNFDPLRILVMGHSAGGQLALCLAAHEPGVKRVISLAGVVDLQRAYQLH
                     LSNDAVVEFLRGTPSEVP"
     gene            737..1066
                     /locus_tag="VEJ00_12380"
     CDS             737..1066
                     /locus_tag="VEJ00_12380"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HXX02004.1"
                     /translation="MFARILEVTPRMEKKDEFIKTVRQQIHPILKKQPGFLEMVPLIP
                     EAADAKWIALTLWTEKHHAETYLREVYPKVEQIMKPFLVGEALFRTFNVETSVCEHLV
                     EALTTHA"
     gene            complement(1156..2505)
                     /locus_tag="VEJ00_12385"
     CDS             complement(1156..2505)
                     /locus_tag="VEJ00_12385"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX02005.1"
                     /translation="MTQFPNSALQLEPPVAEAAADQTRQRTRNPLSPIVASQRDLLRQ
                     AVSAMRHDMRRTLLTMAGMAWGIATVVLLLAYGNGFARAITNIFESFGATAVGVFPGR
                     TSQQAGGNKAGVQIRFTNDDIELLRNNLPLARHVSRMAEKQSTVQNNNRSFTFPALGL
                     DPSIEDIWNLDVAEGRFLNDADNQQHGPYAVLGSEARDRLFSGMPAVGETIRVAGVTF
                     QVVGVLKPRMQESDDDDNRVVYIPYSSMDAVKDTHYLDGMWLDSAGLDHEKMAKTIRE
                     TLATAHGFKPDDQRALFVFDAQKQLSQFGIISAMLKILLGVIGTVTLSIGGVGLMNIM
                     LVSVTQRTREIGVEKALGARKRDVGFQFLAEAMVITALGGLLGILLSYAVSLSVGRLT
                     LYSAMAKHAEAGDIRLVVSPVIVLVATSILAVVGVISGMIPALRAANLDPIEALRYE"
     gene            complement(2530..>2746)
                     /locus_tag="VEJ00_12390"
     CDS             complement(2530..>2746)
                     /locus_tag="VEJ00_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02006.1"
                     /translation="TLLSGGIGIGVAAAIMHALAGVDMGTFDPPKLVASTATLALISL
                     TVAGVAAGLYPARRAALLEPVEALRKE"
BASE COUNT          552 a          845 c          833 g          516 t
ORIGIN      
        1 ggaacctcgc tcggcgtacc gcgcaagaac tccacgacgg cgtcattgct gaggtgaagc
       61 tggtaagcac gctgcagatc gacgactccc gcgagcgaga tgacgcgctt gacgccgggc
      121 tcgtgcgcag ccaggcacag cgcaagctgt cctcccgccg aatggcccat gaccaggatt
      181 cttagcgggt caaaattgtg tgtgcgagca ttctgcacga gaaaggcgta agcggtgcga
      241 atatcggaaa aagttcccgg ccaggcgccg ccttcgttgc ccacgcgccg gtattccaga
      301 ttggcagtcg cgactccctt ggcggccaat gctgcgcaca gatgtccggc gtgatccaga
      361 ttgtacttcg cccgccagaa cccgccgtgg atattgatca cgagcggaag cgggggcgcg
      421 gtcttttccc ttcccgcagg caggcgcaga tcgatgaact ggtttgcgtc cgagccatag
      481 gcgatgcggg catcggcggg cggcggaggc aaggtgagga tgtcgtcgga cataactcac
      541 cctcaattct aacgggccgg gtgctcctgg tcacgcggag gtgtggctgg aatcactgca
      601 cagaatcaat ctggcgagta tattcggccc cgattccaat ccaattcaac gacctgtttc
      661 gggattgcgc tcgcagggag ccgtctcgaa cggaggcttc ctccgaacac cttcccccta
      721 ggaaggagag tctcctatgt ttgcacgtat tctcgaagtt actcccagaa tggaaaagaa
      781 ggacgagttc atcaaaaccg ttcgtcaaca gattcaccca attctcaaga aacagcccgg
      841 attcctggag atggtacccc tgatcccgga ggcggccgac gcaaagtgga tcgcgctgac
      901 cctctggacc gaaaagcatc atgccgagac atacctgaga gaggtttacc cgaaggtgga
      961 gcagatcatg aagcccttcc tggtcggcga ggccctcttc cggaccttca acgtggagac
     1021 cagtgtgtgt gagcatctgg tcgaggcact gaccacccac gcctagacgg cgcccccgac
     1081 ttaagccgga gcacaactcg ctccggcttt ttctttgccg tttgattgct tgagcgacgg
     1141 gaagagcgca gtttcctatt cgtagcgcaa ggcttcgatg gggtcgagat tcgcggcccg
     1201 cagggcaggg atcatgccgc tgatgacgcc caccaccgcc agaatcgacg ttgcgaccag
     1261 cacaatgaca ggggaaacca ccaggcggat atctccggcc tcggcgtgct tggccatggc
     1321 actatatagc gtcagccgcc ccacagacag agaaacggca taagacagca ggatgcccag
     1381 cagcccgccc agggcagtga tgaccatggc ctcggcgagg aattgaaagc cgacgtcccg
     1441 cttgcgggcg cccagtgcct tttccacgcc aatttcgcgc gtccgctgcg tcaccgagac
     1501 cagcatgatg ttcatcagcc cgacgccgcc aatactcagt gtcaccgtgc cgatgacacc
     1561 aagaagaatc ttcagcatgg cgctgatgat gccgaactgc gagagctgtt tctgcgcgtc
     1621 gaaaacgaag agggcacgct gatcgtcggg cttgaagcca tgggcggtgg ccagagtctc
     1681 gcggatggtt ttcgccatct tctcgtgatc caggcccgcc gaatccagcc acatgccatc
     1741 gaggtagtgc gtgtccttga ccgcgtccat cgagctgtag gggatgtaga ccacgcggtt
     1801 gtcgtcatcg tcgctctcct gcatgcgagg cttgagcacg ccgactacct ggaacgtcac
     1861 gccggcgacg cggatggtct ctcccaccgc aggcatgcct gaaaacagac gatcgcgcgc
     1921 ctccgaaccg agcacggcat atgggccatg ctgctggttg tcggcgtcat tcagaaagcg
     1981 gccctcggcg acgtcgagat tccagatgtc ctcgattgat gggtccaggc caagcgccgg
     2041 aaacgtaaat gagcggttgt tgttttgcac cgtcgattgt ttttccgcca tgcgcgacac
     2101 gtgccgggcc agcggcagat tgttgcgcag caactcgatg tcgtcgttcg tgaagcggat
     2161 ctgcactccg gccttgttgc ctccggcttg ctgcgaggtg cgtccgggaa aaacgccgac
     2221 ggcggtggcc ccgaagctct cgaaaatgtt ggtgatggcg cgggcgaatc cgttgccata
     2281 ggcaagcagc agcaccaccg tcgcgatgcc ccacgccatg cccgccatgg tcagcagggt
     2341 acggcgcatg tcgtgccgca tggccgaaac cgcctgccgt aacagatcgc gctgggaagc
     2401 gacgatcggc gacaaaggat ttcgggtgcg ctgccgagtc tggtcggcgg cggcctccgc
     2461 cactggcggc tcaagctgca acgcgctgtt gggaaattgt gtcatttcca ttttcgttcg
     2521 gctattgccc tattccttgc gcagggcctc gaccggctcc agcagcgccg cgcgccgcgc
     2581 tggataaaga ccggccgcta ctccggccac cgtcagtgaa atcagcgcca gcgttgccgt
     2641 cgaggccacc agcttgggcg gatcgaaagt gcccatgtca actcctgcga gagcatgcat
     2701 gatcgcggcc gcgaccccaa tcccgatgcc tccgctgagc aaggtc
//
LOCUS       DASPUL010000528         5298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_508455, whole genome shotgun sequence.
ACCESSION   DASPUL010000528 DASPUL010000000
VERSION     DASPUL010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508455"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VEJ00_12395"
     CDS             complement(<1..368)
                     /locus_tag="VEJ00_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02007.1"
                     /translation="MRFCAAVCSLFVLFIAGSGQDKSATPNARKSFTVPATIDHNRVI
                     IDVDVPLPDGSSQRVHAWVDNGNPELVLSRHLATRLGLNVSCDDKECSAPPPREISIG
                     GMTIPLADVKNAEIPLRPVSA"
     gene            498..1370
                     /gene="mtnP"
                     /locus_tag="VEJ00_12400"
     CDS             498..1370
                     /gene="mtnP"
                     /locus_tag="VEJ00_12400"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HXX02008.1"
                     /translation="MPQAEIGIIGGSGLYSMPGLTKVKEVRLETPFGTPSDAYVCGTL
                     EGRKVAFLARHGRGHRILPSELNFRANIHGLKQLGVERIISISAVGSLKEEHKPLDFV
                     IPDQFFDRTRHRVDTFFGGGVVAHIAFADPVCGQLAAVVEAACKKIGVVGKRGGTYLC
                     MEGPQFSTKAESNVYRSLGMDVIGMTNLQEAKLAREAELCYVTVAMVTDYDCWHPEHD
                     SVTVNQVVAVLMKNAENAAKVVRETVAAMPRERSCKCGSALAHAILSDPKKIPAAAKK
                     RLRLVLGGYLKKSG"
     gene            1462..2385
                     /locus_tag="VEJ00_12405"
     CDS             1462..2385
                     /locus_tag="VEJ00_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXX02009.1"
                     /translation="MSILVVGSVAYDDVSSPSGSVKGILAGAATYFSLAASYFTQVRV
                     VAVVGEDFGAEQEKVFLKRGIDIRGIEHAKGRTFRWGGTYGENLNEAKTNFTELNVFE
                     GFQPRIPEEYQDTEFLFLANIQPVLQSDVRRKMNGVRLTGCDTMNYWIQRTPRELAET
                     LKLVDVLLINDAETKMLAKETNLPRAANKVLAMGPQALVIKHGEYGATIFFREGAFGI
                     GRHPFRAPTLPIEEVTDPTGAGDSFAGGFMGYLASQGEVNREVLKRALFYGGVMGSFA
                     VERFGTERLQSLTREEIDARFQIFRELTHLE"
     gene            2456..4150
                     /locus_tag="VEJ00_12410"
     CDS             2456..4150
                     /locus_tag="VEJ00_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02010.1"
                     /translation="MRKPAKNTPTPTPWDSEVQYLLWLATTVSVFSFLFYFQRGEVLL
                     YGDAVAHINIARRVFDSKTPGLLQLGTVWLPLPHLLMIPFLVSDDLWRRGAGGSMPSM
                     AAYVFAVVGIFRLVRGALFRGREPDAAARGAAWTAALIFGSNPNLIYMQTTAMGEVLY
                     LAFFLWTIVFFSEWVRGDAKALTKCGLCQMAACLTRYDGWLLAAVLVGTALARKMFAA
                     RATDLEHSRIANPQLARFVLLAAAAPALWLAYNGIIYRNPLEFENGPYSAKAIERKTQ
                     ITGNPGHPGTGNPPLAGLYFVKSGEVNVAENEGLQRSWVVLAVAGLVGIIVSLRRPNP
                     SDDSPSEDTRHPSAWTMLFLLVPLPFYALSVAYGGVPIFVPPFWPHTHYNVRYGLQLL
                     PAFAAAMAAVVYLAVKSRRLPSKARIVVVLAPIAFVTVSYASIWRGGPVCLREAQINM
                     HIRNQFEAQVAGWLEKLPPDSTLLMYLGDHVGAVQQAGIPLKHVINEGNHRVWKRPVD
                     PDGLWERALSDPARYADYVVAFEGDPVWQAVQGRHLKSLVEIHATGQSPAVLYRAR"
     gene            4266..5066
                     /gene="dapF"
                     /locus_tag="VEJ00_12415"
     CDS             4266..5066
                     /gene="dapF"
                     /locus_tag="VEJ00_12415"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXX02011.1"
                     /translation="MNPGSRTIPFVKATACGNDFLLIEGALAPPDVADFTRCICDRHE
                     GVGADGVEWMFPHFSVDVEVRLINGDGSAAEISGNGTRCVAAYLCSTQGKERISILTG
                     AGTKVCALTARQDSEYEFEIEMGAATVDNELALQTRGAEVRGLPVSIGNPHFVVFVPQ
                     FPDDWQNRALAIQRCAEFNQGVNVEWVVVNGKHDISARFFERGVGETRSSGTGSCAAA
                     VAAMASRQSDSPVKVHASGGTQTVRREGNNIFLRGPARIVCRGEFLLS"
BASE COUNT          996 a         1594 c         1633 g         1075 t
ORIGIN      
        1 gcgctgaccg gccgcaaggg aatctctgcg ttctttacat ccgcaagggg gatcgtcatc
       61 ccgccgatgc tgatctcgcg cggcggcggt gccgagcatt ccttgtcatc gcaggagaca
      121 ttgagtccca gccgcgtggc caaatggcgc gagaggacaa gctcgggatt gccgttatcg
      181 acccaggcat gcactctctg gcttgagccg tcaggcagag gcacatccac gtcgatgatc
      241 acgcggttat ggtcgatcgt ggcgggaaca gtgaacgact tccgggcgtt cggtgtcgcg
      301 gatttgtcct gccctgaacc cgcaataaac agtacgaaca gcgagcaaac cgcagcgcag
      361 aacctcatag atgaattgta gcgccgctcg tccggctgat tccggccgtg aagagtcctg
      421 cgcgccggca gacccgtgac tgccgtcttg tataatcacc ggtttggctg cgcgcgcagt
      481 gccgcgtcgg aggggctttg ccacaagcgg agatcggtat tatcggcggg agcgggctgt
      541 actccatgcc cgggttgacc aaagtcaaag aagtgcggct agagacgccc ttcggtactc
      601 cgtccgatgc ttatgtatgc ggcacgctcg agggccgcaa ggtcgccttt ctggcgcgtc
      661 acggacgagg ccatcgtatc ctgcccagcg aactgaattt ccgcgccaat atccacggcc
      721 tcaagcagct cggcgtcgag cgcatcatct cgatttccgc cgtcggttcc ctgaaagagg
      781 agcacaagcc gctcgacttc gtaatccccg accagttttt cgatcgcacc cgccatcgtg
      841 tggatacctt ctttggcggc ggtgtagtgg cacacatcgc cttcgccgac ccggtgtgcg
      901 gccagctggc ggcagtggtc gaagccgcat gcaagaaaat cggcgtcgtc ggcaagcgcg
      961 gcggaaccta cttgtgcatg gagggtccgc agttctcgac gaaagccgaa tccaatgtct
     1021 atcgcagcct gggcatggac gtgatcggca tgaccaatct gcaggaagcc aagctggcgc
     1081 gcgaggccga actttgttat gtcaccgtcg ctatggtgac cgattacgac tgctggcatc
     1141 cggagcacga ctcggtgaca gtaaaccagg tcgtggccgt gctcatgaag aacgccgaga
     1201 atgcggcgaa ggtcgttcgt gaaaccgtcg ccgccatgcc ccgcgagcgc tcctgcaagt
     1261 gcgggtcagc cctggcccat gccattctgt ccgatcccaa gaagatcccg gcagcagcaa
     1321 agaagcgatt gagattagtt ctgggaggat atctgaagaa aagtggctag tggtcagtga
     1381 tcagtggtca gagagttgcc gcgtacacaa actcagggct ggaaattgac tactgatgtc
     1441 tgaccactga ccacagattc tatgagcatt ctcgtggtgg gttcggttgc gtacgacgac
     1501 gtctcttcgc cttcggggag cgtgaaagga atcctggccg gggcggcaac atacttctct
     1561 ttggccgcaa gttatttcac gcaggtgcgc gtcgtggctg tggtgggcga agattttggc
     1621 gctgagcagg agaaggtatt tctcaagcgc ggcatcgaca ttcgggggat cgaacacgcg
     1681 aagggtcgca cgtttcgctg gggcggtacc tatggcgaaa acctcaacga agcgaaaacg
     1741 aactttacgg aactgaacgt cttcgaaggt tttcagccgc gcatccctga agaatatcag
     1801 gacaccgagt tcctttttct ggccaacatc cagccagtcc tgcagtccga cgtgcggcgc
     1861 aagatgaacg gcgtgcgtct gaccggctgt gacaccatga attactggat tcaacgaact
     1921 cccagggagc tcgccgagac gctgaagttg gtcgatgtgc tgctgattaa cgacgcggaa
     1981 accaagatgc tcgccaagga gaccaacctg ccgcgcgcgg caaacaaggt cctggccatg
     2041 ggcccgcagg cgctggtcat caagcacggc gaatatggag cgacgatttt ctttcgtgaa
     2101 ggagccttcg gaattggccg tcaccccttt cgcgcgccga cgctgcctat tgaggaagtg
     2161 accgatccca caggcgcggg cgattccttc gccggcgggt tcatgggtta cctggcgtcg
     2221 cagggagaag tgaaccgcga ggtgttgaag cgggcgctgt tctacggcgg cgtcatggga
     2281 tcgttcgccg tcgagcgctt cggcaccgag cgcctgcagt cgctgacgcg cgaagaaatc
     2341 gacgcccgct tccagatctt tcgcgaactg acgcacctgg aatagacatg tagggacagc
     2401 tgcccccggc tgtccagtcg agcggaagct cgacggttcg cctaagcgag cacccgtgcg
     2461 caaaccagcg aagaacacgc ccactccgac tccctgggac tccgaagttc aatacctgct
     2521 gtggctcgcg acgaccgtct ccgtcttctc cttcctcttc tacttccagc gcggtgaggt
     2581 gctgctctac ggggacgccg tagcccatat caacatcgcc cgccgcgttt tcgattcgaa
     2641 aacccccggc ctgctgcagt tgggaaccgt ttggctcccg ctgccgcacc tgctcatgat
     2701 tccgtttctc gtctccgacg atctgtggcg gcgcggcgcc gggggttcca tgccgtcaat
     2761 ggccgcctat gtgttcgccg ttgtcgggat ttttcggctg gtacgtggag cgctcttccg
     2821 cggccgcgaa ccggacgccg ctgcgcgtgg cgcggcctgg actgctgcgt tgatcttcgg
     2881 atcgaatccc aacctgatct atatgcagac caccgccatg ggtgaagttc tgtatctcgc
     2941 gttcttcctg tggacgattg tcttcttcag cgaatgggtt cgcggcgatg ccaaggctct
     3001 caccaaatgc ggtctgtgcc aaatggcggc ttgcctgacg cgctacgatg gatggctcct
     3061 cgcggcagtt ctcgttggaa cggcgctggc tcgcaagatg ttcgctgccc gggcgaccga
     3121 tctcgagcat tccagaattg ccaacccgca acttgccaga ttcgtcttgc tcgcggccgc
     3181 ggcaccggcc ttgtggcttg cctacaacgg aatcatctat cgcaatccgc tcgaattcga
     3241 gaacggtccg tattccgcaa aggcgattga acgaaaaacc cagatcaccg gcaacccggg
     3301 acatcccgga accggcaatc cgccgctggc cggcttgtat tttgtgaagt ctggtgaagt
     3361 caacgtcgcc gagaacgaag ggctgcagcg ctcctgggtc gtcctggcgg tcgcgggttt
     3421 ggttggaatc atcgtgagtc ttcgccggcc caatccctcc gacgattcgc ctagcgaaga
     3481 cacgcgacat ccttcggcct ggaccatgct cttcctgctg gtcccccttc cgttctacgc
     3541 attgtcggta gcctacggtg gcgttcctat cttcgttccg cccttctggc cgcataccca
     3601 ctacaacgtt cgttacgggc tgcagttgct gcccgcgttc gcagcagcaa tggctgcagt
     3661 cgtatacctc gctgtgaaat cccgtcggct gccgagcaag gcgcgcatcg tagtcgtgct
     3721 ggctccgatt gcgttcgtca cagtaagtta cgcctcgatc tggcgcggcg gaccggtctg
     3781 cctgcgggaa gcacaaatca acatgcatat ccggaatcaa ttcgaggccc aggtggccgg
     3841 atggctcgag aagttgccgc ccgactcgac cctgctcatg tacctgggtg atcacgtcgg
     3901 agccgtgcag caggcgggca ttccgttgaa acatgtcatc aacgaaggta accatcgcgt
     3961 gtggaagcga ccggtcgatc ccgacggctt gtgggaacgt gctctctccg atcctgcccg
     4021 ctatgccgac tacgtcgtcg ccttcgaggg tgatcccgtc tggcaggccg tccaagggcg
     4081 gcacctcaag tccctggtcg aaatccacgc cacaggacag tcgccggccg tgctgtatcg
     4141 cgctcggtaa tcagccgggt aacgcaggcg attcttggcg gatgataaaa tccgcccatc
     4201 cagcgcatgt cgacagagac gatgagccga gggaccgcac gacgcctcga ccgggtggag
     4261 cgcgagtgaa cccggggagc cgcaccattc cttttgtcaa ggcgaccgcc tgcggcaacg
     4321 attttctgtt gatcgaggga gcgctggctc cgccagatgt ggccgacttt actcgctgca
     4381 tctgcgaccg gcatgaaggc gtgggcgccg acggcgtcga gtggatgttc cctcatttct
     4441 cggtggacgt cgaagtcagg ctgatcaacg gcgacggatc ggctgcagaa atttccggca
     4501 atggcacccg ctgcgttgcg gcctacctct gttcgacgca gggcaaggag cggatttcga
     4561 ttctcaccgg agcgggcacg aaagtttgcg ctctgaccgc gcgccaggac tccgaatacg
     4621 agtttgagat cgagatgggc gcggccacgg tggacaacga gcttgccctg cagacgcgcg
     4681 gcgccgaggt ccgcggcctg ccggtctcga tagggaatcc gcactttgtc gtttttgtgc
     4741 cgcagtttcc ggacgactgg cagaaccggg cactcgccat tcagcgctgt gcggagttca
     4801 accagggagt caatgtcgag tgggtagtcg tgaacgggaa gcacgacatt tcggcgcgtt
     4861 tcttcgaacg cggagttggt gaaacccgct cttccggcac gggatcgtgc gcggcggccg
     4921 tggccgcgat ggcaagccgg cagtccgact cgcccgtcaa agtgcacgcc tcgggcggta
     4981 cccaaaccgt gcgccgggag gggaacaata tttttcttcg cggcccggct cggatcgtat
     5041 gccgcgggga gttcttgctg agttgactgc tgactcatga atcggatttg aaagggcgcg
     5101 gcttccagcc gcgtcgtggt gccgacagcg taagtcagag ccttggtgaa ggcttcggat
     5161 ccatgccatc ttccaatcga caacgcgtca ggccgcccgc gcttcggccc ggggatagca
     5221 tcggtatcgt tgcaccggcc agcaatgtca agcaggccga tttcgaagcc ggctgcgaag
     5281 ccttgcgccg cgccggct
//
LOCUS       DASPUL010000529         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2021111, whole genome shotgun sequence.
ACCESSION   DASPUL010000529 DASPUL010000000
VERSION     DASPUL010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2021111"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VEJ00_12420"
     CDS             complement(<1..623)
                     /locus_tag="VEJ00_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXX02012.1"
                     /translation="MQRYRLYADPHNLFLNDLVLNSCRRNPQKTALIDASNGRRLTYA
                     EYGEIVESVARGMIAAGLKPGEVVAIFLANSWEFCAAFHGGALAGAIPTVLNPTYRER
                     EVRYQLENSGAAILITDGCNLEGINLSGLPALRRVYTTRTPAAGAEPFARLLERSTAA
                     FPRPEQSSEQTIAALPYSSGTTGLPKGVMLSHYNLVANVYQLIGPNAT"
     gene            complement(652..897)
                     /locus_tag="VEJ00_12425"
     CDS             complement(652..897)
                     /locus_tag="VEJ00_12425"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HXX02013.1"
                     /translation="MPELRNVVVYSRKGCHLCEVVKESLDKLSRRGGFTWHEIDVDAD
                     AELRREFTDQVPVVFIDGRKAFKYRMDEQEFLRKLSA"
     gene            1006..1776
                     /locus_tag="VEJ00_12430"
     CDS             1006..1776
                     /locus_tag="VEJ00_12430"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX02014.1"
                     /translation="MPDLEVKPLRGKTALVTGGARRLGRASALALAEAGADVAITYRH
                     SDREARETVIDLSAFRVRAFALRCDITDEASVRTMMKEAGRELGRIDILVNNAANYET
                     AEFEKLTVQQWDAIFASNTRGPFLVSREALKWMRRKRGKGQGRIEAKIINMGSLGGLR
                     PWATHAHYCSSKAALHMLTKVMAKALAPEIAVNAVAPGMIDLGEKSAAAFMRRMARQT
                     PMQRNGSGEEIARAVLFFATAPQFITGQILAVDGGLGL"
BASE COUNT          402 a          573 c          691 g          387 t
ORIGIN      
        1 gtggcgttgg gtccgatcag ctgataaacg ttggccacga ggttgtagtg cgagagcatg
       61 actcccttgg gcaacccagt agtgccgctg gagtagggaa gcgccgcgat cgtctgctcg
      121 gaagactgct cgggcctggg aaacgccgcc gtcgagcgct cgagcaagcg cgcaaacggc
      181 tccgcgcccg ccgcgggggt gcgcgtggtg tagacgcgac ggagcgccgg taatccggag
      241 aggttgatgc cttccagatt gcagccgtcc gtgatcagga tggcggcgcc ggagttctcg
      301 agctggtaac gcacctcgcg ttcccggtag gtcgggttga ggaccgtagg gatggctccg
      361 gccagggccc cgccatggaa tgcagcgcaa aactcccagg agtttgccag gaagatagcg
      421 accacctcac ccggcttgag tccggcggca atcatgccgc gggcgacgga ttccacaatt
      481 tccccgtatt cggcgtaggt caggcggcgg ccgttggaag cgtcgatcag cgcagttttt
      541 tggggattgc gacggcagga attgagaacc aggtcgttga ggaaaagatt gtgaggatcg
      601 gcgtaaagtc ggtagcgctg catcgccgga gattgtagcg aaaaaaccgg cctatgcgct
      661 cagtttgcgc aggaactcct gctcgtccat gcggtacttg aaggcctttc gtccgtcaat
      721 aaaaacgacc gggacctggt cggtgaattc gcggcggagt tcagcgtcgg cgtccacgtc
      781 gatctcgtgc caggtaaaac cgccgcgccg cgagagcttg tcgaggctct ctttcacgac
      841 ctcgcacagg tggcaccctt tgcgcgagta gaccacgacg ttgcgaagct ccggcatgtc
      901 gggagattct agcgcgggga attgcggcat ttccagcggg aaagtgaatt ttcccctatc
      961 ggtgttcgca gggtcgccga ttcgggcaag gggtagaatt cctgcatgcc agaccttgag
     1021 gtgaaacctc ttcgcggcaa gaccgcgctg gttacaggcg gagccaggcg cctggggcgg
     1081 gcgtcggcgc ttgccctggc ggaagctggt gcagacgtcg ccatcaccta ccggcattcc
     1141 gaccgtgagg cccgggagac cgtcatcgat ttgtccgcct tccgggtgcg cgcgtttgcg
     1201 ctacgctgcg acatcacgga cgaggccagc gtacggacga tgatgaagga ggctggcagg
     1261 gaattggggc gcatcgacat cctcgtaaac aatgccgcga actacgagac cgccgagttc
     1321 gagaagctga ccgtgcagca gtgggacgcg attttcgcct ccaacacgcg cgggccattt
     1381 ctggtttcgc gggaagccct gaagtggatg cggcggaaac ggggcaaagg ccaggggcgg
     1441 atcgaagcga agatcatcaa catgggttcg ctgggcggac tgcggccatg ggccacccat
     1501 gcacactatt gctcctcgaa ggcggcgctg cacatgttga ccaaggtcat ggccaaagcg
     1561 ctggcaccgg agatcgcagt gaatgccgta gcgccgggga tgatcgacct gggagagaag
     1621 tcggcggctg cgtttatgcg gcgcatggcg cggcaaaccc ccatgcagcg caacggcagc
     1681 ggcgaagaga ttgcccgtgc cgtactgttc ttcgcgaccg caccgcaatt catcaccggg
     1741 cagattctgg ccgtggatgg cgggctgggt ttgtagagct gttagtttcc gaggtcgatc
     1801 gcgttactta agggccaaaa aaactcgcct cgggtcgagt tcttgagagg gcgtggcttc
     1861 aagccgcgcc gtgaactaga accagtctca taaacatcct gagcgctgga aatcatgcat
     1921 cctgaaaatg gccggtggga ctccacatcc gatggggagc cttgggagtt gaccggctat
     1981 cctcgcccgt tgtccggaag cacgacgacg cctgtttcgc ttgtgagaat catgatgaag
     2041 cctttgcttc tcg
//
LOCUS       DASPUL010000530         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1874328, whole genome shotgun sequence.
ACCESSION   DASPUL010000530 DASPUL010000000
VERSION     DASPUL010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1874328"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..2097)
                     /locus_tag="VEJ00_12435"
     CDS             complement(130..2097)
                     /locus_tag="VEJ00_12435"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXX02015.1"
                     /translation="MQPRLLAISGPLKDSTIPLPEGEAALGRDATNAVPVVDPSVSRK
                     HCVLRREEDGRYQIHDLDSRNGTVVNGVPVKEHWLRHGDEIAVGDSVFLFLLEEAERP
                     APASRVEFDDTRPTAETKLIHPREVVYLQPDRLIRELPASSQVARNLGALLRISRVVH
                     AIRDLGELQTQLLDLIFEVVPASRGAILLTEASSQEFSSLYARTRKPGQPQTVRVSRT
                     IARQVMKENVAILGVDVPSTAPLRDVESLVASEVRSLLCVPLTVFQRVIGCIYLDSTS
                     PGERFEEDQLQLMVAVAGISAVALDNARRLQWLEQENQRLITEVGQDQSLVGESVTIK
                     EIYQFLAKVAPTDSTVLIEGESGTGKELAARALHRNSGRSTKPFIAINCAAIPESLLE
                     SDLFGHERGAFTGAATQKKGRLEIADGGVVFLDEIGELAPALQVKLLRVLQEREFERV
                     GGTHPIKVDIRLIAASNRDLSQAVSDGQFRRDLYYRLAVVKMRMPALRERREDIPLLA
                     RHFVQKYAKRCKVKAKPISREAMSALLNHDWPGNVRELENAIERALVLGSSDVLLVED
                     LPEALLEHAQPEDITEGKYHALVKEFKKELIVDAVVQTRGNYSEAAGILGVHPNYLHR
                     LIRNLGLKDALNEALRAGSRGLLRKTTGGAA"
     gene            complement(2498..>3295)
                     /locus_tag="VEJ00_12440"
     CDS             complement(2498..>3295)
                     /locus_tag="VEJ00_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02016.1"
                     /translation="PITFFAREEADWLGLGKNPHFSQNRGEVGHPGSETGSGLSHGAQ
                     LVHEFLRQRGASFFADIVRGTGKLKAEIETALWELVAAGVVTADGFDNLRSLIDPKRR
                     AGQGSGRTMRPRHSTGRWALLHADVAVERPRAVEAACWMLLRRYGIVIRDLLARESNL
                     PPWRELLMGFRRLEDRGEIRGGRFVDGFLGEQFALPVAVESVRAMRSLPLSGESMTLS
                     AADPLNLVGILVPGERVPAISGKTVSYRDGIAIQPAEQTPASEAVAG"
BASE COUNT          616 a         1119 c          918 g          642 t
ORIGIN      
        1 gaaccggccg cgccaccggc ccgcatcttc acgttgccgt ccgctggcag ggcacgtatc
       61 tcgatcctgc gcggctgatg cagctgtcgt tgccctgacg gcggccttaa gcaagatcca
      121 ccttctgacc tatgccgctc cccctgtcgt cttgcgcagc aggcctctcg accccgcgcg
      181 caacgcctcg ttcagagcgt ctttcagccc aagattccgg atcagccgat gcaggtagtt
      241 ggggtgcacg cccagaatgc cagccgcctc ggaatagttg cctctggttt gcaccacggc
      301 atcaacgatc agttccttct tgaattcctt cacgagagca tggtatttgc cctcggtgat
      361 gtcctcgggc tgagcatgtt ccagcaatgc ctccggcaaa tcttcgacca gtaatacgtc
      421 tgacgagccc agcaccagcg cccgctctat cgcgttttcc agttcgcgca cgttgccggg
      481 ccagtcgtga ttcaggagag ccgacatggc ttcgcgcgaa atcggcttcg ccttcacctt
      541 gcagcgcttg gcatatttct gcacaaaatg tcgcgccagc aagggaatgt cttccctgcg
      601 ttcgcgcagc gccggcatcc tcatcttgac taccgccagc cggtagtaca aatcccgccg
      661 gaactggcca tcgctcaccg cctggctcag atcgcggttg ctggcggcga tcaggcggat
      721 atccaccttg atgggatggg tcccgccgac gcgttcgaat tcgcgctcct gcaacacgcg
      781 taacagcttc acctgcagcg ccggagccag ctcgccaatc tcatccagaa aaaccacacc
      841 accgtctgcg atctccagcc gccctttctt ttgcgtcgct gccccggtga aggctccgcg
      901 ctcgtggccg aacaggtcgc tctccagcag agattcggga atggccgcgc agttgatggc
      961 tatgaaaggc ttggtggacc ggccgctgtt ccgatgcagc gcgcgggcag ctaactcctt
     1021 gcccgtgccg ctctcgcctt cgatcagaac ggtagaatcg gtcggcgcga ccttggccag
     1081 aaactggtaa atctccttga tcgtcacgct ttctcccacc aggctttgat cctgcccgac
     1141 ctcggtgatg agacgctgat tctcctgttc cagccactgc agccgccggg cattgtccag
     1201 cgcgaccgcg gagattccgg cgacggccac catcagctgc aactggtcct cctcaaatcg
     1261 ctcgcccggg ctggtgctgt ccaggtaaat gcatccgatc acgcgctgaa acacggtgag
     1321 cggtacacat agcaacgagc gtacctcgga agccaccagg ctctccacgt cgcgcagggg
     1381 agccgtggac ggcacgtcga cgcctagaat cgccacgttc tctttcatca cctgacgcgc
     1441 gatagttcgg ctgacccgca ccgtctgtgg ctgccccggt ttgcgcgtcc gggcatacag
     1501 agagctgaat tcctggctgc ttgcctccgt cagcaggatg gccccgcggc tggccggcac
     1561 gacttcaaaa atcagatcca ggagctgtgt ctgcagttcc cccagatcgc gaatggcatg
     1621 gacaacgcgg ctgatccgga gcagcgcccc caggttgcgc gccacctgcg aactggctgg
     1681 cagctcccgg atcaggcggt ccggctgcag gtaaaccacc tcgcgaggat gaatcagctt
     1741 ggtctccgca gtcggacggg tgtcgtcgaa ctccacgcgg ctggccgggg ccggccgctc
     1801 tgcctcctcg agcaaaaaga gaaagaccga atctcccacc gcgatctcgt cgccatggcg
     1861 cagccagtgt tccttcactg gcacgccatt gaccacggtg ccattgcggc tgtccaggtc
     1921 gtggatctgg tatcgcccgt cttcttcgcg ccgcaacacg cagtgcttgc gcgacaccga
     1981 gggatcgact accggcactg cgttggtcgc atcccggccc agggcggctt caccctcagg
     2041 caagggaatc gtcgagtcct ttagcggtcc cgagatggcc agtagtcgcg gttgcaaatc
     2101 gcggtcctcc tgaagtaagg tggctttctt accacggaat cgccacaaac gttctcccga
     2161 tcccggcgaa tttccaatat ccgaacagtt agtgagacta tccgattgga tagttaatag
     2221 ggcacaatac cggcccgcac acttgcccgt tgaaacccac ctgacagtgc taatcgattg
     2281 ttggagaatg gtttacatcg catcgggaat tgacttcacc ctgcccagaa gttggaaccc
     2341 ggcgtgcaca ggtcggaaag tacggatgct cggcaaagcg acgggggaag cgctttcgat
     2401 cagtacctgg ccgagatcgc caagggtcag aggctcgggg gccccctcat cgagcttctg
     2461 acttttttgt gctctccttc tcgccaagag atcagtccta cccggccact gcctcgcttg
     2521 ccggagtttg ctcggccggc tggatcgcaa tcccgtcccg atagctgact gtcttacccg
     2581 aaatcgccgg cactcgttcg cccggaacga gaattcccac gaggttcaaa ggatcggcag
     2641 cggatagagt catgctctcg ccgctcaagg gcaagctgcg catcgcacgc acggactcga
     2701 ccgccaccgg cagagcgaac tgctcgccca gaaatccatc cacgaatcgc ccgccgcgaa
     2761 tctcgccgcg gtcctccagc cggcggaatc ccatcagcag ctcgcgccag ggaggcaagt
     2821 tggattcacg cgctagtaga tcgcggatca caattccgta acgccgcagc agcatccagc
     2881 acgcagcttc gactgctctt ggccgctcca cggcgacatc ggcgtgcagc aatgcccaac
     2941 gtccggtgct gtgccggggc cgcattgtgc gaccgcttcc ctgccccgcc cgccgcttcg
     3001 gatcaatcaa agaccgcaga ttatcgaagc cgtcggcagt cacaacccca gccgcgacca
     3061 actcccagag cgcagtctcg atctccgcct tcaacttgcc cgtgccgcgc acgatgtcag
     3121 caaaaaacga tgctccccgc tgccgcagga actcatgtac cagctgcgcc ccatggctga
     3181 gaccactgcc cgtctccgag cccgggtgcc ccacttctcc ccggttttgg gagaagtggg
     3241 gatttttgcc gagccccagc cagtccgctt cctcgcgagc gaagaaagtg atcgg
//
LOCUS       DASPUL010000531         4035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1467420, whole genome shotgun sequence.
ACCESSION   DASPUL010000531 DASPUL010000000
VERSION     DASPUL010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467420"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1338)
                     /locus_tag="VEJ00_12445"
     CDS             complement(85..1338)
                     /locus_tag="VEJ00_12445"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXX02017.1"
                     /translation="MLTLALSELLGATVYDPSGAASGRVREVTLAPQEDRSRVSSLIV
                     KTKSGNRVLPFSSVSAINGGVKAVTPSGEWPPANGTEGLFLLERDLLDQQVIDINGRK
                     VVRVNDVDLQMDIPRDLTHPRPVLRVHSVDVGARGAVRRLLRGVAPRTALHALLGRIP
                     PRSIPWNFVDLIETDPARRVKLKISHDGLAKLHPADIADIVEELAPDERQAVFQTLDE
                     EVAAEALEEVEPRVQKSIVESLDSERAAEIVEEMNPDAAADLLGELPEERTEQILVQM
                     EPEAQQEVVELLEHKEQTAAGRMTTEFLALPVAATVQNAIESMREFEGGVEAVSTIYL
                     VDSHGTLVGAVPLARLVLAQPGTAMLSLTQEPLILSHEGVEENEVAELFDKYNLLTLP
                     VVDERNKLTGVITSDDVISMLRAKL"
     gene            1615..2862
                     /locus_tag="VEJ00_12450"
     CDS             1615..2862
                     /locus_tag="VEJ00_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX02018.1"
                     /translation="MRLIIDIFGQTLRTLWAHKLRSVLTMFGIAWGVGSLLLLVGVGE
                     GFRSGNRKQFDQIGQNILFIWSGRAPVMQGSFTALRQYYLTYDDYLDVLRDCPSVAQA
                     AAVIRRSDIRAVSDFYQASGQIMGVPAMFNDIRYLPMNEGRWLNTMDDAQKRRVIVLG
                     DEARRTLFLGRPSVGSTILLNGIRFEVIGTLKRIGHGDNTNQNLQSFVPLSTMRENFP
                     PTNVGEASGVISFINYQPRSRALHETAMQEVHKVIARNHGFDPNNPDSFDEWDTVQTS
                     DEVGKIFDAMNTFLGSVGLVTLALGAIGIINIMLVAVADRTREIGLRKALGATRASIM
                     FQFFVEGAFLTLLSGGIGIGVAAAIMHALAGVDMGNFDPPKLVASTATLALISLTVAG
                     VAAGLYPARRAALLEPVEALRKE"
     gene            2886..>4035
                     /locus_tag="VEJ00_12455"
     CDS             2886..>4035
                     /locus_tag="VEJ00_12455"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX02019.1"
                     /translation="MRQFPNSALPLEPPAAEAAADHTRQRTRNPLSPIVASQRDLLRQ
                     AVSAMRHDMRRTLLTMAGMAWGIATVVLLLAYGNGFARAITNIFESFGATAVGVFPGR
                     TSQQAGGNKAGVQIRFTNDDIELLRNNLPLARHVSRMAEKQSTVQNNNRSFTFPAIGL
                     DPSIEDIWNLNVAEGRFLNDADNLQHGLNAVLGSEARDRLFSGMPAVGETVRVDGVTL
                     QVVGVLKPRMQEGDDDDNRVVYIPYSSMDVIKDTHYLDGMWLDSAGLDHEKMAKTIRE
                     TLATAHTFKPDDQRALFVFDAQKQLSQFGIISAMLKILLGVIGTVTLSIGGVGLMNIM
                     LVSVTQRTREIGVEKALGARKRDVGFQFLAEAMVITALGGLLGILLSYA"
BASE COUNT          769 a         1259 c         1260 g          747 t
ORIGIN      
        1 aaactgccgc tacaaacgac cgaacaggat aaacgcagcc cgcgaatctg gccaaagaaa
       61 aaatccggat gagggtgaac ttacctacag tttcgcccgc agcatgctga tcacgtcgtc
      121 cgaggtgatc acgcctgtga gcttgttgcg ctcgtcgacc acggggaggg tgagcaggtt
      181 gtacttgtcg aagagttcgg ccacttcgtt ttcctcgaca ccctcgtggc tgaggatcag
      241 gggctcctgg gtgagggaca acatcgccgt gccgggctgg gccagaacga gccgtgccag
      301 cggcacggcg cccaccagcg ttccatggga atcgacgagg tagatggtgc tgaccgcctc
      361 cacgccacct tcgaactcgc gcatggactc gatggcattc tgcaccgtcg ccgccaccgg
      421 caaagcgagg aactccgtgg tcatgcggcc ggcggcggtt tgttccttgt gttcgagcag
      481 ttcgacaact tcctgctgcg cttccggctc catctggaca agaatctgtt cggtgcgctc
      541 ttccggcagc tcgccgagca ggtcggcggc ggcgtccggg ttcatctcct cgacaatctc
      601 tgccgcgcgc tcggaatcga gcgattcgac aatggatttc tgcacccttg gttccacttc
      661 ctccagggcc tcggcggcca cctcctcgtc gagggtctgg aagacggcct ggcgctcgtc
      721 aggcgccagc tcttcaacga tgtcggcgat gtcggcgggg tgcagcttgg ccaggccgtc
      781 atgcgagatt ttcagcttca cacgccgggc gggatcagtt tcgatcaggt cgacaaagtt
      841 ccacggaatg ctgcgtggcg ggattctgcc cagcagcgcg tgcaaggccg tccgcggcgc
      901 taccccgcgc agcaggcggc ggaccgcgcc ccgggccccg acgtcgacgg aatgcacgcg
      961 caacaccggc cgggggtggg tcagatcccg tggaatatcc atctgcaggt cgacatcatt
     1021 gacccgcacc accttgcgcc cgttgatgtc gatcacctgt tgatcgagga gatcgcgctc
     1081 gagcaggaag agtccctcgg tgccattggc ggggggccat tcgccggacg gcgtcacggc
     1141 cttgacaccc ccgttgatag ccgacaccga cgaaaatgga agcacgcggt tgcccgactt
     1201 ggtcttcaca atgagcgaag agacgcggga gcgatcctcc tgcggcgcga gggtcacttc
     1261 gcgcacccgg ccgctggccg caccggacgg gtcatagacc gtggcgccca gcaattcgct
     1321 aagggcaagc gtcaacatcc cgctagagca taaccaactc ctgtggaaat cggaacgggt
     1381 ttttgcacca aattgtcctc ggggagaccg taggaggagc cagctgcgta gactttcgac
     1441 cctacgcgtc cgaggttacg agttcccaat cttgcaccgc cggggcgtgc caggcccgaa
     1501 gccccggcag ccaagtctcc acaaataaca accgtctagc cttggcctcg gaggaagaca
     1561 attgggcacc gggagccgga actgagagcg aacgacaccc gtataattga gtgagtgcgg
     1621 ctcatcatcg atattttcgg ccagacgctg cgcacgctct gggcgcacaa gctgcgctcg
     1681 gttctgacca tgttcgggat cgcctggggc gtaggctcgc ttctactgct ggtgggagtg
     1741 ggcgagggct tccgctccgg caaccggaag caattcgacc agatcgggca gaacatcctg
     1801 ttcatctggt cgggacgagc cccggtcatg cagggcagct tcaccgcgct gcgccagtac
     1861 tacctgacct atgacgacta cctggacgtc ctgcgcgact gccccagcgt agcccaggcc
     1921 gcagccgtaa tccggcggag cgacattcgg gcggtcagtg acttctacca ggccagcggc
     1981 cagatcatgg gcgtgccggc gatgtttaac gacatccgct atctaccgat gaacgaaggc
     2041 cgctggctca acaccatgga tgacgcgcag aagcgccggg tcatcgtgct gggcgatgag
     2101 gcgcggcgca ccctgttttt gggacggcct tcggtcggca gcacgattct gctgaacggc
     2161 atccgctttg aagtcattgg cacgctcaag cgcattggcc acggggacaa taccaatcag
     2221 aatctgcaga gcttcgtgcc cctgagcacc atgcgcgaga acttcccgcc gaccaacgtg
     2281 ggcgaggcca gcggcgtgat ctcgttcatc aactaccagc ccagatcgcg cgccctgcac
     2341 gagaccgcca tgcaggaagt acacaaggtc atcgcgcgca atcacggttt tgaccccaac
     2401 aacccggact cgttcgatga atgggacacg gtgcaaacgt cggatgaggt gggcaagatc
     2461 ttcgatgcca tgaacacctt cctcggcagc gtggggctgg tgacgctggc cctgggagcg
     2521 atcgggatca tcaatatcat gctggtggcg gtggccgacc ggacgcgcga aatcggcctg
     2581 cgcaaggccc tcggtgccac ccgggccagc atcatgttcc agttcttcgt ggaaggggcg
     2641 tttctcacgt tgctcagcgg gggcatcggg attggggtcg cggccgcgat catgcatgcg
     2701 ctcgcaggag ttgacatggg taatttcgat ccgcccaagc tggtggcctc gacggcaacg
     2761 ctggcgctga tttcactgac ggtggccggg gtagcggccg gtctttatcc agcgcggcgc
     2821 gcggcgctgc tggagccggt cgaggccctg cgcaaggaat agggcaatag ccgaaggaaa
     2881 tggaaatgag acaatttccc aacagcgcgt tgccgcttga gccgccagcg gcggaggccg
     2941 ccgccgacca cactcggcag cgcacccgaa atcctttgtc gccgatcgtc gcttcccagc
     3001 gtgatctgtt gcggcaggcg gtctcggcca tgcggcacga catgcgccgt accctgctga
     3061 ccatggcggg catggcgtgg ggcatcgcga cggtggtgct gctgcttgcc tacggcaacg
     3121 gattcgcccg cgccatcacc aatattttcg agagctttgg cgcgacggcc gtgggcgttt
     3181 ttcccggacg cacctcgcag caagccggag gcaataaagc aggagtgcag atccgcttca
     3241 ccaacgacga catcgagttg ttgcgcaaca atttaccgct ggcccggcac gtctcccgca
     3301 tggcagaaaa gcagtcgacc gttcagaaca acaaccgctc gtttaccttc ccggcgattg
     3361 gcctggaccc ttctatcgag gacatctgga atctcaacgt tgccgagggc cgctttctca
     3421 atgatgccga caaccttcag catggcctga atgccgtcct cggttcggag gcgcgcgacc
     3481 gcctgttttc gggcatgcct gcggtgggag agaccgtccg cgtggacggc gtgactttac
     3541 aggtcgtagg cgtgctcaaa ccgcgcatgc aggaaggaga cgatgacgac aaccgcgtcg
     3601 tctacatccc ttacagctcg atggatgtga tcaaagacac ccactacctc gacggcatgt
     3661 ggctggactc ggcgggcctg gatcacgaga agatggcgaa aaccatccgc gagaccctcg
     3721 ccaccgccca caccttcaag cccgacgatc agcgtgccct tttcgttttc gacgcgcaga
     3781 aacagctctc gcagttcggc atcatcagcg ccatgctgaa gattcttctc ggtgtcatcg
     3841 gcacggtgac tctgagtatt ggcggcgtcg gactgatgaa catcatgctg gtctcggtga
     3901 cgcagcggac gcgcgaaatt ggcgtggaaa aggctctggg cgcccgcaaa cgggacgtcg
     3961 gctttcagtt cctcgccgag gccatggtca tcactgccct gggcgggctg ctgggcatcc
     4021 tgctgtctta tgccg
//
LOCUS       DASPUL010000532         4663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1195722, whole genome shotgun sequence.
ACCESSION   DASPUL010000532 DASPUL010000000
VERSION     DASPUL010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195722"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..572
                     /locus_tag="VEJ00_12460"
     CDS             39..572
                     /locus_tag="VEJ00_12460"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HXX02020.1"
                     /translation="MTLNSDQLKELIREVPDFPKQGILFYDITTLLKDKVGFATLIDK
                     FSEYYISQNVDLVLGMEARGFIFGPALAYRLNAGFVPVRKPGKLPAATVKQEYALEYG
                     LASLEIHKDAIQKGQRVLIVDDLLATGGTAEATANLALSLGAEIAGLGFVVELDFLKG
                     REKLKNYDVMALLHYDK"
     gene            complement(671..937)
                     /locus_tag="VEJ00_12465"
     CDS             complement(671..937)
                     /locus_tag="VEJ00_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02021.1"
                     /translation="MSHKLQSLRVSTDKILAKIVAQDSGRAQLRGGSNLQILRSWYEY
                     DSEAELARPRRRLNWNMLLGAVIVVGISASFWTGVGILVARIWK"
     gene            complement(1207..1464)
                     /locus_tag="VEJ00_12470"
     CDS             complement(1207..1464)
                     /locus_tag="VEJ00_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02022.1"
                     /translation="MFARKIRVEYEDAGRRLPCPLKWLDSFAMRNFTNASVFDDTLPV
                     ADGQMEIGANVPLVELTAAMEDWFRRKGYLTKASKLLLAES"
     gene            1898..2146
                     /locus_tag="VEJ00_12475"
     CDS             1898..2146
                     /locus_tag="VEJ00_12475"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXX02023.1"
                     /translation="MISNLIWWAVVGLIAGWAAGRIMKGGGYGVVMDIVLGIIGAVIG
                     GWLLGVLGIHAGGFIGTIIVAIFGAVILIWITRLLKKA"
     gene            2565..>4663
                     /locus_tag="VEJ00_12480"
     CDS             2565..>4663
                     /locus_tag="VEJ00_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02024.1"
                     /translation="MRAKTAVLKTAVLWLALGLVLAPTFSGRALLAQSGGPSPSNPAA
                     GASPEGAARLPVTRVILYKNGVGYFEHAGHVRGSQDVNVDFTTAQLNDVLKSLTVLDL
                     GKGRITGVSYNSTAPLERRLGMLRLPVGENPTTAQFLDALRGARVEVRSGGASATGRL
                     LSIEEREEAGKSEAAKNNGNDDDNSSHKVAQTQVSIVTDNGEVRVFNLTPATSVRIAE
                     KDVNEEIGKYMGVVASTRDQDVRRMTISTAGNGERDLLISYISEVPVWKSTYRIVIPK
                     EGKPLLQGWAIVDNTVGEDWKNVELSLVAGAPQSFVQELSQPYYTRRPVVPLPENAML
                     TPQTHEATMEEQAPPPVMGTGGVIGGVPGGVAGGAMAAPMVSRMARIASGSGAGIGSG
                     SGGGTGGGVFHVGALQPPSPPRPDATVVAETLEEGTSVGQSQELGDLFEYNLKDRVTI
                     RKNQSALVPILQSRIDAEKVSVWNPSESSVLRALWVDNSSSLTLDGGSFNVLEGDAFA
                     GEGLMDAIKPGEKRLLSYAADLGVLVDAKEKGEKQRVTKIAIAHGAMTQTTQERQENI
                     YTIRNRDTAARTVVIEHPARAGWKLTDDDDKPAESSASFHRFRLTIDPKTTKTLVVKE
                     YRPIDNRYQLTNVTDSDIKYFLDQKMINPDVEQALRRVVKQKNEVFLMEGIIKDRNAQ
                     VNAISNDQQRVRENMKAL"
BASE COUNT          946 a         1390 c         1448 g          879 t
ORIGIN      
        1 acggtctcag ttcatttcgc attgaaggag cgtggtaagt gacgcttaat tccgatcagc
       61 tgaaagagct tatccgcgaa gtccccgatt ttcccaagca ggggattctc ttttacgaca
      121 tcacgacatt gcttaaggac aaagtcggat tcgcgacgct catcgacaag ttctccgagt
      181 actacatctc gcaaaacgtc gacctggtgc tgggcatgga ggcccgcgga ttcattttcg
      241 gccccgcgct cgcctatcgt ctgaatgccg ggttcgttcc cgtgcgcaag cccggcaagc
      301 tgccggccgc cacggtgaag caggagtatg cgctcgaata cggcctggct tcgctcgaga
      361 ttcacaaaga cgcgatccag aaaggccagc gcgtgctgat cgtcgacgac ctgctcgcca
      421 ccggaggcac cgccgaagcc acggccaatc tcgcgctctc gctgggagcc gaaatcgcgg
      481 gcctgggctt tgtggtggag ctcgatttcc tcaaagggcg ggagaagctg aagaactacg
      541 acgtgatggc tttgctgcac tacgacaagt gaaagaaagt ttttgattgt tgaattttga
      601 ttgtggaatg aaaccaccaa ggctggagcc atggcatttc cattcaacag ccaaaatcaa
      661 tagtcgattc ttacttccag atccgcgcga ccagtatccc caccccggtc cagaagctgg
      721 cgctgattcc cacgacgatc accgcaccca ggagcatgtt ccagttgagg cgtcgtctgg
      781 ggcgggcgag ttcggcttcg ctgtcgtact cataccagga gcgcaggatc tgcaggttgc
      841 tgccgccgcg aagttgggcg cggccgctat cctgagcgac gatcttggcg agaatcttgt
      901 cggtgctcac tcggagggat tgtagcttgt gactcatgta gtcctctgcc ccgtcgctgc
      961 ttttttagag ccccattccc attttgggag tgtcagcccg aggtcaagcc cttgtaaatt
     1021 gtgctggagc caccactgta tgacgacccg gcggcagaat caagcgtcct gttacgacag
     1081 cggcaacatc tttgaggaaa gcaacatgac gcctggcggg aggttgccat ccgcaagcgg
     1141 cgtttcaggg tgcaaccacg gtttgctgga cgatccggga gtgggcagcg gttctgtagt
     1201 gcttggtcag ctctccgcca gcagcagctt cgacgcttta gtcagatacc ccttgcgccg
     1261 gaaccagtcc tccatggcgg cggtgagttc cacaagtggg acgttcgcgc cgatttccat
     1321 ctggccatcc gcgaccggca gcgtgtcgtc gaagacgctg gcgttggtga agttgcgcat
     1381 ggcgaagctg tcgagccact tgagcgggca gggaagccga cgtccggcgt cttcatactc
     1441 cacgcgaatc ttgcgcgcga acatagagtg atttaaacac aggggctcgg gaccaggggc
     1501 agtggacagc ggcggggtat gttgctcatg tggaagagcg gcccttcagg gcctgccctg
     1561 agcaagtgca acgccccgaa ggggccgcgt caggctctat gaagtgggcg gttttaggac
     1621 ctgaggtcca ccggggctaa agccccaaat gcttcttgcc cgcaacgcgg ggctaaagcg
     1681 ccgctcttcc acggcagcac ccgtcgctga ttttagctgg ccgatctcaa gtccccctga
     1741 cccctgaccc tagtcggtac cgtttgacac caccacatcc ttcccgtact ctcctcgcac
     1801 gtattcggac ttctagcgct gtgcccagtg cagtcgcgca accgcgccgg cttcatttgc
     1861 accgaaacga cgaaccaaga cgacacagga ggatcttatg atcagcaacc tgatttggtg
     1921 ggcagtcgtt ggtctgatcg caggctgggc ggcaggcaga atcatgaaag gcggtggtta
     1981 cggcgtggtc atggatatcg ttctcggcat catcggcgcc gtcatcggcg gatggctgct
     2041 gggagtgctg ggcatccatg ccggcggatt catcggcacg atcatcgtgg ccatctttgg
     2101 ggcggtcatt ctgatctgga tcacgcggct gctcaagaaa gcgtgaagtc aaggggtcag
     2161 gggccaggga tcagaggtca ccagcggcgt cagatgctcc cgtggaagag cggcccttca
     2221 gggccgcgtc acggtcctcc aagatctgag ggcctttagg ccctgcggtc caccggggct
     2281 aaagccccca aatgcttctt gcccgcaacg cggcgctaaa gcgccgctct tccacggcgg
     2341 tatcagtcgc cgattttagc tggccaacct caagcccttc tccctgaccc ctgcactcca
     2401 gccatctcaa accccagccg ttgcatccac cgactccaga tacctccgct caggtgtcta
     2461 aaccctgaga gcctgccttt ccgctgccgg cttggtcaat gcaagagcag cggcgtcact
     2521 cgttcccctc gctaggccgg ctctcttgaa cggaggattc attcatgcgc gcgaaaactg
     2581 ctgttctgaa gactgccgta ctttggttgg ctcttggctt ggttctggct cccactttct
     2641 ctggccgcgc cttgttggcc caatcgggtg gcccgtctcc ttcgaatccg gctgccggag
     2701 cgtcgcctga gggggctgcc cgcttgccgg tgacgcgggt gattctctac aaaaacggcg
     2761 tcggctactt cgagcacgcc gggcacgtgc gcggcagtca ggatgtcaac gtggacttca
     2821 cgacggcgca actgaacgac gtgctgaaat cgctgaccgt gctcgatctc ggcaaagggc
     2881 gcatcaccgg cgtcagctac aactcgaccg cgcccttgga acgtcgcctc ggcatgttgc
     2941 gcctgccggt aggagagaat cccaccacgg cgcagtttct cgatgcgctg cgcggcgcgc
     3001 gggtggaagt ccgcagcgga ggggcttcgg cgacgggcag gctgctgagc attgaagagc
     3061 gggaggaggc agggaagagc gaagctgcca agaacaacgg taacgatgac gacaattcat
     3121 cccataaagt cgcgcagaca caagtttcaa tcgtcaccga caacggcgaa gtgcgcgtgt
     3181 tcaacctgac gcccgcgacc agcgtgcgca tcgcggaaaa agacgtgaac gaagaaatcg
     3241 gcaagtacat gggcgtggtc gcgtccacgc gcgatcagga cgtgcgccgc atgaccatct
     3301 cgaccgcggg caatggcgag cgcgacctgc tgatcagcta catcagcgaa gtgccggtgt
     3361 ggaagagcac gtaccgcatc gtgatcccga aagagggcaa gccgctgctg cagggatggg
     3421 ccatcgtcga caacacggtg ggcgaagact ggaagaacgt ggagctttcg ctggtggcgg
     3481 gggcgccgca gtcgttcgtg caggaattgt cgcagccgta ttacacgcgg cgtccggtcg
     3541 tgccgttgcc ggagaatgcc atgctcacgc cgcagacgca tgaggcgacg atggaggagc
     3601 aagctccgcc gccagtcatg gggacaggcg gcgtgatcgg cggcgttccc ggcggagttg
     3661 caggcggggc gatggcagca cctatggtca gccgcatggc cagaatcgcc agcggctcag
     3721 gcgccgggat cggctctggc agcggcggtg ggacaggcgg aggtgtcttt catgtcggcg
     3781 ctcttcagcc gccgtcccct cctcgccccg atgcgaccgt agtcgccgag acgctcgaag
     3841 aaggaacctc ggtcggccaa tcgcaagaac tcggcgacct gttcgaatac aacctcaaag
     3901 accgggtgac catccgcaag aaccagtcgg cgctggtgcc gattctgcaa tcgcggatcg
     3961 acgccgaaaa ggtttctgtg tggaatccgt cggagagttc ggtgctgcgc gcgctgtggg
     4021 tcgacaactc gtcgagcctc acgctcgacg gcggcagctt caacgtgctc gagggcgacg
     4081 ccttcgcggg cgaagggctc atggacgcga tcaagcccgg cgaaaaacgc ctgctctcct
     4141 acgccgccga tctcggcgtg ctggtcgacg ccaaagagaa aggcgagaag cagcgcgtca
     4201 cgaaaatcgc tattgcgcac ggcgccatga cgcagaccac gcaggagcgc caggagaaca
     4261 tctacactat ccgcaaccgc gataccgcgg cgcgcacggt ggtcatcgag catccggcgc
     4321 gagcgggatg gaagctcacc gacgatgacg acaaacccgc cgagagttcg gcgtcattcc
     4381 accggttccg cctgaccatc gatccgaaga cgaccaagac gctggtggtc aaggaatatc
     4441 ggccgatcga caaccgctat cagcttacga atgtgaccga cagcgatatc aagtattttc
     4501 tcgaccagaa gatgatcaat cctgacgtgg agcaggctct gcggcgggtg gtgaagcaga
     4561 agaacgaggt cttcctgatg gagggcatca tcaaggaccg gaacgcgcag gtgaacgcca
     4621 tcagcaatga tcagcagcgc gtgcgcgaga acatgaaagc gct
//
LOCUS       DASPUL010000533         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_650366, whole genome shotgun sequence.
ACCESSION   DASPUL010000533 DASPUL010000000
VERSION     DASPUL010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650366"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VEJ00_12485"
     CDS             complement(<1..424)
                     /locus_tag="VEJ00_12485"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXX02025.1"
                     /translation="MKAVTWLLLLGSAAYAAECPSGQAKDEATLLQIENTWAQAAEKH
                     DLAALDCILASDFEEVGAEGQLIDRSHMLQSAADPQAVHYELLEMHAHVYGDAAYVRG
                     IGAAVRDGKMTPKTRFTDVFVYREGRWQCVAGQEARFLA"
     gene            692..>2066
                     /locus_tag="VEJ00_12490"
     CDS             692..>2066
                     /locus_tag="VEJ00_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02026.1"
                     /translation="MTAQNDKPLVIFPASPRIAALLLALLAFAVLTLTGCGSKNSPTT
                     TTTTNNPPPPPNTASSITKDGITWTFSQPVTVGQFVNGDYYVVGPVTVTAIYPAPTTS
                     SPYENGSVLNLPTTNSKSGFDSRLNDGTDESWWFDASLRSYPPIDLKPGDALVSTVSL
                     PQIHTDPEVMRASDMSASPVRSASVLTVLSAAPSADAFRPSYCDRTQTIYHANDLQRN
                     LLPSLAPPNPSQTPALSVFENYYRRPWIDTNPFLFDAPADYMPSYGQHIAFADSYASL
                     LLMLNFPASQKVNLTNYFVQYGIDLYGCVQRGYGWPAFGGHRSGRKLPIILAGILLNN
                     DGMKNVSTTYPNQFGEDMQTVYVNAIPGGYTQAWQGATVIYGGHYGVNADGTPVSAGL
                     YGPYEQLQPINWPVINGNEQLGEAYRRCCTSVSWIGEALAIHILKAESVWNHPAFFDY
                     TDRWMTED"
BASE COUNT          409 a          757 c          521 g          379 t
ORIGIN      
        1 cggccagaaa ccgagcttcc tgccccgcca cgcactgcca tcttccttcg cgatacacaa
       61 aaacatcggt aaatcgagtc ttcggtgtca ttttgccgtc gcgcacggct gcgccgattc
      121 cgcggacgta tgcggcgtcg ccgtagacgt gcgcgtgcat ctccaacagc tcgtagtgca
      181 cggcttgcgg gtcggcggcg ctctgcagca tatgcgagcg gtcaatgagc tggccttcgg
      241 ccccgacttc ttcgaagtcg ctcgccagga tgcagtcgag cgctgccagg tcgtgctttt
      301 cggccgcctg cgcccacgtg ttctcgattt gcagcagcgt ggcctcatcc ttggcctgtc
      361 ccgatggaca ctccgccgca tacgccgcag aacctaacag caacagccac gtgacagctt
      421 tcaacgcaat gctccttccg tcgcgaatca caaatgcctc atcataaacg gttgctcggc
      481 actgcggctc tgcgagagtg gcgcggtctc gtcagcaccc gtgcaccccg actacgtccc
      541 ggcccgtaat ccggacatcg gccgcctgac ctgccgaccc gcgattgcat cgtcccattg
      601 aacctcaccc cgcttgtccc tccgatactt gggcccacac ccgaaacggc gcatcctgaa
      661 agggctccaa gccaaacgag gggggcgcaa aatgaccgca caaaacgaca agccgttggt
      721 gattttcccc gcgtcccctc gcatcgcagc cctcctcctt gctctgctcg ctttcgctgt
      781 gctcaccctc accggttgtg gaagcaagaa cagccccaca acaacgacga ccactaacaa
      841 tccgcctcca cctccgaaca ccgcctcctc gatcacgaaa gacggcatca cctggacgtt
      901 cagccaaccg gttacagttg gccaattcgt gaacggagac tattacgtcg tcggcccggt
      961 caccgtcacc gccatctacc ctgcgccgac aacttcctct ccgtacgaaa acggctcagt
     1021 gttgaacctg cccaccacca acagcaaaag cggattcgac tcccgcctca acgacggcac
     1081 cgacgaatcc tggtggtttg acgcctccct gcgctcctac cctccgatcg acttgaagcc
     1141 cggcgacgcg ctggtttcga ccgtcagcct gccgcagatt cataccgatc ccgaagtcat
     1201 gcgggccagc gacatgagcg ccagcccggt gcgcagcgct tcggtgctga cggtgctatc
     1261 ggccgcgccg tccgccgacg ccttccgccc ttcgtactgc gaccgcaccc agaccatcta
     1321 ccacgccaac gacctgcaga gaaatctcct gccctcgctc gcgccgccga atccgtcgca
     1381 gactccagcg ctttcggtat tcgagaacta ctaccgccgc ccttggatcg acaccaatcc
     1441 cttcctgttc gacgcacccg ccgactacat gcccagctac ggccagcaca tcgccttcgc
     1501 cgactcctac gcttcactgc tgctgatgct gaatttcccc gccagccaga aggtcaacct
     1561 gaccaactac ttcgtgcagt acggcattga tctctacggc tgcgtgcagc gcggctacgg
     1621 atggcccgcc ttcggtggcc atcgcagcgg ccgaaaactg ccgattattc ttgccggaat
     1681 cctgctgaac aacgacggca tgaagaacgt ctcgaccacg tatcccaacc agttcggtga
     1741 agacatgcag accgtctatg tcaacgccat ccccggcggc tacacccagg cctggcaggg
     1801 cgcgaccgtc atctacggag gccactacgg cgtcaacgcc gacggcacgc cggtcagcgc
     1861 cggactctac ggtccctacg agcaactgca gcccatcaac tggccggtga tcaacggcaa
     1921 cgagcaactg ggagaagctt atcgccgctg ctgcacctca gtctcatgga ttggcgaggc
     1981 gctcgctatc cacatcctga aagctgagag cgtctggaac catcccgcgt tcttcgacta
     2041 caccgaccgc tggatgaccg aagacg
//
LOCUS       DASPUL010000534         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1195896, whole genome shotgun sequence.
ACCESSION   DASPUL010000534 DASPUL010000000
VERSION     DASPUL010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195896"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..417)
                     /locus_tag="VEJ00_12495"
     CDS             complement(157..417)
                     /locus_tag="VEJ00_12495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HXX02027.1"
                     /translation="MCLAVPGRVVEIIDGEDIAFRRGKVDFGGIRKDVNLAYTPEAEV
                     GKYVLVHVGFAISVIDEVEAQRVFEYLKEIGGLKEELGEIPQ"
     gene            complement(560..2806)
                     /gene="hypF"
                     /locus_tag="VEJ00_12500"
     CDS             complement(560..2806)
                     /gene="hypF"
                     /locus_tag="VEJ00_12500"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HXX02028.1"
                     /translation="MPIAAKPAVERLRITLCGAVQGVGFRPFVYRLASDMALSGWVLN
                     SSAGLVIEVEGSPEQLSRFEQRLERERPKASVVTLRESARIAPQGSTGFEIRASDSDT
                     GKTVNVLPDLATCTECREELFDPGNRRFEYPFTNCTNCGPRYTIVADIPYDRPNTTMR
                     GFVLCPKCREEYENPANRRFHAQPNACPVCGPKLDGTIEDAAEALLNGEIVGLKGIGG
                     FQLLVDARQPVAVARLRDRKHREEKPFALMMPSLEMARQYCEISPAEVELLESQAAPI
                     VLVQPKNATDLAWNVAHCSPYLGVMLPYSPLHHLLMQECRFPVIATSGNRSDEPIAIS
                     NEEAKERLGGIADRFLMHNRPIVRACDDSVVRLTRGRAGILRRARGYAPLGIRVRQNL
                     PPVLAVGGHLKNTVAIAVGQDVFLSQHIGDLDTLEARGAFEKAIADLCRLYSFKPELI
                     ACDLHPDYASTQWAERAGLPLVPVQHHQAHVAACAAENEVEGEYLGVSWDGTGYGLDG
                     AIWGGEFFHAKGNHFERIAHLRPFGLPGGDTAVREGWRSGASLLFETFGRFAEPRTPS
                     SGERSGRTRASAPPSDVATVQQMIERKINVIQTTSVGRLFDAVASITGIAQQNRFEGQ
                     AAMLLENEIGATRTEEAYALPDGNWGPLILALMADKRAGVATPLMAARFHNALVEWIL
                     EVAARAGLKQVVLSGGVFQNRYLTERAAAALESRGFTVYTHQRVAPNDGGIALGQAVM
                     AAAKAAVG"
BASE COUNT          524 a          920 c          920 g          550 t
ORIGIN      
        1 tgcaccagcg tgatctcgcg cggcagcagg tcttcgaggc cgctcttgac gatggtgtgc
       61 gtctgcccgc cgcagatttc catgatcgtc cacggacggg tcactttctt ccagatggcc
      121 tgcgcgagct tctcggccgc tctctggtcg cgatattcac tgaggaattt caccgagttc
      181 ctcttttagt ccgccgatct ccttcagata ctcgaagacg cgttgtgctt ccacttcgtc
      241 gatgacgctg atggcgaagc ccacgtggac gagaacatat tttccgactt ctgcttcggg
      301 cgtgtaggcg aggttgacgt ctttgcgaat gccgccgaag tcgaccttgc cgcggcgaaa
      361 ggcgatgtct tccccgtcaa tgatttcgac tacccggccg ggaacggcaa gacacatgga
      421 ctctatctcc gaaaaccgcc ggggctaaag cccctttttt ccgggctgct ctttacggcg
      481 cgcctcgaag gcgtgccctg acacgaagcg ggcaagcctt gtattcggca tggcttcagc
      541 cgtgccgtac tgcggggcct tatcccacgg cggctttagc cgccgccatc actgcctggc
      601 ccagcgcgat gccgccgtcg ttgggggcaa cccgctggtg cgtgtacacg gtgaacccgc
      661 gcgactccag cgccgccgcg gcccgctccg tgagataacg attctggaat accccgccgc
      721 tgagcacgac ctgcttcagc ccggcccgcg cagctacttc caggatccac tcgaccagcg
      781 cgttatgaaa ccgcgcggcc atgagtggcg ttgcaactcc cgcgcgtttg tccgccataa
      841 gtgccaaaat cagcggaccc caatttccat ccggtaaagc atacgcctct tcggtccgcg
      901 tggcgccaat ttcgttttcc agcagcatag ccgcctggcc ctcgaagcga ttctgctggg
      961 cgatgcccgt gatcgaggcg actgcgtcga agagacggcc aacactggtt gtctggatga
     1021 cgttgatctt gcgctcgatc atttgctgga ccgttgccac atccgaggga ggggcggacg
     1081 ccctcgttcg cccagaccgc tccccggacg agggcgtccg gggttccgcg aaacggccga
     1141 aggtctcaaa taggagactg gcccctgaac gccagccctc gcgcaccgcc gtatcaccac
     1201 cgggaagacc gaagggtcgc aagtgcgcga tgcgctcaaa gtgattccct ttcgcatgga
     1261 agaactcccc gccccagatg gcgccgtcga ggccgtatcc ggtgccgtcc caggagacgc
     1321 cgaggtattc gccttcaact tcgttctcgg cggcgcacgc ggccacgtgt gcttggtgat
     1381 gctgcacagg cacgagcggc agtccggcgc gctcggccca ctgcgtcgac gcatagtcgg
     1441 gatgcagatc gcaggcgatc agctcgggct tgaagctgta gaggcggcag aggtcagcaa
     1501 tggccttttc gaacgcgcct cgggcttcga gcgtatcgag atcgccgatg tgctggctga
     1561 ggaagacatc ctggccgacg gcgatagcga ccgtgttctt gaggtgtccg ccgacggcca
     1621 gcaccggcgg caggttctgg cgcacgcgaa tgccgagcgg agcgtagccg cgggcgcggc
     1681 gcaggatgcc ggcgcgcccg cgcgtgaggc gcaccacgga atcgtcgcag gcgcgcacga
     1741 tggggcgatt gtgcatcagg aaacggtcgg cgataccgcc caggcgctcc ttggcttctt
     1801 cgttggaaat cgcgataggc tcgtcgctgc gattgccgct ggtcgcgatt accggaaacc
     1861 ggcactcctg catcagcagg tgatgcagcg gcgaatacgg cagcatgacg ccgaggtaag
     1921 gagaacagtg agccacattc cacgctagat cggtagcgtt tttgggttga actaacacga
     1981 tcggtgccgc ctgcgattcc agcagctcga cctcggccgg cgaaatctcg cagtactggc
     2041 gggccatttc cagcgacggc atcatcaggg cgaatggctt ctcctcgcga tgcttgcgat
     2101 cgcgcagccg cgcgacggcg acaggctggc gcgcatcgac cagcagctgg aagccgccga
     2161 ttcccttcag cccgacgatt tcgccgttga gcagcgcctc ggctgcgtct tcgatggttc
     2221 catcaagttt cgggccgcac accgggcagg cgttgggctg cgcgtggaag cggcggttgg
     2281 cggggttctc gtattcctcc cggcatttgg ggcaaagcac gaagccgcgc atcgtggtat
     2341 tcggccggtc atagggaatg tcggcgacga tcgtgtaccg cgggccgcag ttggtgcagt
     2401 tcgtaaacgg atattcaaag cggcgattgc cagggtcgaa cagttcctcg cgacattcgg
     2461 tgcaggtcgc aagatcgggc aggacgttga cggttttccc ggtatcgctg tcgctggcgc
     2521 gaatttcaaa acccgtcgag ccctggggcg caattcgggc cgattcgcgc agggtcacca
     2581 ccgaagcctt gggccgctcg cgttccaggc gctgctcgaa gcggctcaac tgctcgggag
     2641 aaccctcgac ctctatgacc aggccggccg acgaattcag cacccagccg ctgagagcca
     2701 tgtccgatgc gagacgatag acgaaaggtc ggaacccgac accctgaaca gcgccgcaca
     2761 acgtaatgcg cagacgttcg accgcgggtt tggctgcgat tggcataggc gtcacgaatt
     2821 gcgcctgatc gccagattgt aagacctgga gcgcggaggc tgctatccgt cacgcgctgg
     2881 agattcaacc gcgccaaggt cgcaagcaaa tgac
//
LOCUS       DASPUL010000535         3624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_934275, whole genome shotgun sequence.
ACCESSION   DASPUL010000535 DASPUL010000000
VERSION     DASPUL010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934275"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VEJ00_12505"
     CDS             complement(<1..432)
                     /locus_tag="VEJ00_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02029.1"
                     /translation="MRKRLQIAWMLTVGTAIATSFSLNAAVVWAADGDGSHLVSTTDE
                     HGRTIYVNDDGPQKPRHAEAPAPRRSSLMYWSARENRWKPVPSAGTASMQAARSAAAE
                     VSQYFVRESAKSANARIIAANSHGHQASPEEVDASIVMAAAR"
     gene            complement(714..2978)
                     /locus_tag="VEJ00_12510"
     CDS             complement(714..2978)
                     /locus_tag="VEJ00_12510"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXX02030.1"
                     /translation="MRRLCLLFLLLLSTSPLSAQDTISVDAVSAMHVCSDKNPASAGP
                     CATAPRPLSRVNPSYPEKAREKRKEGIVTLGLTVNKDGSVSGVHVVKGVDKDIDQAAV
                     EAVSQWKFDPGTYQGNPVDVELAVEVNFRLGVNPRQAPPTGNLQEQKQAVDDFRNMYS
                     DATEAYSRGDYATAANLLRKITSLTPQSGSAWNELGRALMAMNELDAAAQAFQTSIKN
                     DPASRNAYNNLGLVYWRQRKYEEAAAQFNKQTLVNPDDHYAHRNLGMMLRDQRRCSEA
                     MPEIQKALSLTPNHAETLVAEGECDLDLGNRAKGLSELEQATSVSSAPNTFNSAAYVL
                     AKRNIEIGMAEKWSDTCLTIENTRLQNVSNAPLDHLTPDQLNYVFWMSAYWDTRGWIY
                     FLRGDNSSARSYVEAAWSLRTDPSVGDHLGHIYEKLDRSEDAAKLYAMAVASADQPTR
                     AKIDADDLADAKKQLEKLTEKNADSRIKQAGADLSAKNQVSVANEGGVSGSADFAVRI
                     SAGKPAEFHQLNGDKALAKFAESMQAVKLPVFVPESSGIEVPLRGTLTCHSEEAQCRF
                     AFVSPEEAVNLARNEMALASTTPAASGTHDPHVYDDPAMGIRIFLPDEWKLVRREPGS
                     FSVPRNAMFAKPGSAAMFMLTREHFEGSLELYQKMLDRFFATRTDFKRAGEETVKRDG
                     LTGTRWNVSWNEQGVVYSAVIEMFGEGDDYYRITTFAPKEVYDRYAETFENVLRSVQF
                     PMLRLNPHNLDPEK"
     gene            complement(3110..>3624)
                     /locus_tag="VEJ00_12515"
     CDS             complement(3110..>3624)
                     /locus_tag="VEJ00_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02031.1"
                     /translation="MVFTLEALTAEEIEQDLAEHRPEWKAAQRALVARLSQGAVGRAR
                     GFDLAGYIVARDHALTILNSALRGGDHSELFKATESYRPGAEGREKTEELIRTLYSLL
                     RDLMFIDSSAPNLVRNTDIASELKRLAESADFERITAASDRLAEVERGMRRNLLRSLS
                     LDAFAAGLEK"
BASE COUNT          698 a         1149 c          987 g          790 t
ORIGIN      
        1 ccgcgctgcc gccatcacga tagaagcgtc aacctcttcc ggcgaagcct gatggccatg
       61 agagttcgct gcaataatgc gcgcgttcgc cgatttcgct gactcgcgca caaagtactg
      121 gctcacttcc gccgccgccg accgtgccgc ctgcattgag gccgttcccg ccgagggtac
      181 cggcttccac cggttctccc gcgcgctcca gtacatcagc gacgagcgcc ggggcgctgg
      241 agcctcggca tgccgcggct tttgcggacc gtcatcgttc acatagatcg tgcggccatg
      301 ctcgtcggtc gtggaaacaa ggtgcgagcc atcgccgtcg gccgcccaca ccactgccgc
      361 attgagagag aaggaggtcg cgatggccgt gccaaccgtc agcatccacg caatttgcag
      421 gcgcttgcgc atatccgggc atcacctttc actgaacctt cgccgcaagg atgcgcgcca
      481 aacacacgct atcctcgctg ctatctaccc ggaacctgac ggagaatctt gaaaccagag
      541 taggtcgatt ataggcgggc cggaaaagcc gggcaatctc tccaaaggtc gctgaaccag
      601 cggagtaatc gcggtaacca gctgaaacgt aagtggatta gggaatttcg atatccggtg
      661 ctcaatcaac gggggtaacc gctcgatatg gttacctgcc atgccgactg gcgctatttc
      721 tcaggatcca gattgtgcgg attcagccgg agcatgggaa actgcaccga acgcaggacg
      781 ttctcgaatg tttccgcgta ccggtcgtag acctctttgg gcgcaaaggt tgtgattcgg
      841 tagtaatcat cgccctcgcc gaacatctcg atcacggccg agtacacgac tccttgttca
      901 ttccaactta cgttccagcg cgtacccgtt agcccatctc gtttcaccgt ctcctcaccg
      961 gcacgcttga agtccgtccg agtggcgaag aacctgtcga gcatcttctg atacagctcg
     1021 agcgaacctt cgaaatgttc ccgcgtcagc ataaacattg ccgccgagcc gggtttagca
     1081 aacatggcgt tccgcggcac gctgaatgat cccggctctc gcctcaccag tttccactca
     1141 tcgggcagaa agatgcggat acccatggcc gggtcgtcgt atacatgcgg gtcatgtgtc
     1201 ccgcttgcgg ctggggtcgt gcttgccagc gccatttcat tgcgcgccag attgacagct
     1261 tcctccggac tcacaaacgc gaagcggcac tgcgcctcct cggaatgaca ggtcaacgtt
     1321 cctcgcaggg gcacctcaat gccagaggat tccggaacga agacggggag cttgaccgct
     1381 tgcatcgatt cggcaaattt cgccagggcc ttgtccccat tcaattggtg aaactccgcc
     1441 ggctttccgg ctgagatccg gaccgcgaaa tctgcagagc cgcttacgcc gccttcgttg
     1501 gcaaccgaca cctggttttt tgcgctcaag tctgcgcccg cctgcttgat tctgctgtcg
     1561 gcgttcttct cggtcagctt ttccaactgt tttttcgcat cggccaggtc gtccgcatcg
     1621 atttttgccc tggtgggctg gtcggcggaa gccacggcca tcgcgtaaag cttcgccgca
     1681 tcctcggacc ggtccaactt ttcgtagata tggccgaggt gatcgccgac actggggtct
     1741 gtgcggagcg accaagcagc ttccacatag gaacgagcgc tgctgttgtc gccacgcaag
     1801 aagtagatcc agccgcgggt gtcccaatag gccgacatcc agaaaacata gttcagttgg
     1861 tccggggtca ggtgatcgag cggggcattc gatacattct gcaggcgcgt gttttcgatg
     1921 gtcaggcagg tgtctgacca tttttcggcc atgccgatct cgatattccg cttcgccagc
     1981 acatacgctg cgctgttgaa agtgttcggc gccgaagaga cgcttgtcgc ctgttccagt
     2041 tcggacaggc ccttagcccg gttgcccagg tcgagatcgc attcgccctc ggccaccaag
     2101 gtctcggcgt gatttggtgt gagagaaagc gctttctgga tctctggcat ggcttcgctg
     2161 caccgccgct gatcgcgcag catcatgcca aggttgcgat gggcgtagtg gtcgtcgggg
     2221 ttcacaagcg tttgcttgtt aaactgggct gcagcttctt cgtacttccg ctgccgccag
     2281 taaaccaacc ctaggttgtt ataagcgttt ctgctggctg ggtcattctt gatcgatgtt
     2341 tggaatgcct gcgcggcggc gtccagttcg ttcatagcca tcaacgcccg ccccaattcg
     2401 ttccatgcgc ttccgctctg cggcgtcaac gaagtgatct tgcgtagtaa gttcgctgcc
     2461 gtcgcgtagt ccccgcggct gtaagcctcg gtcgcgtccg agtacatatt tcgaaaatcg
     2521 tcgacagctt gtttttgctc ttgcaggttt ccggttggag gagcttgccg cggattgacg
     2581 ccaagccgaa agttgacctc caccgccaat tccacatcca caggattgcc ttgataagtg
     2641 ccgggatcga acttccattg gctcacagct tcgactgctg cctgatcgat gtccttgtcg
     2701 actcccttga ccacatgcac accgctaact gatccgtcct tgttcaccgt caatcccagg
     2761 gtaactatgc cttccttccg cttctcccgt gctttctcgg gataagaggg attcaccctg
     2821 ctcaacggtc tcggcgccgt cgcgcaaggc ccggccgacg caggattctt gtcgctgcac
     2881 acgtgcattg ccgaaacggc gtcaacggag atggtgtcct gagccgaaag gggactggtc
     2941 gagagcagca ggagaaacag aagacaaaga cggcgcatga ctatctacga cccgcctcat
     3001 tgtaactcac aaaagtccat gaatatgtac gcttagacgg cgcgggttat acgtgtatcc
     3061 gggcgggagt cggatgttct atctcgacgg catcaccacg ggacacgcct tacttttcca
     3121 gccccgctgc gaacgcatcc agcgacagcg agcgcagcag attccgccgc atccctcgct
     3181 ccacctccgc cagccggtcg gaagctgccg tgatccgctc aaaatccgcg gactccgcca
     3241 gacgcttcaa ctctgaagca atgtctgtgt tgcgcaccag gttcggcgcg ctggagtcaa
     3301 tgaacatcag atcgcgcagc aacgaataca gcgtgcggat caactcttcc gtcttctccc
     3361 gcccctcggc gcccggacga tacgactccg tcgccttgaa caactcgctg tgatcgccgc
     3421 cacgtaaagc cgagttcaga atcgtcagcg catgatcccg cgccacaatg tagcccgcca
     3481 gatcaaatcc acgcgcccgt cctaccgcgc cctgcgacag ccgcgccacc agcgcccgct
     3541 gcgccgcctt ccactccggc cgatgctcgg ccagatcctg ctcaatctcc tccgccgtca
     3601 gcgcttctaa cgtaaacacc atcg
//
LOCUS       DASPUL010000536         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_371738, whole genome shotgun sequence.
ACCESSION   DASPUL010000536 DASPUL010000000
VERSION     DASPUL010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371738"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VEJ00_12520"
     CDS             <1..1302
                     /locus_tag="VEJ00_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HXX02032.1"
                     /translation="DGISGQMVVNYFPQGGPSTKGFSTWLEMGNWYRALTDGRRDASP
                     EIKQQVNALTASAGTPLEKMKALAQFVQHDIRYVSIQLGIGSWQPHQAADIFVHRYGD
                     CKDKATLMSSMLNQIGIESYYVVINSERGSVGKDTPASIGAFDHVIVAIRLPENVSDP
                     SLIATVKHPKLGTLLYFDPTNELTPFGQIGGYLQANNGLLVAPDGGELVELPTQVAAL
                     NGIQRTGKLTLDAAGTLKGEVNETRMGDRAASERWRLRTVTKNTDRVKPIEDLLAGSL
                     SLFRITQASLVNLEETDRPFGFRYSFDAADYAKNAGGLLLVRPRVLGIRASGLLETKE
                     PRQFPVEFPGPARDSDTFDITIPDGYVVDDVPPPVDADYGFASYHAKTTVQGNVLHYT
                     RTFEVKELSVPVSRVDELKKFYRIIASDERNTAVLKAGASK"
     gene            complement(1382..2260)
                     /locus_tag="VEJ00_12525"
     CDS             complement(1382..2260)
                     /locus_tag="VEJ00_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02033.1"
                     /translation="MARFTSMRWMLALAAFALLAPGLAFAQDDPNDAPLGDVAREMRR
                     KSAASAGVIDNDNLPEVMDQAASRRPANSVVRFLLGGWKNSRPATPAVTCSLSFIANA
                     KTLPQGQYAEMELPASEVIKLEGPATMEGDALKVAVFNATEWHVSELAVALTIVRTDW
                     PRNALLPGDVALGDLTASSATKVPESGGGPPLEPEARSERKPDVTVIYRMRAPAAPFA
                     TTVFTAPLNQALAPDQEWHWAIVEARGYPPESYAGDAPRSAAQTADPALMQPSSAAAM
                     GRPQNPPAAVLSQNPR"
BASE COUNT          530 a          877 c          751 g          462 t
ORIGIN      
        1 gacgggatct ccgggcagat ggtagtcaac tattttccac aaggcggccc atcgaccaaa
       61 ggcttctcca cctggctgga gatgggcaac tggtaccgtg cccttacgga tgggcgacgt
      121 gacgcctccc cggaaatcaa gcagcaggta aatgcgctga ctgcttccgc cggcacgcca
      181 ctggagaaaa tgaaggccct cgcccagttc gtgcagcacg atatccgcta cgtttccata
      241 cagctcggaa tcgggagctg gcagcctcac caggcggccg acatcttcgt acaccgctat
      301 ggagactgca aggataaagc gacattgatg agctcgatgc tgaaccagat cgggatcgaa
      361 tcgtattacg tcgtcatcaa ttcggaacga ggatcggtcg gtaaggacac acccgcgagc
      421 atcggcgcct tcgaccacgt catcgtagcc attcggcttc ccgagaatgt ttctgacccc
      481 tcgctgatcg ctaccgtgaa gcatccgaag ctggggacgc ttctctactt tgatcccacc
      541 aacgaactga ctcccttcgg acagatcgga ggatatctcc aggcgaacaa cggtctgttg
      601 gtcgcgcccg acggaggaga actcgtcgaa ttgcccacgc aggtggcggc tctgaacggc
      661 atacagcgca ctggcaagct gaccctcgac gccgcgggca cgctgaaggg cgaggtgaac
      721 gaaacccgca tgggagatcg ggccgcttcg gaacgctggc gcctgcgcac cgtcaccaaa
      781 aacaccgacc gggtcaaacc gattgaggat ctgctggccg gatctctttc gctgtttcgc
      841 attacccagg ccagcctggt gaaccttgag gagaccgacc ggcccttcgg cttccgctat
      901 tcattcgacg ctgccgatta tgccaagaac gccggcggcc tcctgctggt tcgcccacgt
      961 gtgcttggca taagagccag cgggcttctc gagaccaaag agccccgcca gtttcccgtg
     1021 gagtttccgg ggcccgcgcg cgacagcgac accttcgaca tcaccatccc cgacggttac
     1081 gtcgtcgacg acgtgccgcc tccggtcgac gccgactacg gcttcgccag ctatcacgcc
     1141 aagaccacgg ttcaggggaa cgtgctccac tacactcgca ccttcgaggt gaaggaactg
     1201 agcgtccccg tcagccgcgt cgacgaactg aagaagttct accgcatcat tgccagcgat
     1261 gagcgcaaca ccgccgtctt gaaggctggc gcttcgaagt gaaatgctct cttcgcgcgg
     1321 gagcataaac tggggtcccc atcctttcgt gttttgtgcg aaagggtggg aagcacgggc
     1381 gctatcgcgg attttgtgac aggactgcgg ccgggggatt ctggggcctc cccatcgccg
     1441 cggctgaaga aggctgcatc agcgccgggt ctgcagtttg cgccgcgctc cgcggcgcgt
     1501 ccccggcgta actttccggt ggatatcctc tcgcctctac gatcgcccaa tgccactcct
     1561 ggtccggagc cagggcctga ttcagcggcg cggtgaacac cgtggtcgca aacggtgccg
     1621 ccggtgcccg cattctgtag atgaccgtaa cgtccggctt cctctctgaa cgagcttctg
     1681 gttccagcgg cggaccgccg ccactctccg gcaccttggt cgcactactg gccgtcagat
     1741 ccccgagtgc cacgtcgccg ggcagcaggg cgttgcgcgg ccagtccgtt ctcacgatgg
     1801 tcaacgcgac tgccagctcg ctgacatgcc attccgtcgc gttgaaaacc gcaaccttca
     1861 gcgcgtcgcc ttccatcgtg gccggccctt ccagcttgat cacttcgctg gccggaagct
     1921 ccatctccgc atactggccc tgcggcagtg ttttggcatt ggcaatgaac gacagactgc
     1981 aggttaccgc cggcgtggcc gggcgcgagt tcttccatcc tcccagcaga aagcgcacca
     2041 ccgagttcgc cggacgccgg ctcgcggcct gatccatcac ctccggcaga ttatcgttgt
     2101 cgatcacccc cgccgacgcc gcgctcttcc gccgcatttc gcgggccacg tctcccaggg
     2161 gcgcgtcatt cggatcatcc tgcgcaaacg cgagcccggg cgccagcagc gcaaacgccg
     2221 ccagcgccag catccaccgc atggaagtga atctcgccac gaaccctcaa ttcacggcag
     2281 ctcccgcccg aaggcagcgc ggaaccgccg aatgagccct gatccgattg tatggagaca
     2341 ggtcaccagg cgagagatga ccaaggtacc cggaacctca aacaggaagg aagcaatggg
     2401 cgcgcggacg aaatggagcg caacgggatg cgcgcccaag ctcagtcaga ccgttctaca
     2461 gcaacccctg cgaccgaaaa cgtgtcaccg gatcaacaac cgtcaccgct tcccgccgga
     2521 acaaccaacg ggccaccgca aagccaatcg ctgcatagca aagatgcatc attcgcataa
     2581 cacacccccg accggctggt cgatccacag ggggagaaac
//
LOCUS       DASPUL010000537         4040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_650944, whole genome shotgun sequence.
ACCESSION   DASPUL010000537 DASPUL010000000
VERSION     DASPUL010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650944"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VEJ00_12530"
     CDS             complement(<1..545)
                     /locus_tag="VEJ00_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXX02034.1"
                     /translation="MATITLPEADVKDTKTQETKKTTTKYVYFFGGGKADGDGKMKDV
                     LGGKGAGLAEMTNAGLPVPPGFTIQTEACREYMRGALSKEVDQQMNDALARLESLQGQ
                     KLGTGENPLLVSVRSGAKFSMPGMMDTILNLGLNDKSVEALARRSNNPRFAADSYRRL
                     IQMFGNVVLDIEKSAFDEVFEA"
     gene            complement(655..2817)
                     /gene="glyS"
                     /locus_tag="VEJ00_12535"
     CDS             complement(655..2817)
                     /gene="glyS"
                     /locus_tag="VEJ00_12535"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXX02035.1"
                     /translation="MTRLAMADFLLEIGCEEIPARMIVEAEQELTLRLTRLLGNERLK
                     YGSVSPFSTPRRIATQVTAVATSQPDVTEQITGPSVNVAYKDGQPTPAAHAFAKKAGL
                     DVSQLEKVSTPKGEYIAAKVTKKGRSAAEILAEALPKEIASIYWPKNMYWRKPSERFV
                     RPVRWLVAMLDGETIPLEFDGIRAGKTSRGHRILSDGEVTIPGAGPAYVDALRAAKVL
                     GRAEREQQIRKALDAATRTIPGARWREDKALLDTVVNLTEFPSVVLGGFDPQFLALPE
                     EVLVTVMRDHQKYFAVEDAQGKLLPHFLAVLNTDSDPQGLIRHGNERVLRARFNDARF
                     FWETDQKRSLLERLELLRHVTFQKDLGTYYAKTLRVQRLCSWLSEVLRQNGIAVRPGV
                     IHKAACLAKTDLTTELVKEFTELQGIVGGLYAREQQLDPDLPKATRLAVADTIYDHYK
                     PESTDDAVPRFTEGAVLSVGDKADTIAGMFALGMVPSGSKDPFALRRQANGIVKVIAE
                     KKFPFRLSDMMKDARAGYQKSEAEKKFVDDATFASSVAMFFRERLEFYLRELKEYEYD
                     VVKAVLAADADDVVDALARAEAVKQVLHLPEFQAIGAACKRMRNILRQAAEKGIEPAG
                     SVESPPDSAAEEKGLLDYLERAGAKVEEHRSRKEYLEALRLLATAREPVDRFFDKVMV
                     MVEDQRVRANRLALLRTLLKEFSTIADFSEIVTEGKAA"
     gene            complement(2874..3971)
                     /locus_tag="VEJ00_12540"
     CDS             complement(2874..3971)
                     /locus_tag="VEJ00_12540"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HXX02036.1"
                     /translation="MNSAKSNPLTFQELILRLQTFWAERGCVLQQPYDVEVGAGTMAP
                     ETFLRVLGPQPYKVAYVQPSRRPADGRYGENPNRLFKHMQLQVILKPPPENVQELYLE
                     SLSAIGIELRHHDIKFEEDNWEAPTLSAWGVGWQVMLDGLEITQFTYFQQCGGLDLFP
                     ISAELTYGLERIAAFLQDVDSIYDIVWARDPETGSVVKYGDVRIADEQQFSVYNFEVA
                     DVGKTWKHFELYEAECKELLRGYTDLEGKEKPHFSPNAREMGHPSTRVGQPLLTAEST
                     RFPLLAAYDLCLKCSHLFNILDARGAISVTERVGVIARVRALAVGIAKAWVSQQNDDQ
                     KSDKQSEQSDEPEPAQKKRKPKTETLSPVGG"
BASE COUNT          747 a         1366 c         1068 g          859 t
ORIGIN      
        1 gcttcaaaca cttcgtcgaa cgctgacttt tcgatgtcga gcaccacgtt gccgaacatc
       61 tgaatgagcc gccggtagga atccgccgca aatcgcggat tattgctgcg ccttgccagt
      121 gcctcgacgc tcttgtcgtt cagcccgaga ttcagaatgg tgtccatcat gcccggcatg
      181 gagaacttcg cgcccgagcg cacgctgacc agcaacggat tctctcctgt gcccagtttc
      241 tggccttgca gcgactccag cctggccagc gcgtcgttca tctgctggtc gacctctttc
      301 gacaaagctc cgcgcatgta ttcgcggcac gcctcggtct ggatggtgaa tcccggaggc
      361 accggcagcc cggcgttggt catttcggcc aggccggcac ccttgccgcc cagcacatct
      421 ttcattttgc cgtcgccgtc agccttgcca ccgccaaaaa agtagacata cttcgtggtc
      481 gtcttcttcg tttcctgcgt cttcgtatcc tttacgtccg cttcggggag agtgatggtc
      541 gccatatgag tccttttgta gccccgcagg ggcttgtgat agtagcccac cgcttcagcg
      601 gtgggggtcc agccagaatc acgagcaagt cccggaggga cggccgaaat gcgtctatgc
      661 cgccttccct tcggtcacga tctccgagaa atcggcaatc gtcgagaatt ccttcagcag
      721 cgtgcgcagc agtgccagcc ggttcgcccg cacacgctga tcctcgacca tgaccataac
      781 tttgtcgaag aagcggtcca ccggttcgcg agccgtcgcg agcaaccgca gagcttccag
      841 atactccttc cggctgcgat gctcctcgac cttcgcgccc gcccgctcca ggtaatccag
      901 caggcccttc tcttcggccg ccgagtcggg cggagactcg accgatcccg ccggttcgat
      961 tcccttctcc gccgcctgcc tcagaatatt ccgcatgcgc ttgcaagccg cgccgatggc
     1021 ctgaaactcc ggcaagtgca acacttgctt gacggcttct gctcgcgcca gagcatccac
     1081 cacatcgtcc gcatctgccg ccagcacggc cttcaccacg tcatattcgt attccttcag
     1141 ttcgcgcagg tagaattcca gccgctcgcg gaagaacatg gccacggaac tggcaaacgt
     1201 cgcatcgtca acgaacttct tctccgcctc ggacttctga tacccggctc gtgcatcttt
     1261 catcatgtcg ctcagccgga acgggaattt cttctccgcg atcaccttca caatcccgtt
     1321 cgcctgccgc cgcagcgcaa acggatcctt cgatccgctc ggcaccattc ccagcgcgaa
     1381 catcccggcg atcgtgtcgg ccttgtcgcc gacggaaagc acggctcctt cggtaaaccg
     1441 gggaactgcg tcgtcggtcg actcaggctt gtagtgatcg tagatcgtgt cggctacggc
     1501 cagccgcgtc gccttcggca agtccggatc aagctgctgc tcccgcgcgt acagtccgcc
     1561 cacaattccc tgcagctcgg tgaactcctt caccagttcc gtcgtcagat cggttttcgc
     1621 caggcaagcg gctttgtgaa ttacgccggg acgcaccgcg atgccgtttt gccggagaac
     1681 ctcgctcagc caactgcaca gccgctgcac gcggagcgtc ttcgcgtaat aggtgcccag
     1741 atctttctgg aaagtaacgt ggcgcagcag ttccaggcgc tccagcagcg acctcttctg
     1801 atccgtctcc cagaagaagc gcgcatcgtt gaagcgcgcc cgcagcaccc gttcgttccc
     1861 gtgccggatc agcccctgcg gatcgctgtc ggtattcagc accgcaagga aatgcggcag
     1921 cagcttgccc tgcgcgtctt ccacggcaaa gtacttctgg tggtcgcgca tcactgtgac
     1981 cagcacttcc tccggcagcg cgagaaattg cggatcgaac ccgcccagca cgaccgacgg
     2041 aaactcagta aggtttacca ccgtgtcgag cagcgccttg tcttcgcgcc agcgcgcgcc
     2101 ggggatggtt cgcgtcgcgg catcaagcgc cttgcgaatc tgttgctcgc gctctgctcg
     2161 tcccaaaact ttcgccgcgc gcagcgcatc cacgtatgcc ggtccagcac ctggaatcgt
     2221 cacctcgcca tccgacaaaa tgcgatgccc acgcgaagtc ttccccgccc ggatgccgtc
     2281 gaactccagc ggaatcgttt ctccgtcgag catggccacc agccagcgca ccggacgcac
     2341 aaaacgttcg ctgggcttgc gccagtacat gttcttcggc caataaatcg acgcgatttc
     2401 tttggggagc gcctccgcca gaatttccgc cgcgctgcga cccttcttcg taaccttggc
     2461 ggcgatgtac tcgcccttgg gcgtcgaaac tttttccagc tgggaaacat cgagcccggc
     2521 cttcttggcg aacgcgtgcg ccgccggcgt cggctggcca tccttatagg ccacgtttac
     2581 ggagggcccg gtaatttgct cggtaacgtc gggctgcgag gttgccactg cggtcacctg
     2641 cgtggcgatc cgtcgcggag tagaaaatgg gctaacgctg ccgtatttca acctctcatt
     2701 gcccagcaat ctggtaaggc gcaacgtgag ctcctgctcg gcctctacaa tcatccgcgc
     2761 cggaatttct tcgcacccga tttctagcag gaaatccgcc atagccaacc gtgtcatcct
     2821 gagcgcagca aaacgatttg cggagcgaat cgttttgcgg agtcgaacga tccctaccca
     2881 cctaccggtg acagagtctc cgtcttcggt ttccttttct tctgagccgg ttcaggctca
     2941 tcggactgtt ccgactgctt gtcgctcttc tgatcgtcat tctgctgcga tacccacgcc
     3001 ttcgcgatcc ctaccgccag tgcccgcacg cgcgcaatca cgcccacacg ctcggttacc
     3061 gaaatcgctc cccgcgcgtc aagaatattg aagagatgcg agcatttcag gcagagatca
     3121 taagctgcca gcaggggaaa acgtgttgac tcggcagtca ggagcggttg ccccaccctt
     3181 gtcgacgggt gccccatttc tcgcgcattt ggcgagaagt ggggcttttc ctttccttca
     3241 aggtctgtat acccccgcag caattccttg cactccgcct catacagctc gaaatgcttc
     3301 caggtcttcc cgacatccgc cacttcaaag ttgtacaccg aaaactgctg ctcatccgct
     3361 atccgcacgt cgccatactt cacgacactt cccgtttcgg gatcgcgtgc ccacacgatg
     3421 tcgtagatcg aatccacatc ctgcaggaaa gccgcgatgc gctccagccc gtacgtcagt
     3481 tcggccgaaa tcggaaacag gtccagcccg ccgcactgct ggaagtaggt gaactgcgtg
     3541 atctccagcc cgtcgagcat aacctgccag cctacgcccc acgcgcttag cgtcggcgct
     3601 tcccagttgt cctcttcaaa cttgatgtcg tgatgccgca gctcaatgcc gatcgccgat
     3661 agcgactcca ggtacagttc ctgcacattc tccggcggag gcttcaggat cacctgcagc
     3721 tgcatgtgct tgaacagccg gttgggattc tcgccgtagc gcccgtccgc cggccgccgc
     3781 gacggctgca catacgccac cttgtacggc tgcggcccca gcacgcgcag aaacgtctcc
     3841 ggagccatgg tcccggcccc gacttccacg tcataaggct gctgcagcac gcagccgcgc
     3901 tccgcccaga acgtctgcag gcgaaggata agttcctgaa acgtgagcgg gttggacttg
     3961 gcagaattca caaatatttt gctcgcgaag gaataatctg tgattgtaac ggatggtcga
     4021 gtcagccgtc agctacgcca
//
LOCUS       DASPUL010000538        19909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1325732, whole genome shotgun sequence.
ACCESSION   DASPUL010000538 DASPUL010000000
VERSION     DASPUL010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 19909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19909)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19909
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325732"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..1386
                     /locus_tag="VEJ00_12545"
                     /pseudo
     CDS             317..1386
                     /locus_tag="VEJ00_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611760.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2105..3031
                     /locus_tag="VEJ00_12550"
     CDS             2105..3031
                     /locus_tag="VEJ00_12550"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HXX02037.1"
                     /translation="MTALESRFSEAEPNLRSSRKRLIREILDNPEDTYFLSSRALAKH
                     YGLDPTTIVRTVQALGYKRYAEFAADLRSHFVSRITPYALMKSTMREKRSVAQHVEHT
                     LQMDLHNVNALCSTLDVSRVLETAKRIDRARKIIVVGVDFAAALSQLLAYALVSIGYD
                     ARAPIGSSGNLCQSILLLEPRDLLIAFSFGRCLQDTVDSVLLAHRNGVPTFGITDSEK
                     SPIARYCDSSLLVSIASPSFHGSYVAVVSVVNALLMACTQIHPRRTLAVLERKEKESG
                     SRWYAPPVNRSENRDQRKIDRSLRTGRLLVRT"
     gene            3440..4516
                     /locus_tag="VEJ00_12555"
     CDS             3440..4516
                     /locus_tag="VEJ00_12555"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HXX02038.1"
                     /translation="MEIVPYSLEQKVVFHVALPAVPTSEGLLLRLRTEEGVVGWGEAS
                     PYSPVTSETPQSDMTMASSLAALIRNRDAFSIAKTVADMDALSPFNTSIKAAFEIALW
                     DICGKIAGQPVCCLLGKFRESFETDKTVFIDTPQAMAETAREYVRDGFRVVKVKVGES
                     PEKDVERLRAVREAVGNDIGIRIDANQGWSPTDAVRSLRALEKYQIQDCEQPTPYWDW
                     QGLKYVRDHSPIPVMADESVHSPHDAIEAARLEACDMINIKLMKSGGILNATRIAHIA
                     DAAGMSCMLGCMAETSLGLTAAAHVVASQGNIVYADLDSSLFVVNNPIAGGMKIDKGT
                     VYLGPEPGLGLEVDPLFVKKLRPV"
     gene            4849..8286
                     /locus_tag="VEJ00_12560"
     CDS             4849..8286
                     /locus_tag="VEJ00_12560"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXX02039.1"
                     /translation="MKSSLIRFIEILLVLPWLAGTGLGQSASTGAITGTATDPSGAVV
                     VGADITVTNEATGGVLNSSSSTNGSYSVPLLAPGLYTVKASKTGFKAVVRAHVLVTVT
                     ETARVDVRLEVGAPSEVITVSAEVPLLNTIDSTEGALTDSRSIESAPLATRNYTQIIG
                     LSPGISSQVTDASSLGGGSGSQAAGTNGFSAHGGATNDNNYQINGTEVNDLMGSGSLS
                     GGVAVPNPDTIQEFKVQTGQYDASYGRNGGANVDLVTKSGSNQIHGSLFEFLRNDVLD
                     ANNYFLNQERQPRAAFKQNQFGGSLGGPIVRGKLFYFGSYQGTRQRNGLGNGQCISKV
                     FLPPFTNDRSEAALGALFSGPTGGAIKPDGSNISPEALALLNLKLPNGSFLIPTPQTV
                     TPVSNPTLTQLSLEGSSSFSLACPYAENQFLANADYIQSDKSTLSGSFFFSNTSQTTT
                     LSTNPNIAGNGVPGSPSSQTNDYRVFTLSHRYAFSSNVVNQATLGYHRVLGILAQTEP
                     FTYSDLGVTAPAFDNGFPEIGISGSFETGGDGQGINVAQNLYNFGEGLFWNHGAHSIH
                     VGGGVERSQVNQTGFHFFGGTQFPDYGQFLLGNAQLALDVPGLFNRYYRTWDGNAYVQ
                     DNIKLARTFTLDLGFRYERLGDLSDKLGRDSSFDVAAADHTGAGSTAGYVVSSNYSGT
                     LPAGVERASNEAATRGLGQNTLGPRVGFSWQLPGNDHVVLRGGYGIYYSRTTAQPVFQ
                     ELTSPPFGQIRITQGVAIPFSNPFPAAPALPSFPTYSLATCPLDILPCVSFANVSINV
                     KPPATQQYNLNAQVALAKDFALEIGYQGARGTRLLEFRNFNQAFLASAAEPVNGATTN
                     TFFNVTQRTPVPGISTSAFQITSTGASWYDAMVASLNKRFSKGLQFLASYTWSSDLAT
                     TQTSTTSTGTGGIAIGDQNNARQRYGWDNFVRPQRLVLSWVYEFPNFTGHNAFMHSAL
                     GGWSVAGVTTIQSGQRLTATATNIQNVVGTFSDRAPASAAGCNGKFVNSGSVQSKLKN
                     YIDASCFDLSNYPVVGADGIGTGFGTSGIGEIIGPDQNNWDLSVAKNTGIGERLRVQF
                     RADFFNAFNHPQFANPDVNLGEAAVQLGLVAPNSGFGTITATSTNPRLLQFSLRLVF"
     gene            8415..9206
                     /locus_tag="VEJ00_12565"
     CDS             8415..9206
                     /locus_tag="VEJ00_12565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX02040.1"
                     /translation="MGLDRKEVTIRELSAHNEMRACIDLQKRVWRGSDLDVVPQNIFV
                     VACRTGGLLLGAFERDSLVGFALAFPAVRHGHIYLHSHMTAVAPELQGHGIGKRLKLA
                     QREHSLARNFDLIEWTFDPLQPGNANFNIAHLGAIVREYLPNFYGNTTSALDAGLPTD
                     RLVAEWWIREPRVEQILEGRPQPATHVRRARVPRRIREICAANADAARRIQQELRQEL
                     EELFRTSFVITGFETDADYATYLLECYENRDDYAARASHASQIAL"
     gene            9184..10284
                     /gene="menC"
                     /locus_tag="VEJ00_12570"
     CDS             9184..10284
                     /gene="menC"
                     /locus_tag="VEJ00_12570"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HXX02041.1"
                     /translation="MRLKSPFETSFGVTQDRRILLVEAVADGVSGWGEITSLESPLFN
                     SETTDTAWHIVSDYLAPALVGKEVAAASELPALLAPFRGHEMAKAGVENALWDAEAQQ
                     KRVPLHALLGGTRPEIACGVSLGIRETPEQLVSKVEQELRDGYQRIKLKIKPTKDLEF
                     VAAVRKEFPETLLSVDANSAYILSDAAHLQKLDAFNLLMIEQPLQWDEIHEHARLQAL
                     LRTPICLDECITNARHAQSAIELGACRIINIKLGRVGGHTSARCIQDICLQRGIPAWS
                     GGMMESGIGRAHNIAMSTQQGFTLPGDVSASRRYWAEDIIEPEVEVSPNGTIRAPQSP
                     GLGFAVKRGLVERLTSRSRSWSAKKDLVSVGD"
     gene            10285..12042
                     /locus_tag="VEJ00_12575"
     CDS             10285..12042
                     /locus_tag="VEJ00_12575"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HXX02042.1"
                     /translation="MLKRNLHSVRSAAQWRLGVWIATASLSASVIWAPTLLAQTASVA
                     GPGYDVIIRNGRIIDGTGNPWVSGDVAIQGERIAAIGSLAAATARRTIDASGLIVSPG
                     FIDMLGQSEMALLIDNRSLSKLSQGITTEITGEGDSIAPQNATTLASLAPELDRYQLK
                     VDWTTLDGYFVRLEKSGTPLNFGTYVGAAQVREAVLGSANRAPSPEELQKMKDLVAQA
                     MQQGAFGISTALIYPPGHYAKTDELVELAKVVGQYGGIYATHMRSEGKFEVRAIDEAL
                     QIGREAHVPVEIFHLKVIGESRWGSMPKIVAKIQAARDSGQDVSADMYPYVAGGTALA
                     ASLPPWVADGGRTQLLERLRDPATRSRIKEELKTEHDDWENLFLDSGGGRGVLIAGIE
                     KPALERYDGKTIAEIAEMEKKSPVDTLLDLVVEDEARTGAIYFIASEDDLQYGLKQPW
                     TSIGLDANESSPDGPLFEVHDHPRAYGSMPRFLGHYVRDQHLLPLEQAIRKITSLPAQ
                     RERLRDRGLLKVGYFADITLFDPATIVDHATYENPVQLSEGVKYVFVNGQLEFENGRP
                     TGAAAGQVLRGPAWGQERK"
     gene            12344..14173
                     /locus_tag="VEJ00_12580"
     CDS             12344..14173
                     /locus_tag="VEJ00_12580"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXX02043.1"
                     /translation="MNADDLGAFLDGIVPQELARENIAGAVVAVVKDGKVLFAKGYGY
                     SDVKKKTPVSATDTLFRPGSISKLFTWTAVMQLVEQGKLDLDRDVNDYLDFKIPPTYS
                     KPITLRNIMTHTAGFEETAQELFVGSAKDLKPLGTYLREHLPERIFPPGTTPAYSNYA
                     TTLAGYIVQRVSGQDYFDYIDEHILKLLGMTRTTFRQPLPDPLAPMMSKGYSLATDPA
                     KDFEYVEAAPAGSSSTTATDMTHFMLAHLQEGKYEGAQILRPETVQMMHSRQFENLPS
                     MNAMCLGFYEETRNGHRIIGHGGDTEYFHSDLHLIPDENLGFFVSYNSAGKGDIRPRE
                     ALWHAFLDRYFPVQLPEIPHLATAAQDADAVSGHYLSSRRPETTILRILAVTGEAKVY
                     RNDDGTISVDELKDMSGVPIKFGETGPMLFRKVNGQEQLGFKRDYSGNTVGAIDFPFM
                     LLQKASWWDNSALQLVLIVGSLSVLVLTILLWPILAAVRWHYAKPLNLTPEQRRLRLL
                     VRLAGVALVVFVAAFAAFFTAGEKHIEILSPKGNPWLRLIQLVGWLGVLGSLIAIYNA
                     VKSWQDRVRWLWSRVGDTLIALACIGTVCFVFTCHMLHWSLRY"
     gene            complement(14278..15852)
                     /locus_tag="VEJ00_12585"
     CDS             complement(14278..15852)
                     /locus_tag="VEJ00_12585"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXX02044.1"
                     /translation="MAKRLSELLLAWVAVVPLFAQAGPPPDLDGYISRAMQTFEVPGL
                     SIAIVKDGRVVLAKGYGVRKMGESVPVDENTLFGIGSNTKAFTSALLASLVDEGKLSW
                     DDRVYERLPGFQMYDPYVSHELTIRDLLTHKSGMGLGEGDLLFWPHSTYSRDEIIYRL
                     RFMKPKSSFRSRFAYDNLMYIAAGQIVAAVTGKSWEDNIRERIFAPLGMSSSNLTNSA
                     WKPGDNYGWPHSKLPGTMQLIDFVPLDNAAPAGAINSSAAEMARWLLLQLNHGRLPGS
                     DRRLFGEQQSKEMWSPQTILPIADVPPALAALKPNFADYGLGWFLNDYHGYKLVGHSG
                     GVAGFVSRVQLVPDLRLGIVILTNTESGSANNSILYHILDYYLSLPPTDWITPYRKAE
                     LAREKDAAEVMAKQAASRAAGLAPPLPLDKYAGVYSDDWYGTSTIKLVGGKLVFTLDH
                     TPNAIGDLEYWQYDTFKAHWRDRKIEDAFLTFALRPDGTIDHFTMSAVSPLADFSFDY
                     QDLLFVPLASAPPRQQ"
     gene            16072..17406
                     /locus_tag="VEJ00_12590"
     CDS             16072..17406
                     /locus_tag="VEJ00_12590"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXX02045.1"
                     /translation="MPLDSGKSLLARVLRFRDLVVLVIGTVIGSGIFLVPGAILRQVG
                     NSVPLATAVWLIGGLLSLLGALTCGELSAMKPQAGGVYVYIRDCFGPFVAFLFGWTLF
                     FVISSGSIATLAVAFCDYSAELVPLNPLMGRLVSVVMITVIALVNVLGTRRSADLQDV
                     TTAVKVAAIVLMASALLGLGKGSSSVPVAPHDLSAGGYASGFGLAMISVLWAYEGWQY
                     ATFSAGEAIDPQRNFPLAFFAGSAALIAIYLLANFGYLAALGPAGVSASDRVAAAAVS
                     RVIGPAAAKLVALTILVSIFSATNGIVLTASRVYYAMAQDGLFFKRLAEVHQRFKTPA
                     FAVVACCAWSAVLAVTGTFEQLLTYVVFIGWIFYALAAGTVFVYRKREPEADRPYRVP
                     GYPFTPALFIAAAAYLVINTVVTQPRRAAAGIAIVLLGAPAYAIWRRKERTS"
     gene            complement(17597..17851)
                     /locus_tag="VEJ00_12595"
     CDS             complement(17597..17851)
                     /locus_tag="VEJ00_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02046.1"
                     /translation="MLWWPDRQSDRADLRRLLDAIRGACGKVIMTSRSIEDWLKPSQR
                     FQVLLGGLDGEERQEYCEVILRELVLRVNRERLSTESLRT"
     gene            complement(18350..19600)
                     /locus_tag="VEJ00_12600"
     CDS             complement(18350..19600)
                     /locus_tag="VEJ00_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXX02047.1"
                     /translation="MPRKPNIKLYLRFRTPDGKQSPYCPAVCDSKSRLRDFWCLVNGQ
                     PEFHPNGYYYQRVKRDGKEKWESLGTDPHTASCKAKAQAKQEAHGTEDVLGLKTSQPE
                     QPAAAEKAPGYRVTDEVREYLINAAKLAAKTYKAYAKSLELFQESCKKTYMHQIGKQD
                     LQALDTFLVQRGNDDRTRSNRVGHIVTFLRNEQGRRPGPPITDVTITIKYVESPPEAY
                     TRELEDLFQVSDEDERFLWRFFLGTGFRESETAVAESTDVNHDTKMIRVDQKPEFGFK
                     PKDCEKRWVPIPDALIAEIDARAKSGSCSLLFDNNGRPDGHILRRLKRVAFDGGLNCG
                     KCKGTSDGKVVSCGDGPVCEKWILHRFRKNFATDRHNAGASARKIQKWLGHADLETTL
                     RYLALGDDTTDEVREIVNGVHIGL"
BASE COUNT         4186 a         5640 c         5890 g         4193 t
ORIGIN      
        1 gccggtggaa actgcgattg cggcgacgac tcgcgcgttt aagtcgatca ccaccgtgcg
       61 cactcagcgc taggctagac cgcatcttgg cgttcctttg tgcagaaccg cttcttgttg
      121 atcgatgaca agtaaatgac actgcaacgc aattgccccg agacgcgacg gaccagaaag
      181 tcgcatgtcg ctcgacgaca aaagagttgc tagatgtttt gtacggagaa acggcgagga
      241 ttgacccaaa acccaaaaac tgacacaagg gagattagag gtaagctggg gcaggagtcc
      301 ggaggagacg gggacgatgc ccaggaaggc gcatacggaa gagcagatcg tagcggtact
      361 gcggcaggtg gaagcaggag cgcgcgtgga cgacgtttgc cgcaaggtgg gaatcagcca
      421 ggccacttac tatctctgga aacgaaaata tacgggagtt ggggtgagcg agttgcgtga
      481 gctacggcag ctccgcgaag aagtgggacg actcaagcgc ttggtggcag acttgagcct
      541 ggatcggcag atcctgcagg agatcgtgtc aaaaaagctg tgaggcctcg tgcgcgtcgg
      601 aagttggcgc gctgggcgca agaggcctat cgaatcagcg agcagcacgc ggcgcggatc
      661 gtgaagttgg cgatcgggac actgcggtac gagagccgga aggtgttcga tgaagtgttg
      721 cgacaccgct tgcgagagtt ggccggcatg catgtgcgct acggttatcg acgactgacg
      781 gtgctgctgc gacgcgaggg ctggcacgtg aatgcgaagc gcatttatcg gctgtaccgg
      841 gaagaagagt tgattgtgcg caccaagcaa cgaaggaaga tagcgcgacg acggccggtc
      901 gcaagcggca tcgccgcgcg accgaaccag tgttggagta tggactttgt gagcgataag
      961 ctggcggatg gacgttcgtt ccgcattctg acagcggtgg accagttcac acgggagtgt
     1021 gtggcgttgg aagccgaccg ttcgatgact ggaatgaaag tgtcgcaagc gctggacgcg
     1081 gcaaatcagg aacgaggttg cttgccggaa agcataacgg tggacaacgg gagtgagttt
     1141 tgcagtcggg cgctggaagc ttgggcgatg agtcgcaaag tacagttgcg tttcattcgg
     1201 ccaggccgac ccgtggagaa cggattcatc gagagtttca acggtcggct gcgggacgag
     1261 tgtttgaacg tagagtggtt cgcatcgctg gaagatgcac ggcggaagct ggcgaagttc
     1321 cgcgagcatt acaaccacga gcgtccgcac agtgcgctgg cggatcggac gcccgcagcg
     1381 ttcgcggagc tgcaccgaga gaaagctagt acattgctgg ggcgcgcact ccagagtctc
     1441 ttgatggagc ctctggagtg cgcgaaaccc acgtcaacag cgggagaaag gacttacact
     1501 gcccaaaacc tctagcctcg tttgtgacag aaaatgggag cggtccacaa acatcaacct
     1561 tcgtattcag gaccgtgcgg agtaaggggg caggtcatag ccgtgctttg atggcgcggg
     1621 atgcgtcagt tgtgtacctt ctgtagcgag ccaaggagga aactctatgg ctcgcaaagc
     1681 tggccaaatc atggcacgac gcgaaccgca tcagtcagca tgggccgcga cgagggcgag
     1741 ccttggagtt cacatgcccg cgctgcggaa tcgactttct cgtccatgaa gatggattgg
     1801 tttttcggtc ggtgctgaag tagtggctct tggcggaggt tcagactgca taaccgttcg
     1861 gtgacatgca agcgtcaaag aagcagtcgg ctcagacttt gcattgggaa gtcgccgttt
     1921 cggagcgatt tagaaatttc gagcttcggt ggttgagcta gaatcaaccc gcctgttgct
     1981 gtctttagct attggcccaa aagctgcact gattggagcg cacaagattc gaggatgagg
     2041 cagagctatg tcgggaaacg ataacaagac gaacaagagt caaactcccg agcgaagggt
     2101 ctggatgacc gctttggaaa gccgctttag tgaggccgaa cctaatctga gatcgagccg
     2161 gaaaaggctg attcgcgaga ttctcgacaa tccagaagac acttactttc tgtcctctcg
     2221 cgctttagcc aaacattacg gcctcgatcc caccacaatc gtccgtactg tccaggctct
     2281 tggctataag cggtatgctg aattcgccgc ggatctgcga tcgcactttg tgtcgcgaat
     2341 cacgccctac gctttgatga aatccacgat gcgcgagaag cgcagcgtcg ctcagcatgt
     2401 tgaacacact ctccagatgg atctgcataa tgtgaacgcg ctgtgttcga ccctggacgt
     2461 gagccgcgtt ctggaaaccg caaagcggat tgatcgtgct cgaaaaatca tagtcgtggg
     2521 ggttgatttt gccgcagccc tttcgcaact cttagcgtac gcgctggttt cgatcggcta
     2581 tgatgcacga gcgccgattg ggtcctccgg aaacttgtgc cagagcattc ttctgttaga
     2641 gcctcgggat ctgctcatcg cgtttagctt tggtcgttgc ctccaagata cagtcgattc
     2701 agttttgttg gcgcaccgga acggcgttcc cacgttcggg atcaccgata gcgagaaaag
     2761 cccgatagcg cgctattgcg attcttccct tctagtgtct attgccagcc cctcattcca
     2821 cggttcatac gttgctgtgg tttcggtggt caatgcgctg ttgatggcct gcacgcagat
     2881 ccatcctcgc cgtacactcg cagtgctaga gcgcaaggag aaagagtctg gatcgcgttg
     2941 gtacgctcca ccggtcaaca ggtctgagaa tcgagatcag cgcaaaatcg accgatcgct
     3001 aagaacgggc agattgctcg ttcgcacgtg acaactaagt cgctctcgtc ggcgatgtcc
     3061 atcgtcgact ggaggtacgt ccgccgacgg caacggcctt ccgggcccca ggaatcgttc
     3121 cgcgattaag aaaatctgcc ccgagggacg ctcgaaacag gccatttggc gctgtcttga
     3181 taagcactat atggtgcaat acatccagta gtgtcatcat tcagtgaaga aagatgttga
     3241 caaagaattc ccatcgcgat atgaagggtc gttctcctgg agtcgaggct tgcctgtcat
     3301 gcagactact cggagcggtt ttctaaagag cgtagctggg atggcgattg ctggcgctgt
     3361 tctgaggcga cccacggatg cgttgttata cgcacaggaa aagggtcgtg tctccccgcg
     3421 gtccgcgcga atccgcgacg tggagatcgt tccgtacagt cttgagcaga aggtcgtgtt
     3481 ccacgttgca ttgccggcgg tgccgacctc tgaaggcctc ttgctgcgcc tgcgaaccga
     3541 ggagggtgtt gtcggttggg gcgaggcgtc tccctattct cccgtgacca gtgaaactcc
     3601 gcagagcgac atgaccatgg ccagcagcct ggctgcattg attcgcaatc gtgacgcttt
     3661 cagtattgcc aagaccgtcg cggacatgga tgcccttagc cccttcaaca ccagtatcaa
     3721 ggcggcgttc gaaattgcgc tttgggacat atgcggcaag attgcagggc agccggtgtg
     3781 ctgtctgtta ggaaagtttc gcgagagctt cgaaaccgat aaaaccgttt tcatcgatac
     3841 tccccaagca atggcggaga cggcacggga gtatgtgcgc gacggcttcc gagtagtcaa
     3901 ggtcaaggtc ggtgaatcgc cagagaaaga cgtcgaaaga ttgcgtgccg tccgcgaggc
     3961 tgtgggcaac gacatcggca tacgaatcga tgcgaaccag gggtggtcgc caaccgatgc
     4021 ggttcgcagc cttcgcgcgc tcgagaagta ccagatccag gattgcgaac agccgacgcc
     4081 gtattgggat tggcaaggcc taaaatacgt tcgcgatcat tcgcccatcc cggtaatggc
     4141 ggacgaatca gtgcactccc cgcacgatgc aatcgaggcc gcccgcctcg aagcctgcga
     4201 catgatcaat atcaagctca tgaaaagcgg gggcattctc aacgcgacac gcattgcgca
     4261 tatcgccgac gccgcaggca tgagctgcat gcttggctgc atggctgaaa ccagccttgg
     4321 tcttaccgcg gctgcccatg tggtcgcgtc gcaagggaac atcgtatacg cggatctgga
     4381 ctcatccctc tttgttgtga acaacccgat tgctggcggc atgaagatcg acaagggaac
     4441 cgtgtatttg gggccagagc cgggattggg cctggaagtg gaccccttgt tcgtcaagaa
     4501 attgcgcccc gtttagcgaa ctgggagatg gaacgcgacc gtaacgcgac cgcttccaag
     4561 gccgtggctt acagcgggag ctgaatgcag cacatgatgc atcagtcagg attgtggcat
     4621 caaaatatga aattgtgctt gacaaggcaa cgcacttagt catacttctt cgcccgagtg
     4681 cggatgcgcg gcatcctcca gctccatagc tggaacgatt gcccgggagt gcaggaactc
     4741 cacgcactcg atcacatgac gggaaggcgg aagagggagc gtcgcacctg tcgcaatcag
     4801 ttccgtatgc cgccaatggc ggaaatgcgt ttcacaggag ggccactcgt gaaatcgtcg
     4861 ctcatccgtt tcatcgaaat actgctcgtg ctgccatggc tggctggaac cgggctgggg
     4921 caaagcgcga gtactggcgc catcactggg accgcgactg acccttcggg agcggtcgtt
     4981 gtcggcgccg acattaccgt gaccaatgaa gccacgggag gagtgctcaa cagttcgtcc
     5041 tcgacgaacg gaagctattc tgtccctctc ctggctccgg ggctctatac ggtcaaggcg
     5101 tcgaagacag gcttcaaagc agtggtgcgc gcccacgtgc tggtcacggt cactgaaacg
     5161 gcccgcgtgg acgtgcgctt agaggtcggc gcgccgtcgg aggtgatcac cgtcagcgcg
     5221 gaagtccctc ttctcaatac catcgattcc acggaagggg cgcttaccga tagccgatcc
     5281 atcgagtcgg cgccgctggc cacgaggaat tacacgcaga tcatcgggct gtcgccggga
     5341 atctcgtcgc aagtcaccga cgccagttca ctgggcggcg ggtcgggaag ccaagccgct
     5401 ggaaccaatg gcttctccgc ccatggaggc gcgaccaacg ataacaacta ccagatcaac
     5461 ggcacggaag tgaacgatct catgggctcg ggatcgctca gcggaggagt tgcagtgccg
     5521 aatccggaca ccatccagga gttcaaggtc cagaccggtc aatatgacgc ctcatacgga
     5581 cgaaatggag gcgcgaatgt cgatctggtg acgaaaagcg ggagcaatca gatccacggc
     5641 tcgctcttcg agtttcttcg caacgacgtg ctggacgcca acaactactt cctgaaccag
     5701 gagcgccagc cccgcgcggc attcaaacag aaccagtttg ggggcagcct gggcgggcct
     5761 atcgtgcgcg gcaaactctt ttactttggt tcctaccagg gaacgcgaca gcgaaatgga
     5821 ctgggcaatg gtcagtgcat tagcaaagtg ttccttcccc cgttcacgaa cgaccgctcc
     5881 gaggctgcgc tgggagcgct gttctccggt ccaactggag gagccatcaa gccagatggc
     5941 tccaacataa gtcctgaggc gctggcgctt ctgaatctca aactcccgaa cggcagcttc
     6001 ctaattccga ctccgcagac ggttaccccc gtttcaaatc cgacgctgac gcaactttcc
     6061 ctggaaggca gctcgagctt cagtctcgcc tgcccctatg cggaaaacca atttctggcg
     6121 aacgcagact atattcagag cgataaaagc accttgtcgg gaagtttctt cttttccaac
     6181 accagccaga caacgacgct cagtaccaac ccaaacatcg ctggaaacgg cgttccgggg
     6241 tcgccgtcaa gccagacgaa tgattatcga gtttttaccc tttcccaccg atatgccttc
     6301 tcttccaatg tggtgaatca ggccacgttg ggttatcacc gcgtgctcgg gattctggcg
     6361 cagacagagc cattcacata ctccgacctt ggggtgaccg ccccagcgtt cgacaacggc
     6421 tttccggaga tcggaatttc cggttcgttc gagacgggcg gggacgggca gggcatcaac
     6481 gttgctcaga acttgtacaa cttcggcgaa ggcctgttct ggaatcacgg ggcgcactcc
     6541 attcacgtgg gtggaggagt cgaacgttcc caagtcaacc agaccgggtt tcatttcttt
     6601 ggcgggacgc aattcccaga ctacgggcag tttctgctgg gaaacgcgca gcttgccctt
     6661 gatgttcccg gtttgtttaa ccgctactat cggacctggg acggcaatgc atacgtccag
     6721 gacaacatta agctcgcccg cactttcacg ttggacctgg gctttcgtta tgaacggctc
     6781 ggcgatcttt ccgacaaact ggggagggac tctagtttcg atgtcgcggc ggcagaccat
     6841 accggggctg gcagcacggc agggtatgtc gtgtcgtcga attatagtgg gacccttcct
     6901 gcgggcgtgg agcgcgcctc caacgaggcc gcaacccgcg gactgggcca gaacactctg
     6961 gggccgcgcg tgggcttctc ctggcagttg cctggcaatg atcatgttgt gctgcgcggt
     7021 ggctatggca tctactactc gcgcacgact gcgcagccgg ttttccagga actaacctct
     7081 cctccgttcg ggcaaatacg gatcacacaa ggcgttgcga ttccgttctc gaacccgttt
     7141 cctgctgcgc ccgctttgcc ctcttttccc acctattcgc tggcgacgtg ccccttggat
     7201 atattgccct gcgtttcgtt tgcgaatgtt tctatcaacg tgaagcctcc tgcgacccag
     7261 caatataacc tgaacgcgca ggttgcattg gccaaggact ttgcgttaga gatcggatac
     7321 cagggggcga ggggaactcg actcctggag tttcgcaact tcaatcaggc attcctggca
     7381 agcgcagcgg agccggtcaa tggagcgacg accaatacgt tcttcaatgt gacgcagagg
     7441 acgccagttc cgggcatctc gacgtcggca ttccagatca cctccaccgg cgcctcctgg
     7501 tacgacgcga tggtagccag cctgaacaag cgcttcagca agggcttgca gtttttggct
     7561 tcttacacat ggtccagcga tctggcgacc actcagactt ctacaaccag taccggtacg
     7621 ggcggtattg cgatcggcga tcaaaacaat gcccgccaga ggtatggatg ggacaatttt
     7681 gtccgcccgc agcgtctagt cctcagctgg gtctatgagt ttccaaactt taccggtcac
     7741 aacgctttca tgcatagcgc tttaggaggg tggtctgtag ccggagttac aacgatccag
     7801 tcgggacagc gccttaccgc tacggccacc aatatacaaa acgtggtggg gacgttctct
     7861 gatcgcgcgc cagcctcagc agccggctgc aacggcaagt tcgtcaactc cggctcggtg
     7921 caaagtaagc tgaaaaacta catcgacgca tcctgtttcg atctgagcaa ctatcccgtg
     7981 gtaggggccg acggaatcgg gacgggattc ggcaccagcg gcatcgggga aatcatcgga
     8041 cctgatcaaa acaactggga cctcagcgtg gccaagaaca cagggatcgg cgagcggctt
     8101 cgcgttcaat tccgcgccga tttcttcaat gcgttcaatc atccccaatt cgccaatccc
     8161 gacgtcaacc tcggagaggc cgcagtgcaa ttagggctgg tggcaccgaa ctcgggattt
     8221 ggcacgatca ctgcaaccag cacaaatccc agactcctgc agttttccct gcggctggtc
     8281 ttctagggat ggcgggtgca gagcaggtcg ttttgaaacc tacgctccat agcggcacgc
     8341 cggaaccgcg ccatgcttcc gacgcccggg ataaaccgcc aattgctgct cctaaccggc
     8401 atgcctcaca aaccatgggc ctagaccgaa aggaagtcac aattcgcgag ttgagtgctc
     8461 acaacgagat gcgagcctgt atcgatctgc agaagcgcgt ctggcgtggt tctgacctcg
     8521 atgtcgtgcc tcaaaacatc tttgtggttg cctgtcgaac cggtggactg ttgttggggg
     8581 catttgagcg agacagcctg gtggggttcg cgctcgcgtt tcccgcggta cggcacggac
     8641 acatctatct tcactcgcac atgacagcag tcgcgcccga gcttcaggga cacggcatcg
     8701 gcaagagatt gaagctggcg caacgcgaac atagcctggc ccgcaacttc gatctgatcg
     8761 agtggacctt cgaccctctg cagccgggaa acgccaactt caacattgcc cacctgggtg
     8821 ctattgtgcg cgagtacctg ccaaatttct atggcaacac cacaagcgca ctcgatgccg
     8881 ggctacccac ggatcggttg gtggcggagt ggtggattcg ggaacctcgc gtagagcaga
     8941 tcctggaagg ccgcccccaa ccggcgacgc acgtgcgccg ggcgcgagtg cctcgccgca
     9001 tccgcgaaat ctgcgccgcc aacgcggatg cagcgcgccg cattcagcaa gaacttcgcc
     9061 aggagctgga ggaattgttc cgtacttcat tcgtcattac cggattcgag acagacgccg
     9121 actacgccac ataccttctg gagtgctatg agaatcgaga cgattacgct gcgagagctt
     9181 cgcatgcgtc tcaaatcgcc ctttgagacc agcttcggtg tgacccagga ccgcagaatc
     9241 ctgttggtcg aggcggtggc ggacggcgtc agcgggtggg gagagatcac cagcctggaa
     9301 agccccttgt ttaactcgga gacaacggat accgcctggc acattgtgag cgactatctg
     9361 gccccggcgt tggtcggcaa ggaggtcgct gccgcatcgg aactgcccgc cctgcttgct
     9421 ccctttcgcg gccacgagat ggcgaaggcc ggagtggaga atgcgctctg ggatgcggaa
     9481 gcacagcaga aacgcgtgcc gctgcacgcg ctcctgggtg gaacccgccc ggaaatcgct
     9541 tgcggtgtct cgctgggcat ccgcgaaacg cctgaacagc tggtgagcaa ggtcgaacag
     9601 gaactccggg atggctacca gcgcatcaag ctgaaaatca agccgacgaa ggatctggag
     9661 ttcgtcgctg cggttcgcaa ggaatttcct gaaacgctgc tctcagtcga tgccaactcg
     9721 gcctacattc tcagcgatgc tgctcaccta caaaagctgg atgcattcaa tctgctcatg
     9781 attgagcagc cgttgcagtg ggacgaaatt cacgagcatg cccgattgca ggcgctgctc
     9841 cgtactccga tctgtctgga cgaatgcatt acgaacgcgc gtcatgcgca gagcgcaatc
     9901 gaactcggtg cctgccggat catcaatatc aaactgggac gtgtaggagg tcacaccagc
     9961 gcccgctgca tccaggatat ctgcctgcaa cgcggcattc cggcatggtc gggcgggatg
    10021 atggaatcag gaatcggccg cgctcacaat atcgccatgt cgacacaaca gggcttcacg
    10081 cttccgggtg acgtttccgc cagccggagg tactgggccg aagacattat cgagcctgag
    10141 gtcgaagtga gtccgaacgg gacgatccgc gctccgcaaa gtccaggctt gggttttgcc
    10201 gtaaaacgcg gcctcgtcga acgattaacc tcccgatccc ggagttggtc agccaagaaa
    10261 gacctggtca gcgtgggaga ttgaatgctc aagaggaatc tccattccgt aaggtctgcc
    10321 gcgcagtggc ggctcggtgt ttggattgca accgcaagcc tctcggcgag cgtcatctgg
    10381 gcacctacgc tcctcgcaca aacggcatcg gtagcaggcc ctgggtacga cgtcattatt
    10441 cgaaacggac gaatcatcga tggcacggga aatccctggg tgtcgggcga tgtcgccatc
    10501 cagggtgaac gtatcgccgc cattggcagt ctggccgccg ccaccgccag acgcacgatt
    10561 gacgcgtccg gtctgatcgt ctcccctgga ttcatcgaca tgctggggca gtcggaaatg
    10621 gccctgctga tcgacaaccg ctcgctcagt aagctgtcgc agggcatcac caccgagatc
    10681 acaggtgaag gagactcgat tgctccgcaa aatgcgacga ctctggccag tcttgctcct
    10741 gagctcgacc gctaccaact gaaggtggac tggacgaccc tcgacggata ctttgtccgg
    10801 ctggagaaat caggcacacc gttgaacttt ggcacctatg tgggcgcagc ccaggtgcgc
    10861 gaagccgttc tgggcagcgc gaatcgggcg ccctctcccg aagaactcca gaagatgaag
    10921 gacctggtgg cgcaggctat gcaacagggc gcctttggca tctcgaccgc gcttatttat
    10981 ccgccgggcc actacgccaa gactgatgag ctggttgagc tagccaaggt cgttggccaa
    11041 tacggaggaa tctacgctac ccacatgcgc agcgaaggaa agtttgaggt tcgcgccatt
    11101 gacgaagcgc tgcagatcgg ccgcgaagca cacgtgccgg ttgaaatctt tcacctcaag
    11161 gtgattgggg aaagtcgctg gggatccatg ccgaagatcg tcgccaagat ccaggcggcg
    11221 cgggacagcg gccaggacgt ttcggctgac atgtatccct atgttgccgg cggcacggcg
    11281 ctggccgcca gtcttcctcc ctgggttgcc gatggcggac gaacccaact cctggagagg
    11341 ctgcgcgatc cggccacgcg gtcccgtata aaggaagagc tgaagaccga acatgacgac
    11401 tgggagaatc tatttctcga cagtggtggc ggtcgcgggg ttctaatcgc cggcatagag
    11461 aaaccggcac tcgagcgcta tgacggcaaa acgatcgccg agattgccga gatggagaag
    11521 aagtctccgg tggacactct gctggatctc gtggtcgaag acgaggcccg caccggcgca
    11581 atctatttca tcgccagcga ggacgatctg cagtacggat tgaaacagcc ctggaccagc
    11641 atcgggctgg acgccaacga atcgtcgccg gacggccccc ttttcgaagt gcacgatcat
    11701 ccgcgagcct acggctcgat gccccgattc ctaggacatt atgttcgcga tcagcatctg
    11761 ctgccgcttg aacaagccat tagaaaaatc acatcgttgc ccgcgcaacg cgagcgcctg
    11821 cgcgatcggg gcttgctgaa agtggggtat ttcgcagaca tcacgctgtt tgaccctgca
    11881 accattgtgg accacgccac ctacgaaaac cctgtccagc tttcggaagg tgttaagtac
    11941 gtttttgtta atggtcagct cgagtttgag aacgggcggc cgaccggagc tgccgctggg
    12001 caggtcttgc gcggaccggc gtgggggcag gagcgcaagt gacatggcag ccctggagta
    12061 ctgtcttacc gcgcgttttg gacgcggacc ggagctgcgc cgtaagtttc gcagaatctg
    12121 tcgcagtgca aaagtttcag cctgccagtt gaggccgagg aggaatgccg tgaaaggtcg
    12181 ctctaacagc ttgtggcgat ggtttcgttt ccactcttgg acggttgttg ctgctctaac
    12241 cctgacggca tcgatgtcgg gccagaaacc tcaaacaaat ccttcggccc cgcagaccaa
    12301 accggcgcag ccggccgcgc cgaccgcaaa cggagcggcc tccatgaacg ctgatgatct
    12361 cggcgctttt ctggatggaa tcgtgccaca agaattggca cgggagaaca tcgcaggcgc
    12421 cgtggtcgct gttgtgaaag acgggaaggt ccttttcgcg aagggatacg gatactccga
    12481 cgttaagaag aagacgccgg tttccgcgac tgacactctg ttccgcccgg gatcgatttc
    12541 caagctcttt acctggaccg ccgtcatgca actggttgag caggggaaac tcgacctgga
    12601 tcgggatgtg aacgattatc ttgacttcaa aattccgccg acttattcca agccgattac
    12661 actgcgcaac atcatgacgc acactgccgg attcgaagag accgcgcaag agctcttcgt
    12721 gggcagcgcc aaggacctga aacctctcgg aacatatctg agagaacacc tgccggaacg
    12781 tattttccct ccgggcacca cgcccgccta ctcgaattac gcgactacct tggcgggcta
    12841 catcgtgcag cgggtttccg gacaggacta cttcgactac attgatgagc acattctcaa
    12901 actcttgggg atgacacgca cgacgttccg gcagcccctt cccgatcctc tggccccgat
    12961 gatgtccaaa ggttacagcc tggccaccga tcccgcgaag gatttcgagt atgtggaggc
    13021 ggcgcctgcc gggagttcct caaccacggc gacggacatg acccacttca tgctcgctca
    13081 tctccaggag gggaaatatg agggtgcgca aatcctgcgc ccggaaaccg tgcagatgat
    13141 gcactcgcgc cagttcgaga atctacccag catgaacgca atgtgcctgg gcttttacga
    13201 ggagacgcgg aatgggcatc gcatcatcgg ccatggcgga gacacggaat actttcacag
    13261 cgatcttcat ttgatcccgg acgagaatct cggcttcttt gtgtcataca acagcgccgg
    13321 gaagggggac atacgtccgc gcgaagcgct ctggcacgcg tttctggatc gttacttccc
    13381 ggttcagctt ccggagatcc cacatctcgc cacagctgcg caggatgccg acgccgtctc
    13441 cggacactat ctttccagcc ggcgcccgga gaccaccatc ctccgcatcc tcgcggtcac
    13501 aggagaggca aaggtctatc gcaacgatga cggcacgatc agcgtggacg aactgaagga
    13561 catgagcgga gtccccatta agttcggcga gaccggcccg atgttgtttc gcaaagtcaa
    13621 cggtcaggag caactggggt tcaaacgcga ttattcggga aacacggtcg gggccatcga
    13681 ctttcctttc atgcttcttc agaaagcgtc gtggtgggac aacagcgctc tgcaactggt
    13741 gttgattgtt ggctccttga gcgtcctcgt tttgacgatc ttattgtggc cgatcctggc
    13801 tgcggttcga tggcactacg ccaagccgct caaccttacg cccgaacaac ggcgactacg
    13861 actgctggtg cggctcgccg gtgtcgcgct tgtcgtcttc gttgccgcgt tcgccgcgtt
    13921 tttcacagcc ggggagaaac atatcgaaat actgagtccc aaggggaatc cctggttgcg
    13981 gctcattcaa ttggtgggct ggctgggagt tctgggcagc ctgatcgcaa tctacaacgc
    14041 agtaaagtcg tggcaggacc gcgtccggtg gctctggagc cgcgtcggag acacgctgat
    14101 agcattggcg tgcattggaa cggtgtgctt cgtcttcaca tgtcacatgc tgcactggag
    14161 cttacgatac taggacaggg cgtttattgg gcgttgatct cgacctgcgc cggtcagcaa
    14221 acccgatggc tggaacgact gagagaacca aacctcccag tctctagaca ttagcttcta
    14281 ctgctgacgc gggggcgcag acgccagtgg aacgaagagc aggtcctggt agtcaaaact
    14341 gaagtcggcc aggggcgaga ccgctgacat cgtgaagtgg tcaatcgttc cgtcgggcct
    14401 gagcgcgaac gtgagaaatg cgtcttcgat cttgcggtcc cgccagtgcg ccttgaaggt
    14461 gtcatattgc cagtactcca ggtctccgat cgcgttgggc gtgtggtcga gggtaaagac
    14521 gagtttgccg cccaccagct ttatagtgga cgttccgtac cagtcatcgc tgtaaacacc
    14581 ggcatatttg tcgagaggca atggcggtgc caggcctgcg gcccgtgatg ctgcctgttt
    14641 cgccatcacc tcggctgcat ccttttcccg cgcaagttcc gctttgcgat agggcgtaat
    14701 ccaatcagtg ggcggcaggc tcaggtagta gtcgagaatg tgatacagga tggaattgtt
    14761 cgccgatccc gattcggtat tggtcaggat cacaattccc aggcgcagat caggcaccag
    14821 ttgcacccgc gaaacaaagc ccgcaactcc gccgctgtgt ccgaccagtt tgtatccgtg
    14881 atagtcgttc agaaaccatc ccagcccgta atccgcgaaa ttcggcttga gagccgccaa
    14941 agccggagga acatcggcaa tgggcagaat ggtttgcgga gaccacattt ctttcgattg
    15001 ctgctcacca aataagcgcc gatccgaacc cggcaacctg ccgtggttca gttgcaggag
    15061 caaccatcgc gccatttccg cagcgctcga gttgatggcg cctgccgggg cggcattgtc
    15121 gagggggaca aaatcgatga gctgcatcgt tccgggcagt ttggagtggg gccagccgta
    15181 attgtcgccc ggcttccagg ctgaattggt caaattcgac gaggacatgc ccagaggcgc
    15241 gaaaattcgc tcccggatat tgtcttccca cgacttgccg gttacagccg caacaatctg
    15301 tccggccgca atgtacatca ggttgtcgta cgcaaagcgg ctgcggaagc tgctcttggg
    15361 tttcatgaag cgcagccggt agatgatttc atcccgcgaa tacgtgctgt gcggccaaaa
    15421 cagcaagtcg ccctcgccga gccccattcc gcttttgtgc gtgagcagat cgcggatcgt
    15481 cagctcatgc gaaacgtagg ggtcgtacat ctgaaagccg gggagccgct cgtaaaccct
    15541 gtcgtcccag gagagttttc cttcatctac cagcgaagcc agcagcgccg aggtgaaggc
    15601 cttggtattg gagccgatcc cgaacagcgt gttttcatcg accggaacgg attcacccat
    15661 cttgcgcacg ccgtaaccct tcgccagcac cacgcgcccg tccttcacaa tggcgatgga
    15721 aaggccgggg acttcgaacg tctgcatggc ccgggagatg taaccgtcga ggtctggggg
    15781 tggacccgct tgggcaaata ggggcacgac cgccacccac gcgagcagca gttccgacaa
    15841 gcgtttagcc atgagcctca tatgacgacc gcctccggcg aacacgagtt aggatcggtt
    15901 cggatgatgc aaaagccgcg aagaggagcg caaccctgat acatcatgtg atgtagtaat
    15961 gtcaatcgca tcatcagttg ttgcagatga accctaggcg tgataaacaa agactccgtt
    16021 ccttcgagag ggcacctggg agtggaagag atagccggga agactgcagc cgtgccattg
    16081 gattccggca aaagcctgct cgcgcgcgtg ctacgctttc gcgatctggt ggtcctggtc
    16141 atcggcacgg tcatcggatc gggaatcttt ctcgttcccg gggcaattct gcgtcaggtg
    16201 ggcaattccg ttccgctggc caccgctgtg tggctgatcg gaggtctgct gtctctgctg
    16261 ggcgcgctca cctgtggcga actcagcgcc atgaagccac aagcgggcgg ggtctatgtc
    16321 tatattcgcg attgttttgg gccgttcgtg gctttcctgt tcggctggac gctgttcttt
    16381 gtgatcagct caggatcgat cgcgaccttg gcggttgcct tctgcgacta ttctgctgaa
    16441 cttgtcccgc tgaatccctt gatgggtcgg ctggtgtcgg ttgtgatgat cactgtgatc
    16501 gcgctggtga atgtgctcgg cacacgccga agcgcagacc tgcaagacgt aaccactgcc
    16561 gtcaaggtag ccgccattgt gctgatggcg tccgccttgc tggggctcgg caaaggctcc
    16621 tcctctgttc ccgttgcgcc tcacgacctg agcgctggcg gatacgcgtc gggctttggc
    16681 ttggcaatga tcagtgtgct gtgggcctac gagggatggc agtacgcgac cttcagcgcc
    16741 ggagaagcca tcgatccgca gcggaatttt ccactcgcgt tttttgccgg ttcggcggca
    16801 ttgattgcca tctatcttct ggcaaatttc ggctatctgg cggcgctcgg cccagccggc
    16861 gtgtccgcct ccgatcgtgt ggcagccgcg gcggtttccc gcgtcatcgg tccagccgcg
    16921 gcgaagctgg tggcgctcac cattctggtc tcgatcttta gcgccactaa cggcatcgtg
    16981 ctcaccgcct cacgggtcta ttacgcaatg gcccaggacg gtctgttctt caagcggctg
    17041 gcggaggtgc atcagcggtt caagaccccc gcctttgcag tcgtcgcgtg ttgcgcttgg
    17101 tcagctgttc tggcagtcac cggcaccttt gagcagcttc ttacttatgt cgtgttcatt
    17161 ggctggattt tctatgcgct cgccgcgggc acggtattcg tatatcggaa acgggaaccg
    17221 gaagccgatc gcccctacag agtcccaggg tatcctttca ctccagcctt gttcattgct
    17281 gccgctgcgt acctggtgat caacaccgtg gtgacacagc cgcggcgcgc cgctgcaggg
    17341 atagccattg tcctgctcgg cgctcctgcc tacgcgatct ggcgccgcaa agagcgtact
    17401 tcatgacgcc ggcttggcgg atcgtggcag gggatggtcc agccttcact ggagaacgcg
    17461 tcgcgcccgc catcgcagaa agcgttgtat cgatggcgaa tctgccgggt gcgatttgat
    17521 gtcggggctg cctcaagcat gtcctcaacg gctttccgcg accgctgatt gatccgtttg
    17581 agcaatgcag gcccacctac gtccgcagag actcggtaga gagcctttcc cggttcactc
    17641 ttaatacaag ctcccgcagg atgacctcgc agtattcctg gcgttcttcc ccatccagtc
    17701 cgcccaaaag gacctggaat cgctgtgacg gcttcagcca gtcttctatc gagcggctgg
    17761 tcatgatgac tttgccgcac gcgccgcgaa tggcgtcgag gaggcggcgc aggtcggcac
    17821 ggtctgactg tcggtcaggc caccagagca cgttgtttct ttcaaatgtc gatgcagtgg
    17881 tgtgccaagt gtaggcgggt ttggcgggat caaaaattga gcatcaagcg ttcaaaaaag
    17941 gcctctatgt gactcactcc ttggacatgg atcgcacgcc ctgttgcatt agccacctgg
    18001 caattgcagc agagtcgaat ctcacacagg aacccactcg aaacgacgga atgctaccgc
    18061 gcttcgcccg ccggagaatc gttatgcggg acatctgaag taactcagca agctcttcag
    18121 cggtcagagc acgatgaatg gcatggatgc gcgaggcgag cgacatcagc ctcatttccc
    18181 tttccgagct ctcagtcctc tacttaatag atagtcaaaa gtcggaaaac ttaggcgctg
    18241 attgcgcggc tccgcaggct cccgcgccct ttctcggatt acggcactgg aagcctgacc
    18301 gatccgcccc acagacgcac tccgggatga cctccagggc cggttgccct tacagcccga
    18361 tatggactcc atttacgatc tcccgaacct catccgtcgt gtcatcgccc aaggcaaggt
    18421 aacgaagtgt cgtttccaga tcggcgtgcc ctagccactt ctggattttc cgggccgagg
    18481 ctccggcgtt gtggcggtcg gtcgcaaagt tcttgcggaa gcggtgcaga atccacttct
    18541 cacagaccgg gccatcgcca caggagacta cctttccgtc cgaagttccc ttgcacttgc
    18601 cgcagttcag accgccgtcg aacgcgactc gcttgagccg acgtagaatg tgaccatccg
    18661 ggcgaccgtt gttatcgaag agcagtgagc aggagccgga tttcgcacgc gcatctatct
    18721 cggcgatcag agcatccggg atgggcaccc aacgcttttc gcagtccttc ggcttgaatc
    18781 cgaactctgg cttctggtcc acgcggatca tcttcgtatc atgattcacg tctgtgcttt
    18841 cagcgaccgc cgtctcggat tcccggaagc cagtcccaag gaagaaccgc cacaggaagc
    18901 gttcatcctc gtccgacact tggaacaggt cttcaagctc ccgggtgtac gcctcaggcg
    18961 gtgactcgac gtacttgatc gtaatggtca cgtccgtaat tggtgggccg gggcgacgtc
    19021 cctgctcgtt gcgaagaaag gtgacaatgt ggccgactcg gttggaccgg gtgcggtcat
    19081 cattgcctcg ctggacaagg aacgtatcga gcgcctgcaa atcctgcttg ccgatttgat
    19141 gcatgtaggt cttcttgcag gattcttgaa agagttcgag gctcttcgcg tatgccttgt
    19201 aagtcttggc tgccaatttt gcagcattga tgaggtactc gcgcacttcg tcagtgacgc
    19261 ggtatccagg cgctttttcg gctgccgctg gctgttccgg ctggctggtt ttcaggccca
    19321 gaacatcttc ggttccgtgg gcctcctgct tcgcttgggc tttcgctttg cacgacgccg
    19381 tgtgcggatc agttccgagc gactcccact tctcttttcc atcgcgcttc acacgctggt
    19441 agtagtagcc gtttgggtgg aattctggct ggccgtttac caggcaccag aaatcgcgca
    19501 ggcgggactt gctgtcgcag acggcggggc agtagggaga ttgctttccg tctggggttc
    19561 ggaatctgag gtatagcttg atattgggct tacggggcat cctgattttc tttcctatct
    19621 ggtaaatacc cagatcaggc gggaaaatca gtgcaagttc gtagctatct cataaaccct
    19681 ttgccatgaa ggactggcgg agagggaggg attcgattac cgtcgtttct agcaagtcgc
    19741 ggtgattcct acacatagcg ataataccct gtgtttataa gggtttcaag catattctcg
    19801 acttagggtc ggttgtctga gacgacctct aacgacctgc attgcccata aaaacggtat
    19861 cactggtatc acctgcaaat ggcgtcgtcc ccttggccgc tgagaacac
//
LOCUS       DASPUL010000539         3640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_780192, whole genome shotgun sequence.
ACCESSION   DASPUL010000539 DASPUL010000000
VERSION     DASPUL010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780192"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VEJ00_12605"
     CDS             <1..407
                     /locus_tag="VEJ00_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02048.1"
                     /translation="WHGQTAWLMHFQQRDDKPSHFADYVTGGRRYPMKLKGRAWIASD
                     NLQIVRIESDLVSPLPQFTVSHQIAEYGPVHFNKVNADLWLPQHVDIFMEMNRHYYHR
                     QHSFDHFMLFAVDSEDKGPLRKPQGSAQTQNP"
     gene            545..1567
                     /locus_tag="VEJ00_12610"
     CDS             545..1567
                     /locus_tag="VEJ00_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXX02049.1"
                     /translation="MRNSRNGSGTRHSKTIVFLAAPNSQILDVAGPFQVFVRAAELFV
                     QEHPGRERPYHVALASTSPRKAVTTNCGLVLTGSETFRTLRGPIDTLLVAGGSGLDTA
                     AQDKKLLAWLRKTAPRVRRFGSICTGAFLLAAAGLLDGKHVTTHWKWAADLAKRCKRA
                     TVDPDPIYIRDGNTYTTAGVTAGMDLALALVEEDLGSPLALRVARELVLYLRRAGGQS
                     QFSTAMSLQASDRKQIEEIRAWALDHLDQQLPVDKLAAKAGMSPRNFARVFVKDTGST
                     PARFVERLRVEAARRRLEESRDKLEKIANDCGFGSVTSLRRSFLRVLRVPPADYRHRF
                     SEANSN"
     gene            1630..2565
                     /locus_tag="VEJ00_12615"
     CDS             1630..2565
                     /locus_tag="VEJ00_12615"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HXX02050.1"
                     /translation="MKRTVWLLVVALSAGMLILSLSGMNSSTALRAAARPPENKQPDV
                     ANALTPPAKGDIPVAFLLSEGAVMIDFTGPWEVFQDVHLPNGSMPFRLYTVGPTLHPI
                     HASGGMKIIPDYSIANAPVPKILVIPAQSELGPATKAWVRKVAKSADVTMSVCTGAFA
                     LASTGLLSGKPATTHHGAYNSLATKYPDIQVKRGARFVEAGNLASAGGLSSGIDLALR
                     VVERYYGHDAARQTAFDMEYQGEGWMNRDSNSVYAHPYAQVDEHPVCPVCGMEVDPAT
                     APKSVYNGKTYYFMNEQHKQQFDADPQSYVGINKH"
     gene            complement(2656..3159)
                     /locus_tag="VEJ00_12620"
     CDS             complement(2656..3159)
                     /locus_tag="VEJ00_12620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX02051.1"
                     /translation="MIRRCTDVDFQTIEIIVNEAAQAYHGVIPADCWHEPYMSGAALK
                     AEIDAGVNFWGWDESDTLLGVMGIQDVRDVTLIRHAYVRTAQQHRGIGGNLLGFLADQ
                     ARGPLLVGTWTAAVWAIRFYERHGFRLVSQPEKDKLLNAYWSISSRQRETSVVLVSTR
                     HPQPPQP"
     gene            complement(3156..>3640)
                     /locus_tag="VEJ00_12625"
     CDS             complement(3156..>3640)
                     /locus_tag="VEJ00_12625"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal ligand-binding domain-containing protein"
                     /protein_id="HXX02052.1"
                     /translation="RVAAESGVAKTTLYRHWPTKAALCMDLYLDISGRELNLPDTGNI
                     ASDLRHIFSTVVHLQTRTVAGPAFLGLIAEAQVNPATRKAFLAEFADRRREVTRAVLK
                     RAIQRGEIRADTDVDLFIDALGGAVTFRLLQGHAPLTTSFANALIKLLLAGCSTKESR
                     "
BASE COUNT          757 a         1109 c         1077 g          697 t
ORIGIN      
        1 attggcatgg gcaaaccgcc tggctcatgc atttccagca gcgcgacgac aaaccgagcc
       61 actttgccga ctatgtgacc gggggccgaa ggtatcccat gaagctgaaa ggccgcgcct
      121 ggatcgccag tgataacttg cagatcgtgc gcatcgagtc cgatctggtt agccccttac
      181 cccaattcac ggtcagtcat cagatcgccg aatatggccc cgttcatttc aataaggtga
      241 atgccgatct gtggctgcct cagcacgtag acatcttcat ggaaatgaat cgccactatt
      301 atcaccggca gcacagcttt gatcacttca tgttattcgc ggtcgattct gaagataagg
      361 ggccgctgag aaaaccgcag ggcagcgccc agactcaaaa tccctgagct gccttaacct
      421 tccaagcttc aacccggaca ccaacaacgc ccggaatcaa ggcaagaagc tgttttctgc
      481 caatggcaga aagtgacgtg tttttgtcat tgctgccatt cttcctgacg tctaccatcg
      541 cggcatgagg aactccagga acggctccgg aactcgccac tctaagacga tcgtttttct
      601 ggctgctccc aatagtcaga tactcgacgt ggcaggcccg ttccaggttt ttgtccgcgc
      661 cgcggagttg ttcgtgcagg agcatccagg acgggagcgg ccgtaccacg tggcgctggc
      721 cagcacgtcc ccgcgcaaag cggtcacgac gaactgcggc ctggtcctga ccggaagcga
      781 gactttccgc acgcttcggg ggccgatcga cacgttgctc gtcgccggcg gcagcggcct
      841 cgatacggcg gctcaagaca agaaactact ggcttggctg cgcaagacgg ccccgcgcgt
      901 tcgccgcttc ggatcgattt gcaccggagc cttcctgctc gctgccgcag gacttctcga
      961 tggcaagcac gtcacgactc actggaagtg ggcggcggac ctggccaaac gctgtaaacg
     1021 cgcgactgtc gatcccgatc cgatctatat ccgcgatgga aacacctaca ccacggcggg
     1081 agtcacggcg gggatggatc tcgccttggc attggttgaa gaagacctcg gttcgccgct
     1141 cgcgttgaga gtcgcgcgcg aactggtgct ctacctgcgc agagccggag gccagtcgca
     1201 gttcagtacg gcgatgtctc tgcaagcctc ggaccgcaag cagatcgagg agattcgagc
     1261 ctgggcgctc gatcaccttg accagcaact gccggtcgac aagcttgcgg ccaaggctgg
     1321 aatgagcccg cgcaacttcg cccgcgtctt cgtgaaggat acggggtcta ccccggcacg
     1381 attcgtcgag cgtctaagag tcgaggcagc gcggcgtcgg ctcgaggaat cgcgcgacaa
     1441 gctcgagaag attgcaaatg actgcggctt cgggtccgtg accagcctgc ggcgttcatt
     1501 cttgcgcgtg ctgcgcgtgc ccccggcgga ctaccggcac cgattttcgg aagcgaactc
     1561 gaactagaaa gcggagtcgt agtgcttact cagaaatgaa cggcgcagat ctgtcatgga
     1621 gatgaacaca tgaaaagaac ggtgtggttg ctggttgtag cactcagcgc agggatgctc
     1681 atcctttcac tcagcggaat gaattcgagt accgctctca gagcggccgc gcgcccgccg
     1741 gaaaacaaac aaccggatgt tgcgaatgct ctgacccctc ccgccaaggg cgatattcca
     1801 gtggctttct tgctctccga aggcgcagtc atgatcgact tcaccggtcc gtgggaggtc
     1861 ttccaggatg ttcatctgcc caacgggagc atgccgttcc ggctttatac ggtggggccg
     1921 actctgcacc ccatccacgc cagtggtggc atgaagatca tccccgacta cagcattgcc
     1981 aacgctcccg taccgaaaat tcttgtgatc cccgcgcaga gcgaactggg gccagcaacg
     2041 aaagcgtggg tccgcaaggt cgccaagagt gccgacgtca ccatgtcggt ctgcaccggc
     2101 gcatttgcgc tggcaagcac gggtctgctg tcagggaagc ctgcgacgac gcatcatggc
     2161 gcctacaaca gcctggccac gaagtacccc gacattcagg ttaagcgcgg agcgcgcttc
     2221 gtggaagccg gcaatctggc cagcgccggc gggctgtctt ccggcatcga tcttgcgctc
     2281 cgggtcgtcg agcgctacta cgggcacgac gccgcacgcc agaccgcgtt cgacatggaa
     2341 taccagggag aaggctggat gaaccgtgac tcgaactcgg tctacgccca cccctatgcc
     2401 caagtcgatg aacatccagt ttgtcctgtg tgcggcatgg aggtcgatcc ggctacggct
     2461 cccaagtccg tctacaacgg caagacctac tacttcatga acgagcaaca caagcagcaa
     2521 ttcgatgccg atccgcagag ttacgtcggg ataaacaagc actagcagat gcagccgagt
     2581 ctgcgagcag aacggctccg agcaagccag ccggatcgct ctggccttag cgtgcttgca
     2641 agggaggggc ggtgactacg gctgaggcgg ttgaggatgg cgtgtagaga cgagtaccac
     2701 agacgtttcc ctctgccgtg aagaaatgct ccagtaggcg ttcagaagtt tgtccttctc
     2761 cggctgcgag acaaggcgaa agccgtgtcg ctcatagaac cggatggccc acacagccgc
     2821 ggtccacgtg ccgaccagca aaggtcccct ggcctggtcg gcgaggaacc cgagcaggtt
     2881 cccgccgatt ccccgatgtt gctgtgccgt acgcacgtag gcatgccgga tcagcgtgac
     2941 gtcgcgaacg tcctgaattc ccatgacccc gaggagagtg tcggactcgt cccagcccca
     3001 gaagttgacg ccggcgtcga tctcggcctt gagtgcagcg cctgacatgt agggttcgtg
     3061 ccaacaatct gccggaatca cgccgtggta ggcctgggcg gcctcgttga ctatgatctc
     3121 gattgtctga aagtcgacat cagtacagcg ccggatcatc gcgactcctt cgtgctgcaa
     3181 cccgcgagca gcaacttgat caaagcattc gcaaagctgg tggtaagcgg ggcgtggccc
     3241 tgcagtagcc gaaaagtcac cgcgccaccc agcgcatcga tgaagaggtc aacgtccgtg
     3301 tctgcgcgaa tctctccacg ctgaattgcg cgcttcaata ccgcacgagt tacttccctg
     3361 cggcggtcgg cgaactctgc caggaaagcc ttgcgtgtgg ccgggttcac ctgcgcttcc
     3421 gcaatcagcc cgaggaaggc ggggccggcc acggttcgtg tctgcagatg cacgacggtg
     3481 ctgaagatat gccgaagatc ggacgcgata tttccggtgt ccggcaaatt gagctctcgt
     3541 ccagagatgt ccaggtagag atccatgcac agcgctgctt tcgtcggcca atggcggtac
     3601 agcgtggtct ttgccacccc gctttccgct gccacgcgct
//
LOCUS       DASPUL010000540         5691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_780256, whole genome shotgun sequence.
ACCESSION   DASPUL010000540 DASPUL010000000
VERSION     DASPUL010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780256"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..834)
                     /locus_tag="VEJ00_12630"
     CDS             complement(13..834)
                     /locus_tag="VEJ00_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02053.1"
                     /translation="MPIPHAQLATTQISVINESTVLSDGDVIPVVTALQQQVTNDFRP
                     IWGTDAELKMIPQGTQPPAGTWWLVILDDSDQAGALGYHDLTTDGLPLGKVFAASDLK
                     AGTSWTVTASHELLEMLADPNINLTVFVQNANTSGTLYAYEVCDACEDDSLGYQINNI
                     LVSDFVYPAWFENFRTQGSTQFDRMNKMQNPLQLLPNGYIGVFNVSDGTGWQQQTAEK
                     IPTNSRHRGHVGSRRERRSISRDQWVMSLPQRKITNNAQQYRQHLQTLQRRKPAA"
     gene            complement(1139..2374)
                     /locus_tag="VEJ00_12635"
     CDS             complement(1139..2374)
                     /locus_tag="VEJ00_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXX02054.1"
                     /translation="MKQRKVVTSPLVIALALVIARSPWAAGQTTSTAAAPAPNFAEQQ
                     KPIFKLMHVEEGWEITRGEPNCVIGVIDTGFDFFHPALAGHIKPGWFAPGVYHTDFFT
                     MDAHGTLVSSLLVAHRQEGQDGMWGFAPGCSVLAAAQGMPVHELLRWRQNFLAKNPNA
                     SGAEMAKAMSQTPSLKAFGDHWIEFVFTTLADSIHYLTDHGARVINISEFIATSVLAS
                     KPELKARVEEAFNYARDKDVLIVVGAGNSDNPIAEYPGDRDSVMVAGASTLADKRWSS
                     KVSYMGMDVPQGSCYGPRLSVMAPVQGLVVASPHEDAYYSWNDTPIGSQKEKFEAMYE
                     VLPWGATSSATPEVAALAALVRSLRPDLHTKDVIRLIEQGADPIGDAGFHEETGYGRI
                     NFFKTLELARAVPDLPIHK"
     gene            complement(2494..2739)
                     /locus_tag="VEJ00_12640"
     CDS             complement(2494..2739)
                     /locus_tag="VEJ00_12640"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXX02055.1"
                     /translation="MPEGRPTIRNHIRRLRFESGELSQEELGKRCGVTRHTIMALEAG
                     KYSPSLLLAFRIARAFGTGVEQVFEYGDIGNPGRPVS"
     gene            complement(2732..3142)
                     /locus_tag="VEJ00_12645"
     CDS             complement(2732..3142)
                     /locus_tag="VEJ00_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02056.1"
                     /translation="MTDSEKWSVWTLGVIALTTTAYFIFVALRGHGPATISVFALTAL
                     VAINKRSFRSVIGPRFDERESEIARKALLTSFRVMWLAVVAGFVITGHIKGWDTTLSL
                     PMWKLAEIFWWLSMLMLAVQAGTTLLLYRRGNNA"
     gene            3276..4376
                     /gene="queG"
                     /locus_tag="VEJ00_12650"
     CDS             3276..4376
                     /gene="queG"
                     /locus_tag="VEJ00_12650"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HXX02057.1"
                     /translation="MNSVQMSEFTAVVKRAASEAGFDLCGVAPATDAPELQYFPAWIG
                     AGHAGEMKYMEARDEQGELKRASLHRVAPWARSVIVCAINYNTDHPYSTQVQEPGRGW
                     ISRYAWSREDYHDAVMRRLKEVEMAVWSGHSLRQAQGKLCPPSLTTRCYVDTGPIVER
                     VFAKYAGVGWIGKNTCVINQKKGSWLFLGVILTSLELAPDLPAPDRCGTCTRCIEACP
                     TDAILAPYRLDSNRCISYLTIEKRGTIAEDLRTDMGRHVFGCDICQDVCPWNRKAPAS
                     TAAEFEPRPGLVNPALDWLAEMSEEEFRQNFRGSPIRRTKRSGLRRNAAIAMGNSGDR
                     KFLPLLDKLAGDADEAVSESALWAKNRLLGNT"
     gene            4534..>5691
                     /locus_tag="VEJ00_12655"
     CDS             4534..>5691
                     /locus_tag="VEJ00_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02058.1"
                     /translation="MLSRHIPCMRRAAARLAIVVLACVSLSRASATLLLEEPYGKLGF
                     FTATGHAAVYLSGVCAETPVLLRPCTPGETGVVISRYDGVGGYDWVAIPLIPYLYAVD
                     RPEDIPLFADAKMVAFLRDRYRRRYLEEIAPDVKNGETPGGNWYELVGSSYDRTIYGF
                     EIETTPEQDRALIGKLNSSPNESHFRTASNNCADFAKDIINFYYPRALHRSVIADVGI
                     TTPKQMAKTMVKFNRRHPDISYSRLVIAQVPGSMARSSTVHGVVESFFKSKKYIVPSA
                     VVSPIFAGCVFAVYVGTGAGHFEPARDALVFDASSAPEPPLGREDRLAYQHQLKNFLT
                     GTYPVRPDRMWERHGRGCRPRRSRISTRKAARSCRCRWMAGSCGSEPRPTMS"
BASE COUNT         1150 a         1698 c         1642 g         1201 t
ORIGIN      
        1 ctacccggcg tatcaggctg ccggttttcg tcgttgcaac gtctgcaagt gttgccggta
       61 ctgttgtgcg ttgttggtga tcttgcgctg gggcaagctc atcacccact ggtcgcggga
      121 gatggaacgg cgctcgcgcc gcgagcccac gtggcccctg tggcgactgt tggtcggaat
      181 cttctccgct gtttgctgtt gccagccggt gccatcgctc acgttgaaca cgccaatata
      241 gccgttgggc agcaactgaa gcgggttctg catcttgttc atgcggtcaa actgggtcga
      301 tccctgcgtg cggaagtttt cgaaccacgc cggatagaca aagtctgaaa ccagaatgtt
      361 gttgatctgg tagcccaggc tgtcatcttc gcaggcgtcg cagacctcgt aggcatagag
      421 cgtgccgcta gtgttcgcat tctgcacgaa cacagtcagg ttgatgtttg ggtcggcgag
      481 catctcgagc agctcatggc tcgccgtgac cgtccacgaa gttcccgctt tgagatcgct
      541 ggcggcgaag actttgccga gaggcagacc gtccgtcgta agatcgtgat agccgagcgc
      601 cccggcctgg tcggagtcgt ccagaatgac cagccaccag gtccccgctg gcggctgcgt
      661 gccctgggga atcatcttga gttctgcgtc tgtgccccag atggggcgaa agtcgttggt
      721 gacctgctgc tgcagggcgg tgacgaccgg tataacgtcg ccatcgctca gcacggtgct
      781 ctcgttgatg acggatatct gagttgtcgc gagttgcgca tgaggaatcg gcatccgtat
      841 tccctccttt tacctgacga attttttcgg agaacgcagc ggcgaagatg cgtgactctg
      901 tatcgggaag cttataccct gaaaaaacgg aggcaaggat tatccgagca accgcgcgcg
      961 gattccggat ttgggattcc agtttgggac ggctgaagga gtcagaaggt ggaagtcaga
     1021 atgaagaagt tcaacgccgc cataaaacgc agcgaaattg cccggggttc gggcgatttc
     1081 gcgtagggga ggaacccttc agcaagctca gagcgggccc tgcttatgcg ggtgcatttc
     1141 acttgtggat aggcaaatct ggaacggccc ttgccaactc tagggtcttg aagaagttaa
     1201 tacggccata gccggtttcc tcgtgaaagc ccgcgtcacc aatcgggtcc gcgccctgct
     1261 cgatcaaccg gatcacatct ttagtgtgta ggtcgggacg aaggctgcga accagcgccg
     1321 ccaacgcggc cacctcgggc gtagccgacg aagtcgcgcc ccatggcagt acctcataca
     1381 tggcctcgaa tttttctttc tggctgccaa tgggagtgtc gttccacgag taataagcgt
     1441 cttcgtgtgg gctggcgacc actagtcctt ggaccggtgc cattacgctc aatcgcggtc
     1501 catagcagga tccttgcgga acatccatac ccatgtagga gaccttcgag ctccagcgtt
     1561 tgtcggccaa ggttgaagcg ccggctacca tcaccgagtc gcggtcgccc ggatactcgg
     1621 caatcgggtt gtcgctgttg ccggcgccaa ccacaatcag aacatctttg tcccttgcat
     1681 agttgaaagc ctcttcgacc cgcgctttca attccggttt cgaagccaga accgacgtcg
     1741 ctatgaattc gctgatattg attacacgcg caccatgatc ggtcaaatag tggatcgagt
     1801 cggccagcgt ggtaaaaacg aattcgatcc agtggtcccc gaaggccttg aggctcggtg
     1861 tttgcgacat ggcttttgcc atctccgcgc cagaagcatt cgggttcttt gcgaggaagt
     1921 tctgccgcca gcgcagcagt tcatgcaccg gcatcccctg cgccgcggct aagactgaac
     1981 agccgggcgc aaagccccac attccgtcct ggccctcttg acgatgcgct accagcaagc
     2041 ttgaaaccag cgttccgtgc gcatccatag tgaaaaaatc ggtgtgataa accccaggcg
     2101 caaaccaccc cggcttgata tgacccgcca atgccgggtg gaagaaatcg aaaccagtgt
     2161 cgatcacgcc gatgacgcag ttcggttcgc cacgcgtaat ctcccaaccc tcttcgacgt
     2221 gcatgagctt gaagattggc ttttgttgct ccgcgaaatt gggcgctggg gcggcagcgg
     2281 tcgaggttgt ctggcctgcg gcccacgggg atcgtgcgat gaccaacgca agtgcaatga
     2341 ccaacggaga tgtgacgact tttcgttgct tcataagttt tcctatttct tcgggatgcg
     2401 ttttgggagg cagccccggc acccgacgga gagaggcgcc gcgaggcggc cgcatacaag
     2461 gattctggaa ctgctctgtc gggaacgacg cgatcacgac acgggacgcc ccggatttcc
     2521 aatatcgcca tactcgaata cctgctccac tcctgtgcca aaggcgcgcg caatgcggaa
     2581 tgcgagaagc aatgacggag agtattttcc tgcctccaat gccatgatgg tgtgccgcgt
     2641 gacgccgcat cgctttccca actcttcctg agacagttcc ccgctctcga atcgcagccg
     2701 gcggatgtgg ttgcggatgg tcggtctacc ctcaggcatt gttccctctc cgatacagca
     2761 ggagcgtggt cccggcttgc accgccagca tcagcatgga caaccaccag aagatttccg
     2821 ccagcttcca cattggcagc gagagcgtcg tgtcccagcc tttgatgtgt cccgtgatca
     2881 cgaacccggc cacgactgcc agccacatca cgcgaaagct cgtcaataac gctttgcggg
     2941 cgatctcgct ttcccgttcg tcaaagcgcg gcccaatgac gctacggaaa ctcctcttgt
     3001 tgatggcgac cagtgctgtc agcgcgaaaa cgctaatagt tgccgggcca tgaccccgca
     3061 atgctacgaa gatgaaatat gcggtggtcg tcaacgcgat cacacccagc gtccacacgg
     3121 accatttctc agaatctgtc atatctacat tccataatgt catctataaa taactatatg
     3181 ttatgtatac ataacatata cagccatcta tgtcaagcgc gatttttggg atttttgact
     3241 ttgggaccct aacttctgca tgcttcggcg aacgaatgaa cagcgtgcaa atgtcggaat
     3301 tcactgccgt cgtcaaacgc gcggcatccg aggcaggctt cgatctctgc ggagtagctc
     3361 ctgcgacgga tgcgcccgag ctacagtatt ttccggcctg gatcggcgcc ggccatgccg
     3421 gcgagatgaa gtacatggaa gcgcgcgatg agcagggcga actgaagcgc gcttcgctcc
     3481 accgggttgc gccgtgggcg cgaagcgtga tcgtctgcgc gatcaactac aacacggatc
     3541 atccctattc cacgcaagtg caagagcccg gccgcggatg gatttcgcgc tacgcctgga
     3601 gccgcgagga ctatcacgat gccgtcatgc ggcgcttgaa ggaggtggaa atggccgtgt
     3661 ggagcggaca ctcccttcgg caagctcagg gcaagctttg cccgccaagc cttaccaccc
     3721 gctgctacgt cgacaccggc cccatcgtcg agcgcgtgtt cgcgaagtac gctggagtgg
     3781 gctggatcgg caagaacacc tgcgtcatca atcagaagaa aggctcgtgg ctgttcctgg
     3841 gtgtgatcct tacttcgctc gagctcgcgc ctgacctgcc cgcgcccgat cgctgtggca
     3901 cctgcacgcg ctgcatcgaa gcctgcccga ccgatgccat cctcgccccg tatcgcctcg
     3961 actcgaaccg atgcatctcg tatctcacga tcgagaaacg aggaacgatt gccgaagacc
     4021 tgcgcacaga catggggcgc cacgtcttcg gttgcgatat ctgccaggat gtgtgtccct
     4081 ggaaccgcaa ggcaccggcg tcaaccgcgg cggaattcga accccgcccc ggcctggtca
     4141 atcccgcgct cgattggctg gcggaaatgt cggaagaaga atttcgtcag aactttcgcg
     4201 gatcgcccat ccggcggacc aagcgcagcg gcctccgccg caacgccgcc atcgccatgg
     4261 ggaacagcgg agaccggaaa ttcctccccc tgctcgacaa gctggccggt gacgccgacg
     4321 aagccgtttc ggagagcgcc ttgtgggcga aaaaccgtct tctcggcaat acgtaggacc
     4381 cctgattttg gctcttcctt cattaccaag tgcatctctt ttcaatgctg attcctttga
     4441 acttcggtaa ctggtttttc cgatccccag caactaatct tcggctaacg ggttctaact
     4501 aaggaggtta ggctcaagtc gtctggggtg accatgcttt cacgccacat cccgtgcatg
     4561 cggcgcgctg cggcccggct cgcaatcgtc gtgctggcct gcgtatcgct atcgagggcc
     4621 tcggccaccc tgcttctgga agagccttac ggcaagcttg gcttcttcac ggccaccggt
     4681 cacgcggccg tgtatctgtc cggcgtgtgc gccgaaactc cagttctgct gcgaccttgc
     4741 acaccgggag aaactggcgt cgtgataagc cgctacgacg gagtcggcgg atacgactgg
     4801 gtcgccatac cacttatccc gtacctgtat gcagtcgacc gtcccgaaga cattccgctg
     4861 tttgccgacg cgaagatggt cgccttcctg cgcgatcgtt atcgccgccg atacctcgaa
     4921 gaaattgctc ctgacgtcaa gaacggcgaa actcccggcg ggaactggta cgagctggtg
     4981 gggtcctcgt acgatcgcac gatctacggc tttgagatcg aaaccactcc ggagcaggac
     5041 cgggcgctta ttggcaagct caactcgtcg cccaatgagt cgcacttccg cacggcgtcg
     5101 aacaactgcg cagatttcgc caaagacatc atcaacttct attaccccag ggccttgcat
     5161 cgcagcgtga tcgccgacgt cggcatcacc acgccgaagc agatggcgaa aacgatggtg
     5221 aagttcaacc gccgccatcc cgacatctcc tattcgcggc tggtcatcgc gcaggttcct
     5281 ggaagcatgg cgcgcagctc gaccgtgcac ggagtggtcg agtcgttctt caaatcgaag
     5341 aagtacatcg tgcccagcgc cgtggtcagt cccatttttg cgggctgcgt ctttgcggtt
     5401 tacgttggaa cgggcgcagg tcacttcgaa ccggcgcgcg atgccctggt gtttgacgca
     5461 agcagcgcac ctgagcctcc gctgggccgc gaggaccgcc tggcctatca gcatcagctg
     5521 aagaactttc tgacgggcac ttatccggtt cggccggaca gaatgtggga aagacatggt
     5581 cgaggttgca ggccaaggcg aagccggatt tcgacgagga aggccgcccg gtcctgcaga
     5641 tgcagatgga tggccggctc gtgcgggtcg gagcctcgcc cgacaatgtc c
//
LOCUS       DASPUL010000541         4490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_243180, whole genome shotgun sequence.
ACCESSION   DASPUL010000541 DASPUL010000000
VERSION     DASPUL010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243180"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /gene="dprA"
                     /locus_tag="VEJ00_12660"
     CDS             complement(<1..1088)
                     /gene="dprA"
                     /locus_tag="VEJ00_12660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXX02059.1"
                     /translation="MTTVSASPGATHLLEWLAIALTPGLGPTKARKLVEHFGAPEAVF
                     RTSLTELESTGIQAVSAQSLATGRSAELARDEIARAAAVGVAVISMDDPAYPPRLKEI
                     YDPPLVLYIRGNPEILTKPGIAMVGTRHPTPYGTGMAERLACDLAMQGLVIISGMARG
                     VDTAAHRGAISAKGKTIAVFGTGVDVIYPKENSRLSEQMLALGGALISEFPLGTFAAP
                     QNFPIRNRILSGMSFGVLVVEAAEYSGTRITARCALEQNRDVFAVPGNVTNKNSWGPN
                     TLIKQGAKLTATWEDVWEDLPADVRLALTPASSTESPGSSSASLFPEEGLPPHEKRIL
                     GLLKADEATHIDEIVEKLETELSSSEIFA"
     gene            1188..2021
                     /locus_tag="VEJ00_12665"
     CDS             1188..2021
                     /locus_tag="VEJ00_12665"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HXX02060.1"
                     /translation="MRLRISAISYLNTAPLMWDFEHGDAGRQFDISYTLPSACARALA
                     EGAADIGIIPAAAYTQIPGLQILPDVTIASRRAVRSILLVSKQPIEKVRTVALDTSSL
                     TSVALTKVLFERWLGGARTYTPMAPDIEQMLAECDAGLVIGDPALKIDCSRYLTLDLS
                     EEWIRHTGKPFVFAFWAVRGGALREADSALDLPTIFRESRDNGLTPASLNQTAREWAP
                     RFGLTEAEVHSYLTENIHYQLDSGCLEGLQLFYRYAAEIGALPAAPDLHFVEAAKAIA
                     T"
     gene            complement(2033..>4490)
                     /locus_tag="VEJ00_12670"
     CDS             complement(2033..>4490)
                     /locus_tag="VEJ00_12670"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXX02061.1"
                     /translation="PDRLRRFEQEARAAAALNHPNILAVYQFGTHEGAPYLVSELLEG
                     STLRDQLLSGPLAVRKAVDYGIQVAHGLAAAHEKGIAHRDLKPENLFLTKDGRIKILD
                     FGLAKLVPNPHAAEPGSPTLTEGTEPGVVLGTVGYMAPEQVRGQPADGRADLFALGAI
                     LYEMLAGKRAFHKPTSAETMSAILNEEPSAISQIVPAIPPGLQKVVQRCLEKNPEQRF
                     HSASDLAFALEALSDATSAAPSALAVAKPQSHWKWIAAATTVIALAGALAVWLTRPPA
                     VPVVEAVTQLTDDGEPKMGDLETDGARVYLNEGVAGSLRIAQVSVSGGETVQMPTQLA
                     NPQIVSIRKDGSALLVVVGGAFAHRQPMWLLSLPGGASRRLGDMEVTSASLFPDGRVL
                     YTIGPAVYVAERDGSTPHKLAEVSQPGLDVHWSAVSPDGNRIVLSTFNFVSAKVGPTY
                     EIASDGAGLTEVLGEATGHLLSVTCCARWTPDGRYLLFQTDNQGRSDLWALAEKRQLL
                     RGSPTPVRLTNGPLSYTLGAIGSDGKHIFAIGTKARGELVRYDERTHQFLPYLGGISA
                     FDPTYSRDGKWMAYTSYPDHVLWRSRADGSERLQLTYSPELVFEPRISPDGTRVVFST
                     SDGDAYLISMNGGTPKKIAGNASGADWSPDNNLVVMGSLVPGKLYGEKGAVESRIVDL
                     RSGNISVVPGSEGIWGAWFVAQDTLVAATEDSLRFMLFDFKTRKWTELATDPGTYQAW
                     APSLDGKYLYCTTAGADPKAQRIRIADHAVETITSIKGLRSVSDPSEGALINVAPDGS
                     ALFTRDVGTQEVYAISVKWP"
BASE COUNT          879 a         1375 c         1333 g          903 t
ORIGIN      
        1 gcgaagattt ctgaagacga aagttccgtc tccagctttt ccacaatttc atcgatgtgg
       61 gtagcttcgt cggccttgag caggccgagg attcgctttt cgtgaggagg aagcccttct
      121 tccggaaata gagatgcaga ggaggagccc ggggattcag tcgaagaggc gggagtcagc
      181 gcaagtcgta catctgccgg gagatcctcc cacacgtctt cccaggtcgc ggtcagcttg
      241 gcgccctgct tgatcagtgt gttcggtccc caggaattct tgttggtaac gttgccggga
      301 acagcgaaaa cgtcgcgatt ctgctccagc gcgcaacggg ccgtgatgcg cgtgccggag
      361 tattccgcag cctcgactac gagcacccca aacgacatgc cgctcaggat ccggttgcgg
      421 atgggaaaat tctgcggagc ggcgaacgtg cccagaggaa attcggagat caacgccccg
      481 ccgagggcga gcatctgctc cgacagccgc gagttttctt tggggtagat cacgtccacg
      541 cccgtgccaa acaccgcgat ggtcttgcct ttggccgaga tcgcgccccg gtgcgcggcc
      601 gtatcgacgc cgcgcgccat gccgctgatg atgaccagcc cctgcattgc gagatcgcag
      661 gccagccgtt cggccattcc cgtgccgtaa ggcgtgggat ggcgcgttcc caccatggca
      721 atcccgggtt tggtcagtat ctcaggattg ccacgaatat aaaggactag cgggggatcg
      781 tagatttctt tcagccgggg cggataagcg ggatcatcca tcgagatcac agccacgccc
      841 acagcggccg cccgcgcgat ctcgtcgcgc gccagctcgg ccgacctgcc cgtggccagt
      901 gattgcgcag aaacggcctg aataccggtg ctctccaact ctgtgagaga agtgcggaaa
      961 accgcttccg gagctccaaa gtgctcgacg agctttctgg ctttggtcgg gcccagtccc
     1021 ggggtcaggg caatggccag ccactccagc aggtgggtcg cgcccggcga tgctgagact
     1081 gtcgtcaaga ttgcgctccg cggcggactc tactttcctc tgaacaatgg agtcaaggtc
     1141 atccgtcaca atcggtttgt taatattgtg gaaagaatcg aatcggcatg agactacgta
     1201 tttcggccat ttcctatctc aataccgctc ccctgatgtg ggacttcgag cacggtgacg
     1261 ccggacgaca gttcgacatc tcgtacacgc tgccgtcggc atgcgcgcgg gcgctggcgg
     1321 agggagcggc cgacatcggg atcattcctg cggcggccta tacccagatt ccggggttgc
     1381 agatactgcc cgatgtcacg atcgcctcgc ggcgagccgt gcgctcgatt ctgctggtca
     1441 gcaagcagcc gatcgagaaa gtccgcacgg tggccctcga tacgtcgtcg ctcacatcag
     1501 ttgcgctgac caaggtcttg tttgagagat ggctgggcgg cgcgcggacc tacactccga
     1561 tggcgcccga tatcgaacag atgctggccg aatgcgacgc cgggcttgtg atcggcgatc
     1621 cggcgctgaa gattgattgt tcgcggtacc tgacgctcga tctgtctgaa gaatggatcc
     1681 gccacaccgg caagccgttt gtcttcgcct tctgggcggt ccgtggcggg gctttacgcg
     1741 aggccgattc cgccctggat ttgcccacaa tatttcgtga gtcccgcgac aatgggctga
     1801 cgcccgccag cctcaatcag accgcccgcg agtgggcgcc gcgatttggt ttgacggagg
     1861 ccgaggtcca ttcctatctc accgaaaaca tccactacca actggactcc ggctgtctgg
     1921 aaggtctgca gttgttctat cgttatgccg cggagattgg agccttacct gcggctccgg
     1981 acttgcactt cgtcgaagcc gcgaaagcca tcgcgaccta gaacttctct tctcacggcc
     2041 acttcaccga aatcgcatac acttcctgcg tgcccacatc gcgcgtgaag agcgcggagc
     2101 cgtccggtgc cacattgatc aaagcaccct cggacggatc gctcacacta cgcaggccct
     2161 tgatactcgt tatagtctcc actgcatgat ccgcaatccg gatgcgctgg gctttcgggt
     2221 cagcacccgc ggttgtgcag taaaggtatt tcccgtcgag tgacggtgcc caggcctggt
     2281 acgtgcctgg atctgtagcc agttcagtcc actttcttgt cttgaaatca aagagcatga
     2341 atctcaagga atcctcggtc gcagccacaa gggtatcttg tgcaacaaac cacgcccccc
     2401 atatgccctc ggaaccagga acaacagaga tgtttccact gcggagatcg acaattcgcg
     2461 actcgacggc tcccttctcc ccgtacaatt ttcctggaac cagagaaccc atcaccacaa
     2521 ggttgttgtc aggcgaccag tccgcacccg aagcgttgcc cgcgattttc ttcggggtgc
     2581 cgccgttcat gctgattagg taagcgtctc cgtcggaggt gctaaacacg actctggtcc
     2641 cgtcggggga aatgcgtggt tcaaacacca gttccgggga gtatgtaagc tgcaaccgtt
     2701 cgctgccatc ggcgcgactt cgccagagca catggtcagg ataagaggtg taggccatcc
     2761 actttccatc gcgagaatat gtcgggtcga acgcagagat gcccccgagg taaggcagga
     2821 actgatgcgt gcgctcgtca tagcgaacca gttcgccccg cgcctttgtt ccgatggcga
     2881 agatgtgctt tccatcgctg ccgatcgctc cgagggtata agagagcggc ccgttggtaa
     2941 gcctgaccgg agtgggagag ccacgcagga gctgccgttt ttctgccaga gcccacaggt
     3001 ccgacctccc ctgattgtcg gtctgaaaca gaagataccg gccgtccggc gtccacctcg
     3061 cgcagcatgt caccgagagg agatggccgg ttgcttcccc gaggacctca gtcagccccg
     3121 ctccatccga ggcaatctcg tacgtcggcc caaccttggc cgagacaaag ttaaatgtgg
     3181 aaagaacaat ccggtttcca tccggcgaaa ctgccgacca atgcacatca agtccgggct
     3241 gggacacttc cgccaattta tggggggttg atccgtcccg ttctgcaacg tagaccgcgg
     3301 ggccgatggt gtagaggacg cgaccgtcgg gaaagaggct ggcgctggtc acctccatgt
     3361 cacccaggcg acgcgacgcg ccacctggaa gtgacagcaa ccacatgggt tggcgatgag
     3421 caaacgcccc gcccacaaca acaagcaggg ccgatccgtc tttccgaatc gagactatct
     3481 gagggttcgc cagctgtgtg ggcatctgca ccgtttctcc gccgctcacg gagacttggg
     3541 caatccgtag actgcccgcg acaccctcat tcaggtagac ccgcgcacca tcggtttcca
     3601 ggtcacccat ttttggttcg ccgtcgtcag tgagttgggt taccgcttcg accacgggaa
     3661 ccgcaggcgg acgtgttaac cacaccgcta gagccccagc cagcgcgatc acggtagttg
     3721 cggcagcaat ccacttccaa tgcgactggg gctttgcaac tgcaagagcg ctcggtgctg
     3781 cgctggttgc atctgacaaa gcctcaagcg cgaacgccag atccgacgcc gagtggaaac
     3841 gttgctccgg attcttctcc aggcagcgct gaacgacttt ctgcagcccc ggcggaatcg
     3901 ccggcacaat ctgcgaaatc gcagatggct cttcgttcag gatggcgctc atggtctcgg
     3961 cggatgtcgg cttgtggaac gcacgcttgc cggccagcat ttcgtagagg atcgcgccca
     4021 gggcaaacag gtcggcgcga ccgtccgcag gctggccgcg cacctgctcg ggggccatgt
     4081 agcctacggt gcccaggact actcccggct cagttccttc ggtcagagtg gggcttccag
     4141 gctccgctgc atgaggattg ggaacgagct tggcgagtcc gaaatccaga atcttgattc
     4201 gcccatcctt cgtgaggaac aggttctccg gcttcagatc acggtgggca attcccttct
     4261 cgtgcgccgc tgccagcccg tgcgcgacct ggattccgta gtcgacagct ttgcgaacgg
     4321 cgagcggacc gctcagcaac tggtcgcgca gcgtgctgcc ttcgagcaac tcggagacca
     4381 gatacggcgc tccttcatgc gtgccgaact ggtagacagc aagaatgttg gggtggttga
     4441 gcgcagccgc agcccgagct tcctgttcga accgtcgcag gcgatcggga
//
LOCUS       DASPUL010000542         5631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1875564, whole genome shotgun sequence.
ACCESSION   DASPUL010000542 DASPUL010000000
VERSION     DASPUL010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1875564"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VEJ00_12675"
     CDS             complement(<1..966)
                     /locus_tag="VEJ00_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02062.1"
                     /translation="MTSRPGRWLRDPLILLCLTAGLLAFVVQSGELGSADTTHRLQTT
                     HWLWTSEPQVFPNEYPEFGLPGRGGRLYSWYGIGQSLLMLPADVAGTWMARLQMFSEY
                     QDDPAVRSIVVSYSTNILVNVLTTMVAFRLLGQLRFSVKESAAGVLALLLCTTHLHYT
                     QNMMENNYILLLTLAGFSFQYEWLRSDDVRALFLGSAALGLNLLTRVTTVLDLIAAGV
                     FLLAVLWFENVRGRTLWQRVLAYCKVAVPVYIFFLFAERWYNFYRFGSWTKTYLPLFA
                     QVQRMQDPNLPPAFPWSTPFHVGFLGALFKPEKSIFLFDPLLVLAL"
     gene            1301..3835
                     /locus_tag="VEJ00_12680"
     CDS             1301..3835
                     /locus_tag="VEJ00_12680"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXX02063.1"
                     /translation="MLSEGRFLPGRLVAGRYRIIALLGKGGMGEVYRADDLTLGQPVA
                     LKFLPDEAAHDEALLERFRNEVRIARRVSHPNVCRVYDVGEVDGHTFFTMEYVDGEDL
                     ASLLRRIGRLPQDKALEIGRQLCAGLAAAHSKGVLHRDLKPANIMLDGRGQAVIMDFG
                     LAGIADDIRGAEVRSGTPAYMAPEQLAGKEVSMRSDIYSLGLVLYEVFTGKRAFAESP
                     AQALRSRGDRTPSRPSSVVKDLDPIIEKVILRCLETEPSARPATALAVAAAMPGGDPL
                     AAALAAGETPSPQMVAAAGETVGLRPRVAVICFASLLAALGCVFYLSVHSSGLDKLHL
                     EQTPEVLAQKARETAAGLGYPERPADSAFSLDYDIDFLNFVEKNDKPHPNWDAVLAGR
                     PSPLFLWYRQSPDTMVAEGFPDQFLNPGIVTPTNPPTVLSGMVNLQLDPQGRLTYFQA
                     IPAQKENPAGSAALPDWNVLFSAAGLDPAQLQKAEPQWNSLAAFDSRAAWTGTWPGTS
                     RPLRVEAAAYKGKPVFFSLIGDWTKTDRMKAPEQTRSQKIQQIVGIVLTLALFLGASR
                     LAHVNYRRGSSDRQGAFRLAVIMFALQMLLWLCRTHIVANAAMLYLLVLAVSTSMFAA
                     GTTWMLYLAIEPWIRRRWPQAIISWSRLLSGQFRDPLVGRDILFGVMLGVVWILIIQV
                     RAIPMMHMGASPNLLDTEYLMGGREALGAWLKQIPASIFATLEFFILLFGLRFVLRKD
                     WLAAIAFVAVFVVQRFSGPYAAVELPAWILVYAVAVLIVFRFGLIPLAIAIFTVDMLA
                     NVPLTADFSAWYMGTTALCLLSVVALAGWGFYHSLGGAPVWKVEMD"
     gene            4024..>5631
                     /gene="dnaK"
                     /locus_tag="VEJ00_12685"
     CDS             4024..>5631
                     /gene="dnaK"
                     /locus_tag="VEJ00_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXX02064.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGEPKVIANEEGGRTTPSVVGFTK
                     TGERLVGQVAKRQAITNPENTVYSIKRFMGRRFNEVTEEMKMVPFKVVQSGDHVAVLA
                     QGKEYTPPQVSAMILQKLKKAAEDYLGEKVTEAVITVPAYFNDAQRQATKDAGQIAGL
                     EVKRIINEPTAAALAYGLDKGKDETIAVYDFGGGTFDISILEVGEGVIEVKATNGDTH
                     LGGDNIDQRLVDWLIDEFKKDEGLDLRGKGNEMALQRLRDAAERAKIELSTTMETEIN
                     LPFITADASGPKHLVKKLTRAKLESMVEDIIQKSVGPCKQCMKDAGIDASKINEVVLV
                     GGQTRMPRIQQLVKELFGREGHKGVNPDEVVAVGAAIQGGVLKGEVKDLLLLDVTPLT
                     LAIETLGGVATPMIPRNTTIPTKKTETFSTAADSQTSVEVHVLQGERPMAAQNRTLGK
                     FHLTGIPPAPRGVPQIEVTFDIDANGILNVTAKDTATQKDQKITITSSSGLSKDEVER
                     MAKEADAHSAEDKAKRDEIEARNQLDSM"
BASE COUNT         1160 a         1619 c         1817 g         1035 t
ORIGIN      
        1 aagtgccagc accagcagcg ggtcgaaaag gaagatcgat ttctccggct tgaacagcgc
       61 gcccagaaat ccgacgtgaa acggagtgct ccacgggaag gcagggggca agttaggatc
      121 ctgcatgcgc tgcacttgcg cgaaaagcgg aagataggtc tttgtccacg atccgaagcg
      181 gtagaagttg taccagcgtt cggcgaagag aaaaaagatg tacacaggaa cagctacctt
      241 gcaataagcc aaaacgcgct gccacagggt gcgcccgcgc acgttctcaa accaaagaac
      301 tgcgagcaag aacacgccgg ctgcaatcag atcgagaacg gtggtgaccc gcgtcagcag
      361 gttgagaccc agcgcggcag atcccagaaa gagcgcgcgg acatcatccg atcttagcca
      421 ctcgtactga aaagagaatc cggccagagt cagcagcagg atgtagttgt tctccatcat
      481 gttctgggtg tagtgcaggt gcgtggtgca caaaagcagg gccagcacac ccgctgccga
      541 ctccttcacg ctaaagcgga gctggcccag gaggcgaaac gcgaccattg tcgtcagaac
      601 gttcaccaaa atgttggtgc tgtagctgac cacaatgctg cgaacggcgg ggtcgtcctg
      661 atattcagaa aacatctgca ggcgagccat ccacgtgccc gcgacatccg cgggcagcat
      721 gagcagcgac tggccaatgc cataccagct gtaaagtctg cctccccggc cgggcaggcc
      781 aaattcgggg tattcgttgg gaaatacctg gggctcggac gtccaaagcc agtgggtagt
      841 ctgcaggcgg tgggtggtgt cggcgctgcc caattcaccc gactgcacca cgaacgccag
      901 caagcccgcg gtcagacaca ggaggattag aggatcgcgc aaccagcgcc ccgggcgtga
      961 cgtcattgca tggatggtaa tacagaatcg gcttctggct tccggcggcc ggctcccagc
     1021 ttgcgtagct gcgcggccgc attctcgcgc gacgcgagtc cggaagccgg aagccggatg
     1081 gcgtctagag tagactgtag cgggcaaatt catggcttcg tgttcaactt gcggagcgga
     1141 gttggcgagc ggcgtacgct tctgcccttc ctgcggacgg cccacggtga acgcgcacga
     1201 cgtggcgacg cttgactatg cggcccccac agcgacttcg cctcttcctc ctaaaccacc
     1261 ctcggcttct tcatcgcgac ctcctagctc agccggagcc ttgctcagtg aaggacgatt
     1321 cctccccggg cggctggtgg ccgggcgcta ccggatcatc gccctgctgg gcaagggcgg
     1381 gatgggcgag gtctatcgcg ccgacgacct cacgcttggg caaccggtcg cgctgaagtt
     1441 tctgccagat gaggccgcgc acgacgaagc gctgctcgag cgttttcgca acgaagtgcg
     1501 cattgcccgc cgcgtttcgc accccaacgt ctgccgcgta tacgacgtcg gtgaagtcga
     1561 cggccacacg ttcttcacca tggaatacgt cgacggcgag gatctggctt ccctgctgcg
     1621 gcgcatcggg cggctgccgc aggacaaggc gctcgaaatc gggcgtcagc tctgcgccgg
     1681 gctggcggcg gctcattcca agggcgtgct gcaccgcgat ctgaagccgg ccaatatcat
     1741 gctggacggg cgcgggcaag ccgtaatcat ggatttcggc ttggccggca tcgcggacga
     1801 cattcgcggg gccgaggtgc gcagcggcac tcccgcctat atggcgccgg agcaactggc
     1861 gggcaaggaa gtcagcatgc gcagcgacat ttattcgctc ggcctggtgc tgtatgaggt
     1921 attcacgggc aagcgggcgt ttgcggagtc tccggcccag gcactgcgct cgcgcggcga
     1981 tcgcacgccg agccggccgt cgtctgtcgt gaaagatctc gatccgatca tcgagaaagt
     2041 aatcctccgg tgcctggaga ccgaaccttc agctcgtccc gccacggctc tagccgtggc
     2101 agccgcgatg cccgggggcg atccgctggc ggcggcgctg gcggcgggag aaaccccatc
     2161 gccgcaaatg gtcgcggccg ccggagagac ggtggggctt cgcccgcgcg ttgcagtgat
     2221 atgcttcgca tctcttctcg ccgcgctggg atgcgtgttc tatctgtcgg ttcacagcag
     2281 cggactggac aagctgcatc tggaacagac gccggaggta ttggcgcaga aagcgcgcga
     2341 gacagcggcc ggcctgggat atcctgagcg gccggcagac agcgcgttca gtttggacta
     2401 cgacattgat ttcctgaatt tcgtcgagaa gaatgacaag ccgcatccca actgggatgc
     2461 cgtgcttgcc ggccggcctt cgccgctgtt cttgtggtac cggcagagtc ccgacaccat
     2521 ggtcgctgag ggattccctg atcagtttct caatcccggc atcgtcacgc cgactaatcc
     2581 accgacggtt ttgtcaggga tggtgaatct gcaactcgat ccccagggtc ggttgaccta
     2641 ttttcaggcc atcccagcgc agaaggagaa tccagccggc tcggcggcgc ttcccgactg
     2701 gaacgtgctg ttctctgcgg cagggctcga cccggcgcaa ttgcaaaagg ccgagccgca
     2761 gtggaactct ctggctgcgt tcgattcccg ggcggcctgg accggaacct ggccaggaac
     2821 gtcgcgaccg ctacgagttg aggccgctgc ttacaagggg aagccggttt tcttctcgct
     2881 catcggtgat tggactaaga ccgaccgaat gaaagcgcca gagcaaacca ggagtcagaa
     2941 gatacagcaa atcgtcggaa tcgtgctgac gctggccctg ttcctgggag ccagccggct
     3001 ggcgcacgtc aactaccggc gggggagcag cgatcggcaa ggcgctttcc gtctggctgt
     3061 gatcatgttt gccctgcaaa tgctgctttg gctgtgccgc acccacattg tcgccaatgc
     3121 cgccatgctt tacctgctcg tgctcgcggt cagcacgtcg atgtttgccg caggcacgac
     3181 gtggatgttg tatctggcga ttgaaccgtg gatcagaagg cgctggccgc aggccattat
     3241 ttcgtggagc cggctgctgt cagggcagtt ccgcgatccg ctggtagggc gcgacatcct
     3301 ctttggggta atgttgggcg tggtgtggat tctgattatc caggtccgcg ccatccccat
     3361 gatgcacatg ggcgcatcgc ccaatctgct ggacaccgag tatctgatgg gcgggcgcga
     3421 ggccctgggg gcatggctga agcagatacc ggcgtcgatt tttgcaacgc tcgaattctt
     3481 tatccttttg ttcgggctga gattcgtgct gcgcaaggat tggctggcgg ccattgcgtt
     3541 tgtggccgtc tttgtagtgc aacgcttctc gggcccatac gccgccgtgg agctgccggc
     3601 ctggatcctg gtgtatgccg tcgcggtgct gattgtattc cgcttcggac tgatcccgtt
     3661 ggcgatcgcc atcttcaccg tcgacatgct ggccaacgtg ccgctgacgg cagacttctc
     3721 ggcgtggtat atgggcacga ctgcgctctg ccttctgagc gtggtcgcac tggccggatg
     3781 ggggttctat cactccttgg gcggagctcc ggtgtggaag gtggagatgg actagggccg
     3841 aagcggcgtc aggcaagccg tttaactctc gcccaaatcc ctcgcatcct cttgactctc
     3901 cgggacatct aaaacattga caatggttca ctcaagtctt accatgtgcc tgatataaaa
     3961 gcacttcagc gcaactattt attgtcagca aaggacttat ctggatttta ggaggagcag
     4021 tttatgggga aaattattgg aattgacctg gggacaacca attcctgcgt ggcagtcatg
     4081 gagggcggcg agcccaaggt gatcgccaat gaagaaggag ggcgcacaac gccctcggtg
     4141 gtcggtttca ccaagaccgg ggaacggctg gtagggcagg tcgcaaagcg gcaggccatc
     4201 acgaatcccg agaacaccgt gtattcgatc aagcggttta tgggacggcg cttcaacgaa
     4261 gtcaccgaag aaatgaagat ggtgccgttc aaggtcgtgc agtcgggcga ccacgtcgcc
     4321 gtgctggcgc agggcaagga gtatacgccg ccgcaggtgt cggcgatgat cctgcagaag
     4381 ctgaagaagg cggcggagga ctacctcggc gagaaagtga ccgaagcggt cattacggtg
     4441 ccggcgtact tcaacgatgc ccagcggcag gcgaccaagg atgccggcca gattgccggg
     4501 ctggaagtga agcgcattat caatgagcct acggcggccg cgctggccta cgggctcgac
     4561 aagggtaagg acgagaccat cgccgtctat gacttcggtg gtggcacgtt cgacatttcg
     4621 attctcgaag tcggcgaggg cgtgattgaa gtcaaagcca ccaatggcga cacgcacctg
     4681 ggcggtgaca acatcgacca gcggctggtg gattggctga tcgacgagtt caagaaggac
     4741 gaagggcttg acctgcgcgg caagggcaat gagatggctt tgcagcgtct gcgcgacgcg
     4801 gccgaacggg ccaagatcga gctctcgacc acgatggaga ccgagatcaa tctgccgttc
     4861 atcacggcgg atgcgagcgg gccgaagcac ctggtcaaga aactgacccg ggccaagctc
     4921 gagtcgatgg tggaagacat catccagaag tcggttggcc cgtgcaagca gtgcatgaaa
     4981 gacgcgggga ttgacgccag caagatcaac gaagtcgtgc tggtgggcgg acagacgcgc
     5041 atgccgcgca ttcagcagct cgtgaaggag ttgtttggac gcgaagggca caagggcgtg
     5101 aatcctgatg aggttgttgc agtcggcgcg gcgattcagg gcggcgtgct caagggtgaa
     5161 gtgaaagacc tgctgctgct tgacgtgacg ccactgacgc tggcgattga aacgctgggc
     5221 ggcgtggcga cgccgatgat cccgcgcaac acgacgattc cgaccaagaa gacggaaacg
     5281 ttttcaaccg cggccgacag ccagacctcg gttgaagtgc acgtgctgca gggcgagcgg
     5341 cccatggcgg cgcagaaccg gacactgggc aagttccacc taaccggaat tccaccggct
     5401 ccgcgcgggg taccgcagat cgaagtgacg ttcgacatcg acgccaacgg catcctgaac
     5461 gtgaccgcga aagacacggc cacgcagaaa gaccagaaga tcacgattac gtcgtcttcc
     5521 ggcctgtcaa aggacgaagt cgagcggatg gccaaggaag ccgacgcaca ctcggccgaa
     5581 gacaaggcca agcgcgacga gatcgaggcg cgaaaccagc tcgactcgat g
//
LOCUS       DASPUL010000543         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2022606, whole genome shotgun sequence.
ACCESSION   DASPUL010000543 DASPUL010000000
VERSION     DASPUL010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2022606"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2249)
                     /locus_tag="VEJ00_12690"
     CDS             complement(<1..2249)
                     /locus_tag="VEJ00_12690"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXX02065.1"
                     /translation="MPTKIGHFEILSELAKSPTGTVYKANDPESGQTIALKAIQLSAF
                     GEHAKALEQALLQEVENTKTLSSPNITPVFGAGEIEGQFCAAMEYVQGNSIATMLTRK
                     EGFSIWDLLDIGRQLCAGLDYAASKGVVHYSLEPAKIMCGWDGTVKILAFGVSSVGKF
                     SHQVEEGLPSSLHYMSPEQVMDQPTDGRSNLFSLGAIFYEMVTERKAFDRDDIQNLRQ
                     SILDFTPVAPAHVNPKVHPLLSDLIMKALSKDPAQRYQTGRDLLDDLEKCKESKPVAA
                     PKKPAATQGTVVPGAAKAAAQAKFVGQSPARKPANPASPAPPVAAKPVSQSGLARPST
                     PAPSARTENSAAAKPASQPAGTKFAEPPAPRPEAPPSKLAVPKSGLAMPKAAAAAAGV
                     GSGSQATSSSAEIPEIDLSNQFITSCVKATIEAAEQPSESMSSAVADEPEVETFEPES
                     SSAPRIAVDPLMAEGEGGASAGTSFSDISELPPLKEVYVEPPPPPPVFQPTPAPTRSS
                     TTFDGKLKREEKPKVQPKEVAEKAIKEIKSVPPSLFAYSIGIALAIIVAIGIGVTIYV
                     HSQNSDDDAGAARSSATTSAAPVAESNQAAPQTTPAAAAAAAPAPVEGSAPEPAPAQP
                     VAAARARSPHKKAAPAAPAIIPGSLAIDSTPPGAQVQLDGQSDASWVTPLALTSLQPG
                     QHMITVSKSGYSSDSRTINVTSGNRATVVIHLAQLMATLVVKSDPPGANIYIDGRDMG
                     AKTPAQVSV"
BASE COUNT          430 a          765 c          868 g          546 t
ORIGIN      
        1 tcgacgctta cctgagccgg agttttcgca cccatgtcgc ggccgtctat atagatgttg
       61 gcgccgggag gatcactctt gacgacgaga gtggccatca gctgggccag atgaatgacg
      121 accgtcgctc ggttgccgga agtcacattg atggtccgtg aatcgctgct gtagccggac
      181 ttgctgacgg tgatcatgtg ctgccccggc tgcaggctgg tgagcgcgag cggcgtgacc
      241 cagctcgcgt cactttggcc atccagttgt acctgtgccc cgggcggtgt ggaatcaatc
      301 gccaaactgc caggaataat tgcgggtgcc gccggagccg ccttcttatg gggacttcgg
      361 gctcttgccg cagcgactgg ctgagccgga gcgggctcag gagcagaacc ctccaccgga
      421 gcaggcgcag ccgctgctgc tgcggctgga gtggtttgcg gtgcagcctg gttggactcg
      481 gccacaggtg ctgcggatgt ggtcgcggac gaccgggcgg cgcccgcgtc gtcatcgctg
      541 ttctgactgt gaacgtaaat cgtcacgccg atgccgatcg cgacgatgat cgctagcgca
      601 atgccaatcg agtaggcgaa gagactgggc ggaacgctct tgatctcctt gatcgccttc
      661 tcagctactt ccttgggctg caccttcggc ttttcttcgc gcttcagctt gccgtcgaaa
      721 gtcgtggagg agcgagttgg ggccggggtc ggctgaaaga cgggtggtgg cggaggcggc
      781 tcgacataga cttctttgag cggcggcagt tcggagatgt cggagaagct ggtgccggcg
      841 ctcgcgcccc cttcaccttc agccatcagc ggatcgacgg cgatgcgcgg agcactggac
      901 gattccggct cgaaggtttc aacttcaggt tcgtcggcca cggcagaaga catggactcg
      961 gatggctgct ccgctgcttc gatcgttgcc ttgacacagg aggtgatgaa ctggttcgag
     1021 agatcgattt ccgggatttc cgcggaagac gaagttgcct ggctgccgct gcccacaccc
     1081 gccgcggcag cagccgcttt cggcatggcg aggcccgact tcggcacggc cagcttggaa
     1141 ggcggggctt ccggcctagg cgcgggtggt tcggcgaatt tcgttcccgc cggttgactt
     1201 gccggcttgg cagctgcgga gttctccgtg cgtgccgacg gcgccggagt ggaaggccgc
     1261 gccaggcccg attgcgacac tggtttcgct gcaactggtg gcgcggggct ggccgggttc
     1321 gcaggcttcc tcgccggtga ctgccccaca aacttcgctt gcgcagccgc cttagccgcg
     1381 cccggaacca ccgtgccctg cgtcgcagcc ggtttcttgg gcgcggccac gggcttcgat
     1441 tccttgcatt tctcaaggtc gtcgagcagg tcacggccgg tctggtagcg ctgtgccgga
     1501 tccttcgaga gcgccttcat gatcaggtcg ctgagcagag gatgcacttt gggattcaca
     1561 tgagccggcg ctacaggcgt gaagtcgaga atgctctggc gcagattctg gatgtcgtcg
     1621 cgatcgaacg ccttgcgctc ggtcaccatc tcgtagaaga tggcgcccag gctgaacaga
     1681 ttggaacggc cgtcggttgg ctggtccatc acctgctcgg gcgacatgta gtgcaggctg
     1741 gacggcagtc cttcttcgac ctggtgcgag aacttgccta cgctggacac gccaaaagcg
     1801 aggatcttga ccgtgccgtc ccagccgcac atgattttcg ccggctcgag gctgtaatga
     1861 accacgcctt ttgacgcggc gtaatcgagg ccggcgcaga gctggcgtcc tatatcaaga
     1921 aggtcccaga tggagaagcc ttccttgcgg gtcagcatgg tggcgatgct gttgccctgc
     1981 acgtattcca tggcggcgca gaactggcct tcaatttcgc ccgcgccgaa aaccggagtg
     2041 atgttgggac tgctgagagt cttggtgttt tctacttcct gcagcagagc ctgctcgaga
     2101 gccttggcat gctctccgaa ggcgctgagc tgaattgcct tcaaggcaat ggtctgcccg
     2161 ctctccgggt cattggcttt gtagaccgtg ccagtcggtg atttcgccag ctcactgaga
     2221 atctcgaagt ggccgatttt cgtgggcatt tccgttccag ctcccttcga tcgcgaacct
     2281 tcggcgtggc ccatcacggc tcgccggggg agattcgagt gcagggtatc gctaggatac
     2341 cgctgctcga tgcgggcttc gagttaccaa ggtaagtggg gaagtccaag cctggcgcgg
     2401 agttgcgggg cgcgatcggg aagaagaggg gtgtctatcg agatcggaaa gcagggactt
     2461 acccgccctg ccgcgcaggg cagagcaacg tggcagggcg cgctgaattt cggcgccacc
     2521 ggattgcctt tagcttagtt ccacggcaaa gctgccgctg gttgcgtcgc gatctgaacc
     2581 cggttccgcc acccaacgcc aggctctcc
//
LOCUS       DASPUL010000544         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_780537, whole genome shotgun sequence.
ACCESSION   DASPUL010000544 DASPUL010000000
VERSION     DASPUL010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780537"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VEJ00_12695"
     CDS             <1..281
                     /locus_tag="VEJ00_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02066.1"
                     /translation="RSFSYSNFVGAFTSGGLSNLYYPGRAVVRTAPATATAPPMPVFE
                     DDRGVVLTVSRSIIALGYDMAGNLFDEFWPDIRRKFKGKHNPESGSKP"
     gene            complement(410..976)
                     /locus_tag="VEJ00_12700"
     CDS             complement(410..976)
                     /locus_tag="VEJ00_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02067.1"
                     /translation="MSITTLIILVVLALAVGLFVIRVIPGVRAYFDYRGKRLITCPES
                     HTPEAVEVAAREAGMGAFLNEPVLRLTECSRWPERQDCGQECLKQIEMDPARCLVWNI
                     VAKWYEGKSCVFCHKQIGPLHHLDHVPALLAPDHITTEWKRIRPEKLPEVFETYRPVC
                     WNCHVTETFRRLHPDLVVEREPEPKRMR"
     gene            1097..1558
                     /locus_tag="VEJ00_12705"
     CDS             1097..1558
                     /locus_tag="VEJ00_12705"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HXX02068.1"
                     /translation="MIGVTWSIGGNAASQARRTGGSKTMQKMIFLQAALNGDRIHPAA
                     PRIPAAIADAARAAVNAGAHSVHVHAFDDAGREILDSAACAKVLRAIRAFCPGTPISL
                     TTSAAIVADPAKRLWDLSSLWRSALSTICDRFHEGCRGGDRARWDTDPAAG"
     gene            complement(1854..>2528)
                     /locus_tag="VEJ00_12710"
     CDS             complement(1854..>2528)
                     /locus_tag="VEJ00_12710"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HXX02069.1"
                     /translation="DKYGIPIVEDDPYGQLRYEGEHIAPLVALDRENLRRDNGFTLGN
                     VIYLSTFSKTLAPGIRLAWIVAPEDVISKLVLLKQAADLHTSTFNQYVAYEVARDGFL
                     DQHVKLIRQVYRERRDAMLHSLEEYFPPEVTWTHPKGGLFLWVTMPEGTDSQALFHAA
                     LAENVAFVAGDSFYATENHEGSRHMRLNFSHSDPELIREGIRRLSVAVKMQLRSTHHA
                     SPVAGS"
BASE COUNT          500 a          719 c          796 g          513 t
ORIGIN      
        1 agcggtcgtt cagctattcc aatttcgtcg gagccttcac cagcggaggt ttgtcgaacc
       61 tgtactaccc gggaagggcg gttgtacgca cggctccggc gacggcgaca gcgccgccga
      121 tgcctgtctt tgaagacgat cgcggagttg tgctcacggt gagccgctcc atcatagcgt
      181 tgggctacga tatggcggga aatctgttcg acgaattctg gcctgacatc cgccgcaagt
      241 tcaagggtaa gcacaaccca gagtcgggga gcaaacctta gacgcctgcg gaggagatcc
      301 gcgggtgaag ggacgctggc ccgcgagttg ttgattcttg aatgttgatt gttgaatgaa
      361 aacccaaacc cttatttagc aggtttcaat caacaatcaa caggtctttc taccgcatcc
      421 gctttggctc cggctcgcgc tccacgacca ggtccggatg caggcggcgg aaggtttccg
      481 ttacgtggca gttccagcac accggccggt aagtctcaaa gacttcgggc agtttctcag
      541 gccggattcg cttccattcg gtcgtgatgt gatcgggcgc gagcagagcc ggaacgtgat
      601 ccagatgatg caacgggccg atttgtttgt ggcaaaacac gcagctcttc ccttcgtacc
      661 acttcgcaac gatgttccag accaggcacc gggcggggtc catctcgatt tgcttgagac
      721 attcctgtcc gcagtcctgg cgttcgggcc agcgtgagca ctcagtaagg cggagaacgg
      781 gctcgttcag gaaggcgccc atcccggcct ctcttgccgc gacctcgacg gcttccggcg
      841 tatgcgattc gggacaggtg atcagccgtt ttccgcggta gtcgaaatag gcccgcacgc
      901 ctgggatgac acgaatcacg aacagaccta cggccaaggc gaggacgaca aggatgataa
      961 gggtcgtgat cgacatagat cacctcccgg ccggcaagcc tcccttcgcc gaacggcgcg
     1021 aggccaccgg accatcgtgc aaattcagtg tgaccggaag cacgcaaaag agcggtgccg
     1081 ggcatcacga gaacaggtga tcggcgtcac atggagtatc ggcggcaatg ccgccagcca
     1141 agctcgccgc accggaggat cgaagacaat gcagaagatg attttcctgc aggctgcgct
     1201 gaatggcgac cggattcatc cggctgcacc gcgcattccc gctgctattg cggatgcggc
     1261 tcgtgcggcc gtgaacgccg gcgctcattc cgtgcatgtt catgccttcg acgacgctgg
     1321 tcgggagatt ctcgacagcg cggcttgcgc caaggttctg cgcgcaatcc gggctttctg
     1381 cccgggcact ccgatttcgt tgacaacgtc agcggcgatt gtggcggatc cagcaaagcg
     1441 tttgtgggat ttgtcgagcc tgtggcgcag cgccctttcg actatctgcg atcgttttca
     1501 cgaaggatgc cggggaggcg atcgtgctcg ttgggataca gatccggcgg cggggtaatc
     1561 ccttttgatg cgacctgata ccttgcgcag gaaaacttgt cgctccggca gtatttctcg
     1621 atgagctgct tggcggttgt gggcaaacag ctcaggatct ggttaaagaa tggacacttg
     1681 ctaagtctct cgcaatccgc catggcgcgc taaccccaag gcgaacatcg gcacagaccc
     1741 gctgcaactt caattagaac cttcagagaa tgatcgccac gtgcccgatg gcacgcgcca
     1801 ggcaaaaccc tgacgtgcct tcgcggccgt gcccaaagct gtccggagcg gcgctagctc
     1861 cctgcgaccg gagaggcgtg atgggtgctt cgcagctgca tcttcaccgc gaccgagagc
     1921 cggcgaattc cctcgcggat cagctcgggg tctgagtgcg agaagttcag ccgcatgtgg
     1981 cggctgccct cgtggttttc ggtggcgtag aagctgtcgc cggcgacaaa ggcgacgttc
     2041 tcggccagag cggcgtggaa cagcgcctgc gaatcggtgc cttccggcat cgtgacccag
     2101 aggaacaagc cgcccttggg atgagtccac gtgacttctg gtgggaagta ttcttcgagc
     2161 gagtgcagca tggcgtcgcg gcgctcgcgg tagacctggc gaatcagctt gacgtgctga
     2221 tcgaggaagc catcgcgggc gacctcgtaa gcgacatatt gattgaacgt ggaagtatgc
     2281 aggtcggctg cctgcttgag caacaccagc ttgctgatca cgtcttcggg agcgacgatc
     2341 caggccaggc gaattcccgg tgctagggtc ttcgagaacg tgctcagata gatgacattg
     2401 ccgagggtga agccgttgtc gcggcggaga ttctcgcgat cgagagcgac caggggcgcg
     2461 atgtgctcgc cttcgtagcg gagctggccg tagggatcat cttcgacaat ggggatgccg
     2521 tatttgtc
//
LOCUS       DASPUL010000545         7112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1876016, whole genome shotgun sequence.
ACCESSION   DASPUL010000545 DASPUL010000000
VERSION     DASPUL010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1876016"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2124)
                     /gene="mnmG"
                     /locus_tag="VEJ00_12715"
     CDS             complement(<1..2124)
                     /gene="mnmG"
                     /locus_tag="VEJ00_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HXX02070.1"
                     /translation="MKAVKNVTNLGHAPVLMHFTEQFDVVVVGAGHAGCEAAMASARM
                     RLKTALYTLNVDLIAQMSCNPAVGGIAKGHLVREVDALGGIMGEITDAVGIQFRLLNT
                     SRGPAVWSPRAQCDKQAYRLKMREVLESQANLKIKQAEVADLIVEPAPGSQLPAPGQA
                     REQHSAAVSGRRGPDARSRVIGVKLRDGRTVGAQAVIITTGTFLNGLIHCGEQQYPAG
                     RSGEPNAVLLGEALKKLGLRGCRLKTGTPPRLDGRSIEWSRFPVQPGDDDPTPFSFRT
                     RRVAHHDAQVPCYIAWTTAETHRIIRQNVHRSPMYSGQIQSIGPRYCPSIEDKIVKFP
                     DKETHQLFLEPEGKNTHEIYVNGMSTSLPVDVQLAILKSIPGLETAEMLRPGYAIEYD
                     SIDPTELERTLETRKIAGLFLAGQINGTSGYEEAACQGIMAGINAALQVKQEPPLILD
                     RTEAYTAILIDDLISKGTNEPYRMFTSRAEFRLHLRIDNADRRLTPHGRRVGLISNEA
                     WADFQAKQARLEKMKKLLEGTRLTPEMLEGMQAAAIGNSHVGAGASPVQAEQSSAGLL
                     PGAAGQSLAQLLKRPEVTIERLVPVLRKLDPEFFESEGIRENPCESAAGISSTLRNEL
                     KSVETEIKYEGYLRQQERSMERLKKAEQHAIPEWFDYRSVSGLSREMQEKLTRVQPRT
                     LGQASRIPGVTPAAVSLVNVYIEIQA"
     gene            2219..2920
                     /locus_tag="VEJ00_12720"
     CDS             2219..2920
                     /locus_tag="VEJ00_12720"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HXX02071.1"
                     /translation="MTTAEFFDQLEARIAKYDLLCHPFYQAWSAGELSREDLREYAQD
                     YYHHVEAFPSYLAAFGLRLEDGELRRAVLANLCDEKGIEGRAGKESVPHSDLWLDFAE
                     GMGSTRNLEWHCPAPEVRQLIRHFHRVANEGLPEEALATFYAYESQVPRIAREKERGL
                     RQNYGADDKTCGYFSLHSTADVHHARVWHTQLEKRIAANPEAADAALDAAEKAARALW
                     HALDGIDARRMAMAA"
     gene            3093..3482
                     /locus_tag="VEJ00_12725"
     CDS             3093..3482
                     /locus_tag="VEJ00_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02072.1"
                     /translation="MARPVPIGVRGETQETVAFEHTLTSHNPKLPPVYSTPDMIRLME
                     IAAFQALQPYCKGDEITVGTSINVEHRAASGIGAHITAEAILESFDGRFYTLRVAARD
                     DAREIGRGTVSRAVVSLGKFADRMKQR"
     gene            complement(3555..4400)
                     /locus_tag="VEJ00_12730"
     CDS             complement(3555..4400)
                     /locus_tag="VEJ00_12730"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HXX02073.1"
                     /translation="MPDPALSGSEFKKQLRAGQPKMGLFLNAHSPTVAEQLAHSGYDW
                     LLVDTQHGPMGYEKLSAMLSGIANGGAKSLVRVGGYDDRPGIQQALDMGADGVLIPYI
                     NTADEARQAVSCTKYPTVGTRSVYFPQRSMNRAGLLGYAGSWNNEGIVALQVETASCI
                     ENIAEIAAVPGVDILFLGQNDLCMSMGLYNKYEFPHMYTSPELDAATKKLIEHARKNN
                     VILGLFLFGTARVGEFLEKGFTFISIGNDLHHVLTQAGAYVADMEKIAQEKGKSWKRR
                     PTALF"
     gene            4599..4952
                     /locus_tag="VEJ00_12735"
     CDS             4599..4952
                     /locus_tag="VEJ00_12735"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXX02074.1"
                     /translation="MRMLLNWVLSAVAVWIVAQLVPGVHVSGPVAALIAALAIGFINA
                     TLGMLLKIITFPLTLLTLGLFWLVINALMLKLASALVPGFQVRGFLAAFIGAIVLSLV
                     NLVLKGIVMPRKDQK"
     gene            complement(4965..5516)
                     /locus_tag="VEJ00_12740"
     CDS             complement(4965..5516)
                     /locus_tag="VEJ00_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02075.1"
                     /translation="MAYTRRKIRGINLSFLLASFVFACWALVCAWFGWPRGFRGGSTA
                     GFGLFAAAALFFGAFPAIWARYPSKHPVNHELLRYGDLAEVSERLDREMQGSVDVVGP
                     FRFTASFLVYDSGHEFQLVPYDQIVSSEVDKVGSDEPSAVVVRTRSGRRYQWYRTWMQ
                     GIFDPEQVSKKIRAAARLDAPAG"
     gene            complement(5517..6371)
                     /locus_tag="VEJ00_12745"
     CDS             complement(5517..6371)
                     /locus_tag="VEJ00_12745"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX02076.1"
                     /translation="MFRADLLQNKRILITGGGTGLGKGMAQRFLELGAVVYICGRREE
                     VLKATAAELSPKGKIHAIPCDVRSLEAVEGMLDSIWKEGALDVLVNNAAGNFIARSEE
                     LSPGAWNSVISIVLMGTLNCTTACGRRWLSGKRAGTVLSISATYAPVGSAYVVPSAVS
                     KAGVEALTRSLAVEWGNRGIRMNAIAPGPIPTQGAFSRLLPRPELEQQALNRNPLHRF
                     GTVEEVANLAAFLVSDGSGYINGEVVRMDGGEFLQGAGEFSNLGRLLREEEWQAMKPK
                     KPGSAPKS"
     gene            complement(6510..>7112)
                     /locus_tag="VEJ00_12750"
     CDS             complement(6510..>7112)
                     /locus_tag="VEJ00_12750"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXX02077.1"
                     /translation="DTFRLAATQVTNAEYERFLRATTPQEPPFWRDANFNHPQQPVTG
                     VSWHEARLYCEWLTSQTGRRYRLPSEAEWERAVRGGLEQKQFPWGDEPPQSLPDYATR
                     WQNGPEPVAQYAPSTFGLYDMGDNVHEWCSDWYDPNYYAVSPERNPRGPERSPMQPQR
                     KASRGGSWRHHIKVARCSARSSIPPEFQYADYGFRVACDL"
BASE COUNT         1306 a         2273 c         2126 g         1407 t
ORIGIN      
        1 cgcctggatt tcaatgtaca cgttcaccag cgacaccgcg gccggtgtta cgccaggaat
       61 acgggaagcc tggcccagcg tgcgcggctg tactcttgtc agcttctcct gcatctcgcg
      121 cgagagtccg ctgacggagc ggtagtcgaa ccactcggga atcgcgtgct gctcggcctt
      181 cttcagtcgc tccatcgagc gctcctgctg gcgcaggtag ccttcatact tgatctcggt
      241 ctcgaccgac ttcaattcgt tgcgcaaagt ggaagatatt cccgccgcgg attcacacgg
      301 attttcgcgg attccttcgc tttcgaagaa ttctggatcg agtttccgca acaccggcac
      361 cagccgctcg atcgtcactt caggccgctt cagaagttgc gccagcgact ggccggcggc
      421 cccgggcaga aggcctgccg agctttgctc ggcctggacg ggcgaggcgc ccgcccccac
      481 atggctgttg ccgatcgccg cggcttgcat cccctccagc atttctggcg tcaggcgggt
      541 gccctccagc agcttcttca tcttctccag ccgcgcctgc ttagcctgaa agtccgccca
      601 cgcctcgttg gagatcagtc ccactcgacg gccgtgcggg gttagccggc ggtcggcgtt
      661 gtcgatgcgc aggtgcagcc ggaattcggc ccgcgaggtg aacatgcgat acggttcgtt
      721 cgtgcccttc gaaataaggt cgtcgatcag gatcgccgtg taagcctcag tgcggtcgag
      781 gatcagaggc ggttcctgct tcacctgcag cgctgcgttg attcccgcca tgattccctg
      841 gcacgccgct tcttcgtagc ccgacgtgcc gttgatctgc ccggcgagga acagtccggc
      901 gatctttcgc gtctccagcg tgcgctccag ttcggtgggg tcgatcgagt cgtactcgat
      961 ggcatagccg ggccgcagca tctcggcggt ttcaaggccg gggatcgact tcaaaatcgc
     1021 cagctgtacg tcgaccggca acgatgtgct catgccgttg acgtaaatct catgcgtgtt
     1081 cttcccttcc ggctcaagga agagctggtg cgtttccttg tcggggaatt tcacgatctt
     1141 gtcttcgatc gacgggcagt accgcggccc gatcgactga atctggccgc tgtacatggg
     1201 cgagcgatgc acgttttggc ggatgatgcg gtgcgtctcg gctgtggtcc acgcgatgta
     1261 gcagggaacc tgggcgtcgt gatgcgcgac ccgccgggtg cggaagctaa agggcgtggg
     1321 atcgtcatcg ccgggctgca ctgggaagcg cgaccattcg atcgagcgtc cgtcgagccg
     1381 cggcggcgtg ccggtcttca atcggcaacc gcgcaggcca agcttcttca gggcctcacc
     1441 gagaaggacg gcgttgggct cgccggaacg tcccgccggg tactgctgct cgccgcagtg
     1501 gatgagcccg ttcaggaacg tccccgtagt gatgatgaca gcctgagccc cgacggtgcg
     1561 gccgtcgcgc agcttgacac cgatgacgcg gcttctggcg tccggccctc gccttccgga
     1621 aaccgctgct gaatgctgct ccctggcttg gccgggcgcc ggaagctggg agccgggagc
     1681 cggttccacg atcaaatccg ctacttccgc ctgcttgatc ttcaggttcg cctgcgactc
     1741 cagcacctcg cgcatcttca ggcggtaggc ctgcttgtcg cactgtgcac gcggcgacca
     1801 caccgccggg ccgcgggaag tgttcagcag ccgaaactgg atgcctaccg cgtcggtaat
     1861 ctcgcccatg atgccgccca gcgcgtccac ttcccgcacc aagtggccct tggcgattcc
     1921 tccaacggcc gggttgcacg acatctgcgc aatcaggtcg acgttgagcg tgtacagagc
     1981 tgtcttcagc ctcatacgtg ccgaggccat ggcagcctca cagccggcat ggccagcacc
     2041 caccacgaca acgtcgaatt gttccgtgaa gtgcatcaag actggtgcat gccccaaatt
     2101 tgttacgttc tttactgctt tcattctagc aacttggagc catttgtgat tcccctcact
     2161 tgccgcaccc ggcgcggccc cgtatattag cctgtcatca cgaaacgacg ggggatttat
     2221 gactaccgct gaatttttcg accaactgga ggcgcgcatc gccaaatatg acctgctctg
     2281 ccaccccttc taccaggcat ggtcggcggg ggagctcagc cgggaagatc ttcgggaata
     2341 cgcgcaggat tactaccacc acgtcgaggc gttcccgtcc tatctggctg ctttcggctt
     2401 gcgactggag gacggcgagc tccgccgagc cgtactcgcg aacctctgcg acgaaaaggg
     2461 aattgaaggc cgggcgggca aggaatcggt tcctcactcc gatctctggc tcgacttcgc
     2521 cgagggcatg ggttccactc gcaatctgga gtggcactgc cccgcgccgg aagtccggca
     2581 gctcatccgc cacttccatc gcgtggccaa cgaaggcctg cccgaagagg ccctggccac
     2641 cttctacgct tatgagtcgc aggtgccgcg catcgcgcgg gagaaggagc gcgggttgcg
     2701 tcagaactat ggcgccgacg acaagacgtg cggctatttc tccctgcatt ccaccgcaga
     2761 tgtgcatcac gcgcgggtgt ggcacacaca gcttgaaaaa cgtatcgcgg ccaaccccga
     2821 agctgccgat gccgcgctgg acgctgccga gaaggccgca cgcgccctgt ggcacgcgct
     2881 ggatggcatc gatgcgcgcc gcatggccat ggcagcatag cggaccgaat cccgggtgtc
     2941 atcctgagcg gagccgttct tccggcggag cgaaggatct ccttgcgaga gaactgcgaa
     3001 gtttgttcct ggggagatcc ctcccgcggc tgaacttcgc cgcagttcgg gattacgcct
     3061 tcggagtgtg acctgcgtcc cacgtaaagt aaatggcccg acccgtcccc atcggcgtgc
     3121 gcggcgagac gcaggaaacc gtcgcctttg agcacacgct gacctcccac aacccgaagc
     3181 tgccgcccgt ctattccact cccgacatga tccggctgat ggagattgca gccttccagg
     3241 cgttgcagcc ttactgtaaa ggcgatgaga tcacggtggg aacatccatc aacgtcgaac
     3301 accgcgccgc cagcggcatc ggagcccata tcacggccga agccatcctc gaatcgtttg
     3361 acggacgctt ctataccctc cgcgtcgccg cccgcgacga cgcgcgcgaa atcggccgcg
     3421 gcaccgtgag ccgggcggtg gtgagcctgg gcaagtttgc cgacagaatg aaacaaaggt
     3481 gataagccgg gatgccccat ttctcgcgcg ttctgtgcgc aagaagtggg gctttgggca
     3541 tgaacgccgg accgctagaa cagcgccgtc gggcgccgct tccagctctt gcccttttcc
     3601 tgagcgatct tctccatgtc ggcgacgtag gctcccgcct gcgtcagcac atggtgcagg
     3661 tcgttgccga tgctgatgaa ggtaaatcct ttttcaagga actcacctac gcgcgccgtc
     3721 ccgaacaaaa acagcccaag aatgacgttg tttttgcgag cgtgctcgat cagcttcttc
     3781 gtcgccgcgt cgagttcagg cgaagtgtac atgtgcggga actcatactt gttatacagg
     3841 cccatcgaca tgcagagatc gttctggcca aggaagagaa tatccacgcc cggcacggcg
     3901 gcgatctcgg caatgttctc aatgcacgag gccgtctcga cctgcagcgc aacgatgccc
     3961 tcgttattcc acgagccggc gtagccaagc agcccggcgc ggttcatgct ccgctgcggg
     4021 aagtaaactg agcgcgtgcc aaccgtcgga tatttcgtgc aactgacggc ctgccgcgcc
     4081 tcgtcggcgg tatttatgta gggaataagg acgccgtccg cgcccatgtc gagcgcctgc
     4141 tgaatgcccg gccgatcgtc gtatccgccg acgcgaacca gcgacttcgc gccgccgttc
     4201 gcaatgccgc tgagcatcgc cgagagtttt tcatagccca tcggcccgtg ctgcgtgtcg
     4261 accagcagcc agtcatagcc actgtgcgcg agttgttcgg caacggtcgg gctatgggcg
     4321 ttgaggaaca ggcccatctt gggctgtcct gcacgcagct gcttcttgaa ttcggatccg
     4381 ctgagagcgg ggtctggcat gaagcctcct tgaagtcgaa tttggtagtg tagcgtatcg
     4441 ggcgatcgcc taacgggaaa cgccgcgtta aatttctcac cgagatgtga cccgcagaca
     4501 gattcaagcg cgaatccctg gtcgggcact gcgtccggcc cgacggaatg acaaggcatc
     4561 caatctgagc ctatagtgga acgcgccagg agatcccgat gcgcatgctt ttgaactggg
     4621 tcttaagtgc ggtcgcggtg tggatcgtcg ctcagcttgt accgggtgtg cacgtcagcg
     4681 ggccagtggc ggcgctgatt gctgcgctgg ccattggatt catcaacgca acccttggca
     4741 tgctgcttaa aatcattacc ttccctctga cactgctgac gctcggcctg ttctggctgg
     4801 tgatcaacgc gctgatgctc aaactggcct cggccctggt gcccggattc caggtgcgcg
     4861 gattcctcgc cgccttcatc ggcgcaatag tactcagcct ggtcaacctg gtgctgaagg
     4921 gaatcgtgat gccgcgcaag gaccagaaat aaacttccca ggtctcaccc ggccggcgca
     4981 tccagccgtg ccgccgcgcg aatcttcttc gacacctgtt caggatcgaa aatcccctgc
     5041 atccacgtgc gataccactg atagcggcgc ccgctgcgcg tgcgcactac aaccgctgac
     5101 ggttcgtcgc tgccgacctt gtcaacttct gaggaaacaa tctgatcgta gggaaccaac
     5161 tgaaattcgt ggccggagtc gtagactaga aatgacgccg tgaagcgaaa gggaccgacg
     5221 acgtcgaccg acccctgcat ctcccggtcg agacgctcag aaacctccgc cagatcgcca
     5281 taccgcagca actcgtggtt cacgggatgc ttactggggt agcgcgccca gatcgcgggg
     5341 aacgccccga agaacagtgc agcagccgcg aaaagcccga accctgcggt gctcccgccg
     5401 cgaaagccgc gcggccagcc aaaccaggcg cagaccagcg cccaacaggc aaacacgaaa
     5461 gacgccagca ggaatgacaa attgatcccg cggattttgc gcctggtgta tgccatctag
     5521 cttttcggcg ccgatcccgg cttcttcggc ttcatcgcct gccattcctc ttccctcagc
     5581 aggcgcccca ggttgctgaa ttctcccgcc ccttgcagga attcgccgcc atccatgcgg
     5641 acgacttcac cgttgatgta ccctgagccg tcactcacca gaaacgccgc gaggttggcc
     5701 acttcctcga ccgtgccaaa gcggtgcagc ggattgcggt tgagcgcctg ttgctccagt
     5761 tcgggacgag gcagcaggcg cgaaaacgct ccctgcgtcg gaatcggccc gggagcgatc
     5821 gcattcatgc gaattccgcg atttccccac tccacggcga ggctgcgtgt gagcgcctcg
     5881 actccggcct tcgaaaccgc cgacggcaca acatacgccg atcccacggg cgcgtacgtc
     5941 gcggagatgc tgagcaccgt gcccgcgcgc tttcccgaga gccagcgccg cccgcacgcc
     6001 gtcgtgcagt tgagcgtgcc catgagcaca atgctgatca ccgaattcca cgcgccagga
     6061 gaaagctcct ccgaccgcgc gatgaaattc ccggcagcgt tattgacgag cacatccagc
     6121 gcaccctctt tccagatgga atccagcatg ccttcgacag cttcaagact gcggacgtcg
     6181 caggggattg catgaatttt gcctttgggc gagagttctg cggcggttgc ttttagaact
     6241 tcttcgcggc ggccgcaaat gtagacgacc gctccaagct cgagaaagcg ttgtgccatg
     6301 cctttgccga ggccggtgcc gccgccggta ataagtatgc gtttgttctg tagaagatca
     6361 gcacggaaca tacgtcctca ctggagacat aggtcttgct gcaagactgg taaattctag
     6421 cccctatgtc atcctgagcg cagcgaagga tctatgcggt ttgccgggcg cgcgccgact
     6481 gcgcaggttc cgcctaggga tgacgacaac tacagatcgc acgccacgcg gaatccgtag
     6541 tcggcatact gaaattccgg aggaatgctt gaccgcgccg agcagcgcgc cactttgatg
     6601 tggtgccgcc acgacccgcc gcgcgaggct ttgcgttgcg gctgcatcgg gcttcgttcc
     6661 ggcccgcgcg gattgcgctc aggcgagacc gcatagtaat tcgggtcgta ccagtcgctg
     6721 caccactcgt gcacgttgtc gcccatgtcg taaagaccga aagtgctcgg agcatactgc
     6781 gcaacgggct caggtccgtt ttgccaccgc gtcgcgtagt ccgggagcga ttggggcggc
     6841 tcgtcacccc agggaaattg cttctgttca agcccgccgc gcaccgcgcg ctcccattcg
     6901 gcttcgctgg gaaggcgata tctgcgtccg gtctgcgaag tcagccattc gcagtacagc
     6961 cttgcttcgt gccaggagac tcccgtcacg ggctgctggg gatggttgaa attcgcatct
     7021 cgccaaaacg gcggctcctg cggggtcgtc gcgcgcagga agcgctcgta ctcggcgttg
     7081 gtcacttgcg tggcggcgag ccgaaaagta tc
//
LOCUS       DASPUL010000546         5533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_935556, whole genome shotgun sequence.
ACCESSION   DASPUL010000546 DASPUL010000000
VERSION     DASPUL010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935556"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VEJ00_12755"
     CDS             complement(<1..273)
                     /locus_tag="VEJ00_12755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02078.1"
                     /translation="MDQVTETLTRNRRRRLARFVALTVALLVPLSAFGGKKKKEVPTP
                     APVIDYSNIVWPNPPAIARIRYQAFYASQQISQVNTAPTKKEKWMDR"
     gene            complement(302..1780)
                     /locus_tag="VEJ00_12760"
     CDS             complement(302..1780)
                     /locus_tag="VEJ00_12760"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXX02079.1"
                     /translation="MKLSPMVPVRLSRFLSPCTARLAFILKLTFFLVIAGLPALAKEH
                     PVPLDKNIEPAKCLECHEDKTKGAVVHSAIAKGCFSCHEVRVSHDITRVKLITSTPQS
                     LCLSCHANKDAAKLAGTVHPPAVRDCLKCHDPHSSDNKNELLKPTVGDKGQNLCLACH
                     TQGLNVPEKGSRHAALDMGCDACHITHKTGQAGKPEFDFHLTKAAPALCLDCHDGKAA
                     DLQKAHHDQPFATADCTTCHDPHQSRVPKLMRQFLHPPFAEKSCDLCHAPAKNGKVVL
                     TQADVKSLCVTCHGDQAEKIEKAKVPHPGAASDCTDCHNPHASRQPGLPKTNAVEICL
                     SCHSDLAEQGKKHYLHQPAFKQACAICHEPHGGENEHLLRAASMDAICLECHGPDSQP
                     RKLESEHVIAIFNGSVKLPEDYFTKNKVPILPLKFGRGHPIAGHPINDVVDPSDVTKL
                     IAKIDCLSCHQPHASAQPGLLVKDQANNAAFCATCHKDLTKR"
     gene            2211..3659
                     /locus_tag="VEJ00_12765"
     CDS             2211..3659
                     /locus_tag="VEJ00_12765"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HXX02080.1"
                     /translation="MALPVRTALRKTWQTLSSIKTGVVLIILVVIFSAAGTVILQRPM
                     TDADDMQHAYSPAMLRFLDATGLTDVFHARWFVLLLVLVSCSIIAASVERFPNAWRFF
                     ARPYKSPDETFRRVLPTQAQIKIGDEEQGLSAAERALHHLHLGSERIVHTNSFSLFSE
                     RNRISEMAVYIVHASLLLIFLGGIVDALYGWRAFMMLTPGSASNQVEMKNGANRTIPF
                     AIRCDAAGEETYKDGTPKRYWSKLAVVDDGREMEKKEIVVNDPLVYQGLRFYQASYGR
                     TGKLDQLILNATPASSAIPTPREVTLTMNQPVALDADTAVELVEFVPDYVVEDGHVYT
                     RSTDMVNPAVHLIVTSRKANTSVNFWMPEIPGVAENASSPYMFEVKDLKTGVFTGLQV
                     SHEPGQWAVWAGVVLMAIGLTFVFYVAHMRFWVVPLRDARGQLVLWVGGTANRNRDAF
                     EQKFKKVVEQIQKEVLADVVDSPASQATIARP"
     gene            3707..5104
                     /gene="ccsB"
                     /locus_tag="VEJ00_12770"
     CDS             3707..5104
                     /gene="ccsB"
                     /locus_tag="VEJ00_12770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HXX02081.1"
                     /translation="MSRARAEQHPATGTTLIVMVGLSIAFLLFMAFLNVVKSGNILNE
                     SNMLYAALIFYAGAGALYLGFGVTAIESYIKFASLATWAGLIANTCAVAHRWYEAGHP
                     PFASVYEMLLSFVWTLAALTLIAEKRYGVKVIGTVTMPVTIVGVVLMQLLRTEVHPLV
                     PALQSTWLHVHVTLAMLAYAACALSFALAMMFLIQDKMKTETFLLATSVFTVGIYAAI
                     LTRFERWGGFNVIAWDPENKAEVFISKGVRLFVTIPDLGWLMLLVFAAVAAPLVLSTL
                     ARVKNHEGFLSIANRAVFISILLQTLALVFFLLRVRDGQYPSLDAEGLYPTSLATSPF
                     ILSALVGGIFANLLYLLLLWRRTDLERLLPDADALDRITYKTICIAFPLLTLMIAAGA
                     YWANRAWGSYWNWDPKETWAAITWLVYAGYLHMRITRGWRGRRAAYFAILGFAVVMFT
                     FFGVTYLLNGLHSYA"
     gene            5097..>5533
                     /locus_tag="VEJ00_12775"
     CDS             5097..>5533
                     /locus_tag="VEJ00_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02082.1"
                     /translation="MPEIIMELTNPEREPETSSESPLQKSSGPRDTGRAASHDRTYAV
                     PVEKDLFLEQAESPEMPWLRINWEIKALLPIALVLLGGMLLFLLATLSWRDPERHTVL
                     LVAGAGAVAICGALLVVLTYTVQRPMVELQQKIAQLGAGDLTVA"
BASE COUNT         1012 a         1549 c         1759 g         1213 t
ORIGIN      
        1 ccgatccatc cacttttcct ttttagtggg agcggtattg acttgcgaaa tttgctgcga
       61 cgcgtaaaac gcctgatagc ggatgcgggc gatggccggc ggattcggcc acacgatgtt
      121 ggagtagtcg ataacagggg ccggcgtagg cacctctttc ttcttcttcc ccccgaaagc
      181 agacagagga accagcagcg ccaccgtcaa tgcgacaaac cgggcaagcc ggcgccggcg
      241 attgcgagtc agagtttccg tgacttgatc catactgttc ctcttcctgg tctgcataac
      301 cctacctctt ggtcagatcc ttgtggcacg tggcgcagaa cgccgcgttg ttggcctggt
      361 ccttcaccag caacccaggc tgagcggaag catgcggctg gtggcaggac aggcagtcga
      421 tcttggcaat cagcttggtg acgtcagatg ggtcgaccac gtcgttgatg gggtggccgg
      481 cgatcgggtg tccgcgtccg aacttcaggg gcagtatcgg caccttgttt ttcgtgaagt
      541 aatcctcggg gagcttgacg ctgccgttaa aaatcgcgat cacatgctcg gattccagct
      601 tcctgggctg ggagtcaggc ccatggcact ccaggcagat ggcgtccatg ctcgccgcgc
      661 gcagcaggtg ctcgttctca ccgccgtgtg gctcgtggca gatggcgcac gcttgtttga
      721 aagccggctg gtggagatag tgcttctttc cctgctcggc cagatcgctg tggcaactga
      781 ggcagatctc gaccgcgttg gtcttgggca ggccgggctg acggctggcg tgaggattat
      841 ggcagtcagt gcaatcgctg gcagcgcccg gatgcggcac tttggccttt tcaatctttt
      901 ccgcctgatc gccgtgacag gtcacgcaca gcgatttcac gtccgcctgc gtcaggacca
      961 ccttgccatt cttggccgga gcgtggcaca ggtcacagga cttctcggcg aacggcgggt
     1021 gcaggaactg ccgcatcagc ttgggaacgc gcgactggtg cggatcgtgg caggtagtgc
     1081 agtcggcggt ggcgaagggc tggtcgtgat gcgccttctg cagatcggcg gccttgccgt
     1141 cgtggcaatc gaggcacaga gccggagcag ccttggtcag atgaaaatca aactccggtt
     1201 ttcccgcttg gccggtcttg tgtgtgatgt ggcaggcgtc gcagcccatg tcgagcgctg
     1261 cgtggcggct gcctttttcc gggacgttca atccctgcgt gtggcaggca aggcagagat
     1321 tctgtccttt atcgcccact gtcggcttca gcaactcatt cttgttgtcg ctcgagtgcg
     1381 gatcgtggca cttgaggcag tcgcgcacgg cgggaggatg cacggttccc gcaagtttgg
     1441 cggcgtcttt gttggcgtgg caactcaggc agagcgattg cggagtcgag gtgatcagct
     1501 tgacgcgagt gatgtcgtgg cttacccgaa cttcgtggca gctgaaacag cctttggcaa
     1561 tcgccgaatg gacgaccgcg cctttcgttt tgtcctcgtg acactcgagg cacttggcag
     1621 gctcgatgtt cttgtcgagc gggacaggat gttccttggc cagggcgggc aatccggcga
     1681 tcaccaggaa aaatgtcaac ttcagaatga atgccagcct tgctgtgcag ggagaaagga
     1741 aacggcttaa acgtactggg accatgggcg acagtttcat ttgcacgatc ctggccttag
     1801 ctgcccggag ccggtgggcg cctgaggccg tcccgtaaat tattgcaaca aacacgtttc
     1861 ttggcgtagg aggcaaggac cgaaattccc agaactcggt tcgagtttcc tgaaatggac
     1921 ctcggatggg ccttctccca catcggctcg gtgaaatcac gcacgacctt aagattgatt
     1981 cgagagcgcg gtccggccca ttttcgcacg atttgtgatt gaaatcacaa cacttccagc
     2041 attgcgcgcg ttcgcggtaa gtccttgggg aagatgggtg ttatggccca ttgcagcccc
     2101 cgcggtcttc cattcgttgg ccgctaatcg attgagcgca aatggtttgt cggaaaaacg
     2161 atgagttgga gcggaacgtg cattgttccc gtggcgtggg gaatcatgac atggcacttc
     2221 ctgttcgcac tgcgttgcgc aaaacctggc agacgttgag ttcgatcaag acgggggtcg
     2281 tgctgatcat tctggtggtc atcttctcgg ctgccgggac agtaattctg cagcggccca
     2341 tgaccgacgc cgacgacatg cagcacgcct attcgccggc catgctgcgt tttctggacg
     2401 caaccggcct gaccgacgtg ttccatgcgc gctggttcgt gctgctgctg gtgctggtga
     2461 gctgcagcat catcgcggcg tcggtggagc gctttccgaa cgcgtggcga tttttcgctc
     2521 gtccctataa aagtccggac gagactttcc gccgggttct gcccacgcag gcgcagatca
     2581 agatcggcga cgaggagcag gggctgagcg cagccgagcg cgccctgcat cacctgcatc
     2641 tcggctctga aagaatcgtg cacaccaaca gcttctcgct tttctcggaa cgcaaccgga
     2701 tctccgagat ggcggtctac atcgtgcacg ccagcctgct gctgatcttc ctgggcggca
     2761 tcgtggatgc cctctacggc tggcgcgcct tcatgatgct tacgccgggc agcgcgagca
     2821 accaggtcga aatgaagaat ggcgcgaacc gcacgatccc gttcgcgatc cgctgcgacg
     2881 cggcgggcga ggagacttac aaagatggca cccccaagcg ctattggtcg aagctggcgg
     2941 tggtcgacga tggccgcgag atggagaaga aagaaatcgt ggtcaacgat ccgctggttt
     3001 atcaggggtt gcgtttctat caagccagct acggacgaac cgggaagctg gatcaactga
     3061 tacttaacgc cactccggcg agtagcgcga tacccactcc gcgagaagtg acgctgacca
     3121 tgaatcaacc ggtcgcgctg gatgccgata cagccgtgga gctggtggaa ttcgtcccgg
     3181 actatgtggt cgaggatggc cacgtctata cgcgcagcac tgacatggtg aatcctgccg
     3241 tgcacctgat tgtcacttcg agaaaggcaa ataccagcgt gaacttctgg atgccggaga
     3301 ttccgggcgt cgccgagaat gcgtcctcac cgtacatgtt tgaagtgaag gatctgaaaa
     3361 cgggcgtgtt caccggactg caagtctcgc acgagcccgg gcagtgggcg gtgtgggcgg
     3421 gtgtggtgct gatggctatc ggattgacct ttgtgttcta cgtggcgcac atgcggttct
     3481 gggtagttcc acttcgcgat gcgcgcggtc agctggtgct gtgggtggga ggcacggcca
     3541 accgcaatcg cgatgctttc gagcagaaat tcaagaaggt agtggaacag attcagaagg
     3601 aagtgctggc ggacgttgtg gattcgccgg cgtcgcaagc aacgatcgcc agaccctgag
     3661 cctggctgtg gggactgact agcatcctga ctgggaggaa atcaaaatgt cacgcgcacg
     3721 agccgaacag catcccgcaa ccggcacgac cctgattgtg atggtcgggc tgagcattgc
     3781 gtttctgctg ttcatggcgt ttttgaacgt ggtgaagtcg gggaacatcc tgaacgagag
     3841 caacatgctc tacgcggcgc tgatctttta cgcgggcgcc ggagcgctct atctggggtt
     3901 tggtgtcacc gctatcgagt cgtacatcaa gtttgcctct ctggccacct gggccggcct
     3961 catcgccaat acctgcgcgg tagcacatcg ctggtatgag gccggccacc ctcccttcgc
     4021 cagcgtctac gaaatgctgc tgagcttcgt gtggacgctg gctgcgctca cgctgatcgc
     4081 ggagaaacgt tacggagtga aggtgatcgg caccgtgacc atgccggtga cgatcgtggg
     4141 cgtggttctc atgcagttgc tgcggaccga ggttcatcca ctggttccgg cgctgcaatc
     4201 cacctggctg catgtgcacg tgacgctggc catgctggct tatgccgcct gcgcgctgag
     4261 ctttgcgctg gccatgatgt tcctgatcca ggacaagatg aagaccgaga ccttcctgct
     4321 agctaccagc gtgtttaccg tgggcatcta tgccgcgatc ctgacgcgct ttgagcgctg
     4381 gggcgggttc aacgtgattg cgtgggatcc ggagaacaag gcggaagtct tcatttccaa
     4441 gggcgtgcgg ttgtttgtga cgattcccga tctcggctgg ctgatgctgc tggtgttcgc
     4501 cgcggtggcg gcgccgctgg tgctcagcac tctcgcgcgc gtgaaaaatc acgaaggctt
     4561 cctgagcatc gcgaatcgcg cagtcttcat cagcatcctg ttgcagacgc tggccctggt
     4621 tttcttcctg ctgcgcgtgc gggatggaca atatccatcg ctcgatgccg aaggcttgta
     4681 tccgacgagc ctggcgacca gcccattcat cctgtcggcg ctggtgggtg gaatcttcgc
     4741 gaacctgctt tatctgctgc tgctgtggcg gcgcaccgat ctggagcggt tgctgcccga
     4801 cgcggacgct ctcgaccgca tcacctacaa gaccatttgc atagctttcc ctctgctcac
     4861 cctgatgatc gccgccggcg cctattgggc gaaccgggcc tggggctctt actggaactg
     4921 ggatcccaaa gagacgtggg cggcgattac ctggctggtg tatgccggct atctgcatat
     4981 gcgcatcacg cgcggctggc ggggtcggcg ggcggcatac ttcgcgattc ttggattcgc
     5041 ggtggtgatg ttcaccttct tcggtgtgac gtatttgctg aacggattgc actcgtatgc
     5101 ctgagatcat catggaactg acgaaccctg agagggaacc tgagacttcg tcggagtcgc
     5161 cgctgcagaa aagcagcggc ccccgcgaca ccggccgcgc ggcgtcacac gaccgcactt
     5221 atgcggtgcc tgtcgaaaag gatcttttcc tggaacaagc ggagagtccc gaaatgccct
     5281 ggctgcgcat caactgggaa atcaaggcgc tgctgccgat cgcgctggta ctgctgggtg
     5341 ggatgctgct gttcctgcta gccacgcttt cctggcgcga cccggagcgg cacacggtgc
     5401 tgctggtagc cggagcaggc gcagtggcga tctgcggagc cctgctggtc gtgctcactt
     5461 acaccgtgca gcggcccatg gtggagttgc agcagaagat cgcccagcta ggagctgggg
     5521 atttgaccgt agc
//
LOCUS       DASPUL010000547         5090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_100466, whole genome shotgun sequence.
ACCESSION   DASPUL010000547 DASPUL010000000
VERSION     DASPUL010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100466"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..2555
                     /locus_tag="VEJ00_12780"
     CDS             105..2555
                     /locus_tag="VEJ00_12780"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HXX02083.1"
                     /translation="MSPRLNPAFMDYLASVFVPTGNRRLNELIGLLLCVSALLLFLAL
                     VSYSPLDPSWNSASALTGSHAARNWIGVVGAYAADMFLQFFGIGAFLLPLFPAILGVR
                     WFRSMKVQAPLAKSLGGIWLMMFIPAMLALLPGHLRWMSVIPMEGLMGRVVGDFLIHY
                     LNLVGAYIVCATVLAVALYLSTAFSFSAIQLWAPTRFAFVTALWSRYRDWQEERLRKR
                     QQKELEQRRISKPVVKTQLIPSRPAQFTEPPASEPRRTGIERMMAEENVGTAAPGCPV
                     ERSSTASSGILAESLPAGPETTSDPEVTARADSGHKAKTTMPRIAGGYKLPSSSLLHR
                     SEERQAVDADELKLLAQVLTEKYAEFDVHGQVTQINPGPVVTTFEFKPEAGIKYSRIT
                     NLCDDLCLALKAESILIERMAGKSTVGIQVPNREREIIWLRENIESQEFMGSKSKLTM
                     AMGKDINGRIVTADLAGMPHLLIAGSTGTGKSVAINAMIMSILYKSTPDQVRLILVDP
                     KRLELGNYEGIPHLYTPIITEPKLAANALRNAVREMERRLKLLASKGVRNIDQYNRLF
                     DQSETPSLFAEESEDQPIPYIVIIIDELADLMMLDSSNVEESITRLAQMARAVGIHLV
                     LATQRPSVDVITGLIKANFPARVSFRVATKVDSRTILDANGAEALLGKGDMLYLPSGS
                     ARVHRLHAPFVTEKEIAAVVEFWKSQGTAEYQQAFLEAPKEAQDPAIGTNDGGPIEEG
                     EAENDPMYGDAVKLVVEFGKASTSLLQRRLRIGYGRAAHLIDLMEQDGIVGAADGPKP
                     REVLKRPDWISEIEESMR"
     gene            complement(2760..2981)
                     /locus_tag="VEJ00_12785"
     CDS             complement(2760..2981)
                     /locus_tag="VEJ00_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02084.1"
                     /translation="MHALIGGDHATASVPGVSVSGNGFALLAGGGVDFNFNRYLAFRM
                     PQADWMLVHGNGSTSGKNLRISFGVVVKF"
     gene            complement(2969..3274)
                     /locus_tag="VEJ00_12790"
     CDS             complement(2969..3274)
                     /locus_tag="VEJ00_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02085.1"
                     /translation="MRKVAMVGCFLALCAISAAAANYPKAELFGGYQFTHLEGGPNAN
                     GWNFAVNGNFSDSFGITADLASAYATESGVNFSNYTSRSVRFYRCGLTSGTRPLCTP"
     gene            complement(3469..4437)
                     /locus_tag="VEJ00_12795"
     CDS             complement(3469..4437)
                     /locus_tag="VEJ00_12795"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXX02086.1"
                     /translation="MPRRYAMIAGSGLRPVPSLVQSTSDVARACAKIGSKIFPEEREP
                     VVLCSKCAASLPEGSHFCLKCGQPVSSPVGSTTSPPTAVLLGCSGCGENLPNGSLFCP
                     KCGKPVSSPAKAAASKSEPVVEVLPPETPPPRRRRKRRVLLWILLALLLGAIAWVISS
                     DNPFAQGLQEIAGWKHDQTILENPFTVSAHSFRYYKFSLPEGSLNVAIVGQFAAAADS
                     RANNRKDKDKDDNAIEVYVLSEPAFTVWQNGYATSSVYQSGRVSDGTVQADLPAGAGI
                     YYLVFNNKFALSTPKSIHATVLLRYKSWLPEWFRRMKDRFWNWLGL"
     gene            4485..>5090
                     /locus_tag="VEJ00_12800"
     CDS             4485..>5090
                     /locus_tag="VEJ00_12800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02087.1"
                     /translation="MEIVFYIGLVLFVLFGPWILLWRVNVRRKNERLEDQLRWADLTG
                     RIHALERELRDLRAGASSAAQPAQEPAAELARAVPPPVPDKPPAAPSTPVQSPAEAWG
                     TRKPEIPFPTQPATAPPAPNRAPPTPAAPPDPRIPAPVPSPNFAAVEIEPSLLDRFRS
                     TLDIEEMLGTNWLNKIGIALVVLGVAFFLQLALKSMGPAGKV"
BASE COUNT         1027 a         1634 c         1466 g          963 t
ORIGIN      
        1 ggcaggaaac cacctgaaca ctaccgccca tttccccgat tgtgggaacc ccaccttctt
       61 gtcataatgt aagggctctg gccgattgcg gcattgagac ggtagtgtct ccgcgcctga
      121 atccggcctt catggattat ctggcaagtg tttttgtgcc cacgggcaac cgccggctca
      181 acgagctgat cgggctgctt ctgtgcgtct cggcgctcct cctctttctt gcgcttgtct
      241 cctactctcc ccttgaccct tcgtggaata gcgcctcggc tctcaccggc tcccacgccg
      301 cccgcaactg gatcggagta gtgggtgcct acgcggccga catgtttctg cagttcttcg
      361 ggatcggggc gttcctgctc cctttgtttc ccgccatcct cggcgtgcgc tggttccgct
      421 cgatgaaagt gcaggcgccg ctggccaaga gcctgggcgg catctggctc atgatgttca
      481 tccccgccat gctggcgttg ctcccgggac atctgcgctg gatgagcgta atccccatgg
      541 aaggcctgat ggggcgcgtg gtgggcgact tcctgattca ctatctgaac ctggtcggcg
      601 cctacatcgt ctgcgccacc gtgcttgccg ttgcgctgta tctctcgacc gctttctcct
      661 tctcggcgat tcaactctgg gctccgacgc ggttcgcctt cgtcacggcc ctgtggagcc
      721 gttaccgcga ctggcaggag gagcgcctcc gcaagcggca gcagaaggaa ctggagcagc
      781 gccgcatctc gaagcccgtg gtgaaaacgc agttgatccc gtcacggccg gcacaattca
      841 ccgagcctcc tgcatccgag ccgcgccgca ccggaatcga gcggatgatg gccgaggaaa
      901 atgtagggac agccgccccc ggctgtccgg tcgagcgcag ctcgactgcc agcagcggaa
      961 tcctggcgga atctttgccg gccggccccg agacgacctc cgatcccgaa gtcaccgccc
     1021 gcgccgacag cggccacaag gccaagacca ccatgccgcg catcgccggc ggatacaagc
     1081 tgccctccag cagcctgctc catcgctccg aggaacggca ggccgtcgac gccgacgaac
     1141 tcaagctgct ggcccaggtt ctcactgaga agtatgccga gttcgacgtc catggccagg
     1201 tcacgcagat caaccccggc ccggtcgtga ccaccttcga gttcaaaccc gaagccggca
     1261 tcaaatacag ccgcatcacc aacctctgcg acgatctttg cctcgcctta aaagccgaaa
     1321 gcatcctgat cgaacgcatg gcgggcaaga gtaccgtcgg cattcaggtg ccgaaccgcg
     1381 agcgtgaaat catctggctg cgcgagaaca tcgagtcgca ggaattcatg ggatcgaagt
     1441 ccaagctcac catggcaatg ggcaaggaca tcaacggccg catcgtcacc gccgacctgg
     1501 cgggaatgcc gcacctgctg atcgccggct cgacgggcac aggcaagagc gtagcgatca
     1561 atgccatgat catgtcgatt ctctacaaat cgacgcccga ccaggtgcgg ttgattctcg
     1621 tcgatcccaa gcggctggaa ctgggtaatt acgaaggcat tccccacctc tacacgccga
     1681 tcatcaccga gccgaagctg gcggccaacg ccctgcgcaa cgccgttcgc gagatggagc
     1741 gcaggctgaa gctcctggct tccaagggcg tgcgcaatat cgaccagtac aaccggctgt
     1801 ttgatcagag cgagacgccc agcctgttcg cggaagaatc cgaagaccag ccaatcccct
     1861 acatcgtcat catcatcgac gagttggccg acctgatgat gctcgactcg tcgaacgtcg
     1921 aggaatcgat cacccgcctg gcacagatgg cgcgcgccgt aggtattcac ctcgtgctgg
     1981 cgacgcagcg gccgtcggtc gacgtgatca cgggattgat caaggccaat ttccccgccc
     2041 gcgtttcgtt ccgcgtggca accaaggtcg actcgcgcac catcctcgac gccaacggcg
     2101 ccgaagccct gctcggcaaa ggagacatgc tctatctgcc ctccggatcg gcgcgcgtgc
     2161 atcgcttgca cgctccgttc gtgacggaaa aagaaattgc tgccgtggtc gagttctgga
     2221 agtcgcaggg cacagcggag tatcagcagg ctttcctgga agcgccgaaa gaggcgcaag
     2281 atcccgcgat tggcacaaac gatggcgggc cgatcgaaga aggggaagcc gaaaacgacc
     2341 ctatgtacgg cgatgcggtc aagttagttg ttgagtttgg caaggcttcc acttccctgc
     2401 tgcagcggcg cctgcgcatc ggctacggac gcgccgcaca cctcatcgat ctgatggagc
     2461 aggacggcat cgtgggcgcc gccgacgggc ccaagccccg cgaagtgctc aaacgccccg
     2521 actggatttc agaaatcgaa gagtcgatgc gctaaaaggc gccagtagaa cggaactcaa
     2581 gatgaagggc ggcagaaatg ccccgccctc ttctccatgc cgactagaag caaaagcaat
     2641 cccatgaatt ggtttgaaag ggcgcgactt ccagccgcac cgctcggccc aagaaagagg
     2701 cgggctttag cccctgaggg aagagattct cagtctcccg gttcattctg agatgccgac
     2761 tagaacttga ccactacacc gaacgagatt cgcaggttct tgccgctggt gctgccgttg
     2821 ccatggacca gcatccagtc agcctgaggc atgcgaaaag ccagatatcg gttgaagttg
     2881 aaatcgactc ctcctccagc cagcagtgca aagccattgc cggagacgct gactcccgga
     2941 accgaggcgg tggcatgatc tcccccgatc agggcgtgca caaagggcgt gtaccgcttg
     3001 tcagcccgca acgataaaac cggaccgaac gtgaggtata gttgctgaaa ttcacgccac
     3061 tctcggttgc gtaggccgat gccagatccg cggtaatccc gaacgaatcg ctgaagtttc
     3121 cattgactgc aaagttccag ccattcgcgt tggggccacc ttcgagatgg gtgaactggt
     3181 agccgccgaa caactcagcc ttgggataat ttgccgccgc cgccgaaatc gcacacagag
     3241 ctagaaagca tcccaccatt gcgactttgc gcacgttact cctcctgtgg atgaccagta
     3301 agtcttctcg cggaaatcgc gctggccgct gtgcgtcgag agccagcacc tatgttctcc
     3361 gccgagacag aatccgtagg attgagttga aattgcctcg tcgcaagtgg gcaagtaacc
     3421 gaggccgcgc gccctggtaa tcctgttatc tatttgatat ggaagcgctt acagcccaag
     3481 ccagttccag aagcgatcct tcattctccg gaaccactcc ggcagccagc tcttgtagcg
     3541 cagaagaacg gtagcgtgga ttgacttcgg cgtgctcagg gcaaacttgt tattgaagac
     3601 gaggtagtaa attccggcgc cggcgggaag gtctgcctgc accgtgccgt ccgacacgcg
     3661 gccgctttgg taaaccgaac tggtggcgta tccgttctgc cagacagtga aggcgggctc
     3721 gctgagcacg taaacctcga tggcgttgtc gtccttgtct ttatccttgc gattgttggc
     3781 gcggctgtcc gctgcggctg cgaactgccc gacaatcgcc acgttcagcg atccctccgg
     3841 caacgagaac ttgtaatagc ggaagctgtg cgccgagaca gtaaacggat tctccaggat
     3901 cgtctgatcg tgcttccatc ccgcgatctc ctggaggccc tgcgcgaagg gattgtcgct
     3961 cgaaatgacc caggcgatgg cgccgagcaa tagcgccagc agaatccaca gcaacacacg
     4021 ccgtttgcgg cgacggcgcg ggggcggcgt ttccggtggc agcacttcca cgaccggctc
     4081 gcttttcgaa gcggccgctt tcgccggaga actgaccggc ttgccgcatt tcgggcaaaa
     4141 tagcgatccg ttcggaaggt tctccccgca cccgctgcat cccagaagaa cggcggtcgg
     4201 gggcgatgtg gtgctgccca ccggtgaact gaccggttgc ccgcacttca gacaaaaatg
     4261 tgaaccttcg ggcagacttg cggcgcattt gctgcacaac acgactggct ctctctcttc
     4321 cggaaaaatt ttcgagccga tcttagcaca cgccctcgcc acgtccgatg tgctctgcac
     4381 caagctcggc accggccgca gtcctgagcc tgcgatcatg gcgtatcggc gaggcatcta
     4441 gtagaatcgc gcgaaccgca gagttccctg gaacctcagc gctgatggag attgttttct
     4501 acatcgggct cgtcttgttc gtgctcttcg gcccgtggat actgctgtgg cgggtcaacg
     4561 ttcgccggaa gaacgaacgc ctggaagatc agttgcggtg ggcggacctt accggacgca
     4621 tccacgccct ggaacgcgag cttcgcgacc ttcgcgccgg agcttcttcc gccgcgcaac
     4681 cagcgcaaga accggcggca gagcttgcca gggcagttcc accaccagtt cctgacaagc
     4741 cgcctgcggc accctcaact ccggtgcaga gcccggcgga agcttggggg acgcgcaagc
     4801 cggagattcc attcccaacc cagcccgcca ccgccccgcc ggcaccgaac cgggcgccac
     4861 caaccccagc tgcgcctccg gatccgcgaa tcccggcgcc agtgccctct cccaacttcg
     4921 cagccgtgga aatcgagccc tcgctcttag accgtttcag gtccaccctc gatatcgaag
     4981 agatgttggg caccaattgg ctcaacaaga tcggaatcgc cctggttgtc ctcggggtcg
     5041 cctttttcct gcaactcgca ctcaagagca tgggtcccgc cggcaaggtg
//
LOCUS       DASPUL010000548         4056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1198559, whole genome shotgun sequence.
ACCESSION   DASPUL010000548 DASPUL010000000
VERSION     DASPUL010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198559"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(372..1514)
                     /locus_tag="VEJ00_12805"
     CDS             complement(372..1514)
                     /locus_tag="VEJ00_12805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXX02088.1"
                     /translation="MNPLREKIEEEIRGGGPIPFSRYMELCLYDPELGYYSRNAAQFG
                     KAGDFYTSSDVHAVFGRLMARQFEEMWRVLGCPACITLTELGPGRGLFAQDVLDWSEK
                     KFPDFFRAMHYVLVESSPSLRRQIEKTLAQRLEAWKASLAIESVIADAKDTPAIVFAN
                     EFFDALPVEIVSARGSLRVDTRDGKFVETWAPSSPEELEFLDRYSVHPEAGERLEVAL
                     AAQHYAYQAFANLGRGFFVAIDYGYTRDEQLAGRHRGTLKAIRQHSVSANPYEAPGEQ
                     DITADVNFTALAAAAESQGMHVQGLVTQSQFLMGIGEANQFADAFEDCRLPQERAKVA
                     LQLKHLVTPAGMGEKFHVLVAHKGIETQKAAVLAGLDFGRTMAGRK"
     gene            complement(1511..1813)
                     /locus_tag="VEJ00_12810"
     CDS             complement(1511..1813)
                     /locus_tag="VEJ00_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02089.1"
                     /translation="MSDASSRRLDRVLAQLRLYEHPLLNFQAHTKGEGVEVIIKFRDN
                     SIPVHTYTFELHPRDLDDSQFEWSFQRQLYDALHDYFVEMFIRTPQDRTDRQRRGL"
     gene            complement(1857..3932)
                     /locus_tag="VEJ00_12815"
     CDS             complement(1857..3932)
                     /locus_tag="VEJ00_12815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HXX02090.1"
                     /translation="MNKHARAVLLIGVVTLLFCLAMLAQSTSSAAAEETQQGLPSGLD
                     KRFIDESADPCVNFFQYACGNFPKYYPIPNDRSGFGTGTILFEHNQVVLHAMLDKAAA
                     GGAERTANQQKIGDYYGACMDIDIIDQKGLRAFQPELDRIAALKEKSELTPLLAHYQL
                     INVNAFLGLGEQQDFHDASKQIAITDQGGLGLPERDYYFRTGDAAETTRKQYVQHIKN
                     TLNLIGEPESDATADAQSIFQLETAMAKISMDITSRREPKNIYHPTAVSDLQKATPSL
                     DWTRLLSEARFPSLNELNVTNPDYFKGLNTLLETTDMKTIKAYLRWQLMASADQTALP
                     KAMDEERFDFYGRKLRGQPEQQARWKRCVQATDRALGEALGQVYAAQEFPPSSKAATL
                     QMVRDIENAMDQDLNTLDWMSPETKTRAKAKLHAVADKIGYPDHWRDYSRLAVKRDDA
                     YGNAERATIFENLRQIDKIGKPVDRGEWGMTPPTVNAYYNPSMNDINFPAGILQPPLY
                     DPKASDAENYGHIGGIVGHELTHGFDDQGRQFDGKGNLADWWSPDDEKKFVEKADCTV
                     REYGSFVAVDDVHVNGKLTLGENTADNGGLRLAFLAFLADAKRKNIDLTQKQDGYTPI
                     QQFFLGHGQSWCGSTRPEQQRLQVQTDPHSPREFRVNGVVQNMPEFGQAFGCKVGQPM
                     TPQNSCRVW"
BASE COUNT          789 a         1164 c         1206 g          897 t
ORIGIN      
        1 tcgcgaaatt aaactcaact gcaggcgtcg gtgccgtcaa tgactgatcg tcattgaaga
       61 gaatgccgaa ctcgggccaa aagggttggc ccagctggtt cgcccatcac gagggacaac
      121 gctgccaatc cagacaaaac ggatccgtgc aagagtcgag gctgaagggg cgtcataggt
      181 ttgttctccc gcgggaatcc ccactgaaaa gtctcgcgga aattatcacg gcaaagtccg
      241 atattgcaat agttactaac attttttaca cactcttgac atttagggtg aatgcctgag
      301 atggtcacgc aacagctgtt ctcgcacgcg gggcagtctg ctgcgtcttc agttcgagcg
      361 aatcggggtt gctactttct accggccatc gtgcggccga agtcgaggcc ggcaagaaca
      421 gccgcttttt gtgtctcgat tcctttgtgc gccaccagca catgaaactt ctcgcccatg
      481 ccggcggggg tgaccagatg cttgagttgc agcgcaactt tggcgcgctc ctggggcaag
      541 cggcagtctt cgaaggcatc ggcaaactgg ttagcttcac cgatgcccat cagaaactgc
      601 gactgcgtaa ccagcccttg aacgtgcatc ccctggctct cggctgctgc ggccagggca
      661 gtgaagttca cgtccgccgt gatgtcctgc tcgccgggag cctcatacgg attcgcgctc
      721 accgaatgct gccgaatggc cttcagcgtt ccgcgatgcc ggcctgccag ttgctcgtcg
      781 cgcgtgtagc cgtaatcaat ggcgacaaag aatccgcggc ccagattggc gaaagcctga
      841 tacgcgtagt gttgcgccgc gagcgcaact tccaaacgct ctcccgcctc gggatgaaca
      901 ctgtagcgat cgaggaattc gagttcctcg ggcgaggatg gagcccaggt ctcgacgaac
      961 ttgccgtccc gcgtatctac tcgcaacgag ccccgggcgc tcacaatctc caccggcagg
     1021 gcgtcgaaga attcgttggc gaagacgatt gccggagtgt ccttggcgtc cgcgataaca
     1081 gattcgattg ccaacgatgc cttccacgcc tcaaggcgtt gcgccagcgt tttttcgatc
     1141 tgccgccgca gcgaaggcga ggactccacc aggacgtaat gcatcgcccg aaagaaatcg
     1201 gggaacttct tttccgacca gtccagcaca tcttgcgcga acaagccacg cccagggcca
     1261 agctccgtca gagtgataca tgcggggcag cccaacacgc gccacatctc ctcgaactga
     1321 cgcgccatca atcggccgaa gacggcgtgg acgtcgctcg aagtataaaa atcgcccgct
     1381 ttcccgaatt gtgccgcatt ccgcgagtag tagcccagtt cggggtcgta gaggcacaac
     1441 tccatatagc gcgagaaagg gatgggtccc ccaccgcgga tttcctcttc gatcttttcg
     1501 cggagtgggt tcacagtccc ctgcgctggc ggtcggtgcg atcctgcggc gtgcgaatga
     1561 acatctccac gaaatagtcg tgcagcgcgt cgtagagctg gcgctgaaag ctccattcaa
     1621 actgggaatc gtcgagatcg cgcgggtgca actcgaaggt gtaggtgtgg acgggaatgc
     1681 tgttgtcccg gaacttgatg atgacctcaa cgccctcgcc cttggtgtgg gcttggaaat
     1741 tcagcagcgg gtgttcatac agacgcagct gggcgagcac ccggtcaagg cgacggctgg
     1801 aggcatcgga cacggaagca ttgtacagag cgggaatgct tccgtgccga caggagctac
     1861 cagacgcggc aggagttctg aggcgtcatc ggctgcccga ccttgcagcc gaaagcctgg
     1921 ccgaactcgg gcatgttctg gaccacgcca ttcactcgga attcacgcgg ggaatggggg
     1981 tcggtctgta cctgcagtct ttgctgttca ggtcgcgtgc tgccgcacca gctttggcca
     2041 tggccgagga agaactgctg gatcggtgtg taaccgtcct gtttctgcgt gagatcgatg
     2101 ttcttgcgct tcgcatcggc cagaaatgcg aggaaggcca ggcgcagtcc gccgttatcg
     2161 gcggtattct caccgagagt cagcttgccg ttgacgtgca cgtcgtcgac cgcgacgaag
     2221 ctgccgtatt ccctcacggt gcagtcggcc ttttccacga atttcttttc gtcgtctgga
     2281 ctccaccaat cggcgagatt gccttttcca tcaaactgac gcccctgatc gtcgaagccg
     2341 tgcgtcagtt catggccgac gatcccgcca atgtggccat agttctcggc atcgctggcc
     2401 ttgggatcgt agaggggtgg ctgcaggatg cctgccggaa agttgatgtc attcatgctg
     2461 gggttgtagt aagcgttgac cgtaggcggc gtcatgcccc attcgccgcg gtcgaccggc
     2521 ttgccgatct tgtcgatctg ccgcaggttc tcgaaaatcg tcgcgcgctc tgcattgccg
     2581 taggcgtcgt cacgtttaac agccagcctg gagtagtcgc gccaatggtc gggatagccg
     2641 atcttgtccg ccacggcgtg aagctttgct ttcgcgcgcg tcttcgtctc agggctcatc
     2701 cagtccagcg tgttgagatc ctgatccatc gcattctcga tgtcgcgcac catctgcagc
     2761 gtcgcagcct tgctcgaggg agggaactcc tgcgcagcat acacttgacc caacgcctcg
     2821 cccagtgcgc ggtccgtggc ctgtacgcag cgcttccagc gcgcctgctg ttcaggctgc
     2881 ccccgcagtt ttcgcccgta aaagtcgaac ctttcctcgt ccatcgcttt gggcagagcg
     2941 gtctggtcgg ccgatgccat cagttgccag cgcaggtagg ccttgatcgt cttcatgtca
     3001 gttgtctcga gcagcgtatt cagtcctttg aagtaatcag gattcgtcac gttgagctcg
     3061 ttcaggctgg ggaagcgagc ttccgagagc aggcgcgtcc aatcgaggct gggggtagct
     3121 ttctgcaggt cagatacggc cgtcggatga tagatgtttt tgggctcgcg gcgggaggtg
     3181 atatccattg agatcttcgc catcgccgtt tccagttgaa aaatgctttg ggcatcggcc
     3241 gtcgcatcgc tttcgggctc gccgatcagg ttcagagtat tcttgatgtg ctgcacatat
     3301 tgtttccgag tggtctccgc agcgtcgccg gtgcggaaat agtagtcccg ctccggcaat
     3361 cccagcccgc cctgatcggt gattgcaatc tgtttgctcg cgtcatgaaa gtcctgctgc
     3421 tcgcccaggc cgaggaaggc attgacgttg atcagttggt agtgggcaag cagcggcgtg
     3481 agctcgcttt tctccttaag ggcagcaatg cgatcgagtt ccggttgaaa cgctcggaga
     3541 cccttttgat caattatgtc gatatccatg cacgcgccgt agtagtcgcc gattttctgc
     3601 tgattggcgg tgcgctcggc gccgcccgca gcggccttgt cgagcatggc atgcaaaacc
     3661 acctggttat gttcgaagag tatggtcccc gtgccgaagc cggagcgatc gttaggaatg
     3721 gggtagtact tgggaaagtt tccacaggcg tactggaaga aattcacaca aggatcggcg
     3781 gattcgtcga tgaagcgttt gtccagcccc gacggaagac cctgctgcgt ctcctctgca
     3841 gcagcggaac tggtcgactg cgctaacatc gctaagcaga acaaaagcgt tacaacgccg
     3901 attagcagaa cagcgcgcgc gtgtttattc atggtttcct ctgagcggag ttttttgatc
     3961 cgatggcctc acagcaccgt tgggcagcgt cgtccagcaa gggaagactg cagtgagcgg
     4021 gaagcctttc acggcacatg tgcatttcaa gggtag
//
LOCUS       DASPUL010000549         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1328234, whole genome shotgun sequence.
ACCESSION   DASPUL010000549 DASPUL010000000
VERSION     DASPUL010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328234"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="VEJ00_12820"
     CDS             <1..1396
                     /locus_tag="VEJ00_12820"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXX02091.1"
                     /translation="FLISMEKSINDNKAEAPFLSFKGAQRETGEVLIEGAGTMEITAT
                     EGGGLKRMDVKEASPYLRSLAHFPPQAAFRYHKQADETPTLALAWVRFPDSSVLAAVA
                     ENAVVKTLVTSEGKSLTEVKLTLKNQAQPFLKVALPQGATILSAEVGGEPVKPVQGPD
                     GSRVPLLRPRFHPTDSYTVSFVFMHSGAPFAKKGGAELSLPNMDIPISLLNWEVFLPE
                     QYKVKDFGGDVIAADRVPPAFSEEAAMREYRMREAEVSSNLSNNVRLESDPGQMGGYV
                     VDPTGAVVPNARVTITSPDNGRARTITTDGQGHWVAAAMPSGNFKARVEMAGFRTSIF
                     NLNYDASQPSAYNFPISVASTSEVVEVTAQAAQIQTQTAAIGGTIGGSIVTKSGTNNL
                     PLVGRNMTDLVATSPGTAPQNGASANVVNLQKKVAGVLPVRIDVPRAGTSFSFVRPLV
                     LDEETKVTFSYRSR"
     gene            1513..>2029
                     /locus_tag="VEJ00_12825"
     CDS             1513..>2029
                     /locus_tag="VEJ00_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02092.1"
                     /translation="MSSSVETWTSWVGSVAEGKFPLKQWLGGSDHSAVFLTERQSRQA
                     AIKLVPAVGENAEHHLARWRAASQLSHPHLLRVFESGRCQIDGTALVYVVLEYADEDL
                     GQIFPQRALSSAEVADLLPPLLDALTYLDGKGLVHSRIKPSNILAVHDQLKLSADQIL
                     SATDTNSIRRRD"
BASE COUNT          415 a          589 c          666 g          359 t
ORIGIN      
        1 gttcctgatc tcgatggaga aatcgatcaa cgacaacaaa gccgaagcgc cgtttctcag
       61 cttcaaagga gcgcaacgcg aaaccggcga ggttctgatt gagggcgcgg gtaccatgga
      121 gattacggcc accgaaggcg gcgggctcaa gcgcatggac gtgaaggaag cgagcccgta
      181 tctgcgatcg ctggcgcact tcccgccgca ggcggcgttt cggtaccaca agcaggctga
      241 tgagactccg accctggcgc ttgcgtgggt gcgctttccc gacagcagcg tgcttgcggc
      301 ggtggctgag aacgccgtcg taaagacgct ggtcacttcg gaaggcaaat cgctcaccga
      361 agtgaagctc actctcaaga accaggcaca gccgtttctg aaagtggcac tgccgcaagg
      421 cgcgactatt ctctcggcag aagttggcgg tgaaccggtg aaacctgtgc aaggaccgga
      481 cgggagccgg gttccgctcc tgcggccgag attccatccc accgattcct acacggtctc
      541 gttcgtgttc atgcactccg gcgcaccgtt tgcgaaaaaa ggcggggccg aactcagtct
      601 tccaaatatg gacatcccca tcagtttgct gaactgggag gtgttcctgc cggagcaata
      661 caaagtgaaa gatttcggcg gagacgtgat cgcggccgac cgcgtgccgc cggctttcag
      721 cgaagaggcg gcgatgcgcg agtacaggat gcgggaggct gaagtctcca gcaacttgtc
      781 gaacaatgtg aggctggaat cggatcccgg gcagatgggc ggctacgtcg tggatccgac
      841 cggagccgtt gtgcccaacg cgcgcgtcac gattacctcg cctgacaacg gccgcgcacg
      901 aacgataacg accgacggcc aggggcactg ggtggcggcg gcgatgccct cggggaactt
      961 caaagccaga gtggagatgg cgggattcag gaccagcatc ttcaatctga actacgatgc
     1021 cagccagccc tcggcgtaca actttccgat cagcgtggcc agcacttcgg aggtcgtaga
     1081 ggtgaccgcg caggcagctc agattcaaac gcagactgcc gcgatcggcg gcacgattgg
     1141 cggttcgatc gtcaccaaga gcgggaccaa caaccttccg ctggtggggc gcaatatgac
     1201 ggacctggtc gccacgtcgc ccggaacggc gccacagaat ggcgcttccg ccaacgtggt
     1261 gaacctgcag aaaaaggttg ccggcgtgct gccggtgcgg attgacgtac cgcgggcggg
     1321 gacgtcgttc agcttcgtgc ggcccctcgt gctcgatgag gagaccaagg tgacgttcag
     1381 ctatcggagc cggtagttgg ggtcaagagt cgtggccggg cgggcgcctc gtccgccccc
     1441 tgacgagggc gtaggggctc cacggcccgg ggccggactt ggccgatagc atagaatggt
     1501 cccggcgggc tcatgagcag cagcgtcgag acctggacaa gctgggtagg cagcgtagcc
     1561 gaggggaaat ttccgctcaa gcagtggctg ggcggctcgg atcacagcgc agtctttctc
     1621 accgagcggc aatcccggca agccgcaatc aaactggttc cggcggttgg cgagaacgcc
     1681 gagcatcatc tggcgcgctg gcgcgcggcc tcgcaactgt ctcatcctca cctgctgcgc
     1741 gtctttgaat ccggacgatg ccagatcgac ggaacggcac tggtctatgt ggtgttggag
     1801 tacgctgatg aagacctggg gcagatcttt ccgcagaggg cgttgagttc cgcagaagtt
     1861 gccgacctgc tgccgccact gctggatgca cttacttatt tggatggaaa agggttggtg
     1921 cacagccgca tcaagccctc gaatattctg gcggtgcacg atcagttgaa gctttccgcc
     1981 gatcagattc tctccgccac agacacgaac tcgatccgga ggcgggacg
//
LOCUS       DASPUL010000550         3907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1471293, whole genome shotgun sequence.
ACCESSION   DASPUL010000550 DASPUL010000000
VERSION     DASPUL010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471293"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VEJ00_12830"
     CDS             <1..240
                     /locus_tag="VEJ00_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02093.1"
                     /translation="AIALELDPFVQHGHVIEITGKTQTGARVMVNGSEVPLVTGDGSF
                     HYFTPPLPTGEAVITVTAQTAQGGVNTQQKKIIIQ"
     gene            512..1573
                     /locus_tag="VEJ00_12835"
     CDS             512..1573
                     /locus_tag="VEJ00_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXX02094.1"
                     /translation="MSTNGYSNGTRAHNLRSLFSIFSSDLAIDLGTANTLVYARGKGI
                     VVNEPSIVAINKNTGEVEAVGKEAKEMLGRTPGNIVAIKPMKDGVIADFKVTEKMLNY
                     FIQKAHNRKMLVHPRIVIGVPSEITQVEKRAVMDSAYRAKASEVHLVEQAMVAAIGAG
                     LPITEPSGNMVVDIGGGTTDIAVISLSGIVYSRSVRMAGNQMDEAIMNYLKRKYNLLI
                     GERTAEQIKMEIGSAHQLDKPMTMEIKGRNLIEGVPKTITVDDGEIREALSECVSTIM
                     NAIRVALERTPPELSADISDRGIVLTGGGALLKNLDKRIREETGLPVSIADDPLCSVV
                     LGTGKMLSDFKLLRKISIE"
     gene            1654..2937
                     /gene="mreC"
                     /locus_tag="VEJ00_12840"
     CDS             1654..2937
                     /gene="mreC"
                     /locus_tag="VEJ00_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HXX02095.1"
                     /translation="MESVLGRYRNLIILVGVLFLQVLGLAVQVKRGAGSDEKDTRLIR
                     IWAVGAITPFERVLIWAENSTGNLWHNYFYLRGVRAENRALKDQIERMRLEQVRLSED
                     AAQAHRLQTLLAFKEQYIAKTVAAQVIGTSGSDLSRIVYLDKGENSGLRRDMAVITAD
                     GIVGKVVEVFPSVSKVLLINDQSSGVGVILEKSRLQGVLRGTSNGEVVLERVMSDEQV
                     PVGENVLTSGGDQIFPKGLPVGVITKVGTGKDLFLNIKVKPSTDLSKLEEVLVVAEKQ
                     QRQAVAEETGHVRAADILAQRLPSVPDKPEDAKPADAKPGDAVTTKPASAAPKPQPAE
                     GSNGAGSHAANTATGGAASAAGTAEHKPPTATAVKPLASASGQAQPKNVNLAPDAAKA
                     AIVPAASDSATPPVKTTKPSNPQPEPQAPPDDTPH"
     gene            2921..3460
                     /gene="mreD"
                     /locus_tag="VEJ00_12845"
     CDS             2921..3460
                     /gene="mreD"
                     /locus_tag="VEJ00_12845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HXX02096.1"
                     /translation="MTLHTSVPITYTSGEQVEVYRFSIPVTIGVPLAALFIQAFVPLR
                     FPRFAVYLDLPLLVTIFFAMARRNPISGLFTGAAIGLAQDMLGHNPIGIYGIAKTVVG
                     YGASSLGVKLDVENAGARFLVTLGFYLVHWAVYFTVARGMVSMTQPWSWLHGILAGLA
                     NATLGTFLYFALDRFKQRT"
     gene            3659..>3907
                     /locus_tag="VEJ00_12850"
     CDS             3659..>3907
                     /locus_tag="VEJ00_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02097.1"
                     /translation="MFGRDEKVSEIRLTATQYIVLGIFLLLAYGLWNLQVMKSDYYSL
                     AAERNRIRNVPVLAPRGRILDREGRTIVDNYPSFSALLL"
BASE COUNT          816 a         1111 c         1295 g          685 t
ORIGIN      
        1 gcgattgcgt tggagctgga tccgttcgtg cagcacgggc atgtgatcga aataaccggc
       61 aagacgcaga ccggcgcgcg ggtgatggtg aacgggagcg aggtgccgtt ggtgaccggg
      121 gatggctcgt ttcactattt cacgcccccg ctgcctaccg gcgaggcggt gatcacggtg
      181 acggcgcaga cggcgcaggg cggagtgaat acgcagcaga agaagatcat tattcagtag
      241 gggcacgttt cagggttaag gttaggggtg cttggcgttc tttgcgcatt ctcttcgcga
      301 tctttgcatt gaagctttta ccgcaaagtc cgctaaggag atccgcaaag aacgcgaagc
      361 aatcctgggg cgggccgatc cgcttccggg ttttgagctg gttgccgggt atcccgtaga
      421 atggggatat ccagacatca gaggaaagta acgaagggcc cgggcgaccg ggtgtgacgg
      481 aagtctggaa ccaagcaggg aagatttcgc catgtcaacc aacgggtatt cgaacggaac
      541 gcgcgcgcac aatcttcggt cgctgttcag cattttttcg agcgatctgg cgatcgatct
      601 gggcacggcc aacacgctgg tatacgcgcg gggcaaaggc atcgtggtga acgagccgtc
      661 gatcgtcgcc atcaacaaaa ataccggcga ggtcgaggcc gtaggcaaag aggcgaaaga
      721 gatgctggga cgcacgcccg gcaacatcgt cgccatcaag cccatgaaag acggggtcat
      781 cgccgacttc aaggtcaccg agaagatgct caactacttc atccagaagg cgcacaaccg
      841 caagatgctg gtgcatccgc ggatcgtgat cggcgtgcct tcggagatca cacaggtgga
      901 gaagcgcgcg gtcatggact cggcctatcg cgccaaggcg agcgaagtgc atctggtgga
      961 gcaggccatg gtggcggcga tcggcgcggg gctgcccatt accgaaccca gcggcaacat
     1021 ggtggtcgac atcggcggcg gcaccaccga cattgcggtg atttcactca gtggcatcgt
     1081 ctattcccgc tcggtgcgca tggccggcaa ccagatggac gaggccatca tgaattacct
     1141 gaagcggaaa tataacctgc tgatcgggga gcgcacggcg gagcagatca agatggagat
     1201 cgggtcggcg caccagctcg acaagccgat gacgatggag atcaagggcc gcaacctgat
     1261 cgaaggcgtg cccaagacaa tcacggtcga tgatggcgaa atccgagaag cgctgagcga
     1321 atgcgtgtcg acgatcatga atgccattcg cgtggcgctg gagcggacgc ctccggagct
     1381 ttcggccgac atcagcgacc gcggcatcgt gctgaccggc ggcggggcgc tgctcaagaa
     1441 tctggacaaa cgaatccgcg aagaaaccgg gctgccggtt tcgatcgccg acgatccgct
     1501 gtgcagcgtg gtgctgggca cgggcaagat gctctccgac ttcaagctct tgcgcaaaat
     1561 ttcgatcgag taggaaacaa ggggtcaggg gccagggatc aggggccaga tgaacttact
     1621 gacccctggc ccctgatccc tgacccctga tttatggaat ccgtcctcgg ccgctaccgc
     1681 aatctgatca tcctggtggg cgtgctgttt ctgcaggtgc tgggcctggc ggtgcaggtg
     1741 aaacgcggcg ccggcagcga cgaaaaggac acgcggctga tccgcatctg ggcggtgggc
     1801 gccatcactc ccttcgaacg agtcctgatc tgggccgaaa acagcacggg caatctctgg
     1861 cacaactatt tttatctgcg cggggtgcgc gccgagaacc gcgcgctcaa agaccagatc
     1921 gagcggatgc ggctggagca ggtgcggctg agcgaagacg ccgcgcaggc gcatcggctg
     1981 caaaccctgc tggccttcaa ggaacagtac atcgcgaaga cagttgcggc gcaggtgatc
     2041 gggaccagcg gcagcgatct gtcgcgcatc gtctatctcg acaaaggcga aaacagcggc
     2101 ctgcggcgcg acatggccgt gatcacggcc gacggaattg tgggcaaggt ggtggaagtg
     2161 tttccttcgg tttcgaaggt tctgctgatc aatgatcaat cgagtggcgt gggagtgatc
     2221 ctcgaaaaat cccggctgca gggggttctg cgcggaacct cgaacggcga agtcgtgctg
     2281 gagcgggtga tgagcgacga gcaggtcccg gtcggcgaaa acgtgctgac cagcggcggt
     2341 gatcagatct tcccgaaagg cctgccggtg ggagtgatca cgaaagtggg cacgggcaag
     2401 gacctgttcc tgaacatcaa ggtcaagccc tcgaccgacc tgagcaagct ggaagaagtg
     2461 ctggtggtgg ccgagaaaca gcagcggcag gccgtggccg aggagacggg gcacgtgcgg
     2521 gcggccgaca tcctggcaca gcggctgcct tcggtgccgg acaagcccga ggatgcgaag
     2581 cccgccgacg ctaaacccgg ggacgctgtg accacaaagc cggcaagtgc tgcccccaaa
     2641 cctcaaccag cagaaggatc gaatggagcg ggttcccacg cagccaacac tgcgacgggt
     2701 ggggcagcct cggcagcggg aaccgccgag cataaaccgc cgaccgcgac tgcggtcaaa
     2761 ccgctggctt cggcaagcgg acaggcgcag ccgaagaatg taaatctcgc tccggacgcg
     2821 gcgaaggccg cgattgttcc ggcggcgtct gacagcgcaa cgccgccagt gaagacaacc
     2881 aaaccctcga accctcagcc cgaaccgcag gcaccccccg atgacactcc acactagcgt
     2941 gcccatcacc tacacatccg gggagcaggt tgaggtttac cggttcagca ttccggtgac
     3001 catcggcgtg ccgctggcgg cgctgtttat ccaggcattc gtgccgttac ggtttccgcg
     3061 ctttgccgtt tatctggatc tgccgctgct ggtcacgatt ttctttgcca tggcgcgacg
     3121 gaacccgatt tccggcctct tcaccggagc ggcgatcggc ctggcgcagg acatgctggg
     3181 gcacaatccc attggcatct atgggattgc gaagacggtg gtgggttatg gggcttcgtc
     3241 gctgggcgtg aagctggacg tggagaacgc cggggcgcgt tttctggtga cgctggggtt
     3301 ctacctggtg cactgggccg tctatttcac cgtcgctcgc ggcatggtga gcatgactca
     3361 gccctggagt tggctgcacg ggatcctggc cggactggcc aacgcgacgc tggggacgtt
     3421 tctgtacttc gcgctggacc ggttcaagca gagaacgtga accacggcgt cgggctctcg
     3481 gcgtcgggct tccgcaaaaa tcgagaccgc tgtcccatga ccttcgttac tggaatggcc
     3541 tccgctgcgc aaagggccga tttacaatga ggttaactcc tgagaggacc atggcatcag
     3601 cctcgtctga acgtgtgccg tgcacgccgg aagccggaag cctgaagccc aaagcctgat
     3661 gttcggccgc gacgaaaaag tctccgagat tcgcctgacg gcgacccagt acatcgttct
     3721 ggggatcttc ctgctgctgg catacgggct gtggaacctg caggtgatga agagcgacta
     3781 ctactcgctc gccgcggaaa ggaaccgcat ccgcaacgtg ccggtgctgg cgccgcgggg
     3841 caggattctc gaccgcgaag ggcggacgat cgtcgacaac tatccatcgt tctcggcgct
     3901 gctgctg
//
LOCUS       DASPUL010000551         3974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_101782, whole genome shotgun sequence.
ACCESSION   DASPUL010000551 DASPUL010000000
VERSION     DASPUL010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101782"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..508)
                     /locus_tag="VEJ00_12855"
     CDS             complement(200..508)
                     /locus_tag="VEJ00_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02098.1"
                     /translation="MDAGPVAGKPSYVILYGEGCYNSKRQARRTVSLYGKYKGPVQFV
                     VIDLDHALSPAQQQLQKKYYRGYIPHVVVLDANGAAVYNSSGEVDEGVISSLLDKALR
                     "
     gene            complement(667..1125)
                     /locus_tag="VEJ00_12860"
     CDS             complement(667..1125)
                     /locus_tag="VEJ00_12860"
                     /inference="COORDINATES: protein motif:HMM:NF019238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HXX02099.1"
                     /translation="MKTLRSLRDKQEVLQRVQAIRPNSERRWGRMTPHQMLCHLSDSY
                     RMFMGVRPVKPEAVPYPRSLMKFVAIWAPIPWPHGFKAAPELDQEKGGTPPLQFEADR
                     RDLCNLIDRFTRRPIDFEYQPHPHFGQMSETAWMRLGYLHADHHLRQFGA"
     gene            complement(1241..2734)
                     /locus_tag="VEJ00_12865"
     CDS             complement(1241..2734)
                     /locus_tag="VEJ00_12865"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HXX02100.1"
                     /translation="MLIPYFVVLILLAGYGAHRYILVYLYYKHSKNRTTEPTARFEDL
                     PRVTVQLPIFNEQYVVERLLDAICRLDYPREKLDIQVLDDSTDETVEVARGLVERYAA
                     LGQPISYHHRTNREGFKAGALAEGMKSAEGEFIAIFDADFTPPEDFLLRTIHHFTDPK
                     IGMVQTRWTHLNRGYSFLTQVEAILLDGHFVLEHSGRARTGLFFNFNGTAGMWRRKTI
                     EEAGGWQHDTLTEDTDLSYRAQLKGWKFIYLQDVECPAELPVEMTAFKTQQARWAKGL
                     IQTGKKILPRVLRSDAPLHTKIEAWYHLTANISYPLMIMLSVLLLPAMIIRFYQGWFQ
                     MLYIDLPLFMASTFSISSFYLVSQRELFPRSWPRALLYLPFLMALGIGLTITNTKAVL
                     EALVGKKSAFARTPKYRVITKQDKIGATKYRKRLGWVPWVELLIGGYFALTVYYAVDN
                     ENYITVPFLLLFVVGYWYTGLMSLLQGRFGGRALTATSHTKPFPVGV"
     gene            3131..3490
                     /locus_tag="VEJ00_12870"
     CDS             3131..3490
                     /locus_tag="VEJ00_12870"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXX02101.1"
                     /translation="MPRAATTSDAFNAVAEPRRRQILDFLATQERPVGDIVAGLRLEQ
                     PSVSKHLGVLRSVGLVRVRRNGRHMLYRTNAEAIRPLHEWTKTFERLWAHQLTRIKER
                     AESKTAEQNRSHEKEEA"
     gene            3487..3945
                     /locus_tag="VEJ00_12875"
     CDS             3487..3945
                     /locus_tag="VEJ00_12875"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXX02102.1"
                     /translation="MSAIVTPPGIESLTLVITREIHVKATLENTFAAILEQIGPYNET
                     PEGPMPMKIEPWPGGRWYRDLGDNNGHFWGHVQAIKRPTLLEITGPLFMSYAVVSNLQ
                     YRLSEADGGTLIKFHHKAFGVIQDEHRRGVVKGWDHILGSIRDRAQASTK"
BASE COUNT          827 a         1136 c         1253 g          758 t
ORIGIN      
        1 cctaaggggt ccatggggcc ggcagtcaaa gccgctcctt ctaactcgca actgtcattc
       61 cgagctcagc gaggaatccc tgtcacaggt aatccactac tgggtaggga tgcttcgact
      121 gcgaacgagt tcgctgcgcg atctcgttct tcgctcagca tgacacgaac tttggcgcag
      181 cattcgtgga aaccacggat caccgcaaag ctttgtcgag caggctggag atcacgccct
      241 cgtcgacttc ccccgaggag ttgtagacag ccgctccgtt tgcgtcgagc acgaccacgt
      301 gaggaatgta gccgcggtag tacttctttt gcagttgctg ctgtgcgggt gaaagagcgt
      361 ggtcgaggtc gatcacgacg aactgcacgg ggcctttgta tttcccgtag aggctcaccg
      421 tgcgacgcgc ctggcgcttg gagttgtagc agccctcgcc gtacaggatc acgtaggagg
      481 gcttgccggc aaccgggccc gcgtccatgc tgtcgccggt gatgagcggg ccgtcctggc
      541 tgtcgctcga gtagtcgaga tggggattca gccttgaggt tgtccgctcc gaacgagagc
      601 gccggcgaaa cgaagcctag aatcaccagc attgcgggct ttctcattgt cagcctttcc
      661 accgactcac gctccaaact gccgcaggtg atggtccgcg tgcaggtatc ccagccgcat
      721 ccacgcggtc tcggacatct ggccgaagtg cgggtggggc tgatactcaa agtcgatcgg
      781 ccggcgcgtg aagcgatcga tcaagttaca caaatctctt cggtcggcct cgaactgcaa
      841 tgggggagtg ccgcctttct cctgatccag ctcgggcgcg gccttgaatc catgcggcca
      901 cggaatcgga gcccagatcg ccacaaactt catcagcgag cgcgggtacg gaactgcttc
      961 cggctttaca ggtctaacac ccatgaacat gcgataggaa tcactgagat ggcagagcat
     1021 ctggtgaggc gtcatcctgc cccagcggcg ctcgctgttg ggtcggatgg cctgtacgcg
     1081 ctgcagaact tcctgcttgt ctcgcagact gcggagggtc ttcatgttgg atgagttttg
     1141 tgccagcaca tgagagtgcc agcgtgcaac aaaagtttca gaaaacggtc gggagaaaag
     1201 tgagagcggg acaatacaaa acagcgccaa ctcggtgatg tcagacgccg accggaaatg
     1261 gcttcgtgtg agaggtcgcg gtgagggcgc gtccgccaaa gcggccctgc agcagcgaca
     1321 tcaagcccgt gtaccagtag ccgaccacga agagcagaag gaacgggacg gtgatgtagt
     1381 tctcgttgtc gaccgcgtag tagaccgtga gcgcgaaata gccgccaatc agcaactcca
     1441 cccacggcac ccaccccagg cgcttgcggt atttggtcgc gccgatcttg tcctgcttcg
     1501 tgatcacgcg gtatttcggg gtgcgcgcaa aggcgctctt cttgccgacg agcgcctcga
     1561 gcaccgcctt ggtgttggtg atggtcaggc cgattccgag cgccatcaga aacggcagat
     1621 acagcaaggc ccgcggccag ctccgcggaa acagctcgcg ctgcgagacc aggtaaaagc
     1681 tcgagatcga aaacgtcgac gccataaaca gcggcaggtc gatgtagagc atctggaacc
     1741 agccctggta gaagcggatg atcatggccg gcagcagcag cactgaaagc atgatcatca
     1801 ggggatagct gatgtttgcg gtcaggtgat accaggcttc gattttggtg tgcagcgggg
     1861 catcgctgcg cagcacgcgg ggcagaatct tctttcccgt ctggatcagg cccttggccc
     1921 agcgtgcctg ctgcgtcttg aacgcagtca tttcgacggg cagttcggca gggcattcca
     1981 catcctgcag ataaataaac ttccatccct tgagctgtgc gcgataggaa agatcggtgt
     2041 cttccgtgag cgtgtcgtgc tgccagccgc cggcttcctc gatcgtcttg cggcgccaca
     2101 tgccggcggt tccgttgaag ttgaagaaga gcccggtgcg ggcccggccg gaatgttcga
     2161 gcacgaaatg accgtcgagg aggatggctt cgacttgcgt caggaatgag tagccgcggt
     2221 tgaggtgcgt ccagcgtgtc tgcaccatgc cgatcttcgg atcggtgaag tggtggatcg
     2281 tgcgcagcaa aaagtcttcc ggcggggtga agtcagcgtc gaagatggcg atgaattcgc
     2341 cctccgctga cttcatgccc tcggcgagcg cgcccgcctt gaaaccttcc cgattggtgc
     2401 ggtggtgata gctgatgggc tggcccagcg cggcatagcg ctcgaccaag ccgcgagcga
     2461 cttcgacggt ctcgtcggtc gagtcgtcga gcacctgaat atcgagcttc tcgcgcggat
     2521 aatccaggcg acagatcgcg tccagcagcc gctcgaccac gtattgctcg ttgaagatcg
     2581 gcaattgcac ggtgacacgg ggcagatctt caaacctggc agtgggctcg gtggtgcggt
     2641 tcttgctgtg cttgtagtag aggtagacca gaatgtagcg atgcgcgccg tatccggcca
     2701 gaagaatgag gacgacgaaa tagggaatca gcaacgcgag gtcgaacccg ttgacgtggt
     2761 agagcccgcg gaacgtggtg tcgaggaagt ggtcgcggag gtagcgggcg acgcctcggg
     2821 aggcgatcca gatggcgaac gaggctgagg cgtgcgcggc tgcgatgaga tcagaaatca
     2881 gcgagtactc ctgtggaaac ctctcatttt acagcattgg taggtgggtc ctacccggcg
     2941 tcatcccgaa ggcccgcgat ttctccagcg ggccgaggga tctccctagc cgccgcactc
     3001 tacaggagat ccttcgcagc gcctgaaaag cggctccgct caggatgacg ccgctctccc
     3061 agcggtgtgc aatgcggaag agaaattttg cccttgacat attcccatat cggaatatat
     3121 attcttgcct atgccacgag ccgctaccac ctccgacgcc ttcaacgccg tagccgaacc
     3181 ccggcggcgt cagatcctcg atttcctggc gacgcaggag cggccggtgg gcgacatcgt
     3241 cgcggggctc aggctcgagc agccgtcggt ttccaagcac ctgggcgtgc tgcgcagcgt
     3301 ggggttggtg cgggtgcggc gaaacggacg ccacatgctg tatcgaacca atgccgaagc
     3361 catccgcccg ctgcatgagt ggacaaagac ttttgagcgc ctgtgggcgc atcaactgac
     3421 gcgcatcaag gagcgcgccg agagcaagac tgcagaacaa aacagaagcc atgaaaagga
     3481 ggaagcatga gcgcaatcgt caccccgccg ggaattgaga gcttgacgct ggtcatcacc
     3541 cgggaaattc acgtaaaggc aacgcttgag aacaccttcg ccgcgattct cgaacagatc
     3601 ggtccgtaca acgaaacgcc ggaagggccg atgcccatga agatcgagcc ctggccgggc
     3661 ggccgctggt accgcgacct gggcgacaac aacggacact tctggggcca tgtgcaggcg
     3721 atcaagcgac ctacgctgct cgaaatcacc ggcccgctgt tcatgtcgta tgccgtggtt
     3781 tcgaacctcc agtaccggct gagcgaggcc gacggcggca cgttgatcaa gtttcatcac
     3841 aaggcgttcg gtgtcattca ggacgagcac cggcgcggcg tcgtcaaggg ttgggatcac
     3901 attctcggtt ccattcgcga ccgcgcccag gcgagcacga agtaagttcg caggaattta
     3961 gaagttcaga gcag
//
LOCUS       DASPUL010000552         4881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1877931, whole genome shotgun sequence.
ACCESSION   DASPUL010000552 DASPUL010000000
VERSION     DASPUL010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1877931"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..1480
                     /locus_tag="VEJ00_12880"
     CDS             425..1480
                     /locus_tag="VEJ00_12880"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HXX02103.1"
                     /translation="MESVIGSFSRCGCVLALALAVVALARNRAAAQPPQGTVDASQNS
                     PQPFPDAAQPAKEPGNDPGKGSDDKANPASLAKDSSDASLVKLGPGDLIDMSVFNVPE
                     LTTKARVSNSGDVYLPLIDYVHIAGLTQEEAQTVIEKRLDEGGFVRNPHVTIFVDEAS
                     SQSVTIIGEVGKPGIYPDPINRKLYDIISEAGGFTLSASRKIVIIRNDKPMHIDLPRN
                     LGDDLGGNVEVLPGDTITVPRAPIIYVVGDVGRPSGLLVDNGALTVLQALALAGGANH
                     TAKLSGARIIRKTPTGMTETTVQLKKMLEAKTADVPLQADDILFIPVSGGRVLAARTM
                     EVAMSAATAVTIYSVHP"
     gene            complement(1513..2790)
                     /locus_tag="VEJ00_12885"
     CDS             complement(1513..2790)
                     /locus_tag="VEJ00_12885"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXX02104.1"
                     /translation="MKILYVSQYFPPEMGAPAARADELARHWADAGHEVSVLTGFPNH
                     PTGVVPEEWRPRLRRLVYHEKRNGVDVFRTWLWPLPNRKAHERMRNYASFCVSGALRG
                     LFLPRPDVIIATSPQLLVALAGWWLAFARQLPFVFEVRDLWPESLTAVGIGNENSLLH
                     RALAGIAGFLYERADRIVVVSPAFREYLVQRWRVPAEKIAVVENGVETDMFSPQPVAA
                     DQALRRELGADGNFLVCYVGTIGMAHGLETLLDAADQVRQQDPSVLFLIIGEGAEKQR
                     IRTLAQSRSLANVRFLDQQPRERIPAFISASDACLVLLKKTEVFKTVIPTKMLEFMSC
                     ARPVIVGVDGQARQIIEDAHAGLAIEPENAEALVGAIRHLAADRALGVKVGQKGREYV
                     LRHFSRGQTAAKYIEILQQEVGKPHEGGAKAIA"
     gene            complement(2865..3836)
                     /locus_tag="VEJ00_12890"
     CDS             complement(2865..3836)
                     /locus_tag="VEJ00_12890"
                     /inference="COORDINATES: protein motif:HMM:NF018378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HXX02105.1"
                     /translation="MPSLAYYRRIFSAYLGGGQSQLTFWHETPAENPHFVPGELGEYY
                     MLFAEKADYCGAYDASGVPQLDYRGSIGLQYNPIAIAQYGLGNYNFFRRSGDRTRREK
                     FFLTADWLCSRLENNAHGLAVWNHHFDWEYRDTLKAPWYSALAQGQGISVLLRAHQES
                     GDARYHDAAARALACFFHPVDKGGVAFTDESGDLWFEEYIVSPPTHILNGFIWATWGI
                     YDYWLATKDVSAQELFSRAVRTLLHNLDRYDLGFWSLYEQSGTRLPMVASRFYHQLHI
                     VQLRVMHRLTGEAAFARVADRWEGYAKSRVNRARALGYKSAFKLCYY"
     gene            complement(3991..>4881)
                     /locus_tag="VEJ00_12895"
     CDS             complement(3991..>4881)
                     /locus_tag="VEJ00_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02106.1"
                     /translation="LRARLSSTNRMTARLFRDGFHALAVLPSGGLVAAVAGAIVTLRA
                     GEREFVQTHAISRGTRPLHITAVPGGTVYWGEYFDNPSRDEVHIYASTDAGLTWNVAY
                     TFPKRGIRHVHNIVHDPWEDCLWVLTGDYGDECRILRASCDFTHVEAVLQGNQQARAV
                     ALVPTEGGLYFSSDTPLELNYIYRLDRQGALTRLTAISSSSIYGCRVGGQVFFSTMVE
                     PSEVNRDGHVRIYGGRSGDGEPSWRPVLAWRKDRWPMGLFQYGNAFLPDGVNTTRCLA
                     VSTIAVESDDMVTSLYPVGG"
BASE COUNT         1044 a         1397 c         1537 g          903 t
ORIGIN      
        1 tggacgtggc gctcttgcaa aggcacggcc acccagccgt gggcggttgc ggtatcgcgc
       61 acgtagcggg tcagcacgaa ggcggacagc agcgccagac aaaacgtcga cagaagaatt
      121 tccgtcaact attccccctc ggcaagtcga caccccggca ggattcgtcg gccggagggc
      181 ccgtcataaa gtatgcggcg acgagcggcg gtaaaaaagt aaacgatcag attcctaaac
      241 ggggcttaga gtgcagaaaa ctctagcagt acaagagcgc gacttgcaat cgccagcgag
      301 gaaaaacctg tggaaaacta attctccccc gcgtaaatca agggggcacg agggatagcg
      361 ctgtgctaaa cttttgctca aattctcatt gaaactgcgc tcaaatgcgc caggggtcct
      421 ttccatggag tcggtcatcg ggagctttag ccgctgcggt tgcgttcttg cccttgcctt
      481 ggccgtggtt gcacttgcca gaaaccgcgc ggcggcgcag ccgccgcagg gaacggtgga
      541 cgcatcccaa aacagcccgc agccgtttcc cgacgctgcc cagccagcca aggaacctgg
      601 gaacgatccc gggaaggggt cggacgacaa agcaaacccg gcatcgctgg cgaaggacag
      661 cagcgacgcg tctttggtaa agcttgggcc cggcgatctc attgacatga gcgtatttaa
      721 cgttccggaa ctgaccacca aggcgcgagt aagcaattcc ggcgacgtat atctccctct
      781 gatcgactat gtgcacatcg cgggcctgac ccaggaagag gcgcaaacgg tcatcgagaa
      841 gcggctggac gaaggcggat tcgtacgcaa tccgcacgta accatcttcg tcgatgaagc
      901 aagttcgcag agcgtgacca tcattgggga ggttggcaaa cccggtattt accctgaccc
      961 gatcaaccgc aagctgtacg acattatttc tgaagctggc gggttcacgc tgtcggcctc
     1021 ccgcaaaatc gttattatcc gcaacgataa gccaatgcac attgatctcc cacgcaacct
     1081 gggagacgat ctcggcggta acgttgaagt gctgcccggc gacactatta cggtgccccg
     1141 tgcaccgatc atctatgtcg taggggacgt gggccggccc agcggcctgc tggtggacaa
     1201 tggcgcgctc accgtgctgc aggctctggc tctcgcgggc ggcgccaacc acacggcaaa
     1261 gctcagcggt gcgcgcatca ttcgcaagac accgacgggg atgaccgaga ccacggttca
     1321 actcaagaag atgcttgagg ccaagaccgc agatgttcct ctgcaggccg acgatattct
     1381 gttcatcccc gtcagcggag ggcgcgtgct tgcagcaaga acgatggagg ttgccatgtc
     1441 agcggctact gcggtcacga tctactcggt acatccgtag aggtacgcag agccaatgcc
     1501 cggctagaaa gttcaggcga tggctttggc gccaccttca tgcggctttc caacttcctg
     1561 ttgaagaatc tcgatgtact tcgccgcggt ttggccgcga gagaaatgac gcagtacgta
     1621 ttcccttccc ttttgtccca ctttcactcc cagtgcgcga tctgcggcta ggtgccggat
     1681 ggccccgacc agagcctctg cattctctgg ctcgatcgcc agtccggcgt gagcgtcttc
     1741 gataatctgc cgagcctggc catcgacgcc gactatgacg ggacgagcgc aggacatgaa
     1801 ttcgagcatc tttgtgggga ttaccgtctt gaaaacttcg gtcttcttga gcagaaccag
     1861 gcatgcgtca gaagcggaga tgaaggcggg gattctctca cgcggctgct ggtcgaggaa
     1921 gcgaacgttg gccaggctgc gcgattgggc caaagttctg atcctttgtt tttcggcgcc
     1981 ctccccgatg atgagaaaca gcacggaggg atcttgctgt cgcacttgat ccgcggcgtc
     2041 cagcagcgtt tccaggccat gtgccatgcc gatggtgccg acatagcaga ccagaaaatt
     2101 gccatcggcg ccgagttcgc gacgcagagc ctgatcggcg gcgacgggct ggggcgaaaa
     2161 catgtcggtt tccacgccgt tttcaacgac agcaattttc tctgcgggaa cgcgccagcg
     2221 ctgaaccaga tattcccgaa aggccggcga gacgacgacg atccggtcgg cgcgctcata
     2281 gaggaatccg gcgattcccg ccagcgcgcg gtgcaacagg gaattttcat tgccaatgcc
     2341 tacggcggtc agcgattcgg gccagagatc gcgcacctcg aagacaaaag gtagttgcct
     2401 ggcaaacgcg agccaccatc cggcgagcgc aaccagaagt tgcggcgagg tggcaatgat
     2461 cacgtccggg cgcggcagga acaggccgcg cagcgcgccg gagacgcaaa acgatgcgta
     2521 attgcgcatg cgctcatgcg ccttgcggtt gggcaggggc cagagccagg tgcggaagac
     2581 gtcgacgccg ttccttttct cgtggtaaac caggcggcgc aggcgggggc gccattcttc
     2641 cggaaccacg ccagtcgggt gattgggaaa gccggtcagc acagagactt cgtgtccggc
     2701 atcggcccaa tggcgggcga gttcgtcggc acgggcggcg ggagcaccca tctccggcgg
     2761 aaagtattgc gagacgtaga gtatcttcac tggaggtaga ggctatcacg ctgactcctg
     2821 gagttggcca tatggagcgc attacgactc gaactgatct gaacctagta gtagcagagc
     2881 ttgaacgcgc ttttgtaacc cagcgcccgg gcgcggttga cgcggctctt ggcgtaacct
     2941 tcccaccggt cagcgacacg ggcaaacgcg gcctcgccgg tcaggcgatg catcactcgc
     3001 agctgcacaa tgtgcagttg atgatagaaa cgactggcaa ccatcggcag gcgtgtgcct
     3061 gactgctcgt aaagggacca gaatcccaga tcatagcgat caagattgtg cagcaacgtc
     3121 cgaaccgctc gggagaacaa ttcctgcgcc gacacatctt tggtcgcgag ccagtaatcg
     3181 tagattcccc acgtcgccca gatgaatcca ttcaggatgt gcgtcggcgg ggagacgatg
     3241 tattcctcaa accacaggtc gccggattcg tcagtgaacg ccactccgcc cttatcgacg
     3301 ggatggaaga agcaggcgag agcccgcgcg gcagcgtcgt gataccgagc atcgcccgac
     3361 tcctggtggg ctcgcaacag cactgagatt ccctgccctt gggcgagagc cgagtaccac
     3421 ggggccttca gcgtgtcgcg gtactcccag tcgaagtggt gattccagac tgccaggccg
     3481 tgggcattgt tctcgagccg ggagcagagc cagtccgcag tcagaaagaa cttctcgcgc
     3541 cgggtgcgat ctccgctgcg gcgaaagaaa ttgtagttgc ctaaaccgta ctgcgcgatg
     3601 gcgatggggt tgtactggag gccgatacta ccccgatagt cgagttgggg aacgccggaa
     3661 gcgtcgtagg cgccgcaata gtcggccttt tcggcaaaca gcatgtagta ctcgcccaat
     3721 tcgccgggaa caaagtgggg attctcggct ggcgtttcat gccagaaagt gagttggctc
     3781 tggccgccgc caagatatgc gctgaagatg cgtcggtagt aggcgaggct gggcaaggga
     3841 tttcgcgaac gcgcagtcca tattacggca aggttgtgag atgggttgga gtgggcggtt
     3901 gactgcctgg ctttcgcgtc cgcacccggc gagcttcgct cgaccggaca gccgggggcg
     3961 gctgtcccta catgtgtgta tttcaggcga tcagccgccg actgggtaga gcgatgtaac
     4021 catgtcgtct gactcgacgg cgatagtact aaccgcgagg cagcgtgtgg tattcacgcc
     4081 atccggcagg aaagcgttgc cgtactgaaa cagccccatg ggccagcggt ccttgcgcca
     4141 ggcaagcacc gggcgccagg aaggctcgcc atcgccagat cggccaccat agattcgcac
     4201 gtggccgtcg cgattaactt cgctcggctc gaccatggtc gagaaaaaaa cctggccgcc
     4261 cactcggcaa ccgtagatgg aggaactgct gatggcggta aggcgcgtca aggctccctg
     4321 acgatcgagc cggtagatgt agttcagttc gagaggggta tctgaggaga agtacagtcc
     4381 gccctcggtg gggaccagcg cgacggcgcg agcctgctgg ttgccctgga gtacagcctc
     4441 gacatgggtg aagtcgcagg aggcgcgcag gatgcggcat tcgtcgccgt agtcgccggt
     4501 cagaacccag aggcagtctt cccatggatc gtggacgatg ttgtgaacgt ggcggatgcc
     4561 acgtttggga aaagtgtagg cgacgttcca agtcaggccg gcatctgtcg aggcgtagat
     4621 gtgaacctca tcgcgagatg gattgtcaaa gtactctccc cagtacacgg ttccccctgg
     4681 caccgcggtg atgtgcaagg gacgagtacc gcgactgatt gcgtgagtct gaacgaactc
     4741 ccgttcgccg gcgcggaggg tgacgatcgc accggcaacc gcggctacca atccaccgga
     4801 tggaagcacc gcgagggcgt ggaagccgtc gcgaaacaga cgcgcggtca tgcggtttgt
     4861 ggaggatagg cgtgcccgta a
//
LOCUS       DASPUL010000553         5186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_245678, whole genome shotgun sequence.
ACCESSION   DASPUL010000553 DASPUL010000000
VERSION     DASPUL010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245678"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VEJ00_12900"
     CDS             <1..604
                     /locus_tag="VEJ00_12900"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXX02107.1"
                     /translation="CRAKFPAVEFIITDASDLSGFPDSCFDAVVFAFNGIDLLLPDKT
                     RRACLTHMHRVLKPGGVVIFSSHNARAVLSWPNWNRERLEQMARRGSAGSRGLERLLQ
                     VFLTLVRSTLAFGQALGASLLRLLRRLPLRAFWSGEGTFVDSAHGGLLTHCWTPGFAV
                     RELSEAHFQILQIVGNDYPRASHRFATDWYYYVFAKAGDR"
     gene            655..1749
                     /locus_tag="VEJ00_12905"
     CDS             655..1749
                     /locus_tag="VEJ00_12905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX02108.1"
                     /translation="MRQQWNTLVERVDHPQVFYTYEWSLAAQRAYHVILRPMIFLAYD
                     ERNALCGVASLALDLEGKKVSFLCATTGDYCDFISLAEHRTAFVGGVLAELRRLGIGD
                     LTLTNLPADSATVAALRSAAGRQGYRWFARVAYFCAQVSLGKLERRPGDHKPVLPGRK
                     MVRRSLNAMRQDSPVRLDHARSWDAIAPILPQFVRAHRARFRAIGRSSNLARPERLVF
                     LEELAKLLSESGWIVLTRMMSSDKVLAWNYGFESHGTWFWYQPTFENEFERYSPGFCL
                     LSMLIEEAAENPALSVVDLGLGEDEYKQRFANQSRKTLYVTLRSSMAKHLQEILRYRA
                     TELIKTSPRLEAGVRSFFSRLRKNTESDLQ"
     gene            1859..2971
                     /locus_tag="VEJ00_12910"
     CDS             1859..2971
                     /locus_tag="VEJ00_12910"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXX02109.1"
                     /translation="MSSEPMNTPANGAEAKSSADSPVFIVGMSRGGTTLLSRMLDAHS
                     DIAILPETWMYVVLDRLGCLEEFSNPWQTALFFNEVWKNLASYRDPAARVVALEAAKE
                     RGYLGPSARLLEQIGRAYAKERNAKIWGEKTPGHALWLPQIRLLFPQARILFMVRDPR
                     DVLVSYDERWDEGRRDTKYLVSTASLLKFYMSHLLRRPAFPPEQVQWVRYEALAANPA
                     AELQRVCNFLGVDFQQSMLAFYRRHANVEHEMPDGRHHALLSRPVTTEKIGRYQEALT
                     QTQIALVESLLSEEMQSVGYSPSNSHLAPLTHIEKRALSEAEEYYRQMVSGEIRRRFR
                     RRGRLKVRTYQIFGRALGIVPSWRVAVSENDWRRRC"
     gene            3178..3471
                     /locus_tag="VEJ00_12915"
     CDS             3178..3471
                     /locus_tag="VEJ00_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02110.1"
                     /translation="MLVSSETPQLADNTSQGLCPDQRELVEVGGIDDEEFPLDMPAYY
                     DWMLQPSSRHRYRRIVAFIGVMVTLLIALSALIVYSSSTVPPSVFVSPVSSSH"
     gene            3996..>5186
                     /locus_tag="VEJ00_12920"
     CDS             3996..>5186
                     /locus_tag="VEJ00_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02111.1"
                     /translation="MLLVPEVLTALTVERIAAEISPVAELWATRNGFPASLFGIALER
                     PVRSFGESVLARVVASSGRYAGLVSRFSSAQIKEIILDKYDASYRWRARFAARPERCR
                     SSVVVLPSAYANVSRTAAAYARLLPAQSFLLVATRQSAKQVIAPANVQLRDLAAYAKR
                     AAAVEMVCLTDRWNKVASDLCSIPELRTLFRAGVLDRIPVWLRDGLCARDAWRGVIEG
                     EPVSGVLCGDDSNLYTRLPVVLAARRGIPTADFHHGALDGRYLLKDLPCDVYLAKNEM
                     ERDYLLRVCNIPGERVVIGAPPHSQLGLRAEHTEPRRNSAIFFSEPYESAGMRGEEVY
                     REILPALCRLVRQNGRELILKLHPFEIASHRNKILHDVLTPADRKQITVIAGPLTNDL
                     ISHAW"
BASE COUNT         1007 a         1462 c         1561 g         1156 t
ORIGIN      
        1 ttgccgcgcg aagtttccgg cggtggaatt catcatcacg gacgcgtcgg acttgtcggg
       61 ttttccggat agttgctttg acgccgtagt cttcgcgttc aacggaatcg acctcctgct
      121 gcctgataag acgcgacgag cctgtctgac gcacatgcat cgcgtgctca agccgggcgg
      181 tgtggtgatc ttctcctcgc acaatgcgcg ggccgtcctg tcgtggccga actggaaccg
      241 cgagcgactg gagcagatgg cgcgccgagg ctccgccggt tctcggggac tggaacgctt
      301 gcttcaggtt ttcctcactc ttgtccggtc aacgctcgcc ttcggccagg ccctcggcgc
      361 ttccttgctg cggctactgc ggagattgcc cctacgtgcc ttctggagcg gagagggaac
      421 ctttgtcgac tcggcccacg gcggcttgct cacgcactgc tggactcctg gctttgctgt
      481 tagggaactc agcgaggcgc atttccagat tctgcaaatt gtagggaatg actatccccg
      541 cgccagtcac cgcttcgcga cggattggta ctattacgta ttcgccaaag ccggcgaccg
      601 gtgaatgcag acttgcgatt cgatcggctc agagagattc cggaggatgc gagcttgcgc
      661 cagcagtgga acaccctggt ggagcgtgtc gatcatccac aggtgttcta cacctatgaa
      721 tggtcgctcg cggcgcaaag ggcctatcac gtgattctcc ggccgatgat ttttctggcc
      781 tatgacgaac gaaatgcctt gtgcggtgtc gcttcgctcg cattagacct ggagggcaag
      841 aaagtttcgt ttctgtgcgc gaccacaggg gactactgcg acttcatcag cttagccgag
      901 caccggacag cattcgtcgg cggagtgctg gcagaattgc ggaggctggg cattggcgat
      961 cttaccctta ccaacctgcc cgctgattcg gccacagtcg ccgctctgcg gagtgcagcg
     1021 ggcagacagg gctatcgctg gttcgcgcgc gtggcctatt tctgcgcgca ggtgtcgctg
     1081 ggcaaactcg aacgtcgccc tggggaccac aagccggtct tgccgggaag aaagatggtg
     1141 cgacgatctc tcaatgcaat gcgccaggat tcgccggtgc gcctcgatca tgcgcgctcc
     1201 tgggatgcga tcgctccaat tctccctcaa tttgtgcggg ctcaccgggc acggtttcgg
     1261 gctataggtc gaagcagcaa tctggcccgc ccagaacggc ttgtattcct cgaagaattg
     1321 gcgaagctgc tttcagagtc aggctggatt gttctcacgc ggatgatgag cagcgacaaa
     1381 gttctcgcct ggaactacgg tttcgagtct catggcacct ggttctggta ccagcccaca
     1441 ttcgaaaacg aatttgagag gtattctcca gggttttgcc tgctctcgat gctgatcgag
     1501 gaagcagcgg agaatcctgc gttgagcgtc gtcgaccttg gcctgggaga ggacgagtat
     1561 aaacagcgat tcgcgaatca aagccgcaag acgctgtatg tgactctgag aagttcgatg
     1621 gcgaaacatc tgcaagagat tctgcgctat cgggctacgg agctcatcaa gacttctcct
     1681 cgactggagg caggcgtccg cagctttttt tcccgcctgc gaaaaaatac ggagagcgat
     1741 ctacaatagc ctgctgagtt ctaaccgaca gccacgtcga aaaccgggaa gaacgtctgg
     1801 actagcgcat tgctccctgg aaaatggcgg agtccgagaa cgcagactga ggattttctt
     1861 gagctctgaa ccgatgaata ctccggcgaa tggggcggaa gcaaagtctt ccgccgactc
     1921 acccgtcttc atcgtgggta tgagtcgcgg tgggaccact ctgctgtctc gaatgctgga
     1981 tgctcattct gacattgcga ttcttccgga gacctggatg tacgtggtcc tggatcgcct
     2041 cggttgtctt gaagaattca gcaacccttg gcaaacagca ctgtttttta acgaagtctg
     2101 gaagaacttg gcctcctatc gggacccggc ggcacgagtc gttgcgcttg aggcagcgaa
     2161 ggaaagagga tatctgggac cgagcgcacg actcctggag caaatcggac gggcctatgc
     2221 gaaggaacga aacgcgaaga tttggggcga gaagaccccg ggacacgcgc tgtggctgcc
     2281 acagattcga ttgctctttc cgcaagctcg catattgttc atggtgcggg accctcggga
     2341 cgtcctggtg tcatacgacg agcgttggga cgaagggcgc cgggacacta agtatcttgt
     2401 tagcactgcg tcactgctca agttctacat gagtcatctc ttgcgccgtc cggcatttcc
     2461 tcccgagcag gttcagtggg tcaggtacga agcgctggcc gccaatccgg ccgcagaact
     2521 ccagagggtc tgcaatttcc tgggagttga tttccagcag tctatgctgg ctttttaccg
     2581 tcggcacgcg aatgtggagc acgagatgcc ggacggaagg caccacgcac tgctgagccg
     2641 gccggtaacg accgaaaaaa tcggacgtta tcaggaggcg ttaactcaaa ctcagatcgc
     2701 cttagtggaa agtcttctga gcgaagaaat gcaatctgtg ggctactcgc cctcaaactc
     2761 tcaccttgct ccgttgaccc acatcgaaaa gagagctttg tcagaggccg aggaatacta
     2821 ccggcaaatg gtatccgggg aaattcgaag gagatttcgc cgtaggggca gattgaaggt
     2881 acggacttat cagatcttcg ggcgcgcact cggaatagtg ccctcttggc gggtcgccgt
     2941 ttccgaaaac gattggcggc gacgctgcta agccgggcat cgatgttcgc gcaagctcga
     3001 ttggcagaaa tcgtagtgaa ttgtgctctc gcgcccacat tgtggtgtcc gcagcacacg
     3061 agcgtcttaa gccttgttca agtcatcctt cacctggact accaggactg agagtccgct
     3121 tccccgaacc aacagagata ctgtcaagga cgaccgccgc aatcctagga ttgagacatg
     3181 ttggtctcgt ccgagacgcc gcaactcgcc gacaacacct cacagggatt gtgtccagat
     3241 caaagggagt tggtcgaggt cggtggcatc gatgatgaag agttcccgtt ggatatgccc
     3301 gcttactatg actggatgtt gcagccttct tcccggcacc gttataggcg gatagtagcg
     3361 ttcatcggtg tgatggtgac tctgctcatc gcactctcag cacttattgt ctacagttct
     3421 tcgactgtgc cgccatcggt gttcgtctca ccagtatctt cctcgcacta gaactcatgc
     3481 tgagggcgcc gttgcttttg gacgacgggc atagttcctt cccggcacgc cttaatctgt
     3541 gccgaaccga caattcctgc taggctcttc accagtgctc ttggtttgcg atgcccctgg
     3601 ttgacctgtg tgtcgccgtc gaggaaaaat acggccacgt attcggcagc gtggcaaggg
     3661 cgcgatgcgc cgctccgtga tcctcctggc cgccgaagct tctgcgcgga ttccacggtc
     3721 cgcaacgcaa tatcatgcgc gttctgctcc tccatcccga agactcgcca cgtcgggggc
     3781 cctggacccg gcagcgctgg gacctggttg ttgacctcgg caaatcctcc catttctcag
     3841 aagaagactg ggcgcggctg tatggctgtc cggtttggcg cgcggatttg tttcgccagg
     3901 gaattgcgga tgtgaggcga gtgcgtcaaa tattctcatc gggccgaggt cgattggtcg
     3961 atggagaggg aatcgactgg tgggaatttt tatccttgct gctggtaccg gaggttttga
     4021 ctgccctgac cgttgagcgg attgcggccg agatcagccc ggttgccgag ctttgggcta
     4081 cgcgcaacgg atttccagcc agcctctttg ggatagcgct cgagcgtccg gtgcgcagct
     4141 tcggggagag cgtgctggct cgggtggttg cttcctccgg gcgctacgcg ggtctagtct
     4201 cccggttctc gtccgcacag atcaaagaga tcattctgga taagtacgat gccagctacc
     4261 gctggcgggc acgcttcgcc gcgcggccgg aaagatgccg aagctctgtc gtggtgctgc
     4321 ccagcgcgta cgcaaacgtt tcgcggacag cggcagccta tgcgcgactg ctgccggcgc
     4381 agtcgttcct gctggtcgcg acgaggcaga gcgcgaagca ggttatcgcg cccgcgaacg
     4441 tgcagttgcg cgatctcgcc gcctatgcga aacgcgccgc ggccgtcgaa atggtatgcc
     4501 tgaccgaccg ctggaacaag gtagcctccg atttgtgttc gatcccggaa ttacgaaccc
     4561 tcttccgtgc cggggtactg gatcggatcc ccgtgtggct gcgcgatggc ctctgtgccc
     4621 gcgatgcgtg gcgcggagtg attgagggcg aaccggtgag tggcgttctt tgcggcgacg
     4681 acagcaatct ttacacaagg ttgccggttg tgctggctgc gcggcgggga atcccaaccg
     4741 ctgattttca tcatggcgcg ctggacgggc gttacttgct gaaagactta ccctgtgacg
     4801 tataccttgc taagaacgaa atggaacggg actatttgct gcgggtctgc aatattccgg
     4861 gggagcgggt ggtcatcgga gcgcccccgc attcgcagct tggcctgagg gcggagcaca
     4921 ccgagccgcg acggaactca gccattttct tctccgaacc ctacgagtcc gcaggcatgc
     4981 gcggcgaaga ggtctatcgc gagattttgc ccgctctctg tcggctggtg cggcagaacg
     5041 ggcgtgagct gatcctcaaa ctgcatcctt ttgagattgc ctcccaccgc aacaaaattc
     5101 ttcacgacgt cctgactccc gctgaccgga agcagatcac agtgatcgct ggccccctta
     5161 ccaacgatct gatctcacac gcttgg
//
LOCUS       DASPUL010000554         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1878233, whole genome shotgun sequence.
ACCESSION   DASPUL010000554 DASPUL010000000
VERSION     DASPUL010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1878233"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1937
                     /locus_tag="VEJ00_12925"
     CDS             <1..1937
                     /locus_tag="VEJ00_12925"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HXX02112.1"
                     /translation="DKSSARPQSGASTLRWYLSRLASMQPAEVFWRVRSAATLPLDWA
                     QWKRQPAVPAPRWTPLDSASYPVRLHQSGQAMEHIRVFDLEFPLGFEFDWHRDYRNGR
                     LVERSFAGAMNIRDTAVVSDIKYVWEPSRHQHLSALAFAANGEEHAGYIVRALDSWLH
                     ANPYLSGVHWTSSLELAERVISWALLYPRIADIVAADQDFRRRWLDSIYLHLRRIARK
                     LSLYSSANNHLIGELVGLFVGASCFDFWPECAGWRKLSREMLEREIRLQVGEDGVDRE
                     QAMSYHLFTMELFLLAFAIGRNTGSPFSDAYAQRLRGMARFVDTVATATGDLPWYGDS
                     DDARGFVLSESQSGLQVTTQLAGLLFDEPRWLRFSDTATEAARALVPGLLPRLDRVAA
                     APAAPRELFPDCGLACLHTNDGKVRLLMDFGPHGYPSTAAHGHADALSIWMAIGEEYF
                     LVDAGTYAYHSHPEWRNFFRGTSAHNTARVDGRNQSEMAGRFLWSTKANARLLRFENV
                     AGKATIEAEHDGYARLPQPVIHRRTVEFDRTTGNVSLADSFQCSGRHEIELFFHMHEA
                     AEVVSVSDGEAKVEWRGRRIVFSSPDRHMRWEILRGSDEPQLGWRSRRFNHKQPIATL
                     RVRAEIDGSSTIRTDLRINS"
     gene            1934..>2875
                     /locus_tag="VEJ00_12930"
     CDS             1934..>2875
                     /locus_tag="VEJ00_12930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HXX02113.1"
                     /translation="MKISIVGLGYVGAVSSACLASAGHRVWGVDINPEKVRIINEGAS
                     PIVESGLAEKIAQARSAGRLQATVDIAEALRGSELCFVAVATPSRPNGEIDPAHLFRA
                     CKQIGTALATLRRKQIVVIRSSVLPSIFDECRRIFASNAPGLVELCANPEFLREGTAI
                     LDFEEPPFTILGTDTAAAEDMLRSLYADQTAPIFVLKPREALMVKYASNAFHALKVAF
                     ANEIAAVCQPAGVDADAVMNIFCSDTKLNISRRYLKPGFAFGGSCLPKDVRAILYAGK
                     GMDLQLPLMAAILSSNEEVIERAFQKVRATGKRRIGMI"
BASE COUNT          515 a          892 c          903 g          565 t
ORIGIN      
        1 aggacaagtc gtccgcgcgg ccgcagagtg gcgcatcgac gctgcgctgg tatttgtcac
       61 ggctcgcctc gatgcagccg gccgaagtct tttggcgtgt tcgttcggcg gccacgctgc
      121 cgctggactg ggcgcagtgg aagaggcaac ccgcagttcc ggctccgcgt tggacgccgc
      181 tggactccgc gtcgtatccg gtgcggctgc accagagcgg acaagcgatg gagcacattc
      241 gagtattcga tctggagttt ccgctgggat tcgaattcga ctggcatcgt gactaccgca
      301 acggacgcct ggttgagcgc agtttcgccg gcgcgatgaa catccgcgat acggctgtcg
      361 tcagcgacat caagtatgtc tgggagccga gccgccacca gcatctctct gcccttgcct
      421 ttgccgctaa tggagaagag catgccggct acatcgtgcg cgcgctcgat tcctggctgc
      481 atgcgaaccc gtatctttcc ggagttcact ggaccagcag cctggaactg gcggagcggg
      541 ttatatcgtg ggctctgttg tatccccgca tcgccgatat tgttgccgcc gaccaggact
      601 tccgtcggcg ctggctggac tcgatttacc tgcacctccg gcgcatcgcc cggaagctga
      661 gcctgtattc ctcggccaac aaccatctga tcggcgaact ggtaggcctg tttgtgggcg
      721 cgtcgtgctt cgatttctgg ccggagtgtg cgggctggcg caagctttcg cgggagatgc
      781 tcgaacgaga aatccgcctg caagtcgggg aagacggcgt ggaccgcgag caggcgatgt
      841 cctaccacct gttcacgatg gagctgttcc tgcttgcatt tgccatcggc cggaatacgg
      901 gttctccgtt cagcgacgca tatgcgcagc gcttgcgagg catggcgcga ttcgttgata
      961 cggttgccac tgccactggc gacctgccgt ggtatggcga ttccgacgac gcacgcggat
     1021 tcgtgctttc ggaaagccag agcggcctcc aggtcactac gcagctggcg ggactacttt
     1081 ttgacgagcc gcgctggttg cgcttcagcg acacggccac cgaagcggcg cgtgcgctcg
     1141 tccccggcct tctgccgcgt cttgaccgcg tcgcggcggc tccggctgcg ccccgcgaac
     1201 tgtttcctga ctgcgggttg gcctgcctgc acacaaacga cggcaaggtg cgcctgctca
     1261 tggattttgg tccgcacggc taccccagca cggcggcgca cggccacgcc gatgcgctct
     1321 cgatttggat ggccatcggt gaggaatatt tcctggttga tgcgggcacc tacgcctatc
     1381 actcgcatcc cgagtggcga aatttctttc gcggcacctc agcgcacaac accgcgcgcg
     1441 tcgacggtcg gaaccagtcg gaaatggcag gccgctttct gtggagcaca aaggcgaatg
     1501 cccgcctgct gcgcttcgaa aacgtcgcgg gaaaggcgac gatcgaggcc gaacacgacg
     1561 gctacgcacg gctgcctcag ccagtgatcc accggcggac ggttgagttc gaccgtacga
     1621 ccgggaatgt ctccctcgcc gatagctttc aatgttccgg ccgacatgag atcgagctgt
     1681 ttttccatat gcatgaagcc gccgaagtcg tcagcgtcag tgacggcgag gcgaaggtgg
     1741 aatggcgcgg gcggcgaatc gtcttcagct cgcccgatcg ccacatgcgc tgggaaatcc
     1801 tgcgcggctc tgacgaaccc caactgggct ggcgttcgcg ccggttcaat cacaaacaac
     1861 ccatcgccac gcttcgtgtt cgcgcggaga tcgatggcag cagcacaatt cgcacggatc
     1921 tgaggatcaa ttcatgaaga tcagcatcgt cggtttaggt tacgtgggcg cggtgtcgtc
     1981 cgcgtgcctg gccagcgcgg gacaccgcgt ctggggcgta gacattaacc cggagaaggt
     2041 acgcatcatc aatgaaggcg cttctccgat tgtcgagagc ggtctggcgg aaaaaatcgc
     2101 acaggcgaga agcgccgggc gcttgcaggc gacggtggac atcgccgagg ccctgcgtgg
     2161 gtccgagttg tgcttcgtgg cagttgcgac tcccagccgc cccaacgggg aaatcgatcc
     2221 cgcccatcta ttccgtgcct gcaaacagat cgggaccgcc ctggccacgt tgcgcagaaa
     2281 gcagatcgtg gtgatccgca gcagcgtgct gccctccatt ttcgacgaat gccgacgcat
     2341 cttcgcgtcg aacgcgcccg gactggtcga gctgtgcgcc aatcccgagt tcttgcggga
     2401 aggcacggcg attcttgatt tcgaggagcc acctttcacg atcctgggga cggacaccgc
     2461 ggccgcagag gacatgctgc gctcgctcta tgccgatcag acagctccca tctttgtgct
     2521 caagccccgg gaggcgctca tggtgaagta cgccagcaac gcctttcacg ccctgaaggt
     2581 ggcctttgcc aacgaaatcg cggccgtctg ccagccggcg ggagtcgatg ccgatgctgt
     2641 gatgaacatc ttctgcagcg acaccaaact caacatctcg cgtcgttatc tcaagccggg
     2701 attcgccttc ggcgggtcgt gcttgcccaa ggatgtgcga gccatcctct atgccggcaa
     2761 gggcatggac ctgcagcttc cgctgatggc agccattctg tcgagcaacg aagaggtcat
     2821 cgaacgcgcc ttccagaagg ttcgggccac gggcaagcgg cgcatcggca tgatc
//
LOCUS       DASPUL010000555         4547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_655008, whole genome shotgun sequence.
ACCESSION   DASPUL010000555 DASPUL010000000
VERSION     DASPUL010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655008"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VEJ00_12935"
     CDS             <1..295
                     /locus_tag="VEJ00_12935"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HXX02114.1"
                     /translation="AKMAKMHWIPKKQEAYPGQPEVAGAQHDVDFMVKDSKRFADSGG
                     WGYAEFEYDAASQTFRPGTVDDQPPQENDAKCGFGCHTIVKNRDYVFTEYAHR"
     gene            350..886
                     /locus_tag="VEJ00_12940"
     CDS             350..886
                     /locus_tag="VEJ00_12940"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HXX02115.1"
                     /translation="MKHRTALTVGTITVLLAILGSVAISAQDKYSLKTPSGIAFSDFK
                     GYENWVVVSSAMTPDDRLKVMVANPTMIEAYRAGVPGNGQPFPDGSKIAKLQWKFKKS
                     TEAPFVVNVPDTFAEAFLMEKDSKRFPDSGGWGYALFNYDPATDTFAPDAAPANCGHA
                     CHVAVKSKDYIFHPYQKR"
     gene            907..1554
                     /locus_tag="VEJ00_12945"
     CDS             907..1554
                     /locus_tag="VEJ00_12945"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXX02116.1"
                     /translation="MADLKLEVVVIPVADVDRAKRFYGSLGWRLDADFAFDNGFRVMQ
                     FTSPGSGCSVQFGTKMTSAAPGSAQGLYLIVSDIKAARDELVARGVEATEVFHPGAPG
                     AQFQPGAASGRVSGPAPDHASYSSFATFRDPDGNGWLLQEIRTRLPGRGLSLDVATLT
                     ELLREAEKRHGEYEPSAPKHHWSGWYAAYIIAREQGRVADDAAKDAALHMEGARR"
     gene            1551..2147
                     /locus_tag="VEJ00_12950"
     CDS             1551..2147
                     /locus_tag="VEJ00_12950"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXX02117.1"
                     /translation="MRADIVPGAVFPDYELSDHRGKHRKLSELQQNDPLVLVLSRGGF
                     CPKERRQHEGLVQLHREMQVGYCRLVTVSTDNLLETNEFRTGVGAHWPFLSDPGRKVQ
                     KDLDIAEYTDPHHNPMIPHTLVLEPGLRVYKIYNGYWFFRRPTVEELRQDLRAVLERC
                     RADWDISSAEQRAAWAKGDKAGFFPYGKTQAQLLADDD"
     gene            2169..3173
                     /locus_tag="VEJ00_12955"
     CDS             2169..3173
                     /locus_tag="VEJ00_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HXX02118.1"
                     /translation="MKCPLTKVQWIVSFGLLTVCWGWTASRADAQEEKSLAQQIFETM
                     IQLPGNNPHYRTAHAKGIVCEGTFTSSGKAAGLSKAAHFRAGSVPVIVRFSDASEDPY
                     LPDNSGVPRGMAIRFSLPDGGVTDIVSLSHNGFVVGTPDDFLALQKAAVATDPSKPHP
                     WPIEAFLGSRPLALKFIQEVQAVPASFGTQAFFSNDAFIFVSSQGVKQAGRYKFLPVA
                     GLQNLNEADAKAKPPNFLMDELKTRLARGPIQYRVIVQLPNAGDPTNDPSLVWPEDRK
                     TADVGLLSITSVVPDSDTAQKTLVFFPTNLTDGIELSDDPLPALRTSAYALSFARRQR
                     "
     gene            3190..4503
                     /locus_tag="VEJ00_12960"
     CDS             3190..4503
                     /locus_tag="VEJ00_12960"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HXX02119.1"
                     /translation="MTAHNSTTALLLSRWVLFLNGVAIAALAVLCPVTAAQGGGADAI
                     RPFKVQVPQQALDDLRRRVNATRWPDRETVTDQSQGVQLERLQALVRYWGSDYDWRQI
                     EAKLNALPQFVTTIDGIDIYFIHVRSKQPNALPIIITHGWPGSIIEQLKIIDPLANPT
                     AHGGRAEDAFDVVIPSIPGYGFSGKPTTTGWDPDRIARAWAELMKRLGYTHYVAQGGD
                     WGSPISSAMARQSAAGLQGIHINLPATLPPEVGAALATGAPAPAGLTEKEAAVFDALR
                     KYGMSGSSAYFTMLTARPQTVGYGATDSPAGLAAWILVHPGFAQWTYGADPNQSPTKD
                     DVLDNITLYWLTNTATSAGRLYWENGARGSVIAAVPQKTSEISLPVAITVFPDDVYRA
                     PETWARRAYRNLIYYHEVDRGGHFAAWEQPELFSAELRAAFRSLR"
BASE COUNT          910 a         1418 c         1424 g          795 t
ORIGIN      
        1 cgccaagatg gcgaagatgc attggatccc gaaaaagcag gaggcgtacc ccggccagcc
       61 cgaggtagcg ggcgctcagc atgacgtcga cttcatggtc aaggacagca agcggttcgc
      121 ggacagcggc gggtggggat atgccgagtt cgaatatgac gccgcgtcgc aaacgttcag
      181 gccgggaacc gtggacgatc agccgccgca ggagaacgac gccaagtgtg ggtttggctg
      241 ccacacgata gtgaagaata gagattacgt tttcacggag tacgctcacc gatgaatcgg
      301 tcgcgagtcg tgacaatgaa aggcgtcgaa ctttgtagag gggctggcga tgaagcatag
      361 aactgcgctg acggtgggga ccattacggt attgctggct atcttgggga gcgtggccat
      421 ttccgcacag gacaagtact cgctgaagac gccgagcggc atcgcgttct ccgacttcaa
      481 gggatatgag aactgggtgg tggtctcctc cgcgatgact cccgacgacc gcctgaaggt
      541 catggtcgcg aatccgacga tgattgaggc ctaccgggcc ggtgttcccg gcaacggcca
      601 gcccttcccc gatggctcca agattgcgaa gctgcagtgg aagttcaaga agagcacgga
      661 agcgcccttc gtcgtgaacg tcccggacac cttcgcggag gcctttttga tggaaaagga
      721 cagcaaaagg tttccggaca gcggcggctg gggctatgcg cttttcaact atgaccccgc
      781 gaccgacacc ttcgcgcccg atgcggcccc cgccaactgc gggcacgcgt gccacgtggc
      841 ggtgaagagc aaggactaca tcttccatcc ataccaaaaa cggtgagcga tctgcaggga
      901 tcgaccatgg ccgacttgaa gctggaggtc gtcgtgattc cggtggccga cgtcgatcga
      961 gccaagcgat tttacggaag cctgggatgg aggctagacg ccgatttcgc cttcgacaac
     1021 ggcttcaggg tcatgcagtt cacgtcgcca ggttcagggt gctcagtcca attcggcacg
     1081 aagatgacat cggccgcgcc tggctctgcc caggggctgt acctgatcgt ctcggatatc
     1141 aaagcggcgc gcgacgagct cgtcgcccgc ggcgtcgagg cgaccgaggt gttccaccct
     1201 ggcgcgcccg gagctcaatt ccagcccggt gccgcgagtg gccgagtcag cggaccggcg
     1261 cccgatcacg ccagctacag ctcattcgcc acattcagag acccggacgg caacggttgg
     1321 ctgctccagg agatcagaac gcggctaccc gggcgcggac tcagcctgga tgtcgcgacg
     1381 ctcacagagc ttctgcgaga ggcagagaag cgacacggcg agtacgaacc aagcgcacca
     1441 aagcatcact ggtcggggtg gtacgccgcc tacatcatcg cacgcgagca aggcagggtg
     1501 gccgacgatg cagccaagga tgctgcgctc catatggaag gcgcccgcag atgagggctg
     1561 acatcgttcc gggcgcagtg tttccagact atgagctttc ggaccaccgc ggcaagcacc
     1621 ggaagctctc ggagttgcaa cagaatgatc cgctcgtgct ggtgctctcg cgcggagggt
     1681 tctgtccaaa agagcgcagg caacacgagg ggctggtcca gctccatcgt gagatgcagg
     1741 tgggttactg ccgcctggtg accgtctcga ccgacaatct cctcgagacg aacgagttcc
     1801 gcaccggcgt cggtgcgcac tggccgtttc tctcggaccc agggcgcaaa gtgcagaagg
     1861 atctcgacat cgcggaatac accgacccgc accacaatcc gatgatccct cacacgctgg
     1921 tgctcgagcc cggcctgcgc gtctacaaga tctacaacgg ctactggttc ttccgccgac
     1981 ccacggtaga ggagctgcgc caggatctgc gtgcggtgct ggagcgctgc cgcgccgatt
     2041 gggacatcag cagtgcggag cagcgcgcgg cttgggcgaa gggcgacaaa gccggcttct
     2101 ttccctacgg aaaaacgcaa gcacaactgc ttgccgacga cgactgagcg gctcgaagga
     2161 gattgaccgt gaaatgcccc cttacgaagg tgcagtggat cgtcagtttc ggattactga
     2221 ctgtgtgctg gggctggacc gcttcccggg ccgacgccca ggaggagaag agtctcgccc
     2281 agcaaatctt tgagaccatg attcaactgc ccggaaacaa cccgcactac cggactgcgc
     2341 acgcaaaagg gatcgtatgc gaaggaacgt tcacatcctc agggaaagcg gcaggcctct
     2401 cgaaggcggc tcatttccgg gccgggtcgg tcccggtaat cgtgcgcttc tcggacgcgt
     2461 cggaggaccc ctacctgccc gacaactccg gcgtcccgcg cggaatggcc attcgtttca
     2521 gtctcccgga cggcggggtg accgacatcg tctctctgtc gcacaacggg ttcgtcgttg
     2581 ggacccctga cgactttctc gccctgcaaa aggccgccgt ggcgaccgat ccgagcaagc
     2641 ctcatccgtg gccgatcgag gcgttcctcg gctcacgtcc ccttgcgttg aagttcatac
     2701 aggaagtcca ggcagtacct gccagcttcg gcacgcaggc cttcttctcc aacgatgcgt
     2761 tcatctttgt cagcagccag ggagtgaagc aggcgggacg ctacaagttc ctccccgtcg
     2821 ccgggctcca gaatctgaac gaggcggacg ccaaagccaa gccgcccaat tttctgatgg
     2881 acgagctgaa gacgcgtctg gcgagagggc cgattcagta tcgcgtgatc gtccagcttc
     2941 cgaacgctgg tgatcccacg aacgatcctt ccctggtttg gccggaagat cgcaagaccg
     3001 ctgatgtcgg cctgctcagc atcacgtccg tggtacccga cagcgacacc gctcagaaga
     3061 cgctggtgtt ctttccgacc aacctgacag acgggatcga gctgtccgac gatccgttac
     3121 ccgctcttcg caccagtgcg tacgcgctgt cgttcgcacg gcgccagcgg tgaccgctag
     3181 gagtgcatcg tgactgcaca caactcaacg acggcgttgc tcctatcgcg ctgggttctg
     3241 ttcctcaacg gcgtggcaat agcagccctt gcggtgcttt gcccggtgac tgcagcgcag
     3301 ggcggtggcg cagacgctat ccgccccttc aaggttcaag tccctcagca agcactcgac
     3361 gaccttcgcc ggcgcgtcaa tgccacccgc tggcccgacc gcgaaacggt aaccgatcaa
     3421 tcgcaaggcg tgcagctgga aaggctgcaa gcgctcgttc gctactgggg cagcgactac
     3481 gactggcgac agatcgaggc caaattgaat gcccttcccc agttcgtcac tacgatcgat
     3541 ggaatcgaca tctatttcat tcatgttcgc tcaaaacagc caaacgcgtt gcccatcatc
     3601 atcacgcacg gctggcccgg ctcgatcatc gagcagctga agatcataga tccactcgcc
     3661 aatcccacgg cacatggagg acgcgcggag gacgccttcg acgtcgtcat cccatcgatc
     3721 ccgggctacg gattctcagg caaaccgacg accactggct gggatcccga tcgcatcgcg
     3781 cgcgcatggg cggagctgat gaaacgcctc ggatacaccc actacgtcgc gcagggtggc
     3841 gactggggct cgcccatctc gagcgcgatg gcgcgtcaga gtgcggcggg attgcaggga
     3901 atccacatca acttgccggc gacgctgccg cccgaggtgg gcgcggcgct cgcgaccggt
     3961 gcacccgcgc cggcggggct aaccgagaag gaagcggccg tgttcgacgc cctgaggaag
     4021 tacggtatgt ccggcagctc ggcctatttc acgatgctga ctgcgcgccc gcagacggtc
     4081 gggtacgggg cgacggactc accggccggc ctcgcggcct ggatcctcgt gcatccgggt
     4141 ttcgcgcagt ggacgtacgg cgccgacccg aaccagtcgc cgacgaagga cgacgtgctc
     4201 gacaacatca cgctgtactg gctgaccaac acggcgacat cggccgggcg gctgtactgg
     4261 gaaaacggcg ctcgaggcag cgtcattgcc gcggtcccgc agaagacctc cgagatctcg
     4321 ctgccggtgg ccatcacggt atttcccgac gatgtgtatc gcgctccgga gacctgggcc
     4381 cggcgcgcct atcgcaacct gatctactac cacgaggtcg ataggggcgg gcacttcgca
     4441 gcgtgggagc agccggagct tttcagcgcg gagcttcgcg ctgcctttag atcgcttcgg
     4501 taggcccaat gatctcggga gcggcgagtc tggccatttc acccgtg
//
LOCUS       DASPUL010000556         3737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2026429, whole genome shotgun sequence.
ACCESSION   DASPUL010000556 DASPUL010000000
VERSION     DASPUL010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2026429"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(331..2241)
                     /gene="ftsH"
                     /locus_tag="VEJ00_12965"
     CDS             complement(331..2241)
                     /gene="ftsH"
                     /locus_tag="VEJ00_12965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXX02120.1"
                     /translation="MNSTVKTVLFWLLIVVSAFLLWQVVKGARDSQKEKEINVTQFMS
                     DVDQGNIRELTVNGMEVKGKYRDGSSFHTTAPGNYFTPEMLKTLQSKGVNITFRDING
                     GNWAWLIQVVPIVLIGAVWIFMLRQMQTGGNKALSFGKSRARLLSMQQKKVTFKDVAG
                     VDEAKEELREIIEFLREAQKFQKLGGRIPKGVLLVGPPGTGKTLLARAVAGEANVPFF
                     SISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDERE
                     QTLNQLLVEMDGFESNEGVILMAATNRPDVLDPALLRPGRFDRRVVVSRPDVRGREEI
                     LRVHTRKIPLAEDVDLTVLARGTPGFSGADLANMVNEAALAAARQNRKAVLQYDFELA
                     KDKVLMGVERKSLLLSDEEKKNTAYHEAGHALVAAKLPHSDPVHKVTIIPRGMALGVT
                     MQLPVDDRHSLYKNYLETEIAILMGGRIAEEMFLNVMSTGAGNDIERATDTARKMVCE
                     WGMSDLGPLTFGKKEEQIFLGREIAQHRDYSEDTAIKIDQEVRKLVNNGYNTAKQILS
                     DNRDTLEKIARALIEREVLDANEIKMLVEGKELPPIQPPPSKSEDSVQHVIKPELQPG
                     RAPKTGERPATA"
     gene            complement(2395..2961)
                     /locus_tag="VEJ00_12970"
     CDS             complement(2395..2961)
                     /locus_tag="VEJ00_12970"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXX02121.1"
                     /translation="MNSPRVETLRAVITSEQIQKRVKELGRQISDDYKALKTGDEPIQ
                     VLAVLENAFIFVADLVRALEGPVVCQFIKPKYSRPKGASTDIMEILFTHEPDIRGQHI
                     LLVEGLVHSGVTSEFLMSDLRSRGAASVKLVTLLDRQSARRVSLQPDYFGFLVDETFL
                     VGYGLGAVEQTNRNLPYLAAANPRPPVT"
     gene            complement(3027..>3737)
                     /gene="tilS"
                     /locus_tag="VEJ00_12975"
     CDS             complement(3027..>3737)
                     /gene="tilS"
                     /locus_tag="VEJ00_12975"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXX02122.1"
                     /translation="EMGHPGTDRLQSKIDGAPWLVMNAAIDRLWFLGEPVAVQRRVVK
                     AVGEQAGIPLEFRHVEEILRFTTEEGPSGKELSLPLGWKVAREPEQIVFLTPGLREPR
                     QAGDYEYELRPQGTTAVSEIGIQIELRRMPADAAAKYESDLLLDPELLTFPLKVRNWR
                     PGDRFWPAHSKSPRKIKELLQERHVPQPERRLWPVVVSGDEIVWLRGFPVAAKSRGSR
                     GREAILILEQPLAKVVQR"
BASE COUNT          722 a         1213 c          973 g          829 t
ORIGIN      
        1 tgctgatccg gtctcgtttt gccctgaaaa tcggctggag atcgcgctcg gtccaactcg
       61 cttgaagccg ctcaagaagg acttcaagtt attgaaattc ctaatactta ggatcgattc
      121 cgactaacaa aattatacca caatacaagg ctctccggag gctcccgtca agcgtcgaat
      181 cgactcagta acaggcgaat tttcagccac tttgagagtt ctaattcgcc ccacgagcta
      241 accgtggtac ccggtccgcc gttccgattc cacgcaagag aaatgggcga caaagtcgcc
      301 catttcgttt tgcagagttg ttcggaggaa ttacgccgtt gccggccgct ccccggtctt
      361 cggggcacgc cctggctgca gttcgggctt gatcacgtgt tgtacgctgt cctccgattt
      421 ggacggcgga ggctgaatcg gaggaagctc ttttccttcg accagcattt tgatctcgtt
      481 cgcgtcgagc acttcccgct caatcaaggc tcgggcaatc ttctccagcg tgtcgcggtt
      541 gtccgaaaga atctgcttgg cggtgttata gccgttgttg accagcttgc gcacttcctg
      601 atcgatcttg atcgccgtgt cttcgctgta gtcgcgatgc tgcgcgattt cgcggcccag
      661 gaagatctgt tcttcctttt tgccaaacgt cagcggtccc agatcgctca tgccccattc
      721 gcagaccatc ttgcgcgccg tatcggtcgc acgctcaatg tcattgcctg cgcccgtgga
      781 catcacgttc aggaacatct cctcagcgat ccgaccgccc atcagaatgg ctatctcagt
      841 ttcaagatag ttcttgtaca ggctgtgccg gtcgtcgaca ggcagctgca tggtcacacc
      901 tagcgccatc ccgcggggaa taatcgtgac cttgtgcacg ggatccgaat ggggcaactt
      961 cgccgccacc agcgcgtgcc cggcttcgtg ataagccgtg ttcttctttt cttcgtcgga
     1021 gagcagcagc gacttgcgct ccactcccat caggactttg tccttggcca gttcgaagtc
     1081 gtactgcaga accgccttgc gattctgccg ggccgcggcc agcgcggctt cgttgaccat
     1141 attggcgagg tcggcgccgg agaatcccgg agtgcctcgc gccagcacag tcaaatcgac
     1201 atcctccgcc aaaggaatct tgcgcgtgtg cacgcgaagg atttcttcgc gtccccgcac
     1261 gtcaggacgg ctcacgacca ctcgccggtc aaaacgcccc ggtcgcagca gcgccgggtc
     1321 aagcacatcc ggccggttgg tcgccgccat caggatgacg ccttcgttcg actcaaagcc
     1381 gtccatctcg accagcagct gattcaaagt ctgctcgcgc tcgtcgtgtc cgccgcccag
     1441 gcctgcgccg cgatgccgcc ccacggcatc aatttcgtcg atgaaaatga tgcagggtgc
     1501 gttcttcttg ccctgttcaa acagatcgcg cacgcggctg gcgcctacgc ctacgaacat
     1561 ctccacaaag tcggagcctg aaatcgagaa gaagggaaca ttggcctcac cggcaacggc
     1621 gcgtgccagc aaagtcttgc ccgttcccgg aggcccaacc aacagcacgc cctttggaat
     1681 ccgcccgccc agcttctgga acttctgcgc ctcgcgcagg aactcgataa tctcgcgcag
     1741 ctcttctttg gcttcgtcga ctcctgccac gtctttgaac gtgactttct tctgctgcat
     1801 tgagagcagc cgggcccggc tcttgccgaa cgacagagct ttattcccgc cggtttgcat
     1861 ctgccgcagc atgaagatcc acaccgcgcc aatcaaaacg atcggaacga cctggatcag
     1921 ccacgcccag tttccgccgt tgatgtcccg gaacgtgatg ttgacgccct tgctctgcag
     1981 cgtcttcagc atttcgggcg tgaagtaatt gccaggggcc gtcgtatgga acgaggatcc
     2041 atcgcggtac ttgcctttca cttccatccc gttgacggtg agttcccgaa tattgccctg
     2101 gtcgacgtca ctcatgaact gggtgacgtt gatttctttc tctttctggc tgtcgcgggc
     2161 tcccttgacg acttgccaca gcaggaaggc cgatacaacg atcagcagcc agaacagcac
     2221 cgtttttaca gttgaattca cctaaaagac tcctaccttg gtcgagtgag gttacaccat
     2281 cgccccgatc cgctacagct attagatgcc cgacggcaac tttcgattgg cagattgccc
     2341 gagttcctat tctacctttt cccccaaaaa aaggcgcgtt cccttggccg taacttaagt
     2401 cactggaggc cttgggtttg cagcggccag atagggcaga ttccggttcg tctgctcaac
     2461 cgctcccagc ccatatccca ccagaaaagt ttcgtcgacc aggaagccga aataatccgg
     2521 ctgcagggac acccgccgcg ccgactgccg atccagcagc gttaccagct tcaccgatgc
     2581 cgctccccgg gaacgcaggt cgctcatcag aaattcgctg gtcacgccgg aatgcaccaa
     2641 cccttccacc agcagaatat gctgcccgcg gatgtccggt tcatgggtga acaggatttc
     2701 catgatgtcg gtggaggcgc ctttggggcg actgtatttg ggcttgatga actggcagac
     2761 gaccggcccc tcgagcgccc gaaccagatc ggccacgaag atgaatgcat tctcaagaac
     2821 cgccaatacc tgaatgggct catccccggt cttaagcgct ttatagtcgt cggagatttg
     2881 ccggcccagt tccttcaccc gcttctgaat ctgctcgctg gtaatcaccg cgcgcagcgt
     2941 ctcgactcgc ggactgttca tgagatctgc gtgcctccgg gcacgtcgcc cccaattcta
     3001 cgacgagcgc ctctcaacca ggtgatctac cgttggacga ccttggccaa tggttgttcc
     3061 agaatcagga tcgcctcgcg cccccgactg ccgcgggatt tcgctgctac gggaaagcct
     3121 cgcagccaca caatctcgtc gccactaacg accaccggcc acagcctgcg ctctggctgc
     3181 ggcacgtgac gctcctgcag gagctccttg attttccgcg gagacttcga atgcgcgggc
     3241 cagaaccgat ccccgggacg ccagttccga acctttagcg gaaacgtcaa caattccgga
     3301 tccagaagga gatccgactc atattttgcc gctgcgtctg ccggcattcg ccgcaactcg
     3361 atctggattc caatctcgct gaccgcggtt gtcccttgtg gtcgcagttc atattcgtag
     3421 tcgccggcct gtcgtggctc gcgcagcccc ggcgtcagaa acacgatttg ctccggctcg
     3481 cgcgcgactt tccatcccag tgggagtgaa agctccttgc cagaaggccc ctcttccgtc
     3541 gtgaatcgca aaatctcttc cacatgcctg aattcgagcg gaatccctgc ctgctccccg
     3601 accgccttca ccacccgccg ctgcaccgcg accggctctc ccagaaacca caagcgatcg
     3661 atagcggcat tcatcacgag ccacggcgcg ccgtcgatct tcgactgcag acggtcggtt
     3721 cccgggtgcc ccatttc
//
LOCUS       DASPUL010000557         4672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_655469, whole genome shotgun sequence.
ACCESSION   DASPUL010000557 DASPUL010000000
VERSION     DASPUL010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655469"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VEJ00_12980"
     CDS             <1..200
                     /locus_tag="VEJ00_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02123.1"
                     /translation="LVIEPDVGAFAYDDFKRSEDLIRVGEEAMRQALPEVRKWLEHPA
                     EAPAPAKDGRVVARPAPMPAD"
     gene            342..632
                     /locus_tag="VEJ00_12985"
     CDS             342..632
                     /locus_tag="VEJ00_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HXX02124.1"
                     /translation="MTRRQLDSKAAAETWQVQTAKARFSELFRRARTEGPQRITRQGK
                     EGVVMVAEEQYQRLVGRSQQPKGIVEFFRKSPLVGADLDLERVRDVGRDVEL"
     gene            629..1054
                     /locus_tag="VEJ00_12990"
     CDS             629..1054
                     /locus_tag="VEJ00_12990"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXX02125.1"
                     /translation="MSGFLLDTNCISELVRSQPEPRVLQWMEAADESLLYLSVLTLGE
                     IRKGVAGVPQGRRRTQLQTWLELDLQPRFSGRILPIDVPVADRWGLLAAEAKRKGKTL
                     ATIDGLLAATALHHNLTIVSRNVSDFASTPATILNPWEA"
     gene            complement(1093..1908)
                     /locus_tag="VEJ00_12995"
     CDS             complement(1093..1908)
                     /locus_tag="VEJ00_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02126.1"
                     /translation="MRPETKVQIARLSHTLLTAVVVLFLLGGLVLVLCAGLDINPFRE
                     TTTSLLVASFLGLIGLAAIVLILNVATNLSLIADARIEELKIQASGGVSRKWFVAFFA
                     VALALVGLVFGGTYLSKEKYLRDVRSQADDVLTSNQNLLDEISHRLVSGQLEDYRRIW
                     EIRNFLESQRSGLPSLNVIYSGRFEGKLAFYRVNDYYPVDSQKTYTPTYFACTKNLDC
                     EYLTNFFSGANVGVLQKYTLREDQFYIYIPFVGKEARFVLLFQRQNSYGKIGS"
     gene            complement(1943..3379)
                     /locus_tag="VEJ00_13000"
     CDS             complement(1943..3379)
                     /locus_tag="VEJ00_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HXX02127.1"
                     /translation="MSNENSFHMTPEEFRRYGYAVVDWIADYQARVESFPVLSQVKPG
                     EIRASLPGAPPAKGEPFDALLKDVEKLILPGVTHWQSPNFFAYFPCNASGPGILGDLL
                     SSGLGVQGMLWSTSPACTELETHVMDWLVGMLGLPEKFLSSKTGGGVIQDTASSAALC
                     ALLAGRERATHYASNKRGCDGRLVAYTSAHAHSSLEKAAMIAGIGRENLRLIEVDENF
                     AMRPEALAQRIEADKSTGLTPCFVCATVGTTSSNAMDPVAAIGEICRQRGLWLHVDAA
                     MSGTAMVCPEFRHLHNGVELADSYNFNPHKWMGANFDCSCFWVADRKELIQTLSVLPE
                     YLRNQATESGAVIDYRDWHIQLGRRFRSLKLWFVIRHYGVEGLQHHVRQHVKLAQQFA
                     EWVRKDDWFELAAPVPLNLVCFRHKGGDAVNQTIMDRLNRSGDLYLTHTKLNGKLTLR
                     MSIGQTHTEERHVERAWKRIQKEVSGSA"
     gene            complement(3504..4268)
                     /locus_tag="VEJ00_13005"
     CDS             complement(3504..4268)
                     /locus_tag="VEJ00_13005"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXX02128.1"
                     /translation="MPATNATSRFSDRVENYIRYRPGYPTQVFGVLEKDCGLRREHVI
                     ADIASGTGIWTRMLLEHGNAVFGIEPNSDMRQAGERLLADYSRFTSIAGTAEETALPA
                     ESVDFVTAAQAAHWFDREKARREFARILRPRGWLVLLWNERLTDATAFLRDYEQLLLD
                     YGTDYEQVRHERTTDAVKEFFDPLPYQERTFAMRQEFDYTGLEGRLLSSSYAPGPGHP
                     KHAPMLRELRRIFDAHVAGGRVAFEYTTRLYFGPLK"
     gene            4478..>4672
                     /locus_tag="VEJ00_13010"
     CDS             4478..>4672
                     /locus_tag="VEJ00_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02129.1"
                     /translation="MRVVIKVGTSLIAPGGQIDVNLLRGMVDQFDLSKNEYLIVTSGA
                     IAAGMSGLGLRERPKDVRGMQ"
BASE COUNT          978 a         1462 c         1277 g          955 t
ORIGIN      
        1 acctggttat cgaaccggat gtcggtgcat tcgcctacga cgacttcaag cgcagcgaag
       61 atctcatccg cgtcggcgaa gaagctatgc gccaggcctt gcccgaagtg cggaagtggc
      121 tggaacatcc tgcggaagcg cccgctcccg caaaagatgg ccgggttgtg gcgcgccccg
      181 cgcccatgcc ggcagactga accagtactc ctcttcctca agctttgttg ccggggttgc
      241 acttctagaa gtctgcagaa tccctgctca gaatcctctt gacaagagtc ggtcgattct
      301 ttctaataag actagtcaga ctagtcatat cgaggattgc catgaccagg cgccaacttg
      361 attcgaaagc cgcggcagaa acctggcaag tacaaacagc caaggcccgg tttagcgagc
      421 tcttccgacg cgctcgaacc gaggggccgc agcgcatcac aaggcagggc aaggaaggcg
      481 tcgtgatggt cgctgaggag caataccaac gtctggtcgg tcgatctcag cagccgaaag
      541 gcatcgtcga gtttttccgc aagtccccgc tcgtaggcgc agaccttgat ctggagcgcg
      601 tccgcgacgt ggggcgcgac gtcgagctat gagcggattc ctcctcgaca ccaattgcat
      661 ctccgagttg gtgcgcagcc aacccgaacc ccgcgttctg caatggatgg aagcagcgga
      721 cgaaagcctg ctttatctga gcgtactgac gctgggagag attcgaaaag gtgtggcagg
      781 agtgcctcag ggaaggcgcc ggactcaact gcagacctgg ctcgaactcg acctgcagcc
      841 tcgtttttcc ggaaggattc tgcccatcga tgtgcccgtt gccgaccgct ggggcttgct
      901 tgctgctgag gccaaacgca aagggaaaac gctcgctacc atcgacggcc tgctggctgc
      961 gactgcactg caccacaacc ttacgatcgt atcccggaac gtgtcggatt tcgccagcac
     1021 tccggcgacg attttgaatc cctgggaagc ttgaggcctt ctcgactcca gcatcctagc
     1081 gccaagattt ggtcaagatc cgattttccc gtagctgttc tgccgctgga acaacagtac
     1141 gaagcgcgct tctttcccga caaacggtat gtagatatag aactgatctt cgcgcaaagt
     1201 gtacttttgc agcacaccaa cattcgcacc agagaagaaa ttcgtaaggt actcacagtc
     1261 caggtttttt gtacaagcga agtaggttgg ggtgtaggtc ttctgcgaat cgaccgggta
     1321 gtaatcgttg acccgataga atgcaagctt gccttcgaac cttcccgagt aaatgacgtt
     1381 gagcgagggc aacccactgc gctggctctc aaggaaattc cggatttccc agatgcgcct
     1441 atagtcttcc agctggccag aaaccagccg atgactgatt tcgtccagca ggttctgatt
     1501 gctggttaga acgtcgtccg cctgcgaccg aacatcccgc aggtattttt ctttcgacag
     1561 gtatgtgccc ccaaacacca gaccgacgag ggccagagcg actgcaaaaa acgctacgaa
     1621 ccattttctt gaaacgccac cgctagcttg aatctttagt tcttcaattc tcgcatctgc
     1681 gatcaggctg agattggtgg caacgttcaa tatgagcacg atcgcagcca gcccgatcaa
     1741 cccaaggaag gaagcaacca gaagagaagt tgtagtttcg cgaaacggat tgatatccag
     1801 tccagcgcat agcacaagca ccaggccgcc gagaagaaag aggacaacta cggccgtcag
     1861 taacgtgtga cttagcctcg cgatttgcac ttttgtttcc ggtcgcatcg tctgagctcc
     1921 ttttcactgc ccgcaatgag ccctaggcgg atcctgacac ttctttttga attctcttcc
     1981 acgcccgctc cacatgccgc tcctcggtat gcgtctgccc gatgctcatc cgcagcgtca
     2041 gcttgccatt cagcttggta tgcgtgaggt aaagatcgcc gctgcggttc aacctgtcca
     2101 tgatcgtttg gttgaccgca tctccgccct tgtggcggaa gcacaccaga ttcagcggaa
     2161 ccggcgccgc cagctcgaac caatcatctt ttctcaccca ctcggcaaac tgctgcgcca
     2221 gcttcacgtg ctggcggaca tgatgctgca ggccttccac gccgtagtgc cggatgacaa
     2281 accacaactt cagcgaccga aagcgccgtc ccagctggat gtgccagtcg cggtagtcga
     2341 tcaccgcccc cgattccgtc gcctgattgc gcaaatactc cggcagcacg ctcagcgtct
     2401 ggatcagctc tttgcggtcg gccacccaga agcagctgca gtcgaaattc gcgcccatcc
     2461 acttgtgcgg attgaaattg tagctgtcag ccaattccac tccgttgtgc agatggcgaa
     2521 attccgggca taccatcgcc gtgcccgaca tcgctgcgtc cacgtgcagc caaagcccgc
     2581 gctgccggca gatttccccg atcgcggcga cgggatccat cgcgttcgac gatgtcgtgc
     2641 ccaccgtcgc gcacacgaaa cacggcgtga gcccggtgct cttgtcagcc tcgattctct
     2701 gcgcgagggc ttcggggcgc atggcgaagt tctcgtccac ttcgatcagt cgcagattct
     2761 cccgcccaat ccccgcgatc atcgccgcct tttccagtga cgagtgcgca tgcgccgacg
     2821 tgtaggcgac cagccgcccg tcgcatcccc gtttgttgct ggcatagtgc gtggcacgtt
     2881 cgcgccccgc aagcagagcg catagcgccg cgctcgaggc cgtgtcctga atgactcccc
     2941 cgccggtttt cgatgacagg aacttctccg gcagcccgag catcccaacc agccagtcca
     3001 tcacatgcgt ttccagttcc gtgcaggccg gactcgtcga ccacagcatc ccctgcacgc
     3061 cgagccccga cgaaagcaga tcccccagaa tccccggccc cgaagcgttg cagggaaagt
     3121 aggcgaagaa attcggcgac tgccagtgcg tgacgccagg caggatcagc ttctcaacgt
     3181 ctttcagcag cgcatcgaat ggctcgccct ttgccggcgg cgcgcctgga agcgaggccc
     3241 gaatctcccc cggcttcact tgcgagagca ccgggaacga ttccacgcga gcctgataat
     3301 cggcaatcca gtccacgacg gcgtagccgt agcgccggaa ttcctcggga gtcatgtgaa
     3361 atgaattctc gttggacatc gtgtgtctta gtgtagcggc aacctctcgg aactcacatg
     3421 gaatgcgtca tcccgaagcc ccgcgccctc accagcgggc gagggatctc gcgcgggccc
     3481 catcagagtg cgttgcacgg tctctatttc aacgggccaa aatatagccg agtcgtgtac
     3541 tcgaacgcca cgcgtccacc cgcgacgtgc gcatcgaaaa tgcgccgcaa ttcgcgcaac
     3601 atcggagcat gcttcggatg ccccggcccg ggcgcgtacg acgacgacag caaccgcccc
     3661 tccagcccgg tgtagtcgaa ttcctggcgc atggcgaaag tccgctcctg gtacggcaag
     3721 ggatcgaaga actcctttac cgcgtccgtc gtgcgttcat gccgcacctg ttcatagtcg
     3781 gtgccgtaat cgagcagtag ctgttcgtag tcgcgaagaa atgcggtcgc atctgtcaac
     3841 cgctcgttcc agagcagcac cagccatccc ctgggcctga gaatgcgcgc aaactcgcgt
     3901 ctcgctttct cccggtcgaa ccagtgcgcc gcctgagcgg cagtcacgaa atcgacgctc
     3961 tctgccggca aagcagtttc ttccgccgtc cccgcaatac tggtaaaccg cgaatagtca
     4021 gccagcagcc tctctcccgc ctgccgcatg tccgagttcg gctcgatgcc aaacaccgcg
     4081 tttccgtgct cgagcagcat gcgcgtccag atccccgtac ctgaggcaat atcggcgatc
     4141 acgtgctcgc gccgcaggcc gcagtctttt tcaagtactc caaaaacctg cgttggatac
     4201 cccggccgat agcgaatgta gttctccacg cgatcggaga agcggcttgt ggcgttcgtg
     4261 gctggcataa gcgatccctg ccggtttcga tctggcaagc acgggaaggt cgtcttcgcg
     4321 accttcgcgg cagtactttg agatctttgc gataagtctt ttctcgccgc gaagttcgcg
     4381 aagaacggcc gcaaagaacg caaaggaatc agagtcccct atcttccttt gtcttgccag
     4441 aaaacaattc taacgtcagg atagagttac tgtcccgatg cgtgttgtga tcaaagtcgg
     4501 caccagtctc attgcccccg gcgggcagat tgacgtcaac ctgctgcgcg gcatggtcga
     4561 tcagttcgat ctctcgaaga acgaatacct gatcgtgacc tcgggtgcca tcgcggcggg
     4621 aatgtcgggc ctgggtctgc gcgagcgccc gaaagacgtc cgcggcatgc ag
//
LOCUS       DASPUL010000558         3828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_939485, whole genome shotgun sequence.
ACCESSION   DASPUL010000558 DASPUL010000000
VERSION     DASPUL010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939485"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VEJ00_13015"
     CDS             <1..433
                     /locus_tag="VEJ00_13015"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HXX02130.1"
                     /translation="KEAGVVAAITPPVDVPEFFATRGRVPVRGTINSFPFRSSLMPMG
                     GCHMMPVNKALCQGAGAQPGDTVDVVMERDAEERTVEAPPGLKKELAKNKKAQERWEE
                     LAFTHKKEMANSISGAKQQETRKRRLAKVMQVLKTGAKWTG"
     gene            complement(524..1213)
                     /locus_tag="VEJ00_13020"
     CDS             complement(524..1213)
                     /locus_tag="VEJ00_13020"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXX02131.1"
                     /translation="MAVRPLLYYITDRTGFPGDEAARRRRLLEKIAEAARAGVDYIQL
                     REKDLSARELEHLARKAVGAVRQARAQASGNRPVTTALLINSRTDVALAVDADGVHLR
                     SDDVSPKDIREIWRRPGSGAQTREKPESRPLIGVSCHTPEEVAEAAAQGASFAVFAPV
                     FEKKGAPGAQAAGLAQLRKACQAKIPVLALGGVTLSNARSCLDAGAAGVAGIRLFQEN
                     DLEHVVRLLRG"
     gene            complement(1232..2110)
                     /locus_tag="VEJ00_13025"
     CDS             complement(1232..2110)
                     /locus_tag="VEJ00_13025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HXX02132.1"
                     /translation="MTDKLKAAPVAYQNEPFLNSPDGRILRMLAEYQEPLARFRREQI
                     QDTVVFFGSARFHGEKDAAKNLSAIESNHAGMPAAQLEKELKLAQAGIGMARYYEDAR
                     RLAYMLTKWSIQIPARRRRFVVTTGGGPGIMEAANLGAHEAGGKTIGLNIQLPFEQYP
                     NPYITPSLNFQFHYFFMRKFWFAYLAKALVIFPGGFGTMDELFEILTLAQTDKLAKKI
                     LVVIYGSEYWNRIINFQAFADAGAISPEDLSQFKMVDTPNEAFEYLRDGLTAYHLGGT
                     PKKAGEALPEIAKTRP"
     gene            complement(2141..2503)
                     /locus_tag="VEJ00_13030"
     CDS             complement(2141..2503)
                     /locus_tag="VEJ00_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02133.1"
                     /translation="MTDPTPLSSVPGKAANAPPATGTAVLDPPEQPTAPPEPSSVQLW
                     MHRTSVFLFVLISAVAGVLLIILPWTPGWTDNYLLLQFPGLRTVVSNGFFRGLCSGLG
                     LLDIWIGFWEALHYHEQH"
     gene            complement(2625..>3828)
                     /locus_tag="VEJ00_13035"
     CDS             complement(2625..>3828)
                     /locus_tag="VEJ00_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX02134.1"
                     /translation="AAASEPVVEELTADDIPSADVTVEVEEVAEETAPAAAEMHVAVE
                     PPAPPAEELPVTPEPVVAEAESKPGVLKEFVTDLESSLGSDFLPGTVAKPAAQPAAVP
                     AAKKRVEPPAPAPQAEPVVAAAPGAPVLGEFVADIEATLGEDFLKAAPVAEPASVAEA
                     APAAPFAKAKPAPPAKPAAPAVRKISAPLAASAAASASASAQPTPAVHAATAAPQVAW
                     PPVGEVHHPPVAMPAAHAANAPAAKTSPFGEDAGVDLAEMFGELKQDLESGVTAADED
                     PETHYNLGIAFREMGLLDEAIGELQKACQAFDHGHPFPQILQTYTWLAQCFLEKGVPE
                     AAVRWYSQALGVPTIDGETRVALNYELASAYETAGDKPTALKHFMEVYGRNIDYRDVA
                     ERIKALKS"
BASE COUNT          720 a         1211 c         1166 g          731 t
ORIGIN      
        1 caaagaggcg ggcgtcgtcg ccgccattac tccacccgtc gacgtgccgg aatttttcgc
       61 aacccgcggc cgcgtaccag tccgcggcac gatcaatagc tttcccttcc gatcctcgct
      121 gatgccaatg ggtggctgcc acatgatgcc cgtcaacaaa gctctctgcc agggcgccgg
      181 ggcgcagccc ggagacaccg tcgacgtcgt catggagcgt gacgcagaag aacgcaccgt
      241 cgaggccccg ccgggcctga agaaagaact ggcgaagaac aagaaagcac aagaacgctg
      301 ggaagaactg gcgttcaccc acaagaagga aatggcgaac tcgatcagcg gggccaaaca
      361 gcaggaaacg agaaagcgcc gcctcgcgaa ggtgatgcag gtcttgaaga ccggcgccaa
      421 gtggacggga taagaaccac tgggtagcgg ccagtagtca gtggtcagat cgtggactca
      481 tggcctttgc cggccactga tcactgacca ctgaccactg atctcatccg cgcaacaggc
      541 gcacgacatg ctccaagtca ttctcctgga aaagacgaat ccccgcgacg cccgcggctc
      601 cggcgtcgag gcaggagcga gcgttggaaa gagtaactcc accgagggct agaaccggga
      661 tcttggcttg gcacgctttt cgcagctgcg cgaggccggc agcctgcgct ccaggagcgc
      721 cttttttttc aaacaccgga gcaaacacgg caaagctcgc gccctgcgcc gcggcttctg
      781 ccacttcctc gggagtgtga caggaaaccc cgatcaacgg acgcgactct ggtttttccc
      841 gagtctgggc gcccgaacca ggtcttctcc agatttccct gatgtccttt ggcgagacgt
      901 catccgagcg caggtgaact ccgtcagcgt caacggccag cgccacatcc gtgcgcgaat
      961 tgatgagaag agcagtagtc actggccggt tgccgctggc ctgtgctcgt gcttgccgaa
     1021 cggcgccgac ggccttgcgc gcgagatgtt cgagttccct ggctgacagg tctttttccc
     1081 gtagctggat gtagtccact ccggcgcgcg ccgcttcagc aattttttcg agcagccgac
     1141 gccggcgggc agcttcatct ccaggaaatc cggtgcggtc ggtgatgtag tagagaagcg
     1201 gtcggacggc catcaattcc gggattgtag cctacggcct tgtcttggcg atctccggca
     1261 gcgcctcgcc tgccttcttc ggcgtgccgc ccaggtgata tgcggtcagc ccatcccgca
     1321 gatactcgaa ggcctcgttc ggcgtgtcta ccatcttgaa ctgactcaga tcttccggag
     1381 agattgcccc cgcgtccgca aacgcctgga agttgataat ccggttccag tactcgctcc
     1441 cgtaaatgac cacgaggatc ttcttggcca gcttgtcagt ctgcgccagc gtgaggattt
     1501 caaacagctc atccatcgtg ccgaacccgc cgggaaagat caccagtgcc ttggccaggt
     1561 aggcgaacca gaacttgcgc atgaagaagt aatgaaactg aaaatttagc gacggggtga
     1621 tatagggatt gggatactgc tcgaacggca gctgaatatt cagcccgatc gtcttgcctc
     1681 cggcttcgtg agcgcccagg ttggcggcct ccatgatgcc gggccctccg ccggtcgtca
     1741 ccacgaaacg ccgccgccgc gccggaatct ggatcgacca cttcgtcagc atgtaagcca
     1801 gcctgcgggc atcttcgtag taccgcgcca taccgatgcc ggcctgggcc agcttcagtt
     1861 ccttctcgag ttgtgccgcc ggcattccgg cgtgattgct ttcgatcgcc gaaaggttct
     1921 tggcggcgtc cttctctcca tgaaaccgcg ccgaaccgaa aaacaccacc gtatcctgga
     1981 tctgctcccg gcggaatcgc gccagcggct cctgatactc cgccagcatc cgcagaatgc
     2041 gtccatcggg actgttgaga aacggttcgt tctggtaagc gacgggcgcg gctttcagct
     2101 tgtctgtcat aactttctca gtcgggtcta ttcgggaaaa ctagtgttgc tcgtggtaat
     2161 gcagcgcctc ccagaagccg atccatatat cgagcagtcc cagcccgctg cacaggcccc
     2221 ggaaaaaccc gttcgaaacc accgttctca gtccgggaaa ctgcagcagc aggtagttgt
     2281 cggtccatcc gggtgtccag ggaagaatga tcagcagcac tcctgctacg gcgctgatca
     2341 agacgaaaag aaagacggaa gtccggtgca tccagagctg cacactgctg ggctccggcg
     2401 gcgccgtggg ctgctcggga ggatccagaa cggcggtgcc cgtcgccggg ggcgcatttg
     2461 ccgctttgcc cggcacgctg gagagcggag taggatcggt catgccttca gactccgccc
     2521 ttcccgccct tgcgcatcct tggtgagaaa gtgcgatggc ccgaccgtgc ccaacacaag
     2581 gttccgggcg ggagagaatt gaggacagaa cccatcgggc tattctacga cttcagggcc
     2641 ttgatgcgct cggcgacgtc gcgatagtcg atgttcctgc cgtagacttc catgaagtgc
     2701 ttcaacgccg tgggtttgtc gcccgcggtt tcgtacgcgg aggccagttc gtaattcagc
     2761 gcgacgcgcg tctcgccgtc gatcgtgggc acgccgagcg cctggctgta ccagcgcaca
     2821 gccgcctcgg gcactccctt ttccaggaaa cattgcgcca gccaggtgta ggtctgcagg
     2881 atctgcggga acggatgacc gtgatcgaac gcctggcagg ccttctgcag ttctccgata
     2941 gcttcgtcga gcaatcccat ctcgcggaag gcaatgccga ggttgtagtg tgtctcggga
     3001 tcttcgtctg ccgctgtgac tcccgattcc aggtcctgct tgagctcgcc gaacatttcg
     3061 gcaagatcga ctccggcgtc ctcgccgaaa ggagacgttt tggcggcagg cgcgttcgcc
     3121 gcgtgcgctg ccggcatggc aacgggtgga tggtgcactt cgcccacggg cggccaggct
     3181 acctgtggtg ccgcggtggc cgcgtgaacc gccggcgtgg gctgagccga agccgatgct
     3241 gacgccgcag cactcgccgc cagcggcgcg ctgatcttgc gaactgccgg cgcggcaggt
     3301 ttggcgggcg gtgcgggctt ggcctttgcg aacggagccg caggcgccgc ttccgccact
     3361 gaagcaggct cggccaccgg cgcagccttc aggaagtcct cgcccagagt tgcttcgata
     3421 tcggccacga actcgcccag aaccggtgcg cccggtgctg ccgcaacaac tggctcggct
     3481 tgaggagcgg gagctggcgg ctcgactctc ttcttcgccg ccggcacggc cgcgggctgt
     3541 gccgcaggct tggcaacggt gccaggcagg aagtcgcttc ccaaggacga ttccagatct
     3601 gtaacaaatt ccttcagcac gcccggcttt gactcggctt cggcaacaac gggttcgggc
     3661 gttaccggca gttcctcagc cggcggagca ggaggctcga ccgcaacgtg catctccgct
     3721 gccgcgggag ctgtttcctc cgccacctct tctacttcta cggtcacatc cgccgaagga
     3781 atatcatctg cggtcagctc ttccaccacg ggttcggatg cagccgca
//
LOCUS       DASPUL010000559         3487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_939583, whole genome shotgun sequence.
ACCESSION   DASPUL010000559 DASPUL010000000
VERSION     DASPUL010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939583"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VEJ00_13040"
     CDS             complement(<1..262)
                     /locus_tag="VEJ00_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02135.1"
                     /translation="MKCAKWIVGLLVTIMPLLATAQLQDKREIWAQVPFKFMVGAKTI
                     PAGECVIKRAPTESKTLLISNTTASVGLFSPVFPAETKTASRD"
     gene            445..900
                     /locus_tag="VEJ00_13045"
     CDS             445..900
                     /locus_tag="VEJ00_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXX02136.1"
                     /translation="MADKKVQFAEFELDFARFELSRNGARLRMESLPLQLLMFLVDNQ
                     RQLVTREQIADALWGKDVFVDVEQGINTAIRKIRIALGDDSGEPQYLQTIVGRGYRFV
                     AEINASNSASSEAGAGFSISAEEFGQAIMAAAGLAPEARKIPAKAQGES"
     gene            1046..2014
                     /locus_tag="VEJ00_13050"
     CDS             1046..2014
                     /locus_tag="VEJ00_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HXX02137.1"
                     /translation="MKAIQVKQPGGPEAMELAEVPVPQPKDNEAVVKLSASGVNYIDV
                     YYREGRYKASLPFILGQEGAGVVTAVGAEVKSPKPGDRVAWTGLLGSYAEYAAVPADR
                     LVPIPAGVTDRQAAAAMLQGMTAHYLSHDTYPLKRGETALVHAAAGGVGLLLVQMAHN
                     IGARVIATVSTEEKAKLAGEAGADDVILYTQTDFEAETRRLTAGKGVDVVYDSVGKTT
                     FEKGLNILRPRGMMVLFGGSSGPVPPFDLIALSQKGSLYVTRPTLAHYIASRDELVAR
                     SGAVFGMMASGKLKLRIEHTYPLAEAQRAHRDLEGRKTTGKLLLIP"
     gene            2045..3013
                     /locus_tag="VEJ00_13055"
     CDS             2045..3013
                     /locus_tag="VEJ00_13055"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HXX02138.1"
                     /translation="MKAIRVERTGGPEVMELVDLPVPHPKSGEALIKVAAAGVNSIDG
                     HFRDGSFRTPLPFIPGQEGSGVVTAVGSQVKAVKEGDRVAWSGALGSYAEYVASPEEH
                     LVPVPASITNEQAAAAMMHGLTAHYLVNDAHKLKAGETALVHAAAGGVGFLLVQLAHA
                     VGARVIGTASSDEKALLAREAGADEVIVYTREDFESEVKRLTAGKGVEVVYDGVGKAT
                     FEKNLNVMKLRGMLVLYGMSSGPVPPVDPAKLSEKGSLYMARTTLAHFTATREELLAR
                     TSDLFSMIAAGKLKLRIAKTYPLAEAPQAHRDLEARKMAGKLLLIP"
     gene            complement(3029..>3487)
                     /locus_tag="VEJ00_13060"
     CDS             complement(3029..>3487)
                     /locus_tag="VEJ00_13060"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HXX02139.1"
                     /translation="CGGILALLCGPFQFWTGLRNRALNIHRWTGILYLVGVAVGASGA
                     GMMAYFSTPRNFGVSLGFMALAWVLCTSVAYLAVLRRIIPLHREWMVRSYLVAFGFVT
                     FRVISDYPPGSHWGTFEDRAATVTWACWVVPMLGYEIVRAARRLLAVKTA"
BASE COUNT          736 a         1033 c         1095 g          623 t
ORIGIN      
        1 agtcgcggct ggccgtcttc gtttccgcgg gaaacaccgg agaaaacagg cccaccgacg
       61 cggtcgtatt actgatcaac agcgtcttcg actcggtagg cgcccgcttg atcacgcatt
      121 caccggcggg aatcgtcttg gcgccgacca tgaacttgaa gggcacctgc gcccaaatct
      181 cacgtttgtc ctgcagctga gcggtcgcca gcagcggcat gatggtaacc agcaacccca
      241 caatccactt tgcgcatttc ataagccacc tccgaaaggt ttttattccg tcgagcaagt
      301 gatggcagat tgcacccgat tcggaaaaag gtccatggag ccgttctgaa gcggtatcaa
      361 ttgatctgaa tattcataag ggcaccttga cactgccgcg gggacggaca aaaatgactg
      421 ctatcgaagg ggttctgggt tcccatggca gacaagaagg tccagttcgc cgaattcgaa
      481 ttggatttcg cgcgctttga gctctcccgc aacggagccc gcttgcggat ggagagtctt
      541 cctttgcagc tcctgatgtt cctggtcgac aaccagcgtc aactggtgac gcgcgaacaa
      601 attgccgacg cgctgtgggg gaaggatgtt ttcgtcgacg tagaacaggg gatcaacacc
      661 gccatccgca aaattcgcat tgccttggga gacgactccg gcgagccgca gtatctgcag
      721 actatagtcg gccggggata tcgcttcgtc gccgagatca atgcgtcgaa tagcgcgagt
      781 tcggaagccg gcgcgggctt ctccatttcc gccgaggagt ttggccaggc catcatggca
      841 gcggcaggcc tggcgccgga agcccgcaag atacccgcga aagcccaggg cgaatcttga
      901 ccgaattatc tctctagccc aacccagcgt tcacgactat cgaacgagcg gcaaacccgg
      961 cggccaggct cgtagttcag tgcctgcgca tgtagaatcg cctgtcacag aatcctggtg
     1021 agaagctcgc gaatccaaga ttgccatgaa agccattcaa gtcaagcagc cgggcgggcc
     1081 cgaggccatg gaactggcgg aagtaccggt tccgcagccg aaggacaatg aagcggtcgt
     1141 gaagctgtcc gcgtccggcg tgaattacat cgacgtctac tatcgcgagg gacgctacaa
     1201 ggcttccctt cccttcattc tcggacagga aggcgccgga gtggtcactg ccgtcggcgc
     1261 ggaagtgaag tcgccgaagc cgggcgaccg cgtggcctgg accgggctgc tgggctctta
     1321 tgcggagtac gctgcggttc cggctgaccg gttggtgccg atccctgccg gagtcaccga
     1381 tcgccaggcc gcggcggcca tgctgcaagg catgacggca cactacctgt cgcatgacac
     1441 atacccgctg aagcgcggcg agaccgcgct tgtgcacgcc gctgccggcg gcgtggggct
     1501 gctgctcgtc cagatggcgc acaacatcgg ggcgcgcgtg atcgctaccg tttcgaccga
     1561 agaaaaagcc aagctggcag gcgaagccgg agccgacgac gtcattctct atacgcaaac
     1621 cgattttgaa gcagaaacca ggcgcctgac cgcgggcaag ggcgtggacg tagtctatga
     1681 ctccgtgggc aagacgacct ttgaaaaagg actgaacatt ctgcggcctc gcgggatgat
     1741 ggttctgttc ggcggctcga gcgggcctgt gccgccgttc gatctgatcg cgctatcgca
     1801 gaagggatcg ctctacgtaa cccggcccac gctggcgcac tacatcgcct cgcgggatga
     1861 actcgtcgcg cggtcaggcg cggtgtttgg catgatggcc tcgggcaagc tcaagctgcg
     1921 catcgagcac acttatcccc tggccgaggc gcaaagggcg catcgcgacc tggaaggacg
     1981 gaaaacgaca ggaaaattgt tgctgattcc gtagctctta gttcttgtca tcctgagcga
     2041 actcatgaaa gccattcgcg tcgagcgcac cggcggtccc gaggtgatgg aactggttga
     2101 cctgccggtt ccgcatccca aatcggggga ggcgctgatc aaggttgccg ctgcgggagt
     2161 gaactcgatc gacggccatt tccgcgatgg aagttttcgc acgccgctgc cgttcattcc
     2221 cggccaggag ggctcgggcg ttgttaccgc tgtcgggtcg caagtgaaag cagtcaaaga
     2281 aggcgaccgg gtggcatgga gcggtgcgtt gggatcgtac gccgagtacg tcgcgtcgcc
     2341 cgaggagcac ctcgtgcctg ttccggcgtc catcaccaat gagcaggccg ccgctgcaat
     2401 gatgcatggt ctgaccgcgc actacctggt taacgacgcc cacaaactca aggcaggcga
     2461 gaccgcgctg gtgcatgccg cggccggcgg agtgggattc ctgttggtgc agttagccca
     2521 cgccgtgggg gcgcgagtca tcggcacagc atcgagcgat gagaaagcgc ttctggcgcg
     2581 cgaagccggg gcggatgagg tcatcgttta cacccgggag gacttcgagt ccgaagttaa
     2641 gcggctgacc gcaggcaaag gcgtcgaagt ggtctacgac ggggtgggca aagcgacgtt
     2701 tgagaagaat ctgaatgtca tgaagctgcg gggcatgctt gtcctttatg gcatgtcgag
     2761 cggaccggtg ccgcccgtgg atccggcaaa gctgtcggaa aaaggatcac tttacatggc
     2821 tcgcaccacg ctcgcgcact tcaccgccac gcgcgaggaa ttgctcgcgc gcaccagcga
     2881 tctgttcagc atgatcgccg caggcaagct gaagctgcgg atcgccaaga cctatccgct
     2941 cgcagaggca cctcaggcgc accgcgacct cgaagcgcgg aagatggcag ggaagctgct
     3001 gctgatcccg tagttgcgat tgattcctct aggcagtctt aaccgccagc agacgtcgcg
     3061 cggcacgcac gatctcgtat cccaacatcg gcacaaccca gcacgcccag gtcacggtgg
     3121 cagcgcggtc ttcgaatgtg ccccaatgcg atccgggcgg gtagtcgctg atcacgcgga
     3181 aagtcacgaa accaaacgcg accaggtagg agcgcaccat ccactcccta tgcagaggga
     3241 taatgcggcg aagaaccgcc agataggcca cgctggtgca gagaacccac gccagcgcca
     3301 tgaagcccag tgagacgccg aagttgcggg gtgtgctgaa gtacgccatc atgccggcgc
     3361 cgctcgcgcc cacagcgact ccaaccaagt agagaatgcc ggtccagcga tggatgttga
     3421 gagcgcgatt tctgagcccg gtccagaact gaaatggccc gcagaggagc gccagaatgc
     3481 ctccgca
//
LOCUS       DASPUL010000560         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1059102, whole genome shotgun sequence.
ACCESSION   DASPUL010000560 DASPUL010000000
VERSION     DASPUL010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059102"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1945
                     /locus_tag="VEJ00_13065"
     CDS             59..1945
                     /locus_tag="VEJ00_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXX02140.1"
                     /translation="MSTRVTLAVVSFVVLIVHGIVFYDQFFNRWEKHQTAYFEQARSL
                     AKTDAERRALDERSPRIEQIIVTQFGESRVDRCTTCHIASDDPRFQGHAEPLRTHPYS
                     AALGDVQRDGRWERRHKFTDFGCTICHDGQGRGLETFYAHGEDIFWPDPMLGYVTQAS
                     WHSQYKPKLTGKEYMQANCAQCHTEENFAGTPLVQRGRQLFFEKACYGCHRIEGLSTG
                     TLGPDLSEVGKKYKINYLWEHTVDPRAFQATSFMPKFDLTDDEIKAITVFLKSRRGIN
                     FAETSLGQYRARLQQTKLEESAKPQSPAAPSPVRGEQLVSERSCTACHKLGERDGHIA
                     PDLSYEGLIRDEDWMMAHFHEPKSRIPDSIMPVFGFADADFHSITAYLKTRSTPPSFK
                     TGEEIYKGLCARCHGEKGDGKGVTYLYLDPAPRDLTKAAFMNSKPEDRFLQSLKSGVP
                     GTSMPPWERVLTEEQRREVLAYVFQTFVKEPRPELKVRKVPDQNPVASNADSIHRGEE
                     IFLQRCTGCHGRKADGKGPNSLDISPRPRNLRNSFFVNSVPDHRLFDSILYGVQGTAM
                     PSWIDYGLSQNDVGDLINFIRSMNSRTGSTRGEKSGTLTSQASQSPMNQEVPCLNKGK
                     DNCR"
     gene            1912..>2104
                     /locus_tag="VEJ00_13070"
     CDS             1912..>2104
                     /locus_tag="VEJ00_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02141.1"
                     /translation="MPEQGQRQLQVTRTTPIGAVPLADSIHEDTTAKWFLLSAISYFF
                     VVGIIALTIAAKFVWPQLLG"
BASE COUNT          447 a          656 c          604 g          397 t
ORIGIN      
        1 ctgggagttc gtttcgccct ggtgaggaaa aagtcgaccg gaacgtgagg agaactttgt
       61 gagtacgcgc gtaacgctgg ctgtcgtgag ttttgtggtg ctgatcgtgc acggcatcgt
      121 tttttacgat cagttcttca atcgctggga gaagcatcag accgcctact tcgagcaggc
      181 ccgcagtctg gccaagaccg acgccgaacg gcgcgcgctc gacgagcgca gcccgcgcat
      241 cgagcagatc atcgtcacgc aattcggcga atcccgcgtc gaccgttgca ctacctgcca
      301 catcgccagc gacgatcccc gctttcaggg gcatgccgag ccgctgcgga cgcaccctta
      361 ttcggcagcg ctgggcgacg tgcagcgcga cggccgctgg gagcgccggc acaagttcac
      421 cgacttcggt tgcaccattt gccacgacgg acaaggccgc gggctcgaga ccttctacgc
      481 gcacggggaa gacatcttct ggcccgaccc catgctgggt tacgtgaccc aggccagctg
      541 gcactcccaa tacaagccga aactcaccgg caaggaatac atgcaggcga actgtgctca
      601 gtgccacacc gaagagaact tcgccggcac accgttggtg cagcgcggaa ggcagctgtt
      661 cttcgaaaaa gcctgctacg gctgccaccg cattgagggc ctctccaccg gaaccttggg
      721 gcccgatctc agcgaagtcg gcaagaaata caagatcaac tatttgtggg agcacacggt
      781 cgatccccgc gcgttccagg cgacttcgtt catgccgaag tttgatctca ccgacgacga
      841 gatcaaggcg atcactgtct ttctgaagag ccgtcgcgga atcaatttcg ccgagacttc
      901 cctcggccag tatcgtgccc ggctgcagca gacgaagctc gaagagtcgg ccaagccgca
      961 gagccccgct gcgccttcgc cggttcgcgg cgaacaactc gtctcggaac gttcctgcac
     1021 cgcctgccac aagctgggcg agcgtgacgg ccacatcgcc ccggatcttt cttacgaagg
     1081 actgatccgc gatgaagact ggatgatggc ccacttccac gagccgaagt cgcgcattcc
     1141 cgactcgatc atgccggtgt ttggttttgc cgacgccgac ttccactcca ttaccgccta
     1201 tctgaagacg cgcagcacgc cgccttcgtt caagaccgga gaagaaatct acaaggggct
     1261 ttgtgcccgt tgccacggtg aaaaagggga cggcaagggc gtgacttacc tctatctcga
     1321 tcccgccccg cgcgacctga ccaaagccgc cttcatgaac agcaagccag aagaccgctt
     1381 cctgcagtca ctgaagagtg gggtgccggg aacgtcgatg ccgccgtggg agcgcgttct
     1441 gaccgaagag cagcgccgcg aggtcctggc ttacgttttc cagacgtttg tgaaagagcc
     1501 acgtcccgaa ctcaaggtgc gcaaggtgcc ggatcagaat ccggttgcgt caaatgccga
     1561 ctcgattcac cgcggagaag aaatcttcct gcagcgttgc accggttgcc acgggcgcaa
     1621 ggccgacggc aaggggccga actcgctcga catcagtccg cggccgcgca acctgcgcaa
     1681 ctcttttttt gtgaatagcg tgcccgacca caggctcttc gattccatcc tgtatggcgt
     1741 gcaaggcacg gccatgcctt catggatcga ctacggcctg agtcagaacg acgtgggcga
     1801 tctcatcaac ttcattcgca gtatgaattc tcggacaggc tcaactcgcg gcgagaaatc
     1861 cggaacgctc acctcccagg cgagccagtc cccaatgaac caggaggtgc catgcctgaa
     1921 caagggcaaa gacaattgca ggtaacgcgc acgacaccga tcggcgcagt tccactggct
     1981 gattcgatcc acgaagacac gacggcaaag tggttcttgc tgagcgccat ctcttacttc
     2041 tttgttgtgg gaatcattgc tttgaccatc gctgccaagt tcgtctggcc gcagttgttg
     2101 ggaa
//
LOCUS       DASPUL010000561         3777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_104565, whole genome shotgun sequence.
ACCESSION   DASPUL010000561 DASPUL010000000
VERSION     DASPUL010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104565"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1024
                     /locus_tag="VEJ00_13075"
     CDS             38..1024
                     /locus_tag="VEJ00_13075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXX02142.1"
                     /translation="MDRREFLKNAAATTVVTTLGVKGMLGAADSFPYRTLGRSGEKVS
                     LIGLGGYHIGKQSDEQESIRIIRTALDNGVNFLDNCWDYNGGVSEIRMGKALRDGYRQ
                     KAFLMTKIDGQTKKAAEQQLEESLRRLQTDHVDLLQFHEVIRETDPARIFGEGGGMEA
                     ALAASKAGKIRYIGFTGHKSPDIHLKMLETGFAHQFTFDAVQMPLNVMDTHYDSFEKK
                     VLPVLVKHEIGVLGMKPMGDAIILRSKTASAVECLHYAMNLPTSVVITGCDSLPILQQ
                     ALDAARTFKPLSKEEVKQLLAKTESAATKGEYELYKTTHNFDGTFMNPQWLG"
     gene            complement(1047..2762)
                     /locus_tag="VEJ00_13080"
     CDS             complement(1047..2762)
                     /locus_tag="VEJ00_13080"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HXX02143.1"
                     /translation="MKTLRSQAWFGRKDRDGFLYRSWMKNQGWPHDLFDGRPVIGICN
                     TWSELTPCNAHFRDLAEFVKRGVWQAGGFPLEFPVMSLGETQLRPTAMLFRNLASMDV
                     EESIRGNPIDGVVLLMGCDKTTPSLLMGAASCDLPTIGLSGGPMLSGKFRGHDLGSGT
                     GLWQMTDQVRRGEMTKEEFFEAESCMHRSKGHCMTMGTASTMASMVEALGMSLPGNAA
                     IPAVDARRNTLAQMTGRRIVQMVHEELRMSQILTRGAFENAIRANAAIGGSTNAVIHL
                     IALAGRVGVPLCLEDFDRLGSSLPCLLNIQPSGAHLMEDFFYAGGLPRVLRELGEAGV
                     LNRDALTANGKSIWDNVAAAPCWNRDVIHAFNAPFKAEAGIAVLRGNLAPDGAVIKPS
                     AASPRLLQHRGRAVVFETIEEYHARIDDETLDVDENCVLVLKNCGPRGYPGMAEVGNM
                     MLPAKVLRKGVTDMVRISDARMSGTAYGTVVLHACPEAAAGGPIALVQPGDMITLDVP
                     QRLLRLEVDDATLAARRAKWSSPPVPARGWVKLYVEHVQQADKGADLDFLVGHSGAPV
                     ARDSH"
     gene            2927..>3777
                     /locus_tag="VEJ00_13085"
     CDS             2927..>3777
                     /locus_tag="VEJ00_13085"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXX02144.1"
                     /translation="MSRVQPSPLNRSGFLGVGSFLMVVLSAALLASTTMWAERDFQKV
                     LQVFFVDVEGGQATLFVTPAHQSLLIDTGWPGNDGRDADRIVAAAKKAGISEIDYVLI
                     THFHNDHVGGLPQLVARIPVETVIDHGDNRESTDAPTVQVWQAYQQLLTENKFKRLTV
                     KPGDKLPVKGVQATVISSDGAVIDHALAGAGQENAVCKGGESYAADQTENRRSLGTLI
                     TFGKLRILDLGDLTRDKEMELVCPNNKLGAMDIYIVSHHGWYQSGSPAFLNAIAPRVA
                     IMDNGAK"
BASE COUNT          811 a         1137 c         1110 g          719 t
ORIGIN      
        1 cgtctcgacc gagcgccggg agccaaggag cgactctatg gatcgaagag aattcctgaa
       61 gaatgctgcg gccacaacgg tcgtaacaac tcttggcgtc aaaggcatgt tgggcgcagc
      121 ggattcgttc ccttaccgca cgcttggccg ctcgggcgag aaagtatcgc tcatcgggct
      181 cgggggctac cacatcggca agcagtcgga cgagcaggaa agcatccgca tcattcgcac
      241 cgcgctcgac aacggcgtca actttctcga caactgctgg gactataacg gcggagtcag
      301 tgaaatccgc atgggcaagg cgctgcgcga cggctaccgg cagaaggcgt ttctcatgac
      361 caagatcgat gggcaaacga agaaagctgc cgagcagcaa ttggaggagt cactccgccg
      421 cttgcaaacc gatcacgttg atctcttgca gtttcatgag gtcatccgcg agaccgaccc
      481 cgctcgaatc tttggtgagg gcgggggcat ggaagcggcg ctggcagcca gcaaagccgg
      541 caagatccgc tacatcggct ttaccgggca caagagtccc gacatccatc tcaagatgct
      601 ggagaccggc ttcgctcacc agttcacctt cgatgccgtg cagatgccgc tgaacgtgat
      661 ggacacgcac tatgacagtt ttgaaaagaa agtgctgccg gtgctggtga agcacgagat
      721 cggcgtgcta ggcatgaagc ccatgggcga tgccattatt cttcgcagca agaccgcttc
      781 tgccgtggag tgcctgcact acgccatgaa tctgccgacc agcgtggtga tcaccggctg
      841 cgattccctg ccgatcttgc agcaggcgct cgatgcggct cgcaccttca agccgctgag
      901 caaggaagaa gtgaagcaat tgctcgccaa aaccgagtcc gcagccacca agggagagta
      961 cgaactgtac aagaccacgc acaacttcga cgggacattc atgaacccgc agtggctggg
     1021 gtagtgcgct gctcgcgttg ttgaggtcag tgcgagtcgc gcgcaactgg ggcgccgctg
     1081 tgaccgacca ggaaatcgag gtcggcacct ttgtccgcct gctgcacgtg ctcaacgtac
     1141 agcttcaccc aacctcttgc tggaacagga ggcgacgacc actttgcccg ccgggccgcc
     1201 aaggtcgcgt cgtcaacttc cagacgcaac aggcgctgcg gaacatcaag ggtaatcatg
     1261 tctccgggct gcaccagagc gatcggcccg cctgcggccg cctcggggca agcgtgcaga
     1321 acgaccgtgc cgtaagccgt gccgctcatg cgagcgtccg atatgcggac catatcggtc
     1381 acgcctttgc ggagaacttt tgccggcagc atcatgtttc ccacttccgc catcccggga
     1441 tagccgcggg gcccgcagtt cttcaacacg agaacgcaat tctcatcgac atcgagagtc
     1501 tcgtcgtcga tccgggcgtg atattcctcg atggtttcga atacgaccgc ccgcccgcga
     1561 tgctgcaaca ggcgtggact tgcggcggaa ggcttgatca cagctccatc cggcgccaga
     1621 ttgccacgca gaacggcgat tccggcttcg gctttgaatg gtgcgttaaa ggcatggatc
     1681 acgtcgcgat tccagcacgg cgcagccgcc acgttgtccc agatcgattt cccattcgca
     1741 gtcagtgcat cgcgattcaa gactccggct tcgccgagct cgcgcagaac tcgcggcagg
     1801 ccgccggcat agaagaaatc ctccataagg tgcgctcccg aaggttgaat gttcagcaga
     1861 cagggcaaag aggacccgag ccgatcgaaa tcctcaaggc agaggggcac gcccacacgg
     1921 ccggcgagtg caatcagatg aatgacagca ttggtcgatc cgccaatcgc tgcattcgcg
     1981 cggattgcgt tttcaaaggc tccgcgtgtg agaatttgcg acatgcgcag ttcttcgtgg
     2041 accatctgca caatgcgccg tcctgtcatc tgggcaagag tgttgcgacg agcatcgacc
     2101 gcaggaatgg cagcgttgcc cggcaagctc atgcccagtg cttcgaccat gcttgccatg
     2161 gtggaggccg tgcccatcgt catgcagtgc cccttggaac gatgcatgca actttcggcc
     2221 tcaaagaatt cttctttcgt catttcgcca cgccgcacct gatctgtcat ttgccacagg
     2281 cccgtgcccg agcccagatc gtgaccgcgg aatttgccgg agagcatcgg gccgccggaa
     2341 aggccaatcg taggcagatc gcaggacgcc gcacccatca gcaacgaagg agtcgttttg
     2401 tcgcagccca tcagcaggac gacgccatcg attggattcc cgcgaatcga ctcttccacg
     2461 tccatgctgg ccaggttgcg gaacaacatg gcagtcggac gcagttgggt ttcccccagg
     2521 gacatcaccg ggaactcgag gggaaagccg ccggcctgcc aaacgccgcg tttcacgaat
     2581 tcggccagat cgcggaaatg ggcgttacac ggcgtcagct cggaccaggt gttgcagatc
     2641 ccgatcacgg gccgcccatc gaacaggtcg tggggccagc cttgattctt catccagctc
     2701 cggtaaagaa agccgtcccg atccttccgc ccgaaccacg cctggctgcg taacgttttc
     2761 attggtcgtc ctcagtttgc gggagaggcg tcgtcgcacc tcttgccctg gccgatcata
     2821 gcgcaaagtc agtgggtaaa cgtaatctca cttcaggttt ccgcttgacg aatcgcacgg
     2881 ggtgaacgat gatctcgata cttctcatcc gcaggaaagg tctcacatgt ctcgcgttca
     2941 accgtcaccg ctgaatcgct ccggctttct cggcgtcggc tcattcttga tggtcgtgct
     3001 ttccgcagcg ctgctcgcgt ccacaacgat gtgggcggag cgcgattttc aaaaggtatt
     3061 gcaagtgttc ttcgttgatg tcgagggcgg gcaggccact ttgttcgtaa ccccggcgca
     3121 ccagtccctg ctgattgaca ccggatggcc gggaaatgac gggcgcgacg ccgaccgcat
     3181 tgttgccgca gcaaagaaag ctggcatcag cgagattgac tacgttctga tcactcactt
     3241 tcacaacgat cacgtcggcg gcctgccgca gctggtggcc cgcattccgg tcgaaacggt
     3301 catcgatcac ggcgacaatc gcgagagcac ggacgcgccc acggttcaag tttggcaggc
     3361 ctaccagcag ctactgacag agaacaagtt caagcgcctg acggtgaaac ccggggacaa
     3421 gctgcctgtt aagggagttc aggctacggt catcagctca gatggcgccg tgatcgatca
     3481 cgccctcgca ggtgcgggac aggaaaacgc cgtctgcaag ggaggcgagt cctatgcagc
     3541 ggatcagacc gagaatcgac gatcgctggg gaccctcatc acttttggca agctgagaat
     3601 tctggatctt ggcgatctga cgcgcgacaa agaaatggaa ctggtctgcc cgaacaacaa
     3661 gctgggcgcg atggatatct atatcgtttc gcatcacggc tggtatcaga gcggaagccc
     3721 tgcattcctg aacgcgatcg ctccgcgcgt cgcgatcatg gacaacggcg cgaaaaa
//
LOCUS       DASPUL010000562         8915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1880792, whole genome shotgun sequence.
ACCESSION   DASPUL010000562 DASPUL010000000
VERSION     DASPUL010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8915)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8915
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1880792"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /gene="hpnA"
                     /locus_tag="VEJ00_13090"
     CDS             <1..906
                     /gene="hpnA"
                     /locus_tag="VEJ00_13090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HXX02145.1"
                     /translation="RLLVRPTSNLKNLEGLKAETATGDLRDPASLEKAIQGCDTVFHV
                     AADYRLWVRDPDEMYRSNVEGTRAILDAARKNGVRRVVYTSSVATMGFTSNGSPADEE
                     SPVSLADMIGPYKRSKFMAEQIAMEAGRSGLHVVTVNPTTPVGEQDVKPTPTGRIVVD
                     FLKRKFPAYVDTGLNLVDARECARGHVSALERGRSGERYILGGENLTLKQILDKLEKI
                     TGLPSPKVKLPYVFAFAAGVVDEAITGRLLKRKPRATVDTVRMGKKKMYASSDKAERE
                     LGWKIVPVEDALRRAVEWFRANGYV"
     gene            899..1612
                     /locus_tag="VEJ00_13095"
     CDS             899..1612
                     /locus_tag="VEJ00_13095"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02146.1"
                     /translation="MSRIAIVAALEREIRPLVKGWRVSEKQHSGRNFRFYEQQGVVLV
                     CGGIGAEAARRAAEAVIVLFQPALVCSVGFAGALDPALNVGEIVRPHRVVNATDGSSV
                     TLEGGEGVLVSFSSVASPAQKAKLRESFAAQAVDMEAAAVARAAEARGVAFVAIKAIS
                     DDPDFVFPALERFVDSDGQFQEARFAWFAVIRPWLWPGLIRLARNGNRASRALCDRLQ
                     TMVAETLAREPASEVVERR"
     gene            1609..2148
                     /locus_tag="VEJ00_13100"
     CDS             1609..2148
                     /locus_tag="VEJ00_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02147.1"
                     /translation="MTRKIVLLLPVIVMFAVPVFAQSAPPTIYAESFRKGATHIIDDS
                     FDVKLSPANPAYRERIRDSHGADRYELTIAPHGPEGDNRITSWRVALRDLHHTIYSNV
                     LLEDQEPSQDPGNNLSWLNPDQFGAVPILARRIVKVDSFYVLFQVKALHFTPLDSPYL
                     DSMTVTFTLSNKDPRSAQP"
     gene            2250..2957
                     /locus_tag="VEJ00_13105"
     CDS             2250..2957
                     /locus_tag="VEJ00_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXX02148.1"
                     /translation="MHFFPLLAAFVGGEIVDMISNTGAVAKLVLLSLLAFSLVSWAII
                     LTKWSLLRRARAQSGRFVRAFRKAQRLQDIAAVAEQFKPSPLVGVFEGGFEEYKRQAS
                     SLGALRNLTAIQRGMQIGASEEITRLERNVPWLAITAAVTPFVGLFGTVWGIIDAFHG
                     LGTAGAATLRAVAPGISEALVTTAAGLAAAIPAVIAYNLIGASIREFAARSDDFALEV
                     LNAVEHSQAQVPAEARR"
     gene            2958..3386
                     /locus_tag="VEJ00_13110"
     CDS             2958..3386
                     /locus_tag="VEJ00_13110"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXX02149.1"
                     /translation="MAFTTSNGRTQSALADINITPLVDVVLVLLIIFMVTAPVLQSGI
                     DVSVPKTRTVREITQERLVITINKDQRVFLGNDAVNINEIGTKLRQRIRDPRNQFIFV
                     RADENVPFGAFATVMDAVKQSGITNVSIVTQPLEQNVGKR"
     gene            3370..4167
                     /locus_tag="VEJ00_13115"
     CDS             3370..4167
                     /locus_tag="VEJ00_13115"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXX02150.1"
                     /translation="MSASADIYAEHGRWGRALAWSAGLHVGITALLLLYSAFFYGTHG
                     EGWGAGGGGEAIGATLVSTVPLPANPAQTQNVLANESKGLTQSQPKIEEKAPDAIEIQ
                     GKNAKIKPKPKQKQETASKQKPQPAPEEETNQVAFGEGGPVSGPYGTFSADGAKGGFG
                     ITGGGGDFGTRYAWYVQVIQRKVADNWLKYEVDPRITSAQRVYITFDIARDGHPSNVQ
                     VEQSSGVPSLDISATRALQRIDTFGPLPTDYSGSKISVEFWFDYSRK"
     gene            4338..5711
                     /gene="tolB"
                     /locus_tag="VEJ00_13120"
     CDS             4338..5711
                     /gene="tolB"
                     /locus_tag="VEJ00_13120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HXX02151.1"
                     /translation="MEQAARPALQRHAKTLPLLVLAVLLAATVFPPALVAQDWVKTGT
                     GLGVEKVRLAVPDFKPSTADPQNAALLKTFNDTLWNDLDVSGVVELVSKSFYPLQVPG
                     QPPEVNFVAWNAPPPNAAMLAFGSLGVASGRLTVQGWLYDVKNSQSPQILGKQYTDAA
                     IDAAARVMAHKFADEIIFRLGGGIPGIAESQIVFVSDRTGHKEIWGMDYDGSNQHQIT
                     RLGAIALSPRISPDGSRVAFSALTKTGWEIMMYSLDLNRLVSFPHMGGTNLSPAWSGD
                     GTKLAFSSSRAGEPEIYVADVSGGNLHRMTTDKGPDVSPVWNRKTNAQIAFVSGRTGL
                     PQIYTMEADATNQQRLTDQGYAVSPNWSPNGQFLAFAWVRKYGPGEPGSSDIYLMDIA
                     SKQWVQLTHDGGRNDFPSWSPDGRHIIFQSNRSGSLEVWSMLADGTKVQQLTFTGRNT
                     QPNWSWK"
     gene            5769..6506
                     /gene="pal"
                     /locus_tag="VEJ00_13125"
     CDS             5769..6506
                     /gene="pal"
                     /locus_tag="VEJ00_13125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXX02152.1"
                     /translation="MKERHVKYFALVVMLGAVMMLGACKKKAAPPPPPPPPPPPAPTA
                     SISVSPASIQAGQSASLTWQTTNATDVSIDGIGAVQPNGSQSVSPTDSMTYHLVAKGS
                     GGTQEATTRLTVTQPPPPPPPPAPTVTDEDLFNQNIKDVYFDYDKSDIRADQRASIQA
                     DMMFLTQHPNMSFTIEGHCDERGSTEYNLALGDKRASAVKNELTTAGISASRIKTISY
                     GKEKPFCTESNEACWQQNRRGHFVYQK"
     gene            6712..7548
                     /locus_tag="VEJ00_13130"
     CDS             6712..7548
                     /locus_tag="VEJ00_13130"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX02153.1"
                     /translation="MKPHRICTLAVCSVMLLAIAPAWAVNKDMVQLQTQVQQLQEQMT
                     AMQRSFDERMGVMKNLVEQDTDAVNKVAAALTTLQTTLQKQQTDAGAKSDQLSGQIQA
                     LNDTLDELKVRLAKVSKQLEDLQASQQSIQAQQAQQQAQQQAQAQAPPPDVLYNNALR
                     DYNGNKNDLASQEFSDYLKFYPNTDLAGNSYFYLGEIQFRQGNYQQAAQSYDQVLQNF
                     PSGNKAASAQLKKGFALIELGKQDDGVSELRHLIQRYPHSPEALQARDRLRKLGVSST
                     GR"
     gene            complement(7571..7741)
                     /locus_tag="VEJ00_13135"
     CDS             complement(7571..7741)
                     /locus_tag="VEJ00_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02154.1"
                     /translation="MVRNISFNPYSVCIDPTNDLPRIVRSHEHNLSYVYTFTNWNVTS
                     VTLSKDIATLGK"
     gene            7890..8090
                     /locus_tag="VEJ00_13140"
     CDS             7890..8090
                     /locus_tag="VEJ00_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02155.1"
                     /translation="MLHGKSDQDLAKGAIEVEDHKIPGNIGLEKQLGHRDQDPMLKDA
                     DTDFPEPGFNPEHSGEPEVKSA"
     gene            complement(8120..>8915)
                     /locus_tag="VEJ00_13145"
     CDS             complement(8120..>8915)
                     /locus_tag="VEJ00_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02156.1"
                     /translation="GHNLFEKGMLMKRRHLVSAFGILLLAAGALFAQEKPGTVAALEF
                     QKPKNGMVPQYEAGRKQKAAWHKQQNDPLALYVWETLSGEDTGTFIVGRLGQHWADFD
                     KPAVSDEADIAEFQKVVGGYVDSVVTHYYEFLPKISNPTDGNTPSKFSEIVTFYIRYG
                     KGAEFRSALDRIHEAAQKTKWPPNYYWYTLASGGPSGVYVLVLPRNSWADFEEKPEVK
                     PFRDMIKDAFGQTEADSIIDRLDESVKEETTAIVKFRGDLSYLPGK"
BASE COUNT         1825 a         2730 c         2666 g         1694 t
ORIGIN      
        1 cgcctgctcg tccgccccac cagtaatctc aaaaatctcg agggactcaa agcggaaacc
       61 gcgactggcg acctccgcga cccggcctca ctcgaaaaag ccatccaagg ctgcgacaca
      121 gttttccacg ttgccgccga ctaccggctc tgggtgcgcg atcccgacga aatgtaccgc
      181 tccaacgtcg agggcacgcg ggctattctc gacgccgcgc gcaaaaacgg tgttcgccgc
      241 gtcgtctaca cctccagcgt tgcgaccatg ggtttcacca gcaacggctc tcccgctgac
      301 gaagagtctc ccgtctccct tgccgacatg atcggtccgt acaagcgctc gaaattcatg
      361 gcagagcaaa tcgccatgga agccgggcgc agcggcctgc acgtcgttac agtgaatccc
      421 acaactcccg taggcgagca ggatgtgaag ccaactccga ccggccgcat cgtcgtcgac
      481 ttcctcaagc gcaaatttcc ggcctacgtc gacaccgggc tcaatctggt cgacgcgcgg
      541 gagtgcgccc ggggccacgt atcagccttg gaacgaggca ggtccggcga acgttatatt
      601 ctgggcggag agaatctgac gctgaagcag attctcgaca agctggagaa gatcaccggc
      661 ctgccctcgc ccaaggtgaa actgccgtac gtgttcgctt ttgccgcagg tgtggtcgac
      721 gaagccatta cgggccgcct gctcaaacgc aaaccgcggg cgacggttga tacggttcgc
      781 atgggcaaga aaaaaatgta cgccagctcc gacaaggcgg agagggaact gggctggaag
      841 atcgtgccgg tcgaggatgc tttgcggcgc gccgtcgaat ggttccgcgc caacggatat
      901 gtctaggatc gcaattgtgg ctgcgctgga gcgcgagatt cgtccgctgg tgaagggctg
      961 gcgcgtcagc gagaagcagc acagcggccg gaactttcgc ttctatgagc agcaaggcgt
     1021 cgtgctcgtc tgcggtggaa tcggagcgga agctgcgcgc cgcgccgcag aggccgtgat
     1081 tgtgctgttc caacctgcct tggtgtgctc tgtgggcttt gccggcgctc ttgatccggc
     1141 attgaacgtg ggcgagatcg tgcggccgca tcgcgtggtc aacgcgaccg acggcagcag
     1201 cgtgactctg gaggggggcg aaggcgtgct ggtcagtttc tcatcggtag cgtcgccggc
     1261 gcagaaggcg aagttgcggg agtcgttcgc cgcgcaggca gtcgatatgg aggccgcagc
     1321 cgtagcccgc gccgctgagg ccagaggtgt ggcgttcgtg gccataaagg cgatttccga
     1381 tgacccagac ttcgtctttc ctgcccttga gcgcttcgtc gattcggatg gtcaatttca
     1441 agaggccagg ttcgcgtggt ttgccgtgat ccgtccctgg ctgtggccgg ggttgattcg
     1501 cctggcgcgc aacggcaacc gggcttcacg cgctctgtgc gatcgcttgc agacgatggt
     1561 cgcggaaacc ttagctcgcg agcctgcttc ggaggtggtt gaacggagat gacacgcaag
     1621 atcgttctcc tgctgccggt gatcgtgatg tttgccgtcc cggtgttcgc ccagagtgca
     1681 ccgcctacga tctatgcgga gagtttccgc aaaggcgcca cccatattat cgacgacagc
     1741 ttcgatgtca agctcagtcc ggccaatcct gcgtatcgcg agcgcatccg cgattcccac
     1801 ggcgccgatc gctatgaatt gacaatcgcg ccgcatggac cggagggcga caaccggatc
     1861 acctcctggc gtgtcgcgct gcgcgacctg catcacacca tctacagcaa cgttctgctc
     1921 gaagaccagg agccctcgca ggatcccggg aacaacctga gctggctcaa ccccgaccag
     1981 tttggggccg taccgatcct cgcccgccgc atcgtcaagg tcgacagttt ttacgtccta
     2041 tttcaagtga aggcgctgca cttcacgccg ctggattcgc catacctgga ctcgatgacc
     2101 gtgacgttca ccctaagtaa caaggacccc cgcagcgcgc aaccttgagg agccgaagat
     2161 ttctccgcgc cattgccgga tcggatacgt gcggaatagc tcccgcgctc gttcatctat
     2221 aatcgaacca ggcattctcc aaaaccccaa tgcatttttt ccccttgctg gcagcttttg
     2281 tcggcggcga aatcgtcgac atgatttcca acaccggagc ggtggccaag cttgtgctgc
     2341 tgtcactgct ggccttcagc ctggtttcct gggccatcat cctgaccaaa tggagcctgc
     2401 tgcggcgcgc gcgcgcgcag agcggccgtt tcgtgcgcgc ctttcgcaaa gcgcagcggc
     2461 tgcaggatat cgccgccgtc gccgaacagt tcaagccaag tcccctggtc ggcgtatttg
     2521 aaggagggtt tgaagagtac aagcggcagg cttcctcgct cggcgcgtta cggaacctca
     2581 cggcgatcca gcgcggcatg cagatcggcg cgtcggagga aatcacgcgc ctggagcgca
     2641 atgtgccctg gctggccatc accgccgcag tcacgccgtt tgtcgggctt tttggaacgg
     2701 tctggggcat catcgacgcg tttcacggac tgggcacggc cggtgccgcc acgttgcgcg
     2761 ccgtagcgcc cggaatttcc gaagctttgg tcaccacggc cgctggactt gccgccgcca
     2821 ttcccgccgt gattgcctac aacctgattg gcgcatcgat tcgtgagttc gccgctcgca
     2881 gcgacgattt tgctctcgaa gtgctcaatg ccgttgagca cagccaggcc caggttccgg
     2941 ctgaagcgag gcgctgaatg gcattcacca cctcgaacgg acgcacccag agcgctcttg
     3001 ccgacatcaa catcactccc ctcgtcgacg tagtgctggt tctgctgatc attttcatgg
     3061 taacggcgcc ggttctccag tcgggcatcg atgtgagcgt gcccaaaacg cgcaccgtgc
     3121 gcgaaatcac ccaggaaagg ctcgtcatca ccatcaacaa agaccagcgt gtttttctgg
     3181 gcaacgatgc ggtcaacatc aatgaaattg gaaccaagct gcggcagagg attcgcgatc
     3241 cgcgcaacca gttcattttc gtgcgcgccg atgagaacgt tcctttcggc gccttcgcta
     3301 ccgtaatgga cgcggtgaaa cagtcgggca ttaccaacgt gagcatcgtg acgcaaccgc
     3361 tggagcaaaa tgtcggcaag cgctgacatc tacgccgaac acggccgctg gggacgcgcc
     3421 ttggcctggt cggcgggcct gcacgtgggc attaccgcgc tcctgttgct gtactctgcg
     3481 tttttttatg gaacgcacgg ggaaggctgg ggcgccggcg gtggtggcga agcgatcggc
     3541 gctacgctgg tcagcaccgt tccactgccg gcaaatcctg cgcagacgca aaacgtgctc
     3601 gccaatgaat ccaagggcct gacgcagtcg cagcccaaga tcgaagagaa ggcaccagac
     3661 gcgatcgaga tccagggtaa gaacgcgaaa ataaagccca agccaaagca gaagcaggaa
     3721 accgcctcga agcagaagcc gcagcccgct cctgaggaag agacgaatca ggttgcgttt
     3781 ggcgagggcg gtccggtcag cgggccgtac ggtaccttca gcgccgacgg ggcgaagggc
     3841 ggcttcggca tcactggagg cggaggcgat ttcggcacca gatacgcctg gtatgtgcaa
     3901 gtgattcagc gcaaggtcgc cgacaactgg ctgaagtacg aggtcgatcc ccgaattacg
     3961 agcgcgcagc gggtttacat caccttcgac atcgcccgcg acgggcatcc ttcgaacgtg
     4021 caggtggagc agtccagcgg ggtgccttca ctcgacattt ccgcgacccg ggcgctgcag
     4081 cgaatcgata cctttggacc attgccaacc gactacagcg gcagcaaaat ttcggtcgag
     4141 ttctggttcg actacagcag gaagtgaacc ttggcagtaa tcatcgctgc actctcgatt
     4201 catcggggtg tggcgcgctg ggagcagaat aaggggtggg gttttcccga ggaacgcgga
     4261 acatggagac gggtttgtta agagattcat atcgtgatac gatattgtcg tgcggggtat
     4321 gcttctccgc aaggcggatg gaacaagcgg cacgaccagc gctgcagcgg cacgcgaaga
     4381 cgctcccgct tcttgttctg gcggttctcc tggcagcaac cgtcttcccg ccagccctcg
     4441 ttgcgcagga ttgggtcaag accggcaccg gcctcggcgt ggagaaagtc cgcctggctg
     4501 ttcctgactt caagccgtcc acggctgatc cgcaaaacgc cgctctgctc aagaccttca
     4561 acgacacctt gtggaacgac ctcgacgtct cgggcgtcgt cgaactcgtc tccaagagct
     4621 tctacccgct gcaggtgccg gggcagcctc ccgaggtcaa tttcgtcgcc tggaatgcgc
     4681 ctccgcccaa tgccgccatg ctggcttttg ggagtcttgg ggtggccagc ggccgcctga
     4741 ccgtccaggg ctggctctac gacgtgaaga atagccagtc gccgcagatt ctcggcaagc
     4801 agtacacaga cgctgcgatc gacgcggcgg cccgggtcat ggcccacaag ttcgccgatg
     4861 aaattatctt ccgcctgggg ggcggcattc ccggcatcgc cgaaagccag atcgttttcg
     4921 tcagcgaccg caccggtcac aaggaaatct ggggcatgga ttacgacggc tcgaaccagc
     4981 accagatcac gcgtctgggt gcaatcgcgt tgtcgccgcg aatctcgccc gacggctcgc
     5041 gcgtcgcatt cagcgctctg accaaaacgg gctgggaaat catgatgtac tcgctggatc
     5101 tgaaccggct ggtaagcttt ccacacatgg gcggcacgaa cctctcgccc gcctggtcag
     5161 gcgacgggac caagcttgcg ttttcctcct cgcgggcggg ggaaccagaa atctatgtag
     5221 ctgatgtctc gggcgggaat ctacatcgta tgacgacgga caaagggccg gacgtttctc
     5281 cggtatggaa tcgcaagacc aacgcccaga tcgcctttgt gagcggccgc accggccttc
     5341 cgcagattta caccatggaa gccgacgcca ccaaccagca gcgcctgacc gatcaaggct
     5401 acgcggtttc tcccaattgg tcgccgaacg gacagtttct ggccttcgcg tgggttcgca
     5461 aatatggtcc cggggaaccc ggatcgtccg acatttatct catggacatc gccagcaaac
     5521 agtgggtgca gctcacgcat gacggaggac gcaacgattt tccttcgtgg tcgccggatg
     5581 ggcggcacat catttttcag tcgaatcgct ccggcagtct ggaagtctgg agcatgctgg
     5641 ccgatgggac caaggtgcag caactgacgt tcacgggacg aaatacgcaa ccgaactgga
     5701 gttggaaata atcaaacgct tcgtccgacc ttgaagggca ggtcttcggg gtcgcaggga
     5761 ggaacaaagt gaaggaacga cacgtgaagt atttcgcgct cgtagtcatg ctgggcgctg
     5821 tgatgatgct gggcgcctgc aaaaagaaag cggctccgcc gccacccccc ccaccgccac
     5881 cgcctccggc cccgacggct tcgatctcgg tgagcccggc gtcgattcaa gcgggccagt
     5941 cggcctcgct gacctggcag acgaccaatg ccaccgatgt ctcaatcgac ggcattggcg
     6001 cggtgcagcc caacggatcg caatctgtgt cgccaaccga ctccatgacc taccatctgg
     6061 tcgccaaggg ctcgggcggg acgcaggaag ccaccacacg gctcaccgtg acccagccgc
     6121 caccgccgcc accgccgcca gctccgaccg tcacggatga ggacttgttc aatcagaaca
     6181 tcaaggatgt ctacttcgac tacgacaagt ccgatatccg ggccgaccag cgcgcgtcga
     6241 tccaggccga tatgatgttt ctgacgcagc atcccaacat gagcttcacc atcgagggac
     6301 actgcgacga gcgcggctca acggagtaca acctggcgct cggcgacaag cgcgccagtg
     6361 ctgtcaagaa cgagctgacc acggccggca tcagcgccag ccgcatcaag acgatcagct
     6421 acggcaagga aaaaccgttc tgcaccgaga gcaacgaagc ttgctggcag cagaaccgcc
     6481 gcggccactt cgtgtaccag aagtgaggtc gatgggggac gccggcgacg gtgccccccg
     6541 attctgctcc gaggtcgcgc gtacgtctgc gccgcgctgc aagctcgccc gaacgcgcct
     6601 gctcatgccg cggttgcgtc tgccggttcg ccgcggttcc tttctctgcg gaatcctccc
     6661 ggcctgtggg acaatcatgg catctaaacc aatgtattca gggaatcctc tatgaaacca
     6721 catcgtatct gtacgttggc cgtgtgttcc gtgatgttgc tggcaatcgc tccggcgtgg
     6781 gcggtgaaca aagacatggt gcagttgcag acccaggtgc agcaactgca ggagcagatg
     6841 actgccatgc agcgcagctt cgacgagcgc atgggcgtga tgaagaactt ggtcgagcag
     6901 gacaccgacg ccgtgaacaa ggtggccgcc gcgctgacca ctcttcagac cacgctgcag
     6961 aaacagcaga cggatgctgg cgccaagtcc gatcagctct ccggccagat tcaggcgctt
     7021 aatgacaccc tcgacgagct caaggtccgg ctggccaagg tgtccaagca actggaagac
     7081 ctgcaggcat cccagcaaag catccaggcg cagcaggccc agcaacaggc acagcagcag
     7141 gcccaggctc aggctccgcc cccggacgtg ctctacaaca acgctctgcg cgactacaac
     7201 ggcaacaaga acgacctggc gtcgcaggag ttctccgact acctcaagtt ttatcccaac
     7261 accgatctgg cggggaacag ctacttctac ctcggtgaaa ttcagttccg gcagggaaac
     7321 taccagcagg cggcgcagag ctacgaccag gtcttacaaa acttccccag cggcaacaag
     7381 gccgcctcgg ctcagctgaa gaagggcttc gccctgatcg aactgggcaa gcaggatgat
     7441 ggtgtcagcg agttgcgcca tctgatccag cgttatccgc actcgcccga agccctgcag
     7501 gcccgcgacc gtctgcgcaa gcttggggtt tcgagcacgg gaaggtaggg atcgggcaaa
     7561 agcccgggtg ctacttgccc aaagtcgcga tgtccttgga aagcgtgact gacgtcacgt
     7621 tccagttggt gaaagtgtag acgtaactca gattgtgttc gtgcgagcgg acgatgcggg
     7681 gaaggtcgtt cgtcgggtcg atgcacaccg agtaagggtt gaaggaaata ttccgcacca
     7741 cgggttcgct tccggccggg aacacgaccg ttcaggtctc actttataac tatttccttc
     7801 cgaaagcgcc aggcataaac ttggtgcagg cggggcggct ctcgtctctc gttaccgctt
     7861 gtcgccgatt catagcacga ggtggaatca tgctacacgg caagagcgat caggatctcg
     7921 ccaagggcgc gatcgaagtt gaggatcaca agattccggg gaacatcggc ctggagaagc
     7981 agctcggcca tcgcgaccag gacccgatgc tcaaagacgc cgacacagat tttcccgagc
     8041 cgggcttcaa ccccgagcac agcggggaac cggaagtgaa gagcgcataa aggcccgtgc
     8101 cggagcacgg gcctcggacc tatttgcccg gcaagtaact caaatcgccg cggaatttga
     8161 cgatggcggt tgtctcttcc ttcaccgact cgtccagacg atcgataatc gaatccgctt
     8221 cggtttgtcc gaacgcgtcc ttgatcatgt cgcggaacgg cttcacttcg ggtttctcct
     8281 cgaaatccgc ccagctgttg cgcggcagaa ccagcacata gactcctgac ggtccaccac
     8341 tggccagcgt gtaccagtag tagttggggg gccatttcgt cttctgcgcc gcctcatgga
     8401 tgcggtcgag agccgagcga aactctgcgc ccttcccgta acggatgtag aaggtcacga
     8461 tctcagagaa tttcgagggc gtgttcccgt cggtagggtt gctgatcttc ggcaggaatt
     8521 catagtagtg ggtgacgacc gaatcgacgt aaccgccgac cactttttga aattccgcga
     8581 tatcagcctc gtctgagacg gccggtttgt cgaagtcagc ccagtgctga ccgagccggc
     8641 ccacgatgaa agttcctgtg tcctcgccgc tgagcgtttc ccatacgtat agcgccagcg
     8701 gatcgttctg ctgcttgtgc catgcggctt tctgcttccg tcccgcctcg tactgcggca
     8761 ccatcccgtt ctttggtttc tggaattcga gagctgcaac ggtgccaggt ttctcctggg
     8821 caaacaacgc acctgctgct aatagaagga tcccaaaagc tgacactaaa tgcctgcgtt
     8881 tcatgagcat ccccttttca aacaagttgt gaccg
//
LOCUS       DASPUL010000563         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1202600, whole genome shotgun sequence.
ACCESSION   DASPUL010000563 DASPUL010000000
VERSION     DASPUL010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202600"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..1874)
                     /locus_tag="VEJ00_13150"
     CDS             complement(387..1874)
                     /locus_tag="VEJ00_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXX02157.1"
                     /translation="MAKPNILILWGDDIGWWNISFNNRGQMGYRTPNIDRVAEEGLSF
                     SDYYGQQSCTAGRAAFITGQNPIRTGLTKVGMPGADVGLMAEDPTIAELLKPLGYATG
                     QFGKNHLGDKDKFLPTMHGFDEFFGNLYHLNAEEEPEDPDYPKDAKFKELFGPRGVLH
                     SWADGRGGQKVEDTGALTKKRMETIDDEVTEHALRFIDDSVKAGKPFFVWYNTTGMHF
                     RTHSADKHRGKSNGQGEYNDVMVAHDEQIGRMLDKLDELGIADNTIVLYSTDNGPHYN
                     SWPDAGITPFRSEKNTNWEGGWRVPAFTRWPAKFKAGTVLNGVVSHQDWMTTLLAAAG
                     ASDIASKLRAGHKIGEKTYKVCLDGFNMLPYFTGEAQKSPREFFFYISDDGDILAIRM
                     GDWKVVLMEQRAKALACWFEPFVHLRAPKMFHLRRDPFERADESSNTYWDWLISHAYI
                     IYAMQAVVSNQIESFRAFPPRQKPASFNLDAVLRKLQEANSSANH"
     gene            complement(1995..>2548)
                     /locus_tag="VEJ00_13155"
     CDS             complement(1995..>2548)
                     /locus_tag="VEJ00_13155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HXX02158.1"
                     /translation="MFFDGVLAAYVYGESSLCVLRPACGEGVALEHTGDVYSCDHYVE
                     TGCLLGNILETPLRDLLLSDKQRAFGEAKSLALPSTCRECRWLFTCGGECPKNRVLVT
                     ADGEPGLNWLCEGLRAFFEHSEHPMRVMAALLQQARPAAEAMGVLAEEDRRRAALFAA
                     AGRNGPCPCGSGLKFKKCHGAQR"
BASE COUNT          424 a          838 c          787 g          499 t
ORIGIN      
        1 atagacccga aggttcccct tccgtcctcc cggccgatcc gccgcctgcc tctgccggcc
       61 tcacaaaaga tcggctgaaa ccaggcgata gccatgtttg atcattcggg gttgcatgcg
      121 ccatcaaccc cggccagtcc acatagccgg tttggcaagg ccgcgcaaga ggcgccggtg
      181 aagtttctgg ggtcggccgc tgatcggccg actcggattc gggccaccca tcccctcgcc
      241 gcctttgtga cgacgccgcc catcctgacg gctcgatgtt tacaacgcaa aaatatagtc
      301 ggccgctcat cgcgttgacg tcaaaccagc cccttgagtc ggcgaccagc gatccaccgg
      361 ccttcgcaaa cggtcttcca acaaactcaa tggttggcgc tgctgttggc ctcctgcaat
      421 ttccgcagaa ccgcgtcgag gttgaacgag gcgggcttct ggcgcggcgg gaacgccctg
      481 aatgactcga tctggttcga gacgaccgcc tgcatcgcat agatgatgta ggcgtgcgag
      541 atcagccagt cccagtaggt gttcgagctt tcgtcggcgc gctcgaacgg gtcgcgcctg
      601 aggtggaaca tcttcggcgc ccgcaggtgc acgaacggct cgaaccagca tgcgagcgcc
      661 ttcgcccgct gctccatgag cacgaccttc cagtcgccca tgcggatcgc gaggatgtcg
      721 ccgtcgtcgc tgatatagaa gaagaactcc ctcggactct tctgcgcctc tcccgtgaag
      781 tagggcagca tgttgaagcc gtcgaggcag accttgtagg tcttctcgcc gatcttgtgg
      841 cccgcccgca atttgctcgc gatatcggac gctcccgcag ccgcgagcag cgtcgtcatc
      901 cagtcctgat ggctgacgac gccgttgagc accgtgcccg ccttgaactt cgcgggccag
      961 cgggtgaaag ccggcacccg ccagccgccc tcccaattgg tgttcttctc cgaccggaac
     1021 ggcgtgatgc cggcgtccgg ccaggaattg tagtgcgggc cgttgtcggt cgagtagagg
     1081 acgatcgtat tgtcggcgat cccgagttcg tcgagcttgt cgagcatccg gccgatctgc
     1141 tcgtcatgcg caaccatgac gtcgttatat tcgccctggc cgttgctctt gccccggtgc
     1201 ttgtccgcgc tgtgggtgcg gaagtgcatg ccggtcgtgt tgtaccagac gaagaacggc
     1261 ttgccggcct tcaccgagtc gtcgatgaag cggagcgcgt gctccgtcac ctcgtcgtcg
     1321 atggtctcca tgcgcttctt ggtgagcgcc ccggtgtcct cgaccttttg tccccctctg
     1381 ccgtccgccc agctatgcag cacgccgcgc gggccgaaca gctccttaaa tttggcgtcc
     1441 ttcggatagt cggggtcctc cggctcctct tccgcgttga ggtgatagag gttgccgaag
     1501 aattcgtcga aaccgtgcat cgtcggcagg aacttgtcct tgtcgccgag atggttcttg
     1561 ccgaactgcc cggtcgcgta gccaagcggc ttcaagagct cagcaatggt cggatcctcc
     1621 gccatgaggc ccacatccgc gcccggcatg ccgaccttcg tcaagccagt gcggatcggg
     1681 ttctggccgg tgatgaacgc cgcccggccg gcggtgcagc tctgctggcc gtagtagtcg
     1741 ctgaacgaga gcccttcttc ggccacccgg tcgatgttgg gagtccggta gcccatctga
     1801 cctcggttgt tgaagctgat gttccaccag ccgatgtcat ccccccacag gatgaggatg
     1861 ttgggcttag ccatctctgc cacggaaacc tccttggatg tccctcatcc ggccgaacgc
     1921 cgggtgggct tcgactgcct ctcgcgatag tgatagcagt tgctggggac ggaagcaatc
     1981 tcaggacgcg gcactcatcg ctgcgcccca tggcacttct tgaacttcag gccgctgccg
     2041 caggggcagg ggccgttgcg gccggcggcc gcgaagagcg cggcgcgtcg ccggtcctct
     2101 tcggcaagga cgcccatggc ctcagcggcc ggccgcgctt gctgcagcag ggccgccatc
     2161 acacgcatgg gatgctcgct gtgctcgaag aaggcccgca acccctcgca gagccaattg
     2221 agtcccggct cgccgtcggc ggtgacgagc actcggttct tggggcattc ccctccacac
     2281 gtgaacagcc agcgacattc gcggcaggtg gacggcaggg cgagcgattt ggcttcaccg
     2341 aaggcacgct gcttgtccga cagcaacagg tcgcgaaggg gcgtctccag gatgttcccc
     2401 aacaggcagc ccgtttcgac atagtggtcg caggagtaca cgtccccggt gtgctcgagc
     2461 gccaccccct cgccgcatgc gggccggagc acgcataggc tggactcgcc gtagacgtag
     2521 gcggcgagga ccccgtcgaa gaacatga
//
LOCUS       DASPUL010000564         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_515800, whole genome shotgun sequence.
ACCESSION   DASPUL010000564 DASPUL010000000
VERSION     DASPUL010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515800"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VEJ00_13160"
     CDS             complement(<1..267)
                     /locus_tag="VEJ00_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02159.1"
                     /translation="MPQTIIEKIAQAHMADGPKRPLRAGDFLSVRPFHVMTHDNSSAV
                     MSKFKAIGAKKIYDPKQLVFALDHDIQNREEANLKKYRSIEAFAA"
     gene            complement(289..1767)
                     /locus_tag="VEJ00_13165"
     CDS             complement(289..1767)
                     /locus_tag="VEJ00_13165"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXX02160.1"
                     /translation="MSVLKTSAPAKETVTAEMAHWAAALRYEDISEDAVYQAKRFLLD
                     SIGCALGGYQQHDVKIALEVLDEVAGHGPATVIGTGRRIDPVSAALANALMIRCMDYN
                     DIYWKQDPSHPSDILPAAIACCERAGSDGKELILGLVLGHEFEMRLCEAAFPGIRERG
                     WHHATLTAFVSPIVAGRALHLSWEQIQHAIGISASRHCALGAVTAGKLTMMKNTVDPM
                     ATQSGVFAALLAEKGYTGPEHVIDGKEGLTHVFKPEWKLDVLTEGLGESWRITQCGMK
                     AFPTEALTHTPISAVLDLVKSNDLHPDQVEKIQIRSLARAADILSDPSKYDPHTKETA
                     DHSLPYVIAAALVERQVTPVQLTMEKILDPKIREQLTKVEVVADPEIEKVFPALQRVI
                     VNITTTDGRCFTKQLDYPKGDPRNPLTDQEIEEKFAALADRVLSKAAQERVKDAVWRL
                     EGVRSVSELMALMKADVREASSATRKTRPGTKALKHSKPVVA"
     gene            complement(1779..2339)
                     /locus_tag="VEJ00_13170"
     CDS             complement(1779..2339)
                     /locus_tag="VEJ00_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HXX02161.1"
                     /translation="MRETIKRLWPEIDWIQDLQLREQVTETWMRALARSPLKAEDLNR
                     IPFTLLVPNCPITFLEHKRCVVHIARESARAMQEFMGRALPVDMDTVIAGAILADVGK
                     LLEYELGPDGQSRQSERGEALRHPFTGVALALECGVPDAVCHIIAAHAAEGDLVKRTT
                     EAFIVHHADFMAFLPFKNPKNIKVKP"
     gene            complement(2453..>2959)
                     /locus_tag="VEJ00_13175"
     CDS             complement(2453..>2959)
                     /locus_tag="VEJ00_13175"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HXX02162.1"
                     /translation="TPLKRVSPICTFVGAFPGAMPGVLGWTAARGRLEWGTLVLFAIL
                     FVWQFPHFFSIAWLYREDYAQGGVRMLPVVEQDGRSTARRILAYSVALIPISLVPSFM
                     GMAGWVYFAGATVLGCAMLYFGIRLAFLNLAPASAPSKVRARHVLQATVIYLPLLFAL
                     MMANAVRS"
BASE COUNT          567 a          928 c          877 g          587 t
ORIGIN      
        1 ggcggcgaag gcttcgatcg accggtattt cttcagattg gcttcctcgc gattctggat
       61 gtcgtggtcc agcgcgaaaa ccagctgctt cgggtcgtag attttcttcg cgccgattgc
      121 cttgaacttg ctcatcaccg ccgacgagtt gtcgtgcgtc atcacgtgaa acggccggac
      181 ggacaggaag tctccggcac gcagcggccg tttcggtccg tcggccatgt gggcttgcgc
      241 gatcttctcg ataattgtct ggggcatggt ggactccagt ctcccgcgct atgcgaccac
      301 aggcttactg tgcttcaacg cctttgtgcc tggcctcgtc ttccttgttg cggatgatgc
      361 ctcgcgaacg tcggccttca tcagtgccat caattcgcta accgacctca ctccttcaag
      421 cctccaaacg gcgtctttca cgcgttcctg agccgctttc gacaacaccc gatcggccag
      481 cgccgcgaat ttctcctcga tctcctgatc ggtcagcgga ttgcgcgggt cgcccttggg
      541 atagtccaat tgcttggtga agcagcgtcc atccgttgtc gtgatattca cgatcacccg
      601 ctgcagggcg gggaagacct tttcgatttc cggatccgca accacctcaa ctttcgtgag
      661 ctgctcgcga atcttcgggt cgagaatttt ttccatggtg agctggaccg gcgtgacctg
      721 ccgctcgacc agtgccgcgg cgatcacata gggcagcgag tgatcggcag tttcctttgt
      781 gtgcggatcg tatttgctcg gatcggaaag aatgtcggcc gcccgcgcca gcgagcgaat
      841 ctgaatcttc tccacctggt cgggatgcag atcgttgctc ttgaccagat cgagcaccgc
      901 ggaaatcggc gtgtgcgtca gggcctcggt gggaaacgcc ttcatgccgc attgcgtgat
      961 gcgccaggac tcgcccaaac cctcggtaag cacatccagc ttccattccg gcttgaagac
     1021 gtgcgtgagc ccctccttgc cgtcaattac atgctccgga ccggtatagc ccttttccgc
     1081 caacagcgcc gcgaagacgc cgctctgggt cgccatgggg tcgaccgtgt tcttcatcat
     1141 ggtgagcttg ccggccgtga ccgccccgag tgcgcagtgc cggctggcgg aaatgccgat
     1201 ggcatgctga atctgctccc agctcagatg cagcgcgcgc cccgccacaa tgggcgacac
     1261 gaaagcagtc agggttgcgt gatgccagcc gcgctcacga attcccggaa acgccgcttc
     1321 gcacaatcgc atctcgaact catgaccgag aactagcccg aggatcaatt ccttgccgtc
     1381 gctccccgcg cgctcgcagc atgcgattgc cgccgggaga atgtcagaag gatgcgaagg
     1441 atcctgcttc cagtaaatgt cgttgtagtc catgcagcgg atcatgagcg cgtttgccag
     1501 cgccgccgag acggggtcga tccgtcggcc ggtgccgatc accgtggctg gtccgtggcc
     1561 ggcgacctcg tccagaacct ccagagcgat cttcacgtca tgctgctggt agccacccag
     1621 tgcgcagccg atcgagtcga gcagaaaccg cttggcctga tagaccgcat cttcggagat
     1681 gtcttcgtac cgcagagcgg cggcccagtg cgccatctcc gctgtcaccg tttctttggc
     1741 cggagcactc gttttcagca cagacacggt tgcctcctct agggcttcac cttgatgttc
     1801 ttcgggttct tgaatggcag aaacgccatg aaatcagcgt gatgcacgat gaaggcttca
     1861 gtagtccttt tgaccaggtc gccttccgcg gcgtgcgccg cgatgatgtg gcagaccgcg
     1921 tccggcacgc cgcactcgag cgccagcgcg acgcccgtga acggatgtcg cagcgcttcg
     1981 ccgcgctcgc tctgccggct ctggccgtcc ggcccaagtt cgtactcgag aagcttgccc
     2041 acgtcggcca gaatcgcgcc agcaatgacc gtatccatgt ccacgggcaa cgcccgcccc
     2101 atgaattcct gcatggcgcg ggcactctcc cgcgcgatgt ggacgacgca gcgtttgtgc
     2161 tccaggaagg tgatggggca attcgggacg agcagggtga agggaatgcg gttcaagtct
     2221 tcggccttca gcgggctgcg cgcgagcgcc cgcatccagg tctcggtaac ttgttcacgc
     2281 agttggagat cctgaatcca gtcgatctcc ggccagagcc gcttgatagt ctcccgcata
     2341 cctatccctg agcgcagaca tttcaggcta gcttcggagg gagtcagaaa cgacgattag
     2401 gatcacactg gcctgtgatt tcaacacgtc agaggtaggt tgatggggca gctcaggaac
     2461 gcactgcgtt ggccatcatc aaggcaaaga gaagaggcag atagatgacc gtagcctgca
     2521 ggacgtgacg agcccgcact ttcgacgggg cgctggccgg cgccaagttg agaaaagcca
     2581 aacggatccc gaagtacagc attgcgcaac ccaataccgt ggccccggca aagtagaccc
     2641 agcccgccat gcccatgaag ctgggcacaa ggctgatggg aattagcgcc accgagtaag
     2701 ccaggatgcg gcgagctgtg gagcgaccgt cctgctcgac caccggcagc atccgcacgc
     2761 cgccctgggc gtaatcctcg cgatagagcc acgcaatcga gaagaagtgt gggaactgcc
     2821 acacgaacag gatggcgaac aggaccagcg taccccactc cagccgcccg cgagcggcgg
     2881 tccagcccaa aacgcccggc atggcgccgg gaaacgctcc gacgaaggtg caaataggag
     2941 aaacccgctt cagcggagt
//
LOCUS       DASPUL010000565         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_249263, whole genome shotgun sequence.
ACCESSION   DASPUL010000565 DASPUL010000000
VERSION     DASPUL010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249263"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VEJ00_13180"
     CDS             <1..244
                     /locus_tag="VEJ00_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02163.1"
                     /translation="QRVRSLTEEFHLVLHTSPSTLHPSKNLGYWKTIDDDYHWHIEIL
                     PVLGSKARSYTFKEVYYSPVSSETAVKQLREAKIDG"
     gene            complement(299..2143)
                     /locus_tag="VEJ00_13185"
     CDS             complement(299..2143)
                     /locus_tag="VEJ00_13185"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXX02164.1"
                     /translation="MLLAWYWIPLATLAILALYQQLRLYHIRRDNKKREELFQIVTEN
                     AADMIALVDVKGRRLYNSPAYKRILGYSAAELGETSAFEQIHPEDRFQVLEAAREARE
                     SGVGRRLEYRIRHKDGSWRVLESVASAVRNEKGEVAKLVIVNRDITERKRAEEKLEHN
                     LFHDPLTGLPNRRLFLDRLRNAFLQTKRDSERQYALLLANVDHFKVFNERLGTAAGDQ
                     VLQEIARRVGTCLRQGDTVFRGGNDIAEPVLSRLGSDEFTILLDAVSDPSDAMRVATR
                     VQSAVAEPLLVEGQEVRISFSVGIALSAPTHEQPENMLKDADTAMRRAKALGGSRCEV
                     FDEAMHSRAMGRMRLEADLRTAIAQRQFRIHYQPVLELATQRVVSLEALLRWEHPTQG
                     LISPHRFLDAAEDTGLLVSIGHWLVMQACRQLSEWEITNSAGSPVSLTLNVSARQFAD
                     ARLVNDIQDALQETGVNPSRLQLEMTESVAAADPKLTVTVLAHLKHMGIGVILDDFGV
                     GSASLRGLWQFPVDALKIDRSLVREMLSDRAAASIVEMILTLGHKMGLRVIAEGIETV
                     RQAERLRELGCEYGQGYYFSQPLESKAALQFLRRQVVPARATGVGRSS"
     gene            2361..2723
                     /locus_tag="VEJ00_13190"
     CDS             2361..2723
                     /locus_tag="VEJ00_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02165.1"
                     /translation="MTVRRLKTYTGAQGYVYQYYFVGKRPSLPGDPETPATEYVFDVT
                     SDRKLTYAVSVFLPEGPLAAWSAAHGRPLSEAEQYAAVKLRLFRAFDELEDVKARGRR
                     LSIDAALLEEMLSGLGVD"
BASE COUNT          536 a          859 c          841 g          539 t
ORIGIN      
        1 gcagcgcgtg cgttcgctca ccgaggagtt ccatctcgtg ctgcacacct cgcccagcac
       61 gctgcatccg tccaagaatc tcggttactg gaaaaccatc gatgacgact accactggca
      121 catcgagatt ctcccggtgc taggctccaa ggcgcggtcg tacacgttca aagaggtcta
      181 ctattcgccg gtgagctcgg agactgcggt caagcagctg cgagaggcaa agatcgatgg
      241 ctaagacgat gctaggaatt tggtaatccg gagtagcccg ttcggagtat taccaggttt
      301 agctgctccg gccaactccg gtggccctcg ccggaaccac ctggcggcgc aagaattgca
      361 aagctgcttt ggactccagg ggctgcgaaa agtagtaacc ttgcccatac tcgcatccca
      421 gttcccgcaa acgctcggcc tggcgcaccg tttcgatgcc ctcggcgatt acgcgcaagc
      481 ccatcttgtg cccgagggtg aggatcattt cgacgatgga ggcggccgcg cgatcactca
      541 acatttcgcg cacgagcgag cggtcgatct tgagtgcatc gaccggaaac tgccacaatc
      601 cgcgcaagga tgccgagccc actccgaaat cgtcaaggat cacgccgatc cccatgtgct
      661 tgaggtgagc cagcacggtc accgtcagct tgggatcggc ggcggcgacg ctctcggtca
      721 tctccagttg cagccgcgat gggttgacgc cggtttcctg cagggcatcc tgaatgtcgt
      781 tgaccaggcg ggcgtcggcg aactgccgcg ccgagacgtt gagcgtcagg cttaccggcg
      841 accccgcgga gttggtgatc tcccattcgc ttagttggcg gcaggcctgc atcaccagcc
      901 agtgcccgat cgagaccagc aggcccgtgt cctcagcggc gtcgaggaag cggtgcggtg
      961 aaatcagccc ctgcgtggga tgttcccagc gcaacagcgc ctccagactg acgacgcgct
     1021 gcgtagcgag ctcaagcaca ggctgatagt gaatccggaa ctgccgctgc gcgatcgccg
     1081 tgcgcaggtc ggcctccagg cgcatgcggc ccatggcgcg gctatgcatg gcttcatcga
     1141 agacctcgca gcgcgatccg cccagcgcct tggcgcggcg catcgccgtg tcggcgtcct
     1201 tcagcatgtt ttccggctgc tcgtgggtgg gcgcgctcag ggcaatcccc acgctgaagg
     1261 agattcgaac ttcctgtcct tcgacaagca acggctcggc cacggcagac tgaaccctgg
     1321 tcgctacccg catggcatcg ctggggtcgg agaccgcgtc cagcaggatg gtgaattcgt
     1381 cgctgcccag ccgcgacagc acgggttcag caatgtcatt tccaccgcgg aataccgtat
     1441 cgccctgtcg cagacaggtg cccacgcgac gagcaatctc ctgcagcacc tggtcgcccg
     1501 ccgcggtgcc cagacgctcg ttgaaaacct tgaagtgatc gacgttcgcc agcagcaggg
     1561 catactggcg ttcggagtcg cgttttgtct ggagaaacgc gttccgcaac cggtcgagaa
     1621 acaggcgccg gttgggcagt ccggtcagcg gatcgtgaaa cagattatgt tcgagtttct
     1681 cctccgcccg cttgcgctcg gtgatgtcgc ggttcacgat gaccagcttg gcgacctcgc
     1741 ccttttcgtt gcggacagcg ctggccaccg actccagcac tcgccagctg ccgtccttgt
     1801 ggcgaatgcg atactccagc cgcctgccca cgccgctctc gcgcgcctcc cgcgccgcct
     1861 ccagaacctg gaagcggtcc tcgggatgaa tctgctcgaa ggcagacgtc tcgcccagtt
     1921 ccgccgcgga atagcccagg attcgcttgt aggccgggct gttgtagagc cggcggccct
     1981 tcacatcaac cagagcgatc atgtcggccg cattctcggt gacgatctga aacagctcct
     2041 ctcgcttctt attgtcgcga cggatgtggt agagccgcag ctgctggtac agcgcgagaa
     2101 tagccagcgt cgcgagggga atccaatacc aggccaaaag cataaggttc ttctgaggag
     2161 aactatcggc aaaatggagg gggagattta gctcggggca ccagatggca aaggattgct
     2221 gatggctgat tttcgattgt tgaatgaaac cctctcgcgt cggctggatc gttcgagatt
     2281 ttcattcaac aatcaacaat tccaggtacc gtctccggga gtgtaagaca aagccctcgc
     2341 gggcgttcga taatgtccta atgaccgtcc gccgcttaaa gacctacacc ggcgcccagg
     2401 gatatgtgta tcagtactac ttcgtgggga aacgtccctc gcttcccggc gaccccgaga
     2461 ctccggcgac cgagtacgtc ttcgatgtga cttccgaccg caagctgact tacgccgtca
     2521 gcgtgtttct tcccgaaggg ccgttggccg catggagcgc cgcacacggc cgtccgctca
     2581 gcgaagccga gcaatacgct gccgtgaaac tgcggttgtt tcgtgctttc gatgaactgg
     2641 aagacgtaaa ggcccgcgga cgccgcctga gcatcgacgc cgcgctgttg gaggaaatgt
     2701 tgtctggcct gggcgtggac taggacaggc cgccgaaaag ctgcggtaat gtctaagttg
     2761 aaggcctagg aggtg
//
LOCUS       DASPUL010000566         3896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1752331, whole genome shotgun sequence.
ACCESSION   DASPUL010000566 DASPUL010000000
VERSION     DASPUL010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1752331"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="VEJ00_13195"
     CDS             complement(<1..1350)
                     /locus_tag="VEJ00_13195"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXX02166.1"
                     /translation="MRSHSALARLGLGFPGWSSPGIAAALISTLLVCALGSGIAWGQA
                     TTSVRGTVTDPDGNAVAGAKVVIANAESKTERTATTGDQGEYQFLLIPPGTYTLGVTA
                     PGFRGYEQKGLTLLVNTPATANVQLKVGAAAELVTVTSEIPAIDLVDASLGNSFDERQ
                     VRQIPLEGRNVPDLLSLQAGVAYTGNRIGDKDQDTRNGAVNGARSDQSNITLDGVDVN
                     DQSNGYAFTSVLPVTQDSVQEFRVTTTNYGADQGQGSGAQVALVTKSGTNALHGALYE
                     YLRNTITSANDYLVKQSELNIGLPNKPLQLNRNIFGASVGGPIQKDRLFFFANYEGTR
                     EREQQRAERVIPTPSMCQGNLQYLYLTDPTNPNSSVATMTLTPTNLQQLDPKGFGIDM
                     PAMLDPVNHTGYLDTTFCSGRTPTNDPAAGDGLNYAGYTFRAPTSLDNDVFIARVDYR
                     "
     gene            complement(1479..1919)
                     /locus_tag="VEJ00_13200"
     CDS             complement(1479..1919)
                     /locus_tag="VEJ00_13200"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXX02167.1"
                     /translation="MSSPSNHGRENSELRELLHLTIPADPEAVASASDAVAGTLEQLA
                     VPEQKRLEIMLAVQEALTNAVAHGCDNDSSKNVACRLECDDEGRILIIVSDPGPGFDP
                     DSVAAPTHGERLHADHGRGVYLIRQLMDEVRFARSGSEIRMWKY"
     gene            complement(1966..2304)
                     /locus_tag="VEJ00_13205"
     CDS             complement(1966..2304)
                     /locus_tag="VEJ00_13205"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXX02168.1"
                     /translation="MDINVRRRSQVEVIHLRGQLRMGPAVDSLRQTIDEAFSSGDTRI
                     VIDLAEVPMIDSSGIGLLVKYLASAKQNGGSIKLVQPCKFVVQTLRLVGVLKLFEVHD
                     GEEAAVNSFS"
     gene            2512..>3896
                     /locus_tag="VEJ00_13210"
     CDS             2512..>3896
                     /locus_tag="VEJ00_13210"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXX02169.1"
                     /translation="MPATSTSQRPARVALVCSDDSVLPMVEESLAPFFHITYLKAPRE
                     VVLLHQETPLDAVVLDIDPVEPDAADSSDVLRDLRTMDRDLVLYAITRVRTRQHRLRI
                     HAAGADLVLAAPVDCQELKVKLEQSLEERQREIEQRNLEEQARSRSSFCDLVGASEAM
                     RNIYSAIGRVAESSTTVCIRGESGTGKELVARAIVATSPRRDRPFISLNCAALPETLI
                     ETELFGHEKGAYTDAHEARCGHFEAAQGGTIFLDEISSLGLGLQSKLLRVLEEHTVQR
                     LGARKATKVDFRLLTASNDDLEELVRAGRFREDLYYRINVVPIFMPPLRDREGDIALL
                     AEHFMRMYCAADGRPLKNLEPEVVEVLEEYAWPGNVRELENLMRRLVLMSEGSLIRVK
                     HLPQHILHQASARQEALLIPEEGIDFDQELENTEVAYLNAALKRTQGNKAAAARLLHV
                     NGQRMKYLCRK"
BASE COUNT          752 a         1130 c         1218 g          796 t
ORIGIN      
        1 ccggtagtcc acgcgcgcga tgaagacgtc attatcgagg ctggtgggcg cacggaaggt
       61 gtagcccgcg tagttcaacc cgtcgcctgc tgcaggatcg ttggtcggcg ttcgcccgct
      121 gcaaaaggtg gtatcgagat agcccgtgtg gttgaccggg tccagcatcg cgggcatatc
      181 aatcccgaaa cccttggggt caagctgctg gaggttcgtg ggggtcagag tcatcgttgc
      241 cacggagctg ttcggatttg tcgggtcggt gagatagaga tactgcagat tgccctggca
      301 catggacggc gtaggaatga cacgttccgc gcgctgttgc tcgcgctcgc gcgtcccctc
      361 atagttggca aagaaaaaga gacggtcttt ctggatcggc ccgccgaccg atgcaccaaa
      421 gatgtttcga ttgagctgca agggtttgtt ggggaggccg atattcagtt ccgactgctt
      481 caccaggtaa tcgttggcgc tggtgatcgt gttgcggaga tattcgtaga gcgcgccgtg
      541 caacgcgttg gtgccgcttt tcgtaaccag tgccacttgc gccccggagc cttgcccctg
      601 gtccgcgccg tagtttgtgg ttgtgacgcg gaattcctgc actgagtctt gcgtgacggg
      661 cagcaccgac gtgaaggcat atccgttgga ctgatcgttg acgtcgacgc cgtcgagcgt
      721 gatgttgctc tgatcgctgc gcgcgccatt gaccgcgccg ttgcgcgtgt cctggtcctt
      781 gtcgccgatg cggttcccgg tataggccac gccggcctgc aagctcagca ggtcgggaac
      841 gttgcgccct tcgagcggga tctgccggac ctggcgctcg tcaaaggaat tgccgaggga
      901 agcgtccacc aggtcgatgg ccggtatctc ggaggtgacc gtcaccagct cggccgccgc
      961 gccgacctta agctgaacgt tggccgtggc cggggtattg accagcagcg tcagaccttt
     1021 ttgctcatag ccgcgaaatc cgggcgcggt cacgccgagg gtgtaggttc cgggaggaat
     1081 cagcaggaac tggtattctc cctgatctcc ggtcgtggcg gtgcgttcgg tcttcgattc
     1141 ggcattggcg atcaccacct tcgcgccggc gacggcattc ccgtcgggat cagtaacggt
     1201 gccccggacc gaagtcgtgg cctgtcccca ggcgatgccg ctgcccagcg cgcataccag
     1261 cagagtcgag atcaaagcgg ctgcgatgcc cggcgatgac caaccaggaa accccaagcc
     1321 gagacgggcc aaggcgctgt ggctgcgcat atcccctccc cgaaagcaac gatttttgaa
     1381 acccgagacc ttactaaaat tagggaaggt tgtacacctc gagattgggg ttgtcaagcg
     1441 aggaatcgac gcggggaatc cggccggcag ggcgcacgtc agtacttcca catcctgatt
     1501 tcgcttccgg agcgggcgaa tcgcacctcg tccatgagtt ggcggatcag gtacacgccc
     1561 cggccatgat cggcatgcag tcgctcgccg tgcgttggag cggcgacgga gtcgggatca
     1621 aaacctggtc ccgggtcgct cacgatgatg aggatacggc cctcatcgtc gcactcgagg
     1681 cgacaggcga cgttcttcga cgagtcgtta tcgcagccgt gcgcgacggc attcgttaac
     1741 gcctcctgta ctgccagcat gatctccaga cgcttttgct cgggaaccgc aagctgctcc
     1801 agggtacctg ccacagcgtc gctggccgag gcaacggcct ccggatcagc cgggatggtg
     1861 agatggagca actcacgcaa ctcactgttc tcgcggccat gattcgacgg agacgacatg
     1921 cgggttcact tgattctggc gcccggcacc tgacggcgcg gaagttcagg agaaggaatt
     1981 caccgcggct tcctcgccgt cgtggacctc aaacagtttg agcacgccca ccaggcgcaa
     2041 ggtctgcacc acaaatttgc agggttgcac cagcttgatg cttcctccgt tctgcttggc
     2101 ggaggcgagg tatttcacca gcaggccgat tccactggaa tcgatcatag gcacttcggc
     2161 gaggtcgatc acgatccgcg tgtctccact gctgaacgcc tcgtcaatcg tctggcgcag
     2221 gctgtccacg gcaggaccca tgcgcagttg gccgcgaagg tggatgactt ctacctgcga
     2281 gcgccttcgt acgttaatgt ccacgtactt ctcctcgatg atcttgattt cacaggttct
     2341 gcaacggggg tttagagttt gtccggcgga cgaaagcaca aattacggga tcacggcaag
     2401 ttttgcaagg ggagtattcc ttcccggagt caaaaaactc tcattcccgg gtgaaatttt
     2461 tactataatg cgcaggcttg ttggttaccc ctgcggagga agcctcatcc tatgccggcg
     2521 acctcgacat cccaacgtcc ggcgcgtgtc gcgctggtat gcagcgatga ctccgtgctg
     2581 cccatggtcg aggagtcgct ggctcccttc ttccacatca cgtacctgaa ggctccccgc
     2641 gaagttgttc tcttgcatca ggaaacaccg ctggatgcgg ttgtcctcga cattgatccg
     2701 gttgagccgg acgcagccga cagctctgac gtgttgcggg atctccgcac catggatcgc
     2761 gatctggtgc tctacgctat tacccgcgtc cggacccgcc agcatcggct tcggattcac
     2821 gccgcgggcg ctgatctggt gctggctgcg ccggttgatt gccaggagct caaggtcaag
     2881 ctggagcaat ctctggaaga gcggcagcgg gaaattgagc aacgcaacct ggaagagcag
     2941 gcacgcagcc ggtcatcgtt ctgcgacctg gtaggcgcca gcgaagccat gcgcaacatc
     3001 tacagcgcca ttggccgggt ggcggaaagt tccacgacgg tgtgcataag aggtgaaagc
     3061 gggaccggga aagaactggt agcgcgcgcc attgtggcca ccagcccccg tcgcgaccgg
     3121 ccattcatca gcctcaactg tgcggcgctt ccggagacct tgatcgagac cgaactgttc
     3181 ggccacgaaa aaggcgctta taccgatgcg catgaagccc gttgcgggca ctttgaagcg
     3241 gcacagggtg gaaccatctt tctcgatgaa atctcatctc tgggcctggg actgcagagc
     3301 aagctgttgc gagttttgga ggagcacacc gttcagcgtt tgggagcgag gaaggcgacg
     3361 aaggtcgatt tccgcctgct gactgccagc aacgacgatt tggaggaatt ggttcgagct
     3421 ggccgcttcc gcgaagatct ttactaccgc attaacgttg tccctatttt catgcccccg
     3481 ctgcgcgacc gcgaaggcga catcgctctg ctggcggaac acttcatgcg catgtactgc
     3541 gcggccgacg gacgtccgct caagaatctc gaaccggaag tggtcgaggt actggaggaa
     3601 tatgcctggc cggggaacgt gcgcgagtta gagaacctta tgaggaggct ggtgctcatg
     3661 tcggagggaa gcctgatccg cgtcaagcac ttgccccagc acatcctgca tcaagcgtcc
     3721 gcccgccagg aagccctgct aattccggaa gaagggattg attttgatca ggagctggag
     3781 aacacggaag tagcctatct gaacgcggcg ctcaagcgga ctcagggtaa taaggccgcg
     3841 gccgcccggc tgctgcatgt gaatgggcag cgcatgaagt atctgtgccg caagca
//
LOCUS       DASPUL010000567         5583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_249684, whole genome shotgun sequence.
ACCESSION   DASPUL010000567 DASPUL010000000
VERSION     DASPUL010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249684"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..453)
                     /locus_tag="VEJ00_13215"
     CDS             complement(37..453)
                     /locus_tag="VEJ00_13215"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXX02170.1"
                     /translation="MNTSAPTQATTLDADFLRTLEVERTQAIVARDMPAIERLHAPDY
                     ELISPPGRVMTRERYLSLIAAEPFYAKWEHGEMRVRFSEGMAAVRYRAKITFPSGRVV
                     DWWHTDIYELQAGSWKAVWSQATQLAPNSPPSTGAL"
     gene            complement(571..1224)
                     /gene="lepB"
                     /locus_tag="VEJ00_13220"
     CDS             complement(571..1224)
                     /gene="lepB"
                     /locus_tag="VEJ00_13220"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXX02171.1"
                     /translation="MKRALLKAWREYSGFAVFIVSMLIFRSALADWNVVPTGSMKPTI
                     LEGDRILVNKLAYDFRIPLTHISLYRFADPRRGDIVVFDSKAADTRLVKRVIGLPGDT
                     VEMRDNRLAINGIAARYSNIGHEADAIFAIESYLDISHKIELARAGASRFGTFGPVTV
                     PKDCYLVLGDNRDNSADSRFYGFIPRDEIVGSAKTVVLSLDYDDFYIPRAERFFHPL"
     gene            complement(1322..1696)
                     /locus_tag="VEJ00_13225"
     CDS             complement(1322..1696)
                     /locus_tag="VEJ00_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXX02172.1"
                     /translation="MFDHVKFGVSDFAASKAFFLRALEPLGVAVVSEGSPAYGVELSP
                     KGKASLCLYQTEEKPAHLHLAFTAENRRQVDAFYRAALQAGGRDNGAPGLRPNYHANY
                     YAAFVIGPDGHNIEVVCHEPEA"
     gene            complement(1757..2134)
                     /locus_tag="VEJ00_13230"
     CDS             complement(1757..2134)
                     /locus_tag="VEJ00_13230"
                     /inference="COORDINATES: protein motif:HMM:NF014687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HXX02173.1"
                     /translation="MSEPDPRIFFAAERTLLAWLRTGLTVIALGFVVARFGLFLRLLS
                     QAPQATPSHTQSGASAALGVLFVFVGAVAILVATVQHRRYLGTLSSADLPASYNRGFA
                     VALSTVVGVLGLLLAAYLIVSRP"
     gene            complement(2240..2716)
                     /locus_tag="VEJ00_13235"
     CDS             complement(2240..2716)
                     /locus_tag="VEJ00_13235"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HXX02174.1"
                     /translation="MLRLLMVALFVVPTLSVAATATITADVALQIINGCVAHAKGKGQ
                     SHAIAVYDAGGHPVALLRMDGNQPGVTEFAVQKAEAVASWHFSTAEMAASAKRTPGFA
                     SAPHVVTVAGGIPIYSSEDQYIGAVGVSGEAPEDDTACAEAGVKAAGLSISRKAKG"
     gene            complement(2853..3056)
                     /locus_tag="VEJ00_13240"
     CDS             complement(2853..3056)
                     /locus_tag="VEJ00_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02175.1"
                     /translation="MNVAQLFFGVVAGAVGLGYIVYGKRQAKLIPIVSGLLLCGYSYF
                     IESWVWLSVAGTVLLAAPFFFDF"
     gene            complement(3171..3542)
                     /locus_tag="VEJ00_13245"
     CDS             complement(3171..3542)
                     /locus_tag="VEJ00_13245"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXX02176.1"
                     /translation="MNEQDESQIRAALQASADAWNRGDLKGHLAIYDSSVTVMTKNGP
                     RPTIDAIEASFGQTYFVNGKPKQNLRMESVRVRALCEQSALVTGRFVLSGGTEPEQSG
                     WFSLVWVHTALGWRVVHDHTS"
     gene            complement(3778..4425)
                     /locus_tag="VEJ00_13250"
     CDS             complement(3778..4425)
                     /locus_tag="VEJ00_13250"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXX02177.1"
                     /translation="MRPILLDLDDTLVDDYGSTAVAFNAFVEAHRGLLYDRDVGVLLD
                     EWRTVFVKHWMRHERGEISFLDQRRDRVREFLSNEFTNAEADAALLPYLQAYEASWRL
                     LPGVEEFLERSADIPKVILTNGNREIQLRKVTVTGLWSHVLDVITPEDCGYWKPAHGM
                     FLAGAHRLGVAPGSCIMVGDDPIRDIAPARELGMACFEVERGRCRDALAGALSAA"
     gene            complement(4447..5037)
                     /locus_tag="VEJ00_13255"
     CDS             complement(4447..5037)
                     /locus_tag="VEJ00_13255"
                     /inference="COORDINATES: protein motif:HMM:NF022606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2938 domain-containing protein"
                     /protein_id="HXX02178.1"
                     /translation="MTVARDRVNTEATYFVHAVVVGLGATLFMDVWALFLTRAFSIPS
                     ANYCLVGRWLGHMPEGTFIHASISSAPQTRGECTIGWIAHYVIGAVYGLLLVAFVSGS
                     WLAQPTLLPALIFGIGSVLVPYLIMQPSFGLGIAASKAPQPMQARLRSLMAHAAFGVG
                     LYVCAVCVSHVLRVDAQALAQPDRSPAGFTRPLAAA"
     gene            complement(5093..5320)
                     /locus_tag="VEJ00_13260"
     CDS             complement(5093..5320)
                     /locus_tag="VEJ00_13260"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXX02179.1"
                     /translation="MTIEFFEESNAGTRTFPRSLVTRAGGFVFVSGQIARDQNGDVIS
                     GDIEAHTRQTMKNVARALAVAGEIEVIAYKP"
BASE COUNT         1210 a         1657 c         1688 g         1028 t
ORIGIN      
        1 cgcgcttcat ggctgcgcgg ctccgctagg ctagcctcac agcgccccgg tcgagggtgg
       61 ggagtttggc gccagctgtg ttgcctgaga ccagaccgcc ttccatgaac ccgcctgcag
      121 ttcatagata tccgtgtgcc accaatcaac gactctaccg gacggaaatg tgatctttgc
      181 ccgatacctg accgccgcca ttccctcgga gaagcgaact ctcatctcgc cgtgctccca
      241 cttggcatag aacggctcag ccgcaatgag cgacaagtag cgctcacggg tcatgacgcg
      301 ccccggcgga ctgataagct cgtagtcagg cgcatgaagc ctttctatcg ccggcatgtc
      361 gcgagcgacg atggcttggg tccgttccac ctccagcgtg cgaaggaaat cggcgtcaag
      421 cgtggtagct tgcgtcggag ccgatgtatt catggtgtag gcagagcccc agagtggaag
      481 tttgagaccc aaagaagcga gcgaaccgac ccgagcggac ggcgcgccag cgccgccggc
      541 gagcggtccg gttgagccgg aagtcagctt tcacaaggga tggaagaacc gctccgccct
      601 tggaatgtaa aagtcgtcgt agtcgagcga gagcacgact gtctttgcgc tccctacgat
      661 ctcgtcacga ggaatgaatc cgtagaatct ggagtcggcg ctgttgtccc gattgtcgcc
      721 cagcaccaga taacagtctt tcggaactgt aacaggaccg aacgtgccga atcggctcgc
      781 tccagcgcgc gccagttcga tcttgtgcga tatgtcgaga tacgactcga tcgcgaatat
      841 cgcatcggcc tcatgcccaa tattggaata gcgggccgcg atgccgttga tggccagacg
      901 gttgtctctc atctccacgg tatcgccggg caagccgatc acgcgcttta cgagtcgagt
      961 gtcggcggcc tttgaatcga agacgacgat gtcaccgcgc ctgggatccg caaacctgta
     1021 gagagagata tgggtaaggg gaatcctgaa gtcgtaggcg agcttgttca caagtatccg
     1081 gtcgccttcg aggattgtgg gcttcatgga acccgtggga accacgttcc agtcggccaa
     1141 ggcgctgcgg aagatcagca tcgagacgat gaagaccgca aaaccgctgt attcgcgcca
     1201 agccttcaga agagctctct tcatatccaa tctccggtcc tgtctcaatg aaagccaacg
     1261 tttgacatga ggggcggccg caggtgagtg ctcccgcttc cgggcgtctc tcgatggggg
     1321 gctaggcctc gggttcgtgg caaaccactt cgatgttgtg cccgtccgga ccaatgacga
     1381 aagctgcata gtagttcgcg tggtagtttg ggcgcaggcc aggcgcacca ttgtctctcc
     1441 caccggcctg cagagcagcg cgatagaaag cgtcgacttg ccggcgattt tcggccgtga
     1501 acgccaagtg aagatgcgcc ggtttctcct cggtttggta caggcacaac gaagccttgc
     1561 ccttcgggct aagctcgaca ccgtacgcgg gcgacccctc tgagacaaca gctacgccga
     1621 gcggttcgag tgccctgagg aagaacgctt tgctcgcggc aaagtcgctg actccgaatt
     1681 ttacgtggtc aaacacgagc tcttttgaag acacccgccc tcacacgcgt ccggttgagc
     1741 gatgggttgg gcctcttcat gggcgcgaaa cgataagata agcggcgagg agcagcccca
     1801 gcacgccgac gacggtagac agtgcaactg cgaacccccg gttgtacgag gctggcaggt
     1861 cggctgacga aagcgtgcca aggtaacgcc ggtgctgcac cgtggctacc agaatagcga
     1921 ccgccccgac gaagacgaag agcaccccca gcgctgccga ggctccagat tgggtgtggc
     1981 taggcgtggc ttgaggcgcc tgcgacaaca agcgcaggaa cagtccaaag cgggcgacga
     2041 cgaaaccgag cgcgattacg gtgagaccgg tgcgcaacca tgcgagaagt gtgcgctctg
     2101 cggcaaagaa gattcgcggg tcgggctcgc tcattgcgct tggacagagg ccttacgaac
     2161 gaaactaagc ggcgccgagc ggactggcaa cgcccgccgg cgagcggacg ggttgagcga
     2221 ggggttgggc gacattgcct cagcctttgg ccttacgact gatcgaaagg ccagccgcct
     2281 ttacaccggc ctctgcacag gcggtgtcat cctccggcgc ctcgcctgaa acaccaacgg
     2341 cgccgatgta ctgatcttct gacgagtaga tgggaatgcc gcctgcgacc gtaacgacat
     2401 gcggcgcact ggcaaagcca ggcgtccgtt ttgccgatgc cgccatttct gccgttgaga
     2461 agtgccagct tgccactgcc tcggccttct gcaccgcgaa ctcagtcacg ccaggttggt
     2521 taccgtccat gcgcagaaga gcaacgggat gcccgccagc gtcatagact gcgatagcgt
     2581 ggctctgccc cttgcccttg gcgtgcgcca cgcagccgtt gatgatctgc agggcgacat
     2641 cggccgtgat ggtcgccgtg gcagcgacgc tcagagttgg tactacgaac agggcaacca
     2701 tcagtaggcg aagcatagtc tcctccatcg ctacgtcgca gaggtcgccc gacgcatcga
     2761 gctaaccggc cccgggccgg cggcgcgtaa gcaccgccag cgagcgatgc ggcggagcga
     2821 ctggttaggc ctcttcatag gagacatggg cgctagaagt cgaagaaaaa cggcgcggca
     2881 aggagcacag taccagcaac ggagagccag acccagcttt cgatgaaata ggaatagccg
     2941 caaagcagga gcccggagac aatcggtatc agcttggcct gacgcttgcc atagacgata
     3001 tacccgagcc ccactgcgcc cgcaacgaca ccgaagaaca attgggcgac gttcaccgga
     3061 ggatgcctaa atggaccaag ctgacccgcc tcgaggcgac ggcgcgcaag cgccgccagc
     3121 gagcgcttcg gtggagcgag tggtcaggcc tcattacgtg aggcacggct ttacgacgtg
     3181 tggtcatgga caacgcgcca gccaagtgcc gtgtgtaccc agacaagcga aaaccaacca
     3241 gattgttcag gttctgtgcc gcccgaaagg acgaagcggc ccgtcacgag ggcagattgc
     3301 tcgcaaagtg ccctgaccct tacgctctcc attcgtaggt tttgcttggg ctttccgttc
     3361 acgaaatatg tttgcccgaa agacgcctcg atggcatcaa tcgttggacg agggccgttc
     3421 ttggtcatga ccgtgacgga agagtcgtag atcgcgagat ggcctttcaa gtcaccgcga
     3481 ttccaggcgt cggccgaggc ttgcaaggcg gcgcgaattt gtgactcgtc ctgctcattc
     3541 atgatgcttg tcgggctttg gctgggcccg gcgcaggcac ttagcagcag gcaacaactg
     3601 gcgacgagtg atgcggcaag ggcgcgtccc gtcactgtgg cggtatccat tttcgaggcc
     3661 taacgaacca agctaacctg ccgtgagcgg acggggcgcg gtccggttgg ccgagtggtt
     3721 aggccgctca tcgctgctgc gcctccagac ggcgagcggg tccggtgaag cgagagatca
     3781 ggctgcactt agtgcccctg ccaaggcgtc ccgacaccga ccgcgttcca cctcgaagca
     3841 cgccatgccg agctcgcgtg cgggcgcaat gtctcggatc ggatcatcgc ctaccatgat
     3901 gcaactgccc ggagcaacgc cgagacggtg ggcgccggcc aagaacattc cgtgggctgg
     3961 tttccagtac ccacagtctt cgggtgtgat gacgtccagc acgtgcgacc acaggcccgt
     4021 gacagtaacc ttgcgaagtt gtatttctcg attcccatta gtcagaatga cctttggaat
     4081 gtcagcggag cgctcaagga actcttcgac ccctgggagc agtcgccatg aggcttcata
     4141 cgcttgcagg tacggcagaa gagctgcatc agcctctgcg tttgtgaatt cattgctgag
     4201 aaactcgcga acacggtcgc gccgctggtc taggaaggat atttcaccgc gctcgtggcg
     4261 catccagtgc ttgacgaata cggtacgcca ctcatcgagc aatacgccga catcccgatc
     4321 gtacagaaga ccgcgatgag cttcgacgaa tgcgttgaac gcgacagccg tggacccgta
     4381 gtcatcgact agcgtgtcat caaggtcgag taggatcgga cgcacagcac agcctaacgg
     4441 acaaagctaa gcggcggcaa gcggacgggt aaagcccgcc ggcgagcggt ccggttgagc
     4501 gagagcttgg gcatcaactc gcagcacgtg gctcacgcaa acagcacata catagagccc
     4561 aactccgaac gctgcatgcg ccatcaagct tcgcaaccta gcctgcatcg gctgcggcgc
     4621 tttcgacgcc gcaatgccga gcccgaacga aggctgcatg attaggtacg gcactagcac
     4681 gcttccgatg ccgaatatca gggctggcaa caaggttggc tgcgcaagcc agctacctga
     4741 gacaaaggcg acgagcagga gtccgtagac ggcgccaatg acgtagtggg cgatccagcc
     4801 tattgtgcac tctccacgcg tttgcggagc gctagagata ctcgcgtgta tgaacgtgcc
     4861 ctccggcata tggccaagcc accgcccgac cagacagtag ttggccgacg gaatgctgaa
     4921 tgcacgcgtc aagaacagcg cccaaacatc catgaacagg gtggcaccaa ggccaacgac
     4981 aaccgcatgt acaaagtaag tggcttcggt gttcacgcgg tctcgcgcaa cagtcattat
     5041 ctcaaggcgc atcgtggacg cggacacgat gttgctgaag atgagatcgg cctcatggct
     5101 tataggcaat cacttcaatc tccccggcta ccgcaagtgc gcgagcgaca tttttcatgg
     5161 tctggcgcgt gtgagcttca atatctcccg agatgacgtc accgttttgg tctctggcta
     5221 tttggccgga gacaaacacg aagccgccag cccttgtcac caatgagcgc ggaaatgtgc
     5281 gagtgcccgc attactctct tcaaagaact cgatcgtcat ttatgacacc tctcgcgaag
     5341 aaggaggcct gtggtgtgca tcggaacgtc gcctaacgtt tgaggctcag cggcggacgt
     5401 agtctggcac agcaggccgc caccgataac ataaagcagc tcaacgctta tccttcgcca
     5461 ccacgctcgg tgtcggggct tcattcacga tcagctgaaa gacgtccggc cgggcgtagt
     5521 ggcccacaac atcgaagtcg aatttgctgc gcccaatctc attgagatcc agatctgccg
     5581 tca
//
LOCUS       DASPUL010000568         4579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1203490, whole genome shotgun sequence.
ACCESSION   DASPUL010000568 DASPUL010000000
VERSION     DASPUL010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203490"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1901)
                     /locus_tag="VEJ00_13265"
     CDS             complement(213..1901)
                     /locus_tag="VEJ00_13265"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXX02180.1"
                     /translation="MNSAALNRKFPAKTVERIALTLLVGACLVLPLRAQQATHGPDPG
                     ETPSVQTSVNPNNKQVNQELLQELESMKKRIEQLEAELKQRDSSGQQPANNEALASAS
                     ALVSPGLRAVPAPALPAAAVQAQGSEAAAPAKAAPAEPFAFADWSWLNGTPRTKEAAF
                     DSKFFTPEVRADVAYHYDFNHPKDDTIGGSSEVFRSNEISLTHLGVGGDFHYDHVRAR
                     VFSQLGLYSTATPRNDASYSRGQWQLDNAYRYLNEAYGGYHFDALHGINVDAGIFLSY
                     VGLFSFYQFDNWAYQPSYVSSNTPWFFTGMRIQVFPTEKLKIEPWIINGWQSYARPNG
                     RLGFGGQILYRPTPWMSIIANNYGAGADAVGIPNRTRYHTDDSIEIKYYDHPERFLDR
                     MAFSLTGDMGCETGGGVNCTTNKKDAAGNIIAFKQSFLGYMLYNRWWFHKDRYGLTIG
                     GGQINNPGRYLVLLPPIDGATATTGTPYFTENPGDPFKAWDMSGTYDYMPSQYITFRW
                     EFNHRHANVPYWSGPGGVTPPGGNNGNPGAIIPGWSPDLRKTEDRATMAILVKF"
     gene            complement(2210..2467)
                     /locus_tag="VEJ00_13270"
     CDS             complement(2210..2467)
                     /locus_tag="VEJ00_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02181.1"
                     /translation="MWSIRNVSAEQLAKLFHHYRGALAHDLDCQGGEEAGSSWDRAPQ
                     HERKLMVAAARLALLELATAPAPAGSDRQYFAKPGEAEWGC"
     gene            complement(2740..3450)
                     /locus_tag="VEJ00_13275"
     CDS             complement(2740..3450)
                     /locus_tag="VEJ00_13275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXX02182.1"
                     /translation="MPDPPHILVVDDEPQITRVLRTSLSAQGYDIRVANTGEMALEIM
                     KDWSPTLIITDLSMPSVDGVELCRKVRSVSQVPILVLSVRDQERQKVEALDAGADDYI
                     TKPFGMNELLARVRANLRRVAAENEPAEAVTEIGDFRIDTSAHKVTVRGQEVRLTPKE
                     FDLLVYLARHPGKVINHRTLLGAIWGGQSTEQVEYLRVFVGQLRKKLESDAASPRYIV
                     TEPWVGYRFEPGEDGHQG"
     gene            complement(3450..>4579)
                     /locus_tag="VEJ00_13280"
     CDS             complement(3450..>4579)
                     /locus_tag="VEJ00_13280"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HXX02183.1"
                     /translation="KAMQKSPEQWLEEVAPSKSQGILKLFLGYAPGVGKTYSMLSEAI
                     RRASRGEDVVVGVVESHGRKATAELAAKVDRIPPRKLEYKGTIFEEMDVDAILARKPA
                     VVVVDELAHTNIEGSKHAKRYEDVMELLDAKIDVLSTMNVQHVESVGPTVQQITGVQV
                     RETVPDWVMQRVDEIVLADLTPQALQKRMQRGDIYPVDRAQRALAHFFRPGNLIALRE
                     LALRQVTGVVDRSLDAFLEKDGKQPAHTVRERIGVCVSSNPAAQYLIARGSRMAEAMG
                     GELYVFYVDVGRDTRPEDQKTLVENIRFAENLGAHVVRTSGRSIADCVAQLVRENHIT
                     QVIFGRSARTGWQRYLYLSAIQRFLRDSPSVDVHIVTQEAR"
BASE COUNT          921 a         1377 c         1314 g          967 t
ORIGIN      
        1 tacaccgtta ccagcgcgct gatgatcagc atgattagag attgtgtgtc cataaccctt
       61 cctcacttca cccgatcgca aaagtgcaca tacgcgatcg acacggcaaa gaacgcaatc
      121 gtaacgacgg tccacaacaa gtcctgcata cgtgtctctc ctccagttcg gcgaagtcag
      181 ggatcagaat cgctcccgac cgatccctgc tgttagaact tcaccagaat cgccatcgtc
      241 gcccggtctt cggtcttgcg caggtcgggg gaccatcccg gaatgattgc tcctggattt
      301 ccgttattcc ctcccggagg tgttactccg ccagggccgc tccagtacgg cacattggcg
      361 tgacgatggt tgaattccca gcggaacgtg atgtactggc tgggcatgta gtcgtaggtt
      421 ccggacatat cccatgcctt gaagggatcg cccgggttct ccgtaaagta cggcgtgccc
      481 gtcgtggcag tggcgccgtc aattgggggc aacagcacga gatagcggcc ggggttattg
      541 atctggccac cgccgatggt cagtccgtag cggtctttgt gaaaccacca gcggttataa
      601 agcatgtacc cgaggaagct ctgcttaaag gcaatgatgt tgccggcggc atccttctta
      661 ttggtggtgc agttcacccc gccgcccgtt tcgcagccca tgtccccggt caggctaaaa
      721 gccatcctgt ccagaaagcg ctcgggatga tcgtagtact tgatttcgat actatcgtcg
      781 gtgtgataac gagtccggtt gggaattcca accgcatcag caccggcccc ataattgttc
      841 gcgataatcg acatccacgg tgtcggacgg tagagaatct ggcctccaaa tcccaatcgt
      901 ccattgggcc gcgcgtagga ttgccaccca ttgataatcc agggctcgat tttcagcttc
      961 tccgtcggaa agacctggat gcgcataccg gtaaaaaacc acggcgtgtt ggacgagacg
     1021 taagacggct gataggccca gttatcgaac tgataaaagc tgaacagccc cacgtaggac
     1081 aagaaaatcc cggcatcgac gttgatgcca tgcagagcgt caaagtgata gccaccgtaa
     1141 gcctcgttca gatagcgata agcattatcc aattgccatt ggccacgcga gtagctggcg
     1201 tcgttgcgcg gtgtggcagt tgaatacaga cctaactggc tgaagacgcg cgcacgaaca
     1261 tggtcgtagt gaaagtctcc gccgacacct aagtgggtaa ggctgatttc attcgaacgg
     1321 aatacctcgc tcgaaccgcc aatagtgtcg tccttggggt ggttgaaatc gtagtggtaa
     1381 gcaacgtcag cgcgaacctc cggcgtgaag aacttggaat cgaaagccgc ttctttcgtg
     1441 cgcggggttc cgttcaacca gctccagtca gcaaaggcga aaggctcggc cggcgccgcc
     1501 tttgccggcg cggcagcctc ggacccctgc gcctgaaccg ctgctgccgg cagcgcaggc
     1561 gcaggcactg cccggaggcc gggtgaaacc agagccgagg cgctcgccag ggcctcgtta
     1621 ttagccggct gctgtccgga gctgtcacgc tgcttgagct ccgcttccag ctgctcgatg
     1681 cgcttcttca tggattccag ctcttgcaga agctcctggt tcacttgctt gttgttcggg
     1741 ttgactgatg tctggacgga gggcgtctcg cccgggtccg ggccgtgcgt tgcttgctgg
     1801 gcgcgaagcg gaagcacaag gcatgctccc acgaggagcg tcagcgcgat ccgctccact
     1861 gtctttgctg gaaacttccg gttcaaagcc gcggaattca taggtatgag ttctcctcgt
     1921 tgctgcgatg gtttgaactt cgctaaataa accgtcaggg cgggatgggg atagcgtctg
     1981 cgttccttta ccagggcgat caggacccgc agcaggaacg tcaccgcgca accacacaga
     2041 acgaccacag cgagtcgcga gatttctggc agacttgtca cggaaaggta ctagatggga
     2101 ttgaataacc taggccataa aaaggggctt aggaattcgt aaagagttcg taaaggaacc
     2161 agaccagtct cagtaccccg tgatcaggat aaggcccgcc acgcgtgtgt cagcagcccc
     2221 attccgcctc gccaggcttg gcgaaatact ggcgatcgct gccagccggc gccggagcgg
     2281 tcgccagttc cagcagcgcc agtcgcgctg ctgccaccat cagtttgcgc tcgtgctggg
     2341 gagcgcgatc ccacgaagag cctgcctcct cgccgccctg gcagtccaga tcatgcgcca
     2401 aagcaccccg atagtgatga aaaagctttg ccagttgttc cgccgagacg ttccggatcg
     2461 accacatatc ctcgatgcct ttctgcgttt cgagccctgc ccgattcgcc cttcctgctg
     2521 cactcgctac attggatatc ccgactcgta aggattgaat aaaaagttcg tagaatgttc
     2581 gtaatcctcc ggagctcgct tcgaagccga gtgatctccg ccgagcggtg tgttgagctt
     2641 gacaaagggg tgcctcgcgg gctggtcagt attcgccgat tgaggtcacg tggaacagcg
     2701 gcccttcagg gccgcgttaa gtgcgtaaat gactcgggct tagccctgat ggccgtcttc
     2761 ccccggctca aaccggtacc cgacccaggg ttcggtcacg atatacctcg gtgaagccgc
     2821 atccgactcg agctttttcc gcagctggcc gacgaacacg cgcagatact ccacctgttc
     2881 cgtgctctgc ccgccccaaa tggccccaag cagagtgcgg tggttgatca cttttccggg
     2941 atggcgtgcc aggtacacaa gcagatcgaa ctccttcggt gtgaggcgca cctcctgccc
     3001 gcgaactgtc accttgtgcg ccgaggtgtc gatacgaaaa tcgccgatct cagtcacagc
     3061 ctcggcaggc tcattttccg ccgccacgcg gcgaagattc gcccgcaccc gcgccagcag
     3121 ttcattcatg ccgaaaggct tggtgatgta gtcgtcggcg ccggcgtcca gtgcctcaac
     3181 tttctgcctt tcctggtcgc gaaccgagag aacgagaatg ggaacctgcg agacggaacg
     3241 aacctttctg caaagttcca caccgtcgac cgacggcatc gacaagtcgg tgatgatcag
     3301 cgtgggcgac cagtctttca tgatctccag agccatctct ccagtgttgg cgacccggat
     3361 gtcatagccc tgcgccgaga gggacgtgcg cagcacgcgt gtgatctgcg gttcgtcgtc
     3421 tactaccaga atgtgcggag gatctggcat caccgtgcct cctgagtcac gatatgcacg
     3481 tcgaccgacg gcgaatcgcg cagaaaccgc tggatggccg acaggtacag atatcgctgc
     3541 cagccggtgc gcgccgagcg tccgaagatc acctgcgtga tgtgattctc gcgcaccagc
     3601 tgcgccacgc agtcggcgat acttctcccc gacgtccgca ccacgtgcgc tcccagattc
     3661 tccgcgaaac ggatgttctc aaccagcgtc ttttgatctt cggggcgcgt gtcgcgtccc
     3721 acatccacat aaaacacgta tagttctccc cccatggcct cagccatgcg cgagccgcgc
     3781 gcgatcaggt attgcgccgc aggattcgag ctgacgcaaa ctccgatgcg ttcccgcacg
     3841 gtgtgcgccg gctgttttcc gtccttttcc agaaacgcgt cgagactgcg atcgaccacg
     3901 cctgtaacct ggcgcagagc cagttcgcgc agcgcgatca ggtttcctgg ccggaaaaaa
     3961 tgggccagcg cccgctgcgc ccgatccacg ggatagatgt cgccgcgctg catacgcttc
     4021 tgcagcgcct gtggcgtcag gtcggcaagg acaatctcat cgacgcgctg catgacccag
     4081 tcgggcacgg tctctcgaac ctggacgccg gtgatctgct gcaccgtcgg cccgacgctt
     4141 tccacatgct gaacattcat ggtggagagg acgtcgattt ttgcgtcaag gagctccatc
     4201 acatcctcgt aacgcttggc gtgcttgctg ccttcgatgt tggtgtgagc caattcgtcg
     4261 accacgacga ctgcgggctt gcgggccaag atggcgtcga cgtccatctc ttcaaagatg
     4321 gtgcctttgt attcgagttt acgaggggga atgcgatcga ctttggcggc aagttcggca
     4381 gtggctttgc ggccgtgact ttcgacgacg ccgacgacca catcttcacc gcggctggcg
     4441 cggcgaatgg cctcactcaa catcgagtag gtcttgccga ctccgggagc gtagccgagg
     4501 aaaagcttga ggatgccctg tgatttggag ggcgcgactt cctccaacca ttgttccggt
     4561 gacttctgca ttgcctttg
//
LOCUS       DASPUL010000569         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_249830, whole genome shotgun sequence.
ACCESSION   DASPUL010000569 DASPUL010000000
VERSION     DASPUL010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249830"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="VEJ00_13285"
     CDS             complement(<1..269)
                     /locus_tag="VEJ00_13285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HXX02184.1"
                     /translation="MPIWLFILASVIKSAVVLVVLLTAVAYTVWLERKVVGHMQNRWG
                     PTRVGPFGLLQPAADGLKFLFKEDLTPPHVYRPLYIAAPLIAVIFA"
     gene            complement(318..>2202)
                     /locus_tag="VEJ00_13290"
     CDS             complement(318..>2202)
                     /locus_tag="VEJ00_13290"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXX02185.1"
                     /translation="GEGMDVWALGVQNRGVSSIIAPNKEDHLECEECGMCIDICPVGA
                     LTSGAYRYKTRPWEMKHVGTVCTHCGDGCKTTLGVRRSDTGMEIVRGDNRDKSGANGD
                     FLCVKGRYAFDFASHEERLTQPLIRINGKLTPSSWEEAFELVGKKFAWVRDQDGGAAI
                     GVIGSTRTTNEEAYLLSKFARTVLKTNNIDHHRTADFPALAAALRGKPNATATMADVY
                     NAPAILLIGNDPTEQHPLLAWQMRNNVRLHRARLHVINSVPIKLRRQAANFMQIPAGT
                     EGKVASFLAGSDSSLDELTGPAASKDAWLALRDKLRAEQNLVIIFGAEIRGGDLASLV
                     AFGSSIPGAKFVCLADYSNSRGAADMGLYPDLLPGYHPTVGNSPFHQEWGEIPQDTGL
                     DLPGMVSAAKDGKLKALYVVGSNPVGRLNLDPFVFSKTFVVVQDMFLTETATLADVVL
                     PAANAYEKSGTVTNTCGDVQLAKKAGEVSGTKADFEMIVRIADAMDVDVHKLVPFGGG
                     THADMGQSRGAQSGEADRHAVWLEMQGLEPKLSPLDPMAILDEIQRLVPGYGVSRMNL
                     LAGNSEHTSLEKCGLGAAHKPEAIVPAHDGLFTSGTLGRYSRALKSVVESHEVKPAAV
                     AAD"
BASE COUNT          426 a          675 c          677 g          424 t
ORIGIN      
        1 gcgaagatca ccgcgatcag cggcgccgca atataaagag gccggtagac gtgcggcggt
       61 gtaaggtctt ccttgaacaa gaacttcaat ccatccgcag caggttgcag caggccgaac
      121 gggccgacac gcgtaggacc ccaacgattc tgcatgtggc cgacgacttt gcgttcaagc
      181 cataccgtgt aggcgacggc ggtgagcaat acaacgagca caacggccga cttgattacc
      241 gaagcgagga tgaagagcca gatcggcaac gcttacgccc tctccgtaac tcggactgcg
      301 atggtggaac tcgtgaatca gtctgctgcg acagcagcag gtttgacctc gtgactctcc
      361 acgaccgact tcaaggcgcg ggagtaacgt ccaagagtgc ccgaagtaaa caagccatcg
      421 tgtgccggaa cgatcgcctc cggcttatgc gccgccccga ggccgcactt ctcgagagaa
      481 gtgtgctcgc tgttcccggc cagcaggttc atgcgcgaga cgccatagcc cggaaccagc
      541 cgctgaatct cgtcgagaat tgccatggga tcgagcgggc tcagcttcgg ctccaggcct
      601 tgcatttcaa gccagaccgc atggcggtcg gcctcgccgg actgcgctcc gcgcgactgt
      661 cccatgtcag catgcgtgcc gccgccaaag ggcacgagct tgtgcacgtc aacgtccatg
      721 gcgtcggcaa tgcggacgat catttcaaaa tccgctttcg tgcccgatac ttcacccgcc
      781 ttcttcgcga gctgcacatc gccgcaggtg ttggtcacgg tgccggattt ttcataggca
      841 ttggctgcgg gcaacaccac gtctgccagg gtcgcggtct cggtcaggaa catgtcctgt
      901 accacgacga aggtcttcga gaaaacgaag ggatcgaggt tgaggcggcc gactggattg
      961 gacccgacga cataaagagc tttcaacttg ccgtccttgg cggcactcac catgccagga
     1021 agatcgagtc cggtgtcttg cggtatttcg ccccattcct gatggaacgg gctgttccct
     1081 acggttgggt gatagccggg cagcagatcg gggtagaggc ccatgtcggc tgcgccgcgc
     1141 gagttggaat agtcggcgag gcatacgaac ttcgcgccgg gaatgctgga gccgaaggcg
     1201 acgagactgg ccaggtcacc tccgcgaatc tctgccccga agatgatgac gagattctgc
     1261 tcggcgcgca gtttatcccg aagggcgagc caggcatcct tgctggccgc gggacccgtc
     1321 agctcgtcga gcgacgaatc gctgcccgcc agaaagctcg caaccttacc ctctgttccc
     1381 gccggaatct gcatgaaatt tgcagcctgg cggcgcaact tgatgggcac cgagttgatg
     1441 acatggagcc gggcgcggtg caggcgcacg ttgttccgca tctgccaggc cagaagcgga
     1501 tgctgctcgg tcgggtcgtt gccgatcagc aggatcgccg gggcattgta gacatcagcc
     1561 atcgttgcgg tggcgttcgg cttgccgcgc agagccgccg caagggcagg gaagtccgcc
     1621 gtccggtgat ggtcgatatt gttcgtcttc agcaccgtgc gcgcgaactt cgagagcagg
     1681 taggcttcct cgttcgtggt gcgggtcgag ccgatgactc cgatggctgc gccaccgtcc
     1741 tggtcacgaa cccaggcgaa ctttttgccg actaactcaa aggcctcttc ccaactggac
     1801 ggagtcagct tcccatttat gcgaatcaag ggttgcgtca ggcgctcttc gtggctggca
     1861 aagtcgaaag catagcgccc tttgacgcaa agaaaatctc cattggcgcc gcttttgtcg
     1921 cggttgtcgc cgcggacgat ctccatgccc gtgtcagacc ggcgcacgcc cagcgtggtc
     1981 ttgcagccgt cgccgcagtg cgtgcagacc gtgccgacat gcttcatctc ccaggggcgt
     2041 gtcttgtagc gataagcgcc cgaggtgagc gcgccgaccg ggcagatgtc gatgcacatg
     2101 ccgcactctt cgcactcgag atgatcttcc ttattcggcg cgatgatcga actgacgccg
     2161 cggttctgca cgcccagcgc ccagacatcc atgccctcgc cg
//
LOCUS       DASPUL010000570         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_658193, whole genome shotgun sequence.
ACCESSION   DASPUL010000570 DASPUL010000000
VERSION     DASPUL010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658193"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VEJ00_13295"
     CDS             complement(<1..250)
                     /locus_tag="VEJ00_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02186.1"
                     /translation="MRTPQIILVAGLMVLFVLLVTPQRHPYAYNAAHEVTIAGVVEDV
                     RDFYCPVNGAVGAHLIIATDTGKVQVHVAPVQFLDSQQR"
     gene            482..964
                     /locus_tag="VEJ00_13300"
     CDS             482..964
                     /locus_tag="VEJ00_13300"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HXX02187.1"
                     /translation="MPLTKLREFLDSHDVKYLVISHSVAYTAQGIAALVHLSGKRLAK
                     TVIVKIDGALAMAVVPASLHVDLDLLRVASDAHMVELATEREFKDAFPDCETGAMPPF
                     GNLYNMPVYADSSLAENEEITFNAGTHRELMRMAWADMVRLVNPRIVNLTYRPAIVAA
                     "
     gene            complement(1200..2021)
                     /locus_tag="VEJ00_13305"
     CDS             complement(1200..2021)
                     /locus_tag="VEJ00_13305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXX02188.1"
                     /translation="MSDDSNRELLHHYTDADEAARLRTGWFQLEHARTQELILRHLPN
                     APAKIIDAGGGAGAYACWLAARGYEVHLIDPVPKHVEQARAASARQPQYPLASAEVGD
                     ARHLSHTDSSADAVLLLGPLYHLVEREDRLACLREAHRVLRTGGLVWAAGISHFASLF
                     DSLSHGFFDDPAFAPILARDLEDGQHRNPTANPIYFTDAFFHRPGELSRELLAAKFKV
                     EEVSAIEGPGWLARDFDRLWADPAQRERLLAVVRKVEREPSVLGVSSHIMAIGRK"
     gene            complement(2135..>2930)
                     /locus_tag="VEJ00_13310"
     CDS             complement(2135..>2930)
                     /locus_tag="VEJ00_13310"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXX02189.1"
                     /translation="PGNHESPSVAVERALAMLEAVAQEPEGLSNAEISRKLQIPKSSA
                     SYILRTLVTRAYLNRDDGTGKYRVGLKILSLSRGALSGLDVREVALPIMRHLTERTGL
                     TCHLAILDGPEAVYIEKVEPQGFLRVDTWVGRRMRVHATSVGKALVAHIPQEQLEKIV
                     AESGMERRTPKTITTMPRLLKELEKVRAQGYAVDDEENNLGARCLGAPIFNQSGAIEA
                     SLGLSGTINQVNAHTMARIVEALKDAARHVSMQLGYRPPHRRAGGI"
BASE COUNT          528 a          859 c          964 g          579 t
ORIGIN      
        1 gccgctgctg actgtcgagg aactgaaccg gagccacgtg cacctgcact ttgccggtgt
       61 cggtggcgat gatcaagtgg gcgcccacgg cgccgttgac agggcaatag aaatcgcgaa
      121 catcttctac cactccggca atcgtcactt cgtgcgccgc gttataggcg taaggatgac
      181 gctgcggtgt aaccagcagt acgaacaaca ccatcagccc ggcaacaaga atgatctgtg
      241 gagtcctcat tgagatactc ctttccccct agcttgctcg gcagcttgat tctcggatgg
      301 tgcccgtgaa tctgccgtga cccggatcac gatggcgctg tggaaaaggg gtgcacttct
      361 cgctacggcc gtgatcgtgg tcaccgaaat cgcgcgggct gggaatagaa tggtggcggc
      421 aaagaaaagc cgcgcaagcg gacccctcaa cttcgtttcc ttccatcagc gaggaggtct
      481 tatgccgctg accaagcttc gtgaatttct ggactcccac gatgtgaagt acctggtgat
      541 ctcacactcg gtcgcgtaca cggcccaggg gattgccgcg ctggttcatc tttcgggcaa
      601 gagactggcg aaaacggtga tcgtaaaaat tgacggtgcg ctggctatgg cggtcgtgcc
      661 ggcgtcactg cacgtggacc tcgacctgct gcgggttgcg tccgacgcgc acatggtaga
      721 actggcaacc gagcgcgaat tcaaggatgc gttccctgac tgcgagaccg gagccatgcc
      781 gccgttcggc aacctctaca acatgccggt ctatgccgat tcgagcctcg ccgagaacga
      841 ggagattacg ttcaacgcgg gcacgcatcg cgaactgatg cggatggcat gggcggacat
      901 ggtgcggctg gtgaatccgc gcatcgtcaa tctgacgtat cggccggcga ttgtcgcagc
      961 gtaggctcgg acgctcggcg aggcagcaaa aggaagcagg gtatttctgt cggctgccaa
     1021 tcgacctcct ctcttcccaa cgtgtcatgc cgagcggagc tgcttttcaa gcggagcgag
     1081 ggatcttgcg cgcgccatgg cggcttcgct ccacgcgaga tccctcggcc cgctggtaaa
     1141 agcgtgggcc ttcgggatga cgcctcagat gcccgaaccg ggctcctgga gacctaacgc
     1201 tattttcttc cgatggccat gatgtgcgag ctcacgccga gcaccgaagg ctcgcgctcc
     1261 accttgcgca cgaccgcgag caggcgctcg cgctgcgccg ggtcggccca caagcggtcg
     1321 aagtcgcggg ccagccagcc gggaccttcg attgccgaca cttcttctac cttgaatttt
     1381 gccgcaagta actcgcgtga caactcgccg gggcggtgga agaacgcgtc ggtgaagtag
     1441 atgggattcg ccgtcgggtt gcggtgctgt ccgtcttcga gatcgcgggc gagaatcgga
     1501 gcgaaggcgg gatcgtcgaa gaacccgtgc gacaacgaat cgaatagcga cgcgaagtgg
     1561 ctgatgcccg cggcccagac aaggcctccg gttcggagaa ctcggtgagc ttcgcgcagg
     1621 caggccaggc gatcttctct ttcgaccaga tgatagagcg gaccgagcag caggacggcg
     1681 tcggcggaag agtccgtgtg ggaaagatgg cgggcgtcgc cgacttcggc ggaagccagc
     1741 ggatattggg gctggcgggc ggacgcagcc cttgcctgct caacatgctt gggcacggga
     1801 tcaatcaggt gtacttcgta tccccgggct gctagccagc aggcgtaggc gccggctcct
     1861 ccgcccgcgt cgatgatctt cgctggagca ttcggcaggt gacggagaat cagttcctgc
     1921 gtgcgcgcgt gctcgagctg gaaccagccg gtgcgaaggc gcgcagcttc gtcggcgtcg
     1981 gtgtagtgat ggaggagctc gcggtttgaa tcgtcgctca tggtgtcctt tgcggacttc
     2041 gcgaatcctt agcgctcttg gcggtgagca gctaccgcaa agttcgcgaa gaaatgccgc
     2101 ggaggacgca gaaacgtatc caaatcaggg tcgtctatat gccgccggct cgccggtgcg
     2161 gcgggcgata gccgagttgc atggaaacgt ggcgggctgc atccttcagc gcctcgacga
     2221 tgcgggccat ggtgtgggcg ttgacctggt tgatggtgcc gctgaggccg aggctggctt
     2281 cgatggctcc gctctggttg aagattggtg cgcccaggca gcgcgctccc aggttgttct
     2341 cctcgtcgtc gacggcatag ccctgcgcgc gcaccttttc gagttctttc agcaggcgcg
     2401 gcatggtggt gatggttttc ggggtgcgcc tctccatgcc ggattcagcg acgatcttct
     2461 ccagctgttc ctgcggaatg tgggctacca gcgccttacc cacgctggtg gcatgaacgc
     2521 gcatgcggcg gcccacccag gtgtcgacgc ggaggaatcc ctgcggctcg accttttcga
     2581 tatagaccgc ttcgggaccg tccagaattg cgaggtggca ggtaagaccg gtgcgttcgg
     2641 tcaggtggcg catgatgggg agtgcaactt cccgcacatc gaggccgctc agcgcgccgc
     2701 ggctcaggct gaggatcttc aggcctaccc ggtatttccc ggtaccgtca tcgcggttca
     2761 ggtaagccct ggtgaccaag gtgcgcagga tgtagctggc cgagctcttt ggaatctgca
     2821 acttacggct gatttcggcg ttcgacaggc cttccggctc ttgcgcgacg gcctccagca
     2881 tggccagcgc ccgttcgacc gcgaccgaag gggattcgtg gttccctggc
//
LOCUS       DASPUL010000571         4071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2029579, whole genome shotgun sequence.
ACCESSION   DASPUL010000571 DASPUL010000000
VERSION     DASPUL010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2029579"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VEJ00_13315"
     CDS             complement(<1..290)
                     /locus_tag="VEJ00_13315"
                     /inference="COORDINATES: protein motif:HMM:NF014857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase N-terminal
                     domain-containing protein"
                     /protein_id="HXX02190.1"
                     /translation="MKVLVLGGGGREHALVWKLRQSPRITQLYCTPGNGGIAEEAECL
                     PADLKSLDSITALAARLQPDLTLVGPELPLTLGVVDEFTRRGWAVFGPTAAAA"
     gene            490..1158
                     /locus_tag="VEJ00_13320"
     CDS             490..1158
                     /locus_tag="VEJ00_13320"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HXX02191.1"
                     /translation="MITVSKEDYLKSILEAESEGETVISARLADWHKVSPPAVTMALR
                     RLKKDRLVRVGSEGEVRLTAAGRKIARKLTLRHHLIERMLSEMFGMEWWKVHDEAERL
                     EHAVSPDFEAKLLAKLGSGGACPHGNLSEMEGSASRRKRGLVLLADAELDQTYNVSGI
                     YERDRRLLEFLEHRGVRPGAKLRLLERNYDQTLTLSTPAGTVSLGPAASVRVWLSPAN
                     RARH"
     gene            complement(1160..3883)
                     /locus_tag="VEJ00_13325"
     CDS             complement(1160..3883)
                     /locus_tag="VEJ00_13325"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXX02192.1"
                     /translation="MALNPGTRLGPYEIQSPLGAGGMGEVYRARDTRLERTVAVKILP
                     AHLSTDPEAKQRFEREARVISSLNHPHICTLHDVGNQDGIAYLVMECVEGESLDVRLQ
                     RGPLPLKQALECGMQICDALQRAHRAGIVHRDLKPGNIMLTASGAKLLDFGLAKPVAL
                     VASRAASHSTPLTPSTPTVNLSALSPAPGALTQQGSIVGTFQYIAPEALQGQEVDARS
                     DIFSFGCVLYEMVTGKRAFEGKSQISVASAILDKDPELVTKVQPMAPAALDHVVQRCL
                     AKEPEARWQSAADVAHELRWIASGGGSANVVPQEATRSSWRERVLWVAGVGALLIAVL
                     WLSLRGHEPERALRSYLPPPEDTGFDFTGDVSGPPAITADGTAIAFCARKQREQESVW
                     VQSLSDLAPRRLEGTDGAGFPFWSPDGKFLGFFADGHLKKVPAAGGPVTVLAEAPNAR
                     GGTWSQDNVIIYEPDYRDSLWRISAAGGAPTRLTRFDVSKHTTHRWPTFLPDGKHFLF
                     FATSHSGGSEQGVYFGSLADGSYKRVLDADSDAQYASGYLIYHLQSQLLAQKFDPANG
                     TVSGEPVTLANFVEYDAGTWHTTFAVSQNGLLVYEPGTKALGTDLLLLDRSGKILSNV
                     AERAQYKGSGRFSPDGKRLAIAIGDPEADIWVLDLNRGSRTRLTFGGGTHLNPSWSPD
                     GQRVVYVTQVGATLMAGTSLRARLASGGGQEEVLLPTNSSGNGSVQSPQFSPDGQYLI
                     HTEQSGPSDVAIWALPMSGDKKPFAVVQPPSAQARIVQFRLSPDGRWLAYSSTESGRE
                     QVYVTHFPSGQGRWQVSQAGGTYPCWRGDSKEIWFLGVDGAMHAAGVSASGDAFEVDP
                     ERLLFQLRYTAPLGEPYDLAPDAQRLIVTTLPESMPTPLVLVTNWTAEMKK"
BASE COUNT          802 a         1445 c         1096 g          728 t
ORIGIN      
        1 gctgccgcgg cagtcggccc aaaaacagcc catccccgcc gcgtgaactc gtccaccacg
       61 cccaaggtca aaggcagctc cggccccacc aaggtcaaat ccggctgcag tcgcgccgcc
      121 agcgctgtaa tcgagtccag actcttcaaa tccgccggca gacactcggc ctcttcggcg
      181 atcccgccat tccccggcgt gcaataaagc tgcgtgatgc gcggcgactg ccgcagcttc
      241 cacaccagcg cgtgctcgcg cccaccgcct ccaaggacga gaactttcat agaaaaatca
      301 ggttatgggg aaccggaaga cgctgtcaaa caggaaaacc aattcttgaa tcttgattgt
      361 tgattaacaa aagccgaagc gacgacacga tcaggtttga agcgaatcca tccctgccgt
      421 tcattcgaca atcaacaatc gccaatggac cagttaacca tacttaactc agccgtataa
      481 tctccctaga tgattacggt ctccaaagag gactacctca agtccattct cgaagccgaa
      541 agtgaagggg agactgtcat ttccgcccgc ctcgccgact ggcacaaggt gtctcctccg
      601 gccgtgacca tggctctccg ccgcctcaag aaagaccgcc tggtccgcgt cggctccgaa
      661 ggcgaagtcc gcctcaccgc cgccggccgc aagatcgccc gcaagctcac gctgcgtcat
      721 cacttgatcg agcgcatgtt gtcggaaatg ttcggcatgg agtggtggaa agttcacgac
      781 gaagccgaac gcctggaaca cgccgtctcc cctgatttcg aggcgaagct cctcgcgaaa
      841 ctcggctccg gaggcgcctg cccccacggc aacctcagcg agatggaagg ctcggccagc
      901 cgccgaaagc gcggccttgt cctgctcgcc gacgccgaac tcgaccagac ctacaacgta
      961 agcggaatct atgagcgcga ccgccgcctg ctggagttcc tcgagcaccg cggcgtccgc
     1021 cccggcgcga aactccgcct gctcgaacgc aactatgacc agactctcac gctctccacg
     1081 cccgccggaa ccgtctcgct cggccccgcc gcttccgttc gggtctggct gtctcccgct
     1141 aaccgcgccc ggcactgact tacttcttca tctctgccgt ccagttcgtc accagaacca
     1201 gcggcgtcgg catgctctcc ggaagtgtcg taacgatcag ccgctgtgcg tccggcgcaa
     1261 gatcgtaggg ctcccccaat ggcgccgtgt acctgagttg aaacagcagt cgctccggat
     1321 ccacctcaaa cgcatcgcca ctcgcactca ccccggcggc gtgcatcgcg ccgtccactc
     1381 ccagaaacca gatctccttg ctgtctccgc gccagcaggg atacgtgcct cccgcttggg
     1441 aaacctgcca tcggccctga ccgctgggaa agtgcgttac gtaaacctgt tcccgtcccg
     1501 actccgtcga gctgtacgcc agccatcgcc cgtcgggtga aagccggaac tgcacaatgc
     1561 gcgcctgcgc cgacggcggc tgcaccactg caaacggctt tttgtcgccg ctcatcggca
     1621 gcgcccaaat cgccacgtcc gagggacctg attgttccgt gtggatcaaa tactgcccat
     1681 ccggagagaa ctgcggcgat tgcaccgagc cattccccga ggaattagtc ggtaacagca
     1741 cttcctcctg cccaccccca ctggccagcc gtgcgcgcag cgacgttccc gccatcaagg
     1801 tagctccaac ctgggtcaca tagaccacgc gctgcccatc cggagaccac gacgggttca
     1861 gatgcgtgcc tccgccgaac gtcagccggg tgcgcgatcc gcgattcaaa tcgagcaccc
     1921 agatatcggc ctccggatct ccaatagcga tggccagccg ttttccatcc ggagaaaacc
     1981 tgccgctgcc cttgtattga gcccgctccg caacattgct caggatcttt ccgcttcggt
     2041 ccagcaacaa aagatccgtt cccagcgcct tcgtcccggg ctcatacacc agcaggccgt
     2101 tctggcttac cgcaaatgtc gtgtgccacg tgcccgcgtc gtactccacg aaattggcca
     2161 gtgttaccgg ctcgcccgaa accgtgccgt tcgcagggtc gaacttctgc gccagcaatt
     2221 gcgactgcag atggtaaatc agataccccg aggcatactg cgcgtccgaa tccgcgtcca
     2281 gcacgcgttt gtaagaacca tccgccagcg aaccgaaata cacgccctgc tcggagccgc
     2341 ccgagtgact cgtcgcgaag aacaggaaat gttttccgtc cggcaggaac gtcggccagc
     2401 gatgtgtcgt gtgcttgctc acatcgaaac gcgtgagccg cgtgggggcc cctccggccg
     2461 cgctgatacg ccacaacgag tcgcgatagt caggctcata gatgatgacg ttatcctggc
     2521 tccatgtccc tccgcgtgcg ttgggcgcct cggcgagcac cgtcaccgga cctcccgccg
     2581 caggaacctt cttcaaatgc ccgtcggcga agaatcctaa gaatttccca tccggcgacc
     2641 agaacgggaa cccagcgccg tcggtgccct ccagcctccg gggggcaaga tcgctgagcg
     2701 actggaccca gaccgactcc tgctccctct gcttgcgcgc gcagaacgca atcgccgttc
     2761 cgtcggccgt aatcgccgga ggacccgaaa cgtctccggt aaagtcgaag cccgtatcct
     2821 ccgggggcgg cagatatgat cgcagcgccc gctccggctc gtgtccgcgc aagcttagcc
     2881 acagaaccgc aatcagcagc gccccaaccc ccgctaccca caacacgcgc tcacgccaac
     2941 ttgatctggt cgcctcctgt ggaaccacat tcgcactacc gcccccactc gcgatccagc
     3001 gcagttcgtg tgcaacgtcc gcagcactct gccatcgtgc ttccggctct tttgccaggc
     3061 acctttgcac cacatgatcg agcgccgctg gggccatggg ctgcaccttc gtcaccagtt
     3121 ccggatcctt gtccagaatc gccgacgcaa ccgagatctg gctcttgccc tcgaacgccc
     3181 gcttacccgt caccatctcg tacagcacac agccaaagct gaagatgtcg ctccgcgcgt
     3241 ctacctcctg cccttgcagc gcttcgggcg cgatgtactg gaatgtgccc acaatggagc
     3301 cctgttgcgt cagagcaccg ggcgccgggc tgagtgcgga caggttcacc gtcggagtgg
     3361 atggcgtcaa aggcgtggaa tggctcgcag cccgcgacgc gaccaacgcc accggcttgg
     3421 ccagcccaaa gtcgagcagt ttcgctcccg aagccgtcag catgatattc cccggcttca
     3481 ggtcgcggtg cacaatgcct gcccggtgtg ctctctgcag cgcatcgcag atctgcattc
     3541 cgcattccag cgcctgcttc aggggcagcg gtcctcgctg cagccgcaca tccaggctct
     3601 cgccctccac gcactccatc accaggtagg caatcccatc ctggttgccc acgtcgtgca
     3661 gcgtgcaaat gtgcggatga ttcagcgacg agataacccg ggcttcgcgc tcaaaccgct
     3721 gcttggcctc aggatcagtg gatagatgag ccggcaggat tttgaccgcg accgtgcgct
     3781 cgagccgcgt atcccgcgcc cgatacacct cgcccatgcc gcccgcccca agcggcgact
     3841 gaatctcgta gggaccgagt ctcgtgccag ggttcagagc catcggtgcg cgccattata
     3901 tgccaataca gccgacaaac aggctgcgga aaacctctcg gtcgttcgcc gccaagcgcg
     3961 atctcagtcc caaccgtcct ctatggggta ccgtggaaga gcggcccttc agggccgcgt
     4021 aaatgcgttc ggagaagatt gggcttcagc ccctgtggaa tctgcgcggg g
//
LOCUS       DASPUL010000572         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1061382, whole genome shotgun sequence.
ACCESSION   DASPUL010000572 DASPUL010000000
VERSION     DASPUL010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1061382"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..934
                     /gene="fdxH"
                     /locus_tag="VEJ00_13330"
     CDS             41..934
                     /gene="fdxH"
                     /locus_tag="VEJ00_13330"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HXX02193.1"
                     /translation="MSDRRTLQIKQISGHSGAAPGLGVQRDYEVCKLIDTTICIGCKA
                     CEVACVEWNDMPFSSTTFDNTYQTMPETRWNFWNLIKFNEHQREDGTVQWLMRKDQCM
                     HCADPGCLRACPADGAIVQYTNGIVDFQQENCIGCEFCVSGCPYDIPKLNPATKKVYK
                     CTLCSDRVGQGLEPACIKACPTGCLKFGSKDDMKFIAEERARQLRENFGFAQAGVYDP
                     QSIGGTHVVYVLHDATDPERYGGLPKDPVIPWSYTIWKWLFKPVLGTLALFGGLGVLL
                     HYVTVGPRRPQPEPPIKEREG"
     gene            934..1677
                     /locus_tag="VEJ00_13335"
     CDS             934..1677
                     /locus_tag="VEJ00_13335"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HXX02194.1"
                     /translation="MSTAVERFDDKARGAFGKIGQTEVYEGELLRHRVYTRFLHWMVA
                     IFFFLALFSGFSFYLPWLFRWFTPIFGGGPLSREMHPWFGLGFVIFFGLQALNWIKPM
                     TWTAADTKWMRNLAKIIGGKEKMELPDTGFFNGGQKVQFWEIVGGCVVYLITGIILWI
                     GARTFGRWAIAVSYILHDISALIMLGGIFVHIYLSTIGEPGTFQAMIRGAVSEAWAWT
                     FHPAWYKEVTGRDPVQACEEARRRTGQAR"
     gene            complement(1740..2294)
                     /locus_tag="VEJ00_13340"
     CDS             complement(1740..2294)
                     /locus_tag="VEJ00_13340"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HXX02195.1"
                     /translation="MKSKIAVLLLAIVALTTASALAGDKNPVVGGQPMYPTKDIIDNA
                     VNSADHTTLVAAVKAAGLVDTLKGAGPFTVFAPTNEAFAKLPAGTVENLLKPENKDAL
                     TKILTYHVVPGRLSAADLRKQIKAGNGQAELKTVSGGTLTVMMQGKNIVLKDEKGGMS
                     TITIPNVFQSNGVIHVVDMVVLPN"
BASE COUNT          560 a          813 c          805 g          604 t
ORIGIN      
        1 ggcttgatcg cagcgctgaa gcgctgcgcc acccaaaatt atgagcgacc gcagaacact
       61 tcagatcaag cagatctcag gccactcggg cgctgcgccg ggtcttggcg tgcagcgcga
      121 ctacgaagtc tgcaagctga tcgacacgac aatctgcatc ggttgcaagg cgtgcgaggt
      181 cgcttgcgtc gagtggaacg acatgccgtt cagttcgacc accttcgaca acacgtacca
      241 gacgatgccg gagacgcgct ggaatttctg gaacctgatc aaattcaacg agcaccagcg
      301 cgaagacggc actgtgcaat ggcttatgcg caaggatcag tgcatgcact gcgccgatcc
      361 cggctgcctg cgtgcctgcc cggccgatgg cgccatcgtg caatacacga acggcatcgt
      421 cgacttccag caggaaaact gcatcggctg cgagttctgc gtttccggat gcccctatga
      481 cattccgaag ctcaatccgg cgacgaagaa ggtctataag tgcacgctct gttccgaccg
      541 tgtggggcaa ggcctggagc cggcttgcat caaggcttgc ccgacgggct gcctgaagtt
      601 tggtagcaag gatgacatga agttcattgc cgaggagcgg gccaggcaac tgcgcgagaa
      661 cttcgggttt gcgcaagccg gcgtatacga tccgcaatcg atcggcggca cgcacgtcgt
      721 ctacgttctg cacgatgcga ccgatcccga acgctacggg ggccttccga aagacccggt
      781 gattccctgg agctacacga tctggaagtg gctgttcaag ccggtgttgg gtactttggc
      841 gctcttcggc ggcctgggag ttctgctgca ctacgtgacc gtcggaccac gccgtccgca
      901 gcccgaacct ccgatcaagg aaagggaggg ctaatgtcaa ccgccgtcga acgtttcgat
      961 gacaaagcgc ggggtgcttt cgggaagatc ggccagaccg aggtctacga aggcgaactg
     1021 ctgcgccatc gtgtgtacac gcgcttcctg cactggatgg tcgctatctt ctttttcctg
     1081 gcgctgttct ccggattcag cttttatctg ccttggcttt tccgatggtt cactccgatt
     1141 ttcggaggcg gtccgctcag ccgcgagatg cacccctggt tcggtctcgg attcgtcatc
     1201 ttcttcggcc tgcaagctct gaactggatc aaaccgatga cgtggactgc ggccgacaca
     1261 aagtggatgc ggaatcttgc caaaatcatc ggcggcaaag aaaagatgga attgcccgac
     1321 accggcttct tcaacggcgg gcagaaagtg cagttctggg agatcgtcgg cggatgcgtg
     1381 gtctacctga tcacggggat cattctgtgg atcggcgcac gcaccttcgg acgctgggcc
     1441 attgccgtca gctacatctt gcacgacatc tcagcgctca tcatgctggg cggaatcttc
     1501 gttcacatct acctcagcac gatcggcgag cccggcacgt ttcaggcgat gattcgcgga
     1561 gcggtcagcg aggcctgggc ctggacgttc catcccgcgt ggtacaagga agtgaccggc
     1621 cgcgatcctg ttcaagcctg tgaggaagcc cggcgcagga ccgggcaggc cagataatca
     1681 ggcaatcaat caaaaagggc gctcctcacc ggagcgccca gcaaacatct gacaaacgac
     1741 taattcggca gtacgaccat atccacgacg tggataaccc cattcgactg aaaaacgttc
     1801 gggatcgtga tggtcgacat gccgcccttt tcgtccttta agacgatgtt cttgccctgc
     1861 atcatgacgg tcaacgtgcc tccactcacc gttttgagtt ccgcctgccc gttgccggcc
     1921 ttgatctgct tccttagatc agctgcgctc agacggcctg gaactacgtg gtaagtaagg
     1981 atcttcgtca gggcgtcctt gttctcgggc ttgagcaggt tttcgactgt gccggcgggc
     2041 aatttcgcga aagcctcgtt cgttggcgca aagacggtga acggccccgc gcctttaagg
     2101 gtgtctacga ggccggcagc cttcactgca gctacaagcg tagtgtggtc ggccgaattg
     2161 accgcgttgt cgatgatgtc tttcgtgggg tacatgggct gaccgccgac gaccggattc
     2221 ttatctcccg caagcgctga cgccgtcgta agcgccacga ttgccagtag cagaactgca
     2281 attttgctct tcatgatctc tcctgaaaac gatctctcct ggaattgaac tgctttcatg
     2341 acaggtacgc gggcttcgtg ctcggcggat tgcaagctcg gttttttcgc cccgttctgt
     2401 cgaaagagtc acaacgcgct ggctgtgctg aaccggatat acgcagcctg ggagtttttg
     2461 gatttactgc ggctgagaaa aaacttcttc atgaacgctg cgtgaaaaac tcgcagtcca
     2521 gccaatccag aactggtttc gtcgcgtatt cccattcgta gacggagcca atcgtaaccg
     2581 ctgctgcgtg gcgccgcgtg gcagcgggca gtacccgcga gggccgaagt ccacaattcc
     2641 aactttcaag aggtacacaa tgaagctgct gaaatctcca gtggccttcg ctctggcgct
     2701 gctggcgctc attccggcca gtctgttcgc gtccagccat cgcgaggccc ccatctctgc
     2761 gctcgatcat tccgcggatg tt
//
LOCUS       DASPUL010000573         5964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1333439, whole genome shotgun sequence.
ACCESSION   DASPUL010000573 DASPUL010000000
VERSION     DASPUL010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333439"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="VEJ00_13345"
     CDS             complement(<1..1541)
                     /locus_tag="VEJ00_13345"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXX02196.1"
                     /translation="MAIKIKIPKENGADGKRTRGLPRDPLLRAALVIFLILVVGFSGF
                     FSYFYIKYDRVIERRFRTPVFSNSAKIYALPETVRTGERIEVRDIAAQLRRAGYTDQG
                     HPDKTGESQLGSFKLVSDGIEITPGQESYHSPEAARISVQDGQVERITSKGIDLSAYE
                     LEPQLVTALFDAEQRSKRELVKYNDIPPVVVQAVLAIEDRRFFEHSGVNFLRMAEAAW
                     IDVVRGRHEQGGSTITMQLSRGFFLTPEKTVKRKLTEMLIAEELEQKFSKQQIFEFYA
                     NWVPLGQRGSFAISGLAEASQAYFNKDLKDLTLPEAALLAGLIQRPSYLSPYRHPERA
                     LERRNLVLESMVETHAITREQADKAKATQLKLAPPNVEASDAPYFVDLVRDQLISKFS
                     ESDLNEQSYRIYTTLDPDLQKAAAQSVEMGIKLVDEQVKKMRTKRVKVGKKFETKVEQ
                     GPQAQVALVALDPHTGAVLALVGGRDYGWSQLNHAVAKRPTGSIFKPFVYAAAMNTAL
                     DGSQTV"
     gene            complement(1920..3194)
                     /locus_tag="VEJ00_13350"
     CDS             complement(1920..3194)
                     /locus_tag="VEJ00_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02197.1"
                     /translation="MKRKFQVLVGLTVVVLVALWLVGCDHYVCSSGATFGNSSCTASG
                     SGLGTNTGGVGNSLAFAVDQQGTVDGFTLNTSASTFGLISNFSAPTIPQLDFGVGMVA
                     AQKKFLYAGFGSSTPKELFGWSINSSTGALTPVSGSPYATPALGSVAATGFPQQSMIT
                     NPAGTLLFIAEASTDSIAIYQINGDGSLTAQAAFSTFPFQPVNMTTDGLGKYLYVTDV
                     ISSHEGTGGIAAYSIGSSGALTPVAGSPFVLTTPMWQLQGEASGKYLVGTSGKTAALP
                     GGTDDDNLYVFSINQSTGALTLNGTTPTQNSPFGIAVSPVATNGGTFVYSFSVNDTGT
                     GFNPIEGYSLSSTGALTSVGVFSNVQLGEWGQFDQSGTTLLDYAEIQNLGTGTITTQL
                     GAFSVASSGAITQIGSPVTLGTPGYWVVTDTH"
     gene            complement(3504..3863)
                     /locus_tag="VEJ00_13355"
     CDS             complement(3504..3863)
                     /locus_tag="VEJ00_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02198.1"
                     /translation="MGKVKLILVFGLLALLLSTGWQIAACELADYELRDDLKDLASLS
                     GARIGLTAPKSDDDLRDEVISKAHEHDIVLQREQITVRRSGPADAPLVYLAARYSQRV
                     VLPGFKLTLHFAPNSKR"
     gene            complement(3929..4285)
                     /locus_tag="VEJ00_13360"
     CDS             complement(3929..4285)
                     /locus_tag="VEJ00_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02199.1"
                     /translation="MGTVKAIVGILAIVAVIYCGFQLIPPELANYSFNDDLRNIAMVG
                     GSNPRQTDQDLIEAVMKKAQEHDIPLTPEHVVVQRIGTPGAPAVYVAADYSVPVNLPG
                     YSFTMHFTPNSGNKGF"
     gene            complement(4336..>5964)
                     /locus_tag="VEJ00_13365"
     CDS             complement(4336..>5964)
                     /locus_tag="VEJ00_13365"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HXX02200.1"
                     /translation="GNRSFNKIVAIVREEMDRVGQEFYLPALHPREIWEASGRWTVMG
                     DNLFRLKDRKGTDMCLGMTEEEVMSSIALKDLRSYKQLPQIWYQIAPKFRDEPRPRSG
                     LLRVRQFIMKDSYSFDIDAAGLDVSYRKHYDAYCRIFDRCGLKYMVVEADSGAMGGKE
                     SHEFMVRTPAGEDQIVSCDGCNYAANLEKATSKLDTVEDLKPEGDGKPLPVHTPGQKT
                     IEEVARFLGVSPKNKIKTLALMADEVDEKGKKKSRAIVVLMRGDHQLNEAKVNGAVGA
                     ATRAMEESEIEGLFNSPAGYLGPVGIEWARDIKNHEGKPVLLVDKALEGRANLIGGAN
                     KRDYHLKNLTPGKDFQPTAYADLRAVTAGEACPNCGKPLRIDTAVEIGHIFKLGYKYS
                     ETMGTRVLDKNGKEVMPIMGSYGIGIERILTAAIEQGNDADGFWLPTAIAPFEIVVAP
                     VNMKDEPVRAAAEDIAKRLKAAGFDVILDDRDERPGVKFKDADLVGIPFRVTVGKKVT
                     EGTVEVVIRSTRQVCDVTISAIVEHFQGLLRRAA"
BASE COUNT         1113 a         1929 c         1680 g         1242 t
ORIGIN      
        1 acggtctgcg aaccgtcgag cgccgtattc atcgccgccg catacacgaa cggcttgaaa
       61 atcgatcccg tcggccgctt cgccaccgca tgattcagct ggctccagcc gtagtcgcgg
      121 ccgcccacca gcgccaggac cgccccagtg tgcggatcga gcgccaccaa cgccacctgc
      181 gcctgcgggc cttgctccac cttggtctcg aacttcttac cgactttcac gcgcttggtg
      241 cgcatcttct tcacttgctc gtcgaccagc ttgattccca tctccaccga ctgtgccgcc
      301 gctttctgca gatcgggatc tagcgtggtg tagatgcgat acgactgctc gttcagatcg
      361 ctttcgctga acttgctgat cagctgatcg cgcaccaggt cgacaaaata tggcgcgtcg
      421 ctggcttcca cgttcggcgg cgccagcttc agctgtgtcg ctttcgcctt gtcggcctgc
      481 tcgcgcgtga tggcgtgggt ctcgaccatt gactccagca ccaggttccg ccgctccagg
      541 gcgcgctccg gatggcggta cggcgaaaga taactcggcc tctggatcaa acctgccagt
      601 agcgcggctt cgggcaacgt cagatctttc aggtccttgt tgaaataggc ctgcgacgcc
      661 tccgccagcc cgctgatggc gaacgatcca cgctggccca gcggcaccca gttggcatag
      721 aactcgaaaa tctgctgctt cgagaacttc tgctccagtt cctcggcaat cagcatctcg
      781 gtcagcttac gcttgaccgt cttctcggga gtcaggaaga atccgcgtga cagctgcatg
      841 gtgatggtcg atccaccctg ttcatgccgc ccgcgcacca catcgatcca cgccgcctcc
      901 gccatgcgca gaaaattcac cccgctgtgc tcgaagaagc gccggtcttc gatcgcgagc
      961 actgcctgca ccacgactgg aggaatgtcg ttgtacttca ccagctcgcg cttcgagcgc
     1021 tgctcggcat cgaacagcgc cgtcaccagc tgcggctcga gctcatacgc cgacaaatcg
     1081 atgcccttgc tggtgatgcg ctccacctgc ccgtcctgga cgctgatccg cgccgcctcc
     1141 gggctgtggt aggactcctg ccccggggtg atctcgattc cgtcgctcac gagcttgaag
     1201 ctgcctagct gtgactcgcc ggttttgtcc gggtgcccct gatccgtata cccggcccgc
     1261 cgcagctgcg ctgcgatgtc gcgcacctcg atccgttccc cggtgcggac ggtctcgggc
     1321 agggcgtaga tttttgccga attgctgaac accggcgtgc ggaagcgccg ttcgatgacg
     1381 cggtcgtact tgatgtagaa gtaggaaaag aacccgctga aacccactac caggatgaga
     1441 aatatgacca gcgccgcccg caaaagcggg tcgcgcggca gcccgcgcgt gcgcttaccg
     1501 tccgcgccgt tttccttcgg aatcttgatt ttgatggcca cagttctatg tcagcctcta
     1561 gcggtgaatc accggcactg gcggcgcaga cgaagggata ctcctattgt accctcccct
     1621 gaaagccaag agacccgcag ccggcgcggg tctctcagat gtcaacaagc gccctttcgt
     1681 tgcccccttg cggttcgtcg gtcgcgccgt agccgttgtc cgagaacgcg accgagggta
     1741 gcgtgccgct tgatgctcgc gttgaccgcc aatggaaaga gccagaagtt gttgagatcg
     1801 gcctcctcca tgacggcgac ccggaggtgc ggaaacgaaa agagacccgc tgtggatgcg
     1861 ggtctctgtg ctgttaacag gtggggccgt cctccgaacg tgagctcctc cggtgcgatc
     1921 taatgagtgt ccgtcaccac ccagtagccc ggggtcccca aggtcacagg agaaccaatc
     1981 tgagtaatcg ctccgcttga tgcgacacta aaggccccca gctgggtggt gatcgtgccg
     2041 gtgcctaggt tctgaatctc ggcgtaatca agaagcgtag ttccgctttg atcaaattgt
     2101 ccccattcgc ccagctgtac gttgctgaac acgccgaccg acgtgagcgc tccggtggag
     2161 ctgagggagt agccttcgat cgggttgaaa cccgttccgg tgtcgttaac actgaaggag
     2221 taaacgaacg ttcccccgtt tgtggcgacg ggcgacaccg cgatgccgaa gggggaattt
     2281 tgagtggggg tcgtgccgtt cagcgtcagc gctcctgtgc tctggttgat gctaaacaca
     2341 tacaggttgt cgtcgtccgt ccccccgggt aaggcggctg tcttcccact ggtcccgacc
     2401 agatactttc cactcgcctc gccctggagc tgccacatgg gagtggtcaa aacgaacggg
     2461 ctacccgcca cgggggtgag tgcgccgctg ctgccgatcg aataggctgc aatgccgccg
     2521 gttccttcgt gcgagctgat cacatcggtc acgtacagat acttgcccag gccgtcggtg
     2581 gtcatgttca cgggctggaa tgggaacgtg ctaaaagccg cctgcgccgt taaggatccg
     2641 tcaccattaa tttggtaaat cgcgatcgag tcggtgctcg cttcggcgat aaacaacagc
     2701 gtcccggcag ggttggtgat catgctttgc tgggggaacc cggtggcagc aacgcttccc
     2761 agggccggcg tagcgtaagg actgccgctg accggcgtca gcgcgcctgt gctgctgtta
     2821 atggaccagc cgaagagctc ttttggagtg gaagaaccga atcccgcgta cagaaatttc
     2881 ttctgcgccg ctaccatgcc tacgccgaaa tcgagctgag ggatggtggg cgcggagaaa
     2941 ttcgatatga ggccaaacgt gcttgccgac gtgttcagcg taaacccatc gacggtgccc
     3001 tgctggtcga cggcaaaagc aagcgagttc cccacgccgc cggtattcgt gcccagcccg
     3061 ctgcccgatg ccgtgcaact gctgttgcca aatgtcgcgc cggaactgca gacgtagtgg
     3121 tcgcatccca ccagccacag ggccaccagt accactaccg tcagaccaac cagcacctga
     3181 aatttcctct tcatgaaccg cctccgcctg aactttctcg agctcgcccc agtttcccga
     3241 gattcgccga attatctctg tgcttcgcct aactttcttc ggggcttcgc cgggcttcga
     3301 caatctctat taccgcgccc gcagaagcac gattgggaaa agacttaccc cttggcgata
     3361 gtagggtcga gctggtcaca ctgacaacgt tacgaacgtc acgggacgca gtggcgaaga
     3421 aactttggtc tcttttccat ctgcactcaa ttcaacaagt cccaatggga aggaagcacg
     3481 aaagaaaaca aggagggaac agattaccgc ttgctgttgg gcgcaaaatg gagagtcagc
     3541 ttgaatccag gaagcaccac acgctgactg tagcgcgcgg ccagatatac cagcggagcg
     3601 tccgcgggtc ctgatcgccg caccgtgatc tgctcgcgct gcagcacgat gtcgtgctcg
     3661 tgcgccttgc tgatgacctc gtccctgaga tcatcgtcgg acttgggagc agtcaagccg
     3721 attcttgcgc cgctcagcga cgccagatcc ttcagatcgt cccgcagttc gtagtcagcc
     3781 agctcgcagg cggcgatttg ccagcccgtg ctgagcaaca aggcaagcag gccgaagacg
     3841 aggatgagct tcacttttcc cacgaacgaa atccaccggg aacgtgcgtt gggcgggaac
     3901 tgccagacag ccgcatttcc cgtgccgtct agaagccctt gttgccgctg ttgggcgtga
     3961 aatgcatggt gaaggaatat cccggaaggt ttaccggcac gctgtagtca gccgcgacgt
     4021 agaccgccgg cgcgcccggc gtgccaatgc gctgcaccac gacgtgctcc ggcgtgagcg
     4081 ggatgtcgtg ctcctgcgcc ttcttcatca ccgcctcgat caaatcctgg tcggtctggc
     4141 gcgggttgga gccgcctacc atcgcgatat tccgcagatc gtcattaaag gaatagttcg
     4201 cgagctcagg cggaatgagc tgaaatccgc agtagataac ggccacgatg gccagaatcc
     4261 cgacgatggc tttgactgta cccatgatcc ccatccgctc tgccggggac cggaagcggc
     4321 ctcaatcggc cggcttcatg ccgcgcggcg gagcaatccc tgaaaatgct ccacgatcgc
     4381 ggaaatcgta acatcgcaca cctgtcgagt cgaacgaata acgacctcga cagtaccttc
     4441 ggtaaccttc ttgccgaccg taacccgaaa cgggatgccg accagatcgg catccttgaa
     4501 cttcaccccc ggccgctcgt cccggtcgtc gagaatcacg tcaaaccccg ccgccttcag
     4561 acgtttggcg atgtcctcgg ccgccgcccg caccggttca tccttcatat tgaccggtgc
     4621 gaccacgatc tcaaacggcg caatcgccgt cggcagccag aatccgtccg catcattgcc
     4681 ctgctcgatc gcagccgtca ggatgcgctc gattccgatg ccgtagctgc ccatgatcgg
     4741 catgacttcc ttgccgttct tgtcgagcac ccgcgttccc atcgtctcgg agtatttgta
     4801 gcccagcttg aaaatgtgcc cgatctcaac cgccgtgtcg atgcgcagcg gcttgccgca
     4861 gttggggcaa gcctcaccgg cagtcaccgc ccgcagatcg gcgtacgccg taggctgaaa
     4921 gtccttcccc ggcgtgagat tcttcaggtg atagtccctc ttgttcgcgc cgccgataag
     4981 gttcgcgcgc ccctcgagcg ccttgtccac cagcagcacc ggcttgcctt cgtgattctt
     5041 gatatcgcga gcccactcga tgccgaccgg gcccagatat ccagcagggg aattgaacag
     5101 gccttcgatc tcgctctctt ccatcgcccg cgtcgccgcc ccgactgctc cgttgacctt
     5161 agcctcgttc agctgatgat ccccccgcat cagcaccacg attgcgcggg actttttctt
     5221 tcccttctcg tcgacctcgt cggccattag cgccagcgtc ttgatcttgt tcttcgggga
     5281 gacgcccagg aagcgcgcta cttcttcaat cgtcttctgt cccggtgtat gcaccggcag
     5341 aggcttgcca tcgccctccg gcttcaagtc ttccaccgtg tcaagcttcg aagtagcctt
     5401 ctccaggttc gctgcgtagt tgcacccgtc gcagctgaca atctgatctt cgcccgccgg
     5461 agtccgcacc atgaactcgt gggactcctt tccgcccatc gccccggagt cggcttcgac
     5521 caccatgtac ttcagcccgc agcggtcgaa gatgcggcag taggcgtcgt agtgcttgcg
     5581 atacgacaca tccagtcccg cggcatctat gtcgaacgaa tacgagtcct tcatgatgaa
     5641 ctgccgcacg cgcagcaggc cggatcgcgg ccgcggctcg tcgcgaaact ttggcgcgat
     5701 ctgataccag atctgcggca gctgcttgta gctgcgcagg tctttcaagg cgatcgagga
     5761 catgacttct tcttcggtca tgcccaggca catgtcggtg cccttgcggt ctttcaaccg
     5821 gaagaggttg tcgcccatca ccgtccagcg cccgctcgct tcccagattt cccgaggatg
     5881 cagggccggc aaatagaatt cctgcccgac ccggtccatc tcttcgcgca cgatggccac
     5941 gatcttgtta aacgaccggt tgcc
//
LOCUS       DASPUL010000574         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1608537, whole genome shotgun sequence.
ACCESSION   DASPUL010000574 DASPUL010000000
VERSION     DASPUL010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608537"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1627)
                     /gene="hpnE"
                     /locus_tag="VEJ00_13370"
     CDS             complement(209..1627)
                     /gene="hpnE"
                     /locus_tag="VEJ00_13370"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HXX02201.1"
                     /translation="MTESSPTVAIAGGGLAGLAAACALADTGFRVTLFEKRPFLGGRA
                     SSWEHPGTGEVVDNCQHVLFRLCTNLMEFYQRIGVASQIRWYDEMTFIEPGGRASRME
                     SSFLPAPFQTAPSFLRFSFLDAADKLSIARALVPITLTGQRDNGQSFQQWLDRHSQTK
                     RAVERFWRPILVSALSEDLDRISVSAAAQVVRTSMLSPEARQMGVPTVPLTDLYNAAG
                     NYIRAHGGVIQFRRALEAFSPGASRVEARTAGQETGEAESFDYLVVALPFDALDRVLP
                     DAPESVPMREKISHFENSPITGIHLWFDRQISELDHAVLLDRTIQWMFHKSRLQPMRS
                     QSGNGAPEGSYIELVVSSSKSLIDKSRSEIVDLALAEVREFFPAAREATLVKSTVIKE
                     VNATYSPRPGIDAHRPSANTEWPRVFLAGDWTATGWPATMEGAVRSGYLAAEALARGS
                     GRKDAKFLSPDLPPTGFMRLFA"
     gene            complement(1683..>2071)
                     /locus_tag="VEJ00_13375"
     CDS             complement(1683..>2071)
                     /locus_tag="VEJ00_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HXX02202.1"
                     /translation="CGLAFQLTNIIRDVKEDASMGRVYLPEEDLVKFGLSAAELLAKP
                     DPVRFRPLLALEADRARELYAAGAELIPYVSEDSQPALWVLVTIYRKLLEKIAAKQYD
                     VFTAKVALSRWEKLRVLGKGFFKRIT"
BASE COUNT          411 a          623 c          642 g          395 t
ORIGIN      
        1 ttcaaagacg gcaagcccgc ctggtaggat cttcgctttt cttcgcgtcc tttgcggctt
       61 ttctttgcga tctctgcgct caaaggcttt ttaccgcgga gtcgcgaagg aaaggcccgc
      121 ggtgagcgca aagaaaacgc attttcagga gcgtgcggtg aaggtcaaag tcttgtaagt
      181 ccggatttcg cgattctggg ttgcggattc aggcgaagag ccgcatgaat ccggtcgggg
      241 gtaggtccgg tgataagaac ttggcatcct tgcgcccgga gccgcgcgcc agcgcttctg
      301 ccgccaggta tccgctgcgg accgcgcctt ccatggttgc tggccagccg gtcgcggtcc
      361 agtcaccggc gagaaaaacg cgcggccatt ccgtgtttgc cgatggacgg tgcgcgtcga
      421 ttcccgggcg cggcgagtag gttgcgttca cttccttgat cacggtggac ttcaccaaag
      481 tcgcctcacg cgcggcaggg aagaactcgc gcacttcggc gagagcgagg tcgacaattt
      541 cggatcgcga cttgtcgatc agagacttcg acgaacttac aaccagttcg atgtagctgc
      601 cctccggcgc tccgttcccg gactggctgc gcatcggctg caggcgcgat ttgtggaaca
      661 tccactggat ggtgcggtcg agcagaacgg cgtgatcgag ttcggaaatc tgccgatcga
      721 accacaaatg gatgcccgtg atcggcgagt tctcgaaatg agagatcttc tcgcgcatgg
      781 gaacactctc cggagcatcc ggcaggacgc ggtcgagagc gtcgaagggc agggcaacca
      841 ccaggtagtc gaaggactcc gcttctcctg tttcctgccc tgctgtgcgc gcctcgaccc
      901 gcgacgcacc aggactgaag gcttcgagcg ctcgccggaa ctgaatcacc cctccatgag
      961 ctcgaatgta gttaccggcc gcgttgtaga gatcggtcag gggaaccgtt ggaacgccca
     1021 tctggcgggc ttccggagac agcatcgaag tgcgcaccac ctgcgccgcc gcggaaacgg
     1081 aaatccggtc aaggtcctcg ctcagcgcgc tgaccagaat cggcctccag aagcgctcga
     1141 cggcacgttt agtttggctg tggcgatcaa gccattgctg aaacgattgc ccgttgtcgc
     1201 gctgcccggt cagcgtaatc gggaccagcg cacgcgcgat gctgagcttg tcggccgcat
     1261 ccaggaacga aaagcgcaga aatgatggtg cggtctggaa gggcgccggg agaaacgacg
     1321 actccatccg tgaagcccgc ccgcccggct cgatgaacgt catctcgtcg taccagcgga
     1381 tttggctggc tactccgatg cgctggtaga actccatcag attcgtgcac aggcggaaga
     1441 gcacatgctg gcagttgtcg accacctcgc ccgtgcccgg gtgctcccac gacgaagccc
     1501 ggccgcccag gaacggacgc ttctcgaaca acgtcacgcg aaaacctgtg tcggcaaggg
     1561 cacaggccgc agccagtccg gccagtccgc ctccggcgat ggccacagtg ggagaagatt
     1621 ctgtcatgaa gcgattgcag attgcagatt gtagattgaa gagtccacgg gaaattgaat
     1681 gatcaggtta tgcgcttgaa gaagcctttc cccagtacgc gcagtttctc ccaacggctc
     1741 agagccactt tggcggtgaa cacgtcgtac tgcttggcag ctattttctc gagcagcttc
     1801 cggtagatcg tgaccagcac ccacagcgcc ggctggctgt cttccgagac ataaggaatg
     1861 agttccgcgc ccgccgcgta gagttcgcgc gcgcggtcgg cttcgagcgc cagcaacggc
     1921 cgaaaacgaa ccggatccgg cttggccagc agctccgcgg ccgagaggcc gaacttcacg
     1981 agatcctctt cgggaagata tacccggccc atgctcgcgt cttccttcac atcgcggatg
     2041 atgttggtga gctggaaggc cagcccgcag c
//
LOCUS       DASPUL010000575         4923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_380567, whole genome shotgun sequence.
ACCESSION   DASPUL010000575 DASPUL010000000
VERSION     DASPUL010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380567"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VEJ00_13380"
     CDS             <1..519
                     /locus_tag="VEJ00_13380"
                     /inference="COORDINATES: protein motif:HMM:NF024674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HXX02203.1"
                     /translation="VLRPANFPADMPGWGLYEGSKGKIAYAVINLQGRVFMGSNDDPF
                     RMADQLLKKVEAKVVLVDFHAEATSEKISMGWYLDGRVTAVIGTHTHVPTADERVLPN
                     GTAYITDVGMTGPYDGVIGVKKELVVGKFLNGMPVRFEPATGDVRLCSVVIDCDEKTG
                     KAREIERLMLSQ"
     gene            710..2590
                     /locus_tag="VEJ00_13385"
     CDS             710..2590
                     /locus_tag="VEJ00_13385"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02204.1"
                     /translation="MRRAATIFAAGLLTAIVAAPALADDQSAAPSASPAPPPVISCGN
                     GIPGGIMCFPTKEDIKEARTAFALGVKLQDHNRLEEAMDKFDHAARLNPQEVKFLTAR
                     EMVKAQLVFNHVERGNVLMAEDLRPEAVKEFRAALALDPEDEFARERLEDADAEIAEQ
                     QAAALPGPLAASGEIHLEPRYALATFHYTGDVKSLFIEMGAAYGLSVIFDDSVKDRPV
                     RFNIDDADFYTALKLATEVTKTMWAPLSARQMLIADETQENHKQFDRMALRTFVLPAH
                     SSPQEATEISNALRNVFELKSVTLGQTAGTVEVKGPQPVLDACARFMQQLDRDRPQVM
                     LDVRVFQIDHQLTRDMGLHIPYTFNLFNIPATALAGLGGQNIQQLINQLISSGGINQA
                     GSSALSGLLAQLGSQSNSIFSQPLATFGGGLTFEGLSLDQLVAKLSLNESWVRSLQSL
                     MLRATQGNDAILHSGTRYPIQNASYAPIFNSPQIAHVLGNQSYVPPFPSVSYEDLGLN
                     MKVKPVIHADNSVSLQIELQVRALTGQSANGVPVISNQEYKGSIRLENGEPAVVAGQV
                     SYSETLAIGGLPGLAYIPILNHSAVTNTKQLDANELMIVITPHVVSNFDRKNSEIWIS
                     QN"
     gene            complement(2739..3638)
                     /locus_tag="VEJ00_13390"
     CDS             complement(2739..3638)
                     /locus_tag="VEJ00_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXX02205.1"
                     /translation="MRSLTKTILILVLSGATAGAQSSAAKPDPKADKAPANVNLPSEA
                     TVESFLQQQFGYQEGLSWKLASIKPAGIAGLAEVTAVMANQEGQQVFHFYVAPDGEHA
                     LVGDVIPFGARPFDPVKKTLDKGITGPDRGPKNSPVTIVEFGDLQCPACKAAQPTIEA
                     LIAVEPTAHFVFQNFPLEMHNWAAKGAAYADCVGRASNDAFWKFISKTYETQADITAA
                     TADEKLTAIADQAGVKGSDIAACAAQPDTKARVDASIALGKSVDVTGTPTLFINGRKI
                     GSFDARLSDVYKSLVDFAAKTAN"
     gene            complement(3715..>4923)
                     /locus_tag="VEJ00_13395"
     CDS             complement(3715..>4923)
                     /locus_tag="VEJ00_13395"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HXX02206.1"
                     /translation="LTICEDAWNDKQFWRKRLYTVDPVESLIHSGGNFVLNISASPFW
                     IGKGAFRREMLASIAAQHKVPVVMVNQVGGNDSLVFDGSSLVLNREGKVVAQGRSFEE
                     DLVFFDSETLTGEMHEQIEGDEASAYAALVLGTRDYMHKCGFQKAIIGLSGGIDSALT
                     AVIAADAVGPENVIGVDIPGPYSSQGSIDDARALAKNLGIRFELLSINPAYGAYHQTL
                     QGVFSGQKEDVTEENIQSRARGTLLMALSNKFGAIVLSTGNKSELGVGYCTLYGDMVG
                     GLAVISDVPKTLVYRLSHYVNSRRPVIPRATLEKAPSAELRPNQKDSDSLPPYDILDA
                     VLEDYVEDSHPAERIAADHGFDIEVVRRVIRMVDRAEYKRQQAAPGLKISPKAFGYGR
                     RIPIAAKCEV"
BASE COUNT          994 a         1527 c         1417 g          985 t
ORIGIN      
        1 gtcctgcgcc ccgcgaactt ccccgccgac atgccggggt gggggcttta tgaagggtcg
       61 aagggcaaga ttgcatacgc cgtcatcaac ctgcagggac gcgtcttcat gggttccaat
      121 gatgatccgt tccggatggc cgaccagctc ttgaagaagg tcgaagccaa ggtagttttg
      181 gtcgatttcc acgccgaagc gacctccgaa aaaatctcca tgggctggta tctcgatggc
      241 cgggtcacgg ccgtgattgg cacgcacacg cacgtgccca ccgccgatga gcgcgtgctg
      301 cccaacggca ccgcctacat aacggacgtc ggcatgaccg gtccatacga cggcgtgatc
      361 ggggtcaaga aagagctggt ggtgggcaag ttcctcaacg gcatgccggt gcgctttgag
      421 cccgcgaccg gcgacgtgcg cctgtgctcg gtagtgatcg actgcgacga aaagacgggc
      481 aaggcgcggg aaatcgaacg cctcatgttg tcgcagtaac ctccttcaca agccctcatc
      541 cccagaccgc gaagctgaat ctctgcgctc cagctgcaag ctcccgcacg gcatgcgttc
      601 ttcttgggta agtgcctgta tcttcagttg ttgaacccga gggtacaagc gcccttcgga
      661 gcgctgattc caatatcaaa tcgccgggag ccaagttaaa atcataggga tgcggcgggc
      721 agcaaccatc ttcgcggcgg ggctgttgac ggccattgtg gccgcgcctg cgctggccga
      781 cgatcagtct gctgcgcctt ccgcttctcc ggctccccct cccgtcatct cgtgtggcaa
      841 cgggattccc ggcgggatca tgtgctttcc taccaaagaa gacatcaagg aagcccgcac
      901 cgctttcgcc ctgggtgtga agctgcagga tcacaaccgc ctcgaagaag cgatggacaa
      961 gtttgaccac gcggcgcgcc tgaatccgca ggaagtgaag ttcctgaccg cccgcgagat
     1021 ggtgaaagca caactcgtgt ttaatcacgt agagcgcgga aatgtcctga tggccgagga
     1081 ccttcgccct gaagccgtga aggagttccg agccgcgctc gcgcttgacc cggaggacga
     1141 gttcgcccgg gagcgcctgg aagatgctga tgccgagatc gccgaacagc aggctgccgc
     1201 tttgcccggc ccgcttgcgg catcaggcga aatccatctg gagccgcgct acgccctggc
     1261 cacgttccac tacaccggcg atgttaaaag tctgttcatc gaaatgggcg cggcctacgg
     1321 gctttccgtc atcttcgacg attcggtcaa ggaccggccg gtgcgcttca acatcgacga
     1381 cgccgatttc tacaccgcgc tgaagctcgc gaccgaagtt accaagacca tgtgggcgcc
     1441 gctctccgcc cggcagatgc tgatcgccga tgagacgcag gaaaaccaca agcagttcga
     1501 tcgcatggcg ctgcgcacct tcgttcttcc ggcgcactcc agtccgcagg aggcgaccga
     1561 gatcagcaat gctctgcgca acgtgttcga gttgaagtcc gtcactttgg gccagaccgc
     1621 aggcaccgtg gaagtgaagg gcccgcagcc ggtactcgat gcctgcgccc gcttcatgca
     1681 gcagttggac cgcgatcgcc cccaggtgat gctcgacgtc cgcgtcttcc agattgacca
     1741 ccagctcacc cgcgacatgg gtttgcatat cccctacacc ttcaacctgt ttaatattcc
     1801 ggccacagct ctagccgggc tggggggaca gaatattcag cagctgatca accagctgat
     1861 ttcttcgggg ggcatcaacc aggccggaag tagcgcgctc tccgggcttt tggcccagct
     1921 cgggagccag tccaattcca ttttcagcca gccgctggcg actttcggcg gaggccttac
     1981 cttcgaagga ctgagcctcg accagcttgt agccaaactc tcgctcaacg aatcgtgggt
     2041 gcgcagcctg caaagcctca tgctgcgcgc cacccaggga aatgacgcaa tcctccactc
     2101 aggaacgcgt tatccgatcc agaacgccag ctacgcgccc atcttcaact ccccccagat
     2161 tgcccacgtc ctggggaacc agagctatgt tccgccgttt ccttccgtca gctatgagga
     2221 cctgggcttg aacatgaaag tgaagccggt cattcacgcc gacaattccg tcagcctgca
     2281 aattgagttg caggtccgcg cgctcaccgg ccaatctgcc aacggagttc cggtgatctc
     2341 gaatcaagaa tacaagggca gcatccgcct ggaaaacggg gagccggccg tggtggcggg
     2401 ccaggtatcg tacagcgaga cgctggccat aggcggcctg cctggattag cgtacatccc
     2461 catcttgaac cactccgcgg tgaccaacac caagcaactg gacgcgaacg aactgatgat
     2521 cgtcatcacc ccgcacgtcg tgtccaattt cgaccggaaa aactcggaaa tctggataag
     2581 ccaaaactga aaagctaggg gccaggggtc aggggccagg gatcagtggt ggaaattgat
     2641 atcgcctgcg gcctgcaacc gacggcgtct tagacaaaac atggcccgaa gcggatttgc
     2701 tttcactagc ccctgacccc tgacccctgg cccctgactc agttcgctgt cttcgcggcg
     2761 aagtcgacca gactcttgta gacatcggaa agacgggcgt cgaagctgcc gattttgcgt
     2821 ccattgatga acagcgttgg tgtgccggta acgtccactg acttgccgag ggcaatcgag
     2881 gcatcgacac gagctttcgt atccggctgg gccgcgcagg cggcgatgtc gcttcccttc
     2941 accccggctt gatccgcgat cgcggtcagt ttctcgtcgg cggtggcggc ggtaatgtcg
     3001 gcctgcgtct cataggtttt cgagatgaac ttccagaaag cgtcgttgga ggcacggccc
     3061 acgcaatcgg cgtaagcagc ccccttggcc gcccagttgt gcatctccag cggaaaattc
     3121 tgaaacacaa agtgcgcggt cggctcgacg gcaataagcg cctcgatcgt aggttgtgcg
     3181 gctttgcacg ctggacattg cagatcgcca aactcgacaa tcgtcaccgg agaattcttt
     3241 ggcccccggt ccgggcccgt gatgccttta tcgagagtct tcttgaccgg atcgaacggc
     3301 cgggcgccga acgggatgac gtcgcccacc agcgcatgct cgccgtccgg tgcgacgtaa
     3361 aaatggaata cctgctgccc ctcctgattg gccatgacag cggtcacctc cgccagcccg
     3421 gctattcctg ccggttttat gctcgcgagc ttccagctca ggccctcctg atagccaaat
     3481 tgctgctgca gaaaggactc gaccgtggct tccgagggaa gattgacatt ggccggagcc
     3541 ttgtctgcct tgggatctgg cttggccgcg ctcgattggg ccccggctgt ggccccgcta
     3601 agcacgagaa taagaatcgt cttcgtaaga ctgcgcatgg agtcctttcg tgtcaggaga
     3661 ttcagggttg cctcggggaa ggctgactta cagaatactt caaacttcaa tatttcagac
     3721 ctcgcatttg gcggcaatgg ggatgcgccg cccgtaacca aaagccttgg gagaaatctt
     3781 cagcccggga gccgcttgct ggcgcttgta ttcggcccgg tccaccatcc gaatcacgcg
     3841 ccgtacgact tcgatatcaa atccgtgatc ggcggcaatt cgctccgccg ggtgggaatc
     3901 ttcgacgtag tcctcgagta ccgcgtcgag gatgtcatac ggcggcagcg agtcgctgtc
     3961 tttctgattg gggcgcaatt ccgccgacgg cgccttctcc agcgtggccc ggggaatcac
     4021 aggacggcgg gagttgacgt agtggcttaa tcgataaacc agcgtcttgg gaacatcgga
     4081 gatgacggcc agtccgccca ccatgtcgcc atagagagtg cagtaaccga ctcccagttc
     4141 gctcttgttg cccgtcgaaa gcacgatcgc tccgaactta ttcgacagcg ccatcaacag
     4201 cgtgccccga gcccgcgact gaatgttttc ttccgtcaca tcttcttttt gcccggagaa
     4261 cacgccttgc agcgtctggt ggtaagcccc gtatgccgga ttgattgaga gaagctcaaa
     4321 gcgaatgccg agattcttgg ccagcgcgcg ggcatcatcg attgacccct gcgacgaata
     4381 cggacccggt atgtccacgc cgatgacatt ttccggcccc acagcatcgg cggcgatcac
     4441 ggcagtcagc gccgagtcga ttccgccgct tagcccgatg atggctttct gaaatccgca
     4501 cttgtgcatg tagtcgcgcg tgcccagcac cagggcggcg taggcgctgg cttcgtcgcc
     4561 ttcaatctgc tcgtgcatct ccccggttag agtctccgag tcaaaaaaca cgaggtcttc
     4621 ttcaaacgac cgcccctgcg cgacgacctt tccctctcga ttcagtacca ggctcgaccc
     4681 gtcgaatacc aggctgtcgt tgccgccgac ctgattcacc atgacgaccg gcaccttgtg
     4741 ctgggctgcg atgctggcca gcatttcacg ccggaaagcg cccttgccga tccagaaagg
     4801 cgaggccgaa atgttcagca cgaaattacc gcccgagtga atgagcgatt cgaccggatc
     4861 gaccgtgtaa agccgtttgc gccagaattg cttgtcgttc catgcgtctt cgcagatggt
     4921 gag
//
LOCUS       DASPUL010000576         3914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_107842, whole genome shotgun sequence.
ACCESSION   DASPUL010000576 DASPUL010000000
VERSION     DASPUL010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107842"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VEJ00_13400"
     CDS             <1..561
                     /locus_tag="VEJ00_13400"
                     /inference="COORDINATES: protein motif:HMM:NF014856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HXX02207.1"
                     /translation="DDQMRDWLVHHIAQPVVTGMKAEGAEFKGVLYCGLMMTARGPMV
                     LEFNCRFGDPETQPILMRLESDLVEALEASIEGRVSEGDFKWSQDAAVCVVMASGGYP
                     GTFEAGKRIDGLDEAGAAEGVKVFHAGTSKRDGAYYSSGGRVLGVTARAASLATAVAR
                     AYEACGKISFEGAHYRKDIAGRALKK"
     gene            703..1206
                     /gene="purE"
                     /locus_tag="VEJ00_13405"
     CDS             703..1206
                     /gene="purE"
                     /locus_tag="VEJ00_13405"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HXX02208.1"
                     /translation="MMSKPLVSIVMGSDSDLEIMREAGKALDEFGISYEIDVTSAHRS
                     PDRTAEYARKAAERGIRVIIAGAGGAAHLAGVIAAHTTLPVIGVPIPSTSLSGMDSLL
                     STVQMPAGIPVATVAIGKPGATNAGILAAQMIGIADEAIAKKLHGHKEKLARGVEEKS
                     RKLKSSM"
     gene            complement(1291..2166)
                     /gene="galU"
                     /locus_tag="VEJ00_13410"
     CDS             complement(1291..2166)
                     /gene="galU"
                     /locus_tag="VEJ00_13410"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HXX02209.1"
                     /translation="MMRLRKAVFPAAGLGTRFLPATKAQPKEMLPIVDKPIIQYGVEE
                     AMASGCDQIIIITGRGKQAIEDHFDVSYELEKMLEERRKTDLLRIVRNISDMIHVAYV
                     RQKEALGLGHAVLMARELVGNEPFAVLLADDVIDNPVPALKQMADVFQQKQCSVIAAQ
                     VVEGKMISSYGVFDAMPADGFNGRLFEIRNMVEKPRPEEAPSNLAIIGRYILTPSVFD
                     CLAKTQAGTGGELQLTDGMRMLLKQEKMYAYVYEGRRYDTGDKLGFLKATVEFALKRD
                     DLGGAFREYLKGLKL"
     gene            complement(2218..>3914)
                     /locus_tag="VEJ00_13415"
     CDS             complement(2218..>3914)
                     /locus_tag="VEJ00_13415"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HXX02210.1"
                     /translation="EFEDAFEFSETEDQAQAITDVMRDMESGQPMDRLLCGDVGYGKT
                     EVAMRAAFKAVGDNRQVAVLAPTTVLAFQHFETFKQRFGPFPVTIEMISRFRNPKQQK
                     EILQKTEAGKIDILIGTHRLLSKDIKFADLGLLIVDEEQRFGVRHKERIKQMRKQVDV
                     LTMSATPIPRTLHMSLVGLRDMSVIETPPKDRMAIQTIVASWDDKLIQSAIEQELDRG
                     GQVYFVHNRVESIWEIAAKLQELSPKARIIVGHGQMSEGELEKVMLKFMHHEADILVA
                     TTIIENGLDIPLCNTILINRADRLGLSELYQLRGRVGRSNRRAYAYLMIPREMELTPI
                     ARRRLAALKEFSDLGAGFKIAALDLELRGAGNLLGGEQSGHIEAVGFELYTQMLERAV
                     REMKGEAAPEEAETQLNLGLNIRIPADYIPEENQRLRMYKRVAGVETESQLADVGTEL
                     EDRYGPPPPAVRNLLDYASLKLLCIRVGVNVIERKRESVTLKFRQNAAVDPEQLARFV
                     SAQRGAQFTPDGMLKFVVKANAADEVLRTLRTVLEQLATIDVASLLDSSMSGNKSK"
BASE COUNT          722 a         1000 c         1356 g          836 t
ORIGIN      
        1 gacgatcaga tgcgcgattg gctggtgcat cacattgcgc agccggtggt cacagggatg
       61 aaggcggagg gcgcggagtt caaaggcgtg ctctactgcg gcctgatgat gacagcgcgc
      121 ggaccgatgg tcctggagtt caactgccgg tttggcgatc cggagacaca gccgattctg
      181 atgcggctgg aaagcgacct ggtcgaggcg ctcgaggctt cgatcgaagg gcgggtgagc
      241 gaaggggatt tcaagtggtc gcaggatgcg gcggtatgcg tagtgatggc ttcgggaggg
      301 tatccgggga cgtttgaggc ggggaagcgg attgacgggt tggacgaagc cggcgccgcg
      361 gagggcgtga aggtgtttca tgcaggaacg tcgaaacggg atggagcgta ttactcttcg
      421 ggcgggcgag tgctgggagt gacggcgcgg gctgcgtcgc tggcgacggc cgtggcgcgg
      481 gcgtatgagg cttgcgggaa gatttcgttt gagggcgcgc actatcggaa ggatattgcg
      541 gggagagcgt tgaagaagta agaaccccac ttctcgcgca aagagcgcgc gagaaatggg
      601 gcacccacgg ttgcggcaga tttcgtttac gacttcggtt acggagcttc gctccgccgg
      661 acagccgagg gcggctgtcc ctacaagaat tcttgagggt aaatgatgag caagccactg
      721 gtttcgattg tgatggggag tgattcggac ctcgagatca tgcgcgaggc ggggaaagcg
      781 ctggacgagt ttgggattag ctatgagatc gatgtaacct cggcgcaccg gtcgccggac
      841 cgaacggctg agtatgcgcg caaggcggcg gagcgcggga ttcgcgtgat cattgcagga
      901 gcgggcggag ccgcgcatct ggcgggagtg attgcggcac ataccacttt gcctgtcatc
      961 ggcgtaccga tcccttcgac gtcgctcagc gggatggatt cgctgctgtc gaccgtgcag
     1021 atgccggcgg gaattccggt ggcgaccgtc gcgatcggga agccaggggc gacgaacgcg
     1081 ggcattctcg cggcgcagat gattgggatt gccgatgagg cgatcgcgaa gaagctgcac
     1141 gggcacaagg agaagctggc gcggggtgtg gaggagaagt cgaggaagct gaagagttcc
     1201 atgtaagtca aaaagcccca ccgctcgcgc agaaggcgcg cgagaaatgg ggcacccaca
     1261 aatgaaatct tcagtctgag atcggggcga ttacagcttg agtcctttca ggtactccct
     1321 aaatgcgccg cccaggtcgt cgcgcttcag tgcgaattct actgtggctt tcaggaagcc
     1381 gagcttgtcg ccggtgtcgt agcggcggcc ttcgtagacg taggcataca ttttttcctg
     1441 cttgagcagc atgcgcatgc cgtcggtgag ctggagttcg ccgccggtgc cggcctgcgt
     1501 tttcgccaga caatcgaaga ctgagggcgt gagaatgtag cggccgatga tggccagatt
     1561 cgacggagct tcttccggtc ttggcttctc gaccatgttg cggatctcga acaggcggcc
     1621 gttgaaaccg tcggcgggca tggcgtcgaa gactccgtag gaagaaatca tcttgccttc
     1681 gaccacctgg gcggcgatca ccgagcattg tttttgctga aaaacatcgg ccatttgctt
     1741 gagcgcgggg acggggttgt cgatgacgtc gtcggcaagc aaaacggcga aaggctcgtt
     1801 gcccacgagt tcgcgcgcca tgagcacggc gtggcccagg ccgagcgctt ctttctggcg
     1861 gacgtaggcg acgtggatca tgtcggaaat gttacgaacg attcggagca gatcggtctt
     1921 gcggcgttct tcgagcattt tttcgagctc gtagctgacg tcgaaatgat cttcgatggc
     1981 ctgcttgccg cggccggtaa tgatgatgat ctggtcgcag ccggaggcca tggcctcttc
     2041 gacgccgtat tgaatgatgg gcttgtcgac gatgggcagc atctccttgg gctgggcttt
     2101 ggtggcgggc aggaagcgcg tgcccagtcc ggcggcaggg aagacggctt tgcggagtcg
     2161 catcatgaat gtttcgtttc tatttccgtg cacgattgta caagcaacat atttttccta
     2221 cttagacttg ttcccgctca tacttgagtc aagcaaagat gccacatcga tggtggcgag
     2281 ttgctccaga actgtgcgca gggtgcggag gacttcgtcg gcggcatttg ccttcaccac
     2341 gaacttcagc atgccatcgg gcgtgaactg ggcgccgcgc tgggcggaga cgaaacgggc
     2401 gagctgttcg ggatcgacgg cggcgttctg gcggaatttt aacgtcaccg actcgcgctt
     2461 gcgctcgatg acgttgacgc cgacgcgtat gcagagcagc ttaaggctgg cgtagtcaag
     2521 caggttgcgg acggcgggcg gcggagggcc gtaacggtct tcgagttcgg tgccgacgtc
     2581 ggcgagctgg gattcggttt cgacgccggc gactcgcttg tacatacgca ggcgctggtt
     2641 ttcttccgga atgtagtcgg ccgggatgcg gatgttgagg ccgaggttga gctgggtctc
     2701 agcctcttct ggcgcggctt cgcccttcat ctcgcggacg gcgcgctcga gcatctgcgt
     2761 gtaaagctcg aagccgacgg cttcgatgtg gccgctctgc tcgccaccga ggagatttcc
     2821 cgcgccccgc agttcgagat cgagagcagc gattttgaat cctgctccca aatctgaaaa
     2881 ttctttcaat gcagcaaggc ggcggcgtgc gatcggcgtc agctccattt cgcgcgggat
     2941 catcaagtag gcgtaggcgc gccggttgga gcggccgacg cggccgcgaa gctgataaag
     3001 ctcggacaat cccaggcggt cggcgcggtt gatgagaatc gtgttgcaga gcgggatgtc
     3061 gaggccgttc tcgatgatag tggtcgcgac aaggatgtcg gcttcgtggt gcatgaactt
     3121 cagcatcact ttttcgagtt cgccttcact catctggccg tggccgacga tgatgcgtgc
     3181 tttcggcgac agttcctgca gcttggcggc gatttcccag atgctctcga cccggttgtg
     3241 gacgaagtag acctggccgc cgcggtcgag ttcctgttcg atcgcggact gaatgagctt
     3301 gtcgtcccaa ctggcgacga tggtttgaat cgccatgcgg tctttgggtg gagtttcgat
     3361 gacgctcatg tcacgcaggc cgaccagcga catgtgcagc gttcgcggaa tgggcgtggc
     3421 cgacatggtc agcacatcaa cctgcttgcg catctgtttg atgcgctctt tgtggcggac
     3481 gccgaagcgc tgctcttcgt cgacgatgag caggccgaga tcggcgaatt tgatgtcctt
     3541 ggaaagcagg cggtgggtgc cgatcaggat gtcgattttg ccggcttcgg ttttctgcaa
     3601 aatctctttc tgctgcttgg gattgcggaa gcggctaatc atttcgatgg taacggggaa
     3661 cggtccgaag cgctgcttga aagtttcgaa gtgctggaag gcgagaacgg tggtcggggc
     3721 gagaacggcc acctgccggt tgtcgccgac agccttgaag gcggcgcgca tcgcgacttc
     3781 tgtcttaccg tagccgacgt caccgcagag caggcgatcc atgggctgac cggattccat
     3841 gtcgcgcatg acatcggtga tggcctgcgc ctggtcttcg gtctcgctga attcgaaggc
     3901 atcttcgaat tcgt
//
LOCUS       DASPUL010000577         3368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_943752, whole genome shotgun sequence.
ACCESSION   DASPUL010000577 DASPUL010000000
VERSION     DASPUL010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943752"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VEJ00_13420"
     CDS             <1..1139
                     /locus_tag="VEJ00_13420"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXX02211.1"
                     /translation="APPNASEAVRTRDDNPELAAAAKALFGYPYTDFSPQHAKWLLDK
                     KAELKKQLAGTAYFNSILDKLNTESSVANRAMMPDYLDPKRFPWVFFADSFMWPFNNE
                     RETARNPDEGVFIPLQEKMLHRWMQQENFSKLPASFSDYLKFITQVLEDNSKRGGIAM
                     KFEVAYFRPMTFSDPSRAQAEDLYQRYVGGGIPSERDYRTFQDFIFRYLLEEGGRLHL
                     PVHIHTAIGIGDYFNLSQSNIMSLESVLRDPRYASTTFVMIHGGYPLEREAIWLAAMK
                     NVYLDSSFGELAQYPSAFKDTLKMWLETFPDKITFGTDCFPYNQVLGAEESYWLGVQS
                     SRTALAAALAEMVSEGEITEARALELAHAYLHDTAVRLYGGRVH"
     gene            1238..1621
                     /locus_tag="VEJ00_13425"
     CDS             1238..1621
                     /locus_tag="VEJ00_13425"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXX02212.1"
                     /translation="MTKHAAGPRELQYIPWTSVPLEDLNPLFQRQFVVGQEIMLARVL
                     LKKGCVVPEHSHHNEQLTYILEGALKFWIDGREIVVNAGEVLCIPSNMPHKAEALEDT
                     IDLDVFCPPRADWINKTDQYLRGRK"
     gene            1631..2992
                     /gene="thrC"
                     /locus_tag="VEJ00_13430"
     CDS             1631..2992
                     /gene="thrC"
                     /locus_tag="VEJ00_13430"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXX02213.1"
                     /translation="MSPFPTSAPAGQMDFLFRCIGCEDLSSDAGQDFRCSRCGDLLEI
                     TYPDWKQAKPDTEHLRAAWHNRRLSPKAIDLSGVWRFRDVLPRLDDSEQVITLREGNT
                     PLYDLPRCARIAGVPRLFAKHLGMNPTGSFKDGGMTVAATFARRAGFRWVACASTGNT
                     SASMAAYAARGGLRSLVLVPEGKISWSKLSQSLDYGAVTCQLRTDFDGCLRLLQELVL
                     RAPVYQLNSINPFRLEGQKTVALELLEQLDWQAPHHIIVPGGNLGNSSAIGKALLEMR
                     DLGLIAKLPKLSVIQAEGANALVRTLREAAGKHLVSVQAETRATAIRIGNPASWKKAV
                     RVLEATGGACEQVSEMEIAQAKAEIGAEGIGCEPASAVTLAGLKKLVKQKFVKPEETV
                     VLILTGHLLKDPDFTIEFHRGELFKGSAGNVSALNPLRRPPAVLDATIDAVMRTLEQA
                     EKS"
     gene            2999..>3368
                     /locus_tag="VEJ00_13435"
     CDS             2999..>3368
                     /locus_tag="VEJ00_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02214.1"
                     /translation="MPSRSGPVKSALRLALPATSANLGPAFDAAALAMDFHIQVDARA
                     SEDFSITASGRDREICAQLDNHLILTTYRDVLQFAGAPVIPLSLRIANEIPIGKGCGS
                     SAAARLAGIALAVHFGRLRWS"
BASE COUNT          717 a         1005 c          975 g          671 t
ORIGIN      
        1 cggcgccgcc caatgccagc gaagctgtcc gcacgcggga tgacaatccc gagctcgctg
       61 ctgcagccaa ggctctgttt ggttatccct acaccgactt ttctccccaa cacgccaagt
      121 ggctgctcga caagaaagcg gaactgaaaa agcagctggc ggggaccgcc tacttcaact
      181 cgattctcga caagctcaac acggagagct ccgtggcgaa ccgcgcgatg atgcctgact
      241 acctcgatcc gaagcggttt ccctgggtct ttttcgccga ctcgttcatg tggcctttca
      301 acaatgagcg ggaaaccgcg cgcaacccgg acgaaggtgt gtttatcccg ctgcaggaaa
      361 aaatgctgca ccgctggatg caacaagaga atttcagcaa gctgccggcc agcttttccg
      421 actatctgaa attcatcacc caggttcttg aggacaactc gaagcgcggt ggcatcgcca
      481 tgaagtttga agtcgcctat tttcggccga tgacgttcag cgacccctcc cgagcgcaag
      541 cggaggatct ttaccagcgt tacgtcggcg gcggaatccc cagcgagagg gattaccgca
      601 cgtttcagga cttcatcttc cgctatctcc ttgaagaggg tggccgttta caccttccgg
      661 tgcacatcca caccgcgatc ggcatcggcg attatttcaa tctgtcacaa agcaacatca
      721 tgagcctgga gagcgtgctg cgcgatccgc gctatgcctc gacgacgttt gtcatgattc
      781 acggcggcta ccctctggag cgcgaagcca tctggctggc cgccatgaaa aatgtttatc
      841 tcgattcttc ttttggggag ttggcgcagt atccttcggc cttcaaagac acgctgaaga
      901 tgtggctcga aacttttccc gacaagatca cattcggtac ggactgtttc ccttacaatc
      961 aggtattggg cgcggaggag agctactggc tgggagtgca atcgtcacga acagcgctgg
     1021 ccgcagcgtt ggcagagatg gtgtccgaag gagagattac cgaagcccgg gcgcttgagt
     1081 tagctcacgc ctatttgcat gacacggcag taaggctcta tggaggcaga gtgcactgaa
     1141 agccatggcc aaaaagaaat tgaaagccaa gtcaagagcg agaacgatcc gagcaacagc
     1201 aaccagggcg gcttcacaca gtcgcgcgcg gcgagccatg acgaaacatg cggccggccc
     1261 cagggagctc caatacattc cgtggaccag cgtgccgctg gaggatctca atcccctgtt
     1321 ccagaggcaa ttcgtagtcg gacaggaaat catgctggca cgggttctgc tgaagaaagg
     1381 atgtgtcgtc cccgaacata gtcaccacaa tgagcaactc acctacatcc tcgaaggcgc
     1441 gctcaagttc tggatcgacg gcagggagat cgtggtaaat gcgggcgaag tgttgtgcat
     1501 cccgtccaat atgccgcaca aggcggaggc gctggaagat acgattgacc tcgacgtctt
     1561 ctgtccgccg cgcgccgatt ggatcaacaa gactgaccaa tatcttcggg gaagaaaata
     1621 actccgggcg atgagtcctt ttcccacgtc tgcgcccgcc gggcaaatgg attttctctt
     1681 tcggtgcatc ggctgcgagg atctcagcag cgacgccggg caggacttcc gctgttcccg
     1741 ctgcggagat ctgctcgaaa tcacataccc cgattggaag caggcaaagc ccgacacaga
     1801 gcatctgagg gctgcgtggc acaatcgccg actctcgccg aaagcgatcg acctcagcgg
     1861 cgtgtggcgt tttcgcgatg ttcttccccg gctcgatgac tcagaacagg tcatcacgct
     1921 ccgggagggc aacacgccgc tttacgattt gcctcgctgc gcccgaatcg ccggtgtgcc
     1981 gcgtctattc gccaaacacc tgggaatgaa tcccactggg tccttcaagg acggtgggat
     2041 gactgtggcg gcgaccttcg cccggcgcgc cggctttcgc tgggtggcgt gtgcttcgac
     2101 gggcaacacg tctgcatcga tggctgccta tgccgcgcgc ggaggcctgc gcagcctggt
     2161 tctcgtcccg gaggggaaaa tctcatggag caagctgtcc caatctcttg actacggcgc
     2221 cgtcacctgc cagcttcgca ccgacttcga cggctgtctg cggcttttgc aagagctcgt
     2281 cttgcgcgct ccagtctatc aactaaattc gatcaacccg ttccgtctgg aaggccagaa
     2341 aacggttgcg ctggagttgc tcgaacaact tgactggcaa gccccccatc acatcatcgt
     2401 tccgggcggc aatctcggca acagttcggc gatcggaaag gcgctgctcg aaatgcgcga
     2461 cctcggtctc atcgcgaagc tgccgaagct ctcggtgatt caagccgaag gggcgaatgc
     2521 actcgtgcga accttacgcg aagccgcggg caagcatctc gtgagcgtgc aagcggaaac
     2581 tcgggctacc gccattcgga tcggcaatcc cgcttcgtgg aagaaagccg tgcgcgttct
     2641 cgaggccact gggggcgctt gcgagcaggt cagcgaaatg gagattgctc aggccaaggc
     2701 cgagattggc gcggaaggaa tcggctgtga gccggcatcg gcggtcactc tggctggcct
     2761 gaagaaactc gtgaagcaga aattcgtgaa gcctgaagag accgtggttc tcattctgac
     2821 cgggcatctg ctgaaagatc cagacttcac gatcgagttt caccgcggcg aactgttcaa
     2881 gggcagcgcg ggcaacgtga gcgcgctcaa cccactgcgc cggccgccag cagtactgga
     2941 tgccactatc gatgcagtaa tgcgcacgct ggagcaagcg gagaaatcct gaggaatcat
     3001 gccatccaga tccggtcccg tgaaatcggc tcttcgcctg gcgcttcctg ccacttcagc
     3061 caacctcggc cccgctttcg atgccgccgc gctggcaatg gatttccaca tccaagtcga
     3121 tgcgcgtgcg tctgaagatt tctcgattac cgccagcgga cgagatcgcg aaatctgcgc
     3181 acagctcgac aaccacctca ttctgaccac ctaccgtgac gtgctccagt tcgcgggagc
     3241 acctgtcatt ccgctctcgc ttcggatcgc gaatgagatt ccaattggca agggctgcgg
     3301 ctcttcggcg gcggcgcgct tggcaggcat cgcgctggcc gtacacttcg ggcgcctgcg
     3361 ctggagtg
//
LOCUS       DASPUL010000578         4139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2031765, whole genome shotgun sequence.
ACCESSION   DASPUL010000578 DASPUL010000000
VERSION     DASPUL010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2031765"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VEJ00_13440"
     CDS             complement(<1..217)
                     /locus_tag="VEJ00_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02215.1"
                     /translation="MQSTFDERNWLAWLVRVRIFILTLLLAVELAVAEFSPAPVPMRL
                     FLSTILFWFSLSLFYVLLLSFWQEHRVQ"
     gene            complement(382..1590)
                     /locus_tag="VEJ00_13445"
     CDS             complement(382..1590)
                     /locus_tag="VEJ00_13445"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXX02216.1"
                     /translation="MPVFTFSGKDASGQKISGERIAPNKQALAQALRRDRITPGAIRE
                     KGKEFSLPTFGSGKVTTKEIAVFFRQFSVMIDAGLPLVQCLEILAANQENQAFQKTLT
                     GVRTTVEGGATLANAMRQYPAAFDELTTNMIEAGETGGILDIILQRLATYVEKAVRLR
                     SAVKSALIYPVAVVSIAFLIVGALLKWVVPIFANLFAGLGVNLPLPTRITMGLSAFVQ
                     TFWWLFLIGIVGMFFGVKQIRKHPRGKYYFDKGLLHLPVIGSLLRKIAVGRFTRTLGT
                     LITSGVPILEGLTITAKTSGNAVLEEALMKVRKAIEEGRTIVDPLRECGVFPNMVVQM
                     IGVGEATGAMDSMLQKIADFYEEEVDAATKDMLAMLEPIIIGLLGVMIGGIVISLYMP
                     LFAMIAKLAG"
     gene            complement(1663..2799)
                     /locus_tag="VEJ00_13450"
     CDS             complement(1663..2799)
                     /locus_tag="VEJ00_13450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXX02217.1"
                     /translation="MGLSLSDLLKRMLEMNGSDLHITTNSPPQIRVHGHLVPLDLPQM
                     TPAETKQLAYSVMTDSQKHRFEESLELDFSFGLKGLARFRANVFNQRGATAAVFRLIP
                     FEIKSFNQLGLAPVVSKLCDKPRGLVLVTGPTGSGKSTTLAAMIDKINSERHDHILTI
                     EDPIEFVHMNKNCVVNQRELHADTKSFSDALRAALREDPDVVLIGEMRDLETIESALR
                     IAETGHLTFGTLHTNSATSTINRIIDVFPAHQQPQIRAQLSLVLEGIMCQSLLPRTDG
                     KGRAMIMEILVPNAAVRNLIREDKIHQIYSAMQSGQEKFGMQTFNQALATAYFQKQIS
                     MEVAMARSSNPDELTEMINRGASLLSRPTTAGTTLARGAAPSKR"
     gene            complement(2838..>4139)
                     /locus_tag="VEJ00_13455"
     CDS             complement(2838..>4139)
                     /locus_tag="VEJ00_13455"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXX02218.1"
                     /translation="YGATKEEDLETVMQSMSELSDEDVELQGEQQEMELSELEKAADE
                     APVVKLVNVILGDAVKRGASDIHIEPYEKEFRVRFRIDGVLQTIMSPPMKLRDAMTSR
                     LKIMSKLDISEKRLPQDGRIMLKMNIGGRKKQLDFRVSTLPVLWGEKIVLRLLDKENL
                     RLDMTKLGFEPESLVKFEKAILKPYGMVLVTGPTGSGKTNTLYSSISRLNQPDTNIMT
                     AEDPVEFQLQGVNQVQMKEQIGLNFAAALRSFLRQDPNIILVGEIRDFETAEIAIKAA
                     LTGHLVLSTLHTNGAPETITRLMNMGIEPFLVATSVHLICAQRLVRRICKDCTEVVEV
                     PVQTLIDAGFSPEEAKTVKIQKGKGCGVCNNTGYKGRCGLYEVMEVDDEIRELVLVGA
                     SAVELKKKAIERGMLTLRRSGLIKVAAGMTTLEEVARETIH"
BASE COUNT          772 a         1237 c         1269 g          861 t
ORIGIN      
        1 cctgaacgcg gtgctcctgc cagaaggaga gcagcagcac gtaaaacagc gagaggctga
       61 accagaacag gatcgtgctc aggaacaacc gcatggggac gggcgccggg ctgaactcgg
      121 cgaccgcgag ttcaacggcc agcagcaggg tcaggatgaa aatccgcacc cggaccaacc
      181 aggccagcca attgcgctcg tcaaacgtgg attgcattac gatcctgtgg ccctggcgtc
      241 ggtcagattt ctcgagcttc ttgcgtcaac tccgggccca agaccgagca cgtcagactt
      301 aagaccctaa gatcggcgac cgacacgcgc ttagccggag gccggagctg tcgcgatccg
      361 gcctcccggc gttgttctgc attagcccgc cagcttggcg atcatggcga acagcggcat
      421 gtagagcgaa atcacgatgc cgccgatcat gacgcccaac agaccgatga tgatcggttc
      481 cagcatggcc aacatgtcct tggtggccgc gtccacttct tcttcgtaga agtcggcaat
      541 cttctgcagc atggagtcca tcgcaccggt cgcttcgccg acgccgatca tctgcaccac
      601 catgttggga aacacgccgc actcccgcag cggatcgacg atggtgcggc cttcttcgat
      661 cgccttgcgc accttcatca gggcttcttc cagcaccgcg ttgcccgaag tcttggccgt
      721 gatcgtcaag ccttcgagaa tcggcacgcc tgaggtgatc agggtaccca aagtacgggt
      781 gaagcggccg accgcgatct tgcgcagcag cgagccgatg accggcaggt gcaacaagcc
      841 tttatcgaag tagtacttgc cgcgcggatg cttgcggatt tgcttcacgc cgaagaacat
      901 gccgacgatc ccgatcagga agagccacca gaaggtctgc acgaaggcgc tcaggcccat
      961 ggtgatgcga gtggggagcg gcaggttgac gcccagcccg gcgaacaggt tggcgaagat
     1021 cggcaccacc cacttgagca gcgcgcccac gatcaggaat gcgatcgaaa cgacggcaac
     1081 gggatagatc agggctgact tcaccgcaga acgcaggcgg acagcctttt ccacgtaggt
     1141 cgccaggcgc tgcaggatga tgtcgagaat accgccggtt tcgcccgcct cgatcatgtt
     1201 ggtcgtgagt tcgtcaaagg cggcggggta ctggcgcatg gcgttggcca gcgtggcgcc
     1261 tccttccacc gtggtgcgca cgccggtgag ggttttctgg aaagcctggt tctcctgatt
     1321 ggcggccaga atttccagac actgcaccag cggcaggccg gcatcgatca tgaccgagaa
     1381 ctggcggaag aagaccgcaa tctccttggt cgtgaccttg cccgagccga acgtgggcag
     1441 agagaattct ttgcctttct cgcgaatcgc gccgggggtg atgcggtcgc ggcgcaacgc
     1501 ctgcgccagc gcctgtttgt tgggcgcgat gcgctctccg gaaatcttct gtccggaggc
     1561 gtccttgccg gaaaatgtaa agactggcat ggtgttagcc tctcctcaat gacgtgcctg
     1621 cttgctgaaa actctttcct gcttcatttc tccccgcccc tcctaacgct tggagggagc
     1681 ggccccgcgg gccagagtcg tcccggctgt cgtgggacgg cttagcaagc tggcgccgcg
     1741 gttgatcatt tccgtcaact cgtcgggatt gctggaccgc gccatggcca cttccatcga
     1801 aatctgcttc tggaagtaag cggtggccag ggcctggttg aacgtttgca tgccgaactt
     1861 ctcctggccg gactgcatgg ccgagtagat ctggtggatc ttgtcttcgc ggatcagatt
     1921 gcgcacggcg gcgttgggca ccaggatctc catgatcatg gcacggccct tgccgtcggt
     1981 gcgcggcagc agcgattgac acatgatgcc ttccagcacc agcgaaagct gggcacgaat
     2041 ctgcggctgc tggtgggcgg ggaagacgtc gatgatacgg ttaatcgtcg acgtggccga
     2101 gttcgtgtgc agggtgccga aggtcaggtg gccggtttcg gcgatgcgca gcgccgattc
     2161 gatggtttcc aggtcgcgca tttcgccgat gagcacaacg tcgggatctt cacgaagcgc
     2221 ggcgcgcagc gcgtcggaga agctcttggt gtcggcgtgc aactcgcgct ggttgaccac
     2281 gcagttcttg ttcatgtgca cgaattcgat cgggtcctcg atggtgagga tgtggtcgtg
     2341 gcgctcgctg ttgatcttgt cgatcatggc cgccagcgtg gtggactttc cggagccggt
     2401 ggggccggta accagcacca ggccgcgcgg tttgtcgcac agcttgctca ccacgggcgc
     2461 caggccgagc tgattgaaag acttgatctc gaaaggaatc agtcggaaga cagccgccgt
     2521 ggcgccgcgc tggttgaaga cgttggcgcg gaagcgagct agtcccttca agccgaagga
     2581 gaagtccagt tccaggctct cttcaaagcg atgcttctgg gaatcggtca tgacgctgta
     2641 ggccagctgc ttggtctcgg cgggcgtcat ctgcggcaga tcgagcggca ccaggtgccc
     2701 gtgcacacga atctgcggcg gcgaattcgt ggtgatgtgc aggtcgctgc cgttcatctc
     2761 cagcattctt ttcaacagat cgctaagcga caaacccata agtagttcct tcctctaact
     2821 ggctcgtgca attacgacta gtgaatcgtt tcgcgggcga cttcctcgag cgtggtcatg
     2881 cccgccgcaa ccttgatcag cccgcttcgg cgcagtgtca gcatgccgcg ctcgatggct
     2941 ttcttcttca actccacggc cgacgcgccg accagaacca gctcgcgaat ctcgtcgtcg
     3001 acctccatga cctcgtacag accgcagcga cccttgtagc cggtgttgtt gcacacaccg
     3061 cagcctttgc ccttctgaat cttgaccgtc ttggcctctt cggggctaaa gccggcgtcg
     3121 atcagcgtct ggaccgggac ctcgaccacc tcggtgcagt ctttgcaaat ccggcggacc
     3181 aggcgctggg cgcagatcag gtggaccgac gtagccacca ggaacggctc aatacccatg
     3241 ttcatcaagc gggtaatggt ttccggggcg ccgttggtgt gcagtgtcga gagcacgagg
     3301 tggccggtaa gagccgcttt gatggcaatt tcggcggtct cgaagtcgcg gatctcgccg
     3361 accagaatga tgttggggtc ctgccgcagg aacgaacgca gagccgcggc gaagttcaag
     3421 ccgatctgtt ccttcatctg cacctggttg acgccttgca actggaactc gacggggtcc
     3481 tcggccgtca tgatgttggt gtcgggctgg ttcaggcggg aaatcgacga gtacagcgta
     3541 ttggtcttgc ccgatcccgt tgggcccgtg accagcacca tgccgtaggg cttgagaatg
     3601 gctttttcga acttgaccag cgattccggc tcaaaaccaa gcttggtcat gtcgaggcgc
     3661 aggttttctt tgtcgagcag ccggagcacg atcttttcgc cccacaacac cggcagcgtg
     3721 ctgacacgaa agtcgagctg cttcttgcgg ccgccaatgt tcatcttcag catgatgcgg
     3781 ccgtcctgcg gcagccgctt ttcgctgata tcgagcttcg acatgatctt cagacgcgac
     3841 gtcatggcgt cccttaactt catcggcggc gacatgatgg tctgcagtac gccgtcaata
     3901 cggaaccgga cgcggaattc cttctcgtag ggctcgatgt gaatgtcgct ggcgccgcgc
     3961 ttcacggcat cgcccaggat gacgttgacc agcttcacga cgggagcttc gtcggcagcc
     4021 ttttccagct ccgacagctc catctcctgt tgttctccct gcaattcgac gtcttcgtcg
     4081 ctcagctccg acatcgactg cattacggtt tcgagatctt cttccttggt cgcgccgta
//
LOCUS       DASPUL010000579         4037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1335033, whole genome shotgun sequence.
ACCESSION   DASPUL010000579 DASPUL010000000
VERSION     DASPUL010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335033"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..81
                     /locus_tag="VEJ00_13460"
     tRNA            7..81
                     /locus_tag="VEJ00_13460"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Cys,seq:gca)
     gene            295..2517
                     /locus_tag="VEJ00_13465"
     CDS             295..2517
                     /locus_tag="VEJ00_13465"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02219.1"
                     /translation="MNSRPFLFLASVLILVLVRIMTTAAKRRGHPFFQSEIESEVWSS
                     RRLLIASFLTLFAELAFIRWVAVEIRVFAYFKNLALLLCFVGFGLGCALGRQAIRWPV
                     CLKAIMGLLVVVRMPWRSGKALEGISQTLGGGADLDLWANPRVWNWVDFLSVAVLTAI
                     LFLLLVWIFIPMGQIVGRQMNLAPRPLIGYSWNLFGSLVGILAFLGVSRMMLPPAVWL
                     GIVLAGFALLQARSRERALVLILLVPLVMLLHDPATRDHYEIWTPYQQITYSRFYAQN
                     GDFAEGEVKVNHTGYQAIVNLSADFLARHPKLLKEAEGENPYNVAYRFVRPSPSVLIV
                     GSGTGNDVAAAIRHNSSVVDAVEIDPAILALGKREHPERPYDSPAVSAHLTDARAFLK
                     RTARRYDLVMFGLLDSHTQFSDYSNMRIDNFVYTMESFHEAVEHLDPDGVLFVKFAVE
                     RPWMGRRLAEMLQQTFGKPPVVFFAASSYTPYAVCFAISRSGRVEDALRGDPSLAQFV
                     SQHPFATDAPDVPVTTDDWPYLYQQGRSIPRTYLTLGVLVVLVTVGLYLKIPETRREA
                     PSLFYFAMGAGFMLLETQAISRLALYFGTTWQVNGIVISVLLTALLAANLAVEHFPKS
                     MPKTWIAVPLLAGLLAVYWLPFSRMQGTPAVVGALVAGVFAVPVIFAGLLFSLEFRDA
                     PSPAAALGANVLGAVVGGLLENLSLIVGMRGLIPITIGMYCIAALALSLRHRATFHSA
                     "
     gene            2630..>4037
                     /locus_tag="VEJ00_13470"
     CDS             2630..>4037
                     /locus_tag="VEJ00_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02220.1"
                     /translation="MIWYFVFFFLSGFCSVLYELVWLRLSMAQFGVTTAMVSIVLSVF
                     MAGLGLGSWASGRWLSGPDSTPALSALRLYALTELLIGLSGILVRYELLWGHTILEHA
                     GMSSSVGYYVLAGFWVAFTLLPWCTLMGATLPIGMRAIAETVPRESRRSFSYLYTANV
                     LGAVVGTTVPLFLIELLGFRGTLKVGAAGNCLIAVSAFALSNFAKSSVVPSQPPAETP
                     QPIAMPTPSVGGNRILGLLFLSGLTSMAMEVVWVRSYTPYCGTVVYAFASILGVYLLS
                     TYAGSVVYRRWSIRHLRENPVVWSLLASFALLPLIAASPVVHLARIPRLVLGITPFTG
                     LLGFITPMLVDRFSAGNPGRAGTAYATNVVGCILGPLLAGFGLLPFISEPLALIVLAL
                     PWLIAGLAPLRPALKPSRPLRVAIAGSVALAAVLIAAGQGYEERYRGGRILRDSTATV
                     IATGTDMDKALLVNGYGMT"
BASE COUNT          633 a         1201 c         1219 g          984 t
ORIGIN      
        1 gtcaggggcg cggtacccaa gtggtaaggg agaggtctgc aaaaccttta tgcgtcggtt
       61 cgattccgac ccgcgcctcc aaattcttga cccggctgcc tcggtgcttt ccctgggtcc
      121 ttcgctccgc tcaggatttc gccagcggtc tctcgcttcg ctcacgcccg ctaatcggct
      181 caagttcgat tccgacccgc gcctccaaac tttaccggcg aattccagtc gagcagcggg
      241 ccttccgcat gatttgctcg cgtcttgcat tcccctgaac tcctccggct caagatgaac
      301 tctcgaccat tcctgttcct cgcctcggtc ctgatcctgg tactggtgcg gatcatgacc
      361 acggccgcga agcggcgtgg gcaccccttc tttcaatccg agatcgaatc cgaggtctgg
      421 agtagccgtc ggctactcat tgccagtttc ctgaccctgt tcgcggagct ggccttcatt
      481 cgctgggttg cagtcgagat cagggttttt gcctatttca agaatctcgc cttgctcctc
      541 tgcttcgtgg gttttggcct gggatgcgcc ctgggacgac aggccattcg ctggccggta
      601 tgcctgaagg ccatcatggg cctgctcgtg gtggtgcgca tgccgtggcg gagcgggaag
      661 gccctggagg gtatttcaca aaccctcggc ggcggcgctg atcttgatct atgggccaac
      721 ccaagggtct ggaattgggt ggattttctt tccgtcgccg tgttgactgc cattctgttt
      781 ctgttgctgg tctggatctt catcccgatg gggcagatcg tcggcaggca gatgaatctg
      841 gcgccccggc cgctgatcgg ctactcgtgg aatttgtttg gcagcctggt cggcattctg
      901 gcgtttctcg gagtgagccg gatgatgctc ccgcccgcgg tctggctggg aattgtgctg
      961 gccggattcg ccttgctcca ggccaggagc agggagcgcg ctctcgtcct cattctgcta
     1021 gttccgctgg tgatgctgct tcacgatcct gccacacgcg atcactatga aatctggacg
     1081 ccatatcagc agatcaccta ttcccgcttc tatgcgcaga atggcgactt cgccgagggc
     1141 gaagtgaagg tcaaccacac gggctatcag gcgatcgtta atttgtcggc ggactttttg
     1201 gcgcgacacc caaagctgct taaggaggca gagggagaaa atccctacaa cgtcgcttac
     1261 cggtttgtgc ggccttcgcc gagcgttctg atagtcggct ccggaacggg caacgatgtc
     1321 gcggcagcaa tacgccacaa cagctctgtg gtggatgcgg tcgagatcga tcccgccatt
     1381 ctggccctgg gaaaacggga gcatcccgag cgcccttatg attcgccggc ggtttccgct
     1441 catctgaccg atgcacgggc gtttttgaag cggactgcgc ggcgctatga cctggtgatg
     1501 tttgggctgc tcgactcgca tacccagttc tcggattatt cgaacatgcg gatcgacaac
     1561 ttcgtctaca ccatggaatc gttccatgaa gcggtcgagc acctggatcc ggacggcgtg
     1621 ctgttcgtga agttcgctgt ggagcggccc tggatggggc gccgcctggc ggagatgttg
     1681 cagcagacct tcggcaagcc tcctgtcgtg ttctttgctg cctccagcta cacgccctac
     1741 gcggtgtgct tcgccatttc ccgctccggc cgggttgaag acgcccttcg cggcgacccc
     1801 agcctcgcgc agttcgtgag ccaacatccc tttgcgaccg atgcacccga cgtgccggtc
     1861 acgaccgacg actggccgta tctctatcag cagggccgca gcattccccg cacgtatttg
     1921 acgctcggcg tgctggtcgt tctggtcacg gtagggttgt atctgaaaat tccagagact
     1981 cgccgggagg ctccatcgct gttctacttc gccatgggcg ccggcttcat gctgttggag
     2041 acccaggcga tcagtcgtct ggcactgtat ttcggcacca cgtggcaggt gaacggaatc
     2101 gtgatttcgg ttctgttgac ggcgttgctg gctgcaaacc tcgcagtcga gcacttcccg
     2161 aaatcaatgc ccaagacgtg gatcgccgtg ccgctgcttg caggtctgct ggctgtgtac
     2221 tggctcccgt tcagtcgaat gcagggcacc cccgcagtgg tgggagcatt ggtcgccggc
     2281 gtgtttgctg tgcccgtaat tttcgctgga ttgctgttct ctctggaatt ccgcgacgcg
     2341 ccatcgcccg ccgcggctct gggtgcgaat gtcctgggtg ccgttgttgg aggcctgctg
     2401 gaaaaccttt cactcatcgt cgggatgcgg ggattgattc cgattacgat cggaatgtat
     2461 tgcatagccg cgcttgcact ttctctccgg catcgcgcga cgtttcactc cgcgtgaatc
     2521 cccgcgactc gggcatggcg agctggcttg cctgtcccca ccaatctctc tacactcgtt
     2581 gccgagcctc atcacatcct gcgcatagac caacgggggt agaccgctct tgatctggta
     2641 cttcgttttc ttttttctct caggcttctg cagcgttctc tacgaactgg tgtggctgcg
     2701 cttatccatg gcgcaatttg gagtgaccac cgccatggtc tcgatcgtgc tctcggtgtt
     2761 catggcgggg ttgggcctcg gatcatgggc gagtggacgc tggctcagcg ggccggacag
     2821 cacgcccgca ctatcggcgt tgcgtctcta tgcgctgacg gagttgctga tcggactgtc
     2881 agggattctt gtacgttacg agctgctgtg gggtcacacg atccttgaac acgcgggaat
     2941 gtcctcttcc gttggttatt acgttctcgc aggtttctgg gtcgcattca cgttgctgcc
     3001 ctggtgcacg ttgatgggtg ccacgctgcc gatcggaatg cgagccatcg cggaaacggt
     3061 tccgcgcgaa tcgcgccgtt catttagcta tctctatacg gcgaacgttc tgggcgcagt
     3121 tgtggggacg acggtgccgc tgttcctgat cgaactgctt ggctttcgcg ggacgctgaa
     3181 agttggagca gcgggcaact gccttatagc ggtatccgcg tttgcgctgt cgaactttgc
     3241 gaaatcgagc gttgttccga gccaacctcc tgctgaaact cctcagccga ttgctatgcc
     3301 gacgcccagt gtcggcggga acaggattct cggtctgctt ttcctgagcg gcctgacctc
     3361 gatggcgatg gaggtcgttt gggtgcgcag ctatacgccc tattgcggaa ccgtcgtcta
     3421 tgcgttcgcc tcgatcctgg gcgtctatct cttgtctacc tatgccggct ctgtggtgta
     3481 tcgcagatgg agtatccggc acctgcggga aaatcccgtc gtatggagcc tgttagcgtc
     3541 gttcgccctg cttccgctga ttgcggcaag ccctgtagtc catctggcgc gaataccccg
     3601 gctggtgctc gggatcacgc catttaccgg cttgctgggt ttcataacac ccatgctggt
     3661 ggatcgtttc tcggcgggaa accccggcag agcaggcacg gcttatgcga ccaacgttgt
     3721 gggttgcatc ctgggacctc tgctggccgg tttcggattg ctgccgttca tcagcgagcc
     3781 tttggctctg attgtgctcg cactgccctg gctgatcgcg gggctcgcgc cgctgcggcc
     3841 ggcgttgaaa ccgagtcgtc cgttgcgcgt ggccatcgcg ggatcggtcg cactggccgc
     3901 ggtgctcatc gctgccggac aaggctacga ggagcgctat cgcggcggca ggatacttcg
     3961 agattccacg gcgacggtaa tcgccaccgg cacagacatg gacaaggcac tactggtcaa
     4021 cggctatggc atgacgt
//
LOCUS       DASPUL010000580         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1884417, whole genome shotgun sequence.
ACCESSION   DASPUL010000580 DASPUL010000000
VERSION     DASPUL010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1884417"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VEJ00_13475"
     CDS             complement(<1..812)
                     /locus_tag="VEJ00_13475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX02221.1"
                     /translation="MPRNRYMVVIVFLTFFVMSALTNILNALVPDIISSFHVGLAAAA
                     FLPFSFFIAYGVMSIPAGFLVERFTEKPVMIAAFLAGTAGSLSLAAHPSYRVAVASLF
                     VMGSGMATLQTAINPLLRVAGGEEHFAFNSALAQLIFGSASFLSPYVYSYLVVNLADF
                     SAHRNLLLTVLRRLTPPALPWASMYWIFAVFTLLMIVVLYFSRFPQVEHTAEEQAGSW
                     EMYGSLFRKPMVWAFFFCVFAYVGSEQGTADWISKFLSQYHGYDPHTTGAAAV"
     gene            complement(831..1880)
                     /locus_tag="VEJ00_13480"
     CDS             complement(831..1880)
                     /locus_tag="VEJ00_13480"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HXX02222.1"
                     /translation="MLSEFSVIDGEYLRDLLDQPRALSQTLEGLEVTKPLRELAARLQ
                     KGKFKTVVLTGMGSSFHALHPLNIELINHGLTAMMVETSELLHYRSRLFHHQTLIVAV
                     SQSGQSAEVVRLLEINRGRSSIIAITNTPDSPLAERADATILTRAGKEFSVSCKTYLS
                     ALMALQWLGDVVSGRELRRTRQELKAAGPAVDEYLKHWKEHVQQLGQLLEGIRHLFLV
                     GRGASLAAVGTGALIVKEADHFHAEGMSSAAFRHGPLEMLSDETFVLVFAGENKTRDL
                     NQRLFDDVREQQGRTEMISEDSASSACVLPAAPRSIHPILEILPVQMVTLALAAQVGR
                     EPGRFELASKVTTRE"
     gene            complement(1870..>2539)
                     /locus_tag="VEJ00_13485"
     CDS             complement(1870..>2539)
                     /locus_tag="VEJ00_13485"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXX02223.1"
                     /translation="RDLEQIFHVSVAMENDADAAAVGEAGWGAGRGRKSLIYVTVGTG
                     IGVGIILEGRVYRGVDRAHPEIGHHLIDPSGPLCDCGFHGCWESLAAGPSMVKWIERN
                     APLDYHHRIGLTAKRICELAQEGDAWAVRAIEHESRYLGLGLANLVVMFVPEIIVLGG
                     SVMQSASLFMEGIRKVIASSCRFVPYEKTELALASLGSDTNLIGAGRVWYYRCGSEAE
                     KNAV"
BASE COUNT          587 a          759 c          721 g          472 t
ORIGIN      
        1 acggcagcag cacccgtcgt atgtggatcg tagccgtgat attgcgatag gaacttcgaa
       61 atccagtcgg cagttccctg ctccgaaccg acataagcaa agacacaaaa gaagaaagcc
      121 cacaccatgg gcttgcggaa caagcttccg tacatttccc aggatccagc ctgctcttct
      181 gccgtgtgtt cgacctgcgg gaaacggctg aagtagagga ccacgatcat caacaaagtg
      241 aaaacagcga agatccagta catggacgcc cagggaagag ccggtggggt cagccgtctt
      301 aggactgtga gcaggaggtt cctgtgggcc gaaaaatccg ctagattcac caccaagtat
      361 gagtacacgt aggggctgag aaatgaggct gagccgaaga tcagttgtgc caaggcggaa
      421 ttgaaagcga aatgctcctc ccctccagcc acgcgcagca gcggattgat cgccgtctgc
      481 aaggtggcca tgcccgaccc catgacaaac aacgaggcca cagccacgcg atagctagga
      541 tgtgcggcga gactcaacga ccccgcggtg ccggccaaga acgcggcgat catgaccggt
      601 ttctcagtga aacgctcgac gaggaatccc gcaggaatcg acatcactcc gtaagcgata
      661 aagaaagaga atggtaagaa ggccgccgct gcaagcccga cgtgaaaact gctgatgatg
      721 tcgggcacga gcgcgttcag aatattcgtc aacgcggaca ttacgaagaa tgtcaggaag
      781 acaatgacga ccatgtatcg gtttcggggc atggaacgcg acatcttctt ctattccctc
      841 gtcgtaactt tcgaagccag ttcgaagcgg ccgggctcgc ggcctacctg cgccgccaga
      901 gcgagcgtga ccatctgcac cggcagaatt tcgaggatcg ggtggatcga gcgcggcgcg
      961 gcgggcaaca cgcaagcgga agacgcggag tcttcgctta tcatctccgt ccgtccctgc
     1021 tgctcgcgca catcatcgaa gagccgctgg ttcagatcgc gagtcttgtt ctcgccggcg
     1081 aagacgagta caaaagtctc gtcgctcagc atctcgagcg ggccatgccg aaatgccgcg
     1141 ctgctcatgc cttctgcatg aaagtggtcc gcctctttta cgatcagcgc gcctgtgcca
     1201 accgccgcca gtgaagcccc gcgtccgacg agaaacaggt ggcggatgcc ctcgagcagc
     1261 tgacccagct gctgcacatg ttctttccag tgcttcaggt attcgtcaac ggcaggcccg
     1321 gcagccttga gttcctgtcg agtgcgacgc aactcacgtc cggatacgac atcgccaagc
     1381 cattgcaatg ccatgagagc cgacaggtag gtcttgcagg aaactgagaa ctccttgccc
     1441 gcgcgtgtca agatcgtagc gtcggcgcgc tcagctagag ggctatcagg cgtgttggta
     1501 atcgcgataa tcgatgaccg gccgcgattg atctccagca accggactac ctccgcactc
     1561 tgtccggatt gagaaactgc cacgatcagg gtttggtgat ggaacagccg gctccgataa
     1621 tgcagaagtt ctgaagtctc gaccatcatt gcggtcaggc cgtggttgat gagttcgatg
     1681 ttgagaggat gcagggcgtg gaaggacgag cccatgcccg tgagcacgac cgtcttgaac
     1741 tttcccttct gcaggcgggc cgcgagttcg cgcagaggct tcgtaacctc aagcccttcc
     1801 agcgtctggc tgagagcgcg aggctggtcc agcagatcgc gaagatattc gccgtcgatg
     1861 acggaaaact cagacagcat ttttctccgc ctccgatccg cagcggtaat accagactct
     1921 tccggctccg atcaggttgg tatcgctccc cagcgaggcc aaggccagct ccgtcttctc
     1981 atagggcaca aaacggcagc tggaggcgat caccttgcga atgccttcca taaacaacga
     2041 agcgctctgc ataacgctgc cgccaaggac gatgatctcg ggaacaaaca tgaccaccag
     2101 attggctaga cccaggccga gatagcggct ctcgtgctcg atggcccgca cggcccacgc
     2161 atcgccttct tgagccaatt cgcagattct ctttgccgta aggccaatcc ggtggtggta
     2221 gtcgaggggc gcgttgcgct cgatccactt gaccatcgac ggcccggccg ccagcgactc
     2281 ccagcaacca tggaagccgc aatcgcagag tggcccagat ggatcaatga gatgatgacc
     2341 gatctccgga tgtgctcgat cgacgccacg atagacccgg ccctcaagaa tgattccgac
     2401 tccaatgccc gtaccgaccg tgacataaat cagacttttc ctgccgcgtc cagcgcccca
     2461 gcctgcctca cccacggccg cagcatcggc atcgttttcc atcgccacag agacgtggaa
     2521 gatctgttct aagtcccgg
//
LOCUS       DASPUL010000581        12110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_519726, whole genome shotgun sequence.
ACCESSION   DASPUL010000581 DASPUL010000000
VERSION     DASPUL010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 12110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12110)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12110
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519726"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /gene="hemA"
                     /locus_tag="VEJ00_13490"
     CDS             complement(<1..1077)
                     /gene="hemA"
                     /locus_tag="VEJ00_13490"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HXX02224.1"
                     /translation="MEPTLMVIGLNHRTAPLAMRERFWIGENQRYQALRRLKSAEGID
                     EVVVVSTCCRTEFLVWAGEPTLAANSLLQFLSAEHGLRLREWEHFYRLLDEAALTHIF
                     RVASGLDCLALGEPQIATQISAALQQAQTLGACGRFLNAVVEKAVSVACQVRQEAAID
                     KLAVSLPAAAYELALHIFGSLENRRILLLGSGQAGELCARYMVEKDAGSVTVIDQSQP
                     RAQELAQKMGATAAPLTDRWKYMLRADIVISSTGCPHVVLTAEEAERIAQERNRVALL
                     ILDIAMPRDVDPEVRRVDGILLYDFDGLERMMKCHAVEHPEAVLKAERMVAAEAQAFR
                     GALQAESVVPTIVALRHRLDEICRQ"
     gene            1446..1781
                     /locus_tag="VEJ00_13495"
     CDS             1446..1781
                     /locus_tag="VEJ00_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXX02225.1"
                     /translation="MKTMKLMLVIVMIALALFLVLPNLSWAADDGATLYKTKCAACHG
                     ADLAGKPAAKIPSLVSDEAKKASDADLTDMIANGGKDKKAMHAFANKGMTPDQVKMVI
                     AYVRDAQKK"
     gene            complement(2028..2885)
                     /locus_tag="VEJ00_13500"
     CDS             complement(2028..2885)
                     /locus_tag="VEJ00_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02226.1"
                     /translation="MKQSSVKVCPRSTRSLLAGTALVLCATLNGCIGATPLPKRTRTA
                     TGVEVKTVDLTFIHPGQTTRDEVREKLKVIDTGYVGDRYFLGRWSSSTWGGWIIAAGM
                     CCEAAGGGGRVWKTGNLLVEFDDAGLVKRAEAFNDRKVLQELTPVAENAPLQLDPPVE
                     LRLKYWNGGTAPVAAKITLTPARLDVEELDDSSDNDQKKNGVKPGKQKKLARFSVPAN
                     NLRKVEMPYAQGDDPTLLGPRIHCAVDLRYFGGPRLKYLNLQVTLPQTVTLMSYITQV
                     KAAAEPDAK"
     gene            3082..4188
                     /locus_tag="VEJ00_13505"
     CDS             3082..4188
                     /locus_tag="VEJ00_13505"
                     /inference="COORDINATES: protein motif:HMM:TIGR04053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04053 family radical SAM/SPASM
                     domain-containing protein"
                     /protein_id="HXX02227.1"
                     /translation="MVVAGTTPKMGHVAHDFSRNPMLVYWEMTQACGLACKHCRAEAM
                     PTANPLELTTEQSKRFLNQLVDFGDPLPHLILTGGDPLSRRDIYSLIDYANGLGLEVS
                     ITPSATPELTNEAISKLKEHGIQSMGLSLDGSCAEKHDNIRAVPGTFERTMEAARHCG
                     RIGLPIQVNTLVAEETADDLPAIYELLRTSFPVMRWSLFMLISVGRGKQLNEVSPEEG
                     ERIMKWVFELSMHSPFQVKTTEAPSYRRIAIETMRGSGMAKPEMKATSVYKGFQIRDG
                     HGIVFVSNLGEIYPSGFLPLRCGNVRTDSLVEIYRNSDIFRALHSPDTFHGKCGACEY
                     SHICGGSRARAFAYTGDALGTDPFCPYEPAVAQC"
     gene            complement(4381..4698)
                     /locus_tag="VEJ00_13510"
     CDS             complement(4381..4698)
                     /locus_tag="VEJ00_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02228.1"
                     /translation="MIHLSIMAGSVFAKSLGRIDAVAPDKLLFSAAKSTNPLGKYYLA
                     EIPLSGGEFEYLDAKDAAEPQRSRRKGYEALLHIRFRDAVGKLQIAIGFAVVPPFDEW
                     VKL"
     gene            complement(4880..6073)
                     /locus_tag="VEJ00_13515"
     CDS             complement(4880..6073)
                     /locus_tag="VEJ00_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02229.1"
                     /translation="MGNPLRTLLDLPEQEKSDRGLEHTPREIWQQPATWRDTWQRCFD
                     RQPELQDALRRAGIGRGSTSSPTVYLLGAGTSDYIGRALAPLLRRQWGCDVWAIPSTT
                     LLTEFEEFHRPGREYLWISFSRSGDSPEGVALLERALDRDREIRHLVITCNQQGTMAQ
                     LCEQHPDRALALVLGDAVNDRALAMTSSFTNMLLAGQCVGHLEEFTEFGKVVAQVVDA
                     GQQFLPQAAEVAAAITMLGCTRACFVGSGALGAVAAESALKVVELSAGKVSTISETTL
                     GLRHGPMSSVDGQTLFVAFLSSDARRRGYELDLVREIDRKRLGRVRAVVSVQGIDDVA
                     SLADYTLALQCPPDFPDYYRPVLDVMFGQLIGLFASMRCGLKPDQPSPNGAITRVVQP
                     IKLYS"
     gene            6320..6595
                     /locus_tag="VEJ00_13520"
     CDS             6320..6595
                     /locus_tag="VEJ00_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02230.1"
                     /translation="MSSGSHPLRSLLLALALTSSVAGIACEHHYYRVYDPYYTDYHVW
                     NDDEIVYYHRWADETHRDRNRDFRRLSKDEQKEYWTWRHNHGDHDHH"
     gene            complement(6735..7364)
                     /locus_tag="VEJ00_13525"
     CDS             complement(6735..7364)
                     /locus_tag="VEJ00_13525"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HXX02231.1"
                     /translation="MRSRDFIFWEVDCQADFMLPGGKLYVPGAEKLLPNIKRLTDAAR
                     EGRVFLVSHGCFHTPNDPEFKVFPPHCVKGTAGAELVPEALTEKVARVHNDPDAKLPE
                     DLSRYQQILLEKQTLNIFESRHADALVQRLGTNPEFVVFGVVTEYCVSFAVQGLRQRG
                     RRVAVVRDAIETLKPEDGATALAEFERLGARLTTTDQALRDLGRSEPPD"
     gene            7439..8443
                     /locus_tag="VEJ00_13530"
     CDS             7439..8443
                     /locus_tag="VEJ00_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXX02232.1"
                     /translation="MQNHRSDSTSQHPWGVLCAVATAILVMLSLTAISPLLVAQNTVP
                     QWCRPLPRPEYKALERVLISDPWFEVYRPTRGVFAIYEPHQSEEVISYLIVGEKRALL
                     FDTGMGISDLKKLTPELTKLPIIVLNSHTHDDHVGDNWEFETIYGMNTDFTRANARGS
                     REDAQAEIAPGEICGTLPKGFDAEAYATRPWKITAYTHDGDRFDLGGRVVEVIATPGH
                     TPDAISLIDRGNGLLFTGDTYYPAPIWLFRPETDLDAYAASIRRLAALAPEVKVVLGA
                     HNIPVASPSVLARLVAAFDVVRAGKVAATPDSPGKVLYKVDDISFLMRALDTTNHHKP
                     "
     gene            8440..9873
                     /locus_tag="VEJ00_13535"
     CDS             8440..9873
                     /locus_tag="VEJ00_13535"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXX02233.1"
                     /translation="MSAADNPSLQAAEAEVQAHGGGLRRELGMVDLVFAQILLVIVPD
                     FFGTAVKAGPPHVVYWLAAILLFFIPLAMVVAHLNQLMPLEGGLYEWARLAFNDQIGF
                     LVAWNLWLFVILYVAVIGVVTVSFVTYVIPSVAWIAANKGLIVAASFVWMAVMALIAA
                     RGLQLGKWVSNAGGIAILITIAVLVLAPWLNLWRGTLTEFHPLRLVEPATTLFSLSVF
                     SKLSFGALSGFEYIAIFAGECRSPRRHLTRSILITAPLIALIYIFATSSILAFVSPES
                     VDVVAPIPQALSLGSRAFGLAKIVLPLAIISLFINYPTTFSTYFSGSVRLPMAAGWDH
                     LLPEWFSRLHRVYKTPVNSILLVAAATLAASVAALIGVGPQETYELLLTWGFTFYALA
                     YLALFAIPFLSPKGRGLRPGLWLRLAAVSGFLMTLLYVVLSVFPIIDVDSSSRYSLKV
                     AAVVLGANLLGWMIYRAGQRKTAGDSA"
     gene            complement(9865..11124)
                     /locus_tag="VEJ00_13540"
     CDS             complement(9865..11124)
                     /locus_tag="VEJ00_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02234.1"
                     /translation="MRKLSLVATIALLVSPLLAQGKHLWVLRAPGEMVEYDPATFAVK
                     QTVKVPPEAVRSPDSVQLNRPGEILFAPAVSLPLAERDVASPHKAWIWNGHAATTIDL
                     GLKRETAATGSNQAVTESAPAAYLSADGGHLYWFANHARRLQREDVDLSITTTWQAWQ
                     SDLNGAGRRDIATVNLPDCRCSSGACEETCPSGVVWVPDGGVDKFFVMTQFFAGQTEV
                     VFKASTRYREEAGKWTADALTDPLQRVLDATSDGNVIVDAIPDTGCCGWSNESNDQTV
                     VRKGDKRLTVFDEFATYRNSDYDVSFYTGNARLSPDLSLVAMTIVATAEPNEPIQLSD
                     QGQANPEESKEIRKVLAELPAVEVKSLDDSPRRIAFVPHATLVGWVSEKEILIVEDHL
                     LVAYNVSTGTRRMSIIRVEDAAHVFLR"
     gene            11268..>12110
                     /gene="hemQ"
                     /locus_tag="VEJ00_13545"
     CDS             11268..>12110
                     /gene="hemQ"
                     /locus_tag="VEJ00_13545"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: protein motif:HMM:NF008913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HXX02235.1"
                     /translation="MTTMHPPRSETSVELPAVPLTTEGYSVLHQMMRFRRSAWRALPA
                     SEKSAIAGEAASLLSAMEANSSGQSALYSLIGHKGDLMLIHFRESFAALNLAELKLAN
                     LRLSDYLEPTTSYLSIIELGLYDSTLKIYKELRDQGIEAHSDQWKAEVECKLARHKEA
                     MHPRLFPQIPPNRYACFYPMDRRRGEDKNWYTLPIEERARQMNEHGLVGRRYAGEVKQ
                     IITGSIGFDDWEWGVDLFADDPLVFKKLIYEMRFDEVSAVYALFGQFYVGVRVPAAAL
                     EKLLA"
BASE COUNT         2394 a         3598 c         3676 g         2442 t
ORIGIN      
        1 ttgccggcaa atttcgtcca ggcgatggcg caacgccacg atcgtaggca cgacactctc
       61 ggcctgcagt gcgccacgaa aggcttgcgc ctcggccgca accattcgct ccgccttcaa
      121 gactgcctcg ggatgctcca cggcatgaca tttcatcatg cgctcgagac catcgaagtc
      181 gtagagcaga atgccatcca cccggcggac ttcaggatcc acgtcgcggg gcatggcaat
      241 gtcgaggatg agcagcgcta cccggttccg ctcctgggca atccgctcgg cttcttcggc
      301 ggtgaggacg acgtgcggac atccggttga gctgatgacg atgtcggcgc ggagcatgta
      361 tttccagcga tcggtcagcg gggcggcagt ggcgcccatt ttctgggcaa gttcctgggc
      421 gcgcggctgc gactggtcga tcacagtgac ggatccggca tccttctcga ccatgtaacg
      481 agcgcagagt tcgccggcct gtccactgcc cagcaacagg attctgcggt tctcgagcga
      541 gccgaagatg tgaagggcca gctcatacgc ggccgcggga agcgagaccg cgagtttgtc
      601 aatcgccgcc tcctgccgca cctgacaggc aacgctgacc gccttttcta ccaccgcatt
      661 caggaaacgt ccgcaagccc ccagggtttg tgcctgctgc agggcagcgc tgatctgagt
      721 tgcgatctgc ggttcgccga gcgccaggca atcgagtccc gaggcgacgc ggaaaatatg
      781 ggtgagagcg gcttcatcga gcaggcggta gaagtgctcc cactccctca gtctcaagcc
      841 gtgctcggcg ctgaggaact ggagcagtga attggcggcg agcgtgggct cgcccgccca
      901 taccagaaac tcggtgcggc agcaggtgga aacgacgacg acctcgtcaa tgccttccgc
      961 gctctttaac cggcgtaagg cctggtagcg ctggttctca ccgatccaga agcgctctct
     1021 catggcaagc ggtgcggtgc ggtggttgag cccgatcacc atgagtgtag gttccatgac
     1081 aacacctcgc gtgcaggtcg catgactcaa agggcacgcc ccgtgccaaa gaatcgcggg
     1141 gaatctgcag cgtcaatggt tttgttttca ggggcttggc ggagcgacgg agaggttcga
     1201 gcgtgggtcc gaccgactgc ggtgcggcat atgacgcgcg aatccgagtc ttttgacgcg
     1261 atcgcggctg cgggccatct cgaaggggca caaggagttg gagccgcgtc acgatcacga
     1321 ttcagcgtga tcgtaatcac tgaccactcc cccaacctcc gtagaaactg ctgaacgatt
     1381 agcaggctct tactatcgag tcgcggctcg ttcccgactc gaagctgtga aaggaaaaag
     1441 caatcatgaa gacgatgaag ctcatgctcg tgatcgtaat gatcgcgctg gccttattcc
     1501 tcgtgttacc gaatctgtcc tgggccgccg atgatggcgc gaccctttac aaaaccaagt
     1561 gcgccgcctg ccacggcgcc gacctcgccg gcaaaccagc tgcgaagatt cccagcctgg
     1621 tttccgatga agccaagaaa gcctccgacg ccgatttgac cgacatgatc gccaacggag
     1681 gcaaggacaa gaaagccatg cacgcgttcg ccaacaaggg catgacgccg gatcaggtca
     1741 agatggtcat cgcctacgtg cgcgacgcgc agaaaaagta gcggagcggt caagaccgct
     1801 gttccgagag gcgaaccgcg cgggaccccg gacgcagttg gcgcgcgagc atgcgagagt
     1861 gagtgttccg ctcaccggcg gaatccggcg acgaagccgg atagccaggg aactcctctc
     1921 cgacacgcca gcacacggaa cagccagccc gggtctaggt gctcaccccc agacccgggt
     1981 atttgtttct aagcagcgcc agaggtcaga cgcttctctg cggcgcctca cttcgcatca
     2041 ggctccgcag cagctttcac ctgcgtaatg taactcatca gggtaaccgt ctggggcaga
     2101 gtcacctgca ggttcagata cttcaagcga ggccctccga agtaacgtaa atccaccgca
     2161 caatggattc tcggtcccaa caacgttggg tcgtccccct gtgcgtaggg catttccacc
     2221 ttccgcaggt tgttcgcggg cacggagaac ctggcgagct ttttctgttt gccaggtttg
     2281 acgccgttct tcttttgatc gttgtcgctg gaatcatcca gttcttccac gtcgaggcgg
     2341 gcaggagtta gggtgatctt ggctgcaacg ggcgccgtac caccattcca gtatttcaga
     2401 cgcaattcga ctggcgggtc gagttgaagg ggcgcgttct ccgccaccgg cgtcagttcc
     2461 tggagcactt tccggtcgtt aaacgcctcc gctcgcttta cgaggccagc gtcgtcgaac
     2521 tccaccagca gattgccggt cttccacact cttcctcccc cgccggccgc ttcgcagcac
     2581 atccctgccg cgataatcca accaccccag gttgacgagg accagcggcc caggaaataa
     2641 cgatccccga catagccggt gtcgatgact ttgagtttct cccggacttc gtcgcgggta
     2701 gtttgacctg ggtgaataaa cgtgaggtca acggtcttga cctcaactcc tgtcgccgtg
     2761 cgcgtgcgct tgggcagcgg cgttgcgcca atgcaaccgt tcagagtggc acagagaacg
     2821 agggcagtcc cggcaaggag cgatcgggtc gaacgagggc acaccttcac gctcgactgt
     2881 ttcatggaat gcacccccaa tgggcggcaa ttgtacgcct ggaggcacga tttccgggaa
     2941 aaagtgccta aatcctgtgt tcttagcgaa gggcgtcacg agcaggtgtg atcgtaatca
     3001 cagccttttt gtactaggaa acgtagtctg aagctgcttg tgggtgttct ttcataagtt
     3061 cttccgccga aaggggacat catggtggtc gccggcacta ctccgaaaat gggccacgtg
     3121 gctcacgact tttcccgaaa tccgatgctg gtctactggg agatgaccca ggcatgtgga
     3181 ctggcttgca agcattgccg cgccgaggcc atgcccactg caaatccgct ggaacttaca
     3241 accgagcaaa gcaagcgctt cctgaatcaa ctggtcgatt ttggcgatcc gctgccgcac
     3301 ctgattctga ccggcggcga tccgttgagc cgcagagata tctactcgct cattgattac
     3361 gcaaacggac tgggtctcga agtgtcgatt actccgagcg ccacgccgga attgacgaac
     3421 gaagccatct cgaaattgaa agagcacggc attcaaagca tggggcttag tcttgacggc
     3481 tcctgcgcgg agaagcatga caacatccgc gcggtcccgg gaacgtttga gcgcacgatg
     3541 gaagcagcgc ggcactgcgg acgcatcggg ctccccatcc aggtcaacac gctggtggcc
     3601 gaagagacgg ccgacgatct gcctgcgatc tacgaactgc tccgcaccag ctttcccgtg
     3661 atgcgctgga gcctgttcat gctgatctcg gtcggccggg gcaagcagtt gaacgaggtt
     3721 tcgcctgaag aaggcgaacg catcatgaaa tgggtgttcg agctctcgat gcattcacca
     3781 tttcaggtga agaccacgga agcgccgtcg taccggcgca ttgcgattga aacgatgcgc
     3841 ggctccggca tggccaagcc ggaaatgaag gcgacatcag tttacaaggg attccagatt
     3901 cgcgacgggc acggcattgt ttttgtgtcg aacctgggcg agatttatcc gtcaggattc
     3961 ctgccgttgc gctgcggcaa tgtgcgcacc gattcgctgg tggagatcta ccgcaactcc
     4021 gacattttcc gcgctctgca ctcgcccgac acattccacg gaaagtgcgg cgcgtgcgaa
     4081 tacagccata tctgcggtgg atcgcgggcc cgcgccttcg cgtatacggg tgacgccctg
     4141 gggacagacc cgttctgtcc gtatgaaccg gccgtggcac agtgctaggt gcagcaaaag
     4201 gaagaagact tgggggcttc cccgtctcaa atcaggtaca ttcaggcgca tgaattggaa
     4261 aaacagtctt cgcgaagaaa catgagagcc aaaggaaaag gcgtgaccgg gaagcgccaa
     4321 cagccgccgc cagatgagtc gaagaacaag atccttgagg gactgcctca actcggccgg
     4381 tcagagctta acccattcgt cgaatggagg gacgacggcg aagccgatcg cgatctgcag
     4441 tttcccgacg gcatcgcgaa atcggatgtg gagtaaagct tcgtatccct tgcgccgcga
     4501 gcgctgaggt tcggcagcat ccttcgcatc caggtattca aactcaccgc cggatagtgg
     4561 aatctcggca aggtagtact ttcccagcgg attggtcgac tttgcagcgc tgaagaggag
     4621 cttgtctgga gcaacggcgt caattcttcc cagcgatttg gcgaagacgc tgccagccat
     4681 gatcgaaaga tgaatcacgc gatcttcgtt tttccatttg ttgagcagat cgacggcctc
     4741 atcggacgac agccttacct cgggacttat actgcggcga aattatacgc ctggcccaag
     4801 cgccgaacct gccgctgatg cccgcaccgg caagattccc tgcctcatac gcgctccaaa
     4861 tgtgtgctga ggaaacccgt caggaataca gcttgatggg ctggacgacg cgtgtaatgg
     4921 cgccgttcgg gctgggctgg tcgggcttga ggccgcagcg catggaagcg aacaggccga
     4981 tcaattgccc gaacatcacg tcgagcacgg gacgatagta gtcggggaag tcgggcgggc
     5041 actgtaacgc cagagtatag tccgccagcg atgccacatc gtcgatgccc tgaacgctga
     5101 ccacagcgcg aacgcggccc aggcgcttgc ggtcgatttc gcgcacgaga tcgagttcgt
     5161 aaccgcggcg gcgggcgtcg ctggagagaa aggcgacgaa cagggtctgt ccgtcgacgc
     5221 tcgacatggg gccgtggcgc agtccaagcg tggtctcaga tatggtacta accttgccag
     5281 cgctgagctc caccactttc agcgccgatt ctgcggcgac ggcgccgagc gctcccgatc
     5341 ctacaaagca cgcccgtgtg cagcccagca tagtgatggc ggcggcaacc tcggcagctt
     5401 gggggaggaa ttgctggccg gcgtcgacca cttgcgcgac tactttgccg aactcggtga
     5461 actcctccag atgccccacg cactggcccg ccagcagcat gttggtgaat gagctggtca
     5521 tggcgagggc gcgatcgttc accgcatcgc cgagaaccag agccagggcg cggtcgggat
     5581 gctgctcgca caactgcgcc atcgtgccct gctggttgca ggtgatcacc agatgccgga
     5641 tctcgcgatc ccggtcgagc gcccgctcga gcagcgcgac gccctccggg ctgtcgccgg
     5701 agcgggaaaa cgaaatccac agatattccc tcccggggcg atggaactct tcgaattcgg
     5761 tgagcagagt cgtgctggga atggcccaga cgtcgcaacc ccactgccgg cgcagcaacg
     5821 gcgcgagagc acggccgatg taatccgacg taccggcccc gagcaaatac actgtgggcg
     5881 aggaagtgct accgcggccg atcccggcgc ggcgcaaggc atcttgcagt tcgggctggc
     5941 ggtcaaagca acgctgccag gtgtcgcgcc aggtcgcggg ctgctgccag atttcgcggg
     6001 gcgtgtgctc gaggccgcga tcgcttttct cctgctccgg caggtccagc agcgtgcgca
     6061 aggggtttcc caaacggcga tctcctcggt caaagacact gtagtctatt acaatccgag
     6121 tctgttgaaa agcattcgaa agtaaatgaa attgcgttcg gaagggaacg tcagagacgg
     6181 actggtcgct gcgctgcatg aagttcctct tcgatggagt tgattctacg gatacttccc
     6241 ggacggagtt gcaacccctg gccaggccgc ggcaactaat gccgctgccc tgtgttagat
     6301 tcggcggaag gagaacaaca tgagttctgg atcgcatccc ctgcgttcgc tgctgctggc
     6361 gctggcgctg acatcgtcag tggcagggat cgcctgcgag caccactact atcgcgtcta
     6421 cgatccctac tacaccgact accacgtctg gaacgatgat gagatcgtct actatcaccg
     6481 ctgggccgac gaaactcacc gcgatcgcaa ccgcgatttc cgcagactgt cgaaggatga
     6541 gcagaaggag tactggacgt ggcgccacaa ccacggcgac cacgatcatc actagacccg
     6601 aggcggctaa agccgcggtt gactggtggg atttcggtgc ggctggaagc cgcacccttt
     6661 caaatcagac gtgtcgcccc aggaactctg gcaagcggct ctgagctggt tcttttttca
     6721 gcgatccgcg ggggtcaatc gggcggctct gagcggccga ggtccctgag agcctgatcg
     6781 gttgtggtca gacgcgctcc taggcgctcg aactcggcga gggccgtcgc gccgtcttcg
     6841 ggcttaaggg tttcaatggc atcgcggacc accgccacgc gccgtccgcg ctgtcgcaaa
     6901 ccctgcacgg caaagctcac gcagtattca gttacgacgc cgaaaacgac gaattcggga
     6961 ttggttccca gcctctgaac aagagcgtcg gcgtgccggc tttcgaaaat gttgagggtt
     7021 tgcttctcga gaagaatctg ctggtagcgc gacaggtctt ccggcagttt cgcatcagga
     7081 tcgttgtgca ctcgcgcgac cttttcggtc aaggcctccg ggactagctc cgctccggca
     7141 gttcccttga cgcagtgtgg cggaaagacc ttgaattcgg gatcgtttgg tgtgtggaaa
     7201 cacccgtggg agaccagaaa tacgcggcct tctcgcgcag cgtctgtcag acgcttgata
     7261 ttgggcagga gtttttcggc gcccggcaca tagagcttgc cgcccggcag catgaagtcg
     7321 gcctggcaat ctacctccca aaagatgaaa tcgcgagagc gcatgaattt ctccggtgcc
     7381 cggatcgagc gtaacactgc cgcttctatc gtgacaaagc gtcgcgtatg atttgcggat
     7441 gcaaaaccac agaagcgatt ccacaagcca gcacccgtgg ggagttcttt gcgcggtggc
     7501 aacggcgatc ctggttatgt tgtcgctcac cgcgatatcg ccgctgctgg tcgcacagaa
     7561 taccgttccc cagtggtgcc ggcctctgcc gcgcccggag tacaaggcgc ttgaacgcgt
     7621 cctcatcagt gatccgtggt tcgaagtgta ccggccgaca cgcggcgtct tcgccattta
     7681 cgagccccac cagtcggagg aagtcatcag ctacctgatc gtcggcgaga agcgggcgct
     7741 gctgttcgat acaggcatgg gcatcagcga tctgaagaaa ttgacaccgg agctcacgaa
     7801 gctgccgatc atcgtgctga attcgcacac ccacgacgat cacgttggag acaactggga
     7861 attcgagacg atctacggga tgaatacgga tttcacgcgg gcgaacgcac gcggttcgcg
     7921 cgaagacgcg caggccgaga tcgcgcccgg ggagatttgc ggcacgttac ctaagggatt
     7981 tgacgccgaa gcctatgcga cgcgtccctg gaaaattaca gcttacacgc atgatggcga
     8041 tcgcttcgat ctcggcggac gagtcgtgga agtcattgcc acgcctggtc acactcccga
     8101 cgcgatttcg ctgatcgacc ggggtaacgg gctgctgttc acgggagaca cgtactatcc
     8161 cgcaccaatc tggctcttcc gcccggagac ggatctcgac gcttatgcgg catcgattcg
     8221 gcggctggcg gctctggctc cagaggtcaa ggtggtgctg ggagcgcaca acatccccgt
     8281 cgcgtcaccg agcgttctgg cccggctggt agccgcgttc gatgtggtac gcgcgggcaa
     8341 ggttgcggcg accccggatt ctccaggcaa ggtgctgtac aaggtggacg acatttcatt
     8401 cctgatgcgg gcactcgaca cgacgaatca ccacaaacct tgagcgccgc cgacaatccg
     8461 tcgctgcagg cagccgaggc cgaggtacag gcgcacggcg gagggctgcg ccgggaactc
     8521 ggcatggtcg atctggtttt tgcacagatt ctcctcgtca tcgtgcccga cttcttcggc
     8581 acggcggtca aggcgggccc gccgcatgtt gtctactggc ttgctgccat cctgcttttc
     8641 tttattccac tcgccatggt ggtcgcccat ctgaatcagc tcatgccgct ggagggcggt
     8701 ctgtacgaat gggcgcgcct cgcattcaac gatcagatcg ggtttctggt ggcctggaat
     8761 ctgtggctgt ttgtgattct ctatgtcgcg gtgattggag tcgtgaccgt ctcgtttgtc
     8821 acatacgtaa ttccgagtgt ggcgtggatc gcagcgaaca aggggctgat tgtggcggct
     8881 tcgtttgtct ggatggcagt tatggcgctg atcgcggccc gagggctgca actggggaag
     8941 tgggtcagca acgccggggg aatcgcgatt ctgatcacca tagcagttct ggttctcgcg
     9001 ccctggctga acctgtggcg cggcacgttg accgagtttc accctctccg gcttgtcgag
     9061 ccggctacta cgcttttcag cctcagcgta ttcagcaaat tgagtttcgg cgccctcagc
     9121 ggtttcgaat acatcgcgat cttcgccgga gaatgccgca gcccgcggcg gcatctgacg
     9181 cgatcgattc tgatcacggc gccgctgatc gcattgatct atattttcgc gaccagttct
     9241 attctcgcct tcgtttctcc cgagtcggtg gacgtggttg cgccgattcc tcaggcactc
     9301 agcctcggtt cccgggcatt cggcctggcg aaaattgtcc tacccttggc gatcatctct
     9361 ctcttcatca actatcccac cacattcagc acctatttca gcggaagcgt gcgcctgccc
     9421 atggcagcag gatgggatca tctgctacca gagtggttct cgcgcctgca ccgcgtctac
     9481 aaaaccccgg tcaactcgat tctcttggtg gctgcggcga cgcttgcggc aagcgttgcc
     9541 gccttgattg gcgtcgggcc gcaggagaca tatgaactgc tcttgacctg ggggtttacg
     9601 ttttatgccc ttgcctacct tgcgttgttt gctattcctt tcctgtcgcc gaagggtcgc
     9661 ggacttcgac ctggcctctg gctgcggctg gctgccgtgt cgggctttct catgacgctg
     9721 ctgtacgtgg tactgtcggt tttcccgatc atcgacgtgg acagctcgtc gcgctattcg
     9781 ctcaaggttg cagcagtcgt gctgggggcg aacttgctgg gctggatgat ctacagggca
     9841 ggtcaacgaa agacggcggg cgattcagcg taggaacacg tgcgcggcat cctccacccg
     9901 gattatcgac atacgccgcg ttccagtcga cacgttgtac gccacgagca gatgatcttc
     9961 tacgatcagg atctccttct cgctgaccca gccgaccagc gtggcgtgcg ggacgaaggc
    10021 gatgcggcgc ggagaatcgt ccagactctt gacttcgacc gccggcaatt ccgccagtac
    10081 cttgcgaatt tcctttgact cctccggatt ggcctgcccc tggtcggaca gttgaatcgg
    10141 ctcattgggc tctgccgtag cgacgatggt catggccact aaactcagat cgggtgacag
    10201 gcgcgcattt ccggtgtaaa aactcacatc gtagtcggaa tttctgtagg tggcgaactc
    10261 gtcgaagacg gtgaggcgtt tgtcgccctt ccgcaccacg gtctggtcgt tgctttcgtt
    10321 cgaccagccg cagcaaccgg tgtcgggaat ggcatcaacg atgacgttgc catctgatgt
    10381 tgcgtcgagc acgcgctgga gcgggtcagt gagtgcgtcc gccgtccact tccctgcctc
    10441 ttcgcggtag cgagtgctgg ccttgaagac cacttccgtc tgtccagcga agaactgggt
    10501 catcacgaag aatttgtcca caccgccatc aggtacccaa actacgccgg aggggcaagt
    10561 ttcctcgcag gctccgcttg aacagcggca gtcgggcagg ttcacggtgg cgatgtctct
    10621 gcgacccgcg ccgttgaggt cgctctgcca tgcctgccat gtcgtggtga tcgagagatc
    10681 gacgtcttcg cgctgcaagc ggcgcgcgtg attggcaaac caatagagat gcccgccgtc
    10741 tgctgaaaga taagccgcag gcgcggactc ggtgaccgcc tggttcgatc cggtggcggc
    10801 agtctcgcgc ttcaggccca ggtcgatcgt tgtggcggcg tgcccgttcc agatccacgc
    10861 cttatgcgga gatgccacgt cgcgttcggc gagcggaagg gagacagccg gcgcaaacag
    10921 aatctcaccg ggacgattta gctggacgct gtccggcgac cgcacggctt ctggcggaac
    10981 tttcactgtc tgctttacgg cgaatgtcgc cggatcgtat tcgaccatct ctcccggggc
    11041 gcgcagcacc cacaggtgct tgccctgggc cagcaggggc gagaccagca gtgcaatggt
    11101 ggcgactaga gacaatttgc gcatggacta tttatatcgc agagacggtg cagctacttc
    11161 tgtaagccac cgcaaacggc ccacttctcg caaaagcggc gagaagtggg gcaccccaca
    11221 acatgtctga gtatcccgat tcctccgggg tacaatgaag gctcgctatg actacgatgc
    11281 atccgccccg ctccgaaacg tccgtcgaac tccccgcagt tccgctcact accgaaggtt
    11341 acagcgtcct gcatcagatg atgcgcttcc ggcggtcggc ctggcgagcg cttcccgcaa
    11401 gtgagaaatc cgccattgcc ggtgaagcgg cgtcgctgct gtcggccatg gaggcgaact
    11461 catcggggca atctgcactc tactcactca tcgggcacaa gggcgacctg atgctgattc
    11521 acttccgcga gtcgttcgcc gcgctgaatc tggcggaact caaactcgcc aatctgcggc
    11581 tgagcgatta tcttgagccg acgacatcgt atttgtcgat catcgaactc gggttgtacg
    11641 actcgactct caagatctac aaggagctgc gcgatcaggg catcgaggcg cattcggacc
    11701 agtggaaggc cgaggtcgag tgcaagctgg cccggcacaa ggaagcgatg catccgcggc
    11761 tttttcccca gatcccgccg aaccggtatg cgtgttttta tcccatggac cggcgccgag
    11821 gcgaagacaa gaactggtac acgctgccca tcgaggaacg cgcgcggcag atgaacgagc
    11881 acggcctggt aggccggcgg tacgcgggcg aggtgaagca aataatcacc ggttcgatcg
    11941 gattcgacga ctgggagtgg ggcgtcgacc tgttcgccga cgacccgctg gttttcaaga
    12001 agctgatcta cgagatgcgc ttcgacgaag tcagcgccgt ctatgcgctg ttcgggcaat
    12061 tctatgtggg cgtgcgggtt ccggcggcgg cgctggaaaa actgctggcc
//
LOCUS       DASPUL010000582        11740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1064140, whole genome shotgun sequence.
ACCESSION   DASPUL010000582 DASPUL010000000
VERSION     DASPUL010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 11740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11740)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11740
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064140"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..2087)
                     /locus_tag="VEJ00_13550"
     CDS             complement(177..2087)
                     /locus_tag="VEJ00_13550"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HXX02236.1"
                     /translation="MDDEKRDRELGMATRITRRDFLNGMAVAVGALGSAGIADFARAA
                     QSTGQSDGHPAPSYPPGLTGMRGSQPGSFGVAHSLRDGTFWETAGTPEDTHETYDVVV
                     VGGGISGLAAAYFYRKHAGKDARILILDNHDDFGGHCRRNEFQVDGRLLLSNGGTQSI
                     ESPSEYSEVSKSVLKDLGIEPKVFYKAYYQKLYSHLSTACFFDKETFGEDRLVAGMGS
                     LPWPEFVGKTPLSEKVRKDIARLYTEKKDYLPGLSRDEKTARLGKTSYAKFLTDICKA
                     DPAVLPFFQSYTHDLFAVGIDAVSALGCYRNPDDYGAWQFPGFDGMNLGQIEKEEPYI
                     FHFPDGNGSVARLLVRALLPECMPGHTMEDVVTARAQYSKLDQASSNVRIRLNATAVR
                     ARHSGPPESAKEVEVAYVREDKLQTVKAKACILACYNGIIPYLCPELPEKQQEALHYG
                     VKEPFIYTHVAIRNWTSFQKLGIRQIVSPGSYHCFTMLDFPVSLGAYQFPSKPEEPMV
                     LFMLRTPCKPGVPRREQYRMGRYELIGTEFSTFERNIRDQLNRMLASGGFDSARDIAA
                     ITVNRWGHGYAYEYESLSDPHWAPEERPCVVGRKKFGRISIANSDAGASAYTNAAIDQ
                     AYRAVQEQLKDI"
     gene            complement(2107..3786)
                     /gene="ggt"
                     /locus_tag="VEJ00_13555"
     CDS             complement(2107..3786)
                     /gene="ggt"
                     /locus_tag="VEJ00_13555"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXX02237.1"
                     /translation="MRRLTLLLTTLCVWLNPALAQDRDYGRSMVVTDRGIVATSHVLA
                     SQAGAEILSQGGSAIDAAIAANAVLGVTEPMMNGIGGDLFLIYWDAKSGKLYGLNASG
                     WAPQGLTIDFLKKRGITSMPEQGIESVTVPGAVDGWAKAHQRFGKLPWKSLFAPAVYY
                     AETGYSVPEIIHDFWGIAQRRLSQTPEAQRLFLPGGKPPAVGEKFSNPDLGKTLRLIA
                     EKGPREFYEGEIARAILKTSDSLGGTMQASDLAEFSSEWVEPISIDYRGWKVYELPPN
                     GQGMAALEMLNIMATSNADSQGPQGAVELHKKIEAMKLAYTDLYRYNGDPRFAKVPVR
                     GLLSKEYAEQRAALIDPQKANCAAASGTPAKSDTTYLAVVDKDGNIASLIQSVYDYFG
                     SGVAVTGMGFLLQDRGGLFVLDPNHPDALAPRKRPFHTIIPAFMERGDEHIGFGIMGG
                     SNQPLAHAQFVSNVADYGWNIQAALSAPRFTIRDTGESIKCTIVMESRVAPEVQAQLR
                     QKGHDLVMRKEYSALMGRGQAVLHNSATGMNFGASDPRADGAAEPELIPIR"
     gene            4082..4702
                     /locus_tag="VEJ00_13560"
     CDS             4082..4702
                     /locus_tag="VEJ00_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HXX02238.1"
                     /translation="MANKQTNPKSAERINAQPRLHQRAREIQAYGTVNHALPLDLEEP
                     VRLEMTEQLNQLLADTMTLRDLYKKSHWQVAGPTFYQLHLLYDKHYDEQVELVDALAE
                     RIQLLGGVSLAMAADVAETTQIERPPRGREEVPVQLSRLLDAHQTIIREVRKLARLAS
                     ELGDDGTNDLVVSEVLRTNELQVWFLSEHLVNVPLVEAKEPSVNVA"
     gene            complement(4845..6062)
                     /gene="rocD"
                     /locus_tag="VEJ00_13565"
     CDS             complement(4845..6062)
                     /gene="rocD"
                     /locus_tag="VEJ00_13565"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HXX02239.1"
                     /translation="MPVTEISHVQELIGLEEQFGAHNYHPLDVVIKRAEGVWVTDIEG
                     RHYLDCLAAYSAVNQGHCHPRILQTLVEQAHKVTLTSRAFRNDQLPLLCQELHKLTGF
                     DMALPMNSGAEAVESAIKAARKWGYKVKGIPEGQAEIIVCANNFHGRTVTIVSFSTDQ
                     QYRDGFGPFTPGFKVIPYGDAAALREAVTPNTCAFLVEPIQGEAGIIIPPAGYLKEAA
                     AICREHQVLLMCDEIQSGLGRTGKLFAYMHEEIRPDVLIIGKALSGGFYPVSAVLASK
                     EVLGVFHPGDHGSTFGGNPLACAVGRMALRVLDEENLVERSAELGAYFLHRLQTLSSP
                     DLKEVRGKGLWIGIELHSAARPYCEQLKDEGILCKETHDRVLRIAPPLTITREEIDWA
                     FERIRRVIEKRRA"
     gene            complement(6086..7072)
                     /gene="pyrB"
                     /locus_tag="VEJ00_13570"
     CDS             complement(6086..7072)
                     /gene="pyrB"
                     /locus_tag="VEJ00_13570"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HXX02240.1"
                     /translation="MPDATLARPGVTDSFELEAESAGQKLRHLIESQQLTVPLLMELF
                     QRSRGMERVVARGGTLDYQNKILATLFYRPSTRTRLCFEAAMQRLGGRVLSTEHAGTF
                     SSEVEAEQIEDTIRIIGSYADVIVVRHPEAGGAKRAAQVSPVPVINAGDGGGGQHPTQ
                     ALLDLYTIYRERPLIGLSVAIVGELDKGRTARSLVYLLAKFDRVKIFLVSPPELRMKS
                     DILDYLNEHGVRYEVESDIDRVIREVDVIYQTRIRPERVRDERELHRYAIDSSVLRKM
                     KQDTLILHPLPRSVELDKTVDDDPRALYFKQAANGLYVRMALLTMLLERNDS"
     gene            complement(7076..8083)
                     /gene="arcC"
                     /locus_tag="VEJ00_13575"
     CDS             complement(7076..8083)
                     /gene="arcC"
                     /locus_tag="VEJ00_13575"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HXX02241.1"
                     /translation="MRSDSQSVLLAVGGNSLIRAGEKGTVAEQLANARRTAAAIIGLI
                     RGGYHLVVTHGNGPQVGAELLRSERAADQVPGQTLDVCGAASQGEIGYMLAQSLDDEL
                     AAARLHVPVVSLVTQCVVSAHDEAMQHPTKPIGPFYSRTQAEERQRQLGWQIVEDAAR
                     GYRRVVPSPEPVEIVELNVIRSLMNDGVLVVACGGGGIPVIRQNGTLHGVEAVIDKDR
                     ASALLASQLGVDVFAISTDTDYVYLDYKKPTQRPLTNVGASELARHLDQGQFPPGNMG
                     PKVESALRFLRDGGREVVITSYEHLCEAIAGGAGTHIVPDSSVATAKLKAQLEAPVGG
                     R"
     gene            complement(8097..9068)
                     /gene="argF"
                     /locus_tag="VEJ00_13580"
     CDS             complement(8097..9068)
                     /gene="argF"
                     /locus_tag="VEJ00_13580"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXX02242.1"
                     /translation="MGRHNFLSIKDFSPEEIRYLLDLAREMKTHPSAHTSVLKGKTLA
                     MIFEKPSLRTRVSFDVGIQQLGGFSLYLSNAEINLGKRESVYDVAKNLERLVQAIMIR
                     TYAHDIVERMAEYASIPIINGLTDYSHPCQAMADYLTMQEIKGNVAGLKIAFIGDGNN
                     VCHSLMFAGAQLGANVWVATPPGYEPKPEAVNWARQRGSETGGTCMLTHDPVEAANDA
                     DVVYTDVWASMGQEAEQDLRRKIFLPYQVNSALIGRAKYDAIFMHCLPAHRGDEVTDD
                     VIDSAHSVVYQQAENRLHTQKAILLELMQYREVIEPAEVAAHELTHV"
     gene            9545..10621
                     /locus_tag="VEJ00_13585"
     CDS             9545..10621
                     /locus_tag="VEJ00_13585"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: protein motif:HMM:NF006048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HXX02243.1"
                     /translation="MKRYRIAVIEGDGIGREVVPEGIRVLEAAARRFDFSFSWQHFAW
                     SCETYLKTGRMMPEDGLEQIRPFDAIYLGAIGYPGVPDHVSLWGLLIPIRRGFNQYVN
                     LRPVRLLQGITSPLRGRKPGDIDFVIVRENSEGEYSEVGGRVNRNTESDMAIQEVIFT
                     RRACDRIITYAFELARTRRKHVTSATKSNGFVYSMPFWDERFTAISKQYPDVKADQFH
                     IDILTAHFVAHPDWFDVVVGSNLFGDILSDLGAAVVGSMGLAPSANLNPEREYPSMFE
                     PVHGSAPDIAGKTIANPIAQIWSGAMMLEHLGEQQAARAVESSIFNVLANGSVRTRDI
                     GGSAGTQGMGEAIAAEVSAALIAR"
     gene            complement(10697..>11740)
                     /locus_tag="VEJ00_13590"
     CDS             complement(10697..>11740)
                     /locus_tag="VEJ00_13590"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXX02244.1"
                     /translation="GRFMGKTDLGDGYTRWDWFVHYPINNYDVSVNIGNYQHFSDRLG
                     DLDMDFYVLPEDMEKARKQFSQAKGMIEAFEHYFGEYPFKKDGYKLVEVPYSGMEHQS
                     AVTYGNHFANGYLERDWTGVGISPRFDFIIIHESGHEWFGNSITAADPSDMWIHEGWT
                     TYLESLYVEYMYGKADAIKYLNGYMSKVKNERPIITLRGVNGEPPPDQYFKGVLFINT
                     LRSIVDDDKRWWKLLHDLYQHFKYQNIMTDDMEQFFNQQTGMNLTPIFDQYLRHPSIP
                     TLELKFDNAGGTVSYRWKTDEPGFQMPVRVGKKDSWQIVHATPDWQVMRTALKKDDFE
                     VATDLYYVNVARQ"
BASE COUNT         2410 a         3458 c         3368 g         2504 t
ORIGIN      
        1 cgtcctgcgc gcccggcggg gagcccgacg tcgcctgcgc gctcagcagc gtgggaagcc
       61 acgaactggc catcgccccg ccggcgctga ccgcgattcc gtttagaaaa tcccggcgcg
      121 taatgctccg gtgcattccc agttcgtgat cccgcgacgc tctattcttc atagtgttat
      181 atatccttca attgctcctg gacagcgcga tacgcctggt cgatcgccgc attcgtgtag
      241 gcactggcgc ccgcgtcgga atttgcaatc gagattcggc cgaacttctt tcggcccacc
      301 acacagggac gctcctccgg cgcccagtgc ggatccgaaa gtgactcgta ctcgtaggcg
      361 tatccgtggc cccagcggtt gacggtgatg gctgcgatgt cgcgggcaga gtcgaatccg
      421 ccggatgcga gcatgcggtt cagctgatcc cgaatattcc gttcgaacgt agaaaattcc
      481 gtaccaatca actcgtatct tcccatccgg tactgctctc gccgcggcac gccaggcttg
      541 cagggcgtgc gcagcatgaa cagcaccatc ggttcctcag gcttgctcgg gaattgatag
      601 gcaccaaggc tgaccggaaa gtcgagcatc gtgaaacagt gatagctgcc gggtgacaca
      661 atctgtctga tgccgagctt ctgaaacgag gtccagttgc ggatcgccac atgcgtgtag
      721 atgaacggct ctttgacacc gtagtgcagc gcctcctgct gcttttccgg cagctctgga
      781 caaagatagg gaatgatgcc gttgtaacag gccagaatgc aggccttcgc cttcacggtc
      841 tgcagtttgt cctcgcgcac ataggctact tcgacttctt tcgccgattc tggcggtccg
      901 gaatgacggg cgcgaaccgc ggttgcgttc aatcggatgc gtacgtttga cgatgcctgg
      961 tcgagcttgc tgtactgggc gcgggctgtg accacgtctt ccatggtgtg tccaggcatg
     1021 cattcgggga gcaaagcgcg gacgagcagc cgcgccaccg agccgttgcc gtccggaaaa
     1081 tgaaaaatgt aaggctcttc cttttcgatc tggccaaggt tcatgccgtc gaagccgggg
     1141 aactgccatg ctccgtagtc atccgggttc cggtaacacc ccagcgcgga aactgcatcg
     1201 attcctaccg caaacagatc gtgcgtatag ctctggaaga aaggaagcac cgcaggatcg
     1261 gccttgcaga tgtcggtcag gaattttgcg tagctggtct tacccaaccg tgccgtcttt
     1321 tcatcacgcg acagacccgg caaatagtcc ttcttctctg tatagagccg cgcaatgtct
     1381 ttgcggacct tttcggaaag cggcgtcttg cccacaaact ccggccaagg cagcgatccc
     1441 attcccgcta ccaggcgatc ttcgccgaac gtctctttat cgaagaaaca cgccgtactc
     1501 aaatgcgaat agagtttctg atagtaagcc ttgtagaaaa ccttcggctc gatgcccaaa
     1561 tctttcagaa cgctcttgga cacctcactg tattcggaag ggctttcaat cgactgagtt
     1621 ccgccattgc tcaaaagcag gcggccgtcc acctggaatt cgtttcgccg gcaatgtccg
     1681 ccgaaatcat cgtgattgtc gaggatgaga attcgcgcat ctttgccggc gtgcttgcga
     1741 tagaaatagg ccgcggccag cccgctgatc cctcctccca cgactaccac gtcgtaagtc
     1801 tcatgtgtat cctctggtgt tcctgccgtt tcccaaaagg ttccgtcccg cagggaatga
     1861 gcaacgccaa acgaacccgg ctggctgcct ctcatcccgg tcaatcctgg cggataggat
     1921 ggggcggggt gcccatccga ctggcctgtg ctttgagcgg ctcttgcgaa atcggctata
     1981 ccggcagatc ccaaagcgcc tacagcgaca gccatcccat tgaggaaatc gcggcgcgtg
     2041 atgcgcgtgg ccatgcccag ttctcgatcg cgcttttcgt cgtccataga ttttctagag
     2101 cgctcgctaa cggatcggta ttagttccgg ctccgccgct ccgtcggctc gcgggtcaga
     2161 agcgccgaag ttcatgcccg tagcggagtt gtgcaacacg gcctggcctc gtcccatcag
     2221 cgccgaatat tctttgcgca tcacgaggtc atggcctttc tgacgcaact gcgcctgcac
     2281 ctccggtgct acccgcgatt ccatcacaat ggtgcacttg atcgattctc cggtatcgcg
     2341 gatcgtgaaa cgcggagccg acaaggccgc ctgaatgttc cagccgtagt ccgcaacgtt
     2401 ggagacaaac tgcgcatgcg ccagcggctg attcgagccg cccatgattc caaaaccaat
     2461 gtgctcgtca ccgcgttcca tgaacgccgg aataatggtg tggaagggac gcttgcgggg
     2521 cgccagggca tcgggatggt taggatcgag cacaaacagc ccgccccgat cctgcagcag
     2581 aaaacccatc cctgtcaccg cgaccccgga accgaagtag tcgtacacgc tctggatcag
     2641 cgacgcgatg ttgccgtctt tgtcgaccac ggctagatac gtcgtgtcgc tcttcgccgg
     2701 cgtgccgctc gccgccgcgc agttggcctt ttgcggatcg atgagcgcgg cgcgctgctc
     2761 ggcatactcc ttcgagagca gcccacgcac cggcaccttg gcgaagcgcg gatcgccgtt
     2821 atagcggtac aggtcggtat aggcgagctt catcgcctcg attttcttgt gcagctcaac
     2881 cgcgccttgc ggaccctgcg aatccgcgtt cgacgtggcc atgatgttca gcatctcgag
     2941 cgcggccatc ccttgaccgt tgggtggaag ttcgtagacc ttccagccgc ggtagtcgat
     3001 cgagatcggt tccacccact ccgatgaaaa ctctgccagg tcactggcct gcatcgtgcc
     3061 gcctagagaa tccgaggttt tgaggatcgc cctcgcaatt tcgccctcgt aaaactctct
     3121 cggacctttc tcagcgatca accgcaaggt cttccctaga tcgggattcg aaaatttctc
     3181 ccccacggcc ggcggcttcc ccccaggcaa gaagagacgc tgcgcctccg gcgtctgact
     3241 caatctccgc tgcgcgatgc cccagaagtc atggataatt tcaggcacgg aatagccggt
     3301 ttcggcgtag tagacagccg gggcaaacaa ggacttccaa ggcagcttcc caaaacgttg
     3361 atgggcctta gcccatccat cgactgcacc cggcactgtg acactctcga tcccttgctc
     3421 aggcatggag gtaatgccac gttttttgag gaagtcgatg gtcaggccct gcggcgccca
     3481 tccactcgcg tttagaccat agagcttgcc gctcttcgcg tcccaataga tcagaaacag
     3541 atcgccgccg atgccgttca tcatcggctc cgttacgccc agcaccgcat tcgcggcaat
     3601 cgctgcatcg atggcggaac cgccctgtga cagaatttct gcgcccgcct gtgaggccag
     3661 gacgtggctg gtggccacga tcccgcgatc ggtaacgacc atggatcgcc cgtaatcgcg
     3721 atcctgagcc agcgctggat tcaaccaaac acacaatgtc gtaagcaata aggtcaggcg
     3781 gcgcattgag ggtccccggc tttgcggtcg aagagagtgt gtcagctttt cagctgtgca
     3841 gattactaca agtccgtaag gttagaaaaa ctggcgagaa cgtgtcaaga aaggaatgct
     3901 tcaaagaacc aaaacggggt ttggtgtacc atcccacggt gagagccgtc cgagccgctg
     3961 cacgcccatc agcgtgaccg cctttttgat cggattcgct gggactggga tctgacgctt
     4021 taaagtctga tgaacccatt gtcgtccgaa ctccatctat gtcttgaaag gaggctaacc
     4081 tgtggcaaac aagcagacca acccaaagag tgcagaacga atcaacgcac aacctcgcct
     4141 acatcagcgt gcgcgggaga ttcaggccta cggaaccgtc aaccatgcac tgccccttga
     4201 tctcgaggag ccagtgcgcc tggaaatgac cgagcagttg aaccagctgc ttgcggatac
     4261 gatgaccctg cgtgacctgt acaagaaatc ccattggcaa gttgcgggtc caacgtttta
     4321 tcagctgcat ttgttgtacg acaagcacta cgacgagcaa gtcgaactgg ttgatgccct
     4381 agcggagcga attcagcttc tcggcggagt cagcctggcg atggcagcgg atgtcgctga
     4441 gacaacccag atcgaacgcc caccccgcgg gcgcgaagaa gtcccggttc agctgtcgcg
     4501 attgctcgac gctcaccaaa ccatcattcg ggaagtgcgg aagttggctc gcctggcatc
     4561 ggaattgggc gatgatggca cgaatgacct tgtcgtgagc gaggtgcttc gcacgaatga
     4621 acttcaagtg tggttcttga gcgaacacct ggtgaacgtg ccgttggtgg aggcgaagga
     4681 accgtcggta aatgtggcgt aggaattcca caataaccgg aaaatggatc ttctgaaact
     4741 gtaacggggg gaacttgcgc agggacgaac cccaggggag acgtatgtcc tggagcagaa
     4801 ggatctcgca acgggaatgt ggtgagactc tcgagcccgg tccgttacgc ccgtcttttt
     4861 tcgatcacac ggcggatgcg ctcgaaggcc cagtcaattt cttcccgcgt gatagtcagc
     4921 ggaggggcga ttcgaagcac cctgtcgtgg gtctccttgc agaggatacc ttcgtctttc
     4981 agttgctcgc aataaggacg cgcagcggaa tgcagttcga tgccgatcca gagtcccttg
     5041 ccgcgcacct ctttcagatc gggactgctc agggtctgca gacggtgcag gaagtaggcg
     5101 cctaactcgg ccgagcgttc caccaggttc tcttcgtcca ggacgcgcag agccattcgc
     5161 cccaccgcac aggccagagg atttccgccg aatgtgctgc catggtcgcc gggatggaaa
     5221 acccccagaa cttctttcga cgccagcacg gccgatacgg gatagaaccc tccggacagc
     5281 gctttgccga tgatcagcac atcaggcctg atttcttcgt gcatgtaagc gaagagcttg
     5341 ccggttctgc caaggccaga ttgaatctcg tcacacatga gcaatacctg gtgctcgcgg
     5401 cagatcgctg cggcttcttt caggtagccg gccgggggaa taatgatgcc ggcctcgccc
     5461 tgaatcggtt cgaccagaaa ggcacaggta ttcggagtaa cagcctcccg caatgcggca
     5521 gcgtcgccat aaggaatgac tttgaatccg ggagtgaacg gtccgaaccc atcacgatac
     5581 tgctggtcgg tcgagaaact gacaatggtc acggtgcggc cgtggaaatt gttggcgcac
     5641 acgatgattt ccgcctgccc ttccggaata cccttgacct tgtatcccca cttgcgcgcc
     5701 gcctttatcg cggattcgac agcctctgcg ccagagttca tgggaagcgc catatcgaac
     5761 ccagtgagtt tgtgcagttc ctggcagagc aacggcaact ggtcgttacg gaatgcgcgt
     5821 gaagtgagcg tcaccttgtg ggcctgctcg accaacgtct gtaagatccg gggatggcaa
     5881 tggccctggt tgaccgcgga ataagcggcc agacaatcga ggtagtggcg gccctcaatg
     5941 tcggtcaccc agacaccctc ggcgcgcttg atcacgacgt cgaggggatg atagttgtgg
     6001 gcgccaaact gctcttcgag cccgatgagt tcctgaacat gactgatttc cgtgaccggc
     6061 attgctgtct cctccactgc ggaagttagg aatcgtttct ttccaagagc atcgtcagca
     6121 gcgccatgcg cacatacaaa ccgtttgccg cctgcttgaa ataaagcgcc cgcggatcgt
     6181 cgtccacggt cttgtcgagt tctacggacc gtggaagcgg gtgaagaatc agggtatcct
     6241 gcttcatctt tcgaagaacg gaggagtcga tggcgtaacg gtgcaactcg cgttcgtcgc
     6301 gcacacgctc gggacgaatg cgggtctggt agattacatc cacttcgcgg atgacacggt
     6361 caatgtcgga ctcgacctcg tagcgcacgc cgtgttcatt gagatagtcg agaatgtcgc
     6421 tcttcatgcg caattcaggc gggctgacca gaaaaatctt gactcgatcg aacttcgcca
     6481 ggaggtacac gagagagcgt gcggtccggc ctttgtccag ttctccgacg atggcaacgc
     6541 tcaaaccgat cagcggccgc tcgcgataaa tcgtgtacag atcgagcaga gcttgcgtgg
     6601 gatgctggcc acctcctccg tctcctgcat tgatcacggg aacgggtgag acctgggccg
     6661 cgcgcttcgc ccctcccgcc tcgggatgac gcacgacgat cacatcggca tagctgccaa
     6721 taatacgaat tgtgtcctcg atctgttcgg cttcgacttc agaggaaaac gtccccgcgt
     6781 gttcggtgga gagcacccgg ccgcccagcc tctgcatggc tgcttcaaag cagagacggg
     6841 tgcgggtgga ggggcggtag aacagggtcg ccaggatctt gttctgatag tcgagggtgc
     6901 ctccgcgcgc cacgacccgc tccatgccgc gggagcgctg aaacaactcc atgagcaagg
     6961 gcacggtcag ctgctgcgat tcgatcaagt gtctcagctt ctggccagca gattccgctt
     7021 cgagctcaaa cgagtccgtg acgccgggcc ttgccaacgt tgcgtcaggc atatttcacc
     7081 gccctcccac tggcgcttcg agttgcgctt tgagtttggc cgtggccaca ctcgagtcgg
     7141 gaacaatgtg cgtaccggcc ccgccggcaa tcgcctcaca gaggtgttca tacgaggtaa
     7201 tgaccacttc cctgccgcca tccctgagga agcgcaatgc agattcaact ttcggtccca
     7261 tgtttccagg gggaaactgt ccctggtcga ggtggcgtgc aagttcggac gcaccgacat
     7321 tggtcagcgg acgctgcgtc ggcttcttgt agtcgagata cacgtagtcc gtatcggtcg
     7381 agatggcgaa gacgtccacg ccgagctgtg aagccagcaa ggctgacgcc cggtccttat
     7441 caatgacggc ttccacaccg tggagagttc cgttttgccg gatcaccgga atgccgccgc
     7501 cgccgcaggc caccaccaga actccatcgt tcatcagcga acgaatgacg ttgagttcaa
     7561 cgatctccac cggttcaggc gaaggcacga cccggcgata accacgtgcc gcgtcttcca
     7621 cgatctgcca acccagctga cgttggcgct cttctgcctg cgtccgcgaa tagaaaggac
     7681 cgatgggctt ggtgggatgt tgcatggctt catcgtgggc tgacacgacg cactgcgtca
     7741 ccaagctgac aacaggaaca tgcaggcgtg ctgcggccag ttcatcgtcc agcgactgcg
     7801 cgagcatgta cccgatttcg ccctggctgg cggcgccgca gacgtcgagg gtttgaccgg
     7861 gtacctgatc ggccgcgcgt tccgaacgca gcagctctgc gcccacctgc gggccgttgc
     7921 catgggtcac gaccaagtga tagccaccgc gaatgagccc gatgatcgcc gccgcggtcc
     7981 gccgggcatt ggccagttgc tcggccaccg ttcctttttc tccggcccgg atgagggagt
     8041 ttccccccac cgcgagtaga acggattggg aatcggatct cactgctgtg ctccgttcag
     8101 acgtgcgtca gttcgtgtgc cgcaacttct gccggttcga ttacttcccg gtattgcatc
     8161 agctccaaca ggatcgcttt ctgcgtgtgc aagcggttct ccgcctgctg atagaccacc
     8221 gagtgcgcgg aatcaatcac atcatcggtg acttcatcgc cccggtgggc ggggaggcaa
     8281 tgcatgaaaa tcgcatcgta ttttgcgcgg ccgatcaggg ccgaattcac ctggtagggc
     8341 aagaatatct ttcggcgcag atcctgctcc gcttcctggc ccatgctcgc ccagacgtcg
     8401 gtgtaaacga catcggcatc gtttgcggct tcgaccggat cgtgcgtcag catgcacgtc
     8461 ccaccggttt ccgagccgcg ctggcgcgcc caattcacag cctccggctt cggctcgtat
     8521 cccggcggag tggccaccca tacgttggct cccagttgtg ccccggcaaa catcagcgag
     8581 tgacacacgt tgttgccgtc gccgataaac gcaatcttca gcccggctac gttgcccttt
     8641 atttcctgca tggtcagata gtcggccatg gcctggcagg gatggctgta gtcggtcagg
     8701 ccgttgatga tcggaatgct ggcatactcc gccatccgct ccacgatgtc gtgagcatag
     8761 gtgcgaatca tgatggcctg cacaagccgc tccaggttct tcgccacgtc gtacaccgac
     8821 tcgcgcttgc ccagattgat ttcggcgttc gagagataaa gggaaaaacc gcccagctgc
     8881 tggattccga catcaaacga gacgcgcgtg cgcagcgatg gcttctcaaa gatcatggcc
     8941 agcgtcttcc ccttcaatac cgacgtgtga gcggaggggt gcgtcttcat ctcgcgggcc
     9001 agatcgagca ggtaccggat ttcttcaggt gaaaagtcct tgatggaaag aaagttatga
     9061 cgacccatag ggagccccag ttctccgggc ggcggctgcc ttgcgcccgg tttggaactc
     9121 ggcaggactt ggcaccccct ctgctatccg attgaggaca caagggaaag ccggccttag
     9181 ccgaggatgt tggcaagcga gaatgtgtgg tgagggcaag ccgaatacaa catcagggag
     9241 cacccgctgg cattgctgcc gttggctgta acttgcagga aacacggcaa caccccctaa
     9301 ttatcatgtg gtttcagggg cgcgatggcc gtgaaaggcg tcaccgcatc gagtgatggg
     9361 ggtcacgcgc gttgcagcgt catcacgagg cccggtagtg ggctcttttg aaaacagccc
     9421 actacccgag gccccctccg gaagccgaaa ggggtggcta gggtaagatg gcaccggtcc
     9481 cgagacgttt gcatgctggc cgccttgatg aacaaagtga ctgcgaaggt ccatgagagg
     9541 aaacttgaaa cgctaccgga tcgcggtgat tgaaggagac ggcatcggac gcgaagtcgt
     9601 ccctgagggc attcgtgtgt tggaggcggc ggctcgccgg ttcgactttt ctttttcctg
     9661 gcagcatttc gcttggagct gtgaaactta ccttaagacc ggccgcatga tgcccgaaga
     9721 tggattggag caaattcgtc ccttcgacgc aatctacctg ggtgcgatcg gctatccggg
     9781 agtacccgac cacgtttctc tatggggatt gctgattcca atccggcgcg gtttcaacca
     9841 gtacgtgaat ctgcggccag tccgtctgtt gcaggggatc accagccccc tcagaggacg
     9901 caagccgggc gacatcgact tcgtgattgt gcgggaaaac agcgaaggcg aatactctga
     9961 agtgggcggg cgcgttaatc gcaacaccga atccgacatg gcgatccagg aagtgatctt
    10021 cacgcgacgt gcctgcgacc gcataattac ctacgctttc gaactggcgc gtacccgcag
    10081 aaagcatgtc acctcggcga ccaagtcgaa tggatttgtg tactcgatgc ccttctggga
    10141 tgaacgtttc accgctattt ctaagcagta cccggatgtg aaagccgacc agtttcacat
    10201 cgacattctc accgctcact tcgtggcgca tcccgattgg tttgacgtgg tggttggctc
    10261 aaacctcttc ggcgacatcc tttcggatct cggcgctgcg gttgtcggct ccatggggct
    10321 ggcgccctcc gcgaacttga atccggaacg ggaatacccc tcaatgttcg agcccgtaca
    10381 cggctcggcg ccggacatcg caggaaaaac gatcgcgaac ccgatagcac aaatctggtc
    10441 gggcgcaatg atgctggagc atctgggcga acaacaagcc gcgcgcgccg tggagagctc
    10501 gattttcaat gtgctggcca acggaagcgt gcgaacacgg gatattggcg gcagcgccgg
    10561 cacccagggc atgggtgagg ccatcgccgc ggaagtctcc gctgccctca tcgcccggta
    10621 gcttgctgca gcagttccct tttcttccct gttgagatca acgacatcgg tacgaacgca
    10681 gcacagcgcg gaggccctac tgtcttgcga cgttcacata gtaaagatcg gtggccactt
    10741 caaagtcatc tttcttcaaa gcagttcgca ttacctgcca gtccggggtg gcatggacaa
    10801 tctgccaact atctttcttc ccgacacgta ccggcatctg aaaccctggt tcatccgttt
    10861 tccagcggta tgacacggtg ccgccagcgt tgtcgaattt caattccaag gtagggatgg
    10921 acgggtggcg taggtactga tcgaaaatcg gcgtcagatt cattccggtc tgctggttga
    10981 agaactgctc catgtcatcc gtcataatgt tttgatactt gaagtgctgg tagagatcgt
    11041 ggagcagttt ccaccagcgc ttatcatcat caacaatgct ccgcagcgtg ttgatgaaga
    11101 gtacgccctt gaagtactgg tccggcggcg gctccccgtt tacgccgcgc aaggtgatga
    11161 taggccgttc atttttgacc ttggacatgt acccattcaa atacttgatg gcgtctgcct
    11221 tgccatacat gtattcgacg tagaggctct ccaggtaggt cgtccagccc tcatgaatcc
    11281 acatatctga ggggtcggcg gcggtgatgc tgttgccgaa ccattcatgc ccgctctcat
    11341 gaatgatgat gaagtcgaac cgcggactga tgccgacgcc cgtccagtcg cgctcgagat
    11401 agccattggc aaagtgattt ccgtaagtaa cggcgctctg atgttccatt ccggagtagg
    11461 gcacctcgac cagtttgtag ccatcttttt tgaagggata ctcgccaaag tagtgctcaa
    11521 atgcctcgat catccccttg gcctgggaga attgcttcct ggctttctcc atgtcttcag
    11581 gaagcacgta gaagtccatg tcgaggtcgc ccagccgatc ggagaagtgc tgatagttgc
    11641 cgatgttgac ggagacatcg taattgttga tggggtagtg aacgaaccaa tcccagcgag
    11701 tgtagccgtc gccgaggtcg gtcttgccca tgaaccttcc
//
LOCUS       DASPUL010000583         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1610836, whole genome shotgun sequence.
ACCESSION   DASPUL010000583 DASPUL010000000
VERSION     DASPUL010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610836"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..744)
                     /locus_tag="VEJ00_13595"
     CDS             complement(109..744)
                     /locus_tag="VEJ00_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02245.1"
                     /translation="MQLGLDNKRKTTWAAVLGTLAVLALAYQFIPLLTGPSTSPTSSA
                     EAAGPQAIRVSTHGAAGAKNLKKARAETLDPTLRLDLLAASEQTQYQGTGRNIFVPQA
                     EEVKIPTVVARPNTDGVAAAAAPPPIYVPPQPPAAPPIPLKFYGFANQPGEPRKVFLE
                     QGEDVWVAGEGEIVNRRYKVVRITPNSVSIQDVVNSGPPQNIPLTSGQGQG"
     gene            complement(744..1283)
                     /locus_tag="VEJ00_13600"
     CDS             complement(744..1283)
                     /locus_tag="VEJ00_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02246.1"
                     /translation="MPDLRQTRKNLKIALAAMLGVDLVMAVVYLSPVVGSADSRRQEL
                     NRLQAELNTKTRQVAPLKDVDKKVVLAQGQIADFYKKRFVSQNSEILTEFGKLASANG
                     VTIEQGKYKVTGDGPGGLEVVTMEADLGGNYTALAKFINALERDQTFFIINNITLGGE
                     QQGPVKLGMKLETYLKAGS"
     gene            complement(1283..1927)
                     /locus_tag="VEJ00_13605"
     CDS             complement(1283..1927)
                     /locus_tag="VEJ00_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02247.1"
                     /translation="MRLDINLASQPYEDARQFWMRWGTAVALVGLLTFVLLTMTVRGW
                     VFASRDRSAMAQKRALIAQRDERRAEAEKILNMPQNRITRDESQFLNQLIERKSFSWT
                     LVLESLEKVMPPRVHLMSISPGLDEDNQLLLKMSVAGDSRERALELARRMEESKRFAG
                     TSIVSEHYVQPGSGNTDTEQFDIVAFYVPEPVVTSGPDAKPEVRTVSETLPSQH"
     gene            complement(2005..2901)
                     /locus_tag="VEJ00_13610"
     CDS             complement(2005..2901)
                     /locus_tag="VEJ00_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02248.1"
                     /translation="MSTSANTAKPKLACEIAADRVLAGRLNDHGGGLEVCAARELAPG
                     SVVPDLAEGNLRQRDAVRAGIEATLGSVAGRSRDVIAVVPDAAVRVMLVEFDTLPSDH
                     EEALGVVRFRLKKSLPFDVDKAKVSYQAQKFSDGVRVVAAVALASVIEGYEAVFRDAG
                     FNPGVVLPSMLAALGAADGRRPTLVVKVDAHTTSIAILNEDQLQLFRTLENARGVTIT
                     GEQLAEEVYPSVVFFQDTYHLNIERIFVAGLPESSGTAAALRAQTGAEVQELVGSSQL
                     GASTGGSVPRWRMAGVVGALVS"
BASE COUNT          559 a         1179 c          851 g          642 t
ORIGIN      
        1 ccggtttcga gttgatgtct gccgatgcat ctgaagccga ttcatccgcg gcccggccgg
       61 cttgccattc cgaggagcgc agcgacgagg aacccctgtc cagcgccctt acccttgccc
      121 ttgccccgag gtcagcggaa tattctgcgg cgggccgctg ttcaccacgt cctgaatgga
      181 aacggaattg ggcgtgatgc gaacgacctt gtagcgccgg ttcacgatct cgccttcgcc
      241 tgccacccaa acgtcttcgc cctgttccag gaacactttt cgcggttctc ccggctggtt
      301 ggcgaatccg taaaatttca ggggaatcgg cggcgccgct gggggctgag gtgggacgta
      361 aattggaggc ggggccgcag ccgctgccac cccgtccgta ttgggccggg ctaccaccgt
      421 gggaatcttt acttcctccg cctgcggcac aaaaatattc cttcccgtcc cctgatactg
      481 tgtctgttca ctcgccgcca gcagatccag ccgcagcgtc ggatccagcg tctccgcccg
      541 cgctttcttc agattcttcg ccccggcggc gccatgcgtg ctcacccgga ttgcttgtgg
      601 gcctgccgcc tccgcactcg aagtcggaga cgtcgatggc cccgtgagca atgggatgaa
      661 ctgataggcc agcgccagca cggccagtgt ccccagcacg gccgcccacg tcgtcttccg
      721 cttgttgtcc aatcccagtt gcactacgat cccgccttca aataggtttc cagcttcatg
      781 cccagtttca ccgggccctg ctgctcaccg ccgagcgtga tgttgttgat gatgaaaaac
      841 gtctgatccc gctccagcgc attgatgaac ttggccagcg ccgtgtaatt gcccccgagg
      901 tccgcctcca ttgtcacgac ctcgagcccg cccggcccgt caccggtcac tttatatttt
      961 ccttgctcga tagtgactcc attcgccgat gccagcttgc cgaactcggt cagaatctcg
     1021 gaattctgcg acacgaaccg cttcttgtag aagtcggcga tttgtccctg cgccagcacg
     1081 accttcttgt ccacatcttt caacggcgcc acctgccgtg tcttcgtgtt caactccgcc
     1141 tgcaggcggt tcagctcctg gcgccgcgaa tccgccgaac cgaccaccgg cgacagatac
     1201 acgaccgcca tcaccagatc cacgcccagc attgctgcca gcgcgatctt cagattcttc
     1261 cgggtttgtc tcaggtcggg catcagtgct ggctcggcaa agtctcgctg accgtcctaa
     1321 cttcgggttt ggcgtcgggg ccgctcgtaa ctaccggctc gggcacgtaa aacgccacga
     1381 tgtcaaactg ctctgtgtcc gtgttcccgc tgcccggctg aacatagtgc tcggaaacga
     1441 tgctcgtccc cgcaaagcgc ttcgactctt ccatgcgccg cgccagttcg agcgcccgct
     1501 cccgcgagtc tcccgccacc gacatcttga gcaaaagctg attgtcctcg tccagtcccg
     1561 ggctgatcga catcagatgc acccgcggcg gcatcacctt ctccaggctt tccagcacca
     1621 gcgtccagga aaacgacttg cgctcgatca gctggttcag gaactgcgac tcatcgcgcg
     1681 tgatgcggtt ttgcggcatg ttgaggatct tttccgcctc cgcccgccgc tcgtcgcgct
     1741 gcgcaatcag cgcgcgtttc tgcgccatcg ccgagcgatc ccggctggca aacacccacc
     1801 cccggaccgt catcgtcagc agtacaaacg taagcaatcc caccagagcc acagcggtgc
     1861 cccagcgcat ccagaactgc cgcgcatctt cgtacggctg actcgcgaga ttgatatcca
     1921 aacgcataac tcagggatca gggatcagag gtcagggatc aggaggtctt gctggcctct
     1981 gatccctggc ccctggcccc tgccttaaga aaccaacgct cccaccaccc ccgccatcct
     2041 ccaccgcggt accgaaccgc ccgtgctcgc gcctaactgc gacgagccca ccagttcctg
     2101 cacctccgct ccggtctgcg cccgcagcgc cgccgccgtt ccgctcgatt ccggcagtcc
     2161 ggccacaaag atccgctcga tattcaggtg atacgtgtcc tggaagaaca ccaccgacgg
     2221 ataaacctcc tccgccagtt gctccccggt aatcgtcacc ccgcgcgcgt tttcgagcgt
     2281 gcggaacaac tgcagctgat cctcgttcag gatcgcgatg ctcgtcgtat gcgcgtccac
     2341 cttaaccacc agcgtcggcc gccgcccgtc ggccgcgccc agcgccgcca gcatcgaagg
     2401 cagcaccacc ccgggattga atcccgcgtc gcggaacacc gcctcatagc cttcgatcac
     2461 gctggccagc gccaccgccg ccaccacccg cacgccgtcg ctgaatttct gcgcctgata
     2521 ggagaccttc gccttgtcca cgtcaaaggg cagagatttc ttcagccgga aacgcaccac
     2581 tcccagcgct tcttcgtgat cggagggcag ggtgtcgaac tccaccagca tcacccgcac
     2641 agccgcatct ggaaccaccg cgatcacatc ccgtgatctt cccgccacgc tgcccagggt
     2701 ggcttcaatc ccggcgcgca ccgcgtcccg ctgccgcaga ttgccctccg ccaggtccgg
     2761 aaccacgctg cccggcgcca gttcgcgcgc cgcgcacacc tccagcccgc cgccgtgatc
     2821 gttcagccgc ccggccagga cccggtccgc cgcgatctcg cacgccagct tcggcttggc
     2881 cgtatttgca gaagtactca ttgcccgcgc acgcctctag taaagaacct cttgcttaaa
     2941 tcagaaacct tatcgccgcc aatttcaata gctcaggggg aactcagctc cgatcacgta
     3001 attcgcgttg tcatcctcag cagcgcgcct tttgcgctgc gaaggacccg ggcgcgccgc
     3061 gcgaatcgcc cgcgcttttt gcgggcgaac aagtcgcgcg ttcggcgcgc atcctcatcg
     3121 catcgcctcg atgaacgtga ctttgttgat ctccttgagc gtcgtcatgc ccagccggac
     3181 tttgtccaag gctgactcgc gcaggaaccg catgccttcc tcgcgcgccg c
//
LOCUS       DASPUL010000584         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_790540, whole genome shotgun sequence.
ACCESSION   DASPUL010000584 DASPUL010000000
VERSION     DASPUL010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790540"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VEJ00_13615"
     CDS             complement(<1..343)
                     /locus_tag="VEJ00_13615"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXX02249.1"
                     /translation="MAAAKTGKTGKKNGLTTPDLVLLSLLAERAMHGYQANLELERRQ
                     VRDWAGISRPQVYYSLEKLARLGLVRAAHTGEPSAGPERSVFEITPRGRSALADALER
                     GEWATQRERPPF"
     gene            complement(330..614)
                     /locus_tag="VEJ00_13620"
     CDS             complement(330..614)
                     /locus_tag="VEJ00_13620"
                     /inference="COORDINATES: protein motif:HMM:NF023159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXX02250.1"
                     /translation="MATQERRLQDLISVGPAMVRDFQILGVRSVSALARRNPGKLYEK
                     LCQLTGQAQDICCLDVFRAAVEQARNPRLPAEQCQWWYWSRQRKARDGRS"
     gene            701..1186
                     /locus_tag="VEJ00_13625"
     CDS             701..1186
                     /locus_tag="VEJ00_13625"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXX02251.1"
                     /translation="MRITIFVLALAVLASVLPLAKPRAEEKNKTMNVKRITPVMFAED
                     VEPCVKFWVERMGFQKTAEVPDGNKLAFAMLQKGNIELMYQSYASAGKEGGSIPQLAR
                     KGPTFLYVEVDDLKEAISATQGAPVVVPVHDTFYGAKEISIKDPAGHFVTFAQFGAAQ
                     H"
     gene            complement(1324..>2373)
                     /locus_tag="VEJ00_13630"
     CDS             complement(1324..>2373)
                     /locus_tag="VEJ00_13630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXX02252.1"
                     /translation="PHLRIDGELVPLTDQPRISAEDMLNMAFSMMSARQKQKFKETTE
                     IDMAYGVAGLGRFRVNIFQQRGNVGLVLRVIPTKIRALDELFLPKVVEQICDMPRGLV
                     LVTGVTGSGKSTTLAAMVDRVNSSRAEHIITIEDPIEFLHRDKKGYVNQREVGVDTPS
                     FGAALRASLRQDPDVILVGEMRDLETISTALHAAETGHMVFSTLHTLDAVETINRVIS
                     IFPPPEQKQIRMQLAAVLRAIVSQRLVRRCDAEGRVPAVEVMINTAYIRECVLVPEKT
                     RAIRDAIAAGTSQYGMQTFDQSLWDLFQAGLVNYETALENASNADDFKLRMQGIASTS
                     DISRQSMQSAGFGGR"
BASE COUNT          461 a          705 c          750 g          457 t
ORIGIN      
        1 gaaagggcgg ccgctcgcgt tgcgtggccc actccccgcg ctcgagagcg tcggccaacg
       61 ctgagcgccc gcgaggggtg atctcgaaaa cgctgcgttc cgggcccgct gacggttcgc
      121 cagtgtgagc tgcgcgaacc aggcccagtc tcgccagctt ctcgagcgaa tagtagacct
      181 gcgggcgcga gatgccggcc cagtcccgta cctggcgccg ctccagttcc agattggcct
      241 ggtagccgtg catggcccgc tcggccagaa ggctgagcag aaccaggtcc ggagtggtca
      301 acccgttttt cttacccgtt ttgccggtct tagctgcggc catcgcgcgc ctttcgctgg
      361 cgactccagt accaccactg gcattgctct gcgggcagcc gcggattccg cgcctgctcg
      421 actgcggcgc ggaacacgtc gaggcagcag atgtcctgcg cctggcctgt gagctgacag
      481 agtttttcgt aaagcttccc ggggttgcgg cgggccagcg cagatacgct gcgtactcca
      541 agtatctgga agtcgcgcac catggccggg cctaccgaga tcagatcctg cagacggcgt
      601 tcctgggttg ccatgaaaat cctccttgac acgatgaggt gagtttagta tcgttagcta
      661 gtctaattta gactacatgg gtttgacaag gataaattcc atgcggatca caatctttgt
      721 tctggctctg gccgtgctcg cgtccgtatt gcccctggca aagcccaggg ccgaggagaa
      781 aaacaaaact atgaatgtga agagaattac cccggtgatg ttcgctgaag acgtggaacc
      841 atgcgtgaag ttctgggtgg agcgcatggg tttccagaaa accgccgagg taccggacgg
      901 caacaaactc gccttcgcca tgctgcagaa gggcaacatc gagctgatgt accaaagcta
      961 cgccagcgcc ggcaaggaag gcggctcgat tccgcagttg gcccgcaagg ggcccacgtt
     1021 tctatatgtc gaggtagacg atttgaagga agcgatctcc gcgacccagg gagcgccggt
     1081 ggtggtgccg gtgcacgata ctttctatgg agcgaaggag atcagcatca aggatccggc
     1141 gggacacttc gtgacgttcg cgcagtttgg cgcagcccag cactgaggcc tgcccatcct
     1201 cgacccgcta ggcatgaaaa agggcgcgcg cgcgcgcgcc cattcgagga ggcaactcaa
     1261 tgacagaagg caaatctgaa ttcaatgcgc cggaagccgg ccagcggaag cccgaagccg
     1321 atcttaccgc ccgccaaacc cggccgattg catcgactgc cgcgagatgt cggaagtcga
     1381 cgcgatcccc tgcatgcgca gcttgaagtc gtcggcattc gacgcgttct ccagagccgt
     1441 ttcgtagttg accaaacctg cttggaacag atcccagagc gactggtcga atgtctgcat
     1501 gccgtattgc gaggtgccgg ctgcaatggc gtcacgaatg gcgcgcgttt tttcggggac
     1561 cagcacgcac tcacggatgt aagcggtatt gatcatcact tcgaccgcgg gcacgcggcc
     1621 ttcggcgtcg cagcgcctga cgaggcgctg acttacgatg gcgcggagca cagccgcgag
     1681 ctgcatgcga atctgcttct gctccggcgg cgggaaaatc gagatcacgc ggttgatggt
     1741 ttcgaccgcg tccagtgtgt gcagggtcga gaacaccatg tggccggttt cggccgcgtg
     1801 cagcgcggtc gatatcgttt ccaggtctcg catttcgccc accaggatga cgtccggatc
     1861 ctggcgcaga gaagcgcgca gcgccgcccc gaacgagggc gtgtcgacgc cgacttcgcg
     1921 ctggttgaca tagcctttct tgtcgcgatg caggaattcg atggggtcct cgatggtgat
     1981 gatgtgctcg gcgcgcgagg agttcacgcg atcgaccatc gcggccagcg tcgtagactt
     2041 accggacccg gtgacgccgg tcacgagcac cagaccgcgg ggcatgtcgc aaatctgctc
     2101 gacgaccttg ggcagaaaga gctcgtcgag agcgcgaatc ttggtgggaa tgacgcggag
     2161 caccaatccc acgttgccgc gctgctggaa gatgttcaca cggaagcgtc ccaggccggc
     2221 gacgccgtac gccatatcaa tttccgtggt ttccttgaat ttctgcttct gccgcgccga
     2281 catcatgctg aaggccatgt tcagcatatc ttcggcgctg atgcggggct ggtcagtcaa
     2341 cggcaccagt tcgccgtcga tgcgcaggtg agg
//
LOCUS       DASPUL010000585         5904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1478955, whole genome shotgun sequence.
ACCESSION   DASPUL010000585 DASPUL010000000
VERSION     DASPUL010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478955"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="VEJ00_13635"
     CDS             complement(<1..1328)
                     /locus_tag="VEJ00_13635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXX02253.1"
                     /translation="MAKIAVLYDAGQAVLSTFDLDEVLQRILGIIRDYFHLENAAILL
                     LDKELGQLCVRSQIGWDAGKEGIRLNPADGLTGASVEKKQPVYAPDVTKDSRYICSAQ
                     STRSELAIPLMVRDEVVGVLDCQSDQVNYFDPETINLLTLFSTQASIALQNARLYSLE
                     RERARQLEAINTIAKQSTAVMELEELLRRVCSVIRQAFRVAHVSLLLRDEGDLVLRAH
                     EGNLTPCMPSGGRFPADREPWSRVLATSGTLIEKNLSEASRLFRESASRMSIPLISFG
                     QTLGVLTLHSAERDAFRESELQSLESVADICANSIQNAHYVERVKQLAYLDGLTGIFN
                     RRFFEMRIGEEIERARRYGNGLAVMIADIDHFKKLNDEFGHLLGDEVLRQVSSLFHQQ
                     VRKIDVVCRYGGEEFAILLTQTSAPQAMIVAEKLRRMVEGWQFPGVARTVT"
     gene            complement(1417..1620)
                     /locus_tag="VEJ00_13640"
     CDS             complement(1417..1620)
                     /locus_tag="VEJ00_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02254.1"
                     /translation="MSKSSVVVNFIQALLAIVLGNVVYFVLAPSFPLAARHRPFHVDL
                     GLVIDFWFCLVAYGLIRTARRWR"
     gene            1777..4050
                     /gene="recG"
                     /locus_tag="VEJ00_13645"
     CDS             1777..4050
                     /gene="recG"
                     /locus_tag="VEJ00_13645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXX02255.1"
                     /translation="MLELSTPVQYVKGIGPRIADALAAKGIHTVGDLLHYLPFRYEDR
                     LNPRGIAELRPGEMATVIGEVRNSGLFRTRRMPIFQLTVGQGRARLRCLWFHATYLQD
                     KFKPGQMIALYGKVEEDQRSRELQIIQPQFEILGDAADEAENGGAAKKAAESLEIGRI
                     VPIYESIGQGHLTPRWFRRVLHTALENLTPDLQDPIPAVVRSRLGLISPRAALWNVHW
                     PEPGESLQDLQSRRTPAHIRLIFDELFFVELGLELKRRQQKAQTGIAFRLDDAVRAAI
                     KKILPFHPTAAQKRVLKEIATDMEKPCPMRRLLQGDVGSGKTIVGFEAAIIAIENGCQ
                     VALMAPTEILAQQHYFSARRILENAGYRIVLLTGSLETDRKREIRRHIAQGNAQLVIG
                     THALLEEKVEFARLGLVIVDEQHRFGVMQRLKLMKKSGDSESAAEGPSPVETAHVGTV
                     RVGTGASPVQPSAARRTPPQAPPPEPDVLVMTATPIPRTLALTLYGDLDLSVLDELPP
                     GRTPIVTKCVADDQSPKVWDFVRKQVEAGHQAYVVYPVISENEESELKAAIKMYRELS
                     SHVFADRKVGLLHGRLDADLKDQVMRMFQKGELQILVATTVIEVGVDVPNATVMVIEH
                     AERFGLAQLHQLRGRIGRGAAKSYCVLMTGGKVTEEGERRLQAMVDTNDGFKIAELDL
                     ELRGPGEFFGTRQAGMPSFRVANLIRDAQLLEAAKREAAAVLAGPNSEISSEEISRAL
                     VHMRALWHHTYGLVEVA"
     gene            4154..5074
                     /gene="lipA"
                     /locus_tag="VEJ00_13650"
     CDS             4154..5074
                     /gene="lipA"
                     /locus_tag="VEJ00_13650"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010331747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXX02256.1"
                     /translation="MAGASQLIQIELSPPLRAPKPSWLRAKAPVGDNFHNLKKLARGL
                     GLHTVCESAQCPNIGECWNHKTATFMLLGDICTRRCGFCAVPKGRPEPIDWDEPRRVA
                     EAVATLGLKHAVVTSVNRDDDNIGSARIFAETIREIRELTPDCRIEVLIPDFQGVEES
                     LRIVLAARPDVLNHNTETVPRLYRAVRSGARYERTLTLLENAKKFSSGMVTKSGVMVG
                     LGESTEELVEVFRDLGARGVDILTVGQYLRPSKDHLPIARFYEPEEFAYLRNEALRFG
                     FRHVESGPLVRSSYHAHEQADATASPRAVF"
     gene            5180..>5904
                     /locus_tag="VEJ00_13655"
     CDS             5180..>5904
                     /locus_tag="VEJ00_13655"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXX02257.1"
                     /translation="MVTILLVILGFCAGLLGALTGIGGGVLLTPILALHFGISIRQAI
                     GTSLVAVITTSAASSSVHLQRHTTDIRLGMTLELATSFGAAVTAYLVGYFNRNALEGL
                     FAGFLLYSAITILSRGGKVKSQDEPALPANGEAVIPPYEPQRYPLGLAASLVAGALSG
                     LLGIGGGPIKVPVMFIFMNVPLIVATATSNFMIGVTGAASAIVYYRRGDILVQYAAPL
                     AVGVFLGSLLGARLAPRIHTKIVV"
BASE COUNT         1194 a         2003 c         1610 g         1097 t
ORIGIN      
        1 gtcaccgtgc gcgctactcc cggaaattgc catccttcca ccatccttcg cagcttttcc
       61 gccacgatca tggcttgagg cgcgctcgtc tgggttagca ggatcgcgaa ttcttctccg
      121 ccgtagcggc acaccacgtc aatcttgcgc acctgctgat ggaaaagcga agacacttgc
      181 cgcaacacct cgtcgcccag caggtgtccg aattcgtcat tcaatttctt gaagtgatcg
      241 atatctgcga tcatcaccgc caggccgtta ccgtagcgcc gcgcccgctc gatctcttcc
      301 ccaattcgca tttcaaaaaa ccggcgattg aagatcccgg tgagcccgtc cagatacgcc
      361 agttgcttga cccgctcgac gtagtgcgca ttctggatcg agttggcaca gatatcggcc
      421 accgattcga gcgactgtag ctcactctca cgaaaggcat cgcgctccgc actgtgcagg
      481 gtaagcactc ccagagtctg gccaaacgaa atcagaggga tgctcatgcg ggaagctgac
      541 tccctgaaca gccggctcgc ttctgaaaga ttcttttcaa tcagcgttcc gctggtcgcc
      601 agaactcgtg accagggttc ccggtcagcg gggaaacgcc cccccgatgg catacagggc
      661 gtaaggtttc cctcgtgtgc cctcagcaca agatcgcctt cgtcgcgcag cagcagcgaa
      721 acatgggcca cccgaaacgc ctgccgaatc acgctgcaca cccgcctcag cagctcttcc
      781 agctccataa cggcggtact ttgcttcgcg atcgtattga tggcttccag ttgccgcgcc
      841 cgttcccgtt ccagcgaata caggcgcgca ttctgcaacg caatcgaggc ctgcgtcgag
      901 aacagcgtaa gcaggttaat cgtctcggga tcaaagtagt tgacctgatc gctctggcag
      961 tcgagcacgc ccacaacctc atcgcgcacc atcagcggaa tcgccagctc ggagcgcgtc
     1021 gattgcgcgc tgcatatata tcggctgtcc ttggtcacat ccggcgcata caccggctgc
     1081 ttcttttcca ccgaggcgcc cgtgagccca tcggcaggat tcagccgaat gccttccttg
     1141 cccgcatccc agccaatctg cgaacgcacg caaagctgtc ccaactcctt gtcgagcagg
     1201 aggatcgcag cattctccag atggaagtag tcgcggatga tccccaggat gcgctgcaat
     1261 acctcgtcca gatcaaaggt cgagagcacc gcctggcccg catcgtacaa gaccgcaatc
     1321 tttgccattc caacggattc taaacttcag gcgggtaacc accgaggttc cgaaagtaac
     1381 cagtttttcc ggcagcgcag ccgactttcg cccgcgctac ctccatctcc tggcggtgcg
     1441 aatcagaccg taagccacca ggcagaacca gaaatcaatg accaggccca ggtcgacatg
     1501 gaatggccgg tgccgggctg cgagcggaaa cgacggagcc aagacaaagt acactacgtt
     1561 tcccagcacg atcgccagca gcgcctggat gaagttcacg accaccgacg atttagacac
     1621 cgcggctccc ctatgcggtt gtcgctgctg gacaacgctg cgtgccggct cctgttacga
     1681 tgaggagcga ttgtcgattt ctcgatgtct cgattgttga aaggaagcct ggcatcgccg
     1741 gcgctttcat tcatcaatca acaatgattc tggctcatgc tcgagctctc cacacccgtg
     1801 cagtacgtca aggggatcgg tccgcgcatc gcggacgccc tcgccgccaa gggaatccat
     1861 acggttggcg atctgctcca ctatttgccg ttccgctatg aagaccgcct gaatccgcgc
     1921 ggcatcgccg agctgcgccc gggcgaaatg gcaacggtga tcggcgaagt gcgcaactcg
     1981 ggcctgttcc gcacccgccg catgcccatc ttccagctca ccgtgggaca ggggcgcgcc
     2041 cgcctgcgct gcctctggtt ccacgcgacc tacctgcagg acaaattcaa gcccggacag
     2101 atgatcgcat tgtacggcaa ggtcgaagag gatcagcgca gccgcgaact gcagatcatc
     2161 cagccgcaat tcgaaattct cggagacgcg gcggacgaag ccgaaaacgg cggcgcagcc
     2221 aagaaggcgg cggagtcgct cgaaatcggc cgcatcgtcc ccatctacga atccatcggc
     2281 caggggcacc ttacgccgcg ctggtttcgc cgcgtcctcc acactgccct cgagaacctc
     2341 acgcccgatt tgcaggatcc aattccggca gtggtccgca gccgcctggg gctgatctct
     2401 ccccgcgccg cattgtggaa cgtgcactgg ccggaacccg gagaaagctt gcaggatttg
     2461 caatctcgcc gaactcccgc ccacatccgc ctcatcttcg acgaactgtt cttcgtcgaa
     2521 ctcggcttgg agctgaagcg ccgccaacag aaagcgcaga ccggaatcgc atttcgtctc
     2581 gatgacgcag taagagccgc aattaagaag attctgccct ttcatcccac cgcggcccag
     2641 aagcgtgtcc tgaaggaaat tgctaccgac atggaaaagc cttgtcccat gcggcgtttg
     2701 ctgcagggcg acgtcggctc cggcaagacg atcgtcggtt tcgaagcggc catcatcgcc
     2761 attgaaaacg gatgccaggt cgcgctcatg gctcccaccg aaatcctcgc acagcagcac
     2821 tatttttccg cccgccgcat ccttgagaac gccggctatc gcatcgtcct cctgaccggc
     2881 tccctcgaaa ccgaccgcaa acgcgagatc cgccgccaca tcgcgcaagg caatgcgcaa
     2941 ctcgtcatcg gaacgcacgc gcttctggaa gaaaaagtcg aattcgcccg gctgggcctg
     3001 gtgatcgtcg acgagcaaca tcgcttcggc gtaatgcagc gcctgaagct gatgaaaaaa
     3061 tccggtgata gtgaaagcgc cgccgaaggc ccaagccctg tggagacggc ccatgtggga
     3121 acggtccgtg tggggacggg cgcctcgccc gtccagccaa gcgcagcgag gcgcacccca
     3181 ccacaagccc cgcctcccga acccgacgtt ctggtcatga ccgccactcc catcccgcgc
     3241 acgctggcgc tcaccctcta cggcgatctc gacctcagcg tgctcgacga gcttccgccc
     3301 ggccgcaccc cgatcgtcac gaagtgcgtc gccgacgatc aatcccccaa agtctgggac
     3361 ttcgtccgca agcaagtcga ggcaggtcac caggcttacg ttgtctatcc agtgatctcg
     3421 gagaatgagg aatccgagct caaggcagcg atcaagatgt accgcgaact gagcagccac
     3481 gtcttcgccg accgcaaagt gggcctgctc cacggccgcc tcgacgccga cctgaaagac
     3541 caggtcatgc gcatgttcca gaaaggcgag ttgcagattc tggtcgcgac caccgtcatc
     3601 gaagtcggag tcgacgtccc caacgccacc gtcatggtca tcgagcacgc ggagcgcttc
     3661 ggcctcgccc agcttcacca gctgcgcggc cgtatcgggc gcggcgctgc caaatcctat
     3721 tgtgtcctca tgaccggcgg caaagtcacc gaggaaggcg agcgccggct gcaggccatg
     3781 gtcgatacca acgacggatt caagatcgcc gaactcgacc tcgaacttcg cggccccggc
     3841 gaattcttcg gcacgcgcca ggccgggatg cccagcttcc gcgtcgccaa ccttatccgc
     3901 gacgcgcaat tgctcgaggc cgccaagcgc gaagccgccg ccgtgctcgc cggtccgaat
     3961 tcagaaatat cctcggaaga aatcagccgc gcgctcgtgc acatgcgcgc actgtggcac
     4021 cacacctatg gactggtgga ggtagcgtag cgcgtaagtc agctctctac gatgcctgtt
     4081 gcctcacaca atacaactgc cctgccaccc acgcaccaaa tgaaaatgcc aacaactggt
     4141 agaatcgaaa acgatggcag gcgcctctca actcattcag atcgagctca gcccaccgct
     4201 ccgcgcgccg aaaccgtcgt ggctccgggc gaaagcgccc gtaggcgaca acttccacaa
     4261 tctgaaaaag ctcgcccgcg gacttggcct gcataccgtc tgcgagtcgg cgcagtgccc
     4321 caatatcggc gagtgctgga accacaagac cgctaccttc atgctcctgg gcgacatctg
     4381 cacccgccgc tgcggcttct gcgccgttcc caagggacgc ccggagccga tcgactggga
     4441 cgaaccccgc cgcgtagccg aagccgtcgc cacactcggg ctgaaacatg cggtcgtcac
     4501 cagcgtgaac cgcgacgacg acaacatcgg cagcgccaga atcttcgccg agaccatccg
     4561 cgagattcgc gagttgacac ccgactgccg catcgaagtg cttattcccg attttcaggg
     4621 cgtggaagaa tccctccgca tcgtgctcgc cgcccgcccc gacgtgctca accacaacac
     4681 agaaaccgtc ccgcgcctct accgcgccgt ccgctccggc gcccgctatg aacgcaccct
     4741 gaccttgctg gaaaacgcca agaaattctc gtcgggcatg gtgaccaaga gcggtgtgat
     4801 ggtcggcctc ggagagtcca cagaagaatt ggttgaagtc ttccgcgatc tgggcgcgcg
     4861 cggtgtcgat atcctcaccg tcggccagta tctccgcccc tccaaagatc acctgcctat
     4921 cgcgcgtttt tacgagccag aagagtttgc ttatctgcgg aacgaagccc tgcgcttcgg
     4981 tttccgccac gtcgagtccg gaccgctggt gcggtccagc tatcacgcgc acgagcaagc
     5041 cgatgccacg gcgtcaccgc gggcggtgtt ttagcttgaa gccgacgggg tctcccgcca
     5101 gattcctctt cctcgacacc gcggttctta gggtcactat ctccatcgga aaaatcggca
     5161 agcggaggtt cacccccgct tggtaactat tcttcttgtt attctcggat tctgcgccgg
     5221 cctgctgggc gcgctgaccg gcatcggcgg cggcgttctg ctcacaccca ttctcgccct
     5281 gcacttcggc atttccatcc gtcaggccat cggaaccagc ctggtcgcgg tcatcaccac
     5341 ttcggctgcc agttcttccg tccacctgca gcgccacacc accgacattc gtctgggcat
     5401 gaccctcgaa ctggcgacct cgttcggcgc tgccgtcact gcctatctgg tgggatactt
     5461 caaccgaaac gctctcgaag gcctgttcgc cggattcctc ctctacagcg cgatcacgat
     5521 tctgagtcgc ggggggaaag taaagtcgca ggacgaaccg gccttgccgg cgaacggcga
     5581 ggccgtgatc ccgccttacg agccgcaacg ttatccgctg ggactggccg cctcgctggt
     5641 cgcaggcgca ctctccgggc tgctcggaat cggcggaggc cccatcaagg ttccggttat
     5701 gtttatcttc atgaatgtgc ccttgatcgt ggctactgcg acttcgaatt tcatgatcgg
     5761 cgtcaccgga gccgccagcg ccatcgttta ctaccgccgc ggcgacatcc tggtgcagta
     5821 tgccgcgccg ctcgccgtcg gagtattcct gggatccctg ctgggcgcac gcctggctcc
     5881 gcgcatccac acgaagatcg tcgt
//
LOCUS       DASPUL010000586         2891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1336378, whole genome shotgun sequence.
ACCESSION   DASPUL010000586 DASPUL010000000
VERSION     DASPUL010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336378"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1361
                     /locus_tag="VEJ00_13660"
     CDS             96..1361
                     /locus_tag="VEJ00_13660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX02258.1"
                     /translation="MPRNRYMVVIVFLTFFVMSLLTNILGPIVPDIISSFHVGLAAAA
                     FLPFSFFIAYGVMSIPAGFLVERFSEKPVMIAAFLAGTAGSLSFALHPSYRVAVASLF
                     VMGSGMATLQTAINPLLRVAGGEEHFAFNSALAQLIFGSASFLSPYMYSYLVINLAGA
                     PVHKNVVLAVLAQLTPPALPWASMYWIFAVFTLLMIVVLYFSRFPQVEHTAEEQAGSW
                     EMYGSLFRKPMVWAFFFCVFAYVGSEQGTADWISKFLSQYHGYDPHTTGAAAVSWFWG
                     LLTAGCLIGMLLLKIFDSRYVLRGTCVGALLCLTAALFGPARVSLIAFPAIGLFASVM
                     WPILVSLALNSVAEHHGSFAGILSTGIMGGAVVPVIIGRIGDHFGLRAGVAFLYLTFA
                     CVFSVGFWARPIITNATITLKKTVAEPVS"
     gene            1415..2362
                     /locus_tag="VEJ00_13665"
     CDS             1415..2362
                     /locus_tag="VEJ00_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02259.1"
                     /translation="MLTVVMCASGTAWAQSDPQTPPKGVPYLRLSAPRHVLPDPQKWE
                     GDQFPHTLSVLELRRDGFRYWGWYGLNEGRGIGLARSNDLVNWIKYENNPLWTNARWP
                     AVLAKADPAHKDFLYFAITRDYDTPGSYIVLASSKDGIHLTEEKVLVAKVPDQRNQNP
                     NLFRDPKSGRFYLTFYRGNDHDRFEIVSKSATSVRGLDAAPEKVLLRSNETVAAPTLL
                     WVKSAKTASGHKGLYYLATEIYPHRYTDDPEGEWQVKVFAADAPDGNFQPVDGDPVMG
                     GQRACLFQHIFNGRFYGYDCHLDTSTDRWMLEEVEAALP"
     gene            2412..>2891
                     /locus_tag="VEJ00_13670"
     CDS             2412..>2891
                     /locus_tag="VEJ00_13670"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXX02260.1"
                     /translation="MTKIKKSQIILNVHPLLEKLIHSIVSAPWTALLLTFLFSVGMSS
                     SAMAQLYAGSVTGSVKDSTGAVISGATATLVDEQKGFSFSATTDATGRYLFRSVSPAT
                     YRLSISASGFKKEDRAGIRVDVNQNVSADFALQVGAGSETVNVTAAAPLLATQDAVTG
                     "
BASE COUNT          543 a          831 c          840 g          677 t
ORIGIN      
        1 cgctcgctct ggcggcgcag gtaggccgcg agcccggccg cttcgagctg gcttcgaaag
       61 ttacgacgag ggaatagaag aagatgtcgc gctccatgcc cagaaaccgc tacatggtcg
      121 tcatcgtctt cctgacattc ttcgtaatgt cactgctgac gaacattctc gggccgatcg
      181 tacccgacat catcagcagc ttccacgtag gacttgcggc tgctgccttt ttgccgttct
      241 ctttttttat cgcctacgga gtaatgtcga ttccggcggg ttttctcgta gagcgtttca
      301 gcgagaagcc ggtcatgatc gcagctttcc tggccggaac cgctgggtcg ctcagttttg
      361 cgttgcatcc gagttaccgg gtggcggtag cttcgctatt tgtcatggga tcgggcatgg
      421 ccaccttgca gactgcgatc aatccgttgt tgcgcgtggc cgggggagaa gaacatttcg
      481 cctttaattc cgctttggcg caattgatct tcggctcggc atcgtttctc agcccctaca
      541 tgtactcata cctggtgatc aatctggcgg gcgcgccagt ccacaagaat gttgtgttgg
      601 cagtactggc gcagctgacg ccacccgctc ttccctgggc ttccatgtac tggatttttg
      661 cggttttcac attgctgatg atcgtggtcc tctacttcag ccgtttcccg caggtcgaac
      721 acacggcaga agagcaggct ggatcctggg aaatgtacgg aagcttgttc cgcaagccca
      781 tggtgtgggc cttcttcttt tgtgtctttg cttatgtcgg ttcggagcag ggaactgccg
      841 actggatttc gaagttccta tcgcaatatc acggctacga tccacatacg acgggtgctg
      901 ctgccgtttc ctggttctgg ggcctgctca ccgccggctg cttgatcgga atgttgctgc
      961 tgaagatttt tgacagtcgc tatgtgctgc gcggtacctg cgtcggagca ttgctgtgcc
     1021 tgacggctgc tctctttggt ccggcgcgag tttcgctgat cgcttttcct gcaatcggac
     1081 tgttcgcctc ggtcatgtgg cccattctgg tttcgcttgc tctcaattca gtcgccgagc
     1141 atcacggttc gtttgccggc atcttgagca caggaatcat gggaggggct gtcgtacccg
     1201 tcatcatcgg ccggatcggg gatcacttcg gcttgcgcgc cggtgtggca tttctttatc
     1261 tcacgtttgc gtgcgtcttc agcgtcggct tctgggcgag gccgatcatc accaacgcga
     1321 ccatcaccct gaagaagacg gttgccgagc cagtttcgta ggaagggaag cgggagggga
     1381 cccgatgttc acaaatcttt gcgctagagg aatcttgctg acggtcgtta tgtgcgcctc
     1441 gggaactgcc tgggcgcaga gcgatcctca aaccccgccg aagggtgtcc cgtacttaag
     1501 gcttagcgct ccccggcacg tgctgcccga ccctcaaaaa tgggagggtg atcagttccc
     1561 ccacaccctg agtgttctcg aactgcggcg cgatggattc cgctactggg gctggtacgg
     1621 gttgaacgag ggaaggggaa ttggtttggc gcggagcaac gatctcgtga actggatcaa
     1681 gtacgagaac aatccgctat ggacgaacgc acgctggccc gcggtcttgg ccaaggccga
     1741 tccagcacac aaagactttc tctattttgc gatcacgcgt gactacgaca cgccgggcag
     1801 ttacatcgtg ctggcctcgt cgaaagacgg tatccatctc accgaagaga aagtccttgt
     1861 ggcgaaggtt cccgatcagc gcaaccagaa tcccaatctt ttccgcgacc cgaagtctgg
     1921 gcgcttctat ctgacctttt atcgcggcaa cgaccacgac cgtttcgaga tcgtcagcaa
     1981 gagcgcgacc agcgtgcggg gcctggatgc ggcaccagaa aaggtcctgt tgcgctccaa
     2041 cgagaccgtc gctgccccca cgctgctctg ggttaagagc gcgaagactg ccagcgggca
     2101 caaaggcctc tactacctgg cgacagaaat ctacccccat cgttacaccg acgatcccga
     2161 gggggaatgg caagtaaagg tatttgctgc agacgctccc gacggcaact tccagccagt
     2221 cgacggcgat ccggtcatgg gcggccagcg cgcctgcctg tttcagcaca ttttcaacgg
     2281 ccgtttctac ggttacgact gccatctgga tacttcgacg gaccgatgga tgctcgagga
     2341 ggtcgaggct gcgctgccat gactgcagcc ggcctggcga gcgatttagt tgatggataa
     2401 ggaggactct tatgacgaag atcaaaaagt cccagatcat attgaacgtt catcctttgc
     2461 tggaaaagct gattcactcg atcgtcagtg ctccgtggac ggccctcctg ctgaccttcc
     2521 tgttttcggt tggaatgagt agctcggcga tggcccaact ctacgccgga tccgtgaccg
     2581 gatcggtcaa ggactccacg ggagcagtca tctccggggc aaccgccacg ctcgtggatg
     2641 aacagaaggg cttctccttt agcgcgacta ccgatgccac cgggcgctac cttttccgga
     2701 gcgtctcccc ggcaacgtat cggctctcca tcagtgcgtc tggtttcaag aaggaagatc
     2761 gggcgggaat tcgcgtggac gtcaatcaaa acgtctcggc agatttcgcc ctgcaggttg
     2821 gcgcgggctc agagactgtg aatgtcaccg cggcggcgcc gctcctggct acgcaagacg
     2881 ccgtcaccgg a
//
LOCUS       DASPUL010000587         2211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_790947, whole genome shotgun sequence.
ACCESSION   DASPUL010000587 DASPUL010000000
VERSION     DASPUL010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790947"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1538
                     /locus_tag="VEJ00_13675"
     CDS             33..1538
                     /locus_tag="VEJ00_13675"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HXX02261.1"
                     /translation="MKTTISLSAPAEQETESLVVVVLDHAEPVPGDKDKKPQLKVATT
                     DTAVQSVAADLLASGEVAGKPFETNLLHKPAGLKAKRLLLISGGSAKKFSSYDLRRAA
                     GTAVRTLKSRGIRSFAFVAPPAIAAEEAVRAIVEGAHVGNFDPDYYRSDRKDQRIDGL
                     TVIAGGDKTQLENAAAEAQVVGESQNFTRDLVNEPSNRMTPTILAERAKKMCAEVGLR
                     CELYGADKIKEMKMGAFWSVAQGSDEPPALIVMKYEPAGAPEKPVLGLVGKGITFDTG
                     GISIKPADGMEKMKYDMAGGATMIGAMRAIALLKPKVKVIGIVCATENMPSGHAQKPG
                     DVQIAMSGKSIEIINTDAEGRLVLADGLYYARQLGCTHLVDAATLTGAVVVALGYANA
                     GIFANDDDVYNRFHTANARAGEKMWRMPLDDEYKEQIRSSIADIVNSGGRWGGAVTAA
                     MFLKEFAEDTPWIHLDIAGTAWMEDQKPWIAKGPSGIALRSLVEFVKGFAQ"
     gene            1790..1865
                     /locus_tag="VEJ00_13680"
     tRNA            1790..1865
                     /locus_tag="VEJ00_13680"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1823..1825,aa:Phe,seq:gaa)
BASE COUNT          500 a          646 c          662 g          403 t
ORIGIN      
        1 tggtacttca ttcgaaggct tccccgaaaa tcatgaaaac tacaatttct ctctcagccc
       61 ctgccgagca ggaaaccgag tccctggtcg tcgttgtcct tgaccacgca gagccggtgc
      121 ctggagataa agacaaaaag ccacaactga aggtcgcgac aacggacacg gccgtgcagt
      181 cagtggcagc cgatctgctg gccagcggcg aggtcgccgg caaacctttc gaaaccaacc
      241 tgctgcacaa accagccgga ttaaaagcga agcgactgct gctaatcagt ggcggcagcg
      301 cgaagaagtt ctccagctac gatctgcgcc gtgctgcggg aacggcggtt cgaacgctga
      361 agtcacgcgg gatccgcagc ttcgcttttg tcgctcctcc cgccatcgcc gcagaggaag
      421 ccgttcgcgc gattgtggaa ggcgcacacg tgggcaattt cgatcccgac tactaccgga
      481 gcgaccgcaa ggatcagagg atcgacggat tgaccgtcat cgccggcggc gacaagactc
      541 agttggagaa tgcggccgcc gaggcgcaag tcgtggggga atcgcagaac ttcacgcgcg
      601 acctggtcaa tgaaccttcc aatcgcatga cgcccacaat cctcgcggag cgggcaaaga
      661 aaatgtgtgc cgaggtcggc ctgcggtgcg agctatacgg tgccgacaag atcaaagaga
      721 tgaagatggg ggccttctgg agcgtcgccc aaggatccga cgagccgccg gcgctgattg
      781 tcatgaaata cgagcccgcg ggagctcctg aaaagccggt cctgggcctg gtggggaagg
      841 gaattacctt cgataccggc ggcatctcga tcaaaccggc tgatggcatg gagaaaatga
      901 aatacgacat ggccggcggc gccaccatga ttggtgccat gcgcgccatc gccttgctca
      961 agccgaaagt caaggtcatc ggcatcgttt gcgcgaccga gaatatgcct tccggccacg
     1021 cgcagaagcc cggagacgtg cagatcgcca tgtccggcaa atcgattgag atcatcaata
     1081 ccgacgcgga aggacgtctg gttcttgccg acggcctcta ctacgcccgc cagctcggat
     1141 gcacgcatct ggtcgacgcg gccacgctga caggcgcagt cgtggtggcg ctcggctatg
     1201 cgaacgcagg aatcttcgcc aacgatgacg atgtgtacaa ccgcttccac actgcaaatg
     1261 caagagcggg tgagaagatg tggcgcatgc ctctcgacga cgaatacaag gaacagatcc
     1321 gttcgtcgat tgccgatatc gtcaattccg gtggacgttg gggcggagcg gtgactgcgg
     1381 ccatgtttct gaaggagttc gcggaagaca ctccgtggat ccatctcgac atcgccggca
     1441 ccgcctggat ggaagatcag aaaccctgga tcgcgaaggg cccctccggg atcgcgttac
     1501 ggagcctcgt cgagttcgta aagggcttcg ctcagtaatt cgaccgccgg gaattgggcg
     1561 cagtccgcac gccacgcgag ccgcaccgag cgcgtaccac cgatgtgctt gatcgccgat
     1621 tgtcttttgt cgtccagccg gcgacattga gcgaactcag taccgttgct ataatcgaga
     1681 gcgttccaaa cgcttcccgc aacggattca tttggttcgg tcggcaatcc cgccacaacc
     1741 gcgtcgagcg gatagcgttc gatcccgcac acggcgggtt tcgcaaggcg gccaggtagc
     1801 tcaggtggta gagcgcagcc ctgaaaaggc tggcgtcggc ggttcaactc cgtccctggc
     1861 caccatgttt tcaacgacgt agctcgtcgt cccacctgac tttggctcca attgctccaa
     1921 aataatttcc aactagctag ttcaccatcc agcaccactg taaggagctc accacgggcg
     1981 aggaagcctg tgatgtggat cactgtgtgc cacccggagt ttaaatagga tactcgcaac
     2041 atccttggcc ttaagcggtg aggtcaaagg aattcaacag ctgcagcacg aagagggagc
     2101 aggccatgcg cgccccggca tcctgcgagg aagtcacttc gaatgcgagc tgggatgcag
     2161 ccgagcggga attagactat cggccgggtg atcccatcaa cattggctgg a
//
LOCUS       DASPUL010000588         4722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_254148, whole genome shotgun sequence.
ACCESSION   DASPUL010000588 DASPUL010000000
VERSION     DASPUL010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254148"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..501)
                     /locus_tag="VEJ00_13685"
     CDS             complement(124..501)
                     /locus_tag="VEJ00_13685"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HXX02262.1"
                     /translation="MQLSTRANGRHERMHLTLKQQATRPAGANFLQQQAKFDAFQEEF
                     NHERPHEALAMKCPAEIYSASCRPYLGIPEPQYPFHDRTVAVTSCGRLCLYRKKINLS
                     TSLAGQAVGIKEVDHGIWLEAIS"
     gene            525..791
                     /locus_tag="VEJ00_13690"
     CDS             525..791
                     /locus_tag="VEJ00_13690"
                     /inference="COORDINATES: protein motif:HMM:NF036442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5372 family protein"
                     /protein_id="HXX02263.1"
                     /translation="MFRVIHPFHPWHGRRFELIAYKSAWGEDRVYFHNEEQQLIALPA
                     SWTDVVAVDPFVSIAQGRALFRADDLFELVGFVRGLSSRGGNHV"
     gene            1040..1426
                     /locus_tag="VEJ00_13695"
     CDS             1040..1426
                     /locus_tag="VEJ00_13695"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXX02264.1"
                     /translation="MEHESFRGSEFFDPEDLLQVKYEMLRQVDVDKQPVSQAAKRFGF
                     SRPSFYQAQAAFQEAGLAGLLPQKRGPRSGHKLTPELMQFVTQLRLAEPEISSAQIAE
                     RIAQRFGVSVHPRSIDRQLRHQKKLR"
     gene            1761..3806
                     /locus_tag="VEJ00_13700"
     CDS             1761..3806
                     /locus_tag="VEJ00_13700"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HXX02265.1"
                     /translation="MAYLYIRQSTLRQVFENVESTKRQYALRQHAVALGWQPDQIVVI
                     DTDLGQSGASATDREGFQRLVTEVSLGRAGIVLGLEVSRLARNSMDWHRLLEICALAD
                     TLILDEDGVYDPAHFNDRLLLGLKGTMSEAELHVLRARLQGGILNKARRGELFMRPPI
                     GFAYDALGRVVLDPDQQIQHTIRLLFDTFQRTGSAMATVREFRNTGVLFPRRIHSGPG
                     KGDVIWGKLEHSQVLRVLHNPRYAGVFVYGRTRTRKSVEGECRVQHLPREEWHTFLPE
                     SHPAYLSWQEYERNLKRLRESAQAIGFERRKSPPREGPALLQGLIVCGKCGRRMTLRY
                     HVRQAGLCPEYVCQRKGIENAEPPCQRIPGAEVDRVISDVLLELVNPVTLDVALTVQH
                     ELQARLDESDRLRKQHVERARYEAELAQRRYMRVDPENRLVADTLEADWNQKLRALAE
                     AQEEYERRREQDRQLFNEEQRAAIMALAQDFPRLWRDPATEDRDRKRMIRLLVEDVTM
                     IRGQEITLHLRFRGGAARTVTLPNPLRSWESWMTDAEVVCKIDQLLNAQTFGRIAATL
                     NADGFRSGKGQRFTPRYIARIQKHYALRSRFDRLRALGLLTLNEMASVLSVNPKTVKI
                     WTAHGLLKAHAYTDKPEHLYEPVGSAMPRKAQGVKLSVRRATASLLPQCSKEVQCEA"
     gene            complement(3830..4552)
                     /locus_tag="VEJ00_13705"
                     /pseudo
     CDS             complement(3830..4552)
                     /locus_tag="VEJ00_13705"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1003 a         1325 c         1401 g          993 t
ORIGIN      
        1 atcgaacgcc tgtttgcttg gttgcataac tttcgccgtc tggtcacccg ctgggagtat
       61 cacgaggcca acttcctcgg tctggtccag ctcggttgcc tcctcatctt gatgaggaac
      121 tatttatgag atggcctcta gccagatgcc gtggtcgact tccttgatac cgacggcttg
      181 gccggcaagg gatgtgctga ggttgatctt tttgcgatac aggcaaagac ggccgcagct
      241 ggtaaccgcg acggtgcggt cgtggaaggg atactgaggt tccgggatgc ccagataagg
      301 gcggcaggaa gcggagtaga tttcggccgg gcatttcata gcgagagctt catggggtct
      361 ttcgtgattg aattcctcct ggaaggcgtc gaatttggct tgctgctgca ggaagttggc
      421 ccccgccggc cgggtggctt gttgcttcag ggttagatgc atgcgttcat gtcggccatt
      481 ggcccgcgtc gacaactgca ccgcaagacg acactgcggg acgagtgttc cgagtaattc
      541 accccttcca tccgtggcat ggccggcgct tcgaactcat cgcgtacaag agcgcctggg
      601 gtgaggaccg agtctacttc cataacgaag aacagcaact gatcgcattg ccagccagct
      661 ggacagacgt tgtcgctgtc gatcctttcg tttcgattgc ccaaggccgg gctctgtttc
      721 gggccgacga tctgttcgag ttagttgggt ttgttcgcgg gttgtcgtcc agaggtggca
      781 accatgtgta aagttaatta tgcctcttgt gtaaaggaaa agatgtcgag tgagtcgcct
      841 tcacctccgt gtttcttgac tatatctacc attcaacgct gcaatgatac tgacaaacga
      901 cagggccgtc cgcaaaaagg gcttgacatt aaatatgata tgtacataat tatatttaca
      961 gagaaacatg cccgactccc cgcaaaccaa gcgatccttt ctgcgccagc agggcacgtt
     1021 gaatccgcgt ccgaaggccg tggagcacga gagctttcgc ggcagcgagt tcttcgatcc
     1081 cgaagattta ctgcaggtca agtacgagat gctgcgccag gtggatgtcg acaagcaacc
     1141 cgtcagtcag gcagccaaga ggtttggttt ttcacgcccg tcgttctatc aggcccaggc
     1201 agcctttcaa gaagctggcc tcgccggcct gttgccccag aaacgcgggc cccggtccgg
     1261 gcacaagctc acccccgaac tgatgcagtt tgtaacacaa cttcggcttg ccgagccgga
     1321 gatctccagt gcgcagatcg ccgaacgaat cgcacagcgc ttcggtgtct cagttcaccc
     1381 gcgcagtatc gatcggcaac tgcgccatca aaaaaaactc cggtgagtcc agagccatcg
     1441 gccgtttctt tggccgatcg ccggctggtc gcaggctacg aagagctacg atctcaagcc
     1501 gtacaaggat tgcggcgagg accgggtctg gcactgatca tggcccgtgg gttccgctgc
     1561 tggatggaag cctttagcca gcttctcgtt accaaaggca gccacccgca agtgccgcat
     1621 gggcctgcac ccgcaatgcc ttccggtctg cgcggggaac tggtgattct gcttgccagc
     1681 atactgctgc gccgagcttc gcaggagatc gtatgagagc ggacgcacat cagaaagtga
     1741 aacccagcca tctgaaacgg atggcttatc tctacatccg gcagtccacg ttacgtcagg
     1801 tcttcgaaaa cgtcgaaagc acgaaacggc agtatgcgct tcgccagcat gccgtggctc
     1861 tgggctggca gccagaccaa atcgtggtca tcgatacgga cttaggccaa tcgggagctt
     1921 cggccactga ccgggaaggc tttcagcgtt tggtcacgga ggtcagcctg ggacgtgcgg
     1981 gcatcgtgct cgggctggaa gtatcgaggc tggcacgaaa ctcgatggac tggcaccgct
     2041 tgctcgagat ctgcgcgtta gccgatactc tgatcctaga cgaagacggc gtctacgatc
     2101 cggcgcactt caatgaccga ctattgctcg gtctaaaagg cacgatgagc gaagccgaac
     2161 tccacgtcct gcgtgcacgg ctgcagggcg ggattctaaa caaggcccgg cgcggagagt
     2221 tatttatgcg gcctcccatc ggtttcgcct atgatgcgct gggacgggtg gttttggatc
     2281 cagaccagca gattcaacat acgatccgac tgctgtttga tacttttcaa agaactgggt
     2341 cggccatggc aacggtgcgg gagttccgca acaccggcgt attgtttcct cgccgcattc
     2401 attctggtcc aggcaaggga gatgtaatct ggggcaagct ggagcacagc caggtactgc
     2461 gtgtgctgca caacccgcgg tatgctggcg tgtttgtgta cggccgcacg cggacacgca
     2521 aaagtgtgga gggcgagtgc cgagtacaac acttaccgcg ggaagaatgg cacaccttct
     2581 tgcccgaatc tcatccagcc tatcttagct ggcaggagta tgaacggaac ctcaagcgtc
     2641 tgcgagaaag tgcgcaggcg atcggtttcg agcggcgaaa gagcccgccg cgcgaaggcc
     2701 cggcgctgct gcagggcctg atcgtctgtg gcaagtgcgg tcggcgaatg acgcttcgct
     2761 accacgtgcg tcaagcgggt ctgtgccctg agtatgtctg tcagcgcaag gggattgaga
     2821 atgccgaacc gccttgccag cgtattcctg gcgctgaggt ggatcgtgtg atcagtgacg
     2881 tgctcttgga actggtgaac cctgtcacgt tggatgttgc tctgaccgtg caacacgaac
     2941 tgcaggcgcg gctggatgaa tccgatcgcc tccgcaaaca gcatgtggaa cgtgcccggt
     3001 acgaagccga gctggcgcag cgccggtaca tgcgcgtgga tccggagaat cgattggtcg
     3061 ccgatacgct cgaagcagat tggaatcaaa aactgcgtgc actagctgag gcgcaagagg
     3121 aatacgagcg acgccgcgaa caggaccgtc agcttttcaa tgaggaacag cgcgccgcga
     3181 ttatggcact ggctcaggat tttccgcgac tttggcgaga tcctgccacc gaagatcggg
     3241 atcggaagcg gatgatccgc ttgctggtcg aggatgtcac gatgattcgt ggccaagaaa
     3301 tcacattgca tctgcgcttt cgcggaggcg ctgcaagaac cgtcactttg cccaatccgt
     3361 tgcgatcctg ggaaagttgg atgactgatg cagaagtagt gtgcaaaatc gaccagttgc
     3421 tcaatgcaca gaccttcggt cggatcgcgg cgacgctcaa cgcagacggc ttccgctccg
     3481 gaaaagggca gcgcttcact ccgcgctaca tcgcccgcat tcaaaaacac tatgcacttc
     3541 gctcgcggtt cgaccggcta cgcgcgctcg gcctgttaac gctcaacgaa atggcgagcg
     3601 ttctgagcgt caatccgaag accgtaaaga tctggactgc tcatggatta ctcaaggctc
     3661 atgcttacac ggacaaacct gagcacttgt atgagccggt tggctctgcc atgcctagga
     3721 aagcgcaagg cgtaaaacta tcagttcgcc gagcaaccgc ttcgctccta ccgcaatgtt
     3781 cgaaagaggt gcagtgtgaa gcgtaagcgt tctgctgggg gtggccgggc cggatccgct
     3841 caatggaaat tcctaaccgc agccaccata ccgagagttt ggagagctga aacagtccat
     3901 tcggagaggc aaagggaacg ccgttgtccg agcggatggc ccggggcagg ccgcgttcgt
     3961 ggaacaagcg ctcaaaggcc cgcaaagcga gttcttcccg gttagattcc agcgcttcgc
     4021 acagcagcag gtaacgcgag cagtagtcgg tgaccgtcag cggatagcaa taccgcttat
     4081 cgtgcaactg aaactcgccc ttgtaatcgg tgcaccagag atcgttggga tgcaggccat
     4141 cggacaaggg cgtaccttcg gctcgactgc ggcgccgacc catcggcttg accaggccgt
     4201 ggcgatccag cacagcgtga atcgtagagg cggccggagt cttgacggca tgcgacaggt
     4261 gccgcagcag acgctcccgg atcttgcgtg caccccagtg aggtttctcg cgcttggcgc
     4321 taaggatagc cgcctccacc ggctcgggta actggttcgc gtagcggtag ggccggcgag
     4381 ttcgatcact taacccttcc agtccacatt gctcgtaacg ctcaaagatc ttgtagccgg
     4441 tcttgcgtga gatgccgaac tcccggcaca gcgaagccat actctccccg tccttgagcc
     4501 ggatcacaaa ccacattcgt tcgtccataa cagagcgctc cttccatggc atcctccttt
     4561 tcgccccccc tttcgggccg aaaagtgtaa cctatgtctt cggaacgttc tctccagaaa
     4621 ctcgaagggc ccccaggcta ctaggccgca ggaatctgag ttaggcggaa gcccagtttg
     4681 gctgcttttc gttccaggtg atggacttgc cgttggtgaa tc
//
LOCUS       DASPUL010000589         4765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1208158, whole genome shotgun sequence.
ACCESSION   DASPUL010000589 DASPUL010000000
VERSION     DASPUL010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1208158"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..557
                     /locus_tag="VEJ00_13710"
     CDS             177..557
                     /locus_tag="VEJ00_13710"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HXX02266.1"
                     /translation="MDYLLKTEPSTYSFADLQREKTTIWDGVSNPVALKNLARMKPGE
                     RLVIYHTGDEKSAVGTASVVSVDVADPRNPQVKIKVGKANPKPVTLSEAKASVLFSES
                     PLVRQGRLSVVPLTEAQYKFLTGE"
     gene            complement(648..1001)
                     /locus_tag="VEJ00_13715"
     CDS             complement(648..1001)
                     /locus_tag="VEJ00_13715"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HXX02267.1"
                     /translation="MDDLTGLAAVVMLLGMPTAIVGMYLYYRVRKLRTEERLAAIQRG
                     VSIPMEPDLSEAARSRRAGILLVAGAVGYMLAFLILARFERDALMAASFGAIPFFLGL
                     GYFLDSALIRRDARA"
     gene            1184..2266
                     /locus_tag="VEJ00_13720"
     CDS             1184..2266
                     /locus_tag="VEJ00_13720"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HXX02268.1"
                     /translation="MKLLLRFRLRSLRAVAVYSLLIVAVQVLRAQETKEKVTVDDVER
                     SYTLRLPSGYDAQRRYPVVILLHGMNQDADDMERLTQFDALADKDGIVAVYPFALHGR
                     WNTGVHPQERRPMGMGPGGGRHRGGYPGGGGYPGGGGGYPGGGQPRSREPDEERRPAP
                     ADDIAFFNRMLDQLQSKLSIDASRIYVAGLSEGGFMSMRAACAMSDRVAAIAAVGAAM
                     PKTMICLPSRPVPVAMINGTSDPVVPYGGGTEHNLNLATLSAEDSAKAWAKIDRCEEK
                     PAHDKLPSREKGGMETKVDTYTACQQGAQVVLYSVKGAGNTWPGGEQYEAENTVGKTS
                     HDLNANETLWSFLVTKKMAAKNDSGK"
     gene            complement(2414..3799)
                     /locus_tag="VEJ00_13725"
     CDS             complement(2414..3799)
                     /locus_tag="VEJ00_13725"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HXX02269.1"
                     /translation="MRYRYVPLVVVGLAATLLCGPEVQSQNDPGPRPGPAAAGSFFPG
                     LNANEQTFFGQAQQRFQEVDSVSGGIAGETGSGLGPTFNGNSCAMCHAQPAVGGSSPG
                     LASPQHSIQNPQVALAILDSAGNTVPSFITANGPVREARFIRNPDGTRDGGVHDLYTI
                     AGRSDAVGCMLGQPDFATQLANNNVIFRIPTPLFGLGLVEATPDSILQANLASTQAAR
                     AAMRIGGTFNTSGNDGTITRFGWKAQNKSLLVFVGEAYNVEQGVSNELFPNERSAVAG
                     CVFNNSPEDASNMLNPNPSSPNYGTQVGTVSEMSSDVVNFAAFSRMSAPPTPAPPTTS
                     TQNGAALFNSVGCALCHSPSLTAGPSDYTGMSNITYHPYSDFALHHMGWNLADGIHQG
                     NAGADEFRTAPLWGLGQRLYFLHDGRTSDLIEAINAHGDRDSEANRVVRRFRTLNPSQ
                     MQDLLNFLRSL"
     gene            complement(4145..>4765)
                     /locus_tag="VEJ00_13730"
     CDS             complement(4145..>4765)
                     /locus_tag="VEJ00_13730"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXX02270.1"
                     /translation="PVTHVGFLDPALYHPESIGQPLAQTDCRVLAQAEIDPSDTPESI
                     TEVPSGCPGELVMRGPQFMLGYWKDPQATAAVLRDGWYWSGDIVTRDKEGFYRVVDRR
                     KEMIKYKGFAVAPAEVEAVLLEHPAVKECGVVGRPDAAAGEVPVAFVALRDGFVDSHK
                     LSEELCAFVADRLTHYKQPREVRFVEVVPKTASGKILRRELRKCIA"
BASE COUNT          980 a         1380 c         1511 g          894 t
ORIGIN      
        1 gccgccgaag caggatgctc caccgccgga tcagaaggaa caaaagccga actagggcgg
       61 attgaggttc gatttcagat ctgagattgc agacttcaga tcgcagcagc cgttcgatgg
      121 ccccatcgac aggaaagagc ctgaaaatct aaaatcgagc atcttaaaac ggatctatgg
      181 actacttgct caaaactgaa ccttcaacct actcgtttgc ggacctgcag cgggagaaga
      241 ccacgatctg ggacggggtg tcgaatcccg tggcgctcaa gaatctggct cggatgaagc
      301 cgggcgagag gctggtcatt taccacacgg gcgacgaaaa gagcgccgtg ggaacggcga
      361 gcgtggtgtc cgtggatgtt gccgatccgc ggaaccctca agtgaaaatc aaagtcggga
      421 aggcgaaccc caagccagtg actctgtcgg aagcgaaagc cagcgtgttg ttttcggagt
      481 ccccgcttgt gcgccaagga aggttgagcg tggtgccctt gaccgaggcg cagtacaagt
      541 tcttaaccgg ggagtaactt cacagtccca cttctcgcgc aaaaggcgcg cgagaaatgg
      601 gccacccgct tccaaaccct ctttttcctg ctagcggctc gcgtccttca ggcccgggca
      661 tcgcggcgaa ttagcgccga gtcgagaaag tagccgagcc ccaggaaaaa gggaatcgca
      721 ccgaacgagg ctgccatcag ggcgtcgcgc tcgaaccggg cgaggatcaa gaaggccagc
      781 atgtagccga cggcgcctgc caccagcaga atgccggcgc ggcgggaacg cgccgcctcg
      841 gaaagatcag gttccatggg aatgctgacg ccccgctgaa tcgccgccag gcgctcctca
      901 gtgcgcagct tgcgtacgcg atagtacaag tacattccca cgatcgcggt gggcatccct
      961 aaaagcatta cgacggctgc gagacctgtt aggtcgtcca tagttttgtc tccttagccg
     1021 gcggcgcagg ttctgctgcg agccggattg aagtctgctc ccagatcacg gtacggcgga
     1081 agggtggaga aagttccgtt gcagccaact ctgccaacgg cttatgcttc caaccagagg
     1141 aaattaagct tggggtcttc cgggtcgctc ttcgggagac gaggtgaaat tgctacttcg
     1201 atttaggctg cgctccttac gggccgtagc cgtctactct ttgctgatcg ttgccgtcca
     1261 ggttctgcgc gcgcaggaaa ccaaggaaaa agttacggtc gacgatgtcg agcgcagcta
     1321 cacgctgcga ttgcccagcg gctacgatgc ccagcgccgc tatccggtgg tgattctgct
     1381 gcatgggatg aaccaggatg ccgacgacat ggagcggctg acgcaatttg acgcgctggc
     1441 cgacaaggac ggcatagtcg ctgtctatcc cttcgccttg cacgggcgct ggaataccgg
     1501 agtgcatccg caggagcggc ggcccatggg catggggccc ggtggcgggc gccatcgcgg
     1561 cggctacccg ggagggggtg gttatcccgg cggaggtggc ggctacccgg gcggcggcca
     1621 acccagaagt cgagagccgg acgaggagcg aaggccggct ccggcggacg acatcgcgtt
     1681 cttcaatcgg atgctggacc agctccaatc caaattgtcg attgatgcat cgcgaatcta
     1741 cgttgccggg ctctcggaag gcgggttcat gagcatgcgc gcggcttgcg ccatgtcgga
     1801 ccgcgttgcc gcgattgcgg ctgtcggagc agccatgccg aagaccatga tctgcctgcc
     1861 ctcgcgcccg gtgccggtgg ccatgatcaa tggaacatcc gatcccgtcg ttccttatgg
     1921 cggcggcact gagcacaatc tgaatctggc gactctttcc gcggaggaca gcgccaaagc
     1981 ctgggccaag atcgatcgct gtgaggaaaa accggctcac gacaagcttc cgtccaggga
     2041 aaaaggcggg atggagacga aagtcgacac ctacaccgct tgtcagcagg gcgcacaggt
     2101 cgtgctctac agcgtgaagg gagctggaaa tacctggccc ggaggggaac aatacgaggc
     2161 cgagaacacc gtcggcaaga ccagccacga tttgaatgcc aatgagacgc tgtggagctt
     2221 cctggtcacg aagaaaatgg ctgcgaagaa cgacagcggg aagtaacagg tacttcgccc
     2281 tccgattgtc cagtttgaaa agtgaatcgc ggagaggcag ggctctccgc gattctgtaa
     2341 cgacctggcc cgaattcagc cgcgccagct cggttcaccg gttatttctt cacttcattc
     2401 gcggacttca ctcctacaac gagcgcagaa agttcagaag atcctgcatc tgcgacggat
     2461 tcagagtgcg gaacctgcga actacgcggt tggcttccga gtcccggtcg ccgtgagcgt
     2521 tgatcgcttc gatcaggtcg ctggtacggc catcgtgcag gaagtacagc cgttggccca
     2581 gcccccaaag cggagcggtg cggaattcgt ccgccccggc gttcccctga tggatgccat
     2641 ccgccaggtt ccagcccatg tgatgcaaag cgaagtccga atacgggtgg tatgtgatgt
     2701 tcgacatacc ggtgtagtcc gacggccccg cagtcagcga gggcgagtga caaagagcac
     2761 agccgaccga attgaacagc gcggctccgt tctgggttga cgttgtcggc ggcgccggtg
     2821 tgggaggcgc cgacatgcgg ctgaacgcgg cgaagttcac gacatccgac gacatttcag
     2881 agaccgtccc gacctgggtg ccatagttcg ggctgctcgg attcgggttg agcatgttcg
     2941 aggcatcctc cggggaattg ttgaacacgc aaccagccac cgcgctgcgc tcgttgggaa
     3001 acaactcgtt cgaaacgccc tgctcaacgt tgtaggcttc tccaacgaac accagcagcg
     3061 atttgttctg ggccttccag ccaaaacggg taatggtgcc gtcattaccg ctggtattga
     3121 acgtgccccc gatcctcatg gcggctcgtg ctgcctgagt ggacgcaaga ttggcttgca
     3181 gtatcgagtc cggagtagct tccactaggc ccaggccgaa cagtggtgtg gggatgcgga
     3241 agatcacgtt attgttggca agttgggtgg caaagtcggg ctgcccgagc atgcaaccca
     3301 ccgcatcgga ccggccggcg atcgtgtaga gatcgtgcac gcccccatcg cgcgtgccgt
     3361 caggattcct gatgaaacgg gcttcgcgca cgggcccgtt ggcggtgata aaactgggaa
     3421 cggtgttgcc agcgctgtcc aggatagcca gcgcgacctg gggattctgg atggagtgct
     3481 gcgggctggc cagcccaggg ctgctgccgc cgactgccgg ctgcgcgtgg cacatggcgc
     3541 agctattgcc attgaaggtc ggacccagac ccgatcctgt ttcgccagcg atgccgcccg
     3601 agaccgagtc gacttcctgg aaacgttgtt gtgcctgacc gaagaaagtc tgctcattcg
     3661 cattcaagcc ggggaaaaag ctgcctgcgg ccgctggtcc gggacgaggg ccggggtcgt
     3721 tttgcgactg aacttccggg ccgcacagca aagtcgcggc caggccaaca acaacaagcg
     3781 ggacatagcg atacctcatt ttgcacctcc gtgcaggggt agacgtactg cggccttaat
     3841 ttcgtggtgc gtttggcgaa ggcgggcgca gggggagttc tgctgttcag ttcaagctgc
     3901 gggccagtcc gagcgctggt gccgcgcccc accgctaaca gaacagcgct aggggagtgg
     3961 tgggaacccc gtctgaatgc tctccccgga tggggaacga gcggttacta cattgcgtga
     4021 gagtgtaggc tgggcgcggg ttttggtcaa gtgcacacaa gtgcatcgag aaaaactggt
     4081 ctgatttctg gagtcgttat tctctaaccg cgggcagtcg tgaaccttag gccattggcc
     4141 aatttcacgc gatgcacttt ctcagttcgc ggcgcaggat ctttcccgag gcggttttgg
     4201 gaaccacctc gacaaagcgc acttcccgcg gctgcttgta gtgcgtgaga cgatctgcga
     4261 caaatgcgca aagttcctcg ctcagcttgt gactgtccac aaacccatcg cgtagcgcga
     4321 cgaaggcgac cgggacttct cccgccgctg catcgggacg gccgacgacc ccgcactctt
     4381 tcactgccgg gtgctcgagc agcacggctt ccacttcggc gggagccacc gcgaagccct
     4441 tatatttgat catttccttg cggcggtcga ccacgcgata gaagccttcc ttgtcacggg
     4501 tgacgatatc gcccgaccag taccagccgt cgcgcaagac agcggcggtg gcttgcggat
     4561 ctttccagta gcccagcatg aactgcgggc cgcgcatcac cagctcgccg gggcagcccg
     4621 aaggaacttc cgtgatgctc tcgggcgtat cggagggatc gatctccgct tgcgccagaa
     4681 cgcgacaatc ggtctgagcc agcggctgcc cgatgctctc gggatggtag agcgcaggat
     4741 cgaggaagcc gacgtgtgtc acggg
//
LOCUS       DASPUL010000590         3391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1336904, whole genome shotgun sequence.
ACCESSION   DASPUL010000590 DASPUL010000000
VERSION     DASPUL010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336904"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..693
                     /locus_tag="VEJ00_13735"
     CDS             112..693
                     /locus_tag="VEJ00_13735"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HXX02271.1"
                     /translation="MKSSLPLLLLVALVSSYAVAASVTLNTTSLPNGTVKTAYSAVIN
                     ASGGCTPYQWAIVSGNLPAGIARKTSSNTTVLDLSGIPTTAASYSFTVQVTGCGGRTS
                     RASYKIVIQSTANHVVDLDWKASTSKNVAGYNVYRTTAGSTWKKLNVSLIASTLYSDS
                     TVANNTTYLYSATTVDIYGHESSKSAAVQVAVP"
     gene            912..3227
                     /locus_tag="VEJ00_13740"
     CDS             912..3227
                     /locus_tag="VEJ00_13740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX02272.1"
                     /translation="MSRQVALRLLCLCCLLAVFAGCTRDPNVRKQKYFASGQRFFAQG
                     RYREAVIQFSNAIQIDSRFADAHYQLAQTYLKLSDSNRAYPELARTVELAPDNYRAHT
                     DLANLLVTVRNPDGTPIQESLNLAKTHLDLLREKVPNAPETHEAWANYYAAQNNLGQA
                     LQEMQQAIGLDPNRSESYLLLALLELRDNQADQAEANFRKAVEVDPKSMNAQLALGGF
                     YQARNRLPEAEQQFRHAIDVDRKNPAPRSALVRLLMAEGKTAETEALLQQTKKEISDN
                     SDGYRMLGDFYFHNGELDKAVEEYTSLYKEHPKDILVKKNYIQLLILKNRLDDAARLD
                     DEVLKANSRDVDALVYRGQIMLRRNQNSAAVEALQSALHDDPDNAVGHFQLGVAYDQQ
                     HDDARAQSEWREAVRIRPDLTDAQRALAALEIRRGDIDALLQTAQQIITAQPYAPDGY
                     LLKGVADLARQNFPDAQHDAEQAAQRAPQSAAPYIQMGNIQLIQKHHAEASKYYQQAL
                     DRDPSSADGLSGLMNTYFAQKQYDQAVAAAEVQITKSPDNSNFYDLLGTALFNGKKDL
                     PGAETALRKAVDLDKNNTDALEKLGKVQIQEGNADRAIALYQQSIKDNPREVRFYVLS
                     GELYESKRDWDHAKQMYQQALTIQPEHALASNNLAYVMLEQGGNVDVAMAMAQTARRR
                     MPDWPNAADTLGWAYYQKGIYQSAISQFQEALRLTEKHGAPDDATIHYHLGMAYQKSN
                     QNALARQQLEKAVKLSPNNADAKKALADLRG"
BASE COUNT          759 a         1160 c          903 g          569 t
ORIGIN      
        1 cccgcgctga agcttctgtc ggaactaagc caaacagatt agaaccagcg ggaagtctgc
       61 aaacccgtgg aatctgtccc aaatcggtac tcgccgggca ggatggagct catgaaatcc
      121 agcctcccct tgttgttgct cgttgcactc gtgtcctcgt acgccgtcgc ggccagcgtc
      181 accctcaaca ccaccagctt gccgaacgga accgtcaaga cggcatactc cgccgtgatc
      241 aacgccagcg gcggctgcac tccctaccag tgggccatcg tctcgggaaa tctcccggcc
      301 ggcatcgcca ggaagacttc cagcaatacc actgtgctcg acctcagcgg aattcccacg
      361 acggcggctt cctattcgtt caccgtccag gtcaccggct gtggcgggcg tacttcgagg
      421 gcgtcctaca agattgtgat ccagtcgacc gcaaaccacg tcgtggacct ggattggaag
      481 gcttcgacat cgaagaacgt ggctggctac aacgtctatc gcactaccgc cggatcgacc
      541 tggaaaaagc tcaacgtgag cttgatcgct tcgacgctct acagcgactc caccgtcgcc
      601 aacaatacga cctacttgta ttcggcaact accgtcgaca tctacggcca cgagagcagc
      661 aagtccgccg cggtccaggt agcggtcccg taactgcggc ggccttctgc gtcggccaga
      721 ttccccacag gcatcacgcc cgctccgccg cagaaggctt cgcccaagac gacgccctcg
      781 aaaactcgcc gaagttcaaa ctgaccatta cctcactgcc gactgtctcc cgcgccctgc
      841 cctgaagtat catgaaacag cgcgcggaca gcctttccta caggcttgcg cgcgaatcgg
      901 gagaccctgc tatgagtcgt caagtcgctc tccgcttgct ctgcctgtgt tgtctgctgg
      961 ccgtgttcgc cggctgcacc cgcgatccta acgtgcgcaa gcagaagtat ttcgccagcg
     1021 gccagcgctt cttcgcacag ggcagatatc gcgaagcggt gatccagttc tccaatgcca
     1081 ttcagatcga ctcccgcttc gccgacgccc actatcagct ggcgcagacc tacctgaagc
     1141 tgagcgacag caaccgcgct tatcccgaac tggcccgcac cgtcgagctc gctcccgaca
     1201 actatcgcgc ccacaccgat ctcgccaacc tgctggtcac ggtgcgcaat cccgacggca
     1261 cgcctattca ggaatcgctg aacctggcca agactcatct cgacctcctc cgcgagaagg
     1321 tgcccaacgc tccggaaacc cacgaagcct gggcgaacta ctacgctgcg caaaacaatc
     1381 tcggccaggc cttgcaggag atgcagcagg cgatcgggct cgatcccaac cgctccgagt
     1441 cttatcttct ccttgcgctc ctcgagctcc gcgacaacca ggcggatcag gcggaagcca
     1501 atttccggaa ggcggttgaa gtcgatccca aatcaatgaa cgcacagctg gcgctgggcg
     1561 gcttctatca ggcgcgcaac cggctgcccg aggccgaaca gcagttccgc cacgccatcg
     1621 acgttgatcg caagaacccg gcgccccgct ccgccctggt ccgtctgctc atggcggaag
     1681 gcaaaaccgc agagaccgaa gcccttctgc aacaaaccaa gaaggaaatc tccgacaact
     1741 ccgacggcta ccgcatgctg ggcgatttct actttcacaa cggcgaactc gacaaggccg
     1801 tcgaagagta cacctcgctg tacaaagaac atcccaaaga catcctggtc aagaagaact
     1861 acatccagct attgattctg aagaaccgtc tcgacgatgc cgccagactc gacgacgagg
     1921 ttttgaaggc caattcgcgc gatgtcgacg cgctcgtcta ccgcggccag atcatgctgc
     1981 ggcgcaatca gaacagcgct gccgtcgaag ccttgcagag tgctttgcac gacgaccccg
     2041 acaatgccgt cggccacttc cagcttggcg tcgcttacga tcagcagcac gatgacgccc
     2101 gcgcccaatc ggaatggcgc gaggcggttc gcatccggcc cgatctcacc gatgcccagc
     2161 gcgccctggc cgcgcttgaa atccggcggg gtgatatcga cgcgctgctt cagaccgccc
     2221 agcagatcat caccgcgcag ccctacgcgc ccgacggcta cctgctcaaa ggcgtggccg
     2281 atctcgcccg gcagaacttt cccgatgcgc agcacgatgc cgagcaggcc gcgcagcgcg
     2341 ctccgcagag cgcggcgcct tacatccaga tgggcaacat ccagctcatc cagaaacatc
     2401 acgccgaagc ctccaagtac tatcagcagg cgctggaccg cgacccctcc tcggctgacg
     2461 gactcagcgg cctgatgaac acctacttcg cgcagaagca gtacgatcag gccgtggccg
     2521 cggcggaggt gcaaatcacc aagtcgcccg acaacagtaa tttctacgat ctgttaggca
     2581 ccgcgctctt caacggcaag aaagatttgc cgggcgccga aaccgcgctg cgcaaggccg
     2641 tcgatctcga caagaacaat accgacgcgc tcgaaaagct gggcaaggtg caaattcagg
     2701 agggcaacgc cgaccgggcg atcgccctat atcagcagtc gatcaaggac aatccccgcg
     2761 aagttcgctt ctacgttctc tccggcgagc tctacgaatc gaagcgcgac tgggatcacg
     2821 ccaagcagat gtatcagcag gcgctgacca tccagcccga gcatgcgctg gcctccaaca
     2881 acctcgctta cgtgatgctc gagcagggcg gcaacgtcga cgtggccatg gcgatggccc
     2941 agaccgcccg tcgccgcatg ccagactggc ccaacgccgc cgacaccctg ggctgggcct
     3001 actatcagaa gggaatttat cagtcggcca tcagccagtt tcaggaagcg ctccgcctga
     3061 ccgagaaaca tggcgcgccc gatgacgcga caatccacta ccacctggga atggcgtatc
     3121 agaagtcgaa ccagaacgcc ctcgcccgcc agcaactgga aaaagcagta aagctcagcc
     3181 ccaacaacgc cgacgccaaa aaagcgctgg ccgacctgcg gggatagaaa cttcgcggat
     3241 agaaaatacg cccgaagcca cagatttttc tattcctcgg ctctggcaaa tctgcactgg
     3301 catatacatc gccctggaag agcggccctt cagggccgcg tcgccggagc tcaaagagat
     3361 tgggctttag cccaggtgtc ctgcgacgaa c
//
LOCUS       DASPUL010000591         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_662483, whole genome shotgun sequence.
ACCESSION   DASPUL010000591 DASPUL010000000
VERSION     DASPUL010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662483"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VEJ00_13745"
     CDS             <1..778
                     /locus_tag="VEJ00_13745"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HXX02273.1"
                     /translation="SALDAARVVFARFTPGAIETEFKAGHDPVTEADRAVDAVLRRNL
                     LRDGEGWLSEESVDDVSRLNKSRVWVVDPLDGTREFVAGIPEFCVSIGFVENARPVAG
                     GICNPATNETIIGAIDCGVLYNGQPAHPSQRTSLKGALILASRSEVKRGEWQQFQGGE
                     FQIRPMGSVAYKLALVAAGRADVTFTLTPKNEWDVAAGAALVESAGGFVATLDNAPLR
                     CNNRNPLLSGLLASGPLLREPLVASLARHLPTATAVHSRS"
     gene            complement(926..1981)
                     /locus_tag="VEJ00_13750"
     CDS             complement(926..1981)
                     /locus_tag="VEJ00_13750"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXX02274.1"
                     /translation="MIKRQLRRAALTTLKGGGAFGLVRDSAWRRQRLLILCFHGVALD
                     DEDQWRPSLYISPQQLERRLEILRRERCAVLPMGEALERLYRHDLPPCSVALTFDDGQ
                     YDFYRQAYPRLKQSGFPATVYLTTYYSELQRPIFSLVCSYLLWKARHAGAADLREFGI
                     QGAARLDSQQSREAAAGQIVQWADGQDLHGQQKDQIAASLAQRLGIDYDALRARRILH
                     VMNPQEVRELAAAGVDFQLHTHRHRTPMSEELFRQEIRENRRRLASIIGGGQTHFCYP
                     SGAYRPEFLPWLAAEGIVSAVTCDTGFATAETNPLLLPRLVDTSARTDLEFESWVTGV
                     GHFLSRRRRAKLAYSSD"
     gene            complement(1978..>2694)
                     /locus_tag="VEJ00_13755"
     CDS             complement(1978..>2694)
                     /locus_tag="VEJ00_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HXX02275.1"
                     /translation="KLGHTFLVRDGNSWPSFYYDNFFSAFSAGEQAELLTPDALATAG
                     DAYAGSMGAWNRSQGDLLHRLLYADINSYLIELLMKQDQMSMAASIESRVPFLDHVLV
                     EFTAKIPERYSIAGMAGKFVLKQAVEDLLPREIIYRKKMGFPTPWDFWLAGSKLDELE
                     RTLLEPRSMERGIFRPEVVKGIFAEHRAKARDHGNRIWRLLNFELWMRVMIDGEPAED
                     VAAHSHSQLAAQGMGGRKTP"
BASE COUNT          524 a          886 c          769 g          515 t
ORIGIN      
        1 gtccgctctg gacgccgccc gcgtcgtctt cgcgcgcttc accccgggcg cgattgaaac
       61 cgaattcaaa gcaggacatg accccgtgac cgaggccgat cgcgccgtcg acgccgtgct
      121 gcgcaggaac ttgctgcgcg acggcgaagg ctggctttcc gaagaaagcg tcgacgacgt
      181 ttcccggctg aacaagagcc gcgtctgggt cgtggatccg ctcgatggca ctcgtgaatt
      241 tgtggccgga attcccgaat tctgcgtttc tatcggattc gtagaaaacg cacggccggt
      301 cgccggcggc atttgcaatc ccgcaaccaa cgaaaccatc atcggcgcca tcgattgtgg
      361 cgtgctctac aacggccagc ctgcccaccc cagccagcgg acgtcgctca aaggagcgct
      421 gattctcgcc agccgcagcg aagtaaaacg cggcgaatgg caacaatttc agggcggaga
      481 gtttcagatt cgccccatgg ggtcggtggc atacaagctg gcgctggtgg ccgccggccg
      541 cgcggatgtc accttcacgc tcactcccaa gaacgagtgg gacgtcgccg cgggcgcggc
      601 cctggtcgaa agcgcgggag gcttcgtcgc tacgctcgac aacgctcctc tgcgctgcaa
      661 caaccgcaat cctctgctct cgggcctgct cgccagcggt ccactgctgc gcgagccgct
      721 ggtggcttcg cttgcacgcc acctcccgac ggctacggca gtacattccc gctcctgaac
      781 tcctcaactt gtggattcta gaagctctcg caaatcggtt tgaaagggtg cggcttccag
      841 cctcgccgct cggcacaaga aagaccgggc tttagcccct gaggggaaga gactcttact
      901 ttcttggctc agtatgagat agcttctagt cagatgagta cgcgagcttt gctcgcctgc
      961 gtcgtgaaag aaagtggcca acgccggtca cccagctttc aaactccaga tccgtccgcg
     1021 ccgacgtgtc caccagacgt ggaagcaaca gaggattagt ctcggccgtc gcgaaccccg
     1081 tgtcgcacgt tacagccgac acgattccct ccgcagccag ccagggcaga aactcaggcc
     1141 ggtaggcgcc gctgggatag cagaagtgcg tctgcccgcc accgattatg ctcgccagcc
     1201 ggcggcggtt ctcgcgaatc tcctgccgga acagttcctc actcatcggc gtgcgatgcc
     1261 ggtgggtgtg tagctgaaaa tccacgcctg cggcggccag ttctctcact tcctgcggat
     1321 tcatcacatg cagaatccgc cgggcgcgaa gcgcgtcata gtcgatcccc agcctctggg
     1381 cgagactcgc cgcgatctgg tctttttgct gtccgtgaag atcctgtccg tccgcccact
     1441 gcacgatctg gccggctgcc gcttcccggc tttgctgcga atcgagcctc gcggcaccct
     1501 gaattccgaa ctcccttaaa tcagccgcac cagcgtgcct cgccttccac aacaggtaag
     1561 aacaaaccag gctgaaaatg ggccgttgca gctcgctgta gtaagtcgtg agatatacgg
     1621 tcgcggggaa cccgctctgc ttcagcctgg gatatgcctg ccggtagaaa tcgtactggc
     1681 cgtcatcaaa ggtcagcgca acgctacacg gcggaagatc gtgtctgtag agccgctcca
     1741 gggcttcccc catcggcaac acagcgcacc tctcgcggcg caaaatctcc agccgccgct
     1801 ccagttgttg cgggctgatg tagagggaag gccgccattg gtcttcatcg tcgagagcta
     1861 cgccgtggaa gcagaggatc aggagccgct gccgccgcca ggcactatcc cgaaccagtc
     1921 cgaacgcgcc tccgcctttg agcgttgtca gcgccgcacg ccgaagttgc cgtttgatca
     1981 aggcgtcttc cggcctccca tgccctgagc ggcaagttgg gaatggctgt gtgccgcgac
     2041 gtcctccgcc ggctcgccgt caatcatgac tcgcatccag agctcaaaat tcaggagccg
     2101 ccagatccgg tttccatggt cgcgcgcttt cgcgcgatgc tcggcgaaga ttcccttcac
     2161 gacttccggc cggaatatgc cccgctccat gctgcgcggc tcgagcaggg tgcgctcgag
     2221 ctcatcgagc tttgatcctg ccaaccagaa atcccacggc gtaggaaatc ccatcttctt
     2281 gcgataaata atctctcgtg gcaacagatc ttcgaccgcc tgcttcagga cgaacttgcc
     2341 cgccatgccc gcgatcgaat agcgctctgg aattttcgcg gtgaactcga ccagaacatg
     2401 gtcgaggaac ggtacccggc tctcgatcga ggccgccatg ctcatctggt cctgcttcat
     2461 gagcagctca atcagatagc tgttaatgtc tgcatacagc aagcgatgca gcagatcgcc
     2521 ctgcgaccgg ttccacgcgc ccatcgagcc cgcgtaggca tcgcccgcag tcgccagtgc
     2581 gtcaggagta agcaattccg cctgctcccc cgcagagaag gcggagaaga aattgtcgta
     2641 gtagaaagac ggccacgaat tcccgtcgcg caccaggaac gtatgcccca gctt
//
LOCUS       DASPUL010000592         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1065757, whole genome shotgun sequence.
ACCESSION   DASPUL010000592 DASPUL010000000
VERSION     DASPUL010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065757"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VEJ00_13760"
     CDS             complement(<1..307)
                     /locus_tag="VEJ00_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02276.1"
                     /translation="MSTPQLLLERYVEAKDLTRPQLMPDIYAPDAILTFSIATDSIDF
                     PRRVAGRDAITRTLVVDFAARFSRCKTFYVCESPPRETAHPVVLPWLVLMREGALDQL
                     "
     gene            complement(328..741)
                     /locus_tag="VEJ00_13765"
     CDS             complement(328..741)
                     /locus_tag="VEJ00_13765"
                     /inference="COORDINATES: protein motif:HMM:NF024307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HXX02277.1"
                     /translation="MSQVRITVGTLRFVARFEEVAAPRTCAAFRALLPFEKPLIQARW
                     SGEAAWVPLGDLKLDVPMENATSHPSRGDLLFYPGGASETELLLPYGSTRFAAKVGQL
                     AGNHFLTIEIGLENLAEVGRLVLWQGAQTILIELT"
     gene            843..1718
                     /locus_tag="VEJ00_13770"
     CDS             843..1718
                     /locus_tag="VEJ00_13770"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HXX02278.1"
                     /translation="MAVEFVHPEFLVSTDWLAQQLDNPAVCVVDCTTHTLPWPDFSLY
                     DVVSGRADFEKAHVPGAIFVDLDHDVSAPHPRLHFTLPSPERFADAMGRLGIGDETHV
                     VAYSTANHWWASRLWWMLRVFGHDRASLLDGGFPTWQREGRQVENGPARPRSVAHFTP
                     RYRPEMLATKEDVLAAIGQKETCTVNALRREQHAGSGGVYYGRRGRITGSINLPAAEH
                     VRADNTFKSAQELRPMFSSALAQPQVITYCGGGIAATSVALVLAMLGYDRVKVYDNSL
                     TEWAADPSLPMEIGA"
     gene            complement(1791..3326)
                     /locus_tag="VEJ00_13775"
     CDS             complement(1791..3326)
                     /locus_tag="VEJ00_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HXX02279.1"
                     /translation="MSNNATTINALLEAGAPDATALCAPGGVPLSYRALRALASQTTS
                     ALNAQGVGRNDRVAIVLDNGPEMAAAFLCVASGATAAPLNPAYRSEEFEFYLTDLNAK
                     ILVIERDKSSPAVEVAHKLGIPIVRLRATPEKGAGSFILEFPPGLTLPKAGRAGPATP
                     DDVALVLHTSGTTSRPKIVPLAQRNVCASARHIRETLAFSKADRGLNIMPLFHIHGLI
                     AGTLAPLSAGGEICCTTGFNALRFFAWIAEVRPTWYTAVPTMHQAILSRAAKSAEVIK
                     ANPLRFIRSSSSSLAPQIIKELEEVFGAPVIESYGMTEAAHQMASNPLPPGKRKPGTV
                     GIAAGPEIGVIDADGNLLACGATGEIAIRGPNVMSGYENNPKANGEAFVNGWFRTGDQ
                     GVMDAEGYITITGRLKEIINRGGEKISPREVDEVLLDHPAVQQCVTFALPHDKLGEEV
                     AAAVVLREGAQAGEKDLREFAARRLADFKVPRKILILPEIPKGATGKTQRIGLAQKLG
                     LTQ"
BASE COUNT          629 a         1086 c         1139 g          628 t
ORIGIN      
        1 gcagctgatc caaagcgccc tcacgcatga gaaccagcca cggaaggacg acgggatggg
       61 cggtttcccg cggcggcgac tcgcagacgt agaacgtttt gcagcggctg aaccgggcgg
      121 cgaaatcgac gaccagcgtc cgggtgatcg catcgcggcc cgccacccgg cgagggaaat
      181 cgatcgagtc ggtagcaatc gaaaacgtga gtatcgcgtc gggcgcgtag atgtccggca
      241 tgagctgggg ccgcgtcaag tctttcgctt cgacgtagcg ctcgagcagc agttgcggag
      301 tcgacatcgc ggtgtccggc gaagacctta ggtgagctcg atcaggatcg tctgagctcc
      361 ctgccacaat accagccggc cgacctcggc aagattctcc aggccgattt cgatggtcag
      421 aaagtggttg cctgcgagtt ggccgacttt ggcggcgaag cgcgtgctgc catagggcag
      481 cagcaactcc gtttcgctcg cgcccccggg atagaacaga agatctccgc gtgagggatg
      541 actggtggca ttctccatcg gcacatcgag tttgagatcg ccgagcggca cccatgcagc
      601 ctctccgctc cagcgcgcct ggatcagcgg tttctcgaat ggcagcaagg cgcgaaacgc
      661 ggcgcaggtg cgtggagcgg ccacttcctc gaagcgtgcc acgaatcgca gcgtgccgac
      721 cgtaatcctg acttgagaca tcgagttatc gtactcccag ctccagagcg cggacagtgc
      781 ggccgccgca aagcggccat tgtaagattg atggcctcac agtctcgaaa ggcgggtctc
      841 ccatggcggt cgaatttgtt cacccggaat tcctggtcag cacggactgg ctagcgcaac
      901 agctggacaa tccagccgtg tgtgtcgtcg actgcaccac ccacacgcta ccctggcccg
      961 atttctcgct ctacgacgtg gtgtccggcc gagcggactt cgagaaggcc catgttccgg
     1021 gcgcgatctt cgtggacctc gatcacgacg tgtccgcgcc gcacccgcgc ttgcacttca
     1081 cgctgccatc gcccgaacgg ttcgcagacg cgatggggcg gttgggaatc ggcgacgaaa
     1141 cacacgtggt tgcctattcg accgcgaatc actggtgggc gagtcggttg tggtggatgc
     1201 tgcgcgtgtt cggccacgac cgtgccagcc ttctagacgg cggctttccg acatggcagc
     1261 gcgaaggccg gcaggtggag aacggtcccg cgcgtccgag aagcgtcgcg cactttaccc
     1321 cgcgctaccg gccggagatg ctcgcgacga aggaggacgt cctcgcggcg atcggacaga
     1381 aggaaacctg cacagtgaat gcgctgcgca gggaacagca tgccggcagc ggcggggtct
     1441 actacggccg gcgcggccgc attaccggca gcatcaacct tcccgccgcc gagcacgtca
     1501 gagcggacaa taccttcaag agcgcgcagg agctgaggcc gatgttctcg tcggcgctgg
     1561 ctcagccgca agtgattacc tattgcgggg gaggtattgc agcgaccagt gtggcgctcg
     1621 tgctggcgat gctcggctac gatcgcgtca aggtgtacga caactcgttg accgaatggg
     1681 cagccgaccc gtcgctaccg atggagatcg gcgcgtagac gctgctccag gtgatcaggt
     1741 caccgattga ggcgaactga acggcgcgcc ggccggcgcg tgctgactgg ctactgcgtc
     1801 aatccgagtt tctgcgccag gccgatgcgc tgagtcttgc ccgtggctcc cttggggatc
     1861 tcgggcagga tcaggatctt gcgcgggacc ttgaagtccg ccaggcgcct ggcggcgaat
     1921 tcgcgcaggt ccttctcgcc ggcttgagcg ccttcgcgca gcactaccgc cgctgccacc
     1981 tcctcgccga gcttgtcgtg cgggagcgcg aaggtcacgc attgctggac cgcgggatgg
     2041 tcgagcagga cttcatcgac ctcgcgcggc gaaattttct cgccgccgcg attgatgatc
     2101 tccttcaacc gtccggtgat tgtgatgtag ccctccgcat ccatcacacc ctggtcgccg
     2161 gtgcgaaacc agccgttgac gaaagcctcg ccgttggcct tggggttgtt ctcatagccg
     2221 gacatgacgt tcggcccccg gatcgcaatt tctcccgtag cgccgcaagc caacaggttt
     2281 ccgtcggcat cgatcacgcc gatctcgggg cctgccgcga tccccaccgt tccgggcttg
     2341 cgtttccccg gcggcagcgg gttgctggcc atctgatgtg cagcttccgt catgccatag
     2401 gactcgatca caggtgcccc gaacacctcc tccagctctt tgatgatctg cggcgcgaga
     2461 gaagacgaag acgaacgaat gaagcgcagc gggttggctt tgatcacctc cgcgctcttc
     2521 gccgcacgcg agagaatggc ctggtgcatg gtggggacgg ccgtgtacca ggtgggccga
     2581 acctctgcta tccacgcgaa aaagcgcaag gcattgaacc ccgtagtgca gcagatctcg
     2641 ccgcccgccg acagcggcgc cagtgttccg gcgatcaagc catgaatgtg gaacagcggc
     2701 atgatgttga ggccgcggtc cgctttcgag aacgccagcg tctcgcggat atgacgggca
     2761 gacgcgcata cgttgcgctg ggccagcgga acgattttgg gccgcgaagt cgtgcccgag
     2821 gtgtgcagca cgagcgcgac atcgtcgggc gttgcggggc cagcgcggcc cgccttgggc
     2881 agcgtaagtc ccggcgggaa ttccagaatg aagctgccag cgcctttttc cggagtagcc
     2941 ctgaggcgga cgatgggaat gccgagcttg tgcgctacct caaccgcggg cgacgacttg
     3001 tcgcgctcga tgacgagaat cttggcgttg aggtcggtca gatagaactc gaactcttcg
     3061 ctccgatagg cgggattcag gggtgccgcg gtggcgccgg acgcgacgca caggaacgcg
     3121 gccgccatct ctgggccgtt gtcgagcaca atcgcgacgc ggtcgttgcg gccgacacct
     3181 tgcgcgttga gagcagacgt cgtctggctc gcaagcgcac gcagcgcccg atagctgaga
     3241 ggcacgccgc cgggcgcgca cagcgcggtt gcatccggtg cgccggcttc cagcagtgcg
     3301 ttgatcgtcg tggcgttgtt gctcatttcg gaccttcctt tagcaggtaa caagcgcgac
     3361 aaccaccccg cccactgtgt gggcacccct cctccaagag gacgggaaaa atctgttttc
     3421 tcccctcctt ttcaaggagg ggtggcgcga agcgacgggg tggttagaca tgcgcgcaac
     3481 ga
//
LOCUS       DASPUL010000593         2908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2033945, whole genome shotgun sequence.
ACCESSION   DASPUL010000593 DASPUL010000000
VERSION     DASPUL010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2033945"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VEJ00_13780"
     CDS             <1..245
                     /locus_tag="VEJ00_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02280.1"
                     /translation="PNFGNPLMPNFSVNLENNGTVTPLSFTGPCSTAPGTDHTGCRAV
                     GPGFLPIAGTPDVVAGEPFIGGGGPRNIQLAVKFTF"
     gene            452..1927
                     /locus_tag="VEJ00_13785"
     CDS             452..1927
                     /locus_tag="VEJ00_13785"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX02281.1"
                     /translation="MRRSNCFQAAVLILGLCLCVGMAAPAFAHHLKKGETTSLPITTS
                     SAKARQLYRKGMADYENLYLERCNDDWRAAVKEDPNLAVAWAWIAFNSSNPQEVSAAR
                     SKAKELAPKLTPGEQLMIAWISKVQEGDYLGGITAMNDMLEMYPRDKHLLYLAGNWLM
                     AENGNDQAERYMEKALALDKNFPAALNDLAYVDARNRKFNKAFAAMDRYVALLPSEPN
                     PEDSYGELKRMAGDFQSSLEHYRAALKIDPDFVTSQLGLGDTYALMGNQEQARAEYDK
                     AIRYAHNEADRLTYGMQKAMTYVRDGNFAEADKEYQEIAETAHAKEQDLQEAQAFGRM
                     AEYQSDDNAALKDLKLAEEALGHRATISPADRDEEMSRILRNRAVRAARAGNQVLADK
                     SQQQLEAMASGSRNRVIQASYNGAAGTLLMDQKKYEDAIAHLEEDQDNPFTMELLVQA
                     YYETGATEKLHEMEAKLRGTNVPTMEQALVVPAARAKRPNI"
     gene            2109..2426
                     /locus_tag="VEJ00_13790"
     CDS             2109..2426
                     /locus_tag="VEJ00_13790"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXX02282.1"
                     /translation="MAHSERRSGTRISTRVATRVRTSQGADHTAQTRDVSANGVFLYT
                     KSRMEKGSEVELVLILPPELTSGEKYWVCCQATIVRVEEEGSEFGVAAQIRRMDILPE
                     VAV"
     gene            2639..>2908
                     /locus_tag="VEJ00_13795"
     CDS             2639..>2908
                     /locus_tag="VEJ00_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02283.1"
                     /translation="MLIDKLAAGVVQVQTPIGPRYVMPSFKQRVYLLWVFRNFPILPH
                     AVLSRRQQRMIDRMCSEQRFASMAYADGMNEAPVIGTVEHRPAISP"
BASE COUNT          607 a          862 c          926 g          513 t
ORIGIN      
        1 acccgaattt tgggaatccc cttatgccca acttctccgt gaacctggag aacaatggca
       61 cggtgacccc actaagcttt acgggtccgt gcagcaccgc tcccgggaca gatcacaccg
      121 gatgccgcgc ggtcggtcca ggcttcctcc cgatcgcggg cacgccggac gtggtagctg
      181 gtgagccgtt catcggaggt ggaggaccac ggaacatcca attggcggtc aagttcactt
      241 tctaattccc ggcacttgcg agcagcgccc ctgccaactt acggcggggg tttttgtttc
      301 cggcaaccga ggcgaggcga gcggggcctg gtctacacgg ttcgcatgcg cactcggcca
      361 ctgcctttcg tcacctcgtg cctggattac tgcgatgtta cgctgtgcgg gtaagcctcc
      421 ccataaagct ctgttccgga gaaaatcccg catgcgtcgc tccaattgct ttcaggctgc
      481 agtcctgatt cttggtctct gtctttgtgt aggcatggct gcgccggctt tcgcgcacca
      541 tctcaagaaa ggtgagacga cctcgctgcc gatcacgacc tcgtcggcca aggcgcgcca
      601 gctctatcgg aagggcatgg ccgactacga gaatctctac ctggagcgct gcaacgacga
      661 ctggcgggcg gcggtgaaag aagatccgaa tcttgctgta gcctgggcct ggattgcgtt
      721 caacagcagc aatccgcagg aagtcagtgc ggcccgctcc aaggccaagg aactggcgcc
      781 caagctgacg ccgggcgagc agttgatgat cgcctggatt tcgaaggtgc aggaaggcga
      841 ctacctgggc ggcatcacgg cgatgaacga catgctggag atgtatccgc gcgacaagca
      901 cctgttgtat ctggccggga actggctgat ggcagagaac ggaaacgacc aggccgagcg
      961 ctatatggag aaagcgctag cgctggacaa gaatttcccc gcggcgctca acgatctggc
     1021 ctatgtcgac gcccgcaacc gcaagtttaa caaggcgttc gccgccatgg accgctatgt
     1081 cgcgctgctg ccgagcgagc ccaatccaga agattcctac ggcgagctga agcggatggc
     1141 gggcgacttc cagagttcgc tcgagcacta ccgcgcggcg ctgaagatcg atcccgactt
     1201 cgtgacttcg cagctgggac tgggcgacac ctatgccctg atgggaaatc aggagcaggc
     1261 gcgagctgag tatgacaagg cgatccgcta cgcccacaac gaagccgacc ggctgaccta
     1321 cggcatgcag aaggccatga cttacgtgcg cgacgggaac tttgccgaag cggacaagga
     1381 atatcaggag attgccgaga ctgcgcacgc caaggagcag gacctgcagg aagcgcaggc
     1441 gttcgggcgc atggccgagt atcagagcga cgacaatgcg gcgctgaaag atctgaagct
     1501 ggcggaagag gccctgggac atcgggcaac gatttcgcct gcggatcgtg acgaagaaat
     1561 gtcgcggatc ctgcgcaacc gtgccgtgcg cgctgcaagg gcagggaacc aggtgctcgc
     1621 tgacaagtct cagcagcagc tggaggccat ggccagcggc agccgcaacc gtgtgattca
     1681 agcctcctac aacggcgcag cgggcacgct cctgatggac cagaagaaat acgaggatgc
     1741 aatcgcgcat ctcgaagaag atcaggacaa tcccttcacc atggagctgc tggtgcaggc
     1801 ctactacgag acgggcgcta ccgaaaagct gcacgaaatg gaagccaagc tccgcgggac
     1861 caatgtcccg accatggagc aggcgctggt ggtgccggca gcccgggcca agcggccgaa
     1921 catctagact cacgccgatc ggcaggcgca tcatcccccg gacgagggcg tccggggctc
     1981 cacggatagg gcgctttagg ctccacagac cagggcttgc ggggctccac aaacgggggc
     2041 actctggcgt cactacctcg taccgcttga cggcttggtg tagagtagtt gggcaccaag
     2101 agccgatgat ggcccactcc gagcgccgtt ccggaaccag gatctcgacc cgcgtggcca
     2161 cacgtgtgcg cacctcccag ggcgcggacc acaccgccca gacccgagac gtcagcgcca
     2221 acggggtatt tctctacacc aagtcccgca tggagaaggg ctccgaggtc gagctggtgc
     2281 tgatccttcc gcccgagctt acttccggcg agaaatactg ggtgtgctgc caggcgacca
     2341 ttgtccgggt cgaggaagag ggatcggaat ttggagtggc ggcgcagatc cggcgcatgg
     2401 acattctgcc ggaagtcgcg gtgtaggccc ggcgacgcag ccttgggggg gcgagggcag
     2461 gcggacatct gctcgattag aggactccca aaaattcacg gttttctgag atctcgctta
     2521 ccttttgggc ggcgggggca gggaactgtg aaaaaaagct tcccattgca acggttccgg
     2581 tgcaataatg acggccctgg aattgctgtg cgtaattcgg gaatccggtg agtccgatat
     2641 gttgattgat aaactagcgg ccggcgtggt gcaggtgcaa acgccgatcg ggccgcgcta
     2701 tgtgatgccg tcattcaagc agcgagtgta cctgctgtgg gtgttccgca attttcccat
     2761 tcttccgcac gccgtgctca gccgccggca acagcgcatg atcgaccgca tgtgcagcga
     2821 gcagcggttc gcctccatgg cgtatgccga cggcatgaat gaggcgccgg tgatcggtac
     2881 ggtcgagcac cgtcctgcca tcagcccc
//
LOCUS       DASPUL010000594         4101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1337902, whole genome shotgun sequence.
ACCESSION   DASPUL010000594 DASPUL010000000
VERSION     DASPUL010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337902"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="VEJ00_13800"
     CDS             <1..1518
                     /locus_tag="VEJ00_13800"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXX02284.1"
                     /translation="PITSFDPQLTSTLQVDKNNTESVSVFSPVPVVAQNTYLANFAYT
                     QGFDWGTMLTAGFNNTHVTTNNPTSLLTPQLGSNFQLRLTQNLLQGFGLLPNTRFIRI
                     AKNNREISDVAFRLQVITTVDQVENMYWDLVFAFENVRVQRESLTYAQKALDDAQRQA
                     QVGTIPPIQVVSAQSTVATDQQNLIVAQNNLQLQQLLMKNAVSRSIEDPTLAEADVIP
                     TSTMQIPQEEPVIPIQDMISEALNHRAELVESRIDLNSRDINNKAVRNSMLPTVDAFA
                     YYGGSGVGGSVNPFVPVCSATSSKFCFDQATAPPPFQTTTRVSYGGTLNQLANSTAPD
                     KGIGLTLNIPIRNRLAQSNQVRAELEYRQALVREHQLENQVRIEVRNAGFDVKQNRVA
                     VQAAQSAVDLARQTYDADQQKLKVGLTTQTAILQDAATLTTSESNLVSAKAAYEKSRI
                     ELDRATGLLLDHAGIDIADATRGQVKRLPSVPYVAPRPDAGPTATPAQSGQPGQM"
     gene            1709..2494
                     /locus_tag="VEJ00_13805"
     CDS             1709..2494
                     /locus_tag="VEJ00_13805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXX02285.1"
                     /translation="MTLSVAITAMNEEANIGRTLASVSWADEIVLVDSGSKDRTCEIA
                     REYGARVVAEPWRGYVAQKQYAIDLCTKDWVLLLDADEEVSSGLADEIRAAIGDPNAA
                     VGYRLPRKNLFLGRWMRHGGFYPDPKLRLFRRGQGFVTGHDPHDRCELKPDMPQHTIQ
                     FRNGLVHYTYPNLTLYITHMNRYSSLGAQLAFAKGHTRFSVANILLRPLFTFIYNYFF
                     RLGFLDGREGLLLHCYHAGYVSWKYAKAWEMSRRDSQRGDHEC"
     gene            complement(2491..2817)
                     /locus_tag="VEJ00_13810"
     CDS             complement(2491..2817)
                     /locus_tag="VEJ00_13810"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HXX02286.1"
                     /translation="MAGRIVRGDVRLYQFAPPDKQRPVLVLTRESAISYLSTVTVAPI
                     TSTIRGVPSEVVLSEEDGMKALCAVNLHNAVTVSQQRLGKRVAQLRSQRMNEVCAALR
                     FSLGCD"
     gene            complement(2802..3035)
                     /locus_tag="VEJ00_13815"
     CDS             complement(2802..3035)
                     /locus_tag="VEJ00_13815"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HXX02287.1"
                     /translation="METIQVVLDKKLLRAADQAARRTRRNRSALVRDALRAHLRSLDV
                     QAREARDREGYVRQAPSHRESLLWEEEAAWPAE"
     gene            3664..>4101
                     /locus_tag="VEJ00_13820"
     CDS             3664..>4101
                     /locus_tag="VEJ00_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXX02288.1"
                     /translation="MEKKPSKLSNQPQVAESPLAEVFENRHPSDAEKQWAEKTLAPTL
                     EKAPEHPIGAPTGVNLDEHGHARFTTISGVPIRRLYTQADLPQDWSEESYLGFPGQPP
                     FTRGIHATGYRGKLFTMRQFSGFASPEETNQRYKYLLEHGGSGL"
BASE COUNT          857 a         1290 c         1174 g          780 t
ORIGIN      
        1 ccgatcacca gttttgatcc gcagctgacc agcacgctgc aggtggataa gaacaacacg
       61 gaatcggtaa gcgtcttcag cccggtcccg gtcgtcgcgc agaacacgta cctggctaac
      121 ttcgcctaca cccagggatt cgactggggt acaatgctga ccgcgggatt caacaacacg
      181 cacgtcacca ccaataaccc caccagcctg ctcacaccgc agctcggctc gaatttccaa
      241 ctccggctta ctcagaatct gttgcagggt ttcggcttgc tcccgaacac gcgttttatc
      301 cggatcgcga agaataatcg cgagatctcc gacgtcgcct ttcgcctgca agtgatcacc
      361 accgtggatc aggtcgaaaa catgtactgg gaccttgtct tcgctttcga gaatgtgcgt
      421 gtgcaacggg aatcgcttac ctacgcccag aaggcgctgg acgacgcgca acggcaggcg
      481 caggtgggca ccattccgcc cattcaggtc gtgagcgcgc aaagtacagt cgccaccgat
      541 cagcagaacc tgatcgtggc gcagaacaac ctgcaactgc agcaactgtt gatgaagaac
      601 gccgtcagcc gcagcatcga ggatccgact ctcgctgagg ctgacgttat tcccacctcg
      661 accatgcaga ttccgcagga agagccggtc attcctatcc aggacatgat cagcgaagcg
      721 cttaatcatc gcgccgagct ggtggaatcg cgcatcgacc tcaattcgcg cgacatcaac
      781 aacaaggcgg tacgaaactc catgctgccg acggtggacg cgtttgccta ctatggcggg
      841 tcgggcgtgg gaggctcggt caacccgttt gttccggtct gctcagcgac ctcgagcaag
      901 ttctgctttg accaggccac ggctccgccg ccttttcaga ccacgacgag ggtcagctac
      961 ggtggaacgc tcaaccagct ggccaattcg accgctccgg acaaaggcat tggcttgacg
     1021 ctgaatatcc cgatccgcaa ccggctggcg cagtcgaacc aggtgcgtgc cgaactggaa
     1081 taccggcagg cgctggtgcg tgagcatcag ctggagaacc aggtgcgcat cgaggtgcgt
     1141 aacgccgggt ttgacgtcaa gcaaaaccgg gttgcggtgc aggccgcgca atcggcggtc
     1201 gacctggcgc gccagaccta tgatgcggat cagcagaaac tgaaggttgg cttgaccacc
     1261 cagaccgcaa tcctgcagga tgcggcgacg ctcaccactt cggagtcgaa cctggtttcg
     1321 gccaaggcgg catatgagaa gtcgcgcatc gagctcgacc gcgcgaccgg tttgctactc
     1381 gatcacgccg gcatcgacat tgccgacgcc acccgcggcc aagtcaaacg cctgcccagc
     1441 gtcccgtacg tcgcgccgcg gccggacgca ggtccaacgg caacgccagc gcagagcggg
     1501 cagccagggc agatgtaggg gcttggggct agggactagt ggccagtggt cagtggtagt
     1561 ggcgagcgag gcctgggggt gcggttcact ggcccctagt ccctgttccc tgacccctgg
     1621 cccctggctg atagactcac agtgaaacca gagcatccgc cgtgcgggtt ttcaatcaac
     1681 ggtcaaaaat caacactcca caatctctat gactctttcc gtagccatca cggcgatgaa
     1741 cgaagaagcc aacattgggc gcacgctggc cagtgtgagt tgggccgacg aaatcgtcct
     1801 ggtggattcc ggctcgaaag accgcacctg tgagatcgcg cgcgaatatg gcgcgcgcgt
     1861 ggttgcggag ccgtggcgcg gttacgtggc gcaaaagcag tacgcgatcg acctgtgcac
     1921 gaaagattgg gtgctgctgc tggatgccga tgaggaagta agttccgggc tagccgatga
     1981 aattcgtgcg gccatcggcg acccgaacgc ggccgtcggc tatcgcctgc cgcgcaagaa
     2041 tctgtttctt ggccgctgga tgcggcacgg cggattttat cccgatccca agctgcggct
     2101 gttccgccgt gggcagggat tcgtcaccgg ccacgatccg cacgaccgct gcgagttaaa
     2161 gccagacatg cctcagcaca cgattcagtt ccggaatggc cttgtgcact atacctatcc
     2221 caatctcact ctttacatca cacacatgaa ccgctattca tcgctgggcg cgcagctcgc
     2281 cttcgcgaag ggacacacac gattcagtgt tgcgaacatc cttttgcggc cgctgttcac
     2341 tttcatctac aactactttt tccgcctcgg attcctcgac ggccgcgaag gcctgttgct
     2401 gcattgctat cacgcggggt atgtgtcatg gaaatacgcc aaggcgtggg agatgtcgcg
     2461 ccgagattct caacgtggcg atcacgaatg ctagtcgcag ccaagcgaga agcgcaaagc
     2521 ggcgcaaacc tcgttcatcc tctgcgaccg aagctgggct acgcgttttc ccagtcgttg
     2581 ttgcgacacc gtgaccgcgt tgtgcaggtt cacagcgcaa agcgccttca tgccgtcctc
     2641 ttcactgaga accacctccg agggcacgcc acggatcgtc gacgtaatcg gcgcaactgt
     2701 gaccgtggag agataagaaa ttgcgctctc gcgcgtgagc acgagcaccg gccgttgctt
     2761 gtcgggcgga gcgaactgat agaggcggac atctcctcgg actattctgc cggccacgcc
     2821 gcctcctctt cccaaaggag cgattcccgg tggctgggcg cctgtcgcac atagccctcg
     2881 cggtcccgtg cctcgcgggc ttgcacgtcc aggctccgga gatgcgccct aagggcgtcg
     2941 cgaaccaagg cagaacgatt ccggcgagta cggcgtgcag cctggtcggc ggcgcgcaag
     3001 agcttcttgt cgagaacgac ttggatggtc tccatatgtg gactccattc cacagtatac
     3061 cccacagccg ggacaagccg cgccgctgac gaccttgaac cttcttttca aagggaacga
     3121 aaaagctcgt gcgcaccttc cgtatttcta tttccgcttc gaatccttca tgccgcggag
     3181 cgcgcggcga gtcgcccacg ctattgcaag gaagattggc ccccaaatgg aaaccactac
     3241 gagcacggcg cgcaccgccg cacgctcttg aaacgctgcg accaggaggc gaccgatgaa
     3301 accgaaaaca aatatgccga gaattacgac gccagctctt gtgacaggcg tccagggttg
     3361 gcccccgaga ttacgccaaa accgcgcttc gttctgagcc gtgtcaggga atatgacatt
     3421 gcgctgacgc gcttcaatgt cccgggccca ttcctccgcg gaatatttgc agtcggtcat
     3481 gtactgagcg aatcttatcg ccgaagccgc tgacgcgctg tcgccatctt cggcgttgta
     3541 tcctcgtgtc ctctgcggtg aagctcttga cttcctaccg accggtcgtt ctcacccttc
     3601 ggctcgtaac cttgcccgat tctgcgcgct tccctatcat cagaactgag catcggagta
     3661 tgcatggaaa agaagccgtc caagttgtcg aatcagccgc aagtggccga gagcccgctg
     3721 gccgaggttt tcgaaaaccg ccatccttcc gacgccgaga agcaatgggc agaaaaaacg
     3781 ctcgcgccca cgcttgaaaa agctcccgag catccgattg gcgcgccgac gggcgtgaac
     3841 ctcgacgagc acggccacgc gcgcttcact acgatttccg gcgtccccat tcgacggctc
     3901 tacacccagg ccgatctgcc gcaagactgg tccgaagaaa gttatctcgg cttccccggc
     3961 cagccgccgt tcacgcgcgg cattcacgcc acgggatatc gcggcaagct tttcaccatg
     4021 cgccagttct caggcttcgc ttcgcccgag gaaacgaacc agcgctacaa atatctgctc
     4081 gagcacgggg gcagcgggct t
//
LOCUS       DASPUL010000595         3842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_522334, whole genome shotgun sequence.
ACCESSION   DASPUL010000595 DASPUL010000000
VERSION     DASPUL010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522334"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1265)
                     /gene="bshA"
                     /locus_tag="VEJ00_13825"
     CDS             complement(87..1265)
                     /gene="bshA"
                     /locus_tag="VEJ00_13825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HXX02289.1"
                     /translation="MTKHQEGGTPYMKIGITCYPTYGGSGVVATELGLELAQRGHEIH
                     FISYAQPIRLRGAEPNIHYHEVEVSRYPLFDYPPYDLALATRMAEVAQLYDLDLLHVH
                     YAIPHSVSALLARQMLAVGPRGRRLPFVTTLHGTDITLVGLDRSYLPITRYSIEQSDG
                     VTAISNYLRDRTQRVFDVKNRIEVIYNSVNCDVYKRDPETVATLRAEYAPNGQRVLVH
                     LSNFRPVKRLTDVIEIFDRVRKQIPSRLLLIGDGPDRSVAEWLAVQKGIHDDVLFLGK
                     QDQIYDKLAVADIMLMPSELESFGLAALEAMACEVVPIATRAGGVPEVIDHGKSGYLA
                     DVGDVETMARYAIELLSNEQRLREMAKQARAVAMERFCSTKIVKQYEDFYRRVLERSA
                     "
     gene            complement(1338..2018)
                     /locus_tag="VEJ00_13830"
     CDS             complement(1338..2018)
                     /locus_tag="VEJ00_13830"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXX02290.1"
                     /translation="MSAQGSAPRATLDLKVRVWGMGSNNQPFFQNAVAQNISATGACI
                     YGIEPELKVGDVIGVQYEGKKARCKVIWVVDAGALKKTQVGVQLVADQECPWTGALPH
                     DMKTEERTQATQDNRRKFARHKISFPLELRDERVNTPLRVNATDISGNGCYVETVMPL
                     AVGTALRVDFWMEEERLSPSAIVRTRDPGVGMGIEFTGLPDENKKRFQAHLDKLDPAT
                     VLGHKSAQ"
     gene            complement(2178..3059)
                     /locus_tag="VEJ00_13835"
     CDS             complement(2178..3059)
                     /locus_tag="VEJ00_13835"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HXX02291.1"
                     /translation="MRRLIINADDFGFTEGVNRAIVEAHTRGVVTSSTLMANGTAFAD
                     AAELVKSVPQMSIGCHIVLIDGKPTLSPDRIPSLTKPTRAQEFRDGLKGFAACALAGR
                     LTAKEITAEAAGQMRKIQDAGIVLTHFDTHKHTHIFPAILRPLLQAAADCSVRAVRNP
                     FGPRLPLRSSQLLKRPNLWTRYAEVKVLRRFASKFRAAVDREGFATPDGTLGIEVTGA
                     LDETLFTAIARNIPDGTWEFVCHPGYNDAELQSARTRLRESRETELHVLTLPRAREVL
                     AQAGVELISYRALAGKP"
     gene            3262..3338
                     /locus_tag="VEJ00_13840"
     tRNA            3262..3338
                     /locus_tag="VEJ00_13840"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3296..3298,aa:Val,seq:tac)
     gene            3475..>3842
                     /locus_tag="VEJ00_13845"
     CDS             3475..>3842
                     /locus_tag="VEJ00_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02292.1"
                     /translation="MTLDPQTTFSLFNAIATAGRTIYDIAQGTTKLEEKQQLMAVYDQ
                     LMNLKRSAADLEDENYGLKQKLRFKGDEFEFKNPFWFEKKHPDRALCPKCWSKQIAAP
                     VGQPYDNDFGIFRRCLTCDTP"
BASE COUNT          761 a         1147 c         1141 g          793 t
ORIGIN      
        1 accatcgata gccgcgaagg caagggaact ctcattcgca tccggctgcc cctggtcgaa
       61 gccgccagct cggttccgga gggtttctac gccgagcgtt ccagcacgcg gcggtaaaaa
      121 tcttcgtact gcttgacaat cttggtggag cagaaccgct ccatggccac ggcccgggcc
      181 tgtttcgcca tctcgcgcag gcgctgttcg ttgctgagga gttcgatggc gtagcgcgcc
      241 atggtttcca catctccgac atcggcgaga tatccgctct tgccgtggtc gatgacttcg
      301 ggcacgcccc cggcgcgcgt cgcaatgggt accacctcgc aagccatcgc ttccagtgcc
      361 gccaatccga acgattccag ctcgctcggc atcaacatga tgtccgcgac ggccagcttg
      421 tcgtaaatct gatcctgctt gcccaggaag aggacgtcat cgtgaatgcc tttttgcaca
      481 gccagccatt ccgccaccga gcggtcggga ccgtccccga tcagcagcaa tctggaggga
      541 atctgcttgc gcacgcgatc gaaaatctcg atcacgtcgg tgaggcgctt caccggacga
      601 aaattggaca ggtggacaag aacgcgttgg ccgttgggcg cgtactcagc ccgtagtgtc
      661 gctacggttt ccggatcgcg cttatagacg tcgcagttca ccgagttata gatcacttca
      721 atgcggttct tcacgtcgaa gacgcgctgc gtgcgatcgc gaaggtagtt ggaaatcgcg
      781 gtgacgccgt cactctgctc gatcgagtag cgagtgatgg gcagatacga ccggtcgagg
      841 ccgaccagcg tgatgtcagt cccgtgcagc gtggtaacga aaggcagtcg ccgcccgcgc
      901 ggcccaaccg ccagcatctg ccgcgccagc aacgcactca ccgaatgcgg gatagcatag
      961 tgcacgtgca gcagatcgag atcgtaaagt tgcgcgacct ccgccatgcg tgtcgccagc
     1021 gccagatcgt aaggagggta gtcgaacaac ggataacgcg atacctcgac ttcatgatag
     1081 tgaatgttcg gttccgcccc gcgcaggcga atgggctgag cgtacgaaat gaaatgaatc
     1141 tcatggccgc gctgtgccag ctccagaccg agttccgtgg ccacgacacc gcttccgccg
     1201 tacgtcggat aacacgtgat cccgatcttc atgtaggggg ttccgccctc ttggtgcttt
     1261 gtcattcgat ggctgcaagg tgtggcggtt gcagatttta cacgaacggt cggcaggtct
     1321 ctcgccggaa agacttgtta ctgggcgctt ttgtggccca gtacggtggc cggatcgagc
     1381 ttgtcgagat gcgcctggaa gcgctttttg ttctcatccg gcaggccggt gaattcgatt
     1441 cccatcccga ctccggggtc gcgggtgcgc acgatcgcgc tgggcgagag ccgctcttcc
     1501 tccatccaga agtcaacgcg caacgcggtt cccacggcca gcggcatgac ggtctccaca
     1561 taacagccat tcccgctgat gtctgtggca ttcaccctca gcggcgtatt cacacgctca
     1621 tcgcgtaact ccagggggaa agagatcttg tggcgggcaa actttctgcg gttgtcttgc
     1681 gtggcctgcg ttcgctcttc cgtcttcata tcgtggggaa gagctccggt ccaggggcac
     1741 tcctggtcgg ccaccaactg aacgccgacc tgggttttct tcagcgctcc cgcatccact
     1801 acccagatga ctttgcagcg ggccttcttg ccttcgtatt gcacgccgat gacgtcgccg
     1861 actttcagct ccggctcgat gccataaatg caggcgccgg tcgcgctgat gttctgagcg
     1921 accgcgttct ggaagaacgg ttgattgttg gagcccatgc cccacactcg gaccttcaag
     1981 tccagagtcg cacgcggcgc tgacccttgg gccgacatga gggcgaacct cccgcacaga
     2041 gaaacggtga attgtacccc atgatagcgg cttcaacata acgatggagg ttacgacagt
     2101 actttcgaac ccgccggaga ggctagcccg gcaaacgccg ggcgccggcc cggcatgcgc
     2161 ggaaaggcgc tccggcgcta tggtttgccg gccagcgccc ggtacgagat gagctcgacg
     2221 cctgcctgcg cgaggacctc ccgcgcgcgc ggcagagtca ggacgtgcag ttctgtctcg
     2281 cgcgactccc gcaagcgagt cctggcggat tgcaattcgg catcgttgta accggggtgg
     2341 caaacgaact cccaggtgcc atccgggatg ttgcgcgcga tggcggtgaa caaggtttcg
     2401 tcgagcgccc cggtgacttc aatgcctaat gtgccgtcgg gtgtggcaaa gccctcgcgg
     2461 tccactgccg cgcgaaactt tgaagcaaat cgcctcagga ccttgacctc ggcatagcgt
     2521 gtccacaggt tcggccgctt cagaagctgg ctggaccgca agggaaggcg tggcccgaac
     2581 gggttgcgca cggcacgaac actgcagtcg gctgccgcct gaagcaacgg ccgcaggatt
     2641 gcgggaaaga tgtgcgtgtg cttgtgcgtg tcaaaatgcg tcaggacgat gccggcgtcc
     2701 tggatcttgc gcatctgccc agcagcctcg gcagtaattt ccttcgcggt gagtcggccg
     2761 gcgagcgcgc acgcggcaaa acccttgagc ccgtcgcgga attcttgcgc ccgggttggc
     2821 ttggtgaggg aagggatccg atcggggctc agcgtgggtt taccgtcgat cagcacaatg
     2881 tggcagccaa tgctcatttg tgggaccgac ttgaccagct ccgcggcatc ggcaaaggca
     2941 gtaccattgg ccatcaatgt tgacgatgtc acgaccccgc gcgtgtgggc ctcaacgatg
     3001 gcccgattca cgccttctgt aaaaccaaag tcatccgcgt tgatgatgag acgccgcacc
     3061 cacgcagtct agcagggtga cgacgttcac aaagcgcgga ctgccataag cgcaaagccg
     3121 gcatcacccc gtacctgacg agtgcgagct ttgtttgccc ggcttttccg agcttgcttt
     3181 atactcggaa aagcgtctct tgtcgcgcgc cctcacgaat ttccgggtgc agcgtcagtc
     3241 ctgattcgcg cccacccgag ggggcggtta gctcagctgg ttagagcgcc tgccttacaa
     3301 gcaggaggtc gcaggttcga gtcctgcact gcccaccatg tttttcaaca acttgtgaag
     3361 catcgccgat gctgctaatg gagcgtttta ccccttcgtt ttacctttcg acgaacagaa
     3421 caagaaagtc ggttttccac ggatccgcga ggttcgcggt aatctgaggg cattatgacc
     3481 ctagatcctc agaccacatt tagcctattc aatgcgatcg cgacggctgg tcgcaccatc
     3541 tacgacattg ctcaaggaac gacaaagctc gaggagaaac aacagctgat ggctgtctac
     3601 gatcagctga tgaacctgaa gcgcagtgct gccgatctag aagacgagaa ctacggcctg
     3661 aagcaaaagc tccgtttcaa gggcgacgag tttgagttta agaatccatt ttggtttgag
     3721 aagaaacacc ctgacagagc gttgtgtccg aagtgttggt ccaagcagat cgcagctcct
     3781 gtggggcaac cttatgacaa cgactttggg attttccgtc gttgcttaac ctgcgatacg
     3841 cc
//
LOCUS       DASPUL010000596         2992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_664212, whole genome shotgun sequence.
ACCESSION   DASPUL010000596 DASPUL010000000
VERSION     DASPUL010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664212"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            477..2471
                     /locus_tag="VEJ00_13850"
     CDS             477..2471
                     /locus_tag="VEJ00_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02293.1"
                     /translation="MTNWTGIPRPWLTIVLVLSTTSSLLAATVTVSPERAAITLGTQT
                     QQFISSVPNVIWKVDGVVGGNAKVGTISATGLYTPPVTAGVHTITATTVAAPVSSGSA
                     TIGVTDLAGVFTYHNDTARDGVNLHEFALSPRTVNTTMFGKLFSCAVDGAVYAQPLWV
                     HGLTIAGKVHNVIFVATQHDSLYAFDADANPCVMYWHVNLLDTLHGGTSGDRPVTWDD
                     VGSCFGDIYPEAGVTGTPVIDPATSTIYLVSASERNPSNTGNCASTVGSFAHRLHALD
                     LATGTEKFSGPVTIKASVLGSGDASVNGIVSFNSQLHNQRSGLALAGGNVYVAFASHE
                     DAMPYHGWLIAYKASDVQVQTAVFNTTPNGLNGADGGIWAGGGAPAVDSVGDIYVSTG
                     NGVFDEAPPPRDNDYGDSILRLHPFSGHTLNGVNLSVAGWFTPDDQLSLAESDTDLGS
                     GAVVLLPNQTSGAGPKHLLTLVGKEGVVYLIDRDNMGQFNSASNNQIVQSFSGPAGLW
                     GTPAFWKNNLYVGGAGDSLRQYTFDPSMELYNTPAASQSSQLFNFPGATPSISSPGLW
                     ARGIVWAIDASQYGYASGNAPNGINCATVPVPAACTGPAVLHAYAAENLAVEYWNSSM
                     AANHRDRAGNGVKFVPPTVANGRVYVGTRTEVDVYGLLPN"
     gene            complement(2481..>2992)
                     /locus_tag="VEJ00_13855"
     CDS             complement(2481..>2992)
                     /locus_tag="VEJ00_13855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HXX02294.1"
                     /translation="AVARVLQQLAMMIGARMVFELGSAIGYSTIWWAQAVGERGRVIY
                     TDGDSKNAERARGYFSRAGVANRVQLHVGDALEFLSEQKQEFDVIFNDVDKEDYPRVL
                     RLVAPRLRKGGLFVTDNVLWSGRVAEKNPTDARTRAILEFNRKLYDSPEFYTTILPIR
                     DGLAVAVKK"
BASE COUNT          608 a          906 c          893 g          585 t
ORIGIN      
        1 aggcagagcc gatgtgctgg gtgcgaactg aaggtcgcga agcaggtgcg atccggcata
       61 aatcaccccg ataatgaaga cgattccgaa aacgaccaca ccgatcatgc cgggggaacg
      121 tgaaatcttc tggtcgagcg cagttgtcgc agtagccata gacgatagtc tcccgaagaa
      181 atttgacttt cttgatccgt gcttcgaatc tggattcggg gactatattt gcggaatgtt
      241 acgggaaggt tagcggaatg ttacacgacg attaagattg gccggaatac cctgatgccg
      301 acggatccgg aaaggcggaa atcgacgcgt cacgaccctc cagcggtaac gaaatgtgaa
      361 cttcctattt ttctgcagct gcagacccga ggggccgcag gtacaatggg gccggctgtc
      421 attcctgcac tgaaacccct ttgccgaaaa actgcctgat tgggaggcgg atacaaatga
      481 cgaattggac ggggattccc cggccctggc tcaccatagt cctggttctg agcaccactt
      541 catcgctgct ggccgcaacc gtcaccgtgt cgcctgagcg agcggccata acgctaggta
      601 cgcaaacgca gcagttcatt tcctcggtcc cgaacgtgat ctggaaggtc gacggtgttg
      661 tgggaggcaa cgccaaggtt ggtaccatct ccgccacagg cctgtacact cccccggtga
      721 ctgcgggtgt tcacacgatt acagcgacga ccgttgctgc cccagtatcc tcaggctcag
      781 ctacgatcgg cgttactgat ctggcgggtg tgttcaccta ccacaacgac acggcgcgcg
      841 acggagtgaa cctgcatgag ttcgcgctca gccctcgaac ggtgaacaca accatgtttg
      901 gaaagttgtt ttcctgcgcc gtggatgggg cggtttacgc ccagccgctg tgggtgcacg
      961 ggctgaccat cgccggtaaa gtgcacaacg tcatcttcgt ggcgacccag catgacagcc
     1021 tctacgcctt cgatgccgac gcgaacccct gcgtaatgta ctggcatgtg aacctgctcg
     1081 acacactgca cgggggtacc tcgggagacc ggcctgtgac ctgggatgac gtcggcagct
     1141 gcttcggcga catctatccc gaggcaggag tcaccggcac accggtgatc gatccggcaa
     1201 ccagcaccat ctacctggtg agcgcgtcgg aacgcaatcc ctcgaacacc ggaaactgcg
     1261 cctctacggt ggggagcttt gctcaccggc tgcatgccct ggatctggcc acgggaacag
     1321 aaaagttcag tgggccggtc acaatcaagg cttctgtgct tggaagcggc gacgcatcgg
     1381 tgaacggcat cgtgagtttc aactcgcagc tgcacaacca acggtctggc ctggcgctgg
     1441 ccggcggcaa tgtctacgta gcgttcgcct cccacgagga cgcgatgcct tatcacggct
     1501 ggttgattgc ctataaagcg tctgacgtgc aggtgcaaac cgctgtcttc aacaccactc
     1561 cgaacgggct gaacggagcc gatggcggca tctgggcagg cggcggcgcc cccgcggtcg
     1621 acagcgtcgg cgacatttat gtcagcaccg gcaacggcgt ttttgacgaa gccccgcctc
     1681 cacgcgacaa cgactacgga gacagcattc tgcggctgca tccgtttagc ggccacaccc
     1741 tcaacggagt caacctgagt gtggcgggct ggtttacccc cgatgatcaa ttgtccctgg
     1801 cagagagcga caccgatctg ggctcgggag cggtggtctt gctgccgaat cagacgtcgg
     1861 gcgccggccc caaacatttg ttgaccctgg taggcaaaga gggcgtcgtc tatttgatcg
     1921 accgcgacaa catgggccaa ttcaattcag cgagcaacaa tcagattgtg cagagctttt
     1981 ccgggccggc tggcttgtgg ggtactcctg cattctggaa gaacaacctg tacgtcgggg
     2041 gtgcaggcga ttcccttcgg cagtacacct tcgatccatc catggagttg tacaacacgc
     2101 ccgccgcgtc gcaatcgagc cagctgttca acttccctgg cgcgacacct tcgatctctt
     2161 ctccgggatt gtgggcgcgc ggcattgtgt gggcgatcga tgccagccag tacggctacg
     2221 ccagcggcaa tgcacccaat gggatcaatt gcgcgaccgt gcccgtgccc gcggcctgca
     2281 ccggcccggc cgtcttgcac gcctatgccg ccgagaattt agcggtggaa tactggaata
     2341 gttcaatggc ggccaatcat cgcgaccggg ccggcaacgg agtgaagttc gttccgccca
     2401 cggtggcgaa tggcagggtc tacgtcggaa cccgcaccga ggtcgatgtc tacgggctgc
     2461 tgccgaacta agcggactct ctacttcttc acagccaccg ccagcccgtc gcggatgggc
     2521 aggattgtgg tgtagaactc cggcgagtcg tagagcttgc ggttgaattc gaggatcgcc
     2581 ctggtcctgg catcggtcgg attcttctcc gcaacccgcc cgctccacag cacgttgtcg
     2641 gtcacgaata atccgccttt gcgcaaccgg ggtgcgacca ggcgcaacac gcgcggatag
     2701 tcttccttat ctacgtcgtt gaagatgacg tcgaactcct gcttctgctc ggagagaaat
     2761 tcgagcgcat cgccgacgtg gagctgtaca cggttggcga ccccggcgcg cgagaaatat
     2821 ccgcgggcgc gctcggcgtt cttcgaatcg ccgtcggtgt agatgacgcg gcctctctca
     2881 cccaccgcct gcgcccacca gatcgtggaa tagccaatgg cggagcccaa ctcgaagacc
     2941 atacgggcgc cgatcatcat ggccaactgc tgcaggacgc gcgccaccgc cg
//
LOCUS       DASPUL010000597         2422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_523272, whole genome shotgun sequence.
ACCESSION   DASPUL010000597 DASPUL010000000
VERSION     DASPUL010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523272"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1384)
                     /locus_tag="VEJ00_13860"
     CDS             complement(83..1384)
                     /locus_tag="VEJ00_13860"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX02295.1"
                     /translation="MEPEPEQGTATISLASYARLIGGNRNFRRVWLAQIVSEIGDWFY
                     TLSIYTLLLQLTGHAGSVALALVLQVLPQTLVGPTAGVVNDRLRRKHVMIAADLIRFV
                     VVLAMLFVRSRSMVWLVYPLLVAETSMAAFFEPARSAVIPNLASAEEVLVANTLSSAT
                     WSVNLVIGASVGGVVAAFFGRDAVFILNALSFLASAALIAGMRFPEPHAESAPPLRAR
                     DLVDFSPVLEGIRYIRSHRTLLPAVFAKAGELMVGPSWVIFTVLGAREFAVHWRGIDS
                     ARGAMLGMSILLGGRGLGALVGPIAGARWAHRSDHRLRLGILFGYLTIATGYGLLGVS
                     RSVWAAASCAMLAHAGGSTVWVFSTTLLQLHAEDRFRGRVFAADLGMSSLSFAITAYL
                     TGILLDSGVPVRTVASGTGFVMLIPAAIFALALRANAASRE"
     gene            complement(1471..>2422)
                     /locus_tag="VEJ00_13865"
     CDS             complement(1471..>2422)
                     /locus_tag="VEJ00_13865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HXX02296.1"
                     /translation="ACAIEAQRIGLRVIVVDKGCVVNSLFHYPANMVFFTTPELLEIG
                     DIPFTTAQQKPTREEALEYYRKVAEHYRVDVRQYEWVKTVLGEDGDFTITATDRAGRP
                     HDYRAKKVIVSTGYYDLANKLGIPGEELPKVSHYYREPHPYYDADVLVIGGKNSAAIA
                     ALDLWRHGGRVTLVHREPQIHHHVKYWVKPDLENRIKNGEIEAYFSSWVQEIGVDHVV
                     INTPGGPLRLKNDFVLAMIGYHPDYDFLRSMGIELSEQQCRPVCDPVSLESNVKGIYV
                     AGVIVAGSRTNEIFIENGRFHGKQIAEDLRIKLAASGRTE"
BASE COUNT          520 a          769 c          709 g          424 t
ORIGIN      
        1 ataggtttga aagggcgcgg cttctagacg cgccgctcgg cccaagaagg atgcgggctt
       61 tagcccctga ggaaagaaac tcttactctc tcgaagctgc attcgcccgc aacgcaagcg
      121 cgaaaatggc ggcgggaatg agcatgacga acccggtccc gctagccacc gtgcgaaccg
      181 gcactccgga atcgagcaag atgccggtca gatacgccgt aatcgcgaac gacaggctcg
      241 acatgcccag gtctgccgcg aatactcgcc cacgaaagcg gtcttctgcg tgaagttgca
      301 acagcgtggt tgagaagacc cacaccgtcg aacctccggc atgcgccagc atcgcgcacg
      361 aagccgccgc ccacacggat cggctcacgc ccagcagccc gtatcccgtg gcgatggtga
      421 ggtagccaaa aagaattccc aggcgcaggc gatggtcgct gcgatgcgcc cagcgcgctc
      481 cggcaatcgg accgaccagc gctcccagac cacgaccacc cagaaggatg ctcattccga
      541 gcattgctcc ccgcgccgag tcgatgccgc gccagtgcac cgcaaactca cgagctccga
      601 gcaccgtaaa aataacccag ctcggcccca ccatgagctc tccagccttc gcaaacacag
      661 ccggtagcag cgtgcgatga cttcggatgt agcgaatgcc ttccagtacc ggcgagaagt
      721 cgaccagatc gcgggcgcgc agtggcggcg cagactcagc gtgcggttcg gggaagcgca
      781 ttcctgcgat cagcgccgcg gatgcaagaa acgacaacgc attgaggatg aagaccgcgt
      841 cgcgcccaaa gaaagcagct acgactccgc caaccgaagc cccaatcacc agattcaccg
      901 accaggtcgc cgacgacagc gtgttggcga ccagaacttc ttccgctgaa gcgagattcg
      961 gaatcactgc actgcgcgcc ggttcaaaaa aagccgccat cgaggtctcg gccaccagca
     1021 gcggatacac cagccacacc atcgaccgcg aacgcacgaa cagcatggcc agcaccacta
     1081 cgaagcggat caggtcggcg gcaatcatga cgtgcttgcg gcgcagccgg tcgttcacca
     1141 ctccggccgt tggtccaacc agcgtctgcg gcagaacctg caacaccaac gccagcgcca
     1201 ccgaaccagc gtgccccgtg agctgcaaca gcagcgtgta gatcgagagc gtgtagaacc
     1261 agtccccgat ctcgctgacg atctgcgcca gccagacgcg gcggaaattg cggtttccgc
     1321 caatcagccg tgcgtaagac gccagcgaga tcgtagctgt gccctgctcc ggttcgggtt
     1381 ccatgcgtct ggctaggtta aacggtggcg cggacaccgg caaaggggga tggactgggg
     1441 cctaagaagc tgttgttgag gttcgaaccg ctattcggtg cggccagagg ctgctagttt
     1501 gatccgcaag tcctcagcga tctgtttgcc gtggaagcgc ccgttctcga tgaagatctc
     1561 gttggtgcgc gagcccgcga caattacacc ggcgacgtag attcctttca cgttgctctc
     1621 gagcgaaacc ggatcgcaga ccgggcggca ttgctgctcc gacaattcaa tgcccatgct
     1681 gcgcagaaaa tcatagtcgg ggtggtaacc gatcatggcc agcacgaaat cgttctttag
     1741 ccgcagcggc ccgccggggg tgttgatcac aacatgatcg acgccgatct cctgcaccca
     1801 gctgctgaaa taagcctcga tttcgccgtt cttgatgcgg ttctccaggt cgggcttgac
     1861 ccagtacttg acgtgatgat ggatttgcgg ctcgcggtgc accagcgtga cccgcccgcc
     1921 gtgacgccag agatcgagcg cggcgatcgc agcagaattc ttcccgccga tcaccagcac
     1981 atccgcgtcg taatagggat gcggctcgcg gtagtagtgc gagaccttgg gcaattcctc
     2041 tcccggaata cccagtttgt tcgcgagatc gtagtagccg gtggaaacaa tgaccttctt
     2101 cgcgcggtaa tcgtgcgggc gcccggcgcg gtcggtcgct gtgatcgtga aatctccgtc
     2161 ttcgcccagc acggtcttga cccactcata ctggcgcaca tcgacgcggt agtgctcggc
     2221 gactttgcgg taatactcga gagcttcttc gcgtgttggt ttctgctggg cggtcgtgaa
     2281 gggaatgtcc ccgatttcca gcagctctgg cgtcgtaaag aacaccatgt tggcgggata
     2341 gtgaaacaga gagttcacca cgcatccctt gtcgaccacg atgacccgaa gcccgattct
     2401 ctgcgcttcg atcgcgcacg cc
//
LOCUS       DASPUL010000598         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1614140, whole genome shotgun sequence.
ACCESSION   DASPUL010000598 DASPUL010000000
VERSION     DASPUL010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614140"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VEJ00_13870"
     CDS             complement(<1..1072)
                     /locus_tag="VEJ00_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HXX02297.1"
                     /translation="MPIRWDKFTVKAQEAVQRASQLASEHGNPELQPVHLLAALLEDK
                     EGIVPPVLEKIGIGPQAALSQIYGEIDKLPKVSGEAAQATLSNEVNKLLDQAFKEAAN
                     FKDEYVSTEHLLLAITHLKRDKAQQILAQHGATYDAILKALTAVRGSQKVTDQNPEAK
                     YQALERYARDLTEQARRGKLDPVIGRDEEIRRVVQVLSRRTKNNPVLIGEPGVGKTAI
                     VEGLAQRIISGDVPEVLKNKRVVALDLGAMLAGAKYRGEFEDRLKAVLREIEDSQGQI
                     ILFIDELHTLVGAGAAEGAIDASNMLKPALARGELRAIGATTLNEYRKYIEKDAALER
                     RFQIVFVGEPNVEDTIAILRGLK"
     gene            complement(1304..1801)
                     /locus_tag="VEJ00_13875"
     CDS             complement(1304..1801)
                     /locus_tag="VEJ00_13875"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXX02298.1"
                     /translation="MTVLTRWEPFREFATLQDRMNRLFRESFNEGRDESLTTTSFAPA
                     VDVYEDEHKVALKIEVPGIEEKDIDVRVENNTLTVHGERKIEKEEKEENYRRVERQYG
                     SFTRTFTLPTTVDTENVSANYDKGVLKITLPKKAEAKPKQIKVHVGSEKGEKTLEAKA
                     PGKAA"
     gene            complement(1973..>2299)
                     /locus_tag="VEJ00_13880"
     CDS             complement(1973..>2299)
                     /locus_tag="VEJ00_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit methyltransferase I"
                     /protein_id="HXX02299.1"
                     /translation="ASKREYDIIVLDPPAFAKTRRDLDSAMRGYKELNLRALKMLRSG
                     GTLVTCSCSYHVDQSSFLAMLADAALDARRTLRLIEVRGQAKDHPVLLNVPETAYLKC
                     LIATVT"
BASE COUNT          387 a          652 c          709 g          551 t
ORIGIN      
        1 ccttcaggcc gcgcagaatc gcgattgtgt cttcgacatt cggttcgccg acgaagacta
       61 tctggaagcg gcgttcgagc gcggcgtctt tctcgatgta cttgcggtat tcgttgagcg
      121 tggtggcgcc gatagcgcgc aattcgccgc gagccagcgc gggcttgagc atgttggagg
      181 cgtcaatcgc tccttcggcc gctcccgcgc cgaccagcgt gtgcaactcg tcgatgaaga
      241 ggatgatctg gccctgcgag tcttcgattt ccctcaagac cgccttcagc ctgtcttcaa
      301 actcgccgcg atactttgct ccggccagca ttgcgcccag gtcgagcgcg accacgcgct
      361 tgttcttgag aacctcgggg acatcacccg agatgatgcg ctgggccagg ccctctacga
      421 tggcggtctt cccgacgccc ggctcaccaa tcagcacggg attgtttttc gtgcgacgag
      481 agagcacttg gactacgcgg cggatttctt cgtcgcgtcc gatgacggga tcgagcttgc
      541 cgcgccgagc ctgctcggtc aggtcgcggg cgtagcgctc gagcgcctgg tattttgctt
      601 cgggattctg gtcggtgact ttctgcgatc cgcggacggc agtcagcgcc ttcaggatcg
      661 cgtcgtaggt agcgccgtgc tgcgccagaa tctgctgcgc cttgtcgcgc ttgaggtgcg
      721 tgatggcgag cagcaggtgc tcggtcgaaa catattcgtc cttgaagttt gccgcttcct
      781 tgaacgcctg gtcgagcagc ttgttgactt cgttcgagag cgtggcctgg gccgcttcgc
      841 cggacacttt gggcagcttg tcgatctctc cgtagatctg gctcaacgcc gcctgcggcc
      901 cgatgccgat tttctccagc accggcggga caatgccttc cttgtcttcc agcagcgcgg
      961 cgagcaagtg cacgggctgc agttcaggat tgccgtgctc cgaggcgagc tggcttgcgc
     1021 gctggaccgc ttcctgcgcc ttgacggtaa atttgtccca acggataggc atgggttatt
     1081 ttcctgcttc ctttgtcatt ctgagcgaag cgaagaacct atgcaatgtg tcggcaacgc
     1141 ggcaaaaatg cacaggtcct tcgctgcgct caggatgaca gaacaaacac gatttcaaaa
     1201 taaacgggag gcggtcacct ctgccgcctc ccggtaccac aggggctaaa gcccgcattt
     1261 gtttcctgcc ttatcgcggc gctaaagcgc cgcccttcca caattacgcg gccttgccgg
     1321 gggctttggc ttccagggtc ttttcgccct tttcgctgcc cacatggact ttgatctgct
     1381 tgggcttggc ttcggccttc ttgggcaggg tgatcttcag cacgcccttg tcgtagttcg
     1441 ccgagacgtt ctcggtgtca acggtggtgg gcagagtgaa ggtgcgggtg aagctgccgt
     1501 actggcgctc cacgcggcga tagttctctt ccttctcttc cttctcgatc ttgcgctcgc
     1561 cgtgcacggt aagggtgttg ttttcgacgc ggacgtcgat gtctttctcc tcgattccgg
     1621 gcacttcgat cttaagtgcg accttgtgct cgtcctcgta gacgtcgacg gcgggggcaa
     1681 agctggtggt ggtcagggat tcgtcccggc cctcgttgaa ggactcgcgg aacagacggt
     1741 tcatacggtc ctgcagggtg gcaaattcgc ggaacggctc ccaacgggtt aatacggtca
     1801 tggctacttc ctccttcgga attttggttg agcccaacgg ctcgattaat ttcgcgaccg
     1861 gcctctggaa tggcttcggg cggcagtcac tacaattaga cgcgtaatta gcgtaaagta
     1921 agcataaaac ttgagtgttc cattgtcaag catgatatgt gtttattttt ctttaggtta
     1981 cggttgcgat caggcacttg agataagccg tttcaggcac attcagcagg actgggtggt
     2041 ccttggcctg gccgcggact tcgatcaggc ggagggtgcg gcgggcgtcg agggcggcat
     2101 cggcgagcat ggcgaggaag ctggactggt ctacatggta agagcacgaa caggtgacaa
     2161 gggttccacc cgaacgcaac attttcagcg cacgcagatt cagctcttta tatccgcgca
     2221 tggcggaatc gaggtcgcgc ctggtcttgg cgaaggcggg cgggtcgagg acgatgatgt
     2281 cgtattcgcg cttggacgc
//
LOCUS       DASPUL010000599         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1481805, whole genome shotgun sequence.
ACCESSION   DASPUL010000599 DASPUL010000000
VERSION     DASPUL010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481805"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VEJ00_13885"
     CDS             complement(<1..377)
                     /locus_tag="VEJ00_13885"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXX02300.1"
                     /translation="MTHLSAETNFQTYNSPEVASHYAALDYLTPCERLLFETYVPVGG
                     AVLDLGVGGGRTTPYLASRASRYVGVDYAEAMVEACRAKFPAVEFIITDASDLSGFPD
                     SCFDAVVFAFNGIDLLLPDKTRRA"
     gene            complement(385..1476)
                     /locus_tag="VEJ00_13890"
     CDS             complement(385..1476)
                     /locus_tag="VEJ00_13890"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXX02301.1"
                     /translation="MKIVSPFLKRVLYPSLSAAGAFHRIGARGLAVVTYHGVLPKGYE
                     SVDPSLDGNLVAAEALRRQLQLLKAHYDVIAPEDLLAWREDRGQLPARAVLLTCDDGL
                     LNCLTDMLPVLQQERLRCLFFVTGASTTEIRNTLWYEDLMRLFLRAPAGSFEISGGGF
                     PSSGELREREQRLTVWWNYVKQLSRVESETRRSFLSVARTQLGSSSWQPFDGEDSLSC
                     RRFGLLTRTELCQLAAAGMTIGAHTLSHPVLSQAPPDLARTEISESRAQLEAVLQRQV
                     WAFAYPFGDPQSVTPQVLAMAQEAGYAAAFLNFGGGLGADVPPYALPRLHVTADMGLA
                     EFEAQVSGFHAWLQRRAGRDALPVGTVQV"
     gene            complement(1473..>2090)
                     /locus_tag="VEJ00_13895"
     CDS             complement(1473..>2090)
                     /locus_tag="VEJ00_13895"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HXX02302.1"
                     /translation="AQTGKPLIISTGMATVEEIEEAVGTARQAGAAQIALLKCASAYP
                     SPPEEMNLTTIPELARRFGVPVGLSDHTLGIAVPVAAVALGACIIEKHLTLSRSEPGP
                     DSAFSLEPEEFRAMVDAVRVAEKALGEVHFGFSPHEASSRVFRRSLFVVCDIKQGEVF
                     TAQKVRSIRPGYGLHTRHLTEILGQHAARDIERGTPFSWDMISRQ"
BASE COUNT          444 a          672 c          599 g          375 t
ORIGIN      
        1 gctcgtcgcg tcttatcagg cagcaggagg tcgattccgt tgaacgcgaa gactacggcg
       61 tcaaagcaac tatccggaaa acccgacaag tccgacgcgt ccgtgatgat gaattccacc
      121 gccggaaact tcgcgcggca agcttcgacc atcgcctctg cgtaatccac gccgacatag
      181 cgagacgccc ggctcgccag ataaggagta gtccgtccgc cgcctacccc aaggtcgagc
      241 accgcgcccc caactgggac ataggtctca aacagcaagc gctcacacgg ggtcaagtaa
      301 tccagagcgg catagtggga ggcaacttca ggggagttgt aggtctgaaa gtttgtttcc
      361 gcgctgagat gagtcatggc aggcctatac ttgcaccgtc ccaaccggca gcgcatctcg
      421 acctgcgcgc cgctgcagcc acgcgtggaa acccgatact tgtgcttcga attccgccag
      481 acccatgtcc gctgtcacat gcaaacgcgg cagcgcgtag ggcggcacat cggcccccaa
      541 cccaccgccg aaattcagga acgcggcagc gtagccggcc tcctgcgcca tagccagcac
      601 ctggggggtg acggactgag gatcgccgaa gggataggcg aacgcccaaa cttgccgctg
      661 gagaactgct tccagttgcg cccggctttc gctaatttct gttcgtgcca ggtcgggtgg
      721 cgcttgtgac agaactggat ggctcaacgt gtgcgcgcca atcgtcattc ccgccgccgc
      781 cagctgacaa agttcggtgc gggtcagcaa tccaaaccgg cgacaagata gcgaatcttc
      841 gccatcgaac ggctgccagg aactcgaacc cagttgcgta cgcgcaacgc tgagaaaaga
      901 tctacgggtt tcggattcga cccgggaaag ctgcttcaca tagttccacc agacggtcag
      961 gcgctgctcc cgttcccgca gttcgccgct cgacggaaag cctccgcccg agatctcgaa
     1021 cgaccccgcc ggagctcgca gaaacagcct catcaaatct tcgtaccaca gcgtatttct
     1081 tatctctgtg gtggaagccc cggttacgaa aaacaggcac cgtagtcttt cttgttgcaa
     1141 gacgggcaac atatccgtca ggcagttcaa aagcccatcg tcgcaggtca ggagaacggc
     1201 gcgcgccggc agctgaccac ggtcttcacg ccacgccagc aaatcctccg gagcaatcac
     1261 atcgtagtgt gccttgagca gctgcagttg ccggcgaagg gcttcagccg cgacgagatt
     1321 gccgtcaagc gagggatcga ccgattcgta acctttagga agcacgccat gataggtcac
     1381 caccgccagt ccgcgcgctc cgatgcgatg aaatgcgccc gccgcggaca acgaagggta
     1441 tagcacccgc ttcagaaagg ggctgacaat cttcattgac ggctgatcat atcccagctg
     1501 aagggagtgc cgcgttcaat atcacgggcg gcgtgctggc ccaggatctc agtcaggtgg
     1561 cgggtgtgaa gtccgtagcc ggggcggatc gagcggactt tttgcgcagt gaacacttcg
     1621 ccctgcttga tgtcgcacac tacgaacagc gagcgccgga acacccggct gctcgcttcg
     1681 tgcgggctga atccgaagtg aacctcgccc aaggctttct ccgcaacgcg cactgcatcg
     1741 accatcgccc ggaactcttc gggctcgagc gagaacgcac tgtccgggcc cggctccgag
     1801 cgcgataagg tcaagtgctt ctcgatgatg caggccccca gtgccaccgc ggccaccgga
     1861 actgcaatgc ccagcgtgtg atccgacagg cccaccggca caccgaagcg ccgcgccagt
     1921 tctggaatcg tggtcaggtt catctcctcg ggaggcgacg gatatgcact cgcacatttc
     1981 agcagcgcga tctgcgcggc acccgcctgt cgtgccgttc caaccgcttc ctcaatttcc
     2041 tcaaccgttg ccattccggt agagatgatc agcggtttgc ccgtttgcgc
//
LOCUS       DASPUL010000600         2349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_793600, whole genome shotgun sequence.
ACCESSION   DASPUL010000600 DASPUL010000000
VERSION     DASPUL010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793600"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VEJ00_13900"
     CDS             complement(<1..1290)
                     /locus_tag="VEJ00_13900"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HXX02303.1"
                     /translation="MIFRSKVNRQSRLVAALGAAALVGLLLGTANAQQTDPMDDLLDK
                     LKAKGVLSDDEYQTLKKAREDEQIELRQERRRQAQQAAQQAEKEQKQADEAAKKPKFD
                     ASPGIKSIQLFGDVRLRYESRVADSSFHVGPPPPATGSLPGGPDSETLDRWRYALRIG
                     IRGDLVEDWFYTLRFDTGTNPRSSWVTFGNNVSNAAGGQGPFGRGGDGIVVGQAYVGW
                     RATPWFTVQAGKMANPLFTTPMVWDPDINPEGIAERINYPVNDKLSLFANFGQFVYQN
                     FIPNSTSGGFGGISNDGYLFAWQGGVGYKFDAETNAKVGLTFYNYTGFPGTYLTGGGA
                     FTSSTVGGPNGFGGPFANGTLGAANSYPTNQAGLQFANGVNNLRYWELPVEVNFPLGG
                     ISAQVFGDVGYNIQADDRAQKGSFANLGSQAFAYQAGV"
     gene            complement(1609..>2349)
                     /locus_tag="VEJ00_13905"
     CDS             complement(1609..>2349)
                     /locus_tag="VEJ00_13905"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HXX02304.1"
                     /translation="RSGVEIFFTQVFRDGFFHADMHPGNILVDAHGRYIALDFGIMGT
                     LTDADKSYLARNFLAFFRRDYRRVAQAHLEAGWVPRDTRVDEFESAIRAVCEPIFDKP
                     LKEISFGRVLLRLFQTSRRFNVEIQPQLVLLQKTLLNIEGLGRELDPDLDLWKTAKPY
                     LERWMSEQVGWRAFARHMREEAPHWGVALPQLPRLLYRTLLGGDSDAIAAQLASAAAA
                     QKRQARWLIVLSALLAVLLVAQLIQLFV"
BASE COUNT          433 a          759 c          721 g          436 t
ORIGIN      
        1 gacccccgcc tgataggcaa aggcttgtga gcctaggttt gcgaagctgc ctttctgggc
       61 ccgatcatcg gcttggatgt tgtagcccac atcgccgaag acttgcgcac tgatcccgcc
      121 aagcgggaag ttgacctcca ccggcaactc ccaatagcgc agattgttca cgccgttggc
      181 aaattgcaat cccgcctggt tagtcggata gctgttcgcg gcgcctagcg taccgttcgc
      241 aaaggggccg ccgaagccgt tgggtccccc aacggtgctt gaggtgaagg caccaccccc
      301 cgttaggtag gtccctggga agcccgtata gttgtaaaag gtcagcccaa ccttcgcgtt
      361 ggtctccgca tcaaacttat aacccacccc gccttgccag gcgaacaagt agccgtcatt
      421 gctgatgccg ccaaagccgc cgctggtgga gttggggata aagttctgat agacaaactg
      481 gccgaagttg gcgaacaggc tcagtttgtc gttcaccggg tagttgatgc gctcggcgat
      541 gccctctggg ttgatgtccg gatcccagac catcggcgtg gtgaacagcg ggttggccat
      601 cttgcccgcc tgcacggtga accacggtgt ggcgcgccag cccacatagg cctggcccac
      661 aacgatccca tccccacctc tgccaaaggg cccttggcct ccggctgcgt tgctcacgtt
      721 gttcccgaag gtcacccagg acgagcgcgg gttggtgccg gtgtcaaagc gcaacgtgta
      781 gaaccagtcc tcgaccagat cgccgcggat gccgatgcgc agggcatagc gccagcggtc
      841 caaggtctcc gagtcaggac ctccggggag tgagccagtg gcaggtgggg gcggccctac
      901 atggaaactg gaatccgcaa cacggctctc gtagcgcagc cttacgtccc cgaatagctg
      961 gatgcttttg atccccgggc tcgcgtcgaa cttgggcttc ttggccgcct catcggcctg
     1021 cttctgctcc ttctcagcct gctgggcggc ctgctgggcc tggcgccgac gctcctgacg
     1081 cagttcgatc tgctcatctt cccgcgcttt cttgagcgtc tggtactcgt cgtcggatag
     1141 cacccccttc gccttcagct tgtcgagcag gtcgtccatc gggtcggtct gctgcgcatt
     1201 ggccgttccc agcaagagcc cgacgagagc cgcggcaccg agcgcggcca cgagtctact
     1261 ttggcggttg accttggatc ggaagatcat ttttttctct cctgatgaac caaaccgggc
     1321 tattcgcttc cgcccttgag caggcaacac accggcgcgg cgccaagtcg cgccgaggaa
     1381 cccgcacacc agcgtcgggg attctgcgga accactatga caatttgatg aacccgcgcg
     1441 gcctcggatc cctccgcggc gatggcggcg ccgtaacgtt cgccagcagc gaatgatccg
     1501 agcggcgcat ggctaaaagc gcgcatgccc cgtcgcgcgc cgaccttctg ctgcgaggat
     1561 caccgcccgt ccgccgggac tcgatcgcaa ggcagcgggt gccgcgggtc agacgaaaag
     1621 ctggatgagc tgcgctacga gcaagacggc gaggagagcc gacaaaacga tcagccaccg
     1681 cgcctgccgt ttttgcgcag cggccgcgct cgcgagctgc gcggcgatcg cgtcggaatc
     1741 gcctcccagc agcgtgcgat acagcagtcg cgggagttgg ggcaatgcca cgccccagtg
     1801 cggcgcttct tcacgcatgt gccgggcgaa tgcgcgccaa cctacctgct ccgacatcca
     1861 gcgctccaga tagggcttgg ctgtcttcca caggtcgagg tcgggatcga gctcacgacc
     1921 caggccttcg atgttgagca aagtcttctg cagcagcacc agctgcggct ggatttcgac
     1981 gttgaagcgc cgggaggtct gaaacaggcg cagcagcacg cgaccaaagg aaatctcttt
     2041 caggggcttg tcgaaaatcg gctcgcacac cgcccggatg gcgctctcga actcgtccac
     2101 gcgcgtgtcc ctcggaaccc agcccgcctc gagatgcgcc tgggccacgc gccgatagtc
     2161 gcggcgaaaa aacgcgagga agttgcgtgc gaggtagctc ttgtccgcat ccgtcagggt
     2221 gcccatgatc ccgaagtcga gtgcgatgta acgcccgtgc gcgtccacga gaatgttgcc
     2281 gggatgcatg tccgcatgaa agaagccgtc gcggaacacc tgcgtgaaga agatctcgac
     2341 accgctgcg
//
LOCUS       DASPUL010000601         2089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2035462, whole genome shotgun sequence.
ACCESSION   DASPUL010000601 DASPUL010000000
VERSION     DASPUL010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035462"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1224)
                     /locus_tag="VEJ00_13910"
     CDS             complement(<1..1224)
                     /locus_tag="VEJ00_13910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX02305.1"
                     /translation="MRSGQTPPTQPARLVLLLALGMFINYVDRGNLSTVGPVLQDQLH
                     LSSTELGSLFSAFFVTYVVGMVPGGWLADRFGAKRVLGVGAAIWSTATLLTGLAGSFI
                     SVLSLRLVLGVGETAAFPSISKVLATSIERTHIGLANGVIGCAYLIGPAVGTAFGGIL
                     LAHLGWRAVFIILGGLSLLWLAPWSRVVLAENGPPLTETTGAVPGFPQILRQRGLWGA
                     ALGSFAGSYGYYAMLSWLPTYLVKARGLSIESMTAIAASGFAINAISALLFGWAIDRW
                     VRSGHSPTMAWKVPMGLGSAAGIVATIGFAILPIHGCIGCLCLFMVLSGCTSVGYFAI
                     PQLLAGPGAAARFVGISNMSGNVAGIVAPLLTGVLVDASGAYWPAFVLIALVYVLGVV
                     GWVIVLPRVEPIRWAA"
     gene            1489..>2089
                     /locus_tag="VEJ00_13915"
     CDS             1489..>2089
                     /locus_tag="VEJ00_13915"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HXX02306.1"
                     /translation="MPANLSPEYKRAEQAFRMAREPRERLDCLKEMLRTIPKHKGTEH
                     LQGDIKSRIRELTEQIGGTHAGLAHRHSAHAVHQEGAAQICLTGPPNSGKSTLHAILT
                     GSRAEVGAFPGTTREPLPGMLHFEDIAFQLVDLPPISRGRMEPWMSGLLQTADAAWLV
                     VDLADPECAEQVLAVCGELAQRKITLTDHWPGCSGPDRAE"
BASE COUNT          464 a          646 c          638 g          341 t
ORIGIN      
        1 ggctgcccag cggatcggtt cgactctagg caggacgatc acccagccga ctacaccgag
       61 gacatagacc agggcgatca ggacgaaggc aggccaatat gcgccggatg catcgaccaa
      121 cacacccgtg aggaggggag caacaattcc ggcaacgttg ccagacatgt tcgagatgcc
      181 gacaaagcgg gctgccgcac cggggcctgc gagaagctgc ggtatggcaa agtaccccac
      241 agaggtgcag cccgagagaa ccataaacaa gcacaagcat cctatgcaac cgtggatcgg
      301 caggatcgca aagccgattg tggccacgat tccagctgcg ctcccgagtc ccatgggaac
      361 cttccaggcc atcgtgggcg agtgccccga ccgtacccac cggtcgattg cccatccgaa
      421 gaggagcgcg cttatggcgt tgatcgcaaa gcctgacgct gcaatcgccg tcatgctctc
      481 gatcgaaagg cctcgagcct tcaccaggta ggttggcaac catgaaagca tcgcgtagta
      541 cccgtagctg cctgcgaagg acccaagcgc tgcgccccac aatccccgtt ggcgcaggat
      601 ctgtggaaat cctgggacgg caccggtcgt ctcggtcagc ggcgggccat tctccgccag
      661 aacgactcgg ctccaagggg cgagccacag cagcgacagg cctccgagga tgatgaacac
      721 cgcgcgccaa cccagatgag ccaacaatat gccgccgaag gccgttccga cggccggccc
      781 gatcagatac gcgcagccga tcaccccatt ggccaaccca atgtgtgtgc gctcaatgct
      841 ggttgcgagg actttcgaga tgctcggaaa ggcggcagtc tccccgactc cgagtacgag
      901 ccgcagagac aatactgaga tgaagctgcc ggctaatccg gtgagcaagg ttgctgtaga
      961 ccaaatggct gccccgacac cgagtacacg cttggcgccg aagcggtcgg cgagccagcc
     1021 gccgggcacc atgcccacga cgtatgtaac gaagaacgca gaaaacagcg agcccagttc
     1081 agttgacgaa aggtgcaact gatcctgcag cacaggaccc acggttgaaa gattaccgcg
     1141 atcgacgtag ttgatgaaca ttcccagtgc caacagcagc acgagtcttg ctggctgcgt
     1201 gggcggagtc tgtccgcttc tcatagttat tcagaacact gcgccgccgc tagaatgcgc
     1261 caccaacgaa tggcaggcaa acccgtgcgc tccttccggg gcctaacggc cattagccgt
     1321 cgctcgtcac cgcgcgctga ctttccggaa atcggccgtt caagcttgcg ggctcgagaa
     1381 actttccgcg ccggagccca gggtgcggca cgtcgctgct gcgtgatgtc ggcgacctta
     1441 cgagtgacca taggtgcgcg gattgtccaa ggcgcttaaa ttatcgggat gcccgccaac
     1501 ctctctcccg aatacaagag ggccgagcag gccttccgca tggcacgcga accgcgcgag
     1561 cgcctcgatt gcctgaaaga gatgctgcgc acgattccaa aacacaaggg gacggagcat
     1621 ctgcaggggg acatcaagtc ccgcatccgg gaattgacgg agcagatcgg cggaacgcat
     1681 gcaggacttg cacaccgtca ttctgcgcac gcagtacatc aggaaggcgc ggcgcagata
     1741 tgcctgactg gtccgccaaa ttcgggcaaa tcgacgttgc atgcgattct gacggggtct
     1801 agggctgagg taggcgcttt tcctggaacg acgcgcgaac cgcttccggg gatgctccat
     1861 ttcgaggaca tcgcattcca gctcgtcgat ctgccgccga tctcgcgcgg ccgtatggaa
     1921 ccgtggatgt cgggtttgct gcaaacggct gacgcggctt ggctggtggt agatctcgcc
     1981 gaccctgagt gcgccgaaca ggtgctcgca gtctgcggcg agttggcgca acgcaagatt
     2041 actctcacgg accattggcc cggctgcagc ggcccggaca gggcagagg
//
LOCUS       DASPUL010000602         3150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1888645, whole genome shotgun sequence.
ACCESSION   DASPUL010000602 DASPUL010000000
VERSION     DASPUL010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1888645"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..494
                     /locus_tag="VEJ00_13920"
     CDS             210..494
                     /locus_tag="VEJ00_13920"
                     /inference="COORDINATES: protein motif:HMM:NF023159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXX02307.1"
                     /translation="MATQERRLQDLISVGPAMVRDFQILGVRSVSALARRNPEKLYEK
                     LCQLTGQAQDICCLDLFRAAVEQARNPRLPAEQCQWWYWSRRRKARDGRS"
     gene            481..1104
                     /locus_tag="VEJ00_13925"
     CDS             481..1104
                     /locus_tag="VEJ00_13925"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXX02308.1"
                     /translation="MAAAKTGKTGKKSGLTTPDLVLLSLLAERAMHGYQANLELERRQ
                     VRDWAGISRPQVYYSLEKLARLGLVRAAHTGEPSAGPERSVFETTTRGRSALADALER
                     AEWAMQRERPPFLTWIALSWQARPGVFEQQLQRRQNFLKKELEREEATLRAIRQEVGH
                     RFHEAVWAVGLTIQQFRTELRWARKLASEIRRRAPARHPQYADGDPE"
     gene            1168..1707
                     /locus_tag="VEJ00_13930"
     CDS             1168..1707
                     /locus_tag="VEJ00_13930"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HXX02309.1"
                     /translation="MSFSRPKKRSYTEDELYEYAVGALARRMRTVAELKRLMRARLED
                     ADSEYGQTLLELVIRRLKDQGYLNDSQYAASFSSLRRDNQKFGRLRVITDLKAKGVHG
                     ALIEKAVDAAFDGVSEEKQAREYLRKKRLNKPRDQKEAARVFRQLARAGFGTKTIFTI
                     LKKWEVDDETLSTLESESA"
     gene            1830..2216
                     /locus_tag="VEJ00_13935"
     CDS             1830..2216
                     /locus_tag="VEJ00_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02310.1"
                     /translation="MKASLAAILAAALLTAAPFVFAQDTSSTEKKSTGTEMKDATKTA
                     AKDTEKGAKTAAKGTEKGTKVAAKDTEKGTKVAAKDTEKGTKTAAKDTEKAAKKTGSA
                     MKKGVKAMEGKKDKPADQKPADAPKN"
     gene            2433..>3150
                     /locus_tag="VEJ00_13940"
     CDS             2433..>3150
                     /locus_tag="VEJ00_13940"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HXX02311.1"
                     /translation="MLTGSQVRRKFLDFFVQKGHKEVHSSSLVPANDPTLLFTNAGMN
                     QFKDVFLGLEKRDYVRATTSQKCVRAGGKHNDLENVGFTNRHHTFFEMLGNFSFGDYF
                     KKDAIAYAWELITSPQWFGISRDKLYVTIFEGAGPELGLIPRDGEAEEAWIRVGVPKN
                     RIFDGGVKDNFWQMGDTGPCGPCSEIHYDMGPAASDQGHTDCAFACDCGRYVEIWNLV
                     FMQFNRDASGKLNPLPRPSVD"
BASE COUNT          721 a          922 c          946 g          561 t
ORIGIN      
        1 gaacatcacc ggggtaattc tcttcacatt catagttttg tttttctcct cggccctggg
       61 ctttgccagg ggcaatacgg acgcgagcac ggccagagcc agaacaaaga ttgtgattcg
      121 catggaattt atccttgcca aacccatgta gtctaaatta gactagcaaa cgatactaga
      181 ctcacttcat cgtgtcaagg aggattttca tggcaaccca ggaacgccgt ctgcaggatc
      241 tgatctcggt aggtccggcc atggtgcgcg acttccagat acttggagta cgcagcgtat
      301 ctgcgctggc ccgccgcaac cccgagaagc tttacgaaaa actctgccag ctcacaggcc
      361 aggcgcagga catctgctgc ctcgacctat ttcgtgccgc ggtcgagcag gcgcggaatc
      421 cacggctgcc cgcagagcaa tgccagtggt ggtactggag ccgccggcga aaggcgcgcg
      481 atggccgcag ctaagaccgg caagacgggt aagaaaagcg ggttgaccac tccggacctg
      541 gttctgctca gccttctggc agagcgggcc atgcacggct accaggccaa cctggaactg
      601 gagcggcgcc aggtacgcga ctgggccggc atctcgcggc cccaggtcta ctattcgctc
      661 gagaagctgg cgagactggg cctggttcgc gcagctcaca ctggcgaacc gtcagcgggc
      721 ccggaacgca gcgttttcga gaccaccacc cgcgggcgct cggcattggc cgacgctctc
      781 gagcgcgcgg agtgggccat gcaacgcgag cggccgccct ttctcacctg gattgcgctg
      841 tcctggcagg ctcggcccgg ggtgttcgaa caacaactgc aacgccgcca gaatttccta
      901 aagaaagaac tcgagcggga ggaagccacg ctgcgcgcta tccggcagga ggttgggcat
      961 cgttttcacg aggccgtctg ggcggtcgga ttgacgatcc agcaatttcg caccgagttg
     1021 cgctgggcgc gcaagctggc gagcgagatt cgacgccggg cgccggcccg ccatccgcag
     1081 tatgcggatg gcgatccgga atgacgacta ccgtactcct ggaatccgtt cgccttcacg
     1141 ttccggttgc gctatcgtga gagcggcatg tcgttctctc gcccgaagaa gcgtagctac
     1201 accgaagacg agctctacga atacgcagtg ggcgcgctgg cgcgccgcat gcggacggtc
     1261 gccgaactca agcggctgat gcgggcgcgg ctggaagatg ccgattccga gtatggccag
     1321 acgctgctcg aactggtgat ccggcggctc aaagatcagg gatatctgaa cgattcgcaa
     1381 tacgcggcgt cgttctcctc cctgcgccgt gacaatcaga agttcggacg cctgcgagtc
     1441 atcaccgatt tgaaggccaa aggcgtgcac ggcgcgctga tcgagaaggc cgtcgatgcg
     1501 gccttcgacg gcgttagcga agagaagcag gcacgcgaat atcttcgcaa aaagcgcctg
     1561 aacaagccgc gcgatcaaaa ggaagcggcg cgggtcttcc gccagctggc gcgtgccggc
     1621 ttcggcacca agacgatctt caccatcctc aagaagtggg aagtcgacga cgagaccctg
     1681 agcacgctgg aaagcgaatc cgcgtagact cacaggaatc tcggcgccgt cactcggcat
     1741 ccagagaatc atgttgcggt gggtttgcgc tgaattaccc gctgttgcat actcactaca
     1801 ccattccgct acgacttgag gaggaagcta tgaaagctag cctggcagca attcttgcgg
     1861 ctgcattact gacagcagcc ccatttgtct tcgcgcagga caccagcagc acggagaaga
     1921 agtccaccgg caccgagatg aaagacgcga ccaagaccgc ggccaaggat accgaaaagg
     1981 gagcaaaaac cgcagccaag ggtactgaga aaggcacgaa agttgccgcc aaggacacgg
     2041 aaaagggaac caaggtcgcg gctaaggaca ccgagaaagg cacgaagaca gctgccaaag
     2101 atacagagaa ggcggcgaag aaaaccggca gcgccatgaa aaagggtgtg aaggcgatgg
     2161 aaggcaagaa agataagccc gccgatcaga agcctgctga cgcgccgaag aactagtcac
     2221 cacattcaac cacagacgac agtcggtcgt gttcaatcac cgccggcgtc atcccgaagg
     2281 cccgcgtttt cgctagaccc ccggctttag ccgggagctc gcaaagcgag cgggccgagg
     2341 gatctcgcgg ggatcgtacc tgccctcagc ggcgagacgg ccaatctctg tggatgactg
     2401 ccgctgtgtt cgttcgttta gaattaaagt tgatgttgac cggctcgcaa gtccgccgca
     2461 aattcctgga cttttttgtg cagaaagggc acaaggaggt gcattcgtcc tccctcgtcc
     2521 cagcgaacga tcccacgctg ctgttcacca acgcggggat gaaccagttc aaggatgtct
     2581 tcctgggcct ggaaaagcgc gattacgtgc gcgccaccac ttcgcagaag tgcgtccgcg
     2641 ccggcggcaa gcacaacgat ctcgaaaacg tcggcttcac caaccgccat cacacgtttt
     2701 tcgagatgct gggaaatttt tcgtttggcg actacttcaa gaaagacgcc atcgcctacg
     2761 cctgggagct gattacttcg ccgcaatggt tcgggatttc cagagacaag ctctatgtaa
     2821 cgatctttga gggagccggt ccggaactcg gacttatccc gcgagacggc gaagccgagg
     2881 aggcatggat cagagttgga gtgccgaaga atcgaatctt cgacggtgga gtaaaagaca
     2941 atttctggca gatgggcgac accggcccct gcggtccctg cagcgaaatt cactatgaca
     3001 tgggcccggc cgcgtccgat cagggacaca ccgattgcgc ctttgcctgc gactgcgggc
     3061 gctacgtcga aatctggaat cttgtcttca tgcagttcaa tcgcgacgcg agtggcaagc
     3121 tcaatccgct tccacgacca tccgtcgata
//
LOCUS       DASPUL010000603         2243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2035515, whole genome shotgun sequence.
ACCESSION   DASPUL010000603 DASPUL010000000
VERSION     DASPUL010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035515"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1109)
                     /locus_tag="VEJ00_13945"
     CDS             complement(156..1109)
                     /locus_tag="VEJ00_13945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HXX02312.1"
                     /translation="MITRLSGPEALAVQEAPDPVAKPGQSIVRVGAGGLNFADVMTTK
                     GGYPGTPAAPLIAGREFAGVEESSGRRVMGYTQWAAFAEKTATYSHMLWPVPDGWTDE
                     QGAAFPVNYFTAYLLYWQAGMIQPPAVGRTHRALIHAVAGGVGTAAVQIGKLLGVEMY
                     GTSSSEEKLMRVKPLGLQHAINYKQHDYEEVVKNLTHGEGVDAVFEMLGGEHTAKSTR
                     CLREFGRVIQYGTATGHAPQLDLRAMYAKSGMVQGLWLSYLSQRREIMEPAWKQLSAW
                     IAEGKLIPQIGHMFPMGRAVEAYKLLEEGRNYGKVVLKISS"
     gene            1455..>2243
                     /locus_tag="VEJ00_13950"
     CDS             1455..>2243
                     /locus_tag="VEJ00_13950"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HXX02313.1"
                     /translation="MDIIFVRLLFVVVVAVTCFVIKPFGQNSTVDAGIGTLIGTAIVL
                     FEWKLRTVSLKRLIGAAIGSILGICGAYLFALVIRSSVPAGNTQSFLQIMVMLLMAYV
                     GLIVGANKGDLLNLSALGGIFGGEKQAKKSYKILDTSVIIDGRIADIAETGFLDGVIV
                     TPQFVLRELQLVADSADSLKRNRGRRGLDILQRLQKVANLQIQIVEDDFPAIREVDLK
                     LIELAKMYEGKIITNDFNLNKVAQLQGVEVLNINELANSLKPIVL"
BASE COUNT          425 a          681 c          662 g          475 t
ORIGIN      
        1 ccaccccaac gtgggagtca tctccaacat cgattccacc caccctgctc cgaccttctt
       61 cgataacaac ggcggccgcg ccttcgccct gcgcatcatg ctgctgggga aagacaagtg
      121 agtgtgttct cgctgccagg ccctgtaaac ggcgtttaac tggaaatctt cagcaccact
      181 ttcccgtagt tccttccctc ctcgagcagc ttgtaggctt cgacggcgcg gcccatcgga
      241 aacatatgac cgatctgcgg aatcagcttg ccttcggcga tccatgccga gagctgcttc
      301 cacgccggct ccatgatctc gcgcctttgc gacaggtaag acagccagag cccttgcacc
      361 atgccggatt tggcatacat ggcgcgcagg tcaagctggg gagcatgccc cgtcgccgtg
      421 ccgtactgaa tgacgcggcc aaattcgcgc aggcagcgcg tgctcttggc tgtatgctcg
      481 ccgcccagca tttcaaatac ggcgtcgacg ccttcgccgt gcgtgaggtt tttcacgacc
      541 tcctcgtaat cgtgctgctt gtaattgatg gcgtgctgca gaccgagtgg cttcacgcgc
      601 atcagtttct cttccgacga tgacgtgcca tacatttcca cgccgagcag tttcccgatc
      661 tgcaccgccg cggtgccgac cccgccggcg acggcgtgga tcaaagcacg atgcgtccgc
      721 ccaaccgcag gcggctgaat catgcccgcc tgccagtaca ggaggtaagc cgtgaagtag
      781 ttaaccggga aggcggcgcc ttgctcatcg gtccagccgt cagggacggg ccagagcatg
      841 tgcgaatagg tggcggtctt ctcggcgaag gcagcccact gggtgtagcc catcacgcgg
      901 cgtccggagc tttcctctac gccggcgaat tcacgccctg cgatcagcgg cgcggctggc
      961 gtgccgggat agcccccctt ggtggtcatg acatcggcga aattgagacc cccggcgccc
     1021 acgcggacaa tgctctgtcc gggcttcgcc accggatccg gtgcctcctg gaccgcaagg
     1081 gcttcgggac cggacaggcg agtaatcatc aaggctttca tggagacaca gtgtagcagg
     1141 agcctttgga ctccggtttc ggcgctcgga atctagaagt ggagaccgga tgtcggcacg
     1201 atctgcggtt tataggctcg ttcgtgccat caggtttcct cgccgctatg tatcagcggg
     1261 tatatacttt gggcgtcgga ttcctgatta cccggccgag ccctacgggc cgggacgggg
     1321 cgctggtttc attcccttat tcatgggcgc tccgtatgcg aaaggctgac tcccgccggg
     1381 gcggcgcgga ggaggcacgg gtgcaattag acctggtagc tgccttccag cgattgcatc
     1441 cgggggaggg tgctgtggac ataatctttg tacgccttct tttcgttgtg gttgttgcgg
     1501 ttacctgctt cgtcatcaag ccctttggac aaaacagcac ggtcgatgcc ggtatcggca
     1561 cgctgattgg gacggccatc gtgctcttcg aatggaagct gaggacggtc agcctgaaac
     1621 gtctcatcgg cgcggccatc ggcagtatcc tcggcatttg cggcgcctac ctgttcgctc
     1681 tcgtcattcg cagcagcgtc cccgctggaa acacgcagag cttcctgcag atcatggtca
     1741 tgctgctgat ggcctacgtt ggcctgatcg tcggcgccaa caagggtgac ctgttgaacc
     1801 tctccgctct gggtggaatt ttcgggggtg aaaaacaggc caagaaaagc tacaagatcc
     1861 tcgacaccag cgtcattatc gacgggcgca tagccgacat cgctgagacc gggttcctgg
     1921 atggcgtgat cgtcaccccg cagtttgtgc tgcgcgaact gcagctggtg gccgactcgg
     1981 ccgattcgct gaaacgcaat cgtgggcgcc gcggcctcga catcctgcag cgcttgcaga
     2041 aggtcgccaa cctgcaaatt cagattgtgg aagacgactt cccagcgatc cgcgaagtcg
     2101 acctgaagct gatcgagctg gcaaagatgt atgagggcaa gatcatcacc aatgatttca
     2161 acctcaacaa ggttgctcag ttgcaggggg ttgaagtctt gaacatcaac gaactcgcga
     2221 attcgctcaa acccatcgta ctg
//
LOCUS       DASPUL010000604         4739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1614487, whole genome shotgun sequence.
ACCESSION   DASPUL010000604 DASPUL010000000
VERSION     DASPUL010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4739)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4739
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614487"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="VEJ00_13955"
     CDS             complement(<1..947)
                     /locus_tag="VEJ00_13955"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXX02314.1"
                     /translation="MTLSALLVSVDDNATQILRRVLEELNIGVEFCPDFARATMRLAQ
                     ERFDLVIVDGESNSDVVSLLRETRLSRLNDATLAVVVVSGQESIRELFSLGVNFALYK
                     PVAHERALSSLRAARAVMRKEKRKSARATVHAHATVEYANVEQEKATLVNLAADGMSV
                     RFGKKFPPTGKVYFQFRLPGQSASIRLSGQVIWQDWNGRAGIHFVDVPKASRKALSDF
                     LNSSPLQDSQREQTPDVSMPELGGQEVTVEMELPLPVAAASGAEPTRGSEKTKRAAAE
                     TAMQALSNVHAEARADADNRRTQTRYSCRLGAEVYRTGTS"
     gene            1289..1906
                     /locus_tag="VEJ00_13960"
     CDS             1289..1906
                     /locus_tag="VEJ00_13960"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXX02315.1"
                     /translation="MTSSKSRGFSLLELMIVVLIGFTVASMSVMALMPLFNRAHMDTA
                     YDTTLMAMRNARHLAITQSHQYYVNFIPAAGAVPPQIQITYQPPAAVVGGILPPVQQV
                     MVYSLPTDINFAVMAGMPATAPDGFGTGLVPIDFGQGLGAGSLNYVCFMPDGSAQDNL
                     GNFNSGIIYMVRPADTVYSARAITVWGATGRMRGWRLYPSGWVQQ"
     gene            1903..2529
                     /locus_tag="VEJ00_13965"
     CDS             1903..2529
                     /locus_tag="VEJ00_13965"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXX02316.1"
                     /translation="MIRYKTANAGVRNAASGFSLIEVMIAIVVMSVALLALIAAFGAA
                     VAATQSAQEDLIARQKALEAIESIYTARNSQQIPFSAVANTANGGIFLPGALPLLCAG
                     PDGLVGTADDVKCTTAAGAVCPDGGAECMVLPGPDGILGTPDDVTMSLGNFTRTITLT
                     TVQYPPGAGPLAGQTNPNMMAISITVSYTKAGLPARSFTANGLITSYH"
     gene            2565..3767
                     /locus_tag="VEJ00_13970"
     CDS             2565..3767
                     /locus_tag="VEJ00_13970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXX02317.1"
                     /translation="MRTAKTQRGFSLLELLVASAIGLVAMAAMTSLFKTGMDATFTVA
                     QRAETQQNMRAAIELMTKDITLAGAGLPSGGLQLVSGGTLSNIACNQGGTCYVNGGTY
                     PPSAAGAPNFMYPIEPGFNTGVQGGAAIVAAPGLTNSRLTTVYCDYNFPMSNFGFTSN
                     FPGAATVTVNAIAAPAVGLPTNIQAAGGLQVGDVLLFVINTPGNGKTVTGTSLVQNAA
                     VVGEITGIAGNPAPPAVTAGAVVAFAPGDALNFNQNGGGQTNNMASVAAAANAPGAQM
                     SVCRLEVVTYFLQVPPAGGGLVQTPRLMRQVNGLNAVPVADNIINLQFTYDVINSATG
                     TVNSNQQDPIGAALSPALIQKVNIVIMGQSLTTKGNKAQSMYLATAVSTRDMSFCNSY
                     SSNTTQCQ"
     gene            3795..>4739
                     /locus_tag="VEJ00_13975"
     CDS             3795..>4739
                     /locus_tag="VEJ00_13975"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HXX02318.1"
                     /translation="MGTSQRNSRGFTLIAALLLTVLLSGLAVGLLFMVSNEARMGGND
                     LEGNLAYYAAEAGIENLTSEISQLYQTSQTPNAAAINALWQNSSNYVTAVPGANINNI
                     TYLPQITWPATQTDGTPCANAPDPCGSWDIVGSGPDQGMVATLIPFTIQVTATRQAGT
                     GQSGSQSLLAASGASVNLTRTVEVALLPAFEFGVFCDGDCDYFAGPNFNFGGRVHTNG
                     NLFLASGSALTFTDKIAAVGQVVMDQLENGHPTSVNYTGQVYVPSAAGACPPAPGFGP
                     VAPSCNQLPFGSWTGGFPPTGTANTTTWPPESKSTFAGF"
BASE COUNT          928 a         1474 c         1448 g          889 t
ORIGIN      
        1 gaagtgccgg tgcggtagac ctccgcgccc agccggcagg agtaacgagt ctgggttctg
       61 cgattatcgg catccgcgcg cgcttccgcg tgaacgttgg agagagcctg catagcggtt
      121 tctgccgctg cgcgcttcgt cttctcgctt ccgcgggttg gttctgcccc cgacgccgcg
      181 gctactggca gaggcaattc catttcgacg gtcacttcct gcccacccaa ctcgggcatg
      241 ctgacatctg gggtctgctc gcgctgcgaa tcttgcagcg ggcttgagtt cagaaagtcg
      301 ctgagcgcct ttcgcgatgc cttgggaacg tccacgaagt ggatgccggc gcggccgttc
      361 caatcctgcc agatcacctg tcccgagagg cgaatgctcg ccgactgccc gggcagccgg
      421 aactgaaaat acaccttgcc cgtaggcgga aacttcttgc cgaagcggac cgacatgccg
      481 tccgcggcca ggttcacgag ggtcgccttc tcctgctcga cgttggcgta ttccacggtg
      541 gcgtgagcat gcacggtggc gcgcgcgctc ttgcgctttt ccttccgcat cacggcgcga
      601 gccgcgcgga ggctgctcag cgcgcgttcg tgagcgaccg gcttgtagag agcgaaattg
      661 acgcccaggg aaaacaactc tcgaatgctc tcttgacccg acacaaccac cactgccagg
      721 gtcgcgtcgt tcaggcgcga caagcgggtc tcacgcaaca gggagactac atccgagttt
      781 gattcgccat cgacgatcac caggtcgaag cgctcttgcg ccaggcgcat cgtggcccgc
      841 gcgaaatcag gacagaactc aactccaatg ttgagctctt ccaggacccg gcgcaaaatc
      901 tgggtcgcat tgtcgtcaac tgacaccaac agggcagata gagtcatagc tttgccccat
      961 cctagagttc cggcggtgct gcccacaagt taccagggtc acggcgaaac tgggggtagc
     1021 gccgtaagtg gttggatttc tctcatgact ctagtaccca agcgggataa caaaaggcca
     1081 atcttcgggc attgcaccgt cgtgctttga tttcacttcc agcgcttcct aggatgaaca
     1141 gggacgcgat ccacggtcgc gccacatctg tgtttattgg tccgagagcg gaaaagctcg
     1201 gcgtagtcct ccctgaaacg tgcatcgtga gctcaaccgg gagatgcgga tccggcaagg
     1261 cggccgctct cgccacgaaa gaaactttat gacttcgagc aagagccggg gattttcgct
     1321 gctggagttg atgatcgtcg tgctcatcgg cttcacggtg gcgtcgatga gcgtgatggc
     1381 actgatgccg ctgttcaacc gggcgcacat ggacaccgcg tacgacacga cgctgatggc
     1441 gatgcgcaat gcgcgacacc ttgccatcac ccagagccac cagtactacg tgaacttcat
     1501 ccctgcggcc ggcgcagtgc cccctcaaat ccagatcacg taccagccgc ctgcggcggt
     1561 ggtcggcggc atcttgcccc cggtgcaaca ggtgatggtg tattcgctgc caacagacat
     1621 caactttgcc gtgatggccg gcatgccggc gacggcgccc gatggcttcg ggaccgggct
     1681 ggtgccgatc gatttcgggc aaggcctggg cgcgggcagt ctgaactacg tctgcttcat
     1741 gcccgatggc tcggcacagg acaacctggg gaacttcaac agcggaatca tctacatggt
     1801 acggcctgcc gacactgtct atagcgcgcg cgcgatcaca gtgtgggggg ctaccggccg
     1861 gatgcgcggc tggcgtttgt atccctcggg atgggtgcaa caatgattcg atacaagacg
     1921 gcaaatgcag gcgtgcgaaa cgcggccagc ggtttctcac tgattgaagt gatgatcgcc
     1981 atcgtggtga tgtcggttgc cctgctggcc ctgatcgcgg cgtttggcgc ggcggttgcg
     2041 gccacccaat ccgcccagga agacctcatc gcgcggcaga aggcgctgga agccatagag
     2101 agcatctaca ccgcgcgcaa cagccagcag attcccttta gcgcggtggc gaacaccgcc
     2161 aacgggggga tcttcctgcc cggagcgctg cccctgctgt gtgccgggcc cgatggcctg
     2221 gtgggaacgg cggacgatgt gaagtgcacg acagccgccg gagcggtttg cccggatggc
     2281 ggagccgaat gtatggtgct gcccggcccg gacggcatcc tgggcacgcc cgacgacgtc
     2341 accatgagcc tgggtaattt tacccgcacc atcacattga ccacggtgca gtacccgccg
     2401 ggagccggtc cgttggccgg tcaaacgaat cccaacatga tggcaatcag catcacggtg
     2461 agctatacga aagcgggatt gcccgcgcgc tcattcaccg cgaatggatt gatcaccagc
     2521 tatcactgat gcggacagga atggcccata cgaggatctt gactatgcga acggcaaaaa
     2581 cgcaacgcgg tttcagcttg ctggagttgc tcgtggcttc ggccatcggc ctggtcgcca
     2641 tggcggcaat gaccagcctg ttcaagaccg gcatggatgc gaccttcacg gtcgctcagc
     2701 gggcggagac gcaacagaac atgcgcgccg ccatcgaact gatgaccaaa gacatcaccc
     2761 tggccggagc aggactgccg tcgggcgggc tgcagctggt ttcggggggc actctgtcga
     2821 acatcgcctg caaccagggc ggaacctgct acgtgaatgg cggaacgtat cctccgagcg
     2881 cggctggcgc ccccaatttc atgtatccca tcgagcccgg cttcaatacc ggagtccagg
     2941 gtggcgcggc gatcgttgct gccccgggcc tgaccaacag ccggctcacc acggtctatt
     3001 gcgattacaa ctttccgatg tcgaatttcg gcttcacatc caactttccc ggagcggcca
     3061 cggtgacggt gaacgccatc gcggccccgg ccgtgggatt gccgaccaat atccaggccg
     3121 cgggcggact gcaagttggc gacgtgctcc tgttcgtcat caatacgccg ggtaacggga
     3181 aaaccgtcac cggaacgagt cttgtgcaaa acgccgcagt agtgggagag atcacgggga
     3241 tcgccggcaa cccggcgcct cctgctgtaa ccgcgggcgc cgtcgtggcc tttgcccccg
     3301 gcgatgcact caatttcaac cagaacggtg gcggccagac caacaacatg gctagcgtag
     3361 ccgcagcggc caacgccccg ggagcgcaga tgagcgtttg ccggctggaa gtggtgactt
     3421 acttcctgca agttccgccc gccggcgggg gtttggtcca gaccccgcgg ctcatgcgcc
     3481 aggtcaatgg gctgaatgcc gtgccggtag ccgacaacat cattaatttg cagttcacct
     3541 acgacgttat caattctgcc accgggacag tgaactccaa ccagcaggat ccgattggtg
     3601 cggcgttgtc ccccgcgctg attcagaagg taaacatcgt gattatgggt cagagcctga
     3661 cgaccaaggg caacaaagca cagagcatgt acctggcaac ggcggtcagc acgagagata
     3721 tgagcttctg caattcgtac agttccaata ccacgcaatg ccagtgagca ccgagtttgc
     3781 ttgaggggag aaccatggga acgagtcaac gaaattcgcg agggttcacg ctgattgcag
     3841 ccctgctgct gacggtcctg ctgtcgggac tggccgtggg actgctcttc atggtgagca
     3901 atgaagcgcg catgggcggc aacgatctgg aggggaatct ggcgtactac gccgccgagg
     3961 ccggcatcga gaacctgacc tcggagatct cccagcttta ccagacttcg cagacaccga
     4021 acgccgccgc catcaatgcg ttgtggcaga actcgtcgaa ctacgtgacg gccgttcctg
     4081 gagcgaacat caacaacatc acctacctgc cgcagatcac ctggccagcg acgcagaccg
     4141 acggaacccc ctgcgcgaac gctccggatc cgtgtggctc ctgggacatc gtggggtcag
     4201 gaccggacca gggcatggtc gcgaccttga ttcccttcac cattcaggta acagcaaccc
     4261 gccaggccgg cacaggtcaa agcggctcgc agtcgctcct ggcggcgtcg ggcgcttcgg
     4321 tgaacctgac gcgcactgtc gaggttgctt tgctgcccgc gtttgagttc ggcgtcttct
     4381 gcgatggcga ctgcgactac tttgccggcc cgaactttaa ctttggcgga cgggttcaca
     4441 ccaatggcaa cctgttcctg gcttccggca gcgcgttgac tttcaccgac aagattgcag
     4501 ccgtcggcca agtcgtaatg gatcagctgg agaacggcca ccccacgtcg gtgaactaca
     4561 cgggccaagt ctatgtgccc agtgccgccg gcgcctgtcc gcccgccccg ggtttcggac
     4621 cggtagcgcc gagctgcaat cagcttcctt ttggcagctg gaccggcggc tttccgccga
     4681 ccggcaccgc gaacactacg acctggccgc ctgaatccaa atcgacgttc gccggattc
//
LOCUS       DASPUL010000605         5353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_113377, whole genome shotgun sequence.
ACCESSION   DASPUL010000605 DASPUL010000000
VERSION     DASPUL010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113377"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VEJ00_13980"
     CDS             <1..517
                     /locus_tag="VEJ00_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02319.1"
                     /translation="TFDADEPEALVGVAQVLAERTRDTDLDKDQRLAEAKKDAERALV
                     TIDTDVPASGYPPEQLNAYKNFMRSEAYAILGTLAFNAKNYPEAETNLRKSIDVFPQQ
                     PDAIAVFRLAVALDYENKVPEAMKYIDQAVDLTKDHPDSGVGKAARDEKDRLAKLSGG
                     TAPGTTPPPKN"
     gene            604..1461
                     /gene="rnc"
                     /locus_tag="VEJ00_13985"
     CDS             604..1461
                     /gene="rnc"
                     /locus_tag="VEJ00_13985"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HXX02320.1"
                     /translation="MRVRVVKASILPTSCAHDSMKEREITALEAGLGYHFRHRAVLEQ
                     AVTHSSQAREQESQRAGEGKYRVSDNEQLEFLGDSVLSLVTSEELFQRFPHFREGELS
                     KLRAHLVSEKHLIRVAQQLELGHYLRLGRGEEKSGGRSKTALLVDALEAILAAMYLDG
                     GLEVAREFVRRHVILPELERMERGGGMLPVTDFKSALQEAVQSLGLPQPAYVLVEEAG
                     PEHSKTFTVEARLLAKNNKDPEFVGRAQGSTKKTAEQDAARQLLAFLASRSKATDTKA
                     GGGSQLPGR"
     gene            1540..2265
                     /gene="lepB"
                     /locus_tag="VEJ00_13990"
     CDS             1540..2265
                     /gene="lepB"
                     /locus_tag="VEJ00_13990"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXX02321.1"
                     /translation="MIGSLQSLVGTIVIAIFVITFIVQAFQIPSESMEKTLLVGDYLL
                     VNKLCYGARGLGDAVMPYQRIERGDIIVFHYPVDPTQHFVKRVIGVPGDRLRLISKKV
                     WINGKPLEEPYVHFLEPPNNRDPGSMFRDNFPRTDVSVLSIDGKWWQEIHKLVEDGQL
                     IIPEGHYFVMGDNRDDSQDSRYWGFVPRENIVGRPLVIYWSVQQWDRDGSTSFTRRLY
                     HIAYAVTHFFQITRWNRTLRLVH"
     gene            2367..3152
                     /gene="lepB"
                     /locus_tag="VEJ00_13995"
     CDS             2367..3152
                     /gene="lepB"
                     /locus_tag="VEJ00_13995"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXX02322.1"
                     /translation="MAKKENPVEAKTEEKPKETTVEFMASLAAVLVTGLFIITFVVQT
                     FVIPSSSMEHTLEIGDHVLVNRVQFAPKTSWLGPLLPYRDVRRGDIVVFLHPDPQQAG
                     IYVVKRIIGIPGDHIRLRDGVVYRNGEALNEPYILHDRDNLNDIYRMNFPSIPPMEGE
                     GLSVDWLVDLPSNIKNGELIVPPGKYFAMGDHRGVSLDSRYWGFIPKQNIIGRPLFIY
                     WSFAAPESDYTSTSWGDRILSLAKTILHFVDKTRWSRTLRLVR"
     gene            3176..>5353
                     /locus_tag="VEJ00_14000"
     CDS             3176..>5353
                     /locus_tag="VEJ00_14000"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXX02323.1"
                     /translation="MKLFLSRRRAIVAAAVILLLLFLLRPGASSLKARIISSISAAVG
                     RSVDMSSVQLRLLPRPGFDLNNLVVYDDPAFGAEPILRASEVTADLRLTSLLRGRLEI
                     ARLDLTEPSLNLVRGQNGRWNVEALLERTAHTPLAPTAKAKSEPRPGFPYIEGSSARI
                     NFKSGLEKKPYALTNTDFSLWQESENEWGLRLKAQPFRSDLNLNDTGVLQVDGTWQRA
                     SSLRDTPLQFSVEWSRAQLGQVTKFLTGNDLGWRGGAQLDVKFEGTPAHLHAISDARV
                     QDFRRYDITRGEPLPLAAHCDGQYSSPDHAFHELVCIAPVGNGLITLKGDVGLPGSHR
                     YGLVLTAENVSAGPAVALLQRLKKNLPDDLAAAGSLRGSVFIHEDAAAESPLQIEGRG
                     EVSDLRLVSAIDKAEIGPEMLPLVVTTGDSISAEHGRRTSHGRPAVPQAPNGLRIEFG
                     PFPVAMGHGGSPTAQGWINRTGYGIAVAGEADVAKALRTARMLGLPAIATTAEGDAQL
                     DLQIAGAWGNGATAPSTASPVTGTVKLHNVRVAMHGVGAPVEISSAEMQLSNEKVQIA
                     KLSAEAAGAAWNGSIEMPRGCGSPDACQVHFNLNASQIVLGELKEWVSPRPAEQPWYR
                     VLTKAAPSAPSFLSSLRARGRVTAERLVIRNVPLTHVAANLSLESGNLKLSDLTAEVL
                     GGKHQGEWAADFSATPAPCHGSGTLTGIALSQAADLMKDSWISG"
BASE COUNT         1098 a         1481 c         1774 g         1000 t
ORIGIN      
        1 gaccttcgac gccgatgaac cggaggcgct ggtcggagta gcacaagttt tggccgagcg
       61 gacgcgggac acggatcttg acaaggacca acggctggcc gaggccaaga aggacgcaga
      121 gcgggcattg gttacgatcg acaccgacgt tccggcctcg ggctatccgc cggagcagct
      181 caacgcgtac aagaacttca tgcggtccga ggcgtacgcg attctgggga cgctcgcctt
      241 caatgccaag aactatccgg aggcggaaac gaatctgcgg aagtcgatcg acgtgttccc
      301 gcagcagccg gatgcgatcg ccgtattccg cctcgcggtc gccctggact acgagaacaa
      361 ggttcctgag gcgatgaagt atatcgatca ggctgtggac ctcaccaagg accatcctga
      421 tagcggcgtg ggcaaggccg cgcgcgatga gaaggatcgg ctggccaagc tcagcggagg
      481 cacggccccg gggacgacgc cgcctccgaa gaactaaggc tgcaatggca gaaacctaga
      541 aacgcccaga acgccggggc tcgccaaggg cgcggaaaaa ccggagctta gctgagtccc
      601 gctttgcgcg tacgcgtggt gaaggcatcg attttgccca ccagctgtgc tcacgacagt
      661 atgaaggaac gggaaattac ggcgctggaa gcagggctgg gctatcactt tcggcatcgt
      721 gcagttcttg agcaggcagt cacccacagc tcgcaggcgc gggagcagga atcgcagcgg
      781 gcgggcgagg gcaagtaccg ggtcagtgac aacgagcaac tggagttcct gggagactcg
      841 gtgctgtcgc tggtgaccag cgaggaattg ttccagcgct ttccgcactt ccgcgaagga
      901 gaactttcga agctgcgggc gcacctggtc agcgagaagc atctaatccg ggtagcgcag
      961 cagttggaac tgggccacta cctgcgcctg gggcgaggcg aggaaaagag cggaggccgc
     1021 agcaagacgg ctctgctggt ggatgcgctg gaggcgattc tggccgccat gtatctggat
     1081 ggggggctgg aggtggcgcg cgagttcgtg cggcgccatg tgatcctgcc cgaactggag
     1141 cggatggagc gcggaggcgg catgcttccg gtgaccgact tcaagtcggc gctccaggaa
     1201 gcggtgcaaa gcctgggctt gccgcagccg gcgtatgtgc tggtggaaga ggccgggccg
     1261 gagcacagta aaacgtttac ggttgaggcc cgcttgctgg cgaagaacaa caaagatccg
     1321 gagttcgtgg gacgagcgca aggctcgacc aaaaagacag ccgaacagga tgcggcgcgg
     1381 caactgctgg ccttcctggc ttcgcgatcg aaggcgactg ataccaaggc ggggggcggg
     1441 agccagctgc ccggcagata agtggagtcc atggaatccc catctttacc agttgaacga
     1501 cctgccgccc ggcctttggt aagcacggtc ggatcggatg tgattggctc gctgcagtcg
     1561 ctcgtcggca ctattgtcat cgcgattttc gtcattacct ttattgtgca ggccttccag
     1621 attccctcgg agtcgatgga aaaaactctg ctggtcggcg actacctgct ggtcaacaag
     1681 ctgtgttacg gcgcccgcgg gttgggcgat gcagtgatgc cgtatcagag gatcgagcgc
     1741 ggggatatca tcgtgtttca ctacccggtg gatccgacac agcactttgt gaagcgtgtg
     1801 atcggagtgc cgggtgaccg tctgcggttg atcagcaaga aggtctggat caacggcaag
     1861 ccgctggagg agccgtacgt gcactttctg gaaccgccca acaatcgcga ccccggcagc
     1921 atgttccgcg acaacttccc gcgcacagat gtttcggtgc tcagcatcga cgggaagtgg
     1981 tggcaggaga tccacaaact ggtcgaggac ggccaactca ttattcccga ggggcactat
     2041 ttcgtgatgg gagacaaccg cgatgacagc caggacagcc gctactgggg gtttgtgccg
     2101 cgggaaaata ttgtcgggcg gccgctggtg atctactggt cggtgcagca gtgggaccgg
     2161 gatgggtcga cttccttcac gcgcaggttg tatcacattg catatgccgt gacgcacttc
     2221 ttccagatca cgcgatggaa tcgcactctg cggctggtac actgaagatc tggtgcaggc
     2281 agagcctgaa ggcgccaatc atgcggggca gcatgcctga aggcaagccc tgattatcag
     2341 tcaatagact tcacggaagc gcgatcgtgg caaaaaaaga gaatcccgta gaagcgaaaa
     2401 cggaagagaa gccgaaggag acgacggttg agttcatggc ctcgctcgcc gcggtgctgg
     2461 tgaccggact gttcatcatc accttcgtcg tgcagacctt tgtgattcca tcgagctcga
     2521 tggaacacac gcttgagatc ggcgatcacg tgcttgtgaa tcgcgtccag ttcgcaccga
     2581 agacctcgtg gctgggtcca ctgcttccct atcgcgacgt gcggcgggga gacatcgtgg
     2641 tgttcctgca tccggatccg cagcaggccg gcatttacgt ggtgaagcgc atcatcggca
     2701 ttccgggaga ccacatccgt ttgcgcgacg gcgtggttta ccgcaatggc gaggcgctga
     2761 acgaacccta catcctccac gacagggaca acctcaacga catctaccgg atgaattttc
     2821 catcgatccc gccgatggag ggcgaaggcc tatcagtcga ctggctggtg gatttgccgt
     2881 cgaacatcaa gaacggcgag ctgatcgtgc ctccgggcaa gtacttcgcc atgggcgacc
     2941 accgcggcgt cagcctggac agccgctact ggggattcat tccgaagcaa aacattattg
     3001 gccggcccct gttcatctac tggtcgttcg ctgcgccgga gagtgattac acgagtacga
     3061 gttggggaga ccgtatcctg tccctggcga aaacgatcct gcacttcgtc gacaagacgc
     3121 gctggtcgcg gacgttgcgg cttgtgcgat aggtccgcgt gaaggtgagc ccgcagtgaa
     3181 gctcttcctc tccagaaggc gcgcaattgt ggcggcggca gtgattctgc tgctgctctt
     3241 ccttctccgc ccgggcgctt cgagtctgaa ggcccggatt atttcctcga tcagcgcagc
     3301 tgtcgggcgg tccgtggaca tgagctcggt tcagctgcgt ctgctgccaa gaccgggatt
     3361 cgacctcaac aatctcgtgg tgtacgacga tcccgcgttc ggagcagagc cgatcctgcg
     3421 ggcgagtgaa gtaaccgcgg atctgcggct gacttccctg ctgcgcggac ggctggagat
     3481 cgcgcggctc gatctgactg agcccagcct gaatcttgtc cgcggacaga acgggcgctg
     3541 gaatgtggaa gcgctgctgg aacgaacagc tcacacccca ctggcgccca ccgcgaaggc
     3601 gaaatcagag ccgcggccgg ggtttcccta catcgaaggc tcgtcggcgc gaattaactt
     3661 caagagcggt ctggaaaaga agccctacgc gctgaccaat accgactttt ccttgtggca
     3721 ggagtccgaa aatgagtggg gcttgcgact taaggcccag ccttttcgca gcgatttgaa
     3781 tttgaacgac accggcgtgc tgcaggtgga cggaacatgg cagcgggcgt cctcgctgcg
     3841 cgacacgccg ttgcagttca gcgtggagtg gagccgcgcc cagttggggc aggtcacgaa
     3901 gtttttgacg ggcaacgatc ttggctggcg cggaggtgcg cagctcgatg tgaagttcga
     3961 gggaacgccg gcgcaccttc atgccatcag tgacgccagg gtacaggact ttcgccgcta
     4021 cgacattacg cgcggcgagc ctctgcctct ggccgcgcat tgcgacgggc aatacagctc
     4081 gcctgatcac gcctttcatg aactggtgtg catcgctccg gtgggaaatg gactgatcac
     4141 actgaaaggt gacgtgggtc ttcccggaag ccatcggtac gggctggttt tgactgcaga
     4201 gaatgtctcc gccggccccg ccgtggctct gctgcagcgg ttaaagaaaa atctgccgga
     4261 tgacctggca gccgcaggct cgcttcgtgg aagcgtcttc attcatgagg atgcggcggc
     4321 ggaatcgcca ctgcaaatcg aggggcgggg agaagtctcg gatctgcggc ttgtttcggc
     4381 gatcgataag gccgaaattg gaccggagat gctgcctttg gtagtgacca ccggtgattc
     4441 catatccgcg gagcatgggc gccgaacatc gcatggccgc cctgcggtgc cgcaggctcc
     4501 aaacggtttg aggatcgaat ttggaccttt tcctgttgcc atggggcacg gagggtctcc
     4561 aactgcgcag ggctggatca accgaactgg ctacgggatc gcggttgcgg gggaggcgga
     4621 tgtcgcaaaa gctctgcgga ccgcccggat gctggggctt ccggcaatag caacaactgc
     4681 ggagggcgac gcccaactgg acctgcaaat tgcgggggcc tgggggaatg gagccacggc
     4741 gcccagcacc gcatcgccgg ttacggggac ggtgaaactt cacaacgtgc gcgttgcaat
     4801 gcatggagtt ggggcgccgg ttgagatttc ctcggcagag atgcagcttt cgaacgagaa
     4861 ggtgcagatc gctaagttga gcgctgaagc ggccggagcg gcatggaacg gatcgattga
     4921 aatgccacga gggtgcggaa gtcccgacgc atgccaggtt catttcaacc tgaacgcgag
     4981 ccagattgtg ttaggggaac tgaaggagtg ggtgagccca cggccggcag agcaaccctg
     5041 gtatcgggta ttgacgaaag ccgcgccgtc ggcgccgtcg tttctatcaa gcctgcgagc
     5101 aaggggccgc gtgaccgcgg agcgattggt catccgaaat gttcccctga cgcatgtcgc
     5161 ggcaaatctt agtctggaga gcgggaacct gaagctatcc gatttgaccg cggaggttct
     5221 cggcggtaag catcagggag agtgggctgc tgacttcagc gcgacgccag cgccctgtca
     5281 tggcagcggg accctgaccg ggatagcgct gtcacaggct gcagacctga tgaaagactc
     5341 gtggatctcg gga
//
LOCUS       DASPUL010000606         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1889390, whole genome shotgun sequence.
ACCESSION   DASPUL010000606 DASPUL010000000
VERSION     DASPUL010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889390"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VEJ00_14005"
     CDS             complement(<1..288)
                     /locus_tag="VEJ00_14005"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXX02324.1"
                     /translation="MSDRTHRFDVLILGGGAAGLMCAIEAGKRGRRVAVLEHADRLGK
                     KILISGGGRCNFTNLHCQPENFLSSNPHFAKSALARYTPADFIALVEKHRIP"
     gene            complement(290..1015)
                     /locus_tag="VEJ00_14010"
     CDS             complement(290..1015)
                     /locus_tag="VEJ00_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02325.1"
                     /translation="MENYFNYFTEIEDRFLQRRRGGLLLSTLDWALIETWKDAGIPLE
                     AVLRGIDEAFDRYEERPSKTKRINSLAYCSQLVLSAAEDMKEAVVGAGDASQAAGKTR
                     ASQGFEPGAVAAYLRKNAAQLESAQLPQGTGVSVRTVALEAAKSLRQLAHEAEKSPPR
                     LQELERRLTVLEDKLFAALLAATADDEIVAVRAEADRDLAPYRRKMSAAQIDQLQKQY
                     VHKRLLEKYRLPRLSLFYMADFT"
     gene            complement(1022..1951)
                     /locus_tag="VEJ00_14015"
     CDS             complement(1022..1951)
                     /locus_tag="VEJ00_14015"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HXX02326.1"
                     /translation="MLLVLDVGNTNTVLGVFARVAKVHAGGDTDEPPRYERLLANWRV
                     ATSRTSTVDEYGVLFRNLFSMAGLEVKGIHGIVISSVVPPLDPVLRQVCERYFNLRPL
                     FIEPGVKTGMPVHYDNPAEVGADRIVNAVAAFDKYGGPCVIVDFGTATTFDCVSAKGE
                     YLGGVICPGIGISADALFERTARLPRVEIRKPARVIGSNTVGSLQSGLYYGYLGLVDG
                     IIELLLAELGPDTRVIATGGLGPMIGTGSKYIKHVDDFLTLDGLRIIWERNAAAKSTP
                     RADSKTASKQSKPEQKSRNGGGQAAVPTSRSAR"
     gene            complement(2004..2966)
                     /locus_tag="VEJ00_14020"
     CDS             complement(2004..2966)
                     /locus_tag="VEJ00_14020"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HXX02327.1"
                     /translation="MGRIVRLLMEHATVVVSGTKIAEEISSSRSEVWRLIQQLRSMGV
                     DVAGHPATGYQLRAVPDLLLPEILRPLVRGTLFSGHIHHFYKIGSTNTTAMAAAADGA
                     PEGTVYLAEEQTAGRGRGANSWQSPRSTGLYCSAVLRPAQPPSDVLVLSLAAGLAVQA
                     AIQQVDSRVQPDLKWPNDVLIEGKKVCGILTEMNAEATRVRYVVVGIGINVNQQGLPK
                     EFSATSLRLVTGSEWSRVELTAALLKSLDREYRQLGEDPAARESILRRFAANSSWVDG
                     KKVRIEENGISFEGTTEGLDPRGFLQLRTAYGTRTVLSGTVREA"
BASE COUNT          665 a         1073 c         1010 g          646 t
ORIGIN      
        1 aggaatccgg tgcttctcaa ccagcgcgat gaaatctgcg ggcgtgtagc gggccagcgc
       61 agacttggca aagtgcgggt tggacgacag gaagttttcg ggctggcaat gcaggtttgt
      121 gaaattgcag cggccgccgc ccgagatcaa aatctttttc cccagacggt cggcgtgttc
      181 gagaaccgca acgcgccgcc cgcgctttcc tgcttcgatg gcgcacatca acccggcagc
      241 tccgccgccg agaatcagca catcgaagcg atgggtccgg tcgctcattt cacgtaaagt
      301 cagccatgta aaacagactg agccgcggca ggcgatactt ctccagcaga cgcttgtgga
      361 cgtactgctt ctgcaattgg tcgatctggg ctgctgacat tttccggcgg tagggcgcaa
      421 gatcgcgatc tgcttccgca cggaccgcaa cgatctcgtc gtcggcagtc gcagccagta
      481 gcgcggcgaa gagtttgtct tcgagcaccg tcagacgccg ctccagctcc tgcaggcgcg
      541 gcgggctctt ctccgcctca tgcgcgagct ggcgaagact cttggcggct tcgagagcta
      601 cggttcgcac cgagacacca gtgccctggg gcaactgcgc gctttcgagc tgtgcggcgt
      661 tcttccgcag gtacgctgcg acggcgcccg gctcaaaacc ctgcgaggcc cgcgtcttcc
      721 cggccgcctg ggacgcatcg ccggctccga ccaccgcttc tttcatgtct tccgccgcag
      781 aaagcaccag ttgcgagcag tacgccaggc tgttgatcct ctttgttttc gatggacgct
      841 cctcgtagcg gtcgaacgcc tcgtcaatgc cgcgcagaac cgcctcgagc ggaatgcccg
      901 cgtccttcca ggtttcgatc agcgcccagt cgagcgttga gagcagcagc ccgccgcggc
      961 ggcgttgcag aaagcggtcc tcaatctcgg tgaagtagtt gaagtagttt tccacggaga
     1021 atcagcgcgc ggatcgcgaa gtgggaactg cagcctggcc gccgccgttc ctggatttct
     1081 gctcgggttt cgattgcttc gacgcagtct tggagtcggc tctaggcgtg cttttggcgg
     1141 cggcattccg ctcccagatg atccgcagcc catccagcgt gagaaagtca tcgacgtgct
     1201 tgatgtactt cgacccggtc ccgatcatcg gccccagccc gcccgtggca atcacgcgcg
     1261 tgtctgggcc gagttccgcc aggagaagct cgatgatgcc atccaccaga ccaagatatc
     1321 cgtaataaag ccccgactgc aggctgccga cggtgttcga cccgatcacg cgcgccggct
     1381 tgcgaatttc gacgcgcggt agccgcgccg tgcgctcgaa gagcgcgtca gcggaaatgc
     1441 cgatgccggg acatatcacg ccgccaagat attcgccttt cgccgatacg caatcaaaag
     1501 tcgtcgccgt accgaagtcg acaatcacgc acggcccccc gtacttgtca aacgcagcca
     1561 cggcgttgac gatgcgatcg gcgccgactt ctgccggatt gtcgtagtgc accggcatac
     1621 cggttttcac tccgggctca ataaacagcg gacgcaggtt gaaatagcgc tcgcacacct
     1681 gccgcagcac ggggtcgagc ggcggaacca ccgacgagat cacgatgccg tggatgcctt
     1741 tgacttcgag accggccatc gaaaacaggt tgcgaaacag gacgccgtac tcgtcgacgg
     1801 tgctggtgcg cgacgtggcg acgcgccaat tcgccaaaag ccgctcgtag cgcggaggct
     1861 cgtccgtatc gcccccggca tgcaccttgg ccacgcgggc gaagaccccc agcaccgtgt
     1921 tcgtgttgcc gacatccagg acaagcaaca taactaatct tcgactttct gggcttgcga
     1981 ggatctaggc caagctttcg ctttcaggct tcccgcaccg tcccgctcag caccgttctt
     2041 gttccgtagg cggtgcgcag ctgcagaaag ccccgggggt ccaggccttc ggtcgttcct
     2101 tcgaaagaga ttccattctc ctcaatgcga acctttttcc catcgaccca ggacgagttc
     2161 gcggcgaagc ggcgcagaat cgattcgcgc gccgccggat cctcccccag ctggcgatac
     2221 tcgcggtcga gcgattttag caaagcggcg gtcagttcca cgcgcgacca ctcgctgcca
     2281 gtcacgagac gcagcgaagt cgcagagaat tccttgggca aaccctgctg gttcacattg
     2341 attccaatac ccactaccac atagcgaacg cgcgtggctt cggcgttcat ctcagtgagg
     2401 atgccgcaaa ctttcttccc ttcaatcaac acgtcgttgg gccatttgag gtcgggttgt
     2461 acgcgggagt cgacttgctg aatggcagcc tggacggcca atccggcggc cagcgacagc
     2521 acgagcacat ccgacggagg ctgcgccggc cgcagcacgg ccgagcaata cagccccgtc
     2581 gaccgcggcg actgccagga gttggctccg cggccgcgtc cggcagtttg ttcttccgcc
     2641 agataaacgg tgccctccgg tgccccgtcg gccgccgccg ccatcgccgt cgtattggtc
     2701 gagccaatct tgtagaagtg atgaatatgc ccggagaaga gcgtgccgcg caccagtggc
     2761 cgcaaaatct cgggcaacag cagatcgggc accgcgcgca gttgatatcc ggtagcagga
     2821 tgccccgcca catcgacgcc catgctgcgc agttgctgga tcaggcgcca cacctccgag
     2881 cggctggagg aaatctcctc cgcgatcttg gtgccgctaa ccacgacggt cgcgtgctcc
     2941 atcagcaagc gcacgatgcg gcccaacctg acgtccgtgg gctgcgggtg gggtaacgag
     3001 gctccctgcg cgggcttccg cgcccgaatt ttgcccgtct tgctgatgcc gagattatgc
     3061 gcgaaccgct cagaaaggac aaacggtcgg gcacaaagct atatcgggga caataacggc
     3121 ggaaatcagc gcgagaggcc cgaacctatc tcgaatgcag caatagaaac acgcctgcta
     3181 acatggtcgt ggtttcaaga ggctggccag ccgaaatctc attgtcatga agaaaagcgt
     3241 gtggagagcc ataatcgaaa tcacgttcat catcttcctc ttttactcca acctgctcat
     3301 gggggaattt gaacgctccg gcatggggca aaagaggggg ctgtcatggg cgatcgcgga
     3361 tgttttcaca ggggcaaact tgaagattgg cgtg
//
LOCUS       DASPUL010000607         5917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1889913, whole genome shotgun sequence.
ACCESSION   DASPUL010000607 DASPUL010000000
VERSION     DASPUL010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889913"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..281)
                     /locus_tag="VEJ00_14025"
     CDS             complement(18..281)
                     /locus_tag="VEJ00_14025"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HXX02328.1"
                     /translation="MDQEARRKKQQEMMAELLERKIRLHAQQLYEQRGKADGMALQDW
                     VQAESEVLGTSIMAPFYRKLRLQENPENAPSSLPESSGCESSQ"
     gene            complement(432..1781)
                     /locus_tag="VEJ00_14030"
     CDS             complement(432..1781)
                     /locus_tag="VEJ00_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02329.1"
                     /translation="MNKPKILVMAALALCCGALYARQGSGPLTPFDKGTLSNSSHLTE
                     QGRQVFRFDTFGDQAFWGDTLHLHEAIEGSKLGGVGPGVSPKTALAVGLKVDLDALPA
                     PLVTELQQGQVNLDDPAVTLALLKLNSVVGVTGFFRPDGTLKSMGIQCALCHSTVDNS
                     LAPGIGHRLDGWANRDLNVGAIVALAPNLQPFASLLGVDQATVRKVLENWGPGHFDAE
                     LALDGKPFRPDGKTAAVLIPPAFGLAGVNLHTWTGWGSVTYWNAFVANLEMHGKGNFF
                     DPRLDNATQFPIAAKAGFGHVTNSTDLITPNLAALHFYQLALPAPAPPPGSFNEDAAK
                     RGEAIFNGKGKCSTCHVAPLYTEPGWNMHTPGEVCVDSFQADRSPDKRYRTSPLGGVW
                     THTKGGFYHDGRFPTLLDVVNHYDSCMSLALTDQDKSDLVQFLKSLPRPTSELSSIE"
     gene            1982..2701
                     /locus_tag="VEJ00_14035"
     CDS             1982..2701
                     /locus_tag="VEJ00_14035"
                     /inference="COORDINATES: protein motif:HMM:NF008888.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXX02330.1"
                     /translation="MASAALLEEAKRDGWSDEQIVTRVLAGETALYELLMRRYNQRLY
                     RVARAILRDDAEAEDVMQDAYVRAYQHLASFEGRAKFVTWLTRIAVHEALARSRKRSR
                     FQSLDVAEDSNGDVMKTATSSDRTPEQVTYDREMGRVLEKAILALSKEYRLVFMLRDV
                     EGMSTEETAECLNLTQENVKVRLHRAHAALRKQLHAAVGANAARCFQFPALRCDRVVE
                     GVFRTLGWSEKPLGSGDYEIP"
     gene            complement(2707..3273)
                     /locus_tag="VEJ00_14040"
     CDS             complement(2707..3273)
                     /locus_tag="VEJ00_14040"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HXX02331.1"
                     /translation="MTELGKDMVPKRIFFTKGVGKHRERLTSFELALRDAGIAAQNLV
                     RVSSIFPPNCKLLTRKEGVKYLHPGEVVFAVVAENSSREPHRLLASSIGVAIPADRNT
                     YGYLSEHHSFGETEDAAGEYAEELAAEMLATTLNVEFDPDRSWDEKKQIYRLSNKIVR
                     TANVTQSAVGDKRGLWTTVIASAVLIFD"
     gene            complement(3422..3772)
                     /locus_tag="VEJ00_14045"
     CDS             complement(3422..3772)
                     /locus_tag="VEJ00_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02332.1"
                     /translation="MKSRLQKLTFAVMTLALTTSLAVAAFAAKGQTFTGTVSDAMCGA
                     KHAMAGDDAGCTRACVSKGSKYALVVGDKVYTLETSDKAALAALDKQAGAKATVTGTA
                     KGDMITVASVMAAK"
     gene            complement(4189..5460)
                     /locus_tag="VEJ00_14050"
     CDS             complement(4189..5460)
                     /locus_tag="VEJ00_14050"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HXX02333.1"
                     /translation="MSQLPASARQLIAIKVTGSKRFSEADIAAATGLQLGTAITDDDF
                     KKAARRLGDEGAFADITYSYTYSLAGTKLEFKVTDSDKFVPAHFEDFVWFSDAEMNRR
                     IREHVPLFNGELPASGRMPDEVSDVLQAMLVEGAIPGHVNYVRRGKQDGPLESIDYSV
                     SDVIIRVRNIDFVGAGAAELPALENAAQKFSGRSYSRTQLDAFVQRQVLPVYHERGYL
                     KATCGEPQSKVVQQPGDRAAAEPEDGPRNQTIVDVTFALTPGLQYKLKGVEWSGNHEF
                     PTDTLEKMLRAPLDQPADTVRLAENLKEVQQLYGSRGFVVAAVQANAQFDDDAANVVL
                     HLEVKEGSVYRMGDLEFRGLDNSLTAKLRNAWKLRPGDVYDATYLDQYLPEAQKLLPS
                     RLDWEVTPHVTANVRDKTVDVDLIYSVKAPD"
     gene            complement(5550..>5917)
                     /locus_tag="VEJ00_14055"
     CDS             complement(5550..>5917)
                     /locus_tag="VEJ00_14055"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HXX02334.1"
                     /translation="VADVAKFPLVVPRAGHTRDAIEQLFHERRLKPNFTMELDSSELL
                     KRFVAADVGVGFIARSNVDEDVRARVLAAVEIADAQIRRDLALVFRKDKALSRAALAF
                     IDIAVKHKSTQELVQAPAR"
BASE COUNT         1174 a         1667 c         1843 g         1233 t
ORIGIN      
        1 gtttgggtaa gggagagtta ctggctggac tcgcagccgg atgactcggg cagggaagaa
       61 ggcgcattct cagggttctc ctggaggcgc agcttgcggt agaacggcgc cattatgctt
      121 gtgcccagca cctcggactc ggcctgcacc cagtcctgca gtgccatgcc gtcagccttg
      181 ccgcgctgtt catacagctg ctgggcatga aggcggattt tgcgctccaa aagctcggcc
      241 atcatttcct gctgtttctt cctgcgtgct tcttgatcca ctgtctactc caaaagggat
      301 acgtgcttga tcacagttcc tatgatagcg gatcgttgtt aacgaaagat tacacaggga
      361 agctgcctgt ggttactaaa gttagaccct acttgtcaaa gtgggaggtg catcatcagg
      421 cgttaacctg ttcattcgat gctgcttagt tcacttgtcg ggcgtggcag cgatttcagg
      481 aactgtacca aatcagactt gtcctgatcg gtcaaagcca ggctcataca gctgtcatag
      541 tgattcacaa cgtcgagcaa agtagggaat cgtccgtcgt ggtaaaagcc gccttttgta
      601 tgcgtccata cgccgcctaa aggcgacgtg cggtagcgct tgtcgggcga acggtcggcc
      661 tggaagctgt cgacgcatac ttcacccggt gtatgcatgt tccatcccgg ttcggtgtag
      721 agaggcgcta cgtggcaggt cgagcatttg cccttgccgt tgaaaatcgc ctcgcctcgt
      781 ttggcggcgt cctcgttgaa actgccggga ggaggcgcgg gcgcggggag ggccagctga
      841 taaaaatgca gcgcggccaa atttggcgtg atgagatccg tcgagttggt cacgtggccg
      901 aagccggcct tggccgcgat cggaaactgc gttgcgttat cgagccgtgg atcgaaaaag
      961 ttgcctttgc cgtgcatttc aaggttcgcg acgaatgcgt tccagtaggt gaccgaaccc
     1021 catcctgtcc aggtgtgaag attcacgcca gccaggccga atgcaggagg aatcagcacc
     1081 gcggcggttt ttccatctgg ccgaaagggc tttccgtcaa gggcaagttc ggcatcgaag
     1141 tgccccgggc cccagttctc cagcaccttc cgcaccgttg cctgatcgac tcccagcaga
     1201 ctggcgaagg gctgtaaatt gggagcgagc gcgacaatcg cgcctacgtt caaatcgcgg
     1261 ttcgcccagc cgtcgaggcg atgtccgatg ccgggagcaa gagagttgtc gactgtggag
     1321 tggcacaacg cacactggat tcccatcgac ttcaacgttc catcgggcct gaaaaaacca
     1381 gtgacgccga ccacagaatt gagcttgagc agcgcaagcg tgactgcagg atcatccagg
     1441 ttcacctggc cctgctgcaa ctccgtgacg agcggggcgg ggagcgcgtc gaggtcgact
     1501 ttcagcccga ccgccaatgc ggtcttcggg ctgactcccg gacctacgcc cccgagcttg
     1561 ctgccttcaa tggcctcgtg gaggtgcagc gtgtcgcccc agaaagcctg atcgccgaac
     1621 gtgtcaaagc ggaagacttg ccggccttgc tccgttaggt gcgacgaatt tgagagcgtg
     1681 cctttgtcaa atggcgtcag tggaccgctg ccctggcgcg cataaagagc gccgcagcaa
     1741 agtgccagcg ccgccatcac gaggatcttc ggcttgttca tattgcctcc gcaagggaaa
     1801 ggagtgaatc gaagccgtgc ggttacagat cgtctgcgga aagctgtcga aagcccgtcg
     1861 cagctgcatg gacaaagatt tgtgcgcctg cgcgcaatcc cgggaacaca gaatgaaaat
     1921 tgtttgtaac cgcgggcggg ctcgcgactc attctgttct agggctgttc tgaggttgcc
     1981 tatggctagt gctgcattgc tcgaagaagc gaagcgggac ggttggagcg acgaacaaat
     2041 tgtcacccgt gtgctcgccg gggagacggc gctctacgaa ctgctgatgc gccgctacaa
     2101 ccagcggctc tatcgcgtgg ctcgagcgat cctgcgcgat gatgcggaag ccgaagatgt
     2161 catgcaggac gcctacgtcc gcgcctatca gcatttggcc agcttcgagg gccgcgccaa
     2221 gttcgtgacg tggctgacgc gcattgccgt ccacgaagcc ctggcgcgaa gtcggaaacg
     2281 ttcgcgcttt caatccctcg atgtggcgga agattcgaac ggagacgtta tgaagacagc
     2341 aacttccagc gaccgtaccc ccgaacaggt gacttatgac cgggagatgg gcagggtact
     2401 ggagaaggcg attctggccc tgtcgaagga atatcgcttg gttttcatgc tgcgcgacgt
     2461 ggaaggaatg agcacggaag aaactgccga atgcctgaac ctgacccagg agaacgtcaa
     2521 ggtgagactg caccgggctc atgccgcgtt aagaaaacag ctgcatgccg cggtaggagc
     2581 caacgctgcc cgctgcttcc agtttcctgc gctgcggtgt gaccgggtgg tggagggcgt
     2641 atttcggact ttgggctgga gcgaaaagcc acttggaagc ggcgattacg aaatcccgta
     2701 gtccgcttag tcgaagatta ataccgccga ggcgatcacg gttgtccaca aaccgcgctt
     2761 gtcgccgacg gccgactgcg tgacgttcgc tgtgcgcacg atcttgttcg acagccggta
     2821 gatctgcttc ttctcgtccc aggagcggtc gggatcgaat tccacattca gtgtagtggc
     2881 cagcatctcg gcggcgagtt cttctgcgta ctccccggcg gcgtcttcgg tctcgccgaa
     2941 cgaatggtgc tcgctaagat atccgtaggt attgcgatca gccggaatgg ctacgccgat
     3001 cgacgacgcc agcaggcggt gcggttcgcg gctggagttc tcggcaacca cggcaaagac
     3061 gacctcgccc ggatgcaggt acttcacgcc ctctttgcgg gtgagcagtt tgcaattggg
     3121 cggaaagatg gaggagacgc gcaccaggtt ctgggcggcg atgccggcgt cgcgcagcgc
     3181 caattcgaaa gacgtcaacc gttcgcggtg tttccccact cccttggtaa agaaaatccg
     3241 cttcggaacc atgtctttgc ccaattccgt catcctccgg ccgagaaatt ttgtgcttgg
     3301 gaatgcacaa tctaacacat cgctttgtaa aggaaatatg aatttgcgag gtgggacaag
     3361 aaaatacagg aggcgcggga gcgcctcccg gcttgcgaaa ggagtgggaa gggctggggc
     3421 tttacttggc tgccatgact gaggcgaccg taatcatgtc ccccttggcg gtcccggtca
     3481 cggttgcctt ggcgccagcc tgcttgtcga gcgcggccag agcggctttg tcgctggtct
     3541 ccagggtata gactttgtcg ccgacgacca gggcgtactt cgagcctttg ctgacgcagg
     3601 cccgcgtgca accggcatca tcgccggcca tggcatgctt ggctccgcac atcgcgtcac
     3661 tgacggtgcc agtgaaggtt tggcccttgg cggcgaatgc ggcgacggcc aggctggtgg
     3721 taagggccaa ggtcattacg gcgaatgtga gtttctgtaa gcgagacttc atgcgaattt
     3781 tctccttgat agttttcgta cgctcttacc ccgcagggac gggtaatttc cccgaatgaa
     3841 agatgtggtc agcggcgaaa ggttacagtg tccgtccgtg gggccgctgt agacgctgcc
     3901 gacccgctta agccatccta caagaaaacg cggggacgca ctggcgtgca tccccgctga
     3961 tgcccggatc acaatcgggg gagggcccgg gcgaaaccgt ctcgtgacgc gttaggtgcc
     4021 gcctcagact caactcgagg ctagcccaca gtctgcccct cgtctgaggc aaggacctct
     4081 aagaagtgcc tagcgtacac ccacaagtgc ctgtttgcaa aacttgtttt gcggttgttc
     4141 cggaaagcgg actgagaggg tcagttccta ccgcctaatc cttcggtctt aatcgggggc
     4201 tttaaccgag tagatcaggt caacatccac cgttttgtct cggacattgg cggttacatg
     4261 cggcgtgacc tcccagtcca gccgggaggg aagcagcttt tgcgcctccg gcaggtactg
     4321 atcgaggtaa gtagcgtcgt agacgtctcc cgggcggagt ttccaggcat tgcggagttt
     4381 ggccgtcagg ctgttgtcga gcccgcggaa ttcgagatcg cccatgcgat aaaccgagcc
     4441 ttccttcacc tcgagatgga gaaccacgtt ggcggcgtca tcgtcgaact gggcgttcgc
     4501 ctgaaccgcg gccacgacaa atccgcggga gccataaagt tgctgcactt ccttcagatt
     4561 ttcggcgagc cgcacggtat cggcgggctg atcgagaggg gcacgcagca ttttctcgag
     4621 cgtgtcggtg gggaactcgt ggttgccgga ccactcaact cccttcagct tgtactgcag
     4681 tccgggagtg agagcaaacg taacgtccac gatggtctga ttgcgtggcc cgtcctccgg
     4741 ctctgctgca gcccggtcgc ccggttgttg cacgactttc gactgaggct ctccgcacgt
     4801 ggctttgagg taaccccgct catggtagac gggtagaacc tgacgctgga cgaacgcgtc
     4861 cagctgcgta cgcgagtagg agcggccgga gaatttttgg gcggcgttct ccagtgcggg
     4921 caattcggct gcgcctgccc cgacaaagtc gatgttgcgg acccggatga taacgtcaga
     4981 cacgctgtag tcgatcgatt ccagcggccc gtcttgcttg cccctgcgca catagttcac
     5041 gtggccgggg atggcgcctt cgaccagcat cgcctgcagc acgtcggaaa cttcgtcggg
     5101 catgcgcccg gatgccggga gttccccatt gaagagcgga acatgttccc gtatccggcg
     5161 gttcatttcc gcgtccgaga accagacaaa gtcctcgaag tgcgcgggga cgaacttgtc
     5221 ggagtccgtc accttgaact caagtttcgt gccggccagc gaataagtat aagagtaggt
     5281 aatgtcggca aaagcgcctt catcgcccag gcggcgggcc gctttcttga aatcgtcgtc
     5341 ggtgatggcg gtgccgagtt gcaggccggt ggcggcagcg atgtccgcct cagaaaagcg
     5401 cttgctcccc gtgactttta tggcaatcaa ctgacgggcg gaggcaggaa gctgagacaa
     5461 gggcttctgc gaggacgagg cttgtccccg gcccgacagc aacagtgccg ggaggagcag
     5521 aggcagcaat agtctacgga ctgcaggggt catcgcgccg gcgcctgcac taattcttgc
     5581 gtggatttgt gcttgacggc aatgtcgatg aaggccaggg cggcgcggct cagcgccttg
     5641 tctttgcgga agaccagcgc cagatcgcgg cgaatctggg cgtcggcaat ctccaccgcc
     5701 gccagcactc tggcgcgaac atcttcatcc acgttggagc gtgcgatgaa gcccacaccg
     5761 acgtcggcgg ctacaaagcg cttcagcaat tcgctggaat cgagttccat ggtgaagttc
     5821 ggcttcaaac ggcgttcgtg aaacagttgt tcgatagcgt cacgcgtgtg tccggctctg
     5881 ggtacgacca gggggaactt cgccacgtcg gcgaccg
//
LOCUS       DASPUL010000608         4123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_796287, whole genome shotgun sequence.
ACCESSION   DASPUL010000608 DASPUL010000000
VERSION     DASPUL010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796287"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="VEJ00_14060"
     CDS             complement(<1..1918)
                     /locus_tag="VEJ00_14060"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXX02335.1"
                     /translation="MRRLFLLLLAFSFFAVSVAGQAKHPFTFEDMMKLKRVADPQVSP
                     DGKWVIFSVVDVDLEANTRTPHVWIVPLNDDPHVSQKPGDMGHPGGSEEKSGVPHVSP
                     NLRDVRGTERELISDQDADRPRWAPDGKRFAFLSTKEGGSQVWIAEFDVANGTVTAVN
                     KLTSIATEASGELWSPDGKHILFVSDVYPECDRKPGGEIDAQGEQSCNEQKLKEANES
                     KVRAQIFTHLLYRHWNAYKQGRRSHIFVTKSDLPITVSNNQGTLKLLHTPYTLTPGDY
                     DAPVFSLGGQDDYAFSPDGKQICFTSNHDKNPAASTNNDLWLAWVNGGGEPENITADN
                     PASDSTPLYSPDGRYIAYRAQQRPGYEADEFRLMLYDRTTGDKRKLYTAAFWVTEFAW
                     SPDSSKIFYPGSDEDARNSGQTVIWSSTVGPKMVRTEALPKQVPENLRKKMQPITKLF
                     FSERITRGYDGDLAFTPDGKTLVFTRMSLQHPVEIRKLVFPEKIDPYDLPEGEQLTHV
                     NDAILSQVDMQPLEPFSFTGAHDDKVQGFLVKPPNFDASKKYPVKFLIHGGPEGAWGD
                     DWSYRWNPELFASPTSATPSGYVVVMINFHGSTGYGQKFVDAINGDWGGAPFEDLMKG
                     LDYAEQNYPFIDKDR"
     gene            2293..3144
                     /locus_tag="VEJ00_14065"
     CDS             2293..3144
                     /locus_tag="VEJ00_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02336.1"
                     /translation="MKLGFRPALLFLTLVASAVVALPALAQEGPLQNTPPQGTTPDEI
                     IKRFAAKETEFAKAREQYTYRQDVKVETLDGDTIDGQYHEVFDVIFDDKGKRLENVVF
                     APQSNLTRIYMSQEDMEDIRHKLPFVLTEQELPEYNILYVGQQQEDELHCYVFDIAPK
                     EIVGKKRYFQGRVWVDDHDFQIVKTYGKTVPDIRKHHGDEENLFPKFTTWRQQIDGQY
                     WFPVYTKADDELHFKTEDVHIVEIVKYEDYKRFGTNVKILYEGKELPKDQPKPGQKPP
                     DQGSPQK"
     gene            complement(3152..>4123)
                     /locus_tag="VEJ00_14070"
     CDS             complement(3152..>4123)
                     /locus_tag="VEJ00_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02337.1"
                     /translation="EYYKSDYDRLAPQLHADERTALANLKRRIKDENGGIISAQLALY
                     FSATDAESIDELIRAVKDSSAMQRNLKATPYYDEKQWKVYEASRPDLERAFNALKRIH
                     FEDDWEKHAEPAVEQAIATIAKDLSSYNVIPEVESVLGAPRPTNKITIYMLYYSQPHG
                     IKITGTRFLTHYSYPFRIVLRNAIHEMMHPPYDLAHDPDLRASLQSLHADSYLMDKVE
                     HHNRSYGYNTLEGLEEEDCVQSLEQVIAERFGMGGDPRRYWREQDDGIHVLAVALYSL
                     MKQKGFAGGREAFPAFLKRTIQSGALSNGGIEELNRAFFSPPVAKPD"
BASE COUNT          853 a         1158 c         1215 g          897 t
ORIGIN      
        1 cgcggtcctt atcgatgaac ggataattct gctcggcgta gtcgaggccc ttcatcaggt
       61 cttcaaacgg agcgccgccc cagtcgccgt tgatggcgtc gacgaacttc tgcccgtagc
      121 cggtcgagcc gtggaagtta atcatgacga cgacgtatcc gctgggcgtg gcggaagttg
      181 gtgacgcgaa cagctcggga ttccagcgat acgaccagtc gtctccccac gcgccttcag
      241 gcccgccgtg gatgaggaat ttcacgggat atttcttgga ggcgtcgaag ttcggcggct
      301 taacgagaaa accctggacc ttgtcgtcgt gcgcgccggt gaacgagaag ggttcgagcg
      361 gctgcatatc gacctgggag aggattgcat cattgacgtg ggtgagttgt tcgccttcgg
      421 gcaaatcgta cggatcaatt ttttcgggga aaacgagctt ccgaatttcc acgggatgct
      481 gcaatgacat tcgcgtgaag accaaagtct tgccgtcggg cgtaaaggca aggtctccgt
      541 catagccgcg cgtgatccgc tcgctaaaaa acagtttggt gatcggctgc attttctttc
      601 gcaggttctc cggaacctgt ttcggcagcg cttcggttct caccattttt ggccctaccg
      661 tcgatgacca gatcacggtc tgtccgctgt tgcgtgcatc ttcatccgat ccgggataaa
      721 agatcttcga cgagtcgggc gaccaagcaa attcggtcac ccagaaggcc gccgtataga
      781 gtttcctctt gtccccggtt gtcctgtcgt agagcattag ccgaaactcg tcggcctcgt
      841 atcccgggcg ctgctgggcg cggtaggcga tgtagcgtcc gtcgggcgaa tagagtggcg
      901 tcgaatcgct ggccgggttg tcggcggtga tgttttctgg ctctcctcct ccgttgaccc
      961 aggcgagcca gagatcgttg ttggtggagg ccgcgggatt cttgtcgtga ttcgaggtga
     1021 agcagatctg ctttccatcc ggagagaatg catagtcgtc ctgtccgcca agcgagaaca
     1081 cgggcgcgtc gtaatctcca ggagtcaacg tgtatggagt gtggagaagt ttcaacgtgc
     1141 cctgattgtt gctgaccgta atcggaagat cggacttggt gacgaaaatg tggctgcgcc
     1201 tcccttgctt gtaggcattc cagtggcgat agagcaggtg cgtgaagatc tgcgcccgaa
     1261 ctttcgactc gtttgcttcc ttgagctttt gttcgttaca ggattgttct ccctgggcat
     1321 cgatctctcc gcccggcttc cggtcacatt ccgggtacac gtctgagacg aacaaaatgt
     1381 gcttgccgtc cggagaccac aactcgccac tggcttcggt tgcaatcgaa gtcaatttat
     1441 tcactgccgt cacggtcccg ttcgcgacat caaactccgc gatccacacc tgcgaaccgc
     1501 cctctttcgt cgacagaaac gcgaagcgct tcccatccgg cgcccagcgc gggcggtcgg
     1561 cgtcctgatc cgagatgagt tcgcgttccg tgcctctcac gtctcgcaaa ttcggcgaga
     1621 cgtggggcac cccacttttt tcttcactcc cgccggggtg ccccatgtct cccggttttt
     1681 gggagacgtg gggatcatcg ttgaggggca cgatccagac atgcggcgtc ctggtattgg
     1741 cttcgagatc gacatcgacc acgctgaaga tcacccactt gccgtcgggc gagacctgcg
     1801 gatcggcgac gcgcttcagc ttcatcatgt cttcgaaggt gaagggatgc ttggcctgac
     1861 ctgccacaga cactgcgaaa aaagagaatg cgagaagcaa aaggaagaga cggcgcatga
     1921 aggcacctca cacagatcaa tgcggcaaac ggatgagtgt agcaggggct cgcggcgagt
     1981 tctaggtgaa gcaccgatgc gcgcccccgg cacaatgcat aggtccttcg cttcgctcac
     2041 gatcacaaaa gatgacaaac cagaatgact cggcgaatgt ctcctcgggc gcggcaaccc
     2101 cgcggcatcc cgcgcatcca acaagaaggc agaactgcag cgccctcccc tccggcctcg
     2161 ctgagcggag aggggacgga gggagggttg ccgcggtcgt tttttgtttg cctcgacacc
     2221 tcgtgcaggg ccactggcag ttacgggtac catctccacg acattccgaa gccgattcga
     2281 taaactagac ctatgaaact ggggttccgt ccagctctgc ttttcctcac gcttgtagcc
     2341 tccgccgtgg ttgccctgcc agccctcgcg caggaaggtc cgctccaaaa cacgccgcca
     2401 cagggcacca cgcccgacga gatcatcaag cgctttgccg ctaaggagac cgagttcgcc
     2461 aaggcgcgtg agcagtacac ctaccggcag gacgttaagg tggagactct cgacggggat
     2521 acgatcgacg gccagtatca cgaagtcttt gacgtgattt tcgacgacaa gggcaagcgc
     2581 ctggaaaacg ttgtgttcgc gccgcagtcg aacctgacca ggatctacat gtcgcaggag
     2641 gacatggaag atatccggca caagctgccc tttgtgctga ccgaacagga attgcccgaa
     2701 tacaacattc tttacgtcgg ccagcagcag gaagacgaac tccactgtta cgtcttcgat
     2761 atcgcaccta aggaaattgt cggcaagaaa cgttatttcc aggggcgcgt gtgggtcgac
     2821 gaccacgatt tccagatcgt gaagacttac ggcaagacgg tgcccgacat ccgcaagcac
     2881 cacggcgacg aggagaatct gtttcccaag tttacgacgt ggcggcagca gatcgacggc
     2941 cagtactggt tcccggttta taccaaggcc gacgacgagc tgcacttcaa gaccgaagac
     3001 gtgcacatcg tagagatcgt gaagtacgag gactacaagc gtttcggcac caacgtgaag
     3061 attctctacg aaggcaagga attgcccaag gatcaaccca agccaggcca gaagccgccc
     3121 gatcagggct caccgcagaa gtgaggcttt atcagtccgg cttcgccacg ggcggcgaaa
     3181 agaacgcgcg gttgagttcc tcaatgcctc cattcgacag agcgccggat tgaatcgtgc
     3241 gcttcaggaa tgcgggaaaa gcttcgcgac cgccggcgaa tcccttctgc ttcatcaggc
     3301 tgtagagcgc caccgcgagc acgtgaatgc catcgtcttg ctcacgccaa tagcgacgcg
     3361 gatcaccgcc catcccgaag cgttccgcga tgacctgctc cagcgactgt acgcaatcct
     3421 cttcttccaa accctccaaa gtgttgtatc cgtacgaccg gttgtgatgc tcgaccttgt
     3481 ccatcaggta agagtcggcg tgcagcgatt gcagagatgc gcgcagatcg ggatcgtgtg
     3541 ccaggtcgta cggagggtgc atcatttcgt gaatcgcatt gcgtagcacg atgcggaaag
     3601 gatacgagta gtgcgtcagg aagcgcgtgc ccgtgatctt gatgccatgc ggctgcgagt
     3661 agtacagcat gtagatcgtg attttgttcg tgggacgcgg cgcgccgaga acggactcca
     3721 cttcaggaat gacgttgtag ctggacaggt ctttggcgat cgtggctatg gcttgctcga
     3781 cagccggctc ggcgtgcttc tcccaatcgt cttcgaaatg aatgcgcttg agcgcgttga
     3841 aggctctctc cagatcgggt cggctggctt cgtagacttt ccattgcttc tcatcgtagt
     3901 aaggagtggc tttgagattg cgctgcatgg cggagctgtc tttcaccgcg cggatcagtt
     3961 catcgattga ttctgcgtcg gtggcagaga agtagagtgc gagctgagcc gatatgatcc
     4021 cgccattttc gtctttgatg cggcgcttca ggttggcaag cgctgtgcgc tcgtcggcgt
     4081 gcaactgcgg agccaggcgg tcatagtcag atttgtagta ttc
//
LOCUS       DASPUL010000609         5844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_951671, whole genome shotgun sequence.
ACCESSION   DASPUL010000609 DASPUL010000000
VERSION     DASPUL010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951671"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VEJ00_14075"
     CDS             complement(<1..806)
                     /locus_tag="VEJ00_14075"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HXX02338.1"
                     /translation="MPELPDITAYIAALEARIIGEPLEHIRLASPFLLRTVQPPLAAA
                     EGRTVRSLRRIGKRIAIGLEGDLWLVMHLMIAGRLHWRPPNVKLAGRQNLAAFDFPNG
                     SLVLTEAGSKRRAALHLISGEQNLASLDAGGIDVFAATLEDFRAALTAENHTLKRALT
                     DPRLLSGIGNAYSDEILHAAQLSPVTLTQKLSQEDWERLFAATGQTLRKWIDRLRADA
                     EQDFPEKVTAFRKDMAAHGRYGLPCPRCGDKILRIRYADKETNYCARCQTG"
     gene            947..1996
                     /gene="hemH"
                     /locus_tag="VEJ00_14080"
     CDS             947..1996
                     /gene="hemH"
                     /locus_tag="VEJ00_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HXX02339.1"
                     /translation="MTRTTRRVGIVLFQLGGPDTLEAIEPFLYNLFCDPDIIDFPFAR
                     IGRKPLAKLISTTRARKVQQHYATIGGGSPIRRHTEQQARALEAELRRQGIDAHCFVA
                     MRYWHPFTEEAIAQLRTAECEEVVLLPLYPQYSSTTTGSSLNEWQRLFHDDVPMHCVV
                     NFYRNTMYLDAVVEKVNQALSRFPAPQKPEIVFSAHGIPVSVVDCGDPYQRQIEETVQ
                     LLMQRAGWSNGHTLCYQSKVGASKWLQPSLHQTIRKLSADGVREVCVVPVSFVSDHVE
                     TLGEIDHEAREEAARLGIRQFEMSGGLNDSPRFVAALAQVVEEALAKRVPSSVPDQSA
                     GRQWATPEYAPAAGD"
     gene            2051..2680
                     /locus_tag="VEJ00_14085"
     CDS             2051..2680
                     /locus_tag="VEJ00_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXX02340.1"
                     /translation="MAETDKVNPSPEAAKPAATVDATKYVGTPAVGTSKAAAAAGVPT
                     TVPSTEIDRRRRRFVWTMVTGFLTAWFLAFFRFFLPRTLFEPNTVFKIGYPSEFALGV
                     DTKFQQKYRIWVDRTPDRLFVIYARCTHLGCTPDWKPSENKFKCPCHGSGYDSEGINF
                     EGPAPRPMDRAHIELAPDGQVLVDTSKLYSWPKGQPTHFNDPGAFLTGV"
     gene            2753..3691
                     /locus_tag="VEJ00_14090"
     CDS             2753..3691
                     /locus_tag="VEJ00_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HXX02341.1"
                     /translation="MADENNGKNGKVGLVERVTTGVKDDIQALKTDIPTKAKEQIEGL
                     KDPTKTQVYTSIFRHKHDDTPRNRALGVLSNVFLHLHPAKINRDAVRYSYTWGMGGIT
                     FYLFIVLTYTGVLLMFYYHPSKVQAFRDVLYLEHDVPFGKILRNMHRWAAHLMVIAVW
                     LHMFRVFLTGSYKKPREFNWNIGVLLLVFTLLLSFTGYLLPDDQLGFWAVTVGTNMAR
                     ATPLLGHEGPFGPQLGMTPYNDVRFGLLGGSIVDANALLRSYIWHCIGIPIVASVLMI
                     VHFWRVRKDGGISGPAQVVLAAEADKPRRGPKAAGE"
     gene            3831..4778
                     /locus_tag="VEJ00_14095"
     CDS             3831..4778
                     /locus_tag="VEJ00_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02342.1"
                     /translation="MDWRQLWEICSAPDNVPIVGLIPLLIFYIYLAWKQAHANDQLIA
                     QLEASPEMAKTHHRKTWPFKPGWQKEVHVWPFLLRIEFLAAIIVTIILMVWSITLNAP
                     LEEPANPNLTMNPAKAPWYFLGLQEMLVYFDPWIAGVVMPTMIIIGLMVIPYIDTNPL
                     GSGYYTWKQRRFSIATFLFGFIILWVAMIIIGTFIRGPGWQWFWPGQTWDHNRLIYEV
                     NRDLPDEVPRWLGGPITSNWAKGVFGAIVVGGYYLIGGGAVYSLFRRKMQKDFQRMSL
                     LQFSIMASLWLTMVALPIKMGLRLVWHIKYVWITPWFNV"
     gene            4958..>5844
                     /locus_tag="VEJ00_14100"
     CDS             4958..>5844
                     /locus_tag="VEJ00_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02343.1"
                     /translation="MPEKPTPEQDPITSKSYAAHYAIATVILVITLFWALWDEAWGQR
                     PWKEFQEQWKTRYTAFLNTAKSKSQTSEKDVEKNSDYATLYAAWKKASDDSAPQAKKV
                     RDELNDASARLLAVQSVFTDRRAYVNALTYELETSESQSSKASKQKDIDAYKAEQATV
                     EFPDGSKKKFNFQQLEETYNEIRDERTKLSLELGDVLKPATDAKNKLDEYVTDHMVDL
                     TPAQIDGLKKKAADWDPEIKQINVAEANIVDRCESCHMSSREPLKITAASMTLKGEKK
                     PDEYAQALVSHTEPELLQI"
BASE COUNT         1242 a         1667 c         1761 g         1174 t
ORIGIN      
        1 ccggtctggc atcgggcgca atagtttgtt tccttgtcgg catagcgaat gcgcagaatc
       61 ttgtcgccgc agcgcgggca cggcaagcca tagcggccat gcgcagccat gtccttgcga
      121 aacgcggtga ccttttccgg aaagtcctgc tctgcgtcgg cgcgaaggcg atcgatccac
      181 ttgcgaagcg tttgcccggt ggcggcgaat agccgctccc aatcctcctg actgagcttc
      241 tgggtaagcg tgaccggcga aagctgcgcg gcgtgcagga tctcgtcaga ataggcgttg
      301 ccgatgccgc tgagcaggcg cggatccgtc agcgcgcgtt tgagggtatg attctcggcc
      361 gtgagcgccg cacgaaagtc ctcgagagtt gccgcgaaca catcgattcc tccggcgtca
      421 agcgaagcca ggttctgctc gccgctgatc agatgaaggg ccgcacgccg ctttgagcca
      481 gcttcggtca gaaccagcga gccattggga aaatcgaacg ctgccagatt ctggcgcccc
      541 gccagcttga cgttcggcgg gcgccagtgc agccgccctg cgatcatcag atgcatcacc
      601 agccagagat ccccctccag accaatggca atgcgttttc cgatacggcg gagactgcgg
      661 accgtgcgac cttcggctgc tgccagcgga ggctgcacgg ttcgcagcag aaaggggctg
      721 gccaaccgga tatgctcaag cggttcaccg ataatgcgcg cttccagcgc cgcgatgtaa
      781 gccgtaatgt cgggtagttc gggcaccgga taccagcttg cacgccaaca gcaggatttt
      841 gcaatccgac gatggcgccg gagagtccca ctgagatttt gcacattccg ccattgactc
      901 tcgatgcgga tgagagtaaa ttcgcccgtt ctggggattc tggggaatga cgcgcacaac
      961 acggcgtgtg gggatagttc tatttcagct tggcggtcct gacacgctgg aagcgattga
     1021 accttttctt tataacctct tctgcgaccc cgacatcatt gacttccctt tcgcgcgcat
     1081 tggacgcaaa ccgttagcaa agctcatttc gaccacgcgc gcccgcaagg tgcagcagca
     1141 ctatgcgacc atcggcggag gctctcccat ccgccggcac accgagcagc aggcgcgtgc
     1201 gcttgaagcc gaactccgcc gtcaaggtat cgacgcccac tgtttcgtcg ccatgcgcta
     1261 ctggcatccg tttaccgagg aagcgatcgc acaactgcgg acggcggagt gcgaagaggt
     1321 cgtcttgctt ccgctctacc cgcagtactc ttcgacgacg acggggtcga gtctcaacga
     1381 gtggcagcgc ctgttccacg acgacgtgcc gatgcattgc gtggtgaatt tctatcgtaa
     1441 tacgatgtat ctggatgcgg tggtggagaa ggtcaatcag gccctgtcgc gcttcccagc
     1501 cccgcagaaa ccggagatcg tcttcagtgc gcacggcatt cccgtgtctg tcgtggactg
     1561 cggcgatccc taccagcgac agatcgagga gactgtgcag cttctgatgc agcgcgctgg
     1621 ctggagcaac gggcacacgc tttgctacca gagcaaagtg ggggcaagca aatggctgca
     1681 gccctccttg catcagacca tcaggaagct ttctgctgat ggggttcggg aagtctgcgt
     1741 cgtgccggtg tcgttcgtgt ccgatcacgt cgaaacgctc ggcgagatcg atcacgaagc
     1801 gcgcgaggag gcggcccggt tgggcatccg gcagtttgag atgagcgggg gcctgaacga
     1861 ctcgcccagg ttcgtcgcgg cgctggcgca agtcgtggag gaagcgctgg cgaagcgggt
     1921 accgtcgtcc gtgccggatc aaagcgctgg gaggcagtgg gcaacgccgg aatacgcacc
     1981 ggccgcgggc gactgatatt cggcccccgc gacaaccggt gagcgaaagc aaggaaagtg
     2041 gagggagttc atggcggaga ccgataaggt caatccgagt cccgaggcag cgaagcctgc
     2101 tgccaccgtg gatgccacaa aatatgtagg cacgcctgca gtggggactt cgaaagccgc
     2161 ggcggcagcg ggcgtgccca caactgttcc ctcgaccgag attgaccgcc ggcgccggcg
     2221 ttttgtttgg accatggtta cgggcttcct gaccgcatgg ttcctggcct tcttccgatt
     2281 ctttctcccg cgcactcttt tcgaacccaa caccgtcttc aagatcggat acccgtcgga
     2341 gtttgcactt ggcgtcgaca ccaagtttca gcagaaatac cgcatctggg tcgaccgcac
     2401 acctgaccgg ctgttcgtga tctacgcccg ctgcacgcac ctgggctgca cgccagactg
     2461 gaagcccagc gagaacaaat tcaaatgtcc ttgccacggc agtggctacg acagtgaagg
     2521 gatcaatttc gaaggccctg cgccgcgtcc gatggatcgc gcgcacattg aattggcgcc
     2581 tgacggccag gttctggtgg acacatcgaa gttgtattcg tggcccaagg gccagccgac
     2641 tcacttcaac gatccgggag cgttcctgac gggtgtgtag ctggaaagca gattcctcgc
     2701 gcggacagca tcgcgctcgg aatgacaaga aatttgagat tggtactcat ccatggctga
     2761 cgaaaacaac ggcaagaacg gaaaagtcgg gctggtcgag cgcgtaacga ctggggtgaa
     2821 agatgacatc caggcgctca aaacggatat ccccaccaag gccaaggaac agatcgaggg
     2881 gctgaaggat ccgaccaaga cgcaggtcta cacgtcgatc tttcgtcaca agcacgatga
     2941 cacgccgcgc aaccgcgcgc tgggcgtgct gtcgaacgtc ttcctgcatc tgcatccggc
     3001 caagatcaac cgcgacgcgg tgcgctacag ctacacctgg ggcatgggcg ggatcacttt
     3061 ttatctgttc atcgtcctca cctacaccgg cgtgctgctg atgttctact accaccccag
     3121 caaggtgcag gcgtttcgcg acgtgctcta tttggagcac gacgtgccgt tcggcaagat
     3181 tctgcgcaac atgcatcgct gggcagcgca cctgatggtt attgccgtct ggttgcacat
     3241 gttccgcgta ttcctgaccg gctcgtacaa aaaaccccgc gagtttaact ggaatatcgg
     3301 ggtactgcta ctggtgttca cgctgctgct ttcgtttacc ggataccttt tgcccgacga
     3361 tcaactcgga ttctgggccg tgactgtcgg cacgaacatg gcacgcgcca ctccgctgct
     3421 ggggcatgaa gggccgttcg gcccgcagct ggggatgacc ccatataacg acgttcggtt
     3481 tggattgctg ggcggctcga ttgtcgacgc gaacgcgctg ctgcggtcct acatctggca
     3541 ctgcattggt attccgattg tcgcgtcggt gctgatgatc gtgcatttct ggcgagtgag
     3601 aaaagatggc ggcatatcag gcccggcgca ggtagtgctg gcggctgagg cggacaagcc
     3661 gcggcgaggg ccgaaggcgg cgggagagta aataagctgt gtaaagacag ccgccctcgg
     3721 ctgtccggtc gagcgcagct cgatttttta ccagtgcatc aggaaaccgc cgggcttcac
     3781 ccgggacgac aaccgagggc ggctgtcgct acatggtcgc tggagactct atggattggc
     3841 gacaactttg ggagatctgt tcggcgcccg acaacgttcc aatcgtcggc ctgattcctc
     3901 tgctcatctt ctacatttac ctggcgtgga aacaggctca tgccaacgat caactgatcg
     3961 cgcaactcga agcctcgccg gagatggcga agacgcatca tcgcaagacc tggccgttca
     4021 agccgggatg gcagaaagaa gttcacgtct ggccgttcct gttgcgcatc gagtttctcg
     4081 ccgcaatcat cgtcaccatc attctgatgg tctggtcgat cacactgaat gcgccgctgg
     4141 aagagcctgc caatcccaac ctcacgatga atcctgcaaa ggctccatgg tactttctcg
     4201 gcctgcagga gatgctcgtc tatttcgatc cctggatcgc gggcgtggtc atgccgacca
     4261 tgatcatcat tggcctgatg gtgattccct acatcgacac gaatcccctg ggcagcggct
     4321 attacacctg gaagcagcga cgcttctcga tcgcgacatt tctgtttggc ttcatcattc
     4381 tgtgggtcgc gatgatcatc atcggaacct ttatccgcgg accaggttgg caatggttct
     4441 ggccggggca gacgtgggat cacaaccggc tgatctacga ggtgaaccgc gatttgcccg
     4501 atgaggtgcc gcgctggctg ggcggcccga tcacgtccaa ttgggcaaag ggagttttcg
     4561 gcgcgattgt ggtgggcgga tattacctga ttggcggcgg cgcggtgtat tcgctcttcc
     4621 gccggaaaat gcagaaagat tttcagcgca tgagcctgct gcagttttcg atcatggcat
     4681 ctctgtggct gacgatggtc gcactgccca tcaagatggg gttgcgcctt gtatggcaca
     4741 tcaagtacgt gtggattaca ccgtggttca acgtgtgatc agattgcaga agtgagattc
     4801 cagattgcag atttgtattg tcatgctgag cggagccgaa gtcctgcggc gcaggatttc
     4861 ggcgcagtcc tacccacaat gctgtgccgg gggtagagat tcctcgcttt gctcggaatg
     4921 acaaagcttt tggggaatat tttggtcgga ggaatcggtg cccgaaaaac ccactcccga
     4981 gcaggatccg atcaccagca agtcctacgc cgcccactat gccatcgcca cggtgattct
     5041 ggtcattacg ctgttctggg ccttgtggga cgaagcctgg ggacagcgtc cgtggaagga
     5101 gttccaggag cagtggaaga cgcgctacac ggcctttctg aacacggcga agtccaagtc
     5161 gcagacctcg gaaaaggatg tcgagaagaa ctcggattac gcgacgctct atgccgcatg
     5221 gaagaaggcc agcgacgact cggctcctca ggcgaaaaaa gttcgcgacg aactgaatga
     5281 tgcgagcgcg cggctactgg cggtgcagag cgtatttacc gaccggcgtg catatgtaaa
     5341 cgctctcacg tacgaattgg aaaccagcga aagccaatca tcaaaggcca gcaagcagaa
     5401 agatatcgac gcttataaag ctgagcaggc aacggttgaa tttcccgatg gcagcaagaa
     5461 gaaattcaac ttccagcagc tggaggagac ctacaacgag attcgcgacg agcgcaccaa
     5521 gctcagcctg gaactgggcg acgtccttaa accggcgaca gatgcaaaga acaagttgga
     5581 tgaatacgtc accgatcata tggtggacct aacgccggca cagatcgacg gcctcaagaa
     5641 gaaagctgca gactgggatc ctgaaatcaa gcaaattaat gtggcggaag ccaacatcgt
     5701 ggaccggtgc gaatcctgcc acatgagctc gcgcgagccg ctgaagatta cagctgcctc
     5761 catgaccctc aagggggaaa agaagcccga cgagtatgcg caggcgcttg tcagccacac
     5821 cgagccggag ctgctgcaga ttca
//
LOCUS       DASPUL010000610         2998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1617350, whole genome shotgun sequence.
ACCESSION   DASPUL010000610 DASPUL010000000
VERSION     DASPUL010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617350"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            530..2413
                     /locus_tag="VEJ00_14105"
     CDS             530..2413
                     /locus_tag="VEJ00_14105"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXX02344.1"
                     /translation="MNRRAFLKAIGSAALLPMLPRRLLADAAPAASGFRRRRPSDATW
                     PSQADWKRLNDAVGGTLIRVDSPISVLSTDPDGAAANQLAANLQNPYYIGEQPGLTQS
                     SGWLDAWASKPSVYAVAARNANDIAAAVNFARDNDLRLVVKGGGHSYQGTSNAPDSLL
                     IWTRHMNDIAMHDAFVPSGCQHTMQPQSAVTFGTGILGMQAYDVVTTKAGKYIQGGGC
                     LTVGLAGLIQGGGFGSYSKRYGTAAGSLLEAEVVTADGQIRIANACTNPDLFWALKGG
                     GGGTFGVVSRMTVRAHELPEFFGAANITIKASSDDAYRGLIRDIVAFYRDHLFNEHWG
                     EQVHVRPDNTLEVRMASAGLSTEQARQVWQPFLDSVKHSSQCSIKGITTIGSIPARHW
                     WDPQWWKEHWPEIAFPRDGAMHAIIDDVLLHVMHQPVFTFDPRPGASPNNAWWVGNTG
                     EASIFWWAYQSLWLPESLLETGSQQRLADALFAASRYAGIELHFNKGLAGAPPDAIAA
                     AKDTATNPSVLSAFALAIVGDGQGPSYPGIAGHEPSVETGRKAAARIDQCMSELRAVA
                     PNGGAYVSESNYFEKNFQQAYWGSNYPRLLEIKKKYDPSGLFTVHNGVGSEEWSADGF
                     TKL"
     gene            complement(2667..>2998)
                     /locus_tag="VEJ00_14110"
     CDS             complement(2667..>2998)
                     /locus_tag="VEJ00_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXX02345.1"
                     /translation="PLNLVKEMFFTGASVKPADAEKWGLLNHLVSAETLEQFTYDLAR
                     LVATKAPLAVSVVKEQLRILAAADPLTPAAFERIEELRQKVVQSSDYAEGIRAFRDKR
                     KPVFVGQ"
BASE COUNT          574 a          938 c          928 g          558 t
ORIGIN      
        1 gagatcctcc ccgagctggg cagtctcagc cgctagcagc ccgttgagaa agccccatct
       61 cgccattccg tcacgagtgg cggtgtaggc catgccggtc ggcggtatgt gttgatagac
      121 gtcgatgcga ctggtcgcgg ctgaacccgc cacgcgagcc acaacagcgc gaggaacact
      181 aggccacggg ctccctgcag gcttcgcggc gttccgacac cgcacagcgc cagcatgagt
      241 atcccgagat cgcacgccgc ggctcgcatg aggtgcgcct tcgagacgtt caccacccca
      301 cgtaacgagg tgcgcctgcc gccaccggtc tcgcacgtgt gcgcgaagct gcgctcgacc
      361 ttctcgctcc gccagcgtcc gagtcggcgc ccgcggctac cctggatgcg tcgccgattg
      421 gcgtacacgg cctcctgttg tccgggttcc ttgtcctgcc gagagagcgg cgtccgcgcg
      481 cgcattgtga tatccacggt cgtgtgaaga attctaaaac gcggatccaa tgaacagaag
      541 agcattcctg aaagcaattg gatcggcggc gctgctgccg atgttgccgc gccggctcct
      601 cgctgatgcc gctccggccg ctagcggatt ccgtcgtcgc cgtccgtccg atgccacgtg
      661 gccatcgcag gccgactgga agcgcctgaa cgatgcggtc ggcggaactt tgattcgcgt
      721 cgactctccc atctccgtac tcagcaccga tcccgatggc gccgcagcga atcagctcgc
      781 ggcgaattta caaaatccct actacatcgg cgagcagccc gggctcacgc aaagttccgg
      841 atggctcgat gcgtgggcga gcaagccgag cgtctatgcg gtcgcggcgc gcaatgccaa
      901 tgacatcgcg gcggcagtta acttcgcgcg cgacaatgac ttgcgcctgg tggtaaaggg
      961 cggcggtcat agctatcagg gcacgtccaa tgcgcctgat tcgcttctca tctggacgcg
     1021 ccacatgaac gatatcgcga tgcatgacgc gttcgttccg agcggatgcc agcacacgat
     1081 gcagccgcag tcggcggtta cgttcggcac cggcatcctt ggaatgcagg cgtacgacgt
     1141 ggttacaacc aaagcgggga aatacatcca gggaggcgga tgcctgaccg tcggcctcgc
     1201 gggtctgatc cagggcggcg gctttggaag ctactccaag cgctatggca ccgccgctgg
     1261 cagcttgctc gaagccgaag tcgtcaccgc cgacggccaa attcgaatcg cgaatgcgtg
     1321 caccaatccc gatctgttct gggctttgaa aggcggcgga ggtggcacct tcggtgtcgt
     1381 gagcaggatg acggtgcgcg cgcacgagct gcccgagttt ttcggcgcag caaacatcac
     1441 gatcaaagcg tcctccgatg atgcgtaccg cggcctcatc cgcgacatcg tcgccttcta
     1501 tcgcgatcat ctcttcaacg agcattgggg tgagcaagtc catgtgaggc ccgacaacac
     1561 cctggaggtc agaatggctt cggcgggctt gagtaccgag caagcaaggc aagtctggca
     1621 gccgtttctg gattcggtga agcattcgtc acaatgctcg atcaaaggca tcacgaccat
     1681 cgggagcata ccggcgcggc actggtggga tccgcagtgg tggaaggagc actggccgga
     1741 gatcgcgttt ccgcgcgacg gcgcgatgca cgcgatcatc gacgacgtcc tgctgcatgt
     1801 gatgcatcag cccgttttca cattcgaccc gcggcccggt gcgagtccca acaacgcgtg
     1861 gtgggtcgga aacaccggcg aggcatcgat cttctggtgg gcatatcagt cgctatggct
     1921 gcccgaatcg ctactcgaga ctggctcgca gcagcgccta gccgacgcgc tattcgcagc
     1981 gtcccgctat gcgggaatcg aactccactt caacaagggt cttgccggag cacctcccga
     2041 tgcgatcgcg gccgcgaagg acaccgccac gaatccatcg gtgctgtcag catttgcgct
     2101 ggcgatcgtt ggcgatgggc aagggccgtc gtatcccggc atcgctggcc atgagccatc
     2161 ggtcgagact gggcgcaaag ccgccgcgcg catcgatcag tgcatgagtg agcttcgcgc
     2221 ggtcgcgccc aacggcggag cctacgtgag cgagagcaat tacttcgaga agaactttca
     2281 gcaagcatac tggggcagca actatccgcg cctcctcgag atcaagaaga aatacgatcc
     2341 ttcaggactg ttcaccgttc acaatggagt agggtctgag gagtggagcg ccgacggctt
     2401 cactaagctc tgattcgctc cgctagctct cggcgcactt cctattgctc ggcaattcgc
     2461 ccgacggaga ctgagcagct gctgtcatgg ccgccaggat gtcatgcggc cagctcgcag
     2521 cggataaccc gtcttcggcg acgaggcgta gctcctgctt ggtttcgatg tcgctcgcaa
     2581 ggtggatgtg aacgcaagaa gcggagcaat tgcctcccgc gggtcacgaa tcatcgcact
     2641 cagagctgag agcttctccc tgttagctat tgcccaacaa agacgggttt ccgcttgtcg
     2701 cggaaggcac gaatcccttc ggcatagtcg gagctttgca cgaccttttg gcggagctcc
     2761 tcgatgcgct cgaaggcggc cggcgtgagg gggtcggccg ccgccaggat ccgcaactgc
     2821 tccttgacga cagacacggc gagcggcgcc ttggtcgcaa ccaggcgcgc gaggtcgtag
     2881 gtgaactgtt cgagcgtctc tgccgacacc aaatggttga gaagccccca cttttccgcg
     2941 tcggccggtt tcaccgaagc cccggtaaag aacatctcct tcacgagatt cagcgggg
//
LOCUS       DASPUL010000611         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1343978, whole genome shotgun sequence.
ACCESSION   DASPUL010000611 DASPUL010000000
VERSION     DASPUL010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343978"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1957
                     /locus_tag="VEJ00_14115"
     CDS             <1..1957
                     /locus_tag="VEJ00_14115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02346.1"
                     /translation="LVANMKQGIGYESTPLVSFILGGSANNGPGWYNFEKSDVSPRIS
                     LAYSPRPKEGLLRKIFGDNDATVLRGGFSKVYDRAGMQLLSTFDANPPGGLGATLQNP
                     CCSSQLPVGVAGTPGTAYDTAAGVPRVTNMNVVPTVSQFTGNTFLTPAPPGAFPQTPS
                     ATLQAITWGVDQSQKTPYAYAFDFSVGRELPRRMSLQLSYVGRLGRNLLTQRDLRQPL
                     DIVDPKTGIDYYAAATDLARIAFQHLNNSFKTFADYVNFVNGSISDAVIPTSQYWHDM
                     LPPLQPGATQYQSFANFAGITPLTGNGGNASLIQAIYDLYYDPFLSYAGNEVVGIGNA
                     DIYGGLNDDLGNLYYFCPTPGCTTLSGQPGNYLSNQATSMFAWSSIGKSNYNALQATL
                     RKQFSGGVQFDLNYTYSKSIDLTSSAARLGWSACCNVGAPGTRLVNAFDPNGRRGVSD
                     FDTTHQINANWIAELPFGRGKHFAANAKGAADAFIGGWQLSGLARWTSGFPFTVDNGN
                     FWPTNWDEQGIAQMVTRPRIGHHRDPGTGAVSAFASPATAFSDFAHPFPGQSGSRNVI
                     RGDGYAGWDMALSKRWNMPFEGQSLQFRWEVFNVPNLHRFNVASGLGTSACTCIASLQ
                     QSTQSFGDYSGLLTQPREMQFALRYEF"
     gene            2012..>2911
                     /locus_tag="VEJ00_14120"
     CDS             2012..>2911
                     /locus_tag="VEJ00_14120"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXX02347.1"
                     /translation="MTTLAHKLRLVDYFTLGFGVMVGTAWLVVMDDILNRGGPLGAIL
                     GFTAGALILLPIGFVYGQLVRTIPDAAGEAAYVARFFPPVVSFATGWMMFLSYFLTCP
                     FEALAAGRITGYLFPSLNTIELYRVGGYPVYLPHLLLGLTLTVLLTGLNYRGIQASAR
                     VSKTTTFTFLSLVIVFALAGAGHGSVSNFHPAFSHTPLISILLVWQVVPWLLSGFESV
                     GKIAEEASPGFGGRNFSVAILLTIISGLVFFWIVIGAVSCVAPWKSLNSNQQFPTATA
                     FERALHARWIVVLIMGSALVALVQ"
BASE COUNT          546 a          882 c          830 g          653 t
ORIGIN      
        1 actcgtcgcg aacatgaaac agggcatcgg gtatgaatca accccgctgg tctcgtttat
       61 cctcggaggg tcagcgaaca acggaccggg ctggtacaac ttcgagaaat ccgatgtttc
      121 gccacgaatc tcgctggctt attcgccacg tccaaaagaa ggtttgctca ggaagatttt
      181 cggggacaat gatgcgacgg tcctgcgcgg cggattcagc aaagtctacg atcgcgcggg
      241 aatgcagctg ctgagcacct tcgacgccaa cccgcctggc ggcttgggcg ccacactgca
      301 gaatccctgc tgcagttcgc agctgcccgt gggagtcgca ggaacgccgg gcaccgccta
      361 cgacacggcc gctggcgtgc cgcgggtaac caacatgaat gtggttccta ctgtcagcca
      421 gtttacgggg aacacattcc tgactcccgc acccccgggc gcgtttccgc agacaccatc
      481 cgctactctt caggcgatca cctggggagt cgaccagtcg cagaagactc cttatgctta
      541 cgcctttgat ttttctgtgg gtcgtgaatt gccaagaaga atgtctctgc agctttctta
      601 cgttggccgc ctgggccgca atctcttgac ccagcgcgat ctgcgccagc cgctcgacat
      661 cgtcgatccg aagaccggaa ttgactacta cgccgcggcg accgatctgg cccggatcgc
      721 tttccagcac ctcaataact cgttcaagac ctttgccgat tacgtgaatt tcgtcaacgg
      781 ctcgatcagt gacgccgtga ttcctacttc gcagtactgg catgacatgc tgccgccgct
      841 gcagccgggt gcgacacaat accagagctt cgccaacttt gccggaatca ctcctctcac
      901 gggcaatggc gggaacgcca gcctgatcca ggcgatctac gatctttact acgatccatt
      961 tctctcttat gccggcaacg aagtggtggg tatcgggaat gccgatatct acggcggttt
     1021 gaacgatgac ctgggcaacc tgtattactt ctgcccaact ccaggctgca ctaccctgtc
     1081 cggccagccg ggcaactact tgagcaacca ggcgaccagc atgtttgcct ggagctccat
     1141 cggaaagtcg aactacaacg ccctgcaagc cacgctcagg aaacagttca gcggcggcgt
     1201 acagttcgac ctcaactaca cctactccaa gtccattgat ctcacttcgt ccgcggcgcg
     1261 gctgggttgg tccgcgtgct gcaatgtggg agctccgggg acccgcctgg tgaacgcttt
     1321 tgatccgaac ggccgacggg gagtttccga tttcgatacc actcaccaga ttaatgccaa
     1381 ctggattgcg gagttgccct tcggcagagg aaagcacttc gcggccaacg cgaaaggcgc
     1441 ggccgacgcc ttcatcggcg gctggcagct ttccggattg gcccgctgga ccagcggctt
     1501 tcccttcacc gtggacaatg ggaacttctg gcccaccaac tgggacgaac agggcattgc
     1561 gcagatggta accagaccga ggatcgggca ccatcgcgat cccgggactg gagccgtcag
     1621 cgccttcgcg agccccgcga cggcattcag cgactttgcc cacccgtttc cgggccaatc
     1681 cggatcgcgc aatgtgatcc gcggagatgg ctatgctggc tgggacatgg cgctgagcaa
     1741 gcgctggaac atgccctttg aaggccaaag cctgcagttc cgctgggagg tctttaacgt
     1801 cccaaacctg catcgcttca acgtcgcatc gggcctggga acctccgcct gtacctgcat
     1861 tgcgtcgctg caacagtcaa cccaatcatt cggcgattac tcgggccttt tgacgcagcc
     1921 tcgcgagatg cagtttgcac tgcgatacga gttctaaacg ttcctcgtgt agaggcgtgg
     1981 catgtgatgc ggtcggacgc tctaggcaga aatgacgact ctcgcgcaca aactgcggct
     2041 ggtggactac tttacgctcg gcttcggcgt gatggtaggc acggcctggc tggtagtgat
     2101 ggacgacatc ctgaatcgtg gcggcccgct cggcgcgatt ctgggattta ccgccggcgc
     2161 gctgattctg ctccctatcg ggttcgttta tgggcagctt gtcaggacga ttcccgatgc
     2221 tgcgggcgag gccgcatacg ttgcgagatt tttcccgccg gtcgtcagct tcgcgaccgg
     2281 ctggatgatg ttcctgagct atttcctgac ctgccctttt gaggccctgg cggcgggaag
     2341 gatcaccggc tatctgtttc cgtctcttaa cacgatcgag ttgtaccgcg ttggcggata
     2401 ccctgtgtat ctgccccacc tgctcctggg tctgactctc acggtcctct tgacaggact
     2461 caactaccgc ggcattcagg caagcgccag ggtaagcaag acgacaactt ttactttctt
     2521 gtcccttgtc atcgtttttg cgctcgcggg agcgggccac ggctcggttt cgaacttcca
     2581 tcctgccttt agccacacgc ccttgatctc cattctgctg gtttggcagg tcgtgccgtg
     2641 gctgctgtcg ggctttgaat ctgtggggaa gatcgcggag gaagccagtc ccggatttgg
     2701 ggggcgaaat ttctccgtcg cgatcctgct gactatcatc tctggtctcg tattcttttg
     2761 gattgttatc ggtgcggtgt cctgcgtagc cccatggaag agcttgaata gcaaccagca
     2821 attcccgaca gcgactgcgt tcgagcgagc cttgcatgcc cgctggattg tggtcctgat
     2881 catgggatcg gcgctggtcg cactggttca g
//
LOCUS       DASPUL010000612         5644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1619041, whole genome shotgun sequence.
ACCESSION   DASPUL010000612 DASPUL010000000
VERSION     DASPUL010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619041"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VEJ00_14125"
     CDS             complement(<1..531)
                     /locus_tag="VEJ00_14125"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX02348.1"
                     /translation="MFPPLSYARKPITIRRLQFFSRAAGLVLLTGLSAIGAAQINADG
                     ANESPDAAALLRNRQFPAAVAAYRRILRREPHNEKAELGLAAAYYGLYNYDQTRRVLR
                     EAATQHPKSAAALVELGKLDIHLLHYDDAIVELKRAVQRDPASAAAHEQLGVAYQAKG
                     DGDKALPEFNEAIRLAP"
     gene            complement(621..3953)
                     /locus_tag="VEJ00_14130"
     CDS             complement(621..3953)
                     /locus_tag="VEJ00_14130"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXX02349.1"
                     /translation="MRAARIVFVAVLVIASTLCFAQTLERGAVHGTVYDSSHAVIATA
                     KITLSNPSTGFHRELNTTPDGGYDFEAVPPGEYTLVAEAQGFAITTVTGITVNVGTSV
                     PLDVSMPLKSAQEQVTVSANIEAVDTSTAGVSQLMDAKSLETLPFPGRDYRDLAQLTP
                     SAQVVPGLRGGIRLGGQQSDYSGLVIDGADTTNNYFGENFGSLETKNLTVPIEAVQEF
                     QVVTNGFAPEFGRATGGLLNVVTKSGTNNVHGEAHEYYRGDSLSKNDALGNPSAIKWQ
                     NQFGGSIGFPIRKDRQFLFLSTDIQRNTGPLTTNLCHGDPACENDAGPIIPVAIPGQS
                     EVLPPACANVTPGVTNLLPGCYGVANLGALEGGHTQFQDFFTLLGHYDYQITQANHFS
                     IRGLFTRNHTRGFTGGHGQSETFDSILDTEDFVNQGPSGVFALTTARGRKVNEIRALV
                     QYEGRKRHPIFSGAPEISIAGTGEFGQRFYLPANNDNGKLQIQDNFSYSFSKHDMKFG
                     GDTDSFVDRKDVFAGWSAGRYNFASLADFDNLNPDAYFQGFGLNGIDPFTANTLKPNY
                     QNGLGLYWQDKWQAKPNLTVTYGLRWDGTWNPQPQTPIPGQEVYTGVGPLGHGTKISP
                     VPQKTPNDFKQWGPRVGIAWSLGAAQHPTVVRAAWGLYYAQTPTIFFPQVSNGGGSKS
                     TTLFCTPSFGCAPGGGFGAPFQVPYTYPSTLSQTSAQLCGLSAPQPAIGCPAIEYADP
                     ALRNPRVSNVTVGVEHQFNNEWSVSVNYVYMYSSHLKTGGFSTSNWIRNLVPAGTDQF
                     GRSVLEAVPASGACPADAQVFPGEPLPLDCSLTNFLGALSLASFSHGNYDEVVAGVNK
                     RFGHRFEWFANYTWSRNFSNDSSERDTDTFFGTQDPFNINIDYGRNGLDITHQFKSGV
                     SVQLPWSLNWSSNFIAHSGLPYPAYTATANQFSTDDADLNGDAVINQFSNNDRPTIQI
                     GNGPLQLLSRYPARQPDFFTWDMRLSKDFKFGDRYDLRLIADIFNLTNRGNLYSNPDN
                     SGFVGLSNCAPTAPLNGTPVVGFTCDPLTAVPKQGNVYTVPTLVNGPFKGVPLQFNYG
                     VHDEISPGSTAFAAQLGVRFSF"
     gene            complement(4135..>5644)
                     /locus_tag="VEJ00_14135"
     CDS             complement(4135..>5644)
                     /locus_tag="VEJ00_14135"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HXX02350.1"
                     /translation="RIDDPMPKGALPVKDSPKYWNRLYHQKADGTFEDITEKSGLAGE
                     GYGMGVAVGDYDNDGREDLYVTGFPSNHLYHNEGNCIFEDVTDSAHVGGSGWSASAAF
                     VDVDNDGLLDLVVTRYLVWSFDNNPYCGEHAPGHRGYCSPDIFTGISPILFHNDGNGH
                     FSDWSQKSGLSKLEGKGLGVAFADMDHDGRMDIVVANDAVRQFLLHNEGGGLFKDIAV
                     DAGTAVNEDGRVFSGMGVDFADYDNDGNPDIVITNLSDQKYALYRNTGRGSFTYETGP
                     SGLGLITRLYAGWGVKFFDYDNDGWKDLFIAQGHVMDTIQLTYPHLRYLQPPLLLHNQ
                     AGRKFSDVSAQSGEIFKQKWAARGLAIGDLGNRGALDVVVTTNNGPAYVLRNQGGDRN
                     HWLTLQLEGRKSNRDGIGAEVKIVSASGTAQYATVTTAGSYLSASDRRVHFGLGADAS
                     VKSIEIRWPSGIVQRLENVNANQILNASEPDQSAADAVKTPAKTSNDSNPQK"
BASE COUNT         1124 a         1636 c         1711 g         1173 t
ORIGIN      
        1 cggagccagg cgaatcgctt cgttgaattc gggcagagcc ttgtccccgt cgcccttggc
       61 ttgatacgcc acgccgagct gctcgtgagc cgcggccgat gcgggatctc gctgcaccgc
      121 ccgcttcaat tccacgatgg cgtcgtcgta gtgcagcagg tggatgtcga gcttgccaag
      181 ttccaccaat gccgcagcac tcttcggatg ctgtgtcgcc gcttcccgga gcacgcgccg
      241 tgtctggtcg tagttgtaaa ggccgtagta ggccgcggcc agcccgagtt ctgccttttc
      301 gttgtgcggc tcgcgtcgga ggatcctccg gtaggcggcc accgcagccg ggaactggcg
      361 attgcgcagc agggccgccg catccggcga ctcgttcgcg ccatccgcat ttatctgggc
      421 cgcaccgatc gccgacaacc cggtcagcag cacaagccca gcggcgcggc tgaagaattg
      481 aaggcgccga atcgtgattg gcttacgagc gtagctcagc ggcggaaaca tgcaggaggt
      541 agttgctcgc ctgattctcc aacaaaaatc aggccgcctg ccagaggcaa ggcggcccga
      601 cgtctcgtga tgcaagcccg tcagaagctg aagcgcaccc ccagctgcgc ggcaaaggcc
      661 gtgctgccgg gagagatttc gtcatgcacg ccgtagttga actgaagggg aacgcctttg
      721 aaggggccat ttaccagggt aggaacggtg tagacattgc cctgtttcgg tacggcagtg
      781 agcggatcac aggtgaaacc aactacgggc gttccgttaa gaggcgcggt gggagcgcaa
      841 ttcgacaatc cgacgaatcc actattgtcc ggattcgagt acaaattgcc acggttggtc
      901 aggttgaaga tatcggcaat caggcgcagg tcgtagcggt cgccgaactt gaagtctttg
      961 ctcagacgca tatcccaggt gaagaaatcc ggctgacgcg ccggatagcg gctaagaagt
     1021 tggagcggcc cgttgccgat ctggatggtc ggccggtcat tgttcgagaa ctggttgatc
     1081 accgcatcgc cattgaggtc cgcatcgtcg gtcgagaact ggttcgctgt cgccgtatag
     1141 gccgggtagg gaaggccgct gtgggcgatg aagttgctgc tccagttgag actccatggc
     1201 agttgcacgc tcactcccga tttgaactga tgcgtgatat ccaggccgtt ccgcccgtag
     1261 tcaatattga tgttgaacgg atcctgcgtg ccgaagaagg tgtcggtatc gcgttcgctc
     1321 gagtcgttgc tgaaattcct cgaccaggtg tagttagcga accactcgaa acggtggcca
     1381 aaacgcttgt tcacacccgc gacaacctcg tcatagttac catgactgaa gctcgcgaga
     1441 gacagtgctc ctaagaagtt agttagcgag cagtccaatg gcagcggctc gccgggaaag
     1501 acctgggcgt ccgctgggca agcgcctgag gccggcacgg cttcaaggac actgcggccg
     1561 aactggtcgg ttccggcagg caccaggttt ctgatccagt tcgatgtgct gaatcctccg
     1621 gtcttcagat gcgatgagta catgtagaca tagttcactg agacggacca ctcattgttg
     1681 aactggtgct ctacacccac agtcacgttg gaaacgcgcg gattgcgcag ggcggggtcg
     1741 gcgtattcga tggcaggaca gccgatcgca ggctgaggtg cgctcaggcc gcagagctgg
     1801 gcggaagtct gggagagcgt gctcgggtag gtgtagggaa cctggaaggg cgcgccaaaa
     1861 ccacctcccg gagcacagcc aaacgatgga gtgcaaaaca acgtggtcga cttcgagccg
     1921 ccgccattgg aaacctgtgg gaagaaaatc gtcggggtct gggcgtagta caaaccccac
     1981 gccgcccgca ctaccgtagg atgctgggct gcgccaaggc tccaggcaat gccgacacgc
     2041 ggaccccatt gcttgaagtc gttgggggtt ttctgcggaa cgggcgatat cttggtgccg
     2101 tgaccgagcg gacccacgcc ggtgtaaacc tcttgccccg gaatcggcgt ttgcggctgg
     2161 gggttccacg taccatccca gcgcaagccg taagtcacgg tcaaattcgg cttagcctgc
     2221 cacttgtcct gccagtagag tcccagcccg ttctgatagt tgggtttgag cgtgttggcc
     2281 gtaaagggat cgatcccatt caggccgaaa ccctgaaagt aggcgtcggg gttcaggttg
     2341 tcgaagtccg ccaagctagc aaagttgtac cggcccgcgc tccatccggc gaacacgtcc
     2401 tttcgatcca cgaacgagtc cgtatctccc ccgaatttca tgtcgtgctt gctgaaggag
     2461 taggagaagt tgtcctggat ctggagcttg ccgttgtcgt tgttggcggg caggtagaag
     2521 cgctgtccga attcgccggt gccggcgatg gaaatctcag gtgcgccgct gaagatcgga
     2581 tgccgctttc ggccctcgta ctgtaccaga gcccgaatct cattcacctt ccgcccccgc
     2641 gctgtcgtta gggcaaagac tccgctcggc ccctggttta cgaagtcctc cgtgtccaga
     2701 atcgagtcga atgtctccga ctgtccgtgg ccgcccgtga atccgcgggt gtggttgcgg
     2761 gtgaacaggc cccggatgct gaagtgattc gcttgcgtga tctgatagtc gtagtgtccc
     2821 agcagggtaa agaaatcctg gaattgggta tggccgcctt ccagagcgcc aaggttcgcc
     2881 acaccgtagc aaccagggag aaggtttgta acgccggggg taacattagc gcaggctggc
     2941 ggcagaactt ccgattggcc ggggatcgcg accggaatga tggggcccgc gtcgttctcg
     3001 cacgccggat ctccgtggca gagattcgtc gtcagaggtc cggtgttgcg ctgaatgtcg
     3061 gtggagagaa agagaaactg acgatccttg cggatgggaa agccaatcga tccgccgaac
     3121 tgattttgcc atttgatagc ggagggattg cccagcgcgt cgttcttgct caaactgtcg
     3181 ccgcgataat attcgtgcgc ttcgccgtga acgttgttcg ttccggattt agtgaccacg
     3241 ttcagcaatc cgccagtggc gcggccaaac tccggagcga acccgttggt gaccacctgg
     3301 aattcctgca ctgcctcgat gggtactgtc aggtttttgg tttcgagcga gccgaagttc
     3361 tcgccgaagt aattgttggt cgtatcggcg ccatcaatca ccaggccgct gtaatcactc
     3421 tgctgtccac cgagacggat gccgccgcgc aagcccggca ccacctgcgc cgacggcgtc
     3481 aactgcgcga gatcccgata atcccggccc ggaaagggca aagtctcgag actcttggcg
     3541 tccatgagct ggctgacgcc ggcggtcgag gtatcgaccg cctcgatatt ggcactcacc
     3601 gtcacctgct cctgcgccga cttgagcggc atgctgacgt ccagcggaac cgacgttccg
     3661 acgttaacgg taatgccggt cacggtcgtg atggcaaagc cctgcgcttc ggcgaccagc
     3721 gtgtattcac ccggcgggac ggcctcgaaa tcgtacccgc cgtctggagt ggtgttcaat
     3781 tcccggtgaa aaccggtgga cgggttgctc aacgtaatct tggccgtggc gatcacggca
     3841 tgggaggaat cgtaaacggt gccgtgaacc gcgccccgct ccagcgtctg ggcgaagcac
     3901 aaagtactgg ctataaccag caccgcgacg aaaactatcc ttgcagcgcg catctcccgc
     3961 ctccaagcgc aaaacgatct gaccgctttt ggtttgactc cggctacaaa acgcccctgc
     4021 gtcccgagcg ggacgataac aggaaagcaa gaccgctaat ataaaagcat taagcgctgc
     4081 attagactgc ccggccccgt gtggtgtcaa gtctattttt cgtagcgcca aggttcattt
     4141 ctgtggattg gagtcgtttg aggttttcgc tggagtttta acagcgtcgg ctgctgactg
     4201 gtcgggttcg ctcgcgttga gaatctgatt cgcattcaca ttctcgaggc gctgcacgat
     4261 tccgctgggc cagcgaatct cgatcgactt gacggaagca tccgcgccga gcccgaagtg
     4321 cacacgccgg tcgctggcag aaagataact gccggccgtc gtcacggtcg catactgcgc
     4381 cgtgcctgac gccgagacga tcttcacctc ggctccaatc ccgtcgcgat tgctcttgcg
     4441 tccttcgagt tgcaacgtca gccagtggtt gcgatcgccg ccctggttgc gcagaacgta
     4501 agccggcccg ttattggtag tgacgactac atcgagcgca ccgcgattgc cgagatctcc
     4561 gatcgctaac ccgcgcgccg cccacttctg tttgaagatt tcgcccgact gcgctgaaac
     4621 atcgctgaac tttctgccgg cctggttgtg gagcagcagc ggcggctgca gatagcgcag
     4681 atgaggatag gtcagctgga tcgtatccat cacgtgtcct tgcgcgatga acagatcctt
     4741 ccagccgtcg ttatcgtagt cgaaaaactt gacgccccac ccggcataca gacgcgtgat
     4801 cagcccaagg cctgaaggtc cggtctcgta ggtgaacgat ccgcggcctg tgttgcgata
     4861 gagcgcatat ttctggtcgg agagattggt gatcacgata tcgggattgc cgtcgttgtc
     4921 atagtcggca aaatcgactc ccatgcccga aaacacgcgc ccgtcttcgt tcacggccgt
     4981 acctgcgtcg acggcgatgt ctttgaacag tccgccgccc tcgttgtgca acagaaactg
     5041 cctgacggca tcgtttgcaa cgacaatgtc catccgcccg tcgtggtcca tgtcggcgaa
     5101 ggcaacgccc aggcctttgc cttcgagctt cgaaagtccc gatttctgag accaatcgct
     5161 gaagtggccg ttgccatcgt tgtgaaagag aatcggcgag atgccagtaa aaatgtcggg
     5221 gctgcaatat ccacgatggc cgggcgcatg ctcgccgcaa tatggattgt tgtcgaacga
     5281 ccacacgaga tagcgcgtca cgacgagatc gagcagaccg tcgttgtcca cgtcgacaaa
     5341 cgccgcgctc gccgaccatc cggacccacc gacatgcgcg gaatcggtca catcctcgaa
     5401 tatgcagttg ccctcgttgt gatacaggtg attgctcggg aaacctgtca cgtacaaatc
     5461 ttcgcggcca tcgttgtcat agtcgccgac ggcaacgccc attccgtagc cttctccggc
     5521 gaggccggat ttctctgtga tgtcttcgaa ggtcccgtcg gccttctgat ggtaaaggcg
     5581 attccagtac ttcggcgaat ccttgacggg cagcgcgccc ttgggcatgg gatcgtcaat
     5641 gcga
//
LOCUS       DASPUL010000613         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1893328, whole genome shotgun sequence.
ACCESSION   DASPUL010000613 DASPUL010000000
VERSION     DASPUL010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893328"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..995
                     /locus_tag="VEJ00_14140"
     CDS             420..995
                     /locus_tag="VEJ00_14140"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXX02351.1"
                     /translation="MTSDEALMLEFQAGSRQAFEELFARYHEPLSGFFRRRLSNGERA
                     EDLAQETFLAVIRATSRYEPRALVRTYLYGIALKLLAAERRKQARGGPAAESAAEPSA
                     QGAPDVVVWVRQALQKLDAMEREIVMLREYEQLSYAEIAELLRIPVNTVRSRLFRARL
                     ALKNHLQAEPIQPLRARSLGGTRNDEVRDDH"
     gene            985..2310
                     /locus_tag="VEJ00_14145"
     CDS             985..2310
                     /locus_tag="VEJ00_14145"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HXX02352.1"
                     /translation="MTTEHLAEHPIQRDELMAYLDGELPPDRARAAVAHLERCPECQA
                     FAAELQDVSRQLLVWEIEALDAQMPTKIVAALDEREPKANQSKVAAPIREKGILTRRW
                     AWAGAFAAVCVLVGVGVIVPGLHRARQREAVSTSLVEPSGVRGGGGGGGYAAQTRKDS
                     NGLFHGLGDDAANSISHDGQPLIDQDSELAGKSRKYKAWLEPSDSAPQTSGPMIVRTA
                     GITITTKDFDAARAALDQILKRHHGYVGEMNVSTPTGSGRSVTATLRVPADQLDAAMS
                     DLRKLGRIESESQSGQEVTAQYVDLQARLANARNTEQRLTDLLRQRTGKLSDVLAVET
                     EIDRVRGVIEQMEAERKNLAGQVNFATINATVGEDYKAELQVVPVTTSSRIRNAAVDG
                     YRRMVDGVIGVVLFLFSYGPSLLLWGAILFFPARLAWRRVRQLGGKVFD"
     gene            complement(2313..>2844)
                     /locus_tag="VEJ00_14150"
     CDS             complement(2313..>2844)
                     /locus_tag="VEJ00_14150"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HXX02353.1"
                     /translation="NFQRKNSSQPDCDQLRLASGDQRGAIMPLPQTEVPGRLQAHFAE
                     RGIRMTQQRRAILSVMETASKHLDASQILRKAQRLDPSVDRSTVYRTLRLLKRQGLID
                     ELDLMHLNGEGHYYERKLGRDHIHMACLRCGKIMEFVSDEFDKLKQQVEKDCRFRVLV
                     SRLEIGGYCAECRRSQ"
BASE COUNT          537 a          907 c          864 g          536 t
ORIGIN      
        1 cgccgacggt tccaagctca ccgggacatc ctcagcctgg agttccccga ccccctgcac
       61 ggccgaatca ccacctcgtc ctccgaagtc tggagcacca gcgacgacgg cgtcacctgg
      121 cagaagcagt agggcaggct ggaagtgcgc gaaaaactgc ccctgcaggt ggcgttcaga
      181 tgtccgcaac ccgttaccaa agtgacgttt acacaaaatt aggggcctct taccatccat
      241 ctcgtggttt ggcagttctg ggggagggct gccgaggcgt cctggactga aaagtcgagg
      301 gcgcttttct ttttgccccg cgctcgaccg cttttttctc gccgccaggg aacctttctc
      361 ctccccgccg cattacagag gcatgaaaga aattcgacgt ctggcccgga atcgcgacca
      421 tgaccagcga tgaagccctg atgctggagt tccaggcggg atcgcggcag gcgttcgaag
      481 aactcttcgc ccgctaccac gagccgcttt ccggcttctt ccgccgtcgg ctcagcaatg
      541 gcgagcgagc cgaggacctc gcgcaggaga cctttctggc cgtgattcgg gccacgtcgc
      601 gttacgagcc gcgcgccctg gttcgaacct acctgtacgg catcgcgctt aagttgctgg
      661 ccgctgagcg ccgcaaacag gctcggggtg ggccggccgc ggaatccgcc gccgagcctt
      721 ccgcccaagg cgcccccgac gtcgtcgtct gggtccgcca ggcattgcag aaactcgatg
      781 ccatggagcg cgaaatcgtc atgctgcgcg agtacgaaca gttgagctac gccgaaatcg
      841 ccgagttgct ccggatcccc gtgaacacgg tgcgctccag gctatttcgc gcacgcttgg
      901 ctctgaaaaa tcacctgcag gccgagccca tccagccctt gcgagcgaga tccctgggcg
      961 gtacgagaaa cgacgaggtg cgcgatgacc actgaacatc tcgctgaaca tcccattcaa
     1021 cgcgacgaac tcatggcgta tcttgacggc gagttgcccc cggatcgggc cagagcagcg
     1081 gtggcccatc tggagcgttg tcccgagtgc caggccttcg ccgccgagtt gcaggacgtt
     1141 tctcgtcaac tgctggtctg ggagattgaa gcgctggatg cacaaatgcc gacgaagatc
     1201 gttgccgcac tcgacgagcg cgaaccgaag gctaaccagt caaaggttgc tgctcccatt
     1261 cgcgaaaaag gaatcctgac gcgccgctgg gcctgggccg gagctttcgc ggccgtctgt
     1321 gtgctcgtgg gcgtcggagt aatcgttcca ggcctacacc gggccagaca gcgcgaagca
     1381 gtttcgacaa gtctcgtcga acccagcggt gtccgcggcg gcggcggcgg tggtggctat
     1441 gctgcgcaga cgaggaagga ctcaaacggc ctcttccacg gactgggcga cgacgccgca
     1501 aactccatct cccatgacgg ccaaccattg attgatcaag acagcgagtt ggccgggaaa
     1561 tcgcgaaaat acaaggcatg gctggagcct tcggactcgg cacctcagac ttccggtccc
     1621 atgatcgtgc gcaccgccgg catcacgatt acgaccaagg atttcgacgc cgcccgtgcg
     1681 gccctcgacc agattctgaa gcgccaccat ggctacgtcg gcgagatgaa cgttagcact
     1741 cccaccggct ccggccgtag cgtcaccgcg accttgcgcg tgcccgctga tcagctcgat
     1801 gccgccatgt ctgacttgcg gaagctcggc cggattgaat ccgaatcgca gagcggacag
     1861 gaagtcacgg cacagtacgt cgacttgcag gcgcggctcg ccaacgcccg caacactgaa
     1921 cagcggctga cggatctgct ccgccagcgc accggcaaac tttccgacgt gctcgcggtc
     1981 gagaccgaga tcgaccgcgt tcgcggtgtg atcgaacaga tggaggcgga acgcaagaac
     2041 ctggccggcc aggtcaactt cgcgaccatc aacgcgaccg tcggcgagga ctacaaagcc
     2101 gagctgcaag tcgtgccggt taccacatca agccgcattc ggaacgctgc cgtcgacggc
     2161 taccgcagga tggtcgatgg tgtaatcggc gtcgtgctct tcctgttctc ctacggaccg
     2221 agcttgctac tgtggggagc gatcctgttc tttccagcgc ggttggcgtg gcgcagggtt
     2281 cggcagctgg gcgggaaagt ctttgactga tcctattgcg atcgcctgca ctccgcgcaa
     2341 tagccgccga tctccaaccg cgaaaccagc acccggaagc ggcagtcctt ctctacctgc
     2401 tgtttgagct tgtcgaattc gtcactcacg aattccatga ttttgccgca gcgcagacaa
     2461 gccatgtgga tatggtcgcg ccccagtttg cgctcgtagt agtgtccttc gccgttcagg
     2521 tgcatcaggt cgagttcgtc gatcagtccc tggcgcttca acaggcgcaa cgtgcggtag
     2581 accgtgctgc ggtcaacgct cgggtcgagc cgctgtgctt tgcgcagaat ctggctggca
     2641 tcgagatgct tgctcgccgt ctccatcacg ctcaggatgg cgcgccgctg ctgggtcatg
     2701 cggatgccgc gctcggcaaa gtgcgcctgc agccggcccg gaacttcagt ttggggaagc
     2761 ggcatgatgg ctcctcgttg gtccccactg gctagtcgca actggtcgca atccggctgc
     2821 gacgaattct ttcgctggaa gttg
//
LOCUS       DASPUL010000614         8136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_528709, whole genome shotgun sequence.
ACCESSION   DASPUL010000614 DASPUL010000000
VERSION     DASPUL010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528709"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VEJ00_14155"
     CDS             complement(<1..876)
                     /locus_tag="VEJ00_14155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXX02354.1"
                     /translation="MEDVAQPMMRLFINSLAASAGGGLTYIRNVIPHLAANPDVHAII
                     ALGPGLRKEFDEFTNIDFVELETSLSRRFWYEQRALPGLVRRWGADVLLSAGNFAIRN
                     SPVPQILLSRNSIYTSADFNGDLLARNEYRMWLGTRLSGRLAKVSIRWADATVAPSES
                     FAADLRRWSGAAVLAIHHGFDGGAFFCDSAPLDPQVEQELHKAEGCLRLLFVSHYNYY
                     RNFETLFCALPLLRRRLKDRTLRLLITCKLAPGANPGSYDPARAARLVKSLGVSDLVV
                     ELGAVPYRQLHHLYRR"
     gene            complement(918..1922)
                     /locus_tag="VEJ00_14160"
     CDS             complement(918..1922)
                     /locus_tag="VEJ00_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXX02355.1"
                     /translation="MRATNPQAEDQLPARMLDVLACPRDRQPLRQEADDLVCDLGHRY
                     AVIEGIPILLVSDAEQTHIEGTRALLVAQTKDLSSLRQINVGPNEIDPFVMNVIGATN
                     GSLYQHLVGKLTEYPIPRLRLPSGGNKLFLEVGCNWGRWCLAAATAGYRPVGVDPSLK
                     AIRAANRVARQLGVEATYVVGDSRFLPFRDEIFDQVFSYSVLQHLSKADARDSLVQIR
                     RVLQAGGSALIQMPNVFGMRCLYHQIRRRFREPRDFEVRYWRPKELLSTFTNLIGPSE
                     ISADGYFSLNVQPSDLRFLLTRYKMLVYTSEGLRKLSQYVPPLVKAADSLYVSASRIG
                     "
     gene            complement(1996..3417)
                     /locus_tag="VEJ00_14165"
     CDS             complement(1996..3417)
                     /locus_tag="VEJ00_14165"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXX02356.1"
                     /translation="MTERNPRIVVIAAAMVALLALAYFAYTRPGYFTQTTYVGAFLVV
                     EALIAAVWLYRRVFFPLLLVSFLFAGVDLPVGGGWNVARWVFLGTGALVGCFIMLKER
                     NHRFGMFHVLAVFAVLAALVSAAVSLYPDIATLKAASLFLLFVYGSTGARLAVMAREN
                     RFFEGLLIGCEGFVGVLGILYSVGIEMMGNPNSLGAVTSVAAPILLWGVLLEENSFVR
                     RRRWVLYGLCMYLAFHSHARAGMAAAVVSCGLLCFALRRYKLLFQGAAILLILAAASA
                     ILDPPRFSDTVSRLAASVVYKEKDPALGVLSSRISPWQAAVDTLRTHFWFGTGFGTSD
                     TGLDASEHLGKFSSSAKATTENGSSYLAIVAWVGMLGVVPFAMMLAMLVVKIVRTLLW
                     MRRSGNPAHPAIPLAMVLLAGMVHATFEDWMFAVGYHLCVFFWSLAFVFVDLAPSRPL
                     PEFAFALPPGLTPHNVAGVSPSL"
     gene            complement(3425..4561)
                     /locus_tag="VEJ00_14170"
     CDS             complement(3425..4561)
                     /locus_tag="VEJ00_14170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXX02357.1"
                     /translation="MKRRLVILTEIIAPYRIPLFNSLAEEPEIDLRVVFLAETDPELR
                     QWQVHKDGIRFSYQVLPSWRRRFGRYKTLINRGLTRGLAAAAPDVILCGGYSYLASWQ
                     ALFWARLHNVPFLLWSESNRQDQRRSHALVEFLKTEFLSRCSGFVVPGQSALEYLRSH
                     KIREEQIFTAPNAVDNELFSASAAAARQNASRLRKELDLPDRYFLFVGRLVPEKGVFD
                     LLSAYAKLDRSVREQVGLVFVGDGASRSRLAEQSAAISPGVIRFAGFAQQVQVATYYA
                     LAEVAILPTYTDTWGLVVNEAMACGLPVIISRVAGCAADLVAENLNGLLIEPGDVGAL
                     ATAMNTIAGCSELGLTMGSNSVRRISQYSARNWAAGVVRAVHTM"
     gene            complement(4558..5625)
                     /locus_tag="VEJ00_14175"
     CDS             complement(4558..5625)
                     /locus_tag="VEJ00_14175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXX02358.1"
                     /translation="MLGKSGVSVTALAVQPLYRPGLRALESGIPAQWLRYFSLPAGFG
                     LPTAGAFLFARIVGRVRKLHRAQHIDLVHAHAPLPCGHAAMLLSAELALPYVVSVHGL
                     DAFSTAQVHGRAGEWCRRISRRVYASSRRVICISELVREQVLEGVGRSCRTSVVYNGV
                     DPEVFSPGNDSKCDAAPLILSVGNLIPIKGHDLLIRAVAALTPEFPTLKLEIIGNGPE
                     HSRLQALARQLGVAERVRFLGRQSRREVASAMRRCTLFVLPSRYEGLGCVYLEAMSAG
                     KAVIGCRGQGIGEIIRHGSNGFLVGPDNERELTLAMAMLLRDETLRRNLGARGRDTVL
                     DRLTLAQQAESLARIYRECAP"
     gene            complement(5778..7646)
                     /gene="asnB"
                     /locus_tag="VEJ00_14180"
     CDS             complement(5778..7646)
                     /gene="asnB"
                     /locus_tag="VEJ00_14180"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXX02359.1"
                     /translation="MCGIYGIFDPTTRIDDSMRAWSKRAEELLRHRGPDDRGCEERMQ
                     GHCLLGHTRLSIIDLEGGKQPLRNEDDSIWVICNGEIYNYVELRAQLIEKGHRFRTHS
                     DSEVLVHLYEEKGPALLDDLVGMFAFVLLDEKNRRLLAARDRFGEKPLYWVQTGEYGI
                     AFASEMKALTPLPQLDRQLDVAALAQFLALRYIPAPRTHLRSVRKLTAGEALMVSSDG
                     IRRWRYWQPEFPRNGAGKLPAKAEAAEQIRHRIRECVRIRLRSDVPLAAFLSGGIDST
                     FVVSSMRELMPGANFSTFCASFEDEELDESPYARMIAERIGSDHREVHFTTEQILTSF
                     EALIDHYDEPFADASMFPTFAVCRAAHEHCKVMLSGDGGDECFAGYREFFRYYPLHGL
                     RRFPGINAAAGGLVPHWHSSWRGIGLLRFLSSSDWSLLYPDDQRNSLPQIFLAPFAAD
                     AAQGIEELHTRAQHHARLVYPRSAIESMTAGYLPEQILVKVDRASMKSALECRTPFLD
                     RELMEFVRTLPLDYHFERGLGKALLRRALPDWVPEQIRWREKRGFTPPLAAWLRSNLR
                     PQMEEALRDFPAALREILDPAPAWRLFQEHQAGADRSDQLFRWLVLSRRCREAQPA"
     gene            complement(7648..>8136)
                     /locus_tag="VEJ00_14185"
     CDS             complement(7648..>8136)
                     /locus_tag="VEJ00_14185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXX02360.1"
                     /translation="RVPFVVEQAARWPHVQFRIAGIGEEEQLCKSLAAERKCGNVEFL
                     GHLPLSRLGDEMRRADIFLFPSIIEGHPQVLGQAAASGLPAIALKVYRPDYLVDGTTG
                     FLANSDAELSEKLDLLIRQPELRRRMGEAAAAHARKFDWDLIASQWQEAFEQAVARRR
                     KH"
BASE COUNT         1852 a         2441 c         2349 g         1494 t
ORIGIN      
        1 gcgccgataa agatgatgga gttgacgata tgggaccgct cccagctcaa ccacgaggtc
       61 cgatacaccc aacgatttca ccagccgtgc cgcgcgggct ggatcgtagg agcctggatt
      121 cgcgccggga gccagcttgc aagtaattag aagcctcagg gtccggtcct tcaaacgccg
      181 gcgcagcagc ggcagtgcgc agaacagagt ttcgaagttc cggtagtagt tgtagtgact
      241 cacaaacaga agcctgagac atccctccgc cttatgcaac tcctgctcga cttgcgggtc
      301 cagtggtgca gaatcgcaga agaatgcccc gccgtcgaat ccgtgatgaa tggctagcac
      361 cgccgcgcct gaccagcgcc gcaagtccgc agcaaacgat tcactcggcg caacagttgc
      421 gtcagcccag cggatcgaca cctttgccag gcgaccgctc agccgagtgc ccaaccacat
      481 ccggtattcg ttgcgagcaa gcaagtcgcc gttgaaatca gctgaagtgt aaatggaatt
      541 gcgagagagc agaatctgag gtaccggaga gttccgaatc gcaaaattcc cggccgacag
      601 cagaacatcc gctccccatc tccggactag accaggcaac gcgcgctgct cgtaccagaa
      661 gcggcgggac agcgaagtct ccagttctac gaagtcgata ttggtgaact cgtcgaactc
      721 cttccgaaga cctggaccaa gcgcaatgat tgcgtgcaca tccgggttgg cagccagatg
      781 cgggattacg ttacgaatgt aggtgaggcc tccacctgcg ctggcagcca gggagttgat
      841 gaacaaacgc atcatgggct gggctacgtc ctccacggtc tcaatgattg tcgccgtttc
      901 atcgatgaat taccggacta tccaatccgg cttgcagaga cgtaaaggct gtccgcagcc
      961 ttaaccagcg gagggacata ttgacttagt tttcgcagac cttcggaggt gtaaaccaac
     1021 attttgtatc ttgttagcaa aaagcgtaag tcgctgggct gtacgttcag cgagaaataa
     1081 ccatccgcgg aaatctctga cggcccgatt aggttcgtga acgtcgacaa caactcctta
     1141 ggtctccaat agcgcacctc aaaatctctg ggttcgcgaa accgccggcg aatctggtga
     1201 tacaggcatc gcattccaaa aacattgggc atttgaatga gcgcgctccc gcctgcttgc
     1261 aagacccgac gaatctgcac caggctgtct cgcgcatccg cctttgaaag atgctgcaga
     1321 acactgtatg aaaaaacctg atcaaagatc tcgtcacgaa acggcaggaa acggctgtca
     1381 cccacaacgt aggtagcttc cactcccagt tgccgagcga cccgattggc agcccgaatt
     1441 gccttcagcg atgggtcgac gcctactggt cgatatccgg cggtcgcagc agccagacac
     1501 cagcgtcccc aattgcagcc aacttccagg aatagtttgt tgcccccgga tgggagccgc
     1561 aggcgcggga tcgggtattc ggtgagtttt ccaactagat gctggtagag gctcccattt
     1621 gtcgcgccaa tcacattcat aacgaatgga tcaatctcgt tggggcctac gttaatctgg
     1681 cgcaatgatg agaggtcttt ggtctgcgcc accaacaacg cccgggtgcc ttcaatgtgt
     1741 gtttgttccg cgtccgacac aagaaggatg ggaatcccct caatgacggc gtatcgatga
     1801 cccaagtcac aaaccaaatc gtctgcctct tgccggagcg gctgtcgatc tcggggacag
     1861 gccaagacgt cgagcattct tgccggcagc tgatcttcag cttggggatt cgttgctctc
     1921 acttgaccgt actctcgttg ggcctcgact ctctcgcaat accgaggcag caagggcctt
     1981 ggcaaaaccg aagcttcata gactgggact aacgccggct acattgtgcg gcgttagccc
     2041 cggaggcagg gcgaaggcaa actcaggaag tggccgcgat ggggccagat ccacaaaaac
     2101 gaaagcgagg ctccagaaga aaacgcagag gtgatagccg acagcaaaca tccagtcctc
     2161 gaaggtcgcg tgtaccattc cggccagcag aaccatggca aggggaatgg ctgggtgagc
     2221 cggatttccg ctccgccgca tccaaagcaa ggtgcggaca attttcacca ccagcatagc
     2281 gagcatcatg gcgaatggaa cgacacccag cataccaacc cacgccacga tcgccagata
     2341 gctgctgccg ttctctgtgg ttgccttagc agaggaggag aactttccca agtgctcgct
     2401 ggcatccagg ccagtgtcgg aggtgccgaa cccggtgccg aaccagaaat gggtacggag
     2461 ggtatcaact gccgcctgcc acggcgatat tcgcgacgaa agcacgccca aggcagggtc
     2521 tttttccttg tacacaaccg aagccgctaa cctggaaaca gtgtcagaaa acctgggggg
     2581 atcgagaatc gcagaggctg ccgcaaggat gagaaggatg gccgctccct ggaagagcag
     2641 tttgtacctg cgcagggcga agcacaacaa cccgcacgag acaacggctg ccgccattcc
     2701 tgcgcgggca tgactgtgga atgccaggta catgcagagc ccgtacaata cccagcgccg
     2761 ccgccgaacg aaggagttct cctcgagcag tacaccccat aacaggatcg gtgctgccac
     2821 cgaggtcacc gcgcccaaag agttggggtt tcccatcatc tcgatgccga cggagtaaag
     2881 aatcccgagt acgcctacga atccttcgca cccgatgagc aagccctcaa aaaaccggtt
     2941 ctcacgcgcc ataactgcga gtctggcccc ggtgctgccg taaacgaaga gcaagaacaa
     3001 gctggcggcc ttcaacgtgg ctatatccgg gtagagggaa acagctgccg ataccagtgc
     3061 cgccagaacc gcaaacaccg ccagaacatg aaacatcccg aagcggtggt tgcgctcctt
     3121 gagcatgatg aaacagccga ccaaggcccc tgtaccgaga aagacccacc gcgccacatt
     3181 ccagccgccc ccgactggca ggtctacgcc ggcgaacaag aaggacacca acaacagcgg
     3241 gaagaaaacc cggcggtata gccagacggc agcgatcaat gcttccacaa cgaggaaagc
     3301 cccaacgtac gtagtttgcg tgaaatagcc cggacgggtg tacgcgaaat aggcgagggc
     3361 gagcaaagcc accatcgctg cggcgatgac tacgattctg ggattgcgct cagtcacgag
     3421 ggtctcacat cgtgtggaca gcgcggacga ctcctgccgc ccaatttctc gccgaatatt
     3481 gggaaatgcg tcgtacgctg ttgctcccca tcgtcaggcc tagctcggag catcctgcga
     3541 tagtattcat agcagtcgcg agtgccccca cgtcacccgg ttcgatcagc aagccgttca
     3601 agttctctgc aaccaggtcg gccgcgcacc ccgccactcg actgataatt acgggcaggc
     3661 cgcaagccat ggcctcgttc acgactaacc cccaggtatc tgtataagtc ggcaaaatcg
     3721 ccacttcggc cagcgcatag tacgtggcca cctggacctg ctgggcaaac ccagcgaatc
     3781 ttatcactcc aggcgagatg gcagcggatt gttctgccaa ccggctacgg gatgcgccgt
     3841 cgccgacgaa gaccaaccct acctgctctc gtacggaacg atccagtttg gcataagccg
     3901 agagtaaatc aaacactccc ttttccggta cgaggcggcc aacaaaaaga aaataacggt
     3961 caggaaggtc cagctccttt cgcagtcgcg acgcgttttg ccgagccgct gcagctgaag
     4021 cggagaaaag ttcgttgtcg acggcattcg gtgcagtgaa aatttgttct tccctgatct
     4081 tgtgggaacg aaggtattcg agagcggatt gaccgggcac tacgaatccg ctgcaacggc
     4141 taaggaactc ggtcttcagg aactcgacca gagcgtggct acggcgctgg tcctgccggt
     4201 tgctctccga ccaaagaaga aagggaacgt tgtgcaaccg tgcccagaag agcgcctgcc
     4261 aggacgcaag gtagctgtac ccgccgcaca ggatcacgtc gggagcggct gccgccagcc
     4321 cccgcgtcag accgcggtta ataagagtct tatagcggcc gaatcgtctt ctccacgagg
     4381 gcaagacctg gtaggagaat ctgattccgt ccttatgaac ctgccactgg cgcaactcgg
     4441 ggtcagtctc agccagaaaa acgactcgca ggtcaatctc cggctcctca gcaagagagt
     4501 tgaacagcgg aatgcggtaa ggggcaatga tctcagtgag gatcaccaga cggcgcttca
     4561 tggcgcgcac tcccggtaga ttctcgctag gctttcggct tgttgggcca gtgttagacg
     4621 atccaagacg gtgtcgcggc cgcgtgcgcc caaattccgc cggagcgttt cgtcgcgaag
     4681 gagcatggcc atggccagcg tcagttccct ctcgttgtca ggtcctacca gaaaaccgtt
     4741 cgagccgtgc cgaatgattt caccgattcc ctgaccccgg caaccgatga ctgccttccc
     4801 ggccgacatc gcttccagat acacgcagcc gagaccctcg tagcggctgg gcaagacgaa
     4861 tagagtgcaa cggcgcatcg ccgacgcaac ctcgcggcgc gattgacgtc cgagaaagcg
     4921 cacgcgttcg gcaacgccaa gttgccgcgc cagagcctgc agtcgggaat gctcaggccc
     4981 attgccgatt atttccagct tcagcgtggg aaactccggg gtcagcgcgg ctaccgcacg
     5041 aatcagcaaa tcgtggcctt taatcggaat cagattgccg acactcagga tgaggggagc
     5101 agcatcgcat tttgagtcgt tccccggcga aaacacctcc ggatctactc cgttgtacac
     5161 gaccgaggtg cggcagcttc gtccgacgcc ttcgagcact tgctcgcgga ccagttcgct
     5221 gatgcaaatc actcggcgcg aagatgcgta aacacgccgg gagatgcggc ggcaccattc
     5281 gccggcccgg ccgtgcacct gcgcagtgga aaatgcgtcc aggccgtgaa cggagacaac
     5341 atacggcagc gccagttcgg cgcttaagag catggctgca tgcccgcaag gcaagggcgc
     5401 gtgcgcgtga accaaatcaa tgtgctgcgc gcggtgcagt ttgcgcaccc tccctacgat
     5461 gcgggcaaac aggaatgcac cagcggtggg gagtccgaag cctgctggca aggaaaaata
     5521 ccgaagccat tgcgcgggga tgcctgactc caatgcgcga agccccggtc ggtaaagcgg
     5581 ctgcacagcc aacgccgtga ctgagacgcc gctcttgcca agcaaggtga gaggctcata
     5641 tacgaaacag ccaccagtat cgtcgttcac ggaggggtaa aacggcgtaa gcgtcaggac
     5701 atgaataggc cgcgcagact gtgccctggg tgcgactgat cctgggccag ggacaggcgg
     5761 cagattccga gcttcgctca agcgggctga gcctcccggc agcgccggct cagcaccagc
     5821 caacggaaga gttgatcgct gcggtcggca cctgcctggt gctcctgaaa cagccgccat
     5881 gccggcgccg ggtcgaggat ctcgcgcagg gcagccggga aatcgcgcag agcctcctcc
     5941 atctgcggcc gcagattgct tcgcagccac gcggccagcg gcggtgtgaa cccgcgcttt
     6001 tcccgccagc gaatctgctc cggcacccag tcgggcaaag cacgccgcag cagcgctttt
     6061 cccaggccgc gttcaaagtg atagtccagc ggcagagtgc ggacgaattc catcagctca
     6121 cgatccagaa atggggttcg gcattccagc gcagatttca tgctggctcg gtcgaccttc
     6181 accagtatct gctcgggaag atagcccgca gtcatggatt cgattgcaga tctaggataa
     6241 acaagtcgtg cgtgatgctg ggctctggta tggagttcct ctatcccttg ggcggcatcc
     6301 gcggcaaaag gggcgaggaa tatctgcggg agactgtttc gctgatcgtc cggatagagc
     6361 aggctccaat ccgaactgga caggaacctc aacagtccga tgccacgcca cgagctgtgc
     6421 cagtgcggta ccagtcctcc tgctgcagca ttgattcccg gaaaccgtcg caaaccgtgc
     6481 agcggatagt aacgaaagaa ctcgcgataa cctgcgaagc actcgtcgcc cccatcgccg
     6541 gacaacatga ccttgcagtg ctcgtgcgcg gcacggcaga cggcaaaggt ggggaacatc
     6601 gaagcgtccg cgaaaggctc gtcgtagtga tcgatgaggg cctcgaagga agtcaggatc
     6661 tgctcggtgg tgaaatgaac ttcgcggtga tcagacccga tccgttccgc gatcatgcgg
     6721 gcgtagggag actcgtccag ttcttcgtcc tcgaaggatg cgcagaacgt gctgaagttt
     6781 gctccgggca tcagttcgcg catggaactg acgacgaacg tggagtcgat gcccccagag
     6841 aggaaagcgg ccagcggcac gtcgctgcgc agcctgatcc gcacgcattc gcgtatacgg
     6901 tgccgaatct gctccgccgc ttcggctttt gcgggaagtt tgcccgcgcc attccgtggg
     6961 aactcgggct gccagtagcg ccagcggcgg attccgtcgc tcgaaaccat cagcgcctcc
     7021 cccgcggtca gcttgcgaac gcttcgcaga tgggtgcgcg gcgccgggat gtagcgcaga
     7081 gcaagaaact gtgcgagtgc ggcaacgtca agttgacgat ccagctgagg cagcggcgtt
     7141 agcgctttca tttccgaagc gaacgcgatg ccgtactctc cggtctgcac ccaataaagc
     7201 ggcttttctc caaagcgatc gcgcgcggca aggagccgcc tgtttttttc atccaggaga
     7261 acgaacgcaa acatccccac aagatcgtca agcagggccg gacctttttc ctcgtacaga
     7321 tggacgagca cctcggaatc gctgtgggtc cggaagcgat gtcctttttc gatgagttgg
     7381 gctcgcagtt caacgtagtt gtagatttcg ccgttgcaga tcacccaaat gctgtcatct
     7441 tcattgcgca gcggctgctt tccgccttcg aggtcgatga tcgacaggcg ggtgtggccc
     7501 aacaggcagt gcccttgcat gcgctcttcg cagccccggt cgtccggtcc gcggtgccgc
     7561 agcaattcct cggcgcgctt tgaccaggcc ctcatggagt cgtcaatccg tgtcgtggga
     7621 tcgaatatgc cgtagattcc gcacatgtca gtgtttgcgc cgccgggcca cggcctgttc
     7681 gaaagcctct tgccactgag acgcgatcag gtcccagtcg aatttccgcg cgtgcgctgc
     7741 cgcagcctct cccattctcc gccgcaattc aggctgacga atcaacaagt ctaacttctc
     7801 tgaaagttcc gcgtcggagt tggcaagaaa gccggttgtg ccgtctacaa gataatccgg
     7861 gcgatagacc ttcagggcaa tggcgggaag cccgcttgca gcggcttgcc ccaacacctg
     7921 gggatgccct tcgatgatgc tcggaaacaa gaaaatatcg gcacggcgca tttcatcacc
     7981 gagtcgcgag agcggcagat gacccagaaa ctccacattc ccgcacttac gttctgcggc
     8041 gagactcttg cacagctgtt cctcttcgcc gattccggcg atgcgaaact gcacgtgcgg
     8101 ccagcgcgcg gcctgctcaa ctacaaaagg cacgcg
//
LOCUS       DASPUL010000615         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1893432, whole genome shotgun sequence.
ACCESSION   DASPUL010000615 DASPUL010000000
VERSION     DASPUL010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1893432"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /gene="pyk"
                     /locus_tag="VEJ00_14190"
     CDS             <1..1098
                     /gene="pyk"
                     /locus_tag="VEJ00_14190"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HXX02361.1"
                     /translation="GLIELRVRSIRGKDVLCNVINGGLLGEHKGINLPGVALTIPALT
                     EKDRKDLEFGLHHGVDAVALSFVRSAADVRMVKQIIAAHHTRQDGDVPVIAKLEKPQA
                     IDHLEEILEAADGVMVARGDLGVELPPEKVPVIQKQVIRRASAWRKPVITATQMLESM
                     VENPRPTRAEASDVANAIFDGTDAVMLSAETASGQYPREAVAIMARIVVEAEGNMADF
                     EHARRRRDHRRLSVAETICESIAHAAEDLPMGAIAVFTETGNTARMISKYRPQAAIYA
                     FTHVEPVVHRLNLYWGVHPVRCRQARSAEQMVSMAEQDLLRRGQLKPADVLGVVAGTR
                     QASGSTNLMRLHVVTAEEAKRIARSKKKRWQ"
     gene            1336..1632
                     /locus_tag="VEJ00_14195"
     CDS             1336..1632
                     /locus_tag="VEJ00_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02362.1"
                     /translation="MPQEILSVAIWEPVAGQEAASLVTLGELRSILTAKGYGRDLLYR
                     DRESHYVLLRYWSSEEARRAAQEDPDMLRCWARLGNEIQIVKVYETLAPTGDAR"
     gene            1687..2052
                     /locus_tag="VEJ00_14200"
     CDS             1687..2052
                     /locus_tag="VEJ00_14200"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HXX02363.1"
                     /translation="MIENLYDLIGGQQTIKAATERFYEKVLQDERLRGFFAQADMAHL
                     RSRQVMFVSMLLGGRVYTGKDIRDAHALARNQGMTHEHFDLFLKHFRDALEEVGVEPE
                     NAAKVIQPLESKRKVVLDT"
     gene            2084..>2399
                     /locus_tag="VEJ00_14205"
     CDS             2084..>2399
                     /locus_tag="VEJ00_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02364.1"
                     /translation="MSKTSIRQFLDESVDPKVRGYLHSPEASNGNALILTHGAGSNAQ
                     APLLVALAETFCAAGFTVLRCDLPYRQVRSYGPPGPGDAARDRAGLKNAVAALRTIVL
                     GAP"
BASE COUNT          482 a          834 c          684 g          399 t
ORIGIN      
        1 ggcctgatcg aactgcgcgt gcgcagtatc cggggcaagg acgttctctg caacgtcatc
       61 aacggaggcc tgctgggcga gcacaaaggc atcaaccttc ccggggtcgc gctcaccatt
      121 ccggctctta cggaaaaaga ccgcaaggat ctcgaattcg gcttgcacca cggggtcgac
      181 gccgtcgctc tctctttcgt gcgcagcgca gccgacgtcc gcatggtcaa gcagatcatc
      241 gccgctcacc atacccggca agacggcgac gtgcccgtca tcgcgaaact cgaaaagccg
      301 caagccatcg accatctgga ggaaattctc gaagccgccg acggagtcat ggtcgcccgc
      361 ggcgacctcg gtgtcgagct cccgccagaa aaagtcccgg tcatccagaa acaggtcatt
      421 cgccgcgcct ccgcctggcg caagccggtc atcaccgcca cgcagatgct cgagtccatg
      481 gtcgaaaacc cgcgccccac ccgcgccgag gccagcgacg tagccaacgc catcttcgac
      541 ggcaccgacg ccgtcatgct ctcggccgaa actgccagcg gccagtatcc ccgcgaggcg
      601 gtcgcgatca tggcccgcat cgtggtcgag gccgagggca acatggccga cttcgagcac
      661 gcccgccgcc gccgcgacca ccgccgcctc tccgtcgccg agaccatctg cgaatccatc
      721 gcccacgctg ccgaagatct gcccatgggc gcgatcgccg tattcactga aaccggcaac
      781 accgcccgca tgatttcgaa ataccgcccc caagccgcga tctacgcgtt cacccacgtc
      841 gagccggtcg tccatcgcct gaacctgtac tggggagtcc atcccgtgcg ctgccgccag
      901 gctcgctccg ccgagcagat ggtctcgatg gccgagcagg atctcctgcg ccgcggccag
      961 ctcaagcccg ccgacgtgct gggagtcgtc gccggcacac gccaggcctc gggctcgacc
     1021 aacctgatgc gcctgcacgt cgtcaccgca gaagaagcca agcggattgc ccgctcgaag
     1081 aaaaaacgct ggcagtaatc gtatgtgcag ccatcaacac ccgtgtgcga gtgaaaactg
     1141 gtcccggagg gaccatcgat agtagcccgc cagtttactg gcgggtcacg atccgaggcg
     1201 gtaggaccgt gccggaggca cggcggaaac caaccatggg agcgttcagg cgcccctccg
     1261 gaacattgtg gtcggtaggg tagatctgaa cgccgacgcc gttccatgcc gccccccacg
     1321 ttataatccc ccggtatgcc ccaggagatc ctctcggtgg ccatctggga acccgtcgcc
     1381 gggcaggaag ccgcgtcact cgtcacgctc ggcgaactga gatcgatcct cacggccaag
     1441 ggctacgggc gcgatcttct ctaccgcgac cgcgaatcgc actatgtcct gctgcgttac
     1501 tggagctccg aagaagcccg ccgcgcggcc caggaagatc ccgacatgct ccgctgctgg
     1561 gcgcgccttg gtaacgagat tcagattgtg aaggtctatg aaaccctggc accaaccggc
     1621 gatgcgaggt gactaagagc gggccgccac agctcgcggt ggcaacctca caagcgagaa
     1681 gacgccatga ttgagaactt atatgacctg atcggcggac agcagaccat caaggccgcg
     1741 accgagcgtt tctatgagaa agttctccag gacgaacgac tccgcggctt ctttgcgcag
     1801 gctgacatgg cgcatctccg ctcccggcag gttatgttcg tctccatgct cctcggtgga
     1861 cgggtatata cgggcaagga cattcgcgat gcccacgccc tggccaggaa tcaaggcatg
     1921 acccatgagc acttcgacct ctttctcaaa cactttcgcg atgctctcga agaagtcgga
     1981 gtggagccgg aaaatgccgc gaaagttatc cagcctctgg aaagcaagcg caaggttgtc
     2041 ctcgatacat aagacttccc ccgattcaaa atcggcggca cccatgtcca agacgtccat
     2101 tcggcagttc cttgacgaaa gtgttgatcc aaaggtccgc ggctatctcc actcccccga
     2161 ggcatccaac ggcaacgccc tcatcctcac ccacggcgct ggctccaacg cgcaggcccc
     2221 gctcctcgtc gctctggccg agacgttctg tgcggcagga ttcaccgtgc tgcgctgcga
     2281 tttgccctac cgccaggttc gttcctacgg accgcccggc ccgggcgatg ccgcccgcga
     2341 ccgcgccggc ctgaaaaacg ccgtcgcggc tctcagaact atcgttttgg gtgccccat
//
LOCUS       DASPUL010000616         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1764607, whole genome shotgun sequence.
ACCESSION   DASPUL010000616 DASPUL010000000
VERSION     DASPUL010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764607"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VEJ00_14210"
     CDS             <1..516
                     /locus_tag="VEJ00_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02365.1"
                     /translation="RNRARGCGAGAADKAVGEQRNGSRYEVRYSLILNTGASNGICVS
                     GPCAPQAWAAGRRTKKAVSQVLTGSGAISRDDRKRKKAEYFMENLGESVKYLCSAFEY
                     LRALHGTLEALMVDVAALRQVVLKEDKAARHYRKVLAEAVKEARPLVATAMQAYDTEI
                     ARIKTAGTWKN"
     gene            896..>2138
                     /locus_tag="VEJ00_14215"
     CDS             896..>2138
                     /locus_tag="VEJ00_14215"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXX02366.1"
                     /translation="MAKSAASTVTLHSRLRSTKLHVAEALALAAEAGRALAEAHAVGR
                     IHGNLTSTNILLQDSSGPKVTIESFGGAPETGDAKHLAPERIAGGPPSVAADIYSFGR
                     ILEEIRNSTTFEPATGKQWDAAVLRCLAPAPDQRFPSVEAVLQAIVVEGQTLSVAIAS
                     PAMEGPKRWGQFQLLQQLGMGGFGEVYRAWDLTLEREVALKLLLPRGLKPEEEFASIV
                     SEARAMARVRHPNTVSVYGVDRHDGRVGLWSDFVRGQTLAQWVDTEGPRSEKETVEIG
                     TQLCDALSAVHHAGLLHRDIKPGNAMRAEGGRILLMDFGLSHELHRETRWGGTRDYMA
                     PELYAGSPPSIQSDIYAMGVLLQFVCTGGHQLGNSLPSREAGPQTAISPALRRIIDTA
                     TNVDPRERYTSAAKLHDALATL"
BASE COUNT          449 a          630 c          689 g          370 t
ORIGIN      
        1 cggaaccgcg cgcggggttg cggcgcgggc gcggcggaca aggccgttgg tgaacagcgc
       61 aacggctcta gatacgaagt aagatatagc ttgattttaa ataccggcgc gagtaatggt
      121 atatgtgttt cggggccatg tgctccgcag gcttgggctg ctggccgccg aacaaagaaa
      181 gccgtaagtc aggtcctcac tgggtcagga gccataagtc gcgatgaccg caagaggaag
      241 aaagcggaat actttatgga gaacttgggg gaatctgtaa agtatctgtg ctcagccttt
      301 gaatacctga gagctttaca cggaacactg gaagcactga tggtcgatgt cgccgccctc
      361 cgccaggtcg ttttgaaaga agacaaggcc gcgcggcact atcgaaaagt gctggcagaa
      421 gcagtgaaag aagcacggcc gctggttgcc accgcgatgc aggcctacga tacggaaatc
      481 gcgcgaatca aaaccgcagg tacctggaaa aactgagagg aacaagggcc cgggccggcc
      541 agggttttgg acggcctcgg accgacggcg gccgacgaat ctgttctgct ccgatcggac
      601 gcgccttccg tgtcttgagg cggggctccc cgtcgtcttg ccgacggatt caggtcctaa
      661 cggacgatat caagcgaagg ggtttgggaa ccctgtcgag tgtcgccgat accgaaggcc
      721 ctaacttatt gatttgtgac atgttgcagg ccgttcgccc atggccttgg cgcgtcggat
      781 tcttgccctt tcgacgcgac gcgaccctag gcggcaacca aacacgaatc cgcagtgaag
      841 acggcgggaa cccattataa tctgagccgc caacgagcag cccagggatc gaaccatggc
      901 gaagtcagcc gcctccacag tgacgctgca cagccgcttg cgaagcacga agctgcatgt
      961 ggcggaggcc cttgcccttg ccgcagaagc aggcagagca ctcgccgagg cgcacgccgt
     1021 cgggcgaatc cacggaaacc tcaccagcac aaacatcctg ctgcaggatt cctcagggcc
     1081 caaagtgacg atcgaaagct ttggcggcgc tccagaaacg ggcgacgcca agcatctggc
     1141 tcccgaacgg atcgccgggg gtccgcccag cgtcgccgcc gacatttact ccttcggacg
     1201 aatcctcgag gagatccgca attcaaccac gttcgagcct gccaccggca agcaatggga
     1261 cgcggcggtc ttgcgctgtc tcgctcctgc tcccgatcag cgatttcctt ccgtggaagc
     1321 cgtgctgcag gcgattgtcg ttgaagggca gaccctctcg gttgcgattg ccagccctgc
     1381 catggaaggg ccgaaacggt ggggccagtt tcagctcctg cagcaactgg gcatgggggg
     1441 gtttggtgaa gtctatcgag cctgggatct gacgctggag cgagaagtcg cgctcaaact
     1501 tctgctgccc cgcgggctaa agccggaaga agagttcgct tccattgttt cggaagcacg
     1561 ggcgatggcc cgcgtgcgcc accccaacac ggtttccgtc tacggggtag accgccacga
     1621 cgggcgggta ggattgtgga gcgattttgt ccgtgggcaa acgcttgcgc aatgggtgga
     1681 tacggagggg ccgagatcgg aaaaggagac cgtggagatc gggacgcagc tttgtgacgc
     1741 gctcagcgcc gtccaccacg cgggcttgct gcatcgggac atcaaacccg gcaacgccat
     1801 gcgcgccgaa ggcgggcgca tcctgcttat ggattttggc ctgagccacg agctccaccg
     1861 ggaaacgcgc tggggcggca cgcgcgacta catggcgccc gagctctacg caggcagtcc
     1921 gccctcgatc cagagcgaca tctacgccat gggcgtgctg ttgcagtttg tctgcactgg
     1981 cgggcaccag cttggcaaca gcctcccgtc gcgggaggcg ggaccgcaga cggcaatctc
     2041 gccggcgctg aggcgcatca ttgacaccgc gacaaacgtt gacccgcggg aacggtacac
     2101 cagcgcggcg aagctgcacg atgccctggc gacgcttg
//
LOCUS       DASPUL010000617         3493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1073000, whole genome shotgun sequence.
ACCESSION   DASPUL010000617 DASPUL010000000
VERSION     DASPUL010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073000"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VEJ00_14220"
     CDS             complement(<1..935)
                     /locus_tag="VEJ00_14220"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXX02367.1"
                     /translation="MKRIVVCFLVLLLGCPAFAASKDSPTLVIQNVTIIDVTGGALLS
                     HRTVVVRDGKIADITESGADRGDKKAVHVNGTGKFLIPGLWDMHVHMVFGEWFPHGKE
                     ITVPLFIANGITGVRDMGSELEVLQQWRKEIEAGTLIGPRIVMAGPMLDGPKPRFPSS
                     IAITTPEDGRRAVDDLKRRGVDFIKLQSLIPRDAVFAIADEAHKQGITFVGHVPDSVR
                     ASEMSNAGQKSFEHLIGIFEGSSPLEDEFIKGDKNETKFLATYDPKRAADLFALLAKN
                     QTWQCPTLVWERGGNLIDLSDFAHDPRAKYVPASW"
     gene            complement(1066..1623)
                     /locus_tag="VEJ00_14225"
     CDS             complement(1066..1623)
                     /locus_tag="VEJ00_14225"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HXX02368.1"
                     /translation="MTLTMIPRWQKPAVHSLLAFFLTTAAAAQSGKGPSVTMAPVALV
                     TAPRAAQTMVELNFRVGSGFHINSNTPKSEFLIPTSLKMDLPTDIVLGKIEYPDGQDL
                     SFPFSPDEKLNVYTGDFTVSVAVHPLHSVVPGKYVMHGVLRYQACDNAQCFPPKTLPV
                     SFDVKVVKEPPGPRHNPAQSPHVHN"
     gene            complement(1702..3141)
                     /locus_tag="VEJ00_14230"
     CDS             complement(1702..3141)
                     /locus_tag="VEJ00_14230"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HXX02369.1"
                     /translation="MSNLPLPILAILAFVGGLVSFVSPCVLPLVPGYVSMISGVAVEE
                     LKSAQSRLLRRVMINSIAFIVGFSVVFIGEGAAATGVGQALGIYKHTLARLAGVVIIL
                     FGLHLTGIFRIKALYTDARLHAVKGGSTPVGAFVIGFAFAFGWTPCLGPILATILGFA
                     SQQDTVGKGIMLLSVYSLGLAMPFLLTSLLMEQFLKFYSRFRSYMHAIEVASGGLMIA
                     LGVLLLIGRFTMISNWLSFLGTFEVWLEGVVTKANPATILAGALVVAVAVALALYMVY
                     RRGRSSNERVVMKRNPLVLVVVAVVVAGMLFFGFHAARRSGGATVAAMSSVAPDFTLE
                     SLDGKSVRLSDLRGKAVLLNFWATWCGPCKIEMPWFVELQSRYGPEGLQIVGVAMDDA
                     SKEDIAKFAKDMGVNYPVLIGKEAVGDEYGGVPALPESFFIGRDGKIVDKIFGLKGKG
                     EIEDAIKKALNTQPGTQATPPVTTTQAQN"
     gene            complement(3269..>3493)
                     /locus_tag="VEJ00_14235"
     CDS             complement(3269..>3493)
                     /locus_tag="VEJ00_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02370.1"
                     /translation="FLAMFRSAETSVGSPGSDADLKALFDSLHVRQEGSRAVLYAIVP
                     TAVLRKALESPGPLPPASPAVAPGQPGRAH"
BASE COUNT          731 a         1070 c         1035 g          657 t
ORIGIN      
        1 ttccatgacg cagggacgta cttggcgcgc gggtcgtggg cgaagtccga gagatcgatc
       61 aggttgccgc cgcgctccca caccaaagtc ggacactgcc acgtctgatt ctttgccaga
      121 agcgcaaaaa gatccgcggc acgcttggga tcgtaggtcg ccaggaactt ggtttcgttc
      181 ttgtcgccct tgatgaattc gtcttccagc ggcgagctgc cttcgaagat tccgatcagg
      241 tgctcgaaac ttttctgccc ggcattcgac atctccgagg ctcgcaccga atcgggcacg
      301 tggccgacaa acgtgatgcc ctgcttgtgc gcttcatccg cgatggcgaa aactgcgtcg
      361 cgcggaatca acgactgcag cttgatgaag tccaccccgc ggcgcttcag atcgtcgacc
      421 gcgcgacgtc cgtcctcggg cgtcgtgatc gctatcgagc tgggaaagcg cggcttcggg
      481 ccgtcaagca tggggccggc catgacgatg cgcgggccaa tcagcgtgcc agcttcaatt
      541 tccttgcgcc actgctgcaa cacttccaat tcgctgccca tgtcgcgcac gccggtgatg
      601 ccgttggcga tgaacagcgg caccgtgatc tctttgccgt gcgggaacca ttcgccgaaa
      661 accatgtgca cgtgcatgtc ccaaaggccc gggatgagga acttgccggt gccgttgacg
      721 tgaacggcct tcttgtctcc gcggtcagcg ccagattcgg taatgtcagc gatctttcca
      781 tcgcgtacca cgacagtgcg atgcgacaag agcgcgcctc ccgtcacgtc gatgatggtg
      841 acgttctgga tgacgagagt tggcgagtct ttagacgcgg cgaacgccgg gcatcccaaa
      901 agcagcacga gaaagcagac gacgatgcgc ttcatggatg gaaatcttat ggacgaaggg
      961 cgtgcgagtc aaaagatgaa aaaggtcatg tagggacagc cgccctcggc tgtccgtcga
     1021 gcacaactcg acatgatctg ctccggacga atgcgttctg ggatgctagt tgtgcacgtg
     1081 cggactctgg gccggattgt gacgtgggcc gggaggttcc ttcaccacct tcacatcgaa
     1141 gctcaccggc agcgttttcg gcgggaagca ctgcgcgttg tcgcaggcct ggtatcgcag
     1201 cacgccgtgc attacgtact ttcctggaac taccgagtgc aggggatgca ccgccacgga
     1261 gacggtgaag tctcccgtgt acacattcag cttctcatcc ggagagaatg gaaaactcag
     1321 gtcctgaccg tcaggatatt cgatcttccc caggacgatg tcggtcggca ggtccatttt
     1381 cagtgacgta ggaatgagaa actctgattt cggcgtgttg gagttgatgt gaaaaccgga
     1441 gccgacacgg aaattgagct cgaccatggt ctgtgctgcg cggggcgccg tgaccagagc
     1501 aaccggagcc atcgtgaccg aaggcccttt gcccgattgc gccgccgctg cggtggtcag
     1561 gaagaacgcc aacagcgagt gcacagcggg tttttgccag cgcggaatca ttgtcagggt
     1621 caatagtaga ttgccgggtg ccttgttcct agtgtcactt gtggccctag acgaatgcgt
     1681 ccgggattac agggtcttag gctagttttg agcctgcgtc gtcgtgaccg gcggcgtggc
     1741 ctgggttcca ggctgggtat tcagcgcctt cttgatcgcg tcctcgatct cgcctttgcc
     1801 cttgaggccg aagatcttgt cgacaatctt gccgtcgcgg ccgataaaaa agctctccgg
     1861 cagagctggc acgccgccgt attcatcgcc caccgcttct ttcccgatca gcacggggta
     1921 gttcactccc atatccttgg cgaacttggc gatgtcttcc ttgctggcgt cgtccatggc
     1981 cacgccgaca atctgcaggc cctctgggcc gtatctgctc tgcagttcga caaaccatgg
     2041 catctcgatc ttgcacggac cgcaccaggt cgcccagaag ttcagcagga cggccttgcc
     2101 gcgcagatcc gaaagacgca cgctcttgcc gtcgagcgat tcgagagtaa agtccggcgc
     2161 caccgatgac atcgcagcca cggttgcgcc gccggagcga cgcgccgcgt gaaagccgaa
     2221 aaacagcatt cccgcgacca cgacagcgac cacgaccagg acgagaggat tacgcttcat
     2281 gactaccctt tcattcgaac ttcgcccgcg gcgataaacc atgtacagtg cgagtgccac
     2341 ggcgacggcc accaccagcg ccccggccag aatcgtagcc gggttggcct tcgtcaccac
     2401 accctccagc caaacctcga acgtgcctaa gaacgacagc cagttcgaaa tcatggtgaa
     2461 gcgcccgatc aacagcagca ctcccagcgc gatcatcagg ccgccgctgg ccacttcgat
     2521 ggcgtgcata taggagcgga aacggctgta gaacttgagg aattgttcca tcagcagcga
     2581 cgtcagcaga aacggcatgg ccagccccag cgagtacacc gacagcagca tgatgccttt
     2641 gcccacggtg tcctgctggc tggcgaagcc caggatcgtg gccagaatcg ggcccaggca
     2701 gggcgtccag ccaaaagcga aggcgaagcc aatcacgaac gcgcccaccg gcgtgctgcc
     2761 cccctttacg gcgtgcaggc gagcgtcggt gtagagcgct ttgatcctga aaatgccggt
     2821 cagatgcagc ccgaacaaaa tgatgaccac gcccgccaac cgcgccagcg tgtgcttata
     2881 aattcccagc gcctgaccca ctccggtggc cgctgctcct tctccgatga acaccacgct
     2941 gaagccaacg atgaaggcga tagaattgat catcacacgg cgcaaaagcc gggactgcgc
     3001 cgatttcaac tcttctacgg cgacgcccga aatcatagac acgtatccgg gcacgagggg
     3061 aaggacgcag ggcgacacaa acgaaaccag gccccccacg aacgcgagaa tggccagaat
     3121 agggagtggg aggttcgaca tcgtgtttat tgtacctgct tgactgcctc gtaagaccgg
     3181 gtccctagac ctagtcctac cgaatcaggt gctacagaat aagacgtgcg gcgcggcatt
     3241 tagttgcacg ccgatccggc agccgcggtc aatgcgccct gccgggctgc cccggagcca
     3301 cggcgggact ggcaggcggc aacgggccag gcgattccag cgccttgcgc aacacggcgg
     3361 ttggcacgat cgcatacagc accgcccggc tcccttcctg acggacgtgg aggctgtcga
     3421 acagagcctt caagtcggca tcgctacccg gcgatccgac tgaggtttcg gcgctgcgaa
     3481 acatcgccag aaa
//
LOCUS       DASPUL010000618        12446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1344923, whole genome shotgun sequence.
ACCESSION   DASPUL010000618 DASPUL010000000
VERSION     DASPUL010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 12446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12446)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12446
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344923"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VEJ00_14240"
     CDS             complement(<1..280)
                     /locus_tag="VEJ00_14240"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXX02371.1"
                     /translation="MNEIEFETALKRAGYVDVERKQVPPDHITEPHAHPFDVRALVLA
                     GEVTLTSQGESRTYRAGSVFEMAAGCEHSERHGSEGTKYLVGRRRAAVS"
     gene            complement(318..704)
                     /locus_tag="VEJ00_14245"
     CDS             complement(318..704)
                     /locus_tag="VEJ00_14245"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HXX02372.1"
                     /translation="MTIQIVRLGTTRAPSEGLRIGTVRRPPRGVPKAKFALENWYDVW
                     LPNLAPSEATVKLGRGAATEREWGAFKRRYRAEMAAPDASRVLDLLAALSHTTDFSVG
                     CYCEAEDRCHRSVLRALLLARGAEVK"
     gene            939..1199
                     /locus_tag="VEJ00_14250"
     CDS             939..1199
                     /locus_tag="VEJ00_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02373.1"
                     /translation="MKTTIELPDALFRRAKSKAAESGMTMKQFFTEAIQDKLAGKFPN
                     AAPGQPAWMHGFGKLRRLRKETQKVQQAIDDEFGIVEPEDRL"
     gene            1196..1573
                     /locus_tag="VEJ00_14255"
     CDS             1196..1573
                     /locus_tag="VEJ00_14255"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXX02374.1"
                     /translation="MILDTNALSAFIDGDTVLGEVLSQQQRAAIPVIVLGEFRYGIAG
                     SKYRSSYEQWLDIHLGDFEVLPVTAETATAYASVRVALKRAGTPIPANDAWIAALALR
                     HRLPVLSRDAHFDVVLGLQRVSW"
     gene            2205..2489
                     /locus_tag="VEJ00_14260"
     CDS             2205..2489
                     /locus_tag="VEJ00_14260"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HXX02375.1"
                     /translation="MKRKLTDTKVTAKQRRELKALVARPERRIDTSDIPPLTDAFRKN
                     AQRNPFYRVCKTSTTVRIDADVLAWLKSKGRGYQTRLNHILRDAMLAEVK"
     gene            2848..3090
                     /locus_tag="VEJ00_14265"
     CDS             2848..3090
                     /locus_tag="VEJ00_14265"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HXX02376.1"
                     /translation="MDEFGASEVKNKLAALLDLVEQGKEITITRWGKPVAKLVPAGPG
                     FDRDKARAAAAQLLEASRGVKLGRLKLKDLIAAGRP"
     gene            complement(3549..3992)
                     /locus_tag="VEJ00_14270"
     CDS             complement(3549..3992)
                     /locus_tag="VEJ00_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02377.1"
                     /translation="MSLRDWLRNSWLVEHETSPEEIAGLLAIVERDLANAKVAGLSED
                     WQLNIAYNAALQAATAALAAAGYRATREQHHYRTIQSLAYTVGWPPAAVGRFDRFRKK
                     RNITGYETAGVISMAEAQEMYKLAEGLRDDVVSWLKKNHPKLLKS"
     gene            complement(3964..4617)
                     /locus_tag="VEJ00_14275"
     CDS             complement(3964..4617)
                     /locus_tag="VEJ00_14275"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXX02378.1"
                     /translation="MTRLPTMGILLPMVGSQGITPEGSVSLFGKTRQTLLALLYTRAD
                     QEHLQESLIQLAGLGRGTVQRELEFLARAGVVRRTVRGRQVYFQANPESPIFGELRGL
                     VVKTAGVADALRAVLAPLAGRIRVAFVYGSVAQGTERRGSDLDVMVLGDVSFAEASEA
                     LAGAQGAIGREVNPTVYPPAEFRTKLAAKHHFLRSVLRAKKIFLIGDEHELARLAKK"
     gene            4740..5213
                     /locus_tag="VEJ00_14280"
     CDS             4740..5213
                     /locus_tag="VEJ00_14280"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXX02379.1"
                     /translation="MRSSRIPPPLWLLIFAVAMWALNHYYPIVTIISEPWNRLGWSVI
                     AIAPLAPIAALIQFRRAHTTVNPHRPEMTTTLVTSGVYAWTRNPMYLGLSILLLGWAI
                     KLGTLSPFAGPLLFIPLIQRVQIRPEEHALRMQFGNDFDQYCQRVNRWFGRTSQA"
     gene            complement(5367..6092)
                     /locus_tag="VEJ00_14285"
     CDS             complement(5367..6092)
                     /locus_tag="VEJ00_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02380.1"
                     /translation="MSPPTVSWAKSGDVPPTTQLDISGEFSKRITENLGISIGTGWTQ
                     FSTPGSLRASGFDNLETALQYQFVRDAEHEFALSAAINVEWGGIGASAVGSDSFSTVT
                     PSVLFRKGLGDLPDKMGWARPLALTGQIGYSFPTRTSTNSVDPDTGLPTSMANPQFLT
                     YSTLQYSMPYLKSSVVDLDLPAFFNHLIPIVEAQFTTPTANNSGTGIGTTGTINPGVI
                     WVGSSLRIRSFGRRASSWRDQRA"
     gene            complement(6498..7607)
                     /locus_tag="VEJ00_14290"
     CDS             complement(6498..7607)
                     /locus_tag="VEJ00_14290"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HXX02381.1"
                     /translation="MPDPLPPQGPPWEVDLKRAKLLARAEQAVARLEVAGEMVPSLDW
                     FIYAFVRKEAVVSSQIEGTQATLVDLFEYEALQDHREPLEDVREVCNYLDALKYARAQ
                     LASSKGLPVSMRLLHAAHRRLMRGVRGAGKQPGEVRRSQNWIGGTRPGNAVFVPPPPE
                     EVPALLGDLEKYFHAADSLPPLVRIGLAHVQFETIHPYLDGNGRIGRLLITLLLEHWK
                     VLSAPLLYLSLFFKRHRAEYYRLLGEVREHGDWEAWTDFFLEGVATIADEAVVAARDV
                     FALVTAHRRSLLAASGASVVAARLLEQLPVHPVITIPGIVKLLKITKPTAGKAVQLLE
                     SVGVLVETSGKARDRTFAYQAYLERLRTGTELEGR"
     gene            8477..9061
                     /locus_tag="VEJ00_14295"
     CDS             8477..9061
                     /locus_tag="VEJ00_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02382.1"
                     /translation="MNSLSSGKSLPHRLWKKLILGAMCFSAVLILTPLTWAQEKRPIA
                     VSGEGAKGRYLQQYIIDVDDTPGHQIRINEIQRTYAPDKQAVIEGERIVETWSRSLTN
                     YISGIGPVLVAYNTWVTDKGNKIFIEGTGTSESQITDTGSKRGTYHGTSRFVGGTGRF
                     AKIRGTLVDVSTFDTDPKSGYNTIDSHGQYWFEE"
     gene            9115..9612
                     /locus_tag="VEJ00_14300"
     CDS             9115..9612
                     /locus_tag="VEJ00_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02383.1"
                     /translation="MKRLQTWMSLASVAICLITPVSSRAESGTMDVLISTVSVLTSIP
                     MGDTTVTARSGSGTVTFIKSSGGPFVEGASGAAQFASFSKKSATGFDLEADGLVTFSA
                     EDTLQLLFKRRTGDLAAGTSGEGTLQLTGGTGRFSGISGQCKYKVENLSGNWQVTMAK
                     CQWSR"
     gene            9696..10088
                     /locus_tag="VEJ00_14305"
     CDS             9696..10088
                     /locus_tag="VEJ00_14305"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HXX02384.1"
                     /translation="MTKNAYGLTLQRRILSGVFLVMLSLFALPAFSEEVLRIVSFVAA
                     GPEQQKELQKIAPGKLYTSAKGCRWVKFWSDPATGETGSVSLWKSRADLDAFLNSDAY
                     KAIFPGKVSSLAKGDVSVKVYPVVKPKH"
     gene            10489..10920
                     /locus_tag="VEJ00_14310"
     CDS             10489..10920
                     /locus_tag="VEJ00_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02385.1"
                     /translation="MKAQHASIKITLLLLASCMLSSAFAADAEQTYLVAYAKFKPGKA
                     PEALKIIHEHFWPVDKKVGRKAIPFDFLTGEWDHVVYFPYDTARMDTIPPGAEWWKAL
                     AEQEGGAEQAQRLFQSYLDLEANSKFEVAKSPGPLMSTSSR"
     gene            complement(11157..>12446)
                     /locus_tag="VEJ00_14315"
     CDS             complement(11157..>12446)
                     /locus_tag="VEJ00_14315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXX02386.1"
                     /translation="VNHRVIAQAGSLRAPPVQPDPDQDAITRFWEAGADLLVKPILKL
                     AMSEPNEDFQDIAPGTYRLEGREIDDALAGQTGTRRRLTPDSRAVVLSAAQPVYLEDK
                     VVGAVLVEETTNDVLALRNRAFEKLFAALIVVFTIGTIGLLVFATRLSVRIRRLRDEA
                     EHSIDAHGRVTGTVGGSDAADELGDLSRSFADILQRLQQYTGYLETLGGRLSHELRTP
                     VAVVRSSLDNLKQVALPDEAKIYLHRAEEGLTRLSTIFTRMSEATRLEQSLASVERQC
                     FDLAEVAGGCVEGYRLAHRDRRFAFDAGPGPFPVNGAPDLVAQMLDKLAANAVEFSTK
                     DSTIHFALQRHGSAARLSVTNAGPPLPADMQDRLFESMVSVRKEQDQQDPHLGLGLYI
                     VRLISEFHGAQALARNLEDGSGVEIRVEFPLQENGTG"
BASE COUNT         2507 a         3719 c         3784 g         2436 t
ORIGIN      
        1 agctgacagc cgcacgcctc cgcccgacga ggtactttgt cccctcactg ccgtgccgtt
       61 cgctgtgctc gcagccggcg gccatctcga agaccgagcc ggcccggtag gtgcgggact
      121 cgccttgcga tgtcagtgtg acttcacccg ccaggacgag cgcgcggacg tcgaagggat
      181 gagcgtgcgg ctcagtgatg tggtcaggcg gcacctgctt cctttcaacg tccacgtaac
      241 ccgcccgctt cagcgccgtt tcgaactcga tttcgttcat ggttccccct ttggagcctg
      301 ctgtgagatg ccttcgctca ttttacttcg gcgccgcgcg cgaggagcag tgcgcgcagt
      361 accgaccggt ggcagcggtc ctcggcctcg caatagcagc caaccgagaa gtcggtggtg
      421 tgtgaaagcg cagcgaggag atcgagcacc cggctcgcgt cgggcgccgc catttcagcg
      481 cgatagcgcc gcttgaatgc gccccactcg cgttccgtgg cggcgccccg gccgagcttg
      541 accgtcgcct cggacggagc gaggttgggc agccacacgt cgtaccaatt ctccaatgcg
      601 aacttcgcct tgggcacccc ccgcggcggg cggcgcacag ttccgatgcg cagaccttca
      661 ctgggcgcgc gcgtagtgcc caggcgcacg atctggatgg tcatgggccg gcgctgccac
      721 tcctgaggtt cgtcgccagg tccctcatgt agtcgccttc agccgaagag ttctaggtcg
      781 tcgaagagcc cgatgccggc cagccactgt ttcaccgcca ccttagcatg tactcaatgc
      841 cggcttcgtc gaagcgagcg ataagccacc tctagcattg tatgtcgggc ggtctacccg
      901 cagacaagtg cagaccataa atatggtaat atgggcggat gaaaactacg attgagctcc
      961 ctgatgcact gttccggcgc gccaagtcca aggctgcgga gagtggcatg accatgaaac
     1021 agttctttac cgaagccatt caggataagt tggcgggtaa gtttccaaac gccgctcccg
     1081 ggcaacctgc atggatgcac ggcttcggca agctgcgccg cctgcgcaag gagacgcaga
     1141 aagttcagca agccatcgat gacgaatttg gtatcgtcga gccagaagac cggctgtgat
     1201 cctggatacc aatgcgctat cggcgttcat tgacggcgac accgtgctgg gggaagtcct
     1261 gtcccagcag cagcgtgcgg ccatcccggt gattgttctg ggcgaattca gatacggcat
     1321 cgccggctcg aaataccggt cgagctacga gcaatggctc gacatccacc tcggtgactt
     1381 cgaggtgctc cctgtcacag ctgaaaccgc caccgcatac gccagtgtgc gcgtcgcgct
     1441 caagcgagcg ggcacgccga ttccggcgaa cgacgcgtgg atagctgccc tcgctttgcg
     1501 gcaccgtttg ccggtgctca gtcgcgacgc ccatttcgac gttgtgctcg gtctccagcg
     1561 cgtcagctgg taggtccgac aacactcagc gtcttgaatc gacgctgagc gggcgaccag
     1621 ctttcccctc ggttcgatgc tttcgctcag ccgtcaaacg gtcaatgcgc tgctgcagga
     1681 cgtggaacag gagcaactag ggttttgtag gagaggtggt gtgcgtatcc tggatttccg
     1741 acgcctgttg gaattggaac tctcgatccg gtagcgaccg gaggtatgcg tcgaaacgca
     1801 gccggagacc acttcccagg cgccgggcag cgcgcgctgc ttggtcctgg acgagggcgg
     1861 atttgctgct ctggccttgt ccaagaacct attggaatga tggcggcggg ggtcggaatc
     1921 gaaccggcgt ccacggcttt gcagggggca tattatctca atgaaaccaa ggcttaacct
     1981 acctccccac ggcctcctcc acgcgatctc aaatcggacg acctggccgc tgtagcgcca
     2041 cgcgcattca cgcgaaatga agtcgctcat gatagcggct agggggaaaa gacttgtgaa
     2101 ggccgcctca acacgatccc ggcgccgcga cactttaacc cgcgcgggtg ccggtacgga
     2161 actcctccgc ccgcgcgatg ccgtctacgg gaatgactaa cgctatgaag cgcaagctca
     2221 ccgatactaa agttactgcg aaacagcgcc gcgagctgaa agcgctcgtc gcgcgtcccg
     2281 agaggcgcat cgatacctcg gacatcccgc cgttgacaga cgcgtttagg aagaacgcac
     2341 agcgcaatcc cttctaccgc gtttgcaaga cctccactac tgtgcgcatc gatgcggacg
     2401 tgctggcttg gctcaaatcc aagggtcgcg gctaccagac ccgtctcaac cacatcttgc
     2461 gcgacgcaat gctggcggag gtgaaatagc gccctcaggc gcagaaggaa ggcccagtca
     2521 acgaagacag atgtgattag ctattgacat ccggatagcc cgcatgcaac aaaatataaa
     2581 tatgaggtac acgcagtagc acgccctctg cgacacccaa agcacagatc tgaagcttac
     2641 gtccccaact gagaaccaac tcgagccgca atctgacttt gttccgtatc gtgttgcccg
     2701 gtgttcgagc attcaaccta tcgcacgctg tcgagggtga cttgggtcat gcccaacgcg
     2761 gcataagcta gtcgccccgc tgttgccgct gaggactttt ggtgcagagc ggtctaagct
     2821 agcctgtcag ctagatggac agataacatg gacgagtttg gtgcctcaga ggtcaagaac
     2881 aagctcgccg cgttgctcga cttggtcgaa caaggcaaag aaattaccat cacgcgctgg
     2941 ggtaagccgg tggcgaagct cgtgccggcc gggcccggct tcgaccgcga caaggcgcgt
     3001 gcagccgcgg cccagctcct cgaggcgagc cgcggagtca agctcggccg gctcaagctt
     3061 aaggatctga tcgccgccgg gcgtccatga gttttgtggt cgacagctct gttacgctca
     3121 gctggtgttt cgaggacgag cacacgcccg cgcgcttggc gctgcttgag cgtgtgactc
     3181 aggaggcgcg ctggcacctc agctgtggcc cctcgaggtg ttgaatgggc tgcagatggc
     3241 cgagcggcgc aagcgtctcg atgcgtcccg gcggcgacgt ctcgccgggt ttttgcagca
     3301 gctgcccatc agtctggacg tgcagacggc cgcgcaggcg tggggggcga ctgccccgct
     3361 agccgagcgc tttcggctca ccgtctaaga cgccgcctac ctggaagtgg cgcagcgtag
     3421 cgcgctggcg cttgccaccc tcgatcaaga tctgcatgcg gccgctctgc gaatgtcagc
     3481 tgcggggtcg aggccagaaa aaggaatcgc cctcggcaat ttccctcgtg cccccccacg
     3541 gcaatctctc agctcttaag cagcttcggg tgattctttt tcagccacga aacaacatcg
     3601 tcccgtaacc cctcggccaa cttgtacatc tcctgcgcct ccgccatgga gatgactccg
     3661 gcagtctcgt agccagtgat gttgcgtttc ttgcgaaaac gatcgaaccg accaactgcc
     3721 gctggtggcc agccgacggt gtatgcaagg ctttgaatgg tcctgtagtg atgctgctcc
     3781 cgcgtggcgc gatatcctgc cgcggccagc gcggcggttg cggcttggag cgcagcgttg
     3841 tatgcaatat tgagctgcca gtcttccgaa agcccggcaa ccttggcatt ggcgaggtcg
     3901 cgttcaacga tcgccagaag cccggcgatt tcctcagggc tcgtctcgtg ctccacaagc
     3961 cagctatttc ttagccaatc gcgcaagctc atgctcgtcc ccgatcagga agatcttctt
     4021 ggcccttagc acactacgca gaaaatgatg cttggccgcg agcttcgtgc gaaattcggc
     4081 cggtggatag acagtcggat taacctcgcg cccgattgcc ccctgtgcgc ccgccagtgc
     4141 ttcggacgct tcagcaaacg acacatcgcc caacaccata acgtccagat cactgccgcg
     4201 gcgctcggtc ccctgggcga cagagccgta gacaaacgca acgcgaatcc gaccagcgag
     4261 gggcgcaagc actgcccgaa gcgcatcggc gaccccggcc gtcttcacca ccaggccgcg
     4321 tagctcgccg aaaattgggc tttccgggtt cgcctgaaaa tacacttgcc gtccgcggac
     4381 tgtgcgccgc acaaccccgg cgcgcgcgag gaactcgagc tcgcgctgca ccgttccacg
     4441 cccaaggccc gcgagttgaa tcaggctctc ctggagatgc tcctgatcgg cacgcgtgta
     4501 tagcaacgcg agcaacgttt gccgcgtctt gccgaagagc gataccgatc cctctggggt
     4561 gatgccttga ctacccacca tgggtagcag gatacccatg gtgggtaggc gagtcaacgg
     4621 cgagcatgac gcgctgctgt tggcgatctg cggtgagccg cgccaatgac tcaattgacg
     4681 gaatagcgat cattggtcga tgctacgggg ccgcgcagac cgtcgcttac tggactccaa
     4741 tgcgaagttc tcgtattcct ccaccgcttt ggctcctgat cttcgctgta gcgatgtggg
     4801 cgttgaatca ctactacccg attgtgacaa taatttccga gccctggaat cggctgggct
     4861 ggtccgtgat tgcgattgcg ccacttgcgc ccatcgcagc gctcattcaa ttccggcgag
     4921 cacatacaac ggtgaacccg cataggcccg agatgacgac cacactcgtt acctccggcg
     4981 tgtatgcgtg gacgcgcaat cccatgtacc ttggtctgtc gattctgctt ctaggctggg
     5041 ccatcaagct cggcacgttg agcccatttg cgggaccgct gctctttatc ccgctcatcc
     5101 agcgggtcca aattcgtcct gaggagcacg cattgcgaat gcagttcggg aacgacttcg
     5161 accagtactg ccagcgtgtg aaccgttggt tcgggcgaac ctcacaggcc tgatcccctg
     5221 caactcttta accgagtgcg taatgaccaa ggataggcgc caaccgcagg gcttccgtcg
     5281 attcctcgta gttcttctgg ctgtcggctg tacgaccgcc tgggcgcaga ccaaacaaga
     5341 gcatgttcac caaatggggc ctagcgttat gccctttgat ctctccaaga cgacgcacgt
     5401 cttccgaatg accgaatccg gaggctgctc ccgacccaaa tcactcccgg attgatcgtg
     5461 cccgtcgttc caatgcccgt accggagttg ttggccgtcg gcgtggtgaa ctgtgcctcc
     5521 acgatgggta ttaaatgatt gaagaaagcg ggtaggtcga gatccacgac gctggattta
     5581 agatacggca tgctgtactg cagagtgctg taggttagaa actggggatt agccatggat
     5641 gtgggaaggc ctgtgtccgg gtctacgctg ttcgtggaag tgcgcgtagg aaacgaatag
     5701 ccgatctgcc cggtgagagc caaaggtcga gcccagccca tcttgtcagg aagatcacct
     5761 agccctttcc ggaacaacac cgacggggtg acggtgctga acgaatcgct gcccacggcg
     5821 ctcgcgccga tgccgcccca ctccacgttg atcgccgccg acagtgcgaa ttcgtgttcc
     5881 gcatccctta cgaactgata ttgaagggcg gtctcgagat tgtcgaagcc gctcgccctc
     5941 aagctgccag gcgtgctgaa ctgtgtccag ccggttccga tgctgatccc gaggttctcg
     6001 gtaatccgtt tgctgaattc gccggagatg tcgagctgag tcgtgggcgg gacatcgccg
     6061 ctcttggccc acgacaccgt aggtggcgac aattcgtctg cgacacacgg atcatcgatg
     6121 gtgagcgttg caggcagaaa ccgaatgccg acgaagcagt gcgcctgagc cgcggacgcg
     6181 tgtaggaggc ctgcaaggac cgctatcgca gcgccacgaa ggcccctagt aaccaggatt
     6241 cgcatacgcg cacactacat cggctattcg cgggcggaga tgcgacaaac tgtcgcagct
     6301 tgaaggctcg ccccgggtcc gagcagttta agtggtcaaa ccaaagcggc gaaggtctgc
     6361 ttcccaatag cgcgacaggc ggcaatgggt cgtgaacgac aaaaggcagc cgctgtcggc
     6421 gcatccttgg gatcatcggg tgtgtcgctg tttccggcca ctgcgtggtg atctggtcgg
     6481 gagtggggtg cgcctcacta ccgcccctcg agctcggtac ccgtgcgaag gcgttccaga
     6541 tacgcctggt acgcgaaggt gcggtcccgt gccttaccgc tcgtctcgac gaggacgccg
     6601 acgctctcca gcagttgaac ggctttaccc gcggtcggct tggttatctt cagtagcttc
     6661 acgatgcctg ggatcgttat caccgggtga acggggagct gctccaacag tcgagcggcg
     6721 acgaccgagg ccccgcttgc tgcgagcagg cttctacgat gggcagtgac gagcgcaaag
     6781 acgtcgcgcg cagcgacgac cgcttcatcc gcgatcgtgg ctacaccctc aagaaagaag
     6841 tccgtccagg cttcccagtc accgtgctca cggacctcgc cgagcagccg gtagtactcg
     6901 gcccggtgcc gcttgaaaaa caagctcagg tacagaagcg gtgccgacag caccttccag
     6961 tgctcgagca gcagcgtgat cagcagccgg ccgatacggc cgttgccgtc gaggtagggg
     7021 tggatggttt cgaactggac gtgcgccagg ccgatccgca ccaacggggg cagcgagtcg
     7081 gcggcgtgaa agtacttctc caggtcgccg agcagcgccg gaacctcctc aggcggcggc
     7141 gggacgaaga cggcgttgcc ggggcgagtg ccgccgatcc agttctggct gcggcgtacc
     7201 tcgcccggtt gcttgccggc gccgcgcacg ccacgcatga ggcggcggtg cgctgcgtga
     7261 agcagccgca tcgagaccgg aaggcctttc gagctcgcga gctgcgcgcg cgcatacttg
     7321 agcgcgtcga ggtagttgca gacctcgcgc acatcctcaa gaggttcgcg atggtcctgc
     7381 agcgcctcgt actcgaaaag atcgaccagc gtcgcctgcg tgccctcgat ctgcgaggac
     7441 acgacggcct ccttgcgcac aaaggcgtag atgaaccagt cgagcgaggg aaccatctcg
     7501 ccggcaacct caaggcgggc gaccgcttgt tctgcgcgcg caagaagctt cgctcgcttc
     7561 aggtccacct cccacggcgg gccttggggc ggaagcggat cgggcacgaa ggtggcgact
     7621 tcttcgccac ccactccgcg tcgtagagtt cggccagttg cacgcacgtt gggctccgct
     7681 gggaaagaat tctttaccag aaacagacgt tagtaaaggg tgcttttcta ggtggctttg
     7741 ctggcaaagt cggagcacga ccggcggaca ctggccgcgc cgttcgtcct gccgctgcag
     7801 ttcctgccga tgcgggcgct ggactcgggg cggcactccc ctctcctgct gcttggcaac
     7861 gtgcgggcct gtgggctggc cggcgaataa cccgagaaac tcgcgctatt cgatggtgct
     7921 ttttgtggcg gtaggacgag gcgatgtccg ctatctacga tttgagcggc cggattgcga
     7981 caacgtcaat gtctcaaacg ggtcggcagg gccaaaacgt gatcggtatc ggcgaggccg
     8041 cgtcggcggc cgcagtcgga catattgcgg tcgtctcgga ttccggattc cggattccgg
     8101 tttcccgttg cctatcagga ccggtcgtag ggccctgact taacctgacg ctgccgaagc
     8161 gacccgaccg gccatcaccg gctgttcgtg ccccgagtcc agaatgccca aaagcagacg
     8221 gtcgcactgc gccatcagtc gccaaactgt tgagtcgaga atcgattccg tgggctgccg
     8281 gttgtgcctg gaatgacagg gtagttgcac aagggtatgc tgttcaagta gccacgcacc
     8341 gcctcccctc cacgggataa ttccagccaa cggcggatac gggatgcatc ctcgggtcgc
     8401 gaagcccgtc tttccggtac accctgtacc gcttcgccag gcagcaacaa ttcaaaacca
     8461 gagaagggga gatgccatga attcattgtc gtcaggaaag tctctgccgc atcgtctatg
     8521 gaagaagctg atcctcggcg ctatgtgctt ctctgccgtc ttgattctta caccgctgac
     8581 atgggcccag gaaaagcgcc ccatagccgt ttcgggggaa ggtgcaaagg gtcgatacct
     8641 tcaacagtac attattgacg tagacgacac gccagggcat caaatccgca tcaacgagat
     8701 tcagcgcaca tacgctcctg ataagcaggc tgtgatcgag ggtgagcgaa tcgtcgagac
     8761 ttggtcgaga agcctcacga actacattag cgggatcggg cccgttttgg tcgcctacaa
     8821 cacgtgggta acggacaagg gaaacaagat atttatcgaa ggcacgggca ctagtgaatc
     8881 tcaaataacc gacaccggtt caaagcgcgg aacatatcat ggtacgtctc gattcgtcgg
     8941 tggcacgggt cgatttgcga aaatacgagg cactctagta gatgtgtcca catttgatac
     9001 tgatcccaaa tcgggctaca acacgatcga tagtcacggc caatactggt tcgaagagta
     9061 ggagaggcag ggagcattgc agcgtttcaa ttacaccaga gggaggagaa cagcatgaag
     9121 aggcttcaaa cgtggatgtc gctcgcctca gtggcgattt gtttgatcac gcctgtttcg
     9181 agtcgcgccg agagcgggac gatggatgtc cttatctcga ctgtttcggt ccttaccagc
     9241 atcccgatgg gagatacgac agttacagca aggagtggga gcgggacggt cacgtttatc
     9301 aaaagcagtg gcggtccatt tgttgaaggt gcgagcgggg ccgcgcaatt tgccagtttc
     9361 tcgaagaaat cagccaccgg tttcgatctg gaggcggatg gtctcgtgac gttctcagcg
     9421 gaagacaccc tgcagttgct cttcaaacgc agaacgggcg atctggcggc aggcacgtca
     9481 ggcgagggaa cactacagct tacgggcggg accggacggt tctcaggaat tagcgggcaa
     9541 tgcaaataca aggtggagaa cctttcaggc aactggcagg tcactatggc aaagtgtcag
     9601 tggagtcggt agcgaattgt tactcgggtc gcccacgcga gatcgctggc ctatttgtca
     9661 gagcagttct ctggctagtc taggaggaag atgaaatgac caaaaatgca tatggcttga
     9721 cccttcaacg aaggattctg tcgggcgttt ttctagtgat gctgtcgctt ttcgccttgc
     9781 ctgcattttc cgaagaagta ctgagaatcg tgagttttgt tgcagctggt ccggagcaac
     9841 aaaaggaact ccagaagatc gcgcccggga agctctacac ctctgcgaaa gggtgccggt
     9901 gggtcaagtt ctggtccgat cccgcgaccg gcgagactgg gtcggttagc ctttggaaga
     9961 gtcgcgctga cctagacgcg ttcttgaact ccgacgctta caaggcgata tttccaggca
    10021 aggtcagttc ccttgctaag ggcgatgtct ccgttaaagt ttacccggtg gtcaagccaa
    10081 aacattagga gacaaatccg agggcgagcc atgccttgtg agatcggaac actaagcgcg
    10141 catcgcggtg cgtctggagt tcattaagga gtcgagtgcg cttttcgttg ggcgacgtcc
    10201 gctcttcgag gcttcagcgg ccggattaca ctagtccgat ctcgagggga cgggacgcga
    10261 attatccaac ttcaacatca aacgggattc aacgaagcag agtgggagga agagtacggg
    10321 accaaaagct gagccattct gcagaagctg gctcgcatca gaacaaggag cagcgaccga
    10381 caaccacgct caactgccgc cggtaaagcc gagtcgcagg taacgttgat ggcatccggg
    10441 tgtgtgcgtg gtggccctaa tcaatatata cagcccaggg aggaattcat gaaagcgcaa
    10501 catgcatcaa tcaagatcac tctgcttctt ctggcgtcgt gcatgctctc atctgcattc
    10561 gcggcggacg ctgaacagac gtacctcgtg gcgtacgcga aattcaaacc agggaaggcg
    10621 ccagaagccc tcaagatcat tcacgagcat ttctggccgg tcgacaagaa agtggggcgc
    10681 aaggccatac cgttcgactt cctgactgga gagtgggatc acgttgtgta cttcccgtac
    10741 gacaccgcgc gcatggacac aattccccca ggcgcagaat ggtggaaggc tttagcggaa
    10801 caggaaggtg gcgctgagca ggcacagaga ctctttcaga gctatcttga tttggaagcc
    10861 aactccaaat ttgaggtagc gaaatcaccg gggcctctga tgagtacttc gagccgttga
    10921 ccaggccggg gccgcggacg ctctgttcgc ggctccgagc aagggccagc ttgcccgctg
    10981 caaggctgac tgagcgtcgg ctgccgggca aagcgtaggt ccggagtggg tcgaaccttg
    11041 ccgttggcat ctggcgactc tcagccgata cccgccttcc agtattcgat cagcaccaac
    11101 tgcgagatag acccgaagcg caaaatcaca acgcgccgtc catggaggaa gcggtgttag
    11161 ccagtgccat tctcttgcag cggaaactcg acgcgaatct ccacaccaga accgtcctcc
    11221 aggttccttg ccaaagcctg cgcaccgtga aactcggaga tcaggcgcac gatgtaaagc
    11281 cccaggccga ggtgggggtc ctgctgatct tgttccttgc gcaccgagac catcgactcg
    11341 aacagccggt cctgcatgtc cgcgggcagc ggtggtcccg cattcgtcac tgatagccgt
    11401 gcggccgaac cgtggcgctg aagcgcgaag tggatcgtgc tgtcctttgt actgaattcc
    11461 acggcattgg ccgcgagttt gtccagcatc tgggcaacca ggtccggtgc gccgttgacc
    11521 ggaaacggac caggtccggc atcgaatgcg aagcgccggt cgcgatgcgc aagccggtag
    11581 ccctcgacgc aaccgccggc tacctcggcc aaatcgaagc attgccgttc taccgacgcc
    11641 agcgattgtt cgagtcgcgt cgcttcgctc atgcgcgtga agatcgtgct gagccgggtc
    11701 aggccctctt ctgcccgatg caggtaaatc ttcgcctcgt ccggcagcgc gacctgtttg
    11761 aggttgtcta gcgaggagcg cacgaccgcg acgggagtac gtaactcgtg cgacagccga
    11821 ccacccagcg tttcgaggta gccggtgtat tgctgcagtc tttgcaggat gtccgcgaag
    11881 ctgcgcgaga ggtcgccaag ctcgtcggcg gcatcggagc caccgaccgt gccggtcacc
    11941 cgtccgtgcg cgtcgatgga gtgttcggct tcgtcgcgca gccgccggat tctcaccgag
    12001 agccgcgtgg cgaacaccag cagcccgatg gtcccgatgg tgaagacgac gatcagcgca
    12061 gcgaacagtt tttcgaaggc ccggttgcgc agcgcgagca cgtcgttcgt cgtttcctcg
    12121 accagcacgg cgcccaccac cttgtcctcg aggtagacgg gttgcgcggc cgagaggacg
    12181 accgcgcgcg aatccggagt caggcgccgc cgcgtaccgg tttgtcctgc cagcgcgtca
    12241 tcgatttcac ggccttcgag ccgataggtg ccgggcgcga tgtcctggaa gtcctcattc
    12301 ggttccgaca tggccagttt gaggatcggc ttgaccagca gatcggcccc ggcttcccag
    12361 aaacgcgtga tcgcgtcctg gtcgggatcg ggttgtacag gcggtgcccg cagtgacccc
    12421 gcttgcgcta tcacgcggtg gttcac
//
LOCUS       DASPUL010000619         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_118609, whole genome shotgun sequence.
ACCESSION   DASPUL010000619 DASPUL010000000
VERSION     DASPUL010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118609"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="VEJ00_14320"
     CDS             <1..1456
                     /locus_tag="VEJ00_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02387.1"
                     /translation="VARWLARHLPRNVEIAWDGYEEERAMAAAWPRFMPLLEEDADVE
                     ANIPWRRWLDAARGRAGELEWLIRQFEGLAVNERKRAELYDSLRLPLRWELGNLKLSR
                     TRNWARPRPFYYHSEPLVTRSQVSLEQELVRPAPGLTKLSRREGESVLRAVREVMLVR
                     YRELYGTTLGDPGSVVRAEMGRGVVIHLWNLPPSRRLPLRAYVAGFTLKNGVPINYIE
                     AIGLCEWIEVGFNTFYTYRQGETAWIYAQALRCLCAFTAATTISVYPYQIGQNNDEAL
                     DSGAFWFYRKLGFRSGRADLEELARREEQRIAADREYRTPRKTLKRLAEAHVFYELQG
                     SECGAWDTFSMRNLGLRVNRRMAREFGGDAARIREASVREVTRALRVNPAGWSADQQR
                     SLENWALVLGLIADLEKWSAREKEKLIRIIRAQSGRSEMHYLRSTRRHERLRRELLQL
                     GHPCSPGSRQETDLPQRSLRTAAKDAKKGWSVRG"
     gene            1488..1799
                     /locus_tag="VEJ00_14325"
     CDS             1488..1799
                     /locus_tag="VEJ00_14325"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXX02388.1"
                     /translation="MKTVSRIFRALADPTRRQILQELKGCELAAGDIAARFPISGPSI
                     SRHLAILKGADLVSERRQGNRIFYRLEPDRIAGAMGDFLSTVCPTQMLHRRRTRKERK
                     A"
     gene            1796..2728
                     /locus_tag="VEJ00_14330"
     CDS             1796..2728
                     /locus_tag="VEJ00_14330"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HXX02389.1"
                     /translation="MKIAITGGTGFVGGHLARHLLAERHEVVLISRHPTVEQTGMGAA
                     PVASDLSDPGVLAAAFAGCEAVAHCAGINREIGSQTFQRVHVAGTRNVVEAGRRAGVR
                     RIALMSFLRARPDCGSPYHESKWAAEEIVRQSGLDYTIVKAGMVYGRGDHMLDHLSHA
                     FYTLPLLATVGFRQKAIRPLAIDDLVPILQAALVDSRLPGKTVAVTGAEELYLSDAAR
                     RVANVIGRPAWILPAPILFHRVLAQICEWTMKIPLVAKAQVRILREGVVEPATTCDPL
                     PADLVPVRRFTSDQIRRGLPEPGPFGLRDLRCCA"
     gene            2725..>3103
                     /locus_tag="VEJ00_14335"
     CDS             2725..>3103
                     /locus_tag="VEJ00_14335"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HXX02390.1"
                     /translation="MTDQDEAGTGETKPDGLTAHPILLYDGVCGLCNRSVQFILRHDR
                     KARFRFAALQSTFAAPILRRHGADPAELDTVYVVVNHEPTKQDQPEEALLARSDAVIF
                     VLRQLGGVWQALAVLLRFIPRPVR"
BASE COUNT          566 a          897 c         1045 g          595 t
ORIGIN      
        1 ggtggcgcgg tggctcgcgc gacaccttcc gcgcaatgtt gaaatcgcat gggacggcta
       61 cgaagaagaa cgcgcgatgg cggctgcatg gccgcggttc atgcccttgc tggaggagga
      121 tgcggatgtc gaggccaaca tcccgtggag acgctggctc gatgcggcgc gcgggcgggc
      181 gggggagctg gagtggctga ttcggcagtt cgaaggcctg gcggtcaacg aacgcaaacg
      241 ggccgagctg tacgactcgt tgcgtctgcc actgcgctgg gagctgggaa atctgaaact
      301 ctcgcgcacg cggaactggg cgcggcctcg ccccttctac tatcacagcg agccgctggt
      361 cacgcgcagt caagtctctt tagaacagga gttagtccgg ccagcgcccg gtttgaccaa
      421 gctgtcccga cgcgaagggg agtccgttct gcgcgcggta cgcgaggtga tgctggtgcg
      481 ctaccgcgaa ctctacggca ctacgctcgg cgatccgggc tcggtggtgc gggcggaaat
      541 ggggcgcgga gttgtgatcc atttgtggaa tctgccgccg tcacgccggt tgccgctgcg
      601 cgcctacgtg gccggattta ctctgaagaa cggcgtgccc atcaactaca tcgaggcgat
      661 cgggctgtgc gaatggattg aagtcggctt taacaccttc tatacgtatc ggcagggcga
      721 gaccgcgtgg atctatgcgc aggctctgcg gtgtctgtgc gccttcacgg ccgcgacgac
      781 gatttcggtt tatccctacc agatcgggca gaacaacgac gaggcgcttg actcgggcgc
      841 gttctggttc tatcgcaaac tgggatttcg ctccggccga gccgatcttg aggaactcgc
      901 gagaagggaa gagcagagga ttgccgccga tcgggaatac cgcacgccgc ggaagacgtt
      961 gaagcggctg gcggaggcgc atgtgttcta tgagctgcag ggaagcgagt gcggcgcctg
     1021 ggacaccttt tccatgcgca atcttggact gcgggtaaac cggcgcatgg cgcgggagtt
     1081 cgggggagac gcggcgcgca tccgggaagc ttcggtccgg gaggtcacgc gagcgctgcg
     1141 cgtgaatccc gccggctgga gcgcggatca gcagcggtcg cttgaaaact gggctttagt
     1201 cctggggctg attgcagacc tggaaaaatg gtccgccaga gagaaggaga aattgattcg
     1261 gatcatccgg gctcagtctg gccgcagcga gatgcactat ctcagatcga cgcgacgcca
     1321 cgagaggctc aggcgggaac ttctgcagct cgggcatccc tgttcccccg ggtcgcggca
     1381 ggagacagat ttaccgcaaa ggtctctaag aactgccgca aaggacgcga agaaaggctg
     1441 gagcgtccgg ggttgacact atttagcaaa atagctaaac taggaatatg aagacggtct
     1501 cacggatctt ccgcgcgctg gccgacccga cccggcggca aatcctgcag gaattgaaag
     1561 gatgtgagct cgcggcgggg gacattgccg cgcggttccc catttctggt ccttcgatct
     1621 cgcgccatct ggctatcctc aagggcgccg atctcgtgtc ggagcgccga caggggaacc
     1681 ggatttttta tcggctcgag ccggaccgga tcgccggggc catgggagat tttctcagca
     1741 cggtttgccc gacgcagatg ctgcatcgcc gtcggacccg gaaggagcgg aaggcatgaa
     1801 gatcgcaatt acgggcggaa ctggctttgt cggcggacat ctggcgcgcc accttctggc
     1861 cgagcgacat gaggtagtgt tgatttcccg acatccgacg gtcgagcaga ccggcatggg
     1921 tgcggcacct gtggccagcg acctgtcgga ccccggcgtt ctggctgccg cctttgccgg
     1981 ctgcgaggcg gtcgcgcatt gcgccgggat caatcgcgaa atcggcagcc agacttttca
     2041 gcgggttcac gtcgcgggca cgcggaatgt ggtcgaggca ggacgccggg cgggcgtgcg
     2101 ccgtatcgcg ttgatgagtt tcctgcgggc ccgtccggat tgcggttcgc cctaccacga
     2161 atcgaagtgg gccgccgagg agatcgttcg tcagtcaggg cttgactata cgatcgttaa
     2221 ggccggcatg gtctatggcc gcggtgacca catgctggat cacctcagcc acgcgtttta
     2281 tacgctgccc ctgctggcga cggtggggtt tcggcaaaaa gcgattcgcc cgctggcaat
     2341 tgatgacctt gttcccattc tgcaagcagc gttggttgat tcccgtctgc cggggaaaac
     2401 ggtcgctgtt accggggcag aggagctcta tctcagcgat gctgcacgcc gcgtggcgaa
     2461 cgtcataggg cgcccggcgt ggatcttgcc agcccccatc ctgtttcacc gcgttcttgc
     2521 ccaaatctgt gagtggacga tgaaaatccc tctagtagct aaagctcagg tgcgaattct
     2581 gagggaggga gttgtagagc cggcaacaac ctgcgaccca ctcccggcgg atctggttcc
     2641 cgtgcgccgc ttcacatcgg atcagattcg gcggggcctg cccgagccgg gacctttcgg
     2701 gctgcgcgat ttgcggtgct gcgcgtgaca gatcaagacg aagccggcac aggcgaaaca
     2761 aaacccgatg ggctgacggc tcacccaatt ctcctttacg acggcgtctg cgggctatgc
     2821 aaccggtcgg tgcagttcat cctgcgacac gaccgcaagg cccgcttccg ctttgctgcg
     2881 ctgcagagta cgttcgcggc tccgatcctg cgtcgccacg gcgcggatcc tgccgagctc
     2941 gacacggttt acgttgtggt gaaccatgag ccgacaaaac aggatcaacc ggaagaggcc
     3001 ctgctggccc ggtccgacgc agtaatattt gtcctccgcc agcttggtgg ggtatggcag
     3061 gcgctggcgg ttctgctgcg gttcatcccg cgacctgttc gcg
//
LOCUS       DASPUL010000620         4460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_954867, whole genome shotgun sequence.
ACCESSION   DASPUL010000620 DASPUL010000000
VERSION     DASPUL010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4460)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4460
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954867"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VEJ00_14340"
     CDS             complement(<1..250)
                     /locus_tag="VEJ00_14340"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HXX02391.1"
                     /translation="MNAEIIAVGSELLTPYRMDTNSLYLTDQLNRLGVDVIFKSIVGD
                     NLRLLVAAAQHGLFRSDILLFSGGLGPTEDDLTREAVAR"
     gene            complement(408..1466)
                     /locus_tag="VEJ00_14345"
     CDS             complement(408..1466)
                     /locus_tag="VEJ00_14345"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HXX02392.1"
                     /translation="MGFSERILGRKNVNGGPRIEAVVPAAALAGGEIRITGSGLRPQE
                     LRRPRVQFGSVEGSLVVSSDAFLVARVPEGATSGPLVVATDGHVSNPHNVKVAVPIAE
                     NLHPVANPALDAEGNIYATFSGPRGQKVPVAIYKIDTNYAMKPFVVEMMNATSIAFDR
                     QGQMYVSSRHDGAVYRVAPNGTMTTYAEGLGVATGIAFDREGSLYVGDRQGTIFKVAP
                     DQQMFVFATLEASVSAYHLAFSPRGDLFVTGPTTSSFDRVHRIDPNGTVSTFYKGLGR
                     PQGIAFDVDGNLYVAASLAGKRGIVKLTPDAQASLEVAGQGLVGLAFAPGKSAVLATT
                     NAVHHLSWNIQGLPLLPE"
     gene            complement(1475..2011)
                     /locus_tag="VEJ00_14350"
     CDS             complement(1475..2011)
                     /locus_tag="VEJ00_14350"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HXX02393.1"
                     /translation="MTDAPDRSAGIPPAVPRTSRVRLPFWATLIATFCGIGRLHPGPG
                     TWASAVTVVLWAAIAHDLSPALRTPVVAGLAILVTLIGIPAATQVARACGAKDPQFVV
                     IDEVAGQLIALIAVPLAWKTFLAGFILFRAFDIVKPPPVRQLERLPEGTGIVLDDVAA
                     GVYALVGIHLLLHFGLLS"
     gene            complement(2008..2220)
                     /gene="yacG"
                     /locus_tag="VEJ00_14355"
     CDS             complement(2008..2220)
                     /gene="yacG"
                     /locus_tag="VEJ00_14355"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HXX02394.1"
                     /translation="MPRKRVVKLRCPICKKAVESTSAEFPFCSERCRTIDLGKWASGG
                     YVISSPVRDAEEPIREVNPEDPEGKP"
     gene            complement(2276..3769)
                     /gene="rimO"
                     /locus_tag="VEJ00_14360"
     CDS             complement(2276..3769)
                     /gene="rimO"
                     /locus_tag="VEJ00_14360"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HXX02395.1"
                     /translation="MKLDRSTITASPQDNSRTRPRQKVGFVSLGCPKNLVDSEVMMGL
                     LARAGAELTPRAEEADVIVVNTCSFIESAQQESVNTILEMAGHKNGGKAKKLVVAGCL
                     VERFRDQIRKDIPEVDAVVGTGELENILVAAGVGAPHVPRNGSSSPLVVLNSRPEGHA
                     RAAQGRFSRESWEGAVADLPNYLYDDATPRVLATPAHRAYIKIAEGCDHPCTFCIIPQ
                     LRGQFRSRRFESVVAEAERLAHSGVREITLIGQDTTCYGEDFGLKDGLALLLEKLAGI
                     DDLRWIRFLYAYPNKITGKLLDTIARHEKICSYMDVPLQHASPSALKRMKRGGGADVF
                     LRSIAKMRRTVPDLTLRTSFIVGFPGETEKEFEELCDFVREAQFDWMGTFAYSDQEGA
                     AAFGLEKKLPPREIERRRKHLMAIQRQISRKKKKALVGQEYDLLLEGESEDTDLLLEG
                     RTARHAPEIDGKVFVNDFPEEITPQPGCFYRCQITEAHDYDLVAKIL"
     gene            3940..>4460
                     /locus_tag="VEJ00_14365"
     CDS             3940..>4460
                     /locus_tag="VEJ00_14365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02396.1"
                     /translation="MSGKRVFIATVAALALLTTLAAAQDEKNEVGGTIGRIFVSDQGI
                     NRTIFDPIIHSGKGLTLEGFFAHRFLVTPVYSIAGEVPVAINVDEDLNAGAFNQAAVP
                     TDYKSLFVTPGARVNLFPTTAVSPWVSLGLGFGHFSEGNMLVFGGANPGKSTTNFVLE
                     GGLGLDVRVWSRL"
BASE COUNT          868 a         1305 c         1423 g          864 t
ORIGIN      
        1 ccctcgcgac cgcctcgcgg gtcagatcat cctcggtggg gccaaggccg ccgctgaaga
       61 ggagaatatc ggagcggaac aggccgtgtt gggcggcagc tacgagcagc cgcaaattgt
      121 cgccaacgat gcttttgaaa atgacatcga cgcccaggcg gttgagctgg tctgtgaggt
      181 agagcgaatt ggtgtccatg cggtaaggcg tgaggagttc agaccccacc gcgatgatct
      241 cggcattcac gtgcttagaa gtctaaatga aaacgcctgg tcggcggatg gaaccacgag
      301 tcggcagcgg ccgcggggca gaaaagctgg ttcctcctcc ccctcgactt cgctcggggc
      361 tcgccttacg gctcggcaga gtcgctcgaa atgacaagaa tgggggacta ttccggcagc
      421 agcggcaggc cctgaatatt ccacgacaga tgatgcacgg cgttcgttgt ggccagcacc
      481 gcggactttc ccggggcaaa tgccagtcct accaggccct gacccgcaac ttcgaggctg
      541 gcctgagcgt cgggagtgag tttcacgatg ccacgcttgc cggcaagaga tgctgcgaca
      601 tacaggttgc cgtccacgtc gaaggcaatg ccctgcggcc ttcccaggcc cttgtagaag
      661 gtgctgaccg tgccgttcgg atcaatcctg tgaacacggt cgaagctgga agtcgtcggc
      721 ccggtcacga acaggtcgcc gcgcgggctg aaggccaggt gataggcgga gacgctggct
      781 tcgagcgtgg caaagacgaa catctgctgg tcgggagcaa ctttgaagat ggttccctgg
      841 cggtcgccga cgtacaggct gccctcgcga tcaaacgcga tgcccgtggc cactcccaag
      901 ccctcggcgt aggtggtcat agttccgttg ggcgcgacgc gatacacggc gccgtcgtgg
      961 cgcgaagaga catacatctg tccctggcgg tcgaaggcga tggaagtggc gttcatcatc
     1021 tcgacgacga acggcttcat ggcgtagttg gtgtcgatct tgtagatcgc gaccgggacc
     1081 ttttgccctc gcgggcctga gaatgtggcg tagatgttgc cttcggcgtc gagcgcgggg
     1141 ttggcgaccg gatggagatt ttctgcgatc gggacggcca ctttcacatt gtgaggattg
     1201 ctgacgtggc cgtcagtcgc aaccacgagc ggtccggaag tcgctccctc aggcacgcgc
     1261 gcgaccagga acgcgtcgga gctgacgacg agcgatcctt ccacactacc gaactggacc
     1321 cgggggcggc gcagttcctg cggacgcagt cctgagccgg tgatgcggat ttcgcctccg
     1381 gccaaggccg cagccggcac gaccgcttca atgcgcgggc cgccgttgac gttctttctg
     1441 cccaggatgc gttcggagaa tcccatgggg agtactaact cagtagacca aaatgtagca
     1501 gaagatgtat cccgacaagg gcgtaaactc ctgcggcgac atcgtcgagc acaatcccgg
     1561 tgccttcagg aagtcgctcc agttggcgca cgggaggtgg cttgacgata tcgaacgctc
     1621 taaaaagtat aaaacccgcg agaaaagttt tccatgcgag cgggacggcg atgagcgcga
     1681 tcaattgccc ggcgacttca tcgatgacga caaattgggg gtcttttgcg ccgcaagcgc
     1741 gcgctacctg ggtggccgcg ggtatcccaa tcagcgtgac caggattgcc aggccagcga
     1801 cgactggcgt tcgaagcgcg ggagacaggt catgggcgat ggcggcccac agcacaaccg
     1861 tgacggccga tgcccaagtc ccgggcccag ggtgcaggcg gccaatgccg cagaaagtag
     1921 caatcagggt cgcccagaaa ggaaggcgga cgcgagacgt cctcgggaca gccggcggga
     1981 tgccagcgct acggtcgggc gcatctgtca cggtttgccc tccgggtctt cgggattcac
     2041 ctcgcggatt ggctcttcgg catctcgcac gggcgaggag atgacatatc cgccactggc
     2101 ccacttgccc agatcgatcg tgcggcagcg ctcgctgcag aaagggaact ccgcgctggt
     2161 gctctcgacg gctttcttgc agatagggca gcgaagtttg acaacacgct tgcgcggcat
     2221 ggagattctc tcattgtaga aggaagattt tcagattgca gattgaaaaa ccgcgctaga
     2281 ggatcttcgc taccaaatcg tagtcgtggg cttcagtgat ctggcagcgg tagaagcagc
     2341 cgggttgcgg cgtgatttct tccgggaagt cgtttacaaa cactttgccg tcgatttcgg
     2401 gcgcatgcct ggcggtgcgg ccttcgagca acaggtcggt atcttccgat tcgccttcga
     2461 gcagcaggtc gtactcctga ccgacgagcg ccttcttttt cttcctgctg atctggcgct
     2521 ggatcgccat cagatgcttg cggcggcgct cgatttcgcg aggcggcagc tttttctcca
     2581 gaccgaaagc cgcggcgcct tcctgatcgg agtaggcgaa cgtgcccatc cagtcgaact
     2641 gggcctcgcg cacgaagtcg cagagttcct cgaattcttt ctcggtctcg ccgggaaagc
     2701 cgacgataaa cgacgtgcgt aaggtgagat ccggcaccgt gcgccgcatc ttggcgatcg
     2761 atcgcaggaa aacgtcggcg ccaccgccgc gcttcatgcg cttcagcgcc gagggcgagg
     2821 cgtgctgcag ggggacatcc atataggaac aaatcttctc gtggcgagcg atcgtgtcga
     2881 gcagcttgcc ggtgattttg ttgggatagg catagaggaa acggatccag cgcaaatcgt
     2941 caattccggc gagcttttcg agcagaaggg caaggccgtc tttcaggccg aaatcttcgc
     3001 cgtagcaagt cgtgtcctgg ccgatcaagg tgatttcgcg gacgccggag tgcgccaacc
     3061 gctcggcttc ggcgaccacc gactcaaagc gccgcgagcg gaactgaccg cgcaactggg
     3121 ggatgatgca aaaggtgcag ggatgatcgc agccttctgc aatcttgatg taggcgcgat
     3181 gcgcgggagt ggccagcacg cgcggcgtgg cgtcgtcgta gagataattg ggcaggtccg
     3241 cgaccgcgcc ctcccacgac tcgcgcgaga agcggccctg ggcggcgcgg gcatgaccct
     3301 ccggccgcga gttcagcacc acaagcggag aactcgaccc gttgcgcgga acgtgcggtg
     3361 cgcccacgcc cgcggcgaca agaatgttct ccagctcgcc cgttcctacg accgcgtcga
     3421 cctcgggaat atctttccgg atctgatcgc gaaagcgttc gaccaggcat ccggcgacga
     3481 cgagtttctt ggctttgcca ccgttcttat ggccggccat ctcgaggatg gtgttcaccg
     3541 attcctgctg cgcgctctcg atgaaggaac aggtattgac cacgatgaca tcggcctctt
     3601 cggcgcgggg agtcagttcc gccccggcgc gtgccagcag gcccatcatg acctcgctgt
     3661 cgaccaggtt cttggggcag cccaggctca cgaagcctac cttctgccgg ggtcgggttc
     3721 gagaattgtc ctgaggcgaa gcggtgatcg tggagcggtc caacttcatg ggatgttccc
     3781 atcttagcat ccaacggtcc gcgatcctgc atcgcagcgc atgcccacgg ccccggctag
     3841 gatcataaaa gatatacttg ctgggttcaa aaccccggct ggcgtgctag gctatgcctt
     3901 accgccgtaa tgactaccag gtgcctcgca ggaggagaaa tgtctgggaa acgagtgttc
     3961 attgcgaccg tcgccgcact ggcgctgtta actaccttgg cggccgccca ggatgagaag
     4021 aacgaggtcg gcggcaccat tggccgtatc tttgtcagcg accagggtat caatcggacc
     4081 atcttcgatc ccattattca ttccggcaaa ggtctcacgc tggagggatt tttcgcccac
     4141 cggttcctgg ttacgccagt ctattccatc gccggagaag tgccggtggc aatcaacgtg
     4201 gacgaagact taaacgctgg cgccttcaac caggcagccg tacccaccga ctacaagtct
     4261 ttgttcgtga ccccgggagc tcgagtgaac ctgtttccca ccaccgcggt ttcgccctgg
     4321 gtgagtttgg gccttggttt cggccacttc agcgagggga acatgctggt attcgggggc
     4381 gccaatcccg gcaagtcgac cacgaacttc gtcctcgaag ggggtttggg cctggatgtg
     4441 agggtctgga gtcgcctaag
//
LOCUS       DASPUL010000621         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_799936, whole genome shotgun sequence.
ACCESSION   DASPUL010000621 DASPUL010000000
VERSION     DASPUL010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799936"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VEJ00_14370"
     CDS             <1..981
                     /locus_tag="VEJ00_14370"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HXX02397.1"
                     /translation="VHPTAGATVVGARKFLIAYNVFLNTPDVEIAKKIAKAVRFSNGG
                     LRFVKGAGFLVRGLAQVSMNLTDFEQTPVHRVFELVKREAARYGVMPVSSEIVGLIPK
                     KALEQAAEWFLQVENFDSSLILENRLAAVMSGKMAVGGLRAGVEPFVEQLAAPTATPG
                     GGSASAAAAAMASGLATMVASMSRGKKAYLQYESQLSAAIARLAPLREELKAAIDADA
                     ASYNSVMKAYKDAKTAADEKAGKALVESALKEATSVPLSVAEKAFEIAGITQSLESVT
                     NPNMKSDLTTALALARAAVTGALANVEINLASLTDQAFAAEVRRRVSALS"
     gene            1173..2105
                     /locus_tag="VEJ00_14375"
     CDS             1173..2105
                     /locus_tag="VEJ00_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02398.1"
                     /translation="MRLFKICLASLLLVSVAYAMPLGSSARTCIPADLLQLISVDYRA
                     LKDSPTAMALKQQLMPDNIKQFEAALKNIGIDPEKDVDTLTFASFRSGKQGVRGIGVA
                     SGPFNMKSVLKKMKLEKVTPKKYGNSLIYPMDGGLLMSFLDDSSLLFGDPTAIRAALD
                     TRDGQSLGLDTNGNMADMMTSVDSAPVWSILDQQGTQNMLLSALGDAAKVGDYDTLKK
                     RLLGSRYTMNFSSGVNFDLTVLTSDSMTAGTLSSLVKAGMLYKKMSATPAEKVAVDNT
                     TVDSDSSNLQLHFKANDQQFQSLMHSQLFAAVSH"
     gene            complement(2178..>3233)
                     /gene="glmU"
                     /locus_tag="VEJ00_14380"
     CDS             complement(2178..>3233)
                     /gene="glmU"
                     /locus_tag="VEJ00_14380"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HXX02399.1"
                     /translation="ETIGHLRNFHLEEQAAMTLLTADLAEPTGYGRVLRKKAGSAEVQ
                     AIVEERAASAAQKKIREINSGFYVFDVRQLYANIGELSTDNPHGEYYLTDMAGVLREA
                     RQRVVAWKTANAGEVLGGNTRAELADIDHYMRMAKCRQLMADGVTIYYPATCVIDADV
                     EIAADTVIEPYVQLLGKTRIGTASRVRSYSVIRDSEIGDGVMILPGCVMDEARVGSTA
                     IIGPYSRLRPGSEIGEGAHLGNFVETKKIKMGKGSKANHLTYLGDAEIGAGVNVGAGT
                     ITCNYDGVHKHKTVIGDRAFIGSDSTLVAPVSVGNGAYVAAATCVTEDVPDDALAIGR
                     ARQVVKEGWAREKKLKK"
BASE COUNT          626 a         1022 c          926 g          659 t
ORIGIN      
        1 gtgcatccta cggcgggcgc gacggtggtt ggggcacgca agttcctcat cgcttacaac
       61 gtctttctca atacccccga cgtcgagatc gccaagaaga tcgccaaagc cgtgcgcttc
      121 tcgaatggag gcctgcgttt cgtcaagggt gccggttttc tggtgcgcgg cctggcccag
      181 gtttccatga acctcaccga cttcgaacag acgccggtgc atcgcgtctt cgagctggtg
      241 aagcgcgaag ccgcgcgcta tggcgtgatg ccggtgtcga gcgaaatcgt gggactcatc
      301 cccaagaaag cgctggagca ggcggctgag tggttcctgc aggtcgagaa cttcgattcc
      361 tcgctcattc tggaaaaccg cctggcagcg gtgatgagtg gcaagatggc ggtgggaggc
      421 ttgcgcgccg gagttgagcc ctttgttgag caactggccg cgcccacagc cactccagga
      481 ggcggcagcg cctcagccgc agcggctgcc atggcctccg ggctcgcgac gatggtcgcc
      541 tccatgtcgc gtggcaagaa ggcttatttg cagtacgagt cccagctcag cgcggccatc
      601 gctcgcctcg ccccgttgcg cgaggaattg aaggctgcta tcgacgccga tgctgcctcg
      661 tacaactcgg tcatgaaggc ttacaaggac gccaaaactg ccgccgacga aaaagcaggt
      721 aaggcgctcg tcgaatccgc gctcaaggaa gctacttcgg ttcccttgag cgtagccgaa
      781 aaggcgtttg agatcgccgg catcacgcaa tcgctcgaat ccgtcaccaa tcccaatatg
      841 aaatccgacc tgaccacggc gttagccctg gcccgtgccg ctgtcactgg tgcgctggcc
      901 aacgtggaaa tcaatctggc atcgctgacg gatcaggcct tcgccgcgga agtccgccga
      961 cgagtgtcag cgctgtcgta gagcggtgga agcgccacgc gtggtcggaa ggcctgcccg
     1021 tctctaatcc atcaacggtc actgtacttt tggtttgtca cccgtgattg caagtaaaat
     1081 agaataaagc gatcaacgaa ctacagggca agaacgtgca tccaacctag gagagcatgg
     1141 gatcctgtag tttctatgac ttccgaggcg ttatgagact tttcaagatt tgcctggctt
     1201 cccttctgct ggtgagcgtg gcttatgcca tgccgctggg ctcttccgcg cgcacctgca
     1261 ttcctgccga tctgttgcag ctgatctcgg tcgactaccg cgcgctgaag gattcgccca
     1321 cggcgatggc gctgaagcag cagctcatgc ctgacaacat caagcagttc gaagctgccc
     1381 tgaagaacat cggcatcgat ccggaaaaag acgtcgacac tctcacgttt gcctcgttcc
     1441 gcagcggcaa acagggcgtc cgcgggattg gggtggccag cggcccgttc aatatgaaga
     1501 gcgtgctcaa aaagatgaag ctggagaagg tgacgcccaa aaagtacggc aactcgctga
     1561 tctatccgat ggatggcggg ctgctgatga gctttcttga cgacagctcc ctgcttttcg
     1621 gcgatcccac ggccatccgg gccgcgctcg acacgcgcga cggccagagt ctgggtctgg
     1681 acaccaacgg caacatggcc gacatgatga ccagcgttga ctcggcgccg gtgtggagca
     1741 tcctcgacca gcaaggcacg cagaacatgc tgctatcagc cctgggcgac gccgccaagg
     1801 ttggcgacta tgacacattg aagaagcgcc tcctcggttc acgctacacg atgaatttca
     1861 gcagtggcgt gaacttcgac ctgacggtgt tgacctcaga ctcgatgacc gcgggaacct
     1921 tgtcctcgct ggtcaaggcc gggatgctgt acaagaagat gagcgccacg ccggccgaga
     1981 aggtcgcagt ggacaatacc acggtcgatt ccgacagctc gaacctgcag ttgcacttca
     2041 aggccaacga tcaacaattc cagtcgctga tgcattcgca gctgttcgca gccgtttcgc
     2101 actaacctcg aaccctggcg cggcgggcgc ctcgtccgcc gccccgaagg aatgcttccg
     2161 gactacaccg tccctgctta cttcttcagc tttttctccc gtgcccagcc ttctttcacc
     2221 acctgccggg cgcgcccgat cgccagtgca tcgtcgggaa cgtcctcggt aacacacgtg
     2281 gctgctgcta cgtaagctcc gtttccaacc gacaccggcg ctaccagtgt agagtcgctg
     2341 ccaatgaagg cccggtcccc gattacggtc ttgtgcttgt ggacaccgtc gtagttgcag
     2401 gtgatcgtgc ccgcacccac gttgacgccg gcgccgattt cggcatctcc cagatacgtc
     2461 aggtgattgg ccttcgagcc cttgcccatc ttgatcttct tggtctcgac gaaattcccg
     2521 aggtgcgcgc cttcgccgat ctcgcttccc ggccgcagcc gggaatacgg cccgatgatc
     2581 gcggtgctgc ccacgcgggc ctcgtccatc acgcagccgg gaaggatcat gaccccatcc
     2641 ccgatctcgg agtcgcgaat caccgagtag gagcggacac ggctggccgt accgatccgc
     2701 gtctttccca gaagctgcac atagggctcg atcaccgtgt cggcagcgat ctccacatct
     2761 gcatcaatca cgcaagtcgc cggatagtag atcgtgacgc cgtcggccat gagctgccgg
     2821 cacttcgcca tgcgcatata gtgatcgatg tcggccaact cggcccgggt gtttcctccc
     2881 aggacttccc cggcatttgc cgtcttccat gccaccaccc gctgccgcgc ctcgcgcagc
     2941 actcccgcca tatcggtcag gtagtactcg ccgtgaggat tgtccgtgga gagttccccg
     3001 atgttggcgt aaagctgcct cacatcaaag acatagaaac cggaattgat ctcgcgaatt
     3061 ttcttctgcg ctgcgctggc tgctctttct tccacaattg cctgcacttc ggcactgccc
     3121 gccttcttcc tcaagacacg gccgtatccg gtgggctcgg ccaggtcggc cgtcagcagc
     3181 gtcatcgccg cttgttcttc cagatgaaaa ttgcgcaggt gcccgatcgt ctc
//
LOCUS       DASPUL010000622         6445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1488705, whole genome shotgun sequence.
ACCESSION   DASPUL010000622 DASPUL010000000
VERSION     DASPUL010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488705"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VEJ00_14385"
     CDS             <1..460
                     /locus_tag="VEJ00_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02400.1"
                     /translation="LEEGADIAIGSRWLRAETQTQRQPLYRQAFGRIFNLLLRLTLGL
                     NFKDTQCGFKAFKQAGAQSIFPLQKIDRWGFDPEILFLARKCGFRVREVAVAWGHSGG
                     TRINPLKDGSRMFLEMLRVRWYDWTGKYAGNPGPRAQAGQSLPTGRGPRA"
     gene            complement(474..1589)
                     /gene="leuB"
                     /locus_tag="VEJ00_14390"
     CDS             complement(474..1589)
                     /gene="leuB"
                     /locus_tag="VEJ00_14390"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXX02401.1"
                     /translation="MQLKLTILPGDGIGPEVTEQAVLVLQAIADAFGHQLQLERKPIG
                     GAALAVANDPLPADTIRACLSSAAVLLGAVGSPAFDHNPSHLRPEAGLLRLRRELGAY
                     ANLRPAVFFPALEDCSPLRAELVRGTDIMIVRELLGGAYFGQPRSIEGSPGSRVAVNT
                     MRYGESEIERIAHVAFDLAMKRRRKVLSVDKANVLECSRLWREVVTRVGKDYPEVKLG
                     HQYVDSAAMLLVQRPTDFDVVLTENMFGDILSDQAGGVVGSLGLLASASIGGPVGLYE
                     PVHGSAPDIAGKGIANPLGAILSAAMLLRHSFHLEAEAAGIERAVSTVLSQGARTADL
                     AGKTHRAISTVEMGQCVIEAVKSGANPIPGQQRSQHR"
     gene            complement(1704..3089)
                     /locus_tag="VEJ00_14395"
     CDS             complement(1704..3089)
                     /locus_tag="VEJ00_14395"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HXX02402.1"
                     /translation="MATERARVTVFDTTLRDGEQSPGCSMNQQEKLRLAHQLDRLGVD
                     VIEAGFPIASEGDFESVKAIAAVVRRPVIAGLARACREDIERAWEALKDAAHPRIHVF
                     LATSDIHLQYKLRITRDQCVAQTRDAVRFAKSLCADVEFSPEDATRTEPDFLCRVLNA
                     AVEAGATTLNIPDTVGYTVPSEFGALIETIRQNVKGIEKVTISAHCHNDLGMAVANAL
                     AAVAAGARQVECTINGIGERAGNASLEEIVMAMRVRPDRYPYETGIVSEQIFAASQTL
                     SEITGVPVQPNKAITGRNAFAHEAGIHQDGVLKNPLTYEIMTPKSVGVPDSRLVLGKH
                     SGRHALGLRCEQLGYQFDRRALDDIYRRFVRLADKIKHVEDHHLLELIRDTHKPSTSA
                     TPLIEPIAAAQAAAVNASAASQASPNPAASEPVKPFPIAPPTNAFGVPLPAHGEAHHE
                     QTQQEDYLWGV"
     gene            3235..4152
                     /locus_tag="VEJ00_14400"
     CDS             3235..4152
                     /locus_tag="VEJ00_14400"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXX02403.1"
                     /translation="MELSQLEVFLAVARERRFSRAAEKLFRTQSAVSQTVRKLEDELG
                     EALFDRSSREGVLTDAGKVLYEYAEKLINLRSEATESLSELRELQKGKLVIAANEFTV
                     LYLLPVLAEFRRLHPMIKITVERALGSRIPDDVLRYSSEFGVLSYEPQEPSLHSVVVY
                     LDELVFVVPPKHPLASAPEVSIRQLGAESFVAHTVSSPYREKVIQLFKTHKTPLHMDM
                     ELPTLQSIKRCVAMGNGVAFVPEISVETELARGELVRIPVNELQLKRKLRLIYRKDAN
                     LSHAARAFLKVAEALAAERKGRYRFQREA"
     gene            complement(4210..4656)
                     /locus_tag="VEJ00_14405"
     CDS             complement(4210..4656)
                     /locus_tag="VEJ00_14405"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXX02404.1"
                     /translation="MQLENTEWQTWTQPPDVAAVELEKELKLKLSLEMRNRGDVIIVH
                     CQGRIVYRDEAAALSRVMGEVLRLGSNVILDLGGVDSMDSAGIGELVLLYTVARERNA
                     NLKWANPNGLVRTLLGLTNLDTVLDVHPSVDSALEALGEEQVCADC"
     gene            4854..5369
                     /locus_tag="VEJ00_14410"
     CDS             4854..5369
                     /locus_tag="VEJ00_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02405.1"
                     /translation="MSKTAAPLLALVGLMLLSMPMSAQILPRGNVYAGVSYGRLTDVI
                     PPRQSYRGFEGSAEDLPFARLPHLGFVLDGNGYYRSGVTEYNALLGPRLSATYGKWRP
                     FVHAMGGYQRLKSNGNVFTHIVIDFGGGADYRLWFKNFSWRLQGDYMHTHYLSSYQND
                     YRASTGLVWRF"
     gene            5458..6024
                     /locus_tag="VEJ00_14415"
     CDS             5458..6024
                     /locus_tag="VEJ00_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02406.1"
                     /translation="MRKVVHIAGVVFFFAGLAGAQVPTSGNVFFGYSYYNTDLSSSGR
                     ASTNGWEGTLEGKVFPFLGIVADISGDYGSQNFTTSVACGLPPCPPPITTFSANVSEY
                     NVLFGPRVSASIGKFRPFAEAEFGVGHVNASAAGSSTSFATALGGGLDYRLFRPVAWR
                     FQGDYVSTRFFSTTQNNVRLSTGIVLRF"
     gene            complement(6148..>6445)
                     /locus_tag="VEJ00_14420"
     CDS             complement(6148..>6445)
                     /locus_tag="VEJ00_14420"
                     /inference="COORDINATES: protein motif:HMM:NF024896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXX02407.1"
                     /translation="RPFATGMFMLFDRKKFWELGGFHEQILFAEDYRLSQKVERKRFA
                     IVRGGVYTTNRRFKRMGHLRVGWLFLKTAMNYWNEKYFLRDHKYWATEDSAPRA"
BASE COUNT         1254 a         1990 c         1847 g         1354 t
ORIGIN      
        1 cctggaggaa ggcgcagaca tcgccatcgg ctcgcgctgg ctgcgtgcgg aaacccagac
       61 gcagcgccaa ccgctgtacc ggcaggcctt tggccggatc tttaacctgc tgctccgcct
      121 cacgctgggc ttgaacttca aggacacgca gtgcggcttc aaggctttca aacaggctgg
      181 cgctcagtcg atcttcccat tgcagaagat cgaccgctgg ggctttgatc cggagattct
      241 ttttctggcg aggaagtgcg gctttcgggt tcgggaagtg gcagttgcct ggggacacag
      301 tggtggcacc cgcatcaatc ccctgaagga cggctcgcgt atgttcctgg agatgctgcg
      361 cgtccgctgg tacgactgga cgggaaaata tgcaggcaat ccaggtcctc gggctcaggc
      421 agggcaatcg ctccccacgg gccgcggacc gcgggcctga gccgtggctc aggttaccta
      481 tgctgacttc tctgctggcc gggaatcggg tttgctccgg acttgacggc ctctatcaca
      541 cactggccca tttctacggt cgagatcgca cgatgcgtct tgcccgctaa atctgcggtg
      601 cgcgcgcctt gcgaaagcac cgtcgatacc gctctctcga taccggctgc ctccgcctcc
      661 agatgaaacg aatggcgcag cagcatcgcc gcggacagaa tcgctcccag aggattggcg
      721 attcccttcc cggcgatgtc tggagcagaa ccgtgcaccg gctcgtagag cccaaccggg
      781 cctcctatgc tggccgaagc cagtaatcca agcgagccca cgacgccgcc cgcttgatcg
      841 gaaaggatgt cgccaaacat gttttccgtg agcacgacgt cgaagtccgt cgggcgctgc
      901 accagcagca tggcggccga gtccacatac tgatggccca gcttcacttc gggatagtct
      961 ttcccgaccc gcgtcacaac ctcccgccag agccgcgagc attcgagcac attggccttg
     1021 tccacggaga gcaccttgcg ccgccgcttc atcgcgagat cgaacgctac gtgcgcgatg
     1081 cgttctatct cggactcgcc ataacgcatc gtattaaccg ctacccgcga acccggcgag
     1141 ccctcgattg accgcggctg gccgaaatac gccccgccca gcagctcgcg cacgatcatg
     1201 atgtcggtgc cgcggacaag ttcggctcgc aggggagaac agtcttccag cgcgggaaag
     1261 aaaaccgccg gccgcagatt ggcgtacgcg cctaattcgc gccgcaatcg caaaagcccg
     1321 gcctcaggcc gaaggtggct cgggttgtgg tcgaacgctg gactgccgac agcaccgagc
     1381 agcaccgcgg cggacgagag gcaggcccga attgtgtctg cgggcagagg atcgttcgcc
     1441 acagcgagcg ccgctccgcc aatgggtttg cgctcaagct gaagttgatg accgaaggcg
     1501 tcagcgattg cctgcaaaac gagcacggcc tgttcggtga cttccgggcc gatgccgtcg
     1561 ccggggagga tggttaattt cagttgcata attccgtaat tttgtaatcg ggtaatttta
     1621 ttcggagacg aacaaaagcc gccagctttg gcggcttgat gattctcaat tgcaaactac
     1681 aaaattcccc gattacagaa tccctacacg ccccaaagat agtcttcctg ctgtgtctgc
     1741 tcgtggtgcg cttcaccgtg cgcaggcagc ggcacgccga aggcgttcgt cggcggcgcg
     1801 atcgggaacg gcttgaccgg ctcggatgcg gccggattcg gcgaagcctg gctcgccgcg
     1861 gacgcattca ccgccgccgc ttgtgcggcc gcaatcggtt cgatcaacgg cgtcgcggaa
     1921 gtggacggct tgtgcgtgtc gcgaatcagc tccagcaggt gatgatcttc cacgtgcttg
     1981 atcttgtccg ccagacgtac aaagcgccgg tagatatcgt ccagcgcgcg gcggtcgaac
     2041 tggtagccaa gctgttcgca gcgcagcccg agcgcgtgac ggccagaatg cttgcccagc
     2101 accagcctgg aatcgggcac gcccacggac ttcggcgtca tgatctcgta ggtcagcgga
     2161 ttcttcagca ctccgtcctg gtgaatgccg gcctcgtgcg cgaacgcatt gcgcccggtg
     2221 atggccttgt tcggctgcac cggaacacca gtaatctcgc tcagcgtctg gctggccgcg
     2281 aaaatctgct cgctcacgat gccggtctcg tagggatacc ggtcgggccg cactcgcatc
     2341 gccatgacga tctcttccag cgacgcgttg cccgcgcgct ccccaatgcc gttgattgtg
     2401 cactcgacct gccgggctcc ggcggccaca gcggccagag cgttggcgac cgccatgccc
     2461 aggtcgttgt ggcagtgcgc cgagatcgtt accttctcga ttcccttcac gttctgccgg
     2521 atggtctcga tcagggcgcc gaattccgag ggcacggtgt agccaaccgt atccggaata
     2581 ttcagcgtgg tcgctcccgc ctcaaccgct gcgttgagta cgcggcagag aaaatcgggc
     2641 tcggtgcgtg tggcatcttc cggggaaaac tcgacatcgg cgcacagcga cttcgcaaac
     2701 cggactgcgt cgcgcgtctg cgccacacac tggtcgcggg tgatgcgcaa cttgtactgc
     2761 aggtgaatgt cggaagtcgc cagaaatacg tgaatccggg gatgcgcggc gtccttcaat
     2821 gcctcccagg cacgctcgat atcctcccgg caggcgcgcg ccaacccggc gatcacaggt
     2881 cggcgcacta ccgcggcaat ggccttcacc gactcaaaat cgccttccga cgcgatggga
     2941 aacccggcct cgatgacatc cacgccaaga cggtcgagct ggtgcgccag ccgcagcttt
     3001 tcctgctggt tcatgctgca gcccggcgac tgctctccgt cgcgaagagt cgtatcgaat
     3061 accgtgacgc gtgctcgctc agtagccatc catctccgtt gacatcaagg cgtaatgctt
     3121 tatggtacgt aaccgcaccc ttgaagcaaa tctgataatt gtttagcaat gatcagtaat
     3181 attgctacaa taagtattgc taatggatta aggcactttg ttaaggaatg acagatggag
     3241 ctttcccaac tggaagtctt cctggcggtg gcccgcgagc gacgcttctc ccgcgccgcc
     3301 gaaaaactct tccgcacaca gtctgctgtc agccagaccg tccgcaagct agaggacgaa
     3361 cttggggaag cgctgtttga ccgctcttcc cgcgaaggcg tgcttaccga cgccggcaag
     3421 gtcctttacg aatacgccga aaagctcatc aacctccgca gtgaagcaac cgaatcgtta
     3481 tcggagctgc gggagctgca aaagggcaag ctggtcatcg cggccaacga gttcactgtg
     3541 ctgtatctcc tgccggtgct ggccgagttc cggcgcttgc atcccatgat caagatcact
     3601 gtggaacgcg cgctgggcag ccgcatcccg gatgacgtct tgcgctacag ctcggaattc
     3661 ggtgtcttgt cgtacgagcc ccaggaaccc agcctgcatt ctgtggtggt gtaccttgac
     3721 gaacttgtgt tcgtggttcc cccaaagcat ccgctggcgt ccgcacccga ggtgagcatc
     3781 cgccagctgg gcgcggagtc ctttgtcgcc cataccgtct cgtcccccta ccgggagaaa
     3841 gtgatccagt tgttcaaaac gcacaagacg ccgctgcaca tggatatgga gctgcccacc
     3901 ctgcagtcca tcaaacgctg cgtggctatg ggtaatggag tcgccttcgt gccggagatc
     3961 agtgtagaaa ccgagctggc gcggggagag ctggtccgga ttcctgtcaa cgagttgcaa
     4021 ctgaagcgca agctgcgcct gatttatcgc aaggatgcca acctgtcgca tgccgcgaga
     4081 gccttcttga aggttgccga agccctggcc gcggagcgta aaggccgtta tcgctttcag
     4141 cgcgaggcct gagcgaaaaa aaatgaggag cagaacgaat tctgctcctc aatggaatct
     4201 ctccttcggt cagcagtcgg cacacacctg ttcttcccct aaggcctcca gcgccgaatc
     4261 cactgacgga tgcacatcga gcacggtgtc gaggttcgtc aggccgagca gcgtccgaac
     4321 cagcccgttc gggttcgccc acttcaggtt cgcgttcctc tcacgagcca cggtgtagag
     4381 aaggaccagt tcgccgatgc ctgcgctgtc catcgaatca acgccaccca gatcgaggat
     4441 tacattgctc cccaaccgca gcacttcgcc catgacccgc gacaacgctg ctgcctcgtc
     4501 gcgataaacg atgcgacctt gacagtgcac gatgatcaca tcgccacggt tgcgcatttc
     4561 caggctcagc ttcaacttca actccttctc caactccacg gccgccacat ccggtggctg
     4621 cgtccacgtt tgccattctg tattctcaag ttgcatgcta tttaatagac gcttcagtgg
     4681 tggtttcggc accataaaaa tagagtgaat tttcgccgaa gggccatgcc cgtcgcgtcc
     4741 gaaacaaatt ttgtatcccg ggacatctgg ggcatccgtt acactgcatc taaccgattt
     4801 cattgagtag aatgattgtt tttgccggcg ctccggccgg ggaggaataa ttcgtgtcca
     4861 agactgctgc gccactgctg gcgctggttg gtttgatgtt gctctcgatg ccaatgtcgg
     4921 cacagatctt gccgcgcggg aatgtctacg ccggagtctc ctacggccgg ctcaccgacg
     4981 tcatccctcc ccggcagtcc tatcgcggct tcgaaggctc cgccgaagat ctccccttcg
     5041 cccgccttcc acatctcggc ttcgtgctcg acggcaacgg atactatcgc tccggcgtta
     5101 ccgaatataa cgcgctgctt ggaccacgcc tgtcggccac ctacggcaag tggcgtccct
     5161 tcgtccatgc catgggcggc taccagcggc tgaagtctaa tggaaacgtc ttcactcata
     5221 tcgtcatcga cttcggcggc ggcgcggact acagactctg gttcaaaaat ttctcctggc
     5281 gactgcaggg cgactacatg catacccact acctgagctc gtatcaaaac gattaccggg
     5341 cttcgacagg gctcgtctgg cgtttctgaa ggtttgctga tgtccgcacc cgcttccttg
     5401 cgcatctaag agctttctgg atggagtagg gccgattcgg accagaaagg ggccatcatg
     5461 cgcaaggtcg tccatattgc aggtgttgtt ttcttcttcg ccgggctcgc cggagctcag
     5521 gttccgacct caggcaatgt gttcttcggg tactcctact acaacaccga cttatcttcg
     5581 agcggtcggg ccagcacaaa tggttgggaa ggaacactgg agggcaaagt gtttccattt
     5641 cttggcattg tcgccgatat cagtggcgac tatggctcgc agaactttac aacgagcgtg
     5701 gcctgcggcc tgcctccttg cccaccgccc attacgacct tcagtgcgaa tgtctcggag
     5761 tacaacgttc tgttcggacc acgcgtgtcc gcatcgatcg ggaaattccg tccgtttgct
     5821 gaggcggagt tcggcgtagg gcacgtcaac gcaagtgcgg ccgggtccag cacttcgttc
     5881 gcgaccgcgt tgggcggcgg attggactac cggctgtttc gcccggttgc gtggcggttc
     5941 cagggtgatt atgtgtcgac gcgtttcttt agcaccacgc agaacaacgt gcgtctgtcg
     6001 accggtatcg ttctccggtt ctaaccgttg gccgagaaga attgcccgca aataaaagca
     6061 ggatccagct ggttggctga atcctgtttt ttgtggccac ggacaaaagt cgatttgaga
     6121 agggcacggc tcccgccgtg cggctggcta tgctctcggc gcgctatcct cagtcgccca
     6181 atacttgtga tcgcgcagga aatacttttc gttccagtag ttcatcgccg tcttcaggaa
     6241 cagccaaccc acgcgcagat gtcccatgcg tttgaagcgg cgattcgttg tataaactcc
     6301 gccccgcaca atcgcgaacc gcttgcgttc gactttttgg ctcaggcggt aatcttcggc
     6361 gaacaaaatc tgttcgtgaa atccgcccag ttcccagaac ttcttgcgat caaacagcat
     6421 gaacattccg gtagcgaacg ggcgg
//
LOCUS       DASPUL010000623         4645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_263355, whole genome shotgun sequence.
ACCESSION   DASPUL010000623 DASPUL010000000
VERSION     DASPUL010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263355"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..2333
                     /locus_tag="VEJ00_14425"
     CDS             282..2333
                     /locus_tag="VEJ00_14425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HXX02408.1"
                     /translation="MSTPVAELPVKKHKPYVPETMEMKEFTFRALLIGLVMTAILGSA
                     NAYLGLKAGMTIAATYPAAVIGMALLRLMKGSLLEENIARTVGSIGESVAAGAVFTIP
                     AFVMAGLWPGLTRDKYWNSVALMIIGGTLGILFVTLLRRVMVEDPELPYPESVAASEI
                     HKAGQQGAKAAKILFANMGFGGFMYLLGAVNVFSPNNTLHVKISEMGKRLLLRTGTNP
                     NVTTTVTGGATFFSMPDVSPAYLGVGYIIGPRLGALNFAGGVLAWGLLVPLLTFTVGP
                     YIQAAQPAGQTLSWDLLAGAIYYSIVRPIAVGGMLVGATYTLFKMRKQLAVGMGRAIS
                     DLRKSAAAHAATNRTERDLSSKVVFAGVALVFVAMIVLYYYFMSGAGNLTSSKIISGS
                     LVAAGVMVIVGFFFAAVSGNLVGMIGSSNNPVSGLTLCTLLIAALLMVALGVQGAGGV
                     AAVLGVAAVVCVSSAVAGEMLQDLKVGHILGGTPSKMEVGDLFGIVVASLVLFVPLAV
                     LDKAYHFGSNALPAPQAGLMAMLSKGIVGGDMAWPLVIVGILLGIVMILIEVKSVMLF
                     SVGMYLPLGTTFAIFVGGLIRWVTDRLRDRRGYNDAQKARVDNAGILTASGLIAGEAL
                     CGLVIAGLVGSGKDVTLVHWTPSIWTALVAMVVLLVAMVRVPLANAGSPDEPAPPTAI
                     M"
     gene            2423..3127
                     /locus_tag="VEJ00_14430"
     CDS             2423..3127
                     /locus_tag="VEJ00_14430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HXX02409.1"
                     /translation="MSIATNTAAIQERIAAAARRAGRSAGDIALMAVTKTHPPERIRE
                     AYAAGLRLFGENRVQEFATKAGAVADLAGAEWHMIGHLQTNKATKAAELFHAVDSVDT
                     VRLAEKLNASARALGKKLPVLIEVNVGGEAAKSGAAPGSPELEGLLLAAPRLEALDFR
                     GLMTVPPFTEDPEEARPFFRKLRELRNAISSRKLPRIDMIDLSMGMSHDFEVAVEEGS
                     TCVRVGTAIFGERKRV"
     gene            3240..3527
                     /locus_tag="VEJ00_14435"
     CDS             3240..3527
                     /locus_tag="VEJ00_14435"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HXX02410.1"
                     /translation="MPLIRESADGVTLAVRVQPRAKKNAITGEVGEALKVSLTAPPVE
                     GRANEACIEFFAKLLKLPRSSVTIASGHSSRKKVIRVARVTGQYVQDRLRR"
     gene            3600..>4645
                     /locus_tag="VEJ00_14440"
     CDS             3600..>4645
                     /locus_tag="VEJ00_14440"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX02411.1"
                     /translation="MTFSERLHEIRTGFERPFWVANISELFERLSYYAVFAVLARYLH
                     EGLQFDVERASSLTGMFGGWVWFLAVVGGAVADKLGFRRALSLAYLILSIAYFLLGSI
                     GATWFAPVRNAMPLVTLVAIVLVLPALGVALVKPCVVGTTAHSSSENVRSVGYSIYYT
                     LVNVGSTAGPLIASWAQKHGNSEYVFRIAALSVFIMFFLVLLLFKEPRRAAESEAATL
                     GQVGKNFLTVLSNPRFMLFLLIFTGYWIVFWQEFIALPLYISTYIDPKADTARILATD
                     PIVVICFTMVIGFLTKRLHAFHAIVLGTLISSLAWILLIVHPSVWMSVATLVVVAIGE
                     ITQQPRYYDYISRLA"
BASE COUNT          834 a         1365 c         1445 g         1001 t
ORIGIN      
        1 gcaagctcgg caatgggcgg acaccgctgc ccgcgccctt ctctgcccct gtcatcctga
       61 gcgtagcgaa ggacctccgc agcttgcggg gagtgcatgg gtccttcggc ctgcaaagag
      121 cgcaggcctc aggatgacaa atcggtgaag aatcgtgcgg tcgtacactt gtcacatgcc
      181 ctcgccgcgt gctaacgtgc gccgttcgtg agttttagct cggctgagaa ggcaacgcag
      241 gtgtaatagc ttccatccca tgctctaaaa ggattcattc aatgagcacc ccagtagcag
      301 aacttcccgt aaagaaacac aagccttacg tccccgaaac catggagatg aaggagttca
      361 ccttccgcgc cctgctgatc gggctggtga tgacggccat cctcggctcg gccaacgcgt
      421 atctggggtt gaaagcggga atgacgatcg ccgcaaccta tcccgcggca gtgatcggca
      481 tggcgttgct gcggctgatg aagggctcgc tgctcgaaga aaacatcgcg cgaaccgtgg
      541 gctcgatcgg cgagtcagtg gcggcgggcg cggtgttcac gattccagcg tttgtgatgg
      601 ctggcctgtg gccggggctc actcgtgaca agtactggaa ttctgtcgcg ctgatgatca
      661 tcggcggaac gctgggcatt ctgtttgtga ctcttttgcg ccgcgtcatg gtggaagatc
      721 cggaactgcc gtatcccgaa tctgtcgccg catctgaaat tcataaagct ggccagcagg
      781 gtgcgaaagc ggccaagatc ctgttcgcca atatgggttt cggtggattc atgtacctgc
      841 tgggtgcggt gaacgtgttc agcccgaaca acaccctgca cgtgaagatc agcgaaatgg
      901 gcaagaggct gctgctgcgt acaggtacga atcccaatgt cacgaccaca gtgaccggag
      961 gcgcaacgtt tttctccatg cccgacgtca gcccggcata tcttggcgtt ggctacatca
     1021 ttggcccccg gctcggcgca ctgaattttg ccggcggcgt gctcgcgtgg ggactgctgg
     1081 ttccccttct caccttcacg gtcggcccgt atatccaggc cgcgcaacct gcgggacaaa
     1141 cgctttcgtg ggacctgtta gcgggtgcaa tctactactc catcgtgcgc cccatcgcgg
     1201 tcgggggaat gctggtgggc gcgacgtaca ccctattcaa gatgcgcaag cagctcgccg
     1261 tcggtatggg gcgagcgatc tcagacttgc ggaaatcggc ggccgctcac gccgccacca
     1321 accgtaccga gcgcgatctg agctcgaagg tcgtatttgc cggcgttgcc ctggttttcg
     1381 tggccatgat cgttctctac tactacttta tgagtggggc cggaaacctg acgagttcga
     1441 aaatcatctc gggatcgctt gtcgccgccg gcgtgatggt gatcgtcgga tttttcttcg
     1501 ccgccgtatc gggaaatctc gtgggaatga ttgggtcgtc gaacaatcca gtgtcgggac
     1561 tcaccctctg cacgctgctg atcgccgcct tgttaatggt tgctcttgga gttcagggcg
     1621 ccggaggagt agcggcggtg ctaggagtag cggcggtggt ctgcgtctcg tcagccgttg
     1681 cgggggagat gctgcaggac ctgaaggtcg gtcacattct tggcggcacg ccctccaaaa
     1741 tggaagtcgg cgatctgttc ggcatcgttg tggcgtcgct ggtattgttt gtcccgctcg
     1801 ccgtactgga caaggcttac cactttggca gcaacgccct gcccgcgccg caggctgggc
     1861 tgatggcgat gctctccaag ggcatcgtcg gtggcgacat ggcatggcct ctggtgatcg
     1921 tgggcattct gctcggcatc gtcatgatcc tgattgaagt caagagcgtg atgctgttct
     1981 ccgtgggcat gtacctgccg ctgggaacga cgttcgccat ttttgtaggc ggcctgatcc
     2041 gctgggtgac agacagactc cgcgatcgcc gcggctacaa cgacgcgcag aaggcgcgtg
     2101 tggacaatgc cggcatcctg accgcctcgg gactcatcgc cggcgaagcg ctgtgcgggc
     2161 tcgtgattgc cggactcgtc ggttcgggaa aagacgtgac tctcgttcat tggacgcctt
     2221 cgatctggac cgcgctggtg gccatggtcg tcctgctggt ggccatggtc cgcgttcccc
     2281 tggccaatgc gggcagccct gacgagcccg cgccgccgac ggcgatcatg tgagcgagcg
     2341 aagcgggagc gcggcgtcat cccgagcgaa gtcgagggat ctctgagcgt caacgtcgca
     2401 ctcctcctgt catcgtgctc tcatgtccat tgccaccaac accgctgcga ttcaggagcg
     2461 catcgcggcc gcggcgcgcc gggctggacg ctcagcgggt gacatcgcgc tgatggcggt
     2521 gaccaaaact catccgccgg agcgtattcg cgaagcctac gccgccggcc tgcggctgtt
     2581 tggcgagaat cgcgtgcagg agttcgccac caaggccggc gcagtcgccg atcttgccgg
     2641 cgccgagtgg cacatgatcg ggcatttgca aaccaacaaa gccaccaagg cggcagagtt
     2701 attccacgcg gtggactccg tcgacaccgt caggctggca gagaaactca acgcgtcagc
     2761 gcgagccctg ggcaagaaac tgccggtgct gatcgaggtg aacgtcggcg gagaagccgc
     2821 caagagcggg gctgcgcccg gctcgccgga gttggaaggt ttgctgctcg ccgcgccccg
     2881 gctggaggct ctagactttc gcggcctgat gaccgtgcct ccgttcaccg aggatccgga
     2941 agaggcgcgg ccgtttttcc gcaaacttcg cgaattgcgg aatgctatca gctcgcggaa
     3001 gttgccccgt atcgacatga tcgacctctc catgggcatg tcgcacgact tcgaagtcgc
     3061 ggtcgaggaa ggatcgacct gcgtgcgcgt gggcaccgca atctttgggg aaaggaaacg
     3121 agtctaacca cggaatcaca gaggcacagg gaagatcttg aactttcttc cggcccaaag
     3181 tgcccgcgta atgcgagctg tcgatcttct ctgtgtctcc tgcctccgtt ggtgaactta
     3241 tgcctctgat tcgcgagagt gctgacggcg tgacgctcgc tgtcagagtg cagccgcgcg
     3301 caaagaagaa tgccatcacg ggcgaggtcg gcgaggcgct gaaagtttcc ctcactgccc
     3361 caccagtcga aggccgagcc aacgaggcgt gcatcgaatt ctttgccaag cttttgaagc
     3421 tgccgcgaag ttccgttacc attgcctccg gccacagtag ccgaaaaaaa gtgattcgcg
     3481 ttgcccgcgt gacggggcag tacgttcagg accggctgcg caggtagttg tcgacttcat
     3541 agctggcgtc ggcagttcgc ggacaggagt gtccgcgcca cacaagcaca ggagtcacat
     3601 tgaccttctc cgaacgtctc cacgaaatcc ggaccgggtt cgagcggccc ttctgggtcg
     3661 cgaacatcag cgaactgttt gagcggctct cgtactacgc cgtgtttgcg gtgctggccc
     3721 gctacctgca tgagggcttg cagttcgatg tggaacgagc cagcagtctg acgggaatgt
     3781 tcggcggctg ggtgtggttt ctcgccgtgg tgggtggggc ggttgcagac aaactcggct
     3841 tccggcgcgc tctttcgctg gcctacctga ttctgagcat cgcatatttt ttgttgggct
     3901 cgatcggcgc aacctggttc gcgcccgtgc ggaatgccat gccgcttgta acacttgtcg
     3961 ccatcgtgct ggtcctcccg gcgctgggtg tggccctggt gaagccgtgc gtcgtgggca
     4021 ctacggcaca ttcttccagc gaaaatgtgc gctcggttgg gtactcgatc tactacacgc
     4081 tcgtgaatgt cggcagcacg gcgggaccgc tcatcgcctc ctgggcgcag aagcatggca
     4141 actcggagta tgtgtttcgc attgcggcgc tgagcgtgtt tatcatgttc ttcctggtgc
     4201 tactgctgtt caaagagccg cgccgcgcgg ccgagtccga agcggcaact ctcggtcagg
     4261 tgggaaagaa cttcctgacc gtgctctcaa acccgcgctt catgcttttt ctgttgattt
     4321 ttaccggata ctggatcgtc ttctggcagg aattcattgc gctgcctttg tacatctcca
     4381 cctatatcga cccgaaggca gatacagcgc ggattctggc caccgatccg atcgtcgtca
     4441 tctgtttcac catggtcatc ggcttcctga ccaaacgcct gcacgcgttc cacgccatag
     4501 tgctgggaac actgatttca agcctggcct ggatcttgct gattgtgcac ccgtcggttt
     4561 ggatgtcagt ggcgacgctg gtcgtcgttg ccataggaga gatcacccag caaccgcgtt
     4621 actacgacta catttctcgc ctcgc
//
LOCUS       DASPUL010000624         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1620986, whole genome shotgun sequence.
ACCESSION   DASPUL010000624 DASPUL010000000
VERSION     DASPUL010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620986"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VEJ00_14445"
     CDS             complement(<1..695)
                     /locus_tag="VEJ00_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXX02412.1"
                     /translation="MTETKRPTINWMDALSLLILAGLALLPPVQEPHKQLILLAFGVL
                     QVAESWVLARMPRRGSAYVVLLKIALATLLIDHTGEISINSSYWPIYFLPAVTAAEYF
                     GPVATLLWTTLASAAYCSYLYQASLEFELTEEAYGLLAIRVLFFFLAAMVVNRYVVES
                     RKQTQRYQDLAETLAETNRRLEQAQAEARRSERLAALGQLSAGLAHEIRNPLGVIKGS
                     AEMLNQKLGKADPL"
     gene            complement(860..>2104)
                     /locus_tag="VEJ00_14450"
     CDS             complement(860..>2104)
                     /locus_tag="VEJ00_14450"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HXX02413.1"
                     /translation="EVWQPTAVAVGWAPYRFGHWIWVSPWGWTWVEDEPWGFAPFHYG
                     RWAFVGARWFWVPGPVVVRPVWAPALVAFVGGGPGFHFSAGVGVGWFPLGPGEVFVPG
                     YHVSRGYVNNVNITNTTVNVTKVTNVYNTVIVNKTTTINNITYVNQHVNNAVTVVSHD
                     AFVNSRPVAQNVMKVEPRELAAAPVSHVIAAEPLRTSVIGAGRPVAVKPPAPVINRQV
                     VAVRTPPPLPRSLDQRQTEAGGHLNQQLLVRPAGPARPVQMTPATQQPATQGGFKSFG
                     QPSQPSGGNTQVKQMPQTQPRVYEQQGTPQPEPRPENRTVEEQTNRNVQPPTRNVQPP
                     SRNVQPESENRSFQPPPQEMTHPLVHPAPPVEEHNPQVEQQKEQKFQQWQQQRPPASQ
                     PRSQPQPRSEPPKQSAPPKKGR"
BASE COUNT          366 a          652 c          688 g          398 t
ORIGIN      
        1 aggggatcgg ccttccccag cttctggttc agcatttccg ccgagccttt gatcacgccc
       61 agcggattgc gaatctcgtg cgccaggccg gcagagagct ggcccagggc ggccagacgc
      121 tccgagcgcc gggcttcggc ctgcgcctgc tcaaggcggc gattggtttc ggccagggtc
      181 tcggccaggt cctgatagcg ctgggtctgc ttccggcttt cgaccacgta gcggttcacc
      241 accatggcgg cgagaaagaa gaagagaaca cgaatcgcca gaaggccgta cgcttcctcg
      301 gtcagttcga attccagcga cgcctgataa agatatgagc aataggcggc ggaggcgaga
      361 gtggtccaga gcagcgtcgc caccgggccg aaatattcgg ccgcagtaac tgcaggtaga
      421 aagtagatcg gccagtagct gctgttgatg ctgatctcgc cagtatggtc gatcagcaga
      481 gtcgccaagg caatcttcag cagcacgacg tacgcggagc cacggcgggg catacgggcc
      541 aacacccagc tttcggccac ctggagcaca ccaaaggcca gcaggatgag ctgcttgtgc
      601 ggctcttgca ccggcggaag cagggccaag cccgccagaa tcaggagcga cagagcatcc
      661 atccagttga ttgtgggtcg cttggtctcg gtcatcgctc tgcctgttgg atgatgtaaa
      721 agggcgcctt gcgcaaaaag ttacgccggg agaggttgcg ccgctcccgg cgtgatcgtt
      781 atggcttatc gggtccgcaa gagcatctcc agcgaacctt atcacagtgc acaatcacag
      841 cgcacaacca cagccagact cagcgtcctt tcttgggcgg ggcgctttgc ttgggcggtt
      901 ccgaccttgg ctgcggctgc gacctgggct gtgaggccgg gggccgctgc tgctgccact
      961 gctggaactt ctgctccttc tgctgctcca cctgcgggtt gtgctcttcg accggaggtg
     1021 ccggatgcac cagcggatgc gtcatttcct ggggcggagg ctggaacgag cggttctcgc
     1081 tctcgggctg aacgttccgg ctcgggggct gcacgttccg ggttgggggc tgtacgttgc
     1141 gattcgtctg ctcctccaca gtccgattct cgggccgggg ttcgggctgt ggagtcccct
     1201 gctgttcgta cacgcgcggc tgggtctgcg gcatctgctt gacctgagtg tttccgccgc
     1261 tcggctgact gggttgcccg aaggacttga atcctccttg tgtggccggc tgttgtgtcg
     1321 ccggagtcat ctggacgggg cgagcaggac ctgccggacg caccagcaac tgctgattca
     1381 ggtgcccgcc ggcctccgtc tgccgctggt cgagcgaacg gggcagcgga ggcggggttc
     1441 tgaccgcgac tacctgacgg ttgatcacgg gggccggcgg cttgaccgct accgggcgtc
     1501 ccgcaccaat aacgctggtg cgcagcggtt cggcggcaat gacgtgactg accggcgccg
     1561 cggcgagctc gcgcggctca accttcatca cgttctgcgc gacgggccgc gagtttacaa
     1621 aggcgtcatg cgataccaca gtcacggcgt tgttcacgtg ctggttcacg taggtgatgt
     1681 tgttgatggt cgtggtcttg ttgacgatga ccgtgttgta cacgttcgtg accttggtga
     1741 cgttcaccgt ggtattggtg atgttcacgt tgttcacgta accgcggctc acgtggtatc
     1801 cggggacaaa cacttctccg ggccccagcg ggaaccatcc gacgccaacg cccgcggaga
     1861 agtggaagcc cgggccaccg ccgacgaaag ctaccagagc gggcgcccac accgggcgga
     1921 cgaccacagg tcccggcacc cagaaccacc gtgcgccgac aaacgcccag cgtccgtagt
     1981 ggaagggagc aaagccccac ggctcatcct cgacccacgt ccagccccat gggctgaccc
     2041 agatccagtg accgaagcga tagggcgccc accccaccgc aactgcggtc ggctgccaga
     2101 cttc
//
LOCUS       DASPUL010000625         5264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_120293, whole genome shotgun sequence.
ACCESSION   DASPUL010000625 DASPUL010000000
VERSION     DASPUL010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120293"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VEJ00_14455"
     CDS             <1..295
                     /locus_tag="VEJ00_14455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXX02414.1"
                     /translation="DVVCRYGGEEFAILLTQTSAPQAMIVAEKLRRMVEGWQFPGVAR
                     TVTVSAGISAFPTHGTNRDGLVRAADTALYAAKQAGRNQVRLAVATRAAAHGS"
     gene            complement(304..1428)
                     /locus_tag="VEJ00_14460"
     CDS             complement(304..1428)
                     /locus_tag="VEJ00_14460"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXX02415.1"
                     /translation="MRKYILPSIALLPLLLTSSYAAQTWSVDPPARAWVFSSEEGGTG
                     SYLGVDITDITPDRMSALKLKEEKGVEVTMVDQDAPAGKAGMKEHDVILTMNGSSVES
                     GAQLRRMIHETPPGRVVTFGVSRDGQPLTIKLQLADRQKEFAMGGPKAKEFHFEMPKM
                     PEMDFMPSINMVVVTSSVRSGLTVENITPQLGEFFGVKNGNGVLVRSVEKGSRAEKAG
                     FHAGDVIVKVNDQFVHDTSDFTHAVKSRTGDSVSVSVVRDKKEQNLNLELPAHKDSSD
                     LYDESSDSTVIDADAQIELGELREEMAKLRPQFELAAENARKAAEEVRKSFCDQQRQI
                     REQAQKQAEQQRRWFKDNQDRIRQQLERMRQELSGNWLDI"
     gene            complement(1588..2796)
                     /locus_tag="VEJ00_14465"
     CDS             complement(1588..2796)
                     /locus_tag="VEJ00_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02416.1"
                     /translation="MRRSPQIVGLAGLLALLAPLVFPSKSLAQESHITREGGAWGQEI
                     TGSLAGVKTLRVKVDMGSVVVHGGQQQAINYVIHTRFGNSNEQDARRQFEQYKITAYV
                     KGDTAWIVGDWQGGRQPRHCSSEFSVMVPREMALVKLETEGGNVDATGVEGKVEAESA
                     GGSMHLDDIGGAYAETGGGSIEVGTVHGDLGLHTGGGSIEVHHADGKITAETGGGSVE
                     IHSGSQGAVIETGGGSIELRQCNGKAKVSTGGGSVDLSDIAGPADIETGGGSIHLTSA
                     KGHVVAETGGGGIELYGVPSARAETGAGGVVVKLVNTGGERRDSDLETGAGDIMVYIA
                     SDVAVNVRASVDIANGHHITSDFPDIHISSEGGPWGPKTYTAEGKLNGGGPMLKVHTS
                     TGDIVFKRAQ"
     gene            complement(2919..3857)
                     /locus_tag="VEJ00_14470"
     CDS             complement(2919..3857)
                     /locus_tag="VEJ00_14470"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXX02417.1"
                     /translation="MKCEWVRANIVLQVYGELADDARHELEQHVARCADCAAELKAEQ
                     DFHALLSQVRAAEPSPNLLAASRMRLQEALETAKQGSFWHRLAFDPANWLRQMRFSPA
                     LASAILILGFAGGVGTTYRIYGSRTVDKPIIDRPTSAPTEASIAAIDSVTPVPGTDQV
                     AIKYNMVTTQQTQGSLNDQQIQQLLLYAARSNYNSGVRVDSVDLLAKRSSDQQVREAL
                     MYALQNDTNPGVRLKSLDALGNYVRDDTDVRDAVLRALVSDTNSGVRIEALRLIEPVK
                     ADGSVRGVLMALAAKDQSTYIKSQARTMLAQLPEID"
     gene            complement(3948..4553)
                     /locus_tag="VEJ00_14475"
     CDS             complement(3948..4553)
                     /locus_tag="VEJ00_14475"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXX02418.1"
                     /translation="MAVPDRIQDRRIDDTMLIREAQRGNAAAFEELVRHYDKSVLRVA
                     LNLTGTEQDAQDIYQEAFLKAYKNIGNFRFECSFYTWVYRIVTNLCLDHLRKRNVRKE
                     DAPVAKDADGGEYDLLDQVADGRAGANPERDLMRRQLGSRIAGALDKLTPRERMVFEL
                     KHYHGLKLRTVGEILHTTEETAKNTLFRATQKLRGALADMR"
BASE COUNT          902 a         1630 c         1590 g         1142 t
ORIGIN      
        1 tgacgtggtg tgccgctacg gcggagaaga attcgcgatc ctgctaaccc agacgagcgc
       61 gcctcaagcc atgatcgtgg cggaaaagct gcgaaggatg gtggaaggat ggcaatttcc
      121 gggagtagcg cgcacggtga ccgtcagcgc cggaatttca gcctttccca cgcacggcac
      181 gaaccgcgat ggtttggtgc gagccgccga caccgcactc tatgctgcca agcaagctgg
      241 acggaaccag gttcgtctgg ctgtggcgac ccgcgcggct gcgcatggca gctagccccg
      301 aggctagatg tcgagccagt tgccgctcag ttcctggcgc atccgttcca gttgctgtct
      361 gatccggtcc tggttgtcct tgaaccatct ccgctgttgc tcggcctgtt tttgggcctg
      421 ctcgcggatc tggcgttgct gatcgcagaa agatttcctg acctcttccg cggccttgcg
      481 cgcattttcg gccgcgagtt cgaactgcgg ccgtagtttt gccatctctt cccgtaattc
      541 gccgagctcg atttgcgcat cggcgtcaat caccgtgctg tccgaactct cgtcataaag
      601 atcgctggaa tccttgtgcg cgggcaactc cagattcagg ttttgttctt tcttgtcgcg
      661 gaccacgctg acgctcaccg aatcgccggt gcgggacttg accgcatggg tgaagtcgct
      721 ggtgtcgtgc acgaactgat cgttgacttt cacgatgacg tccccggcgt ggaagcccgc
      781 tttttcggcc cggcttccct tttccaccga gcgcaccagc acgccattgc cgtttttcac
      841 tccgaagaat tccccgagtt ggggagtgat gttttccacc gtcaggccgc ttcgcaccga
      901 agaagtgacg accaccatgt tgatggaagg catgaaatcc atctcaggca ttttgggcat
      961 ttcgaagtga aattccttcg ccttagggcc gcccatggcg aattctttct ggcggtcggc
     1021 gagttgcagc ttgatggtca ggggctggcc gtcgcggcta acgccaaagg tgaccacgcg
     1081 gccgggcggg gtttcgtgga tcatgcggcg cagctgcgcg ccgctttcga cggagctacc
     1141 gttcatggtg aggatgacgt cgtgctcctt catgccggct ttgcctgcgg gagcgtcctg
     1201 atcgaccatc gtgacctcga cgcctttttc ctctttcagc ttcagcgcgc tcatgcgatc
     1261 cggggttatg tcggtgatgt ccacgcccag gtacgagccg gtaccgcctt cttcgctcga
     1321 gaacacccag gcgcgcgccg gcgggtctac cgaccaggtc tgcgccgcat aggaagatgt
     1381 gagcagcaac ggcagcaaag cgatgcttgg caggatgtat ttgcgcatgg cagctcctta
     1441 caacttgaac ttactcaggc gccgggcaga atcgatgatt cttcccggtc tccggcaatg
     1501 tttgatcgat ggtttttgat tcttgaacaa aagccaattg tttctgagct tcattcaaca
     1561 atcgtcagtc atcaatccac gatttcgtta ctgtgctcgc ttgaagacga tgtcgcctgt
     1621 cgacgtgtgc accttcagca ttggaccccc gccattcaac ttgccttcgg cggtgtaggt
     1681 tttcggcccc cacgggccgc cctcgctgct gatgtgaatg tcagggaagt cggacgtgat
     1741 gtggtgcccg ttggcgatat cgacgctggc gcgcacgttg accgcaacgt cggacgcgat
     1801 gtagaccatg atgtctcccg cgccggtttc gaggtcggaa tcgcggcgct cgccgccggt
     1861 gttgaccagc ttgaccacca ctcctccagc tccggtctcc gcgcgggccg agggcactcc
     1921 gtaaagctcg atgccgccgc cgccagtttc cgccaccacg tgccctttgg ctgacgtcag
     1981 gtgaatgctg ccgccgcccg tttcaatgtc ggcaggaccc gcgatgtcgc tcagatcgac
     2041 gctgccgccg ccggtggaaa ccttcgcctt gccattgcac tggcggagct cgatgctgcc
     2101 gcctccggtc tcgatgactg cgccctgcga tccggagtga atctcgacac ttccgccgcc
     2161 ggtttcggct gtgatcttgc cgtcggcgtg atgcacttca atcgagccac cgccggtgtg
     2221 caggcccaaa tcgccgtgga cggtgccgac ctcgatcgat ccgccgccgg tttcggcgta
     2281 ggcgccgccg atgtcgtcca gatgcatgct gccgccggcg ctctcggctt ccaccttgcc
     2341 ttcaactccg gtggcatcca cgttgccgcc ctcggtttcg agcttcacca gcgccatttc
     2401 ccgcggaacc atcaccgaaa actcgctgga gcaatgacgc ggctgccttc cgccctgcca
     2461 atcgcccacg atccaggcgg tgtcgccctt cacataagcg gtgattttgt actgctcgaa
     2521 ctggcggcgg gcgtcctgct cgttagaatt gccaaaacga gtatgaatga cgtaattgat
     2581 ggcttgctgt tgcccgccat gcaccacgac cgaacccata tcaaccttca cccgcagagt
     2641 ctttaccccg gccagcgaac ctgtgatctc ctgaccccag gctccgcctt cgcgggtgat
     2701 gtgtgattcc tgcgccaggc tcttcgaagg gaagaccagc ggcgcaagca gggccagcag
     2761 accggccagc ccaacgattt gtggcgaacg tctcaaatct ctacctccaa tctcgcggag
     2821 tggccgcgaa cctaaggggc taaagccccg tgtcatactc ctgaactctt tcttcccggc
     2881 gcggctggag ccgtgccctt tccaattcgt gcgccggtct aatcaatctc gggcaactgg
     2941 gccagcatgg tgcgggcctg cgacttgatg tacgtgctct gatcttttgc ggccagcgcc
     3001 atgagcacgc cgcgcacgct gccgtcggct ttcaccggct cgatcaggcg cagggcttcg
     3061 atgcgcactc cagaatttgt gtcgctgacc aaagcacgca gaacggcgtc gcgcacatcg
     3121 gtatcgtcgc ggacgtaatt gcccagcgcg tccagtgact tcagccggac cccgggattc
     3181 gtgtcgttct gcagggcata catcagggct tcccgcacct gctggtcact gcttcttttg
     3241 gccagcagat ccacggaatc tacgcgcacg ccggagttgt agttgctgcg ggcggcgtag
     3301 agcaggagct gctggatctg ctggtcgttc agcgatccct gcgtctgctg cgttgtcacc
     3361 atgttgtatt tgattgcgac ctggtcggtg ccggggacgg gagttacgga atcgatggcg
     3421 gcgatggaag cttcggtagg agcgcttgtc gggcgatcaa tgatgggctt gtcgacggtg
     3481 cgcgatccat aaatcctgta ggttgttccc accccgcccg cgaagcccag gatcagaatg
     3541 gccgaagcca gcgccgggga gaaacgcatc tgacgcagcc agttcgccgg atcgaaggcc
     3601 agccggtgcc agaagctgcc ctgcttcgcg gtctccagcg cttcctgcag acgcatcctc
     3661 gaggcggcca gcaggttggg cgacggctca gcggcgcgaa cctgcgagag cagggcgtgg
     3721 aagtcctgct cggccttcag ttcggcagcg caatcggcgc agcgggccac gtgctgctcc
     3781 agttcgtggc gggcgtcgtc agccagctcg ccgtaaacct ggagcacgat atttgctctt
     3841 acccattcgc acttcataag cttcttacct cagttacgtt cttgctcgtg cggccgcgct
     3901 tcgcgctggc cggacaggcg aggcgcccgt ccccacacgg tccttgccta tcgcatgtcc
     3961 gccagagccc cgcgcaactt ttgcgtggcg cgaaacaacg tgttcttggc ggtctcttcc
     4021 gtcgtatgca aaatctcgcc cacggtgcgc agcttcaatc cgtggtagtg cttcagctcg
     4081 aacaccatgc gctcgcgcgg agtgagcttg tcgagcgctc cggcgatgcg cgatcccaac
     4141 tgccggcgca tcaagtcgcg ctccggattc gccccggccc ggccgtcggc cacctgatcg
     4201 agcagatcgt actcgccgcc gtcggcgtct ttcgccaccg gagcgtcttc cttgcgcaca
     4261 ttcctcttgc gcagatgatc gagacacagg ttggtcacga tccggtaaac ccaggtgtag
     4321 aaggagcact cgaaccggaa atttccgatg ttcttgtagg ccttgagaaa cgcttcctga
     4381 tagatatcct gcgcgtcctg ctcggttccg gtcagattca gagcgacacg caacaccgac
     4441 ttgtcatagt gacgaaccag ttcctcgaag gcggcggcat tgccgcgctg cgcctcgcgg
     4501 atgagcatcg tgtcatcgat gcggcggtct tgtatgcgat ctggaacagc catgcggctc
     4561 cgcgcggggt actgattgtc acgggctatc gaaccgctga ctctctctgt ctgctcactc
     4621 ttgatggagt gggcccgtcc gccaaccgca agcgaaaccg cccaagcatc ggccggcatt
     4681 tttgtcttgt cctctacctg cccggcttct ttgccttagt aagctatgcc gttccgtccg
     4741 ggcttgcaca ggaatagacg aggggtcaga agaaccgtga gcagaaatta tagagcctgg
     4801 gacgaatttt tgcggggcta ggtgattctg ggacaggggg ttaggggaga acagaacatg
     4861 gttgcacagg agccggggga gggtttggca ggggaagtgc cctgaaggtg gggcagtcag
     4921 acgcagcctc gcagccgacg caagacccga atgcgcttgg agagccgcag atctggccca
     4981 tgaagctggt ttcaggtgcc gccccggagg ggcacttgat ggtagcctgc cacttcagtg
     5041 gcgggtcacg ttgcatttga tgacgtccgt gccggaggca cggctgaaaa gccattggcg
     5101 aagttttcaa gcgtccctac tgggcgcgcg aagggtttgg ggtgagcgca tacccgccag
     5161 tgaactggcg ggctattttc atcggtgcct acggcaccgg cgtcgtcgag cgcgaccgcg
     5221 agcaggatgt cagaggtaga cgctgggtgt atgccgcccc tgga
//
LOCUS       DASPUL010000626         8444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1217894, whole genome shotgun sequence.
ACCESSION   DASPUL010000626 DASPUL010000000
VERSION     DASPUL010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8444)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217894"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VEJ00_14480"
     CDS             <1..943
                     /locus_tag="VEJ00_14480"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXX02419.1"
                     /translation="ETLDNALYDAFGQRQVSTMYRPLNQYHVVMEVAPQFQQTPESLQ
                     NIYLRSTAGSPVPLSAFTHFEPSNTPLAVNHQAQLPSATISFNLAPGVSLGQATDAIE
                     QAERTIGFPPTIQASFQGTAAAFQDSKSNMLILIIAALGAVYIVLGMLYESYVHPITI
                     LSTIPSAGVGALLALLLTGNELNVIGMIGIILLIGLVKKNAIMMIDVALDVERTQGKK
                     PVEAIYDACILRFRPIMMTTMAALLGGLPLALGRGTGSELHRPLGITIVGGLIVSQAL
                     TLFTTPVVYVYLDRLRLALRGGVDRALPDAGAPSPAH"
     gene            1281..2363
                     /locus_tag="VEJ00_14485"
     CDS             1281..2363
                     /locus_tag="VEJ00_14485"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HXX02420.1"
                     /translation="MLKKAAALLVVCVSVATWVSCGTTVSHFVYASTPASNQIAAYRE
                     DPNSGVLTALAGSPFEAGNGVEAIALHPTKKFLYAVNSFSSDISLFTVSTLGAITEMM
                     PRTPVTPGGTNPKFLAIDTAGSFLYVGNIGSNNISVFSIDASSGDLKEISGSPFPIGM
                     TPLNMALAPNGNVLYVTGGGSGQQGIIQIFTLTAGVLSPIAGPAVPPGRSPVGLVINS
                     GGSILYTANRLDNTISGYSIATDGSLSLISGFPLSVSGLSGPDAMLIDNSGKYLYVAN
                     GLSNNLTALSISSGGALTLLGNSPFGTASQPNFIASDPSGKHLFVGNQVGPAIESFRL
                     DTGTGTLTSVASYSVGNVPSSIVVGQ"
     gene            2573..3622
                     /locus_tag="VEJ00_14490"
     CDS             2573..3622
                     /locus_tag="VEJ00_14490"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HXX02421.1"
                     /translation="MRISSAASAFPKHYYSQKFLLQKLQEYWGDSLRNPQMLARLHRN
                     VAVEGRHLAMPAEKYYEMTTWGRANDIWIEVGQELGEQALCRALHHAGLEPSQLGALF
                     FTSVTGISSPSIDALLINRMGLPANIRRVPIFGLGCVAGAAGIARAADYVRAYPSQAA
                     ALVSVELCSLTIQRDDLSVANLISSGLFADGSAAVIVTGDELDSTGPQILATRSIFYP
                     DTEEMMGWNISEKGFRITLSPQVPVLIREHLGHDVDTFLADQGYQRSDIGSWVLHTGG
                     PKVLEATAAALELNNGQLDASWDCLRKVGNLSSASVLVVLEDVMRNRRPQPGTLGLLA
                     AMGPGFCSELVLLQW"
     gene            3632..4753
                     /locus_tag="VEJ00_14495"
     CDS             3632..4753
                     /locus_tag="VEJ00_14495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXX02422.1"
                     /translation="MSQRVPATTDVFVIGGGPAGLAAAITARQRGLSVVVADGAQPPV
                     DKACGEGLLPDALAALERLGIHLSATYAHPFGGIRFVGSTLSADAPFPAKSYGLSLRR
                     TALHRVMMERASQLGAVLLWQTAVTGISSDGVHTGKGLVSARWIVGADGCNSRVRHWA
                     GLDRGSRFRYRYAFRRHYRVAPWSDFMEVYWGDRCQGYATAVSGDQVCVALASHDPDF
                     RLEDGLRALPRLGARLAGAEPASAERGALTGNRTLRQVRRNNVVLIGDASGMVDAITG
                     EGLGLAFRQAVVLAECLASGDMARYQALHWRFALRPLWMARLMLTLDGRPRLQHRTLQ
                     VFQKRPEVFQHLLELHVGAASPLDVALDGLTLGWGLLTA"
     gene            4816..5379
                     /locus_tag="VEJ00_14500"
     CDS             4816..5379
                     /locus_tag="VEJ00_14500"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXX02423.1"
                     /translation="MVAIAPATAQQASLTIDPVKTTLSFTLGAALHTVHGSFQVKPGT
                     LELNPVSGQVSGEIDVDAKSGETGSGMRDRKMHKDVLESQRYPEISFRPDRVEGAVAS
                     LGRSTVKVHGMFRIHGVDREITVPAVVEMAGDHWSATVHFTVPYAKWGMQNPSTLFLR
                     VSDSVEIELAVGGSAAQHGAAAASSDQ"
     gene            complement(5373..7076)
                     /locus_tag="VEJ00_14505"
     CDS             complement(5373..7076)
                     /locus_tag="VEJ00_14505"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HXX02424.1"
                     /translation="MNRRSFLWGMGAVAASPYWPRLARPAAQTGAGASPGFRFADVTS
                     QAGVEFQHNSGAFGGKFLPETLGSGCAFLDYDRDGWQDILLINGADWPGHKKKRTTLR
                     LYRNNGNGTFTDVTAHAGLDIELYGMGVAVGDYNNDGFPDILVTCVGQSRLFRNTGKG
                     TFIDVTNSSGLGKREGFSTSALWFDYDRDGLLDLFVCNYVKWSPEQDVFCSLDGKHKS
                     YCTPEAYRGATCWLFHNRGDGTFEDVTATSGIFDSSSKSLGVALLDDNRDGWPDLLVA
                     NDTQPNKLYRNQRNGSFKDVAVEAGLAFSSEGKARAGMGVDVADFENSGTPGVAITNF
                     DNEMIGLYRANSKSFEDIAPQSGVGMASRNSLGFGCVFLDANLDGWLDFAVANGHIDE
                     TVRNIRGNVGYAQPPQLFLNGGKDTFRDIATEVGGGFDQPKVGRGLAYGDFDRDGDLD
                     LLLTTNNGPAYLYRNDVLNGNRSIRFRLVGTKSNRDAIGATVHITGGGVTQSRMVKGG
                     SSYLSQSELPVTFGLEKRDRIERVVIDWPSGRTEEYKDVAAGRCYEVTEGKGITPQNG
                     Y"
     gene            complement(7073..7702)
                     /locus_tag="VEJ00_14510"
     CDS             complement(7073..7702)
                     /locus_tag="VEJ00_14510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXX02425.1"
                     /translation="MEKKITVLLVDDHSLVRRGFRRILEDEPDMEVVGEAGDGEEAVK
                     LAGQLHPRVVVMDCALPGMNGLQATRQILDDSPDTAVLMLSMHSESTWVRQAIEAGAK
                     GYILKNALDLELGAAVRKVAAGETVFDPQVEQRSVLKGERNGGLTQRELEVLQMIVDG
                     KSNKEIALALDLSANTVAVHRANIMNTLGIHKTAELVVYAIRAGLVNVP"
     gene            complement(7668..>8444)
                     /locus_tag="VEJ00_14515"
     CDS             complement(7668..>8444)
                     /locus_tag="VEJ00_14515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXX02426.1"
                     /translation="FLAATLLAILLSSLSMIRWNRRLFAQLAEISERRSELAQKLIAT
                     QESTLRHISRELHDEFGQILTAVGSMLRRAGSHAPEGSNLRAALQEVLQTVQSTLDSV
                     RSLSQALHPVMLEEAGLESTLDWYIPTVERQTGIAISYVKQGQPFPVESAARVHIYRI
                     LQEALNNVARHSGARQAWVRLQFLPDALELEVEDHGIGFAERAKRQGIGLVAIRERSE
                     LMGGRVEFSRPPEGGTLLHLVIPREKAESYGKEDHSLAGG"
BASE COUNT         1601 a         2486 c         2538 g         1819 t
ORIGIN      
        1 agaaacgctg gacaacgcgc tctatgatgc gtttggccaa cggcaggtgt caacgatgta
       61 ccgcccgctg aatcagtatc acgtggtgat ggaagtcgcg ccgcagtttc agcagactcc
      121 ggagtctctg cagaacatct atctgcgctc taccgccgga agtcccgtgc cgctgtcagc
      181 ctttacccac tttgagccgt cgaatactcc gctggccgtg aatcatcagg cacaactgcc
      241 ttcagcgacg atctcgttca acctggcgcc cggcgtctct ctcggccagg cgacagacgc
      301 gatcgagcaa gccgagcgga cgattggctt tccaccgact atccaggcga gttttcaagg
      361 taccgcggca gcgttccagg actcgaaatc caacatgctg atcctgatca ttgccgcgct
      421 cggcgccgtc tacattgtgc tgggcatgct ttacgaaagc tatgtgcatc ccatcacgat
      481 tctctctacg atcccttcgg cgggtgtggg agcgctcctg gcgctgcttt tgaccggcaa
      541 cgaactgaac gtaatcggaa tgatcgggat catcctgctg atcggactgg tcaagaagaa
      601 cgccatcatg atgatcgacg tggcgctgga cgtggagcgg acgcaaggca agaagccggt
      661 cgaggcgatc tatgacgcct gcatcctgcg cttccggccg attatgatga cgacaatggc
      721 cgcgcttctc ggggggttgc cgctggcgct gggcagggga accgggtccg agctgcaccg
      781 cccgctggga atcacgatcg tcggcggctt gattgtcagt caggccctga cgctgtttac
      841 gacgccggtg gtttacgtgt atcttgaccg gctgcggcta gcacttcgcg gcggtgtgga
      901 ccgtgccctg ccagacgctg gcgcgcctag cccggcgcac tgagcgcatc gatttcggac
      961 gacatgttcc ttagggccgt gggttccttc tgcactttcc ctttttgagg gccaggccgt
     1021 acactagaac gcctgtgcaa cctcggtgaa gaaaggttgc atcgcagcta ggacgggcgc
     1081 gattctgccg cctgtcgagg ccgtgcggat caaacgtcta tgccagcacc gcacgctgca
     1141 gctgtccaag atttcagatt cttccgtatg atagaggaca caagaacagg attagggaac
     1201 gcaaaaccat cgcccctgga accggcgggg ccgaccagcg acttcccatt tcgcttcgaa
     1261 aactattgca ggagtttggg atgctaaaga aagcagcggc tctgctcgtc gtttgtgtga
     1321 gcgtagccac atgggtcagt tgtgggacta ccgtaagcca cttcgtctac gcgtcgacac
     1381 cagcctccaa ccagatcgct gcctaccgcg aagatccaaa ctctggcgtt ctgactgcac
     1441 tcgcgggaag cccattcgag gcaggcaacg gggtcgaggc gatcgcgctg catccaacga
     1501 agaagttctt gtatgccgta aactcgttct ccagcgatat ctccttgttt accgtttcga
     1561 ccctgggtgc gattaccgag atgatgccgc gtacgcccgt tacacccgga ggtacgaacc
     1621 cgaaattcct cgccatcgat accgccggaa gcttcctgta tgtcgggaac atcggctcga
     1681 acaacatctc cgtcttctcc atcgacgcca gcagcggaga ccttaaagaa atcagcgggt
     1741 ctcccttccc gattgggatg actccgttga acatggcgct ggcgcctaac ggaaacgtcc
     1801 tctatgtcac cggaggtggt tcaggccagc aaggtatcat ccagatcttt actctcacgg
     1861 caggtgttct ttcgccgatt gccggaccag ctgttcctcc gggaagaagt cccgtcggtc
     1921 tagtcatcaa ttccggtgga tcgatcctct acaccgccaa tcgtctggac aatacgatct
     1981 ccggatactc cattgccacg gacggctccc tgtcactgat cagcggcttt ccgctgagtg
     2041 tgtcgggttt gtcgggcccg gatgcgatgt tgatcgacaa ttccggaaag tatttgtacg
     2101 tggccaacgg actctcgaac aatctgacgg cactttccat cagctccggc ggggcactga
     2161 cgcttctggg caactcgccg ttcggcaccg cttcgcagcc caactttata gccagcgacc
     2221 caagcgggaa gcatctcttt gtgggcaacc aggtcgggcc ggcgatcgag tctttcaggc
     2281 tcgatacggg tacggggacg ctgacgtccg tggcttcata ctcagttggc aatgtaccca
     2341 gctcgatcgt ggtagggcag taggctgagt cgacaatgat cggccagaac cagggcggaa
     2401 agtgatttgg ttttaacaaa cgcttgacaa atcacggcat gtgcgttggg acaatggact
     2461 agccgtacga aagtcgcggc cggagccgcg cttgcccaaa ccgtagcccg cagtcagggg
     2521 atgtcgggta cactagggca ccgtcttcgc gccgggagcg gacccacaac tcatgagaat
     2581 ttcgagcgcc gccagcgcct ttcccaaaca ctactattcc cagaagttcc tgctgcaaaa
     2641 gctgcaagaa tactggggag acagcctgcg caacccgcaa atgctggcgc gtttgcatcg
     2701 caacgtcgcg gtggaagggc gtcatcttgc catgccggcc gagaaatact acgagatgac
     2761 cacctggggc cgggccaacg atatctggat cgaagtcggg caggaactgg gggaacaggc
     2821 gctctgtcgc gctttgcatc atgccgggct ggaacccagc caactggggg cgctgttctt
     2881 cacctccgtt acgggcattt ccagcccatc catcgatgcg ttgctgatca accgcatggg
     2941 gctgccggcg aacatccggc gagtgccgat tttcggactt gggtgcgtcg cgggagcggc
     3001 cggaatcgcg cgtgcagcgg actacgtacg tgcctacccg tcgcaggcag ctgcgctggt
     3061 ttcggtggag ttgtgctcct tgaccattca gcgcgacgat ctttcggtcg ccaatctgat
     3121 ttcctcgggc ctgtttgctg atgggtcggc cgcggtcatt gtcaccggcg acgaactcga
     3181 ttccactgga cctcagattc tggcaacccg ctccatcttc tatcccgata ccgaggagat
     3241 gatgggatgg aacatctcgg aaaagggctt ccgcattact ctctcgccgc aagtgccggt
     3301 tttgattcga gaacatctcg ggcatgacgt ggacactttc cttgccgatc aaggctatca
     3361 acggagcgat atcgggagct gggtgttgca tacgggaggg ccgaaagttc tggaagccac
     3421 cgcggctgca cttgaattga acaatggaca actggatgct tcctgggatt gcctgaggaa
     3481 agtcggcaac ctgtcgtccg cttcggtgct ggtcgtactg gaagacgtga tgagaaaccg
     3541 gcgtccgcag ccgggcacct tgggacttct tgcggctatg ggacccggct tctgttccga
     3601 gctcgtattg ctgcaatggt agattcctga tatgagtcaa cgtgtaccgg ctacgaccga
     3661 tgtttttgtc atcggaggcg ggcccgctgg gttggctgct gcgatcaccg cgcggcagcg
     3721 tgggcttagt gtggtcgtgg cagacggtgc ccagcctcct gtcgacaagg cgtgcggcga
     3781 aggattgctg cccgacgcgc tggcggcact ggagcggtta gggattcacc tgtcggcgac
     3841 atacgctcat ccattcggcg gcattcggtt tgtcggctca accctttcag ctgacgcccc
     3901 tttccctgcg aaaagttacg gtctttctct tcggcgcacc gctctccatc gcgtcatgat
     3961 ggaacgtgcg tcacagttgg gcgcggtcct gctctggcag acggcggtga ccggcatctc
     4021 ttccgacgga gtccacactg gaaaaggatt ggttagcgcc cgctggatcg tcggcgcgga
     4081 cggctgcaac tcccgtgtgc ggcattgggc aggcctcgat cgtggatcgc gatttcgcta
     4141 ccgctatgcc tttcgacggc attaccgcgt tgcaccctgg tcggatttca tggaagtcta
     4201 ttggggcgat cgttgtcagg gctatgctac cgccgttagc ggcgatcagg tttgtgtagc
     4261 attggcatcg cacgaccccg attttcggct ggaggacggg cttcgagcat tgccacgttt
     4321 aggtgcccga ctggccgggg cggagccggc atcagcggaa cgaggcgcgc tgaccggaaa
     4381 tcggacgctg agacaggtgc ggcggaacaa tgtcgtactt attggcgacg cctccggcat
     4441 ggtggacgcg attaccggag aaggccttgg cctggcgttc agacaggccg tcgtgctggc
     4501 ggaatgcctt gcgtccggag acatggcgcg atatcaggcc ttgcactggc ggtttgcgct
     4561 gcgtccgctg tggatggcac ggctgatgct taccctcgat ggccgaccgc ggctgcaaca
     4621 tcgcacgctg caggttttcc agaagcggcc tgaggtcttc cagcacttac tggagctgca
     4681 cgtcggtgct gcatcccctc ttgatgttgc cctcgacggt ctgactctgg gatgggggct
     4741 gttgaccgct tgagcgcaat aaggtcaggg aaatgttggg caggcgtaac gtcatccggg
     4801 attctcctcc tgtcgatggt tgcaatcgcg ccggcgacag cccagcaagc ttctctcacc
     4861 atcgatccgg taaaaaccac gctcagcttc acgttgggcg cagccctgca tacagtccac
     4921 ggatcgtttc aagtcaagcc gggaacgctg gagcttaacc ccgtctcggg acaggtctcg
     4981 ggagagatcg acgtggatgc aaagagcggg gaaaccggaa gcggaatgcg ggatcgcaag
     5041 atgcataaag acgtcctcga aagccagcgt tatccggaga tttcctttcg tcccgaccgg
     5101 gtcgagggtg ctgtcgcgag tctgggaaga tcgacggtga aagtgcatgg aatgtttcgc
     5161 attcacgggg tcgaccgtga aatcacggtt ccagctgtcg tggaaatggc tggcgaccat
     5221 tggtccgcga cggtccattt cacggttccc tatgcgaagt gggggatgca gaatccgagt
     5281 acgctctttt tgcgggtgag tgattccgtg gagattgagc tggcggtggg cggcagcgcc
     5341 gcccaacacg gcgctgcggc tgccagctcg gatcagtagc cgttctgggg cgtgattccc
     5401 ttcccttcgg tcacttcata gcagcggcct gcggcgacgt ctttatattc ttccgtgcga
     5461 ccgctcggcc aatcgatgac cacccgctcg atccgatcgc gcttttccag accaaaggtc
     5521 accggaagct ctgactgcga gaggtagctg gatccacctt tcaccattcg agactgcgtg
     5581 acgccccctc ccgtgatgtg cacagtcgct ccgatcgcgt cccggttgga ttttgttcct
     5641 accagacgaa accggatgct gcggttcccg ttcagcacgt cgttgcgata aagatatgcc
     5701 gggccgttgt tcgtggtgag caggagatcc agatcaccgt cgcggtcgaa atctccatag
     5761 gccaggccgc ggccgacttt cggttgatcg aagcctcctc cgacctctgt cgcaatgtcg
     5821 cggaaggtat cttttccacc gttgagaaaa agctggggag gttgcgcgta gccgacattt
     5881 ccgcgaatgt tgcgcacggt ctcgtcgatg tgtccgttgg ccacggcgaa gtcaagccag
     5941 ccatccaggt tggcgtcaag aaaaacgcag ccaaagccca aactgttccg tgaagccatt
     6001 ccgacgccgg actgcggagc aatatcttcg aagctcttac tgttcgcccg gtacaggccg
     6061 atcatttcgt tgtcgaagtt ggtgatggca actccgggcg tgcccgagtt ttcgaaatcg
     6121 gccacgtcca cacccatgcc ggcgcgtgcc ttgccttcac tgctaaaggc cagtccggct
     6181 tcgacggcga catccttgaa gctgccatta cgctggttgc ggtacagctt gttgggctgc
     6241 gtgtcgttgg ccacgagcag atcgggccag ccatcgcggt tgtcgtcgag caaggctacg
     6301 cccaaggatt ttgaactgct atcgaagatg ccgctggtgg ccgtgacgtc ttcgaatgtg
     6361 ccgtcgccac gattgtgaaa gagccagcag gtagctccgc ggtaagcttc gggagtgcaa
     6421 tacgatttgt gttttccatc gaggctgcag aagacatcct gctccggcga ccacttcacg
     6481 tagttgcaga caaaaagatc aaggagtccg tctcggtcat agtcaaacca gagggctgat
     6541 gtgctgaagc cttcgcgctt gccgagtccg ctggagttag tcacatcgat gaaggtcccc
     6601 ttgccggtgt tgcgaaacag gcggctctgt cccacgcaag tgaccagaat gtccgggaag
     6661 ccgtcgttgt tgtagtcgcc gacggcgact cccatgccgt acagctcgat atcgagcccc
     6721 gcgtgcgcgg ttacgtccgt gaacgttccg ttcccattgt tgcggtacag gcgcagcgtt
     6781 gtgcgcttct tcttgtgtcc cggccagtcg gcgccgttga taagcaggat gtcctgccag
     6841 ccgtcacggt catagtccag aaaggcgcag ccggagccga gtgtttccgg taggaatttg
     6901 ccgccgaagg ctccgctgtt gtgctggaac tcaactccgg cctggctggt aacgtctgcg
     6961 aatcgaaaac cgggggatgc acctgctccc gtctgggccg cgggacgggc gaggcgaggc
     7021 caatacggcg aagccgccac tgcccccatt ccccatagga aagacctgcg attcacggaa
     7081 cgttcaccag accggcgcgg atcgcgtaca ccaccaactc cgcagtctta tggatcccca
     7141 aagtgttcat gatgttggcg cggtgaacag caacggtgtt ggcgctgaga tcgagggcga
     7201 gggcgatttc cttgttcgac ttgccgtcga cgatcatctg caggacttcg agttcgcgct
     7261 gcgtcagtcc gccgttgcgc tcccctttga gaacgcttcg ttgttcaacc tgcgggtcga
     7321 atacggtctc gccggcggcg actttgcgaa ccgctgcccc gagttccagg tcgagagcgt
     7381 ttttcaaaat gtaccctttc gctccagctt cgattgcctg acgcacccag gtgctctcgc
     7441 tatgcatgct gagcatcaga actgccgtat caggcgagtc gtcgaggatc tggcgcgttg
     7501 cctgcaatcc gttcatccct ggcaaggcgc agtccatgac taccactcgg ggatgcagtt
     7561 ggccagcgag cttgacggct tcttccccgt ctcccgcttc gccgaccact tccatatcgg
     7621 gctcgtcttc caggatgcgc cggaagccgc gccgcaccag gctgtggtca tccaccagca
     7681 agactgtgat cttcttttcc ataagactct gctttctccc ggggaatgac aagatgcagc
     7741 agcgtgccgc cttcaggggg acgagagaac tcgactcgcc ctcccatcaa ctcggaacgt
     7801 tcccggatgg ccacgaggcc gataccttgt ctcttcgccc gctccgcgaa gccgatgcca
     7861 tgatcttcca cttccaactc gagagcatcg ggcaggaact gcaagcgaac ccaagcctgc
     7921 ctggctccag agtgacgcgc gacattgttc agggcctcct gcaggatgcg gtaaatgtgc
     7981 accctggcag cactttcaac aggaaagggc tggccttgct tcacatagga tatggcaatc
     8041 cccgtctgcc tttctacggt ggggatgtac cagtcgaggg tgctctcgag tcccgcctct
     8101 tcgagcatga ccgggtgcaa ggcctgagag aggctgcgga cgctgtccag cgtgctctgg
     8161 actgtctgaa gcacttcctg caaagcagcc ctcaggtttg agccctctgg ggcgtggctt
     8221 ccggcccggc gcagcatcga accgaccgca gtgagaatct ggccaaactc gtcgtgcagt
     8281 tcgcgcgaga tgtggcgcaa ggtggattcc tgggttgcga tcagtttttg cgccagctcg
     8341 ctgcggcgct cggagatttc cgccagctgc gcgaacaatc gccggttcca gcgaatcatc
     8401 gagaggctgc tcagcaggat cgcaagcagg gttgctgcaa gaaa
//
LOCUS       DASPUL010000627         5049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1347272, whole genome shotgun sequence.
ACCESSION   DASPUL010000627 DASPUL010000000
VERSION     DASPUL010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347272"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VEJ00_14520"
     CDS             complement(<1..610)
                     /locus_tag="VEJ00_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXX02427.1"
                     /translation="MSNGASKDDLRIRLLIVDDEQSIRKLCMTVGEALGFVCLEAESG
                     DSAMALLEEQPAHMVLTDLVMPHMSGLEFLEKVKKLLPRTEVALMTGHGSIETAVQAM
                     KLGAYDYITKPFSPLEELRLFLRRMAEKVRLVEENEFLRQRMDSETAVHGIVGSSVRI
                     QEVLRMVARLKDTRTPVLIYGESGTGKELVARAIHFRGAFAAR"
     gene            complement(607..1008)
                     /locus_tag="VEJ00_14525"
     CDS             complement(607..1008)
                     /locus_tag="VEJ00_14525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXX02428.1"
                     /translation="MSLHAVQPSPLPVLLIEDEPAVMAYVRAALERSGYPVVCCESGA
                     DALRLLEDGSFLGVVSDMRTPGGVDGGQVHAWIAQHRPDLVSRVVFITGDIANEETVA
                     TLRQTGAPCVEKPFRVQQFIDVVSRTLGKAL"
     gene            complement(1005..4946)
                     /locus_tag="VEJ00_14530"
     CDS             complement(1005..4946)
                     /locus_tag="VEJ00_14530"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXX02429.1"
                     /translation="MNREPLLSRVGGGPGVRDASPGGSVSPGSDEAFQRLLLRIAARA
                     SERADAAALIQLFCQATREFFQVPGVYFWRCLSGDELVGEYAEGKLAERFAGIRIRPE
                     DSAVTVEAVRQRRTIFANRVLSSAPVLAQFGGVSLLAAPLVVFNEVIGAVTFLHDRDS
                     TFFNEDLAAKATILAGQLGSLLEATRLSEASREEHRRAEILADVAQALHATPDVTSVL
                     EALADRLRRLLRTPLVCVFLKREGPFELRAVAGDTPQLANSARARFDRQTLRFVADLA
                     QRALAAGELVTVSIGADAHALGSQVSSGMLIAAPLRTSRTQGAILVYPRPDGVFTADE
                     KALVSAVAGFGAVAVAHAELHVTAHAQAKELHQLLEISSQLSASADLEQFLQAFVVHA
                     ADFLGYGRCFIALSEGEHFQVRYGVERGEPRRVDVRFPEGMATHALRAKEVYWTDDAT
                     QSPGNNLELVTKYHVHQFLAVPLLGSGGQVLGMFAVLDRLDGTGISQEDIRRARALSN
                     QAAVALEVARNLHLSEQNRRRAEALIELAREIDGALHLPEFARRFVARAAELGGCQEG
                     WLAVRQEGHFQVVAVYPPPDTAGAGPSGGAASAETARPVLDSEAEHALAAALPEFVTR
                     HSEIVVSGPAEQLLGPEAASRLRWTDCTLVRLRGASGELAGLLGLSGRSHALENEDRV
                     FLQTMAGHAAMALENARLFTRIEQANRHWMAIFDAITDFIVVHDQADKVLRVNRSLAA
                     MIGVPPAELIGVDMRSLLALTSDVAPYSCPFCRAMADDADEFVHPVFDRTYLVSTSRV
                     HSASSSPTGEAVQTIHVLKDITDRREAERRYRELFDNIQEGLFFSTPDGRFIEVNDAM
                     VRMLGYSDREELLQVDIPSQLYFSLEQREHHAAVLKETGHLRNFEATLRRKDDSPIHV
                     LINAFGLYDNQGRLLQIRGLMLDITGLRTSQSELQRERDFSGKILSNTQSLILVADTA
                     GLISYANRRWAAAGFEQRDLLGRPLLELAAPGYVRPLADALQQTLNAQQVDNLELQIM
                     RGGGVAGQFSANLSPMRDEQGHVTSIVVVLTDITDSAVLRDKLVHAEKMAAVGQLVSG
                     VAHEVNNPLTAILGFADLLMENPEMPEAARKDLRVILQEAQRTKQIVQNLLSFARQMP
                     PERNAVQLNSILRRTIQLRSYDFTSHGVEVIEHLDEGLPEVIGDAHQLQQVFLNILNN
                     AYDAVHEVGRPARIEIMTTKADATVEVSFRDNGNGISHPDKIFDPFFTTKEVGKGTGL
                     GLSICYGIVKEHGGEILCHNNLGGHGATFVVRLPAAEHTASLGVAAGVIQK"
BASE COUNT          940 a         1524 c         1721 g          864 t
ORIGIN      
        1 ggcgcgctgc gaaggcgccg cggaagtgga tggcgcgagc gacgagctcc ttgccggttc
       61 cgctttctcc gtagatgagc accggagtac gcgtgtcttt caagcgggca accatgcgca
      121 ggacttcctg aatgcggacc gaggagccga cgatgccgtg caccgccgtt tcggaatcca
      181 tgcgctggcg cagaaattcg ttttcctcga ccaggcggac tttttccgcc atgcgacgca
      241 gaaacaggcg aagttcctct agcggcgaga aaggtttggt gatgtagtcg taggcgccca
      301 gcttcatggc ctgcacggcg gtctcgatgg agccgtggcc ggtcatgagg gctacttcgg
      361 tgcggggcaa aagcttcttc actttctcga gaaactccag acccgacatg tgaggcatga
      421 cgaggtcggt cagcaccatg tgtgccggct gctcttccaa aagagccatt gccgagtcgc
      481 cactttcggc ctcgaggcac acgaagccca gcgcctcgcc caccgtcatg cagagtttgc
      541 ggatgctctg ctcgtcatcg acgatcagca ggcgaatgcg caggtcgtct ttgctggctc
      601 cgttgctcac agcgccttcc ccaaggtccg cgatacgacg tcgatgaatt gctgcacgcg
      661 aaacggcttc tccacgcagg gagcgccggt ctggcgcagg gtggcgacgg tttcctcgtt
      721 ggcgatgtcg ccggtgatga aaacaacgcg gctgaccaga tcggggcgat gctgtgcgat
      781 ccaggcatgc acctggcccc cgtcgacgcc gccgggcgtg cgcatgtcgg agaccacgcc
      841 cagaaaggat ccgtcctcga gcaggcgcag cgcgtccgca cccgattcgc agcacacgac
      901 cggataaccg ctgcgctcga gcgcggcccg aacgtaggcc atgacagcgg gctcatcctc
      961 gatgagcagc acgggcaggg gagagggttg tacagcgtgt aggctcattt ttgtatcact
     1021 cctgcggcca cgcccaggga agcggtgtgc tccgccgcgg gcaggcgcac cacgaaggtg
     1081 gcgccatgcc cgccgagatt gttgtgacag agaatttcgc cgccatgctc cttcacgatg
     1141 ccgtagcaga tgctcaggcc gaggccggtc cctttgccga cttccttggt ggtgaagaag
     1201 gggtcgaaga tcttgtcggg atgggaaatc ccgttgccgt tgtcgcggaa agaaacttcg
     1261 accgtagcat ccgccttggt ggtcatgatc tcgatgcgcg ccgggcgccc gacttcgtgc
     1321 acggcatcgt aggcgttgtt cagaatattg agaaagacct gctgcaactg gtgggcgtcg
     1381 ccgatgacct caggcagccc ttcatcgaga tgctcgatga cttccacgcc gtggctggtg
     1441 aaatcgtagg agcgcagctg aatggtgcgg cgcaggatgg aattgagctg cacggcgttg
     1501 cgctcgggcg gcatctggcg ggcgaagctg agcaggttct gcacgatctg cttggtgcgc
     1561 tgggcctcct gcaggatcac ccgcagatcc ttgcgggcgg cttcgggcat ctcgggattt
     1621 tccatgagca gatcggcgaa cccgagaatc gccgtcagcg gattgttgac ctcgtgggcg
     1681 acaccggaga ccagttgccc gaccgccgcc attttctcgg catgaacgag cttgtcgcgc
     1741 aagacggcgg aatcggtgat gtcggtgagc acgaccacga tgctggtgac gtggccctgc
     1801 tcgtcgcgca tggggctgag attggcggaa aactgcccgg ccacgccgcc gccccgcatg
     1861 atctgcagtt cgagattgtc gacctgctgc gcgttcaagg tttgctgcag cgcgtcggcc
     1921 aggggccgga cgtagccggg cgcggccagt tccagcagcg gacgccccag cagatcgcgc
     1981 tgctcgaagc cggcagccgc ccagcggcgg ttggcgtagc tgatcaggcc cgcggtatcg
     2041 gccaccagaa tcaggctctg cgtgttgctg agaatcttgc ccgagaagtc gcgctcgcgc
     2101 tgcagctccg actgagaagt gcgcaggccg gtgatgtcga gcatgaggcc gcgaatctgc
     2161 agcaggcggc cctggttgtc atacaggccg aaggcattga tcagcacatg gatgggtgaa
     2221 tcgtctttgc ggcgcaacgt ggcttcgaag ttgcgcaggt ggccggtttc cttcaacacc
     2281 gcggcatgat gctcgcgctg ctccagcgag aaatagagct gcgaggggat gtcgacctgc
     2341 agcagttctt cgcggtcgga gtatcccagc atgcgtacca tggcgtcgtt gacttcgatg
     2401 aagcgcccgt ctggcgtgct gaagaacagg ccctcctgaa tgttgtcgaa cagctcgcgg
     2461 tagcggcgtt cggcttcgcg gcggtcggtg atgtctttca ggacatggat cgtctggacg
     2521 gcttcgccag tcggggaaga agaagcgctg tgtactcgcg aagtggagac caggtaggtg
     2581 cgatcgaaaa ccgggtgcac gaattcgtcg gcgtcgtcag ccatagcccg gcagaagggg
     2641 cacgaatagg gcgcgacgtc gctggtgagg gcgagcagcg agcgcatgtc gacgccgatc
     2701 agctccgccg gtggcacgcc gatcatcgcc gccagcgagc gattcacccg aagcaccttg
     2761 tccgcctgat cgtggaccac gatgaaatcg gtaatcgcat cgaagatagc catccagtgg
     2821 cggttcgcct gttcgatacg ggtgaacagg cgggcgttct cgagagccat ggcagcgtgg
     2881 ccggccatgg tctggagaaa gacgcggtct tcgttctcga gtgcgtgcga acgtccggac
     2941 aggccgagca acccggccag ttcgccgctg gcgccgcgca ggcgcaccag ggtgcagtcg
     3001 gtccagcgca ggcgggaggc agcctccggg ccgagcaact gctcggcggg accggaaacg
     3061 acgatctcgg aatggcgggt cacaaattcg gggagggccg ccgcaagggc gtgctccgcc
     3121 tcggaatcga gcacgggacg ggccgtttcc gccgacgctg cgccaccgga aggacctgcg
     3181 ccggctgtgt cgggcggagg atagaccgcg actacctgaa aatggccttc ctgcctcacc
     3241 gccagccagc cctcctggca gccgccgagt tcagccgcgc gggcgacgaa gcgacgggcg
     3301 aactcgggca ggtgcagggc gccatcgatt tcgcgggcca gctcgatcaa ggcctcagcg
     3361 cggcgccggt tctgctccga caaatggaga ttgcgggcga cttcgagcgc gacggctgcc
     3421 tggttcgaca gcgcgcgggc gcggcgaata tcctcctgcg aaatgccggt gccgtcgagc
     3481 cggtccagta ccgcgaacat gcccagtacc tgcccgcccg atcccagcag cggcacggcg
     3541 agaaattgat gcacgtggta cttggtgacc agctccaggt tgttgccggg actctgggtg
     3601 gcatcgtcgg tccagtagac ctctttggcg cggagggcgt gcgtggccat gccctcggga
     3661 aatctcacgt caacacggcg cggctcgccg cgttccacgc catagcgaac ctggaaatgt
     3721 tcgccttccg agagggcaat aaagcagcgg ccatagccga gaaagtcggc agcgtggacg
     3781 acgaaggcct gcagaaactg ctcgagatcg gcgctggcgc tgagctggga agaaatctcg
     3841 agcaactggt gcagctcttt ggcctgagca tgcgcagtga cgtggagctc ggcgtgggca
     3901 acggcgacgg cgccgaagcc ggcaacagcc gaaaccaggg ccttctcatc ggcggtgaaa
     3961 acgccgtccg gacgcgggta gacgaggatc gcgccctggg tgcgcgaggt gcgcagcgga
     4021 gcggcaatca gcattccgga agaaacctga ctccccagcg cgtgcgcgtc agctccgatg
     4081 gaaacggtga ccagttcgcc tgccgcgagc gcgcgctgcg ccaggtcggc gacgaagcgc
     4141 agagtctggc ggtcgaagcg ggcgcgcgcc gagttggcca gctgcggcgt gtcgccggcc
     4201 acggcgcgca gttcaaacgg accttcccgc ttcagaaaaa cgcagaccag cggagtccgc
     4261 agcagccggc gcaggcggtc ggcgagagct tcgagcacgg atgtcacatc gggcgtcgca
     4321 tgtagggcct gggcgacgtc ggcgagaatt tcggcgcggc gatgttcctc gcgcgaggct
     4381 tcactcaggc gcgtggcttc cagcaggctt cccagttgtc ccgccagaat ggtggctttg
     4441 gcggccaggt cctcgttgaa gaaggtgctg tcgcgatcgt ggagaaacgt caccgcaccg
     4501 atgacttcgt tgaaaacgac gaggggggcg gcaagcagcg atactccgcc gaattgcgcc
     4561 agcacgggcg cggaagacag cacgcggttg gcgaagatgg tccgccgctg ccgcaccgcc
     4621 tcgacggtca cggcgctgtc ttcggggcga atgcggattc ccgcaaaacg ctcggccagc
     4681 ttgccttcgg catattcgcc caccagttcg tcgcctgaaa ggcagcgcca aaaatagacg
     4741 ccggggacct ggaagaactc gcgcgtagcc tgacaaaaca actgaatcag ggcagcggcg
     4801 tcagctcgct cgctggcgcg ggcggcgatt cgcagaagga gacgctgaaa ggcttcatcg
     4861 gaaccgggag acacactccc gcccgggctg gcgtcccgca cgccgggccc gccaccgacc
     4921 ctcgacagca atggttcgcg attcacaagt gattgaatct aatgggaaat tacaggtcat
     4981 ctcaatttga gtcaagggaa atgggggaag gggtgccttg gtgtgaaatc ccgagaattc
     5041 ccgtgagac
//
LOCUS       DASPUL010000628         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_393713, whole genome shotgun sequence.
ACCESSION   DASPUL010000628 DASPUL010000000
VERSION     DASPUL010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393713"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VEJ00_14535"
     CDS             <1..865
                     /locus_tag="VEJ00_14535"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXX02430.1"
                     /translation="EGAGVHLNRAFGFGNTKDFDPFLLLDDFRNDVPEDYLAGFPWHP
                     HRGIETITYVLAGTVEHGDSMGNNGVIGAGDVQWMTAGSGIIHQEMPKGDRTGRMHGF
                     QLWGNLPSGLKMTAPRYQEVKAEEIPQIVDDDGTHVRVVCGEFWGKKGPVDGVAADPI
                     YLDVSISPGKRKTLPVETTRHAFAYVFAGNGRFCNASGPLAVPTEAVKWLDTTPPVEA
                     DNRSLILFDRGDEVTVQAGSDGIRFLLVSGKPLEEPVAWYGPIVMNTQEQLREAFTEL
                     QEGTFLRVGKK"
     gene            1009..2505
                     /gene="xylB"
                     /locus_tag="VEJ00_14540"
     CDS             1009..2505
                     /gene="xylB"
                     /locus_tag="VEJ00_14540"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HXX02431.1"
                     /translation="MDVGTGGTRALILGEEGRVVASATEEHAPFASPEIGWAEQHPED
                     WWRACCVAIPKALKSSGLGAGDIACVGFSGQMHGAVMLDEQGKVVRPALIWCDVRTEP
                     QCRELTEKIGAAQVIQATCNPALVNFTLTKLMWVREHEPENWKRVRSIMLPKDYVRFR
                     MTGERATDVADASGTLLLDVAHRRWSQVMVDAAGVDERFLPALFESPDVCGSVNADGA
                     RATGLREGTPVVAGAGDQAAGAVGMGIVAPGTVSATIGTSGVVFAAAERPALEPQGRL
                     HTFCHAIPGRWHAMGVTQAAGLSLRWFRDQFGVGMSDTKGKAGARDPYELLTDEAEKV
                     PPGCEGLLWAPYLMGERTPHLDPSARGALVGLTASHTRAHVIRSILEGVALSLRDTFT
                     LFDEMKVAVKKIRLGGGGARSPLWRQIQADVYGREVEIVEADEGAAYGAAILAGVGAR
                     VWNSVDEACSAVVRVAATIPPQPDAVNMMNASYARYRRLYPAIKNVLG"
     gene            complement(2538..>2959)
                     /locus_tag="VEJ00_14545"
     CDS             complement(2538..>2959)
                     /locus_tag="VEJ00_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXX02432.1"
                     /translation="ARKKYSAVAQRRSPMAHQHPPRFLKIVDDARTRVRETNVDEVKK
                     RLDRGDKFLLVDVREESEFAKDHLPGAIHLGKGVIERDIEVRVPDLNSEMILYCGGGF
                     RSALAADNLQKMGYTNVISMDGGIRGWREKNFPLVKG"
BASE COUNT          550 a          799 c         1040 g          570 t
ORIGIN      
        1 ggaaggcgcg ggagtgcatc tgaaccgggc tttcggtttc ggcaacacca aggatttcga
       61 cccgtttctg ctgctcgatg acttccgcaa cgacgtaccc gaggattacc tggccggttt
      121 tccgtggcat ccgcaccgcg gcatcgagac catcacgtac gtgcttgcgg gtacggtgga
      181 gcacggtgac agcatgggga ataacggggt catcggcgcg ggcgacgtgc agtggatgac
      241 ggctggcagc ggcatcattc atcaggaaat gcccaagggc gaccgtacgg ggcggatgca
      301 tggattccag ctttggggaa atctaccgtc ggggctcaag atgacggcgc cgcggtatca
      361 ggaggtgaaa gcggaggaaa tcccgcagat cgtcgacgac gacggcactc atgtgcgagt
      421 cgtctgcgga gaattttggg gcaagaaggg gccggtggat ggcgtcgcgg cagatccgat
      481 ctatctcgat gtatcgattt cgccggggaa gcggaagacg ctgccggtgg agacgacgcg
      541 gcatgcgttc gcttacgtat ttgccgggaa tgggagattc tgcaacgctt cgggccctct
      601 ggcggtgccg accgaagcgg tcaaatggct ggacactacg cctccggtcg aggcggacaa
      661 ccggtcgctc attctttttg accggggcga cgaggtaacg gtgcaggcgg gaagtgacgg
      721 aattcgcttt cttctggtct cgggaaagcc gctcgaggaa ccggtggcct ggtacgggcc
      781 gatcgtcatg aatacgcagg aacagctgcg ggaggcgttt accgagctcc aggaaggaac
      841 attcctcagg gtgggcaaga aatagcgaat cggcgccgca cgaggctatc cgggcagaac
      901 aacacgccga cagatgggtt tttccattcc agatttccag ccaaagctgt atcgcgcgct
      961 tcgacctgag gcttagcgcg gggggagcag aacatcttta ctctgggaat tgacgtgggt
     1021 acgggcggaa cgcgcgccct gatccttggt gaagaggggc gcgtcgttgc ttccgccacg
     1081 gaagagcacg cgccctttgc ctcgccggag atcgggtggg cggaacagca tcccgaggac
     1141 tggtggcggg cctgctgcgt tgcgattccg aaagcgctca agagcagcgg gcttggagcg
     1201 ggtgatatcg cgtgcgtcgg cttctccggc cagatgcacg gagccgtaat gctcgacgag
     1261 caggggaagg tggtgcgccc ggcgctgatc tggtgcgatg tgcggactga gccccagtgc
     1321 cgcgagttga cggagaagat cggtgcggcg caggtgattc aggcgacgtg taatcctgcg
     1381 ctggtcaatt tcacgctgac gaagctgatg tgggtgcgcg agcacgaacc ggaaaattgg
     1441 aaacgcgtgc gctccatcat gctgccaaaa gactacgttc gattccggat gacgggagag
     1501 cgcgccaccg acgtggcgga tgcctcagga acgctgctgc tggatgtggc acaccggcgc
     1561 tggtcgcagg tgatggtgga tgcggccggg gttgatgagc gcttcttacc tgctctgttt
     1621 gagtcgccgg acgtgtgcgg ctccgtcaat gcggacgggg ctcgagcgac cggcctgcgc
     1681 gagggaactc cggtggttgc gggcgcgggc gatcaggctg cgggcgccgt cgggatgggc
     1741 attgttgctc cggggaccgt cagcgcgacg attggaacct caggggtggt ttttgcggca
     1801 gcggagcgtc ccgcactcga accccagggg cgcctccata ctttctgcca cgcgattccc
     1861 ggacgctggc acgcgatggg cgtgacccag gcggctggac tgtcgcttcg ctggtttcgt
     1921 gatcagttcg gggtgggtat gagcgacacc aaagggaagg caggcgcccg cgacccttac
     1981 gagctcttga ctgatgaggc ggaaaaagtg ccgccggggt gcgagggcct gctatgggct
     2041 ccatatctga tgggcgagcg gacaccgcat cttgatccca gtgcacgggg agcgctggtt
     2101 ggcctcacgg cttcacacac acgcgcacac gtgattcgtt cgatcctcga gggcgtggct
     2161 cttagcctgc gcgatacctt tactttgttc gacgagatga aagttgcagt gaaaaagatt
     2221 cgactcgggg gaggcggagc gcgatcgccg ctgtggcggc agattcaggc ggatgtttac
     2281 ggccgcgagg tggagatcgt ggaagccgat gagggtgccg cgtatggagc ggcgatcctg
     2341 gctggagtag gggcgcgagt gtggaactcc gtggacgagg catgctctgc ggtggtgcgt
     2401 gtagcggcga cgattccacc ccagccggac gcggtcaaca tgatgaatgc aagctatgcg
     2461 aggtatcggc ggctgtatcc ggcgatcaag aatgttttag gctagctcga caatcgggag
     2521 attgcgggac catctcttca cccttttacc aagggaaagt tcttctccct ccagccccga
     2581 atgccgccat ccatggaaat cacgttggtg tagcccatct tttgcaggtt gtcggcggcg
     2641 agagcggagc ggaaaccgcc gccgcagtag aggatcatct cggagttgag atcgggcacg
     2701 cgcacttcaa tgtcgcgctc aatcacgcct ttgcccagat gaatcgcgcc gggaaggtga
     2761 tccttggcga actcgctctc ctcgcgcacg tcgaccagca agaacttgtc gccgcggtcc
     2821 aggcgcttct tgacttcgtc gacattggtc tcgcgcacgc gagtcctggc gtcgtcaacg
     2881 atcttgagga agcggggagg atgctggtgg gccatggggg atctcctttg cgcgactgcc
     2941 gagtacttct tccgcgcac
//
LOCUS       DASPUL010000629         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1347850, whole genome shotgun sequence.
ACCESSION   DASPUL010000629 DASPUL010000000
VERSION     DASPUL010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347850"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..>2159
                     /locus_tag="VEJ00_14550"
     CDS             32..>2159
                     /locus_tag="VEJ00_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXX02433.1"
                     /translation="MQLVEGLPLDRLINQDGLPIEQIVEIAGGLADALAAAHEKGIVH
                     RDLKPANVMVTNEGRVKVLDFGLAKDMRVSSPGDATMTCDSRTQVGVVMGTPAYMSPE
                     QTSGRPLDHRTDIFSMGVLLFEMSTGKRPFGGNSSAELVSAILRDTPASVTDARPDLP
                     SDLARIIRRCLEKDPRHRLQTARDVSNEFRDLARSAMRPLSVSASPRHAPVADSGATR
                     AEEGFWVAVLPFKYAGNNTDLKALAEGLSEEIITGLSRFSYLRVIARGSTGNYSSDSG
                     DIRTIGKELGARYVMEGTLRQAGSKVRVAVQLVEATTGSHLWAETYERAFATESAFAL
                     QDELVPRIVATVADRHGVLPHSMSEAVQAKKSDQLTPYEAVLLSFSYAERITSEEHIT
                     AKACLERAVAKAPGYADAWAMLAMLFCDEYGLGFTLELSLLERALETARRAVKAGPSN
                     HLAFQNLAYAHFLRHEHAPCRNAAERALALNSLDGSNVWFMGLVLAYMGDWDLGCALV
                     ERAMQLNPNFPAKYHFPLVANAYRKGDYKGALNEALKMNLPEVIYTPLLAAASAGQLG
                     EKETAVKALRELLVLQPDMAVIAHGLLGKWFQPELVEHFMEGLAKAGLAVGHVGAMPA
                     TQPVATASGESRAEEGFWVAVLPFKYTGANQDLKALAEGLSEEVITGLSRFSYLRVIA
                     RGSTARYSGESGDVREIGKELGARYVM"
BASE COUNT          451 a          619 c          656 g          433 t
ORIGIN      
        1 ggaagagttc gaaggcgttc acttcctgac aatgcaactt gtcgaagggc tgccgctgga
       61 tcgcttaatc aaccaggacg gccttccgat cgaacagatc gtagaaattg cgggaggttt
      121 ggccgacgcg ctcgcggccg ctcacgaaaa aggcatcgtg caccgtgact tgaaacccgc
      181 caatgtcatg gtcacgaacg aaggccgcgt gaaagtgctg gatttcggac tggcgaagga
      241 tatgcgcgtc tcctctccgg gcgacgccac catgacttgc gatagccgca cgcaggtagg
      301 cgtggtgatg ggaacgccgg cctacatgtc gccggaacag acttccggcc gtccgctcga
      361 tcaccgtacc gacatttttt cgatgggtgt acttcttttt gaaatgtcga ccggaaagcg
      421 ccccttcgga ggtaattctt cagccgaatt ggtttcggcg attctgcggg atacgccggc
      481 gtcagtgacc gatgcgcggc ccgatctccc cagcgatctg gcgcgtatca tccgacgctg
      541 cctggaaaaa gatccgcgac accgcttgca gaccgcacgc gatgtcagca acgaattccg
      601 cgacctggcg cggagcgcaa tgcggccgtt atcggtttcc gcatcgcccc gccacgctcc
      661 tgttgcggat tccggggcga cccgcgccga ggaaggcttc tgggtcgccg tgctaccgtt
      721 caagtacgcg ggaaacaaca ccgatctgaa agctctcgcc gaagggctgt ccgaggaaat
      781 catcactgga ctctcacgct tctcttacct gcgcgtgatt gcgcgcggct cgacggggaa
      841 ttattccagc gattcgggag acatccgtac catcggcaaa gaactcggcg cacgctacgt
      901 tatggaaggg actctgcgcc aggccggcag caaggtgcgg gtcgccgttc agctggtcga
      961 ggcaaccacc ggctcgcatc tctgggccga gacctatgaa cgcgccttcg cgactgaatc
     1021 agctttcgcg ctgcaagacg aactcgtacc tcggatcgtc gccaccgtcg ctgacaggca
     1081 tggtgtgctg ccgcacagca tgagcgaggc agtccaagca aaaaagtccg accagttgac
     1141 tccctatgaa gcagtactgc ttagcttcag ttacgcggag cgcatcacgt cggaagaaca
     1201 catcacagcg aaggcttgcc tggaacgggc ggttgcgaaa gcgccgggtt acgccgatgc
     1261 ttgggcgatg ctggcaatgc tcttttgtga cgaatatggc ctcggattca cactggagtt
     1321 atctcttcta gagcgcgctc tcgaaaccgc tcggcgggct gtaaaggccg gcccctccaa
     1381 ccacctggct tttcaaaact tggcctatgc tcacttcctt cgacatgagc atgcaccatg
     1441 cagaaacgcg gccgagcggg ccctggcgct caattcactg gacggcagca atgtttggtt
     1501 catgggttta gtgttggcct atatgggcga ttgggacctc ggttgtgcac tcgttgagcg
     1561 ggccatgcaa ctaaacccaa actttcccgc aaagtaccac ttccccctgg tggccaacgc
     1621 ataccgcaaa ggtgactata agggtgcgtt gaacgaagcg ctaaagatga atttgcccga
     1681 ggttatttac acgccattgt tggccgccgc gtcggcaggg cagctcggag aaaaagaaac
     1741 agctgtgaag gcgttacggg aattgctggt gctgcagccc gatatggcag tgattgcgca
     1801 tgggctcctc ggaaagtggt ttcagccgga gcttgtggaa cattttatgg aaggtctcgc
     1861 caaggcgggg ctggctgttg gccacgtagg agccatgccg gccacccagc ccgtagcaac
     1921 ggcatccggc gaatcccggg ccgaggaagg cttctgggtc gccgtgctgc cgttcaagta
     1981 cacaggtgcg aaccaggacc tgaaagccct ggcggaagga ttatctgaag aggtcatcac
     2041 gggattgtca cgcttctcat atctgcgggt catcgcgcgg ggttcgacgg ccaggtattc
     2101 cggcgagtcg ggagacgttc gcgagattgg caaagaactc ggcgcacgat acgtgatgg
//
LOCUS       DASPUL010000630         5292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1218801, whole genome shotgun sequence.
ACCESSION   DASPUL010000630 DASPUL010000000
VERSION     DASPUL010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218801"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VEJ00_14555"
     CDS             <1..1246
                     /locus_tag="VEJ00_14555"
                     /inference="COORDINATES: protein motif:HMM:NF022379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXX02434.1"
                     /translation="RGSTRLAEAKENAVRLMTVHGAKGLEFPHVFILRANSNSFPCSY
                     RETLVAFPRELRDEDSITADDDRELQKQEERRLFYVAMTRARDSLRIYARQGRGANKN
                     PDGYMRELIENRGLTRWFKSIHASGAQANLDIFAEALPAYPDQSRTTLWFELPVRDGL
                     YTRLSASAIDTYQRCGLQFKLDRDWHIPAKPAAAMQYGASMHRVLKTYFDSVRLGRPK
                     SDEETIELFRQDLAEAGIAEKYQHELYEKQGINQLREFLTSLRSLPALQVLHTEQPFE
                     IRMGPVTVIGRIDRIDRRADGSVAIVDYKTGKARPQEDADDSLQLSLYAIAAQEKWGY
                     KVGELIFFNLDGNVPVITTRCEADLLSARERVEAAAKGIAAGVFHAKPGNHCNFCAYR
                     SLCPEKEKRIPRTVAIGTAQTN"
     gene            1733..2926
                     /locus_tag="VEJ00_14560"
     CDS             1733..2926
                     /locus_tag="VEJ00_14560"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXX02435.1"
                     /translation="MASFDDVVIISGCRTPVGKFQGSLSEFSAPQLGAMVVREAVKRA
                     GVDPAQVDECIMGNVVSAGLGQNPARQAAIFGGLAPTTGAMTINKVCGSGLKSVALAA
                     QAIQTGNSSIVVAGGMESMTNAPYLLPQARKGYRLGNAQVVDSMVHDGLWDIYNDYHM
                     GITGENVAEKYGISREEQDEFALNSHRKALAAIKECRFKSQIVPVEIPAKKKGAPPTI
                     FDKDESPREDTTIEVLRALKPAFKKDGTVTAGNAPGVNDGAAAVVVTSAQRAKELGAK
                     PMVRIVAQATSGVEPKWVMMAPVGAVRKIWEKTGWKNEDVDLYELNEAFSVQALGVMR
                     ELGLNPDKVNVNGGAVAIGHPIGASGARVLVTLIYEMIRRDVRRGIAALCLGGGNAVA
                     MAVER"
     gene            complement(2931..5045)
                     /locus_tag="VEJ00_14565"
     CDS             complement(2931..5045)
                     /locus_tag="VEJ00_14565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXX02436.1"
                     /translation="MTPFAAQTPPPARPPGKLEQEESQLWRWLLFFMVLLAVGFAALA
                     WERLQDLPYHLGPVALGVLVLSVLLAVYAYGRRREVTELKGLLQNLQEHVGAAPSEEQ
                     LDQLSQVIMRSQRSFKELIDSFDDPACAVSLDGTLRTVNRRVAEMAGLHYSELVGHKI
                     YDFIDEPTREEMERGLGRFLEKKRWAGMVQIRLKNSSRPLYFDCVMNAIVKGDEVVGA
                     SMLGRDVTEQREKELRFTELFETLQEGAYFSSPEGKLLDANPALVSMLGYKEKRDLLA
                     VEPAALNFDPGQDPVLGRAVDDRGGVRTREIKLRRKDGSAAVFLDSSRAVWDAAGNII
                     RYQGTLVDVTEKRAMERQLAQQEEFRRQLLESFPDLILVVDLEGRYTFVSSRVGDLLG
                     CKPEDMLGKKITDLEASSSELSLLYQTIVSGKEAFGSAEYAARHHDGSWRTMRAAGSQ
                     LFAADARISGVIFSVRDITIERKLEQQVVQSERLAAMGAMIGGVAHELNNPLTSILGV
                     SELLQDSETNETTRKQLAMLQQQARRAAEIVQNLTYFSRPPAPGKSRINLAEVVDRTL
                     NLHAYSLRKNNITVDFLRAEGLPYAMGDPHQLMQVFLNLILNAEQAIREVRDRGTLRI
                     RLGKADNSVWVSFQDDGPGIAKDVLPSIFDPFYTTKRPGRGTGLGLSICKSVMKEHNG
                     SIEAANAPGGGAVFTVTLPVTG"
BASE COUNT         1094 a         1580 c         1569 g         1049 t
ORIGIN      
        1 ccgtggaagc acccggcttg ccgaggccaa agaaaatgcg gttcgcctga tgaccgttca
       61 cggagccaaa ggcctggaat tcccccacgt tttcatcctt cgcgcgaact caaattcctt
      121 cccttgttct taccgcgaga ccctggtcgc cttccccagg gaactgcgcg atgaagactc
      181 gatcaccgcg gatgacgacc gcgagcttca gaagcaggaa gaacgccggc tgttttacgt
      241 ggcgatgact cgcgcccgcg actccctgcg gatctatgcc aggcagggaa gaggcgccaa
      301 caagaatcct gacggctaca tgcgtgaact gatcgagaac cgaggcctga cccgctggtt
      361 caagtcgatt cacgcgagtg gcgcacaggc gaatcttgac atttttgctg aggccttgcc
      421 cgcctaccca gaccagtcgc gtacgacttt atggtttgaa ctgccggtgc gcgacggctt
      481 gtatacgcgg ctcagcgctt cggccataga cacttaccag cgctgcggat tgcagttcaa
      541 gctcgaccgc gactggcaca ttcccgccaa gcccgccgcg gccatgcaat acggcgccag
      601 catgcaccgg gtcctcaaga cgtatttcga ttccgtgcgc ctcggccgtc ccaagagcga
      661 cgaggagaca atcgaacttt tccgccaaga tctggcggaa gccggcatcg ctgaaaagta
      721 ccagcacgag ctctacgaaa agcagggcat caaccaacta cgcgagtttc ttacatcgtt
      781 acgatcgctc cctgctctcc aggttctgca tacagagcag ccctttgaaa ttcgcatggg
      841 cccggtgacg gtcatcggcc gcatcgaccg cattgaccgt cgcgccgacg gcagcgttgc
      901 gattgtggac tacaaaaccg gcaaggcgcg tcctcaggaa gacgctgacg acagccttca
      961 gttgtctctc tatgcgattg cggcgcagga gaaatgggga tacaaggtcg gcgagctgat
     1021 tttcttcaac cttgatggga atgttccggt gatcaccacg cgctgcgaag ccgatttgct
     1081 cagcgcccgc gagcgcgtgg aggctgcagc caagggaatt gccgccggcg tcttccatgc
     1141 gaagcccggc aaccattgca acttctgcgc ctacaggagc ctgtgtccgg aaaaagagaa
     1201 gcggatccca cgaacggtcg caatcggcac ggcgcaaacg aactgacggg agcgagtcgt
     1261 gttctcgtgc acaaaaacct taggggacgc attccgcgtc ccctgtttga cttcgtactg
     1321 aaccttctta gtgtttcttt ttcttctttg ccttcttctt ggttgccatt gttcctattc
     1381 tcccttccat tcttcatgga aaatgtgcag tggtcttcta ctgcaactag ttgatgtata
     1441 gagttaatga aaaatgaagt caagagaaaa atgatgatga aggagtgtga agtggtagca
     1501 caaaagtcac gacgagcgaa gttggaatgc aaaagtcaac aagattctga tcggaggatg
     1561 aaagcggcag ctctcgtctg ttgccgtgcc agcggaattt cgttcctgtg accgaggaat
     1621 gagttactgt gtgagcctga ctgtcggtga atgcgcgctc acgaccgcgc agccatttcc
     1681 tttcccgcgc atctataatg tgaattgcga aatcatcgcg ggagcttgat tcatggcctc
     1741 ttttgacgat gtagtcatca tctccggttg ccgcacaccc gtgggcaaat ttcaaggaag
     1801 cctttctgaa ttcagcgcgc cgcaactggg cgcgatggtg gtgcgcgaag cggtgaagcg
     1861 cgcgggagtc gatccggcgc aagtcgacga gtgcatcatg ggcaatgttg tttccgcggg
     1921 gctcggccaa aatcctgccc gccaggccgc gatcttcggc ggactcgctc cgaccacagg
     1981 tgccatgaca attaacaagg tttgcgggtc cggattgaaa tcagtcgccc ttgccgcgca
     2041 agcgatccag accggaaaca gttcgatcgt ggtcgccggc ggaatggaat ctatgaccaa
     2101 tgcgccgtat cttctgccgc aagcgcgcaa gggatatcga ctgggcaacg cgcaggttgt
     2161 cgattccatg gtgcatgacg gactgtggga catctacaac gactaccaca tgggcattac
     2221 gggcgagaac gttgccgaga agtacggcat ctcgcgcgaa gagcaggatg agtttgccct
     2281 caactcccac cgcaaggctc tcgccgccat caaggagtgc cgtttcaagt cacagatcgt
     2341 gcccgtcgag attcccgcga agaagaaagg cgcgccaccg accatcttcg acaaggacga
     2401 gtctccgcgc gaggacacca caatcgaggt gctgcgtgcg ctgaagccgg cgttcaagaa
     2461 ggatggaacc gtgacggccg gcaatgctcc aggagtcaac gacggcgccg cggcagtggt
     2521 cgtcacgagc gcgcagcggg cgaaagagct cggcgcgaag ccaatggtcc ggatcgtggc
     2581 ccaggcgacc agcggggtcg agccgaagtg ggtcatgatg gctcccgttg gcgccgtgcg
     2641 gaaaatctgg gaaaagacgg gatggaagaa tgaggacgtc gatctctacg agctcaacga
     2701 ggcattctcg gttcaggcgt tgggcgtgat gcgcgaactc ggcctgaatc ccgacaaggt
     2761 gaacgtgaac ggcggcgcgg tggcgatcgg acacccgatc ggcgccagtg gcgcgcgggt
     2821 tctggtcacg ctgatctacg aaatgatccg cagagatgta aggcggggga tcgcggccct
     2881 gtgcctcggc ggagggaatg ccgtggcgat ggcggtggag cgctgagagt tcaccctgtt
     2941 accggcagcg tcaccgtgaa cactgcgccg ccaccaggtg cattcgcggc ttcgatgctg
     3001 ccgttgtgct ctttcatcac cgatttgcag atgctcagcc ccagtcccgt gccgcgcccc
     3061 ggacgcttgg tcgtataaaa cggatcgaag atgctgggta gaacgtcttt cgcgatcccg
     3121 ggcccgtcgt cctgaaaact gacccagacc gagttgtcgg ctttcccgag acgaatcctc
     3181 agcgttcctc tgtcgcgcac ctcgcggatg gcctgctcgg cattcaaaat gagattgagg
     3241 aaaacctgca tcagctggtg cgggtctccc atcgcatagg gcaacccctc cgcgcgcaga
     3301 aagtcgaccg taatgttgtt cttgcgcagg gaataggcgt gcaggttcag agtccggtcg
     3361 acgacctccg ccaggttgat gcggctcttg cccggagccg gcggccgcga gaaatatgtc
     3421 aaattctgta cgatctccgc cgcccggcgg gcctgctgct gcagcatggc aagctgcttg
     3481 cgcgtggttt cgttggtttc gctgtcctgc agcagttcgc tgactcccag aatgctggtc
     3541 agcgggttat ttagttcgtg ggcaacgccg ccgatcatgg cacccatggc ggccagccgt
     3601 tcgctctgca ccacctgctg ctcgagcttt ctttcgatgg tgatgtcgcg cacggaaaag
     3661 atgacgccgc tgatcctcgc gtccgcggcg aagagctggc tgccggcggc gcgcatggtg
     3721 cgccagcttc catcgtgatg tcgggccgca tactcggcgg atccgaaggc ctccttgccc
     3781 gacacaatag tctgataaag caacgacaat tccgacgaag aggcttccag gtcggtgatc
     3841 tttttcccca gcatgtcttc cggcttgcac cccagcagat cgcccacacg ggagctcacg
     3901 aacgtgtagc gcccctccag atccacgacg agaatcagat ccgggaagct ttccagcagc
     3961 tgccggcgaa attcttcctg ctgcgccagt tgccgctcca tggcgcgttt ttcggtgaca
     4021 tcgaccaagg ttccctgata gcggatgatg ttgccggccg catcccacac ggcgcgggac
     4081 gaatcgagaa acaccgccgc cgatccatct ttgcggcgca gcttgatctc gcgcgtgcgc
     4141 acgccgccac ggtcgtcaac cgcccgaccc agcaccggat cctgaccggg atcgaaattc
     4201 agcgccgccg gctcgaccgc cagcagatcg cgcttctcct tgtatcccag catgctgacc
     4261 aacgcggggt tggcgtcgag caacttgccc tcgggggaac tgaaatacgc accctcctgc
     4321 agggtctcga acagttcggt gaagcgcaat tccttctcgc gctgttcggt tacatcgcgg
     4381 ccgagcatgc ttgccccgac cacctcgtcg cctttgacga tggcattcat cacgcagtcg
     4441 aagtacagag gccgcgaact gttcttcagc cggatctgca ccattcccgc ccagcgcttt
     4501 ttttccagaa aacgccccag tccgcgttcc atctcctcgc gggttggttc gtcgatgaag
     4561 tcgtagatct tatgccccac cagctccgag taatgcagcc cggccatctc agccacacgc
     4621 cggttcacgg tgcgcaatgt tccatcgaga gatacggcac aggcgggatc atcaaaggag
     4681 tcgatgagct ccttgaaact ccgctgcgag cgcatgatga cctggctgag ctgatccagt
     4741 tgttcttcag agggcgccgc gccaacgtgt tcctggagat tttgcagcag gcctttgagt
     4801 tcggtgacct cgcgacgccg cccgtaggca tagaccgcaa gcaggaccga gagcaccaaa
     4861 acaccgagcg caacggggcc cagatgatac gggagatcct gcagccgctc ccaggcgagt
     4921 gccgcaaagc ccactgccag cagaaccatg aagaaaagca gccagcgcca aagctgggat
     4981 tcctcctgtt cgagcttccc gggagggcgg gctggcggcg gggtttgagc agcaaatggg
     5041 gtcattcggg accgagcgga tcttaggcta gctcatccta tcgtgcgcga ttgggcggaa
     5101 caatatcgtt ttctggaaca tgatccccgg cggtaatcag gggcaatcag gcgcggggcg
     5161 agtgcccgct cggaccggct gaatagatcg ctggccgggc cggcgacgtt ttccacagta
     5221 cttcggtgac atccgtcaca gccagcattg caccagaatc cgtaaggtga acatctggga
     5281 agtcaggccg ac
//
LOCUS       DASPUL010000631         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2043848, whole genome shotgun sequence.
ACCESSION   DASPUL010000631 DASPUL010000000
VERSION     DASPUL010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2043848"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VEJ00_14570"
     CDS             <1..884
                     /locus_tag="VEJ00_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXX02437.1"
                     /translation="PQRRAQHDLSSLRALINCSEPVRATSMREFQSAFSAAGLQSHVL
                     QSSYAMAENVFAVSQSDITRRDGPACIWADGQQFRSAHRIVSVAEGTPGAVVFMSSGR
                     LLPNHEVRTASDSGEGLNEGSVGEILVKSDCLFSGYYNRPDLTTEAIVDGWYHTGDLG
                     FMLDGELYVVGRKKDLLIIGGENIYPQDIEELVAGHTAVHDGRAIAMGVYNPDLGTEE
                     IVVVAELESENLLARSGEIEQEIRARVVAGMGVVVKMVFLKPPKWIVKSTAGKAARSA
                     TREKLLREHPELNPESA"
     gene            949..1179
                     /locus_tag="VEJ00_14575"
     CDS             949..1179
                     /locus_tag="VEJ00_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HXX02438.1"
                     /translation="MIEHVTGLIRKPGVNIDENTPLISSGLIDSMALVDLLVKLEDLT
                     HMRIPAGKIQPKDLDTVAKMFATAQRVGRPRR"
     gene            1581..>3869
                     /locus_tag="VEJ00_14580"
     CDS             1581..>3869
                     /locus_tag="VEJ00_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02439.1"
                     /translation="MLLSVVSHLVNPGLAQGQAAREQPAAVLAASGTQSGRLTRIYPM
                     RDGNRASVKPGRFHTANGDATASAATTGGPDLQYYGGPVISNVQVVVVYWGNSVSSVA
                     TSGIPGFYTAVTNSNWMDLLSEYNTAGVTPAGGGGAGDQAIGRGFVSGVYTITPSAAN
                     SGSTVDDTQIQAEIVAQINAGHLPAPATDSTGAITTLYMIYFPPGVTITLQGAASCAQ
                     FCAYHGTTNFNKLDLPYGVLPDLGPGTACSTGCAGGTEFEDLTTVSSHEMAESITDAG
                     VGVATALGPPLAWYDSTFGEIGDICNASGVPANQAVLPGSGYTVQKLWSNLQQDCVSA
                     PPVFTVSAPASVSSGTPFTITLSVESSTGVALSPPYTGTVEFTSSDSAALLPGNYTFT
                     TADSNSHTFANAATLKTAGAQTITATDTHSGGFTGSAQMNVSVHTSPYFSLAVPSAAI
                     LGTPFSFTVTALTPSGSLNAAYSGTVHFTSSDPQALLPANATLTNGTGTFSTTLHTSG
                     SQTITATDTVTATITGSSPGIAASGAATHFAVVAPAAATIGAAFTFTVTAEDQSNNPA
                     PTYSGTVHFSSSDLQALLPANATLSNGTGTFSATLNTKGTQTITATDTANGTISGTSG
                     SITTSAAATHFAVTAPASATAGTAFNFTVTALDSANNTAATYAGIVTFTSSDGLAVLP
                     GNTTLTNGTGTFTAVLKTSGAQTITATDTATTSITGVSGSISVIVAPPAAMPVFSPNP
                     GTYNTPQTVTITDASSGAAIYYT"
BASE COUNT          767 a         1249 c         1171 g          682 t
ORIGIN      
        1 cgccgcagcg aagagcgcag cacgatcttt cgtcgctgcg cgcgctgatc aactgctcgg
       61 agccggtgcg agccaccagc atgcgagagt ttcagagcgc gttctctgcc gcgggattgc
      121 aatcgcacgt attgcaatcc tcttacgcca tggccgaaaa cgtttttgca gtcagccaat
      181 cggatatcac ccggcgcgat gggcctgctt gcatctgggc agatggccag caatttcgca
      241 gcgcgcaccg catcgtgtcc gtggccgagg gaactccggg agcggtggtc tttatgtcat
      301 ccggccgtct gttgcccaat cacgaagtgc gcactgcgtc cgactctggc gaggggctca
      361 acgaaggaag cgtgggtgag attctggtca agagtgactg cctgttttcc ggctactata
      421 accggccgga cctgacgacg gaggcaattg tcgatggctg gtatcacacc ggcgaccttg
      481 gcttcatgct cgatggcgaa ctctacgtcg tcggccgcaa gaaagacctg ctgatcatcg
      541 gcggcgaaaa catttatccg caggacatag aagaactcgt cgccggccat acggcggttc
      601 acgacggccg cgcgatagcc atgggagtgt acaatcccga tctcgggacc gaagagattg
      661 tggtcgtggc ggaattggag agcgagaatc ttctcgcacg gtcaggcgag atcgagcagg
      721 agattcgcgc ccgcgtcgtt gccggtatgg gagtggtggt aaagatggtc tttctcaagc
      781 cgccgaaatg gattgtgaag agcaccgcgg ggaaggcggc gcgatccgcc acacgcgaga
      841 aattgttgcg agagcatccg gaactcaacc ccgagagcgc ctgacccgcg caaggttgac
      901 tgcagatcgg agagtagcac cgtcgccatg accccagagg cgcagcagat gatcgaacac
      961 gtcaccgggc tgatccgcaa gccgggcgtg aacatcgacg agaacacgcc gctgatttct
     1021 tccgggctga tcgactcgat ggcgctggtc gacctgctgg tgaagcttga agacctcacc
     1081 cacatgcgca tcccggcggg aaagatacag ccgaaggacc tggacacggt ggccaagatg
     1141 ttcgccaccg cccaacgggt aggaagacct cggcggtaga tccgccgcat gcctcaatca
     1201 ggattaactt tcctggtgcc agacccaaat taggaaccgg gcgaaacgaa tttctcacat
     1261 tcgttggtga cagcgcgccc gaaatcctcc ataatccgct aaaggcttcg gtaatcaccc
     1321 acctccgagg ctatggggct tatcaatcgc aacgactact ttcctggtgc accgggcagg
     1381 cacggctgta ttcggccggt gtcggggcgg caagaggact gttaagaaat cttaggggtg
     1441 gttggttgta tgcgcgcgtc ttcattgctt ttgtccggtt cccgggaggg taagctcgta
     1501 tgaagtggcg cacgcctcaa ggttgccgca gtgtgtcgtc aatatttggc agtagcccag
     1561 ttcggcgaat ctcgccggta gtgctgctat ccgtagtgtc ccatcttgtc aacccggggc
     1621 tcgcccaggg gcaggcagcg cgggagcaac ccgcggcggt attagcggcg agtggcactc
     1681 aaagcgggcg cctgacgcgc atctacccca tgcgcgacgg gaatcgcgcc agcgtcaagc
     1741 cggggaggtt tcatacggcc aacggcgatg cgacggccag cgccgcgact accggaggcc
     1801 cagatctcca gtattacggc ggcccggtga tttcgaatgt gcaagttgtg gttgtctact
     1861 ggggaaatag cgtcagctcg gtcgcgacga gcgggattcc gggcttttac accgccgtca
     1921 ccaacagcaa ctggatggac ttgctctcgg aatacaacac cgccggagta actccggcgg
     1981 gcgggggcgg ggcgggcgac caggcgatcg gacgcggctt cgtcagcggc gtctatacca
     2041 tcacgccgtc cgcggccaac tccggcagca ctgtcgacga tacgcagatt caggcggaaa
     2101 tcgtggcgca gatcaatgcg ggacacctgc ccgcgccggc taccgacagc actggcgcca
     2161 tcacgacgct ctacatgatc tatttcccgc cgggagtcac gattaccctg cagggcgctg
     2221 cgagctgcgc gcagttctgc gcctaccacg gcacaacgaa tttcaacaag cttgatctgc
     2281 cttacggcgt gctgcctgac ctgggcccgg ggacggcatg ctcgacgggc tgcgccggcg
     2341 gcactgagtt tgaagatctc acgacggttt cgtcgcacga gatggcggag tccatcaccg
     2401 acgcgggggt cggcgtggcg acggctctcg ggccgccgtt agcctggtac gacagcacgt
     2461 tcggcgaaat cggtgatatc tgcaatgcca gcggtgttcc cgccaatcag gcggtgctgc
     2521 cgggaagcgg ctacaccgtg cagaagctgt ggtccaactt gcagcaggac tgcgtcagcg
     2581 ccccgccggt gttcaccgtg tcggcgccgg cgagcgtgtc gtctggaact ccgttcacga
     2641 tcacgctgtc ggtcgaaagc agcaccggcg tggcgctcag cccgccttac accggcacgg
     2701 tcgagttcac cagcagcgac agcgcggccc tgttgccggg aaactatacg ttcacaacgg
     2761 cggattccaa ctcgcacact ttcgcgaatg ctgcgacctt gaagactgcg ggcgcacaga
     2821 cgatcaccgc gactgacacg cactccggcg gcttcaccgg atcggcgcag atgaatgtca
     2881 gtgtccacac ttcgccttac ttttccctgg ccgtaccctc ggcggcgatc ctgggcacgc
     2941 cgttcagttt caccgttact gcgctgacgc cctcgggcag ccttaacgcg gcctacagcg
     3001 gaacagtgca cttcaccagc tcagacccgc aggcgctttt gcccgccaac gccaccctga
     3061 ccaatggcac gggaaccttc agcaccacgc tgcacacgtc cggcagccag accatcactg
     3121 cgaccgacac ggtgacggcc acgatcaccg gctcttcgcc cggcatcgcg gcttccggcg
     3181 cggcgacgca ctttgccgta gtggcgccgg ctgcggccac catcggcgcg gcgttcactt
     3241 tcaccgtaac cgccgaagat cagtcgaaca accccgcgcc cacctacagc ggcacggtgc
     3301 atttctcgag ctcggacctg caagcgctct tgcccgccaa cgcaaccctg agcaacggaa
     3361 cgggcacgtt cagcgcgacc ctgaacacca aaggaacgca aaccatcact gcgaccgaca
     3421 ccgcgaatgg cacgatcagc gggacttcgg ggagcatcac gacttcggcc gcagccacgc
     3481 actttgcggt tacggcgccg gcttcagcga cggccggtac cgccttcaac ttcaccgtga
     3541 ccgccctcga ttccgcaaac aacaccgcgg cgacctacgc cggcatcgtg actttcacca
     3601 gctccgacgg gctagcggtg ctgccgggca acactacgct gaccaatgga accggcacgt
     3661 tcaccgcggt cctcaaaact tccggcgcgc agaccattac cgcgaccgat acggccacca
     3721 cttcgattac cggcgtctcg ggcagcatct cggtgatcgt cgcaccgccc gcggccatgc
     3781 ccgtcttctc gccgaacccg ggaacctaca acacgccgca aaccgtgacc atcaccgacg
     3841 cgtcgtccgg agcggccatc tactacacc
//
LOCUS       DASPUL010000632         5726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1767843, whole genome shotgun sequence.
ACCESSION   DASPUL010000632 DASPUL010000000
VERSION     DASPUL010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5726)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5726
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1767843"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1628
                     /locus_tag="VEJ00_14585"
     CDS             <1..1628
                     /locus_tag="VEJ00_14585"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXX02440.1"
                     /translation="VVCHFTALALLFAFTRIFALQPHAVPALPQAFDHSQVIYYEASE
                     YLPPLDTRSADDPPPQPADPEFAKQPIISVPPEADNHSQTIVTPPKLKIKHDVPLPNI
                     VAWSDKVVKPQLAIPAAPAIPAAEVTRIAPRVETSVVTPPPDSAHLAHRRDQPNLQAS
                     VVAPPPELRAAGAGPALPGPQTAVIAPPPSVDAAALAVQTVGDLNIGHSAVIAPAPEL
                     AVAEQRALPAGRNSAQPGLAAQIVPPPPAIASSGASGSPGRAVALNLHPDVGAPPDVP
                     AGNRRGTFAATPEGHPGASGSPGSSAGSSASGTGGRGSGNGNGNGSESSPKGTHDLPA
                     GLYVGNVASTHPVAPLAGDPAPRMVAAASPAPAPGMRPHVGGASTIHLQPGNPAALSA
                     PERAIFGASRFYSLTLSMPNLNSGGGSWIIRFAELKHETEKHGADYAAPDAPEGDLTP
                     PTVTRKVDPAYPLQLMRENVSGTVILYAIIHADGTVGSVRVVRSVDDRLDRFAAEAIS
                     QWKFDPATKNGAPVDVEATFQIPFRPTQIGTSF"
     gene            complement(1776..4013)
                     /locus_tag="VEJ00_14590"
     CDS             complement(1776..4013)
                     /locus_tag="VEJ00_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02441.1"
                     /translation="MPRIPIFRLGGAPEKPALPSLAASTPLISLEGLSVGVDNLRHDV
                     LLSQRFVEVARAHIARLIARHGDLEGLLSAEAPVASQGPSWMRNLAGKQARPKADPND
                     WKSLLTELLVNSLNRAKKEYKLGVDLLARLAVTKFLRTEMNQQFSQVVERCRVLLKSY
                     DNMRQSKAHEYRERVATFQVRKKIILRKTGQDIFETLREVEKSTLARTRRSLFGEETS
                     ATTYFTYPLFLNRLLFSEDGRDDFLCAEHYVMLGNWDRDADRYGRMREIVTGFLRSLY
                     GEETTSETIDAWMNVRENARLLVGTGTPEESDEGVAQQERLAAWIRLLEDERVMENVI
                     ASYYVVPLLSEYAPRINPQQLKNALIDRTECDRVERMIQEGGKLTPNSLYAAVAKVAS
                     CRGAERSKVAARFLGDFFHYHRDLRRLEVLNTALDSVNLATNQRLQELSRVNGTLYEF
                     VLPEEQAQGDSDRVLRHVVLKADVRDSTRLTRMMMEKGLNPASYFSLNFYDPVNKLLE
                     KYGAKKVFLEGDAIILAILEREGEPGLSVSRMCVLAREVIEIVRGYNELMQRSAMPQL
                     ELGVGITLQESAPLYLMDGEHQIMISEALNESDRMSSCNKRARRVMEPIGGPFHVYAF
                     QAAEMDADGNPEDVVLSFNLGGIRMNEAAFRQLQQEITLEPLKVRLPAALASSDHGEY
                     RLFSATVPVDHEIFRKIVVRESRIPSIDPADFSVTGWTERLYYEVCTDPAIYAALEKR
                     KGAAR"
     gene            complement(4189..>5726)
                     /locus_tag="VEJ00_14595"
     CDS             complement(4189..>5726)
                     /locus_tag="VEJ00_14595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX02442.1"
                     /translation="LQPESVRTPAIRPWAVFGVAVLVLAAAALSYFSWVRWRGRASLS
                     ANRQILAVLPFENLTGDPGQEYFSDGITEEMITALGGLDPQHLGVIGRTSVMLYKRNP
                     KALDQVGRELGVQYVLEGSLQRDASRLRVTAQLIQVRDQTHLWAHTYDREVSDVLALQ
                     QEVTQEIADEIELVLGEPGAPNARVRQVAASTGSSEAYDLYLRGRFFWNKRTTDGFER
                     AAEYFQQAIDKDPNYARAYAGLADTFGLMSTWRMAPQNEFMPKARAAALNALKLDPTL
                     AEAHASLALIAENYDFDWRTAEKEFQRAIQLDPEYPTAHQWYAECLGWQGRFDEAFAE
                     SERARQLDPMSLIIARDYVSLLFLSRDYDHAISHDRSLLDMDPSFNNARSILIGSYLQ
                     QGKFRQAQSEIETGYRSPDPLSIWVARAELYGRWGRTAEAEKALAQAEGYAGPSRLDM
                     PGALFHAYVATGRKDQALAILEKAYSEHSNMLTAIKVDPVFDPLRGDPRFQDLMRHVG
                     LAH"
BASE COUNT         1059 a         1972 c         1584 g         1111 t
ORIGIN      
        1 cggtggtctg tcactttacg gcgttggcat tgctgttcgc cttcacgcga atctttgcgc
       61 tccagccgca cgcggttccc gcgctgccgc aggctttcga tcactcccag gtcatctact
      121 acgaagcctc ggagtatttg cctccgcttg atacgcgcag cgccgacgat cctccgcccc
      181 agcccgccga ccccgaattc gcgaagcagc cgatcatttc cgtgccgccc gaagccgata
      241 atcactccca gacgatcgtc acgccaccca agctcaagat caaacacgac gtcccactgc
      301 cgaacatcgt ggcgtggtcg gataaagtcg taaaaccgca gcttgccatc cccgctgctc
      361 ctgccatccc ggccgcggaa gtcacgcgca tcgctccccg cgtggagacc tcagtcgtca
      421 cgccgcctcc cgactccgcc caccttgccc atcgccgcga tcagccgaac ctccaggcgt
      481 cggtggtcgc tcctcctcca gaactgcggg ctgccggtgc aggtccggcg ttgccgggcc
      541 cgcagactgc cgtcatcgca ccgccgccca gcgtggatgc cgccgcgctc gccgtgcaaa
      601 ctgtgggaga tctgaacatc ggacacagcg cggtcatcgc gcccgcgccc gagctggcgg
      661 ttgccgaaca gcgcgctctg cccgccggac ggaactccgc ccagcccggc ctcgccgccc
      721 agatcgttcc gcctcctcct gccatcgcct cctcaggcgc ctccggctct cccggccgcg
      781 ccgtcgcgct gaacctgcat ccggatgtag gcgcgccgcc cgacgtgcct gccgggaatc
      841 gtcgcggcac tttcgccgca acgccggaag gacacccggg cgcctccggc tcccccggtt
      901 cttccgctgg aagctcggct tcgggaacag gcggtcgcgg cagcggaaat ggaaacggaa
      961 atggcagcga atcaagcccc aagggcacgc atgatctgcc cgccggtctc tacgtgggaa
     1021 atgtggcctc tactcacccg gtcgcgccgc ttgcgggcga tcccgctccg cgaatggtcg
     1081 ccgctgccag tccggctccc gcgcccggca tgcgcccgca cgttggcggc gcttccacga
     1141 ttcatctcca gcccggaaac cccgctgctc tgtcagcccc cgagcgcgcc atcttcggcg
     1201 ccagccgctt ttactccctc actctgagca tgccgaatct gaattccggc ggtggcagct
     1261 ggatcatccg cttcgccgag ctcaagcatg aaacggagaa acacggcgcc gattatgccg
     1321 cgcccgacgc gcctgaagga gatctcaccc cacccaccgt cacgcgcaag gtcgatccgg
     1381 cttacccgct ccagttgatg cgcgaaaacg tctccggcac cgtgattctc tacgccatca
     1441 tccatgccga cggcacggtc ggcagcgtgc gcgtcgtgcg cagcgtcgac gatcgccttg
     1501 accgcttcgc cgccgaagcc atctcgcagt ggaagttcga tcccgccaca aaaaacggcg
     1561 cccctgtcga cgtcgaagcg accttccaga tccccttccg ccccacgcag atcggaacga
     1621 gcttctaaga agcttgagag ttgccttgat gagtctgacc ggtttgattg gtttgaaagg
     1681 acgcggcttc aagccgcatt ggccaggcca agcggaagca agccctaaaa tccaacactg
     1741 atccctgacc gctgacccct gatccctggc ccgccctacc tcgcagcccc ttttcgcttc
     1801 tccagcgccg cgtaaatcgc cggatcggtg cagacttcgt agtacagacg ctcggtccat
     1861 cccgtgacgg agaagtccgc cgggtcaatg ctgggaatgc ggctctcgcg cacgacaatt
     1921 ttgcggaaaa tctcgtgatc aaccggtaca gtcgcactga acaggcggta ctccccgtga
     1981 tcggatgagg ccagtgccgc cggcagccgc accttgagcg gctcaagtgt aatttcctgc
     2041 tgtagctggc ggaaggcggc ttcgttcatg cgaatcccgc ccaggttgaa gctcaaaacc
     2101 acgtcttccg gattcccatc cgcgtccatc tccgccgcct gaaaagcgta gacgtgaaat
     2161 ggcccgccaa tcggctccat caccctgcgc gcccgcttgt tgcacgagga catgcggtcg
     2221 ctctcattga gtgcttctga aatcatgatc tggtgctcgc cgtccatcag gtagagcggc
     2281 gccgactcct gcagcgtgat gcccaccccc agctcgagtt gcggcatcgc cgaacgctgc
     2341 atcagctcgt tatatccgcg cacgatttcg atgacttccc gagccagcac gcacatccgg
     2401 ctcaccgaca atcccggctc gccctcgcgc tccagaatcg ccagaatgat ggcgtcgcct
     2461 tccaggaaca ctttcttggc gccatacttt tcgagcagct tgttcaccgg atcgtagaaa
     2521 ttcaggctga aatacgacgc cgggttcagc cccttctcca tcatcatccg cgtcagccgc
     2581 gtcgagtcgc gcacgtcagc cttgagcacc acgtgtcgca acacccgatc tgaatccccc
     2641 tgcgcctgct cctcgggcaa cacaaattcg tagagcgtcc cattgacccg cgacagttcc
     2701 tgcagacgct ggttggtcgc caggttcacc gaatcgagtg cggtgttcag cacctccaac
     2761 cgccgcaagt cgcgatgata gtgaaagaaa tctcccagga accgtgccgc gactttggat
     2821 cgttccgcgc cgcggcagct cgccaccttc gccacggctg catacagact gtttggcgtc
     2881 agcttgccgc cctcctggat catgcgctcg acgcggtcgc actccgtgcg gtcgatcagc
     2941 gcgttcttca gctgctgcgg attgatgcgc ggcgcgtact cgctgagcag cggcaccacg
     3001 tagtacgacg cgatcacgtt ctccatgacc cgctcgtctt cgagcagtcg gatccacgcg
     3061 gccagccgct cctgctgcgc caccccctcg tcggattcct ccggtgtccc cgtccccacc
     3121 agcaggcgcg cgttttcccg cacgttcatc cacgcgtcga tcgtctcgct cgtcgtttcc
     3181 tcgccgtaca acgatcgcag gaatcctgtg acgatctcgc gcatccgtcc atagcggtcc
     3241 gcgtcgcgat cccagttgcc cagcatcacg tagtgctcgg cgcacaggaa atcgtctcgc
     3301 ccgtcttcgg aaaacagcag ccggttcaga aacagtggat aggtgaaata cgtggtcgcg
     3361 ctggtttctt ccccgaacaa cgaacgtcgc gtgcgtgcca gcgtgctttt ttccacttcg
     3421 cgcagcgttt cgaaaatatc ctgccccgtc ttgcgcaaga tgatcttctt tcgcacctgg
     3481 aacgtggcca cgcgctcgcg atactcgtgc gccttcgact ggcgcatgtt gtcgtagctc
     3541 ttaagcagta cccggcagcg ctccaccacc tgcgaaaatt gctggttcat ctcggtgcgc
     3601 aggaatttcg tcaccgccag ccgggccaaa aggtcgacgc ccagtttgta ttccttcttg
     3661 gcgcgattca gagagttgac gagcaactcg gtcagcaaag atttccagtc gttgggatcg
     3721 gcctttggcc tggcctgctt gcctgccaga ttccgcatcc agctgggacc ctggctcgcg
     3781 accggtgcct cggccgagag caagccctcc agatcgccgt gacgggcgat cagtcgcgca
     3841 atgtgcgccc gcgccacctc cacgaaccgc tggctcagca gcacgtcatg gcgcaggtta
     3901 tcgacgccca ccgacaatcc ctcgagcgaa atcagcggcg tcgacgcagc aagactcggc
     3961 agcgcaggct tctccggcgc tccgcccaac cgaaagatag gaattctcgg catcccaggc
     4021 aggtttcagt acgcaagttg ctgattacac acctacatat cgaggctagc atggggatcg
     4081 cgagggctaa agttacctag gttaccggcc ctccggcgtg ccgaaccggg ctcggcaaac
     4141 cccgagttca ctgtgaatct agcgtgttaa gggaggaaac gatatggttc aatgcgccaa
     4201 tcctacgtgg cgcatcaagt cctgaaagcg cgggtcgccc cgcaagggat cgaacacggg
     4261 atcaacctta atcgccgtca gcatattgga gtgttcggaa taagcctttt ccaggatggc
     4321 caaagcctga tccttgcggc ccgtggcgac ataagcatgg aacagggctc ccggcatgtc
     4381 caggcgcgat ggaccagcgt atccttcggc ctgcgccagg gctttctcag cttcagccgt
     4441 tcgaccccat cgtccgtaga gttctgctct tgccacccag atcgaaagcg gatccggcga
     4501 cctgtaccca gtctcgattt cactctgagc ctgtctgaac ttgccttgct gcaggtaaga
     4561 gccgatcagg attgagcgag cattgttaaa gctcgggtcc atgtccaaca aggagcgatc
     4621 gtgcgagatc gcatgatcgt agtcccgcga gaggaacagg aggctcacgt aatcgcgggc
     4681 gataattaga gacattggat ccagctgacg cgcccgctcg ctctccgcga aagcttcgtc
     4741 gaaacgccct tgccagccca ggcattccgc ataccactgg tgagcggtcg gatattcggg
     4801 atccagctgg atggcgcgtt gaaattcctt ctcggctgtt cgccagtcaa agtcataatt
     4861 ctcggcgatc aacgccagcg acgcgtgggc ttcagccagg gttgggtcga gcttcagggc
     4921 gttcaacgcc gctgctcgcg ctttcggcat gaactcgttc tgaggagcca tgcgccacgt
     4981 gctcatcaag ccaaacgtat cggccagacc ggcgtaggcg cgggcatagt tcggatcctt
     5041 gtcgatagcc tgctgaaagt attcagcggc gcgctcgaaa ccgtcggttg ttctcttgtt
     5101 ccagaaaaag cggccccgga gatagagatc gtaggcttcg gatgatccgg tggaagcggc
     5161 cacttgtcgg accctggcgt tcggagcccc cggctcgccc agcacgagct caatttcatc
     5221 ggcgatttcc tgtgtcactt cctgctgcag ggccaataca tcgctcacct cgcgatcgta
     5281 ggtgtgcgcc cagagatggg tctggtctct tacctgaatc aactgcgccg tcacccgcaa
     5341 gcggctggca tcacgctgga ggcttccctc gagcacgtac tgtaccccca gttcccgccc
     5401 cacctgatcc aacgctttcg ggttgcgctt gtacagcatg accgaagtgc ggccaatcac
     5461 tccgagatgt tgcgggtcga gccccccaag tgcggtgatc atctcctcgg taatcccgtc
     5521 gctaaagtat tcctgtcccg gatcgcccgt gagattctcg aaaggcagca cggccagtat
     5581 ctggcgattg gcggacagcg atgctcggcc ccgccatcgc acccacgaaa agtagctgag
     5641 tgccgcggct gcgagcacca agacagcaac tccaaaaaca gcccacggac gaatcgctgg
     5701 cgttctgacc gactcgggct gcagcg
//
LOCUS       DASPUL010000633         9285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_673734, whole genome shotgun sequence.
ACCESSION   DASPUL010000633 DASPUL010000000
VERSION     DASPUL010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9285)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673734"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VEJ00_14600"
     CDS             complement(<1..1589)
                     /locus_tag="VEJ00_14600"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HXX02443.1"
                     /translation="MISRCLSLTLGLLSLALRIYSQTAAASQAAAKPDYSQQAYVIEE
                     MSTNITFDNEGNRTREQITRVRVQTDAGVQEWGLLSLPYQSAAETVEVNYVRVRKSDN
                     TTVTTPSDNIQDLDAEITRAAPFYSDLREKHVAVKGLGKGDVLEYQAHWRPTKPLIPG
                     QFWLEYSFHHDGIVLNERLEVRVPASRAVKYKGPQATQVVNTQGDSRVYAWTYTNLEG
                     KPEVESEKQKRDAALGRLPAPDVQLSSFQSWDEVGSWYWNLQKDRIEPSPAIRAKAGE
                     LTKGTTDDAAKLQALYSFVSTQYRYIGIAFGIGRYQPHAADDVLSNNYGDCKDKHTLL
                     ASLLQASGLTIYPALISATHALDPDVPSPGQFDHVIGYLPQGKTALWLDTTPEVSPPG
                     FLMLPLRDKPALVMAGEKSFTLTTTPADPPFPSAQTFKIEGKLNDDATFQAHIEDTSR
                     GDAEIVLRSAFRRVPQPQWKDLVQQISYGLGFAGLVNEVSASSPEAVTEPFHFSYSYT
                     RKDYPDWSNRQFTVPGLPFYMP"
     gene            complement(1726..3300)
                     /locus_tag="VEJ00_14605"
     CDS             complement(1726..3300)
                     /locus_tag="VEJ00_14605"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02444.1"
                     /translation="MADYIYTMELRLTPDQLKGVGLVQDVARAAGMNLYLTGGAIRDI
                     ISGFSIRDLDFTVQGNPLKLQKDFEKAGARITAVDEDARTIFISLPGNARAEISMART
                     ERYEKTGKPPIVTPATIHEDLRRRDFTVNAMALSLNEGSRGLLLDPFNGVADIEAKLI
                     RILHNYSFLEDPSRLIRATRFAARFHWPLEERTQARYDAAKEGKYIDHILRTAIGHEI
                     AALAHEDDPLHVVKMLEKEGWLEILHDRWTSAKVDANGIGALMKTRQQMVDLGYAPDA
                     GPAVMYFLTARLSDKDISDLQKLIPRKDLVEAWRDIGDHAKELAKRLMGKEAATPSRT
                     WNLLSAARPEMVLFLAVTAKQQSVEQKIKNFLTKWRQVQQKIPLPEMTELLITPQLPE
                     YPKIARDVFMLLLDGKLRSRTETLKFLKPFAPPPPPPPPAPRRGRAAKAAVAAPVPTA
                     TAKAGKKGKAAVPAAPAAPPPAPLKPEPKAKTPAPAKAVKSVPKAGKLVGKAAKKSSA
                     KSVEKKDRGSKTKRKK"
     gene            3656..4132
                     /locus_tag="VEJ00_14610"
     CDS             3656..4132
                     /locus_tag="VEJ00_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02445.1"
                     /translation="MPSTHKKVIVRKMDRDSVSGYVAPAQFVHEGKLEVLNTSGTVIS
                     IDLREIKGVYFVREFGDSESLARKTFTSRPRAEGLWVRLRFKDSEVLEGLMPADLAQT
                     MPEGFLISPPDQRSNTQRIFVPRTALESLTVLAVIGATRRKRRGAEDQRQVPMFGE"
     gene            4582..8181
                     /locus_tag="VEJ00_14615"
     CDS             4582..8181
                     /locus_tag="VEJ00_14615"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HXX02446.1"
                     /translation="MRAKAVWGSFCSFVTLSTLTAVVLSILFTATMLSAPAFAQDAEI
                     AGLPMPQTRITQAIDESQLTRLKGNTHPLARPQYDLGMAPATLPMERMLLVLKRSDEQ
                     EYALRTLLDNQQDKNSPNYHKWLTPEQFGNAFGPSDADMQTIVGWLQSHGFQVAAPSK
                     GRTVIEFTGSASQVQEAFHTTIHKYLVNGEQHWANSSDPSIPTALTPVVGGVYTLHNF
                     VKRAHYRRLNETFEAKFRPGLPPAFTASNGAHAVVPADLATIYNINPLYNATPTPING
                     SGISIAVVGRSDIILQDITDFRSVFAINGPTPQVIVNGPDPGDVPGDDFEATLDVSWA
                     GGIAPGAQVYFVNSAITDTTDGVILSEVYIVENNLANVMTASFGGCEARFGAEASFDA
                     GLAEQAAAQGISFFASSGDAGAEDCDDPNSEAVATGPISADLPGTTPFVTAVGGTMFN
                     ENGNNAAFWNTNDNQTNLGSAIKYIPEDVWNESCPASSCGANANIAAGGGGASAIFTK
                     PSWQAGVTGIPTDGARDIPDVALTAAGHDPYLICFEASCVPNNGQISFAAVAGTSAST
                     PSFAGIMALIDQKMSTLPAPDNSPRQGLANYVLYPLAAAENSNLSQCKATNPPANTCV
                     FNDVTVGNNEVPGEPGYPNAPYNAGVGYDEASGLGSVNVTNLANAWAGATFHPTTTTL
                     VLSPTTGITHGTTPVNVTINVKPSSGSGVPTGDVSLIAATGPNGTVSDQTGVDGFTLT
                     SGSTPANSTTSLLPGGSYKVHAHYAGDGTFAPSDSSTVTVTVQPEGSTTTFTAQTFAL
                     SGNQLSFVPLGSGTGYGNMVYFHATVASDSTNGNPTGTVTFADAAGDPAIPGNPYGLT
                     ADPGEGTGVSSAVTTQGLITLPAGPYLVKANYSSDPSFTASSSAPMAFTISQATTSST
                     VVSSANSVSPGTSVTLTANIATGTATVPSFGVAPTGTVSFKAGTTSIGTAPVTGTAGS
                     GALTNGSFTPSSATASLTTTTLPTGSDTITAVYGGDTNYAGSTSTGITVNVQPDFSLP
                     NSGLGTVTISAPGGSGTVNLSITPGTGFSSAVNFTCTAGLPAEAACNPASIAAGSTTG
                     TMTVTTMGPHTVALRNGQRQNYVAWMAMGGGLAFGCVFLAGGTRKRRWMLLMSLVVLM
                     MLVMLPACGGSGNGGGGSHQDPGTPTGTFTVTVTAAASGGSPSHTTTFSLVVQ"
     gene            8610..>9285
                     /locus_tag="VEJ00_14620"
     CDS             8610..>9285
                     /locus_tag="VEJ00_14620"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HXX02447.1"
                     /translation="MKLSAIASALGARLENGSPEAEITGLNGIERAGPGELTFVSNRK
                     YAAAARTTRASAVIVGEDFPAIAAAMLRAKDPYLSFARALELFHPPVRYAAGVHATAV
                     VDATAKIVANAHIGPYVVVGEDVVIGAGAVLLAHVVIYRGARIGDNFFAHAHAVVREN
                     CRIGNNVLLQNGVVVGADGFGFAKTAGGKWHKIPQPAPVVIGDDVEVQANSCIDRASV
                     GDTRIGR"
BASE COUNT         1780 a         2723 c         2953 g         1829 t
ORIGIN      
        1 ggcatgtaaa acggaaggcc gggaacggtg aactggcgat tggaccaatc cggatagtct
       61 ttacgagtgt aggagtagga aaaatgaaac ggctcggtga ccgcttcggg ggagctggcg
      121 ctcacctcat tgacgaggcc agcgaagcca aggccgtagg aaatctgctg cacgaggtct
      181 ttccattgcg gctgcggcac gcggcggaac gcggagcgga gcacgatttc cgcatcgcct
      241 ctggaagtgt cctcgatgtg ggcctggaat gtggcatcgt cgttgagctt gccttcaatc
      301 ttgaaagtct gcgcgctggg aaacggagga tccgccggcg tggtagtaag agtgaaggac
      361 ttctcgcccg ccataaccag cgcgggcttg tcgcgcaaag gcagcatgag aaaaccgggc
      421 ggactcactt cgggcgtcgt gtcgagccag agggccgtct tcccctgagg cagatagccg
      481 atgacgtggt cgaattgccc cggcgaggga acgtcgggat cgagcgcatg ggtggcgctg
      541 atcagtgcgg gatagatcgt gaggcccgat gcctggagca aggacgcaag cagtgtgtgc
      601 ttgtccttgc agtcaccgta gttgttcgag agcacatcgt cagcggcgtg cggctgatag
      661 cgtccgatgc cgaaagcgat gccgatataa cgatactgcg tgcttacaaa actgtagagt
      721 gcctgcagtt tagcggcatc gtctgtcgtg cctttggtta attccccagc cttggcgcgg
      781 atggcaggcg atggctcaat tcgatctttc tgaagattcc aataccagct gccgacttca
      841 tcccagctct gaaagctgct cagctgcacg tcgggagcgg gaaggcgccc gagcgccgca
      901 tcccgcttct gcttttcgct ctcgacctcg ggcttccctt ccagattcgt gtaagtccag
      961 gcgtaaaccc tggagtcgcc ctgagtattc acaacttgag ttgcctgcgg ccccttgtac
     1021 ttgaccgcgc ggctggccgg gacccgcact tccagcctct cgttgagcac gatgccatcg
     1081 tggtgaaatg aatactcgag ccagaattga ccagggatca gaggtttggt gggacgccag
     1141 tgcgcctgat actcgagcac atctcccttg ccgagtccct tgaccgcaac atgtttctcg
     1201 cgaaggtcgc tgtagaaagg ggcggcgcgg gtaatttcag catccagatc ctggatattg
     1261 tcagatggag tggtgacggt cgtgttgtcc gactttcgca cgcgcacata gttcacctcc
     1321 accgtctccg ccgctgattg atacggaagg ctgagcaggc cccattcctg cacaccggcg
     1381 tcggtttgca cgcgcacccg cgtaatctgc tcgcgagtgc ggttgccctc gttgtcgaag
     1441 gtgatgttgg tggacatttc ctctatgaca tacgcctgct gcgagtagtc gggcttggct
     1501 gcagcctggg acgcggccgc agtctgcgag tagattcgca gagccagact aagcaggccg
     1561 agggtgagtg agagacatcg actgatcatg acgtgcctgt atttatcgtt cccgccgaac
     1621 caaaaggcaa gcaggaacag caagaaccgc cgaggtcgcg ccgcgtggca gcgctgaaac
     1681 gaagctgagg cggaggcagg gactccactg cgctgagact tgcttttact tcttcctttt
     1741 cgttttgctc ccgcggtctt tcttctccac ggacttcgcc gaagacttct tggctgcttt
     1801 gccgaccagt ttgcccgcct tcggtactga tttcacggcc tttgccggcg cgggtgtctt
     1861 cgccttgggt tcaggcttca gcggtgcagg cgggggtgca gctggggcgg caggtacggc
     1921 cgcttttccc ttcttccccg ctttcgccgt cgcagtcggc acgggagcag ccactgcggc
     1981 cttggcggcg cgtccgcggc gcggcgcagg gggcggaggc gggggtggcg gggcaaaggg
     2041 tttcaggaac ttcagcgttt cggtgcgcga gcgcagcttg ccatcgagta gcagcatgaa
     2101 cacatcgcgc gcgattttcg gatactccgg cagctgtggc gtaatgagca actcggtcat
     2161 ctcgggcaac ggaatcttct gctgcacctg gcgccacttg gtcaggaaat tcttgatctt
     2221 ctgctcgacc gactgctgct tcgctgttac tgccaggaac agcaccatct ccgggcgtgc
     2281 cgccgaaagc agattccagg tgcgcgaagg cgtggcggct tcctttccca tcaggcgctt
     2341 ggccagttct ttagcgtgat ctccgatgtc gcgccaggct tcgaccaggt ccttgcgcgg
     2401 aatgagcttc tgcagatccg aaatgtcctt gtcggaaagg cgcgcggtca ggaagtacat
     2461 gaccgcgggg ccggcgtcag gcgcgtagcc gaggtcgacc atctgttggc gcgtcttcat
     2521 cagcgcgccg atgccattgg cgtcgacctt ggcgctggtc caacgatcgt gcaggatttc
     2581 gagccagcct tccttttcga gcatcttgac gacgtgcagc ggatcgtctt cgtgcgccag
     2641 cgcagcgatt tcgtggccaa tcgccgtacg cagaatgtga tcgatgtact tgccttcctt
     2701 ggcggcgtcg tagcgggcct gggtgcgctc ttcgagcggc caatggaagc gggcggcaaa
     2761 gcgcgtggcg cggatgaggc gggaaggatc ttcgaggaac gagtagttgt gcaggatgcg
     2821 gatgagcttg gcttcgatgt cggccacgcc attgaaggga tcgagcagca aaccgcgcga
     2881 gccttcattc agcgagagcg ccatggcgtt gaccgtgaag tcgcggcggc gcaggtcttc
     2941 gtgaatggtg gcgggcgtga caatgggagg cttgccggtt ttttcgtagc gctcggtgcg
     3001 cgccatgctg atttcggcgc gggcgttgcc gggcagcgag atgaagatgg tgcgggcgtc
     3061 ctcatcgacg gcggtgatgc gggcaccggc cttttcgaag tctttctgca gcttgagagg
     3121 attgccctgg acggtgaagt cgagatcgcg aatgctgaag ccgctgatga tgtcgcgaat
     3181 ggcgccgccg gtcaggtaaa gattcatgcc cgcggcgcgg gctacatcct gcactaggcc
     3241 gacgcccttt aactggtccg gcgtcaaccg gagctccatc gtgtagatgt aatcagccat
     3301 tcgcgttcaa acaggccttg ctggcgctgc acacgcttcg ccccggctgg gctctaaggg
     3361 gcagcgctct ctccacaaga tttccacagg cgcttgcgac cgacggcacg aaaggctccc
     3421 acgccaggtg caagtgacta gatataaagc agttagcctt ggagaaatct ttgcggcaag
     3481 ctaaagaaaa taacacaacg tttacatttt ggctactggc aaagccgatt cggggagttt
     3541 cttgaagaaa agtgcgaaaa tcgagaagca gggcccaggg atcaggggtc agggaccagt
     3601 aagatcacgc gctcttgctg acccctgacc cctgatccct ggtacctgcc ttctgatgcc
     3661 ctcaacgcac aaaaaagtga tcgtccgcaa gatggaccgc gactcggttt cgggctacgt
     3721 cgcgccggcg cagtttgtgc atgaaggcaa gctggaggtg ctgaatacct caggcacggt
     3781 catttcgatt gatctgcgag agatcaaggg cgtttacttc gtgcgcgaat tcggcgactc
     3841 ggaatcgctg gcgcgcaaga cgttcacctc ccggccgcgc gccgaggggt tgtgggtgcg
     3901 gctgcgcttc aaggacagcg aagtgctgga aggcttgatg cccgccgacc tcgctcaaac
     3961 catgccggaa ggattcctga tcagccctcc cgaccagcgg tcgaacacgc agcggatctt
     4021 cgtgccgcgg acggcactgg aatcgctgac cgtactggcc gtgatcggag cgacgcgccg
     4081 gaagcgccgc ggggctgagg atcagcggca agtgccgatg ttcggcgagt agcggttgtc
     4141 gccgacgcga acaccttgcg cttttacacg cacctggcgc tgccagctgg acagtcaagt
     4201 catcgtccca tctaagtgct tgttcgcccg tatcttgcag ctcatcgcta ccagcgcgac
     4261 gcggctgcgg cagggcattc cgcgagtcgc cgtggcacaa atcgtgggcc tggcaggcct
     4321 ctggcgagcg aaaagcagca tccaagttcg cagtcaggac gccgcctaag ctcagatttc
     4381 gtgtaagaat cagcggcgga catgtactaa ctctggctga acttgtccat caaagtcgtt
     4441 gaaaagcacc gcgatattga ggtagtgtga ctttcaccgg ggcattggct cccggaacgg
     4501 gacgaatcga gccctcccca tcccctgatc tcattcgatt tccgttggcc gtctgaactt
     4561 ccgtgtcgag gtatgtctgc tatgcgagca aaggccgtct ggggctcttt ctgttccttc
     4621 gtcacccttt caacccttac cgcagttgtg ttgtcgattt tgttcacggc gaccatgctg
     4681 agcgcgccgg cgttcgcgca agacgcggag attgctggcc tgccgatgcc gcagactcgg
     4741 atcacgcagg cgatcgacga gtcgcagctg acgcggctca aggggaatac gcatccgctg
     4801 gcgcgtccgc agtatgacct gggcatggct ccggccacgc tgcccatgga gcgcatgctg
     4861 ctcgtgctca agcgcagcga cgagcaggag tacgcgctgc gcacgctgct cgacaaccag
     4921 caggacaaga actcgcccaa ttatcacaag tggctcacgc ccgagcagtt cggcaacgcg
     4981 tttggcccga gcgacgccga catgcagacg atcgtcggct ggctgcagtc gcacggattc
     5041 caggtcgccg cgccctcgaa gggacgcacg gtgatcgagt tcaccggctc cgccagccag
     5101 gtgcaggaag cgtttcacac caccatccac aagtatctgg tgaacggcga gcagcactgg
     5161 gcgaactcaa gcgaccccag catcccgacg gcgctgacgc cggtggtggg aggggtgtac
     5221 acgttgcaca acttcgtgaa gcgagcgcac taccgcaggc taaatgaaac cttcgaagca
     5281 aagttccgtc ccgggctccc gccggcattc accgccagca atggggccca tgcggtggtg
     5341 cctgcggacc tcgcgacgat ttacaacatc aacccgctct acaacgcgac tcccactcca
     5401 atcaacggca gcgggatctc catcgccgtt gttggacggt ctgacattat cttgcaggac
     5461 atcaccgatt tccgaagcgt attcgcaatc aacggtccga ctccgcaggt catcgtgaat
     5521 ggccctgatc ccggagatgt tccgggggac gatttcgagg ccacgctgga tgtgtcctgg
     5581 gcaggtggaa tcgctcccgg tgcgcaagtc tattttgtga attcggcgat caccgacacc
     5641 acggacggcg tgatcctgtc cgaggtttat atcgtcgaaa acaatcttgc gaatgttatg
     5701 acagcgagtt tcggcggctg cgaagcccgc ttcggcgcag aggccagctt cgacgcgggc
     5761 ctggcggaac aggcggctgc acaaggcatc agcttttttg cctccagtgg agatgccggc
     5821 gctgaggact gcgatgatcc caacagcgag gccgtggcca cggggccaat ctctgcggat
     5881 ctgccgggca cgactccttt cgtcaccgcc gtgggcggaa cgatgttcaa cgagaacgga
     5941 aacaacgccg cgttctggaa caccaacgac aatcagacca acctcggttc tgcaatcaag
     6001 tacattcccg aggatgtctg gaacgaaagc tgccctgctt cgagctgcgg tgcaaatgcg
     6061 aacatcgcag ccggaggtgg gggagccagc gcaatattca ctaagccttc ctggcaggcg
     6121 ggagttaccg gaattcccac tgacggcgcg cgagacattc ccgacgtcgc tctgacggcc
     6181 gctggtcatg acccctatct gatttgcttc gaggcatcgt gcgttccgaa caacggtcaa
     6241 atcagcttcg cggcggtcgc agggacgtcg gcctcaaccc cttcgtttgc gggcattatg
     6301 gcgctgatcg accagaagat gagcacgcta ccggcgcccg acaacagccc tcgtcagggc
     6361 ttggcgaact acgttctgta tccccttgcg gcggcggaaa actcaaacct gtcccaatgc
     6421 aaagccacga acccgccggc gaacacctgt gtttttaacg acgtgactgt ggggaataac
     6481 gaggtcccgg gagaaccggg ctacccgaac gctccgtaca atgctggggt cggctacgac
     6541 gaggcctccg gtctgggatc ggtcaatgtc acgaatctcg cgaacgcctg ggcgggagca
     6601 acgttccatc cgaccacaac aactcttgtc ctcagcccta cgactggcat cacgcacggc
     6661 accacccccg tgaacgtgac tatcaatgtc aagcccagca gcggaagcgg ggtacccacc
     6721 ggcgacgtct cactcatcgc tgcgactgga ccgaatggca ccgtttcgga ccaaaccgga
     6781 gtagacggat tcacgctgac gagcgggtct acgccggcaa actcaaccac cagtttgctt
     6841 cccggcggat catataaagt tcacgcccac tacgcgggag atggcacgtt tgccccgagt
     6901 gactcgagta ctgttacggt gaccgttcag ccggaaggca gtacaaccac ctttaccgca
     6961 cagacctttg ctttgagcgg caatcagctc agcttcgtcc cgctcggcag cggcacgggc
     7021 tacggcaaca tggtctactt ccacgcaacg gtagctagcg attcgacgaa cggtaacccg
     7081 actggcaccg tcacgtttgc cgacgccgcc ggcgatccgg cgattcccgg caatccgtat
     7141 gggctgacag cggacccggg tgaaggcacg ggtgtgagtt ctgcggtgac tacccagggc
     7201 ttgatcactc ttccggcagg gccgtatttg gtgaaggcga actacagttc cgacccgagc
     7261 ttcacagcca gttcttcggc gccgatggcg tttacgatct cgcaagccac cactagcagc
     7321 actgtggttt cgagcgctaa ttctgtctcg cctgggacca gtgtgacgtt gacggcgaac
     7381 atcgcgacag gcactgccac agtgccaagc tttggtgtgg caccgacggg tacagtctca
     7441 ttcaaggccg gcaccacatc aataggaact gcaccagtga cgggaacggc tggctccggc
     7501 gcgcttacga acgggtcgtt cacgccctct tcggcaacag caagcttgac cactaccacc
     7561 ttgcccacgg gatcggacac catcaccgcc gtctacggcg gggataccaa ctacgccggc
     7621 tcgacgtcca ctggaatcac cgtgaatgtg cagccggact tcagtctgcc gaacagcgga
     7681 ttggggacgg tcacgatcag cgctcccggc ggatcgggaa cggtaaatct ctcgatcacg
     7741 cccggcacag gcttcagctc cgcggtcaac tttacctgta cggcggggct gccggcggaa
     7801 gcagcctgca atcctgcttc gattgcggcc ggatcgacca caggcacaat gacggtgacg
     7861 accatgggtc cgcacacggt cgcgctgcgc aatggtcagc ggcagaacta cgtggcctgg
     7921 atggcgatgg gcggcgggct ggcgtttggc tgcgtgttcc ttgcgggagg aacgcggaaa
     7981 cggcgctgga tgctgctcat gtcgctggtc gtcctgatga tgctcgtcat gctgccggcg
     8041 tgcggcggca gtgggaacgg cggtggcggg tcgcaccagg atccgggcac gcctacggga
     8101 acatttaccg tgactgttac cgcagcggcc agcgggggat caccgtcgca cacgacgacg
     8161 ttcagcctgg tcgtgcagta agcagcgagg ctcatcttaa caacgaaggc caacgctcgg
     8221 gcgttggcct ttgttctgcc gtcatgaccg ggcttagcgg gcgatcgaca ggccgcctac
     8281 aggggcattc gtcgaattcg aacgtgtcct caagcgagtg aggggagagg gcacgagttc
     8341 cactcgtgcc acaagcgtca aggggagcgc ggcggcttta gccgctgagg tcggcttcgg
     8401 cctggcgcgg gtgaacgagg caaagacgac cctcaggcgc tgaagcgcac gcatatttcg
     8461 gcctttacgg cacgactgaa gtcgtgccct tcgcaaaccg gggcaggcaa ggttcccggc
     8521 ggttccgctt tgacggcttc cttgtggtgt acgatctatt cgtcgtttgc caatgtaaag
     8581 cgatccactg cgtggcaaac cattcatcca tgaaactttc tgcgattgct tctgctcttg
     8641 gcgcgcggct agaaaacggc tctccggagg cggaaatcac cgggctcaac gggatcgagc
     8701 gggcggggcc gggggagctg acgtttgtgt cgaatcggaa atatgcggcg gcggcgcgga
     8761 cgacgcgggc ttcggccgtc atcgtgggcg aggacttccc tgcgattgcg gcggcgatgc
     8821 tgcgggcgaa ggatccgtac ctgagctttg cgcgggcgct cgagctgttt catccgccgg
     8881 tgcggtatgc ggcgggcgtg catgcgacgg cggtggtcga cgctacggcg aagattgtag
     8941 cgaatgccca catcgggccg tatgtggtgg tcggcgagga cgttgtgatc ggggccggcg
     9001 cggtgctgct ggcgcatgta gtgatctacc ggggagcacg gattggcgac aatttcttcg
     9061 cgcacgcgca tgcggtggtg cgggagaact gccgcatcgg caacaacgtg ctgctgcaga
     9121 atggcgtggt ggttggggcc gacggattcg ggtttgccaa gacggccggc gggaagtggc
     9181 acaagattcc gcagccggcg ccggtggtga ttggagacga cgtcgaggtg caggcgaact
     9241 cgtgcatcga ccgggccagc gtgggagata cgcggattgg acgcg
//
LOCUS       DASPUL010000634         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1897471, whole genome shotgun sequence.
ACCESSION   DASPUL010000634 DASPUL010000000
VERSION     DASPUL010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1897471"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VEJ00_14625"
     CDS             <1..511
                     /locus_tag="VEJ00_14625"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HXX02448.1"
                     /translation="YEAPFYTLGPLVTDIAPGYDHITSAIGAAMIGWYGAAMLCYVTP
                     KEHLGLPNEKDVKDGIIAYKISAHAADIARGRPGARDRDDALSYARYKFDWEKQFALS
                     LDPETARSMHDETLPDDYYKEAAFCSMCGPKFCSMNYSSKVDEYNKQVHGIEKKDYSE
                     LVEKLVTLK"
     gene            complement(785..1030)
                     /locus_tag="VEJ00_14630"
     CDS             complement(785..1030)
                     /locus_tag="VEJ00_14630"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXX02449.1"
                     /translation="MKRIVWTDPARSDIRAISNPVAMQILSALHRLAESGVGDIKTLQ
                     GREELRLRVGDYRLFFVCPDSNTLEIRRVLHRGEAYR"
     gene            complement(1027..1305)
                     /locus_tag="VEJ00_14635"
     CDS             complement(1027..1305)
                     /locus_tag="VEJ00_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02450.1"
                     /translation="MAADKQHAHELIERLAPSQVSAVVDMLEAMLDPVARAMAGAPVD
                     DEELTAADEKALDEASEWLKHNPGIPHEQVLAELGLTAKEIKRSQRRK"
     gene            complement(1430..2077)
                     /locus_tag="VEJ00_14640"
     CDS             complement(1430..2077)
                     /locus_tag="VEJ00_14640"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HXX02451.1"
                     /translation="MHSAHALGQFLRTLPASALATFLALFPIVDPPGGVPIFFTMTSS
                     WTPRERSHTAFKTGIWVFGILVAFLFFGRFVLHFFGISLPVLKIAGGLIVANTAWGMV
                     TSHSRITPEETYEAQDKEDISLTPLAMPLMSGPGAIGVVMALAAHVDNMAAYIGMVIG
                     IAAVALSVALFFCLGGPLVRRLGPASVGAINKIFGFLILAIAVQLVWNGVADFNS"
     gene            complement(2081..>3082)
                     /locus_tag="VEJ00_14645"
     CDS             complement(2081..>3082)
                     /locus_tag="VEJ00_14645"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HXX02452.1"
                     /translation="GWKPKRTMVFCSWDGEEEGLIGSTEWVEQHESELAHSPAYFNVD
                     VAVSGPKFGASAVPSLKQFLRDVTKVVPSPKGGTVYEAWEKANLPSAPSTQSPTEAIG
                     DSRRTPAAQVKNDVPVGDLGSGSDYTGFLQHLGVPSTDVSSTGSYGVYHSTFDDFAWF
                     KKFGDPDFAYEQQMARVFGLEAVRMADADVLPYDYEEYGKEIDAYLETARKRAEAKFG
                     DRALDFGPVTAAARRFETAGAKILAKQQNPPRDPARLNQALLNAERALIVPEGLPHRP
                     WFRHAIYAPGEYTGYAAVVIPGVNEALDKGDSQRAREQLAVLTAALDRAAKTLEAYK"
BASE COUNT          640 a          887 c          976 g          579 t
ORIGIN      
        1 ctacgaggct ccgttctaca cgctcggtcc gctggtcaca gacatcgctc ccggatacga
       61 ccacatcacg tcagcgatcg gagcggcgat gatcgggtgg tacggcgcgg ccatgctgtg
      121 ctacgtcaca ccgaaagagc acctcggcct gcctaacgaa aaagacgtaa aggacgggat
      181 catcgcctac aagatttcag cgcatgccgc cgatatcgcc cgcggacgcc caggcgcccg
      241 cgaccgcgac gacgctttga gttacgcgcg gtataaattt gactgggaga aacagtttgc
      301 gctgtcgctc gatccggaaa ccgctcgctc gatgcacgat gagaccctgc ctgacgacta
      361 ctacaaagag gcggcgttct gctccatgtg cgggccgaaa ttctgctcga tgaattattc
      421 gtcgaaggtc gacgagtaca acaagcaggt gcacggcatc gagaagaagg attattcgga
      481 gctggtggaa aagctggtga cgctgaagta ggcatccagc gcgggttcac tcacaaccgg
      541 gggcgcagcg aagatcgcga agagcacaag acattccaag attactgggg attttgccga
      601 agcagtcgta ctgtattggc tctcgaagag tggcatctct gccagctttc gccccaaaag
      661 aagcgtgcgg cggtggaaga tgactgaaag ctatctgaaa cgatattcgg acgaccctca
      721 gatcatgtcg ttcgaattca agactacaac gactcggtgg tgggaaggtt gatgggcatc
      781 taggctatcg gtaggcctcg ccacgatgta gcacgcgccg aatctcgagc gtattggaat
      841 ccgggcagac gaaaaacagg cggtagtcgc cgacgcgcag gcgtagttcc tcacgtccct
      901 gaagggtctt tatgtcgcct acgcctgact ccgccaagcg gtgcagggcg gagagaatct
      961 gcatagcgac ggggttactg atggcgcgga tgtcactcct ggcgggatcg gtccaaacga
     1021 tccgtttcac ttccgtctct gagatcgttt gatttctttt gctgtaagac cgagttccgc
     1081 aagaacctgc tcatgcggga tgcctgggtt gtgcttcagc cactcagaag cttcgtcgag
     1141 tgccttctcg tcggctgcgg tcagctcttc gtcgtcaact ggtgcaccag ccatcgcgcg
     1201 cgcgacggga tcgagcatgg cttcaagcat atcgaccacg gccgagacct ggctgggggc
     1261 caggcgctcg atcagttcgt gggcgtgttg cttgtcggcg gccattagcg tctccacaaa
     1321 cattctaact gagagcacca aacggttgag atcccgccct ttgacaatcg gggagggtgc
     1381 gcagccggcc gtgatagccc tgtgaacttc cgagacaagg gctccacact cagctgttga
     1441 agtcggcgac gccgttccac accagctgaa ccgcgatggc gagaatgagg aagccaaaga
     1501 ttttgttgat ggcgccgacg gaggcgggac ccaaccggcg aaccagcggg cctcccaggc
     1561 agaaaaacag ggccacgctg agcgcgacgg cggcgatgcc gattaccatg ccgatgtacg
     1621 ccgccatgtt atcgacgtgt gcggccaaag ccatgactac gccgatcgcg cccggaccag
     1681 acatcagcgg catcgcaagc ggagtgagcg agatgtcttc cttgtcctgg gcctcgtagg
     1741 tttcttcggg cgtgatgcgc gagtgcgacg tgaccatgcc ccaggcggta ttggccacga
     1801 tcaggccgcc ggcgattttg agcacaggca acgagattcc gaagaagtgc agcacgaagc
     1861 ggccgaagaa gaggaacgcc accagaatcc cgaaaaccca gatgccggtc ttgaacgcgg
     1921 tgtgcgagcg ctcgcgcgga gtccatgagg aagtcatggt gaagaagatg gggacgccgc
     1981 ccggagggtc gacgatcggg aacagcgcga gaaaggtcgc cagcgccgaa gctggcagcg
     2041 tgcgcagaaa ctggccgagg gcgtgggcgg agtgcatggg ctatttgtac gcttccaatg
     2101 tcttcgcggc gcgatcgagc gcggcggtga gcacggctaa ctgctcgcgg gctcgctggc
     2161 tgtcgccttt atcgagcgcc tcgttaacac ccggaatcac aacggcggcg taaccggtat
     2221 attccccggg agcgtagata gcgtggcgaa accagggacg atgcggtaat ccctcaggca
     2281 cgatcagggc gcgctcggcg ttgaggagcg cctgattgag gcgggcggga tcgcgcggag
     2341 gattctgctg ctttgccaga atcttcgcgc ctgccgtttc aaagcggcgt gcagccgcgg
     2401 ttactggacc gaaatcgagg gcacgatcgc cgaacttggc ttcggcccgc ttccgcgcgg
     2461 tctcgagata cgcgtcgatt tcttttccat actcctcgta gtcgtatggc aacacatcgg
     2521 cgtcggccat gcgcacagct tccagtccga agacccgagc catttgctgc tcgtaggcga
     2581 agtcaggatc gccgaatttc ttgaaccagg cgaaatcgtc gaaggtcgag tgatacacac
     2641 cgtaggaccc ggtcgaactc acgtcggtgg agggcactcc caggtgctgc agaaagccgg
     2701 tgtagtcgga accgctgccg agatcgccca cggggacgtc atttttgacc tgagcagctg
     2761 gtgtgcgccg gctgtcgccg atcgcttcgg tcggggactg cgtggaaggc gccgagggca
     2821 gattcgcctt ctcccaggct tcgtaaacgg tgccgccttt agggctgggc acaactttcg
     2881 tgacgtcgcg caggaattgc ttcaggctcg gcaccgccga ggctccgaac ttcggtccgg
     2941 agacggcgac gtccacgttg aaatacgcag gggagtgggc gagctcagat tcgtgctgct
     3001 cgacccactc ggtcgacccg atcaggcctt cttcctcgcc atcccagctg cagaagacca
     3061 tggtgcgctt cggcttccag cc
//
LOCUS       DASPUL010000635         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1076965, whole genome shotgun sequence.
ACCESSION   DASPUL010000635 DASPUL010000000
VERSION     DASPUL010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076965"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1182
                     /locus_tag="VEJ00_14650"
     CDS             274..1182
                     /locus_tag="VEJ00_14650"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HXX02453.1"
                     /translation="MIRTRRSCAVLFTLMSFLALGRARLAHAQTASLPPKLSVHWEEL
                     TAADFREGIRRSQGTCLLPFGILEKHGPHLPLGTDLLDVRYAALHAAEQEYAVVFPEY
                     YFGQIAEAKHEPGTIAYSRSLQMALLQETTDEMARNGCKKVLIVNGHGGNEHLLPYFA
                     QTQLDKPHDYVVYVFDERTPQAGGPAKKTKIDMHAGESETSKMMISRPDTVHLDRATN
                     ESGADQARQKLPDGVYTGIWWYARFPNHYSGDGSAATKELGQYQMSWWIDSVVKAIKA
                     IKADDVSLKLQTEFYEKTTHPLETQP"
     gene            complement(1301..2257)
                     /locus_tag="VEJ00_14655"
     CDS             complement(1301..2257)
                     /locus_tag="VEJ00_14655"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HXX02454.1"
                     /translation="MKKFEDLTEREVLALAITLEEEDERVYSDFAEGLRADYPATASM
                     FEGMREEESGHRRRLLELFRSKFGEHIPLIRRQDVRGFVERPALWLVRPLRIEAVRKE
                     ASAMEVETRRFYEKAAARTQDASIRQLLDDLANEERTHETRAQELDKQKLPDNVKEDE
                     EKAQRRLFVLQIVQPGLAGLMDGSVSTLAPVFAAALATQKSWDAFLVGLAASAGAGIS
                     MGFAEALSDDGSLTGRGHPWVRGIVCGAMTAVGGIGHTLPFLIHDFRLALWAAAAVVL
                     AELGVITWIRRRYMDTPWVTAALQVGLGGVLVFITGVLIGSS"
     gene            2429..>2665
                     /locus_tag="VEJ00_14660"
     CDS             2429..>2665
                     /locus_tag="VEJ00_14660"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HXX02455.1"
                     /translation="MSGEGKGKRSTRSQGKPRRERKNSPELVIITGMSGSGKASVLKA
                     FEDLGYYCVDNLPVELIPQFAELSMQSSESRRTAL"
BASE COUNT          553 a          764 c          856 g          492 t
ORIGIN      
        1 gggaagcttg aaccaggtcc tgcccccagg aaagatccgt cggcagaatg acggcgagca
       61 acagaatcgc tacagtttgc caggggcgtt tcaagtccgc tcctcttgca ggatatcgat
      121 ccatcatcaa ggtactgccg gcgctttcga ttagtaagtg aaacacaagg ttcgccgaga
      181 gccggcgagt gggcgcctgc aaggacgctg gtattgatac gttttaatac ggggaaattg
      241 cgtgctagaa tgcgcggcgg gagattcctt accatgatcc gtacccgccg tagttgtgcc
      301 gtacttttca ccctgatgtc gttcctcgcc ttgggacgag cacggctcgc gcacgcgcag
      361 actgcttcct tgccgcccaa actttcggtg cattgggaag aactgaccgc agctgacttc
      421 cgcgaaggca ttcgccgatc gcagggaacc tgcctgctgc ctttcggcat tctggagaag
      481 cacgggccac accttcccct gggtactgat ctgctcgacg tgcgctacgc cgcgctgcac
      541 gcagccgagc aggagtatgc tgtggttttt cccgagtatt actttgggca aattgccgag
      601 gcgaagcacg agccgggcac gatcgcttac agccgcagcc tgcagatggc tcttctgcag
      661 gagaccaccg acgagatggc ccgcaacggc tgcaagaaag tgctgatcgt caacgggcac
      721 ggaggcaacg agcacctgct gccctacttt gcgcagacac agctcgacaa gccgcacgac
      781 tatgtggttt acgtattcga cgaacggacg ccgcaagctg gcggtcctgc aaagaaaacc
      841 aagatcgaca tgcacgcggg cgagagcgaa acgtcgaaga tgatgatttc gcgcccggac
      901 acggtgcatc tcgatcgcgc cacgaatgag tcgggcgcag accaggcgcg acagaagctg
      961 cccgatggcg tctacacggg aatctggtgg tacgcgcggt ttccgaatca ctattcaggc
     1021 gacggatcag cggcgacgaa ggaactcggc cagtatcaga tgagctggtg gatcgattcg
     1081 gtggtgaagg cgatcaaagc gattaaagcc gacgacgtca gcctgaagct gcagactgag
     1141 ttctacgaga agacgacgca tccgctggag acgcagcctt gaagcgactg cagattgatg
     1201 atttcaagat tgcagattga gagggcatca gcgactcgac gcgaacggaa accattccag
     1261 aatctgcaat ctgaaatctg caatctgaga tctaacgtct ctacgagctt ccaatcagca
     1321 cgccggtgat aaacaccagc acgccgccga ggccgacctg cagggcggcg gtgacccagg
     1381 gcgtatccat gtagcggcgg cggatccagg tgatcacgcc gagttccgcc agtacgaccg
     1441 cggcagcggc ccacagcgcc aggcggaaat cgtgaattag aaatggcagc gtgtggccga
     1501 tgccgccgac ggcggtcatg gcgccgcaga cgatgccgcg tacccaaggg tgtccgcggc
     1561 cggtgaggct gccatcgtcg gagagagctt cggcgaagcc catgctgatg cccgcgccgg
     1621 ccgaggcggc gagcccgacg aggaaggcat cccagctttt ctgcgtggcc agcgccgcgg
     1681 caaaaaccgg ggcgagagtt gagaccgagc catccatcag gccggccaga cccggctgca
     1741 cgatctgcag gacgaacagg cgccgctgag ctttttcttc gtcctccttc acgttgtcgg
     1801 gaagtttttg cttgtcgagt tcctgggcgc gggtttcgtg cgtgcgttct tcgttggcga
     1861 gatcgtcgag gagctggcgg atgctggcgt cctgggtgcg ggctgctgcc ttctcgtaaa
     1921 aacgcctcgt ctcaacctcc atggcggacg cctctttgcg cacagcttca atgcgcagcg
     1981 ggcgcaccag ccacagcgcg gggcgctcga cgaagccgcg cacatcctgg cggcggatga
     2041 gcgggatgtg ttcgccgaac tttgatcgaa agagctcaag caagcggcgg cgatggccgg
     2101 attcttcttc gcgcatgcct tcgaacatgg aggcagtggc ggggtagtct gcacgcaggc
     2161 cttcggcaaa gtcggaatag acgcgttcgt cttcctcctc gagggtgatg gccagggcga
     2221 gcacttcccg ttcggtcagg tcttcgaact ttttcatgac gccatagatt acccgtcagc
     2281 gcggggcgga gacaaacggg atctgcgatt ggcgtttccg gatgacgcta gggctcgtgg
     2341 aatctcgaca ggcaatgccg ggaaaaagcg cgggtggcgc ttcgatgcta ctatccagcg
     2401 tatgaaggcg cggtccagga aggcacgctt gtctggcgaa gggaagggga agcgctcgac
     2461 gcgttcacag ggcaagccgc gccgcgagcg gaagaattcg ccggaactgg tcatcatcac
     2521 ggggatgagc ggctcgggga aggcctcagt gctcaaggcc ttcgaagacc tgggctacta
     2581 ctgtgtcgac aatttgccgg tcgagctgat tccgcagttt gccgagctgt cgatgcagtc
     2641 gagcgagagc cgccgtaccg cgctg
//
LOCUS       DASPUL010000636         8622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1219809, whole genome shotgun sequence.
ACCESSION   DASPUL010000636 DASPUL010000000
VERSION     DASPUL010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8622)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8622
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219809"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VEJ00_14665"
     CDS             <1..285
                     /locus_tag="VEJ00_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02456.1"
                     /translation="LTGVVSNNGVIKIVSSTVNNSPCEPASGVYPTPSVRAWASRIQN
                     KVGSAYPVTETEFSDATLSAQELSFNLEEQCYFAERLGSGHGICSCGYGD"
     gene            complement(465..1373)
                     /locus_tag="VEJ00_14670"
     CDS             complement(465..1373)
                     /locus_tag="VEJ00_14670"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXX02457.1"
                     /translation="MKKLRFVVSLHGSGNDFQVAQAQCAESAARKLGIEVQIVFAEDD
                     AVNQSTQLLKAIQGPSESRPHAILLEPVGATALPQVAKAASTAGIGWAVLNRRPEYLA
                     ELRQQANAPVFAVSADHEEIGRIQGKQFAALLPRGGTVLYIEGPTHSSSAQKRTVGML
                     ETKPSNIQISTLKANWTGEGAARAVLAWLRLSTSQATRIDLVGAQDDAMAMGARKAFQ
                     GLANDNERAKWLTLPFTGCDGQPETGQVWVKEKLLAATIHIPPLTGIAIEILSEAMEK
                     GTQPPELTLTRPVSIPALDTLSAREP"
     gene            1615..2901
                     /locus_tag="VEJ00_14675"
     CDS             1615..2901
                     /locus_tag="VEJ00_14675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX02458.1"
                     /translation="MPPSSILGSVDVRAAHTRRVVGLRLLPFLFVLYIVNYIDRTNLA
                     YAAVGMSRTLGFGDRIFGLGAGIFFISYLALQIPGARMVARFGARRVISSCMIAWGSL
                     TVLTALVHTPIQLYLARFVLGAAEASFFPGVIVYLSYWFINEDRAKATSNFMSAIPLS
                     SMLGSPLAGWILGRTWLGVDGWRWLFVAEGLPAIGLGVVAYFFLTDRPGDATWLHPGG
                     RDWLLRTLEEESPARTAKAGFRDALQSPLICFLAAMTFLAYVAQYTFVFWFPLLFKRL
                     SGMTDLHVGLWGALPFLGAFLLMQINGWHSDKMAERRWHAAMPLFLSGAGFLLLTTRP
                     QAFPVLLAFFMMASTATVYLPTFWAIPPEILSPTIVATAVGLINSVGSIAGFAAPYAF
                     GYLQAVTGSLSAGLSVTAVCAFAAGFLILQIPKGQE"
     gene            3147..3398
                     /locus_tag="VEJ00_14680"
     CDS             3147..3398
                     /locus_tag="VEJ00_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02459.1"
                     /translation="MANDDVVKCPLCGGFTHIENPDLLAALRTPALREQVERFIAELL
                     KSPVEDLASVGATQAGRDFQKDVHNWNPCVPMWRRSPKE"
     gene            3487..4824
                     /locus_tag="VEJ00_14685"
     CDS             3487..4824
                     /locus_tag="VEJ00_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HXX02460.1"
                     /translation="MKARRILILGAAGRDFHNFNVVFRGNAEYNIVGFTATQIPGIAN
                     RRYPTELAGPLYPAGIPIFEEHDLERLVEEHEVDAVVFSYSDISHQNLMHLACRVAAT
                     RADFWLLSSERTQLKASIPVISICAVRTGCGKSPLGRLVTSELRREGWKPVVIRHPMP
                     YGDLAAQAVQRFATLEDLDTQQCTIEEREEYEPHLREGTVVYAGVDYGRILRQAETEA
                     DIIVWDGGNNDTPFYASDLEIVVCDPHRPGHELAYYPGEVNLRRADVVVINKVDTAAP
                     QDVETVRRNIRISNPNAKVVETACRVAVSAPELVKGRTVLVVEDGPTLTHGEMPYGAG
                     VVAARQCGAAELVDPRPFAVGSIRWTYEHYPHLTSLLPAMGYSAIQRHELEETINRVP
                     CDLVLVATPIDLGHLIKLNKPSLRVTYEVEDLTHPGLGEILARFTRERSGVGV"
     gene            4963..5847
                     /locus_tag="VEJ00_14690"
     CDS             4963..5847
                     /locus_tag="VEJ00_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXX02461.1"
                     /translation="MSTTPQVSRIALKNILFPTDFSQASNGALPFALSLARVYDSTVV
                     VAHALPPEPHLQVVLDRVPATDERPWHEAQDKLVQFTHNRAFGEIPCKIALDRGDLAD
                     VIPAMIREYAADLVVLGTHGRRGVSKIVLGSGAEKIYRSASCPVLTVGPKAHESAEPW
                     TLHRILCPVDLAEDPEPALHYALSLAEEHQAEFIVMQSIPLVPWQRRPAAEEHARRDL
                     ERLIPESAKDWCVPQCIVRWEYPAEAILHAAAEREVDLIVMGVHKARAATWSAHLPWP
                     VASEVVSRARCPVLTVRV"
     gene            5915..8227
                     /locus_tag="VEJ00_14695"
     CDS             5915..8227
                     /locus_tag="VEJ00_14695"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HXX02462.1"
                     /translation="MLVSKQQALDYHFGARPGKIEITPTKPCRTQRDLSMAYTPGVAE
                     PCLEIEKNPHDAFKYTGRGNLVAVLSNGTAVLGLGDIGALAGKPVMEGKAVLFKRFAD
                     VDVFDIEVNSHNPDEIIKVCQLLEPTFGGINLEDIKAPDCFYIEETLRKTMKIPVFHD
                     DQHGTAIISGAALLNALEVVGKDIRKVKVVFNGAGAAGIACAEHYVRLGVKRENIILC
                     DTKGVVHKGRKEGMNKYKEAFAIETDMRTLGEAMIGADVFVGLSVKGAINQDMVRTMA
                     HDPIVFAMANPDPEITYEDAKAARPDVIIATGRSDYPNQVNNVLGFPFIFRGALDVRA
                     TAINEEMKLAATRALANLTKEDVPDSVCRAYGVQRLQFGREYLIPKPFDPRVLMWEAA
                     AVAEAAMRTGVAQEPVDLENYRAQLERRLGKAYEVSRTMIHKAQINPKHVVFPEGDNE
                     KILRAAHSLVDEKIAHPILLGDAEKIRARAHELGLDLEGMQIVDPATSDHRESYALEL
                     FNLRQRRGVTLEEAHTLIGDRNVFGSMMVRMGDADALVSGVTQHFSETIRPALQIVRM
                     REGLHRVSGCYAMITRKGEVFFLADTSVNIEPTAEDLVEIALCTAHMARRFDMSPRVA
                     MLSFSSFGSVDHPICTKVRKAVELLHQADPTLIVDGEIAADDAVSPEVIEQEYPFSAL
                     KGGANVLIFPDLASANVATKLLAKIGGGEVIGPILMGMSRPVHVLQRTATVEEIVSVA
                     AIAVVDAQEHDIYAHPHVHVSVEPELVGED"
     gene            8379..>8622
                     /locus_tag="VEJ00_14700"
     CDS             8379..>8622
                     /locus_tag="VEJ00_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02463.1"
                     /translation="MSTLWKSRYAQRTFNIKSSAIRELLKVTQRPEIISFGGGLPAPD
                     VFPVKRFEEACHKVLTEHGAQALQYGPTEGYPPLREM"
BASE COUNT         1585 a         2824 c         2506 g         1707 t
ORIGIN      
        1 ctgaccggcg tcgtttccaa caacggcgtc atcaagattg tgtcctcgac cgtcaacaac
       61 tccccctgcg agccagcatc gggtgtgtat cccacgcctt cagttcgcgc ctgggcgagc
      121 cgcattcaga acaaggtcgg cagcgcgtat ccagtcactg aaaccgagtt ctcggatgcc
      181 actctgagcg cccaggaact cagcttcaac ctggaagagc agtgctactt cgctgagcgt
      241 ctgggcagcg gacacggcat ctgcagctgc ggttatggtg actagaacat aacgacctcc
      301 tcgcttctgc aggcggcggc ctctcaaaag gccgccgtct ttttgctcac ctgtagtgct
      361 tcccgggaga ctcagtcggc ccttcgctca taactcgagt tgctatcgtg gagggtaccc
      421 attggtgaag cgaaggtggc gacatcgaga ctgtcgccac caacttacgg ttctcgcgca
      481 gacaaggtat ccagcgccgg aatggagacc ggtctggtca aagtgagttc cggcggttgg
      541 gtgcctttct ccatcgcttc tgacaggatt tcaatcgcta ttccggtaag cggcgggatg
      601 tggatcgtag ctgccagcag tttttctttc acccagacct ggcccgtttc aggctggcca
      661 tcgcacccgg tgaaaggcag cgttagccac ttcgcccgct cgttgtcgtt ggcaagcccc
      721 tgaaacgctt tgcgcgcgcc catcgccatt gcatcatcct gtgcccctac caggtcaatg
      781 cgggtcgctt gggaagtgga cagtctcagc cacgcgagta ctgcccgcgc ggctccttcc
      841 cccgtccagt tcgctttcag cgtagaaatc tggatgttcg acggtttcgt ctccagcatt
      901 cccaccgtgc gcttctgggc cgaagaactg tgggtaggcc cctcgatata caagaccgtg
      961 ccgccccggg gcagcaaggc cgcgaactgt ttgccctgga tacggccgat ttcctcatga
     1021 tcggcactga cggcaaaaac aggcgcgttg gcctgctggc gcagttccgc cagatactcc
     1081 gggcgccggt tcaataccgc ccagccgatt cctgccgtgc tcgcggcttt ggccacctgt
     1141 ggcagggccg tagcccccac gggctccagc agaattgcgt gcggacggga ctcgctcggg
     1201 ccctggatcg ctttcagcag ttgcgtgctc tgattgaccg catcgtcctc ggcaaaaacg
     1261 atctgcacct cgatgcccag cttgcgcgcg gcactttccg cgcattgcgc ctgggccacc
     1321 tggaaatcat tcccggagcc atggagggaa actacaaatc gcagcttctt catcgcgtca
     1381 caaccggact gctcagacgg tattctctaa agatacgctt tgtgccagac gaagtcccct
     1441 gtgccgtcca tgcgtcttgc ggtcgtcaca gcccttgcgt tttgcgattt tccgccggcc
     1501 tgagtgcaat cgcccggttt cgtcaagtga tttccgaatc gacgattcgg taacgcagtg
     1561 acactcgcct accctcacgc tatactctcg taccgctccg cctggcggaa gagattgcct
     1621 ccgtcctcca tcctcggctc cgtcgacgtt cgggcggccc acacccgccg tgtcgtcggc
     1681 ctgcgtctcc ttccttttct cttcgtgctc tacatcgtga actacatcga ccgcacgaac
     1741 ctggcttacg ccgcggtcgg gatgagtcga acgcttggct tcggtgaccg catcttcggc
     1801 ctgggcgccg gcatcttctt tatcagttat ctcgcgctcc agatccccgg cgctcgcatg
     1861 gtagcgcgct tcggcgcccg ccgcgtcatc tcctcctgca tgatcgcctg gggatcgctc
     1921 acagtcctta ctgctctcgt ccatacgccc atccagctct atctcgcccg cttcgtccta
     1981 ggagctgccg aagccagctt tttccccggg gtcatcgtct atctcagcta ttggttcatt
     2041 aatgaagacc gcgccaaagc caccagcaac ttcatgagtg cgattccgct ctcttcgatg
     2101 ttgggatctc ccctggctgg atggattctc ggccgtacgt ggctcggggt tgacggctgg
     2161 cgttggcttt tcgtggcaga gggcttgccg gcgatcgggc tcggcgtcgt cgcttatttc
     2221 tttctcaccg accgcccggg cgacgccaca tggttgcatc ctggcggacg cgactggctg
     2281 ctgcggacgc tcgaggagga gagtccggct cgtaccgcga aagctggttt tcgcgatgcc
     2341 cttcagtcgc ccctcatttg ctttctggcg gctatgactt tcctcgccta cgtcgcgcag
     2401 tacacttttg tgttttggtt cccgctcctc ttcaagcggc tttccgggat gacggacctc
     2461 catgtcggcc tctggggcgc attgccattt ctcggcgctt ttcttctgat gcagatcaat
     2521 ggctggcatt cggacaagat ggcagaacgg cgctggcatg ccgcgatgcc tcttttcctc
     2581 tccggcgccg gcttcctcct gctgacaacg cggcctcagg cgttcccggt gctgcttgct
     2641 ttcttcatga tggcgtcgac tgccacggtt tatttgccga ccttctgggc catcccgccg
     2701 gagatcctca gccccacgat agtcgccacc gcggttggcc tcatcaactc ggtgggcagc
     2761 atcgccggct tcgccgcccc ctatgcgttc ggctatctcc aggccgtcac gggatccctg
     2821 agcgccggcc tttccgtcac cgcggtgtgc gcattcgcgg ccggtttcct catcctgcag
     2881 atcccgaagg gccaggagtg acttgaagcc tggcagcgcc aaattaggct ggaaagggcg
     2941 ggggcgactg tcgaacgcgc taggggtccc cgcaaccctt tgtctctctt cgtttccgct
     3001 tcccgctcgc tgtgtgaccc caatcacaat cccccgcgac ccttgtcact gtcgaaaaac
     3061 taccccccat ccttaaatag aacagcggtt aaaaccgggc gcagagcggc gccggaccgc
     3121 aagattcccg accagaggtt tgagccatgg ccaacgacga cgttgtgaaa tgtccgctgt
     3181 gcggcggatt cacccacatc gagaaccccg atctgctggc agccctgcgg actccggccc
     3241 tgcgcgagca ggtggaacgc ttcatcgccg agcttctgaa gtcgccggtg gaagacctgg
     3301 cctcggtcgg cgccacccag gcgggccgcg atttccagaa agacgtacat aactggaatc
     3361 cctgcgtccc catgtggcgg agaagtccga aggagtagtt tttcgttcgt gaacaggcag
     3421 caggcggaag gggagaccga accgcacgaa ttctccgccc tgccggcggg agaaagggaa
     3481 gagtccatga aagccagaag aatcctgatc ctgggagcag cggggagaga ctttcacaat
     3541 ttcaacgtgg tatttcgggg gaacgccgag tacaacatcg tcggctttac cgcaacccag
     3601 attcccggca tcgccaatcg ccgttatccc acggaactgg ctggcccgct gtatccggcc
     3661 ggcatcccca tcttcgagga gcacgatctg gagcgcctgg tggaagagca cgaagtcgac
     3721 gccgttgtct tttcctatag cgacatctcc caccagaacc tgatgcatct ggcctgccgt
     3781 gtagcggcga caagggcgga cttctggctg ctcagttccg agcgtaccca gctcaaggcc
     3841 tccataccgg tgatttccat ctgcgccgtg cggaccggct gcggcaagag cccgctgggg
     3901 cgcctggtga cctcggaact gcgccgcgaa ggctggaagc ccgtggtgat acgccatccc
     3961 atgccctacg gcgatctggc ggcgcaggcc gtgcaacggt tcgccacatt ggaagacctc
     4021 gacacccagc agtgcactat cgaagaacgc gaagagtatg agccgcacct gcgcgaaggc
     4081 acggtcgtct acgccggcgt cgactatggc cgcattctgc ggcaggccga aaccgaagcc
     4141 gacatcatcg tatgggacgg cggcaacaac gacactccct tctacgcctc cgacctcgaa
     4201 atcgtggtct gcgatccgca ccgccccggc catgagttgg cctactaccc gggcgaagtc
     4261 aatctgcggc gcgctgatgt ggtcgtgatc aacaaggtcg acaccgccgc tccgcaggac
     4321 gtcgaaaccg tgcgccgcaa catccgcatc agcaacccga acgccaaggt ggtggagact
     4381 gcctgccgcg tcgccgtctc tgctcccgaa ctggtgaagg gacgcacggt gctggtcgtc
     4441 gaagatggcc cgaccctcac ccatggcgaa atgccctacg gcgccggagt cgtggcggcg
     4501 cgccagtgtg gagcggccga actggttgac cctcggccct tcgccgtcgg ctccatccgc
     4561 tggacctatg aacactatcc gcatctgacc agcctgttgc cggccatggg atacagcgcc
     4621 atccagcgcc acgaactgga ggagaccatc aaccgcgtgc cctgcgacct ggtgctggtc
     4681 gcaaccccga tcgatctcgg tcacctgatc aagctgaaca agcccagcct gcgcgtgacc
     4741 tacgaggtcg aggatctgac ccatcccggc ctcggggaga ttctggctcg ttttacccgg
     4801 gagcgctcgg gagtgggggt ttaggttacg actctacgct caccacgcgg agtgaccgca
     4861 atcactgctg gagaaccaac cccggcatat acccatcaat gtggattctc tcaagcggcc
     4921 gagccttgtc tagctgagtc tgcggccgca aggaggtacg ccatgtccac caccccgcag
     4981 gtttcccgca ttgccctcaa gaacatactc tttcccaccg atttctctca ggcttcgaac
     5041 ggcgcgctgc ccttcgcgct ctctctggcg cgggtttatg actcgaccgt cgtagtcgca
     5101 catgcgctgc cgcctgagcc gcatctgcag gtggtcctgg accgcgtgcc cgccaccgac
     5161 gagcgcccct ggcacgaagc ccaggacaaa ctcgtccagt tcacccacaa tcgtgcattc
     5221 ggagagattc cctgcaagat cgcccttgat cgcggggatc ttgccgacgt gattccggca
     5281 atgatccgcg aatacgcggc ggacctggta gtgctgggca cgcacggccg gcgcggagtc
     5341 agcaagatcg tgctcggctc cggcgccgag aagatctacc gctccgccag ttgcccggtc
     5401 cttaccgtgg gcccgaaggc gcacgaatct gccgagccct ggacgctgca ccgcatcctg
     5461 tgcccggttg atctggcaga agatcccgaa ccggcgctgc actatgcgct gtcgctggcg
     5521 gaagagcatc aggccgagtt catcgtgatg cagtcgatcc ccctggttcc ctggcagcgc
     5581 cgccctgccg cggaagaaca tgcgcgccgc gacctggaac gcctgattcc ggaatcggcc
     5641 aaagactggt gcgtcccgca atgcatcgtg cgctgggagt atcctgcaga ggcgattctg
     5701 cacgccgcgg ccgagcgcga agtcgacttg attgtgatgg gcgtgcacaa ggcgcgggcc
     5761 gccacttggt ccgcccacct gccctggccg gtggcctcgg aggtggtcag ccgggcgcgt
     5821 tgtccggtgc tgaccgtacg agtttaggtt gcaagcgtcg ctgcgctgct tacacaggca
     5881 gcccgcggcg ccaaggaaat cattggaggc aatcatgcta gtcagcaagc agcaggccct
     5941 cgattatcac tttggggcgc gcccgggaaa gatcgaaatt acacctacca agccgtgccg
     6001 cacgcagcgc gatctgagca tggcgtacac gccgggtgtc gccgagccct gtctcgaaat
     6061 cgagaagaac cctcatgacg cgttcaagta caccggacgc ggcaatctgg tcgccgtcct
     6121 gagcaacgga accgccgtgc tcggcctggg cgacatcggc gccctcgccg gcaagccggt
     6181 catggaaggc aaggcggttc tgttcaaacg ctttgccgat gtcgacgtct tcgacatcga
     6241 ggtcaacagc cacaatccgg acgagatcat caaggtctgc cagctcctcg agcccacctt
     6301 cggcggcatc aacctcgaag acatcaaggc tcccgactgc ttctacatcg aagagacgct
     6361 gcgcaagacc atgaagattc ctgtcttcca cgatgatcaa cacggcaccg cgatcatctc
     6421 gggcgcggct ctgctgaatg cgctcgaagt cgtgggcaaa gatatccgca aggtgaaagt
     6481 ggtcttcaac ggcgccgggg ccgcgggcat tgcttgcgcc gaacactacg tgcggctggg
     6541 cgtgaagcgc gagaacatca ttctctgcga caccaagggc gtcgttcaca aaggccgcaa
     6601 ggaaggcatg aacaagtaca aggaagcctt cgcgatcgag accgacatgc gcactctagg
     6661 tgaagccatg atcggcgcag acgtttttgt cggcctttct gtgaagggcg ctatcaatca
     6721 ggacatggtc cgcaccatgg ctcacgaccc aatcgttttt gccatggcca accccgatcc
     6781 cgaaattact tatgaagacg ccaaggccgc gcgccctgac gtcatcatcg ccaccggacg
     6841 ctcggactat cccaaccagg tcaacaacgt cctcggattc cccttcatct tccggggagc
     6901 gctcgacgtt cgcgcgactg ccatcaacga agaaatgaag ctggcagcga cccgcgcctt
     6961 ggccaatttg accaaggaag atgtcccgga ttccgtctgc cgcgcctacg gcgtgcagcg
     7021 cctgcagttc ggccgcgagt acctgattcc caagccgttc gatccccgcg tgctgatgtg
     7081 ggaggctgcg gccgtcgcag aagccgccat gcgcacgggc gtggcgcagg aaccggtcga
     7141 tctcgaaaat taccgcgccc agctcgagcg aagattgggc aaggcctacg aggtctcgcg
     7201 caccatgatt cacaaggccc agatcaatcc caagcacgtg gtctttcccg aaggcgacaa
     7261 cgagaagatt ctgcgtgctg cgcacagtct cgttgacgag aagattgctc atcccatcct
     7321 cctcggcgac gctgagaaga ttcgtgcgcg ggcccatgag ctggggctcg atctggaggg
     7381 catgcagatt gtcgatccgg caacgtccga ccaccgcgag tcctacgcgc tcgagctctt
     7441 caacctgcgc cagcgccgcg gcgtgacgct cgaagaagcg cacacgctga tcggcgaccg
     7501 caacgttttc ggctccatga tggtgcgcat gggggatgcc gatgcgctgg tctccggcgt
     7561 cacccagcat ttctccgaga ccatccgccc ggcgttgcag atcgtgcgca tgcgcgaggg
     7621 tctgcatcgc gtctccggct gctacgccat gatcacgcgc aagggcgagg tctttttcct
     7681 cgccgacacc agcgtgaaca ttgaaccgac cgccgaagac ctggtcgaaa ttgcgctgtg
     7741 caccgcccac atggcgcggc gtttcgacat gagcccgcgt gtggccatgc tgtcgttctc
     7801 cagctttggc agcgtcgacc acccgatctg tacgaaggtt cgtaaagcgg tcgagttgct
     7861 gcaccaggct gaccccaccc tgattgtcga cggtgaaatc gccgctgacg atgccgtgtc
     7921 tccggaagtc atcgagcagg aatatccgtt cagcgccctt aagggtggcg ccaatgtgct
     7981 catcttcccc gacttggcgt cggctaacgt ggctaccaag ctgctcgcca agatcggcgg
     8041 cggcgaggtc attggcccca tcctgatggg catgagccgc ccggtgcatg tgctgcagcg
     8101 cacggccaca gtggaagaga ttgtcagcgt ggctgccatc gctgtcgtgg acgcgcagga
     8161 acacgacatc tatgcccacc cgcacgtgca tgtatcggtc gagccggaac tggtggggga
     8221 agactgataa gcttgtggag ggccacggtt ctgcggcttg aaaagggcac ggctttcggg
     8281 gtcgaacaga ttcaaaagcc tcaccgtgca agagcggccc tttagggccg cgtcagctgc
     8341 gcttcaatac ggggctttag cccctgaggc actcatccat gtcgacactc tggaagtccc
     8401 gctacgcgca acgcacgttc aacatcaaga gctccgccat ccgtgagctt ctcaaggtca
     8461 cgcagcgccc cgagattatt tcctttggcg gaggcctccc cgcacccgac gtctttcccg
     8521 tcaagcgctt cgaagaagcc tgccacaaag tcctgaccga acacggcgcc caggccctgc
     8581 aatatggccc taccgagggc tacccgccgc tgcgcgagat ga
//
LOCUS       DASPUL010000637         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1349580, whole genome shotgun sequence.
ACCESSION   DASPUL010000637 DASPUL010000000
VERSION     DASPUL010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349580"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            463..1389
                     /gene="mazG"
                     /locus_tag="VEJ00_14705"
     CDS             463..1389
                     /gene="mazG"
                     /locus_tag="VEJ00_14705"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HXX02464.1"
                     /translation="MPTTGERFERAVAIMDRLRAPGGCPWDREQTFDSIKPYTLEETY
                     EVLEAIDNRDWPELAGELGDLLLQILFYSEMAKEQGTFSIDEVLDRLSAKLIGRHPHV
                     FGDVKADTSAEVKLNWEALKVEERKQREDAQESGRKHDAGIGQRSSILANISSAMPSL
                     LEAYKLSSRAAQTGFDWPDIEGLFDKLHEETNELREHLKEFPAPGPRPQGRGVAGSGR
                     TVIPDDLQVKLEEEVGDLLFVLVNVARYLSLDPESALRKTNRKFRRRFQWMEERLHES
                     GRSADDVPMEELESLWQQAKSAEQKGKSIATD"
     gene            1402..2175
                     /locus_tag="VEJ00_14710"
     CDS             1402..2175
                     /locus_tag="VEJ00_14710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX02465.1"
                     /translation="MSSDSIVLRRCHSLDEMRACVALQKEVWNFSDAELVPLRMFVVA
                     DKVGGQVMGAFDGNTMVGFALSVPGTRTGHVYLHSHMLAVRKDYRNSGLGRRLKLMQR
                     EDALARGIELIEWTFDPLEIKNAYLNLEKLGAIARRYNINQYGITSSPLQGGLPSDRL
                     IAEWWLKSKRVETLLATGKNPPFRRELEIVVPAKIYEWKATAETRGNAQQVQEHNREQ
                     FLRAFAQGLAVLGYDRDAAGNGKFLLGHWDETWSYASQP"
     gene            complement(2257..>2645)
                     /locus_tag="VEJ00_14715"
     CDS             complement(2257..>2645)
                     /locus_tag="VEJ00_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02466.1"
                     /translation="ELTASVVANLSQPANQKKLGIQEIFAGNSLKNKWDDPGTDPRTP
                     DIVLKVNTGVIFTGGSKIAEHGGLNEDDVHVALLVSFAGLQPQSVRTAVLNQQVAPTI
                     IKALGFDPTQLQAVQQEGIQPLPFLF"
BASE COUNT          548 a          791 c          801 g          505 t
ORIGIN      
        1 acgccggagc cgaagtcgtt ttgctcaacc agcaaggtgc gcttgccggc acgggcacac
       61 tcgcgggcca cggccacgcc gttgatgcca ccgccgatga ctgcgacctg gaagtgctgg
      121 ccttccaaag gtggtcgagg cttagctgcc aaagtcattt gtttcagtta gatgcgtggt
      181 cctcgcgaag tgtcgctcga aggcggtcac acacggagct gcccctcgcg gcacgcctca
      241 tactttagtt tgcgcccttc ccggcgaggg cagtaggtca cgtaagtgcc agaagatcgt
      301 ttcaccgatg ctgcggaagt ggctcgatca aacgtcactc tggcctggtg actagagtac
      361 ttcactgcaa attgcttgac tccacgtgaa ctcgcgccgg ttctgtgcac catccttcac
      421 gcggcattgc ccgtcgcacg aaccctcgtt tagagtgttg ccgtgcctac aaccggcgaa
      481 cgatttgaac gtgcggtcgc gatcatggac cgattgcgcg cccctggcgg ctgtccctgg
      541 gaccgcgaac agaccttcga ttccatcaag ccctatacgc tggaagaaac ctacgaggtc
      601 ctggaagcca tcgacaaccg cgactggcct gaactggcgg gagaactcgg cgatctgctc
      661 ttgcagatac tgttctattc cgagatggcg aaagagcaag gcacgttttc catcgacgag
      721 gttctcgatc gcctctccgc caagctgatt gggcgccacc cgcatgtctt cggcgatgtg
      781 aaggctgaca cctccgccga agtcaagctc aactgggaag ctctcaaggt cgaagaacgt
      841 aagcagcgag aggatgccca agaaagcggc cgcaagcatg atgccgggat tggacagaga
      901 agttcgatcc ttgcgaatat ctcctccgcc atgccgtcgc tgctcgaagc ctacaagctc
      961 agttcgaggg ccgcgcagac aggcttcgac tggcctgata tcgaaggctt atttgacaag
     1021 ctccacgagg agaccaacga gctgcgcgag cacctgaagg aatttcccgc gcccggtccg
     1081 cgcccgcaag gccggggcgt agccgggtca gggcgaacgg tgatccccga tgaccttcaa
     1141 gtgaaacttg aagaggaagt aggcgatctg ttgtttgtcc tcgtcaatgt tgcacgctac
     1201 ctttcgctcg atccggaatc ggcattgcgc aaaacgaacc gcaagttccg ccgccgcttt
     1261 cagtggatgg aagagcgact gcacgagtcg ggtcgctcgg ccgacgatgt ccccatggaa
     1321 gagctcgagt ccctttggca gcaggccaaa tcggccgagc agaaggggaa atccattgca
     1381 acagattgag gagaagcagg cgtgagctcc gacagcattg tgctccgccg atgtcacagc
     1441 ctcgacgaaa tgcgggcgtg cgtcgctttg caaaaggaag tctggaactt cagcgacgcc
     1501 gaactggtgc cgttgcgcat gttcgtggtg gccgacaagg ttggaggcca ggtcatggga
     1561 gctttcgacg gcaacaccat ggtcggcttc gcgctctcgg tgcccggcac gcgcaccggc
     1621 cacgtctacc tgcactcgca catgctggcc gtgcgcaagg attatcgcaa cagcggtctc
     1681 ggccggcggc tcaagctgat gcagcgcgaa gatgccctcg cccgcggcat cgagctgatc
     1741 gaatggacct tcgatccgct ggagatcaag aacgcttacc tgaaccttga aaagctgggc
     1801 gctatcgcgc gtcgctacaa catcaaccag tatgggatca cctcgtcacc gctgcagggc
     1861 gggctgccca gcgaccggct gatcgccgag tggtggctga aatcgaaacg cgtcgaaacg
     1921 ctgctggcga cggggaagaa cccgccattc cgacgcgaac tcgagatcgt ggttcccgcc
     1981 aagatctacg agtggaaagc cacagccgaa actcgcggca atgcgcaaca ggtgcaagag
     2041 cacaatcggg agcagttcct ccgtgcgttc gcgcaaggtc tcgcggtctt gggctatgat
     2101 cgcgatgccg cgggaaacgg caagttcctg ctcgggcact gggatgaaac ttggtcgtat
     2161 gcgtcccagc cctgatcgcg gcgagcgcac cgactgggaa caaagagaaa agcggcggag
     2221 ctttccggtc cgccgcccag cttaacgtcc aaccgctcaa aacaggaacg gaagcggctg
     2281 aatcccttcc tgctgtacag cctgcaactg ggtgggatcg aagcccagcg ccttgatgat
     2341 ggtgggtgcg acctgctggt tcaggacggc tgtcctcacc gattgcggct gcagtccggc
     2401 gaacgacacc agcagagcca cgtgcacgtc gtcttcgttt aggccgccgt gctcggcgat
     2461 cttagacccg ccagtgaaga tcactcccgt gttgaccttg agaacaatgt caggggtacg
     2521 cgggtcggta cccggatcat cccatttgtt cttcagcgag tttccggcga agatctcctg
     2581 gatgccgagc tttttctgat tggcgggctg gctaaggttc gccaccacgc tggcggtgag
     2641 ctcct
//
LOCUS       DASPUL010000638         4619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1220530, whole genome shotgun sequence.
ACCESSION   DASPUL010000638 DASPUL010000000
VERSION     DASPUL010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220530"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            363..2051
                     /locus_tag="VEJ00_14720"
     CDS             363..2051
                     /locus_tag="VEJ00_14720"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXX02467.1"
                     /translation="MQRLATACEAIAATTKKLQKIAIVADYFKLRTVEEAAVSAVFLS
                     GPAFPAWEETTLQIGSRSLWRIVAELAGKGEGDLTAAYRRLGDLGAVARDVLPQRPGQ
                     GIGLLEVSEGFQQIAAARGPAAKTALVHDLLSRATPLEAQYIVKIMTGDLRIGLKESL
                     VEEAIAKAYGGTLAEIQRANMLLGDIGETLRLAVGGELADARLRMFHPLGFMLASPID
                     SAEEGLSYFSEAAVEDKYDGIRAQAHVSKGEVRLFSRTRDEITESFPEMPAPLARLPY
                     DAILDGEIVAWDYQKQKARPFSILQQRLGRKKVSEKMLWELPVAYLAFDVLYAGGELL
                     IDHPLWERARILDELLARRRVHPITERGSAGQQQFAFSDDRPLVADVIRSPLSRAGSP
                     SELEELFSAAQERGNEGLMIKDLNSSYTPGKRGKSWLKIKRELATLDAVVTAVEYGHG
                     KRIGVLSDYTFGVWDGDRLVNIGKAYSGLTDTEIAEMTSWFLEHTIEDQGFRRVVEPK
                     IVLEVAFNNMMRSDRHESGFALRFPRIVRLRPDKSPEEADTLDRAKEIYEKQQR"
     gene            complement(2061..2990)
                     /locus_tag="VEJ00_14725"
     CDS             complement(2061..2990)
                     /locus_tag="VEJ00_14725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXX02468.1"
                     /translation="MHVHAHGAGPTRVLKISLGVTLAYIVLLVVAGIRAHSLALLSEA
                     GHNLSDFLALLLSLVAVYFQSRPANASKTYGYQRAGVLAALVNATSLVAVSFFIFYEA
                     FRRLQHPEHVQASVMMWVAAAGVVMNGVIALLLYRSGGDVNIRSALLHEVGDTLSTAA
                     VIAGGWAILITGNYWIDSALSVGIGALILWSAFGIVRETLNILLEGTPRGMKLEKVES
                     AIREIEGVNDVHDLHVWSIGSESHALSCHISIADIPPSVSERILRDVKECLLHDFRID
                     HTTIQFEHIVCEVAHGCVIPVAENEEEHHRHSH"
     gene            complement(3059..3925)
                     /gene="gluQRS"
                     /locus_tag="VEJ00_14730"
     CDS             complement(3059..3925)
                     /gene="gluQRS"
                     /locus_tag="VEJ00_14730"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HXX02469.1"
                     /translation="MASNSVDPVKLYRGRLAPSPTGLLHLGHARTFWIAAQRAHDRDG
                     TLILRNEDLDAQRCRTEFVDAMLEDLRWLGITWDEGPDCGGPLGPYRQSERRQHYLKA
                     WKCLNDQGMIYPCTCSRKDVAQAVAAPNDSDDEPMYPGKCRPGRTAPRFQAEPAGVNW
                     RFRVPDGEEIRFTDLHLGPQAFIAGRDFGDFIIWRRDDVPAYQLAVVVDDAAMRITEV
                     VRGADLLKSTARQILLFRALGVPVPDYYHCDLVHDAAGVRLAKRHDALSIRKLRELGG
                     TPEQVRTRAQAL"
     gene            complement(3935..4618)
                     /locus_tag="VEJ00_14735"
     CDS             complement(3935..4618)
                     /locus_tag="VEJ00_14735"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXX02470.1"
                     /translation="MKFCDEIAAAGFAILPERVSRQCVDSLLNQVDQRSTRRTRAGVR
                     HAMGLRPVAALTRHTELMQLARQVLGQDAFPFRATLFNKSPESNWLVVWHQDTALPLQ
                     GRSELPGWGPWSVKEEIHYAHAPASALSQVLALRVHLDDSTRDNGPLRVLPGTHQLGV
                     LTDDAIHNLARRIDPVECVVAKGGVVAMRPLLIHASSKSQAALPRKVLHIEYAASASI
                     AEPLQLAIA"
BASE COUNT         1013 a         1359 c         1378 g          869 t
ORIGIN      
        1 ctcatggtaa ccacttcgcc agagggcttg cggcaggcga tggcccacgc gtgcggggac
       61 cgcatcatca cgccctccaa cacggcctgc ccgccaacca gggtctcctc gccgctttca
      121 agggcaggca gtagttgcac cgcagccaga aatttcaggt aatttcgcag aaaaggcaca
      181 gagttttaga tgatccttcc gagggaagcg caccaatgta gtaggttatc acaggtcgcg
      241 aaatggtttc gagaacggtg ggagccgcgc gaactctatg cgttagtggg acttagatct
      301 tacagagcgg gttagcgtcg agagtggaag tgctccgccg gacagccctc atccaagaat
      361 ttatgcaacg actggcgaca gcttgtgagg cgatcgcagc cactacaaag aagctgcaga
      421 aaatagcgat cgtggcggat tatttcaagt tgcgcacggt cgaagaggcg gctgtgtcgg
      481 cggtctttct ctccggacca gcatttccag cctgggagga aacaactctc caaatcggca
      541 gcagatcgct ttggcggatt gtagcggagc tggcaggaaa aggcgaaggt gacctgaccg
      601 ccgcctatcg ccggcttggc gacttaggag cggtcgcgcg cgatgttctt ccgcagcggc
      661 cgggccaggg gattggcctt ctggaggtaa gcgaaggttt tcagcagatc gcggcagccc
      721 gcggaccggc tgccaagacg gccctcgtcc acgatctgct gtcccgagca actccgctgg
      781 aagcccagta tatcgtcaag attatgaccg gcgacctgcg aattggtctc aaagaaagcc
      841 tggtggaaga agcgattgcc aaagcctatg gcggcactct ggcggagatc cagcgcgcca
      901 acatgctgct cggcgacatt ggcgagacct tgcggctcgc ggtggggggc gagctggccg
      961 atgccaggct gcggatgttt caccctctcg gattcatgct ggccagcccg attgactcgg
     1021 cagaggaagg actgagttac ttctccgaag cggccgttga agacaagtac gacggcatcc
     1081 gggctcaggc acatgtttct aaaggcgagg tccggctttt ctcccgcaca cgggatgaga
     1141 tcactgaatc gttcccagag atgcccgccc cgctcgcccg gctgccgtac gacgcgattc
     1201 tagacggcga gatcgtcgct tgggattacc agaagcagaa ggcccgaccg ttcagcatcc
     1261 tgcagcagcg gctcggaaga aagaaagtga gcgagaagat gttatgggaa ctccccgtcg
     1321 cctacctggc gtttgacgtg ctgtatgcgg gaggcgaact cctgatcgac catccgctgt
     1381 gggagcgagc acgaatcctg gacgaattgc ttgcgaggcg aagagtccac cctattacag
     1441 agagagggag cgcggggcaa caacagttcg cgttcagcga cgataggccc ctggtggccg
     1501 acgtcattcg atcgcctctg tcgcgcgcgg gatcgcccag tgaactggaa gaacttttct
     1561 ccgcggccca ggagcggggc aacgaaggcc tgatgatcaa ggatctcaac tcgtcttaca
     1621 cgccggggaa gcgcggcaag tcctggctaa agataaagcg cgagttggcc acgctcgacg
     1681 cggttgtgac cgcggttgag tatggtcatg gcaagcgaat cggcgtgctc agcgactaca
     1741 ccttcggagt atgggatggc gaccggttgg taaacatcgg caaagcctat tccgggctga
     1801 ccgataccga gattgccgag atgacgagtt ggtttctcga acacaccatc gaagaccagg
     1861 gcttccggcg tgtggtcgaa ccgaagattg tgctggaagt ggcgttcaac aacatgatgc
     1921 gctccgaccg ccacgagagt gggtttgcac tgcgattccc gaggatcgtc cgtttgcgtc
     1981 cggacaagtc accggaggaa gccgatactc ttgatcgggc aaaggagatc tacgagaaac
     2041 agcagagata gaatcgcgct tcagtggctg tgacggtgat gctcctcctc gttctccgcc
     2101 acaggaataa cacaaccgtg ggccacttcg cacacgatgt gttcgaactg aatcgtggtg
     2161 tgatcaatcc gaaaatcgtg cagcaggcac tccttcacgt cgcgcagaat tcgctcgctc
     2221 accgagggag gaatatccgc aatcgaaatg tggcaggaga gcgcatggct ttctgatccg
     2281 atactccaga cgtgcagatc atgcacatcg ttgactcctt cgatctcccg tatcgccgac
     2341 tcgaccttct ccagcttcat gccgcgtgga gtgccttcaa gcagtatgtt gagcgtctcc
     2401 cgcacgatgc cgaaggccga ccacaggatc aaagcaccaa tcccgaccga gagggcggag
     2461 tcaatccagt aatttccggt gatcagaatg gcccagccgc ctgcgattac ggccgctgtc
     2521 gaaagcgtgt cgccgacttc gtgcaaaagg gcgctgcgga tgttgacgtc gccgccggag
     2581 cggtacagca acagtgcaat cactccattc atcacgacgc ccgcggccgc gacccacatc
     2641 atcacactgg cctggacatg ctcggggtgc tgcagccggc ggaacgcctc atagaaaata
     2701 aagaaagaga cggcaaccag agaggtcgca ttgacgagcg ccgccagaac tcccgcacgc
     2761 tggtatccgt aggtctttga agcattggcc gggcgcgact gaaaatagac ggcaaccagc
     2821 gacagcagaa gtgcgagaaa gtcggataga ttgtgccccg cttcagacag cagcgccaaa
     2881 gaatgcgccc ggatgccggc taccaccaac agcacgatat aggcgagagt caccccgagt
     2941 gagatcttca gcactcgcgt ggggcctgcg ccgtgggcgt gcacgtgcat tctgtaagtc
     3001 tacgggccgt gatggcggcc ttcaagcggc tttgcggccg acgcatccta cgctggattc
     3061 aaagtgcttg cgcccgggtc cgcacttgct ccggcgtccc tcccaactcg cgcagtttcc
     3121 ggatgctcag cgcatcgtgg cgcttcgcca gccgcacgcc cgccgcgtcg tgcaccaaat
     3181 cgcagtggta gtagtccggc accggcactc ccagcgcccg gaacaacaga atctggcgag
     3241 ccgttgactt cagtagatct gccccgcgca ctacttcggt gattcgcatc gcagcgtcgt
     3301 cgacaactac ggccaattgg taagcgggca catcgtcccg cctccatatg atgaagtcgc
     3361 cgaaatcgcg ccccgcgatg aaggcctgcg gtccaagatg cagatcggta aagcgaatct
     3421 cttcgccgtc agggacgcga aagcgccaat tcacgcccgc gggctcggct tggaaccttg
     3481 gcgccgtacg accgggacgg catttccccg ggtacattgg ctcgtcgtcg gaatcgttcg
     3541 gcgctgccac cgcctgcgct acatcctttc gcgagcaggt gcatggatag atcattccct
     3601 gatcgttcag acatttccat gccttcaagt aatgctgccg ccgctcgctt tgcctgtacg
     3661 gaccaagtgg ccctccgcag tctggccctt catcccaagt gattcccagc cagcgcaagt
     3721 cctcgagcat ggcatcgaca aactcggttc ggcagcgctg cgcgtccaga tcttcattgc
     3781 gcagaatcaa ggttccatca cgatcgtgtg cccgctgggc cgcgatccag aatgttcggg
     3841 cgtgtcccag atgcagcaag ccggtcggcg acggcgctag gcgcccgcgg taaagcttga
     3901 cgggatcaac agaattcgag gccatcgctc gccgctaagc aatcgccagt tgaagaggct
     3961 cagcaatcga cgctgaagcc gcgtattcga tgtgcagaac ttttctaggc agggcagcct
     4021 gtgacttgga agacgcatga atcagcaacg gcctcattgc tacgactccg ccctttgcga
     4081 ccacgcattc gaccggatca attcgtcgcg caagattatg aatcgcatcg tccgtcagca
     4141 caccgagttg atgtgtgccg ggcaaaaccc taagtgggcc gttgtctcga gtcgagtcgt
     4201 caaggtgaac gcgcagagct aatacttgag agagcgctga tgcgggggcg tgtgcgtagt
     4261 gaatctcttc tttgaccgac catggccccc atccaggcag ctcagaacga ccctgcaaag
     4321 gcaaggccgt atcctgatgc caaacaacga gccaattgga ttccggagat ttgttgaaca
     4381 acgttgcgcg gaacggaaag gcgtcctgcc ccaggacctg acgagccagt tgcataagct
     4441 ccgtgtgtcg ggtcagggcc gcaactggac gaagtcccat agcgtgtcga acccctgcgc
     4501 gggtacgccg cgtggaccgc tgatcaacct gattcagaag ggaatcgaca cattgccgcg
     4561 aaacacgttc cggcaggatg gcaaacccgg ccgcggcaat ctcatcacaa aacttcata
//
LOCUS       DASPUL010000639         9395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_266996, whole genome shotgun sequence.
ACCESSION   DASPUL010000639 DASPUL010000000
VERSION     DASPUL010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9395)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9395
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266996"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1865)
                     /locus_tag="VEJ00_14740"
     CDS             complement(156..1865)
                     /locus_tag="VEJ00_14740"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXX02471.1"
                     /translation="MTPASTESRRQQLLSLYEEEWQYELRTSPEFATVLGDTRYNDRL
                     SDNSPEFFRSDLEQKQKFLSRFQAIDPAGLPHQDKLSLELMIRQLNQEIEGAQFKSWE
                     MPVNQMGGIHLELPDLVELTPFNMVTDYENYLARLRQIPRAFDQVTANMRQGMRDGLM
                     PPRYLLQKVAAEADDVASKTGEDSPFAKPVKTFPESVSAADRKRLRDAILAAIVEQIT
                     PAYRRFAIFVRDEYAPHGRTDPGVWTLPDGAARYRFAIRRLTTTDMTAAEIHALGLKQ
                     VDEIEAEMLAVAHKLGFNDLASLNDHIKSERSFYAASGQQLLDLYTNYARNMEPELPK
                     LFGHLPKSKLTVIPMEASRSKNAVPADYTDGAADGSRPGHINVNESDPEHRLVLNVEA
                     IAYHEGIPGHHLQLSLAQEMQDLPQFRRHAGYTAFVEGWALYAERLGKEVGHYQDPYS
                     DYGRLENEMWRAIRLVVDTGVHDMHWTRQQMVDYFHRYTAMDEPNVQSEVDRYIAWPG
                     QALAYKLGQLEILKLREKGHAKLGDKFDIRAFHDEVLGNGALPLDVLDSEVGSWIDTQ
                     SKQ"
     gene            complement(2019..3848)
                     /locus_tag="VEJ00_14745"
     CDS             complement(2019..3848)
                     /locus_tag="VEJ00_14745"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HXX02472.1"
                     /translation="MDTTHKDGKPVSYEKVPWKARPFPMKQVKLGDGPCRVATEADRR
                     YLHSLPPDRLLHTFRSNAGLPSSAQPLGGWEAPDCELRGHYAGGHYLSACALMYASTG
                     DEDLKNNASLVVSELGRCQAALKSGYLSAFPIEFFDRLRERERVWAPFYTIHKIMAGL
                     LDMYVYCGDEQALDMVQKMAGWVAGYTGSLSYEHMQRVLGTEFGGMGEVLSNLYAVTG
                     KEYYLHVAQRFDKKQFFDPLAAHRDELKGLHVNTHIPQVIAAARYYELTGDRRYRDIA
                     EYFWDEVVSERSYCTGGTSNGESWNTDPGKLSTELSTNTTECCCAYNMMKLTRHLFGW
                     SPDARFMDYYERALFNHRLGTINPEDGTMMYYLPLAAGYWKTFGKPFDSLWCCTGTGS
                     EEYAKLTDTIYFHDDDSLYVNLYIDSQLEWPEKGLGVKQSTRFPEEPGTTLAITGKGP
                     TQLAINLRIPYWATGGSVKINGEALPAFSSPSSYLTLNRVWKPGDKIQLSLPMRLHID
                     SMPDDATEQAAMYGPLVLAGRFEAVTREMTYGDLEPKPGNQRKVANITADPKEPTAWI
                     EPDARQPLTFHTVGQAETSTLVPLYQVIHERYAVYWKVDKRST"
     gene            4328..7705
                     /locus_tag="VEJ00_14750"
     CDS             4328..7705
                     /locus_tag="VEJ00_14750"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXX02473.1"
                     /translation="MPRIRSLVRVLLTILAALTWVTCTAAAQQITGNIQGTVNDPSRA
                     VVQSAVVTVEQIETGLSRSAVTDRGGRYLLLELPVGHYRLEVAARGFRKYLQEGISLD
                     VNETATVHVHLQVGSETQQIRVDANAALIQNTVTGLGQTVMEHEILDLPLDGRDFAQL
                     GILQPGVVPLTPGLIQAGGGLRDNQGYAVDGQRPESNNFLIDGADNVNNVDAGFVLKP
                     PIDAISEFRIITHNADAEFGRNTGSTTNIITRSGSNSFHGAAWEFLRNDAMDGSDYFT
                     RTVQPLKQNQFGATFGGPIVRDKTFFFAYYEGFRNRQGETDSATVPSLPERQGNFQEL
                     CTNRPLNGQFAGGVCTDSKGNFLPRGQLFSLFTGQPIPVPNNMLTGPFDSTAQNVMPF
                     FPQPNSGTNTFITTQTKRETNDQVGVRIDHYLSRSDSLNFRYMFSGGPTFDPLSPAGA
                     NVPGFPIGADDRAQNFVAQESHTFSASVIAVARFSYLRNKFLFGDQINHESPTQLGFQ
                     YEPSLGSAAGPPFIQVGGYASVGDPITGPRNTFQNTFDYSGSLSWVHGAHDLKFGGGY
                     RHDGINVLQGIATNGFFVFAPFPYSDGFASFLSGVPVFFLQGVGNFSRGIRGQASNVY
                     AQDTFKVTPRLTMNYGLRYELPSPDTEIHNRQNLFIPGAQSKVFPGAPAGLLYPGDPG
                     VPAGLISTDRKGLAPRMGVAWDPTGSSRWLLRAAYGIFYEPYYTGQGGPLQAPISAPP
                     YIQTPQVNGPQSFANPYLNQSLFNGPFAEPMTLLTLAPKLSLPYAQDWNLTIQKSFGD
                     NWLAEVGYVGTNGSKLPRFIEGNPTTFVPGVDSRGNPISNENNVNQRRLYSGCTLAQP
                     NGCVYASAGLIAGVANSSYNALEASLRKRFSHGLSFLASYTLSKSIDDVSSFNITGSA
                     SQPVAGENDLAQNPFDLSAERGRSMFDARHRFVLSYQWNLPFWNRPSGWYEHVLGNWQ
                     LNGIATLMSGTPFTVFDSSDNSLQGGAPEITGFSANRPNLIANPNSGPRTPQEWMNVN
                     AFQQLAPDPLGRFQVFGNEGRNVVEGPAYLNWDFSAFKNIPISESREFQFRGELFNFL
                     NHTNFRLPVSDISSPNFGQIQQDVGPRVIQVALKFLF"
     gene            complement(7797..9122)
                     /locus_tag="VEJ00_14755"
     CDS             complement(7797..9122)
                     /locus_tag="VEJ00_14755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX02474.1"
                     /translation="MGTIPQDSSPARPPIRHLRWWIGALLFASTVINYIDRQTLANLA
                     PYLKQTYHWTNTDYANIVIGFRVAYSIGQTLCGRGMDRVGTRRGLSLSVAWYSLVAVA
                     TSLARGFYSFAGFRFLLGAGESANWPGATKAVSEWFPKRERGLATALFDSGSSIGGAI
                     APFIVLKVYFHWGWRPAFVIPGLLGFLWLYVWRRSYRPPEEHRRISPEELEMILQDRD
                     DPEITAKGSKPRWRDLLRLPQTWGAIFAKTLTDPVWFFVTDWFPIYLVAKGISLQSGL
                     IAVWVPFIAADLGNFFGGGASGYLIKRGWTVGAARKALVVFGACGVLLLIPTVLTANV
                     WAITLLFALSTFSYASFSTIANVLPADLFLSDSVASVSGMSGTGAGIGTIVAFKLIGY
                     LSDARQAQATHSFDPIVITAGLVPFAGMILVLLLVRNTRATAQGMVRPI"
     gene            complement(9191..>9395)
                     /locus_tag="VEJ00_14760"
     CDS             complement(9191..>9395)
                     /locus_tag="VEJ00_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02475.1"
                     /translation="RQILKARQIIAPVPEARKAKAVKACLEGDISPLAPASALRMHPN
                     VTVFLDLQSAALLNSRQQVQAQR"
BASE COUNT         1895 a         2835 c         2678 g         1987 t
ORIGIN      
        1 gtgcggtaag ggaacgaatc cgctgcgccc aacatgcctt tgacgccaag agttgttacg
       61 accgttgtgg ccgcagcatt cttcaggaat tctcttcgat ccatagagtc gctccttggc
      121 tcccggcgct cggtcgagac gcgcgcggtt gaaaactatt gtttggattg tgtgtcgatc
      181 cagctcccca cttccgagtc cagcacatcc agaggcaagg cgccgttgcc cagcacctca
      241 tcatgaaacg cgcggatgtc gaacttgtcg cccagcttgg cgtgcccttt ctcgcgtagc
      301 ttcaggatct ccagttgccc gagcttgtac gccagggcct ggccgggcca cgcgatataa
      361 cggtcgactt cactctgcac gttcggctcg tccatcgccg tgtagcgatg gaaataatcc
      421 accatctgct gccgcgtcca gtgcatgtcg tgaacgccgg tgtcgacgac cagccggatc
      481 gcgcgccaca tttcgttctc cagacggccg tagtcgctgt acggatcctg ataatgtccc
      541 acctcttttc cgagccgttc cgcgtagagc gcccagccct ccacaaatgc ggtgtaaccg
      601 gcgtgccgac ggaactgcgg cagatcctgc atttcctgcg caagagaaag ttgcaggtga
      661 tgtccgggga tgccttcgtg gtaggcaatc gcctcaacgt tcaacaccag gcgatgctcg
      721 gggtccgatt cgttgacgtt gatatgcccg ggccgcgagc catcggcggc gccgtcggta
      781 tagtccgcgg gcacggcgtt cttggaccgc gaggcttcca tcggaatgac tgtaagcttg
      841 cttttgggaa gatggccaaa cagcttagga agttccggct ccatgttgcg agcgtagttc
      901 gtgtaaaggt cgagcagttg ctgccccgag gccgcgtaaa aactgcgttc gctcttgatg
      961 tggtcgttca gagacgccag gtcgttgaag ccgagcttgt gagcgacggc cagcatttcg
     1021 gcttcgatct catcgacctg cttcaggccg agggcatgaa tctccgccgc ggtcatatcc
     1081 gtggtagtca agcggcggat ggcgaagcga tagcgcgccg ctccgtcggg cagagtccac
     1141 actcccggat cggtgcggcc gtgcggtgcg tattcgtccc tgacgaagat ggcgaagcga
     1201 cggtaggcag gggtgatctg ctcgactatc gccgcaagaa tcgcgtcccg cagtcttttc
     1261 cggtccgccg cactcacgct ttccggaaaa gtcttcaccg gcttggcgaa cggactgtcc
     1321 tctccggtct tgctcgccac gtcgtccgct tcggcggcta ccttctgcag caggtagcgc
     1381 ggcggcatca gtccatcgcg catgccttgc ctcatgttcg ccgttacctg atcgaatgca
     1441 cgcgggatct ggcgcaggcg cgccagatag ttctcataat ccgtgaccat gttgaaaggc
     1501 gttagctcca ccagatcggg caattcgaga tggattccgc ccatctggtt cacgggcatc
     1561 tcccaggact tgaactgcgc gccttcgatc tcctggttca attggcgaat catcaactca
     1621 aggctcagct tgtcctggtg cggaaggcca gccggatcga tggcctggaa gcggctcaga
     1681 aacttctgtt tctgctcgag atcggatcga aaaaattctg gagagttgtc gctcaggcga
     1741 tcgttgtagc gggtatcgcc cagtacggtt gcaaattcgg ggctggtgcg cagttcatat
     1801 tgccactcct cctcatacaa ggagagaagt tgctgccgcc gactttcggt tgaggcggga
     1861 gtcaatgagt tgttctgacc agatgagagc tggtcgagtg tacccgccag aagacagatc
     1921 tgcacaccgc aaatgaacaa tggtcggact ggaaatatca tcggtgcccc agcgagtgat
     1981 tgtgaccgcc accgccaagc cggcggtgaa gaaaatcgtt acgtagatct cttgtcgact
     2041 ttccaataga ccgcgtagcg ctcgtgaatg acctgataca gaggcaccag tgtcgaagtc
     2101 tctgcttgcc ccacggtgtg gaaggtcagc ggctgtcggg catcgggctc gatccatgcc
     2161 gtcggctctt tggggtcggc ggtaatgttc gccacctttc tctggttgcc cggcttcggc
     2221 tccaggtcgc cgtacgtcat ctctcttgtg accgcctcga atcgtcctgc cagaaccagc
     2281 ggcccataca tcgccgcctg ttcggtcgca tcgtcgggca tgctgtcgat gtgaagcctc
     2341 atgggcaggc tcagctgaat tttgtcgccg ggcttccaca cacggttcag cgtgaggtag
     2401 ctgctcgggc tcgaaaacgc cggcagagcc tcgccgttga tcttcacgct gccgccggtt
     2461 gcccaatagg gaatacgcag gttgatcgca agctgagtgg ggccctttcc agtgatcgcg
     2521 agcgtcgtcc cgggttcttc ggggaaacgt gtactctgtt ttacaccaag acccttctcg
     2581 ggccactcca gctgggagtc gatatagaga ttcacataca gcgaatcgtc gtcatggaaa
     2641 tagatcgtgt ccgtcagctt ggcatattct tccgaacccg tgcccgtgca gcaccataga
     2701 gagtcaaagg gcttgccgaa agtcttccaa tatcccgctg ccagcggcag gtaatacatc
     2761 atggtgccgt cttccggatt gatcgtgccc agcctgtggt tgaacagtgc gcgctcgtag
     2821 tagtccataa agcgggcgtc gggcgaccag ccgaagaggt gccgcgtcag cttcatcatg
     2881 ttgtaagcgc agcagcattc cgtggtgttg gtgctgagct cggtggaaag tttgccagga
     2941 tccgtgttcc acgactcgcc gttgctcgtg ccgccagtgc aatacgagcg ctcgctgacg
     3001 acttcgtccc agaagtactc cgctatgtcg cggtagcggc gatcgcccgt gagttcgtag
     3061 taccgcgccg cggcgatcac ctgcgggata tgcgtgttga cgtgtaggcc tttcagttcg
     3121 tcgcggtgag cagccagcgg atcgaaaaac tgcttcttgt cgaagcgctg ggccacatgc
     3181 aggtagtact ccttgcccgt gaccgcgtat agattcgaca gcacttctcc catgccgccg
     3241 aattccgtgc ccagcactcg ctgcatgtgc tcgtaactga gtgaaccggt gtaaccggct
     3301 acccagcccg ccatcttctg caccatgtcg agcgcctgtt cgtcgccgca atagacgtac
     3361 atatccaaca ggccggccat gatcttgtga atggtgtaga acggagccca aacccgttca
     3421 cgctcgcgaa gacgatcgaa gaattcgatt ggaaacgcgc tcagatagcc gcttttcaga
     3481 gcagcctgac acctgccgag ttccgacacg acgaggctgg cgttgttttt gagatcttcg
     3541 tctcctgtgc tggcatacat cagcgcgcaa gccgaaaggt agtgtccgcc ggcgtagtgt
     3601 ccgcgaagtt cacaatcggg tgcttcccac ccacccaacg gttgggccga ggacggtaag
     3661 ccggcgttgc ttcgaaatgt gtgcagcaag cggtcgggcg ggagggagtg cagataccgg
     3721 cggtcggctt ccgtcgcgac ccgacaaggc ccgtctccca acttcacctg cttcatcggg
     3781 aacgggcgcg ccttccacgg aactttctcg tagctgacgg gctttccgtc tttgtgcgtc
     3841 gtatccacaa caggatcctg aacaggaact gtcttcaacg catctggaag tagtgcgaat
     3901 cccgctgaag ccaccacgga tctcatgaag ttcctgcgag agaagtccct gaattgcgcc
     3961 atagtaatcg ttctcccaga aatgtaggat gacccagaac cccgctccac atattgtata
     4021 cacccgcaga ttgcggtgca tttttggagg accgggtgaa ctaccgaatg gccgttgggc
     4081 gatgaccacc atccctccgt gcagcaacct acgatgacag aaatcctctg ctcgcgaatc
     4141 agtccggtag ctctgcaaca acctgagata atccgatcac actattgacg ctgccactcg
     4201 gactctcgat aatcggcgaa cttttggggt tcgactcgcc cccgacatgg cacacgtttc
     4261 gcggctgctg aactgtgctg cgcgcacatg tcgaggacga atcgtcaaaa cgactgtggg
     4321 gagccgcatg ccgcgtatcc gaagccttgt ccgggtgttg ctgacaatct tggcggcgtt
     4381 gacctgggtg acatgcaccg ccgcggccca gcaaatcacc ggcaacattc agggcacggt
     4441 caacgatccg agcagagccg tggtgcaatc tgccgtggtc acggtcgagc agatcgaaac
     4501 cggactttcg cgcagtgcgg ttaccgatcg tggcggccgc tacttgctgc tcgaactgcc
     4561 cgttggccac tatcgactgg aagtcgcagc gagaggcttc cggaagtatc tgcaggaagg
     4621 aatttccctg gacgtcaacg agaccgcaac ggtccatgtc catctgcaag ttggatccga
     4681 gacgcaacag atcagggtag atgccaatgc ggccctgatt caaaacaccg tcaccggcct
     4741 tgggcagacg gttatggagc acgagattct ggatctgccc ctggacgggc gagattttgc
     4801 ccagctcgga atccttcagc ctggagtcgt gccgctcact ccggggttga tccaggcggg
     4861 cggaggattg cgggacaacc agggctatgc cgtggatggg cagcggccgg agtccaacaa
     4921 tttcctcatc gacggagccg acaacgtaaa caacgtcgat gccgggtttg tgctgaaacc
     4981 gccgatcgat gcgatttcgg agttccggat catcacccac aatgccgacg ccgagttcgg
     5041 ccgaaacacc ggttccacta ccaacatcat cacccgctcc ggctccaact cctttcacgg
     5101 cgcggcatgg gagttcctgc gcaacgacgc catggacggt tcggactact tcacgcgaac
     5161 cgtgcaaccg ttaaagcaga atcagttcgg cgcgaccttc gggggcccaa ttgtccgcga
     5221 caagaccttc tttttcgcct actacgaagg attccgcaac cgccagggtg agacggattc
     5281 tgcgaccgtc ccgtcattgc ctgagcgtca aggaaatttc caggaactct gcaccaatcg
     5341 ccccctcaat ggacagttcg caggaggagt gtgcaccgat tccaagggca acttcctgcc
     5401 caggggccag ctgttcagcc ttttcaccgg tcagccaatc ccagtgccca acaacatgct
     5461 gaccggaccg tttgattcca ccgctcaaaa tgtcatgcct tttttccctc aacccaacag
     5521 tggtacgaac acattcatta ccacacaaac aaaacgtgag accaacgatc aagtcggcgt
     5581 tcggatcgat cactatctct cccggtcgga cagcctcaat ttccgttaca tgttcagcgg
     5641 cggcccgacc tttgacccgc tctcgccggc cggtgcgaac gtgccagggt ttccaattgg
     5701 cgcggacgac cgggcccaga atttcgtcgc gcaggagagc cacacatttt cagcgagtgt
     5761 catcgccgtc gcacgcttct cgtatctgcg taataaattt ctctttggcg accagatcaa
     5821 tcacgaatca cccacacagc tgggttttca gtatgagccc agcctggggt ctgccgctgg
     5881 cccgccgttc atccaggtcg gcggctacgc ctccgttggt gatcccatta ccgggccgcg
     5941 aaacactttc cagaatacct tcgactactc cggttcgctg agctgggttc acggcgctca
     6001 cgatctgaag tttggcggtg gctatcgtca cgacggcatc aacgtgctgc aaggcatcgc
     6061 caccaatggc tttttcgttt tcgctccttt cccgtactcc gacgggtttg ccagcttcct
     6121 ctccggcgtt cctgtgtttt ttctgcaggg cgtaggaaat ttctcgcgcg gtattcgcgg
     6181 ccaggcgtcg aacgtctacg cccaggacac cttcaaggtg actccacggc tgacgatgaa
     6241 ctacggtttg cgctacgaac ttccctcgcc tgacaccgaa attcacaacc gtcagaatct
     6301 cttcattccg ggcgctcagt ccaaagtgtt tccgggtgct ccggcaggct tgttgtatcc
     6361 cggagatccc ggcgtgccgg ccggcttgat ttccaccgac aggaagggtc ttgctcctcg
     6421 catgggcgtt gcctgggatc ccactggcag ctccagatgg ctgctgagag cggcatacgg
     6481 tattttctac gagccgtact acaccggcca gggcgggcca ctgcaggccc cgattagtgc
     6541 tccaccctac attcaaacgc cgcaggttaa cggcccgcag tcgtttgcga atccgtacct
     6601 gaaccagagt ttgttcaacg gtccgtttgc cgagccgatg actcttctga ctttggctcc
     6661 caaactttct ctgccctacg cgcaggactg gaacctgacc atccagaaat cgttcggcga
     6721 caactggctc gcagaagtgg gatacgtagg gacgaacggg agcaagctgc cgcgattcat
     6781 cgagggaaat cccactacct tcgtcccggg agtcgactcg agaggaaacc cgatttcgaa
     6841 cgaaaacaac gtcaaccagc ggcgccttta ctccggatgc actctggcgc agcccaatgg
     6901 ctgcgtttat gcctcggccg gcctgatcgc gggagtcgct aactcgtctt acaacgccct
     6961 cgaagccagt ttgcggaagc gtttcagcca tggcttgtcc tttctggcct cctatacgct
     7021 gtcgaaatcc attgatgatg tttcgtcctt taacatcacc ggttccgcat cgcagccggt
     7081 tgccggagag aacgatctgg cgcaaaatcc tttcgatctc tccgccgagc gcggacgctc
     7141 catgttcgac gcccgccacc gtttcgtgct gagctatcag tggaaccttc ctttttggaa
     7201 tcgtccctcg ggctggtacg agcatgtgct ggggaactgg caattgaacg gtattgcaac
     7261 tctgatgagc gggacaccct tcaccgtgtt cgattcctcc gacaactctc tgcaaggagg
     7321 cgcaccggaa atcaccggct tttccgcgaa tcgcccgaac ctgattgcaa acccgaattc
     7381 cggacctcgc actccgcagg agtggatgaa cgtcaatgct ttccagcaac tagcccccga
     7441 tcccctcgga cgattccagg tattcgggaa cgaagggcgg aatgtggtcg agggaccggc
     7501 gtacctgaat tgggatttct cggctttcaa gaacatcccc atatcggagt caagagaatt
     7561 ccaatttcgc ggggaattgt tcaacttcct gaaccacacc aatttccgat tgcccgttag
     7621 cgatatcagt tcgccaaact tcggccagat tcagcaagat gttgggccgc gcgtcattca
     7681 ggtggcgttg aaatttctgt tctagggcgg caccaatgcc gcggctccga atccggatct
     7741 cgtccggcgt gtgagcgtcg accttgcgaa cgtggacggc ggcttctgct tcggcgctag
     7801 atcggtcgga ccatcccctg agctgtcgcg cgcgtgttgc gcaccagcag cagcaccagg
     7861 atcatgcctg caaacggaac cagcccggcg gtaattacga tcgggtcaaa ggaatgcgtt
     7921 gcttgcgcct ggcgtgcatc cgacaggtag ccgatgagtt tgaacgcgac gatggttccg
     7981 ataccggcgc cggttcccga cattccgctg accgacgcga ccgaatcgct caggaaaaga
     8041 tcggcgggca gcacatttgc gatggtagag aaggatgcgt aagaaaaagt agacagagcg
     8101 aacagaaggg tgatggccca cacattggcc gtgagaaccg tcggaatcag cagcagcacg
     8161 ccgcaggcgc cgaacacgac cagcgccttt cgcgctgcgc caaccgtcca gccgcgcttg
     8221 atgaggtagc ctgacgcgcc gccgccaaag aaatttccca ggtcggccgc gatgaacgga
     8281 acccataccg cgatcaatcc gctctgcaga gagatgcctt tggcaaccag ataaatagga
     8341 aaccagtcgg tgacgaaaaa ccagaccggg tccgtgagag tcttggcgaa gattgcgccc
     8401 catgtctgcg gcaggcgcaa cagatctctc cagcgcggtt tggacccttt cgctgtgatc
     8461 tcggggtcgt cgcggtcctg cagaatcatc tccagttcct cgggggagat acggcggtgc
     8521 tcttccggag ggcgatacga acgccgccac acgtacagcc acaggaagcc gagcagtccc
     8581 ggaatcacga atgcaggacg ccatccccag tggaagtaaa ccttcagcac gatgaaagga
     8641 gcgatcgctc cgccgatgga agatccgctg tcgaacaacg cggttgccaa ccctcgctcg
     8701 cgtttcggaa accattcgga cacagccttc gtcgcgcccg gccagttggc ggattcgccc
     8761 gcacccagca gaaagcgaaa gccggcaaag ctgtagaacc cgcgcgccag ggacgtagca
     8821 actgccacca gcgagtacca cgcgacggat aggctcaacc cgcgtcgtgt gccgacgcga
     8881 tccattccgc ggccacacaa tgtctgtccg atcgagtatg cgacccggaa accgatcacg
     8941 atgttcgcgt agtcggtatt ggtccagtgg taggtctgtt tcaggtaggg agccagattg
     9001 gcaagcgttt ggcgatcaat gtagttgatc acggttgacg cgaacagcag ggcgccaatc
     9061 caccagcgca agtgccgaat gggaggtctg gccggcgacg agtcttgtgg aatggtcccc
     9121 atagctcacc ccgcagcatc ggcaggtcag tcgcgccgtg ccgcgaatca gcggttctgg
     9181 cggtaatggt ctatctctga gcctgcacct gctggcgaga gttcaacaac gccgccgact
     9241 gcaggtcaag aaagaccgtc acattgggat gcatccgtag tgccgaagcc ggggccaggg
     9301 ggctgatgtc gccctcaaga caggctttca ccgccttggc ctttcgcgcc tccggaactg
     9361 gtgcgatgat ctgtcgcgcc ttgagtatct ggcgg
//
LOCUS       DASPUL010000640         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_395864, whole genome shotgun sequence.
ACCESSION   DASPUL010000640 DASPUL010000000
VERSION     DASPUL010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395864"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1131)
                     /locus_tag="VEJ00_14765"
     CDS             complement(<1..1131)
                     /locus_tag="VEJ00_14765"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXX02476.1"
                     /translation="MEPFRFHLFVCTQQKPEGVPSCPASGSFAVLDALDHAIQERKLD
                     QDLQLTTCGCMGLCDEGPMMVVYPEGVWYRRVKPADVPEIVDTHLRAGKPVDRLAWND
                     SAAMKAMAVEHTEKFRAAMAAREKAGTLPDRLDQMIRGYMPSRCLLTALELDIFTAVG
                     AGANAERIGAKINANPRAAGMLLNALVSLGLLSKTGDEYRNTPESARFFTQGSKDNHR
                     NGLLHIANIWHRWSTLTDAVRVGTRIPIDRDRTPEWTRSFIAAMQGNAGERAPLVVKA
                     LGTAGVRRILDLGGGSGAYSIAFAKASRDTQCEILDLPEVVPLTADYVDKAGVAAQVK
                     LHPGDMLHDNFGSGYDLVMLNAICHMFSEEQNQDIFRRARQAL"
     gene            1347..1922
                     /locus_tag="VEJ00_14770"
     CDS             1347..1922
                     /locus_tag="VEJ00_14770"
                     /inference="COORDINATES: protein motif:HMM:NF014852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HXX02477.1"
                     /translation="MRKLNVILYISVLCALVLPGRAMPDQTSRPEQELKLVPAPQEVE
                     LRGPGGFRVGARTKILVQLGHQEEDRIAAETLAETVSDQSGLQLDILGMKAAGKAEGG
                     AIVLARLQDARVRRFLARKGLRADELVGEQGYLLFSDKSHLIVAANSGQGLFSGVQTL
                     RQLLRPAGKELLCPAVTIRDWPRLTESAMKN"
     gene            2091..>2495
                     /locus_tag="VEJ00_14775"
     CDS             2091..>2495
                     /locus_tag="VEJ00_14775"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXX02478.1"
                     /translation="MFSDRTNWRLAQNRLTLALQEARARGSKILDLTVSNPTRVGLEY
                     DSHAILAALGSARALDYDPQAKGLPAAREAVAAYYRRDHGVHDLDAERIVLTTSTSEG
                     YSFVFRLLCNAGDELLVPKPSYPLFEFLADLQD"
BASE COUNT          483 a          668 c          819 g          525 t
ORIGIN      
        1 gagagcctgg cgggcgcggc ggaagatgtc ctgattttgc tcctcggaaa acatgtggca
       61 gatcgcgttc agcatgacaa ggtcgtagcc agagccgaag ttgtcatgga gcatgtcgcc
      121 ggggtgaagc ttaacttgcg ctgcgacccc tgctttatcg acgtagtcag cggtaagcgg
      181 cacaacctcc gggaggtcaa ggatctcgca ctgcgtatcc cgcgaggctt ttgcaaaggc
      241 gatagagtag gcacccgagc cgccgcccag atcaagaatg cggcgaacac ccgcggtacc
      301 gagggccttt accacaagcg gggcgcgttc cccggcgttt ccttgcatgg cagcaatgaa
      361 gctgcgtgtc cactcaggcg tgcggtcgcg atcaatcgga atgcgcgtgc cgacacgtac
      421 ggcgtcggtc agggtgctcc agcgatgcca gatgttggcg atgtgcagca ggccgttgcg
      481 atggttgtct ttggacccct gggtgaagaa gcgggctgac tcgggcgtgt tcctgtactc
      541 gtcgccagtt tttgagagca gtccgaggga gaccagagca ttgagcaaca tgccagccgc
      601 acgcgggttc gcgttgatct ttgctccgat ccgctcggcg ttggctccag cacctacggc
      661 ggtgaagatg tcgagttcga gcgcagtgag aagacaacgg ctgggcatgt agccgcgaat
      721 catctgatcg aggcggtccg gcaatgtgcc ggctttctcg cgcgcagcca tcgccgcgcg
      781 gaacttttca gtgtgctcga cggccatggc tttcatcgcc gccgaatcgt tccacgccaa
      841 gcgatccacc ggcttgcctg cgcgcaggtg cgtgtcgacg atttcaggca catcggccgg
      901 cttcacgcgc cggtaccaca ccccttccgg atagacgacc atcatggggc cttcgtcgca
      961 cagccccatg cagccgcagg tggtaagctg caaatcctgg tcgagtttgc gttcctgtat
     1021 ggcatgatcg agcgcgtcga gcacagcgaa tgatccgctg gctgggcaag agggtacgcc
     1081 ttctggtttt tgctgtgtgc aaacgaaaag atgaaagcgg aatggttcca tgagaggtct
     1141 ccggtcgata tgagttgtgt agaattgctt ctcggatcag gtctacacct caatgttagc
     1201 gctttgcggg gcggggaggg cgaacagcgg cggtagataa accatgattt tggttagagt
     1261 tatcgggatg ttgacaaagt tgagacacag atccctccac acaccggtac aatagtgggg
     1321 ttcgttggaa ggtttttcag aactgaatga gaaaactcaa cgttattctg tacatctccg
     1381 tcttgtgtgc gctggttctt ccgggccggg cgatgccgga ccagacgtcg cggcctgagc
     1441 aagaactgaa gctagtgccc gcgccgcagg aggtagagct gcgcggcccc ggcggttttc
     1501 gcgtgggggc gcggacaaag atccttgtgc agctgggcca ccaggaagaa gatcgcatcg
     1561 cagccgagac tctggccgaa acggtctccg atcagtcggg cctgcagctt gacattctgg
     1621 gcatgaaggc cgcggggaag gcggaaggcg gcgcgattgt gttggcacgg ctacaagacg
     1681 ctcgcgtgcg gcggttcctg gcaaggaagg gtctgcgcgc cgatgaactc gtgggtgaac
     1741 aggggtatct tttgttctcg gacaaatcgc acctgattgt ggccgccaac agcgggcagg
     1801 gattgttttc cggtgtgcag accctgcggc agttgctgcg tccggcgggg aaggagcttc
     1861 tctgtccagc ggtaacgatt cgggactggc cccggcttac cgaaagcgca atgaagaatt
     1921 gaacagcggc gtagaaggaa gagatcagcc gtccttttgg gacggcttgt ttgttggagc
     1981 gtgagacgcg ttgggcgcga agtcagggtc gaggcccttc acgctcttat tctttcgttc
     2041 ctgcctgtca gaagtacccg ccatcccata gaatcccgga tatcgaaagt atgttttctg
     2101 atcgcaccaa ctggaggctg gctcagaacc ggctgaccct ggcattacag gaggcgcgcg
     2161 cccggggctc gaagattctc gacctgacgg tatcgaatcc gacacgggtg gggttggagt
     2221 atgactcgca cgcgatcctg gcggcactcg gttcagcacg cgctctggat tacgatccgc
     2281 aggcgaaagg attaccggcg gcgcgggagg cggtggcggc ctactatcgg cgggaccatg
     2341 gtgttcatga tctcgatgcg gagcgcatcg tgctgaccac cagcacgagc gagggatatt
     2401 cgttcgtctt ccggttgctt tgcaatgcgg gcgatgagtt gctggtgccg aagccaagct
     2461 atcccctgtt tgagtttttg gcagatttgc aggat
//
LOCUS       DASPUL010000641         3740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_804386, whole genome shotgun sequence.
ACCESSION   DASPUL010000641 DASPUL010000000
VERSION     DASPUL010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804386"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..338)
                     /locus_tag="VEJ00_14780"
     CDS             complement(144..338)
                     /locus_tag="VEJ00_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02479.1"
                     /translation="MNFANWPEWLKLLVLVPHGILASVACWLWWPKSEKGWRKFGFVV
                     AYLIVFYLVMRFVFQMKDFK"
     gene            complement(475..1701)
                     /gene="asnA"
                     /locus_tag="VEJ00_14785"
     CDS             complement(475..1701)
                     /gene="asnA"
                     /locus_tag="VEJ00_14785"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HXX02480.1"
                     /translation="MSLFISEDRSGTLADSYEQVTVENGSAPALDSQASKKADLAGPG
                     IGDYEELEKILPRDYHPLLTPRETQTAIFHVKRYIEDNLCKELKLMMVEVPLIVDVES
                     GVNDMLDRDGSRTPIQFHISNDRDKHPIDAQVVQAATKWKRVALKQFECRSGEGICTD
                     MRAVRKDYFLDHDHSAYVDQWDWERAITAEQRNLEFLKSIVRKIWKVLVGAEKFAQEM
                     FPELRQPRHPNLPEELTFIHAEELLAMYPDLPRKRRETVILQKHPAVFIIGIGWPLKD
                     GYPHEMRAADYDDWITDTSAESRRDTHGLNGDILVWNHVTQRRHELSSMGIRVNAETL
                     RQQLEMSKLLDNLRFPYHQAIINNEIPLSIGGGIGQSRTLMLLLRKAHLGEVSVTVWP
                     KILKEMCAKKNIVVLE"
     gene            2126..2485
                     /locus_tag="VEJ00_14790"
     CDS             2126..2485
                     /locus_tag="VEJ00_14790"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXX02481.1"
                     /translation="MAADTGKDPMSEEQVNALQYEDLQSAPRFPLHLTASVKGRDGAY
                     SAETENISANGVLFAMDRDVPVGSMVDFTIMLPADVVGARQDVQIDCRGRVVRSFDDR
                     GRRGVGVVIDEYHFERA"
     gene            2500..3357
                     /locus_tag="VEJ00_14795"
     CDS             2500..3357
                     /locus_tag="VEJ00_14795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXX02482.1"
                     /translation="MASIPVNVEENLGAGGDEKGQFVRVIVADTQAIFRAGLRKIFAL
                     EDDIRVVGQAETLAQTQTAVKKFSSDVLIFEAALTPNPVEAVAELLRQNPQLRVVVVT
                     PTADEEMTLDLFRRGAHGIVSREVEPELLVDCLRKVAAGETWLEDKAVHWVMEAYRTQ
                     GNRPAGARPKVQLTPKETLIVSCVTQGMKNKEIALRVGTTEQVVKNYLRKVYDKLGVA
                     DRLELALYCLSHHVVDSTKVPPLPASPANGHAAAAAAGASTSPASGNSAPANGADASE
                     PVVALPKPI"
     gene            3516..3605
                     /locus_tag="VEJ00_14800"
     tRNA            3516..3605
                     /locus_tag="VEJ00_14800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3550..3552,aa:Ser,seq:gga)
BASE COUNT          772 a         1092 c         1077 g          799 t
ORIGIN      
        1 acaggtcact ccatatcagc cttctgttgc tttgggtatt tttctatgcc gccatcgggc
       61 agttcttcta agacggatgg ttgacgttac atcgcgattt cactcactgt cgcaagactg
      121 acggtcttga gcgttcgcgt cgattacttg aagtccttca tctgaaagac gaagcgcatg
      181 accaaataga aaacgatcag gtacgcgacc acaaatccga acttgcgcca ccctttttct
      241 gacttcggcc accataacca acaagcgacc gatgcgagaa tgccgtgcgg cacaagaacg
      301 aggagtttca accattcggg ccagttggcg aagttcatca ccgagcaatt ctaacgtggc
      361 tcttcgtcaa ctgacgtaat atcgcgatta cactctctga ccctgattgg aacacattgt
      421 gcaacggaaa cgggaccgct atgccgtccc gttatcaccc ccagctaagc aagcttactc
      481 cagcacaaca atattcttct tcgcgcacat ctcctttagg atcttcggcc atacggtaac
      541 gctgacttcg cccaagtgcg ctttgcgcag caggagcatt agcgtgcggg actggccgat
      601 gccgccgccg atcgagagcg gaatctcatt gttgatgatg gcctggtgat aagggaacct
      661 gagattgtcg agcaactttg acatctcgag ttgctgacga agagtctcgg cgttgacccg
      721 gatgcccatc gaacttagtt catgccggcg ctgggtgacg tggttccaga ccaggatgtc
      781 gccgttcaag ccgtgcgtgt cgcggcgact ttccgcgctc gtgtcggtga tccagtcgtc
      841 atagtcggcg gcgcgcatct cgtgcggata gccgtctttc aacggccagc cgatgccgat
      901 gatgaaaacg gccggatgct tctgcaaaat gaccgtctcg cgccgtttgc gcggcaggtc
      961 gggatacatg gcgagcagtt cttcggcatg aatgaaagtc agctcctcgg gcaggttcgg
     1021 atgtcgcggc tggcgcagtt cggggaacat ctcctgcgcg aacttctcgg ctccgaccag
     1081 aaccttccag atcttgcgca cgatggactt cagaaactcc aggttgcgct gctcagccgt
     1141 gatcgcccgc tcccagtccc attgatcgac gtaggcagag tggtcatgat cgaggaagta
     1201 gtctttgcgt accgcgcgca tatccgtgca gatgccttct ccggagcggc attcgaactg
     1261 cttcagtgcg actcgtttcc atttcgtcgc cgcctgcacg acctgcgcgt cgatgggatg
     1321 cttgtcgcga tcgttcgaga tgtggaactg gataggagtg cgggagccat cgcgatcgag
     1381 catgtcgttg acgccgcttt ccacgtcgac aatgagcggt acctcgacca tcatcagttt
     1441 cagttccttg cagaggttgt cctcgatgta gcgtttcacg tggaagattg cggtctgtgt
     1501 ttctctggga gtcagcagcg ggtgatagtc gcggggcagg attttttcga gttcctcgta
     1561 gtcaccgatt cctggtccgg ccaaatcggc tttcttgctg gcttgggagt cgagcgcggg
     1621 cgcgctcccg ttttcaaccg taacctgctc atagctgtcg gccagagtcc cgctgcggtc
     1681 ctcggagata aacagactca taatgattgc tccttcggca gcacaacaga tagctgcgac
     1741 attcctaccg gtattcaagt tccccattca acggcgaaca gtgatgccag tcacacagga
     1801 aggtgctgcg agtaacagcc agacgacccc ctccgcgtca tcccagatgg ccagaggtga
     1861 ttgatttttc ctctcgtcgc aacgccggaa cttagccgaa attcaccctg attcaaccga
     1921 aaatgcaggg gtctgagagg cagtttccgg cctcctgtgc ggctccatga attgccgcta
     1981 atccattcaa agaaaagcag ttaactccca acgttccatg acttcggtaa ctttgcacgg
     2041 ctgattcccg ggtgtacgct gcccttgagt tccgcgagtg tgaaagacag gcacaacgct
     2101 cccggaagaa aggttgtagc gcgacgtggc agccgacaca ggcaaagatc cgatgtccga
     2161 agagcaagtc aacgcgctgc agtacgaaga tctgcagagt gccccgcgct ttccgctgca
     2221 tttgacggcg tcagtgaaag ggcgagacgg cgcctattcg gcggaaacgg agaacatctc
     2281 cgccaacggc gtgctgttcg ccatggaccg cgatgtgccc gtgggttcca tggtggattt
     2341 cacaatcatg ctgccagccg atgttgtggg ggcgcgtcag gatgtgcaga tcgattgccg
     2401 ggggcgcgtt gtccgcagct ttgatgaccg cggccgccgc ggcgtgggcg tggttattga
     2461 cgaataccat ttcgagcgcg cctaggcgca aaggactgaa tggctagcat tccggttaac
     2521 gtggaagaaa atctcggagc cggcggcgac gagaagggcc agttcgtgcg cgtgatcgtt
     2581 gccgataccc aggccatctt ccgcgccggt ctgcgcaaga tcttcgcgct cgaagacgac
     2641 attcgcgtcg tgggccaggc ggaaaccctg gcgcaaaccc agacggcagt caagaaattc
     2701 tcttccgacg tattgatctt tgaagcggcg ctgacgccga atccggtcga agcggtggct
     2761 gaactcctgc ggcagaatcc gcaactgcgt gttgtcgttg tgactcccac agcggatgaa
     2821 gaaatgacgc tcgacctgtt ccgccgcggc gcccatggca tcgtctcgcg cgaggtcgag
     2881 cccgaactcc tcgtggactg cctgcggaaa gtggccgcgg gagagacctg gctggaagac
     2941 aaggccgtgc actgggtcat ggaagcgtac cgcacccagg gcaaccgtcc ggcgggcgcg
     3001 cgtccgaaag tgcagctcac gccgaaggag acgctgattg tttcctgcgt gactcagggg
     3061 atgaagaaca aagagatcgc gcttcgcgtc ggcaccacgg agcaggtggt gaagaactac
     3121 ctgcgcaagg tgtacgacaa gctgggtgtc gcggatcgtc tcgagcttgc cctgtactgc
     3181 ctgagccatc acgtggtcga cagcaccaaa gttccgccgc tgccagcctc gccggcaaac
     3241 ggccatgcgg ccgccgccgc tgccggagct tccacctcac cggcgtcggg aaattccgca
     3301 cccgccaacg gcgccgacgc ttccgaaccc gtcgtcgcgc tcccaaagcc catctgatcc
     3361 tccccgcagg catgctcgct taccggttgc ggtttctgca acgtcaccca tagaatcaaa
     3421 aagaccccag gagccagctg cgatcggggg ttggtcaggc aggctccagg ccgaacgacc
     3481 ttgcagtaga gtgatccctt ctcagatttc gcgccggaga gatggccgag tggctgaagg
     3541 cgcacgcttg gaaagcgtgt ttactcgaaa gggtaacgtg ggttcgaatc ccactctctc
     3601 cgccagaatt tcagtaactt agcgtgctcc cctgccaaac tcacctgcca tttacagcta
     3661 acgctgccac tcttgaagca gctacagcca tttcgttggt gacaacatcc ccgtagacat
     3721 tcagggttgt cctgatgtcg
//
LOCUS       DASPUL010000642         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1624568, whole genome shotgun sequence.
ACCESSION   DASPUL010000642 DASPUL010000000
VERSION     DASPUL010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624568"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1081)
                     /gene="thiD"
                     /locus_tag="VEJ00_14805"
     CDS             complement(209..1081)
                     /gene="thiD"
                     /locus_tag="VEJ00_14805"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HXX02483.1"
                     /translation="MAEKPPVVLTIAGFDPSSGAGVTADIKTIAAHGCYGVACISAMT
                     VQSTSGVRRVEPADPALVTDTLEELAADVDIDAVHLGMLGSGKVVKAVADFLAPSSPE
                     RSGTRGKARFPNIVLDPILKSSSGAALLDATGTKLLVDRLIPLADVVTPNVDEAEAIT
                     GIKVREIEEMKAAAAMLHEMGAPAVVITGGHLEKAIDLLSFTTKRGIEQEIFKAERQR
                     SNSTHGTGCAFATAMACHLALDRGIAEAALLAKTYVAAAISYGHPLGRGTGPVHHLYR
                     MSQQRRAAGSGSES"
     gene            1531..2040
                     /locus_tag="VEJ00_14810"
     CDS             1531..2040
                     /locus_tag="VEJ00_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXX02484.1"
                     /translation="MRRRLAHGLALFVLIASLGAAQGPNISEATPAPVSSVQIKPEIR
                     KQAGKTKPYQIGTASWYGEYFVGKPTASGEPYDMYDMTAASLTIPLGTYVRVTNLRNG
                     RAVVVRVNDRGPVVPGRIIDLSYEAAEALQFRERGLQRVRLDIVKPGPQRAHYQTVAL
                     NTPPLAELP"
     gene            2069..>2488
                     /gene="pyrF"
                     /locus_tag="VEJ00_14815"
     CDS             2069..>2488
                     /gene="pyrF"
                     /locus_tag="VEJ00_14815"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HXX02485.1"
                     /translation="MSPSRVAKSVSDSGNDPRQRLIIALDVSTAAAAQKIVAAVGDSA
                     LTYKVGMQLYTAEGPQVVRDLVASGRRVFLDLKYHDIPNTVGAAVSEAAKLNVSMLTV
                     HAAGGGKMLRAALEAATARPGLMVLAVTVLTSLDANDL"
BASE COUNT          511 a          743 c          735 g          499 t
ORIGIN      
        1 gtcgattggc aagccatcca gttctaccgg gcggaatggc tgctgggcgc gacggcatcg
       61 tttgtagccg gaatcctgct gaagaagccc tcaggaaact gactcccgct caagcctgct
      121 gttgttgcgt aaaccacgtc gtgagtcaag cctcggagtt ggacgcactc cactcttcta
      181 ctctgctctg cttctgtttt tcgagcgcct acgactcgct gcccgagccg gcggctcggc
      241 gttgctggct catgcggtag agatgatgta caggcccggt tccccgtccg aggggatggc
      301 cataggaaat ggcggccgca acgtaggttt tcgccagcag cgcagcctcg gcaatgccgc
      361 ggtcgagcgc cagatgacag gccatcgccg tagcgaaggc acagccggtg ccatgggtcg
      421 aattcgatcg ctgccgctcg gccttgaaga tctcttgctc gatcccacgc ttggtggtga
      481 agctcagcag atcaatcgcc ttttcaagat gccctccggt gattaccaca gcaggagctc
      541 ccatctcgtg aagcatggcg gcggccgcct tcatttcctc gatttctctg accttgatgc
      601 cggtgattgc ctcagcctcg tccacgttgg gcgtcacaac atcggctaga gggatgagtc
      661 gatccaccag cagcttcgtt ccggttgcat caagcaaagc ggctccggac gaggacttga
      721 ggataggatc aagcactatg ttcgggaacc gggccttccc gcgggttccc gagcgctcgg
      781 gggacgacgg ggccaggaaa tctgccaccg ccttcactac ttttcctgat cccaacatgc
      841 cgaggtgaac cgcgtcgatg tcaacgtcgg ccgcgagctc ttcgagggta tcggtcacaa
      901 gagcgggatc cgcaggttcc actcgccgta cacctgaggt ggactgtacc gtcatggccg
      961 agatacaggc cacgccgtaa catccgtggg cggcgatggt cttgatatcg gccgtgaccc
     1021 cggcccccga cgaaggatcg aagccggcaa tggtaaggac gacgggcggt ttttcggcca
     1081 tggacttcgg ttccgggcgg atacgatagt acccaacttc aggctcagca tctcacaggt
     1141 cgagagcccg ggaagttact ttagggccct ggcagcagga aaaaatgttc tcatgtgcct
     1201 tacggcccca cctcgcaacc tgtttattat gcgtaactta cactaactac ctccgtaagt
     1261 cgctgattac aatgggattg taaattctac ccggcctgcc gagggctgtt atttaccata
     1321 tgataaatac ataaccctac tgaaatgaat gggctttatt gtatccttat aaattcccaa
     1381 tgggttacgt aagatattga ttactacgag gtcatcactg accccccgaa ggggccttga
     1441 cactcgtttg gccccccgct agcattccac aatcgagtcc aaaaaccaaa gcctaggcta
     1501 ggacactttt gagtatgcga ggagaactga atgcgaagac gtttagctca tggactggcg
     1561 cttttcgtac tgatcgccag cctgggagcg gctcagggac cgaacatctc ggaggcgaca
     1621 ccagctccag ttagttccgt tcagatcaag cccgaaatcc gcaaacaggc cggcaagacc
     1681 aagccgtatc agatcggcac ggcttcgtgg tacggagaat acttcgtcgg gaaaccgacc
     1741 gccagcggcg agccttatga catgtacgac atgaccgccg ccagcctgac gattccgctt
     1801 ggaacctatg tgcgggtgac caacttgcgc aatgggcggg cggtagtggt ccgggtcaac
     1861 gaccgcgggc cggtcgtacc gggccgcatc atcgacctct cgtacgaggc agccgaggcg
     1921 ctgcaattcc gcgagcgcgg cctgcagcgc gtgcggctgg acatagtgaa gcccggcccg
     1981 cagcgagctc actaccagac cgttgccctc aacactcctc cgctggcaga actcccctaa
     2041 aaaacataca ctggcaggga tgccagcgat gtcaccgagc cgggtcgcga aaagcgtgag
     2101 cgattccggg aatgatccgc gccagcggct cattattgcc ctcgacgtct cgaccgccgc
     2161 cgcggcgcag aagatcgtgg cggcggtggg cgattccgcg ctcacctaca aagtcgggat
     2221 gcagctctac acggccgaag ggccgcaggt ggtgcgggat ctggtcgctt cgggacggcg
     2281 cgtgtttctc gacttgaagt atcacgacat cccgaacacc gtgggggcag ccgtttccga
     2341 agcggccaag ctgaatgtca gcatgctgac cgttcacgcc gcaggtggtg gcaaaatgct
     2401 gcgcgctgcg ctggaagcgg caacggcgcg tcccgggctg atggtgctcg cggtcacggt
     2461 gctgaccagt ctggacgcga atgatctt
//
LOCUS       DASPUL010000643         4915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1770079, whole genome shotgun sequence.
ACCESSION   DASPUL010000643 DASPUL010000000
VERSION     DASPUL010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770079"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="VEJ00_14820"
     CDS             complement(<1..514)
                     /locus_tag="VEJ00_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02486.1"
                     /translation="MSRKVNRRDILKGLAAASAVGMIPPAWNEDDASQRPVELQVTAV
                     SAHSFRLSILPVNNGETGTIPANGSLVQESWGSPETFRTAPKGEFAVAGCRLTISLNP
                     VRIKIANERGNSIQQLDWDANSGQLSFLSGNQPLFGMGEGGPQFDRRGSVDRMKSGQG
                     GYKLATHGGRV"
     gene            697..1926
                     /locus_tag="VEJ00_14825"
     CDS             697..1926
                     /locus_tag="VEJ00_14825"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXX02487.1"
                     /translation="MRWRIAILISAAIAISYLDRQTLPVAVKAISKDIPLSNSQFSAL
                     QSAFLFSYALMYAGGGRLVDFLGTRRAFTAIMIFWSLACASHALATGFAMLAASRFLL
                     GVGEGGGFPAATRAVSEWFPPAERATAMGIINAGTAVGAVIAPPLIAAVLGYANWRWI
                     FVLTGGLGLLWAARWRFSYVPPRASVTEEVEPGTATTWSWTHLLATRETWGLVFAKFL
                     SDAAWFFYLFWLPKYLYDARGFDIKAVGTFAWMPSAAAGIGCLLGGGFSSFLMRRNFS
                     LGASRKLALGLSAVIMPLVILVPHVAVPWVITLFCLGYFGQQSWSTLVMVLPADLFPA
                     EVVGSVAGLVGFGGAMGGILFGELVGYLLDHNFGYSVVFNIAGTLHVAAFLIILAWVP
                     VLLPPAERRRVELGMVS"
     gene            1923..3095
                     /locus_tag="VEJ00_14830"
     CDS             1923..3095
                     /locus_tag="VEJ00_14830"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HXX02488.1"
                     /translation="MKITEIRTRVVRWRGKTVPLPPHFCTNPMDLLELPETSMQTFTF
                     HGWLLVEIFTDNGLVGLGNAALAPQVTKQVIDLYLKPLLIGQDPWDSERIWQHMYRKT
                     MAFGRKGIGMVAISAVDIALWDLLGKSAKQPVYRLLGGRTKARIPVYASRLYSVELSE
                     LAAEAKRYKNEGYRAMKFRFGWGPADGASGMQRNLELVRTVREAVGDEIDVMADAYMG
                     WTLDYARRMLPLLEPFHLRWLEEPVIPDDVHGYAELKSCGLIPIAGGEHEFTLYGFRD
                     LLEARALDYIQFDTNRVGGITQARKIAALAEAYSVPVIPHAGQMHNYHVVMASLNSPM
                     AEYFPIVDVEVGNELFWYIFNGEPRAKEGFIDLDDNLPGLGLSTNEDGLKQFDVME"
     gene            3118..4119
                     /gene="araD1"
                     /locus_tag="VEJ00_14835"
     CDS             3118..4119
                     /gene="araD1"
                     /locus_tag="VEJ00_14835"
                     /inference="COORDINATES: protein motif:HMM:NF040903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraD1 family protein"
                     /protein_id="HXX02489.1"
                     /translation="MTRLVQLKKDNMRRVALVDEPNLRLLDNCSSIYELAQKAIAAGL
                     KLSDVARQRARHDALDYDSIYSGRSDWRLLPAVDHPHEPSRCLISGTGLTHLGSARGR
                     QSMHAVAPEDLTDSMKMFRWGVEGGRPAEGSIGTPPEWFYKGMGCTLRAHGEPLEIPS
                     YAEDGGEEAEVAGIYLVGQDGQPYRIGMATGNEFSDHQFEKKNYLNLAGSKLRTCSIG
                     PELVADPEFESVAVSATIEREGKVHWTKTFRSGEREMCHSLQNIEHHHFKFEAHRRPG
                     DVHVHFFGTDCLSYSDQIRLQDGDVMEVAVDGYGRPLRNPLRICKSEAVLIRAIPLG"
     gene            4128..>4915
                     /locus_tag="VEJ00_14840"
     CDS             4128..>4915
                     /locus_tag="VEJ00_14840"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXX02490.1"
                     /translation="MSKASVAILGLGIMGSGMANRLLGAGFPLTVYNRTPAKSKPFAD
                     AGAHIASTPREAASRAKIVLSMVGDDAASREIWLGSDGALGGAAPNSILIESSTLTGA
                     WIHALAAKAAERGCRFLDAPVTGTKPHAASGELVFLVGGAAEAVEAARPVFSVLGSDT
                     IHLGPVGSGALMKLINNFVCGVQAASFAEGMSLIDAGGLDRGKAVSILTSGAPGSGIV
                     KRVAERTAAGDSTPNFALRWMAKDLSYALGEASARKIALQTAAAA"
BASE COUNT          904 a         1487 c         1505 g         1019 t
ORIGIN      
        1 gcacacgtcc accgtgggtg gcgagcttgt agccgccttg tccgctcttc atgcggtcga
       61 cggacccgcg gcggtcgaat tgcggtccac cttcgcccat gccgaacaat ggctggtttc
      121 cgctcaggaa cgagagctgg cccgagttcg cgtcccaatc gagttgttga atagagttgc
      181 ctcgctcgtt tgcgattttg atgcggactg ggttaagaga aatggtcagc cggcaaccag
      241 ccacagcgaa ctcgcccttg ggcgcggttc gaaaggtctc aggggatccc cacgattcct
      301 gcaccagcga tccgttggca ggaatcgttc ctgtttctcc gttgttcact ggcaggatgc
      361 tgaggcgaaa actgtgagcg ctgaccgcag tcacttgcag ctcgacggga cgctgggaag
      421 cgtcgtcctc attccaggcc ggcgggatca tcccgactgc ggaggccgct gccaggcctt
      481 tcagaatgtc gcgacggttt acctttcgac tcataggtgc gctcgcactg gaacggaagt
      541 gtgtggcgac cgctggctgg ctcacgctga ccgaggctcc gggtcaaagc gagaagtcga
      601 gcgattgtag ccgcaacagg gctgtggagt aaacgtttac cattttcgcg tataatgccc
      661 acgcgttccc gatccccctt ggcacggagg cagcacttgc gttggcgcat cgccatcctg
      721 atcagcgctg cgatcgcgat cagctatctc gaccgccaga ctttgcccgt cgccgtgaaa
      781 gcgatcagta aggacatccc gctgagcaac agccagtttt ccgccctgca gtctgcgttc
      841 ctgttttcct acgccttgat gtacgccggc ggcggcaggc tcgtcgattt tctcggcacg
      901 cgccgcgcct tcaccgcaat catgattttc tggtcgctgg cctgcgccag ccatgctctg
      961 gcgacgggtt tcgcgatgct ggcggccagc cggttcctgc tcggcgttgg cgagggcggt
     1021 ggcttccccg ctgcaacccg cgcagtgtcg gaatggttcc ctcctgccga acgggcgacg
     1081 gccatgggca tcatcaacgc cggcactgca gtcggcgcgg tgatcgcccc gccgctcatc
     1141 gcggcggttc ttggctacgc caactggcgt tggatcttcg ttctcaccgg cgggctgggg
     1201 ttgttgtggg cggcgcggtg gaggttctcg tacgtgcctc cccgtgcgtc tgtcaccgaa
     1261 gaagttgagc cgggcaccgc aacaacctgg tcgtggacgc acctgcttgc cacgcgcgag
     1321 acctgggggc tcgtgttcgc gaaattcctg agcgatgctg cctggttttt ctacctcttc
     1381 tggcttccaa aataccttta cgacgctcgc ggttttgaca tcaaggcagt cggaactttt
     1441 gcctggatgc ccagcgcagc agcgggcatt gggtgtcttc tcggcggcgg attctccagc
     1501 ttcctgatgc gacgcaactt ctctctgggt gcatcccgga agctcgcgct cgggctgagc
     1561 gctgtcatca tgcccctggt aattctcgtc cctcatgttg cggttccctg ggtgatcacg
     1621 ctgttctgcc ttgggtattt cggacagcag tcctggtcga ctctggtgat ggtgcttccg
     1681 gccgatctgt ttcccgccga agttgtcggc tcggtggcgg gcctcgtcgg cttcggggga
     1741 gccatgggcg ggattctctt tggggagctg gtcgggtatt tgctcgatca caacttcgga
     1801 tactcagtgg ttttcaacat cgccggcacc ctgcatgtcg cggcctttct gatcattctt
     1861 gcctgggttc cagtcctgct gccgccggcg gagcggcgaa gagttgagct ggggatggtt
     1921 tcatgaagat caccgagatt cgcacgcggg tggttcgctg gcgcggaaaa accgttcccc
     1981 tcccgcctca cttctgcacc aacccgatgg atcttctgga attgccagaa acctccatgc
     2041 agacgttcac ttttcatggc tggctactgg tggaaatatt cacagacaac gggcttgttg
     2101 gattgggcaa tgccgccctg gcaccgcaag taacgaaaca agtgattgat ctttacctga
     2161 aacctctgct catcggccag gatccgtggg acagcgaacg tatctggcag cacatgtatc
     2221 gcaagacgat ggcgttcgga cgcaaaggca tcggcatggt ggcgatcagc gcggtcgaca
     2281 tcgcgctctg ggaccttctc ggtaagtcgg ccaagcaacc cgtttaccgc ctgctcggag
     2341 gccgaacgaa agcccgcatc ccggtctacg cctctcggct ctacagcgtc gaactgagcg
     2401 agctggcggc cgaagccaag cgctacaaga acgaaggcta ccgcgcgatg aagttccgct
     2461 tcggctgggg accagcagac ggggccagcg gcatgcagcg aaatctcgaa ctcgtgcgca
     2521 cggttcggga agccgtcggc gacgaaatcg acgtgatggc cgacgcttac atgggatgga
     2581 cactcgacta cgccaggcgc atgctaccgc tgctcgagcc ctttcatctg cggtggctgg
     2641 aagagcccgt gatcccggac gacgtccatg gctatgccga actcaaatcc tgcggactta
     2701 tccccatcgc gggaggcgag cacgaattca ctctttacgg cttccgcgac ctgctggagg
     2761 cgcgcgcgct cgactacatc cagttcgata ccaaccgcgt cggcggcatc acacaggccc
     2821 gcaagatcgc cgcactcgcg gaagcgtact ccgtgcccgt catcccgcac gccggccaga
     2881 tgcataacta ccacgtcgtc atggccagtc tgaattcgcc gatggcggag tacttcccga
     2941 ttgtcgacgt ggaagtgggc aacgaattgt tctggtacat cttcaatggc gaacccaggg
     3001 caaaggaggg tttcatcgat ctcgacgaca acctgcccgg tctcgggctg agcactaatg
     3061 aagacggcct caagcagttc gatgtgatgg agtagtcctg gaaatcaaaa cgcttcgatg
     3121 acacgactcg ttcaactcaa gaaagacaac atgcggcggg tggctctcgt cgacgaaccc
     3181 aacctgcgat tgctcgacaa ttgctcgtcg atctatgaac tggctcaaaa ggcgattgcc
     3241 gcgggcttga agctcagcga cgtggcgcgg caacgcgccc gccatgatgc tcttgactat
     3301 gattccattt acagtggccg ctccgattgg cgcctgcttc ctgccgtcga tcatccgcac
     3361 gagccgtctc gttgtctgat ctccgggact gggctgactc atctcggcag tgctcgcggc
     3421 cggcaatcga tgcatgccgt tgcgcccgag gatctcaccg acagcatgaa aatgttccgc
     3481 tggggcgtcg agggaggccg cccggccgaa ggcagtatcg ggactccgcc ggagtggttc
     3541 tacaagggca tgggctgcac cctccgtgct cacggcgagc cgcttgagat tccatcttac
     3601 gcagaggatg gcggcgagga ggcggaggtt gccggaatct acctcgttgg ccaggatgga
     3661 caaccgtatc gcatcggcat ggctacggga aacgaattct cggatcatca gtttgaaaag
     3721 aagaactacc tcaacctcgc cgggtcgaag ctgcgcacct gctccatcgg gccggagctt
     3781 gtggcggacc ctgagttcga atcggtcgcg gtgtcggcga cgatcgagcg agagggcaag
     3841 gtccattgga caaaaacatt ccgcagcggg gaacgcgaaa tgtgccacag cctgcaaaac
     3901 atcgagcatc atcacttcaa attcgaagct caccgccgcc cgggagacgt tcacgtccac
     3961 ttcttcggca cggactgttt gagctacagc gatcagattc gtttgcaaga cggagacgtc
     4021 atggaagtgg ccgttgacgg ttacgggcgt cctttgcgaa acccgctgcg catatgcaaa
     4081 tcggaagcgg tcctgatcag ggcaattcct ctgggatgag tgaacctatg tcaaaagcca
     4141 gcgttgcaat cctcggactc ggcattatgg gctcgggaat ggccaatcgt ctgctgggag
     4201 cgggtttccc attgaccgtt tacaaccgca ctccggcaaa atccaaaccc ttcgccgacg
     4261 ccggagcgca catcgccagc acgccccgcg aggcagcctc cagagcgaag atcgtcctaa
     4321 gcatggtcgg ggatgacgcc gcttcgcgcg aaatctggtt gggtagcgac ggcgcactgg
     4381 gtggcgctgc tccgaattcc atcctgatcg aatcgagcac tttgaccggt gcgtggattc
     4441 acgcgctggc cgccaaggcc gccgagcgcg gctgccgctt cctggatgcg cctgtgacag
     4501 gaacgaagcc tcacgccgcg tcgggggaac tcgtgtttct tgtcgggggc gccgccgaag
     4561 ccgtcgaagc cgcgcggcct gtattctccg tgctcgggag cgacaccatc catctgggcc
     4621 cggtgggcag cggcgctttg atgaaactca tcaacaactt tgtctgcgga gtgcaggccg
     4681 cttcctttgc ggagggcatg tccctgatag atgcgggcgg cctcgatcgc ggcaaggctg
     4741 tgtcgatcct gactagcggc gctcccggaa gtggaatcgt gaagcgagtg gcagagcgaa
     4801 cggcggccgg cgattccact ccaaactttg ccttgcgctg gatggcgaaa gacctctcgt
     4861 atgcgttggg cgaggcctca gccagaaaga ttgctctgca gaccgccgcc gctgc
//
LOCUS       DASPUL010000644         3317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2046332, whole genome shotgun sequence.
ACCESSION   DASPUL010000644 DASPUL010000000
VERSION     DASPUL010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2046332"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="VEJ00_14845"
     CDS             <1..1995
                     /locus_tag="VEJ00_14845"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HXX02491.1"
                     /translation="LAVAQSQPQLNLMPMPANIQLGTGQSLITQSFAVSSTGYRNAVG
                     ADILRFQEQLSRQTGIPFRLKPGVAPVFEIHSEHGRQAVQKLGEDESYELTITDSHAK
                     LTSQTWLGALRGMQTFLQLVQITPAGFAVPAVTIKDQPRFPWRGLLIDVSRHFIPLDV
                     LKRNLDGMAAVKMNVLHWHLSDDQGFRVESKRFPKLTEMGSEGHYYTQEEIREFVAYA
                     HDRGIRVVPEFDMPGHSRSWFLGYPELSSGSDPYTLEGGGVDPTMDPTREQTYKFLEK
                     FVAEMARLFPDAYFHIGGDEVDGKQWDANPKIQAFKQAHGMKNNQDLQAHFNQRLQKI
                     VAKNHKIMVGWDEILHPDLPKSIVVQSWRGQASLAIAAQQGYSGLLSHGYYLDLMWPA
                     ARHYAVDPMSDAAANLSPEEKSRILGGEACMWAEWVTPENIDSHIWPRNAAIAERLWS
                     PQEVTDVSSMYSRLNAVSLDLEWLGLTHRSARTHMLQRMAGDADISALQMLADVVEPV
                     KDYNRWDDAKQGPIDFHAPLNRMIDAAYPESDLARHFTDLVHSFVLSGYRDKAAEGLL
                     RNQLSSWRDNDAGLRPLLDQSFLLEEDAAVSQNLSALGAAGLQALEFLDKSQTAPDSW
                     KVSQLALIEQVKKPQAGLVLMVVAPVQQLIEAAGGQIPPN"
     gene            2240..3046
                     /locus_tag="VEJ00_14850"
     CDS             2240..3046
                     /locus_tag="VEJ00_14850"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX02492.1"
                     /translation="MSAEAYSFSDLLEIKKVRDLCAMSVRPTVIRQSLDAMRKQAAGV
                     EHPLLEAGTWSTAKHRVAFRHEGKLLEPIAGQFLMDFSEREKVVTTTPVPRTEPTPRE
                     NEVAAWFAQGISLEEDPNTQTEAMAAYHKVIEVEPAHAAAHINLGTLYYNRQDFALAE
                     SHYRQAIEADPRYALAYFDLGNVLDETGRVQEATQTYKTALQLAPTYADAHYNLALAY
                     EKMRQPRKALKHWQAYVKLDTSGPWSVHARSQIKRILQAEGLKLVYKRSS"
     gene            complement(3094..>3317)
                     /locus_tag="VEJ00_14855"
     CDS             complement(3094..>3317)
                     /locus_tag="VEJ00_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02493.1"
                     /translation="GRPMKLDFVPVAALEEQHRSSDPLQKTFAALMLGYAKGDVIEDA
                     TTLANQYGIMLRSVSEYARRVRQQMQSVA"
BASE COUNT          690 a         1009 c          965 g          653 t
ORIGIN      
        1 ctggcagtag cccaatccca gcctcagctg aatctcatgc ccatgcccgc caatattcag
       61 cttggcacag gccagtcact catcacgcag tcgttcgcgg tgtcgtccac cggttaccga
      121 aatgctgttg gagccgacat cctacggttt caggaacagc tatcccgcca aactgggatc
      181 ccctttcgtc tcaagccggg cgtggcaccg gtgttcgaga ttcactccga gcatggccga
      241 caagctgtac agaaactcgg tgaggacgaa tcttatgaac tcacaatcac cgactctcat
      301 gccaagttga cgtctcagac atggctcggc gcacttcgcg gcatgcagac atttttgcaa
      361 ctagtgcaaa tcacgccggc aggctttgcc gtcccagccg tcaccatcaa ggatcaaccg
      421 cgtttcccgt ggcgtggcct gctcattgac gtcagccggc acttcatccc gctcgatgta
      481 ctcaagcgca atctcgacgg catggccgcc gtgaagatga acgtcctgca ctggcatctc
      541 tccgacgatc agggattccg cgtcgagagc aagagattcc ccaagctgac agagatggga
      601 tcggagggcc actactacac gcaggaggag attcgcgagt tcgtcgccta cgcgcacgat
      661 cgcggcatcc gcgtggtccc cgagttcgat atgccgggtc atagccgctc ttggtttctg
      721 ggttatcccg agctttccag cggctccgac ccttacacgc tcgaaggcgg tggcgttgat
      781 ccgactatgg atcccactcg cgagcagacc tacaagtttc tcgagaaatt cgttgccgag
      841 atggctaggc tgttccccga cgcttacttt cacatcggtg gcgacgaagt cgacggcaag
      901 cagtgggatg cgaatcccaa aattcaggcc ttcaagcagg cgcacggaat gaagaacaat
      961 caggatctgc aggcccactt caatcagcgc ctgcagaaga ttgtggccaa aaaccacaaa
     1021 atcatggtcg ggtgggacga gatcctgcat cccgatttgc ccaagagcat tgtggtgcaa
     1081 tcgtggcgtg ggcaggcctc gctcgcgatc gccgcccagc agggctacag cgggctgctc
     1141 tcgcacggct attaccttga cctgatgtgg cccgcggcgc ggcactatgc ggtcgatccg
     1201 atgagtgatg ctgcggcaaa tctgagcccg gaagagaaga gccgcattct cggcggcgag
     1261 gcttgcatgt gggcagaatg ggtcacgccg gagaacatcg actcgcacat ctggccacgg
     1321 aacgcagcta ttgccgagcg cctttggtca ccgcaggaag ttaccgacgt cagctcgatg
     1381 tattcgcgct tgaacgccgt gagcttggac ctggaatggc tgggactgac ccatcggtcc
     1441 gcgcgtacgc acatgctgca gcgcatggcg ggagacgcgg atatctcggc gctgcagatg
     1501 ctcgccgacg tcgtcgagcc ggtgaaggac tacaaccgct gggatgatgc gaagcagggc
     1561 ccgatcgact ttcacgctcc gctgaaccgc atgatcgacg ctgcctatcc ggaaagtgac
     1621 cttgcccgac acttcaccga cctggttcac tccttcgtcc tgagcggata cagagacaaa
     1681 gctgcagaag gtctgctacg aaaccagttg agcagctggc gagacaacga cgctgggctc
     1741 cgtccgctcc tggatcaatc gtttctgctg gaggaagacg ctgcggtgtc ccagaatctg
     1801 tctgcgctcg gcgcagcggg tttgcaggct ctggagttcc tggacaaatc gcagaccgcg
     1861 ccggattcgt ggaaggtatc gcaactggcc ctgatcgagc aggtgaagaa gccgcaagct
     1921 ggccttgtac tgatggtggt agcgccggtt cagcagttga tcgaagccgc tggtgggcag
     1981 ataccgccga actaagtcga cgcaatgttc acgtttgcac catctccgct gcatgtaaat
     2041 caaccaggtt ctccacagct tttcacaccc cttagcagtt gcgtttgtgg cgaggcttca
     2101 cgataataac cgcgaagatg cttgtgggct tgcggtagga ctcccggtta cacgtactgt
     2161 gtacagtcga gctgacttac tgaggattct ccatatcacg ccgcgccagt tggcgaactg
     2221 ggagcgcctt ggtctgacca tgtccgcgga agcttattcc ttttccgact tgctggaaat
     2281 caaaaaggtt cgcgatctgt gcgccatgag tgtgcggccg acggtgatcc gccagtcgct
     2341 cgacgccatg cgcaaacagg ctgccggcgt cgaacatccg ttgctcgaag ccgggacctg
     2401 gtcgacggcg aaacaccggg tcgcattccg ccatgaaggc aagctgctgg agccgatcgc
     2461 cggccagttc ctgatggatt tctcggaacg cgagaaggtg gtcaccacca cgccggtgcc
     2521 gcggacagag cctactcccc gggagaatga ggtggcggcc tggtttgccc agggcatctc
     2581 gctggaagag gatcccaaca cgcagaccga ggcgatggcc gcctaccaca aggtgatcga
     2641 agtcgaaccg gcccacgccg cggcccacat caatctgggg acgctctatt acaaccgtca
     2701 ggattttgcg ctcgctgaga gtcactaccg ccaggcgatc gaggcggacc cgcgctacgc
     2761 tttggcctat ttcgacctcg ggaatgtcct ggacgaaacc gggcgcgtgc aggaagcgac
     2821 tcagacgtac aagaccgcgc tgcagctcgc tcccacctat gccgacgctc actacaatct
     2881 cgcgctcgcc tacgagaaaa tgcgccagcc gcgtaaagcg ctgaagcatt ggcaggccta
     2941 cgtgaagctc gatacttcag ggccgtggtc ggtgcacgcc cgcagccaga tcaagcgcat
     3001 cctgcaggcc gaggggctga aactggtcta caaacgcagc agctgacatg acatcggctg
     3061 tcttgttcct tccgtgctcc gacgattccg ttcctaagcc acgctctgca tctgctgacg
     3121 tactctccgc gcatattcgg agaccgaacg aagcatgatg ccgtactggt tggccagagt
     3181 cgtggcatcc tcgatcacgt ctccctttgc gtaaccgagc atcagcgcgg cgaaggtctt
     3241 ctgcagggga tcggatgagc ggtgctgctc ctcgagtgcc gccaccggaa cgaaatccag
     3301 cttcatcggc ctgccaa
//
LOCUS       DASPUL010000645         4660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1221217, whole genome shotgun sequence.
ACCESSION   DASPUL010000645 DASPUL010000000
VERSION     DASPUL010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1221217"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VEJ00_14860"
     CDS             <1..296
                     /locus_tag="VEJ00_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02494.1"
                     /translation="MVFGAWCDRQYGFVYDAIRAHHGLAWFPVLDVERNIHWEPNPFY
                     RSAGLSIQNPRAYPELGLDPGTPIYQHFATDPESVQWVSEPARVAPLWADFEM"
     gene            complement(309..1640)
                     /locus_tag="VEJ00_14865"
     CDS             complement(309..1640)
                     /locus_tag="VEJ00_14865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HXX02495.1"
                     /translation="MGINRYLVKSTFVSALGGLLFGFDTAVIAGTTHQLTEVYHLTPS
                     LLGLTVSSALWGTVLGAMTAGYPGQRFGRRDSLRIMAVFYFLSALGCALAWNWGSLLA
                     FRIIGGLGIGGSSVLAPMYIAELSPAKWRGRLVGCFQVSIVVGILLAYLSNFLIGRMN
                     FGLAEWRWQLGIAALPAFFFLIMLFFIPRSPRWLALKNRTQEALAVLQLTGNENPERE
                     LDAIVASIHLERSAAGEPLLQRKYLLPIVLAVTAGAFNQLTGINACLYYLNDIFAAAG
                     ASKYSAGMQSVTIGCTNLIFTLIAMSVIDQFGRKKLLLTGTAGVCVFLAAIGQIFRSG
                     IHQGWLIWLVMAFMGFFAISQGAVIWVYISEVFPTRVRAKGQAIGTSTLWVMNALISG
                     IFPILAKKSSATPFFFFASMMLIDFILVAAVYPETKGVSLEELEQRLGVAN"
     gene            1769..3997
                     /locus_tag="VEJ00_14870"
     CDS             1769..3997
                     /locus_tag="VEJ00_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02496.1"
                     /translation="MGSGRDLDRRTFLKLGAAGTASVAINPPARASLVVPKGALSSPP
                     SLADLASDRLTYRLRDLFNCPVAMNELGYAQTGKSVSAITAISFPPYACCGVPETTWS
                     PGFLLTCEVFLNGRYLAIAASPDGNVEYQWFPHCVVRTQTAERLRFTTRMFLPSKRRA
                     VAQSIAVKNLSGARRLFTLGFDMRGAVAKKAKPWFVNSPGEVENRVTWDAHRGCLLFE
                     AQHSEIAAAEGFHPVPSRVDQMRMLHFDLELGAGEQKELNFVLAIAENGRAALGLYDG
                     LQAGFADVEKENELALASLVEAAFTPNNSEFSGNLPRLVTRDESLWKLYHNGFANLLF
                     ARRVSPDSAYGPTYLTLSGHVLPTLSFPWDTSLTSLALALLDPIPLRNLVEVWLKAGM
                     HEHHSTDYVTGEPVGPWYAANDTAIVRCAWRYLCVTGDFEWLDKSIADRTVLDHLDEH
                     ATYWKKLDHFERGLGDYGTIENLLEVVSTYLHEVAGMNAGNVHSMRIVADLQERRGNS
                     TRGRELRGEATDLAERINRLLYVDGKGYWRCGQPDGTFNEVRHCYDFLAVLDNMAEDL
                     TPKQRREMSQFFWRELHSEKWMRALAQSDADATWNIRPDHSCLGAYAAWPPLTAKGLY
                     KIDSPEKIVPWLREVARAGNQGPIGQAHFVEDVYPPVNGGAFKSSEDAPFIEDWCCIA
                     AGAFTELVIDSIFGAELTLSDGIKVNSRLASFDPTARLEGLRYQGSLYTVTKDGAQKQ
                     KA"
     gene            complement(4003..>4660)
                     /locus_tag="VEJ00_14875"
     CDS             complement(4003..>4660)
                     /locus_tag="VEJ00_14875"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HXX02497.1"
                     /translation="DALEQKGLLQRTHGGAVSIEPLFYEPFRRDRSFLAQVERAAEEK
                     RRIGRAAAALITAGETIALTPGTTTTEVIRGLPINYNITVVTNTVNVAMELSKRKDVY
                     VFVTGGHLHGEWFSLVGSAALRSLENMLINTMFIGADGMDATWGASCFNADEAELNST
                     MMKLARRRVAVVDSGKFGVVANWRICKADELNILVTDTAATDEMVAPFQKLGIEVMRV
                     "
BASE COUNT         1019 a         1359 c         1346 g          936 t
ORIGIN      
        1 gcatggtgtt cggtgcctgg tgcgaccggc agtatggatt cgtgtatgac gcgatccggg
       61 cgcaccacgg cctcgcctgg tttcctgtgc tagacgttga acggaacatt cactgggagc
      121 cgaatccttt ctatcgatcc gctggcctct cgattcaaaa cccgcgtgcc tatccggagc
      181 tgggattaga tcccggcacg ccgatatatc agcactttgc aaccgatccc gagagcgtcc
      241 aatgggtttc ggaaccggcg cgagttgcgc cgctatgggc ggacttcgaa atgtaagtct
      301 cttctggatt agttggcgac gcccagccgc tgctccagct cttccagcga gactccctta
      361 gtctcggggt acaccgcggc gaccaggatg aagtcgatca gcatcatgct cgcaaagaag
      421 aagaagggag ttgcactcga tttcttcgcg aggatgggaa aaatacctga tatcaaggca
      481 ttcatcaccc agagtgtgga tgtcccaatc gcctgacctt tggcgcgaac ccgcgtggga
      541 aagacttcgc tgatatacac ccaaatcact gcgccttgcg agatggcgaa gaaacccatg
      601 aaggccatca ccagccagat cagccatccc tggtggatgc cgctccggaa gatctgcccg
      661 atcgcggcca ggaaaacaca tacgcccgcg gttccagtga gcaaaagctt tttgcggccg
      721 aactggtcga tgacgctcat ggcaatcagg gtgaaaatca gattggtgca tccgatggtt
      781 acggattgca tgccggcgga atacttgctg gctccggccg ccgcaaaaat gtcattcagg
      841 tagtacaggc acgcgttgat tccggtcagc tggttgaagg cgccggcagt cacagccagt
      901 acgatcggca gcaggtattt ccgctggaga agcggctcac ctgcggcact acgttccaga
      961 tgaatcgaag ccacgatcgc atccagctcg cgctcgggat tttcgttacc ggtcagctgt
     1021 agaacagcga gagcctcctg cgtgcgattc tttagcgcca gccaccgcgg gctgcgtgga
     1081 atgaaaaaca acattatgag gaagaaaaat gcggggagag cagcgatacc cagctgccaa
     1141 cgccattccg ccaagccaaa gttcattcgg ccaattaaga aattcgacaa gtacgccagc
     1201 agaatgccca caacaatgct gacctggaag caaccaacca ggcggccgcg ccacttcgca
     1261 ggtgacagct cagcgatata catgggcgcg agcaccgacg atccaccaat tcctaaccct
     1321 ccaatgatcc ggaaggcgag cagcgatccc cagttccagg ccaaggcgca gcccagcgca
     1381 gacaaaaaat aaaatacggc cataatccgc agactgtctc gccggccaaa ccgctgtccc
     1441 ggatatcccg ccgtcatggc ccctaggacg gtgccccaga gagcgctcga gaccgtcagc
     1501 cccagtaagg aaggagtaag gtggtagacc tcggtcaatt ggtgggtggt gcctgcgatc
     1561 acggcagtgt cgaagccaaa cagcagtccg ccaagagcgc tcacaaacgt gctcttcacc
     1621 aagtaccggt ttatgcccat gagttctttt ccttcggacg caatgaacga atactagcga
     1681 aaagaacata attggtctat gaaatagcgt gtttagacgg tgtaaacttc cgatagttca
     1741 agagaaggtc ttagcgcccg aggagtcgat gggctctggc agggatctcg atcgtcgcac
     1801 attcttaaag ttgggagcgg ccgggactgc ctcggttgcg attaaccctc ccgcgcgtgc
     1861 gtctttggtg gtccccaaag gggcgctcag ttctccccca agcctggccg acctggcatc
     1921 ggatcgactg acctaccggt tgcgcgatct attcaactgt cccgtcgcga tgaacgaact
     1981 tggctacgca caaacaggaa aatcggtttc ggcaatcacc gcaatctcgt tcccgcccta
     2041 cgcctgctgc ggagtacccg aaaccacgtg gagccccggc tttctcttga cctgcgaagt
     2101 cttcctgaat ggccgctatc tcgcgattgc cgcctcgccc gacggcaatg tcgagtatca
     2161 gtggttcccg cattgtgtgg ttcgcacgca aaccgccgag cgactgcggt ttacgacccg
     2221 catgttcctg ccaagcaagc gccgtgcggt ggctcaatcg atcgcagtta agaatctgag
     2281 cggggcgcga cgtctcttta cgctcggctt cgacatgcgc ggagccgtcg ccaagaaagc
     2341 caaaccctgg tttgtgaatt ctcctggcga agtcgagaac cgggttacct gggacgctca
     2401 ccgcggttgt ctgctgtttg aggcacagca ttccgagatc gccgctgccg agggatttca
     2461 tccagtaccc agtcgagtgg atcagatgag gatgctgcac ttcgacctag aacttggagc
     2521 aggcgaacaa aaggaactca acttcgtact cgcaatcgcc gaaaatggcc gggctgcctt
     2581 gggactctac gatggcctgc aggcagggtt tgccgatgtc gagaaagaaa acgagttggc
     2641 tcttgcgagc ctcgttgaag ctgcgtttac tccgaacaat tccgaattca gcggtaacct
     2701 gccgcggctt gtgaccagag atgaatcgct gtggaagctc taccataacg gctttgccaa
     2761 tctgcttttc gcccgccggg tctccccgga ctcggcgtac ggcccaacgt atctaacctt
     2821 gagtggacat gtgctcccga cgctgagctt tccgtgggat acgtcactca cctctctggc
     2881 gcttgcgctg ctcgatccga ttccgctgcg caaccttgtg gaagtgtggc tgaaggccgg
     2941 aatgcacgag catcattcca cggattatgt gacgggcgag cccgtgggtc cgtggtatgc
     3001 agcgaacgac accgcgatcg tccgctgtgc ctggcggtat ctatgtgtga ccggagattt
     3061 tgagtggctc gacaagagta ttgccgatcg caccgtcttg gatcacctcg atgagcacgc
     3121 cacctactgg aaaaagctcg accacttcga acgcggtctc ggcgactacg gaacgatcga
     3181 gaatctgcta gaggtcgtca gcacctatct gcacgaagtg gccggaatga atgccggcaa
     3241 cgtgcacagt atgcggatcg tcgccgatct gcaggaacgc cgcgggaact cgactcgcgg
     3301 cagggaactg cgtggcgagg caacagacct ggcggaacgg atcaaccgtt tgctctatgt
     3361 agatgggaag ggatattggc gttgtggcca gcctgacgga accttcaacg aggttcggca
     3421 ctgctatgac tttcttgccg ttctcgacaa tatggctgag gacctcacgc cgaagcagag
     3481 gcgcgaaatg agccagttct tctggcgcga actgcacagc gagaaatgga tgagggccct
     3541 cgcgcagagc gacgccgatg caacctggaa tatccgtccg gaccatagtt gcctgggcgc
     3601 atatgcggcg tggccgccgc tgacggcaaa gggactatac aaaatcgatt cgccggagaa
     3661 gatcgtgccc tggctgcggg aagtggccag ggccggcaat caaggtccga ttgggcaagc
     3721 gcacttcgtg gaagacgtct atccgccggt caacggcggg gcgttcaagt catcggaaga
     3781 tgcgcccttc atcgaagact ggtgctgcat agcggctggt gccttcacgg agctcgtgat
     3841 cgactcgatt ttcggcgccg agctcacgct ctcggatgga attaaggtca actcccgcct
     3901 agccagcttc gatcccacgg ctcgactgga gggtctgcga taccagggca gtttgtacac
     3961 ggtcacgaaa gacggggcgc agaagcagaa agcctgattt gctcagactc gcatgacctc
     4021 gatgccgagc ttctgaaagg gcgcgaccat ttcgtcggtc gccgctgtgt cggtgaccag
     4081 gatattcaac tcatccgctt tgcagatccg ccagttcgct actacgccga atttgccgct
     4141 atccaccact gcgacacggc gccgcgccag cttcatcatc gtcgaattaa gttccgcctc
     4201 atccgcattg aagcagctgg cgccccaggt ggcatccatg ccgtctgcgc cgatgaacat
     4261 cgtgttaatc agcatgttct cgagcgaccg caaggcagca gagccgacta gcgagaacca
     4321 ttcgccatgc aggtgtcctc cggtcacgaa aacatagaca tccttgcgct tggagagctc
     4381 catcgccacg ttgacggtgt tcgtgaccac cgtgatgttg tagttgatgg gcagcccgcg
     4441 gattacctcg gtggtcgtgg tgcccggagt caatgcgatt gtctcgcctg cggtgatcag
     4501 cgcggcggcg gcccggccaa tgcggcgttt ctcctcggcg gcacgctcta cttgagccag
     4561 gaaggatcga tcgcgacgga acggctcgta aaaaaggggc tcgatcgaca cagccccgcc
     4621 atgggtgcgc tgcagcaatc ccttctgctc cagagcatcc
//
LOCUS       DASPUL010000646         2697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1079181, whole genome shotgun sequence.
ACCESSION   DASPUL010000646 DASPUL010000000
VERSION     DASPUL010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079181"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VEJ00_14880"
     CDS             <1..275
                     /locus_tag="VEJ00_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02498.1"
                     /translation="LLARFVHALRQRRVAADQRVSAFALWAFLFYILFMIGSFVEAPF
                     EFPSGAVPFYFLIGFSLGMIRWHMSPKNKSERIIEFPMTRERAYLQ"
     gene            320..1087
                     /locus_tag="VEJ00_14885"
     CDS             320..1087
                     /locus_tag="VEJ00_14885"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXX02499.1"
                     /translation="MEQPRIVTTSWDDGDRADLRLAEMLRSRGIAGTFYVPTKPYGTR
                     PALTHADLRALSSEGFEIGAHGVTHKLLWGLSEQELTEEIGPCKPALEDIVGKEVRMF
                     CYPCGRYDANAVRSLKNAGYSGARTVRMLATGFEFNPFEIPTTVQIIPQPRTSYFKNI
                     ARSRRLDSFRTGLVNLTRLGNWLELGKKLFDSVLEKGGIWHLYGHSHEIEKLDLWEDL
                     GRILDYVGKREGVKYVPNCDLIGLQVAHGGHAGNGKL"
     gene            1084..>2697
                     /locus_tag="VEJ00_14890"
     CDS             1084..>2697
                     /locus_tag="VEJ00_14890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXX02500.1"
                     /translation="MKIILVHCHYQQPGGEDVVFEQERKLLERAGHQVVVFRRSNFEA
                     DLYPGVKRLVLLKKAVWSGDSQDAFARLLRREKPDLVHVHNTWVMISPSIYSACREAG
                     VPVVQTLHNYRLLCPVGTFFRDGKICEECVDHSLIRSFQNGCYRDSRAETAAVALMLT
                     VHRNRHTWDREVDSFIVLTEFARRKFLRGGLPEEKVFVKPNFVDPDPQPRDGDGEYAI
                     FAGRLSPERRVSTLLDAWTHLRSRIPLMIVGGGGQRDLLEMNAARENLGMVKFTGLLA
                     HDKTIAAIKKARFLIFASEWYETFGLTMIEAFACGVPVICSRMGAMQELVDDGRTGLH
                     FNPGDARDLAEKVEWAWNHPERMREMGEEARREYEQKYTAESNYPRLMEIYRHAMATQ
                     AAESLQPDRKASAAAAADATPAPASQGPQRAALSVNTGSDDASPVNGSHKNGHLRIVV
                     AHNYYQIPGGEDETLRRERDLLRSAGHSVKEFIRRNSEIAPADIVAKMSLAARTVWAG
                     DSRKAMLQFLREENPHVAHFHNTFPLMSPS"
BASE COUNT          568 a          783 c          817 g          529 t
ORIGIN      
        1 gcctattggc gcgtttcgtt cacgccctcc gacaacggcg tgtcgcggca gaccagcgag
       61 tatcggcctt cgccttgtgg gcgtttctgt tctacatact gtttatgatc ggcagctttg
      121 tcgaggcccc attcgagttc ccctcaggcg cggtcccgtt ctacttcctg attggatttt
      181 ctctgggaat gattcgttgg cacatgtccc cgaaaaataa gagcgaacgg attatcgaat
      241 tcccgatgac ccgggaacga gcttatttgc agtaggtctc acaacgaaat aacaggcgat
      301 ttcggaaatg ccacaaggta tggaacagcc aagaatcgta accacgagct gggacgacgg
      361 tgaccgcgcc gatctgagac tcgcagagat gctgcgttcc agaggaatcg cgggtacgtt
      421 ctatgttcca accaagccct acgggactcg tcccgccctg actcacgcgg atctgagagc
      481 actgtcatct gagggttttg aaatcggcgc tcacggagtt acgcacaaac ttctgtgggg
      541 attgtcggag caggaattga ccgaggaaat cggcccctgc aagccggcgc tggaagatat
      601 cgtggggaag gaagtccgga tgttctgcta cccctgcggc cgctacgacg cgaatgcggt
      661 gcgcagtttg aaaaacgccg ggtattcggg agcccgcacg gtacgcatgc tggccaccgg
      721 tttcgaattc aaccccttcg agattccgac cacagtgcag attatccccc aacctcgaac
      781 cagctatttc aagaacattg cgagatcgcg gaggctggac agctttcgca ccggtctcgt
      841 gaacctcacc aggctgggca actggctcga actcggcaag aaactcttcg actcggtact
      901 cgaaaaaggc ggcatctggc acctgtatgg ccattcgcat gaaattgaaa agctcgacct
      961 ttgggaagat ctgggaagaa tcctcgacta tgtgggcaaa cgggaaggcg tgaagtacgt
     1021 ccccaactgt gacctgatcg gcctgcaagt cgcacatggc ggccacgccg gaaacggcaa
     1081 attgtgaaga taatcctcgt ccattgtcat tatcagcaac cgggcggcga agacgtcgtc
     1141 tttgaacagg agcgcaagct cctggagcgc gccggacacc aggttgtggt tttccggcgg
     1201 agcaatttcg aggccgacct ctatcccggc gtcaagcggc tggtgttgct gaaaaaggcg
     1261 gtgtggagcg gcgattcgca agatgctttc gccaggctgc tgcgcaggga gaagccggat
     1321 ctggtccacg ttcataacac ctgggtcatg atctcacctt cgatctactc ggcgtgccgc
     1381 gaggccggcg tgccggtggt gcagaccctc cacaactaca ggctcttgtg tcccgtgggg
     1441 acgttcttcc gcgacggcaa gatctgcgag gaatgtgtcg accacagcct gattcgcagt
     1501 tttcaaaacg gctgctatcg cgactctcgg gctgagacgg ccgctgtcgc cttgatgctg
     1561 acggtgcatc gcaatcggca tacgtgggat cgggaagtcg attcttttat cgtgctgacg
     1621 gagtttgcgc gcaggaagtt tctccgcggc gggttgccgg aagagaaagt attcgtcaaa
     1681 ccgaacttcg tggacccgga tccgcagccc cgggatgggg atggagagta cgccatcttt
     1741 gccggccggc tctcacccga gcggcgcgtc agcacgctgc tggatgcctg gacccacctg
     1801 cgcagccgca ttcctctgat gattgtcggc ggaggcgggc agcgcgatct gttggaaatg
     1861 aacgccgcgc gggaaaacct cggcatggtc aagttcacgg gcctgctggc gcacgacaaa
     1921 actatcgccg cgatcaagaa agcgcgcttc ctgatctttg ccagcgagtg gtacgagact
     1981 tttggcctca ccatgatcga ggcgttcgcc tgcggcgtcc cggtgatctg ctcgaggatg
     2041 ggcgcgatgc aggaactggt cgatgatggc cgcaccggct tgcatttcaa tcccggcgat
     2101 gcccgcgacc tggcggagaa ggtcgagtgg gcgtggaatc atcccgagcg catgcgggag
     2161 atgggcgagg aagcccgccg cgagtacgaa caaaagtaca ccgccgagag caactatccc
     2221 cgcctgatgg agatttaccg gcacgccatg gcaacacagg cggccgaatc gctgcagcct
     2281 gaccgcaagg caagtgccgc agcagccgcc gacgccaccc cggcgccggc gtcacaaggg
     2341 ccccaacgcg cggcccttag cgtaaacaca ggctcagacg acgcgtcgcc ggtgaacgga
     2401 tctcataaga acggacatct gcggatcgtt gtcgcccaca actattacca gattccgggc
     2461 ggcgaggacg agacgctgcg ccgcgagcgc gatctgctgc gttcggccgg ccattcggtg
     2521 aaggaattca ttcgccgcaa cagcgaaatc gcaccggctg acatcgttgc caagatgtct
     2581 ctggctgcac ggaccgtgtg ggccggtgac agccgcaagg cgatgctgca gttcctcagg
     2641 gaagagaatc ctcacgtcgc tcactttcac aatacattcc cgctgatgtc gccttcg
//
LOCUS       DASPUL010000647         5985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_268231, whole genome shotgun sequence.
ACCESSION   DASPUL010000647 DASPUL010000000
VERSION     DASPUL010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268231"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VEJ00_14895"
     CDS             complement(<1..488)
                     /locus_tag="VEJ00_14895"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXX02501.1"
                     /translation="MEITRRHLLMLLPTAAVAWKHVLAGTPESAPNYRMTEHWWGMLI
                     DIPKCIGCGSCVRACAQENDVPDGYFRTWIERYHVADWKIERPEVESPDGGKQGFPPN
                     EESSGKNFFVPKLCNQCADSPCTQVCPVGATFVSPDGVVLVDKKYCLGCRYCVQACPY
                     GCR"
     gene            complement(488..994)
                     /locus_tag="VEJ00_14900"
     CDS             complement(488..994)
                     /locus_tag="VEJ00_14900"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXX02502.1"
                     /translation="MAPSKVNVFKDIEHLIRLALAIAIVVVAFVLVRQAVVPHSFGQF
                     GHHRGDAIAEIAARPVAFAGHQVCEGCHSDVVDQKNLGKHVVVSCEACHGALSRHAED
                     PTSVTPPKLDTAVVCGRCHEANSAKPKTFPQVVTADHSGGIACDTCHQPHKPGINVPE
                     TKIAEGRK"
     gene            1200..2027
                     /locus_tag="VEJ00_14905"
     CDS             1200..2027
                     /locus_tag="VEJ00_14905"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXX02503.1"
                     /translation="MSTKKTSNPDAARFQSGAEKYAAYLETPEGRLRLDLAFANLQEF
                     LPKATRSSQALDLGGGTGEIALRLARLGLHVTLLDTSQPMLDFAKRAAREAGVADRIA
                     LKHADAVQLSTLFPAGSFDLIVCHNILEFVDDPVAVLRSAARALRDPSGMISILVRSQ
                     PGEVLKAALVNGDLAAAERNLTADWGDEALYGGKVRLFTAESLHSMLAAASLAVTAER
                     GVRVVSDYLPSSVSRNDEYERIFELERKLGKRPEFAAVARYTQFLAHRAGRVGGDGA"
     gene            2024..3082
                     /locus_tag="VEJ00_14910"
     CDS             2024..3082
                     /locus_tag="VEJ00_14910"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXX02504.1"
                     /translation="MNDVTTGSLSSASGVIAPDAGISLGLGLTNECNLTCAFCYRDPT
                     RADRLSLNQVKAAMECLPVRSVNLGTGENGMHPDFKTILAYLQTRPVKLTITSNGHSV
                     AVLEDKELQAFHDIEFSLDYPTEAEQDGQRGPGNWALIHEQAERCLRLDIPVTIIAVM
                     MKSNYPRLADVARVAKQFNAPLRVNVYQAVRSDIYALSYEEYWDGFRRLFAETDVIAV
                     GEPLVRAMAGLPPLRAGCGVSTVRVTPRATTQPCVYWTGLGEPLSDLISAGVDIFSST
                     PFEQARTLPEACQPCEFRESCHGGCAGRRRLQGALLQPDYYCPIIRGARPQLEIRMAA
                     TRDLPKLSSSCTTVVIAR"
     gene            complement(3132..3866)
                     /locus_tag="VEJ00_14915"
     CDS             complement(3132..3866)
                     /locus_tag="VEJ00_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02505.1"
                     /translation="MTKSSRFTILGLLGCAVLVFAPSSRAQQRAPILEKVAKAYGVDS
                     FDKVEAIRYTWNGEISGLFKLSRTWEWEPKTNKVTYEGKDKDGKPVKVTYLRSELSSQ
                     PENVQKEVDPGFINDNYWALFPFHAYWDKSATVIDQGMFNLPTGGGTAELVPVKYPAD
                     SGYTPGDTWDLYVGKDGRVVYFVYHRGGAKPPSRVFATWAGYKKAGPLLFATEHRGTA
                     DGKPFHLSITDVAVKLAGSDKWVNAE"
     gene            complement(3982..5265)
                     /locus_tag="VEJ00_14920"
     CDS             complement(3982..5265)
                     /locus_tag="VEJ00_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02506.1"
                     /translation="MKLAPAWRVYLLGVMLCVALTICSHSFGDRGGPYFMGSLTLAGI
                     AYLVAVREFFAAPRFPRRVVVIGLVLAALWHIEFLRLPPGSDDDIHRYVWDGHIQRLG
                     YNPYLVKPSDPGVRGLHSPETRNLNNPDLPSPYPPGAQLFFRGVTAVQESTFALKVAF
                     VICDLLIVLVLFDLLRRNQRGAHLVLAFAWNPLLAIEVAGSGHIDIVGALLLVISVAA
                     LSRSWRSIAALTFGLAVAVKFLPIVLLPLYWKRVRIRDAALAAAVVGLLYVPFLDHGR
                     IPTGSLGTYVQSFRFNGPLFAALAQVAPPQFLAGLAVLAGLVTATWLRVRSAPPEWSP
                     EVLAWPMAATLVCAPVVFPWYLLWLVPFLTSASTLVLIVWTVGIIPTYLMWHWRALGH
                     PWAALPDWVMLVEYGCVAIAGAILALSRIRRPAVP"
     gene            complement(5262..>5985)
                     /locus_tag="VEJ00_14925"
     CDS             complement(5262..>5985)
                     /locus_tag="VEJ00_14925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HXX02507.1"
                     /translation="VRSPKSHRVLVIMAKAPRPGAVKTRLASSLSPEAVTEFYRCLLE
                     DTLALAWSLTDVEVAIMCPDFDVNELAQLAGNETNIVAQKGEGLAAGLTSVFAHFAPD
                     HRKDAGQRRIIAFNSDSPHLPRSVLEDAFETLAAHDVVVGPTHDGGYYLVGAKAPHPT
                     LFANDGMGTSSALERLLSRARELKLSVGFADAFYDIDVADDLTRLAEELRLAPSKAPR
                     TAQWLKEWHLAPAQPRTGAGKS"
BASE COUNT         1256 a         1821 c         1744 g         1164 t
ORIGIN      
        1 cggcagccat aggggcatgc ctgcacgcaa taacggcagc caaggcaata tttcttgtcg
       61 accagaacaa cgccgtcggg ggagacgaaa gttgccccta ccggacacac ctgggtgcag
      121 ggcgaatccg cgcactggtt acacaacttc ggtacgaaga agttttttcc actgctctcc
      181 tcattgggcg ggaacccctg cttgccgccg tctggcgatt ccacctcggg tctttcaatc
      241 ttccagtcgg caacgtgata ccgctcgatc catgtccgga aataaccgtc gggaacgtca
      301 ttttcctgag cgcatgcacg cacgcaactt ccgcagccaa tgcatttggg gatgtcaatc
      361 agcattcccc accagtgctc cgtcattcgg tagttcggcg cagactcagg tgttccggcc
      421 aggacgtgtt tccacgcgac agccgcagtt ggcaacagca tcagcagatg gcggcgggtg
      481 atttccatta tttcctgccc tccgcgatct tcgtctctgg cacgttgatt cccggcttgt
      541 ggggctgatg gcacgtgtca caagcgatgc cacccgagtg atcggcggta acgacctgcg
      601 gaaacgtctt cggcttggcg ctgttggctt cgtgacagcg tccacatacc acggctgtgt
      661 ccagcttggg cggtgtaacc gatgtcggat cctcggcgtg gcgggaaagc gccccgtggc
      721 acgcctcgca agaaaccacc acgtgcttgc ccagattttt ctggtccacc acgtctgagt
      781 ggcagccttc gcagacctgg tgtccggcaa acgcgacggg acgggccgcg atctcggcga
      841 tagcgtctcc tcgatggtgg ccgaactggc cgaagctgtg cggaacaaca gcttggcgaa
      901 cgagcacaaa tgcgaccacc acaatggcga tagccagggc caaccgaatc agatgttcga
      961 tgtccttaaa cacgtttacc ttgctcggcg ccactcgggc tgtctgcgcc cgaaaaaccg
     1021 catgttcttg catacgtggt attgcgttac ggaacgatga tccaagtcac aagaacctgt
     1081 gatgaaggtc actatttgat tccttcaccc gaggccgacc gccttgccgc ccatcattcc
     1141 gcattcctga gcgctgttct ccgccatccc tcctgtggta cttattgacg gtgccgcaag
     1201 tgagtacgaa gaagacctcc aatcccgacg ccgcacgctt ccagagcggc gcggagaagt
     1261 acgccgccta cctcgagacg cccgaaggac gactgcgcct tgatctcgcc tttgccaact
     1321 tgcaggagtt tctgccgaaa gccactcggt cctcgcaggc tcttgatctt gggggtggta
     1381 ccggcgaaat tgcgctgcgc ctggcacgac ttggcctgca cgtcacgctg ctggatacgt
     1441 cccagccgat gctggatttc gccaagcgcg ccgcgcggga agccggagtt gcggacagga
     1501 ttgccctgaa acacgccgac gccgtgcagt tgtcaacctt gttcccggcc ggatcatttg
     1561 atctgattgt ttgccacaat atcctggaat ttgtcgacga cccggttgct gtgttgcgca
     1621 gtgcggcgcg cgcattgcga gatccgtcgg gaatgatctc gatcctggtt cgtagccagc
     1681 cgggtgaggt gttgaaggct gctctcgtca acggcgactt ggccgcggca gagcgtaacc
     1741 tcaccgctga ttggggagac gaagctctgt acggcggcaa ggtgcgccta tttacggcag
     1801 agagcctgca ttccatgctg gccgcagcat cgctcgcggt gactgccgag cgtggggtgc
     1861 gggtcgtctc ggattatctg ccgtcgagcg tttctcgaaa tgatgagtac gagcgcattt
     1921 ttgagcttga acgcaaactg ggcaagcgac cggagtttgc cgcagtcgct cgttacaccc
     1981 agtttctggc gcatcgcgca ggccgggtcg gtggggatgg ggcatgaacg acgtcacaac
     2041 cgggtctttg tcgtccgcat ctggcgtgat cgcgcctgat gccggtatat cgctcgggct
     2101 ggggctcacc aacgagtgca atctcacctg cgccttctgc taccgcgatc caactcgggc
     2161 ggatcgcctt tcgctcaatc aagtgaaggc agccatggaa tgcctgcctg tacgctcagt
     2221 gaatctcggg accggtgaga acggaatgca tcccgacttc aagacaatcc tcgcctatct
     2281 gcagacgaga ccggtcaagc tcaccatcac atccaacggt catagtgtcg cggttctgga
     2341 agacaaggaa ctgcaggcct tccacgatat cgagttctct ctcgactacc ccacggaagc
     2401 ggaacaggat gggcagcgcg gacctggcaa ttgggcactc atccacgagc aggcagaacg
     2461 gtgcctgagg cttgatattc ctgtgaccat catcgccgtc atgatgaagt caaactaccc
     2521 gcgcctggcg gatgtggcgc gagtcgcaaa acagtttaac gctcctttgc gagtaaatgt
     2581 gtaccaggcc gttcgatcgg acatttatgc cctcagctac gaagagtact gggatgggtt
     2641 tcgccgactg tttgcagaaa ccgatgtgat cgcggttggc gagccgctcg ttcgcgccat
     2701 ggcgggactt ccgccgcttc gagccggctg tggagtgagc accgtccgag tcacgccgag
     2761 agccacgaca cagccctgcg tctattggac gggattgggc gagccactct cggacttgat
     2821 ttccgcaggc gttgacattt tcagctcaac accttttgag caggcacgga cacttcccga
     2881 agcatgccag ccgtgcgaat tccgcgaatc atgccacggc ggatgcgccg gacggcgacg
     2941 cttgcaaggt gccctactac agcctgatta ttattgtccg atcattcgcg gagcgcgccc
     3001 ccagctcgag atccggatgg ccgcaacccg cgatctgccc aaactgagca gttcctgcac
     3061 caccgtcgtg atcgccaggt agtttgatcc atgacgaacg acccgttcgc atccagcccg
     3121 gcccaattcc tttactccgc gttcacccac ttatccgagc cggccaactt cactgccacg
     3181 tcggtaatgg agaggtggaa gggcttgccg tcggcagtac cgcggtgctc ggttgcgaac
     3241 agaagaggac ctgccttctt gtagccggcc caggttgcga agacgcggct cggtggcttg
     3301 gctccgccgc gatggtagac aaagtaaacg actcggccat ccttgccgac gtaaagatcc
     3361 caggtatcgc cgggcgtgta gccgctatcc gcgggatact tcaccgggac cagttcagct
     3421 gtgccgccgc ccgtcggcag gttaaacatg ccctgatcga tcacggtggc gcttttgtcc
     3481 cagtaagcat ggaagggaaa cagcgcccaa tagttgtcgt tgatgaaacc cgggtcgacc
     3541 tctttctgca cgttctcagg ctgactgctg agctcggagc gcaggtaggt caccttgacc
     3601 ggcttgccgt ctttatcctt gccctcgtac gtgaccttgt tggtcttggg ttcccattcc
     3661 cacgtgcggg aaagcttaaa cagtccggag atctctccgt tccaggtgta gcggattgct
     3721 tcaactttgt cgaacgaatc aacaccgtag gctttggcga ccttttcgag aattggcgcc
     3781 cgctgctgcg cccgggagct tggagcgaag accagcactg cacaacccag aagcccaaga
     3841 atcgtaaaac ggctcgactt tgtcacggga ggtccctcct gaatgagttg acgaatgccg
     3901 tgttggatga cctgaaaaca ccgcgggata caacaaaaac ctgaactctg tgctccttcc
     3961 gcctgcctat gccctggggc gttagggaac tgccggccgc ctgatccggc tcaaggcaag
     4021 aatggcaccg gcgatggcca cgcacccgta ttcgaccagc atgacccaat cgggaagcgc
     4081 tgcccacgga tgcccgagcg cgcgccagtg ccacatcaag taggtgggaa tgatgcccac
     4141 agtccagacg atgagcacca gcgtcgaggc cgacgtcaga aatggcacaa gccagaggag
     4201 ataccaggga aacacaacgg gggcgcacac aagagtggcg gccatcggcc aggctaatac
     4261 ctccggagac cactcgggtg gtgcacttct tacccttagc caggttgcag tcactagtcc
     4321 ggcgagcacg gccagcccgg ctagaaactg cggaggcgcc acttgagcaa gtgccgcgaa
     4381 cagcggacca ttaaaccgga aactctgcac gtacgtaccc agcgagccgg ttggaatgcg
     4441 gccgtgatca agaaatggga catacagcag tccgacaact gccgccgcca gcgccgcgtc
     4501 gcgaatacga acccgcttcc agtagagcgg cagcaacaca atcggcagga atttcactgc
     4561 gacagccagc ccaaacgtca acgcagcaat ggagcgccaa ctgcgcgaga gtgcagcgac
     4621 agaaatcacc agcagaagtg ctccaacaat atcgatatga ccgcttcccg cgacttcaat
     4681 cgccaacaga gggttccagg cgaaggccag aaccaggtgc gctccacgct ggttgcgacg
     4741 cagaagatcg aacaggacca gcacgatcag caaatcgcaa atcacgaacg ccactttcag
     4801 cgcaaaagtg gactcctgaa ccgcggttac gccgcggaaa aacagctgcg ctcccggtgg
     4861 atatggactc ggcagatccg ggttgttcag gttgcgggtt tccggactat gcagcccgcg
     4921 aacgcccgga tcactgggct taaccagata agggttatag ccgagtctct ggatgtgtcc
     4981 atcccagaca tagcgatgaa tatcatcatc ggagcccggt ggcagccgaa gaaactcaat
     5041 gtgccacagg gcagcgagaa cgagcccaat gacgacgacg cgccgcggga atctcggcgc
     5101 agcaaaaaac tcacggaccg cgacgaggta ggcaataccc gcaagcgtca gagagcccat
     5161 gaagtacggc ccaccgcggt cgccgaagct gtgtgagcag atcgtcagcg ccacgcacag
     5221 catgacgccg agcaggtaca ctctccaggc gggggctagc ttcacgattt tcccgcgccc
     5281 gtccgaggtt gtgctggcgc aaggtgccat tcctttagcc actgcgctgt ccgtggcgcc
     5341 tttgacggag ccagccgcaa ctcctctgcc agccgagtga ggtcgtcagc gacatcgatg
     5401 tcgtagaacg cgtctgcgaa accgacggaa agcttcagct ctcgtgcacg cgacagaagt
     5461 cgctccagcg cgctgctggt gcccattccg tcgttggcga aaagcgtagg atgaggggcc
     5521 tttgcgccga cgagatagta gcccccgtcg tgcgttggcc ccacgaccac gtcatgcgca
     5581 gccagcgttt cgaatgcgtc ttcgagcacc gagcgcggca gatgcgggct gtcgctgttg
     5641 aaagcgatga tgcgtcgctg accagcgtct tttcgatgat ctggcgcgaa gtgcgcaaac
     5701 accgatgtaa ggcctgcagc aagaccctca cctttctgcg caactatgtt cgtttcgttg
     5761 cctgccagct gcgcgagctc atttacgtca aagtccgggc acatgatggc aacttccaca
     5821 tcggtcaacg accacgccag cgcaagcgtg tcctcgagaa gacagcgata gaactcagtg
     5881 acggcctccg gagaaaggct ggaagccagg cgggttttga ccgcgcctgg cctgggtgct
     5941 ttcgccatga tgaccagcac acggtgactc tttggcgaac gcacc
//
LOCUS       DASPUL010000648         4245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1079396, whole genome shotgun sequence.
ACCESSION   DASPUL010000648 DASPUL010000000
VERSION     DASPUL010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079396"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..866)
                     /locus_tag="VEJ00_14930"
     CDS             complement(129..866)
                     /locus_tag="VEJ00_14930"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXX02508.1"
                     /translation="MIPEDKINDFAKRAREGGGTNIESIILFGSAASGDFHPGLSDLN
                     LFCVLRDSSFAALQKLAPVVKWWGQQKQPPPLCMTRHELERSTDVFTIELLDMVQHHR
                     VVFGEDVLQGLHISMHQHRVQVEYELREKLILLRQHVLLADEKSSRLWDVLLHSVQSF
                     ATLFRHALIALGSEAPASRREAVQALSRQLQFDLSAIEQVLDIRERKLDQKRLNAAEV
                     CSSYLAAIEKVAAAVDRALDSDPSGRR"
     gene            complement(1005..1823)
                     /locus_tag="VEJ00_14935"
     CDS             complement(1005..1823)
                     /locus_tag="VEJ00_14935"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXX02509.1"
                     /translation="MTLRAKLRLAACVLLSLATAVLTESAFAESIKDLKPTGYVNDFA
                     HVLDQSTITQIDDICQQIDQKAHAQIALVTINTLDGNDLESYSIDLAQKWGMGGKGHD
                     RGVLILYVIQDHRARIEVGYGLEPILPDGKVGSFQREAIPLMRSEQYNQALLLVTSRV
                     AQVIADDAGVQLTNAMPRAPAQPVGQPDTGLSLGAIIFIIIVIVVVLATPLRSLLFWI
                     LFSNIGRGGGGWSSGGGGFGGFGGGGGGFGGGGGGGFGGFGGGSFGGGGASSSW"
     gene            2008..2457
                     /locus_tag="VEJ00_14940"
     CDS             2008..2457
                     /locus_tag="VEJ00_14940"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX02510.1"
                     /translation="MSKVTIENGALAQAAECAAVFRTARKHSLPYLPDLHSEDEDVRF
                     LADQVFATDTVLVALNEARHVVAFIAFGEGWVNHLYVAPGWQGRGVGRELLERAKLQF
                     PSLQLWTFERNAGALRFYEREGFRVLKHTDGSGNEEKEPDVLLRWEG"
     gene            2804..3271
                     /locus_tag="VEJ00_14945"
     CDS             2804..3271
                     /locus_tag="VEJ00_14945"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HXX02511.1"
                     /translation="MRKGKIAKPTTGRAQIVTTDAEIDRAIARAVRLREGPLVKEVEY
                     RPEPSLDMVVLKLTDGSRRMIPREDLQGLETGTPEQLARVEIVGGGTGLHWPDLAADL
                     YVPALLRGVYGTRQWMARIGRRGGSVTSAAKKRAARANGKLGGRPRRDAAAGD"
     gene            3382..3630
                     /locus_tag="VEJ00_14950"
     CDS             3382..3630
                     /locus_tag="VEJ00_14950"
                     /inference="COORDINATES: protein motif:HMM:NF025088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HXX02512.1"
                     /translation="MPTVLRHGRLRIVIYPADHRPAHVHVIGPDGEAVFNLNCPRGPA
                     ELRENYGLRRRELTIAEGAINEHIRELCRAWEDIHGDA"
     gene            3617..4039
                     /locus_tag="VEJ00_14955"
     CDS             3617..4039
                     /locus_tag="VEJ00_14955"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HXX02513.1"
                     /translation="MVTRSEIELANQKGERMRSGFPHAVAARYDRHNRKVVIELSSRL
                     TLSFSPADAQGLENARPSELEVIELSPSGFGIHFPKLDADLYVPGLLEGLMGSKKWMA
                     ARLGQLGGRSRSRAKKAASRANGKLGGRPRREAAAAGD"
BASE COUNT          908 a         1170 c         1367 g          800 t
ORIGIN      
        1 acgttgacgt attgtccgaa cacagcgaac gcgatcagtc caatcacgag cactacaaca
       61 atgacggcga tcaatgcttt gcccattggg gcctccttgc agaattcgaa ctatgctagc
      121 gaagtatgtc atcgacggcc ggacgggtct gagtccagcg cccgatccac cgccgcggca
      181 accttctcga tcgccgcgag atagctggag cagacctcgg cggcattcaa tcgtttctgg
      241 tccagcttgc gctcgcggat gtccagcacc tgctcgattg ccgacaggtc aaactgcaac
      301 tgcctcgaca atgcctgaac agcctcgcgg cgcgacgccg gagcttcgct tcccagcgcg
      361 atcagggcgt gacggaagag tgtggcaaac gactgcacgg aatgcagcag cacgtcccac
      421 agccgagagc tcttttcatc tgccaggagc acgtgctggc gtagaagaat cagcttttcg
      481 cggagttcat actcgacctg cacgcggtgc tggtgcatcg agatatggag gccctgcagc
      541 acatcctcgc cgaacacgac gcggtgatgc tgcaccatgt cgagcaactc gatggtgaag
      601 acgtcggtgg agcgctccag ttcgtggcgc gtcatgcaca gcggcggcgg ctgcttttgc
      661 tggccccacc acttaacgac cggcgcgagc ttctgcaggg cggcaaatga gctgtcgcgc
      721 aggacgcaga acaggttcag gtctgagagg ccggggtgga aatcgccaga cgcggccgaa
      781 ccgaacagaa taatcgattc gatgtttgtt ccgccgccct cgcgggcgcg tttcgcgaaa
      841 tcgttgatct tgtcttcagg gatcatagga atctttgcaa taacaccggg gctaaagccc
      901 cagtcgattt atcacccgtg acgcggcgct aaagcgccgc tcttccacgg agaccattcg
      961 cccttcgttt gcaccttagc tccggttgca cgttagatcc gttactacca actgctgctg
     1021 gcgccaccgc cgccgaagct gcctccgcca aaccctccga agccgccacc accgcctcct
     1081 ccaaatccgc cgcccccgcc tccgaatcct ccgaaaccgc cgccgccgct gctccaacca
     1141 ccgccgcccc ggccgatgtt ggagaacaga atccagaaca gcaggctgcg cagcggcgtc
     1201 gcaaggacta ccactataac gatgatgatg aagatgatgg cgcccagtga caggcctgtg
     1261 tcaggctggc cgacaggctg cgccggggcg cgcggcatgg cgttcgtgag ctgaacgccg
     1321 gcatcgtcgg cgatgacctg ggccacgcgg ctggtgacca gcagaagagc ctggttgtac
     1381 tgctcgctgc gcatcagcgg gatcgcttcg cgctggaagc tgccgacctt gccgtcggga
     1441 aggatcggtt cgaggccgta cccgacctcg atccgggcgc ggtgatcctg aatgacgtaa
     1501 agaatcagga cgccgcgatc atgacctttt ccgcccatgc cccatttctg agcaagatcg
     1561 atcgagtaac tctcgagatc gttgccgtcg agcgtattga tggtgacgag cgcgatctgg
     1621 gcgtgggctt tctggtcaat ctgctgacag atgtcatcga tctgcgtgat cgtactctga
     1681 tcgagcacat gggcgaagtc gttgacgtag ccggtgggtt tgagatcttt gatcgactcg
     1741 gcgaaggccg actcggtgag cacggccgtt gccagggaaa gaagcacgca tgccgcgaga
     1801 cgaagtttcg cgcgcagagt cacgggaaaa ttgtacagga gtgtgatgaa gcaattgcga
     1861 acaccgttgc gaatcgagag caatccgcaa tatacggatt acgatgtgct atcaaagatg
     1921 aagaaatagc acgcgcaaaa tgtgcgaagg cctcagttct tcggtgcgcg tagatgcatg
     1981 ccagcaaggt attctcattt cagtccgatg agcaaagtca cgatcgagaa cggcgctctg
     2041 gcgcaggcgg ccgagtgcgc agcggtcttc cggaccgcac gcaagcactc gctcccctat
     2101 cttccggacc tgcattccga ggatgaggac gtccgcttcc ttgcggatca ggtgtttgcc
     2161 acggatacgg tcttagttgc gctaaacgag gcgcggcatg tggtggcgtt catcgcgttc
     2221 ggtgagggct gggtcaatca tctctacgtg gctcccggat ggcaagggcg cggcgttggc
     2281 agggaactgt tggaacgagc gaaattgcag ttcccgagtt tgcagctctg gaccttcgag
     2341 cggaacgcgg gcgcgctgcg attctatgag agggaaggct tccgcgttct gaagcacacg
     2401 gacggttctg gaaacgaaga gaaggagccg gatgtgctgc tgcgctggga gggataggca
     2461 cgcgggacca aggatgacta cggattgtga tttatccggg cgaccaatgc ccgcaagaca
     2521 cttctaaacc taagccgctt aggttatact gatctatggt gtcgctgagg ttcggcgggg
     2581 tccgattcgt gctgtattcg aacgatcatc cgccgcgcca cgttcacggc ttctctggtg
     2641 agacggaagc gatcattgac tcttcgagcg gaccgaagcg ttgcccttgc agagcggcac
     2701 gacgcaatac ggccggcaaa tgccaagcga tcagacgtga agaagattct tagggctgca
     2761 gctgagcact ttgacgagct tgcggggtta tgggaggcca ttcatgagga aagggaaaat
     2821 cgctaaaccc acgactgggc gcgcgcagat tgtgaccacc gatgccgaaa ttgatcgcgc
     2881 tattgcgcgt gccgtgcgat tgcgcgaagg accgctggtg aaggaagtgg aatacaggcc
     2941 cgaaccgtcg ctggatatgg tcgtcctgaa attaaccgac gggagccgga gaatgattcc
     3001 gcgcgaggat ctgcaagggc tggagacggg aacgccggag caactcgcgc gggtagagat
     3061 tgtcggaggc gggacggggc tccactggcc ggatctggct gccgatctgt atgtcccggc
     3121 cctgttgcgg ggagtctatg gcactaggca gtggatggcg aggatcggta ggcgcggagg
     3181 atcggttacg agcgccgcga agaaacgggc ggcgcgggcg aatggaaagt tggggggaag
     3241 gccgaggcgg gatgcggccg cgggagatta ggcggtgcga gaagaactat taaggattca
     3301 aagccaaccg cgaagaacgc aaagatgcgg ttccttgaag atctattaac ctaagcgatt
     3361 gggttcatgc tacgatgagc catgccgacg gtgctgcgac atggacggct acggatcgta
     3421 atctatccag ccgaccatcg cccggcgcac gtgcatgtca tcgggccgga cggcgaggcg
     3481 gtattcaatc tgaactgtcc gcgcggccct gccgagcttc gggagaacta tgggctgcgg
     3541 aggcgcgaac tgacgatcgc cgagggcgcg atcaacgagc atattcgaga gctttgtcgg
     3601 gcatgggagg atattcatgg tgacgcgtag cgaaattgaa ctggcaaatc aaaaaggcga
     3661 acgcatgcgg agcgggtttc ctcacgcggt ggcggctcga tacgaccggc ataaccggaa
     3721 ggtcgtgatt gagctgagtt ccaggctgac gctgagtttt tcgccagcag acgcgcaagg
     3781 cttggagaat gcgcgaccat cggagcttga agtcattgag ctaagcccct ccggattcgg
     3841 aattcacttt ccgaagctcg acgccgatct ctatgttccc gggctgctcg aaggtctgat
     3901 gggctcgaag aaatggatgg cagcgaggct cgggcagctt ggcgggcggt cgcggagccg
     3961 cgcaaagaaa gcggcgtcgc gggcgaacgg gaagctgggg ggaaggccga gacgagaagc
     4021 agccgcggcg ggagattagg tcacccgaaa taacgagcac cgggagacag caccgccgta
     4081 tcataacggg tgatgcatgc acttaatctg ccaactgggc gtttggtccg aatttgcgtc
     4141 ttggtttggc tctgccttgg atgcagagga accgctgcgg cccaatggag gcaaatcgcg
     4201 actggacggc ttgcatggga tgaccgagca cctgcctcaa ccttc
//
LOCUS       DASPUL010000649         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1351104, whole genome shotgun sequence.
ACCESSION   DASPUL010000649 DASPUL010000000
VERSION     DASPUL010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351104"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VEJ00_14960"
     CDS             <1..860
                     /locus_tag="VEJ00_14960"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HXX02514.1"
                     /translation="GTILGTTNRGNPFCYKTEEAGQEVVRDISDEVIANSKKLGIDAI
                     ITIGGDGSQKIGHELFQKGMKIVGVPKTIDNDLSATELTFGFDTALHTVSDAIDKIHT
                     TAASHHRVMVVEVMGRDCGWIALAGGISGGAHVILIPEIPFSIQSVCRFISQREREGK
                     RFTIVVVAEGVKLPPELKQIARGGPVGNLVGDAISLMSNKEVRVSVLGHIQRGGSPSP
                     YDRVLATRFGVAAVDLIAEGGFGKMVALRGGSIVAVDVARAIGHLKSVDPEGELVHTA
                     RAIGIGFGD"
     gene            complement(915..>2183)
                     /locus_tag="VEJ00_14965"
     CDS             complement(915..>2183)
                     /locus_tag="VEJ00_14965"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXX02515.1"
                     /translation="SGPAGGVVGAAATARRSGFQNIISFDMGGTSTDVSLVEDEIKTA
                     NDAQIAGFPVSVPMLDIHTVGAGGGSLARFDSAGVLHVGPESAGADPGPICYGRGTQP
                     TVTDANLLLGRLQPTRFLGGDFTLDLERTRRLTHEWLQKQRSTLPLEKFAAGVVRVVN
                     STMEKAIRVVSIERGRDPRQFALVAFGGAGGLHACALAEALSIPHVIVPAFPGALSAL
                     GILASDVVKDYSRTVLWRVSKSVPNAPLEGEFAALEKAAAQDFAGEAWQGSVRYARSV
                     DVRYRGQGYELNVPLSKSLLKDFELEHHRRYGYTYANREVELVTLRLRATLKSSKGRV
                     GAAALGRPGRAKLGRLSPTQTPVLFDGKKVRAAIYSREELQRGKTYAGPAVITEYSAT
                     TVVPPGKRFGIDQANNLLIWIERRRSWPDN"
BASE COUNT          408 a          604 c          750 g          421 t
ORIGIN      
        1 gaggaacgat tctcggcacg acgaatcgcg gcaatccctt ctgctacaaa accgaagagg
       61 ccgggcagga agtggtgcgc gacatttctg acgaggtcat tgccaattcg aaaaagctgg
      121 ggattgacgc catcatcacc atcggcggcg acggctcgca aaagattggc cacgaacttt
      181 ttcaaaaagg catgaagatt gtgggcgtgc ccaaaacgat cgacaacgat ctgtcggcga
      241 cggagctgac tttcggcttt gacacggcgc tgcacaccgt ttcggacgcg atcgacaaga
      301 ttcacaccac ggcggcatcg catcaccgcg tgatggtggt cgaagtcatg ggacgcgatt
      361 gcggctggat cgcgctggcg ggcgggattt ccggcggagc gcacgtcatt ctgattccgg
      421 agattccgtt cagcattcaa agtgtgtgca ggttcatttc gcagcgcgag cgagagggca
      481 agcgcttcac gattgtcgtg gtggctgagg gcgtcaagct gccgccggaa ctgaagcaga
      541 tcgcgcgcgg cgggcccgtg ggcaacctgg tcggcgacgc catttcgctc atgtcgaata
      601 aggaagtgcg ggtgagtgtg ctcgggcaca ttcagcgcgg agggtcgcct tcgccctacg
      661 accgcgtgct cgcgacgcgc ttcggggtcg ccgctgtgga cctgattgcc gagggagggt
      721 tcgggaaaat ggtcgctctg cgcggcgggt cgatcgttgc ggtcgacgtg gcccgcgcga
      781 ttgggcacct gaagtctgtc gatccggaag gagagctggt gcacacggcg cgggccatcg
      841 ggatcgggtt tggggactga aggggattct tgattgatga ttgttgaatg agaaccgtgg
      901 tccttcagtc cgtcttagtt gtccggccag cttcttcttc gctctatcca gatcagaagg
      961 ttgttcgctt gatcgatgcc gaaacgcttg ccggggggga caaccgtcgt ggcgctgtat
     1021 tcggtgatca cggcagggcc ggcatacgtc ttacctcgtt ggagttcttc gcgggagtag
     1081 atcgcggctc gaactttctt gccatcaaac aggaccgggg tttgtgtcgg cgagagcctg
     1141 ccgagcttcg ctcggccagg gcggccgaga gcggctgccc ctacacggcc cttgcttgat
     1201 ttcagcgtgg cgcggaggcg gagggtcacc agttccactt cgcgattcgc gtaggtgtag
     1261 ccgtagcggc ggtggtgctc cagttcgaaa tctttcagca aactctttga caacggaacg
     1321 ttgagttcgt atccctgacc gcggtagcgc acgtcaacgc tgcgcgcgta acgcacgctt
     1381 ccctgccacg cttcaccggc gaaatcctga gcggcagctt tctccagggc ggcaaactca
     1441 ccctccaatg gggcattggg tacgctcttc gaaactcgcc acagaacggt tcgcgagtaa
     1501 tctttgacga catcgctggc gagaatgccg agcgccgaga gtgcgccagg aaacgcggga
     1561 acgatcacgt gcgggatgct gagagcctcg gcgagggcac aggcgtgcag gcctccggcg
     1621 ccgccgaaag cgacgagcgc gaactggcgg ggatcgcgtc cacgctcgat ggagacgacg
     1681 cggatggctt tctccatggt ggagttcaca acgcggacga ccccggcagc aaatttttcc
     1741 agcggcagag tcgagcgttg tttttgcagc cattcgtggg tgagacggcg ggtccgttca
     1801 aggtcgagag tgaagtctcc gccgaggaag cgcgtgggct gcaggcgacc aagaaggaga
     1861 ttggcatcgg tcacggtggg ctgcgtgccg cggccgtagc agatagggcc gggatcggct
     1921 cccgcggact cggggcctac atgcagaact cccgcggagt cgaagcgtgc gagcgagcct
     1981 ccgccggcgc cgacggtgtg gatatcgagc atcgggacgc tgacggggaa accggcaatc
     2041 tgcgcgtcgt tggcggtctt gatttcatct tcgaccagtg agacgtcggt tgaagtgccg
     2101 cccatgtcga aggagatgat gttctggaat ccgctgcggc gggcggtggc agcagcgccg
     2161 acgacgcctc cggcaggacc gga
//
LOCUS       DASPUL010000650         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_676175, whole genome shotgun sequence.
ACCESSION   DASPUL010000650 DASPUL010000000
VERSION     DASPUL010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676175"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..987
                     /locus_tag="VEJ00_14970"
     CDS             256..987
                     /locus_tag="VEJ00_14970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXX02516.1"
                     /translation="MAGAPNEAVTQAAFIEAKNLRKTYHVGKIEVEALRGISFTVRRG
                     EFVSVVGPSGSGKSTLFYLLGGLTRADGGSVVLDGDDLAALSDAERTRMRKRKIGFVF
                     QKFNLLPTLDARSNIAIAQEISGNGDGDPAHFQRITELLGLTKRLHHRPSELSGGEQQ
                     RVALARALINKPAVVLADEPTGNLDSKNSEIVLGMLRKSNQELGQTVLMITHNPEAAK
                     YGDRIIHMRDGQIVKPEEDPQWALH"
     gene            1179..>2480
                     /locus_tag="VEJ00_14975"
     CDS             1179..>2480
                     /locus_tag="VEJ00_14975"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXX02517.1"
                     /translation="MSNPGIPDAKIPGSNAPSPAESTESFGEILSEYQKTHSRRTEGS
                     RQLQGTVIAVTSESVFFDIGYKSEGILPLTALQGQTLKPGDKFLVSIKGRDPDGYYEL
                     SRFKAERPTDWTALERAFADKAPVLGTVTAVVKGGFSVDVGVRAFMPASRSAVRDAAE
                     MENLVGQEIRCRITKLDVTDEDVVVDRRVVAEEEERSAKDRLYSQIKEGEVVSGTVRS
                     LTDYGAFIDVGGVDALLHVGDIAWGRVNKPGDVLSVGQQIEVKVLKLGAEGDKRRISV
                     GLKQLQPHPWDAVAQKYKPGDRVRGTVTRLMEFGAFVELEPGIEGLIHISEMSWSRGK
                     VRKASDVVKPGEVVEVVILGVNAAEHRMSLGLKQALGDPWADAPQRFPVGSAIEGPVT
                     SLTKFGAFVQLAEGVEGMVHVSEISAEKRINQPADVLRVGQT"
BASE COUNT          521 a          745 c          770 g          444 t
ORIGIN      
        1 caaagtccgc atttacgacc acgactcgca agccgacgtc ttcctcacca aggggatgta
       61 ctctccggca gatttgctgg agcgcgttcg cttgctgctg gtgcgcaagc gcgggcccaa
      121 gcgcctgcag caccggacgg cacagtctgc ccagcacgcc ggcgtggcct gaagtctgac
      181 tcgcagacag ttcagccagt gtcccagggc tgcatcagcc ccgctgtacg ccttcgtgta
      241 agattcctat cagacatggc cggagctccc aacgaggcag tcacccaagc cgcatttatc
      301 gaagcgaaga atctgcgcaa gacctaccac gtcggcaaga tcgaggtcga agcgctgcgc
      361 ggcatctctt tcacggtgcg aagaggcgag ttcgtcagcg tagttggacc ctcgggcagc
      421 gggaaatcga cgctctttta tcttctgggc gggctcacgc gcgccgatgg cggcagcgtg
      481 gtgctcgacg gcgacgactt agccgcattg tccgatgccg agcgcacacg tatgcgcaaa
      541 cgcaaaatcg gattcgtatt tcagaagttc aacctgttgc ccacgctgga tgcccgctcc
      601 aacatcgcga ttgcgcagga gatttccggt aacggagacg gtgatccagc tcacttccag
      661 cgcatcacgg aactgcttgg tctgacgaag cgtctgcacc atcggccgtc ggaactttcc
      721 ggaggcgagc agcagcgcgt ggcgctagcg cgagcgctga tcaacaagcc cgcagttgtc
      781 cttgccgacg aacccaccgg caacctggac tcgaagaact ccgagatcgt gctgggcatg
      841 ctgcgcaagt cgaatcagga actgggccag accgtgctga tgatcacgca caatcccgag
      901 gcggcgaagt acggcgaccg catcatccac atgcgcgacg gccagatcgt caagccggaa
      961 gaagatccgc agtgggcgct gcactagagg cagcctgatt cacatttttc tctgcgcctc
     1021 tgccgaggtt ctctgcgacc gctgcgtgag aagaatggga tcgcagagta cgcctaggaa
     1081 tcgcttagcg cgctgaatga agcagttaca aaaccgcgat cgcaccggcg gtgatatcct
     1141 gattaccttc catcccctga agaggcttgt caccgttcat gtcaaacccc ggcattcctg
     1201 acgcaaaaat tcccggatca aatgcgccgt ctcccgccga atccacagaa tccttcggcg
     1261 aaattctctc cgagtatcag aaaacccatt cccgccgcac cgaaggcagc cgacaactac
     1321 aaggcaccgt gattgccgta accagcgagt cggtcttttt cgatatcggg tacaagtcag
     1381 aaggaatcct cccgctcacg gcgctgcagg gccagactct gaaacccggc gacaagttcc
     1441 tggtctccat taaagggcgg gaccccgacg gctactacga actctcgcgt ttcaaggcgg
     1501 agcgtccgac ggactggacc gcgctggagc gggcatttgc cgacaaggcg cccgtcctgg
     1561 gcacggtgac cgctgtcgtc aaaggtggct tcagtgtcga tgtgggcgtg cgggcgttca
     1621 tgcctgcctc gcggagcgca gtgcgcgacg ccgccgagat ggaaaacctg gtgggccagg
     1681 agattcgctg ccgcatcaca aaactcgatg tgactgatga agacgtagtc gtcgatcgcc
     1741 gcgtggtggc ggaagaggaa gagcgttcgg ccaaagatcg cctctactcg cagatcaagg
     1801 aaggcgaagt cgtaagtggc accgtgcgca gcctcaccga ctacggcgct ttcatcgatg
     1861 tcggcggagt ggacgcgctg ctgcacgtcg gcgacatcgc ctggggtcgg gtgaacaaac
     1921 ccggggacgt gctctcggtc ggacagcaga ttgaagtgaa ggtgctcaag ctcggggccg
     1981 agggcgacaa gcggcgcatt tcggttggct tgaagcagct gcagccgcac ccctgggatg
     2041 cggtcgcgca gaagtacaaa cccggcgacc gtgtgcgtgg caccgttacc cgcctgatgg
     2101 aatttggagc cttcgtagag cttgagccag gcatcgaggg cctgattcac atttcggaaa
     2161 tgtcgtggag ccgcggcaaa gtccgcaaag caagtgacgt ggtcaagccg ggcgaggtcg
     2221 tcgaggtagt gatcctcggc gtgaacgccg ccgaacatcg catgtccctg gggctgaagc
     2281 aggcgctggg cgatccctgg gccgacgcgc cgcagcgctt cccggtcggc tcggcgatcg
     2341 aaggcccggt gacgagcctg acgaaattcg gtgcattcgt gcaacttgct gaaggagtcg
     2401 aaggcatggt tcatgtcagc gagatcagcg ccgagaagcg catcaatcag cccgcggatg
     2461 tgctgcgtgt aggccagacg
//
LOCUS       DASPUL010000651         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1079997, whole genome shotgun sequence.
ACCESSION   DASPUL010000651 DASPUL010000000
VERSION     DASPUL010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079997"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VEJ00_14980"
     CDS             <1..392
                     /locus_tag="VEJ00_14980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02518.1"
                     /translation="EGVSPGQAKLTFAMARHAAVDLAQVVNAKYDPAAGDRLPAPGFD
                     TLKDALAAAGLKLRSDEAGRGKLTKLRSMYEPYVHSTGRNLMMTLPPWMHPEKLRDNW
                     QAGPWDRVIQARGLGALGRTSGTEDHF"
     gene            424..1464
                     /locus_tag="VEJ00_14985"
     CDS             424..1464
                     /locus_tag="VEJ00_14985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HXX02519.1"
                     /translation="MSSRKQKDTGENVVRIEAEALRALADRIAGPMAGAFRRAVELMF
                     SCAGRVVVTGMGKSGLIARKIAATLSSTGTPALYLHPVEALHGDLGMVVRGDVVLALS
                     ASGETEEILELLATIKRLQVPLISMTGDEICENPHFSRKERARNGAPSSISTLAAAAE
                     VALDCSIAQEACALGLAPTASTTTMLALGDALAVTLSERRGFKEEDFANLHPGGKLGK
                     RLARVESLMHSGDTLPRVTPKTKMPDVIYEMSRKKLGVTAVVDGKKLVGVISDGDLRR
                     LLEKRGKDVMDLTAGAAMTRNPKTIAAREFAATALAMMEEKKITSLMVVDKRGKLEGI
                     VHLHDLWTTELV"
     gene            1557..1766
                     /locus_tag="VEJ00_14990"
     CDS             1557..1766
                     /locus_tag="VEJ00_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02520.1"
                     /translation="MYHYDPNTALEELTEDATLPNPVHVRDMILRKHPSTDKALELNR
                     KFVEYQKLFGEAQKLGKDILKDLAA"
     gene            complement(1776..2459)
                     /locus_tag="VEJ00_14995"
     CDS             complement(1776..2459)
                     /locus_tag="VEJ00_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02521.1"
                     /translation="MQHNADFPLWLLLLGITVVALVVAWGIRAARQRTRDMTAIAQQL
                     GLTFVGDHWRGPTLSPRFKMTLLQRMHGRFRNVMIGSSGGLEVAVFDYTYSQGKSSVT
                     LTLACFSQDADLSPFELRPEGFFDKIGDAILHNDLDFDSHPDFSNRYHLRSPDEKHTR
                     MLFSPSLLTYFEQIPLDRKWHVEASGPTLVLYEFRGRVATDNIPNFLNDTSSIARTIL
                     SSSSALSNS"
     gene            2636..>2914
                     /locus_tag="VEJ00_15000"
     CDS             2636..>2914
                     /locus_tag="VEJ00_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02522.1"
                     /translation="MHRWSELFIPTLREAPADAEVASHKFLVRAGYIRQLAAGIYSYL
                     FLGNRSFNKIVAIVREEMDRVGQEFYLPALHPREIWEASGRWTVMGDNL"
BASE COUNT          614 a          723 c          983 g          594 t
ORIGIN      
        1 ttgagggagt gtctcccgga caggccaagc tgacgttcgc gatggcgcgg catgcggccg
       61 tggatctggc acaggtggtg aacgcgaagt acgatccggc tgccggcgac cggcttccgg
      121 cccccgggtt cgacacgctg aaggacgcct tggcggccgc cgggctgaag ctgcggagcg
      181 acgaggcagg gcgcgggaaa ctgaccaaac tgcgctcgat gtatgagccc tacgtccact
      241 cgaccggccg caacctgatg atgacgctgc cgccgtggat gcatccggag aagttacggg
      301 acaactggca ggcggggccg tgggatcgcg tgattcaggc gcgcgggttg ggggcgctgg
      361 ggaggacttc ggggaccgaa gatcactttt gagcgagagc ggttcagcgt gagtaggttt
      421 tccatgagtt caagaaaaca gaaagatact ggcgagaacg tggtgcggat cgaggccgaa
      481 gcactgcgtg ctctagcgga ccgcattgcc gggccgatgg caggggcgtt tcggcgggcg
      541 gtggagttga tgttctcatg cgcgggacgc gtggtcgtta ccggcatggg caagagcggg
      601 ctgatcgcgc gcaagattgc cgcgacgctg agctcgacag ggacgccggc gttgtatttg
      661 catccggtgg aggcgctgca cggagatttg ggcatggtcg ttcggggcga cgtcgtgctg
      721 gcgctttctg cgagcgggga gacggaggag attctcgagc tgctggcgac gatcaagcgg
      781 ctgcaggtac cgctgatctc gatgacgggg gatgagattt gcgaaaatcc ccacttctcg
      841 cgtaaagaac gcgcgagaaa tggggcaccc tcgtccatct cgacgcttgc ggcggcggcg
      901 gaagtggctc tggattgttc gatcgcgcag gaggcgtgtg cactggggtt ggcgccgacg
      961 gcttcgacca caaccatgct ggctttgggc gatgctttgg cggtcacgtt gagcgagcgc
     1021 cgggggttca aggaagagga ttttgccaac ctgcatccgg gcggaaagtt gggaaagcgg
     1081 ctggcgcggg tggaatcgtt gatgcattcc ggcgatacgt tgccgcgggt gactccgaag
     1141 accaaaatgc cggacgtgat ctacgagatg tcgcgcaaga agctgggtgt gacggcggta
     1201 gttgacggga aaaagctcgt gggggtcatc agtgatggag acctgcgccg gctgctggag
     1261 aaacgcggaa aagacgtgat ggatctgacg gcgggcgcgg ccatgacgcg caatccgaag
     1321 acgattgcgg cccgtgagtt tgctgccacg gcgttggcta tgatggaaga gaagaaaatt
     1381 acgtccttaa tggtcgtgga taagagaggg aagctcgaag ggattgtgca tctgcacgat
     1441 ctgtggacta ccgaactggt ctagtggatt tgaaatcttg taatcgggaa atttgtaatt
     1501 ttgaatcgga acacagggta aattacacaa ttacccgatt acaaatcgag gttgccatgt
     1561 atcactacga tcccaatacg gcactggaag aattgactga ggatgcaact ttgccaaacc
     1621 ccgtgcatgt gcgcgacatg attctgcgca agcatccgtc gacggataaa gccctggagc
     1681 tgaaccgcaa gtttgtggaa tatcagaaac tgtttggcga ggcgcagaag ctggggaagg
     1741 acattctgaa ggacctggcg gcctagcgac acttttcagg agttgctgag cgcgctggag
     1801 ctgctcagaa tcgtcctcgc aatcgaagac gtgtcattca ggaaattagg gatgttgtcg
     1861 gtagcgactc tccccctgaa ttcgtatagg actagagtgg gtcccgaagc ttccacgtgc
     1921 catttcctat ccagcggaat ctgctcgaaa taagttaaaa gacttgggct gaagagcatc
     1981 cgcgtatgct tttcatccgg gctgcgcaga tgatagcggt tggaaaaatc cgggtgggag
     2041 tcgaaatcca ggtcgttatg caggatagcg tcgccgattt tgtcgaagaa gccctctggg
     2101 cgcagttcaa agggagacag gtctgcatcc tgcgagaagc aggcgagagt gagcgtaacg
     2161 ctgctcttgc cttgtgagta ggtgtagtcg aatacggcga cttcgagccc gccgcttgag
     2221 ccgatcatta cgtttctgaa tcgcccatgc atgcgttgca gcagggtcat cttgaagcgc
     2281 ggcgagagcg tgggcccgcg ccaatgatcc ccgacgaagg taagtcccag ttgctgcgcg
     2341 atggcagtca tgtctcttgt gcgttgccgt gccgcacgaa tgccccatgc aaccacgagc
     2401 gctacgacgg tgatgccgag aagaaggagc cacagcggaa aatcggcatt gtgttgcatg
     2461 ggaaccgtcc agtgcgggaa tagtagattg caaacggccg cgattcaatt gtgattccgg
     2521 attctcctgc caagtctcgc gtcgcaatca tttacaataa gcaattccgc tgcgggcttc
     2581 aggcgcgcat gtggttctgc gctcggggct tgcgactcac gactcgagaa tctctatgca
     2641 tcgttggtca gaactgttta ttcctaccct gcgcgaggcg ccggcggacg ccgaggtcgc
     2701 ttcgcacaag tttttggtac gggcgggcta tatccggcag ctcgcggcag gcatctactc
     2761 gtatctcttc ctaggcaacc ggtcgtttaa caagatcgtg gccatcgtgc gcgaagagat
     2821 ggaccgggtc gggcaggaat tctatttgcc ggccctgcat cctcgggaaa tctgggaagc
     2881 gagcgggcgc tggacggtga tgggcgacaa cctc
//
LOCUS       DASPUL010000652         8297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1351961, whole genome shotgun sequence.
ACCESSION   DASPUL010000652 DASPUL010000000
VERSION     DASPUL010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351961"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VEJ00_15005"
     CDS             <1..578
                     /locus_tag="VEJ00_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02523.1"
                     /translation="SMRKFAILMPLALVLAVSGCGSNTPPGTVTTTTSGNWEARLIGG
                     TGPSSQLNFVTTFTVTNTNGGSSELDIPGFSFFNSGPCFAVGTNTENKSGSATITVQS
                     NGQVTGNMTYSVTSVAANGVTGGNNLTLTSQTNGFTGTFTGSSPTTGSISNGVVVGTW
                     TLDSSDPNCTGNATKTGTFVMCQGNTTCTPP"
     gene            682..1704
                     /gene="lpxD"
                     /locus_tag="VEJ00_15010"
     CDS             682..1704
                     /gene="lpxD"
                     /locus_tag="VEJ00_15010"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HXX02524.1"
                     /translation="MTVRVRPRSVQAIAEAVGARLIGDGRAEVTGVASIVSASVSDLV
                     FVENEKYLDAALQSRAGAVIAGEFAAGARCDRPLLVSDHPKLAFARAAKLLSSAEPHA
                     EMAKAIVHATAVVHPSAMLGAGVAVEENAVVGERAQIGDRTRIAAGCSIGAGVKIGSD
                     CEIYPKVTIYPGTTLGNRVIVHAGAVLGSDGFGYVRDGKTGHYEKFPQVGWLVIEDDV
                     EVGANTTIDRGALDETRIRRGAKLDNLVHIGHNCQIGEDVVIAAQTGLSGSIVIENGV
                     VLGGQVGIGEHARIGEGVMVGGQGGILPNKVLRGKGVAFWGTPAQPLREYLKQLAALA
                     RLAKRD"
     gene            1708..2316
                     /locus_tag="VEJ00_15015"
     CDS             1708..2316
                     /locus_tag="VEJ00_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02525.1"
                     /translation="MALLVIGGHSRSVGKTSVVAGLISALPEWQWTAVKITQYGHGIC
                     SAQGEPCDCATADHSWAISEEHDRSGQSDTSRFLVAGAERALWVRTEQGRLAEAMPAL
                     RGKLADARNVIIESNSVLKFLRPDLYLTVLDPATADFKNSAREFLDRANAVIIHRSTD
                     DTTWESVSLKPVKDRPVFLITPPRYVTPEIADFVRSSLRIPV"
     gene            2419..2688
                     /locus_tag="VEJ00_15020"
     CDS             2419..2688
                     /locus_tag="VEJ00_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02526.1"
                     /translation="MKSRIAMWAGAGFSVAVGWALYAYATHPPALTSADPMLALAEIT
                     CPIAFASMHFHFSVSLYACLITNAATYGLVGLAIKSVRRGLNPAR"
     gene            2752..4350
                     /gene="lnt"
                     /locus_tag="VEJ00_15025"
     CDS             2752..4350
                     /gene="lnt"
                     /locus_tag="VEJ00_15025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HXX02527.1"
                     /translation="MRRIHSSAWLLVLLSAVLQVLIFPLPGLSVLSWLALTPLILALL
                     RTQSTGELEVIDSIRLGPASPGQAFVLGYVCGILWYAGTCYWIYDTMRQYGGLSAIWA
                     MVALFLFCCYLGLYHGLFGLLISLLAGKRRDYRWTLATAPFLWVAVELARTRITGFPW
                     NLLGIAQVNNVAMCRIASWTGVYGISLEIVLVNVALAAAFLVPRQRRGAMLAAALAAG
                     AVLQAGSLIEAPAAKADRTALLVQQNIPVAAEWTPEYFQRTLTELTDLTGRSAAQSSA
                     RVDLIVWPESPAPFFTNDPRFRAAVSDMARDTKAWTLAGAIGTSTTARGGEENSPVFN
                     SAALVSPAGDWAARYDKVHLVPFGEYLPFPRLFSFAGGLTKEVGTFGRGASRAALDAG
                     DMKLGVFICYESVFPDEVRQFANNGAQILVNISNDGWYGDSGAYAQHLNQTRMRAIED
                     DRWLLSAADTGVTASIDPWGRVAARIPRKQRTTLLAPYALISTATFYTRHGDWFAFAC
                     AIISLGALFMRFLSSRHRKVDASP"
     gene            4393..5448
                     /gene="prfB"
                     /locus_tag="VEJ00_15030"
                     /pseudo
     CDS             4393..5448
                     /gene="prfB"
                     /locus_tag="VEJ00_15030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            5535..6116
                     /locus_tag="VEJ00_15035"
     CDS             5535..6116
                     /locus_tag="VEJ00_15035"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXX02528.1"
                     /translation="MFAAEKHGGQARKASTIPYITHLMGVASLVLEAGGDEDLAIAAL
                     LHDVVEDCGGAPMLKEVRRRFGARVAKVVDGCTDSDTYPKPPWRERKENYIRHLKKAD
                     AATRLVSAADKLNNIRSILADYREIGESIWSRFNGGREGTLWYYRTLRDEFLRHKMNR
                     ITRELELAVNELESLTNAQSISISPGVQSFREA"
     gene            6120..7385
                     /locus_tag="VEJ00_15040"
     CDS             6120..7385
                     /locus_tag="VEJ00_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02529.1"
                     /translation="MAQRVIDQIRASKLPSNMMQFAARGAMQVPAAENIEILVHLARH
                     NKVFGDLARMTLAGWDEKACLGAASDPQTPREVLEYFISPDNLRPKLLPALLENPSVP
                     EAELVKLALAGLRETIEVMLKSARIQASSGLLEALRANPHLKKEEAAEIEKRSGTKPE
                     AAAEVLSEAETAPGQQAAAETAGAGQEEAAAEGGEAGDDAEVSAYLKEHSEEIAAEGE
                     KPFQAIGGIVELLGPDYFPVTQKAEAPEPEASAVAPAHAPAGPAAPKPAAKKGQPEGE
                     PRRQNTLQKINSLDVKGRIQLALKGNKEERSILIRDGTKVVALAVLEAPKLSDGEVEK
                     FASQKNVLEAVLRQIPLKRRFMKNYIVVRNLVANPRAPLDVGLPLMKNLLIQDLKNLS
                     TNKEVSETIRKLALKMFKQKAEDASRKRS"
     gene            7394..7807
                     /locus_tag="VEJ00_15045"
     CDS             7394..7807
                     /locus_tag="VEJ00_15045"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXX02530.1"
                     /translation="MALSSPSDPITDLLKRSKTIAVVGLSRNPLRPSHGVSAYMQSHG
                     YRIIPVNPRIEECLGEKAYASLLDVPEKIDIVDIFRRSEFVEDIVDQAIQLRVPAVWM
                     QEDVINEKAATKARHAGILVVMDRCILKEHRARFV"
     gene            complement(7812..>8297)
                     /locus_tag="VEJ00_15050"
     CDS             complement(7812..>8297)
                     /locus_tag="VEJ00_15050"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02531.1"
                     /translation="PLYIARRVVRMAVEDIGLADPNALSLCMAARDAVDFIGMPEGNL
                     ALAQAVVYLSAAPKSNALYTAYGEVQQDVEQTAADPVPLHLRNAPTALMKGLGYGRGY
                     EYAHDIEGKVADMECLPENLRGRVYYQPTSEGIEKRIRERLEEIKRLRLGKRSSHQSD
                     A"
BASE COUNT         1696 a         2377 c         2629 g         1595 t
ORIGIN      
        1 ggtccatgag gaagttcgcc attttgatgc ccctggcgct ggttctggcg gtaagcggat
       61 gcgggagcaa cactccgccc ggcacggtga caacgacgac cagcggaaac tgggaggccc
      121 ggctgattgg gggtaccggc ccctccagcc aactcaattt cgtgaccacg tttacggtga
      181 ccaataccaa cggaggcagc agcgagctcg atattcccgg cttcagtttt ttcaactccg
      241 ggccgtgctt cgctgtgggg acaaatacag agaacaaaag cggttcggcc actatcacgg
      301 ttcaatcgaa cggccaggtc accgggaaca tgacttacag cgtgacctcg gtagcggcca
      361 atggcgtgac cggtggaaat aacctcacgc tgacgagcca gacgaacggc tttaccggaa
      421 cttttaccgg atcatcgcca acgacaggaa gcatcagcaa cggggttgtc gtgggtacct
      481 ggaccctgga cagcagcgac cccaactgca ccggcaacgc caccaagaca ggcacgttcg
      541 tcatgtgcca gggaaacacc acctgcacgc ccccgtagca tagcggcgca gaccgcgaag
      601 tcaattctca gcatctacag gcctcctcgt gccttggcat gccctttctt ccgcttgccc
      661 gagtgctata cagtgtgtgc catgacggtg cgcgtaaggc cgcgttcggt gcaggcgatt
      721 gcggaggcgg tgggtgctcg cctgatcggg gatggccgcg ctgaagtaac gggcgtggcc
      781 agcattgtct cggcctcggt ctcggacctt gtcttcgtcg aaaatgaaaa atatcttgac
      841 gccgccttgc aatcccgggc tggtgctgtg atcgcgggag agttcgcggc gggcgcgcgg
      901 tgcgaccggc cgctgctagt cagcgatcat cccaaacttg cgtttgcgcg ggcggcgaag
      961 ttacttagca gtgctgagcc gcacgcggag atggcgaaag cgatcgtaca tgcaaccgcg
     1021 gtggttcatc cctccgccat gctgggcgca ggggttgcag tggaagaaaa cgccgttgtc
     1081 ggcgaacggg cgcaaatcgg cgaccgaact cggattgcgg caggctgctc gatcggggcg
     1141 ggcgtaaaga tcggcagcga ctgcgaaatc tatcccaagg tgaccatcta tccaggcacg
     1201 accctgggta accgcgtgat cgtacacgcc ggagccgtgc tgggcagcga cggcttcggc
     1261 tacgtgcgcg acggcaagac cgggcactac gaaaaattcc cgcaggtggg gtggctggtg
     1321 atcgaagatg acgtcgaagt cggcgccaat acaaccattg atcgcggcgc gctcgatgaa
     1381 acccgcatcc gccgtggcgc caagctcgac aatcttgtgc acatcggtca caactgccag
     1441 atcggggaag acgtcgtaat cgcggcgcag accggccttt cgggaagcat cgtgattgaa
     1501 aacggagtgg tgctgggagg gcaggtcggc atcggcgagc atgcgcgcat tggggaaggg
     1561 gtcatggtgg gcgggcaggg cgggattctg ccaaacaaag tgctacgcgg caagggtgtg
     1621 gccttctggg gaaccccggc gcaaccgctg cgcgaatatt taaagcagtt ggcagccctc
     1681 gcacggctgg ccaaaaggga ctgaatcgtg gcactcctcg tcatcggcgg gcattcgcgc
     1741 agcgtgggaa agaccagcgt ggtggctggc ttgatttcgg cgttgcccga atggcaatgg
     1801 accgcggtga agatcacgca gtacggtcac gggatttgct ccgcccaggg tgagccgtgc
     1861 gactgcgcca ccgccgatca ctcgtgggcg atttccgaag agcacgaccg ctcgggccag
     1921 tcggacacgt cgcggtttct cgtggccgga gcggaacggg cgctgtgggt gcgcactgag
     1981 caaggacgcc ttgccgaggc catgcctgcg ttgcgcggga agttggctga cgcgcggaat
     2041 gtcatcatcg agtcgaacag cgtgctgaag ttcctgcgac cggatttgta tctcaccgtg
     2101 ctcgatccgg cgaccgccga tttcaagaat tccgcgcgcg aatttcttga ccgcgcgaac
     2161 gccgtaatca ttcaccggtc gacggacgac acgacttggg aatctgtgtc actgaaaccg
     2221 gtgaaggatc ggccagtctt cctcatcacg ccgccgcggt acgtcacgcc agaaatcgcg
     2281 gactttgttc gctcaagcct gcggattccg gtgtgagaac gatctgggtc gtacgtcgga
     2341 cacatagtgg ttgcggcctt gcgcttttga tgcgaccatc agatcacatg atctagttcg
     2401 acggttggag gcggcggcat gaaatcccga attgcgatgt gggcgggcgc gggtttctcg
     2461 gttgcggttg gttgggcgct gtacgcctat gcaacgcatc ctccggcgct gacgtctgct
     2521 gatcctatgt tggctcttgc ggaaatcact tgcccgatcg cgtttgccag catgcacttt
     2581 cacttcagcg tttctttgta tgcgtgtctg atcacgaacg ctgctacata cgggctggtt
     2641 ggcttggcca ttaagagcgt gcgacgcgga ctcaacccgg cccgatagat cgaaaccaac
     2701 ctgcgcaacg aaccgctcct gttttgctga cccattttgc tatccttgcc cgtgcggcga
     2761 attcactcga gcgcctggtt gctggtgctg ctgtcggcgg tcctccaggt gctcattttc
     2821 cctctacccg gactgtctgt gctttcctgg ctggctctga cgccgctgat cctggctctg
     2881 ctgcgcacgc agtccacggg cgagcttgag gtcatcgatt caattcggct gggtccggcc
     2941 tcgcccggcc aggcatttgt gctgggttac gtgtgcggga ttctgtggta cgcgggtacg
     3001 tgctactgga tttacgacac gatgcgccag tacggcggac tcagcgcgat ctgggcgatg
     3061 gtagcgctgt ttctcttttg ttgttacctc ggcttgtatc acgggttatt cggcctgctc
     3121 ataagcctgc tcgcgggaaa gcggcgagac taccgctgga cattggcgac cgcacctttc
     3181 ttatgggtcg cagtggagct ggcgcgcacg cgaatcaccg gatttccttg gaacctgctg
     3241 ggtatcgcgc aggtgaacaa cgttgccatg tgccggatcg cgagctggac gggggtctac
     3301 gggatctcgc tggagatcgt gctggtgaat gtggcgctgg cggcggcatt tctggttcca
     3361 cgtcaaagga ggggagcgat gctggcggcg gctctggcgg caggcgcagt gctgcaggct
     3421 ggaagtctga tcgaggctcc ggcggcgaag gcagaccgca cggccttgct ggtgcagcag
     3481 aatattccgg tcgcggcgga gtggacgccg gaatactttc agcgaacatt gactgaattg
     3541 acggatctga ccggtcgttc tgcggcccag tcatcggcga gagtcgattt gatcgtgtgg
     3601 ccggagtcac cggcaccgtt cttcacgaac gatccgcgat ttcgcgctgc cgtcagcgac
     3661 atggcgcgcg atacgaaggc gtggacgctg gcgggggcaa ttgggacttc aacgaccgcc
     3721 cgcgggggcg aggagaattc tccggtgttc aactctgcag cactggtcag ccccgccggt
     3781 gactgggccg cgcgctatga caaggtgcat ctcgtgccgt ttggcgaata tctcccattt
     3841 cctcgcctgt tttcgtttgc aggagggttg accaaagaag ttgggacgtt cgggcgaggc
     3901 gcgtcgcgtg ctgcgctcga cgcgggcgat atgaagttgg gagttttcat ctgctacgag
     3961 tctgtctttc cggatgaggt tcgccaattc gcgaacaatg gagcgcagat attggtaaac
     4021 atttcgaacg acggctggta tggcgacagc ggcgcttatg cgcagcactt gaaccagacg
     4081 cgcatgcggg ccatcgaaga cgatcgctgg ctgctcagcg ctgccgatac gggagttacg
     4141 gcatcaatcg atccctgggg gcgagtcgcg gcgcggattc cgcgcaaaca gcgaaccacg
     4201 ctgctggcgc cctatgccct catctccacc gcaactttct acacccggca cggcgactgg
     4261 ttcgctttcg cgtgtgcgat aatttctctg ggagcgctct tcatgcgctt cctatcttca
     4321 agacaccgca aggttgacgc atccccatga gtgtagagct ggaacaggaa tacgaaaagc
     4381 tgggcaagaa ggtccacgaa cttcgggagt atctttgacg cggagaaaat ccgtccgcag
     4441 ctagccgata ttgaaaagca ggccgccgac ccgagtctgt ggtcaaatcc gcagaaatcg
     4501 cagcaggtga tgcgcgagaa gaagcggctg gagcacgtgc tctccaccga cgccgatctg
     4561 gcgcgacgca gcgacgatat tgacgcctat ttcgatctgg cgaaagaggg cgaaaacgtc
     4621 gactccgatc tgcagcgcga gatcggctcc ctgcgcgaac tcgtcgacaa actcgagacc
     4681 gagacgttgc tctcgggtga gaacgacgcg cgcaacgcga tcctgaccat ccatcccggc
     4741 gccggcggca ccgagtcgca ggactgggcc gacatgctgc tgcgcatgta tctacgctgg
     4801 gccgagcggc agggattcga aaccgtgctc tacgactatc agccgggtga agaagcgggg
     4861 ttgaagtcgg ccacctttgc tgttaatgga gaatatgctt tcgggttgct gcagagcgaa
     4921 atcggtgtgc accggctggt gcggatttcg cccttcgacc aggccaaacg ccggcacact
     4981 tcgtttgcca gcgtgttcgt atcgccggaa atcgacgaga gcattgaggt cgtcatcaag
     5041 cctgaggaga ttcgggtcga cacctaccgc tccagcggca agggcggaca gcacgtcaac
     5101 accaccgact ccgccgtgcg catcacgcac attccgacgg gcctggttgc cagctgccag
     5161 aacgagcgtt cacagcacaa gaaccgcgaa cgcgccatga gcatgttgcg gtcgaagctc
     5221 tacgagtttg agatggagaa gaagcgcgag gccacaaaga agatcgagga ctcaaagctc
     5281 gacatcgact tcgggtcgca gattcgctcg tacgtgctgc agccctaccg gctgatcaag
     5341 gatcaccgga cgaagaccga agtcggcgac gtggatcgcg tactggacgg ggatttgcaa
     5401 ccgttcattc gcgcgtacct gctggcacgg cggggagaga aatcgtgaag gccgcaccag
     5461 cagtaaataa caggaataaa ccactgtggc cagagggggt ccagaagtta ggacgtcgtt
     5521 tccttcgggc ctttttgttt gcggccgaga aacatggcgg ccaagcgcgc aaggcctcta
     5581 ccatccctta catcacgcat ctgatgggcg tggcttcgct ggtgctggag gcgggcggcg
     5641 acgaggatct ggccatcgct gcgctgttgc acgacgtggt cgaagactgc ggcggtgcgc
     5701 ccatgctgaa ggaagtccgc cggcgattcg gagcgcgtgt ggccaaagta gtggacgggt
     5761 gcacggattc ggatacatat cccaaaccgc cctggcgcga gcgcaaggaa aactacatcc
     5821 gccatttgaa gaaggccgat gccgctacgc gcctggtttc ggcggccgac aagctcaaca
     5881 atattcgctc tatcctcgcg gattatcgcg agataggtga atccatctgg tcacggttca
     5941 acggcggacg cgagggtact ctttggtact atcggacttt gcgggatgaa tttctccggc
     6001 acaaaatgaa tcgaattacc cgcgaactgg aactggcggt gaatgagctc gagtcactga
     6061 caaacgcgca atcaatctcc atcagcccag gagtgcaatc gttccgcgag gcttgacgca
     6121 tggcgcagcg cgttatcgat caaatccgcg cttcgaagct gccctccaac atgatgcagt
     6181 ttgccgcccg gggcgcgatg caggtgcctg ctgctgagaa cattgaaatc ctcgttcacc
     6241 tggcgcgaca caacaaggtc ttcggcgacc tggcccgcat gacgcttgcg ggatgggacg
     6301 agaaagcctg tctgggggcg gcttccgacc ctcagactcc gcgcgaagtg ttggaatatt
     6361 tcatatcccc cgacaatctg cgtccgaaac tgctgccagc tctcctggaa aatccttccg
     6421 tgccggaagc ggagctggtc aagctggcgc ttgccggttt gcgggaaacg attgaggtga
     6481 tgctgaaaag cgcgcggatt caagcttcca gcggcctact agaagcgctc cgggcgaatc
     6541 cgcatctcaa aaaggaagag gcggcagaga ttgaaaagcg atccggcaca aaacccgaag
     6601 ctgccgccga agtgctctcg gaagcggaaa ccgcaccggg tcagcaagcg gctgctgaga
     6661 cggccggcgc cgggcaggag gaggcggcgg cggaaggtgg cgaagccggg gacgatgctg
     6721 aggtttccgc gtatctcaag gaacactcgg aggagattgc agcagagggc gagaagccat
     6781 ttcaggcgat cgggggaatc gtcgagttgc tggggccgga ctattttccg gtcacgcaaa
     6841 aggcagaagc acctgagcct gaagcttctg ctgtcgcacc cgctcatgcg cctgcgggac
     6901 cggcggcgcc aaagccggct gcgaaaaaag gtcagcccga aggcgaaccg cgccgccaga
     6961 acacgctgca aaagatcaac tccctcgatg tgaaggggcg cattcagctc gcgctcaagg
     7021 gcaacaaaga agaacgctcc atcctgatcc gcgacggaac caaggtagtc gcgctcgccg
     7081 tgctcgaagc gcccaagctc tccgacgggg aagtggaaaa attcgccagc cagaaaaatg
     7141 tgctggaagc cgtgctgcgt caaatccccc tgaaacgccg tttcatgaag aattacatcg
     7201 tggtgcggaa tctggtggcg aacccccgcg cgccgctcga tgtgggcctg cccctgatga
     7261 aaaacctgct tatacaggac ctgaagaatc tttccaccaa caaagaagtc tcggaaacca
     7321 tccgtaaact ggcgttgaag atgttcaaac agaaggcgga agacgccagc cgaaagaggt
     7381 cataggaggg gccttggctc tctcatcgcc gtcggatccg atcaccgacc tgctgaagcg
     7441 ctccaagacc atcgccgtgg tgggactttc gcgcaatcct ctgcgaccca gccacggcgt
     7501 gtccgcctac atgcagagcc acgggtatcg catcattccc gtgaatccgc ggatcgaaga
     7561 gtgcctggga gaaaaagctt acgcgtcact gctggatgtg ccggagaaaa tcgacatcgt
     7621 ggatatattc cggcggtccg agtttgtgga agacattgtc gatcaggcaa tccaactcag
     7681 ggtgccggcg gtctggatgc aggaagatgt aatcaacgaa aaggccgcaa ccaaggcgcg
     7741 gcatgccggg atactggtcg tgatggaccg ctgcatcctg aaggagcatc gcgcgcgatt
     7801 cgtgtgatcc actacgcatc ggactggtgc gagctccgtt tgcccaggcg caaccgtttg
     7861 atttcctcca gccgctcgcg gatgcgcttt tcaatgcctt cgcttgtggg ctggtagtaa
     7921 acgcggccgc gcaggttttc gggcaggcac tccatgtcag cgaccttgcc ctcgatatcg
     7981 tgcgcgtact catagccccg gccgtacccg aggcccttca tcaacgctgt cggagcgttg
     8041 cgcaggtgca atggaaccgg gtcggcggca gtttgttcga catcctgctg cacctcgccg
     8101 taagcggtgt aaagcgcgtt cgattttggc gcggccgaga gatagaccac ggcctgagcc
     8161 agcgccagat tgccctcggg catgccgata aaatcgaccg cgtcgcgagc cgccatgcac
     8221 aacgacagcg cgttgggatc ggccaatccg atatcttcga ccgccatgcg caccacgcgg
     8281 cgcgcgatgt aaagggg
//
LOCUS       DASPUL010000653         4521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1627002, whole genome shotgun sequence.
ACCESSION   DASPUL010000653 DASPUL010000000
VERSION     DASPUL010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627002"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..423)
                     /locus_tag="VEJ00_15055"
     CDS             complement(43..423)
                     /locus_tag="VEJ00_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02532.1"
                     /translation="MPAAPGATKAVGMVPDELKFEPKSKGLATPRKKKPKELAVITEC
                     CTGCAGSPACVEYCPVEDCMFWVPDEDHPPFGRIAVDPILCIGCKKCLSKGPDGCFLD
                     GCPWDAIVMVDTVEVEKEVGVMAI"
     gene            546..1028
                     /locus_tag="VEJ00_15060"
     CDS             546..1028
                     /locus_tag="VEJ00_15060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXX02533.1"
                     /translation="MAGKSLNRVQLIGNLGKDPEIKYTPQGTPVAKLAIATNERFKDK
                     SGEWQDRTEWHNVVLWQRLAEIAGEYLKKGGKVYIEGRLQTRSWDDKQTGQKKYMTEV
                     VASDLILLSGRGEGGGGGDYSSSSRAAAASGGNNFDQRVPEPEHATASGPITDEDIPF
                     "
     gene            1228..>4521
                     /locus_tag="VEJ00_15065"
     CDS             1228..>4521
                     /locus_tag="VEJ00_15065"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXX02534.1"
                     /translation="MRFTHAKLTPIAHTCPRVTLVLVLLCSAVSAAHAQSLNSTSVSF
                     GNVVQNTTSAPKTVTLTNSQAVPLVISSITVSGDFAETSKCPISPNTLAAGATCQIQV
                     TYTPTVLASESGTLAVNDNATNTPQTAQLSGTGVPPVGVTPASYSFSNQFVGTTSAAR
                     VVAVNNYQTTPLTIASIAATGDFTVTTTTCPLAPNTLAAKTTCTVSVAFAPIAAGTRK
                     GTLTVTYNASNSPQSVALSGTGVLPVSVTPGSVTFSGQHPGNTSNPTNITLKNGQSTP
                     LTISAISTSGDFAESSTTCPLSPNTLASAASCTISVTFTPTTLGTRTGSLTITDTAST
                     SPQIVTLSGSGVLTGLNTITVTPSNPSMFVGNQQQFTATANFSNGSTLNITSWVTWFT
                     SDSAQAQVTSGGLVTALAPGKVLIVAKYLQSSGSTTANVSAKNLTSITVTPGSSSLPV
                     GAYQQFSALLTYSDGSTQNTTSDVAWSSSSKAVASINATGMANASSIGSTTIKATLGS
                     VSGSTTFTVFQPSCAAAPAGLVGWWTGDGNTVDLGGQNSGTLQNGASYGAGEVGQAFS
                     FTGNGASVLVNSPLYSPPAGTLSFWFMPTGSGALTGGYAGGQNRAPGLLIDSSNNLNW
                     EYGNLSSQSIGQVNLNQWNHAALTYSTSNSQTSVSVYLNGILVDAAVTDANTAWNPQV
                     AFGAYVGAQQTSFTGSMDEIAVFNQALTPLQIQQIYNAFSAGICKPTLQTIAVNPPSP
                     TLVPGLSLQLDAVGSYSDNSTHDLTTSAIWGSSNSSVATVSPSGLVTANASGGTSVSA
                     SLGTTQGSTSLSVSSNLLSLQVNPQSPSTDIGVVTPFTATGTFSGGNQQDLTSSVNWM
                     SSAPAVATVGPSGQVTSLSAGQATITAIAGSVNASSVISVSSVALQSIAVSPANPQIA
                     TGTTQPFTAIGTFSDGTQQNLTASVSWSSSTPAATIAANGVSTGVGAGQTTITASMGS
                     VSGTASLTVTTAVLAAIQISPTSPSLAVGGSQQFSATAIYSDGSAANITASATWNSSS
                     TAVVSMSSTSPGLAAGMGSGQTTIAASFGGLTDSTTLSVQDPLVAISIAPSTTSVGAG
                     QTRQFIAIGT"
BASE COUNT          922 a         1457 c         1274 g          868 t
ORIGIN      
        1 ggctttgctc gcgactcggg acttgcaact cgggactgcc ttctagattg ccatcacgcc
       61 gacttctttt tccacttcca ccgtgtcgac catgacgatg gcgtcccagg ggcagccatc
      121 gaggaagcag ccgtcggggc ctttactcaa gcacttcttg cagccgatgc agaggatagg
      181 atcgaccgcg atgcggccga acggcggatg gtcttcgtcc ggcacccaga acatgcagtc
      241 ttcgaccggg caatattcca cgcaggcggg cgagccggcg catccggtgc agcattccgt
      301 gatcacggcc agctctttcg gtttcttctt gcgcggcgtg gcaaggcctt tggacttcgg
      361 ttcaaacttg agctcgtcgg gcaccatccc gacggccttg gtggctccgg gtgcggcagg
      421 catgagaggg attataccgt acctccggta gacccgaaac gacactccca ctctgcgttc
      481 gatcaatccc cacactatcc accggcttta ttctgctata gtttcggcaa cgaaggagct
      541 ctcccatggc tggaaaaagt ctgaaccggg ttcaactgat cggcaatctg ggcaaggatc
      601 ctgaaatcaa atacacgccg caaggcacgc ccgtggccaa gctggccatc gccaccaacg
      661 agcgcttcaa ggacaagagc ggtgaatggc aggaccgcac cgagtggcac aatgtcgtgc
      721 tctggcagcg actggcggaa attgccggcg agtatctgaa gaagggcggg aaggtctaca
      781 tcgaagggcg cttgcaaacc cgctcctggg atgacaaaca gaccggccag aagaaataca
      841 tgaccgaagt cgtcgccagc gacctgattc tgctcagcgg acgcggcgag ggaggcggtg
      901 gtggcgacta cagcagcagc tcgcgggccg cggctgcttc cggcggcaac aactttgacc
      961 agcgtgtgcc cgagcccgag cacgctaccg catccggtcc gatcacggac gaggacattc
     1021 ctttctgagc gcccctgagc gtggtaccgg ggatgcctca acgggccggc gcgccgcgcc
     1081 aggactcggt taccgtccgg ccttcgacgc gccttatagc ccgttggtaa tagcccgcaa
     1141 ctgcaatttc tttcacactg tccgcgacgc tgaaaggcgt gtatactcct gcctgtcgag
     1201 aggagcttcc ccgcaggaag ggcgttgatg aggttcacgc atgccaagct gacccccatt
     1261 gcgcacactt gtccgcgggt gactctggtt ctagtcctcc tttgttctgc cgtttccgcc
     1321 gcgcatgccc aaagcctgaa ttctacaagc gtctccttcg gcaacgtggt tcagaacacc
     1381 acgagcgctc ccaagaccgt tactcttact aactcgcagg cggttccact cgtcatcagc
     1441 agcattacgg tctcaggtga tttcgcggaa acgtcgaagt gcccgataag tccaaacaca
     1501 ctggctgcgg gcgcgacgtg ccagatccag gttacataca cgcctacagt gctggcgagc
     1561 gaaagcggca cgctggcggt caacgacaat gccacaaaca ccccgcagac cgcccaactg
     1621 agcggaaccg gggttccacc ggtcggcgtc acaccggcct cttatagttt cagcaaccag
     1681 ttcgtcggca ccaccagcgc ggcgcgcgtc gttgcggtca acaactatca gacaacgcca
     1741 ttgaccatcg ccagcatcgc cgccaccggc gatttcaccg tgaccacaac aacgtgccct
     1801 ctcgcgccaa acacgctggc ggcaaagacc acttgcacgg tgtccgtggc gttcgctcct
     1861 attgcggccg gcacgcgcaa gggaacgctg acggtgacct acaacgcgtc aaacagcccg
     1921 caatccgtcg ctttgtccgg cacgggcgtg cttccggtca gcgtgactcc tggcagcgtc
     1981 acgttttccg gacagcaccc cggcaacacg agcaacccta cgaatatcac cctgaaaaat
     2041 ggccagtcca caccgctgac gatctcggcg atttctacgt cgggagactt cgcggaatcg
     2101 tccactacct gcccactgtc tccgaatacg ttggcctcgg cagcgagctg tacgatctct
     2161 gtaacgttca ccccgactac ccttggcacc agaaccggaa gcctgaccat taccgacact
     2221 gccagcacca gcccgcagat tgtcaccctg tcaggaagcg gcgtgctgac cggcctgaat
     2281 acgatcactg tcacgccgag caatccatcg atgtttgtgg gcaatcagca acaattcacc
     2341 gcgaccgcca atttttcgaa tggctcaacg ctcaacatca ccagttgggt cacttggttc
     2401 acctcggatt ccgcgcaggc ccaggtaact tccggcggac tcgtgacagc cctggctccc
     2461 ggaaaagtgc tgatcgtagc gaagtatttg caaagttcgg gcagcactac ggccaatgtt
     2521 tccgcgaaaa atctgacctc gattacggtg actccgggca gctcatccct gccagttggt
     2581 gcgtatcaac agttcagtgc cttgctcaca tacagcgacg gaagcaccca gaatacgacc
     2641 agtgacgtcg cgtggtcgtc gtcttcaaag gcggtggcct cgatcaacgc taccggaatg
     2701 gccaacgcgt cctcgatcgg gagcacaacc atcaaggcca ccctcggatc ggtttcgggc
     2761 agcaccacgt ttacggtttt ccagccgtcg tgcgccgcgg ccccggcggg tctggtgggg
     2821 tggtggacgg gcgacggcaa tacggtcgac ctgggcggcc agaactccgg aacgttgcag
     2881 aacggggcaa gctatggcgc gggtgaagtc ggccaagcct tctcgttcac cgggaatggc
     2941 gcgtcggtac tcgtcaattc tccgctttat tcgcctccgg ccggaactct ttcgttctgg
     3001 ttcatgccaa cgggcagcgg agccctgacc ggcggctacg cgggaggaca gaaccgcgct
     3061 cccggcttgc tgatcgactc atccaacaac ctgaactggg agtatggcaa cctgtcgagc
     3121 cagtccatcg gtcaggtgaa cttaaatcag tggaatcatg cggccctgac ctattcgacg
     3181 tcgaattccc agacatccgt cagtgtctac ctgaatggca tactggtcga cgccgccgtg
     3241 accgacgcga atacggcctg gaacccgcaa gtcgcattcg gcgcgtacgt gggtgcacag
     3301 cagacatcat tcactggttc gatggatgaa atcgccgtct tcaaccaggc gctcacgcca
     3361 ttgcaaatcc agcagatcta caatgcgttt agcgccggca tttgcaagcc gacgctgcag
     3421 acgatcgccg tcaatccgcc ctcgccaaca cttgttcccg gactctcgct gcagcttgat
     3481 gccgtcggat cctatagtga caactcgacg cacgacctca ccacttcggc gatctggggc
     3541 tcaagcaact catcggtggc gactgtcagc ccgagcggct tggtcaccgc caacgcgagc
     3601 ggaggcactt ctgtttcggc ttctctcggc actacccagg gttcgaccag cttgtcggtt
     3661 tcatccaacc tgctctctct tcaggtgaac ccgcaaagtc cgtcgacgga catcggagtg
     3721 gtcactccgt tcacggcaac aggaactttc agcggcggca atcagcagga tctgacttcg
     3781 tctgtgaact ggatgagctc tgcacctgcg gtcgctactg ttggaccgag cggccaggtc
     3841 accagcctgt cggcaggtca ggccacgatc acggccatcg cgggatcggt gaacgcatct
     3901 tcagtgatta gcgtgagctc agtagcgctg cagtccatcg ctgtcagccc cgcaaatccc
     3961 cagattgcca caggcacaac ccagccattt accgcgatcg gtactttcag cgacggcacg
     4021 cagcagaatc taactgcgtc ggtgagctgg agctcatcaa ctccggcggc gacgatcgcg
     4081 gcaaacggcg tgtcgactgg cgtgggcgct ggccagacca caatcactgc gagcatggga
     4141 tcggtatcgg gtaccgcgag cctgacagtc acgaccgcag tgctggccgc aattcagatc
     4201 agtcctacca gcccctcgct ggcggtcggc ggatcgcaac agttctccgc gacggcgatc
     4261 tacagcgatg gcagtgccgc caacatcact gcaagcgcaa cctggaattc ctcctcgaca
     4321 gcagtcgtct ccatgagcag cacttctccc ggactcgccg ccggcatggg ttccgggcag
     4381 acgacgattg cggcgagctt tggcgggctc acggattcca ctactttgtc tgtccaggac
     4441 ccgctggttg cgatttcgat tgcgccctcg actacgtcgg tgggggcggg acaaacccgg
     4501 cagttcatcg cgatcggtac c
//
LOCUS       DASPUL010000654         4181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_677681, whole genome shotgun sequence.
ACCESSION   DASPUL010000654 DASPUL010000000
VERSION     DASPUL010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677681"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1948
                     /locus_tag="VEJ00_15070"
     CDS             <1..1948
                     /locus_tag="VEJ00_15070"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXX02535.1"
                     /translation="GSTADLTSLATWTAGSNAVSVSSAGMLKAMATGSSQITAQYQSI
                     TGNASVTVTKAALASIAVTPNPSSLPVGLSEQLSATGTFTDGSTMDLTQAASWSAAPS
                     TSVALNATGLLKAVATGSVQVSAQSQGVTGSAMVTVMPPALVSIAVTPGQSDVPVGRT
                     QQLTATGTYTDGSTADITTLATWSSNPSALAPVNAVGLVSGVAIGNVLVSASYQGKVG
                     QAAVTVPNLIFITISPNPSSLPKGTTEQLTATGLYNDGSKFDLSQSALWATSPSSTAS
                     IDTTGLVKGMGVGTAQVSAQYQGVTGNASLTVAPPIVVSINVTPNWSSVAVGKSEQLH
                     ATGTFSDGSTSDLTLTALWASTESAVASVSVHGSTTGSAAGVTPITASSGGVRGAAVV
                     TVLSAPIASLSVVPNSSTMGLGGSQQFLAIARLQNGVTQDVTDSVLWSSLPTNIVSLS
                     TGGFATATQVGATTVSASAGGMTGSAAVRVGPGMDVNYFDLANAQVSGADGTIRITNP
                     GTTGGDLCAMFYVFDENQELTECCGCSVSQNGLRTLSLTSDFNSNPLTGTPPARGVIQ
                     MVPADLSSNPTCNPASITPKGVILGWGSNIQVLNNSTFQFTETKFTPVPLSRAEQQLE
                     LVSQCAFLQQLGSGHGICSCGTGD"
     gene            2019..2186
                     /locus_tag="VEJ00_15075"
     CDS             2019..2186
                     /locus_tag="VEJ00_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02536.1"
                     /translation="MKDPCQILREKELDLERVRQQIEALHFVIPLLAEDSDWIEHGMA
                     PPYAQERAGRI"
     gene            complement(2211..>4181)
                     /locus_tag="VEJ00_15080"
     CDS             complement(2211..>4181)
                     /locus_tag="VEJ00_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02537.1"
                     /translation="KLWTGDTYVDFDLSLNSAVKKLRQALNDDSENPRFVETLYRRGY
                     RFIGPVNGLANGTGQIALVESLAHAAPPTSANQTTFSPPAIKNRLVAYRAVALLLAVV
                     AAGLYLLIPSQPPRVLGFTQITHDGLMKLGMVSDGERLYLLELQGDRFALGQVSVAGG
                     ETSIVPAPFPNVMLTGIAPNGSALIVGGFEGTGKGLGTWSLPLPSGPPRRIDNFRSDS
                     LDWSRDGSLAAFSVGPDIFLANHDLSEPRKIATAAGQVLGLRFSPDGRRLRFDLVDPG
                     NSSRSLWEVARNGSGLRQLLPGWNTVPRECCGNWTPDGKYYLFGSFRDGRESIWALPE
                     KSFWLSRTPQPVQLTNGPLDFQFPLVSKDGKRIFVIGSQPRCELVRYGGEAGFEPYFD
                     GKSIRDLTFSADGKWMAYVSVPEAQLWRSRIDGSERLQLTPDGMNAGLPRWSPDGKQI
                     VFMGTDFKTDWLAYLVSSDGTGLRKMNPGIDSAYDPGWSADGKSIVFTLEPGGDPSSR
                     LQKGIAIDDLGTGKISPLPDATHLFSPRWSPDGRYIAAITNDSLKLMLFDRVSQQWQD
                     LVNLVNLPIGYPSWSHDSQYIYFDTTLNEDPAFFRIRISDHKLERLFSLSGMRRYWGP
                     FGPWTGLAPDDTPLLVRDISNQEIYALDWQAQ"
BASE COUNT          861 a         1283 c         1254 g          783 t
ORIGIN      
        1 cggatcgacc gcggatctga cgtctttggc gacgtggacc gcaggctcga atgcggtcag
       61 cgtaagttcg gcgggaatgc tgaaagcgat ggcaaccgga agctcgcaga ttacagccca
      121 gtatcagagc ataacgggca atgcctcagt cacagtcaca aaggcagcac tggcctcgat
      181 cgcagtgact ccgaatccgt cttccttgcc ggtaggccta tccgaacaac tgtccgccac
      241 cggaacgttc accgacggct ccacaatgga cctcacgcag gccgcgagct ggagtgccgc
      301 tccgtccacc tcagtcgctc tcaatgcgac aggcttgctg aaggcagtgg cgacggggag
      361 cgtacaggtc tcggcgcaat ctcaaggcgt cactggcagt gccatggtga cggtcatgcc
      421 gcccgctctg gtctcgatcg cggtgacccc cggccagtca gatgtgccgg ttggccgcac
      481 ccagcaactc accgccaccg gcacgtacac cgacggatcc acggcggaca ttacaaccct
      541 ggccacgtgg agctcgaacc catccgcgct ggctccggtc aacgccgtgg gcctggtgtc
      601 cggtgtcgcg atcggaaacg tgctggtctc cgccagctac caggggaaag ttggtcaggc
      661 cgcagtcacg gtacccaacc tgatcttcat cacgatcagc ccgaatccgt cctctttgcc
      721 gaagggcacg accgaacagt tgacggcgac cggcctctat aacgacggat ccaagttcga
      781 tctcagccag tcggctttgt gggcgacgag cccctcgagc accgcgtcca tcgataccac
      841 cggtttagta aaaggaatgg gcgtgggcac ggcgcaggtc agcgcacagt accagggcgt
      901 cacagggaac gcctcgctca cggttgcacc gccaatcgtg gtatccatca acgttactcc
      961 aaactggtct tctgtggcgg tgggcaaatc agagcaactg catgccaccg ggacgttcag
     1021 cgatgggtcg acctccgacc tgacgctgac ggccctgtgg gcctccacag agagtgcggt
     1081 cgcgagcgtc agcgtgcacg gatcaacgac cggcagcgcg gcgggcgtga cgccgattac
     1141 agcgtcttcc ggcggcgtca ggggcgcagc tgtggtgacc gtgctgtccg cgccgatcgc
     1201 gtccttgagt gtggttccta actcgtccac gatggggctg ggcggaagcc agcaattcct
     1261 ggctatcgcc cggttgcaaa acggagtgac acaagacgtc acagacagcg tgctctggag
     1321 ttccttgccg acgaacatcg tcagtttgag cacgggtggg tttgcgaccg caactcaggt
     1381 gggcgcaacc acggtgtctg ccagcgccgg cggtatgacg ggttcggcag ctgtcagggt
     1441 cggccccggg atggacgtca actacttcga cctggccaac gcacaggttt cgggagcaga
     1501 cgggactatt cgcatcacca accccggcac cacaggcggc gatctctgcg ccatgttcta
     1561 cgtgttcgac gagaaccagg aactgaccga gtgctgcggt tgcagcgtgt cgcaaaacgg
     1621 cctgcgtacg ctgtcactga cctccgattt caactccaat cctctgaccg gcactccgcc
     1681 ggcacgggga gttatccaga tggtgcctgc tgatctctcg tcgaatccga cctgcaaccc
     1741 tgcctcaatc acgccgaaag gagtgatcct cggttggggg agcaatatcc aggtgctcaa
     1801 caacagcacg ttccagttca ccgagacaaa atttaccccc gtcccgctga gccgcgcgga
     1861 acagcaactg gaactggtca gccagtgcgc cttcctgcag caactgggca gtggacatgg
     1921 aatctgcagt tgcgggactg gcgactagac gggaacataa gcccgttatg gcgtttgcgg
     1981 aggcagcgag cgatcacggc gaaccaagga gtcgaatcat gaaggatccg tgccagattc
     2041 tcagagagaa agagctggac ctcgagcgcg ttcgccaaca aatcgaagcg ctgcattttg
     2101 tcatcccgtt gctcgccgaa gattccgact ggatcgagca cgggatggcc ccgccgtacg
     2161 cccaggagcg cgccggacgt atctagctaa ccagcccgtg cgcatgggag ttactgcgcc
     2221 tgccagtcga gggcgtagat ttcctggttg ctgatgtcgc ggaccaacaa gggcgtatca
     2281 tctggcgcca aacccgtcca tgggccgaac ggtccccagt accgccgcat cccactcagg
     2341 ctgaaaagcc tttccagctt gtgatccgat atccgtatcc gaaagaaggc ggggtcttcg
     2401 tttaaggtag tgtcaaagta aatgtactga ctgtcgtgcg accaactggg atagccgatc
     2461 ggcaggttca ccaggttcac caagtcttgc cattgctggc tgacccgatc gaacagcatc
     2521 agcttgagcg agtcgttcgt aatagcggcg atgtagcgtc cgtccgggga ccagcgtggg
     2581 ctgaacaaat gggtcgcgtc gggaagggga gagatctttc cggttcccag atcgtcaatg
     2641 gcaatgccct tctggaggcg ggagctcggg tcacctccgg gctccagcgt aaacacgatg
     2701 gactttccgt ccgcggacca gccaggatca taggcactgt caatgccagg gttcattttc
     2761 cgcaagcctg taccgtcgct ggaaaccaga taggccagcc agtcggtctt gaagtccgtg
     2821 cccataaaca cgatctgttt tccgtccggt gaccagcgcg gcagaccggc attcattcca
     2881 tcgggcgtaa gttggagcct ttcgctgccg tcaatccggc tccgccagag ctgagcttcc
     2941 ggcaccgaca catatgccat ccactttccg tcggcagaaa acgtcaggtc ccggatcgac
     3001 ttgccgtcaa aatatggttc aaaaccggcc tcgccgccgt aacgtacaag ttcgcatcgc
     3061 ggctgcgagc cgatcacaaa aatccgtttc ccatccttgc tcaccaaagg aaattgaaaa
     3121 tccagcgggc cgttggttag ctgtacgggc tgtggagtcc ggctaagcca gaaggacttt
     3181 tctggcagtg cccatatgct ctccctgcca tcgcggaaac ttccaaacag gtaatacttg
     3241 ccgtcgggag tccagttccc gcagcactcg cggggaacgg tgttccagcc cggtagtagt
     3301 tggcgcaggc cacttccgtt gcgggcgact tcccagagcg agcgcgagct attgccggga
     3361 tccaccaggt cgaagcgaag cctgcgaccg tccggagaaa accgcagccc cagaacctgc
     3421 cccgcggctg tggcgatctt gcgtggttcg ctcaggtcgt ggtttgcgag gaagatgtcc
     3481 ggccccacgg agaacgcggc gaggcttcca tctcgcgacc agtccagcga atccgatcgg
     3541 aagttgtcga tccgccgcgg aggtccgctg ggcaggggta gagaccacgt tccgagcccc
     3601 ttacctgttc cttcaaagcc gcctacgatc agagctgatc cgttcggagc gatgccggtg
     3661 agcatgacat tggggaaggg cgcaggcacg atgctggttt cgccccccgc gaccgatacc
     3721 tgacccaagg cgaagcgatc gccctgcagt tcgagaaggt agagccgctc accatcgcta
     3781 accatgccga gcttcatgag cccgtcgtgg gtgatttggg taaatcccag aactcgcggc
     3841 ggctgggaag gaatcaacag gtatagacct gcggccacca ccgccagaag caacgcgacg
     3901 gcacgataag caacaaggcg gttttttatg gccggaggcg agaacgtcgt ctggttcgcc
     3961 gacgtgggtg gagctgcgtg tgcaaggctc tcgaccagcg caatctgacc ggtgccgttc
     4021 gccaatccgt ttaccggccc gatgaatcga taaccccggc gatacagcgt ttcgacgaat
     4081 cgcggattct cggaatcgtc attcagagcc tgccggagtt tcttgaccgc actgttcagg
     4141 ctcaagtcga agtctacgta ggtgtcgccc gtccaaagct t
//
LOCUS       DASPUL010000655         5960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1627551, whole genome shotgun sequence.
ACCESSION   DASPUL010000655 DASPUL010000000
VERSION     DASPUL010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627551"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VEJ00_15085"
     CDS             complement(<1..250)
                     /locus_tag="VEJ00_15085"
                     /inference="COORDINATES: protein motif:HMM:NF026042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02538.1"
                     /translation="MSEWAQFEAIEGADYWIKMVKCQHACPVHTNACGYVTAIGDGRY
                     EEAYRIARATNPFASICGRVCGAPCEVNCRRGDVDAPVS"
     gene            complement(263..607)
                     /locus_tag="VEJ00_15090"
     CDS             complement(263..607)
                     /locus_tag="VEJ00_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02539.1"
                     /translation="MAEIKVPEAKITDEPKKTSDTEIQEYADGWITERKGTDVPPFLK
                     LAYIVIIAGVIGYFLLYMNGEVNHSDRGALVRQFNQFTQSSPVLMYSVIAMVVIYALI
                     VVAFAFKAVHKD"
     gene            complement(600..815)
                     /locus_tag="VEJ00_15095"
     CDS             complement(600..815)
                     /locus_tag="VEJ00_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02540.1"
                     /translation="MHDYVYPSVFLASLLFFLCLAGAIFFLVRSRRDGYWGKESEEPK
                     YRMLADDETGGSPGDGSQTDLKEARHG"
     gene            complement(824..1393)
                     /locus_tag="VEJ00_15100"
     CDS             complement(824..1393)
                     /locus_tag="VEJ00_15100"
                     /inference="COORDINATES: protein motif:HMM:NF014486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HXX02541.1"
                     /translation="MLGKTDKSAIGFVIAALVFFFIGGLLTTVVPPLVDKSWSKPFEN
                     DDPSKGPTGKLRPYTELELKGRAIYVREGCWYCHTQQTRTLLADTKRSGWKGVDSPIS
                     TPDEFVYDSPHMFGTKRTGPDLSRVGGKYDRQWHRTHFRNPRDLVPGSVMPPFPWIVN
                     NDEEFNALVAYIQTLGRAKNWRPDNDYEK"
     gene            complement(1404..2840)
                     /locus_tag="VEJ00_15105"
     CDS             complement(1404..2840)
                     /locus_tag="VEJ00_15105"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXX02542.1"
                     /translation="MPEQGQRQLQVTRKTPIGAVPLADSIHEDTTAKWFLLSAISYFF
                     VVGIIALTIAAKFVWPQLLGTVPYLSYGRLRPLHVNGMLFGWLLPADLGLAYYVVPRL
                     CGVKLWSEKLGIATAVLWNVIVLGAVYALPAGYNKGWEYAELPLPLVALVVVAWVMFG
                     LNIFATVATRRYPQMYVSLWYTMATVLWTTFVYPTGNVAVLFTTGTNQANLNWMYVHN
                     AVGLIFTPIGLAMAYYFIPKASNTPLYSHKLSMIGFWSLAFVYVWTGAHHMIHGPISQ
                     WLQTIAIAFSLMLLIPVWTVVYNFFATMKGQWHQLRDNIPLKFLMSGVVFYLLTCFQG
                     PMHSLRSVNAIVSKTDWIPGHAHMALLGCFSFFAIAGAYYVVPRMFHTKLHSDALANW
                     TFWFFMIGGLGFFVTLWLGGFWQGWQWTNPSIPFIDTVVALKPVWTVRFFSGVLMFAG
                     IATFLFNMLATVVGRGQATAQTAPAFTD"
     gene            complement(2807..4693)
                     /locus_tag="VEJ00_15110"
     CDS             complement(2807..4693)
                     /locus_tag="VEJ00_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXX02543.1"
                     /translation="MSTRVTLAVVSFVVLIVHGIVFYDQFFNRWEKHQTAYFEQARSL
                     AKTDAERRALDERSPRIEQIIVTQFGESRVDRCTTCHIASDDPRFQGHAEPLRTHPYS
                     AALGDVQHDGRWERRHKFTDFGCSICHDGQGRGLETFYAHGEDIFWPDPMLGYVTQAN
                     WHSQYKPKLTGKEYMQANCAQCHTEENFAGTPLVQRGRQLFFEKACYRCHRIEGLSTG
                     TLGPDLSEVGKKYKINYLWEHTVDPRAFQVTSFMPKFDLTDDEIKALTVFLKSRRGIN
                     FAETSLGQYRARLQQTKLEEATKPQSLAAPSPVRGEQLVSERSCTACHKLGERDGHIA
                     PDLSYEGLIRDEDWMMAHFHEPKSRIPDSIMPVFGFAEADFHSITAYLKTRSAPPAFK
                     TGEEIYKGLCARCHGEKGDGKGVTYLYLDPAPRDLTKAAFMNSKPEDRFLQSLKNGVP
                     GTSMPPWERVLTEEQRRAVLAYVFQTFVKEPRPELKVRKVPDQNPVASSADSIHRGEQ
                     TFLQRCTGCHGRKADGKGPNSLDISPRPRNLRNSFFVNSVPDRRLFDSILYGVQGTAM
                     PSWIDYGLSQNDVGDLINFIRSMNSRTGSSPGEKSAALTPQVNLSQMNQEVPCLNKGK
                     DNCR"
     gene            complement(4727..>5960)
                     /locus_tag="VEJ00_15115"
     CDS             complement(4727..>5960)
                     /locus_tag="VEJ00_15115"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HXX02544.1"
                     /translation="DLGRASTSFTNFFLHLHPVKVHRHSLKATYTLGLGLISFFLFFI
                     LVVTGILLMFYYVPSTTQAYDRMLDLRGTVAFGIFLRNMHRWSAHGMVAVVFLHMCRV
                     FLTGSYKPPREFNWVLGVLLLLVTLFLSFTGYLLPWDQLAFWAITVGTSIASYAPLVG
                     KQMKFILLGDSNVGQEALLRFYVLHVAVLPAILSLLVAIHFWRIRKDGGLSRPEKEDA
                     LPADAPAQVEVMQGPAVVMATSPIAAAAAEPVLQKRHYGIQGLVRGPFTKIGNVPDRS
                     VFAWPNLFMAELVVFLITVAGVLLVSLFFNAPLEEPVNVMHPPNPAKAPWYFLGLQEQ
                     VSYSAFWGGVGIPATEVLLLLLVPYIDRKVAGVGRWFARERLLANFLFLTFVIVNVVL
                     IIIGTFFRGPNWEFVSPW"
BASE COUNT         1261 a         1701 c         1869 g         1129 t
ORIGIN      
        1 tggacacggg cgcgtcaacg tcgccgcggc ggcaattcac ttcacacgga gcgccacaca
       61 cccggccgca gatggacgcg aacggattgg tggcgcgggc gatacggtaa gcctcttcat
      121 agcggccatc gccgattgcc gtcacgtagc cgcatgcgtt cgtgtggacg ggacaggcgt
      181 gctggcactt gaccatcttg atccagtagt cagccccttc aatcgcttcg aactgtgccc
      241 attcgctcat cggtctgcgc cgttagtcct tgtgaacggc tttgaaggca aaggcaacca
      301 cgatgagcgc atatatcacc accatcgcaa tcacggaata catcagtacc ggcgaagact
      361 gggtgaactg gttaaactgg cggaccagcg cgcctcggtc ggaatgattg acttccccgt
      421 tcatgtagag aaggaagtag ccgatgacac cggcgatgat gacgatgtac gccagtttca
      481 ggaatggcgg cacgtcggtg cctttgcgtt cggtgatcca gccgtcggcg tattcctgga
      541 tctcggtgtc cgatgtcttt ttgggctcgt ctgtaatttt ggcctctgga actttgatct
      601 cagccatgtc ttgcctcctt caaatctgtc tgggagccgt caccggggct gccgccggtt
      661 tcgtcgtcgg ccagcatgcg atacttcggc tcctcacttt cctttcccca atacccgtcg
      721 cggcgggagc gcacgagaaa gaagatggct cccgcaaggc acagaaagaa cagcagggac
      781 gctagaaaaa cgctggggta aacgtagtcg tgcatcgcga cctctatttc tcgtagtcgt
      841 tgtccggccg ccagttcttg gcgcggccga gagtctggat ataggccacg agagcgttga
      901 actcctcgtc attgttgaca atccacggga acggcggcat caccgagccg ggaaccaggt
      961 cacggggatt gcggaaatgg gtgcggtgcc actgtcggtc atacttgccg ccgacacggg
     1021 aaagatcggg cccggtacgt ttggtgccga acatgtgcgg actgtcgtag acgaactcgt
     1081 caggtgtcga gatgggcgag tcgacgcctt tccatcccga acgcttggtg tcggccagca
     1141 gggtgcgcgt ctgctgcgtg tggcaatacc agcagccttc gcgcacgtag atggcgcggc
     1201 ccttgagttc gagttccgtg taggggcgca gcttgccggt cggccccttg gaagggtcgt
     1261 cgttctcgaa cggcttggac cagctcttgt cgaccagcgg cgggacgacc gtggtcagca
     1321 atcctccgat gaagaagaac accaaagcag cgatgacgaa tccgattgcg cttttgtcag
     1381 tttttcctag catggcgttc tccttagtcg gtgaaagctg gcgccgtttg cgccgttgcc
     1441 tggccgcggc ccacgaccgt agccagcatg ttgaacagga aggttgcaat gccggcgaac
     1501 atcagcacgc ccgagaagaa gcgcacggtc cacaccggtt tcagcgcaac cacagtgtcg
     1561 atgaacggga tcgacgggtt cgtccactgc cagccctgcc agaatccgcc cagccagagc
     1621 gtcacgaaga atccgaggcc gccgatcatg aaaaaccaga aggtccagtt ggcgagggcg
     1681 tcggagtgca gtttggtgtg gaacattcgc ggaaccacgt agtaggcgcc ggcgatcgcg
     1741 aaaaacgaga agcacccgag caatgccatg tgcgcgtggc cgggaatcca gtcggttttc
     1801 gagacgatgg cattcacgga ccgcaggctg tgcatggggc cctggaagca ggtcagcagg
     1861 taaaacacga ctcccgacat gaggaacttc aacggaatgt tgtcgcgcag ttggtgccac
     1921 tgccctttca tcgtggcgaa gaagttgtag accaccgtcc acaccgggat cagcagcatg
     1981 agagagaacg cgatggcgat ggtctgcagc cactgcgaga tcggcccatg aatcatgtgg
     2041 tgggcacccg tccagacata gacgaaagcc agcgaccaga agccgatcat cgatagtttg
     2101 tggctgtaca gcggcgtgtt cgaggccttg gggatgaagt agtaggccat ggccaggccg
     2161 atcggggtaa agatcagccc gaccgcgttg tgcacgtaca tccagttgag gttggcctga
     2221 ttggtccccg tcgtgaacaa caccgcgacg ttgccggtgg ggtaaacgaa cgtggtccaa
     2281 aggaccgtag ccatcgtgta ccacaggctg acgtacatct gcggatagcg gcgagtggcg
     2341 acggtggcga agatgttgag gccgaacatc acccaggcca cgacgaccag agccacaagc
     2401 ggcaggggca gctccgcgta ttcccagcct ttgttgtagc ctgccggcag agcgtagacg
     2461 gcgccgagaa caatcacgtt ccagagaacg gcggtcgcga tgccgagctt ttcactccac
     2521 agctttactc cgcagaggcg aggcacaacg tagtaggcaa gtccgagatc ggccggaagc
     2581 agccagccga acagcatccc gttgacgtgc agcggccgaa gccggccata ggaaaggtag
     2641 gggaccgttc ccagcaactg cggccagacg aacttggccg caatggtcaa agcgatgatt
     2701 cccacaacaa agaaataaga gatggcactc agcaaaaacc actttgccgt cgtgtcttcg
     2761 tggatcgaat cagccagtgg aactgcgccg attggggtct tgcgcgttac ctgcaattgt
     2821 ctttgccctt gttcaggcat ggcacctcct gattcatttg ggacaggttc acttggggag
     2881 tgagcgctgc ggatttctca ccgggagatg agcctgtccg ggaattcata ctgcgaatga
     2941 agttgatgag atcgcccacg tcgttctgac tcaggccata gtcgatccat gaaggcatgg
     3001 ccgtgccctg cacgccgtac aggatggaat cgaagagcct gcggtccggc acgctgttca
     3061 caaagaagga attgcgcagg ttgcgcggcc gcgggctgat gtcgagcgag ttcggtccct
     3121 tgccgtcggc cttacgcccg tggcaacccg tgcaacgctg gaggaaggtt tgttctccgc
     3181 ggtggatcga gtcggcgctg gaggcaaccg gattctgatc cggtaccttg cgcaccttga
     3241 gttcgggacg cggctctttc acaaacgtct ggaaaacgta agccaggacc gcgcggcgct
     3301 gctcttcggt cagaacgcgc tcccacggcg gcatcgaggt cccgggaacg ccgtttttca
     3361 gggattgcag gaagcggtct tcgggcttgc tgttcatgaa ggcggccttg gtcaggtcgc
     3421 gcggcgcggg atcgagatag aggtaagtca cgcccttgcc gtcgcctttc tcgccgtggc
     3481 agcgggcaca aagccccttg tagatttctt ctccggtctt gaacgcaggc ggcgcgctgc
     3541 gcgtcttcag ataggcagta atggagtgga agtcggcctc ggcaaaccca aataccggca
     3601 tgatcgagtc gggaatgcgc gacttcggct cgtggaagtg ggccatcatc cagtcttcat
     3661 cgcggatcag tccttcgtag gaaagatcgg gggcgatatg gccgtcgcgc tcgcccagct
     3721 tgtggcaggc ggtgcaggaa cgttccgaga cgagttgttc gccgcgaacc ggcgaaggcg
     3781 cagcaaggct ctgcggcttg gttgcctctt ccagtttcgt ctgctgcagc cgggcacgat
     3841 actggccgag ggaagtctcg gcgaaattga ttccgcgacg gctcttcagg aagacggtga
     3901 gcgccttgat ctcgtcgtcg gtgagatcga acttcggcat gaacgaagtc acctggaacg
     3961 cgcggggatc gaccgtgtgc tcccacaggt agttgatctt gtatttcttg ccgacttcgc
     4021 tgagatcggg ccccaaggtt ccggtggaga ggccctcgat gcgatggcag cggtagcagg
     4081 ctttttcgaa gaacagctgc cttccgcgct gcaccagcgg tgtgccggcg aaattctctt
     4141 cggtgtggca ctgcgcgcag ttggcctgca tgtactcctt gccggtgagt ttcggcttgt
     4201 attgggagtg ccagttggcc tgggtcacgt aacccaacat gggatcgggc cagaagatgt
     4261 cttccccgtg cgcgtagaag gtctcgagcc cgcggccttg cccgtcgtgg caaatgctgc
     4321 aaccgaagtc ggtgaacttg tgccggcgct cccagcgccc gtcgtgctgc acatcgccca
     4381 gcgctgccga ataagggtgc gttcgcagcg gttcggcatg tccctgaaag cgcggatcgt
     4441 cgctggcgat gtggcaggta gtgcagcggt cgacgcggga ttcgccgaat tgcgtgacga
     4501 tgatctgctc gatgcgcggg ctgcgctcgt cgagggcgcg ccgttcagcg tcggtcttgg
     4561 cgagactgcg ggcctgctcg aagtaggcgg tctggtgctt ctcccagcga ttgaagaact
     4621 gatcgtagaa gacaatgccg tgcacgatca gcaccacaaa actcacgaca gccagcgtta
     4681 cgcgcgtact cacaaagttc tcctcacgtt ccggtcggct ttttcctcac cagggcgaaa
     4741 cgaactccca gttcggacct cgaaagaacg tgccgatgat gatcaagaca acgttcacga
     4801 tcacgaatgt cagaaacaga aaattggcca gcaggcgctc gcgggcaaac cagcggccca
     4861 cgccggcgac cttgcggtcg atgtagggaa ccagcagcag aaggagcact tcggtcgccg
     4921 gaatcccgac tccgccccag aatgcggagt aactgacctg ctcctgcagg ccgaggaaat
     4981 accacggagc cttggccggg ttgggaggat gcatgacgtt caccggctct tccagcggcg
     5041 cattgaagaa cagcgaaacc agcaacacac cggctaccgt gatcagaaaa acgacgagct
     5101 cggccatgaa gagattgggc caggcgaaca ccgagcgatc cggaacattg ccgattttcg
     5161 tgaacggccc acgcaccagc ccctggatgc cgtagtgccg cttctgcagt accggctcgg
     5221 ccgcagccgc agcaatagga gacgtggcca ttaccactgc cgggccttgc atgacttcaa
     5281 cctgagcggg agcatctgcg ggaagcgcat cttctttttc cgggcgcgac aagccgccat
     5341 ccttgcgaat tcgccagaag tgaatcgcca cgagcagcga gaggatggca ggcagcaccg
     5401 cgacgtggag cacatagaag cgcaacagcg cttcctggcc gacgtttgag tctcccagca
     5461 ggatgaactt catctgcttg ccgaccaggg gcgcgtaact ggcgatcgat gttccaaccg
     5521 tgatggccca gaacgcgagc tgatcccaag gcaggagata gccggtgaag ctcagaaaca
     5581 gcgtgaccag cagcaaaaga actcccagca cccagttgaa ctcccgcgga ggcttgtagg
     5641 aaccggtcag gaacacgcgg cacatgtgca ggaagaccac cgcgaccatg ccgtgcgccg
     5701 accagcgatg catgttgcgc agaaaaatgc cgaacgcgac cgtgccccgc agatcgagca
     5761 tgcggtcata ggcctgcgtg gtggagggca cgtagtagaa catcagcaaa atgccggtca
     5821 cgaccagaat gaagaagaga aagaacgaaa tcagccccaa acccagcgtg taggtggctt
     5881 tcaggctgtg gcggtgcacc ttgaccggat gaaggtgcag gaagaaatta gtgaagctag
     5941 tcgaagcccg ccccagatcg
//
LOCUS       DASPUL010000656         5047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_399017, whole genome shotgun sequence.
ACCESSION   DASPUL010000656 DASPUL010000000
VERSION     DASPUL010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399017"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VEJ00_15120"
     CDS             <1..251
                     /locus_tag="VEJ00_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02545.1"
                     /translation="ACYISDLIPTTAHIDLTWGMGFDLYPLDIIESKKQYYAQSIPEK
                     WLTVFTHDPTTPWAYVEKDESGKMVARGVPTDDVGVRK"
     gene            930..4685
                     /locus_tag="VEJ00_15125"
     CDS             930..4685
                     /locus_tag="VEJ00_15125"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXX02546.1"
                     /translation="MPKAMACRFGVLALVVAVFGVISAFSQATGSLRGTITDPTGAVI
                     PEASVTIKSADTGTVRKSLTDTQGEYTFQQVPPGTYKLVAEKAGFATMAKGDVTLLVN
                     TPATLDLKMSLSASGEIINVQSEASQVNTTDASVGNPFGEVQVRQLPLQTRNVVELLS
                     LQPGVTTDGEVMGGHRDQNNITLDGADVNNNQNSGLVAQATGTGTGGFQGSNANGSTI
                     NAGFNGVLPIPLDSVQEFRVTVAGEGPNLGRSSGGQVALVTKSGTNQLHGSLYEYNRN
                     TWFAANTWFNNHAGVPRQPLVRNQFGASVGGKIIPDRVFYFFNYEQREDASGVSQVRA
                     VPSDTLRKGIFQFKSGGATQTLQSSDITKVDPLNIGPDANILTVLQAYPSGNDPSYGE
                     DGGLNFSGYRFNAPFHRSDHALVGKIDVHVDRAGKHTVNVRTTFGRNTDDQIPAQFPH
                     QPAASTLRDNSKGVAAQYTAVLTPNLVNVVNLGYTRFGQSFSGQTGPLLFQTSLDPLL
                     NPYARPSAQVLPTTNLTDGITWVRGKHTLTAGGGFTLIRNNTTSYATSFARYGYGSTE
                     LIGLGADIDNAISQYTGLPLDDPTSAANGMGVVLGLVNDDFHTDLYNRTGAYLQAGTP
                     QVRSFIEHDYNLYAGDTFRVSRLLTLNFGLRYENFRPPYEAHGLQVTSTVPLTSYFAE
                     RNKLQAQGVPQNQMPNATLQWALNGPVNGKPTWWAPSNLNFAPRIGIAFAPASDEGLL
                     SKIFGKQGALRIGAAMVYDRFGSDLITQYDQYGSIGMATSGNFPTSYSFSTSPRYTGA
                     APVLPTPAAETFPVTPPNIAAIAGEFMGIAPNLKPPYSYVLNASFARELPGKLTFEVG
                     YAGRLSRRLLLEGDVYTPLEYFKDPKSGITWEQNAMALRKLHDSGVTVAQVLANPGIV
                     PTMPFVENMWPGLANLDVNGSASANYFTCVYNDYGGSYLDCLHAVDRNPSTAWGGTSN
                     LVGCAQISTYGCYTFFPLQGSSMPTWMNAGTAAFHALTFSLRRGLTSGLTFDFNYTWS
                     HSIDDGSAAESGAGQQGAAIQNIFNTREFRGSSDFDMRHNITANVLYPLPIGRGRMLL
                     VNAHGWLNEIVGGWAVSSLVRYHSGVPTTVGGELAYNANYWLSSLAIATAPVSAGIHT
                     DENGYPSMFSSTSISNNFADELPGHTGQRAVLRLPGMFVSDMAVTKNFPLPWEGQHLQ
                     FRAEAFNVFNNVNYSNPSLSLQAPGTFGEFQGVGDPRVMQFALRFEF"
BASE COUNT         1092 a         1550 c         1394 g         1011 t
ORIGIN      
        1 cggcctgcta catctccgat ctcatcccca ccaccgccca catcgacctc acctggggca
       61 tgggattcga tctgtatccc ctcgacatca tcgagagcaa gaagcagtac tacgcgcagt
      121 cgatcccgga gaagtggctg acggtgttca cgcacgatcc aacaacgccg tgggcatatg
      181 tggaaaagga tgagtcgggg aagatggtgg caagaggagt gccaaccgat gatgtaggtg
      241 tgcgcaaatg atgaaagtcc ggctcgagaa cgtgaaagtt cgaaccccaa agatgaactg
      301 gaaagtaagc tattggaacc aatgacatag gcggtcgtta gcccgctcga aatggacttc
      361 ccgcgcatag ccgaaccggg atcgaccggg tcgtataacg gccgatcagg catgttggac
      421 acgatcaccg catgaagcac gcttttccac tatcgagtga gcagaccgcg tgcctctcat
      481 tgcaatgtca gtcgttggat ggtgcggtgc ctttacccag cgacttgccg ggtagacgag
      541 tcattggagc aggcgtccaa tagaactaca cgccatcgac attgatctcg gcaagacagg
      601 ctttcatctc gttgggcttg acccgcgcgg aaagatcgtt ctacggaaga agttatttgc
      661 atacgagcaa gaccagctat cgaagcgggc aattcgacgc atcgatttat tgcctttgac
      721 agctctctcc aaatagctca cattcgcagg acagctggtt caggtctgca ttccctgtac
      781 ccaggtaaca ataggtttca aagttgacgg ctctgtaagt cttcgctggc caggacgcct
      841 tggcgaagaa atggtcttga cgccgcgcac ctcgaagtgt gcattgcgag accgtcgatc
      901 tcatttcttt gacattgggg aggaacacca tgcccaaggc aatggcctgc aggttcggag
      961 tgttagcgct ggtagtggct gtctttggag tgatcagcgc attctcgcaa gcaaccggtt
     1021 cactccgcgg aaccattacc gatccaaccg gcgcggtaat tcctgaggcg tcggtaacga
     1081 tcaagagcgc cgataccggc acggttcgca agagcctgac cgatactcaa ggtgaatata
     1141 ccttccagca ggttcctccc ggaacataca aattggtggc ggagaaggcg ggcttcgcga
     1201 cgatggcgaa gggcgatgtc acgctgctcg tgaacacgcc ggcaacgctc gacctcaaga
     1261 tgtcgctcag cgccagcggc gaaatcatca acgtacaatc tgaagcgtct caggtgaaca
     1321 ccaccgacgc cagtgtgggc aatccttttg gcgaagtaca ggtccggcag ctgccgctgc
     1381 agacgcgcaa tgtcgtcgaa ttgctcagcc tgcaacctgg agttacgacg gacggtgaag
     1441 tcatgggtgg ccaccgggat cagaacaaca tcacgctcga tggcgcggac gtaaacaaca
     1501 accagaactc cggactcgtc gctcaagcga caggcacggg aacaggcgga ttccaaggct
     1561 ccaacgcgaa tggctcaact atcaacgccg gtttcaacgg cgtgcttccg atcccgctgg
     1621 actctgtgca ggaattccgc gtgaccgtag ccggagaagg tccaaacctc ggacgttctt
     1681 ccggtggcca ggttgctctc gtgacgaaga gcggaaccaa ccagctccac ggctcactct
     1741 acgaatacaa ccgcaacacg tggttcgcag ccaacacttg gttcaataat catgcgggtg
     1801 tgccacgcca gcccttggtg cggaatcagt tcggcgcatc ggtgggaggc aaaatcattc
     1861 ccgatcgcgt cttctacttc ttcaactacg agcagcgcga agacgccagc ggagtttcgc
     1921 aggttcgcgc cgtcccatcg gacacgctac gcaagggcat cttccagttc aaatccggag
     1981 gtgctacgca aacgctgcaa tcctccgaca tcacgaaagt cgatcctcta aacatcggac
     2041 ccgacgcaaa cattctcacc gtgttgcagg catatccgag cggaaacgat ccatcctacg
     2101 gtgaggatgg cggcctgaac ttcagcggct accgtttcaa cgctcccttc catcgcagcg
     2161 accatgctct ggtcggaaag atcgatgtgc acgtcgatcg cgccggcaag cacacggtca
     2221 acgtacgcac cacgttcggt cgcaacacgg acgatcagat tccagcgcag ttcccgcatc
     2281 agccggcggc ttccacgctg cgcgacaaca gcaaaggtgt cgctgcgcaa tacacggctg
     2341 tcctgactcc caacctggtc aacgtggtca atctcggcta cacacgcttt gggcaatctt
     2401 tcagcggtca aaccggtccg ctgctgtttc agaccagtct ggatccgttg ctgaatccgt
     2461 atgcgcggcc ttcggcgcag gttctgccga ccacgaatct taccgacggg atcacctggg
     2521 tccgcggcaa gcacacgctc accgccggcg gcggattcac cctgatccgc aacaacacca
     2581 cgtcatacgc aacttcattc gcgcgctacg gctatggatc gaccgaactc attggcctcg
     2641 gcgccgacat cgacaatgcc atttctcaat acaccggctt accgctcgac gatcccactt
     2701 cggcggccaa cggcatgggc gtcgtactcg gtctggtaaa cgacgatttc cacacggatc
     2761 tctacaatcg caccggtgcg tacctgcaag ccggaactcc gcaggtgcgc tcgtttatcg
     2821 agcacgatta caacctgtac gccggggaca cgttccgcgt ctctcgcttg ctgacgctga
     2881 actttggcct gcgctatgag aatttccgtc cgccgtacga ggcgcacgga ttgcaggtca
     2941 cttcaaccgt tcccttgacc agctacttcg cggaacgaaa caagctgcaa gcgcaaggcg
     3001 tgccgcagaa tcagatgccc aacgccactc tgcagtgggc attgaacgga cctgtaaacg
     3061 ggaaaccaac ctggtgggct ccgtcgaatc tgaatttcgc gccgcgtatc ggtatcgcat
     3121 tcgcgcctgc atccgatgaa ggattgctgt ccaagatatt cggcaagcaa ggtgcactcc
     3181 gcatcggcgc ggccatggtg tatgaccgct tcggcagcga cctgatcacg caatacgatc
     3241 agtacggatc gatcggcatg gccacgtcgg gcaacttccc aacgtcttac agcttcagca
     3301 cgagcccgcg ctacaccggc gcagcgcccg ttctgccgac cccggccgct gagacctttc
     3361 ctgtgacgcc tccaaacatc gctgccatcg cgggagagtt catgggcatc gcgcctaacc
     3421 tgaagccgcc ttactcgtat gtactgaatg ccagcttcgc ccgcgaactg cccggcaagt
     3481 tgaccttcga agtcggctat gccggacgcc tctcgcgccg acttctactc gaaggcgatg
     3541 tctatacgcc gctcgagtac ttcaaagatc ccaagtccgg catcacctgg gagcagaatg
     3601 cgatggcact gcggaagctc cacgactccg gagtgacggt tgcgcaggtc ctggcgaacc
     3661 ccggcattgt tccgaccatg cccttcgtcg aaaacatgtg gccgggactt gccaacctcg
     3721 acgtcaacgg cagcgcttcc gccaactact tcacctgcgt ctacaatgat tacggcggca
     3781 gctatctcga ctgcctgcac gcggtcgatc gcaatccgag cacggcctgg ggcggaacat
     3841 cgaacctggt tggatgcgcc cagatctcca cttacggctg ctacacgttc tttccgctgc
     3901 agggcagctc gatgccgacc tggatgaatg ccggcaccgc cgcttttcac gcattgacgt
     3961 tttctctgcg tcggggactg accagcggcc tcactttcga cttcaattac acctggtcgc
     4021 attcgatcga cgacgggtcg gcggccgaat ccggcgccgg ccagcaaggc gccgccatcc
     4081 agaacatctt caacacgcgc gaattccgcg gctcgtcaga cttcgacatg cgccacaaca
     4141 tcaccgcaaa cgttctttat ccgctgccga tcggacgcgg acgcatgctg ctagtgaatg
     4201 cgcacggctg gctcaacgaa atcgttggcg gatgggctgt cagcagcctg gttcgctatc
     4261 actcgggcgt gccgacgacc gtgggtggcg agctcgccta caacgccaat tactggctga
     4321 gttcgctggc cattgcgacc gcaccggtgt cggcgggcat tcataccgac gagaatggat
     4381 atcccagcat gttctcgagc acatcgatct cgaataactt cgccgacgag ctgccgggac
     4441 acaccggaca gcgcgctgtt ctgcggctgc ctgggatgtt tgtcagcgat atggccgtca
     4501 cgaagaattt cccgctgccg tgggaaggcc agcatcttca attccgcgcc gaagctttca
     4561 acgtgtttaa caatgtgaac tattcgaacc cgagcctcag cctgcaggct ccgggaactt
     4621 tcggagagtt ccagggcgtt ggcgatcccc gcgtcatgca gttcgccctg agattcgagt
     4681 tctaaaagat tcagtccggt tgccccaccc ttgtctcgca tcttttgcgg gacagggtag
     4741 cgcttttgat tctcaaagat gagcgggaag ggcacgagtt aactcgtgcc gattcgatgc
     4801 ccaagaaatt gcggcttcag ccgctgaggg catctgcgtg ggacgtgatt cggggacaca
     4861 cctcagccgc taaagccggg tctttttgac gccgtgatcg gcacgactga agtcgtgccc
     4921 ttcccgcttg cggatgcagc ccccgttcac acacgtctta cgcggtggcc agcacgggtt
     4981 ctggccggaa gctgccggag gcgtgctcaa gatgatgcac cagcatcggc atctggtgtt
     5041 catggca
//
LOCUS       DASPUL010000657         3674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1353107, whole genome shotgun sequence.
ACCESSION   DASPUL010000657 DASPUL010000000
VERSION     DASPUL010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353107"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1085)
                     /locus_tag="VEJ00_15130"
     CDS             complement(114..1085)
                     /locus_tag="VEJ00_15130"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXX02547.1"
                     /translation="MHRITLGDFELTVVSDGAYPLDGGAFFGVVPKIMWSKKVAADER
                     NYVTAGLNSLLIRTGKRTVLVETGMGNKLSERMIKFYGQPAELLDNLAAAGVAPEDID
                     IVINSHLHFDHCGWNTVRDKRGKIIPTFPRAKYYAPEGEWQYARRPSERDAISYISDN
                     YDPLVASGQMTLLKGGEGIVPGISVKTFPGHTAHMQAVIIKGTPGNSTTGKGTASALQ
                     EDAGKSFERARLSVAPSGATKEGEALAHEGTTACYISDLIPTTAHIDLTWGMGFDLYP
                     LDTIESKKQYYAQSIPEKWLTVFTHDPTKPWAYVDKDETGKIVARGA"
     gene            1193..1891
                     /locus_tag="VEJ00_15135"
     CDS             1193..1891
                     /locus_tag="VEJ00_15135"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXX02548.1"
                     /translation="MIQIRPAGERGGGDHGWLKTRHTFSFNDYWDPKWMGFRSLRVIN
                     EDWVAPNTGFPTHPHRDMEIITYVLQGKLEHKDSLGTGSVIMPGDGQRMTAGSGIRHS
                     EFNPSTKEPVHLLQIWIHPERAGLTPSYEQKSFPEKDKRGRLRLIASRDGGDGSVKIN
                     QDAQLYVSLLKPGEEVTHELAAGRHGWLQVARGAVELNGKQLTQGDGAAISDEKKLDI
                     QATRDAEVLLFDLA"
     gene            complement(1963..>3674)
                     /locus_tag="VEJ00_15140"
     CDS             complement(1963..>3674)
                     /locus_tag="VEJ00_15140"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXX02549.1"
                     /translation="RAIGATTLNEYRKYIEKDAALERRFQIVFVGEPNVEDTIAILRG
                     LKEKYEVHHGVRIKDSAIVSAATLSHRYISDRFLPDKAIDLIDEAAASLRIQIDSLPT
                     DIDQLERRATQLEIEKQALKKEDDANSRERLAVIEKDLAEIREKSNALKANWKKEKDL
                     IARTRALKEKIEQLKTEEQAEERKGNLQRVAEIRYSLIRQAEDELAKLTKQMESKGQA
                     RMLKEEVDEEDVARIVSKWTGIPVSKMLEGEVKKLVTMEERLRQRVVGQDAALERVAN
                     AIRRSRAGLSDPKRPIGSFIFLGPTGVGKTELARALAEFLFDDEHAMLRIDMSEYMEK
                     HSVARLIGAPPGYVGYEEGGQLTEQVRRRPYAVVLFDEIEKAHPDVFNVLLQMMDDGR
                     LTDGKGRVVDFKNTIIIMTSNIGSSFLQAEGLRSEAEFEKASEQVMNQLHAHFRPEFL
                     NRVDDIIVFRPLGKDQLVKIIELRLEDVRRLLADRKISLELTDAAKDLLFTQGYDPNY
                     GARPLKRAIQKLVQDPLALKILDGEVLHGDHVVVDADRKAGKMKFEVSQRVGEKATTK
                     TKR"
BASE COUNT          642 a         1066 c         1154 g          812 t
ORIGIN      
        1 caaggaaatt tacgagtcga acggcgcgcc ggaagttctg cggcgtgtgg aggaattctt
       61 cgaagcaagg ttgtgcggcg cgagcgccct cgcccgcgaa gggtgattga agctcatgct
      121 cctctcgcca cgatcttccc ggtctcatcc ttgtctacat acgcccatgg cttggtcgga
      181 tcgtgcgtga acacggtcag ccacttctcc gggatcgact gcgcgtagta ctgcttcttg
      241 ctctcgatgg tgtcgagggg atacagatcg aatcccatgc cccaggtgag gtcgatgtgg
      301 gcggtggtgg ggatgagatc ggagatgtag caggccgttg tgccctcatg agctaaagcc
      361 tcaccttctt ttgtggcgcc tgatggcgcg actgaaagcc gcgccctttc aaagctcttc
      421 cccgcgtctt cctgcaaggc cgaagccgtg cccttcccgg tcgtgctatt gccgggggtc
      481 cctttgatga tcacggcctg catgtgcgcc gtgtgacccg gaaaggtttt caccgagatg
      541 ccgggaacaa ttccttcgcc gcctttgagc agcgtcatct ggccgctggc gaccagcgga
      601 tcgtagttgt cggagatgta gctgatcgcg tcgcgctcac tgggccggcg agcatattgc
      661 cattcgcctt cgggggcgta gtacttggcg cgggggaagg tcggtatgat ctttccgcgt
      721 ttatcgcgga cggtgttcca tccgcaatga tcgaagtgca ggtgcgagtt gatgacgata
      781 tcgatgtctt cgggagcgac gccggcggca gccagattgt cgagcagctc cgcaggctgg
      841 ccgtagaact tgatcatgcg ctcggagagt ttgttgccca tgcccgtctc gaccagcacg
      901 gtccgtttgc cggtgcgaat cagcagtgag ttcagcccgg ccgtgacgta gttgcgctcg
      961 tcggccgcca ccttcttcga ccacatgatt ttcgggacga ctccgaagaa ggcgccgcca
     1021 tcgagggggt aggcgccgtc agagacgacc gtgagttcga aatcgccaag ggtgatccgg
     1081 tgcatgcttt cagtgtgcca cacgcgttcg cccgctttga acccttcgtg gtcgctgcgg
     1141 catccaatgt ttatcgatcc gttaatacct gcatttcaat cggagaaaag ccatgattca
     1201 gatacgtcct gcgggcgagc gcggtggcgg tgaccacggc tggctcaaaa cccgccacac
     1261 cttcagcttc aacgactact gggacccgaa gtggatggga ttccgctcgc tgcgcgtgat
     1321 caacgaggac tgggtcgcgc ctaacacggg ttttcccacg catcctcacc gcgacatgga
     1381 gatcatcact tacgtactcc aaggcaagct cgaacacaaa gacagcctgg gcacgggctc
     1441 ggtgattatg cccggcgatg gccagcgcat gacggccggc agcggcattc ggcacagcga
     1501 gttcaatccg tcgacgaaag aaccagtgca tttgctgcag atctggattc atccggagcg
     1561 cgccggcctg acgccgagtt atgagcagaa gtcctttccg gaaaaggata aacgcggccg
     1621 tctgcgcctg atcgcctcgc gcgatggcgg cgacggctcg gtgaagatta atcaggacgc
     1681 gcagctctac gtctcactgc tgaagccggg cgaagaagtg acgcacgagc tcgccgcagg
     1741 gcgtcacggc tggctgcagg ttgcgcgggg cgcggtggaa ctgaacggca agcagttgac
     1801 gcagggcgac ggcgcggcca ttagcgacga gaagaaactg gacatccaag cgactcgtga
     1861 cgcggaggtg ctgctgttcg acctggcgta agggcgattc tcgccacgga gacacggagc
     1921 taaccagcgc aattttccgt gtctctgtgc ctccgtggtt ggttaacgct tggtttttgt
     1981 cgtagctttc tctcccacgc gctggctcac ctcgaacttc atcttccccg ccttcctgtc
     2041 ggcgtcgacc acgacgtggt cgccgtgcag cacttcgccg tcgagaatct tcagcgccag
     2101 cgggtcctgc accagcttct ggatggcacg cttcagcgga cgggcgccgt agttcgggtc
     2161 gtaaccctgg gtgaagagca gatctttcgc cgcgtcggtg agctcgagcg aaatcttgcg
     2221 atcggcgagc aggcggcgca cgtcttcgag ccgcagctcg atgatcttga ccagctgatc
     2281 tttgcccagc gggcggaaga cgatgatgtc gtcgacacgg ttcaggaact cgggccggaa
     2341 gtgggcgtgc agctggttca tcacctgctc agacgccttt tcgaactcag cttcggaacg
     2401 aaggccctca gcctgcaaaa aagaagaccc gatgttcgac gtcatgatga tgatcgtatt
     2461 cttgaagtcg accacgcggc ctttgccgtc ggtgaggcgg ccgtcgtcca tcatctggag
     2521 gaggacatta aacacgtcgg ggtgcgcctt ctcaatttcg tcgaaaagca cgaccgcgta
     2581 cgggcggcgg cggacctgct cggtgagctg cccgccctct tcgtagccga catagcccgg
     2641 aggcgcaccg atcagccggg cgaccgagtg cttctccatg tattcggaca tgtcgatgcg
     2701 tagcatggcg tgctcgtcgt cgaagaggaa ttccgcgagc gcgcgggcga gttcggtttt
     2761 gccgacgccg gttgggccca agaagatgaa agatccgatc gggcgcttgg gatcgctcag
     2821 gccggcgcgc gagcggcgaa tcgcgttggc gacgcgctcg agtgcggcgt cctggccgac
     2881 aacgcgctgc cgcaggcgct cttccatcgt gaccagcttt ttgacttcgc cttcgagcat
     2941 ctttgagacc ggaatgccgg tccacttgga gacgatgcgg gcgacgtctt cttcgtcaac
     3001 ttcttctttc aacatgcggg cctggccctt gctctccatt tgcttggtga gcttggccag
     3061 ctcgtcttcg gcctggcgaa tcaggctgta gcggatctcg gcgacacgct gcaggttccc
     3121 tttgcgctct tccgcttgct cttcggtctt gagctgctcg atcttctctt tgagggcgcg
     3181 ggttcgggcg atgagatctt tttccttttt ccagttggct ttcagcgcgt tcgatttctc
     3241 gcgaatctcg gccaggtctt tttcgatgac cgcgagccgc tcgcgcgagt tcgcgtcgtc
     3301 ctctttcttc agcgcctgct tctcgatttc gagctgcgtc gcacggcgtt cgagctggtc
     3361 gatgtcggtt ggcaaagagt cgatctggat gcgcagcgac gcggccgctt cgtcgatcag
     3421 atcgatggct ttgtcgggaa ggaaacggtc ggaaatatag cgatgcgaga gcgtcgcggc
     3481 cgagacgata gctgaatctt tgatgcggac accgtgatgg acttcgtact tttccttcag
     3541 gccgcgcaga atcgcgattg tgtcttcgac attcggttcg ccgacgaaga ctatctggaa
     3601 gcggcgttcg agcgcggcgt ctttctcgat gtacttgcgg tattcgttga gcgtggtggc
     3661 gccgatagcg cgca
//
LOCUS       DASPUL010000658         4504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1353601, whole genome shotgun sequence.
ACCESSION   DASPUL010000658 DASPUL010000000
VERSION     DASPUL010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353601"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..462
                     /locus_tag="VEJ00_15145"
     CDS             229..462
                     /locus_tag="VEJ00_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXX02550.1"
                     /translation="MTTLAWKRVLSWEVSEAMRKGRITKSEMAKRMGTSRSQLERLLD
                     PQNPNVLLETVQKAATAVGKRLTIGLTDAPRAP"
     gene            complement(688..840)
                     /locus_tag="VEJ00_15150"
     CDS             complement(688..840)
                     /locus_tag="VEJ00_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02551.1"
                     /translation="MAISKIHAVTPAGMLPGLSLFSKQSEIGSTLQAYSVKGTRSDRL
                     TFEEMV"
     gene            complement(845..1081)
                     /locus_tag="VEJ00_15155"
     CDS             complement(845..1081)
                     /locus_tag="VEJ00_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02552.1"
                     /translation="MFPLVQSGRPASEPTFHAETISHVETETGDVCGTRSQLQGLHAM
                     EDTVKLGCSRFRGKKECGWDQNQKSIRRYQRHAP"
     gene            1364..1744
                     /locus_tag="VEJ00_15160"
     CDS             1364..1744
                     /locus_tag="VEJ00_15160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HXX02553.1"
                     /translation="MTKTAVTSRELAPPVGPFSQAIEVGGFLYVSGQVGQDPATGKVV
                     EGGIEAETERVLRNLAAVLKAAGKSFDHVARAGVYLTRMSDFAAMNGIYAKYFSQPFP
                     ARTTIAVAALPLGACVEIDLVVKA"
     gene            1768..2676
                     /locus_tag="VEJ00_15165"
     CDS             1768..2676
                     /locus_tag="VEJ00_15165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HXX02554.1"
                     /translation="MKYPFHIVDVFSSTPFGGNQLAVLPKAAGISTEGMQKIAREFNF
                     GETTFVLPKNDPANTCRVRIFSPRAELDFAGHPTVGTACALVTKERERFADPIRLTLE
                     ENIGPVTVDVAQRDGGLYGTLTLSAKVETSTDAPSPTDLAAVLSIEPAEVRQVFFAGV
                     GLRFCFAQLSSNEVVDRAVINRAAWGATLVRAWSPQVFLFSGNLRDGGKLYARMFAPA
                     LGVEEDPATGSACAGLVGAMASQPDFGGSTYRLSILQGVSMGRRSEIEAEARKSGSVV
                     TSVSVGGATSYIASGEIEVPPSALVS"
     gene            2798..3562
                     /locus_tag="VEJ00_15170"
     CDS             2798..3562
                     /locus_tag="VEJ00_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02555.1"
                     /translation="MRTMAMVIAVVVTLGSAAIAMDNAGNSNAATAAVADPSRPDSDT
                     SRDVDRKPVQSLEFAGVKSGDTVADYVADTGYFTRLFASVVGSKGHVYAVQPTGFFKY
                     PNFVKAVGDLQSYPVTHPNVTVTIAAALEGLKFPEKLDLFWISQNYHDLHDEFMGPVD
                     IAAFNKAVYSALKPGGLYVILDHSAAQGAPANVTETLHRIEASTVRREVEAAGFKFDS
                     ESSILANPADPRTAKVFDPSIRGHTDQFLLKFRKPK"
     gene            complement(3764..>4504)
                     /locus_tag="VEJ00_15175"
     CDS             complement(3764..>4504)
                     /locus_tag="VEJ00_15175"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HXX02556.1"
                     /translation="RTETLKSLTASDAEPHGLLRIGLSHPLAERTLIDPILALTEKYP
                     KVRLRLLSELTVELYRRLLAGELDVAVVLLPEGKAAPPPLLTNIIATDRMEIVQGAAG
                     NVDSDWKSLGKAPWVLNPVGCPLRAALLDRMERDGCTPMIAAEVHNMHLQLAFVQAGY
                     GVGLLPARFIARNRSIDTVRVLRPAAFDLRPSVAIVRAGQLGALGRAVELLEQGFRRL
                     LEAADTTKPMTRHRRGVPATRRAARRPR"
BASE COUNT          903 a         1325 c         1387 g          889 t
ORIGIN      
        1 tgaagagctc tctgacccaa aaccggatcg cccgcgtcct gttctgtata catcagggac
       61 agatggtgct cctgcatggg ttcatcaaga agacacagca gacgcccgag gacgctctgg
      121 acctcgctgt gaggcggcag aaggaggtta gaggtggcaa agcggcgaaa taaacatgtg
      181 ggctcttcgt tcgatgagtt tctgcggacg gaggggcttt acgaggaagt gacaactctt
      241 gcttggaagc gtgtgttgtc ctgggaagtc agtgaagcga tgcggaaggg gcgtattacc
      301 aagagcgaga tggcgaaacg gatggggacc agtcgcagcc agcttgagcg actgctcgat
      361 ccacagaacc ccaacgtcct gctcgagacc gtacagaagg ctgcgacagc cgtcggaaaa
      421 cggctcacga ttggactgac agacgcgcca cgcgcgccct aattagaaat ggtgggcggt
      481 actgggatcg aaccagtggc ccctgccgtg tgaaacgggg gtagggtgcc tacgaatcaa
      541 cgatatgcga ggcgggtacc cagttgcaac aggcacttag tatcacgtga tgtcatttga
      601 tatcacggaa tgccacaacc gcactgtccc aaaattgttc cagcgaccgg cgtccaccat
      661 ttgcggcggg cggaggctga cagaccctca gaccatctcc tcaaaagtaa gacggtcaga
      721 acgggtgccc ttcaccgagt aggcctgcag cgtgctccca atctcggatt gcttcgagaa
      781 caggctcagt ccgggcaaca taccggccgg cgtaaccgca tggattttgc tgattgccac
      841 gaagctaagg ggcatgccgc tgatagcgac gaatggactt ctggttctga tcccatccgc
      901 actctttctt gcctcgaaag cgcgagcagc cgagtttgac agtgtcttcc atcgcatgca
      961 ggccctggag ctggctgcgg gtgccgcaaa catctcccgt ctcggtctca acatgcgaga
     1021 tggtctccgc atgaaaggtt ggctcagacg ccggccggcc tgactgcaca agagggaaca
     1081 tgggattacg tttctgccgt gcaccggcgt taaccgacct acccagcaga atcgtgcgtg
     1141 ggaagcacga ttctcatgag aataatgaag tagtggaatg ctctgccctg catcaaaatc
     1201 agtacatgcc ttgcgacgcc agttcggcag aggggcgttc cccacgttct gaacggggta
     1261 gcggtcgatg tcggtaagtg cgggtcgggc gaggtgcgcc tgccgggcgg cggcaggcct
     1321 tagttacctt accgtctacc accgcagtcc acaggagttc gctatgacta agaccgcagt
     1381 gacctcgcgc gagctcgcgc caccggtcgg tccgttttcc caggcgatcg aggtcggtgg
     1441 tttcctctac gtcagcggtc aggtcggcca agacccggcg actggcaagg tggtggaggg
     1501 aggtattgag gccgagacgg agcgagtctt gcgcaatctc gcggcggttc tcaaggcagc
     1561 cggtaagagt ttcgatcacg ttgcgcgcgc cggcgtgtat ctcacgcgca tgagcgactt
     1621 cgccgcgatg aatggcatct acgcaaagta cttcagtcag ccctttcctg cccgcacgac
     1681 gatcgcggtg gccgcgctgc cgcttggggc ctgtgtcgag atcgacctcg tcgtcaaggc
     1741 ttgaggcgat gcacgcaggt tgatcttgtg aagtacccgt ttcacatcgt cgatgtgttc
     1801 agttcgacac cattcggcgg taatcagctc gcggtgctcc ctaaggcagc cggcatttct
     1861 accgagggca tgcagaagat tgctcgcgag ttcaatttcg gcgaaacgac cttcgtcctg
     1921 ccgaagaacg atccggcgaa cacctgtcgg gtgcgcatct tctcacctcg ggcggagctc
     1981 gacttcgcgg ggcatcccac ggtcggcaca gcctgtgcgc ttgtaacgaa agagagggag
     2041 cggtttgctg atccaatccg cctcacccta gaggagaata tcggtcccgt aacggtcgac
     2101 gttgctcagc gagacggggg cctctatggc acgctgactc tatcggccaa ggtcgaaaca
     2161 tcgaccgacg caccttctcc taccgatctt gcggctgtct tatccatcga gcccgccgag
     2221 gtgcgccaag tcttcttcgc gggcgtcggt cttcgattct gttttgcgca actgagctcg
     2281 aacgaggtcg ttgatcgggc ggtgatcaat cgggctgcct ggggggccac gctcgtgcgg
     2341 gcgtggtctc cccaggtatt ccttttctca gggaatctac gggatggcgg caagctctat
     2401 gctcgcatgt tcgcgcccgc gctcggcgtc gaagaagatc ctgcgacagg cagcgcctgt
     2461 gccggcctcg tcggggcaat ggcgtcgcag cccgactttg gcgggagcac ctatcgcctt
     2521 tcgatcctgc agggcgtatc gatggggcgc cgaagcgaaa tcgaagcaga ggcgcgtaaa
     2581 agcggaagcg tcgtgacctc ggtcagtgtg ggaggagcca cgtcctacat cgcaagcggc
     2641 gaaatcgaag tgccgccctc cgcgcttgtg tcttaggccg tcgagggcac cgccaattcg
     2701 cgtaaaccgt atcgccgcag cgctcatggc gctcccgcca gccagtgact cagcattggc
     2761 gaataaccaa accagaagat tcacgatagg agtcgacatg agaacgatgg ctatggtgat
     2821 cgcagtcgtc gtcacgcttg gctccgcggc gattgccatg gacaacgcag gtaattcaaa
     2881 cgccgccacc gcggcggtcg ccgatccatc gcgcccggac tcggacacct cacgggatgt
     2941 tgatcggaag ccggtgcagt ccttggagtt cgcgggcgtt aaatcggggg acacggtggc
     3001 cgactatgtg gccgacacag gctatttcac gcgccttttc gctagcgtcg tcggctccaa
     3061 aggtcacgta tatgcggtgc agcccaccgg cttcttcaag tacccgaatt tcgtcaaggc
     3121 agtcggggat ctgcagagct atcccgtcac ccatcccaac gtaacggtga cgattgccgc
     3181 ggcgctcgag ggacttaagt tccccgagaa gctcgacctg ttctggattt cccaaaatta
     3241 ccacgacctc cacgatgagt tcatgggccc ggtcgacatc gccgcgttca acaaggcagt
     3301 ctacagcgcg ctgaagcccg gtgggttata tgtaattttg gatcattccg ctgcgcaggg
     3361 tgctccggca aacgtaaccg agaccttgca tcgcatcgag gcctcgaccg tgcgccgaga
     3421 ggtcgaagcc gcaggattca agttcgactc cgagagttcg attctcgcca atccggcgga
     3481 cccccgcacg gccaaggtgt tcgacccgtc gattcgcgga cacacggatc agttcctcct
     3541 gaagttccgc aaaccgaagt aatgcgcagg gcggcctccc ggggactttc ctaaaggcgg
     3601 ccgcctactg ttcgaaatca aaaagtaggg aagagacagt atgacgccgc attgaggctg
     3661 gccgcgagac cggagtgcgt tggggtgctg atcgtgttca tgatccgtga taccggagag
     3721 cagtatctct cttcggcttt gactgtaggg gccgtgaccg tgactaccgg ggccggcgcg
     3781 ccgcccgccg agtcgcgggg acacctctgc ggtgccgcgt catgggcttc gtggtgtcgg
     3841 ctgcctccag caatcgccga aacccctgtt ccaacagttc gacggctctc cccaaggcgc
     3901 ccagttgccc cgcccgcact attgcgacgc tcgggcgcag gtcgaacgct gcgggacgca
     3961 atactctcac ggtgtcgata gaacgattcc tggcgataaa gcgcgcaggc agcagtccga
     4021 caccataacc ggcctgaacg aacgccagct gcaaatgcat gttgtggact tctgccgcga
     4081 tcattggggt gcacccgtca cgctccatcc gatcgaggag tgcggcccga agcgggcatc
     4141 cgaccggatt gagcacccag ggtgctttgc ctagactttt ccagtcactg tccacgttgc
     4201 ctgcggcgcc ttgaacgatc tccatacggt cagtcgcgat gatgttcgtg agaaggggcg
     4261 gaggcgcggc tttaccttca ggtaaaagta cgactgccac gtcgagctca cccgcgagca
     4321 gccggcgata gagttcaacg gtcagttcgc tcaacagccg cagtcggacc ttggggtatt
     4381 tctcagtcag cgccagaatg gggtcgataa gcgtcctctc ggccaaggga tgcgagagcc
     4441 cgatgcgcaa gagtccgtga ggttcggcgt cgctcgccgt aagagatttc agcgtctccg
     4501 tccg
//
LOCUS       DASPUL010000659         6989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1082696, whole genome shotgun sequence.
ACCESSION   DASPUL010000659 DASPUL010000000
VERSION     DASPUL010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082696"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VEJ00_15180"
     CDS             complement(<1..708)
                     /locus_tag="VEJ00_15180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02557.1"
                     /translation="MITIFTPSFADEADTNAQNLSAKEIVTRLDPEKAAVTMLYEGAV
                     DPRIAARPNTKLLPWSKHGNTLRILWRILRDVPDVYFHPREGPLDAAFLALRRHLRLK
                     TALVARVVSGGLYSQPYSPARVRNISEADAVFANNTYLAQLVKEKLGVDAATIHNGID
                     RRYFFPPESGRVQRDGASVLYAGSLRPYKRVPFVVEQAARWPHVQFRIAGIGEEEQLC
                     KSLAAERKCGNVEFLGHL"
     gene            complement(739..2253)
                     /locus_tag="VEJ00_15185"
     CDS             complement(739..2253)
                     /locus_tag="VEJ00_15185"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HXX02558.1"
                     /translation="MGKHKSIVLVTVDCLRADHVGFMGYACPTTPFIDTLAAESFVVP
                     NAIVAGAPTYYSFPAILASRYPLIMGRDVLGPAPGEPTLASTLKKAGYATAAFGAANP
                     YVSSRFGYEDGFDEFRDFLSDAPAPLTDSPTNDVPGSGWKSRLNRRLERARPAMGPLR
                     LVYDELYFRYCQRVTPVADSLDALRRFPAADLIVDEACNWLSSVGEAPFFLWLHLMDP
                     HSPYYPKDAALRLMGHGPVEPYRARYLNSYWNRSDLGPRRLARHRDEIIELYDAGIRW
                     VDTQMARLVEMLRNAKRWENCILAFTADHGEEFLDHGGRYHPPSRLMEELIHVPLLLR
                     VPGYTKTEVTKAPFSLLHIAPTLLDAAELAIPRAFQGCSYWPQVWQGTGFDSVAISES
                     VASCTNPFRRENRLGPRVLSVREERYKLVLHFEPPAEDLYDLEADPGEQAPLAPSAQH
                     PARRRLLEVAHAHLQRSIGRRDERARLRARLREVQLEWRKPADKASSTVSQPAH"
     gene            complement(2254..3357)
                     /locus_tag="VEJ00_15190"
     CDS             complement(2254..3357)
                     /locus_tag="VEJ00_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02559.1"
                     /translation="MVLLQSDDWGRVGVRDREGMEQLRSAGLTLGERPYDFYSLETAE
                     DVGALAEVLRRHRDSTGSSPCLQMNLILANLDFARMRVDDFRQIHLLPLAEGLPQGWN
                     RPGLTASYRAGIAEGLFRPALHGSTHFCRSAVERALKGDKDRTRFLSTLWQAGTPYIH
                     WRMPWIGYEYWDPEKSEDERFLSAEMQGQSIGQSVGAFAKLFSTLPHSACAPGYRADD
                     NTHRAWALHGIRVAQHGSGVLFPPHFDRHGLLHLCRTVEFEPAVDPAFSLEHCIRCAE
                     TCFEQGLPAIVSVHSINFHSTLKDFRGSTLPVLDEFFSVLESKHPDLLYLHDDDVREL
                     VEKGACTRRHGVVEMNVVRRNFTKARLARQGGA"
     gene            complement(3452..4147)
                     /locus_tag="VEJ00_15195"
     CDS             complement(3452..4147)
                     /locus_tag="VEJ00_15195"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXX02560.1"
                     /translation="METLDRVNREGRERLYPSLTNPSWLVLRRRRGIFEKWLSRIEGN
                     ALLVLDVGGRIQPYRALLENRLHSYVALDLRQTPLVNVVARGEQIPLPADQFDLVLCT
                     QVLEYVPEPAAVIAEIHRVLKPGGYLLLSVPSASPGDADGDSWRFLPAGLRHLLAGFR
                     QFEIVPEGSSVAGLFRNLNVCLSIFVRYPALRSVLHWTVCPVVNLCGEMLERVAHSQN
                     SQFAANYSACARK"
     gene            complement(4199..4396)
                     /locus_tag="VEJ00_15200"
     CDS             complement(4199..4396)
                     /locus_tag="VEJ00_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02561.1"
                     /translation="MTEMREIRLPADLCAAAEKKFNHTFGSLEELLTFILRDLSSDEA
                     SQADQAEQRIIEQRLRELGYL"
     gene            complement(4393..5973)
                     /locus_tag="VEJ00_15205"
     CDS             complement(4393..5973)
                     /locus_tag="VEJ00_15205"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HXX02562.1"
                     /translation="MNDQELKAPPDSAAANQQFRSQVGHISRQSGVFFAGTIFTAGLA
                     YLFKVYLARVLGAEYLGLYALGMTLIGFIGIFNTMGLPQSAVRFVAAYHAAGKFKELH
                     ALVWGGAGMLLAANAVLAVVLLPFGRLVAARVYHSPALVPYLPLFAMILLFGVVNTFY
                     GRVLAGYRDLKLRTLIVNFIGSPLTMLLGVLLISAGMRLRGYLIAQILSAVLVGLLLL
                     LAVRRFTPRAAQLWAQSSLYPRKEVWSFSAAMLGINLLEFIMSQVDKIALGYYRNARE
                     VGIYSVAAALVVYVPLVLSSVNQIFAPTIADLHTRGEYGLLARLFQSLTKWIIGLTLP
                     LALVIIVFARPLMRIFGHDFEGGWPILVIGTFGQLVNCGVGSVGYLLLMSGNQNRLMR
                     VQAAMAVVMVALSAALIPRWGVVGAAVAAAATTVGVNVWNLLEVRAALALSPYNRGYF
                     RLLWPAAVTLALMLGLSRYSHIFHHDWLAVGTALALAYALFAGIVLLTGLDSDDRMIA
                     TAIWSRVRGSFRGRGAEA"
     gene            complement(5970..>6989)
                     /locus_tag="VEJ00_15210"
     CDS             complement(5970..>6989)
                     /locus_tag="VEJ00_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02563.1"
                     /translation="AYAKRAAAVEMVCLTDRWNKVASDLFSIPELRTLFRAGVLDRIP
                     VWLRDGLCARDAWRGVIEGEPVSGVLCGDDSNLYTRLPVVLAARRGIPTADFHHGALD
                     GRYLLKDLPCDVYLAKNEMERDYLLRVCNIPGERVVIGAPPHSQLGLSAEHTEPRRNS
                     AIFFSEPYESAGMRGEEVYREILPALCRLVRQNGRELILKLHPFEIASHRNKILHDVL
                     TPDDRKQITVIAGPLTNDLISQAWFGMTVESSTVLDCVQNGVLCFLCAWMKVSPYEYV
                     EQYDRFGIGQVLANVEQIGEIPARLAEFHSRMLPSPMLSTPADPAMLRAWLTGELRDR
                     ARPAS"
BASE COUNT         1602 a         2130 c         2017 g         1240 t
ORIGIN      
        1 cagatgaccc agaaactcca cattcccgca cttacgttct gcggcgagac tcttgcacag
       61 ctgttcctct tcgccgattc cggcgatgcg aaactgcacg tgcggccagc gcgcggcctg
      121 ctcaactaca aaaggcacgc gtttgtatgg acgcagcgac ccggcataaa gaaccgacgc
      181 gccgtcgcgc tgcacccttc cggattctgg agggaaaaaa tatcgacggt cgatcccatt
      241 gtggatagta gcggcgtcaa cacccagctt ctctttcacg agctgcgcaa ggtaagtgtt
      301 gttcgcgaaa acagcatctg cctcgcttat gttgcgtact cttgccggcg agtatggctg
      361 cgagtacaaa ccaccggaga ccacgcgcgc gacgagcgca gtcttcaacc tcaggtggcg
      421 gcgcagagcc agaaacgccg catcgagcgg gccttcccga ggatggaaat agacatcggg
      481 aacatccctc aaaatccgcc acaggattcg cagggtattt ccatgtttgc tccaggggag
      541 cagcttggta tttggcctcg cggcgattcg cggatccacg gcgccctcgt aaagcatagt
      601 cacagcggcc ttttcgggat cgaggcgcgt cacgatctcc ttggcagaaa ggttctgcgc
      661 attggtatcg gcttcgtccg cgaacgaagg cgtaaaaatc gtaatcattc tagtgccagg
      721 atgccggtgc caagatggct agtgcgcagg ctgggaaact gtcgaagaag ccttgtcggc
      781 cggttttctc cattctagct gaacttctct caagcgggct cgcagcctcg cgcgctcgtc
      841 gcgacggcct atcgaacgtt gcagatgcgc gtgcgccacc tccagcagcc gtctgcgcgc
      901 cggatgctga gcactcggcg caagcggcgc ctgttctccc gggtcagctt caagatcgta
      961 gagatcttct gccggcggct caaaatgcaa aacgagcttg taccgctcct cacgcacaga
     1021 caatacccgc ggtcccagcc ggttctcgcg gcggaacgga ttagtgcaac tcgccacact
     1081 ctccgagatc gcaaccgaat cgaagcctgt cccttgccag acctgcggcc aatagctgca
     1141 gccttgaaag gcacggggaa tcgctaactc agccgcatcc agcaacgtcg gagcaatatg
     1201 cagcaggcta aacggcgcct tggtcacttc cgtctttgtg taacccggaa cgcgcaacaa
     1261 cagcggaacg tgaatcaatt cctccatcag ccgtgatggc ggatgatagc ggccgccgtg
     1321 atccagaaac tcttctccgt ggtcggcggt gaaggcaaga atgcagtttt cccatcgctt
     1381 cgcattccga agcatctcga ccaggcgtgc catctgcgtg tccacccaac ggattccggc
     1441 gtcgtagagc tcgatgatct catccctgtg gcgggccagc cgccgcgggc cgaggtccga
     1501 tcggttccag tacgaattca gatagcgcgc acggtaaggc tcgacggggc cgtgtcccat
     1561 aagccggagc gcagcgtctt tgggatagta aggcgagtga ggatccatca ggtgcagcca
     1621 caggaaaaac ggcgcttcac ccaccgatga caaccagttg caggcttcgt ccacaatgag
     1681 gtcggcagca ggaaaacggc gcaacgcatc gagcgaatcg gcgacggggg tgacccgctg
     1741 acaatatcga aaatagagtt cgtcgtagac caacctcagc gggcccatgg ccggacgagc
     1801 tctctctaac cgccggttga gacggctttt ccaaccgctg ccgggaacgt catttgtcgg
     1861 actatcggtg aggggcgccg gggcgtcgct gagaaagtca cggaactcgt caaagccgtc
     1921 ctcatagccg aagcgcgagg agacgtaggg gttagcggcc ccaaatgctg ccgtggcata
     1981 gcccgctttt ttcaaagtcg acgcgagagt cggttcgccc ggggcgggtc ccagcacgtc
     2041 gcggcccata atcagcggat agcgggaagc cagaatcgcc gggaacgaat agtaggttgg
     2101 cgccccggcc acaatggcgt tgggaacaac aaaactctct gcggccagag tgtcgataaa
     2161 cggagtcgtg gggcatgcgt agcccataaa gccgacatgg tctgcgcgca ggcaatcgac
     2221 ggtcaccaag acaatcgact tgtgctttcc catctacgct ccaccctgcc gcgccagccg
     2281 ggctttagtg aaattgcgtc gaaccacatt catctcaacc acaccgtgcc gacgcgtgca
     2341 agcgcccttc tcaaccaact cccgcacgtc gtcatcgtga agatacagca gatcgggatg
     2401 ctttgactcg agaaccgaaa aaaactcgtc cagaaccggg agcgtgctgc cgcgaaaatc
     2461 cttcagcgtg gaatgaaagt tgatggagtg taccgagacg attgctggaa gaccctgctc
     2521 gaaacaggtt tctgcgcagc ggatgcagtg ttccagcgag aaagcaggat cgaccgccgg
     2581 ttcgaattcc accgtgcgac agagatgaag caagccgtgg cgatcgaaat ggggtgggaa
     2641 gagaacaccc gacccatgtt gcgccacacg aattccgtgc agagcccagg cgcgatgagt
     2701 gttgtcatcg gcgcgataac ccggagcgca agcagaatgc ggaagcgtcg aaaacaattt
     2761 agcaaatgca ccgaccgact ggccgatcga ctgaccctgc atttccgcgg acagaaatct
     2821 ctcgtcctcg gatttctccg gatcccagta ttcgtagccg atccatggca tgcgccagtg
     2881 aatgtatggc gtgcccgctt gccagagagt gctcaaaaag cgagtgcggt ccttgtctcc
     2941 tttcaaagcg cgttcgaccg cggaccggca gaagtgggtc gatccgtgca gagctggacg
     3001 gaatagccct tcggcaattc ccgctctgta ggacgcggtc aggcccggcc gattccaacc
     3061 ctgaggaagg ccctcagcga gcggaagaag atgaatctgc cggaagtcat cgacccgcat
     3121 cctggcgaaa tccagattcg cgaggatgag gttcatctgc aggcagggac tgcttccggt
     3181 cgaatcccgg tgccgtctta acacttctgc caaagcaccc acatcttcgg cggtctcaag
     3241 agaatagaaa tcgtaaggac gctcgccgag cgtgagcccg gcagaccgca attgttccat
     3301 tccttcgcgg tcgcgcaccc cgacgcggcc ccaatcgtcg ctctgcaata aaaccatggg
     3361 acgatcaaag tgaaagcccg cggtaggaag aaaatttcgg caacgccgga ggagttttcg
     3421 agccagattc attttcttga ggtccgggcg ttcatttccg cgcgcaggcg ctgtagttgg
     3481 ctgcgaactg actattctga ctgtgcgcaa cgcgttccag catctcgcca cacaggttta
     3541 cgaccgggca cacggtccag tgaagcactg agcgcagcgc cggatagcga acaaaaatgc
     3601 tcaggcagac gttgaggttg cgaaaaagac cggccacgct gctcccttcc gggacgattt
     3661 cgaactgtct gaagcccgcg aggagatggc gcaagcctgc cgggagaaac cgccaggaat
     3721 ctccgtcggc gtccccggga ctggcagaag gcacgctcag cagaagatat cctccaggct
     3781 tgagcacgcg atggatctcg gcaatcacag cggcaggctc aggaacgtac tccagaacct
     3841 gcgtgcaaag caccagatca aactggtcag ccggcaacgg aatctgctcg ccccgcgcta
     3901 cgacattgac caggggcgtc tgccgcagat cgagcgctac atagctgtgc aggcgatttt
     3961 ccagcagcgc ccgatacggc tgaatgcgcc cgcccacgtc aagcacaagc agcgcattac
     4021 cctcgatgcg ggacagccac ttctcgaata tcccgcggcg gcggcgcagc accagccagc
     4081 tgggattggt caacgaggga tacagccgct ccctgccttc acgattgacc ctatccaatg
     4141 tttccatgcc gtctgactct gcgaggctcg tgaaacgaga gcgaggtcca gcttcggctc
     4201 aaagatagcc aagctcccgc agccgctgct caatgatgcg ttgctctgcc tggtcggcct
     4261 gggaggcttc atcgctcgac aggtcgcgca ggatgaaagt cagcagttct tccaggctgc
     4321 cgaaggtgtg gttgaacttc ttctcggcgg ctgcacatag gtctgccgga agtcgaatct
     4381 ccctcatctc cgtcatgctt cagccccgcg gccgcgaaac gaaccgcgta cccgcgacca
     4441 gatcgcagta gcaatcatgc ggtcatcgga atccagtcct gtcaggagaa caatgccggc
     4501 aaacagcgca taggccaggg cgagcgccgt gccgaccgcg agccagtcgt gatgaaaaat
     4561 gtgcgaatac cggctcaacc cgagcatcag agcgagcgtc accgccgccg gccaaagaag
     4621 ccggaaatag ccacggttgt aaggactgag agcgagggcc gcgcgaacct ccagcagatt
     4681 ccagacgttg actccgacgg ttgtagcagc ggcggcaacc gcagccccta caactcccca
     4741 gcgcggaatc aatgcggcgc tcagggcgac catcacgact gccatagctg cctgcaccct
     4801 cataagccgg ttctgattgc ccgacatgag cagcaagtat cccaccgaac cgactccgca
     4861 gtttaccagt tggccgaagg taccgatgac cagaatgggc cagcctccct cgaagtcatg
     4921 gccaaaaatg cgcatgaggg gacgggcaaa aacgatgatc accagcgcga gcggcaaggt
     4981 aagcccaatg atccacttgg tgagcgactg aaacaaacgc gccagcaagc catattcgcc
     5041 gcgggtgtgc aggtcggcga tggtcggcgc aaagatctga ttcaccgagc tcaagaccag
     5101 cggaacatac accaccagcg ccgccgccac cgaatagatg cccacctctc gcgcattccg
     5161 gtagtagccc aaagcgatct tgtccacctg actcatgatg aattcgagca ggttaattcc
     5221 caacatggct gccgagaacg accagacttc cttccgcgga tacaagctgg actgggccca
     5281 tagctgcgcc gcgcgcggcg tgaaccgccg caccgcgagc agtaacagca ggcccacgag
     5341 gaccgcgctg agaatctgcg ctatgagata gccacgcagg cgcattcccg ccgaaatcag
     5401 taagacgccg agaagcatgg tcaaaggact gccgataaaa tttacaatga gcgttcgaag
     5461 cttcaggtcg cggtagccgg ccagcaccct tccgtagaac gtgtttacaa ccccgaacag
     5521 caggatcatg gcgaacagag gcagatatgg caccagcgca ggggagtgat acacacgcgc
     5581 ggctaccagc cggccgaagg gcaagaggac aactgccagc accgcattcg ctgccagcag
     5641 catgccggcg ccgccccaaa cgagggcgtg caattccttg aactttcccg cggcgtgata
     5701 ggcggccacg aagcgcacgg ctgactgagg caatcccatg gtattgaaga tcccgatgaa
     5761 tccgattaac gtcataccca gggcatacaa accgagatat tccgcaccga ggacacgggc
     5821 gagatacacc ttgaataggt aggcgaggcc cgccgtgaaa atcgtgccgg caaagaacac
     5881 tccggactgg cgcgagatgt gccctacctg cgagcgaaac tgctgattcg cagctgcgct
     5941 atcaggagga gctttcagtt cctgatcgtt catgaggccg gcctcgcgcg gtcccgcagt
     6001 tcaccggtca gccacgcccg taacatggcc gggtcggccg gcgtcgaaag cattggcgag
     6061 ggcagcattc gactatgaaa ttccgctagc cgtgcgggaa tttccccgat ctgctctacg
     6121 ttggcaagga cctgcccgat tccaaaccgg tcatactgct caacgtattc gtaaggcgaa
     6181 accttcatcc atgcacacag gaaacagagg acaccgtttt gcacacagtc gagcactgtc
     6241 gacgactcta ccgtcattcc aaaccaagct tgtgagatca gatcgttggt aagggggcca
     6301 gcgatcactg tgatctgctt ccggtcgtcg ggagtcagga cgtcgtgaag aattttgttg
     6361 cggtgggagg caatctcaaa aggatgcagt ttgaggatca gctcacgccc gttctgccgc
     6421 accagccgac agagagcggg caaaatctcg cgatagacct cctcgccgcg catgcctgcg
     6481 gactcgtagg gctcggagaa gaaaatggct gagttccttc gcggctcggt gtgctccgcg
     6541 ctcaggccaa gctgcgaatg cgggggcgct ccgatgacca cccgctcccc cggaatattg
     6601 cagacccgca gcaaatagtc ccgttccatt tcgttcttgg caaggtatac gtcacagggt
     6661 aagtctttca gcaagtaacg cccatccagc gcgccatgat gaaaatcggc ggttgggatt
     6721 ccccgccgcg cagccagcac aaccggcaac cttgtgtaaa gattgctgtc gtcgccgcaa
     6781 agaacgccac tcaccggttc gccctcaatc actccgcgcc acgcatcgcg ggcacagagg
     6841 ccgtcgcgca gccacacggg gatccgatcc agtaccccgg cacggaagag ggttcgtagt
     6901 tccgggatcg aaaacaaatc ggaggctacc ttgttccagc ggtcggtcag gcataccatt
     6961 tcgacggccg cggcgcgttt cgcataggc
//
LOCUS       DASPUL010000660         3429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1225323, whole genome shotgun sequence.
ACCESSION   DASPUL010000660 DASPUL010000000
VERSION     DASPUL010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225323"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VEJ00_15215"
     CDS             <1..1063
                     /locus_tag="VEJ00_15215"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXX02564.1"
                     /translation="FAKAGDVAGKVLLVHTTVLKTWADLFAEYSQAPPVIDRAVKAKA
                     KALAFMATREHDILYRHTNAGAGEIDKLPMVIVAREDGERIARLLADGHLVWADLSIP
                     NQVGGPIRAANVVGEIKGSEKPDEFVILGAHLDSWELGTGALDNGCNAALVIDALRAI
                     KASGVKPRRTIRFILFSGEEEGLIGSRAYSVAHRHELDKAAGVVIYDAGTGKTTGFSI
                     GGRKDVLDTARELIIPLAQFDVKDLIPTMEWGTDHFDFMLEGVPTFVAEQQEANYLEN
                     YHAVSDTYDKVDFAQLKKHVAEAAALSVGLANLPEKIGPRLTRDQIEQTMRDSNSVDE
                     LKADGIWDDWVNGKRGRQK"
     gene            1095..2099
                     /locus_tag="VEJ00_15220"
     CDS             1095..2099
                     /locus_tag="VEJ00_15220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HXX02565.1"
                     /translation="MKAAVPVRFFIQQSKSTINNSLTPPGNPLLVYWLLFNLFALGML
                     LLDLRVFHRPGRSAGFRSALGWSALYILVAAAFAVLLHFWQGHRAALEFVTGYVLELS
                     LSVDNLFVFLVIFRFFAVPEEQQHRVLFWGILGAVVMRGIFIIAGVGLISRFHWLFYG
                     FGVLLIYSGIRVCIAGAHQIDPAANPIVKMLRRWVHVTSDYQGGKFFVRKPGEAALYV
                     TPLLVVLLVIETTDVLFAVDSIPAILGITLNAFIVYTSNVLAILGLRSMYFAVAGLMK
                     AFRFLHYGLAVVLILVGLKMLIADYIHIDIADTLGVVAEVLFISIVASVLYPGKAKSS
                     "
     gene            complement(2179..>3429)
                     /locus_tag="VEJ00_15225"
     CDS             complement(2179..>3429)
                     /locus_tag="VEJ00_15225"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXX02566.1"
                     /translation="STKMIFGREGAQLHNRVFHSQGESTGKEILQALAAKTHSLKHIA
                     AAPFAFTTGLLSDGGRIMGVSLMDEKGVPQDIACSAVLLATGGMGQLYRSTTNPEAAT
                     ADGVALAVRAGAEAGDMEFIQFQPTVLYVKKAPRFLLPDTLLAEGACLRNFELDRFMG
                     KYHPLAELAPLDVVSRAIMHEMEVSRAKDPFVYLDLTHLSAAKVQKRFPRIYATLMQR
                     NLDITEDLIPVRPAAHFAVGGVRTDLDGRTNIAGLYAAGEAAASGVHGANRLPSNALL
                     EGLVFGARAGQAMRQELRTSARPATRPKAAYSNGPVDTVAEETIATIQDLMWNEVGIV
                     RIRMGMSKAIRTLEEMAPRLSHPRTRRAHEAANLRLAGLLVARSALAREESRGAHYRI
                     DYPDHDDRRFLKHSVVKGEAVRFG"
BASE COUNT          609 a          987 c         1096 g          737 t
ORIGIN      
        1 tttcgcgaaa gctggcgacg ttgccggcaa ggttttgctt gtgcacacga cggtgctgaa
       61 gacctgggcc gatctattcg ccgagtattc gcaagctccg cccgttatcg accgagccgt
      121 caaagcgaag gcgaaagcac tcgcattcat ggccacgcgg gagcacgaca tcctgtatcg
      181 gcatacgaac gccggtgcgg gggagatcga caagctgccc atggtgattg tggcgcgcga
      241 ggatggcgaa cgcatcgcgc gactgctggc cgatggccat ctggtgtggg ccgatctttc
      301 gataccgaat caggtgggcg ggccgatccg ggcggcgaac gtggtggggg aaatcaaagg
      361 cagcgagaag cctgacgagt tcgtcattct gggggcgcat ctggattcgt gggaactcgg
      421 caccggcgcc ctcgacaacg gctgcaacgc ggcgctggtc atcgacgcac tgcgggcaat
      481 caaggcttcg ggcgtgaagc cgcggcggac gatccgcttc attttgttct ccggcgaaga
      541 agaggggctg atcggctcgc gcgcttactc ggtcgcgcac cggcacgaac tcgacaaggc
      601 ggcgggcgta gtcatttacg atgcgggcac gggcaagacc acgggctttt ccatcggcgg
      661 ccgcaaggat gtgctcgata ccgcccggga attgattatt ccgctggcgc agtttgacgt
      721 caaagatctg atcccgacca tggagtgggg caccgatcac ttcgacttca tgctggaggg
      781 cgttccgacg ttcgttgccg agcagcagga ggccaactac ctggagaact accacgccgt
      841 ctccgacacc tacgacaagg tagatttcgc gcaattgaag aagcacgtgg ccgaagctgc
      901 ggcgctgagc gtgggactcg cgaacctgcc ggaaaagatt ggcccgcgcc tgacccgcga
      961 ccagatcgag cagaccatgc gcgacagcaa cagcgtggac gagctcaagg ccgacggcat
     1021 ctgggacgac tgggtcaacg gcaagcgggg acggcagaag tgagcggtgg ttgaatcttg
     1081 attgttgtcg ttgaatgaaa gccgccgtac ccgtccggtt tttcattcaa caatcaaaat
     1141 caacaatcaa caactctttg acgccgccgg ggaatccgtt gctcgtctac tggctcctgt
     1201 tcaatctgtt cgcgctgggc atgctgctgc tcgacctgcg tgtctttcac cggccggggc
     1261 gcagtgcagg atttcgctcc gccttgggct ggagcgcact atacatcctg gtggcggcgg
     1321 cgtttgccgt tttgttgcat ttctggcaag ggcatcgggc ggctctggag ttcgtaaccg
     1381 gctacgttct tgagctctcg ctcagcgtcg acaatctttt cgtcttcctc gtgatcttcc
     1441 ggttctttgc cgtgccggag gagcagcagc accgcgtgct gttctgggga attctgggcg
     1501 cggtggtcat gcgcggaatc ttcatcatcg cgggtgtcgg actgatttcg cgcttccatt
     1561 ggctgttcta cgggttcggc gtgctgctga tctatagcgg gatccgcgtg tgcatagccg
     1621 gcgcacacca gatcgatccc gccgccaatc cgattgtgaa gatgctgcgg cgctgggtgc
     1681 atgtaacttc cgactatcag ggcgggaagt tcttcgtgcg gaagccgggg gaagctgcac
     1741 tttatgtgac gccgctgctg gtggtgctgc tggtcatcga gaccacggat gtgctgttcg
     1801 cggtcgattc cattcccgcg atcctgggta tcacgctgaa cgccttcatc gtctacacct
     1861 cgaatgtgct ggcgattctg gggctgcgct ccatgtactt cgccgtcgcc ggtctgatga
     1921 aggcgttccg ctttctccac tacgggctgg cggtggtgct gattctggtg ggattgaaaa
     1981 tgctgatcgc cgactacatc cacatcgaca tcgcagacac gctcggcgtg gtggctgagg
     2041 tgctgtttat ttccattgtg gcgtcggtgc tgtatccggg gaaggcgaaa agcagttgat
     2101 gattgttgaa tgaacccccc gagcctcggg gagcccggtt ttcatttaac gatcaacaat
     2161 cgaaaatcga caatcgtgct atccgaagcg cacggcctct cccttcacga ccgagtgctt
     2221 cagaaatctc ctgtcgtcat gatcgggata gtcgatgcgg tagtgcgcgc cgcggctttc
     2281 ttctcgggcc agtgccgagc gggccaccag caatccggcg aggcgcaggt tggcggcttc
     2341 atgcgcgcgg cgcgtccgcg gatgggacag tctcggagcc atctcttcca gagtcctgat
     2401 cgccttgctc attcccatgc gaatgcgcac gatgccgact tcgttccaca tcagatcctg
     2461 gatggttgcg atggtttctt ccgcgactgt gtcgacgggc ccgtttgagt aagctgcctt
     2521 gggacgagtt gctggccgcg ccgaggtcct gagttcctgc cgcatggcct ggcctgcgcg
     2581 ggcgccgaag accaggcctt cgagcagagc attactcggc agacggttgg ccccgtgtac
     2641 tccgctcgcc gcggcctcgc ccgcggcgta gaggccagcg atgttcgttc tgccatccag
     2701 gtcggtgcgt acgcctccca ccgcgaagtg tgccgctggt cgaaccggaa tcagatcttc
     2761 cgtgatgtcg aggttacgtt gcatcagcgt cgcatagatt ctcggaaagc gcttctgtac
     2821 ttttgcagcg ctgagatgcg tgagatcgag gtaaacaaac ggatccttgg cgcggcttac
     2881 ttccatctcg tgcatgatgg cgcgcgaaac cacgtcgagc ggcgccaact cggccaaggg
     2941 gtggtatttc cccatgaagc ggtccagctc gaagttcctg aggcaggctc cctcggcgag
     3001 cagagtatcg gggagcaaaa agcgcggcgc ttttttcaca tacaacaccg tgggttgaaa
     3061 ctggatgaac tccatgtcgc cggcttcggc ccctgcgcga acggccagcg caacgccgtc
     3121 tgcggtcgcc gcttcgggat tggtcgtgct gcgatagagt tggcccatgc ctccggtggc
     3181 gagcaacacg gccgagcaag cgatgtcctg gggcacgccc ttctcgtcca tcagcgagac
     3241 tcccatgatg cgtccgccgt cgctgagcag gccggtagtg aaagcgaaag gcgcggcagc
     3301 gatgtgcttg agcgagtggg tcttcgcggc aagcgcctgc aggatctctt tgccggtgga
     3361 ctctccttgc gagtgaaata cgcgattgtg gagttgcgcg ccttcgcgtc cgaagatcat
     3421 cttggtgct
//
LOCUS       DASPUL010000661         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_272971, whole genome shotgun sequence.
ACCESSION   DASPUL010000661 DASPUL010000000
VERSION     DASPUL010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272971"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(497..1234)
                     /locus_tag="VEJ00_15230"
     CDS             complement(497..1234)
                     /locus_tag="VEJ00_15230"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXX02567.1"
                     /translation="MNRTSLVICLAASMMATVGCSSKNYVKQQTTPLINKTNELDDMT
                     AKNTKDIKDVDARVQAGLAAVNAKTDAVEQKATTAGQNAAQAQQVADAANARVGVLTN
                     TVANLDNYHAVAETSVKFAFNKDNLTSKAKEALDQLAGNIASTKGYIIALEGGCDSVG
                     PADYNYDLSQRRANSVIQYLASKYSVPAHKIYVIGLGKDKPVETNKTAAGRADNRRVD
                     VRLMTNTTGGNAPASTPATTGSNPSSM"
     gene            complement(1558..1968)
                     /locus_tag="VEJ00_15235"
     CDS             complement(1558..1968)
                     /locus_tag="VEJ00_15235"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXX02568.1"
                     /translation="MKGIADTGFIVAAANRDDNHHAWALNIARAVTEPLLTCEAVLAE
                     AAFHLKSSSYVLSLVEEELLRVDFDLSKNLPELRELARRYADRTPDLADLCLIRMSEL
                     HPRYVVITVDENDFRVYRRNKREAIPLLCPPRMQ"
     gene            2249..>2536
                     /locus_tag="VEJ00_15240"
     CDS             2249..>2536
                     /locus_tag="VEJ00_15240"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXX02569.1"
                     /translation="MDLLQKIRTIPTEPGVYLYKNAEGEVIYVGKAKNLRSRVASYFH
                     EGRWEDAKTGTLVREAVDVDYIVVANNKEALALENNLIKQRKPRFNILLRDD"
BASE COUNT          489 a          759 c          746 g          542 t
ORIGIN      
        1 agacgcttcc ggcgactgcc cgctcggaga agacgaaccg tcaggagttt tgccgggagc
       61 agatgaactc tgcgcccacg ccaccccagc actcagcagc aggaccagga ttacgaaccc
      121 gcgacgcata cagctatttt acctccgcca aaagcgtcgt cgccggagct acgtcgcctc
      181 tacgtagctc ccgttgcggt aggccagcct gccgtggccg tcttatatga ccgccgctgc
      241 cgggcccgct gccgtggaag agtggccctt cagggccacg tcgcggcccc ggaacacagg
      301 gctttggccc ccgtggtcgc cctgtagggg tccttccact cagccgattt tgcaaaagaa
      361 aaaaacaggc cgctcagggg gaggtgttga gcggcctggt tggcgaagtc ctccggggag
      421 gagggtaaac ttctggcttc gagccgtgag cttcgagctt tgaaccacca gctatgatgc
      481 ggtcgactcg cggctcttac atcgacgacg gattgctgcc cgtggtcgct ggagttgacg
      541 ccggtgcgtt gccacccgtg gtgttagtca tcagacgcac atccacgcga cggttgtcgg
      601 cgcggccggc tgccgtcttg ttcgtctcga ccggtttgtc cttgcccagg ccaatcacgt
      661 agatcttgtg cgccggcacg ctgtacttcg acgccagata ctgaatcacc gaattggcgc
      721 ggcgctggct gagatcgtag ttgtagtcgg ccggtcccac cgagtcacat ccgccttcga
      781 gcgcgatgat gtagcccttg gtcgaagcga tattgcccgc cagttggtcg agcgcctctt
      841 tggccttcga cgtcaggttg tccttgttga aggcgaactt caccgaggtc tcggccacgg
      901 cgtgatagtt gtcgaggttg gcgaccgtgt tggtcagcac gcccacgcgc gcgttagcgg
      961 catcggctac ctgctgcgcc tgcgcggcat tctggccggc agtcgtcgcc ttctgctcga
     1021 cggcgtccgt cttggcgttc accgccgcca ggccggcctg cacccgcgcg tcaacatcct
     1081 tgatgtcctt ggtgttcttc gcggtcatgt cgtcgagctc attggtcttg ttgatcagcg
     1141 gcgtcgtctg ttgtttcaca taattcttgc tggagcagcc gacggtcgcc atcatgctgg
     1201 ctgccagaca aatcactaac gaagtgcggt tcataaggtg aggctccttc atcaatccgg
     1261 ttctcgtgcg ccctggtttg gcttagcttc gttgccgggc taaggtggcc ctgcgcacga
     1321 ttttgcaatc tttgaggccc tgggaagtgg ctgactgctt acgagtcccg tcctgacttg
     1381 tgcacggcgg atgccagtgt cgggtattaa gaccctgcgc tgtaagtata tcgggtttca
     1441 agggcttgcg gaagtccgcg tgcagggtcc agcggctgca ccgcccacag ggcaacgcaa
     1501 tccacacacg gcagaatgga aattttatac ggcgaggctc agattgccca tccagcatta
     1561 ctgcatgcgc ggtgggcaga gcaacggaat ggcttcccgc ttgttgcgcc gatagacgcg
     1621 gaagtcattt tcatcgacag ttatgacgac ataacgggga tgaagttcgc tcatccgaat
     1681 taggcagaga tcagccaggt ccggggtgcg atctgcgtag cgtcgagcta gttcgcgcag
     1741 ttcgggcagg ttcttcgaga gatcaaagtc tactctcagc aattcctcct ctaccagaga
     1801 cagcacgtat gagctcgact tcaagtgaaa ggccgcctcg gccaacactg cctcgcaagt
     1861 aagcagcggt tcggttaccg cccgggcgat attgagcgcc caggcgtggt gattatcgtc
     1921 ccggttggcg gccgcgacga tgaaaccggt gtcggcaatt cccttcatga tttggagaat
     1981 cccttgcgcg acgaaagatc ggccggaccc tgaatcgctc cgagtcgccg caggaatcgt
     2041 tgttttccgc tgctctcctt ggctgcctcc agctggtcgc ggacaagtcg tcccatcggg
     2101 atgccagtga cgcgtgaggt ttccttaagc caatccagaa gttcatctgt aagacgaatt
     2161 gttaatgtat gacccatatg cacacattgt aacacatctt cggaatcttg agctccggat
     2221 caatcgcata aaataactcc atccccgaat ggacctcctg cagaaaatcc gcaccatccc
     2281 cacagagccc ggcgtgtatc tgtacaaaaa cgccgaaggc gaggtcatct acgtcggcaa
     2341 ggcgaaaaac ctgcgcagcc gcgtggccag ctacttccac gaaggccgat gggaggacgc
     2401 caagaccggc acgctggtgc gcgaggcagt tgatgtcgac tacatcgtcg tggccaacaa
     2461 caaagaggcg ctggcgctcg aaaacaacct gatcaagcag cgcaagccgc gcttcaacat
     2521 cctgctgcgc gacgac
//
LOCUS       DASPUL010000662         3065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1630388, whole genome shotgun sequence.
ACCESSION   DASPUL010000662 DASPUL010000000
VERSION     DASPUL010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630388"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VEJ00_15245"
     CDS             complement(<1..220)
                     /locus_tag="VEJ00_15245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02570.1"
                     /translation="MTTECPPLFGQHVRLEPLEHRHVGGLVVAAAADPSLYRWSPVPQ
                     GKAETAKYVETALAWRDAGTAVPFAIVRA"
     gene            complement(268..1086)
                     /locus_tag="VEJ00_15250"
     CDS             complement(268..1086)
                     /locus_tag="VEJ00_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXX02571.1"
                     /translation="MPFVDKALARRLESCEEIPQVEYARVFRETRPEIGAAEEEICGG
                     HMIFAGLGSPIGRATGVGLDRGLTADDLDRIEQFYREHKAPSQVDLTPIHEPAVFEMF
                     KQRGYAIAELNNVLYKKLDGSETLTPAPAGCEIRRSRLDESEVTGAIVESAFFPDGAP
                     EAFRGLIAPLYRMERALPFVACVDDKPVACGTGLVIPEHKVFALCGAGTLTEYRGRGL
                     QTGLLRTRMSAAAEHGCEFAVVVTQGGTTSQRNAERLGFQVAYSKVTVVKHLDA"
     gene            complement(1444..2592)
                     /locus_tag="VEJ00_15255"
     CDS             complement(1444..2592)
                     /locus_tag="VEJ00_15255"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXX02572.1"
                     /translation="MKLLRPLLLALVIAGAFFYFTTWRSNGRAGVHPSNWLSRPAEVE
                     ITEAAPGEPLDSEEQNNISVYRKNIPSVVNVTSRALTFDFFYGLVPQEGQGSGFVIDR
                     DGHILTNYHVIADARQVKVTLHNRKSYDAKVVGTDPAHDLAVIQIKAPELVPAVLGDS
                     RNLQVGQKVYAIGNPFGLAGTMTRGIVSSIRPVREPNGAMIDEAIQTDAAINPGNSGG
                     PLMNWHGEVIGINTMILSSVGQNAGIGFAIPINTAKAVLNDLVTLGRVRRPALGVTTI
                     PIGPELADSIGLPADYGLLIIQVTPGGSADLAGLRGGTERAYLGSTPIMLGGDLIVAI
                     DDEKIEDQQDLAQVMNNHRAGDTVKVTIYRNKKKMDVNVALGEARQQV"
     gene            complement(2707..>3065)
                     /locus_tag="VEJ00_15260"
     CDS             complement(2707..>3065)
                     /locus_tag="VEJ00_15260"
                     /inference="COORDINATES: protein motif:HMM:NF022643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HXX02573.1"
                     /translation="RTRLESIAMKPKTLYLALCLAGVAVPYWQFVPWVLQHGLNFALF
                     TRELFSNRIGAFFGMDVVVSAVALLAFSRIEGVRMRIRGRWIVLVAVLTVGVSLGLPL
                     LLYLRERELERQSHVM"
BASE COUNT          611 a          993 c          859 g          602 t
ORIGIN      
        1 gcgcacgcac aatcgcaaac ggcacggcag ttcccgcatc gcgccaggct aaagcggtct
       61 ccacgtactt ggcggtttct gccttccctt gcggaaccgg actccaacga tagagcgagg
      121 gatcggcggc agccgcaacc accagcccgc cgacgtggcg atgctcaagc ggctccagcc
      181 gaacgtgttg gccgaaaagc ggcggacact ctgtcgtcat cgatgcaatg tgcgagcgtt
      241 catcgaacca caacgggtgc cccgagctca agcgtccaga tgtttgacta ccgtgacctt
      301 gctgtacgcc acctgaaacc ccagccgctc ggcattccgt tgcgaagtcg tccccccctg
      361 cgtgaccacg accgcaaatt cacagccgtg ctcggcggcc gctgacatgc gggtccgcag
      421 gagccccgtt tgcagcccgc ggccgcgata ctccgtcagc gtccccgccc cgcacagcgc
      481 aaatacctta tgctctggaa tgaccagtcc cgtgccgcat gccactggtt tgtcgtccac
      541 gcaggctacg aaaggcaagg cgcgttccat ccggtagaga ggggcaatca gcccgcgaaa
      601 cgcttccggg gcgccgtcgg ggaagaaggc gctttccaca atcgctcccg taacttcgga
      661 ctcgtccagc cggctgcgcc ggatctcgca gcccgccgga gccggagtaa gcgtctcgct
      721 cccatccagc tttttataga gcacattgtt cagttccgcg atcgcatacc cgcgctgctt
      781 gaacatctca aacacggcag gctcgtggat cggggtcaga tcgacctgcg agggcgcctt
      841 gtgctcccga taaaactgct cgatgcgatc cagatcgtcg gcggtcaacc cacgatccag
      901 cccgaccccg gttgcccgtc cgatgggcga ccccaggccg gcaaaaatca tgtgcccgcc
      961 gcaaatttct tcctcggccg caccgatttc cggccgtgtc tcccgaaaca cccgtgcata
     1021 ctcgacttgc gggatctcct cgcacgactc tagacggcgc gccagcgcct tatcgacaaa
     1081 tggcatgtca gtattccttt ctgtttgcag aaatcgccag ggctggagcg caggcgacgc
     1141 gcggggcgca cctggctcaa acatgaggag tcggatctaa agaacggcga tcataggcgg
     1201 aaaactagag actagcgtac gatcctgatt gcctgcaagc gccgaatttc ggagcatttt
     1261 tctttgcgct ctttgcggcc ttttcttcgc gatctctgcg gtcaaaggct cttgccgcaa
     1321 agtgcgccaa ggaacggccg cagagagcgc aaagagagac ttactgacga atccgagatt
     1381 gccggttcgt tagagtctcc aggtcgctcg gcaatggtca attgcagatc gaaattcagg
     1441 cagctacacc tgctgccgcg cttcccccag cgccacgttg acgtccatct tcttcttgtt
     1501 gcggtagatg gtcaccttca cggtgtcgcc ggcgcggtga ttgttcataa cctgcgccag
     1561 gtcctgctga tcctcgattt tctcgtcgtc aatcgctacg atcaggtctc cgcccagcat
     1621 gatcggagtg cttcccaggt aggcgcgctc ggtgccgccg cgcagcccgg caagatcggc
     1681 cgatcctccc ggagtcacct gtatgatcag caatccgtag tccgccggca ggccgatcga
     1741 atcggccagt tcgggaccga tcggaatcgt cgtcaccccc agcgccggac gccgcacccg
     1801 ccccaatgtc accagatcat tgagcaccgc tttcgcggta ttgatcggaa tggcgaatcc
     1861 gatcccggca ttctgaccca cgctcgaaag aatcatggta ttgatgccga tcacttcgcc
     1921 gtgccagttc atcagcggcc cgcccgagtt gcccggattg atggcggcat cggtttgaat
     1981 agcttcgtcg atcattgccc cgttgggctc gcgcacgggc cgaatcgaac tgacaatgcc
     2041 gcgcgtcatt gtcccggcca agccgaaagg attgccgatg gcatacactt tctggccgac
     2101 ctgcaggttg cgcgaatctc ccaggaccgc cggaaccagc tcaggcgctt tgatctggat
     2161 caccgccaga tcgtgagccg gatctgtgcc cactaccttt gcgtcatagc tcttgcgatt
     2221 gtgcagcgta accttcacct gccgcgcgtc ggcgatgacg tggtagttgg tcagaatatg
     2281 cccgtccctg tcgatgacga accccgagcc ttgcccctcc tgcggcacca gtccgtagaa
     2341 gaaatcgaag gtcaacgccc gcgaagtcac gttcactacc gacggaatat tcttgcggta
     2401 gaccgagata ttgttctgct cttcgctgtc gagcggttcg cccggggcgg cctcggtgat
     2461 ctccacttcc gccggccggc tcagccagtt cgaaggatgc actccggccc gcccgttcga
     2521 gcgccaggtc gtgaagtaga agaatgcgcc ggcgatcacc agcgccagaa gaagagggcg
     2581 taagagtttc atcgttcatc cacctgaatg aagattactt tgtcaggaag cccaaaggtt
     2641 ccactctgga ttttaatcaa tgtgcccgcg cggcgcactt ctcgatgctg tctgcttgga
     2701 gagtgttcac atcacgtgtg attggcgctc cagttcccgt tcccgcaggt agaggagcaa
     2761 cggaaggccc agtgacacac cgaccgtgag caccgcgact agcacaatcc agcggccgcg
     2821 aattcgcatg cgtacaccct cgattcggct gaatgcgagc agcgccacgg ccgaaacaac
     2881 gacatccatc ccgaagaatg cgccaattcg gttggaaaac aactcgcgcg tgaataaggc
     2941 gaagttcaat ccgtgctgga gcacccacgg cacgaactgc cagtaaggga ccgctactcc
     3001 ggcaaggcag agggccagat acagcgtttt cggtttcatc gcgatcgatt ctagccgcgt
     3061 ccggg
//
LOCUS       DASPUL010000663         6079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1776072, whole genome shotgun sequence.
ACCESSION   DASPUL010000663 DASPUL010000000
VERSION     DASPUL010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1776072"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /gene="hutU"
                     /locus_tag="VEJ00_15265"
     CDS             complement(<1..1063)
                     /gene="hutU"
                     /locus_tag="VEJ00_15265"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HXX02574.1"
                     /translation="MPVETEIQAPVSIRAPRGNSITCKGWQQEAAMRMLMNNLDEEVA
                     ERPQDLVVYGGTGRAARSWEAYHAIVATLKNLENDETLLVQSGKPVGVFKTHEHAPRV
                     LIANSNLVGHWSNWEKFNELERAGLMMYGQMTAGSWIYIGSQGIVEGTFETFSAAGEK
                     HFGGDLAGKLVVSGGMGGMGGAQPLAATMAGAAFLGIDVDPERIKKRLKTGYCDFMVT
                     TLDEALRILKNAVRKKENVSVGLVGNCADIIPELAERGVVPDILTDQTSAHDPLNGYV
                     PNGMPLSQAVELRKNDPKAYQEKSLDAIARHVEGMLRLQKMGSVTFDYGNNIRTFAFQ
                     RGVKNAYDFPGFVPAYIRPL"
     gene            complement(1159..1629)
                     /locus_tag="VEJ00_15270"
     CDS             complement(1159..1629)
                     /locus_tag="VEJ00_15270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXX02575.1"
                     /translation="MFRSLNKFDRRGQGSGGVRALQGASRAGIAGTEGFTLLELMIVI
                     SIIMILMAVAVPIYNQSIIQARESVLRSDLNTLRNVIQQYTLDKQKAPQSLDDLKTAG
                     YLREIPMDPMTRQANWEVVQEDVTMAVDQQEPGITDVHSASTATASDGTAYNTW"
     gene            complement(1630..2097)
                     /locus_tag="VEJ00_15275"
     CDS             complement(1630..2097)
                     /locus_tag="VEJ00_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXX02576.1"
                     /translation="MFQHERSAKSEHGFTLLELIVAATILSILTLMAVPLAQVTIKRE
                     KEKQLRQALWQMRDAIDRYKDAADRGGFQIKVDTQGYPPDLDTLVKGVDVQGKKVRFL
                     RSIPNDPMTNSKEWGLRSMQDDPDSDSWGGQNVFDVYTKAEGTGLDGTKYKDW"
     gene            complement(2157..4439)
                     /locus_tag="VEJ00_15280"
     CDS             complement(2157..4439)
                     /locus_tag="VEJ00_15280"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HXX02577.1"
                     /translation="MRRLMRPAVLLLLVAIVTLPAIADKAKSFYDKGRDAEARQNYEA
                     AYDFFKQAYDLKPKDLRYRTALDRTRFEAAASIVHRGQQLRDEGKLEEAATEFQKALA
                     IDPSLFIAKQELNRTLKMINDQKNPPPQAAGPPATLERKVQEAAGPVELAPISNVPIT
                     VKLTDKSDTVYRTIGQLAGINVLLDPDYTARPIKVELNGVTLEEALEITALESKTFWR
                     PVTSNTIFVAQDTPAKRKELEQSVLKTFYLSNLSQPTELQDVVNAIRAVLDVQRVQQL
                     LSQNALVVRGTPDQIALAQKLVEDLDKARPEVIVDIDVMQISKDRSRTLGLSPPTSAS
                     VQLQDNLNTTTSTATSGVTTTPTVGSGNGGINLNSLANLNATNFQVTIPSANLSAVMN
                     NSDTKLIQSPQIRALDNQKASLKIGERLPVATGSFQPGIGGVGINPLVNTQFQYIDVG
                     VNIDVTPHVHADRDVTLKISMEISSEIGQASIGGITQPIIGQKKVDLGEIRLRDGETS
                     LIGGIMDDTQTKALSGIPGLAQIPILRYLFGQTTQDHSEDETVFAITPHIIRAADVNE
                     LNQRAIDIGTATTIELRHVSKPAAAPANAAAPAGQAAAPTPTTQPPAVQQAAASTGGT
                     ASFLFDPPTIQAAKGNTFMVNLLITGAQNVYSVPVQVNYDPKLLQLVNVSNGGFLSQD
                     GQAVALVHREDEAVGQVQITATRPPGAGGVSGQGAVVTMTFQAKTEGQTPLTVTRGGA
                     RDPGMQPITVNGTQASVTVQ"
     gene            4710..5240
                     /gene="smpB"
                     /locus_tag="VEJ00_15285"
     CDS             4710..5240
                     /gene="smpB"
                     /locus_tag="VEJ00_15285"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXX02578.1"
                     /translation="MARQSTHQTKPNPEKSPRRDPTAAGQRDAAVNRIASHNYFLLEK
                     FEAGIALTGTEVKSIRGGRANLKDSYGLVKDGELWLLNCHISPYQHGSYSNHAALRTR
                     KLLMHHDEIRKLMGKTQQKGLTLVPTRMYFKNGRVKVELALAKGKQLWDKRETERRRT
                     ADKEAREAITRGRNRK"
     gene            5284..5874
                     /locus_tag="VEJ00_15290"
     CDS             5284..5874
                     /locus_tag="VEJ00_15290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HXX02579.1"
                     /translation="MKSVCVFCGSSSGNDRRYEEAARDTGCALARAGVRLVYGGGRVG
                     LMGVTADAVLSAGGQVTGVIPRALFKREIAHSGVADMRVVESMHERKELMASLSDGFI
                     ALPGGAGTLEEIFEQWTWSQLGIHAKPCGFLNVNGYFSPLLEMIERMVEEGFLARSFA
                     SMLAIETDPDKLLHRFRAYQPPTNKWSMQGGPAVQA"
     gene            5871..>6079
                     /locus_tag="VEJ00_15295"
     CDS             5871..>6079
                     /locus_tag="VEJ00_15295"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXX02580.1"
                     /translation="MTASPASPPLPPIRIAAAIICDGEGRVLLVRKRDTNFFMQPGGK
                     LDDRETSLAALARELREELGCALKAA"
BASE COUNT         1145 a         1826 c         1817 g         1291 t
ORIGIN      
        1 acagcggacg aatgtaggcg ggtacgaatc cgggaaaatc gtaagcgttc ttcacgccgc
       61 gctggaaagc aaaggtacga atgttgttac cgtaatcgaa cgtcaccgag cccattttct
      121 gcagccgcag catgccttcc acatgacggg cgatggcatc gagcgacttc tcctgatagg
      181 ccttcgggtc gtttttccgt aactcgacag cctgcgagag gggcatgccg ttgggcacgt
      241 atccgttcag cggatcgtgc gcggaagtct ggtcggtcaa aatgtcagga acgacgccgc
      301 gctcggccag ttcgggaatg atgtcagcac aattgcccac cagcccgacc gagacgttct
      361 ccttcttgcg cacggcgttc ttcaggatgc gcaacgcctc atcgagcgtc gtaaccatga
      421 agtcacagta gccggtcttc aggcgtttct tgatgcgctc gggatcgacg tcaatgccga
      481 gaaacgccgc gccagccatg gtggccgcca gcggttgggc tccgcccatg ccgcccatac
      541 cgccggaaac gaccaatttg cccgcgagat cgccaccaaa atgtttttcg cccgcagccg
      601 aaaaagtctc aaacgtcccc tcgacgattc cctgcgagcc gatgtaaatc cacgaaccgg
      661 cggtcatctg gccgtacatc atcaggccgg cgcgctccag ttcgttgaac ttctcccagt
      721 tcgaccagtg gccgacgagg ttcgaattcg cgatcagcac ccgcggcgca tgctcgtgcg
      781 tcttgaaaac gccgacgggc ttgcccgact gcaccagcag ggtctcgtcg ttttcgagat
      841 tcttcagggt tgcgacaatt gcgtgatacg cctcccaact ccgcgccgca cgaccggtcc
      901 cgccatagac gacaaggtcc tgcgggcgct cggcgacctc ttcgtcgagg ttgttcatga
      961 gcatgcgcat ggcagcttcc tgctgccagc ctttgcaggt gatggagttg ccgcggggcg
     1021 cgcggatgga cacaggagct tggatttcgg tttcaaccgg catgaattaa tggtagtgct
     1081 tgaggtgcgg caggcgcaat gcgcccccgc cacgggcaga tgggaacagg attttaggcg
     1141 aaagaagtcg ggtcagatct accaggtgtt gtaggccgtt ccgtcgctgg cggtggcagt
     1201 cgaggccgaa tgcacgtccg tgatcccggg ttcctgctga tcgaccgcca tggtcacgtc
     1261 ttcctgcacg acttcccagt tggcctggcg cgtcatcgga tccatcggga tttcgcgcaa
     1321 gtagcccgcg gttttcaggt cgtcaagcga ttgcggggct ttctgtttgt cgagagtgta
     1381 ttgctggatg acgttgcgca gagtattcag gtccgaccgc agcaccgatt cccgcgcctg
     1441 aatgatcgac tggttgtaga tcggaaccgc caccgccatc aggatcatga tgatgctgat
     1501 cacgatcatc aactccagca gagtgaagcc ctcggtccct gcgatgccgg cgcgagaagc
     1561 cccctgcaac gcccgcactc cgccagatcc ctgtccccga cgatcgaact tgttcaagct
     1621 cctgaacatc taccagtcct tgtatttcgt accgtccagc ccggtgcctt ccgccttggt
     1681 atagacgtcg aacacgtttt gtccgcccca ggagtcggaa tcgggatcat cctgcatcga
     1741 ccgcagtccc cactccttgc tgttcgtcat ggggtcgtta ggaatagaac gcaggaagcg
     1801 gactttcttg ccctgcacgt cgacgccttt caccagagta tcgagatcgg ggggataacc
     1861 ctgcgtgtcc accttgatct ggaagccgcc gcggtcggcg gcgtccttat agcggtcgat
     1921 ggcgtcgcgc atttgccaaa gggcctgccg caattgcttt tccttctcgc gcttgatcgt
     1981 gacttgcgcc agcggcaccg ccatcagcgt gaggatcgac aggatcgtcg ccgccacgat
     2041 gagctcgagg agcgtaaagc catgctcgga ctttgcgctt cgctcgtgtt ggaacatccg
     2101 catcatcatc tgctctatcg atcaaccatc gaggcgaaga actctcccgt ccggctctac
     2161 tgcacggtca ctgacgcctg cgtgccgttg accgtgatgg gctgcattcc ggggtcgcgc
     2221 gcgccgccgc gtgtgaccgt cagcggggtt tgtccctcgg ttttcgcctg gaacgtcatc
     2281 gtgacgacgg cgccctggcc ggaaactccg ccggctcccg gaggccgtgt ggctgtaatc
     2341 tgcacctgtc caaccgcctc atcctcgcgg tgtaccaggg ccacggcttg cccatcctgc
     2401 gagaggaagc cgccattgga aacgttcacc agttgcagca atttcggatc gtagttcacc
     2461 tgcaccggca cggaatacac gttctgcgcg ccggtgatca gcaggttcac catgaaggtg
     2521 tttcccttgg ccgcctgaat cgtcggcgga tcgaacagga aactggcagt cccgcccgtg
     2581 ctggcagcgg cctgctgaac ggccggaggc tgggtcgtgg gagttggagc cgccgcctgt
     2641 cccgcaggag ccgcggcgtt tgccggggcc gcagccggtt tgctgacgtg ccgcaactcg
     2701 atcgtcgtcg ctgtgccgat gtcaatcgcg cgctggttca gctcgtttac atcggctgcc
     2761 cggatgatgt gaggtgtaat ggcaaaaacc gtctcatcct cggaatgatc ctgggtggtt
     2821 tgcccgaaca aatacctcag aatcggaatc tgcgccagcc ccggaatgcc actcaaagcc
     2881 ttggtctggg tatcgtccat gattccgccg atgaggctgg tctcgccatc tctcaagcgg
     2941 atttcgccca ggtcgacctt cttctgtccg atgatgggct gagtgatgcc gccgatgctg
     3001 gcctgcccga tctcggacga gatttccatg gagatcttca gtgtcacgtc ccggtctgca
     3061 tggacgtgtg gcgtcacgtc aatgttcacg cctacgtcga tgtattgaaa ctgcgtattg
     3121 accagcgggt taatgccgac gccgccgatg cccggctgaa acgaaccggt agccaccggt
     3181 aaccgctcgc cgatcttgag agaagccttc tgattgtcga gcgcgcggat ctggggactc
     3241 tgaatcagct tggtatcgct gttgttcatc accgccgaca ggttggccga cggaatcgta
     3301 acctggaagt tcgtcgcgtt cagattcgcc agactgttga ggttaattcc gccattgccc
     3361 gagccgacgg tcggcgtagt cgtgacccca ctggtggcgg tcgatgttgt tgtgttgagg
     3421 ttgtcctgca gctggaccga ggcgctggtc ggcggactca ggcccagcgt ccgcgaacgg
     3481 tccttgctga tctgcatgac atcgatgtcg acgatcacct ccggacgcgc cttatcgagg
     3541 tcctctacca gcttctgcgc cagcgcgatc tggtcgggag ttccgcgcac caccagcgcg
     3601 ttctgggaaa gcagctgctg cacgcgctgc acgtccagca cggcgcgaat ggcattcacc
     3661 acgtcctgca gttccgtcgg ttgcgaaagg ttcgagaggt aaaaagtttt gaggacgctc
     3721 tgctccagct ccttgcgctt cgccggcgtg tcctgggcta cgaaaatggt gttggaagtg
     3781 accggccgcc agaacgtttt cgattccagt gccgtgatct cgagcgcttc ttccagcgtc
     3841 acaccgttga gttccacctt gatcggtctg gctgtgtaat cgggatccag cagcacgttg
     3901 atcccggcta gctgtccgat cgtgcggtac acggtatcgg atttgtcggt gagtttaact
     3961 gtgatgggaa cgttggaaat cggcgccaac tcgactggac cagccgcttc ctgcaccttg
     4021 cgctccaggg tggcaggagg ccccgccgcc tgcggaggcg ggttcttctg gtcgttgatc
     4081 atcttcagcg tgcgattcag ttcctgcttg gcaatgaaca gcgacggatc tatcgccagc
     4141 gctttctgga attcggtagc tgcttcctcg agttttccct catcgcggag ttgctgcccg
     4201 cgatgaacga tagaggccgc agcttcgaac cgggtccggt ccagagcagt ccggtagcgc
     4261 agatccttgg gcttcaggtc gtaagcctgc ttgaagaagt cgtaggccgc ctcatagttc
     4321 tggcgggcct cggcatcacg gcctttgtcg tagaaactct tggctttgtc agcgatggcg
     4381 gggagggtga ctatcgcgac cagcaggagc aataccgccg ggcgcatcag gcgtctcatt
     4441 tcgatgatcc tcacgagagg tgatcacttc cagcttcagt tgttcgccac accagcggtc
     4501 tttccaagcc tgcgccgcgc tgtgcgggcg ttcttggcgg gggggatccg ctcgacacag
     4561 gaacgagatt acgtcccacg cgattataac attgcccttt gtcactgccg aagtgcttgt
     4621 atccgaggca gttatactct ttggttctag gaccaacggc ctacttcagc ggaccccccg
     4681 aagggccgct gctaagatga atcgaaccca tggcgcggca atccacccat caaaccaagc
     4741 ccaacccgga gaaatccccg cgccgggatc ctaccgcagc cggacagagg gacgccgccg
     4801 tcaataggat cgcttcgcat aactatttct tgctggaaaa atttgaggcc ggcattgccc
     4861 tgaccggtac cgaggtcaag tccatccggg gcggaagggc caacctcaag gattcgtacg
     4921 gtttggtcaa ggacggggaa ctgtggctgc tcaattgtca catcagcccc taccagcatg
     4981 gcagctactc caaccacgct gccctgcgca cccgcaagct gctcatgcac cacgatgaaa
     5041 tccgcaagct catgggcaag acacaacaaa agggtctcac cctggttccg acccgcatgt
     5101 atttcaaaaa tggcagagtg aaagttgagc tcgccctggc taaaggcaag cagttgtggg
     5161 acaagcgcga gacggagcgc cgccgtaccg ccgacaagga agcgcgcgag gcgatcacgc
     5221 ggggaagaaa ccggaaatag gtccgcgtcc acaggcctct cgggcattac aatcccgcct
     5281 tgcatgaaat cggtatgcgt attctgcggc tccagctccg gcaacgatcg gagatacgag
     5341 gaagccgcgc gcgacaccgg ctgtgcactg gctcgggccg gcgttcgtct cgtctatggg
     5401 ggtgggcgag tcgggttgat gggcgtgacc gccgatgcag tattgtctgc cggcggccag
     5461 gtcaccgggg tgattccgcg tgcgctattc aaacgggaaa ttgcccacag tggcgtcgct
     5521 gacatgcgcg tggtcgagag catgcacgag cgcaaggaac tcatggccag cctttccgat
     5581 ggattcattg cgctgcccgg cggagcgggg actctggaag agatattcga gcagtggacg
     5641 tggtcgcaac tcggcatcca tgcgaagcct tgcggcttcc tgaatgtaaa cggctacttc
     5701 agtccccttt tggaaatgat cgagcggatg gtcgaagagg ggttcctggc gcgatcgttt
     5761 gcgtccatgc ttgccatcga aaccgatcct gacaagctgc tgcatcgctt tcgtgcctat
     5821 caacccccga cgaacaaatg gtcgatgcaa ggtggtccgg cggttcaagc atgactgcat
     5881 cccctgcgag tcctcccttg ccgccgattc gcatcgcagc ggcgatcatc tgcgatggcg
     5941 aaggccgtgt cctgctggta agaaagcgcg atacaaactt cttcatgcaa ccgggaggaa
     6001 agctcgatga tcgcgaaaca tcattggctg cgctggctcg cgaacttcga gaagagctcg
     6061 gatgtgcgct gaaggcggc
//
LOCUS       DASPUL010000664         4678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1084518, whole genome shotgun sequence.
ACCESSION   DASPUL010000664 DASPUL010000000
VERSION     DASPUL010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084518"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..508)
                     /locus_tag="VEJ00_15300"
     CDS             complement(152..508)
                     /locus_tag="VEJ00_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXX02581.1"
                     /translation="MPQNYVPIFIFILIVGTLLPITLLLAKLVRPENPNKTKLMPYEC
                     GIDPIDNARGRYTVRYYIIAILFVVFDVETIFLFPWAVKFKAFGLFGLFEILIFLAIL
                     IVGYIWVWKKGALEWV"
     gene            complement(658..2970)
                     /locus_tag="VEJ00_15305"
     CDS             complement(658..2970)
                     /locus_tag="VEJ00_15305"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXX02582.1"
                     /translation="MIAEEKITDAEVKTAERTFIENVSRRNFLQGMLSASAFVLWVGK
                     SQLLAKVVTVPDANLALASPGVETAAFHPGVFLGIQADGTVLIVAHRTEMGNGVRTSL
                     PRIVADELDADWSRVKVIQGDGDERYGSQDTDGSHSVREFFEVLREAGATARLMLVRA
                     AALQWGVPEAQCVADPVHVVSDRNSSRKLGYGELASIAATLPVPKKEELRLKTPQEWR
                     YIGKPAPGYDVTEVCSGKPLYGMDVRVDGMLYAAIAHPPVLGGKVKSVDDSAALKVMG
                     VKKTIPIDPFTPPHAFQPLGGVAVIADNTWSAFKGRNRLKIEWDNGGHAVYNSEEYKK
                     QLRETARQPGKVVHAVGDPDAEFAKGGKIVEAEYFAPHLAHASMEPPVAVADVRDDKI
                     TVWSPTQDPQGVQQQVAKALGLKKEDVTCHVTFLGGGFGRKSKPDYAVEAALLSKKTG
                     RPVKVVWSREDDIKFDYFHSVAAMYIKAAVDDKGMPKAWLQRSVFPPIGSTFDKDAVY
                     GGAGEMSLGWNVIPFDVPNFRSENGPATAHVRIGWLRSVANIYHAFAVQSFANELAVA
                     ANRDPYEYLLALIGSGKTIDAGQNPEMAKKYPYDTARLRHVAELAAGKANWGKKKLGK
                     GSGMGIAVHRSFLTYVASVVEVEVDDSGAIRIPQVTTAVDAGIVANPDMVQSQFQGAA
                     VFGTSLARTGEITATGGVIDQSNFHDYPVARMNDAPYHVDVHIVESTAPPAGVGEPGV
                     PPIAPAICNAIYAATGKRVRELPLTRNGFA"
     gene            complement(2967..3422)
                     /locus_tag="VEJ00_15310"
     CDS             complement(2967..3422)
                     /locus_tag="VEJ00_15310"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXX02583.1"
                     /translation="MIRLKVNGVAQSFDGDPEMPLLWYLRDVLGLTGTKFGCGMALCG
                     ACTVLQNGKAIRSCQKQMGAAEGLEITTIEGISAHGSHAVQTAWATVNVPQCGYCQPG
                     QIMQAVSLLSSKPQPSDSDIDDVMSGNICRCGTYQRIRAAIKTAAKEVA"
     gene            complement(3493..4251)
                     /locus_tag="VEJ00_15315"
     CDS             complement(3493..4251)
                     /locus_tag="VEJ00_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02584.1"
                     /translation="MIAKMTALLIAVLLSVSCMEGQEMRSGEALLRAMHDRYQSSWYQ
                     TITFTQKSTTYKPDGTSSVETWYEAARLPGKLRIDIGPAADGNGVVLADGEVTVFRGG
                     KTTATRHSLNMLLVLGFDVYRQDAATTIQVVQAEGYDLSKIREDSWEGHPAYVVGADK
                     GDLKSKQFWIAKDTLLFMRIIEPAREDPAKLDDVRFTDYRPLAGAWVAARVEVYSEGK
                     LVFSEDYSDIRANVRIEPAVFDPQRYNTTHWEKP"
     gene            complement(4379..>4678)
                     /locus_tag="VEJ00_15320"
     CDS             complement(4379..>4678)
                     /locus_tag="VEJ00_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02585.1"
                     /translation="AYLSEYPVLEALDRFCRALKRFATARGKSQLYHETITHAYFFLI
                     RERMARCASATWDEFARCNSDLLTWKGGILTRYYHDATLQSDLARAVFLFPDKCA"
BASE COUNT          918 a         1396 c         1418 g          946 t
ORIGIN      
        1 gtctcggcag gggaggaatc gatggggttt gcgggggaag cggtcatttc agttgaatgc
       61 gtacggaaga gaacgaccac gtgggctaaa gcccagtgaa taccggaaca ctaacgcggc
      121 cctgaagggc cgctcttcca caatttaagt tctacaccca ctccagcgcg ccttttttcc
      181 atacccagat gtagcccacg atcaggatgg ccaggaagat gagaatttcg aacagcccga
      241 acaggccgaa agccttgaac ttcaccgccc acggaaatag aaaaatcgtc tccacatcga
      301 agacgacgaa taggatagcg atgatgtagt agcgcacggt gtagcgcccg cgggcgttgt
      361 cgatcggatc gatgccgcac tcgtagggca tcagcttcgt cttgttgggg ttctccggac
      421 gcacgagctt ggccagaagc agcgtgatcg gaagaagtgt tcccacgatc aggatgaaga
      481 tgaagatcgg aacgtagttc tgcggcatag agcgactcag ttcagcacgt catcgtagcg
      541 cggatggcct tcggctgcaa ccggcaagcc gagcttcctc caacgtgcgt gtcacacgac
      601 agcaacgccg tcgagctgcg cccgacggac agccgagggc ggctgtccct acattgctca
      661 ggcaaaaccg ttgcgagtca gcggcaactc ccttaccctt ttaccggtcg cggcatagat
      721 cgcattgcat atcgccggcg cgatcggtgg cacgccgggt tcgccgacgc ccgcaggcgg
      781 ggccgtgctc tccacaatat ggacatcgac gtgatagggc gcgtcgttca tgcgggcgac
      841 cggataatcg tggaagtttg actggtcgat cacgccgccg gttgccgtga tttcgccggt
      901 gcgcgcgagg ctggtgccga agacagcggc cccctggaac tgcgactgaa ccatgtccgg
      961 gttagcgaca attcccgcgt cgacggcggt ggtgacctgt ggaatgcgaa tcgcgccgct
     1021 atcgtcgacc tcgacttcca ccacgcttgc tacgtaagtc aggaagctgc ggtgcacggc
     1081 gatgcccatg ccgcttccct ttcccaactt ctttttgccc cagtttgcct tcccggcggc
     1141 gagctcggcg acgtggcgca ggcgtgcggt gtcgtaggga tatttcttcg ccatctccgg
     1201 gttctgacct gcatcaattg tcttcccaga tccgattaag gccagcaggt actcgtacgg
     1261 atcgcggttg gcggcaaccg cgagttcatt ggcaaacgac tgcaccgcga atgcgtggta
     1321 aatgttcgcc accgagcgca gccagccgat gcggacgtga gccgtcgcgg gaccgttctc
     1381 ggaacggaaa ttgggaacat cgaagggaat gacgttccag cccagcgaca tttcgccagc
     1441 tccgccgtag acggcgtcct tgtcaaacgt cgatcctatg ggtggaaaca cggaacgctg
     1501 gagccacgct ttcggcatgc ctttgtcgtc aacggcggcc ttgatgtaca tcgctgccac
     1561 ggagtggaaa tagtcgaact tgatgtcgtc ttcgcggctc cagaccactt tgaccggcct
     1621 gccagtcttc tttgagagca gcgctgcttc aaccgcgtaa tctggctttg atttgcgtcc
     1681 gaaacctcca ccaaggaagg tgacgtggca agtcacatct tccttcttca gaccgagagc
     1741 tttcgccacc tgctgctgca cgccttgcgg atcttgtgtc ggcgaccaca ccgtgatttt
     1801 gtcatcgcgc acgtcggcga ctgccacggg aggctccatg gaagcatgtg cgaggtgcgg
     1861 cgcgaagtat tccgcctcaa cgatcttgcc gcccttggcg aattcggcgt caggatcgcc
     1921 gacggcatgg acgacttttc ccggctggcg ggcagtttcc cgcagctgtt ttttgtattc
     1981 ctcagagtta tagacggcgt ggccgccgtt gtcccattcg atcttcagac ggttgcgccc
     2041 tttgaatgca gaccaggtgt tgtcggcgat gacggctact cctccgagcg gttgaaaggc
     2101 atggggcggc gtgaacggat cgatggggat ggtcttcttc actcccatta ccttcaaagc
     2161 ggcggagtca tcgaccgact taacttttcc tcccagcacg ggcggatgag cgatcgccgc
     2221 ataaagcatg ccgtcgacac ggacgtccat gccgtacagc ggcttacccg agcagacctc
     2281 ggtgacgtcg taaccgggcg ctggcttgcc gatgtagcgc cactcctgcg gcgtcttgag
     2341 ccgcagttct tcttttttcg gaaccggaag cgtggcggca atggaggcca gctcgccgta
     2401 tcccaacttt cgagaagaat tcctgtccga gaccacgtgc acaggatcgg ccacacattg
     2461 cgcttccggg acgccccact gcagtgcggc agctcgaacc aacatcagac gagcggttgc
     2521 gccggcttcg cgcagcacct cgaaaaattc gcggaccgag tgcgagccgt cggtgtcctg
     2581 cgatccatag cgctcgtcgc cgtcgccctg aatgactttg acgcggctcc agtcggcgtc
     2641 cagttcgtcc gccacaatgc gcggcaggct tgtacgaacc ccgttgccca tttccgtgcg
     2701 gtgggccacg atgagcacag tgccgtcggc ctgaattcca agaaacacgc ccggatggaa
     2761 cgccgccgtt tcaacgcccg gactcgccag cgcgaggttg gcatcgggca cggtcacgac
     2821 cttggcgagc agctgagatt tccccaccca cagcacgaat gcactggccg aaagcatgcc
     2881 ttgcaggaag ttccggcgcg acacgttctc gatgaaggtc cgttccgcgg tctttacttc
     2941 ggcgtcagtg atcttttctt cggcgatcat gcgacctcct tagccgcagt cttgatcgcg
     3001 gcccggatgc gctggtacgt gccgcaacgg cagatgttgc cggacatcac atcgtcgatg
     3061 tcgctgtcgc tgggctgagg cttggacgac agcagcgaaa ccgcctgcat gatctggccg
     3121 ggctggcagt acccgcactg cggaacgttt accgtggccc aggcggtctg aacggcatgc
     3181 gagccgtgag cggagatacc ctcgatcgtc gtgatttcca gaccttcggc tgcgcccatc
     3241 tgcttctggc aggagcggat tgccttgccg ttttgcagca cggtgcaggc accgcacagc
     3301 gccatgccgc atccgaattt tgtgccggtg agaccgagga cgtcgcgcag gtaccacaac
     3361 agcggcatct cgggatcgcc gtcgaaagac tgcgcgactc cgttgacctt gaggcggatc
     3421 atgaggcact tcctcgcttg gcccgtggaa tggaaattac ggattcattc tactgctgcg
     3481 aacaggggaa cgttatggtt tttcccaatg agtcgtgttg tagcgctggg gatcgaaaac
     3541 cgcaggctcg atgcgcacgt tggctcgaat gtcagagtag tcttcggaga agacaagctt
     3601 gccttcggaa tagacctcga cgcgagctgc gacccaggcg cccgcgagcg gcctgtagtc
     3661 ggtaaaacgg acgtcgtcga gtttcgcagg atcttcgcgc gccggttcga tgatgcgcat
     3721 gaacaggagc gtgtcctttg caatccagaa ctgcttcgac ttcaaatcgc ccttgtcagc
     3781 tcccacaacg taggcgggat ggccttccca cgagtcctcg cgaattttcg aaagatcgta
     3841 gccctcggct tgcacgacct gaatcgtggt cgcagcatcc tggcggtaaa catcgaagcc
     3901 gagcaccagc agcatgttca gcgaatggcg cgtggctgtg gtcttgccgc cgcggaaaac
     3961 cgtgacttcg ccatccgcca gcactacgcc gttcccgtcg gcggcaggcc caatgtcgat
     4021 gcgcagcttg ccaggcagcc gcgcggcttc gtaccaggtt tcgaccgagc tggtcccgtc
     4081 cggcttgtag gtggtgctct tctgagtaaa ggtgatcgtc tgataccacg aagactgata
     4141 ccggtcatgc atcgcgcgca gcagggcctc gcccgagcgc atttcctgcc cttccatgca
     4201 agaaacagaa agcaggacag cgatcagcag ggccgtcatt ttggcgatca tctgcgcctt
     4261 tctccggggc aaagaggaat tctgtgcgtg ggcgagtccc gttgcgcaca tggcgcgtta
     4321 gacgcagcgg accggcaaaa gacagcagcg ctccaaaagc tgagtctcag ggcgtgcttc
     4381 aggcgcattt gtcaggaaag aggaagacgg cgcgggccag atccgattgc agcgtcgcgt
     4441 cgtggtagta gcgagtcaga attccgccct tccacgtcag gagatcggaa ttgcagcggg
     4501 cgaattcgtc ccaggtggcg ctggcgcagc gggccatgcg ctcgcggatc agaaaaaagt
     4561 aagcgtgcgt gatcgtctca tgataaagct gcgacttgcc gcgggcggtc gcaaagcgtt
     4621 tcaacgcgcg gcaaaatctg tccagagcct cgagaaccgg gtactccgag agataggc
//
LOCUS       DASPUL010000665         5867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1630899, whole genome shotgun sequence.
ACCESSION   DASPUL010000665 DASPUL010000000
VERSION     DASPUL010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630899"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /gene="mnmA"
                     /locus_tag="VEJ00_15325"
     CDS             complement(<1..920)
                     /gene="mnmA"
                     /locus_tag="VEJ00_15325"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXX02586.1"
                     /translation="MSTDTIAVAMSGGVDSSTVVAMLRAEGHNVIGLTMQLWNQRRLV
                     GRGGMPESVEGRCCSLDDVYDARRVAQQIGIPYYVVNHEERFERDVVRPFVEEYLSGR
                     TPIPCSLCNNHLKFDQLLVVARQIGAERVATGHYARVAYDEARGRWLLKRPADVSKDQ
                     TYFLFGLTQDQLSRTLFPLGDMTKPQVRELAHQHGLAIAEKPDSQEICFVPGGDYKRF
                     IDAYLAEQGESLPDTAGELVTTNGEVIGEHTGIHNFTVGQRKGLGVATGSPLYVIQIS
                     GAEKQVIVGGEENLYSRTLRARRVNLISIE"
     gene            complement(1004..1453)
                     /locus_tag="VEJ00_15330"
     CDS             complement(1004..1453)
                     /locus_tag="VEJ00_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02587.1"
                     /translation="MTFRKYLVLAGVTVFAAAGDSMLSHGMKQTGNVSLHHLSSLIAA
                     VLNPWVAVGILLLLAFFAAYMNSLSWADLTYVLPATSLGYVLLALIARVVLHEQVSPL
                     RWLGIALISGGVGFVAGGPALTHHPQAEGSVSAETEAELSRTAAAPK"
     gene            complement(1566..3035)
                     /gene="hpnJ"
                     /locus_tag="VEJ00_15335"
     CDS             complement(1566..3035)
                     /gene="hpnJ"
                     /locus_tag="VEJ00_15335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HXX02588.1"
                     /translation="MPLKTLFLNPPSFENFDGGAGSRWPATREIESYWYPVWLAYPAG
                     MLEGARLLDAPPHHISGEETIQIARDYEFLVLFTSTPGFPGDIRLVRAIKDANPKIKI
                     AFVGPHVSVLPEKSLRDCPQIDFVCRKEFDFAVVDYAKGKPLEEIPGVSFLKNGQVVH
                     NPDAPQIQDLDALPHVTEIYKRDLDVTRYNVPFLLNPYVSLYTTRGCPAQCTFCLWPQ
                     TLSGHPWRKRSTDDVAREMAKAKDFWPNVKEFFFDDDTFNIQKARTIELCAKLKPLGL
                     TWSCTSRVTTDFETLKAMKDAGCRLLIVGYESGDQQILKNIKKGATIERARQFTKDCH
                     KLGLVIHGDFIMGLPGETRETINNTIAFAKELDVETIQVSVAHAYPGTELYDFAVKNG
                     FMVGDNKMVDEGGHQLAHIQYPGLPADEILAAVHRFYDEYYFRPKAVYRILKKAAFDG
                     HERKRLYKEAKTFLKLRAARNKMIKQHAEAGAAAAPAKA"
     gene            complement(3213..4091)
                     /locus_tag="VEJ00_15340"
     CDS             complement(3213..4091)
                     /locus_tag="VEJ00_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02589.1"
                     /translation="MRRLASIAAFGLFLAVPLWAQRGGGHGGGFGGHAGGFSGGHMGG
                     GHAGFTGGHAAVHSFSGMHSAPGVSRSFTSPRSFSRPSSSLRAFNGRGPFLHDRFGNR
                     DRFRDRFRDRGFFRNRGFFNNCWGFGCSLYGYPWWGYDPWLWNDDSSYDQSYEQNLAD
                     AREMDEESLEEQRMLRQEEADGDQDAYAQRAPAPTPAPAPAPAAIQPATVLVFRDQHK
                     QEVQNYAIVGHTLWNFAPQHTQKIALADLDIPATVKANDDRGVTFRVPAASDAPGAPP
                     ASMQGQPPAARSSSSV"
     gene            complement(4148..>5867)
                     /locus_tag="VEJ00_15345"
     CDS             complement(4148..>5867)
                     /locus_tag="VEJ00_15345"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX02590.1"
                     /translation="KDYPDWSNRQFTVPGLPFYMPAVADDAKDSIFLGSPLETISNTR
                     LELPKGYRPQVPTNVELKYDFAEYHAFYSQEPGVLIARRELTIKQREVPVAKLDDYRK
                     FIKDLRNDVDRYVQTSSGGAVASSNPAISGAFPSFLAEVRNLPDSNSAEANRLEADAR
                     SGMPRSNPQDAFDAFKSVVDADPKFARAWIELGMMYLMGQPEKALDALRHAVDADPKQ
                     VVPHKVYAFALSTLRRTDDALQAWRDTLALAPNDRDANAALGGLLTQQKRYSEAVPYA
                     EAYAKADSSPAAQITLGTAYLKAGQNEKGTATLEKVLGEDASPDSLNNAGYALADASL
                     ALPKALEYAQRAVDGQEKESHDVELSNLLPDDLACSQKIGYDWDTLGWAHFRLGHLPQ
                     AEDYLNAAWLLSQFSVNADHLGQVYEQEKKTEKAIHMYRLALVTTEGREDLDATRHRL
                     EHLGGKAPPGPMEVANDHTRDELSQLRTVKLKRLVSGQASAEFFLLFKPGARHPDPEL
                     EDVQFVSGSEKLKAEADALYDANFQVAFPQASSAKLVRRAIVMCSPISGCQAVLYTPG
                     SVHSVN"
BASE COUNT         1138 a         1680 c         1874 g         1175 t
ORIGIN      
        1 ttctcgatgg aaatcagatt tactcgcctg gctcgcagcg tcctcgaata caaattctct
       61 tcgcctccaa cgatcacctg cttctctgcg ccgctgatct ggatgacata gagcggcgag
      121 ccggtggcca cgccgagtcc cttgcgctgg ccgacggtaa agttgtgaat gcccgtgtgc
      181 tcgccgatga cctcgccgtt cgtggtgacg agttcgccgg ctgtgtcggg caagctctcg
      241 ccctgctcgg cgaggtacgc gtcgatgaag cgcttgtagt cgccgccggg gacgaagcag
      301 atttcctgcg agtcgggctt ttcggcgatc gcgaggccgt gctggtgggc gagttcgcgg
      361 acctgcggct tggtcatgtc gccgagcggg aatagagtgc ggctgagctg gtcttgcgtg
      421 aggccgaaga gaaaatacgt ttgatccttg ctcacgtcgg cggggcgctt gagcagccaa
      481 cgccctcgcg cttcgtcata ggcaacacgg gcatagtggc cggtggcgac gcgttcggcg
      541 ccgatttgcc gcgcgacgac caggagttga tcaaacttca agtgattgtt gcagaggctg
      601 caggggatgg gcgtgcgccc ggagagatac tcttcaacga aggggcgcac cacgtcgcgc
      661 tcgaatcgct cttcgtgatt gaccacgtaa tacgggatgc cgatctgctg cgcgacgcgg
      721 cgggcgtcgt agacgtcgtc gagcgagcag cagcgtccct cgacggactc gggcatgcct
      781 ccgcggccga cgaggcggcg ctggttccag agctgcatgg tcaggccgat gacgttgtgt
      841 ccctcggcgc gcagcatggc cacgacggtg gaagaatcca caccgcccga catggcgacg
      901 gcaatggtgt cggtggacat cggctcttcg ctcttggcct ttggctattg gctagcccaa
      961 tcacagccta gcaagattct aaacgagtcg aagcagaggc cggtcatttc ggggccgctg
     1021 ccgtacggga aagctcggct tcagtttctg ccgagacgct cccctccgct tgcggatgat
     1081 gggtaagcgc agggccgccg gcgacgaagc caactccgcc ggaaatcagc gcgatgccaa
     1141 gccaacgtag cgggctgacc tgctcgtgca gcacgacgcg ggcgatcagc gcgagcagga
     1201 cgtatcccag cgacgtggca ggcaagacgt aggtcagatc ggcccaggag agcgagttca
     1261 tgtaggcggc aaagaaggcg aggagcagaa gaatgccgac ggcgacccag ggattgagca
     1321 ccgctgctat caggctcgag aggtgatgca gggagacgtt gccggtctgc ttcatgccgt
     1381 gcgagagcat ggagtctccg gccgcggcga agacggtgac tccggccagc acaagatatt
     1441 tgcgaaaggt cacggagaac tcgagtgggc ctgcagggaa aaaactacct caggcgctaa
     1501 agcgcggaca cctttataac gccatcggcg cggctaaagc cgcgcccctt caagacagag
     1561 ccacatcacg ctttcgccgg cgcagccgcg gctcccgcct cggcgtgctg cttgatcatc
     1621 ttgttgcggg cggcgcgcag cttcaggaac gtcttggctt ccttgtacag gcgcttgcgc
     1681 tcatggccgt cgaaagccgc tttcttcagg atgcggtaga cggcttttgg acggaaataa
     1741 tattcgtcat agaagcgatg taccgccgcg aggatctcgt ccgcgggcag gcccggatac
     1801 tgaatgtggg ccagctgatg tccgccttcg tcgaccattt tgttgtcgcc gaccatgaag
     1861 ccgttcttca ccgcaaagtc gtagagttcg gtgccgggat aggcgtgcgc cactgaaacc
     1921 tggatggtct cgacgtcgag ttccttggcg aaggcgatgg tgttattgat agtttcgcgc
     1981 gtctcgccgg gcagacccat gatgaagtcg ccgtgaatca ccaggccgag cttgtggcaa
     2041 tccttggtga actggcgggc gcgctcgatg gtggcgccct tcttgatgtt cttcaggatt
     2101 tgttgatcgc cggattcgta gccgacaatg agcaggcggc agcccgcgtc cttcatggct
     2161 ttgagtgttt cgaaatcggt ggtgacgcgc gacgtgcacg accaggtcaa gccgagcggc
     2221 ttcagcttcg cgcagagctc gatggtgcgc gctttctgaa tgttgaaggt gtcgtcatcg
     2281 aaaaagaatt ccttcacgtt gggccagaag tctttggcct tggccatctc ccgggcaacg
     2341 tcatcggtgg agcgcttgcg ccacggatgt ccgctgagcg tctggggcca caggcagaag
     2401 gtacactgcg ccgggcagcc gcgcgtggtg tagagcgaca cgtacggatt cagaagaaac
     2461 gggacgttgt agcgggtgac gtcgagatcg cgcttgtaga tttcggtgac gtgcggcagg
     2521 gcgtcgagat cctggatctg cggggcgtcg gggttgtgca cgacctggcc gttcttcaga
     2581 aaagacacgc cggggatctc ttcgagcggc ttgcctttgg catagtcaac gacagcgaaa
     2641 tcgaactctt tgcggcaaac gaaatcgatc tgtgggcagt cgcgcaaaga cttttccggc
     2701 agcacgctga cgtgcgggcc cacgaaggcg atcttgatct tcggattggc gtctttgatg
     2761 gcccgcacta ggcggatgtc accggggaag ccaggagtcg atgtgaatag caccaggaac
     2821 tcatagtccc tggcgatctg tatggtttct tcgccggaaa tgtgatgggg cggggcgtcg
     2881 agcagccgcg cgccctcgag cattccggca gggtaggcga gccacaccgg gtaccagtag
     2941 ctttcgatct cgcgggttgc tggccagcga gagccagcgc ctccgtcgaa attctcaaac
     3001 gaaggcggat tcagaaacag agtttttaag ggcatattga gttttcttta attttagcat
     3061 ccgagcgaag atattccccg gaaccgctcg tgttgctcct gattccctat ccaacgtaaa
     3121 tgcttgaatc tactgtacaa gctcagccac aaaaagcaaa tcacgacacc ttcaagatgt
     3181 tcgtgaggag cgtgaacggg gaaaaccggg gcttaaacgg atgaactgct gcgtgcggct
     3241 ggcggctgtc cttgcatact tgccggaggt gcgccgggcg catcgctcgc cgccggaacg
     3301 cggaaggtga ctccgcgatc gtcgttggcc ttgacggtgg ccgggatgtc caggtccgcc
     3361 agcgcgatct tctgcgtatg ctggggagca aaattccaga gcgtgtggcc gacgatggcg
     3421 tagttctgga cttcctgctt gtgctggtcg cggaagacga ggacggtggc gggctggatc
     3481 gctgcaggcg cgggagctgg agcgggtgtg ggagccggcg cgcgctgcgc gtaagcgtcc
     3541 tgatcgccgt cagcttcttc ctgtcgcagc atccgctgct cttcgagact ttcctcgtcc
     3601 atctcgcgag catcggctaa gttttgctcg tagctctggt cgtatgagga atcgtcattc
     3661 cacagccagg gatcgtatcc ccaccaaggg tatccgtaga gcgagcaccc aaacccccag
     3721 cagttgttga agaacccccg attgcggaag aacccacggt cgcgaaaacg atccctgaat
     3781 cggtctcgat tgccgaagcg gtcgtgcagg aacggccccc ggccgttgaa agcgcgcaac
     3841 gaggagctgg gacgcgagaa ggagcggggt gatgtaaagc tgcgggaaac gccgggagcg
     3901 ctgtgcattc cgctgaaaga gtgcactgct gcgtggcctc cagtgaaccc ggcgtggcct
     3961 ccacccatat gcccgccgct gaaaccaccg gcatggccac cgaagccacc cccgtgccca
     4021 cctccgcgct gcgcccacaa aggtacagcc aagaacagcc cgaacgccgc gatggaagcc
     4081 aggcgacgca tacacctaaa ttataagcct tgcgggcaag aaaggttgcc tggtgccgtg
     4141 ccgcaattca attcacggaa tgaaccgacc cgggagtgta aagcaccgcc tggcagcccg
     4201 aaatcggcga acacataaca atcgcgcggc gcaccagctt ggccgagctg gcctggggaa
     4261 aagccacctg gaaatttgcg tcgtagaggg catcggcctc cgctttgagt ttctcggatc
     4321 cgctgacgaa ctgcacgtct tcgagttctg ggtcgggatg cctggctccc ggcttaaaaa
     4381 gcaggaaaaa ctcggcgctg gcttgccccg aaaccaggcg cttgagcttg acggtgcgca
     4441 gctgactcag ttcgtctctc gtatggtcgt tggcgacctc catgggaccc ggcggcgctt
     4501 tccctcccaa atgttcgagg cgatggcggg tcgcgtcgag gtcttcgcgc ccttcagtag
     4561 tgacaagagc caagcggtac atgtgaatcg ctttctcggt cttcttttcc tgctcgtaaa
     4621 cttggcccag atggtcggca ttgacgctga actgcgacaa caaccatgcg gcgttcaggt
     4681 aatcttcggc ctgcggcaga tggccgaggc gaaagtgcgc ccagcccaaa gtgtcccagt
     4741 cgtagccgat cttctggctg caggccagat cgtcaggaag cagattggag agctccacgt
     4801 cgtgcgattc cttttcctgt ccatcgacgg cgcgctgcgc gtattccagc gccttgggca
     4861 gcgcgaggct ggcatcggcc agggcatagc cggcattgtt caacgaatcc gggcttgcat
     4921 cctctcccag gactttctcc aaagtagcgg ttcccttttc attttgaccc gccttcaggt
     4981 aagcagtccc cagggttatc tgggccgccg gagaggagtc cgccttggcg taagcctccg
     5041 catagggaac ggcctcggaa tagcgctttt gctgggtaag cagtccacca agagcggcgt
     5101 tggcgtcacg atcgttgggt gcgagcgcaa gcgtgtcccg ccaggcttgc agagcgtcat
     5161 ccgtgcggcg cagggtgctc agcgcaaagg catagacctt gtgcgggacc acctgcttgg
     5221 gatcggcgtc gacggcgtgg cgcaaggcgt caagcgcctt ctcgggctgc cccatcagat
     5281 acatcatccc taactcgatc cacgcgcggg cgaattttgg atcggcgtcc acgacacttt
     5341 tgaacgcgtc gaaggcgtct tgcgggttgg agcgcggcat cccgctgcga gcatcggctt
     5401 ccagccggtt ggcctcggcg gagttgctgt cgggtagatt ccgaacctct gccaggaagg
     5461 aggggaacgc accggatatc gccggattcg aggaggcaac cgcaccgccg gacgaagtct
     5521 gtacatagcg atcgacatca ttccgcagat ctttgatgaa tttgcggtag tcgtccaact
     5581 tggctaccgg aacttcccgc tgcttgatgg tcagctcgcg gcgtgcgatc agaacacccg
     5641 gttcctgcga atagaaggcg tggtattccg cgaaatcgta tttcaactct acgttggtcg
     5701 gaacttgcgg ccgataacct ttgggtagct ccaagcgtgt gttcgaaatc gtctctaacg
     5761 gcgatcccag aaaaatcgaa tccttggcgt catcggcgac cgccggcatg taaaacggaa
     5821 ggccgggaac ggtgaactgg cgattggacc aatccggata gtcttta
//
LOCUS       DASPUL010000666         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_403530, whole genome shotgun sequence.
ACCESSION   DASPUL010000666 DASPUL010000000
VERSION     DASPUL010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403530"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VEJ00_15350"
     CDS             complement(<1..354)
                     /locus_tag="VEJ00_15350"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX02591.1"
                     /translation="MNSLVQDLRYAVRQLRKSPGFTAVAVLTLALGIGANTAIFSVVN
                     AVLLNPLPFSNPDRLVSMFEENEHFSNYSISYPNFLDWQRDNHAFQAVAAYRWADGAI
                     TGLGAAEDVPARHVSA"
     gene            complement(364..2790)
                     /locus_tag="VEJ00_15355"
     CDS             complement(364..2790)
                     /locus_tag="VEJ00_15355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXX02592.1"
                     /translation="MNSWIQDLRYGVRQLRKSPGFTCSAVLILAIGIGGATAIFSALN
                     PILFQPLPYPHAARIMMIWYADESGERSPLAFHAYREVLERNRSFESLAVMKVWQPTL
                     NGANLPERLAGQQVSAAYFRTLGVNPVLGRDFQPSDDVRNGPQVVILSDGLWRRRFGG
                     DRTIVGSTIRLDDQIYTVIGILPPGFENVLAPDAEVWSPLQYDSGNIADFQTREWGHH
                     LRMVGRLRSGVGRDQARNDLAAIAATPVTEFPRPRGSTMGHGLTIDALQEDVTRGVKP
                     ALLAVFAAVGLVLLIACVNVTNLMLACSAQRQGEIAMRAALGAAAPTLARQMVTESLL
                     LATVGGVLGMVVAQAGIRVLVALSPAGLPRVNAIRLDGFAFAFAFCVTGLVGIAVGLI
                     PAWQASRGDLLGALRQASQRTTGQHQVARRAFVVAEVALALVLLVGAGLLLHSLRLLL
                     AVPSGFDTSGMLSMQVQTYGRKYDDDRVCHQFFAQALDAVRQVPGVSEAAFTSQLPLS
                     GDSDAYGAFFEGDGPEIGYSVYRYAVTPGYLEALRIPLRRGRLLDEHDTTGMPPVMLL
                     SESLAKRKFPNQDPIGKRVNVSGTAGSPLFTVVGVVGDVKQESLALDQSDAVYTTTTQ
                     WHWADGTLSLVVRARGDAAALVPALKNAIWSVDKDQPIVRVALMDDLLAASAAERRFV
                     LILFEAFGLVALVLAATGLYGVLAGSVTERTRELGLRAALGASRGRIVSLVLRQGMIL
                     TAVGVGLGLAGAAAAGHVISTLLFGISRLDPLTYAVVIILLAAVSAMACAIPAWRAAR
                     VDPMVALRYE"
     gene            complement(2787..>3338)
                     /locus_tag="VEJ00_15360"
     CDS             complement(2787..>3338)
                     /locus_tag="VEJ00_15360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXX02593.1"
                     /translation="GLLDTPTSGRYLLNSKAVENLDFAERARIRNQEIGFIFQSFNLI
                     GDLTVYENVELPLTYRQRMPSAERKKRVMEALERVGMAHRVRHYPSQLSGGQQQRVAV
                     ARALGGKPSILLADEPTGNLDSRNGEAVMELLADLHRDGATICMVTHDPRFAKHAQRE
                     VHLFDGKVVAEDELDKLMQDANA"
BASE COUNT          716 a         1095 c          956 g          571 t
ORIGIN      
        1 ggctgatacg tgtcgagcgg gtacgtcttc cgctgccccc agacccgtga tggcgccatc
       61 cgcccagcgg tatgcggcca cagcctggaa cgcgtgattg tcacgttgcc aatccagaaa
      121 gttgggatag gaaatcgagt aattggagaa atgctcattc tcctcgaaca tcgaaacaag
      181 gcggtcggga ttcgaaaatg gaagcgggtt gagcaagacc gcgttcacga ccgagaaaat
      241 tgccgtgttc gcacctatgc ccagcgccag cgtcagtacg gcaactgcag tgaatcccgg
      301 gctcttgcgc aactggcgca ccgcgtaccg aagatcctgg acgaggctat tcatgggaga
      361 ctcctattcg taccgcagcg ccaccatggg atcgaccctc gccgcccgcc atgcggggat
      421 ggcgcaggcc atcgccgaca ctgcagcgag caaaatgatc acgactgcat aggtaagagg
      481 atcgagtctg gaaattccga acagcaaggt ggagatgacg tgacccgcag ccgctgcccc
      541 cgccaagcca agtcccacac caaccgcggt gagaatcatt ccctggcgga ggaccagact
      601 cacgatgcgt ccgcgtgagg cacccagcgc ggctcgcaac ccgagttcgc gcgtacgctc
      661 agtaacgctg cccgccagaa ctccgtaaag accggttgcc gccagcacca aagcaaccaa
      721 tccaaacgcc tcgaacagaa tcaggacaaa gcgccgctcg gctgccgacg cagccagcag
      781 gtcatccatc agcgcaaccc gcacgatggg ttgatctttg tcgacggacc agattgcatt
      841 cttgagggcc ggaaccagag cagccgcatc cccgcgcgcc cgcaccacca gcgaaagcgt
      901 cccgtccgcc caatgccact gtgtagtggt cgtgtagacc gcgtccgatt ggtccagcgc
      961 cagcgactcc tgcttcacgt cgcccaccac gcccacgacc gtaaacaatg gcgaacccgc
     1021 tgtgccactc acgttgacac gcttgccaat cggatcctga ttgggaaatt tgcgcttggc
     1081 cagcgactcg ctgagcaaca ttaccggagg catgccggtg gtatcgtgct catcgagtaa
     1141 acgcccgcgc ctcagtggga tgcgcagcgc ttcgaggtag cccggtgtca ccgcgtagcg
     1201 ataaacggaa tatccgatct cgggtccgtc tccttcgaag aaagcgccgt acgcgtcgga
     1261 atccccgctc aacggcaact ggctggtaaa ggccgcttcg ctgacgccgg gaacttggcg
     1321 caccgcgtca agcgcttgcg caaagaactg atgacaaaca cggtcgtcgt catatttccg
     1381 cccataagtc tgcacctgca tgctcagcat gccggaagta tcgaaaccag agggcacggc
     1441 aagaagaagg cgcagactgt gcagcagcaa tcctgcgccg accagcagaa ccagagctag
     1501 cgccacctcg gccacgacga atgcccgccg cgcaacctga tgttgcccgg tggtccgctg
     1561 cgaagcctgc cgcagcgcgc ccagcaggtc gccccgggag gcttgccagg cgggaatcag
     1621 acctactgcg attccaacca gacccgttac gcagaaggca aaggcgaatg caaacccgtc
     1681 caggcggatc gcattcactc gcggcaatcc ggcagggctc aatgccacca gcacccgaat
     1741 accagcctgc gccaccacca ttcccaatac cccgcccacg gttgccagca gcaggctttc
     1801 ggtgaccatc tgcctggcca gcgtcggcgc cgccgcaccc aaagcggcgc gcatggcgat
     1861 ctccccctgc cgttgggcgc tacacgctaa catcagattg gtcacgttca cacaggcaat
     1921 caacagcacc aagccaacag cggcaaaaac tgccagaagg gctggcttta cgccgcgcgt
     1981 tacgtcctct tgcagcgcat cgatcgtcag cccgtgcccc atcgttgacc cgcgtggccg
     2041 gggaaactcc gtcaccgggg tcgcggcgat cgccgccaaa tcgttcctcg cctggtcgcg
     2101 cccaacgccg gagcgcaatc gccccaccat acgcaaatga tgtccccact cgcgggtctg
     2161 gaaatcggca atattgccgg agtcgtactg taacggcgac caaacttcag catcgggagc
     2221 caacacgttc tcgaagcccg gaggcaatat cccgatcacg gtatagattt gatcgtcaag
     2281 ccggatcgtg ctccccacga tcgtcctatc gccgccgaag cgcctgcgcc acagaccatc
     2341 gctgagaatt acgacctggg gaccgttgcg tacgtcgtcg gacggctgga agtccctgcc
     2401 tagcaccgga ttcacaccca gcgtgcggaa ataggcagcg ctcacctgct gtccggcgag
     2461 gcgttccggc agattggcac cgttgagggt gggctgccac accttcatca ctgcaagcga
     2521 ttcgaacgat cgattgcgct ctaacacttc ccggtaggca tgaaacgcta gcggagaacg
     2581 ctctccgctc tcgtccgcat accagatcat catgatcctt gctgcatgcg gataggggag
     2641 cggctgaaat agaattgggt tgagtgcgct gaaaatcgcc gtagcaccgc cgatgccgat
     2701 ggcgagaatc aggacagcag agcaggtgaa tcccgggctc ttgcggagct gccgcacgcc
     2761 atagcgcaga tcttgaatcc aggaattcat gcattggcat cctgcatcag cttgtcgagc
     2821 tcatcctcgg ctacgacctt tccgtcaaac aggtgcactt cgcgctgggc gtgtttcgca
     2881 aaccggggat cgtgggtcac catgcagatg gtcgcgccgt cgcgatgcag atcggcgagg
     2941 agttccatga ccgcctcccc gttgcgggaa tccagatttc ccgtaggctc gtccgccagc
     3001 agaatcgacg gcttcccgcc cagcgcgcgc gccacggcca cgcgctgctg ctggcctccg
     3061 gagagctggg aaggatagtg ccgcacccgg tgcgccatgc ctacgcgctc cagcgcttcc
     3121 attactcgct tcttgcgctc ggccgaaggc atgcgctggc ggtaggtcaa gggaagttcg
     3181 acgttctcat agaccgtgag gtcgccgatc aggttgaaac tctgaaaaat gaatccaatc
     3241 tcctggttgc ggatgcgcgc ccgctcggcg aagtcgagat tctcgactgc cttgctgttc
     3301 aacaaatatc ggccggaggt gggcgtgtcg agcagccc
//
LOCUS       DASPUL010000667         2260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_403678, whole genome shotgun sequence.
ACCESSION   DASPUL010000667 DASPUL010000000
VERSION     DASPUL010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403678"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VEJ00_15365"
     CDS             <1..261
                     /locus_tag="VEJ00_15365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02594.1"
                     /translation="TDFRDALNRVRNTAWAAQKYVASRISDEGPGGLTSFLASERVRC
                     AFQLCRSVQEDLQREDIRFQKGQLVQLQAAILGLNEQLQDRL"
     gene            complement(301..>2260)
                     /locus_tag="VEJ00_15370"
     CDS             complement(301..>2260)
                     /locus_tag="VEJ00_15370"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HXX02595.1"
                     /translation="AIVPQFFYLIYERIFQEIEKRGALTKKVFAAMVALNRTLRRIGI
                     NAGPVLFRKVHKTLGPKMRYLVTGGSRFDPEIARGFYDLGIDVLQAYGLTETTAAVFA
                     NAPNNNVIGSVGKAMKGVEARIVDAQPQDDGNPPVGEIALRGAVVMKGYWNRPDATAA
                     VLHDGWLLTGDLGYFDARENLFITGRKKEVIVLSNGKNVYPEEIEAHYLKSPFIKELA
                     VMGLEGKAGEGRDRLHGVIIPDFDVLRQRKIVNAKEVIRFDIEGLSQQIASTKRIGSY
                     EIWQEDLPRTTTRKIKRFEVEKRVKANQAKKLADDSDLPPEKPLTADEISWLDRPEVQ
                     RGLAVIRDTVHNAPPTLRPTHSLELDLGLDSMQRVELLSRLEDELGGNVEESQLAQIY
                     TVRELFDAVLQSAANGQAAPRATFAGWKAILAEEPTDPEVLALKDPHPAFDIFWYPLS
                     RIIQMTSRDLFHLRVSGLENLPRQGPFILSSNHQSYFDPVILTSVLPREFMEKTFAVG
                     TSEIFGEGFMRRLARWLRVIVVDPDANLVPAMRAGAYGLKHGRVLILYPEGERSIDGT
                     PRIFKKGAAILSIHMQVPIVPVAIEGFYEAWPRGKSFFQKLAPLEMVFGQPIQPLPEA
                     EASEAAYERLTADLKARIVAMWDKLRER"
BASE COUNT          436 a          657 c          691 g          476 t
ORIGIN      
        1 accgactttc gcgacgcgct caatcgtgtg cgcaacacgg cgtgggctgc tcagaagtat
       61 gtggcgtcac gtataagcga tgagggaccg ggcgggctga cgtctttcct ggcaagcgag
      121 cgagtgcgct gcgccttcca gctttgccgc tcggtgcagg aggacttgca gcgcgaggac
      181 attcggtttc agaagggcca gctggtacag cttcaggccg ccatccttgg gctgaacgag
      241 caactgcaag accggctgta gctttccagt gagattgaga tccgattcag aagttgccga
      301 tcaccgctct cgcagcttgt cccacatagc gacaatcctg gctttcaagt cggcagtgag
      361 ccgctcatag gcggcctcgg atgcttcagc ttcgggcagc ggctggatcg gttggccgaa
      421 gaccatttcg agaggcgcca gcttctggaa gaagcttttt ccccgcggcc aggcttcgta
      481 gaacccttcg attgcgaccg gaacgatcgg cacctgcatg tggatggaca ggatcgccgc
      541 gccttttttg aagatgcggg gagtgccgtc gatcgagcgt tcgccctcgg gatagagaat
      601 cagcacccgg ccatgcttca acccgtaagc tcctgcccgc atcgccggca caagattggc
      661 gtcggggtcg accacgatca cgcgcagcca gcgcgccagc cggcgcatga acccttcgcc
      721 aaagatttcg ctggttccga cggcgaaggt cttttccatg aattccctgg gcaggacgct
      781 ggtcaggatg accgggtcga aatagctctg gtgattggat gagaggatga acggaccttg
      841 ccggggtaag ttctccagcc cggagacgcg gaggtgaaac agatcgcgcg aagtcatctg
      901 gatgatgcgc gacagcggat accagaagat gtcgaaagcg ggatgggggt ccttgagcgc
      961 cagcacctca ggatcggtgg gctcttcggc gagaatcgcc ttccacccgg caaaagtggc
     1021 tcggggcgcg gcttgcccat tggcggcgct ctgcaggacg gcgtcgaaca actcgcgcac
     1081 ggtgtagatc tgggcaagct gggattcctc gacattgcct ccaagttcat cctcgagacg
     1141 gctgagcagt tcgacgcgct gcatggagtc aagccccaga tcaagctcca gactgtgtgt
     1201 ggggcgcaga gtcggtgggg cgttgtgcac ggtgtcgcga atgaccgcga gaccacgctg
     1261 cacttcagga cgatccagcc acgagatttc gtcggcagtc agaggctttt ctgggggcag
     1321 gtccgaatcg tccgcaagtt tcttcgcctg atttgccttg acccgcttct cgacttcgaa
     1381 gcgtttgatc ttgcgggtgg tggtgcgcgg cagatcctcc tgccagattt catagctgcc
     1441 aatgcgcttg gtcgaggcta tctgctgcga caggccttcg atatcgaaac ggatcacttc
     1501 cttggcgttg acgattttcc gttggcgcag cacgtcgaaa tcagggatga tcacgccgtg
     1561 caggcggtcg cgaccctcgc ccgccttgcc ctcaaggccc atcaccgcca gttccttgat
     1621 gaacggcgac ttcaggtagt gtgcctcgat ctcttccggg tagacattct tgccgttgct
     1681 caaaacgatc acttctttct tgcggccggt aatgaaaaga ttttcccgtg catcgaagta
     1741 gccgagatct ccagttagaa gccagccatc gtggagaacg gccgccgtcg cgtcgggccg
     1801 gttccagtag cccttcataa cgactgcccc tcgtagagcg atttcgccca caggagggtt
     1861 gccgtcatcc tgcggctgcg cgtcaacgat cctggcttcg acacctttca tcgccttccc
     1921 cacggatccg atgacgttgt tgttcggcgc attcgcgaaa actgccgctg tggtctcggt
     1981 caggccatag gcctgcagca cgtcgatgcc gagatcgtag aatccgcggg caatctcggg
     2041 gtcaaaacgc gatccaccag tgaccaggta gcgcattttc ggaccgagag tcttgtgaac
     2101 cttgcggaac aggaccggcc cggcgttgat gccgatcctg cgaagcgtgc ggttcaacgc
     2161 aaccattgcc gcgaagacct ttttggtaag cgccccgcgt ttctcgattt cctgaaaaat
     2221 ccgttcgtag atgagataga aaaactgcgg cacgatggcg
//
LOCUS       DASPUL010000668         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2053798, whole genome shotgun sequence.
ACCESSION   DASPUL010000668 DASPUL010000000
VERSION     DASPUL010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053798"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VEJ00_15375"
     CDS             complement(<1..203)
                     /locus_tag="VEJ00_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004083625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HXX02596.1"
                     /translation="MAAKNRYLFTSESVTEGHPDKIADQISDAILDACLTEDSSSRVA
                     CETLTATGLVVIAGEITTKAYVD"
     gene            complement(526..1671)
                     /locus_tag="VEJ00_15380"
     CDS             complement(526..1671)
                     /locus_tag="VEJ00_15380"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HXX02597.1"
                     /translation="MGQFGDKFKEAREKRGLSLDDVSNVTKIGSRMLQAIEEEHFDQL
                     PGGVFNKGFIRAYAKHLGLNDQEAVTEYLACLRQAQIDAQAAWNPQARPPAPEKRPLV
                     SEAKPSAKAIRDQPPPPREELPELQLPKAEHVRPPRPMYPEMGAGIPWRIVAVAGLVV
                     VLGIVWWSRRSHTAHPETTASAAPVPSSTSVQPATSPVSAPASAQKTAPEGGNAVTSA
                     PPSASKPQPSPVTPSAPEAPHSATRNALAEQTNPAGASALTKNAGETTEDTASTPVPQ
                     NPASQPPGKSAALMTLVIRASENSWISVTADGQPVSQETLIAPAHTSIRASHEIVVKA
                     GNAAGVSFLWNGKEIAAEGGEAEVKTFVFDSGGMRVVSTAQPATQNP"
     gene            complement(1791..2204)
                     /locus_tag="VEJ00_15385"
     CDS             complement(1791..2204)
                     /locus_tag="VEJ00_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02598.1"
                     /translation="MARKTLTNYALASAFFLLATAVLICQTTPAPDPRAVPVIDGGIG
                     PCYADLTITDASGAPVYAAKVNVHIAYGFMNVRKLDLELGTNTDGKARFTGLPSRIKH
                     GIYFRASEGDRTGEAFDDPANTCQAKFTITLRKPQ"
BASE COUNT          430 a          660 c          885 g          499 t
ORIGIN      
        1 aagtcgacgt aggctttcgt cgtgatttcg ccggcgatga cgaccaggcc ggttgcggtc
       61 aaagtctcgc aagcgacgcg gctggaggag tcctcggtga ggcaggcgtc gaggatggcg
      121 tcggaaatct ggtcggcgat cttgtcggga tggccttcgg ttacggattc agaagtgaat
      181 agatagcggt tctttgctgc caagttgtgt gtctccttaa ttccacgtgt gatcccggta
      241 tgggtcaggg acgtggtaag cagaatctct gggctccaga tctggcggga ccagcggtag
      301 agcggcgcgg ccacagcggc ggcgcgtcga aggtcaagtc ctgtaagagg ttatcagagg
      361 ggggtaaccg gcgcaacggg tttgtcgctg gggcgccgat aaaggagttc aagcgcggga
      421 tttcgcgcgt ggatgggcgg aatcggcggt tcggtggcaa cgggatgctt gcgcctgatg
      481 gtttccgggg agcggaagga gcaggagcca ttcccgcagc gggacctacg ggttctgggt
      541 agcgggctgc gctgtcgata ccaccctcat gcccccggaa tcgaagacga aggtcttcac
      601 ttcggcctcg ccgccctccg ccgcgatttc ttttccattc cagaggaagc tgactccggc
      661 ggcgtttccg gccttgacca cgatctcgtg cgaggcgcgg atggaggtgt gcgcgggagc
      721 gatcagagtt tcctgactca caggctggcc atcggcggtg acggagatcc aggagttctc
      781 cgatgctcga atgaccaggg tcatgagagc tgccgatttg cccggcggct gcgaggcggg
      841 attctggggc accggggtcg aggcggtgtc ctccgtggtt tctcccgcat tttttgttag
      901 agcggacgcg ccggccgggt tggtctgctc cgcgagagcg ttgcgggtgg ctgagtgggg
      961 cgcctcgggc gcggagggcg tcaccgggga aggctgaggt ttcgaagctg aaggtggagc
     1021 cgacgtcaca gcatttccgc cttccggggc ggttttctgc gcggaggcgg gggcactcac
     1081 gggcgaagtc gccggctgaa ctgacgtcga ggaaggaacc ggggctgcgc tggccgtagt
     1141 ttccgggtgg gcggtgtgcg agcggcggct ccaccagacg attcccagga caacgacgag
     1201 gccggcgacg gcgacgatcc gccatgggat tccggcgccc atttccggat acatggggcg
     1261 cggcgggcgg acgtgctcgg ccttgggcaa ctgcaactca gggagttcct cgcgcggagg
     1321 cggaggttga tcgcgtatgg ccttggcaga gggcttggct tcggaaacca acgggcgctt
     1381 ttccggcgcg ggtggacgag cctgcgggtt ccaggcagcc tgggcatcga tctgcgcctg
     1441 ccgcaggcag gccagatatt ccgttactgc ttcctggtcg ttcagcccga ggtgcttggc
     1501 ataggcgcgg atgaagccct tgttgaagac ccctccggga agctggtcga agtgctcttc
     1561 ttcaatggcc tgtagcatgc gtgagccgat ttttgtgaca ttggaaacgt catcgagcga
     1621 gagcccgcgc ttctctcgcg cttccttgaa tttgtcgcca aactggccca ccgagacccg
     1681 ccccgggcaa cgccgattcc gccctgatgg tgacgggatc atcatagggc aagcggaatg
     1741 agtgcgccac agtcattcgg ccaatggaaa gtaaccgccg aggagccgcg ctattgaggt
     1801 ttccgcaacg taatcgtgaa cttggcctgg caggtattgg cgggatcgtc gaacgcctcg
     1861 ccggtgcggt cgccctcgga ggcgcggaag tagatgccgt gcttgatgcg gctgggcagg
     1921 ccggtgaagc gggctttgcc gtcggtgttg gtgccgagtt cgaggtcgag cttgcggacg
     1981 ttcatgaatc cgtaggcgat gtggacgttg actttggccg cgtagaccgg ggcaccagat
     2041 gcgtcggtga tcgtcagatc ggcgtagcag gggccgatac cgccgtcgat tacggggacg
     2101 gccctggggt cgggggcggg agtagtctgg cagatcagaa cagctgtcgc gagcaggaaa
     2161 aaggccgagg cgagggcgta gttcgtaagt gttttcctgg ccatagttca taagattact
     2221 ccaaccgtaa aaagatggct tcgcgagcgg aaagccgatg atcccgttgg ctttaggggt
     2281 tccggttacc gacgttcatt gcccctgcac gaaggtgatg aatataattt tttgaactgc
     2341 caccccgtcc aacgaggaat attgggaact ttctgcatgc ctgaggccgg cactcaattg
     2401 acgcgccaga gccaggaaca gcggatcttt cacgacgtgg ccaaggcgct gacgtcgtcg
     2461 ctggatctcg actc
//
LOCUS       DASPUL010000669         2023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1357664, whole genome shotgun sequence.
ACCESSION   DASPUL010000669 DASPUL010000000
VERSION     DASPUL010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357664"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1708
                     /gene="amrB"
                     /locus_tag="VEJ00_15390"
     CDS             116..1708
                     /gene="amrB"
                     /locus_tag="VEJ00_15390"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HXX02599.1"
                     /translation="MGREKRSAKSVAVFFAVTFAFVALECSSAASPQNVRQPGVAGGF
                     YPADPQALSAMIDDMLAHASPPVIKDPILAVVAPHAGYQYSGPVAAYTYAALKGRKFS
                     RVVVIAPSHFEAFDFTSVYDGDAYATPLGTVSVDKTFAAQLAKMSSTIQLSSRGHLPT
                     KEGAEHALEVQLPWLQRALGDFVLVPVVMGDQSFESSRALGVALAKLIQGTDTLIVAS
                     SDLSHYHPYDDAVKIDHKTLSALQEWDYLSMSQNFETRVWEACGGAPIVAAMIAAERM
                     GANQALVLKYANSGDTTGDRSRVVGYSADVFVKAPSAAAVEQPFSLSGREKSELLALA
                     RKSVEYVVREKKVYEPGPSSSESLNQERGAFVTLKKSGELRGCIGYTSAMKPLYLAVR
                     DTATLAALRDPRFQPVSASEAPQLEYEISVLSPMRRVLDIRQIKVGQHGLLMRNGDHE
                     GLLLPQVPVEERWDRQQFLEETCAKAGMRSGCWKAEDTDIFMFTAVVFGEHGLSMLTP
                     ETVSPPALPARPGVSPPGSPPR"
     gene            complement(1632..>2023)
                     /locus_tag="VEJ00_15395"
     CDS             complement(1632..>2023)
                     /locus_tag="VEJ00_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02600.1"
                     /translation="ATLTQSLLALSAPALLGVLTVLSHSSSASGTLAVARIAFPVSAL
                     LCGMLGGFQFPIATKIYLGGASAQAGLGVLYVVDLLGGCAGALLLSTYFIPVFGFWRT
                     AWLSAAVNLAATLLAARVTLGEKLSQA"
BASE COUNT          410 a          584 c          628 g          401 t
ORIGIN      
        1 catgtactgg tcgagcgcgt tggtcttacg accggccaga tgctcatccg caagggcact
       61 tgccctttct gccaccaggc gatcccggga gtgtggcatg tgtgagggag gttgtatggg
      121 aagagagaag cgctctgcta aatccgtggc ggttttcttc gcggtcactt tcgcgttcgt
      181 agcactggaa tgctcgtctg ccgcttctcc acaaaacgtg cgccagcccg gcgtcgccgg
      241 tggcttctat ccggccgatc cgcaggcgct ctccgcgatg atcgacgata tgctcgcgca
      301 cgcctctccg ccagtgatca aagatccgat tctggcggta gttgccccgc atgctggcta
      361 ccagtactcc gggcccgttg ctgcctacac ctatgcagct ttgaaagggc gtaagttctc
      421 gcgcgtcgtc gtgatcgcgc cgtcgcattt cgaagctttc gatttcacgt cggtctacga
      481 tggcgacgcc tatgcgacgc cgcttggcac cgtttcggta gacaagactt tcgccgcgca
      541 gttggcgaaa atgagctcga ccatccagct ttcaagtcgc ggacatcttc ccaccaagga
      601 aggggcggaa cacgcccttg aggtgcaact gccctggttg cagcgtgcgc tcggagattt
      661 cgtgctcgtt ccggtggtca tgggagacca gagcttcgaa agcagccgcg ccttgggcgt
      721 tgcgctggcg aagctgatcc agggcacgga tactctgatc gtagccagtt ccgatttgtc
      781 gcattatcac ccctacgacg acgcggtgaa gattgatcat aaaaccctca gcgcgctgca
      841 ggagtgggac tacctgagca tgtcgcagaa tttcgagacg cgtgtctggg aagcctgtgg
      901 cggcgcgccg atagttgcgg ccatgatcgc ggcagagcgt atgggggcga accaggcctt
      961 ggtgctgaag tacgcaaatt ccggcgacac gaccggggat cgctcgcgcg tggtcggata
     1021 cagcgcggac gtattcgtga aagctccaag cgcggccgcc gtggagcagc cattctcgct
     1081 cagtggccgc gagaaaagtg aactgctcgc tctggcgcgc aagtccgtgg aatacgtggt
     1141 gcgcgagaag aaagtgtatg agccggggcc aagttcgagc gaatcgctga atcaggaacg
     1201 tggggcgttc gtcactctga aaaaatcggg cgagttgcga gggtgcatcg gctatacctc
     1261 ggcaatgaaa cctctttatc tggccgttcg cgacacagct acgctggctg cgctacgtga
     1321 tccccgtttc cagccggtat ctgcctcgga ggcaccacag ctggaatacg aaatctctgt
     1381 actctcgccc atgcggcgcg tgctcgatat tcgacagatc aaggttgggc aacacggtct
     1441 gctcatgagg aacggcgatc atgaaggcct tctgctcccg caggtcccgg tcgaagaacg
     1501 gtgggatcgg cagcagttcc ttgaagaaac ctgtgccaag gcgggcatgc gatccggttg
     1561 ctggaaggcc gaagatactg acatcttcat gttcacagcc gtggtattcg gcgagcatgg
     1621 gttatcgatg cttacgcctg agacagtttc tcccccagcg ttacccgcgc ggccaggagt
     1681 gtcgccgcca ggttcaccgc cgcgctgagc catgccgttc tccagaaacc gaacacggga
     1741 atgaaataag tgctgaggag cagcgcccca gcgcacccgc ctagcaagtc aaccacgtac
     1801 aacacaccga ggccggcctg tgcgctggct ccgccgaggt agattttcgt tgcgattggg
     1861 aattggaagc cgcccaacat gccacatagc agcgccgata ccgggaaagc aatccgagca
     1921 acggccagcg tgccggacgc actcgaactg tgcgatagaa cggtcaaaac accgagcagc
     1981 gcaggtgcag aaagggcgag cagggattga gtgagggttg cga
//
LOCUS       DASPUL010000670         3436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_2053910, whole genome shotgun sequence.
ACCESSION   DASPUL010000670 DASPUL010000000
VERSION     DASPUL010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053910"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VEJ00_15400"
     CDS             <1..354
                     /locus_tag="VEJ00_15400"
                     /inference="COORDINATES: protein motif:HMM:NF039204.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HXX02601.1"
                     /translation="QVNSAVRTVKVQRKLRRHNRNAAPSDGDTASSHLAGVTVDLQRR
                     GMTKEQIRWMEHYLFYMKALGLVEPEEERRHWCFHIMVSGHYSEWRETQDIVPMEHPD
                     QPAPETQVAMGSGSN"
     gene            complement(387..770)
                     /locus_tag="VEJ00_15405"
     CDS             complement(387..770)
                     /locus_tag="VEJ00_15405"
                     /inference="COORDINATES: protein motif:HMM:NF025903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HXX02602.1"
                     /translation="MPLVRISLRGGKSEQYRRALADGVHQAMVEAIDAPVQDRFQIVT
                     EHSANDLIYDPNFFGIERGDDIVIVQITLSAGRKPPQKRKLFQRMAEILSKNPGLRPQ
                     DLMINLVEVVWENWSFGNGEAQYTL"
     gene            818..1366
                     /locus_tag="VEJ00_15410"
     CDS             818..1366
                     /locus_tag="VEJ00_15410"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXX02603.1"
                     /translation="MIVLMAGSPGTGKTTLARALARHINGRVISKDEFRHAVFSPDEV
                     EYSTWQDDFCLHLMLQTAEYLLARDANLIVFLDGRTFSRRYQIENVIAAATRMHQAWR
                     ILECICSDETIRRRLAADAEDRTHPAGNRDPQLYLEVKARFEAITRPKLVIDTEQGLE
                     LSIRRALQGLNLADTGILEGNP"
     gene            complement(1404..2066)
                     /locus_tag="VEJ00_15415"
     CDS             complement(1404..2066)
                     /locus_tag="VEJ00_15415"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXX02604.1"
                     /translation="MGGLIPLRDASRRPVRVPLCTGLIILANALVFLLELMRGDAFVM
                     QWSAVPQQIVSGHHWITILTAMFMHGSWSHIIGNMVFLWAFGPEIEDAMGRGRYLAFY
                     LLGGLVAMLAQVAASPHSTVPNLGASGAIAAVMGAFIVTYPRDRIKSLLLIFIFVKVT
                     FIPAALLIGVWFLTQLIHAGQVAQVQTGGVAYLAHVGGFIFGAITARWFEVSQVALQE
                     EN"
     gene            complement(2143..2718)
                     /locus_tag="VEJ00_15420"
     CDS             complement(2143..2718)
                     /locus_tag="VEJ00_15420"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HXX02605.1"
                     /translation="MVQSPSDVVRQVSTLSILWGVLLIGLGMLAVASPLVAALAVNVL
                     VAWLIMLAGVVHLIVAFHTREAGSMIWRVLVGVAYVCFGAYLVARPAVGVASLTLVLA
                     SLFLVEGILNIVLFFRVGPLLRSNWILLDGIVTLLLGLMIYMQWPSSSAWAIGTLVGV
                     SMIFSGVTRVMISLAVRKAATAAHPPTSLAA"
     gene            2893..>3436
                     /locus_tag="VEJ00_15425"
     CDS             2893..>3436
                     /locus_tag="VEJ00_15425"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HXX02606.1"
                     /translation="MTTRLFFSLVLLCSFATAQSAQESSPGPGIIVSAKARAIHEAAL
                     VIDTHADTPQRFLDGGFDIGSTDSNDPGHISLDKIRRGNLGAEFFSIWVEPETNEGHF
                     ARHTFDLIDSVYEQATRHPDRMMMAFSVADIERAHAEHKLAALMGIEGGHSIENDIHL
                     LRDYYRLGVRYMTLSWSNTNE"
BASE COUNT          807 a         1057 c          916 g          656 t
ORIGIN      
        1 caggtcaact cggccgtgcg cacggtcaag gttcagagaa aactgcgccg ccacaatcgc
       61 aatgctgcgc cctccgacgg cgataccgct tcttcgcacc tcgccggcgt gacggtcgat
      121 ctacaacgcc gtggcatgac caaggaacag attcgctgga tggagcacta cctgttctac
      181 atgaaggctc tcggcctggt tgagcccgaa gaggaacggc gccactggtg cttccacatc
      241 atggtcagcg gccactactc cgaatggcgc gagacgcagg acatcgttcc catggaacac
      301 cccgatcagc cagcgccaga aactcaggtc gccatgggca gcgggtcgaa ctaacggatt
      361 ccctttctcc ccacatcttc tgacttctac agcgtgtatt gcgcttctcc gtttccgaac
      421 gaccagttct cccagacaac ttccaccaaa ttgatcatga gatcctgcgg acgcagtccg
      481 ggattcttgc ttaggatctc ggccatgcgc tgaaatagct tgcgcttttg gggaggcttc
      541 cgtccggcag acagagtgat ctgtactatc acaatgtcat ctccgcgctc gattccaaaa
      601 aagttcgggt cgtagatcag atcgttggcg gagtgctccg tgacgatttg aaagcgatcc
      661 tgcaccggcg cgtcgatcgc ttcgaccatg gcttgatgaa cgccgtcagc caaggcgcgg
      721 cggtattgtt ccgacttccc cccacgtaaa gaaatgcgca ccaaaggcat taaggtctcc
      781 tcagaagcag attgaagttt ctgctctaat gatacccatg atcgtcctga tggctggctc
      841 gccgggcacg ggcaagacta ctcttgctcg tgcgctagcg agacacatca acggccgcgt
      901 catcagtaaa gacgagttcc ggcacgcagt cttttctcct gacgaggtcg aatattccac
      961 ctggcaggac gatttctgtt tgcacctcat gctgcagact gcggagtatc tcctagcgcg
     1021 ggacgcgaat ctgattgtct tcttagatgg ccgaacattt tcgcgccgct accagatcga
     1081 gaacgtgatc gcagccgcca cgcgcatgca ccaggcttgg cgcattctgg agtgcatttg
     1141 ttctgacgag accatccggc gcagattagc tgctgatgct gaggaccgca ctcatccagc
     1201 gggcaaccgc gatccccaac tctatctgga agtgaaggcc cggtttgaag ccattactcg
     1261 gcccaaactt gtaatcgaca cggagcaggg cctggaactc tccattcggc gcgcgctgca
     1321 aggcttgaac ctcgcagaca ccgggatact cgaaggaaat ccctgatgcc ccttgttagg
     1381 ttgcgacccc cagcaccgca cctctagttc tcttcctgca aagcgacctg tgagacttcg
     1441 aaccagcgag ctgtgatagc gccgaagata aagccaccaa cgtgcgccaa gtacgccaca
     1501 ccgccggttt gcacttgcgc cacttgcccc gcatgaatga gctgagtgag aaaccatact
     1561 ccaatcagaa gcgcagccgg gatgaaggtc actttcacaa agatgaagat cagcaggagc
     1621 gacttgatgc ggtcgcgcgg ataagtaacg ataaatgcgc ccatcaccgc ggcgatagct
     1681 ccgcttgccc cgaggttcgg aactgtggag tgcggactgg ctgcgacctg agctagcatc
     1741 gccaccaaac cgccaagcag gtaaaaagcc agatatcgtc cgcggcccat cgcatcctcg
     1801 atttccggcc cgaatgccca gagaaatacc atgttgccga tgatgtgcga ccagcttccg
     1861 tgcatgaaca tggcggtcag aatcgtgatc cagtggtgac cagatacgat ctgctggggc
     1921 accgccgacc actgcataac aaatgcgtcg ccccgcatca gctcaagcag aaaaacgagg
     1981 gcgttcgcga gaatgatgag gccggtacaa aggggaacgc gcacaggtcg gcgcgacgcg
     2041 tcacgtaaag gaattaggcc tcccatgtca cgctcctcgg ttcacatgga atagacaagg
     2101 ctgtccttct gccagaaacg agcccgttaa ccggcgggca ctttaggctg ccaacgatgt
     2161 cggcgggtgc gcggctgttg cggccttgcg cactgccaaa gagatcatca cgcgcgtgac
     2221 gccgctgaaa atcatgctca cgcccaccaa tgtcccgatg gcccatgcgg aactcgacgg
     2281 ccactgcatg tagatcatca agcccagtag cagcgtaacg attccgtcga gcaatatcca
     2341 attcgaccgc aacaagggcc ccaccctgaa aaacaagacg atgttcagga tgccctcaac
     2401 cagaaacaac gacgccagca ccagagtgag cgaagctaca cccacggccg gacgcgccac
     2461 caggtacgcg ccaaaacaca cataggcaac cccaaccaga actcgccaga tcatgcttcc
     2521 cgcttcccga gtgtggaagg caacaatgag atggaccaca cccgccaaca tgatcagcca
     2581 ggcaactaat acgttgaccg cgagcgcagc gaccaaaggt gatgctacgg ctaacatgcc
     2641 taagccaatc agcagcactc cccagaggat tgacagtgtc gaaacctgtc gcacgacgtc
     2701 ggagggactt tgcaccatag cgacctcctc aactgattcg gggagtctat cccaaaggaa
     2761 tcgcgcaggc aatcgaaacg caggacgcgt ctgagagagc agcgtactcg gcaaggcctg
     2821 ggctcctttt gacggccaag gttctgccta tacaatcaag gacctgccca caatcttctc
     2881 gggcgcacca ctatgacaac caggcttttc ttttcccttg tactgctatg ttcgttcgct
     2941 acggcccagt cggcgcaaga gtcttcgccc ggaccgggta tcatcgtcag cgcaaaggcc
     3001 cgtgccattc acgaggcggc gttggtgatc gacacgcacg ccgacacgcc gcagcgattc
     3061 ctggatggag gtttcgacat cggctcgacc gactcgaacg atcccggcca catcagcctc
     3121 gacaagatcc gccgcggaaa cctgggcgcg gaatttttct caatttgggt ggaaccggag
     3181 actaatgaag gccacttcgc ccgtcacacg ttcgatctga ttgactccgt ctacgagcaa
     3241 gcaacacggc atcccgatcg catgatgatg gcattctccg tcgccgatat cgagcgcgct
     3301 cacgccgagc ataaactcgc ggcgctgatg ggtatcgaag gcggtcactc catcgagaac
     3361 gacattcacc tgttgcggga ctactaccgc ttgggtgtcc gctatatgac gctgtcctgg
     3421 tcgaacacga acgagt
//
LOCUS       DASPUL010000671         3968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1632981, whole genome shotgun sequence.
ACCESSION   DASPUL010000671 DASPUL010000000
VERSION     DASPUL010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632981"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..112
                     /locus_tag="VEJ00_15430"
     CDS             5..112
                     /locus_tag="VEJ00_15430"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXX02607.1"
                     /translation="MGRNPQTGEPIQIKAKTVVKFRVAKAAKDAIAPKK"
     gene            180..1127
                     /locus_tag="VEJ00_15435"
     CDS             180..1127
                     /locus_tag="VEJ00_15435"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXX02608.1"
                     /translation="MLNWIVIGIGDITTRRVIPAIQAEPRSCLYGIVTRDPAKSASYN
                     TRTWPTLDDALNNPAVRAEVQVIYVATPVFLHAPQTIQALRAGKHVLCEKPMAMNEPE
                     ARGMLQAAEENGKTLGVAYYRRCYPKVQRAKQLIEAGAIGKPVLAELACHGWFDGLGE
                     RSWLVDPSKAGGGPLFDIASHRIDVLNFLFGQPLRGTSQLSNVIHRYAVEDNATVMIE
                     YAGGVRGIVDVRWHSHVDRDECRIRGTDSEMELSPLNGPEIVYPGGRENLPPHANLHY
                     PIVENFVEAVLDQRPLLSSGATAYWTDWVAEQARRQEVQ"
     gene            complement(1356..2648)
                     /locus_tag="VEJ00_15440"
     CDS             complement(1356..2648)
                     /locus_tag="VEJ00_15440"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXX02609.1"
                     /translation="MRKLHSIAVVAILLGCWAGAMAQESHRVVVRAGRVLDVKTGKLL
                     ADQALVIEDGKIVSMGAAAESKSAADAVRIDLPNATLLPGLIDAHTHLTMDPRFGYEQ
                     LAISAPREALTGAKNARLTLLAGFTTVRNVGASDYTDVALRDAINAGDVPGPRMLVSG
                     PALSITGGHCDNNLLPFEYHATSDGVADGIAAVQHKVRENIKYRVDLIKVCATGGVLS
                     MGDNPQHSQYTLEEMKAIVADAHRLGRKVAAHAHGAEGIRWAAEAGVDSIEHGSYIDD
                     AAIAVMKEHGTYLVPTLWLGDWMFANAEAIHMPAPLLAKAHEVIPAARKNVAHAFASG
                     VKVAFGTDAAVYPHGLNAHEFAVMVKLGLTPLQAIQAATINAADLLGWSGKVGTLEPG
                     AWADIVAVEGDPLKDVTTLERVKFVMKGGEVVKNEYGK"
     gene            complement(2686..3162)
                     /locus_tag="VEJ00_15445"
     CDS             complement(2686..3162)
                     /locus_tag="VEJ00_15445"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HXX02610.1"
                     /translation="MNTSFSSRTIAGVRIATSLFFLFFGEYKVAGPKFAHGGFQAYLR
                     DYIASSAIGFYRPFLSGIILPHAVFFGYFVGVLELLVGVSLLLGLWVRPACVLGIFFL
                     LNLTLAAWWDPGHGVPVWRYFGARLDTLPLMLLLIIFFAANAGQVWGLDGRRRQGP"
     gene            complement(3221..3658)
                     /locus_tag="VEJ00_15450"
     CDS             complement(3221..3658)
                     /locus_tag="VEJ00_15450"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXX02611.1"
                     /translation="MSVLELCDREIAAVGLDATAAEAIHKMLDRRVGAVAVVDSEYRV
                     AGIFTERDVLRKMALSRVDPETTPVRDLMTTPVEMATRDTGPGEALTTMLERHFRHLP
                     VADNSGKLLGILSIRNLLEWRVDDLSRELESLEQYVSNDGPGG"
BASE COUNT          780 a         1319 c         1138 g          731 t
ORIGIN      
        1 ccgcatcgga cgcaacccgc agaccggcga acccatccag atcaaggcca agaccgtggt
       61 gaagttccgc gtcgccaagg ccgcgaaaga cgcgatcgct cccaagaagt agttcccgtc
      121 agaaatgaac aaaaggccgg cccaggccgg cctttgcctt tatcggttct gtggactcca
      181 tgctcaactg gatcgtcatc ggtatcggcg acatcaccac ccgccgcgtg atccccgcga
      241 ttcaggcgga gccgcgcagc tgcctttacg gcattgtcac ccgcgatccg gcgaaatcgg
      301 cttcgtacaa cacccgaacc tggccaacac tggacgacgc actcaataac cccgcagtcc
      361 gtgcggaagt tcaagtcatc tacgtagcca cgcccgtctt cctgcatgcg ccgcagacga
      421 ttcaggcgct ccgcgccggc aagcacgtgc tttgcgaaaa gccgatggcg atgaacgagc
      481 cagaggcgcg cggcatgctg caggcagcag aggagaacgg caagacgctc ggcgtggcct
      541 actatcgccg ctgctatccc aaggtgcagc gcgcgaagca gctgatcgaa gctggagcga
      601 tcggcaagcc cgtgctggcc gagctcgcct gccacggctg gtttgatgga ctaggggaac
      661 gaagctggct ggtcgatccc tccaaagccg gaggcgggcc gctattcgat atcgcttcgc
      721 accgcatcga tgtgctgaac ttcctgttcg gccagccact ccgcggcacc agccagcttt
      781 cgaacgtgat tcatcgctat gccgtcgaag acaatgcgac ggtgatgatc gaatacgctg
      841 gcggagttcg cgggatcgta gacgtgcgct ggcattccca cgtggaccgc gacgaatgcc
      901 gcatccgcgg caccgacagc gaaatggagc tgtctccgct gaacgggccg gagatcgttt
      961 atcccggcgg ccgcgaaaat cttcccccac acgccaatct gcactatccg atcgtcgaga
     1021 acttcgtgga ggctgtgctg gaccagcgcc cgctgttatc gagcggagcc accgcttact
     1081 ggacggattg ggtcgccgag caggcccgac gccaggaagt gcaatgacct tcagacagcc
     1141 gccttcagcg ccgaaccgcc aaaaaccctt gggtggcgca gcgcttcagc gctgcggtac
     1201 tactgtgatc attctgtcgg ggcttctgct ccggaggtac ctcggcggct gaagccgccc
     1261 ttcaaaggct gcgattgaac gcagcgcttg aagcgctgcg ctaccccaaa ccagactcca
     1321 agcccagaat ttttgtggga tgcgaggggc gaatctcact tcccatattc gttctttacc
     1381 acttcgccgc ccttcatcac gaactttacc cgttcgagcg tggtgacgtc ctttaacgga
     1441 tcgccttcga cagcgacgat atcggcccac gcaccgggtt ccagcgttcc gaccttgccc
     1501 gaccaaccga gcaagtccgc ggcgttgatg gttgcagctt gaatcgcctg cagcggcgtc
     1561 aggccaagct tcaccattac tgcgaactca tgtgcgttca gcccgtgggg atacaccgcg
     1621 gcgtctgtgc cgaatgcgac cttgactccg ctggcgaatg cgtgcgccac gttctttcgc
     1681 gccgccggaa tcacctcgtg cgccttggcc agcagcggcg ctggcatgtg tatggcctcg
     1741 gcattcgcaa acatccagtc gccgagccag agcgtcggca ccagatatgt gccgtgctct
     1801 ttcatcaccg caatcgccgc atcgtcgatg taagacccgt gctcgatgga atcgacgccg
     1861 gcctccgccg cccaacggat cccctcggcg ccgtgcgcat gcgccgccac cttgcgcccc
     1921 agccggtgcg cgtccgccac gatggccttc atctcttcga gcgtgtactg cgagtgctgg
     1981 ggattatcgc ccatcgacag caccccgccg gttgcgcaca ccttgatcaa gtccacgcgg
     2041 tacttgatgt tctctcggac cttgtgctgt acggcggcaa tgccgtcggc cacgccgtcg
     2101 ctggtggcgt gatattcgaa gggcagcagg ttgttgtcgc agtgtccgcc ggtaatgctc
     2161 agtgccggcc cgctcaccag catgcgcggc cccggaacgt cgccggcatt gatggcatcg
     2221 cgcagggcaa catcggtgta atcgctggcc cccacgttgc gcaccgtggt aaatccagcc
     2281 agcagggtga gccgcgcatt cttggcgccg gtcagtgctt cgcgcggagc cgaaatcgcc
     2341 agctgttcgt agccaaatct cggatccatg gtcaggtgcg tatgcgcgtc gatcagaccg
     2401 ggcagcagcg tcgcgttggg caggtcgatg cgcacggcat cagcggcgct tttgctctcg
     2461 gcggctgcac ccatactcac gatttttcca tcttcgatca caagcgcctg gtcggccagc
     2521 agcttgcccg tctttacgtc gagcacgcgc ccggcgcgca ccacaacccg atgcgattcc
     2581 tgagccattg ccccggccca acaacccagc agaatggcga caaccgcaat actgtggagc
     2641 ttcctcatga tgctgttcct ttcttgatcg tgacgcttgt tgaggctacg gtccttgccg
     2701 cctacgccca tccagtcccc acacctgtcc ggcattggcc gcgaagaaaa taatcagcag
     2761 caacatcagg ggcaaagtat cgagccgggc gccaaaatat cgccacactg gcactccgtg
     2821 gcccggatcc caccacgcag ccagggtgag attcagcagg aagaaaatac caagaacgca
     2881 ggcgggcctc acccagagcc caagcagcaa cgagacgccg accagcagtt caagaacacc
     2941 gacgaagtac ccgaaaaaga cggcgtgcgg caggattatc ccggatagga acggccggta
     3001 aaaaccgatg gcgctgcttg cgatgtagtc gcgcagataa gcctgaaatc cgccatgtgc
     3061 gaatttcggt ccggccactt tgtattcacc gaagaaaaga aaaaacagcg aagtggcgat
     3121 gcgcacacca gcgatggtcc tggacgaaaa ggaagtattc atggatcagt agtcagtcga
     3181 cggtcgattt taactgaccc ctgatcccta gcccctggcg ctaccctccc ggcccgtcgt
     3241 tcgaaacgta ctgctcgagc gactccagct cccggctcag gtcgtcgact cgccactcga
     3301 gcagattgcg gatggagaga atcccgagca gcttgccgct attgtcggcc accggcaaat
     3361 gccggaagtg tcgctccagc atggtggtga gagcctcgcc cggaccggta tcccgcgtcg
     3421 ccatctccac cggtgttgtc atcaggtccc gcaccggcgt ggtttcaggg tcgacgcggc
     3481 tgagcgccat cttccgcagc acatcgcgtt cggtaaagat ccccgcgacg cggtactcac
     3541 tgtcgaccac cgccactgcg cctacgcgac ggtcgagcat cttgtgaatg gcttcggcgg
     3601 cggttgcatc caggcccacc gccgcaatct cgcggtcaca cagttccagc acgctcataa
     3661 ccacctcctc gggctactct tgcccgaagc gccttcgaaa caggattgac gcattatgcg
     3721 cgtggccttt cccttgtcaa ggaaatttac gaagaattcc cgccgacgaa cccgccccga
     3781 ccgtttctcg tcccgatcga ggaatacctc ctcacgcggc aggcgtcatc ccgaaggccc
     3841 gcgctttcac cagcgggccg agggatctcg cgtggatcgg cttcactggc actggagatc
     3901 cctcgctgcg ctcgcccccg gctaaagccg gaggtctacc cgaacggctg cgctcgggat
     3961 gatgccgc
//
LOCUS       DASPUL010000672         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1907126, whole genome shotgun sequence.
ACCESSION   DASPUL010000672 DASPUL010000000
VERSION     DASPUL010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1907126"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1341
                     /locus_tag="VEJ00_15455"
     CDS             <1..1341
                     /locus_tag="VEJ00_15455"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXX02612.1"
                     /translation="LGGGMDLPLTKSIAFRLFEADYVWSRHNYADDAAQQFSSLRRPN
                     MEGVRLRTGLVFSWGGAPAVTPAAACTVQPAEVMVGEPITANVTASNFNPKHTLTYSW
                     SGTGGQVTGKDTTASIDSTNAAPGSYTVTAKVTDPKEKKNNEASCSANYTVKPLPPKN
                     PPTMTLSADPASVVMGSNGAITASCTSPDGVPVSVANWTSSAGSVSGSGNSATLNTTG
                     VPAGPVTVTATCTDSRGLTAQASTQVAVQNPPPPPVDKALEARLSLHSIYFPTAMPPV
                     KDPNAGLVNSQQATLTALAADFLTYLKSKPDAHLILEGHADPRGSVQYNQALSERRVN
                     RVKSFLVEHGVPEGDIDTKALGDQHNLTADEVKASVEQNTNLTKEERARTLRNMRTII
                     LASNRRVDVTLSTTGEVSTRQFPFNAEDALTLIGGRTPVKSAAPAKKKAAPKKK"
     gene            1696..2355
                     /locus_tag="VEJ00_15460"
     CDS             1696..2355
                     /locus_tag="VEJ00_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02613.1"
                     /translation="MSLIVGLDGFRRCAGWIVLPILLFAAGCTSPGGNGSGDGVAHAG
                     QPQAPFQDRDNGTSPNGGAQSAIDGPKAEAALPFRDATSLPAGTMITVRLQNPLTTQD
                     PDAPGTFDAVVDSSVVIEGNTMVPRGAAVTGRVESALASKVKRNQGYVRLTLASLSIA
                     GQSLPVQTSSLFARANAGDSADSEGDASSSAVHLEKGRRLTFRLTEPVLLASQPSIPG
                     R"
     gene            2575..>3494
                     /locus_tag="VEJ00_15465"
     CDS             2575..>3494
                     /locus_tag="VEJ00_15465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXX02614.1"
                     /translation="MSAAAHFEWSPDPGTELRILFDNAPFGVAQCERQGYITALNPAL
                     ARILGLHARLAEPMRFADLMASRDHAESARVLRELFDGKRDSFQIDSDLKARDGRALR
                     WTAWRVPGKNGSPDYALALAAETAESREAELRLRRAERLEALGRVAGGVAHDFNNLLT
                     GVLLCCDLLMAGLEPGHPSRKYAEEVRSAGTQAAGLVRQLLAMARPTDFAPRVLCINE
                     IAEGMRDLLARLIGENIDLTLHLDPCLGLIKMDPAQVQQIVLNLVLNARDAMPGGGRI
                     SVETSNCRVEALTGCGVCTSRVAAIPCSLLV"
BASE COUNT          677 a         1050 c         1095 g          672 t
ORIGIN      
        1 ctcggcggcg gaatggatct gcccctgacc aagtccattg cgttccgcct atttgaggcg
       61 gactatgtct ggtctcgtca taactatgcc gatgatgccg cgcagcaatt ctcttcgctg
      121 cggcggccca atatggaggg cgtgcggctt cgcaccggac tggttttcag ctggggcgga
      181 gcgcctgcgg taacaccggc cgctgcttgc actgtgcagc ccgcagaagt gatggtcggc
      241 gagcccatca ctgccaacgt tacagccagc aatttcaatc ctaagcacac attgacctac
      301 agctggagcg gcaccggcgg ccaggtcacc ggcaaggaca cgaccgcgag catcgacagt
      361 acgaatgcag ccccgggcag ctacacggtc acggcgaaag tgaccgaccc gaaagaaaaa
      421 aagaacaacg aagcaagttg ctcagcgaac tataccgtga agccgctgcc gccgaagaac
      481 cctccgacca tgacattgtc ggccgatccg gcgagcgtgg ttatgggcag caacggcgcg
      541 atcaccgcgt cctgcaccag ccccgatggc gttccagtct cggtggccaa ctggacctcg
      601 agcgcgggct cggtctccgg cagtgggaac tcggcgacgc tgaatactac gggcgttccg
      661 gcgggaccag tgacggtaac tgcgacctgt accgattctc gcggtctgac cgctcaggcc
      721 tcaacccagg ttgcggtgca aaatccgccg ccaccgccgg ttgacaaagc cctggaagcg
      781 cggctctcgc tgcacagcat ctacttcccg accgcgatgc cgccggtcaa ggatcccaat
      841 gccggcctgg tgaacagcca acaggcgacg ctgactgcgc tggcagccga cttcctgacg
      901 tacttgaagt ccaagccgga tgcacacctc attctggaag gccacgctga cccgcgcggc
      961 tcggtccagt acaaccaggc actctcggag cggcgtgtca accgcgtcaa gagcttcctg
     1021 gtggagcacg gcgttcctga gggcgacatc gacaccaagg ctctagggga tcaacacaac
     1081 ctgactgctg acgaggtcaa ggcttcggtc gagcagaaca cgaatctgac taaagaagag
     1141 cgggcgagaa cgctccgcaa catgcggacg atcattctgg ccagcaatcg ccgtgtggac
     1201 gtaaccctca gtacgaccgg ggaagtctcc actcgccaat tcccgttcaa cgctgaggac
     1261 gctctcacgt tgatcggtgg caggactccg gtcaagagcg ctgcgcctgc gaagaaaaag
     1321 gcggcaccga agaagaaata gcagttgcgt gggttagtgg tcgcggagag cgaacccgct
     1381 ctccgcgatt ttttttggtg ggaacgaatg gctaggcatg ttcgctctgg tcttcattgg
     1441 aggcgcttct ttcccacccg gagggggtgc cttggttggt ccatccggtc ccggggctat
     1501 tgccggtctc accttgccaa ttccatcact caaggctgtg gacgcggctt ttcaccaaaa
     1561 aagagggcac gtatgggtgc ggcaaaatga aaaatctaca tgggacacca cgaaagtgcc
     1621 atcgccaagt tgttgatttg atgcgactgt gtcctggtaa tccggttgcc aatgttcata
     1681 cgaaggcggg cgggaatgag tctgattgtg gggctcgacg ggttccggcg ctgtgcgggc
     1741 tggatcgtct tgccgatcct tttgtttgcg gcgggctgta ccagtcccgg cgggaatggt
     1801 agcggagacg gggtggctca cgctggccag ccgcaggccc ctttccagga tagggataac
     1861 gggacttccc cgaatggcgg ggctcagtca gcgattgatg gcccgaaggc tgaagcggcc
     1921 ctgccgtttc gcgatgccac cagcctgccg gccggcacga tgatcaccgt gcgccttcag
     1981 aacccgctga ctactcagga tcccgacgcc cccgggactt ttgacgcggt ggtggacagc
     2041 tccgtggtca tcgaaggcaa caccatggtt ccgcggggcg ccgccgttac cggacgggtt
     2101 gagtccgccc tcgcctctaa agtgaagcgc aatcaggggt atgtacgcct gacgctggcc
     2161 tcgctttcga tcgccggcca gagcctcccg gttcaaacct ccagcctttt cgctcgcgcg
     2221 aatgcaggcg acagtgccga ttctgagggc gatgcttcat cgtctgccgt gcatttggaa
     2281 aaggggcgac gccttacctt ccggctcacc gaaccagtac tgctcgcgag ccagccttca
     2341 attcctggtc gctagacgct ctttcctggg ctgccgccaa gttttccaca gaaagatttc
     2401 ccttcttagt gacgaacgca cttagcaggc acaggccttg ttctctatag tgggccggaa
     2461 aatgggagtc gcagttagcc gggggactcg acattcccat tctgggaaaa accaaagtct
     2521 gtggctgggg gattccatgt tcctttcggg aatcatcccg cgcagagtag agctatgagc
     2581 gctgctgccc atttcgagtg gtcgccggat ccgggaacgg aactgcggat cctgtttgac
     2641 aatgcgcctt tcggagtggc gcagtgcgag cgtcaaggct acatcacggc gctgaatccg
     2701 gcgctggcgc ggatcctcgg cctgcacgcg cgactcgcag agcccatgcg ttttgcagac
     2761 ctgatggctt cgcgggatca cgccgagagt gcacgggtac tgcgcgaatt gttcgacggg
     2821 aagcgcgaca gctttcagat cgattctgat ctcaaggcgc gcgatggccg ggcattgcgc
     2881 tggaccgcgt ggcgggtacc gggaaagaac ggatcacccg actatgctct ggcattggcc
     2941 gcagagaccg ctgagagccg cgaagccgag ctgcggctcc gccgggcgga gcgattggaa
     3001 gctctgggac gcgtggccgg tggcgtggct cacgacttca acaacctgct gaccggggtc
     3061 ctgctgtgtt gcgacttgct gatggcgggg ctggagcccg gacacccgtc acgcaagtat
     3121 gcggaagaag ttcgcagtgc gggcacgcag gctgccgggc tcgtgcgcca gttactcgcc
     3181 atggcccggc cgacggactt cgcaccccgg gtgctctgca tcaatgagat cgccgaaggc
     3241 atgcgtgatc tgctggcgcg gctgattggc gagaacattg acctgacgct gcatctcgat
     3301 ccttgcctcg gcctgatcaa gatggacccc gcgcaggtcc aacagattgt cctcaacctt
     3361 gtgctcaatg cccgggatgc aatgcctggc ggagggcgga tctcggtgga gaccagcaac
     3421 tgccgggtcg aagcgcttac tgggtgcggg gtttgtacca gtcgtgttgc agcaattccg
     3481 tgctctttac tcgt
//
LOCUS       DASPUL010000673         8634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1086806, whole genome shotgun sequence.
ACCESSION   DASPUL010000673 DASPUL010000000
VERSION     DASPUL010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086806"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VEJ00_15470"
     CDS             complement(<1..1391)
                     /locus_tag="VEJ00_15470"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HXX02615.1"
                     /translation="MNTGTTQTSAAPAVGQEKLAALFDRSPKALLWSVFREDRFFLVL
                     SVFIGVFSGLAVVCFRFAIDWCRIYLLGSGIVLTPARLVLAPTLAGLVIAALVMRVFP
                     LARGSGVNQTKAALYIYNGYIPLRTAVGKFITAALAIGSGHSLGPEDPSLQIGASLAS
                     ALGRRMNLSRDRMRLIAPVGAAAGLAAAFNAPISAVLFVIEEVIGRWTAGILGSVVLS
                     AVSSVVVMRWFLGSESLFRIPAVEMRRPSELLAYSVLGLVGGLASVMFSAGITTLRPR
                     CKALPRWTQYFQPALAGLLIGMIAVLGAPQVMGAGYEYIDEAMHGRFTWQFLAILAGL
                     KILATMLSFVSGTPGGMFAPTLFIGAMLGAAVGGAQHALLPHLKASPGTYALVGMGVL
                     FAGFLRAPMTSVFMVLEVSGNYSIILPVIVANTFAYVISRSLQPIPIFDVLTRQDGLE
                     LPSMEEQREEDILR"
     gene            complement(1393..2556)
                     /locus_tag="VEJ00_15475"
     CDS             complement(1393..2556)
                     /locus_tag="VEJ00_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXX02616.1"
                     /translation="MDTSQGSAAPANRTRLQSYLAREPVLLAALSLLAVASFLAVTGL
                     SRLIHAQQASLGQRWFDRGVADLGAQRYDRATQEFRAALRYSRDNYLYQLNLAEALIG
                     AKRTSEASAYLLNLWDRQPENGLVNLDLARIAAQQREIEQALRYYHNAIYATWPSDEE
                     VQRRETRVELIEFLLRLNAKEQAQSELIALAANAGEDAMQQVRIGDLFVRAQDYEHAL
                     AAYRVSLRSERHNAAALAGAGSAAFELGQYPTAYHYLQATVASNPRDTESADRLKTTE
                     LVLQMDPFQRQISVAQRNRLVVRAFQTAGERLKSCTSAADSSVSAAQGKLSDSWTKLN
                     PQISERGLQRDPDLVESAMHLVSTIERQTASACGPPSGADQALLLIAKQHEGN"
     gene            complement(2695..4557)
                     /locus_tag="VEJ00_15480"
     CDS             complement(2695..4557)
                     /locus_tag="VEJ00_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HXX02617.1"
                     /translation="MRKTTSGAEAQQKSIDSRGPKGPLFHQTTSAPAQFRIVLVSFLS
                     AAIGLVAGLVAYALYKLIGLFTNLFFFHEWSATFRSVGSHHLGAWVIAVPVIGGLIVG
                     VMAKYGTSKIKGHGIPEAMEAVLINRSRIAPRVALLKPISAAIAIGTGGPFGAEGPII
                     QTGGALGSLVGQILHTTAVERKTLLACGAAAGMSATFNTPIAGVILAIELLLFEFKSR
                     SFIPLVIASTLATAVHMQLLGPGPMFTVSAMDFGIPKALPFYLALGLVCGLAAVALSK
                     ALYWVEDAFEKLPWDELWWPAIGALALGLIGYKVPRVFGVGYDTIGDILNGALGWKLL
                     LIVMVAKFAALVISLGSGTSGGLLAPTFMWSAAMGGVFAMAGNYFFPSAHLSPGAFAL
                     VAMGAVFGAASRATFSFIIFAFEITRDYNSVLPLMLVAVIADGIAMLFMPHSSIMTEK
                     LSRRGLRVHQDYEADALTQATVGETMEKEPPLIPASMKVGELADRVAQHDPAIGRHAG
                     MLIVDDAGKLVGIITRGDMVRAIDKDSSGAMTVQEAGSTHLVVTYPDELVSEAAAKLL
                     RFDIGRLPVVERGDERKVVGYLGRAAILAARLRRFQDEHVLERGWLPAFSKSPE"
     gene            complement(4580..5020)
                     /locus_tag="VEJ00_15485"
     CDS             complement(4580..5020)
                     /locus_tag="VEJ00_15485"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXX02618.1"
                     /translation="MKRLALSDYQALAEFRFQIRKFLHFSEQAVQAAGLERGQYQLML
                     AIKGIPEGVRPRIRELANRMQIQHHSAVELINRLERGGFVRRERAQDDRREVLLALTP
                     KGERVLGELAVHHHDELQSAAPGLVAALRRVMRGGSGRTSLGRE"
     gene            5246..6592
                     /gene="purB"
                     /locus_tag="VEJ00_15490"
     CDS             5246..6592
                     /gene="purB"
                     /locus_tag="VEJ00_15490"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXX02619.1"
                     /translation="MIPRYTRPEMARIWSDENRFRTWLAVEVAATETLAEAGLVPKDA
                     ARAIKERADFRVERIHEIEAEVRHDVIAFTTAVAEIVGPHARWFHYGLTSNDVVDTAQ
                     ALLIRQASQVIAQDLQKLSDVLERRAWEFKDTPMIGRTHGIHAEPITFGFKIANWYSE
                     TQRNIARFLAAAEDMRVGKFSGAVGIFAHLTPELEEKICARLGLKAAAVSSQVIQRDR
                     HAHYLGTLAVIASTLDKIATEIRHLQRTEVREAEEFFSEKQKGSSAMPHKRNPVTAEQ
                     ISGLARVVRSNAQAGFENVALWHERDISHSSVERVILPDSTTLADYLLTKTANLVDTM
                     FVYPDRMRANLESTRGLIFSGQLLLDLVEHGVSREDAYRLVQSHAMRAWKEGLDFHQA
                     VLADKEITSRVPRQQIEHAFNLQRQLKNVDKIFARVFGPAQAKKTARKTGRIKPRV"
     gene            6771..7073
                     /locus_tag="VEJ00_15495"
     CDS             6771..7073
                     /locus_tag="VEJ00_15495"
                     /inference="COORDINATES: protein motif:HMM:NF017897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HXX02620.1"
                     /translation="MLGNRTSEQWIAQYATSHQHPVNRFCHTFGIPLILVSVAVFITS
                     FFVHRLWLYGLSLFVIGWIFQFVGHAFEGKPPEFFQDWRFLFVGVRWWWAKIRGRA"
     gene            7282..7668
                     /locus_tag="VEJ00_15500"
     CDS             7282..7668
                     /locus_tag="VEJ00_15500"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXX02621.1"
                     /translation="MFPQLARFTDLGLLLLRLMVGLTFATSGYTHLKDPEARAKSIGM
                     PKSFTIFLGLAEVAGALGVAFGVLTQLAAFGLILIMLGAIQKKIFAWHTGFWGEKTYG
                     WHYDLMFILMNLVIAFTNGGAWVLMK"
     gene            7942..8403
                     /locus_tag="VEJ00_15505"
     CDS             7942..8403
                     /locus_tag="VEJ00_15505"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HXX02622.1"
                     /translation="MKRFTPFLYCTLTAFAAVAPFYVAPPLAVAQQQKSLYQRLGGYD
                     ALAAVTDDFLGRLATDPQLKRFFIGHNKEGLTRIRQHVIDFLCVATGGPCAYTGQDMK
                     TAHTGLGITEDDWNASVKDLIATLNKFNVPEKEKNEVLSAISSLHGDIVGR"
BASE COUNT         1698 a         3036 c         2380 g         1520 t
ORIGIN      
        1 cgcagaatgt cttcctcgcg ctgctcctcc atcgagggca gttccagccc atcctgccgc
       61 gtgagcacat cgaagatcgg gatcggctgc aggctgcgcg aaatcacgta ggcaaacgta
      121 ttggccacga tgaccggcaa aatgatcgaa taatttccgc tcacctccag caccatgaac
      181 accgaggtca tcggcgcacg cagaaatccg gcaaacagca cccccatgcc caccagcgcg
      241 taagttcccg gcgatgcctt gagatgcggc agcagcgcat gctgcgctcc gcccaccgcc
      301 gcgcccagca tcgcgccaat gaatagcgtc ggagcgaaca ttcctcccgg cgttccgctc
      361 acgaacgaaa gcatggtcgc gagaatcttc agtcctgcca ggatggctag gaactgccac
      421 gtaaaccgcc cgtgcatggc ctcgtcaatg tattcgtatc ccgcgcccat gacctgcggc
      481 gcgcccagca ccgcaatcat tccgatgagc agccccgcca gcgccggctg gaaatactgc
      541 gtccagcggg gcagcgcctt gcagcgcgga cgcagggtcg taatccccgc cgagaacatc
      601 accgacgcca gcccgccaac caggcccagg acggaatacg ccagcaactc cgatggccgc
      661 cgcatctcga ctgcgggaat gcgaaacaat gattccgatc ccagaaacca gcgcatcacc
      721 accacgctcg acaccgccga cagcaccacc gatcccagaa tgcccgccgt ccaccgcccg
      781 atcacttcct cgatcacgaa cagcaccgct gaaatcggcg cattgaaagc cgccgccagt
      841 ccggccgccg cgcccaccgg cgcaatcagc cgcatgcgat cccgcgacag attcatccgc
      901 cgccccagcg ccgacgccag gctcgcgccg atctgcagcg atggatcttc cggacccaaa
      961 gagtgtcccg acccaatggc caacgccgcg gtgatgaatt tccccacggc cgtgcgcagc
     1021 ggaatgtacc cgttgtagat atacagcgcc gccttggttt gattaacgcc gctgccgcgc
     1081 gccagcggga aaactctcat taccagcgcg gcgatgacca accctgccag cgtgggcgcc
     1141 agcaccagcc gcgctggcgt aagaacgatc cccgatccca gcagataaat ccggcaccag
     1201 tcgatcgcga agcggaaaca gaccaccgcc agccccgaga acaccccgat gaagaccgac
     1261 agcacgagga agaatcggtc ttcccggaac accgaccaca acagagcctt gggagagcga
     1321 tcgaacagcg cggccagctt ctcctggcca accgccggtg cggcgctggt ttgtgtcgtg
     1381 ccggtgttca cgctagtttc cctcatgctg cttcgcaatc agcaacaggg cctggtccgc
     1441 cccagaagga ggcccgcagg ctgacgcggt ctggcgttca atggtagaaa caagatgcat
     1501 cgccgattcc accaggtccg gatctcgttg caggccgcgc tcgctgatct gcgggttcag
     1561 cttcgtccag ctgtcgctca gtttgccttg cgccgccgaa acgctggaat cggccgcgct
     1621 tgtgcacgac ttcaggcgct cgccggccgt ctgaaacgcg cgaactacca gccggttgcg
     1681 ctgcgccacc gagatctgcc gctgaaacgg gtccatctgc agcacgagtt ctgtggtctt
     1741 gagccggtca gcgctctcgg tatctcttgg attgctggcc acagtagcct gaagatagtg
     1801 ataggcggtc ggatattgcc ccaactcgaa cgccgccgat cccgctccgg ccagcgcggc
     1861 cgcgttgtgg cgctcagacc ttaagctgac gcgatacgcg gccagcgcat gctcatagtc
     1921 ctgggcacgc acgaacaaat cgccgatgcg tacctgttgc atcgcgtcct cccccgcatt
     1981 cgccgccagg gcgatcaact ccgactgcgc ctgctccttg gcattgagcc gcaacaggaa
     2041 ttcgatgagt tccacccgcg tctcgcgccg ctggacttcc tcatcgctcg gccacgtcgc
     2101 atagatcgcg ttgtgatagt aacgaagcgc ctgctcgatt tctctctgtt gcgcggcgat
     2161 ccgcgccagg tccaggttca ccagcccgtt ctccggctgc cggtcccaaa ggttgagcag
     2221 ataggcggag gcttcactcg tgcgcttcgc tccgatcagc gcctcggcca gattcagctg
     2281 gtaaagataa ttgtcgcggg aatagcgcaa tgcggcccga aattcctgcg tggcccggtc
     2341 gtagcgctgc gcgcccagat cggcgacgcc gcggtcaaac caccgctgcc cgagagatgc
     2401 ttgctgggca tgaatgaggc gcgacagccc ggtcacagcc agaaacgacg ccaccgccag
     2461 cagcgaaagc gcggccagca gcaccggctc gcgggccagg taagactgaa ggcgcgttcg
     2521 gttcgctggg gcggcgctgc cttgcgaggt atccatcgag tctcttctga tgatacccgg
     2581 cgcaggcacg cgaatccggc catccccgtg gaagagcggc gcttcagggc cgcgctcagt
     2641 ctccagtcgt gaggggcttt agctccggtg tcgtcttccc cgaacgaacc cgttctattc
     2701 cggactcttg gaaaacgctg gaagccaccc ccgctcgagc acgtgctcat cctggaagcg
     2761 ccgcagccgc gccgcgagaa tcgcggcccg ccccaggtag ccaaccacct tgcgctcatc
     2821 gccacgctct accaccggca atcgcccgat atcgaagcgc agcagcttgg ctgccgcttc
     2881 cgaaaccagt tcgtccggat acgtcaccac aagatgcgtg ctacctgcct cctgtaccgt
     2941 catcgcacca gaagaatcct tgtcgatcgc acgcaccatg tcgccacgcg tgatgatgcc
     3001 gacgagtttg cccgcgtcgt cgacgatgag catgcccgca tgtcgtccga tcgccggatc
     3061 gtgctgcgcc acccggtccg ccagctcccc caccttcatg ctggccggaa tcaggggagg
     3121 ctccttctcc atcgtttcgc cgacggtagc ctgcgtcagc gcgtcggctt cgtagtcctg
     3181 atgcacccgc agcccgcgcc gcgacagctt ctcggtcatg atggagctgt gcggcataaa
     3241 caacatggca atgccgtccg cgatcaccgc caccagcatc agcggcagca ccgagttgta
     3301 atcccgtgtg atctcaaacg cgaagatgat gaacgaaaac gtcgctcgcg acgccgcccc
     3361 gaagaccgct cccatcgcta ccaatgcgaa cgcgccgggg gataggtgcg ccgaaggaaa
     3421 gaaatagttc ccggccatgg cgaacacgcc gcccatggcc gcgctccaca tgaaagtcgg
     3481 cgccagcagg ccccctgacg ttcccgaacc cagcgaaatc accagcgcgg caaacttcgc
     3541 taccatcacg atcagaagca atttccaccc gagagctccg ttcagaatgt cgccgatcgt
     3601 gtcgtacccg acgccgaaaa ctcgcgggac cttgtatccg atcagtccca gcgccagcgc
     3661 cccgatcgcc ggccaccaca gctcatccca tggcagcttc tcgaacgcgt cttccaccca
     3721 gtacagcgcc ttgctaagcg caaccgccgc aagcccgcag accagaccca gcgccaggta
     3781 aaacgggaga gctttcggaa taccgaagtc catcgccgac acagtaaaca tcggccccgg
     3841 ccccagaagc tgcatgtgta ccgccgtagc cagcgtgctt gcaatgacca gcggaatgaa
     3901 cgatcgcgac ttgaattcga acagcagcag ctcaatcgcc agaatcacgc cggcgatcgg
     3961 agtattgaac gtcgccgaca ttccggccgc ggcgccgcag gccagcagcg tcttccgttc
     4021 caccgccgtc gtgtgcagaa tctggcctac gagtgacccc agcgccccgc ccgtctggat
     4081 gatcggtcct tccgccccga acggcccgcc ggtcccgatc gcaatcgccg cggaaatcgg
     4141 cttgagcagc gccacccgcg gcgcgatccg gctgcgattg atcagcaccg cttccatcgc
     4201 ttccggaatg ccatgccctt tgatcttcga cgtcccatac ttggccatca cgcccacgat
     4261 cagcccgccg atcaccggga ccgcgatgac ccaggcgccg aggtgatgcg acccaacgct
     4321 gcggaaagtc gccgaccact catggaagaa gaagagattg gtgaacagcc cgatcagttt
     4381 gtaaagagcg tacgccacca gcccggcgac cagcccgatc gccgccgaca aaaacgaaac
     4441 cagcacgatg cgaaactgcg ccggtgcgct ggtggtttgg tggaagagcg gccctttagg
     4501 gccgcgtgag tcgatcgact tttgttgggc ttccgcccct gaggttgttt ttcgcattgg
     4561 aaacatgttc ttgagaggct tactcgcggc caaggctcgt tcgcccggac cctccgcgca
     4621 tcacccgccg caacgccgct accagcccag gagccgcgct ctgcagttcg tcgtgatgat
     4681 gcaccgcgag ttcccccagc acccgctcgc ctttcggcgt aagcgccagc agcacttcgc
     4741 gccggtcgtc ctgcgcccgt tctcgccgca cgaacccgcc ccgctccagc cggttgatga
     4801 gctccaccgc gctgtgatgc tggatctgca tccggttggc cagctcgcga atgcgcggac
     4861 gcaccccctc cgggatgccc ttgatcgcca gcataagctg gtattgcccg cgctccagac
     4921 ccgcagcttg caccgcctgc tcgctgaaat gcagaaactt tcgtatttga aaccggaact
     4981 cggctaatgc ttgataatcc gacagggcga gcctcttcat gctgccgatt gtatcgtgtc
     5041 gcgatagtcc aagattcatt atatcgtgat acgacatata acgaaaggga ttctgtgctg
     5101 ttgatcccac cgtggttgat cccaccgtga aagagcggcg cttcagcccc gcgtcaggcg
     5161 tcgggttgaa cggggcgtta gcccccgtgg tctagcctct ttccttccct ccatttagaa
     5221 tcctctgtcc gtcacaggag cctccgtgat cccgcgttac acccggcccg agatggcccg
     5281 catctggagc gacgaaaacc gcttccgcac ctggctcgcc gtcgaagtcg ccgccaccga
     5341 aacccttgcc gaagccggcc tcgttcccaa ggacgcggcc cgggcgatca aagagcgcgc
     5401 cgacttccgc gtcgagcgca tccacgaaat cgaggccgaa gtccgccacg acgtcatcgc
     5461 cttcaccacc gcggtcgccg aaatcgtcgg cccgcacgcc cgctggttcc actacgggct
     5521 gacctccaac gacgtcgtcg ataccgcgca ggccctgctc atccgccagg ccagccaggt
     5581 catcgcgcaa gacctgcaaa agctttccga cgtcctcgag cgccgcgcct gggagttcaa
     5641 agacacgccc atgatcggcc gaacacacgg cattcacgcc gagccgatca ccttcgggtt
     5701 caaaatcgcc aactggtatt cagagacgca gcgcaacatc gcgcgcttcc tggctgcggc
     5761 ggaagacatg cgcgtgggta agttttccgg cgcggtcggc atcttcgcgc acctcacgcc
     5821 ggagctcgaa gagaaaatct gcgcgcgcct gggattgaaa gccgcggccg tgtcgtcgca
     5881 ggtgatccag cgcgaccgcc acgcgcacta tttgggcacg ctggccgtga tcgcatctac
     5941 gctggacaag atcgcgactg agattcgtca cctgcagcgc accgaagtgc gcgaggccga
     6001 ggagttcttc agcgagaagc agaaaggctc gtcggccatg ccgcacaagc gcaatccggt
     6061 gacggccgag cagatcagcg ggctggcgcg cgtggtgcgg tcaaacgcgc aggcgggatt
     6121 cgaaaatgtg gctttgtggc acgagcgcga catctcgcac tcgtcggtcg agcgggtcat
     6181 cctgcccgac tcgaccacgc tggccgacta tctgctcacc aagaccgcga acctcgtcga
     6241 caccatgttc gtctatcccg accgcatgcg cgccaatctg gagtcgacgc gcggcctcat
     6301 cttcagcggg cagctgctgc tcgacctggt cgagcatggc gtcagccgcg aggacgccta
     6361 tcgcctggtg caatcccatg ccatgcgcgc gtggaaagag ggtctcgact ttcaccaggc
     6421 cgtgctcgcc gacaaggaga tcaccagccg cgtgccgcgc cagcagatcg agcacgcctt
     6481 caacctgcag cgccagctca agaacgtgga taagatcttc gcccgcgtct tcgggcctgc
     6541 acaagccaag aaaaccgctc gcaagacggg ccgaataaaa ccgcgtgtgt agtgcacaag
     6601 atgctccccg cctatcacaa tctcggcaag ccccctactt caccgacgaa tacgaatact
     6661 cctccggctt tgccaccaac cgcgcccgca ccggattgag ccggagtcga gcaccgcgtt
     6721 cgagtaccag aaatgacggc gccttggccc gggtgtatga ttctcaacgc atgcttggca
     6781 accgcaccag cgagcaatgg atcgcccaat acgcgaccag ccaccagcac cccgtcaacc
     6841 gcttctgcca caccttcggc attcccctga tcctcgtctc ggttgccgta ttcatcacca
     6901 gcttcttcgt ccatcgcctc tggctctacg gtctctcgct gttcgtcatc ggatggatct
     6961 tccagttcgt cggccacgcc ttcgaaggca agccccccga gttcttccag gactggcgat
     7021 tcctttttgt cggcgtccgt tggtggtggg ccaagatccg cggccgcgcc tgaccagtgt
     7081 ggcgcggaca ttcctgtccg cgtctgcgcg tgccggcgga actcaagtta aggagcaccg
     7141 cggcagcaca tctgcatctc aatcccaacc gcgcagccgc ggaattcatc agcccggcac
     7201 gtgagtgccg gcaaaagcgg taggatggat gcgagtcccg cagggacggc gctgttccgc
     7261 tgcgcgcacc aggagctccg catgttcccc caactcgccc gcttcaccga tctcggcctc
     7321 ttgctcctgc gcctcatggt cggcttgacc ttcgccacca gcggctatac tcacctcaaa
     7381 gatcccgaag cccgcgcgaa aagcatcggg atgccgaaat ccttcaccat cttcctcggc
     7441 ctcgctgaag tcgcgggcgc gctcggcgtc gccttcggcg tcctgaccca actcgccgcc
     7501 ttcggcctga tcctcatcat gctcggggcc atccagaaga agatcttcgc ctggcacacc
     7561 ggtttctggg gcgagaaaac ctacggctgg cattacgacc tgatgttcat cctcatgaac
     7621 ctggtcatcg ccttcacgaa cggcggcgcc tgggtactaa tgaagtaaag cgaatcgcta
     7681 gcacgcccct ctcgcacccc gagattttct ttgcgctttc tgcggctttt cctcagcgat
     7741 tttgtgttca aaaagctttt gccgcagagt tcgctatgga aaagccgcaa agaacgcaaa
     7801 gcaaccagct accggatccc gggccaccga attttccctt ggcgacctcc cccatcaaga
     7861 acggtaatgt cggcaatccg tttcgactgc cccgccgtac ttccacacga ggcttaaatc
     7921 cattccgttt caggagacac catgaagcga ttcacgccgt ttctgtattg caccctgacc
     7981 gccttcgccg ccgtcgcgcc cttctacgtc gcgccgccgc tggccgtcgc tcaacagcag
     8041 aaatctcttt accagcgcct cggtggatac gacgccctgg ccgccgtcac cgacgatttt
     8101 ctcggccgcc tcgccaccga tccccagctc aagcgcttct tcatcggaca caacaaggaa
     8161 ggcctgaccc gcatccgcca gcacgtcatc gacttcctct gcgtcgccac cggcggcccc
     8221 tgcgcctaca ccggacagga catgaaaacc gcccacaccg gcctcggcat caccgaagac
     8281 gactggaacg cgtcggttaa agacctgatc gccacgctca acaagttcaa cgtcccggaa
     8341 aaagagaaga acgaggttct cagcgccatc tccagcctgc acggcgacat cgtcggacgc
     8401 tagccgtccc attgaatacg ggtgccccat ttctcgcgcg tcctttacgc gagaagtggg
     8461 gatttctatg gaaccctgcc ccgtgcgcac tacccgcggt tgtcccactc ttctcgcagc
     8521 gggtacccca tccctaacgt cgtgctcttt gcgaggctag gattgggggg ttgacgttgc
     8581 caccctgctc gcgttctgtg cgagagggtg gggcatccag agggactgaa aacg
//
LOCUS       DASPUL010000674         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1633412, whole genome shotgun sequence.
ACCESSION   DASPUL010000674 DASPUL010000000
VERSION     DASPUL010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633412"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VEJ00_15510"
     CDS             <1..508
                     /locus_tag="VEJ00_15510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXX02623.1"
                     /translation="AIYVPADDYTDPAPAATFTHLDATTNLSRQIVERGIYPAVDPLA
                     STSRILDPRIVGQEHYDVAQAVKKTLQTYKDLQDIIAILGIDELSEDQKLTVARARKI
                     QRFLSQPFHVAEQFTGAKGKYVKIAETVKGFKAIVEGKYDDIPEQAFYMKGPIEEVLE
                     AAEKMKAV"
     gene            572..979
                     /locus_tag="VEJ00_15515"
     CDS             572..979
                     /locus_tag="VEJ00_15515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HXX02624.1"
                     /translation="MADTFQLEIVTPEKKVVDTAAEEVQIPGKNGYLGIMPGHAPLIT
                     ELAVGEITFREKSHEQRLAVAWGFAEVLPDKVTVLAETAERPSEIDVERARKAKERAE
                     QRLTSGDTSVDVERALDALQRAEVRLHVATESK"
     gene            1181..>2237
                     /locus_tag="VEJ00_15520"
     CDS             1181..>2237
                     /locus_tag="VEJ00_15520"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXX02625.1"
                     /translation="MRIGTRVAWLVLFFLPSFAVSQDAKRTLTRPEPRPALVSPEVHS
                     DGSVTFRFPAPNALEVKLDREGAESVSMKKDEGGVWNVTTAPLSPDYYGYSFVADGVR
                     LIDPSNPLLVPNLLDPQSAVHVPGPAALPWEVNDVPHGEIHHHFYRSVVAGDDRDYYV
                     YTPPGYSPAAKQTYPVLYLLHGFSDDASGWTAVGRANVILDNLIAQGKAKPMIVVMPL
                     GYGTMEMVQAGWNAWNHAELRNENFTKFSEALLKEVMPRVESEYRVKSGREARGIAGL
                     SMGGSESLLTGLNHLDKFAWIGAFSSGGLTGDFQTDFPALDAKANQQIRLLWIACGTE
                     DHLITVNRNFREWLKSKG"
BASE COUNT          461 a          633 c          735 g          408 t
ORIGIN      
        1 ggcgatttac gtgcccgcag acgactacac ggatcccgcg cccgcggcca cgttcacgca
       61 cctggacgca accaccaacc tcagccgcca gattgtggag cgaggcattt atcccgcggt
      121 cgatccgctg gcctcgacct cgcgcattct cgatccgcgc attgtgggcc aggagcacta
      181 cgacgtggcg caggcggtga agaaaacgct gcaaacctat aaagacctcc aggacatcat
      241 cgcgattctc ggcatcgacg aattgagtga agatcagaag ctgacggtcg cgcgcgcccg
      301 caagatccag cgcttcctct cccagccgtt ccacgtggcc gagcagttca ccggtgcgaa
      361 gggcaagtac gtcaagatcg ccgaaacggt caagggattc aaagcgatcg tggaaggcaa
      421 gtacgacgac atccccgagc aggcgttcta catgaaaggg ccgatcgagg aagtgctgga
      481 agcggcggag aagatgaagg cggtgtaaag gacggctctc ggccttcggc ttccggcttt
      541 cgcggaagct gagcgccgga agctgatttc aatggcagat actttccaac tcgagatcgt
      601 cacgccagag aagaaggtcg tggacacggc tgcagaggaa gtgcagattc cgggcaagaa
      661 cggatatctc gggatcatgc cggggcatgc gccgctgatc acggagttgg cggtcggcga
      721 gattaccttc cgcgagaagt cgcacgagca gcggcttgcg gtggcctggg ggtttgcgga
      781 agtcctgccc gacaaggtca cggtgctggc cgagaccgcg gagcggccat cagagattga
      841 tgtggagcgc gcgcgcaagg ccaaggagcg cgccgagcag cggctgacga gcggcgacac
      901 gagcgtagat gtggagcgcg cactggatgc gttgcagcgg gccgaagtgc ggttgcacgt
      961 ggccacggaa agcaaatagc cagcgctgtg ctgcttcgac cagcgaaata cgaccacagg
     1021 ggctgaagcc ccatgtttct cggcacttca cgcggcgctg aagcgccgct cttccatgtg
     1081 cgtgcgacgc tcggaatgtg attcgggcgg gcgctcaacg ggctttcaca gcggtggcac
     1141 gcgaagtagc tgtccacgag actccgatct ggagtagctg atgagaatcg gtactagagt
     1201 tgcttggctc gtcctcttct ttctgccatc gtttgccgtg tcacaagatg cgaaacgcac
     1261 gcttactcga cccgaaccgc gcccggcact ggtctcgccc gaggtccaca gcgacgggag
     1321 tgtgactttc cgcttcccgg ctccgaacgc gctcgaagtg aagctggacc gcgaaggcgc
     1381 cgagtcggtt tcgatgaaga aagacgaggg cggcgtatgg aatgtgacga ccgcgccgct
     1441 gagtcctgat tattacgggt actcgttcgt ggctgatggg gtgcgtctga tcgatccttc
     1501 aaacccgctg ctggtgccga atctgctcga tccgcaaagt gcggtgcatg tccccggccc
     1561 cgcggctttg ccctgggaag tgaatgacgt accgcatggc gagatccatc accatttcta
     1621 ccgctccgtg gtcgcgggcg atgatcgcga ctattacgtc tatacgccgc ccggctacag
     1681 tccggccgcg aagcagacct accccgtgct ttatctgctc cacggattca gcgacgacgc
     1741 cagcggatgg accgcggtcg gccgggcgaa cgtcattctc gacaacctga tcgcgcaggg
     1801 caaggcgaag cctatgattg tggtgatgcc tctcggctat ggaacgatgg agatggtgca
     1861 ggcggggtgg aatgcctgga accatgccga gttgcgcaat gagaatttta cgaaattcag
     1921 cgaagcgttg ctcaaggaag ttatgccgcg agtggaaagc gagtatcggg taaagagcgg
     1981 ccgggaggcg cgggggattg cggggctttc gatgggcgga tcggaatcgc ttttgaccgg
     2041 cctgaaccat ctcgacaagt tcgcctggat cggggccttc agttcgggcg gtctgaccgg
     2101 agatttccag accgacttcc ccgcgctgga cgcgaaggcg aaccagcaga ttcggctgct
     2161 gtggatcgcc tgcgggactg aagatcatct gatcacggtg aatcgcaact tccgcgagtg
     2221 gttgaagtcg aagggca
//
LOCUS       DASPUL010000675         4239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_132252, whole genome shotgun sequence.
ACCESSION   DASPUL010000675 DASPUL010000000
VERSION     DASPUL010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132252"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VEJ00_15525"
     CDS             complement(<1..883)
                     /locus_tag="VEJ00_15525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXX02626.1"
                     /translation="MWSAIRRPGLHPMALLVAASLLGTLACSSTTPVTQAATPSAAGK
                     PEVTSAAPAQKPAQKDDGDYVASGPIIVENQVDVLALTGGVVAEIVADVGSNVHKGQL
                     LARLDDRQATAQRDAAQAKLKSSEADLADWQAETKMAEADYDRAQRMRESGINTQEAL
                     DHARYKLVGSQYEIEKAQREIDSARDSLRISELELQKTRIEAPFDGVVARRYVRAGQT
                     AAPGDRMFWVSAVGPLLVKFTLPERFLNQVKTGNRVYVTSLLAPGQAQPARIVQMSPL
                     VDPASDSIDVVAKLEGKP"
     gene            complement(908..3307)
                     /locus_tag="VEJ00_15530"
     CDS             complement(908..3307)
                     /locus_tag="VEJ00_15530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXX02627.1"
                     /translation="MARQTTTFAISVTELAAGLLAQREVVPRAQFTADQTAQLLPGTS
                     VTVYVIPDQENPVWTAKASTGEVACAAEVEFHAGTLGALAETRSTVLLKGSEVPRESY
                     SHLDTRRQVAFLAYVPLLVDATLIGAIEMVQHEESFPEAMLDAVHEIAHLASPALAAS
                     LNYESERNASLQSISRVTQMYDLEKVFNSTLEMDELLAIIAKKFQEMMGVQGINLWMI
                     NQDLPELLNQAGWDPTVEIGAAQKPGEGVVGDISDTGESVLIADPEDERLKKRNAGRE
                     EGTVFSLLAVPLLEHEKLVGVVEAVNRVDGAPFDEDDQFLFINICETASNALHNASLL
                     QAERKVEILQTLVRISGEITSTLNLDRVLQAVVNGPSTVIPHERSAIALEQRGRLQLK
                     ALSGMEQINPEAPDVDRLNRILQWASMLNEELFVSQTGDEVKAAREETREKFHRYFAE
                     SGMRAFYAVPLLDDEGRLGILSFESSDPDFLTDAHLEMIKVIAGQATVALRNASLYQE
                     VPFIGVLEPLLQRKAKFMALPGRRRAAILTMAAAAVIFLGAVPMPMRVEGSAVVAPAR
                     KAQIQPEVDGVVRNVYVHEGDSVEQGSVVADLEDWEYRGALSAAQAKYESAQSEANRA
                     LAANDGEQAGIQRVAAQYWAAEVRRLQERWEKTRLRSPISGKVLTPHPENLVGRKLTA
                     GDEFIEVADSSQAVIDVAIDESDIPLVRTGTGAAVKLESYPQRTFHGAVGVVSPQGQV
                     EQDERVFYARVNVPNTDGLMRPGMQGRGKVSVGWRPVGVVFLRRPVMWIYSQLWSWLG
                     W"
     gene            3689..>4239
                     /locus_tag="VEJ00_15535"
     CDS             3689..>4239
                     /locus_tag="VEJ00_15535"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HXX02628.1"
                     /translation="MSARIGRISLRIAIALFLTLACSAAQTANPPIYLDPSQPIPDRI
                     DDLLQKMTLEEKASQLVNQARAIPRLQIPAYDWWSEALHGVANAGTATVFPEPIGLAA
                     TFDDPLVHDVAAAIGTEARAKHNQAVKAGRRDMMEGLDVWAPNLNIFRDPRWGRGQET
                     YGEDPFLTARMGVAFVTGLQGDDP"
BASE COUNT          780 a         1334 c         1247 g          878 t
ORIGIN      
        1 caggcttgcc ctccagcttc gccaccacat caatggaatc gctcgccggg tcgacgagcg
       61 ggctcatctg cacgatcctg gcgggctgag cttgaccggg tgccagcagg gaagtgacgt
      121 agaccctatt gccagtcttg acctggttca ggaatcgctc gggtagcgtg aacttcacca
      181 gaagcggacc taccgccgag acccagaaca tgcggtcgcc cggcgccgcc gtctggccgg
      241 cgcgcacgta gcggcgtgcg accacgccat cgaagggggc ctcaatacga gtcttctgca
      301 gctccagttc ggaaatccgc aggctgtcgc gcgcgctgtc gatctcgcgc tgagcttttt
      361 caatttcgta ttgcgacccg acgagtttgt agcgagcgtg gtcgagcgct tcctgcgtgt
      421 tgatccccga ttcccgcatc cgctgggcgc gatcgtaatc ggcctcggcc atcttggtct
      481 cggcttgcca atcggcaagg tcggcctcgg aactcttcag cttggcttga gcggcgtcgc
      541 gctgagccgt tgcttggcgg tcgtccaggc gggctaatag ttggcccttg tgaacgttcg
      601 aacccacgtc agcgacaatc tcggcaacaa ctccgccggt caaggccaga acatccacct
      661 ggttttcgac gatgatgggt cccgaagcga cgtagtcgcc atcatctttt tgcgcgggct
      721 tttgagccgg cgcggcggat gtgacttccg gcttgcctgc cgcgctcggc gtggcggcct
      781 gggtaactgg agtagtgctg ctgcatgcca gagttcccaa tagacttgcc gcaaccagca
      841 acgccattgg gtgcagaccc ggccgacgaa tggctgacca catctccgcc tccctctcta
      901 aagatcttta ccatcccaac caagaccaca gctgggaata gatccacatc accggacgcc
      961 gcagaaatac cacgccgacg ggacgccatc caaccgagac cttccctcgc ccctgcattc
     1021 cgggccgcat caatccgtcg gtgttcggca cattcacccg cgcgtagaag acccgctcgt
     1081 cctgctcgac ttgaccctgg gggctgacca cgcccaccgc gccatggaag gttctctggg
     1141 ggtagctctc aagcttgacg gcggcgccgg ttccagtgcg gacaagaggt atgtcgctct
     1201 catcaatcgc aacatcgatg acggcttgcg agctgtcggc gacctcgatg aactcatcgc
     1261 ccgctgtgag ttttcgcccg acgagattct cggggtgagg cgtcaggact ttgccgctaa
     1321 tggggctgcg cagccgcgtc ttctcccacc gctcctggag tctccggacc tcggctgccc
     1381 aatactgcgc ggccacgcgc tggatgcctg cctgctcgcc gtcattcgca gccagggcgc
     1441 gattggcctc ggactgtgcg gactcatact tggcctgggc cgcactcagc gccccacggt
     1501 attcccaatc ctcgagatcg gccacgacgg agccttgctc gacagaatcg ccctcatgca
     1561 cgtagacgtt gcgcacgact ccatccactt cgggctgaat ctgggctttc cgggcgggcg
     1621 cgaccactgc gctgccctcg actctcatcg gcatgggaac cgctccgagg aatatgactg
     1681 cggcggcggc catggtgaga attgccgcgc gccttcgtcc cggaagcgcc ataaacttcg
     1741 ccttccgctg cagaaggggc tccagcactc caatgaaggg tacttcctga tagagcgatg
     1801 cattgcgcag ggctaccgtg gcttgcccgg caatcacctt gatcatctcg aggtgagcgt
     1861 cagtcaggaa gtcgggatcg ctgctctcga acgaaaggat gcccagccgt ccttcgtcgt
     1921 cgagcagtgg gactgcatag aatgcgcgca tgccgctttc cgcaaaatag cggtgaaact
     1981 tctcccgcgt ttcttcgcgc gctgccttga cttcgtcccc ggtctggctg acgaagagct
     2041 cctcgttcag catggaggcc cattgcagaa tccgattgag tcggtcgacg tcgggagcct
     2101 ccgggttgat ctgctccatt ccggaaagag ctttcagctg cagccgccca cgctgttcca
     2161 gggcaatcgc cgagcgttca tgaggaatca ccgtgctcgg cccgttgacc accgcctgca
     2221 ggactcggtc cagattgaga gtggaagtaa tctccccgct gatccgaacc agagtctgca
     2281 ggatctccac cttgcgctcc gcttgcagca agctggcatt gtgcagcgcg ttgcttgcgg
     2341 tctcacagat gttgatgaac aagaactggt cgtcttcgtc gaacggggcg ccatccaccc
     2401 ggttcaccgc ttctaccacg cccaccagtt tctcgtgctc aagtagcgga accgcaagga
     2461 gtgagaacac cgtgccttct tcgcggccgg cattccgctt tttgaggcgt tcgtcttcgg
     2521 gatcggcgat cagcacgctt tcgccggtgt cagaaatgtc tccgacaacg ccttcgccgg
     2581 gcttttgcgc ggcgccaatc tcgaccgttg gatcccatcc tgcctgattc agcaactctg
     2641 gcagatcctg attgatcatc cacagattga taccctggac gcccatcatt tcctggaatt
     2701 tcttcgcgat gatcgccagc agctcgtcca tctccagggt cgagttgaag actttttcca
     2761 ggtcgtacat ctgggtgaca cggctgatag attgcaggct ggcattgcgc tcactctcgt
     2821 agttcagaga ggcagccagc gcaggagagg ccaggtgtgc aatctcatgg acagcatcca
     2881 gcatggcttc cggaaaagat tcttcatgct ggaccatctc gatggcgccg atcagcgttg
     2941 catccaccag cagcggaacg taggccagaa aggcgacctg gcggcgggta tccaaatggc
     3001 tgtagctctc gcgcggcacc tcactcccct tcagaagcac ggtgctgcgg gtctctgcga
     3061 gagctcccag ggtgccggcg tggaattcaa cctcggcggc gcaggcgact tctcccgtac
     3121 tcgcttttgc cgtccagacc gggttctcct ggtcggggat tacatagacg gtgacggatg
     3181 tgcccgggag caactgggca gtctgatcgg cagtgaactg cgcgcgcgga acgacctccc
     3241 gctgtgccaa caaacctgcg gcgagctcag tcacagaaat cgcgaatgtg gttgtttgcc
     3301 tcgccatgtt gcgagacttg ccggggaggg accctgggag cgccgggatc gtagcagttt
     3361 tgagacccgc gatccccagc gcccacgccg tcgagtgggc atcttatgct gctttcgggt
     3421 acaggtcaag attcgcaggt agcagaaaaa tgcatatgac tgatctattg ttcaaccgtc
     3481 aaggctttca tttccggggc ccaccatact gcgcacgcgc tcattgacgc tgcgaggatc
     3541 atggcaagca actcgctctc gttccgtgcc ggtccttttc gcattgccat tctgtaaact
     3601 atgtcgttca cgaaaagctt gggtgagagt ggagcgtacg gtccgattcg tgaagggccc
     3661 ggaccgtgcg caatcaagga gacggtgaat gagcgcacga atcggacgga tcagcctgcg
     3721 gatcgcgatt gcgttgtttc tcactctggc atgctcggcc gcgcagacgg ccaaccctcc
     3781 catctatctg gatccctccc agcccatccc agatcgcatc gatgatctgc tccagaaaat
     3841 gactcttgag gagaaggcat cgcaactggt gaaccaggcg cgggctatcc cccggttgca
     3901 gattcccgct tacgattggt ggagcgaagc gctgcacggc gtggccaacg cgggaacggc
     3961 caccgtattt cctgaaccga ttggattggc cgccaccttc gacgatccgc ttgttcatga
     4021 tgtggcggcc gccatcggca ccgaagcccg tgccaagcat aatcaggccg taaaggcggg
     4081 gcggcgcgac atgatggaag ggctggacgt ctgggcgccg aatctcaaca tctttcgtga
     4141 tccgcgctgg ggccgcggcc aggagaccta cggagaagat ccctttctca ccgcgcgcat
     4201 gggcgtcgcc tttgtgaccg ggttgcaggg agatgatcc
//
LOCUS       DASPUL010000676         4212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_405148, whole genome shotgun sequence.
ACCESSION   DASPUL010000676 DASPUL010000000
VERSION     DASPUL010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405148"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..1298)
                     /gene="purM"
                     /locus_tag="VEJ00_15540"
     CDS             complement(243..1298)
                     /gene="purM"
                     /locus_tag="VEJ00_15540"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HXX02629.1"
                     /translation="MAKIPPASAPTITYADAGVDIPRANRTKQRIKYLAHKTFTRGVL
                     SEIGGFGGLFAVDKTKYADPVLVSSVDGVGTKLKVAFAMDLHSTVGADLVNHCVNDIA
                     VQGAAPLFFMDYLATGKLDPAVAEKVVEGIADACKHNGCALIGGETAEMPGFYPDGEY
                     DLAGFIVGVVDRERIITGKDVQIGDIILGLPSNGLHTNGYSLARKLLFEVAHYSPETY
                     VNEIKNKVGNELMRTHKSYWPSLKKLIDAQCVVALAHITGGGITENLPRVLPRGTAAA
                     IELGSWPVLPIFEHLQQLGNVPQDDMLRTFNMGLGMLLVVPAAKFKKAQSVLERVGEK
                     AYTIGRIVKGERKVMYS"
     gene            complement(1584..1745)
                     /locus_tag="VEJ00_15545"
     CDS             complement(1584..1745)
                     /locus_tag="VEJ00_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02630.1"
                     /translation="MEDRAPVVSSTEQSSATTKQHEGRGAWVLTCYGICGLALFGVLA
                     YFFSEYISH"
     gene            1943..>4212
                     /locus_tag="VEJ00_15550"
     CDS             1943..>4212
                     /locus_tag="VEJ00_15550"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HXX02631.1"
                     /translation="MRNSSLFFLVCCLLAAGATAQQSGPTPAAQGSRPAAQSGSAQQS
                     GGASAAKPSASTADKAQDYSQESFVIEQLHSRYRFESDGTGRKEVVARIRVQSEAGVQ
                     QWGQVQIGYNSENERVEIPYVRVLKEDGNVVKAGDDAVQDLSAPFEREAPVYTDYRQK
                     HITVPGLRPGEVLEYDLVTVVHTPLAPGQFWTEYQFDKNSIVLDETVDVEVPSSRTLK
                     LKSKPGLDPAIREENGRRIYHWSSSHLVREDDKDKDKEKKKHHPDDDRPDIQLTTFES
                     WEQVGRWYAGLEKDRRVPSPEVRAKAAELTKGLTTDLDKTEGLYDFVAKNFRYVSLSL
                     GVGRYQPHASSDVLHNQYGDCKDKHTLLASLLEAEGLHASSVLINSSRKLDPDVPSPS
                     QFDHVITMLPLGKNEVWMDTTSEVAPFRLLAFTLRDKQALVIPPANPPSPVPPHLEET
                     PADTPMPDTELADIDGKINDIGKLEAHVHYEFRGDEELLLRSVFRRVPQANWQRVVEN
                     VNASLGGDITNLKVSDPAATREPFTLTYDVAKVNFLDWSKKKTEIGLPLVQFSLPDVD
                     DDTDADAEPLKLGPKAAYSYHIKLELPAKYTAHAPLAFALKRDYAEYEATYHLDSDVL
                     TAGRKLTLRQSELPAARAADYLSFRRAVESDLAQQVSMESSVAGTPVPPADMKADDLV
                     DSARAAADNGNLPVAIALLKRATEVDPKNKFAWNNLGLAYFQMREDAQAIAAFGKQIE
                     VNPYDEFAWNNLGRVY"
BASE COUNT          902 a         1294 c         1217 g          799 t
ORIGIN      
        1 aaggccaaag acgatccgct ggcgatggaa ccagggcatc ggtgttctcc tctcgtgcga
       61 ggtaccgttc atgatgaacg cgttcgaagg gtctgttgtt aaaggaatat gaaaatcaaa
      121 gaatcagcct tgaggtcctt ttcgccgtgc tctctgcggc cgttctctgc gttagatcgt
      181 cactcgcaga ggccgcggag acatcgcaga ggacgcggag agaatcggaa cccggggccc
      241 gttcagctgt acatcacctt gcgttcgcct ttcacgatcc ggccgatggt gtaagccttt
      301 tcacccacac gctccagcac agactgagct ttcttgaact ttgctgcagg aacgaccagc
      361 agcatgccca agcccatatt gaaggtgcgc agcatgtcgt cctgcggcac attgccgagc
      421 tgctggaggt gctcgaagat gggcaggacg ggccacgatc ccaattcgat cgctgccgcc
      481 gtcccgcgag gcaatacgcg cggcaggttt tccgtaatcc cgccgccagt gatgtgagcc
      541 agtgcgacga cgcattgcgc gtcgatgagc tttttcagcg acggccaata actcttatgc
      601 gtacgcatca gctcgttgcc gaccttgttc ttgatctcat tgacgtaggt ttcaggggag
      661 tagtgcgcca cttcaaacag cagcttgcgg gcgagcgaat atccgttggt gtgcagccca
      721 ttcgagggca atcccagaat gatgtcaccg atctgaacat ccttgccggt gatgattcgc
      781 tcgcggtcga ctacgccgac gatgaagccc gccagatcgt actcaccgtc gggatagaag
      841 ccgggcatct cggccgtctc gcctccgatg agcgcgcacc cgttgtgctt gcacgcgtcg
      901 gcgatgccct ccaccacttt ttcggcaacc gccggatcga gcttgcctgt agccaggtaa
      961 tccatgaaga aaagcggtgc agcgccttga acggcgatgt cattcacgca gtggttgacc
     1021 aggtctgcgc ccaccgtgga gtgcaggtcc atggcaaaag ccactttcag cttggtgccg
     1081 actccgtcca cgctcgatac gagcacggga tcagcgtatt tggttttgtc tacggcaaac
     1141 agcccgccaa aaccgccgat ttcgctgagg acgccccgag tgaaggtttt gtgggccagg
     1201 tacttgatgc gctgcttggt acggttggcg cggggaatgt ccacgccggc gtcggcgtaa
     1261 gtgatcgtcg gagcgctggc tggcggaatc ttggccacag aatgcttctt tcgtgagttt
     1321 tgcgatccgg agtgaggtca gggccgggcg ccgaagcgac aaccattcct aaggccgctc
     1381 attgtaacac gggagaaagg ggacgctctc gcggggtgag catagcgatt gcagaattca
     1441 gatttgagat tgtagatttg aaaaccaacc cctgcgtctg cgagatagca ctctgaaatc
     1501 tgcaatctga aatgaaacgg ttccgcgtac acggacaacg catccgcagg cgcaaacgcc
     1561 cgttcgccag agcgaacccc aacctagtgg gagatgtact ccgagaaaaa gtacgccaga
     1621 accccgaaca gggccagacc acagatgccg tagcaagtca gcacccaggc tccgcggcct
     1681 tcgtgttgtt tggtggtagc agaggactgc tcggtagaag agacgacagg ggcccgatct
     1741 tccatgcaaa aacctcccga cggaaagatc gtggcgcaag tacgttctgc gtcacgcctg
     1801 ccgccgattt gggcggggca ggactatctg aggcgaattt taccccggtt ttgtgcggtt
     1861 ccgacagaaa aatgtgaccg aaatcacatc cggtttgggg caccgtccgt gggataatag
     1921 ctactaggct tttcaaccac ttatgcggaa ctccagcctg ttttttcttg tctgctgcct
     1981 tctggccgcc ggcgcaaccg cccagcagtc cggcccaacg cctgccgccc aggggtcccg
     2041 ccctgcagcc cagtcgggtt ccgctcagca atccggtggc gcttccgccg cgaagcccag
     2101 cgcttcgaca gccgacaagg cccaggacta ttcgcaggaa tcgttcgtca tcgagcaact
     2161 gcactcgcgc tatcgctttg agtccgacgg caccggccgc aaggaagtgg tcgcgcgcat
     2221 tcgggtgcaa agtgaagccg gcgtgcagca gtggggacag gttcagatcg gctacaactc
     2281 cgagaatgag cgggtcgaga ttccctacgt gcgcgtgctc aaagaagacg gcaatgtggt
     2341 caaggccggc gacgacgcgg tgcaggacct cagcgctcct ttcgagcgcg aagctccggt
     2401 ttataccgac tatcgccaga agcacatcac cgttcccggg ctgcgcccgg gcgaggttct
     2461 cgagtacgac ctggtcaccg tggtgcacac gccgctggct ccggggcaat tctggactga
     2521 ataccagttc gacaagaaca gcattgtcct cgacgaaacg gttgacgtgg aagttccctc
     2581 gagccgcact ctgaaactga aaagcaagcc gggcctcgat cctgcgattc gcgaagagaa
     2641 cggacgccgc atctatcact ggtcgagctc gcatcttgtg cgcgaagacg acaaggacaa
     2701 agacaaagag aagaagaagc accatcccga cgacgatcgg cccgacattc aactgacgac
     2761 ctttgaaagc tgggagcagg tcgggcgctg gtatgccgga ctggaaaaag accgtcgcgt
     2821 cccgagtccc gaggtccgtg ccaaagccgc cgaactgacg aagggcctca cgaccgatct
     2881 cgataaaacg gaaggcctct acgactttgt cgccaagaac ttccggtacg taagcctgtc
     2941 gctgggagtc ggccgctatc aaccgcacgc ttcttccgac gtgctgcaca accagtatgg
     3001 cgattgcaag gacaagcaca cgctgctggc atcgctgctc gaggccgaag gcctgcacgc
     3061 ctcgtcagtg ctgatcaact cgagccgcaa gcttgatccc gacgttcctt caccgtcgca
     3121 gttcgaccac gtcatcacca tgctgccgtt ggggaaaaac gaagtctgga tggacacgac
     3181 cagcgaggtc gcgcccttcc gcctgctcgc tttcaccttg cgcgacaagc aggccctggt
     3241 gattccaccc gccaacccgc cgtctcctgt cccgccgcat ctggaagaaa cgccggccga
     3301 cactcccatg cccgacaccg aactggccga tattgacggc aagatcaacg acattggcaa
     3361 gctcgaagcg cacgtgcact acgagttccg tggagatgag gagcttctgc tgcgttccgt
     3421 gttccgccgc gtgcctcaag ccaactggca gcgggtagtc gagaatgtga acgccagtct
     3481 cggcggagac atcaccaacc tcaaggtcag cgatccggcc gctacgcggg agcctttcac
     3541 gctcacctac gacgttgcca aggtgaactt cctcgactgg tcaaagaaga aaaccgagat
     3601 cggcctgccg ctcgtacagt tcagccttcc cgatgtcgac gacgacaccg acgctgatgc
     3661 cgagccgctg aagctagggc ccaaggccgc gtactcgtat cacatcaaac tggaattgcc
     3721 cgccaaatac accgcgcacg ccccgctggc tttcgcactg aagcgcgact acgcggaata
     3781 cgaagctacc taccatcttg atagtgacgt tctgacagcc gggcgcaagc tcacgcttcg
     3841 tcagtcggag ctgcccgctg cccgagcagc ggactacctg tcattccgcc gcgccgtcga
     3901 atccgatctc gcccagcaag tctcgatgga gagttcggtc gcgggaacgc cggtgcctcc
     3961 tgccgacatg aaggctgacg atctggtgga tagcgcccgc gccgctgccg acaacggcaa
     4021 cctgcccgtc gcgattgccc tgctcaagcg tgccaccgag gtcgatccca aaaacaagtt
     4081 cgcctggaac aacctgggcc tcgcatactt ccagatgcgt gaagacgctc aggccatcgc
     4141 ggccttcggg aagcagattg aagtcaatcc ctacgacgag ttcgcctgga acaacctggg
     4201 ccgcgtgtac tg
//
LOCUS       DASPUL010000677         5013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_813264, whole genome shotgun sequence.
ACCESSION   DASPUL010000677 DASPUL010000000
VERSION     DASPUL010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813264"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1644
                     /gene="asnB"
                     /locus_tag="VEJ00_15555"
     CDS             <1..1644
                     /gene="asnB"
                     /locus_tag="VEJ00_15555"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXX02632.1"
                     /translation="DTEVIVHLYEEMGADCVSKLRGMFAFALYDERRRKLLLARDRLG
                     KKPLHYALDGGRLWFGSEIKSILTVAPELADIDREALLQYMYFGYIPDPLTAFTKIQK
                     LPPGHLLEFEDGKISVRQYWDLPRYGTHPPRSEEECLEEMEQRLAEAVRIRLISDVPL
                     GALLSGGTDSSTVVALMARATSQPVKTFAIGFRDQEFNEAPYARKVAEKFGTDHHELI
                     VEPDVVETVETLTSSLEEPFGDSSMLPTYYVSCLARKYVTVALSGDGGDEIFAGYDRY
                     GIHLRRKVFERIPGWARRIYREQVYPRLPGDMRGKKFSYNVSLPWRERYVDGISFVPA
                     FERDMPLLSEDFRSVLNNGGNPERVMYRYFERAPAGDPVSQMLYVDTKTYMVADILTK
                     VDRMSMATSLEVRVPILDHVFVEWATGLPAEWKIRRGQQKYILRKLAERVGVPREVLY
                     RSKQGFTLPLVHWIRHELKEMIQSVLLDARTLQRGYFDPRGVRQLLDEHFRGRRNHYG
                     RIWRLLMFELWHRNYLERVRASNANDEPCPVTRTAGTLG"
     gene            1641..3956
                     /locus_tag="VEJ00_15560"
     CDS             1641..3956
                     /locus_tag="VEJ00_15560"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXX02633.1"
                     /translation="MSPLTDQQTQAILNTADGAQGPPAAVFSEPVFLMINSLETGGTE
                     RQFVEMARALRADRIPVHLGCVRNRGALAEGLGDLPEFRLGGSVYGWRSLRSRWRLKR
                     HLAGLKVAVAHAFDFYANLTLIPAARLAGVPVVIGSHRQLGDLLTPAQFRAQLAVFRM
                     CDRVVCNSRAAAERLLWAGLPERKVVVIGNALPGELFEEHVPALPPAEGMLRIGMIAR
                     MNAEYKNHRGFLRAAKQLSQRFPNAEFVLAGDGPLRAQLEQEAAELGLKGQVRFLGDR
                     RDVAAVLGSLDISVVPSSSESLSNAMLESMAAGVSAVATAVGGNCEIGSDGRAVLVPV
                     NDDQALAAGLARLVQDVRLRADMACRARDFVQANFSEDHICRQYEELYAETLQEQRSH
                     SRRGSGLRPRADSAIRVALVAPSLRYVGGQSVQGDLLLRHWAHDPEVAARFVAVDPRL
                     PFGLGWAEKVPFLRTALRAPLYLWRLWRDLKDVDVAHIFSASYTSFLVAPLPAWLVAK
                     ARGKKTMINYRSGEARDHLQHSWIARRVLRSSDRLVVPSGYLVEVFREFGLEALIVPN
                     MVDLSQFQYRERRPLRPHLACTRGFHPYYGIDVVVRAFAEVQKAYPDAQLDLVGGGRL
                     EGSIRELVRQLNLPNVNFAGVVSRREIGRAYDRADIFVNASNLDNMPVSVLEAFASGT
                     PVVTTEPEGMRYVVEHERTGLLSAPGDATALAGNVLRLLQGPDLATRLAENALAETRR
                     YSWAALREQWLGAYAQLVRRSDKTIETVPAD"
     gene            3999..>5013
                     /locus_tag="VEJ00_15565"
     CDS             3999..>5013
                     /locus_tag="VEJ00_15565"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXX02634.1"
                     /translation="MISQTHSRRDLESATETSGRKRAPVFVVGCPRSGTTVLYHMLLS
                     AGGFAVYRSESNVFNLLAPRFGGMRSRRHREELMDTWLRSILFRVSGLDAAHIRARVF
                     EECRSGGDFLRIVMGEVAENQGVERWADCTPEHLLSMKEIKRQIPDALFIHIIRDGRD
                     VALSYLKQGWSHPLPWDQQKELEVTGLYWNWIVGQGREAGKALGADYQEVRFEDLTAR
                     PQEALVRLGEFIDHDLDYETIRKAGIGSVSAPNSSFDDEDGQFHPVGRWKTRMSQEQL
                     REFEALAGDRLEELGYPLASGKPAASLRAARLRATYFAMFRAKQWMKTHTPLGRRVRL
                     GRIE"
BASE COUNT          882 a         1319 c         1822 g          990 t
ORIGIN      
        1 gatactgagg taattgtcca cctctacgag gagatgggcg cggattgcgt gagcaagctg
       61 cgcgggatgt ttgcctttgc gctctatgac gagcgacggc gcaagctgct gctggcccgc
      121 gaccggctgg gcaagaagcc actgcactat gcgttggacg gcgggcggct gtggtttgga
      181 tcagagatca aatcgattct gaccgtggct ccggagctgg cagatatcga tcgggaagcg
      241 ctgctgcagt acatgtattt cggctacatt cccgatccgt tgacggcgtt tacgaagatt
      301 cagaagctgc cgccggggca tctgctcgaa ttcgaggacg gcaagatcag cgttcggcaa
      361 tattgggatc tgccgcggta tgggacgcac ccgccgcgca gcgaagaaga gtgcctggaa
      421 gaaatggagc agcggctggc ggaagcggtg cgtatccggc tgatctctga tgtgccgttg
      481 ggcgcgctgc tgagcggcgg gacggattcc tcgaccgtgg tcgcgttgat ggcgcgggca
      541 acctcgcagc cggttaagac ctttgccatc gggtttcgcg accaggaatt caacgaggcg
      601 ccgtatgcgc gaaaggtcgc ggaaaaattc ggcaccgatc accatgaact catcgtcgaa
      661 ccggatgtcg tggagacggt cgaaacgctc accagttcgc tggaggagcc gttcggcgat
      721 tcgtcgatgc tgccgacgta ttacgtctcg tgcctggcgc gaaagtatgt gaccgtagcg
      781 ctgtcgggcg acggcggaga cgagattttc gccggatacg accggtatgg cattcatctt
      841 aggcggaagg tattcgagcg aattccgggc tgggcgcggc ggatatatcg cgagcaggtt
      901 tatccgcggc tgccggggga catgcggggg aagaagttct cctacaacgt ttcgctgcct
      961 tggcgggagc gatatgtgga cgggatctcg tttgtcccgg catttgaacg cgacatgccg
     1021 ctgctgtcgg aagactttcg gtccgtgctg aataacggcg ggaatccgga gcgcgtgatg
     1081 taccggtatt tcgagcgggc tccggccggt gatccggtca gccagatgct ttacgtcgac
     1141 accaagactt acatggtggc cgacattctg accaaagtgg accggatgag catggcgact
     1201 tcgctcgaag tgcgcgtgcc gattctcgat cacgtctttg tggaatgggc gacgggattg
     1261 ccggcggagt ggaagatccg gcgggggcag cagaagtaca ttctgcgcaa actcgcggag
     1321 cgcgtcggcg ttccgcgcga ggttctgtac cggtccaagc aagggttcac gctgccgctg
     1381 gtgcattgga tccggcacga gctgaaggaa atgatccaga gcgtgctgct cgatgcgagg
     1441 acgctgcagc ggggatactt cgatccgcgc ggcgtgcggc agttgctgga cgagcatttc
     1501 cgcggacggc gcaatcacta cggacgaatc tggcgactgc tcatgttcga gctgtggcat
     1561 cggaactacc tggaacgagt ccgtgcgagc aatgcaaacg acgagccgtg ccccgtgacc
     1621 cggacggctg gaaccctggg atgagtccgc tcaccgatca gcagacgcag gcaatcctga
     1681 acaccgcgga tggcgcgcaa ggtcctccgg ctgcggtttt ctccgaacct gttttcctga
     1741 tgattaacag cctggagacg ggcggcacgg agcggcagtt cgtcgagatg gcgcgcgcgc
     1801 tgcgggcgga tcggattcct gtacatctgg ggtgcgtgcg caaccgcggt gctctggcag
     1861 agggcttggg ggatttgccg gagttccggc tcggcggcag cgtgtacggc tggcgatcgc
     1921 tgcgcagccg ctggcggctg aagcggcatc tggcggggct taaggtagcg gtggcgcacg
     1981 cgttcgactt ctacgcgaac ctcacgctga ttccggcggc gcggctggcg ggagttccgg
     2041 tggtgatcgg tagtcaccgt cagctcgggg atttgctgac tccggcgcag ttccgggcgc
     2101 agcttgccgt gttccgcatg tgtgaccgtg tagtgtgcaa ttcgcgcgcg gcggcggagc
     2161 ggttgttgtg ggccggattg ccggagcgga aagtggttgt gatcggtaat gccttgcctg
     2221 gtgagctgtt cgaagagcat gttccagcgc ttccgcccgc cgagggcatg cttcgcatcg
     2281 gcatgatcgc gcggatgaac gcggagtata agaaccatcg cgggtttctg cgggctgcca
     2341 aacagttgag ccagaggttt ccgaacgcgg agttcgtgct ggcgggcgat ggtccgctgc
     2401 gggctcagct ggaacaggaa gccgcggagc tgggattgaa agggcaggtg cgattcctgg
     2461 gagaccggcg cgatgtggcg gcggtgctgg ggtcgctcga catttccgtg gtgccttcga
     2521 gctccgagag cctgtcgaac gcgatgctgg aatcgatggc ggcaggtgtg tcggcggtgg
     2581 cgacagcggt gggcgggaat tgcgagatcg gcagcgacgg acgggcggtg ctggttccgg
     2641 tgaatgatga tcaggcgttg gctgcggggt tggctcggtt ggtgcaggac gtccgcctgc
     2701 gggccgacat ggcgtgccgc gcaagggatt tcgtgcaggc gaatttcagt gaagaccaca
     2761 tctgccggca gtacgaagag ctctacgccg agacgctgca ggaacagcgc tcacattcgc
     2821 ggcgtggttc cggtttgcgg ccgcgcgctg attcggcgat ccgggtcgcg ctggtggcgc
     2881 cctcgctgcg ctacgtcggc ggccagtccg tgcaagggga cttgctgctg cgacactggg
     2941 cgcacgatcc ggaagtggcg gcgcgcttcg tagcggttga tccacgactt ccttttggat
     3001 tgggctgggc cgagaaagtt ccgttccttc gcactgcgtt gcgggcgccg ctctacctct
     3061 ggcggctatg gcgagatctg aaggatgtgg acgtggcgca cattttttcg gcgtcctaca
     3121 cgtcgttttt ggtggcgccg ctgccggcct ggctggtggc gaaggcgcgg gggaaaaaga
     3181 cgatgatcaa ctacaggagc ggcgaggcgc gggatcactt gcagcattcc tggatcgcgc
     3241 ggcgcgtgct gcgaagttcg gatcggctgg tggttccatc gggatatctg gtggaggtct
     3301 tccgcgagtt cggtctggag gcgctgattg tgcccaacat ggtggacctg tcgcagtttc
     3361 agtatcggga gcgacggccg ttgcgaccgc atctggcgtg cacgcgcggg tttcatcctt
     3421 actacggaat cgatgtcgtg gtaagggcgt ttgcggaggt gcagaaggcc tatcccgacg
     3481 cgcagcttga tctggttggt ggaggaaggc tggaaggaag catccgggag ttggtgcggc
     3541 aactgaatct gccgaacgtg aactttgcgg gtgtggtatc gcggcgagag atcggacggg
     3601 cgtacgatcg cgccgacatt tttgtaaatg catcgaatct cgacaacatg ccagtttcgg
     3661 tgcttgaggc ttttgcctcg ggtacgcccg tcgtgaccac ggagccggaa ggaatgaggt
     3721 atgtggtgga gcatgagcgg accgggttgc tgtccgcgcc cggcgatgcg acggcgctgg
     3781 cgggcaacgt cctcaggctc ttgcaaggcc ctgacctggc gacgcggttg gcggaaaacg
     3841 ctttggcgga gacgcgccgt tacagctggg ctgcgcttcg agagcagtgg ctgggagcgt
     3901 atgcgcaact tgtgcggcgc agcgacaaga caatagagac tgtgcccgcg gactgatcaa
     3961 cgccggacaa cccgcgacgg ggcgagtcaa ttctagcgat gatttcccaa acccacagcc
     4021 gacgcgacct tgagagcgcg accgaaacca gcggccgcaa gcgggcgccg gtgttcgtgg
     4081 tgggctgtcc gcggtcgggg accacggttc tctatcacat gctgctctcg gcgggaggct
     4141 tcgccgtcta ccggtcggaa tcgaatgtct tcaacctgct ggcgccacgg tttggaggaa
     4201 tgcgctcgcg ccgccatcgc gaagaactca tggacacctg gctgcgcagc atcctgtttc
     4261 gcgtgtcggg gctggacgcg gcgcacatcc gggcgcgcgt gttcgaggag tgccgcagcg
     4321 gcggagactt cctgcgcatc gtgatgggag aagttgcgga gaatcaggga gtggagcgct
     4381 gggctgactg cacgccagaa catctgctct ccatgaaaga gatcaagcgg cagattcctg
     4441 acgcgttgtt cattcacatc attcgcgacg ggcgcgacgt tgcgctgtcg tacctgaagc
     4501 agggatggtc gcatccgctg ccgtgggatc agcagaaaga gctggaagtc accggtttgt
     4561 actggaactg gattgtgggc caggggcgcg aggcgggcaa agctctggga gcggattacc
     4621 aggaggtgcg gttcgaggat ctcacggctc gtccgcagga agcgctggtg cgtctgggag
     4681 agttcattga tcacgatctg gattatgaga cgattcgcaa agcgggaatc gggtcggtga
     4741 gcgcgcccaa ttcgtcgttt gacgatgagg acgggcagtt tcacccggtc ggacgctgga
     4801 agacgaggat gtcgcaggag cagctgaggg agtttgaggc gctggccggc gaccggctgg
     4861 aggaacttgg gtatccgctg gcgtcgggca aaccagcggc gagcttgcgc gctgcgcggc
     4921 tgcgggcaac gtactttgcc atgtttcgcg ccaagcagtg gatgaagacg catacgccgc
     4981 tgggacggag ggtgcggttg ggaaggattg aga
//
LOCUS       DASPUL010000678         3166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1780203, whole genome shotgun sequence.
ACCESSION   DASPUL010000678 DASPUL010000000
VERSION     DASPUL010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780203"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1521
                     /locus_tag="VEJ00_15570"
     CDS             187..1521
                     /locus_tag="VEJ00_15570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HXX02635.1"
                     /translation="MGFPRPKYCLQPAFAALSAVLLLAVPSYAGKQLSGAIPGEIAAA
                     DPSGAAHFNCELRPFDLSKGLYCYGPDAIRAAYGVDALLSNGFNGAGQTIVIIDAFGS
                     PFALADLQRFDAVFGLPDPSFTQIYMPGTPPFDPNNPNIVNWTAEIALDTQWSHAIAP
                     KAAIVLVAAKSNDDVDLIAALNYALDNHLGNVVSMSFGESEVFLANPDGLDIVQAWTD
                     AFTKAKKQRVTLFVSSADDGVDTGLIGTPNVSFPASSPLVTGVGGTNLQFGTATNADP
                     NGTYQAEEVWNDPFGAGGGGMSILFAEPDFQSNNVPRSITKTLHHYRGVPDVAYNAGV
                     VGGVIAFWAYPAAGDAYIFGGTSAGAPQWAGIIADLNQAKGRPLGYINKQLYSLGGCG
                     GLAKLFHDIVTGDNGIPGVPGYPALPGYDLSTGWGTPNFGKLGMVLNDPDDN"
     gene            1807..2124
                     /locus_tag="VEJ00_15575"
     CDS             1807..2124
                     /locus_tag="VEJ00_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02636.1"
                     /translation="MRLKTLVATVFLGTLMGCGGSGVGPRSGNGALTESDGGAPGEDG
                     GTDAGIVSGMDGGTSSADGGTEACDCTGLALPDVCMVCSDGMSACAHFVCAQGTCEVQ
                     ICQ"
     gene            2143..2661
                     /locus_tag="VEJ00_15580"
     CDS             2143..2661
                     /locus_tag="VEJ00_15580"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXX02637.1"
                     /translation="MAFKRSPVRSRFAPLIRTRLPLAFAALAVASACSRPSFDDAAEA
                     QVLLKRDREWAALAAAGKDPDAVAAFWTADALVYPPGRPILEGRAAIRAYVAESFKIQ
                     GFRIHWVSEKPSFAADGKMAYMRSTNEITVPDSQGTPMTISARGVTVWRLEPDGHWRC
                     AVDIWNDGPKLK"
     gene            2978..>3166
                     /locus_tag="VEJ00_15585"
     CDS             2978..>3166
                     /locus_tag="VEJ00_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02638.1"
                     /translation="MSPRVCAVALVLFLSCLEGARASTGPDASSAEAAVASPRSELGP
                     ERYVGTYLYAGTDAERATI"
BASE COUNT          537 a         1046 c         1030 g          553 t
ORIGIN      
        1 gcccgtttgg gtaggggtcg attcttaccc atgccgccct tggctgcgcc ctcaggcagc
       61 gaggagcgcc gaaaggccaa tgaagcaacc agccttgcgg gcgactgcac gctgtttgta
      121 ctgtgcgccc gccgaccacc acgtcgtcca tccacccacc tgcaagcaga ttcaaaggag
      181 cgccgcatgg gctttccgcg tccgaagtac tgcctgcaac cagcgttcgc cgccctgagc
      241 gctgtgctgc ttctcgccgt tccctcctac gccggcaagc agttgtcggg agcgattccc
      301 ggtgaaatcg ccgccgcaga tccgagcggg gccgcacatt tcaattgcga gttgcgtccg
      361 ttcgatctga gcaaaggcct ctactgttat gggccggatg ccatccgcgc cgcctacggc
      421 gtcgatgcac tgctcagcaa cggcttcaac ggtgccgggc aaaccatcgt catcatcgac
      481 gcctttggca gccccttcgc cttggccgac ctgcagcggt tcgacgcagt gttcggcctt
      541 cccgatccca gcttcaccca gatctacatg ccggggacgc ctccgttcga tcccaacaac
      601 cccaacatcg tcaattggac ggccgagatc gccctcgaca cccagtggtc gcacgccatc
      661 gcgccgaaag cggccatcgt cttggttgcg gcaaagagca acgacgacgt ggacctcatc
      721 gccgccctga actacgccct cgacaatcat ctcggtaacg tcgtctcgat gagctttgga
      781 gagagcgaag tcttcctcgc caatccggac ggcctcgaca tcgtccaggc ctggaccgac
      841 gccttcacga aggccaagaa gcagcgcgtg acgctcttcg tctcctccgc cgacgacgga
      901 gtcgataccg ggctcattgg cacgccgaat gtgtccttcc ccgcctcgtc gccactggtc
      961 actggcgttg gtggaaccaa cctccagttc ggtacggcca ccaatgccga cccgaatggg
     1021 acgtaccaag ccgaggaggt gtggaatgat cccttcggcg cgggaggggg cggaatgagc
     1081 atcctgtttg ccgaacccga tttccagagc aacaacgtgc cgcgctcgat caccaagacg
     1141 ctgcaccact accgcggtgt gcccgacgtg gcctacaacg ccggcgtcgt gggcggcgtc
     1201 atcgccttct gggcgtaccc agccgccggc gatgcctaca tcttcggtgg caccagtgcc
     1261 ggggcaccgc agtgggccgg aatcatcgct gacctcaacc aggccaaggg ccggcctctg
     1321 ggctacatca acaagcagct ctacagcctg ggaggttgcg gagggctcgc caagctcttc
     1381 cacgacatcg tcaccggcga caacgggatt cccggcgtcc ccggatatcc agcgctgccc
     1441 ggctatgacc tctcgaccgg ctggggaacg cccaacttcg gcaagctggg catggtcctc
     1501 aacgatccgg acgacaacta gagctcggcc gtcgttcaca acggctgtgc gaacggcctc
     1561 cggcgcggag ccggaggccg tttcattttt ccgggtgacg tcctctccgc gaaaaggcgc
     1621 cgcctgtcgc cgctgcatct cgagcttctc tggggagatc gaaaagctac agcaccacca
     1681 ccagcctgtc ctgcgacctg gggttgcgcg ccggttcgag gtccctggtg atagggtgac
     1741 tcgcaacgtc tcgacgggat ggacgtgcat gcttcacagg cgatgccccg gagaggaggg
     1801 cccaggatga gattgaaaac actcgtggcg acggtcttcc tgggcacgct gatggggtgc
     1861 ggcgggagcg gagttggtcc tcggtccggg aacggcgctt tgacagagtc cgatggtggc
     1921 gctccgggtg aggatggcgg aacggacgcc ggcatcgtga gtggcatgga tggtgggacg
     1981 tcatccgcgg atgggggcac cgaggcgtgc gactgcaccg ggttggccct gccagatgtc
     2041 tgcatggtct gcagcgacgg catgagcgcc tgcgcccact tcgtctgcgc tcagggaacg
     2101 tgcgaagttc agatctgcca gtagggcggg ccgcttcttc tcatggcatt caagaggtca
     2161 ccggttcgat cccggttcgc tcccctgatc cgtacccggc tcccgctcgc gttcgccgcg
     2221 ctggccgtgg cttcagcctg ctcgcgtccg tcgttcgacg acgccgccga ggctcaggtg
     2281 ctgctgaagc gtgatcggga gtgggccgcg ctcgcggccg caggcaagga tccggatgcc
     2341 gtcgcggcat tctggacagc ggacgccctg gtgtacccgc ccgggcggcc gatcctggag
     2401 gggcgcgcgg ccatccgcgc ctatgtggcc gagagcttca agatccaggg cttccggatc
     2461 cactgggtct cggagaagcc gagcttcgcg gcggacggga agatggccta catgcgcagc
     2521 accaacgaga tcaccgtgcc cgactcccag ggaacaccga tgaccatctc tgcgcgaggc
     2581 gtcaccgttt ggaggctcga gccagacggc cactggcggt gtgccgtgga catctggaac
     2641 gacgggccaa agctgaaata gggatgaagc cggtcaacgg cgccgggagg ggaggccggc
     2701 gcgggcgccc gcacgagagg gccggacatg ggttcgaaga gcccgctcgc gccgcaggca
     2761 ccggctttgc acgggcacgg tgctgagagc gtcgatgctg gcttgtttgc cgctttcaca
     2821 ggtgaggtgg attcgatgtt gcgggtgatc acgagaggga gggggacgcg ctgggccgcc
     2881 tgctaccgcc caccgggcat tcctccggga gtcacccgat ctcggacgaa gacacgggtc
     2941 gacgctcctc ctggctaacc gtgggcggcg agtcgtcatg agcccgaggg tttgcgcggt
     3001 cgctctcgtc ctgttcctct cttgtctcga gggcgcccgc gcctcaacgg gtcctgatgc
     3061 ctccagtgcc gaggcggccg tcgccagccc acgctccgag ctcgggcccg agcgttatgt
     3121 cggaacgtac ctctacgcgg gaaccgatgc ggaaagggcg acgatc
//
LOCUS       DASPUL010000679         3377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1780240, whole genome shotgun sequence.
ACCESSION   DASPUL010000679 DASPUL010000000
VERSION     DASPUL010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780240"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2755
                     /locus_tag="VEJ00_15590"
     CDS             <1..2755
                     /locus_tag="VEJ00_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02639.1"
                     /translation="RHTLFVRGAGQDLRNPGAPFLPGDIPEQTTLDHSKGFVLGYIAV
                     LSPSLTNSFHWGFTRQSFGIIGNTDAQWNTFLGLDQGISFSHNFQVPLHNFVDDVSWT
                     KGTHTFQFGAAIGIARDPRTSYLHSNTLGLGTTNWTSPIGFAGTTSTLDPLNAGAHPT
                     LSVPNFPVGTAPEPQTATQYDRPLLALYGMISDVVANYNLDRSGNPQAQGTPVKRNYG
                     LDWYEFYGQDTWRLKPNLTVTYGLRWSLFPAPWETNGYQTASTFGLGTQFAKNVKNME
                     QGIGYGGEPPIEFALSGPVNHGPGFYPLEKSDVSPRISIAYSPRFSDGPLRKLFGDND
                     KTVFRLGLSRVYDRAGFTLLNSFDQIGAAGLSTTIQNTCCTAGFTSAEDLPRISGIHN
                     IPTVNLNNAPFLLPPPAPGFPQTPTVSAQANLWGVDNTMKTPHAYTVDFSVGRELPKR
                     FSLQLSYVGRLGRNLLTQRDLTQPLDIVDPKTGIDYYTAASALSNLARSFALANNGGT
                     PTNYYQGLPTTAQISSVTASMLGKTAQYWVDMLPQLRSGATQYRDWLDCFDPTATNPT
                     DGLVQTVFDLYYNPALSVIGDEIVGLADVDLYGGLIDNTIPCSTTTPNSYFFNGPPGL
                     LGNNSGQFLNNQAISMYGWSSIGSSSYHALQANLRKQLSRGVQFDLNYTYSKSLDVTS
                     AASRVGFAVYGYTNIGLVGSRLANAFSPNLARAPSDFDLTHQINLDWVADLPVGKGRA
                     LAGNAGGVLNAIVGNWQLSGLARWTSGFPFSVDGGQRWPTDWFLTAITQMTAKPKTGT
                     FKGNGSVNIFGDPTAAAAAQQDFTLPLPGQVGSRNVLRGNGYAGWDMSLVKRWRMPYN
                     DSNTLQFRWEVFNVPNLTRFNAQGVGSSLLTSLTQQPASFGAYTSLLTQPRVMQFALR
                     YEF"
     gene            2983..>3377
                     /locus_tag="VEJ00_15595"
     CDS             2983..>3377
                     /locus_tag="VEJ00_15595"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXX02640.1"
                     /translation="MDWKEHGIKIVRAKELDSNTPQTPGMTRAAAITHARTGASKLWA
                     GTVVVQPHARTGPHHHGELETVIYVVRGRARMRWGDHLEFCEEADPGDFIYVPPYVPH
                     QEINARPDMPCEAVVVRSGQDPIVVNLDI"
BASE COUNT          677 a         1089 c          960 g          651 t
ORIGIN      
        1 ccggcacacc ctgttcgtgc gtggggcggg tcaggacttg cgcaaccccg gtgccccgtt
       61 cctgccaggt gatattccgg agcagacgac gctggatcac agcaaaggct tcgtcctcgg
      121 ctacatcgcg gtgctgagtc cttctctgac caactccttt cattggggat tcacgcggca
      181 gagcttcggg atcatcggca ataccgatgc gcaatggaac acttttctgg gcctggatca
      241 gggcatcagc ttcagccata acttccaggt gccgctgcat aacttcgtgg atgatgtgtc
      301 gtggaccaag ggcacgcaca cgttccagtt tggggcggca atcgggatcg cgcgcgatcc
      361 ccgcaccagc tacctgcact cgaacacgct gggccttggc acgaccaact ggacttcccc
      421 catcggcttc gcgggcacca ccagcacgct cgacccgctg aatgcaggcg cacatccgac
      481 gctctcggtt ccgaactttc cggttggaac ggcccccgag ccccagactg caacccagta
      541 cgaccgaccg ctgctggcgc tgtacggcat gatctccgac gtcgtggcca actacaacct
      601 tgaccgcagc ggcaacccgc aggcccaagg cacacccgtc aaacgaaact acggcctgga
      661 ttggtacgag ttctacgggc aggatacgtg gcgcctcaaa ccaaatctca ctgtgactta
      721 cggattgcgc tggtcgcttt ttcccgcgcc ctgggagacc aacggatacc aaaccgcttc
      781 gacgttcggt cttggaacac aattcgcgaa gaatgtgaag aacatggagc aaggcatcgg
      841 ctacggaggc gagccgccca tcgagttcgc cctgagcggg ccggtgaatc acggtcccgg
      901 gttctatccc ctggaaaaga gcgacgtgtc gcctcgcatc tcgatcgcct actcgccacg
      961 cttcagcgac ggccctctta ggaaactgtt cggcgacaac gacaagaccg tttttcgcct
     1021 ggggctcagc cgggtgtatg accgggcggg atttacgctg ctgaacagct tcgatcagat
     1081 cggcgccgct ggcttgtcca ccaccatcca gaacacctgc tgcacggcgg gtttcacgag
     1141 cgccgaggat ctgccccgga ttagcggcat ccataacatt ccgactgtca atctgaacaa
     1201 cgccccgttc cttctgcccc cacccgcgcc gggctttccg caaacaccga ccgtcagcgc
     1261 ccaagccaat ctgtggggcg tcgacaatac gatgaagacg cctcatgcgt acacggtgga
     1321 cttctccgtt ggacgggagc tgcccaaacg gttctcgctc cagctctcgt acgtaggccg
     1381 tctcgggcgc aatttgctta cgcaacgcga cctgactcaa ccgctcgata tcgtggaccc
     1441 gaagacgggg attgactact acacggcggc gtctgcgcta tccaatctcg ccaggtcctt
     1501 cgccctggcc aacaacggcg gcactcccac gaactattac caggggctac ccacaactgc
     1561 ccagatcagc agcgtaaccg cgtccatgct gggtaagacc gcacaatact gggtagacat
     1621 gttgccgcaa ctccgttccg gtgccacaca gtaccgggac tggctggact gtttcgatcc
     1681 aaccgccacc aatccaacgg atggactcgt gcaaacggtc ttcgaccttt actacaaccc
     1741 ggcgctctcg gtaattggcg atgagattgt cgggttggcc gacgtcgact tatatggcgg
     1801 gctcatcgac aatacgatcc cgtgcagtac aaccactccc aactcctatt tcttcaacgg
     1861 cccgcccgga ctgctgggca acaattccgg ccaattcctg aataaccagg caatctccat
     1921 gtatggatgg agctcgattg gcagctcgag ctaccatgcc ctgcaggcga acctgcgcaa
     1981 gcagttgagc cgcggggttc agttcgacct gaactacacc tactccaagt cgctcgacgt
     2041 aacttccgcg gcatcgcgcg tggggtttgc ggtgtacggt tacaccaaca tcggcctggt
     2101 cggcagccgt ctggccaatg ccttttctcc aaacctggct cgcgcccctt ccgacttcga
     2161 tctcacccac cagatcaacc tcgattgggt ggccgatctg ccggtgggca aggggcgggc
     2221 gctggcgggc aatgccgggg gtgtgctcaa cgccatcgtt ggcaactggc agctttccgg
     2281 cctcgcccgc tggaccagcg gtttcccgtt cagtgtggac gggggccagc gctggcccac
     2341 cgactggttc ctcactgcca ttacgcagat gaccgcaaaa ccgaagactg gcacgttcaa
     2401 aggcaacggc agcgtcaata ttttcggcga tccaaccgcg gccgcggcgg cacaacagga
     2461 tttcaccctg ccgcttcccg ggcaggtcgg ctcgcggaat gttctgcgcg gcaacggcta
     2521 tgccggctgg gacatgagcc tggtcaagcg ttggagaatg ccttacaacg acagcaacac
     2581 cctgcaattt cgctgggagg tgttcaacgt gccgaacctg acacgcttca atgcccaggg
     2641 cgtggggtcg tcgctgttga catccctgac gcagcagccg gccagcttcg gcgcttacac
     2701 cagcctgctg acacagccgc gcgtcatgca gttcgcgctg cggtatgaat tctgatatcg
     2761 cgccggttta gacggggggg ttggctcgac ggctggaacc tgcgcgcgtg cccggaaggg
     2821 cgcggtggat ttctgagccg cctgaccgtc ggggaacgtt tgttttgatg aatttcagcc
     2881 ctcaggaaaa cctctcgacg ttctagctct tcacacactt gcatccccac gccgcagaag
     2941 atactctgct gcacgaaatg cttcctgcgg agtgggctat ccatggactg gaaggaacac
     3001 ggaataaaga tcgtacgtgc gaaagaactg gactcgaata cgcctcagac tcccggaatg
     3061 acccgggccg ctgccattac ccacgcgcgc accggggcga gtaaattatg ggctggtacg
     3121 gtagtcgttc agccgcacgc caggacgggc ccgcatcatc acggcgaact ggagacggtc
     3181 atctacgtgg tccgaggtcg cgcgcgcatg cgctggggcg atcatctgga attctgcgaa
     3241 gaggccgatc ccggagattt catctacgtt ccgccctacg tcccgcatca ggaaatcaac
     3301 gcccggcccg atatgccgtg cgaggccgtg gtagtccgaa gcggacagga tccgatagtc
     3361 gtgaacctgg acatcga
//
LOCUS       DASPUL010000680         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1502814, whole genome shotgun sequence.
ACCESSION   DASPUL010000680 DASPUL010000000
VERSION     DASPUL010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502814"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VEJ00_15600"
     CDS             complement(<1..1028)
                     /locus_tag="VEJ00_15600"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXX02641.1"
                     /translation="MTFQHLLDGAEFLSQSGNAEVTGVEYDSRRVKPGNLFVAMHGES
                     SDGNRFIDQAIQAGAVAVVTDSAAEKPREGVAWAVVPSGRRALARVSANFYRRPAERI
                     AISGITGTNGKSTTAFLVESILAAAGRKSALIGTIEYHVAGRVYPAPHTTPEALELAR
                     LFNEAVGQGASDAVMEVSSHALAQERVYGVPFDVAVFTNLTRDHLDYHKTMEEYFAAK
                     QVLFSGCGTDAPRVAVTNLDDEYGAQLVEFSRKRSSVVLTYGWQRGDFHAENVDIAPR
                     GTRFDIITPEEKIAVFSPLIGRVNVYNILAAAAACYARGCDARAIARGVASLTHVPGR
                     FERVDCGQP"
     gene            1535..>2977
                     /gene="pckA"
                     /locus_tag="VEJ00_15605"
     CDS             1535..>2977
                     /gene="pckA"
                     /locus_tag="VEJ00_15605"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HXX02642.1"
                     /translation="MATTVHVSDVAELLSEGRTHFNLSPASLVEHSIRRHEAKLAANG
                     SIVGYTNRTGRSPKDKFIVKDATTEHTVAWGAVNAAIDPEKFDALYARVMEHLRGREL
                     FVQDLFCGADPVYRLPVRIVNEYAWHNLFVHQLFLRPTAAELKTHHPEFTVISAPEFK
                     ADPKRDGVNSEVFILLNFTRRTVLIGGTSYAGEMKKSIFSVMNYILPQRNVFPMHCSA
                     NVGHDGVTALFFGLSGTGKTTLSADPSRDLIGDDEHGWSADGVFNFEGGCYAKCIKLS
                     KKNEPQIWNALRFGCVLENVTLDTHTRVPDYNDDSKTENTRAAYPVDFIDNAVIPGVG
                     GHPKNVVFLTADAFGVLPPISRLTPEQAMYHFFSGYTAKVAGTEAGVKEPSATFSTCF
                     GSPFLPLRPKVYAEMLGRRLREHDAQCWLVNSGWQGGPYGVGKRMEIPFTRAMVDAAV
                     EGELTKEEFEIEPTFGFAIPKACPGVPPQVL"
BASE COUNT          573 a         1000 c          838 g          566 t
ORIGIN      
        1 ggctgcccgc aatcgacccg ctcaaacctt cccggcacgt gtgtcaggga cgcaacgccg
       61 cgggcgattg cgcgtgcgtc gcagccccgc gcatagcagg cagcggccgc agcaagaatg
      121 ttgtagacat tcacccgccc gatcagcggc gaaaagacgg caatcttttc ttccggcgtg
      181 attatgtcga agcgcgtccc gcgcggcgcg atgtcgacgt tctccgcgtg aaaatcaccg
      241 cgctgccatc cgtaggtcag gacgactgaa cttcgcttgc gactgaattc cacgagctgc
      301 gcgccgtatt cgtcgtccag gttggtcaca gcgactcgcg gtgcatcggt cccgcagccg
      361 ctgaacaaca cctgcttggc ggcgaaatac tcctccatcg ttttgtgata gtcgaggtga
      421 tcgcgcgtca ggttggtgaa cacggccacg tcaaacggca cgccgtacac gcgctcctgc
      481 gccagcgcat gcgacgaaac ttccataacc gcgtcgctcg ccccttgccc gacagcttca
      541 ttgaacaggc gcgcgagttc gagcgcctcc ggcgtcgtat ggggcgcggg atagacgcgt
      601 cccgcaacgt gatactcgat cgtgccgatc agggcgctct tgcgtccggc cgcggcaaga
      661 atggactcca ccagaaacgc tgtcgtgctc ttcccgttcg tccccgtgat tccactgatc
      721 gcgatccgct ccgccggccg cctgtagaaa ttggcgctca cccgcgccag cgcccgccgc
      781 ccactgggaa ccaccgccca cgccacaccc tcgcgcggct tctcggcagc agaatcggtc
      841 accactgcga ctgctccggc ctgaattgcc tgatcgatga agcgattccc gtcagaactt
      901 tccccgtgca tggccacgaa caagttgccg ggcttgaccc gccgagagtc gtattccaca
      961 cccgtcactt cggcgttccc gctctgcgaa aggaactccg ccccatccag cagatgttgg
     1021 aaggtcattg ctggagatta taaagtcagg ggccagggat cagaggttag gggtcagagg
     1081 tcaggaaaca tttagtgctc gccctgttcc ctgacccctg gcccctgagc cctgcccatt
     1141 acctccgtca cgttccgccc ccccgccggc gcgcgctaca gtagccgcat gagctccgga
     1201 accactcata tcaagtatcg ctgtatgcaa tgcgagcagc tggaagacat ctgccagtgc
     1261 gaaaagtact gcgttctttg ccaggcgatc atcgacattc gcatttgcca ggacggactc
     1321 atgtactgcg agccctgccg caaagcctgc gactacaaaa cctcttctga ttagcgcgac
     1381 cctcgccgtg ccggcaacct ccttgttatc ctgaaggtag acacgtccgc agcgcatcga
     1441 cgtgatcccg gtccttggca agccaaggag ctttaaggca gaatagggcg tttttgcccg
     1501 gttccgtttc gggcgccctc atacaggaga attcatggct accacggtgc acgtcagcga
     1561 cgttgccgag ttgctgagcg aaggccgaac ccacttcaac ctttcaccgg cgtcgctggt
     1621 cgagcactcc atccgccgcc atgaggctaa gctcgctgcc aacggctcaa tcgtgggcta
     1681 caccaatcgc accggacgct ctcccaagga caagttcatt gtgaaggatg cgaccacgga
     1741 gcacacggtc gcctggggcg ccgtcaacgc tgccatcgat ccggaaaagt tcgacgctct
     1801 ctatgcccgc gtcatggaac acctgcgcgg ccgcgaactt ttcgtgcagg atcttttctg
     1861 cggcgccgat cccgtctacc gcctgcccgt ccgcatcgtc aacgagtatg cctggcacaa
     1921 cctgttcgtg caccagcttt tcctgcgccc gacggccgcc gagctcaaga cgcatcatcc
     1981 cgagttcaca gtgatttccg ctcctgagtt caaagccgat cccaagcgcg acggcgtgaa
     2041 ctcggaagtc ttcatcctgc tgaacttcac ccggcgcacg gtgctgatcg gcggcacgtc
     2101 atacgcgggc gaaatgaaga aatcgatttt cagcgtaatg aactacattc tgccgcagcg
     2161 caacgtcttc cccatgcact gctcggccaa cgtgggccac gacggcgtga ccgcactgtt
     2221 cttcggcctc tccggcacag gcaagaccac gctttcggcc gatccctcgc gtgacctgat
     2281 cggcgacgac gagcacggct ggagcgccga cggcgtcttc aacttcgaag gcggatgcta
     2341 cgccaagtgc atcaagctct cgaaaaagaa tgaaccgcag atctggaacg cgctccgctt
     2401 cggctgcgtg ctcgaaaacg tcacgctcga cacgcacacg cgcgttcccg actacaacga
     2461 cgactcgaaa accgagaaca cacgcgcggc ctatccggtc gatttcatcg acaatgccgt
     2521 gatcccgggc gtcggcggac atccgaagaa cgtggtcttc cttactgccg acgctttcgg
     2581 cgtgctgccg ccgatctctc gcctcacgcc cgagcaggcg atgtatcact tcttctccgg
     2641 ctacacggcg aaggtcgcgg gaaccgaagc cggcgtgaaa gagccctcgg cgacgttctc
     2701 cacgtgcttt ggctcgccct ttcttccgct gcggccgaag gtttacgcgg aaatgctggg
     2761 ccggcgcctg cgcgagcacg acgcgcaatg ctggctggtc aacagcggat ggcagggtgg
     2821 tccctacgga gtgggcaagc gcatggagat tcccttcact cgcgccatgg tcgatgccgc
     2881 ggtcgagggc gagttaacca aagaagagtt cgagatcgaa ccgacgttcg gctttgcgat
     2941 tcccaaggcc tgtcccggcg tgcctccgca ggtgctg
//
LOCUS       DASPUL010000681         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_408037, whole genome shotgun sequence.
ACCESSION   DASPUL010000681 DASPUL010000000
VERSION     DASPUL010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408037"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(382..1158)
                     /locus_tag="VEJ00_15610"
     CDS             complement(382..1158)
                     /locus_tag="VEJ00_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02643.1"
                     /translation="MTQRQRAHERALEQAVSRALRDNLVPLQRTADELQQAYADLVAA
                     CSSSRPSNALPAMLRAQTAASALAAGLSVLSNFVAVALKPRDEGATEEESGTASAVAE
                     PEPEPESTTEVATTEPEGLTAKEELSAATEIPILAPVVEESPVVPEPARETPAFDVRS
                     LPTDLQELHRRANRVAKVAMQDIKLLRPKDVRLGRESKDICVRLRSDLDKARKEYDRR
                     FRTIQDHPVDYFHHWMVEILGEGDPEALGDYPYPSAALRQ"
     gene            1326..1829
                     /locus_tag="VEJ00_15615"
     CDS             1326..1829
                     /locus_tag="VEJ00_15615"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXX02644.1"
                     /translation="MLTAKEVCKLLKLEPLVAEGGFFAETYRSAHRLPKGALSNCYPG
                     ERSQSTAIYYLLTPETFSAMHRLRGDEVYHFYLGDPVEMLKLKPDGSAEALLLGQDIG
                     GGMRLQQVVCGGTWQGLRLAPGGSFALMGTTMAPGFDPADFELGRREELSAAYPAYAP
                     LIAMLTR"
     gene            2012..>2574
                     /locus_tag="VEJ00_15620"
     CDS             2012..>2574
                     /locus_tag="VEJ00_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02645.1"
                     /translation="MAAVVAHNPAQVKFSPEVKFVENTIPMKPGEGLWKTASALPTTF
                     KIYVPDPVSEEVGFMGVMKESDKPVLLGLRLKVRNGQITEAEHLIARNLSATSLANLE
                     TPRPGLLATVPPAERVPRAEMLKIGLSYYDALVNGDGKAAPFADDCLRHENGMQTTGN
                     PPPATPGFATMGAMGCSAQLDTKIFNY"
BASE COUNT          513 a          741 c          783 g          537 t
ORIGIN      
        1 tgtgccagat gggtctcggc ggtccagtac agagcataat ccgcaagcaa aggatcgttc
       61 tttgcgcgcg caagccattg aagcgcctgc gtgtagtgct ccttgctgta gtcgaaataa
      121 cccagtgcga gggccgcgcg cacgcctaga gtgccagatg acttctgcat cgccatggca
      181 ctcagctgtg cgtaggctcg aggcgcagtc gttcccttca gagatcgcgc cagcttctca
      241 agttgatctt ctgtgaatac aggcttttga acagaaggcg atggtgagct ggacccggag
      301 ccagccatac acgccgatga caccagcagc aatacgccaa gcgtggcaac aatccggctg
      361 gggagtctca tgccggaaat attattgccg aagggccgca gagggatagg gatagtctcc
      421 gagcgcttcg ggatcgcctt ctccgaggat ttcaaccatc caatgatgaa agtaatcgac
      481 cggatggtcc tgaatggtgc ggaaacgacg atcgtattcc tttcgggctt tgtcgaggtc
      541 acttcgcaac cttacgcaaa tgtctttgct ttcacgaccc agacgaacgt ctttgggccg
      601 caatagtttg atgtcttgca tggctacttt ggctacccgg ttggcccggc gatgcagctc
      661 ctgaagatcg gtcggaagac ttctgacgtc aaaggcgggt gtttctcgcg cgggctccgg
      721 cacgacgggc gattcctcca ccactggggc gaggatcggt atttcagtcg cggcagaaag
      781 ctcctccttg gctgtaagac cttctggctc ggtcgtagct acctcagtgg tgctttcagg
      841 ttcgggttcg ggttcggcta cggccgatgc ggtgcccgac tcttcctccg tcgcaccttc
      901 gtctcgtggc ttgagcgcca cggcgacgaa attggagagc accgacagcc ccgcggcgag
      961 ggccgaggca gccgtctggg cacgtagcat ggctggaagg gcattgctgg gacggctgga
     1021 ggagcaggcg gcgaccagat ccgcgtacgc ctgctgcaac tcgtcggctg tgcgctgcag
     1081 cggaacgaga ttgtcgcgca aggcccggga gacagcctgt tccagggcac gttcgtgagc
     1141 tcgctggcgt tgtgtcaagg agtttccgcc attcgcgatt atggaaacgc cctcaggacg
     1201 tgtcaactgg cattcagacg gtacgttccc tagcgtacag cagccgattc ggtgttttcg
     1261 gcattctcgt tgggccgcga ggtttcactt gcgttcgagg acagtcatcc gtatcttcgc
     1321 agcgcatgct gacggccaag gaagtctgta aactgctgaa gctggaaccc cttgttgcag
     1381 aagggggctt tttcgcggaa acctataggt ccgcacaccg actaccgaag ggtgcgcttt
     1441 ccaactgcta tccaggcgag cgatcgcagt ccacggcgat ctactacctg ctcactcccg
     1501 agacgttttc ggcgatgcac cgcctgcggg gagacgaagt gtaccacttc tatctgggtg
     1561 accccgtcga gatgctgaaa ctcaagcccg atggctcggc ggaagctctc ttgctcgggc
     1621 aagacatcgg cggaggaatg cgtctgcagc aggtcgtgtg tgggggaact tggcagggct
     1681 tgcgacttgc gcccggcggg agttttgcgt taatgggcac gaccatggca ccgggtttcg
     1741 accccgccga cttcgagctg ggcagacgcg aagaactaag cgctgcctac ccggcgtatg
     1801 cgccgctgat cgccatgctc acccgctgac ggctaggcga ccgctgttcg ggtttgcgcg
     1861 gatgtcaaaa agggagatta aagtgaagac cttcggtcgg atggctctga tccttctcgt
     1921 tttgagcggc gtttcgacgc ttccacgacc atcgcaggcg gctgcggccc aatgtaatcg
     1981 agtctgtttg attaacacgg tcggtacgta cttggccgcc gttgttgcgc acaatcctgc
     2041 acaagtgaaa ttctcgccgg aggtgaagtt tgttgagaac acgattccca tgaaaccggg
     2101 cgagggtctc tggaagaccg cgtccgcgct gccaacgact ttcaaaatct atgtacccga
     2161 tccggtctca gaggaagtcg gtttcatggg cgtcatgaaa gagagcgaca aacccgtgct
     2221 gctgggctta cgcctaaagg tccgaaacgg gcagatcacc gaagccgagc acctgatcgc
     2281 tcgcaacctc agcgccacga gtctcgcgaa cctggaaacg ccacggccgg gcctgctggc
     2341 aactgtaccg ccggccgaac gagtgcctcg ggcagaaatg ctgaaaatcg gtctctccta
     2401 ctacgacgcc cttgtgaacg gcgatggaaa ggcagcgccg tttgccgatg actgcctccg
     2461 ccacgagaat gggatgcaaa ccaccggcaa tcccccgccg gccactcccg gtttcgcaac
     2521 gatgggcgcg atgggctgct ccgctcaact ggacaccaag atctttaact acat
//
LOCUS       DASPUL010000682         8775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_816856, whole genome shotgun sequence.
ACCESSION   DASPUL010000682 DASPUL010000000
VERSION     DASPUL010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816856"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1447
                     /locus_tag="VEJ00_15625"
     CDS             <1..1447
                     /locus_tag="VEJ00_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02646.1"
                     /translation="TQPYASDIDHTGALSEAIVTLPQPVAPKGTVDLEFGYEGVIVLD
                     ATRLTRIGTPEDEAKSSDWDQIGADFTAVRGAGYVAWYPIATESANLSEGSNMFEALG
                     RWKTREADSQMSVLFESTRKSPILFSGTPNLFAVTPEEGMVKVCAFSMIRPGWNVPTF
                     VQADYTKVDVKGASAVEYLPGKEGAALSYAEVLGTLDPLSSAQGPAGIQVAQSADPDA
                     AAFASQGLLLIPFKPAASNEDRMTLVYALARQKAVSPQPWISEGLAHLAQVLDVEHQY
                     GRKAALEYLNSHQSMTVQSEKPASRETEHSSSSHALTNAADEVYLQSKAMYTWSMLRD
                     MVGDVVISSVLLSYHAMDDKDPAYMQTLFARHTQRDLQWFFEDWVYHDRGLPDFKVES
                     VFPLKTATESYLVTVTVDNLGTAGAEVPVIVKFAGGEVSKRVEVRAKSKGVIRVEVPK
                     PPLEATVNDGSIPESDVTNNTFSVQPTQPPK"
     gene            1547..2977
                     /locus_tag="VEJ00_15630"
     CDS             1547..2977
                     /locus_tag="VEJ00_15630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXX02647.1"
                     /translation="MAYIQFLGAAGTVTGSKHLINTGDPTGKIGFQVLIDCGLFQGQK
                     EWRERNWRDTPVPAKEIDAVILTHAHLDHCGWIPRLVKEGFRGPIYATSPTIDLCGII
                     LPDSGHLQEEDAHFYNKGKKSKHDPALPLYTFEEAQNSLQYFKPVQVGQTIPLSAGLS
                     FRFVRAAHILGSCMAEVALQTDGQARRLLFTGDIGRVLDHKVAPGKVVHSGPAEGEIA
                     DLLVMESTYGNRTHPKEDPLPELAGLITDTAKRGGSVIVPAFAIERTQKFVFFLKHLI
                     EDGKIPRVPVFCDSPMAIKAVEVFLKHREEYSQETCDMIRKYGSPLEWPGFTFASTPE
                     ESKRINAVLTPCIIISSSGMVTGGRILHHLAQRLPDPKNLVLFIGFQAPGTRGFSIKS
                     KADEVKIYGDYVPIRAQVAALEQFSDHADPPELLEWLHTFGKQPATTYLVHGESDAAG
                     QLRELMTKELGWNVKVAAYMEKVEVK"
     gene            complement(2992..4083)
                     /gene="trpD"
                     /locus_tag="VEJ00_15635"
     CDS             complement(2992..4083)
                     /gene="trpD"
                     /locus_tag="VEJ00_15635"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HXX02648.1"
                     /translation="MILDALHSLANHSQSLDRAAAREVMAEVLTGKCTDGQIAGLLIA
                     LRMKGETVEEIVGFAEAIRAAAAPLPIERATSNGGAADALAVSGTGRDALFEEESSLV
                     DTSGTGGDASGTFNISTATALVTGGAGVRVAKHGNRSISSKCGSADVVEALGVNIQLS
                     PERAAQCLREVGICFLYAPNLHPAMKQVQGVRRELRMRTMFNLLGPLTNPARASGQVV
                     GVYSLDLVEKLAEALSMLGLRRALVVHGLDGLDEITITGTTRVAEAREGSVRSYEVEP
                     EEFGMRRATLQEIAGGDANENAAIIRAILEGERSPRRDVVLLNAAAALVAAGRAEHIA
                     EGIPLAARSIDSGSAAKKLEGLMRFASTE"
     gene            complement(4125..4751)
                     /locus_tag="VEJ00_15640"
     CDS             complement(4125..4751)
                     /locus_tag="VEJ00_15640"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HXX02649.1"
                     /translation="MAYKGETINSGIFKQAIAGPVQLRTLNLDGDRQADLSVHGGPYK
                     AVYAYPSEHYAFWRQELPGMDLPWGTFGENFTTEGLLENDLHIGDRLRIGTAVIMVRQ
                     PRVPCYKLAAKFQRDDMIDRFLASGRSGFYFSVEQEGAVTAGDSFDLLSREPEAMTID
                     EMNQLLVRDRYNCAMIEKALATAALPESWRDYFSRRPASAGNAEIDAR"
     gene            5016..6212
                     /gene="sucC"
                     /locus_tag="VEJ00_15645"
     CDS             5016..6212
                     /gene="sucC"
                     /locus_tag="VEJ00_15645"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HXX02650.1"
                     /translation="MKIHEYQAKGILKKYGVTVPRGAMAASREEAESVAKQLFSEGAA
                     GVVVKAQIHAGGRGKGGGVKIAKSVDEAGELAAKILGMRLITHQTGPEGRIVRRLLIE
                     ETLPIEKELYLGILVDRAEGKPVFMASAAGGMEIEQVAAENPDAILKQHIEPGMDLEA
                     FQARKIAFKLGLKAQQINPAVQFLTSLYKAFLDTDASLLEINPFITTTDGRLFALDAK
                     INFDDNALFRHADLRELRDITEEDPLEVEASKYSLNYIKLDGNVGCMVNGAGLAMATM
                     DIIKYAGGMPANFLDVGGGANAEQVAHAFEILLSDKNVKAVLINIFGGILRVDTLATG
                     VVEAARKTNIKLPVVLRLEGTNVDEGKRILNSSGLNFVVTDTMKDAADKVVAAARGTL
                     GVTEWR"
     gene            6296..7174
                     /gene="sucD"
                     /locus_tag="VEJ00_15650"
     CDS             6296..7174
                     /gene="sucD"
                     /locus_tag="VEJ00_15650"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXX02651.1"
                     /translation="MAILVDKSTRVLVQGLTGREGTFHAKASAAYGTQIVGGVTPGKG
                     GTTHEGWPIFNTVREAADQTGANATVIFVPPPFAADAIMEAADAELDLIVCITEGIPT
                     LDMVKAWEFLQTRRSRLIGPNCPGIISPGKCKIGIMPASIHKEGSIGIVSRSGTLTYE
                     AVHQLTQRGIGQSTAIGIGGDPIIGTNFVDALDLFNRDPDTEAVILIGEIGGNAEETA
                     AEFVKTHMKKPVVGFIAGQTAPPGRRMGHAGAIISGGKGTAAEKIKALEAAGIKVAKS
                     PAAIGETLAAAVGVSV"
     gene            7194..7757
                     /locus_tag="VEJ00_15655"
     CDS             7194..7757
                     /locus_tag="VEJ00_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02652.1"
                     /translation="MMKRPDCFLDSVRRNFDWLIEACGMHCGPEERTVVEYRSEHVVL
                     KLVYDERNSRQVNAFVSCPQITRVPLELFLILSAYGYEGADEIGNLRATTPEELERAC
                     SRLAELIREYALRILRGDRDEILFVSTFVKWRSVKYAWEGNNTKSILAAYPGMISSPY
                     FDRVKATLSAEDIAILEKVRRICEDAK"
     gene            7791..8216
                     /gene="ndk"
                     /locus_tag="VEJ00_15660"
     CDS             7791..8216
                     /gene="ndk"
                     /locus_tag="VEJ00_15660"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXX02653.1"
                     /translation="MKTLQRTFTIVKPDAVRKNAVGDIIEQFEKNGFRILAMKMLEIS
                     KPQAEQFYAVHASRPFFNSLTDFMSSGPIVVLALEKENAISDLRKLMGATNPAQAEEG
                     TIRKKWATNIEHNAIHGSDAEDTARFELSFFFAGYELAK"
     gene            8279..8467
                     /locus_tag="VEJ00_15665"
     CDS             8279..8467
                     /locus_tag="VEJ00_15665"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF002571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HXX02654.1"
                     /translation="MPMVQITMLAGRTADQKRKIAKRITDALAEEAGAKREAIIIAFH
                     EVSKESYAHGGELLIDRK"
     gene            complement(8530..>8775)
                     /locus_tag="VEJ00_15670"
     CDS             complement(8530..>8775)
                     /locus_tag="VEJ00_15670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HXX02655.1"
                     /translation="ETEVAALFEKLTEQSRKEPGCAMYQVHRHKTEPRRFFIYELYKD
                     DAALEAHRTAPHFLQFARKDLPKVADRVEGHLFEPLG"
BASE COUNT         1857 a         2558 c         2667 g         1693 t
ORIGIN      
        1 cacccagcct tacgcatccg acattgacca cacgggcgca ctttccgaag caatcgtgac
       61 cttgccacag ccggtcgcgc ctaaaggaac tgttgacttg gagtttggct acgaaggcgt
      121 gatcgtgctg gatgcaacgc ggctgacgcg catcggcacg ccggaggatg aagcgaagag
      181 ctccgactgg gaccagatcg gtgcggactt cacagccgtg cgcggcgccg ggtatgtcgc
      241 gtggtatccg atcgcgacgg agtcggcgaa tctttccgaa ggcagcaaca tgtttgaggc
      301 gctgggaagg tggaaaacgc gggaagctga ctcacagatg tcagtgcttt ttgaatcgac
      361 gagaaagtcc ccgatcctgt ttagtggcac gcctaacctt ttcgcggtca ctcccgagga
      421 agggatggta aaggtctgcg cctttagcat gattcggcct ggctggaacg taccgacctt
      481 cgtccaggcg gactacacaa aagtggatgt gaaaggcgca tctgccgttg agtaccttcc
      541 gggcaaagag ggcgcagcgc tgtcctacgc cgaagtcctc ggaacattgg atccgctctc
      601 ctccgctcaa ggtcccgcag gaatccaagt ggcacaatcg gccgacccgg atgcagccgc
      661 cttcgcgagc caaggattgt tgttgatccc tttcaagccg gcggcctcga atgaggaccg
      721 aatgacgttg gtctacgccc ttgcgcgtca gaaggctgtt tctccgcagc catggatcag
      781 cgaggggctt gcccacctcg cacaggtgct ggatgttgaa catcagtacg ggcgcaaagc
      841 ggcgctggag tacctcaact cccatcaatc catgactgtg caatcggaaa agccggcttc
      901 tcgggaaacc gagcattcct cttccagcca cgctctcacc aacgcggccg acgaagtgta
      961 tctgcaaagc aaggccatgt acacgtggtc gatgctgcgc gacatggtgg gggacgtcgt
     1021 cataagttct gttcttcttt cttaccatgc aatggacgac aaagatcccg cgtacatgca
     1081 gacacttttt gcgcggcaca cccagcgcga cctgcagtgg ttcttcgagg actgggtcta
     1141 ccacgatcgg ggtctgccgg acttcaaggt cgaatctgtc tttccgctca agacggcaac
     1201 ggagtcatat ctcgtcacgg ttacggtaga caaccttggc acggctgggg cagaggtgcc
     1261 agtgatcgtg aagtttgctg gcggggaagt tagcaagcgc gtcgaagtgc gagccaagag
     1321 taaaggcgtc attcgagtgg aggtaccgaa gcctccactg gaagcaacgg ttaacgacgg
     1381 gagcatccct gagagcgatg tgacgaacaa tacgttcagc gtgcaaccga ctcaacctcc
     1441 aaaatgatcg tcggtttttc cgatcgcaag tcggcgcccc cggcaatccc agctctttct
     1501 ccgtgcctct atggtaggtt tgatgctttc ggtttgagga ggaattttgg cctatatcca
     1561 gtttctcggg gctgccggca cggtcactgg ctcaaagcat ctcatcaaca cgggcgaccc
     1621 taccggcaag atcggatttc aggttctgat cgactgcgga ctgttccaag gacagaaaga
     1681 atggcgagag cgcaactggc gcgacacgcc ggttccagcc aaggagattg acgcggtcat
     1741 cctgacccac gcgcacctcg accactgcgg ctggatcccg cgcctggtga aggaaggttt
     1801 tcgcgggccg atttacgcga cttcaccgac catcgatctg tgcggcatca ttcttcccga
     1861 ctccgggcac ctgcaggaag aagacgccca tttctacaac aaggggaaaa aatcgaagca
     1921 cgaccctgcc ctgccgctct acacctttga ggaagcacaa aattccttgc agtatttcaa
     1981 gccggtccag gtgggacaga ccattccgct ctcagccggg ctttcgttcc gcttcgtgcg
     2041 cgccgcccac atcctcggtt cgtgcatggc cgaagttgcg ctgcaaaccg acgggcaggc
     2101 gcggcgcctg ctgtttaccg gcgacattgg acgcgtgctc gaccataaag tagcgcccgg
     2161 gaaagttgtg cattccgggc cggcggaggg cgagatcgct gatctccttg tgatggagtc
     2221 gacgtacggc aaccgaaccc atcccaagga agaccccctg ccggaactcg ccggtcttat
     2281 taccgatacc gccaaacgcg gaggcagcgt gatcgtaccg gcatttgcca tagagcgtac
     2341 ccaaaagttt gtgttctttc tcaagcatct gatcgaggat gggaagattc cacgcgtgcc
     2401 agtcttctgc gacagcccca tggcgatcaa ggcagtcgag gtatttctga agcaccgcga
     2461 ggaatacagt caagaaacgt gcgacatgat tcggaagtat ggatcgccgc tcgaatggcc
     2521 cgggttcacc ttcgcttcga ctccggagga atcgaagagg atcaatgccg tgctgacccc
     2581 gtgcatcatc atttcctcca gcggcatggt tacgggaggc cgcatcctgc atcacctcgc
     2641 gcagcggctg ccggatccga agaacctcgt gctgttcatc ggctttcagg caccgggaac
     2701 gcgcggcttt tccatcaaga gcaaggcgga cgaagtcaag atttacggcg actacgtgcc
     2761 gattcgcgcc caggtcgcgg cgctcgagca gttcagcgac catgccgacc cgccggagtt
     2821 gctcgagtgg ttgcacacct tcggcaagca gcccgcaacg acttatttgg tgcacggcga
     2881 atcggatgcc gccggtcaac tgcgcgaact gatgactaaa gaactgggat ggaacgtgaa
     2941 agtggctgct tacatggaga aagtggaagt caagtagctg cgggtccgca ttcactctgt
     3001 cgaagcaaac cgcatcaggc cttcgagttt cttcgccgcc gatccggagt caatggatcg
     3061 ggctgcgaga ggaattcctt cggcgatgtg ttccgccctt ccggcagcca ccaatgcagc
     3121 agcagcgttc agcagcacaa cgtcgcggcg cggcgatcgc tcgccctcga gaattgcacg
     3181 aatgatcgcc gcattctcgt tcgcgtcgcc gccggcgatt tcttgcagcg tggcgcgcct
     3241 catgccaaat tcttccggct caacttcata ggagcgaacc gagccttcgc gagcctcagc
     3301 tacccgagtg gttccggtga tcgtgatctc gtcgaggccg tcgaggccat gcactacgag
     3361 cgcccgccgc aaaccgagca tgctcagtgc ttccgccaac ttctccacca agtcgagcga
     3421 atacacgccc actacctgcc cggaagcccg cgcgggattg gtcaggggac caagcaggtt
     3481 aaacatcgtt cgcatgcgca actcgcgacg tacgccctgt acctgcttca tggccggatg
     3541 caggttcggc gcgtagagga agcagatgcc aacctcgcgc aggcactgcg ctgcgcgctc
     3601 cggtgatagc tggatgttca cgccgagtgc ttcgaccacg tctgcggagc cgcatttgga
     3661 gctgatgctt cgatttccat gcttggccac gcgtacgccg gctccaccag taaccagagc
     3721 cgtggcggtg gagatgttga acgtcccgct ggcatcgccg cccgtgccac tagtgtcgac
     3781 cagggacgac tcttcctcga acagagcatc gcgaccggtg ccgctgaccg ccagcgcgtc
     3841 cgcagcgccg ccattcgaag tggcgcgctc gatcggcaag ggcgccgcag cggcgcggat
     3901 ggcttcggcg aagcccacga tctcttctac ggtctcgcct ttcattcgca aagcaatcag
     3961 caatcctgca atctgcccgt cagtgcactt gccggttaga acctccgcca tcacctcgcg
     4021 cgcggcggcg cggtcgagcg attggctgtg attggcgagg gaatggaggg cgtcgaggat
     4081 catgcttcag gcggcattgt acggcatcgc ggcatcgaac cgtgtcaacg ggcgtcgatc
     4141 tccgcgttgc cggccgaggc cgggcgccgc gaaaagtagt cgcgccagct ttcgggcaac
     4201 gcagcggtcg ccagagcttt ctcgatcatg gcgcaattgt agcggtcccg cacgagcaac
     4261 tggttcattt cgtcgatggt catggcttcg ggttcgcggc tgagcagatc gaatgagtcg
     4321 ccggcggtca ctgcgccttc ctgctccacc gagaagtaaa acccgctccg cccgctcgcg
     4381 aggaagcggt caatcatgtc gtcgcgctgg aacttcgccg ccagcttgta acagggcacg
     4441 cgaggctgac ggaccatgat gacggcggtt ccaattcgca ggcggtcgcc gatgtgcagg
     4501 tcgttttcca ataaaccttc cgttgtgaag ttttcgccga acgtgcccca gggaagatcc
     4561 atgcccggca attcctggcg ccagaaagcg tagtgctctg agggatacgc gtagacggcc
     4621 ttgtagggcc cgccgtgaac gctaagatcg gcctggcgat cgccgtcgag gttcagggtg
     4681 cggagctgca ccggtccggc aatcgcctgc ttgaagatgc cggagttgat cgtctcgccc
     4741 ttgtatgcca tcagccgcgg gcgggctaca ttcagtgaga tgagcttcag cggtcctcca
     4801 ttcggtggcg tcgctgcgcc gccgcggtat cagacttcga tgatccgccc cgcgaaaagg
     4861 tgcagggcgg gctgtcgttt tgggactcac ggcggcaaag aaggccttcc gatgtttgct
     4921 caaccgtcgg tcgcttctat agaatcaggg gtttgctgcc cgctgtgcga cgccgcaagc
     4981 aggatccttc cgattcttac tggtgagctt cacttatgaa aatccacgag tatcaggcca
     5041 agggaattct caagaagtat ggcgtgaccg tgccgcgggg agcgatggcc gcgtcgcgcg
     5101 aggaagcgga gtccgtcgcg aaacaacttt tcagcgaagg cgcggccgga gtcgtggtta
     5161 aggcacagat tcatgcggga ggccgtggca agggcggtgg ggtaaaaatc gcgaaatcgg
     5221 tcgatgaagc aggcgaactc gccgccaaga tcctgggcat gagactgatc acgcaccaga
     5281 ccggcccgga aggccgcatc gtgcgccgct tgctgatcga agaaacccta cctatcgaga
     5341 aagagctcta tctcggcatt ctcgtggatc gcgccgaagg caagccggtg ttcatggcct
     5401 cggccgccgg cggcatggag atcgagcagg tggcggcgga gaatccggac gcgattctga
     5461 agcagcacat tgaacccggg atggacctcg aggcatttca ggcgcgcaag attgccttca
     5521 agctgggcct gaaggcgcag caaatcaatc ccgcagtaca gtttctgacc agcctgtaca
     5581 aagctttcct cgacactgac gcttcgctgc tggaaattaa tccgttcatc accaccaccg
     5641 acggaaggct gttcgcgctc gacgcgaaga tcaacttcga tgacaacgcc ctgttccggc
     5701 acgccgactt acgcgaactg cgcgatatca cggaagagga tccgctcgag gtcgaggctt
     5761 cgaagtacag cctcaactac atcaagctcg acggcaacgt cggatgcatg gtgaacggcg
     5821 ccgggctggc catggcgaca atggacatca ttaaatacgc cggcggcatg cccgcgaact
     5881 tccttgatgt tggcggcgga gcgaacgccg agcaggtggc gcacgcgttc gaaatcctgc
     5941 tgagcgacaa gaacgtgaag gccgtgctga ttaacatctt cggcggcatc ctgcgcgtgg
     6001 atacgctggc cacgggcgtg gttgaagccg cacgcaagac caacatcaag ctgccggtgg
     6061 tgctgaggct ggagggcacc aacgtcgacg aaggcaagag aatcttgaac agctcagggt
     6121 tgaatttcgt cgtcaccgac actatgaaag acgcggccga caaggtggtc gccgcggcca
     6181 gagggacgct cggcgtgacg gaatggcgct aagtggtggt ggaagagcgg ccctttaggg
     6241 ccgcgtaagt gggcgggttc cagacggggc tttagccccg gtgctgtgag gtgttatggc
     6301 aatcttggtc gacaagagta ctcgggttct agtgcagggt ctcacgggcc gcgaaggcac
     6361 ctttcacgca aaagccagcg ccgcctatgg cacacagatc gtcggcggcg taacgcccgg
     6421 caaaggcggg acaacgcacg aaggctggcc gattttcaac accgtgcggg aggctgccga
     6481 tcagaccggc gccaacgcaa cagtgatttt cgttccgccg ccgtttgcgg ccgacgccat
     6541 catggaagcg gccgatgccg agcttgatct gatcgtctgc atcaccgaag gcattccgac
     6601 gctggacatg gtcaaagcct gggaatttct gcagacccgg cgctcgcggc tcatcgggcc
     6661 gaactgcccg gggatcattt caccgggcaa gtgcaagatc ggcatcatgc cggcatcgat
     6721 ccacaaggaa ggatccatcg gcatcgtttc gcgttcgggc acgctgacgt acgaggcggt
     6781 acatcagctc acgcagcgcg gcattggaca atcgaccgca attggcattg gtggcgatcc
     6841 gatcatcggc acgaattttg tcgatgcgct cgatcttttc aatcgcgatc ccgataccga
     6901 agccgtgatc ctgatcggcg aaatcggagg caatgccgag gaaaccgcag ccgagttcgt
     6961 gaagacccac atgaagaagc cagtcgtggg attcatcgct ggacagaccg cgcctcctgg
     7021 acgccgcatg ggccacgccg gagccatcat ttccggcggc aaaggcaccg ctgcggagaa
     7081 gatcaaggcg ctcgaagcgg cgggcatcaa agttgccaaa tcgcccgcag ccatcggcga
     7141 aacgctggca gcagctgtgg gtgtgagcgt gtagtgcggc ggagcgcccc gcaatgatga
     7201 agaggccgga ttgcttcctg gattctgtac gcagaaactt cgattggctg atcgaggcct
     7261 gcgggatgca ctgcggcccc gaagaacgca ccgtcgtcga gtatcgtagc gaacacgttg
     7321 tgctcaagct agtttatgac gagcgcaatt cgcgacaggt gaatgccttc gtcagctgcc
     7381 cgcaaattac gcgtgtgccc ttggaattgt ttcttattct cagcgcgtat ggctacgaag
     7441 gagcagacga gatcggcaat ctcagggcga cgactccgga ggaattggaa cgggcctgct
     7501 cgcgcttagc ggaactcatc cgagagtatg cgctcagaat tctgcgcggt gaccgggacg
     7561 aaattctatt tgtgtcgacg tttgtgaaat ggaggagcgt gaaatacgcc tgggagggta
     7621 acaatacgaa gtcaatcttg gccgcttatc caggaatgat atctagcccc tacttcgatc
     7681 gcgtcaaggc tactctcagc gcggaagata tcgctatcct cgaaaaagtt agacggattt
     7741 gcgaagatgc caaataatgc attcattccc gagaggaata actatcgatc atgaagactc
     7801 tgcaacgcac ctttaccatc gtcaaaccgg acgccgtgcg caagaacgcc gtcggcgaca
     7861 tcattgagca attcgagaag aacggattcc gcatcctcgc catgaagatg ctggaaatct
     7921 ccaagcccca ggccgagcag ttctacgccg tacatgccag ccgcccgttt ttcaactcac
     7981 tcaccgattt catgtcgagc gggcccatcg tcgtgctcgc gctggagaaa gaaaacgcca
     8041 tctccgacct gcgcaagctc atgggtgcga cgaatcccgc ccaagctgag gaaggaacga
     8101 ttcgcaagaa gtgggcgacc aacatcgagc acaacgctat ccacggctcg gacgcggaag
     8161 acacggcgcg ctttgagctg agcttctttt ttgcaggata cgaattggca aagtgacgcc
     8221 gtttagcgag gcgtctgagc ctcgctggcg tcatcctgag cgaagcgaag gatctcctat
     8281 gcccatggtt caaatcacga tgctcgcagg ccgcacggcc gatcagaagc gcaagatcgc
     8341 gaaacgcatt acggatgcct tggcggaaga agccggggcg aagcgggaag cgatcatcat
     8401 cgcttttcac gaagtgtcga aggaaagcta cgcccacggc ggtgagttgc tgatcgatag
     8461 gaagtgatcc tggatcgctt tgctgctgcc ttccgggcag gaacgcgggc gggagcgtcc
     8521 gcggcacact catcccagcg gctcgaacaa gtgcccttcc acgcggtcgg cgaccttggg
     8581 gagatctttc ctggcgaact gcaggaaatg cggcgccgtg cggtgggcct cgagagcggc
     8641 gtcgtccttg tacagttcgt agatgaagaa gcgacggggc tcagtcttgt gccgatgcac
     8701 ctggtacatg gcgcagcccg gttccttgcg ggattgctcg gtcagcttct caaacagcgc
     8761 ggcaacttcg gtttc
//
LOCUS       DASPUL010000683         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1911867, whole genome shotgun sequence.
ACCESSION   DASPUL010000683 DASPUL010000000
VERSION     DASPUL010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1911867"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1044)
                     /locus_tag="VEJ00_15675"
     CDS             complement(43..1044)
                     /locus_tag="VEJ00_15675"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXX02656.1"
                     /translation="MGFIFLRYIWLIPLLPAFGATVMFFFGRKLQKSSVSAVCVGVVV
                     LAFLLACGCVWQYTNWAAANHQPYESIGYTWLGSGTGHLTYTTQGGSQAPFNLDARLL
                     LDPLSSIWLLFVTGVGMLIHIYSIGYMGHEGGYYRFFGYLNLFMFSMLTLVLANNYAL
                     MFVGWEGVGLCSYLLIGFYFQRKSASDAANKAFIVNRIGDAGFILGMFFIAWICGSLR
                     FVDVTGFAREHAAGLMGNSFITAATLLLFVGACGKSAQLPLYVWLPDAMEGPTPVSAL
                     IHAATMVTAGVYMVARSNALFVLAPDSMLIVAIVGALTAIFAASIGLVQNDIKRVLA"
     gene            complement(1133..1459)
                     /gene="nuoK"
                     /locus_tag="VEJ00_15680"
     CDS             complement(1133..1459)
                     /gene="nuoK"
                     /locus_tag="VEJ00_15680"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HXX02657.1"
                     /translation="MLDISTLHYLVVGAALFLLGVIGVLTRRNVVIVLMSIELILNAV
                     NLNLVALSRYWQGMHGPASLHGQVFAIFVITDAAAEAAVGLGILIAFFRNRETVNVDE
                     VNLLKW"
     gene            complement(1544..>2129)
                     /locus_tag="VEJ00_15685"
     CDS             complement(1544..>2129)
                     /locus_tag="VEJ00_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HXX02658.1"
                     /translation="GRRGAPPMNPIAPPNPPVATPFFFYLLSGTMILGGILVITRKNA
                     VHSALALITTLLAQAGLYLMLYAPFVAGVQIILYAGGIMVLFLFVIMLINIERNMKER
                     QFNKQWLVGIAAAAALGGLFIAVYTKGATLFSSGRFAYRDPASLYAESGNTQGVAALL
                     FDQHTGMYMFAFEIASLLLLVAIIGAVVMAKKRI"
BASE COUNT          502 a          618 c          640 g          369 t
ORIGIN      
        1 accgagggca aggaacatgt agccgagctg cgaaacggtc gactaggcca gcacccgctt
       61 gatatcgttt tgcacgaggc cgatcgaagc cgcgaagatc gccgtcagtg cgcccacaat
      121 ggcaacgatc agcattgaat ccggagcgag cacaaacagg gcgttcgatc tagcgaccat
      181 gtacaccccg gctgtcacca tggttgccgc gtggatcagc gcggagactg gcgtggggcc
      241 ttccatggcg tcgggcagcc agacatagag tggaagctgc gccgatttgc cacaggctcc
      301 aacgaaaagc agcagcgtcg cggcagtgat gaaggaattg cccatgaggc cggcggcgtg
      361 ctcgcgggca aatcctgtga cgtcgacgaa gcgcagcgac ccgcaaatcc aggcgatgaa
      421 aaacatgcca aggatgaatc cggcgtcgcc gatgcgatta acgatgaacg ccttattggc
      481 tgcatcgctg gccgacttgc gttgaaaata gaaaccgatc agcagatacg agcagagccc
      541 gacgccttcc cagcccacaa acatcagcgc gtagttgttg gccaacacca gcgtcagcat
      601 ggagaacatg aacaggttca ggtagccgaa gaagcggtag tagccgcctt catgccccat
      661 gtatccgatg gaatagatgt ggatcagcat gccgacgccg gtgacaaaca gcagccagat
      721 gctggagagc gggtcgagaa ggagccgggc gtcgagattg aacggggcct gggaaccgcc
      781 ctgggttgta tacgtcagat ggccggtacc actgccgagc caagtgtagc cgatactctc
      841 gtagggctgg tggttggccg cagcccagtt cgtgtactgc cacacgcagc cgcaggcgag
      901 caggaaggcc agcaccacca cgccaacgca gaccgcgctg accgaagatt tctgcagctt
      961 gcggccgaag aagaacatca ccgtggcgcc gaaggccggc agcagcggga tcagccatat
     1021 gtatcttaaa aagatgaaac ccatgtcgtt cgtcgtcatc ggtacgcggc cctaaagggc
     1081 cgctcttcca cgtagtaccc aggacatcta cggcacggct gaagccgtgc ccttaccact
     1141 ttagtaagtt cacttcgtcc acgttgaccg tctccctgtt gcggaagaag gcgatcagga
     1201 tgccgaggcc gaccgcggct tctgcggctg cgtcggtaat tacgaaaatc gcgaacacct
     1261 gtccgtgcag tgaagccggc ccatgcattc cctgccaata gcgggagaga gccaccaggt
     1321 tcagattgac ggcgttcaga atcagttcga tggacatcag cacgattact acgttccggc
     1381 gcgtgagcac gccgatcacg cccagcagaa acagggcagc gccaaccacc aggtagtgta
     1441 gcgtcgagat gtcgagcatc gcttgcattg aactgctctt ccggggctaa agcccgccta
     1501 ttttttggct ccatacgcgg ccctgaaggg ccgctcttcc acatcaaatc ctcttcttcg
     1561 ccatcacgac ggcgccgatg attgccacca gcagcaacag cgacgcgatt tcgaaggcga
     1621 acatgtacat cccggtgtgc tggtcaaaaa gcaaggcggc cacgccctgc gtatttcctg
     1681 attctgcgta gagcgacgcc ggatcgcggt aggcgaaccg gccggaggag aacaacgttg
     1741 cccctttggt ataaacggca atgaacaggc cgcccagagc ggccgccgcg gcaatgccca
     1801 ccagccactg cttgttgaac tggcgttcct tcatgttgcg ctcgatgttg atcagcatga
     1861 tcacaaacag aaacagcacc atgatgccgc cggcatagag aatgatttgc acgccggcga
     1921 cgaacggagc gtagagcatc aggtaaagcc cggcctgggc gagcaaggtc gtgatcaggg
     1981 caagggccga atgcacggca ttcttgcgcg ttatgaccag gatgccgccg aggatcatcg
     2041 tcccggagag caggtaaaag aagaacggcg ttgcgactgg cggatttggt ggcgctatcg
     2101 gattcatagg cggtgcaccg cgtcttcca
//
LOCUS       DASPUL010000684         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1091774, whole genome shotgun sequence.
ACCESSION   DASPUL010000684 DASPUL010000000
VERSION     DASPUL010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091774"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="VEJ00_15690"
     CDS             complement(<1..786)
                     /locus_tag="VEJ00_15690"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXX02659.1"
                     /translation="MLEQTISHYRIVEKLGGGGMGVVYKAEDTRLGRFVALKFLPEDL
                     AQDRSALERFRREAKAASALNHPNICTIYDIGEEDGRTFLVMEFLDGATLKHLIGNRA
                     MELETILSLGIEVADALDAAHAGGIVHRDIKPANIFVTKRGHAKILDFGLAKVKAAPS
                     VSGEVADVTAPTSVAVEEHLTSPGSTLGTVAYMSPEQAKGKELDSRTDLFSFGVVLYE
                     MATGTLPFRGETSALIFQAILDRAPVSPIRLNPDLPPRLEDVIN"
     gene            1339..2697
                     /locus_tag="VEJ00_15695"
     CDS             1339..2697
                     /locus_tag="VEJ00_15695"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HXX02660.1"
                     /translation="MTTKTMTAIGPKIKTALPGPNAKRALEGDQKFISPSYTRSYPMV
                     AKSGRGIVVTDVDGNEFFDFSAGIAVTSTGHCHPHVVAAIQKQAGELIHMSGTDFYYE
                     SMVELAGRLSKIAPMPGPHRIYYGNSGAEAIECALKLARYHTKRQNIIAFFGAFHGRT
                     MGALSLTASKPQQRRRFAPLVPGVTHVRYPDVYRGCVGGAQEAEKFALGCARFIEEKL
                     FKTTLPPEEVAAIFVEPIQGEGGYVVAPTVFMQELRRICDRHGILLVADEVQSGAART
                     GKWWAIEHSGVEPDIVCMAKGIASGMPLGITMSRAEIMDWVPGSHASTFGGNPVAIAA
                     ALATLDVIEKEHLLDNAASVGGHILNRMADWPATLKLVGDVRGRGLMIGVDIVKDKVT
                     REYGAVERDRIVEKAFEHGVLFLGCGPSSIRLCPPLVVTKEEADVAVDVLEECIAAVG
                     KA"
     gene            complement(2847..>3240)
                     /locus_tag="VEJ00_15700"
     CDS             complement(2847..>3240)
                     /locus_tag="VEJ00_15700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02661.1"
                     /translation="LARWWKRPQVLSCYLRDLAPLMAIDGRLGGLYVTRCAGFNLNKT
                     KNIGVPPDEVDLSRAARRAKVASHDHITQLAQVEVSILFASCAGPQVPRLFIRWKYTL
                     CQPIEAPDNPSREEGGEHCASVAASMHA"
BASE COUNT          608 a         1001 c          993 g          638 t
ORIGIN      
        1 gttgatgaca tcttccagtc tggggggcaa atcgggattc agccggatcg gcgagaccgg
       61 ggcgcgatcg agaatggcct ggaagatcaa tgctgacgtt tcgccgcgga acggcaacgt
      121 cccggtcgcc atctcgtaga gcaccacgcc gaacgaaaac aggtcggtgc gcgagtcgag
      181 ttcctttccc tttgcctgct ccggcgacat gtaggcaacc gtacccagag tcgagccggg
      241 gctggtcagg tgctcttcga cggctacgga agtcggcgcg gtcacgtcgg ctacctcgcc
      301 cgataccgaa ggagccgctt tgaccttggc taacccgaaa tcaaggatct ttgcatgtcc
      361 gcgcttggta acgaagatat tcgcaggctt gatgtcgcgg tgcacgatgc cgccggcgtg
      421 tgcggcgtcg agcgcgtcgg ccacctcgat tccaagcgag agaatcgtct ccagctccat
      481 cgcgcggttg ccgatcaggt gcttgagcgt cgccccgtcg aggaactcca tgacgaggaa
      541 cgtgcggccg tcttcctcgc cgatgtcgta gatcgtgcag atgttgggat gattcagcgc
      601 cgacgccgcc ttggcctcgc ggcgaaaacg ctccagagcg gaccgatcct gcgccaggtc
      661 ttcgggaagg aacttgaggg caacgaagcg gccgaggcgt gtgtcctcgg ccttatagac
      721 gacacccatc ccgccaccgc cgagcttctc aacgatccgg taatgggaaa tcgtctgttc
      781 gagcaaagac aggggtcctg ggcccgtggg gagaaatgct acggctgaag cccacggaag
      841 tcaaagtcca catttccggt gcccaaccga tcccaaatcc ggtgtcatcc tgagcggagc
      901 cgtttttcag gcggagcgaa ggatctccca tagcacgtcg atccagggag atccctcgcc
      961 ccgctggtga aagcgcgggg cttcgggatg acgccgttgg agaacgtcct ctcgttacca
     1021 aagggggttc cgccgccgta acacttccag ctataattag tcagtgatgt tgacggtttg
     1081 cgatgctgca gaagcgttcc aactcgcatc ctctctcgcg ccggtccgag tgctggctgc
     1141 actgtctggt gtgtccgcgg cgctatcgtg ccaatctgcc ctcgcgtctg cgtcccaatc
     1201 tcttcggtag cgactaactc cttcctcctc ctgaccttct cggtctgatg tgtgcttcaa
     1261 ccagagccac gtgcgccgag atcgtgttca ccgacaaccc ggcggctgac tactggcagc
     1321 tgccccacgg aggaagccat gaccaccaag accatgaccg ccatcggtcc caagatcaag
     1381 accgccctgc ccgggcccaa cgccaagcgc gcgctcgagg gcgatcagaa gtttatttcg
     1441 ccgtcctaca cccgctccta cccgatggtg gccaagagcg gacggggcat cgtcgtcacc
     1501 gacgtcgacg gcaacgagtt ttttgatttt tctgccggca tcgccgtgac ttcgaccggc
     1561 cactgccatc cccacgtggt cgctgccatt caaaagcagg cgggcgaact catccacatg
     1621 tcaggaaccg atttctacta cgaaagcatg gtggaactgg cagggcggct ctcgaagatc
     1681 gcgcccatgc ccggcccgca ccgcatctac tacgggaact cgggagcgga ggcgatcgag
     1741 tgcgcgttga agctggcgcg ctaccacacc aagcgccaga acatcatcgc gttcttcggc
     1801 gcgtttcatg gacgcactat gggcgcgctg tcgctgaccg cgtcgaagcc gcagcagcgg
     1861 cgccgttttg caccgctggt ccccggcgta acgcatgttc gctatcccga cgtgtaccgc
     1921 ggctgcgtgg gcggcgcgca ggaggccgag aaatttgcct tgggctgcgc ccgtttcatc
     1981 gaagaaaagc tgttcaagac cacgctgcct ccggaagaag tcgccgccat cttcgttgag
     2041 ccgattcagg gcgaaggcgg ctacgtcgtc gcccccaccg tcttcatgca ggagttgcgg
     2101 cgcatctgcg atcgccacgg catcctgctg gtcgccgacg aagtgcagag cggcgcggcc
     2161 cgcaccggca agtggtgggc gatcgagcac tccggcgtcg agcccgatat tgtctgcatg
     2221 gcgaagggca tcgcatccgg catgccgctg ggcatcacga tgtcccgcgc cgaaatcatg
     2281 gactgggtcc ccggttcgca cgcttccacg ttcggcggca acccagtcgc gattgctgct
     2341 gcgctggcca cgcttgacgt gattgagaaa gaacatctgc tcgataacgc agccagcgtg
     2401 ggcggtcaca tcctgaaccg catggcggat tggcctgcga cgctgaagct cgtcggcgat
     2461 gtgcgtggcc gcggtttgat gatcggcgtc gacatcgtaa aggacaaagt cacgagagaa
     2521 tacggtgcag tcgagcgtga ccggatcgtc gaaaaggcgt tcgagcacgg cgtgctgttc
     2581 ctgggctgcg gaccgagttc gatccggctg tgcccgcccc tggtagtcac gaaggaagaa
     2641 gccgatgttg ccgtggacgt tttggaagag tgcatcgcgg cggtgggcaa ggcatagacc
     2701 gttttggatc tgagacacgc gggcaggatc atggatcctg tccgcttttt tcgctgctta
     2761 atcggacgca atcgttcgat tcaggctatt cccactccgg gaattgacga acctcgtccc
     2821 tagccttcct tgtgatgcat atcactttac gcgtgcatcg atgccgctac actggcgcaa
     2881 tgctccccgc cttcttcacg agaaggattg tccggagcct cgattggctg gcagagcgtg
     2941 tacttccacc tgatgaaaag ccggggcacc tgcggaccgg cgcacgaggc gaagaggatg
     3001 cttacttcca cctgcgcaag ctgggttatg tgatcgtggc tcgcaacttt cgctcgccgc
     3061 gctgccctgg agagatcgac ctcatcgggt gggactccga tgttctttgt tttattgagg
     3121 ttaaaacccg cacatcgcgt gacgtaaagc ccgccgaggc ggccgtcgat cgccataagc
     3181 ggcgcgagat cgcggaggta gcacgagagt acctgcggcc tcttccacca tcgtgccagt
//
LOCUS       DASPUL010000685         6743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1913192, whole genome shotgun sequence.
ACCESSION   DASPUL010000685 DASPUL010000000
VERSION     DASPUL010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1913192"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..647
                     /locus_tag="VEJ00_15705"
     CDS             186..647
                     /locus_tag="VEJ00_15705"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXX02662.1"
                     /translation="MRRNSMRLSGLIVTLFLAAVVLGHPTYAQDRKAINLAPAKGLPF
                     SDGVLAGNTLYIAGQEGTDDAGKLASGGIAQETKAALDNVEKVLKAAGFEMKDVVSVT
                     VYLADIHEFGEMNKVYKSAFPDPKPVRATIQAAALVNDARVEISAIAVKQK"
     gene            778..1635
                     /locus_tag="VEJ00_15710"
     CDS             778..1635
                     /locus_tag="VEJ00_15710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXX02663.1"
                     /translation="MRFHSMKVVAALISVAVWAGSFCAARMHAQENAPAKEDLRARSA
                     QGKQIFTANCAGCHGLDGRGGERAPSIVSSAKVRRRSDSQLSNIVSNGIPGTGMPAFR
                     ALPPAQVMALVGYLRTLQGSLGNLANATLPGDPGNGQKVFFGKGECNTCHTISGEGGF
                     LGPDLTAYGATIPAKTIVDAILKPIRVVPPGYRSAVLTTSDGSRLEGIVRNEDNFSVQ
                     LQTSDGSFHFVQKADLQKVEYSSKSLMPVNYGDRLSRSELNDLASYLMTANGSHPAAT
                     ASKQIRDRK"
     gene            complement(1780..3435)
                     /locus_tag="VEJ00_15715"
     CDS             complement(1780..3435)
                     /locus_tag="VEJ00_15715"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HXX02664.1"
                     /translation="MFTHKRYLRIHPDDNAAVALVDLKQGDPVELDGIRADLCSDIPS
                     KHKFALRALSPGDAVILYGTLVGKAVTHISLGELLSTGNTRHDAAPFRSRLHEYRWQA
                     PAINGWESKSFRGFVRSDGQVGTRNYWLVIPLVFCENRNVSVLKQAFEEELGFALPPR
                     YRREVAELVRLFRDGRIDDIRNHAMPEQAALTPRHPVFANIDGIKFLFHNGGCGGTRE
                     DSASLCGLLAGYIHHPNVAGATVLSLGCQNAQVEILRREVEKRNSTFEKPLLVFDQQQ
                     CGTESALLSRTIRETFLGLVEADKSARKPVPLSYLTIGLKCGGSDGFSGISANPAVGH
                     VADLISALHGKALLAEFPELCGVEQSLIDRCHREQDENRFVHLMRAYAERAKAVGSGL
                     EMNPSPGNIRDGLITDAMKSAGAAKKGGTSPITAVLEYPEYATAAGLNLLCTPGSDVE
                     AVTAEVGAGANIVLFTTGLGTPTGNPIAPVVKISSNSQLAARMTDIIDIDAGAVIGGE
                     TTIEAMGEAILDHVIEVASGEIRTKAELLGQDDFIPWKRGISL"
     gene            3664..4440
                     /locus_tag="VEJ00_15720"
     CDS             3664..4440
                     /locus_tag="VEJ00_15720"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXX02665.1"
                     /translation="MGYAGLELDGKVAVVIGGTSGIGQVVALGLAQAGADVVPSSRRS
                     EKVKESAQQIEALGRRSLQITSDVTDRASLQGLLDAVVKNLGKVDILVNAAGRTKRTP
                     TLDVSEQEWNGILDTNLTGALRAGQIFGRHMVERRYGRIIHIASLSSFVALYEVAAYG
                     ASKAALASLTRTMAIEWAAYGVCVNAIMPGVFRTALTSPLLDGTPRGQEFLMRTPMKR
                     FGTLEELVGAAVFLASDAASFVTGTILPVDGGFLASGVNQ"
     gene            4537..5568
                     /locus_tag="VEJ00_15725"
     CDS             4537..5568
                     /locus_tag="VEJ00_15725"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HXX02666.1"
                     /translation="MLRLDPGERRIWTTRTFEVSEGGGEYNVARGLKRCFGLDTAIVT
                     ALADNPVGRLVQDLIYQGGVDQSLVRWVPYDGVGRSVRNGLNFTERGFGVRAALGCSD
                     RGHTAISQVKAGEFDWEHIFGSLGARWFHTGGIFCALSERSPEVAVEAMQAARRHGTI
                     VSYDLNYRESLWKANGGKKRAQEVNRRLAEYVDVMLGNEEDFSAALGFDIPGMDQNLA
                     DFEVESFKKMIQTAVSEFSFKVVATTLRKAKTATRNDWGAICSCQGEFYQATERNDLE
                     ILDRVGGGDSFASGLIYGFLEQKDPQWAVECGAAHGALAMSTPGDTTMVTRDEVLRLM
                     QGASARIAR"
     gene            5591..6259
                     /locus_tag="VEJ00_15730"
     CDS             5591..6259
                     /locus_tag="VEJ00_15730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HXX02667.1"
                     /translation="MKKPHFRKEDVRSRILEVGIVPVIRASSAADGRFAAEAIARGGI
                     PIVEITMTVPGALEVIAQLVSELPELLVGAGTVLDAGMARHCLDAGAQFLVSPGLDLE
                     TVKLAADAKILMMAGALTPTEVMTVWKAGADFVKIFPCAQVGGPAYIKALKGPLPQIP
                     LVPTGGVSLETVAEFISAGAAALGVGGELIKKDAVEARKADTLQNLAQSFVQAVRQAR
                     QRIA"
     gene            6319..>6743
                     /locus_tag="VEJ00_15735"
     CDS             6319..>6743
                     /locus_tag="VEJ00_15735"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HXX02668.1"
                     /translation="MNVKVYETKKLASQAAAAQAAASMAEAIHDRGCARIVAATGTSQ
                     LDFLDALTSTKNVDWQRVELFHLDEYIGLPASHPASFRKYLLERLIRKTGIENYHLLD
                     GDADPREVASRVGQELNSAPVDLALVGIGENGHLAFNDPP"
BASE COUNT         1403 a         1837 c         2116 g         1387 t
ORIGIN      
        1 acggctgcaa agaccgcgcc caaagcggcg atcatcacaa tgcctccgcg gcacctggcc
       61 cagacatgag agttgttcat tgcgtcctcc acgttgtata tatgtcgggg actgtaccat
      121 cgttttctcg tgcttggaag agtgctcgaa gtctgaagca atcggttcgc gaaggaggac
      181 aagaaatgcg acgaaacagc atgcggctgt ctggattgat tgtgaccttg tttcttgccg
      241 cggttgtgtt agggcatccg acttatgcac aggaccgcaa agccatcaac cttgcacccg
      301 caaaaggatt gcctttcagc gacggagttc ttgccggcaa cacgctctac attgcgggcc
      361 aggaaggaac cgatgatgcg ggcaagttgg cttctggagg cattgcgcag gagacgaagg
      421 ctgcgctcga caacgtcgaa aaggtgctga aagcggcagg ctttgaaatg aaggacgttg
      481 tctcggtcac cgtatacttg gctgacattc acgagtttgg cgagatgaac aaggtgtata
      541 agagcgcgtt ccctgatccc aagccagtgc gcgccacaat ccaggcggca gctctggtta
      601 acgatgccag agttgagatt tcggcgatcg cggtgaagca aaagtagtcg atgcacgttg
      661 cttcacgaga atgggtacgg gttccgcgtt tgcagccaga aacgcggcaa gccatagtca
      721 gatcacaaag cggaaagtgt ttagttctag agattgaatg attttcaccg gagagcgatg
      781 cgttttcatt cgatgaaagt agttgctgcc ctgatttccg ttgcggtttg ggcgggatcg
      841 ttctgcgcgg cccgaatgca cgcgcaagag aatgcacctg cgaaagagga cctccgggca
      901 cgctcggccc aggggaaaca gatcttcacg gccaactgcg ccggttgtca cgggctggac
      961 gggcggggag gcgagcgcgc cccgagcatt gtaagcagcg ccaaggtcag gcggcggtcc
     1021 gattcgcaac tctccaacat cgtttccaat ggaataccgg gaaccggcat gccggcgttt
     1081 cgtgccctgc cgcctgcgca ggtgatggct ttagtgggct acctgcgcac cctgcagggg
     1141 agccttggga atcttgcgaa tgcgactctt cccggcgatc cgggaaacgg ccagaaggtg
     1201 ttcttcggca aaggggaatg caacacctgc cacacgattt ccggtgaggg aggatttctg
     1261 gggcctgatc tcaccgccta tggcgcgacc attcccgcaa aaaccatcgt cgatgcgatc
     1321 ctcaagccga tccgagttgt gccgccggga tatcgctcgg ctgtgctaac gacgagcgat
     1381 ggcagccgac tcgaaggcat tgttcgcaac gaagataatt tttccgtgca acttcaaacc
     1441 agcgacggca gttttcattt cgtgcagaag gctgacctgc agaaggtgga gtactccagt
     1501 aagtccctga tgcccgtgaa ttatggcgac cgcctgagtc gctcggaact caacgatctc
     1561 gccagttatc tgatgacggc caatggatct catccagcgg cgacggcctc caaacagatt
     1621 cgagaccgaa aatgagatcg ggctggaaag tggtcttttc gctgacaaca gcggcctgcc
     1681 tggcctcggc tgcgagggct cagcgcaaat tcgaactgct gggactctcg ccctcgttct
     1741 gggatctcgt ggatcacgaa ttctggcggg tgcgccaagt tacagagaga ttcctctctt
     1801 ccacgggatg aaatcatctt gccccagcaa ctcagctttg gtgcggatct ctccgctggc
     1861 cacctcgatg acgtgatcga gaattgcttc gcccatggcc tcaatcgtgg tttcgccgcc
     1921 aatcacagcg cctgcatcga tgtcgatgat gtctgtcatt cgtgccgcca gttggctgtt
     1981 gctcgagatc ttcacgactg gcgcgatcgg attcccggtg ggcgttccca gtccggtcgt
     2041 gaagagaacg atgttagctc ctgcgccgac ttcggcggtg acagcttcga cgtcgctgcc
     2101 cggcgtgcag agcaggttga gtccggcggc agtggcatat tcgggatact cgagtacggc
     2161 agttatcgga gacgttccgc ctttctttgc tgctcctgcc gacttcatag cgtcggttat
     2221 aaggccatca cggatatttc ccggcgaagg attcatttcc aatcctgagc cgaccgcttt
     2281 ggcgcgttcg gcgtatgcgc gcatgaggtg gacgaatcgg ttctcatcct gctcgcggtg
     2341 gcagcggtcg atgagcgatt gctccacgcc gcataactcg ggaaactcgg cgagcagcgc
     2401 tttgccatga agagcggaaa ttaaatccgc cacatgcccg accgccgggt tggctgagat
     2461 gcccgagaag ccgtccgagc cgccacactt cagaccgatg gtcaaatagc ttagcggcac
     2521 tggtttccgc gcgctcttgt cggcctcgac gaggccaagg aaggtctcgc gaatggtgcg
     2581 ggagagcaag gccgattccg ttccacactg ctgctgatca aagacaagca gcggcttctc
     2641 gaaagtggaa ttgcgtttct caacttcgcg ccgaaggatt tccacctgcg cgttctgaca
     2701 accgagactc agaacagttg ccccggcgac gttggggtga tgaatgtacc cggcgagcag
     2761 gccgcacaag gaagcagagt cttcgcgagt gcctccgcag ccgccgttgt gaaacagaaa
     2821 cttgatcccg tcgatgtttg cgaagaccgg gtgccgaggg gtgagtgcag cctgctccgg
     2881 catcgcatgg ttgcgaatgt catctattct gccatcgcgg aacaggcgga cgagttccgc
     2941 gacctcgcgc cggtatcgag gcgggagtgc aaatccgagt tcctcctcaa acgcctgttt
     3001 gagaaccgag acgttgcggt tctcgcagaa gaccagcgga atcacgagcc agtaattccg
     3061 cgtgcccacc tggccgtcag agcgaacaaa tccgcggaag ctcttgcttt cccacccgtt
     3121 tatggcaggg gcttgccatc gatattcgtg cagccgggag cggaatgggg cagcatcatg
     3181 gcgagtgttg cctgttgaca gcagctctcc cagggagatg tgggtgactg ctttgccgac
     3241 caaagttcca tacagaataa ccgcatctcc gggagaaaga gcccgcaggg cgaatttgtg
     3301 cttggaagga atatcagaac agagatccgc gcggatgccg tccagctcaa ccggatcgcc
     3361 ttgcttcaaa tccaccagcg cgaccgcagc gttgtcgtca ggatggattc gaagatatcg
     3421 cttatgcgtg aacactttcc ctcaggctgc gttgttcgtc agtcttattc gtttccacgg
     3481 tgtgaaaatg cttcgtcgaa aagcgcctgt ttgcgtctcg cctcacactg gacgaattct
     3541 acgcagtata tacttgctcg gcgaaagcac tcgaagcagt aagaaagtca agacgaaagc
     3601 agcacgtctt ggtgttcatg aggagagcgg atcttgtcat gaactcaacg ggtggtgaac
     3661 aggatgggtt atgcgggcct ggagttagac ggcaaagttg cggtggtgat cggcgggact
     3721 tcgggcatcg ggcaggttgt ggcactcgga ttggcccagg ccggagccga cgttgtcccc
     3781 agttcgcggc gatctgagaa ggtgaaggag tcggcgcagc agattgaagc gctcggccgc
     3841 cgcagcttgc agatcacttc ggatgtgacg gatcgagcgt cgctgcaggg cctgctggat
     3901 gcggtagtga aaaacttggg caaggtcgac attctggtga acgccgcggg acgcacgaag
     3961 cgcacgccta ccctggacgt cagcgaacag gagtggaacg gaatcctcga cacgaatctt
     4021 accggagcgc tgcgcgccgg ccagattttt ggacgacata tggtggagcg gcggtatgga
     4081 cggatcatcc acatcgcgtc gctgtcgtca tttgtcgcgc tgtatgaggt agcggcttac
     4141 ggagcaagta aagcagccct ggcttcgctg acgcgaacca tggcgatcga gtgggccgct
     4201 tacggcgtgt gtgtgaacgc gatcatgccg ggggtgtttc gaacagcgct cacttccccg
     4261 ctgctggacg ggactccgcg cgggcaggag ttcctgatgc gaacgcccat gaagcgcttc
     4321 gggacgttgg aggagttggt gggtgccgcc gtttttctgg catcggacgc ggcgagtttt
     4381 gtgaccggca cgattttgcc ggtggacggc ggattcctgg ccagtggagt gaaccagtaa
     4441 ggtcacgacg cactttgcga gataggaatg aaagcgcctc ttgcagttaa acccaaagac
     4501 agttgcaagt gggatctcgt cagtcttgga gaggtcatgc tgaggctcga tcccggcgag
     4561 cggcgcatct ggactacacg gactttcgag gtgagcgaag gaggcggcga atacaatgtc
     4621 gcgcgtggac tgaaacgatg cttcggtctg gataccgcaa tcgtcacggc tctcgccgac
     4681 aacccggtcg gccggctggt gcaggatctc atctatcagg gcggcgtcga tcagtcgctg
     4741 gtgcgttggg tgccctatga cggggtcggc cgctcagtcc gcaacggatt gaatttcacc
     4801 gaacgcggct tcggcgtgcg cgccgctcta ggctgctccg atcgcggcca tactgccatt
     4861 tcccaggtca aggccggtga gttcgactgg gagcacatct tcggttccct gggtgcccgc
     4921 tggtttcaca ctggcgggat tttctgcgcg ttatcggagc gatcgccaga ggttgcggtc
     4981 gaggccatgc aggcagcgcg ccggcacggc acgatagtgt cctacgacct gaactacagg
     5041 gagtctctct ggaaggctaa cggaggaaag aagcgcgcgc aggaagtcaa ccggcgcctg
     5101 gcagagtacg tcgatgtcat gctcggcaac gaggaagatt tttcggcggc gctcggcttc
     5161 gacataccgg gcatggatca gaacctcgcc gatttcgagg tagagagttt caagaagatg
     5221 atccaaacgg ccgtgtcaga gttctctttc aaggttgtcg ctacgacgct gcgcaaggcc
     5281 aagacggcga cgcgcaacga ctggggcgcg atctgttctt gccaaggaga gttctatcag
     5341 gcgaccgagc gcaacgatct tgagattctg gaccgcgtcg gcggcggaga ctcctttgcc
     5401 tccggactga tttacggatt cctggagcaa aaagacccgc agtgggcggt ggaatgcggc
     5461 gcagcgcacg gcgcgctggc catgagcacg ccgggcgata cgacgatggt gactcgcgac
     5521 gaagtcctgc ggctcatgca aggagcgagc gcgaggattg cacgctgaga cgggaaagtg
     5581 accgccacgc atgaagaagc cacactttag gaaggaagac gttcgttccc gcattctgga
     5641 agttggcatc gtgccggtca ttcgcgcttc ctcagccgca gatggcaggt tcgcggcaga
     5701 agccatcgcc cgcggcggga tcccgattgt tgaaatcacc atgaccgtgc cgggggcgct
     5761 ggaagtcatc gcccagttgg tcagtgaact gccggaactc ctggtcggtg ccggcacggt
     5821 gctggacgca ggcatggcgc gacactgcct ggatgcgggc gctcaatttc ttgtgagtcc
     5881 cggtctcgat ctggagacgg tgaagttggc ggcagacgcg aagattctga tgatggcagg
     5941 agcattgact ccgaccgagg tcatgacagt gtggaaggca ggcgcggatt ttgtgaagat
     6001 ctttccttgc gctcaagtgg gcggccccgc gtacatcaag gcgctcaaag ggccgcttcc
     6061 ccagataccg ctcgttccca cgggaggagt cagtcttgaa accgtcgccg agtttatttc
     6121 tgccggagca gcggccctcg gtgtaggcgg agaattaata aagaaagacg ctgttgaagc
     6181 acgcaaggca gatacgttgc agaatctcgc gcagagtttc gttcaggcgg tcagacaggc
     6241 tcgccagcgg atcgcctgag gcttgcgcgg cactgtgtgt ggaccgggct caggccatca
     6301 cataccggga agcggaacgt gaatgtaaag gtgtatgaaa ccaagaagct tgcgagccag
     6361 gccgccgcgg cccaggctgc agcttccatg gctgaagcta tccacgaccg cggctgtgct
     6421 cgcatcgtcg cagcgacagg gacctcgcaa ctggattttc tggacgctct gactagcacg
     6481 aagaacgtgg actggcagcg cgtggagctg tttcacctag atgagtacat tggcctgccg
     6541 gcgtcgcatc ctgccagctt tcgcaagtat ttgcttgaaa gattgatccg caagactggg
     6601 atcgagaatt accacctgct agacggagac gccgaccctc gagaagttgc cagccgtgtg
     6661 ggccaagaat tgaattctgc tcccgtcgat ctcgctcttg tcggcatcgg agaaaatggt
     6721 catcttgcct tcaacgatcc ccc
//
LOCUS       DASPUL010000686         2955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1639998, whole genome shotgun sequence.
ACCESSION   DASPUL010000686 DASPUL010000000
VERSION     DASPUL010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639998"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VEJ00_15740"
     CDS             <1..294
                     /locus_tag="VEJ00_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02669.1"
                     /translation="AQEMLDQLARSWPFLMVSMVVGGCFTVLGGYVAGRVARHSFLKH
                     AAIAGALSLVLGILLFRADEGPYSGIVALLGYGLHLPLALLGGWLASRRPVLR"
     gene            1269..2207
                     /locus_tag="VEJ00_15745"
     CDS             1269..2207
                     /locus_tag="VEJ00_15745"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXX02670.1"
                     /translation="MVGFLPATTRAQRSDRIARIGVLVPSISATDSLIEALRTGLHDL
                     GYVEGQNIRIEFRSAQGRFERLPVLAEELARLNVDVIVAGADPAIQAALRATHTTPVV
                     MVGYVYDPVTSGLIDSYGKPGRNLTGVFTRISELVGKRLELLKEMVPNLSRVAVFWDS
                     SGQSELAEVEPAARFLGLHLILIKLHPPYDFASAFRTAKRKKAEAVVLLFSSEFYVRR
                     DQIAASALKFRLPTATGTGEYTEAGGLLSYGPEGVDAYHRAAYFVDRLLKGAKPSELP
                     VEQTAEFKMLVNLKTAEALGLTVPESILLVANKVIR"
     gene            2426..2821
                     /locus_tag="VEJ00_15750"
     CDS             2426..2821
                     /locus_tag="VEJ00_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02671.1"
                     /translation="MNPYTPPDARVADAIQPPDPATRKGGLITQIGTIYLMMGLVLAV
                     LALLRVKIGGYQLAPWNAVTPALLALAGLGAILRQKWGTWSCYFFSVILLLGVPIGTI
                     FGGLMIYHLTIYRAQFDSETGSRARVKVS"
BASE COUNT          567 a          852 c          865 g          671 t
ORIGIN      
        1 gcgcaggaga tgctggatca actcgcccgg tcctggccgt ttctcatggt ttccatggtc
       61 gtcggcggct gcttcaccgt gctcggcggc tacgtcgccg gccgtgtcgc gcgacactct
      121 ttcctcaagc acgcggccat agcgggcgcg ctgtcgctcg tgctcggcat cctgctgttt
      181 cgcgccgacg aggggccata ctcgggcatt gtcgctcttc tcggctatgg cctgcacctg
      241 ccactcgcgt tgctgggcgg ctggctcgcc tcgcgtcgac cagtcctccg ctgaccggtt
      301 gtccgtaagc tgtcggcgct aggctcggcg cctctccgct cgcagcggcg ttacgtcaac
      361 caacgccgtt cgcatcggtc tctcagagga tcggcaatgc agcatcctcg aggtttatca
      421 tgaggaaccc tatgagaaac ccatatgcat aaaccgggaa ggttgagcgg gtttatcatg
      481 gggtctactt gtagttggac cgcttcgcgt tgctgatgcg actacccaca ttatgctggc
      541 tcgcaatcct gctgtggcta agctatggaa ttactctatg gcctcttcct cctcaagaag
      601 ttgctgagac tgtcgccctc tacaagcgat tgatggccga gccctcacct cttgaaaaac
      661 ctgaatggaa gtcccggccg gatctcgaac agaaggaggg gcaacgcaag tacctggagt
      721 tcctggacgt ggcacaagag atcatagacc gcgcacccga actgaaagct cgcctgtggt
      781 gggagtgggc tggtcgcgca gctcttcttg ttgtggccgg attgggctgg ctgtacttag
      841 caaagatccc acgcatatcg agggccgtca ttgcagtcac cattggaatt cttctcatag
      901 ggcagattgt cgtttactgg gtcatttatt ccaaccttgt tcagtcatgg gtcgcgggac
      961 atagcgggat gaagtatcgc cgtgggctaa cgtgctctca atgctttgtc ttctgttcgt
     1021 tctgcccgcc atcctattgc tcgttacagc ccttgcattc cggctggccc cgccccgaga
     1081 ggtctacgca aaatgagcgg tccaacaagc ggctgcagcg gacgcgccga aatgcagcgt
     1141 cgttccttgc tggcgttgtg cgggcgcgcc actgagccgc ggcgtttggc catcaagtgg
     1201 taaatcacgg agcgcgtggg taaggtaatc agatgatacg aaggctcgtc ctagtcggca
     1261 tcgcgctgat ggttggtttc ttgcccgcta cgactcgtgc tcagcgttca gatcgtatcg
     1321 cgcgcatcgg agtactagtg ccttccatta gcgcgactga ttcgttaatc gaagcgttgc
     1381 gcacagggct gcacgacctc gggtatgtcg aagggcagaa tatccgaatt gagtttcgaa
     1441 gtgcacaagg tcggtttgaa cgattgcctg tcctggctga agaattggcg cgtctgaacg
     1501 tcgacgtgat tgttgctgga gcggacccag caattcaggc cgcgctacgg gctacgcata
     1561 cgactccggt cgtcatggtc ggctacgtct acgatcccgt cacctccggc ctcatcgaca
     1621 gctacggaaa gcccgggaga aacctcacag gcgtgttcac ccgcatatcc gagctggtcg
     1681 gtaaacggtt ggaactgctg aaggaaatgg ttccgaacct gtctcgcgtg gccgttttct
     1741 gggactcctc tggtcagagc gaactcgccg aggtcgagcc tgcggcacgg ttcttaggtc
     1801 tgcacctcat cttaatcaag ctgcaccccc cgtacgattt tgcgagtgca tttaggacag
     1861 cgaagaggaa aaaggcggaa gcagtcgtac tgctgttttc atccgaattt tacgtgcggc
     1921 gggaccagat tgccgcttcg gcactgaagt ttaggctgcc tacggctacc ggaaccgggg
     1981 agtacaccga ggctggaggc ttgttgtcct atgggccaga gggagtggac gcttatcata
     2041 gagctgcata ttttgtggac cggctgctca aaggcgcgaa gccgagcgaa cttcctgtcg
     2101 agcagacggc cgagttcaag atgcttgtca acctcaagac agcggaagcc cttggcctaa
     2161 cggttccgga gtcgattctg ctggtcgcga acaaggtaat tcgataggcc atccatttga
     2221 tgtattcgcc aacgcgaaaa tgacgcccaa cgagcgcgcc aacacggact cacaaacagc
     2281 cggctttggc ccgctgctca acaagacctt gcaccctact cctaagcggc agctccgctt
     2341 ggtccccgtc gcgcatcgcg caccgacggc gggtcaagac cgtcctcgcg cgtcatgaat
     2401 ggtgctcgcg gttggaagga tctggatgaa tccctacacc ccgcctgatg ctcgggtcgc
     2461 ggacgcgata cagccgcctg acccagcaac gaggaagggt gggcttatca cccagatcgg
     2521 caccatctat ctgatgatgg gactggtgtt ggctgtcctt gcgcttctcc gcgtcaagat
     2581 cggaggttat caactagctc cttggaatgc ggttaccccc gctctcctgg ccctcgctgg
     2641 tttgggcgca attcttcgcc agaagtgggg tacatggtcc tgctatttct tctctgtgat
     2701 attgcttctt ggcgtcccga tcggcacgat ttttggcggt ttgatgatct atcacttgac
     2761 gatctaccga gcgcaatttg actccgagac tgggagccgt gcacgagtaa aggtgtcgtg
     2821 aagtccagcg aatggcaaga ctctatctga tccgacacgc acgcccgaca gcaagctggg
     2881 ctgaagcgac cgatccgggt ctcgacgtca ccggccacca gcaagctgag gcggccgctc
     2941 agatgcttgc aaaga
//
LOCUS       DASPUL010000687         3529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1366926, whole genome shotgun sequence.
ACCESSION   DASPUL010000687 DASPUL010000000
VERSION     DASPUL010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366926"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(546..2096)
                     /locus_tag="VEJ00_15755"
     CDS             complement(546..2096)
                     /locus_tag="VEJ00_15755"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HXX02672.1"
                     /translation="MDLEQKVAELKRRNQLAEDGGGAKRRERQHKEGKMSARERIEFL
                     LDEGSFEETDKLVTHRCNDFGMAEQKIYGDGFITGYGRIEGRLVYVFAQDFTVFGGSL
                     SEANAGKIVKIMDLAAKMGAPVIGLNDSGGARIQEGVMSLAGYADIFLRNTIYSGVVP
                     QISAIMGPCAGGAVYSPAITDFVLMVDKTSYMFITGPDVIKTVTLEEVTKDQLGGAMT
                     HNETSGVAHFLAHDDAECLSMVRELLSFVPSNNLDDPPRKACTDPADRADAALDTIVP
                     AQSNLPYDIKNVIHAVVDDGYFFEVQEHYAKNIVVGFARLDGRSVGIVANQPAVLAGT
                     LDINASVKGARFVRFCDCFNIPLVTFEDVPGFLPGTAQEYGGIIKHGAKLLYAFAEAT
                     VPKLTVITRKAYGGAYCVMASKHIRTDVNYAWPTAEIAVMGPEGAVDIVYKRELDAAA
                     NREQVRKEKIEEFRDRFANPYVVADRGFVDAVIQPRETRKKLIQALAMLETKRDKNPP
                     KKHGNIPL"
     gene            2325..2816
                     /locus_tag="VEJ00_15760"
     CDS             2325..2816
                     /locus_tag="VEJ00_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02673.1"
                     /translation="MSSKFWLRLGVSTVLSLALSGAIAVAQDHDRDDDRWHDHGRDHE
                     RDHDRDRDHDRNFDHDRGHHEGYVDHDRDIRGWYRAHYDHLPPGLAKRDRLPPGLERR
                     LVVNGFLTDDLRRRMQPCPHALEVMLPPPAPGFAHVFIGGNLVLVNRANFQVVDVFHF
                     NLN"
     gene            2956..>3529
                     /gene="xseA"
                     /locus_tag="VEJ00_15765"
     CDS             2956..>3529
                     /gene="xseA"
                     /locus_tag="VEJ00_15765"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXX02674.1"
                     /translation="MSSVADQMGFTFRAPERRVWKVRDLVAAVRTHIEREYSDAWVEG
                     EVSNFRAPESGHLYFTLKDGNAQIRVVMFRTSARLLRFRPVDGLQVIVRGRVTVYEER
                     GELQISAEYMEPRGAGSLQLAFEQLKAKLEAEGLFAAERKKPIPSLPSQIGIVTSVQA
                     AALRDILNVLARRHHSVNVLIYPAQVQGDAA"
BASE COUNT          682 a         1113 c          983 g          751 t
ORIGIN      
        1 acagctaact ttgcatgatc taagaaattc tctctgcacc atttccggtc acagtcctgt
       61 gaacggcctg cactcgattg aaaatccatg tacttacacg ctacgatttt tcgttgactc
      121 cgaaactgca acccggtaaa aacacaatcg ttctttgaca ccttcttaag tttttccggt
      181 tggtgcggcg tccggggctc gagcgaagtc accttcatga ccgtgaggtt gaggaggagg
      241 gtgagcaagg gtctatagca aagcgctggt cgaggagaac gagaagacca agtcagaggc
      301 aggatagagt tccgaggcgt tgggttggtt tttccggttg gcggtgagcc cggagcccga
      361 gccaagatcg cgcagagcag ccccgcaagg gaagctttga gagcaggtcg agcgagggtc
      421 agaagcgagc tactggccga gaggaatgga tcccacacca cctcggaact cttattttct
      481 tggtctgtag ggcgacccac cctctgctgt ctagcccctc gaagcctgcg atccgcagtg
      541 cccacctata gcggtatatt cccgtgcttc ttcggcggat tcttgtctcg cttggtttcg
      601 agcatggcca gcgcctggat cagcttcttg cgcgtctccc gcggctggat gacagcgtct
      661 acgaatccgc ggtcagccac gacgtagggg ttggcgaagc gatcgcggaa ttcctcgatc
      721 ttctccttcc gcacctgctc gcgattggcc gcagcgtcga gttcgcgctt gtacacgatg
      781 tcgaccgcac cctctggtcc cataacggca atctccgccg tcggccaggc atagttcaca
      841 tcggtacgaa tatgcttcga cgccatgacg caatatgccc cgccgtaggc cttgcgcgtg
      901 atcaccgtga gttttggcac ggtcgcctcg gcaaaggcgt aaagcagctt tgccccgtgc
      961 ttgatgatgc cgccatattc ctgcgccgtt cccggcagga atcccggcac atcttcaaac
     1021 gtcaccagcg gaatattgaa gcagtcgcaa aaacgtacga accgcgcgcc cttcactgac
     1081 gcgttgatat cgagcgtccc cgccagcacc gccggctggt tggccacgat ccccacgctg
     1141 cgcccgtcga gccgtgcaaa tcccactacg atgttcttcg cgtaatgctc ctgcacctcg
     1201 aagaaatatc cgtcgtcaac tacggcatgg atcacgttct ttatgtcgta cggcaaattc
     1261 gactgtgcag gcacgatcgt gtcgagcgct gcatcggccc gatccgccgg atcggtgcag
     1321 gccttccggg gcggatcgtc gagattgttc gaaggcacaa aactgagcag ctcgcgcacc
     1381 atcgacagac attcagcgtc gtcatgcgcc agaaagtgcg ccacgcccga ggtttcgttg
     1441 tgcgtcatcg cgccgccgag ctggtccttg gtgacttcct caagcgtcac cgtcttgatc
     1501 acgtccggcc cggtaatgaa catgtaggaa gtcttgtcga ccatgagtac aaagtcggtg
     1561 atcgcgggcg agtaaacggc acctcctgcg cacggcccca tgatcgccga gatttgcggc
     1621 accacgccgc tgtagatcgt gttgcgcaga aagatgtcgg cgtagcccgc gagtgacatc
     1681 acgccttcct gaatgcgggc acctccggaa tcgttcagcc cgatcaccgg tgcacccatc
     1741 ttcgccgcca gatccatgat cttgacgatc ttccccgcat tcgcttccga gagcgatccg
     1801 ccgaacacgg tgaaatcctg cgcgaacaca taaacgagcc ggccttcgat gcgaccgtat
     1861 cccgtgataa acccgtcgcc gtaaatcttc tgctcggcca tgccgaaatc gttgcagcgg
     1921 tgcgtaacca gcttatcggt ctcctcgaac gagccttcgt cgagcaggaa ttcgatgcgc
     1981 tcccgcgccg acatcttgcc ctctttatgc tgtcgctcgc gccgcttggc gcctccgccg
     2041 tcttcggcca gctggttgcg tcgttttagt tccgcgactt tctgttccag gtccatagtt
     2101 ttgaagtctc tgggcggaaa ttatagcttc tgaacccgtt tgaagccgcc ggcagcgccg
     2161 cctcacagcg tccgaatgcc acaaacgccg cccgattgcg tggaggacac aacggcaaga
     2221 tccatcgaat gacgaacatc ccgcgacagt gaccggtcgt aatctgtcgc tgcggcttgc
     2281 tcgccggtac gcttgagttc ttccaaagaa tttaggagga atctatgtct tcaaagttct
     2341 ggctgcgcct tggcgtcagc acagttctgt cgttggcgct ctcaggcgct atcgcagtgg
     2401 cgcaagacca cgatcgcgat gacgaccgct ggcacgatca cggccgcgac catgagcggg
     2461 atcatgatcg cgacagggac cacgaccgca acttcgacca cgaccgcgga caccatgaag
     2521 gctacgttga tcacgaccgc gacatccgtg gctggtaccg tgcccactat gaccatctgc
     2581 cgcccggcct agccaaacgc gatcgtttgc ctccgggcct ggagcgaaga ctggttgtca
     2641 atggcttcct gaccgacgat ctgcgccgca gaatgcaacc ttgcccgcat gcattggaag
     2701 tgatgcttcc cccgcctgcc ccggggtttg ctcatgtctt catcggcggc aatctggtgc
     2761 tcgtcaatcg tgcgaatttc caggtcgtcg acgtcttcca cttcaatctg aactaagcca
     2821 caaacctggg ttgcccactt ctcgcgcgtt ctgtgcgcga gaggtggggg ttccccccgg
     2881 gaaccaagcc tcccgccatg cgctgtcgca ccaatggaag agacttgccc ctccgcctgc
     2941 ggtacactgc ccccaatgtc ctccgtcgcc gaccagatgg gattcacctt ccgcgcgccc
     3001 gagcgccgcg tgtggaaagt tcgtgacctg gttgccgccg tccgcaccca catcgagcgc
     3061 gagtattccg acgcctgggt cgaaggcgaa gtctcaaatt tccgcgcgcc cgaatccggc
     3121 catctctatt tcacgctgaa agacggcaat gcgcagattc gcgtcgtgat gttccgcacc
     3181 tcggcgcgcc tgttacgctt tcgcccggtc gacggcttgc aggtcatagt gcgcggccgt
     3241 gtcaccgttt acgaagaacg cggcgaactg cagatttcgg ccgagtacat ggagcccagg
     3301 ggtgccggat cacttcagct tgccttcgag cagcttaagg ccaagctaga agccgaggga
     3361 ctgtttgccg cagaacggaa gaagccgatc ccctcgttgc cctcgcagat tggcattgtg
     3421 acgtccgtgc aagccgcagc gctgcgcgac attctgaatg tgctcgcacg ccgtcatcat
     3481 tcggttaacg tgctcatcta tcccgcgcag gtgcaaggcg acgcggcgg
//
LOCUS       DASPUL010000688         2917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1644489, whole genome shotgun sequence.
ACCESSION   DASPUL010000688 DASPUL010000000
VERSION     DASPUL010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1644489"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1290)
                     /locus_tag="VEJ00_15770"
     CDS             complement(145..1290)
                     /locus_tag="VEJ00_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXX02675.1"
                     /translation="MAENELQQIVERAVAKVLDQHLPKLQAELVKNVLAELPASVSAP
                     ATPSSSGSTASAGGLVQAVAGIHAGTTQKEILKALLDAGSAYAARIALFVVKAGAANG
                     WQCRGFEGDETIKDFSLDLSAGPAAHAYQNRVVTPGNIAEMDSRFVERFGAPENEQVL
                     VLPLVLKDKVAALVFADGGAGGVLDSASLELLVLATSAWLEVASLRKQAQREADSAFE
                     RGAERAMERPAAAPVQTVSSFPDPFAAHAPKHTAPPVPEPEPAAEVVEVSAHAAAAGA
                     ASAPAAAADPFAGMSAEDADTHRKAQRFARLLVDEIKLYNQAKVAEGRRHKDLYDRLK
                     DDIEKSRATFQKRYGATAAGSGDYFQKEVVRSLAEDDLSVMGASFRK"
     gene            1568..2197
                     /locus_tag="VEJ00_15775"
     CDS             1568..2197
                     /locus_tag="VEJ00_15775"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXX02676.1"
                     /translation="MGAIQNQLSPPVTDELSLVQAAKRGDDSAFEELVRRYDRNVFRI
                     AQHITQNREDAEDVVQEAFLKAYSNLAQFQEQSKFYTWLVRIAVNEALMKLRRRKPER
                     TVSLDEEIKTEDDSLPREIADWSPNPEQQYTQAELREILSKTIQGLPPGFRTVFVLRD
                     VEGLSTEETASALELSVPAVKSRLLRARLQLRDRLGRYFQKRESGDGNR"
     gene            2194..2454
                     /locus_tag="VEJ00_15780"
     CDS             2194..2454
                     /locus_tag="VEJ00_15780"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HXX02677.1"
                     /translation="MKCSEFLQELTNYLDGVLDEKTKVELENHLSWCHNCYVVCDTTK
                     KTIEIYRDSQLYELPEDLRSRLRSAIMTKCQQQKKRGPQSVG"
     gene            2717..>2917
                     /locus_tag="VEJ00_15785"
     CDS             2717..>2917
                     /locus_tag="VEJ00_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXX02678.1"
                     /translation="MFVELYESIFGCRHSHYSFPVTIRRAARRPQAAALTGTYVVCLD
                     CGKEFPYDWQEMKVITSPEQHRA"
BASE COUNT          589 a          885 c          870 g          573 t
ORIGIN      
        1 ccagggcgat ggcccaagac agcttgcggg gtgctcgcgg caacggccgt ttttgcggag
       61 atgcgagagc cagaaccttt ttgagagacg ccagcactgc tttcagacta ccccagggag
      121 caaactgtgt ctcgactcag acgcctactt gcggaagctc gcgcccatga ccgagagatc
      181 gtcttcggcg aggctgcgta cgacttcctt ctggaaataa tcgccgctgc cagctgcggt
      241 cgcaccatag cgcttctgaa acgtggcgcg gctcttctcg atgtcgtcct tgaggcgatc
      301 gtagagatct ttgtgacgtc ggccctcggc gactttcgcc tggttgtaga gcttgatctc
      361 atcgactagc aagcgcgcga agcgctgcgc cttgcggtgc gtgtcggcgt cctccgcaga
      421 cattccggcg aagggatccg cggccgctgc cggcgcgctc gccgcgcctg ccgctgccgc
      481 gtgcgcggaa acttcgacca cttccgccgc cggctcgggc tcgggcaccg gcggggccgt
      541 gtgcttggga gcgtgggctg cgaaaggatc gggaaaagaa cttaccgtct gcaccggagc
      601 ggccgcaggc cgttccatgg ctcgttccgc accacgctcg aaggcggagt cggcttccct
      661 ctgcgcctgc ttgcgcaggg aggccacttc cagccacgcg ctggtcgcca gaaccaggag
      721 ttcaagcgag gccgagtcca gaacaccgcc cgctcccccg tcggcaaaaa ccagagccgc
      781 cactttgtct ttcagcacca gcggcagcac cagaacctgc tcgttctcgg gtgctccgaa
      841 gcgctcgacg aaacggctgt ccatctcggc gatgttgccc ggagtgacca cgcgattctg
      901 gtaggcgtgg gctgcaggcc cggcgctcag gtcgagggaa aagtctttga tggtctcgtc
      961 accttcaaaa ccgcggcact gccagccgtt ggccgctcct gcttttacca cgaacagggc
     1021 gatgcgcgct gcgtacgcgc ttccggcatc gagcagagcc ttgaggattt ccttttgggt
     1081 ggttcccgcg tgaatgccag cgacggcctg aaccaggcct cccgcgctgg ctgtcgatcc
     1141 tgaactggag ggcgtggcag gagcagagac cgacgctggc agttccgcca gcacgttctt
     1201 aacgagctca gcttgtaatt tcggcagatg ctggtcgagg accttggcca ccgcacgttc
     1261 tacgatttgc tggagttcgt tttccgccat gctcagtgga ccgcccctac tcccggttgc
     1321 cccgcctctg ttgaacgaca atagtagcgc cgtaactgct gattatacgg aacttgtctg
     1381 gactcggcag ggtactatag tacctgctcg cccggggcgg agcgggttgc atccgacttt
     1441 ctgcactcta tcgcaaactt tccggcttga atacgtctaa ggaattgggc aaagcgccgg
     1501 gttaccaagt agaataacag ttccgactgg ggcggccgct tctcgaaaat actgcatcgc
     1561 ctgaactatg ggagccatcc aaaatcaact gagtccacct gttaccgacg aactgtcgct
     1621 ggtacaggcg gccaagcggg gcgacgattc cgccttcgaa gaactggttc gccgctacga
     1681 ccgcaatgtg tttcggatcg cccagcacat cacgcagaac cgcgaagacg cggaggacgt
     1741 ggtgcaggaa gcgttcctga aggcatacag taacctggcg caatttcagg agcagtcaaa
     1801 gttctacacc tggctggtac ggatcgccgt caacgaagcg ctcatgaagt tgcgccggcg
     1861 caagccggag cgcaccgtat cgctggacga agaaatcaaa acggaagatg actccctccc
     1921 ccgcgaaatc gccgattgga gccccaatcc ggagcagcag tacacgcagg cggagttgcg
     1981 cgagattttg agcaagacca tccagggcct ccccccgggc tttcggacgg tgttcgtact
     2041 gagggacgtg gaaggacttt ccaccgaaga aactgcgagc gcgctggaac tgagcgttcc
     2101 cgcggtcaag tcgcggctgc tgcgggctcg cctgcagctg cgcgaccggc tgggacgcta
     2161 cttccagaaa cgtgagagtg gagatggcaa ccggtgaaat gctctgaatt tttgcaggag
     2221 ctgaccaatt acctggacgg cgtcctcgac gaaaagacta aggtggagtt ggagaatcat
     2281 ctctcctggt gccacaactg ctatgtagtc tgcgacacaa ccaagaagac catcgagatc
     2341 taccgcgatt cccagctcta cgaacttcct gaagacctcc gcagccgcct ccgctccgcc
     2401 atcatgacca agtgccagca gcagaaaaag cgcggcccgc agagcgtcgg atgagtgaaa
     2461 aggttttccc cgcggctcta actatttcca tcgtaacgat ttcgcttgtt ccctttcctg
     2521 cactctcctg ccactagaaa gccgaaaccc ttcgcttcat ctctgaatct aagtaataga
     2581 gtgaaagcgt gcgcggtctg aggcgcagag ggggcgcttt ctaatacctg gccaccatgc
     2641 aacgaagtgg aggcagttgg gtcggtataa ccagggaccc gggcacacca gcccggcagg
     2701 ggaaagggta ggtaccatgt ttgtagaatt gtacgagtcg atttttggct gcagacactc
     2761 gcactacagc tttcctgtca cgatccgtcg ggcagcgcgc cgcccccagg ccgccgcttt
     2821 gaccggaacc tatgtggttt gtctcgattg cggcaaagag tttccctacg actggcagga
     2881 aatgaaggtg attacgtcgc cggagcagca tcgcgca
//
LOCUS       DASPUL010000689         3216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U12302
            k141_1918731, whole genome shotgun sequence.
ACCESSION   DASPUL010000689 DASPUL010000000
VERSION     DASPUL010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945218
            Sequence Read Archive: SRR7004340
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Ma,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 04:27:01
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,161
            CDSs (total)                      :: 3,128
            Genes (coding)                    :: 3,118
            CDSs (with protein)               :: 3,118
            Genes (RNA)                       :: 33
            tRNAs                             :: 31
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 0 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 1 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918731"
                     /isolate="SMAG_U12302"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="Colombia"
                     /lat_lon="3.8381 N 73.3194 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..1452)
                     /locus_tag="VEJ00_15790"
     CDS             complement(325..1452)
                     /locus_tag="VEJ00_15790"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXX02679.1"
                     /translation="MSRFGRSVNAPAITDALSCVSVYNFSPATGQRESPLPSSDPISV
                     AVVGVGVFGRNHARVYKELELQGKGVRLLGVVDPDVNRADTVAREFSCKAFGSVKQML
                     TTHSELQAASVAAPTVHHLPVARDLMKAGVDVLIEKPLAASVAEAEQLAEIADKHKRI
                     AQVGHLERFNPAVRATLPLLTQPMFFEVHRLSVFTPRSLDVDVVLDLMIHDLDIVLAF
                     ANSPVKEVRAVGLPILSGKVDIANVRLELESGCVANFTASRVSTERVRKLRFFQPRQY
                     VSLDYGRQEVLVFTVGQDGKPSGLPSPNPQIGVTKPPVASEEPLHAELASFLQAVRER
                     SMPVVPLADGLRALAVALQIVSAIRDHGKKIDLAGIAQGKS"
     gene            1489..1815
                     /locus_tag="VEJ00_15795"
     CDS             1489..1815
                     /locus_tag="VEJ00_15795"
                     /inference="COORDINATES: protein motif:HMM:NF016245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HXX02680.1"
                     /translation="MKTIVLLTISNIFMTFAWYGHLKYREVPLYKVIAVSWLIALFEY
                     CFQVPANRIGSYEFSAAQLKTIQEVITLTVFAVFSVLYLRQPVRWNHVAGFGLIVAAV
                     AVIFKQ"
     gene            complement(1805..2356)
                     /locus_tag="VEJ00_15800"
     CDS             complement(1805..2356)
                     /locus_tag="VEJ00_15800"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXX02681.1"
                     /translation="MKTKRAGLKSESALIADQLHRAFYGDAWHGPALLELLEDVDAAT
                     ASVKPLGDVHSIWELVLHIAAWDGAGIRRLAGKKTQLKGKQNFPPVPEPATGAAWREA
                     VERAQSTHDALVKTVAGLSASRLCDIVPGKRYNFCHMLHGIAQHELYHAGQIAILKKV
                     QPKQDMPRPEARPESPDHRSVTA"
     gene            complement(2376..>3216)
                     /gene="lpxI"
                     /locus_tag="VEJ00_15805"
     CDS             complement(2376..>3216)
                     /gene="lpxI"
                     /locus_tag="VEJ00_15805"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HXX02682.1"
                     /translation="TDVQRIGLIAGNGKFPFLVLDAARAQGYEVIVAAIKEEAFPEIE
                     SHGPAAVYWLSLGELSKLIEAFQREGVRRAVMAGQVRHKQIFSSIRPDWRLAKLLLSL
                     TTRNTDSLLGAVAQVLAEEGITLEKSTWLLEPLLVKPGVLTKRSPSEPERKNIDYGRA
                     VARQLAQHDIGQTVVIAESACVAVEAMEGTDATIERAGQIMRSLQGEASTLERALTVV
                     KVAKPNQDMRFDVPVIGVKTIEVMRAAGATCLALDAGRSLFLDGDKVAEAASAAGIAI
                     VAD"
BASE COUNT          618 a          974 c          962 g          662 t
ORIGIN      
        1 caggtcccga taggcggctt cccagtcatt tgtgtcgtaa agtccgagct gctcgggttg
       61 cgaggggtcg gccggactta ccgggtcctg gaatggaatc tgcgtaatcg gcatgcgctg
      121 gcttcggcta cggacacccg cttgaaacgc tggtcgttag acacgaaatc ccctaacggc
      181 gttgcatccg gccttctagc aaatcgatgc tgggcgcgcg gtgcgtttac aaccgggttg
      241 gctctatttt cgcatctttc gtgccattct gctcagcgca aatcaggtgc gacctcggtg
      301 tgtaaacata tgtaaaacgg gatcttagct tttaccctgg gcgatgccag ccagatcaat
      361 tttctttccg tgatcccgga tggccgagac gatctgcagc gctacggcaa gagcgcgcag
      421 tccgtcggcc agcggaacca ccggcattga ccgctctctg acggcctgca ggaaggaggc
      481 gagttcggcg tgcagtggtt cctccgaggc taccggaggc ttggtgacgc cgatctgcgg
      541 attcggggaa ggcaagccgg agggcttgcc gtcctgcccg acggtaaaga ccagaacttc
      601 ctgccgcccg tagtcgaggg agacgtattg ccgcggctgg aagaaacgca gcttccgcac
      661 gcgctcggtc gacacgcggc tggccgtgaa gttggcaacg caccccgact ccagctccag
      721 gcgcacgttc gcaatgtcca ccttgccaga aaggatgggc aagccgacgg cgcgaacttc
      781 cttcacgggt gaattggcaa atgccagcac gatgtcgagg tcgtggatca tgagatcgag
      841 aaccacgtcg acatcgagcg atcggggcgt gaatacgctc aggcggtgca cttcgaaaaa
      901 catcggctgg gtcagcaacg gaagcgtggc ccgcaccgct gggttgaaac gttcgaggtg
      961 gccaacctgg gcaatgcgtt tgtgcttgtc ggcgatctcc gccaactgct ccgcttcggc
     1021 aacggatgcc gccagcggct tctcaatcag cacgtcgacg ccagccttca tcagatcacg
     1081 cgctacaggc aggtgatgca ccgtgggagc tgcaaccgat gcggcctgca gttcgctgtg
     1141 cgtggtgagc atttgcttga ccgagccgaa cgccttgcag ctgaattcac gggcgacggt
     1201 gtcggcacga ttcacgtcgg ggtccactac gcccagcagg cgaacacctt tcccttgcag
     1261 ttcgagttcc ttgtagactc gcgcgtgatt gcgcccgaac acgcccacgc ccacgacagc
     1321 gacggaaatc ggatcggaag atggcagcgg agattctctt tggccggttg ccggagagaa
     1381 attgtaaacg gaaacgcagg aaagggcatc tgtaatagca ggcgcgttga ccgagcgccc
     1441 gaatctactc acgcgagcgc gcaaattcag aaaggacaag gccgaccaat gaaaacgatc
     1501 gtgctgctca ccatttcaaa tatcttcatg accttcgcct ggtacggcca cctgaaatac
     1561 cgcgaggtgc cgctctacaa ggtgatcgcg gtgagctggc tgattgcgtt atttgaatac
     1621 tgttttcagg tgccggcgaa tcgcattggg tcgtacgagt tcagcgccgc ccagctcaaa
     1681 accattcagg aagtaatcac gctcacggtg ttcgctgtgt tctcggtctt gtatctgagg
     1741 caaccggtgc gctggaacca cgtcgccgga ttcgggctga tcgtcgccgc tgtagcggtg
     1801 attttcaagc agtgacagag cggtgatcag ggctctcagg ccgggcttca ggccggggca
     1861 tgtcttgttt tggctgcacc ttcttcagta tggcgatctg cccggcgtgg taaagctcat
     1921 gctgggcgat cccatgcagc atgtggcaga aattgtagcg ctttccggga acgatatcgc
     1981 acaagcgcga cgccgagagg ccggcgacag tcttcaccaa ggcgtcgtga gtgctctgcg
     2041 cccgttcgac cgcctcgcgc catgccgcac cggttgctgg ttccgggacc ggtggaaagt
     2101 tctgtttccc tttgagctgg gttttcttgc ctgccagacg ccgtatgccg gcgccgtccc
     2161 acgccgcaat gtgcaacaca agttcccaga tgctgtgcac gtcaccgagc ggcttaaccg
     2221 aggccgttgc cgcgtcgaca tcctccaaga gttcgagcaa ggccggccca tgccaggcgt
     2281 cgccgtagaa ggcgcgatgc aattggtcgg caatcagggc ggactcagat ttcagtcctg
     2341 cgcgcttcgt tttcattcgg caccattctt taccttcagt ctgcgacgat tgcgattccg
     2401 gctgcgctcg ccgcctcggc aaccttgtct ccgtccagga aaagagatct gcccgcatcc
     2461 aacgccaggc aagtagcgcc ggcagctctc atcacctcga tcgtcttcac cccgataacc
     2521 ggcacatcga accgcatgtc ctgattcggc ttggcaacct tcaccacagt cagagcgcgc
     2581 tccagtgtgg aagcttcccc ctgcagggac cgcataatct gcccggcacg ttcgatcgtg
     2641 gcatcggtgc cttccatcgc ttcgacggcg acgcatgcag attcggcaat gaccaccgtc
     2701 tgcccaatat catgctgggc cagttggcgg gcgacggctc gtccgtagtc gatgttcttg
     2761 cgctccggct cgcttgggct ccgttttgtc agtacgccag gtttgaccag caacggctcg
     2821 agcagccacg tcgacttctc gagcgtgatc ccctcttcag ccaggacctg cgccaccgcc
     2881 cccagcagtg agtcggtatt gcgggtggtc agcgaaagca agagcttcgc caggcgccag
     2941 tctggtcgaa tgctcgagaa aatctgcttg tgcctgactt gcccggccat cacggcgcgc
     3001 cgtacgcctt ctcgctggaa tgcttcgatc agcttggaca attcacccaa agagagccag
     3061 taaaccgcgg cgggcccgtg ggactcgatc tccgggaatg cttcttcctt gatggccgcg
     3121 acaataacct cgtacccctg cgcgcgcgcc gcgtcgagca cgaggaaggg aaatttgccg
     3181 ttgccggcga tgaggccaat gcgttgcacg tcagtg
//
